LOCUS       DASTMT010000001         2019 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_1641440, whole genome shotgun sequence.
ACCESSION   DASTMT010000001 DASTMT010000000
VERSION     DASTMT010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2019
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1641440"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            106..1071
                     /locus_tag="VFR00_00005"
     CDS             106..1071
                     /locus_tag="VFR00_00005"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEU0157656.1"
                     /translation="MTNGFAAFVDWRRLAGVLALTLPPLLVLPLLVGEFSAHVLAISA
                     YYVILAASWNMLAGFTGQFSLAQHAFAAIGAYGSGLLVYHLKAPIWLSIPLGVCAAGA
                     GGFLLGILVLHMRAIYLSIATWAFAETLRIFLAAAYEFTRGDLGLSVPSLYGHVRPVI
                     YYYTFLGTAVLCLIGVYLILKSPIGYFMRAVRDDPLRARSLGVDTTRVKLFAFVASSA
                     MAGLAGALYAHYILTLTPSIVDFSEMARIVVMVVVGGLGSFWGPIIAAPPIYALNIWL
                     AAWGEWSTVFFAAIVIALMRAYPAGLAGLIELGAHRWRRRQAPPL"
     gene            complement(1068..>2019)
                     /locus_tag="VFR00_00010"
     CDS             complement(1068..>2019)
                     /locus_tag="VFR00_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018455927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HEU0157657.1"
                     /translation="QITAQPRLTFDRLELLDGVGLVPIVMGLFGVAEILSNLEQELKR
                     EVIKARIGGLWPSMQDWAQSKWAIVRGTLLGFFLGILPGGGAVIASFASYAIEKKVSK
                     TPERFGHGAIEGVAAPEAANNAAAGGAFIPLMTLGIPPNVVMALLLGAFIIHGLQPGP
                     LLMTQRPDVFWGIVASMYIGNAMLLVLNMPLIGMWVQVLKLPYRILFPLILMFCIVGV
                     FASGNASFDVLLMVIFGVLGYLMRKFGYEPAPMVLAFVLGPMLENNLRKSLILSHGDF
                     QIFIARPISAACLLLAVAALAAPLLPALARRRATIAASET"
BASE COUNT          340 a          657 c          643 g          379 t
ORIGIN      
        1 cgaccatctc tatctcttga acctcgggcg cgtgaaggcc gagggcccgg gacgcgagtt
       61 cgacaatcaa cgcgtgagaa ggctggttca ggaatgcctc ctcggttgac aaacggcttt
      121 gccgccttcg tcgactggcg ccgccttgcc ggtgtcctgg ccctgacgct gccgccgctg
      181 ctggtcctgc cgctgctcgt cggcgagttc agcgcccacg tgctcgccat cagcgcctat
      241 tacgtcattc tggccgccag ctggaacatg ctggcgggct tcacggggca gttctcgctg
      301 gcccagcacg cctttgccgc catcggcgcc tacggctcgg gtctcctggt ctatcacctc
      361 aaggcgccga tttggctcag cattccgctt ggcgtttgcg cggccggcgc gggcggcttt
      421 ctgctcggca tactggtttt gcacatgcgc gccatctacc tgtcgatcgc cacctgggcg
      481 tttgccgaaa cgttgcgcat ctttctagcc gcggcctacg agttcacgcg cggcgatttg
      541 ggattgagcg tgccgtcgct ctatggccac gtgcggccgg tcatctatta ctacaccttc
      601 ctgggcacgg ccgtgctgtg cctgatcggg gtctatctga tcttgaagtc gccgatcggc
      661 tatttcatgc gcgcggtgcg cgacgatccc ctgcgcgcgc gctcgctcgg ggtcgatacc
      721 acgcgggtga agctgttcgc cttcgtcgcc tcgagcgcca tggccgggct cgccggcgcg
      781 ctttatgccc actacatact gacgctgacg ccctcgatcg tcgacttcag cgaaatggca
      841 cgcatcgtcg tgatggtcgt ggtcggcggg cttggctcgt tttgggggcc gatcatcgcc
      901 gccccgccga tctatgccct caacatctgg cttgccgcct ggggcgaatg gagtacggtc
      961 ttttttgccg ccatcgtcat cgccttgatg cgtgcctacc cggcgggact ggccggtctc
     1021 atcgagctcg gcgcgcatcg ctggcggcgg cggcaagccc ctccccttta agtctccgac
     1081 gcggcgatcg tggcgcgccg gcgcgccaac gccggcagca agggagcggc gagagcggcc
     1141 accgccagca gcagacaggc ggcggagatc ggtcgcgcga taaagatctg aaagtcgccg
     1201 tgcgacagga tcaaggattt tcgcaagttg ttctcgagca tcggtcccag cacgaaggcc
     1261 agcaccatgg gggccggctc atagccgaac ttgcgcatca ggtagcccag cacgccgaat
     1321 atcaccatca acaacacgtc gaaactggcg ttgccggagg cgaacacccc gacgatgcag
     1381 aacatcaaga tcagcgggaa taggatgcgg tagggcaatt tcaggacctg cacccacatg
     1441 ccgatcagcg gcatgttgag cacgagcagc atggcgttgc cgatgtacat gctggcaacg
     1501 atgccccaga agacatccgg gcgctgggtc atcagcagcg ggccgggctg caggccatgg
     1561 atgataaagg cgccgagcag cagggccatg accacgttgg ggggaatgcc gagcgtcatc
     1621 agcgggataa aggcgccacc ggcggctgca ttgttggcgg cctcgggggc cgctacaccc
     1681 tcgatcgcgc cgtgaccgaa acgctcgggc gttttcgaca cctttttctc gatcgcatag
     1741 ctggcgaagg aggcgatcac cgcgccgccg ccgggtaaaa tgccgaggaa aaagccgagc
     1801 agcgtgccgc gcacgatcgc ccatttcgat tgcgcccagt cctgcatgga aggccacagc
     1861 ccgccgatgc gggccttgat cacctcgcgc ttgagttcct gctcgaggtt ggacaggatc
     1921 tcggccacgc caaacagccc catcacgatc ggcaccaggc caacgccgtc gaggagttcg
     1981 agccgatcga aggtgaggcg cggctgcgcc gtgatctga
//
LOCUS       DASTMT010000002         2619 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_5906718, whole genome shotgun sequence.
ACCESSION   DASTMT010000002 DASTMT010000000
VERSION     DASTMT010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2619
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5906718"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1921
                     /locus_tag="VFR00_00015"
     CDS             <1..1921
                     /locus_tag="VFR00_00015"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEU0157658.1"
                     /translation="NDKYSDAKPHAILAFSADSLDAAVSYRKLLGSKTPIVFCVLPPA
                     GTTVNLEPDMAGIISEHDVSKTVALARRLQPKARDLVVVAGAAAFDRKGAMIAKQQLA
                     NVEKQLSTRYLVGLPDETVQRELANVSRNTIIVLLSKLTEAPGRGAAAGDAASIIGAA
                     NAPIYAPYSTYFGRGIVGGYMDTFEGMGTQTADLVLNILEGRETVASGPRKSTSQAFR
                     VDARQLERWGLSSQDLPSGAVVAHKSPTLWESHPRIVTATIAMFTAMASVLMALLVQI
                     GRRKLAEANLKDSEKRMRFAAAAADIGLWQYDIQARRLWSSKHCRAMLGLPVREPLTT
                     AALISRVHPDDRHLARASIRAATYGTLNDGESEFRVVWPNGDTRWLQARGRTDLDENG
                     EPLRASGIFRDITNYKIAQLEAKHLSQRVLSIQDDERQRIAQDLHDSTSQHLAAIGLN
                     LIALKGSKGSCSVLEDIENSLEEASKELRTFTYLLHPPALADDGLIGTLARYVEGFRR
                     RTNLAVSLRSAGCIEELSRLAQESILRVVQEALANVHRHAKASCATVHLRRVGGHLHV
                     VVSDDGIGLKASGTLAQGERLPTGVGIAGMTARMRQLGGSLQIRCKPMGTVVHAVVPI
                     GAAQGAVPEHQIAAE"
     gene            2338..>2619
                     /locus_tag="VFR00_00020"
     CDS             2338..>2619
                     /locus_tag="VFR00_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007800049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper oxidase"
                     /protein_id="HEU0157659.1"
                     /translation="MYLPKDASRSRIREAQKARENRAEIVRALSHGQISRRELFKWGL
                     FTSSGLLLWKNGLNVYAPSAYGQVPTGTPRSPLFGARKFTQPFRRLSLQT"
BASE COUNT          498 a          870 c          825 g          426 t
ORIGIN      
        1 caacgacaaa tatagcgacg ccaaaccgca tgccattttg gccttcagcg cggattcgct
       61 cgacgccgct gtgagctacc gcaagctctt gggcagcaag accccgattg tcttttgcgt
      121 gctccccccg gcggggacca ccgtcaatct cgagcccgat atggccggca ttatcagcga
      181 gcacgatgtc agcaaaaccg ttgcgctggc ccggcgcttg cagcccaagg cacgcgacct
      241 ggtcgtggtg gccggcgcgg ccgccttcga ccgcaaaggg gccatgatcg cgaaacagca
      301 gctggccaac gtcgaaaagc agctgagcac gcgctatctg gtcggcctgc ccgacgaaac
      361 cgtgcaacgg gaactggcga acgtctcgcg caacaccatc atcgtgctgt tgtccaaact
      421 gaccgaggca cccggccgcg gcgccgccgc cggcgatgcg gcctccatca tcggcgcggc
      481 caacgccccg atctacgcgc cctatagcac ctacttcggt cgcggcatcg tcggcggcta
      541 catggacacc ttcgaaggca tgggcacgca aacggccgac ctggtcctca acattctgga
      601 aggccgtgaa accgtcgcca gcgggccgcg caagagcaca tcgcaggcct ttcgcgtcga
      661 tgcccggcaa ctcgagcgct ggggtctgtc gagccaagat cttcccagcg gcgccgtcgt
      721 tgcgcacaag tcgccaaccc tgtgggagag ccatccgagg atcgtgacgg caaccattgc
      781 catgtttacg gccatggcct cggtgctgat ggcgctgctg gtgcaaatcg gcaggcgcaa
      841 actcgccgaa gccaatctta aggacagcga gaagcgcatg cggtttgcgg ctgccgccgc
      901 cgatatcggc ctatggcagt acgatatcca agcgcggcgc ctgtggtcgt cgaagcactg
      961 ccgcgccatg ctggggctgc ccgtgcgcga gccgctgacg accgcggcct tgatctcgcg
     1021 ggtgcacccc gacgaccgcc atctcgccag ggcttcgatc cgcgcggcaa cctacggtac
     1081 cttgaacgat ggcgagagcg agttccgcgt cgtttggccc aatggtgaca cgcgctggct
     1141 gcaggcgagg gggcgcaccg atttggacga aaacggcgag ccgctgcggg ccagcggcat
     1201 tttccgcgac attaccaact acaaaatcgc ccagctggaa gccaagcact tgtcgcagcg
     1261 ggtcttgagt attcaagacg acgagcgcca acgcatcgcc caggacctgc atgattcaac
     1321 ctcccagcat ctggcggcga tcggcctcaa tctgatcgcg ctcaaaggat cgaagggctc
     1381 ctgcagcgtg cttgaggaca tcgagaactc gctcgaggag gcctcgaagg aattgcgcac
     1441 cttcacctat ctgctgcatc cgccggcgct cgccgacgat ggcctgattg gcaccctcgc
     1501 gcgctacgtc gagggcttca ggcggcgcac caacctggcg gtcagcttgc gatcggccgg
     1561 ctgcatcgag gagctgtcgc ggctcgcaca ggaatcgatc ctgcgtgtcg tgcaggaggc
     1621 gctcgccaac gtgcatcgcc acgccaaggc ctcgtgcgct acggtgcatt tgcggcgcgt
     1681 cggcggtcat ctgcacgtcg tcgtcagcga cgatggcatc ggcttgaagg catcgggcac
     1741 gctggcgcaa ggcgaacgcc tgccgaccgg cgtgggaatt gccggcatga ccgcacgcat
     1801 gcggcagctg ggtggcagcc tgcagatccg ctgcaagcca atgggaaccg ttgtgcacgc
     1861 cgtcgtgcca atcggcgccg cgcaaggcgc cgtgcccgag catcagatcg ccgccgaata
     1921 aacacctcgc cctccgcgcg ccgcccaaca tccgcgacaa gcgaaggccc ggcgcgcgcc
     1981 acgtggttgc acgcgatccg aacgccgcga tggcgacgcg agtcccaagg gaaagttgag
     2041 cattatcaac attcagcccc ttggtcccat agattttaga caaaggtccg cagcgccctc
     2101 gaccaagcgg cacctatagc tgggttggga gcctgaaagg tctcccatga ggtagctgtg
     2161 catgaacgcg gaaggcggcg gtgctgtcgc cgacgcgtgg cgactgatct cagggccggg
     2221 ccaatccgtt gccagcggct tgaggcgtgt ggggcacccg ccggcagctc cctcccgcgc
     2281 taatggcacg ccttgagatc gaggttagga cgcgcattgg gctcgaggaa taaacccatg
     2341 tatttgccga aggacgcatc gcgcagtcgc atcagggaag cgcaaaaggc gcgcgagaac
     2401 cgcgccgaaa tcgtgcgcgc gctttcgcac ggacagatca gccgacgcga gctgttcaaa
     2461 tgggggctgt tcaccagcag tggtctcttg ctgtggaaaa acggcctcaa cgtctatgcg
     2521 cccagcgcct atggccaggt gccgaccggt accccgcgca gtccgctgtt tggcgcccgc
     2581 aagttcaccc agccgtttcg ccggctcagc ctgcagacg
//
LOCUS       DASTMT010000003         8249 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_1313498, whole genome shotgun sequence.
ACCESSION   DASTMT010000003 DASTMT010000000
VERSION     DASTMT010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 8249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8249)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8249
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1313498"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..485
                     /locus_tag="VFR00_00025"
     CDS             <1..485
                     /locus_tag="VFR00_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009796841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU0157660.1"
                     /translation="GWSREPVVELLIPSVLDDTLAPPGAHVASLFVQHVAPELPAARR
                     WESDKEAFADLVIETITAHAPNFKASVLGRQSLSPLDLERRFGLVDGDIFHGQLTLDQ
                     LFSARPVLGHADYRMPVAQLYLCGSGAHPGGGVTGLPGRNAAREIARDFKAVGRRWRG
                     "
     gene            585..989
                     /locus_tag="VFR00_00030"
     CDS             585..989
                     /locus_tag="VFR00_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009799534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HEU0157661.1"
                     /translation="MSPDRPRPSARPRPQIMSLTDRAAERITAILAGKGTGAAVRIGV
                     KKGGCAGMEYTMEWAGEPQPHDEVVEHKGARVLIDPKAVMYLLGAEMDYRVDKLAAQF
                     VFSNPNQSSACGCGESVNLTPASTERLAALRG"
     gene            complement(1083..1778)
                     /locus_tag="VFR00_00035"
     CDS             complement(1083..1778)
                     /locus_tag="VFR00_00035"
                     /inference="COORDINATES: protein motif:HMM:NF014813.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009404 - toxin metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-activating lysine-acyltransferase"
                     /protein_id="HEU0157662.1"
                     /translation="MIFGSRKSKDTPAAPVAPPIANGKAQDAGAAERPIAPPRPAVIP
                     SASAKPAAAPASDAAGTPTSDEARRRAAAAVRHSLAFAQIVSILMHSPRYRHYTLGDL
                     EWLVLPPLLTGQCSVAEAKSKDNGASVPVAVALWASVSPEVDQRLTENLNTPIRLRPD
                     EWRSGEILWLIDAVGDRRVLPGLLKQLADNTLKGREIKVRGRGEGGKVEVKNLQATLQ
                     AAAAAGPPAGATS"
     gene            complement(1824..3125)
                     /locus_tag="VFR00_00040"
     CDS             complement(1824..3125)
                     /locus_tag="VFR00_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019197107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HEU0157663.1"
                     /translation="MPISGTKAAIQRHLWAGLTLLLVLCGGVGGWAATTEISGAVIAP
                     GVLVVDTNVKKVQHPTGGVVGEIRARDGDRVTIGDIVVRLDETVTRANLGIVLRGLDE
                     LVARKARLEAERDGLEGIAFPSEFIERLGELQVATIVAGERKLFDLRQSARIGQKSQL
                     RERIAQLREEIGGIAAQIKAKAQEIVLIQRELTGARDLWEKNLMPITKLTQLEREATR
                     LEGERAQLTATSAQSKGKISELELQIIQVDRDLASEVGKELREVDAKIGEFVERKVAA
                     EDQLKRIDIRAPQDGVVHQSIVHTIGGVINAGEQLMLIVPSADNLIVEAKFAPQDIDQ
                     VKVGQRAVLRFTSFNQRTTPELNGVVTRVSADTTVDQRTSAPYYTLRISLSREEIARL
                     GEVTLVPGMPVESFIQTGERKVISYLMKPLSDQIMRAFRER"
     gene            complement(3125..5209)
                     /locus_tag="VFR00_00045"
     CDS             complement(3125..5209)
                     /locus_tag="VFR00_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018643374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion system permease/ATPase"
                     /protein_id="HEU0157664.1"
                     /translation="MLAVRLLGGGLFAAALISLVLDVRHCLRAGTGFALTSLENLWFA
                     AHPDSLRTAQTLLLEAAPPWIWAYLVRPLLLIPIALLGGLFGYLLVTIGRRQRRRRAS
                     AEDRGLPIALRDLGQQPGSAGSALASALASCRGAFVGIALFSGMSNILLLTGSMFMLE
                     VYDRVLPSRSIPTLIGLAILTAVLFMAQGGFDLIRSRLLSRVGATLDETLSPRVYDTM
                     VHLPVRMGGRADSLQPLRDLDSLRSFLSSLGPTALFDLPWMPLYLTIIFAFHWILGVT
                     ALVGAILLVCLTVLTEIMTRKPTKAAIEFAVSRQRLADSSRRNAEVMVAMGLTQRVGA
                     HWRDANRNYIAAHQAASDVGGGIGAVSKVMRLMLQSAMLGIGAYLVIMQEATGGIIIA
                     GSILSARALAPVDLAIAHWKGFASARQSWQRLSKLLAMIPSAGEAMALPVPTNTFAVE
                     NISIVPPGEQKFVVTDITFALNRGQGLGIIGPSASGKSCLARALVGAWLPARGKIRLD
                     GAALDQWDPIALGRHVGYLPQDVELFAGTVAQNISRFDDHADPELIVRAARAADVHEL
                     IVRLPNGYNTQIGEQGATLSAGQRQRIGLARALYGDPFLVVLDEPDANLDSEGMLALT
                     QAILSVRTRGGIVIVIAHRHNVLTNVDLLLAMNLGRALAAGPKDAVMKKLRLAAPGME
                     PLKVVAEAGRSG"
     gene            5515..6081
                     /locus_tag="VFR00_00050"
                     /pseudo
     CDS             5515..6081
                     /locus_tag="VFR00_00050"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
     gene            6243..6614
                     /locus_tag="VFR00_00055"
     CDS             6243..6614
                     /locus_tag="VFR00_00055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0157665.1"
                     /translation="MVLNAGFAAGPVSMDAVEAGAADSFAAAGADAGAIVAYVRSIGL
                     KAADVQRLLVHAPDGSILVDHRADALSRNEAQSLLFAGKQRPPGGWPAGVYRASYRVL
                     AHGVPVLERSFSLTLPAARAP"
     gene            6695..7810
                     /locus_tag="VFR00_00060"
     CDS             6695..7810
                     /locus_tag="VFR00_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009493425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HEU0157666.1"
                     /translation="MPTYRNLQPGDPAPWFHQRSSVNANYAFDSAAGRYLVLCFFLSA
                     ANERAQAALQAASARRALFDDDKAAFFGVSIDPADEAQRRVATRLPGYRYFWDSDGKV
                     AKLYGALPVEAQAPGAGARQMWVVLDPTLRILKVTPFSEDGAANAEIFAFLDALPPPA
                     RFAGIALQAPVLVLPNVFEAEFCERLITLYNSSGGQESGFMREKDGKTVIAHDYGHKR
                     RKDHEIVDLEVIKHAQNRVIRRVAPEILKVHQFQVTRMERYIVSCYAVEDGGHFAPHR
                     DNTTKGTQHRRFAVSVNLNADFDGGEVSFPEYSKQGLKPPPGGAVVFSCSLLHTVSKV
                     TRGRRYAFLPFLYDEAAAKIREANNAFLGEGVGAYQS"
     gene            7856..>8249
                     /locus_tag="VFR00_00065"
     CDS             7856..>8249
                     /locus_tag="VFR00_00065"
                     /inference="COORDINATES: protein motif:HMM:NF023426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 1"
                     /protein_id="HEU0157667.1"
                     /translation="MRILFVHQNFPGQFPHLAPALSARGHDVSALTVAGNKRPSPVPV
                     FHYTYQKPNFERAISRHVGNFADRAERGAAVALAAEKLRANGYVPDLVFGHFGWGETL
                     YLKNVWPSSKLALYAEFFYHPRGLDADFD"
BASE COUNT         1374 a         2807 c         2655 g         1413 t
ORIGIN      
        1 aagggtggtc gcgcgagccc gtggtcgagc tgctgatccc ctccgtgctc gacgatacgc
       61 tggcgccgcc gggagcccat gtcgccagcc tgttcgtgca gcacgtggcc cccgagctgc
      121 ccgcagcgcg gcgttgggag agcgacaagg aggccttcgc cgacctggtc attgagacca
      181 tcaccgcaca tgcccccaac ttcaaggcca gcgtgcttgg ccgccaaagc ctctcgcccc
      241 tcgacctgga acgccgcttc ggccttgtcg atggcgacat cttccacggc cagctgacgc
      301 tcgaccagct cttcagtgcc cggcccgtgc ttgggcacgc cgattaccgc atgcccgtgg
      361 ctcagcttta tctgtgcggc tccggggccc atcccggcgg cggcgtcacg gggctgccgg
      421 ggcgcaacgc ggcccgggag attgcacgcg atttcaaggc agttgggcgg cggtggcggg
      481 gctgagccct gcgcctgccg cggggccagg ccctcgattt tttacgcaca tacgctatat
      541 ttgtggctgt tggcccgaac cgccatcgcc gccttgagat ccgcatgagc cccgatcgtc
      601 cgagaccctc cgcccgcccg cgaccgcaaa tcatgagctt gaccgaccgg gccgccgaac
      661 gcatcacggc aatcctggcc ggcaagggca cgggggcggc cgtgcgcatc ggcgtcaaaa
      721 aaggcggctg cgccggcatg gaatacacca tggagtgggc cggggagccg cagccgcacg
      781 atgaggtcgt cgagcacaag ggcgcccggg tgctgatcga cccgaaggcg gtgatgtacc
      841 tgctcggggc ggaaatggac taccgcgtcg acaagctcgc cgcccagttc gtgttctcca
      901 acccgaacca gtcaagcgcc tgcggctgcg gcgaatcggt gaatttgacc cccgcgagca
      961 cggaacggct ggccgcgctg cgcggctgag ccgcgctcga agacgccgtt cgtgacgcgg
     1021 gcggcgtgcg cgcgccccga gtgcgcgccg ccatgccggc caagcgcgtt cctgttttcc
     1081 gcctaggagg tcgcccctgc cggtggtccg gccgcggcgg cggcctgcaa cgtggcctgc
     1141 aggttcttga cctcgacctt gccgccctcg ccgcgcccgc gcaccttgat ttcgcgtcct
     1201 ttcaaggtgt tgtcggccag ttgtttgagc agccccggca acacgcggcg gtcgccgacc
     1261 gcatcgatca gccagagaat ttcccccgag cgccactcgt ccgggcgcag gcggatgggg
     1321 gtgttaaggt tttccgtcaa gcgctgatca acctcgggcg atacgctggc ccacagcgcg
     1381 accgccaccg ggacggaggc gccgttgtct ttggacttgg cctccgcgac gctgcactgc
     1441 cccgtcagca gcggcggcag caccagccac tcgaggtcgc ccagcgtgta gtggcgatag
     1501 cgcggcgagt gcatcaggat gctgacgatc tgcgcaaagg ccagcgagtg gcgcaccgcg
     1561 gcggcggcgc ggcggcgcgc ctcatccgag gttggggtac ctgcggcgtc gctggccggt
     1621 gcggccgccg gcttggcgga tgcggacgga atgaccgcgg gccgcggcgg cgcaatcggc
     1681 cgctcggcag caccggcatc ctgcgccttg ccattggcga tgggcggcgc cacgggtgcg
     1741 gccggggtat ccttggattt cctggatccg aatatcatct cgcaactccg ttactcacac
     1801 ctacgttcaa ggcgcccgtg attttatctc tcgcggaaag ctcgcattat ttggtcggag
     1861 aggggcttca tcaagtacga tatcactttt cgttcgccgg tttgaataaa cgattcgacc
     1921 ggcatgccag gcaccagcgt gacctcgcca agccgggcga tttcttcgcg gctcagggaa
     1981 atcctgagcg tgtaataggg cgcagaggtg cgctggtcca cggtggtatc ggccgatacg
     2041 cgggtgacca caccgttcaa ctcaggggtt gtccgctggt tgaagctcgt gaatctcagc
     2101 accgcccgct gcccgacctt gacttggtcg atgtcctgcg gtgcgaattt cgcctccacg
     2161 ataagattgt ccgctgaagg aacgatcaac atcagctgct cgccggcatt gatgacgccg
     2221 ccgatggtat gcacaatcga ttgatgtacg acgccgtcct gcggggcacg aatgtcaatg
     2281 cgctttaatt gatcctcggc ggcgaccttg cgctccacga actcgccgat tttggcatcg
     2341 acctcccgca actccttgcc gacctcgctg gcaaggtcac ggtcgacctg aataatctgc
     2401 aactcgagct cggaaatctt gcccttactc tgggcgctgg tggccgtcag ctgagcgcgc
     2461 tcgccctcga ggcgggtcgc ctcgcgttcg agctgcgtca gcttggtgat tggcatcagg
     2521 ttcttttccc agaggtcgcg ggcgccggtg agctcgcgct gaatcagcac gatctcctgg
     2581 gccttggcct tgatctgcgc ggcgatgccg ccgatctcct cgcgcaactg ggcaatgcgt
     2641 tcgcgcagct gcgatttttg cccaatgcgc gcggattgcc gcaggtcgaa cagcttgcgc
     2701 tcgccggcga cgatggtggc cacctgcaat tcgcccagcc gctcgatgaa ctcgctggga
     2761 aaggcaatgc cctcaagccc gtcccgttcc gcctccaggc gcgccttgcg cgccacgagc
     2821 tcgtcgaggc ccctcagcac aatgccgagg ttggcccgcg tcacggtttc gtcgagacgc
     2881 accacgatgt cgccgattgt gacgcgatcg ccgtcgcggg cccttatttc gccgaccacg
     2941 ccgcccgtcg ggtgctgcac cttcttgacg ttggtgtcca ccaccagcac ccctggggca
     3001 atgaccgcgc cggagatttc cgtggttgcc gcccagccgc cgacgccgcc gcacagcacg
     3061 aggagcaggg ttagtcccgc ccagagatga cgctggatcg ccgccttggt tccgctgatg
     3121 ggcatcagcc gctcctcccc gcctcggcca cgaccttgag cggctccatg ccgggcgcgg
     3181 ccagcctcag cttcttcatc acggcatctt ttggtcccgc cgccagcgcg cgcccgagat
     3241 tcatggccaa cagcaggtcg acgttggtca gcacgttgtg ccggtgggca atgacgatga
     3301 cgatgccgcc gcgtgtgcgc acgctgagaa tggcctgggt cagcgccagc atgccctccg
     3361 agtcgaggtt ggcgtcgggc tcgtccaaca ccaccaggaa cgggtccccg tagagcgcgc
     3421 gcgccaagcc gatgcgctgg cgctgcccgg ccgacaaggt tgcgccctgc tcgccgatct
     3481 gagtgttata gccgttcggc aaacgcacga tcagttcgtg cacgtcggcg gcgcgcgccg
     3541 cgcgcacgat cagctccggg tcggcatgat cgtcgaaacg agaaatgttc tgtgccacgg
     3601 tgccggcaaa cagttcgacg tcttgcggca gatagccgac atggcggccg agcgcgatgg
     3661 gatcccactg gtcgagggcg gcgccgtcga ggcggatctt gccccgtgcc ggcagccagg
     3721 cgcccaccag cgcgcgtgcc agacaggatt tgcccgaggc gctgggaccg atgatgccga
     3781 ggccctgacc gcggttgaga gcaaacgtga tatcggtgac gacgaacttc tgctcgcccg
     3841 gcggcacgat ggaaatattc tccaccgcga aagtgttggt gggcaccggc agcgccatcg
     3901 cctcgcccgc agacgggatc atggccaaca gcttgctcag ccgctgccag ctctggcgcg
     3961 ccgaagcaaa gcccttccag tgcgcgatcg ccaaatccac cggcgccagg gcccgcgccg
     4021 agaggatcga gccggcgatg atgatgccac ccgtcgcctc ctgcataatc accaggtagg
     4081 ccccgatccc cagcatggcc gactgcaaca tcaggcgcat gaccttggag accgcgccga
     4141 tgccgccacc cacgtcgctg gccgcctggt gcgcggcgat atagttgcgg ttggcatcgc
     4201 gccagtgcgc accgacccgt tgggtcagcc ccatggcgac catcacttcg gcattgcgtc
     4261 ggctcgagtc cgccaatcgc tggcgcgaca cggcaaattc gatggcggcc ttggtcggct
     4321 tgcgcgtcat gatttccgtg agcacggtga ggcaaacgag cagaatggca ccgaccagcg
     4381 ccgtgacccc caaaatccaa tgaaaggcaa agatgatcgt gaggtagagc ggcatccagg
     4441 gcaggtcgaa cagcgccgtg ggaccgaggc tggagaggaa agagcgcaga ctgtcgaggt
     4501 cgcgcagggg ctgcaggctg tcggcgcgcc cgcccatgcg caccggcaga tgcaccatgg
     4561 tgtcatagac gcgaggactg agcgtctcat cgagggtcgc cccgacccgg ctcagaaggc
     4621 ggctgcggat caggtcgaag ccgccctggg ccatgaacag cacggccgtc aaaatcgcca
     4681 agccgatcag ggtgggaatg ctgcggctcg gcagcacgcg atcgtacacc tccagcatga
     4741 acatggagcc ggtcagcaac aggatgttgc tcatgccact gaagagggcg atgccgacaa
     4801 aggcgccgcg gcaggacgcc agcgcgctcg ccagcgccga gccggccgag cccggctgct
     4861 gcccgagatc ccgcagcgcg atcggcagac ccctgtcctc ggcactggcg cggcggcggc
     4921 gctgccggcg cccgatggtg accaagaggt agccgaaaag gccgcccagc aaggcgatcg
     4981 ggatcagcaa caggggccga acgaggtaag cccaaatcca gggtggagcg gcctcaagca
     5041 gcagcgtctg cgccgttcgc agactgtctg ggtgcgccgc aaaccaaagg ttctccaggc
     5101 ttgtcagcgc aaacccggtc cctgcccgca ggcagtgcct cacatcgagg accaatgaga
     5161 tcaacgcggc ggcgaagagt ccgccaccca acaaccgcac cgccagcata ccccaacaca
     5221 ccctacgctt gatcgtacac ccctagccga ggctgcgcac cacagccgcg acctatcaat
     5281 ggatgtctat ccgggttttt ttcttaatcc acgaattccc ctccagctaa tctaccacct
     5341 gtgctcggga agtggcaatg cgagccacac ggttcgcctc gcccgctgcg gatttttggc
     5401 atcataccga ggcgcaaccc gatgatggcc caggatgaac gcagctcgtt gtgcattttc
     5461 gaggccaacg ccctgctcca tagtgggaca gcgcgcgtgg gcgcacctga ccgcatgcgc
     5521 tgggtcgacg acgtgacccg atggcgccgc tcgcaacgtt gcccagcggc gcgcgagacg
     5581 cagaaaagtg ttgttgtgca ccgccttaag ctcgccattg cgctcctgct gagcctcgcg
     5641 ctcgacgggc tcggcgcgct tcgggcggcc gacgggctcg tcctcgacct gccgctcgcc
     5701 tgtacgctgg gcgagacttg tgcgatccag cagtacgtgg accacgatcc ctcgccggcg
     5761 gcgcgggatt atcaatgtgg aacgctgtcc tatgatggtc ataatggaac ggatttccgc
     5821 cttcccagcc tggcggcaca acgggccggc gtcgacgtgt tggcggctgc ccccggcgtg
     5881 gtcttgcgcg tgcgcaacga catgaacgac ctcgccatgg ccgaaggcgg tcgcgtcgcg
     5941 ctcggcgccc ggctgtgcgg caacggcgtc gtcatcgccc accgcgaggg ttgggagacc
     6001 cagtactgtc acctcgccaa ggccagcgtg cgcgttgctc ccggccaaag cgtgggcgcc
     6061 ggaaccgcgc tcggccgagt cggtctttcc ggcgcgacgg tcttcccgca cctgcacttc
     6121 accgtgcgtc accacgggct atcgttgacc cgttcgcttt cgcggcgccg gcggagagct
     6181 gcgggggcgg tacatccctg tggcgaccga ccctggcgac cggcctcgcc tatcgggccc
     6241 gcgtggtgct caatgcgggc ttcgcagcgg gccccgtcag catggacgcg gtggaggcgg
     6301 gcgcggccga cagctttgca gcggcgggcg ccgatgccgg tgccattgtc gcctatgtgc
     6361 gcagcatcgg cctcaaggcg gccgacgtgc aacggttgct cgtgcacgcc cccgacggca
     6421 gcattctcgt cgaccaccgg gccgacgcgc tcagccgcaa cgaggcgcag tcgctcctgt
     6481 ttgccggcaa acagcgcccg ccaggcggct ggccggccgg ggtttatcgg gccagctacc
     6541 gcgtgctggc gcacggagtg cccgtgcttg agcggagctt ttcgctgacg ctgcccgcgg
     6601 cacgggcacc gtgaaccatg tttgcgggcg gcttaggcgc ccagcttcaa atagccaacg
     6661 gcctgggtta ggttgccgcg tttgggagtt cttcatgccg acctaccgaa atcttcagcc
     6721 aggcgacccg gcgccttggt tccaccagcg ctcgtccgtg aatgcgaact acgctttcga
     6781 ttcggcggcc gggcgttacc tcgtgctgtg ctttttcttg agcgctgcca atgagcgcgc
     6841 ccaggcggcg ctccaggccg cctctgcccg tcgcgcgctg ttcgacgacg acaaggcggc
     6901 ctttttcggg gtcagcatcg atcccgccga tgaggcgcaa cggcgggtgg cgacccggct
     6961 gcccggctac cgttattttt gggacagcga cggcaaggtc gccaagcttt acggcgcgct
     7021 gcccgtcgag gcacaagccc cgggcgccgg tgcgcggcaa atgtgggtcg tactcgaccc
     7081 gacgctgcgc atcctcaagg tcaccccctt cagcgaggac ggcgccgcta acgcagagat
     7141 tttcgccttt ctcgacgcgc tgccaccgcc cgcccgcttt gcgggcattg cgctgcaggc
     7201 cccggtgctc gtgcttccta acgtgttcga agcggagttt tgcgagcgcc tgatcacgct
     7261 ctacaacagc agtggcgggc aggagtcggg cttcatgcgc gaaaaggacg gcaagaccgt
     7321 gatcgcgcat gactacggac acaaacgccg caaagaccac gagatcgttg acctcgaggt
     7381 gatcaagcac gcgcaaaatc gcgtcattcg gcgcgttgcc ccggagatcc tgaaggtgca
     7441 ccagttccag gtcacccgca tggaacgcta catcgtcagc tgctatgcag tggaggacgg
     7501 tggccacttt gcgccgcacc gcgacaacac caccaaggga acgcaacacc gccgctttgc
     7561 cgtatcggtc aatctcaacg ccgacttcga tggcggcgag gtgagctttc cggagtattc
     7621 caagcagggt ctcaaaccgc cgccgggcgg ggcggtcgtg ttctcctgct cgctgctgca
     7681 taccgtgtcc aaagtcacgc gcggccgacg ctacgccttc ctaccattcc tttatgatga
     7741 ggcagcggcc aagattcgcg aggccaacaa cgccttcctc ggcgaaggcg tgggcgccta
     7801 tcagagctga tcagcgccgg cgcgccccgg caacctcaag aggttcggcc cgcgcatgcg
     7861 catcctgttc gtgcaccaaa acttccccgg ccagtttccg cacctggcgc cggcgctcag
     7921 cgcacgcggg cacgatgtca gcgccttgac ggtcgccggc aacaagcggc cctccccggt
     7981 tcccgtcttc cactacacct accaaaagcc caatttcgag cgcgcgatct cgcgccacgt
     8041 cggcaacttc gccgaccgtg ccgagcgcgg ggccgcggtc gcccttgccg cggaaaaatt
     8101 gcgcgccaac gggtacgtcc ccgacctggt attcgggcat ttcggctggg gtgaaacgct
     8161 ttacttgaag aacgtctggc cgagcagcaa gctcgcgctt tatgcggaat tcttctacca
     8221 tccgcgtggt ctggatgccg atttcgacc
//
LOCUS       DASTMT010000004         4504 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_3939187, whole genome shotgun sequence.
ACCESSION   DASTMT010000004 DASTMT010000000
VERSION     DASTMT010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4504
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3939187"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..772
                     /locus_tag="VFR00_00070"
     CDS             <1..772
                     /locus_tag="VFR00_00070"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HEU0157668.1"
                     /translation="HQRPAGASNLMLGDLWGSDVLATTSRGAGNTLAMAEYALAGILH
                     FAKGLQRAAVDREAGAFQHRAYRPLLLEGKTALIVGAGGIGLEVGRLCAALGMRVVGT
                     RRTPAAGAALPTGFRRLGTPAELDGFLRESDVVAICCQWTPETDRLFNAQRFALMKKG
                     SILVNVARGEIIDEAALLEALQRDQLRGVVLDVYTGEFEHPPPQGLWRDPRVLVTPHI
                     SGASDQDRHGAIAIFCENLRAYLDGQPLRNLIDWTRGY"
     gene            complement(787..1539)
                     /locus_tag="VFR00_00075"
     CDS             complement(787..1539)
                     /locus_tag="VFR00_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015933944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU0157669.1"
                     /translation="MQAFDSDGVSIRFIDEGSGDPVLLVHGFASSVKYNWIDPGWVSL
                     LRRNAYRVIAYDNRGHGESAKLYDPQAYQPASMMEDARRLLDHLGIARADVMGYSMGA
                     RLTAFFALAHPERLRSAIFGGLGGNMIRPMAGTGPIAHALEAKSIDEVVNPTARTFRA
                     FAEKTGSDLAALAACIRGSREPISREMVAKISCPVLVATGTEDVIGGSAAELAQLIPG
                     AQALPIPRRDHMLAVGDRVYKDGVLAFLARRP"
     gene            1647..1898
                     /locus_tag="VFR00_00080"
     CDS             1647..1898
                     /locus_tag="VFR00_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009463639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HEU0157670.1"
                     /translation="MAVSITPHLANDRGVEKIYIGVREFQCMGARPPHDHPHVFLDMG
                     ADPQILCPYCSTLYIHDGRLAADQSDPPGCLVNPARAEA"
     gene            1920..3095
                     /locus_tag="VFR00_00085"
     CDS             1920..3095
                     /locus_tag="VFR00_00085"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEU0157671.1"
                     /translation="MASARPILVLGGGIAGLALAISLARNGRASMVLESRPQFETEGA
                     GIQLASNGVKVLERLGVAEPLKPLVGAPSCLTVFQGRSGRRLAELPLGTWLAARHGAP
                     YWTMHRADLHAALLAAAAAEPRIVLSAGYALASVRQEAASVCLQDRAGGRLAGEALLG
                     ADGLWSTVRESICAAARPQFAGATAARTLIAAQDAGILAGPYVGLWLGPGANVAHYPV
                     RAGREIAVVIIAREAWPATGADSRVDAAQALRPLAAFHPSLTEVLQRAGDYRKWALYR
                     LPALSAWSKGRIGLLGDAAHPMLPHLAQGGALALEDGLLLGALLGRPSADVGVSLRQF
                     DVQRRQRAARVTAQSRRNGQLYHLAQPLAWGRDAVLRCLPGHWLMAGYDWLYGWRPD"
     gene            3191..4114
                     /locus_tag="VFR00_00090"
     CDS             3191..4114
                     /locus_tag="VFR00_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009763456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEU0157672.1"
                     /translation="MRNGLKRLFSRLYGWPSLLLTLTCMFWAGNTIAGRLAVGQVTPM
                     QLTLLRWVLVIAVLWPIYGQQVRQHWGAVRARLLAVVLMAVFGFTGFNALYYVAAHYT
                     GAINIGILQGCLPIFVLAGAFLAHGTRASLLQVIGALITTLGVVVIATRGAPLSILDV
                     DLNVGDVLMLVACVLYAFFTVALRDRPDMPGAAFFTLLALIAALTSLPLAVWEIFAQA
                     APAPSLTGLLIIAYVAIFPSCLAQIFLLRSVDLIGPGRTGIFLNLVPLFSAIMAVGLL
                     GEPFAPFHAVALVLVLGGILLAQRTPRRDAR"
     gene            4252..>4504
                     /locus_tag="VFR00_00095"
     CDS             4252..>4504
                     /locus_tag="VFR00_00095"
                     /inference="COORDINATES: protein motif:HMM:NF013522.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase family protein"
                     /protein_id="HEU0157673.1"
                     /translation="MPLVRIDIPKGKSPEFRRAISQGIQAALVETFDVPEDDLFQIVS
                     EHAADSPIVHAPSYLGLEYSPELTIIQLTVSDTRSLAQKR"
BASE COUNT          665 a         1515 c         1495 g          829 t
ORIGIN      
        1 tcaccagcgg cccgccggcg ccagcaacct gatgctgggt gacctgtggg gaagcgacgt
       61 gctggccacc acctcgcgcg gggcgggcaa tacgctggcc atggccgaat acgcgctggc
      121 cggcattctg catttcgcca agggcttgca gcgggcagcc gtcgatcgcg aggccggcgc
      181 ctttcagcac cgggcctatc ggcctctgct gctcgagggc aagaccgctc taatcgtcgg
      241 cgccgggggc atcggccttg aggtcggcag gctgtgtgcg gccttgggca tgcgcgtcgt
      301 cggcacgcgg cgcacgcctg ccgccggggc agccttgcca accggcttcc gccgtctcgg
      361 cactcccgcc gagctcgatg gcttcctgag ggaaagcgat gtcgtcgcta tctgctgcca
      421 atggacgccg gagacagacc ggctgttcaa cgcccagcgt tttgccctga tgaagaaggg
      481 aagcattctc gtcaatgtcg cgcgcggaga gatcatcgat gaggcggcgc tgctcgaggc
      541 cttgcagcgc gatcagttgc gcggcgtggt cctcgatgtc tatacggggg agttcgagca
      601 tccgccgccg caaggcctgt ggcgggatcc ccgcgtgctc gtgacgccac atatctccgg
      661 agcgagcgat caggaccggc acggcgctat cgcgattttt tgcgagaact tgcgcgccta
      721 tctcgacggc cagccgctcc gtaatttgat cgactggacg cggggctatt aacgcgacct
      781 cgcccgctag ggccggcggg caagaaacgc cagcacgccg tccttataga cgcgatcgcc
      841 gacagccagc atgtgatcgc ggcgcgggat cggcagcgcc tgggcgccgg gaatgagctg
      901 ggcaagctct gcagccgaac cgccgataac gtcctccgtg ccggtggcca ccagcacggg
      961 gcagctaatc ttggcgacca tctcgcggct gatcggttcg cgggacccgc gaatgcacgc
     1021 ggccagtgcg gcaagatcgc tgccggtctt ttccgcaaag gcgcggaagg tgcgcgcggt
     1081 cggattgacg acctcgtcga tcgacttggc ctcgagtgcg tgcgcgatcg gccccgttcc
     1141 tgccatcggt cggatcatat tgccgccgag cccgccgaaa atggcgctgc gcagacgctc
     1201 ggggtgcgcc agggcgaaga atgcggtgag gcgcgctccc atcgaatagc ccatcacatc
     1261 ggcgcgggcg atgccgaggt gatcgagcag ccggcgggcg tcctccatca tgctggcggg
     1321 ctgataggcc tgggggtcat agagtttcgc gctttcgccg tggcctcggt tgtcgtaggc
     1381 aatcacccgg taggcgttgc gccgcaacag actgacccat cccggatcga tccaattata
     1441 tttgacgctg gaggcgaagc cgtgcacgag cagtaccgga tcgccgctgc cttcgtcgat
     1501 aaagcggatg gaaacgccgt ctgaatcgaa ggcctgcacc gcctgctcct tctcatcgac
     1561 ccactgcgac gttaccaacc cctatcatcc gcagcggtga acaactacac ttgctgcgtt
     1621 cttggatttt gcgcgcaggg ccgcccatgg cagtttcgat caccccgcac cttgccaacg
     1681 accggggcgt ggaaaagatc tatatcggcg ttcgcgagtt ccaatgcatg ggcgcgcgac
     1741 cgccacacga tcacccgcat gtgttcctcg acatgggggc cgatccgcag attttgtgcc
     1801 cctattgttc gaccttgtat atccacgacg gcaggctggc ggccgaccaa tctgacccgc
     1861 cgggctgcct cgtcaacccg gctcgggcgg aagcctgagg cattcgagcg gctgcagcgg
     1921 tggccagtgc gcggccaatt ctggtgctcg gcggcggcat cgccggcctg gccctggcaa
     1981 tctcgttggc ccgcaacggc cgcgccagca tggtgctcga gagccggccg cagtttgaga
     2041 cggaaggcgc gggaattcag ctcgccagca acggggtcaa ggtcctcgag cgcctcggcg
     2101 tggccgagcc actgaaaccg ctggtcggtg cgccgtcctg cctcaccgtg tttcagggcc
     2161 gctcgggacg gcgcctggcc gagctaccgc tcgggacgtg gctggccgcg cgccacggcg
     2221 caccctattg gaccatgcat cgcgccgacc tgcatgcggc gctgctggcg gctgcggcag
     2281 ccgagccgag gattgtgctg agcgcgggtt atgccctcgc cagcgtgcgg caagaggcag
     2341 cctcggtgtg cctccaggac cgtgcgggcg gacgcctcgc cggggaggcg ttgctcggcg
     2401 ccgacggcct gtggtccacc gttcgcgaga gcatttgtgc cgccgcgcga ccgcagtttg
     2461 cgggcgccac cgcggcgcgc acgctcattg cggcgcagga cgccgggatc ttggccgggc
     2521 cctacgtcgg gctttggctc gggcccggcg ccaatgtcgc gcactatccc gtgcgcgcgg
     2581 gacgcgagat tgccgtcgtc atcatcgccc gcgaagcgtg gccggcgacg ggcgctgaca
     2641 gccgtgtcga cgccgcccag gcgctgcgcc cgcttgccgc cttccacccc tcgctcaccg
     2701 aggtgctgca acgggcgggc gactaccgca aatgggcgct ctatcgcctg ccggccctat
     2761 ccgcctggtc gaagggacgc atcggtttgc tcggcgatgc cgcccacccc atgctgccgc
     2821 atctggcgca ggggggcgcg ctggccctgg aagatgggtt gctcttgggg gccctcttgg
     2881 gtcggccaag cgcggacgtc ggcgtcagcc tgcgccagtt cgacgtgcaa cgccgccagc
     2941 gtgccgcccg cgtgaccgct cagagccgcc gcaacggtca gctctaccat ctggcgcagc
     3001 cgctggcgtg gggccgcgac gcggtcttgc ggtgcctgcc gggtcattgg ctgatggccg
     3061 gctacgactg gctctatggt tggcggccgg actgagtgcc acgccgtgcc aagcggcgtg
     3121 cacctgccag ggcgtcggaa ttctgctagg tgtcggcgtc cactggtctc tcccgcacaa
     3181 tcggtctggg atgcgcaacg gcctcaaacg cctgttttca cgcctctacg gctggccaag
     3241 cctgttgctg acgctgacct gtatgttttg ggcgggcaac accatcgccg gtcgcctggc
     3301 ggtcggtcag gtcacaccca tgcagctcac cctcctgcgc tgggtgctcg tgattgccgt
     3361 gttgtggccc atttacgggc aacaggtgcg acagcactgg ggcgccgttc gcgcccgcct
     3421 cctagccgtg gtgctgatgg cggtgttcgg cttcaccggc ttcaacgccc tctattacgt
     3481 ggcggcgcac tacaccgggg ccatcaacat cggcatcctg caaggctgcc tgccgatctt
     3541 cgtgttggcc ggcgcctttc tcgcgcatgg cactcgggca agcctgctgc aggtgatcgg
     3601 cgccctgatc acgacgctcg gcgtggtggt gattgccacc cgcggcgccc ccctatcaat
     3661 cctagacgtc gatctcaatg tgggagacgt cttgatgctg gtcgcctgtg tcctctacgc
     3721 gttcttcacg gtggcgctgc gcgatcgtcc cgacatgccg ggcgcggcct ttttcacgct
     3781 gctggcgcta atcgcggcct tgacctcgct gccgctggcg gtctgggaga tctttgcgca
     3841 agcagcgccg gcgccgagcc taacgggact gttgatcatc gcctacgtcg ccatctttcc
     3901 ctcctgcctg gcgcaaatct tcctgttgcg cagcgtggac ctgatcggac cgggccggac
     3961 cggcatcttt ctgaacttgg tgccgctttt ctccgccatc atggcggtgg ggctgctggg
     4021 tgagccgttc gccccgttcc atgccgtggc gctggtcctc gtgctcggcg gcatcctttt
     4081 ggcccagcgc acgccgcgcc gggacgcgcg ctaggcaggc aagcccgtca gcgcgcgcgt
     4141 gcgcgcgccc catgaaatat tctcacaaga ggggcaaagc attgtcgttt gtcgcagggc
     4201 attggcgccc ccatcttgcc tgccagatca gcctccactg gagatcgcgc tatgccgctc
     4261 gtgcgcattg atataccgaa gggcaaatcg ccggaattcc gccgcgccat ttcgcagggg
     4321 atccaggcgg cgttggtcga aacgttcgac gttccggaag atgacctgtt tcagatcgtg
     4381 agcgagcacg ctgccgacag cccgatcgtg catgcgccga gctaccttgg tcttgaatat
     4441 tcccctgagc tgacgatcat ccagctcacg gtcagcgata cgcgcagcct cgcgcaaaag
     4501 cggc
//
LOCUS       DASTMT010000005         8781 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_2298733, whole genome shotgun sequence.
ACCESSION   DASTMT010000005 DASTMT010000000
VERSION     DASTMT010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 8781)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8781)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8781
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2298733"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..626)
                     /locus_tag="VFR00_00100"
     CDS             complement(<1..626)
                     /locus_tag="VFR00_00100"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008834648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEU0157674.1"
                     /translation="MPASALMNVMIAAARKAGRGLTRDFGEVEQLQVSVKGPGNFVSA
                     ADHRAEDVLFRELGKARPGYGFLMEERGAVAGSDKTHRWIVDPLDGTTNFLHGIPLFC
                     ISIGLEREGEIVCGLVYNPILDELYTAERGQGAFVNNRRLRVANRKTLADSLISTGMP
                     HRGRPGHERFLLECKALMAATGGLRRTGAAAMDLAWVAAGRFDGFVEH"
     gene            894..2294
                     /locus_tag="VFR00_00105"
     CDS             894..2294
                     /locus_tag="VFR00_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002715095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0157675.1"
                     /translation="MAYAAVGLLDSISEFCRRAGMAESTFGRRAVNDGKFVARLRDGA
                     RITPETLERVSAFLTTQGIAAPVSPPELKPLLRVQALAEVGARRPVEDGPSRNFRFFD
                     NRQKYLLFVNTCSEKEVIARRVGMELAHLHPEPPAVRVFDAGMGDGTLLTRLLREMHR
                     RFPTLPFYVVGKEISLEDTRLSLDKMPDRFHEHPATVLVVTNMYYTEAPWLAPNQVSA
                     ATSLAWHEVALTGSTAAEFSEQIAALEPLLARVWQARHSPKTGNPVYERPAAIVIYRE
                     DFRFLLDDVVPRQGRARADYDLVIASQPYRARVPVAFKASRVLSPLVRALRPGGRLLG
                     IHSYGRDPGLEIIRKIWPEEDPFQSSRHDILRATRAELGRDARHYNFNAYADQRAVFR
                     YDMHTLPTEIAASIGTSTLFAAWNAAVYVAQIDDERLGQVLGERKYLDATREVLQTHG
                     RLWFQDESYVISRKRG"
     gene            2301..3206
                     /locus_tag="VFR00_00110"
     CDS             2301..3206
                     /locus_tag="VFR00_00110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0157676.1"
                     /translation="MPASSLDSAPGGSDRSASVAVAGELVACGSLEMGAEAPEDARRI
                     AELLPAGTPVYVNHLPRHELPHTLAALVALRDAGLEPVPHIAARRVASRAQLAGFLDQ
                     AVRVAGVAKALLIAGDVQTPLGPYADGAALIGDGVLADSGLKQVGLPGYPEGHPQIST
                     AVLNAVLEEKLALLEAQRLAGYLVTQFSFAPNRIVEYCADLLRRAPSVPVYVGLAGPT
                     TPRRLIKFAQRCGVSASLRALQAEGMRAVRLFTQVDPSAQLTAIARHARSGSASNVVG
                     VHLYSFGGVAATAAWMNTQITGGQR"
     gene            3407..4243
                     /locus_tag="VFR00_00115"
     CDS             3407..4243
                     /locus_tag="VFR00_00115"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEU0157677.1"
                     /translation="MGKAMRTFLLGGLCLLGAGAPASSEDPRTVPDPRWGAFFAGTDQ
                     RVPIEPTAWPWYAIGRVNIADSVSRRHCTGTLVGAQLGLTAGHCLFDHRLGRWVKPEH
                     VHFVAGQARDSARGHAAAVALIVPPELDMASGSDPAQRTLRLDLVAHDWALVRLEAAL
                     SIKPVPVKAILAGAAAQEVGGSEIARAGYSADRRYLLSVHRGCAAALGQGGTLLNRCD
                     ALPGDSGSPLLLLRGDQAFIIGLSTGAQHMKRADTGYVAVTGLGPSATAFEAAVAKAL
                     AP"
     gene            complement(4259..4627)
                     /locus_tag="VFR00_00120"
     CDS             complement(4259..4627)
                     /locus_tag="VFR00_00120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0157678.1"
                     /translation="MKLSQENARRLSALVWRERLRRLAPIVLAVLVLTGLLTVFLVRQ
                     MDRADRTVSVAVHNATVVTVKQGGGARAAIINVHLDDGRDVEAFSGLRWTPFAGAHVV
                     INEALHASGKHTFDVVRVAE"
     gene            4937..6283
                     /locus_tag="VFR00_00125"
     CDS             4937..6283
                     /locus_tag="VFR00_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012044181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0157679.1"
                     /translation="MRRGRPTRRHFLAGSSAAAATVLSAPYVRRANAAGSLSIGLWDH
                     WVPGANEMQSTIIKEWADAEKVEVKVDYITSQGNKLLLTIAAEAQAKAGHDIMEFTNW
                     EAAQHAFALESTDDVMKQVLERNGPIDPSVEYLGKWKGAWVTVPMTRGSLLMSVCSRY
                     DLLKQHAGIDVQAMYPAGKDAAPEADKWTWENLLIAAEKMQKAGFAFGIPLGVTTDSV
                     LWTGGMFKAFGADLIDATGNVTAKTDAVRKAVDYGKRLAAFLPPDAAAWDDASNNKWL
                     ISGKGSLIFNPPSAWAVAKRDAPQVAEKCWTHGFPKGPAGRFNPMLPRLYGVWNFSKN
                     KAAAKSLLLHLTGRQAAERQVASSQGYDLPPYTKFYDFKTWNENGPPVSTLSHYPIKG
                     GDQKSTIACSPAPPLIAAQIWSQAIMPKMIVRHATGEPMEKTLDWAEAELEGFKRT"
     gene            complement(6396..7988)
                     /locus_tag="VFR00_00130"
     CDS             complement(6396..7988)
                     /locus_tag="VFR00_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011663520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HEU0157680.1"
                     /translation="MAAASAQRNGPHKRAAAPADRIEPHRLLAFLVMVFGMFMAILDI
                     QIVSASLTEIQAGLSASANEITWVQTAYLIAEVVMIPLSGYLSRALGTRILFSVSAAG
                     FTAASLMCGLSSNINEMIFWRAVQGFIGGGMIPTVFATAYIIFPRSKMSLVTPMIGLV
                     ATLAPTVGPTVGGYLTDALSWHWLFFINIIPGLLVTLAALTLIDFDQPDWSLFRNFDW
                     LGLLSMAGFLGALEYVLEEGPRNDWFGDNSVLLMAWVSGLSALVFFARVLLAAQPIVD
                     LRAFADRNFGLGSLFSFVLGIGLYGLTYLYPLYLAQVRGYNALMIGETLFVSGAAMFL
                     TAPVAGRLSTKVDPRLMLMLGFACFAMGTYWLTSLTKDWDFWELFWPQVLRGIGLMLA
                     IIPINNIALGTLAPELVKNASGLYNLMRNLGGAVGLAALTTLLNDRTDLHLSRLHEAV
                     TWSRQPALEALNTLTQRFSSYGSDAQLMAIKQLNAIVHRQGVVMAFADVFAVLTVLFL
                     ALAALAIVMKRPVAAPTGAAAH"
     gene            complement(8010..>8781)
                     /locus_tag="VFR00_00135"
     CDS             complement(8010..>8781)
                     /locus_tag="VFR00_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012496975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HEU0157681.1"
                     /translation="TTAQEAAVDQAKAQLASANAGATRAQLELQRQKDLATRQINSRQ
                     ALEQAEANAEQAVASVQAAQAAIAAASANVDVLKAQQEEARRTLEQYRTALAKAERDL
                     SFTVIKAPFAGVIGNRAVQVGDYVQPTQRLASLVPMAAVFIDANFKETQLAALKPGQP
                     VAISVDALPGRTLEGRVDSLAPASGSVFSLLPPDNATGNFTKIVQRLPVRILLPAEVT
                     EQSLLRPGMSVVVSVNTKPGAPVATQRLGSAAVRTHTN"
BASE COUNT         1532 a         2896 c         2974 g         1379 t
ORIGIN      
        1 tcgtgctcga cgaagccatc gaagcggccg gcggcaaccc aggccaaatc catggcggcg
       61 gcgccggtgc ggcgcaggcc gccggtggcg gccatcaagg ccttgcactc gagcaaaaag
      121 cgctcgtgcc cggggcggcc acggtgcggc atgcccgtgc tgatcaagct gtcggcgagc
      181 gtcttgcggt tggcgacgcg caggcgccgg ttgttgacga aggccccctg tccccgctcg
      241 gcggtataga gctcatcgag gatgggattg taaacgaggc cgcaaacgat ctcgccctcc
      301 cgctcgagcc cgatggaaat gcagaagagg gggatgccgt gcaggaaatt ggtcgtgcca
      361 tcaagaggat cgacgatcca gcgatgcgtc ttgtcggagc cggcgacggc gccgcgctcc
      421 tccatcaaaa agccgtagcc gggacgcgcc ttgcccagtt cgcgaaacag cacgtcttcg
      481 gcgcgatggt cggcggcgct gacgaaattg ccggggcctt tgacggagac ctgcagctgc
      541 tcgacttcgc cgaagtcgcg cgtcaagccc cgcccggctt tgcgggcggc ggcaatcatg
      601 acgttcatca gggctgaggc cggcatcagc ggagggcagg tccaagcggt gtgcgtggaa
      661 ggcccgcccg atctagcaag ggttgcaggg cctgacaagg cgcacgccgg tcgcggccat
      721 ggcgccgacc ggggcggcgg cgccgcaggg gtgggaagtg ggaaacatcc cattctaacg
      781 ctatccgcgt ttgtggttgt gatcccatgg gccagcgcct taggctcaag agccgccatg
      841 gggcggtcga aggcgcgtcc ctcccggcgc ggcccgcagc aggagcccac ctgatggcct
      901 atgccgccgt cggactgctc gacagcatct cggaattctg ccggcgggcc ggcatggccg
      961 aatcgacctt cggtcggcgc gcggtcaatg acggcaagtt cgtggcgcgg ttgcgcgacg
     1021 gcgcccgcat cacccccgaa acgctcgagc gcgtcagcgc ctttctcacc acccagggca
     1081 ttgcggcccc cgtgagcccg cccgagctaa aaccgctgtt gcgcgtgcag gcgctggccg
     1141 aggtgggcgc ccgccggccc gtcgaggatg ggccctcccg gaacttccgc ttcttcgaca
     1201 atcgccaaaa gtatctgttg ttcgtcaata cctgcagcga gaaggaggtg attgcccggc
     1261 gggtcggcat ggaactcgcc cacctgcatc ccgagccacc ggccgtgcgc gtgttcgacg
     1321 ccggcatggg cgatggcacg ctattgaccc gtctcctgcg cgagatgcac cgccgcttcc
     1381 cgacgctgcc gttttacgtg gtcggcaagg agatcagcct cgaggacacg cgcctgtcgc
     1441 tcgacaagat gcccgaccgc tttcacgagc accccgccac ggtgctcgtc gtcaccaaca
     1501 tgtattacac cgaggcgccg tggctggcgc ccaatcaggt ctcggcggcg acgtcgctgg
     1561 cctggcacga ggtggcgctg acgggcagta cggcggccga gttcagcgag cagattgccg
     1621 cgctcgagcc attgctggcg cgcgtctggc aagcgcggca cagtcccaag acgggcaatc
     1681 ccgtgtacga gcgcccggcc gcgatcgtca tttatcgcga ggacttccgc tttctgctcg
     1741 acgacgtcgt gccgcgccag ggacgcgcgc gggccgacta cgacctcgtc atcgcctcgc
     1801 agccctaccg ggcgcgcgtg cccgtcgcct tcaaggcctc gcgcgtgctg agcccgttgg
     1861 tgcgcgccct gcgcccgggt ggccgcttgc tcggcatcca ttcctacggc cgcgacccgg
     1921 ggctcgagat catccgcaag atctggccgg aggaggaccc ttttcagtcc agccggcacg
     1981 acatcttgcg cgccacccgc gccgaactcg gtcgcgatgc gcgccactac aacttcaatg
     2041 cctacgccga ccagcgcgcg gtctttcgct atgacatgca cacgctgccc acggaaattg
     2101 cggcgagcat cggcacctcg acgctgtttg cagcttggaa tgccgccgtc tacgtggcgc
     2161 agatcgacga cgagcgctta ggccaggtgc tgggtgaacg caaatatctc gatgccacgc
     2221 gcgaagtgct gcagacgcac ggccgcctgt ggtttcaaga cgagtcctac gtgatatcac
     2281 gcaagagagg ctagagctcg atgcccgcgt caagtctcga ctcggcgccc ggcggcagcg
     2341 accgcagcgc ctccgtcgcc gtcgccggcg agctcgtcgc ctgcggctcg ctcgagatgg
     2401 gagcggaagc tcccgaggat gcgcgccgta tcgccgagct gttgccggcg ggcacgcccg
     2461 tctacgtcaa tcatttgccg cgccacgaac tgccgcacac gctggcggcg ttggtcgcgc
     2521 tcagggacgc gggcctggag cccgtgccgc acatcgccgc gcgtcgcgtc gcctcgcggg
     2581 ctcagctcgc aggcttcctg gaccaggcgg tgcgcgtggc gggcgtcgcc aaggcgctgc
     2641 tgatcgcggg cgacgtgcag acaccgctcg ggccctatgc cgacggcgcc gccctgatcg
     2701 gggatggcgt gctcgccgat agcggcttaa agcaggtggg attaccgggc tatcccgagg
     2761 gccatccgca gatcagcacg gcggtgctca atgcggtcct cgaggagaag ctggcgttgc
     2821 tcgaagcgca gcgcctcgcc ggttacctgg tgacgcaatt ctccttcgcg ccgaaccgca
     2881 tcgttgagta ctgcgccgat ctcttgcggc gtgcgccctc cgttccggtc tatgtgggat
     2941 tggcgggacc gaccacgccg cgccgcctca tcaagttcgc gcagcgctgc ggggtttcgg
     3001 cctccttgcg cgcgctgcag gcggagggca tgcgggccgt gcgcctcttc acccaggtcg
     3061 atccgagcgc gcaactgacg gccatcgccc ggcatgccag gagcggcagc gccagcaatg
     3121 tggtgggtgt ccacctctat agcttcggcg gggtggcagc gaccgcggcc tggatgaata
     3181 cgcagatcac gggtggccag cgctaggccg gcccgagccc ccgcagcaac gaccaacgcg
     3241 aggcatgccg cgctcggggt ttgccggccc ttggtcgctt gcggcctcgg cctcagacgg
     3301 ccgatgctgg ccgctggcgc cgggcagccg cgctggagcc cggctgccga gacgcttgcc
     3361 ggcagggcgc aaaaactcgc gggcgggcaa caggaggtgg cgggccatgg gcaaggcgat
     3421 gcgcacgttt ctgttgggcg ggctgtgcct cctcggggct ggcgcgccgg ccagcagcga
     3481 ggatccccgc accgtgccgg atccgcggtg gggtgccttc ttcgccggca cggaccagcg
     3541 tgtgccgatc gagccgacgg cttggccctg gtatgcgatc ggccgcgtca atattgccga
     3601 tagcgtcagc cgacgccatt gcaccggcac gctcgtgggc gcccagctgg ggctgacggc
     3661 cgggcactgc ctgttcgatc atcgcttggg acgctgggtt aagcccgagc acgtgcactt
     3721 cgtcgccggg caggcgcgcg acagcgcgcg cggccacgcg gcggccgtgg ccctgatcgt
     3781 gccgcccgag ctcgacatgg cctctggcag cgatcccgcg cagcgaacgc tgcggctcga
     3841 tctcgtcgcc cacgactggg cgctcgtgcg cctggaggcg gcgctgagca tcaagccggt
     3901 tcccgtcaag gcgatcctcg ccggcgcggc ggcgcaagag gtgggtggta gcgagatcgc
     3961 ccgcgccggc tatagcgcgg atcggcgcta tctcttgtcg gtgcatcgcg gctgtgccgc
     4021 agcgcttggc caaggcggca cgctgctgaa ccgttgcgat gctttgccag gcgactccgg
     4081 ctcgccgctg ctgctgttgc gcggggatca ggcctttatc atcgggctca gcaccggcgc
     4141 ccagcacatg aaacgggccg acaccggtta cgtggccgtg acgggtttgg gtccctctgc
     4201 cacggccttc gaggccgccg tcgccaaggc gttggcgccc tgaggccaca cgaggccgct
     4261 actccgccac acgcacaacg tcgaaggtgt gcttgcccga agcgtgcagc gcctcgttga
     4321 tcaccacatg cgcgccggca aaaggcgtcc aacgcaagcc gctgaaggcc tccacgtcgc
     4381 gcccatcgtc gaggtgcacg ttgatgatgg ccgcgcgcgc cccgccgccc tgctttaccg
     4441 tgaccacggt ggcgttatgc accgcgacgc tcaccgtacg gtcggctcga tccatctgcc
     4501 gcaccagaaa cacggtgaga aggccggtca ggaccaacac cgccagaacg atgggagcca
     4561 agcggcgcag ccgctcccgc cataccaagg cggagagacg gcgcgcgttt tcttggctca
     4621 gcttcatcgt ttacaagctc ctccgacggc ccgcgcgcgc cctgaggcct aacgcgcgcg
     4681 ggggcattgc gcaagccggc ggggcccggc cggtgttcgg ctgctcgatc gaaagtctta
     4741 tgccgggtgg cccaagtgtt cataaacctg gggcaaccgg ctcgcggcga gctaaaaacg
     4801 gccgaattga tttgcccaca tcctatctgg gcggcacagt tgcgccatcg gggcggccgc
     4861 accgctaagg cgggcgatcc tagcttgtcg cggcgcgggg ctgccctgtc aacgaaggga
     4921 gaagggagaa atgcccatgc gaagaggaag acctacccgc cgccatttcc tggccgggtc
     4981 gagcgccgcc gccgccaccg tattgtccgc gccctacgtg cgccgggcca atgcggcagg
     5041 cagcctatcg atcgggctct gggatcactg ggtgcctggt gccaacgaga tgcagagcac
     5101 catcatcaag gaatgggccg atgcggaaaa ggtcgaggtg aaagtcgact acatcaccag
     5161 tcagggtaac aagctgctgc tcaccattgc cgccgaggcc caggccaagg ccggccacga
     5221 catcatggag ttcacgaact gggaggcggc tcagcacgcc ttcgcgctcg aatccaccga
     5281 tgatgtcatg aagcaggtgc ttgagcgcaa cggtccgatc gatccgtcgg tggagtatct
     5341 cggcaaatgg aagggcgcct gggtcaccgt gccgatgacc cgcggctcgc tgttgatgtc
     5401 cgtatgcagt cgctatgacc tgctcaagca gcatgccggc atcgacgtgc aggcgatgta
     5461 tcccgccggc aaggatgccg cgccggaagc ggacaaatgg acgtgggaga atttgctcat
     5521 tgccgccgaa aaaatgcaga aggccggttt cgcctttggc atcccactcg gcgtcaccac
     5581 cgacagcgtg ctgtggaccg gcggcatgtt caaggccttc ggcgccgatc tgatcgacgc
     5641 cacgggcaac gtgacagcga agaccgacgc cgtgcgcaag gcggttgact acggcaagcg
     5701 actggcagcc ttcctgccgc ccgacgcggc ggcgtgggac gatgcgtcga acaacaagtg
     5761 gctgatctcc ggcaagggct cgctgatctt caatccgccg tcggcctggg cggtggccaa
     5821 gcgcgacgcg ccgcaggtgg ccgagaagtg ctggacgcac ggcttcccga agggtcctgc
     5881 cggtcgcttc aatccgatgt tgccgcgcct ctacggcgtg tggaacttct ccaagaacaa
     5941 ggcggcggcg aagagcctcc tcctgcactt gacgggccgg caggccgccg agcggcaggt
     6001 ggcctccagc cagggctacg acctgccacc ctacaccaag ttctacgact tcaagacctg
     6061 gaacgagaac gggccgccgg tcagcacgct gtcgcactat cccatcaagg gtggcgacca
     6121 gaagagcacg attgcctgct cgccggcacc acccttgatc gccgcccaaa tctggagcca
     6181 ggcgatcatg cccaagatga tcgtgcgcca cgccacgggc gagccgatgg aaaagacgct
     6241 ggattgggcg gaagccgagc tcgaaggctt caagcgcacc tagctcaggc gcacggcaaa
     6301 acactcaagc gatccggcct tctgctgccg gatcgcttgg gccaagtcct gcagggggtg
     6361 gctaagccgc gctagcgccg aggcgcgggc cctcctcaat gggccgccgc gccagtcggc
     6421 gccgcgaccg ggcgcttcat gacgatggcg agggccgcca gcgccaaaaa caacaccgtc
     6481 agtacggcga acacgtcggc aaaggccatc accacgccct ggcgatgcac gatcgcattc
     6541 aattgtttga tcgccatcag ctgggcatcg ctgccgtagg acgaaaagcg ctgcgtcagc
     6601 gtgttgagcg cctccagcgc cggctgccgc gaccaagtga ccgcctcgtg caagcgcgac
     6661 agatgcagat cggtgcgatc gttgagcagc gtggtcagcg ccgccagccc aacggcgccg
     6721 ccgaggttgc gcatcaggtt gtaaagcccg gaggcattct tgacgagctc cggggcgagc
     6781 gtgcccaggg caatattgtt gatcggaatg atggccagca tcaggccgat gccacgcaga
     6841 acctgcggcc agaacaattc ccagaaatcc cagtccttgg tcagcgacgt cagccagtaa
     6901 gtgcccatgg caaagcaggc gaagccaagc atcagcatga gacggggatc gaccttggtc
     6961 gagagccggc cggccaccgg cgcggtcagg aacatggccg cgcccgagac gaacaaggtc
     7021 tcgccgatca tcagggcatt gtagccgcga acctgggcca ggtagagcgg atagagatag
     7081 gttaggccgt aaagcccaat cccgagcacg aaggaaaaca ggctgccgag gccgaagttg
     7141 cggtcggcaa aggcacgcaa gtcgacgatc ggctgggcgg ccaacagcac gcgcgcgaag
     7201 aagacgagcg cggagaggcc cgagacccag gccatcagca gcacgctgtt gtcgccgaac
     7261 cagtcgttgc gcgggccctc ctcgagcaca tattccagtg ccccaagaaa gccggccatc
     7321 gaaaggaggc ccagccagtc gaaattgcga aacagcgacc aatccggctg atcgaagtcg
     7381 atcaaagtca gcgccgccag cgtgaccagc aggccgggaa taatgttgat aaagaacagc
     7441 cagtgccacg acagggcatc ggtcaaatag ccgccgacgg ttgggccgac cgtgggtgcc
     7501 agcgtcgcca ccaggccgat catcggcgtc acgaggctca tcttcgagcg cgggaagata
     7561 atgtaggcgg tggcgaagac cgtggggatc atgccgccgc cgatgaagcc ttgcaccgca
     7621 cgccagaaga tcatttcatt gatgttggat gacagcccgc acatcaggct ggcggcggta
     7681 aagcccgcgg cggagacgct aaacaaaatg cgcgtgccca gcgcgcgcga gagatagccg
     7741 gagagcggga tcatcaccac ctcggcgatc agataggccg tttgtaccca ggtgatctcg
     7801 ttggcggagg cggaaagccc ggcctggatc tcggtcagcg aggccgagac gatctgaatg
     7861 tcgaggatgg ccatgaacat cccgaacacc atcaccaaga aggcgaggag acggtgcggt
     7921 tcaatgcggt ccgccggcgc tgcagcgcgc ttgtgcggac cgttgcgttg agctgatgct
     7981 gcggccatga ctcggctgcc ccttgcggcc tagttggtgt gcgtgcgcac ggctgcgctg
     8041 cccaggcgct gcgtggcgac cggtgcaccc ggcttggtgt ttacgctgac gacgacggac
     8101 atgccgggac gcagcaggga ctgctcggtg acctccgcgg ggagcaaaat gcgcaccggt
     8161 agacgctgta cgatcttggt aaaattgccg gtcgcattgt ccggcggcag cagcgagaag
     8221 acggagcccg atgccggcgc caggctgtca acgcgcccct ccagcgtgcg gccgggcagg
     8281 gcatcgacgc tgatggcaac gggctgtccg ggtttgagcg cggccagctg cgtctccttg
     8341 aaattcgcgt caatgaagac cgcagccatc ggcaccaggc tggcaagccg ctgcgtcggc
     8401 tgcacgtagt cgcccacctg cacggcgcga ttgccgatga cgccagcgaa gggtgccttg
     8461 atgaccgtga acgacagatc acgctcggcc ttcgccaagg cggtgcgata ctgctcgagg
     8521 gtccgtctgg cctcttcctg ctgcgccttg agcacatcga cgttggcgct ggccgcggca
     8581 atggcagctt gggccgcttg cacggacgct acggcctgct cggcgttcgc ctcggcctgc
     8641 tccaaggctt ggcggctgtt gatctggcgc gtggccagat ccttctggcg ctgcaactcg
     8701 agctgggcgc gggtcgcgcc ggcgttggcc gaggcaagct gcgccttggc ttggtcgacg
     8761 gcggcctcct gggcggtggt c
//
LOCUS       DASTMT010000006         2387 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_2129, whole genome shotgun sequence.
ACCESSION   DASTMT010000006 DASTMT010000000
VERSION     DASTMT010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2387)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2387)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2387
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2129"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..313)
                     /locus_tag="VFR00_00140"
     CDS             complement(<1..313)
                     /locus_tag="VFR00_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HEU0157682.1"
                     /translation="MPHAFRSIVLDQVGSTNAEAFALARAGENGPLWVMAHRQTAGRG
                     RSGRKWASEPGNLHASLLLGLACPPAAVQQLSLLAGVAVIDAIAAAAAGIPIANLRLK
                     WP"
     gene            complement(339..1787)
                     /gene="nuoN"
                     /locus_tag="VFR00_00145"
     CDS             complement(339..1787)
                     /gene="nuoN"
                     /locus_tag="VFR00_00145"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011503277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HEU0157683.1"
                     /translation="MSELALTFQPLIPEIVLVVGALALLLLGVVRPETEREAERICWL
                     ALLVVGVALWRLLVQLPGTQLLFGGGFVVDDFARFMKILTLLGSAGALFLSFDYMRET
                     RSLTFEYPVLVLLASAGMMMMISANDLIALYLGLELQSLALYVVAAIRRDDVRSSEAG
                     LKYFVLGALSSGMLLYGASLIYGFTGSTGFAAIAQASKAVGAAQNIGLVIGLVFLLVG
                     IAFKISAVPFHMWTPDVYEGAPTPVTNFLASAPKVAAVALFMRVSLSAFAGVAPQWQQ
                     VMSALAIASMGLGAFAAIGQRNIKRLLAYSAIGNIGYMLIGLAAASPEGARGTIIYLV
                     VYVAMTLGAFACVLSMRRADGMVEEIDELSGLAQSNLAMATVFALLMFSLAGIPPLAG
                     FFAKFYVFVAAVKEGLWPLAIFGVLASVVGAYYYVRIVKIMFFDAPTEKFLAVPAKAG
                     LIMAVTGLFVLLYVVWPAPLVSSADAAARSLF"
     gene            complement(1841..>2387)
                     /locus_tag="VFR00_00150"
     CDS             complement(1841..>2387)
                     /locus_tag="VFR00_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012112148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HEU0157684.1"
                     /translation="ALNRQGVDGAVVQMISHGFISAALFLCVGVIYDRMHTREIAAYG
                     GLVNNMPKYAVAFMIFTMANVGLPGTSGFIGEFLTLIGVFQVNTWVALFATTGVILSA
                     AYALYLYRRMMFGPLEKRSLRAIADMNTREIVVFAPLVLLVVLFGFYPAPLLDVTAVS
                     VKKLVSNYEASLKAQRADNGR"
BASE COUNT          499 a          770 c          784 g          334 t
ORIGIN      
        1 tgggccactt cagccgcagg ttggcaatgg ggatgccggc ggcagccgca gcgatggcgt
       61 cgatcacggc cacgccggcg agcagggaca gctgctgcac ggcggccggc gggcaggcga
      121 gacccaacag caagctggcg tgcagattgc cgggctcgga ggcccacttg cggccggacc
      181 ggccgcggcc ggccgtttgc cggtgtgcca tgacccacag cggcccattt tcgccggccc
      241 tggccagggc gaatgcctcg gcgttggtcg agccgacctg atcaaggacg atgctgcgga
      301 atgcgtgcgg cactggaccc gatcggcgcc tcgctggcct agaacaggct cctggccgcg
      361 gcatcggccg agctgaccag cggcgccggc caaaccacat agagcagcac gaacagaccc
      421 gtcaccgcca tgatgagacc ggccttggcg ggaactgcca gaaatttctc cgtcggcgca
      481 tcgaagaaca tgatcttgac gatgcgcacg tagtaatagg cacccacgac gctggcaagg
      541 acgccgaaaa tggcaagcgg ccacagcccc tctttcacgg ccgcgacgaa cacgtagaac
      601 ttggcaaaga agccggcgag cgggggtatg ccggccaggg aaaacatcaa cagggcgaat
      661 accgtggcca tggcgaggtt gctctgggcc agtccgctca gctcgtcgat ctcctccacc
      721 atgccgtcgg cgcgccgcat cgacagcacg caggcgaagg cgccgagcgt catggccacg
      781 tagacgacga ggtaaatgat ggtgccgcgc gcgccctccg gtgaggcggc agccagcccg
      841 atcagcatgt agccgatgtt gccgatggcc gagtaggcca gcagccgctt gatgttgcgc
      901 tgaccaatgg cggcaaaggc gccaaggccc atggaggcga tggccagcgc gctcatcacc
      961 tgttgccact ggggcgccac cccggcgaag gcgctcaagc tgacgcgcat gaacagcgcc
     1021 accgccgcca ccttgggtgc ggaggccaga aaattcgtga ccggtgtcgg cgcaccctcg
     1081 tagacgtccg gcgtccacat gtggaaaggc acggccgaaa tcttgaaggc gatgccgacc
     1141 aggagaaaga ccaagccgat gacgaggccg atgttttgtg cggcacccac cgccttgctg
     1201 gcctgagcga tcgccgcaaa gccggtcgaa ccggtgaagc cgtagatcag cgaggcgcca
     1261 tagagcaaca tgccggagga gagcgcaccc agcacgaagt acttgagccc ggcctcgctc
     1321 gagcgcacgt cgtcgcgccg gatggcggca accacgtaga gcgccaggct ctgcagctcg
     1381 agccccaggt agagcgcgat cagatcgttg gccgagatca tcatcatcat gccagcgcta
     1441 gccagcagca cgagcacggg atattcgaag gtcagcgatc gggtctcgcg catgtagtcg
     1501 aaggacagga acaaggcgcc ggccgagccg agcaaggtca aaatcttcat aaagcgggca
     1561 aagtcatcga cgacgaaacc gccgccgaac agcagctgcg tgcccggcag ctgcaccagc
     1621 aaccgccaca gggccacccc caccaccagc agcgccagcc agcagatacg ctccgcctcg
     1681 cgctcggtct ccggccgcac cacgccgagc agcaggagcg ccaaggcccc gacgacaagc
     1741 acgatctccg ggatcagcgg ctgaaaggtg agggcaagct cgctcatggt cttacgtttt
     1801 acgtgctccc tgcggccgcc tgctgccggc gtcgcttttg ttagcgtcca ttgtcggccc
     1861 gttgcgcctt cagcgacgcc tcatagttgg agacgagctt cttgaccgac accgccgtga
     1921 cgtcgagcag cggcgctgga tagaagccga acagcacaac cagcaacacc aggggtgcga
     1981 acacgacgat ctcgcgggta ttcatgtcgg cgatggccct gaggctgcgc ttctccaggg
     2041 gcccaaacat catgcggcga taaagataaa gcgcgtaggc cgccgacagg atgacgccgg
     2101 tggtggcgaa gagcgcgacc caggtgttga cctggaagac accaatcagg gtcaggaact
     2161 cgcctatgaa gcccgaggtg cccggcaggc cgacattcgc catcgtgaag atcatgaagg
     2221 cgacggcgta tttcggcatg ttgttgacga ggccgccata ggccgcgatc tcgcgcgtgt
     2281 gcatgcggtc gtagatcacg ccgacgcaaa ggaagagcgc ggccgagatg aagccgtggc
     2341 tgatcatctg caccacggcg ccgtcgacgc cttgccggtt gagcgcg
//
LOCUS       DASTMT010000007         3198 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_2379, whole genome shotgun sequence.
ACCESSION   DASTMT010000007 DASTMT010000000
VERSION     DASTMT010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3198)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3198
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2379"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(419..2341)
                     /gene="dnaG"
                     /locus_tag="VFR00_00155"
     CDS             complement(419..2341)
                     /gene="dnaG"
                     /locus_tag="VFR00_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEU0157685.1"
                     /translation="MRFPPSLIDEIRARLPVSQVVARKVALKRAGRELKGLSPFKQEK
                     TPSFFVNDHKGSWFDFSSGQNGDIFKFIMLTEGLSFPEAVERLAEEAGIALPKASRAE
                     AEAEDERARLYQVLEASCRFFESQLGAGAGLEARRYLEGRGLGREASARFRLGYAPNS
                     RSALKEHLAKAGFTGAEMVAAGMQISGEDIPVAYDRFRHRVTFPISDLKGRIIAFGAR
                     ALDPDTPAKYLNSPDTPLFHKGAVLFNAANARRAAFDKARIIVVEGYMDVIALAEAGF
                     QETVAPLGTALTENQLQVLWRLAPSPVLCFDGDAAGRNAAFRAVETVLPHLKPGHSVE
                     FAFLPDGFDPDELVRQHGAGAMTEVLTKTRPLFDVLIDREERRGETATPEQRAAFDDR
                     LKALVQRIADRGVREHYERELRAILWARTAKLTRAIAGRDGRASAFTAKRRNNTQLDW
                     RVAARASERSRLGAAPRAAQLGQAPVRSNELAERSLPVPAREALLVTALLNHPWLLER
                     HCEEVAQLPLTAPPLARLRDGMLALLSQGTPLDALHLRTQLSAIGLNGSVAIAERGIT
                     HKGDKFAEPETDAADVEAGWLHALAMHKSQTELKRHLEGLERAYAAEPSEMAFGQIVE
                     IQQQLLKAHQTLEGQD"
     gene            complement(2510..2962)
                     /locus_tag="VFR00_00160"
     CDS             complement(2510..2962)
                     /locus_tag="VFR00_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003581063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HEU0157686.1"
                     /translation="MRDRLAQATKAALKAQDRGRLSALRLISAALQEREIAQRTEALS
                     EADTLAVLQKMLKQRRDAAAIYQGAGRQDLADQEAKEITVIEEFLPQQMAEGDVRSAI
                     AAVIKDVGATSPREMGKVMAALKSRYAGQMDFGKASALVKELLAGASP"
     gene            complement(3037..>3198)
                     /locus_tag="VFR00_00165"
     CDS             complement(3037..>3198)
                     /locus_tag="VFR00_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007602189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEU0157687.1"
                     /translation="FRLLSEAGLLSFFSTTTVFGTPLDITLSELALEAFFPADAATAD
                     RLGQLAGRA"
BASE COUNT          521 a         1036 c         1090 g          551 t
ORIGIN      
        1 tgggcacttg gtcttcggac ttctgcatcg ccatgtagag ggctccgggg ccgcgcttgc
       61 cgtggatgcg cacgcatcgc tagttcaaca aaactgtgtg gtggcttgcg tgggtgcgcc
      121 aatgcgcaac ggcaaaccac cccgatcagg cgggctatcc cgccgggtga aaacctgtcg
      181 atgaaaccca cggcggcaga tgtgccaggg ccggagcggt gctcgggttg gcctcaatgt
      241 gggaatcgaa gcaggcatac acaacttccg ccgcatttcc taccgtttgt ttctgtggat
      301 gtccttccta ccctaggggg gtttcccaca gcttatgctc tggcgaaaaa aagccctgcg
      361 acctgagcga aacgcaaaaa agggggagcg gtcgcaggtc aggagctcta aactgcgttc
      421 agtcctgccc ctccagtgtt tggtgggcct tgagcagctg ttgctggatc tccacaattt
      481 gtccaaaagc catctcggag ggctcggcgg cgtaggcccg ctccaagccc tcaaggtggc
      541 gcttcagctc ggtttgggat ttgtgcatgg cgagcgcatg caaccatccg gcctcaacgt
      601 cggcggcatc tgtttccggt tcggcaaact tgtcgccctt gtgggtaatg ccgcgctcgg
      661 cgatggcaac actgccgttg agcccgatgg cgctcagttg ggtgcgtagg tgcagcgcgt
      721 caagaggggt gccctggctg aggagcgcga gcatgccatc gcgcaggcgg gccaagggcg
      781 gggccgtcaa gggtagctgg gccacctctt cgcaatgccg ctcgagcagc cagggatggt
      841 tcagcaaggc cgtgaccagg agcgcctcac gcgcgggcac cggcaagctg cgctcggcca
      901 attcgttgct gcgcacgggg gcctggccga gctgagcggc gcgcggcgcc gcgcccaggc
      961 gcgagcgttc gctggcacgg gcggcgaccc gccagtcgag ctgggtattg ttgcggcgct
     1021 tggccgtaaa agcgctggcg cggccgtcgc gaccggcgat ggcacgcgtg agcttggccg
     1081 ttctggccca caggatggcg cgcagctcac gctcgtagtg ttcgcgcacg ccgcggtcgg
     1141 cgatgcgttg caccaaggcc ttgagccggt cgtcaaaggc ggcacgttgt tccggcgtgg
     1201 cggtctcccc gcgacgctcc tcgcggtcga tcaagacatc gaacaggggc cgggtcttcg
     1261 ttaatacctc cgtcatcgcc ccggcgccgt gctggcgcac gagctcgtcg ggatcaaagc
     1321 cgtcgggtag gaaggcaaac tcgacgctgt gtccgggttt caagtgcggc agtacggttt
     1381 cgacggcacg aaaggcggca ttgcgaccgg ccgcatcgcc gtcgaagcac agcaccggcg
     1441 agggggccag gcgccacagc acctgcagct gattttcggt cagcgcggtg ccgagcgggg
     1501 cgacggtctc ctgaaagccc gcctcggcga gggcgatcac gtccatgtag ccctcgacca
     1561 cgattatgcg ggccttgtcg aaggcggcgc ggcgcgcgtt ggcggcattg aagagcaccg
     1621 cgcctttgtg gaagagcggc gtatcgggag agttgagata tttcgcgggt gtgtccgggt
     1681 ccagcgcccg cgcgccgaag gcaatgatgc ggcctttgag atcgctgatc ggaaacgtca
     1741 cacggtgtcg gaagcggtca taggccacgg gaatgtcctc gccgctgatc tgcatgcccg
     1801 cggcgaccat ctccgctccg gtaaagcccg ccttggccaa atgctccttc agcgccgagc
     1861 ggctgttggg ggcgtagcca agccggaaac gggcgcttgc ctcgcgtccg agcccgcggc
     1921 cctcgagata gcggcgcgcc tccaagccgg cgccggcacc cagctggctc tcgaaaaagc
     1981 ggcaggaggc ctccagcacc tgatagagcc gagcgcgctc gtcttccgcc tcggcctcgg
     2041 cgcgggaggc tttgggcagc gcgatgccgg cctcttcggc cagccgttcc accgcttcgg
     2101 ggaagctcaa gccctcggtc agcatgatga acttgaaaat gtcgccgttc tggcccgagg
     2161 agaaatcaaa ccacgagccc ttgtggtcat tgacgaaaaa cgacggcgtc ttctcttgct
     2221 tgaagggcga cagccctttg agctcgcgac ccgcgcgctt gagcgcgacc ttgcgcgcca
     2281 ccacctggct caccggcagc cgggcacgga tctcgtcgat caaggagggc gggaaacgca
     2341 tgggcggtcc aggctcacaa gcgagtgccc gccccacata cgtgattcgc gcgcaagctc
     2401 cttgggcgtc gaggaaaagc gccgctcggt gcccccgcta tccacagccg cgcggcggct
     2461 tgcgcgcagc gcctgcgcgc agcgcccccc gcccgggcga acggacgcct taggggcttg
     2521 cgccggcgag gagttccttc accagcgcgc tcgccttgcc gaagtccatc tgcccggcgt
     2581 agcgcgactt cagcgctgcc atcaccttgc ccatctcgcg cgggctggtg gcacctacat
     2641 ctttgatcac cgcggcgatc gccgacctga cgtcgccctc cgccatttgc tggggcagaa
     2701 actcctcgat gacggtgatt tccttggctt cctggtcggc caggtcctgg cggccggcgc
     2761 cttggtagat ggcagctgcg tcgcggcgct gtttcagcat cttctgcaac acggcgagcg
     2821 tgtcggcctc cgaaagcgcc tccgtgcgct gggcgatctc gcgctcctgc agggcggcgc
     2881 tgatgaggcg cagcgccgag agccgccctc ggtcctgcgc cttgagggct gccttggtgg
     2941 cctgggcgag acgatcgcgc atggggaagc tccgcggcta cgctaacagc ccatgtatgg
     3001 tagcaccgct gggcgcgcaa ggcgggtgca gccggttcat gccctgccgg caagctgacc
     3061 gagccggtcc gcggtcgccg cgtcggcggg aaaaaaggct tccagcgcca gctccgacag
     3121 cgtgatgtcg aggggcgtgc cgaacaccgt cgtggtcgag aaaaacgaca acaggccggc
     3181 ctcgctcagc agccggaa
//
LOCUS       DASTMT010000008         3984 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_1315416, whole genome shotgun sequence.
ACCESSION   DASTMT010000008 DASTMT010000000
VERSION     DASTMT010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3984)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3984)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3984
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1315416"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..359
                     /locus_tag="VFR00_00170"
     CDS             <1..359
                     /locus_tag="VFR00_00170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0157688.1"
                     /translation="SAMFYAGAAQEKRWAAIDPNNETTSPVVWSNTEAKARAEAVEAC
                     KRSSKSCANGPASTSEMEDFFAVMCCTKPRRGCAVGVAGARQDALKNVQKIFADAGYS
                     TCSLRHYLSAGSGKKD"
     gene            435..1481
                     /locus_tag="VFR00_00175"
     CDS             435..1481
                     /locus_tag="VFR00_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEU0157689.1"
                     /translation="MPAEGSSGVLPIETDALIIGAGPVGLFAVFELGLVDIKAHVVDI
                     LDKIGGQCAELYPEKPIYDIPGLPIVTGQGLTDALLAQIKPFGAQFHLNRRIDTIART
                     EDGRFRLGTDAGEQFLAKVVVIAAGGGSFTPKRPPLPGIEAHEGSSVFYSVRRMEDFR
                     DKDVLIVGGGDSALDWTLNLQPLSRSLTLLHRRDEFRAAPHSVEQMRALVAANKIKLL
                     IGQMVALHEEAGALEAVSVRTKEGETRVAANRLMPFFGLTMKLGPVAHWGLNLHENLI
                     RVDTEKFETSEPGIFAVGDINSYPGKLKLILSGFHEAALMAQAAYKIVYPGKKLLFQY
                     TTSSSSLQRKLGVS"
     gene            complement(1446..2729)
                     /locus_tag="VFR00_00180"
     CDS             complement(1446..2729)
                     /locus_tag="VFR00_00180"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU0157690.1"
                     /translation="MSALMSLRAGGWPAAAGAREAALTLPPAFAALTTFAIVADRAGF
                     DALEPEWNDLFARAGRDTHVFQTFNWNWHWANHYLSAAPKAAKPALAIVTGRRRGRLV
                     LLWPLVIARHGGLSVVRWMGEPVSQYGDVLIEDAPDKAAVLMQSWRFLASELGATAIS
                     LRKVRADANVAPLLASLGLEQVDGAVAPYLDLASAPDFAAYERRYSQKARKNRRRLLR
                     RLEEQGAVVVTRHAGGSEARDAARAAIALKRAWTHTTARLAPALADPRFGAFFADVAD
                     GGSRPAGCAVTMLRCQDAVAGIAIDVTCRGHRAAHIIVHDPALKHLSPGTLLLREWIR
                     AASAERIATFDLLAPAYAYKGDWADASMPVSDYAGGFSWRGSLYARLYLGWLRPRLKG
                     ALEALPRLTREWERWRQSGKAQAASADPELPLQGG"
     gene            complement(2830..3195)
                     /locus_tag="VFR00_00185"
     CDS             complement(2830..3195)
                     /locus_tag="VFR00_00185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0157691.1"
                     /translation="MVEQLLWVEMLCKLAAGCALLMAPTTTAAVMGWPRPGAAFWPRL
                     LGALLIGIGLASALDARAAGGRGLGLGGSVSINLAVATFLLVSVMLRPPAARRGRLLS
                     WLIVGCLFLLSLIEIALAG"
     gene            complement(3247..3792)
                     /locus_tag="VFR00_00190"
     CDS             complement(3247..3792)
                     /locus_tag="VFR00_00190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0157692.1"
                     /translation="MHRFARILTAGLLLAGAGLAAGCGGNVAGLTTSSTAPADSKIPA
                     NDDPMARPTFVAWTAARAKRCGFFFDAAKLRAAYLAYESRQGQGPANLANIEKVYDQT
                     FKSTSDSAWAQDDYCSERKGADIKQELARHLAGDYAPNFPKPKVVADCGIFGCSAASE
                     EPFDSKKFWQKNDADKISGRR"
BASE COUNT          684 a         1314 c         1334 g          652 t
ORIGIN      
        1 tcagtgccat gttctacgca ggcgcggccc aggagaaacg ctgggctgcg attgacccca
       61 ataatgaaac aacttctccc gtcgtctggt cgaacacgga ggccaaggca cgcgcggagg
      121 cggtggaggc ctgcaaacgc agttccaaga gctgcgccaa cggcccggcc tccaccagcg
      181 aaatggagga tttttttgcc gttatgtgct gcacaaagcc gcgccggggt tgcgcggtgg
      241 gtgtcgccgg cgctaggcag gacgcgctga aaaacgtgca gaagattttt gctgatgcgg
      301 ggtactcgac ctgttcgctg cgtcactatc tctcggccgg cagcggcaaa aaggactaaa
      361 aggcgtttca gcgtcgagcc ggcgcgttgg ccggttttgg gcagcgtgcg cgcaaccatc
      421 gggatttgaa gtctatgcca gctgagggct caagcggcgt cctgccgatc gagaccgatg
      481 ccctgatcat cggcgctgga ccagttggtc tgttcgcggt gttcgaactg ggcctcgtcg
      541 acatcaaggc gcatgtggtc gacattctcg ataaaatcgg cggccaatgc gccgagctct
      601 atccggaaaa gccgatctac gacattcccg gcttgccgat cgtgaccggg cagggtttga
      661 cggatgccct gctcgcgcag atcaagccgt tcggcgccca gttccatctc aatcggcgca
      721 tcgacaccat cgcgcgcacc gaggacggcc gctttcgtct cggcacggac gcgggcgagc
      781 agtttctcgc caaggtcgtg gtcatcgccg cggggggcgg ctccttcaca ccgaagcgcc
      841 cacccttgcc cggcatcgag gcgcacgagg gcagctcggt attttattcc gtgcgccgca
      901 tggaggactt tcgcgacaag gatgtgctga ttgtgggcgg cggcgattcg gcgctcgatt
      961 ggacgctcaa cctgcagccg ctgtcgcgta gcctgaccct gctgcaccgg cgcgacgagt
     1021 tccgggccgc gccgcattcg gtcgagcaga tgcgcgcgct cgtggccgcc aacaagatca
     1081 aactgctgat cggccagatg gtagcgctgc acgaggaagc cggcgcgctg gaggcggtca
     1141 gcgtacgcac caaggagggt gagacccgcg tcgccgccaa ccgcttgatg cccttcttcg
     1201 ggctgaccat gaagctcggg ccggtggcgc actggggtct caatctgcac gagaacctca
     1261 ttcgggtcga caccgagaaa ttcgagacca gcgagcccgg catttttgcc gtcggcgaca
     1321 tcaattcgta cccgggcaaa ctcaagctga tcctgtcggg cttccacgaa gcggccttga
     1381 tggcgcaggc ggcctacaag atcgtctacc ccggcaagaa attgctgttc cagtacacga
     1441 cctcctcatc ctccctgcaa aggaagctcg gggtcagctg aggcggcctg cgccttgcca
     1501 ctttggcgcc aacgctccca ttccctagtg aggcgaggca aagcctcgag cgcccctttc
     1561 aggcgcgggc gcagccagcc gagatagagc cgggcgtaga gcgagccccg ccagctgaaa
     1621 ccgccggcat agtcgctcac gggcatgctg gcgtcggccc agtccccctt ataggcatag
     1681 gcgggagcca gaagatcgaa ggtggcgatg cgctcggcgc tggccgcgcg gatccactcc
     1741 cgcaacagga gcgtgcccgg gctcaaatgc ttgagagcgg gatcgtgcac gataatgtgg
     1801 gctgcgcgat gcccgcggca ggtgacgtcg atggcgatgc cggcaacggc atcctggcag
     1861 cgcagcatcg tcaccgcaca gccggccggc cggctgccgc cgtccgcaac gtcggcgaaa
     1921 aaagcgccaa agcgcggatc cgccagcgcc ggggcaagcc gagccgtcgt gtgggtccag
     1981 gcgcgtttga gcgcgatcgc cgcgcgggcg gcgtcgcgag cctcgctgcc gccggcatgg
     2041 cgcgtaacga cgacggctcc ctgctcctcg agccgacgca gcagccgccg ccggttcttg
     2101 cgcgcctttt ggctgtagcg gcgctcgtag gcggcaaaat cgggggcgct ggccagatcg
     2161 agatagggcg ccaccgcgcc gtctacctgc tcgaggccga ggctggcgag gaggggcgca
     2221 acgttcgcat cggcgcgcac cttgcgcaaa ctgatggccg tggcgcccag ctcgctcgcc
     2281 aggaagcgcc aggactgcat gagcacggca gctttgtccg gcgcatcctc gatcaggacg
     2341 tcgccatact ggctgaccgg ttcccccatc cagcgcacca cgctcaggcc gccgtggcgg
     2401 gcgattacca gcggccagag caggacgagc cggccgcggc ggcggccggt gacgatggcg
     2461 agcgccggtt tggcggcctt tggtgcggcg ctcagatagt gattggccca gtgccaattc
     2521 caattgaaag tctggaacac gtgcgtgtca cggccggcgc gcgcgaaaag atcgttccat
     2581 tccggctcga gggcgtcgaa gccggcacgg tccgcgacga tggcgaaggt ggtgagcgcc
     2641 gcgaacgcgg gcggcagggt taatgctgct tcgcgcgcgc cggccgccgc cggccagccg
     2701 cccgcgcgca ggctcatcag tgcgctcacc ttgccctcct cctcacgctg ctctgcccgc
     2761 cggccctcgc cgcaggcctc cgttccgagc caatgccgca aggaccgtgc caggggcgtt
     2821 gccggcgcgt cagccggcga gcgcgatctc gatcaggctc aacaggaaaa ggcagcctac
     2881 gatgagccag gagaggagcc ggccgcgccg cgccgccggt gggcgcaaca tcacgctgac
     2941 gaggagaaaa gtagcaacgg caaggttaat gctgacactg ccgccgagcc ccaggccgcg
     3001 gccgccggcc gccctcgcat cgagggccga ggccaggccg atgccgatca gaagggcgcc
     3061 caacaggcgc ggccaaaagg ccgcccccgg gcgtggccag cccatgaccg ccgccgtcgt
     3121 cgttggtgcc atcagcaagg cgcagccggc cgcgagcttg cacagcatct cgacccagag
     3181 cagctgctcc accattgctg cggatggttc cgaggcgcgc ttgccgggga ggcgccgctc
     3241 aagcccttag cgccggccgc tgatcttgtc ggcgtcgttc ttctgccaga acttcttgct
     3301 gtcgaagggc tcctcggagg ccgccgagca gccaaagatg ccgcagtcgg cgaccacctt
     3361 cggtttgggg aaattcggcg catagtcgcc ggccaaatgc cgagccagct cttgcttgat
     3421 gtcggcaccc ttgcgctccg agcagtagtc atcctgcgcc caggccgagt cggaggtcga
     3481 cttgaaggtt tggtcgtaga ccttttcgat gttggcgaga ttggccgggc cttggccttg
     3541 cctgctctca taggcgaggt aggcggccct gagtttggcc gcgtcgaaaa agaagccgca
     3601 gcgcttggcg cgcgccgcgg tccacgccac gaaggtgggc cgcgccatcg gatcgtcgtt
     3661 ggcggggatc ttcgaatcgg ccggcgcggt gctgctggtg gtgagaccgg cgacattgcc
     3721 accgcagccg gcggcaagcc ccgcccctgc gagcagcagt cctgccgtca ggatgcgcgc
     3781 gaatcgatgc attggtcgtc ccccgttccc gcacctactg tgccaagaaa ccgagccgcg
     3841 tcaatcggca cctcatttcg ggcgatatca gggcttaatc gcggcgcccg gcgctttagc
     3901 cgatcgatgg cgctttagcg catcaattct cgcatgcccc gatcgagccc gtcgagcgtc
     3961 aagggataca tgcgctcctc gatg
//
LOCUS       DASTMT010000009         2118 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_5908202, whole genome shotgun sequence.
ACCESSION   DASTMT010000009 DASTMT010000000
VERSION     DASTMT010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2118)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2118)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2118
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5908202"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..194
                     /locus_tag="VFR00_00195"
     CDS             <1..194
                     /locus_tag="VFR00_00195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0157693.1"
                     /translation="EKRFIKTFTAAGAKYHRFSYQDYLEWLRFAQQTAWKEYFALSPT
                     TESLLLEVVQTLMDEAATK"
     gene            complement(235..>2118)
                     /locus_tag="VFR00_00200"
     CDS             complement(235..>2118)
                     /locus_tag="VFR00_00200"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HEU0157694.1"
                     /translation="DDALKGTLRQNTDPLRLHALALSHAEATHPGAREAAFEIAQWAL
                     QSEAAEALAQMSARLAKGEGPLVTLVRKRQDLLAQRRGADKRLLAAVGAADANGAAAL
                     RASIVSYDADMQSIDAELAQKFPDYANFANPKPLTIAEVQALLGPEEALILFLDVKQL
                     GRLPEQTLVWALTRKTASWHRMPLGPRALADAVLALRCGLDGSSWQDGTDKCGQLLAE
                     LEPQATESGQGAGMLPFSLTRAHELYAHLFGPIQHVIGGKHLIIVPSGPLLSLPFSVL
                     VTEPPKTNPSEGLAAYRRAAWLGTRQAISMLPAVASLAALRRHAKRSAAPDPFLGYGD
                     PTLVGVPGCPRVPIPEACPDEKSARAPRLAVASRSAKAARNIARDLAEGGATLSEVRK
                     LCPLPDTAHELKCVARSLGAKADAIVLGKSMTETAVKSAALSRYRVVHFATHGLLAGE
                     VAQLGAARAEPALVLSPPEQPTEDDDGLLTASEVAGLKLDADWVVLSACNTAAGEEVG
                     GEALSGLARAFFYAGARALLVSHWPVDSYAATMLTSHTFAELRKSAGLGRAEAFRRAM
                     LALLSDDRRPWAAHPSIWAPFAVVGSGAEGAMPRAAAAAKARAAKAPLGPEEDWSKRA
                     LAP"
BASE COUNT          350 a          718 c          718 g          332 t
ORIGIN      
        1 ccgaaaaacg cttcatcaag acgttcactg ccgcgggcgc caagtatcac aggttcagct
       61 atcaggacta tctcgagtgg ttgcgttttg cccagcagac cgcgtggaag gaatattttg
      121 ccttgagccc gacgaccgag agcctgttgc tcgaggtcgt gcagaccctg atggatgagg
      181 ccgccaccaa gtagggagtc ccctggcgcc atgacccggt gagaggtcaa cggctcaggg
      241 ggcaagcgcg cgcttggacc aatcctcctc tgggccgagt ggggctttgg cggcgcgcgc
      301 tttcgcggcc gcggccgcgc gcggcatcgc cccctctgcg ccgctgccaa cgaccgcgaa
      361 gggtgcccaa atcgaaggat gggcggccca gggacgccgg tcgtcgctca gcagagccag
      421 catggcccgg cgaaacgcct cggcgcggcc gagcccggcg gattttcgca actcggcgaa
      481 ggtgtggctc gtgagcatgg tcgccgcata ggaatcaacg ggccaatgcg agaccaacag
      541 cgcgcgcgcc cccgcataga agaaggcgcg cgcgagcccc gacagcgcct cgccgccgac
      601 ttcctctcca gccgcggtat tgcaggcgga cagcacgacc caatcggcat cgagcttgag
      661 tccggcgacc tccgaggcgg tgagcaggcc atcgtcgtcc tccgttggct gctccggcgg
      721 cgacaggacc agcgccggct cggcgcgcgc cgcgccgagc tgggccacct cgcctgcgag
      781 gagaccatgc gtggcaaaat gcaccacacg ataacggctg agcgcggcgc ttttgaccgc
      841 cgtctcggtc atgctcttgc caagcacgat cgcatcggcc ttggccccaa ggctgcgggc
      901 cacgcatttg agctcgtgcg cggtatcggg cagaggacag agtttgcgca cctccgagag
      961 ggtggcaccg ccctcggcga ggtcacgggc gatgttcctg gcagcttttg cgctccggct
     1021 ggcgactgca agccttggcg cacgcgcgga cttctcgtct ggacaggcct ccgggatggg
     1081 cacccgcggg caacccggga cgccaacgag ggtcggatcc ccatagccca ggaatggatc
     1141 gggcgccgcg gagcgcttgg cgtgccgacg cagcgccgcc agtgacgcaa cggcgggaag
     1201 catgctgatg gcctggcgcg tgccgagcca ggcggcccga cgataggcgg caagtccctc
     1261 gctcgggttt gtcttgggcg gctcggtcac gagcacgctg aagggcaggc tcagcaatgg
     1321 cccggagggc acgatgatga gatgcttgcc cccgatgacg tgttggatcg ggccgaacaa
     1381 gtgagcgtag agctcgtgcg cccgcgtcag gctgaagggc aacatgcccg caccttggcc
     1441 gctctcagtg gcctgcggct cgagctccgc caagagttgg ccgcacttgt cggtgccgtc
     1501 ctgccagctc gacccatcca aaccgcagcg caacgccagc acggcatcgg cgagcgcgcg
     1561 cggaccaaga ggcatgcggt gccaacttgc tgtcttgcgc gtcagcgccc agaccagcgt
     1621 ttgctccggt aatcgaccca gctgcttgac gtcgagaaac aggatcaggg cctcctcggg
     1681 ccccagcagc gcctgcacct ccgcaatcgt cagcggtttg gggttggcga aattcgcgta
     1741 atcggggaat ttttgcgcca gctcagcatc gatcgactgc atgtccgcat cgtagctgac
     1801 gatcgaggca cgcagcgccg ccgcaccgtt ggcatcggcc gcgccgaccg ccgccagcag
     1861 ccgtttgtcc gcgccccggc gttgcgccag caggtcctgg cgcttgcgca ccagggtgac
     1921 cagcggccct tcgcccttgg cgagccgtgc cgacatttgc gccaaggcct cggcggcctc
     1981 cgactggagc gcccactggg cgatctcgaa ggctgcttcg cgcgctccgg gatgcgtagc
     2041 ctcggcatgg ctcagcgcca gcgcgtgcag gcgtagcgga tcggtattct gccgcaaggt
     2101 gcccttcagc gcatcgtc
//
LOCUS       DASTMT010000010         2724 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_4596070, whole genome shotgun sequence.
ACCESSION   DASTMT010000010 DASTMT010000000
VERSION     DASTMT010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2724)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2724)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2724
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4596070"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..291)
                     /locus_tag="VFR00_00205"
     CDS             complement(<1..291)
                     /locus_tag="VFR00_00205"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEU0157695.1"
                     /translation="MTAAELRRCPWVGIADPEYARYHDEEWGVPKSEDRALFEKLVLE
                     GFQSGLSWLTILKKRENFRTAFHAFDAERIARYGAKDVARLMRDAGIVRNRLK"
     gene            complement(288..1187)
                     /locus_tag="VFR00_00210"
     CDS             complement(288..1187)
                     /locus_tag="VFR00_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009764800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate-binding protein"
                     /protein_id="HEU0157696.1"
                     /translation="MRSRNLALLAERGIVRVAGADAKKLLQGLITNDMDEIGPGAAVH
                     AGLLSPQGKILFEFFVVEAEAAFLLETAAAEAANLSKRLSLYKLRANVEIGEVSVPSA
                     VFALWGEGVIGPVALPGATVFVDPRLPQLGVRIIAPPAAEAALAAATGARPARQDDWH
                     RHRIALGVPEAGRDYRLGDTFLHEANYDQLNGVSFSKGCFIGQEVASRMQHRGGGRKR
                     VVQVEGAAALAPGAPIMAGAVSLGAIGSVDGSRGLALVRLDRAAEALAKGVVLSAEAV
                     PIRLSKPAWARFEMTPSTRTEVL"
     gene            1186..1320
                     /locus_tag="VFR00_00215"
     CDS             1186..1320
                     /locus_tag="VFR00_00215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0157697.1"
                     /translation="MENVLFVAAKGPPLPDLQATGKRVGCNRQASERGRHGAGDRHGH
                     "
     gene            complement(1420..2166)
                     /locus_tag="VFR00_00220"
     CDS             complement(1420..2166)
                     /locus_tag="VFR00_00220"
                     /EC_number="2.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013948584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0018104 - peptidoglycan-protein
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEU0157698.1"
                     /translation="MWRLGLLGFGFLLLAPQAQAQSWSWRSDRAYSYDRPQPQYGLFN
                     WWDDDDDGFYRPRQRGPAQQSGGARPAISPQAPPSLNFPSSYPVGSVVIDTRSRRLFL
                     VQSPTTALVYQISVGREGFTWTGTEKISRKAEWPDWYPPEEMRERDGRLPEKMTGGIK
                     NPLGAMALYLGNSLYRIHGTNDAKSIGQAASSGCFRMLNAHIVDLAGRVEVGTPVTVV
                     HRLPPELDRVVAQQIRDPYRQRASRSIERF"
     gene            complement(2305..>2724)
                     /locus_tag="VFR00_00225"
     CDS             complement(2305..>2724)
                     /locus_tag="VFR00_00225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0157699.1"
                     /translation="REAILSAVSSGRIEDLRHAYELNELKPDLAAEPVADPVAFWQQI
                     SGDGRGLEVLAALGQILDTGYVVLPTGRDLENNRIYVWPYFAEVPLAGLTPAQDVELM
                     RLLGAATARDFKATGRYSWWRIAIGADGVWHSFRKMP"
BASE COUNT          475 a          927 c          878 g          444 t
ORIGIN      
        1 cttgaggcgg ttgcgcacga tgccggcatc gcgcattagg cgcgccacat ctttggcgcc
       61 gtaacgggcg atgcgctccg cgtcgaaagc atggaaagcg gtgcgaaagt tctcgcgctt
      121 cttgaggatg gtcagccacg acaggccgga ctggaagccc tcgagcacca gcttttcgaa
      181 gagcgcgcga tcctcgctct tgggcacacc ccactcctcg tcgtggtagc gcgcgtattc
      241 cggatcggcg atgccgaccc agggacaacg ccgcagctcg gccgccgtca tagcacctcc
      301 gtgcgcgtcg agggcgtcat ctcgaaccgc gcccaggccg gcttgctgag gcggatgggg
      361 accgcctcgg cgctaaggac gacacccttg gcgagggcct ccgcggcgcg atcgagccgc
      421 accagcgcca gcccacggct gccgtccacc gaaccgatcg ctcccaagct gaccgctccg
      481 gccatgatcg gggcgccggg cgccagcgcg gcagcgccct cgacctgaac cacgcgcttg
      541 cgtccgccgc cgcgatgttg catgcggctc gccacctcct ggccgatgaa gcagccctta
      601 ctgaacgaga cgccgttgag ctgatcgtaa ttggcttcgt gcagaaacgt gtcgcccagc
      661 cgataatccc ggcccgcctc ggggacgccg agcgcgatgc gatggcggtg ccagtcgtct
      721 tgcctcgccg ggcgcgcccc cgtcgcggcc gccagcgccg cttctgccgc aggcggggca
      781 atgatgcgca cgcccaactg cggcaggcgc ggatcgacaa agacggtggc gcccggcaag
      841 gcgacggggc cgatcacccc ctctccccac agcgcaaaga cggccgacgg tacggagact
      901 tcgccgatct cgacgttggc gcgcaacttg tagagcgaaa gccgcttcga caaattcgcc
      961 gcctcggccg cggccgtttc gagcaagaac gccgcctccg cctcgaccac aaagaactcg
     1021 aacaggatct tgccctgagg gctcaacagg ccggcatgca ccgccgcgcc tggaccgatc
     1081 tcatccatgt cgttggtgat gagaccttgc agcagctttt tggcgtcggc accggcgaca
     1141 cgcacgatgc cgcgttcggc gagcagcgcg agattacgag atcgcatgga aaacgtgctg
     1201 tttgtggcgg ccaaagggcc gccgctgcct gatcttcagg caacaggtaa gcgcgtcggc
     1261 tgcaacaggc aagcctccga gcgcggccgg cacggtgccg gcgaccgcca cggccattga
     1321 gggcgccgag aaaaagccgg agcgccgcga cgtttcccgc cgcggccccg gtttgctctt
     1381 acgcccggtg tattctcgcg aacgacgcct tcgctcgacc tagaagcgtt cgatcgagcg
     1441 gctggcgcgc tggcggtagg gatccctgat ttgttgcgcc accacccggt cgagctcggg
     1501 cggcaagcgg tgcacgacgg tgacgggtgt tcccacctcc acacgaccgg ccagatcgac
     1561 gatgtgcgca ttgagcatgc ggaaacaccc cgaggaggcg gcctgcccga tcgatttggc
     1621 gtcgttggtg ccgtggatac ggtagagcga attgccgaga taaagcgcca tggcgccgag
     1681 cgggttcttg atcccgcccg tcatcttttc cggcaggcgc ccatcgcgct cgcgcatttc
     1741 ctccggcgga taccagtcgg gccactccgc tttgcggctg attttctccg ttccggtcca
     1801 ggtgaacccc tcgcggccca cggagatttg atagacgagg gccgtggtcg gcgattgcac
     1861 caggaacagg cggcggctgc gggtgtcgat gacaacactg ccgaccggat agctgctggg
     1921 aaaattgagg ctgggcggcg cctgcgggct gatcgcggga cgcgcgccgc ccgactgctg
     1981 ggccggcccg cgttgtcgcg gccgataaaa gccgtcatcg tcgtcgtccc accagttgaa
     2041 gaggccgtat tgcggttgcg gacggtcata ggaataggcg cgatcggaac gccagctcca
     2101 actctgcgcc tgcgcctgcg gcgcaagtag caggaatcca aaacccaaca gtcccaagcg
     2161 ccacatgatc gtatcctcgt ttggccgatg gcaattgcgg ggggaaaacg tcacggcgac
     2221 ccccgccccc acgggcattt ctgctggcaa cgttatcgtt gctttgaacg gtgcaccagc
     2281 gccttagttc ccggcgccaa gcccttatgg cattttacga aaagaatgcc agacgccatc
     2341 ggcaccgatg gcgatccgcc accaggaata gcgccccgtc gccttgaaat cacgcgcggt
     2401 cgccgcgcca agcagtcgca tcagttccac gtcctgggcg ggggtgaggc cggcgagcgg
     2461 cacctcggca aaatagggcc acacgtagat cctgttgttt tccagatcgc gcccggtcgg
     2521 cagcacgacg tagccggtgt ccaggatctg tccgagcgcc gccaatacct caaggccgcg
     2581 tccgtcgcct gagatttgct gccaaaaggc caccggatcg gccaccggct cggcggcgag
     2641 atcgggcttc agctcgttca actcgtaggc gtgacgcaga tcctcgatgc gcccgctgct
     2701 gaccgcgctc aggatcgcct cgcg
//
LOCUS       DASTMT010000011         2739 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_4267890, whole genome shotgun sequence.
ACCESSION   DASTMT010000011 DASTMT010000000
VERSION     DASTMT010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2739)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2739)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2739
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4267890"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..222)
                     /locus_tag="VFR00_00230"
     CDS             complement(<1..222)
                     /locus_tag="VFR00_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008960292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEU0157700.1"
                     /translation="MVAERKGPLSRFKVIDLTRARAGPTAARHFADWGADVIKVEMPI
                     ESEEDGLGGSRHGPDFFNLHRNKRSLALNL"
     gene            complement(279..1250)
                     /locus_tag="VFR00_00235"
     CDS             complement(279..1250)
                     /locus_tag="VFR00_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011085926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEU0157701.1"
                     /translation="MKAAVLGETGIDIREVAKPVPGPSQVLVKVRAAGLNRADLIMAS
                     GQRHGNAGGPGTVLGLEFAGEVEAVGSEVTSVRPGERVMCSGNGGYAEYAVADWGRVS
                     PIPANNMGYEAAATLPVALQTMHDAVVSNGRLKAGEAVLIQGASSGVGLMGLQIAKLK
                     GARLVIGTSTNAARRARLKEFGADLAVDTNEANWSARVLEATGGAGVDLIVDQVSASV
                     ANENLKATAIRGRIVNVGRLGGFKGEFDFDLHALRRIDYIGVTFRTRTLEEVREIVRR
                     MRADLWGDIEAGRLRLPIDRTFSLDQAAAALAHMRANAHFGKIVLTM"
     gene            complement(1308..2171)
                     /locus_tag="VFR00_00240"
     CDS             complement(1308..2171)
                     /locus_tag="VFR00_00240"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU0157702.1"
                     /translation="MGQAQGTAVRRRGRLARPDAELYWEVTGEGRAIVFAHGLGGNHA
                     SWWQQVAQFATGYACVTFAHRGFSPSSAIVGGPDPGDYADDLAALIDHLGLGEVVIVA
                     QSMGGWTAMEYALQGKAKFGALVLAATTGSIDPARLDAAESWRQRLEEWRQVSEKARA
                     ELRRGDIHIACGARMAREQPGLHLLYRHIDEVNAELDKEALRARLLAARTRAPAELGA
                     IVCPTLLLSGDEDIVMPPFAADALAALLPRAAVRHIADAGHSAYFERAQVFNRHLADF
                     LIACGWAPRRP"
     gene            complement(2230..>2739)
                     /locus_tag="VFR00_00245"
     CDS             complement(2230..>2739)
                     /locus_tag="VFR00_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007756331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEU0157703.1"
                     /translation="DVANTIITHDAGSPRDQLSPFWRPVTPLSYLGWGKTTQLGYGLG
                     LAMGAKLAKPDKLCINVWGDAAIGFTGMDFETAVRERIPILSILLNNFSMAIELKVMP
                     VSTEKYRSTDISGDYAAMARAFGGYGERIEKPSDIVPAIKRGIAATEKGQPALLEFIT
                     SKEVSVSRF"
BASE COUNT          481 a          922 c          879 g          457 t
ORIGIN      
        1 gaggttgagg gcgaggctgc gcttgttgcg atgcagattg aagaaatccg gaccgtgtcg
       61 cgacccgccc aacccatcct cctccgattc gatgggcatc tcgaccttga tgacatcggc
      121 accccaatcg gcgaaatggc gcgccgccgt gggaccggcg cgggcccggg tcaaatcgat
      181 caccttgaaa cgggacagcg gacctttgcg ctctgcgacc attatcgaac aagccctttt
      241 tgccaaatct cgaacgcttg cagcatacgc cggcaagctc acatcgtcag cacgatcttg
      301 ccgaaatgcg cgttggcgcg catgtgcgcc agcgccgcgg cggcctgatc aagcgaaaag
      361 gtacgatcga tcggcaggcg cagtcgaccc gcctcgatgt caccccacag atccgcgcgc
      421 atgcgccgta cgatctcgcg tacctcttcc agggtgcgcg tgcggaaggt gacgccgatg
      481 taatcgatcc ggcgcaaggc gtgcaggtcg aaatcaaatt cacccttgaa cccgccgagc
      541 cgaccgacgt tgacgatgcg cccgcgaatc gcggtcgcct tcaggttttc gttggcaacg
      601 ctggccgaca cctgatcgac aatgagatcg acgccggccc cgcccgtcgc ctcgagcacg
      661 cgcgccgacc aattagcctc gttggtgtcg accgcgaggt cggcaccgaa ctctttgagc
      721 cgcgcgcggc gggcggcgtt ggtcgaggtg ccgatcacga ggcgggctcc cttgagcttg
      781 gcgatctgca gtcccatcag gcccacgccg gagctggcac cctgaatcag cacggcttca
      841 ccggccttga gccgaccatt gctcaccacg gcatcgtgca tggtctgcaa ggcgacgggc
      901 agggtcgcgg ccgcttcgta gcccatgttg ttggccggga tcggcgagac gcggccccag
      961 tccgcgaccg catactccgc atagccaccg ttgcccgagc acattacgcg ctcgccgggc
     1021 cgaaccgacg tcacctcgct gccgacggcc tcgacctcgc cggcgaactc gagcccaagc
     1081 acggtgccgg gtccgccggc gttgccgtga cgctgtcccg acgccatgat caggtcggcc
     1141 cgattgaggc cggcggcacg cactttcacc agcacctgcg agggacccgg caccggcttt
     1201 gctacctcgc ggatgtcgat cccggtttcg ccaagcacgg cagctttcat tggcttgttc
     1261 tccacaatca cgatcccact ttgcgccccg ctcggagccc actcgggtca agggcgccgc
     1321 ggtgcccagc cgcaggcgat gaggaagtcg gcgagatgcc ggttgaagac ctgggcccgt
     1381 tcgaaatagg ccgaatggcc ggcatcggca atatgtctaa ccgcggcgcg cggcagcagc
     1441 gcggcaagcg catcggcggc aaacggaggc atgacaatat cctcgtcccc cgaaagcaac
     1501 agggtcgggc agacgatggc gccgagctcg gcgggggcac gcgtgcgggc ggccagaagc
     1561 cgggcccgca aggcctcctt atcaagctcg gcattgacct catcgatgtg gcgatagagc
     1621 agatgtaggc ccggctgctc gcgggccatg cgggcgccac aggcgatgtg gatgtcgccg
     1681 cgccgcagtt ccgcgcgcgc cttttcgctg acctggcgcc attcctcgag gcgttgacgc
     1741 caggactcgg ccgcatcgag gcgggccgga tcgatacttc cggtggtggc ggcaagcacc
     1801 agggcgccga acttcgcttt gccctgcaag gcgtactcca tggccgtcca gccgcccatc
     1861 gattgggcga cgatgacgac ctcgccaagg cccaaatgat cgatcagggc ggcaaggtcg
     1921 tcggcgtagt cgccgggatc gggcccaccg acaatggccg acgacgggct aaagccgcgg
     1981 tgggcaaagg tcacgcaggc atagcctgtg gcaaactgcg cgacttgctg ccaccaggag
     2041 gcgtgattgc cgcccaggcc gtgtgcaaac acgatggcgc gtccctcgcc ggtgacctcc
     2101 cagtaaagct cggcgtcggg ccgcgccagc cggccgcggc gtcgcaccgc cgtcccctgc
     2161 gcctggccca cgccagagcc gctccctgcc caaggctgcg ttgccaagtc ccggccccgg
     2221 ctcacgtcgt tagaacctgg agacggatac ttccttgctg gtgatgaact cgagcagcgc
     2281 cggttgcccc ttttcggtgg cggcaatacc gcgcttgatg gccggaacga tgtcgctcgg
     2341 cttttcgatc cgctcgccgt agccgccgaa ggctcgcgcc attgccgcgt agtcgccgga
     2401 aatgtcggtt gaccgatatt tctcggtgga aaccggcatc accttgagct cgatcgccat
     2461 cgagaagttg ttgagcagaa tggaaaggat gggaatgcgc tcgcgcactg ccgtctcgaa
     2521 atccatgccc gtgaagccga tggccgcatc gccccataca ttgatgcaga gcttgtccgg
     2581 tttggcgagc ttggcaccca tggcaagccc caaaccgtag ccgagctgcg tggtcttgcc
     2641 ccagcccaga tacgacagcg gcgtcaccgg ccgccagaat ggggagagct ggtcgcgcgg
     2701 gctgccggcg tcgtgagtta tgatggtatt ggcgacgtc
//
LOCUS       DASTMT010000012         3128 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_1318320, whole genome shotgun sequence.
ACCESSION   DASTMT010000012 DASTMT010000000
VERSION     DASTMT010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3128
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1318320"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..310
                     /locus_tag="VFR00_00250"
     CDS             <1..310
                     /locus_tag="VFR00_00250"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEU0157704.1"
                     /translation="REVKARHGLRPDQSSRDILPPDADPFHEIFERIPPVVARWLAAH
                     PNELLLFVGHSGVFDALHQHLIGPRSGRESDHAVPYLADPIPTGWELRALRKPSLTRS
                     "
     gene            713..997
                     /locus_tag="VFR00_00255"
     CDS             713..997
                     /locus_tag="VFR00_00255"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HEU0157705.1"
                     /translation="MIIVAGRLYVRPERREAFLAASAVAVNAARRSAGCLDFVVAADP
                     IEPDRVNVYERWDTEASLLAFRGEGPSSELTSEILRADVQRHYVSSSGPP"
     gene            complement(1110..1790)
                     /locus_tag="VFR00_00260"
     CDS             complement(1110..1790)
                     /locus_tag="VFR00_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007068552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HEU0157706.1"
                     /translation="MRLVAGLLILDLLIAPARAQTPPSAAEIAAYQGLHLVAARGDVT
                     AAQQALAAGAKPNATDAQQRSPLHVAAHFGHGEVARILLAAGADPRALDAQRYDILTI
                     AAVRNDLAFVQLALQLGADARAITSPYQGTALIAAAHLGHGPVLEALIGAEAPLDHVN
                     NLGWTALIEAIVLGDGGLNHTQCVRILLEAGANPNLPDGAGRSPLALARARGYGEMAG
                     LIAAAGGR"
     gene            2291..>3128
                     /locus_tag="VFR00_00265"
     CDS             2291..>3128
                     /locus_tag="VFR00_00265"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HEU0157707.1"
                     /translation="MTISKGSATMQLNAMFPTRDIGNDPAKIRDWAQAAEDLGFAYIE
                     VPDHVFGAAARDGWTPLYNETDPFHETFVLLGFLAAVTKRIRLSSGVLIAPQRQTGLI
                     AKQAAEADLLSAGRLRLGIGVGWNHVEYQALGEDWKTRGARQAEQVEVLRRLWSEDLV
                     SFKGRFHALFDVNIQPPPVQRPIPIWFGGVSDAVVMRAARLGDGWMPIMAPDEKAEQK
                     LALLRGELKQHGRALNSFGIEGWLRMHEADPQRWAAAADGWRKLGADMVMLYPMYRMS
                     RFD"
BASE COUNT          488 a          981 c         1062 g          597 t
ORIGIN      
        1 tcgcgaggtc aaagcgcggc acggcttgcg gcccgatcaa tcctcccgcg acatcctgcc
       61 cccggacgcg gatccctttc acgagatctt cgagcgcatc ccgcccgttg ttgccaggtg
      121 gctggcggcg catcccaacg agctgttgtt attcgtcggc cacagcggtg tgttcgatgc
      181 cctgcaccag catctcatcg gcccacgctc gggccgtgag tcggaccacg ctgttcccta
      241 tcttgccgat ccgatcccca ccggatggga gttgcgggcg ctgcgcaagc cgtcgctcac
      301 gcggagttga gcagtatttc ggcggacgaa gctgtcatcg cctccctcca tggctgtgcc
      361 ggatcgcttt tcccaattga gcctgcgatc acggcgggcc aacgcccgca gtgcgtgtgc
      421 ggcaatgggc caccgcgcaa accggcggtg cccgcgcccc ccgcgcttct cggcgcccga
      481 gccaacgctt gcccgccagg caggtctggc gccaactgcc agcaggtcgc gccaggagca
      541 acgccattcc cgcgcgactg tttcagtctt ttgtttgcaa ggcaaacgct tgcggccggc
      601 ctgcccccgc gtgctgcggt gggtgccgca acaattccga cagcttttgg cgctatggcg
      661 aggcgtcggc tgccctcggc tttccattgt taatcccagg cgagttcttg ccatgatcat
      721 cgtagcggga cggctctacg tgcgaccaga gcggcgcgag gccttcctgg ccgcctccgc
      781 agtggcggtc aatgcggccc gccgcagcgc cggctgcctc gactttgtgg tggccgccga
      841 tcccatcgaa cccgatcggg tcaacgtcta tgagcggtgg gacacggagg cgtcactgtt
      901 ggcctttcgc ggcgaagggc cgagctccga gctcacctcg gagatactgc gcgcggacgt
      961 gcaaaggcat tatgtgtcct cgtcggggcc accctagccg tcgctgcgat cccgttgggc
     1021 aatggcgttc cccaccgtgt ggtgatccga aaagcgggct ttttttgcgc ggccgatgtt
     1081 tgcggggcct gggtacgggc tcggcgatgc tatcgtccac cggcggccgc aatcagtcct
     1141 gccatttccc cgtatccgcg cgctcgcgcg agcgcaagcg gtgagcggcc agcgccgtcg
     1201 ggcaggtttg gattggcacc agcctcgagc aggattctga cgcattgggt atggttgagg
     1261 ccaccgtcac cgagaacgat cgcttcgatc agagcggtcc agccgagatt gttgacatgg
     1321 tctagtggtg cctcagcgcc gatcagcgcc tccagcacgg ggccgtgtcc aagatgcgcc
     1381 gcggcaatga gcgctgtccc ctggtacggg ctcgtgatcg cccttgcgtc ggcgcctaat
     1441 tgcagggcca gttgcacaaa ggccagatca ttgcgcacgg cggcgatggt cagaatgtcg
     1501 tagcgctggg cgtcgagagc gcgcggatcg gcacccgcgg ccagcaaaat gcgggcgacc
     1561 tcgccatggc cgaagtgcgc ggcaacgtga agtgggctgc gttgctgggc gtcggtggcg
     1621 ttgggtttgg cgcccgccgc aagcgcttgc tgcgccgcgg tgacgtcgcc cctggccgct
     1681 acgaggtgca aaccttggta agcggcaatc tccgcggcac ttggcggcgt ttgcgcccgc
     1741 gcgggggcaa tgaggaggtc caggatcagc aggccggcga ccagacgcat cacgccgctt
     1801 cctccgcttc tacttggggt tggcgagtgt agcctggttt ttgtggcggc gggagtgcga
     1861 tggagacatc cgaattgacc atcgccaacc tcgaattttg gtgatcgcgg gggtgcttct
     1921 tgtcgcatgg ccccaggcgg ccctgatcga aggcgccgcc ggcatcgatc tttttgatca
     1981 gtttgaggtt gttgccgtcg tcagcgccgc ggagcctatc gttaacccca cgccaatagc
     2041 tcaccggtta acgcccgcgc tgtggatatc ggcggcgatg taccggccat tggcgcgacg
     2101 caaccgacga gggggcgttt ggcgcaggtg tggccgggcc gctatgacgg cttttgagtc
     2161 gcatttggcc agcgggcggt ggccgattgc tgatcggcgc gcgcgacacg gccagtacgc
     2221 tagccagtac gctaggcggt acgctactag gcagtgcgcg aggcagtgcg ctgggctatg
     2281 agttgatggc gtgacgatca gcaaagggag cgcgaccatg cagctcaatg ccatgttccc
     2341 gacgcgtgat atcggcaacg atccggcgaa aatacgcgat tgggcgcagg ccgccgagga
     2401 cttgggcttt gcctatatcg aagtccccga tcatgtgttc ggcgcggcgg cccgtgatgg
     2461 ttggacgccc ctctacaacg aaacggaccc ttttcacgag accttcgtgc tgctcggctt
     2521 tctcgcggcc gtgaccaagc gcattcggct atcgagcggg gtgctgatcg cgccgcagcg
     2581 tcaaaccggc ctgatcgcca aacaggccgc ggaggccgat ctcctgagcg ccggccggct
     2641 gaggcttggc atcggcgtgg gctggaacca tgtggagtac caggccctgg gggaagattg
     2701 gaagacgcgc ggcgcccgcc aggccgagca ggtggaggtg ttgcggcggc tatggtctga
     2761 agatctggtc tcgttcaagg gccgctttca tgcgcttttc gacgtcaaca tccaaccccc
     2821 gccggtgcag cgtccgattc ccatctggtt tggcggcgtt tccgatgctg tggtgatgcg
     2881 ggcggcacga ctaggcgacg gctggatgcc gatcatggcg cccgatgaaa aggccgagca
     2941 gaagctggcg ctgttgcgcg gagagctgaa gcaacacggg cgcgcgttaa actccttcgg
     3001 gattgagggc tggctgcgca tgcacgaggc ggacccgcag cgctgggcgg ccgcggccga
     3061 cggctggcgt aaactcggcg ccgacatggt gatgctttat cccatgtatc gcatgtcgcg
     3121 tttcgacg
//
LOCUS       DASTMT010000013         2364 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_2630290, whole genome shotgun sequence.
ACCESSION   DASTMT010000013 DASTMT010000000
VERSION     DASTMT010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2364)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2364)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2364
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2630290"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..318
                     /locus_tag="VFR00_00270"
     CDS             <1..318
                     /locus_tag="VFR00_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HEU0157708.1"
                     /translation="AIYFRHIGTAADANADSKLSDRLKDLVSKKQYRRARIIGAAIRA
                     AHMLSIGRPGIVDETTLSYERDRLVLTLPRAYAALDGERLRRRFAVLASLLERVPEVR
                     LAA"
     gene            448..1371
                     /locus_tag="VFR00_00275"
     CDS             448..1371
                     /locus_tag="VFR00_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HEU0157709.1"
                     /translation="MAEDPYKVLGVPRTATDEEIRRTYRKLAKELHPDLNPSNQAVAE
                     ERFKKVSAAYDIVGDPEKRKQYDRGEIDANGEQRRGYQRAHAGGNPFGGRTGTGRAGD
                     EFGFGDIFSDLFGSMRAGRGADAGAPFRMRGQDIRYTLEVDFLEAVNGTTKRVTMPEG
                     GVLDLNVPEGVADGQILRLRGKGGAAGRGGEPGDALIEVRVRPHQQFKRSGDDITLDL
                     PIALDEAVIGAKVEVPTISGRVQLTIPKGTSSGRVFRLKGKGVRNGASGNTGDQLVTV
                     HIVLPDKIDDELSYFLSEWRLKHRYNPGRSS"
     gene            1528..1680
                     /locus_tag="VFR00_00280"
     CDS             1528..1680
                     /locus_tag="VFR00_00280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0157710.1"
                     /translation="MKMIIIGGVLTAALLFAVVSSIKADGPHWVAGGLIADGPAFGSA
                     GRANGA"
     gene            1783..>2364
                     /locus_tag="VFR00_00285"
     CDS             1783..>2364
                     /locus_tag="VFR00_00285"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEU0157711.1"
                     /translation="MHFGQFNLMGYRTPGAKAHELYDNAIEQVKAAEANGFEIAWFAE
                     HHFSNYCVCPSPLMMLARLAGETARIRLGSAVVVTPLYQPVRLISEIGMVDALSHGRL
                     VLGVGSGYQPYEFERFGEQLEHSVPKLFEFMEMLELAFNRDTFSYQGRYFRLPETHIA
                     SRPLGPPPEIWVAGDNAQLHQMAARKGWTVMLTP"
BASE COUNT          434 a          769 c          759 g          402 t
ORIGIN      
        1 gccatctact tccgccatat cggcacggcc gccgatgcca acgccgacag caaattgtcg
       61 gaccggctca aggacctggt cagcaaaaag caataccggc gtgcccggat catcggagcc
      121 gcgattcgcg ccgcccacat gctttccatc ggccgccccg gtattgtcga tgaaaccacc
      181 ctgtcctacg agcgagatcg cttggttctg accctgccaa gggcctatgc ggcgctcgat
      241 ggcgagcgcc tgcgccgacg ttttgccgtg ttggcaagtt tgttggaacg cgtgcccgag
      301 gtgcgcctgg cggcataagg gggtaccgca gggcctccgg cgggccgccc cccgtgttga
      361 tggcgccgag agcgcctata gattggcgca cggacgccac gatcctatct tagggtcgac
      421 agtgagctgg actggatgga cgttgcaatg gctgaagatc cttacaaggt gctaggcgtg
      481 ccgcgcacgg caaccgacga agagatccgc cgcacctacc ggaaactggc caaggagctg
      541 catccggacc tcaaccccag caaccaagcc gtggccgagg agcggtttaa gaaggtgtcg
      601 gcggcctacg acatcgtagg cgaccccgaa aagcgcaagc agtatgatcg cggcgagatt
      661 gacgccaacg gcgagcagcg ccgcggctat caacgcgccc atgccggcgg caacccgttc
      721 ggcggccgca cggggactgg ccgcgccggt gacgagttcg gcttcggcga cattttctcc
      781 gatctgtttg gctccatgcg ggcagggcgc ggcgccgacg ccggcgcgcc cttccgcatg
      841 cgggggcagg acattcgcta cacgctggag gtcgattttc tggaggccgt caacgggacc
      901 accaagcgcg taaccatgcc cgaaggcggg gtgctcgatc tcaacgtacc cgaaggcgtc
      961 gctgacgggc agattctgcg cctgcgcggc aagggcggcg ccgccggccg cggcggcgag
     1021 cccggcgatg cgctgatcga ggtgcgcgtg cgcccgcatc aacagttcaa gcgctcaggc
     1081 gacgacatca ccctcgattt gccgatcgcc ctcgatgagg ccgtgatcgg cgccaaggtc
     1141 gaggtcccaa ccatctctgg acgtgtgcag ctgaccatcc ccaagggtac cagctcgggg
     1201 cgcgtgttcc ggctcaaagg caagggcgtc cgcaatggcg ccagcggcaa cacgggcgac
     1261 cagttggtaa cggtgcacat cgtgctcccc gacaagatcg acgatgagct ctcctacttt
     1321 ctgtccgagt ggcgcttgaa gcaccgctac aacccgggac gctcgagctg agctcgaccc
     1381 gcgctcctaa agccggttca aacctcgtca cggccctgtg acacggcctt ttctggccgt
     1441 ccgttgacgc catatcaggc gcttgaaaga gacaaccgcc gcgcctcgca caaggctcga
     1501 ggcaaccgat ccgctaagga acagaaaatg aaaatgatta tcattggcgg ggtcctgacg
     1561 gcagcgctgc tcttcgccgt ggtatcctcg atcaaggccg atggaccgca ctgggtcgcc
     1621 ggtggcctca tcgccgacgg ccccgcgttc gggagcgcgg gccgggcaaa tggcgcctga
     1681 gcgccgccgc gctggctgcc agattgccgt tgctgagccc tgccccccat gccttactgt
     1741 gcgccgccgc acgacggggc atgacctgcg aggagggcgt tgatgcactt cggccaattc
     1801 aacctgatgg gctaccgtac gcccggcgcc aaggcgcatg agctttacga taacgccatc
     1861 gagcaggtga aggcggccga ggccaacggc ttcgaaattg cctggttcgc cgagcatcac
     1921 ttttccaact attgcgtgtg tccctcgccc ttgatgatgc tggcgcgact ggcgggcgaa
     1981 acggcgcgca tcaggttggg ctcggcggtg gtggtgacgc cgctctatca gcccgtgcgg
     2041 ctgatttcgg aaatcggcat ggtcgatgcc ctgagccacg gccggctcgt gctgggggtg
     2101 ggaagtggct accagcccta cgagttcgag cgctttggcg agcagttgga acactccgtg
     2161 cccaagctgt tcgaattcat ggaaatgctg gaactcgcct tcaaccgcga caccttcagc
     2221 taccagggca gatacttccg cttacccgaa acccacattg cctcccgccc gctcggtccg
     2281 ccgccggaaa tttgggtcgc cggcgacaat gcgcagttgc accagatggc ggcgcgcaaa
     2341 ggctggacgg tgatgctgac gcca
//
LOCUS       DASTMT010000014         4647 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_5581715, whole genome shotgun sequence.
ACCESSION   DASTMT010000014 DASTMT010000000
VERSION     DASTMT010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4647)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4647)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4647
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5581715"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1111
                     /locus_tag="VFR00_00290"
     CDS             <1..1111
                     /locus_tag="VFR00_00290"
                     /inference="COORDINATES: protein motif:HMM:NF023649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3604 domain-containing protein"
                     /protein_id="HEU0157712.1"
                     /translation="TSDAHDAHALFAKLKGRDCVVMAHVGGRYADIKFAHDPMETAVE
                     VHSAWGTFEWLLRDAFAANYRVGIVANSDGHKGRPGACYPGASFFGSTGGLTCFLAER
                     LDRAAIFEAMRRRHHYATTGNRAFLSVTVETPGDALVFTRDPAAGPTTSQPTRRLLMG
                     DIARLGESEVELCVEVIGSAPIERLDLWDGLDHLETIRPYAAAELGARVRLLYEGAEY
                     RGRARTTIWDGELSLEGNRIVRAEIINNWNLERGIERQSVDRVRWKAVTTGNYGGIDL
                     WLAEAHAGRLCFTTTPVAGAIAIAEIGIDERVFPAGGLERAIKLHRLPTQLSRRHLAL
                     KRRLPLRGKGDTRLFVRVQQEDGHRIWSSPIYLSR"
     gene            complement(1175..1591)
                     /locus_tag="VFR00_00295"
     CDS             complement(1175..1591)
                     /locus_tag="VFR00_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013165376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF126 domain-containing protein"
                     /protein_id="HEU0157713.1"
                     /translation="MSIVLKCHPGIGPTVSGTALVAQDNFSARYDLDRHNGTFSRPTH
                     KLFGQSYHGKILVLVTAKGGVASAWMLRDMQARGIVPKAILFDRSNTILAQGAAFAEL
                     SLLDRFEAGDPTTLIRNDDELLVEPAAGRVTILNRS"
     gene            complement(1591..2838)
                     /locus_tag="VFR00_00300"
     CDS             complement(1591..2838)
                     /locus_tag="VFR00_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013165377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase X catalytic domain-containing protein"
                     /protein_id="HEU0157714.1"
                     /translation="MRLNEEEQAMLAGELGRARQWALRHQLAVGDFFDAPDFVRVGQA
                     HVMADTESLGPSGVAWLEELAALPATERRVMVPTITDPRGLDFASYKRLRQTEAMADL
                     EARTIAAFEALGVLMTNTCINYQTILPPVRGEHMAFGDTGVVIYCNSVMGARSNFEGG
                     PSALAAGLSGRTPRYGYHLDAKRAGSKHFAVRAQPRDLADWGALGGLVGRICNSYWEV
                     PVLTGLAAPPSSDELKHFGAALASFGSVALFHIPGLTPEATDLAAAFAGAPVPAAISI
                     EAQDIAAFYAAYAAPGEKVDVVVFAAPQLSLLELGQVAELLAGRCISAETTLIVATSP
                     ELKFAADRMGLTAAIEGAGGIVAAGVCFYQSYAREMAEANGWQRLLTNSAKLVNIIGG
                     YGYQPTLASMERCIDSAVAGRIV"
     gene            complement(2879..3850)
                     /locus_tag="VFR00_00305"
     CDS             complement(2879..3850)
                     /locus_tag="VFR00_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018319710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEU0157715.1"
                     /translation="MLRGLSVVALALALLAAPAEAAEANYPSRSISLIVPFAAGGPTD
                     VVARIVGDHMSRTLGQQLVVENIGGAGGTTGMAKVAAAEPDGYTLGIGNMGTQSAAPA
                     LYPNLKYDPAASFAQVGIANFTPQAIVAKKDNPAKDLKEFIAYLKANQAKLSYGHAGV
                     GSISHVSGTLFNAQFGLKPALVAYRGTAPALNDLVGGQIDYMVDQSLNVIPQIKAGTI
                     RVFAVAAAQRLESLPDVPTTKEAGVDFIFSAWNAMVAPKGTPKEIIDKLTAALNKALD
                     DPATVKRYVDLGSTAPEAGERGPDGLQRLVESEMARVTPVLKEAIAK"
     gene            4074..>4647
                     /locus_tag="VFR00_00310"
     CDS             4074..>4647
                     /locus_tag="VFR00_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019404818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0157716.1"
                     /translation="MRFRPLAVVSALVLALAATPAAAKTFRYAFQGDLNALDPYTLNE
                     SFTLGALGNVMEGLTKRDKDLKIIPGLAERWEIVDPLKWRFYLRKGVKFHGGEDFTAD
                     DVIFSLERMRSPTSQISTRAPADMQAVKVDDYTVDFVLTSPNPILHAEWDTWYMFSKK
                     WSEANGATQAQSAKATSLNPFALKANGTGPF"
BASE COUNT          770 a         1598 c         1489 g          790 t
ORIGIN      
        1 gacctccgac gcccacgatg cgcacgcgct gttcgccaag ctgaagggca gggattgcgt
       61 ggtgatggcc catgtcggtg gccgctacgc cgacatcaag ttcgcccacg acccgatgga
      121 gacggccgtc gaggtgcact cggcctgggg cacctttgaa tggctgctgc gcgatgcctt
      181 cgctgcgaac taccgggtcg gcatcgttgc caattccgat ggtcacaagg gtaggcccgg
      241 cgcctgctat cccggcgcct cgttcttcgg cagtaccggc ggcctcacct gctttttggc
      301 cgagcgcctc gaccgcgccg ccatcttcga ggcgatgcgg cggcggcacc actacgccac
      361 cacaggcaat cgcgccttcc tgagcgtcac cgtcgagacc ccgggggatg cgctggtctt
      421 tacccgcgat cctgctgccg gaccgaccac ttcgcagcca acgcgccgcc tgctcatggg
      481 cgatattgcc cgccttgggg agagcgaggt tgagctctgc gtcgaggtga tcggctcggc
      541 gccgatcgag cgcctcgatc tgtgggacgg gctcgaccac ctggaaacca tccgccccta
      601 cgcggccgcc gaactcggcg cccgcgtgcg cctcctctat gagggggccg aatatcgcgg
      661 ccgggcccgc accacgatct gggacggcga gctcagcctc gagggcaacc gcatcgtgcg
      721 tgccgagatc attaacaatt ggaacctcga gcgcggcatc gagcggcaaa gcgtcgaccg
      781 cgtccgctgg aaggccgtga ccaccggcaa ctatggcggc atcgatttgt ggctcgccga
      841 ggcgcacgcc gggcgccttt gcttcaccac cacccccgtc gcgggcgcga tcgccattgc
      901 cgagattggc attgacgagc gcgtgttccc ggccggtgga ctcgagcggg ccatcaagct
      961 gcacaggctg ccgacccagc tgagccgtcg ccacctcgcg ctcaaacgca ggctacccct
     1021 gcgcgggaaa ggcgacacgc gcctgttcgt gcgcgtgcag caggaggacg gccaccgcat
     1081 ctggtcgagc cccatctatc tcagccgctg actcagccgc tgagacaaga cgtgccgccg
     1141 gcgccagccg ccaagctgtc ccgcggctcg acgctcagga gcgattgagg atcgtcaccc
     1201 gcccggccgc gggctcgacc aggagttcgt catcgttccg gatcagagtg gttggatccc
     1261 ccgcctcgaa ccggtcgagc aagctcagct cggcgaaggc ggcgccctgg gccaaaatag
     1321 tattgctgcg atcgaacaga atggccttcg gcacgatgcc gcgtgcctgc atgtcacgca
     1381 gcatccaggc cgaggcgacc ccgcccttgg ccgtgaccag cacgaggatc ttgccgtggt
     1441 agctctggcc aaaaagtttg tgcgtgggac gcgagaaggt gccgttgtgc cgatccagat
     1501 cgtagcgcgc ggagaaatta tcctgcgcca ccagcgccgt cccgctcacc gtcgggccaa
     1561 ttcccggatg gcatttcaag acgatggaca tcacacgatc cgcccggcaa ccgcgctatc
     1621 gatgcaacgc tccatgctgg ccagcgtcgg ctgatagccg tagccgccga tgatgttgac
     1681 gagcttggcc gagttggtga gcaggcgctg ccagccgttc gcctcggcca tttcgcgggc
     1741 gtagctttgg tagaagcaga ccccggccgc gacgatgccg ccggcccctt cgatcgcggc
     1801 ggtgagcccc atgcgatcgg cggcgaattt gagttccggc gaggtggcaa cgatcagcgt
     1861 cgtctcggcg ctgatgcagc ggcccgccag aagctcggcg acctgtccga gctccagcag
     1921 cgacagctgg ggtgcggcga acacgaccac gtcgaccttc tcgccgggtg ccgcataggc
     1981 cgcgtagaag gccgcgatat cctgcgcctc gatgctgatc gcggcgggca cgggagcccc
     2041 ggcaaaggcc gccgccaagt ccgtcgcctc cggcgtcagg ccgggaatgt ggaagagggc
     2101 aaccgagccg aagctcgcca gcgccgcgcc gaaatgcttc agttcgtccg agctcggtgg
     2161 cgcggccaag cccgtcagga cgggcacctc ccaatagctg ttgcagatgc gcccgacgag
     2221 gccgccgagc gccccccagt cggcaaggtc gcgcggctgg gcgcgcacgg cgaaatgctt
     2281 gctgccggcg cgcttggcgt cgaggtgata gccgtagcgc ggggtgcgtc cgctgagacc
     2341 tgccgccagc gccgaaggcc caccttcgaa attgctgcgc gcccccatca cgctgttgca
     2401 atagatgacg acaccggtat cgccgaaggc catgtgctcg ccgcgcaccg gcggcagaat
     2461 ggtttgatag ttgatgcagg tgttggtcat aagcacgccg agcgcctcga aagcggcgat
     2521 ggtacgcgcc tcgagatcgg ccatggcctc ggtctgtctc aggcgcttat agctggcaaa
     2581 atcgaggccg cgcggatcgg tgatggtggg caccatgacg cgccgttccg ttgcgggcag
     2641 cgcggccagc tcttccagcc aggccacccc cgagggcccg agactttccg tgtcggccat
     2701 gacgtgggcc tggcccacgc gcacgaaatc cggcgcatcg aagaaatcgc caacggccag
     2761 ttgatgccgc agcgcccatt ggcgggcgcg gcccaactct ccggccagca tcgcttgctc
     2821 ttcctcgttg agccgcatct cgtcctcgtg ctcgcgcctg ccgcgggcgc cagggccgct
     2881 atttcgcgat ggcctccttg agcacgggag tgacgcgcgc catctcgctc tcgaccagcc
     2941 tttgcaggcc gtcggggccc cgctcgccag cctccggcgc ggtgctgccg aggtcgacat
     3001 agcgcttgac ggtggctgga tcgtcgagcg ccttattcag cgccgccgtc aatttgtcga
     3061 tgatctcctt gggcgtgccc ttgggcgcca ccatcgcatt ccaagcacta aagatgaaat
     3121 cgacgccggc ttccttggtg gtgggcacgt ccggcaggct ctcgaggcgc tgcgcggcgg
     3181 caacggcgaa taccctgatg gtgcccgcct tgatctgggg gatcacgttc aaggactggt
     3241 cgaccatgta gtcgatctgg ccccccacca ggtcgttgag ggccggcgcg gtcccccggt
     3301 aggccaccag cgcgggcttg aggccgaact gggcattgaa cagggtaccg gagacgtggg
     3361 agatcgagcc gaccccggcg tggccgtagc tgagtttggc ctggttcgcc ttgagatagg
     3421 cgatgaactc cttcaggtcc ttggccggat tatccttctt ggccacgatc gcctgcggcg
     3481 tgaaattggc gatgccgacc tgcgcaaaac tcgcggccgg atcgtacttg agattggggt
     3541 aaagggccgg cgccgccgat tgcgtgccca tattgccgat gcccagcgtg taaccgtcgg
     3601 gctcggcggc ggcgactttc gccatacccg tggtgccacc cgcgccgccg atgttctcga
     3661 ccacgagttg ctggccgagc gtacgcgaca tgtgatcgcc gacgatgcgt gccaccacat
     3721 cggtcggccc acccgcggcg aacggcacga tcaggctgat cgagcggctc gggtaatttg
     3781 cctcggccgc ctcggcaggc gccgcgagca gggccagcgc cagcgcgaca acacttagac
     3841 ctcgaagcat tttacctctc ctcttgccgg cgcctccgcg gccatggccg ccccggtctt
     3901 ccggcccagg caatgggcgc attccccgca atctcaacca tcccacccgg ctttggcaac
     3961 cgcggcgctc ggcctcggcg ctgtgccccc ggcatggctg gaccaacgct acaagttcgg
     4021 ccaatatccc gctggcttat ctgctcccca cgtccttgct gtgagagacc tacatgagat
     4081 ttcgacccct tgccgtcgtg agcgcgctcg tgctcgcgct ggcggccacg ccggctgccg
     4141 caaaaacatt tcgctatgcc ttccaaggcg atctcaacgc gcttgatcct tataccctca
     4201 acgagagctt cacgctcggc gccctcggca acgtcatgga gggcctgacc aagcgcgaca
     4261 aggacctgaa aatcatcccg ggcctggccg agcgctggga gatcgtcgac cccctgaagt
     4321 ggcgctttta tctgcgcaag ggcgtcaagt ttcacggcgg ggaggacttc accgccgatg
     4381 acgtgatctt ctcgctggaa cgcatgcgca gtcccacctc gcagatcagt acacgcgccc
     4441 cggccgatat gcaggccgtc aaggtcgacg actacacggt cgacttcgtg ctgacctcgc
     4501 ccaatcccat cctgcatgcg gaatgggaca cctggtacat gttttccaag aagtggtcgg
     4561 aggccaacgg cgccacgcag gcgcagtcgg cgaaggcgac ctcgctcaat ccgttcgcgc
     4621 tcaaggccaa cggcaccggc cccttca
//
LOCUS       DASTMT010000015        11063 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_2959013, whole genome shotgun sequence.
ACCESSION   DASTMT010000015 DASTMT010000000
VERSION     DASTMT010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 11063)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11063)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11063
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2959013"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(117..1256)
                     /locus_tag="VFR00_00315"
     CDS             complement(117..1256)
                     /locus_tag="VFR00_00315"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0157717.1"
                     /translation="MSVLFGLGAAAAQEPIVFGVITPLSPPGETALGQLAKRGAEIGA
                     EYINEKGGVLGRKVALSVQDSAGKNEQAVAAYRRLVSNDKAVAVFGFIHSGANIAVNE
                     VAKEMGVPTMGTQTGASDVTAKHYDVAFRTHAVDPPRAATWLGWIKKNNYKRVSILAE
                     TTDYGIGLAKETEKQNKDKALGLEIQTIMFDRTTTDLLPQLLQVKAFKPDAIVNVGVG
                     QPLDLMITQAHTVGLLPGAAMVTSYDAPARPQFWQLHGDKGVGINFVAFYSPKSELSE
                     VGKEFAKRYQAKFNEPPVYAALNGFANALVFAEAIEKAKSTEPKAIIKALEEGSFSGW
                     SKTPITFPRAEGALWHNWSPPLMVLNYAAKDQTPATATVAYVLGE"
     gene            complement(1417..2508)
                     /locus_tag="VFR00_00320"
     CDS             complement(1417..2508)
                     /locus_tag="VFR00_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0157718.1"
                     /translation="MARACDILIFGSGSFAQRIACDLASASKEPVSVAIAGRNPARLA
                     WIATAARARAHMFGCPLELATVEVDLAAGGAAGTVLAKLEPAVIVQAASVQAGAVIAA
                     GGNAWTRLVAEGGLSATAVFQALLTSRIARAAAELGLKSQLINCCFPDVANAIVMGMG
                     LPVLCGIGNIAILAHAFAGSLAAARVAPDLKLLCHYQNIGAWRRPPAERAGPAARVWL
                     DGREIGDVFDRFAEVQLTPEPVIDISGASGVTLMLALAAGRDWRGHVPGPVGLPGGYP
                     VAISNGRLALDLPPGLSPTDAVAWNARYEAENGLSVAADGRVHYAGRLRDRLEAESPA
                     LAAGFHVDDLEPVTLEFARLRDRLLARPL"
     gene            2600..3820
                     /locus_tag="VFR00_00325"
     CDS             2600..3820
                     /locus_tag="VFR00_00325"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU0157719.1"
                     /translation="MVSASADVAIVGGGLVGCFAAYFLRARGRSVIILEKGAAAAAAS
                     GSNFGNLRLQGRHPAQFPLSLRAQAIWEDLARLSGEACGLVACGHLYLGLAASDQPKL
                     ATAAGAALAAGLDVELLTGAAARHRWPMLSSLVTGASWSKRDAVAEPGRVAPIVARLA
                     VRAGAELIEHTKVVRIERAAGGFAIGTDGGANIRSDYLINAAGAWGGEIAAAYGEPVP
                     LFAAGPPLFALRPEHPWEGPSLHAIDGTVLLRQGEGGAAVAGAFPRVAADLASGVARV
                     PSDRLARGLERLGAVLPGLGRLLPGRVWSGVEGYLPDMLPIIGWSGSTPGLVHAFGFS
                     GHGFQLAPGVGAVLAELIATGVTETPIEAFSLARFGGEVAFDDKLWHEFDAELVARFR
                     KQAAPDVEAGGRSP"
     gene            3896..4711
                     /locus_tag="VFR00_00330"
     CDS             3896..4711
                     /locus_tag="VFR00_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006469648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEU0157720.1"
                     /translation="MSNERSVFFDISDGLAMLTLNKPAKLNAWDTPMRAEIAGLLREW
                     NVDQRVGAVIVTGAGDRAFSAGQDLDETEKFQSGSDGANWFQSWRDFYNSIRNLDKPC
                     LAALNGVAAGSAFQAAMLMDVRIGHSGVRMGQAEINSGIPSVTGPMLMLPRIGLSRTV
                     ELTLTGRMMDAAECHAVGLIHYLVERPEDVLEKTREVARLLAAKPPIAMRLTKTRFRQ
                     VTQPAFEEAFENGSVYQAEAFASGEPQAAMRVFFAERAARRKARAAGKASDAT"
     gene            4701..6260
                     /locus_tag="VFR00_00335"
     CDS             4701..6260
                     /locus_tag="VFR00_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEU0157721.1"
                     /translation="MRPEAAVAQLRAPFPDTDAGFVGLFDSQARATPQRSFAILGGEA
                     LSFAALDRTSRALGAWLRAKGLGPGDRVALMLRNGETALSLMLALARTGSIWVPINTQ
                     AVGDGLAYVLGHAEPSLIIADSDQLPALSACGVAAAARAIAVEDAAREAARAPASEAL
                     MVVPPADAPFAIMYTSGTTGRPKGVLVSHRMLRLSGEAAALVADPSPGDVLYLWEPLF
                     HIGGAQMLVLPLIRDVTLAIAERFSARGFWTDVQGKGASHIHFLGGILQILLKQPPGP
                     LDRGHGVRIAWGGGCPVDVWQAFQDRFGVEIRECYGMTECSSVTTANLDAPIGSVGKP
                     VPWFDVAVLDAAGRPVAPGVRGEMVVTERLGGALTRGYFKDGDATARAFRAQAFLTGD
                     LVSYDSDGNYYFHGRMTDSVRVRGENVTALEVESVARTHPAVEDCAMIGVAAEVGEAE
                     IKLFVTLRQGVELEPQELSAWLADRLPRYQRPRYITVLDAFERTPSARIMKHALSRRT
                     DDCWDLAAVGI"
     gene            6334..7599
                     /locus_tag="VFR00_00340"
     CDS             6334..7599
                     /locus_tag="VFR00_00340"
                     /inference="COORDINATES: protein motif:HMM:NF018682.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HEU0157722.1"
                     /translation="MTKILILADDLSGAADCAGGCAAAGLDTLVLLEAAGAGPAMASV
                     LAIDLNSRDKSPAAAGEAIARTIGQMRDLGAKILYHKIDSTLRGNWAHELVAARASLA
                     VAHGASPLALVVPAFPARGRITRSGRVLVTASRLLGAEASAPAPMVAAGEVAAPLRER
                     GLSVRILARPEVEAPAWRAAGLFDAIARSGVDAVVGDAESDCDLAAITAAALAAHVPL
                     LWVGSAGLMRALAQILSRKAPAPPARPPTARGPLLFVVGSTSAVSRTQFERLIAEPGV
                     AGFCVSPEEVRGAPRRLEAYLDGARARGCDAALRLVGDSPLATPHDPSLVSALADIVG
                     PRLPGFGGLVATGGATARGLIEKAGYSAIALGGEVEAGVPWGLALRDAASGGIGLEGR
                     GLPLVTKAGGFGDADTLLVCRKALKVSET"
     gene            7596..8666
                     /gene="pdxA"
                     /locus_tag="VFR00_00345"
     CDS             7596..8666
                     /gene="pdxA"
                     /locus_tag="VFR00_00345"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003567733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HEU0157723.1"
                     /translation="MSRMTFDSHKSAEFPGVGTPIIAITMGDAAGIGPEIIMRTFAHD
                     EVYRRCRPFVVGDARRLQLAGECVGSPLTIKTLADPAEARFAPGTVDCLDLGLIPVGL
                     PWGQVSAIAGNAAYQYIERAVALAMAKRIDAICTAPLNKEALHAGGHAFPGHTEILAA
                     LTGTPEVSMMLMTPKLKVIHVTAHIGLLDAIAKIEPGLVERTIRRGHAALQGAGHSAP
                     RIGVCGINPHAGENGLFGYGEEETKIAPAVRNLVASGLAVTGPLPADTLFFRAQRGDF
                     DLVVAMYHDQGHGPIKVMGIDTGVNVTVGLPVVRTSVDHGTAFDIAGTGKADEASLIE
                     AINQAVSLTAAAVPSAGAALTS"
     gene            8719..9864
                     /locus_tag="VFR00_00350"
     CDS             8719..9864
                     /locus_tag="VFR00_00350"
                     /inference="COORDINATES: protein motif:HMM:NF017532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HEU0157724.1"
                     /translation="MATSVDYGAEETAMQAYLRAGEAKAFALGNRGPIRFDENGRLAP
                     DILAAYWRCGFYVFEGVLVPEELAHIEADVKEILERLPREKGAVLDKHGRPALAADCK
                     APTLFWSKPLGDPFGGTSLANGRHPVKMFEPAAAADAPREVVYLILGSLQFSEACLRV
                     YGHPGLLAVAAAINGDDFVPFNEALFIKAPGLGASVAWHQDGTTHWDAPNWDEGTHGF
                     NFMGQLYGSTAANGVWVVPGSHKLGKIDIKRWVAEAGSERLPEAVPIIAKPGDVAITN
                     RQALHGSFANTSKDWRVTVNMGFHRRRSVLGVEAGGVHNARSVYDEARIAERAKMIGY
                     GIDARRRRFPRERAFAYRPHADAGVSYAWTAESKASIKDYNLLDLSI"
     gene            complement(10015..10800)
                     /locus_tag="VFR00_00355"
     CDS             complement(10015..10800)
                     /locus_tag="VFR00_00355"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEU0157725.1"
                     /translation="MRWWIVGFALLALACPPAALADGTPEAPFAWTGLYVGINGGYSW
                     GKVSNHLKASSTPGSSSAASISADLDGPIGGGHVGYSWKSQRWVYGVEADFLGSAEDG
                     GISGCSQFRCTTISYGLDWLGTLRGRLGYLLEPRLLLYATGGLAYGHISTDFLSTSAP
                     TGAAIFNDRPQVASDSTTKAGWVVGGGLEMALDRNWMLRAEYFYADLGTIASKSSTSV
                     SASPTGPQCLVETKETLRTDLVDQIFRIGLSYRFAADTYVPLK"
BASE COUNT         1779 a         3672 c         3720 g         1892 t
ORIGIN      
        1 atcagaagct cggtgctgaa gatcaccggc gccccttcct ttacgccgcc tcgccacggg
       61 caggctgtgt ttgcgcgcgc ggccttctcc gcctcaggcc ttcgcgccac ttgggactat
      121 tcgcccagaa cgtaggccac ggtggcggtc gccggcgtct gatccttggc ggcatagttg
      181 agcaccatca acggcggcga ccagttgtgc cacaaggcgc cctccgcacg cgggaaggtg
      241 atgggtgtct tcgaccagcc gctgaacgag ccctcctcca gcgccttgat gatcgccttg
      301 ggctcggtcg acttcgcctt ttcgatcgcc tcggcgaaca ccaaggcgtt ggcaaagccg
      361 ttcagcgccg catagaccgg cggctcgttg aattttgcct ggtagcgctt ggcgaactcc
      421 ttgccgacct cggaaagctc cgacttcggt gaatagaagg ccacgaagtt gatgccgacg
      481 cccttgtcgc cgtgcagttg ccagaactgt ggccgcgccg gggcgtcata ggacgtcacc
      541 atggccgccc ccggcaatag accaacggtg tgggcctgcg taatcatcag gtcgagcggc
      601 tggccgacac cgacgttgac gatggcgtcg ggcttgaacg ccttgacctg caagagttgc
      661 ggcaagaggt cggtcgtggt gcgatcgaac ataatggtct ggatctcgag gccgagagcc
      721 ttgtccttgt tctgcttctc ggtttccttg gcaagaccga tgccgtagtc cgtggtctcc
      781 gccaggatgc tgacccgctt gtagttgttt tttttgatcc agccgagcca ggtcgccgcg
      841 cgcggcgggt cgaccgcgtg ggtgcggaag gcgacgtcgt aatgcttggc ggtgacgtcg
      901 ctggcgccgg tctgcgtgcc catggtcggc acgcccattt ccttcgccac ttcgttgacg
      961 gcgatattgg cgcccgaatg aataaagccg aacaccgcca ccgccttgtc gttcgacacc
     1021 agccggcggt aggcggccac ggcctgctcg ttcttgccgg cgctgtcctg caccgagagc
     1081 gcgaccttgc ggccgagcac gccgcccttt tcattgatgt actcggcacc gatctccgca
     1141 ccgcgcttgg caagctgccc gagcgccgtt tcgccgggtg gcgacagcgg cgtgatgacg
     1201 ccgaagacga tcggctcctg cgccgccgct gctcccagtc cgaacagaac cgacaacagc
     1261 agggctccgc ctgcacatcc ccctcgccac atgctgcgcc tcctcggtcc tcgttcactg
     1321 ggcgtccagc tggcgtgccg ccacgaagcc cgcgcagcgc tgcccacccc ccgcatactg
     1381 ccgtggcgaa cggcaccgtg tcgaggccat cccgtgtcaa aggggccgcg ctagcaggcg
     1441 gtcgcgcagg cgtgcaaatt ctaaggtgac cggctccagg tcatcgacat gaaagcccgc
     1501 ggccagggcg gggctttccg cctcgagccg atcgcgtaag cgcccggcat agtggacccg
     1561 tccgtcggcg gcaacggaga gcccgttttc ggcctcatag cgggcgttcc aggccacggc
     1621 gtcggtcggc gataggccgg ggggcaagtc gagcgcgagc cgcccgttgc taatggcaac
     1681 ggggtagccg ccgggcaaac cgaccggccc cggcacgtga ccgcgccagt cgcggcccgc
     1741 ggccagcgcc agcatcagcg tcacaccgct ggcgccggaa atatcgatca cgggctccgg
     1801 cgtcagctgc acctcggcga agcggtcaaa gacgtcgccg atttcgcggc cgtcgagcca
     1861 gacccgcgca gcggggcccg cccgttcggc gggcgggcgg cgccaggcac cgatgttctg
     1921 gtagtgacag agcagcttga ggtctggcgc cacccgagcg gccgcgagac tgccggcgaa
     1981 ggcgtgggcg agaatggcga tattgccgat gccgcacaaa accggcaatc ccatgcccat
     2041 cacaatggca ttcgccacat cgggaaagca gcaattgatg agctggctct tgagccccag
     2101 ctcggctgcg gcgcgggcga tgcgcgaggt caaaagcgcc tggaagaccg ccgtcgcact
     2161 gaggcctccc tcggccacca ggcgggtcca ggcattgccg cccgcggcga tcacggcgcc
     2221 ggcctggacg gaggccgctt gcacgatcac ggcgggctcg agtttggcga gcaccgtgcc
     2281 cgccgcaccg ccggcggcaa ggtcgacctc gacggtggca agctcgagcg ggcagccgaa
     2341 catatgcgcg cgggcgcggg cggccgtggc gatccaggcc aaccgggccg gattgcggcc
     2401 ggcgatggcg acgctaaccg gctctttgct cgcgctcgcc aaatcgcaag caatgcgctg
     2461 ggcgaagctc ccgctgccaa aaatgaggat gtcgcaggcc cgcgccatgt gctcttctcc
     2521 actcctaaga agcctgattt gatcccattc ctggggtcct tgtcagcgct tgttctcaag
     2581 ttgggcgccc agagggacga tggtcagcgc tagcgccgac gtggcgatcg ttggtggcgg
     2641 ccttgtgggc tgctttgccg cctattttct gcgcgcccgc gggcgctcgg tcatcatcct
     2701 cgaaaagggc gcggcggctg cggcggccag cggcagcaac ttcggcaact tgcgcctgca
     2761 gggccgccat cccgcgcaat ttccgctgtc gctgcgggcg caggcgatct gggaagacct
     2821 tgcccgcttg agcggggagg cgtgcgggct ggttgcctgc gggcatctct atctgggcct
     2881 ggccgcgagc gatcagccga agctggcgac cgccgcgggc gcggcgctcg ccgccggcct
     2941 tgacgttgag ctcttgacgg gtgcggcggc gcgacaccgc tggcccatgc tctcaagcct
     3001 ggtgaccggc gcctcctggt cgaagcgcga cgccgtggcc gagccggggc gggttgcccc
     3061 gatcgtcgcc cgcctggcgg tgcgcgcggg cgcggagctc atcgagcaca ccaaggtggt
     3121 gcgcattgag cgcgcggcag ggggatttgc catcggcacc gatgggggcg cgaacatccg
     3181 cagtgactat ctcatcaacg ctgcgggcgc ctggggcggg gagatcgcgg cggcgtacgg
     3241 cgagccggtg cccctgtttg ccgccgggcc accgctattt gcgctccggc cggagcaccc
     3301 ctgggagggg ccctccctcc acgccatcga cgggaccgtg ctgttgcggc agggcgaggg
     3361 cggcgcggcc gtggccggcg cctttccgcg cgttgcggcc gatctagcga gcggtgtggc
     3421 ccgcgtgccc agcgatcgct tggcgcgcgg ccttgagcgc ctcggcgccg tgcttccggg
     3481 cctcggacgc ctgctgccgg gccgcgtctg gtcgggcgtc gagggctacc tgccggacat
     3541 gttgcccatc atcggctgga gtggaagcac gccggggctc gtccacgcct tcggcttttc
     3601 cggtcacggc ttccagctgg cccccggcgt cggtgcggtc ttggccgagc tgattgcgac
     3661 cggggtgacc gagacaccga tcgaggcctt ttcgctggca cggttcggcg gcgaggtggc
     3721 gtttgacgac aagctttggc acgaattcga tgccgagctc gtcgcgcgat ttcgcaagca
     3781 agccgcgcca gacgttgagg ccggcgggcg atctccctga gcaaagggca cgatcgccca
     3841 gtcgcgtccg cggcgagggg atggctgtga ggagcgaggt ggagacccag caacgatgag
     3901 caacgagcga tcggtttttt tcgacatcag cgatggtctg gccatgctca ccctcaacaa
     3961 accggccaaa ctcaacgcct gggacacgcc catgcgggcg gagattgcgg gcctgttgcg
     4021 ggagtggaat gtcgatcaac gcgtgggcgc ggtgatcgtc accggagcgg gggatcgcgc
     4081 tttttcggcg ggccaggatc tcgacgagac ggaaaagttt cagtcgggca gtgacggggc
     4141 caactggttt cagtcctggc gcgacttcta taactccatt cgcaatctcg ataagccctg
     4201 cctggccgca ctcaacggcg ttgccgcggg ctcggccttc caggcggcca tgctgatgga
     4261 cgtgcgcatc ggccacagcg gcgtgcgcat gggtcaggcc gaaatcaatt ccggcatccc
     4321 cagcgtcacc ggcccgatgc tgatgctgcc gcgcatcggc ctgtcgcgca ccgtcgaact
     4381 gacgctgacg gggcgcatga tggatgccgc cgaatgccac gccgtcggcc tcatccacta
     4441 tctcgtcgag cgccccgagg acgtcttgga aaagacgcgc gaggtggccc gcttgctggc
     4501 cgccaagccg cccatcgcca tgcggctgac caagacgcga tttcgccagg tgacgcagcc
     4561 ggctttcgag gaggccttcg agaatggcag cgtctaccag gccgaggcat tcgcctcggg
     4621 cgagccgcag gcggccatgc gcgtgttctt tgccgagcgc gccgcccgac gcaaggcgcg
     4681 cgccgccggc aaggcatccg atgcgacctg aggcggccgt ggcgcaattg cgggcaccct
     4741 ttcccgacac ggacgccggt tttgtcgggc ttttcgacag ccaagcacgg gcaacgccgc
     4801 agcgcagctt tgccatcctc ggcggcgagg cgctgagctt tgccgcgctc gatcggacct
     4861 cgcgtgcgct tggcgcctgg ctgcgggcca aagggctggg gcctggtgac cgcgtcgccc
     4921 tgatgctgcg caacggcgaa accgcgctgt ccctgatgct ggcgctggcg cgcacgggca
     4981 gcatctgggt tcccatcaac acccaggccg tcggcgacgg tttggcctac gttctggggc
     5041 acgccgaacc gagcctgatc attgcggata gtgaccagct gccggcccta tccgcctgcg
     5101 gcgtcgcggc ggccgcgcgc gccattgcgg ttgaggatgc ggcccgcgag gcggcacgcg
     5161 cgccggcgag cgaagcgctt atggttgtgc cgccggcgga cgcccccttt gccatcatgt
     5221 acacctccgg cacgaccggc cgacccaagg gggtgctcgt atcgcaccgc atgctgcggc
     5281 tgtccggcga ggcggcggcg ctggtcgccg atccgagccc cggcgacgtg ctctaccttt
     5341 gggagccgct tttccacatt ggcggcgcgc agatgctggt cttgccgctc atccgcgatg
     5401 tgacgctcgc catcgccgaa cgctttagcg cgcgcggctt ctggacggac gtccagggca
     5461 agggggccag ccacatccac tttctcggcg gtattttgca aatcctgctc aagcagccgc
     5521 cgggaccttt ggatcgcggc cacggcgtgc gcatcgcctg ggggggcgga tgtccggtcg
     5581 acgtttggca agccttccaa gatcgcttcg gcgttgagat ccgcgaatgt tacggcatga
     5641 cggaatgctc cagcgtcacc accgccaacc tcgatgctcc gattggatcg gttggcaagc
     5701 ccgtgccgtg gttcgacgtc gccgtcctcg acgcggcggg acggccggtg gcccccggcg
     5761 tccgcggcga gatggtcgtc acagaacgcc tgggcggtgc gcttacccgc ggctacttca
     5821 aagatggcga tgcgaccgcc cgcgcctttc gcgcgcaggc cttcctgaca ggggatctgg
     5881 tgtcctacga cagcgatgga aattactatt tccacggccg catgacggac agcgtgcgcg
     5941 tgcgcggcga gaatgtgacg gcgctcgaag tcgagagcgt ggcgcgcacg catcccgccg
     6001 tggaggactg cgccatgatc ggtgttgccg cggaggtggg tgaagcagag atcaaactgt
     6061 tcgttaccct gcggcaaggc gtcgagctcg agccgcaaga gttatccgcc tggctggcag
     6121 accgtctccc ccgctaccag cggccccgct acatcaccgt tctcgatgcc ttcgagcgca
     6181 cgccgagcgc acgcatcatg aagcacgcac tgtcgcggcg aaccgatgat tgctgggatc
     6241 tcgcggccgt cgggatctaa ggcgacgcca atggcagcaa atgccgcccc gttgcgcctt
     6301 agcctcgacg gccgccatga acacgcaggg acgatgacca aaatcttgat ccttgccgat
     6361 gatttgtcgg gcgccgccga ttgcgccggg ggttgcgcag ccgcgggcct cgacaccctc
     6421 gtcttgctgg aggccgctgg cgccggacca gcgatggcga gcgtgctcgc gatcgatctt
     6481 aactcgcgcg acaaatcgcc ggccgctgcg ggtgaggcga tcgctcggac gatcgggcaa
     6541 atgcgcgacc ttggcgccaa gatcctctat cacaagatcg attcaaccct gcgcgggaac
     6601 tgggcccacg agttggtggc cgcgcgcgca agcctcgctg tcgcgcacgg ggcctccccg
     6661 ctcgccctcg tggttcccgc ctttccggcg cgcggacgca tcacccggtc gggccgcgtg
     6721 ctggtcacag cgtcgcgcct ccttggcgcc gaggcaagcg caccggcgcc catggtggct
     6781 gcaggcgagg tcgcagcacc cctgcgcgag cgcggtctca gcgtgcgcat ccttgctcgc
     6841 cctgaagtcg aggcgccggc ctggcgagcg gctggccttt tcgacgctat cgctcgttcg
     6901 ggtgtcgacg ccgtcgtcgg cgatgcggag agcgattgcg accttgcagc cattacggcg
     6961 gcggcgctcg ccgcgcacgt gccgttgctc tgggtcgggt cggcgggcct gatgcgagcg
     7021 ctggcgcaaa tcctaagcag aaaggcacct gcgccgccgg ccaggccacc cacggcgcga
     7081 ggccccctgc tttttgtcgt cggcagcacc tcggcggttt cgcgcaccca atttgagcgc
     7141 ctgattgcgg agcccggggt cgcaggcttc tgcgtctccc ccgaggaggt gcggggagcg
     7201 ccacgccggc tcgaggccta tctcgatggc gcgcgtgcgc gcggttgcga cgccgcgctc
     7261 aggctggtcg gcgacagccc gcttgccacg cctcacgacc cgagtttggt ctctgcgctt
     7321 gccgacatcg tcgggccgcg cttgcctggc tttggcgggc tcgtcgccac cgggggcgcg
     7381 accgcgcgcg gcctcatcga aaaggccggg tactcggcga ttgcgctcgg cggggaagtg
     7441 gaggccggcg tcccttgggg cctcgctttg agggacgcag cctcgggggg tataggattg
     7501 gaggggcgag ggctgcctct cgtcaccaaa gccggcggct ttggcgacgc ggacacgctc
     7561 cttgtctgcc gcaaggcctt gaaggtgagc gagacatgag ccgcatgacg tttgacagtc
     7621 ataaaagtgc tgagtttccc ggcgttggga cgccgatcat cgccatcacc atgggtgacg
     7681 ccgcggggat tgggcccgag attatcatgc ggacatttgc ccacgacgag gtctacaggc
     7741 gctgtcggcc ctttgtggtg ggcgatgcca gacggctgca attggctggc gagtgtgttg
     7801 gcagcccgct gacgatcaag acgctggccg atccggccga ggcgcgcttt gcgccgggaa
     7861 cggtcgactg cttggacctt ggactgatcc cggttgggct gccctggggc caggtttcgg
     7921 ccattgcggg caacgccgcc tatcaataca tcgagcgcgc cgtggccctg gcgatggcca
     7981 agcgcatcga tgccatctgc accgcccccc tcaacaagga ggcgctgcac gccggcggcc
     8041 atgcctttcc cggccacacg gaaattctcg ccgccctgac ggggacgccc gaggtctcga
     8101 tgatgttgat gacccccaaa ctcaaggtca tccacgtcac ggcgcatatc ggcctgctcg
     8161 acgccatcgc caagatcgag ccggggttgg tggagcgcac cattcggcgc ggccatgccg
     8221 cgctgcaagg cgcggggcat tctgcccccc gcatcggcgt ttgcggcatc aatccgcacg
     8281 ccggagaaaa tggcctcttc ggctatggcg aggaggaaac gaaaattgcc ccggccgtcc
     8341 gcaacctggt cgcgagcggc ctggccgtca cgggcccact gccggcggac accctcttct
     8401 ttcgcgccca acgcggcgac ttcgatctcg tcgttgccat gtatcacgat cagggccacg
     8461 ggccgatcaa ggtgatgggg atcgatacgg gcgtgaatgt taccgtgggt ctgccggtgg
     8521 tgcgcacgtc cgtcgatcac ggcaccgcct tcgatatcgc tggcaccggc aaggccgacg
     8581 aggccagcct gatcgaggcc atcaaccaag cggtgagcct cacggccgcc gctgtgccat
     8641 cggctggagc ggcgctcaca tcatgatttc ggcgccggcg atcgcgggcc gcggccggag
     8701 acggataggg aggagaggat ggcgacgtct gtcgactacg gtgccgaaga gacggcaatg
     8761 caagcctatc tgcgggccgg cgaagccaaa gcgttcgcgc tcggcaaccg cgggccgatc
     8821 cggtttgatg agaacggtcg actggcgccc gacattttgg cggcctactg gcggtgcggg
     8881 ttttatgttt tcgaaggcgt gctggtcccg gaagagttgg ctcacatcga agccgatgtg
     8941 aaggagatcc tcgagcggct gccgcgcgaa aagggtgcag tgctcgacaa gcacgggcgc
     9001 ccggcgctcg ccgccgattg caaggcgccg acgctgtttt ggtccaagcc gctcggtgat
     9061 ccctttggcg gcacgagcct cgccaacggc cgccatcccg tcaagatgtt cgagcccgct
     9121 gcggcggcgg acgccccgag ggaggtcgtc tatctgatcc tcggctcgct tcagttttcc
     9181 gaggcgtgcc tgcgcgtcta tggccatccc ggtctactgg cggtggccgc cgccatcaac
     9241 ggcgatgact tcgtgccttt caacgaggcg ctgttcatca aggcgcccgg gctcggcgcc
     9301 tcggtcgcct ggcaccagga cggcaccacg cattgggacg cgccgaactg ggacgagggc
     9361 acgcacggtt tcaacttcat gggccagctc tatggctcga ccgcggccaa cggcgtgtgg
     9421 gtcgtgccgg gttcccacaa gctcggcaag atcgacatca agcgctgggt ggcggaggcc
     9481 ggcagcgagc ggctgcccga agcggtgccg atcatcgcca agcccggcga tgtggcgatc
     9541 accaaccgcc aggcccttca cggctccttt gccaatacca gcaaggattg gcgagtgacg
     9601 gtgaacatgg gctttcatcg ccgccgctcg gtgctgggcg tcgaggctgg cggcgtgcac
     9661 aatgccagaa gcgtctacga tgaggcgcgg attgccgaac gcgcaaagat gatcggctac
     9721 ggcatcgatg ccagacgacg gcgctttccg cgcgagcgcg cattcgccta tcggccgcac
     9781 gccgatgcgg gggtgagcta cgcctggacg gcagagagca aggccagcat caaggactat
     9841 aacttgctcg acctcagcat ctgacgccgt tgcgacagcc gtgggcgtcg ccagcccgaa
     9901 attctcgtag ttgggcgatc tgaaaaaccg accggcgccc cggccgaact agctcggagg
     9961 gcgcccttgc ttggatttga gctgctcgag gggtgcagct ctttgcaaag gcgcctactt
    10021 tagcggcacg taggtatccg cggcaaacct gtagctcagt cctattcgga agatctgatc
    10081 aacaaggtct gtacgcagcg tctctttggt ctccacgagg cattgcggtc cggtagggga
    10141 agcgctcacg ctcgtgctgc tcttgctcgc tatcgttccg aggtccgcat agaaatactc
    10201 ggcgcgcagc atccagttcc gatcgagcgc catctccagg ccaccgccaa caacccagcc
    10261 cgccttcgtc gtgctgtcac tcgcgacctg cgggcggtcg ttgaatatcg ccgctcctgt
    10321 gggcgcggat gtcgaaagaa aatcggtgct gatatggcca taggccaagc cgccggtggc
    10381 atagagaagc aaacgcggtt ccagcagata accgaggcgc ccgcgcaatg ttccgagcca
    10441 atcgagcccg tagctgatgg tcgtgcaacg aaattgcgaa cagccggaaa tgccgccgtc
    10501 ctcagcgctg cccaagaaat cggcctccac gccgtaaacc caacgctgcg atttccagct
    10561 gtagcctacg tggccaccgc caatcgggcc gtcgaggtcg gcgctgatgc tagcggcgga
    10621 acttgaaccc ggagtcgatg aagccttcag gtggttggaa actttgcccc aactgtaacc
    10681 gccgttgatg ccgacataaa gacccgtcca agcaaagggg gcttcaggcg ttccgtcggc
    10741 cagcgcagca ggcgggcagg ccaaagcgag caatgcaaat cctacgatcc accaacgcat
    10801 agtgaaatcc acaaaacaac acagcgagcc gctgacctta gccaatctcc acaagactgt
    10861 atgtgtcgcg atggcaactt gcctcttgaa accgcgcgaa ccacgttagc tcgcgtagtg
    10921 ccagcccatc ggcgcccccc caaatcggct cgccaagcgg cgcgccaaca aaaatgcctc
    10981 gatggcgtct actgagctcg cactcgctca gcaattctct acgacgcacc caaatctcga
    11041 agtcgtcggc agcgccgcgg caa
//
LOCUS       DASTMT010000016         6719 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_6238435, whole genome shotgun sequence.
ACCESSION   DASTMT010000016 DASTMT010000000
VERSION     DASTMT010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 6719)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6719)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6719
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6238435"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(366..779)
                     /locus_tag="VFR00_00360"
     CDS             complement(366..779)
                     /locus_tag="VFR00_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020180244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU0157726.1"
                     /translation="MAVTPYLFFDGRCEEAIEFYKKALGAEVQMLMRFKDGPDKSMCA
                     PGTEEKVMHACLRVAGMPVMLSDGRAQMQPKFEGFALSLDAKDEADAERLFGALANGG
                     QVQMPLTETFFAKRFGMVADRFGVGWMLLAGAKNA"
     gene            complement(817..1317)
                     /locus_tag="VFR00_00365"
     CDS             complement(817..1317)
                     /locus_tag="VFR00_00365"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEU0157727.1"
                     /translation="MAGKMAGKANLDLTPDPSAIVTVRELAAPRELVFDVWTDPKHLA
                     QWWGPYGFSTTTSAFELSEGGVWRFVMHGPDGRDYQNRITFDEIVRPERIRYHHGGGD
                     DAEPVEFRTTVTFEDLGHGRTRLTLHAQFPSAAERDRVIKAYRADQGAIETLSRLADY
                     LAQLAA"
     gene            complement(1375..1719)
                     /locus_tag="VFR00_00370"
     CDS             complement(1375..1719)
                     /locus_tag="VFR00_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018063683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEU0157728.1"
                     /translation="MAADPLSATFAALADPTRRAMLARLSRGEMSVNELAKPFKISGP
                     AVSKHLKVLERAGLITRARAAQRRPCRLAPSALRTIDAWLADYRQLWETRLDRLDAYL
                     SRLQQKPPTPRR"
     gene            1919..2314
                     /locus_tag="VFR00_00375"
     CDS             1919..2314
                     /locus_tag="VFR00_00375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0157729.1"
                     /translation="MRSSHPRLRVFFLLLVALLLAGHPWLRATAPLTASSDPLAADLQ
                     FALCSLHGGMRGGDGVPVQDRETPTCPWCALGTGPGPHLTAITAVPIDVLRPPRTLYA
                     ALVALATPPPPPFGAWLAPPPRAPPRPSA"
     gene            2704..3078
                     /gene="copC"
                     /locus_tag="VFR00_00380"
     CDS             2704..3078
                     /gene="copC"
                     /locus_tag="VFR00_00380"
                     /inference="COORDINATES: protein motif:HMM:NF033814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis periplasmic binding protein
                     CopC"
                     /protein_id="HEU0157730.1"
                     /translation="MLRRSRLLAALLACLGVVFGGAVAAHTHLKTSVPAAGASLAEAP
                     TEIRLKFHDAIEASLSHVGVETNTGEPVPAGVAASAAGDKTLLVLRLLQPLKPGTYKV
                     SWRVIAADTHKMRGSFSFQVRP"
     gene            3336..3704
                     /locus_tag="VFR00_00385"
     CDS             3336..3704
                     /locus_tag="VFR00_00385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0157731.1"
                     /translation="MLDKFNAQSLVETHTSRDRTAFELTFVDAQGARHTLRLSRGVAK
                     DLVPVLQSLTDHNGEGANGLTKMPKTCQVGHATYERLVLIKFDDDPPYAIGLEDARSL
                     WRDLRQESQTVSRRKRRALQ"
     gene            3853..4311
                     /locus_tag="VFR00_00390"
     CDS             3853..4311
                     /locus_tag="VFR00_00390"
                     /inference="COORDINATES: protein motif:HMM:NF016221.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016531 - copper chaperone activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HEU0157732.1"
                     /translation="MLNRLALLLLLCLAPGAGTLLAHDHGPSAIEIVQPWAPAMTGQD
                     PQLVYLTIRNRGNVPDRLMRALSPVAAKVELHAAGSGESEPKPVDGLSIAAGQDLVLN
                     PSGPHLAMVGLKRALLENQTFKLALEFDGAGRIVVDVVVGPPAASQPHRH"
     gene            4489..4812
                     /locus_tag="VFR00_00395"
     CDS             4489..4812
                     /locus_tag="VFR00_00395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0157733.1"
                     /translation="MRWGLIFAVLVLAGCAAEKYSDRYYSAERPPPDVRRISPVVYAD
                     EDCPPPQVIAAYCGSMRGRDACLAEPQCAWVGGDCRGIACRIPVPRRRYYSRDYRDYR
                     EYREY"
     gene            complement(4875..5039)
                     /locus_tag="VFR00_00400"
     CDS             complement(4875..5039)
                     /locus_tag="VFR00_00400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0157734.1"
                     /translation="MSRADRGFALFLGVMLVVLVTLFYELFPRHDPEGQSGGGASAPA
                     TTPAAPAPPK"
     gene            5749..>6719
                     /locus_tag="VFR00_00405"
     CDS             5749..>6719
                     /locus_tag="VFR00_00405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0157735.1"
                     /translation="MQPEAIARFRRGRTWLKPLALALAFAFLPPGPPARADAPIKRLL
                     IVYPDSSESHAAVVASEAVRSRLSHLASALTVQTHTEFLDRTQFPGQEHKDRFVRYLI
                     DKYSGAKPDVLLAFGADSLDAVTSYRKALGTTIPIVFCVLPPAGATVKVEPDMAGIVS
                     EHDVTKTVALARALQPDARGLLVVAGAADFDRKGALIAQQQLVDVEKQLKTRYLVGLP
                     DNTVQRELASVPRNTIILLLSKLTDAAGHGDAAALIAAANAPVYAPYSTYFGRGIVGG
                     YMDTFEGMGAQTADLVLGIFNNPVPPMLGPIKSTTQAFRVDARELER"
BASE COUNT         1124 a         2342 c         2080 g         1173 t
ORIGIN      
        1 ccaacgctcc ggccctcaag aacttcctac gcgacaagga cttggcgtcg gtagtcatcg
       61 gttgtttcct cctgattatt tttgcgactt ttgtatcggt tttgtatcgc aagcgctggt
      121 ataccacggt cccctctgcc gaaggaatgc ctcatttacc aaggcgcgag aatggaaaag
      181 ccggcgacgc aggcgcgccg cacgcgaagt gccgcgccga gggccggccc cggtcgcgcg
      241 ccacgccttg ggtagcgcaa aatcgcgacc cgagtgccgc tcacccaccg ctcaggcgcg
      301 gcaaggttgg gcagccgtgc gaaggacttg cgcgccgtag ctgccccgcc cgaggctatc
      361 cttcctcaag cgttcttggc gccggccaac agcatccagc ccacgccaaa gcggtcagcc
      421 accatgccga agcgcttggc aaagaaggtc tccgtgagcg gcatctgcac ctgcccaccg
      481 ttggcgagcg cgccaaacag gcgctcggca tccgcttcgt ccttggcgtc gagcgacagg
      541 gcgaagcctt cgaacttggg ctgcatctgg gcgcgaccat ccgacagcat caccggcatg
      601 ccagcaaccc tgaggcaggc atgcatgacc ttctcttcgg taccgggcgc gcacatgctc
      661 ttgtccggac catccttgaa gcgcatcagc atttgcacct ccgcgcccag cgcctttttg
      721 taaaactcga tggcctcttc acagcggccg tcgaaaaata ggtagggtgt gactgccatc
      781 acgttctcct ttttcgtcta gcgcgaggcc gcgacgctac gcggccaatt gggccaaata
      841 atcggcaagg cgcgacaacg tttcgattgc gccctgatcg gcacgatagg ctttaatcac
      901 gcggtcacgc tccgccgccg aggggaattg ggcatggagc gtcaggcggg tgcgaccgtg
      961 cccaaggtcc tcgaacgtca cggtcgtgcg gaattcgacg ggctctgcgt cgtcgccacc
     1021 gccgtgatga tagcggatgc gctcgggccg cacgatctca tcaaaggtaa tgcgattttg
     1081 atagtcgcgg ccatccggtc catgcatgac aaagcgccac acgcccccct cgctgagctc
     1141 gaaggcactg gtggtggtgc taaagccata gggcccccac cactgggcca gatgcttcgg
     1201 gtcggtccaa acgtcaaaca ccagctcgcg cggcgccgca agctcgcgca cggtgacgat
     1261 cgcgctcgga tccggtgtca gatcgaggtt ggctttgcct gccattttgc ctgccatggg
     1321 ggcatgctcc tttctgctcg ttcgaaccgc tgggaaagct ggggccggcg tagctcagcg
     1381 ccgcggggtc ggcggttttt gctgcagtcg gctcaagtac gcatccaggc gatcgagccg
     1441 cgtttcccaa agctgccggt aatcggccaa ccaagcatcg atcgtgcgca gcgcgctggg
     1501 cgccaggcgg cacggacgcc gctgggctgc gcgcgcgcgg gtgatgagcc cggcacgctc
     1561 gaggaccttc aagtgcttgg agacggcggg gccgctgatc ttgaagggct tggcgagctc
     1621 gttgacgctc atctcgcccc gcgacagacg cgcaagcatg gcgcgccgcg tcgggtcggc
     1681 caaggcggcg aaagtcgcac tgagcggatc ggcggccata attaacccat aggttatata
     1741 accgatcggt tagatactca gcacaacttc gcctgtcaag gccgggccgc ggcgcgccta
     1801 ccgtccgcgc aggcctcgac agcggcagcg ccggcgtctc ccggcaggcc aaaagagcct
     1861 accggcgtct tgcctaggtc cgattgaccg acacctagca atgtggccac ctcttcgcat
     1921 gcggtcgtca catccacgcc tgcgcgtctt ctttctcttg ctcgtcgcct tgctgctggc
     1981 gggccacccc tggctcaggg caacggcccc cctcactgcg agcagcgacc cgctggcggc
     2041 cgacttgcaa ttcgcgcttt gctctttaca cggaggaatg cgcggcggcg acggcgtccc
     2101 ggtccaggac cgggagacgc cgacctgtcc ctggtgtgcc ctgggcacgg gccccggccc
     2161 gcacctcacc gccatcaccg ccgtgccgat cgacgtactg cggccgccac gcacgctcta
     2221 tgccgcattg gtggcgctgg cgacgccgcc tcccccgccg ttcggagcct ggctggcacc
     2281 accaccgcgc gcaccgcccc ggcccagcgc ttagcgccgg accttctttt cttcctggct
     2341 gcgcaggcgc agcactggat cgctggcccg gccgaaccgg gatgacgcac cttctgcggc
     2401 cgtcgtcccg cccggcccca tgccccgcgc accccgtgcg gcccaaggcg ctcgccgccc
     2461 cacaatcgtt tcgatggaga ctgacatgca aaggctcgtt ttagccccgc tcagcgcact
     2521 tttcttcctg acttgggccg ttccggccct cgcgcatggc ggagcaccgc atcccaaatg
     2581 caagccgggc tatgcccttg acgccgagca taagtgcgtc aaggcaccca aaaaataagt
     2641 ttgcgcgcca gcagcccttg gtttcgccgg cggctgctgg cgccaatccc gacgaggtcc
     2701 tccatgctgc gtcgctcacg cctgctggcc gcgcttctcg cttgtctcgg cgtcgtcttc
     2761 ggcggcgccg ttgcggccca tacccacttg aaaacgtcgg tgcccgccgc cggcgccagc
     2821 cttgccgagg cgccgaccga aatccgcctc aagttccacg acgccatcga ggccagcctc
     2881 tcgcacgtcg gtgtggaaac caacacgggc gagccggttc cggccggggt ggccgccagt
     2941 gctgctggcg acaagacgct cttggttttg cgcttgctgc aacccctcaa acccgggacc
     3001 tacaaagtca gctggcgcgt gattgccgcc gatacgcaca agatgcgagg cagcttcagc
     3061 ttccaggtcc gaccttgaga tggcgccgcc tcgaaatggc ttggcctccc gctggtgcgc
     3121 tcgcggcccg tcggtcgcgc tgccttgtcc ggttcgcccg tccgctgcgc ttcgccgtgc
     3181 ctgccggcgc gccaccgccg cgccagcctc cgccgcggcg ccgcctgccc ggcggccaaa
     3241 gttcatcatc tcggcccttg agcgtggccc atccgtcctt gtgcccggcc cgattcgaga
     3301 tccatcatgt cagcctgggg tcatgggggg ctgtcatgct ggacaaattc aatgcgcaat
     3361 cgctcgtgga aacccacacc agtcgcgatc gcacggcgtt cgagttgacg ttcgtcgacg
     3421 cccagggagc gcgccacacc ttgcggctct cgcggggcgt ggcgaaggac cttgtgccgg
     3481 tgctgcaatc cttgacggat cacaacggcg agggcgccaa tgggctcacc aagatgccca
     3541 agacctgcca ggtcgggcac gcgacctacg agcggttggt gctaatcaaa ttcgacgatg
     3601 atccacccta tgccatcggc ctcgaggacg cccgctcgct gtggcgcgac ctgcgtcagg
     3661 aaagccagac cgtctcccgc cgcaaacgcc gcgctttgca gtaaaagtgg gcttaagtcc
     3721 cagcccccgc ggcgccagct gccgctgttt ttggcgcttg cgcggcgagc cggcataaag
     3781 aacgatcgag gcgatggctt ttgcggcttg cgtcacctcc tcgcccaatg gccttgccgg
     3841 gagtggcccc tcgtgctgaa ccgccttgct ctgctgctgc tcctttgcct tgcgcccggc
     3901 gccggcaccc tgctggcgca tgaccacggt ccgtctgcaa tcgagatcgt gcaaccctgg
     3961 gccccggcca tgaccggcca agacccgcag ttggtgtatt tgaccatcag aaaccgggga
     4021 aacgtgcctg accgtctgat gcgcgccctg agcccggtcg cggccaaagt ggaattgcac
     4081 gccgcgggct ccggcgaaag cgaaccgaaa ccggtcgatg ggctcagcat cgccgccggc
     4141 caggatctcg tgctgaaccc cagcgggcca cacctcgcca tggtcggctt gaagcgcgcc
     4201 ctcctcgaaa atcagacttt caaattggcc ctggagttcg acggcgcggg acgcatcgtc
     4261 gtcgatgtgg tcgttgggcc acccgcggcg agccagccgc accgtcacta gacgcgcgtg
     4321 cgggcgggcc ggccacagtc ggcacaaatc cagtccgtgc acggtgttac aggcgccgat
     4381 cttgcccaca gctttcgcac gagaaggccc gctgtggctt gtctgtcact agccaatcct
     4441 gcccgctgac agtatccttt cccatcgcga cgtgttgagg gatgcgtaat gcgttggggg
     4501 ctcatcttcg cggtgttggt gctggctggc tgcgccgccg aaaagtattc cgaccgctac
     4561 tactccgccg agcgtccgcc cccagacgtc agacgcatct cacccgtggt ctatgccgac
     4621 gaggactgtc cgccgcccca ggtgattgcc gcctactgcg gcagcatgcg cggccgcgac
     4681 gcctgcttgg cagaaccgca atgtgcctgg gtcggcggcg actgccgcgg catcgcctgt
     4741 cgcatccccg tcccccgtcg ccgctactac tccagggact atcgggacta cagggagtat
     4801 cgggagtact gagctcacac gctgcgctgc gccacgggcg ctggacatgc gggagaggtg
     4861 tgcgccgacc gacattattt tggtggcgcc ggtgcggctg gcgtggtcgc cggcgccgac
     4921 gcgccgccgc cgctttgccc ctccgggtca tgccgcggaa agagttcgta aaaaagcgta
     4981 accaacacga ccagcatgac acccaagaac agggcaaagc cgcgatccgc acgagacatg
     5041 tccggcagtc ctcctcagcg tggcgggcgc tcggccgacc ggggccacac cacctgaaag
     5101 ctcggcgcga cggccttgag cgacagagaa cctggcccgc ccgcgcttgc tgttccacgt
     5161 ggccttgagc gcctcggccg acggtcttaa cccgccaggc atgaatgcgg catcggcggc
     5221 aaaagttccg accccacggg cgagcccggg gcacgcgccc ccggccagca gcgccggcca
     5281 agccacaaag caagcgggct tgccaacccc gcttctggcc agccataggg cgggccgaga
     5341 ggcttgcgcc ggcgctcggc cccccaactg ccgccgcgcg ccggccggcc ccgctttggc
     5401 ccctttggcc aaggcccgcc aaggccgccc tgccggcgga aaggcgtggc ttagcgtggc
     5461 gcgtcggcgc cgcagggcgc gccatggccg caggtgcggc gtgcgtcccc agcatcccgg
     5521 gaccagcgct tttgctccgg cgagcgcgtc tcccaacgct gtagagggca acgctgtaga
     5581 ggggggtggg cgcgccgcgg cgcgttcgct gccattaact gagctcacca gactacagag
     5641 cttctggatc acatctcagt atattaccgg atgtcctaag ctttccgacg gcaatctatt
     5701 cgaaaatcgg atcccaccta accgcagtat cgaaaaggag cgggccatat gcagcccgaa
     5761 gcgattgcgc gttttaggcg cgggcgaacc tggctcaagc ccctcgcgct tgctctcgca
     5821 tttgcattcc tgcctccggg cccaccggcg cgcgcggacg ccccgataaa aagactgctg
     5881 atcgtttatc cggatagcag cgaaagccat gccgccgtgg tggcgagcga ggcggtgcgc
     5941 agcaggcttt cccacctggc ctcagcactg accgtgcaga cccataccga gtttctcgat
     6001 cgcacgcagt ttccaggtca ggagcacaag gatcgcttcg ttcgctacct gatcgacaaa
     6061 tacagcggcg ccaagccgga tgtgctgttg gcttttggcg ccgattcact ggacgccgtc
     6121 accagctacc gcaaggcgtt gggcacaacc attcccatcg tgttctgcgt gctcccgccg
     6181 gcgggcgcca ccgtcaaagt cgaaccggat atggccggca tcgtcagcga gcacgacgtc
     6241 accaaaacgg tggcactcgc gcgcgccttg cagcccgacg cccgcggtct cctcgtggtg
     6301 gccggcgcgg ccgactttga ccgcaagggc gcactgatcg ctcagcagca gctggtcgac
     6361 gtcgaaaagc agctgaagac gcgctacctc gtcggtctgc ctgacaatac ggtgcaaagg
     6421 gaactggcga gcgtgccgcg caacacgatc attctgctgt tatcgaagct gaccgatgca
     6481 gccgggcacg gcgatgccgc ggcccttatc gccgcggcta atgcgccggt ttacgcgccc
     6541 tatagcacct acttcgggcg cggcattgtc ggcggctaca tggatacctt cgaaggcatg
     6601 ggggcgcaga cagccgatct ggtgctcggc attttcaaca accctgtgcc cccgatgctg
     6661 gggccgatca aaagcacgac ccaggccttt cgtgtcgatg cgcgggaact tgagcgctg
//
LOCUS       DASTMT010000017         3741 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_2304644, whole genome shotgun sequence.
ACCESSION   DASTMT010000017 DASTMT010000000
VERSION     DASTMT010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3741)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3741)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3741
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2304644"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(224..616)
                     /locus_tag="VFR00_00410"
     CDS             complement(224..616)
                     /locus_tag="VFR00_00410"
                     /inference="COORDINATES: protein motif:HMM:NF014534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPF/RaiA family ribosome-associated protein"
                     /protein_id="HEU0157736.1"
                     /translation="MNAPVEVNFHGIQRSEAIEQRVRDKVAKLEKHFERMTSCRVVLE
                     ATQRTALKPKVYCIKIEIGVPRQRPIVVCHERVGSHASEELTMALRDAFEAALRKVDG
                     TASKRGQRSRLERGRRRPNATDRGPATA"
     gene            complement(727..2163)
                     /locus_tag="VFR00_00415"
     CDS             complement(727..2163)
                     /locus_tag="VFR00_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012029768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HEU0157737.1"
                     /translation="MGKITGFMEFERHDRGYATVEERLKHWREFVVPLSDADTRVQAS
                     RCMDCGIPYCHNGCPVNNQIPDWNDLVYREQWRTAARNLHSTNNFPEITGRVCPAPCE
                     AACTLNIDDTPVTIKTIECSIADKAFAEGWVVPEPAEKKSGKKVAIVGSGPAGLAAAQ
                     QLARAGHEVHVYDKHPTPGGLLRYGIPDFKMEKSIVERRIKQMEAEGVVFHSNVHVGR
                     DLPAQRLESEHDALLLAAGSEQPFDFFAKSPGRELDGLVFAMTFLPQQNRRVAGQPPQ
                     DEAQITAQGKHVIVIGGGDTGSDCIGTSFRQGAKSVTQLEIMPQPPVKENKALSWPHW
                     PLKLRVSSSQAEGAQTEYSISTTGFAGRNGKVEKLLYTRVDATLKPIAGKEGELPADL
                     VLFAMGFSGPVESDLVAELGLSVVPRGRFKGLDANERDYRIKGKPKLFAAGDVRRGQS
                     LVVWAIREGRQAAHAIDSFLMGRSDLAR"
     gene            complement(2178..2786)
                     /locus_tag="VFR00_00420"
     CDS             complement(2178..2786)
                     /locus_tag="VFR00_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015927875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEU0157738.1"
                     /translation="MKIYGDLGSGNCLKVKYTADLLGLAYAWVPVEVLKGETTTPEFV
                     AKFPMAQIPAVEFDDGRCLAQSNGIVRYLAQGSPLLPTDPFVAAKVDELLFWEQYSHE
                     PYLAVCRFHMVYLGRPKEAREPQRVERGEKALDFMDAMLKGRRWLVGDNLSIADLVLL
                     PYTRLAPEGGFELCQRPDLRAWIARCEGALGLTADAPSRALN"
     gene            complement(2837..>3741)
                     /locus_tag="VFR00_00425"
     CDS             complement(2837..>3741)
                     /locus_tag="VFR00_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate synthase subunit alpha"
                     /protein_id="HEU0157739.1"
                     /translation="MPALAERRPVKEQLAISNRDRATGAMLSGEIAKRYGHGGLPEDS
                     VWLSFTGTAGQSFGAWLAHGVTLDLVGEANDYVGKGLSGGKLIVRPDPASGITPEESI
                     IVGNTVLYGAISGECYFRGVAGERFAVRNSGAVAVVEGTGDHGCEYMTGGIVVVIGPT
                     GRNFAAGMSGGIAYVLDEDGTFERRCNLSMIDLEPVEAEDQVMRQLSNQGGDLESHGL
                     VDIMGDMTKQDAERLHQLIARHAHYTNSAKAKAILDNWAQYQGKFKKVMPVEYRRALE
                     EMSKHQAADKTGFDVLEIGLPVKG"
BASE COUNT          626 a         1250 c         1178 g          687 t
ORIGIN      
        1 tatcatgttt tgatcaaggg cgagcggctg cccacaaaac ccgggcgggc cgacgacttc
       61 ggctggccgc gcgtcgagcc cgacatcccc gaacccgggc cggtgcgacg ggcgcgaccg
      121 agcccgccca agcctgccgc accgccgcgc agctgaattc cagcgcccgc ggctgcggcg
      181 cacgaggctt gtgcacttgg cgcggcttgt gcgcttgacg cgctcacgcc gtagctggac
      241 cccggtcggt ggcatttggc cgacggcgac cgcgttccaa ccggctgcgc tgcccgcgct
      301 tgctcgcggt gccatcgacc ttgcgcaagg ccgcctcaaa ggcatcgcgc agcgccatcg
      361 tcagctcttc gctggcgtgg ctgccgaccc gttcatgaca gaccacgatg ggccgctggc
      421 ggggaactcc gatttcgatc ttgatacagt agactttcgg cttcagcgcc gtgcgttggg
      481 tggcctcgag cacgacgcgg cagcttgtca tgcgctcgaa atgcttctcc agtttggcaa
      541 ccttgtcgcg tacccgctgc tcaatggctt cgcttctctg gatcccgtgg aaattcacct
      601 cgaccggagc gttcatgggt aaaccccgtc tgctgagatc ggacacgacg tatctcagca
      661 cgtagggcgg gcgctggcca gatcaagagc gcggtttcac ttaagatttt ccgccccagc
      721 gccggcctag cgggcgagat cgctgcggcc catcaggaag ctgtcgatgg cgtgcgccgc
      781 ctggcggccc tcgcgaatgg cccacaccac caggctctgg ccgcgccgga cgtcgccggc
      841 ggcaaacagc ttgggcttgc ccttgatgcg gtaatcacgc tcgttggcgt caagcccctt
      901 gaagcgcccg cgcggcacca cgctgaggcc cagctcggcc accaggtcgc tttccaccgg
      961 gccggaaaag cccatggcga acagcaccag atcggccggc agctcaccct ccttgccggc
     1021 aatcggcttc agcgtggcat cgacgcgcgt atagagcagc ttttccacct tgccgttgcg
     1081 gccggcgaag cccgtggtgg agatcgagta ctcggtctgg gcaccctcgg cctggctgga
     1141 ggagacgcgc agcttcagcg gccagtgcgg ccaggacagc gccttgttct ccttgaccgg
     1201 gggctgcggc atgatctcca gctgcgtcac cgacttggcg ccctgacgaa acgaggtgcc
     1261 gatacagtcg ctgcctgtat cgccgccgcc gatgacgatc acgtgcttgc cttgcgccgt
     1321 gatctgcgcc tcgtcctgcg gcggctgacc ggcaacgcgc cggttctgct gcggcagaaa
     1381 ggtcatggcg aacaccaggc cgtcgagctc gcgccccggc gatttcgcaa aaaagtcgaa
     1441 cggttgttcc gagcctgccg ccagcagcaa ggcgtcgtgc tcgctctcga gccgttgcgc
     1501 cggcaggtcg cgtccgacat gaacgttgct gtggaagaca acgccttcgg cttccatctg
     1561 cttgatgcgg cgctcgacaa tgctcttttc catcttgaag tcgggaatgc cgtagcgcag
     1621 cagtccgccg ggggtgggat gcttgtcgta gacgtgtacc tcgtggccgg cgcgcgccag
     1681 ctgctgcgcg gccgccagac ctgccggccc ggaaccgacg atcgcaacct tcttgccgct
     1741 cttcttctcc gctggctcgg gcacaaccca gccctcggca aaagccttgt cggcgatact
     1801 gcactcgatg gtcttaatcg tcaccggcgt gtcgtcgatg ttgagggtac aggcggcctc
     1861 gcacggggcc ggacagacgc ggccggtaat ctcgggaaaa ttattggtcg agtgcaaatt
     1921 gcgcgcggcc gtgcgccatt gctcgcggta gacgagatca ttccagtccg gaatctgatt
     1981 gttcacggga caaccgttgt ggcagtaggg aataccacaa tccatgcagc gcgacgcctg
     2041 cacccgcgtg tccgcgtccg acagcggcac cacgaattcg cgccaatgct tcaggcgctc
     2101 ttcgaccgtg gcgtagccgc gatcgtgccg ctcgaactcc atgaagccgg tgattttacc
     2161 catcgtgtcg ccgctcttta attcaaggct cgagagggag cgtccgcagt gaggccgagt
     2221 gcaccctcgc agcgcgcaat ccaggcgcgc aggtctgggc gctggcaaag ctcaaagccg
     2281 ccctcggggg cgaggcgcgt atagggcagc aggacgagat cggcaatcga gaggttgtcg
     2341 ccgaccagcc accgccgccc cttgagcatg gcgtccatga aatcgagcgc cttttcccca
     2401 cgctcgacgc gctgcggctc gcgggcttcc ttcggccggc caagatagac catgtgaaag
     2461 cgacagaccg cgaggtaggg ctcgtggcta tactgctccc agaacaggag ctcgtcgacc
     2521 ttggcggcaa cgaacgggtc cgtcggcagc agcgggctgc cctgtgccag gtagcgcacg
     2581 atgccgttgg actgggccaa gcaccgacca tcatcgaatt ccacggccgg gatctgcgcc
     2641 attggaaact tggcgacgaa ctcgggcgta gttgtttccc cttttagcac ctcgaccggc
     2701 acccaggcgt aagcgagccc gagcaggtcg gccgtgtact tgaccttcag gcagttgccg
     2761 gaccccagat cgccgtagat cttcatgaca aaattcgctc gtgctgaaca atactgcgcc
     2821 ttgccgcggc cggcgcttag cctttcaccg gcaagccgat ctcgagcacg tcgaagccgg
     2881 tcttgtcggc ggcttggtgc ttgctcattt cttccagcgc tcgccggtac tcgaccggca
     2941 tcaccttttt gaatttgccc tgatactgcg cccaattgtc gagaatggcc ttcgccttgg
     3001 cggaattggt gtagtgggca tgccgggcaa tcagctggtg cagacgttcg gcatcctgct
     3061 tggtcatatc gcccatgatg tcgaccagac cgtggctttc gagatcgcca ccctggttgg
     3121 acagctggcg catgacctgg tcttcggcct ccaccggttc aaggtcgatc atcgacagat
     3181 tgcagcgccg ctcgaaagtg ccgtcctcgt ccagcacgta ggcgatgccg ccggacatgc
     3241 cggcggcaaa gttgcgaccg gtcggcccga tcacgacgac gatgccgccc gtcatgtact
     3301 cgcagccgtg atcgccggtg ccctccacca cggcgacggc gcccgagttg cgcacggcaa
     3361 agcgctcgcc ggcaacaccg cggaaatagc attctccgct gatggcgcca tagagcaccg
     3421 tattgccgac gatgatgctt tcctcggggg tgatgcccga ggcagggtcg ggacgcacaa
     3481 tcagcttgcc gcccgacagg cccttgccga cgtagtcgtt ggcctcgccg acgaggtcga
     3541 gggtcacgcc gtgcgcgagc caggccccaa agctctggcc cgccgtgccc gtaaaggaca
     3601 gccacacgct gtcctcgggc agaccgccgt ggccatagcg cttggcgatc tcgccggaca
     3661 acatcgcccc ggtggcccgg tcacggttgg aaatggcaag ctgctccttg acggggcgcc
     3721 tctcggctag ggccggcatg g
//
LOCUS       DASTMT010000018         3974 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_5254667, whole genome shotgun sequence.
ACCESSION   DASTMT010000018 DASTMT010000000
VERSION     DASTMT010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3974)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3974)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3974
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5254667"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..253
                     /locus_tag="VFR00_00430"
     CDS             <1..253
                     /locus_tag="VFR00_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003601172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3617 family protein"
                     /protein_id="HEU0157740.1"
                     /translation="PQQGLGALSENNPYSHCPVANLRQEGSTLSFDIVCPGGNAAVAS
                     ATYHLAREVFDGRISMKLGGKNMTLTETQSGRRIGDCQP"
     gene            complement(250..1179)
                     /locus_tag="VFR00_00435"
     CDS             complement(250..1179)
                     /locus_tag="VFR00_00435"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEU0157741.1"
                     /translation="MASQDYSERFRGAGPVGFLLIHGLGGTPVELRFVARGLARAGHT
                     VHCPQLAGHCGSFEDLKATGWKDWYATVRAAHQELSKSCKQVIVGGLSMGAILALHLA
                     AEFPADVHATTLFAPTLKLDGWGVPWYSIFFNLVRSEWSANFVRFAEREPYGIKDPRV
                     RALVTAAIQSGDSSKAGQLTNPGRVMLEMRRLVKVVRGELRRIHQPTLIVHPRQDDRA
                     SLGNAAYLQSALPGPVETCVLDDSYHVITIDRQRDIVIDRTAAFAKRLQDGSAAGDDL
                     KPRRDVRHSGSLPHVSRGRALGAASALLQGAAR"
     gene            complement(1226..1888)
                     /locus_tag="VFR00_00440"
     CDS             complement(1226..1888)
                     /locus_tag="VFR00_00440"
                     /inference="COORDINATES: protein motif:HMM:TIGR01488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family phosphatase"
                     /protein_id="HEU0157742.1"
                     /translation="MLGQVYVDFDGTIAPSDPTDRVFARFCGPKWLSLERACQQGRLS
                     PRATMAQQVELLRATPAALSAFVDELKIDPEFAAFVALCRRWDLRVVVVSDGLDRVVL
                     SMLRAAGFELPVFANRLTWLGGERWKLDFPYAASACAASLGNCKCAHRSPRALPSLAV
                     VVGDGRSDFCIAERADLVLAKGQLAAHRRRRNLPHQPIDNFAHATVALDGWLAQNALK
                     SA"
     gene            complement(2088..2282)
                     /locus_tag="VFR00_00445"
     CDS             complement(2088..2282)
                     /locus_tag="VFR00_00445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0157743.1"
                     /translation="MAKPKFTARIDNIYFLARIGSSLRWATQGVPPEALSSDVKHMLE
                     RLDQLDAVAQRLARPDNDRA"
     gene            complement(2509..3342)
                     /locus_tag="VFR00_00450"
     CDS             complement(2509..3342)
                     /locus_tag="VFR00_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HEU0157744.1"
                     /translation="MSVDFSTLIWILLGLMALQPLLMGRWFAFNRLKAIQALEKARGS
                     RVITMIHRQEKRSLFGFNVARHIDLEDAQTIIAAIKETPPERPIDLILHTPGGLVLAA
                     MQIARAVEAHAGKVTVFVPVYAMSGGTLIALAADEIVLGEFSVLGPIDPQIAGFPAAS
                     IVRVRQAKPIEHIFDLTLVLADMSEKALAQVKRGAVELLTPRLDQAAAEQLAEKLAGG
                     HWTHDYALTAQEASALGLPVTVAMPVQVLELMKLYPQPVQRSGVEFLPIDVPAKRAMG
                     T"
     gene            3526..3849
                     /locus_tag="VFR00_00455"
     CDS             3526..3849
                     /locus_tag="VFR00_00455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0157745.1"
                     /translation="MQNAYVASDLLPAMAFWLVAVLAAAPAFGIVRQSKRGRARSAIA
                     YLTGFFVGLAASVGLALAAAALLSTESPIFNAGVTAAFLGPFTGIVHAKLRRNARRRS
                     KWAEA"
BASE COUNT          664 a         1313 c         1333 g          664 t
ORIGIN      
        1 gccgcagcag gggctcgggg cgctcagcga gaacaatccc tattctcact gtcccgttgc
       61 caatctgcgc caggagggaa gcacgctcag cttcgacatc gtttgccccg gcggcaatgc
      121 cgccgttgcc tcggccacct accatctcgc gcgggaggtt ttcgacggcc gcatcagcat
      181 gaagttgggc ggcaagaaca tgacgctgac ggaaacccag agcggccgcc ggatcgggga
      241 ttgtcagccc tagcgtgccg cgccttgcaa aagcgcgctt gcggcgccca gggcacggcc
      301 gcggctgacg tgcggcaggg accccgagtg ccggacatcg cgccttggct tcagatcgtc
      361 gccggcggcg gacccatcct gcaggcgctt ggcgaaggcc gccgtgcgat cgatgacaat
      421 gtcgcgctga cggtcgatcg tgatgacgtg atagctatca tccagcacgc aggtttccac
      481 tggtcccggc agcgcgcttt gcagataggc ggcattgccg aggctggccc gatcgtcctg
      541 gcgcgggtgc acgatcaagg tgggttgatg gatgcgccgc agttcgccgc gcacgacttt
      601 caccaggcga cgcatctcca gcatgacgcg gccggggttc gtcaattgcc cggccttgga
      661 gctgtcgcca ctttggatcg cggcggtgac gagggcacgc acgcgggggt ccttgatgcc
      721 atagggctcg cgttccgcga agcgcacgaa attggcgctc cactccgagc gcaccagatt
      781 gaaaaagatg ctgtaccagg gcacgcccca accatcgagc ttcagggtcg gtgcaaacag
      841 cgtggttgcg tgcacgtcgg ccggaaactc ggcggccagg tgcagcgcca aaatcgcccc
      901 catggacagg ccgccgacga tcacctgttt gcagctcttg ctgagctctt ggtgcgccgc
      961 ccgcacggtc gcataccaat ccttccaacc ggtcgccttc agatcctcga agctgccgca
     1021 atggccggcg agctgcgggc agtgcacggt gtgccccgcg cgcgccaggc cgcgggcgac
     1081 gaagcgcaac tcgaccggcg tgccgccgag gccatggatg agcaggaagc ccacggggcc
     1141 cgcgccccga aatcgttcac tgtagtcttg gctggccatc agggtcctgt tcgctggagg
     1201 gatgtgcgat cgagcgcggc ggggctcacg cggacttcag cgcgttctga gccagccagc
     1261 cgtcgagcgc cacggtggcg tgggcaaaat tgtcgatggg ctgatgcggt aggttgcgcc
     1321 gccggcggtg tgccgccagc tgcccttttg ccaacaccag gtcggcccgc tccgcgatgc
     1381 aaaaatccga gcgcccgtcg ccgaccacaa cggccagcga gggcagcgcg cgcggcgaac
     1441 ggtgggcaca tttgcagttg ccgagcgagg cggcgcacgc gctcgcggcg tagggaaagt
     1501 cgagcttcca gcgctcgccc ccgagccagg tcaagcggtt ggcgaagacg ggcagctcaa
     1561 agcccgcggc ccgcagcatg ctgaggacga cgcgatcaag gccgtccgaa accacgacca
     1621 cgcgcaaatc ccagcgccga cacaaggcca cgaaggcggc aaactccggg tcgatcttga
     1681 gctcgtcgac gaaggcgctg agcgcggccg gcgtggcccg caagagctcg acctgttggg
     1741 ccatggtggc gcgggggctc agccgccctt gctggcaggc gcgttcgagg ctcagccact
     1801 ttggcccgca aaagcgcgcg aacacccgat cggtcggatc actcggcgca atcgtgccat
     1861 cgaagtcgac atagacctgt cccagcattg catccccttc aattcgccac gcctccccaa
     1921 gtttgttttt gtgcagtgca aacgccttat ggcccctcga cctgaggcaa aaaagtgtca
     1981 aaccctcccg gccgttatca cagtattttg acttggcgcc ttaacccctg cgttgccgct
     2041 cgacctgcgg caataggcgc aggcgttggc gcgccccgcg cggcggctca ggcgcgatcg
     2101 ttgtcgggcc gcgccagcct ttgcgccacg gcgtcgagct gatcgagccg ctccagcatg
     2161 tgcttgacgt cgctcgaaag cgcctcgggc ggcacgccct gcgtggccca gcgcagcgag
     2221 gagccgatgc gcgccagaaa gtagatattg tcgatgcgcg cggtaaactt tggttttgcc
     2281 atgctcgagc gggaacgatg aggttttgct ttggcgcggg cgccgtcgcg cccacgcgag
     2341 aggtggcggc gtggccgagg gtcacctcgg cggcaggccg ctgctctgcg cctcagatgc
     2401 gccttgcaac gattttccac atccccgctc taggggagat taggcgaggg cgcccccgta
     2461 acgcggcagg cgccgccccg atcgtccttt ttgtcgcagg ttgccgcctc aggtccccat
     2521 cgcccgtttg gccggcacgt cgatcggcag gaattccacg cccgagcgct gcacgggctg
     2581 cgggtagagc ttcatcagtt ccagaacctg aaccggcatg gcgacggtga ccggcaggcc
     2641 caaggcggag gcttcctgcg cggtcagcgc atagtcgtgc gtccagtgcc cgcccgccag
     2701 tttttcggcg agctgctcgg cggcggcttg atccaggcgc ggggtcaaga gttccaccgc
     2761 gccgcgcttg acctgggcca gggctttttc gctcatgtcg gccaagacaa gggtcaaatc
     2821 gaaaatgtgc tcgatgggct tggcttggcg cacgcgcacg atgctggccg cgggaaaacc
     2881 ggcgatttgc gggtcgatcg ggcccaacac cgagaactcg ccgagcacga tctcgtcggc
     2941 cgccagcgca atcagggtgc cgccggacat ggcgtagacc ggcacgaaca ccgtgacctt
     3001 gcccgcgtgc gcctcgacgg cgcgggcgat ttgcatggcc gccaacacca ggccgccggg
     3061 cgtgtgcaaa atgagatcga tcgggcgttc cggcggcgtc tccttgatgg ccgcgatgat
     3121 ggtttgggca tcttcgaggt cgatgtggcg agccacattg aagccaaaca ggctgcgctt
     3181 ttcctgccga tggatcatgg tgatgacccg gctgccgcgc gccttctcca aggcctggat
     3241 ggccttgaga cggttgaagg cgaaccatcg gcccatcagc aagggctgca gggccatgag
     3301 acctagcaag atccagatca gcgtcgaaaa atcgactgac atgggtccgc tcccaagcgt
     3361 gttgccgagc caaggcgggt cacaatccga cccttgcgta accgatggca accgccccga
     3421 gcaacgtgat tttgcacgcc gccaagcgcc tgctgcggcg ctcggccaca ttttgtgcgc
     3481 gcgaatcacc taagccccag cgcccccaca gggcaggtga agacgatgca gaacgcgtat
     3541 gtggcgtcgg atttgttgcc ggcgatggcc ttctggctgg ttgccgtgct ggcggccgcc
     3601 cccgccttcg gcattgtgcg gcaaagcaag cgcggacggg cgcgcagcgc cattgcctat
     3661 ctgaccggct ttttcgtcgg cctggcggcg agcgtgggcc tggcgctggc ggcggccgcc
     3721 ctgctcagca cggaaagccc catctttaac gccggggtga cggccgcctt tttgggcccc
     3781 tttaccggca tcgtgcacgc caagctgcgc cgcaacgcgc ggcggcggag caaatgggcc
     3841 gaggcctaag cgctcgtgcc ggtcccgtcg cgcaaagccg ttcccagcgc gcgggctctc
     3901 cattaggctg cgcttcccgc cggcggccgc gtgcgcggtg cgggacctgg cgaggaggct
     3961 tggcgtgctg cgga
//
LOCUS       DASTMT010000019         2556 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_2305149, whole genome shotgun sequence.
ACCESSION   DASTMT010000019 DASTMT010000000
VERSION     DASTMT010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2556)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2556)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2556
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2305149"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..473
                     /locus_tag="VFR00_00460"
     CDS             <1..473
                     /locus_tag="VFR00_00460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0157746.1"
                     /translation="DKRYAAIPLANAANVDATVAAAVLVWPVIDPLRRYHHAKRLQAA
                     GGTYPEQIDRVIPLHLKFWQNEEAMEEGNPVGILERGEAVELPPVLYVQGGKDVVHPR
                     ADLERFVASYRRRGGVVDLALYEGEAEGFIRNAASAAAPQAIARIIDFVHKHLA"
     gene            619..1182
                     /locus_tag="VFR00_00465"
     CDS             619..1182
                     /locus_tag="VFR00_00465"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007790826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEU0157747.1"
                     /translation="MQNAEQHYVIFETAGGFCGIAWNQRGITRFQLPVKTAEAASRHL
                     LRRVPDATPGTPTPDARAAIAAAKSYFAGAQTDFAEFKLDLGEPGAFFEQVYAAARRI
                     GWGHTTTYGALAKELGAGSEAARDVGQAMAQNPVPLIIPCHRVLAAGGKLGGFSAPGG
                     ASTKRRMLALEGIALAPPPSAQGAFEF"
     gene            complement(1237..1494)
                     /locus_tag="VFR00_00470"
     CDS             complement(1237..1494)
                     /locus_tag="VFR00_00470"
                     /inference="COORDINATES: protein motif:HMM:NF013043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HEU0157748.1"
                     /translation="MPRFVYPNGSGFRALIRNGDKILYLGTFDTPEKASEAAERMAKE
                     LRGKADARKTDARPKRPRPIAAKVKARRRRVTSKRRRARSS"
     gene            complement(1880..>2556)
                     /locus_tag="VFR00_00475"
     CDS             complement(1880..>2556)
                     /locus_tag="VFR00_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013948521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2778 domain-containing protein"
                     /protein_id="HEU0157749.1"
                     /translation="AEKKVLPQWDCAPGIDFVTIQHKGYERKLTDGEICSPFDDVVRQ
                     VPEQLKSFRLGYTIRTGRLFVLADDPQLGGKTIAWAISGREACRNNPDPDCLAARALG
                     PLPPGVYSFTSDKEGRVSWGPKTKRMVAAIYLNKLWYREKFTPAQTAAILARGNIAIH
                     VRLKGEMSEACLGLGPNGWGYVASLIKDGRATGLNVYIDEPYPQIAETPPIVRVSSFS
                     LSSLFK"
BASE COUNT          449 a          813 c          818 g          476 t
ORIGIN      
        1 gagataagcg ctacgccgcc attcccctgg cgaacgcagc caatgtcgat gccacggtcg
       61 ccgcggctgt cctggtttgg ccggtgatcg atccgctgcg ccgttatcat cacgccaaac
      121 gcctgcaggc cgcgggcggc acctaccctg agcaaattga tcgcgtcatc cctctccacc
      181 tcaagttctg gcagaacgag gaagcgatgg aggagggcaa tccggtcgga attttagagc
      241 ggggcgaagc cgtcgagctg ccacccgtgc tctacgtgca aggcggcaag gacgtcgtgc
      301 acccaagagc cgaccttgaa cgcttcgtgg cgagctaccg gcgccgcggc ggggtggtcg
      361 atctcgccct ctatgaaggg gaagccgaag gcttcattcg caacgccgct tctgcggcgg
      421 ctcctcaggc gatcgcacgc attatcgatt ttgtgcataa acatctggcg tgatcgtcag
      481 gcccctggct gggcctccgc cgatgctggc tggcccaggc actcggcggg tgggctcgcc
      541 aaagcgcagg tatcgggttg aagtctcttg ctggcggcac aatgtgctgc aaaacccgcg
      601 gagacattgg gtgcagccat gcaaaatgcg gagcaacact acgtcatctt cgagaccgcc
      661 ggtggctttt gcggcatcgc ctggaaccag cgggggatca cacgcttcca gctgccggta
      721 aaaacggccg aggcggccag ccgacatctg ctgcgccgcg tgccggacgc cacgcccggt
      781 acgcccacgc cggatgcgcg cgcggccatt gccgcagcga aaagctactt tgcgggggcc
      841 caaacggatt ttgccgagtt caagctcgac ctcggtgagc cgggggcctt tttcgagcag
      901 gtatacgcgg cggcccggcg catcggctgg ggccatacca ccacctatgg cgctttggcc
      961 aaggaactcg gcgctgggtc tgaggccgcg cgcgatgtcg gccaggccat ggcgcagaat
     1021 ccggtgcctt tgattattcc ctgccaccgc gtcctggccg cgggcggcaa gcttggaggc
     1081 ttttcggcgc ccggcggcgc cagtaccaag cggcgcatgc tcgcgctgga gggcatcgcg
     1141 ctcgccccgc caccatcggc gcaaggcgcc ttcgagtttt aggcgatcgg cgaaaccatc
     1201 ctcggcccag ccgcgcaatc ggagtggctt cggatctcag ctggatcgcg cacgccgtcg
     1261 tttcgacgtg acccggcggc gccgggcttt taccttggcg gcgatcgggc gaggccgttt
     1321 cgggcgggca tctgtcttgc gggcatccgc cttgccgcgc agttccttgg ccatgcgctc
     1381 ggcggcctcg cttgcctttt caggcgtgtc gaaggttcca agataaagaa tcttgtcgcc
     1441 attgcgaatg agagcccgaa acccgctccc gttggggtag acgaagcgcg gcaaatccgg
     1501 attgatggtg cgccggcgac ttggctgccg ggctgccgtt cgcatgactg atcctcagca
     1561 acgtctgcct agctgggttc gccagcccgc aatcccattg gcgaatgcca gcataaaacc
     1621 tggagttgct acagctgcaa gcctgcaggc gagaacacgc tgctttctgg ctcggagcgt
     1681 gacccgcgag ttgcgggcct tccccacggc cgctttggca tcggaccccg ctcgcccacg
     1741 cgcatcaatc gtttgcccag gcgcgggggg cgaagaccgc gcgccgttga taagttggct
     1801 tgcaggcgac gcgccgacac ccagggctga tgggcgccct ggcgctgtgt ccgtctcgcc
     1861 gccggcgcag ggcaaagaac tacttaaaga gcgatgacag tgagaacgag gaaaccctga
     1921 caatcggggg tgtttcggca atctggggat agggttcgtc gatatagacg tttaatcccg
     1981 tggcgcgacc atctttgatg agcgacgcga catagcccca gccatttggc cccagcccca
     2041 gacaggcttc ggacatttcg cctttgaggc gcacgtgaat ggcgatattg ccccgcgcca
     2101 ggatggccgc ggtctgcgcc ggcgtaaact tttctcgata ccagagcttg ttcaagtaga
     2161 tggccgcgac catgcgcttg gtcttgggcc cccagctgac gcggccttcc ttatcggacg
     2221 tgaaagagta gacacccggc ggcagcggtc ccaaggcgcg cgccgccaaa cagtccgggt
     2281 cagggttatt cctgcaagcc tcccgaccag aaatggccca ggcgatcgtt ttaccgccaa
     2341 gctgtggatc atcggcgagg acaaacaggc gaccggtcct gatcgtgtag ccaagtcgga
     2401 agctcttcag ctgctcgggg acctggcgca cgacgtcgtc gaacgggctg caaatttccc
     2461 cgtccgtcag ttttctctca tagcccttgt gttggatggt gacgaagtcg atgccgggag
     2521 cgcaatccca ctgcggcagc actttcttct cggcaa
//
LOCUS       DASTMT010000020         3433 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_3946594, whole genome shotgun sequence.
ACCESSION   DASTMT010000020 DASTMT010000000
VERSION     DASTMT010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3433)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3433)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3433
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3946594"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..566)
                     /locus_tag="VFR00_00480"
     CDS             complement(<1..566)
                     /locus_tag="VFR00_00480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0157750.1"
                     /translation="MEGFDVKAALDALVAFLGQNYLPLLLGLVALILFRRSGVFARVR
                     GALEKALIDNWQLTLLASTGVALSLASAYTTWDGLRNFTRAPLLSLAIAFGIQGVMLI
                     VAWLIGESFAAGMNQRAPDGRRARLPDALIGMALGVTLVGLLFYWVLQHYDAITLSSA
                     AGFRADLAKVGEIGLYFLIGLVLIAIVA"
     gene            complement(834..1370)
                     /locus_tag="VFR00_00485"
     CDS             complement(834..1370)
                     /locus_tag="VFR00_00485"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006609189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HEU0157751.1"
                     /translation="MPHRLEIIEADITTLDVDAIVNAANRTLLGGGGVDGAIHRAAGP
                     ELLAECRTLGGCATGEAKITRGYRLKARAVIHTVGPIWQGGAAGEAVLLANAYANSLK
                     LARGHGLASLAFPAISTGAYGFPADLAADIALATVLAALTGDQHFRRVIFCCFGAASR
                     AQHERARDKVLTRAGSPP"
     gene            1505..2023
                     /locus_tag="VFR00_00490"
     CDS             1505..2023
                     /locus_tag="VFR00_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020083988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEU0157752.1"
                     /translation="MTQVLPSSAFPAPEHDHGHCLAEAMERARKAFEDKGLKLTGLRR
                     SVFAEIAASHKAIGAYEVLARLGARGERLAPISVYRAIDALVAVGIVHRFESRNAFFA
                     CHVGHPARRQLVLACEACRSVAEIDGDAVFRAIDKAADAAAFSAKEAVVEVWGLCANC
                     AGAAREPGHGRA"
     gene            2010..2918
                     /locus_tag="VFR00_00495"
     CDS             2010..2918
                     /locus_tag="VFR00_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEU0157753.1"
                     /translation="MDGRERQSQPRPRAAGQHAARPPERPWNGEAVSQAPAPGNRLER
                     RAEPELISARAILLSRGGRTLLQDVDIDVASGEIVTLIGPNGAGKTTLVRVLLGILLP
                     NRGEVRRKAELRIGYVPQRLEVDRSIPLTVGRFVALGRRSAGTQIEAVLAEVGAQALL
                     ERQLNELSGGELQRVVLARALVRSPELLVLDEPVRGVDYAGEAELYTLIGRLRSERRL
                     GVLLVSHDLHIVMAQSDRVICLNHHVCCSGVPHSVAQHPEYARLFGPQASRAIGLYQH
                     HHDHSHDLTGAPQPPVSPRPGPQEFK"
     gene            2915..>3433
                     /locus_tag="VFR00_00500"
     CDS             2915..>3433
                     /locus_tag="VFR00_00500"
                     /inference="COORDINATES: protein motif:HMM:NF013143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEU0157754.1"
                     /translation="MTEPFLMRALLGGMGLALVAAPLGCFVVWRRMAYFGEAVAQSGL
                     IGIAIGLALALNLTASVLVVTLIVSGLLVLLGRQNLVPIDTLLGLIAHAALALGVIIA
                     SLVQGPQLDLMTFLFGDIFAISRADLRWIYFGGVVTLAVEIWLWRPLLAIAVDEDLAA
                     AEGVRTQYFKAAF"
BASE COUNT          554 a         1125 c         1171 g          583 t
ORIGIN      
        1 aaggcaacga tggcgatcag caccaatccg atcaggaagt agagaccgat ctcgccgacc
       61 ttggcgagat cggcgcgaaa gcccgcagcg ctgctgagcg tgatggcgtc gtaatgctgc
      121 aggacccagt agaagaggag acccaccagc gtcacgccga gcgccatgcc gatgagggcg
      181 tccggcaggc gggcgcgccg cccgtcggga gcgcgctggt tcatgccagc agcgaaactt
      241 tccccgatca accaggccac gatcagcatg acgccctgaa tgccgaaggc aatcgccagc
      301 gacaacaggg gcgcgcgggt gaagtttcgc aaaccatccc aggtggtgta ggcggaggcc
      361 agcgacaggg ccacgccggt ggaggccagc agcgttagct gccaattgtc gatcagggct
      421 ttctcgagag cgccgcgcac gcgggcgaac acgccgctgc ggcggaagag gatgagagcc
      481 acgagcccga gcagaagcgg caggtaattt tgaccgagga acgccaccag cgcatcgagc
      541 gccgccttga cgtcgaagcc ctccatcctg tcgtctcccg ctacccgagc ttgacctgtt
      601 gcgccgcttc cagcatcgcc cgctaaggcg gcggcagggc ggctgctttg cggcgatatt
      661 gttgcctgcg cagtgaaatg caatgcggct gccgggccgc tgcgcgggca ggggaggcgg
      721 gcaaagatcg cacgccgccg caacacgttt gggcaagctg ttgcttgctc atgaataagc
      781 gcgctgcggc gcgccgggat cgcggcgcga agcgcggtcg cattaaagat tcgttaagga
      841 ggactgcccg cgcgcgtcag caccttgtcc cgcgcgcgct cgtgctgggc gcgcgaggcg
      901 gcgccgaagc agcaaaagat cacgcgcctg aaatgctgat ccccggtgag ggcggcgagc
      961 acggttgcga gcgcgatgtc ggcggcgaga tcggccggga agccgtaggc tccggtcgag
     1021 atggcgggaa aggccaacga ggcgaggccg tgcccgcggg caagtttgag cgagttggca
     1081 taggcatttg ccaacagaac agcctcgcca gccgcgccgc cctgccagat cggaccgacg
     1141 gtatgaatga cggcgcgcgc cttcaggcga tagccgcgcg tgattttggc ttcccccgtg
     1201 gcgcagccac caagcgtgcg gcattccgcc aaaagctccg gcccggcggc acgatgaatg
     1261 gccccgtcca cgccaccgcc acccaacagc gtgcggttgg ccgcgttgac gatggcatcc
     1321 acgtcaagcg tggtgatgtc ggcctctatg atttccaggc gatgcggcat cgacgtcctc
     1381 gccgtgtgcc gccggctggc cacggcggca aggccgctat ttagcgcatg tcgccgcccc
     1441 ggaatatacg atctttcccc tgcgtgtgga tggcacggcc tcggccgggt gctataaaga
     1501 gccgatgacg caggtcctcc ccagcagtgc ctttcccgca cccgaacatg accacggcca
     1561 ttgtctcgcc gaggccatgg agcgtgcgcg caaggccttc gaggacaagg gtctcaaact
     1621 gaccgggctg cgccgctcgg tgttcgctga aattgccgcc tcgcacaagg ccatcggtgc
     1681 ctacgaggtc cttgccaggc tgggcgcgcg cggcgagcgg ctggcgccga tttcagtgta
     1741 tcgggcgatc gatgccttgg tggcggtcgg catcgtccac cgcttcgaaa gtcggaacgc
     1801 cttttttgcc tgtcacgtcg gccatcccgc gcgccggcag ttggtgctcg cctgtgaggc
     1861 gtgccgatcg gtcgcggaaa tcgacggcga tgccgtgttc cgcgcgatcg acaaagccgc
     1921 cgacgcggcg gccttttcag ccaaggaggc ggtggtggaa gtttggggcc tgtgcgccaa
     1981 ttgtgccggg gcggcacggg agccgggcca tggacgggcg tgagcgccaa tcccagccgc
     2041 gaccgcgcgc ggccggccag catgccgccc ggccccccga gcggccgtgg aacggcgagg
     2101 cggtgagcca agcacccgcg cccggcaacc ggctcgagcg acgcgcggag cccgagctca
     2161 tttcggcacg ggctatcctc ttgagccgtg gcgggcgcac gctgctgcag gacgtggaca
     2221 tcgacgtggc ttccggcgaa atcgtcacgc tgattggtcc caatggtgcg ggcaagacga
     2281 cactggtgcg cgtgctgctc ggcatccttt tgcccaaccg gggtgaggtg cggcgcaagg
     2341 ccgagctgcg gatcggctac gtgccccagc gcctcgaggt cgatcgcagc attccgctga
     2401 cggtcggccg cttcgtggcg ctcggtcgcc gcagcgccgg tacgcaaatc gaggcggtgc
     2461 tggccgaggt gggagcgcaa gccttgctgg agcggcaact caacgagctc tccggcggcg
     2521 aactgcagcg cgtggtgctc gcgcgcgccc tcgtgcgctc acccgagctg ttggtgctcg
     2581 acgagccggt gcgcggcgtc gactacgccg gcgaagccga actctatacc ctgatcggcc
     2641 ggcttcgctc cgagcgcagg cttggcgtgc tgctggtctc gcacgacttg catatcgtta
     2701 tggcgcaaag cgaccgcgtc atttgcctca atcatcacgt ctgttgttcg ggcgtgccgc
     2761 attcggtcgc acagcacccc gaatacgccc gcttgttcgg cccgcaggcc tcacgcgcga
     2821 tcgggcttta ccagcaccat catgaccaca gtcatgatct caccggcgca ccccaaccgc
     2881 cggtttcgcc aagacctggc ccgcaggagt tcaagtgact gaaccctttt tgatgcgggc
     2941 cctgctcggc ggcatgggtc tggcgctggt tgctgccccg ctcggttgct tcgtggtgtg
     3001 gcgccgaatg gcgtacttcg gcgaggcggt ggcccagtcc ggcctgatcg ggattgccat
     3061 cggcttggcg ctggcgctca acctcaccgc cagcgttctc gtcgtgacgc tcatcgtttc
     3121 gggcctgctg gtcttgctgg gtcggcaaaa cctggtgccc atcgatacgc tgctgggtct
     3181 gatcgcccat gcggccctgg cgctcggcgt catcatcgcc tccctggtgc aggggccgca
     3241 gctcgacctc atgaccttcc tgttcggcga catctttgcc atcagccgcg ccgacctgcg
     3301 ctggatctat tttggcggtg tcgtgacgtt ggcggtcgag atctggctct ggcgtccgct
     3361 gttggccatt gcggtcgacg aggacctggc cgcggcggaa ggcgtgcgca cccaatactt
     3421 caaggctgca ttc
//
LOCUS       DASTMT010000021         1812 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_2633111, whole genome shotgun sequence.
ACCESSION   DASTMT010000021 DASTMT010000000
VERSION     DASTMT010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 1812)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1812)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1812
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2633111"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..262
                     /locus_tag="VFR00_00505"
     CDS             <1..262
                     /locus_tag="VFR00_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008936244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEU0157755.1"
                     /translation="LAQRIVAELKDKAPGLAVAALGAPGGKKGAAAPPAGDAAAEAIS
                     ALTNLGYQPAQASQAVAVALNELGEVVDTAKLIRRGLKELAR"
     gene            362..1279
                     /locus_tag="VFR00_00510"
     CDS             362..1279
                     /locus_tag="VFR00_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006727931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEU0157756.1"
                     /translation="MQIYLPVAELSLNAYMLLGMGLAIGVLSGMFGIGGGFILTPFLI
                     FLGVPPGIAVGTGASQVVAASVSSALGHWQRGNVDLHMGLLLVAGGFLGAFAGVKVLY
                     WLRIWGQLDLMVALTYVILLGVVGALMLIESVRALRASPAKLATASHRGGQHGWIERL
                     PLKQRFRRSKRYISTIPLFVMGAFIGWLTAIMGVGGGFLLVPAMIYLLRIPTRIVIGT
                     STFQIVCVTALTTVLQAVQNHSVDVVLALPLIAGAVIGAQFGVGFGERLKAEQLRAGL
                     ALLVLAVAVRMALSLTVAPQNIYSMEPPV"
     gene            1580..>1812
                     /locus_tag="VFR00_00515"
     CDS             1580..>1812
                     /locus_tag="VFR00_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004445743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU0157757.1"
                     /translation="MSDADRLVTGAKRETDDAEPSLRPQYLREFIGQEQARANLKVFI
                     EAAKARGDALDHVLFAGPPGLGKTTLAQIMARE"
BASE COUNT          279 a          586 c          610 g          337 t
ORIGIN      
        1 gctggcgcag cgcatcgtgg cagagctcaa ggacaaggcg ccggggctgg cggtggccgc
       61 gctcggggcg cccgggggca aaaagggcgc cgccgcaccc ccggccggcg acgccgcggc
      121 ggaggcgatc tcggcgctta ccaacctcgg ctaccagccg gcgcaagcaa gccaggcggt
      181 ggccgtcgct ctcaacgagc tgggcgaggt ggtcgatacg gcaaaactca ttcgccgtgg
      241 gctgaaggag ctggcgcgat aggctatggt gccgcagcat tgcggcaatg cggcttcgga
      301 atgccttggc ggagccgcat tgacatccta gccgagccgg gctctcgtct tttgaaggcc
      361 gatgcagatc tatctccccg tcgccgagct ttcgctcaac gcgtacatgc tgctcggcat
      421 ggggctagcg atcggcgtgc tttcgggcat gttcggcatt ggcgggggat ttatcctgac
      481 gccgttcctg atttttctcg gcgtccctcc cgggattgcg gtcggtaccg gcgcgagcca
      541 agtggtggcc gcctcggtct ccagcgcgct cggtcactgg cagcgcggca atgtcgactt
      601 gcacatgggg ctcttgctcg tcgccggcgg cttcctcggt gccttcgccg gggtcaaggt
      661 cctctactgg ctgcgcatct ggggtcagct cgacctgatg gtggcgctca cctacgttat
      721 tctgctgggc gtcgtcggcg ccttgatgct gatcgaaagt gtgcgggcct tgcgcgcttc
      781 gcccgcgaaa cttgccaccg cctcgcatcg cggtggtcag cacggctgga tcgagcgcct
      841 gccgctgaag cagcgcttcc gccggtccaa gcgctatatc agcaccatac cgctgttcgt
      901 catgggagct tttatcggct ggctgacggc gattatgggt gtcggcggcg gcttcctgtt
      961 ggtgccggcc atgatctatc tgttgcgcat tccaacgcgc attgtcattg gtacctcgac
     1021 cttccaaatc gtctgcgtca ccgcgctgac gaccgtgctg caggccgtgc aaaatcacag
     1081 cgtcgacgtg gtgctcgccc tgcccttgat cgccggggcc gtgatcgggg cgcagttcgg
     1141 cgtcggtttc ggcgagcggc tcaaagccga gcagttgcgc gccggcctcg ccctcctggt
     1201 attggcggtt gcggtgcgca tggcgctcag cctgaccgtg gcgccgcaga atatctactc
     1261 catggagccg cccgtctaag ctcgcgagcg cgtggcgcag ccgcagccat gcaacggggc
     1321 cagtggaaaa gcggggagtg atcggggggc ttacccgtgg gaccgcggca gggaggagga
     1381 aaaaccgcgc cgctatcact ccccaggcta tcaattaggc aaaatgggcc cgcccgctgt
     1441 gctctggagc acgcgtggcc ccaggcgggc tgtgccatcg gcccacgcgg cccaaagcgg
     1501 ctggcacgcc gggaattgct cgcgcgcggc acatcgtcgt tcttgacgcc tcgctcgaat
     1561 agatccatca gaacaaacca tgtccgatgc cgaccgcctt gtgacggggg ccaagcgcga
     1621 gaccgatgac gcggagccct ctctgcgccc acagtacctg cgggagttca tcggtcagga
     1681 gcaggcacgc gccaacctca aggtgttcat cgaagcggcc aaggcccgcg gcgatgcgct
     1741 cgatcacgtc ttgtttgccg ggccgcccgg gctcggcaag accacacttg cccagatcat
     1801 ggccagggaa tt
//
LOCUS       DASTMT010000022         2913 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_1321495, whole genome shotgun sequence.
ACCESSION   DASTMT010000022 DASTMT010000000
VERSION     DASTMT010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2913)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2913)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2913
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1321495"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..844
                     /locus_tag="VFR00_00520"
     CDS             <1..844
                     /locus_tag="VFR00_00520"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU0157758.1"
                     /translation="DAEVVLGGWPFPRDLRARAPKLKWFHQRPAGASNLMLGDLWGSD
                     VLTTTSRGAGNTLAMAEYALAGILYFAKGLQRAAIDRDAGAFNHRAYRPLLLEGKTAL
                     IVGAGGIGLEVGRLCAALGMRVVGTRRRPATTPLPPGFSSIGGPDDLDRFLAESDVVA
                     ICCQWTPETDRLFNSERFARMKAGSVLVNVARGEIIDEDALAEALAAERLRGVVLDVY
                     TGEFEHTPMARLWSDPRVMITPHISNVSDQDRHGAIDVFCANLRAYLDGRPLRNAIDW
                     QRGY"
     gene            complement(873..1625)
                     /locus_tag="VFR00_00525"
     CDS             complement(873..1625)
                     /locus_tag="VFR00_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015933944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU0157759.1"
                     /translation="MQAFDSDGICIRFIDEGNGDPVLLVHGFASSVRYNWLDPGWVSL
                     LRRNGYRVIAYDNRGHGESEKLYEPQAYQPSVMMEDARRLLDHLGIARADVMGYSMGA
                     RLTAFLALAHPQRVRSAIFGGLGGNMTRPMAGTGPIAHALEVGSIEEVVNPTARTFRA
                     FADKTGSDLAALAACIRGAREPISREMVAKIACPVLVASGTEDVIGGSAEDLAQLIPG
                     AQALPIPRRDHMLAVGDRVYKEGVLAFLARRP"
     gene            1732..1983
                     /locus_tag="VFR00_00530"
     CDS             1732..1983
                     /locus_tag="VFR00_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009463639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HEU0157760.1"
                     /translation="MAVSIIPHLANDPGVEKIYIGVREFQCMGARPPHDHPHVYLDMG
                     ADPQILCPYCSTLYIHDARLGADQSDPPGCLVNEDQPRP"
     gene            2005..>2913
                     /locus_tag="VFR00_00535"
     CDS             2005..>2913
                     /locus_tag="VFR00_00535"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEU0157761.1"
                     /translation="MGNARPILVLGGGIAGLALALSLARHGRATVVLESRARFETEGA
                     GIQLGANGVKVLARLGLAERLRSEVGEPASLVVFDGRSARRLAQLPLGSWFAARHGAP
                     YWTMHRADLHAALTATAAQQPHLLLRANYALKAVRQETSGVLAEDRGGERLAGEALIG
                     ADGLWSAVRQTICPAARPQFAGATAARALIAASAAGGLAEPHVGLWLGTGANVVHYPV
                     RAGREVAVVIIVREPWLASTAESRVDASQALAPLAAFHHSLTDVLGRAVDYRKWALYR
                     LAALPAWSSERIGLIGDAAHPMLPHLA"
BASE COUNT          443 a          991 c          987 g          492 t
ORIGIN      
        1 cgacgcggag gtggttctgg gcggatggcc gtttccgcgg gacctgcgcg cccgcgcccc
       61 gaagctcaaa tggtttcacc agcgacccgc cggcgccagc aacctgatgc tgggcgattt
      121 gtggggcagc gacgtgctga cgacgacgtc gcgcggggcc ggtaacacct tggccatggc
      181 cgagtatgcg ctggcgggca tcctgtattt tgccaagggg ctgcagcggg cagcgatcga
      241 tcgcgacgcc ggcgccttca accatcgcgc ctatcgcccg ttgctgcttg agggcaaaac
      301 ggcgttgatc gtcggcgccg gcggcatcgg gctcgaggtc ggcaggctct gcgccgccct
      361 cgggatgcgc gtcgtcggga cgcgccggcg gcccgccacc acacccttgc caccgggctt
      421 cagcagcatc ggtgggcccg atgaccttga tcgtttcttg gccgaaagcg atgtggtggc
      481 catttgttgt caatggacgc ccgagacgga ccgcctgttc aacagcgagc gcttcgcgcg
      541 catgaaggct ggcagcgtgt tggtcaatgt cgcgcgcggt gagatcatcg atgaggacgc
      601 cctcgccgag gccttggcgg ccgagcggtt gcgtggcgtc gtgcttgacg tctacacggg
      661 cgagttcgag cacacgccca tggcgcggct ttggtctgac ccccgggtca tgatcacacc
      721 acatatctcc aacgtcagcg accaggatcg gcacggcgcc attgacgtgt tttgtgcaaa
      781 cctgcgcgcc tacctcgacg gccggccgtt gcgcaatgcc attgattggc agcgcggcta
      841 ctagcccgaa gtagccaggg gcgcggcggc agctacggcc gtctggccag gaaggccagc
      901 acgccctcct tgtagacgcg gtcgcccacg gccaacatgt gatcgcggcg ggggatcggc
      961 agggcttgag caccggggat gagctgggcc aggtcttcgg cgctgccgcc gatgacgtcc
     1021 tcggtgccgc tggcgaccaa aaccgggcag gcaattttgg ccaccatctc gcggctgatc
     1081 ggctcgcgtg cgccgcgaat gcaggcggcg agcgccgcaa gatcgctgcc ggtcttgtcc
     1141 gcaaaggcgc ggaaggtgcg cgccgtcgga ttgaccacct cctcgatcga gcccacctcc
     1201 agggcatgcg cgatgggccc ggtgccggcc attggcctcg tcatattgcc gccgagcccg
     1261 ccaaaaatgg cgctgcgcac gcgttgcggg tgcgccaggg ccagaaacgc cgtgaggcgg
     1321 gcgcccatcg agtagcccat cacgtcggcg cgtgcgatgc cgaggtgatc gagcaggcgt
     1381 cgggcgtcct ccatcatcac cgacggctga taggcttgag gctcgtaaag cttctcgctc
     1441 tcgccgtgcc cacggttgtc gtaggcgatg acgcgatagc cgttgcgccg caacaagctg
     1501 acccagcccg gatcgagcca gttgtacctg acgctggagg cgaagccgtg cacgagcagc
     1561 accggatcac cgtttccctc atcgatgaaa cggatgcaaa tgccgtccga atcgaaggcc
     1621 tgcacagccc gctcctcctc agccaccctg cgaccttgcc aacccctatc atctggggcg
     1681 gcgagcacct acacttgggg cgttctcgag ctttttgagc agggccgccc catggcagtt
     1741 tcgatcatcc cgcatcttgc caacgatccg ggcgtggaaa agatctatat cggcgtcaga
     1801 gaattccagt gcatgggcgc gcgcccgccg catgaccacc cccatgtcta tctggatatg
     1861 ggggccgacc cgcagatttt gtgcccctat tgttcgacct tgtatatcca cgatgcccgg
     1921 ctgggcgccg atcaatccga tccgccgggc tgcctcgtca acgaagacca gccgcgacct
     1981 tgaggctcgt gtccggtcgg ccgcgtgggc aacgcacgac ccatcctggt actcggcggc
     2041 ggcatcgccg gcttggcgct ggcgctctcg ctcgcccgcc atggccgcgc caccgtcgtg
     2101 ctggagagcc gggcgcgctt tgagaccgaa ggtgccggca tccagctggg cgccaacggc
     2161 gtcaaggtgt tggcacggct gggactggcc gaacggctgc ggtccgaggt cggcgaaccg
     2221 gccagtctcg tcgtctttga cggccgctcg gcccggcgcc tggcgcagct gccgctcggg
     2281 agttggttcg cggcgcgtca tggcgccccc tactggacca tgcaccgtgc cgatctgcat
     2341 gcggcgctca ccgcaacggc ggcgcaacag ccgcatctcc tgttgcgcgc caattacgcg
     2401 ctcaaggcgg tgcggcaaga gacctccggc gtgctggccg aagacagggg aggcgagcgc
     2461 ctggcgggcg aggcgttgat tggcgcggac ggcctatggt ccgcggtgcg gcaaacgatt
     2521 tgcccggcgg ccaggccgca gttcgccgga gcgacggcgg cccgcgcgct gatcgcggca
     2581 agcgctgccg gcggcctggc cgagccgcac gtggggctgt ggctcgggac gggcgccaac
     2641 gtcgtacact atcccgtacg tgccggccgc gaagtcgccg tcgtcatcat cgttcgcgaa
     2701 ccgtggctgg cgtccacggc ggaaagccgg gtcgatgcta gtcaggcctt ggctccactt
     2761 gccgccttcc accactcgct gaccgacgtg ctcggccgcg ccgtcgacta ccgcaaatgg
     2821 gcgctctatc gcctggccgc gctgccggcg tggtcctctg agcgcatcgg cctgatcggc
     2881 gatgccgccc accccatgct gccgcacctg gcc
//
LOCUS       DASTMT010000023         4172 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_3946954, whole genome shotgun sequence.
ACCESSION   DASTMT010000023 DASTMT010000000
VERSION     DASTMT010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4172
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3946954"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..375
                     /locus_tag="VFR00_00540"
     CDS             <1..375
                     /locus_tag="VFR00_00540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0157762.1"
                     /translation="LENVVLIVAFAGYATTTSATLAGLLFVIDGVFFTLTLAQKTYFQ
                     KIADPSDMAATTSVAFTINHVAAVVVPVAFGTLGMIDPAIIFWLGACVACVSLSGSFL
                     VPRHPAPGHETVLASGRPLPAE"
     gene            complement(421..1467)
                     /locus_tag="VFR00_00545"
     CDS             complement(421..1467)
                     /locus_tag="VFR00_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007563809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-2,3-aminomutase-like protein"
                     /protein_id="HEU0157763.1"
                     /translation="MVGANTTLRKPGELEAAGLLPAGAARALEAVTDRYALAITPDML
                     RLIARGEPSDPIARQFVPDLAELNSVDGELADPFAEAQLSPVPGVVHRYGDRVLLKLT
                     HVCPVYCRFCFRREVVGPDGPPPLTAAALAAALAYIAATPRIWEVILTGGDPFMLSPR
                     RLTEVSRGLAGIDHVKVLRWHTRVPAVAPARVSAALLAALAAPGKTVMVVLHANHARE
                     LTSAARAACGRLVDAGIPMLSQSVLLKGVNDAVETLADLMRAFVEARVKPYYLHQLDP
                     APGTSHFRVPIVEGQALVRALRGRLSGLAQPTYVFDIPGGHGKVPIGPAYLNWDASTI
                     EDPRGAEHRLGERG"
     gene            1647..2021
                     /locus_tag="VFR00_00550"
     CDS             1647..2021
                     /locus_tag="VFR00_00550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0157764.1"
                     /translation="MRQGSSIAVAIALTYAGALGAVAQDEEVKASPSHWAVTGVAADD
                     VLHLRDVPSADSKSLAGIPFNARGLKNFGCRRGELPFDQWARMNEAQRRAANTKWCRV
                     EFHGKQGWVAGRFLKEDPTAAE"
     gene            complement(2024..3079)
                     /gene="epmA"
                     /locus_tag="VFR00_00555"
     CDS             complement(2024..3079)
                     /gene="epmA"
                     /locus_tag="VFR00_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009491021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P lysine aminoacylase EpmA"
                     /protein_id="HEU0157765.1"
                     /translation="MPPQSPSPWWNPRNHADRRPFLLARGRIMTAIRRYFSDQGFLEV
                     DTSPLQVSPGNEAHLHAFTTRLIDPDGTQHSFYLHTSPEFACKKLLAAGEERIFTFAP
                     VFRNRERGALHHPAFTMLEWYRARRPYDELMQDCASLLRLAAEAAGTTLLRHGTRSAD
                     PLAPLDRLSVAEAFRRHAGIDLLATLAGPALDAAALAAAATAAGVRVAADDTWGDIFS
                     RLLLAKVEPHLGDGRASILDQYPAELAALARPAAGDPRVAERFELYACGVELANAFGE
                     LTDAAEQRRRLEAEMREKERRWGERYPIDEDFIAALAQMPPAAGGALGLDRLVMLATG
                     ATRIEQVLWTPVAARGA"
     gene            3202..3771
                     /gene="efp"
                     /locus_tag="VFR00_00560"
     CDS             3202..3771
                     /gene="efp"
                     /locus_tag="VFR00_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010968469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEU0157766.1"
                     /translation="MVKVIASSVRKGNVLEVDGKLCVVMKAENIHPGKGTPVTHLDMR
                     RISDGVKITERYRTTDQVERAYLEDKTYNYLYEDDTGYTFMDPETYDQITVPKDLLGD
                     QSAYLQESMPVTLSLHEGVPVAIELPPRVTLEIVEADPVVKGQTASSSYKPAMLSNGQ
                     RVMVPPHIQAGTRVVVSTDDGSYVERAKD"
BASE COUNT          677 a         1365 c         1463 g          667 t
ORIGIN      
        1 ctcgagaacg tggtcttgat cgtggcgttt gccggctatg ccacgaccac gagcgcgaca
       61 ttggccggcc tcctgttcgt catcgatggc gtgttcttca cgctcaccct ggcgcagaag
      121 acctattttc agaaaatcgc cgacccctcg gatatggcgg ccaccacctc ggtcgccttc
      181 accatcaacc atgtcgcggc ggtggtggtg cccgtggcgt tcggcaccct cggcatgatc
      241 gatcctgcca ttatcttttg gttgggcgcc tgcgtggcgt gtgtctcgct gagcggctcc
      301 ttcctggtgc cgcgccatcc cgcgccgggc cacgagacgg tcttggcgag cggcagaccg
      361 ctgccagcgg agtgatggcc gcgccggccg ccgagccagg gcgcgaacct cgctagccgc
      421 tcagccgcgc tcgccgaggc ggtgctcggc cccgcgggga tcttcgatcg tcgaggcatc
      481 ccaattgagg taggcgggac cgatcggcac cttgccgtgg ccgccgggaa tatcgaaaac
      541 gtaggtcggt tgcgccaggc cggagaggcg gccgcgcagg gcgcgcacga gcgcttggcc
      601 ctcgacaatc ggcacgcgga agtgactggt gcccggggcc ggatcgagct ggtgcagata
      661 atagggtttg acgcgcgcct caacgaaggc ccgcatcagg tcggccagtg tttcgaccgc
      721 gtcgttgacg cctttcagga gcacgctctg gctcagcatg ggaatgccgg catcgaccag
      781 ccggccacag gcggcgcgcg cggcagacgt caactcacgc gcatggttgg catgcagcac
      841 caccatcacc gtcttgccgg gcgccgccag cgccgccagg agcgccgcgc tgacccgggc
      901 cggagcgacg gcggggaccc gcgtgtgcca gcgcaagacc ttgacatggt cgatgccggc
      961 gagcccgcgg ctgacctcgg tcagccggcg cggcgagagc atgaagggat cgccgccggt
     1021 caggatgacc tcccagatcc tgggagtagc ggcgatgtag gcgagcgcgg cggcgagggc
     1081 ggccgccgtc agcgggggcg gcccgtccgg gcccacgact tcgcggcgaa agcaaaaccg
     1141 gcaatagacc ggacagacgt gcgtcagttt caggagcacc cggtccccat agcggtgcac
     1201 cacccccggc accggtgaca gctgggcttc cgcgaagggg tcggccagct cgccgtcgac
     1261 gctgttgagt tcggcaaggt ccggcacgaa ttggcgcgca atcgggtcgc tcggctcgcc
     1321 ccgcgcgatg agccgcagca tgtccggtgt gatggcaagc gcataacgat cagttaccgc
     1381 ctcgagcgcg cgcgcggcgc cggcgggaag aaggcccgcc gcttccaatt cgcctggctt
     1441 gcgcagggtg gtgttggcac cgaccacgct gggatccctt cgcctgtggt gcggagcctt
     1501 taggcctgga tgggattggc ccgcaaggcc tatttcggca gaggcggcaa ggggcgcggc
     1561 tggcggtcgc ggcgggcgga ttgccatcgg cgcggcacat tcttggcgcc cagctgcggc
     1621 ccatcgggtt cgggggtaaa ccacgcatgc gtcaaggctc cagcatcgcc gttgcgatcg
     1681 cgctcaccta cgccggcgcg cttggcgccg tggcccagga cgaggaggtc aaggcaagcc
     1741 cgagccactg ggcggttacc ggcgttgctg ccgacgacgt tttgcatctg cgcgacgtgc
     1801 cctccgccga cagcaagagc cttgccggca ttcccttcaa cgcccgcggc ctgaaaaatt
     1861 ttggctgccg tcggggcgag cttcccttcg atcagtgggc gcgcatgaat gaggcgcagc
     1921 ggcgggcggc gaacaccaaa tggtgccggg tcgagtttca cggcaagcag ggctgggtgg
     1981 ccgggcgttt cctgaaggag gatcccaccg cggccgagtg aggtcaggcg ccgcgcgcgg
     2041 caaccggtgt ccacagcacc tgctcgatgc gggtggcccc ggttgcgagc atcaccaggc
     2101 ggtccagtcc cagggcgccg ccggccgccg gcggcatctg ggcgagcgcg gcaatgaagt
     2161 cctcgtcgat cggatagcgt tcgccccagc gccgctcttt ttctcgcatc tcggcctcca
     2221 ggcggcgccg ctgctcggcc gcatcggtca gttcgccgaa ggcattggca agttcgaccc
     2281 cgcaggcata aagctcgaag cgctcggcca cgcgcgggtc gccggccgcg ggccgtgcca
     2341 gggccgcaag ctcggcaggg tattggtcga ggatgctggc gcgaccgtcg ccgaggtggg
     2401 gttcgacctt ggcaagcaag aggcggctga agatgtcgcc ccaggtatcg tccgcagcca
     2461 cgcgcacgcc cgcagcggtt gccgcggcgg cgagcgcggc ggcgtctagt gccgggcccg
     2521 ccagggttgc cagcagatcg atgccggcgt ggcggcgaaa ggcctccgcc acgctgaggc
     2581 ggtcgagcgg ggccaacgga tcggcgctgc gcgtgccgtg gcgcaggagg gtcgtgccgg
     2641 ctgcctccgc ggcgagccgc agcaggctcg cacaatcctg catgagctcg tcataggggc
     2701 ggcgcgcccg gtaccattcg agcatggtga aggccggatg atgcagcgcg ccccgctcgc
     2761 gattgcggaa tacgggcgcg aaggtaaaaa tccgttcttc gccggcggcc aacagcttct
     2821 tgcacgcgaa ttcgggggag gtgtgcaaat agaagctgtg ctgggtgcca tcgggatcga
     2881 tcaggcgggt cgtgaaggcg tgcaggtggg cctcgttacc gggtgaaacc tgcagggggc
     2941 tggtgtcgac ttccaggaaa ccctgatcgg agaagtagcg gcgaattgcc gtcatgatac
     3001 ggccgcgcgc caataaaaag gggcggcggt cggcatggtt ccgcgggttc caccacggac
     3061 ttgggctttg gggcggcagg cgtttgctcc ttggcgcggt tcgaaaaggc cgctagaaag
     3121 cgccagcttt gaggactcat ggcgagcctg ccgtcgctcc tggcgcacgc ggccaagcct
     3181 gctaatcggg agagatcgac ggtggtgaag gttatcgcaa gctcggtgcg caaaggcaac
     3241 gtgctggaag tggacggcaa actgtgcgtc gtcatgaagg cggagaatat ccacccgggc
     3301 aagggcacac cggtaacgca cttggatatg cggcgcattt ccgatggcgt gaagatcacc
     3361 gaacgctatc gcaccaccga ccaggtcgag cgcgcctatc tcgaggacaa gacgtataat
     3421 tatctttatg aggacgacac gggctacacc ttcatggatc ccgagaccta cgatcaaatc
     3481 acggtgccga aggatctgct gggtgaccaa tccgcctatt tacaggagag catgcccgtc
     3541 accttgagcc tgcacgaggg cgtgcccgtg gcaatcgagc tgccgccgcg cgtgaccctg
     3601 gaaatcgtcg aggccgatcc cgtcgtcaaa ggtcagaccg cctcctcctc ttataagccg
     3661 gccatgctct ccaacggcca gcgcgtgatg gtgccgccgc acatccaagc cggcacgcgc
     3721 gtcgtcgtct ccaccgacga cggctcctac gtcgagcgcg ccaaggacta ggcggcagcc
     3781 gaacgccccg cttggcttca tctcacgtgc gcttcatctc acgtgcgcgc cttgtcgcgg
     3841 ctcgagcggc gcgatccggc gccgagccaa tcggtgcaac ttgcgctggg caagcccatt
     3901 cactgccgag ctgaagccgc gatcggccgc gcatccggat gcagacgatg ctcctcgtgc
     3961 catgctgccg ccgggcgcct gccgagccgg ccttggcggc gcggggctcg cgctgccgcc
     4021 ggcgcggcga tcgtgctatg cctgcgctcg cagccaagtc acgagcatgc gtcatgatcg
     4081 atcttgcggc tatccgcccg gggctcacgg gcacggcgga actgattgtc ggccccgagc
     4141 acacggcgcc ccgcgtcggc tcggggcgca tc
//
LOCUS       DASTMT010000024         2281 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_4926890, whole genome shotgun sequence.
ACCESSION   DASTMT010000024 DASTMT010000000
VERSION     DASTMT010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2281)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2281)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2281
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4926890"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..649
                     /locus_tag="VFR00_00565"
     CDS             <1..649
                     /locus_tag="VFR00_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HEU0157767.1"
                     /translation="SSPEILVRVRDGEVTIRPLAGTRRRGGTPEEDLALEKELLADPK
                     ERAEHLMLLDLGRNDVGRVAKIGSVRVKESFAIERYSHVMHIGSHVVGALDERHDVVD
                     ALMAGFPAGTLSGAPKVRAMEIIDELEKEKRGPYGGCVGYFSADGEMDSCIVLRTALV
                     KDGAIYVQAGAGIVADSEPDAEQLECELKARALFKAAEEAVRFAARSKRGNRPGP"
     gene            674..1807
                     /locus_tag="VFR00_00570"
     CDS             674..1807
                     /locus_tag="VFR00_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011090618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU0157768.1"
                     /translation="MRAGTSTYQAALPPGIRARSIPNGNGLLMHVLEAGEPDRGRPCL
                     LLLHGFPELAYSWRKLMLPLAAAGFYVVAPDQRGYGRTTGWDAAYDGDLAPFRVINLV
                     RDTLGLVSALGYRSVAAVIGHDFGAPVAAWCALLRGDVFRSVVLMSAPFVGPPELPAP
                     SPPAPPTPDIHAALAALEPPRKHYHWYYSTREADANMRHCPQGLHAFLRAYYHYKSAD
                     WTGNAPFPLTAWTAPELAKLPTYYVMRLSDGMAETVAPEMPSPEAVAACHWLSEAELC
                     VYSREFQATGFQGGLNWYRCRTSGRFEAEQQVFAGRRIEVPALFIAGKSDWGAFQVPG
                     ALDKMQNAACARFLGSHFVEGAGHWVQQEQPAAVLELLGTFLS"
     gene            1973..>2281
                     /locus_tag="VFR00_00575"
     CDS             1973..>2281
                     /locus_tag="VFR00_00575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0157769.1"
                     /translation="MLSDTIERPQVSNTVVASHFPTLFMLREYANCGGLNEKLERLML
                     RLEKETANIAPATSNIGGYHSDTTLFSRKEPEIAALRELVQAALHEYVPKFLEANCHA
                     P"
BASE COUNT          372 a          795 c          752 g          362 t
ORIGIN      
        1 ctcctcaccc gagatcttgg tgcgcgtgcg cgacggcgag gtcaccatcc ggccgctcgc
       61 cggcacgcgc cggcgcggcg gcacgccgga agaggatctg gccctggaaa aggagctgct
      121 cgccgatccc aaggagcgcg ccgagcatct gatgctgctc gatctcggac gcaacgatgt
      181 gggacgcgtc gccaagatcg gctcggtgcg cgtcaaggag agctttgcca tcgaacgcta
      241 cagccacgtc atgcacatcg gcagtcacgt ggtgggcgcg ctcgatgagc gccacgacgt
      301 ggtggacgcg ctgatggcgg gcttccccgc cggcaccctc tccggtgccc ccaaggtgcg
      361 cgccatggaa atcatcgacg agctcgagaa ggaaaagcgc gggccctacg gcggctgcgt
      421 gggctatttc tcggccgatg gggagatgga tagctgcatc gtgctgcgca ccgccctggt
      481 gaaagacggc gccatctacg tccaggccgg cgccggcatc gtcgccgaca gtgaacccga
      541 tgccgagcag ctcgagtgcg agctgaaagc ccgcgccctg ttcaaggcgg ccgaggaggc
      601 ggtgcgcttt gcggcgcgca gcaagcgcgg caaccggccg gggccctgaa cgcaaggcct
      661 gaagggggat agcatgcgcg ccggcaccag cacctatcag gccgccctgc cgccggggat
      721 ccgcgcgcgc tccatcccca atggcaacgg cctcctcatg cacgtgctgg aggccggcga
      781 gcccgatcga ggccggcctt gcctgctgct gctgcacggc tttcccgagc ttgcctatag
      841 ctggcgcaag ctgatgctgc cgctggcggc ggcgggtttt tacgtggtcg cccccgacca
      901 gcgcggctat ggccgcacca cgggctggga cgctgcctac gatggcgacc ttgcgccgtt
      961 ccgcgtcatc aacctggtgc gcgacacctt aggcctggtc tcggcgctcg gctatcgctc
     1021 ggtcgcggcc gtgatcggtc atgatttcgg ggcgccggtg gcggcctggt gcgcgctgtt
     1081 gcggggcgat gtgttccgct ccgtggtgct gatgagcgcg cccttcgtcg gcccgccgga
     1141 gctgcccgcg ccgagcccgc cggcgccacc cacccccgat attcacgccg cccttgccgc
     1201 cctggagccg ccgcgcaagc actatcattg gtactattcg acgcgcgaag ccgacgccaa
     1261 catgcggcat tgcccgcagg gcctgcacgc cttcctgcgc gcctactacc actacaagag
     1321 cgccgactgg acggggaacg cgcccttccc gctcaccgcc tggaccgcgc ccgagctcgc
     1381 caagctcccc acctactacg tcatgcggct cagcgatggc atggccgaaa cggtggcgcc
     1441 ggaaatgccg agccccgagg ccgtcgccgc ctgccattgg ctcagcgaag cggagctttg
     1501 cgtctatagc cgcgagttcc aagcgaccgg ctttcagggc gggctcaact ggtaccgctg
     1561 ccgcaccagc ggccgcttcg aggccgagca gcaagtcttt gccggccgcc gcatcgaggt
     1621 ccctgccctg tttattgcgg ggaagagcga ctggggcgca ttccaggtgc caggcgccct
     1681 ggacaaaatg caaaacgccg cctgcgcccg ctttttaggc agccatttcg tcgagggtgc
     1741 cgggcactgg gtgcaacagg aacagcccgc ggcggtcctc gagctgttgg ggaccttctt
     1801 gagctgacgg gcgactcaag gtcctctacg gcgcaagcgc aaaatcggcg tcgaagcaag
     1861 atggcttcct gcatggtagc ccctcggcaa gcgccgctgc tctcgctgcg ccagcacact
     1921 aggctgccgc agcgctgcgc tcggccgggg tggccgcatc gggaggccag gcatgctgag
     1981 tgacaccatc gagcgaccgc aggtctccaa tacggtcgtt gcctcgcatt tcccgaccct
     2041 gttcatgctg cgcgagtatg ccaactgcgg cgggctcaac gaaaaactcg agcggctgat
     2101 gctgcgcctg gaaaaggaaa cggccaatat cgcccccgcc accagcaaca tcggcggcta
     2161 tcactccgac accacgctgt tctcgcgcaa agagccggag attgccgcgc tgcgcgagct
     2221 ggtacaggcg gcgttgcacg aatacgtgcc gaaattcctc gaggccaact gtcacgcccc
     2281 g
//
LOCUS       DASTMT010000025         4259 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_1322026, whole genome shotgun sequence.
ACCESSION   DASTMT010000025 DASTMT010000000
VERSION     DASTMT010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4259)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4259)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4259
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1322026"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(87..1274)
                     /locus_tag="VFR00_00580"
     CDS             complement(87..1274)
                     /locus_tag="VFR00_00580"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008960292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEU0157770.1"
                     /translation="MVQERTGPLSRFKVIDLTRARAGPTAARHFADWGADVIKVEMPL
                     ESDEDGLGGSRHGPDFFNLHRNKRSLALNLKTPEGVAILKELAADADVLIENYRPDVK
                     KRLGIDYETLRALNPRLVYVSLSGFGQTGPYANRPGFDQVAQGMGGLMSITGLPGQGP
                     VRVGIPIADLSSGNYAAMGALVALLEREVSGEGQWVKTSLLQAQISMLDFQAARWTVA
                     RDVPKQAGNDHPTSIPTGVFPTSDGHINIAASGQHIFARMCKAMAAERLLEDPDFTTG
                     EKRSQHRARLNTEIGSFTKKYTSLDLVERLNAAGVPSGPIYDIGQMFADPQVKHVGMA
                     VPMPHPTRKDAAIVNQAVELSRTPAAINRPTPGLGEHSEEILAGLGYSSSDIAALRSK
                     KVI"
     gene            complement(1329..2300)
                     /locus_tag="VFR00_00585"
     CDS             complement(1329..2300)
                     /locus_tag="VFR00_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012043232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEU0157771.1"
                     /translation="MKAAVLGESGLEIRDVAKPVPGPSQVLVKVRAAGLNRADLIMAS
                     GQRHGNAGGVGSVLGLEFAGEVEAVGSEVTTVRPGERVMCSGNGGYAEYAVADWGRVS
                     TIPANNMGYEAAATLPVALQTMHDAVVTNGRLKPGETVLIQGASSGVGLMGLQIAKLK
                     GARLVIGTSTNAARRARLHEFGADLAVDTTAANWSARVLEATAGKGVDLIVDQVSASV
                     ANENLKATAILGRIVNVGRLGGFKGEFDFDLHALRRIDYIGVTFRTRTPEEVREIVRR
                     MRADLWGEIEAGRLRLPIDRTFPLDEAKAALAHMRANAHFGKIVLTV"
     gene            complement(2297..3184)
                     /locus_tag="VFR00_00590"
     CDS             complement(2297..3184)
                     /locus_tag="VFR00_00590"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU0157772.1"
                     /translation="MAMPDSAPVRRRGSLERPGARIAYEVTGAGRPIVFAHGLGGNHA
                     SWWQQVAYFAPSYTCVTFAHRGFYPSSAIAGGPDPNAYAEDLAALIDHLGLGEVCLVA
                     QSMGGWTAIEYALRPEAKLAALVLAASTGTIDPARLWQQPHERDRLARWQADSNAAGR
                     ELWRRGIHIACGARMAREQPALHLIYRHIDEMNAPIDKEALRARLLGARTRAPAELAA
                     IACPTLLISGAEDIVMPPFAADAMAAALPRAEVAHIAEAGHSAYFERAQVFNATLADF
                     LARCGWPSLEREPCLESNA"
     gene            complement(3249..>4259)
                     /locus_tag="VFR00_00595"
     CDS             complement(3249..>4259)
                     /locus_tag="VFR00_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007756331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEU0157773.1"
                     /translation="WAEAWEELKALAELLAIPVCTSLEGKSAFPETHPLALGSGGLAI
                     PGSVHHFLKEADIIFGIGCSFGQTNFGVAMPKGKTIIHATLDAADINLNVPCALGILG
                     DAKLVLAAILDEVRRSQKSPRDSGKVAAEIREVHDAWLAKWLPKLTEDGTPLNPYRVL
                     WDLQKTVDVANTIITHDAGSPRDQLSPFWRPTTPLSYLGWGKTTQLGYGLGLAMGAKL
                     AKPDKLCVNVWGDAAIGFTGMDFETAVRERIPILSILLNNFSMAIELKVMPISTEKYR
                     ATDISGDYAAMARAFGGFGERVEKPADIVPAIKRGIAATERGQPALLEFITSKEVNVS
                     RF"
BASE COUNT          740 a         1357 c         1405 g          757 t
ORIGIN      
        1 tcttcgacgc ggccgatcat cttcgctgtg ccggtatcca cgagtgctgc ctccggatta
       61 gggcgccgct tggcgccacc ttcgtcctaa atgaccttct tgctgcgcag tgccgcaatg
      121 tcgctgctgc tgtagcccag ccccgccaaa atctcttcgc tgtgctcacc gagccccggg
      181 gtgggccgat tgatggccgc gggcgtgcgc gacagctcca cggcttgatt gacgatggcg
      241 gcgtccttgc gcgtgggatg cggcatcggc acggccatgc cgacgtgttt gacctggggg
      301 tcggcgaaca tttggccaat gtcatagatc ggcccggacg ggacgcccgc cgcgttcaag
      361 cgctccacca ggtccaagct cgtgtatttc ttcgtgaagc tgccgatttc ggtattgagg
      421 cgcgcacgat gttgcgagcg tttctcgccg gtggtgaagt cgggatcctc gagcagccgc
      481 tcggcggcca tggccttgca catgcgggca aaaatatgct gacccgacgc ggcaatgttg
      541 atgtgtccgt cgctggtcgg aaacacgccg gtcgggatcg aggtgggatg atcgttgccc
      601 gcttgcttgg gcacatcgcg cgccacggtc caacgggccg cctgaaaatc cagcatcgag
      661 atctgcgctt gcagcagcga ggtcttgacc cactgcccct cgccagagac ctcccgctcc
      721 agcaaggcaa cgagggcgcc catcgccgcg tagttgccgc tggaaagatc ggcaatcgga
      781 atgccgaccc gcaccggacc ctgacccggc agtccggtaa tggacatcag gccgcccatg
      841 ccctgggcga cttgatcgaa gccgggccga ttggcgtaag ggccggtctg gccgaagccg
      901 gatagactga cgtagacgag ccgcgggttc agcgcgcgca aggtctcata gtcgatgccc
      961 agccgcttct tgacgtcggg tcgatagttc tcgatcagca cgtcggcgtc agccgcaagc
     1021 tctttgagga tggcgacgcc ctcaggcgtc ttcagattga gcgcaaggct gcgcttgttg
     1081 cgatgcagat taaaaaaatc cggaccatgc cgcgagccgc cgaggccatc ttcatccgac
     1141 tcgagcggca tctcgacttt gatgacgtcg gcaccccaat ccgcgaaatg ccgcgccgcg
     1201 gttggcccgg cgcgggcccg ggtcagatcg attaccttga agcgggacag cgggcctgtg
     1261 cgctcttgga ccattagcaa atgcgccttt ttctggatgc gtcgagggat cagcatacgc
     1321 cggtgcggtc ataccgtcag cacgatcttg ccgaagtggg cgttggcccg catgtgcgcc
     1381 agcgctgcct tggcctcatc gagcgggaag gtgcgatcta tcggcagccg caaccggccg
     1441 gcctcgatct cgccccacaa atcagcgcgc atgcgccgca cgatctcgcg cacctcttct
     1501 ggcgtgcgcg ttcgaaaggt gacgccgatg tagtcgatcc gacgcaaggc gtgcagatcg
     1561 aagtcaaatt cacccttgaa gccaccgagc cgcccgacat tgacgatgcg cccgaggatg
     1621 gcggtggctt tcaagttctc gttggcgacg ctggcggaga cttggtcgac gatcaaatcg
     1681 acacctttgc cggcagtggc ctccaggacg cgggcggacc aattggctgc ggtggtgtcg
     1741 accgccaggt cggcgccgaa ctcgtgcagc cgcgcgcgtc gcgccgcgtt ggtggaggtg
     1801 ccgatgacga ggcgggctcc cttgagcttg gcgatttgca ggcccatcag gcccacaccc
     1861 gagctggcgc cctggatcag cacggtttca ccgggtttga ggcggccgtt cgtgacgacg
     1921 gcatcgtgca tggtctgtag cgccaccggc agcgtcgcgg ctgcctcgta gcccatgttg
     1981 ttggcgggaa tggtcgagac gcggccccag tccgcaacgg cgtattcggc atagccgcca
     2041 ttgccggagc acatcacgcg ctcacccggt cgcacggtgg tgacctcgct gccgacggcc
     2101 tcgacctcgc cggcaaactc gagcccgagc acgctgccca cccccccggc gttgccgtgg
     2161 cgttggccgg aagccatgat caggtcggcc cgattgagac ccgctgcacg cactttcacc
     2221 agtacttggg agggacccgg aaccggtttt gccacgtcgc ggatttcgag cccgctttcg
     2281 cccagcacgg cagctttcat gcgttgctct ccaagcaagg ttcacgctca agggatggcc
     2341 agccgcagcg tgcgaggaaa tccgcaagcg ttgcgttgaa aacctgcgcc cgctcgaaat
     2401 aagcggaatg cccggcctcg gcaatatggg ccacctcggc gcgcggcagc gcggccgcca
     2461 tggcatcggc ggcaaagggt ggcataacga tatcctcagc ccctgaaatc aacagcgttg
     2521 ggcaggcgat ggccgcgagt tcggcagggg cgcgggtgcg cgcccccagc agccgggcgc
     2581 gcaaggcttc cttgtcgatc ggcgcattca tctcgtcgat gtggcgatag ataagatgta
     2641 gcgctggctg ctcgcgggcc atgcgcgcgc cgcaggcaat atggatgccg cgccgccaaa
     2701 gctcgcgacc ggctgcgttg ctgtccgcct gccagcgcgc gaggcgatcg cgctcgtgcg
     2761 gctgttgcca caagcgggcc gggtcgatcg ttcccgtgct ggcggcgagc acaagggcgg
     2821 cgagcttggc ctctggccgc aaagcgtatt cgatggcggt ccagcccccc atcgattggg
     2881 caacaaggca cacctcgccc aggccgagat gatcgatgag ggcggcgagg tcctcggcat
     2941 aggcgttggg atcgggcccc ccggcgatcg ccgaggaggg gtaaaagccg cggtgggcga
     3001 aggtcacaca ggtatagctc ggggcgaagt aggcgacctg ttgccaccag gaggcatgat
     3061 tgccgccgag gccatgcgca aacacgatgg gccgacctgc gccggtcact tcgtaagcga
     3121 tgcgcgcccc cggccgctca aggctgccgc ggcgacgcac gggcgcgctg tcaggcattg
     3181 ccatggccgg gccgcctaag gacaaaggca accgtggcga aggtcggccg ctgcagcatg
     3241 ggcgaggctc agaacctgga aacgttgact tccttgctgg tgatgaactc cagcagcgcc
     3301 ggctgccccc gctcggtcgc ggcgatgcca cgcttgatcg ccggtacgat gtcggcgggt
     3361 ttctccaccc gctcgccgaa gccgccgaag gcccgtgcca ttgccgcgta gtcgccggaa
     3421 atatcggttg cacgatactt ctccgtcgag atcggcatca ccttgagctc gatcgccatg
     3481 gagaaattat tgagcaggat ggagaggatc ggaatgcgct cccgcaccgc ggtctcgaaa
     3541 tccatgccgg taaaaccgat cgccgcatcg ccccagacgt tgacgcaaag cttgtccggt
     3601 ttggcgagct tggcacccat ggcgaggccc agaccgtagc cgagctgcgt ggtcttaccc
     3661 cagcccagat aggagagcgg cgtggtgggt cgccagaagg gcgagagctg gtcacgcggg
     3721 cttcccgcat catgcgtgat gatggtattg gcgacatcga cggtcttctg caggtcccac
     3781 aacacgcgat agggattgag cggggtgccg tcctcggtga gctttggcag ccatttggca
     3841 agccaagcat cgtgcacctc gcggatttcc gcggccacct tgccggagtc acgcgggctc
     3901 ttctggctgc ggcgcacctc atcaaggatg gcggccaaca ccagtttggc atcgcccagg
     3961 atgcccaagg cacagggcac gttgaggttg atatccgcgg catcaagcgt ggcgtgaatg
     4021 atggtcttgc ccttgggcat ggccacgccg aagttggttt gcccgaaact gcaaccaatg
     4081 ccgaaaatga tgtccgcttc cttgaggaag tgatgcaccg agcccggtat ggcgagccca
     4141 cccgagccga gcgcgagcgg gtgcgtctcg ggaaaggcgc ttttgccctc gaggctggtg
     4201 cagacgggaa tggccaagag ctcggccagc gccttcagtt cttcccaggc ctcggccca
//
LOCUS       DASTMT010000026         2286 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_665418, whole genome shotgun sequence.
ACCESSION   DASTMT010000026 DASTMT010000000
VERSION     DASTMT010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2286
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_665418"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..350)
                     /locus_tag="VFR00_00600"
     CDS             complement(<1..350)
                     /locus_tag="VFR00_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014892943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphatase family protein"
                     /protein_id="HEU0157774.1"
                     /translation="MNSVRSPMAAAILRHLAGRRAYVESAGVRAGGLDAFAVAAMDEI
                     GIDISQHAPRTLRDLHDTSFDLIVSLSPEAHHQALELTRTMAVEVEYWPTFDATLLIG
                     SAGREQILQAYRDVR"
     gene            complement(408..905)
                     /locus_tag="VFR00_00605"
     CDS             complement(408..905)
                     /locus_tag="VFR00_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012113252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0262 family protein"
                     /protein_id="HEU0157775.1"
                     /translation="MFEPPASDANPKARLSAITLDSSSIKRGSSGIEHEREVAIFDIL
                     ESNFFSLEGRDEGPYTLNLSIAEDRLVLAVGQQASDSVFTVVLSMTPLRRVMKDYFIV
                     CESYYRAIRTAPPSRIQAIDMGRRALHDEGSRVLLERLKGKITVDNDTARRLFTLICA
                     LHWKG"
     gene            complement(919..2220)
                     /gene="hisD"
                     /locus_tag="VFR00_00610"
     CDS             complement(919..2220)
                     /gene="hisD"
                     /locus_tag="VFR00_00610"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HEU0157776.1"
                     /translation="MPVRLATTDANFDAAFASFLANKREVSPDVEDSVRAIIAEVRQR
                     GDAALVEYTRRFDQFDLSSLGVRMPEAEIRAAARGCDAATLAALAMAKARIEAYHARQ
                     LPKDEAFTDGVGAELGWRWSAIESVGLYVPGGLASYPSSLLMNAVPAKVAGVPRIAVA
                     VPAPKGEVNPYVLAAADIVGISEIYRIGGAQAVAAFAFGSQSVAPVAKIVGPGNAYVA
                     AAKRQVFGTVGIDAIAGPSEVLIIADGQNDPAWIAADLLAQAEHDSAAQAILMTDDAA
                     FAARVEDEVERQLVRLPRRDIAEASWREYGAVIILATLEHAPALADRIAAEHVQIVTR
                     DPDQLARRIRNAGAFFLGAQTPEVIGDYLAGSNHVLPTARSARFASGLGVYDFMKRTS
                     ILRLDAAALRGLAAPAMLLARAEGLAAHERSVALRLDPAAD"
BASE COUNT          420 a          727 c          768 g          371 t
ORIGIN      
        1 cggacgtcgc ggtaggcctg aaggatctgc tcgcggccgg cgctgccgat caaaagcgtg
       61 gcatcgaagg ttggccaata ctcaacctcc acggccatgg tgcgggtaag ctccagcgcc
      121 tgatggtgcg cttcggggga cagcgagacg atcaaatcga aggaggtatc gtgcagatcg
      181 cgcagcgtgc gcggggcgtg ctgagaaatg tcgatgccaa tttcgtccat cgccgccacg
      241 gcaaaggcgt ccaatcctcc ggcgcgcacg cccgccgatt ccacgtaggc gcgccgcccg
      301 gcgaggtgcc ttagaatcgc cgcggccatc ggcgagcgca ccgagttcat cgtgcaggcg
      361 aataagaccg cgcccggcag ctcttggccc cccgcgcccg ccacctctca tcctttccag
      421 tgcagcgcgc agatcagcgt gaagaggcgc cgcgccgtat cattgtcgac ggtgatcttg
      481 cccttgagcc gttccagcag cacgcgactg ccctcgtcat gcaaggcccg ccgccccatg
      541 tcgatggctt ggatgcgcga aggtggcgcg gtgcggatag cccggtagta gctctcgcac
      601 acgatgaaat agtccttcat gacgcggcgc agcggcgtca tggacaacac caccgtgaat
      661 acgctgtcgc tcgcctgctg gccgacggcc aagacgagcc ggtcctcggc gatggaaagg
      721 ttgagcgtat agggaccttc gtcgcggccc tccaggctga agaaattgct ctccaggata
      781 tcgaagatgg cgacctcgcg ttcgtgctcg atgccggagc tgccccgctt aatggagctg
      841 ctgtcgagcg tgatggcgct gagccgagcc ttgggattgg catcgcttgc cggcggctca
      901 aacattgagg cctcaaggtc aatcggcggc agggtcgagg cgcaacgcca ccgagcgctc
      961 gtgcgcagca agaccttcgg cacgggcaag aagcatcgcc ggtgccgcga gccctcgcaa
     1021 cgcggctgcg tcgaggcgca ggatcgaggt gcgcttcata aagtcataaa cgccgagccc
     1081 cgaggcaaag cgcgcgctac gcgccgtcgg tagcacgtgg ttggagccag ccaggtaatc
     1141 gccgatcacc tccggagttt gggcaccgag aaaaaaggcg cccgcgttgc ggatgcgacg
     1201 ggcgagctgg tcgggatcac gcgtcacgat ttgcacgtgc tcggcggcaa tgcggtcggc
     1261 cagggccggg gcatgctcga gcgtcgccag gatgatcacc gcaccgtact cgcgccagct
     1321 cgcttcggcg atgtcgcgac ggggcaagcg gacgagctga cgctcaacct catcttcgac
     1381 gcgggcggca aaggcggcat catcggtcat cagaatggct tgtgcggcgc tgtcgtgctc
     1441 ggcctgcgcc aacaggtcgg ctgcgatcca ggcgggatcg ttctgcccat cggcaatgat
     1501 gagcacctcc gaggggccgg cgatggcgtc gatgccaacc gtgccgaaca cctggcgctt
     1561 ggcggcggcc acataggcgt ttccggggcc gacgatcttg gcgacgggtg caacactttg
     1621 gctaccgaag gcaaaagcgg ccacggcctg ggcgccgcca attcggtaga tttccgagat
     1681 gccaacgata tcggcggcgg ccaacacgta gggattgacc tcacccttgg gcgccggcac
     1741 agcaacggcg atccgcggca caccggccac cttggccggg accgcgttca tcaagagcga
     1801 gctcgggtaa ctggcaaggc cgccggggac gtaaaggccg actgactcga tggccgacca
     1861 acgccaacct agctcggcgc cgacgccatc ggtaaaggcc tcgtctttgg gcagctggcg
     1921 tgcgtggtag gcctcgatcc gggctttggc catggccaag gccgcaagcg tggcggcatc
     1981 gcagccgcgc gcggccgcgc ggatctcggc ttccggcata cgtacgccca ggctcgagag
     2041 atcgaactga tcgaagcgac gtgtgtactc caccagggcc gcatcgccgc gttggcgaac
     2101 ctcggcgatg atcgcccgta cgctatcctc gacgtccggc gaaacctcgc gcttgttcgc
     2161 caagaagctg gcgaacgcgg cgtcgaaatt tgcatctgtg gtcgcgagac gaacgggcat
     2221 ggccaagttt cgatgacgat caggtggagc ggtccttgcc gggaccagat gaaattgggc
     2281 tgccgt
//
LOCUS       DASTMT010000027         2785 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_6240318, whole genome shotgun sequence.
ACCESSION   DASTMT010000027 DASTMT010000000
VERSION     DASTMT010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2785)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2785)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2785
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6240318"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..656
                     /locus_tag="VFR00_00615"
     CDS             <1..656
                     /locus_tag="VFR00_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEU0157777.1"
                     /translation="KLAAEVLPQGFTFEWTTLAYQQLRAGNTALFAFLLAVVFVFLVL
                     AAQYESLTLPLAVILIVPMCLIASITGVVWRGMDNNILTQVGFIVLIGLAAKNAILIV
                     EFAKQLEDQGRNRWAAAAEAARLRLRPILMTSLAFIFGVVPLVWAIGAGAELRQALGT
                     AVFYGMIGVTAFGLIFTPVFFVVSRWLAGLFARKPALTAPDAVTPSPLGSSDPVGQA"
     gene            complement(763..2322)
                     /locus_tag="VFR00_00620"
     CDS             complement(763..2322)
                     /locus_tag="VFR00_00620"
                     /inference="COORDINATES: protein motif:HMM:NF025068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M12 family metallo-peptidase"
                     /protein_id="HEU0157778.1"
                     /translation="MVFLLLLPGAGTALAQGGPGRSEAARPEKGSQAPADMVIISNMP
                     ERKGPIYNLLKKLFCKNNGAVTGAANSEVWSVPQGQTSRVSKWLEGLGMKVTKLREDW
                     NHILRRHEGPVTPAQQAMVDRMKAAPGTMGVHVLQAPDAAVSAFLMTNEIAYRPSVPA
                     ELQTTPSKEQAPSRVVLPITPTKSITLERVRYSSDERGCTWHGIVAETGESALLMRWH
                     DGHITGLVGYKGRIYTVASLGGQLQAVVEMDPRQMPPDHAAAKPTADPRADLRPDAPA
                     KLAVAAPPAVVAPLPDTERQALEAKQIVIDVMLLYTKRAASRYVRDPSDLLMMAIEQA
                     NDAFRNSGLGNISLRLVHTQAIDYDESDGDHFEHLYRMVDGVGPFAGVRQLRNEKKAD
                     IVGLILDDASGCGLSTRVAPDAEEAYFVVHHSCAAITISIAHEIGHILGARHDRSIDG
                     NDTPFAYGHGHVNGKWRDIMSYRQSCDGCLRIPYWSNPRVLYNGEPTGTANEDNARVI
                     LEQAERVSKFR"
BASE COUNT          457 a          906 c          902 g          520 t
ORIGIN      
        1 aaaagctggc ggccgaagtt ctgccgcaag ggttcacctt cgagtggacc acgctcgcct
       61 accagcagct cagggccggc aacaccgcgc tgttcgcctt cctgctggcg gtcgtgttcg
      121 tgttcctcgt gctcgccgca caatatgaga gcctgacgtt gcccctggca gtcattctga
      181 tcgtaccgat gtgtctcatc gcctccatca ccggggtggt ctggcggggc atggacaaca
      241 acattttgac ccaggtcggc ttcattgtgc tgatcggcct cgccgccaag aacgccatcc
      301 tgatcgtcga attcgccaag cagctcgagg atcaggggcg caaccgctgg gccgcggcgg
      361 ccgaagcggc caggctccgg ctcagaccca ttctgatgac gtcgcttgcc ttcatcttcg
      421 gcgtggtccc cctggtgtgg gcgatcggcg ccggcgccga gctcaggcag gcgttgggaa
      481 ccgcggtgtt ctacggcatg atcggcgtca cggccttcgg tttgattttt acgccggtct
      541 tcttcgtcgt cagccgctgg ctggcgggcc tgttcgcgcg caagccggcg ctcaccgcac
      601 cggacgccgt cacgccgtcg ccactggggt cgagtgatcc cgtgggacag gcttagttcg
      661 gagccgcggc cggcctcacg caagcgcgca ttgcacgcac tgatgccgtg cgccttgtcg
      721 gcatggctcg cagccaagcc gccgggaacg ccggcaaatg ggtcagcgga atttcgaaac
      781 gcgctcggcc tgctcgagga tgacccgggc gttgtcctcg ttcgccgtgc ccgtcggctc
      841 cccgttgtag aggacgcgcg ggttcgacca gtaggggatg cgcaagcagc catcgcagct
      901 ctgccggtag ctcatgatat cgcgccattt gccgttgaca tgaccgtggc cgtaggcaaa
      961 gggcgtgtcg ttgccgtcaa tgctgcggtc gtggcgcgcg cccaggatat gcccgatctc
     1021 gtgcgcgatc gaaatcgtga tggcggcaca ggagtgatgc acgacaaaat aggcctcctc
     1081 cgcatccgga gccacgcgcg ttgacaagcc gcagcccgag gcatcgtcga ggatcaggcc
     1141 gacgatatcc gccttcttct cgttgcgcag ctggcgcacc cctgcgaacg ggccgacccc
     1201 atcgaccatg cggtaaagat gttcgaagtg atccccgtcg gattcgtcgt aatcgatggc
     1261 ctgggtgtgc accaggcgca gggagatatt gccaagaccg ctgttgcgga acgcgtcgtt
     1321 ggcctgttcg atggccatca tcaggagatc gctggggtcg cgcacgtaac gactggcggc
     1381 tcgcttggtg tagagcagca tgacatcgat cacgatctgc ttggcttcca gcgcctggcg
     1441 ctcggtgtcc ggtagcggcg ctaccacggc gggtggcgcc gccacggcaa gcttggccgg
     1501 cgcatcgggc cgcaaatcgg cgcggggatc cgccgtcggc ttcgccgcgg catgatccgg
     1561 cggcatttgc cgcggatcca tttccaccac cgcctgcaac tgcccgccga ggctcgccac
     1621 cgtgtagatc cgacccttgt agccgacgag accggtgata tggccgtcgt gccaacgcat
     1681 gaggagggca ctttcgccag tctccgcgac gatcccgtgc caggtgcagc cacgctcgtc
     1741 gctcgagtaa cgcacgcgct caagggtgat gctcttggtg ggcgtaatgg gcagaacgac
     1801 ccgcgagggg gcctgctcct tcgacggcgt cgtctgcaat tccgccggga cgctcggccg
     1861 gtaggcgatc tcattggtca tcagaaaggc gctgaccgcg gcatccggcg cttgcaggac
     1921 gtgcacaccc atcgtgcccg gcgccgcctt cattctgtca accatggcct gctgggccgg
     1981 cgtcaccgga ccctcatggc gccgcagaat gtggttccag tcctcgcgca gcttcgtgac
     2041 cttcatgccg aggccctcga gccacttcga cacgcggctg gtctgccctt gcggcaccga
     2101 ccagacttcg gaattggccg cgcccgtgac ggcgccgttg ttcttgcaaa aaagcttctt
     2161 caacaagttg taaatcggcc ccttgcgctc cggcatattg gaaataatga ccatgtcggc
     2221 cggggcctgg ctgccctttt ccgggcgcgc cgcttcgctg cgccccgggc cgccctgcgc
     2281 tagcgccgtg ccggcgccgg gcaacagcag caaaaaaacc atcagcgccg cctggccggg
     2341 tcggccggcg cgccggtcag atctcatgct ggcgtacgtc acgaccgcac ccctgggctg
     2401 gtagcctaag cttgggcaaa atgggaattg cggccggtaa aatcaagcca gtaatccagc
     2461 caactcaagg ccttgttacc ggcagggggt gcctggttcg ccggtaagta gaaacggcgc
     2521 taggcgcgat cgcctggctt agaccttttg ctagtcacgg gccgccgacg gcaacctaac
     2581 cgcactgaac tggacaggcc ggccgctggc gcgggcacgc actgtcacgc agccgtcatc
     2641 gacgacttgc taactagcgg gtcgggaaag tgggacagtt caagggggag acggcacatg
     2701 gtcgatgcag ttggcgtcgc tggcgaagcg gcaaaacggc cgccgctcga ggttcagccc
     2761 aacttggtgg cggtgctgct gtttt
//
LOCUS       DASTMT010000028         1803 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_1651708, whole genome shotgun sequence.
ACCESSION   DASTMT010000028 DASTMT010000000
VERSION     DASTMT010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 1803)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1803)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1803
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1651708"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..611
                     /locus_tag="VFR00_00625"
     CDS             <1..611
                     /locus_tag="VFR00_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010968364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU0157779.1"
                     /translation="AQRPSWNYRAEDGGGIILDMLCHWRYLLDNTFGRVRAVSCLGAT
                     HIPERIDEAGRRYKASADDAAYATFQLADGVIAHFNSSWATRVRRDDLLTLHVDGTHG
                     SAVAGLQDVLCQHRMATPKPVWNPDVKQPINFFQGWQPVPDTQSFDNGFKLQWEAFIR
                     HVVEDAPYTWDLLEGAKGVQLVECALQSWKERRWIDVPELQV"
     gene            700..>1803
                     /locus_tag="VFR00_00630"
     CDS             700..>1803
                     /locus_tag="VFR00_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009490411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEU0157780.1"
                     /translation="MNAPTLSGMRITLPAAAGRLESFVLSEPRAYPDKAKGGFNRLAL
                     AAAHVVADATAANDPWLECALDWDRTIAYREYLWDLGLGVAEAMDTAQRGMGLDWPTS
                     LELIGRATRAAKAWEARNGRPAPLFCGCGTDHLDPQRVHTLDDVLAAYDEQMAAIEAV
                     GGRLIVMASRALARIAKSPADYERVYQRVLAQAGNPVIIHWLGEMFDPALAGYWGHSD
                     HRHAIESVLGVIAANAGKVDGIKISLLDKDKEIVMRRQLPAGVRMYTGDDFNYAELIA
                     GDAHGHSDALLGIFDAIAPAASAALGALAAGDRRQYEAIFEPTVPLSRHIFKAPTRFY
                     KTGVVFMAYLNGLQDHFVMVGGQQSARSLVHLSE"
BASE COUNT          313 a          581 c          590 g          319 t
ORIGIN      
        1 cggcgcagcg cccctcctgg aactaccgcg ccgaggatgg cggcggcatt attctcgata
       61 tgctttgtca ctggcgctat ctcctcgaca acacctttgg gcgcgtgcgg gccgtgtctt
      121 gccttggtgc cacgcacatc cccgagcgca tcgacgaagc cggccggcgc tacaaggcca
      181 gcgcggacga tgccgcctat gccacctttc agcttgccga tggggtgatt gcgcatttca
      241 acagctcgtg ggcgacgcgg gtgcgtcgcg atgatctttt gaccctgcac gtggacggca
      301 cgcacggctc ggcggtggcg ggcctgcagg acgtcctctg ccagcaccgg atggcaacgc
      361 cgaaacccgt ttggaatccc gatgtgaaac agccgattaa tttctttcaa ggctggcagc
      421 cagtgccgga cacgcaaagc ttcgacaatg gcttcaaatt gcaatgggaa gccttcatcc
      481 ggcacgttgt cgaggacgct ccttacactt gggacctgct cgaaggcgcc aagggcgtac
      541 agctcgtgga gtgtgccttg caaagctgga aggagcggcg ctggatcgac gttcccgagt
      601 tgcaggtgta agcgcgcgat cgcaaccgcc cgccgtgaga gcgcgaggat gcgtggcagg
      661 ctcgccgagg ccaacgcagg acagaacgga tagagagcca tgaacgcacc aacgctatcg
      721 gggatgcgca ttacgctgcc ggccgcggcg ggtcggctcg aaagctttgt gctctcggaa
      781 ccccgcgcct atccggacaa ggccaagggt gggttcaacc gcctcgcgct tgccgccgcc
      841 catgtcgtgg ccgacgcgac ggcggccaac gatccctggc tcgagtgcgc gctcgattgg
      901 gatcgcacga tcgcctaccg cgaatatctt tgggaccttg gcctcggcgt ggccgaggcc
      961 atggacaccg cccagcgcgg catgggcctt gactggccaa cctccctgga gctcatcggc
     1021 cgggccacgc gggcggccaa ggcctgggag gcgcgcaacg gccgaccggc tcccctcttc
     1081 tgcggctgcg gcacggatca cctcgatccc cagcgcgtgc acacgctcga cgatgtgctg
     1141 gcggcctacg acgagcagat ggccgcaatc gaagcagtcg gcggtcggct gatcgtgatg
     1201 gcgagccgcg cgctggcaag gattgccaag tcccccgccg attatgagcg cgtctatcag
     1261 cgcgtgctcg cgcaggcggg caaccccgta atcattcact ggctcggcga gatgttcgat
     1321 ccggcgctgg ccggctattg gggacacagc gatcatcgcc atgccatcga gagcgtcctt
     1381 ggcgtcatcg cggcgaacgc cggcaaggtc gatggcatca agatttctct gctggacaag
     1441 gataaggaga tcgtgatgcg ccgccaattg ccggccggcg tacgcatgta tacgggcgat
     1501 gacttcaact atgccgaatt gattgccggc gacgcgcatg gccacagcga tgcgttgctg
     1561 ggcattttcg acgccattgc cccggcggcc tcggcggccc tcggcgcctt ggcggcgggt
     1621 gatcgccggc agtacgaggc gattttcgag ccgaccgtcc ccctatcgcg gcacatcttc
     1681 aaggcgccga cccgcttcta caagaccggt gtcgtgttca tggcctacct gaacggcctg
     1741 caggaccatt tcgtgatggt cggcgggcag cagagcgcgc gctcgctggt ccatctcagc
     1801 gag
//
LOCUS       DASTMT010000029         2297 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_4928759, whole genome shotgun sequence.
ACCESSION   DASTMT010000029 DASTMT010000000
VERSION     DASTMT010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2297)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2297)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2297
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4928759"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..285)
                     /locus_tag="VFR00_00635"
     CDS             complement(<1..285)
                     /locus_tag="VFR00_00635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU0157781.1"
                     /translation="MHPVSRRQLIGTAAFAVALGLDKPLSWVTPAEAQTTPDPRLGFH
                     KYKVGAIEVAALYDGIWEKPHDPGFIKNASVEETQTALRQAGLPTEFVSIP"
     gene            510..1064
                     /locus_tag="VFR00_00640"
     CDS             510..1064
                     /locus_tag="VFR00_00640"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU0157782.1"
                     /translation="MSSRPSFEHLALQHLEAAYNLAFWLVRSRADAEDVVQEAYLRAL
                     RAFAGFRGDDMRPWLLAIVRHTAYRWLNERRRARNVVSIEEAYAAATAEGLVEPAATE
                     PNAEAQLIGAAEHDYVVRALADLPPAFREVLVLREIEGLSYRGIAEVTGVPIGTVMSR
                     LARGRRDLRKALLQLLAKDESNAR"
     gene            complement(1093..1965)
                     /locus_tag="VFR00_00645"
     CDS             complement(1093..1965)
                     /locus_tag="VFR00_00645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0157783.1"
                     /translation="MPVTPLASPSLTRAFGWLAVALAFALLWLALNVLLPVLQATGVA
                     SVAIRVTIHSLMLAGLWLGLARTSFATAARLKVWLALAVPITAWLAVVWWLALSGAFR
                     PRPGVPALPLAIFLPVLLAAPLLLNSKRIGTLLDATPTAWLVGLQVYRVFGGIFLVSW
                     SRGAIPGSFALPAGIGDVLVGVLALPVASLLASGHGEARRLALAWNVLGLLDFAIAIS
                     IGILSAPGPLQLIIPEPPNRELGTFPSVMIPAFAVPSSILLHVLSLRQLRRATASKPS
                     ASSRAGDVAPAPAR"
     gene            2092..>2297
                     /locus_tag="VFR00_00650"
     CDS             2092..>2297
                     /locus_tag="VFR00_00650"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU0157784.1"
                     /translation="MSSSYNLKCPIARTLDIIGERWTILILRDLFRDGPRKFQDLEQS
                     LAGASATTLSARLKRLEDHEIVARR"
BASE COUNT          412 a          744 c          788 g          353 t
ORIGIN      
        1 cggtatcgac acaaactcgg tcggcagccc ggcttggcgc aaggcggtct gggtctcctc
       61 gacactcgca tttttgatga agccggggtc gtgaggcttc tcccagatgc catcgtaaag
      121 cgcggccacc tcgatcgccc cgactttata tttgtggaac ccgaggcgcg ggtcgggggt
      181 cgtctgggcc tcggctggcg tgacccagga cagtggcttg tcaagtccga gcgcaacggc
      241 gaaggccgct gtgccgatca gctggcggcg tgagacgggg tgcacgcttt tcctcctttt
      301 tgcctggcaa cgcgggcggc ctctgcgggg cctcgatgag ctcagggccg atgccgtctc
      361 tagtccggga gggctcggtt tattccccag cgccaagcgc ggggctgggc gtggcactcg
      421 acggcgaccg gcgcgggaat aaacggcgtg tctcggggat agagaagagg ggtggcttcg
      481 tccctggcta aagcggcccg caggcggcca tgagttcgcg tccaagcttc gagcacctgg
      541 cgctgcagca tctcgaggcg gcgtacaatt tggcgttctg gctcgtgcgc tcacgtgccg
      601 acgccgagga cgtcgtacag gaggcctatt tgcgcgcctt gcgcgccttc gccggctttc
      661 gcggcgacga catgcggcct tggttgctcg ccatcgtgcg tcacaccgcc taccgctggc
      721 tcaacgagcg tcgacgggcc cgcaatgtgg tttcgatcga ggaggcctat gcggccgcca
      781 cggccgaggg gctggtcgag cccgccgcca cggaacccaa cgccgaagcg cagttgatcg
      841 gcgcggccga gcacgactat gtcgtgcgtg ccctggcgga cctaccgccg gcgttccgcg
      901 aagtcctggt gctgcgcgaa atcgaagggc tctcttaccg cggcattgcc gaggtcacag
      961 gcgtgccgat cggcaccgtc atgtcccgcc tcgcccgcgg tcggcgcgac ttgcgcaagg
     1021 cgctcctgca gctcttagcc aaggatgagt cgaacgcacg ctgagcggca ccacgacgcg
     1081 cgttcttcgc ttttagcgtg ccggcgcagg cgccacgtcg cccgcccggc tgctcgcgct
     1141 cggcttgctc gcggtggcgc ggcgcagctg ccgcagcgac agcacgtgca gcagaatgga
     1201 gctcggcacc gcaaaggctg ggatcatgac gctcggaaat gtcccgagct ccctattcgg
     1261 cggctcgggg atgatcagtt gcagcggccc gggtgccgac aggatgccga tggaaatggc
     1321 gatggcaaag tcgagcaggc ccaagacatt ccaggccagc gccaggcgtc gggcctcccc
     1381 atgaccgctg gccaagagcg aggcgacggg cagagccagg acgcccacta gcacgtcgcc
     1441 gatcccagcc ggtagggcaa agctgccggg gatcgcaccc cggctccagg acaccaggaa
     1501 gatgccgcca aacacgcggt agacctgaag cccgaccagc caggccgtcg gggtcgcgtc
     1561 gagcagcgtg ccgatgcgct tggagttgag aagcagtggg gcggcgagaa gaacgggaag
     1621 gaaaatggca agcggcagcg ccggcacgcc cgggcgtgga cggaaagctc cgctcagcgc
     1681 cagccaccac accacggcca gccaggcggt gatgggaacg gccagggcga gccaaacctt
     1741 gaggcgcgcc gcggttgcaa acgaggtgcg cgccaagccg agccagaggc cagccagcat
     1801 caggctgtgg atcgtcacgc ggatggcgac ggaggcaacg ccggtcgcct gtagcacggg
     1861 cagcaggacg ttcaaggcca accagagcag ggcgaacgcc aaggcaaccg ccagccagcc
     1921 gaacgcgcgt gtaagcgatg gggatgcaag cggggttacc ggcatggctc atctcctgcg
     1981 gacaaaggtc cattaaatgt attgactcta ttaattggag tcacttcacg aaatcgcgaa
     2041 aggctgctaa accctcgctc gccgcttgcc aggcgccgca gagaccgcag gatgtcatcg
     2101 agctacaatc tcaagtgtcc gatcgctcgc acgctcgaca tcatcggcga gcgctggacc
     2161 atcctgatct tgcgtgacct ctttcgcgac ggcccgcgca agttccagga cctcgaacag
     2221 tccttggcgg gggccagcgc caccacgctc tcggcgcggc tcaagcggct cgaggaccac
     2281 gagatcgtgg cgcgccg
//
LOCUS       DASTMT010000030         6625 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_2309683, whole genome shotgun sequence.
ACCESSION   DASTMT010000030 DASTMT010000000
VERSION     DASTMT010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 6625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6625)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6625
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2309683"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..596
                     /locus_tag="VFR00_00655"
     CDS             <1..596
                     /locus_tag="VFR00_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008132758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEU0157785.1"
                     /translation="AALARGEGAVGTATPLPPAGIERARAYFADAAGTAARYLARPDG
                     PRIGAMGFVGWDTHVNEGAADGQLAGLLGALDGALTAMETNLGSAWQETVVVVTTEFG
                     RTARINGSNGTDHGTGTVALLAGGALKGGRVLADWPGLKSADLYEGRDLKPTIDLRAV
                     LKGLLRDHLRVEEKALAASIFPDSSDVAATPGLLRSA"
     gene            759..1949
                     /locus_tag="VFR00_00660"
     CDS             759..1949
                     /locus_tag="VFR00_00660"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR04350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004121 - cystathionine beta-lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HEU0157786.1"
                     /translation="MFDFDRVIERRGTHASKWDNMAKLSGITAADAIPMWVADMDFAA
                     PPGVSEALLAETRRGVHGYYADTGSWAGALVAWLRRRHDFRVEPAWISPTPGIVAGLG
                     LILQALSAPGDEVVVFPPAYHAFRRIILANERRILDAPLIERDGRYQMALEALRARLT
                     PRSKVVFFCSPHNPGGTVWSVDEIRALADFCAEHDLILVSDEIHADLVFAGAKHTPTM
                     LAAPHIAERLITCVAATKTFNLAGAHVGACITSNAEMKRRLDARVMASGLGSYNSFGM
                     LATEAAWRTGDQWLDALLRYLTQSRDAFDRRIEAAVPGARSMRLDATYLAWVDFAGTG
                     LSPEDVAGRVKGRARIFASPGPQFGPGGETWLRFNFATPRPVLEEALGRLEAAFGDFR
                     ASRT"
     gene            1960..2730
                     /locus_tag="VFR00_00665"
     CDS             1960..2730
                     /locus_tag="VFR00_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011512319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEU0157787.1"
                     /translation="MKRPVVGVIGNAHRVENRFNIHGVGERNLRAVAEVAGALPLMFA
                     GTPHVTDVGALLQAVDGILLTGARANVHPSRFRKEAHPAHEPYDEDRDALALALVEAC
                     VDKGIPIFGVCRGFQEMNVAYGGSLHPEIRDLPGRMNHRMPRLETGEIHPDPAVIFAD
                     RHDVSLIPGGTFAQILGCECIRVNSLHGQGILEPGGRIVVEGVAEDGTIEAIRVAEAP
                     GFALGVQWHAEHNAQGNTVNRALFQAFGEALLARQSRR"
     gene            complement(2898..4229)
                     /locus_tag="VFR00_00670"
     CDS             complement(2898..4229)
                     /locus_tag="VFR00_00670"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014494485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEU0157788.1"
                     /translation="MSRRITRVLLGAAMVLFATSASLGLFPSLATAQRSKAAATPIAS
                     GTPESVGMSTARLARLTDAFKKEIGEKRLPGVVMMVSRRGRLVYSTALGLRDPKGEDP
                     MRTNSLFRIYSMTKPIVSVGAMLLVEDGTLLLNDPVSKWLPAFKDVKVWTANGEVSAE
                     RPMTVQDLLRHTAGLPYGELTQNATVKDALAKAGLFKPGVIEFDARDMTGAEQVERLA
                     KIPLLYQPGTVWEYSLASDLLGRVVEAASGKRLGDFLSQRIFKPLKMVDTAFWVSEAN
                     RGRLAEPFEKDAVAGTPVKLIDVSTQPGNDSGGAGGISTAGDYLRFAQMLANGGTLDG
                     QRIMSRTTLRLMTSDHLAGRLPLAATPGGMVLGASTYTFGLGFAVRPGDGLAPFPGSA
                     GDFNWGGYAGTFFWVDPKEQLVATLMVQSAGTLRLYHRLLYRQLVYQAILD"
     gene            4488..4649
                     /locus_tag="VFR00_00675"
     CDS             4488..4649
                     /locus_tag="VFR00_00675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0157789.1"
                     /translation="MKHLIVAAMIAAFGAAVLLPATAVISSEPAFAAQKKAKKTAKKP
                     AKKKPTQSM"
     gene            5128..5706
                     /locus_tag="VFR00_00680"
     CDS             5128..5706
                     /locus_tag="VFR00_00680"
                     /EC_number="2.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019400536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0018104 - peptidoglycan-protein
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEU0157790.1"
                     /translation="MTKPLFGPARGLRLAFACLCAVSGLAGPAAAQGFLSFLWSGGSR
                     EVVAFHPSHAPGQIIVSFGDRRLYWVHRRGEAISYPIAVPREKSRWQGVTSVSDKRVN
                     PDWTPTAEMRRENPRLPPHVPGGHPRNPLGVRALYLGSSMYRIHGTDAPWTIGDAVSK
                     GCIRLYNEDVLDLYRRVPVGTRVTVTWRRFNA"
     gene            6297..>6625
                     /locus_tag="VFR00_00685"
     CDS             6297..>6625
                     /locus_tag="VFR00_00685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0157791.1"
                     /translation="MATPAAADGGGGPGPYYPSIWQGFYGGVQAGFGWAGDADGGFAG
                     GQVGYNWRSQQFVYGLEADIAAADIGVSGSATACQQGFGCATVQASGSIDWFATIRGR
                     AGILVQPG"
BASE COUNT         1065 a         2161 c         2237 g         1162 t
ORIGIN      
        1 tggcggcgct ggcgcgtggt gagggcgccg ttggcaccgc cacccccttg cccccggcag
       61 ggatcgaacg ggcgcgcgcc tacttcgccg acgccgctgg cacggccgcg cgctacctgg
      121 cccggccgga tgggccgcgc atcggcgcga tgggcttcgt cggttgggat acgcacgtca
      181 acgagggcgc cgccgacggc cagctcgccg gtctgctggg cgcccttgac ggtgcgctga
      241 cggcgatgga gaccaacttg ggctcggcct ggcaggagac cgtggtcgtg gtgaccacgg
      301 aattcggccg caccgcccgc atcaacggca gcaatggtac cgatcacggc accggcacgg
      361 ttgccctgct ggcaggcggg gcgctcaagg gggggcgcgt gctggcggat tggcccgggc
      421 tcaagagcgc cgatctctac gaggggcgcg atctgaaacc gacgatcgat ctgcgcgccg
      481 ttttgaaggg cctgctgcgg gaccacttgc gggtcgagga gaaagcgctg gcggccagca
      541 ttttcccgga cagcagcgac gttgcggcca cgccggggct gttgcgcagc gcctaggcgc
      601 cgaccgggcc ggcggccgat cggctttggc ccaccctccc tgggcgagca gcaggggcgc
      661 gtttgaccac cttgacccgc tcctgagcac cccgtttcaa tgggcggctg tgaccaaagg
      721 gaatcgcccc gcggcaggtg caggagctaa gagccggcat gttcgacttc gaccgcgtca
      781 tcgagcgccg cggcacgcat gcgtccaaat gggacaacat ggcgaagctt tcgggcatta
      841 cggcagccga tgcgatcccc atgtgggtgg cggacatgga cttcgcggcg ccgcccggcg
      901 tcagcgaggc cttgctggcc gagacacgcc gcggcgtgca cggctactat gccgatacgg
      961 gaagctgggc cggcgcactc gtggcctggc tgcgtcggcg ccacgatttc cgcgtggagc
     1021 cggcctggat tagccccacc ccggggatcg ttgcgggctt ggggcttatc ctgcaggcac
     1081 tgagcgcgcc gggcgacgag gtcgtggttt ttccacccgc ctaccatgcc tttcgccgga
     1141 tcattctcgc caacgagcga cgcatcctcg atgccccgct gatcgagcgc gacggccgct
     1201 atcagatggc gctcgaggct ttgcgtgcga ggctcacacc gcgcagcaag gtggtgtttt
     1261 tctgcagccc ccataatccc ggtggcacgg tctggagtgt cgacgaaatc cgcgcgctgg
     1321 ccgatttctg cgccgagcac gatctgatcc tggtctctga cgagatccac gccgatctgg
     1381 tctttgcggg ggcgaagcac acccccacca tgctggcggc gccgcacatt gccgagcggc
     1441 tgatcacctg tgtcgccgca accaagacct tcaaccttgc cggcgcccac gtcggcgcct
     1501 gcatcacctc caatgccgag atgaaacggc gcttggatgc gcgcgtgatg gcaagtggcc
     1561 tcggctccta caacagcttt ggcatgctcg cgacggaggc ggcttggcgc accggcgatc
     1621 agtggctcga cgcgcttttg cgctacctga cgcaaagccg cgacgcgttc gatcgccgca
     1681 tcgaggccgc ggttcccggc gcccgctcga tgcgtctcga cgccacctat cttgcctggg
     1741 tcgattttgc cggcaccggc ttgtcgcccg aggacgtcgc cggccgcgtc aagggccggg
     1801 cgcgtatttt cgccagtccc ggtccccagt tcgggcccgg cggggagacc tggctgcgct
     1861 tcaactttgc gacgccgcgg ccggtcctcg aggaggcgtt gggccgcctc gaggcggcat
     1921 tcggcgactt tcgcgcgtcg cgcacgtgag agctaggcga tgaagagacc agtcgtcggg
     1981 gtgattggaa acgcgcaccg ggtcgagaac cgcttcaata ttcacggggt tggcgagcga
     2041 aacctgcgcg cggtggcaga agttgccggc gcgctgccct tgatgtttgc gggcacgcct
     2101 cacgtcaccg atgtcggcgc gctgctgcag gccgtcgatg gcatcttgct gacgggagcg
     2161 cgggcgaacg tgcatccgtc gcgcttccgc aaggaagccc accctgcgca cgagccctac
     2221 gatgaagacc gcgatgcgct ggcgctggcc ttggtcgagg cctgtgtgga caagggcatc
     2281 ccgatcttcg gggtctgtcg tggctttcag gagatgaatg tcgcctatgg ggggagcctg
     2341 cacccggaaa tccgcgactt gccggggcgt atgaaccatc gcatgccgcg cctggaaacg
     2401 ggggaaatcc atcccgatcc cgccgtcatt ttcgccgatc ggcacgacgt cagtctcatt
     2461 cccggcggca cgttcgcgca aatcctgggc tgcgagtgca tccgcgtcaa ttccctgcac
     2521 gggcagggta tcctcgagcc gggggggcgc attgtcgtgg aaggcgtggc cgaggacggt
     2581 accatcgagg cgatccgggt ggccgaagcc ccgggtttcg cgctgggtgt gcaatggcac
     2641 gccgagcaca acgcgcaggg caacaccgtc aatcgtgccc tcttccaggc ctttggcgag
     2701 gccctattgg cccgccaaag ccggcgttag ccagcactct tccccatcgc ggctaagtgt
     2761 cgaaggtccg aggctcggca gccaacgggg gctgccggcg cgcacgagga ggcggcgccg
     2821 cgggcagcca cgccgctcgg ccagccgccg ccggcgcggt tgccgtctcg aggtgtcgcg
     2881 tgtcggccgg gcgatcctca gtcgaggatc gcttgataga cgagctgccg gtaaaggagc
     2941 cggtggtaga ggcgcagggt gcccgccgat tgcaccatca gggtcgccac cagctgctcc
     3001 ttgggatcga cccagaaaaa cgtgccggca tagccgcccc agttgaagtc tccggccgat
     3061 cccgggaagg gtgcaagccc gtcgcccggc cgcacggcaa agccgaggcc aaacgtgtag
     3121 gtcgaggcgc cgagcaccat gcctcccggc gtcgcagcca gcggcagccg cccggccaga
     3181 tgatcggagg tcatcagccg cagcgtggtg cggctcataa tgcgctgtcc gtcgagcgtg
     3241 ccgccattgg ccaacatctg ggcaaagcgc aggtagtcgc cggctgtcga aatcccgccg
     3301 gcaccgcctg aatcgttgcc gggttgcgtc gagacgtcga tgagcttgac cggcgtaccg
     3361 gcgacggcgt ctttttcgaa gggctccgcc agacggccgc ggttggcttc ggagacccag
     3421 aaggccgtgt cgaccatctt gagcggcttg aagatacgct ggctcaggaa gtcgccgagc
     3481 cgcttgccag atgccgcctc cacgacgcgg ccgaggaggt cggaggcgag gctgtattcc
     3541 caaaccgttc ccggttggta aagcaggggg atcttggcga ggcgctcgac ctgctcggcg
     3601 ccggtcatgt cgcgtgcgtc gaactcgatg acgcctggct tgaacagccc cgccttggcg
     3661 agggcatcct taacggtggc gttctgggtc agctcgccat agggcaggcc ggcggtgtgc
     3721 cgcagcaaat cctgcacggt catcggccgc tcagctgaga cttcaccgtt ggccgtccac
     3781 accttcacgt ccttgaaggc gggtaaccac ttggagaccg ggtcattcag caacagcgtg
     3841 ccgtcttcga caaggagcat ggccccaacc gagacgatgg gcttggtcat cgaatagatg
     3901 cggaacagcg aattggtccg catcggatcc tcgcctttgg gatcgcgaag gcccaacgcg
     3961 gtcgagtaga cgagccggcc acgtcgcgac accatcatga cgaccccggg cagccgcttc
     4021 tcgccgatct cctttttgaa ggcgtcggtc agtcgggcca ggcgggccgt cgacatcccc
     4081 acgctttccg gcgtgccgct cgcgatcggc gtcgcggcgg ctttgctgcg ctgcgccgtg
     4141 gcgagcgagg gaaagagtcc caggctggcg ctggtcgcga acaagaccat tgccgcgccc
     4201 agcaggacgc gtgttattcg gcgagacatg gcagttcccc ccttttgtta acgttatgcg
     4261 gtttgagctc gtcgaggtta gcacgtaggc cgggcaaaat tgcgttgcct tggacagcgg
     4321 gcgctgcatg gcagccgtgc gcatgcaacg gcaaggctag cccctttttg gcaggctccg
     4381 ccccgtcaac aaagacccgc ccctttgtca ggaattctcc tgattgattt gtaagcggag
     4441 ctaattgagc gtctaaggcc cacgaggctt cgcaatggag acattctatg aagcacttga
     4501 ttgtagccgc catgatcgcc gccttcggcg cggccgtgct gctgcccgcc actgccgtga
     4561 tcagctcgga gccggcgttt gcagcacaaa agaaggccaa gaagaccgcc aaaaagccgg
     4621 cgaagaaaaa gccgacgcaa tccatgtaaa gggtcgtcta ggaggccacg cccggcgtct
     4681 cgccccggtg gccgctacgc ctcttcgttg cggcgaacct ccgccttgct gcagcacgcc
     4741 ttgctgaagc acgggggttc gcccgcttgc tttgcgcgct ccttccagcg ttttgccacg
     4801 tccgcacttt tcctctccgc gtcagcctcg atcgagcggg gcagggaccc cgagcgcgat
     4861 cttcgaacca atagacgacg gtcattgatg ccgttggctg cgtcggcgca acgcggctcc
     4921 cgacactgca ctcggccctc cacaggcagc tcggcttgac gcacgaccgt gccatgcgcg
     4981 tcggcggccg cagcaggctg cctgcgcgag gttagccggc gcagaaagcg tttttgtgcg
     5041 cggatgtgtc tccgcgaggc taggctgcca aaagcatcgc gcggagaatc accgcgaaac
     5101 tgattcgttt ctcgaccgag accagcgatg acaaaaccgc tcttcgggcc tgcgcgcggg
     5161 ctgcgcctgg cattcgcctg cctttgtgcc gtctcgggtc tcgccgggcc ggctgcggcg
     5221 cagggctttt tatcgttcct gtggagtgga ggctcacgcg aggttgtggc cttccatccc
     5281 tcccatgccc cgggccaaat catcgtcagt ttcggggacc ggcggctcta ttgggtgcac
     5341 cggcgcggcg aggccatcag ctatcccatc gccgttccgc gggagaaaag ccgctggcag
     5401 ggcgtgacca gcgtgtccga caagcgggtc aatccggact ggacgccaac ggcggaaatg
     5461 cgacgtgaaa atccgcgcct gccgccgcac gtgcccggcg ggcacccgcg caatcccctg
     5521 ggcgtgcgcg cgctctatct gggatccagc atgtaccgca ttcacggcac cgatgcgccc
     5581 tggaccatcg gcgatgcggt ctccaagggc tgcattcggc tctacaacga agacgtgctc
     5641 gatctttatc ggcgcgttcc cgtcggcacg cgcgtgaccg tcacctggcg acgctttaat
     5701 gcttgaaacg cgcctcgttt ctgaacgatc gccctaacgt cggcgaacgg cgccggcgtg
     5761 ctgacggaga gaaaacacgt ccgggcgcat ctctctcacc ccgaagcggt gtcccatctc
     5821 ggctaggttg ggtgccagcg gcattccggc gcccgatgct cgccgtggcg ccttgccgcc
     5881 atttgccgtg atcggctagg tgagcgcggg ccgctcggga tggtggtccc gaatcaggtg
     5941 cggcctcggc acccctacac gggacgccga tgcgcgctcg cggccctgca tttccgccgc
     6001 gcgtggtcgc ttcgccacag aaatactcga tttcgtggca agtcccggtg cggtctaacg
     6061 caatgaagaa tttcgcaaat tccaaatggg gcgcgcaccc gacaccgcca gaggttaccc
     6121 acacggtggc tctgggcatc tgaacggtgt cacacaatgc ggctatggtc cgccgcttag
     6181 cgcggaggaa tccgcatcgg gtgcggggaa cctgatcatt tgaccgggga gcactcgagc
     6241 atgaaggcat tacttggggg cgcattggca ttgggtgcct agcaggcgtg gccgcgatgg
     6301 cgacgccggc ggccgccgac ggaggcggcg gtccggggcc ctactatcca tcgatctggc
     6361 aaggcttcta cggtggcgtg caagccggtt ttggctgggc gggcgatgcc gatggcggct
     6421 tcgctggcgg ccaggtcggc tacaactggc gatcgcagca gtttgtgtat ggcctcgagg
     6481 ccgacatcgc ggccgccgac atcggtgtca gcggcagcgc caccgcctgc cagcagggat
     6541 ttggttgcgc tacggtgcag gcgagcggat cgatcgactg gttcgccacg attcgtggtc
     6601 gtgctggaat tctggtgcaa ccggg
//
LOCUS       DASTMT010000031         1819 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_6241827, whole genome shotgun sequence.
ACCESSION   DASTMT010000031 DASTMT010000000
VERSION     DASTMT010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 1819)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1819)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1819
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6241827"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..591
                     /locus_tag="VFR00_00690"
     CDS             <1..591
                     /locus_tag="VFR00_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin-like C-terminal cupin domain-containing
                     protein"
                     /protein_id="HEU0157792.1"
                     /translation="LVTGGQGLAHVKGVAPNATTRRLFGIQAWAALPQSHEEGPPGFA
                     HHGTAELPRISGEGKTVRLVLGAAYGSRSPVAFPHESLFAEAVLAPGAVLPLDPDHDE
                     RAVYVASGEIDIAGSRFAEGRLLIFRPGDRISILALAHSRLVLVGGEPMDGPRHIWWN
                     FVSSSKERIEQAKADWRAKRFALVPGDEKEFIPLPE"
     gene            complement(637..1500)
                     /gene="sseA"
                     /locus_tag="VFR00_00695"
     CDS             complement(637..1500)
                     /gene="sseA"
                     /locus_tag="VFR00_00695"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013420516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HEU0157793.1"
                     /translation="MANGEHKWLVETDWLASHLEAPDLVILDGSMHLPTTKRNAKAEF
                     LAAHIPGAMFFDIDDIADETSALPHMLPSPTKFASRMKKMGVGDGMHVVAYDSEGLYS
                     AARVWWMFRTMGHEEVRVLNGGLKKWKAEGRPLEDGAPRQRTPRHFTPVFNASLVRDA
                     AEVKALIDSPVAQIVDARAAGRFEGSVTEPRAGLRSGHIPSARNLPFASLVNADGTLK
                     AAPELRQIFAAAKVDADRPVVASCGSGVTAGVIALALAVLGRPDAAVYDGSWSEWGAD
                     PSLPIATGPAR"
     gene            complement(1630..>1819)
                     /locus_tag="VFR00_00700"
     CDS             complement(1630..>1819)
                     /locus_tag="VFR00_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018698292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alanyl-tRNA editing protein"
                     /protein_id="HEU0157794.1"
                     /translation="ISVKPPMRSGKVRLVVIGENGSVDLQPCGGTHVRSTGEIGAVVV
                     SKMEKKGKLNRRIRVAFA"
BASE COUNT          301 a          598 c          593 g          327 t
ORIGIN      
        1 ctcgtcacgg gcgggcaggg gctggcgcac gtcaagggtg tggcccccaa cgcgactacc
       61 cggcgcctgt tcggcattca agcctgggcg gccctgccgc agagccatga ggaggggccg
      121 cccggcttcg cccatcacgg caccgccgaa ctgccgcgca tcagcggtga aggcaagacg
      181 gtgcggttgg tattgggcgc ggcctatggg tcacggtcgc ccgtggcatt tcctcacgag
      241 tcgctgtttg ccgaagccgt gctggcacca ggagccgtgc ttccgctcga tcctgatcac
      301 gacgagcggg cggtctatgt cgcctccggt gaaatcgata tcgccgggag ccgcttcgct
      361 gaggggagat tgctgatctt caggccgggc gaccgcatct cgatcttggc ccttgcgcac
      421 tctcggctgg tgctggtggg cggagagcct atggatggcc cccgccatat ctggtggaac
      481 ttcgtatcct cgtcgaaaga acggatcgaa caggccaagg ccgattggcg cgccaaacgg
      541 tttgccctgg tgccgggcga cgaaaaagag ttcatcccgc tgccagagtg aagagctcgg
      601 tacgcctctc ggcaagggct tgcctgccag ccgccgctat cgggccggac cagtcgcaat
      661 cggcagcgac ggatcggcgc cccattcact ccaggagccg tcgtagacgg cggcgtccgg
      721 cctgcccagc acggcaaggg cgagggcaat gacccccgcc gtaaccccgg agccgcacga
      781 ggcaaccacc ggccgatcgg cgtcgacttt ggcggcggca aaaatttgcc tgagttcggg
      841 tgccgctttt aaggttccgt ccgcattgac gaggctcgca aacggcaggt tgcgcgcgct
      901 gggtatgtga cccgagcgta ggccggcgcg cggctccgta acgctgccct cgaagcgtcc
      961 ggccgctcgg gcatccacga tctgcgccac agggctgtcg atcagggcct tcacttcggc
     1021 ggcatcgcgc accaacgagg cattgaagac cggtgtaaag tggcgcggcg tgcgctggcg
     1081 cggtgcgcca tcctccagcg gccgaccttc ggccttccat ttcttgaggc cgccgttcaa
     1141 gacgcgcacc tcctcgtgac ccatggtgcg aaacatccac cacacgcgcg ccgccgagta
     1201 gagcccctcg ctgtcatagg caacgacgtg catgccatcg ccgacgccca tctttttcat
     1261 gcggctggcg aacttggtcg gggaaggcag catgtggggc aaggccgagg tttcgtcggc
     1321 aatgtcgtca atgtcaaaaa acatggcgcc gggaatatgg gccgcaagaa actcggcttt
     1381 ggcattgcgc ttggtggtcg gcaaatgcat cgacccatcg agaatgacga ggtcgggtgc
     1441 ctccaggtgt gacgccagcc agtccgtctc gaccagccat ttgtgctcac cgttggccat
     1501 acccgtttcc ttcctgctcg cggtcttcac gtcctggcgc gcgccgctct ggacagggtc
     1561 ctcgcggccg gctgcgctgc gtgacggcgc gacccggcac gccgaccgaa cgcaggcgcg
     1621 tctttgctgt catgcgaaag cgacgcggat gcgccggttc aatttgccct tcttttccat
     1681 cttactgaca acgacggcgc caatctcgcc ggtgctgcgc acgtgcgtgc cgccgcatgg
     1741 ctgcaaatcg accgaaccgt tttccccgat cacgacgagg cggaccttgc cgctgcgcat
     1801 ggggggcttc accgagatg
//
LOCUS       DASTMT010000032         4519 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_1982328, whole genome shotgun sequence.
ACCESSION   DASTMT010000032 DASTMT010000000
VERSION     DASTMT010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4519)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4519)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4519
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1982328"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            22..1683
                     /gene="ettA"
                     /locus_tag="VFR00_00705"
     CDS             22..1683
                     /gene="ettA"
                     /locus_tag="VFR00_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HEU0157795.1"
                     /translation="MANPQYVYHMHKLSKTYPGGKQVLKDISLSFFPGAKIGVVGLNG
                     AGKSTLLKIMAGDLEEYIGEAWAADGVRVGYLAQEPELDPAKDVLGNAMEGVAEKKAL
                     VDRYNELAANYSEETAEEMARLQDEIEAQDLWDLDTQVEQALDALRCPPGDQDVATLS
                     GGEKRRVALTRLLLAKPEILLLDEPTNHLDAESVAWLERYLKDYAGCIMLVTHDRYFL
                     DNITEWTLELDRGHGVPYKGNYSSWLEQKAKRLEVEKGQEEAKQRTLKRELEWIRSSP
                     KARQAKSKARIQAYERLAEEAGREQTGKAQIQIPPGPRLGGVVLEVEGLSKAFGDRLL
                     IDELSFQLPPGGIVGVIGPNGAGKTTLFKMLTGAEKPDSGSIRLGDSVKLGYVDQSRD
                     HLDAKKSVWEEVSNGLDIMKLGDKMEVNSRAYVSWFNFKGPDQQKKVGLLSGGERNRV
                     HLAKMLKDGGNLLLLDEPTNDLDTETLSALEAALEDFAGCAVIISHDRFFLDRMATHI
                     LSFEGDSHVEWFEGNFQDYEADKKRRLGEAAVEPHRIKFKRFERR"
     gene            complement(1848..2483)
                     /locus_tag="VFR00_00710"
     CDS             complement(1848..2483)
                     /locus_tag="VFR00_00710"
                     /inference="COORDINATES: protein motif:HMM:NF017319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0157796.1"
                     /translation="MIKVLSCIASLARGSRAGLAAGLCVTLLLLASFVADRLGVILPA
                     GAADPPVIYMSQVGKELMSAARTRSPGVMATVIQRHADVSYIGLYSLGNYRAKLAATD
                     RDTYFSGMVRFISRYAASEAPKYPVSRVNWTNQSTRGSSGVMVDSQVVMQDGTAYDVR
                     WLLAKYGNGYKVRDAMVLGFWMTPFLKKLFEDYIEQNGGNLHALTAALNRS"
     gene            complement(2730..3212)
                     /locus_tag="VFR00_00715"
     CDS             complement(2730..3212)
                     /locus_tag="VFR00_00715"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HEU0157797.1"
                     /translation="MTTLTRLRLIGAAAIVSSAAALSAAAQTQTPPPETQPSTAPAAK
                     PEPAPTPPPTGMKPTEPPKSPTMPSTDKQSAAPAKNPLIGLAVFSSDGSKLGSVQSID
                     AGPDGKVKAIRFKTGGFLGFGGKLVEIPEGKFTKSGDNVQLGMTSDEVSKLPEVKNQS
                     "
     gene            3746..>4519
                     /locus_tag="VFR00_00720"
     CDS             3746..>4519
                     /locus_tag="VFR00_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018387014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEU0157798.1"
                     /translation="MAALSQQMCVNVLKAMAEPTRLRVLALLAYGELNVKDLTRVLGQ
                     SQPRISRHLKLLCEAGLVERAPEGSWVYFRLSEQPMGSGLPHLVLSEVDANDPVLLRD
                     RARAEALRAEREAIAQAYFQAHAKEWDTIRALHVAEASVEAAIAAALEGEPLGFLIDL
                     GTGTGRMLELLAARYRRGLGIDRSPAMLAYARAKLERAKLAHAQVRQGDIYDLPLGDG
                     LADVVLMHQVMHFLGDPPQAIREAARVLRPGGRLLIVDFA"
BASE COUNT          815 a         1441 c         1506 g          757 t
ORIGIN      
        1 gatccgaacg tcgaggcgcc cgtggcgaac ccccaatacg tctaccacat gcacaagctg
       61 tccaagacct accctggggg caagcaggtc ttgaaggaca tctcgctgtc gttcttcccc
      121 ggcgccaaga tcggcgtcgt cggcttgaac ggtgcgggca agtcgacctt gctcaagatc
      181 atggccggcg acctcgagga gtatatcggg gaggcctggg ccgcggacgg cgttcgggtt
      241 ggatacttgg cgcaggagcc cgagctcgat cccgccaagg acgtgctcgg caacgccatg
      301 gaaggggtgg cggagaagaa agcgctggtc gatcgctaca acgagctcgc cgccaactat
      361 tccgaggaga ccgccgagga gatggcgcgg ctgcaggatg aaatcgaagc ccaggacctg
      421 tgggatctcg acacccaggt ggagcaggcg ctcgatgcct tgcgctgccc gccgggagac
      481 caggacgtcg ccacgctttc gggtggcgaa aagcggcgcg tggccctgac ccggcttctg
      541 ctcgccaagc ccgagatcct cctgctcgac gagcccacca atcacctcga cgcggaatcg
      601 gtggcctggc tggaaagata tctcaaggac tacgccggct gcatcatgct ggtgacgcac
      661 gaccgctact tcctcgacaa catcacggag tggacgctcg agctcgatcg cgggcacggc
      721 gtgccctaca agggcaacta ttcgagctgg ctggagcaaa aggccaagcg cctcgaggtg
      781 gagaaggggc aggaggaagc aaagcagcgc acgctgaagc gcgaactgga atggatcaga
      841 tcctcgccca aggcgcgcca ggcgaagtcg aaggcacgca ttcaggccta cgagcgcttg
      901 gccgaggaag ccggtcgcga gcagaccggc aaggcgcaga ttcaaattcc gcccggcccg
      961 cggcttggcg gcgtggtgct cgaggtcgaa ggcttgtcga aggccttcgg cgaccgacta
     1021 ttgatcgatg agctctcctt ccagctgccg cccggcggca tcgttggcgt gatcggcccg
     1081 aacggggcag gcaagaccac gctcttcaag atgctcaccg gcgcagagaa gccggatagc
     1141 ggcagcatcc ggcttggcga cagcgtcaag ctcggctacg tcgaccaaag ccgcgaccat
     1201 ctcgacgcca agaagtccgt ttgggaggaa gtctcgaacg gtctcgacat catgaaactc
     1261 ggcgacaaga tggaagtgaa cagccgcgcc tacgtgagct ggttcaactt caaaggcccc
     1321 gatcagcaaa agaaggtcgg cctgctttcg ggcggcgagc gcaatcgcgt gcacctggca
     1381 aagatgctga aggatggcgg caatctcttg ttgctcgacg agcccaccaa cgacctcgat
     1441 acggagacct tatcggccct ggaggcggcg ctggaagact tcgccggctg cgccgtcatc
     1501 atcagccacg atcgcttttt cctcgaccgt atggccaccc acatcctctc cttcgagggg
     1561 gatagccatg tcgagtggtt cgaaggcaat tttcaggatt acgaggccga caaaaagcgc
     1621 cgcctcggcg aagccgccgt ggagccgcac cgcatcaagt tcaagcgctt cgagcggcgc
     1681 tagcccgccg cgtcgggcgg gatggtgcga cggcggcgcc gcagcgaaga tggcgcctgc
     1741 cacgaccaag acgctgcgcc aggggagagg ttgcgcgcct cgctctacga cctggagccg
     1801 ctgctgagga gacgtcgcgc accccagcgt caaccaattg ccggcgccta gctgcgattg
     1861 agcgcggcgg tcagggcgtg gagattgccg ccgttctgtt cgatgtagtc ttcgaacagc
     1921 ttcttgagga acggcgtcat ccaaaaaccc aataccatgg cgtcgcggac cttgtagccg
     1981 ttgccgtact tggcgagcag ccagcgcacg tcgtaggccg tgccgtcctg catcaccact
     2041 tggctgtcga ccatgacccc gctgctgccc cgcgtgctct ggttggtcca gttgacgcgt
     2101 gagacggggt atttgggggc ttcgctggcg gcatagcggg aaataaagcg aaccatgccg
     2161 gagaagtagg tgtcccggtc ggtcgcggcc agcttggcgc gataattgcc gagcgaataa
     2221 agcccgatgt aggagacatc ggcgtggcgc tggatcacgg ttgccatcac gccgggggag
     2281 cgtgtgcggg ccgccgacat gagctccttg ccgacctggg acatgtagat cacgggcgga
     2341 tcggcggcgc cggccggcaa gatgacgccc aagcgatcgg cgacgaacga ggcaagcagc
     2401 aaaagggtga cgcagagccc tgcggcaagg cccgcccgag agcccctggc aaggctggcg
     2461 atgcaactca ataccttgat catgcgaaac gtttcccctc tcccatgacg cagcccccgc
     2521 ggccgtcatc tggagcgtgc acgtgttttg aacacggaat ggggcgcccc gtcaaaggcc
     2581 tcccgccgcc gatgatcgga tcgggtgtgc ccctccatgt cactcaattg tgtcgagccg
     2641 gcgatccgca acgccgcctt tgacgcggga agcaaaaaac gggccgcgcc cgaaggcgcg
     2701 gcccgtcgca acagccaaca aagagaccgt tagctctgat tcttcacttc cggcagcttg
     2761 ctgacttcat cagaggtcat gccgagctgc acattatcgc cggatttggt gaatttgccc
     2821 tcggggatct ccacgagctt gccgccaaat ccgagaaagc cgcccgtttt gaaacggatg
     2881 gccttgacct tgccgtccgg gcccgcgtcg atgctctgca ccgatcccaa cttgctgccg
     2941 tcggaggaaa agaccgcgag gccgatcaag ggattcttgg ccggcgccgc cgattgcttg
     3001 tcggtcgacg gcatcgtcgg actcttcggc ggctcggtgg gtttcatccc cgtcggcggc
     3061 ggcgtcggcg ccggctccgg cttggccgcg ggagcggtgc tgggctgggt ctccgggggc
     3121 ggcgtttggg tctgggccgc ggcagagagc gccgcggcgc tcgagacgat ggcggcggca
     3181 ccgatcaggc gcaggcgcgt aagcgtcgtc atgagttcat ctccgtttgt tgttgctgct
     3241 ccggggacaa cccaccaccc gcgcaaaggt tgcgcctgga ccccgcgggg aacggggctt
     3301 tttagggccg cgcgcgggcc aggcggcggg acattttgtt gccatctttc ggccagcggg
     3361 gcgccgcaaa tagtgcgcgt cggaagccct cgcagagcaa gggatccgcg gcccgggagg
     3421 tgccgcgcaa gcggatttag agcgtgtact gagcaacctc tttggcgctg acgcgtaacg
     3481 cagaacacgc tggccctgcc cagatctgac cccgtcgcct cgcggcggtg cgccggccgc
     3541 cagggttggc tggcaggcac ccgtctatgg gccctcccat cggtgcccct ttgtggcgcc
     3601 aagcgcccgc tccgagcggg gactggccgt gccgccggcg gcgtcgcagg ccgctcggca
     3661 cgctcctgtg ggccggccca acgctcggtc ttgacacata aggatatctt tatatggttg
     3721 tgatgcgcga ggctgaagga ccgccatggc tgccttgtcc caacagatgt gcgtcaatgt
     3781 gctgaaggcc atggcggagc cgacccggct gcgggtcttg gcgctgctcg cctacggcga
     3841 gctcaacgtc aaggatctga cgcgcgtcct gggccagagc caaccccgca tcagtcgcca
     3901 cctgaagctc ctgtgcgagg cgggtttggt cgagcgcgcg ccggaaggca gttgggtcta
     3961 cttccgcctg tccgagcaac ccatgggcag cgggctgccg catttggtct tgagcgaagt
     4021 cgatgccaac gaccccgtgt tgctgcgcga ccgtgcccgg gccgaggcgc tacgcgcgga
     4081 gcgcgaagcc atcgcgcagg cctatttcca agcccatgcc aaggagtggg acacgatacg
     4141 cgcgttgcac gttgccgagg ccagcgtcga ggcggccatc gcagccgccc tggagggcga
     4201 gccgctcggc ttcctgatcg atctggggac cggcaccggc cgcatgctgg aactgcttgc
     4261 ggcgcgctac cgccgcgggc tcggcatcga tcgcagtccg gcaatgctcg cctacgcccg
     4321 cgccaaactc gaacgcgcca agcttgccca cgcgcaggta cgccagggcg acatttacga
     4381 cctgccgctc ggcgacgggt tggccgatgt cgtcctcatg caccaagtca tgcatttttt
     4441 gggtgatccg ccgcaggcca tccgcgaggc tgcccgcgtc ttgcggcctg gcgggcgcct
     4501 gctgatcgtc gattttgcc
//
LOCUS       DASTMT010000033         2095 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_14538, whole genome shotgun sequence.
ACCESSION   DASTMT010000033 DASTMT010000000
VERSION     DASTMT010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2095
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14538"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..909
                     /locus_tag="VFR00_00725"
     CDS             <1..909
                     /locus_tag="VFR00_00725"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0157799.1"
                     /translation="RSPAEDPLSDDISIEFTRFSAFYSPLIATMAGGFLKDEGLKARY
                     AVSAPGKSAIAGLLDGSVHVAQSAPSQGFAPLEQGKQPPAVHFAQINERDGFFLAGRN
                     ADPSFSWKSLRGKKVIIDHGGQPMAMFKYACFKQGLDFGDLVPVDAGSTDKMIAAFRN
                     GEADYVHLQGPAPQQLVHDGVGHIVAALGDAIGPCAFSSLAAKREWLATDEAKAFMRA
                     YRKARAWLISTPAAEVAKVEASYFKDIDLSVLTATIASYQQLGNWTPHVEITRPAYAA
                     TLDIFQHAGLITRRHAYEDVVASPPA"
     gene            921..1784
                     /locus_tag="VFR00_00730"
     CDS             921..1784
                     /locus_tag="VFR00_00730"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU0157800.1"
                     /translation="MADTLGWIGVGSMGHRMSRHLVAAGHTLVVADAASTAQAPPQAR
                     IASSNAEVAGLADTIILSLPDGSVSEAVARELAVAAPRRVKTVIDTSTIGIKAAEAVN
                     SILAKVGVEFIDAPVSGGTAGADKATLAIMMACPSATFERCKPLLALMGKPFHVGPKP
                     GQGQAVKLLNNFLSATALAATCEAIAFGTQQGIEMKTILDIVNASSGRNSATDDKFPR
                     RIMHGKYDAGFAAKLQLKDIRLYLENARAAGIADEVASSVVGVWEKMNADLPAADITE
                     MYPYTRNGRRR"
     gene            complement(1833..>2095)
                     /locus_tag="VFR00_00735"
     CDS             complement(1833..>2095)
                     /locus_tag="VFR00_00735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0157801.1"
                     /translation="YTRRTGNAADGYFAIGDADIVLARLAEYIDAGICKFVLRPLGAD
                     GAEVMAQTRRLIAEVLPKVTARWPKPSKQASKQASKQAGKPA"
BASE COUNT          386 a          696 c          646 g          367 t
ORIGIN      
        1 cgctctcccg cggaggatcc cttgagtgac gacatcagca tcgaatttac ccgcttttcc
       61 gcgttctact cgccgctcat tgccaccatg gccggtggct tcctgaaaga cgagggacta
      121 aaggcgcgct atgcggtttc cgcgccgggc aaatccgcca tcgccgggtt gctcgatggc
      181 agcgtgcatg ttgcccagtc cgccccatcg cagggcttcg cacccttgga gcagggtaag
      241 cagccccccg ccgtgcactt cgctcaaatc aatgagcggg acggcttttt tctcgccggc
      301 cgcaacgccg atccgagctt tagttggaag agcctcaggg gcaaaaaggt catcatcgac
      361 cacggcggcc agccaatggc catgttcaag tacgcctgtt tcaaacaggg tctcgacttc
      421 ggcgacctgg taccggttga cgccggctcg accgacaaga tgattgccgc cttccgcaac
      481 ggcgaggccg attacgtgca cctgcagggt cccgcgcccc agcagctggt gcacgatggc
      541 gtcggccaca tcgtcgctgc cctcggcgat gccattggtc cgtgcgcctt ttccagcctg
      601 gcggccaaac gcgagtggct ggcaacggac gaggcgaagg ccttcatgcg cgcctaccgc
      661 aaggcccggg cctggctcat cagcacgccg gcggcggaag ttgccaaggt ggaggcctct
      721 tacttcaagg acatcgacct ttcggtgctg actgccacca tcgccagcta tcagcagctc
      781 ggcaactgga cgccgcacgt cgagattacg cggccggcgt acgctgccac tctcgacatc
      841 ttccagcatg ccggcctcat cacccggcgc cacgcttacg aagacgtcgt cgcttcgcca
      901 ccagcttgag gatctcgacc atggcagaca cactaggttg gatcggcgtc ggcagcatgg
      961 gccatcgcat gagccggcat ctggtggcag caggccacac cctcgtcgtg gccgacgccg
     1021 caagcacggc gcaagcgccg ccgcaggccc gcatcgccag cagcaacgcc gaggtggccg
     1081 gtctcgccga taccatcatc ctgtcgcttc ccgatggcag cgtcagtgag gcggtcgccc
     1141 gcgagctggc ggtggcagct ccgcgccggg tgaaaacggt aatcgacacc tccaccatcg
     1201 gcatcaaggc cgccgaggcg gtcaatagca ttctcgccaa ggtcggcgtc gaattcatcg
     1261 atgctccggt atcgggcggc accgccggcg ccgacaaagc gacgcttgcc attatgatgg
     1321 cctgtccgag cgccaccttc gagcgctgca aaccgctgct ggcactgatg ggtaagccct
     1381 tccatgtcgg tcccaagcca ggtcagggcc aggccgtgaa gctcttgaac aattttctgt
     1441 cggcgacggc gctggcggca acgtgcgagg cgatcgcctt cggcacgcag caaggcatcg
     1501 agatgaagac cattctcgat atcgtcaacg cttcgagcgg acgcaattcg gcgaccgacg
     1561 acaaatttcc gcgccgcatc atgcacggca agtacgacgc cggcttcgca gccaagcttc
     1621 aactcaaaga catacggctc tatctggaga acgcgcgcgc cgccggcatt gccgatgagg
     1681 tggcgagcag cgtggttggc gtgtgggaga agatgaacgc cgatctcccc gccgccgata
     1741 ttacggaaat gtatccctat acgcggaacg gacggcgccg ctagcgcgtc ggccgccgct
     1801 cccaccacac ctagagccga tcgtgcgggt agctaggccg gcttgcctgc ctgcttgctg
     1861 gcctgcttgc tggcctgctt ggatggcttg ggccagcggg ctgtgacctt gggtaaaacc
     1921 tcggcaatca ggcgacgcgt ctgagccatg acctcagcgc cgtccgcgcc cagcggacgc
     1981 aacacaaatt tgcatatgcc ggcgtcgatg tattcggcga ggcgggcgag aacaatgtcg
     2041 gcatcgccga tggcaaagta gccgtcggcg gcattgccgg tgcgccgcgt gtagg
//
LOCUS       DASTMT010000034         3323 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_4930259, whole genome shotgun sequence.
ACCESSION   DASTMT010000034 DASTMT010000000
VERSION     DASTMT010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3323
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4930259"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1896)
                     /locus_tag="VFR00_00740"
     CDS             complement(<1..1896)
                     /locus_tag="VFR00_00740"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEU0157802.1"
                     /translation="MQPEAKARLERCATAWQALVSAIALIFMGLVIGPGTSANAEEHL
                     KRLLIVYPDSSESHAAVIASEAVRSRFSQLASGLTVQTHTEFLDRTQFPGKEHKDRFV
                     QYLSDKFADSKPDAILAFGADSLEAAVSYRKLLATNTPIVFCVLPPAGAVVNFEPDMA
                     GIVSEHDVTKTVALARRLQPNARNLVVVAGTAAFDRKGALIAQQQLADTEKQLKTRYL
                     VGLPDETVERELASVPRNSIILLLSKLTEGPGRGAASGDAASIISAANAPIYAPYSTY
                     FGRGIVGGYMDTFDGMGTQTADLVLNILSGNESAASGPRKSTSQAFRVDARQLERWGL
                     SAQDLPKGTVVAHKPPTLWESHPRIVTATIAMFTAMASVLMALLVQIARRKLAEANLK
                     DSEKRMRFAAAAADIGLWQYDIQARRLWSSKHCRAMLGLPVREPLTTAALIGRVHPDD
                     RHVARASIRAATFGTLNDGESEFRVIWPNGETRWLQARGRTDLDENGEPLRASGIFRD
                     VTNYKIAQLEAKHLSQRVLSIQDDERQRIAQDLHDSTSQHLAAIGLNLIALKGSKGSC
                     SVLEDIENSLDEASKELRTFTYLLHPPALADDGLIGTLGRYVEGFKRRTNLCVSLRSA
                     GAIEELSR"
BASE COUNT          544 a         1009 c         1164 g          606 t
ORIGIN      
        1 ccgcgacagc tcttcgatgg cgcccgccga gcgcaagctg acgcacagat tggtgcggcg
       61 cttgaagccc tcgacgtagc gcccgagcgt gccgatcagg ccatcgtcgg ccagcgccgg
      121 cggatgcagc agataggtga aggtgcgcag ttccttggac gcctcgtcga gggagttttc
      181 gatatcctca aggacgctgc aggagccctt cgatcccttc agcgcgatca gattgaggcc
      241 gatcgccgcc aggtgctgcg aggtcgagtc gtgcaggtcc tgggcgatcc gctggcgctc
      301 gtcgtcctga atactcaaga cccgctggga caggtgtttg gcttcgagct gggcgatctt
      361 gtagttggtc acgtcgcgga aaatgccgct ggcccgcagc ggctcaccat tctcgtccaa
      421 atccgtgcgg ccccgggcct gcagccagcg cgtctcgcca ttgggccaaa tgacgcgaaa
      481 ttcgctctcg ccatcattca aggtgccgaa ggtcgcggcg cggatcgagg ctctggcaac
      541 gtggcggtcg tcgggatgca cccgcccgat cagggcggcg gtcgtcagcg gctcgcgcac
      601 gggcagcccc aacatggcgc ggcagtgctt ggaggaccac agccgccgcg cttggatatc
      661 gtactgccag aggccgatgt cggcggcggc ggcggcaaac cgcatgcgct tttcgctgtc
      721 cttgaggttg gcttcggcca gcttgcggcg ggcgatttgc accagcagcg ccatcagcac
      781 ggaggccatg gccgtaaaca tggcgatggt ggcggtgaca atcctcgggt ggctctccca
      841 cagggtcggc ggcttgtgcg ccacgacggt gcccttggga agatcttggg ccgacaaacc
      901 ccagcgctcg agttggcggg catcgacgcg aaaggcctgc gatgtgcttt tgcgcggccc
      961 gcttgccgcc gactcgttgc cgctcagaat attgagcacc aggtcggccg tttgcgtgcc
     1021 catgccgtcg aaggtatcca tgtagccgcc gacgatgccg cgcccgaagt acgtgctata
     1081 gggcgcgtag atcggcgcat tggcggcgct gatgatggag gctgcgtcgc ccgaggcggc
     1141 accgcggccg ggcccttccg tcagtttgga cagcagcaag atgatgctgt tgcgcggaac
     1201 gctggccagc tcccgttcaa cggtttcatc aggcaggccg accagatagc gggtcttgag
     1261 ctgtttttcg gtgtccgcca gctgctgctg ggcaatcagc gcgcccttgc ggtcgaaggc
     1321 ggcggtgccg gccaccacca cgaggttgcg ggcattgggc tgcaagcgcc gcgccagcgc
     1381 cacggttttg gtgacgtcgt gctcgctgac aatgccggcc atatccggtt cgaaattgac
     1441 gacggcacct gcgggaggaa gaacgcagaa aacaatcggg gtattcgttg ccagcagctt
     1501 gcggtagcta acggcggcct ccagcgagtc cgcaccaaag gccaaaatgg cgtccggttt
     1561 ggagtcggca aatttgtcgc tgagatattg aacgaagcga tccttgtgtt ccttgcctgg
     1621 aaattgcgtg cgatccagga actccgtatg ggtctggacc gtcaatccgg aagcgagttg
     1681 ggaaaatcgg ctgcgtaccg cctcgctggc gatgacggca gcatggcttt cgctgctatc
     1741 tgggtaaacg atcagcagcc ttttgaggtg ttcttcggcg ttggccgacg tgcccgggcc
     1801 gattacgagg cccataaaga tgagggcgat ggctgatacc agcgcctgcc acgcagtcgc
     1861 gcagcgctcc aaacgcgcct tcgcttcggg ctgcattggc ccgctccttt atccgtactg
     1921 cggttacgtg ggatccactg gtgaatagat ttccgactga aagcttagga catccgggat
     1981 tataccgagg tggcgtcgat aagctgtgta gtctctcaac ctcagttaac gcgggtcggc
     2041 ggcggcgctg acccagacat cccccggaac agtgggaaag gccccggaac agtgggaaag
     2101 gccgcctaaa gagcggcggc ggcgatgcgg gatggcattt tagggcaatc ggaggggccg
     2161 tggggcaagg agcgcggacc taggacaaca tgaggggagc ctaaggcaaa aagaggggtt
     2221 gcacgcggtc tcggtggcgc cgcggcgctg cccgggcacc gcgacgcggc gacccgcagg
     2281 cgaccacacg cctcgcgctc tcggccttgt gccaacgtcg ggtactgagg cgtgtgcgca
     2341 agggcgctat gcggtcggcg agggctcgcc cgcgcacgaa gcctgctgcg agtgctcgag
     2401 gctcgaggca ccttgccgtc gttttcggcg agcttgatca tgcgccgcca aacctcatcg
     2461 ccgccatctc attgcggctg ccggtgtcgt ggagctcggt tgccaggcgc tagcgcgcgg
     2521 cgaggactgc gtcgcgtttg agaggattct gctggcgatg ttgccgcgag cccgcatgac
     2581 cacggacgag cccgggggag gacacgcgcg gggccggctt gggggaagcg cgccgcgcag
     2641 tggcggcggc tgccctcctg cgggcggcgg atgggcggcg agaagctcgc ccgcggcgcc
     2701 tgtgaaactc gacgacatga cgcgtgtgaa cacccgacat attttctcct cccatgcgtc
     2761 agtgccttct cacgggcgcg tcattccggc gcacgaggtc agacgcggca agcggcgatg
     2821 cgtgcgaaag aaggcgtcat tcggggcggt ccccgagctc ggcgcttggg cctcaggcgc
     2881 ggcgcgcgct ggcagaaatg cgccgcggcc cgaggcgcgg ccgcgcactg caccgatgcc
     2941 tcggaacctt gtccttccat tggccgttcc cgctgcgagc gcccgcgcgt gtcaatgacg
     3001 gaagcgtggg ggttggcgcg tgcccgagaa atgtcccaac gtgtggccgg cgtgcggcca
     3061 gcctgcagcc agcctgcagt cagtgtgcgg gggcgcggtt caacgaggat ggtcgcgcgg
     3121 ggcttttgag gtttgcggcc cgtgcgatgg tggcctgagg aggtggcgga catgtctcgc
     3181 gcagatcgcg gttttgccct gttcttgatg gtggtgctgg tcgctctatc cgctttgttt
     3241 tttgaactgt tcccgcggca cggggagcaa aatcttgccg gtgagggcgc cacgacatcg
     3301 gccggcacgc catcgccacc tgc
//
LOCUS       DASTMT010000035         1874 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_999630, whole genome shotgun sequence.
ACCESSION   DASTMT010000035 DASTMT010000000
VERSION     DASTMT010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 1874)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1874)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1874
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_999630"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..445
                     /locus_tag="VFR00_00745"
     CDS             <1..445
                     /locus_tag="VFR00_00745"
                     /inference="COORDINATES: protein motif:HMM:NF024241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0157803.1"
                     /translation="FAYSAALIGRVGPGALLAIAAGSAVIRWTAMAFDPPVWLLYGLQ
                     TLHGLTFGATHVGAIHVLARLAPPTQGGTAQALYAAVTSGILLGGATLLVGPLYAAYA
                     AGAYFAMATLAAIGFFAALGLARRPEPAAGPGRLAASRPAEDLDA"
     gene            613..1863
                     /locus_tag="VFR00_00750"
     CDS             613..1863
                     /locus_tag="VFR00_00750"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009800050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEU0157804.1"
                     /translation="MAGTDVIVLGAGMVGVSTALHLQKRGQAVVLVDRRGAGEETSYG
                     NTGIIQREGVVPYPFPRDVRLLLRYAFNQLPESHLDWRALPVIAPWLFRYWRASTAEG
                     VAATSRRARPLIERCVLEHETLMGEAGVLGLMRRTGYLRLYHSAPALDSAMLKDKAAS
                     ETYGVKFEMLSASALGAMEPHLKAPLAGAMFMPDPVSVSDPEAVTKAYARLFQERGGR
                     FQRGEAGTLQATPVGWEVQTATGSVTAAAVVIALGPWSNTLFRRLGYRIPFGVKRGYH
                     MHFRAEGNATLNRPVIDAEHGYALTPMTRGIRLTTGAEFALRDAPPTPVQLARVEPLA
                     RRIFPLGERLDAEPWLGRRPCLPDMLPAIGRGGRHSGLWFNFGHHHLGFTLGPVTGRL
                     LAEMMTGETPFTDTAPYGPERFGS"
BASE COUNT          262 a          652 c          632 g          328 t
ORIGIN      
        1 gtttgcctat tcggcggctc tcatcggccg tgtcgggccc ggcgccctat tggcgatcgc
       61 cgcgggcagc gccgtgatcc gctggacggc gatggctttc gaccccccgg tctggctgct
      121 ctatgggctg cagaccctgc acggcctgac ctttggcgcc acccacgtcg gcgccattca
      181 cgtcctggcg cggctagcac cgccaacgca gggtggcacg gcgcaggcgc tttatgccgc
      241 ggtcacctcg ggcatcctgc tcggcggcgc caccctcctg gtcgggccgc tctatgccgc
      301 ctacgctgcg ggcgcctatt tcgccatggc gacgctggcg gcaattggtt ttttcgccgc
      361 cctcggcctc gcccgcaggc ctgagccggc ggcgggcccg ggccggctgg cggcctcgag
      421 gccggccgag gacctcgatg cctgagccga cgctgcgcct ttgcgccggc acgaaaaggc
      481 ttcgctgccc actcggctcg gttgccgagg ggcgatttgc cgtataggcg aaatggccct
      541 gtccagccca tgggcaggca cctgcgaaca gggctgcggc gccaggcgag gacggcaggg
      601 gagggagctt ttatggctgg aaccgatgtc atcgtgctgg gcgccggcat ggtcggcgtg
      661 tcgacggcgc tgcacctgca aaaacgcggc caggccgtgg tgctggtcga ccgccgcggc
      721 gcgggcgagg aaacgagcta cggcaacacc ggcattatcc agcgtgaagg cgtggtgccc
      781 tacccgtttc cgcgcgatgt tcgcctgctc ctgcgctacg ccttcaacca gctgcccgaa
      841 tcgcacctcg attggcgcgc gctgcccgtc attgccccct ggctctttcg ctattggcgg
      901 gcctcgacgg cggaaggggt cgccgccacc tcgcggcggg cacggccact gatcgagcgc
      961 tgcgtgctcg agcacgagac cttgatgggc gaagccggtg tgctcggctt gatgcggcgc
     1021 accggctatc tgaggctcta tcattccgcg ccggcgctcg acagcgccat gctcaaggac
     1081 aaggcggcga gcgagaccta tggcgtcaag ttcgaaatgc tgagcgcaag cgcgctggga
     1141 gccatggagc cacatctcaa ggcccccctg gcaggcgcca tgttcatgcc cgatcccgtc
     1201 agtgtctcgg atcccgaggc ggtcaccaag gcctatgccc gcctgttcca ggagcgcggt
     1261 gggcgctttc aacgcggcga ggccggtacg ctgcaagcaa cgccggtggg atgggaagtg
     1321 caaacagcga ccggcagcgt cacggctgct gccgtggtga ttgcgctcgg cccctggtcc
     1381 aacaccctct ttcgccggct aggctaccgc attcccttcg gcgtcaagcg tggctatcac
     1441 atgcactttc gggccgaggg caacgcgacc ctcaatcgac cggtgatcga tgccgagcac
     1501 ggctacgcct tgacgccgat gacgcgcggc atccgcttga ccaccggtgc cgaattcgcc
     1561 ctgcgcgatg ccccccccac gccggtgcaa cttgctcgcg tcgagccctt ggcgcgccgc
     1621 attttcccgc tcggggagcg gctcgatgcc gaaccctggc ttggccggcg tccctgcctg
     1681 cccgatatgc tgcccgccat cggtcgcggc gggcgccaca gcggattgtg gttcaacttc
     1741 ggccatcatc atttgggctt cacgctgggg ccggtgacgg ggcgcctgtt ggcggaaatg
     1801 atgaccgggg agacgccctt cacggacacc gcgccttacg gtcccgagcg cttcggctca
     1861 tgagcggttg agat
//
LOCUS       DASTMT010000036         4464 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_345696, whole genome shotgun sequence.
ACCESSION   DASTMT010000036 DASTMT010000000
VERSION     DASTMT010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4464)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4464)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4464
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_345696"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            329..691
                     /locus_tag="VFR00_00755"
     CDS             329..691
                     /locus_tag="VFR00_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0157805.1"
                     /translation="MRDMYGHVNRATLHRAGIDFMLALGLFWLLTFALSSHDSRAHAV
                     PLYAQPQAHEIAAERVNRLTLLSFEARSNRLTPPDSRQSLGLLSLAFALLAALNLAIW
                     RHLRRAYASPRRGVWRRG"
     gene            798..1295
                     /locus_tag="VFR00_00760"
     CDS             798..1295
                     /locus_tag="VFR00_00760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0157806.1"
                     /translation="MQRTHWKTRVTMLTACLIAGAAGAAVLRTDVLLGRSLGTALQQS
                     SSELSFDGVQSPARAGIVGDEGFWLTRASSDASFPLAKPLLVGDHISISSAGGDNRQF
                     EVTAMKALGPDGAPSAEGNLAPSRLMLVVCRLIDATDRTPRFVRFIVEMPAPEATRPV
                     QSKAL"
     gene            1506..1691
                     /locus_tag="VFR00_00765"
     CDS             1506..1691
                     /locus_tag="VFR00_00765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0157807.1"
                     /translation="MRLNPPSLFIFSISLLLAGLALISKFGAIGINVPRYLPHQEYWL
                     AMMAYLVLMVGNLVRGL"
     gene            2157..3299
                     /locus_tag="VFR00_00770"
     CDS             2157..3299
                     /locus_tag="VFR00_00770"
                     /inference="COORDINATES: protein motif:HMM:NF015056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HEU0157808.1"
                     /translation="MKRSLPVSASIGALFAIVAASIVAAPSAAQQKSPARGNHDWAPK
                     VAQGKHHTLPASLDTVQWGWLDPREKPKLTINSGDTVSIETMMHSHDKIQPGTTMEQV
                     VELRKANPGGGPHSMTGPIYVNGAEPGDVLEIRILRIEPKGWATNFNLPGRDFPTIGT
                     LAAEMPEGHIKFFPIDTQKRAVEFKPGIRIDLQPFPGTLAVGIDPNDPSPRKGGVKDD
                     PMAPVSTLRPWKNGSNMDINELQEGSTLFIPVFLQGGLLWTGDSHCRQGNGEVNLTAL
                     ECSYKEIRLQPIVRKDMKLNWPFVETRTHWITTGFNEDLNVAMTNAVREAVDWLANQK
                     MVPMSRYEAYGLVSMVGDCRVSQVVDVRKGVHCMIPKSIFTDQKKS"
     gene            3296..4180
                     /locus_tag="VFR00_00775"
     CDS             3296..4180
                     /locus_tag="VFR00_00775"
                     /inference="COORDINATES: protein motif:HMM:NF013465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HEU0157809.1"
                     /translation="MSRVKRRLALALASILLATPQGVAAQEKIRVVTTSTDLKSLVEA
                     VGGERTEVESLTLPEQDPHTVEIKPTQLARLGKARLIVRIGLDHEPWFGRLPVHDVPV
                     IDASRSVKLLQTQTPRLRTERQAHVHAFGNTHYWLDPDNAAPITAAVLAALAAQDPAA
                     EGTFAKNRQAFLARLNDRILAWDAALKGLRGTKVVVVHDSWAYFAERFGLNIVAAAEP
                     SPGVTPSPGELAALFKRMREANVRLLIADPHTNPALVQQIIDRGGARAVTLLPSSADY
                     IGLFEENVRRLSLLLNPG"
     gene            4186..>4464
                     /locus_tag="VFR00_00780"
     CDS             4186..>4464
                     /locus_tag="VFR00_00780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEU0157810.1"
                     /translation="MLELLLWPFIAGLVLTVIHAYFGLHVLARGVIFVDLSLAQVAAL
                     GLTIAILVGHPVNSAGSYWYALLFAVGGAVVFAVARPYEQALPQEALIG"
BASE COUNT          774 a         1497 c         1408 g          785 t
ORIGIN      
        1 acccgctcct ttgctgggac tgaccctcgg aaaagttgta ggccaatcca atcgcgctca
       61 caacggacag accgcgtctt tgtgcaccgg aaatcagctt cacgggcgcg tgagacggca
      121 cgaggcgaag aagcgacgga ttttttgcgg ccccggcgtt cgtttgccgt tgggaacacg
      181 cgacccgagg gcacttaatt ccgacacagg atcaaacggc tcggccatat tgtcggcgca
      241 tccacgccct actgacctgc ttggatgcct ttgcgccaag gggccgggtc ggatcgggga
      301 gccggcacca agcgcgaggg agtgcgggat gagagacatg tacgggcatg tcaacagggc
      361 tacgctccac cgagcgggca tcgacttcat gctggcgctg gggttgtttt ggctgctgac
      421 cttcgcgctc agctcccatg acagtagagc tcacgccgtg ccgctctacg cccagccgca
      481 agcccacgag atcgccgcag aacgggtgaa ccgtttgacc ctgctcagct tcgaggcgcg
      541 cagcaaccgg ctcaccccgc cggatagccg ccagtcccta gggcttctca gcctggcctt
      601 tgcgcttctg gcagcgctca atctcgccat ttggcgtcat ctgcgccgcg catatgcctc
      661 cccgcggcgt ggtgtgtgga ggaggggatg acaggtcagg gtggtcttct cccccaccct
      721 cctccacact tttgcaatgt tgctgtagtg ttgtggcaag accgaacgga cttcaggcaa
      781 tgaaacaaag ggcaacgatg cagcggaccc actggaagac ccgcgtgacc atgctgacgg
      841 cgtgcctgat cgctggtgcc gccggcgcgg ccgtgctgcg caccgacgtg ctgctcggcc
      901 gcagcctcgg cacggcgctg cagcaatcga gctccgagct ttccttcgac ggcgttcagt
      961 cgccggcaag agccgggatc gtgggcgatg aagggttctg gctgacgcgg gcgagcagcg
     1021 acgcctcgtt cccgcttgcc aagccgctct tggtcgggga tcacatctcc atctccagcg
     1081 ccggcggcga caatcggcaa ttcgaagtga cggcgatgaa ggcgcttggc cccgacggcg
     1141 cgcctagcgc ggagggcaat ctggcgccga gccgcttgat gctcgtcgtc tgccggctga
     1201 tcgatgccac cgatcgcacg ccccgctttg ttcgcttcat tgtcgagatg ccggcccccg
     1261 aagcgacaag gcccgtgcaa tcgaaggccc tctagcgggc tcccggcgcc agcgtgcaac
     1321 ggctggcgtg gtgaggagaa gcgcatcgcc ttgccaggct cgtagggcgc acgcgcgcag
     1381 tgcctcccgg gggccacggc ccgcgcctcg caacttccgg ccatatcgct atttccgcgc
     1441 ggcacgggag cccctacact tgcggccagc catgctggcc ttgccgctct ggagggcgcg
     1501 tccttatgcg tctcaacccg ccgtcgctct ttatcttttc gatttccctg ctgttggcgg
     1561 gcctggcgct gatttccaaa ttcggcgcga tcggtatcaa cgtcccgcgc tacctgccgc
     1621 accaggaata ttggctggcc atgatggcct acctcgtgtt gatggtcggc aacttggtac
     1681 gcgggctcta gccgagctcg gctccgtgtc atcttcaagt tggcatcttc aagccgttgc
     1741 cggggcaaga cgtggcaccg cccggcggcc gccccgatcc agggcgcacc agagtgtcct
     1801 cgaccggccg cgccacagcc gtccgcttgc cggcaagccc cggcaattga gccgccaagg
     1861 ccgcccatcc ccccacgcgt ttcaaggcca aaagaccgct tccggtcagc tcggtcccgc
     1921 cggcgccgac ggcaaagccg gtgccgagct ccgggagtgc cgcggctcgg cggcgcccgc
     1981 tggcgcgcca atacccgcca aaccgcgcga cttttgcgca ttttcgcggg ccgtgtgacc
     2041 gcatcgggcc gccgccggtg ccggcgacag gcgaccccag ccccgttggg agtgacaggc
     2101 ttgacgagcg gattgccggc aattcaccat gcgccgagcg ggaggaggtc tccacgatga
     2161 aacgctcact accggtatcc gccagcatcg gcgccctgtt cgccatcgtc gctgccagca
     2221 tcgtcgccgc gcccagcgcg gcgcagcaaa aatcgcccgc gcggggcaat catgactggg
     2281 cccccaaggt ggcgcagggc aagcatcaca cgctccccgc cagcctcgat acggtgcagt
     2341 gggggtggct cgatccgcgc gagaagccca agctcaccat caactcgggc gacacggtct
     2401 cgatcgagac catgatgcat tcgcacgaca agatccagcc gggaacgacg atggagcagg
     2461 tcgtcgagtt gcgcaaagcc aatcccggcg gcgggccgca ctcgatgacg ggtccgatct
     2521 atgtcaacgg tgccgagccg ggcgatgtcc tggagatccg cattcttcgc atcgagccca
     2581 aaggttgggc gaccaacttc aatctgccgg gcagggattt cccgaccatt ggcacgctcg
     2641 ctgccgagat gccggaaggt cacatcaaat tcttcccgat cgacacgcaa aagcgcgccg
     2701 tcgagttcaa acctggcatc cgcatcgact tgcaaccgtt cccagggacg ctcgcggtcg
     2761 gtatcgaccc caacgatccc tcaccccgca agggcggcgt gaaggacgat ccgatggcac
     2821 cggtgtcgac gctgcggccc tggaaaaacg gctccaacat ggacatcaac gagctgcagg
     2881 aaggctcgac gctgttcatc cccgtgttct tgcaaggcgg cctgctgtgg acgggggact
     2941 cccattgtcg gcaaggcaat ggcgaggtga acctcacggc gctcgagtgc tcctacaaag
     3001 agatccggct gcagccgatc gtacgcaagg acatgaagct caactggccc ttcgtcgaga
     3061 cccgaacgca ctggatcacc acgggcttca acgaagacct caacgtcgcc atgaccaacg
     3121 ccgtgcgcga ggccgtcgac tggctggcaa accagaagat ggtgccgatg agccgctacg
     3181 aagcctacgg cctcgtgtcg atggtgggtg actgccgggt gtcacaggtg gtcgacgtgc
     3241 gcaagggcgt gcactgcatg attccgaagt cgatcttcac cgaccaaaag aagtcttgag
     3301 ccgggtcaag cgaaggttgg cgctggcgct ggccagcatc ctgttggcga cgccgcaagg
     3361 cgtcgccgcg caggaaaaga tccgggtcgt caccacgtcc accgatctga agtcgctcgt
     3421 cgaggcggtc ggcggcgagc ggaccgaggt ggaaagcctg accctgcccg agcaagaccc
     3481 gcacacggtc gagatcaaac cgacacagct ggcccgcctg ggcaaggccc gactgattgt
     3541 caggatcggc ctcgatcacg agccttggtt cggccgcctg ccggtgcacg acgtccccgt
     3601 gatcgatgcc tcgcgctcgg tcaaactgtt gcagacacaa acgccgcgct tgcgcaccga
     3661 gcgacaggcg cacgtgcatg ctttcggcaa tacgcactat tggctcgatc ccgacaatgc
     3721 cgcgccgatc accgctgcgg tgctggcggc gcttgccgcc caggatccgg cggcggaggg
     3781 cacatttgcg aaaaatcgcc aggcgttcct ggcccgcctc aacgatcgga tcctcgcctg
     3841 ggacgccgcc ctcaagggct tgcggggcac caaggtcgtg gtcgtgcacg acagctgggc
     3901 ctatttcgcc gagcgtttcg gcttgaacat cgtggcggcg gccgagccct cgcccggggt
     3961 gacgccgtcg ccgggggagc ttgctgccct gtttaagcgc atgcgcgagg caaacgtgcg
     4021 cctcctgatc gccgaccccc ataccaatcc cgccctggtg cagcagatca tcgaccgcgg
     4081 cggggcaagg gcggtgacct tgttgccatc ctcggcggat tatatcggcc tcttcgagga
     4141 gaacgtgagg cgtttgtccc tgttgctcaa tccgggctga aggcgatgct cgagctgctg
     4201 ctgtggcctt ttatcgccgg cttggtgctg acggtcattc acgcctattt cggccttcac
     4261 gtgctggcac gcggcgtcat tttcgtcgat ctttcgctgg cgcaagtggc tgcccttggt
     4321 ctcaccatcg ccatcctcgt cggccatccc gtcaacagcg cgggaagcta ctggtatgcg
     4381 ctcctgttcg ccgtcggcgg cgccgtcgtg ttcgccgtag cgcgccccta cgagcaggct
     4441 ttgccacaag aagcgctgat cggc
//
LOCUS       DASTMT010000037         1613 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_1330497, whole genome shotgun sequence.
ACCESSION   DASTMT010000037 DASTMT010000000
VERSION     DASTMT010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 1613)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1613)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1613
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1330497"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1290)
                     /gene="apbC"
                     /locus_tag="VFR00_00785"
     CDS             complement(<1..1290)
                     /gene="apbC"
                     /locus_tag="VFR00_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009210427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HEU0157811.1"
                     /translation="MSVSREHILERLRRVKGPDLESNIVDLGLVSEVLVRDGRVSFSI
                     TVPAKRGAELEPLRQAAETIVREIDGVSHVTAVLTAERARGSAPDRGPTHTGHAPESQ
                     RVREARAMAAGHRPPPSPPPAAAAPPARAIGKVAGVRHLIAVASGKGGVGKSTTAVNL
                     ALGLQSLGLKAGILDADIYGPSQPRLLGLTGRPQVTGGKTLRPMEGFGLQAMSMGFLV
                     DEATPVIWRGPMVVSALTQMLRDVAWGDLDVLVIDMPPGTGDVQLTMAQQVPLSGAII
                     VSTPQDLALIDARKGLNMFRKVDVPVLGIVENMSTFICPRCGERSDIFGHGGAEREAD
                     KLGIPFLGGVPLHMEIREKSDAGQPIVASNPDSPHAQVYRDIAARAWQQLEESVEQRL
                     EPPKLAVSADHSALEVVFNDAAPVLLTAEMLRVMSPSA"
BASE COUNT          252 a          563 c          535 g          263 t
ORIGIN      
        1 ggcggacggg ctcatgaccc gcagcatctc ggccgtcaac agcaccggcg cggcatcgtt
       61 aaagaccact tccagcgcgc tgtgatcggc gctgacggcg agcttgggcg gctcgagccg
      121 ttgctcgacg ctctcctcca actgctgcca ggcccgggcg gcaatgtcgc gatagacctg
      181 ggcgtgggga ctgtcggggt tgctggcgac aatcggctga ccggcatcgg atttttcccg
      241 gatctccatg tgcaacggca caccgccgag gaagggtatc cccagtttgt ctgcttcacg
      301 ctcggcgccg ccatgaccaa aaatatcgct gcgttctccg cagcgcgggc aaatgaaggt
      361 gctcatgttc tcgacgatgc cgagcacggg aacgtcgacc ttgcggaaca tgttgaggcc
      421 cttgcgggcg tcgatcaatg ccaggtcctg cggggtggag acgatgattg cgcccgacag
      481 cggtacctgc tgggccatgg tgagctgcac atcacccgtg cccgggggca tgtcgatcac
      541 cagcacgtcg agatcccccc aggccacgtc ccggagcatc tgcgtcaagg ccgacaccac
      601 catgggaccg cgccaaataa cgggagtggc ttcgtccacc aaaaagccca tcgacatggc
      661 ctgcagtccg aagccttcca tcggccgcaa ggtcttgccg cccgtcacct gcggccggcc
      721 ggttaggccg agcaggcgcg gttgcgacgg gccatagata tcggcgtcga gaatgccggc
      781 cttgagaccg agcgactgca agccgagcgc caagttgacc gccgtcgtcg acttgccgac
      841 gccgcccttg cccgaggcga ccgcgatcaa gtgccgaacg ccggcgacct taccgatggc
      901 acgcgccggc ggcgccgcag ccgctggcgg cggcgacggc ggcggccgat gaccggcggc
      961 catggcgcgg gcctcgcgca cgcgctggct ctcgggggcg tggccggtgt gcgtcgggcc
     1021 cctatcgggc gcgctgccgc gggcgcgttc ggcggtcagc acggccgtca cgtggctgac
     1081 gccatcgatc tcgcgcacga tcgtttcggc ggcctggcgc aagggctcaa gttccgcgcc
     1141 gcgcttggcc ggcacggtga tggagaaaga cacccgccca tccctgacga gaacttccga
     1201 cacaaggccg aggtcgacga tgttgctctc aaggtcgggg ccctttaccc ggcgcaggcg
     1261 ctcgagaatg tgctcccggc tgacgctcat cgatgtctcc ttttactccg cgcgcacacc
     1321 tgccagcgcc gcactcgacg ctcaagcttg ccccatcgga ttgcgctagg cacctgggcc
     1381 gctgcaccag ggccgctgca cctgggcccc tgcacctggg cccctgcacc ctgggccccc
     1441 tgcgcccgga gggcggcgcc tcgaaccgac cttgagcctg gcaaacacgc cccgcccgtt
     1501 gagctggccg ggttgccgcg cccgcgccag cgtgacggcc cgcgctcccg cgtgaggcta
     1561 aagggtcttc ttgcgttgct ggatcatcat gtaggtgtcg taagtgttgt ccg
//
LOCUS       DASTMT010000038        11278 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_673968, whole genome shotgun sequence.
ACCESSION   DASTMT010000038 DASTMT010000000
VERSION     DASTMT010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 11278)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11278)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11278
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_673968"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1540
                     /locus_tag="VFR00_00790"
     CDS             <1..1540
                     /locus_tag="VFR00_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013420885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HEU0157812.1"
                     /translation="PAEAREQMMARDWPARDIGPLVDLIADPRHRLADTGTIRLSDEQ
                     ARAILDLRLQRLTALGRDEIGDELKKLGEEIKNYLAILASRARIVGLIKDELTAIKTE
                     FATPRLTEIVEMEGELEDEDLIQREDVVVTVSHRGYIKRVPLSTYRAQRRGGKGRAGM
                     STREEDVVTQIFIASTHTPVLFFSSRGMVYRMKVWRLPAATPQSVGKALVNLLPLSEG
                     ESITSILPLPEEEKTWGSLELMFATRSGSVRRNSLADFENINRNGKIAMKLEEGDRIV
                     RVAICTPSDNVLLTTAGGRCIRFPVEDVRLFKGRDSSGVRGIRLEDDDEVISMAILRH
                     VEASPGERAAYIKQALAVRRAQGAEIADATVEPAAEVEAEEGEEAVDLSPDRYAELGA
                     REQFVLTVSERGFGKRSSSYEYRISGRGGKGITAMIVNERNGRLVASFPVEESDQIML
                     VTDGGKLIRCPVDDVRIAGRNVQGVRIFRTDDDEKVVSVERISEEGNGDGAANGGEEA
                     EPTA"
     gene            complement(1660..2178)
                     /locus_tag="VFR00_00795"
     CDS             complement(1660..2178)
                     /locus_tag="VFR00_00795"
                     /inference="COORDINATES: protein motif:HMM:NF028004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P460 family protein"
                     /protein_id="HEU0157813.1"
                     /translation="MKALPIAATLALAAVTLTVGVRAGGEKVAFPENYAKGVLYTTVD
                     RADNKQYRELYATPEAIAAVKAGQPIPSGSVLTLVQHKAKLDADGNPEKDANGRFIKG
                     ELIGYTVMEKRTGWGSEYPDEQRNGEWEYQAFKADKTVNDKANLKNCFTCHKPLDKQD
                     FVFSFGAMKTAK"
     gene            2369..2986
                     /locus_tag="VFR00_00800"
     CDS             2369..2986
                     /locus_tag="VFR00_00800"
                     /inference="COORDINATES: protein motif:HMM:NF013933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HEU0157814.1"
                     /translation="MTVEVLFAFIAACLLLGLTPGPNMSLIIANTLSTGLTAGLFTLA
                     GTTTGLALLVGAAVMGMSSVMVFMSEWFDVLRFLGALYLVWLGARQLWQFWFAAPGAA
                     APPASRGNCYAQGLLVSLSNPKVMLFLGAFLPQFIDPGADAVGQLTLLAVLFVVVLAA
                     VDVGYTLLLARARTRFSMARLRWLDGAAGVLLLLGGAVLATMRRP"
     gene            3120..3638
                     /gene="coaD"
                     /locus_tag="VFR00_00805"
     CDS             3120..3638
                     /gene="coaD"
                     /locus_tag="VFR00_00805"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEU0157815.1"
                     /translation="MKRIGFTSGSFDPVTNGHRDVIERAARLVDTLVVGIGVHPGKTP
                     LFSVAEKIELLKAETAPIAKATGSRIEIVTFDNLTVEAAAAAGAGVIFRGLRDASDFD
                     YEMQMAGMNGSMAPAIQTVFLPASPQVRHITGTLVRQIALMGGDVSKFVSAGVARKLK
                     AKARAKQEIVKG"
     gene            3830..4333
                     /locus_tag="VFR00_00810"
     CDS             3830..4333
                     /locus_tag="VFR00_00810"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006933367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEU0157816.1"
                     /translation="MFASVSLLQAQATDPQNTLVIETTKGPIRIKLRPDLAPKHVAQI
                     KQLAKQGFYDGVIFHRVIAGFMAQTGDPTGTGRGGSKLPDVPAEFGSEPFKRGSVGAA
                     RSSNPNSANSQFFICFGDGCRQLTGQYTLWGEVIDGMDNVDKIARGEPPPQPDKMQKV
                     YLQADKK"
     gene            4387..4860
                     /locus_tag="VFR00_00815"
     CDS             4387..4860
                     /locus_tag="VFR00_00815"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019402697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEU0157817.1"
                     /translation="MTLEHDPENTLIMETTKGKVVIGLRPDLAPKHVERIKKLVREGF
                     YDGVAFHRVIDGFMAQTGCPEGTGMGGSNYPDLKAEFNAEKHVRGVCSMARSTRPDSA
                     NSQFFICFDDATFLDKKYTVWGKVLEGMENVDKLERGEPVAKPDKIRSLKVAADA"
     gene            5016..5783
                     /locus_tag="VFR00_00820"
     CDS             5016..5783
                     /locus_tag="VFR00_00820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0157818.1"
                     /translation="MRYFMSFWLGFALAAGLWLASGWRGDGAHADFTDLAGVEDWMAH
                     YHLKPEPRRLPQAIETLARAGALEGEARLKPVAAFVAALIGEDDATLARLSEAIAEAR
                     PAKQRLLAEAIALSGRPQWRRLLTELKRAVPARALEIETLLAAPEAPVTLAAAFDDAG
                     VVVDMVFAHFMATGSEAAFQRILAALAGHFAADDPAAASSAEKAKVTLSSRVGDDPRL
                     LELVRGEAEAEDEPLARLLRGVLVAAAQQARGRPSGN"
     gene            5834..6976
                     /gene="queA"
                     /locus_tag="VFR00_00825"
     CDS             5834..6976
                     /gene="queA"
                     /locus_tag="VFR00_00825"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010909613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEU0157819.1"
                     /translation="MRAELFDFELPEDLIALEPAEPRDAARLLVVCPDAGVAAAPTPA
                     PASPPEQRGALRLTDTRLERLADLLRPGDALVLNDTRVIRAALRGERLRGEQHAVVSF
                     NLHKRLDESRWLAFARPAKRLRRGDRIRFGHDGRVCLLGQLDATVTALGDAGELELSF
                     SLHGPYLDEAIEALGDPPLPPYIASRRGYVPEDAERYQTVYARHAGAVAAPTAGLHFT
                     PAVFAALEARGISRHFLTLHVGAGTFLPVKADDTTDHRIEAEWGQISADSARALNAVR
                     AAGGRIVAVGSTALRLLETAADRDGRLGAFSGETQLFITPGYRFKAVDLLLTNFHLPR
                     STLFMLVAAFSGLENMHAAYRHAIQARYRFYSYGDACLLYPADASR"
     gene            6973..8100
                     /gene="tgt"
                     /locus_tag="VFR00_00830"
     CDS             6973..8100
                     /gene="tgt"
                     /locus_tag="VFR00_00830"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012111982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEU0157820.1"
                     /translation="MTPFSFQLRHTDAGARLGEITTARGVIATPAFMPVGTAGTVKAI
                     YTDQLKAAGVDVVLANTYHLMLRPGAERIARLGGLHTFMRWERPILTDSGGFQVMSLA
                     KLRKITEEGVAFQSHIDGARHLLTPERAVEIQCLLGSDIQMQLDECVGLPASHDALAV
                     AVERSLAWARRAQRAFLAKRADGRAHLGQELFGIVQGGTHRDLRARSVDGLIDLGFAG
                     YAVGGLAVGEPQQAMLETLEGTLPRLPAEKPRYLMGVGTPLDLIEAVARGVDMFDCVL
                     PTRSGRHGQAFTWGGKLNLRNAVNSEDRAPLDASSQCPAARDYSRAYLHHLVKAEEYL
                     AAMLLSWANVAFYQELMAAMRQAIAEGRFAAWAAATKAALA"
     gene            8214..8384
                     /locus_tag="VFR00_00835"
     CDS             8214..8384
                     /locus_tag="VFR00_00835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0157821.1"
                     /translation="MSAEQQGRKAKWRALAAEARACAAETSEPRARRILQEIAAAYER
                     LAIGEPPQKRPS"
     gene            complement(8477..9607)
                     /locus_tag="VFR00_00840"
     CDS             complement(8477..9607)
                     /locus_tag="VFR00_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008190238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate-amine ligase"
                     /protein_id="HEU0157822.1"
                     /translation="MALKDPSFSIGIEEEYLLVERTTRDLVREMPQALFEAAQEAMQG
                     QVAREFLKSQIEVGTSVHHTPRQAGAELAHLRRTVAGLAAEHGLAPIAASTHPFARWS
                     TQEPTERARYQAIAKDLAGVGRRLVICGMHVHVGIEDDELRIDLFNQARYFLPHLLML
                     STSSPFAEGEDTGLKSYRLAAYQELPRTGMPGRFEGWYEYLQTVDLLVRNGIIEDASK
                     IWWDIRPSSRFPTLEMRVTDVCTLIDDAVCVAALYLCILRMLYRLRRANQTWRTYPLF
                     LLAENRWRAQRYGVEGTLFDLGKGELVAFRDLVDELLVMLAEDAAALGCSADLEHART
                     ILARGTSADRQVKHFLARVAAGATREEALRSVVDHLIEETVA"
     gene            complement(9712..10608)
                     /locus_tag="VFR00_00845"
     CDS             complement(9712..10608)
                     /locus_tag="VFR00_00845"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEU0157823.1"
                     /translation="MSAPDDAAAVTVSPFSITAADGYSLAATLFQSGAAAERAPITVI
                     SPAAAVPARYYARFASYLAERGGLVVSFDYRGIGASSPPSLRGFAGRMRDWCILDVPG
                     VIAWAGRAHPERPLHWLGHSMGGFAPGLAHNNAAIARQLNIATLSGYWRRMRSPERYR
                     VRVLMGIVAPPLVRALGYFPGVFMGGENMPGPAFLEWAGWCMTPDFIFGDPTLPEVRH
                     FATLRAPVRFAQIEDDVWGTPAAVGHMANHFKANSERSIWSVRLADAQATKIGHLGFF
                     RPTFRTTLWPAAAAWLEAGAPA"
     gene            10795..>11278
                     /locus_tag="VFR00_00850"
     CDS             10795..>11278
                     /locus_tag="VFR00_00850"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013503206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEU0157824.1"
                     /translation="MLAAWKPDSWRSKPIVQVPEYPDPLALADVEHNLRNFPPLVFVK
                     EVRDLKEKLAGVAEGKAFLLQGGDCAESFKEHKTDYIRDYFQLFLQMALILTFAGSSQ
                     VVKIGRVAGQFAKPRSSPTEKKDGQELPSYRGDIINGPEFTAEDRIPDPRRQLEAYRQ
                     S"
BASE COUNT         1841 a         3712 c         3821 g         1904 t
ORIGIN      
        1 tcccgccgaa gcgcgcgagc agatgatggc ccgcgactgg ccggcgcggg acatcgggcc
       61 gctggtcgat ttgattgccg acccgcggca tcgcctggca gacacgggca ccatccggct
      121 cagcgatgag caggcacgtg ccattctcga cctgcgcctg cagcggctga ccgccctggg
      181 gcgtgacgag atcggcgatg agctcaagaa gctcggcgag gagatcaaga actatctggc
      241 cattctggcc tcgcgcgcgc gcatcgtcgg cctgatcaag gacgagctga ccgcgatcaa
      301 gacggagttc gccaccccgc gcctcaccga aatcgtggag atggaaggcg agctcgagga
      361 tgaggatctg atccagcgcg aggacgtggt ggtgacggtc agccatcgcg gctacatcaa
      421 gcgcgtgccg ctttcgacct accgcgccca gcgccgcggc ggtaagggca gggccggcat
      481 gtcgacgcgc gaggaggatg tggtcaccca gatcttcatc gcctccacgc acacgccggt
      541 gttgttcttt tcctcgcgcg gcatggtcta tcgcatgaag gtgtggcgcc tgccggcggc
      601 gacgccacag tccgtgggca aggcgctcgt caatctgttg ccgctcagcg aaggtgaaag
      661 catcacctcg atcctgccgc tgccggagga ggagaagacc tggggcagcc tggagctgat
      721 gtttgctacc cgcagtggca gcgtccgccg caacagtctc gccgatttcg agaatatcaa
      781 ccgcaacggc aagatcgcca tgaagctcga ggagggcgat cgcatcgtgc gcgtggccat
      841 ctgcacgccg tcggacaacg tgctgctgac gacggcgggt gggcgctgca tccgcttccc
      901 ggtcgaagac gtgcgcctct tcaaggggcg cgactcctcg ggcgtgcggg gcattcgcct
      961 cgaggacgac gacgaggtga tctcgatggc gatcctgcgc cacgtcgagg caagcccggg
     1021 cgagcgggcc gcctacatca agcaggccct ggccgtgcgc cgcgcccagg gggccgagat
     1081 tgccgatgcc acggtcgagc ccgcggccga ggtggaggcg gaagaaggcg aagaggccgt
     1141 cgatttgagc ccggaccgct atgccgagct gggcgcgcgg gagcagttcg tgctgaccgt
     1201 gtcggagcgc ggctttggca agcgctcgtc gtcctatgag taccgcatct ccggacgcgg
     1261 cggcaaaggc atcaccgcca tgatcgtcaa cgagcgcaac ggacgtctcg tggcgtcctt
     1321 cccggtcgag gaaagcgacc agatcatgct ggtgacggac ggcggcaagc tgatccgctg
     1381 ccccgtcgac gatgtgcgca ttgccggccg caacgtgcag ggggtgcgca tcttccgcac
     1441 cgacgatgac gaaaaagtcg tctcggtcga gcgcatctcc gaggaaggca atggcgatgg
     1501 cgcggccaac ggcggcgagg aggccgaacc caccgcctga ggacgccgcc gcgccgcgct
     1561 gacccgtcgg gtgggcgcga acgcatgccc gcgcaatggc gtggggagcc cgacaaatgg
     1621 gtgggcgcgg gttgaccgcg cgcccccaag cccgtccgcc tacttggcgg tcttcatggc
     1681 cccgaacgag aacacaaaat cctgcttgtc gagcggcttg tggcaggtga agcagttctt
     1741 caggttggcc ttgtcgttga cggtcttgtc ggccttgaag gcctggtatt cccattcgcc
     1801 gttgcgctgc tcgtcggggt attcgctgcc ccagccggtg cgcttttcca tcacggtgta
     1861 gccgatgagc tcgcccttga tgaagcggcc gttggcgtcc ttctcgggat tgccgtcggc
     1921 gtcgagtttg gccttgtgct gcaccagggt caggacgctg ccgcttggga tgggctgccc
     1981 ggccttgacc gcggcgatgg cttcgggcgt cgcatagagc tcccgatact gcttgttgtc
     2041 ggcgcggtcg acggtggtgt agagcacgcc cttggcatag ttctctggaa aagcgacctt
     2101 ttcgccgccg gcgcgcacgc ccacggttag cgtgacggcg gcaagcgcca gtgtggcggc
     2161 aatcggtaga gctttcataa tgtctcctcc cctccattag accttccgcc tacagcatac
     2221 ggtgccttgg cgggttcgga tcatgccagc ccatgaaatt tacgtgaggg ggcaacgccg
     2281 gcgccgcttg cggcggccgc aacttccgat tagggctttg ccgcacctgg catattcgcg
     2341 tgcccccgtt tgggaaagag cgtcgaccat gactgtggaa gtgctgttcg ccttcatcgc
     2401 tgcctgcctg ctgttgggcc tgacgccggg accgaacatg tcgctgatca tcgccaatac
     2461 gctgagcacc ggcctcacgg cagggctttt tacgctggcc ggcaccacca ccggcttggc
     2521 gttgctggtt ggcgccgccg tgatgggcat gagctcggtc atggtcttca tgtccgaatg
     2581 gttcgatgtg ctgcgctttc tcggcgcgct ctacctggtt tggctcggag cccgccagct
     2641 ctggcagttt tggtttgccg cgcccggcgc ggctgcgccg cccgcctcgc gcggcaactg
     2701 ctacgcacaa ggcttgctcg tcagcctgtc gaaccccaag gtgatgctct tccttggcgc
     2761 cttcctgccc cagttcatcg atccgggcgc ggacgcggtt gggcagctga ccttgctcgc
     2821 ggtcctgttc gtcgtggtgt tggcggcggt cgatgtgggc tatacgctgc tcctggcacg
     2881 ggcccgcacg cgctttagca tggcccgcct cagatggctc gatggggccg ccggggtgct
     2941 gctgctgctc gggggcgccg tgctggcgac catgcgacgc ccatgacccc tttgcgcctc
     3001 tttctcaagc gtgccatcgc cggctggggc ccgcgcgagg cagcgcgggc tcgctgcggg
     3061 gctcgggcgc agcggcacct caacgcgggc aagcggcgag cttgggggag cgatcgacga
     3121 tgaagcgcat tggctttacc tcgggctcct tcgatcccgt caccaacggg caccgcgacg
     3181 tgatcgagcg cgccgcgcgc ctggtcgata ccttggtggt cggtatcggg gtgcatcccg
     3241 gcaagacgcc gcttttctcg gtggccgaga agatcgagct gctcaaggcc gagacggcgc
     3301 cgatcgccaa ggccacgggc agccgaatag agatcgtcac cttcgacaac ttgacggtgg
     3361 aggccgctgc cgccgccggc gccggcgtca tctttcgtgg tctgcgcgat gccagcgact
     3421 tcgactacga gatgcagatg gccggtatga acggcagcat ggcccccgcc atccagacgg
     3481 tgtttctccc cgcgagcccc caggtccggc acattacggg aaccctggtg cgccagatcg
     3541 cgctgatggg cggcgatgtc tccaagttcg tctccgcagg cgtggcgcgc aaactcaagg
     3601 ccaaggcgcg agcgaaacaa gaaatcgtga agggctgagg caaacggtcc ggccggcagc
     3661 ggcagcgcgt gcaaagtggt tcccttgccg gccgtcctgc cctagaaggg ccacaagggc
     3721 ggcatcattc gcccgcagct cgctgccagg ttcggcaacg acgatgaccc tccttcaacc
     3781 ctaacgggag ccctctgtga tccgcttgtt gctgggcttt gccctcttcc tgttcgcctc
     3841 ggtttcgctc ctgcaggccc aagcgaccga tccgcagaat acgctggtga tcgaaaccac
     3901 caagggcccg atccgcatca agctgcgccc cgacctggca cccaagcatg tcgcgcagat
     3961 caaacagctc gccaagcagg gcttctacga cggcgtcatc ttccatcgcg tcattgccgg
     4021 cttcatggcc cagaccggcg atcccacggg caccggccgc ggcggctcca agctgcccga
     4081 cgtgccggcg gaatttggca gcgagccgtt caagcgcggc agcgtcgggg cggcgcgcag
     4141 ctctaatccc aatagcgcca acagccagtt cttcatctgc ttcggcgatg gctgccgcca
     4201 gctcaccgga cagtatacgc tgtggggcga agtgatcgac ggcatggaca acgtcgacaa
     4261 aattgcgcgc ggcgagccgc caccccaacc cgacaagatg cagaaggttt acctgcaggc
     4321 ggacaagaag tgacgcgctt ttccccgcgc gcgagtgcca gttgaagaga cagtgcggag
     4381 ttgccaatga cgcttgagca cgatccggaa aacacgctga tcatggagac caccaagggc
     4441 aaggtcgtga tcgggctcag gcccgatctg gcgcccaagc acgtggagcg catcaaaaag
     4501 ctggtgcgtg agggcttcta cgacggcgtc gcctttcacc gcgtgatcga cggcttcatg
     4561 gcccagaccg gctgccccga gggcacggga atgggcgggt cgaactaccc ggacctgaag
     4621 gcggagttca acgccgaaaa gcacgtgcgc ggcgtctgtt ccatggcgcg ctcgacgcgg
     4681 cccgacagtg ccaatagcca gttcttcatc tgctttgacg atgccacctt cctcgacaag
     4741 aagtacacgg tctggggaaa ggtgctcgaa ggcatggaga acgtcgacaa gctcgagcgc
     4801 ggcgaacccg tagcaaaacc ggataaaatc cgttccctga aggtcgccgc cgacgcttaa
     4861 aggctgaaac gccaagcgcc ggcgttaagc atggcctgcg cccgcgcggc ggctttcagc
     4921 tttcgtcaac cttgttttgc ttgagttcat ccggcacacg cttggcgggg tgtgcggctg
     4981 cgcccttgcg gcggggcgct ccgaggggtg ctcgcgtgcg ctacttcatg agcttctggc
     5041 tgggcttcgc gctggccgcc ggcctttggc tggcgagcgg ctggcgcggc gacggcgcgc
     5101 atgcggattt taccgacctc gccggcgtcg aagactggat ggcgcactac catctcaagc
     5161 ccgaaccgag gcgcttgccg caggcgatcg agacactggc ccgcgcgggt gcgctcgagg
     5221 gcgaggcgcg cctgaagccg gtggccgcat tcgtggccgc gctgatcggt gaggacgatg
     5281 ccacgctcgc ccgcctcagc gaggcgatcg cggaggcgcg cccggccaaa cagcggctgt
     5341 tggcagaggc gatcgccttg tcgggccggc cgcaatggcg ccgcctgctg accgagctca
     5401 aacgcgccgt gccggcccgc gcgctcgaga tcgaaacgct gttggccgcg ccggaggcgc
     5461 ccgtcacgct cgccgccgcc ttcgacgacg ccggcgtggt cgtcgacatg gtgtttgcgc
     5521 actttatggc gacgggaagc gaggcggcgt tccagcgcat ccttgccgcg ctcgccggtc
     5581 actttgcggc ggacgacccg gccgcagcga gcagcgcgga gaaagccaag gtgacgctgt
     5641 cgagccgggt tggcgacgat ccgcggctat tggagctggt acgtggggaa gccgaggcgg
     5701 aggacgagcc gctggcacgg ctattgcgcg gcgtccttgt cgccgccgcg caacaggcgc
     5761 gagggcgccc aagcggcaat tgagcgctca aagcgccagg ccttttgctc tgagcttgcc
     5821 ctataatcgc ctgatgcgag ccgaactctt cgatttcgag ttgcccgagg acctgatcgc
     5881 gctcgaaccc gccgagccgc gcgatgccgc ccggttgctg gtcgtgtgcc ccgatgccgg
     5941 ggtggccgct gcgccgaccc ccgcgcccgc ctcgccgccc gagcagcggg gagcgctgcg
     6001 ccttaccgac acgcgcctcg agcgattggc cgacctgctg cgtccgggcg acgccctcgt
     6061 cctcaacgat acgcgcgtga tccgtgctgc gctcaggggt gagcgcctgc gcggggagca
     6121 gcacgcggtc gtttccttca acttgcacaa gcgtttggac gaaagccgct ggctcgcctt
     6181 cgcgcgcccg gccaagcggt tgcgccgcgg cgaccgcatc cgcttcggtc acgacggccg
     6241 cgtgtgcctc ttgggtcagc tcgatgcgac ggtcacggcg ctgggtgatg ccggggagct
     6301 ggagctctcc ttcagcctgc atgggcccta tcttgacgag gccatcgagg cgctcggcga
     6361 cccgcccctg ccgccctaca tcgccagccg ccgcggttac gtcccggagg atgccgagcg
     6421 ctaccaaacc gtctatgccc gccacgccgg ggcggtggcg gcgccgacgg cgggtctgca
     6481 ttttacgccg gcggtgttcg ccgccctgga agcgcgcggc attagccggc atttcctgac
     6541 gctgcacgtc ggcgccggca cttttttgcc ggtgaaggcg gacgacacga cggatcatcg
     6601 cattgaggcg gagtgggggc agatcagcgc cgacagtgcg cgcgcgctca atgccgtacg
     6661 cgccgccggc gggcgcatcg ttgcggtcgg cagcacggcg ctccggctcc tggaaacggc
     6721 cgccgatcgg gacggcaggc ttggtgcctt cagcggcgag acacagctct tcattacacc
     6781 cggctatcgc ttcaaggcgg tcgatctgct gctgacgaac ttccacctgc cgcgctcgac
     6841 gctgttcatg ctggtggcgg ccttctcggg gctcgagaac atgcacgcgg cttatcggca
     6901 cgccatccag gcgcgttatc gcttctattc ttatggcgat gcctgcctgc tttatcccgc
     6961 cgacgccagc cgatgacccc gttttccttc cagctgcgcc acaccgatgc cggcgcccgc
     7021 ctcggcgaga tcaccaccgc acgcggggtc atcgccaccc cggcgttcat gcccgttggg
     7081 actgcgggca cggtgaaggc gatttacacc gaccagctca aggctgcggg cgtcgacgtc
     7141 gtgctcgcca atacctatca cctgatgctg cggcccgggg cggagcgcat cgcccggctc
     7201 ggtggactgc acacattcat gcgctgggag cggccgatcc tgacggactc cggtggtttt
     7261 caggtgatga gcctggcgaa gctgcgcaag atcaccgagg agggtgtcgc cttccagtcg
     7321 catatcgacg gcgcccgcca cctgctgacc ccggagcggg cggtggagat ccagtgcctg
     7381 ttgggctcgg atatccagat gcaactcgac gaatgcgtgg gtctgcccgc ctcccatgac
     7441 gccctcgctg tcgcggtcga gcgctccttg gcgtgggccc gccgggcaca gcgcgcgttc
     7501 ctcgccaagc gggcggacgg gcgagcccac ctcggccagg agttgttcgg catcgtccag
     7561 ggtggaaccc atcgcgattt gcgcgcgcgc tccgtcgatg gcctgatcga tcttggcttt
     7621 gcgggctacg cggtgggcgg cctggcggtc ggcgagccgc agcaggccat gctggaaacc
     7681 ctcgagggca cgctgccacg cctaccggcg gagaagccgc gttacttgat gggggtcggc
     7741 accccgctcg atctgatcga ggccgtcgcg cgcggcgtcg atatgttcga ttgtgtcctg
     7801 ccgacccggt ctggccgcca cggccaggcc ttcacctggg gcggcaagct caacctgcgc
     7861 aatgccgtca acagcgagga tcgcgccccg ctcgatgcat cgagccaatg ccccgccgcg
     7921 cgcgactatt cgcgcgccta cctgcatcac ctagtcaagg ccgaggaata tctggccgcc
     7981 atgctgctgt cctgggccaa tgtcgctttt tatcaggagc tgatggcggc catgcgccag
     8041 gccatcgccg aaggccgctt cgccgcctgg gccgccgcca ccaaggcggc gctcgcctaa
     8101 gcgcccatag cgcagatcgg aaaaagcctc ggcgtcgccg cgcttttttg caacagagga
     8161 acttgccacg ccgggccggc gttgtgggca ggggcttagc agggacggcg ccgatgtccg
     8221 cggagcagca gggccgaaaa gcaaagtggc gcgcccttgc cgcggaagcg cgcgcctgcg
     8281 cggccgagac aagcgaaccg cgggcgcggc ggatcctgca ggagatcgcc gcggcctacg
     8341 aacgcctcgc catcggcgag ccgccgcaga aacggccgag ctgaggcgca gcgcgccccg
     8401 ctctcgagcc cgggccctgc cgcgccgatg cgcagccgcc gcagccgccg cagccgcctg
     8461 tgcggtcaga tgcggcttag gccaccgtct cctcgatcag atgatcgacg acgctcctca
     8521 gcgcctcctc gcgcgtcgcc cccgccgcca cgcgcgccaa aaagtgttta acctgcctgt
     8581 cggcgctggt gccgcgcgcc agaatcgtgc gggcgtgctc gaggtcggcg ctgcagccaa
     8641 gcgccgcggc atcctctgcc agcatcacca acagctcatc gacgagatcg cggaaagcga
     8701 cgagctcacc cttgccgaga tcgaacaggg ttccctcgac gccatagcgc tgcgcgcgcc
     8761 agcggttctc ggcgagcagg aacaggggat aggtgcgcca ggtctgattg gcgcggcgca
     8821 accggtagag catgcgcagg atgcagagat agagcgcggc gacgcacacc gcatcatcaa
     8881 tcagtgtgca gacgtcagtg acgcgcatct ccagggttgg aaagcgcgag gagggtcgga
     8941 tgtcccacca gatcttgctg gcgtcctcga tgatgccgtt gcggaccagc aggtcgacgg
     9001 tctgcaggta ttcataccag ccctcgaagc ggcccggcat gcccgtgcgc ggcagctcct
     9061 ggtaggcggc gagccgatag ctcttgaggc cggtgtcctc gccctcagcg aacggggaag
     9121 aggtcgataa catcagcaaa tgcgggagga agtagcgcgc ctgattgaaa agatcgatgc
     9181 gcaactcgtc gtcttcgatg ccgacgtgca cgtgcatgcc gcagatcacc aggcgacgac
     9241 cgaccccggc caggtccttg gcgatcgcct ggtaacgcgc gcgctcggtg ggctcttgcg
     9301 tgctccagcg cgcaaagggg tgggtcgagg ccgcaatcgg cgccagacca tgctcggcgg
     9361 cgagcccagc caccgtgcgg cgcaagtggg caagctccgc gcccgcttgg cgcggggtgt
     9421 ggtgcacgct ggtgccgacc tcgatctggg atttcaggaa ctcgcgcgcc acttggccct
     9481 gcattgcctc ttgcgcggcc tcgaacaggg cctggggcat ctcgcgcacg agatcacggg
     9541 tcgtccgctc gacgaggagg tattcctcct cgatgccgat gctgaaggaa ggatccttga
     9601 gggccatcgg gaaatgttgg cacgaattgg gcggccttca aatcgcggcc ccccgcccgg
     9661 aggcgcaaat ttggggatgg cggggaccgc ggccaaaggg gctcgcccgc tttaggccgg
     9721 cgcgccagcc tcgagccagg ccgcggccgc gggccagagc gtcgtgcgga aggtcgggcg
     9781 gaagaagccc agatgcccga ttttcgtcgc ctgcgcgtcg gccaggcgaa cggaccagat
     9841 gctgcgttcg ctgttggcct tgaaatggtt ggccatatgc ccgacggcgg cgggcgtgcc
     9901 ccagacgtca tcctcgatct gggcaaaacg cacaggtgcc ctcagcgtgg cgaaatggcg
     9961 gacctcggga agcgtcggat cgccgaaaat gaagtccggc gtcatacacc agccggccca
    10021 ttccagaaat gccgggcccg gcatgttctc gccgcccatg aagacgccgg gaaagtagcc
    10081 aagcgcgcgc accaggggtg gggcgacgat ccccatcaga acgcgcacgc gatagcgttc
    10141 cgggctccgc atgcggcgcc aatagccgct gagcgtggcg atgttgagtt ggcgggcgat
    10201 ggcggcgttg ttatgggcga ggcccggcgc aaagccgccc atgctgtgac ctagccaatg
    10261 caagggccgc tcggggtggg cgcgccccgc ccaagcaatc acgccgggga cgtcgaggat
    10321 acaccagtcg cgcatgcggc cggcaaagcc ccgcaagctg gggggtgagg aggcgccgat
    10381 gccgcggtaa tcgaagctca cgaccagccc gccgcgttcg gccagatagc tggcgaaccg
    10441 cgcgtagtag cgggcgggta cggcggcggc ggggctgatg accgtgatcg gggcccgttc
    10501 ggctgcggca ccggactgga acagggtcgc cgccagggaa tagccgtctg ccgcggtaat
    10561 gctgaacggg ctgaccgtca cggcggccgc atcgtccggg gcgctcattg catctttgcc
    10621 attaacgctg tagcgcccgg cgcgcgacct gggccggaga aggtccgggc cccctggtgg
    10681 cgcctgtccc tttccactat aacgccggcg cagaccacat ttgtcttgat ctgccttgac
    10741 cgcccgcgac ggttgtcgca ggcgcttttg tcgattttgg ctggagaatt cgtgatgttg
    10801 gccgcatgga agcccgatag ctggagatcg aagccgatcg tccaggtccc agagtacccc
    10861 gatcccctag ccttggccga tgttgagcac aatttgcgca attttccgcc cctggttttt
    10921 gtcaaggaag tgcgcgatct caaggagaag ctcgctggcg tcgccgaagg caaggccttt
    10981 ttgctgcagg gtggggattg cgcggaaagt ttcaaagagc acaagaccga ctacattcgc
    11041 gattatttcc agctcttcct gcaaatggcg ctgatcctga cgtttgccgg cagcagccag
    11101 gtcgtgaaga tcgggcgcgt cgccgggcag ttcgccaagc cgcgctcctc cccgaccgag
    11161 aagaaggacg gccaggagct gccgagctac cggggcgata ttatcaacgg tcccgagttc
    11221 acggccgagg accgtatccc cgatccgcgc cggcaactgg aggcctaccg gcaatcag
//
LOCUS       DASTMT010000039        12965 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_5588264, whole genome shotgun sequence.
ACCESSION   DASTMT010000039 DASTMT010000000
VERSION     DASTMT010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 12965)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12965)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12965
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5588264"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1114)
                     /locus_tag="VFR00_00855"
     CDS             complement(<1..1114)
                     /locus_tag="VFR00_00855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="double-strand break repair protein AddB"
                     /protein_id="HEU0157825.1"
                     /translation="MRAADLFSLPRSRHAAGRIFSVPHGRPFLRAVAEALLTGELPRP
                     GGRRFGALELAGVTLLLPTRRDIAAVQEAFLQAANGAALLLPKIKLISHMSDAPPLAA
                     AEGGERADGEGKPQIGKLERELALARLVLQWSRAIAASQGRAPTRTPAQAVRQAKELA
                     RLLDMVEMEDVGLTRLDALVPQDFSAHWELTLDFLKIVTEHWPRYLDDLGKVSPAAWR
                     NGLMLREAERLRGTPPNEPIIVAGVTGTVPAATQLMRAVLTLEQGALVLPGLDLTLDE
                     ESWNVLVPAHPEHPQFGLKKLLDALGVSRSEVVVLPGPSPTARQKARARLVSEAMRPA
                     STTERWHTFLEGSAAATLHEALAGCTILEAASAADEA"
     gene            complement(1101..1838)
                     /locus_tag="VFR00_00860"
     CDS             complement(1101..1838)
                     /locus_tag="VFR00_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEU0157826.1"
                     /translation="MTGAKAPLSIDTAMVLSAGLGTRMAALNGKLPKPLIGLNGKPLI
                     DHVLDRLAQAGVRRVVVNVHHYADLIEDHLKGRALPSISLSDERAQLLDTGGGVKKAL
                     PRLGPKPFLIHNADSVWIESIGSNLSRLVAAWEPTRMDCLLLLAPQSISPGYHGRGDF
                     ALEADGRIRRRGEQEVVPFVFAGVSIAHPRLFDASPEGAFSLNLLWSRAIAAGRAWGM
                     RMEGLWMHVGTPEALMQAEQCLNDASR"
     gene            complement(1850..3382)
                     /gene="tsaE"
                     /locus_tag="VFR00_00865"
     CDS             complement(1850..3382)
                     /gene="tsaE"
                     /locus_tag="VFR00_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEU0157827.1"
                     /translation="MSFYWQRELDEAGVVRLAELLALRLKRGSLVALSGELGVGKTTF
                     ARALIRALVAAEIEVPSPTFALQQGYATARLMLTHFDFYRLANAEEALELGFEEALAQ
                     GAVLAEWPERVAALLPTDRYEVALADAEASLRRRVTVTGCGALAGDVERLEAAFRLLE
                     REPATPLSRIRYLQGDASTRTYARLWQGNRTELLMDAPRQADGPPIRDGKSYSKIAHL
                     AEDMVRPFVAIAAVLVAQGLSAPRVLAADLEHGLALVEDLGDRLFGAEIAAGTAKALL
                     YRSAVDVLLRLRAVPVPPRLALPDGGSYALPRRDRAAFEIELELLIDWYWPALKDGPC
                     PPEGRAEFRELWRGILDQLLALPGAWFLRDFHSPNLIWLPEREGLARVGILDFQDALN
                     EHAAFDLVSLLQDARLDIPEALERELFEYYCAGALALDAAFDRSSFAGAYAMFGAQRN
                     TRLLGLWVRLLRRDGKPHYLQHIPRTWGYLERNLRHGAMADLAAWYARHFPPVLRVGL
                     PN"
     gene            complement(3379..5823)
                     /locus_tag="VFR00_00870"
     CDS             complement(3379..5823)
                     /locus_tag="VFR00_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU0157828.1"
                     /translation="MLLAAGILILAALLFGTVAGMQPLLAAGKSGALLLVSVAIGAAA
                     LAAAMLHRAVQRYAKSDQSLAAEITELRKSLLAAEAVIKAEPQVLVFWEQGQAVRIMM
                     HTLGGVAGLPAEPRQLLAFGSWLDAASAQQLKKGLDVLFAEGQSFTLLIKTVSGGYVE
                     ADGRAGGGRAILRLRAVAGRKQDLAKILDQHRQLVRDTIAGRTLLNALPMPAWFRGID
                     GRLEWVNEAYVKAVDAASGAEVRERQIELLETRQREAVKGALDKGVAYRDRVHLISEG
                     ARKAHDVVILPLDGAAVGAAIDVAALETAQGELVRHVAAYERTLDRVATGVAIFGPDQ
                     RLAFFNEAYRKLWLLDADWLASKPSDGEILDRLRELSRLPEVGNYRDWRSKILAGYRT
                     GGEYDDWWHLLDGRTIHVVSAQRPDGGLTYLYDDVTERFALESRYNALIDAQRETLDN
                     LKEGVAVFATDGRLKLFNSAFAQIWRLSRSTLGEGPHIDEIIAQCVGLYDDMPTWAGI
                     SRAVTGISDRRQPLAAQMTRSDQAVIDFAVSPLPDGATLITFVNVTDTKRYEQTLIER
                     NDALVAADRLKNQFISHVSYELRTPLTNIIGFGELLSSPRTGELNNKQREYLSDIAAS
                     SRSLLAIINDILDLATIDAGALELKLAPVKVAAVIDTAVLGIRDRANRARLNLDIRVA
                     DDAQEFIADESRVRQVLYNLLSNAIGFSKPGTTIGIKAWREAGMTAFMIEDSGVGIPK
                     EQQSRVLERFESRSQGSKHRGAGLGLSIVKSLVELHGGSMALDSEPGRGTRVTVRFPE
                     NGTEFGAPAAVWAADA"
     gene            complement(6074..6610)
                     /gene="mobB"
                     /locus_tag="VFR00_00875"
     CDS             complement(6074..6610)
                     /gene="mobB"
                     /locus_tag="VFR00_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007819175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HEU0157829.1"
                     /translation="MTQPSNPPVVGIAGWKKSGKTTLITGLIEEFTRRGLKVASVKHA
                     HHAFQIDAEETDSAKHRRAGAVRVAIVSGHRWALVNELRGAPEPSLETVLAALGSCDL
                     ILVEGYKAAAIPKIEARRGATRKQQPLAPDDANVIAIAADHTADGAGRPVFALDDVAG
                     LADFLALRLGLGAGSAKA"
     gene            complement(6607..7287)
                     /gene="mobA"
                     /locus_tag="VFR00_00880"
     CDS             complement(6607..7287)
                     /gene="mobA"
                     /locus_tag="VFR00_00880"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008836510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HEU0157830.1"
                     /translation="MRVKEVLPRLSIDESCMINAPVTGVLLAGGQSRRMGGGDKGLLR
                     LDGKSMLAHVIERLSPQVSAIVLNANGNPERFAELGLPVVADPVAGFVGPLAGVLAGM
                     RWSLAHAPERRFVVTAAGDAPLLPRDLVARLLAAAGAGRQIALARSNGMLHPVIGLWP
                     VALADDLEDALGQGVRKVLDWTDRHGTVGVDFANERIGAREIDPFFNANTPQEFDDLR
                     ALLASASA"
     gene            complement(7250..8131)
                     /gene="fdhD"
                     /locus_tag="VFR00_00885"
     CDS             complement(7250..8131)
                     /gene="fdhD"
                     /locus_tag="VFR00_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018688196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HEU0157831.1"
                     /translation="MQSFEIRPDPDDPRLSVTVSGIDHEGKRVETAVVTERPLTLFLN
                     AREIVTMMTIGDRPDLLAVGYLLNQNMLRADDEIAAVEYDDEIATVVVRTKRQTDFEE
                     KLKKKIQTSGCAQGTVFGDLMEDFDKVTLNAAARIKTSWLYELTKRINTAPSLYLKAG
                     AIHGCVLCQENRPLIYMEDVGRHNAVDKIAGHMFLNKIAADDKIFYTTGRLTSEMVIK
                     TVKMRIPILVSRSGFTAWGVELAQRAGLTLIGRARGKRFVALAGAERIVFDADLGKIA
                     EEDRRHARKGSVAEAVD"
     gene            8487..9587
                     /locus_tag="VFR00_00890"
     CDS             8487..9587
                     /locus_tag="VFR00_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020011921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEU0157832.1"
                     /translation="MDRRKFLAGAGMAGGAGLLSGLPAPAIAQSAPELKWRLTASWPK
                     SLDTLYGGAEHFAKYVAEATDNKFQIQTFAAGEIVPGLQATDAVASGTVEMCHTAAYY
                     LWGKDPTFALSCAVPFGLNPRMQNAWWTEGGEALVNAFYAKHNIVGLLAGNTGAQMGG
                     WFRKELNTPDDLKGVKMRIAGFAGAVMAKVGVVPQQIAGGDIYPALEKGTIDAAEWVG
                     PYDDEKLGFYKVAKFYYYPGWWEGGAMLHVHVNKAKWDELPATYKSILKSAAADANIF
                     TLGRYDSQNPQALKRLVANGTQLRPFSEAILDVCFKSANEVYADISGKNEDFKKIWDS
                     IRAFRSDQYLWLQLADNTYDTYMMIQQRKKTL"
     gene            9900..11015
                     /gene="apbC"
                     /locus_tag="VFR00_00895"
                     /pseudo
     CDS             9900..11015
                     /gene="apbC"
                     /locus_tag="VFR00_00895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015314115.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
     gene            11028..11393
                     /locus_tag="VFR00_00900"
     CDS             11028..11393
                     /locus_tag="VFR00_00900"
                     /inference="COORDINATES: protein motif:HMM:NF017922.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HEU0157833.1"
                     /translation="MSLARRLEPPKLSISPDRGALEVAFDGAAPVSLSAEMLRVMSPS
                     AEVQGHSPDQRVTVARKRNVKIKELKPVGNYAARIVFDDGHDTGLYSWAYLRTLTEER
                     EKRWAEYLSALAQKGMSRD"
     gene            complement(11390..12346)
                     /gene="speE"
                     /locus_tag="VFR00_00905"
     CDS             complement(11390..12346)
                     /gene="speE"
                     /locus_tag="VFR00_00905"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013419603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HEU0157834.1"
                     /translation="MDRWIEETFHPHWRVRLEASRVLHEVKTEHQHLVIFENPTWGTV
                     LMLDGVCQLTTSDEFVYHEMMAHVPLMALERPKRVLIIGGGDGGVLREVLKHPSVLRA
                     TLCEIDRTVIDTARQHYPSIPGAAFEDLRTDVVIADGCRYVAETDERFDAIIVDSSEP
                     IGPSAVLHTREFFQDCRRALKRGGVLVTQNGLPFLFPAHLGGTTAVFASLFKRVAPYL
                     CTQPCYFGGPFALNLATDDKHVLKVEAKQLAKRQKKRGVRGLKYWTPHVHVGAFALPA
                     YAQRVVDAAIDEARAQSTPSAGYVAVATAAPAEAALAKSTDA"
     gene            complement(12446..12964)
                     /gene="speD"
                     /locus_tag="VFR00_00910"
     CDS             complement(12446..12964)
                     /gene="speD"
                     /locus_tag="VFR00_00910"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013419601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HEU0157835.1"
                     /translation="MDLTRSSTAQKEDHGQTALGAHDDRKDYFIERDGVRFAGTHLLI
                     DLFGAKRLDDLKHIKETLKRCVEVAGATLLHIHLHHFTPNGGVSGVAVLAESHISIHS
                     WPEVGYAALDVFMCGHADPHATIAVLETAFRPDRTVVKEHLRANSASERTWEIATAKK
                     TAARSTRAKKAA"
BASE COUNT         2216 a         4272 c         4314 g         2163 t
ORIGIN      
        1 cggcctcgtc cgccgccgaa gccgcttcaa ggatggtgca gcccgccagc gcctcatgca
       61 gcgtcgcggc ggcgctgccc tccaggaagg tgtgccagcg ctctgtggtg ctggcgggcc
      121 gcatcgcctc gctcaccaag cgcgcgcggg ctttttgccg cgctgtcggt gacgggcccg
      181 gcagcacgac cacctcgctg cggctgacgc ccaaggcatc gagcaatttt ttcaggccga
      241 actggggatg ctcggggtgc gcgggcacaa gtacattcca gctctcctca tcgagcgtca
      301 gatcgagccc cggcaagacg agggcccctt gctcgagcgt caacaccgcg cgcatcagct
      361 gggtggccgc cggcacggtg cccgtcacgc cggcaacgat gatgggttcg ttcggcgggg
      421 tgcccctcag gcgctcggct tcccgcagca tcaagccgtt acgccaggcg gccggcgaca
      481 ccttgcccag gtcgtcgagg taccgcggcc agtgctccgt cacgatcttg aggaagtcga
      541 gcgtcaactc ccaatgggcg gaaaagtctt gcggcacgag ggcgtcgagc cgcgtgaggc
      601 cgacgtcctc catttccacc atgtcgagca ggcgcgccag ctctttggct tgccgaacgg
      661 cctgcgccgg cgtgcgcgtc ggcgcgcgtc cctggcttgc cgcaatcgcg cgggaccact
      721 gcagcaccaa gcgggcgagc gcgagctccc gctcgagctt gccgatctgt ggcttgcctt
      781 ccccgtccgc gcgctcgccc ccttcggccg cggcgagcgg gggcgcgtcg ctcatgtgcg
      841 aaatcagctt gatcttgggg aggagcaaag ccgcgccgtt ggcggcttgc aggaacgcct
      901 cctgcacggc ggcgatgtcg cgccgtgtgg gcagcagcaa ggtgacgccg gcgagctcca
      961 gggccccgaa gcgtcgcccc cccggcctcg gcagctcgcc cgtcagcagg gcctcggcga
     1021 cggcgcgcag aaacgggcgg ccgtgcggta cgctaaagat gcgcccggcg gcatgccgcg
     1081 agcgcggcaa cgaaaagagg tcagcggctc gcatcgttca ggcactgttc cgcctgcatc
     1141 agagcctcgg gcgtaccgac gtgcatccat agcccctcca tgcgcatccc ccacgcgcgc
     1201 ccggcggcga tcgcgcggct ccacaggaga ttgagggaga aagcgccctc tgggctggcg
     1261 tcgaacaggc gtggatgcgc gatggaaaca ccggcgaaca cgaacggcac cacctcctgc
     1321 tcgccgcggc ggcgaatgcg gccgtcggcc tcgagcgcga aatcgccgcg gccgtggtag
     1381 cccgggctga tcgattgcgg tgccagcagc aataaacagt ccatgcgcgt tggctcccaa
     1441 gcggcgacga ggcgggagag attggagccg atgctttcga tccacaccga gtcggcattg
     1501 tgaatcaaga acggcttcgg cccgagccgc ggcagggcct tcttgacgcc accaccggtg
     1561 tcgagcagct gcgcgcgctc atcggagagg ctgatgctgg gcaacgctcg gcctttgagg
     1621 tgatcttcga tgagatcggc gtagtggtgc acgttgacga ccacccggcg cacgccggct
     1681 tgggcgaggc gatcgagcac gtgatcgatc agcggcttgc cattgaggcc gatcaagggt
     1741 ttgggtagct tgccgttcag cgcggccatg cgggtgccga gacccgcgct caagaccatg
     1801 gccgtgtcga tgctgagcgg cgctttggcc ccggtcatcc tctgcctcgt tagttcggca
     1861 acccgacgcg caggacgggc gggaagtgcc gagcatacca cgccgccagg tccgccatcg
     1921 ccccgtggcg gagattgcgc tcaagatatc cccatgtgcg tggaatgtgt tgcaggtaat
     1981 ggggtttgcc gtcacgccgc aacaggcgca cccagaggcc gaggagccgc gtgttgcgct
     2041 gtgcgccgaa catggcatag gcgccggcaa agctgctgcg atcgaaggcg gcatcgagcg
     2101 ccagggcgcc ggcgcaatag tactcgaaga gctcgcgctc gagcgcttcg ggaatatcga
     2161 gccgcgcgtc ctgcaagagg gaaacgagat cgaaggcggc atgctcgtta agcgcatcct
     2221 gaaagtcgag gatgccaacc cgggcgaggc cttcccgttc gggcagccaa atcaggttgg
     2281 gagagtggaa gtcgcgcagg aaccaagctc ccggcagcgc cagcagctga tccaggatcc
     2341 cgcgccaaag ctcgcgaaac tccgcgcgcc cttccggcgg acaaggtccg tccttgagcg
     2401 cgggccaata ccagtcgatg agcagctcaa gttcgatctc gaaggcggcc cgatcgcgcc
     2461 ggggcagcgc gtagctgccg ccgtcgggca gcgccagccg gggtggcact ggcaccgcgc
     2521 gcaaacgcaa gagcacgtcg acggcactgc gatagagcag ggccttggcg gtgccggcgg
     2581 caatctcggc gccaaacaag cgatcgccca agtcctcgac cagcgccaga ccatgctcga
     2641 ggtcggcggc gagcacccgc ggcgcgctga gcccctgcgc gaccagcacg gcggcgatgg
     2701 ccacgaaagg gcgcaccatg tcctcggcca agtgcgcaat tttgctatag gacttgccgt
     2761 ctcggatggg tgggccgtcg gcttgccgcg gcgcgtccat caagagctcg gttctattcc
     2821 cctgccacag gcgggcatag gttctggtcg aggcgtcccc ttgcagatag cgaatgcgcg
     2881 acagcggcgt tgccggctcc cgctcgagca acctgaaggc ggcctcgaga cgctccacat
     2941 cgccggccag ggcgccgcag ccggtgacgg tgacgcgccg cctcaggctc gcctcggcat
     3001 cggcgagcgc tacctcgtag cgatcggtcg gcagcagcgc ggccacgcgt tcgggccact
     3061 cggccagaac ggccccctgg gccaaggcct cctcgaagcc gagctcgagc gcctcctccg
     3121 cattcgccag gcgataaaaa tcgaaatgcg tcagcatcag ccgcgctgtg gcatagccct
     3181 gctgcaaggc aaaggtgggg ctcggcacct cgatctcggc agcaacgagc gcccggatca
     3241 aggcgcgcgc gaacgtggtc ttgccgaccc cgagctcgcc gctgagcgca acgaggctgc
     3301 cgcgcttcag ccgcagcgcc aacagctcgg ccagcctgac gacgccagcc tcgtcaagct
     3361 cacgctgcca ataaaagctc atgcatccgc ggcccagact gccgcaggcg cgccgaactc
     3421 ggtgccgttc tcgggaaagc gcaccgttac gcgcgtaccg cggcctggct ccgaatcgag
     3481 tgccatgctg ccaccatgca gctccaccag gctcttgacg atggagagcc cgagccccgc
     3541 gccacgatgc ttggagccct ggctgcggct ttcgaaacgc tcgagcaccc ggctctgctg
     3601 ttcctttggt atgcccacgc cgctgtcctc gatcataaag gccgtcatcc cggcttcgcg
     3661 ccaggccttg atgccgatcg tggtgcccgg cttggagaag ccgatcgcat tcgacaatag
     3721 gttgtaaagg acctgccgta cacggctttc atcggcgatg aactcctggg catcgtcggc
     3781 aacgcgaatg tcgagattga ggcgggcgcg gttggcgcga tcacgaatgc cgagcaccgc
     3841 cgtgtcgatt acggccgcga ccttgacggg ggccagcttc agttccaggg cgcccgcgtc
     3901 gatggtagca aggtccagga tatcgttgat aatcgccaac agcgagcgcg aggaggcggc
     3961 gatgtcactc aaatattcgc gttgcttgtt gttgagctcg ccagtgcgcg ggctggaaag
     4021 cagttcgcca aaaccgatga tgttggtgag cggcgtgcgc aactcgtagg agacgtggct
     4081 gatgaattgg tttttcaacc gatcggctgc caccagcgcg tcgttgcgct cgatcagcgt
     4141 ctgctcgtag cgcttggtgt cggtgacgtt gacgaaggtg atgagcgtgg cgccgtcggg
     4201 tagcggcgac acggcaaagt cgatgacggc ttgatccgag cgcgtcattt gcgcggccag
     4261 cggttgccgg cgatccgaga tcccggtcac ggcgcgactg atgccggccc aggtcggcat
     4321 atcgtcatag aggccgacgc actgggcgat gatctcgtcg atgtgcgggc cctcgccgag
     4381 cgtgctgcgc gagaggcgcc agatctgcgc gaacgccgaa ttgaagagct tgaggcgtcc
     4441 gtcggtggcg aagacggcca cgccttcctt gaggttgtcg agggtttcgc gctgcgcatc
     4501 gatcagcgcg ttatagcggc tctcgagcgc gaagcgctcg gtgacatcgt cgtaaaggta
     4561 ggtcagtccg ccatcgggcc gctgggccga gaccacgtga atggtgcggc catcgagcag
     4621 atgccaccaa tcatcgtact ccccgccggt gcggtagccg gccaggatct tggagcgcca
     4681 atcgcggtag ttgccgacct cgggcagccg cgacagctcg cgcagccggt cgaggatttc
     4741 cccgtcgctc ggcttgctgg ccagccaatc ggcgtcgagc agccacagct tgcggtaagc
     4801 ctcgttgaag aacgcaagcc gctgatcggg gccgaagatg gcgacgccgg tggccacccg
     4861 atcgagcgtg cgctcgtagg cggcgacatg acgcacgagc tcgccctgcg ccgtttccaa
     4921 ggccgccacg tcgatggcgg cacccacggc ggcgccgtcg agcggcagaa tgacgacgtc
     4981 gtgggccttg cgcgcaccct cgctgatgag gtgcacccgg tcgcggtagg ccacgccctt
     5041 gtcgagagcc ccttttaccg cttcgcgctg gcgcgtctcg agcagctcga tctggcgctc
     5101 gcggacttcc gccccgctgg cggcatcgac cgctttgaca taggcctcat tgacccattc
     5161 cagacggccg tcgatgccgc ggaaccaggc cggcatcggc agcgcgttga gaagggtgcg
     5221 cccggcgatg gtgtcgcgca cgagctggcg gtgctgatcg aggattttgg ccaagtcctg
     5281 cttgcggccg gcgacggcgc gcagacgcaa gatggcgcgc ccgccgccgg cgcggccgtc
     5341 ggcttcgacg tagccaccgc tcaccgtctt gatcaacaag gtgaaggatt gcccctcggc
     5401 aaacaggaca tcgaggcctt ttttgagctg ctgggccgag gcggcatcga gccagctgcc
     5461 aaaggccagg agttggcgtg gctcggcagg caggccggca acgccgccga gcgtgtgcat
     5521 catgatgcgg acggcttggc cctgctccca gaagacgaga acctgcggct cggccttgat
     5581 caccgcctcc gccgcgagca gacttttgcg cagttcggtg atctcggccg ccaggctttg
     5641 gtcgctcttg gcgtagcgct gcacggcgcg atgcaacatg gcggccgcca gtgcggccgc
     5701 gccgatggcc accgagacga gcagcagcgc gcccgatttg ccggccgcga gcaggggttg
     5761 catccccgcc acggtgccaa acagcagcgc cgcgaggatg agaataccgg ccgccaggag
     5821 cacgaccgtc tccccgcgcg gtctgccgct ctcaacctca gcgctcgccc gcgacatcaa
     5881 aaccgatcac agccggcaat acgccaaggg cgcacgcaca acgatggggc cggggcgcac
     5941 ggtgccgttc cctactgtta acgaatcatt aatgattatg tgggggttcg ccgcccatgg
     6001 ccatagcaga tggggcacag ggacgagccc gccgtaaggc tggcaaagga cgggcgcgga
     6061 tcggccggtc gccttaagcc ttggccgaac cggcgccgag ccccagccgc agcgccaaga
     6121 agtcggcgag cccggcgacg tcgtcgaggg cgaagaccgg gcggccggca ccgtcggcgg
     6181 tatggtcggc ggcaatcgcg atcacgttgg catcgtcagg cgcgagcggc tgttgcttgc
     6241 gcgttgcccc gcgccgggcc tcgatcttgg gaatggccgc agccttgtag ccctcaacca
     6301 ggatcagatc acaggacccg agcgcggcca gcacggtctc gaggctcggc tccggcgcac
     6361 cgcgcagctc gttgacgagg gcccagcgat ggcccgagac gatcgccaca cggacggcgc
     6421 cggcccgccg atgcttggcg ctgtcggtct cctcggcgtc gatttggaag gcatgatggg
     6481 cgtgtttgac cgaggccacc ttgagaccac gccgcgtgaa ttcctcgatc aacccggtga
     6541 tcagcgtcgt cttgccggat ttcttccatc cggcgatgcc gaccacgggg ggattggagg
     6601 gctgcgtcat gcgctggccg acgccaacag ggcgcgcaag tcgtcgaact cctgcggcgt
     6661 gttggcattg aagaagggat cgatttcgcg cgcgccaatc ctctcattgg caaagtcgac
     6721 gcccacggtg ccatgccgat ctgtccagtc gagcaccttg cgcacgccct ggcccagggc
     6781 gtcttccagg tcgtcggcca gcgcaaccgg ccacaggccg atcaccgggt gtagcatccc
     6841 attggagcgg gcaagcgcga tttgacgccc cgcgccggcg gccgccagca gtcgtgccac
     6901 caaatcgcgt ggcaggagcg gcgcatcgcc ggccgcggtg acaacgaagc gacgctctgg
     6961 ggcgtgcgcg agcgaccagc gcatgcctgc cagcacgccc gccagcggcc ccacgaaacc
     7021 cgcgacggga tcggcaacga cgggcaaacc gagctcagca aagcgctccg ggttgccgtt
     7081 ggcgttgagc acaattgcgc tcacttgcgg ggatagccgc tcgatcacgt gcgcaagcat
     7141 ggatttgcca tcgagccgca agaggccctt gtcgccacca cccatgcggc gcgattgtcc
     7201 ccccgccagc aggacgccgg tcaccggcgc gttgatcatg cagctctcgt caatcgacag
     7261 cctcggcaac acttccttta cgcgcatgcc ggcgatcttc ctcggcgatc ttgccgagat
     7321 cggcgtcgaa gacgatgcgc tccgccccgg cgagcgccac aaagcgcttg ccgcgggcgc
     7381 ggccgatcag ggtgagcccg gcccgttgcg ccagctcgac gccccaggcg gtaaaaccgg
     7441 agcgcgagac gagaatggga atgcgcatct tgaccgtctt gatcaccatc tcggaggtga
     7501 gacgcccggt ggtgtagaaa atcttgtcgt cggcggcgat tttgttgagg aacatgtggc
     7561 cggcgatctt gtcgacggcg ttgtggcgac cgacgtcctc catgtagatc agcggccggt
     7621 tctcctggca cagcacgcag ccatgaatcg cgccggcctt gagataaagg ctgggcgccg
     7681 tattgatgcg cttggtcagc tcataaagcc acgacgtctt gatccgagct gcggcgttga
     7741 gcgtgacctt gtcgaaatcc tccatcagat cgccgaacac ggtgccttgc gcgcagcctg
     7801 aggtttgtat cttctttttc agcttttctt cgaaatccgt ttgccgtttg gtgcgaacca
     7861 caaccgttgc aatctcgtca tcgtactcga ccgcagcgat ctcgtcgtcg gcgcgcaaca
     7921 tgttctggtt caacagatag ccgaccgcca acaggtccgg acgatcgccg atggtcatca
     7981 tcgttacgat ctcccgcgcg ttcaaaaaca gcgtcagcgg ccgctccgtg acgacggcgg
     8041 tctccacacg cttgccctcg tgatcgatcc cgctcacggt gaccgaaagt cggggatcgt
     8101 ccgggtccgg gcggatttca aagctctgca cccgcttgac ctctccttga agctggcacg
     8161 gcctatcgac agggatgtag ccacttcgcc gcgcccccgc aagcgccgct gccggcgcgg
     8221 ttcgccgtgt tggcgcggcg aatcaccatt gagcggacta acttctctcc ttatagtgcg
     8281 tggaccagcc tgcgcggctt cccaccacga ccagtctgcg gccccggctg gcgccggcga
     8341 aggtgcagga tcactgcgtt gctttcgatg ggtttggcgg cagccaccaa tcgcgatcgg
     8401 cgcctcagct gtagccgggc atttcgcttg cgggccgttc ggctcaaaca acccgttgta
     8461 agggaggtaa gtcccagatg cgaaccgtgg atcgtcgcaa attcttggca ggagccggga
     8521 tggccggcgg cgccgggctc ctcagcggcc tgcccgctcc ggccatcgcg cagtctgcgc
     8581 cggagctcaa gtggcggctc acggcgagct ggccgaagtc gctcgatacg ctctacggtg
     8641 gcgcggagca tttcgccaag tacgtagcgg aagcgaccga caacaagttt cagattcaaa
     8701 cctttgctgc cggcgaaatc gtgccgggcc tgcaggcaac cgacgccgtc gcctcgggca
     8761 ccgtggagat gtgccacacc gccgcctatt acctgtgggg caaggatccg acctttgcgc
     8821 tcagctgcgc ggtgcccttc gggctcaatc cgcgcatgca gaacgcgtgg tggacggaag
     8881 gcggcgaggc cctcgtcaat gctttttatg ccaagcacaa catcgtcggc ttgctggccg
     8941 gaaacaccgg ggcgcagatg ggtggctggt tccgcaagga actcaacacg cccgacgatc
     9001 tcaagggcgt caagatgcgc attgccggct ttgcgggtgc ggtgatggcc aaggtcggcg
     9061 tggtgcccca gcaaattgcc ggcggcgata tttatcccgc gctggagaag ggcaccattg
     9121 atgcggccga atgggtcggc ccctacgacg atgagaagct cggcttctac aaggtcgcca
     9181 agttctacta ttatcccggc tggtgggaag gcggcgccat gctgcacgtg cacgtcaaca
     9241 aggccaaatg ggacgaactg cccgccacct acaaaagcat tctgaagtcg gcggcggcgg
     9301 acgccaacat tttcacgctt ggccgctacg attcgcaaaa cccgcaggcc ttgaagcgat
     9361 tggtggccaa cggcacgcag ctgcgaccgt tctcggaggc gatcctcgac gtctgcttca
     9421 agtcggccaa cgaggtttat gccgatatca gcggcaagaa cgaggacttc aagaagatct
     9481 gggattcgat tcgcgccttc cgcagtgatc aatacctctg gctgcagctg gcggataaca
     9541 cctacgacac ctacatgatg atccagcaac gcaaaaagac cctgtgagct tgcggcgagg
     9601 ctgatcgaac ctggccgttt gccgcatgga ccggggctcg ggctgcgctg gagcccgaca
     9661 aggctgccgc gcccgagcaa accgcgtgcg ggcgcgcgcc gctcttcggc ggtgctccgt
     9721 gctcaagccg gcagtgctgg gcctggggtg ctggggccag cggtgctggc ggccttcggt
     9781 gacagggcgg gagactttgc cagggcctac cgccctgggc gcaaccccgg agcaaggggc
     9841 tcggagccaa cgccggcgtg cgccgtctag tcgcgcggga cttgtgaagg agaggatcaa
     9901 tgagcgtcag ccgggagcag gttttggagc gcctgcgccg cgtcaagggt cccgaccttg
     9961 agagcaacat cgtcgacctc ggcctcgtct ccgaggtcct cgtcaaggac ggccgggtat
    10021 cgttttccat taccgtcccc gccaagcggg gcgctgagct cgagccgttg cgccaggcgg
    10081 cggaaaagat cgtgcaggag atcgacggtg tccggcacgt gaccgccgtg ctgacggccg
    10141 aacgcgcacc ccgcgccggc ggccaggcgc cggagagcca gcgcgtgcgg gaggcgcgcg
    10201 ccaggggcgc cgcgcagagc ccgccggccc cgccgcctcc ccatgccgct cccggatcgc
    10261 gccagatcac ggaggtggcc ggcgtgcgcc acttgatcgc cgtggcgtcc ggcaaaggtg
    10321 gcgtcggcaa gtcgaccacg gcggtcaacc tggcgctcgg actgcagggg ctcggtctca
    10381 aggccggcat tctcgatgcc gatatctacg gtccctccca gccacgcctg ctcggactga
    10441 ccggccggcc gcaggtggcc gagggcaaga tgctgcggcc gatggaaggc ttcggcctca
    10501 aggccatgtc catgggcttc ctggtcgacg aggccacgcc cgtcatttgg cgtgggccca
    10561 tggtcgtctc ggcgctgacc caaatgctgc gcgacgtggc ctggggtgat cttgacgtcc
    10621 tggtgatcga catgccaccg ggcacgggtg acgtgcagct caccatggcg caacaggttc
    10681 ccctctcggg cgccatcatc gtctccacgc cgcaggatct ggccctgatc gatgcccgca
    10741 agggcctcaa catgttccgc aaggtcgacg tgccggtgct cggcattatc gagaacatga
    10801 gtaccttcgt gtgcccgcgt tgcggcgagc gcagcgacat cttcgggcac ggcggggccg
    10861 agcgcgaagc cgacaggctt ggcattgcct ttttgggcgg cgtgcccctg cacatggaga
    10921 tccgcgagaa atccgatgcg ggccaaccga tcgtggcctc caatcccgac agtgagcacg
    10981 cccggatcta ccgcgacatt gccggcaagg cctgggggca gctcgagatg agcctcgcgc
    11041 gtcggctgga gccgcccaag ctctcaatca gccccgaccg cggcgctctc gaggtggcct
    11101 tcgacggcgc ggcgccagtc agcctcagcg ccgagatgct gcgcgtcatg agcccctcgg
    11161 ccgaggtgca aggtcactct cccgaccagc gcgtgaccgt ggcgcgcaag cgcaacgtga
    11221 aaatcaagga gttgaagccc gtcggcaact atgccgcccg catcgtcttc gatgacggcc
    11281 acgataccgg gctttacagt tgggcctatc tgcgcacgct gaccgaagag cgcgagaagc
    11341 gctgggcgga gtacctttcg gctctggccc agaagggcat gagccgggat taggcgtcgg
    11401 tgctcttggc cagggctgcc tcggccggtg cggctgttgc tacggcaacg tagcctgcgc
    11461 ttggcgtgct ctgcgcgcgc gcctcgtcaa tggccgcatc gaccacgcgc tgcgcgtagg
    11521 ccggcaacgc gaaggcgccc acgtgcacgt gcggggtcca atacttgagc ccacgcacgc
    11581 ctcgcttctt ttgccgtttg gccagctgtt tggcctcgac tttgagcacg tgcttgtcgt
    11641 cggtggcgag attgagcgcg aagggcccgc cgaaatagca aggttgggtg cacaggtagg
    11701 gtgcgacgcg cttgaacagg gaggcgaata ccgccgtcgt gcccccgagg tgggccggaa
    11761 acaggaacgg caggccattc tgcgtcacga gcacgccgcc gcgcttgagc gcgcgccggc
    11821 aatcctggaa gaattcgcgc gtatgcagca ccgcgctcgg accgatcggc tcggagctgt
    11881 cgacgatgat ggcgtcaaac cgctcgtcgg tctcggccac gtacctgcag ccgtcggcaa
    11941 tcacgacgtc ggtacgtagg tcctcgaagg cggcgccggg gatgctcgga tagtgttggc
    12001 gtgccgtgtc gatgaccgtg cgatcgatct cacacagggt ggcgcgcagc accgaggggt
    12061 gcttcagcac ctcgcgcagc acgccgccat cgccgccgcc gatgatgagc acgcgcttgg
    12121 gcctctccag cgccatcagc ggcacgtgcg ccatcatttc gtggtaaacg aactcgtcgg
    12181 aggtggtgag ctggcacacc ccatcgagca ttaaaaccgt gccccaggta ggattttcga
    12241 agatgacgag gtgctggtgc tcggtcttca cctcgtgcag gacgcgcgag gcctcgagcc
    12301 gcacgcgcca atgcgggtga aacgtctctt cgatccagcg atccatgggc ccatccttcc
    12361 cgttccgacg caggggggca tagcaaagag cgcgcgcccg tcaccagccg cgcgccccaa
    12421 ctgtgcgcaa agaccgaggc ctcgcctacg ccgccttctt cgccctggtg gaccgggccg
    12481 ccgtcttctt ggcggtggca atctcccagg tgcgctcgct tgccgaattc gcgcgcagat
    12541 gttccttcac caccgtcctg tccggcctga acgccgtctc cagcaccgcg atggtcgcat
    12601 gcggatcggc gtggccgcac atgaagacgt cgagggcggc gtaaccgacc tcgggccagc
    12661 tatggatgct gatgtggctt tctgccagca ccgccacccc ggagacgccg ccattgggcg
    12721 tgaagtgatg caggtgaatg tgcaggagcg tcgcgcctgc cacctccaca caccgcttca
    12781 gcgtttcctt gatgtgtttg agatcatcaa gccgcttggc gccaaacaag tctatgagca
    12841 gatgcgttcc ggcgaaccgt acgccatcac gctcaatgaa gtagtctttc ctgtcgtcgt
    12901 gcgcgcctag cgcggtttga ccatgatctt ccttttgggc ggtgcttgaa cgggtcaagt
    12961 ccatc
//
LOCUS       DASTMT010000040         2658 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_675243, whole genome shotgun sequence.
ACCESSION   DASTMT010000040 DASTMT010000000
VERSION     DASTMT010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2658)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2658)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2658
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_675243"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..319)
                     /locus_tag="VFR00_00915"
     CDS             complement(<1..319)
                     /locus_tag="VFR00_00915"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU0157836.1"
                     /translation="MQLSMKGRNALITGGSLGIGRAIAKHFTGAGANVVLVARRQNIL
                     DEARAEVAAASNAKVVTVAADVGTAEGCRAAFAAAEAGLGHIDVLVNNAGTSQRGAFE
                     SLSD"
     gene            complement(436..1425)
                     /locus_tag="VFR00_00920"
     CDS             complement(436..1425)
                     /locus_tag="VFR00_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008129658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HEU0157837.1"
                     /translation="MASNKKKVLLTESMSRSGRNLLTERGDIEIVGFANTISEPAFQA
                     LLREAAPVNGVALGGTKFGQKEIEAAAEMLVVARIGVGFDAVDVPALTAKKIPLMVAG
                     TANSPSVAEQAMFMMMALAKRGAELNAMVQGGAWNNRLQAVPFDLFGKTVLIVGFGRI
                     GTRSAKRCQAMEMNVLVYDPYRPAAEIEAAGCEPVAELDTALPRADFVTIHCPKTPET
                     VGMFNAPRLAAMKPTAYLVNTARGGIIDEAALHKALTTGKLAGAGLDVFEQEPTPIGN
                     PLLKLANVISAPHMAGVTRESLDRMGLQTARNILSALDGSPIRENVINKEVLD"
     gene            1620..>2658
                     /locus_tag="VFR00_00925"
     CDS             1620..>2658
                     /locus_tag="VFR00_00925"
                     /inference="COORDINATES: protein motif:HMM:NF014795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEU0157838.1"
                     /translation="MATTTVEVLAEAFKDAGTPFIVGHPGGESVELMEAGRQRQMRFL
                     LMKQEVAGAMLAATWGEITGSPGVCLSTRGPGAANMVNGVAHALLDRAPLIAITDAYS
                     RPTFETGLRQRLDQKALYAPLVKWSTTIDARSVRQQLRRAIRTATGAPPGPVHFELPQ
                     SETTREAGALLAEPPLRPNLVCPRPERSDLKGVLELIGRAKRPILLAGLGVFWAKAAA
                     AFVRFAERLGAPVLTTSKCKGAIPEDHPLRAGCIIGGLIERNLITQADLIITVGLDAV
                     ELQPKPWPYTLPVLALSGAASLDGLVPADPEVVAELGGLLSALAEWAPERASWGETAA
                     RDFRAQVNQALD"
BASE COUNT          416 a          907 c          861 g          474 t
ORIGIN      
        1 catcggagag gctctcgaag gccccgcgct gcgaggtgcc ggcgttgttg acgaggacat
       61 cgatgtggcc gaggcccgcc tcggcggcgg caaaggccgc ccgacacccc tcggccgtgc
      121 cgacgtcggc cgccacggtg accaccttgg cgttgctcgc cgccgcgacc tcggcgcgcg
      181 cctcatcgag aatgttttgg cgccgggcca ccaggacgac gttggccccc gcgccggtga
      241 aatgtttggc gatggcacgg ccaatgccga gactgccacc ggtgatcagg gcattgcggc
      301 ccttcatgct cagctgcatg gaacgtctcc tcctgctctt ggctcctgct tgcttgggcg
      361 gccgcttgct tgggtcgtgg ctggttggtg ccgttgtctg gggcaccccg gcgagctagc
      421 gcgcaccgcg cccgatcaat ccaacacctc tttgttgatg acgttttcgc gaatcgggct
      481 gccgtccagc gcgctcagaa tattgcgcgc cgtctgcagg cccatacgat cgagcgattc
      541 gcgcgtgaca ccggccatgt gcggggcgct gatgacattg gcgagcttga gcaggggatt
      601 gccgatcggc gtcggctcct gctcgaaaac gtccaaaccc gcccccgcca gcttgccggt
      661 ggttagcgcc ttgtgcaggg ctgcttcatc gatgatgccg ccacgcgcgg tattgacgag
      721 ataggccgtc ggcttcatgg cggccagtcg cggggcattg aacatgccga ccgtttcggg
      781 cgtcttgggg caatggatgg tgacgaaatc ggcgcgcggc agagcggtgt cgagctcggc
      841 caccggctcg cagcccgccg cctcgatttc ggcggccggc cgataaggat cgtagaccaa
      901 cacgttcatc tccatggcct ggcagcgctt ggcgctgcgc gtgccgatgc ggccaaagcc
      961 gacgatcagc acggtcttgc caaaaaggtc gaagggcacc gcctgcagcc gattgttcca
     1021 ggcgccgccc tgcaccatgg cattgagctc ggcgccgcgt ttggcaagcg ccatcatcat
     1081 gaacatggct tgctcggcga ccgagggtga gttggccgtc cctgccacca tcagcggaat
     1141 tttcttggcc gtcagcgcgg ggacatcgac cgcatcgaag ccgacgccaa ttcgcgccac
     1201 caccagcatc tcggcggccg cctcgatctc cttttgccca aacttggttc cgcccagggc
     1261 gaccccattg acgggcgccg cttcgcgcaa aagcgcctgg aacgctggct ccgatatcgt
     1321 gttcgcaaac ccgacgatct cgatatcgcc ccgctcggtc agcaaattgc gcccgcttct
     1381 cgacattgat tccgtcagca gcaccttttt cttgttgctc gccatttccg tcccctgaac
     1441 tgagctttga cccggcagga ttacacgggt gggcggcatt tgcccacgtc ctttgcgcat
     1501 tgctgctgcc ccgcgcgttt tctaaaggcg ggccagggcg ccttggcaag ggggtacatg
     1561 cgcgggccgg cgaggttggt ataaagtcgc agcaattgat cgctttgccg gaggaacgcg
     1621 tggctacaac aaccgtcgag gtgctcgccg aagccttcaa ggacgcgggc acgccgttca
     1681 tcgtcgggca ccccggcggc gagtcggtcg agctgatgga agccgggcgc cagcggcaga
     1741 tgcgcttcct gctcatgaag caagaggtgg caggcgccat gctggccgca acctggggcg
     1801 aaatcaccgg cagcccaggg gtgtgcctgt cgacccgcgg gcccggcgcc gccaacatgg
     1861 tcaacggcgt ggcgcacgcg ctgctcgatc gggcgccgtt gatcgccatc acggatgcct
     1921 actcgcgccc gaccttcgaa accggcttgc gccagcgcct cgaccagaag gcgctctatg
     1981 caccgttggt caaatggagc accacgatcg atgcccgtag cgtgcgtcag caattgcggc
     2041 gagccatacg cacggccacc ggcgcaccgc cggggccggt gcacttcgag ctgccgcaaa
     2101 gcgagaccac gcgggaggcg ggcgccttgc tggccgagcc gccactgcgc cccaatcttg
     2161 tctgtccgcg gcccgaacgc agcgacctca aaggcgtgct cgagctcatt ggccgggcca
     2221 agcgaccaat cctgctcgcg ggtctcggcg tgttctgggc gaaggcggcc gctgccttcg
     2281 tgcgctttgc tgaacgtctc ggtgcccccg tgctgacgac ctcgaagtgc aagggcgcca
     2341 ttccggaaga ccaccccctg cgtgccggct gcatcatcgg cggcctgatc gagcgcaacc
     2401 tcatcactca ggcggacctc atcatcaccg tcgggctcga cgccgtcgag ctccagccca
     2461 agccgtggcc ctacaccctc ccggtgctgg cgctctcggg ggctgccagc ctcgatggct
     2521 tggtgcccgc cgatccggag gtcgtggccg aactcggcgg cctgctatcg gctctggccg
     2581 agtgggcgcc cgagcgcgcc agctggggtg aaaccgcggc ccgcgatttt cgcgctcagg
     2641 tgaaccaagc cctcgata
//
LOCUS       DASTMT010000041         2852 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_5588790, whole genome shotgun sequence.
ACCESSION   DASTMT010000041 DASTMT010000000
VERSION     DASTMT010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2852)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2852)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2852
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5588790"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..354
                     /locus_tag="VFR00_00930"
     CDS             <1..354
                     /locus_tag="VFR00_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEU0157839.1"
                     /translation="VTFMHDRRKSQPRQILVSLVCLCALAYFAHHAIKGKRGLEARSR
                     LIERSRVLEPQIARLEAARIRLERDVRLLNARDPDLIEELAIEVLGFARPGDRIIVPA
                     AARAMPPPASLPTRR"
     gene            438..1436
                     /gene="pdhA"
                     /locus_tag="VFR00_00935"
     CDS             438..1436
                     /gene="pdhA"
                     /locus_tag="VFR00_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009799813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HEU0157840.1"
                     /translation="MSRPNGQETVPPFNREQELTAYREMLLIRRFEEKAAQLYGMGFI
                     GGFCHLYIGQEAVVVGMKMASADGDQMITTYRDHGHMLACGMDPKGVMAELTGRRHGY
                     SKGKGGSMHMFSKEKHFYGGHGIVGANVPLGAGIAFANRYRENGSVCHTYFGDGAANQ
                     GQVYESFNMAKLWKLPVVFVIENNKYAMGTSVERSAATTDFSQRGRSFDIPGEQVDGM
                     DVRAVKATADRVLKWVRDGNGPYILEMLTYRYRGHSMSDPAKYRTREEVQKMREDHDP
                     IEQVRRRLLEGRLVSEEEMKGIDGEVRAAVNTAAEFAQSDAEPDEGELYTDVLLPA"
     gene            1465..1905
                     /locus_tag="VFR00_00940"
     CDS             1465..1905
                     /locus_tag="VFR00_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006933610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEU0157841.1"
                     /translation="MLPTAASNLDQEFERLAAFWSPRVVAMANGQYVKVAKVKGEFVW
                     HRHAEEDEFFLVRKGTLQIRYRDRPDAVLAEGDFHVVPRGVEHMTAAPEECWVVLVEP
                     AATKHTGDTQSELTTSIEQQLAHLRAPSQDTRQQETEAPWPSKS"
     gene            1887..>2852
                     /locus_tag="VFR00_00945"
     CDS             1887..>2852
                     /locus_tag="VFR00_00945"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002964257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex E1 component
                     subunit beta"
                     /protein_id="HEU0157842.1"
                     /translation="MAIEILMPALSPTMEEGKLAKWLVSEGQQVKPGDIIAEIETDKA
                     TMEVEAVDEGKVSKLLVPEGTEGVKVNTPIAVLDGEGGSIAKPAPAPSAAPPPPQPAS
                     AKRPEAPPAKAPAAPQPSERPNEPQLPAGTETANQTMREALRDAMAEEMRRDAAVFLM
                     GEEVAQYQGAYKISQGLLDEFGPKRVIDTPITEQGFAGIGVGAAMAGLKPIVEFMTWN
                     FAMQAIDQIINSAAKTLYMSGGQMGCSIVFRGPNGAAARVAAQHSQCYSAWYAHVPGL
                     KVVAPSNPADAKGLLKSAIRDPNPVLVLENEILYGASGPVPKTEDW"
BASE COUNT          539 a          954 c          897 g          462 t
ORIGIN      
        1 gtcaccttca tgcatgatcg gcgcaaaagc caaccccggc agatcctggt gtcgcttgtc
       61 tgcctttgcg cactggccta cttcgcccat catgccatta aaggtaaacg cggcctcgaa
      121 gcgcgtagcc ggctgatcga acgctcccga gtgctggagc cgcagatcgc gcgcctcgag
      181 gccgcgcgca ttcgcctcga gcgggatgtg cggctgctca acgcccgcga tcccgatctt
      241 attgaggaac tcgccatcga ggtcttgggc tttgcccgac ccggtgaccg catcatcgta
      301 cccgctgctg cccgcgccat gccgcccccg gcaagcctgc ccaccaggcg ctgaaagccc
      361 gcggccatga ctggccttca cggaccgttt cttgtacggt ttccgctccc cttcgccagc
      421 gaggcacctt atcccgcatg tcccgtccca acggccaaga aaccgttccc cccttcaacc
      481 gggaacagga actcactgcc taccgcgaga tgctgctgat ccgccgcttt gaggagaagg
      541 cggcccagct ctacggcatg ggcttcatcg gcggcttctg ccacctctat atcggtcaag
      601 aggcggtggt ggtcggcatg aagatggcct ccgccgacgg cgatcagatg atcaccacct
      661 accgcgacca cggccacatg ctggcctgcg gcatggaccc caagggggtg atggcggagc
      721 tgaccgggcg tcggcacggc tactccaagg gcaagggcgg ctccatgcac atgttctcca
      781 aggagaagca tttctacggc gggcacggca tcgtcggcgc caacgtgccg ctcggtgcgg
      841 gcatcgcctt cgccaatcgc tatcgcgaaa acggcagtgt ctgtcatacc tacttcggcg
      901 atggggccgc caaccagggc caggtctatg agagcttcaa catggccaag ctgtggaagc
      961 tgcccgtcgt cttcgtgatc gaaaacaaca agtacgccat gggcacctcg gtcgagcgtt
     1021 cggccgccac gacggatttt tcccagcgcg gtcgctcatt cgacattccc ggcgagcagg
     1081 tcgacggcat ggacgtgcgc gcggtgaagg ccaccgccga tcgcgtgctg aaatgggtgc
     1141 gcgacggcaa cggcccctac atcctggaga tgctgaccta tcgctatcgc ggccactcga
     1201 tgtccgatcc cgccaaatac cgcacgcgcg aggaggtgca gaaaatgcgc gaggatcacg
     1261 atcccatcga gcaggtacgc cggcgcttgc tggagggacg tctcgtttcc gaagaggaga
     1321 tgaaggggat cgatggcgag gtgcgcgcgg ccgtcaatac cgccgccgag ttcgcgcaat
     1381 cggatgcgga gccggacgag ggcgagcttt ataccgacgt gttgctgccg gcctgaggcg
     1441 acttcaagca aagcccggtg catcatgctc cccaccgcag ccagcaattt ggatcaggag
     1501 ttcgagcggc tcgcggcgtt ttggtcgccg cgcgtcgttg ccatggccaa cggccagtat
     1561 gtcaaggttg ccaaagtcaa aggtgagttc gtctggcatc gccatgccga ggaggacgag
     1621 ttctttctgg tgcgcaaggg tacgctccag atccgctacc gtgaccgacc cgacgccgtc
     1681 ctcgccgagg gcgacttcca tgtggtgccg cgcggcgtcg agcacatgac agctgcgccc
     1741 gaggaatgct gggtcgtcct ggtcgagccc gccgccacca aacataccgg cgacacccaa
     1801 tccgagctca cgaccagcat cgagcagcag ctggcgcact tgcgcgcccc atcccaagac
     1861 acacgccagc aagagaccga agcaccatgg ccatcgaaat cctgatgccc gccctgtcgc
     1921 ccaccatgga ggagggcaag ctcgccaaat ggctggtgag cgagggccag caggtcaagc
     1981 cgggcgacat catcgccgag atcgagaccg acaaggccac catggaggtg gaggcggtcg
     2041 acgagggcaa ggtctccaag ctgctggtgc cggaggggac cgagggcgtg aaggtcaata
     2101 cccccatcgc cgtgctcgac ggcgagggtg gcagcatcgc caagcccgcg cccgcgccct
     2161 ctgccgcgcc gccgccgccg caaccggcga gcgccaagcg ccccgaggcg ccgccggcga
     2221 aggctcccgc ggcgccccag cccagcgagc gtccgaacga gccgcaacta ccggccggca
     2281 ccgaaacggc caaccagacc atgcgcgagg ccctgcgcga tgccatggcc gaggagatgc
     2341 gccgcgatgc ggcggtgttt ttgatgggcg aggaagtggc ccaataccag ggcgcctaca
     2401 aaatcagcca gggcttgctc gacgagtttg gtcccaagcg ggtgatcgac acgccgatca
     2461 ccgaacaggg ctttgccggt attggcgtcg gcgcggccat ggccgggctc aagcccatcg
     2521 tcgagttcat gacttggaat ttcgccatgc aggcgatcga tcagatcatc aactcggccg
     2581 ccaagacgct ctatatgtcg ggcgggcaaa tggggtgttc catcgtcttt cgaggtccca
     2641 acggggcggc tgcccgggtc gccgcgcagc acagccagtg ctattcggcc tggtatgcgc
     2701 acgtgcccgg cctcaaggtg gtggcgccct ccaatcccgc cgacgccaag ggcctgttga
     2761 aatcggccat tcgcgatccc aacccagtgc tcgtgctcga gaacgaaatt ctctatggcg
     2821 cctccgggcc agtgcccaaa accgaggact gg
//
LOCUS       DASTMT010000042         2272 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_3300286, whole genome shotgun sequence.
ACCESSION   DASTMT010000042 DASTMT010000000
VERSION     DASTMT010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2272
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3300286"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            161..1123
                     /locus_tag="VFR00_00950"
     CDS             161..1123
                     /locus_tag="VFR00_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HEU0157843.1"
                     /translation="MRARLQQELGGDGSPPLAGDLDQTLAAWFAHLAGERQSSPHTLA
                     AYERDLRQFLDWLQQDQARPPSLADLTNLDAKRLRAFMAARRRAGLSSRSLARTMSAL
                     RAYFRWLEAEDVGHNRAILQIALPKVAHGIPKPLTVAKAAAVVDGAAMGELRWLAARD
                     TAVLLLLYCCGLRISEALGLRRGEAPLPGRDVIRITGKGGKERMVPVLPIAQHAVRDY
                     LDVCPYPLAAEAPLFLGAKGGPLRARIVQLLMQKLRSGLGLPQTATPHALRHSFATHL
                     LSAGADLRQIQELLGHASLSTTQVYTEVDRDHLMAVYAKAHPRA"
     gene            1218..1913
                     /locus_tag="VFR00_00955"
     CDS             1218..1913
                     /locus_tag="VFR00_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007821197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU0157844.1"
                     /translation="MAKTALIVGAGSGISAAFARALNADDYRLCLAARDIDKLAALAQ
                     QTSARLVQVDAADAGSVAALFAELDRHFARLDVVLYNASYRARGALLDLDPGEVKKSI
                     EVSAYGGFLVAQQAARRMLAEGEGAIFFTGASASVKGYAQSAPFAMGKFALRGLAQAM
                     ARELQPLGIHVAHFVIDGGVRNAARGRPDDPARPDGLLDPEAVAHTYMAVLKQPRNAW
                     SLEVELRPWLERF"
     gene            2026..>2272
                     /locus_tag="VFR00_00960"
     CDS             2026..>2272
                     /locus_tag="VFR00_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011156237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEU0157845.1"
                     /translation="MARLIELTLNGRRRVDAVPDNLLLLDYLREQAGLTGTKTGCDGG
                     ECGACTVLVDDLPRLACLTLAATVSGRRIDSIEALGEA"
BASE COUNT          335 a          783 c          756 g          398 t
ORIGIN      
        1 gtgccccggg gttgcccaag cactgccggc agctgcgcgg ccaattggtt gtaggtcgcc
       61 acatgccggc tcggcccgcg tcgggcccca cgggggctta accggtcgtt aaccgttgcc
      121 gatcgatgtt gcgctcggtt cgaacgcgga tttgagcggc atgcgcgcgc ggctgcaaca
      181 ggagctcggt ggcgacggct caccgccgct ggccggcgat cttgaccaga cgctcgccgc
      241 ctggttcgcg cacctggccg gcgagcgcca gagctctccg cacaccctgg cagcttacga
      301 acgcgacctg cggcagtttc tcgattggct gcagcaggat caggcgcgcc cgccctcgct
      361 ggccgacttg acaaatctcg acgccaagcg cttgcgggct tttatggcgg cgcggcgccg
      421 ggccgggttg tcgagccggt cgctggcccg caccatgtcg gccctgcgcg cctatttccg
      481 ctggctggag gcggaggacg tcggccacaa ccgggccatc ctgcagattg cgctgcccaa
      541 agttgcacat ggcatcccaa agcctttgac cgttgccaag gccgctgccg tggtcgacgg
      601 cgcagccatg ggcgagctcc gttggctggc ggcgcgggat acggccgtgc tgttgttgct
      661 ttattgctgc ggcttgcgca tttccgaagc gctcgggctg cggcgcgggg aggcgccgct
      721 gccgggccgc gacgtcatcc gcattaccgg caagggcggc aaggaacgga tggtcccggt
      781 actccccatc gcccaacacg ccgtgcgcga ctatctggac gtgtgtcctt atccgctggc
      841 cgcggaggcg ccattatttc tcggtgccaa aggcggcccg ctgcgagccc gcatcgtcca
      901 gctgctgatg cagaagttgc gcagcggtct tggtttgccg cagacggcaa cgccgcacgc
      961 gctgcgccat tccttcgcaa cccacctgtt gtcggcgggc gccgatctgc gccaaatcca
     1021 agagctgctc ggccacgcca gtctctcgac cacgcaggtc tataccgagg tcgatcgcga
     1081 ccacctgatg gcggtgtacg ccaaagccca tccgcgcgcc taggcgctgt caacgccgcc
     1141 ttgcggccga tgcggctcga gtgctccggt catatcggct agcatgtcgc tgccgtcaac
     1201 ctcgcggagg acgaaaaatg gccaagaccg ctctcatcgt cggcgccggc tcgggcatca
     1261 gcgccgcatt tgcccgggca ctgaatgccg acgactaccg cctatgcctc gccgcgcgag
     1321 atatcgacaa gctcgccgcg ctggcgcagc agacttcggc ccgcctcgtt caggtcgatg
     1381 ccgccgatgc cggctccgtt gcagcactgt tcgccgagct cgatcgccac tttgcacggc
     1441 tcgacgtggt gctctacaac gccagctatc gcgcgcgggg cgcgctgttg gatctcgacc
     1501 ccggcgaggt gaaaaagtcg atcgaggttt ccgcctacgg cggtttcctg gtggcccagc
     1561 aggcggcccg gcgcatgctg gccgagggcg agggtgcgat tttcttcacc ggcgcttcgg
     1621 cgagcgtcaa gggctacgcc cagtccgcgc ccttcgcgat gggcaagttt gccctgcggg
     1681 gactggctca ggccatggcc cgtgagctgc agcccttggg catccatgtc gcccacttcg
     1741 tgatcgacgg cggcgttcgc aacgccgcgc gcggtcgccc cgacgacccc gcccgccccg
     1801 acggcctgct cgaccccgag gccgtcgccc acacctacat ggccgtgctc aagcaaccgc
     1861 gtaacgcctg gagcttggag gtcgaactgc gcccctggct ggagcgcttc taggccgcaa
     1921 ttggtttggt acccaatcga tttgatggta agcttccggc tcggcgccgc cggcgcgctg
     1981 ccgcccacgg ggaaggcggc gtgctggtag ggccaggaga aggccatggc acgactgatc
     2041 gagctcaccc tcaacggccg tcgacgcgtc gatgccgtgc cggacaatct gctgctgctc
     2101 gattaccttc gcgaacaggc gggcctgacc ggaaccaaga ccggctgcga cggcggcgaa
     2161 tgtggagcct gtaccgtgct ggtcgacgat ttgccgcggc ttgcctgcct gaccttggcg
     2221 gcaacggttt ccggtcgacg gattgacagc atcgaagcgc tgggagaggc gg
//
LOCUS       DASTMT010000043         2745 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_2317205, whole genome shotgun sequence.
ACCESSION   DASTMT010000043 DASTMT010000000
VERSION     DASTMT010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2745)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2745)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2745
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2317205"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..696
                     /locus_tag="VFR00_00965"
     CDS             <1..696
                     /locus_tag="VFR00_00965"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013167169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEU0157846.1"
                     /translation="RQEPAYDSTMHFGSRLVFDGNGNLFVTLGERYYARHHAQNPSDD
                     LGKLIHITADGGIPPDNPKPDGWRPEIWSIGHRNVQGAALHPVTGRLWTIEHGARGGD
                     EINIPEAGKNYGWPVISYGRNYDGSKIGVGTHKAGMEQPVYYWDPSIAPSGATFYTGT
                     LVPEWQGNLFVGALAGQALHRMLLDGDRVVGEEILLSDLGARIRDVRQGADGALWLLT
                     DEDNGRVLRVLPR"
     gene            complement(711..1127)
                     /locus_tag="VFR00_00970"
     CDS             complement(711..1127)
                     /locus_tag="VFR00_00970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0157847.1"
                     /translation="MASRIVRLAPLRGPTAPEAASRQSLAATPRVQVWGGASGQRYAH
                     AVYSLLECPPLPQAVYLLVQRRQDGTLKVLYIASALSAAPTLNLARIRQRGATLGANE
                     VHAYLPSSGADACRTIACDLRAGLFGSLGAEPTPGS"
     gene            complement(1291..>2745)
                     /locus_tag="VFR00_00975"
     CDS             complement(1291..>2745)
                     /locus_tag="VFR00_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013949670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase,
                     adenosylcobalamin-dependent"
                     /protein_id="HEU0157848.1"
                     /translation="SVRFLDDIVDVSNYPLLPQKKEALGKRRIGLGVTGLADALIMCG
                     ARYGTPAAVALAEQWMGALNVAAYAATAELAREKGPFPLYNAERFLTAPNLAKLPESV
                     RAQIAQFGARNGLLTSIAPTGTISLLAGNVSSGIEPIFDFRHERRVLQRDGSARSEVV
                     EDYAHALFRARFGANAPLSEAFVTAQELTPRAHLEMQAALQRHVDSSISKTINCPADI
                     GFEAFKDVYLDAYDLGLKGCTTYRPNAVTGAVLSRAAAAAEPAAPPEATPATAPQPGP
                     ETRTPTPTGRSVERAGEIVYMAQPLQREPVLEGLTYKLRWPSSDHAIYITINDINQAG
                     RRRPFEIFINSKNLEHYAWTVALTRMISAIFRRGGDVTFVVEELKAVFDPQGGQWING
                     RYVPSLLAAIGGIVENHMLRIGFLAPRADPEDTTLAQPFALAASQERAAGGPPEGSTT
                     PLARGRICPRCNHPALTHREGCWICDNCSYSKCG"
BASE COUNT          468 a          857 c          933 g          487 t
ORIGIN      
        1 cgtcaggagc ccgcgtacga ttccaccatg catttcgggt cgcgcctggt gttcgacgga
       61 aacggcaatc tgtttgtgac gctcggggag cgctactacg cccgtcatca cgcgcagaat
      121 ccgtctgacg atctgggcaa gctcatccac atcacggccg acggcggcat tccgccggac
      181 aatccgaagc cggatggctg gcgacccgaa atctggtcca tcggtcatcg caacgtgcag
      241 ggcgccgcgc tgcatcctgt cacgggaagg ctatggacga tcgagcacgg cgcgcgtggc
      301 ggcgatgaaa tcaacattcc cgaagccggt aaaaactatg gctggcccgt catcagctac
      361 ggccgcaact acgatggcag caagatcggc gtgggcacgc acaaggccgg catggagcag
      421 ccggtctatt attgggaccc ctccatcgcc ccctcaggcg ccacattcta taccggtact
      481 ctcgttcccg aatggcaagg caacctgttt gtcggcgccc tcgccgggca ggcgctgcac
      541 cgcatgctac tcgacggcga tcgggtggtt ggtgaggaga tcctgctgtc cgacctgggc
      601 gcgcgcattc gcgacgtccg gcaaggggcc gatggtgccc tttggctctt gaccgatgaa
      661 gacaacggac gcgtcttgcg cgtcctgccg cgctgaggcc gacggttcgg ctagctgccc
      721 ggagtcggtt ccgcgcccag gctcccgaat agccccgcgc gcaggtcgca ggcaatcgtt
      781 cgacaggcgt ctgcgccact gctcggcaag taggcgtgga cttcgttggc gcctagcgtg
      841 gcaccgcgct ggcggatgcg ggcgaggttc agcgtgggcg ccgcgctgag cgcagaggcg
      901 atatagagca ctttcagcgt gccatcctgc cggcgctgca cgaggaggta gacggcctgc
      961 ggcagcggcg ggcactcgag caaactgtag acggcgtgcg catagcgctg accggaggcg
     1021 ccaccccaga cctgcacccg gggtgttgct gccagggact ggcgcgaggc cgcctcaggg
     1081 gcggtgggcc ctcgaagcgg ggcgagcctg acgatacgcg aagccatgtc gagaagtttg
     1141 ccgctgggtg gctatcgatg cttgcagtgc tggtagctcc gattcgcagc cgaaatatgg
     1201 cttcgaccgt gttatttcaa ggcattgccg ccttcggcgg tgatcccttc tcggcccgaa
     1261 cctgttaacc acgcttgccg gcggtcggtt tcacccacat ttcgaatagc tgcagttgtc
     1321 gcagatccag cagccttcgc ggtgcgtgag ggccgggtga ttgcagcgcg gacagatccg
     1381 gccgcgcgcc agcggagtgg tgctgccctc tggcgggccg ccagcggccc gctcctggct
     1441 cgccgccaaa gcaaagggtt gggccagggt ggtgtcctct gggtcggcgc gcggcgccaa
     1501 aaagccgatc cgcagcatat ggttctcgac aatcccacca atggctgcca gcaggctcgg
     1561 cacatagcgc ccgttgatcc attggccgcc ctgtgggtcg aaaacggctt tcagctcctc
     1621 cacgacgaag gtgacgtcgc cgccgcggcg gaaaatggcg ctgatcatgc gcgtcagggc
     1681 cacggtccag gcgtagtgtt ccaagttctt ggaattgatg aagatttcga aaggacgccg
     1741 gcggcccgct tggttgatgt cgttaattgt aatgtagatc gcatggtcac tgctgggcca
     1801 tcgcagcttg taggttagcc cttccaacac gggctcgcgc tgcagcggct gggccatgta
     1861 gacgatctcg ccggcccgct ccacgctgcg ccccgttggg gttggcgtgc gcgtttcggg
     1921 cccgggctga ggcgcggtgg cgggcgtcgc ttcgggcggc gctgcgggtt cagcggccgc
     1981 ggccgcgcgg ctcagcaccg cgccggtgac ggcgtttggc cggtaggtcg tacaaccctt
     2041 gaggcccaag tcgtaggcgt cgagatagac gtccttgaag gcctcaaagc cgatatccgc
     2101 cggacagttg atggtcttgg aaatcgagct atcgacgtgg cgctggagag cggcctgcat
     2161 ttcgaggtgg gcgcgcggcg tcagctcctg cgccgttacg aaagcctcgc tcaggggagc
     2221 attggcgccg aagcgggcac gaaatagcgc atgcgcatag tcctcaacga cctcgctgcg
     2281 cgcggagccg tcgcgctgca acacgcgacg ctcgtggcga aaatcgaaga tgggctcaat
     2341 cccgctcgaa acgttgccgg ccaggagcga gatcgtcccc gttggggcga tcgaggtgag
     2401 aaggccgttg cgcgcgccaa attgtgcgat ctgggcgcgc acgctttcgg gaagcttcgc
     2461 caggttcgga gcggtcagga aacgctcggc attgtagagc ggaaaaggcc ccttctcccg
     2521 cgccagctcg gccgtcgcgg cataggcggc gacgttgagc gcccccatcc attgctcggc
     2581 gagcgcgacg gcagccggcg taccatagcg tgcgccgcac atgatcagcg cgtcggcgag
     2641 cccggtgacg ccgaggccaa tgcggcgctt gccaagcgcc tctttttttt gcggcagaag
     2701 cggatagttg gagacatcga cgatgtcatc caaaaagcgc acgct
//
LOCUS       DASTMT010000044         2255 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_1332919, whole genome shotgun sequence.
ACCESSION   DASTMT010000044 DASTMT010000000
VERSION     DASTMT010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2255
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1332919"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            294..524
                     /locus_tag="VFR00_00980"
     CDS             294..524
                     /locus_tag="VFR00_00980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0157849.1"
                     /translation="MSTLTYTNSDYDPRAAASASPHKPVWRRAYDAVMQSQQRRAERE
                     IAAYLSRHGGLLTDDMEREIMLHLEGRRRGTL"
     gene            842..1120
                     /gene="infA"
                     /locus_tag="VFR00_00985"
     CDS             842..1120
                     /gene="infA"
                     /locus_tag="VFR00_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011474121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEU0157850.1"
                     /translation="MAKEEMLEFEGVVAEVLPDARCRVKLDNGHEVVAYTSGRMKKNR
                     IRILAGDRVTVEMTPYDLDKGRINFRHKDVRAPVNTGPPRRPPQRRFR"
     gene            1316..>2255
                     /locus_tag="VFR00_00990"
     CDS             1316..>2255
                     /locus_tag="VFR00_00990"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEU0157851.1"
                     /translation="MSERASSRRKTREVSPLGAVAGRAPAPKFDKKTIALVYDFDGTL
                     SPRPMQEYAFLPKIGADPAAFWAETNALARQARADPLITYMHLMYKKAKDKGVRIDRR
                     DLVDQGRQVELFAGVETWFDSIGGYVKSHAQSHGVQLRHYLISSGLSEIVEGTSIYKR
                     FHNVFASEYWFEAYDLPYPKRVITDTGKTQYLFRINKGVEDLGQSINQHMAEDERPIP
                     FANMIYFGDGDTDVPSMAVMRKNGGHAVAVHPPGKGKAKCVELFKAGRCDFFAPADYR
                     RGSDLFKRTCLLIDRIIADIRVQEEVWRVGRSAARSA"
BASE COUNT          428 a          731 c          738 g          358 t
ORIGIN      
        1 gcctaagccg gcgcggcatc ggggtgcttt tgaagggtgc ttcgaccccc acgcgcccca
       61 ggcgtgcgcc gcggctgcgg cgcgctgaag cgcgagcgcg acctatgcgg acggcgcatt
      121 gctgcagcgc agcaaagacc cctactttag ttgcaagcaa ggcccaaatc agcaactgcg
      181 gacggccgcc ggcagtcgag ccggcgcgcc gcgaaggccc cgaccgacaa ggggccccga
      241 cacggctctg gggggaaggc acgaggttgg cacccagaaa gaggagctga tccatgagca
      301 ccctgactta cacaaacagc gattacgacc cgcgcgcggc cgcatccgcg agcccgcaca
      361 agccggtctg gcggcgcgcc tatgatgccg tcatgcaatc ccagcagcgg cgcgccgaac
      421 gcgagatcgc ggcctatctc agtcgccacg gcgggctcct gacggacgat atggagcgcg
      481 aaatcatgct gcatctggaa ggtcgccggc gcggcacgct ctagagcctt ttttgtgccg
      541 gtttcggtgc gggcgcagcc cgcgggtttg ctcgacgtca aaagttgaac gatgacaggg
      601 gcacagcgct cacattgacg tgagcgctgt gccggccgct tggcgtgccg cgttttcgct
      661 tgccggcgct gcgccggcgg cccatatacg ggcagcaaaa atcagcaggg aaccccgctt
      721 gacggcgtcg gcacgtgacc ccgcgcgatt ggcgcattga attgagccgc gctctgaccc
      781 gaagcgcgcc gcagatcggg tgtcgcaagc agcaaggtgg gccgtcgaat aaggagactg
      841 catggccaag gaagagatgc tggagttcga aggtgtggtc gcggaggttt tgccggacgc
      901 gcgctgtcgc gtgaagctgg ataatgggca tgaagtcgtc gcctatacct ccgggcgcat
      961 gaaaaagaac cgcatccgta tcctggccgg tgatcgcgtg accgtggaga tgacgcccta
     1021 cgatctcgac aagggtcgca tcaacttccg ccacaaggac gtgcgggccc cggtcaatac
     1081 cgggccgcca cgccgtcccc cccagcgccg cttccgctga caatcgatcc tcgccgctgc
     1141 gggcgcaaat tggccccgca caatcgcttt ggcggcggcc aacggcttgt ggcaggaggc
     1201 attcgcgcgc cccgtggctg ttgccggcgg gggacgacgc acaaaggccg ccttggtcgg
     1261 gccatccgaa tcggccgatg ctagggtccc gctccaggac gggagccaga aaggcatgag
     1321 cgaacgcgca agttctcggc gcaaaacccg cgaggtttcg ccgttgggcg ccgtcgctgg
     1381 ccgcgcgccg gccccgaaat tcgacaaaaa aaccatcgca ctcgtctatg atttcgacgg
     1441 cacgctgtcg ccgcggccga tgcaggagta tgcattcctg ccaaagattg gcgccgaccc
     1501 cgccgccttc tgggccgaga ccaacgcgct cgcgcggcaa gcgcgcgccg acccgctgat
     1561 cacctacatg cacctcatgt acaagaaggc gaaggacaag ggtgtgcgca ttgatcggcg
     1621 cgatctcgtc gaccagggcc ggcaggttga gctgtttgcg ggcgtcgaga cctggttcga
     1681 tagcatcggt ggctacgtca agagccatgc ccagagccat ggcgtgcagc tgcggcacta
     1741 cctgatctcc tcggggctca gcgagatcgt cgagggcacc agcatctata agcgcttcca
     1801 caacgtgttt gcctccgaat attggtttga agcctacgat ttgccctacc ccaagcgtgt
     1861 gatcaccgac accggcaaga cccagtacct gttccgcatc aacaagggcg tggaggacct
     1921 gggccagagc atcaaccagc acatggccga ggacgagcgg ccgatcccgt tcgccaacat
     1981 gatctacttc ggtgacggcg acaccgacgt gccgtcgatg gctgtgatgc gcaagaacgg
     2041 cgggcacgcg gtagccgtgc atccgcccgg caagggcaaa gccaagtgcg tggagttgtt
     2101 caaggcaggc cgctgcgact tcttcgcgcc cgccgattac cgccgtggct ccgatctctt
     2161 caagcgcacc tgcctgctga tcgaccgcat cattgccgac atccgagtgc aggaggaggt
     2221 ctggcgcgtc ggccgcagcg cagcgcggtc ggccg
//
LOCUS       DASTMT010000045         2223 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_2973494, whole genome shotgun sequence.
ACCESSION   DASTMT010000045 DASTMT010000000
VERSION     DASTMT010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2223
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2973494"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..623
                     /locus_tag="VFR00_00995"
     CDS             <1..623
                     /locus_tag="VFR00_00995"
                     /inference="COORDINATES: protein motif:HMM:NF014869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M4 family metallopeptidase"
                     /protein_id="HEU0157852.1"
                     /translation="HGIIAHEADLVYADESGAVNEALADIFGTLIEFQARDGAGNWAI
                     GESLPGYSEASPMRSMAEPHLKGQGNASLFNKAQPFSVTNRGQPDHYSEYVARSDSLC
                     DTTWDYLNGCVHFNSGVLNKLAYLVSEGGEHKGQTVSGIGRLKLARIVYRALTTKLNA
                     TSSLVHAADSFVAACTDLVGAVAGITAQDCVQVENARRAVGLGTDS"
     gene            845..1792
                     /locus_tag="VFR00_01000"
     CDS             845..1792
                     /locus_tag="VFR00_01000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0157853.1"
                     /translation="MLQPIFTDFAPQHTDLFGRHTVHLGHRFDGADSLFGDAALASLI
                     ERTPDLSTHVNTMDATSQDPRTRREGTLKGLSGAEALDAVRRGRIWILLQQPTEIDRR
                     YAEVLRAIYAELEVRVPGLRSFKHKMSILISSPKVQVYYHADVPGQTLWQVRGSKRVY
                     VYPNTPPFLPQAALEKIVLGEAHEISLQYEPWFDAHAEVIQLEPGRMLHWPLNCPHRI
                     INDDCLNVSFTTEHMTRELRNAYAVNYANGVLRRALGLERLPRPQTGLGLYARLGLAA
                     AHKYSGAQARGRAKRIIDFEVDPAAPKGVRDVAPFALRA"
     gene            1915..>2223
                     /locus_tag="VFR00_01005"
     CDS             1915..>2223
                     /locus_tag="VFR00_01005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0157854.1"
                     /translation="MRHVNVLSIQRISAEEKRQIEAVDAAVRLTDAGGWFDGEIRDTW
                     PAFTAARYLAPDAAGAGSRAERDRLLAEAEVILGGWPFPRDLRARAPKLKWFHQRPAG
                     A"
BASE COUNT          387 a          733 c          721 g          382 t
ORIGIN      
        1 cgcacggcat tatcgcgcac gaggcggatc tcgtttatgc cgacgaatcg ggtgcggtga
       61 acgaggccct cgccgacatt ttcggcacct tgatcgagtt tcaggccagg gacggcgccg
      121 gcaattgggc gatcggggag agcttgcccg gttattcgga ggcgagcccc atgcgcagca
      181 tggccgagcc gcacttaaag gggcaaggca acgcctcgtt gttcaataaa gcccagccgt
      241 tttcggtgac caatcgcggc cagccggacc actattccga gtacgttgcg cgcagcgaca
      301 gcctgtgcga cacgacctgg gactatctca atggctgcgt gcatttcaac agcggcgttc
      361 tcaacaagct tgcctatctc gtatcggagg gcggcgagca caagggccag acggtgagcg
      421 gcatcggtcg tctcaagctc gcacgcatcg tgtaccgtgc tttgacgacg aaactcaatg
      481 ccacctcgag cttggtgcat gcggcagaca gcttcgtagc cgcctgcacg gacctcgttg
      541 gcgcggtggc gggaatcacg gcccaggatt gcgtgcaggt cgagaatgcc cgtcgtgccg
      601 tgggcctcgg caccgatagc taggcagggt cctcccgggg acccgggagg ctgagccgcc
      661 gggacggcgc taaccccgca atgcacattt gagcgccgag gagcgagcgc gctccccgag
      721 ctcgcccgtg ttccaaatct ggacagcggc tcgcgccccg cgggcgtgcg cccagccccg
      781 ggttggcgcg ctttgtgcag tgacccaggc tcgcccgctc aggtgtgccg aagtggtccc
      841 gcccatgctc cagcctatct ttaccgactt tgcaccccag cataccgatc tgttcggtcg
      901 gcataccgtg cacctcggcc accgcttcga cggagcggat agcttattcg gcgatgcggc
      961 gctcgccagc ctgatcgagc gcactcccga cctctcgacc catgttaaca ccatggacgc
     1021 gacgagccag gatccgcgca cgcgccgcga aggcacgctc aaggggctct ccggggctga
     1081 ggcgctcgac gccgttcggc gaggccgtat ttggattttg ttgcagcaac ccaccgagat
     1141 cgaccggcgt tatgccgagg ttctgcgggc gatctatgcc gagctcgagg tgcgcgttcc
     1201 cggtctgcgc tccttcaagc acaaaatgtc gatcctgatt tcctcgccca aggtgcaggt
     1261 ctactatcac gccgatgtgc cgggacagac gctgtggcaa gtgcgcggca gcaagcgcgt
     1321 ctacgtctac cccaacacgc cgccgttctt gccgcaggcg gcgctggaga aaatcgtttt
     1381 gggcgaggcg cacgaaatct ccctccaata cgagccctgg ttcgatgccc acgccgaggt
     1441 cattcagctg gagcccggcc gcatgctgca ttggccgctc aactgtccgc accggatcat
     1501 caacgatgat tgcctcaacg tctccttcac gaccgaacat atgacgcgcg agctgcgcaa
     1561 tgcctatgcg gtcaactacg ccaatggtgt tctgcgccga gcgctagggc tcgagcgctt
     1621 gccccggccg cagaccggcc ttgggcttta tgcgcggctg ggtctggcgg ccgcgcataa
     1681 atattcgggc gcccaagcgc gcgggagggc aaaacggatc atcgacttcg aggtcgatcc
     1741 ggcggccccc aagggcgtgc gcgacgtggc gccctttgcc ctgcgtgcct agcgcaaccg
     1801 gcacctggac ggggcgggca caaatgctgc cgtaaacgga aaaaatcctc gacggggcgg
     1861 tggcgaggag aggatgatgc cggcggccaa gcggtccagc tgccaggagc cggcatgcgt
     1921 cacgtcaacg tcttgagcat ccagcgcatc agcgccgagg agaaacggca gatcgaagct
     1981 gtcgatgcgg ccgttcgact gaccgacgcc ggcggatggt tcgacgggga gatccgcgac
     2041 acctggcccg cctttaccgc ggcgcgttac ctggcgcccg atgccgccgg cgccggcagc
     2101 cgggccgagc gcgatcggct gcttgccgag gcggaggtca tcctgggcgg ctggcccttc
     2161 ccccgggatt tgcgtgcccg agcgccaaag ctcaagtggt ttcaccagcg gcccgccggc
     2221 gcc
//
LOCUS       DASTMT010000046         3182 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_3301432, whole genome shotgun sequence.
ACCESSION   DASTMT010000046 DASTMT010000000
VERSION     DASTMT010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3182)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3182)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3182
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3301432"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..200
                     /locus_tag="VFR00_01010"
     CDS             <1..200
                     /locus_tag="VFR00_01010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0157855.1"
                     /translation="IAERHAFPAYYAAPFLFEPDWSQGSDCYVPLSDDSAADRELGTI
                     FKTSRRVLLDEGRPLLPEYPG"
     gene            complement(503..1054)
                     /locus_tag="VFR00_01015"
     CDS             complement(503..1054)
                     /locus_tag="VFR00_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018181509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEU0157856.1"
                     /translation="MGQAGVDPTGGLEVFEIAGTIKWFDISKGYGFIVPDNGLPDVLL
                     HVTCLRAGGYQTAYEGARVHCQVLNRPRGMQAFRVLSMDESTAIHPSQLPQRTHVVVN
                     AESDWERAMVKWFNRVRGFGFLTRGEGTPDIFVHMETLRRFGFTELRPQQTVQVRWGM
                     GVKGCMAAELRPDGAPPSFPSAH"
     gene            1635..2075
                     /gene="gloA"
                     /locus_tag="VFR00_01020"
     CDS             1635..2075
                     /gene="gloA"
                     /locus_tag="VFR00_01020"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012330255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004462 - lactoylglutathione lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0051596 - methylglyoxal catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HEU0157857.1"
                     /translation="MRYLHTMIRISDIEKSLDFYCNKLGMREVRRMDSEAGRYTLIFL
                     AAPGDAGKAGAEQSPLVELTYNWDPEAYGEGRNFGHLAFEVDDIYATCDRLQKAGVTI
                     NRPPRDGRMAFIRSPDHISIELLQKGASKPQQEPWASMPNTGKW"
     gene            complement(2154..2810)
                     /locus_tag="VFR00_01025"
     CDS             complement(2154..2810)
                     /locus_tag="VFR00_01025"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009031820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEU0157858.1"
                     /translation="MPAATRWWWIRHAPVPDGGRIYGQRDLACDCSDREAFAGLAAEV
                     PAEAVWVTSHLVRTTQTARAIHAADPGKFAGVELLALPELAEQHLGDWQGLDRQAFYA
                     ARGIGTHMLWLAEADERAPGGESFADLMRRVAPAIDRLNAAHRGRDIVAVTHGGTIRA
                     ALGLALGIPPRAALAFSTENCSLTRLDHLPGAGGAGVWRVVAVNRRLARAAPAGSAAI
                     "
     gene            complement(2823..>3182)
                     /locus_tag="VFR00_01030"
     CDS             complement(2823..>3182)
                     /locus_tag="VFR00_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012110277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEU0157859.1"
                     /translation="ENGADVIYDPVGGDYAEAALRSIAWEGRFLVIGFPAGIPKLPLN
                     LTLLKGCQVVGVFWGGFSQRDPKANAANIAELMQLYAQGKIRPLVSERYPLARAGEAI
                     ARLAGRQAMGKIVVTMG"
BASE COUNT          569 a         1100 c         1011 g          502 t
ORIGIN      
        1 tgatcgccga gcggcatgcc tttccggcct actacgccgc gccttttctg ttcgagccgg
       61 actggtcgca gggcagcgac tgctatgtcc cgctgtccga tgacagcgcc gccgatcgcg
      121 aactcggcac catcttcaag acgagccggc gggtcttgct cgacgaggga cgccctctcc
      181 tcccggagta tcctggctga agcgcccacc aagctgtacg gggcaggcaa gcgatccctt
      241 gggcctcgct tgcgctcaat cacaacctgc acacgaacgc agcacggcgc accaacctcc
      301 gccatacgat cattggacat aagagcaaaa ggcgaagcct gggcattcgc ctgagagccg
      361 tgcagcgggc gccggaaatt tgaaaagggc cgctatcgag agcagccccc ctgaagagcc
      421 aaccggcccg ggtcgccggg ggagccgggc gcaccggtcg cgccagtgct cggccaaacc
      481 ctcgccgacg gcggcggtgc cgctagtgtg ccgacgggaa actcggcggc gcgccgtcgg
      541 gcctcagctc ggccgccatg cagcccttga cgcccatgcc ccagcgcacc tggaccgtct
      601 gctgcggcct cagttcggtg aagccgaacc ggcgcagcgt ctccatgtgc acgaaaatat
      661 cgggcgtgcc ttcaccgcgc gtcaggaagc cgaagccgcg cacgcggttg aaccatttga
      721 ccatggcccg ttcccagtcg ctctccgcat tcaccacgac gtgggtgcgc tgcggcagct
      781 gggaggggtg gatggcggtg ctctcgtcca tgctgagcac ccggaaggcc tgcatgcccc
      841 tgggacggtt cagcacctgg caatggacgc gggcgccctc gtaggccgtc tggtagccgc
      901 cggccctgag gcaagtcaca tgcaagagaa cgtccggcag tccgttgtcg ggaacgatga
      961 agccgtatcc cttggagatg tcgaaccact tgatggtccc ggcaatctcg aagacctcaa
     1021 gccccccggt cgggtcaacg ccggcctgac ccacgtcaat aggctcagcc ccccgctgag
     1081 ggggcaactt gtcgtcaccc ataagcttac cccgcttact gggcgccgcg accgagtttg
     1141 ccctcatcga tcacgacgca ttccgaacgg ggcaaaactg cctctcttgt gatggtaatg
     1201 tccagttttt attgccacat attatcgacg actgtgcaga accaaaactt catccatctt
     1261 atcaataggt tagaagcggc gatcattagc ttccagtgta gcacaaaagc atcacattgc
     1321 atcggaaact aagcgctgtt gataaattgg ggaactattg ggccaaaccg attcgcgctt
     1381 aacgggcgcc ttcgaatcga gaaatcggga gactcaattc acagcggcgg cgctgaaatc
     1441 gattcgcatt gctatggcaa cgcttacgcg gtccggctcg catcctgcac gaggggttat
     1501 cctcaggttg tccagcgcag acgaccggct atgatcgggc ccagccgcgc cagcgggggt
     1561 tacacacatc ggccccccct gcatccgtca tcattccaca tagttccacg aaatgggccg
     1621 aaaaggaggc aagcgtgcgc tatctccaca ccatgatccg gatctccgac atcgagaaga
     1681 gcctcgactt ctactgcaac aagctcggca tgcgcgaggt gcgccgcatg gacagcgagg
     1741 ccgggcgcta caccctgatc ttcctcgccg cgcccggcga tgccggcaag gccggcgccg
     1801 agcagtcgcc actggtagag ctgacctata actgggaccc ggaggcctac ggcgaggggc
     1861 gcaacttcgg gcacctggca ttcgaggtgg acgacatcta cgccacctgc gaccgcctgc
     1921 agaaggccgg cgtcaccatc aaccgcccgc cgcgcgacgg ccgcatggct ttcatccgct
     1981 cgcccgacca catctccatc gagctcttgc agaagggcgc ctccaagccc cagcaggagc
     2041 cgtgggcatc gatgccgaat accgggaagt ggtaaggcgc cctctcgccc tggcatctcc
     2101 ggcctcgcct cgcgcctcca cggcgcgggg aggatccagt gtgagcgcat cgctcaaatg
     2161 gcggcagagc ccgcgggggc cgctcgcgca agacggcggt tgacggcgac gacccgccat
     2221 acgccggcgc cgccggcgcc gggcagatgg tcgaggcgcg tgagcgagca gttttcggtg
     2281 gagaaggcca gcgcggcccg gggtgggatg cccagcgcca gccccagcgc ggcgcggatg
     2341 gtgccgccgt gcgtcacggc gacgatgtcg cggccgcggt gcgcggcgtt gaggcggtcg
     2401 atggcgggag cgacgcgccg catgaggtcg gcaaagcttt cgccgccggg cgcgcgctca
     2461 tcggcctccg cgagccacag catgtgcgtg ccgatcccgc gggccgcata gaacgcctgc
     2521 cggtcgagcc cctgccagtc gcccaaatgc tgctcggcaa gctccggcag ggccagcagc
     2581 tcgaccccgg cgaacttgcc cgggtcggcg gcgtggatcg cccgtgccgt ctgggtggta
     2641 cgcacgagat ggctcgtcac ccagaccgcc tcggccggca cctcggcggc aaggcctgca
     2701 aaggcttccc tgtcgctgca atcgcacgcg aggtcgcgct ggccgtagat gcgcccgccg
     2761 tccggcaccg gcgcgtgcct gatccaccac catcgcgtcg cggccggcat gagacccgcc
     2821 cctcacccca tcgtcacgac gatcttgccc atggcctgcc ggccggcaag gcgggcgatc
     2881 gcctcgcccg cccgggcaag cggatagcgc tcggagacca gcggcctgat cttcccctgg
     2941 gcgtagagct gcatcagctc ggcgatgttg gcggcgttgg cctttgggtc gcgctggctg
     3001 aagccgcccc agaacacgcc cacgacctgg cagcccttga gcagggtgag gttgagcggc
     3061 agcttcggga tgccggccgg gaagccgatc accaggaaac ggccctccca ggcgatcgag
     3121 cgcagcgccg cctcggcata gtccccaccc accgggtcgt agattacatc ggcgccgttc
     3181 tc
//
LOCUS       DASTMT010000047         1616 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_22023, whole genome shotgun sequence.
ACCESSION   DASTMT010000047 DASTMT010000000
VERSION     DASTMT010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 1616)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1616)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1616
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22023"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1085)
                     /locus_tag="VFR00_01035"
     CDS             complement(<1..1085)
                     /locus_tag="VFR00_01035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0157860.1"
                     /translation="MPQPTPIKPGPSSQADAHVWRREHAGSAPSHRGAAAEAGEAAWH
                     HNAAFEQLASRVECLSARIEATRSALGVEGVGLFEGVADALAALKTGIVSVRCAWESD
                     DPWLERLPPRQARPVSVAVPSSTAETDPWDAESAEALTRVCEIAALEDQQPRAGRRPG
                     STRATEPLPERASDLDGRLADLADRLQQALPGLDPARWLAPLDQRIRQLEQAVAATST
                     RATGAPEAESLIALEMLIRELAVQLEHTRAELGRLDAVDAHLRDMVGQLKDLAATAKP
                     STPAPPGSERDGARRVEALLQTSLAARRQDARAAVGVLKSIHAAVGQLAARLDALRPP
                     ERSPDQDLALQIDPDADRDLLLKAYREG"
     gene            1381..>1616
                     /locus_tag="VFR00_01040"
     CDS             1381..>1616
                     /locus_tag="VFR00_01040"
                     /inference="COORDINATES: protein motif:HMM:NF017215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase N-terminal
                     domain-containing protein"
                     /protein_id="HEU0157861.1"
                     /translation="MAWRIGVDIGGTFTDVALIEEASGRIGVAKALTTPADFTEGVLC
                     ALRTAMRHYAISPADVDLLAHATTVVTNAILEEKG"
BASE COUNT          243 a          541 c          572 g          260 t
ORIGIN      
        1 ccttcgcgat aggccttgag gaggagatcg cgatcagcgt cgggatcaat ttgcagtgcc
       61 aggtcctgat cgggcgagcg ctccggggga cgcagcgcgt ccaagcgcgc ggcgagctgg
      121 ccgacggcag catgaataga cttgagcacg ccaacggcgg cccgcgcatc ctgtcgccga
      181 gcggcgaggc tggtttgcag caacgcctct acgcgacggg cgccatcgcg ttccgagcct
      241 ggcggcgccg gcgtcgacgg cttggccgtc gccgccaaat ctttgagttg tccaaccatg
      301 tcacgcaggt gggcgtcgac ggcatcgaga cggccgagct cggcgcgggt gtgttcgagc
      361 tgcaccgcca gctcgcggat cagcatttcc aaggcaatga ggctttcagc ctccggcgca
      421 cccgttgccc gcgtcgaggt tgcagccacc gcctgctcga gctggcggat gcgttggtcg
      481 agcggggcca accagcgtgc ggggtcaagg cccggcaacg cctgctgcag gcggtcggcg
      541 agatcggcca atcggccgtc caggtcgctt gcgcgctctg gcagcggctc ggttgcccgc
      601 gtcgacccgg ggcggcggcc cgctcgaggt tgctggtcct ccagcgccgc gatctcgcaa
      661 acgcgcgtca aggcctcagc gctttctgca tcccacggat cggtctcggc cgtgctcgat
      721 ggcaccgcca cgctcacggg ccgcgcttgc cgtggcggca gccgttcgag ccaggggtcg
      781 tcgctttccc aggcgcagcg cacgctgacg atacccgttt ttagggccgc gagcgcgtcg
      841 gccacgcctt cgaataggcc cacgccctcg acgccgaggg ctgaacgggt cgcctcgatc
      901 cgcgcggata ggcattcgac gcggctggcc aactgttcga aggccgcgtt gtgatgccag
      961 gcggcctcac ccgcctcggc cgccgcgcca cggtgggagg gggcagagcc cgcgtgctcg
     1021 cggcgccaga catgggcgtc cgcttgcgag ctcgggccgg gcttgatggg ggttggctgt
     1081 ggcatgtgac tatcccgggg cagcaaaagg caggcgcggc gcgcctggcg accggcaggc
     1141 aatggaggcg gccttgtcgt gccggccaca ggcaaagccg gcggccgcac aaacaccaca
     1201 cgttcaaagg ctcccattca gcctcgcaat ctgccgtaaa gtttcggtta agtgcgcgta
     1261 tgccgctgcg aagaagccgt tcgtgtagcg gccgcgcgcg ccgctgcgtg tcctcgcccc
     1321 gtcgttgccc gggccggcag aacctgccta cacttcgccg ctaacagcga gggagcgggc
     1381 atggcctggc ggatcggcgt tgatattggc ggcaccttta cggacgtggc gctgatcgag
     1441 gaggcgagcg gtcgcattgg ggtagcgaag gcgctcacca caccggccga ctttaccgag
     1501 ggcgtgctct gcgcgctgcg cacggccatg cggcactatg ccatcagccc ggccgatgtc
     1561 gatctgttgg cgcacgccac cacggtcgtc actaacgcca ttttggagga aaaagg
//
LOCUS       DASTMT010000048         2294 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_4277831, whole genome shotgun sequence.
ACCESSION   DASTMT010000048 DASTMT010000000
VERSION     DASTMT010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2294)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2294)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2294
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4277831"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1949)
                     /locus_tag="VFR00_01045"
     CDS             complement(<1..1949)
                     /locus_tag="VFR00_01045"
                     /inference="COORDINATES: protein motif:HMM:NF020037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEU0157862.1"
                     /translation="MRPELSPGPTLSMALVESLRALRLLLPQNQLKDASMAEIEVGQT
                     SPADTTIARVHALGGAFSLLGMMLAVALVLVLLTNSMAEPMVVGLLALLAVAGVFLVF
                     GLLSGFLRLGDRAAEADVIRAVAEGLDSALQIVNAQGSVLYRNRALERLTGRRSGRHA
                     SLEELFAGAPDTAQAYFRLSRAAERLEGRQEEIYVHPGPLGGRSGRWLNVSVRPFPDP
                     APSKMRRRLTLWQIDDVTRERMRESETVSGLESTLAFYDGLPQGLLAVLPDGRLAHLN
                     ATLAQWLNLSPDAGRSLTLRDIVSPDGAALIQASGRTGNGRTSRFDLDLLREDGRRFP
                     AHLICRAHGSKGAVAVLVLDRSAEPAGVADSERQLVRFLHSAPFGMATVDADGAILNA
                     NPAFMRMFSLDERGLPTGIADLGGNGGEDVRRELRKAVRRAASGRAAAGPIEISFGAE
                     RELVRRVYVSALSSDARERDGAILYAVDATEQKALELKFAQSHKMEAVGQLAGGVAHD
                     FNNVLTAIIGFSDLLLQTHRPTDPAYRDIMNIKSSAHRAAGLVQQLLAFSRRQTLQAE
                     VLELGETLTDLAPLLNRALGEKIELKILPGRDLWYIKADKTQFVQVIINLAVNAKDAM
                     PGGGSLLIRTRNMSERESLKLAELGV"
BASE COUNT          417 a          734 c          781 g          362 t
ORIGIN      
        1 acgcccagtt ccgccaactt gaggctctcg cgctccgaca tattgcgggt gcggatcagg
       61 agcgagccgc cgccgggcat ggcgtccttg gcgttgaccg caaggttgat gatgacctgc
      121 acgaattggg tcttgtcggc cttgatgtac cagaggtcgc ggccaggcag gatcttgagt
      181 tcgatctttt cgcccaaggc ccggttgagc agcggcgcaa gatcggtaag ggtttcgccg
      241 agctccaaca cctccgcctg caacgtttgc cggcgcgaaa aggcgagcag ctgctgcacc
      301 aggcccgccg cccgatgcgc gctgctcttg atgttcatga tatcgcgata ggccggatcg
      361 gtcggccggt gcgtctgcag caggagatcg gagaagccaa tgatggcggt cagcacgtta
      421 ttgaaatcgt gcgcgacacc accggcgagc tgacccaccg cctccatctt gtgactttgc
      481 gcaaacttga gttccagcgc cttctgttcg gtggcatcga cggcgtaaag aatggcgccg
      541 tcgcgctcgc gggcatcgga gctcaaggcg ctgacgtaga cgcgccgcac cagctcgcgc
      601 tcggcgccga aactgatctc gatcggcccg gcggcggcgc gtccggatgc cgctcgccgc
      661 acggccttgc gcaactcgcg cctgacgtct tcgccgccgt tgccgccgag atcggcaatg
      721 ccggtgggca ggccgcgctc atccaggctg aacatgcgca tgaaggcggg attggcgttg
      781 agaatggcgc cgtcagcgtc gaccgtggcc atgccaaacg gggccgagtg caggaaccgc
      841 accagttgcc gctcgctgtc ggcaacgcct gccggctccg cactccggtc gagcacgagc
      901 accgccacgg cgcccttgga gccatgggcg cggcagatca ggtgcgccgg aaagcggcgt
      961 ccatcctcgc gcaacagatc gagatcgaag cggctggtgc ggccgttgcc ggtgcgcccc
     1021 gaggcttgga tgagcgccgc gccgtcgggc gagacgatgt cgcgcagggt cagcgaacgg
     1081 ccagcgtccg ggctcaaatt cagccactgc gccagcgtgg cattgagatg cgcgagccgc
     1141 ccgtccggca acaccgccag caggccctgc ggcaagccgt catagaaggc aagcgtcgat
     1201 tcgagcccgc tgaccgtttc gctctcgcgc atgcgctcgc gcgtcacatc atcgatctgc
     1261 cagagcgtga ggcggcggcg cattttgctc ggcgcgggat cgggaaaggg acgaacggag
     1321 acattgagcc aacggccgga gcgcccgccg agcggccccg gatgcacgta gatttcctct
     1381 tggcgccctt ccaagcgctc ggccgcgcgg ctgagccgga agtaggcttg tgcggtatcc
     1441 ggtgcaccgg cgaacaattc ttccaggctg gcatggcgac cggagcgtcg gccggtcagg
     1501 cgctcgagcg cgcgattgcg ataaagcacg ctgccttggg cgttgacgat ctgcagtgcg
     1561 ctgtccaggc cctcggccac cgcccggatc acgtcggcct cggcggcccg gtcgcccagg
     1621 cgcaggaaac cggacagcag gccgaacacc aagaacacgc cggccaccgc cagcaacgcc
     1681 agcaaaccca ccaccatggg ctcggccatg ctgttggtca gcagcacgag cacgagggca
     1741 acggccagca tcattcccaa cagggaaaat gcgccgccca aggcgtgcac gcgcgcaatg
     1801 gttgtatcgg ccggagaggt ctggcctacc tcgatctctg ccatgctggc gtcttttagc
     1861 tgattctgcg ggagcagcag gcgcagcgcc cgcaagctct ccaccagggc catggacagg
     1921 gtcggacccg ggctcagttc tggacgcata caccaaacca gatggttgtt gatccctcga
     1981 gccggaccac accgattgcg tgtaatgccc aggctcccgc cttcaagaaa gcaatcattc
     2041 aggttaagat gccatgaacg atgcgcgcat tggcgcctgc tctggtgcgg gcgccgttca
     2101 gggcagggga gacttgggcc gacaacgggc gccggggagg acgcgaggcg aggctttggc
     2161 gccggggaga tgggcgcaga gaaactgggc gcagagggac ttggcgcgga gtgacttggc
     2221 gcggagtgac ttggcgcacg gaaagatttg gcgcacggaa agactgagcg agcgtagaac
     2281 gcgggtagaa cctg
//
LOCUS       DASTMT010000049         7746 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_1335331, whole genome shotgun sequence.
ACCESSION   DASTMT010000049 DASTMT010000000
VERSION     DASTMT010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 7746)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7746)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7746
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1335331"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..368)
                     /locus_tag="VFR00_01050"
     CDS             complement(<1..368)
                     /locus_tag="VFR00_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006611509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU0157863.1"
                     /translation="MLSFDHVGKTYPNGVRALDGVSLEVAPGEILVIIGGSGCGKSTL
                     LRAASGLDVATQGRVALDGMTISAPHEKIGLIFQEPRLLPWLRVADNVAFGLSQLPKP
                     ERAKRVALALERVGLTDKAGV"
     gene            complement(380..1216)
                     /locus_tag="VFR00_01055"
     CDS             complement(380..1216)
                     /locus_tag="VFR00_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020187994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0157864.1"
                     /translation="MSTFEILSSPSFVKPHRAAARGGARRLLRPALGLLVPLALALGW
                     EFMVRAGLASGRLVPPPSVIFTTFADLAATGELQRHATVTLLRVLAGFGSGVVLGTLV
                     GAITGYSALSHRLLDPSLQGLRAIPSIAWVPLFILWFGIFEASKIILIAVGVFFPVYL
                     GVMGAVRSVDRKIVEVGRVFRLSGAAMVRRILLPAIAPAYVIALRSGLGLGWMFVVAA
                     EFMGASEGLGYLLIDGQQLGKPAQIVAAIVAFALLGKFTDWIVASVAAPFLRWEDALA
                     SR"
     gene            complement(1291..2277)
                     /locus_tag="VFR00_01060"
     CDS             complement(1291..2277)
                     /locus_tag="VFR00_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012047383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005488 - binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042918 - alkanesulfonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HEU0157865.1"
                     /translation="MWLVGRRGALLAAIVLATFGSALSTASLAADAPKEIRIDWATYN
                     PVSMLLKNKGLLEKAFAKDNIGIVWVQTLGSNKALEFLNAGSIDFGSTAGSAALVAKI
                     NGNPIKSIYVYSRPEWTALVTGKDSAIAKVADLKGKRVAVTRGTDPHIFLVRALQTAG
                     LSEKDIVPVLLQHPDGKTALIRGDVDAWAGLDPMMAQAEILDGAKLFYRKPEANTWGI
                     LNVREGFAKDHPDVVRRVLAVYEEARKEALANYDDLKRVFIAATKLPENVVDKQLKER
                     TELTHSRIGAPQRESILAAGLALQQAGVVPANVDVKATLDALIDDSFLGASH"
     gene            complement(2686..3930)
                     /locus_tag="VFR00_01065"
     CDS             complement(2686..3930)
                     /locus_tag="VFR00_01065"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0157866.1"
                     /translation="MQYQLKPVSPHQVDSRQGWTVTVASLAILSVALGAPYVTIVALK
                     AIAADFGGYRSTPSAAASLAMLGTGVGGLGMGWLADRIGVRAVVSFGAVMVGAGLALA
                     SGGEVWQLYLGHGLLIGLLGNGAINAPLYVYITRWFEQRRGAALALLSGGQYVAGASL
                     PPLFERAIAAYGWQQTMFSYGLVVAVVVLPLALIFLQPAPETAKPGEGPKSTAGHDKV
                     MGLPANLAFSLLAVAAFLCCVPMAMPASHLIALCGDLGFPPSHGPALLTLLLVCAFLS
                     RQFWGWITDRIGGLTTVLVGSLAQATAISGFIVTQSEAGLLGVAAAFGLGFSGLIPAY
                     IVTIRQLFPARDASWRIPTLLLTGMGGMAAGSWLAGRLYDYFGFYAPAFATGLAFNVI
                     NASLIVTLLLFWRSSKRRLALA"
     gene            4034..4702
                     /locus_tag="VFR00_01070"
     CDS             4034..4702
                     /locus_tag="VFR00_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015928269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family oxidoreductase"
                     /protein_id="HEU0157867.1"
                     /translation="MSDPLSIAIYSDVICPWCYVGKRRLEAALASPGMPAPPRFAWLP
                     FELNPDMPAEGVERQAYRANKFGAARSAELDRNMLETGRELGIAFAFDRMTRTPNTRL
                     AHRLIWEAGRQGRQDAMVERLFHAYFEEARDIGEPQTLASLAAEAGLAAEGVAASLGE
                     GESLDAVVDLEQQGYRMGIQGVPFFILARKYAVSGAQPPGFWRDALPRIVAEGAGAQV
                     EAGG"
     gene            complement(5005..6048)
                     /locus_tag="VFR00_01075"
                     /pseudo
     CDS             complement(5005..6048)
                     /locus_tag="VFR00_01075"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(6107..6430)
                     /locus_tag="VFR00_01080"
     CDS             complement(6107..6430)
                     /locus_tag="VFR00_01080"
                     /inference="COORDINATES: protein motif:HMM:NF017128.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF736 domain-containing protein"
                     /protein_id="HEU0157868.1"
                     /translation="MPVIGTFAAVETGYTGSLNTLTLKADVAILPNEHNAGRNAPDFR
                     VMAGAVEVGVAWRRSSLGAQTVVLRVKLDDPTLPEPIWGALLKPGPDGIARLVWRRDW
                     EDERD"
     gene            complement(6443..7675)
                     /locus_tag="VFR00_01085"
     CDS             complement(6443..7675)
                     /locus_tag="VFR00_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008838533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbI/VirB10 family protein"
                     /protein_id="HEU0157869.1"
                     /translation="MNTTPTPPSGSGGARVVPEALALRARPEPVTRINRRVLIGIAAV
                     ILFFLSGLVLAALKPPSLRLGEPRELFSVEHKPISDALAKLPASYAGVRPLKVADATP
                     LPPGVPNLALTRPGPDAAEAAERGPSSRFLAQARESPVLFRLQTHGPATVPRAEAAPR
                     PTAGVEPVGSSLGGSTTPGAAAPGASDADAPREPTRKLAFLKAEPDRAIYNPHALQRP
                     VSPYQLMAGTVIAASLISGLNSDLPGVVLAQVTEPVYDSVTGRTLLIPQGSRLLGRYD
                     NVVAFGQNRALVVWQRLLLPDGSSVLIDNLPATDTSGYAGLADAIDLHTFQLLKGVAL
                     ATLLGVGSELALGSSDNALVKALQQATQQAGNRAGQRLVEQNLNLAPTLTVRPGWPLR
                     VIVHKDIVLKPYVEPNNP"
BASE COUNT         1397 a         2443 c         2786 g         1120 t
ORIGIN      
        1 accccggcct tgtcggtgag gccgacgcgc tcgagcgcga gggcgacgcg cttggcgcgt
       61 tcgggcttgg gaagctggga gaggccgaag gcgacgttgt cggcaaccct gagccaaggc
      121 agcagacgcg gctcctggaa aatcaggccg atcttttcgt gcggggccga gatggtcatc
      181 ccgtcaaggg cgacgcggcc ctgggttgcc acgtcgaggc cgctggcggc ccgcagcagg
      241 gtcgacttgc cacaacccga gccgccgatg atgacgagga tctcacccgg ggcgacctcg
      301 agcgagacgc cgtcgagggc gcgcacgcca ttggggtagg tcttgccgac gtgatcgaag
      361 gagagcatgg acttggtgtt caacgcgagg cgagggcgtc ttcccagcgc aggaaggggg
      421 cggcgacact ggcgacgatc cagtccgtga acttgccaag cagggcaaag gccacgatgg
      481 cggccacgat ctgggcgggc ttgcccagct gctggccgtc gatcagcaaa taaccgaggc
      541 cctcggaggc gcccatgaac tcggccgcca ccacgaacat ccagccgaga cccaagccgg
      601 acctgagcgc aatgacgtag gcgggcgcga tggccggcaa cagaatacgc cgcaccatgg
      661 cggcgccgga gaggcgaaag acgcgaccga cctcgacgat cttgcgatcg accgaacgca
      721 ccgcacccat cacgcccaag tagacgggaa agaacacgcc gacggcgatc aggatgattt
      781 tggaggcctc gaagatgccg aaccacaaga tgaagagcgg cacccaggcg atggagggga
      841 tggcgcgcag gccctgcagg ctgggatcga gcaagcgatg cgacagcgcc gaatagccgg
      901 tgatggcgcc caccagcgtg ccgaggacga cgcctgagcc gaagcccgcg agcacgcgca
      961 gcaaggtgac ggtggcgtgc cgttgcagct caccggtggc ggcgagatcg gcaaaggtgg
     1021 tgaagatcac ggacgggggc ggcaccaggc ggccgctggc gaggccggcg cggaccatga
     1081 actcccagcc caaggccaag gccagcggca cgagcaggcc gagggccggg cgcagcaggc
     1141 gccgcgcgcc gccgcgagcc gccgcgcggt gcggtttgac gaagctcggg gaactcagga
     1201 tttcgaaggt ggacatgagc ggagcgtggc caaacctgga aaaactgtgt tggacgacca
     1261 agccgcgagg cttggtcgcc cacgaaacca tcaatggctg gcgccgagga agctatcgtc
     1321 gatcagggca tcgagcgtgg ccttgacgtc cacattggcc ggcaccacgc ccgcctgctg
     1381 cagcgccaga ccagctgcca gaatggactc gcgctgcggc gcgccgatgc ggctgtgggt
     1441 cagctcggtg cgctctttca gctgcttgtc gacgacgttc tctggcagct tggtggcggc
     1501 aatgaagacg cgcttcagat cgtcgtagtt ggcgagcgcc tctttgcggg cctcctcgta
     1561 gacggcgagc acccggcgca cgacgtcggg gtggtccttg gcaaagcctt cgcgcacgtt
     1621 gaggatgccc caagtgttgg cctcgggttt gcggtagaag agcttggcgc catccaggat
     1681 ctcggcctgc gccatcatgg gatcgagccc cgcccaggcg tcgacgtcgc cgcggatcag
     1741 cgccgtcttg ccgtcgggat gttgcagcag tacggggacg atatcttttt cgctgaggcc
     1801 cgccgtttgc agggcgcgca ccaggaagat gtgggggtcg gtgccgcgcg tgacggcgac
     1861 gcgcttgccc ttgaggtcgg cgaccttggc aatggcgctg tccttgcccg tcaccagggc
     1921 ggtccactcg ggacgcgagt agacgtagat cgacttgatg ggattgccat tgatcttggc
     1981 gaccagggcg gcggagccgg cggtcgagcc gaagtcgatg gagcccgcgt tcaagaactc
     2041 gagtgccttg ttggagccga gcgtctgcac ccagacaata ccgatgttgt ccttggcgaa
     2101 ggccttctcc agcaacccct tgtttttgag gagcatggag acgggattat aggtggccca
     2161 gtcgatgcgg atttcttttg gggcgtccgc ggccagggac gccgtcgaga gggccgagcc
     2221 aaaggtcgcc agcacaatgg cggcgagaag cgcgccgcgt cgcccaacaa gccacatagg
     2281 aatttctccg gctctaggtt tcgagagact gcaataaaag gagctgcggg gctgagaggt
     2341 caggcgcggg gcgcgggaaa ggttccagct tcgaagagac aaagccccac acacgcgcac
     2401 gcacgtttgc aacctcagcc gatcattgcg gggttgacga tcacggcgct tgtcagtcgc
     2461 gccaccttgg cactcctata taaggcaaaa atttcgcttg tgtgcagcgt ggtaaagaaa
     2521 agagcctttt tgcgcgcgca aagctccggc caaaacatat tttgctgcac ttggaaaggc
     2581 tacgatacgg ttttgcgggc tttgcctatt cgagagtggc gcgttcgccg agcgagcgcc
     2641 agcccttcgg agcccgcatc ggctcggcac ggcaagactg ttctctcagg ccagcgcgag
     2701 gcggcgtttc gagctgcgcc agaacagaag caaggtcacg atcaggctag cgttgatgac
     2761 gttgaaagcg aggcccgtgg cgaacgccgg agcatagaag ccgaaatagt cgtagagccg
     2821 gcctgccagc cagctccctg cggccatgcc acccatgccc gtcagcagta gcgtcgggat
     2881 gcgccagctc gcatcgcgag cgggaaagag ctggcgaatg gtgacgatgt aggcggggat
     2941 cagaccgctg aagcccaggc cgaaggcggc ggcgacgccg agcagacccg cttcgctctg
     3001 ggtgacgatg aagccggaga tggcggtggc ctgcgccagc gagccgacca gcacggtggt
     3061 gagcccgccg atgcggtcgg tgatccagcc ccagaactgt cggctcaaaa aggcgcacac
     3121 cagcagcaag gtcaggaggg cgggaccatg cgacggcggg aagcccagat cgccgcagag
     3181 cgcaatcaaa tgcgaggccg gcatggccat cggcacgcag cagagaaagg ccgcgaccgc
     3241 gaggagggag aaggcgagat tggctggcag gcccatgacc ttgtcgtgcc ctgccgtgct
     3301 cttgggcccc tcgccgggct tagcggtctc gggtgccggc tgcaagaaga tgagcgcgag
     3361 cggcaggacg acaaccgcca ccacgagccc atagctgaac atggtctgtt gccagccgta
     3421 ggcggcgatc gcccgctcga ataggggtgg caggctggcg ccggccacgt attggccacc
     3481 cgacagaagc gcgagggctg caccgcggcg ctgctcgaac cagcgcgtaa tgtagacgta
     3541 gagcggggcg ttgatggcgc cattgccgag gagcccgatc agcagcccgt gccccaggta
     3601 gagctgccaa acctcgccgc ccgaggctaa ggcgaggcct gccccgacca tcacggcgcc
     3661 gaagctgacg acggcgcgca cgccgatgcg atcggcgagc cagcccatcc ccaggccgcc
     3721 gacgccagtg ccgagcatgg cgagcgaggc cgccgccgac ggcgtcgagc gatagccccc
     3781 gaaatcggcg gcgatcgcct tcaaggcgac gatggtgacg taaggggcac ccagggccac
     3841 ggacaggatc gcgagcgagg ccacggtgac ggtccagccc tgccggctat cgacctgatg
     3901 cgggctcacg ggtttgagct ggtattgcaa ggctgtccac cgcttggcgt tcctgtcggg
     3961 ggacccgttc tatcgcgctc ccgcgccttg gtctaatgtg ttggcgtcac agcaacgaag
     4021 gaccttgccc ctgatgtccg atcctctctc cattgccatc tattcggacg tgatctgccc
     4081 ctggtgctac gtcggcaagc ggcggctcga ggcggcgctc gccagccccg gcatgcccgc
     4141 gccgccgcgc tttgcctggc ttcccttcga gctcaatccc gacatgcccg ccgagggggt
     4201 cgaacgccag gcttaccggg cgaacaagtt cggggccgcg cgcagcgccg agctcgaccg
     4261 caacatgctg gagacgggac gcgaactcgg catcgccttt gccttcgacc gcatgacgcg
     4321 cacgcccaat acccgcctgg cgcaccgctt gatttgggag gcggggcggc agggccggca
     4381 ggacgccatg gtggagcgcc tgtttcacgc ctacttcgag gaggcgcgcg acattggcga
     4441 gccgcagacg ctggcaagcc tggcggcgga ggccggcctg gctgccgagg gggttgcagc
     4501 ctcgctcggc gaaggagaaa gcctggacgc ggtcgtcgat ctggagcagc agggctaccg
     4561 catgggcatc caaggcgtgc ccttctttat tctcgcaagg aagtacgccg tatcgggtgc
     4621 gcagccgccg ggcttttggc gcgacgccct gcctcggatc gtggccgaag gcgctggggc
     4681 ccaggtggag gccggaggct gaggtctttc gcgctgcgtc agcgagcggg cgtattccag
     4741 gcgcgccgga gccgtggcgg ctgcctccgc gaagccctgg cgcagcatca gggcgaacgc
     4801 gacccgggtg gccatggcca aaccggacgt gcgccgtgac ggtcgtgcgg tcggcccgaa
     4861 aaaccaggcc agggccgcag cttcaagggc cagcacgaca ccgagggcgc gcgcatgcgc
     4921 ctcggccggg tagtgctcgc catggcatgg ccactgctgg atgatgaggc ccatcagcga
     4981 ctgcagggca aaagcgccgc cgacatggca taggttcaag gcggcattgg cccgtcccga
     5041 gacttcgcgc ggatagctat cggccaggat ggcgtagctg agcaccgtgg cggccccggc
     5101 cgcggcgaca gcactccagg ccaaagcggc gggcagcggt agctggaggt agaggccggc
     5161 ctgggcggcg atcgacaggc cgacgacccc caacagcacg tgctcggtct tgccgccgag
     5221 gcggcgcacg tggttggcga tcaggccaag cgccaagccg ctggcgctga cgacgatcgc
     5281 catcaggctc aacaccccga ccacatcggc gcgttcgaga ccgtcaacgt ctctcagcca
     5341 gggtgccgcc cacagcccct gcagcgacca ggaggtgccg acgccgagag aggagagcgg
     5401 ggccaggcgc caaaagcgcc gttgccgata aatcgcggcc aatgtgaggg agggttgcgc
     5461 acccgcgggg ggcttagtgg gccgatcctg atcggggacg aggacgacga tcaggagtgc
     5521 ggccaaggcg ctcaaggcgc cgagcacggc aaaaagcccg cgccagccga cgtggccgac
     5581 taggagttcg gcgggcgcgg cggcggtgac ggcgcctagc gcgcccagca tgacgaacca
     5641 gccgttggtg agcgccgggc gctcggccgg aaaccagagt acgatcgcct tgaagccgcc
     5701 catcagggcg agggcgacgc ccaagccgat cagcgcgcgt ccggcgatga gacccaatag
     5761 cgtgtcggcg agcgcgaaca gaagcgatcc cgcgctggcc agcaacagca gccccgccaa
     5821 gagcgtgcgg gggccgtggc ggtcgagcaa gatgccgaag gggatctgtg ccgcggcgaa
     5881 caccagaaaa tagaccgacg tcagcagtcc gaggtcggcc gccgagaggc tcaagtccgc
     5941 cgtcaagtcg gcggcgatca aggcgttgat ggtgcgaaag agataggaca gatagtagcc
     6001 ggctgcgaag ggcaacaaga cgcagccgat ccggcgtgat cgggtcacgg gcttccctcc
     6061 ctgagatttt ttgtttttga cgttcggcgc ggctgggcgg cctggctcag tcgcgctcgt
     6121 cctcccaatc ccgccgccac accaggcggg cgatgccgtc tgggccaggc ttgagcaagg
     6181 ccccccagat cggctcgggc aaggtcgggt cgtcgagttt gacgcgcagg accacggttt
     6241 gagcgccgag actgcttcgg cgccaggcca caccgacctc gaccgctccc gccatcaccc
     6301 ggaaatccgg cgcgttgcgc ccggcgttgt gctcattggg caggatcgcg acgtcggcct
     6361 tgagggtgag cgtgttgagg ctgccggtat agccggtctc gacggctgca aacgtgccaa
     6421 tcacgggcat ggcggtcctc cactaggggt tgttaggctc gacgtagggt ttcaggacga
     6481 tgtccttgtg cacgatcacg cgcagcggcc agcccgggcg cacggtgagg gtcggcgcga
     6541 gattgaggtt ctgctcgacc aggcgctggc ccgcgcggtt gccggcctgt tgcgttgcct
     6601 gctgcagcgc cttgacgagg gcgttgtcgg aggatcccag cgccagctcg ctccccacgc
     6661 ccagaagggt ggcgagtgct acgcccttca ggagctggaa ggtgtggagg tcgatggcat
     6721 cggcgagccc cgcatagcca ctggtgtcgg tcgccggcag gttatcgatc aggaccgagc
     6781 tgccatcggg caggagcagg cgttgccaga cgacgagggc gcggttttgg ccaaaggcga
     6841 cgacattgtc atagcgtccc agcagccggg agccctgcgg gatcaacaag gtgcgcccgg
     6901 tgacgctgtc gtagacgggc tcggtcacct gggcgaggac aacgccgggc agatcggagt
     6961 tgagaccgct gatcaggctg gcggcgatga cggtgccggc catcagctgg tagggcgaga
     7021 ccggccgctg cagcgcgtgc ggattataaa tggcgcggtc gggctcggcc ttgaggaacg
     7081 ccagtttgcg ggtgggctcc cgcggggcat ctgcatccga cgcccccggg gcggccgcgc
     7141 cgggcgtcgt cgacccacct aaagacgaac caacaggctc gacacctgct gtcggtcgcg
     7201 gcgccgcctc ggcgcgcggc accgtcgcgg gcccatgggt ttgcaggcga aagagcacgg
     7261 gcgactcgcg ggcttgcgcc aggaaacgcg agctcgggcc acgctccgcg gcctcggccg
     7321 catcggggcc tggacgtgtc aaggcgagat tgggcacgcc cggcggcagc ggcgtcgcgt
     7381 cggccacctt gaggggccgc acgccggcgt agctcgccgg cagcttggca agcgcgtcgc
     7441 tgatcggctt gtgctcgacc gagaagagtt cgcgcggctc gccgaggcgt aggctcggtg
     7501 gtttcagggc ggcgagcacc agccccgata gaaagaataa aatgaccgcg gcgatgccga
     7561 tcagcacgcg ccggttgatg cgggtgacgg gctcgggccg ggcccgcaag gccagggcct
     7621 ccggcacaac cctggccccg ccgctgccgc tgggaggggt tggcgtcgtg ttcatggctg
     7681 cacctcccgc cagacggcgt cggtgcggat gatgcgcacc acgcgctgcg gtgccgtccc
     7741 gagccg
//
LOCUS       DASTMT010000050         4021 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_4934648, whole genome shotgun sequence.
ACCESSION   DASTMT010000050 DASTMT010000000
VERSION     DASTMT010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4021)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4021
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4934648"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..726
                     /locus_tag="VFR00_01090"
     CDS             <1..726
                     /locus_tag="VFR00_01090"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF026183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU0157870.1"
                     /translation="NGLIAGMATGSAWFDLTTNSPTLVRRLHQVCSERGLHLLDAPVS
                     GGPKGAKSGRLAVWVGGEEAIFNANRAVLDAFSDAARYIGPIGAGSVAKLVHNCAGYA
                     IQMALAEVFSLGVKAGVEPLALWEAVRTGASGRQRTFDRLHDRFLPNKYDPPSFALKL
                     GHKDMRLATELGRELGVPMRLANMAYAEMTEALNRGWENRDSRSPMLLQLERCGLKIE
                     VDPQRIQEIFDRDPPHNGARTRG"
     gene            complement(829..2136)
                     /locus_tag="VFR00_01095"
     CDS             complement(829..2136)
                     /locus_tag="VFR00_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011652433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxide hydrolase"
                     /protein_id="HEU0157871.1"
                     /translation="MSRTSSSPSRRQLLSAASMGLALAGAASLVPGRPRAAVTPDTIR
                     PFRVNVPEQDLEDLRRRIAATRWPDKETVADQSQGAPLLGLQQLVAYWGSGYDWRKVE
                     ATLNALPQFTTMIDGVDIHFIQVRSRHQRALPVIITHGWPGSIIEQLKIIGPLTDPTA
                     HAGRSEDAFDVVIPSLPGYGFSGKPTGPGWDPDHIARAWAELMKRLGYTRYVAQGGDW
                     GAPISSAMARQAATGLLGIHINLPATIPPEVAAALAGGPLPAGVSEQERAVIEALMAN
                     AKNGNAAYFTMMTARPQTVGYGATDSPAGLAAWMLVHPGFAQWRFGADPGTVPSKDEV
                     LDNITLYWLTNTATSAARLYWENGARGSVIVAAAQKTGEIALPVAISVFPDDVYRAPE
                     SWARRAYRNLIYFREVDRGGHFAAWEQPELFSSELRAAFRSLR"
     gene            complement(2204..2629)
                     /locus_tag="VFR00_01100"
     CDS             complement(2204..2629)
                     /locus_tag="VFR00_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018646118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEU0157872.1"
                     /translation="MSARSSAPTRRRVLAASAAAVVGSGLRSKPARAQQVGSKRTDLQ
                     RHDLSIPGREVIQVVVELAPGVAAPAHSHPGEEIIYVIEGLWEYQLEGKPPVTLKAGE
                     VLFIPAGAIHSAKNVGSSHGAELATYIVEKGKPLLMLAK"
     gene            complement(3086..>4021)
                     /locus_tag="VFR00_01105"
     CDS             complement(3086..>4021)
                     /locus_tag="VFR00_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019198553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU0157873.1"
                     /translation="QLASSDVIRPVLGVEPIETTRKLAETAGLETTARFEDALERPDI
                     GAIILATPHKHHAAQIIAAAKAGKHVFCEKPLCTTAQEIEAVIDAVTAARVELGIGHE
                     RRFEPGIIDLRARLAAGELGTALAMEGNFSQDKFLALSPNNWRLSSVDAPVGPLSATG
                     IHLVDLSIAVLGKPVEVWARLATRGSPFANGDTLGVMIAFESGASALLSAILATSFMG
                     RIAVYGSQGWIEIRDRTHPEHPTGWDVTIAARGKAPATHALPPHPAVRDNLEAFGRAA
                     AGAAPYPVSLAEIDTNVRTFAAITRSALSGKLERV"
BASE COUNT          686 a         1347 c         1328 g          660 t
ORIGIN      
        1 aacgggctga tcgcgggcat ggcgacggga agcgcctggt tcgatctcac caccaactca
       61 ccgacgctgg tgcgcaggct gcaccaggtc tgtagcgagc ggggcttgca cctgctcgac
      121 gctccggtca gcggcggccc caagggcgcc aagtcaggtc ggcttgccgt ttgggtcggg
      181 ggcgaggagg caatctttaa tgccaacaga gccgtgctcg acgccttcag cgacgccgcg
      241 cgctacatcg gcccaatcgg cgccggctcg gtggccaagc ttgtgcacaa ctgcgccggc
      301 tatgccattc aaatggcgct ggcggaggtc ttctcgctcg gggtcaaagc cggggtcgag
      361 ccgctcgccc tgtgggaagc cgtgcgcacc ggcgccagcg gtcgccagcg caccttcgac
      421 cgtcttcatg accgctttct gccgaacaag tacgaccctc caagctttgc cttgaagctt
      481 gggcacaagg acatgcggct ggccaccgag ctcggccgcg aactgggcgt gccgatgcgg
      541 ctcgccaaca tggcctacgc cgaaatgacg gaggccctca accgcggctg ggagaaccgt
      601 gacagccgct cccccatgct gcttcagctc gagcgctgtg gcctcaaaat cgaggtcgat
      661 ccacagcgca ttcaagagat ttttgatcgt gacccgccgc acaatggcgc tcggacgcgc
      721 ggttgaggcc tgacacctcg caccctcgct gggcgcgcgc cgtgacgctc gccagtggtg
      781 cgcccttttt gggcgggcat cgatcatgcc cgcctcgcac ggagccactc agcgcagcga
      841 tcggaaggcg gcacgaagct ccgaggagaa cagctccggt tgctcccagg ccgcgaagtg
      901 accgcccctg tcaacctcgc ggaaataaat gagattgcgg taggcacgcc gggcccagct
      961 ctcgggcgcg cgatagacgt cgtccggaaa gacgctgatt gccacaggca gcgcgatctc
     1021 gccggtcttc tgggccgccg ccacgatgac gctgccccgc gcgccgttct cccagtacaa
     1081 gcgcgccgcc gaggtggccg tgttcgtcaa ccaatagagc gtgatgttgt ccaaaacctc
     1141 gtctttgctc ggcactgtgc cgggatcggc gccaaacctc cactgtgcaa agcccggatg
     1201 gaccagcatc caggcggcga ggccggcggg cgagtccgtc gcaccgtagc cgaccgtctg
     1261 cggacgcgcg gtcatcatgg tgaagtaggc cgcgttgcca ttcttggcgt tggccatcag
     1321 cgcttcgatc accgcgcgtt cctgctctga aacgcccgcc ggcagcggcc cgcccgccag
     1381 cgcggcggcc acctcgggcg gtatcgtcgc cggcaggttg atgtggatac cgagcaaacc
     1441 cgtggccgct tgccgcgcca tggcgctgga aatgggagca ccccagtctc cgccctgggc
     1501 cacatagcgc gtgtagccga gacgcttcat caattccgcc caggcgcgcg cgatgtggtc
     1561 gggatcccaa ccggggccgg tcggcttgcc ggagaaaccg taaccaggca gggacggtat
     1621 gacgacatcg aaggcatcct cgctgcgccc cgcatgcgcg gtcggatccg tcagcggccc
     1681 gataatcttg agttgctcga tgatggagcc cggccagccg tgcgtgatga tcaccggcag
     1741 cgcacgctga tgccgggagc ggacctggat gaagtgaata tcgaccccgt caatcatggt
     1801 cgtgaactgc ggcaaagcat tgagggtagc ttccaccttg cgccagtcgt aaccgctccc
     1861 ccaataggca accagctgct gcagtccgag caagggggcg ccttgcgact gatcggctac
     1921 ggtttcctta tcgggccagc gcgtggcggc gatgcgccgg cgcaagtcct cgaggtcttg
     1981 ctcgggaaca ttgacgcgaa aggggcggat tgtgtcaggc gtgacagccg cccgcggacg
     2041 cccaggaaca aggctcgcgg caccggctag cgccaatccc atgctggcgg cgctcaggag
     2101 ctggcgccgg ctcggtgacg aggacgtcct agacatgacc ggtctccttg cgggcatgcg
     2161 agccggcccg atcggcacgc atcatcggcg ccaagccatc ccctcacttt gccagcatca
     2221 ggagcggctt gcctttttcg acgatatagg tggcaagctc tgcgccatgg ctgctgccga
     2281 cgttcttcgc cgaatggatc gctccggccg gaatgaacaa tacctcgcca gccttcagcg
     2341 ttaccggcgg cttgccctcc agctgatatt cccacaggcc ctcgatgaca tagataatct
     2401 cctcaccggg atgcgagtgc gcaggtgccg ccactcccgg cgccagctcg acgaccactt
     2461 ggatgacctc gcgtccgggg atgctgaggt cgtgccgctg taggtcggtg cgcttgcttc
     2521 ccacctgttg ggccctcgcc ggctttgacc tcaagccgct gccgacgacg gccgcggcag
     2581 aggctgcaag aacacggcgc cgggtcggtg cggaggaacg cgcggacatg accagtctcc
     2641 tggatagcgg gtgggcccca agcggctggc attgacgagc tcgcacccgc ggcaatgctg
     2701 gcagggggct tgaccccgat cgcatgggcg atcggcgcac gccgcagcca cggcgccaga
     2761 gcggtcggcg cggtcgtgct ggaacgaccg ccagcgcccg ccgttagcgc cgatgccgcc
     2821 aaccctaggt ggcggccgcc caaatgcact agcggtgaaa gcactgagac cggcgagcac
     2881 ttgacctgat gtcggagcga tgccgtcgac ccgaccgcgc gcggacaaga tggctcgggc
     2941 tcgcttaccc gccacggccc gttcagccaa gcgccccgcc aggtcagcag agccgtccgt
     3001 cgactgacgg gtgcaagcaa agacgagacc tgaggcgcgc cggacgtgcg gcggagctcg
     3061 ctttttgccg ccaatcaggc ctggctcaga cccgctccag cttaccgctc agtgccgaac
     3121 gcgtaatggc ggcgaaggtg cgcacattgg tgtcgatctc ggcgaggctc accgggtagg
     3181 gcgcagctcc ggccgcggcg cgtccgaagg cctccagatt gtcccttacc gccggatgcg
     3241 gtggcagggc atgcgtcgcc ggggctttgc cgcgagccgc gatcgtgacg tcccatcccg
     3301 tgggatgctc gggatgggtg cggtcgcgga tctcgatcca accttgcgag ccgtaaacgg
     3361 cgatgcgacc catgaaagag gttgccagaa ttgcgctcaa tagagcgctg gcgcccgact
     3421 cgaaggcgat catgacgccc agcgtatcac cgttggcaaa cgggctgccg cgcgtcgcca
     3481 gccgtgccca cacctccacc ggtttgccca ggacggcaat cgagagatcg acgagatgaa
     3541 taccggtcgc cgacagcggc ccgaccggag catccacgga tgagaggcgc caattattcg
     3601 gggaaagcgc gaggaacttg tcctggctga agttgccttc catggccaag gcggtaccga
     3661 gctcgccggc ggcgagcctg gcccgcagat cgatgatgcc cggctcgaag cgccgctcgt
     3721 ggccgatgcc gagctcaacc ctggccgccg tgacagcgtc gatgaccgcc tcgatttcct
     3781 gcgccgtggt gcaaagtggc ttttcgcaga atacgtgctt gccggccttg gccgccgcta
     3841 tgatctgcgc ggcgtgatgc ttgtgtggcg tcgccaggat aatagcgccg atgtcggggc
     3901 gttcgagcgc gtcctcgaag cgggccgtcg tctccagccc ggccgtttcg gccagcttgc
     3961 gcgtcgtctc gatcggctca acgccgagca ccggccggat gacgtcgcta gaggcaagct
     4021 g
//
LOCUS       DASTMT010000051         2170 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_2322316, whole genome shotgun sequence.
ACCESSION   DASTMT010000051 DASTMT010000000
VERSION     DASTMT010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2170)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2170
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2322316"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1011)
                     /locus_tag="VFR00_01110"
     CDS             complement(<1..1011)
                     /locus_tag="VFR00_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009736476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEU0157874.1"
                     /translation="MSATAELPSPSPKPARFSDASAARAAALAFDLRHLPGDYLINPY
                     PYYHALREYAPVHLLPDGGYFVSRYADCAAVYKDVVLFSSDKKSEFKPKYGDSLLYQH
                     HTTSLVFNDPPLHTRVRRIIAGAFTPRALGALEASVITLVDGLLDRLGQAGGGDLIGD
                     FAAAVPVEVIGNLLDVPREERAPLREWSLAILGALEPTLTAEQHGRGEQAVRDFLDYL
                     KGLVARRRAKLGDPEHDVLTRLIAGERGGEELSEPELLHNIIFILNAGHETTTNLIGN
                     GLYLLLEWPDARAKLIGNPALIRPAVEEILRFESSNQLGNRLTTAPSDIGGVRLPAGT
                     FLT"
     gene            complement(1185..>2170)
                     /locus_tag="VFR00_01115"
     CDS             complement(1185..>2170)
                     /locus_tag="VFR00_01115"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEU0157875.1"
                     /translation="NISTNAGGIQVLRYGNMRALVMGLEVVLPDGRIWDGLRGLRKDN
                     SGYDMKQIFIGAEGTLGIITGAVLRLFPKPTAAETAFVAVDSPQAGVGLLRHMLARMG
                     DTISAFELIGRQIIDFLLAGVPGHQDPMHETHPWYVLVNVANQGAPSSLLAPLSDALA
                     EASEVGLIRDALIAASGAQTARLWKIRESLVEAQAAAGGTIAHDVSVAVSRIAEFLHR
                     ADAALSEAYPGVRPCAFGHVGDGNMHYNVVRPPDWDAARFRAERGNINRIVHDIIADL
                     GGSISAEHGIGRARLGELEHYKAATELAMMRALKQALDPQGIMNPGKVLQA"
BASE COUNT          381 a          694 c          723 g          372 t
ORIGIN      
        1 ggtcaggaag gtgccggcgg gcagcctcac cccgccgatg tcgctgggcg ccgtcgtcag
       61 gcggttgccg agctgatttg agctttcgaa gcgcaggatt tcttccaccg ccggccggat
      121 cagcgccggg ttgccgatca gtttggccct ggcatctggc cactccagca ggagatagag
      181 gccgttgccg atgagattgg tggtcgtctc gtggccggca ttgagaataa agatgatgtt
      241 gtgcagtagc tcgggttcgg aaagctcctc gccgccccgc tcgccggcaa tgagccgggt
      301 cagcacgtca tgctccgggt cgccgagctt ggcgcggcgc ctggcaacga ggcccttcag
      361 gtagtcgagg aagtcacgca cggcctgttc gccgcgcccg tgctgctcgg ccgtcaacgt
      421 gggttccagg gcaccaagga ttgcaagcga ccactccctt aagggagcgc gctcctctcg
      481 cggcacgtcc aacagattgc cgatcacctc aaccggcacc gcagccgcga agtcgccgat
      541 aagatcgcct ccgccagcct gacccaaccg atcgagcagg ccatcgacca gcgtaatcac
      601 cgaggcttcg agggcgccga gggcgcgcgg cgtgaaggcg ccagcgatga tgcgacggac
      661 ccgcgtatgc agcggtggat cattgaagac aaggctcgtg gtgtggtgct ggtagagcag
      721 gctgtcgccg tatttgggtt tgaactcgct ttttttgtcg gatgaaaaca acaccacatc
      781 tttgtagacg gcggcgcagt cggcgtaacg cgatacgaaa tagcccccat cgggcagcag
      841 gtggaccgga gcgtactccc ggagcgcatg atagtagggg tagggattga tcaagtagtc
      901 accgggtagg tgccgcaaat cgaaggccaa ggctgccgcg cgtgcggcgc tggcatcgct
      961 aaagcgagcc ggtttggggc tggggctcgg cagctcggcc gtcgcgctca tggcactgac
     1021 ttcctgcacg gtcgctcgca atgcatgacc gccaatcctg ccaaattgct agcgccctag
     1081 cgcggggccg ggcacgtata gggtagcggc cgcgccgttg ttgaggcaag cggcaagcgc
     1141 cggctcgcgc ccatgcccag cccagcgagg cgcatcggtt tccctcaggc ctgcaagacc
     1201 ttgccggggt tcatgatccc ctgcggatcg agcgcctgct tgagggcccg catcatggca
     1261 agttccgtcg cggccttgta gtgctcgagc tcgccgaggc gcgcgcggcc gatgccatgc
     1321 tcggcgctga tcgaaccacc gagatcggcg atgatgtcgt gcacgatgcg gttgatgttg
     1381 ccgcgctccg cacgaaagcg cgccgcgtcc cagtcgggcg ggcgtaccac gttgtagtgc
     1441 atgttgccat cgcccacatg gccgaaggca caggggcgga cgccgggata ggcctcgctc
     1501 agcgccgcat cggcccggtg gaggaactcg gcgatgcgcg agacggcgac cgacacatcg
     1561 tgcgcgatcg tgccccccgc ggcagcctgc gcctccacca ggctttcgcg tatcttccac
     1621 aatcgcgcgg tttgcgctcc ggaagcggca atcagcgcgt cgcggatgag gcccacctcg
     1681 ctcgcctcgg ccagagcatc gcttaagggc gcgagcaggg agctcggcgc gccctgattg
     1741 gcgacattga cgagcacgta ccaaggatgc gtctcgtgca tcgggtcttg atggccgggc
     1801 acgcccgcca gcaggaagtc gataatttga cggccgatga gctcgaaggc ggagatggtg
     1861 tcgcccatgc gggccagcat gtgccgcagc aagccgacac cggcctgcgg gctgtcgacg
     1921 gccacgaagg cggtctccgc ggccgtcggt tttggaaaca gccggagcac cgcgccggtg
     1981 atgatgccca gcgtaccctc cgcgccgatg aagatttgct tcatgtcgta accggagttg
     2041 tccttgcgca agccacgtag cccgtcccag atgcgtccgt ccggcagcac cacctcgaga
     2101 cccatcacca acgcccgcat attgccgtag cgcaaaacct gaatgccgcc cgcattggtc
     2161 gaaatgttgc
//
LOCUS       DASTMT010000052         2236 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_5264192, whole genome shotgun sequence.
ACCESSION   DASTMT010000052 DASTMT010000000
VERSION     DASTMT010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2236
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5264192"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            17..1225
                     /locus_tag="VFR00_01120"
     CDS             17..1225
                     /locus_tag="VFR00_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019404349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FCSD flavin-binding domain-containing protein"
                     /protein_id="HEU0157876.1"
                     /translation="MCAPALAQAAPRVLVIGGGFGGATAARAIKQASPRVSVSLVERE
                     RTFTACPFSNLVISGLRALPAQQFGYDKVSAAGVDVIFAAATGVNREARRVELEGGAS
                     LPYDRLIVAPGIDIRWDGLTGYNEAAAAKLPHAWKAGEQTLLLRRQLEALEDGGVVVM
                     SVPANPFRCPPGPYERASLIARYLKTQKPKSKILILDAKDTFSKQRLFQEAWKELYPD
                     HLEWVALSKGGRVTAVDAETKTFTTEFGEYKAAVGNVIPPQRAGRAAELAGAVDQTGW
                     CAIDPVTFESKLSPNVHVVGDATIAGGMPKSAFSANAQAKVCAAAVVELLEGRTPKPP
                     KLINTCYSLVAPNYGISVAGVYEPVNGVLTDVPGAGGVSPAAAPRSERSQEALYAEAW
                     FNTVTAEIFG"
     gene            1225..1689
                     /gene="soxX"
                     /locus_tag="VFR00_01125"
     CDS             1225..1689
                     /gene="soxX"
                     /locus_tag="VFR00_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018457156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur oxidation c-type cytochrome SoxX"
                     /protein_id="HEU0157877.1"
                     /translation="MGVPHRLARAGVLAGILSLSPAALGAEGPLKSFSVTGDGIAASL
                     TGAPGDSVRGRGIVVNRQVGLCLLCHSGPFPEERLQGNLAPDLKGAGARWSEAQLRLR
                     IVDAGVINPNTIMPPYYRVDGLERVAPTFRGKPILTAEQIEDVVAFLVTLKE"
     gene            1764..2207
                     /locus_tag="VFR00_01130"
     CDS             1764..2207
                     /locus_tag="VFR00_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008967224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxY-related AACIE arm protein"
                     /protein_id="HEU0157878.1"
                     /translation="MRFAGALSLGSLLPCLAVRPAGATPASVKAAIATVIGDAKVNSG
                     RVKLEIPPLVENGNTVACSVAVESPMTETDYVKAIHIFNEKNPQPNVISVKLGPRAGR
                     ALIATRIRLSDSQTVTAIAQMSDGSFWSTSVDVLITLGACLEDPL"
BASE COUNT          366 a          728 c          753 g          389 t
ORIGIN      
        1 ggcggcggtt gcgggtgtgt gcgcgcccgc cctggcgcag gccgcgccca gggtcctggt
       61 gatcggcggc ggtttcggcg gtgccacggc ggcgcgcgcc atcaagcagg cttccccgcg
      121 cgtgagcgtg agcttggtcg aacgggagcg cacgtttacg gcctgtccct tcagcaacct
      181 ggtgatcagc ggcttgcggg ccttgccggc ccagcagttt ggctacgaca aggtcagtgc
      241 cgcgggcgtc gacgtcattt tcgcggcggc gacgggggtc aatcgcgagg cgcgccgggt
      301 cgaactcgag ggcggggcga gcctgcctta cgaccggctg attgtcgccc ccggtatcga
      361 catccgctgg gatggcctta ccggctataa cgaggcggcg gccgccaagc tgccgcacgc
      421 ctggaaagcc ggcgagcaga cgcttttgct gcgtcgccag ctcgaggccc tcgaggacgg
      481 cggtgtggtc gtcatgtcgg ttccggccaa tcccttccgc tgtccgcccg gaccctacga
      541 gcgcgccagc ctgattgcgc gttatctcaa gacgcaaaaa cccaaatcga aaatcctgat
      601 cctcgatgcc aaggatacct tttccaagca gcgcctgttt caggaggctt ggaaggagct
      661 ctaccccgac cacctcgagt gggtggcact ctccaagggt ggtcgtgtca cggccgtcga
      721 tgcggaaacg aagaccttca cgacggaatt cggcgagtac aaggccgccg ttggcaacgt
      781 tataccgccg caacgggcgg ggcgcgcggc cgagcttgcc ggcgccgtcg atcagacggg
      841 atggtgcgcg atcgatcccg tcaccttcga atccaagttg agcccgaacg tccacgtggt
      901 gggcgatgcg acgattgccg gtggcatgcc gaagtcggcc ttttccgcca acgcgcaggc
      961 caaggtttgc gctgccgccg tggtggaact gctggagggc cgcacaccca agccgccgaa
     1021 attgatcaat acctgctaca gcctggtcgc ccccaactat ggcatcagcg ttgccggggt
     1081 gtatgagccc gtgaatgggg ttttgaccga tgttcccggc gccgggggtg tgagccccgc
     1141 ggcggcgcca cgctcggagc gctcgcaaga ggcgctctac gcggaggcct ggttcaacac
     1201 cgttacggcc gagatcttcg gttgattggg gtcccgcaca ggctggcgcg cgccggcgtg
     1261 ctggccggca tcctcagcct gagccccgcc gccctcggcg ccgaggggcc gctcaagagc
     1321 ttcagcgtca ccggcgatgg catcgcggcc tccctgaccg gtgcgcccgg cgatagcgtg
     1381 cgcgggcgcg gcatcgtcgt caaccgtcag gtcggccttt gcctgctctg ccactccgga
     1441 ccatttcccg aggagcggtt acagggcaac ctggcgccgg atctcaaagg cgctggcgcg
     1501 cgttggtctg aggcgcagct gcggctcagg atcgtcgatg ccggcgtcat caatcccaac
     1561 accatcatgc ctccttatta tcgtgttgac ggtcttgagc gcgtcgcccc cacgtttcgc
     1621 ggcaagccga tattgacggc ggagcagatc gaggatgtgg tggcatttct agtgaccctg
     1681 aaggagtaag ccgagcgatg ggcgagagcg gcagggacaa acgtcgcgcc ggggctcagt
     1741 tgccaagcac gcggcgcgag gtcatgaggt ttgcgggtgc cctcagcctc ggcagcctgc
     1801 tgccgtgcct cgcggtgcgc cccgcagggg cgaccccggc cagcgtaaaa gcggcgatcg
     1861 ccacggtgat cggcgatgcc aaggtgaact cggggcgcgt caagctcgag atcccgccgc
     1921 tggtcgagaa cggcaacacg gtggcttgtt cggtggcggt cgagagcccg atgacggaga
     1981 ccgactatgt caaagccatc cacattttca atgagaagaa cccgcaaccc aacgtgatca
     2041 gcgtcaaact gggcccgcgc gccggccgcg cgctcatcgc cacccgcatc cgcttgtccg
     2101 actcgcaaac cgtgaccgcc atcgcccaga tgtcagatgg ttcgttttgg tcgacgagcg
     2161 tcgacgtcct catcacgctg ggtgcctgcc tggaggatcc gctctaatgg cgcgctctct
     2221 catcaaggta tcgccg
//
LOCUS       DASTMT010000053         2171 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_1010414, whole genome shotgun sequence.
ACCESSION   DASTMT010000053 DASTMT010000000
VERSION     DASTMT010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2171)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2171)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2171
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1010414"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..455
                     /locus_tag="VFR00_01135"
     CDS             <1..455
                     /locus_tag="VFR00_01135"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HEU0157879.1"
                     /translation="PTASNSLPARIIFVRSKQAKERLRPHLSEGNRERTMLAPACAII
                     GYHAKFYDHPPKGQDPLKGFAQDPAGAVTAAMRNSSLQGGYFVLAARALGLDAGPMSG
                     FDPPGVDQEFFAGTEVKSNFLCNLGYGDPPKLRPRGPRFPFAEMASVI"
     gene            461..1171
                     /gene="tsaB"
                     /locus_tag="VFR00_01140"
     CDS             461..1171
                     /gene="tsaB"
                     /locus_tag="VFR00_01140"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEU0157880.1"
                     /translation="MHILAFDTCFDALSVAVARAQAQDVHVLLERSEPRRPGHAERLL
                     PLVVEALEALNLSFAEVGRIAVTRGPGSFTGVRTGVAAARALALAIGCPAVGVECLAA
                     IAPDARAQLGAVAAGRPLGVALDARSGLVYFQLFEATGEAREGALLLSAPACAERLGQ
                     APAIVVGSAAALVAQAAAALGGHAEARLPEAEPRAGWVARLGATLAPGEPLKPLYLRT
                     PDAKPQTGYALPRIPPRC"
     gene            1165..1638
                     /locus_tag="VFR00_01145"
     CDS             1165..1638
                     /locus_tag="VFR00_01145"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU0157881.1"
                     /translation="MLAAHDGRVSLRRATPADAQDCATLHAQLFEPAWDRASFASMLS
                     HPAALGFVAHRLAQAQSVGFILGQVAADEAEVLTLAINTEARRQGIATRLLDALAEAA
                     GAAGACVLHLEVAVDNAAALALYRKLRFVERGRREGYYVGAGAGPVDAVTFARAL"
     gene            1723..2163
                     /locus_tag="VFR00_01150"
     CDS             1723..2163
                     /locus_tag="VFR00_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012112473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HEU0157882.1"
                     /translation="MSVLIETFPSRIERLCAEKQLRLTGQRRIIARVLSDSKDHPHVE
                     EVYRRANAVDSRISLSTVYRTLKLFAAKGILERHDFGAGRGRYEEAAGKHHDHLIDIE
                     TGKVIEFRNEEIERLQERVARELGFTLVGHRLELFGVPLKRVRK"
BASE COUNT          323 a          752 c          735 g          361 t
ORIGIN      
        1 gcccgacggc ctccaattcg ctgcccgcgc gcatcatctt cgtgcgttcc aaacaggcca
       61 aggaacgcct gcggcctcac ctcagcgaag gcaaccggga gcgcaccatg ctggcgccgg
      121 cctgtgccat cattggctat cacgccaaat tctatgacca tccccccaag gggcaggacc
      181 ccctcaaggg cttcgcccag gaccccgcgg gcgccgtcac cgcggccatg cgcaacagct
      241 cgctgcaggg cggctacttc gttctggccg cgcgggcgct gggcctcgat gccggcccga
      301 tgtcgggctt cgacccgccg ggcgtcgatc aggaattttt cgccggcacc gaggtgaaat
      361 cgaatttctt gtgcaacctc ggctatggcg atccgccgaa actgcggccg cgcgggccgc
      421 gcttcccctt cgcggagatg gcgagcgtca tttagaggcg atgcacatcc ttgccttcga
      481 tacctgtttt gacgcgctct ccgtggccgt ggcgcgggcg caagcgcagg atgtgcacgt
      541 cttgctggaa aggtcggagc cgcggcgccc gggccacgcc gaacggctgc tgccgctggt
      601 ggtggaggcg ctcgaggcgc tcaacctcag ctttgcggag gtcgggcgca ttgcggtcac
      661 gcgcggtccg ggcagcttca cgggcgtgcg caccggcgtt gccgccgccc gcgctctggc
      721 actggcgatt ggctgccccg ccgtcggcgt cgaatgtctg gcggcaatcg ccccagacgc
      781 ccgggcacag ctcggcgcag tggccgccgg ccgcccgctc ggggttgcgc tcgatgcccg
      841 ctcggggctg gtctatttcc agctgttcga ggccacgggc gaagcgcgcg agggagcgct
      901 gctgctttcc gcgcccgcct gcgccgagag gctggggcag gcgcccgcca tcgtcgttgg
      961 ctcggcggca gcgctcgttg cgcaagccgc ggcagcgctg ggcggccacg ccgaggcgcg
     1021 cctgcccgag gcggagccgc gcgccggctg ggtcgcccgg ctcggcgcca cgctggcccc
     1081 cggagagccg ctgaagcctc tctatttgcg gacgcccgac gccaaacctc agaccggcta
     1141 cgccctgccg cggatccctc cccgatgcta gccgcacacg acgggcgcgt gagcctgcgc
     1201 cgcgccacgc cggccgacgc ccaggactgc gccacgctgc acgctcaact gttcgagccg
     1261 gcctgggatc gcgcaagttt tgcaagcatg ctcagccatc ccgccgcgct ggggttcgtg
     1321 gcgcaccggt tagcgcaggc gcaaagcgtc ggcttcattc tgggccaggt ggcggccgat
     1381 gaggcggagg tcctgacgct cgcgattaac accgaggcgc gccgccaggg cattgccacg
     1441 cggctgctcg acgccctggc cgaggccgcc ggcgcggccg gtgcatgcgt gctccatctg
     1501 gaggtggccg ttgacaatgc ggccgccctt gcgctttacc gcaagctccg gttcgtagaa
     1561 aggggccgac gcgagggtta ctacgtcggg gcgggtgccg ggcccgtgga cgctgttaca
     1621 tttgcccgcg ccttgtagta gagtcgtggg cccgaggatt gcggcaaacg ccaggctcac
     1681 ctataagcaa agaagcaagg attctctgtc gttatcaacg gcatgtctgt cttgatcgaa
     1741 accttcccga gccgtatcga gcgcctttgc gccgaaaagc agctccgtct gaccgggcag
     1801 cggcgcatta tcgcgcgcgt gctctccgac tccaaggatc acccgcacgt ggaggaggtc
     1861 tatcggcgcg ctaacgccgt cgactcgcgc atttcgctct ccacggtcta ccgcacgctg
     1921 aagctgttcg ccgccaaggg tattctggag cggcacgatt ttggcgccgg ccgcggccgc
     1981 tacgaggagg cagccggcaa gcaccacgat catttgatcg atattgaaac cggcaaggtc
     2041 atagaattcc gcaacgagga aatcgagcgc ctgcaggagc gggtcgcgcg cgagctgggt
     2101 ttcaccttgg tcggacaccg cctcgagctg tttggcgtgc ctttgaaacg ggtgcgcaag
     2161 taagcgcgcc c
//
LOCUS       DASTMT010000054         3331 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_4608583, whole genome shotgun sequence.
ACCESSION   DASTMT010000054 DASTMT010000000
VERSION     DASTMT010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3331)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3331)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3331
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4608583"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1134)
                     /locus_tag="VFR00_01155"
     CDS             complement(<1..1134)
                     /locus_tag="VFR00_01155"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0157883.1"
                     /translation="MAPFQLRSFRLQWPADLLTSCAFEMETLILGWYVLVETNSVLLL
                     TLFAALQFTGTLVAPVFGVAGDRIGHRNLLCGMRVVYTILATTVMAVALAGTPNPVIV
                     LTVTALMGLVRPSDLGVRSALIADAMPTEQLVPALGISRTTSDMARVIGALTGAGLFA
                     AFGLGAAYIVVASFYALGFVLTSAIASPSRGGQPEAAIVAPRASAWSDLRAGLGYVRA
                     RPQLLAAMWLAFLVNLTAYPLSNGLLPYVAREIYLIDQTGLGYLAASFAMGALLGSMA
                     VSQRSTIRPGRAMIVATLAWYLALLVFVRLTSPFGGGVMLMLAGFAQSFCMVTLAVML
                     MRTTSAPFRGRVMGVRMLVIYGLPLGLPVAGALIDRVGFHATAT"
     gene            1718..1942
                     /locus_tag="VFR00_01160"
     CDS             1718..1942
                     /locus_tag="VFR00_01160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0157884.1"
                     /translation="MAKSLRAAVSQEASPVFRLNLWAVPAVEREWVRVGAWVLLRAWL
                     ERLSRRGTLGYYRARHRPLAGPAPGARLPQ"
     gene            2518..2682
                     /locus_tag="VFR00_01165"
     CDS             2518..2682
                     /locus_tag="VFR00_01165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0157885.1"
                     /translation="MAIERNIAGLRRVGDEGADTRLHLRQSVADAAERVPLSAPNFAL
                     ADYRDRHREK"
     gene            complement(2717..>3331)
                     /locus_tag="VFR00_01170"
     CDS             complement(2717..>3331)
                     /locus_tag="VFR00_01170"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016848511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006102 - isocitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HEU0157886.1"
                     /translation="RNYPVYLSTKNTILKVYDGRFKDIFQEVFDREFKTEFDKRKLTY
                     EHRLIDDMVAAALKWSGGYVWACKNYDGDVQSDTAAQGFGSLGLMTSVLMTPDGKVVE
                     SEAAHGTVTRHYRQHQQGKETSTNSMASIFAWSRGLAHRAKLDANGELAKFAATLEKV
                     AVATVEAGDMTKDLALLVGDQQKWLSTTAFLDKIDANLKKAMAA"
BASE COUNT          609 a         1081 c         1099 g          542 t
ORIGIN      
        1 cgtggcggtg gcatgaaaac cgacgcgatc gatcagggcg cccgccaccg gcagcccgag
       61 cggcagcccg tagatgacca gcatacgcac gcccatgacg cgaccgcgga acggcgcact
      121 ggtcgtgcgc atcagcatca ccgccagcgt gaccatgcag aagctctgcg caaaacccgc
      181 aagcatcagc atgacgccgc ccccaaacgg gctcgtcaac cggacaaaca caaggagcgc
      241 gagataccag gcaagcgtgg cgacgatcat ggcgcgaccg ggccggatgg tgctgcgttg
      301 gctgacagcc atcgagccca gcagcgcgcc catcgcaaag ctggcggcga ggtagccgag
      361 ccccgtctga tcgatcagat agatctcgcg cgctacatag ggcaacaggc cattggagag
      421 gggataggcc gtcaggttga cgagaaatgc aagccacatg gcggccagca actggggtct
      481 cgcgcgcaca tagccgaggc cggcgcgcag atcgctccag gctgaggcgc gcggcgcgac
      541 gatggcggcc tccggctgtc cgccccggga ggggctcgcg atggccgagg tcagaacgaa
      601 gcccagggca tagaagcttg ccaccacgat gtaggcggcg ccgagaccga aggccgcaaa
      661 caggccggcg cctgtcagcg cgccaatcac gcgggccatg tcggaggtcg tacgcgagat
      721 gcccagagcg ggcaccagct gctcggttgg catggcgtcc gcgatcaacg ccgagcgcac
      781 accgagatcg gatggccgca ccaatcccat cagggccgtc accgtcagga caatcacggg
      841 gttcggcgtg cccgcgagcg cgacggccat cacggtggtc gcaaggatcg tgtagaccac
      901 gcgcataccg cacaacaggt tgcggtgacc gatacggtcc cccgcaaccc cgaacaccgg
      961 cgcaaccagc gtgccggtga actgcagtgc cgcgaataac gtcagcagca gcaccgaatt
     1021 ggtttccacg agcacatacc agccgaggat gagggtctcc atctcgaagg cgcaggaggt
     1081 caacagatcg gctggccatt gcagtcggaa gctgcggagt tggaaaggcg ccaaggcctg
     1141 tgccctgaat aatgggctca cgtcacggcc aagcgataga tcaaagtccg cccctcccgc
     1201 ctcggcgccg cctcgggcgc agccgcccca tgcacaatag aggggcggag tgctgcaggg
     1261 aaaggcagcg cgcgcaaccg tgctatgtcc gcaaactccg cccgcagccc tgccgccgag
     1321 agaccatcgc tgccgtagcg ccgcggcgaa gccgatcgag gccgcggccg gcagcgctca
     1381 agcttggcgc gctgtcggct acgctcagga atgcggcgac tgcaaggggg cggctacgcg
     1441 cgcgacacat ggctcacccc tgttcttggt ggggcccgca atccaaggga aatgctaggg
     1501 agatggagcg ggttaacccc gaggcagagc ctagaaaaac ctccaattgc cacccttcgt
     1561 tccctgcgga ttgagcatat gtggccgcct cgtggcagcg gaacggccgc tggcgctcgc
     1621 cgttttagca ggcgagcaaa acctgaggcg cgagcgtgcg ggcagatcgc ccgtggctgc
     1681 gaccagcagg tgcgatgatc aagagctgga gtagaaaatg gcgaaatctc taagggctgc
     1741 cgtcagccag gaggccagcc ccgttttcag actgaacctg tgggccgtgc cggccgtcga
     1801 gcgcgagtgg gttcgcgttg gcgcctgggt cctgctgcgg gcgtggctgg agcgcctctc
     1861 ccgccgcggc acgcttggct attaccgggc acgtcaccgt cccctggcgg gtccggcgcc
     1921 cggggcgcgc ctgccgcaat agggtcggcc aggccaggat tgcccggtaa acgctcccgc
     1981 aagttcccgc catccttggc gctggcggcc gtatccgctt cgagttgggc gatctcgtcg
     2041 cgcaacaact tctgatcctg acccgccgcg ccatctgcag ctggatgctg cccacctccc
     2101 ggttgagcgc gtcgcgggcg gatccaaacg tgccgtaggt gacgacgatt cgctgctgat
     2161 cggcgcggtt ggcttcctcg cgttggcgca cgcgctcttt caagttgctc caacctggcc
     2221 aagctctgcg cgcggttcgg atccgtgcgc cgtgccactc cgcttccaat tgcaagcgct
     2281 gctgcagcgc ctccgtgtcg cttgccggcg cgtcgggctg aggcgcggca ggctacccgg
     2341 cgccgcggcg taatgaaaat agaactcgcc cgtcagcgcg gagtgatccc acggcacctg
     2401 cttgccgtgc gtcgtcgttg agcacctgtt tgcgcacctc gatcatgacc gcggtcagtt
     2461 gcgtccgggt tcccgcaggc ctgccagcaa agccgccgtg aagagcgagt tgcggccatg
     2521 gccatcgagc gcaacattgc ggggctgcgt cgagtaggcg atgaaggtgc cgacacccgt
     2581 ctgcacctgc gccagtccgt tgccgatgct gccgagcgcg ttccattgag cgcgccaaat
     2641 ttcgcgctag cggattatcg cgacaggcat cgagaaaaat gacgtttgac tttctcgggc
     2701 tcgcgcgagc gggccctcag gcggccatgg ccttcttcag gttggcatcg atcttgtcca
     2761 ggaaggctgt ggtcgagagc cacttctgct gatcgcccac cagcagggcc aggtccttgg
     2821 tcatgtcacc cgcttccacc gtggcgaccg ccaccttctc caacgttgcg gcaaatttcg
     2881 cgagctcgcc gttggcatcg agcttggcac ggtgcgccag gccgcggctc caggcgaaaa
     2941 tggaagccat cgagttggtc gaggtctcct taccctgctg gtgctggcga tagtggcgcg
     3001 tcacggtgcc gtgggcggcc tccgattcga cgaccttgcc atcgggggtc atcagcacac
     3061 tggtcatgag acccagagag ccgaagcctt gcgcggcggt gtccgattga acgtcaccat
     3121 cgtagttctt gcacgcccac acgtagccgc cagaccattt gagtgccgcg gccaccatgt
     3181 cgtcgatcag gcggtgctcg taggtgagct tgcgcttgtc gaattcggtc ttgaactcgc
     3241 gatcgaacac ctcttggaag atgtccttga agcggccgtc gtagaccttg aggatggtgt
     3301 tcttggtgga caggtagacc ggatagttgc g
//
LOCUS       DASTMT010000055         3926 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_4609320, whole genome shotgun sequence.
ACCESSION   DASTMT010000055 DASTMT010000000
VERSION     DASTMT010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3926)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3926)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3926
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4609320"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            282..1478
                     /locus_tag="VFR00_01175"
     CDS             282..1478
                     /locus_tag="VFR00_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015824401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20 aminoacylase family protein"
                     /protein_id="HEU0157887.1"
                     /translation="MPEPSGAHATLDRVRTYHSELTHVRRDIHAHPELGLNTHRTADV
                     VARLLQSWGIEVHRLVEGAGVVGVLRCGNDPRAIGLRADMDALPIHEQSGASYKSGTA
                     GVMHACGHDGHTTMLLGAARYLAETRRFSGTVNFIFQPGEEGMGGALAMLADGLLQRF
                     PSDALYGLHNRPGVPVGHFSITPGRAMAGGAFFDITVEGRGAHGAWPDAAIDPVITCC
                     HIGAALQTVVARNLSPAEMGVLSVTKIHSGDAYNVIPERASMGGTARAMKRETLGVIE
                     AGLKRVASGIAAGLGAKVEIDYRLIFAPLVNAAEPTQAIIDTAAELVGEGNVERNKPP
                     ASASEDFAFMLEKVPGAYINLGNGQTSAPVHNDRYDFNDAALPFGAALFARLVERTLA
                     TEPGPT"
     gene            complement(1566..2213)
                     /gene="eda"
                     /locus_tag="VFR00_01180"
     CDS             complement(1566..2213)
                     /gene="eda"
                     /locus_tag="VFR00_01180"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015931050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008675 -
                     2-dehydro-3-deoxy-phosphogluconate aldolase activity
                     [Evidence IEA];
                     GO_function: GO:0008700 - 4-hydroxy-2-oxoglutarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0009084 - glutamine family amino acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEU0157888.1"
                     /translation="MLPRERLAAILAKNWLIPVIAIEESSAAVPLAQALVAGGVTALE
                     VTLRTAAASEAIRQIVREVPQAIVGVGTIRSADDLRHAVDLGAQFGVSPGTTPQIYAA
                     CAAAKLPFLPGAATASEILLALEHGWETIKFFPAVPAGGIAAIKALRGPFPTLQFCPT
                     GGIDEANLGDWLSTPGVVAIGGSWLAPNDKVRARDWAGITEIARRSIARGSPRQS"
     gene            complement(2213..3166)
                     /locus_tag="VFR00_01185"
     CDS             complement(2213..3166)
                     /locus_tag="VFR00_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006019748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEU0157889.1"
                     /translation="MRPLAAIGECMLELSEHPDGRITRAFGGDTLNTAVYLARLGVPV
                     DYITALGDDAWSQEMLAAWQAEGVGTASVLRLEGRLPGLYIIQTDARGERRFSYWRDR
                     APARDLFRLPQTPSLCLGLLRYGHIYLSGISLSLYGAEGRAVLVDALTQARNAGSKIV
                     FDTNFRPRGWPDRDLAMAAYATVLGLADIVFASVEDLSLLLGSDGPNVLDRQAPGAEI
                     VLKLDYPACRITCEGSTLEIAAEGVPAVVDTTAAGDSFAAAYLSARFAGLGPATAARA
                     GHALAGAVVQHRGAIMPRAAWPRNELAAIGLKGSPHKGDIS"
     gene            3518..>3926
                     /locus_tag="VFR00_01190"
     CDS             3518..>3926
                     /locus_tag="VFR00_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0157890.1"
                     /translation="MHTWIAALLLTIAPFGLASTALAQAGDQGTELAFMPRTLDVTLL
                     NEGGKYVAIDLKHIPQGGTCRMDKDATIARMGPGASPGTTRVRYVAPQVNSGGCPFLT
                     AFDLSDEDYAVARGKFVQMKEEATKKVEELKKDL"
BASE COUNT          691 a         1296 c         1270 g          669 t
ORIGIN      
        1 gcgggtcgaa ctggcgcaaa cctgatcggc aatgtcgcgt gcggagcgcc tgcgcacttg
       61 gcattggcgc cgctgccgcg caaggcatga gggaaccatg ccgcgaccgc ccgcgtcctg
      121 cgtctttgct cccgcgccag aggacgctgt cgccagggcg gccgcgcccg ggtaaatcgc
      181 tgcggggacc atcggcgagg gcccttgctc ttccagctgt taggggcaat gtttgcttcc
      241 gccatgccct gcctcgagcc caacgcaaaa aggaagccgt tatgcccgag ccttcaggcg
      301 cccacgcgac gctcgatcgc gtacgcacct atcattcgga gctgacgcac gtccggcgcg
      361 atatccacgc tcatcctgag cttggcttaa atacgcatcg cacggccgac gtggttgctc
      421 gcctgctcca gtcctggggc attgaggtcc accgccttgt cgagggcgcc ggcgtcgtag
      481 gcgtgctgcg ctgcggcaac gacccgcgcg ccatcgggct gcgcgccgac atggacgcgc
      541 tgcctatcca cgagcagtca ggggctagct acaaaagcgg cacggcgggt gtgatgcacg
      601 cctgcggtca cgacggacac acgaccatgc tgctcggcgc tgcccgctat cttgcggaaa
      661 cgcgccgctt cagtggcacc gtcaatttca tatttcagcc aggggaagaa gggatgggtg
      721 gggcgcttgc catgctggcg gacggcctct tgcagcgttt tccctcggac gcgctctatg
      781 gtttgcacaa tcgcccgggc gtgcctgtgg gacatttctc gatcacgccg ggtagagcca
      841 tggcgggtgg ggcctttttc gacattaccg tggagggccg cggcgcccat ggagcctggc
      901 ccgacgcggc catcgatccg gtgataacct gctgtcacat cggggctgcc ctgcagaccg
      961 tcgttgctcg caacctgtcg cccgccgaga tgggcgtgct cagcgtgacc aaaattcaca
     1021 gcggcgatgc ctacaatgtc attcccgaac gggcctccat ggggggcacg gcgcgcgcga
     1081 tgaagcgcga aacgctcggg gtcattgagg cagggttgaa gcgggtagcg agcggcattg
     1141 ccgcgggcct gggtgcaaag gtcgaaatcg actaccgcct tatctttgct ccgctcgtca
     1201 acgcggccga gccgacgcag gccatcatcg atacggcggc cgagctcgtc ggggaaggga
     1261 acgttgagcg caacaagcca ccggcgtcgg cgtcagaaga tttcgctttc atgctcgaga
     1321 aggtgccagg tgcctacatc aacctcggca acggccaaac gagcgcgccc gtgcacaacg
     1381 atcgctacga cttcaatgac gcggcactcc ccttcggcgc cgccctgttt gccaggcttg
     1441 tcgaacgcac gctcgcaacc gagcccggcc caacctgaag gcgcggctcg ggctttgagc
     1501 caaccgacgt gcactgccgc acgcgagcgg ggcctgtgcg catactcgtt gcgccaattt
     1561 cggccttagc tttgccgcgg gctgccgcga gcgatgctgc ggcgggcgat ttcggttatg
     1621 cccgcccagt cgcgcgcccg caccttgtcg ttgggtgcca accaggatcc gccaatggcg
     1681 accacaccag gcgtactgag ccaatccccg agatttgctt catcgatgcc acccgtgggg
     1741 cagaactgca gcgtcgggaa cggtccccgc agcgctttga tggccgcaat accgccggcc
     1801 ggaacggcag gaaagaattt tatcgtttcc cagccatgct cgagcgcgag caggatctct
     1861 gaggcggtcg cggcaccggg caggaagggc agtttcgccg ccgcgcacgc cgcgtagatt
     1921 tggggggtgg tgccgggtga cacgccgaac tgggcgccga ggtcgaccgc gtggcgtaga
     1981 tcgtccgccg agcggatcgt tcccacgccc acaatggctt gcggcacctc gcgcacgatc
     2041 tgccgaatgg cctcgctcgc cgcggcggtg cgcaaggtca cctccagggc cgtcacgccg
     2101 cccgccacca gcgcttgcgc cagcggcacg gcggcgctcg actcctcgat ggctatgacc
     2161 gggatcagcc agttcttcgc tagaattgcg gcgagccgct cacgtggcaa catcagctga
     2221 tatccccttt gtgcgggctg cccttgaggc cgatcgccgc cagctcgttc ctcggccagg
     2281 cagcgcgggg catgattgcg ccgcgatgtt ggacaaccgc ccctgccagt gcgtgccccg
     2341 cgcgcgccgc cgttgccggg cctaagccgg caaagcgtgc gctgagatag gcggccgcaa
     2401 agctgtcccc cgccgcggtc gtgtcaacca cggccggcac gccttcggcc gcgatctcga
     2461 gcgtgcttcc ctcgcacgtg atgcggcagg ccgggtagtc cagtttcaag acgatctcgg
     2521 cgcccggagc ttgccgatcc aatacattcg gcccgtcgga tccgagcagc aaactcaaat
     2581 cctcgacgga ggcgaacacg atgtcggcca ggcccaacac cgtcgcatag gcggccattg
     2641 ccaggtcccg atccggccag ccgcgtggac gaaagttggt gtcgaacacg attttgctgc
     2701 cggcattgcg agcctgcgtc agagcgtcaa ccaacacggc ccggccctca gcgccgtaaa
     2761 gcgaaagtga gatgccggag aggtagatgt gtccatagcg aagcagcccc aggcaaaggg
     2821 atggagtttg tggcaagcgg aagaggtcgc gtgcgggggc tcgatcgcgc cagtaggaaa
     2881 accgccgctc gccacgagca tcggtctgga tgatgtagag cccgggtaat cggccctcca
     2941 ggcgcagcac cgaagccgtc ccgacgcctt cggcttgcca tgccgccagc atttcctggc
     3001 tccaggcatc atccccaagc gcggtgatgt aatcgaccgg cacgccgagg cgcgcgagat
     3061 agacggcggt attcaaggtg tcgcccccga aggcacgcgt gatgcggccg tcgggatgtt
     3121 cgctgagctc gagcatgcat tccccgatcg ccgccagagg tctcatgcca cgcttccctt
     3181 ctgccgccgg cgaggtcgct tcggggcttc gagctcaagt gtcgccaagc tcgccatggg
     3241 gtttggcccc cttatgcggg gagcgcgcgg tagaatttaa ctctgcagtc ccgctgccgg
     3301 tccccatcac cggcctgcac aggcatagga gggccgcatc ccctaagcaa gggcgcagct
     3361 ggggcgactt ccagcctcgc aaggccaggc gccccgcctg ctgcgggcac gacggcggcc
     3421 gatcggccgt tgcgcacttc gccgccccct ggccggaaca gaagcctgag catcgccgtt
     3481 atttggtcga tcgcgccccg ctcaaggagc cacttcgatg cacacttgga tagctgccct
     3541 tctcttgacc atcgcgccgt ttggccttgc cagcacagcc ctggcgcagg cgggggatca
     3601 aggcacggaa ttggcgttca tgccgcgaac cctggacgtg actttgctca acgaaggcgg
     3661 caagtatgtt gcgatcgatc tcaaacacat tccccaaggg ggaacctgcc gcatggataa
     3721 ggacgcaacc atcgccagaa tgggtcccgg cgccagtccg ggaacgacgc gcgtccgtta
     3781 tgttgcacca caggtgaact ccgggggatg cccgttccta acggcgtttg atctcagtga
     3841 tgaagattat gccgtcgcgc gcggtaagtt cgtgcaaatg aaagaggaag cgacaaaaaa
     3901 ggtcgaggag ctgaagaagg accttg
//
LOCUS       DASTMT010000056         5856 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_6250047, whole genome shotgun sequence.
ACCESSION   DASTMT010000056 DASTMT010000000
VERSION     DASTMT010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 5856)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5856)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5856
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6250047"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(151..999)
                     /locus_tag="VFR00_01195"
     CDS             complement(151..999)
                     /locus_tag="VFR00_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012116074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEU0157891.1"
                     /translation="MKLVRFGAPGAEKPGLIDAEGGVRDLSAHVKDITGETLAPASLD
                     KLRKLDPRSLPAAPAGVRLGPPVGRVQNFIAVGLNYIDHAKETGAEIPKEPILFNKLP
                     NCIVGPNDDVMVPKGSLKLDYEIEIAFVIGQRARYVSEKDALSYVAGYTICNDISERH
                     FQAERGGQWMKGKCAETFGPLGPWLVTRDEIANVQTLGMALDVNGQRRQTGNTETMIF
                     GIQHCMHYISQFMVLEPGDVVTTGTPPGVALGMKPPVWLKAGDVMTLKIEGLGEQRTK
                     VVAFQA"
     gene            complement(1552..4875)
                     /locus_tag="VFR00_01200"
     CDS             complement(1552..4875)
                     /locus_tag="VFR00_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015596280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like domain-containing protein"
                     /protein_id="HEU0157892.1"
                     /translation="MEVGPAPDLDRLSAAFEHSITWEDDIWRADPVDVEIVHAHARRK
                     FNDLLESVTGAQGALGQARILLFHGQSGAGKTHLIRALRTSAHRSGKAYVGYAQMTPD
                     VANYADYYLRRLVNSLEKPYDPDTGGETALQRLTRRLTEAAESLDTTALDKLRDAKLS
                     DAQLAKIVLTLADQVVAAPKFAGQPLDINIVRALLYLQRADPRIDQRVRQYLFGRELT
                     DLSAEAVAVLDPNRGEGRAFEIIESLGRLMWIVDGAALVISIDQVEDLRFFEDYHERF
                     QKAARDLIQIANRVPTSIVLLSCLGDFYGKVREVLAQSYIDRIEKAGPVSLKEARTAE
                     EAQLIIAKRIQADASRSDIDPSLFFGPQFFDEFSGLSTRRILELAQARVREQVAEADP
                     EPEEAKGGFISTLAAALGFGGRAEADGADAHALVEFHERWDRFTAQSEAEIPSDEHEL
                     LTVLTNALTLARAEWGGALDVVVKRLDLGADLPALDLSLKHEGGPSTEARVFLCNRPT
                     QGGGLKRQLDKLLATMDGATTFLLRASDFPANKKNQTGQAFRKFRELGGRTVLVPIPD
                     WERMMMVCEFHAQHRRSSGFAQWFERTKLLADILSLAQLLRLELFGRSINARVPAASE
                     LAAAEAAPEPPLGAGFPAAAADWTGWGPPTWGAAVPAGKEADLPLSVLLDETGDAAAS
                     ILAGRALNSSKSITLNRNVLKRHAAVLGGSGSGKTTLALCIIEQLLLRGTPVVLIDRK
                     GDLCSYANPDVWRAHEGEFSERRGEREKLADSIDVAVYTPGRASGRPISITLLPNGIN
                     ELPDQEQQLLANMSAAALGDMLHFKNSATHQKQSGTLSVALRILGSRFSKEVTLADLI
                     YLLEDEDPELTDLTQRMDPSGRIRRDLVAQLDSLRHRNAALFEAGGEPLRMDSLLGMG
                     RYGRTDGRTRLSIIYTGFLGDNENILFWVSQFLSEALRFCQRNPNDELQAVVMFDEAD
                     LYIPANAKPATAEPLQSLLKRARSAGLGIMLATQSPGDLDYKSRDQITSWFIGRVRED
                     TALRKLRAAFQSESGLDPGAVLPGQTVGEFHLVQEGLVRPMRAQKSLIVAEQVPFDRI
                     EQLARETKGQDEKQLKLFA"
     gene            complement(4921..>5856)
                     /locus_tag="VFR00_01205"
     CDS             complement(4921..>5856)
                     /locus_tag="VFR00_01205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0157893.1"
                     /translation="ESLLGEGAVRENAGHLRVTTEGAEAARRFVGGRGALPRAWSELR
                     DVRLVAKALGLERLAGKDLKLLARPDGLRAGVVQAAYKLKIKGVATPSRLRAALAALA
                     LERAFGNQLGATLSGKSALPAKAGRLLAGQLARNPRDFGTDRRLIAALAGEHLGVKPA
                     DIEALRRGVICRFLADALAEAGEGARAPRGPARLEAVGPRVAPAPLASSAGARPDLPL
                     FVHEVRREAGRLAEGWIGNRKAYISHVWRALAQARTEWGLSEIEFKCMLLEAHRAGQL
                     ALAHADLKDHKSLKDVQDSAVVYKNAVFHFVRADG"
BASE COUNT         1044 a         1924 c         1923 g          965 t
ORIGIN      
        1 attaagcgca tcgacaaatc gcgcccggaa gcctggaaga aggtgaccga gatgatcgag
       61 gcgcgtcgga gcgacgcgcc aacccacacc atcggcgagc tcgatcccgc caacaagcag
      121 ccgatttgag gctgcaagcg cggccctacc tcacgcctgg aaggcgacga ccttcgtgcg
      181 ctgctcgccc aagccctcga tcttgagggt catcacgtcg ccggccttga gccacaccgg
      241 cggcttcatg ccgagcgcaa ctccgggcgg agttcccgtg gtgacgacgt cgccgggttc
      301 gaggaccatg aactgcgaaa tataatgcat gcagtgctgg atcccgaaaa tcatggtttc
      361 ggtgttgccg gtctgacggc gctggccgtt gacgtcgagg gccatgccga gcgtctgcac
      421 attggcgatc tcatcgcgcg tcaccagcca agggccgagc ggcccaaaag tctccgcgca
      481 tttgcccttc atccattggc cgccgcgctc ggcctggaag tgacgctccg agatgtcgtt
      541 gcagatggtg tagccggcga cataggagag cgcgtctttt tccgagacat agcgcgcgcg
      601 ctgtccaatg acgaaggcga tctcgatttc atagtcaagc ttgagcgagc ctttgggaac
      661 catcacatcg tcattgggtc cgacgatgca attgggcagc ttgttgaaca ggatcggctc
      721 cttcggaatt tccgcgccgg tctccttggc gtggtcgatg tagttgaggc cgacggcaat
      781 gaaattctgc acccggccga ccggcggccc gagccggacg cctgccggtg cggccggcag
      841 cgagcggggg tcgagcttgc gcagtttgtc gaggctcgcc ggcgcgagcg tctccccagt
      901 gatatccttg acgtgcgccg agaggtcgcg cacgccgccc tcagcgtcga tcaagccagg
      961 cttctcggcg ccgggtgcgc caaaacgtac gagcttcatg gaaggtcctt tctcacctgt
     1021 ggggcgcaac aacaccccaa gccgtgccga ccgcaatcgg cacctgcccg ccccctctcg
     1081 tcaagagcca agggccgcgc ccggcgcaca ggcggcccgg gcgtcaacgg gggcgccgcg
     1141 tcagggtttt gcacaggcct gggaattaac ggaatgatgc ggcacggcca cgtctgcgca
     1201 ccaccggcgc gcccttgcgg tcacacagcc gttccgcccg tcaagctcag aagaggttga
     1261 cgaccagata gatcccaacg ccgaggaaag gagccatggc acacgccaat gccatgatta
     1321 gaaaggactt gtcgatcacc acacgacccc gcacgacaca acacgcaact aagccggctc
     1381 atccccctcg cccccagctc agcggagcgg ccccctcaga ccgatccgac ctcagaatag
     1441 tgcaaagaag ccagaaatgc acgccttttg cagcgcttag gagggcaccc ggcttttgca
     1501 ttgcaacacg ggcgggcgag tgccggcccg cctccggcac gggtccgagc ctcaggcaaa
     1561 cagcttcagt tgcttttcgt cttggccctt ggtctcccgg gccagctgct cgatgcggtc
     1621 gaagggcacc tgctcggcga caatcaagga cttctgggcc cgcatcgggc gcacaagccc
     1681 ctcctgcacc agatgaaatt caccgaccgt ctggccgggc agcaccgccc ccggatcgag
     1741 gcccgactcg ctttggaagg cggctcggag tttgcgcaag gcggtatcct cgcgtacgcg
     1801 gccgatgaac caggacgtga tctgatcgcg cgacttgtag tcgaggtcgc ccggcgactg
     1861 ggtcgccagc ataatgccga gcccggccga gcgggcgcgc ttcagcaagc tttgcagggg
     1921 ctcggcggtg gcaggcttgg cgttggcggg aatgtagagg tcggcctcgt cgaacatcac
     1981 cacggcctgc agctcgtcat tgggattgcg ctggcaaaag cgcagcgcct cggacaggaa
     2041 ctgggagacc cagaacagga tgttttcgtt atcgcccaaa aagccggtgt aaatgatgga
     2101 caggcgcgtg cgcccgtcgg tgcggccgta gcggcccatg ccgagaaggg agtccatgcg
     2161 caacggttcg ccgccggctt cgaacagggc ggcgttgcga tggcgcaaac tatcgagctg
     2221 ggccacgaga tcgcggcgga tgcgccctga cggatccatg cgctgtgtca ggtcggtaag
     2281 ttcggggtcc tcatcctcca agaggtaaat gagatcagca agggtgacct ccttgctgaa
     2341 gcggctgcca agaatgcgca gcgccaccga aagcgtgccc gactgctttt gatgcgtggc
     2401 ggagttcttg aagtgcagca tatcgccgag ggcggcggcc gacatgttgg ccaacaattg
     2461 ctgctcctgg tcgggcagct cgttgatgcc gttgggaagc agcgtgatcg agatcggccg
     2521 gccggaggcg cggccgggtg tgtagacggc cacatcgatg gagtcggcga gcttctcgcg
     2581 ctcgccgcgg cgctcggaaa attccccctc gtgcgcgcgc cacacgtccg ggttggcgta
     2641 ggaacagagg tcacccttgc ggtcgatcag caccaccggg gtcccacgca agagcagctg
     2701 ttcgatgatg cacagcgcca gcgtggtctt gccggagccc gatccgccga gcacggccgc
     2761 atgccgcttc aagacgtttc ggttgagcgt gatgctcttg ctggaattaa gcgcgcggcc
     2821 agccagaatg ctggccgcgg cgtccccggt ttcatccagc agcaccgaga gcggcaagtc
     2881 cgcttccttg ccggccggta cggcggcgcc ccaggtcggg gggccccagc ccgtccagtc
     2941 ggcggccgcg gccgggaagc ctgcgcccag cggtggctcg ggcgcggcct cggcagccgc
     3001 gagctcgctc gcggccggta ccctggcatt gatgctgcgg ccgaacagct cgagccgcag
     3061 cagctgggcc agcgaaagga tgtcggccag cagcttggtg cgttcgaacc actgggcaaa
     3121 accggagctg cgacgatgct gggcatggaa ctcgcacacc atcatcatgc gctcccaatc
     3181 cgggatcggc accaggaccg tgcgcccacc caactcgcgg aatttgcgga aggcttgtcc
     3241 ggtctggttc ttcttgttgg ccggaaagtc gctggcgcgc agaagaaagg tagttgcccc
     3301 atccattgtg gcgaggagct tatcgagctg tcgcttgagg cccccgccct gggttgggcg
     3361 attgcacagg aagacccgcg cctccgtgct cggcccacct tcgtgcttca ggctcaaatc
     3421 cagcgccggc agatcggcgc cgaggtccag gcgcttgacg acgacgtcga gggcaccgcc
     3481 ccactcggcc cgcgccagcg tcagcgcgtt tgtcagcacc gtcagcaatt catgttcgtc
     3541 ggagggaatt tcggcctccg actgggcggt aaagcggtcc cagcgctcgt ggaattcgac
     3601 gagggcatgc gcgtcagcgc catcggcctc agcgcggccg ccaaagccaa gcgcggcggc
     3661 gagcgtcgaa atgaagccgc ccttggcctc ctcgggctcc gggtcggcct cggccacctg
     3721 ctcgcggacg cgcgcctggg cgagttccag aatgcgccgc gtcgacaagc cgctgaactc
     3781 atcaaagaat tgcggaccga aaaacagcga aggatcgatg tcactgcggc tggcatcggc
     3841 ctggatgcgc ttggcgatga tgagctgtgc ctcctccgcc gtgcgcgcct ccttcaggga
     3901 tacgggaccg gccttctcga tgcgatcgat ataggattgc gccagcacct cgcgcacctt
     3961 gccatagaaa tcgccaaggc aggagagcag cacgatcgac gttggcacgc gattggcgat
     4021 ttgaatgaga tcgcgggcgg ccttctggaa gcgctcatgg tagtcttcga aaaagcgcag
     4081 gtcctccacc tgatcgatgg agatgacgag cgccgcgccg tccacgatcc acatcaagcg
     4141 gccgagcgac tcgataattt cgaaggcgcg cccctccccc cgattgggat cgagtaccgc
     4201 cacggcctcg gcggacagat ccgtcagctc gcggccaaac aggtattgcc gcacgcgttg
     4261 gtcaatgcgt gggtcggccc gctgcagata gagcagtgcg cgcacgatgt tgatgtcgag
     4321 gggttgccca gcaaacttcg gcgcggcgac aacttgatca gccaacgtca acacgatttt
     4381 ggccagctgc gcgtcgctga gcttggcatc gcgcagcttg tcgagggccg tggtgtcgag
     4441 ggactcggca gcctccgtca gccttcgggt gaggcgttgc agggcggtct caccgcccgt
     4501 atccggatcg tagggctttt ccagcgaatt aacgaggcgg cgcaggtaat agtcggcgta
     4561 gttggcgaca tccggtgtca tctgggcgta gcccacatag gctttgcccg agcgatgcgc
     4621 cgaggtgcgc agcgcgcgga taagatgcgt tttgccggcg cccgactggc cgtggaacaa
     4681 caggatgcgc gcctggccca gcgcgccctg cgcgcccgtt accgactcga gcagatcgtt
     4741 gaatttgcgc cgggcgtgag cgtgcacgat ctcgacatcg accggatcgg cgcgccaaat
     4801 atcgtcctcc caggtgatgg agtgctcgaa ggccgccgac aggcggtcca gatccggcgc
     4861 ggggccaacc tccatgcgct tgtcacctca acttggcgtt ctctagcagc gaccgcggcc
     4921 tcagccgtcg gcccgcacga aatgaaacac ggcgttcttg tagacgacgg cggagtcctg
     4981 cacgtccttg aggctcttgt gatccttgag gtcggcatgg gcgagcgcca gctgcccggc
     5041 gcgatgcgcc tccaacagca tgcacttgaa ctcgatctcg gaaaggcccc actccgtgcg
     5101 cgcctgcgcg agcgcccgcc agacatggga gatgtaggcc ttgcggttgc cgatccaacc
     5161 ctctgccagt cgcccggcct cgcggcgcac ctcgtgcacg aagagaggca ggtcgggacg
     5221 cgcgccggcg ctgctggcaa gcggcgccgg cgcaacacgt gggccaacgg cctcaaggcg
     5281 cgcgggccca cgcggcgcac gagcgccctc accggcttcg gccagggcgt cggccaagaa
     5341 ccggcaaatg acgccgcgcc ggagcgcctc gatgtcggcg ggtttgacgc cgagatgctc
     5401 gcccgcaagc gcggcgatca gccgtcgatc ggtgccaaaa tcacgcgggt tgcgggcaag
     5461 ctgcccggcc aacaaacgcc cagcctttgc cggcagcgcg ctcttgcccg agagcgtggc
     5521 gcccagctgg ttgccgaagg cgcgctccag ggcgagggcg gcgagggcag cgcgcagccg
     5581 tgacggcgtg gcgacgccct tgatcttgag tttataggcc gcctggacaa cccctgcgcg
     5641 taagccatcg ggtcgggcca agagcttgag gtccttgccc gccaaccgct caagccccag
     5701 cgccttggcg acaaggcgca catcgcgcag ctcgctccag gcgcgaggca gcgcgcccct
     5761 gccgcccacg aagcgccgcg ccgcctctgc gccctcggtg gtcacccgca ggtgcccagc
     5821 attctcgcgg acagcgccct ccccgagcag gctttc
//
LOCUS       DASTMT010000057         1572 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_2653563, whole genome shotgun sequence.
ACCESSION   DASTMT010000057 DASTMT010000000
VERSION     DASTMT010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 1572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1572)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1572
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2653563"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..892
                     /locus_tag="VFR00_01210"
     CDS             <1..892
                     /locus_tag="VFR00_01210"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HWE histidine kinase domain-containing protein"
                     /protein_id="HEU0157894.1"
                     /translation="PSVKELLGHSPEEVQADGGIWFDKIHELDRPLVEKMLREAPLAE
                     QEPVEYRIVCEGTQRWVRDKAFALPAETDDGRRVAGLARDVTEAKRAEQQRELLIGEL
                     NHRVKNTLALVQALAAHTRHAAVSPSDFEQAFTSRLHALGHAHDLLTQTHWKGTSLHQ
                     VIETALAPFSVYGSSQTRVEVSGPQVWVNSNTAVTLTLAFHELATNAAKYGALSTNKG
                     HVKIFWQAQPAGPPQEILLQWRESGGPPVSAPSRKGFGTMLIEKVLAYEAEGEAKLEF
                     LPTGVAFVFRLPLSSNVQLA"
     gene            1028..>1572
                     /locus_tag="VFR00_01215"
     CDS             1028..>1572
                     /locus_tag="VFR00_01215"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU0157895.1"
                     /translation="MAKKVFDQIVIGVGGMGSAAVCALARRGQRVLGLEQFGVPHELG
                     SSAASTRIFRFAYFEDPSYVPLMRLAFDRWQMLQRDYGQAFVTTTGGLDIGLPSGRVV
                     AGARFACQTHGLQHEVLRANEVSRRFPAWQLPAEFEAVWQPEAGFLLADRAIEAHVTV
                     ARRLGAEVNENESVRHWRVIG"
BASE COUNT          281 a          506 c          527 g          258 t
ORIGIN      
        1 cccatcggtc aaggagctgc tgggccactc gcccgaggaa gtgcaagcgg acgggggaat
       61 ctggttcgac aagatccacg agctggatcg cccgttggtc gagaagatgc tgcgtgaggc
      121 gccgctcgcg gagcaggagc cggtggaata ccgcatcgtg tgtgagggaa cgcaaagatg
      181 ggtacgagac aaggccttcg ccctgcccgc cgaaaccgac gatggccgcc gcgtggccgg
      241 cctcgcccgc gatgtcaccg aggcgaagcg ggccgagcaa caacgtgaac tgctgatcgg
      301 tgaactcaat caccgcgtca agaacaccct ggcgctggtg caggcgctgg cggcgcatac
      361 ccgccatgcc gccgtttccc ccagcgattt cgagcaagcc ttcacctcgc gcctgcatgc
      421 gctgggtcac gcccacgatc tcttgaccca gacgcactgg aagggcacgt cgctgcacca
      481 ggtgatcgaa acagcactcg ccccgttctc ggtttacgga agctcgcaaa cccgcgtcga
      541 ggtgtcggga cctcaggttt gggtcaactc caacacggcg gtgaccctga cattggcgtt
      601 tcacgagctc gccaccaatg cggcaaagta cggggcgctg tcgaccaaca aggggcacgt
      661 aaagattttt tggcaggctc aacccgctgg gccgccgcaa gaaatcctgc tgcagtggcg
      721 cgagagcggc ggcccgcccg tgtcggcccc ctcgcgcaag ggcttcggca ccatgctgat
      781 cgagaaggtc ttggcctacg aagcggaggg cgaggccaag ctcgagtttc taccgaccgg
      841 ggtcgccttc gtctttcgtc tccccttgtc ctcgaacgtc cagctggcct gacggcgcac
      901 ggcccgccgc aggccaaaac aggcacggcc cctggcgccg cccgagctgc gcccttcaag
      961 cccgcagcaa tcccggcacg gcgggttgcg agttgactga aacggcaagg cgacggacaa
     1021 aggtggcatg gcaaaaaagg tcttcgatca gatcgtcatc ggcgtcggcg gcatgggttc
     1081 ggcggccgtc tgcgcgctag cgcggcgcgg ccagcgcgtc ctgggattgg agcagttcgg
     1141 cgtgccgcac gaactcggat cctcggcggc ctcaacgcgc atctttcgct ttgcctactt
     1201 cgaagacccg agctacgtgc cactgatgcg gctggccttt gatcgatggc agatgctgca
     1261 gcgtgattac gggcaggcct tcgtgacgac gaccggtggg ctcgatatcg gtttgccgtc
     1321 gggacgcgtc gttgcgggcg caaggtttgc ctgtcaaacg cacgggcttc agcacgaggt
     1381 gctgcgcgcg aacgaggtca gccgccgctt tccagcctgg caactgccgg ccgagttcga
     1441 ggccgtgtgg cagcccgagg ccggctttct gctggccgac cgcgccatcg aggcccacgt
     1501 gacggtggcg cgccgcctcg gcgccgaggt caatgagaac gaaagcgtgc ggcattggag
     1561 ggtgatcggc ga
//
LOCUS       DASTMT010000058         2122 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_5921659, whole genome shotgun sequence.
ACCESSION   DASTMT010000058 DASTMT010000000
VERSION     DASTMT010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2122)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2122)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2122
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5921659"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..829)
                     /locus_tag="VFR00_01220"
     CDS             complement(<1..829)
                     /locus_tag="VFR00_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012332965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HEU0157896.1"
                     /translation="MPAKTSTDPAQMSASELLALYRRKALSPLEAVEATLARIARFNP
                     LVNAYCHVDSESALAAARAAEARWLTGSPQGLLDGVPFGVKDNIAVAGMPLRFGSRLT
                     ASGAQAFDAPAAARLKEQGAVPLGKTAMPEFGWKATSDSPLTGITRNPWNTRMTTGGS
                     SAGAVAAVTLGLGALQLGTDGGGSIRIPAGFTGCYGLKPTRARVPAFPASALGTLAHH
                     GPLTRTVTDAALMLTIIAQPDARDAYAWIDAAPDYCGGLEDGVAGLRVAFSPSLGRRV
                     "
     gene            complement(993..1856)
                     /locus_tag="VFR00_01225"
     CDS             complement(993..1856)
                     /locus_tag="VFR00_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016846141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HEU0157897.1"
                     /translation="MRDFSADLAHLAINTATLRKQLALPAILEACARRGIRRVCPWRD
                     QVASAGLASVARHLKDTGLGLSGYCRGGFFPAADATGLRAALDDNRRAVDEAKTLGAP
                     CLVLVVGALPGALQGRPAHNDIALARTQVVDGIAATLAYAREVDMPLAIEPLHPMQAA
                     DRACINTIEHALDICDALDASQTGALGVAIDVYHTWWDPKLESQIARAGRERLLAYHV
                     CDWLLPTRDLLNDRGMMGDGIVELQKIRGFVEAAGYAGPAEVEIFSERWWATPADTVL
                     DTCVARYHTVV"
     gene            complement(1856..>2122)
                     /locus_tag="VFR00_01230"
     CDS             complement(1856..>2122)
                     /locus_tag="VFR00_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008132438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF993 family protein"
                     /protein_id="HEU0157898.1"
                     /translation="FHDILAPTVPLSRHIFRAPTRFYKTGVVFMAYLNGLQDHFVMVG
                     GQQSARSLPHLAELFRLADRAGLLRDPDLARRRMVRILAVHGIA"
BASE COUNT          361 a          678 c          777 g          306 t
ORIGIN      
        1 gaacccggcg gccaaggctg ggggaaaagg ccacgcgtag gccagccacg ccgtcctcga
       61 ggccgccgca ataatcgggt gccgcatcga tccaagcata ggcgtctcgc gcatcgggct
      121 gggcaatgat ggtcagcatc agcgccgcgt cggtgaccgt gcgcgtcagc ggcccgtggt
      181 gcgccagggt gccgagcgcg gaagcgggaa acgccgggac ccgcgcgcgc gtgggcttta
      241 ggccatagca gcccgtgaag ccggcgggaa tgcggatcga gccaccgccg tcggtgccga
      301 gctgcaaggc gccgagaccc aacgtcacgg cggccacggc accggcggag gagccgcccg
      361 tggtcatgcg tgtgttccag ggattgcgcg taatcccggt gagtgggctg tcgctggtcg
      421 ccttccagcc aaattcgggc atggccgtct tacccaacgg cacggcgccc tgttccttga
      481 gccgggccgc ggccggagca tcaaaggcct gcgcaccgga cgccgtcagg cgcgaaccaa
      541 agcgcaaggg catgccggca accgcgatgt tgtccttcac cccgaagggc acgccgtcga
      601 ggaggccttg ggggctgccg gtcagccagc gcgcttccgc agcgcgggcc gctgccaagg
      661 cgctttccga atcgacgtgg cagtaggcat tgacgagggg attgaagcgc gcgatgcgcg
      721 ccagcgtcgc ctccaccgct tcgagggggg aaagcgcctt gcggcggtaa agagcgagga
      781 gctcgctcgc cgacatctgg gcagggtcgg tgctcgtttt ggctggcata ttggcgcacg
      841 ctcagggtcg ttgtggcggg caggagaggt gccccagcgt tgtcgatagc gcaaaagcct
      901 cggcaaacaa atggccagcg cgcgtctggg cccctgggtg gcagagccgc cggatgtcgg
      961 cgccgctgcc ctggggcgag cggcctggca ggtcacacca ccgtgtggta gcgcgccacg
     1021 cacgtgtcca ggacggtatc ggcgggcgtg gcccaccaac gctcggagaa gatctcgact
     1081 tcggcggggc cggcgtagcc cgccgcctcg acgaagccgc ggattttctg caactcgacg
     1141 ataccgtcgc ccatcatgcc gcggtcattg agaagatcgc gcgtcggcag cagccaatcg
     1201 cagacgtgat aggccagcag ccgttcgcgg ccggcacgcg cgatctggct ttcgagcttc
     1261 gggtcccacc aggtgtgata gacgtcaatg gcgacaccaa gcgcgccggt ttggctggca
     1321 tcgagcgcgt cgcagatgtc gagcgcgtgt tcgatagtat tgatgcaggc acggtcggcg
     1381 gcctgcatgg gatgcagcgg ctcgatggcg agcggcatgt ccacctcgcg cgcgtaggca
     1441 agcgtggcgg caatgccatc gacgacctgc gtccgcgcca gggcgatatc gttgtgcgcc
     1501 ggtcggccct gcaaggcgcc cggcaaggcg cccacgacca acaccaggca gggtgcgccc
     1561 agggttttgg cctcgtcgac cgccctgcgg ttgtcgtcca gggcggccct gagcccggtt
     1621 gcatcagcgg cgggaaagaa gccgccgcgg cagtaacctg acaggccaag gccagtgtct
     1681 ttgaggtgtc gcgccacgct ggcgaggcca gcgctggcaa cctggtcgcg ccaagggcag
     1741 acgcggcgga tgccgcgccg ggcacaggct tcgaggattg ccggtagggc gagctgcttg
     1801 cgcaaggtcg ctgtattgat cgcaagatgc gccaggtccg cggagaaatc gcgcatcagg
     1861 caatcccatg cacggccagg atccgcacca tgcggcggcg cgccaagtcg gggtcccgca
     1921 acaggccggc ccgatcggcc agacggaaca gctcggccag gtgcggcagc gagcgggcgc
     1981 tctgctggcc gccgaccatc acgaagtggt cctgcaggcc gttgagatag gccatgaaca
     2041 cgacgccggt cttgtagaaa cgggtcggcg ccctgaagat gtggcgcgac agcggcacgg
     2101 tcggggcgag gatatcgtgg aa
//
LOCUS       DASTMT010000059         1682 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_686764, whole genome shotgun sequence.
ACCESSION   DASTMT010000059 DASTMT010000000
VERSION     DASTMT010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 1682)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1682)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1682
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_686764"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..886
                     /locus_tag="VFR00_01235"
     CDS             <1..886
                     /locus_tag="VFR00_01235"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002713168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEU0157899.1"
                     /translation="LATLNPGDEVVIPAPCWVSYADIVLLGGGKPVFVPCRLEDGYKL
                     QPQDFERAITPKTKWFVFNAPCNPTGAAYSRAELKALTDVLMLPKAKHVWVLTDDMYE
                     HLIYDDAKFFTPAQVEPGLYERTLTLNGLSKTYCMTGWRLGYGAGPEPLIKAMCKLQS
                     QSTSNPSSITQWAAVEALNGPQDFIARNNQAFRERRDLVVSMLNQAKGLKCPKPQGAF
                     YVYPSCEGLIGKTSGKGTKLATDQDVVLAMLEEEDVACVHGSAFAMSPFFRISYATSI
                     DVLEEACKRIQRFCGNAH"
     gene            complement(1015..>1682)
                     /locus_tag="VFR00_01240"
     CDS             complement(1015..>1682)
                     /locus_tag="VFR00_01240"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU0157900.1"
                     /translation="ICAAVAASLGGAHNFYACDVTDAEGLEAVAARVAAELGPTDVLV
                     TSAGLIPNSEGIMDMDMAAHDRMWQVNYNGTVHACRSFARQMIPRQRGSIVTLGSINS
                     RMPLPIPAYNMGKAAIERLTQLLAVELGRHGIRVNSVGPTYVMTPPLRAKVASGQRDL
                     NKIMAVHALKQLPEPKDIANAIAFLCSAEAATITGILLPVDAGWLAGVSYRTYAGGVP
                     WQE"
BASE COUNT          290 a          547 c          546 g          299 t
ORIGIN      
        1 gcttgccacg ctcaatccgg gcgatgaggt ggtgatcccg gcgccctgct gggtgtctta
       61 tgccgacatc gtgctgttgg gcggcggcaa gccggtgttt gtcccctgtc ggctcgagga
      121 cggctacaag ctgcagccgc aggacttcga gcgcgccatc acgccgaaga ccaagtggtt
      181 cgtgttcaat gcaccgtgca atccgacggg agcagcctat tccagggcgg agctcaaagc
      241 cttgaccgac gtgctgatgc tgcccaaggc caagcacgtc tgggtgctga ccgatgatat
      301 gtacgagcac ctgatctatg acgatgccaa gttcttcacg ccggcgcagg tcgagccagg
      361 tctttatgag cgcacgttga cgctcaatgg cctgtccaag acctattgca tgaccggttg
      421 gcggcttggc tacggcgccg gccccgagcc ccttatcaag gccatgtgca agctgcagtc
      481 gcaatcgacg tccaatccgt cctcgatcac acagtgggcc gccgtcgagg ccctcaatgg
      541 cccgcaggat ttcattgcgc gcaacaatca ggccttccgg gagcgacgcg atctcgtggt
      601 ctccatgctc aatcaagcta agggcctaaa gtgcccgaag ccgcagggcg ccttttacgt
      661 ctatccgtcc tgcgagggac tgatcggcaa gacttcgggc aagggcacca agctcgccac
      721 cgaccaggac gtcgtgctgg ccatgctgga ggaggaggac gttgcctgcg tgcacggttc
      781 ggcctttgcc atgagcccgt tcttccgcat ttcctatgcc acctcgatcg atgtgctgga
      841 ggaagcctgc aagcgcattc agcgcttttg cggcaacgcg cactaggacg tcgcggcggc
      901 gcagcgccgc gatttatgcc ccagacgagg tgcggcctca cgccagcgcc gcacccccgt
      961 cgccgtcagg ccggcacctg ccgcgaaaag cgggccaagc ggggagaggc cggctcactc
     1021 ttgccacggc accccaccgg cgtaggtgcg atagctgacg ccggccagcc agccagcatc
     1081 gacaggcagc aggatgcccg tgatggtggc cgcctcggcc gagcacaaaa aggcgatggc
     1141 gttggcgatg tccttgggct cgggcagctg cttgagcgca tgcacggcca tgatcttatt
     1201 caagtcgcgt tgtcccgatg cgaccttggc gcgcagcggc ggcgtcatca cgtaagtcgg
     1261 ccccacgctg ttgacacgaa tgccgtggcg gccgagctcg acggccaaga gctgcgtcag
     1321 ccgctcgatg gcggccttgc ccatgttgta ggccgggatc ggcagcggca tgcggctgtt
     1381 gatcgagccg agggtgacga tgctgccgcg ctgccgcggg atcatctggc gcgcgaagga
     1441 gcggcaggcg tgcaccgtgc cgttgtagtt gacctgccac atgcggtcgt gcgccgccat
     1501 gtccatgtcc atgatgcctt cgctattggg aatgagcccg gccgaggtca cgagcacgtc
     1561 ggtcgggccc agctcggctg ccaccctggc cgccacggcc tccagcccct cggcgtcggt
     1621 gacatcgcag gcataaaagt tgtgcgcgcc gccgagcgag gctgccaccg ccgcacagat
     1681 cg
//
LOCUS       DASTMT010000060         1723 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_5922662, whole genome shotgun sequence.
ACCESSION   DASTMT010000060 DASTMT010000000
VERSION     DASTMT010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 1723)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1723)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1723
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5922662"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            2..1693
                     /locus_tag="VFR00_01245"
     CDS             2..1693
                     /locus_tag="VFR00_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006205346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU0157901.1"
                     /translation="MVIAPSIIDSRRHQMFPTLQPAEIARLRRFGQLQAFAKGEVLAR
                     VGEVGRGLSIILSGSVEISQRHQSAPSTPIVTHAAGALMGELAQLAGRPALVDARALE
                     PVEALIIPPERVRAMFVAEAEVGERIMRALILRRVGLLETGAGGPVIIGHSGDGDVLR
                     LEGFLARNGHPRQRLDPETDAEARALIERFHVDPGQLPIVLCPNGELLRNPGETELAR
                     CIGMVGPIDPDRIYDVAIVGAGPAGLATAVYAASEGLSVLVLDCRAFGGQAGASARIE
                     NYLGFPTGITGMALMARAYNQAQKFGAEMAIPDEAVALTGGVAESGAFQLRLFNAERA
                     RARSIVIASGARYRRLDVPRLEDFESTGVHYWASPLEAKLVAGQEIALVGAGNSAGQA
                     TVYLASQAAKVWLLVRGSSLEASMSRYLVDRIRGLENVTLVCQAEVSGLAGANGMLET
                     VRWRLASGAVIERQIRHLFLFIGAEPNSHWLAGALKLDAKGFILTGREAGGASRHLLE
                     TSRDGVFAIGDVRAGSVKRVAAAVGEGAQVVAMLHAYLAASTGESQPGSHTRPSP"
BASE COUNT          262 a          575 c          586 g          300 t
ORIGIN      
        1 catggtcatt gccccgtcga tcatcgacag ccgccggcac cagatgtttc cgaccctgca
       61 gcccgccgaa attgcgcgct tgcgccgttt cggccagctg caggcgtttg ccaaaggcga
      121 ggtgttggcg cgggtcggtg aggtcggccg cggcctcagc atcattctgt cgggcagcgt
      181 cgaaatcagc caacgtcatc aatccgcgcc aagcacgccg atcgtcacgc acgccgccgg
      241 ggcgctgatg ggcgaactgg cgcagcttgc cggccgcccg gccctcgtcg atgcgcgcgc
      301 gctagagccc gtcgaggcgc tgatcatccc gccggaacgc gtgcgggcca tgttcgtggc
      361 ggaagccgag gttggcgagc gcatcatgcg cgccttgatc ctgcgccggg tcggcttgct
      421 ggagaccgga gcgggcgggc cggtgatcat cggtcacagc ggcgacggcg acgtgcttcg
      481 gctcgagggc tttctggcgc gtaacggcca tccccgccaa cgcctcgacc cggaaaccga
      541 cgccgaggcc agggcgctga ttgaacgctt ccatgtcgat ccgggccagt tgcccatcgt
      601 gctgtgcccg aatggtgagc tgctacgcaa tcccggggag acggagctcg cccgctgtat
      661 tggcatggtc gggccgatcg atcccgaccg catctacgac gttgccatcg tcggcgccgg
      721 accggcaggg ctcgccaccg ctgtctatgc cgcctccgag gggctttccg ttttggtctt
      781 ggattgccgc gccttcggcg gtcaggccgg cgcctcggcg cggattgaaa actatcttgg
      841 ctttcccacc ggcatcaccg gcatggcgct gatggcgcgc gcctacaatc aagcccagaa
      901 attcggggcc gaaatggcca ttcccgacga ggccgttgcg ctaactggcg gggtggcgga
      961 gagcggcgcg tttcaattgc gtcttttcaa tgcagagcgg gcgcgcgccc gctcgatcgt
     1021 cattgccagt ggcgcgcgct atcggcgcct cgacgtgccc cggctcgaag acttcgaatc
     1081 aaccggcgtg cactattggg cctcgccgct ggaagcaaag ctggtcgccg gccaggaaat
     1141 cgccctggtc ggggcgggaa actctgccgg gcaagccacc gtctatctgg cgtcgcaggc
     1201 ggccaaggtg tggctgctcg tgcgcggcag ctcgctggag gccagcatgt cgcgctatct
     1261 ggtggatcgc attcgtggcc tggagaacgt caccttggtc tgccaggccg aggtcagcgg
     1321 gcttgccggt gccaacggca tgctggagac ggtgcgctgg cggctcgcct cgggcgcggt
     1381 aatagagcgc cagatccggc acctctttct ctttattggc gctgagccca acagccactg
     1441 gctcgccggc gccctcaagc tcgacgccaa ggggttcatc ctgacgggtc gagaggccgg
     1501 cggcgccagc cggcacttgc tcgaaaccag ccgcgacggc gtatttgcga tcggcgacgt
     1561 cagagcgggc tcggtcaaac gcgtcgccgc ggcggtgggc gaaggggctc aagtcgtcgc
     1621 catgctgcac gcctaccttg ccgccagcac cggcgaaagt cagcccggca gccacactcg
     1681 cccatcgcct tagtaggcat cggcgacata gcgttccttc ggg
//
LOCUS       DASTMT010000061         4255 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_2984668, whole genome shotgun sequence.
ACCESSION   DASTMT010000061 DASTMT010000000
VERSION     DASTMT010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4255
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2984668"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..143)
                     /locus_tag="VFR00_01250"
     CDS             complement(<1..143)
                     /locus_tag="VFR00_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HEU0157902.1"
                     /translation="MNEGLSRAAYAEGKGFLSPIADILEDLRNGRMIILVDAEDRENE
                     GDLV"
     gene            complement(140..733)
                     /locus_tag="VFR00_01255"
     CDS             complement(140..733)
                     /locus_tag="VFR00_01255"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013948387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEU0157903.1"
                     /translation="MFTGIITDVGELFAREDGRFEVRSGYPAAGIAVGASIACDGVCL
                     TATDVRPAEAGSVFTADVSNETLSRTSLGSWQPGRKINLERALRAGDELGGHIVSGHV
                     DGLACIVDITPDGSSRRLLLETSDQLARYIAAKGSVALDGISLTVNEVVGRRFGINVI
                     PHTLTQTTLGIKKPGDAINLEVDLLARYVARALEYRP"
     gene            complement(870..1322)
                     /locus_tag="VFR00_01260"
     CDS             complement(870..1322)
                     /locus_tag="VFR00_01260"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEU0157904.1"
                     /translation="MTAKFRYYLRVRYQDCDAQHVVFNARYGDYIDLACSEFMRAALP
                     SPRDVFDGTFEIQTVRQVVEWKVPARFDDVLEISVWASRIGTTSFTLSFELRRASDAT
                     LLVTSETVYVHVDAKSFLKRGIEAKMRAALEAGARGKTTDHAGYLGQA"
     gene            complement(1319..2467)
                     /gene="ribD"
                     /locus_tag="VFR00_01265"
     CDS             complement(1319..2467)
                     /gene="ribD"
                     /locus_tag="VFR00_01265"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013913174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HEU0157905.1"
                     /translation="MLFASEHGVDRAAQDRRFMAVALGLARRVLGTTAPNPAVGAVIV
                     DEATGELISRGWTQPGGRPHAEAHVLERAGAQARGRTMYVTLEPCSHHGRTPPCADAI
                     LRAGIQRLVGAIEDPNPEVSGRGFASLRQAGVAVETGVCAEAARWMAGGHILRMTRGR
                     PFVQLKLAVSADALIAPGDGQPRWVTSPSARAYAHLLRARADAILIGRQTVQDDDPQL
                     TCRLPGLGHRSPRRYVLDTWFRTPGGSKLASTADAVAVTLIGALEAPQPQLPGSVATR
                     RVALEDGRVSLRAVLDLLKADGITRLLIEGGPHVAGSFVAADLVDEAIIARGTETLGA
                     AGVRPLADRGLEVFADETRWLLHSDRYLGSNRITVYRARGHLAAGRGE"
     gene            complement(2471..2980)
                     /gene="nrdR"
                     /locus_tag="VFR00_01270"
     CDS             complement(2471..2980)
                     /gene="nrdR"
                     /locus_tag="VFR00_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HEU0157906.1"
                     /translation="MRCPYCGCENTQVKDSRPTEENQAIRRRRSCEDCGGRFTTFERV
                     QLREIVVAKRSGRRVPFDREKLSRSVEVALRKRPIQPERIERMVSGIVRQVESMGEQE
                     IPSSIIGELVMEALKGLDPVAYVRFASVYRDFREASDFQEVLGEIARAPETDAPTSAP
                     AAEALERKH"
     gene            complement(3045..>4255)
                     /gene="glyA"
                     /locus_tag="VFR00_01275"
     CDS             complement(3045..>4255)
                     /gene="glyA"
                     /locus_tag="VFR00_01275"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012332914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEU0157907.1"
                     /translation="RERRRQQQEIELIASENFVSRAVLEAAGSVLTNKYAEGYPGNRY
                     YGGCQFVDEIETLAIERAKALFKCNFANVQPHSGSTANQAAFMAMMRPGDTFMGLSLD
                     TGGHLTHGSPANLSGKWFHVVPYKVRRQDHRIDMDDVRELARAHRPKVIVAGGSAYPR
                     IIDFAAFRAIADEVGALLMVDMAHFAGLVAGGAHPSPFPHAHVVTSTTHKTLRGPRGA
                     MILSNDEALAKKINSAVFPGMQGGPLMHVIAAKAVAFAEAMRPEFKAYARHVVENAKA
                     LGEVLLSAGYDLVSGGTDTHLNLVDLRPKKLTGVAAEKALGRAHITVNKNAVPFDPAK
                     PQITSGIRIGSPAGTTRGFGLAEFRSIGRLMAEVLDGLAVNGEAGNAKVEAKVREEAL
                     GLCARFPIYD"
BASE COUNT          720 a         1414 c         1400 g          721 t
ORIGIN      
        1 accaggtcgc cctcattctc gcgatcctcg gcgtcgacca ggatgatcat acggccattg
       61 cgcaaatcct cgaggatgtc cgcaatgggt gacaggaagc ctttgccctc ggcataggcg
      121 gcgcgtgata gtccttcatt cacgggcggt actccaaggc cctcgcgacg tagcgggcca
      181 agagatcgac ttccaggttt atcgcgtcgc ctggcttttt gatccccaat gtggtttggg
      241 tcaaggtatg cggaatgacg ttgatgccaa agcggcgacc aacgacctca ttgacggtga
      301 gggagatgcc gtcgagcgcc accgaaccct ttgcggcaat gtacctggcc agctgatcgg
      361 aggtttcaag caagaggcgg cgcgatgacc cgtcgggcgt gatatcgacg atgcaggcaa
      421 ggccgtcgac gtggccgctg acgatatgtc cgcctagctc gtcgccggcc ctgagcgccc
      481 gctccagatt gatcttgcgc ccgggttgcc aactccccag gctggttcgg ctcagggttt
      541 cgttcgatac gtccgccgta aagacgctgc cagcctcggc agggcggacg tcggtcgccg
      601 tcaagcacac accgtcgcag gcaatggagg cacccacggc gatgccggct gccgggtagc
      661 ccgagcgaac ttcgaagcgg ccgtcctcgc gggcgaaaag ctcgcccacg tcggtgatga
      721 tgcccgtgaa catgcctgag gctagcacac atgcacccga tccgcgaacg tgcaaagcca
      781 tgcagccttg caacgctcgg gcgcggcggg cgagggcgtc gccttgcgtc tcttgcccgt
      841 ggccggcaag acgttagcca agccaacctt taagcttggc caagatagcc ggcgtgatcg
      901 gtggtcttgc cccgggcgcc ggcctcgagc gcggcccgca tctttgcttc gatgccacgc
      961 ttgaggaagc ttttggcatc gacgtgcacg tacaccgttt cgctggtgac gagcagcgtg
     1021 gcatcgctgg cgcggcgcag ctcgaagctg agcgtaaagg acgtggtgcc gatgcggctg
     1081 gcccagaccg agatctccag cacatcgtcg aagcgcgccg ggaccttcca ttccaccact
     1141 tggcgcaccg tctggatttc gaaggtgcca tcgaacacgt cgcgcggcga ggggagcgcc
     1201 gcgcgcatga actcggagca cgcaaggtcg atgtaatcgc cataccgggc gttgaagacg
     1261 acgtgctggg cgtcgcagtc ctggtagcgc acgcgcaggt aatagcgaaa tttcgcggtc
     1321 attcgcccct gccggctgcc aggtggccgc gcgcacgata gacggtgatg cgattgctgc
     1381 ctaagtaccg atcggaatgg agcagccagc gggtctcgtc ggcgaagact tcgaggcccc
     1441 ggtccgccag tgggcgcacg cccgcggcgc ccaacgtctc tgtgccgcgc gcaatgatcg
     1501 cctcatcgac gaggtcggcg gcgacgaacg aaccggcgac gtggggccct ccctcgatca
     1561 gcaggcgggt aatgccgtcc gctttgagca ggtcgagcac cgcgcgcaac gacactctgc
     1621 cgtcctccag cgccacgcgg cgcgtggcga cgctgccggg cagttgcggc tgcggtgcct
     1681 ccagcgcgcc gatcagtgtg acggccaccg catcggcggt gctggcgagc ttgctgccgc
     1741 ccggggtgcg aaaccaggtg tcgagcacgt agcgccgggg cgagcgatgg cccaagccgg
     1801 gcaagcggca ggtcagctgc ggatcgtcat cctgcacggt ctggcggccg atcagaatag
     1861 cgtcggcgcg cgcccgcagc agatgggcgt aggcgcgtgc cgacgggctc gtcacccagc
     1921 gcggctgccc gtcgcctggg gcgatcaagg cgtcggcgga gacggcaagc ttcagctgca
     1981 cgaaggggcg cccccgcgtc atgcgcagga tgtgcccgcc cgccatccag cgcgccgcct
     2041 cggcgcacac acccgtttcc acggcgacgc ccgcctggcg caggctggca aaacccctac
     2101 ccgaaacctc agggttggga tcctcgatcg caccgaccag gcgctgaatg ccggcacgga
     2161 ggatcgcgtc ggcgcacggc ggggtgcgtc cgtggtgtga acaaggctcc agcgtgacgt
     2221 acatggtcct gccgcgggcc tgcgctcccg cccgctcaag cacgtgcgct tccgcatgcg
     2281 gccggccacc gggctgggtc cagccgcggg agatcagctc cccggtagcc tcatcgacga
     2341 tgacggcacc caccgccgga ttgggcgcgg tcgtacccaa cacgcgccgg gccaggccga
     2401 gcgccacggc catgaagcgg cgatcctgcg ccgcgcggtc aacgccatgc tcagaagcga
     2461 aaagcaacgg tcaatgcttt ctttccagcg cctcggccgc cggcgcggac gttggcgcgt
     2521 cggtctcggg tgcgcgggcg atctcgccca gcacctcctg gaaatcggag gcctcgcgga
     2581 aatcccggta gaccgaggcg aagcgcacat aggccacggg atcgagcccc ttcaacgcct
     2641 ccatcaccag ctcgccgatg atggaggagg gtatctcttg ctcgcccatg ctttcgactt
     2701 ggcgaacgat gcccgagacc atgcgctcga tgcgctcggg ttggattggc cgcttgcgca
     2761 gggcaacttc cacagatcgc gacagcttct cgcgatcgaa cggcacgcgc cgaccggacc
     2821 gcttggcaac gacgatctcg cgcaattgca cgcgctcgaa cgtggtgaag cggccaccgc
     2881 aatcctcgca gctgcggcgc cggcggatcg cctggttctc ctcggtgggg cgactatctt
     2941 tcacctgggt attctcgcaa ccgcaatatg ggcagcgcat gttccctgct cccgcctagc
     3001 ccgccgaacc cccgtggttc gctgtggctt accccaacga ggtctcaatc gtagatcgga
     3061 aagcgcgcac acagccccag cgcttcctcc ctgaccttcg cctccacctt cgcattgccg
     3121 gcctcgccgt tgaccgccag gccgtctaga acctccgcca ttaggcgtcc gatgctgcga
     3181 aactccgcca ggccgaagcc acgcgtggtg ccagccggcg agccgatgcg aatccccgaa
     3241 gtgatctgcg gcttcgccgg atcgaacggc acggcatttt tgttgaccgt gatgtgggcc
     3301 cgcccgaggg ccttttcggc ggcgacgccc gtcaattttt tcggccgcag gtcgaccaga
     3361 ttgagatggg tatcggtgcc gccggaaacg aggtcatagc cggcgctcag cagcacctcc
     3421 ccgagcgcct tggcattctc gaccacgtgg cgggcatagg ccttgaactc ggggcgcatg
     3481 gcctcggcaa atgccaccgc tttggcggca atgacatgca tcaacggacc cccctgcatg
     3541 cccgggaaaa ccgcggaatt gattttcttg gccagcgcct cgtcattgct gaggatcatg
     3601 gccccgcgcg ggccgcgcag cgtcttgtgg gtggtgctgg tgacgacgtg tgcatgcggg
     3661 aatggacttg gatgggcgcc gccggcgacc aggccggcga agtgcgccat gtccaccatc
     3721 aagagcgcgc ccacctcgtc ggcgatcgcg cgaaaggcgg caaaatcgat gatgcgcggg
     3781 taggcgctgc cgccggcaac gatgaccttc gggcggtgcg cacgcgccag ctccctgaca
     3841 tcgtccatgt cgatgcggtg gtcctggcgg cgaaccttgt agggcacgac atggaaccac
     3901 ttgcccgaca ggttggccgg cgagccgtgc gtcaagtgac ctccggtatc gaggctcagg
     3961 cccataaagg tatcgccggg tcgcatcatc gccatgaacg cggcttggtt ggcggtgctg
     4021 ccggagtgtg gttgcacatt ggcgaaattg catttgaaga gcgctttggc acgctcgatc
     4081 gccagcgtct cgatctcgtc gacaaactgg cagccgccgt agtagcggtt gccgggatag
     4141 ccttcggcgt acttgttggt gagcacgctg ccggccgcct cgagcacggc gcgcgacacg
     4201 aagttctccg aggcaatgag ctcgatttcc tgctgctggc gcctgcgctc acgtt
//
LOCUS       DASTMT010000062         5408 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_3969965, whole genome shotgun sequence.
ACCESSION   DASTMT010000062 DASTMT010000000
VERSION     DASTMT010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 5408)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5408)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5408
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3969965"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            103..534
                     /locus_tag="VFR00_01280"
     CDS             103..534
                     /locus_tag="VFR00_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013947307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU0157908.1"
                     /translation="MARVVGIDHLVLRVKEFERSRQFYDRVLGFLGFKRKYNFAKTAG
                     WSNGKTLLWIGEADAEGKKHPHRVGNIGFHHYAFELAKRQDVDDLYQLLQRYKIPVVD
                     PPADYPDYGEGYYAVFFTDPDGLKLEGMVFKPPRRRTRQRQ"
     gene            622..771
                     /locus_tag="VFR00_01285"
     CDS             622..771
                     /locus_tag="VFR00_01285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0157909.1"
                     /translation="MRYWTSQIVIGIIVTVIGTVIADLVIKTFGGRRHTGVAPHYSGP
                     ARAGR"
     gene            complement(768..1829)
                     /locus_tag="VFR00_01290"
     CDS             complement(768..1829)
                     /locus_tag="VFR00_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018321337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU0157910.1"
                     /translation="MSTAAVTAGPGDYYLPVRPDWLARRDEAILEPDLPIVDPHHHLW
                     DRPGWRYLLDELVADTSSGHNVVATVFVQARAMLRASGPVELRHVGETEFANGAAAMS
                     ASGSYGKTRHCAGIVGHADLTLGSRVEPVLEAHLRAGGDRFRGIRHSSAWDQDAQVRN
                     PAYNPPAGLLGEVAFREGFAVLSRLGLSYDAWLYHPQIDELTELARAFPASKIVLDHV
                     GGPIGIRAYAGKREEVFGRWKTSILALAACGNVHVKLGGLGMRIGGFPFHEGPEPPSS
                     DSLARAWRPYFETCIEAFGPSRAMFESNFPVDKGSYSYPVFWNACKRIAQGASASEKA
                     DLFWGTAARFYGLDLARLG"
     gene            1937..2284
                     /locus_tag="VFR00_01295"
     CDS             1937..2284
                     /locus_tag="VFR00_01295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0157911.1"
                     /translation="MPIKSKYVFVASMDVDPDKEALFNEVYDVEHVPNLLQVPGVHAV
                     SRIAGEPFAMSIGGEEKRVSHDGPRYSAIYEIDGPHVLTSAAWAKAVEAGRWPGEVRP
                     HTRNRRHALYRVR"
     gene            complement(2656..2820)
                     /locus_tag="VFR00_01300"
     CDS             complement(2656..2820)
                     /locus_tag="VFR00_01300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0157912.1"
                     /translation="MELWKGLGDELREMLWLASLVGALSVVGVGLAVLVALVLDHWQT
                     WSAAALSQLT"
     gene            complement(2894..3100)
                     /locus_tag="VFR00_01305"
     CDS             complement(2894..3100)
                     /locus_tag="VFR00_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020186372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NepR family anti-sigma factor"
                     /protein_id="HEU0157913.1"
                     /translation="MAKRKPDREQDQPAPAPADAAGSDFALHEHIGRQLRGIFDEVVT
                     QPVPEKFRKLLDELERKQSAPKRK"
     gene            3253..4062
                     /locus_tag="VFR00_01310"
     CDS             3253..4062
                     /locus_tag="VFR00_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015315397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU0157914.1"
                     /translation="MPTLAESIASHLPQLRRFARLLTGSQQAGDAAVSRMLQAIVADP
                     GTFPEKPSRVELYQQFLRTLAGIPEDSAPQGASIGQTAARSLAAMTPQARQAFLLVSV
                     EELAPREAAEVLEVSQRRAEELLTQASEEIARQIATDVLIIEDEPLIALDLQRMLEGL
                     GHRVTAIARTRDDAVEAASSNKPGLVLADIRLADGSSGLEAVNDILSSFSVPVVFITA
                     YPDKLMTGERPEPTFLITKPFREDAVKAVVSQVLFFDQQARRRHKGPAATA"
     gene            complement(4066..4632)
                     /locus_tag="VFR00_01315"
     CDS             complement(4066..4632)
                     /locus_tag="VFR00_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009450752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU0157915.1"
                     /translation="MNIHSGPLKQALLGLIPNLRAFAVSLCGDVERADDLVQETLLKA
                     WNHLESFQEGTNLRAWLFTILRNTYFSDCRRRRREVEDREGKKAAELAIHPGQQGHLD
                     MQDFRRALNLLPPDQREALVLVGAAGFSYEEAASVSGCAVGTIKSRVNRARTKLTEML
                     GVNTPREFGPDSSTEAIVARTAGGTRLP"
     gene            complement(4629..4820)
                     /locus_tag="VFR00_01320"
     CDS             complement(4629..4820)
                     /locus_tag="VFR00_01320"
                     /inference="COORDINATES: protein motif:HMM:NF037848.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NepR family anti-sigma factor"
                     /protein_id="HEU0157916.1"
                     /translation="MENRPDMQRAPRHDSQAPGMIDRSLQAQLGRQLRAIFEGIEEEP
                     VPERFVRLLEALEAREKQR"
     gene            5130..>5408
                     /locus_tag="VFR00_01325"
     CDS             5130..>5408
                     /locus_tag="VFR00_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014131210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU0157917.1"
                     /translation="MTLAEKVAAHLPYLRRFARSLTGSQKSGDAYVASTLEAILADPS
                     IFPPELPARIALYRTFLSIFNSVALNTQAPDAGSHALDAAKRNFDTLTP"
BASE COUNT          933 a         1769 c         1756 g          950 t
ORIGIN      
        1 gaatgagccc gccggcgctg cccgcccgga tctgtgccgc gcttgggcgc gttgccgcgg
       61 gcgccgggct tgtccctgcg ccggccggaa agagggcttg agatggcgcg cgtcgtcggc
      121 attgaccacc tcgtcctgcg cgtcaaggag ttcgagcgct cgcggcagtt ctacgatcgg
      181 gtgctggggt ttctgggttt caagcgcaaa tataacttcg ccaagaccgc cggctggagc
      241 aacggcaaga cactgctgtg gatcggcgag gccgatgccg aaggcaagaa gcatccccac
      301 cgcgtcggca acattggctt ccatcactac gcctttgagc tcgcaaagcg ccaagacgtc
      361 gatgacctct atcagctctt gcaaagatac aagatcccgg tggtcgatcc ccctgccgac
      421 tatccagact acggcgaggg ctactacgcg gtgttcttca ctgatccgga cggtttgaag
      481 ctcgagggaa tggtgtttaa gccgccgcgc cgccgcacgc gccagcgcca gtaacgctcg
      541 ccccgcacgt ccgtccccgc aaaccgcaat tttgccctca gcacccgcta caaggccggc
      601 agcaagctgg gcacgggaca catgcgctat tggacctcgc agatcgtgat cggcattatc
      661 gtcaccgtga tcgggacggt gattgccgat ctggtgatca aaaccttcgg cgggcgcagg
      721 cacaccggcg ttgccccgca ctattccgga cccgcccgcg ccgggcgcta gcccagccga
      781 gcaagatcca agccgtagaa acgagccgcc gtcccccaga ataagtccgc cttctcgctg
      841 gcgctggcgc cctgggcgat gcgcttgcag gcgttccaga ataccggata gctgtaggag
      901 cccttgtcga ccgggaagtt gctctcaaac atcgcacgcg atggcccgaa cgcctcgatg
      961 caggtctcga agtagggacg ccatgcccgc gccaggctgt cggaggacgg cggctcgggg
     1021 ccctcgtgga aaggaaagcc gccgatgcgc atgccgagcc caccgagctt gacatggacg
     1081 ttgccgcagg cggcgagcgc aaggatcgac gtcttccagc gcccgaacac ctcctcgcgc
     1141 ttgcccgcat aggcgcggat gccaatcggc ccgcccacgt gatcgagcac gatcttgctc
     1201 gccgggaagg cgcgggcaag ctcggtgagc tcatcgatct gtgggtgata gagccaagca
     1261 tcatagctga gaccgagcct gctcaggacg gcgaaaccct cgcgaaaagc gacctcgccg
     1321 agcaggccgg cgggagggtt ataggccggg ttgcgcacct gtgcgtcttg atcccaagcg
     1381 ctactgtggc ggatgccgcg gaagcgatcg ccaccggcgc gcagatgggc ttcgagcacg
     1441 ggctcgacgc ggctgccaag cgtgaggtcg gcgtggccga cgatgccggc gcaatggcgc
     1501 gttttgccgt agctgccgct ggcgctcatg gccgcggcac cattggcgaa ctcggtttca
     1561 ccgacgtgtc gcagctccac cggcccgctc gcccggagca tggcgcgcgc ctgcacaaac
     1621 acggtggcaa cgacattgtg gccgctgctg gtgtcggcca ccaactcgtc gagcaggtaa
     1681 cgccaacctg gccgatccca gaggtgatgg tggggatcga cgatgggcag atcgggctcg
     1741 aggatggcct cgtcgcggcg ggcaagccaa tctggtcgca ccggcagata atagtccccg
     1801 gggccggcgg taactgcagc ggtggacatt tgcgggacac tccttaaggt ttgcagccac
     1861 caccgccgga tcgggcctgt ggcctgcctc gggcgccggc aaatgatggc aaccccaagc
     1921 agcgagcgaa cttaaaatgc caatcaagtc gaagtatgtg ttcgtggcaa gcatggacgt
     1981 cgatcccgac aaggaggctt tgttcaacga agtctatgac gtcgagcatg tgccgaacct
     2041 cctgcaggtt cccggcgtgc acgccgtcag ccgcatcgcc ggagagccgt tcgccatgag
     2101 catcggcgga gaggagaagc gggtgagcca cgacgggccg cgctatagcg cgatttatga
     2161 gatcgacggc ccgcacgtgc tgacgagtgc ggcgtgggcc aaggcggtgg aagcgggacg
     2221 ctggccggga gaagtgcggc cccatacacg caaccgacgc cacgcgctct accgggtgcg
     2281 ctgacgctgg ctttgccggc acggccgggc gctctttggc gccgcccgcg cgttgcatgg
     2341 catcgacccc cgctctttcg acgccggcac cggtgccgcc cggtggcctt gagctcaaaa
     2401 gccaggcgtg ccgcggacca acaccccacg gcaagtcatg tcttgaggac gtcggccaaa
     2461 agaaatgacc cgcggcttgg ggggaccgcg ggtcaatgcg cgactggcgc gtgagggaag
     2521 cggaaggggg ggtctgcgcg cccgtctgca cagaaaaacc cgcctactgc gcgatcgttc
     2581 cgccaccctt tcaaattttt ttcggcaatt ttgccatcag agtctctgcc gaatagatct
     2641 cgtgttttca gtggcttatg taagttggct cagagccgcg gctgaccacg tctgccagtg
     2701 gtcaagcacg agggcgacga gcacggccaa gcccacgccc accaccgaca gggcgcccac
     2761 cagcgaggct agccacagca tctcgcgcag ttcgtcgccg aggcctttcc aaagttccat
     2821 gaggtcgcct cccgcagcta gtacgttggc tgctcaaccg aacgggcggc gatttcgttc
     2881 ccggttcagc ccctcacttt cgcttcggcg cggattgctt acgctccagc tcatcgagca
     2941 gtttgcggaa cttctccggc acgggctggg tgacgacctc gtcgaagatg cctctgagtt
     3001 gccggccaat gtgttcgtgc agggcaaagt cgctgccggc cgcatcggcc ggcgccgggg
     3061 ccggctggtc ctgctctcgg tcaggctttc gttttgccat ggtcatttac atcatgcacg
     3121 aacgtcctac atattgcgca acggcacgca acgctcaagg acgctgttgg agaggctcgg
     3181 ccggcgagcg atgtttgcgg gccgcgagtg acaacctttt gtagcgtcaa ttctcgaacg
     3241 cggaaggggg ccatgccgac gctggctgag agcattgcat cgcacctccc tcagttgcgc
     3301 cgctttgcgc gcctgctgac ggggagccaa caggccggcg atgcagcggt ttcgcgcatg
     3361 ctgcaggcca tcgtggccga tcctggcact ttcccggaaa aacccagccg ggtcgaactt
     3421 taccagcagt ttttgcggac cttggccggc atcccggaag attcggcgcc gcagggggcc
     3481 agtatcggac agaccgcggc ccgctccctg gcggcgatga cgccccaggc ccggcaagct
     3541 ttcctgctcg tctcggtcga ggaattggcg ccccgcgagg ccgccgaggt gttggaggtc
     3601 tcgcagcgcc gcgccgagga gctgctgacg caggcgagcg aggagatcgc ccgccagatt
     3661 gccaccgatg tgttgatcat cgaggacgag ccgctgatcg cgctcgacct tcagcgcatg
     3721 ctcgaaggtc ttggacatcg cgtgaccgcg atcgcgcgga cgcgtgatga tgccgtggag
     3781 gcggcctcct ccaacaagcc aggtctggtg ctggccgaca tccgtctggc cgacggcagc
     3841 tccgggctgg aggcggtgaa tgatattctc tccagtttca gcgtgccggt ggtgttcatt
     3901 acggcctacc ccgacaagtt gatgacgggg gaacggccgg agccgacgtt cctgatcacc
     3961 aagccgttcc gcgaggacgc cgtcaaagcg gtggtaagcc aggtgctgtt cttcgaccag
     4021 caggcgcggc ggcggcacaa gggcccagcc gccaccgctt gagcctcagg gcagccgagt
     4081 tcccccggcc gtacgcgcca cgatcgcctc agtggaggaa tcgggtccga actcgcgggg
     4141 ggtgttgacg cccagcattt ccgtcagctt ggtgcgggcc cggttgacgc ggcttttgat
     4201 ggtgccgacc gcgcagcccg acaccgaggc cgcctcctca taggaaaatc ccgcggcgcc
     4261 gaccaggacc aaagcctcgc gctggtccgg cggcagcagg ttgagcgccc ggcgaaagtc
     4321 ctgcatgtcg agatggccct gctggccagg atggatcgcg agctccgcgg ccttcttgcc
     4381 ctcgcggtct tccacctcgc gccgccgtcg ccggcagtcg gaaaaatagg tgttacggag
     4441 gatggtaaag agccaggcac gcagattggt gccttcctgg aagctctcca ggtggttcca
     4501 ggccttcagc agcgtttcct gcaccagatc gtcggcgcgc tccacgtctc cgcacagcga
     4561 cacggcaaag gcgcgcaagt tggggatgag ccccaacagc gcttgtttga gggggccgga
     4621 gtgaatgttc accgttgctt ctccctcgcc tcgagcgcct cgagcagcct gacgaagcgt
     4681 tccggcacgg gctcctcttc gatgccttcg aagatggcac gcagctgtcg gccgagttgc
     4741 gcctgcaagg agcggtcgat catgcctgga gcttggctat catggcgcgg cgcgcgctgc
     4801 atatcgggtc tattctccat ttcggcatct gcgtctgcgc actcggcatg cctcttgccc
     4861 gcggccgggc ggacccgccg ccggcccccg ccatccccct tgatgtcggc ctcgccgggg
     4921 ccggccggcc ctgggaagcc attctttccg ttcatgtttc cccctatagg tcagaggcgc
     4981 cgcctcgcgg gcagcgtgtg ccaaaaacgc ctggggcgcc cttgggttcc gccgcgggaa
     5041 ccagcaggtt ccccatcggg aactcaaggg cctgctgttg cgtttttgtg ccgataagcg
     5101 tgctgacgcg tcgagcgagg ggagtggcta tgactttggc agaaaaggtg gcagcgcacc
     5161 tgccgtattt gagacgtttc gcgcgctctc tcaccggtag ccaaaagagc ggggacgcct
     5221 acgttgcctc cacgctcgag gcgatcctcg ccgatccctc gatcttcccg cccgagctgc
     5281 cggcccggat tgcgctttac cggacctttc tcagcatctt caactcggtc gcgctcaaca
     5341 cccaggctcc cgacgcgggg tcgcacgcgc tcgacgccgc caaacgcaac ttcgatacgt
     5401 tgacgccg
//
LOCUS       DASTMT010000063         4962 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_5268521, whole genome shotgun sequence.
ACCESSION   DASTMT010000063 DASTMT010000000
VERSION     DASTMT010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4962)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4962)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4962
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5268521"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1098)
                     /locus_tag="VFR00_01330"
     CDS             complement(<1..1098)
                     /locus_tag="VFR00_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU0157918.1"
                     /translation="MGEVSPAAVNIAILAAGLLLYALIWYLTRGLPRIAKVLARLLPL
                     GLVLPAMLILSGQREAPRKEVATAPAPGAARRESAPSGGAPPPAEPKSSAAPPPAPTV
                     PPPPVSQPAPRSLPNSAPAPAPAAPAESEKEATRSLSGPPPGSPAANQGDWDVVPVFY
                     GTDRVRKDEPKRVIYSSERARRLELGHALITVPKTHQVPNIERPFAIRIPYFQVTLYE
                     GAEDPKSHFTIKEMAILTREEFIARARERVNGSRAFKDQAVVFVHGYNNSFDYALYRT
                     AQMAYDLKFDGAAFLYSWPSLGDFSGYGYDRESSLQAEPYLRQYLELVLKETGAKQVS
                     VIAHSMGNLPLLQVLRDLGPNLPPDVRLNQII"
     gene            1206..2108
                     /locus_tag="VFR00_01335"
     CDS             1206..2108
                     /locus_tag="VFR00_01335"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEU0157919.1"
                     /translation="MATRFGNRVTEPSHRLAAPLSLTAAMVLTGANVVLGKIIVAEVP
                     VYVFMLFRFLVSTVALLALVHREGGPKLRALRGRQLRDLTCMALFGMIGFTALMFEGL
                     KRTSASDAGIITATLPAVVALLGLAVMRERLRLPQAAAIGLAVAGLLLVAAGSQRGTQ
                     SLFGNLLVVGAVACEASFVLLGKRLTPPYRPLRLALGANLVGLAGAAPLALLSSPFDL
                     GGVSPLMWLLATWYGLSASVFCLWLWYRGLPYVETWLAGLTTAAVPVTALTAAALVLG
                     ETLDAWKLAGGALVLAAIVLGALS"
     gene            2192..3217
                     /gene="meaB"
                     /locus_tag="VFR00_01340"
     CDS             2192..3217
                     /gene="meaB"
                     /locus_tag="VFR00_01340"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HEU0157920.1"
                     /translation="MSIAGQPQQVSLDAYVAGVRAGDRAMLARAITLIESSKIEHATL
                     ADALLQRLLPLTGGAMRIGITGVPGAGKSTAIDQLGVNLIEAGHRVAVLAVDPTSTRS
                     GGSILADKTRMARLTQSDRAFIRPSPTSGTLGGVARKTRETMALLEAAGFDVVLVETV
                     GVGQSEIAVAAMVDFFLVLLLAGGGDEFQGIKRGIIEIADMLAITKADGDNRERVLKA
                     ASIYQGALAILTPPSPNWTPPVVTISARENRGLDELWGRILEHRRILQASGEFVARRG
                     AQAVDWMRALLAEQLEKALRTDPQLETRVRTLEDEVRAGHLTPALAAKRLAALMGVRV
                     TPAPGAA"
     gene            3249..3893
                     /locus_tag="VFR00_01345"
     CDS             3249..3893
                     /locus_tag="VFR00_01345"
                     /inference="COORDINATES: protein motif:HMM:NF013624.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HEU0157921.1"
                     /translation="MRVLLTGFAPFPGVPVNATMALVPELAAEARRRFYGIETSAALL
                     STEWQAAPLQLEELLGELRPDLILHFGVSQRARGFEIERRGQNRCLAHPDAVGALPSS
                     LEVRAGGPDVLPASLPVSYIVARLRSRRIPAFASRDAGGYLCNATLYNSLAAARAHPG
                     RRVGFIHVPASLARAGASKGRHASSSPLSWAQALEGGQEILGACLHRLAAAGRS"
     gene            complement(3968..>4962)
                     /locus_tag="VFR00_01350"
     CDS             complement(3968..>4962)
                     /locus_tag="VFR00_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020186054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEU0157922.1"
                     /translation="LVELTRGRQRESVHTGALAVARSNRELVAFIGNVEQPIYARSAI
                     KPLQALPLLETGAADRYGFGPRELAIACASHAGTPEHTATVANMLTRVGLDSMALGCG
                     TQEPLDANASRALIRAGQAVTALHHNCSGKHAAMLATAAHKTEPTAGYWLPDHPVQTR
                     ILCTLEDMIGRRLGSDVAGIDGCSVPNWAIPLADLASAFARFVTGEGLTQARRHACQR
                     LAEACWAEPWLVGGQGRLDSVAMGKLPGKVLVKAGAEGVYCGAFAAAGLGFALKIDDG
                     GKRAADLVAANLLRQFCPEATDLGSARPLHNFRGLTVGEIRAAGALERLLGELG"
BASE COUNT          726 a         1630 c         1720 g          886 t
ORIGIN      
        1 gatgatctga ttgagcctga cgtcgggcgg caagttcgga cccaagtcgc gcagcacctg
       61 gagcagcggc aggtttccca tgctgtgggc gatgacgctc acctgtttgg cgccagtttc
      121 cttcaggacc agctcgagat actggcgcag gtagggctcc gcctgcagcg agctttcgcg
      181 atcatagcca taaccgctga agtcgccgag cgagggccag ctgtagagga aggccgcacc
      241 gtcgaatttg aggtcgtagg ccatttgcgc ggtgcgatag agcgcgtagt cgaacgaatt
      301 gttgtagccg tgcacgaaca ccacagcctg atctttgaag gcgcgactgc cgttcacgcg
      361 ctcgcgcgcc ctggcaatga actcctcgcg cgtgagtatc gccatctcct tgatcgtgaa
      421 atggcttttg gggtcctcgg caccctcgta cagggtcacc tggaaatagg ggatgcggat
      481 ggcgaacggg cgctcgatgt tgggaacttg atgcgtcttg gggacggtta ttaaggcgtg
      541 accgagctcg agccgacgcg cgcgctcgga gctatagatg acgcgcttgg gttcatcttt
      601 gcgtacgcga tcggtgccat agaaaacggg caccacgtcc cagtcgcctt ggttcgccgc
      661 cggcgagccg ggcggtggac cactgagcga gcgcgtcgcc tccttttcgg attccgcggg
      721 agcggccggc gcgggtgcag gtgcggagtt cggcagcgag cggggtgctg gttgcgacac
      781 gggtggcggc ggcacggtcg gcgctggcgg cggcgccgcc gaggacttgg gttcggctgg
      841 cggcggtgcc ccgcccgacg gcgccgactc gcggcgcgcg gctcccggcg ccggcgcggt
      901 cgccacctcc ttgcgcgggg cctcacgctg gcccgacaaa atgagcatgg ccggcagcac
      961 cagccccagt ggcagcaggc gcgccagcac cttggcaatt cgcggcaagc ccctcgtcaa
     1021 ataccagatc agggcgtaga gaagcaggcc cgcagcgaga atggcgatgt tgacggctgc
     1081 cggcgaaacc tcgcccatga tggcctcccc tctcctcctc cacctggcgc caaagagttt
     1141 acgagaaaca ccagcaccgc tgcaatcgta gcaatcccga cggcaggcgc cggcggcagc
     1201 accctgtggc aacgagattt ggcaaccgcg tgaccgagcc ctcgcaccga ctggcggcgc
     1261 cgctgtcatt gacggcggcc atggttttga ccggcgccaa cgtggtgctg ggcaaaatta
     1321 tcgtcgcgga ggtgcccgtc tacgttttca tgctttttcg cttcctggtg tccacggtcg
     1381 cgctgctagc gctcgtccat cgcgaggggg gccccaagct cagagcgctg cgcgggcggc
     1441 agcttcgcga cctcacgtgc atggcgctgt tcggcatgat cggcttcacg gctttgatgt
     1501 ttgagggctt aaagcgcacg tccgcctcgg atgccggcat cattaccgcc accctgccgg
     1561 cggtggttgc cctgttgggg ctcgccgtca tgcgcgaacg cttgcgcctg ccgcaggcgg
     1621 cggccatcgg gctcgccgtt gccggcctgc tgctggtggc cgcgggcagc cagcgcggca
     1681 cgcagagtct cttcggcaac cttctcgtcg tcggcgcggt tgcctgtgag gcaagctttg
     1741 tgctcctggg caagcggctg acgcccccct atcggccgct gcggctggcg ctgggtgcca
     1801 acttggttgg attggccggc gccgcgccgc tggcgttgct gtcgagcccc ttcgacctcg
     1861 gcggggtctc cccgctaatg tggctgctgg cgacctggta tggcctctct gccagcgtgt
     1921 tctgcttgtg gctgtggtat cgcggactgc cctacgtgga aacctggttg gcggggctga
     1981 cgacggctgc cgttccggtg acagcgctga cggcggcggc gctggtgctc ggcgaaacgc
     2041 tcgatgcctg gaagcttgcg ggcggcgccc tcgtgctggc ggccatcgtt ttgggcgcgc
     2101 tgtcctagcg cgctaccgcg ggcgggacca agcgcaggct tgcgggcggg gcgaattttc
     2161 tcgatcctat tcgtcaggag agcgcttgcc gatgtcaatt gccgggcaac cgcagcaggt
     2221 ctcgcttgat gcctatgtcg ccggcgtgcg tgccggcgac cgcgccatgc ttgcgcgtgc
     2281 gatcacgctg atcgagagtt cgaagatcga gcacgccacc ttggccgatg cgctgctgca
     2341 gcgcctcttg ccgctgaccg gcggggcgat gcgtatcggt atcaccggcg tgcccggtgc
     2401 cggcaagtcc accgccatcg atcagcttgg cgtcaatctg atcgaggcgg gccatcgtgt
     2461 cgccgtgctg gccgtcgacc ccacctcgac gcgatcgggc ggctcgatcc tggccgacaa
     2521 gacccgcatg gcgcgcctga cgcaaagcga tcgggccttc attcgccctt cgcccacctc
     2581 gggcacgctg ggtggtgtgg cgcgcaagac ccgcgagacg atggcgctgt tggaagcggc
     2641 ggggttcgat gtggtgttgg tggagaccgt cggcgtcggc caatcggaga tcgcggtcgc
     2701 cgcgatggtc gatttctttc tcgttcttct actcgccggc ggcggcgacg agtttcaggg
     2761 tatcaagcga gggatcattg aaattgccga tatgctggcc atcaccaagg ccgacggcga
     2821 caaccgggag cgtgtgttga aagccgcctc gatctaccaa ggggcgctgg cgatcctgac
     2881 accaccctcg ccgaattgga cgccgcccgt cgtcaccatc tccgcccgcg aaaatcgcgg
     2941 gctcgacgaa ttatggggcc ggatcctgga gcaccgccgc atcctgcagg cgagcggtga
     3001 gtttgtggcc cggcgcggag cccaagccgt cgattggatg cgcgcgctcc tggccgagca
     3061 attggaaaag gcgttgcgca ccgacccgca gcttgaaaca cgcgtgcgca cgctcgagga
     3121 cgaggtgcgc gcgggtcatc tcacgcccgc gcttgcggcc aagcggcttg ccgcgttaat
     3181 gggtgtgcgg gtgacgccag cgccgggcgc tgcataaggt ctaagagggc ttgggcgcgg
     3241 acatggccat gcgcgtgcta ctcaccggtt ttgccccttt tcccggcgtg cccgtcaacg
     3301 cgaccatggc gttggtgccg gagctcgccg ccgaggcgcg acgccgattt tacggcatcg
     3361 agacgagcgc ggccctgctg tcgaccgaat ggcaagccgc ccccctccag ctcgaggagc
     3421 tgctgggcga gttgcggccc gacctaatcc tgcatttcgg cgtctcgcaa cgcgcgcggg
     3481 gctttgagat cgagcggcgc gggcaaaacc gctgccttgc ccaccccgac gcggtcggtg
     3541 ccttgccttc ctctctcgag gtgcgcgccg gcgggccgga cgtgctgccg gccagcctgc
     3601 ccgtcagcta catcgtggcg cgcttgcgtt cgcgtcgtat tccggccttt gcttcgcgcg
     3661 acgcgggtgg ttatttgtgc aatgccacgc tttataattc gctggcagcg gcgcgcgccc
     3721 accctggccg ccgcgtcggc tttatccacg ttccagccag cctggcacgc gcgggcgcct
     3781 cgaaagggcg ccacgccagt agctccccgc tgagctgggc gcaggcgctg gagggcgggc
     3841 aagaaatcct tggcgcctgt ctgcaccggc ttgctgcagc cgggcggtcg tagcggtcgt
     3901 cttgaggcct cgctgtcgtc agcttcgccg gcgtgcgacc gaccccattt agagagccgc
     3961 ggcagcgtca gccaagctcg ccaagcaaac gctcgagcgc gccggcggcg cgtatttcgc
     4021 cgaccgtcag gccacggaag ttatgcagcg gccttgccga gccgaggtct gtcgcctcgg
     4081 gacagaactg acgcagcagg ttggcggcaa ccaggtccgc ggcgcgtttg ccgccgtcat
     4141 cgatcttgag cgcgaagccg aggcccgctg ctgcaaaggc gccgcagtaa acgccttccg
     4201 cgcccgcctt gacgaggacc ttgccgggca gtttgcccat ggccacgcta tcgagccggc
     4261 cctggccgcc caccagccag ggctcggccc agcaggcctc cgccagtcgt tgacacgcgt
     4321 gccggcgcgc ctgcgtcagg ccttcacccg tcacgaagcg cgcgaaggcg ctggcgaggt
     4381 cggccagcgg aatggcccag ttcggcaccg agcagccgtc gatgccggcc acgtcgctgc
     4441 cgagccgacg gccgatcatg tcttcgagcg tgcacaggat gcgcgtttgc acggggtgat
     4501 cgggcagcca gtagccggcg gtcggttcgg tcttgtgcgc cgccgtggcc agcattgcgg
     4561 cgtgcttgcc cgagcagttg tgatgcagcg ccgtgacagc ctggccggcg cgaatcagcg
     4621 cacggctggc gttcgcatcc aatggctcct gcgtgccaca ccccaaggcc atgctatcga
     4681 ggcctacccg cgtcagcatg ttggcgaccg tggcggtgtg ttcgggggtg cccgcgtgcg
     4741 aggcgcaggc aatggccagc tcgcgcggcc cgaagccgta gcggtccgcc gcgccggtct
     4801 ccagcagcgg cagggcctgc aagggtttga tggccgaacg cgcgtagatc ggttgctcga
     4861 cgttgccgat aaaagccacg agttctcggt tggagcgtgc gacggcaagc gcgccggtgt
     4921 gcacgctctc gcgctgccgg ccgcgcgtaa gctcgacaag aa
//
LOCUS       DASTMT010000064         2366 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_34365, whole genome shotgun sequence.
ACCESSION   DASTMT010000064 DASTMT010000000
VERSION     DASTMT010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2366)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2366)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2366
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34365"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1010)
                     /locus_tag="VFR00_01355"
     CDS             complement(<1..1010)
                     /locus_tag="VFR00_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HEU0157923.1"
                     /translation="MKTETPRPILLKDYRPPNYLIDHVDLNVALAPERTRVHARLQLR
                     PNPAFEGELGPLRLDGEMLELESARLDGRALTPADYSLSDKELVIGKAPAHAFLLDLV
                     TFCNPEANKALSGLYRSRGIYCTQCEAQGFRRITYFLDRPDVLATYTTRLEAPGAEAP
                     VLLSNGNPTEQGVLDGGKRHFAVWHDPHPKPSYLFALVGGDLAHLASDFETASGRKVA
                     LKIYVEPGKEGRCAWAMDALKRAMRWDEERFGREYDLDVFNIVAVSDFNMGAMENKGL
                     NIFNDALVLASPETASDQSFADIERVIAHEYFHNWTGNRITCRDWFQLCLKEGLTVFR
                     DQ"
     gene            complement(1217..1999)
                     /locus_tag="VFR00_01360"
     CDS             complement(1217..1999)
                     /locus_tag="VFR00_01360"
                     /inference="COORDINATES: protein motif:HMM:NF025050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU0157924.1"
                     /translation="MIYALEQVSIAVSATRLAEAARAYATLLGRRAQQHPAGCSFQLS
                     NVRLELVADAAAPGLAGLAFAVEDLEAWGRLLARRGLPVQPANLGDQSAPPALLADRQ
                     ATLGVPMHFCAGEKEAVRPSTPTLPETETTAVTALDHLVIKTPNPDRAIALYAGRLGL
                     SLRLDRSDAEWGARLLFFRCGALTVELVHRPGQESGEGVDRLWGLSWRVPDMAGAHAR
                     LRAAGVDLSEIRGGRRPGTRVATVRSHTAGVATLLIGPDAGA"
BASE COUNT          395 a          762 c          840 g          369 t
ORIGIN      
        1 tcctgatcgc ggaagaccgt caacccctcc ttgaggcaga gctggaacca gtcccggcag
       61 gtgatgcggt tgcccgtcca attgtggaag tactcgtgtg cgatcacgcg ctcgatgtcg
      121 gcgaaactct ggtcgctggc ggtttccggt gaggccaaga ccagggcgtc gttgaagatg
      181 ttgaggccct tgttctccat ggcgcccatg ttgaaatcgg acaccgccac gatgttgaag
      241 acatcgaggt catattccct gccgaaccgc tcctcatccc agcgcatggc gcgcttgagc
      301 gcgtccatgg cccaggcgca gcggccctcc ttgcccggct ccacgtagat tttgagcgca
      361 accttgcggc cggaggcggt ctcgaaatcg gaggccaagt gcgccaggtc gccacccacg
      421 agggcgaaca ggtagctcgg cttgggatga ggatcgtgcc agacggcaaa atggcgcttg
      481 ccgccatcaa gcacgccctg ctcggtcgga ttgccgttgg acagcaagac cggcgcctcg
      541 gcgccgggcg cttcgagccg cgtggtatag gtcgccagca cgtcggggcg atcgaggaaa
      601 taggtgatgc gccggaagcc ttgcgcttcg cactgcgtgc aataaatgcc gcgcgagcga
      661 tagagcccgg aaagtgcctt gttggcctct gggttgcaga aggtgacgag gtcgaggagg
      721 aaagcgtggg cgggcgcctt gccgatcacc agctccttgt cgcttaggct atagtcggcg
      781 ggcgtcagtg cgcggccgtc gagccgggcg ctctccagct cgagcatttc gccatcgagg
      841 cgcaagggcc cgagctcgcc ctcgaaggcc ggattgggcc gcagctgcag gcgggcatgc
      901 acgcgcgtgc gctcgggggc gagcgcaacg ttaagatcga cgtgatcgat aaggtagttc
      961 ggcgggcgat agtccttgag gaggatcggc cgcggcgttt cggttttcat gcgtgtcctg
     1021 ggctcaagag tgcgcaagcc cgctgccgtc ggccgggcgt taggtccttc ttagtgcaat
     1081 gcggcggggg cgcaagttat gacgcacgac cgggaacgcg tttggctgtt tgccgcaaac
     1141 cgcaggcggc ggcaagggaa gcgccgctac gtcacgccgc gccaggccaa tgggccgcag
     1201 ctctggggac aggcaattag gcgccggcgt ccggcccgat caacagggtt gcaacccccg
     1261 cggtgtggga gcgcaccgtt gccacccgcg ttcccggccg acgcccgccg cggatctccg
     1321 acaggtcgac ccctgccgcg cgcaggcggg catgcgcgcc cgccatgtcg gggacccgcc
     1381 acgagaggcc ccataggcga tccacgccct cgccgctctc ctgccccggc cgatgcacca
     1441 gctcgacggt gagcgcgccg cagcggaaga acaacaggcg cgcaccccac tcggcatcac
     1501 tgcggtcgag gcgcaaggaa aggcccaagc gtccggcgta aagggcgatg gcgcgatcgg
     1561 ggttgggcgt cttgatcacc agatgatcaa gcgccgtgac ggccgtcgtc tcggtctccg
     1621 gcagcgtcgg cgtgctcggc cgcaccgcct ctttctcccc ggcgcaaaag tgcatgggca
     1681 cgcccaaggt cgcctggcga tcggccaaca gggcaggggg agcgctttga tcgcccaggt
     1741 ttgccggctg gacgggcaaa ccgcgccggg cgaggagacg cccccaggcc tcgagatcct
     1801 caaccgcgaa ggcgaggcca gcgaggccgg gcgccgccgc atccgcgacg agctcgaggc
     1861 gcacgttcga gagctgaaaa gagcaccctg ccgggtgctg ctgagcccgg cgcccgagca
     1921 gggttgcgta cgcgcgcgcc gcttcggcga ggcgcgtggc cgagacggcg attgagacct
     1981 gctcgagggc ataaatcatg tttggcgcgg ccgaacgtcg ggacaaggcc tgggaagtgt
     2041 gcaggggcgg gcgaaaagcg caatgggcca ggcgagctgc agccgccacg gcgctcggtt
     2101 cccgcgccgg gttctcttca gcgcttgccc ggcgggtcaa tgcgttgctg ccggtcgggc
     2161 ccggccgcgg cccgcagcgc ccttgagccg acgcgggcgg cacgcgtttg gccgcgctgc
     2221 gatcaagctt cgggaatagc gctagccccg tgccgcgcca acaccgatgc gccgcatcga
     2281 cgccaagggg cctcttctgc taactgagcc gggcgcgtgt cgcggcgact tggagttttc
     2341 agatgaaggc gatgttgagc aaaagc
//
LOCUS       DASTMT010000065         8268 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_3320187, whole genome shotgun sequence.
ACCESSION   DASTMT010000065 DASTMT010000000
VERSION     DASTMT010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 8268)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8268)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8268
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3320187"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            104..1486
                     /gene="miaB"
                     /locus_tag="VFR00_01365"
     CDS             104..1486
                     /gene="miaB"
                     /locus_tag="VFR00_01365"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013418851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HEU0157925.1"
                     /translation="MAATKKVFIKTFGCQMNVYDSERIGEALGRVGYLATGRIEEADM
                     VVLNTCHIREKAAEKVYSELGRIRQLKAERGAQGKDTLITVAGCVAQAEGNEIVRRAP
                     VVDLVVGPQSYDRLGDLIERRRQERRPLVETQFPLESKFQRLPERSRQRRPSAFLTVQ
                     EGCDKFCTFCVVPYTRGAEYSRPVAEIEAEARHLLEAGARELTLLGQNVNAYRGLGPT
                     GARFSLADLIRHLARLEKLERLRYTTSHPGDMSDDLIGAHADVAKLMPHLHLPFQSGS
                     DRILAAMNRKHGIADYLGLVERLRAARPDIALSSDIIVGFPGETEADFAATLALTERV
                     GFAQAYAFKYSPRPGTPASRLGEVVPEAEKMRRLTRLQALLGRQQTSFNLASVGRTMP
                     VLFEREGQRGGQVVGRSPYLQAVYAPGAVRHLGALLDVEIVAAGPNSLSGLILEPRRG
                     GHDAETRPVC"
     gene            1550..2530
                     /locus_tag="VFR00_01370"
     CDS             1550..2530
                     /locus_tag="VFR00_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEU0157926.1"
                     /translation="MRGPGAAGLGKQLRNEQARLVVPFEDNRHLNRLLGEYDGHLALL
                     EERLGIEAHAHGNVVTLTGPAPACETAREVLEKLYARIRQGEDIGPGDVDGLLRHSRA
                     DAAPTSALAQIRTRRKVVTARSPNQGAYIEALNRVDLVFGLGPAGTGKTYLAVAYAAQ
                     CLERGQVERLVLSRPAVEAGERLGFLPGDMREKVDPYLRPLYDALYDVLPPAKVERDL
                     TTGVIEIAPLAFMRGRTLAHSFVILDEAQNTTSMQMKMFLTRLGEGSKMVVTGDPSQI
                     DLPHGQRSGLEEAVGLLNGISGIEAVRFTSADVVRRDLVARIVDAYERLR"
     gene            2538..3104
                     /gene="ybeY"
                     /locus_tag="VFR00_01375"
     CDS             2538..3104
                     /gene="ybeY"
                     /locus_tag="VFR00_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013891278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEU0157927.1"
                     /translation="MTPLADSAALPLDAEASPEPEPDGLCLSISHEEGTWEAIADLEP
                     AIRRVGEALAHHPAGVPVRGREASVVLGSDSLLRRLNASYRGKDAPTNVLSFPFKPRA
                     ARADQEADRYLGDVVLAAETIAREAAERHIEPIQHVQHLVLHGLLHLIGYEHAADAEA
                     LVMERLEAEILSRLGLPDPYAADPPNQR"
     gene            3133..4179
                     /locus_tag="VFR00_01380"
     CDS             3133..4179
                     /locus_tag="VFR00_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HEU0157928.1"
                     /translation="MSVKSTSAKPEPLTTASQDAVEPGHGWLSTLRARLGIPGAPTLR
                     ATLEAELKSAGDGHAFSAEEREMLLRILRFGALRVHDVMVPRADIIAVDENEPIRELL
                     RTFDVAGVSRVPLFRESLDDPRGMVHVKDLLRWLYGDATGRPADGNGAASRGPPAEER
                     PAAASAEPSLDLARTDLSKPIASTKLRRPMIYVPPSMPAANLLIRMQSTHIHMALVVD
                     EYGGTDGLVTIEDLVEQIVGDIEDEHDEVEAANIVSDAKLGLLTAARTPVRELEQHLG
                     IQLLKEDEEADIDTLGGLVFALVGRVPARGELIRHPAGVEFEVLDADPRRVKKLRVHV
                     TRQPAAAEASIAKS"
     gene            4166..5890
                     /gene="lnt"
                     /locus_tag="VFR00_01385"
     CDS             4166..5890
                     /gene="lnt"
                     /locus_tag="VFR00_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011500668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HEU0157929.1"
                     /translation="MPNPEPSRRDAGEPAPRGLAHILCNLTGWSRRLSHLSGWRRFAA
                     AFVAGLASVTAIAPFHAWPVLYLTLPALIWLLDGACAPAAAFKGAPKPAAAWRGVRAA
                     AAVGWWFGFGYFVVGLYWVGAAFLVEADVFAPLLPLAVTLLPAGLALFYAGAAALARP
                     FWRTGPTRVLALALALSAMEYARGHLFTGFPWNVLGYALTYPLSLMQSAALVGIYGLT
                     LLGVLIWAGPLVLLAEASPGRMGRGRRRSALALAVAPVLVLAIWGHLRLAAAPAAMLP
                     GVKLRIVQPSVPQAEKWRPDNQERIFLDHLRLSASTAAGVPDELAGITHVIWPEAAMP
                     FLPVDHPAARAAIGRLLPPGTLLIAGGLRAEADPEAPRGRRIFNSLLVFGPGGSLLAS
                     YDKAHLVPFGEYLPLASVLEALGLETLTRRRGGFARGPTPRPVLHLPGLPPVLPLICY
                     EVIFPSAVNAGSERPGLLVNLTNDGWFGNTTGPRQHMQQARVRAVEAGLPLLRAANNG
                     ISGLIDAHGRVLVELRLDQRGSADTGLPAALPPTPYARFGDAGFAGLWLLGVGLLGLG
                     RRRRRPPA"
     gene            5998..6465
                     /locus_tag="VFR00_01390"
     CDS             5998..6465
                     /locus_tag="VFR00_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU0157930.1"
                     /translation="MSRPSEFAAEADGEEKGSRRPNPIDVHVGSRVRFRRMLLGMSQE
                     KLGEKLGLTFQQIQKYEKGINRIGASRLFDLAQVLGVSVQFFYEEAPTGEVPRFVADG
                     FAERPAESSIVEFLRSRDGLELNKAFVRIADVRARRAIVELVRSLAADDDLLE"
     gene            6580..7788
                     /gene="metK"
                     /locus_tag="VFR00_01395"
     CDS             6580..7788
                     /gene="metK"
                     /locus_tag="VFR00_01395"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006609479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEU0157931.1"
                     /translation="MGNTVSRKSYLFTSESVSEGPPDKVCDRISDEVVDLFFREGPKA
                     GVDPWQLRVACETLATTNQVVIAGETRGPKSITRDLVAHVARMAIKDIGYEQEGFHWD
                     RADIRVLLHPQSADIAQGVDSGGNRDEGAGDQGIMFGFACRDTPELMPAPIYYSHKIL
                     RLISEARHAGKEAVLGPDSKSQVTVRYENGKPVAATQIVVSHQHLVEAMTSAQVRELV
                     EPYVKKALPSGWISNDTIWHVNPTGKFHIGGPDGDSGLTGRKIIVDTYGGAAPHGGGA
                     FSGKDPTKVDRSAAYAARYLAKNVVAAGLADQCTIQLSYAIGVAKPLSIYVDVGANPS
                     GIDTPKLERVLGELMDLSPRGIRKHLELNKPIYARTAAYGHFGREPDNEGGFSWEKTD
                     LGPKIKAAMA"
     gene            7853..>8268
                     /locus_tag="VFR00_01400"
     CDS             7853..>8268
                     /locus_tag="VFR00_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HEU0157932.1"
                     /translation="MLAGADELRSFGRRRGRALSPRQAGLWRELLPRVAVPLQGAALA
                     NPLDLFAGNVREVWLEIGFGGGEHLLWQAERHPEAGILGCEPFQDGVVKVLAALAERD
                     GGNIRVHADDARPLIKQLPAASVARVFILFPDPWPKR"
BASE COUNT         1282 a         2809 c         2761 g         1416 t
ORIGIN      
        1 gggcgcaaat aatttgccgc cggtgggtgg gaagggccgg cgcgcacggg ctttgtgccc
       61 ggcatggtcg cccgcccccg tcgcttctga aaagttggcg tagatggccg caaccaagaa
      121 ggtgttcatc aagacgttcg gctgccagat gaacgtctat gattccgagc gcatcggcga
      181 ggcgctcggc cgtgtcggct acctggcgac ggggcgcatc gaggaggccg acatggtcgt
      241 cctcaatacc tgccatattc gcgagaaggc ggcggagaaa gtctattccg agctcggccg
      301 cattcgccag ctgaaggccg agcgtggcgc ccagggcaaa gataccctga tcaccgtggc
      361 gggctgcgtg gcgcaggccg aaggcaatga aattgtgcgc cgtgccccgg tggtggatct
      421 cgtcgtcggc ccacagagtt atgatcggct gggcgatctc atcgagcgca ggcgccagga
      481 gcggcgccca ttggtcgaga cccaattccc cctggagagc aagtttcagc gcttgcccga
      541 gcgcagccgc cagcgccggc cgtcggcctt tttgaccgtg caggaaggtt gcgacaaatt
      601 ctgcacgttt tgcgtggtgc cctatacgcg cggggccgag tactcacgcc cggtcgcgga
      661 gatcgaggcg gaggcgcgcc atctcctcga ggctggcgcc cgcgagctca ccctgttggg
      721 ccagaacgtc aatgcctacc ggggcctcgg gccgaccggg gcacgctttt ccttggccga
      781 tttgatccgt catctggctc ggctcgaaaa gcttgagcgc ctgcgctata ccacgagcca
      841 tcccggtgac atgagcgatg acctgatcgg cgcgcacgcc gacgtcgcca aactgatgcc
      901 gcacctgcat ctgccctttc agtccgggtc ggaccgcatt ctggcggcca tgaaccgcaa
      961 gcacgggatt gccgactacc ttggcctcgt cgagcgcctg cgcgccgcgc gccccgacat
     1021 cgccctctcc agcgacatca tcgtcggctt cccgggcgag acggaggctg actttgctgc
     1081 caccctggcg ctcaccgagc gcgtcggctt tgcccaagcc tacgccttca agtacagtcc
     1141 gcgcccgggc acgccggcga gccgcttggg cgaggtggtc cccgaggccg aaaagatgcg
     1201 tcgcctgacg cggctgcaag cgcttttggg gcggcagcaa acatcgttca atttggcctc
     1261 ggtgggccgc actatgcccg tgctgttcga gcgcgagggc caacgcggcg gccaggttgt
     1321 cgggcgctcg ccctacctgc aggcggtcta tgcgccggga gcggtcaggc accttggggc
     1381 gctgctcgac gttgaaattg tggctgcggg acccaatagt ctcagcggtt tgatcttgga
     1441 gccgaggcgt ggggggcatg acgccgaaac ccgccccgtg tgctagactt gggagacgag
     1501 agattctctg cggttgggcg ttcttaagga ggccaggtct ttgaccggca tgcgtgggcc
     1561 aggggcggcc ggactgggca agcagctcag gaatgagcaa gcccgcctgg tggtgccgtt
     1621 cgaagacaat cggcatctca atcggctgct tggagaatac gacggccatc tggcgctgct
     1681 ggaggagcgg ctcgggatcg aggcccatgc ccacggcaac gtcgtgaccc tgaccgggcc
     1741 ggcgcccgcc tgcgagaccg cgcgcgaggt tctcgaaaag ctctatgccc gcatccgcca
     1801 gggcgaggac atcggtcccg gcgacgtcga cgggcttttg cgccattcgc gggccgatgc
     1861 cgcgcccacc agcgcgctgg cgcagatcag gacgcggcgc aaggtggtaa cggcgcgttc
     1921 gcccaatcag ggtgcctaca tcgaggcgct caatcgcgtc gatctggtgt ttggcttggg
     1981 gcccgccggc actggcaaaa cctatctggc cgtcgcctac gccgcccagt gcctggagcg
     2041 cggccaggtc gagcggctgg tgctgtcgcg gccggcggtg gaagccggcg agcgcctggg
     2101 gttcctgccc ggggacatgc gcgagaaggt cgatccctat ctgcggcccc tctacgatgc
     2161 gctctacgac gtcctgcccc cggccaaggt cgagcgcgac ttgaccacag gggtgatcga
     2221 aatcgcgccg ctggccttca tgcgcgggcg cacgctggcg cactcgttcg tcatcctcga
     2281 cgaagcccag aatacgacga gcatgcaaat gaagatgttc ctgacgcgtc tcggcgaggg
     2341 atcgaaaatg gtggtcaccg gcgacccaag ccagatcgac ttgccgcacg ggcagagatc
     2401 ggggctggag gaggcggtcg gactgctcaa cggcatcagc ggcatcgagg cggtgcgctt
     2461 cacgtccgcc gacgtcgtgc gccgcgacct cgtggcgcgc atcgtcgatg cttacgagcg
     2521 gctgcgttag gcgcgccatg acccccctcg ccgatagcgc cgccctgccg ctcgatgcgg
     2581 aagcctcgcc ggagcccgag cctgacggcc tctgcctctc gatcagccac gaggagggga
     2641 cttgggaggc gatcgcggac ctggagcccg ccatccgcag ggtgggcgag gccctcgccc
     2701 accatcccgc tggcgtgccc gtgcggggcc gggaggccag cgtcgtgctc ggcagcgaca
     2761 gcctgctgcg ccgtttgaac gccagctatc gcggcaagga tgccccaacc aacgtgttgt
     2821 catttccctt caagccgcgc gccgcccggg cagaccagga agccgatcgc tacctgggcg
     2881 acgtcgtgct ggccgccgag accatcgccc gggaggccgc cgagcgccac atcgagccga
     2941 tccagcacgt gcagcatctc gttctgcacg gccttctgca cctcatcggc tacgagcatg
     3001 ctgcggacgc tgaggcactc gtcatggagc ggctggaagc ggagattttg agccggctcg
     3061 gcctcccgga tccctacgcc gccgacccac ccaaccagcg ttaattttcc ctttgcaggc
     3121 cctgcgcacg ccatgagcgt caaatccacc agcgccaagc ccgaacccct aacaaccgcc
     3181 agccaagacg cggtcgaacc gggacacgga tggctctcca cgcttcgcgc ccggctcggg
     3241 atcccggggg cgccgacgct gcgcgccacg ctcgaagcgg aactgaaaag cgccggcgac
     3301 ggtcatgcct tctcggccga ggagcgcgag atgctgctgc gcatcttgcg cttcggcgct
     3361 ttgcgcgtgc acgacgtcat ggtgccgcgg gccgacatca ttgcggtcga cgagaacgag
     3421 ccgatccgcg agcttttgcg caccttcgac gtggcggggg tgtcgcgcgt accgctgttt
     3481 cgtgaatccc tcgacgatcc gcgcgggatg gtgcacgtca aggacctctt gcgctggctt
     3541 tacggcgacg ccacggggcg ccctgccgac ggtaacggtg cggcaagccg aggcccgccg
     3601 gccgaggagc ggcccgccgc cgcatcggcc gagccctccc tcgacctggc gcgcaccgac
     3661 ctgtcgaagc cgattgcctc gacgaagttg cgccgtccca tgatttacgt gccgccatcg
     3721 atgccggcgg cgaacctctt gatccgcatg cagtcgacgc acattcatat ggccctggtc
     3781 gtcgatgaat acggcggcac cgacgggctc gtgacgatcg aggatctggt cgagcagatc
     3841 gtgggcgata tcgaggatga gcacgacgag gtggaggccg ccaacatcgt cagcgacgcc
     3901 aagctgggtc tgctcaccgc cgcgcgcacg cccgtgcggg agctcgaaca gcatctgggt
     3961 attcagctgc tgaaagagga cgaagaggcc gatatcgata cgctgggagg gctggtgttc
     4021 gcgctggttg gacgtgtccc cgcgcgcggc gagctgatcc ggcatccagc cggtgtcgag
     4081 ttcgaggtcc tcgatgccga tccgcggcgg gtcaaaaagc taagggtgca cgtcacgcgc
     4141 cagccagccg cggcggaagc cagcattgcc aaatcctgag ccgagccgcc gcgacgccgg
     4201 cgagcccgcg ccgcggggcc tcgcgcacat tctctgcaac ctgaccggct ggtcgcggcg
     4261 cctcagtcac ctcagcggct ggcggcggtt tgccgccgcc ttcgtcgccg gcctcgcctc
     4321 ggtgacggcg attgctccgt ttcatgcctg gcccgttctg tatctgacct tgcccgccct
     4381 gatctggctc ctcgatggcg cctgtgcccc ggcagccgcg ttcaagggtg cgcccaagcc
     4441 ggcggcggca tggcggggtg tgcgcgccgc cgcggcggtc ggctggtggt tcggctttgg
     4501 ctattttgtg gtggggctct actgggtcgg cgctgccttt ttggtcgaag ccgacgtctt
     4561 tgcgccgctg ttgcccctgg cggtgaccct actgccggca ggcctggcgc tgttctacgc
     4621 cggcgcggcc gcgctcgccc ggcccttttg gcgcaccgga cccacccgcg tcctcgcctt
     4681 ggcgctcgcg ctctcggcca tggaatatgc gcgcgggcat ctcttcaccg gctttccctg
     4741 gaacgtgctg ggctatgcgc tgacctaccc cttatccctg atgcaaagcg ccgccctggt
     4801 cggcatctac gggttgacgt tgctcggcgt tctgatctgg gcgggaccgc tggtgctctt
     4861 ggccgaagcc tcgcccggcc ggatgggacg agggcggcga cgctcggcgc tggcactcgc
     4921 cgttgcgccc gtcttggtgt tggcgatttg gggtcacctg cggctcgccg cggcccccgc
     4981 ggccatgctg cctggggtca agctgcgcat tgtgcagccg agcgtacccc aagcggagaa
     5041 gtggcggccc gacaaccagg agcgcatttt cctcgaccac ctacgcctct cggccagtac
     5101 tgcggcgggc gtccccgacg agcttgccgg catcacgcac gtgatctggc cagaggcggc
     5161 gatgcccttc ctccccgtgg atcacccggc cgcgcgcgcc gctatcgggc gtctcttgcc
     5221 gcccggcacg ctcctgattg ccgggggctt gcgcgccgag gccgaccccg aggcgccccg
     5281 tgggcggcgg atcttcaaca gcctgctggt gttcggtccc ggcggcagcc tgctcgcctc
     5341 ctacgacaag gcccacctcg tgccttttgg cgaatacctg ccgctcgcct cggtgctcga
     5401 ggcgcttggg ctcgagaccc tgacgcggcg gcgtggcggc tttgctcgcg gcccgacacc
     5461 ccgcccggtt ttgcacctgc ccggcctgcc cccggtgctg cccttgatct gctatgaggt
     5521 cattttcccg agcgccgtca acgctgggtc cgaacggccg gggctgctcg tcaatctcac
     5581 caacgacggc tggttcggca ataccaccgg cccgcgccaa cacatgcagc aggcgcgcgt
     5641 gcgcgcggtg gaagcaggcc tccccttgct tcgggccgcc aataacggca tctccggcct
     5701 gatcgacgcg cacggccgcg tcctcgtcga actgcgcctc gatcagcgcg gctcggccga
     5761 cacgggcctg cccgccgccc tgccgcccac cccctatgcc cgctttggcg atgccggttt
     5821 cgccggcctc tggctcctcg gggtgggact cttgggcctg gggcgccggc gccgccggcc
     5881 gccggcgtga gcctcgcagg caggcgccca tcgccttgca gtgcacctga ggttgacgcc
     5941 ttggtcatct gcacctacca attgcagagg caggacagca tggacggggc tgagtgaatg
     6001 tcgagaccaa gtgagttcgc cgcggaagcc gatggcgagg aaaaaggctc gcggcgaccc
     6061 aatcccattg acgtgcatgt gggcagccgg gtccgcttcc gccgcatgct gttgggcatg
     6121 agccaggaaa agctgggcga gaagctgggc ctgaccttcc agcaaatcca aaagtatgag
     6181 aagggcatca accgcatcgg cgccagtcgt ctgttcgatc tggcgcaggt cctcggcgtt
     6241 tccgtccagt tcttctacga ggaggcgccc accggggagg tgccgcgctt cgtcgccgac
     6301 ggttttgcgg aacggccggc cgaaagctcc atcgtcgagt tcttgcgcag ccgcgatggc
     6361 ctggagctga acaaggcgtt cgtccgtatc gccgatgtgc gggcccggcg tgccatcgtc
     6421 gagctcgtac ggagcttggc cgccgatgac gaccttctcg aatgagggcg ccggcctgcc
     6481 gggccaggcg cgccgacgag ctgccggtgc gccgtgcccg ggctcatctt gacccctccc
     6541 acaaacactg taacaagacg cgcaatttgg cgcctagcca tggggaacac cgtgtcgcga
     6601 aagtcttatt tgttcacgag tgagtcggtt tcggaggggc ctccggacaa ggtttgcgat
     6661 cgcatttccg acgaggtcgt cgaccttttt ttccgcgagg gcccgaaggc cggcgtcgat
     6721 ccttggcaac tcagggtcgc ctgcgaaacg cttgccacca ccaaccaggt ggtgatcgcg
     6781 ggtgaaacgc gcggacccaa gagcatcacc cgcgatctcg ttgcgcacgt ggcacgcatg
     6841 gccatcaagg acatcggcta cgagcaagag ggcttccatt gggaccgggc cgatattcgc
     6901 gtgctcttgc acccccagtc cgccgatatt gcgcaagggg tcgatagcgg cggcaaccgc
     6961 gacgagggtg caggcgatca gggcatcatg ttcggctttg cctgccgcga tacaccggaa
     7021 ctgatgccgg cgccgatcta ctacagccac aagatcctac gcctcatttc cgaggcccgg
     7081 cacgccggca aggaggcggt gctgggtccc gactccaaga gccaggtgac ggtgcgctac
     7141 gaaaacggca agcccgttgc ggcgacccag atcgtggtct cccaccagca cctggtggaa
     7201 gccatgacct cggcccaggt gcgcgaactc gtcgagccct acgtcaaaaa ggccctgccc
     7261 agcggctgga tcagcaacga cacgatctgg cacgtcaatc cgacgggcaa attccatatc
     7321 ggcggccccg acggcgactc gggcctgacg gggcgcaaga tcatcgttga tacctatggt
     7381 ggagcggccc cgcacggcgg gggagccttc tcgggcaagg atcccaccaa ggttgatcgc
     7441 tctgcggcct acgcggcgcg ctatctcgcc aaaaatgtcg tggccgccgg ccttgccgat
     7501 cagtgcacga tccagctgtc ctacgccatc ggcgtcgcca agccgctgtc gatttatgtc
     7561 gatgtaggcg ccaacccgag cggcatcgac acgcccaagc tcgagcgcgt gctgggcgag
     7621 ctcatggatt tgtcgccgcg cggtatccgc aaacacctcg agctcaacaa gccgatctac
     7681 gctcgcaccg ctgcctatgg gcactttggc cgcgagcccg ataatgaggg cggtttctcc
     7741 tgggagaaga ccgatctcgg tcccaagatc aaggcggcga tggcctgaac ttccacggcc
     7801 tgcccaaggc aaccgacgcc ggggtgccga gcggctgagc gatgggtctg ccatgctggc
     7861 gggcgcggac gagttgcgca gtttcgggcg ccggcgcggc cgcgcgctga gcccgcgcca
     7921 agccggcctc tggcgcgagc ttttgcccag ggttgccgtg ccgctgcagg gcgccgcgct
     7981 cgccaacccc ctcgacctct ttgccggcaa cgtgcgtgag gtgtggctcg aaatcggctt
     8041 tggcggcggc gagcatcttc tctggcaggc cgagcgccac cccgaggcgg gcatcctcgg
     8101 ctgcgagccc ttccaggatg gggtggtcaa ggtgttggcc gccctcgccg agcgcgacgg
     8161 cggcaatatt cgtgtgcatg ccgatgatgc ccgtcccctc atcaagcagc tgccggccgc
     8221 ctccgtcgcc cgcgtcttca tattgtttcc cgatccgtgg ccgaagcg
//
LOCUS       DASTMT010000066         3650 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_4615229, whole genome shotgun sequence.
ACCESSION   DASTMT010000066 DASTMT010000000
VERSION     DASTMT010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3650)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3650)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3650
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4615229"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(257..1237)
                     /locus_tag="VFR00_01405"
     CDS             complement(257..1237)
                     /locus_tag="VFR00_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EipA family protein"
                     /protein_id="HEU0157933.1"
                     /translation="MQSSIQRRPFGRQLTAGLLAVPLLLWATLGAAQETLPWGRGSQQ
                     PRYEDPAPPPRSNDAYRPPRADGYQGAYGQAPPAYGQAPPPYGQAPPAYGQAPPAYGQ
                     APPPYAGPPSQGYGQSYAGAPPQGYGQPYTPAPGGAGYDDPADRGYGQPPPYDRRYGG
                     YDDGPPGTYSSREILQTGHTFFGSVSKGLASVVEYAFKNQGRPNGYILGQEASGAFVA
                     GLRYGEGVLYTRDAGTHRVYWQGPSIGYDAGAEGSKVMMLVYNLRDPGDMYQRFGGVD
                     GSAYFVGGVGVTFQKSGDIVVAPIRSGLGLRLGANVGYLKYTPRPTWNPF"
     gene            complement(1368..2192)
                     /locus_tag="VFR00_01410"
     CDS             complement(1368..2192)
                     /locus_tag="VFR00_01410"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEU0157934.1"
                     /translation="MDAKDAKALAEGLLGVVIAAARVEMSHFGSGLVVERKADRSPVT
                     IADRAAEDIILRGLQRLAPGVPVVAEESVASGSLPLAADRFFLVDPLDGTKGFIRGEP
                     EFTINIALIERARPSFGLLYAPALADFYVTLGPSQAVNARLEPQADVRSLAGIDLKPI
                     HGRRADPSALVALTSQSHLNRATEAFLGRYDVSVQKRLASSLKFGWLAKGEADLYPRT
                     GETSEWDTAAGHALLSAAGGTVTTLEGEPLLYGKASNKFINTNFVAWGQLPLAPVR"
     gene            2234..3124
                     /locus_tag="VFR00_01415"
     CDS             2234..3124
                     /locus_tag="VFR00_01415"
                     /inference="COORDINATES: protein motif:HMM:NF021595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphotransferase family protein"
                     /protein_id="HEU0157935.1"
                     /translation="MDLVSLARRRKHTGEAIPARVDASVVTKKQYPGRRRVTALAGEA
                     QSMYHSNTCAQAVPEGQRPRARFVGPGRLICNETRGFAMAQGAEPTDLELAALISSKI
                     CHDVIGPVGAIYNGLEILDEDDDAEAKTYAMDVIRNVTEQASARLQFARFAFGAAGSA
                     GAQIDLATAEQISRGLLGKGKHRLSWRAKPGHMAKDKVKLLLNLIASAITALPRGGDI
                     EVLIQGTMESPSFQLRCRGTGVRPPQYLVDYVGGGQSPPLDAMSIQAYYTWRLAASSR
                     MRLAISREAGDILLLAKPLG"
     gene            3250..3615
                     /locus_tag="VFR00_01420"
     CDS             3250..3615
                     /locus_tag="VFR00_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU0157936.1"
                     /translation="MKTCLVVDDSDVVRKVARHIFHSLKFESREADSGPAALEACQSA
                     MPDAILLDWQMPAMGSVEFLTSLRAMPNGERPHVIYCATENSQRDIALALSSGADDYL
                     LKPFEREALRSKLAAAGLI"
BASE COUNT          637 a         1190 c         1232 g          591 t
ORIGIN      
        1 aggcggacgc cagcagcagt cccagcaggc tggccaccag gaagcctaaa gccaggagca
       61 tggcggattg gatggaaagc aagccctttt cctttgcccg gccgaatgtg tgcggcgacc
      121 acgactaact acgcacgacc cccctgccta gatccaagca tccgggcggc cgagggttgc
      181 ggaagccgcg ggcccgatac tagggggcga acgcctcgcc gtcacgccca ggctgccggc
      241 cggccgggcg gcacccctag aacgggttcc aagtcggccg gggcgtatat ttgaggtaac
      301 ccacattggc gccgagcctc aagccgaggc cggagcgtat cggagcaacg acgatatcgc
      361 cgctcttttg aaacgtgacg ccgacgccgc ccacgaagta ggccgagccg tccacgccgc
      421 cgaagcgctg gtacatgtcg ccaggatcgc gcaggttata gaccagcatc atcaccttgg
      481 agccttccgc gcccgcgtcg tagccgatcg aagggccctg ccagtaaacg cggtgcgtcc
      541 ccgcgtcgcg ggtgtagagg acaccttcac catagcgcag gcccgccaca aaggccccgc
      601 tcgcctcctg gcccaaaatg tagccgttgg gccggccctg gttcttgaag gcgtactcca
      661 ccacgctcgc caggcccttg ctgaccgagc cgaagaacgt atggccggtc tgcaggatct
      721 cgcgcgagga ataggtgccg ggcgggccgt catcgtagcc cccgtaacgc cggtcgtagg
      781 ggggaggttg gccataaccg cggtcggcag ggtcgtcata acccgcgcca ccgggggccg
      841 gcgtataggg ttgaccatag ccttgcggcg gggcgcccgc gtacgactgc ccgtagccct
      901 ggcttggtgg gccggcatag ggcggcgggg cctgcccata ggcgggcggg gcctgcccat
      961 aggcgggtgg ggcctgccca tagggcggtg gggcttgccc ataggcggga ggggcttgcc
     1021 cataggcccc ctgatagccg tccgcgcggg gtggccggta ggcgtcattg gatcggggcg
     1081 gcggggcggg atcctcatag cggggctgct ggctgccgcg cccccagggc aaagtctcct
     1141 gcgccgcgcc gagcgtcgcc cacaatagca gggggaccgc caacaggccg gcagtgagct
     1201 gacgcccgaa aggtcgtctt tgtatggatg attgcatggg ttcagtaccg cgaaagtccg
     1261 aacggccgca ttggccgggg caaagcccga agcaaagccc gcgctagctt aagaattgtc
     1321 gcaaagtgtt acgaaagtat ggccaagagg cggcccggcg ccgcccccta gcgcaccggc
     1381 gccagtggca gctgacccca ggccacgaag ttcgtgttga tgaatttgtt gctcgccttg
     1441 ccataaagca gcggctcgcc ctcgagcgtc gtcaccgtgc cgccggccgc gctcaacagg
     1501 gcatggcccg ccgccgtatc ccattcgctg gtctcgcccg tgcgcggata gagatcggcc
     1561 tcacccttgg ccaaccaacc gaatttcagc gaggaggcga gccgcttttg cacgctgacg
     1621 tcgtagcgcc caaggaaggc ctcggtggcg cggttgaggt gcgactggct ggtgagcgcg
     1681 acgagggcgc ttgggtcggc gcggcggccg tgaatgggct tcagatcgat gccggcgagc
     1741 gagcgcacgt cggcctgggg ctcgaggcgc gcattgacgg cttggctcgg tccgagcgtc
     1801 acgtagaaat cggcaagcgc gggcgcgtaa aggagcccga agctcggccg ggcccgctcg
     1861 atcaaggcaa tgttgatcgt aaactccggc tcgcccctga tgaagccctt ggtgccgtcc
     1921 agggggtcga ccaggaaaaa gcgatcggcc gcaagcggca agctgccgct cgccaccgat
     1981 tcctcggcga ccacgggcac ccccggcgcg aggcgctgca ggccgcgcag gataatgtct
     2041 tcggcggcac gatccgcgat cgtcaccggg cttcgatcgg ctttcctttc caccaccagc
     2101 ccgctgccga aatgggacat ctcaacgcgc gcggcggcaa tgacgacgcc gagcaggcct
     2161 tctgcgagcg ctttggcgtc cttggcatcc atgcccggtc tcgattggcc tcgtctggat
     2221 gggccttgcc ccgatggatc ttgtctcgct ggcgcgccgt cgcaagcaca ccggcgaggc
     2281 aatcccggcc cgggtcgatg cctcggtagt gacgaaaaaa caatacccgg gccgccggcg
     2341 cgtcactgcc ctggcaggtg aggcgcaaag catgtatcac agcaacacct gtgcgcaagc
     2401 ggttcccgaa ggccagcgtc cccgtgcgcg ttttgtcggg ccgggccgcc tcatctgcaa
     2461 cgagaccaga ggtttcgcaa tggcccaagg cgccgagccg actgatcttg agctggccgc
     2521 tttgatctcc agcaaaattt gccacgatgt gatcggtccg gtcggcgcca tatacaacgg
     2581 gttggaaatc ctcgacgagg acgatgacgc cgaggcgaaa acgtacgcca tggacgtcat
     2641 ccgcaatgtc accgagcagg catcggcgcg cttgcagttt gcccgcttcg ccttcggtgc
     2701 ggccggctcg gccggcgccc agatcgatct ggcgaccgcc gagcagatct cacgcgggct
     2761 gttgggcaag ggcaagcaca ggttgagctg gcgcgccaaa cccgggcaca tggcgaaaga
     2821 taaggtgaag ctgctgctca acttgatcgc cagcgccatc accgccctgc cgcggggcgg
     2881 cgatatcgag gtcctcatcc agggcacgat ggagtccccg agctttcagc tgcgctgtcg
     2941 cggcaccggg gtgagaccgc cgcaatatct ggtggactat gtcggtggtg gacagtcgcc
     3001 gccgctcgat gccatgtcga tccaagctta ttacacttgg cgcctggcgg cctcctcgcg
     3061 catgcgcctc gccattagcc gggaggccgg cgatatcctg ttgctcgcca agccgctggg
     3121 ttgaggcgac cggcggcaac gcaaagttaa tctcttggcg tcagggtcgc gcctggacgg
     3181 cgactgcacg gcgcgctgcg gcagcagtcg aacggcaggg accggttggc tttctcatcc
     3241 gagcgcttca tgaagacctg cctcgtggtc gacgattccg acgtcgtcag aaaagtcgcc
     3301 cggcacatct tccacagtct caagttcgag agccgcgaag ccgacagcgg cccggctgcg
     3361 ctcgaggcct gtcagagcgc catgcccgat gcgattttgc tcgattggca gatgcccgcc
     3421 atgggcagcg tcgagttcct tacgagcttg cgcgccatgc ccaacgggga aaggccgcac
     3481 gtgatctatt gcgccacgga gaatagtcag cgcgatatcg ccctggcgct gtcgagcggc
     3541 gcggacgact atcttttgaa gccgttcgag cgcgaggcgc ttcggtccaa gctggccgcc
     3601 gccggcctga tctaggggaa aggcgccaac gccgccggcg gcgtgcgcgt
//
LOCUS       DASTMT010000067         3258 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_1352003, whole genome shotgun sequence.
ACCESSION   DASTMT010000067 DASTMT010000000
VERSION     DASTMT010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3258
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1352003"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..707)
                     /locus_tag="VFR00_01425"
     CDS             complement(<1..707)
                     /locus_tag="VFR00_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002715222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0157937.1"
                     /translation="MAQAVVGRLPEDEHRIQLRRAVVASTIGTAIEWYDFFLYSTVTG
                     LVFAKLFFPHSDPLVGTLEAFAVYAVGFLARPVGAAIFGHYGDRIGRKATLIATLMLM
                     GIATFLVAFVPTYESIGIWGAVILTVLRFVQGVGVGGEWGGSVLLSMEWSRTHAQRGF
                     IASWPQFGVPCGLFLANLAVLAFSQMSGEEFLAWGWRLPFALSIVLVGVGLYIRLGIL
                     ETPTFTALQEAERLDRAP"
     gene            complement(868..1125)
                     /locus_tag="VFR00_01430"
     CDS             complement(868..1125)
                     /locus_tag="VFR00_01430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0157938.1"
                     /translation="MKARQLVDSGSFGPEKLKVARQAFDDAWSSVAGHFGTDPGVIEA
                     ARLKLANAILAMCHEEMGDPVALKKAALGVLAREFRLDLPL"
     gene            complement(1223..1948)
                     /locus_tag="VFR00_01435"
     CDS             complement(1223..1948)
                     /locus_tag="VFR00_01435"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEU0157939.1"
                     /translation="MTPPQNWPRNRLLLALPEADLKQLQPDLEFIECKREQVLVDADS
                     SLDHIYFPDSGVISVVAVYPDGSIIEMATIGREGSTGFQAVFGAKASSARLLVQVPGT
                     AAKMTRQAFAKAMDAMPTFLKLVQAHVLAFLEQVMVSAACNGAHNVRQRLARWLLMMR
                     DRHDSDNLPLTQDLLAEMLGVHRPSITNAAQGLQRDGLIECGRRRVTILDRDGLIGAS
                     CECYRLVRTRIAYHLPKTFPEAL"
     gene            complement(2041..>3258)
                     /gene="trmFO"
                     /locus_tag="VFR00_01440"
     CDS             complement(2041..>3258)
                     /gene="trmFO"
                     /locus_tag="VFR00_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012332354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HEU0157940.1"
                     /translation="TNAVGLLHEEMRRAGSLIMTAGDTHKLPAGGALAVDRDEFARAV
                     TDALSSHPLIGIERTEVAGLPPPAWESVIIATGPLTSPALSAAIQELTGEGELAFFDA
                     IAPIVHLDSIDPQITWRQSRYDKAGPGGTGADYINCPMSAAEYEAFVAALIAAEKTSF
                     QAWEASTPYFEGCLPIEVMAARGRETLRFGPMKPVGLRNPHRSGERPYAVVQLRQDNR
                     LGTLWNMVGFQTKLKHGEQVRVFRTIPGLAQAEFARLGGLHRNTFLNAPKLLDSTLRL
                     RAAPRLRFAGQITGCEGYVESAAIGLLAGQAAAAERRGLAWGPPPATTAFGALLGHIT
                     GGHLGAEADGAGAFQPMNINFGLFPPLPAAGVGGARAKGKERGAAKKRALSTRALSDL
                     DAWLARPHVNAAE"
BASE COUNT          601 a         1076 c         1088 g          493 t
ORIGIN      
        1 ggcgcgcggt ccaggcgctc ggcctcttgc agcgccgtga aggtcggcgt ctccagaatg
       61 cccaagcgga tataaagacc gacgccgacc agcacgatgc tgagcgcgaa gggcaggcgc
      121 caaccccagg ccaaaaactc ctcgcccgac atttggctga aggccagcac cgccaagttg
      181 gccaaaaata gtccgcacgg caccccgaat tgcggccagg aggcaataaa gccgcgctgc
      241 gcgtgcgtgc gcgaccattc catggaaagc agcaccgagc cgccccactc gccaccgacg
      301 ccgacgcctt gcacaaagcg cagcacggtc aagatcacgg cgccccaaat accgatgctc
      361 tcgtaggtcg gcacgaaggc gacgaggaag gtggcgatcc ccatcagcat cagcgtcgca
      421 atcaacgtgg ccttgcggcc gatgcggtcc ccgtagtggc caaagatggc ggcccccacg
      481 ggccgggcca ggaagcccac ggcatagacg gcaaaggcct cgagcgttcc caccagggga
      541 tcggaatggg gaaagaacag cttggcaaag accagaccgg tgacggtgct gtagagaaag
      601 aagtcatacc actcgatggc cgtaccgatg gtgctggcga ccacggcgcg gcgcaactga
      661 atgcgatgct cgtcttcagg aaggcggcca acgacggctt gggccatggc ataactccgc
      721 gctgccccgc cagctgccga cccctttaac gtgccaacgc gcgccaaagt tccggccccg
      781 gacggctgcc atgcacgggc acagcctgaa ccgctgcgtg ccggtacgtc ggccgccggg
      841 gcgccgagag cgaccgacgg ggcggcgcta gagcggcagg tccagccgga attcgcgtgc
      901 cagcacgccc aaggccgcct tcttcagggc cacggggtcg cccatctcct catggcacat
      961 ggcgagaatg gcgttggcaa gcttgaggcg tgcggcttcg atgactccag gatcggtgcc
     1021 aaagtggccg gcgacgctgc tccaggcatc atcaaaggct tgacgggcga ccttaagctt
     1081 ctcgggccca aaggacccgc tatcgacgag ctgacgtgcc ttcatgaaat ccctcagggc
     1141 gaaaaaaacc gcccccgcac aggcggcgca atccctaaga accatggccg gggggtccga
     1201 ccctgtcggt aagctaggtg acctaaaggg cttcgggaaa cgttttcggc aggtgataag
     1261 caatgcgcgt gcgcaccagc cgatagcact cgcaggaggc cccgatgagg ccgtcgcggt
     1321 cgaggatggt gacccggcgc cggccgcact cgatcaagcc atcgcgctgc agcccctggg
     1381 cggcattggt gatgctcggt cggtgcacgc ccaacatttc cgcgagcaga tcctgggtca
     1441 agggcaggtt gtcgctgtcg tgacggtcgc gcatcatcaa gagccagcgc gccaagcgct
     1501 gacgcacgtt gtgggcgccg ttgcaggcag ccgacaccat tacctgctct aaaaaggcca
     1561 gcacgtgcgc ctgcaccagc ttgagaaagg tgggcatcgc gtccatggct ttggcgaacg
     1621 cctggcgcgt cattttggcc gccgtgcccg gtacctgcac cagcaagcgg gccgaggagg
     1681 ccttggcacc gaacaccgcc tggaaccccg tgctgccctc gcgtccgatg gtggccatct
     1741 cgatgatgct gccgtcggga tagaccgcga ccacggaaat caccccgctg tcggggaagt
     1801 agatgtgatc gagcgagcta tccgcatcaa ccaacacctg ctcgcgcttg cactcgatga
     1861 actcgagatc gggctgcagc tgcttgaggt cggcttcggg aagggcaagc aggaggcgat
     1921 tgcgcggcca gttttgcgga ggagtcatgc aacgtaaacg gcagaccgcc ggtctggttc
     1981 ggcccgggag cgcctttcgt ttcgcacccg cactacgggc cccgccgcgc cggcgagcgg
     2041 ctattcggcg gcgttgacgt gcggccgagc cagccaggca tcgaggtcgg atagagcgcg
     2101 cgtgctgagt gcgcgctttt tcgccgcgcc gcgctccttg cccttggcac gcgctccgcc
     2161 cacgcccgct gccggcagcg gcgggaacag tccaaaattg atgttcatgg gttggaaggc
     2221 gccggcacca tccgcctcgg cgcccaggtg cccgccggtg atgtggccga gcagcgcgcc
     2281 gaaggctgtc gttgccggcg gcgggcccca ggcgaggccg cgacgttcgg ccgccgcggc
     2341 ctgtccggcc agcagcccga tggcggcgct ttccacataa ccctcgcagc ccgtgatctg
     2401 acccgcaaac ctcagccgcg gggcggcgcg caagcgcaac gtgctatcga ggagcttggg
     2461 ggcattcaag aaggtgttgc ggtgcaagcc gccaaggcgc gcaaactccg cctgggcgag
     2521 gcccggaatt gtgcggaaga cccgcacctg ctcgccatgc ttgagcttgg tctggaagcc
     2581 gaccatgttc cacagcgtgc ccagtcgatt gtcttggcgc agctgcacga cggcgtaggg
     2641 acgttcgccg ctgcgatggg gattgcgcag gccaacgggc ttcatgggac caaagcgcag
     2701 cgtctcgcga ccgcgcgccg ccatcacttc gatcggcagg cagccctcga agtagggcgt
     2761 ggaggcctcc caggcctgaa agctcgtctt ctcggcggcg atcagcgcgg cgacgaaggc
     2821 ctcgtactcg gccgcgctca tcggacaatt gatgtaatcg gcgccggtgc cgccgggacc
     2881 ggccttgtca tagcgcgatt gacgccaggt gatctgcgga tcgatgctgt cgagatgaac
     2941 gatcggggcg atggcatcga aaaaggccaa ctcgccctca cctgtcagct cttggatggc
     3001 cgcggagagg gccggcgagg tgaggggtcc ggtggcgatg atgacgctct cccacgcggg
     3061 tggcggcaga ccggcgacct cggtccgctc gatcccaatc agcggatggc tgctgagcgc
     3121 atcggtcacg gcccttgcga actcgtcgcg atcgacggcc agcgccccgc ccgcaggcag
     3181 cttgtgggta tcgcccgcgg tcatgatcag ggagcccgcg cgccgcatct cctcgtgcag
     3241 cagcccgacg gcgttcgt
//
LOCUS       DASTMT010000068         3193 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_2993670, whole genome shotgun sequence.
ACCESSION   DASTMT010000068 DASTMT010000000
VERSION     DASTMT010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3193
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2993670"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..206)
                     /locus_tag="VFR00_01445"
     CDS             complement(<1..206)
                     /locus_tag="VFR00_01445"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEU0157941.1"
                     /translation="MFEVPLLIGNDDRNAAGGASFDRLNPLTGAVATRAAAASLNDVA
                     AAAAAAAAAFPAWSETGPAARRALL"
     gene            complement(339..1055)
                     /locus_tag="VFR00_01450"
                     /pseudo
     CDS             complement(339..1055)
                     /locus_tag="VFR00_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020009813.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(1428..2765)
                     /locus_tag="VFR00_01455"
     CDS             complement(1428..2765)
                     /locus_tag="VFR00_01455"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEU0157942.1"
                     /translation="MSAINRLLLLVGAVAATAGCAASLDAGQARLCRAVIPALNAGAL
                     GIEVARTGPLPTGDGVRVDYRAQSAAGQRSRFLECRFAGAREGGQLAAVSTEAGPLGD
                     LRLYVLKRFWLGPEGILADPEPISHAAEAPVLPRGLAIGLQHVIFALPPIAIYALLAA
                     AYSLLYGLIGRINLAFGELAAVAGYAALLGIALWGPPPSQAVLLFSVLLALLAATLHG
                     VVVGRLLFAPLLRSAGQHVLIGSIALAIVLQEYLRLTQGAKLIWVPPVFNTPYALARS
                     GDFVVTFTPMAMAAAGVCGLAALALLVLLRSTRFGRAWRACADDAGAAALFGVDHRLL
                     LLKTFALASALAGLAGYVVTVYYGAFGYAGGIVLGLKSLLAAVAGGIGRVGGAFLGGV
                     LIGAIEQVWSALFPIEFRDLAVFILLVVLLVLRPSGLFGWREPLGQKPLPGLG"
     gene            2905..>3193
                     /locus_tag="VFR00_01460"
     CDS             2905..>3193
                     /locus_tag="VFR00_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0157943.1"
                     /translation="MRLPFFYGWLIVAVVFVSMGIGVNVRTAFSLLFPPILGEFGWER
                     GVTAGAFSFGFVVSAVFSPLIGRLMDRAGPCLVMEAGVVLMGAGLLLAPLTQ"
BASE COUNT          588 a         1104 c         1052 g          449 t
ORIGIN      
        1 agcaaggcgc gacgtgccgc ggggcccgtc tctgaccagg cggggaaggc ggcggcggcg
       61 gcagcggcgg cggcggcaac gtcgttgagc gaggcggcgg cagcgcgcgt ggcaaccgcg
      121 ccggtcaggg gattgagccg atcgaaactg gccccgccgg cagcgttgcg gtcgtcattg
      181 ccgatcaaca acggcacctc gaacatgcgc tctctcccga agtgtggggc cgtggtagac
      241 aagactgctg gggcagaatg gacactgcgg ccgatctcgg ccgaaccgtg gcccgccgac
      301 gcggacgtag cggtcgggcc gttgtccgag tggggaccgg cagcgagggt gccgccgaga
      361 tagacctgct ccacggccgg ctcgctcttg agatgggcag cgtcgccctc gccgacgatg
      421 cggccgttct cgagaagata gcctcgatcg gcgatcgcca ggctctggcg cgcgttctgc
      481 tcgaccaaca gcaccccgac gccgagcccg cgaatgcgcg cgagcgcggc aaacagttcc
      541 ttgcaaagaa gcggcgccaa gccaagcgag ggctcatcga gcaacagcac gcagggatcc
      601 gacatcaggg cgcgaccgat cgccaccatc tgctgctcgc cgccgctcat cgtgcgcaca
      661 gtctggccct gacgttcggc gagccgcgga aagagcgcca gcactttggc taggctcgcc
      721 gcctgcttgg gccgcgcacg gcggctatag ccacccagca gcaaattctc gcgcacatcg
      781 aggtcggcaa aaatgccgcg gtcctcgggc acgagcacca ggcctcgctc gacgatgtcg
      841 tgcgctggca gacgcgcgag ctccgcccca tcgagcacga tgcgggcgcc ggcggcgacg
      901 gctacgagcc cgcccaaggc cttgagacag gaggatttgc cggccccatt ggcccccaac
      961 agcaccacaa tctcgccacg gcgcacgcag aaggcggcgt ccgccagggc gaggtgcttg
     1021 ccgtagcgta ccgacagccc ggcaacctca agcatacgtc ccttccccgg ttcgcccgcc
     1081 tcgccgcagc agagctcgcg cacgggccgc ccccatctaa aggccgggca ccgccgcagg
     1141 gtcaaccccg ttcgtggtca ttacctaatg gccagctgcg ccgcgcggcg ggcagcacgg
     1201 cggcagggtg gtcggtccat taggtaatcg gcccgtgcgt ttctagggga gttacccggt
     1261 ttcgccaaaa gcgccacctc gctaggttgg cattgctctc tggggctgcc tgaaccctcg
     1321 agagtgcttc ccacagcagt agttggcctg gctctgatct gccctccccc ctaaacagat
     1381 cagagccatt ttcttttgcc cgcgcgcaaa gcgcccgcgt gcacccctca gcccagcccc
     1441 ggcagcggtt tttgtcccaa cggttcgcgc cagccgaaca ggccgctggg gcgcagcacc
     1501 aacagcacaa cgagcaaaat aaacacagca aggtcacgaa actcgatcgg aaagagcgcc
     1561 gaccagacct gctcgatggc gccgatcaat acgccgccca gaaaggctcc gccgacgcgg
     1621 ccgatcccac ccgccaccgc cgccaacagc gacttgaggc cgagcacgat accgccagcg
     1681 taaccaaacg ccccgtaata gacagtgacg acataacctg ccaagccggc gagcgcagag
     1741 gcgagcgcaa aggtcttgag caacagcagt cgatggtcga cgccgaatag ggcggcggcc
     1801 ccggcatcat cggcgcaggc gcgccaggcg cggccaaacc gcgtcgacct caacagcacc
     1861 agcaaggcca acgcagcgag gccacacacc cccgcggcgg ccattgccat cggcgtgaag
     1921 gtgacgacga agtcgccgga acgcgccagc gcgtaaggcg tgttgaacac gggcggtacc
     1981 caaatcagct tggcaccctg ggtgaggcgc agatactcct gcagcacgat ggcaagcgcg
     2041 atactgccga tcaacacgtg ctggcccgcc gaacgcagca agggagcgaa gagcaggcgt
     2101 cccacgacaa ccccgtgcag cgtggcggcc agcagcgcca agagcacgct gaagagcagc
     2161 acggcctggc tcggcggtgg accccagagg gcaattccca gaagcgcggc gtaacccgcc
     2221 acggccgcga gttcgccaaa ggcgagattg atgcgtccga tgagcccata aaggagcgaa
     2281 taagccgccg cgagcagggc gtagatggcg atgggcggca aggcaaaaat cacgtgctgc
     2341 aagccgatgg caagcccacg cggcagcacg ggagcctccg ccgcgtgact gatgggctca
     2401 ggatcggcca gtatgccctc tgggcccaac caaaagcgct tgagcacata aaggcgcaga
     2461 tcgccgaggg ggcccgcttc cgtgctgacc gccgcgagtt gccccccttc acgtgcgccg
     2521 gcaaaccggc attccaggaa ccggctacgc tgacccgccg ccgactgggc acgatagtcg
     2581 acgcgcacac cgtcaccggt cggcagcggg cccgtgcgcg cgacctcgat gccgagcgcc
     2641 ccagcgttga gtgcggggat caccgcccgg cacaggcggg cctggcccgc atcgaggctg
     2701 gccgcgcagc cggccgttgc ggccacggcc ccaaccagca gcagcaggcg gttgatcgcg
     2761 gacatgcgca aaccctagct ccgccccgca atttggcaat gtctggcgga ggcgaccacg
     2821 gccgctggcc ggcgacagcc tcgccgggac gtgctaaggc gcgctttcgc ttcgccgggc
     2881 gccgcccctt gcctgatcac gtccatgcga cttcccttct tctatggttg gctgatcgtt
     2941 gctgtcgtgt tcgtcagcat gggcatcggc gtcaacgtcc gcaccgcctt ctctttgctg
     3001 tttccgccca tcctcggcga gtttggttgg gagcgcggcg tgacggcggg tgccttttcc
     3061 ttcggctttg tggtttcggc ggtattcagt ccactgatcg gtcggttgat ggatcgggcc
     3121 ggtccgtgcc tggtgatgga agcgggtgtc gtgctgatgg gggcggggct gctgctggcg
     3181 cccctgaccc aag
//
LOCUS       DASTMT010000069         3012 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_3321628, whole genome shotgun sequence.
ACCESSION   DASTMT010000069 DASTMT010000000
VERSION     DASTMT010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3012)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3012)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3012
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3321628"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..769
                     /locus_tag="VFR00_01465"
     CDS             <1..769
                     /locus_tag="VFR00_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016843589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018784 - (S)-2-haloacid dehalogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="haloacid dehalogenase type II"
                     /protein_id="HEU0157944.1"
                     /translation="RAVRGDRRGLAAARLKARQGVGSNGLLKREIKALAFDQYGTIVD
                     MQKGLTEAARPFLAAKGWSGKPDSFVTWWRRTHFESSMIDALCDRGHTPYREIGHRAV
                     SYVMDRCGLSYSQEEVCWLVSQIESLRPFPDVIAALEKLRAANYKLAILSNGDPDMLQ
                     AAGPHIGFRFDHVISVAEAGYFKPHWKTYAKAEELIGKDRSSILFVANHAFDCIGAKS
                     FGMRSAFIDRRRRPFGETPHQPDLIVANFAELAAALA"
     gene            complement(791..1183)
                     /locus_tag="VFR00_01470"
     CDS             complement(791..1183)
                     /locus_tag="VFR00_01470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0157945.1"
                     /translation="MQLAPMRYVKIDGTAVAVTVASASEAKAALKELRHKKREIKFLR
                     GALLRRQKAARPEPSKRRRSVSFFTRLWRAMRWLSAALLDLATVSARERKQRSPAEIE
                     RELRWTDEVLHNIEGCILQLEGKLLTHT"
     gene            1591..2142
                     /locus_tag="VFR00_01475"
     CDS             1591..2142
                     /locus_tag="VFR00_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013949509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase family
                     metallohydrolase"
                     /protein_id="HEU0157946.1"
                     /translation="MHKRLCIVALGLSLGLITTAASSRTYNFEQNTWAADDGDRSSAS
                     AHRHRVAYAYRSDGEEAEPLNRRSVTREHGGMRQGGGATSRSCLKASARALLDRIESH
                     FGKMQIISTCRPGAVIAGTGRRSKHASGEAIDFNAGGRKGEVVRWLIANHKSGGTMTY
                     RGMSHIHVDVGYHFVSLNSSSGR"
     gene            complement(2202..2951)
                     /locus_tag="VFR00_01480"
     CDS             complement(2202..2951)
                     /locus_tag="VFR00_01480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0157947.1"
                     /translation="MLAPLVLVVVAVAVVVTSFISGVFGMAGGMLLLGLLLLFMDVGP
                     AMVLFGVIQLAANGWRSVLWWRHVAWNIVWRFLVGSTAMFFVMRTIAIFPGKAAIYLG
                     LGLIPFAAYALPRRLSPDITRPGVPYLCGAAIMVLQLLAGAAGHVLDIFFQKSPLDRR
                     TIVGTKAVTQVTGHVYRILYFGSFEVTFDAQVPAWGYVGAMVLACAGTSLAGLVLLRM
                     TEAGFRAWSRRITIGVALTYLVRGVWLLALP"
BASE COUNT          579 a          992 c          977 g          464 t
ORIGIN      
        1 gcgggctgtt cgtggcgatc ggcgcggcct tgccgctgcg cggctgaagg cgcgacaggg
       61 cgtgggcagc aacggcctcc tcaagcgcga gatcaaggcg cttgcgttcg accagtacgg
      121 caccatcgtc gacatgcaga agggcctcac cgaggcggcg aggccgtttc tcgccgccaa
      181 gggctggagc ggcaagcccg acagcttcgt cacctggtgg cggcgcaccc atttcgagag
      241 ctcgatgatc gacgcgctgt gcgaccgcgg gcacacgccg taccgggaga tcggccaccg
      301 cgccgtgtcc tacgtgatgg atcgctgcgg cttgagctac agccaagagg aggtgtgctg
      361 gttggtcagc cagatcgaga gcttgcggcc gtttccggac gtgattgccg cgctcgaaaa
      421 gctgcgcgcg gccaactaca aattggcgat cctctcgaac ggcgaccccg acatgttgca
      481 ggctgccggt ccgcacatcg gctttcgctt cgaccatgtg atctcggtgg cagaggctgg
      541 ctacttcaag ccgcactgga aaacctacgc caaggccgag gagctgatcg gcaaggaccg
      601 ctcgagtatt ctcttcgtcg ccaaccacgc cttcgactgc atcggtgcca aatcgtttgg
      661 catgcgcagc gctttcatcg accggcgccg acggccgttc ggagagacgc cgcaccagcc
      721 cgatttgatt gtggcgaact tcgccgaatt ggcggcagcg ttggcttgag cctcgcgggc
      781 cgcggcaggc tcaggtgtgc gtcaagagct tgccctcgag ctgcaggatg cagccctcga
      841 tattgtgcag cacttcatcg gtccagcgca gctcgcgttc gatttcggcg gggctgcgct
      901 gcttgcgctc gcgcgccgac accgtggcaa gatcgagcag cgcggcgctg agccaacgca
      961 ttgcgcgcca caggcgcgtg aaaaaggaga ccgatcggcg gcgtttgctg ggctccgggc
     1021 gcgcggcctt ttgtcgccgc agcagcgctc cgcgcaagaa tttgatctcc cgctttttgt
     1081 gccgcaactc cttcagggcc gccttggctt cgctcgccga cgccacggtc acggcgaccg
     1141 ccgtgccgtc aatttttacg taacgcattg gcgcaagttg catcgccgcc cctcgctcga
     1201 tggctgatgt ccgacacagt acacgccgat ggggcagccg tcaggcctca ccaccggcgg
     1261 cagttgctgg cgcgcgttta aggcggctct gcacgcgatg cgcgaagggg ctgtcgcgac
     1321 ttgggaaagg cgccgcaatc tcgccatagg ccgtcgtcca aggtgcccgc gtcagggagc
     1381 aacgctcccg catcggccgc caggcgactg acggcccaac ggagggagac aaaagtacgg
     1441 gtgagggatt cggtccggtc gacgcactag ggatgcgccc ggcaagacgg ggactaccag
     1501 cgccatcgaa gccgaatgag ccgcgggctc gcgtgcgtac gcgcgcgccc tcggcaacct
     1561 caagtgacaa tcgcaaaagt aggagttccc atgcacaagc ggctttgcat cgtcgcgctc
     1621 ggcctatcgc tcggcctcat cacaacggca gctagctcac gcacctacaa tttcgagcaa
     1681 aatacctggg ctgccgacga cggcgatcgc tcttctgctt cggctcatcg gcaccgtgtc
     1741 gcttacgcct accggtccga tggcgaggaa gccgaacccc tcaatcggcg cagcgtaacg
     1801 cgcgagcacg gcggcatgcg ccagggcggc ggcgccacct cgcgcagttg cctgaaggct
     1861 tccgcccgcg ccctgctcga tcgcatcgag agccacttcg gcaaaatgca gatcatctcg
     1921 acctgccgtc ccggcgcggt cattgccggc accggccggc gctcgaagca tgccagcggt
     1981 gaagccatcg atttcaacgc cggcggccgc aagggcgagg tggtccgctg gctgatcgcc
     2041 aaccacaaat ccggtggcac catgacctat cgcggcatga gccatatcca cgtcgatgtg
     2101 ggctaccact tcgtctcgct caactcgagc agcggcaggt agacgcccaa gcgacaaaca
     2161 cgggggcgcg cgctgtgggc cgcgccctcg tgcgtaaaag ttcagggaag cgcgagtagc
     2221 caaacaccgc gcaccagata agtgagcgcg acgccgatgg tgatgcgtcg gctccaggcc
     2281 cggaagccgg cttcggtcat gcgcagcaag acgagccccg ccaacgaggt gcccgcgcag
     2341 gcgagcacca tggcgccgac gtagccccag gcgggaacct gggcgtcgaa ggtgacctcg
     2401 aaggagccga agtagaggat gcggtagacg tgtccggtga cctgggtcac cgccttggtg
     2461 ccgacgatcg tgcgccgatc gagcgggctc ttttggaaga aaatatcgag cacgtgtccg
     2521 gcggcgcccg ccaaaagctg caggaccatg atggcggcgc cacacaggta gggcacgccc
     2581 ggtcgcgtaa tgtcgggcga cagccgccgt ggcaaagcgt aggcggcgaa ggggatcaga
     2641 cccagcccga gatagatggc cgccttgccg ggaaaaatag caatcgtgcg catgacgaag
     2701 aacatcgccg tcgaccccac caggaagcgc cacacgatgt tccaggcgac gtggcgccac
     2761 cagaggaccg agcgccagcc attggccgcc aactggatga caccgaagag caccatggcc
     2821 gggcccacgt ccatgaacag caacagcagg ccaagcagca gcatgccccc ggccatcccg
     2881 aaaaccccgg aaatgaaaga ggtgacgacg acggcgaccg caaccaccac cagcacgagg
     2941 ggagcaagca tttaactttt accgcgccgc atcgggcctg acctcttgcc gacgccctta
     3001 gacggccgca gg
//
LOCUS       DASTMT010000070         4750 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_2009557, whole genome shotgun sequence.
ACCESSION   DASTMT010000070 DASTMT010000000
VERSION     DASTMT010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4750
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2009557"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(535..1011)
                     /locus_tag="VFR00_01485"
     CDS             complement(535..1011)
                     /locus_tag="VFR00_01485"
                     /inference="COORDINATES: protein motif:HMM:NF039485.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HEU0157948.1"
                     /translation="MGMLDEVIAAALGARGQPSQGQPAQGRPAPDQFSQIAAALTALL
                     APKPGPAAAGRAAAPASQAAPTGLDGLVDQFKQSGLEDVINSWIGTGQNQSISPNELR
                     EALGQKTVTDLSRQSGAPQDDLLSQLSKYLPTVIDKLTPNGQLPNQADLGPGYRSR"
     gene            complement(1201..1617)
                     /locus_tag="VFR00_01490"
     CDS             complement(1201..1617)
                     /locus_tag="VFR00_01490"
                     /inference="COORDINATES: protein motif:HMM:TIGR03668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03668 family PPOX class F420-dependent
                     oxidoreductase"
                     /protein_id="HEU0157949.1"
                     /translation="MRERRIAHLATADRAGVPHVVPVCFVVSGRTLYVTIDRKPKRQP
                     PRLLKRLRNIAENAQVAVVVDRYEEDWGRLGWVMLTGCADILAQGEEHDAAQDLLRGR
                     YPQLAAMQIAELPVIAVRIARVTSWGNLAFDGGNTS"
     gene            complement(1703..2509)
                     /locus_tag="VFR00_01495"
     CDS             complement(1703..2509)
                     /locus_tag="VFR00_01495"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEU0157950.1"
                     /translation="MGRAHGLGRRHWHGMPLITMAGIQLVAMGAAVSAATAALSLPLE
                     LGWRGWVGAFSCYGVVTTFVLLDLARHAPHQRFGTANSVTLGRAGLTALLWGIVGEAL
                     PGPLALDAELGWLLAGAATTALLLDGVDGWIARRSGMVSQFGADFDLEVDCLFVLALS
                     LLVYAAGRTGAWVLSCGLMRYLFVGAGWLWPTLAAPLQPLRRRKVIAAIQGGVLIATL
                     VPVLPASAAPPLCLGALALLIYSFGADLVWLTGQGGEAPPELRSRGASLG"
     gene            complement(2718..3053)
                     /locus_tag="VFR00_01500"
     CDS             complement(2718..3053)
                     /locus_tag="VFR00_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006019368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYD domain-containing protein"
                     /protein_id="HEU0157951.1"
                     /translation="MPIYISRGKFTTDAVKGMLAKPENREDAVGTLFKSVGGKLLGWY
                     LTFGRHDWLVIGEFPNEKAAASAILAAAAGGSLSDIETTVAMTAKEAAATFEAAGTVA
                     KGFRSAGRT"
     gene            3592..3798
                     /locus_tag="VFR00_01505"
     CDS             3592..3798
                     /locus_tag="VFR00_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018641487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase, NapE protein"
                     /protein_id="HEU0157952.1"
                     /translation="MTSSRASTPPSRRKQELIVFVLLSLVLAPVLAVATVGGYGLGIW
                     VYQMWRGPPGPPPKAPRSPGVVPQ"
     gene            3819..4127
                     /locus_tag="VFR00_01510"
     CDS             3819..4127
                     /locus_tag="VFR00_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006614931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HEU0157953.1"
                     /translation="MRLTRRALISGQAEPGPAPAGVEIVSLLVSARPQFMPAVAEAIL
                     ALGNCEIHARDPRGKLIVVLEEESTGAVGAKINTISGLPHVLSAVMVFQATDADATIG
                     "
     gene            4136..>4750
                     /locus_tag="VFR00_01515"
     CDS             4136..>4750
                     /locus_tag="VFR00_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012029476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEU0157954.1"
                     /translation="MTDAALNRRQLLKTQAAMAAAAVAGLPTSSVAQLITDRKESELA
                     WNKAPCRFCGTGCGVLVATKDGRVVATHGDAKAEVNRGLNCVKGYFLSKIMYGEDRLR
                     EPLLRKKNGKYDKSGEFTAVSWDEAFTVMAEQFRAALKKGGPSAVGMFGSGQWTIWEG
                     YAAAKLFKAGFRSNNLDPNARHCMASAAAGFMRSFGIDEPMGCYD"
BASE COUNT          868 a         1602 c         1489 g          791 t
ORIGIN      
        1 acccagcttc tcgagcccgc cgccaatcaa ggcgataccg ctgggcggat tcttgagcgg
       61 gccgaccgcc gcattgcagg cttggttgcc gatcaatagc gccagccgcg tctgcgcctg
      121 ttgtgccgat acgccgcagc agcagaggac gaaggcgacc agcgcagata ggcttagcca
      181 accgcaccgc gtgtctcgtc accacgcgcg ctgtgctcac agctctcaac gcctcggcgc
      241 cgcgagagtt ttccccatgc ctgcgtcgcg cgctcgaagg acacggacga ggctgcggcc
      301 agcgcgctgt tgccaatcga ttgggcattt tcgctggcgc ggcttcgcgt atcctctagt
      361 cgccgggcgc ggcaaggcca aacactcaga gggcccgcgc ctgacgcacg ggcctctcgc
      421 aatcgctgag gggatgaggg ctccggcggt gctgcgtcca cgcaggtgtt ggcagcggcc
      481 ctggcgggcc agcagggacc acgcgggctg cgacctcgcc ggtgcgctcg ccgactaccg
      541 gctgcgatat cccgggccca ggtccgcctg gttcggaagc tggccgttcg gcgtcaactt
      601 atcgatcacg gtgggcagat acttggagag ctgcgaaagc aggtcatcct gcggtgcgcc
      661 cgactggcgc gaaagatcgg tgacggtctt ctgtccgagc gcctcgcgca gttcgttggg
      721 agaaatggat tggttctggc cggtgccaat ccaggagttg atcacgtcct caagcccgct
      781 ttgtttgaac tgatcgacga gaccgtcaag accggtgggc gcggcttgcg aggcaggggc
      841 ggccgcgcgt ccggctgcgg cgggccccgg tttgggcgcc aacagcgccg tcaatgccgc
      901 ggcaatttgt gagaattgat ccggcgcagg tcgaccttgc gcgggttgac cttgcgaggg
      961 ctgacccctg gcgccaagcg ccgccgcgat gacctcatcg agcattccca tgtgagttcc
     1021 tcccagaagc gacgccaaaa cggcggcagg cgcgattgtg cgcgcacatg cgccaacgaa
     1081 aatcggcaca aaccctgaat acgccccctg cattcgcttg ctttttgcgg aattcgcagc
     1141 tcttggctgg ccgcacaacg catcgcgcag caagctccga ggcctgccgc gtccttgcgc
     1201 ctaggacgtg ttgcccccgt cgaatgcgag gttgccccaa ctcgtcacgc gcgcgatgcg
     1261 aacggcaatc acgggaagct cggcgatctg catcgccgcg agctgcggat agcgaccgcg
     1321 caggaggtcc tgcgccgcat cgtgctcctc gccctgcgcc aggatatcgg cgcagcccgt
     1381 gagcatgacc catccaagcc tcccccagtc ctcctcatag cgatcgacca cgacggcgac
     1441 ctgcgcgttc tcagcgatat ttcgcaagcg tttgaggaga cggggcggct gacgcttcgg
     1501 ctttctatcg atcgtcacgt aaagcgtcct gcccgacacc acgaaacaga ccggcacgac
     1561 gtggggcacg ccggcgcgat cggcggtggc aagatgggca atcctgcgct cgcgcaagaa
     1621 ccgaagctcc aattctgcaa gcatcctggc ctcaataagg gacggtctct accggcgcca
     1681 gtctaacgca taggacgcct agctagccga gcgacgcgcc gcgcgagcgc agctcggggg
     1741 gtgcttcgcc accttgcccc gtcagccaca cgagatcggc gccaaaggaa tagatgagga
     1801 gcgccagggc gccgagacag aggggtgggg cggccgacgc cggcagaacc ggcaccaggg
     1861 tcgcgatcag cacgccgccc tgaatggcgg cgatgacctt gcgccgccgc agcggctgca
     1921 gcggcgccgc caacgtcggc catagccaac ccgcgccgac aaaaaggtag cgcatcagcc
     1981 cgcaactgag gacccaggct ccggtccgcc ccgccgcata aacgaggagc gacagcgcca
     2041 acacaaatag gcaatcgact tcaaggtcga aatccgcgcc gaattggctg accataccgc
     2101 tgcggcgggc gatccagcca tcgaccccgt cgagcagaag tgcagtcgtt gccgcaccgg
     2161 ccaacagcca accgagttcc gcatccagcg ccagcggacc cggcagggcc tccccgacaa
     2221 tgccccacaa tagggcggtc aacccggcgc gccccaaggt taccgagttg gccgtcccga
     2281 aacgctggtg gggggcgtgg cgtgccagat ccaacagcac aaaggtggtg accacaccgt
     2341 agcacgagaa ggctcccacc catccccgcc accccagctc caggggaagg cttaaggccg
     2401 ccgtcgccgc gctaacagcg gcccccatcg ccaccaactg gatccctgcc atggtgatga
     2461 ggggcatgcc gtgccaatgc cggcgcccga gaccgtgtgc gcggcccatt cccccgtggc
     2521 gttcccttcg caaggggcgc tcccgtatgg caatgtccct gccaggcggc ccagttcctc
     2581 cccgctcggc ccaaagctga ccgcccgacg acggcccccg ccgccatcct caatccttga
     2641 ccttgctgca acgccggcgg ctagcccgca cgcattcggc aaaaaaccgg ccgcacgcct
     2701 agcctgtcag cgcgaagcta tgtgcgtccc gcgctgcgga agcccttggc caccgttcca
     2761 gcggcttcga aggtggctgc cgcctccttg gccgtcatcg ccaccgtcgt ctcgatatcg
     2821 gagagactgc cgcccgccgc ggcggcaagg atggcggatg ccgctgcctt ctcgttgggg
     2881 aattcgccaa tcaccagcca atcgtgccgg ccgaacgtca aataccagcc cagcagcttg
     2941 ccgccgacgc tcttgaacag agtgccaacg gcgtcctcgc gattttcagg tttggcgagc
     3001 atgcccttca cggcgtcggt cgtaaacttt ccgcgcgaga tataaatggg catgggtttc
     3061 ctcctaaaga aaacacctac tcgaggccgt taacattatt ccctccggcg ggattggtca
     3121 gatcaactct ttgcaaccga aaagcgtttt tcgccgacgg gtttagagca atcaattcgg
     3181 tcgctctgcg cagcatgccg tcgacaaccc gaagaggtcg ccgcaaaggc tagggatggg
     3241 cgccacgcgc gcggccttat gcaagctccg agcactctgg catcgtgccc atcaacgtgc
     3301 gcgggctgtc gctggcacgc cgcccaatgc ccggcggaaa cgctcaaatg ccctcgctac
     3361 ggcgcgcccc gcccatgacc tctccagccg cccgtgcggt ccgtccgtcc ccaggtcgag
     3421 cgagcaccgt cgttgcacat cggggttaga cctgatttcc tttgtgccgg cgttgctgaa
     3481 atttctgggg ttgtgaggcc ctgaggccgg cggagaaccc ttgtgctgga cgcgagccga
     3541 aggggactcg gcgtggaggt aggcaggcaa gagaaaaacg gagctgccct gatgacgtcc
     3601 tcgcgcgcct cgaccccgcc ttcgcgacgc aaacaggaac tcattgtttt tgtgctcttg
     3661 agcctggtgt tggcgcccgt gctggccgtt gccacggtcg gcggctacgg cctggggatt
     3721 tgggtctatc aaatgtggcg cggaccaccg ggtccgccgc ccaaagcgcc gcgctcgccg
     3781 ggcgtcgttc cgcaatgacc tagccaatga ggctccacat gcgcctaacc cgccgcgcct
     3841 tgatctctgg ccaggctgag ccaggcccgg cgcctgctgg cgtcgaaatt gtcagtctcc
     3901 tcgtcagtgc gcggccgcaa ttcatgccgg ccgtcgccga agcgattttg gccttgggca
     3961 attgcgaaat tcacgcccgc gaccccaggg gcaagctgat cgtggtgctc gaagaggaga
     4021 gcacgggggc cgtcggcgcc aaaatcaata cgatttccgg cctgccgcac gtgctttcgg
     4081 cggtcatggt gttccaggcc accgatgctg atgcgaccat aggctgaggc acgccatgac
     4141 cgatgcagca ctgaaccgcc gccaactcct caaaacccaa gctgcgatgg cggccgcggc
     4201 ggtggcggga ttgccgacat cgtcggtggc ccaattgatc accgaccgca aggaaagcga
     4261 gctcgcctgg aacaaggcgc cgtgccgatt ttgcggcacg ggctgcggcg tcctcgttgc
     4321 caccaaagat ggtcgcgtgg tggcgaccca tggcgatgcc aaagcggaag tcaatcgcgg
     4381 gctcaattgc gtcaagggct attttctttc caaaatcatg tacggagagg atcggttgcg
     4441 cgagccgttg ctgcgcaaaa agaacggcaa gtatgacaag agcggagagt tcactgccgt
     4501 ttcctgggac gaagccttca cggtcatggc cgagcagttt cgcgccgcct tgaagaaagg
     4561 cgggccgtcg gcggttggca tgttcggctc cggccaatgg acgatctggg aaggctatgc
     4621 ggcggccaag ctgttcaagg ccggcttccg cagcaacaat ctcgatccca acgcgcgcca
     4681 ttgcatggcc tccgccgccg ccggcttcat gcgttccttc ggcatcgacg aacccatggg
     4741 ctgctacgac
//
LOCUS       DASTMT010000071         1598 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_1353111, whole genome shotgun sequence.
ACCESSION   DASTMT010000071 DASTMT010000000
VERSION     DASTMT010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 1598)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1598)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1598
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1353111"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..329)
                     /locus_tag="VFR00_01520"
     CDS             complement(<1..329)
                     /locus_tag="VFR00_01520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0157955.1"
                     /translation="MDEKKLRRAIEQLLESQPDNVRLRDHLEGLRRDPLFPGLTWFWG
                     PRLYARSRATFRPFIVNHFSEWLAVGKRWQRVSWADHAADLEAWLQAVRAARDGALVR
                     RLQRWKYA"
     gene            complement(525..>1598)
                     /locus_tag="VFR00_01525"
     CDS             complement(525..>1598)
                     /locus_tag="VFR00_01525"
                     /inference="COORDINATES: protein motif:HMM:NF020976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HEU0157956.1"
                     /translation="EQPDLVIFLGDYIYEYTIAATSASAGRILRPHDGPTATDLAGYR
                     NRYALYRTDPDLKALHAAAPCLVTWDDHEVENDYADQWSQDMRVEPAIFLARRAAAYQ
                     AYREHMPLRPRPSGEPHAFRLYGRIPYGTLVSFSMLDGRQYRSKQPCEVPPWRRGHVA
                     PLSCTERLEPQRTLLGEAQEAWLLQSFKRTETAWNVLAQGQLMAELRQRSRVGEPGFW
                     TDGWDGYPTARQRLLEAMVASRLANPVVIGGDIHSFWATDLKADFNAPGSPTVATEFV
                     GTSITSDPPPYGLIAELLPENPHVRYFESRHRGYVAVDLDRERMRVRMRSISDRRDPR
                     ATVATLKQFVVMTGAAGAIESNG"
BASE COUNT          276 a          520 c          555 g          247 t
ORIGIN      
        1 gcgtacttcc agcgctgcag gcggcggaca agggcgccat cgcgcgccgc gcgcaccgcc
       61 tgcagccagg cctcgaggtc cgccgcatgg tcggcccagg acacgcgctg ccagcgcttg
      121 ccgacggcga gccactcgga aaaatgattg acaatgaacg ggcgaaaggt cgcccggctg
      181 cgcgcataga gccgcggtcc ccaaaaccag gtgagaccgg gaaacaacgg atcgcgacgc
      241 aggccttcca ggtggtcgcg cagacggaca ttgtccggtt ggctctcgag cagttgctcg
      301 atcgccctgc gcaacttctt ttcgtccatc gctgagctag cacccctaat acgcgacaaa
      361 ggcgcacccg gcccaacgct agcagaggaa atctgacaga atttgtcggc gccgggccgc
      421 gccggtgcgc cggggccggc cgtgcccaaa tcgccacacc ggcgggctca acgggacgat
      481 ggccacggtc atggcgcggc acgccggcgc tggcccgcgc tcggctagcc gttgctctcg
      541 atcgcgccgg cggccccggt catgaccacg aattgcttga gcgttgccac cgtcgcccgg
      601 ggatcgcgcc gatccgagat gctgcgcatg cgcacgcgca tgcgctcgcg atcgaggtcg
      661 acggcgacgt agccgcggtg gcgactttcg aaatagcgca cgtgcgggtt ttcgggcaag
      721 agctcggcga tcagaccata gggcggggga tctgaggtga tggaggtgcc gacgaactcc
      781 gttgccaccg tcggcgagcc tggcgcgttg aagtctgcct tgaggtcggt cgcccagaag
      841 gaatggatgt cgccgccgat cactaccgga ttggccaggc ggctggcgac catcgcctcg
      901 agcaggcgtt ggcgggccgt gggatagcca tcccagccgt ccgtccagaa cccaggctcg
      961 ccgacccggc tgcgctggcg caactcagcc atcaactggc cctgggccag cacgttccag
     1021 gccgtctcgg tgcgcttgaa gctttgcagc agccatgcct cctgcgcctc gcccagcaag
     1081 gtgcgctgag gctcgagacg ttccgtgcag ctcaacggcg ccacatggcc ccgtcgccat
     1141 ggcggtacct cgcagggttg cttggagcga tattgacggc catccagcat cgagaaggac
     1201 accagcgtgc cataggggat gcggccgtag agacggaagg catgcggctc gccgctcggc
     1261 cgcggccgca gcggcatgtg ttcgcgatag gcctgatagg cggcggcgcg ccgcgccaag
     1321 aaaattgcgg gctccacgcg catgtcctgc gaccattgat cggcatagtc gttttccacc
     1381 tcatggtcgt cccaggtcac caggcacggg gcggcggcgt gcagcgcctt gagatcggga
     1441 tcggtgcggt agagggcgta gcggttgcga tagccggcaa ggtcggtggc ggtggggcca
     1501 tcgtgcgggc gcaaaatgcg ccccgccgag gcggacgtgg cggcaatcgt atactcgtag
     1561 atgtagtcgc ccaagaaaat gacgaggtcg ggctgctc
//
LOCUS       DASTMT010000072         4574 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_3322283, whole genome shotgun sequence.
ACCESSION   DASTMT010000072 DASTMT010000000
VERSION     DASTMT010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4574
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3322283"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1611
                     /locus_tag="VFR00_01530"
     CDS             <1..1611
                     /locus_tag="VFR00_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018261278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEU0157957.1"
                     /translation="GGGFGSKIFIYAEETVCLWASRKIGRPVKWTGERSEAFLADAHG
                     RDHATRAELAIDASGKILALRASTKANLGGYLSTFASSVPTYLYAPLLSGQYDIPAIY
                     CEVDGVYTNTAPVDAYRGAGRPEATFVVERLVEVAARQIGKDPAEFRRQNFIRSFPHQ
                     TPVIMAYDAGDYEACLNKALELADYKGLGARKAESARKGLKRGIGFSAYIEACGIAPS
                     QAVGSLGAGVGLWESAEVRVNPTGSVEVLTGSHSHGQGHETTFAQLVSQRLGIPIEQV
                     TVVHGDTDKVQFGMGTYGSRSGAVGMSAIFRAIDKVIVKAKKVAGFVLEVPEDSIDFN
                     DGIFSSKATNKTLTFPEVALQAYIAHKFTGAQLEPGLKEGAFFDPTNFTFPSGVHVCE
                     LEIDPATGATRIDRWTAVDDFGTLVNPMIVEGQVHGGIVQGVGQALLEGAVYNKEGQL
                     VTASFMDYTMPRASDVPSFKVAMTNTPCPSNPLGVKGCGEAGAIAAPAAVVNALTDAL
                     GHEEIAMPATPQAVWRAASKSAEKLAAE"
     gene            1634..2431
                     /locus_tag="VFR00_01535"
     CDS             1634..2431
                     /locus_tag="VFR00_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006021303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HEU0157958.1"
                     /translation="MYAFDYHRPSSVAEAQRLLASSQEGKLLAGGHTLLPTMKLRLAG
                     PKMIIDLCRISELRGIERKGNDLVIGAMETHDAVASSPVVGSSLAGLAQLAAHIGDQH
                     VRHRGTIGGSVANNDPAADYPAAVLALNAKVITNKREINADDFFKGLFSTALGPDEII
                     TRIAFPIPSKFGYAKFANPASRYALVGVAVAQTPGGIRVAVTGASSSGVYRAANMEAA
                     LGKGWSAQSLGTLAGEAANMLSDIHGDAKYRAHLVGVMARRAVAAAT"
     gene            complement(2466..3662)
                     /locus_tag="VFR00_01540"
     CDS             complement(2466..3662)
                     /locus_tag="VFR00_01540"
                     /inference="COORDINATES: protein motif:HMM:NF019640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEU0157959.1"
                     /translation="MNDKNPAPHSPSLAADPVARVASIADVLRRAAPENEKLGRLTPA
                     VVDKLHEQRLFRLLLPAAYGGDEADLATWFRTMEAIAKNDASTAWCVGQINGCAASSA
                     ALAPEIAQKMWGENARAALSWGPPIQSRAEETEGGHLLTGEWGMSSGSRHASWLGLMA
                     PVFDRAGKPVPLPQGVSARIFFVPTDAVEWIDNWDVIGLNATCSGGYRLAGRFVPAGY
                     SFSREHLPDVRLPGALFKFPLNSLFATGFSGVSLGIARAMLNEVMTLAREKTPRLAKS
                     TLRDNHHVQFQIGDAEARLRSARHYVESTVTRVWEEVVSSGKLTIERRIDIRMAATFA
                     IHEANKVADTVWQIAGATAIFSSSAFERRLRDIKTLTQQVQGRQSHLQDVGAYLMGLE
                     PSLAFA"
     gene            complement(3652..>4574)
                     /locus_tag="VFR00_01545"
     CDS             complement(3652..>4574)
                     /locus_tag="VFR00_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007756292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU0157960.1"
                     /translation="AQALDKAMPHLTTDDHVRLYYEEVGSGIPVVFVHEFAGDYRSWE
                     PQVRYFARRYRAIAFNARGYPPSDVPAPGPAYSQARAAADIRCVLDALKIDKAHVVGL
                     SMGGLAVLHFGLTYPERARSLLVGGAGYGSERGEREKFRTEASTIADRLEREGMARFA
                     EAYAYGPTRVQFEGKDPRGFAEFKAMLAEHSATGSIGTQLGVQRERPSLFDLEDKLKA
                     LKVPMLVVTGDEDWPCLVPNLYLKRVCTSAALLVLPNSGHAVNLEEPGLFNAALADFL
                     AQVDAGRWPVRDPRAISQSITGMTQASNER"
BASE COUNT          817 a         1539 c         1467 g          751 t
ORIGIN      
        1 ggcggcggtt tcggctccaa gatcttcatc tacgcggaag agaccgtgtg cctgtgggcc
       61 tcgcgcaaga tcggccggcc ggtcaaatgg accggcgagc gcagcgaggc cttcctcgcc
      121 gatgcgcacg gccgcgacca cgccacgcgc gcggagctgg caatcgatgc cagcggtaag
      181 atcctggcgc tgagggcgag caccaaggcc aatctcggcg gctatctttc gaccttcgcc
      241 tcctcggtgc cgacctatct ctacgcaccc ctgctgtcgg ggcagtacga tatcccggcc
      301 atctattgcg aggtcgatgg cgtctacacc aacaccgcac ccgtcgatgc ctatcgtggg
      361 gccgggcggc ccgaggcgac cttcgtggtg gagcgcctgg tcgaggtggc cgcgcgccag
      421 atcggcaagg atccggccga attccgccgc cagaacttca tccggtcctt cccgcatcag
      481 accccggtga tcatggcgta cgacgcggga gactacgaag cgtgcctcaa caaggcgctc
      541 gagctcgccg actacaaggg tctcggcgcc cgcaaggccg agagcgccag gaaaggcttg
      601 aagcgcggca ttggcttctc cgcctatatc gaggcctgcg gcatcgcgcc cagccaagcc
      661 gtgggctcgc tgggcgccgg cgtcggcctt tgggagtctg cggaagtgcg cgtcaatccc
      721 accggctcgg tcgaggtgct caccggctcg catagccacg gccaggggca tgagacgacg
      781 tttgcccagc tcgtgtcgca gcgcctcggc atccccatcg agcaggtcac ggtcgtgcac
      841 ggcgataccg acaaggtgca gttcggtatg ggcacctacg gctcacgctc cggcgcggtc
      901 ggcatgtcgg ccatcttccg tgccatcgac aaggtgatcg tcaaggccaa gaaggtggcg
      961 ggtttcgtgc tggaggttcc cgaagacagc atcgacttca acgatggcat cttctccagc
     1021 aaggcgacca acaagacgct cacctttccc gaggtggcgc tgcaggccta catcgcccac
     1081 aaattcacgg gcgcgcagct cgagcccggc ctgaaggaag gcgccttctt cgatcccacc
     1141 aatttcacct tcccctcggg cgtgcacgtc tgcgagctcg agatcgaccc ggcaacgggg
     1201 gcaacccgca tcgatcgctg gaccgccgtg gatgatttcg gcaccctcgt caatcccatg
     1261 atcgtcgagg gccaggtgca cggcggtatt gtccagggcg tgggacaagc cttgctcgag
     1321 ggcgccgtct acaacaagga aggtcagctc gtcaccgcca gcttcatgga ttacaccatg
     1381 ccgcgggcca gtgacgtgcc ctccttcaag gtggcgatga ccaatacgcc ctgtccctcc
     1441 aatccccttg gcgtcaaagg ttgcggcgag gccggtgcca ttgccgcccc ggccgccgtc
     1501 gtcaacgcgc tcacagacgc gttgggacac gaggagatcg ccatgccggc gacaccgcag
     1561 gccgtgtggc gggccgccag caaatcagcc gaaaagcttg ccgccgaata gggcgaaaac
     1621 ggaggaccgg atcatgtatg ccttcgacta tcatcgcccg agctcggttg ccgaggcgca
     1681 aaggctgctg gcgagttcgc aagagggtaa gctcctagcc ggcgggcaca cgctgctgcc
     1741 gaccatgaag ctgcggcttg ccggaccgaa gatgatcatc gacctttgcc gcatctccga
     1801 gctgcgcggc atcgagcgca agggcaacga tctcgtcatc ggcgccatgg agacgcacga
     1861 cgcggtggcg agctcgccgg tcgtcggcag cagccttgcc ggcctcgcgc agctggccgc
     1921 gcatattggt gaccagcacg tgcgccaccg cggcaccatc ggcggatcgg tcgccaacaa
     1981 cgacccggcc gccgactatc ccgccgcggt gctggcgctc aacgccaagg tcatcaccaa
     2041 caagcgcgag attaacgccg acgacttctt caaggggctg ttctcgaccg cgctcggccc
     2101 agacgagatc atcacgcgca ttgccttccc gatccccagc aagttcggct acgccaaatt
     2161 cgccaatcca gcctcccgct atgctttggt gggcgtcgcc gtcgcgcaga cccccggcgg
     2221 catacgcgtg gcggtcacgg gagcgtcctc ctccggcgtc tatcgcgccg ccaatatgga
     2281 ggccgcgctg ggcaaaggct ggtcggcaca gtcgctgggc acgctcgcgg gcgaggccgc
     2341 gaacatgctg agcgatatcc acggcgatgc caagtaccgc gcccacctcg tgggcgtcat
     2401 ggcccgacgg gcagtcgcgg ccgctaccta actggcaatg gcccgcgccg gcgggccatg
     2461 ccggctcagg caaacgccag gctgggctcg aggcccatca ggtaggcgcc gacatcctgc
     2521 aggtgggatt ggcgcccctg cacctgctgg gtgagggtct tgatgtcgcg caggcgtcgc
     2581 tcgaaggcgc tcgaggaaaa aatcgccgtg gcaccggcga tctgccaaac cgtatcggcc
     2641 accttgttcg cctcgtgaat ggcgaaggtg gctgccatgc ggatgtcgat ccgccgctcg
     2701 attgtgagct tgccggagct caccacctcc tcccacaccc gcgtgaccgt actctccacg
     2761 taatggcgcg ccgagcgcag gcgcgcctct gcgtcaccga tctggaattg cacatgatgg
     2821 ttgtcgcgca aggtcgattt ggcgagccgc ggggtcttct cccgtgccag cgtcatcact
     2881 tcattcagca tggcgcgggc aatccccaag ctgacgccag agaacccggt agcgaagaga
     2941 ctgttgaggg gaaacttgaa gagcgcgccc ggcaagcgca catcgggcaa atgttcgcgg
     3001 ctgaaggagt agccggcggg cacgaagcgc cctgcgagcc gatagccacc gctgcaggtg
     3061 gcgttgagcc caatcacgtc ccaattgtcg atccactcga ccgcatcagt cggtacaaag
     3121 aagatgcgcg cactcacgcc ctgcggcagc ggcacgggct tgccggcacg atcgaacacc
     3181 ggcgccatca aaccgagcca gctggcgtgc cggctcccgc tcgacatccc ccactcgccc
     3241 gtcaggagat gaccaccctc ggtttcctcg gcacgcgact ggatcggcgg tccccagctc
     3301 agcgcggcac gtgcgttctc gccccacatt ttctgcgcga tctctggcgc gagtgccgcg
     3361 gaggaggcgg cgcagccgtt gatctggccg acacaccagg ccgtgctggc gtcatttttg
     3421 gcaatggcct ccatggtccg gaaccaggtg gcgagatcgg cttcgtcccc accgtaggca
     3481 gcaggcagca gcaaccggaa cagacgctgc tcgtgcaatt tgtcgaccac cgcgggcgtc
     3541 aaccggccga gcttttcgtt ttccggtgcc gccctgcgca agacgtcggc gatgctggcc
     3601 acgcgcgcga ccgggtcggc ggcgagcgag ggcgagtggg gggcaggatt tttatcgttc
     3661 attggaggcc tgcgtcattc cggtaatact ctggctgatg gcacgcggat cgcgcacggg
     3721 ccagcgcccg gcatcgacct gggccaaaaa gtcggccagc gcggcgttga aaaggccggg
     3781 ttcctcgagg ttcacggcat gcccgctatt gggcaggacc agcagcgccg ccgacgtgca
     3841 aacgcgcttc agatagagat tggggaccag acagggccag tcctcgtcgc cagtcaccac
     3901 cagcatcggc actttcaggg ctttgagctt gtcttccaga tcgaacagcg agggccgctc
     3961 gcgctggacg ccgagttgcg ttccgatgct gccggtggcc gaatgctcgg ccaacatcgc
     4021 cttgaattcg gcgaacccgc gtgggtcctt gccctcgaac tgtacgcgcg tcgggccata
     4081 ggcgtaggcc tcggcgaacc tcgccatacc ctcgcgttcc agccggtcgg cgatggtgga
     4141 cgcctcggtt cgaaactttt cccgctcgcc gcgctccgaa ccgtagcccg ccccgcccac
     4201 caacagcgag cgcgcccgct ccggataggt gaggccgaaa tgcaagacgg caaggccgcc
     4261 catggagagg ccaacgacat gggccttgtc gattttcagt gcatccagca cgcagcggat
     4321 atcggcggcg gcgcgcgcct gtgaataggc cggtcctggc gctgggacat cggagggcgg
     4381 gtagccacgg gcgttgaagg caatcgcgcg atagcgacgc gcgaaatagc gcacctgcgg
     4441 ctcccagctg cgataatcgc cagcgaactc gtgcacgaaa acgacgggga tgccgctgcc
     4501 gacctcctcg tagtagagcc gcacatgatc gtcagtcgta agatggggca tggccttgtc
     4561 caacgcttga gcct
//
LOCUS       DASTMT010000073         4289 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_2010309, whole genome shotgun sequence.
ACCESSION   DASTMT010000073 DASTMT010000000
VERSION     DASTMT010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4289
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2010309"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..444
                     /locus_tag="VFR00_01550"
     CDS             <1..444
                     /locus_tag="VFR00_01550"
                     /inference="COORDINATES: protein motif:HMM:NF019552.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HEU0157961.1"
                     /translation="AKGVLRFAWDLPFAVHFHVHPNVEVWPGREAHTADLVTQSGSHW
                     RLSATAAALSIEESVHNARSLGPHPAQQVVLRATCHGEAKLSWQLERLPPGREPGQRL
                     ADSTALAPSRGERLVQTEQDAQASPAEAGEDRRADGPAAPPDPQG"
     gene            545..2131
                     /gene="purH"
                     /locus_tag="VFR00_01555"
     CDS             545..2131
                     /gene="purH"
                     /locus_tag="VFR00_01555"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEU0157962.1"
                     /translation="MPEANKICRALLSVSDKQGLLEFARGLLAFGVEILSTGGTANAL
                     KAAGIGVKDIAEHTGFPEMMDGRLKTLHPKVHGGLLAVRGNAEHQAAAVAHGIAPIDL
                     LVVNLYPFEATVAAGAAFDVAIENIDIGGPAMLRAAAKNHAAVTVVVDPEDYALVLDE
                     MGAQAGGVSAALRQRLAAKAFARTAAYDAVISGWFADVLGEAAPAWRAFGGKLTQALR
                     YGENPHQRAAFYASAAGRPGLASLVQHQGKELSYNNLADTDAALELVAEFDRASPAVA
                     IIKHANPCGVATGATLLEAYRQALRCDPVSAFGGIIALNGVIDAAAAAEITSIFTEVV
                     IAPEASPEAKAIFAGKKNLRLLTTGALPDPRRSEVTFRSLAGGFLVQSRDGAVIADLD
                     LRVVSKRQPTAQELADLKFAFTVAKHVKSNAIVYAKGGATVGIGAGQMSRVDSARIAA
                     WKSEEAARAAGLPEPLAKGSVVASDAFFPFPDGLLAAAEAQATAVIQPGGSLRDKDVI
                     AAADAHGLAMVFTGVRHFRH"
     gene            2137..2643
                     /locus_tag="VFR00_01560"
     CDS             2137..2643
                     /locus_tag="VFR00_01560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0157963.1"
                     /translation="MAERSQPLAVRLRPATAEDGFLLRRWLNDPAIAACGGNRASWEA
                     EIALARLSETALRRIVTVAARPVGYAQALELGLRAGTQPPEIPTGTWDIEVWSMPSRP
                     RVLAAVPALLAEEVFATTLAVACSALVSIRQETMVRAYEQAGFRWRRIWHDPLFGPAW
                     LLLKERPH"
     gene            complement(2528..>4289)
                     /locus_tag="VFR00_01565"
     CDS             complement(2528..>4289)
                     /locus_tag="VFR00_01565"
                     /inference="COORDINATES: protein motif:HMM:NF044969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004352 - glutamate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0019551 - glutamate catabolic process to
                     2-oxoglutarate [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-glutamate dehydrogenase domain-containing
                     protein"
                     /protein_id="HEU0157964.1"
                     /translation="AKSIALSPEIAALIGLSAAKLTPNEVMHALLKSKTDLLWFGGIG
                     TYVRASGESDADVGDRANDAIRVSAAELGAKVVGEGANLGMTQRARIEFAQRGGRLNT
                     DFIDNSAGVNTSDQEVNIKIALGPASRSGRLAASQRKLLLAGMTEAVASRSLANNYQQ
                     SLALSLAERSSHGELPDYALLLHSLEERGLFERGLEGLPSDKELEERGRAGRGLTRPE
                     LAVLLSYAKIALEHDLIGSTVPDEPLLSRWLDAYFPDELRRRFAEGIAGHSLRREIIA
                     TALTNAVLNRGGPALAVRLADETRRTTPDVALAFLAVREVFELPALWSGLDALDGKLS
                     GATQIALYQRTRDLINTATLFMLRHGGSDGTLADLIARQSQTLATLKSVLPTVLPARL
                     AKGMADEASRLIALSVPQDLAREIAGLDVSEHSLAIDAVAQATGSPVAEAARVFLVLG
                     DRLRISKLASKAGRIVTPDYYDRLAVAQALNQLGKSQADLAQAAIRAGGATAIEAWLS
                     QQGPRLARVIGNLEAIANEGTLTLSRLLVAAGQLQDVAGAASAAAPSAATRRARTGGR
                     ARSGASETQPARKRAPSSPA"
BASE COUNT          642 a         1446 c         1515 g          686 t
ORIGIN      
        1 gccaagggcg tcctgcgatt tgcctgggat cttcccttcg ccgtgcattt ccatgtccat
       61 cccaacgtcg aggtctggcc cgggcgggag gcgcacacgg ccgacctcgt cacccagtcc
      121 gggtcgcatt ggcgcctctc ggccacggct gccgcgctga gcattgaaga gagcgtgcac
      181 aatgctaggt cgctcgggcc gcatccggcg cagcaagtgg tgctcagggc gacctgccac
      241 ggcgaggcca agctcagctg gcagctcgag cgcctgccac cgggtcggga acccggccag
      301 aggctggccg acagcaccgc gctggcgccc tcgcggggcg aacggctggt ccagacggaa
      361 caggacgcgc aggcctcgcc ggccgaggcc ggcgaggatc gccgcgcgga tggcccggca
      421 gcaccgccag atccacaggg ttgagcggcg cctgttgcca agctcggttc catgctaagc
      481 gcgcggcgga ggcctgcggg ccggcgcgcg agcgcggccc gagcaaattg ggagtggacg
      541 tccgatgcca gaggccaaca agatctgccg cgcgttgctg tccgtttccg acaagcaggg
      601 actgctggag tttgcgcgcg ggctcttggc gttcggggtc gaaatcctct cgaccggggg
      661 cacggcgaac gcgctcaagg ctgcggggat tggcgtcaag gacatcgccg agcacacggg
      721 cttcccggaa atgatggatg gccgcctgaa gacactccat cccaaggtgc atggcgggct
      781 cttggccgta cgcggcaacg ccgagcacca ggccgcggcc gttgcacacg gtattgctcc
      841 catcgatctg ttggtggtca acctttaccc gttcgaggcc acggtggccg cgggcgccgc
      901 cttcgatgtg gctatcgaaa acatcgatat cggcggtccg gccatgctgc gcgccgccgc
      961 caagaaccac gcagcggtca ccgtcgtcgt cgatcccgag gattacgccc tcgtgctgga
     1021 cgagatgggc gcgcaggcgg gcggcgtcag cgccgcgctg cgccaaaggc tggctgccaa
     1081 ggcgtttgcg cgcaccgccg cttacgacgc cgtcatcagc ggttggtttg ccgacgtcct
     1141 gggcgaggct gcgccggcct ggcgcgcctt tggtggcaag ctgacccagg ccctgcgcta
     1201 tggcgagaac ccgcaccagc gcgccgcctt ctatgcctcc gccgcggggc gcccggggct
     1261 cgcctcgctc gtgcagcatc aaggcaagga gctgtcctac aacaatctgg ccgataccga
     1321 cgcagccctc gagttggtgg ccgagttcga tcgggccagc cccgcggtcg ccatcatcaa
     1381 gcacgccaac ccctgcgggg tggcaaccgg ggcgacgctg ctggaggcct atcgccaggc
     1441 gctgcgctgc gatcccgtca gcgccttcgg cggcatcatc gcgctcaacg gcgtcatcga
     1501 cgcggccgcg gcggcggaga tcacctcgat cttcaccgag gtggtgatcg cgcccgaggc
     1561 cagcccggaa gcgaaggcga tttttgccgg caaaaagaac ctgcgcttgc tcaccaccgg
     1621 ggcgctgccc gatccgcgcc gcagcgaggt taccttccgc tcgctggcag gcgggttttt
     1681 ggtgcagtcg cgcgatggcg ccgtaattgc tgatctcgac ctgcgggtag tcagcaagcg
     1741 ccagcccacg gcgcaggagc tcgccgacct caagtttgcc ttcacggtcg ccaagcacgt
     1801 caaatccaat gccatcgttt atgccaaggg cggcgccacc gtgggtatcg gcgccgggca
     1861 gatgagccgg gtcgacagcg cccgcatcgc cgcctggaaa tccgaagagg cggccagggc
     1921 ggcgggtctg cccgagcctt tggcgaaggg ttcggtggtg gcctccgacg cctttttccc
     1981 cttccctgac ggcctgttgg cggccgccga ggcccaggca acggccgtca tccagcccgg
     2041 cggttcgctg cgcgacaagg acgtgatcgc cgcggcggac gcccacggcc tggcgatggt
     2101 cttcaccggc gtgcgccatt tccggcattg aacggcatgg ccgagcggtc ccagcccctg
     2161 gcagtccggc tgcgcccggc cacggccgaa gacggcttct tgctgcgccg ctggctcaac
     2221 gatcccgcca ttgccgcgtg tgggggcaac cgggcaagct gggaggcgga gattgcgctg
     2281 gcgcggctca gcgaaacggc gctgaggcgc atcgtcaccg ttgccgcgcg tcccgtcggc
     2341 tacgcacagg ccctcgaact ggggctgagg gccggcacgc aacccccgga aatccccacg
     2401 ggcacttggg acattgaggt ctggagcatg ccctcgcgcc cacgcgtgct ggccgccgtc
     2461 ccggcgctgt tggcagagga ggtgttcgcc accacgctcg cggtcgcctg ctcggcgctg
     2521 gtttcgatca ggcaggagac gatggtgcgc gcttacgagc aggctgggtt tcgctggcgc
     2581 cggatctggc acgaccccct gttcgggccc gcctggttgc tgctgaagga gcggccgcac
     2641 tagcggcgcc ggccacgtcc tgcagttgtc cggcggcgac cagcaggcgg gacaaggtga
     2701 gcgttccctc gttggcgatc gcctcgaggt tgccaatcac ccgcgccaag cgcgggccct
     2761 gctgggagag ccaagcctcg atcgccgtcg cgccgccggc gcggatggcc gcctgcgcca
     2821 ggtcggcctg cgattttccc agttgattga gcgcctgggc gacggcgagc cggtcatagt
     2881 agtcaggtgt cactatgcgg cctgccttgc tggcgagctt gctgatgcgc aggcggtcgc
     2941 ccaacacaag gaagacgcgc gcggcttccg ccacggggct ccccgtcgct tgcgcgaccg
     3001 cgtcgatggc cagcgaatgc tccgatacat cgaggccggc gatctcgcgg gcgagatcct
     3061 gcggcacgct gagcgcgatc agccggcttg cctcgtcggc catgcccttg gccaggcgcg
     3121 ccggcagcac ggtcggcaaa accgacttga gggtcgccag cgtctgcgat tgccgggcga
     3181 tgagatcggc gagcgtgccg tcgctgccgc cgtggcgcag cataaagagc gtggccgtgt
     3241 tgatgaggtc ccgggttcgc tgatagaggg cgatttgcgt ggctcccgac agtttgccat
     3301 cgagggcatc gagcccgctc cagagcgccg gtagctcgaa cacttcgcgc acggccagga
     3361 aggccaaggc cacatcgggc gtcgtgcggc gcgtctcgtc ggcgagccgc accgccagcg
     3421 ccgggccacc gcgattgagg acggcgttgg tgagcgcggt ggcgatgatt tcgcggcgca
     3481 ggctgtggcc ggcgatgcct tcggcaaagc gccggcgcaa ctcatcggga aaataggcat
     3541 cgagccaacg ggagagcagc ggctcgtccg gcacggtgct gccaatcagg tcgtgctcga
     3601 gggcaatctt ggcatagctc aacagcaccg ccagctccgg ccgggtgagg ccacgcccgg
     3661 cgcggccacg ctcttccagc tccttgtcgg agggcaagcc ttcgagcccc cgctcgaaca
     3721 agccgcgctc ctcgaggcta tgcagcagga gggcatagtc ggggagctcg ccgtgcgaac
     3781 tcctctccgc caggctcaac gccagggact gttggtagtt gttggcgagc gagcggctgg
     3841 ccacggcctc ggtcatgccg gccaacagga gcttgcgttg gctggcggcg agcctgcccg
     3901 aacggctggc cggccccagc gcgatcttga tgttcacttc ctggtcggag gtgttcaccc
     3961 ccgccgaatt gtcgatgaaa tcggtgttga ggcggccgcc gcgctgggcg aactcgatgc
     4021 gggcgcgttg ggtcatgccg aggttggcgc cttcgcccac gaccttggcg ccgagctcgg
     4081 ccgcgctgac gcggatggca tcattggcgc ggtcgccaac gtcggcatcg ctctcgccgc
     4141 tggcgcgcac gtaggtgccg atgccgccga accagaggag gtcggttttg ctcttgagca
     4201 gggcgtgcat tacctcgttg ggagtgagct tggccgcgct gaggccgatg agagcggcga
     4261 tctcggggct gagtgcaatc gacttggcc
//
LOCUS       DASTMT010000074         2207 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_3979686, whole genome shotgun sequence.
ACCESSION   DASTMT010000074 DASTMT010000000
VERSION     DASTMT010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2207
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3979686"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            36..533
                     /locus_tag="VFR00_01570"
     CDS             36..533
                     /locus_tag="VFR00_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013420614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HEU0157965.1"
                     /translation="MAFEIERKFLVRGQEWRRLASVAMRLRQAYLAAGDKSSTRVRIK
                     DDRSASVTIKAKGTGLRRLEVEYPISLIDGEALMSLRQSGLIEKVRHRVPWHHHTWEV
                     DVFGGDNAGLILAEIELRDEREAFERPPWLGLEVTGQPRYYNSSLAQRPYAGWQAAAP
                     AVVAG"
     gene            706..1062
                     /locus_tag="VFR00_01575"
     CDS             706..1062
                     /locus_tag="VFR00_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011441676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
                     /protein_id="HEU0157966.1"
                     /translation="MLIRAASVCALQLALLHVVSLASAEAQQAPAGVPGVVTGGSAST
                     SIGGQAAARKGDTTDAGGAIIEGSKDVFIDGKPAAVLGDKTNCGGITIGGAGNVFING
                     KPAARTGDLTTGCAGK"
     gene            1092..>2207
                     /locus_tag="VFR00_01580"
     CDS             1092..>2207
                     /locus_tag="VFR00_01580"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU0157967.1"
                     /translation="MRLLSWRWWRFVAVSLLSWALLVPAEAQQASKELSDLRETAGDL
                     YKSGDFARSLRFYERATPLVMREFGAEHEQMAIHYHSLGLVAEAAGDLAAAERYYRAA
                     IPIREKVYGPDSAGTAMALDQLAAVYLKMGRADAADPLVKRASQIRQDIGALLGPNHA
                     FFASDHANRGDLGLARGDWPAALDSYREAIRLLTGQDTSQTVVQSVVQEEIKRYRETF
                     IGLCRAAWQMRSTPGANASSLFEETFAAAQLAWATSAASALAKMSTRIGAGSTPLGQR
                     IRRVQDLSDRVLRLNGEDNALLSNWSAVQRQDARYSAVQEEFRAASLARGKATAPTVK
                     RQTQLVQELTGLVQRCPPGQNKAGCEGSEAEIKAISQQ"
BASE COUNT          391 a          729 c          740 g          347 t
ORIGIN      
        1 ctagaatggc gccattgtga cagcgggcgg ggatcatggc ttttgaaatc gagcggaagt
       61 tcctggtgcg cggccaggaa tggcgcagac ttgcgagcgt tgcaatgcgc ctgcggcaag
      121 cctatttggc cgccggcgac aagtcctcga cgcgcgtgcg catcaaggat gacaggtcgg
      181 ccagcgtgac cataaaggcc aagggcacgg ggctgcgccg gctcgaggtc gagtatccga
      241 tctccttgat cgatggcgaa gcactgatgt cgttgcgcca atcgggcctg atcgaaaagg
      301 tccgccaccg agtgccctgg catcaccaca cctgggaagt cgatgtcttc ggcggcgaca
      361 acgccggtct gatcctggcc gaaatcgagc tgcgcgacga gcgcgaggcc tttgagcggc
      421 ccccctggct cggcctcgag gtgacaggtc agccgcgcta ctacaacagt tcactggcgc
      481 agcgccccta cgcgggctgg caggcggcgg cgccagcggt cgtcgccggc tgacggcccg
      541 gccgggcggc acggccggcc gacatcggcc ctttcggccc aaagctcgac gacaaggcgc
      601 cccttgcatg cagccccaat ccgcgcgacc gtgcttgccg ggcaggctcg gggacaccgg
      661 acggccgaag ctcacggtcg ctccgaccaa ggcgcggagg gaagcatgtt gatacgagcc
      721 gcatcggttt gcgccctaca gttggcgctc ctgcacgtgg tgagcctcgc cagtgctgag
      781 gcgcagcagg ctcctgccgg cgtaccgggc gtggtcacgg gtggcagcgc ctcgacctcg
      841 atcggtggcc aggccgcggc gcgcaaaggc gacaccaccg atgccggcgg cgccatcatc
      901 gaaggctcaa aagacgtgtt catcgacggc aaaccggccg cggtgctggg cgacaaaacc
      961 aactgcggcg gcatcaccat tggtggggcc ggcaacgttt tcatcaacgg taagccggcg
     1021 gcccggaccg gcgacctcac gacgggatgt gcgggcaaat agccgaccgt ctggcacctc
     1081 gggggattgc tatgaggctc ctgtcctggc gctggtggcg cttcgttgcc gtgtctttgt
     1141 tgagctgggc gttgctggta ccggccgagg cacaacaggc ctccaaggag ctgagcgacc
     1201 tgcgcgagac ggccggcgac ctctacaaaa gtggcgactt cgcccggtcc ctgcgcttct
     1261 atgagcgcgc cacgccgctg gtaatgcgcg aatttggcgc cgagcacgaa cagatggcga
     1321 tccactacca ctcgctcggc ctggtggcgg aggcggccgg cgacctggcg gccgccgaga
     1381 ggtactaccg cgccgccatc cccatccgcg agaaggtgta cggccccgat agcgccggca
     1441 ccgcgatggc gctcgatcag ttggcggctg tctatctcaa gatgggccgc gccgatgccg
     1501 ccgacccact cgtcaagcgc gcatcccaga tccgacagga tatcggtgcg ctgctcgggc
     1561 ccaaccacgc ctttttcgct tcagaccatg ccaatcgggg cgaccttggc ctggcgcgcg
     1621 gcgactggcc ggcggcgctc gactcctatc gcgaggccat tcggctgttg acgggacagg
     1681 acacctcgca aacggtggtg caatccgtcg tgcaagagga aatcaagcgc taccgcgaaa
     1741 ccttcatcgg cctttgccgt gccgcctggc aaatgcgctc gacgccgggt gccaatgcct
     1801 cctcgttgtt tgaggaaaca ttcgccgccg cgcagctcgc ctgggcgact tcggcagcct
     1861 cggcgttggc aaaaatgagc actcgtatcg gtgccggcag caccccgctc ggccagcgca
     1921 ttcggcgcgt acaggatctt tccgaccgcg tcttgcgcct caacggcgag gacaatgccc
     1981 ttctgagcaa ctggagtgcc gtgcagcgcc aggatgctcg ctacagcgcc gtgcaggagg
     2041 agttccgcgc tgcaagcctc gcccgcggca aggcgacggc cccgacggtc aagcgccaga
     2101 cccagttggt acaagagctt accggtttgg tgcagcgctg cccgcccgga cagaacaaag
     2161 ccggctgcga gggaagcgag gcggaaatca aggcgattag ccagcaa
//
LOCUS       DASTMT010000075         6422 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_5272785, whole genome shotgun sequence.
ACCESSION   DASTMT010000075 DASTMT010000000
VERSION     DASTMT010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 6422)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6422)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6422
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5272785"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..649)
                     /locus_tag="VFR00_01585"
     CDS             complement(<1..649)
                     /locus_tag="VFR00_01585"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEU0157968.1"
                     /translation="MDPLRLSALAHGLMICGLIVIFLYLGRPILEPLVIAALLAFILS
                     PLIRRLRQWGVWRVPSVVLAVVFTLGVLGALGAIIAIQIAQLAEELPTYETNLRGKIR
                     ALGTGKLTSGALERASGTLKDLQEEISKAGAGATSAQKPMLVEVRQPEPRGLETIANL
                     VRPLLSPLAMTALIVLFLIFILLQREDLRDRFLRLAGTADLQRSTAALDDAGTRLS"
     gene            complement(899..1675)
                     /locus_tag="VFR00_01590"
     CDS             complement(899..1675)
                     /locus_tag="VFR00_01590"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007612648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HEU0157969.1"
                     /translation="MVRRIIDISVPLQSDIASDPPGTEPKIAYFDHRQSAPQVCSFFP
                     GLSPADLPDGEGWAVERVDISTHNGTHLDAPWHFASTMDKGKRAITIDEVPLEWCFQP
                     GVKLDFRHFPDGYVASADDVAAELKRIGHTLRPLDIVLVNTSAGQRYGEPDYVPTGCG
                     MGREATLYLTSRGVKITGTDAWSWDAPFVHTARKFAASKDAKLIWEGHKSGREIGYCH
                     IEKLHNLEALPAHGFTVACFPVKIKAASAGWTRAVAILEA"
     gene            2001..2936
                     /locus_tag="VFR00_01595"
     CDS             2001..2936
                     /locus_tag="VFR00_01595"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0157970.1"
                     /translation="MSRPQPLLKLLAFLFVTLGAHAAAAQTKIVVGYTATADFASGFI
                     ALDRGLFKQRGLDVEFKLVPLNSQLPAAVQSGSITLGGTTVSVLLQAVDGGLEHVAVA
                     GSGVTWKGQDSFGAVMRADLPYARPEDFLGKKVGAPGIGAFLQVLFRNWLLDNGVDYK
                     KVTFIEVGFPQMADVLKGGNVDAVVTGEPVMGRIVAGGIGKQVTSFTDNLKGELPIIV
                     YSATRKWAAANPAALKAFQEAIAEGAAIANTDQAAVRASVANYIKMPPEVIAGMKLGR
                     WQAAVTDTGIAGWIDIMKRQDMLRTSIDTKSIIAR"
     gene            2938..3708
                     /locus_tag="VFR00_01600"
     CDS             2938..3708
                     /locus_tag="VFR00_01600"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007592620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0157971.1"
                     /translation="MSLPHPALAFAAVALELGGRAILEDIDFTVARGEFVCIIGASGC
                     GKTTLLRLLAGLVAPSRGKVSLDGRRVAAPSPQVALVFQDYGRALLPWRTATGNVSLA
                     LEAMGVPVAERPQRIAALLAKTGLVGHADKYPSQLSGGMQQRLQIARSLAQDPAVLLM
                     DEPFGALDAMTRQGLQDEVLRIVGERRTTVLFVTHDLDEALYLGDRLIVLAANPGRIA
                     RTLDIALPRPRNQLATRENPEFLRLRRDLFTTMQETGA"
     gene            3705..4469
                     /locus_tag="VFR00_01605"
     CDS             3705..4469
                     /locus_tag="VFR00_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015665339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEU0157972.1"
                     /translation="MKAWRGALVPVLLLALAELAFRARSGPSDSLASPSQAIAALLGT
                     LSDGSLLVGSAETLATLFAGLAIGSLAGLGAGLALGSSDLLHRLTFLSIEMLRPIPSV
                     ALLPLSLLIFGFGYGMEIAVIAFATFWPSLILTQSAVAQIEPRLLEVARLLRLSRWQR
                     ATKIVLPAAAPRLMTALRLAAGVALVVAVTAEIVSNPQGLGHDLMVAQETLRPARTLA
                     LLFWVGLIGWGKNALLLRLERWLFAHRGDVDTRIAT"
     gene            4466..5248
                     /locus_tag="VFR00_01610"
     CDS             4466..5248
                     /locus_tag="VFR00_01610"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0157973.1"
                     /translation="MNVRRLPSSVLGGLGLATLILLWHGLTATGLISKAFLPSPAATF
                     DALLGGLRTGDLKGQLAATTLRMLNGWLFASVAAIALGSLIGISDPVRIYLRPTLAFA
                     RSLPASAVIPVAISLLGLGPGMVVGVVTFGSVWPTLLATVQGFANVEPRLQDVARLLH
                     LTRLQYVSKIGLPNAVPDILAGMRLSLTIALILAVIGEMLSGEEGLGSAVLLAARAFR
                     AAELYAGLILLGLVGLASSYALIWVERRTTRWRQAALSPNLL"
     gene            complement(5307..5702)
                     /locus_tag="VFR00_01615"
     CDS             complement(5307..5702)
                     /locus_tag="VFR00_01615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0157974.1"
                     /translation="MGEPKNPAQAKLRDWPMFRAARVNRKGGGAKLDGKRLPRIAELL
                     VRHFEGTPEEKLAWLKAQSAAGKLDGDDPEELAEAEALLAMLVRLARPKAHKQHLSDL
                     LDQGLEATFPASDPIAVGHFTGAEILRSG"
     gene            6100..>6422
                     /locus_tag="VFR00_01620"
     CDS             6100..>6422
                     /locus_tag="VFR00_01620"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HEU0157975.1"
                     /translation="MFFHKQQPEAEQAPPAPISALPPPNAKLELNTPKERSRSLIDRS
                     ANMIGDLWSDGDVQIDGHLCGNVNCAQLIVGREAAVTGVVIAQEAVIRGKMTGLIRAK
                     RVLLQA"
BASE COUNT         1003 a         2178 c         2100 g         1141 t
ORIGIN      
        1 ggctgagccg cgtgccggca tcgtcgaggg ctgccgtaga gcgctgcagg tccgcagtgc
       61 cggccaggcg caggaagcgg tcgcgcagat cctcgcgctg cagcagaatg aaaatgagaa
      121 agaggacgat caaggccgtc atcgccaagg gcgatagcag cggccgcacc agattggcga
      181 ttgtctcgag cccgcgcggc tccggttggc ggacttcgac cagcatgggc ttttgcgccg
      241 aggtggcccc ggcgcccgcc ttgctgattt cctcctgcag atccttgagc gtgccggagg
      301 cgcgctccag ggcacccgac gtcaacttgc cggtgcccag cgccctgatt tttccgcgca
      361 ggttggtttc ataggtcggc agctcctcgg cgagctgcgc aatttggatg gcaatgatcg
      421 cgccgagcgc gcccagcacg cccagtgtga aaaccaccgc cagcacaacc gacggcacgc
      481 gccaaacgcc ccattgccgc agccggcgga tgagcgggga gagaataaag gcgagcaggg
      541 cggcaatgac cagcggctcg aggatcggcc gccccaaata taagaaaata acaataagcc
      601 cgcagatcat cagaccgtgc gccaaggcgc tgaggcgaag gggatccatc tggcccgccc
      661 gcaccagcag tctgcttccg cgatcttgca tgcttggcca attctttgtc ttgggggttg
      721 aacggcgtct cgggatagaa cggcgccccg ccgccgcacg atcgatctcg acggcacagg
      781 ccgcgctcga aaaacgccaa gcgctcggca atggttcccg cgcccggctg ggcacgccgc
      841 ggcgcgcgcg cgccggttca atccggcgcg gcgtcgcgcg cctgcgcggc cccaggcctc
      901 acgcttcgag gatggcgacg gcgcgggtcc agcccgcgga agcggctttg attttgacgg
      961 ggaagcaggc gaccgtgaac ccgtgcgccg gcagcgcctc gaggttgtgc agcttttcga
     1021 tgtggcagta gccgatctcg cggccggact tgtgaccttc ccagatcaac ttggcatcct
     1081 tgctcgcggc gaatttcctg gccgtgtgca caaagggagc atcccacgac caggcgtccg
     1141 ttcccgtaat cttgacaccg cgcgaggtca aataaagggt cgcttcccgg cccatgccgc
     1201 agcccgtcgg cacgtagtcg ggctcgccgt agcgctgacc cgcggaggta ttgaccagca
     1261 cgatatccag cgggcgcagc gtgtggccaa tgcgcttcag ttcggcggcg acgtcgtcgg
     1321 cgctggcgac atagccgtcc ggaaaatggc ggaagtcgag cttcacgccg ggctggaaac
     1381 accactccag cggcacctca tcgatggtga tggcgcgctt gcccttgtcc atcgtcgagg
     1441 cgaagtgcca gggtgcatca aggtgcgtgc cgttgtgcgt ggagatgtcg acccgctcga
     1501 cggcccatcc ctcgccgtcc ggcaggtcgg ccgggctcag acccgggaag aagctgcaga
     1561 cctgcggggc gctttggcgg tgatcgaagt aggcgatctt cggctcggtc cccggaggat
     1621 cggaggcgat atcgctttgc agcggcactg agatgtcaat aatgcgccgg accatcgctg
     1681 gctcctccgc ggtcttaagt gtcgctcaat ggttgtccgc aaacttggtt ttgtcgataa
     1741 aatattgcca gccgcaaacg ccctcgctag cgtgcggcac aggtcggaag cttgcggggg
     1801 gatgccgatg cagcgcatga cgcgcgggca agcgcgacgc ggcgcaatgc tgcgctggaa
     1861 ggcgcaggca cggcccgcag cgatcggacc ggtggtcgcg ggcatcgccg gcgcgatgct
     1921 gcacatgaag ccccgccacg accgacgccg gcgcggcctc cgcagccaac gcccagtcca
     1981 caaccgctcg aggtcatccg atgtcacggc cgcagccgct tcttaaactg ctcgcattcc
     2041 tcttcgtcac gctgggggcg cacgcggcag cggcgcaaac caaaatcgtg gtcggttaca
     2101 ccgccaccgc cgactttgcc tccggtttca ttgccctcga ccgggggctc ttcaaacagc
     2161 gcggcctcga cgtcgaattc aagctggtgc cgctcaattc gcagctgccc gcggcggtgc
     2221 agtccggctc catcaccttg ggtggcacca ccgtctccgt gctcctgcag gcggtcgatg
     2281 gtgggctcga acacgtggcg gtggccggct cgggggtgac ctggaagggc caggattcct
     2341 tcggcgcggt catgcgcgcc gacctgccct acgccagacc tgaggatttc ctcggcaaga
     2401 aggtcggcgc gcccgggatc ggtgccttcc tgcaggtgct gttccgcaac tggctgctcg
     2461 acaatggcgt cgactataag aaagtgacct tcatcgaggt cggcttcccg cagatggccg
     2521 acgtcctgaa gggcggcaat gtcgatgcgg tggtcaccgg cgaaccggtg atgggccgca
     2581 tcgtggcggg gggcatcggc aaacaggtga cgagctttac cgacaacctg aaaggcgagc
     2641 tgccgatcat cgtctattcc gccacccgca agtgggccgc ggccaatccc gcggccctca
     2701 aggcctttca ggaggccatc gccgagggtg cggccatcgc caataccgat caggcggcgg
     2761 tacgggcctc cgtcgccaac tacatcaaga tgccaccgga ggtcattgcc ggcatgaagc
     2821 tcggacgctg gcaagcggcg gtgaccgaca ccggcatcgc cggttggatc gatatcatga
     2881 agcggcagga tatgctgcgc accagcatcg acaccaaaag catcatcgcg cgttagcatg
     2941 tcgctgcccc accccgccct cgcgttcgca gccgtggcgc tcgaactcgg cgggcgggcg
     3001 atcctcgagg acattgattt taccgtcgcg cgcggcgagt tcgtctgcat catcggtgcg
     3061 tcgggctgcg gcaagactac cttgttgcgc ctcttggccg gtctcgtcgc accaagccga
     3121 ggcaaagtca gcttggacgg ccgccgcgtg gcggcgccgt cgccgcaagt ggccttggtc
     3181 ttccaggact atggacgcgc gctgctgccc tggcgcaccg cgacgggcaa tgtcagcctg
     3241 gcgttggagg cgatgggcgt gcccgtcgcc gagcggccgc agcgcatcgc cgccttgctc
     3301 gccaagaccg gtctcgtcgg ccatgccgac aagtatccga gccaactttc cggcggcatg
     3361 cagcagcgtc tgcagattgc ccgcagcctg gcccaggatc ccgccgtgct gctgatggac
     3421 gagccgttcg gcgcgctcga tgccatgacg cgccagggct tgcaggacga ggtgctgcgc
     3481 atcgtcggcg agcgccgcac aaccgtgctg ttcgtcaccc atgacctgga cgaggcgctc
     3541 tatctgggtg accgcctgat cgtgcttgct gccaatcccg ggcgcatcgc gcgcacactc
     3601 gacattgcct tgccgcggcc gcgcaatcag cttgccaccc gcgaaaatcc ggagttcctg
     3661 cgcctgcgcc gcgatctttt cacaaccatg caggagacgg gggcatgaaa gcgtggcgcg
     3721 gcgcgctcgt ccccgtgctg ctgctggcac tcgccgagct cgcctttcgc gcccgcagcg
     3781 ggccaagcga cagcctggcg tccccttcgc aggccatcgc ggccctgctg gggacgctga
     3841 gcgatggttc gctgctggtc ggcagcgctg agaccttggc aacgctcttc gccggcttgg
     3901 cgatcggcag ccttgccggt ctcggtgccg gtctggcgct gggatcctcc gatctgctgc
     3961 accggcttac ctttttgagc atcgagatgc tgcggccgat cccatcggtg gcgctgttgc
     4021 cgctttcgct gctgatcttc ggctttggct acggcatgga gatcgccgtc attgcctttg
     4081 ccaccttctg gccgtccctg atcctgacgc agagcgcagt cgcccagatc gagccgcgtc
     4141 tgctcgaggt ggcgcgtttg ctgcgactga gccgatggca gagagccacc aagatcgtgc
     4201 tgccggcggc cgcgccgcgc ctgatgacgg cgctgcggtt ggcggctggc gtcgccctgg
     4261 tggtggccgt gaccgccgaa atcgtcagca atccgcaagg tctcggacac gatttgatgg
     4321 tagcgcagga gaccttgcgc ccggcgcgca ccttggccct tctgttctgg gtcggtctga
     4381 tcggctgggg caagaacgcc ctgctgttgc gcctcgaacg ctggttgttc gcgcaccgcg
     4441 gcgatgtcga taccaggatc gccacatgaa cgtgcgccgc ttgccgagct ccgtcctggg
     4501 tggcttggga cttgcaacgc tcattttgct ttggcacggc ctcaccgcca cggggctgat
     4561 ctcgaaggcc ttcctgccct cgccggctgc aacgttcgac gccctgctcg gcggccttcg
     4621 cacgggtgat ctcaagggcc agctcgcggc caccaccttg cgcatgctca atggctggct
     4681 gttcgcaagc gtcgcggcca ttgcgctcgg cagcctgatc ggcatctccg acccggtgcg
     4741 gatctatctg cgccccacgc tcgcctttgc gcgctcgctg ccggcttctg ccgtcattcc
     4801 cgttgccatt tccctgctcg ggctcggccc cggcatggtg gtgggcgtcg tcaccttcgg
     4861 gtcggtgtgg ccgacgctgc tcgccacggt gcaaggcttt gccaacgtcg agccgcgctt
     4921 gcaggatgtg gcccggctgc tgcacctaac gcgcctgcaa tacgtaagca aaatcggtct
     4981 gcccaacgcc gtgcccgaca ttttggccgg catgcgcctg tcgctgacca tcgccctgat
     5041 tttggccgtc atcggcgaga tgctgtcggg cgaggaaggt ctcggcagcg ccgtcctgct
     5101 ggcggcgcgg gcgtttcgcg ccgccgagct ttacgccggg ctgatcctgc tcggcctcgt
     5161 cgggcttgcc agcagttacg ctctcatttg ggtggaacgg cgcaccacgc gctggcgcca
     5221 agcggccctg tcgccgaacc tgctctgagc gcctctcgcc gccgcggacg caaaggccgc
     5281 ggtgcgcatc gcccacctgc ggcgcctcag ccggagcgca ggatctccgc gccggtgaaa
     5341 tgaccgaccg cgatcgggtc gctggctgga aaggtcgctt ccaatccctg atcgagcaag
     5401 tcgctcaagt gctgcttgtg ggctttgggg cgcgccaggc ggaccagcat cgccagcaaa
     5461 gcctcggcct ccgcgagctc ctcggggtcg tcgccgtcaa gcttgcccgc cgcgctttgc
     5521 gccttgagcc aggccagctt ttcctcgggc gtaccctcga agtgcctgac caaaagctcg
     5581 gcaatccgag gcaggcgctt gccatcgagc ttcgcgcccc cgcctttgcg gttcacccgc
     5641 gccgctcgaa acatcggcca gtcccgcagc ttcgcctgcg cggggttctt cggttcgccc
     5701 acgacgcctc caacacacgc ttgctcggtg agccgctgat ggacgtcggc tgagcttagc
     5761 cgagatttgc cgcccggcct atgctctagc gtgcgcctcc ttcgcgggcg caacagggca
     5821 tggcccgccg ccgacggctg cgggccgcgg cggacgactg ggccggccct tacacaatga
     5881 tcattcccca aaatacagcc tcccagcagg tgttgggccc gagcttgccg tctacaccct
     5941 gcccccagca cgcgcagagc cggcgtcctc gccgcgtccc gcggcaggat cccaatcgcc
     6001 cgctcgtggc tggtttgccc gagcgaacgg atccgccggg tcagcgttgt tatggaagtt
     6061 catggccctt gggcgcaaac ggcacgggaa ggtcgccaga tgtttttcca taagcagcag
     6121 cccgaggcag agcaggcgcc gcccgcgccg atctcagcgc tgccgccgcc caatgcaaag
     6181 ctggaattga atacgcccaa ggagcggtcc cgctctctga tcgatcgctc ggcgaacatg
     6241 atcggcgacc tgtggagcga cggcgacgtc cagatcgatg ggcacctctg tggcaatgtc
     6301 aactgtgcgc agttgatcgt cggccgggag gcggccgtca ccggcgtcgt catcgcgcag
     6361 gaggccgtca tccgcggcaa gatgacgggc ctgatccgcg ccaagcgcgt ccttttgcag
     6421 gc
//
LOCUS       DASTMT010000076         3158 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_2011356, whole genome shotgun sequence.
ACCESSION   DASTMT010000076 DASTMT010000000
VERSION     DASTMT010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3158)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3158)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3158
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2011356"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2529
                     /locus_tag="VFR00_01625"
     CDS             <1..2529
                     /locus_tag="VFR00_01625"
                     /inference="COORDINATES: protein motif:HMM:NF017081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEU0157976.1"
                     /translation="IAATFAASLTVDRSLASSLAFGLCNAGEALLAMWLIGRWFGPTF
                     SLDSLRGVLGFFAASSLAVAVSATVASTAMQLFGPAEAKFLEVWKVWFAADVLGAVTV
                     APLLIGVVAAARDVPPWRELIEGNLAVVVIAVTNGFALALLAGSSSLIEPGSFLFPLL
                     LWLGARCRPVFAAAAVFTIAAGIVWTTIHEFGRYGDPNEALTDRVFAAQVAMLGTTLA
                     ALALAAIFAERRRHQAAQAESDRRLRSSLDAANVIAWDVDLERNTVHSAGPVRRLLDL
                     PEGALPQDFAAMVDTIHPLDRERVMRQFWQAVGTAAVYRLEFRLNVTSDLRWVTAEGS
                     IGRDADGRPVRVRGITHDISERKKAELALAERDAQLQLAERAARVGSFAIDIATGNVQ
                     ISPGYATIHGLGDGTDEFARADWRTRVQADDLARLDALRTQAFAEQRREHNTEYRIVL
                     AEGKTRWIESRGLLSYADDGRPTRLVGVNIDITERKRAAAALQESEARYRALYDDNPS
                     MYFTVDVDGIVLSVNAFGAQQLGYTAAELVGRSVLQVVHPEDREVALRHLARCAEQHE
                     TVVTTELRKVHRNGSIMWVREVARAVRDRGLLIVCEEITERKRAEDQQSLLIAELDHR
                     VKNVLASVAAIARRTGERKGSMEDFVDRLDRRIQSMADAHDLLSRNRWQGVSLAELVN
                     RELAPYAGDGNTLVTGPDIVLAAAATQAMAMVLHELATNAAKYGALSSPQGRVCVRWQ
                     RQAPDGAPTKLRLEWLEEGGPRVVAPAEAGYGTSVIRDLIPYELGGAVELNFGPSGVA
                     CVIEISVAHDVVRLVNPSTDFEVLRAAGAEAAEPAPLKRQLRPT"
     gene            complement(2586..3056)
                     /locus_tag="VFR00_01630"
     CDS             complement(2586..3056)
                     /locus_tag="VFR00_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008969388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEU0157977.1"
                     /translation="MDAFDRKLLAALQDDGRLTNEELADRVGLSSSQCSRRRGALEAA
                     GIIESYHAHLSAEALGLGVVVFVAVTLATHSPDTSKRFRRLIDRLDEVQEAYALTGTA
                     DYLVKLVVPDLKALASILNDVFLPHASIQHVHSSIVLDCLKQTSNLPLGHIPRL"
BASE COUNT          508 a         1009 c         1078 g          563 t
ORIGIN      
        1 atcgcggcaa cctttgccgc cagtctcacc gtcgacagga gtttagcgag ctcccttgcg
       61 ttcggtttgt gcaacgccgg cgaggcgctg ttggcgatgt ggttgattgg gcgctggttc
      121 ggtcccacct tcagtctcga cagcctgcgc ggcgtgcttg gcttttttgc cgcgagcagc
      181 ttggcggtgg cggtctccgc caccgttgcc tcgacggcga tgcaactgtt tggccccgcc
      241 gaggccaagt ttctcgaggt ctggaaggtt tggtttgccg ccgatgtgct tggcgccgtc
      301 acggttgcgc cgctgttgat cggcgtggtg gcagccgcac gcgatgtgcc gccctggcgc
      361 gagctcattg aaggcaacct cgccgtcgtc gtgatcgccg tgacgaacgg ttttgccttg
      421 gctctgctgg cgggttcgtc ctcgctgatc gagccgggca gtttcttgtt tccgctgttg
      481 ctgtggctcg gcgcccgctg ccgtcccgtc tttgccgcgg cggccgtctt cacgattgct
      541 gccggtatcg tctggaccac cattcacgag tttggccgct acggtgaccc gaacgaggcg
      601 ctcaccgacc gggtgttcgc ggcccaggtt gccatgctcg gcaccaccct ggcagcgctc
      661 gccctggccg cgatctttgc cgagcgacgt cggcatcaag cggcgcaggc ggaaagcgac
      721 aggaggctgc gatcgagcct ggatgcggcc aacgtcatcg cttgggacgt ggatcttgag
      781 cgcaataccg tgcattcggc gggcccggtc agacgtcttc tcgatctgcc cgagggcgcc
      841 ttgccacagg actttgccgc catggttgac accattcacc cgttggaccg cgagcgggtg
      901 atgcgccaat tctggcaagc cgtcggcacg gccgccgtct accgcttgga gtttcgcctc
      961 aacgtcacgt ctgacctgcg ctgggttacg gccgagggct caatcgggcg cgatgccgac
     1021 ggccggccgg tgcgggtacg cggcatcacg catgacatca gcgagcgcaa gaaagccgaa
     1081 ctggcgctgg ccgaacgcga tgcccagctg cagctcgccg agcgggcggc ccgcgtcggc
     1141 agcttcgcca tcgatattgc gaccggcaat gtgcagatct cacctggcta cgccaccatc
     1201 cacggcttgg gcgatggcac cgacgagttt gcgcgggcgg actggcgcac gcgcgtgcag
     1261 gccgatgatc tcgcccggct cgatgcgctc cgcacccaag ccttcgccga gcagcgccgt
     1321 gagcacaaca cggagtaccg catcgtgctc gccgagggca agacgcggtg gatcgaatcg
     1381 cgcggcctgc tgtcctacgc tgatgacggg cggccgaccc gcctcgtcgg cgtcaacatc
     1441 gatatcaccg agcgcaagcg tgcggcggct gccctgcagg agagcgaggc gcgctaccgt
     1501 gcactctatg atgacaatcc ctccatgtac ttcaccgtcg acgtcgacgg catcgtgctc
     1561 tcggtcaatg ccttcggcgc ccaacagctg ggctacacag cggcggagct ggtgggacgc
     1621 tcggtactgc aggtggtgca cccggaggac cgggaggtgg cgctgcggca cctggcgcgc
     1681 tgcgccgaac agcacgaaac cgtcgtcacc acggaattgc gcaaggttca tcgcaacggc
     1741 agcatcatgt gggtcagaga ggtagcccgc gccgtgcgcg ataggggcct gctcatcgtt
     1801 tgtgaggaga tcaccgagcg caagcgcgcg gaggatcagc agagcttgct gatcgccgag
     1861 ctcgaccacc gcgtcaaaaa tgtgctggcc agtgtcgccg cgatcgccag gcgcacgggc
     1921 gagcgcaagg gctcgatgga ggatttcgtc gatcggctgg accgtcgcat ccagtcgatg
     1981 gcggacgcgc acgatttgct gagccgcaat cgctggcagg gggtcagctt ggccgagctg
     2041 gtcaatcgcg agctggcgcc ctacgccggc gacggcaata ccctggtgac cggtcccgac
     2101 atcgtgctgg cggcggcggc gacccaggcc atggccatgg tgttgcacga gcttgccacc
     2161 aatgccgcca aatacggggc gctgtcgagc ccgcaaggcc gcgtgtgcgt gcgctggcag
     2221 cggcaggcgc cggacggcgc gccgaccaag ctcaggctgg aatggctgga agagggcggg
     2281 cccagagtgg ttgccccggc cgaggccggc tacggtacca gtgtcatccg cgatctcatc
     2341 ccctacgagc tgggaggcgc cgtcgagctc aacttcggcc cgagcggcgt cgcctgcgtc
     2401 atcgaaattt cagtagccca cgacgtcgtg cgcttggtca atcccagcac cgactttgag
     2461 gtcttgcgcg ccgccggcgc cgaggcggcc gagcctgcac ctcttaagcg ccagttaagg
     2521 cccacctgat caccggtgcc gggctcgcgg cgcggaccgc gctggatggg ccatcctgca
     2581 ggatgttaga ggcgggggat gtggcccaag ggcaggttcg aggtctgctt caggcaatcg
     2641 agcacgattg atgagtgcac gtgctggatg gaggcgtgcg gcaggaacac atcgttgagg
     2701 atgctggcaa gcgccttgag gtccggcacg accagcttga cgaggtagtc cgccgttccc
     2761 gtcaaggcat aggcctcctg cacctcgtcg aggcgatcga tcaggcgtcg aaagcgtttc
     2821 gaggtgtcgg gtgaatgggt ggcgagcgtg accgccacga acaccaccac gcccagtcca
     2881 agcgcctcgg ccgacaagtg cgcatggtag ctttcgatga tgccggccgc ctcgagcgcg
     2941 ccgcgtcggc gcgagcattg cgaggaggaa agcccgaccc ggtcggcgag ctcttcattg
     3001 gtcaggcggc cgtcgtcctg cagggcggcc agcaattttc gatcgaaggc gtccatgcac
     3061 gattcccgca gctttatggg taaagtcgca cgatatgtgc aatcatgacc agcaagcgcg
     3121 gctttatgca agcaccgtgc gcggcgccag cggtatga
//
LOCUS       DASTMT010000077         2148 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_371921, whole genome shotgun sequence.
ACCESSION   DASTMT010000077 DASTMT010000000
VERSION     DASTMT010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2148)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2148)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2148
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_371921"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            302..754
                     /locus_tag="VFR00_01635"
     CDS             302..754
                     /locus_tag="VFR00_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006611967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEU0157978.1"
                     /translation="MARLTVNGKTHDIDVDPDTPLLWAIRDHVGLTGTKYGCGIAQCG
                     ACTVHIDGEAVRSCSMPVSAVEGKAITTIEGLAPNGALHKVQQAWIDNDVPQCGYCQC
                     GMIMAVAALLKEKPKPTDADIDNAITNICRCGTFQQVREAIHMAAQSA"
     gene            766..>2148
                     /locus_tag="VFR00_01640"
     CDS             766..>2148
                     /locus_tag="VFR00_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018268014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEU0157979.1"
                     /translation="MTSMKLDRRSFVVGAAAAAGGGMALSVGFPFVGPQVVRAQDGSP
                     EVNAWVVIRPDDTVVIRIARSEMGQGTLTGLAQLVAEELECDWSKVTTEYPTPGQSVA
                     RKRVWGDFSTGGSRGIRNSHLYVRQGGAIARTMLLQAAANDWKVPVGELTVANSIITH
                     AASGRKTSYGKVAEAAAKLEAPKEVPLKDPKDWKIAGKPLKRLDTADKVVGKQVYGAD
                     LKLPGMLTATIKDCPVTGGKVASFDAAKVAGMKGVKKVVQVGDSAVAVVADTFWQAKT
                     ALDALPIVWDEGENKKVSSATIAQWLKEGLDAPEAFVGNENGDVKAAIAKAAKKVEAV
                     YSYPFQNHAPMEPMNATALYTPDKCEVWCPTQNGEAAFAATVAAAELPASKVEVYKLH
                     LGGGFGRRGAVHDYVRQAVLIAKQMPGTPVKLLWTREEDMLHGRYHPVMQAKLVGAFD
                     ADNNLTGIHVR"
BASE COUNT          411 a          684 c          723 g          330 t
ORIGIN      
        1 tcggccagat cggcaagacc ggcctgatcc cggccggtct ggtcaccggg cgcaggctct
       61 gttcgcgact gcacgcgggc tctgtgcgct gcagcgagcc tgacctcgcg accttttcgg
      121 ttgccgttga gaacacacgc ttaggatgag agggcgcatc gacgttgcgc gccccgtcgc
      181 cttggcctcg gcggtctcac cttcgcgtga gagcggagct agcctcttat actttagtcg
      241 ccacatcggc taagctcgcg cgcaggacgt gagcacccgc ttgcaggagg aggaaaccct
      301 gatggcgcgc ctgaccgtta atggcaaaac gcacgatatt gatgttgacc ccgatacccc
      361 gctgctgtgg gcgattcggg accatgttgg gctaacgggg accaagtatg ggtgcggcat
      421 cgcccagtgc ggcgcctgca ccgtgcacat cgacggggag gcggtgcgct cctgttcgat
      481 gcccgtcagc gccgtcgaag gcaaggccat caccaccatc gagggcttgg ctccgaacgg
      541 tgccctgcac aaggtgcagc aggcctggat cgacaacgac gtgccccagt gcggctactg
      601 tcagtgcggc atgatcatgg ccgtggcggc cctcctgaag gagaaaccca agcccacgga
      661 cgccgacatc gacaatgcca tcaccaatat ctgtcgctgc ggcaccttcc agcaggtgcg
      721 cgaggcgatc cacatggccg ctcagagcgc gtaagggagg cagccatgac aagcatgaaa
      781 ctcgatcgtc gctccttcgt ggtcggtgct gcggccgccg ccggcggcgg catggcgctc
      841 agcgtcggct ttccctttgt cggcccacag gtggtcaggg ctcaggacgg ttcacccgag
      901 gtcaacgcct gggtggtgat caggcccgat gacaccgtcg tcattcgtat cgcgcgctcg
      961 gagatggggc aggggacctt gacgggtttg gcccagctcg tggccgagga gctcgagtgc
     1021 gactggtcga aggtgacgac ggaatatccc acgcccggcc agagcgtcgc gcgcaagcgc
     1081 gtgtgggggg acttctccac cggcgggagc cgcggcatcc gcaactcgca cctctacgtg
     1141 cgccagggcg gggccatcgc ccgcaccatg ctgctgcagg cggccgccaa cgactggaag
     1201 gtgccggtcg gcgaattgac cgtcgccaac agcatcatca cgcatgccgc atcggggcgc
     1261 aaaaccagct acggcaaggt ggcggaggcc gccgccaagc tcgaggcgcc caaggaggtg
     1321 ccgctcaagg accccaaaga ctggaagatc gccggcaaac cgctgaagcg cctcgatacc
     1381 gccgacaagg tggtcggcaa gcaggtctat ggtgccgatc tcaagctgcc gggcatgctg
     1441 acggccacca tcaaggactg ccccgtcacc ggcggcaagg tggcgagctt cgatgccgcc
     1501 aaggtcgcgg gcatgaaggg cgtcaaaaag gtcgtgcagg tcggcgacag cgccgtggcg
     1561 gtggtggccg ataccttctg gcaggccaag acggcgctcg atgcgctgcc gatcgtgtgg
     1621 gacgagggcg aaaacaaaaa ggtctcgagc gcgaccatcg cccagtggct caaggaaggg
     1681 ctcgacgcgc cggaagcttt cgtcggcaac gaaaacggcg acgtcaaagc ggcaatcgcc
     1741 aaggcggcaa aaaaagtgga ggcggtctac agctatccct tccaaaacca cgcgccgatg
     1801 gagcccatga acgccacggc gctctacacg ccagacaagt gcgaggtgtg gtgtccgacc
     1861 cagaacggcg aggcggcttt cgccgccacc gtggcggccg ccgaactgcc ggccagcaag
     1921 gtcgaagtct ataagctcca cctgggcggc ggtttcggcc gacgcggggc cgtgcatgac
     1981 tacgtgcggc aggccgtgct gatcgccaag caaatgccgg gcacgcccgt gaagctgttg
     2041 tggacgcgcg aagaggacat gctgcacggc cgctaccatc cggtcatgca ggccaagctc
     2101 gtgggtgcgt tcgatgccga caacaatctg accggcatcc atgtgcgc
//
LOCUS       DASTMT010000078         2120 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_1682531, whole genome shotgun sequence.
ACCESSION   DASTMT010000078 DASTMT010000000
VERSION     DASTMT010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2120)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2120
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1682531"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1525
                     /locus_tag="VFR00_01645"
     CDS             <1..1525
                     /locus_tag="VFR00_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEU0157980.1"
                     /translation="SLGLLGWIGFKLNLFLNVMTPLVMVMGFADSMQMVSAIRIRLRE
                     GDSKGEAVRFAVRIVGPACVLAHGTALLSFVALLFSPSGLIRTFGMAGALAVCISFIA
                     VLLVLPLLGLILIRNEASLARDRSPADRLMDGLGGLVGWIVDRVVRWPFTTTLVALAS
                     FLLLGHAYLQLEPRYRLADQVPDREQALGATERIDQKLTGANPVHIMIEWQTGESIYA
                     PGPLSVIAAAHQVLEKQAGLGNVWSLESLRRWLKEAGDDRPETIKKYVGYLPEHLVRR
                     FIAKEGNAVLVTGRLPDVDSSRILPVVEKIDHALDQVRAEHPQFRLSVTGLPAIAARN
                     SARMISQLNESLPICVAFAALLLALAFRSVFVGLVSLLPGLYPVVTSGAIIQWTGGGL
                     EFASVVALLVVFGLAIDALIHFFNRLSLEERVEPRPEVAIRNARVLVGPAIILTTLVL
                     AFGLGVTVFSDLPSLRTFGFVCGTTLLASLIADLIFLPGLIMLVRKIWRVRRPTPAG"
     gene            1614..>2120
                     /locus_tag="VFR00_01650"
     CDS             1614..>2120
                     /locus_tag="VFR00_01650"
                     /inference="COORDINATES: protein motif:HMM:NF013384.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-eliminating lyase-related protein"
                     /protein_id="HEU0157981.1"
                     /translation="MAQQFASDNNAGICPEALEALLRANGEGHVAGYGDDAWTAKACD
                     CLRELFETDCEVFFVFNGTAANALALAQLARPYHAIIAHRLSHIEEDEAGAPGYLSGG
                     AKIVALDLPLAKLTPAAVRASANAGRGVHHVKPRVLSLTEATELGTVYDVDEVGALCE
                     TAHGCDLKV"
BASE COUNT          311 a          699 c          698 g          412 t
ORIGIN      
        1 gtcgctcggg ctgttgggtt ggatcggctt caaactcaat ctgtttctca atgtgatgac
       61 gccgctcgtc atggtgatgg gctttgccga cagcatgcaa atggtatcgg cgatccgcat
      121 ccgtctgcgc gaaggtgaca gcaagggtga ggccgtccgc ttcgccgtgc gtatcgtcgg
      181 cccggcgtgc gtgctggcgc acggcaccgc gctgctgtcc ttcgtggcgc tcttgttctc
      241 gccctcgggc ctgatccgga ccttcggcat ggccggggcc ttggcagtct gcatctcttt
      301 cattgccgtg ctgctggtgc tgccgttgct cggcctcatc ctgatccgca acgaggcctc
      361 gctggcgcgg gacaggtcgc ccgccgaccg gctcatggat ggccttgggg gcttggtggg
      421 ttggatcgtc gaccgcgtcg tgcgctggcc tttcaccacg acgctggtcg cgctcgcatc
      481 gtttttgctg ctcggccacg cctacctgca acttgagccg cgctaccggc tggcggacca
      541 ggtgcccgat cgggagcagg cgctcggcgc caccgagcgc atcgatcaaa aactgaccgg
      601 cgccaacccc gtgcacatca tgatcgaatg gcagaccggc gaatcgatct acgccccggg
      661 tcccctcagc gtgatcgccg ccgcccacca ggtgctggag aaacaggccg gcctcggcaa
      721 cgtctggtcg ctcgaaagcc tgcgccgctg gctcaaggaa gcaggcgacg accgccccga
      781 aaccatcaag aaatacgtgg gctatctgcc ggagcacctg gtgcgccgct ttattgccaa
      841 agagggcaac gccgtgctgg tgaccggtcg gctgccggac gtcgattcga gccgcatcct
      901 accggtggtg gaaaagatcg accacgcgct cgatcaggtg cgggctgagc acccgcagtt
      961 ccgtctgtcg gtcaccggcc tgccggcgat tgccgcccgc aacagcgcgc gcatgatctc
     1021 ccagctcaac gaaagcctgc cgatctgcgt cgccttcgcc gcactgctgc tggcgctggc
     1081 atttcgctcc gtgttcgtgg gattggtcag tctgctgccc ggcctttacc ccgtagtgac
     1141 ctcgggcgcg atcatccaat ggacgggcgg cggcctcgaa ttcgcctccg tcgtggcgct
     1201 gctggtcgtg ttcgggctgg ccatcgatgc cttgatccat tttttcaacc ggctgagcct
     1261 ggaagagcgt gtcgaaccgc ggccggaggt ggcgatccgc aatgcccgcg tcctggtcgg
     1321 cccagccatt attctgacca cgcttgtgct ggccttcggg ctcggcgtga cggtgttctc
     1381 ggacctaccc agcctgcgca ccttcggctt cgtgtgtggc accacgctgt tggcctcgct
     1441 tatcgccgac ctcatcttcc tgccggggct gatcatgctg gtgcgcaaaa tctggcgggt
     1501 gcgccgcccg acgccggcgg gctaaggggg cttcgccgca cctggcaagg acccgcgtga
     1561 cccggcggcg cctgcgccct atggtttggc ctcggccaca gctggagacc gcgatggcgc
     1621 aacaattcgc ctcggacaac aatgccggca tctgccccga agcgctcgag gccctgctca
     1681 gagccaatgg cgaggggcac gtggccgggt acggcgatga tgcctggacc gccaaggcct
     1741 gcgattgtct gcgtgagctg ttcgagaccg actgcgaggt gtttttcgtc tttaacggca
     1801 cggcggccaa cgcgctggcg ttggcgcagt tggcgcgacc ctaccacgcc attattgcgc
     1861 accggctgag ccacatcgag gaggatgagg cgggcgcgcc cggatacttg agcggcggcg
     1921 ccaagatcgt ggcgttggat ctgccgcttg ccaagctgac gcctgcggcc gtgcgggcaa
     1981 gcgccaacgc tggtcgtggc gtgcaccacg tcaagccgcg cgttctgtcg ctgaccgagg
     2041 cgacggaact tggcaccgtc tacgacgtcg atgaggtcgg cgccctgtgc gaaacggcgc
     2101 atggctgtga tctcaaggtg
//
LOCUS       DASTMT010000079         2357 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_373925, whole genome shotgun sequence.
ACCESSION   DASTMT010000079 DASTMT010000000
VERSION     DASTMT010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2357)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2357
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_373925"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..671
                     /locus_tag="VFR00_01655"
     CDS             <1..671
                     /locus_tag="VFR00_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0157982.1"
                     /translation="VAGDRKPYIQIDRIEGLIAAAQIAGIEYHPWNNQPGRPSVPGRL
                     IFDLDPAPEVSFDRVVAAANELRERLEALGLVTFCKTTGGKGLHVVTPLSAQENDPLG
                     WDEAKAFAEAVCAAMAADSPSRYLLNMAKKLRAGRILLDYLRNDRMSTAVAPLSPRMR
                     AGAPVSMPLPWSQVRPGLAPQRFTMMTAPALLLTSKAWADYSVAGAPLKVAIRRLVGI
                     HKGR"
     gene            complement(801..1262)
                     /locus_tag="VFR00_01660"
     CDS             complement(801..1262)
                     /locus_tag="VFR00_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015319209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEU0157983.1"
                     /translation="MLLGVISDTHGLLRPEAIDALYGSAMIIHAGDVGRVAVLERLRG
                     IASVIAVRGNIDTEPWAATLPETAIVAAGGHKILVLHDLSQLAFDPAEAGLAAVVFGH
                     SHKPSMEKRAGILYLNPGSAGPRRFRLPTTLARLAVSPAGLEPELITLAVS"
     gene            complement(1269..2111)
                     /locus_tag="VFR00_01665"
     CDS             complement(1269..2111)
                     /locus_tag="VFR00_01665"
                     /inference="COORDINATES: protein motif:HMM:NF014759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HEU0157984.1"
                     /translation="MRRPLLIVDGDSFAHRAYHALPKSIRRRGNKGGGAILGFANLLL
                     RLHQAENPRAVLVGWDTLAEPTYRHQALADYQSGRTFDDELTDQLEVLPQFVASLGFA
                     CAKAAGYEADDFLAAATAREEKLGGTALVASGDRDAYQLASERTTILQPIRAGEMARI
                     GPAEVRQRYGVEPKQVPDFIALRGDSSDRIAGAAGVGPVGAAGLLRKYGTLEGVLAAG
                     RFAAQADKLRLYRAIATMDKTAPLPPLRNQKVTWAKASLLAREWQLNKLADRLQALAT
                     KGSK"
BASE COUNT          429 a          743 c          786 g          399 t
ORIGIN      
        1 gagtggcggg cgatcgcaag ccctatatcc agattgaccg aattgaaggc ttgattgccg
       61 ctgcgcaaat cgccggcatt gagtatcacc catggaacaa ccagcctggt cgaccgagtg
      121 ttccgggccg gctgattttc gatctcgatc cggcgcccga ggtcagcttc gatcgtgtgg
      181 tcgctgcggc gaacgagctg cgtgaacggc tagaggctct cgggcttgtc accttctgca
      241 aaaccacggg tggcaaggga ctgcacgtgg tgacaccgct cagcgcgcaa gaaaacgatc
      301 cgctgggctg ggatgaagcc aaggcatttg ccgaggcggt ctgcgcggcc atggccgccg
      361 acagcccgag ccgatacctt ctcaacatgg cgaagaagtt gcgggccgga cgcatccttc
      421 tcgactatct gcgcaacgac cgcatgtcga ccgcggtcgc gcccctgtcg ccacgcatgc
      481 gggcgggtgc accggtctca atgcccctcc cctggagcca agtgcggccg gggctcgcgc
      541 cgcagcgctt caccatgatg acggctccag cccttttgct gacaagcaaa gcatgggccg
      601 actacagcgt tgcgggggcg ccccttaagg tggcgatccg caggctggtc ggcatccaca
      661 agggacgctg agtgtcgggc tggcttgcca acgacctggc aggcattggc ggtggcaacg
      721 tcggcggctc gcagcgtggg cggtaacatg cgggcggccg gaaggcacct tggcgcggcc
      781 ggacgccctg tcatcaagtc tcaggatacg gccagggtaa tgagctcggg ttcgaggccc
      841 gcgggcgaca cggcgaggcg ggccagcgtc gttggcaggc gaaagcggcg tggacccgca
      901 ctgccggggt tcaaataaag gatgcccgcg cgcttctcca ttgagggctt gtgcgaatgc
      961 ccgaatacga ccgccgctag ccccgcctca gcggggtcaa acgcgagttg agacagatcg
     1021 tggagcacca agatcttatg cccgcccgcc gctacgatgg cagtttcggg cagcgtggca
     1081 gcccaaggct cagtgtcgat gttgccgcgc acggcaatga cggaagcaat gccgcgcaat
     1141 cgctcgagaa cggcgactct tcccacatcg ccggcatgga taatcatggc agagccgtac
     1201 agcgcatcga tcgcctcggg gcggagcaga ccatgcgtat cggagatgac gccgagcagc
     1261 atgagacctc actttgagcc cttcgtcgcc agtgcctgca agcggtcggc cagcttgttg
     1321 agttgccatt ctcgggcgag tagcgacgct ttggcccaag tgaccttttg attgcgcagc
     1381 ggcggcaagg gcgccgtctt gtccatggtg gcaatggcgc gatagaggcg aagtttgtcg
     1441 gcctgggccg cgaaccgtcc cgcggcaagg accccctcaa gcgtaccgta cttgcgcaat
     1501 agcccggccg cgccgacggg gcccacgccc gcggcaccgg ctatccggtc cgaggaatca
     1561 ccgcgcagtg caataaagtc ggggacctgc ttgggctcga cgccgtagcg ctgacgaacc
     1621 tcggcgggac cgatccgagc catttccccg gcgcggatcg gctgcaggat ggtcgtgcgc
     1681 tcggaggcga gttgataggc gtcgcggtcg ccgcttgcca ccagcgcggt gccgccaagt
     1741 ttttcctcgc gggccgtggc tgccgccagg aaatcgtcgg cttcatagcc cgcggccttg
     1801 gcacaggcga agcccaacga tgcaacaaac tgcggcagca cctccagctg gtcggtcaat
     1861 tcgtcgtcga aggtgcggcc cgactgatag tccgccaggg cctggtggcg ataggttggc
     1921 tcagcgagcg tgtcccagcc aaccaggacc gctcgcgggt tctcggcctg gtgcaggcgt
     1981 aggagaagat tggcaaagcc aagaatggcg ccgccaccct tgttgccccg ccgccggatc
     2041 gacttcggca gagcatggta ggcgcgatgc gcaaaggagt cgccatcgac tatgaggagc
     2101 ggtcgcctca tgcctcacca cttgggcagc gcaagatttg gcacagcaac gccagcaaga
     2161 tcagcaagct tgcgatcgat ttgtaaaggc ggaaaggctc cccctcaagt cgtgacatgc
     2221 gccagccaat gcgcgatggc gccgggcctt caatgagcgt gagccggcgt gaacgggcct
     2281 agcctaccgc tgacgccgcc ttttttcgct ttcgcgcgtt accgcctcgg ccggtttgtc
     2341 gtcgcgcagg ccgagga
//
LOCUS       DASTMT010000080         1978 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_2998616, whole genome shotgun sequence.
ACCESSION   DASTMT010000080 DASTMT010000000
VERSION     DASTMT010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 1978)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1978)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1978
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2998616"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..375
                     /locus_tag="VFR00_01670"
     CDS             <1..375
                     /locus_tag="VFR00_01670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEU0157985.1"
                     /translation="YSHGCMRVQNPGKLAEILLGEDKGWSPEQVRDLMARGGNNEITL
                     STQIPVHVTYFTAVAGDDTHVKFHADLYGHDRRLMAALAGKPLPPEPAPNSNEAPREA
                     SQKRGKGYQQTSNDFFQGLFGN"
     gene            complement(464..>1978)
                     /locus_tag="VFR00_01675"
     CDS             complement(464..>1978)
                     /locus_tag="VFR00_01675"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007607278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HEU0157986.1"
                     /translation="PKVRLSRRGLSAAAGVGMLALASAAAALAPGQHALGLMSSSSAL
                     AAETTAPPAAAMPSFADLVERLKPTVVSVYVDAEIGNVAELGRGRQNQEFQGQNPFGQ
                     GSPFEFFFRQFGNGERMPAQRVRAQGSGFFISADGYVLTNNHVVEHAKSVQIRTTEGK
                     TLDAKVIGSDAKTDLAVLKVTSAGNFPFVKFAAQTPRVGDWVLAMGNPFGLGGTVTAG
                     IVSASGRDIGSGPYDDYLQIDAPVNKGNSGGPTFNTQGEVIGVNTAIYSPSGGSIGIA
                     FDIPADTAKTVAEQLKTKGSVSRGWIGVMVQPVTADIADSLGLQEPKGALIDDVQQGG
                     PAAKAGLQSGDVVLAVGNEEIKDARELARRIASVAPGQQLRLSVKREAGEQAITVTTA
                     AFPDEAKVTRQDRREPSSNPADAKLGLYLAPASQVEGAGSQGVVVVNVDPSGVAADKG
                     LQQGDVILDAGGKAVSTPNEVSTAVEAARKAGKHTILMRVRSAQATRFVALPLA"
BASE COUNT          313 a          658 c          682 g          325 t
ORIGIN      
        1 tacagccacg gctgcatgcg cgtgcaaaac ccgggtaaac tcgcggaaat tctgctgggc
       61 gaagacaagg gttggtcgcc cgagcaggtg cgcgatctga tggcgcgggg aggcaacaac
      121 gagatcacgc tgtcaacgca gatccccgtg cacgtgacct acttcaccgc cgtggcgggc
      181 gatgacaccc atgtcaagtt ccacgccgac ctctatgggc atgaccggcg cctgatggcg
      241 gcgctggccg gcaagccact gccccccgag ccggccccca acagcaacga ggcgccgcgc
      301 gaggcctccc agaagcgggg caagggctac cagcaaacca gcaacgactt cttccagggg
      361 ctgttcggca actgatcggg ccggtggcca ttccgggctt gcgcaaaggg cgccacggcc
      421 gagccgtggc gccctcttcg ttggcgaacc tcagcgcgcc cgctcaggcg agcggcaggg
      481 ccacgaagcg cgtggcctgc gcgctgcgca cgcgcatgag gatggtgtgc ttgccggcct
      541 tgcgcgccgc ctcgacggcg gttgagacct cgttgggcgt ggatacggcc ttgccacccg
      601 catccaggat cacgtcgccc tgctgcaggc ccttgtcggc ggcaaccccg ctcgggtcga
      661 cattgacgac gaccacgccc tgcgagccgg ccccctcgac ctggctggcc ggggccaggt
      721 acaagccgag cttggcgtcg gcggggttgc tgctgggctc gcgccgatcc tgacgcgtca
      781 cctttgcctc gtcgggaaag gcggccgtcg tcaccgtgat ggcttgttcg ccagcctcgc
      841 gcttgacgct caagcgcagc tgctggccgg gcgcaacgct ggcaatccgg cgcgccagct
      901 cgcgtgcgtc cttgatctcc tcgttgccga cggccagcac gacgtcgccg ctctgcaggc
      961 cggccttggc ggcgggaccg ccctgttgca cgtcatcgat cagggcgccc ttcggctcct
     1021 gcaggccgag gctgtcggca atatcggccg tgaccggttg caccatcaca ccgatccagc
     1081 cgcgtgacac ggaacccttg gtcttgagct gttcggcgac cgtcttggcg gtatcggcgg
     1141 ggatatcaaa ggcaatgccg atggagccgc ccgagggcga atagatcgcg gtgttgacac
     1201 cgatcacttc gccttgggtg ttgaaggtcg gcccgccgga gttgcccttg ttcacggggg
     1261 catcgatctg caggtagtcg tcgtagggtc ccgagccgat gtcgcgacca ctggcggaga
     1321 cgataccggc cgtcaccgtg ccgccaagac cgaagggatt gcccatcgcc aacacccaat
     1381 cgccgacgcg cggcgtctgg gcggcgaact tgacgaaggg gaaattgccc gcggaggtca
     1441 ccttgagcac cgcaaggtcc gttttggcgt cgctgccgat caccttggcg tcgagggttt
     1501 tgccctccgt ggttctgatc tgcacgctct tggcgtgctc gaccacgtgg ttgttggtga
     1561 ggacgtagcc gtcggcggag ataaagaagc cggagccctg ggcgcgtacc ctttgggcgg
     1621 gcatccgctc gccgttgccg aactggcgga agaagaactc gaacggcgag ccttggccaa
     1681 acgggttctg gccctggaac tcctggtttt ggcgtccgcg tccgagctcg gcgacattgc
     1741 cgatttcggc gtccacataa acggaaacga ccgtgggctt gagccgctcc accaggtcag
     1801 caaaactggg catggcggcc gccggcgggg cggtggtctc ggcggcgagc gcgctgctgc
     1861 tggacatcag gccaagagcg tgttggcccg gcgcgagcgc ggcagcggcg ctcgccagcg
     1921 cgagcatgcc aacgcccgcc gcggcggata ggccgcgccg ggagaggcgc actttcgg
//
LOCUS       DASTMT010000081         2590 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_2342911, whole genome shotgun sequence.
ACCESSION   DASTMT010000081 DASTMT010000000
VERSION     DASTMT010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2590
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2342911"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            204..797
                     /locus_tag="VFR00_01680"
     CDS             204..797
                     /locus_tag="VFR00_01680"
                     /inference="COORDINATES: protein motif:HMM:NF022439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2796 domain-containing protein"
                     /protein_id="HEU0157987.1"
                     /translation="MRIIRLAVLGSGLGLSLAAGVGNAQQRQLGAHEHGRGTLNLAIE
                     GTRVSLELEVPAADIIGFEHVAKTPRQKAAVAQAKKQLLAPQALFKFPGAAGCVVADA
                     SVDLEADGEHEHEQAKAGGDGAKTADAGQEANHSNFHAQYSFTCKTTASITVVEFGYF
                     QAFAGAQKLEVNVITAKGQSSFEVTRTKPRIDLASMM"
     gene            822..1553
                     /locus_tag="VFR00_01685"
     CDS             822..1553
                     /locus_tag="VFR00_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0157988.1"
                     /translation="MSVDSPAPRSPVIAVSDLRFTWPGRHAFALGIDRFVVPARQRLL
                     LIGPSGSGKSTFLGLLCGILTPLAGRIEILGRDIAPMSAAARDAYRAEHFGIIFQMFN
                     LLPYGTVIDNVLLPLSFAPERRRRVAAKGTAEQEAARLLGRLGLTAELVRGPSAARLS
                     VGQQQRVAAARALIGAPELIVADEPTSALDRDRQVAFLELLFSETSEAGATLIMVSHD
                     ESLAARFDKVMRLDEITIGGQKGSS"
     gene            1550..>2590
                     /locus_tag="VFR00_01690"
     CDS             1550..>2590
                     /locus_tag="VFR00_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0157989.1"
                     /translation="MIVLRLALQSLRNRWLTALLTVLAIAVSVVLLLGVEKIRTGARQ
                     SFADTISGTDLIVGARSGSLNLLLYSVFRIGNATNNVSWQSYQDISARPEVAWTVPLS
                     LGDSHHGFRVLGTTADYFRHYRFRQTHGLTFREGAAFNDLFDAVIGADVAAALGYRVG
                     DPIVVSHGLGSVAFLEHDDKPFRVAGILEKTGTPVDRTVHVSLEAIEAIHVDWQSGSR
                     LPGQSVSADEVRRMDLKPKAITAALVGLKSKLATFRLQRDINEYRAEPLSAILPGAAL
                     QELWGLVGIAETALTVVSAMVVATALLGMVTMILTTLNERRREMAILRSVGARPRTIL
                     ALLMAEAGVLTLT"
BASE COUNT          424 a          827 c          847 g          492 t
ORIGIN      
        1 tacgggggcc ccagggttaa gggggcacgc gcctaggggt gccctgccgc gctgccggtc
       61 acgttcccgc gaggcccctg tccgcgcgcc gattgctacg ttataacatc ccaacacctc
      121 cggggtgggc gcgcgcctcc cggtccgagc agcgccgctg cgaggccggt tttgcacggc
      181 gccagttggg aaggccacgc ctcatgcgca tcatccgtct tgccgtcctt ggttctggcc
      241 ttggccttag cctcgccgcc ggcgttggca atgcgcagca acggcagttg ggtgcccacg
      301 agcacggtcg cggtacgctc aatctggcca tcgagggcac ccgcgtcagc ctcgagcttg
      361 aggtgccggc tgccgacatc attggcttcg agcacgtcgc caagacgccg cgccaaaagg
      421 ccgccgtggc acaggccaaa aaacagcttc ttgcgccgca ggccctgttc aagtttcctg
      481 gcgcggcggg atgcgtggtg gccgatgcca gtgtcgatct cgaagccgat ggtgagcatg
      541 agcatgagca ggccaaggcc ggcggggacg gcgcaaaaac ggctgatgcc ggccaagagg
      601 ccaaccattc caattttcat gcgcagtatt ctttcacgtg caaaaccacg gccagcatca
      661 ccgtcgtcga atttggctac ttccaggcct ttgccggcgc ccagaagctc gaggtcaatg
      721 tgatcaccgc gaaggggcag agctcgttcg aagtgacgcg gaccaagccg cgcatcgatc
      781 ttgccagcat gatgtaggct gcgggtagcg gcatggtcgc catgagcgtg gactcgcctg
      841 ccccccggtc gcctgtcatt gccgtgtccg acctgcgctt tacttggccc ggtcggcacg
      901 cctttgcgct gggcatcgac cggttcgtcg tgccggcgcg ccagcggctg ttgctgatcg
      961 ggccgtcggg aagcggcaaa tcgaccttcc tcggcctcct ttgcggcatc ctgacaccgt
     1021 tggcgggacg catcgaaatc ctcggcaggg acatcgcccc catgtcggcg gcggcgcgtg
     1081 atgcctatcg cgccgaacac ttcggcatca tttttcaaat gttcaatctg ttgccctacg
     1141 ggaccgtgat cgacaatgtc ctgctgccct tgagttttgc gcccgagcgt cgcaggcgcg
     1201 tggccgccaa gggaacggcg gaacaggaag ccgcgcgcct gttggggcgc ctcggcctga
     1261 cggctgagct cgtgcgcggg ccctcggcgg ccaggctcag tgtcggacag cagcaaaggg
     1321 ttgcagccgc ccgtgcgctg attggcgcgc cggagttgat cgtggccgac gagccgacgt
     1381 cggctttgga tcgagatcgc caggtggcct ttctcgagct cctattcagc gaaaccagcg
     1441 aggcgggtgc gacgctcatt atggtcagcc acgacgaaag cctcgctgcg cgtttcgata
     1501 aggtcatgcg cctcgacgag atcacgattg gagggcagaa aggcagttca tgatcgtctt
     1561 gcgactggcg ctgcagtctt tgcgcaatcg ctggctcacg gcgctgctca ccgtgctggc
     1621 catcgcggtc agcgtcgtct tgctgctcgg cgtcgaaaaa attcggaccg gcgcgcgcca
     1681 gagcttcgcc gacaccattt ccggcaccga tttaatcgtc ggggcgcgca gcggcagcct
     1741 caacctcttg ctgtattcgg tgtttcgcat cggcaacgcc accaacaatg tgtcttggca
     1801 aagctatcag gatatctcgg cgcgacccga ggtggcctgg accgtgccgc tgtcattggg
     1861 cgacagtcac cacggctttc gcgtgctggg cacgacggcc gactatttca ggcactaccg
     1921 atttcggcaa acgcacgggc tcacgtttcg tgagggggca gccttcaacg acctgttcga
     1981 tgccgtcatc ggggccgacg tcgccgccgc cctgggatat agggttggcg acccgatcgt
     2041 ggtctcgcat ggactgggtt cggtcgcttt tctagagcac gacgacaaac cttttcgcgt
     2101 cgccggtatt ctggaaaaga cgggcacgcc ggtcgatcgc accgtgcatg tcagcctgga
     2161 ggcgatcgaa gccattcacg tggattggca aagcggcagc cgtcttcccg gccaaagcgt
     2221 atcggccgac gaggtgcgcc ggatggactt gaagccgaag gcgatcacgg cggccctcgt
     2281 tggtctcaaa tcgaagttgg ccacctttcg tctgcagcgg gacatcaacg agtatcgggc
     2341 cgagccgctc tcggccatcc tgccgggggc cgctctgcag gagctctggg gcctggtcgg
     2401 catcgccgag acggctttga cggtggtgtc cgccatggtg gtggcgaccg ccctgctcgg
     2461 catggtcacc atgatcctga ccaccctgaa cgaacgccga cgggagatgg cgatcctgcg
     2521 ctcggtgggc gcccggccgc gcaccattct ggcgttgctg atggcggagg ccggcgtgct
     2581 cacgctgacg
//
LOCUS       DASTMT010000082         2336 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_3327374, whole genome shotgun sequence.
ACCESSION   DASTMT010000082 DASTMT010000000
VERSION     DASTMT010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2336)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2336)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2336
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3327374"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..683)
                     /locus_tag="VFR00_01695"
     CDS             complement(<1..683)
                     /locus_tag="VFR00_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019404523.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEU0157990.1"
                     /translation="MTAWERFLETFFKPDMIARYWPSIAQGVLVTVEIAAAVVVTGLA
                     LGLLLAALRAYRLALCNALIVIFVDVFRSLPPLVVILLVYFGLPSVGVNLPSFVVLWG
                     VLSLVLAAFAEEIFWAGIISTPQGQWEAARSTGLGFSETLAFVVMPQAIRLGVPPLTN
                     RTIAITKNTALGTVIGVGEILNQATAAQSFSGNATPLMLGAIAYIGLFVPVVAAGRWI
                     ETRFAWRRV"
     gene            complement(688..1419)
                     /locus_tag="VFR00_01700"
     CDS             complement(688..1419)
                     /locus_tag="VFR00_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006201493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEU0157991.1"
                     /translation="MAILTVSGLRKSFGASEVLKGIDLSVGERELVFIIGPSGSGKST
                     LLRCLTRLEEPSAGTVSFAGVEVTAPATNLKLVRRSMGMVFQSFNLYPHMTALGNVTL
                     ALRRVLKKTRAEADALGRSALAGVGLADKALSYPSELSGGQQQRVAIARALALDPKIV
                     LFDEPTSALDPELVGSVLSVMRDLRARGMTMLVVSHELRFARDAADRVVFMDAGRIVE
                     EGTPEKIFNSPRETRTKAFVAQLTR"
     gene            complement(1471..2307)
                     /locus_tag="VFR00_01705"
     CDS             complement(1471..2307)
                     /locus_tag="VFR00_01705"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEU0157992.1"
                     /translation="MRLGIVLVSTLCVLALPGIGAAQQPLRTAVDGTFAPHAMPRLEG
                     GIQGFNVDLANEVGKRLKRPVEIQAMQFSGILPALQAGSIDFVAAPVTVTKERAENYI
                     FTEGYLNTDYQFLTKKGEPPVTDLKQLKGKVIAVNKGAIYDSWARALEAEIGWSVESF
                     GTQTDAVQAVLAGRAYANVAGNTAIQWAVKQQPQLQLSYLYATGLVWAMPVRKDQLAL
                     RAELENAIECMKKDGTMATMHERWFGTKPAANSPAVTIYPGTGVPGTPGYEAAPREPK
                     CD"
BASE COUNT          437 a          792 c          730 g          377 t
ORIGIN      
        1 tacaccctcc gccaggcaaa ccgcgtctcg atccagcgac cggcggcaac caccggcacg
       61 aacaagccga tgtaggcgat cgctcccagc atcagcggcg tggcgttgcc tgagaaggac
      121 tgcgccgccg tggcctggtt caaaatctcg ccgacgccga tcacggtgcc aagcgcggta
      181 ttcttggtaa tggcaatggt gcgattggtc aggggcggca cgccgaggcg gatcgcctgc
      241 ggcatcacca cgaaggcgag cgtctcggaa aagccaaggc cagtggaacg cgcggcctcc
      301 cactggccct gtggggtcga gatgatgccc gcccaaaaaa tctcctcggc gaaggcggca
      361 agcaccagcg acagcacccc ccacagcacg acaaagctcg gcagattgac gcccacgctg
      421 ggcaggccaa agtagacgag caggatcacc accagcggcg gcagcgagcg gaagacgtcg
      481 acaaaaatca cgatcagcgc gttgcacagc gccagacgat aggcgcgcag cgcggcgagc
      541 agcagcccca aagccaagcc cgtgaccacc acggccgcgg cgatttcgac cgtcaccagc
      601 acgccctggg cgatgctcgg ccaatagcgg gcgatcatat ccggcttgaa aaaggtttcg
      661 aggaagcgct cccaggccgt catgccatca ccgggtcaac tgggcgacga acgccttagt
      721 gcgtgtctca cgcggactat tgaagatttt ctcgggcgtc ccctcctcga cgatgcggcc
      781 tgcgtccatg aagacgacgc ggtcggcggc gtcgcgggca aagcgcaact cgtggctgac
      841 gaccagcatg gtcatgccgc gtgcccttag atcacgcatg acgctcaaga ccgagccgac
      901 caactccgga tcgagcgccg aggtcggttc gtcgaacagc acgattttgg ggtccagtgc
      961 cagcgcccgg gcgatcgcca cgcgctgctg ctggccaccc gagagctcgc tcggatagct
     1021 cagcgccttg tccgccaggc caacgccggc cagcgcgctg cggcccagag cgtcggcctc
     1081 cgcgcgcgtc tttttgagca cgcggcgcaa tgccagcgtc acgttgccga gcgcggtcat
     1141 gtgcggataa agattgaagg attggaacac catgcccatg gagcggcgca ccagtttgag
     1201 gttcgtcgcc ggcgccgtca cctcgacccc cgcaaagctc accgtgccgg cgctgggttc
     1261 ctcgagccgg gtcagacagc gcaatagcgt gctcttgccg gagccggacg ggccgatgat
     1321 gaacaccaat tcgcgctcgc cgacgctgag gtcgataccc ttgaggacct cgctcgcccc
     1381 gaagctcttc ctgaggcccg agaccgtcag tatcgccatg ccggcaggat cactcttgca
     1441 aagggaaaag cgcggcgccg cggcgccccg ctagtcgcac ttcggttcgc gcggcgctgc
     1501 ctcataaccc ggcgtgccgg gaacgccggt gccgggatag atggtcaccg ccggcgagtt
     1561 cgcagcaggc ttggtgccaa accagcgctc gtgcatggtg gccatggtgc catccttttt
     1621 catgcattcg atggcgttct cgagctccgc gcgcagcgcc agctggtcct tgcgcaccgg
     1681 catcgcccag accagtccgg tcgcatacaa ataggacagt tgtagctgcg gctgctgctt
     1741 gacggcccat tggatcgccg tgttgccggc aacgttggcg taggcccggc ccgccagcac
     1801 ggcctgcacg gcatccgtct gggtgccgaa actctcaacg ctccagccga tctccgcctc
     1861 cagtgcgcgc gcccagctgt cgtagatcgc ccccttgttg acggcgatca ccttgccttt
     1921 caactgcttc aaatcggtga ccggcggctc acccttcttg gtgaggaact gatagtcggt
     1981 gttgagatag ccctcggtga aaatgtagtt ttccgcgcgc tccttggtca ccgttaccgg
     2041 cgcggcgaca aagtcgatgc tgccggcctg caaggccggc agaatacccg agaactgcat
     2101 ggcctggatc tcgaccgggc gcttcaggcg cttgcccacc tcgttggcaa gatcgacgtt
     2161 gaaaccctga ataccgccct cgagccgcgg catggcgtgt ggcgcaaagg tgccgtcgac
     2221 ggcggtcctg agcggctgct gcgccgcccc gatgccgggc agcgccagca cgcagagcgt
     2281 cgagaccaga acaatcccca ggcgcattga tcgtcctcca tcgcctcaag cgtcgc
//
LOCUS       DASTMT010000083         3007 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_4946888, whole genome shotgun sequence.
ACCESSION   DASTMT010000083 DASTMT010000000
VERSION     DASTMT010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3007
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4946888"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(217..513)
                     /locus_tag="VFR00_01710"
     CDS             complement(217..513)
                     /locus_tag="VFR00_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009493165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyU family transcriptional regulator"
                     /protein_id="HEU0157993.1"
                     /translation="MLSSLKAIWARLASGPSGGAAATAVEPVDYKGFRIRPAPYLSGS
                     QYQTAGSIEKDTPDGRKEHKFIRADTYASREDAIAFTVVKAKQMIDLQGERLFG"
     gene            complement(696..3005)
                     /locus_tag="VFR00_01715"
     CDS             complement(696..3005)
                     /locus_tag="VFR00_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012045863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEU0157994.1"
                     /translation="MTELSTHSKISISNVSRRNLLKGVAATGGLVLAAQLVGARGALA
                     GYPTGASAMPNGVISDPKVFVSIGNDGVVSIIAARAEMGTGAARTALPMMVADEMGAD
                     WARVRVVQSPGDEKTYGNQDTDGSRSVRHFIQPMRQCGAAARQMLEAAAAKKWGVDVG
                     EVETQVHEVVHKASGRKLGFGELAADAAQLPVPAEDKIKLKDPSAFRYIGKGNVRPTD
                     QVDITTGHAVYGQDITLPGMKFAVIARPPVVGGKVASLDSSAALKVPGVEKIVTLQPT
                     PAPYKFNPLGGVAVIGKSTWAVLKGREALKITWEAGPNKVYDSKAYRAQLEESVKKPG
                     KVERNVGDVDKALASAAKVITAEYYAPHIHHATMEPPAAAARMTDGKWEVWAPVQSPG
                     GARDDIAKALGIKPEEMVVHPTLLGGGFGRKSKCDFAIEAALLSKEMGGTPVKVVWTR
                     EDDLQHGFYHTVTAERFEAGVDANNKVIAWRHRSAAPSILSTFAPDPKHPFDIELGMG
                     WVDTPFNVPNIRMESGEAQSHVRIGWFRSVNNVVHAWSTQSFVAEIAQQLGKDPKDFL
                     LELIGPDRTIDPREQLTTKWWNYGEPFETYPIQTGRLRRVAELAAKEAGWGKQLPKGQ
                     GLGIAAHRSFVSYIATVVHVEVGEKGKITIPRVDTAIDCGYCVHPERVRSQIEGAAVM
                     GLTLAKYSEVTAKNGAIEQTNFNDYPLVRIDESPKETRVHIVENGLDVPPSGVGEPGV
                     PPFAPALFNAIFAATGKRLRQLPLGEQLA"
BASE COUNT          463 a          965 c          992 g          587 t
ORIGIN      
        1 aacgatcgtc tccccggtga gcgcgcccgg agagagcttc cctgtttaga tatgcgcgct
       61 ggaggtctcg caaaggtgcg cgacgattac agtttggaca gagtggatta caaatttgac
      121 acgcggctcg gtggaaccgc agaccggtgc gctgatgcgc cgctggcgcg ccgcgctcag
      181 gcgcggggcc aagcgtttgc tggctttggc agaggttcag ccgaacaggc gctcgccctg
      241 cagatcgatc atctgcttgg ccttcactac ggtgaaggcg atggcatcct cgcgactggc
      301 ataggtatcg gcgcggatga atttatgttc cttgcggccg tcgggggtgt ccttctcgat
      361 gctgccagcg gtctggtact ggctgccgga gagatagggc gccggacgga tgcgaaagcc
      421 cttgtaatcg acgggctcga cggcggtggc cgcggcgccg ccgcttggcc cgctcgcaag
      481 gcgggcccag atcgctttca ggctcgagag catgtcacat ccgctccaac tggcgcaatt
      541 gtcgcgtagc gctggcggcc tgtccagcca ccagcgctgc gcgttcctgg caaaaaatcg
      601 gccccaacgg gggttggggc cgatgcgctt tgcgttcgcg aaccgagtgg gggtgccaag
      661 cgcctgcttg gcacccggcc ccccttgggt tagtctcagg ccagttgctc gccaagcggc
      721 agctggcgca gccgcttgcc ggtggcggca aagatggcgt taaacaaggc tggcgcgaag
      781 gggggaaccc ctggctcgcc aaccccgctc ggcggcacat cgaggccatt ctcgacgatg
      841 tgcacgcgcg tctccttggg cgattcatcg atgcgcacga gcggatagtc gttgaagttg
      901 gtctgctcga tggcgccgtt cttggcggtg acctcgctgt atttggcgag ggtcaggccc
      961 atcacggcag cgccttcgat ctgcgagcgc acccgctcgg gatgcacgca gtagccgcag
     1021 tcgatggcgg tatcgacgcg aggaatggtg atcttcccct tctcgccgac ttccacgtgc
     1081 accaccgtgg caatgtagct gacaaagctg cggtgcgcgg cgatgccgag cccttgccct
     1141 ttcggcagct gtttgcccca gcccgcttcc ttggcggcga gttcggccac ccttcgcaac
     1201 cgcccggttt gaatgggata ggtctcgaag ggttcaccgt agttccacca cttggtcgtg
     1261 agctgctcgc gcgggtcgat ggtgcgatcg ggcccgatca gttccaacag aaagtccttc
     1321 gggtccttgc ccagctgctg ggcgatctcg gccacgaagg actgcgtcga ccaggcatgg
     1381 acgacgttgt tgaccgagcg gaaccagccg atgcggacgt ggctctgcgc ctccccgctc
     1441 tccatgcgga tgttgggcac gttgaacggc gtgtcgaccc aacccatgcc gagctcgatg
     1501 tcgaagggat gcttcggatc gggcgcgaag gtggacagaa tcgacggtgc ggcgctgcga
     1561 tgccgccagg cgatcacctt gttgttggcg tcgacgccgg cttcgaagcg ctctgcggtc
     1621 acggtgtgat agaagccgtg ctgcaggtcg tcctcacgcg tccacaccac cttgaccggc
     1681 gtgccaccca tctccttgga gagcaacgcc gcctcgatgg caaagtcgca cttggatttg
     1741 cgcccgaacc cgccgccgag cagcgtgggg tgaaccacca tctcctcggg cttgatgcca
     1801 agcgccttgg cgatatcgtc acgcgccccg ccggggcttt gcaccggcgc ccacacttcc
     1861 catttgccat ccgtcatgcg cgccgcggcc gccggaggct ccatggtcgc gtgatgaatg
     1921 tggggagcat agtattcggc cgtgatgacc ttggccgcgg acgccagcgc cttgtcgacg
     1981 tcgccgacgt tgcgctcgac cttaccgggc tttttgacgc tctcctcgag ctgggcgcga
     2041 taggccttgg agtcgtagac cttgttgggt cccgcctccc aggtgatctt gagcgcctcg
     2101 cggcccttga gaaccgccca ggtgctcttg ccgatgacgg caacgccgcc caacgggttg
     2161 aacttgtagg gcgccggtgt cggctgcagg gtgacgatct tttccacacc cggcactttc
     2221 aaggctgcac tgctgtcgag cgaagccact ttgccgccga ccaccggggg tctggcgatg
     2281 acggcgaact tcatgcccgg cagcgtgatg tcctgtccgt acacggcgtg cccggtggtg
     2341 atgtcgacct ggtcggtcgg gcgaacgttg cccttgccga tatagcggaa ggcgctgggg
     2401 tccttgagtt tgatcttgtc ttccgccggc accggcagct gggcggcatc ggcggcgagc
     2461 tcgccgaagc ccagcttgcg acccgaggcc ttgtgcacga cctcgtgcac ctgcgtctcg
     2521 acctcgccga cgtcgacgcc ccacttcttg gcagcggccg cttccagcat ctgccgcgcc
     2581 gcggcgccac attggcgcat cggctgaatg aaatggcgca cgctgcgcga gccatccgtg
     2641 tcttgattgc cgtaggtttt ctcatcgccg ggtgactgca cgacgcggac gcgtgcccaa
     2701 tcggcgccca tctcatcggc gaccatcatg ggcagcgccg tacgtgcggc cccggttccc
     2761 atttcggcgc gggcagcgat gatgctgacg acgccatcat tgccgatcga gacgaacacc
     2821 ttggggtcgg agatgacgcc attgggcatg gccgaggcgc cggtcggata gccggccagt
     2881 gcgcctctcg cgcccacgag ctgggcggca agcacgagcc cgccggtggc cgccacaccc
     2941 ttcaacagat tgcggcggct gacattgctg atgctgattt tgctatgtgt gctgagctcg
     3001 gtcatgt
//
LOCUS       DASTMT010000084         3035 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_2999727, whole genome shotgun sequence.
ACCESSION   DASTMT010000084 DASTMT010000000
VERSION     DASTMT010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3035)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3035)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3035
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2999727"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            186..1718
                     /locus_tag="VFR00_01720"
     CDS             186..1718
                     /locus_tag="VFR00_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009798421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU0157995.1"
                     /translation="MGAAQGQPAGPDLTQGIASAELVDGGMLAGHVGEDAVLLARSGN
                     EIFAIDAKCSHYGGPLADGLLVGATVRCPWHHACFSLASGEALDAPAFSPLGCWQVEE
                     RDGRLFVRRKKASQPERESPPRVAAAPDRIVIVGGGAAGFAAAEMLRRLKYQGGISLL
                     SADDAAPVDRPNLSKDYLAGNAPEDWLPLRPDAYYAEQGIHLRLKAKVTALDLRAKAV
                     ELAGGDRLEYDRLLLATGAEPVRLTIAGANLPHVHVLRSLADCKSIIATAAAAKRAVV
                     LGASFIGLEVAASLRARGLEVHVVAPEARPMERVLGPQLGELVRALHLEHGVVFHLPD
                     VASAITGTHVELKSGARLAADLVVAGIGVKPRLELAEQAGLPIDRGVLVDAALETGAP
                     GVFAAGDIARWPDPRGGSLRVEHWVVAERQAQVAARNMLGLGQKFTAVPFFWSQHYDV
                     AINYVGHAERWDEIDVAGDPAARDCLVRYRLGGRTLAVASINRDVASLEAELAMEREL
                     LA"
     gene            2022..2747
                     /locus_tag="VFR00_01725"
     CDS             2022..2747
                     /locus_tag="VFR00_01725"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEU0157996.1"
                     /translation="MSQQVKAELAPTGVLRAGINLSNFLLVTGKTDAGDPVGVAPDMA
                     SEIARRLGVPVRFVTYKTPGELADMADAGAWDIGLIGAEPQRAEKISFTPAYVEIEAT
                     YLVPAGSALKSIADVDRPGVRIAVTGRSAYGLWLDRNIKQAQLVKSGTLDSAYEQFVA
                     DKLDALAGLRPRLLSDVEKLPGARILDGQFTAVQQAVGTARRNSAAADYLRSFVAEAK
                     ASGFVGRLIEKHKVRGLSVAPPA"
BASE COUNT          500 a          952 c         1082 g          501 t
ORIGIN      
        1 tttggggttg tgccgcattg gatgccgcaa cgcccaattg gttcccacct cggcctgtgc
       61 cttaatcgga cccttcggcg ggcttggaac ttttcatggg cttgtgcatc taagccattg
      121 ccaacgggcc gagcggcccc aggcaccgta agctcggggg agccagaagc gcacaggagg
      181 tcgtcatggg agctgcacaa ggtcagccgg cagggcccga cctgacgcaa ggcattgcca
      241 gcgccgagct ggtcgatggc ggcatgctgg ccggtcacgt cggcgaggat gcggttttgc
      301 tggcgcggtc cggcaacgag atctttgcca ttgacgccaa gtgctcccat tacggcggcc
      361 cgcttgccga tgggctgctg gtcggggcca ccgtgcgctg cccctggcat catgcctgct
      421 ttagtctcgc cagcggcgag gcgctcgatg caccggcgtt ttcgccgctc gggtgctggc
      481 aggtggaaga acgcgatggc cggctgttcg tgcggcgcaa gaaggcgagc cagcccgagc
      541 gcgaaagccc gccgcgggtg gccgcggccc ctgaccggat cgtcattgtc ggtggcgggg
      601 cggccggctt cgcggccgcc gagatgttgc gtcgtctcaa ataccaaggt ggtattagct
      661 tgctcagcgc cgatgacgct gcgccggtcg atcggcctaa cctgtcgaag gactatctgg
      721 ccgggaatgc gcccgaggat tggctgccat tgcgaccgga cgcctattat gccgaacaag
      781 gcattcacct gcgcctcaag gccaaggtca ctgccctcga cctgcgcgcc aaggcggtgg
      841 agctcgccgg cggcgatagg ctcgagtatg accggttgtt gttggccacg ggtgccgagc
      901 cggtgcggct aacgattgcg ggtgcgaacc tgccccacgt gcacgtcctg cgctcgctgg
      961 ccgactgcaa aagcatcatc gccacggccg cggcagccaa gcgcgcggtc gtcctcggtg
     1021 caagcttcat cgggctcgag gtggctgcat ccctgcgcgc ccgtggcctg gaggtgcacg
     1081 tggtggcgcc ggaggcgcgg ccgatggaac gcgtgctcgg gccgcaactc ggcgagttgg
     1141 tgcgggcgct gcatctcgag cacggcgtcg tcttccacct gccggatgtg gcgagcgcca
     1201 ttaccggcac gcacgtcgag ctaaaaagcg gggccaggct agcggcggat ctcgtcgtgg
     1261 ccggcatcgg ggtaaaaccg cgccttgaac tggccgagca ggccggcctc ccgatcgacc
     1321 gcggcgtgct tgtcgacgcc gccctggaaa cgggcgcccc tggcgtgttc gccgccggtg
     1381 acatcgcccg ttggccggat ccgcgcggcg gcagtctgcg cgtcgagcat tgggtggtgg
     1441 ccgaacggca ggcgcaggtc gccgcccgca acatgctggg cctagggcaa aaattcacag
     1501 ccgtgccgtt cttctggagc cagcattacg acgtggcgat caactatgtg ggtcacgccg
     1561 agcgctggga tgaaatcgac gttgctggcg acccggcggc gcgggactgt ctggtgcgct
     1621 atcgcttggg tggccgcacc ctggcggtcg cttctatcaa ccgcgatgtc gccagccttg
     1681 aagcggaact ggcgatggag cgcgagctcc tcgcctgaga cggtgaggcc aagcacgtcc
     1741 atctcgcgtt cggccatact gccgtggcgc tgtcgcgccg gggccggagc aagggcccga
     1801 acgacgcagc cacaacctca gccgaatggc cgcggaaaga cggctgacgg agccgctggc
     1861 gtttgccgac ctgcagcggc taaagtgtgg gctcgaccgt ggcgaggtag gccgtgacca
     1921 catggatagc cgtggctggc atcattgccg cttttttcgt tgggatattg ccgcaggcgg
     1981 cctcgcccca atcagcaccg gccaagcagg aggaagcgct catgtcgcaa caagtcaaag
     2041 ccgagttggc accgacgggc gtgctgcgcg cgggcatcaa cctgtcgaat tttctcctgg
     2101 taacgggcaa gacggatgcc ggtgaccccg tcggcgtggc gcccgacatg gccagcgaaa
     2161 tcgcccggcg tctgggcgtg ccggtgcgct ttgtcactta caagacaccc ggcgaactgg
     2221 cggacatggc cgacgccggt gcctgggata tcggcttgat cggtgccgag ccgcagcgcg
     2281 ccgaaaagat cagcttcacg ccggcctacg tggagatcga ggcaacctat ctggtgccgg
     2341 cgggctcggc cctgaagtcg atcgccgacg tcgaccgacc gggggtgcgc atcgccgtga
     2401 cgggccgcag cgcctatggc ctctggctcg atcgcaacat caaacaggcc caactggtaa
     2461 aatcgggtac gctcgacagc gcctatgagc agttcgtggc cgacaagctc gacgccttgg
     2521 ccggactgcg gccacggctg ttgtcggacg tggagaagct gcccggcgca cgcatcctcg
     2581 atggccagtt cacggccgtc cagcaggcgg tgggaacggc gcgccgcaat agcgcggcag
     2641 ccgactatct gcgcagcttc gtggcagaag caaaggcttc gggttttgtc ggtcgattga
     2701 tcgagaagca caaggtgcgt ggtctctccg tggcgcctcc cgcctaagcg ccctgttgag
     2761 gggtaggcct gcagccccgg cggcggtgtc tgtggaacgc cgagggccgg acctcgtagg
     2821 ggatgcaggg gccggaggct ccggcggcga actcaatcgg cccttggcaa ggggaagcgg
     2881 gggcaaggaa accgggggca aggaaagcga gcaaggaaag cgagggcaag ggaagcgggg
     2941 gcgggcaacg ccgccatgcg ggtacttgcc ccaagttcaa tccgcggccg cgcgcggcac
     3001 ttggctgcga ggcatagggt cgtgcccaaa ggctt
//
LOCUS       DASTMT010000085        10614 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_376485, whole genome shotgun sequence.
ACCESSION   DASTMT010000085 DASTMT010000000
VERSION     DASTMT010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 10614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10614
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_376485"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..547
                     /locus_tag="VFR00_01730"
     CDS             <1..547
                     /locus_tag="VFR00_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011083558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEU0157997.1"
                     /translation="RFTSGECAIFLTSSGFFGNVRANAKFEFGNAMMPYYPDVQGAPQ
                     NSIIGGASLWVMGGKSAAEYKGVAKFFTFLSDTDRQVELHTKSGYLPITRAAYDKTKA
                     SGFYKEFPYLETPLLELTNKEPTENSRGLRLGNMVQLRDVWAEEIEAALAGKKSAKAA
                     LDEAVARSNTMLRQFERTATR"
     gene            720..1805
                     /locus_tag="VFR00_01735"
     CDS             720..1805
                     /locus_tag="VFR00_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009763235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-TA family PLP-dependent enzyme"
                     /protein_id="HEU0157998.1"
                     /translation="MPIEQLKAAIANDFGTPALVVDLDRVERNIARVQALCDRAGMAN
                     RPHIKTHKSPALAKLQLAAGAKGITCQKLGEAEVMADAGIDDILISYNLLGEARIGRL
                     AALSKRVTVTVAADNPTVIAALAGAGKIAGRPLGVLIECDTGRKRAGVEEPREAIALA
                     RLIKQHPDLSFQGFLFYPTEQSWPQTQRFYDAALSGVREAGLEPRMVSTGGTPNLVNL
                     GRLAGATEHRAGTYIFNDRMMLACGAATQEDCALSVFATVVSRAAADRGILDAGSKTL
                     TADTGGLEDGFGLILEYPQATIRAFAEEHGFLDLSRSNDRPSVGDVVRVVPNHVCVVV
                     NMFDRMIAVRGDRIVEVLPVAARGKLS"
     gene            1994..3355
                     /locus_tag="VFR00_01740"
     CDS             1994..3355
                     /locus_tag="VFR00_01740"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HEU0157999.1"
                     /translation="MSEALTRSLGNFVSGLRLEAIPAPALSVVHTGFADCVATLIAGS
                     IEAPPRILHKALAPPAGEASLYLVGPRVPAPDAAWINGTAAHALDYDDVSLRGHPSTV
                     LVPAILAEAEAIGASGAEMATAYVAGYEVWAELAGRDPDQHHQKGWHPTGIFGAIAAA
                     AACASLRGLDADQTTQALALGASHSGGLVANFGTMTKPYHAGKAAHAGVISARLAAAG
                     FTASPDALEHPLGFLAAVSPSGAADRERKIAAGRDWQILSQGLSVKKYPLCYCTHRAI
                     DGMLDLLREQEVGAADVKSITVSTSRRNATILRNARPQTGLEAKFSMQFAMASSIVAG
                     QVGLAELTDGFVQRQDVQALLDKVRIEPDDRPDPKRSGAAPYDLVVIETAGGRRLESA
                     RISDERGSPQLPLSSDELWAKFESCFRVGNAALPARVIFDALMSLERQPGVAAFSGLA
                     QAA"
     gene            complement(3357..3557)
                     /locus_tag="VFR00_01745"
     CDS             complement(3357..3557)
                     /locus_tag="VFR00_01745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0158000.1"
                     /translation="MAKRDAEKELLAGPHNGPESAPRVPGRRRSGYQRQSVGEAIAKS
                     FIRSVASSLGRILARAITGRMR"
     gene            complement(3580..5052)
                     /locus_tag="VFR00_01750"
     CDS             complement(3580..5052)
                     /locus_tag="VFR00_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like domain-containing protein"
                     /protein_id="HEU0158001.1"
                     /translation="MVESQILEQEGKIFLGQSVVPEYLELKLANRHGLVTGATGTGTT
                     VTLQVLAEGFSNAGVPVFAADIKGDLSGISAMGEKKEGLSKRAQEIGLKDWTTAAFPT
                     VFWDLFAEQGHPIRATVQDMGPLLLARLMELNETQEGVLNILFRVAADEKLPLLDLKD
                     LRSMANDVGQRGKSLMTKYGNVSAVSVGAIQRRLLVLEEQGANNFFGEPALEIKDFMR
                     TAPDGRGTVNILAAERLMKTPRLYATFLLWLLAQLFDKLPEVGDPDKPKLVFFFDEAH
                     LLFSEAPKALLEQVERVTRLIRSKGVGVYYVTQNPRDVPNSVSAQLGNRVQHALRAFT
                     PIEQKGIRAAAETFRPNPKLNLERVIQELKVGEALVSLLHGGGEPSMVQRTLIRPPSG
                     RLGPITPEERKSKIEASPIYGKYEEAVDRDSAYEILQKRTATAAAQQQADANSWGNIL
                     MGGGTGRRQGYGETFAKSMMRSVASSIGNAIVRAMLGGRR"
     gene            5458..6012
                     /locus_tag="VFR00_01755"
     CDS             5458..6012
                     /locus_tag="VFR00_01755"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEU0158002.1"
                     /translation="MDLSTESLFQVLESRGGGRYGLSAVTQKEHALQAAALAADRGLG
                     DQLVIAALFHDVGHLLVGEDVDLAEAGIDDLHEETSAKALEALFGKDVAEPVRLHVAA
                     KRYLCAVNPAYYDKLSEDSRRSLALQGGPMSPGEVAAFDGLAHRAAALALRIIDDEAK
                     VEGLKTPPLESYRALAARLQGGAA"
     gene            6318..6479
                     /locus_tag="VFR00_01760"
     CDS             6318..6479
                     /locus_tag="VFR00_01760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0158003.1"
                     /translation="MDAERPATQELCDAILNALGRDERLRRDYVRRRWRGLIVEARAI
                     RAAAAPGEG"
     gene            complement(6579..6983)
                     /locus_tag="VFR00_01765"
     CDS             complement(6579..6983)
                     /locus_tag="VFR00_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018456915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HEU0158004.1"
                     /translation="MAITGGCHCGAIRYQAEGEALNHALCHCTDCRRHAGAPMVGWTM
                     YPRERVTVTKGSPKVYASSQDGRRYFCADCGTGLFFSNDVVLPGLLDIQSATYDDPDA
                     VPAQAHIQVADRIGWMQRAHELPEFERYPPRA"
     gene            7333..9204
                     /locus_tag="VFR00_01770"
     CDS             7333..9204
                     /locus_tag="VFR00_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HEU0158005.1"
                     /translation="MTDDLEPISTEFEAASREAWLKLVDKVLKGGSFERQLVSETADG
                     LRIDPLFTRSEAAPVNSVQVGRPAAAGHRWDIRQRHGEPDPKLCNGAILEDLEGGVNS
                     LLLQIEAPGQWGLGYGAEPLARALRGVRLEQVPIALDARENTLDAAGSLIEIWREAGV
                     GERQRRGAFNYDPLGVLAKTGTLYYAPDRACEIAAKFASDQQAMPNVTALLADARPYH
                     EAGAGEAQELAALLATLVAYLRACEAGGLPPRAAFGKIGFAVAIDADLFVTIAKLRAA
                     RSLLARVAQACGVPEAAEALTLNASTSERMLAKRDPWVNMLRATVACAGAALGGADSI
                     SVLPFTWALGKPDRFALRIARNTHLVLQEESALGRVADPAHGAWFMEQLTTELAEKAW
                     ALFQAIEHAGGMARALQSGFLAAEVSKVARARSGRIAQARLELTGVSAFPRLGDDGVS
                     VAPHPMAPPVVRGGTSAEPFPLRRLAEPFERLRDAADRHLARTGQRPQVFLASLGEAA
                     VHSGRTTWMQNFLAAGGIEAVPSPPLHNSAAAGKAFAESGTCAVSICSSDAVYAELAE
                     ATAGVLKAAGAKQVLLAGRPKGQEEALRAAGVDEFVFAGGDALATLNRLHEVLDVTS"
     gene            9191..9709
                     /locus_tag="VFR00_01775"
     CDS             9191..9709
                     /locus_tag="VFR00_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011085839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HEU0158006.1"
                     /translation="MSHPEPSQTPRHTGGCQCGAVRFAVYADPLKIGICHCRMCQKAV
                     AGPFAVLAEVPGAQFAWTRGRPATFQSSTRAVRDFCPACGTPLSYRHPGGPIIELLTG
                     AFDQPARLPPTYAVGTESMLAWLADIAKLPGKTTANNLASQPTADIVGFQHPDHDTDR
                     TWQPPRRASPDA"
     gene            9829..>10614
                     /locus_tag="VFR00_01780"
     CDS             9829..>10614
                     /locus_tag="VFR00_01780"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEU0158007.1"
                     /translation="MRTLFDFAAKRAELSPDALAFAEFEPESKLCFGEFNARAERGAS
                     LLERQFGSGDRIAVLCHNCTGFFELLFACGKIGVILVPLNWRQTPTELTPILLDCGAN
                     MLLHDGPTAPLAEALAASTGVRLCTFAAYAQMRAETSPNPRRETAWPSDRIWYLLYTS
                     GTTGRPKAVIQTVGMALANAINVQQATALSSLDRTVNFLPLFHTAGINLHTLPVFIAG
                     GSSTVLAKFEVDPLFDLIAAGRVSVFFGVPSIYQALSLSSRFAG"
BASE COUNT         1739 a         3618 c         3466 g         1791 t
ORIGIN      
        1 acgtttcacc tcgggcgagt gcgcgatctt tctcacctcg tccgggttct tcggcaatgt
       61 tcgcgccaac gcgaagttcg agttcggcaa tgcaatgatg ccgtactatc ccgatgtcca
      121 gggtgcaccg cagaactcca tcatcggcgg cgcctcgttg tgggtgatgg gcggcaagag
      181 cgcggccgag tacaagggcg ttgccaaatt cttcaccttc ctgtccgaca ccgaccgcca
      241 ggtggagctg cacaccaaat cgggttatct gcccatcacc cgggccgcct acgacaagac
      301 caaggcatcg ggcttctata aggagtttcc ctacctggaa acgccgttgc tcgagctgac
      361 caacaaagag ccgacggaga actcccgcgg tctgcggctc ggcaacatgg tgcaattgcg
      421 cgatgtatgg gcggaggaga tcgaggcggc gctggccggg aaaaaatcgg ccaaagcagc
      481 gctggacgag gccgtcgcgc gcagcaacac catgctgcgt caattcgaac gcaccgcgac
      541 gcgctaacaa agcctggcgg gggcgggcaa ttccccgccc ccgcgtttgg ccggcaccgt
      601 tgctcgcgcg tcctccgcgc cctgggggtg gcgtcttagg gcgtcgcggc ccgcgcgcgg
      661 gtggatcgca cgcccggcct gtgagaggag gtctcggtgc agcacgggag ggctaggcca
      721 tgccgattga gcaactgaaa gccgcgatcg ccaacgattt tggcacgcct gccctcgtcg
      781 tcgacctcga tcgggtcgag cgcaacatcg cccgcgtaca agcgctgtgt gatcgggccg
      841 gcatggccaa tcgtccgcac atcaagacgc acaaaagccc cgcgctcgcc aaactgcagc
      901 tggccgccgg ggccaagggc atcacctgtc aaaagctcgg cgaagccgag gtgatggccg
      961 atgccggcat cgacgacatc ctcatcagtt acaacctttt gggagaggcg aggatcggcc
     1021 ggctcgctgc gctctcgaag cgggtgaccg tcacggtcgc cgccgacaat cccacggtca
     1081 tcgcggccct ggcaggggcc ggcaagatcg ccggccgccc gcttggcgtc ctgatcgaat
     1141 gcgacaccgg ccgcaagcgc gccggtgttg aggagccccg cgaggccatc gccctggcgc
     1201 gcctcatcaa gcagcatccc gacctcagct tccaaggttt cttgttctat ccaaccgagc
     1261 aatcctggcc gcaaacccag cgcttttacg acgcggcgct cagcggcgtg cgcgaggccg
     1321 gattagagcc ccgcatggtt tcgaccggtg gcacccccaa tctcgtcaac ctgggacggc
     1381 ttgcgggcgc taccgagcac cgcgccggca cctatatctt caacgatcgc atgatgctgg
     1441 cctgtggggc ggcgacccaa gaggattgcg cgctcagcgt ctttgccacc gtggttagcc
     1501 gcgccgcggc cgatcgcggc attctcgatg ccggctccaa gacgctgacc gccgacaccg
     1561 gcggacttga ggacggcttc ggcttgatcc tcgaataccc gcaagccaca atccgtgcct
     1621 tcgccgagga gcatggcttc ctcgacctct cccgcagcaa cgaccgtccc agcgttggcg
     1681 acgtcgtgcg cgtggtgccg aaccatgtct gtgtcgtcgt caacatgttc gatcgcatga
     1741 tcgccgtacg cggcgacagg atcgtggagg tgctgccggt ggcggcccgc ggcaagctga
     1801 gctagcgcgg caagaggctg gggcgttgca gggcaggtgg tgacgcgggc tgccgccgag
     1861 ccgcatggcc cgcaaggcgg cagaatgccc gcgccccggc aacggcgagc gaggcggcgc
     1921 ccccggttgt catggcctgg cggttcggcg acagtagtcg tgcggcagca tttgagcggc
     1981 tcggaggaaa cccatgtcag aagcgctaac gaggtctctc ggcaatttcg tatccggcct
     2041 gcggctcgag gccattcccg cgcctgcctt gagcgtggtt cacacgggtt ttgccgactg
     2101 tgttgccacg ttgatcgcgg gcagcatcga ggcaccaccg cgcattctcc acaaagcgct
     2161 cgccccaccg gccggcgagg caagcctcta cctggtcggg ccgcgtgtgc ctgcaccgga
     2221 cgccgcctgg atcaacggca ccgccgcgca cgcgctcgat tacgacgatg tctccttgcg
     2281 gggtcatcca agcaccgtgc tggtcccggc cattcttgcc gaagccgaag ccatcggcgc
     2341 cagcggcgcc gagatggcca ccgcctacgt cgccggctac gaggtctggg cagagcttgc
     2401 cgggcgcgat ccggatcagc accaccagaa aggctggcac ccgacgggca tcttcggggc
     2461 gatcgcggcg gccgccgcat gtgcctcgct acgcggcctc gacgccgatc agaccacgca
     2521 ggccctcgcg ctcggggcat cgcatagtgg cggactggtc gccaacttcg gcacgatgac
     2581 caaaccctac catgccggca aggccgcgca tgccggggtc atttcggcgc gcttggccgc
     2641 ggcggggttc acggcctccc cggatgccct cgagcatccg ctgggctttc tggccgccgt
     2701 ttcgcccagc ggcgcggccg atcgcgaacg gaaaattgcc gccggacgcg actggcaaat
     2761 cctgagccaa ggcctgtccg tgaaaaaata tcccctgtgc tactgcacgc atcgggccat
     2821 cgacggcatg ctcgatctct tgcgcgagca agaagtcggc gccgcggacg tgaagtcgat
     2881 cacggtctcg accagccggc gtaacgccac cattttgcgc aatgcgcggc cacagaccgg
     2941 gctggaggcc aagttcagca tgcagtttgc gatggcgagc tccatcgtcg ccggccaggt
     3001 cggacttgcc gaattgaccg acggcttcgt ccagcgccag gacgtgcagg ccctgctgga
     3061 caaggtgcgg attgaacccg acgatcggcc cgaccccaag cgctcgggcg ccgcgcccta
     3121 cgatctcgtc gtcattgaaa ccgcgggtgg tcggcgtctg gagagcgcgc gcatcagcga
     3181 tgagcgtggc tcgccgcagc tgccgctgtc gagcgacgag ctgtgggcca agttcgaaag
     3241 ctgcttcagg gtcggcaatg cagccctgcc cgcccgggtg attttcgacg ctctaatgtc
     3301 cctcgagcgc caacctggcg tcgccgcgtt cagcgggttg gcgcaagcgg cctgagctag
     3361 cgcatgcgcc cggtgatggc gcgcgccaga atgcgaccga ggctcgaggc gaccgaacga
     3421 atgaaggatt tggcgatcgc ctcccccacg ctctggcgct ggtagccgct acgccggcgc
     3481 cctggcaccc ggggggcgct ttcgggcccg ttgtgcgggc cagccagcaa ctctttctcc
     3541 gcatccctct tcgccatgga cggctctccg taacggtact cagcggcgcc cacccagcat
     3601 cgcccgcaca atggcgttgc cgatgctgga ggccaccgaa cgcatcatcg atttggcaaa
     3661 ggtttcgccg tagccttggc gccgtccggt gccgccgccc atgagaatgt tgccccaact
     3721 gttggcatcg gcttgctgtt gcgcggccgc ggtcgcggtg cgcttttgca aaatctcgta
     3781 ggcggaatcg cgatcgacgg cctcttcgta cttgccatag atcgggctcg cctcgatctt
     3841 gctcttgcgt tcctcgggcg tgatcggccc gaggcgcccc gacggcggcc ggatcagcgt
     3901 gcgctgaacc atcgaaggct cacccccgcc gtgcaacagg gagaccagtg cctcgcccac
     3961 cttcagctcc tggatgacgc gttcgagatt gagcttcggg ttgggtcgga acgtctcggc
     4021 ggccgcccgg atcccctttt gctcgatggg tgtgaaggca cgcagcgcat gctgcacccg
     4081 gttgccgagc tgagctgaca cggagttggg gacatcgcgc gggttctgcg tgacgtagta
     4141 gaccccgacg cccttcgagc ggatcaggcg ggtcacccgc tccacctgct cgagcagggc
     4201 cttcggggcc tcgctgaaca acagatgcgc ctcgtcgaag aagaacacga gcttgggttt
     4261 atccggatcg cccacctcgg ggagcttgtc gaacagttgc gccaagagcc agagcagaaa
     4321 agtggcgtag agacgtggcg tcttcatcag gcgctcggcc gccagaatat tgacggtgcc
     4381 gcgcccatcg ggcgccgtgc gcatgaagtc tttgatctcg agcgccggct cgccaaaaaa
     4441 gttattggcg ccctgctcct ccaataccag caagcgacgc tgaatggcgc ctaccgaaac
     4501 cgccgagaca ttgccgtact tggtcatcag cgacttgccg cgctggccga catcgttggc
     4561 catcgagcgt aggtccttga gatcgaggag gggcagcttc tcgtccgccg ccacccggaa
     4621 aaggatgttg agcacgccct cctgcgtctc gttgagttcc atcaagcgcg ccagcagcaa
     4681 cggtcccata tcctgcaccg tggcgcggat agggtgcccc tgctcggcaa acagatccca
     4741 gaacacggtc gggaaggccg ccgtcgtcca gtccttcagg ccgatctcct gggcgcgctt
     4801 gctgaggccc tccttcttct cgcccatggc ggaaatgccg gagaggtcgc ccttgatgtc
     4861 ggccgcgaat accggcacgc cggcgttgga aaaaccctcg gccagcactt gcaaggtgac
     4921 ggtcgtgccg gtgccggtgg ccccggtcac gagaccgtgt cggttggcaa gcttcagctc
     4981 gaggtattcg ggcacgacgc tttggccgag gaagatcttg ccctcctgct caaggatttg
     5041 gctctcgacc atggctcgct cctcgataga cgccacgact cacctcaagc gtggcgctgt
     5101 tctcaataca tggtggcgat gcgttggcaa tgctgctgga tctagatcat gcccccgacg
     5161 cgacagcaga actcgagccg ccgcaaggga ttaggttcca gctgcgatcg ctcgttgcgc
     5221 gcgaaattag cggaaaaacg gggcggcgac cacccggccc gccaacccgc aggccgcgtc
     5281 gcgcgccact gaggccagcc gtcgcggcgc acgcgatacc gccccaatgt cccgccctcc
     5341 gatgcgcgcc cgagccgggc cgtggcgctt agcgcgcgcc gctggattga tcacgtctgc
     5401 gggatcttat agatcccccc tggcggttga gcgctaggga tcggcaggga ggcgcgcatg
     5461 gatctgagca cggaaagcct attccaggtg ttggagagcc gcggcggcgg gcgctatggc
     5521 ctgtcggcgg tgacgcaaaa ggagcatgcc ctgcaagcgg ccgcgcttgc ggccgaccgc
     5581 ggcctcggtg atcagctggt gatcgcggcg ctgtttcatg acgtcggcca cctcctcgtc
     5641 ggcgaggacg tcgacctggc cgaagcgggc atcgacgacc tgcacgagga gacgagcgcg
     5701 aaggcactcg aggccttgtt tggcaaggac gttgccgagc ccgtgcggct gcacgtggcc
     5761 gccaagcgct atttgtgcgc cgtcaacccc gcctactatg acaagctttc tgaggactcg
     5821 cggcgctcgc tggccctgca ggggggcccg atgtcacccg gcgaggtcgc cgctttcgac
     5881 gggttggcgc atcgggccgc cgcgctcgct ttgcgcatca tcgacgacga ggcaaaggtt
     5941 gagggtctga aaacgccacc cctggagagc taccgtgcgc ttgccgcccg gctgcaaggg
     6001 ggagcggcat gaggccgcgc caggtgcaag tcctgcggcg ctgagttgcg gcgcaccgcc
     6061 gcgggaaagc gcgcagttgc ctggaaaatt cggcgctgat cgcgcccgtg aagcctgcac
     6121 agcacgaagc aagcggcggg tcaacgtcga gcgcgctttt gtgacagttt gataaatcgg
     6181 tagtcccacc gatgtacgcg ggccgcgcca tccccgcatg ctgcaaatcc cctcagcttg
     6241 caacaggtgt cggcatgcgt ctcgcctcgc ttcgcgatca gcaccagccc gtcccttacc
     6301 gggaagagga aaaagcaatg gacgcggagc gtcccgcaac gcaggagctc tgcgacgcca
     6361 tcctcaacgc cttggggcgc gacgagcgcc tgcgccgcga ctacgtgcgc cgccgctggc
     6421 gaggcctgat cgtagaggcc cgcgccatca gggctgcggc cgcgccgggt gagggctagc
     6481 atcgccagcg catttcggcg ttgcgcgcga gcccttcgat ccgcggcggc ctgagcgctc
     6541 cggatccggg ctcgccagcg cctttgcccc cggcactctc aagcgcgcgg cggataacgc
     6601 tcgaactcgg gcagttcatg cgcgcgctgc atccagccga tgcgatcggc gacttggatg
     6661 tgtgcctgcg ccggaaccgc atcggggtcg tcataggtgg cgctctgaat atcgagcagc
     6721 cccggcagca cgacatcgtt gctgaaaaaa aggccggttc cgcaatccgc gcaaaaatag
     6781 cgccgcccat cttgcgacga ggcgtagact ttggggcttc cctttgtgac cgtgacgcgc
     6841 tcgcgcggat acatcgtcca acccaccatg ggcgcaccgg catgtcgtcg acagtccgtg
     6901 cagtggcaga gcgcgtggtt gagcgcctca ccctccgcct ggtagcgaat ggcgccacag
     6961 tgacagccgc cagtgattgc catgctttat ccttccgcaa cgaccgttgc ccgcgcagcc
     7021 ccatgctcct cgacatgaag ggcctgcgca agccacctcc gccggcgtag catgccctcg
     7081 ggccgcgcgc gcgcgtgtgc gagcatcgct cccagcccgc agggtcaagc cggcgcccat
     7141 cgccgcccgg ctgcgccggg tgccggcgat cgacttgggc tcgacccttt cgttcgcacc
     7201 agctcggcgt ctgagcgggg acgccgccca cgccagcggt tcggcgctaa gatcggtttg
     7261 gccggcggca ggaatggtct accaatgggc tggacctgcc aacacacccc cggaggcgcc
     7321 ttagcgatca ccatgaccga cgacctcgaa ccgatcagca cggagttcga agcggcctcg
     7381 cgcgaggctt ggctcaaact cgtcgacaag gtgctgaaag gcggctcgtt cgagcgccag
     7441 ctcgtatcgg agaccgccga tgggttgcgg atcgatccgc tctttacgcg cagcgaggcg
     7501 gcgcccgtca actcggtgca ggtgggtcgg cccgccgccg ccggccatcg gtgggatatt
     7561 cgccagcggc acggagaacc cgaccccaag ctctgcaatg gagccatcct cgaggacctg
     7621 gaaggcgggg tcaactcgct gctactgcag atcgaggcac cggggcaatg gggtcttggc
     7681 tatggcgccg agccgctggc acgtgcgctc aggggcgtgc gcttggagca agtgccgatt
     7741 gcactcgatg cgcgcgaaaa caccctcgac gcggccggta gcctgattga gatttggcgc
     7801 gaggcaggtg tcggggagcg ccagcggcgg ggggccttca actatgatcc gctcggcgtg
     7861 ctggccaaaa ccgggacgct ctactacgcg cccgatcgcg cctgcgaaat cgccgccaag
     7921 ttcgcctccg atcagcaagc catgcccaac gtcacggccc tattggccga tgcgcggccc
     7981 taccacgagg cgggcgccgg ggaggcgcaa gaattggcgg cgctgctggc aacgctggtc
     8041 gcctacttgc gcgcttgcga ggcgggcggt cttccgccac gtgccgcttt cggcaaaatc
     8101 ggcttcgccg tggcgatcga tgccgatctc tttgtcacca tcgccaagct ccgcgccgca
     8161 cgcagcctcc tggcgcgggt tgcgcaggct tgcggtgtcc cggaggcggc ggaggcgctg
     8221 acgctaaacg cctccacctc cgaacgcatg ctggccaaac gcgatccgtg ggtgaacatg
     8281 ctgcgcgcga cggtggcatg cgcaggcgct gccctcggcg gcgcagacag catcagcgtg
     8341 ctgccgttca cctgggcgtt gggcaagccc gaccgcttcg cgctacgcat cgcgcgcaac
     8401 acacacctgg tgttgcagga ggagagtgcg ctgggacgcg tcgcggatcc ggcgcatggt
     8461 gcctggttca tggaacagct gacgaccgag ctggccgaga aggcctgggc gctctttcag
     8521 gccatcgaac atgcgggcgg tatggcgcgt gcactccaat ccggtttcct tgcggccgag
     8581 gtcagcaagg ttgcgcgtgc acgcagcggc cgcatcgcgc aggctcggct ggagctgacg
     8641 ggggtctcgg cctttccccg gctcggtgac gacggggtga gcgtggcgcc gcaccccatg
     8701 gctccccccg ttgtcagggg cgggacctcg gccgagccct tcccgttgcg gcggctggcc
     8761 gaacccttcg agcgcctgcg cgacgccgcc gatcgccacc tggctcgcac cggccagcgc
     8821 ccacaggtgt ttctggcttc gctgggcgag gcggccgttc actcggggcg caccacctgg
     8881 atgcagaact tcttggccgc cggcggcatt gaggccgtgc cgagcccgcc gctgcataat
     8941 tctgccgctg cgggcaaggc ctttgccgag agcgggacct gcgccgtctc gatctgctcc
     9001 tcggatgccg tctatgccga attggccgag gcaacagccg gcgtgctgaa agccgcgggt
     9061 gccaagcagg tgctcttggc gggtcggccc aagggccagg aggaggcgct gcgcgcggcc
     9121 ggcgtcgatg agtttgtgtt tgccggcggc gatgctctgg ccacgctcaa ccgcctacac
     9181 gaggtgctcg atgtcacatc ctgagccgtc gcaaacgcct cgtcataccg gcggttgcca
     9241 gtgcggtgcg gtccgctttg ccgtctatgc cgatcccctc aaaatcggca tctgccactg
     9301 ccgcatgtgc cagaaggcgg tggctggccc atttgccgtg ctggccgagg tgcccggcgc
     9361 gcagtttgcc tggacacgcg gccggccggc cacctttcaa tcctccacgc gcgccgtgcg
     9421 cgatttctgc cccgcctgcg gcacgccctt gagctaccgt catcccggcg gcccgatcat
     9481 cgagctgctc accggcgcgt tcgatcaacc tgcccgcctt cccccgacct atgccgtcgg
     9541 caccgaatcc atgctcgcct ggctcgccga tattgctaag ctgcccggca aaaccacggc
     9601 aaacaatctt gcttcgcagc cgaccgccga catcgtcggc ttccagcacc ctgatcacga
     9661 cacggatcgc acctggcagc cgccgcgtcg cgcatcgccg gatgcgtgag ggtcactacc
     9721 ggccggcaac accgcgcgcg gcgagcgacg gggtgccaac tcggtgattc gggccgagcc
     9781 cgccgctgtc ggcaccgccg caaagactag ggaagcctcg cgcccgccat gagaaccctc
     9841 ttcgactttg ccgccaagcg cgcggagctc tcgcccgacg cgctggcctt cgcggaattc
     9901 gagccggaga gtaagctctg cttcggcgag ttcaacgcgc gcgccgagcg cggcgccagc
     9961 ctgctcgagc gtcagttcgg cagcggtgat cgcatcgccg tgctctgtca taattgcacg
    10021 gggttcttcg agctgctctt cgcctgcggc aaaatcggtg tcatcctggt gccgctcaat
    10081 tggcggcaaa ccccgaccga gttaaccccc atcctgctcg actgcggggc gaacatgctc
    10141 ctgcacgatg ggccgacggc gccgctggcc gaggcgttgg cggccagcac cggcgtgcgc
    10201 ctatgcacgt tcgccgccta cgcgcagatg cgcgcagaaa cgagcccaaa tccgcgccgc
    10261 gagacggcct ggcccagcga ccgcatctgg tatctgctct atacctcggg caccacgggg
    10321 cgacccaagg ccgtgatcca gacggtgggc atggcattgg ccaacgccat caatgtgcag
    10381 caggcgacgg cactgagttc gctcgaccgc accgtcaact tcctgccgct gttccacacc
    10441 gccggcatca acttgcacac gttgcccgtc ttcatcgcgg gcggcagcag cacggtcttg
    10501 gccaagttcg aggttgatcc gcttttcgat ctgattgcgg ccggccgcgt cagtgttttc
    10561 ttcggcgtgc cgagcatcta ccaggcgctg agcctctcct cgcgctttgc cggg
//
LOCUS       DASTMT010000086         1725 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_703570, whole genome shotgun sequence.
ACCESSION   DASTMT010000086 DASTMT010000000
VERSION     DASTMT010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 1725)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1725)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1725
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_703570"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1725
                     /locus_tag="VFR00_01785"
     CDS             <1..>1725
                     /locus_tag="VFR00_01785"
                     /EC_number="2.2.1.2"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012752672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional transaldolase/phosoglucose
                     isomerase"
                     /protein_id="HEU0158008.1"
                     /translation="LGGLGQSIWLDFLSRNLISSGDLERLVKHDGLAGITSNPTIFEK
                     AIGHGTDYDASLQAAAADGERDANALYERLAIEDIQHAAAVLRPLYDRSDGHDGFVSL
                     EVSPYLAMDTKATIAEARRLWRTVARPNLMIKVPATSAGLPAIRELTGEAINVNITLL
                     FAQSTYAEVVAAYLSGLEHAAAAGKDLSRIASVASFFVSRIDTAVDKRIETMLATAPA
                     GEREQLARLRGTIAIANAKLAYQIYQRQFAGGRWQALATKGARVQRLLWASTGVKNPN
                     YRDTLYVEELIAAETINTMPPATLDAFRDHGKVAPTLETGLEQAKEQLAALARCGIAL
                     EQITSDLVEDGVKLFDEAFDKLLTAIAHKRQGLLGARLNGQSASLPKELDAAVTAELE
                     AWRQAGNIRRLWRGDASLWTGNDEDRWLGWLGIVGEQLARSQALRSVAADIREPGFSH
                     VVLLGMGGSSLGPEVLAKTFGAQAGRPQLLVLDSTDPAQIRAVEAELDLARTLFLVSS
                     KSGTTLEPNILKAYFLQRLTATLGADQAGSRFFAITDPHSQLAEVAGRERFRGLAFGK
                     PSIGGRYSV"
BASE COUNT          293 a          594 c          557 g          281 t
ORIGIN      
        1 ctcggcggcc tcgggcagtc aatctggctc gattttctgt cgcggaactt gatcagcagt
       61 ggcgaccttg agcgcctcgt caaacatgat ggcctcgccg gcattacctc aaaccctacg
      121 attttcgaga aggcgatcgg acacggcacg gactacgacg cctctttgca ggccgccgcg
      181 gccgatggcg agcgggatgc caatgcgctc tacgagcggc tggcgatcga ggacatccag
      241 catgccgccg ccgtgttgcg gcccctctac gatcgcagcg acgggcacga cggcttcgtc
      301 agcctggagg tgtcgccgta cctggcgatg gataccaagg ccaccattgc agaggcgcgc
      361 cggctgtggc gcaccgtcgc ccgtcccaac ctaatgatca aggtgccggc gacgtcggcg
      421 ggccttcctg ccatccgcga gctcaccggc gaggcgatca acgtcaatat cacgctgctg
      481 tttgcgcaga gcacctacgc ggaggtcgtt gcggcctacc tgtccggcct cgagcatgcc
      541 gccgccgcgg gcaaggacct cagccgcatc gccagcgtcg ccagcttctt cgtcagccgc
      601 atcgatacgg ccgtcgacaa gcggatcgag acgatgctgg cgacggcgcc cgccggcgag
      661 cgcgagcagc tggcgcggtt gcgcggcacc attgccatcg ccaacgccaa gcttgcctat
      721 cagatctacc aacgccaatt cgccggcggc cgctggcagg ctcttgcaac gaaaggcgca
      781 cgcgtgcagc gtctgctgtg ggcgagcacg ggcgtcaaga acccgaacta ccgcgacacc
      841 ctctacgtcg aggagctgat cgcggccgag accatcaaca ccatgccgcc ggcgacgctc
      901 gatgcctttc gcgaccacgg caaggtggcg ccgaccctcg aaaccggtct cgagcaggca
      961 aaggagcaac tcgccgcgct cgcccgttgc ggcattgccc tcgagcaaat aacgagcgac
     1021 ctggtcgaag acggcgtcaa gctgtttgac gaggccttcg ataagctgct caccgccatt
     1081 gcccacaagc gccagggcct cctcggggcg cggctcaatg gccagtccgc cagtctgccg
     1141 aaggagctag atgccgccgt caccgccgaa ctcgaggcct ggcggcaggc gggcaacatc
     1201 cgtcgcttgt ggcgtggcga tgccagcctg tggaccggca acgatgagga ccgctggctt
     1261 ggatggctcg gcatcgtcgg cgagcagctg gcgcgcagcc aagccttgcg cagcgtggcg
     1321 gcggacattc gcgagccggg cttcagccac gtcgtgctgt tgggaatggg tggctcgagc
     1381 ctcggcccgg aggtgttggc gaagactttt ggcgcccagg ccggccggcc gcagctgctg
     1441 gtcctcgatt ccaccgatcc cgctcagatc cgcgccgtcg aagccgagct cgatctggcg
     1501 cgcaccctgt tcctggtctc cagcaagtcg ggcaccacgc tggagcccaa catcctcaag
     1561 gcctatttcc tgcagcggct aacggcaacg ttgggagccg accaggcggg ttcgcgtttt
     1621 tttgccatca ccgacccaca ctcgcagctg gcggaggtcg ccgggcgcga gcggttccgc
     1681 ggcctcgcct ttggtaagcc cagcatcggc gggcgctact ccgtg
//
LOCUS       DASTMT010000087         7816 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_1360005, whole genome shotgun sequence.
ACCESSION   DASTMT010000087 DASTMT010000000
VERSION     DASTMT010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 7816)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7816)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7816
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1360005"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..538)
                     /locus_tag="VFR00_01790"
     CDS             complement(<1..538)
                     /locus_tag="VFR00_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006021697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU0158009.1"
                     /translation="MGDVSVRDPGASDTERQRGAREAGRGLAGVLPPFLAKPNPHFVL
                     AAYTATTFLSALLLFSVQPMFAKMVLPVLGGSPSVWAVAIFFFQAALLIGYCYAHLLI
                     AKASALSTGVIHLAVCCVAFLALPIGLPSGWTEPPAGEPYLWQIGLFTVAIGLPFLAV
                     AANAPLLQAWFARTGHQQA"
     gene            840..1271
                     /gene="ruvX"
                     /locus_tag="VFR00_01795"
     CDS             840..1271
                     /gene="ruvX"
                     /locus_tag="VFR00_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003610880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEU0158010.1"
                     /translation="MLPAGARLLGIDVGSKTLGLALSDVTRTIASGLTTLKRDKFSLD
                     VARLTELVTANAVGGLVIGLPQNLDGSVGARAQSTRAFARNVAKRMALPILLWDERFT
                     TAEAERVLIAADLSRRRRAAVIDKVAATLILQSALDRMQRV"
     gene            1783..2226
                     /locus_tag="VFR00_01800"
     CDS             1783..2226
                     /locus_tag="VFR00_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013950226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEU0158011.1"
                     /translation="MKNNHRSGPLEASALHLLHRAGQRAEELFTNETGKVDLTPRLYA
                     VLACVAHNPDISQTGLVEQTGVDRSTLADIVRRLVKKGLLHRKRTRRDARMYAVRLTA
                     KGQSALGAVKPVASRVDQRILSALKTDQRGDFIEALGEIVKALHS"
     gene            complement(2275..3945)
                     /locus_tag="VFR00_01805"
     CDS             complement(2275..3945)
                     /locus_tag="VFR00_01805"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011927349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HEU0158012.1"
                     /translation="MLEPFQTHEVFNQSPPFQDVNLFTSDRPLMEAVKREGAAGALER
                     LGQFGATCGSAEALEQARLANENAPRLMSFDSKGARLDLVEYHPAYHVLMQISMAQGL
                     HASVWEHLAEPGGGGRRPGASVERSAGCYLANQMEAGHQCPITMTNAAVPTLLMAPEL
                     AAQWLPKILARTYDKALRPLAQKRSVTIGMGMTEKQGGTDVRANTTRAEPVGGRGPGA
                     EYLITGHKWFMSAPMSDAFLVLAQAPGGLSCFLLPGVLPDGKRNALRFQRLKDKLGNR
                     SNASSEVEFQAAHGWLIGQEGRGVANIMEMVTLTRLDCAVASAGLMRLALANAIHHCR
                     HRRVFQKHLIDQPLMQHVLADMALDVEAATALAFRLARAFDRAEEESEAAWRRLMTPV
                     TKYWVCKMGPALGYEALECLGGNGYVEAGLAARLYRELPLNAIWEGSGNVMALDLLRV
                     LEREPETFAAVLDALAALAGPEPTLRAELERLHALSQDPAGLMAQGRDLIESLARLAA
                     GALLRAEAPAAVGDAFLGTRFSRASGRSYGHGLAGADTRAIVERALAS"
     gene            4084..5034
                     /locus_tag="VFR00_01810"
     CDS             4084..5034
                     /locus_tag="VFR00_01810"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007603315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEU0158013.1"
                     /translation="MTSAIHPATFVRRHLLTCEGLKAGEISALLDLADKAAEGNRQVI
                     KKRDVLRGRTLINLFFEASTRTQSSFELAGKRLSADVMNMNVSTSSVAKGETLIDTAM
                     TLNAMRPDILVVRHHAAGAVELLAQKVDCSVINAGDGAHQHPTQALLDALTIRRAKGR
                     IAGLTVAICGDILHSRVARSNINVLNTMGARVRVVAPSTLLPAHTDRLGAEVFTDMNA
                     GLEDADIVMMLRLQKERMQGSYVPSTREYFHFFGLDHEKLARAKPDALIMHPGPMNRG
                     VEIASTIADHSRSVIREQVEMGVAVRMAVLEAMARQLPNH"
     gene            5048..6370
                     /gene="pyrC"
                     /locus_tag="VFR00_01815"
     CDS             5048..6370
                     /gene="pyrC"
                     /locus_tag="VFR00_01815"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020178310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEU0158014.1"
                     /translation="MKKSDPTPDATAFLNARLIDPASGKDEPGGLLVRGGVIADLGNH
                     LRRNAPDGAAVIDCKGHVLCPGIIDMQVFTGEPGQEHRETLKTASQAAAAGGVTTMVV
                     MPDTQPVIDQVALVDFIQRRARDNAVVNVYIMAAMTRGLNGQDMTEIGLLKRAGAIAF
                     TNGKSSVGNTRVMHNVLLYAKDFGALIAHHTEDPHLSDGAVMNAGEVATRLGLPAVHK
                     AAETIVLERDVRLVEITGGRYHASTISCAESLAVIRAAKARKLPVTCGVSVNHLTLNE
                     NDIGPYRTFFRLRPPLRSEEDRAGMVRGIAAGDIDVIVSSHDPQDADVKRHPFAEAAD
                     GAIGLETLLAAALRVHHSGEVGLIPLLKAMTANPAKLLGLASGRLQKGAPADLIVVDL
                     GLPWVVDKVQLKARSKNSPFDESKMQGRVLNTMVAGALVYQYAGMDRS"
     gene            6412..7017
                     /gene="plsY"
                     /locus_tag="VFR00_01820"
     CDS             6412..7017
                     /gene="plsY"
                     /locus_tag="VFR00_01820"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013895329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEU0158015.1"
                     /translation="MLYGLLALLAGYLLGSIPFGLLLTRWAGLGDVRRIGSGNIGATN
                     VLRTGHRGLAAATLALDLLKGTLAVLIVYALAKRAVGFEAYRLTYLAGLGAVLGHLFP
                     LWLGFHGGKGIATFIGVLLGVHWPAAAVFGLIWLGVAAVWRYSSLAALCGTAGVIVYY
                     LIFGQAGLPYVLVMAALVTLKHHANIRRLLAGQESRIGAKP"
     gene            7427..>7816
                     /locus_tag="VFR00_01825"
     CDS             7427..>7816
                     /locus_tag="VFR00_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006924417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HEU0158016.1"
                     /translation="MNVVIVESVAKAKAINKYLGSSYKVLASYGHVRDLPSKDGSVEP
                     DKDFAMHWDVDGRSSKVMREIADAVKKADKLILATDPDREGEAISWHILRILEDKKAL
                     KKGVPVERVTFNAVTKASVLEAFTAPRA"
BASE COUNT         1337 a         2665 c         2529 g         1285 t
ORIGIN      
        1 cggcttgttg atggcccgtg cgcgcgaacc acgcctgcaa caggggcgcg ttggcggcca
       61 cggcgaggaa gggcaggccg atcgccaccg tgaagaggcc gatttgccac aggtagggct
      121 cgcccgccgg cggctcggtc cagccgctcg gcagaccgat cggcagcgcc aggaaggcga
      181 cgcagcagac ggcgagatgg atgacccccg tgctgagcgc cgaggccttg gcaatcagca
      241 aatgcgcata gcagtagcca atcaggagcg cggcttgaaa gaaaaagatc gcgaccgccc
      301 agaccgaggg tgaaccgccg agcacgggca acaccatctt ggcgaacatc ggctgcacgg
      361 agaacagcaa cagcgccgac aggaaggtgg tggcggtata ggccgcgagc acgaaatgcg
      421 gattgggctt ggccaggaag gggggcagga cgccggcgag gccacgcccg gcctcgcgcg
      481 ccccgcgctg gcgctcggtg tcgctcgcgc cggggtctcg cacgctgaca tcgcccataa
      541 gctctctcct gtcttggcgc cggccaaggg caagcgccgc ggcactatat ctcccgcgcc
      601 gctgcccatc aaaacgatcc cggcggcggt ttggtgcccg cgcgtgggcg ccctagcgtt
      661 tcggcctgaa gcctgcgctc gaattgtggc ccggcgccgg aggctggtga tttgcgcgcc
      721 gtgagcgggc gcgctaagag gccaagtgaa cccaataagc ccaaggcgct ccgcacgtgg
      781 cggctcagga cacggatcgg cccgccgccc tcctcgagga tgcggacgcg tttgagcgtc
      841 tgctgccggc cggcgcgcgc ctgctcggta tcgatgtcgg cagcaaaacc ttgggccttg
      901 cgctttccga cgtcacgcgt accatcgcct cgggactgac gaccctcaaa cgtgacaaat
      961 tctccctcga cgtggcacgc ctgaccgaat tggtcacggc gaacgcggtc ggcggcctcg
     1021 tcatcggact gccgcaaaac ctcgacggca gcgtgggcgc gcgcgcgcaa tcgacgcgcg
     1081 cttttgcgcg caacgtggca aagcgcatgg ccctgccgat cctgctctgg gatgaacgtt
     1141 tcaccaccgc ggaagcggag cgggtgttga tcgcggccga tctctcgcgc cgccgccgcg
     1201 ccgccgtgat cgacaaggtt gccgcgaccc tcatcctgca atcggcgctc gaccgcatgc
     1261 agcgcgtctg agcccgccgc ggcccagcca agtttgccgc aagcttgatc ggtgcggtca
     1321 agcgcacaat tgtggacgct ggccttttgg cccgcggctc tctacggctg gcgcaagcgg
     1381 cagagccaac gcggaaatgt tgcttgcgcc ggcgaccgcg gcgcgcgcaa cggggatcat
     1441 caccgtgcaa cgattacgtt cgtgaattgc tcgtcctgcg gaggatcacc ggcgcccgcg
     1501 ctgcggccag caccccgcat ggcccgacca agcgcggccg ctttgcgtca gtgccgcgac
     1561 cttttcgcgc gtccaatacc acttttcgtt agcgctgggt ttgctagcgt attctaattg
     1621 agccgtcatc ggtcgaattt ttataggccg acggataaag ccgctggggt ccgtctggat
     1681 cattgcggta tcgcacacta cgtacagagc caggtcggcg cgagcgccca acagctgctc
     1741 tggcttttct catcaatatt gagtgaggtg gatatccaag ccatgaagaa caaccatcgt
     1801 tcaggcccgc ttgaggcgtc ggccctgcac ctcctgcacc gtgctggtca gcgcgcagag
     1861 gaactcttca ctaacgaaac gggcaaagtc gatctaaccc cgcgcctgta tgccgttctc
     1921 gcctgcgtcg cccacaaccc cgacatttcg cagacggggc tggtcgaaca aacgggggtc
     1981 gaccggtcca cgcttgccga catcgtgcgc cggttggtga agaaggggct tttgcaccgc
     2041 aagcgcaccc ggcgcgacgc gcgcatgtat gccgtgcggc tgaccgccaa ggggcagtcg
     2101 gcgctcggcg ccgtcaaacc ggtcgcctcg cgcgtcgacc agcgcatcct gtccgccctg
     2161 aagaccgatc agcgcggcga ctttatcgag gcgctgggcg agatcgtcaa agcgctgcac
     2221 tcctaaggat cagccagggg gtgggacgct aagcttaagc ttgaggcgtt ggcgctaaga
     2281 agccaacgcc cgctcgacga tggcccgggt gtcggcgccc gccaggccgt gcccatagct
     2341 gcgcccgctg gcgcgggaaa agcgcgtgcc caggaaggca tcgcccaccg cggcgggggc
     2401 ctccgcgcgc aacagcgccc cggcggccaa gcgcgccagg ctctcaatca ggtcgcggcc
     2461 ctgcgccatc agccccgccg gatcctggct gagggcgtgc aagcgctcaa gctcggcccg
     2521 caaggtcggc tccgggccgg ccagcgccgc cagcgcgtcc agcactgcgg cgaaggtctc
     2581 gggctcgcgc tcaagcacgc gcaacaggtc gagcgccatg acgttgccgg agccttccca
     2641 gatggcgttg agcggcagct cccggtagag gcgcgcggcc aagcctgctt ccacgtagcc
     2701 gttgccgccc aagcactcga gcgcctcata gccaagcgcg ggccccatct tgcacaccca
     2761 gtatttggtc accggcgtca tcagccggcg ccaggcggcc tcgctttcct cctcggcgcg
     2821 gtcgaaggcg cgtgccaggc gaaaggcgag cgcggtggca gcttccacgt cgagcgccat
     2881 gtcggccaag acatgctgca tcagcggctg atcgatgaga tgcttctgga atacgcggcg
     2941 gtgccggcaa tggtggatgg cgttggcgag cgccaggcgc atcagccccg ccgaggcgac
     3001 cgcacagtcg agccgcgtca gggtcaccat ctccatgatg ttggcaacgc cgcgcccttc
     3061 ctggccgatc agccagccgt gcgcggcctg aaactccact tcggaggagg cattcgagcg
     3121 gttgccgagc ttgtccttga ggcgctggaa ccgcagcgcg ttacgcttgc cgtcgggcaa
     3181 aacgcccggc agcaggaagc agctgagccc gccaggcgct tgcgccagca cgaggaaggc
     3241 atccgacatc ggcgccgaca tgaaccattt atgccccgtg atcaggtatt cggcgccggg
     3301 accgcgcccg ccgaccggct cggcccgcgt ggtattggcg cgcacgtcgg tgccgccctg
     3361 cttttccgtc atacccatgc cgatggtgac gctgcgcttc tgagccagcg gccgcagcgc
     3421 cttgtcgtag gtgcgcgcca ggatcttggg taaccactgg gcggccagtt ccggcgccat
     3481 cagcagcgtc ggcaccgccg cgttcgtcat cgtgatcgga cactggtggc ccgcttccat
     3541 ctggttggcg aggtaacagc ccgccgagcg ctcgacgctg gcgcccgggc gcctgccgcc
     3601 gccgcccggc tcggccagat gctcccagac ggaggcgtgc agaccctggg ccatgctgat
     3661 ctgcatcaag acgtgatagg ccgggtgata ctccaccaag tcgagcctgg cgcccttgct
     3721 gtcgaaactc atgagccgcg gcgcattttc gttggcgagc cgcgcctgct ccagggcctc
     3781 cgcgctgcca caggtcgccc cgaattggcc gagccgttcg agcgcgcccg ccgcaccctc
     3841 ccgcttgacc gcctccatca gcggccgatc gctggtgaac aaattcacgt cttggaaggg
     3901 cgggctctgg ttgaaaacct catgcgtttg gaacggctcg agcatgcgct gcgtgctcct
     3961 tgcaggaaag cctcagccta gcaactggca cgggtcggcg cagcccatct gcgaccttct
     4021 ccaccgctcg caaccgagcg cggcttgcga gcctggcgga gtcggcgtat aggaccgcct
     4081 ttaatgacca gcgccattca tcccgccacc ttcgtccgcc gccatctgtt gacctgtgag
     4141 ggcctcaagg ccggcgaaat cagcgcgctt ctggatttgg ccgacaaggc cgcggagggc
     4201 aaccgccagg tcatcaaaaa gcgcgacgtg ttgcgcgggc gaacgctgat caatctgttc
     4261 ttcgaagcct cgacgcgcac gcagagctcc tttgagctgg cgggaaagcg cctgagcgcc
     4321 gacgtcatga acatgaacgt ttcgacctcc tcggtggcca agggcgagac gctgatcgac
     4381 acggccatga cgctcaacgc catgcggccg gatatcctgg tggtgcgcca ccacgccgcg
     4441 ggggccgtcg aactgttggc ccagaaggtc gactgctcgg tgatcaacgc cggcgacggg
     4501 gcgcatcagc atccgaccca agcgctgctc gacgcgctca ccattcgccg cgccaagggc
     4561 cgcatcgccg ggctgacggt ggcgatttgt ggcgacatcc tgcactcgcg cgtggcgcgt
     4621 tccaacatca acgtgctcaa caccatgggc gcccgcgtcc gcgtggtggc accctcgacg
     4681 ctgctgccgg cgcacaccga tcggctcggc gcggaggtct tcaccgacat gaatgcgggc
     4741 ctggaggatg ccgacatcgt catgatgctg cggctgcaga aggagcgcat gcaaggctcc
     4801 tacgtgccct ctacgcgcga atacttccac ttcttcggtc tcgaccacga aaagctggcc
     4861 cgcgccaagc ccgacgcctt gatcatgcat ccaggtccca tgaaccgcgg cgtcgagatc
     4921 gcctccacca ttgccgacca ctcccgcagc gtcattcgcg agcaggtcga aatgggcgtg
     4981 gcggtccgca tggccgtgct ggaggccatg gcgcgtcagc tgcccaacca ttgagaagtg
     5041 ccgcgtcatg aagaaatccg atccgacccc ggacgcgacc gcgtttctca acgcccggct
     5101 gatcgatcct gcgtcgggca aggatgagcc aggcggcctg ttggtgcggg gcggtgtgat
     5161 tgccgatctc ggcaaccact tgcgccgcaa cgctcccgac ggggcggcgg tgatcgactg
     5221 caagggccac gtgctctgtc caggcatcat cgacatgcag gtgttcaccg gcgagcccgg
     5281 gcaggagcac cgcgagacct tgaagacggc gagccaggcg gcggcggccg gcggcgtcac
     5341 caccatggtt gtcatgccgg atacgcagcc cgtgatcgac caagtggcct tggtcgactt
     5401 catccagcgc cgggcgcgcg acaatgccgt cgtcaacgtt tacatcatgg cggccatgac
     5461 gcgcggcctc aatggccagg acatgaccga aatcggcctc ttgaagcggg ccggggccat
     5521 tgccttcacc aacggcaaat ccagcgtggg caacacgcgg gtgatgcaca acgtgctgct
     5581 ctacgccaag gacttcgggg cgctgatcgc ccatcacacc gaggatcccc acctgagcga
     5641 cggcgcggtg atgaatgccg gggaggtcgc cacgcgcctc ggcctgccgg ccgtgcacaa
     5701 ggccgccgaa acgattgtgc tcgaacgcga cgtgcggctg gtcgagatca ccggtgggcg
     5761 ctaccacgcc tccaccatca gttgcgccga aagcctggcc gtgatccgcg ccgccaaagc
     5821 tcgcaaactg ccggtgacct gcggcgtctc cgtcaatcac ctcaccctca acgagaacga
     5881 catcggcccc tatcgcacct ttttccgctt gcggccgccg ctgcgctccg aggaggaccg
     5941 ggcagggatg gtgcgcggca ttgccgcggg cgacatcgac gtcatcgtct cgagccatga
     6001 cccccaagac gccgacgtca agcggcatcc ctttgcggag gcagccgacg gcgccattgg
     6061 cctggaaacc ctgctcgccg cggccttgcg cgtgcatcat agcggcgagg tcggtctcat
     6121 tcccttgctg aaggcgatga ccgccaatcc tgccaagctg ttgggccttg cctcgggccg
     6181 gctgcagaag ggcgcaccgg cagacttgat cgtggtcgat ctcggtttgc cctgggtggt
     6241 cgacaaggtg caactcaagg cgcgctcgaa gaactccccc ttcgatgaaa gcaagatgca
     6301 aggccgcgtt ctcaacacca tggttgcggg cgcccttgtc taccaatacg ctggcatgga
     6361 ccgcagctga cgctggtgtt gagccgagcc acggggatgg gcaccgcctt catactctac
     6421 ggcctgctcg cgctgctcgc gggttatctg ttggggtcga tcccgttcgg attgctgttg
     6481 acgcgctggg ctgggctcgg tgacgtgcgc cgcatcggct cgggtaatat cggcgccacc
     6541 aacgtgttgc gcaccggcca tcgcggcctg gcggcggcca ccctggcgct cgacctcttg
     6601 aaggggaccc tggccgtcct gatcgtctac gcgctcgcca agcgcgcggt gggcttcgag
     6661 gcctatcggc tcacctacct ggccgggcta ggcgcggtgt tggggcacct tttcccgctg
     6721 tggctgggct ttcacggcgg caagggtatc gccaccttca tcggcgtgct gttgggggtg
     6781 cactggcccg ccgccgccgt cttcggcctg atctggctcg gcgtcgccgc cgtctggcgc
     6841 tactcctccc tggccgcgct ctgcggcacg gcgggcgtca tcgtctacta cctgattttc
     6901 ggtcaggcgg ggctccccta cgtgctggta atggcggcgc tcgtcaccct gaaacaccac
     6961 gccaacatcc gccgcttgct ggcaggtcag gaaagccgca tcggggcgaa gccctgaacg
     7021 gcggctgcgc ctcgctgggt ccgcggctct cccgttgcgc tgatcgcgcc gagccgtctg
     7081 accgagggac agcgccgggg gcagcaaacc cggcacgcgc ggccgcggcc aagcagcccc
     7141 tctttctcga ggcttgagcg ctgccggggc gggcgcgcga cccgggccca agcaaaatcc
     7201 ccacaaaatc gcgcaatcat tccccgcgag aaccgcaaat atccaacaaa agggctaaaa
     7261 aactacgtcc tggttgatta tcgaccgaaa atagcgcccg ccgggcgttt gacacgcccg
     7321 gcggcatcca ccatgttctc gccgcccatc gggcaaagcg tagcaacagc tgaggatcgc
     7381 gcggccgccc agagggcctg ggtcggcgct gccgcagaga cctttcatga acgttgtcat
     7441 tgttgaatcg gtcgccaagg ccaaggcgat caataaatat ctcggttcga gctataaggt
     7501 tctggcctct tacgggcacg tgcgcgacct gccctccaag gacggctcgg tggagccgga
     7561 caaggacttt gccatgcatt gggacgtcga tggcaggtcc agcaaggtca tgcgcgagat
     7621 cgccgatgcc gtcaaaaagg ccgacaagct gatcctggcc accgaccccg atcgcgaggg
     7681 cgaggccatt tcctggcata tcctgcgcat cctggaggac aagaaagccc tgaaaaaggg
     7741 tgtccccgtc gagcgcgtca ccttcaatgc cgtcaccaag gcctccgtgc tcgaggcgtt
     7801 cacggccccg cgcgcc
//
LOCUS       DASTMT010000088         6821 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_3633671, whole genome shotgun sequence.
ACCESSION   DASTMT010000088 DASTMT010000000
VERSION     DASTMT010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 6821)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6821)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6821
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3633671"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1411
                     /locus_tag="VFR00_01830"
     CDS             <1..1411
                     /locus_tag="VFR00_01830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0158017.1"
                     /translation="LLSIQSAMLLALGFLVASLLGLLLASAFWSRAVRLTTARIKQSL
                     PVSEQEIQADRDRLRAEYAVKVHKLETNLEQAKLTRARQLVELNRRDANISTLETNLG
                     SLKAELEEHQNARHVLEQTVADRLPKVEARLAEAKRLILSREREIAELLENAKRHKLA
                     LEEAASINAQQTAQIERLTSSVAQRAARKPAGDSAGEAESALRSELEALRAKARDQAN
                     VIDRLQRNASGAQNLASVGKAGEEAARPPQEVLAQAESALAAARAAASGSDQSAALAR
                     QLSALKSKAEDQTGEITRLKAALASFEADDKTQEGKESRAALRARLGAAQAQAEHQSA
                     MVGRLRAELAAVNERLARQAAQFMEEMRRLGAGSMPVAGQPRASSARAERRPLAERVA
                     HVRQAAPPAAASPAKATEPQAPAKVEDAAPPARNGSGGDGESPPSSSAAAEMPKAGDI
                     PGEGRRLRLLDRITSLAKE"
     gene            1534..2004
                     /locus_tag="VFR00_01835"
     CDS             1534..2004
                     /locus_tag="VFR00_01835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0158018.1"
                     /translation="MSSLPALAIDNTAPPLTPPPRSMRLSDAEKIAADRLDSTSISSL
                     KAALAGVTPEEAQAGAPRLVERIDAATRLATDATAAAEAVERLKRVLQAQLVASRPSV
                     PAEVAMTQPLTDAAKPRTTPPLPEQPRPDIRGFLAGFVLSGAIGAVLYLYMMAG"
     gene            complement(1986..3659)
                     /locus_tag="VFR00_01840"
     CDS             complement(1986..3659)
                     /locus_tag="VFR00_01840"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HEU0158019.1"
                     /translation="MPRLARSQSHLRARYDAVVVGSGYGGGVAAARLARAGKRVAVLE
                     RGREIAVGEFPRRFSDLRSDLQLSGRQVRLGSPTGLYDVRLGADMHVLVACGLGGGSL
                     INAGVALRPDPRVFEDPAWPRELAQDPLLDAGFHAAARWLRPARDPAAHERGKMKALR
                     AASVALGVDPVAAPVTVSFEPNVNAAGIAQPACTLCGDCCGGCNVGAKNTVALTYLPD
                     AVAHGAEIFTHARVRHLSPDARGGWQVHFAVQPGAEAAEAEVVRSISAKLVVLAAGSL
                     GSTEILLRSRREGLALSDRLGQRFSANGDIIAFGWGGKLPVGAIGIGAPPKVEDMVVG
                     ATVSGQIEIVDDAELANSLTIQEGALPSAMAPILPVLFVPNGRLLGALQSLISGVYKG
                     PFAGLQTFFAVSHDSASGQLLLAADRLELNWPGAREEPVYRRLDAVLEALVRQAQGSY
                     VKNPLAGTLMGQQPATAHPLGGCGIGADRHSGVVNHKGQVFAANAGATAVHAGLYVMD
                     GAIMPRSLGANPLLTITALAERAMRHMARDHGFELGAASAANEAQPAIM"
     gene            4004..5848
                     /locus_tag="VFR00_01845"
     CDS             4004..5848
                     /locus_tag="VFR00_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015684121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU0158020.1"
                     /translation="MQAGDVAQASMGQTAPTNISNPKYFHKVVDCQWACPTHTPVPEY
                     IRLIAQKRYAAAYMLNWESNVFPGILGRVCDRPCEPACRRGRVEEKPVAICRLKRVAA
                     DYKGEIDAFMPEIPAAKNGKRIALIGAGPASLTVARDLMPLGYSCVLFEKDARGGGLM
                     RTNIPSFRLPETVLDEEIDRVLAFGVEARFGSEIKSLQALLGEGFDAIFIGTGAPKGK
                     DLEISGRREAAANIHIGIDWLTSVAFEHVSRIGKRVVVIGGGNTAMDCCRTALRLGGE
                     HVTVTVRSPRRVMKASPWEIEDAEHEGIPIFDNHAPKAFVIEHGKLVGVRFQRMREVG
                     IDTRGRPRLEPSGEEVVFACDDVLMAIGQENSFPWIERNIGLAFDEWGMPKVDKLTMM
                     SSREGVFFGGDAAWGPENIIWAVAHGHQAAVSIDQYCHGRPLGERPASGSSLFSQKMG
                     IHEWSYKNDYAAQRRVGMPHADKLEALRNLKVEVELGFDDQLAFKEAERCLNCDVQTV
                     FSAPLCIECDACMDICPVDCINFTGNADEPALRAKLRVPARNKTQDLYVSNPLKTGRI
                     MAKDEDVCLHCGLCAERCPTGAWDMQKFMYVEAQAAPACLTYHNAYLR"
     gene            5868..>6821
                     /locus_tag="VFR00_01850"
     CDS             5868..>6821
                     /locus_tag="VFR00_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012029370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HEU0158021.1"
                     /translation="MSGINDFVVKFATVNGSGSASANTIFAKTLFRMGLAVSPKNIFP
                     SNIQGLPTWYEVRVSELGYIARRDGIDLMVAMNPQTFKEDLAEIVPGGWLLYDSTLPR
                     VWPREDINVLGIPIAQLCAQKWSDPRQRQLFKNMVYVGALTAFFEMDVEVLQGAVGDQ
                     LKGKEKLVAANMEAVNLGRDFAKQNFACPLPIRVRPAQCSKGKIMVSGNEAAGLGAIY
                     AGATVCAWYPITPSTSLAEAFEKYARRLRVNKTGKRLYGIVQAEDELAAIGVAIGGGW
                     NGARAFTATSGPGISLMSEFLGLAYFAEVPVVLFNIQRGGPS"
BASE COUNT         1247 a         2264 c         2288 g         1022 t
ORIGIN      
        1 cttgctctcc attcaatccg ccatgctcct ggctttgggc ttcctggtgg ccagcctgct
       61 cggactgctg ctggcgtccg ccttttggag ccgggcggtg cgcctgacca cggcccgcat
      121 caagcagtcc ctgcccgtca gcgaacagga aatccaggcg gatcgcgatc ggctgcgggc
      181 cgagtatgcc gtcaaggtgc acaagctcga gaccaacctc gagcaagcca agctcacccg
      241 cgcgcgccag ctcgtcgaac tcaaccgacg cgatgccaac atttcgacgc tggagaccaa
      301 cctcggttcc ctcaaggccg agttggagga gcaccaaaat gcccggcacg tcttggagca
      361 aaccgttgcc gaccgattgc ccaaggtgga ggcgcgtctc gccgaggcca agcgcctgat
      421 cctcagccgc gagcgcgaga tcgccgagct gttagagaat gccaagcgac acaagctggc
      481 gctggaagaa gccgcctcca tcaacgccca gcagaccgca caaatcgagc gcttgacgag
      541 cagcgtcgcc cagcgggctg cgcgcaagcc ggccggcgat agcgccggcg aagcggagag
      601 cgcgctcagg agcgagcttg aggccctgcg cgccaaggcg cgcgatcagg ccaatgtcat
      661 cgatcgcctg cagcgcaacg ccagcggagc gcaaaacctg gcctccgttg gcaaggccgg
      721 cgaggaggcg gcaagaccgc ctcaggaggt gctggcacag gccgaaagtg cgctcgccgc
      781 ggcgcgcgcg gcggcctcgg gcagcgatca gagcgcagcc ctggcgcggc agctcagcgc
      841 gctcaaaagc aaagccgagg accagaccgg cgagattacc cggctcaagg cggcgctggc
      901 gagcttcgag gccgacgata agacgcagga gggcaaggag agccgcgccg cgctgcgggc
      961 gaggcttggc gccgcccagg cgcaggccga gcaccagtcg gcgatggtcg gcaggttacg
     1021 ggcggagctg gcggccgtga acgagcggct cgcccgacag gccgcccagt tcatggagga
     1081 gatgcgccgt ctcggggcgg gcagtatgcc ggtcgccggg cagccgcgag cctcaagcgc
     1141 ccgcgccgag cgccggcccc tggcggagcg cgtggcccac gtgcggcagg ccgcgccgcc
     1201 ggcggcggcc agtcccgcca aagcgaccga gccgcaggcg cctgccaagg tcgaggacgc
     1261 ggcgccgccc gccaggaatg gcagcggcgg cgacggcgaa agcccgcctt cctcgagcgc
     1321 tgccgcagaa atgcccaaag ccggggacat acctggtgaa ggtcgccggc tgcgattgct
     1381 ggatcgcatc acgagcctgg ccaaagagta gccgggtcgt ggggctgagc ggaccgctgt
     1441 ttttggcggg ccagctgagc tcagcgagga aggcaaggac cggcaggcgg aggcggggcg
     1501 aagcaagcag agtacgagtg tcgaggggtt cagatgtcga gtttacctgc gttggcgatt
     1561 gacaacaccg cacccccgct cactccgccg ccccgctcga tgcggctcag cgatgccgaa
     1621 aagatcgccg ccgatcgcct cgattccaca agcatcagct cactgaaggc cgccctggcc
     1681 ggcgtcaccc ctgaggaggc ccaagctggc gcaccgcgcc tggtcgagcg catcgacgcc
     1741 gccacacgat tggcgaccga cgcaacggcg gcggcggaag ccgtggaaag gctcaagcgc
     1801 gtcttgcagg ctcagctggt ggcgagccgc ccgtccgtgc cggccgaggt tgcgatgaca
     1861 cagccgctga ccgacgccgc caagccgcgc accacgccgc cgcttcccga gcagcctcgc
     1921 ccggatatcc gaggcttcct tgcgggcttc gtcctctcgg gggcgattgg cgcggtgctc
     1981 tacctctaca tgatggcggg ctgagcctcg ttcgcggccg aagcggcccc gagctcgaag
     2041 ccatggtcgc gggccatgtg ccgcatggcg cgctcggcca gcgcggtgat cgtcagcaga
     2101 ggattggcgc ccagcgaacg cggcatgatc gcgccgtcca tgacgtagag accggcatgg
     2161 acggccgttg cacccgcatt cgcggcaaac acctgcccct tgtggttgac gacgccgctg
     2221 tggcgatcgg caccgatgcc gcagccgccg agcgggtggg cggtggccgg ctgctgcccc
     2281 atcagggtgc ccgccagcgg gttcttcacg tagctgccct gcgcctgtcg caccagcgcc
     2341 tcaagcaccg cgtcaagccg ccgataaacg ggttcctcgc gggcacccgg ccagttgagc
     2401 tcgagccggt cggcagccag gagaagttga cccgaggcgc tgtcgtgaga gacggcaaaa
     2461 aaggtctgca gcccggcgaa ggggccctta tagacgccgc tgatcaggct ttgcagggcg
     2521 ccgagcaagc gcccgttcgg cacgaacaga acgggtagaa tcggcgccat tgccgagggc
     2581 agggcgccct cctggatggt caggctgttt gcgagctcgg cgtcgtcgac aatctcgatc
     2641 tgcccggaaa ccgtcgcccc caccaccata tcctcgacct tgggcggcgc gccgatgccg
     2701 atggcaccga ccgggagctt gccgccccag ccaaaggcga tgatgtcgcc gttggcggaa
     2761 aagcgttgac cgagtcgatc ggagagcgcg aggccctctc ggcgcgagcg cagcagaatt
     2821 tccgtggagc ccaagctacc cgcagccaac acaacgagtt tcgccgaaat gctgcgcacg
     2881 acctccgcct ccgccgcctc ggcgccgggc tggacggcga agtggacctg ccagcccccg
     2941 cgcgcatcgg gcgagagatg gcgaaccctg gcatgcgtga agatttccgc gccgtgcgcg
     3001 accgcgtccg gcagataggt gagcgccacc gtattctttg ccccgacgtt gcagccaccg
     3061 caacaatccc cgcacagcgt acaggccggt tgggcgatgc cggccgcatt gacgttgggc
     3121 tcgaaactca ccgtcacggg cgcagcaacc gggtcgacgc cgagcgcgac actggccgcc
     3181 cgcagcgcct tcatcttgcc gcgctcgtgc gcggcgggat cgcgcgccgg ccgcagccaa
     3241 cgcgccgctg catggaagcc cgcatccaac aacgggtcct gcgcaagctc ccggggccaa
     3301 gctggatcct caaacacgcg cgggtcgggg cggagcgcga cgcccgcgtt gatcaacgat
     3361 cccccaccca ggccgcaggc cacgaggacg tgcatgtcag ccccaaggcg cacgtcataa
     3421 agccccgtgg gcgagcccag gcgcacctgc cggcccgaaa gctgcagatc gctacgcagg
     3481 tcggagaatc ggcgcggaaa ctcccccacc gcgatctcgc gaccgcgctc cagcacggcg
     3541 acccgcttgc cggcgcgcgc gagccgggcg gcggcgacgc cgccgccata gcccgacccg
     3601 accacgacgg cgtcgtagcg ggctcgcagg tggctttggg aacgggcaag tcgaggcatg
     3661 aacatcccgg caagatcaaa cgagcgcgac gcctatgccg gtgcgctctg cgccaggcaa
     3721 gggccgaggc gccggacaag cggggttacc aacagcttcg ggcggcgcgc gccccgcggc
     3781 aaagcgagaa cgcagccgtg cgtcgcgtgt tcccgcaatc ccctcggcgc gcctcttttg
     3841 cactgcacaa tgaccaaagc caatgggcat gctagatgcg ccaccgcgcg cgttcgccgg
     3901 ttgaaaaatc tggtttggtc ctatcattgc tgttgggaca agaagacgcg atcttgtaga
     3961 agaagcggtt ggggcgaggc gaagtcaggg gcaggaacgc aaaatgcagg cgggcgacgt
     4021 cgctcaggca agcatgggcc aaacggcccc aactaacatc tccaatccga agtacttcca
     4081 caaggtcgtt gattgccagt gggcctgtcc gacgcacacc ccggtgcccg agtacattcg
     4141 cctgatcgcg caaaaacgct acgcggcggc ctacatgctg aattgggaga gcaacgtctt
     4201 tcccggcatt ctcggccgcg tctgcgaccg accgtgcgag cccgcctgcc ggcgcgggcg
     4261 cgttgaggaa aagcccgtcg ccatctgccg cctgaagcgc gttgccgccg actacaaggg
     4321 cgagatcgat gccttcatgc cggaaattcc ggcggcgaaa aacggcaagc gcatcgccct
     4381 gatcggcgcc gggccggcct ccttgacggt ggcgcgcgat ctgatgccgc tgggctacag
     4441 ctgtgtacta tttgaaaagg acgcgcgcgg cggcggcttg atgcgcacga atatcccctc
     4501 tttccgcctg cccgagacgg tgctcgacga ggagatcgat cgcgtcttgg ccttcggggt
     4561 ggaggcccgc ttcggcagcg agatcaagag ccttcaggcc ttgctgggcg aaggtttcga
     4621 cgcaatcttc atcggcaccg gcgcccccaa aggcaaggac ctcgagattt ctgggcgacg
     4681 cgaagcggcc gccaacatcc acatcggcat cgactggctc acctccgtcg ccttcgaaca
     4741 cgtgagccgc atcggcaagc gcgtggtggt gatcgggggc ggcaataccg ccatggattg
     4801 ctgccgcacg gcgctgcggc ttgggggcga gcacgtcacg gtcaccgtgc gctcaccgcg
     4861 ccgcgtgatg aaggcctccc cgtgggagat cgaggatgcc gagcacgaag gcattccgat
     4921 tttcgacaat cacgcaccca aggcgtttgt gatcgagcac ggcaagctcg tcggcgtccg
     4981 cttccagcgc atgcgcgagg tcggtatcga tacgcgcggc cggccgcggc tcgagccctc
     5041 cggtgaagag gtagtgtttg cctgcgatga cgtgctgatg gcgatcggcc aagagaacag
     5101 cttcccttgg attgagcgca acatcggtct cgccttcgac gaatggggca tgccgaaggt
     5161 cgacaagctc accatgatgt cgagccgcga gggcgtcttc ttcggtggcg acgcggcgtg
     5221 ggggccggaa aatatcattt gggcggtcgc gcacgggcac caggccgccg tgtccatcga
     5281 ccaatactgt cacggcaggc cgctcggcga acgtcccgcc tccggcagca gtctcttcag
     5341 ccagaagatg ggcattcacg agtggtccta taaaaacgat tacgccgcgc agcggcgcgt
     5401 aggcatgccg cacgccgaca agctcgaggc gctgcgcaac ttgaaagtgg aagtcgagct
     5461 gggttttgac gaccagctcg cgttcaaaga agccgagcgg tgtctcaact gtgacgtgca
     5521 gacggttttc tcggcgccct tgtgcatcga gtgcgacgcc tgcatggaca tctgcccggt
     5581 cgattgcatc aactttaccg gcaacgccga cgagccggcg ctgcgcgcca aactgcgggt
     5641 gccggcccgc aacaagacgc aggatcttta tgtttcgaac ccgctgaaga ccgggcgcat
     5701 catggccaag gacgaggacg tttgcctgca ctgcgggtta tgtgcggagc gttgtccgac
     5761 cggggcctgg gacatgcaga aattcatgta cgtcgaggcg caggcggcac cggcttgtct
     5821 aacctaccac aatgcctatc tgcgctgatg gagcgatcga tctcaagatg agcggcatca
     5881 acgactttgt ggtgaagttt gccaccgtca acggatcggg gtcggcgagc gccaatacga
     5941 tcttcgccaa gaccctgttc cgcatgggtt tggcggtgag cccgaaaaac atctttccct
     6001 ccaacatcca ggggctgccg acctggtatg aggtgcgcgt ttccgagctg ggctacatcg
     6061 cccgccggga cggcatcgac ctgatggtcg cgatgaaccc gcagaccttc aaagaggacc
     6121 tggcggagat cgtgcccggc ggctggctgc tctatgactc gacgctgccg cgggtgtggc
     6181 cgcgcgagga catcaacgtg ctcggcatcc ccatcgccca gctctgcgcg caaaagtgga
     6241 gtgacccgcg ccagcgccag cttttcaaga acatggtcta cgtcggcgcg ctgaccgcgt
     6301 ttttcgagat ggatgtggag gtgttgcagg gcgccgtcgg cgatcagctc aagggcaaag
     6361 aaaagttggt ggcggccaac atggaggcgg tcaacctggg ccgcgacttc gccaagcaga
     6421 actttgcctg cccgctgccc attcgggtgc gcccggcgca atgctcgaag ggcaaaatca
     6481 tggtttcggg gaacgaggcg gccggcctgg gggccatcta tgccggcgcc acggtgtgcg
     6541 cctggtatcc gatcacgcca tcaacctcgc tggcggaggc attcgagaaa tatgctcgcc
     6601 gcctgcgcgt caacaagacg ggcaagcgcc tttacggcat cgtgcaggcc gaggatgagt
     6661 tggccgccat cggcgtcgcc atcggcggcg gctggaacgg cgcccgcgcc ttcaccgcca
     6721 cgtcggggcc cggcatctcc ctgatgagcg aatttttagg gcttgcctac ttcgccgaag
     6781 tgccggtggt gctgttcaac atccagcgcg gcggaccctc c
//
LOCUS       DASTMT010000089         1987 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_2674291, whole genome shotgun sequence.
ACCESSION   DASTMT010000089 DASTMT010000000
VERSION     DASTMT010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 1987)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1987)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1987
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2674291"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            623..1774
                     /locus_tag="VFR00_01855"
     CDS             623..1774
                     /locus_tag="VFR00_01855"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEU0158022.1"
                     /translation="MPKIALTDAAVKKLKPPATGQLDIFDKHYPGLVLRLGARTRTWY
                     CMVRVGKRLVRHRLACYPDKSLGEARELWRKTRETVDAGKPPRAAPLNRDAVESVVAE
                     WLKRDQADNATYAEVKAAFHALVIPEWSGRLVTSIDRRDVLDILDAIADRGKIGRANK
                     LHAWLSRFFTWSVGRGVIAQSPMAKLPKHGAPVVRDRALSDPEIAALWRACKALGWPY
                     GSAVQILLLTLARREEIVGLRREEIDAAAALIRLPAKRVKNDQARIIPLGPLAWRILQ
                     DGPRIAGSPFVFTSGTGSTPVSGWSKAKAALDRAMGVNEPWRLHDLRRTGATGLERMG
                     VPLPVTEAVLGHTSGSKAGIVRVYQQHDYEAEKRAALGKWAGHVLALVG"
BASE COUNT          325 a          631 c          682 g          349 t
ORIGIN      
        1 gcgtgcgcgg ggagatgatg gacgatccgg tggccttcgc catcaaggac aagacctcga
       61 tcgggctgct gtgcctgctg ctggtctgct tcggggtggc ttggctcggg taaccggaga
      121 ccgggtgggg gaatgaggat cgtcgcggcg ctcgccctgg cagggcttgc cctcatcgca
      181 gcgcccagtc cgggtgcggc gcgcatccac ggccagatgt actgctgggc gctcgactcc
      241 gagctccccg tcgtgtgtga cgccgaggag gcggaagagg aggttttagg ggtcagattt
      301 caggaattag ttaggttggt ggagcgccaa gctgcccagg aacggcgcgg ccgcgcaatc
      361 cgacccatgc cgtcattttc atctattgca gtttttgttg gtgccgcagg agggatttga
      421 acccccgacc cacgcattac gaatgcgaaa ttggcccgtt tcatcccgtt gcgcaccgtt
      481 gcatgaaacc caccagaagc cgcaagacat gccgataaat agcgggtttc tgcgctatat
      541 tccgttgcac cgcgttgcaa gctatatcat acttggtttg ttacctagta cctagacgct
      601 gttacctacc tgggccacgc atatgccgaa aattgctctc accgacgctg ccgtcaagaa
      661 gctgaagccg cccgccacgg gacagctcga catcttcgac aagcactatc ccggcctcgt
      721 gctgcgcctg ggggcgcgca cgcggacttg gtactgcatg gtgcgcgtcg gcaagcgtct
      781 cgtgcgccac cgcctcgcct gctacccgga caagagcctc ggcgaggcgc gggagctgtg
      841 gcgcaagaca cgggagacgg tcgacgccgg caagccgccg agggcggcgc cgctcaaccg
      901 ggatgccgtc gagagcgtgg tggcggagtg gcttaagcgc gaccaggcgg acaatgccac
      961 ctacgccgag gtgaaggccg cgtttcatgc gctcgtcatt cctgagtgga gcggccggct
     1021 ggtcacctcg attgaccgcc gcgacgtgct cgacattctc gacgccatcg ccgaccgcgg
     1081 gaagatcggc cgcgccaaca agctccatgc atggctcagc cggttcttca cgtggtcggt
     1141 tggccgcggg gtcatcgctc aaagcccgat ggcgaagctg ccgaagcacg gggcgcccgt
     1201 ggtgcgcgat cgggcactgt ccgacccgga gatcgccgcg ctgtggcggg cctgcaaggc
     1261 gctcggctgg ccctacggct cggctgtgca gatcctcttg ctgacgctgg cgcggcgcga
     1321 ggagatcgtg ggcttgcggc gcgaggagat cgacgccgcc gccgcgctga tccggctgcc
     1381 ggccaagcgg gtgaagaacg accaggcgcg catcatcccg ctcggcccgc tggcgtggcg
     1441 catcctgcag gacggcccgc gcatcgccgg cagtcccttc gtgttcacgt cgggcaccgg
     1501 ctcgacgccc gtctcgggct ggagcaaggc caaggccgcc cttgaccggg cgatgggcgt
     1561 caatgagccg tggcggctgc atgacctgcg ccgcacgggc gcgaccgggc tggagcgcat
     1621 gggcgtgccg ctgccggtga cggaggccgt gctggggcac acgtcgggct caaaggccgg
     1681 catcgtgcgc gtctatcagc agcacgacta cgaggccgag aagcgcgcgg cgctgggcaa
     1741 gtgggccggg cacgtgctgg cgttggttgg gtgaactgcg agtaatcgct tcaagcctat
     1801 tgcatgaact tgactttggg cattgtttgt acagcaccgc gattcagaga ggtgctgcgt
     1861 atgtccctac ccgcgaccca atccgccgag gcggcctatc tcacgtcggc catggtgcgg
     1921 cgccgctacg gcggcaagag cgacgcctgg cttttccgtc tcctgcagac cgaccagaca
     1981 ttcccgc
//
LOCUS       DASTMT010000090         3290 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_50380, whole genome shotgun sequence.
ACCESSION   DASTMT010000090 DASTMT010000000
VERSION     DASTMT010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3290)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3290)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3290
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50380"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1555
                     /locus_tag="VFR00_01860"
     CDS             <1..1555
                     /locus_tag="VFR00_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006019197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU0158023.1"
                     /translation="LQKAQEQLVQAQKMEGIGQLTGGVAHDFNNLLTIIMGNLETLQR
                     GLQAPRLDAARLARSTDNALRGAQRAAALTQRLLAFSRRQPLDPKPVDVSRLVSGMSD
                     LLRRTIGEQIAIEAVLAGGLWRAHVDPNQLEVAILNLAVNARDAMPDGGKLTIETANA
                     YLDEGYAAAQAEVVPGQYVSICVSDTGHGMAKQVLERAFEPFFTTKEVGQGTGLGLSQ
                     VYGFVKQSGGHIKIYSEPGQGTTVKLYLPRLHGEAVIQKEPDLRSLPRSADAETILVV
                     EDDDDVRAYSKEVLRELGYSVLEASTATAALRMLDQHPQIDLLFTDVGLPGGMNGRQL
                     ADEARKRRPNLKILFTTGYARNAIVHEGRLDPGVQLVTKPFTYAAVGAKLRDILDARS
                     RPRSVLFVEDEALVRILATEYLEDAGYRVEAAVSATEAMGKVRLLHGEIDAAIIDVGL
                     PDRRGDVLVSELRTLYPALPIVIASGYEEAELRARFKGDSGIAFLNKPYTQDQLVQGL
                     RRACQGGGA"
     gene            1762..2718
                     /locus_tag="VFR00_01865"
     CDS             1762..2718
                     /locus_tag="VFR00_01865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0158024.1"
                     /translation="MVRGPDMRVEAHGTLLRVTSAAGLIYEGSTSAGDGRLEEFFAAY
                     DQAFVLPNEKEGLAGFADCLALNEGCPYDRLSRRYGPFREFIVMARTAGEMVGGLNFI
                     SFPLLDADENRAALSLNLNYIFVVAAQRRRGVLKRMVADLPQLALAMFAHSHAPSPLS
                     DGAAPPSVYCFIEQNDPYRMSAGDYALDTEATGLDQLARIALWARQGARIIDFAYVQP
                     ALTALQLADPNLVYAVLGAPRPTLHPDFLRQHLERFFAISVLKGGDPRANAEARQQLD
                     SLAACAAVGGRLALLEVPEISRLPAPGGAGARPTLRTVLGPV"
     gene            2833..>3290
                     /locus_tag="VFR00_01870"
     CDS             2833..>3290
                     /locus_tag="VFR00_01870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0158025.1"
                     /translation="MHTLDWLWQTIKPHKEELSLLIGALTLSYVLYGIARAGNFISEV
                     AFEPQEGWFPRSVQWIRGVARALLLSWYAPTLLFAFPRRRFVGARYYVELQILQPRVS
                     LPSRRDYTAPEYARRLEKAQVAEQRRRERVRQLLRERLTEEPGVLVAVLG"
BASE COUNT          556 a         1077 c         1064 g          593 t
ORIGIN      
        1 cctacaaaag gcgcaagaac aactcgtaca agcgcagaaa atggagggta tcggccagct
       61 gaccggcggc gtggcacacg atttcaataa cttactgacg atcattatgg gcaatctcga
      121 gacgctgcag cgcggcctgc aggcgcccag gctcgacgcc gctcgactgg cgcgctcgac
      181 cgacaatgcg ctgcgcggcg cgcaacgggc tgccgcgctc acgcagcggc tgctcgcctt
      241 ctcgcgccgg cagccgctcg atccgaagcc cgtcgacgtc agccgtcttg tcagtggcat
      301 gtcggatttg ctgcgacgta cgatcggcga gcaaattgcc atcgaggctg tgctcgccgg
      361 cggtctctgg cgggcccacg tcgatcccaa ccagctcgag gttgccatcc tcaacctggc
      421 ggtcaatgcg cgcgacgcca tgcccgatgg cggcaagctg acgatcgaga ccgccaatgc
      481 ttacctcgat gagggctatg ccgccgccca ggcggaggtg gtgcccggcc agtacgtctc
      541 aatctgcgtc agcgacaccg gccatggtat ggccaagcag gtgctcgagc gcgcttttga
      601 gcccttcttc acgaccaaag aagtcggaca aggtaccggc ctcgggctga gccaagtcta
      661 tggcttcgtc aagcaatcgg gcggccacat caagatctac tccgagccgg gacagggcac
      721 caccgtcaag ctctacttgc cgcgcctaca cggcgaggcc gtcattcaaa aggaaccgga
      781 tctgcgtagc ttgccgcgca gcgccgatgc cgagaccatt ctggtggtgg aagacgacga
      841 cgacgtgcgc gcctattcca aagaggtcct acgcgagttg ggctactcgg ttctcgaggc
      901 ctcgaccgcc accgccgcct tgcgcatgct cgatcaacat ccacaaatcg atctgctgtt
      961 cacggatgtt ggtcttcccg gcgggatgaa cggccgacaa ctcgcggacg aggcacgcaa
     1021 gcgccgtccc aacctcaaaa tcctttttac gacgggctat gcgcgcaacg ccatcgtgca
     1081 cgagggccgg cttgatcccg gcgtgcagct cgtcaccaag ccattcacgt acgccgccgt
     1141 cggggccaag cttcgcgaca ttctcgatgc gcgctcccgg cccagaagcg tcctctttgt
     1201 cgaggacgag gccctggtgc ggatccttgc caccgaatat ctcgaggacg cgggctatcg
     1261 ggtcgaggcg gccgtgtccg ccacggaggc gatgggcaag gtccgcctgc tacatggcga
     1321 aatcgacgcc gccatcatcg atgttggtct acccgatcgc cgcggtgacg tcctcgtcag
     1381 cgagctgcgt acgctctatc cggcattgcc gatcgtcatc gccagcggct atgaggaggc
     1441 ggaattgcgc gcgcgcttta agggcgatag cggcatcgcc tttctcaaca agccctacac
     1501 gcaggaccag ctggtgcaag gcttgagacg ggcgtgccag ggcgggggcg cctgagccca
     1561 aagagccgct ggtccaggcc gttgccttgc cgcgcgagcc gccagcggcc tgttacgacc
     1621 tggccccggc aagcggatcc ttgccggcca aggtgcaaaa aatttgctat gctgcggacc
     1681 ggcttggcgg acattggcgt gggtcaaggc cgccgggcac atggcaaagt ccttgcggca
     1741 ggggtgcatc agaaggggtc catggttcgc gggcctgaca tgcgggtcga ggcgcacggc
     1801 accttgctgc gggtgacgag cgcggcgggt ctcatctacg agggctcgac ctcggctggg
     1861 gacggacggc tcgaggaatt ttttgccgct tacgatcagg ccttcgtgct gccgaatgaa
     1921 aaggagggcc tggcgggctt tgccgactgc ctcgccctca acgagggttg cccgtatgac
     1981 cggctcagcc gtcgttacgg tccttttcgc gagtttatcg tcatggcccg cacggccggc
     2041 gagatggttg ggggactgaa cttcatcagc tttcccctgt tggatgccga tgagaaccgg
     2101 gcggcgctca gcctgaattt gaactatatc ttcgtcgttg cagcccagcg tcgtcgcggt
     2161 gtgctcaagc gaatggtcgc cgacctaccg cagctggcgc ttgccatgtt cgcgcacagc
     2221 cacgcacctt cccctctgag cgacggcgcc gcgccgccga gcgtctattg ttttatcgag
     2281 cagaacgatc cctatcgcat gagcgcgggt gactacgcgc ttgatacgga ggccaccggg
     2341 ctcgatcagt tggcgcggat tgcgctgtgg gcgcgccagg gcgcccgcat catcgacttt
     2401 gcctatgtgc aaccggcgct caccgcgctg cagttagccg atcccaacct cgtctatgcg
     2461 gtcctgggcg cacccagacc gacgctgcat ccagactttc tgcgccagca cctcgagcgc
     2521 ttttttgcca tctcggtact caagggaggt gacccccgcg ccaacgccga ggcgcgccag
     2581 cagctggaca gccttgccgc atgcgcggcg gtgggtggcc ggctggcgct gctcgaagtt
     2641 ccagagatca gccgccttcc cgcgcctggc ggcgccggcg cgcgcccgac gctgcggacg
     2701 gtgcttgggc ctgtgtaaca attcaatcac aggcgcgctt ggatccgccg cccgccgcag
     2761 gcgtcggcta caggccccga tccgggcccg gtaatctgac cgggtcgggc aggcggggtt
     2821 ggcggggacg caatgcacac gctggactgg ctgtggcaga ccatcaagcc gcacaaggaa
     2881 gaactctcgc tcctgatcgg ggcgctgacg ctcagctacg ttctctacgg catcgcccgc
     2941 gcgggcaact tcatcagcga ggtggcgttc gaaccgcagg agggctggtt tccgcgctcg
     3001 gtacagtgga tccgcggcgt tgcccgcgcc ttgctcctat cctggtatgc gcccacgctg
     3061 ctgtttgcct ttccgcgccg gcgtttcgtc ggtgcgcgct attacgtcga attgcagatc
     3121 ctgcagccgc gggtttcgct gccgagccgg cgtgactaca cggcgcccga atacgcccgg
     3181 cgcctggaga aagcgcaagt cgccgagcag cgtcgccgtg agcgggtacg ccagttgttg
     3241 cgcgagcgcc taaccgagga gccgggcgtg ctcgtcgccg tgctggggtg
//
LOCUS       DASTMT010000091         2240 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_379848, whole genome shotgun sequence.
ACCESSION   DASTMT010000091 DASTMT010000000
VERSION     DASTMT010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2240)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2240)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2240
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_379848"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            175..1539
                     /locus_tag="VFR00_01875"
     CDS             175..1539
                     /locus_tag="VFR00_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013419803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEU0158026.1"
                     /translation="MSTRPSVRLAAIPFTHSVAGCLSSAIGEHGLTAHELNFWLDKLV
                     APLAELKRGYRDRTLALLRIAEDTADLEAAERAYGRLVAGARAIVFFGIGGSSLGGQA
                     LAQLGGWSIPGTADAAQKARPRTRFYDNLDAHTLGAALGAFDLGSARFVVTSKSGGTP
                     ETLVQALAALNAVKQAGLEHRIPELFLGITEPAREGKANGLRRLFEAHGIALLDHHTA
                     IGGRFSALTNVGLLPALARGLDVRALRAGARSVVEALLSASAPPDCTPAVGAAVSVAL
                     ARERAVRTQVMLPYSDRLSRFAHWFVQLWAESLGKNGQGTTPLACLGPVDQHSQLQLF
                     MDGPREHLITVVRAPTAGEGPRLSTELANMAGLDYLGGKAVGDLVAAQAVAVPEALAR
                     AGRPVRVFELERLDEASLGALMMHLMLETILAGRLLGVDPFDQPAVELAKVITRERVA
                     ALRA"
     gene            1576..>2240
                     /gene="mutL"
                     /locus_tag="VFR00_01880"
     CDS             1576..>2240
                     /gene="mutL"
                     /locus_tag="VFR00_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEU0158027.1"
                     /translation="MPIRQLSAETINRIAAGEVIERPASVVKELVENAIDAGAKSIEI
                     VTVAGGLSLIRVSDDGIGMSAADLGLAVERHATSKLAEEDLLNIATLGFRGEALPSIG
                     AIAHLTVAARARHESQAHEIVVDRGAKRAVRPVALGGGSRVEVRELFSATPARLKFLK
                     SERAENAAIAEVVKRIALAHPCIAFSLTTGERTGLRLPHEPAGADGHLARLGRIMGRD
                     FLA"
BASE COUNT          348 a          749 c          777 g          366 t
ORIGIN      
        1 gccgccgaac tgctggccgc gctcgagcag cgcatggaag ttaacctcaa gaaaccgggt
       61 accgtcgagt tcgctcaggt tttcgatgag ctggcacgac gttaggcggc ggtgctgccg
      121 gtcgcagcgg cgccacgctt ccctcgcaca acaaatcgcg ctaggcctcg cgccatgagc
      181 acacgcccaa gcgtccgcct tgccgccata cccttcacgc actcggttgc gggttgcctg
      241 tcgagcgcca tcggcgagca cgggctgacc gcgcacgagc tcaacttctg gctggacaag
      301 ctcgtggcgc cgttggccga gctcaagcgg ggctaccgcg accgtacgct ggcgctgttg
      361 cgcattgccg aggacacggc ggacctggag gccgcggagc gggcctacgg ccggctggta
      421 gcaggggccc gcgccatcgt ctttttcggc atcggcggct cgagcctcgg tggccaggcg
      481 ttggcccagc ttggcggctg gagcattccc ggtactgccg atgcggcgca gaaggcccgc
      541 ccgcgaacgc gcttttacga caacctcgac gcgcataccc tgggcgccgc gctcggcgcc
      601 tttgacctcg gcagcgctcg ctttgtcgtg acctcaaaat ccgggggcac gccggagaca
      661 ctggtgcagg cgctggcggc gctcaatgcc gtaaagcagg cgggtctcga gcaccgcata
      721 cccgaattgt ttctgggcat taccgagccc gcgcgcgaag gcaaggccaa cgggctgcgc
      781 cggctgtttg aggcccacgg cattgcactg ctcgatcatc acaccgctat cggcggccgc
      841 ttttccgccc tgaccaacgt cggtctgcta ccggcgctgg cgcgcgggct cgacgttcgc
      901 gccttgcggg ccggcgcgcg cagcgtggtg gaagcgcttt tgtcggcgtc ggcgccgccg
      961 gactgtacgc cggccgtagg tgccgctgta tcggtggcgc tggcgcgaga acgtgccgtg
     1021 cgcacgcagg tgatgctccc ctattcggat cggctcagcc gctttgcgca ttggttcgtg
     1081 cagctgtggg cggaaagcct gggcaagaat ggccaaggta ccacgccgct cgcctgcctg
     1141 ggtcccgtcg atcagcacag ccagctgcag cttttcatgg atgggccgcg cgagcatctg
     1201 atcacggtcg tgcgcgcgcc gacggctggc gaaggaccac gattgtccac agaactggca
     1261 aacatggccg ggctcgacta cctcggcggc aaggcggtcg gcgatttggt ggccgcccag
     1321 gccgtggccg taccggaagc gctcgcgcgc gccggacggc cggtgcgcgt cttcgagctt
     1381 gaacggcttg acgaggcaag tctgggcgct ttgatgatgc acctgatgtt ggaaacgatt
     1441 ctcgccgggc ggttgctagg cgtcgatccg ttcgatcagc ccgccgtcga gctggccaag
     1501 gtcataacgc gcgaacgggt cgccgcattg cgggcgtaac ggcgcttcgc acatgccgag
     1561 gaggcagcgc caaccgtgcc cattcgccag ctcagcgccg agacgatcaa ccgcatcgcc
     1621 gccggcgagg tgatcgagcg gccggcaagc gtggtcaagg agctggtcga gaacgccatc
     1681 gacgccgggg cgaagagcat cgagatcgtc acggtcgccg gcggactatc gctgatccgc
     1741 gtcagcgacg atggcattgg catgagcgcg gccgatctgg gcttggccgt cgagcggcac
     1801 gccacctcta agctcgccga ggaggatctg ctcaacatcg ccacgctggg tttccgaggc
     1861 gaagccttgc cgtcgatcgg cgccattgcc cacctcaccg tggcagcgcg cgcgcgccac
     1921 gagagccagg cgcacgaaat cgtggtggac cgcggcgcca agcgcgcggt gcgccccgtg
     1981 gcgctgggcg gtggcagccg cgtggaggtg cgcgagctat tctcggccac gcccgccagg
     2041 ttgaagttcc tcaagagcga gcgagcggag aatgccgcca ttgccgaggt cgtcaagcgc
     2101 atcgccctgg cgcacccttg catcgccttc agcctgacga ccggtgagcg caccggctta
     2161 cggctcccgc atgagccagc gggggcggat gggcatctcg cacgcctcgg ccgcattatg
     2221 gggcgcgact ttctggccga
//
LOCUS       DASTMT010000092         2757 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_3331052, whole genome shotgun sequence.
ACCESSION   DASTMT010000092 DASTMT010000000
VERSION     DASTMT010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2757)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2757)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2757
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3331052"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2757
                     /locus_tag="VFR00_01885"
     CDS             <1..>2757
                     /locus_tag="VFR00_01885"
                     /inference="COORDINATES: protein motif:HMM:NF024516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HEU0158028.1"
                     /translation="TLPHLAALEGFDLPVWGEAYLDLSSDGDILGGRIGIDAAPGTVA
                     LPWLADTAFAIDGGHLEISYQSGARRFEIAPSVLVWGDSRVQFAGSVAHDAGAPEGPA
                     WAFSLKSEGGWIGAELPAHERLPIDDWSAHGFLAPERGRVLLNQFSLRAGGAEVTAAG
                     DVAGMGSQPSARLEGKLGAMPEKTFKALWPAALAPQSRAWIGQRLVRGQLQGGSFKLT
                     SGEEPQGGERASRQSAADRVSLTLVASDLALALNDRWPALELPRALARLDGHTFEISV
                     PEGAFVAGDGRRIGVKGNFSVDMQEPLPRTGHITAKAQGPLSLALEMLAQEPFNAFEQ
                     SGLNPATVEGKLEAQLSLALPLQHAVAPADLKIDGKARISDGRIPQVFPPYDIHAANL
                     VLELTGTAVEARGDMLIKGVPIKANWQHVFGVPQDKQPPLTFTANLDANDRSQLGLDI
                     NDIVLGEVAAEITVTRDAQNEQRVHLRADLANAEIVLDSIAWKKPKGRSSTFQCDIVR
                     NGATHPVELMNVQLVGDNVAVEGWMGVGADHRVREYRFPNFSINVITSLQAHGKMRAD
                     GVWEVTAKGPRYDGKDLFQSFFDVAGLHDRDSKVRPGVDLRAEIDTVVGYNDGALRNV
                     KVWLQKRNNKLTALDVRGVLEGNKAFAAVLRPEPGQARLLRAEANDAGQLFKLVGFYN
                     SAVGGSMNLEVNLEGSGVAQRTGTLWAKNFVLLGDAVSGDVLVQQPDGAVRPIGSADK
                     RGIVREQFSFETLRVPFSVGHGQFLLNEARLEGPVLSANMRGKIDFRSQNVNVGGTFT
                     PLAGLNTMFRGLPLFGPLLTGPRGDGVFAMTFAIQGSLSAPQVIVNPLSVITPGVTRE
                     IMQMAPETQRIVPRDRPQSRGEAAPRPSSAPAAANGSNGFEARVQPEIGGGWSADGEP
                     ATPAK"
BASE COUNT          456 a          908 c          958 g          435 t
ORIGIN      
        1 accttgccgc atctggccgc gcttgagggc ttcgatttgc cggtgtgggg cgaggcctac
       61 ctcgaccttt ccagcgatgg cgacatctta gggggccgga tcggcatcga cgcggcgccg
      121 ggaacggtgg cgctgccctg gctggcggac acggcctttg ccatcgacgg cgggcatttg
      181 gaaatctcct accagagcgg cgcgcgtcgc ttcgagatcg caccctcggt gctggtctgg
      241 ggcgacagcc gcgtgcaatt tgccggcagc gttgcccacg acgcgggcgc cccggaagga
      301 cccgcctggg ccttttcgct caaatccgag ggcggctgga tcggagcgga gctgcccgcg
      361 cacgagcgcc tgccgatcga cgattggtcg gcgcacggct ttctggcacc cgagcgcggc
      421 cgcgtcctgc tcaatcaatt ctcgctgcgg gccggcgggg cggaagtgac ggcggcaggc
      481 gacgttgccg gcatgggcag ccagccaagc gcgcgcctgg aaggcaagct cggcgccatg
      541 ccggaaaaga ccttcaaggc gctctggccc gcggcgctgg cgccgcagag ccgcgcctgg
      601 atcggccagc gcctggtgcg gggccagctg cagggcggct ccttcaagct gacctcgggc
      661 gaagaacccc agggcgggga gcgcgcatcg cgccagagcg cggcggaccg ggtctcgctc
      721 acgcttgtgg ccagcgatct ggcgctggcc ttgaacgatc gctggccggc gctggagctg
      781 ccgcgcgcgc tggcgcgtct cgacggtcat accttcgaga tttcggtgcc cgagggcgcc
      841 tttgttgccg gtgacggacg ccgcatcggc gtcaagggca atttcagtgt cgacatgcaa
      901 gagcccctgc cgcgcaccgg ccacatcacg gccaaggccc agggtccatt gtccttggcc
      961 ctcgaaatgc tggcgcagga acccttcaac gcttttgagc agagcggcct caacccggcc
     1021 accgtcgagg gcaaactgga ggcccagctg tcgctggcgc tgcccctgca gcatgccgtg
     1081 gcgcccgccg acctgaaaat cgacggcaag gcgcgcatca gcgatggccg cataccgcag
     1141 gtgtttccac cctacgacat ccacgctgcc aatctcgtgt tggagctgac gggcacggcg
     1201 gtggaggcgc gcggcgacat gctgatcaag ggtgtgccga tcaaggccaa ctggcagcac
     1261 gtgtttggcg tgccgcagga caagcagccc cccctcacgt tcaccgccaa cctcgatgcc
     1321 aacgatcgca gccagctcgg gctcgacatc aacgatatcg tgctgggcga ggtcgccgcc
     1381 gaaatcacgg tgacgcgcga tgcgcaaaac gagcagcgcg tgcacttgcg cgccgacctg
     1441 gccaacgccg agatcgtgct cgacagcatc gcctggaaga agccgaaggg acgctccagc
     1501 accttccagt gcgatatcgt caggaacggt gcgacgcatc cggtcgagct gatgaacgtg
     1561 cagctggtcg gcgacaacgt ggcggtggag ggttggatgg gcgtgggcgc cgatcaccgc
     1621 gtccgcgagt accggtttcc caatttttcg atcaacgtca tcaccagcct acaggcgcac
     1681 ggcaagatgc gtgccgatgg agtttgggag gtgacggcca aggggccgcg ctatgacggc
     1741 aaggacctgt ttcaatcgtt ttttgacgtg gccggcctgc acgaccgcga cagtaaggtg
     1801 cgcccgggcg tcgacctgcg cgccgagatc gataccgtgg tcggctacaa cgacggggcg
     1861 ctgcgcaacg tcaaagtctg gctgcaaaag cgtaacaaca agttgacggc actcgacgtg
     1921 cgcggcgtcc tggagggcaa caaggccttc gcggccgtgc ttcgtcccga gcccgggcaa
     1981 gcgcggctgc tgcgggccga ggccaacgac gccgggcagc tgttcaagct ggtcgggttt
     2041 tataacagcg cggtcggcgg cagcatgaac ctcgaggtca acctcgaagg ctcgggcgtg
     2101 gcccagcgca ccggcacgct ctgggccaag aatttcgtgc tgttgggcga tgccgtcagc
     2161 ggcgacgtgc tggtgcagca gcccgacggc gccgtgcggc cgatcggatc ggccgataaa
     2221 cgcggcatcg tgcgcgagca gttctccttc gaaacgttgc gcgtgccctt ctccgtgggg
     2281 catggccagt ttctcctcaa cgaggcccgg ttggagggcc cggtgctcag cgccaacatg
     2341 cgcggcaaaa tcgatttccg ttcgcagaac gtcaacgtcg gcggcacctt tacgccgctg
     2401 gcggggctca atacgatgtt tcgcggcctg ccgctgttcg gtccgctgtt gacgggcccg
     2461 cgcggcgatg gcgtgtttgc gatgacgttt gccatccaag gctcgctcag cgcgccgcag
     2521 gtcatcgtca atccattgtc cgtgatcacg cccggggtga cgcgcgagat catgcagatg
     2581 gcgcccgaaa cgcaacgcat cgtgccgcgc gaccggccac agtcgcgcgg cgaagccgcc
     2641 ccgcgcccgt ccagcgcgcc ggcggcggcg aacggcagca acggcttcga ggcgcgggtg
     2701 cagcccgaaa tcggcggcgg ctggtcggcc gacggcgagc cggccacgcc ggccaag
//
LOCUS       DASTMT010000093         9996 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_3635388, whole genome shotgun sequence.
ACCESSION   DASTMT010000093 DASTMT010000000
VERSION     DASTMT010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 9996)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9996)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9996
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3635388"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..963
                     /locus_tag="VFR00_01890"
     CDS             <1..963
                     /locus_tag="VFR00_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT AH/RIS domain-containing protein"
                     /protein_id="HEU0158029.1"
                     /translation="GNACTGAVINGRHLPLTTQLRNGDEVEVLVSKGQTPPAAWEKIA
                     ITGKAKSAIRRAARDALRRQYSELGRHLLITAFERAGYEYSDDRLKKALMRLSQKSVE
                     EVHATVGRGELPVRDVIRAVVPDADLASEPAPRKRNRRERGQGEEGWFNLSKVFNLKF
                     RWPGSGSGEARQTQVLPIRGLRNDVPVTFEEGGAVPGDRIVGVLSEGEGIRIFQIHSP
                     KLKEYENERWIDVTWDVDPERPERFPAKILVTALNEPGTLAQIAQVIGESGGNIDNVR
                     MIRRAKDFTEMLIEVEVWDLDHLTHIMNGVKGKPVVSSVERVFV"
     gene            1120..1920
                     /locus_tag="VFR00_01895"
     CDS             1120..1920
                     /locus_tag="VFR00_01895"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020665367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HEU0158030.1"
                     /translation="MERSTPTPAAHLRLGVNIDHVATIRNARGGRHPDPLRAAHLAVE
                     AGADGITAHLREDRRHISDADIARLKHELTLPLNLEMASTEEMLAIALRHVPHACCLV
                     PEKREERTTEGGLNVEAGENHLRRYVDELKAAGIRVSLFIEPELAAVAASARLGADIV
                     ELHTGAYCERALEDDPAGSKRELDRLKRAAAAAAGAGLEVHAGHGLTFNTVAAVARLP
                     QVVELNIGHFLVGEAIFSGLGPAIRRMRTLMDEARYGIAPRQAAAATS"
     gene            1917..2339
                     /gene="acpS"
                     /locus_tag="VFR00_01900"
     CDS             1917..2339
                     /gene="acpS"
                     /locus_tag="VFR00_01900"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013166443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEU0158031.1"
                     /translation="MILGLGNDIIDIRRIQRTIDRYGERFLGRVFTDTERRKSDARAN
                     RAASYAKRFAAKEACAKALGTGLRGGVFWRDMGVVNLRSGRPTIELTGGAAAQLKRLT
                     PPGFAARIDLTLTDDFPQAQAVVLISGVPVARSENDAG"
     gene            2465..3235
                     /gene="lepB"
                     /locus_tag="VFR00_01905"
     CDS             2465..3235
                     /gene="lepB"
                     /locus_tag="VFR00_01905"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018697681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEU0158032.1"
                     /translation="MSSETEAGKVRESGWRETIKIVVHALILAMIVRIFFYQPFNIPS
                     GSMKQTLLVGDYLFVSKLAYGYSRYSFPWGIVPINGRIFGTEPKRGDVVVFKLPRDNS
                     TDFIKRVIGLPGDEISVRNGVLYINGQAVPKVRKGDFISPEEDRPIPRFEETLPNGVK
                     YYVLDSTPNGQFDNVGPYKVPAGHYFMMGDNRDNSTDSREQSPRFGVGYVPFENLVGR
                     AEIIFFSAAVDDPNAFRLTQPWHWPMDIRWNRIFSLVR"
     gene            3240..3941
                     /gene="rnc"
                     /locus_tag="VFR00_01910"
     CDS             3240..3941
                     /gene="rnc"
                     /locus_tag="VFR00_01910"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013420802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEU0158033.1"
                     /translation="MAPARKLKELETRVGYHFKDRALLRRALTHASVRQASAKRRDNE
                     RLEFLGDRVLGLAVAELLNESYPSASEGELARLYNRLVRGGTCAEIARELNLGTCLVL
                     SESEEGSGGRDKETILADACEALLGAIFLEAGFEKVRDVVRAHWSGRLAKAPAEVADA
                     KSALQEWAQGQGLELPRYVEVERLGPDHAPRFTAEVSIKGKKAARGEGASKRAAEQAA
                     AAALLSREGVWRPAK"
     gene            3938..4849
                     /gene="era"
                     /locus_tag="VFR00_01915"
     CDS             3938..4849
                     /gene="era"
                     /locus_tag="VFR00_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019404172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEU0158034.1"
                     /translation="MSASPASETRCGFIAVIGAPNTGKSTLVNALVGAKVAIVSHKVQ
                     TTRTPLRGIAIEGASQLVFIDTPGLFQPRRRLERAMVEAAWSGAEDADLIALLLDASQ
                     GLDAAAQRILDELAAKTSSLVLVLNKIDRVPKPRLLTLAAEANARLPFAATFMVSALK
                     GSGVADLKRYFAGQVKPGPWHFPEDEISDAPLRLLAAEITREKIYARLHDELPYQTAV
                     ETTAWQERKNGVRIEQTIFVERDSQRSIVLGKGGQMIRQLSMEARQELAQILEQPVHL
                     ILFVKVRQDWENDPERYRQMGLRFPKT"
     gene            4879..5604
                     /gene="recO"
                     /locus_tag="VFR00_01920"
     CDS             4879..5604
                     /gene="recO"
                     /locus_tag="VFR00_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEU0158035.1"
                     /translation="MEWSDEGMILSVRPHGETGAVLELFTRRNGRHLGLVHGGRSRKL
                     RPILQIGNHVEASWKARIADNLGHFALELRKSYAALVLDDAAALAALTSIAALARLLP
                     ERDPHPNLFEVTLFVLGYLDEREVWPALLVRWELALLEELGFGLDLTTCAATGVKTNL
                     SYVSPKSGRAVSAAAGEPYQARLLALPPFLLGPAPEGVTAADVAAGLALTEHFLLARV
                     LRPRDLAMPEARNRLLSYLRPNV"
     gene            5728..6312
                     /locus_tag="VFR00_01925"
     CDS             5728..6312
                     /locus_tag="VFR00_01925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0158036.1"
                     /translation="MPSLKSWRHHPAPTMAALGLVSGILSAVVGFEVRIGVLDWPGTV
                     FFLSAEMMPIGLLFAAVMAFAIAAWHAPAWAPILTFIGTLYAWSAAIQTALFMHRIGG
                     GDASHAGPLLGGVFAGLVGAGLSHLSVALFAPELRDLGQAMRTALVGALAGVLFYFGS
                     RNLIDARILFLVWQPAVAYAIGLGLAARPAPQAS"
     gene            complement(6385..7059)
                     /locus_tag="VFR00_01930"
     CDS             complement(6385..7059)
                     /locus_tag="VFR00_01930"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HEU0158037.1"
                     /translation="MPVLPDLPQTELAIVEMTNAFRKQHKLGTLKPNAALIAAARAFA
                     DYLGRTGKFAHEADGREPHQRAEAQGYRYCLVAENLAFNLDSRGFATSQLARDVVEGW
                     KSSPAHRLNLLEASATEIGVGVARAAGRQPKFISVQLFGRPEALKVSFAIENRTGAAV
                     TYTLGEESETLPARASVNHTSCAAGPLKIDKAGSAARQLEPRDGDTFVLTQGAGGAIK
                     VERGRK"
     gene            7274..9523
                     /gene="parC"
                     /locus_tag="VFR00_01935"
     CDS             7274..9523
                     /gene="parC"
                     /locus_tag="VFR00_01935"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013419052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HEU0158038.1"
                     /translation="MSKTTILAPPPPIEPIKLKDALEERYLAYALSTIMHRALPDVRD
                     GLKPVHRRILYAMRELRLDPAGGYKKCAKIVGEVMGNYHPHGDQAIYDALVRLAQDFA
                     VRYPLVDGQGNFGNVDGDNAAAMRYTESRLTNVAALLLEGIDEDTVDFRPTYDGSNQE
                     PAVLPANFPNLLANGTSGIAVGMATSVPPHNVEELCNALLHLIKHPNAHVEKLVEFVP
                     GPDFPTGGVIVEPRDAMLEAYKTGRGGFRLRARWQREEGGRGTYQIVVTEVPYQVQKA
                     KLVERIAELINDKKLPLLGDVRDESAETVRLVLEPKSRSVDPVLLMESMFRLTELEVR
                     FSLNMNVLSAGQVPGVLSLRDVLRRWLEHRQVVLVRRSKFRLEKIEHRLEVLDGYLIA
                     YLNIDEVIRIVRFEDDPKAKLIKRFKLTEVQADAVLNLRLKSLSKLEEVEIRAEHDKL
                     SKERRGLKSLLKHEDEQWDRIADEIKATRETYAKKTELGRRRSSFADAPSIEIDLEQA
                     MIEKEPITVILSEKGWIRAMKGHLEDVSKLEFKQGDALKRAVKASTTDRLLLLASNGK
                     VFTLAADQLPGGRGHGEPVRLMVDLEENHDFVEIWMHEAGRKLLVGATSGHGFIVPED
                     EMVAMTRKGKQVMNVDSPAEACCVVPVEGDLVAAIGENRKMLIFPLEEVNEMTRGKGV
                     ILQRYKDGSLSDLRVFKKADGLSWLDAAGRTFTLPMAELRDWLGQRAQSGRLAPKGFP
                     RSNKFGPAF"
     gene            9783..>9996
                     /locus_tag="VFR00_01940"
     CDS             9783..>9996
                     /locus_tag="VFR00_01940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0158039.1"
                     /translation="MRAGLVGLIALVLALARAPGAFAETPCNKADFEAVVDEAAAALR
                     SLNHENTPQFQARLRQLKDKRGWSHEQ"
BASE COUNT         1743 a         3353 c         3274 g         1626 t
ORIGIN      
        1 ggcaatgcct gcaccggtgc cgtgatcaac ggccggcacc tgcctttgac gacgcagctc
       61 aggaacggcg atgaggtgga agtgctggtc tcgaagggcc aaaccccgcc ggccgcctgg
      121 gaaaagatcg ccattaccgg caaggccaag tcggccatcc ggcgcgccgc gcgcgatgcc
      181 ttgcgccggc aatactccga gctcgggcgc catctcttga tcaccgcctt tgagcgtgcc
      241 ggctatgagt acagcgacga ccgcttgaaa aaggccctga tgcgcctgtc gcaaaaatcg
      301 gtcgaggagg tgcacgccac cgtcggccgc ggcgaattgc ccgtgcgcga tgtgattcgc
      361 gccgtcgtgc ccgacgccga cttggcgagc gagccggcgc cgcgcaaacg caaccgccgc
      421 gagcgcggcc agggcgagga gggctggttc aacctgtcca aggtcttcaa tttgaagttt
      481 cgctggccgg gcagcggcag cggcgaggcc aggcaaaccc aggtgctgcc gatccgcggg
      541 ctcagaaacg acgtacccgt aacctttgag gaaggcggtg ccgtgcccgg cgatcgcatc
      601 gtcggcgtgc tgtcggaagg cgagggcatc cgcatcttcc agatccattc gccgaagctc
      661 aaggagtacg agaacgagcg ctggatcgac gtcacctggg atgtcgatcc cgagcgtccc
      721 gagcgctttc ccgccaagat cttggtcacg gcgctgaacg agcccggcac cttggcccag
      781 atcgcccagg tgatcggcga gtcgggcggc aatatcgaca acgtgcgcat gatccggcgc
      841 gccaaggatt tcacggaaat gctgatcgaa gtggaggtgt gggatcttga ccacctgacg
      901 cacatcatga atggggtcaa aggcaagcct gtcgtgagca gcgtcgagcg cgtcttcgtc
      961 tagcgacgct ggacgaaatg ggctttagat ccgtgccgcg gcgcgcacgc tcgctccccg
     1021 acagcagcca agcttaagaa gcgactgccc gcagccgccg ccacaatctg ccgcctaaga
     1081 ctgcttaaag gacttgaacg acaccgccgc gagaaagaga tggaacgctc aaccccaacg
     1141 cccgccgccc acctgcgcct cggcgtcaac atcgatcacg tcgccaccat tcgcaacgcc
     1201 cgcggcggcc gccatcccga tcccctgcgc gcggcgcatt tggcggtgga ggcgggcgcg
     1261 gatggcatca ccgcgcacct gcgcgaggat cgccgccaca tctccgatgc cgatatcgcc
     1321 cgcctcaagc acgagctgac cttgccgctc aacctcgaga tggcctcaac cgaggagatg
     1381 ctggccatcg cgctccggca tgtgccgcac gcctgctgcc tggtgccgga aaaacgcgag
     1441 gagcgcacca ccgaaggcgg gctcaacgtc gaggccggcg agaaccacct caggcgctat
     1501 gtcgacgaac tcaaggccgc cggcattcgc gtctcgctct ttatcgagcc cgagctcgcc
     1561 gcggtcgcag cgtcggcacg gctcggcgcc gacatcgtcg agttgcacac cggagcttat
     1621 tgcgagcggg cgctggagga tgaccccgcc ggcagcaagc gcgaattgga ccggctcaag
     1681 cgcgccgccg cggcggccgc cggcgccggc ctcgaggtgc acgcgggaca tggtctcacc
     1741 ttcaacacgg tcgccgccgt cgcccgcctg ccgcaggtgg tggagctcaa catcggccat
     1801 ttcctggtcg gcgaggccat cttcagcggg cttggcccgg cgattcgacg catgcgcact
     1861 ttgatggacg aggcccgcta cggcatcgcg ccgcggcagg ccgccgcggc gacgtcatga
     1921 tcctcggtct cggcaacgac atcatcgaca tcaggcgcat tcagcgcacc atcgaccgct
     1981 acggtgagcg ctttcttggc cgtgtcttta ccgacacgga acggcgaaaa tccgatgcgc
     2041 gcgccaatcg tgccgcctcc tacgccaagc gctttgccgc caaggaagcc tgcgccaagg
     2101 ccctgggcac cggcctgcgc ggcggggtgt tctggcgcga catgggtgtt gtcaatttgc
     2161 gctccggccg gccgaccatc gagctcaccg ggggggccgc agcgcagctc aagcgcctca
     2221 cgccacccgg ctttgcggcg cgtatcgacc tgacgctgac cgacgacttt ccccaggccc
     2281 aggccgtggt gctcatttcc ggcgtgcccg ttgcaaggag cgaaaatgac gcgggataag
     2341 acggccgccg ttagccagtc cgtgaattgc cgcctgcccc acaaaccgct atacgggtcc
     2401 ggaaagaagg ggaccggcat gcttcttcgg ctagcggcga gaagcagcag cagaaagaga
     2461 gagcatgagt tccgagaccg aagctggcaa ggtgagggaa tccggctggc gcgaaacgat
     2521 caaaatcgtg gttcacgccc tgattttggc gatgatcgtg cgcattttct tctaccagcc
     2581 cttcaacatc ccctccggct caatgaaaca gacgctgctg gtcggggact atctgttcgt
     2641 ttccaagctg gcatacggct atagccgcta ttcctttccc tggggcatcg ttcccatcaa
     2701 cggccgcatt ttcggcaccg agccaaagcg cggcgatgtg gtcgtcttca agctaccgcg
     2761 cgacaattcc acagatttca tcaaacgcgt gatcggtttg ccgggggacg agatttcggt
     2821 ccgcaacggc gtgctttata tcaatgggca ggccgtgccg aaggtgcgca aaggcgattt
     2881 tatttctccc gaggaggatc gaccgatccc gcgctttgag gaaacacttc ccaatggcgt
     2941 caaatactac gtgctcgatt ctacgcctaa tggccaattc gacaacgtcg gtccctataa
     3001 ggtgccggcg ggacattatt tcatgatggg cgacaatcgc gacaattcga ccgacagccg
     3061 cgagcagtcg ccgcggttcg gggtcggtta tgtgcctttt gagaatttgg tggggcgcgc
     3121 cgagatcatt ttcttctccg ctgccgtcga tgaccccaac gccttccggc tgacgcaacc
     3181 ctggcactgg cccatggaca ttcgttggaa ccggattttc agcctcgtgc gctagggccg
     3241 tggcgccggc ccgcaagctc aaggaactcg agacgcgcgt cggctatcat ttcaaggatc
     3301 gcgccctctt acgccgcgcg ctcacccatg ccagcgtgcg ccaggcctcg gccaagcgcc
     3361 gcgacaacga gcgcctcgag ttcttgggcg accgcgtgct ggggctggcc gtcgccgagc
     3421 tcttgaacga aagctatccc agcgccagcg aaggcgagct cgcgcgcctt tacaatcggc
     3481 tggtccgcgg cggaacctgt gccgaaatcg cccgcgagct taacctcggc acttgcctgg
     3541 tcttgagcga atcggaagag ggcagcggcg gccgcgacaa ggaaaccatc ctggccgacg
     3601 cctgcgaggc cctgctcggg gccatcttcc tggaggcggg cttcgagaag gtgcgcgacg
     3661 tggtgcgcgc ccactggagc ggccgcctgg ccaaagcccc cgccgaggtg gccgacgcca
     3721 aatcggccct gcaggaatgg gcgcaagggc aaggcctcga gctgccgcgc tacgtggaag
     3781 tggaacggct cgggcccgat cacgcccccc gcttcaccgc cgaggtcagc atcaaaggca
     3841 aaaaggccgc gcgcggggag ggcgccagca agcgcgcggc cgaacaggcc gccgccgccg
     3901 cgctgctgtc gcgcgagggg gtgtggagac cggcaaagtg agcgcctcgc ccgcaagcga
     3961 aacgcgctgc ggctttatcg ccgtgatcgg cgcccccaat accggcaagt cgacgctcgt
     4021 caatgcgctg gtcggcgcca aggtcgcaat cgtcagccac aaggtgcaga cgacgcgcac
     4081 gcccttgcgc ggcatcgcca tcgagggcgc gagccagctg gtgttcatcg acacgcccgg
     4141 cctgtttcag ccgcgccggc gcctcgagcg cgccatggtc gaggcggcct ggagcggcgc
     4201 cgaggacgcc gatctcatcg ccctcttgct cgatgccagc cagggcctcg acgcggcggc
     4261 gcagcgcatc ctcgacgagc tggcggccaa gacgtcctca ctcgtcctgg ttttgaacaa
     4321 gatcgatcgc gtgcccaagc cgcgcctgct gacgctggcc gcggaagcca acgcccgcct
     4381 gcccttcgcc gccaccttca tggtgtcggc gctcaagggc agcggcgttg ccgaccttaa
     4441 gcgctacttc gctggccagg ttaagcccgg tccttggcat tttcccgagg acgaaatctc
     4501 cgatgcgccg ttgcgcctgc ttgccgccga gattacacgc gagaagatct acgcgcgcct
     4561 gcacgatgaa ctcccctatc aaaccgcggt cgaaaccacc gcctggcagg agaggaaaaa
     4621 cggcgtgcgt atcgagcaga ccatttttgt ggagcgcgac agccaaagga gcatcgtgct
     4681 gggcaagggc ggccagatga tcaggcagct gtcgatggag gcgcgccagg agctggccca
     4741 gatcctcgag caacccgtgc acctgatctt attcgtcaag gtgcgccagg actgggaaaa
     4801 cgatcccgaa cgctaccggc agatggggct gaggtttccc aagacttgaa gtagagcccg
     4861 ccggcagaaa gccccgccat ggaatggtcg gatgaaggca tgatcctcag cgtgcggccg
     4921 cacggcgaga ccggcgccgt gcttgagctc ttcacgcgcc gcaacgggcg tcacctgggc
     4981 ctcgtgcacg gcggtcgctc gcgcaagctg cgacccattc tgcagatcgg caaccacgtt
     5041 gaggcgagct ggaaggcgcg cattgccgac aacctcggcc acttcgcctt ggagttgcgc
     5101 aaaagctacg cggcgctggt gctcgatgat gccgccgccc ttgccgcgct cacctccatc
     5161 gccgcgctgg cccgcctgct gcccgagcgc gatccacacc cgaacctgtt cgaggtcacc
     5221 ttgttcgtgc tgggctacct cgacgagcgg gaggtgtggc cggcgctgct cgtgcgctgg
     5281 gagctggcgc tgctcgagga actgggcttc ggcctcgatt tgacgacctg tgcggccacc
     5341 ggggtcaaga ccaacctcag ctacgtgtcg cccaagtcgg gtcgcgcggt gtccgccgcg
     5401 gcgggggagc cctaccaggc gcggctgctg gcgctgcccc ccttcctgct ggggcccgcg
     5461 cccgaaggcg tcacggccgc cgacgtggcc gccgggctgg ccctgaccga gcatttcctg
     5521 ctggcgcgcg tgctgcggcc ccgcgacctc gccatgcccg aggccagaaa ccgcttgttg
     5581 tcttaccttc ggccgaatgt gtagctatcg agccccgtcg ccttaaccgg gccgctgggc
     5641 ccgttcactg acctcctgca ctgaactcgt tcactgggcc ccgttcagtt ggcccgctca
     5701 cccggctcgc aaggactaga aaaccccgtg ccatccctca aatcttggcg tcaccatccc
     5761 gcgcccacca tggcggccct cggcctcgtt tcgggtatcc tgtccgccgt cgtcggcttt
     5821 gaggtgcgca tcggcgtgct ggattggccg gggacggtct tcttcctgtc cgccgaaatg
     5881 atgccgatcg gcctcttgtt cgccgccgtg atggcctttg ccatcgccgc ctggcacgcc
     5941 cccgcctggg ccccgattct caccttcatc ggcacgcttt atgcctggag tgcggcgatc
     6001 cagaccgcgc tctttatgca taggatcggc ggcggcgatg ccagccacgc cgggccgctg
     6061 ctggggggcg tgttcgccgg cctcgtcggc gccgggctca gccacctgtc ggtggcgctg
     6121 tttgcgcccg agctgcgcga tctcggccag gcgatgcgaa ccgccctggt gggcgcgctc
     6181 gccggcgtgc tgttttattt cggcagccgc aacctgatcg acgcccgcat tctgtttctg
     6241 gtctggcagc cggcggtcgc ctacgccatc ggcctcggcc tggcagccag gcccgcaccg
     6301 caggcctcat aaggcggcaa aagcgccacg gcgcgcgcta gagcagcgcg ccgccggcag
     6361 aaagtccggg tggcgctggc ggcgctactt gcggccacgc tcgaccttga tggcaccgcc
     6421 cgcgccttgc gtcagcacga aggtgtcgcc atcgcgcggc tccaactgcc gggcggcgga
     6481 cccggccttg tcgatcttga ggggaccggc cgcgcagctg gtgtggttga cgctcgcccg
     6541 cgccggcaag gtctcgctct cttccccgag ggtgtaggtg accgccgccc cggtgcggtt
     6601 ctcgatcgcg aagctcactt tcagcgcctc gggccgaccg aacaactgta cggagatgaa
     6661 tttcggctgc cggcctgccg cccgcgccac gccgacgccg atctcggtgg cgctcgcctc
     6721 cagcaggttg aggcgatgcg ccggcgagct cttccagccc tcgaccacgt cacgggcgag
     6781 ctggctggtg gcaaaaccgc ggctgtccaa gttgaaggcc aggttttcgg cgaccaggca
     6841 gtagcgatag ccctgcgcct cggcccgttg gtgcggctcg cgcccgtcgg cctcatgcgc
     6901 gaatttgccc gtgcggccga gatagtcggc gaacgcgcgc gccgccgcga tcagcgccgc
     6961 gttgggcttg agcgtgccca acttgtgttg cttgcgaaag gcgttcgtca tctcgacgat
     7021 ggcaagctcg gtctggggca ggtcgggaag cacgggcatg gcatagtttc caaggccggg
     7081 cgcagcgcgg cgtgccccgg cattcaaccc cgaattgcgc gcgcctggcg ggtttgggcg
     7141 cggcacggcc gatatcggcc ataactgcgg cgctgagcgc aaggggcggc gcgcccaaga
     7201 tccaccgcca aacccgccaa ggcctgcccg ccatcgcttg agcccgcccg cgtcctgggc
     7261 taacccaagg cgcatgagca aaaccacgat tctcgccccg ccgccgccca tcgagcccat
     7321 taaactcaag gatgcgctgg aagagcgcta cctcgcctac gcgctctcca ccatcatgca
     7381 ccgggcgctg cccgacgtgc gcgacggcct gaaacccgtg catcggcgca tcctttatgc
     7441 catgcgcgag ctcaggctcg atccggcggg cggctacaag aaatgcgcca agatcgtcgg
     7501 cgaggtgatg ggcaactatc acccgcatgg cgatcaggcc atttacgacg cgctggtgcg
     7561 gctggcgcag gattttgccg tgcgctaccc gctggtcgac ggccagggca acttcggcaa
     7621 cgtcgacggc gataacgccg cggccatgcg ctacaccgaa agccggctca ccaatgtggc
     7681 cgcgctgctg ctcgagggca tcgacgagga caccgtcgac ttccgtccca cctacgacgg
     7741 cagcaaccag gagccggcgg tgctgccggc caacttccca aacctgcttg ccaacggcac
     7801 ctcggggatc gccgtcggca tggccaccag cgtgcccccg cacaatgtcg aggagctctg
     7861 caacgcgcta ttgcatctca tcaagcaccc gaacgcccac gtcgagaagc tggtcgagtt
     7921 cgtgcccggc ccggactttc ccaccggcgg ggtgatcgtc gagccgcgcg atgccatgct
     7981 cgaggcctat aagaccggtc gcggcggctt ccggctgcgc gcccgctggc agcgggagga
     8041 gggcggacgc ggcacctacc agatcgtcgt caccgaggtg ccctaccagg tgcagaaggc
     8101 caagctggtc gagcgcatcg ccgaactcat caacgacaaa aagctgccgc tgctgggcga
     8161 cgtgcgcgac gaatcggccg agaccgtgcg gctggtgctg gaacccaaga gccgcagcgt
     8221 cgatccggtg ctgctgatgg aaagcatgtt ccgcctcacc gagctggagg tgcgcttttc
     8281 actgaacatg aacgtgttgt cggccggaca agtgcccggc gtgctgtcgc tgcgcgacgt
     8341 gctgcggcgc tggctggagc accgccaagt ggtgctcgtg cgccgctcca agttccgcct
     8401 ggaaaagatc gaacaccgtc tcgaggtgct cgacggctac ctgatcgcct acctcaacat
     8461 cgacgaggtg atccgcattg tccgcttcga ggacgatccc aaggccaagc tgatcaagcg
     8521 cttcaagctc accgaggtgc aagccgacgc cgtcctcaat ctgcgcctga aatcgctgtc
     8581 gaagctggaa gaggtcgaga tccgggcgga gcacgacaag ctgagcaagg agcggcgcgg
     8641 ccttaaatcg ctgctcaagc acgaggacga gcagtgggac cgcatcgccg acgagatcaa
     8701 ggcaacgcgc gagacctatg ccaagaagac cgagctcggc cgccgacgct cctccttcgc
     8761 cgacgccccc agcatcgaga tcgacctcga gcaggccatg atcgagaagg agccaatcac
     8821 ggtgatcctg tccgagaagg gctggatccg cgccatgaag ggccacctgg aggacgtttc
     8881 caagctggag ttcaagcagg gcgacgcctt gaagcgggcg gtcaaggcct cgaccaccga
     8941 ccggctgctg ctgctcgcca gcaacggcaa ggtgttcacg ctggcggccg accagctgcc
     9001 gggaggccgc ggccacggtg aaccggtgcg gctgatggtc gacctggagg aaaaccatga
     9061 cttcgtcgag atctggatgc atgaagcggg ccgcaaactg ctggtgggtg cgacctctgg
     9121 ccacggcttc atcgtgcccg aggacgagat ggtcgccatg acgcgcaagg gcaagcaggt
     9181 gatgaacgtc gattcgcccg ccgaggcgtg ctgcgtcgta ccggtcgagg gcgacctggt
     9241 ggccgccatc ggcgagaacc ggaagatgct gatcttcccg ctcgaggagg tgaacgaaat
     9301 gacgcgcggc aagggcgtca ttttgcaacg ctacaaggat ggcagcctgt ccgacctgcg
     9361 cgtcttcaaa aaggccgatg gcttgagttg gctcgatgcg gcgggacgca ccttcacgct
     9421 gcccatggcc gaattgcgcg actggctggg ccagcgcgcc caatccgggc gcctggcccc
     9481 caagggattt ccccgctcca acaagttcgg tcccgccttc tagcagtccc aaaagggcca
     9541 acagcgcagg atagctgcag gcgctggaga cggacggtgg acttggggtg tggcgtgatg
     9601 agggttgctc gagcgaggtg ccgattggct cgtgcctgtg gaccggggca cagggcgtaa
     9661 gtgcagaggg cttgagtgca gagggcttgg tgcaggggct aggtgcccgg ggctaggtgc
     9721 ccccggggct aggtgaccga ggctagcggc gcgggcggcg aggcggggcg aagaggtgag
     9781 acatgcgggc aggcctggtc gggttgatcg cgctcgtgct ggcgcttgcg cgcgccccgg
     9841 gcgcgttcgc cgagacgccc tgcaacaagg ccgactttga ggccgtggtg gatgaggccg
     9901 cggcggcgct gcgcagcctg aaccatgaaa acacccccca gttccaggcg cggctgcgcc
     9961 agctcaagga caagcgcggc tggagccacg agcaat
//
LOCUS       DASTMT010000094         1800 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_3004205, whole genome shotgun sequence.
ACCESSION   DASTMT010000094 DASTMT010000000
VERSION     DASTMT010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 1800)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1800)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1800
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3004205"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            91..>1800
                     /locus_tag="VFR00_01945"
     CDS             91..>1800
                     /locus_tag="VFR00_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019198738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HEU0158040.1"
                     /translation="MRPFLTLQHPAQAQRYYAQGHWRQDTFYGLLARHAAARPAAIAL
                     VDGRRRLSWAELLSWVDGVGADLRARGLKGGDRVCLLLSNRVEAVVAFLACAREGLAC
                     NPSLHRSHTWSEVFSLVQRLSARALIAEPGWGAAPSHLEAGTASLACNPSLHRSHTWS
                     EVIGLVQRLSARALIAEPGWGAAPSNLEAATASIASLRVVYDLARFPKAGDNGGAASC
                     DPDRVVYLAFTSGTTGTPKCVMHSHNTLLANARDLVADWGHGPETVLLSLSPLSHHIA
                     WVGVAQWLVAGCQFVCNDPPRAGSALDWIVETGATYVMGVPTHAMDVLAEQTRRRLPR
                     LGRVAIFYMAGAPIPPSVAAAFVAQAITPQNVYGMTENSSHQYTHPGDATQTIVATCG
                     RGGDGYQVALFDPTDRDRPAAPGQMGEIGGRGPALMLGYFDNQHATETSFNSDGWFMS
                     GDLGSMDAHGNLTVEGRLKDLIIRGGHNIYPAHIEALALRHGQVGRVACFAVADARLG
                     ERACIAISGSAPAHDLLEHLGKEGLSTYDMPEYFVRIAEFPLTASGKILKRELVEMVK
                     RGEL"
BASE COUNT          279 a          606 c          615 g          300 t
ORIGIN      
        1 gcgtgcaagg gctcgggcga accgggtggc gccgcctgcc ggcgcggcct gacgagggaa
       61 aggctgtgtt gccaaacaag gaggtgcgcg atgcgcccat ttctcaccct gcagcatccg
      121 gcccaggcac agcgctacta cgcgcagggc cactggcggc aggatacctt ttacggcctg
      181 ctggcgcgtc atgctgccgc gcggccggcg gcgatagcct tggtggacgg gcggcgaagg
      241 ctgagctggg ccgaactctt gagctgggtc gatggcgtcg gcgccgacct tcgcgcgcgc
      301 ggcctcaaag gcggcgaccg ggtttgcctg ctgctgtcga accgcgtcga ggctgtcgtc
      361 gcctttcttg cctgcgcgcg cgagggcctt gcctgcaacc cgtcgctgca tcgcagccac
      421 acctggagcg aggtcttcag cttggtgcag cgcctgagcg cccgcgcgct gatcgccgaa
      481 cccggctggg gcgccgcgcc cagccatctc gaggcaggaa cggcgagcct cgcctgcaac
      541 ccgtcgctgc accgcagcca cacctggagc gaagtcatcg gcttggtgca gcgcctgagc
      601 gcccgtgcgc tgatcgccga gcccggctgg ggcgccgcgc ccagcaatct tgaggcagcg
      661 accgcgagca tcgcctcctt gcgggtggtc tatgatctgg cgcggttccc caaggctggc
      721 gacaacggtg gcgcggcctc atgcgatccg gatcgcgtcg tctatctggc gttcacatcg
      781 ggcacgactg ggacgccgaa gtgcgtcatg cattcgcaca ataccctatt ggccaatgcc
      841 cgcgatctgg tcgcggactg ggggcacgga cccgaaacgg tgctgctcag tctgtcgccg
      901 ttgtcgcacc atattgcttg ggtcggcgtg gcccagtggc tggtcgcggg ctgccaattc
      961 gtctgcaacg acccgccgcg ggcaggctcg gcgctggact ggatcgtcga gacgggggcc
     1021 acctacgtca tgggcgtgcc cactcatgcc atggacgtgc tggccgagca gacgcggcgg
     1081 cggttgccac gcctggggcg ggtggcgatt ttctatatgg ccggcgcgcc cattccgccg
     1141 tcggtggcgg cggcattcgt ggcgcaggcc atcacgccgc agaacgtcta cggcatgacg
     1201 gagaactcct cgcatcagta cacgcatccg ggcgatgcca cccagaccat cgtggcaacc
     1261 tgcggccgcg gcggggatgg ctaccaggtc gcgctctttg acccgaccga ccgggaccgc
     1321 cccgccgccc ccggccagat gggcgaaatc ggcggccgcg gcccggcgct catgctcggc
     1381 tatttcgaca accagcacgc caccgaaacg agcttcaata gcgatggctg gttcatgtcg
     1441 ggcgaccttg gcagcatgga cgcgcacggc aatctcacgg tcgagggcag gctcaaggat
     1501 ctcatcatcc gcggcggcca caatatctat ccagcacaca tcgaggcgct ggccctgcgc
     1561 cacggccagg tcgggcgcgt cgcctgcttt gccgtggccg atgcgcggct gggcgagcgc
     1621 gcctgcatcg ccatttcagg gagcgcgccg gcgcacgatc tcctcgaaca tctcggcaaa
     1681 gagggtctct cgacctacga catgcccgag tatttcgtgc gcattgcgga gtttccattg
     1741 accgccagcg gcaaaattct caagcgtgag ctggtcgaaa tggtaaagcg tggggagctt
//
LOCUS       DASTMT010000095        10736 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_4621323, whole genome shotgun sequence.
ACCESSION   DASTMT010000095 DASTMT010000000
VERSION     DASTMT010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 10736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10736
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4621323"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..812)
                     /locus_tag="VFR00_01950"
     CDS             complement(<1..812)
                     /locus_tag="VFR00_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018453371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU0158041.1"
                     /translation="MADVLDEPRARAEPQSKLKVIDCDIHPSLKSRADLNPFLSKRWQ
                     QHLETYGGHLRTPFTSTTPYPRSSPLLARRDAWPPSGGPPGSDVAFMRQQHLDPINVE
                     FGILQVLDLLVVSQQNQELGIALQRAINEWQIECWSKPEPRLKASIVVGQDNTEAAVA
                     EIERCAATDKYVQVNVSPRANEPLGRRRYWPLYAAAEAANLPLGIHVGGYGGHAPTGS
                     GWPSYYNEEHHSNAHSVAGQLTSLVLEGVPERFPKLKFVFIEGGFGWVPALT"
     gene            complement(828..1880)
                     /locus_tag="VFR00_01955"
     CDS             complement(828..1880)
                     /locus_tag="VFR00_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014491841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU0158042.1"
                     /translation="MRIDCDIHPGLEGTRTTLLPYLDAHWQEQVLSRGIDGLDLMSYP
                     PNMPLCCRPDWRPKRGKPGSDFELLKAQALDAFDSSHAICNVLYGAQAVFDPYMAAAF
                     CRAINDWIAAEWLARDPRLRASIVVPMQAPDLAVEEIMRLAGDRRFVAVLVLVQGETL
                     LGRRQFWPLYAAAQQHKLPIAIHSGSQYRMAPSSIGWPSYRFEYYIAEAQAHQAQLLS
                     LILEGVFAKFPDLKVVMMESGISWLPAFMWRANKTWKGVRVEVPWVDRPPADIMRDHV
                     RLTTQPFDAPPGARDLEQLLDQIGSDRMLLFSSDYPHWQFDGDCPIPKGFPASLVARM
                     CVDNPLETFPRLQASP"
     gene            2164..2394
                     /locus_tag="VFR00_01960"
     CDS             2164..2394
                     /locus_tag="VFR00_01960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0158043.1"
                     /translation="MQCPVCARPAENLTPNTLDGVVVGCDHCGSYRIHGGTFHAFVSL
                     EMTQRLAALEAAKLASQGGWPLVNNRCLTARL"
     gene            2636..3322
                     /locus_tag="VFR00_01965"
     CDS             2636..3322
                     /locus_tag="VFR00_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013168040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2161 family putative PD-(D/E)XK-type
                     phosphodiesterase"
                     /protein_id="HEU0158044.1"
                     /translation="MESSLYLPVKRFLEGLGFEAKGEICGCDLVALRQGEPVAVVIGE
                     LKLTFTLDLVLQAVDRTTACDEIWLAVRASRRGRGRETDARVKRLCRLLGFGLLCVTS
                     ASKVEVIVEPVPWRPRRDAKRRSQLVDEHRRRRGDPAAGGSVRQPIMTAYRQQALACA
                     AVLATGPSRLGVLKLGVPDAPKILQRNVYGWFVRLERGVYALTEAGRAALLRWEAQVP
                     PVTAPVAVAN"
     gene            3571..4176
                     /locus_tag="VFR00_01970"
     CDS             3571..4176
                     /locus_tag="VFR00_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008965418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxychromene-2-carboxylate isomerase"
                     /protein_id="HEU0158045.1"
                     /translation="MIEFFFDCSSPWTYLAFHNVMPLAREFDVAITWRPILVGGIFNS
                     INPSVYHSREKPVPAKVRYGRKDLADWAGLAGLKIIRPTIFPVNSVKAMRGCILLEAD
                     GKLVAFARAVFEAYWGEDQDISDERVLGDICTRLGVDRLAFLAALAEPALKSKLKDNT
                     DELIERGGFGSPTMFLDHDDMYFGNDRLILLRAALARCQTA"
     gene            complement(4285..4659)
                     /locus_tag="VFR00_01975"
     CDS             complement(4285..4659)
                     /locus_tag="VFR00_01975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0158046.1"
                     /translation="MRWALPILCLFAAGCSLPAAILPGSGTPSDERVGGGIVKIVLPK
                     GALADCQSVDDCTLVRAAEATRRAGGTHFALLPGHGGSTQKGYAYIKVFTLAPGEGAP
                     SGTMSVEEALHFFRRQPVQAAS"
     gene            complement(5276..5989)
                     /locus_tag="VFR00_01980"
     CDS             complement(5276..5989)
                     /locus_tag="VFR00_01980"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEU0158047.1"
                     /translation="MRWGLLALITLASWAGVPSAQVLAQAPAGPPPVPDGPRYVVTYL
                     EVMPTQTAQAKKLVRQFRDATQREPGNLRAEALQRIGWPNQFVLLDAWKDPGSAEGHG
                     KAATTGEFRDKIKAIQNAPLDERTHFPLSVASLPAKSGRSAVAAVTHVDVIPPQRENG
                     TALTKQLAEDGRKDDGNLRFEAVVQTNRQNHFTVIELWRDRKAAEAHSMAAHTRAYRE
                     KLAPASGALYDERFYKALD"
     gene            6516..9140
                     /gene="ligD"
                     /locus_tag="VFR00_01985"
     CDS             6516..9140
                     /gene="ligD"
                     /locus_tag="VFR00_01985"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008967757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase D"
                     /protein_id="HEU0158048.1"
                     /translation="MPKNKLTLYQQKRDFTRTEEPSGRRKIAAAEYPRFVIQEHAARR
                     LHFDLRLEVDGVFKSWAVTKGPSLDPSAKRLAVETEDHPLDYGDFEGTIPQGEYGGGT
                     VMLWDRGFWTSDEDAAKALRKGELRFTLVGEKLQGAWVLVRLRRDREGSKRNNWLLIK
                     RHDGYEREGDGEGILSQDRSVASGRSMEEIAAGKGASPKAFMLADKGAFSAKAIWHSN
                     RQDGGARPSSLVASPRARLRSSETRPRSALPNFIAPQLCETVTRPPAGQDWVHEIKLD
                     GYRLQLRVEAGRAELRTRKGLDWTGKFAPIAAAARGLGDCIIDGEVVALDRNGAPDFS
                     ALQAVLSEGRAEELIYFAFDLLFAQGEDLRGLSLEARKERLNRLLRRKGAHSRQIKYL
                     EHLAEPGDTVLKSARDLNLEGIISKRTSAPYASGRTDSWRKIKCRGGQEVVIGGWSGN
                     ANHVRSLLVGVYRGDDLVHTGRVGTGFNARNAGNLLKKLAALKTNRSPFTGRNAPRRQ
                     ADWTWVKPELVAEIEFAGWTGDGNVRQAAFKGLREDKPASEIRAEHPKADAMTGLPSS
                     KLGAARGKGGSEVIMGVTLSKPDKPLWPAPESYTKRDLARYLETVGPWMIEHLRGRPC
                     SIIRAPDGIEAERFFQRHEMKGMSQLISAVRVAGDRKPYIQIDRVEGLIAAAQIAGIE
                     YHPWNNEPGHPSVPGRLVFDLDPAPDVAFDQVVGAAKEMRTRLEALGLVTFCKTTGGK
                     GLHVVTPVTVHDQDALGWDEAKAFAEAVCAAMAADSPDRYLLNMSKKLRTGRILLDYL
                     RNDRMSTAVAPLSPRMRAGAPVSMPIAWSQVRAGLDPQRFTMATAPAQLAKTKAWDGY
                     ESASAPLKAAIRKLVGRR"
     gene            complement(9205..9666)
                     /locus_tag="VFR00_01990"
     CDS             complement(9205..9666)
                     /locus_tag="VFR00_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015319209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEU0158049.1"
                     /translation="MLVGVISDTHGLLRPEALAALRPSAMILHAGDVGKPAILERLSE
                     IAPVVAVRGNIDVQAWASCLPETAVVSAGNHKILVLHDATQLSVDPVAAGLKAIVCGH
                     SHKPSIEKRAGILYLNPGSAGPRRFRLPVTLAHIHVSTTSLEAEIIPLPMA"
     gene            complement(9673..10512)
                     /locus_tag="VFR00_01995"
     CDS             complement(9673..10512)
                     /locus_tag="VFR00_01995"
                     /inference="COORDINATES: protein motif:HMM:NF014759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HEU0158050.1"
                     /translation="MRRPLLIIDGDSFAHRAYHALPKSIRRRGNKGAGAILGFANFLL
                     RLYQTEAPRAVLVGWDTLDEPTYRHEALASYQAGRSFEEELTDQLEVLPGFVTACGFA
                     CAKAAGYEADDFLAAAVAREEKRGGTALVASGDRDAYQLVSEHTTILQPIRAGEMARI
                     GPAEVRRRYGVEPKQVPDFIALRGDASDRIPGAAGIGAVGAASLLRKYGTLEKALAAG
                     RLAALGDKLRLYRSIATMDKAAPLPPLRNQKPTWGKAALLARDWQLKKLAERLDGLAA
                     KAR"
BASE COUNT         1907 a         3397 c         3556 g         1876 t
ORIGIN      
        1 caggtcagtg cgggtaccca gccgaagccg ccctcgatga agacgaactt gagtttggga
       61 aatcgctcgg gcacgccttc cagcaccagg ctggtcagct gaccggccac agaatgagcg
      121 ttcgaatggt gctcttcgtt gtagtaggag ggccagccgc tgccggtggg cgcgtgaccg
      181 ccgtagccac cgacatggat gccgagcggc aggttggctg cctcggcggc ggcgtagagc
      241 ggccaatagc ggcggcgacc caggggctcg ttagcgcgcg gggagacgtt cacttgcaca
      301 tatttgtccg ttgccgcgca gcgctcgatt tccgccacgg ccgcttcggt attgtcttgg
      361 ccaaccacga ttgaggcctt gaggcgcggc tccggcttgc tccagcattc gatctgccac
      421 tcgttgatgg cgcgctgcag cgcgatgcca agctcctggt tctgttgcga gacgaccaac
      481 agatcgagca cctgcaggat cccgaactcc acgttgatgg ggtcgaggtg ctgctggcgc
      541 atgaaggcga cgtcagagcc cggcggtccg ccgctcggtg gccaggcgtc ccggcgcgcc
      601 agcagcgggg aggagcgggg gtagggcgtg gtcgaggtaa agggcgtacg caggtggccg
      661 ccataggtct ccagatgctg ttgccagcgc ttgctcagga aggggttgag atccgcccgg
      721 cttttcaggc ttggatggat gtcgcaatcg atgaccttca gtttgctctg gggttcggcc
      781 cgggcacggg gctcatcgag aacatcggcc attggcgcct ctcgttttca aggtgacgct
      841 tgcaagcggg gaaacgtttc cagcgggttg tcgacgcaca tgcgcgccac caggctggcc
      901 ggaaatccct tgggaatggg gcaatcgccg tcgaattgcc agtgcggata gtcgctggaa
      961 aaaagcagca tccgatcgga tccgatctgg tccaacagct gctccagatc ccttgctcca
     1021 ggtggcgcgt cgaagggttg cgtggtgagg cgcacgtggt cacgcatgat atcggcgggc
     1081 gggcgatcga cccaagggac ctccacgcgc acgcccttcc aggtcttgtt cgcgcgccac
     1141 atgaatgccg gcaaccagct gatacccgat tccatcatga cgaccttgag gtcgggaaac
     1201 ttggcgaaca cgccctccaa aatcaggcta agcagctggg cctgatgcgc ctgggcctcg
     1261 gcgatgtagt attcgaagcg gtaggaaggc cagccgatcg agctgggggc cattcggtac
     1321 tggctccccg aatggatggc gatcggcagc ttgtgctgct gggcagcggc atagaggggc
     1381 cagaactggc ggcgtccgag cagggtttct ccctgcacca gcacgaggac cgccacgaag
     1441 cgccgatcgc cggcaagacg catgatctct tcgacggcga ggtcgggcgc ttgcatgggc
     1501 acgacgatgg aggcccgcag gcgaggatcg cgcgccagcc actcggccgc aatccaatcg
     1561 ttgatggcgc ggcagaaggc agccgccatg tagggatcga acaccgcctg cgcgccatag
     1621 agcacgttac aaatggcgtg cgagctgtcg aaggcgtcca gcgcctgggc cttcagcagc
     1681 tcgaaatcac tgccgggttt gccgcgcttg ggccgccaat cgggccggca acacagcggc
     1741 atgttcgggg gatagctcat caagtccaag ccatcgatgc cccggctcaa aacttgttcc
     1801 tgccaatgcg cgtcgagata gggcaacagc gtcgttcgcg tcccctcaag ccccggatga
     1861 atgtcgcagt caatgcgcat gcctggcgtt cgcgcccaaa cctggtgtgg aagtttcacg
     1921 caaggtgact tcactggcaa cgaccaactt tctcgcgccg ggctaacgtc cgctcgagcg
     1981 actgaaccgg tttcgcgccg cgccccgccg tctcgtcgcc cgcagcacgc gcgcgcgccc
     2041 gaaacgtcgc atcaccgggg aagacgtggc ccagcggccc gggatgccga taccggcgcg
     2101 ccgatttgac aaacggccgg gtcttggcat attgtatacc agaaagcagc gtggagggtc
     2161 ccgatgcaat gtcccgtgtg cgctcgcccg gcggagaatt tgacgcccaa taccctggat
     2221 ggggtggtgg ttggctgtga tcattgcggc agctatagga tccacggcgg caccttccac
     2281 gcctttgtca gcttagagat gacccagcgt ctagcggcct tggaagcggc gaagcttgcc
     2341 tcacagggcg gctggcctct ggtcaataat cgctgcctaa cggcgcgcct ctgacactcg
     2401 cgagcgggca acgccttcgg caacgtcgtt tgccggagcg cttggtgcgc gcctggtggc
     2461 ttgtggccgc ctccatcaca agttgcttgg ccggttacgg tcgcctttca cgccccgctt
     2521 ctttacgcat gccttaaagc caatcggcgt ctgccagagc cgccgcaagg ccctggtgca
     2581 ctcggctcga caggcgcgcg aatctcgact aggcgatggc gccgaagagg cggccgtgga
     2641 atcaagcctt tacctgcccg tcaaacgctt cctcgaaggc ctcggcttcg aggcgaaggg
     2701 tgagatctgc ggctgtgatt tggtggcgct gcggcagggt gaacccgtcg cggtcgtcat
     2761 cggcgagttg aagctgacct tcaccctcga tctggtgctg caggccgtgg accgcacaac
     2821 ggcgtgcgac gagatttggc tggccgtgcg ggcctcgcgc agggggcggg gacgggagac
     2881 cgacgcccga gtcaagagac tttgccgcct tctcgggttt gggttgctgt gcgtgaccag
     2941 cgcgagcaag gtcgaggtga tcgtcgaacc ggtgccctgg cgtccaaggc gcgatgcgaa
     3001 gcgccggtca caacttgtcg acgaacaccg gcgtcgtcga ggcgatcccg ccgcgggcgg
     3061 cagcgtgcgc caaccgatca tgacggctta tcgccagcag gcgctggcgt gtgcagcggt
     3121 gctcgccacg gggcccagcc gccttggcgt gctcaaactc ggcgtgcccg acgcgccaaa
     3181 aatcctgcaa cgcaatgtct atggctggtt tgtccggctc gagcggggtg tttatgcgct
     3241 gaccgaggcc ggcagggccg cactcctgcg gtgggaggcg caagtgccac cggtaactgc
     3301 gcccgtcgcg gtcgcgaatt gaagtgtgca ctcggttagc cgaagaagcg aagccgcgtg
     3361 ccttcgggcc atctcccggg cttgggcccc aagggcaacg ggcgggggcg gcgcgacctc
     3421 catgcgagcg ccgcacccat cttaacgaga gggatacgct gggctcgatt gtggccaagc
     3481 cattgacacg gggcgagtga gcggcgacgc caatgaaggc ggcagcgcgc ccaggcgagg
     3541 gcttgcgcaa ggggccccca ggaggacgag atgatcgagt tcttttttga ttgttcgagc
     3601 ccttggacct atctcgcctt tcataacgtc atgccgctgg cgcgcgagtt cgacgtggcg
     3661 ataacctggc ggccgatcct ggtcggcggc attttcaaca gcatcaatcc cagcgtctat
     3721 cactcgcgcg aaaaaccggt ccctgccaag gtgcgctacg gcaggaaaga tctcgccgac
     3781 tgggccggcc tggcgggact aaagatcatc cggcccacga ttttcccggt caacagcgtc
     3841 aaggccatgc gcggctgtat cttgctcgaa gccgatggca agctggttgc cttcgcccgc
     3901 gccgtgttcg aggcctactg gggcgaggat caggacattt ccgatgagcg cgtgctcggc
     3961 gacatctgta cgaggcttgg tgtcgatcgg cttgcgtttc tcgcggccct ggccgagcca
     4021 gccctaaaga gcaaattgaa agacaatacc gatgagctga tcgagcgcgg cggttttggc
     4081 tcaccgacca tgttcctcga ccatgacgat atgtattttg gaaacgaccg gctcatcctg
     4141 ctgcgcgccg ctctggcgcg ctgccagacg gcttgaggcg gttgccgtcc ccttgccgtc
     4201 ccccgccatt cccccggcta agccaggcgg cacaatgccg gcgcggcggg ttgcaaggcg
     4261 ccacggccgc cgtggggcgc gcgtttaact tgcggcttgc accggctgcc ggcgaaagaa
     4321 gtgcaaagct tcctcgacgg acatcgtgcc actcggcgca ccctcgccgg gagccaaggt
     4381 gaagaccttg atgtaggcat aacctttctg cgtcgaaccg ccatgtcccg gcagcagcgc
     4441 gaaatgcgtc ccgccagcac gccgcgtggc ctccgcagcc ctcacaagcg tgcaatcatc
     4501 gacagattgg caatcggcaa gggcaccttt cggcagcaca attttgacga ttccgccccc
     4561 gacccgctcg tcgctgggcg taccggagcc gggaaggatc gccgcgggca gggaacagcc
     4621 tgcggcaaac agacaaagta ttggcaaagc ccatcgcacc ggcgctgctc ccgccaatcg
     4681 ggttggcttg cgccggagca agggccgatt cggccgatga cacaatgggg ccccctgctg
     4741 gccaaaggcc ctgagagtgc cgccatggca ggtttgtgca ccgttatcgc gccgctgcgg
     4801 caggccacgc gcatcgcgcg ccccagtccg cagcgcagga agccgttggc catcctgcaa
     4861 gggttcaaac tctaggggct cgtaaagtac gtaagcctca ccggccacat gggggctggc
     4921 ttcaactgat ggcacatctg ccgacctgga aggccctccc cgatgacctc aaagaggtga
     4981 ccagcgcgag gccggcaaag acgtgcgcca acagggcgag cagcaggtta acctcaattg
     5041 ggagtctccg cgaggcgacg ccgcctcctc tgcacgaagt caaacctggg ccattacggg
     5101 cgcggctgcc cgaggtctat gccaagtgga aggagaagtt gggcgtcgag tgctggacat
     5161 tgctcgaaga aaacatcggc gagctcggct agagccaaag ccgggctcgc gcgaacgagg
     5221 tcgcgtttgc ttgtcacaga cccgtggatc cgccgacgcg atcggccggt gggtatcagt
     5281 ccaaggcctt ataaaaccgc tcgtcgtaga gggctccact cgcaggcgcg agcttctcgc
     5341 ggtaagcgcg cgtatgcgcc gccatgctgt gcgcttccgc agccttgcga tcgcgccaga
     5401 gctcgatgac cgtgaagtgg ttctggcggt tggtttgcac cacagcctcg aagcgcaaat
     5461 tgccgtcgtc cttccggcca tcttcggcga gctgtttggt tagcgccgtg ccgttctccc
     5521 gttgtggcgg aatgacgtcg acatgggtca cggccgcgac cgctgacctg ccggacttgg
     5581 ccggaagaga cgcaaccgat agaggaaaat gagtgcgctc atcgaggggg gcattttgga
     5641 tggctttgat cttgtcgcga aattcgccgg tcgtcgccgc cttgccatgg ccctcggcgc
     5701 tgcctggatc cttccaggcg tcgagcagca cgaactggtt tggccaaccg atgcgctgca
     5761 gggcctcggc ccgcagattg cccggctcgc gttgtgtggc atcgcgaaac tgacgcacca
     5821 gctttttggc ttgcgccgtc tgtgtgggca tcacttccag gtatgtgacg acgtaacggg
     5881 gaccgtccgg aaccggcggc ggtccggccg gggcctgtgc cagcacctga gcgctcggca
     5941 ccccggccca ggacgcaagt gtgatcagtg caagtaaccc ccagcgcatg gcttccccac
     6001 tcgagtttgc tctccagcct tgtcgggcaa ggctatcggg tcgcccgcgc gagcgtcaat
     6061 ggctccccgc aacagggttg gagccgaatt tccggcgcag tctcccaagt cccctgacgg
     6121 cgtcgggcgt gaccgccaag gcaaccgagc gccggtcttc cccggggatc gaccgcgccc
     6181 gtggcacgcc atggcccggc gcgccgggcc gcaggcacga gcctaaggcc tcgcgcgctg
     6241 gtgatttccg ggcggggctc tcgctcaggc gcacccccgg cccgtttgtg ccaacggcct
     6301 cagcgcaggg ccggccgcga tgcttcgcct cgcgcgagct ccaatggttc aaacgagcgc
     6361 aactaggtaa cctcgcgcga cgatcgcgct tgtgagcggt tcgagattaa ccgcggtcac
     6421 gggtcagcgc gcaggccgcc ttccaatctt tccggcgttg gaggaacgta ggtgggccgc
     6481 cggccgttgc tgccgtgtgg tcgcaggacg catcgatgcc gaagaacaag ttaacgctct
     6541 atcagcaaaa gcgcgatttc acgcgtaccg aagaaccgag cggccggcgc aagatcgcag
     6601 cggccgaata tccgcgcttt gtcatccaag aacatgccgc gcgacgattg cactttgact
     6661 tgcggctcga ggtcgacggc gtcttcaagt cctgggccgt gaccaagggc ccctcgctcg
     6721 atccctccgc caagcgcctt gccgtggaaa ccgaagacca cccgctcgac tacggtgatt
     6781 tcgaaggcac cattccccaa ggtgagtatg gcggcggcac cgtcatgctg tgggatcgcg
     6841 gcttctggac cagcgacgag gacgcggcca aggcgttgcg caagggcgag ctccgcttca
     6901 ccctcgtcgg cgaaaagctg caaggcgcct gggtgctggt gcgcctgcgc cgtgaccgcg
     6961 aggggagcaa gcgcaacaac tggctgctga tcaagcgcca tgacggttac gagcgcgagg
     7021 gggatgggga aggtattctc agccaggatc gctccgtcgc atccgggcgc agcatggagg
     7081 agatcgccgc cggtaaaggc gccagcccca aggccttcat gctggcggac aagggagcct
     7141 tttcagccaa ggcaatctgg cattcgaacc ggcaggacgg cggcgcacgc ccaagctcgc
     7201 ttgtcgcctc gccacgggcc cgtctgcggt cctctgagac gcgacctcgc agcgcgctgc
     7261 ccaatttcat agccccgcag ctgtgcgaga ccgtcacgcg tccgcctgcg ggccaggatt
     7321 gggtgcacga gatcaagctc gacgggtatc gcctgcagct gcgcgtggaa gccggcaggg
     7381 cggagctgcg cacgcgcaaa ggccttgatt ggaccggaaa attcgcaccc atcgccgctg
     7441 ccgcgcgtgg gctcggtgat tgcatcatcg acggggaggt ggtggcgctt gatcgtaacg
     7501 gcgcaccgga tttttcggcg ttgcaggcag tcttgtccga aggtcgcgcg gaggaactca
     7561 tctattttgc cttcgacctc ctgtttgcgc agggcgagga tctgcgcggc ttgtcgctcg
     7621 aggcgcgcaa ggaacggctc aatcgcctgc tgcggcgcaa gggcgcccat tcgcgccaga
     7681 tcaagtatct cgaacatttg gccgagcccg gcgacacggt gctcaaatcc gcccgcgacc
     7741 tcaacctcga aggcattatc tccaagcgca ccagcgcgcc ctatgcttcc gggcgcaccg
     7801 atagctggcg caaaatcaag tgtcgtggcg gacaggaggt cgtgatcggt ggttggagcg
     7861 gcaatgccaa tcacgtgcgc tcgctgctgg ttggcgtcta ccgcggcgat gacctcgtgc
     7921 ataccggacg ggtcggtacg ggcttcaatg cgcgcaatgc cggcaacctc ctgaaaaagc
     7981 tggcggcgct caagaccaat cgaagcccct tcactggcag aaatgcgccg cgacggcagg
     8041 cggactggac ctgggtgaaa ccggagctgg tcgccgagat cgagtttgcg ggttggacgg
     8101 gagatggcaa tgtgcggcag gctgccttta aaggtttgcg cgaagacaag ccggcgagcg
     8161 aaattcgtgc cgagcatccg aaggccgatg cgatgaccgg gctccccagc tccaaactcg
     8221 gcgccgcccg cggcaagggc ggcagcgagg tcatcatggg cgtgacgctt tcaaagcccg
     8281 acaagccgct ttggccggca ccagagagct acacaaaacg cgaccttgcc cgctacctgg
     8341 agaccgtggg gccatggatg atcgaacact tgagggggcg tccctgttcg atcatccgcg
     8401 ccccggacgg gatcgaagcg gagcgctttt tccagcgaca cgagatgaaa ggcatgtcgc
     8461 agctgatttc ggccgtgcgg gtcgcgggtg accgtaaacc ctatatccag atcgaccgtg
     8521 tcgaaggatt gatcgctgcc gcgcagatcg ccggcattga atatcatccc tggaacaacg
     8581 agcccggcca tcccagtgtt cccggccggc tggtgttcga tctcgatccg gcccccgacg
     8641 ttgccttcga ccaggtggtc ggcgcggcca aggagatgcg cacgcggctc gaagccctcg
     8701 ggctcgtcac cttttgcaag acgacggggg gtaagggact gcatgtggtg acaccggtca
     8761 cggttcacga ccaggacgcg ctgggttggg atgaggcgaa ggcattcgcc gaggcggtct
     8821 gcgcggcgat ggccgccgac agcccggacc gctacctcct caatatgtcg aagaagctgc
     8881 gcaccgggcg catcctgctt gattatttgc gcaacgatcg aatgtcgacg gcggtcgcac
     8941 ctttgtcgcc gcgcatgcgg gccggggcac cggtttcgat gcccatcgcc tggagccaag
     9001 tgcgggctgg tctcgatccg cagcgcttta ccatggcgac ggcgcccgcg cagctggcca
     9061 aaaccaaggc atgggacggc tacgagagcg cgagcgctcc cctcaaggcg gccatccgca
     9121 agttggtcgg gcgccgatag cgcctgcaac cacgtttgcc cccgcgccca ggccgatgcc
     9181 ggtcccctcg cgtatccttg cgcttcacgc catcggcaag gggatgattt cggcctcgag
     9241 cgaggtagtg gagacgtgga tgtgcgccag cgtgacgggt agtcgaaacc ggcgtggacc
     9301 cgcgctgccc ggattgagat agagaatgcc cgcccgcttc tcgatcgatg gcttgtggga
     9361 atggccgcag acaatggcct tgaggccggc tgcaaccggg tcgacgctga gctgggtcgc
     9421 atcgtgcagc accaggatct tgtggttgcc ggcggacact accgcggttt cgggcaggca
     9481 ggaggcccag gcttggacgt cgatattgcc ccgcacggca acgacaggtg cgatttcgct
     9541 gaggcgctcg aggattgcgg gtttgccgac gtcaccggca tgaaggatca tcgcggacgg
     9601 acgcagcgcc gccagcgcct cgggccgcag cagaccatgg gtgtcagaga tgacgccgac
     9661 cagcatgaga tctcacctgg ccttcgccgc caagccatcc aggcgctcgg cgagcttctt
     9721 gagttgccag tcccgggcaa gcaaggcggc cttgccccat gtcggcttct gattgcgcag
     9781 cgggggtaat ggtgccgcct tgtccatggt cgcaatcgag cgatagagcc gaagcttatc
     9841 gccgagcgca gccaaccggc ccgcggcaag tgctttctcg agcgtgccat acttgcgcaa
     9901 tagcgaggcg gcgccgacgg cgccgatgcc ggctgctccg ggtatgcgat ccgatgcatc
     9961 gccgcgtagc gcgatgaagt ccggcacttg ctttggctcg accccgtagc gccgacgcac
    10021 ttcggcggga ccgatgcgcg ccatttcgcc ggcccggatc ggctgcagaa tggtggtatg
    10081 ctcggagacg agttgatagg cgtcgcgatc gccgctggcg accagcgccg tcccgccgcg
    10141 cttttcctcg cgggccactg ctgccgccag aaaatcatcg gcctcgtagc ccgctgcctt
    10201 ggcacaggca aagccgcagg ccgtcacgaa gccgggcagc acctcgagct gatcggtcag
    10261 ctcctcctcg aagctgcgcc ctgcttgata gctggcaagg gcctcatggc gataagtggg
    10321 ttcatcgagc gtatcccagc cgaccaggac cgcgcgcgga gcttccgtct gatagaggcg
    10381 caacaggaag ttggcaaagc ccagaatcgc ccccgcgccc ttgttgcccc gtcggcgtat
    10441 ggacttgggc aaggcgtggt aggccctgtg cgcgaacgaa tcgccatcga taatgaggag
    10501 cggtcgcctc atgcctcacc gtgccggcac cgcacttgac caaacgtgat gcgcatggcg
    10561 agccaagtgg tggtcggccg cgagccaaaa gcgccacgca ttccgtggat accgtcctcc
    10621 tggcgcagaa gagggggcta gggggtggtg gcgatcttca accgcgccca gtcctcgcga
    10681 aagccgatcc gccgcctcaa cctcgcctcg gcgcttttgc cttttcacgc gtcacc
//
LOCUS       DASTMT010000096         4270 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_4293471, whole genome shotgun sequence.
ACCESSION   DASTMT010000096 DASTMT010000000
VERSION     DASTMT010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4270)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4270)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4270
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4293471"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            219..1547
                     /locus_tag="VFR00_02000"
     CDS             219..1547
                     /locus_tag="VFR00_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011995192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEU0158051.1"
                     /translation="MAPLDMKPNELEPYWVPFTANRAFKKRPRLFAGAKGVHYFKPDG
                     SKVIDATSGLFCVNAGHCRDPIVKAIQDMAARLDYAPAFQYGHPKVFELASRLALLAP
                     GDLDYAFFSNSGSEAVDTALKVALGYHKAKGEGARTRFIGRERGYHGIGFGGISVGGI
                     VANRKQFGNMLTGVDHLPATYDRAKQAYSRGEPDWGAHLADELTRLINLHDASTIAAV
                     IVEPVSGSTGILAPPKGYLKRLRELTAQHGILLIFDEVITGFGRLGHCFAAERYGVVP
                     DMITFAKGVTNAAVPMAGVMASKQIYDAFMTGAEHMVELFHGYTYSGHPLAAAAGLAT
                     LDIYKDEGLFERARNLEAPFADAMMSLRGQPNVADIRTVGLLCGIDLDGVVGKPGLRG
                     FEATEHMFHELNLYVRVALDTLIVAPPLIASEADIGEIRDGIAKVLKAVA"
     gene            1719..3305
                     /locus_tag="VFR00_02005"
     CDS             1719..3305
                     /locus_tag="VFR00_02005"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEU0158052.1"
                     /translation="MAQQLGIPGSQSSPRRFADAFVIFSVTVLSMALGAWFLLQLGLS
                     LWPGMAAALSVYTVLLLFHLLVRRSLIDAGGEETREEMQWLAGEADPAAPATDRRDRS
                     SLAQPFAAEGEHSERLSQAFDDPHALPDPSTEVAFHYRPRDAGGALGSARVAARPPTN
                     PAPPRGAGEAWEGASSGSPSEKNVELIQDLIKKLADELSDVPATPATTAAPGGELEDA
                     MIARSLAALDTAAASMRAAAAEPSKEAAAGWWPTAQPPVGREADTSPARPLRSPAAAT
                     APPMPNAQVSRAAEAIAANRMETLLEPIQALNEGRARHFEISARFLAADGGAVETSAL
                     ERTGLIARMDAAGVVRAARVAQRLGERGREGNVLTRIAAESLTDPGFIDAVVQQAKHA
                     RGMGLILAVSQTAARTLTDLQISGLNALTTAGCRFALEDVGDLKMDFASLKALGFAFV
                     KLEAPVFLEGLAAAGGRVAASDVVRFLADYGLTLIVARIEDDWMLARILGFGVQFGMG
                     GLFGGPKLVKAEVVGEPAAA"
     gene            3427..>4270
                     /locus_tag="VFR00_02010"
     CDS             3427..>4270
                     /locus_tag="VFR00_02010"
                     /inference="COORDINATES: protein motif:HMM:TIGR01459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01459 family HAD-type hydrolase"
                     /protein_id="HEU0158053.1"
                     /translation="MSGTDIPILPSIRDLAGSAEAWIVDIWGVMHNGARAFEAAAEAC
                     AKFRQAGGSVLLLSNAPRPFTAVVSQLDGFGVARTAYDAGVTSGDVTRTMIAPWQGRP
                     VLHIGPARDRGLFDGFAIDFADAVAAEVIVCSGLFDDSTETPQDYAELFAGLVARRVP
                     MICANPDLVVERGDKLVYCAGALAADYAAIGGEVCYAGKPHWPVYERALAAIDALKGH
                     AVAKDKVLAIGDGIDTDLRGAHVAGLRSVFIASAVHTPEGLSPAVLQQLFRSRPFAPV
                     AALPA"
BASE COUNT          678 a         1426 c         1398 g          768 t
ORIGIN      
        1 tcggctaaca accgctaaat gccgatcaga ttcgggtgag tgtgtgccgg aatccgaacg
       61 aacacccggc aatgttaggg cggaccggag gcagcggcaa aaaactcttg ttttggcaag
      121 aggcgcaagc ccggcgggcc ggcggccgag cctatgcgcc aagcccggtc cctgctagaa
      181 cggccgcaac gtccaccttg caacccgcgg agactgccat ggccccgctc gacatgaagc
      241 ccaatgagct cgagccttat tgggtgccgt tcaccgccaa ccgcgccttc aaaaaacgcc
      301 cccgcctgtt cgccggcgcc aagggcgtgc attatttcaa gccggacggc agcaaggtca
      361 tcgatgccac ctcgggccta ttctgcgtca atgccggcca ttgccgcgat ccgatcgtga
      421 aagccatcca agacatggcg gcgcggctgg actacgcgcc cgcctttcaa tatggccatc
      481 ccaaggtgtt cgaacttgcc tcccgcttgg cgttgctggc gcccggcgac ctcgactatg
      541 cgtttttttc caactcgggc tcggaagcgg tcgacaccgc gctcaaggtc gcgctcggct
      601 atcacaaggc gaaaggcgag ggtgcgcgca cgcgcttcat cggccgcgag cgcggctacc
      661 acggcattgg ttttggcggc atctcggtcg gcggcatcgt cgccaaccgc aaacagttcg
      721 gcaacatgct gacgggggtc gaccatctgc ccgccaccta cgaccgtgcc aagcaggcct
      781 acagtcgcgg cgagcccgac tggggtgccc atctggccga tgagcttacc cgcctcatca
      841 atctgcacga tgcctccacc atcgccgccg tcatcgtcga gccggtctcg ggttccacgg
      901 gcatcctggc gcccccgaag ggctacctga agaggttgcg cgaactcacc gcccagcacg
      961 gcatcttgct gatcttcgac gaggtgatca ccggcttcgg gcgccttggg cattgcttcg
     1021 ccgccgagcg ctacggcgtc gtgccggaca tgatcacctt tgccaagggc gtcaccaacg
     1081 ccgccgtgcc gatggccggt gtgatggcct caaagcagat ctacgacgcc ttcatgaccg
     1141 gggccgagca catggtcgag ctgttccacg gctacaccta ctcgggccac ccgttggcgg
     1201 cggcggccgg cctcgccacc ctcgacatct acaaggacga gggactgttt gagcgggcac
     1261 gcaacctcga ggcgccgttt gccgatgcca tgatgagctt gcgcggccag cccaacgttg
     1321 ccgacatccg cacggtcggt ctcctgtgcg gcatcgatct cgatggggtc gtgggcaagc
     1381 ctgggctgcg cggctttgag gcaacggagc atatgttcca cgagctgaac ctttacgtgc
     1441 gtgtcgccct cgatactctg atcgttgccc cgcctctgat cgccagcgaa gccgacatcg
     1501 gcgagattcg cgatggtatc gccaaggtgc tgaaggcggt cgcctagcac ggccgcttgg
     1561 caatactggg cgccctcggg gccacccgcg ccgacgcggg tggccggccc tggagagctt
     1621 gaaagctggt gatagtccgc cccagccggt tgcgcatgca gccgcggcgc aggaatgctt
     1681 tggccagcac gaatcaaaaa acagcggagt tcggctgcat ggcacagcaa ctcggcattc
     1741 caggctcgca gtcctctcct cggcgcttcg ccgacgcctt tgtcatcttc tcggtgacgg
     1801 tgctgtcgat ggcgctcggc gcctggttcc tcctgcaact cggtttgtcg ctgtggcccg
     1861 gcatggcggc cgccctcagc gtctatacgg tgctgttgct gtttcatctg ctggtgcgcc
     1921 gatccctgat cgatgccggc ggcgaggaga cacgcgagga gatgcaatgg cttgccggcg
     1981 aggccgaccc tgccgcgccg gcgactgatc ggcgcgacag gtcgtctctg gcgcaaccct
     2041 tcgccgccga aggcgagcac tccgaacgct tgtcccaagc tttcgatgat cctcacgcgc
     2101 tgcccgatcc gagcaccgaa gtggccttcc attatcggcc gcgggacgcc ggcggggctt
     2161 tgggcagcgc ccgggtggcc gcgcggccgc ccaccaatcc cgcgcctcct cgtggtgccg
     2221 gcgaggcctg ggagggcgca tcgtctggct caccctcgga aaagaacgtt gagctcattc
     2281 aggatctcat caagaagctg gccgacgagc tgagcgacgt gcccgcaacg ccagcgacga
     2341 cggcggcgcc cggcggggaa ctcgaggacg ccatgatcgc ccgctcgctg gcggcgctcg
     2401 atacagcggc cgcctccatg cgcgctgccg cagctgagcc ctccaaggag gcggccgctg
     2461 gctggtggcc gacggcgcag ccgccggttg gacgcgaggc ggacacatcc cctgcgcggc
     2521 cgctgcgctc gccggccgcg gcaacggcgc caccaatgcc caacgcccag gtttcacgtg
     2581 ccgccgaagc catcgccgcc aatcgcatgg aaaccctgct tgagccgatc caggccttaa
     2641 acgagggccg ggcgcgtcac ttcgagatca gcgcccgctt tttggccgcc gatggcggag
     2701 cggtcgagac cagcgcgctg gagcgcaccg ggctcattgc acgcatggat gccgccgggg
     2761 tcgtacgcgc tgcgcgcgtg gcccagcggc tgggcgaacg cggccgcgag ggcaacgtct
     2821 tgacgcgcat tgccgccgaa tcgctgaccg atccgggctt catcgatgcc gttgtgcaac
     2881 aggccaaaca cgcgcgtggg atggggctga ttctggccgt gagccagacc gccgcgcgca
     2941 cgttgacgga cctccaaatc agcggcttga acgcgttgac gacggcgggc tgccgctttg
     3001 cgctcgagga cgttggcgat ctgaagatgg acttcgccag cctcaaagcg ctgggatttg
     3061 cctttgtgaa gctcgaagcg cccgtattcc tggaaggatt ggctgcggcc ggcgggcggg
     3121 ttgcggcctc cgatgtggta cgcttccttg ccgattatgg gctgaccttg atcgttgcac
     3181 gcatcgagga cgattggatg ctggcgcgca ttctgggctt tggcgtgcag ttcggcatgg
     3241 gcgggttgtt cggtgggccg aaactggtaa aggccgaggt cgttggggaa ccggctgccg
     3301 cttgattgct tgcgcgcctg ccgctagagg atgctagcac gcccccggcc ctcgggggcg
     3361 tcgactgggt tgatcgcaca cgaggtaccg tgcagtcctt gccgccggtc ctgctctagg
     3421 gcgcacatga gcgggacgga cattcccatt ctaccttcca tccgtgacct cgccggcagc
     3481 gccgaggcgt ggattgttga tatttggggc gtcatgcaca atggtgcgcg cgccttcgag
     3541 gcggcggcag aagcctgcgc caagtttcgc caagctggcg gcagtgtgct gttgctgtcg
     3601 aacgcgccgc gacccttcac ggccgtggtg tcccagctcg acggttttgg cgtggcgcgc
     3661 acggcctatg atgcgggcgt aacctcgggc gatgtgacgc gcacgatgat tgcgccctgg
     3721 caaggacgcc ccgtcttgca cattggccct gcgcgcgacc gcggcctgtt tgatggtttt
     3781 gccatcgatt ttgccgacgc cgtagccgcc gaagtgatcg tctgctccgg actgttcgac
     3841 gacagcaccg agacgccgca agactatgcc gagctgtttg cgggcctggt tgcgcggcgt
     3901 gtgccgatga tttgcgcaaa ccccgatctc gtggtcgagc gcggcgacaa attggtctac
     3961 tgcgcgggag cccttgccgc cgactatgcc gcgatcggcg gcgaggtctg ctatgcgggg
     4021 aaaccgcatt ggcccgtcta tgaacgcgcg ctggcggcga tcgatgcgct caagggccat
     4081 gcggttgcca aggacaaggt gctggcgatc ggcgacggca tcgacaccga cctacgcggc
     4141 gcccacgtcg ccggtctgcg ttcggtcttc atcgccagcg ccgttcatac ccccgagggt
     4201 ttgagccccg ccgtcctgca gcagttgttc cgctcccgtc ccttcgcacc ggtggccgca
     4261 ctccccgccc
//
LOCUS       DASTMT010000097         3468 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_52309, whole genome shotgun sequence.
ACCESSION   DASTMT010000097 DASTMT010000000
VERSION     DASTMT010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3468)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3468)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3468
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52309"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            184..258
                     /locus_tag="VFR00_02015"
     tRNA            184..258
                     /locus_tag="VFR00_02015"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:216..218,aa:Gly,seq:gcc)
     gene            930..1976
                     /locus_tag="VFR00_02020"
     CDS             930..1976
                     /locus_tag="VFR00_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014892920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HEU0158054.1"
                     /translation="MSEPPRALHRAQTHDVLRAARDWLDADGRIALATVIGTWGSAPV
                     GIGGQMVVAQDGRFEGSVSGGCVEGEVIAEAEDILASGEPKTLDFGVADETAWQVGLP
                     CGGQIRVYVERLEKDDGLPLIERALAARDGRRGLLVRTHLKDGRHEIFARDDQGLDPS
                     VRKRFESGESELEMTPEGEIFLHAMVPPARVLIIGATHIGQILAQLVTMAGYEVMVID
                     PRTAFAAASRFPDIRLDTDWPQDAIPKIGLDPYTAVVALAHVGHIDDEALKLAMRSNC
                     FYVGALGSNRNHAKRKQRLTEAGFNDQEIGRIHCPIGFNIGAQTPQEIAIAIMAELVL
                     ALRGPKEYKPARVS"
     gene            2052..3254
                     /locus_tag="VFR00_02025"
     CDS             2052..3254
                     /locus_tag="VFR00_02025"
                     /EC_number="2.3.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012654362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoadipyl-CoA thiolase"
                     /protein_id="HEU0158055.1"
                     /translation="MLDAYIYDGLRTAFGRHAGALSRVRPDDLLAGVVKETVARAKFA
                     PEFIDDVSVGCASQAGEDSRNVARHAILLSGLPVTTPGSVHNRLCGSGLNALVAAAQA
                     ITCGEAQVCLAGGVESMTRAPFVVGKAEQAFSKAFAVYDSSLGARFPNPKLEKEFGAD
                     SMPQTADNLARDYQLSREACDRFALASQLKYATANAAGFFKDEIHPVAIPQGKGKADL
                     VVAADEHPRPDTSFESLQKLKPLFSDGVTTAGNASGINDGAAALIVASRAAGERAGVK
                     PIARILASAVAGVPPRVMGFGPVPAIQKALERAGLQLKDIDVIEINEAFAAQVLACLK
                     GLGLAFEDPRVNPNGGAIAIGHPLGASGARLALTATRQLVRSEARYALVSMCIGVGQG
                     IAVVLERA"
BASE COUNT          547 a         1173 c         1177 g          571 t
ORIGIN      
        1 cgcgcaaaca ggtcctgacg gctggcaggc acgatgcttc gatcctccac aggcgcgaca
       61 ggccccgcgc cttgtgcttc ctagccgatg gcgaacgccg ggcacaatgg ccgcatgggg
      121 cccacgccag ggcgcctgcg cttgccaacc ctggggcttt gctgcatata cccgcacgtg
      181 gatgcgggtg tagctcaggg gtagagcatc acgttgccaa cgtgagggtc gtgggttcaa
      241 atcccatcgc ccgctccaat ctcggtttgt cgcaggcggt cccgtggccc ggacctgcgg
      301 cggccggccg ggccgccgcc gcccagcgcc gtcgccccca acgaagccag cattgccatc
      361 gcgcgtgtac cggggacatg ggtgacgcct ggcgcaccgc gttgccgtca gcggcgcctt
      421 ggccgcggtc caagcccacg cttttgtcgc aagccggccg ctgcgcgtgc ccggcggcgg
      481 cttgcggcgc tgggtccggc gcggggtgtt gtcgcacaag gagcctcggt ggcccggccg
      541 cgccgccggc cgccggcact ttggcgccgc cgcgcgtgcg gcaagggcgc cgcagggccg
      601 ggtcggacca agcctgttgc cgtgaggcgc aatcccctcg cggggcttac ttttcttgcc
      661 ggcaagcctt aacgccttgc ctttgtctgg gcgccgagcc cgccggcgcg gcccgcctcg
      721 tcgacgccag accttcgctg gccgcgcggt ccgggcgacc ggccagcgcc cttgcgccag
      781 cgtcacgtga ccctcgcagg ccagcgtcgc gaatgtcggt actggcatcg gcggccgccc
      841 ccgcttattt tccgctatgg ccgccggccg atcgggcggc gcccgccgtt gctgccagag
      901 cagcccattt tcaggcaaga ggctacacca tgtccgaacc cccacgcgcg ctgcataggg
      961 cgcaaaccca cgacgtcctg cgggcggcac gcgattggct cgacgcggat ggtcgcattg
     1021 ccctggcgac ggtgatcggc acgtggggat cggcgccggt cggcattggc gggcagatgg
     1081 tggtggctca ggatgggcgc ttcgagggct cggtctccgg cggctgtgtc gagggcgagg
     1141 tgatcgccga agcggaggat attctcgcca gcggcgagcc caagaccctc gatttcggcg
     1201 tcgcggacga gaccgcctgg caggtgggct tgccgtgcgg cgggcaaatc cgcgtctatg
     1261 tcgagcgcct ggagaaagat gacggcttgc ccctgatcga gcgcgctctc gcggcacgcg
     1321 acggccgccg cggcctgttg gtgcgcacgc acctcaaaga cggccgccac gagatctttg
     1381 cgcgcgatga tcagggtctc gatcccagcg ttcgcaagcg ctttgaaagt ggcgagagcg
     1441 aacttgaaat gacgcccgag ggggaaatct ttttgcacgc catggtgccg cccgcgcgcg
     1501 tgctcattat cggggccacg cacatcggcc agatcctggc gcagctcgtc accatggcag
     1561 gctatgaggt gatggtgatc gatccgcgca ccgcctttgc cgccgcaagc cgcttccccg
     1621 acattcgcct cgataccgat tggccgcagg acgcgatccc gaagattggg cttgatccct
     1681 atacggccgt cgtggccctc gcccacgtcg ggcatatcga cgatgaggcg ctgaagcttg
     1741 ccatgcgctc caactgtttc tacgtcggcg cgctcggctc caaccgcaac cacgccaagc
     1801 gaaagcagcg cctcacggag gcgggcttca acgaccagga gatcggccgc atccactgtc
     1861 cgatcggctt caatatcggc gcccagacgc cgcaggagat cgccatcgcc atcatggctg
     1921 agctggtgct ggccttgcgt ggtcccaagg aatacaagcc ggctcgggtg agctaaggct
     1981 acggcggggt cgcgccgtgc cgaagcgggc gaccaggtcc cccctgtagc ggccatggag
     2041 gaacaggcat catgctcgac gcttacatct acgacggtct gcgcacggcc ttcggccgcc
     2101 acgccggtgc actttcgcgc gtgcgtccgg atgacctgct cgcaggcgtc gtcaaagaga
     2161 ccgtggcgcg cgcaaaattt gctccggagt tcatcgatga tgtctccgtc ggctgcgcca
     2221 gccaagccgg tgaggacagc cgcaacgttg cccggcacgc gatcctgcta tcgggattgc
     2281 cggtgacgac gcctggatcg gtgcacaatc gtctgtgtgg ttcagggctc aatgccctgg
     2341 tggcggcggc gcaggcgatc acctgcgggg aggcgcaggt gtgtctggcc ggcggcgtgg
     2401 aaagcatgac gcgcgcgcca ttcgtggtgg gcaaggcgga gcaagccttc agcaaggcct
     2461 tcgccgtcta tgactcgtcg ctgggcgccc gttttcccaa tccgaagctg gaaaaggagt
     2521 tcggcgccga cagcatgcca cagacggcgg acaatctggc tcgcgactat cagctttcgc
     2581 gcgaagcctg cgaccgcttt gcgcttgcct cccagcttaa gtacgccacg gctaatgcgg
     2641 ctggattttt caaggacgag atccatccgg ttgccatccc ccagggcaag ggcaaagccg
     2701 atctcgtggt cgcggcggat gagcatcccc gtcccgatac ctcctttgag agcctgcaaa
     2761 aactcaagcc cctgttcagc gacggcgtta cgaccgcggg caacgcctcg ggcatcaacg
     2821 acggagcggc cgctctcatt gtcgccagtc gggcggccgg cgagcgggcc ggcgtcaagc
     2881 cgatcgcgcg cattcttgcc tccgccgtcg ccggcgtgcc gccgcgcgtg atgggcttcg
     2941 gcccggtgcc ggcgatccag aaggcgctgg agcgcgcagg cctgcaactc aaggacatcg
     3001 atgtcatcga aatcaacgaa gcatttgccg cgcaggtgct ggcctgcctc aaggggcttg
     3061 gcctcgcctt cgaggacccg cgcgtcaatc cgaacggtgg cgcgatcgcc atcggccatc
     3121 cgctcggcgc ctcgggcgct cgtctcgcgc tgaccgcgac gcggcaactg gtgcgctccg
     3181 aggcgcgcta tgccttagtc tccatgtgca tcggcgtggg ccagggcatc gccgtggtcc
     3241 tcgaacgcgc ctgagacgcg cgaaccggcg ccggcgcgac cgcggcggcg caccaatccg
     3301 gtggcacctc agcgcgcgag cccgtgcctc gacgcaggtg caatattgca ccgccgaaat
     3361 gacgggctgt tcataacagc acattttggt agcgtacccg ccgcggaggg ggattgaaga
     3421 cggtaacgtg ggaaggggag cgaaacgaac gaccaaggag gccgggcg
//
LOCUS       DASTMT010000098        14355 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_2677017, whole genome shotgun sequence.
ACCESSION   DASTMT010000098 DASTMT010000000
VERSION     DASTMT010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 14355)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14355)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14355
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2677017"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(42..923)
                     /locus_tag="VFR00_02030"
     CDS             complement(42..923)
                     /locus_tag="VFR00_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011995303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU0158056.1"
                     /translation="MSAPAPGPTSRIYFSQRLRLHYVDWGNAGAPPLVLVHGGRDHCR
                     NWDWVAEELRQDWHVIAPDLRGHGDSQWSQDGTYTMAGYIYDLAQLIHQLKFGPVSII
                     AHSLGGNIALRYSGLYPENVSKLIAIEGLGPSPKLLRERQAKPFAERMRTWIAEQRAL
                     SGRVARRYASIEHAYRRMQEENRHLSPQQARHLTVQGVNQNEDGTYSWKFDNYVRSWP
                     PYDMRSKDIEALWGRIACPTLLLYGKESWASNPAEDGRVAAFKQAKVATIEKAGHWVH
                     HDRLDEFLSLTRAFLAR"
     gene            complement(1078..1371)
                     /locus_tag="VFR00_02035"
     CDS             complement(1078..1371)
                     /locus_tag="VFR00_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYD domain-containing protein"
                     /protein_id="HEU0158057.1"
                     /translation="MANYILLASYSEQGLKGIKETVKRTEAVRELAKKAGITMQESYW
                     TLGQYDVVAIFEAPDDESMTAFALSIAKLGNVKTQTLRAFSAKEMTSILGKMV"
     gene            complement(1469..1876)
                     /locus_tag="VFR00_02040"
     CDS             complement(1469..1876)
                     /locus_tag="VFR00_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009492265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEU0158058.1"
                     /translation="MAQKIVRSVKSMVEAAKREIEELPAGEAVKLFGREDVVLVDLRD
                     PRERERDGKVPGTFNVTRGMLEFWIDPQSPYYKDTFGQDKKFVFFCAGGLRSALAAKT
                     AQDMGLKPVAHVLGGFKGWKEAGGPVEPGTPKT"
     gene            2011..2469
                     /locus_tag="VFR00_02045"
     CDS             2011..2469
                     /locus_tag="VFR00_02045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0158059.1"
                     /translation="MPVRYTLPAVALLFGACAAALPGYTPPPLKEKKVAKLTGQTTSG
                     EMTGEGTYELSELDKARDCKRTLGSMQISIARLKDPAFRAEPSAASSGMQSGSASLYG
                     GSARGADRQSEYARERARLDAYNRLLASKNCKTLDIDAELAKPPEEVKRY"
     gene            complement(2507..3838)
                     /locus_tag="VFR00_02050"
     CDS             complement(2507..3838)
                     /locus_tag="VFR00_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0158060.1"
                     /translation="MSRMSHRAPTSLAVAVAAVLMGSALPAQAQTAQRDGIGELIGRK
                     PEAQAEQGAPKPNAGPAATGQAAEPAKDPREGEASYEQAQRLMKAVDAILQDTARQRG
                     EAKKLPPEADFLVKPIWTETREDREKKVRELLDAALGIVTDVPVVDVQKKIEGLRKNI
                     RELDDRTVKLREKQLLAPKDGMLPGYLTDTVDSLGKDIEDTKKRIDLNREEIKQAKSE
                     VLEALKKAGIQLSPEQVDLLLDSVLSGDLVRLVAVFNSAKVIDLQLGKLMTASGENIG
                     AARKYFAMHAALFALLVHAQDGLIAKIDNQYLPKLSAIERDIRAARVKTAELLKAENR
                     DDQKRALEGNRESQKLAEEAAKGYRRYLLQQREQVAKARQRATHDLRIADNTYETVEA
                     SFQLKILMQDSAASFEAIQKLEAPTFDQIFRNEELRREFESLTRKLDAPSS"
     gene            complement(3926..4684)
                     /locus_tag="VFR00_02055"
     CDS             complement(3926..4684)
                     /locus_tag="VFR00_02055"
                     /inference="COORDINATES: protein motif:HMM:NF039496.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5655 domain-containing protein"
                     /protein_id="HEU0158061.1"
                     /translation="MPIAIGTESGYDSGFPGWGALSNARRGTNTMAADERQEEQRFLA
                     ELKARSGRDLADWMALIAAQNFSDKNETIDWLRAQGFAFARASWLERIHSNGGRPIYQ
                     EAAERPPRPPPRPRAEKGAAAPAPLPPQPDPGPGEAAQLEALIAQAKGYRPLYLMLET
                     EVKRALPGLVLRPAATHISFAAPAEFAAVTLAASEVRLGLALGEHAFDARLQRAKLRG
                     AGEHITHMVVLTDARQVNADLISVILAANARING"
     gene            complement(4727..7198)
                     /locus_tag="VFR00_02060"
     CDS             complement(4727..7198)
                     /locus_tag="VFR00_02060"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HEU0158062.1"
                     /translation="MQRVLIWIAAVCALLVLLVALAVGGAWAWLRATVPSTAGSLVLE
                     GLSAPVAITRDREYVPHIFASSRHDLLFALGFAHAQDRLWQMELSRRSGQGRLSEIFG
                     ERTFTTDVFLRTLDLYGHAERSLAALSPEDRADLDAYARGVNGFMTRRTGWFEPRFAP
                     EFILLRHTPEPWRPADSMVLAKLMALQLGANINHELARLAYAAHGLKAAEIEDLMPTE
                     AADHPPPLPELAELYPLQRTAGGGRRAAAGVEGAAIGEGASNNWVVAGSRTRSGKALL
                     ANDPHLGLTAPSVWYLAHLALEQPGVAPVNVVGASLAGAPLIVLGRTDSIAWGYTNTG
                     TDVQDIFIEKINPDNSAEYLTPEGWRPFERESMAIAVKGSGVRAVERRRTRHGPVLPG
                     FYRGLETLLAPGHVAALQWTALSDDDTTLAVGLLEPGLKTVADYMARMQRYVGPMQNM
                     VVADLDGHIGLIAPGRVPRRDAANKVAGRAPVPGWDARYDWKGYVPFQELPRLLDPPQ
                     GAIGTANARILPPNSPVHLTFDWDPAFRQRRIDELIVAPERGPHDIGSMRQAQADVLS
                     LAVTRLQPLMIAAAQIGETVDQQLLDQLTGWDGTMRADRPEPLIFMAWLRAAVRAIYG
                     DDLGPAFERFWGARASALIRLLEGRATGRDWCDDQSTPTRESCGAVLAAALARAIDDL
                     ERRYGKDRSQWRWGNAHRALSEHRPLGTFGKLAGLVDVAGFVNISVASPGDDYTLDVG
                     RMDFASAEPFANRHAASYRAIYDFADLDRSLYMHSTGQSGNPFSPFYRSFVERWAGVD
                     YIEIATKRDRIAALGTWALTPRSAK"
     gene            complement(7273..7716)
                     /locus_tag="VFR00_02065"
     CDS             complement(7273..7716)
                     /locus_tag="VFR00_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU0158063.1"
                     /translation="MSIAVRRLEAKDKSVWLRLFKGYIEFYKATVAEAVIEETWRRLL
                     AGGSDFHIGLVAVDASDTPIGLAHVLFHRSTWSPTQYCYLEDLFVDPARRKSGAGRAL
                     IEATYREADARGATRTYWTTQEFNYTARALYDQVATKSVFVQYRR"
     gene            complement(7810..9177)
                     /gene="mgtE"
                     /locus_tag="VFR00_02070"
     CDS             complement(7810..9177)
                     /gene="mgtE"
                     /locus_tag="VFR00_02070"
                     /inference="COORDINATES: protein motif:HMM:TIGR00400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HEU0158064.1"
                     /translation="MQDETPGERAIEPMSELAPAIAEALAAHDEAHIRSSLANLQAPD
                     LADLIEFLEPPQRVALVETLGAAFDPEALSELDEGVRDQLLADLPQATVAKAVAELET
                     DDAAYVLENLDATQRQEILALIPSIDRAALERNLEYPEETAGRLMQTDFVAVPPFWTV
                     GQVIDHMREADDLPDTFSDIYVIDPTYHVVGSVDLSRLLRTKREVPVETIMDGARQVV
                     LATADQEEVARQFERYDLKSAPVVDANRRLVGVVTVDDVVEVIQEETEEDLRRLGGVG
                     DESVMDTVIDTVRNRFAWLLINLATALLASSVIKLFDATIGQMVALAVLMPVVASMGG
                     NAATQTMTVTVRALATRDLGPANTLRVIVREASVGFINGVAFAAIVGLIVLFWFGVGT
                     LGLVIATAMICNFLAAALAGIMIPLTLARFGYDPAVASGVFVTTVTDVVGFFAFLGLA
                     SLWLR"
     gene            9497..10468
                     /locus_tag="VFR00_02075"
     CDS             9497..10468
                     /locus_tag="VFR00_02075"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEU0158065.1"
                     /translation="MRVSGAWGLLFCVLAGAPALAAPPTCGPQALGVARTLEIDTRQA
                     PRFGFQYPDTGLLADGEVILTFDDGPLRAYTRPILEALAAHCTKATFFVVGRMAVADP
                     EMVQEYARQGHTVGTHTWSHANLQAMSPARARAEVELGFSAVQAAMGRPIAPFFRFPY
                     LRDTKWASDYLTSRQLAAFSIDVDSKDYRTRDPSAVHRKVMADLARQKKGIILFHDIH
                     YSTARALPSLLTDLKAKGYKVVHVTPKEDVVTVAEFDGQAQQEMERRRATLAGEPLAR
                     RALTWPAAKTVPSQPAAIELPTTTPPVRASEKNGNSWTSQVHWWPWR"
     gene            10630..11832
                     /locus_tag="VFR00_02080"
     CDS             10630..11832
                     /locus_tag="VFR00_02080"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU0158066.1"
                     /translation="MLRSAFYAFAASLAISVATGAPLAAQEAPKSIKVGYVISLSGPQ
                     SAGAMLTTVPNYRLWVDEVNKAGGLMLKKWNKRIPIEVMEIDDRSNNEDMVRLVERLM
                     TVDKVDIVLSPWGTGPNLQAAPVFAKNGYPQLVGTGNSDKIPELVQRFPTTFWFLGMP
                     SDGAKALVDLLSDLKGAGQINDKVALFVVQHPFGSEFVTPTKPLLEKAGFKLAYETTY
                     PLGVSDLSAQIKAAKAAAPDTLVAFSYPPDTFMLTEQSIANDFSPKVKFLSVGVAFPN
                     YKAKFADKVEGTFGLGAWNPDGPGVKDWFARHKSLTNQEPDRWASSNTYASLQILQQA
                     IEQVGEIDRPKIVEAIKTGTFKTILGDTKLEAGVLRKVWWVGQWQDGEYRGIAPTDLP
                     GAAKPRLK"
     gene            11995..13956
                     /locus_tag="VFR00_02085"
     CDS             11995..13956
                     /locus_tag="VFR00_02085"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0158067.1"
                     /translation="MLLFDFLLAGLVIGGIYALISIGLNLQYGVARVLNLAYGEILML
                     AGYGAFWGFTLIAAPPLLTLIVAAPLAFGLSWLLFRYLLHPLLKRARDPGKREIDSIL
                     ATFGLLFVLQGGALVAWSGTDRFYSFLAQPVNVLGVVMSANRLLVLVAALLLSAATFA
                     LLQLTAIGRALRALAASPASAPLVGIDVARYSAGAFALGGALAAIAGVLLSSFMGINP
                     TIGAAFTMKALIVVVMGGIGHVLGGLVAGLVLGMAETLAALLIDPGLITVINFAIFSL
                     VLLWRPQGLFARNAGTGRANPSRWSRHLAGAALLGLLLLAPRFANAYWLSLLVNILMY
                     VALATGWAFFSGATRYVSLAASAFFGVGAYAVAVFAPDYSYPVALLAALGISSLLALF
                     VGLATLRLSGMYFVIFTFGLATLVREIVTWWEFNIAQKAGRHLFLSITTTHIYYQLLA
                     LCAGIIALWLLRDRSRLGFALTTLGADETVARQIGINTVALKVGTFVVSALTMTLAGA
                     ILAPRWSYMDATVAFNPNTSFLTVIMALLGGATSVWGPMLGVVPLVLISDYLSVTVPN
                     YFSVVLGLVLLAIVFLVPNGLIGLSEPLSRWAAREHMAQLLRRLAGRLDAGAGAPKTD
                     DALPLPAQPRLLPPARRRRWLGAGREGEA"
     gene            13953..>14355
                     /locus_tag="VFR00_02090"
     CDS             13953..>14355
                     /locus_tag="VFR00_02090"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU0158068.1"
                     /translation="MTRAQPSLLAVSQLSRNFGGLAALDGVSFEVARGRIFGLIGPNG
                     SGKTTLLNIVCGRLEPTRGEVRIAGQPMTGRRPEVMCRAGIAGTFQLARVPDALDALD
                     NVAVAAMYGRRRLSVAAARAEAERLLARVGYI"
BASE COUNT         2353 a         4852 c         4596 g         2554 t
ORIGIN      
        1 ggcccgtccg cggcaagcgc tcgtcggcct cgacggcggc gctaacgggc aagaaaggcg
       61 cgcgtcaggc tcaagaactc atcgagccgg tcgtggtgca cccaatggcc agccttttcg
      121 atcgttgcca ccttggcctg cttaaaggcc gccacccgcc cgtcctcggc cgggttggag
      181 gcccaactct ccttgccgta gagcagcaag gtcgggcagg cgatgcggcc ccacagggct
      241 tcgatatcct tgctgcgcat gtcgtagggc ggccaggagc gcacgtaatt gtcgaacttc
      301 cagctgtagg tgccatcctc gttctggttg acgccttgca ccgtcaagtg gcgcgcttgt
      361 tgcggtgaga ggtgcctgtt ctcctcctgc atgcgccgat aggcatgctc gatggaggcg
      421 tagcggcgcg cgacgcggcc cgagagcgcg cgctgctcgg cgatccaggt gcgcatgcgc
      481 tccgcaaagg gcttggcttg gcgttcccgc agcaacttcg ggcttggccc aagcccctcg
      541 atggcgatca gtttgctcac gttttcgggg taaagcccgc tgtagcggag cgcgatattg
      601 ccgcccagcg aatgcgcaat gatgctgacc gggccgaact tcagctgatg aatgagttgc
      661 gccaggtcgt agatgtagcc ggccatcgtg taggtgccgt cctgcgacca ttggctatcg
      721 ccgtggccgc gcaggtcagg ggcaataaca tgccaatcct ggcgcagctc ctcggccacc
      781 cagtcccaat tgcgacaatg atcgcgcccg ccatgcacca acaccagggg tggcgcgccc
      841 gcgttccccc agtcgacgta atggaggcgc aggcgttgag aaaaatagat gcgggaggtc
      901 gggccgggag caggtgcgga catggccaaa actaggactt tccgtttgtg cagtgcaaca
      961 ggagcttagc cccgccgcgc ctccggcgga agcgcggcgc catgccccgt cccagggcgc
     1021 ggcggcagac gcgaccagcg ccgccgccgg gacccctcaa agacgatttt ggcggcctca
     1081 caccatcttg ccgagaatgg aggtcatctc cttggcggag aaggcgcgca gcgtttgcgt
     1141 tttgacgttg ccgagtttgg caatcgaaag cgcaaaggcc gtcatgcttt catcgtcggg
     1201 agcctcgaaa atcgccacca cgtcgtattg gcccagcgtc cagtagctct cctgcatggt
     1261 gatgccggcc tttttggcca gctcccgcac cgcctcggtg cgcttcaccg tttccttgat
     1321 gcccttcagt ccctgttcgc tataactcgc aagcaagatg tagtttgcca tgacggcttc
     1381 ctccgaccga cgccgcccgt cgttgccgcg cgcgagccta atccgcccgc atccggaggg
     1441 gaatggcaaa gcgggtgccg tgggtggctc acgtcttggg cgtgccgggc tcgaccggcc
     1501 cacccgcctc cttccagcct ttgaagccgc ccagcacgtg cgccacgggc ttcagcccca
     1561 tgtcctgcgc cgttttggcg gccaaggccg agcgcaaccc gccggcgcag aaaaacacga
     1621 acttcttgtc ctggccgaag gtgtccttgt agtaggggct ttgcggatcg atccagaatt
     1681 ccagcatgcc gcgcgtcaca ttgaaggtgc cgggcacctt gccgtcgcgc tcgcgctcac
     1741 gcgggtcgcg cagatcgacc agcaccacgt cctcgcggcc aaacagtttg accgcctcgc
     1801 cggccggaag ctcttcgatt tcgcgcttgg cggcctccac catggatttg acgctgcgga
     1861 caattttctg tgccatcgta cgtctccggc ttattcgagc tgaagggggc catgtgcggc
     1921 ctgcgtggcc ctctccttac ggcagacgcc ctttcaccgc cacgtttaga tggctagcgt
     1981 agcagcgaat ctcggtaaga cctggggccg atgcccgtgc gctataccct tcctgccgtc
     2041 gccctgctgt ttggcgcctg cgcggcagca ttgccgggct ataccccgcc tcccctcaag
     2101 gaaaaaaagg tggccaagct caccggccag acgacgagcg gggagatgac gggggagggt
     2161 acctatgaac tcagtgagct cgacaaggcg cgcgactgca agcgcaccct gggctcgatg
     2221 cagatcagca tcgctcggct caaggatccg gccttccgcg ccgagccgtc cgccgcctcc
     2281 tcgggcatgc aaagcggcag cgcctcgctt tatggcggct cggcgcgtgg cgcggaccgg
     2341 cagagcgagt acgcgcgcga gcgcgcccgg ctcgacgcct ataaccgcct gcttgccagc
     2401 aaaaactgca agaccctcga catcgatgcg gagctcgcca aaccgcccga ggaggtcaag
     2461 cgctactgag gacgccgtcg accggcgcct cgcaacggct tggcgtctag ctcgagggcg
     2521 catccagctt gcgcgttaag ctctcgaact cgcgccgcag ttcctcatta cgaaagatct
     2581 gatcgaaggt cggggcctcg agcttttgga tggcctcgaa ggaggccgcc gaatcctgca
     2641 tcaggatctt gagctgaaag ctcgcctcca cggtttcgta ggtgttgtcg gcgatacgca
     2701 agtcgtgcgt tgcgcgctgg cgcgccttgg caacctgctc gcgctgctgc agcaggtagc
     2761 gccggtagcc tttggccgcc tcttcggcca gcttttggct ctcgcggttg ccctcgagcg
     2821 cgcgcttctg gtcgtcgcgg ttctccgcct tgagcaactc ggcggttttg acgcgcgcgg
     2881 cgcgaatgtc gcgctcgatc gcgctcagct tgggcaggta ctgattatcg atcttggcga
     2941 tcaaaccatc ttgggcgtgc accaggagcg caaacagagc cgcgtgcatg gcaaaatact
     3001 tgcgcgccgc cccgatattc tcgcccgacg ccgtcattag tttgccgagt tgcaggtcga
     3061 tcaccttggc ggaattgaag acggcgacca ggcgcacgag atcgccggac agcacgctgt
     3121 ccagcaacag gtcgacctgt tcggggctca actggatgcc ggcctttttc agggcctcca
     3181 gcacttcaga tttggcctgc ttgatctcct cgcggttgag atcaatgcgc ttcttggtgt
     3241 cctcgatatc cttgcccagg ctgtcgacgg tatcggtcag atagcccggc agcatgccat
     3301 ccttgggcgc gagcaactgc ttctcgcgca gtttgacggt gcgatcgtca agctcgcgga
     3361 tgttcttgcg caaaccctcg atcttctttt gcacgtcgac cacgggcacg tcggtgacga
     3421 taccgagcgc cgcgtccaac agctcgcgca ccttcttctc gcggtcctcc cgcgtctcgg
     3481 tccagatcgg cttgaccagg aaatcggctt cagggggaag cttcttggcc tcgccccgct
     3541 ggcgcgcggt atcctgcagg atggcgtcga ccgccttcat caggcgctgc gcctgctcgt
     3601 agctggcctc accctcgcgc ggatccttgg ccggctcggc ggcttggccc gtcgcggcgg
     3661 ggccggcatt gggcttcggc gcgccctgct ccgcttgggc ctcgggcttg cgcccgatca
     3721 actcgccaat cccgtcgcgc tgggccgttt gtgcctgggc cgggagcgcg gatcccatca
     3781 gcacggcggc aacggcaacg gctaagctcg tcggcgctcg atggctcatt ctcgacatga
     3841 ccccgcacct cctaatgtgg caagggtgtc caagcaaatc gactttttcg tggttggcta
     3901 atcactttag ccgttgccgc gggcatcagc cgttgatgcg ggcattggct gccaagatca
     3961 cgctgataag atcagcgttc acttggcggg catcggtcag cacgaccatg tgcgtgatat
     4021 gttcgcccgc acccctcagt ttggcgcgtt gcaagcgcgc atcgaaggcg tgctcgccca
     4081 gcgccaggcc gagccgtacc tcgctggcgg cgagggtgac ggcagcaaac tccgccggcg
     4141 cggcaaacga gatgtgcgtt gctgccggac gcaggacgag acctggcagc gcccgcttca
     4201 cctccgtctc gagcatcagg tagagcggtc ggtaaccctt ggcttgcgcg atcaacgcct
     4261 cgagctgggc cgcctcgcca ggtccagggt ctggctgcgg cggtagtggc gcgggcgcgg
     4321 cggcaccttt ttcggcgcgc ggccgcggcg gggggcgcgg cgggcgctcc gccgcctcct
     4381 gatagatcgg ccgcccaccg ttgctgtgga tgcgttcgag ccacgacgcg cgcgcaaagg
     4441 caaaaccttg cgcgcgcagc caatcgatgg tctcgttctt gtcgctgaag ttttgcgcgg
     4501 cgatgagcgc catccagtcg gccaaatcgc gccccgagcg ggccttcagc tccgccaaga
     4561 accgctgttc ctcctggcgc tcgtctgccg ccatggtgtt ggtgcctctg cgggcattgc
     4621 tcagcgcacc ccagcctggg aagcccgagt catagcccga ttcagtaccg atcgcgatcg
     4681 gcattgcccc gcgcggcgcg cgccgcaatc tccttggcgc gcctggctac ttggcactcc
     4741 taggcgtcag cgcccaggtg ccaagcgcgg cgatgcgatc gcgcttggtc gcaatctcaa
     4801 tatagtcaac gcctgcccaa cgttcgacga acgagcggta gaagggagag aacggattgc
     4861 cggactggcc ggtgctatgc atgtaaagcg agcggtcgag atcggcaaaa tcatagatgg
     4921 cgcggtagct cgcggcatgc cgattggcaa agggttccgc gcttgcgaag tccatgcgac
     4981 cgacgtcaag ggtgtaatcg tcaccggggc ttgccacgct gatgttgacg aagccggcaa
     5041 cgtccaccag cccggctagc ttgccgaacg tacccagcgg ccggtgctcg ctgagcgccc
     5101 gatgcgcgtt gccccaacgc cattgcgaac gatccttgcc gtagcgccgt tctaaatcgt
     5161 cgatcgcgcg cgccagcgcg gcggcgagca cggcaccaca gctttcccgc gtcggggtgc
     5221 tctggtcgtc gcaccaatcg cgacccgtcg cgcgcccctc cagcaaacgg atcaacgcgc
     5281 ttgcccgtgc accccagaag cgctcgaagg ctggccccaa gtcgtcgcca tagatggctc
     5341 gcacggccgc gcgcagccag gccatgaaga tcaacggttc cggccgatcg gcgcgcatcg
     5401 tgccatccca acccgtcagc tggtcgagga gttgctggtc gaccgtttcg ccgatttgcg
     5461 cggcggcgat catcaagggc tgcaggcgcg tgaccgcgag cgaaagcacg tcggcttggg
     5521 cctggcgcat gctgccgatg tcgtgcggtc cgcgctccgg cgcaacaatc agctcatcga
     5581 tgcgccgctg ccggaaggcc ggatcccaat cgaaggtcag atgcaccgga gaattgggag
     5641 gcaggatgcg cgcattggcg gtgccgatgg cgccctgcgg cggatcgagc aggcgcggca
     5701 gctcttggaa tggcacgtag cccttccaat cgtaacgtgc atcccagccg ggcaccggcg
     5761 cgcggccggc gaccttgttg gccgcgtcgc gccgaggcac gcggcccggc gcgatcaaac
     5821 cgatgtgccc gtcgagatcg gcgaccacca tgttctgcat gggcccgacg tagcgctgca
     5881 tgcgggccat gtaatccgcc accgtcttga ggccaggctc gagcaggccg acggcaagcg
     5941 tggtgtcgtc atcgctcaag gccgtccatt gcaaagccgc gacgtgcccc ggcgccaaca
     6001 gcgtctcgag cccgcgatag aagccgggca gcacgggccc atgccgcgtg cgtcggcgct
     6061 cgaccgcacg cacaccagag cccttgacgg caatcgccat actctcgcgc tcaaacgggc
     6121 gccaaccctc cggcgtcaag tattccgcgc tgttgtcggg attgatcttc tcgatgaaga
     6181 tatcctgcac atcggtgccg gtgttcgtgt acccccaggc gatgctgtcg gtgcgcccga
     6241 gcacaatcag cggcgctccg gcaaggctcg ccccgaccac gttgaccggc gccacgcccg
     6301 gctgctccag ggcgagatgc gccaggtacc acaccgaggg cgccgtcagg ccgaggtgcg
     6361 gatcgttggc gagcagcgct ttgccggagc gcgtgcgcga gccggccacc acccaattgt
     6421 ttgaggcccc ctccccgatc gccgcgccct caacgccggc cgcggcgcgg cgcccgccgc
     6481 ccgcggtgcg ttgcagagga tagagctcgg cgagctccgg cagcggcggg ggatgatcgg
     6541 ccgcttctgt gggcatcagg tcttcgatct ctgccgcctt caggccgtgc gcggcatagg
     6601 cgagacgggc cagctcgtgg ttgatattgg cgccgagttg cagcgccatc agtttggcaa
     6661 gcaccatgct gtcggccggc cgccagggct ccggggtatg ccgcaagagg atgaactccg
     6721 gggcaaagcg cggctcgaac cagccggtgc ggcgcgtcat gaagccgttg acgccgcggg
     6781 cataggcgtc gaggtccgcc cggtcctcgg ggctcaaggc cgccagcgag cgttcggcgt
     6841 gcccgtaaag atcgagcgtg cgcaggaaga cgtcggtcgt aaaggtacgc tcgccgaaga
     6901 tttcagagag gcggccttgg ccgctgcggc gcgacaactc catctgccac agccgatcct
     6961 gggcatgggc aaagccgagg gcaaacaaga gatcgtggcg ggagctcgca aaaatgtggg
     7021 ggacatactc gcggtcacgc gtgattgcca cgggcgcgct gagcccctca agcaccaggc
     7081 tgcccgccgt ggagggcacc gtggcgcgca gccaggccca tgccccaccg accgccaagg
     7141 ccaccaacag gacgagcagc gcgcaaaccg ctgcgatcca gatcaggacg cgctgcatcc
     7201 gcgagcccta ccctgcaaaa cccgcttcct tggactcatg cgccgcccgt tgcccgccat
     7261 tgagcgctcg cgctagcgcc gatactgcac gaacaccgac ttggttgcga cctggtcgta
     7321 gagcgcgcgt gcggtgtagt tgaactcctg cgtcgtccag taggtgcgcg tggcgccgcg
     7381 ggcatcggct tcgcgatagg tcgcctcgat caacgcgcgt ccagcgccgc ttttgcgtcg
     7441 cgccgggtcg acgaacaggt cctcaagata gcaatactgg gttggcgacc aggtcgagcg
     7501 gtgaaacagc acgtgcgcca acccaatcgg cgtatcgctg gcgtcgacgg ccaccaggcc
     7561 gatgtggaag tcggagccgc cggccagcaa gcggcgccag gtctcctcga tgaccgcctc
     7621 ggccacggtg gccttgtaga attcgatgta gcccttgaag agccggagcc aaaccgattt
     7681 gtccttcgcc tcaaggcgcc gcacggcgat gctcatggcc acaactctct aacctgctcg
     7741 cttgatggtt tttcgctact cacgcgtgcc aactctggca catcggctgg gcgcgaacca
     7801 gctccccggc tatcgcaacc aaagcgaggc gaggccgagg aaggcaaaga aaccgacaac
     7861 gtcggtgacc gtcgtcacga acacgcccga ggccacagcg gggtcgtagc caaaacgcgc
     7921 gagcgtcaag ggaatcataa tgccggcgag cgccgccgcc aggaaattgc aaatcatggc
     7981 ggtcgcaatc accagcccga gcgtgccgac gccgaaccag aacaggacga tgagcccgac
     8041 gatggcggcg aacgccacac cgttgatgaa cccaaccgag gcctcgcgca cgatcacacg
     8101 cagcgtgttg gccgggccca ggtcgcgggt ggcaagcgcg cgtaccgtca ccgtcatggt
     8161 ctgggtcgcg gcattgccac ccatcgaggc aaccacgggc atcaacaccg ccagcgccac
     8221 catttgcccg atggtggcgt cgaataattt gatgacactg gaggcgagca gcgcggtcgc
     8281 gaggttgatc agcagccaag caaagcggtt gcggacggtg tcgattaccg tgtccatcac
     8341 gctttcatcg ccgacgccac cgagccgccg caggtcctcc tcggtctcct cctggatcac
     8401 ctcgacgacg tcatcgacgg tgacgacgcc gaccagccgc cggttggcgt ccaccaccgg
     8461 cgccgatttc aggtcgtagc gctcgaactg gcgcgccacc tcttcctgat cggccgtggc
     8521 gagcaccacc tggcgggcgc catccatgat ggtttccacc ggcacctcgc gcttggtgcg
     8581 cagcagccgg ctcaagtcga cgctgccgac cacatgatag gtcgggtcga tcacgtagat
     8641 atcggaaaag gtgtccggca gatcgtccgc ctcgcgcatg tggtcgatca cctggccgac
     8701 ggtccagaac ggcggcacgg ccacgaaatc ggtctgcatg agacggccgg ccgtctcttc
     8761 ggggtattcc agattgcgct cgagcgcggc gcggtcgatg ctggggatca gcgccaggat
     8821 ctcctggcgc tgggtcgcat ccagattttc caggacatag gcggcatcgt ccgtctcgag
     8881 ctcggccacc gctttggcca cggtcgcctg cggcagatcg gccagcagct ggtcgcgcac
     8941 gccctcgtcc agctcggaga gggcctccgg gtcgaaggcg gccccgaggg tctcgacaag
     9001 ggcaacgcgt tgcggcggtt ccaggaactc gatcagatcg gcaaggtcgg gcgcctgcag
     9061 gtttgccagc gaggagcgga tgtgagcctc gtcgtgggcc gcgagcgcct cggcgattgc
     9121 cggcgcgagc tcggacatcg gctcgatggc gcgctcgccc ggggtctcat cttgcatggc
     9181 gcggatccct gcgtcggccg ccacgtccgc ggcgaccatc gggcgattgg gccccgctgg
     9241 ggaggcccaa aagtgtggcc gctcaagggg gcgccggcgt ccggcctccc ccttgggcag
     9301 gcccatcctg gcgcatacca cactctgatg ctctagcgac acagcccgcg cgcaaaacgt
     9361 tggtgcaaaa aagcaaactc ggccgaggca tttatcggca agcggcggcc ttaccttgcg
     9421 tataccccgc gcgcatgtca gaagccgccc ttgaggcgga ttacggcggg ccgatcggac
     9481 gagggaagct ggcaagatgc gcgtttctgg cgcgtggggg ttgcttttct gcgtgttggc
     9541 cggcgcaccg gcgctggcag caccgccaac ctgcggtccg caggcgctgg gcgtagcgcg
     9601 cacgctcgag atcgatacgc ggcaagcccc gcgcttcggc tttcaatatc cggacaccgg
     9661 cttgctggcc gatggcgagg tgatcctcac cttcgatgat ggcccgttgc gcgcctacac
     9721 cagaccaatc ctggaggcgc tggccgccca ctgcaccaag gccaccttct tcgtggtcgg
     9781 gcgcatggcg gtcgccgacc ccgagatggt gcaggaatac gcgcgccagg ggcatacggt
     9841 cggcacccac acctggtcgc acgccaacct gcaggccatg tcaccggcgc gcgcacgcgc
     9901 cgaagtcgag ctcggtttca gtgccgtgca ggccgcaatg ggtcgaccga tcgcgccttt
     9961 cttccgcttt ccctatctgc gcgacaccaa gtgggccagc gactatctga cgagccggca
    10021 gctggcggcc ttttccatcg acgtcgacag caaggactat cgcacccgcg atccctcggc
    10081 ggtgcatcgc aaggtgatgg ccgacctggc gcgccagaag aagggcatca tcctgttcca
    10141 cgatatccac tactcgaccg cgcgcgcctt gccgagcttg ctcaccgacc tcaaggccaa
    10201 gggctacaag gtcgtgcacg tcacccccaa ggaggacgtg gtcacggttg ccgaattcga
    10261 cggccaggcg cagcaggaaa tggaacgccg gcgtgcgacg cttgccggcg agccgctggc
    10321 gcgacgcgcc ttgacttggc cggcggccaa gaccgttccg agccagcccg ccgcgatcga
    10381 gctgccgacg acaacgccgc ccgtgcgcgc cagcgagaag aacggcaact cctggaccag
    10441 ccaagtgcac tggtggccct ggcgctgagg acgacccctt aacagctgat ttttgcgcgc
    10501 gctaccgcca gccgcggcgg cgcttggcac gttggcgcgg gctcggctgc gccagggcaa
    10561 ccttgactag ttggaggtcc aactaatagg ttggtgcaaa gactaataac agcgataggg
    10621 aggcccagta tgttgcgatc agcgttttat gcttttgccg cgagcctagc gatcagcgtt
    10681 gccaccggcg ctccgctcgc cgcgcaagag gcgccaaagt ccatcaaagt cggctatgtc
    10741 atttcgctgt cgggaccgca gtcggccggg gcgatgctga cgacggtacc gaactatcga
    10801 ctgtgggtcg acgaggtcaa caaggccggc ggcctgatgc tgaaaaaatg gaacaagcgc
    10861 attcccatcg aggtgatgga gatcgacgac cgttccaaca acgaagacat ggtgcgcctg
    10921 gtcgagcgtc tgatgaccgt cgacaaggtc gatatcgtgc tctcgccgtg ggggacgggc
    10981 cccaacctgc aggccgcacc cgtattcgcc aagaacggct atccgcagct ggtcggcacc
    11041 ggcaactccg acaaaatccc cgaactcgtg cagcgcttcc cgaccacctt ctggttcctc
    11101 ggcatgccca gcgatggcgc caaggcgttg gtcgatctcc tatccgacct caagggggcc
    11161 ggccaaatca acgacaaggt cgccttgttc gtggtgcagc atcccttcgg ttcggagttc
    11221 gtcaccccga ccaaaccgtt gctcgaaaag gcgggcttca agctcgccta cgagaccacc
    11281 tatccgctcg gggtgtccga tctgtccgcc cagatcaagg cggccaaggc ggcggcgccc
    11341 gacacgctcg tcgccttctc ctatccgccc gacaccttca tgctgaccga gcagtcaatc
    11401 gccaacgatt tcagcccgaa ggtcaaattc ctgagcgttg gggtcgcctt tcccaactac
    11461 aaggccaaat tcgccgacaa ggtcgaaggc acctttggtc tcggcgcctg gaaccccgac
    11521 ggccccggcg tcaaggactg gttcgcccgg cacaaaagcc tgaccaacca ggagccggat
    11581 cgctgggcca gctccaacac ctacgcctcc ctgcagatct tgcagcaggc catcgagcag
    11641 gtcggcgaga tcgatcggcc caagatcgtc gaggcgatca agaccgggac cttcaaaacc
    11701 attcttggcg acaccaagct cgaagccggc gtgctgcgca aagtctggtg ggtcggccag
    11761 tggcaggatg gggagtatcg cggcatcgcc cccaccgacc tgccgggcgc cgccaagccg
    11821 cgcttgaagt agcctataca tggcttaacg cccccgcggt cccgcgttca tgacgcggga
    11881 cttttttccc gcaggccttg cacccgaacc cctataaccg aggggcgcgg caggctcggg
    11941 ggcctgccgc ggcacgcgtc ccccgagcaa ccacgaaccc gcaaacccca ggcaatgctc
    12001 ctcttcgatt tcctgctggc aggtctggtg atcggcggca tttatgcgct gatctcgatc
    12061 ggcctcaact tgcagtatgg cgtcgcgcgc gtgttgaacc tcgcctatgg cgagatcctg
    12121 atgctggccg gctacggcgc cttctgggga tttacactta tcgccgcccc gccgctcttg
    12181 accctcatcg tggcggcgcc gctggccttc ggcctgagct ggctgctctt ccgctacctg
    12241 ctgcaccccc tgctcaaacg tgcgcgcgac ccgggaaaac gcgaaatcga ttctatcctc
    12301 gccaccttcg ggctcttgtt cgtgctgcag ggcggcgcgc tggtggcttg gtccggcacc
    12361 gaccgcttct acagttttct cgcccaaccg gtgaatgtgc tgggcgttgt catgagcgcc
    12421 aatcgcctgc tggtgctggt ggccgcgctc ctgctgtccg cggcaacctt cgcgcttttg
    12481 cagctgaccg cgatcgggcg ggccttgcgg gcgctggcgg caagccccgc gagcgcgccc
    12541 ctggtcggca tcgacgttgc ccgctacagc gcgggggcgt ttgcgctggg gggcgcgctg
    12601 gccgccatcg ccggcgtgct gttgtcgagc ttcatgggca tcaatccgac catcggtgcc
    12661 gccttcacca tgaaggcgct gatcgtcgtg gtgatgggcg gcatcggcca cgtgctcggc
    12721 ggcctggtgg ccggcttggt gctcggcatg gccgagacct tggccgcgct cctgatcgat
    12781 cccggcctca tcaccgtcat caactttgcc atcttctccc tcgtgctgtt gtggcggcca
    12841 caaggcctgt ttgcccgcaa tgccggcacc ggccgcgcca acccttcgcg ctggtcgcgt
    12901 cacctcgccg gcgccgcgct gctcggcttg ctgctgctgg cgcccagatt tgccaatgcc
    12961 tattggctct cgttgctggt caatattctg atgtacgtgg cgctggcgac gggctgggcg
    13021 ttcttttccg gcgcgacacg ctacgtctcg cttgccgcgt cggccttctt cggcgttggc
    13081 gcctacgcgg tggccgtctt cgcccccgac tattcctacc ccgtggcgct gctggccgcg
    13141 ctcggcatct cgagcctgct cgccctattc gtcggcctgg caacgctgcg cctgtcgggt
    13201 atgtacttcg tgatcttcac cttcggcctc gccacgctgg tgcgcgaaat cgtgacctgg
    13261 tgggagttca acattgccca gaaggcgggg cgacacctgt tcctgtcgat caccaccacg
    13321 cacatttact accagctatt ggccctgtgc gccggcatca tcgccctgtg gctgctccgc
    13381 gatcggagcc ggctcggctt cgccttgacc acgctggggg ccgacgaaac ggtggcgcgc
    13441 cagatcggca tcaacacggt ggccctgaag gtcggcacct tcgtggtgag cgcgctcaca
    13501 atgacgctgg ccggggccat tctcgccccg cgttggagct acatggatgc caccgtcgcc
    13561 ttcaatccca acacctcgtt cctgaccgtg atcatggcgc tcttgggcgg cgccacctcg
    13621 gtgtgggggc cgatgctggg cgtcgttccg ctggtgctga tctccgatta tctgtcggtg
    13681 acggtgccca actatttcag cgtggttttg gggctggtgc tgctcgccat tgtcttcctg
    13741 gttcccaacg gcctaatcgg cttatccgag ccgctcagcc gctgggccgc acgcgagcac
    13801 atggcccagc tgttgcgccg cctcgcgggc cgcctcgatg ccggcgccgg cgcgcccaag
    13861 accgacgacg cgctccccct gcctgcccag cctcggcttt tgccaccggc gcgccgacgg
    13921 cgctggctcg gcgcggggcg ggagggcgaa gcatgacgcg cgcgcaaccc tcgctgctcg
    13981 cggtgagcca actcagccgc aactttggtg ggctggccgc gctcgatggc gtttccttcg
    14041 aggtggcgcg cgggcggatt tttggcctga tcggacccaa cggctcgggc aagaccaccc
    14101 tcctcaacat cgtctgcggt cgcctggagc cgacgcgggg agaggtacgt atcgccggcc
    14161 agccgatgac gggccgccgc cccgaagtca tgtgccgcgc ggggatcgct ggcaccttcc
    14221 aactcgcgcg cgtgcccgat gcgctcgatg cgctcgacaa cgtcgccgtc gcagccatgt
    14281 acggccggcg ccggctcagc gtggcggcgg cgcgcgcgga ggccgagcgt ttgctggcgc
    14341 gcgtgggcta tataa
//
LOCUS       DASTMT010000099         1642 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_2348778, whole genome shotgun sequence.
ACCESSION   DASTMT010000099 DASTMT010000000
VERSION     DASTMT010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 1642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1642
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2348778"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..536)
                     /locus_tag="VFR00_02095"
     CDS             complement(<1..536)
                     /locus_tag="VFR00_02095"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU0158069.1"
                     /translation="MQSLTGKVAWVTGAGTGIGEAAALALANAGMTVVLTGRRAAMLE
                     AVAERIKAAGRGTAQVRAADLTNSQAVAAVADGLTGQFGRLDVVVNNAGTNLPNRTWR
                     QLTPGAIATLIEGNLTSAFYVVAAALPIMRAQGDGLFIHTASWAGRFIGPVSGPGYTA
                     AKHGMVAMSHTINLEECM"
     gene            complement(611..1564)
                     /locus_tag="VFR00_02100"
     CDS             complement(611..1564)
                     /locus_tag="VFR00_02100"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEU0158070.1"
                     /translation="MTVRIGYLLPTRERIMEGRADTEALLALAERAARLGYDSIWAGD
                     SLLARPRHEPLTLLAAVAGRVPGVTIGTAVLLPALRNPVLLAHQVATIDQIAEGRLIL
                     GVGIASDVANIRGEFAAAGVPFEKRVGRMLEGLRLCRALWTGQPVDWDGRWRVERGIL
                     APLPHRPGGPPIWLAGALPASLQRVGQHFDGWLPNSPTAARWAEQWQEVAAVARAAGR
                     DPKALSGAMYLTMAVDEDAARAQARLDAYLAQYYGRPAAEMRERQACYAGPAQGLAAW
                     LRGYVAAGARHLVLRFAGDHERHLETIAGFSAGLRAGEPAA"
BASE COUNT          267 a          602 c          530 g          243 t
ORIGIN      
        1 tgcatgcact cctcgagatt gatggtgtgg ctcatcgcca ccatgccgtg cttggcggcc
       61 gtatagccgg ggccggacac cggaccgatg aaccgcccgg cccatgaagc ggtatggatg
      121 aaaaggccgt cgccctgcgc acgcatgatg ggcagcgctg cggcaacgac atagaacgcg
      181 ctcgtcaaat tgccctcgat gagggtggca atggcgcctg gggtcagctg ccgccaggtg
      241 cgattgggaa ggttcgtccc ggcattgttg acgacgacat cgagcctgcc gaactgaccg
      301 gtcagaccat cggcgaccgc cgcgacagcc tgggagttgg tgagatcggc agctctcacc
      361 tgcgccgtgc cgcgtccggc agccttgatc cgctccgcga cggcttcgag cattgccgcg
      421 cggcggccgg tgagaaccac cgtcatgccc gcattggcca gcgccagcgc ggcggcctcg
      481 ccgatgcccg tgcctgctcc cgtcacccac gccaccttgc ccgttagcga ctgcatggcc
      541 gatgtctcct tccagttgcc gcctcgtcta gcctcgccca aacaaccccg atagtcggcg
      601 ggccgagcgg ctaggccgcc ggctcgccgg cgcgcagccc ggcgctgaag ccggctatgg
      661 tctcgaggtg gcgctcgtga tcaccggcaa agcgcaagac cagatgcctc gcccccgccg
      721 ccacgtagcc tctgagccat gccgccagtc cctgggccgg ccctgcataa caggcttggc
      781 gttcccgcat ttcagcggcc gggcgcccgt agtattgggc aagataggcg tcgagccgcg
      841 cctgcgcgcg tgcggcatcc tcgtcgacgg ccatggtcag atacatggcc ccgctcagcg
      901 ccttgggatc gcgtccggcg gcgcgagcca cggccgcaac ctcctgccac tgctcggccc
      961 agcgtgcggc cgtgggcgag ttcggtagcc agccgtcgaa atgctggccg acccgctgca
     1021 agctggccgg taacgcccct gccagccaga tcggcggtcc gcccggccga tgcggcaacg
     1081 gcgccaggat gccccgctcg acccgccaac ggccgtccca atcgacgggc tggccggtcc
     1141 acagcgcccg acagagccgc agcccctcca acatgcgacc gacgcgcttt tcaaacggca
     1201 cgccggcagc cgcaaattcg ccgcgaatat tggcgacgtc gctggcaatg ccgacgccca
     1261 ggatcaggcg gccttcggca atctgatcga tggtggcaac ctggtgggcc aagagcaccg
     1321 ggttgcgcag cgccggcagc agcaccgccg tgccgatcgt gacgccgggc acccgtccgg
     1381 ccaccgcggc gagcaacgtc aagggttcat gtcgcggcct ggccagcagc gagtcacccg
     1441 cccagatcga atcatagcca agccgcgcgg cccgctcggc aagcgcgagc aaggcctcgg
     1501 tgtccgcacg accctccatg atccgctcgc gggtcggtag cagatagccg atgcgcaccg
     1561 tcatggggat accttctcat ctgctggcaa acccggcgcc ggcacctctt cgggtcagcc
     1621 cgagcgacat tgccaccgta gc
//
LOCUS       DASTMT010000100         1832 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_709194, whole genome shotgun sequence.
ACCESSION   DASTMT010000100 DASTMT010000000
VERSION     DASTMT010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 1832)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1832)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1832
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_709194"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..789
                     /locus_tag="VFR00_02105"
     CDS             <1..789
                     /locus_tag="VFR00_02105"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009764363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEU0158071.1"
                     /translation="DLTLTENAQPALMAASLAVLRVLEVEKGFRLADRVKFVAGHSLG
                     EYSALAAAGALSLADAARLLKVRGKAMQAAVPVGQGAMAALLGVGKDVAERLAAEAAQ
                     GEVCQLANDNEPTQAVISGNKAAIERAGGLAKAHGVRRFLPLNVSAPFHCALMQPAAD
                     AMAEALAGVDVKPPSVPLVANVLAAPISDAAEIKARLIEQVTATVRWRESVAAMAAAG
                     VSAVYEIGAGKVLAGLAKRIAPSLNASSIGTPPELEAALPALLT"
     gene            799..1542
                     /gene="fabG"
                     /locus_tag="VFR00_02110"
     CDS             799..1542
                     /gene="fabG"
                     /locus_tag="VFR00_02110"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006610958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HEU0158072.1"
                     /translation="MFELVDKTALVTGATGGIGGAIARALHRQGATVAISGRQLDRLE
                     ALAAELGSRVVVCPCDLGRRDEVAKLIDTAIAKLGRLDILVNNAGLTKDNLFMVMKDE
                     QWDEVIAVNLTSTFMLMRTAARAMMRAKTGYGRIINISSVSGLIGNPGQGNYAAAKAG
                     MIGMSKSLAREVASRGITVNCIAPGFITTPMTEALNEKQTAGIKEAIPAQKFGSPDDV
                     AGAAVYLASLEAAYVTGATLHVNGGLVMV"
     gene            complement(1614..>1832)
                     /locus_tag="VFR00_02115"
     CDS             complement(1614..>1832)
                     /locus_tag="VFR00_02115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0158073.1"
                     /translation="ASTPRCSTTIFFTRAATSLIALSLGSLLIHAVAVCQKSGLVKPT
                     PETAPWLEQIRGPTADISWVERLNQRAR"
BASE COUNT          329 a          592 c          619 g          292 t
ORIGIN      
        1 gacctgacgc tgacggagaa tgcccagccc gccctgatgg cggcttccct cgccgtgctg
       61 cgtgtcctcg aggtcgaaaa aggcttccgc cttgccgatc gggtcaagtt cgtggccggt
      121 cactcgttgg gggaatactc ggccctggcg gcggccggcg ccttgagcct ggccgacgcc
      181 gcccggcttt tgaaggtgcg gggtaaggcc atgcaggccg cggtgccggt agggcagggg
      241 gcgatggcgg cgctgttggg ggtcggcaag gacgtggccg agaggctcgc cgccgaagcg
      301 gcgcaaggcg aggtttgcca actcgccaac gacaacgaac caacgcaggc cgtgatctcg
      361 ggcaacaagg cggcaatcga gcgggcgggc ggtctcgcca aggcgcatgg cgtgcgccgc
      421 ttcctgccgc tcaacgtcag cgcccccttc cattgtgccc tgatgcagcc ggccgccgac
      481 gccatggctg aggccctggc cggcgtcgac gtcaagccgc catcggtgcc cctggtggcc
      541 aacgtgctgg cggccccaat cagtgatgcc gctgagatca aggcgcgcct catcgagcag
      601 gtcaccgcca ccgtgcgctg gcgcgaaagc gtggcggcca tggcggcggc cggcgtcagc
      661 gcggtctacg agatcggcgc cggcaaggtg ctcgcgggac tggccaagcg cattgcgccc
      721 agcctcaacg ccagctcgat cggcacgcca ccggagctgg aagccgccct gccagccctc
      781 ctgacatagg aaaaattcat gttcgaactg gtggacaaaa cggcgcttgt cacaggtgcg
      841 acgggcggca tcggcggcgc catcgccagg gccctgcaca ggcagggcgc aacggtggcc
      901 atctccgggc gccagctcga ccggctcgag gcgctcgccg ccgagctcgg cagccgcgtc
      961 gtggtctgcc cctgcgacct tggccgccgg gacgaggtcg ccaagctcat cgatacggca
     1021 attgccaagc tcgggcggct cgatatcttg gttaataacg ccggcctgac caaggacaac
     1081 ctgttcatgg tcatgaagga cgagcagtgg gacgaggtga tcgccgtcaa cctgacctcg
     1141 accttcatgc tgatgcggac ggcggcgcgg gccatgatgc gcgccaagac cggctacggc
     1201 cgcatcatca acatttcctc cgtcagcggc ctcatcggca accccggcca gggcaactat
     1261 gccgccgcca aggccggcat gatcgggatg tcgaaatcgc tggcacgcga ggtggcctcg
     1321 cgcggaatta cggtcaattg cattgccccg ggctttatta ccacccccat gaccgaagcc
     1381 ctcaacgaaa agcagaccgc cggcattaag gaagccatcc cagcccagaa gttcggcagc
     1441 ccggacgatg tggccggcgc ggccgtctac cttgcctccc tcgaggccgc ctatgtaacc
     1501 ggcgcgaccc tgcatgtgaa cggtggcttg gtcatggttt gacggcgatt ttgccggcga
     1561 ttattgaccc tgggatgcgc ccgaggaatg ctggtcaagg ccaatgaagt gtgttaacga
     1621 gcccgctgat ttaggcgttc aacccaggaa atgtctgctg tcgggccgcg aatctgctcg
     1681 agccatggcg cagtttcggg cgtgggcttg accaggcccg atttttggca cacggcaaca
     1741 gcgtggatca gcaaagagcc aagggataga gcaatgagcg acgtcgcagc gcgcgtgaag
     1801 aaaatcgtgg tggagcacct cggcgtcgag gc
//
LOCUS       DASTMT010000101         4002 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_1038448, whole genome shotgun sequence.
ACCESSION   DASTMT010000101 DASTMT010000000
VERSION     DASTMT010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4002)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4002)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4002
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1038448"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..184
                     /locus_tag="VFR00_02120"
     CDS             <1..184
                     /locus_tag="VFR00_02120"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEU0158074.1"
                     /translation="TECTGGAAKITEADLARQYDTGCDPRLNAAQSLELAFRLAERLQ
                     AEKATAEQRRAPRAAE"
     gene            complement(252..1106)
                     /locus_tag="VFR00_02125"
     CDS             complement(252..1106)
                     /locus_tag="VFR00_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013167527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEU0158075.1"
                     /translation="MQSSTSATLLGSGAILLWSSLASLTVLKGAVPPLQTTAIAFAVG
                     GLVLLAAAAVRGRLPYLVPTRASLALGIYGLFCYHALYFAALSLAPAAEASLIASLWA
                     LFTVVFSGLLPGQPLRLRHLVAALIGLAAATLLVWNSLGVDSTASHRPLGFLLAFGCA
                     LIWSTYSVASRLFAAVPSDSIAVPCLITAALAGALSLALETWVSPGASSWLALVGLGI
                     GPVGAAFLLWDVGMKAGDVSLLGVLSYAAPVLSTGLLVVLGLAPPTLQLGLACALMVI
                     ATAIAARS"
     gene            1251..2930
                     /locus_tag="VFR00_02130"
     CDS             1251..2930
                     /locus_tag="VFR00_02130"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013947837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HEU0158076.1"
                     /translation="MSAPSHTLKIALAQLNPVVGDLEGNAAKLVAARQDAAAKGADLI
                     VLPELFITGYPPEDLVRKPAFARAARAAIEGLAARTADAGPGVLVGSIWPEDGKLYNA
                     AALLDRGKVAGVRFKVDLPNYGVFDEKRVFDVGPMPGPLNFRGVRIGVPICEDIWQEE
                     ICECLQETGAELLISPNGSPFDWTKPDLRMNVAVARVTENALPLIYVNQVGGQDELVF
                     DGASFVLNADRALAVQLAAWEECVGLITLNRSAQGWNAERQPLSLLEEHEAAAYHACV
                     LGLRDYVDKNRFPGVVMGLSGGVDSALVAAMAVDALGAQRVHCIMLPYRYTSNESLSD
                     AAACAEALGVRYDTIPIAPAVEGFGAMLEAQFAGRAKDITEENVQSRVRGTTLMALSN
                     KFGSLVLTTGNKSEMSVGYATLYGDMNGGFNPIKDLYKTEVYRLCAWRNRHHPKGGKG
                     PSVPVIPENILNKAPTAELRENQKDQDSLPPYEVLDDILACLVEQELGLADIVARGHA
                     PAIVKKVEHLLYLAEYKRRQAAPGVKISARNFGRDRRYPITNKFREKLGES"
     gene            complement(3160..3825)
                     /locus_tag="VFR00_02135"
     CDS             complement(3160..3825)
                     /locus_tag="VFR00_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002713998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEU0158077.1"
                     /translation="MADAAAVPRTNATRERILELAEAAVLDKGFAATSIEELIAGAGI
                     TKSGFFYHFRDKGELARALMLRYIAHNDAVLDDIFVRADELNEDPLHGFLVALKLFAE
                     MMAELPATHPGCLVASFCYQDRLFDGEVRTLTQTGVRSWRKLFLDRLEIIAGRYPPKV
                     ALSLEDLADMANTLVDGGIIMSRVLVDKTILPRQILLYREFIRTVFLGPASERTPGSG
                     AQS"
BASE COUNT          769 a         1248 c         1365 g          620 t
ORIGIN      
        1 caccgagtgc acgggcgggg cggccaagat cacggaggca gacctggccc gccagtacga
       61 taccggctgt gacccgcgcc tcaacgccgc ccagtccctg gagctggctt tccggctggc
      121 cgagcggctg caggccgaga aggcaacggc agagcagcga cgcgcacccc gggccgcgga
      181 ataggcggca agcagcgcgg cccgcgacca caaggttggc gcagcagcgc ggcggtcagc
      241 gaccgccgcg ctcaggagcg tgccgcaatc gccgtggcga tcaccatcag cgcgcaggcg
      301 agcccgagct gcagggtggg cggggccaag cccagcacca ccaatagccc ggtcgagagc
      361 accggtgcgg cgtaggacag cacgcccagc agcgagacat cgccggcctt catgccgacg
      421 tcccagagca aaaacgcggc ccccaccggg ccgatgccca gaccgacgag cgccaaccag
      481 gaggacgcgc cgggcgaaac ccaggtctca agggcgaggc tcaaggcgcc cgccagggcg
      541 gccgtgatca agcagggcac ggcgatgctg tcactgggca cggcggcaaa caggcgcgag
      601 gccaccgagt aggtggacca aatcagggcg caaccgaagg ccaagaggaa gcccagggga
      661 cgatggctcg ccgtgctgtc aacgcccagg ctgttccata ccaggagtgt tgcggccgcg
      721 agcccgatca aggcggcgac gagatgcctg aggcgcagtg gttgtcccgg caacagtccc
      781 gagaacacca ccgtgaacag ggcccagagc gaggcgatca ggctggcttc ggccgcgggg
      841 gccaagctca gcgccgcgaa atagagcgcg tggtagcaaa agaggccgta gatgccgagg
      901 gcgagcgagg cgcgggtcgg cacgaggtac ggcaggcggc cgcgcaccgc tgccgcggcg
      961 aggagaacga ggccgccgac cgcgaatgcg atcgccgtcg tctgcaatgg cggcacagcg
     1021 cccttgagca cagtcaagct ggcaagcgaa ctccagagca ggattgcgcc cgagcccaac
     1081 agcgtggcgg aggtggagga ttgcatcggc tcggcagcgc ccttttgcgt gtggcgcgca
     1141 acttgcccca acttgggcgc tgggcgccag ccccgagcgc gagcctgggc gaggcagggc
     1201 gcgccgggct cgattgcaag ggagggccgc ccggcctaca tcttcaaggc atgtccgctc
     1261 cgagccatac cctgaagatc gccttggccc agctcaatcc cgtggtcggt gacctcgagg
     1321 gcaacgctgc caaactcgtg gccgcacgcc aggacgccgc cgccaagggc gcggatctca
     1381 tcgtcttgcc ggagctcttc atcaccggct atccgcccga agacctggtg cgcaagccgg
     1441 cctttgcgcg tgcggcgcgc gccgcgattg agggcttggc ggccagaacc gccgatgctg
     1501 ggcccggcgt cctcgtcggc agcatctggc ccgaggatgg caagctctac aacgcggcgg
     1561 ctttgttgga tcgcggcaag gtcgccggcg tgcgcttcaa ggtggacctg cccaactatg
     1621 gggtattcga cgaaaaacgc gtattcgacg tgggacccat gcctggtccg ctcaacttcc
     1681 gcggcgtgcg cattggcgtg ccgatttgcg aggatatttg gcaggaggag atctgcgaat
     1741 gcctgcagga gacgggcgcc gagctcttga tctctccgaa cggctcgccc ttcgactgga
     1801 cgaagcccga cctgcgcatg aacgtggcgg tggcgcgcgt taccgaaaac gccttgcccc
     1861 tcatctacgt caaccaggtc ggcggccagg atgaactcgt gttcgatggc gcctccttcg
     1921 ttctcaatgc ggatcgcgct ctcgccgtgc agctggcggc ctgggaggag tgcgtcggcc
     1981 tgatcacgct caatcgctcg gcccagggct ggaacgcgga gcgccagccg ctcagcctgc
     2041 tggaggagca cgaggcggcc gcctatcacg cttgtgtgct gggcttgcgc gactacgtcg
     2101 acaagaaccg cttccccggc gtggtcatgg gcctctcggg cggcgtggat tcagccctgg
     2161 tcgcggccat ggcggtcgat gcgctcggcg cccagcgcgt gcactgcatc atgctgccct
     2221 accgctatac gtccaatgag agcctgtcgg acgcggccgc ctgcgccgag gcgttgggcg
     2281 tgcgctatga caccattccc attgcgccgg ccgtcgaggg gttcggggcg atgttggagg
     2341 cgcaattcgc cgggcgggcc aaggacatta ccgaggagaa cgtgcagagc cgggtgcgcg
     2401 gcacgacctt gatggcgctg tccaacaagt tcggctcgct ggtgctgacg accggcaaca
     2461 aaagcgaaat gtcggtcggc tacgccaccc tctacggcga tatgaatggc ggctttaatc
     2521 cgatcaagga cctatataag accgaggtct accgcctgtg tgcctggcgc aatcggcacc
     2581 atcccaaggg cggcaaaggg ccgagcgtcc cggttattcc cgagaatatt ctgaacaagg
     2641 cgccgacggc ggagctgcgc gagaaccaga aggatcaaga tagtcttccc ccctacgagg
     2701 tgttggacga catcttggcc tgtctggtcg agcaggaatt gggcttggcg gacatcgtcg
     2761 cccggggaca tgcgcccgca atcgtcaaaa aggtcgagca ccttttatat ctggctgagt
     2821 ataaacgccg ccaggccgcg cccggggtca agatttccgc gcgcaacttc gggcgcgatc
     2881 ggcgctatcc catcaccaat aagtttcgcg aaaagcttgg cgagagctag tccagcaggc
     2941 gggcaaccgc ttaacggcgc gccgtagacg cgcgcctgaa cgcgacgagc gcgagaccat
     3001 gttgcgaggg gctggaaata ggaaaagatc agggcgtccc gaggtgcggg gatttcggga
     3061 cgccctaatt ttgatccgcg gtgcgggggt gcggatcgtc ttcaaaacga atgagtggaa
     3121 accatattcc caagatccgc gaaaaaattt gcagcgcttt tacgattggg ccccggaccc
     3181 gggcgtgcgc tcgctggcgg ggccgagaaa tacggtgcgg atgaactctc ggtagagcag
     3241 aatctgtcgc ggcaggatgg ttttatcgac aagtacgcgc gacatgatga tgccgccatc
     3301 caccagcgtg ttggccatgt cggcaaggtc ttccagggat agggccacct tgggtggata
     3361 gcgtccggcg attatttcca gacggtcgag aaacaatttc cgccagctgc ggacgcccgt
     3421 ctgggtcagc gtgcgcacct cgccgtcgaa caggcgatcc tgatagcaaa aggaggccac
     3481 caggcagccc gggtgcgtcg ccggcagctc ggccatcatt tcggcgaaga gcttcagggc
     3541 gaccaggaag ccgtgcagcg ggtcctcatt gagctcgtcg gcgcgcacga aaatgtcgtc
     3601 aagtacggcg tcgttgtggg cgatgtagcg cagcatcaga gcgcgcgcga gctcaccctt
     3661 atctcgaaaa tgataaaaga agccgctctt ggtgatgccg gccccggcga tcaattcctc
     3721 gatcgaggtg gcggcaaagc ctttgtcgag gacggccgct tcggcgagct caaggatgcg
     3781 ctcgcgcgtc gcgttcgtgc gggggactgc ggcggcatcg gccatgccac tgtactaacg
     3841 gtagagtcgt ttaggggcaa ttggggcaaa acctggcgag cgctcatcct tgcgtcggcc
     3901 taaacccctg atctccttca taaagcattc gtgaccggga ctgcaccaga ggtgggctgg
     3961 gaagctccca ccgcattgcg gcaagatgcc ggcgcatcga aa
//
LOCUS       DASTMT010000102         3140 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_383807, whole genome shotgun sequence.
ACCESSION   DASTMT010000102 DASTMT010000000
VERSION     DASTMT010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3140
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_383807"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            578..1117
                     /locus_tag="VFR00_02140"
     CDS             578..1117
                     /locus_tag="VFR00_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011511304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HEU0158078.1"
                     /translation="MSKVRTLEPQSKIAAPLDTPTDLADEAVREISAALNRLLADSFA
                     LYLKTKNFHWHVSGPHFRSYHLLFDEQAAEIFATTDELAERVRKIGGSTLRSIGDIAR
                     QQTVKDNDKAFVAPGEMLRELMADNKAMAAHMRKAHQVADKYEDVATASVLENFIDAS
                     EKRTWFLFEAGRKADDSGH"
     gene            1409..2266
                     /gene="mazG"
                     /locus_tag="VFR00_02145"
     CDS             1409..2266
                     /gene="mazG"
                     /locus_tag="VFR00_02145"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018265375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HEU0158079.1"
                     /translation="MPTEAKKSLAELLDIMAALRAPATGCPWDLAQTFATIAPYTIEE
                     AYEVADAIERGDPAALREELGDLLLQVVYHARMAEEQGAFAFADVVDAIAQKMLRRHP
                     HVFGSAAERAAGAAPGFWERIKDAEKAQERPGAGVLDAVPVGLPALTRAVKLQQKAAS
                     VGFDWPSRQQVLDKFKEELGELEAALAAPTTGSPRQQEAIAEEMGDLLFVMANLARHL
                     KLEPEMILRQANAKFARRFAGIELKLAAAGKTPQQSDLAEMDRFWQEVKAEEDKAEEE
                     EEEEEEEEA"
     gene            complement(2323..>3140)
                     /gene="hflX"
                     /locus_tag="VFR00_02150"
     CDS             complement(2323..>3140)
                     /gene="hflX"
                     /locus_tag="VFR00_02150"
                     /inference="COORDINATES: protein motif:HMM:TIGR03156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEU0158080.1"
                     /translation="RDRMAKIRRELDQVKRTRTLHRDRRQRAPWPVVALVGYTNAGKS
                     TLFNRLTGEAVFAENLLFATLDPTMRDIKLRGFDKVILSDTVGFVSDLPTELIAAFRA
                     TLEEVIAANLILHVRDVSHPESEAQYHDVVAVLGDLGIDADSDGAPILEVWNKADKLA
                     PGGRTEVEQALSRRNRPAVLVSALTGEGMETLRAQLDTELGRHDEVFDLEIPADQGRL
                     LSWLHANAEVLDQKTEGSGAVLARIRIEPAAKGKLAGQLKRAGLSLNGMDDRK"
BASE COUNT          576 a          988 c         1032 g          544 t
ORIGIN      
        1 tcctcggccg cctgctgcga aacagccggg caatccgcga gtgggccgat gacttcggcg
       61 cggcgccggg cttcgcgcca ctgctcgtcg ttcaaagtca acgggcctcg gtctgcgggt
      121 tgatcggaat catcgttcat ttgaaaattc tgtcgccgcg ctgacagttc atggtttgtt
      181 ccgtaacacc aattggacgc ggcatcaagg ataggcgtca ggtaacgccg acgcgaaacg
      241 aacagcgaca agccggccgt gtcggagaaa accgcataca cagttgcacc gagcttaggg
      301 ccaattggcc cgctatcacg gatcagcgac tgcccagccg tatcggggaa gaccgcatag
      361 gcaatggagt gagcttttgg ccaaccccgg agcatagtga agtctgcttc tggatatctc
      421 gacaggcaga agtgaatttc agtggggctt ctgatctagg attccttgtg aaatcgtctt
      481 ttacggacga caggaacgct cggcgctcca gcgcgttttt cttcggcggc atgccgcccg
      541 ccgccgatcc aaccagcaag ccccggagtt gttagtcgtg agtaaggtca ggacgcttga
      601 gcctcagagc aagattgctg cccccctcga tacccccacg gatcttgccg atgaggccgt
      661 gcgcgagatt tccgccgcgc tcaatcggct gttggctgat agctttgcgc tctacctcaa
      721 gaccaagaat tttcattggc acgtcagcgg cccgcatttt cgctcctacc acctgttgtt
      781 tgacgagcag gcggccgaga tcttcgccac caccgacgag ctcgccgagc gcgtgcgcaa
      841 gatcggcggc agcacgctgc gctcgatcgg cgacattgct cgccagcaaa cggtcaagga
      901 caacgacaag gccttcgttg ctccgggcga gatgttgcgc gagctgatgg ccgacaacaa
      961 ggccatggcc gcgcacatgc gcaaggccca tcaggtggcc gacaagtacg aggacgtagc
     1021 gaccgccagc gtgcttgaga acttcatcga tgccagcgaa aagcgcacct ggttcttgtt
     1081 cgaggcgggc cgcaaggccg acgacagcgg ccactgaaat cccggcgtga aagttgggtc
     1141 gcgacgcccc gttggaactc gccccgcggc ccaaagcccc ttgcagagcc tcccttgcag
     1201 agcgctcccc tggcaacgcg gtgcgcaatt ttcgccgtgc gccccccgcg gggaaaggcc
     1261 gggtgctgcc aagtggtgct tgccttggcg cgcaggtttt gtgctaaggc gtcggctgcg
     1321 cgttaaacaa ggctcggctg gcgccgggca aggctgccgg cagcgttgag gcgcgcggcg
     1381 tcaggagctg aggcgagggg aggcgcagtt gccgacagag gcaaaaaaat cgttggccga
     1441 gctgctcgac atcatggccg ccctgcgcgc gccagcgacg ggctgcccct gggatcttgc
     1501 ccagaccttc gcaacaattg ccccctatac gatcgaggaa gcctacgaag tcgccgacgc
     1561 catcgaacgc ggcgacccgg cggcgctacg ggaggagttg ggcgacctgt tgctgcaggt
     1621 cgtctaccac gcccgcatgg ccgaggagca gggcgctttt gcctttgccg acgtcgtcga
     1681 tgccatcgcc cagaaaatgc tgcgtcgaca cccgcatgtg ttcggcagcg cggcggagcg
     1741 cgcggcgggt gcggcgcccg gattttggga gcgcatcaaa gacgcggaaa aggcgcagga
     1801 gcgcccgggc gccggcgttc tcgatgctgt gccggtgggg ctcccggccc tgacgcgcgc
     1861 ggtcaagttg cagcaaaaag ccgcaagcgt cggcttcgac tggccctcgc gccagcaggt
     1921 gttggacaag ttcaaagagg agctcggcga gctcgaagcc gcgctcgccg cgccgaccac
     1981 cggctcgccg cgccagcagg aggccatcgc cgaggagatg ggcgatctcc tgttcgtcat
     2041 ggccaacctg gcgcgtcacc tcaagcttga gcccgagatg atattgcgtc aggccaacgc
     2101 caaattcgcc cgcaggtttg ccggcatcga gctaaaattg gcggccgcgg gcaagacgcc
     2161 gcaacagtcc gatctcgccg aaatggatcg gttttggcaa gaggtgaagg ccgaggagga
     2221 caaggccgag gaggaggagg aggaggagga ggaggaggag gcttgagcgg agccaacggc
     2281 gagccggggg ccttcagctt gcccgtggcg ccatcccggc cgtcacttgc ggtcgtccat
     2341 gccgttgagc gacaagccgg cgcgctttag ctggccggcg agcttgcctt tggcggcggg
     2401 ctcgatgcgg atgcgcgcga gcacggcgcc cgagccttcc gtcttctgat cgagcacctc
     2461 ggcgttggcg tgcagccagc tcagcaggcg tccttgatcg gccgggatct caagatcgaa
     2521 cacttcatcg tgacgaccga gctcggtatc gagctgagcc cgcaaggtct ccatgccctc
     2581 gccggtcagg gcggatacca gcacggccgg ccggttgcgg cggctgagtg cctgctcgac
     2641 ttccgtccgc ccgcccggcg ccagcttgtc ggccttgttc cacacctcga ggatgggcgc
     2701 gccgtcgcta tcggcgtcga tgccaaggtc gcccagcacc gccacgacgt cgtggtactg
     2761 ggcctcgctt tcgggatggc tcacatcgcg cacgtgcaag atcaggttgg cggcaatgac
     2821 ttcctccagc gtcgcccgga aggcggcgat cagctcggtc ggcaggtcgg acacgaaccc
     2881 gaccgtgtcc gacaggatca ccttgtcgaa acctcgcagc ttgatgtccc gcatggtcgg
     2941 gtcgagcgtc gcgaatagaa ggttctcggc aaagacagct tcgcccgtca gcctgttgaa
     3001 aagcgtcgat ttcccggcgt tcgtatagcc gaccagagca acaacgggcc acggggcccg
     3061 ctgacggcgg tcacgatgca atgttcgggt ccgcttcacc tgatcgagct cgcggcggat
     3121 tttcgccata cggtcgcgga
//
LOCUS       DASTMT010000103         3907 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_4951233, whole genome shotgun sequence.
ACCESSION   DASTMT010000103 DASTMT010000000
VERSION     DASTMT010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3907)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3907)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3907
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4951233"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..258)
                     /locus_tag="VFR00_02155"
     CDS             complement(<1..258)
                     /locus_tag="VFR00_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012631158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0158081.1"
                     /translation="MKLAMKRINRGTTVLAAGASVLLVALSGGVEAQAPASYKFGLAM
                     PLSGSQALYGADQVKAAQWAVDAINAKGGINGKKLEMITLDT"
     gene            complement(298..1014)
                     /locus_tag="VFR00_02160"
     CDS             complement(298..1014)
                     /locus_tag="VFR00_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007594416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HEU0158082.1"
                     /translation="MSPTKKPWDGIISPEEQRAYAAAGFGRRTGLGQRPALLIIDVQY
                     RTVGSSPKPFWQAIEEFPTACGEVGWRAVAHIARLLALFRERDWPVLYPYVSPKESFD
                     KGRLADKVPAIMTIARNGYDFVAEVAPRENDILLPKKHPSAFFGTPLASYLINAGADT
                     LVVTGCTTSGCVRGSVVDAFAFNFRVAVPHDAVYDRSKTSHAVNLFDMSEKYADVSST
                     DELLGELAKIPRASNATRGS"
     gene            complement(1011..1913)
                     /locus_tag="VFR00_02165"
     CDS             complement(1011..1913)
                     /locus_tag="VFR00_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009756587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HEU0158083.1"
                     /translation="MMRNTTRLKSILARRDATLAPGAANALFARVIEDLGFDVVYVSG
                     AGIANMHLGMPDIGLTTLSEIVDTVGAIADVVALPLLVDADTGFGNALNMRRTVRLLE
                     RAGAAGVQIEDQIFPKKCGHFSGKSVVALPEMLQRIKAAVDCRHDQDFQIVARTDARA
                     IEGLEAAIARAHAFTAAGADATFVEAPLTEPELARIAGELAAPQLANIVFGGLTPDIG
                     RTKLAAMGFSLVLYANASLQAALKASQDVLAALKRDGSLHAVADRLASFSDRQRAVAK
                     PSYDALEARYAVPAPDRPAKGGRA"
     gene            complement(1910..2632)
                     /locus_tag="VFR00_02170"
     CDS             complement(1910..2632)
                     /locus_tag="VFR00_02170"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEU0158084.1"
                     /translation="MVVYSRQRPPLHTIPRETLADRVFEQIVEAIVQGRIAAGETINE
                     VNLSARLGVSRAPLREAIFRLEAKGLIQRTPHLGARVVDLKPRDLEELFLIREGLEGM
                     AAGLAALAMSDADIEALERSLEAHRRQPDVASGTAYYQAGGDEDFHFRIAKGSSNRRL
                     FRLLCEDMYSLIRVYRFRSSATPGRARQAYAEHQDILGALQRRDAEAAEAAMRRHIRL
                     SWSNIEARLSTPAAGSTQDVAS"
     gene            complement(2764..3582)
                     /locus_tag="VFR00_02175"
     CDS             complement(2764..3582)
                     /locus_tag="VFR00_02175"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU0158085.1"
                     /translation="MNTNLFEQRVLPHHQTPASIWGQGGSAYDDISFGICDALAHTAQ
                     RLSAKPKEQVLDVATGTGWSARNVARSGAEVTGVDISPALLAAAQDLSAHVRPVIRFE
                     LGDAERLPFADGRFDAAISTFGVIFAQDQERAALELGRVLRKGGRVALATWPPSGSVA
                     EFLAVIARHSNTPPPRVSPLSWGEPGHVESLLGRDFELSFEHGVNHAYHPDPTHIWDK
                     YALGFGPLRQLIGSLDAAQLGALRADVDAYHQAFATAVGLHVRREYVIILGRRR"
BASE COUNT          670 a         1231 c         1305 g          701 t
ORIGIN      
        1 cgtgtcgaga gtaatcatct ccagcttttt gccgttgatg ccgcccttgg cgttaatggc
       61 atcgacggcc cattgcgcag cctttacctg atcggcgcca tagagcgcct gtgagccgga
      121 caaaggcatg gcgagcccga atttgtagct cgcgggtgcc tgggcctcaa cgccgcctga
      181 cagggccacg agcagcacac tggcgccggc cgcgagcacc gtcgtgcccc ggtttatgcg
      241 cttcattgca agtttcatgt ggccaacctc ccttgagttg tcgagcgatg cgagaaccta
      301 gctgccgcga gtggcgttgc tcgcgcgtgg gattttcgcg agctcgccta gcagttcatc
      361 ggtgctggac acatcggcat acttctccga catgtcgaat agattgacgg catgcgaggt
      421 cttgctgcgg tcgtaaaccg catcgtgcgg tacggcgacg cgaaaattga aggcgaatgc
      481 gtcgacgacg ctgccgcgca cgcatcccga cgtggtgcaa cccgtcacca ccagggtatc
      541 ggcaccggcg ttgatcaggt aactcgcgag cggggtacca aagaaggcac tgggatgttt
      601 cttcggcagg agaatgtcgt tctcgcgggg tgcgacctcg gcgacaaaat cataaccgtt
      661 gcgggcaatg gtcatgatgg cagggacctt atcggcaagt cggcctttat cgaagctctc
      721 cttgggtgag acgtaggggt agaggacggg ccagtcacgc tcgcggaaga gtgcgagcaa
      781 acgcgcaatg tgcgccaccg cgcgccagcc aacctcgccg caagccgttg gaaactcctc
      841 gatcgcctgc caaaacggct tggggctgga gcccacggtg cgatactgca cgtcgatgat
      901 cagcagggcg ggtcgctggc ccaggccggt gcggcggcca aagccggcgg cggcgtaggc
      961 gcgctgctct tccggcgaaa taataccatc ccagggtttc ttggttgggc tcatgctcgg
     1021 cctccctttg ccgggcgatc gggcgcgggc acggcgtagc gtgcctccaa tgcgtcgtag
     1081 ctcggcttgg ccaccgcgcg ctggcggtcg ctgaaactcg cgagccggtc ggcgacggca
     1141 tgcaaggatc catctcgctt cagcgccgcc aagacgtcct gggatgcttt gagcgccgcc
     1201 tgcagcgagg cattggcgta aagcaccaag ctgaagccca ttgcggccaa cttcgtgcga
     1261 ccgatatccg gggtcaggcc gccaaagacg atattggcga gctgcggcgc ggcaagctcg
     1321 ccagcgatgc gggcgagctc tggctccgtc agaggcgctt ccacgaaggt cgcatccgcg
     1381 ccggcggcgg tgaaggcatg cgcgcgtgcg atcgcggctt cgagcccttc gatggcgcgc
     1441 gcatcggtgc gcgcgacgat ttgaaagtcc tggtcgtggc ggcaatccac ggcggctttg
     1501 attctctgca gcatttcggg gagcgcaacc accgacttgc cggaaaagtg gccgcacttt
     1561 ttgggaaaga tctggtcctc gatttggacg ccggccgcgc cggcgcgctc cagcaggcgt
     1621 acggtacgcc gcatgttgag cgcattgccg aagccggtat cggcatcgac caagagaggg
     1681 agcgccacca catcggcaat agcgccgacg gtatcgacga tctcgctcaa cgtggtgagc
     1741 ccgatgtccg gcatgcccaa atgcatgttg gcaatgccgg cacccgagac atagacgacg
     1801 tcgaagccta gatcttcgat gacgcgggca aatagcgcat tggccgcgcc gggtgccagg
     1861 gttgcatcgc ggcgcgccag aatagatttg aggcgtgtgg tgttgcgcat catgaggcga
     1921 catcttgcgt ggagcccgcg gcgggggtgc tgagccgggc ttcgatattg gaccaggaca
     1981 gccgaatgtg ccggcgcatc gccgcctcgg cggcctccgc atcgcgacgt tgcagcgcgc
     2041 ctagaatgtc ttggtgctcg gcgtaggctt ggcgtgcgcg gcccggcgtg gcgctgctac
     2101 ggaaacggta aacgcggatc agcgagtaca tgtcctcgca caacaggcga aacagccggc
     2161 gattgctgct gcccttggca attcggaaat gaaagtcctc atcacccccg gcttggtaat
     2221 aggcggtgcc gcttgccacg tccggctgtc ggcgatgcgc ttcgaggctc cgctcgagcg
     2281 cttcgatatc cgcatccgac atggcaagcg cggccaaccc ggccgccatg ccttcgagac
     2341 cctcgcggat caggaaaagc tcctcgaggt cgcgcggctt gaggtcaacg acgcgtgcgc
     2401 ccagatgcgg tgtgcgctgg atcaggccct tggcttccag ccggaagatg gcctcgcgca
     2461 agggggcccg gctgacgccc agccgggccg acaggttgac ttcgttgatg gtttcgccgg
     2521 cagcgatgcg cccctgcacg atcgcctcca cgatttgctc gaacacgcga tcggcaaggg
     2581 tttcgcgggg aatagtgtgc aggggcggcc gctgtcgact gtatacaacc atggcccatc
     2641 aaagcatttc gggtctggcc ctggcaagcg caccgccgcc aaagccgccg ttcgcttcgg
     2701 tccctcgcca tggggcgctc gcgcggggcc acgccggtgc gccctcagcc cctggggccc
     2761 aagttagcgt cgccggccca ggatgatcac gtactcccgt cgcacgtgca ggccgaccgc
     2821 ggtggcgaac gcctgatgat aggcgtccac atccgcgcgc agggcgccga gctgggcggc
     2881 gtcgaggctg ccgatgagtt gccgcagggg cccaaacccc aacgcatact tatcccagat
     2941 gtgggtcgga tcaggatggt aggcatgatt gacgccgtgc tcgaagctca gctcaaagtc
     3001 acgcccgagc agggattcga cgtgcccagg ttctccccag ctcagcgggg agacgcgcgg
     3061 cggtggcgtg ttactgtgac gggcgatcac cgccaggaat tcggcgaccg aaccgctcgg
     3121 cggccaggtc gcgagcgcca ccctgccgcc cttgcgcagc acgcggccga gctccagggc
     3181 cgcccgttcc tgatcttggg caaagatcac cccgaaggtg gaaattgccg catcaaagcg
     3241 gccgtcggca aacggcaggc gctcggcgtc acccaactcg aacctgatca cgggtcggac
     3301 gtgagccgac aggtcttggg ccgcggccag caacgcgggc gaaatatcga cccctgtcac
     3361 ctccgcgccc gagcgggcga cgttgcgcgc cgaccagccg gtgccggtgg caacgtcgag
     3421 cacctgctcc ttcggcttgg cgctgagccg ctgcgcggtg tgcgccaagg catcgcagat
     3481 cccgaagctg atgtcgtcat aggcgctgcc gccctgaccc cagatcgaag cgggggtttg
     3541 atgatgtgga agcacgcgct gctcgaatag gttggtgttc attgcatcct ctggcgcttg
     3601 ccgctttccc tgggctgagg cgacgcatcg gcctgttgcc gttcgttacc gacttttccc
     3661 aggggcggtt gcacgccatg ctagcggggc cgaggctgga aacctttgcc gcaaatccgc
     3721 gcttttttgc aggagtgggt tagcggcggg aggagtgggc gatggacgca ctttcccaag
     3781 cgctgagctc tgtccgcatg accggtgcca tttttgtcga cgccctatgc accgcccctt
     3841 gggggttttc cgttccggcg atggcgcggg tggctcatct gctggcaccc ggcaccgagc
     3901 acctcgt
//
LOCUS       DASTMT010000104         5502 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_3637058, whole genome shotgun sequence.
ACCESSION   DASTMT010000104 DASTMT010000000
VERSION     DASTMT010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 5502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5502
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3637058"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1391)
                     /locus_tag="VFR00_02180"
     CDS             complement(<1..1391)
                     /locus_tag="VFR00_02180"
                     /inference="COORDINATES: protein motif:HMM:NF025873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEU0158086.1"
                     /translation="MHLLYGILRQSHAQGTHHKLALDALSQLEGPDRAPWQRLFLRHA
                     ELYLEGAKAPDRDFKDYKNHVLHTRDDYWGGAPQKVVSWYQHLVEGLAQEDWQTAVYC
                     AGVLSHYYTDPLHPFHTAQSEAENNIHRAVEWSIAKSYEELRAQGERDFPHMPIEVTA
                     DANWLATLLVRGAQRANAYYEKLIAHYDIERGVVDPPAGLDRIARRIVAELIRYASLS
                     YAIVLARAIEEARVRPPAVPLTAATLLAGLKIPFKLIERHRADRGERRDIERSYDELI
                     AAGKVETTLREEERSVRDLHAAEVVAKRQPPEASKVMTFRPRPRVVTHIERTRAARLA
                     VAAQDNVVAFNALRPPPLGAAAAGAEPPPRARAGLTLRLQQVTAAPRRRPAAPAASAP
                     AQRLSLDQDVVLGPSIGPKTAARLYPHGIKTVRDLIKADPTALAVLIGARHITPEIIS
                     SWQDQARLVCTLPG"
     gene            1592..2074
                     /locus_tag="VFR00_02185"
     CDS             1592..2074
                     /locus_tag="VFR00_02185"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEU0158087.1"
                     /translation="MSLSDDEIRALLADAAQQPPSARHLNFELLAFSVEEAWAEVAFT
                     PRPEFANPAGHVQGGFVCAMLDDAMGFTASISQRFAIVVPTLQVCVSYLRPTPIARVI
                     ARGEVLRMGNATAHLQGVLRLPDGTALATATATAALRPFPRPAASATAPRGETGRRQG
                     "
     gene            complement(2071..2241)
                     /locus_tag="VFR00_02190"
     CDS             complement(2071..2241)
                     /locus_tag="VFR00_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011924935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0158088.1"
                     /translation="MSEDNARPRIGAELAKIEAEPLLPIEKKLLAWSLIAGVVLLGLL
                     VWTGNSALPLGH"
     gene            complement(2254..3738)
                     /locus_tag="VFR00_02195"
     CDS             complement(2254..3738)
                     /locus_tag="VFR00_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008968698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HEU0158089.1"
                     /translation="MAEQTALRGDLAVSPPAASLSEDWLAVVVGGLIFLFALALLTGV
                     DLIGWATAPRTWVELGKSVRPASALYAATSPPALLLATYLSVLALLSLGAFLLGSNLV
                     KFMLGFSVIYWLSYLAWLAGNYAYVAVTTPVEMQRLGIGWSLKLTGEAGYLIALLLGL
                     AIANVAPPLARWLGEAARPELYIKTAIVILGGFLAVKAAEQLSLASTVMLRGLAAIIE
                     AYLIYWVVVYFIARKWFGFSREWAAPLASGISICGVSAAIATAGAIRARPLVAIMVSS
                     LVVFFAMVELLILPFLARSFLADQPLVAAAWMGLAVKTDGAAVASGAVTEALLYGDAA
                     ARGINWSPGWVLGTTTTIKVFIDIFIGVWSFLLAYLWTRHIEPRRAGDELRFVEIWQR
                     FPKFVLGFLATFTIVLGIGLAAGPATRKTLDGAMTEANVFRQLFFLFTFFSIGLMSDV
                     RQLWQEGIGKLAAVYAVSLFGFVIWVGLLISWLFFYGIMPPLAR"
     gene            complement(4015..>5502)
                     /locus_tag="VFR00_02200"
     CDS             complement(4015..>5502)
                     /locus_tag="VFR00_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0158090.1"
                     /translation="GGERVTLKIRREGRPDETRSTSIGRTVQIQMPFPHAGQNIVEIE
                     LETAPGELTAANNRVVVAAEGVRENLRVLLVSGEPHAGERVWRNLLKSDAAVDLVHFT
                     ILRPPQKQDGTPIHQLSLIAFPTRELFSEKIKDFDLIIFDRYQHRDILQLLYYENIAR
                     YVEQGGALLVAAGDDFAGQMSLSRTPLATVLPATPTGRLLEQPFKAKLSLDGLKHPVT
                     RGLPGSPSEANKDPSWGRWFRQVDVIPGKGRTVMNGVDNHPLLVLDRKGRGRVALLLS
                     DHAWLWARGYDGGGPHTDLLRRLAHWLMKEPDLEEERLLASSKGLKLTIERRSMEESV
                     PEVQVSAPGGETSSVNLEPAGPGAWRSTIDVKTPGLYKLQTASPTGQLTAVAHTGIED
                     AREMSEVTATDEKLKPLVEATGGGVFWTKGGGLLAAMSPSAVEVPRISMLANARVLAG
                     SGWLGLKDREAYVTRGVKLIPMFTGALALAALLAFLALAWWREGR"
BASE COUNT          948 a         1791 c         1914 g          849 t
ORIGIN      
        1 cccggcagcg tgcagacgag ccgcgcctga tcctgccagc tcgaaatgat ctctggcgtg
       61 atgtggcgcg ccccgatcag caccgccagc gccgtcggat cggctttgat gaggtcgcgc
      121 acggtcttga tgccgtgcgg ataaagacgc gctgccgtct ttggaccgat cgaggggccg
      181 agcaccacgt cctgatcgag cgaaagccgc tgcgccgggg ccgatgctgc cggcgcggcg
      241 gggcgtcgtc gcggcgcggc ggtgacctgc tgcaagcgca gcgtcagccc ggcgcgggcg
      301 cgcggcggcg gttcggcgcc ggcggccgca gcgcccagcg gcggcgggcg cagggcattg
      361 aaggccacca cgttgtcctg ggcggccaca gccaggcggg cggcgcgcgt gcgctcgatg
      421 tgggtgacga cgcgcgggcg cggccggaag gtcatgacct tcgatgcctc gggcggttgg
      481 cgcttggcca ccacctcggc ggcgtgaaga tcgcgcacgg agcgctcttc ctcgcgcagc
      541 gtggtttcga ctttgcccgc ggcgatcagc tcatcgtaac tgcgctcgat gtcgcgacgc
      601 tcgccgcgat cggcgcggtg ccgttcgatc agcttgaacg ggatcttgag gccggccagc
      661 agcgtcgcgg ccgtcagcgg caccgcgggt gggcgcacgc gggcttcctc gatggcgcgc
      721 gcgagcacga tcgcgtagct gaggctggcg tagcggatca gctcggcgac gatgcgccgc
      781 gcgatccgat cgagccccgc cggcgggtcg accacgccgc gttcgatatc gtagtgggcg
      841 atcagctttt cataataggc gttggcgcgc tgagcgccgc gcacgagcag tgtggcgagc
      901 cagttcgcgt ccgccgtcac ctcgatgggc atgtgcggga agtcgcgctc gccttgggcg
      961 cgcagctctt catagctttt ggcgatgctc cactcgacgg cccggtgaat gttgttttcc
     1021 gcctcggact gggcggtatg aaagggatgc aacgggtccg tgtagtagtg gctcagaacg
     1081 ccggcgcagt agaccgccgt ctgccagtct tcttgggcca agccctccac caggtgctga
     1141 taccagctca cgaccttctg cggcgcgccg ccccagtagt cgtcgcgcgt gtgcagcacg
     1201 tggttcttgt aatccttgaa gtcccggtcc ggcgccttgg cgccttccaa atagagttcg
     1261 gcatgccgca ggaacagccg ctgccagggc gcgcgatcgg ggccctcgag ctggcttagc
     1321 gcatcgagcg ccaatttgtg gtgggtgccc tgggcgtggc tctgcctgag gatgccgtag
     1381 agaaggtgca tggcggtcgc attcccctga gcaaaagtcg gcacgcacaa cgggacggcc
     1441 cggaaagccg gcacgatagg gcgagtcgcc cgctttcaac aggcgctagg ccgccgctgc
     1501 cggcggcgca tctgagcgtt gcctcacccg ggaaacagcg gctatttggc ctctggcgcg
     1561 ctgggcgcct gagccaacgg acagacccga catgagcctc tcggatgacg agatccgcgc
     1621 cttactcgct gacgccgcgc agcagccacc cagcgcgcgc catctcaact tcgagctctt
     1681 ggctttttcg gtcgaggagg cctgggcgga ggtcgccttc accccgcgtc ctgagtttgc
     1741 caatcctgcc ggtcacgtgc agggcggctt cgtctgcgcc atgctggatg acgccatggg
     1801 cttcaccgcc agcatctcgc agcgctttgc cattgtggtc ccgaccctgc aagtctgcgt
     1861 aagctatctg cggccgacgc cgatcgcgcg cgtcattgcg cgcggcgagg tcttgcgcat
     1921 gggcaacgcc acggcccacc tgcagggcgt gctgcggttg ccggacggga cggcgcttgc
     1981 caccgccacc gccacggcgg cgctcaggcc gttcccgcgc ccggctgcgt cggccacggc
     2041 gccgcgtggc gagacgggca gaaggcaggg ctagtggccg aggggcaggg cggaattccc
     2101 ggtccacacc aacagcccca gcagcaccac gccggcaatc aggctccagg ccagcagctt
     2161 tttttcgatc ggcagcagcg gctcggcctc gatcttggcg agctccgcgc cgatgcgtgg
     2221 gcgtgcgtta tcctcagaca taattcgtct ccctcagcgg gcgagcggcg gcatgatgcc
     2281 gtagaagaag agccacgaga tcagcaagcc aacccagatc acgaagccaa acagcgagac
     2341 ggcataaacg gcggccaact tgccgatgcc ttcttgccag agctggcgca cgtcgctcat
     2401 caagccgatc gaaaagaagg tgaagaggaa gaagagctgg cggaagacgt tggcttccgt
     2461 catggcgccg tcgagggttt tgcgcgtggc cggcccggcg gccaggccga tgcccagcac
     2521 aattgtgaag gtggcgagaa agcccaagac gaacttggga aagcgctgcc aaatctcgac
     2581 aaaacgtagc tcgtcgccgg cgcggcgcgg ctcgatgtgg cgggtccaaa ggtaggccaa
     2641 caggaaggac cagacgccga tgaagatatc gatgaagacc ttgatggtgg tggtggtacc
     2701 gagcacccag ccggggctcc agttgatgcc ccgggcggcg gcatcgccgt aaagcagggc
     2761 ctcggtcacg gccccgctgg ccacggcggc cccatcggtc ttcaccgcca agcccatcca
     2821 ggccgccgcc accaagggct gatcggccag aaagctgcgc gccaggaacg gcaagatcaa
     2881 cagctccacc atggcaaaga agaccaccag cgaggagacc atgatcgcca ccagcgggcg
     2941 ggcgcgaatg gccccggcgg tagcgattgc cgccgagacg ccgcagatgg agatgccgga
     3001 ggcgagcggt gctgcccact cgcgcgaaaa accaaaccat ttgcgagcaa tgaaatagac
     3061 caccacccag tagatcagat aggcctcgat gatggccgcc agcccgcgca gcatcacggt
     3121 cgaggccagg ctcagctgct cggcggcctt caccgcgaga aagccgccga gaatgacgat
     3181 cgccgttttg atgtagagct cggggcgggc cgcctcgccg agccagcgcg ccaagggagg
     3241 tgcgacgttg gcgatggcaa gacccaggag cagcgcgatc agatagcccg cttcccccgt
     3301 cagcttcagc gaccagccga tccccagccg ctgcatttcc acgggggtgg tgacggcgac
     3361 ataggcgtag ttgccggcga gccaggcgag ataggacagc cagtagatca cgctgaaacc
     3421 cagcatgaac ttgacgaggt tgctgcccag cagaaaagcg ccgaggctca gcagcgccag
     3481 cacgctgagg taggtggcga gcaggagagc cggcgggctg gtggcggcat agagcgcgga
     3541 ggcaggccgc acggacttgc cgagctcgac ccatgttcgc ggcgccgtcg cccagccgat
     3601 gaggtcgacg ccggtgagga gcgccagggc gaaaaggaaa atgaggccgc cgacgacgac
     3661 cgccagccaa tcctcgctga gcgaggcagc cggtgggctc accgccaggt cgcccctgag
     3721 cgccgtctgc tctgccatgc tcgcctcccc ctgggaaatc caccaatgag taaaaagatt
     3781 ttctaagatt cggcaagaag gtgcgagaaa aagaaaaatc atacccatgc gtgccgagcg
     3841 ccgcgggcgg tccgttgccg cgccgaggct cggcgtggca atgccgcggc gcgccaaggc
     3901 ttggcccgag caaaccgttg tggcgcgagc cgaggcaatt ctgggcaaag cccgggcaaa
     3961 ggcgggggcc cggcggcgcc gggccagtgg ggaccggaaa atctggagag cggcctagcg
     4021 gccctcgcgc caccaggcga gcgctaggaa ggccagcagc gctgccagtg ccaaggcgcc
     4081 ggtgaacatc gggatcagct tgaccccgcg cgtcacgtag gcttcgcgat ctttgaggcc
     4141 cagccaaccc gagccggcca gcacgcgcgc gttggcgagc atggaaatgc gcggcacctc
     4201 gacggcgctg gggctcatcg ccgccagcag gccgccgcct ttggtccaga acacaccgcc
     4261 gccggtggcc tcgaccaggg gcttcaattt ttcgtcggtc gccgtcacct cgctcatttc
     4321 gcgcgcgtcc tcgatgcccg tatgggcgac ggcggtgagc tggcccgtcg gtgaggccgt
     4381 ctgcagctta tagaggccgg gcgtcttcac atcgatggtg ctgcgccagg cgccgggacc
     4441 cgccggctcc aggttgaccg agctggtctc gccgcccggc gcggagacct ggacttccgg
     4501 cacgctctcc tccatcgagc ggcgctcgat ggtgagcttg aggcctttcg aggaggccaa
     4561 caggcgctcc tcctccagat ccggctcctt catcagccag tgggcgagac gccgcagcaa
     4621 atccgtgtgc ggaccgccgc cgtcgtagcc gcgcgcccac agccaggcgt ggtcggacag
     4681 cagcagcgcg acgcgtccgc gtcccttgcg gtcgagcacg agcaggggat ggttgtcgac
     4741 gccgttcatg acggtgcgac ccttgccggg gatcacgtcg acttgccgga accagcggcc
     4801 ccagctcgga tccttgttgg cctcgctcgg gctgccgggc aagccgcgcg tgaccgggtg
     4861 cttcaggcca tccaacgaga gcttggcctt gaagggttgc tcgagcaggc gccccgtcgg
     4921 ggtagcgggc aacacggttg ccagcggcgt gcgcgacagg ctcatttggc cggcaaagtc
     4981 gtcgccggcc gcgaccagca gcgccccgcc ctgctccacg tagcgggcga tgttctcata
     5041 atagagcagc tgcaggatgt cgcgatgctg gtagcgatcg aagatgatga gatcgaagtc
     5101 cttgatcttt tccgaaaaca gttcgcgcgt gggaaaggcg atgagcgaga gctggtggat
     5161 gggggtgccg tcctgctttt gcggcgggcg caaaatcgta aagtggacga ggtcgaccgc
     5221 agcgtcggac ttgagcaggt tgcgccagac gcgttcgccg gcgtgcggct cgccggagac
     5281 cagcaagacg cgcaggttct cgcgcacgcc ctcggcggcc accaccacgc gattgttggc
     5341 ggcggtaagc tcgcccgggg ccgtctcgag ctcgatctcg acaatgttct ggccggcgtg
     5401 cggaaacggc atctgaattt gcacggtgcg gccgatgctg gtcgagcgcg tttcgtcggg
     5461 gcggccttca cgccggatct tgagggtgac gcgttcgccg cc
//
LOCUS       DASTMT010000105         3019 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_1039724, whole genome shotgun sequence.
ACCESSION   DASTMT010000105 DASTMT010000000
VERSION     DASTMT010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3019
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1039724"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..858)
                     /locus_tag="VFR00_02205"
     CDS             complement(<1..858)
                     /locus_tag="VFR00_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012114336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HEU0158091.1"
                     /translation="MMARRATGKTRMARALWYVKKGSVELRTAPLPPPAPGHARVRSL
                     FSGISRGTERLVLLGAIGPSEWERMRAPMQEGAFPFPVKYGYAVTGIVESGPANLMGR
                     PVFALHPHQDYFNAPIEALIPIPEGLPARRATLAANMETALNAMWDSGAGPGDRIVIV
                     GAGVVGLLLAALAARLPGAIVTAVDVEEQRRPLAEGLGLGFARPEHAPTDADVVFHTS
                     ATGAGLDAAIRCAGLEAPVVEVSWYGDRPATLELGGAFHSRRLRLLSSQVGQISPSRR
                     PRWSFRRRLE"
     gene            919..2727
                     /gene="mdoH"
                     /locus_tag="VFR00_02210"
     CDS             919..2727
                     /gene="mdoH"
                     /locus_tag="VFR00_02210"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012334499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HEU0158092.1"
                     /translation="MSDSFDAPAAGALPAPALSLTTPTGLQSIDELARRRRIVLGLNV
                     ITYGLMLWWAGRILAAGGWTWVDGVLFACFALGTPWAVLGFWNALIGLWLLNVAGKHA
                     GMAVAPYAAAGAEPTPIRIKTAIFMTLRNEDAARAIQRFQIVQDSVERTGEGAAFSYF
                     VLSDSNDGAVAAAEEAAVAAWQAVAGAPDRITYRRRSSNVGYKAGNVRDFCARWGDDF
                     DLMLPLDADSLMSGAQIVRLVRMMQAHPKIGILQSLVVGMPSASAFARIFQFGMRHGM
                     RAYTMGQAWWVGDCGPFWGHNALVRVRPFYAECDLPLLPGKPPLGGHVLSHDQVEATL
                     MRRAGYEVRVLPEEVGSWEENPPTMLEFARRDVRWCQGNMQYLKLLDLPGLYPMSRFQ
                     LLWAILMFLGIPAWTLMIALLPLAAWQAQSVPEFPTGAAKALYATYFIMYLMPKIAGL
                     LDAMFGRGGVARYGGWLRFLISAVLELTFSFLQGAVSTIRTSIFMLGLLFGRSVVWGG
                     QVRDAQRLSWSEGARHLWPQTLFGVIVCGALAVIAPAVLWWSLPLTAGYLLAIPFAVI
                     TADPAFGRALKRFGICGIPEDFRPPEEIVATRATTT"
     gene            2738..>3019
                     /locus_tag="VFR00_02215"
     CDS             2738..>3019
                     /locus_tag="VFR00_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0158093.1"
                     /translation="MAIEDRVGALSNDPAFATLHRSLAVYYGHAARDAAMDRLYRRFL
                     GLGELAFDIGSHVGDRVGSFRRLGARVVALEPQPLCARAIRTIYADDNDV"
BASE COUNT          443 a         1003 c         1007 g          566 t
ORIGIN      
        1 ctcaagccga cgccgaaagc tccagcgcgg gcggcggctg ggcgagatct gtccgacctg
       61 ggaggacaga aggcgcaggc ggcggctgtg aaaggcgccg ccgagctcga gggttgctgg
      121 ccggtcgcca taccaactca cctcaacgac gggcgcctcg aggccggcac agcggatggc
      181 ggcatcgagg ccggcgccgg tggcgctggt atggaagacg acgtcggcat cggtgggtgc
      241 atgctcgggt ctggcaaagc cgagccccag tccttcggcc aaaggccggc gctgctcctc
      301 gacgtcgacg gccgtgacga tggccccggg caggcgggcc gcgagcgccg ccagcagcag
      361 cccgacgacg ccggcgccga cgatcacaat gcggtcgccc gggccggcgc cactgtccca
      421 catggcgttg agggcggttt ccatattggc ggccaaggtg gcgcgtcggg ccggcaggcc
      481 ctcaggtatc ggtatcaagg cctcgatcgg ggcattgaag tagtcctggt gcggatgcag
      541 cgcgaacact ggccggccca tgagattggc cggtccgctc tcgacgatcc cggtcacggc
      601 gtagccatat ttgaccggga acggaaaagc gccctcctgc atgggtgcgc gcatgcgctc
      661 ccattcgctc ggcccgatgg cacccagcaa aaccagccgt tccgtgccgc ggctgatgcc
      721 gctaaagagg ctgcggacgc gggcatgccc cggggcgggg ggtggcagtg gcgcggtgcg
      781 cagttccacg ctgcccttct ttacgtacca gagcgcccgt gccatgcggg tcttgccggt
      841 tgctcggcgc gccatcatgt taccgctccg aattgccagg attgcgccgc tgccataaac
      901 cacccggatg aacactagat gagcgacagc ttcgatgccc ccgcagcggg ggcactgcca
      961 gcgccagcgc tgagccttac gacacccacc ggcctccagt ccatcgatga gctggcgcgg
     1021 cgtcggcgca tcgtcttggg cctcaacgtc atcacctatg gcctgatgct gtggtgggcc
     1081 ggccgcattc tcgccgccgg cggttggacc tgggtcgacg gcgtgctgtt tgcctgtttt
     1141 gcgctcggta cgccgtgggc cgtgctcggc ttttggaacg cgctgatcgg tctgtggctt
     1201 ctcaacgttg ctggcaagca cgccggcatg gccgtggcgc cgtacgcggc ggctggtgct
     1261 gagccaacgc cgatccgcat caagaccgcc atcttcatga ccttgcgcaa cgaggacgcc
     1321 gcgcgcgcca ttcagcgctt ccaaatcgtg caagatagcg tcgagcggac cggcgagggt
     1381 gccgccttca gctatttcgt cctgagcgac agcaacgacg gcgccgtggc cgcggccgag
     1441 gaggcggccg tcgccgcctg gcaggccgtc gccggcgccc cagatcgcat cacctatcgc
     1501 cggcgcagca gcaatgtcgg ctacaaggcc ggcaacgtgc gcgacttctg cgcccgctgg
     1561 ggcgatgact tcgacctcat gctgccgctc gatgccgaca gcttgatgtc gggcgcccag
     1621 atcgtgcgct tggtgcgcat gatgcaggcg catcccaaga ttggcatctt gcaaagcctt
     1681 gtggtcggca tgccctctgc cagcgctttc gcccgcattt tccagttcgg catgcgccac
     1741 ggcatgcggg cctacaccat gggccaggcc tggtgggtcg gcgactgcgg acccttttgg
     1801 gggcacaatg cgctggtgcg cgtgagaccg ttttatgccg aatgcgacct gcctctttta
     1861 cctggcaagc cgccactcgg cggccacgtc ctgtcgcacg atcaggtgga agcgaccctg
     1921 atgcgccggg ccggctacga agtgcgggtg ttgcccgagg aggttggcag ctgggaagaa
     1981 aatccaccga ccatgctgga gtttgcccgc cgcgacgtgc gctggtgtca gggcaacatg
     2041 cagtatctga agctgctcga cctcccgggc ctttatccca tgagccggtt ccagctgctg
     2101 tgggcgattc tgatgttcct cggcatcccg gcctggacgc tgatgatcgc cctcctgccg
     2161 ctggccgcct ggcaggctca atcggttccc gagttcccaa cgggcgccgc caaggctctc
     2221 tatgcgacct actttatcat gtacttgatg cccaagatcg ctgggctcct cgatgctatg
     2281 ttcggccgcg gtggtgtggc gcgctacggc ggctggcttc ggtttctcat cagcgcggtg
     2341 ctcgagctca cgttctcgtt cctgcagggc gccgtctcca ccatccgcac ctccatcttc
     2401 atgctgggcc tactgttcgg caggtcggtg gtttggggtg ggcaggtgcg cgacgcccag
     2461 cgcctgtcgt ggagtgaagg cgcgcgccac ctgtggccgc aaaccttgtt tggcgtgatc
     2521 gtgtgtggcg cgctggccgt gatcgcgccg gcggtgctgt ggtggagcct gccgctcacc
     2581 gccggttatc tgttggccat tccctttgcc gtcatcacgg cggatccggc gttcggtcgc
     2641 gccctcaagc gttttggcat ttgcggcatt ccggaggact tcaggccgcc agaggaaatc
     2701 gtcgccaccc gcgcgacgac aacctgagag cttgcccatg gccattgagg atcgcgtcgg
     2761 cgctctcagc aatgatccgg cattcgccac actgcaccgc tcgcttgccg tctactacgg
     2821 ccacgccgca cgcgatgccg ccatggatcg gctctatcgg cgcttcctcg gcctgggcga
     2881 gcttgccttc gacattggca gccatgtcgg tgaccgtgtc ggcagctttc gtcgccttgg
     2941 tgctcgcgtg gtcgcgctcg agccgcagcc gttgtgtgca cgcgcgatcc gaaccattta
     3001 cgccgacgat aacgacgtg
//
LOCUS       DASTMT010000106         1907 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_1367438, whole genome shotgun sequence.
ACCESSION   DASTMT010000106 DASTMT010000000
VERSION     DASTMT010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 1907)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1907)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1907
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1367438"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..258
                     /locus_tag="VFR00_02220"
     CDS             <1..258
                     /locus_tag="VFR00_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019171581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0158094.1"
                     /translation="APDLLLLDEWLAGLNPTELEAGIELVRSLEGDGLTIVMVEHVMY
                     AIRSLCQQVVVLNAGRVIASGAPEEALADPDVIRVYLGDGV"
     gene            585..1085
                     /locus_tag="VFR00_02225"
     CDS             585..1085
                     /locus_tag="VFR00_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018261277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEU0158095.1"
                     /translation="MVKLSMTVNGKPVTAEVDARTLLVQFLRENLRLTGTHVGCDTSQ
                     CGACVVHLDGRAVKSCTTLAASCEGAKVVTIEGLAQGGKLHPMQEAFREHHGLQCGFC
                     TPGMIMAAVDIVARRGARVSEEVVRADLEGNICRCTGYHNIVAAVLAGASAMAASGGA
                     KPQAAE"
     gene            1147..>1907
                     /locus_tag="VFR00_02230"
     CDS             1147..>1907
                     /locus_tag="VFR00_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014439132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEU0158096.1"
                     /translation="MSATGIGASVKRKEDQRFITGKGQYTDDINRPGQAYAVFIRSPH
                     AHANLKKVDLSAALKSPGCLGAFTGADLAADKVGTLICGWMIHSKDGSPMKAGAHPAL
                     AQGKVRYVGDHVAVVIGETLNDAKDAAEKVNVEYETLPAITDLAAAQKAGQPQIHAEA
                     PNNTVYQWHLGDKAAVDAAFASAKHVTQIDLINNRLVPNAIEPRAAIGEYDAGNDSLT
                     LYTTSQNPHVTRLVLAAFIGLAPEHKLRVIAPDVGG"
BASE COUNT          356 a          638 c          627 g          286 t
ORIGIN      
        1 gccccggacc ttctcttgct cgatgaatgg ctggcggggc tcaatccgac cgaactcgag
       61 gcgggcatcg agcttgtgcg ttcgctcgag ggggacgggc tcaccatcgt catggtcgag
      121 cacgtgatgt acgcgatccg ctccttgtgc caacaggtgg tggtgctcaa tgccggtcgc
      181 gtgatcgcca gcggcgcgcc ggaagaggcg ctggccgatc ccgacgtcat ccgcgtctat
      241 ctgggcgatg gcgtctgagg tcttctgccg gggtagtggc ttgcgcatgc gcagcccagc
      301 gcgcaacggg caaatggccg cgggccgggc tatgccacgg cggcgccgcc ccagccgtgc
      361 aggcgccgac gcgattgcgc aaagcgctga gctgtggcgt aattgagcgc gaggacaagc
      421 aagaatagtc agcgtccggc caagccttgg cgccagagag gaggaagtcc atgttttctc
      481 tgtttcgcaa ggacaagaaa ccggccgaac cggcggcagc atcgcccacg gccgcaccgg
      541 gggccgccgc tggccgcgcc acgggaacgt caggaggggg acgcatggtc aagctcagca
      601 tgacggtgaa cggcaagccg gtcaccgccg aggtcgacgc caggacgctg ctcgtgcaat
      661 tcctgcgcga gaacttgcgc ctgaccggca cccacgtcgg ctgcgacacc agccagtgtg
      721 gtgcttgcgt ggtccatctc gatgggcgtg cggtcaaatc gtgcaccacg ctggccgcat
      781 cctgcgaggg cgccaaggtc gtgaccatcg aaggcttggc gcagggcggt aagctgcatc
      841 cgatgcagga ggccttccgc gagcatcatg gcctgcaatg cggcttctgc acgcccggca
      901 tgatcatggc cgccgtcgac atcgtcgccc gacggggcgc ccgggtgagc gaggaggtgg
      961 tgcgcgcgga tctcgagggc aacatttgcc gctgtactgg ctatcacaac atcgtcgccg
     1021 ccgtgctcgc cggcgcctcg gcgatggccg cctcgggcgg cgccaaaccg caagccgccg
     1081 aataacgccc ataacgccat ccaaccggcg ctggcatggc cgcgccgacg ctcaggggag
     1141 aagacgatga gtgccacagg catcggcgcg tccgtcaagc gcaaggaaga tcagcggttc
     1201 atcaccggca agggccaata taccgatgac atcaatcggc cgggccaagc ctatgccgtg
     1261 tttatccgct cgccgcatgc gcacgcgaac ctgaagaagg tcgacctctc ggccgccctg
     1321 aaatcgccgg gctgcctcgg cgccttcacg ggagcggacc tggcagccga caaagtcggc
     1381 accctgattt gtggctggat gatccactcc aaggatggtt cgcccatgaa ggcgggcgcg
     1441 catccggcgc ttgcccaggg caaggtgcgc tacgtcggcg accacgtggc cgtcgtcatt
     1501 ggcgagacgc tgaacgacgc caaggatgcc gccgaaaagg tgaacgtcga atatgagacg
     1561 ttgccggcga tcaccgacct tgccgccgcg cagaaggctg gccaaccgca aatccacgcc
     1621 gaggccccca ataacaccgt ctatcagtgg cacctgggcg acaaggccgc cgtcgacgca
     1681 gcctttgcca gcgccaagca cgtgacccag atcgacctca ttaacaatcg gctggtgccc
     1741 aacgccatcg agccgcgcgc ggccatcggc gaatacgacg ccggcaacga cagcctcacg
     1801 ctctacacca ccagccagaa cccgcacgtc acgcgcctgg tgctcgccgc cttcatcggc
     1861 ctcgctcccg aacacaagct gcgcgtgatt gcgccagacg tcggggg
//
LOCUS       DASTMT010000107         2974 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_3336499, whole genome shotgun sequence.
ACCESSION   DASTMT010000107 DASTMT010000000
VERSION     DASTMT010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2974)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2974)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2974
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3336499"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            50..1087
                     /gene="mepA"
                     /locus_tag="VFR00_02235"
     CDS             50..1087
                     /gene="mepA"
                     /locus_tag="VFR00_02235"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019199629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HEU0158097.1"
                     /translation="MRACLALCGLIVIAASPLLLAAEPAPQAEATQETAKSITGKTPV
                     PKKPRAKHATPASPPAKQLFGAIKEPASMPARSIGFYAKGCLAGAAALPIDGPAWQAM
                     RLSRNRNWGHPDLVALVEKLANEARAHDGWPGLLVGDLSQPRGGPMLSGHASHQVGLD
                     ADIWLTPMPNRRLSEKEREEIAATSMLAENKISVDPKVWTDAHVKLLKRAASYATVER
                     VLVHPAIKKALCEATAKDKDRAWLHKVRPIWGHYYHFHVRIACPKGSTNCEAQPEPKS
                     DDGCGKELTDWIALLQKPPGPPGPPTPPKPALTLEQLPAACKAVLASGTNTAAPGTTT
                     SASKPTTSKTK"
     gene            complement(1224..>2974)
                     /locus_tag="VFR00_02240"
     CDS             complement(1224..>2974)
                     /locus_tag="VFR00_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEU0158098.1"
                     /translation="DAAVVVCEPDPKRVPALQLILKQLEDRGIPRFLFLNKIDSFEAQ
                     VRDILPILQPASSKPLVLRQIPIWENGAASGFVDLALERAFVYRAQANAEVIEIPAGV
                     KERENDARFKMLEQLADYDDELMEQLLSDVQPTREKVFADLVKEVQEGLIVPVLLGSA
                     ENGNGILRLLKALRHEAPFVERTAQRLKLENAKSAGQVLKTMYTAHGGKISLVRVLAG
                     EFGEGTIAQGARAEERAASAFSLLGQEAKKRGAGKPGDTLALGRLEKILTGETISADK
                     GGITQVKAPEPPEAVFGVAIGVKDRKDEVKLTGALGRLMEEDPSLKLQHAKDTHQMVL
                     WGQGEMHLRVALERLKRKYGVEAESKPRQVPYKETIRRPVEIRGRHKKQSGGHGQFGD
                     VVLDIKPLPRGTGFQFAEKITGGVVPRQFIPSVEIGVSDYLQRGPLGGFPVVDVSVTL
                     IDGSYHSVDSSDMAFRQAGRIGMAEGMPKCSPVLLEPIMEVEIAIPNEATARINGMIP
                     QRRGQILGFDAREGWPGWDVVRAQIPESEMGDLIVELRSATAGVGTFSAKFHHLAELT
                     GRLADNVLAAHKEAAE"
BASE COUNT          523 a         1009 c          944 g          498 t
ORIGIN      
        1 cgttgggcga gattgccgag ccgggaagcc aagcgacgga gagcgcggaa tgcgagcatg
       61 ccttgcgctg tgcggcctca tcgtcatcgc cgcaagccca ctgctgttgg ccgccgagcc
      121 ggcgccgcag gcggaggcta ctcaagagac tgccaagagc ataaccggta agacgccggt
      181 gccgaaaaag ccgcgcgcaa aacacgcaac acccgcctcg cccccggcca agcagctgtt
      241 cggcgctatc aaggagccgg cgtcgatgcc ggcgcgctcg atcggctttt atgccaaggg
      301 atgtctcgcc ggcgccgccg ccctgccgat cgacgggccg gcctggcagg cgatgcggct
      361 ctcgcggaat cgcaactggg gacatcccga cctcgtggcg ctggtcgaga agcttgccaa
      421 cgaggcgcgc gcccatgacg gctggccggg gctgctggtc ggggacctgt cgcagccgcg
      481 cggcgggccg atgttgtcgg gccatgccag ccatcaggtc ggcttggatg cggacatctg
      541 gctgacgccc atgcccaacc ggcgcttgag cgaaaaggag cgcgaggaga tcgccgccac
      601 ctcgatgctc gccgagaata agatctcggt cgatcccaag gtgtggacgg acgcgcacgt
      661 taagctgtta aaacgggctg catcctacgc cacggtcgag cgcgtgctgg tgcaccctgc
      721 catcaagaag gcgctgtgcg aggcaaccgc caaggacaag gatcgcgcct ggctgcacaa
      781 ggtgcgcccg atctggggtc actactatca tttccacgtc cgcatcgcct gtcccaaggg
      841 cagcaccaat tgtgaagccc agcccgaacc gaaaagcgac gacggctgcg gcaaagagct
      901 gaccgactgg atcgcgttgc tgcagaaacc tcctggcccc ccgggcccgc cgacgccgcc
      961 aaagccggcg ctgacgctgg agcaactgcc ggccgcctgc aaagcggtgc tggccagcgg
     1021 caccaacacg gccgccccgg gcacgaccac ttcggcaagc aagccaacga ccagtaagac
     1081 gaagtagccg ccgccgctgt gcaaccgcgg tgttgcgcgc gcgaaggaac gcgcgcgcgc
     1141 tttttctttg cacccgtagc ggatcttgcg ccgcagggca gagccacgcc cgccagcgtg
     1201 gctctgcgag ttcgtctcga ggactactcg gccgcttcct tgtgggctgc cagcacgttg
     1261 tcggcgagcc gccccgtgag ctcggccaag tggtggaact ttgccgagaa ggtgccaacg
     1321 ccggccgtcg ccgagcgcag ttcgacgatc agatccccca tctcagattc ggggatctgg
     1381 gcccgcacca cgtcccaccc gggccagccc tcgcgcgcgt cgaagccgag gatctgccca
     1441 cggcgctgcg gaatcatgcc gttgatgcgc gcggtggcct cgttcgggat tgcgatctcc
     1501 acctccatga tcggctccag caagacggga ctgcatttcg gcatgccttc cgccatgccg
     1561 atgcgacccg cttgccggaa cgccatgtcg gaggaatcga cggagtgata ggagccgtcg
     1621 atcagcgtga ccgaaacatc caccaccggg aagccgccga gcggcccgcg ttgcaagtag
     1681 tcggatacgc cgatttcaac ggagggaatg aactggcgcg gcaccacgcc gccggtgatc
     1741 ttttcggcaa actggaaccc tgtaccgcgc ggcaaaggct tgatatccaa gacgacgtcg
     1801 ccgaactggc cgtgcccgcc cgactgcttt ttgtgtcgtc cgcggatttc gaccggccgt
     1861 cggatcgttt ccttgtaggg gacctggcgg ggcttggatt ccgcctccac gccatacttg
     1921 cgtttgagac gttcgagcgc cacgcgcagg tgcatttcgc cctgccccca cagcaccatt
     1981 tggtgcgtgt ccttggcgtg ctgcagcttg agcgaggggt cctcctccat cagccgaccg
     2041 agcgcgccgg tcagcttcac ctcgtctttg cggtccttga cgccgatggc gacgccgaac
     2101 accgcctcgg gtggctcggg cgccttcacc tgggtgatgc cgcccttgtc cgcggaaatg
     2161 gtttccccgg taaggatctt ctccaggcgg ccgagcgcca gcgtatcccc gggcttgccc
     2221 gcaccgcgct ttttcgcctc ctggcccagc agggaaaagg cagaggcagc gcgctcttcg
     2281 gcgcgcgcac cctgtgcgat cgtgccttcg ccgaactcac cggccaacac gcgcacgagc
     2341 gagatcttgc cgccatgcgc tgtatacatg gttttgagca cctgcccggc ggacttggcg
     2401 ttctccagct tcagccgctg ggccgtacgc tcgacaaaag gcgcctcgtg ccgcagagcc
     2461 ttgagcagcc gcaagatgcc attgccgttc tcggccgagc cgagcagcac gggcacgatc
     2521 aagccctcct gcacctcctt gaccagatcg gcgaaaacct tttcacgcgt cggctgcacg
     2581 tcggacaaca gctgctccat cagctcgtcg tcgtagtcgg caagctgttc gagcattttg
     2641 aagcgtgcat cgttctcgcg ctctttcacg ccggcgggaa tttcgatcac ctcggcattg
     2701 gcctgcgcgc ggtacacaaa ggctcgttcc agcgccagat cgacaaagcc ggacgccgca
     2761 ccgttctccc agatcgggat ctgccgcagc accaatggct tggagctcgc gggctgcagg
     2821 atcggcagga tgtcgcgcac ttgcgcttcg aacgaatcga tcttgttcag aaacagaaag
     2881 cgggggatgc cgcggtcctc aagctgcttg aggatcagct gcagggcggg aacgcgcttc
     2941 gggtcgggct cgcacacgac gacggccgca tcgc
//
LOCUS       DASTMT010000108         2009 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_712649, whole genome shotgun sequence.
ACCESSION   DASTMT010000108 DASTMT010000000
VERSION     DASTMT010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2009
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_712649"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2009)
                     /gene="putA"
                     /locus_tag="VFR00_02245"
     CDS             complement(<1..>2009)
                     /gene="putA"
                     /locus_tag="VFR00_02245"
                     /EC_number="1.2.1.88"
                     /EC_number="1.5.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0004657 - proline dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010133 - proline catabolic process to
                     glutamate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional proline dehydrogenase/L-glutamate
                     gamma-semialdehyde dehydrogenase PutA"
                     /protein_id="HEU0158099.1"
                     /translation="SPRHSAALFERPSLSVKLSAIHPRFDPGKEDRLGRELLPRLIEL
                     ARAARRHELTLTIDAEEQDRLDLTLDQFAAAFAHPALDGWPGLGIAVQAYGKRAIPVL
                     RWLRRLAEQRRKRIPVRLVKGAYWDSEIKWAQERGLADYPVFTRKLATDVSYLAAMRL
                     LLADEAAFFPQFATHNAQSMASVAVAAGERTFEFQRLHGMGEALYEDMVGRDRGLACR
                     IYAPVGAHADLVPYLVRRLLENGANTSFVNRLADDALPVEAIVADPVETAEHERERQV
                     RLMPRPPEIFAPRLNSQGLALYEPATRAALYRQLETALAASYAAAPIVAGEVVGGREA
                     SVVLCPHDRRQRIGTVATTDRATIDAAVADAERAAVAWDLAGGAARAAALERAADLYE
                     RNRAQLMAVIVREAGKTLENALGDVREAIDFLRYYASEARRLFTTPMALPGVTGESNL
                     ITLRARGPFACISPWNFPLAIFTGQVAAALAAGNPVLAKPAEQTPIVAFLATQLLHEA
                     GIPPEALQLLPGGGEVGAALIKDQRVAGVAFTGSNDTGWAIQTALAARRGAMVPFIAE
                     TGGINAMIADSSALPEQVIRDLVRSAFDSAGQRCSAARLFFVQEDVARVMIDMLVGAV
                     EALDIGDPLHFATDIGPVIDDDAVARLEAHKLRLQRQAHQLVDLPL"
BASE COUNT          354 a          672 c          681 g          302 t
ORIGIN      
        1 agggggagat cgacgagctg gtgcgcctgt ctttgcaggc gcagcttgtg ggcctcgagg
       61 cgcgcgacgg catcgtcgtc gatcaccggc ccaatgtcgg tggcaaagtg gagcggatcg
      121 ccgatatcga gcgcttccac cgcccccacc aacatatcga tcatcaccct ggccacgtct
      181 tcctgcacaa agaacaggcg cgcagccgag cagcgctggc ccgccgaatc gaaggccgag
      241 cgcaccagat cgcggatcac ctgctcgggc aaggcactcg agtcggcgat catggcgttg
      301 atgccgccgg tctcagcaat aaagggaacc atggcgccgc ggcgcgctgc cagcgccgtc
      361 tggatcgccc agcccgtgtc gttggagccg gtgaaggcga caccggctac gcgttgatcc
      421 ttgatgaggg cggcaccaac ctcgccgccg cccggcagca gctgcagcgc ctcgggcggt
      481 atccccgcct catgcaaaag ctgcgtggcg agaaaggcaa cgattggcgt ttgctccgcc
      541 ggcttggcaa gcacgggatt gcccgccgcc aaagcagccg ccacctgccc ggtaaagatc
      601 gccagcggga aattccatgg gctgatgcag gcgaacgggc cgcgagcccg caacgttatg
      661 agattgctct cgcccgtcac gcccggcagc gccatcggtg tcgtgaacaa gcgtcgggct
      721 tcgctggcat agtagcgcag gaaatcgatg gcctcgcgca catcgcccag ggcgttctcg
      781 agcgtcttgc cggcctcacg cacgataacg gccatcagtt gggcgcggtt gcgctcataa
      841 aggtcggcgg cgcgttccaa ggcggcggcg cgtgccgcgc cgccagccag gtcccaagcc
      901 accgccgcgc gctcggcgtc ggcgacggcg gcatcgatcg tggcccgatc ggtggtcgcg
      961 accgtgccga tgcgctggcg ccggtcgtgt gggcacagca cgaccgaggc ttccctgccg
     1021 ccgacaacct cgcccgcgac gattggcgct gccgcgtagg acgccgccag cgccgtctcc
     1081 agctgccgat aaagggcggc gcgcgttgcc ggctcgtaca gcgccagacc ctgactgttg
     1141 aggcgcgggg caaagatctc gggcggtcgc ggcatcaacc gaacctggcg ctcgcgttca
     1201 tgctcggccg tctcgacggg atcggcaaca atggcctcga ccggcaaagc atcgtcggcc
     1261 aggcgattga cgaaagaggt attggcaccg ttctccagca gccgacgcac aagatagggt
     1321 acgaggtctg cgtgtgcgcc gaccggggca tagatgcggc aggcgaggcc ccggtcgcgg
     1381 ccgaccatgt cctcataaag cgcctcgccc atgccgtgca gtcgctgaaa ctcgaacgtc
     1441 cgctcgccgg cggcgaccgc gacgctcgcc atcgactggg cattatgcgt ggcaaactgc
     1501 gggaaaaagg cggcctcatc cgccagcaac agccgcatgg cggcgagata ggatacatcg
     1561 gtggcgagct tgcgcgtgaa caccgggtag tcggcaagtc cacgctcctg cgcccacttg
     1621 atctcgctgt cccaataggc gcctttgacc aggcgcacgg gaatgcgctt gcgtcgttgc
     1681 tcggccagcc gtcgcagcca gcgcagaaca ggaatggcac gcttgccata ggcctgcacg
     1741 gcgatgccga gaccgggcca gccgtccagg gcggggtgcg cgaaggcggc ggcgaactgg
     1801 tcgagtgtca ggtcgagccg atcctgttcc tcagcgtcga tggtcaaggt aagctcgtgc
     1861 cgccgggcgg ccctggccaa ttcgatgaga cgcggcagca attcccgccc gagacgatcc
     1921 tctttccccg gatcgaagcg cggatgaatg gccgacagct tgaccgagag gctcgggcgc
     1981 tcgaacagcg cggcgctgtg ccgggggct
//
LOCUS       DASTMT010000109         8779 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_57937, whole genome shotgun sequence.
ACCESSION   DASTMT010000109 DASTMT010000000
VERSION     DASTMT010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 8779)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8779)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8779
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57937"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..556)
                     /locus_tag="VFR00_02250"
     CDS             complement(<1..556)
                     /locus_tag="VFR00_02250"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HEU0158100.1"
                     /translation="MTSPYTLVAYAGWVLILLVWIACTFKTRKAVRHTKPLQQVAATS
                     LIALAFVLLFQRRASPLLGMQLGEAPAPVALVAAVVCLAGVGFAIWARLTLGRNWSGA
                     LASVGENHELVVEGPYRYVRHPIYAGFLIAMAATAITSGTLTSYLAVLVGLLAFLMRM
                     AIEEALMLSQFPEAYRAYQAKTAAL"
     gene            754..1626
                     /locus_tag="VFR00_02255"
     CDS             754..1626
                     /locus_tag="VFR00_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012110922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HEU0158101.1"
                     /translation="MAAYRRIEVRPIAGALGAEICGVDLGALEAETFAEIHAAWLEHL
                     VVFFRNQSLAPPQQIAFAKRFGEIHHHPFMQGMEAYPDILEIVKEEGDSKAFGEVWHT
                     DQMFNPKPAKATILYAKETPDAGGDTLFANMYLAYETLSEPMKALIKELRTFNIGDRK
                     KLGRSHEVTATRDGRYTGNAKMAAKVREPGDLVTEASHPLVRTHPETGRKALYLSNHT
                     QTLDGFRPGEARPILDYLAQHAVEPEFTCRFRWEVGSLAIWDNRCTQHRALNDYPGKR
                     RRMHRITIAGDAPY"
     gene            complement(1767..5567)
                     /locus_tag="VFR00_02260"
     CDS             complement(1767..5567)
                     /locus_tag="VFR00_02260"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HEU0158102.1"
                     /translation="MHIKRRFTTEGKSPYATIGFRRATSEIKNPDGSVVFKLENFAVP
                     EHWSQVAADILAQKYFRKAGVPMRLKRIEETQVPSWLWRSVPDERALSERAGHERTTG
                     ETDARQVFDRLAGTWTYWGWKGGYFTNEDDARAFFDEHRYMLAMQMGAPNSPQWFNTG
                     LHWAYGIDGPGQGHFYVDHETGELVASTSAYEHPQPHACFIQSIADDLVNEGGIMDLW
                     VREARLFKYGSGTGSNFSNLRAENEKLSGGGKSSGLMSFLKIGDRAAGAIKSGGTTRR
                     AAKMVVVDIDHPDIEAYIDWKVKEEQKVAALVTGSKLCQRRLKALLKACVNCEAEGEA
                     CFDPAKNPALKRAIKEARRDLVPDPYIRRIIQFARQGYKDLEFASYDTDWDSEAYLTV
                     SGQNSNNSVRVTDEFLNAMLEDRDWQLTARLSGKPVKSVKARELWEKIGYAAWASADP
                     GIQFHTTINDWHTCPKSGPIRASNPCSEYMFLDDTACNLASLNLMMFRGPDKRFDVAA
                     YEHACRLWTIALEISVMMAQFPSRRIAELSYRYRTLGLGFANIGGCLMASGIPYDSAE
                     GRAICGALAAIMTGVAYATSAEMASELGTFPGYEANRADMLRVVRNHRRAAYGQRDGY
                     EKLAIPPVPLDHVACPDPHLTEAAQRAWDRAIELGQQHGYRNAQATVIAPTGTIGLVM
                     DCDTTGIEPDFALVKFKKLAGGGYFKIINQAVPEALRTLGYGEAQIAEIEAYAVGHGS
                     LAQAPAINHASLKARGFSSEVLTKLDAALRSAFDIKFAFNRWTIGEEFLTRTLQIEAE
                     KLSEPGFDLLGHLGFSKKDIEAANEHVCGAMTLEGAPHIGKEHLPVFDCASPCGRKGR
                     RFLSVDSHIHMMAAAQPFVSGAISKTINMPNDATVEDCKTAYMLSWRLALKANALYRD
                     GSKLSQPLNAQLLADEAEEAEEVIGEIGADKPMATRAAVTAERIVERIVERERQREKL
                     PNRRKSYTQKATVGGHKVYLHTGEYEDGRLGEIFIDMHKEGAAFRSLMNNFAIAISLG
                     LQYGVPLEEYVDAFTFTRFEPAGLVQGNDTIKNATSILDYIFRELAVSYLGRNDLAHV
                     DLSEVANTGLGSSEQDLEEDEAPPPAAKYLSRGLLRGQESRVLALRSTSSVAVAEEAE
                     AASPVAALQQGVSSAFTAPKQRMEAAVEHLAAVHQELAASAPAPRAEKLSVAELAAQL
                     SEQLAQRAAARAGRSSSAVERAAEARVKGYEGESCRECGNFTLVRNGTCLKCDTCGGT
                     SGCS"
     gene            6165..6563
                     /locus_tag="VFR00_02265"
     CDS             6165..6563
                     /locus_tag="VFR00_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013948400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0010257 - NADH dehydrogenase complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone oxidoreductase subunit NDUFA12"
                     /protein_id="HEU0158103.1"
                     /translation="MGIFSEIFSWWGGNTWSNRLYTAWRGSYVGSDAAGNRYYVQRRG
                     VGPLGVPRRWVIYQHGAEASQVPPEWHGWMHYMVDTPPSEESYTPHPWEKPHRMNMTG
                     TAEAYRPSGSILKSGRRPKATGDYKPWQPN"
     gene            6647..7159
                     /locus_tag="VFR00_02270"
     CDS             6647..7159
                     /locus_tag="VFR00_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2155 domain-containing protein"
                     /protein_id="HEU0158104.1"
                     /translation="MRRDGVRQDLRRERKAAAAAALTVLLLALPAAAQRIDNAVAIFA
                     ALDKVTAKISRLEVPLNQTAKFGALKITPRVCYSRAPTEPPKTTSFVEVDETQLDGKE
                     KRIFSGWMFADSPGLNAVEHPVFDVWLTDCGQPQRVSGPRPPPGAGPAASEPPPPSEQ
                     DLPRRRRVPR"
     gene            complement(7177..7854)
                     /gene="aat"
                     /locus_tag="VFR00_02275"
     CDS             complement(7177..7854)
                     /gene="aat"
                     /locus_tag="VFR00_02275"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008189264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HEU0158105.1"
                     /translation="MASRDDIVIEITPQVLLKAYACGIFPMAESCDDPALYWIEPQQR
                     GILPLGAVHVPRRLARTVRQASYEIKIDHDFEAVIDGCALSRPGRRTTWINRRIRGLY
                     RDLFDAGYCHTVEAWAGARLLGGLYGVALNGAFFGESMFSNARDASKVALVYLCARLI
                     AGGFSLLDTQFVTEHLRQFGTVELDRNAFHALLEKALAHQADFAALPTATAPEEILAI
                     IAGGNAR"
     gene            complement(7890..>8779)
                     /locus_tag="VFR00_02280"
     CDS             complement(7890..>8779)
                     /locus_tag="VFR00_02280"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018325915.1"
                     /note="an AccC homodimer forms the biotin carboxylase
                     subunit of the acetyl CoA carboxylase, an enzyme that
                     catalyzes the formation of malonyl-CoA, which in turn
                     controls the rate of fatty acid metabolism; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEU0158106.1"
                     /translation="GFPVLVKAAAGGGGRGMKVARGPEDFELALRTARSEAKAAFGDD
                     SVYLEKYLAKPRHIEFQVFGDGKGNAIHLGERDCSLQRRHQKILEEAPSPALNAEQRQ
                     GMGEVVAKAMQKLKYRGAGTVEFLYEDGEFYFIEMNTRLQVEHPVTEMITGLDLVIEQ
                     IRIASGAPLSISQSEFALSGHAIECRINAENPKTFMPSPGTITYWHPPGGLGVRVDSG
                     AYQGYRIPPYYDSLIGKLIVHGKNRNECLMRLKRCLSEFVIDGIETTIPLFTELVQNS
                     DIANGLYDIHWLESFLQKP"
BASE COUNT         1510 a         2782 c         2941 g         1546 t
ORIGIN      
        1 cgagggctgc ggttttcgcc tgataggccc gataggcctc cggaaactgc gacagcatca
       61 gggcctcctc gatggccatg cgcatcagga aggcgaggag ccccaccagc accgccaggt
      121 agctcgtcag cgtgccgctg gtgatcgcgg tcgccgccat ggcgatcagg aaaccggcgt
      181 agatggggtg gcgaacgtaa cgatagggcc cctcgaccac gagctcatga ttttcgccga
      241 ccgaggccag cgcgcccgac cagttgcgcc cgagcgtcag ccgcgcccaa atcgcaaatc
      301 ccacgcccgc caggcaaacc acagcggcca cgagcgcgac cggcgcgggg gcctcgccca
      361 gctgcatacc caatagcggg ctcgcgcggc gctggaataa caagacgaag gccaaggcaa
      421 tgagcgacgt ggccgccacc tgctgcagcg gcttggtatg ccgcacggcc ttcctggtct
      481 tgaaagtgca ggcgatccaa accaggagga tgaggaccca gccggcgtag gccacgagcg
      541 tgtagggtga ggtcatgggc caactcgtgt tggcaggcgc ttcgccccgg agttggcgcg
      601 caatcctggg cactgttggg gcaaggggca acctgccgcg ccgaatagaa ggggggtggt
      661 tgcgtgcacg ctctgcgcgc ttgcggttga cccaggtggc gcatctttgc cacaattcgg
      721 cgcagtggcc gtgcgggagt gaggggtcag atcatggcag cctatcgtcg cattgaggtg
      781 aggcccatcg ccggggcgct cggcgcggag atctgtggcg tcgatctcgg cgcgctcgag
      841 gcagagacat ttgcggaaat ccacgccgcc tggctcgagc atcttgtggt gtttttccgc
      901 aaccagagcc ttgcgccgcc ccagcagatc gccttcgcca agcgcttcgg cgaaatccat
      961 catcacccct tcatgcaagg catggaggcc taccccgaca tcctcgaaat cgtcaaagag
     1021 gaaggtgaca gcaaggcctt cggcgaagtc tggcacaccg accagatgtt caatcccaag
     1081 cccgccaagg ccaccattct ctacgctaag gagacgccgg atgcgggcgg ggatacgctc
     1141 tttgccaaca tgtatctggc ctacgaaacc ctatccgagc cgatgaaggc gttgatcaag
     1201 gagctgcgca ccttcaacat cggtgatcgc aaaaagctcg ggcgtagcca cgaagtgacg
     1261 gcaacgcgtg atggccgcta tacgggcaat gccaagatgg cggccaaggt gagggagccg
     1321 ggtgacctcg tcaccgaggc cagtcacccc ctcgtgcgca cccaccccga gacgggccgc
     1381 aaggcgctct atctcagcaa ccacacgcaa acgcttgacg gcttcaggcc gggcgaggcg
     1441 cgaccgatcc tcgactatct ggcgcagcat gccgtcgagc cggagttcac ctgccggttc
     1501 cgctgggagg tcggctcgct cgccatttgg gacaaccgct gcacgcagca tcgggcgctg
     1561 aatgactatc ccggcaagcg gcggcgcatg caccgcatca ccatagctgg cgacgccccg
     1621 tactagacag cgccgccctg cggcgagacg atcctcgccg cagtcactgg ggggccgcgc
     1681 agccagctgc gctacccacc ttcggaataa acttgagagg ggtgattatt tctccggcgg
     1741 gttcggtagt cctcaagtcg gaataactaa gagcacccgg atgtacctcc gcaggtatcg
     1801 catttgagac aggtgccgtt gcgcaccagc gtgaagttgc cgcattcgcg acagctctcg
     1861 ccctcatagc ccttgacccg ggcctcggcg gcgcgctcga ccgcgctgct gctgcggccc
     1921 gcgcgcgccg ccgcgcgttg ggcgagttgc tcgctcagct gggcggccag ctcggcgacg
     1981 ctgagctttt cggcgcgcgg cgccggcgcg ctggcggcca gctcctggtg cacggcggcc
     2041 aggtgctcaa cggctgcctc catgcgctgc ttgggggcgg taaaggcgct gctcacccct
     2101 tgctgtaacg ccgccaccgg gcttgccgcc tcggcctctt cggcgaccgc cacgctgctg
     2161 gtggagcgca gcgccagcac ccggctctcc tgaccgcgca agaggccgcg cgaaagatac
     2221 ttggccgccg gcggcggggc ctcgtcctcc tccagatcct gctcggagga gccaagtccc
     2281 gtgttggcga cctcgctcag gtcgacgtgg gccagatcgt tgcggccgag gtaggagacc
     2341 gccagttcgc ggaaaatgta gtcgagaatg gacgtggcgt tcttgatggt gtcgttgccc
     2401 tgcaccagcc ccgccggctc gaagcgcgtg aaggtgaagg catcgacgta ttcctcgagc
     2461 ggcaccccgt actgcaggcc aagcgagatg gcgatggcga aattgttcat caacgagcgg
     2521 aaggcggcgc cttccttgtg catgtcgatg aagatttcgc ccagccgtcc gtcctcgtac
     2581 tcgcccgtat gcagatacac cttgtggccg ccgacggtgg ctttttgcgt atagctcttg
     2641 cggcggttgg gaagcttctc gcgctggcgt tcccgttcga cgatgcgctc gacgatgcgc
     2701 tcggcggtga ccgcggcgcg cgtcgccatc ggcttgtccg cgccgatctc gccgatcacc
     2761 tcctcggctt cctcggcctc gtcggccagc agttgcgcgt tgagcggctg cgacagcttg
     2821 gagccgtcgc gataaagcgc attggccttg agcgccagcc gccaggacag catgtaggcc
     2881 gtcttgcaat cctcgaccgt ggcgtcgttg ggcatattga tggtcttgga gatggcgccg
     2941 gagacgaagg gttgcgccgc cgccatcatg tggatgtggc tgtcgaccga caggaagcgc
     3001 ctgcccttgc ggccgcaggg actggcgcaa tcgaagacgg gcaggtgctc cttgccgata
     3061 tgcggggcgc cttccagcgt catggccccg cagacgtgct cgttcgccgc ctcgatatcc
     3121 tttttcgaga agcccaggtg tccgaggagg tcaaagccgg gttcgcttag cttctcggcc
     3181 tcgatctgca aggtgcgcgt gaggaactct tcgccgatcg tccaacgatt gaaggcgaac
     3241 ttgatatcga aggcggaccg caaggcggca tccagcttcg tcaagacttc gctgctaaag
     3301 ccgcgggctt tcaggctggc atggttgatg gccggcgcct gcgccaggga accgtggccg
     3361 accgcgtagg cctcgatctc ggcaatctgc gcctcgccgt agcccagcgt tcgcaacgcc
     3421 tccggcaccg cctggttgat gatcttgaaa tagccaccgc ccgccagctt cttgaatttg
     3481 acgagggcaa agtcgggctc gatgcccgtg gtatcgcagt ccatgaccag gccgatggtg
     3541 ccggtcggcg cgatcacggt cgcctgggca ttgcggtagc catgttgctg gccgagctcg
     3601 atggcccggt cccaggcgcg ttgcgcggcc tcggtcaggt gtgggtcggg acaggccacg
     3661 tgatcgagcg gcacgggggg gatggcaagc ttttcatagc cgtcccgttg cccgtaagcg
     3721 gcgcgccgat ggttgcgaac gacgcgcagc atgtcggccc tgttggcctc ataacccggg
     3781 aaggtgccga gttcgctggc catttccgcc gaggtggcgt aagcgacgcc ggtcatgatg
     3841 gcggcgagcg cgccacagat agcgcggccc tcggcgctgt cgtaggggat gcccgaggcc
     3901 atcaggcagc cgccgatgtt ggcgaagccg agcccgagcg tgcgatagcg ataggagagc
     3961 tcggcaatcc ggcgtgacgg gaactgcgcc atcatcaccg agatttccag ggcgatggtc
     4021 cacaggcggc aggcgtgctc gtaggcggcg acgtcgaagc gcttatcggg gccgcggaac
     4081 atcatcaagt tgagggaggc gagattgcag gcggtatcgt ccaggaacat gtactccgag
     4141 cacggattgg aggcgcggat cggtccggac ttggggcagg tgtgccaatc gttgatggtg
     4201 gtgtggaact gaatgcccgg atcggccgag gcccaggcgg cgtagccgat cttctcccac
     4261 agctcgcgcg ccttgacgct tttgacgggc ttgccgctca gccgcgccgt cagctgccag
     4321 tcgcggtcct ccagcatggc attgaggaac tcgtcggtga cgcgcacgga attgttggag
     4381 ttctggcccg agaccgtgag gtaggcctcg ctgtcccagt cggtgtcgta gctggcaaac
     4441 tcgaggtcct tatagccttg gcgggcaaac tggatgatgc ggcggatgta ggggtccggc
     4501 acgaggtcgc gccgcgcctc cttgatggcg cgcttgagcg cggggttttt ggcgggatcg
     4561 aagcaggcct cgccctccgc ctcgcaattg acgcaggcct tgagcagcgc cttcagccgc
     4621 ctctgacaaa gcttggagcc ggtgaccagg gcggccacct tctgctcctc cttcaccttc
     4681 cagtcgatat aggcctcgat gtcgggatga tcgatgtcga ccaccaccat cttggcggcg
     4741 cggcgcgtgg tgccgcccga cttgatggcg ccggcggcgc gatcgccgat tttgaggaag
     4801 ctcatcagcc ctgaggattt gccgccgccg gagagcttct cgttctcggc gcgcaggttg
     4861 gagaagttgg agccggtgcc cgagccgtac ttgaagaggc gcgcttcgcg cacccacaaa
     4921 tccatgatgc cgccttcgtt gacgaggtcg tcggcgatcg actggatgaa gcaggcatgc
     4981 ggctgggggt gctcgtaggc cgaggtcgag gccacgagct cgccggtttc gtgatcgacg
     5041 tagaagtggc cctggcccgg tccgtcgatg ccataggccc agtgcaggcc ggtgttgaac
     5101 cattgcgggc tgttgggggc gcccatttgc atggcgagca tgtagcggtg ttcgtcgaag
     5161 aaggcgcgcg catcgtcctc gttcgtgaaa tagccgccct tccagcccca gtaggtccag
     5221 gtgcccgcca ggcgatcgaa gacctggcgc gcgtcggtct ccccggtggt ccgctcatgg
     5281 cccgcacgct cgctgagcgc gcgctcatcg ggcaccgagc gccacagcca ggaggggacc
     5341 tgcgtctctt caatgcgctt caagcgcatg ggcacgccgg ccttgcggaa atacttttgc
     5401 gccaagatgt cggccgccac ctggctccag tgttcgggga ccgcgaaatt ctccagtttg
     5461 aagaccacgg agccgtccgg attcttgatc tccgaggtgg cgcgacggaa gccgattgtg
     5521 gcgtaaggcg atttgccctc cgtcgtgaag cgccgcttga tgtgcatgtc ttgcccccgt
     5581 ttggcatcgg cgctcgctgc ccgctcggcc tcagcggcgc cgtcatgttt gttgctcgag
     5641 cgtggaaaaa gggactggga ccggacgcca ccgacgcctc aacgcaacgg cacacacgac
     5701 caaccccacg cggcgtaaga tctcttgcgc ctcgcgtttt caacgcccgc ccttgacccg
     5761 acccttgggc ggacgcggcg gatgtggctg agggctccgc ggtcggctga ccggcacccg
     5821 taatgaccaa actatgacga ttcggaggct gtcgtcacga ggtcttgggg gtgtagataa
     5881 gaccagacac taaatgtagt aggtaggagg tttgaggggg cgatgctgtg gacagcgcgc
     5941 cccagaccgt cgctgcctca agggcttagg ccgcaagggc ccgcgccgcc gtccccgaag
     6001 ttgcgctttt cctgcaacgg gcatccccag gtcgtgcaca gataggccga ttctggcgcc
     6061 gctcagtgtg gcagatgtgc cgcggcaacg ggtgcatgga cagggccgca acggcgatgc
     6121 cataaggcgc ggcggacccc aaccccgggg ggaatgtgga gccgatgggc attttcagcg
     6181 aaatcttcag ttggtggggt ggcaacacct ggtcgaaccg cctctatacg gcgtggcgcg
     6241 gcagctatgt cggtagcgat gcggccggca accgctacta cgtgcagcgc cgcggcgtcg
     6301 gccccttggg cgtgccacgc cgctgggtca tctaccagca tggcgctgag gcctcccagg
     6361 tgccaccgga gtggcacggc tggatgcact acatggtgga caccccgccg agcgaggaaa
     6421 gctatacgcc gcacccctgg gagaagccgc accgcatgaa catgacgggg acggcggagg
     6481 cctaccgtcc ctcgggcagt attctgaaaa gcggccggcg gccaaaggca acgggcgatt
     6541 acaagccctg gcagccgaac tgaggcggcc cgccacgctg gcgccacctt ttgcgggctc
     6601 ctcggcggtg gcctcgctcg gcctttaacc ctcaaggcgc aagggcatgc gacgagacgg
     6661 tgtgcggcag gatttgcgac gggaaagaaa ggctgcggcc gcagcggcgc tgacggtcct
     6721 gctgctggct ctgcccgcgg cggcgcaacg catcgacaat gcggtcgcca tcttcgccgc
     6781 gctcgacaag gtcaccgcca agatttcccg gctcgaggtg cccctcaatc agaccgcgaa
     6841 gttcggtgcg ctcaagatca ccccgcgcgt gtgctattcg cgcgctccca ccgagccacc
     6901 aaagaccacc tccttcgtcg aggtcgacga aactcagctc gacggcaagg aaaagcgcat
     6961 cttctcgggc tggatgtttg ccgacagccc cgggctcaat gcggtggagc atcccgtttt
     7021 cgacgtttgg ctcaccgatt gcggccagcc gcaacgtgtc agcgggccgc ggccgccgcc
     7081 cggcgccgga ccggccgcca gcgagccgcc gccgccgagc gaacaggacc tgccgcgccg
     7141 ccggcgcgtg ccgcgctgaa gcaagctctg ccgaaattag cgcgcgttgc cgccggcaat
     7201 gatggcgagg atctcttcgg gcgccgtcgc cgtcggcagc gcggcgaagt cggcctggtg
     7261 cgccagggcc ttctccagca gggcatgaaa ggcgttgcgg tcgagctcca cggtgccgaa
     7321 ttggcgcaaa tgctcggtga cgaattgggt gtcgagcaaa ctgaagccgc cggcgatcag
     7381 gcgcgcgcac aaatagacga gcgctacctt gctggcatcg cgcgcgttgg agaacatgct
     7441 ctcgccaaag aaggcgccgt tgagcgccac gccgtaaagg ccgccgagca aacgggcgcc
     7501 ggcccaggcc tcgaccgtgt ggcagtagcc ggcgtcgaag aggtcgcggt agagcccgcg
     7561 aatgcgacgg ttaatccagg tcgtgcgccg tcccggccgc gacagggcgc agccgtcgat
     7621 cacggcctcg aagtcgtgat caatcttaat ttcgtagcta gcttgccgca cggtgcgggc
     7681 gaggcggcgg ggcacgtgca cggcgccgag cggtaggatg ccccgctgct gcggctcgat
     7741 ccagtagagc gcggggtcat cgcagctttc cgccatggga aagatgccgc aggcgtaggc
     7801 cttcaagagg acctgcgggg tgatttcgat gacgatgtcg tcgcgcgaag ccatcaatgg
     7861 gatcccggca ccgcccttgt cttagcgaat tatggttttt gcaggaagct ctcgagccag
     7921 tggatgtcgt aaagtccatt ggcgatatcg gaattttgca ccaattcagt aaacaacgga
     7981 atggtcgttt cgataccgtc gatgacgaac tcggacaggc aacgcttgag ccgcatcagg
     8041 cattcgttgc ggttcttgcc atgcacgatc agcttgccga tcaggctgtc gtagtagggc
     8101 ggaatgcggt agccctgata ggcgccgctg tcgacgcgca caccgaggcc tcccggcggg
     8161 tgccaatagg tgatggtacc cggcgacggc atgaaggttt tgggattttc cgcattgatg
     8221 cggcattcga tggcgtggcc cgagagggcg aactcgctct gcgaaatcga gagcggcgcc
     8281 ccggaggcga tgcggatctg ctcgatgacc aggtcgagcc cggtgatcat ctcggtcacg
     8341 gggtgctcga cctgcaggcg cgtgttcatt tcgatgaagt agaattcacc gtcctcgtac
     8401 aggaactcga ccgtgccggc accccggtac ttcagtttct gcatggcttt ggcgacgacc
     8461 tcgcccatgc cttgacgctg ctcggcgttg agcgccggcg agggagcctc ctccaggatc
     8521 ttctggtgcc ggcgttgcag cgaacagtcg cgctcgccca aatggatggc gttgcccttg
     8581 ccatcgccga acacctggaa ttcgatgtgg cgcggcttgg ccaggtactt ctcgagatag
     8641 acgctatcgt cgccgaaggc ggccttggcc tccgagcgcg ccgtcctgag ggcaagctcg
     8701 aagtcctcag gtccgcgcgc gaccttcatg ccgcggccgc cgccgcccgc ggcggctttc
     8761 accagcaccg gaaagccca
//
LOCUS       DASTMT010000110         4080 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_1042739, whole genome shotgun sequence.
ACCESSION   DASTMT010000110 DASTMT010000000
VERSION     DASTMT010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4080)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4080)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4080
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1042739"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..635)
                     /locus_tag="VFR00_02285"
     CDS             complement(<1..635)
                     /locus_tag="VFR00_02285"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU0158107.1"
                     /translation="MFVRAAAPRTVLITGASRGLGRALALAYAAPGRRLALLARNADL
                     LSYTARLCEMRGAEVLRRPCDVTDAAALAAFVAAIEAGGAIDLAIANAGEFWGNGAGG
                     ALEPLERARAQLRVNLEGAVLTVNAVLARMRERKAGHIALISSLAALTPLADAPAYSA
                     SKAGLAAYGIALRQMLAPEGIAVSIVYPGHIDTDQTDVHRGALPLLMLPDEA"
     gene            complement(639..1880)
                     /locus_tag="VFR00_02290"
     CDS             complement(639..1880)
                     /locus_tag="VFR00_02290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HEU0158108.1"
                     /translation="MLDKLPQARSARRLPFLPEPPPAPAPAEVAAPPAPQPDRRRPQR
                     QRRRILLWSLMVCVLLPTALASLYFGLIASDQFVVEVRFAVRSSNGGQSGDALSLFGA
                     IPNASSTVTDSYIVIDYLQSRELVDKLNERIGLRGIYARPEADYFSRFDDTLSVEEFV
                     KYWRKMLKAGFDVASQVVAVEVRAFSAEDAKKVATALLELSEELINEISARARADSVR
                     SSQQEVKRMEDLLRSHRGALRSFRDTQQEFDPTKQAEARYSLLGKLEEALSQAKTRLG
                     NLRSFMGKDAPSVLLLNSEIASLERQVAEERAKLGKDEATAARGRETLGGLVANYEEL
                     VVDREFAEKAYVSALSSLERARVEADRQQRYVAAFVRPTLPEEALYPRRIVASITVFA
                     IALVLWALGVLIVYAIRDHAL"
     gene            complement(1934..2620)
                     /locus_tag="VFR00_02295"
     CDS             complement(1934..2620)
                     /locus_tag="VFR00_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003533178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0158109.1"
                     /translation="MIQFENVRKAYRTKDEPKVILKNLSLTLPHANVGILGSNGAGKS
                     TLLRLIAGTEPPDAGVITRSGRISWPLGFSGSFNGSLSGAENCLFVARIYGQDTEYVL
                     DYVREFSELGSFFKMPVNSYSSGMRARLAFGVSMAIAFDYYLVDEITAVGDAQFQKKC
                     QQTFREKLSTSRIIMVSHQFATLRKYCDIGGVLRDGELTLYPTLTDAIRVHSGEPAED
                     ADADLEAIEG"
     gene            complement(2851..>4080)
                     /locus_tag="VFR00_02300"
     CDS             complement(2851..>4080)
                     /locus_tag="VFR00_02300"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEU0158110.1"
                     /translation="HELPFRRLALANDPPIVETVLIDRHGTFPKSAVVTVLLRPMIGA
                     GLFGQAGGEAVRSARRLFIQTLGRIGDEEVSRIANGLTAEYLARWRGAGDKLMVPREL
                     SRLTIDIVSQAMLGHCFSAEESVRFVDLFFAYHQRAAPLLLLLAPRNPRALEKLVRQL
                     GLEEIGTDMRALIATRFLAPIAAGEPAAGRAPFAKGLLEAHNGARESMLDEIAVMLLA
                     GHETTAATLSWLLWELAGDPANQDAVAKLLRGGAASGPQHQLWQDVAPAALRDALINE
                     TLRLYPPIAFFLRETTDALTLRDKPLRAGDFIVAAPWTLHRHLSRWRAADRFCPQRWL
                     AAPDRPAATAFLPFGFGARSCPGQRFAYLEMHAIVHQLLTGCRFTRLPVAAPKPLGSL
                     TSRPDRDFVLRLEPRQP"
BASE COUNT          718 a         1335 c         1400 g          627 t
ORIGIN      
        1 gcttcgtccg gcagcatgag aagcggcaag gcaccccgat gcacgtcggt ctgatcggta
       61 tcgatgtggc ctggatagac gatcgagacg gcgatgccct ccggtgccag catttggcgc
      121 agcgcaatgc cgtaagcggc aaggcccgcc ttcgacgcgc tgtaagcggg cgcgtcggca
      181 aggggcgtga gggccgcaag tgaggaaatg agggcaatat gccccgcctt gcgttcgcgc
      241 atgcgggcga gcacggcatt gacggtcaga accgcgcctt ccaggttaac ccgcagctgg
      301 gcgcgggcgc gctcgagggg ctcgagcgcg ccgccggcgc cgttgcccca gaactctccc
      361 gcgttggcga tcgccagatc gatcgccccg ccggcttcga ttgccgccac gaaggccgcg
      421 agcgccgcgg catcggtgac gtcgcacggc cggcgcagca cctcggcgcc gcgcatttcg
      481 caaagccttg cggtgtagct cagcaaatcc gcgttgcggg cgagcagggc caggcgccgt
      541 cccggggcgg cataggcaag cgccagcgcc cggccgaggc cgcgacttgc tccggtgatc
      601 aggacggtcc tcggcgcggc cgctcggaca aacatcgctc agagcgcgtg atcgcggatg
      661 gcgtagacga tcagcacgcc aagcgcccac agcaccaagg cgatggcaaa gaccgtgatg
      721 ctggccacga tgcgtcgcgg atagagggcc tcttccggca gcgtcggacg cacgaaggcg
      781 gccacgtagc gttgctggcg atcggcctcg acgcgcgccc gctcgagcga ggagagcgcg
      841 gaaacgtaag ccttctcggc aaactcgcga tcgaccacca attcctcgta attggcgacg
      901 agcccgccca aggtttcgcg gccgcgcgcg gcggtggcct cgtccttgcc gagcttggcc
      961 cgctcctcgg cgacctggcg ttcgagcgag gcgatttcgc tgttgagcag caggacactc
     1021 ggggcatcct tgcccatgaa ggagcgcaag ttgccgagcc tggtctttgc ctgcgacagg
     1081 gcttcctcga gcttgcccag cagggaatag cgcgcttcgg cctgcttggt gggatcgaac
     1141 tcctgttgcg tgtcgcggaa ggagcgcagc gcgccgcgat gcgagcgcaa tagatcttcc
     1201 atgcgcttga cttcctgctg cgagctgcgg acggagtcgg cgcgcgcgcg cgcggagatc
     1261 tcgttgatca gttcctcact cagctccaac agcgcggtcg ccactttctt ggcatcttcg
     1321 gcggaaaagg ccctgacctc gacggccacc acctgggatg caacgtcgaa gcccgctttc
     1381 agcatcttgc gccaatactt gacgaattcc tcgaccgaga gcgtgtcgtc gaagcgcgag
     1441 aagtagtccg cttccgggcg cgcgtagatg ccgcgcaggc caatgcgctc gttgagcttg
     1501 tccaccagtt cgcggctctg cagatagtcg atgacaatgt aggaatccgt caccgtcgag
     1561 ctggcgttgg gaatggcgcc gaacaggctg agcgcgtcac cggattggcc gccgttggaa
     1621 ctgcgcacgg caaagcgaac ctcgaccacg aactggtcgg aggcaatcag accgaaatag
     1681 aggctggcca gcgccgtcgg caacagcacg cagaccatga gcgaccataa gagaatgcga
     1741 cgccgctggc gctgcggccg gcgccggtct ggctgcggcg ccggcggcgc ggcgacctcg
     1801 gcaggggcgg gggccggcgg cggctcgggc aggaatggca gcctgcgtgc gctcctcgcc
     1861 tgcggtagct tgtccagcat gacacccgac ccccgtccaa cgctttgcgc cgccacagcc
     1921 aagcgcgccg gcgctatccc tcgatggcct ctaagtcagc gtccgcatcc tctgccggct
     1981 cccccgaatg gacgcggatc gcgtcggtca gtgtgggata gagggtgagc tccccgtcgc
     2041 gcaaaacgcc gccaatatca cagtatttgc gtaatgtggc aaactggtgc gagaccatga
     2101 tgatgcgcga agtagagagc ttctcgcgga aggtctgttg gcatttcttt tgaaactgcg
     2161 catcgcccac ggccgtgatc tcatcgacca ggtaatagtc aaaggcgatc gccatgctga
     2221 caccgaacgc cagccgcgcg cgcatgcccg acgagtagga gttcaccggc attttgaaga
     2281 acgagccaag ctcggaaaac tcgcgcacgt aatcgaggac gtactcggtg tcctgaccgt
     2341 agatgcgggc cacgaacagg cagttttccg cgcccgagag cgagccgttg aagctgcccg
     2401 agaacccgag cggccaggaa atgcgcccgc tgcgggtaat cacgcccgcg tcggggggct
     2461 ccgtaccggc gatcaggcgc aagagcgtcg acttacccgc gccattgctg cccaagatgc
     2521 cgacgttggc gtgcggcagc gtcaagctga ggtttttgag gatgaccttg ggctcatcct
     2581 tggtgcggta ggccttgcgc acgttctcga actggatcat tcggcatccc cgcgccatgc
     2641 cctgcgatcg ccgcgcgcct ggctcagcgc gcgagaagcc ggcggtcgcg gcaacgttgg
     2701 caccgtcaca ttgggctccc tcaggcgtgc gcgcgtcccc aagcgcttga ctttacgcac
     2761 ctttgccgcc gccattagta cagcaacgca agccttcgag ctagccgggc cggcaagggc
     2821 gcgattgaga gcggcggccc ggcctcgggg ctagggctgg cggggctcca ggcgcaggac
     2881 gaagtcgcgg tccgggcggg aggtaaggct gccgagcggc ttcggcgccg ccaccggcaa
     2941 gcgcgtgaac cggcaacccg tcaaaagctg gtgcacaatc gcgtgcatct ccagataggc
     3001 gaagcgctgg cccggacaac tgcgcgcgcc aaagccaaag ggcaggaagg ccgtggcagc
     3061 cggtcgatcc ggggccgcta gccatcgctg cgggcagaag cggtcggcgg cgcgccagcg
     3121 gctcagatgg cgatgcagcg tccagggcgc cgcgacgatg aaatcgcccg cgcgcagcgg
     3181 cttgtcgcgc agggtgagag catcggtggt ctcacgcagg aaaaaggcaa tcggcgggta
     3241 aaggcgcagc gtctcgttga taagggcgtc ccgcagcgcc gccggcgcga cgtcctgcca
     3301 aagctggtgc tggggtccgc tcgccgcgcc cccgcgcaag agcttggcca ccgcgtcctg
     3361 gttggcgggg tcccccgcca gctcccagag cagccaactg agcgtcgccg ccgtcgtctc
     3421 gtggccggcc aacaacatga cggcgatttc atcgagcatg ctctcgcgcg cgccgttgtg
     3481 ggcttcgagc aagcccttgg cgaaaggggc gcggccggcg gcgggctcac cggcggcgat
     3541 cggcgccaga aaacgggtgg cgatgagcgc gcgcatgtcg gtgccgattt cctctagccc
     3601 cagctggcgc acgagctttt cgagcgcgcg cgggttgcgg ggagccagca gcagcaaaag
     3661 cggcgcggcg cgctggtgat aggcgaagaa cagatcgacg aagcgcaccg attcctcggc
     3721 gctgaagcag tgccccagca tggcttgcga gacgatatcg atggtgaggc gcgacagttc
     3781 tcgcggcacc atgagcttgt cgcccgcgcc gcgccagcgc gcgagatatt ccgccgtcag
     3841 tccgttggcg atgcgcgaca cctcctcgtc gccaatgcgg cccaaggttt ggatgaacag
     3901 gcggcgcgcc gagcgcaccg cctcgccgcc ggcctgcccg aacaggcccg caccgatcat
     3961 tgggcgcaac agcaccgtca ccacggcgct cttgggaaac gtgccgtggc ggtcgatgag
     4021 caccgtctcg acaatgggcg gatcgttggc caaggccaag cgccggaacg gcagctcgtg
//
LOCUS       DASTMT010000111         7324 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_4296687, whole genome shotgun sequence.
ACCESSION   DASTMT010000111 DASTMT010000000
VERSION     DASTMT010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 7324)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7324)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7324
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4296687"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3458)
                     /locus_tag="VFR00_02305"
     CDS             complement(<1..3458)
                     /locus_tag="VFR00_02305"
                     /inference="COORDINATES: protein motif:HMM:NF017021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HEU0158111.1"
                     /translation="MSNFFVGLAVFCITVIGSLFAIPYFVDWNSYRGVFEDEASRFLG
                     REVRVSGAVNLNLLPTPSFRLERVRIADGANALREPFFRADSLAFKLSIAPLFRGIIE
                     ANEIEFRRPVLRLAREDRGGWNWQSFGQVFANAAYLPANVAITSLRISDGVLAVHGPG
                     GAERLRLEAIEGELSAPAIEGPYRFRGSFGKGGAEREIRIATAKPDGEAGVRLRSSLR
                     LSETGAAYLIDARLSDMMGEPKLDGELTARLPIGGLIRTEAARPAAGGSAEEERDGGP
                     QAFDLKAVLRADAGGGTLSDLALSFEQGGRPQLIVGEASARWQDTFECDVDLSSRWLD
                     LDRIAGTAQGARPSESLIPLALRTRELLPAEGHSRLTLAVDQANLGGEPVSGVRLAVA
                     RQKDRLAIEQFRVGMPGGSRAELSGTLAGSVAAPEFAGSLALRGASVLRALAWAAKPA
                     AALDSKGDGAFGLSTQLALSNGRLVARTIVGELMGTPLSGEAEWRWQGRPELSVRLEA
                     PQIDVRAFGPAEASLTDALHGLLYGAATEATGTANPATGRAEGPQADLRLSLEAGRLI
                     TATRTYRDVALTARLKGGNLEVPLLRLADEGGVALDLKGAIENIANRPKGSLSGVVAL
                     ASAADFGDMARLLGVPEALRPDQRLAEKLAPARLAGSLAFGARTATSRDLTLAGDVNG
                     ATLKMTGRLDGASTGWRAGPAEIVATLDAPDARKLVALLSEAAPGRADKAAPGQAFFK
                     AKGVPAEGLTSVLSLAAGDLSLDFEGRVSVLEQGHKIAGELRLKAADGERLAALAGLS
                     PPLPLAGVPVEGGLTLGLADGAVTLEHLLLKAGGRDVRGRILLRGAERRELDARLELS
                     ELSVRSLLAPVLDLRLAALTGAAEAALAGRQSAWPEEPFDLGALAGLDGHVVLALGRL
                     TLSDGLGLKDTTIDLNLKGGRIEVGSIEGAALGGRARIGFSLETSGSGAAVKGTLTLD
                     GARLEAATGLSAGAKAGTVGSLKGELAFSGRGANARNLVSALQGQGRLTLEGKLAGLW
                     PGAIALATEQVLKSEPERLRGVLKEALGQSLSLGRLNLPEDLVLELSEGQLRTRPILI
                     ESGADRAAGSATLNLLTLLFESDWRLQQVASKEATPANKPALPPLVVSFRGPLALLDN
                     VEPVVN"
     gene            3751..6120
                     /locus_tag="VFR00_02310"
     CDS             3751..6120
                     /locus_tag="VFR00_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015596489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEU0158112.1"
                     /translation="MATNRKSAPNSPAGPAPVGPAGSVSARALRPPSPPYMADLNPEQ
                     RTAVEALDGPLLVLAGAGTGKTRVLTTRIAHILCTGRARGSQILAVTFTNKAAHEMKD
                     RVGGLIGGAVEAMPWLGTFHAIGVKILRRHAELVGLKSGFTILDTDDQVRLMKQVIEG
                     AGLDKDRWPARQLAGLIDSWKNRGLTPAKVPRGEAFAFAEGKGAELYAAYQQRLKELN
                     AADFGDLLLEVLRLFLDNGDVLEDYRQRFRYILVDEYQDTNVAQYLWLRLLAGGGAPN
                     ICCVGDDDQSIYGWRGAEVDNILRFEHDFPGAKVVRLERNYRSKGHILAAAAHLIAHN
                     KARLGKTLFTDADMGRRVDLSGVWDDEEEARAIGQEIEAQQKAGRRLNEMAVLVRASF
                     QMRAFEDRFVTLGLPYRVIGGPRFYERQEIRDAIAYLEVTLNPDNDLKFERIVNVPKR
                     GLGDSTLKRISELARLRVRSLYQAAREIVDTEELAGKARKSLADLLASFERWRGQVAG
                     MKHTELAELILDESGYTQMWQQDRSPQAQARLENLKELVRFMHDFDTLEGFLEHVSLV
                     MDTDRADAGERVSLMTLHAAKGLEFELVFLPGWEEGLFPHQRSLDESGNAGLEEERRL
                     AYVGMTRAKQRLKVSFAQNRRNRGLWQAAAPSRFVDELPEAHLEIAEAKSPYGGGAAN
                     RFNPYGRSRFDEPAAGFASAYTTPGWQRAKEQWNREDRSKKRTPLTLEGELVASSAAA
                     AAGYALGERIFHQKFGYGRVAEVDGNKLTIDFDKAGRKRVVDSFVQRAP"
     gene            6303..>7324
                     /locus_tag="VFR00_02315"
     CDS             6303..>7324
                     /locus_tag="VFR00_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015596486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM143 family protein"
                     /protein_id="HEU0158113.1"
                     /translation="MASTGTMVRGGVWSVLANAAAAVRPAAEPAGKPPVREKFLPVTR
                     HALLDRLTHPNSWPAGEAREARRLMRYLDYWRRHSYAASLFELEQLYEPFSPDSDLLT
                     TRSFSADEMLAMQKQVVEQMRHFLVQANFEHVDPSDVKIILTKDSYYGLDLQVDLGAF
                     EEILIFYRGATTMSERRRTAKRLFLWHEEIKIPVFQRLFLLFKLKPFDVRVSEVMREQ
                     GLERKEAEKIVKRTRGRLPGSVKSDFIYMKLFKNIPRTDIEMIFPNTKVQFRLFDKIK
                     FGVTAGSGLGMGVAGTVGKIALVSTNPMALIGALAGLGGVAMRQASNFINQRNRYMIV
                     MAQNLY"
BASE COUNT         1210 a         2488 c         2464 g         1162 t
ORIGIN      
        1 ctgttgacga ccggctcgac attgtcgagc agcgccaagg gaccgcgaaa gctgacgacg
       61 aggggcggca gggccggctt gtttgcgggc gttgcctcct tgcttgccac ttgctgcagg
      121 cgccagtcgg actcgaatag caaggtcaac aaattcaagg tcgcgctgcc ggcggcgcgg
      181 tcggcaccgc tctcgatcag gatcggtcgc gtgcgcagct gaccctcgct gagttccaac
      241 accaggtcct cgggcaagtt gagccgcccc agcgacaggc tttggcccag ggcctccttg
      301 agcacgccgc gcagccgctc gggctcgctt ttcagcacct gttcggtggc aagcgcgatg
      361 gcgcccggcc ataggccggc aagcttgccc tcgagcgtga ggcgtccttg cccctgcagg
      421 gccgagacga gattgcgtgc gttggccccg cgcccgctga aggccaactc gcccttgaga
      481 ctgcccaccg ttcccgcctt ggcgccggcg ctgaggcccg ttgcagcctc gagccgggcg
      541 ccgtcgagcg tcagggtgcc cttgacggca gctccgctgc cactcgtctc cagactgaag
      601 ccgatgcgcg cgcgaccacc gagggcggcc ccttcgatgc tgcccacctc gattctgccc
      661 cccttcaggt tgagatcgat ggtggtgtcc ttcagcccca ggccatcgct gagggtcagg
      721 cggccaaggg cgagcaccac atgcccatcg aggcccgcca gggcgccgag gtcgaagggc
      781 tcctcgggcc aggcgctctg gcgacccgcg agcgccgcct ccgcggcacc ggtgagggcg
      841 gcaagtctca ggtcgagcac gggggcgagc aaggacctga cgctgagctc gctgagctcg
      901 aggcgggcat cgagctcgcg ccgctcggcg ccgcgcagga ggatgcgccc acgcacgtcg
      961 cgcccgccgg ctttgagcag aagatgctcc agcgtcacgg cgccgtcggc gaggccaagc
     1021 gtcagcccgc cctcgacagg aacgcctgcc agcggcagcg gcggggatag gccagcgagc
     1081 gcggccagcc gctcaccatc ggccgccttg aggcgaagtt cgccggcgat cttgtggccc
     1141 tgttccagca cgctgacgcg gccctcgaaa tcgaggctca gatcgccggc cgcgagcgac
     1201 agcaccgagg tgagcccctc cgcgggcacg cccttggcct tgaagaaggc ctggcccggc
     1261 gccgccttat cggcgcgccc aggcgccgcc tccgacagga gcgccacgag cttgcgcgca
     1321 tcgggggcat ccagcgtggc cacgatttcg gcggggccgg cgcgccagcc cgtgcttgcg
     1381 ccgtcaagcc ggcctgtcat tttcagggtc gcgccgttga catcgccggc gagcgtcagg
     1441 tcgcgcgagg tggcggtccg cgcgccaaag gcgaggctgc cggcgagccg cgcaggggca
     1501 agcttttcgg caaggcgctg atcggggcgc aacgcctcgg gaacgccgag gaggcgggcc
     1561 atatcgccaa aatcggctgc gcttgcgagc gccaccacgc cggacaggct gcccttgggc
     1621 cggttggcaa tgttttcgat ggcgcccttg agatcgaggg caacaccgcc ctcgtcggcc
     1681 aggcgcagca gcggcacctc gaggttaccc cccttgagcc gcgccgtaag cgccacgtcg
     1741 cgataggtgc gcgttgccgt gatgagccgc cccgcctcga ggctgagacg caggtccgcc
     1801 tgaggaccct cggcgcggcc cgtagcgggg ttcgccgtcc ccgtggcctc ggtggcggca
     1861 ccatacagca gcccatgcag ggcatcggta aggctcgcct cggccggacc aaaggccctg
     1921 acgtcgatct gcggcgcttc caggcgcacc gacagttccg gccggccttg ccagcgccac
     1981 tccgcctccc cggaaagcgg cgttcccatc agctcgccga cgatcgtgcg ggcgaccagg
     2041 cggccgttgc tgagcgccag ctgggtgctc agcccgaagg caccatcgcc tttgctgtcg
     2101 agggcggcgg ccggcttggc ggcccatgcg agcgccctca gcacgcttgc cccgcgcagc
     2161 gccaggctgc ccgcgaactc gggcgccgcg accgagccgg ccaacgtgcc gctgagctcc
     2221 gcgcggctgc cgccgggcat gccgacccgg aattgctcga ttgcgaggcg gtccttctgg
     2281 cgcgccacgg caagacggac gccgctgacg ggctcaccgc ccaaattcgc ctgatccacg
     2341 gccagcgtca ggcgtgagtg tccctcggcc ggcagcagct cccgggtccg cagcgctagc
     2401 gggatgaggc tttcgctcgg ccgcgcgcct tgcgcggttc cggcgatgcg gtcgagatcg
     2461 agccagcgcg aggacaggtc cacgtcgcac tcgaaagtgt cctgccagcg cgccgaggcc
     2521 tcgccgacga tgagctgcgg tctgccgccc tgctcgaagg agagcgccag gtccgagagc
     2581 gtgccgccgc ccgcatcggc ccgcagcacg gccttgaggt cgaaggcttg gggcccgccg
     2641 tcgcgctcct cttccgcact gccgccggcg gcgggccgcg cggcctccgt gcggatcagg
     2701 ccgccgatcg gcagccgggc cgtcagttcg ccgtcgagct tcggctcccc catcatgtcg
     2761 ctcagccggg cgtcgatcag gtaggcggca cccgtctcgc tgagccgcag ggagcttcgc
     2821 agccgcaccc cggcttcgcc gtcgggcttg gccgtggcaa ttcggatctc gcgctccgca
     2881 ccgcccttgc cgaagctgcc acgaaagcga tagggcccct cgatggccgg cgcagagagt
     2941 tccccctcaa ttgcctcaag ccgcaggcgc tcggccccgc cggggccgtg cacggccagc
     3001 acgccatcgc tgatgcgcag cgaggtaatg gcgacgttgg cgggcagata ggccgcgttg
     3061 gcgaacacct gcccgaagct ttgccaattc cagccaccgc ggtcctcccg cgccaggcgg
     3121 agcaccggcc ggcgaaactc gatctcgttg gcctcgatga tgccgcgaaa gagcggcgcg
     3181 atcgacaact tgaaggccag gctgtcggcg cggaagaagg gctcacgcag ggcgttggcg
     3241 ccatcggcga tccgcacgcg ttccaagcgg aaggacgggg tgggcagcaa attgaggttg
     3301 acggcgccgc tgacgcgaac ttcacggccc aaaaagcgcg aggcctcgtc ctcgaacacg
     3361 ccgcggtagg agttccagtc cacgaaatag gggatcgcga acaaggagcc gatcacggtg
     3421 atacagaaga ccgcgagccc gacgaaaaag ttgctcatcg gccgcctccg gacccgaggc
     3481 tggcagccgt cacccgcgca ggactgccgc gccgccggcg gcggcaagcc tgatcgccca
     3541 aagtgcgcgt tctatcgccg cttcgcctac gcattgccga cggatttgcc cgctcgcatt
     3601 tgtcgctttt cttttgcctt caactataca cgggatggcc agcaattaag accctcgaat
     3661 ttgccgccat cacggccttg gcgcgccgca attgtgggca gcggcgctgc ttcctttgcc
     3721 cggctgtggc cagtgctata gtcaactgca atggcaacga atcgcaaatc ggcgcccaac
     3781 tctccggcgg ggccggcgcc cgtgggcccg gcaggctccg tctccgctcg ggccctgcgc
     3841 ccgccgtccc cgccctacat ggccgacctc aacccggagc agcgcacggc cgtggaggcg
     3901 ctcgatgggc cgttgctggt gctggcgggc gccggcaccg gcaagacgcg tgtgctgacc
     3961 acgcgcattg cccatatcct ctgcacgggg cgggcgcggg ggtcgcaaat cctggccgtg
     4021 acctttacca acaaggccgc gcacgagatg aaggaccgcg tcggcggcct gatcggcggc
     4081 gccgtggagg ccatgccctg gcttgggacc tttcacgcca tcggcgtcaa gatcctgcgc
     4141 cgtcacgccg agctcgttgg gctcaagtcc ggcttcacca tcctggatac ggacgatcag
     4201 gtccgtttga tgaagcaggt gatcgagggc gccgggctcg acaaggatcg ctggcccgcg
     4261 cggcagctgg cggggctgat cgatagctgg aagaaccgcg gcttgacgcc ggcgaaggtg
     4321 ccgcgcgggg aggccttcgc ttttgccgag ggcaagggcg cggagcttta tgccgcatac
     4381 cagcagcggc tgaaggagct caacgcggcc gatttcggcg atctcttgct cgaggtgctg
     4441 cgcctgttcc tggacaacgg cgacgtgctc gaggactacc ggcagcgctt tcgctatatc
     4501 ctggtcgacg aatatcagga caccaacgtg gcgcagtacc tttggctgcg gctgctggcc
     4561 gggggtgggg cgcccaacat ctgctgcgtc ggcgacgacg accagtcgat ttacggctgg
     4621 cgcggggccg aggtcgacaa catcttgcgc ttcgagcacg acttccccgg cgccaaggtt
     4681 gtgcggctcg agcgcaacta tcgctccaag ggccacatcc tggcggcggc cgcgcacctc
     4741 atcgcccaca acaaggcgcg gctcggcaag acgctgttca ccgacgccga catggggcgg
     4801 cgcgtcgacc tcagcggggt gtgggacgat gaggaagaag cgcgcgcgat cggccaggag
     4861 atcgaggcgc aacagaaggc ggggcggcgg ctcaacgaga tggccgtcct cgtgcgggcc
     4921 tcctttcaga tgcgggcctt cgaagatcgc ttcgtcacgc tgggcttgcc ctatcgcgtg
     4981 atcggcggcc cgcgcttcta cgagcgccag gagatcaggg acgcgatcgc ctatctcgag
     5041 gtgaccctca atcccgacaa cgacctcaaa ttcgagcgca ttgtcaacgt gcccaagcgc
     5101 ggcctcggcg acagcacgct gaagcgcatc agcgagctcg cgcgcctgcg cgtccgttcg
     5161 ctctaccagg ccgcgcgcga aatcgtcgac accgaggaac tcgccggcaa ggcgcgcaag
     5221 tcgctggccg atttgctggc aagcttcgag cgctggcgcg gccaggtggc gggcatgaag
     5281 cacaccgagc ttgccgagct cattttggac gagtcgggct acacgcaaat gtggcagcag
     5341 gatcgcagcc cgcaggcgca ggcgcggctt gagaacctga aagagctcgt gcgcttcatg
     5401 catgacttcg acacgcttga gggtttcctg gagcatgtct cgctcgtcat ggataccgat
     5461 cgcgccgatg ccggcgagcg cgtctccttg atgacgctgc acgcggccaa ggggctcgaa
     5521 ttcgagctcg tgtttttgcc cggttgggag gagggcttgt tcccgcatca gcgcagcctc
     5581 gatgagagcg gcaatgccgg tctcgaagag gagcggcggc tggcctacgt cggcatgacc
     5641 cgcgccaagc agcgcctgaa agtcagcttc gcccagaacc ggcgcaatcg cgggctctgg
     5701 caggcggccg ccccctcgcg ctttgtcgac gagctacccg aagcccacct cgagatcgcc
     5761 gaggccaaaa gcccctacgg cggcggcgcc gcgaaccgct tcaatcccta tggccgcagc
     5821 cgcttcgatg agcccgcggc gggctttgcc agcgcctata cgacaccggg ctggcagcgc
     5881 gccaaggagc aatggaatcg cgaggaccgc agcaaaaagc gcacgccctt gacgctcgag
     5941 ggcgagctcg tcgcctcctc cgcggccgcg gcggccggct atgccctggg ggaacgcatt
     6001 tttcatcaga agtttggcta cggccgggtt gccgaggtcg atggcaataa gctcaccatc
     6061 gatttcgaca aagccgggcg caagcgcgtg gtcgacagtt ttgtgcaacg cgccccctag
     6121 tttccccctg tgcattttgc gtcacaggga cgtcccaaac gcccagtagt ctcgacaatc
     6181 gccgttggcc tcttctacaa cgaacaggct atttgagacg tgcgagttga ggcccgcttg
     6241 agccggggtt tcgatatttc gggccgaagg gagtgagttt caacgccagg cggggtggac
     6301 acatggcgtc gacgggaacg atggtgcggg gaggggtctg gagcgtgctc gccaacgccg
     6361 cggcggcggt gcgcccggcc gcagagcccg ccggcaagcc gccggtgcga gaaaaatttc
     6421 tgcccgtcac ccgccacgcc ctgctcgacc gtcttaccca ccccaacagc tggcccgccg
     6481 gtgaggcccg agaagcgcgg cgcctcatgc gctatctcga ctattggcgg cggcattcct
     6541 atgccgcgag cctgttcgaa ttggagcagc tctacgagcc gttcagtccc gacagcgatc
     6601 tgctgacgac ccgcagcttc agcgcggacg aaatgctcgc catgcaaaag caggtggtcg
     6661 agcagatgcg gcactttctg gtgcaggcga acttcgagca cgtcgatccc tcggacgtga
     6721 aaatcatcct gaccaaggat tcctactacg gcttggacct gcaggtggac ttgggggcct
     6781 tcgaggagat tttgatcttc tatcgcggcg ccaccaccat gagcgagcgg cggcgcacgg
     6841 ccaaacggct gttcttgtgg cacgaagaga taaaaattcc ggtctttcaa cggctcttcc
     6901 tgctgttcaa gctgaagccg ttcgacgtgc gggtcagcga ggtcatgcgc gagcaaggcc
     6961 tcgagcgcaa ggaggcggaa aagatcgtca agcgcacgcg cggtcgcttg cccggctcgg
     7021 tgaagagcga cttcatctat atgaagctct tcaaaaacat tccgcgcacc gacatcgaaa
     7081 tgatcttccc caataccaag gtgcagttcc gcttgttcga caaaatcaag ttcggcgtga
     7141 cggcgggcag cgggctgggc atgggggttg ccggcacggt gggcaagatc gcgctggtct
     7201 cgaccaatcc gatggcgctg atcggtgcgc tggctgggct tggcggcgtc gccatgcggc
     7261 aggcctccaa tttcatcaac cagcgcaatc gctacatgat cgtgatggcg cagaacctct
     7321 attt
//
LOCUS       DASTMT010000112         2217 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_5280817, whole genome shotgun sequence.
ACCESSION   DASTMT010000112 DASTMT010000000
VERSION     DASTMT010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2217)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2217)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2217
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5280817"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            181..1509
                     /locus_tag="VFR00_02320"
     CDS             181..1509
                     /locus_tag="VFR00_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011995192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEU0158114.1"
                     /translation="MAPLDMKPNELEPYWVPFTANRAFKKRPRLFAGAKGMHYFKPDG
                     SKVIDATSGLFCVNAGHCRAPIVEAIQDMAARLDYAPAFQYGHPKVFELASRLALLAP
                     GDLDYAFFSNSGSEAVDTALKIALGYHKVRGEGARTRFIGRERGYHGIGFGGISVGGI
                     VANRKQFGNMLTGVDHLPSTYDRAHQAYSRGEPEWGAHLADELTRLINLHDASTIAAV
                     IVEPVSGSTGILAPPKGYLQKLRELTAKHGILLIFDEVITGFGRLGQCFAAERYGVVP
                     DMITFAKGVTNAAVPMAGVMASKAIYQAFMTGAEHMVELFHGYTYSGHPLAAAAGLAT
                     LDIYKDEGLFERARSLETPFADAMMSLRGQPNVADIRTVGLLCGIDLEPIAGKPGLRG
                     FEATEHMYHDLNIYVRVALDSLIVAPPLISSEADIAEIRDGIAKVLKAVA"
     gene            1684..>2217
                     /locus_tag="VFR00_02325"
     CDS             1684..>2217
                     /locus_tag="VFR00_02325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0158115.1"
                     /translation="MAQQLGIPGPQSPHGRFADAFVIFSVTVLSMALGAWFLLQLGLS
                     LWPGMAAALTVYTVLLLFHLLVRRSLIDPSASEPREEMQWIAGEPELGAPASEARTGP
                     SFAPFSSTAAPSERIASELDHPHALPDASAEIAFHYRPRDAGGPLGGGRVSPRVGGNP
                     NPAHGPEEGWQGAAAPSP"
BASE COUNT          378 a          766 c          696 g          377 t
ORIGIN      
        1 gcagcggcaa aaaaccgcaa aaaaacaagc cgccaatcct gcgcccaagc gccatcctgg
       61 cgccgcgcgc taggccgcat acctgcccgc cgggatgggg gcggcccggc ggcttggagc
      121 cggccctggt ccctgctaga acgctcgcag cgatcagccc tgaaaaccgc ggagactgcc
      181 atggccccgc tcgacatgaa gcccaatgag ctcgagccct attgggtccc cttcaccgcc
      241 aatcgcgcct tcaaaaaacg cccgcgcctg tttgccggcg ccaagggcat gcactacttc
      301 aagccggacg gcagcaaagt catcgatgcc acgtctgggc tgttttgcgt caatgccggc
      361 cattgccgcg cgccgatcgt ggaagccatc caggatatgg cggcgcgcct cgattatgcg
      421 ccggcctttc agtacggcca tcccaaggtg tttgaattgg cctcccggct ggcgctgctc
      481 gcgcccggcg acctcgacta cgcatttttc tccaactcgg gttcggaagc cgtcgatacg
      541 gcgctcaaga ttgccctggg ctaccacaag gtgagaggcg agggcgcccg cacgcgcttc
      601 attggccgcg agcgcggtta tcacggcatc ggttttggcg gcatctcggt tggcggcatc
      661 gtcgccaacc gcaagcagtt cggcaacatg ctgacggggg tcgaccatct cccctccacc
      721 tacgatcggg cacaccaggc ctatagccgc ggcgagcccg aatggggcgc ccacctggcc
      781 gacgaactga cgcgcctcat caatctgcac gacgcctcga ccatcgccgc ggtcatcgtg
      841 gagccggtct ccggatcgac gggcattctg gccccgccca agggctattt gcaaaaattg
      901 cgcgagctca ccgccaagca cggcattttg ctgattttcg acgaggtgat cacgggcttc
      961 ggacgcctcg ggcagtgctt cgcggcggaa cgctacggcg tcgtgccgga catgatcacg
     1021 tttgccaagg gggtgaccaa cgcggcggtg cccatggccg gcgtgatggc gtcgaaggca
     1081 atctaccagg cgttcatgac cggcgccgag catatggtgg agctgttcca cggctacacc
     1141 tattccggtc acccgctggc ggccgccgcc ggcctggcca cgctcgacat ctacaaggat
     1201 gaagggctgt tcgaacgggc aaggagcctc gagaccccgt ttgccgatgc catgatgagc
     1261 ctgcgcggcc agcctaacgt ggcggatatc cgcaccgtcg gtctcttgtg cggcatcgat
     1321 ctcgagccga tcgccggcaa gccgggactg cgcggcttcg aggcgaccga gcatatgtat
     1381 cacgacctta acatctatgt gcgcgtcgcc ctcgatagcc tgatcgtcgc gccgccgctg
     1441 atctcgagcg aagccgacat cgccgaaatc cgcgacggca tcgccaaggt cctgaaagcc
     1501 gtagcctagg cacctgcgcg gcccctgccg gcagcggcgg cggcgccagg tcttgagcgc
     1561 cctcgtcccg aaggccggcc tggggaaacc tggtgatagt ccggccaagc cggttgcgca
     1621 tgcagccgcg ccgcaggaat gcttagccag ctcgaatcaa aaaaaagcgg agcttggctg
     1681 cacatggcac agcaactcgg catccccggc ccgcagtccc cgcatgggcg cttcgccgac
     1741 gcctttgtga ttttttcggt caccgtgctg tcgatggcgc tgggcgcctg gttcttgctg
     1801 caactgggcc tatcgctgtg gccgggaatg gcggcggcct tgaccgttta tacggtgctc
     1861 ctcctgttcc accttttggt gcgccgatcg ctgatcgacc ccagcgccag cgagcctcga
     1921 gaggagatgc aatggatcgc gggcgagccc gagctcggtg cgccggcgag cgaggcgcgc
     1981 accggcccct ccttcgcccc attttcgagc acggcggcgc cttccgaacg catcgcctcc
     2041 gaactcgatc atccccacgc actgcccgat gcgagtgccg agatcgcctt ccactatcgc
     2101 ccgcgcgacg ccggcggacc gctcggggga ggtcgcgttt cgccgcgcgt gggtggcaat
     2161 ccgaacccgg cacacggccc ggaggagggc tggcagggtg ccgccgcgcc gtcacct
//
LOCUS       DASTMT010000113         6215 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_1371330, whole genome shotgun sequence.
ACCESSION   DASTMT010000113 DASTMT010000000
VERSION     DASTMT010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 6215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6215
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1371330"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            50..718
                     /locus_tag="VFR00_02330"
     CDS             50..718
                     /locus_tag="VFR00_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019996427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEU0158116.1"
                     /translation="MHSELIALAQVIVIDLVLAGDNAIVVGLAASRVAPELRAKVIFW
                     GIAGAVALRITFAALTMQILAIVGLTFAGGILLLWVCWKMYRELRGGPRPASAGAAAV
                     AHANGEAVARPSFRAALVQIIAADVSMSLDNVLAVAGAAKGHTWVLVVGLAIAVVLMA
                     VAANYIAKLLARFPWITWIGLLIILYVAIDMMWRGSLELACTYGPAAMCGQGLIAFVA
                     EFLR"
     gene            1261..2046
                     /locus_tag="VFR00_02335"
     CDS             1261..2046
                     /locus_tag="VFR00_02335"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEU0158117.1"
                     /translation="MTHLAVNKALAKSSDDLHGRLADFAHGAGELNRPEDVLNALHTV
                     STSGLPLNVLGAMRLPVKSLDWDAVELGKSVFLHHDVPDGWWREHKVLAKSHFTPAIF
                     LAKSSLASFTWTELTRWLDPIGADRWSVELALKYGMRDGLTYGVGGRWIMVFWSRKEL
                     SHLLTPPDRILLAAAASFAALRLEQLVGADIDRIGSLPKLTARELAALRMVSVGRQTG
                     EIAKELAIGEETVRSHLKKAQAKLDACNRAHAACEALRLDLIP"
     gene            complement(2141..3982)
                     /locus_tag="VFR00_02340"
     CDS             complement(2141..3982)
                     /locus_tag="VFR00_02340"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU0158118.1"
                     /translation="MKFFCRLGVAAALLCSSMPPAVAGEPRRVLLLHSFGPHFAPWSA
                     VSGRFREELIKRSPDAIDLYEASLETARLAQAPDEVPFVEYLRSLFSGRDLDLVVAMG
                     APAARFVQRYRTQVFAATPLLITGSDERFISTAALTWNDTAVATKIDLSKLIENIFQV
                     LPDTSNIAFAIGNSALERLWVDELRRASQAFAQRASFEWWNEISLEEMLKRAATRPQH
                     AVIFYAGVRADARGVPHEEDRVFAQLHAAANAPIFGYADLNFGHGIVGGPHVSTQEIG
                     RQAAAVASRILRGEAPASITTPPLRLEAPAYDWRELRRWNISESALPAGSKVHFREPT
                     AWERYRWPLAAVFIALAIQGSMITWLLIERHARRNAEVDARNRLAEVIHLNRSAEAGA
                     LSASFAHELSQPLEAIMLSAATAERLLAPGAPESARLRQLLADIREADGHAVDIVQHL
                     RKLVKRSSEVELQEFDLNKVIANAVRILAPESRKRNVALRVNGKQQAFTVRADPIHLQ
                     QVILNLASNGMDAMTNAPASDRTITIQTASIGAACVEVSVSDSGPGIPEHKLAEIFDT
                     FYTTKAHGTGLGLSIARTIVETYGGKIWAENRDQGGAVFRFTLPLLC"
     gene            complement(4336..4662)
                     /locus_tag="VFR00_02345"
     CDS             complement(4336..4662)
                     /locus_tag="VFR00_02345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0158119.1"
                     /translation="MSQAYYQLLVDRLSGQIADRRSRRLAWVGDRYRKLAQINRHIVE
                     VRERIKRQRVQILSLQHEDRSTRRATALLRQLEMTLHLMNGHRAVILQRVRVCRCAPR
                     SSAAGR"
     gene            5408..5608
                     /locus_tag="VFR00_02350"
     CDS             5408..5608
                     /locus_tag="VFR00_02350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0158120.1"
                     /translation="MGERGEAAGRYAAAGELVIERAFGGDELCAGRDRLVLHRLIKAM
                     DAVFLVGAELQTLGELKHSGPG"
BASE COUNT         1065 a         1891 c         2081 g         1178 t
ORIGIN      
        1 gccgttcgcc gcctcgcaca ggcccgcatc aggagaggcg atggagacga tgcattccga
       61 gttgattgcg cttgcgcaag tgatcgtgat cgacctggtg ctggcgggcg acaacgcgat
      121 cgtcgtgggg cttgccgcct cccgggtggc ccccgagctg cgggcgaagg tcattttttg
      181 ggggatcgcc ggtgccgtcg ccctgcgtat cacctttgcg gcattgacga tgcagatcct
      241 cgctatcgtc ggcctgacgt ttgccggcgg catcctgctg ctgtgggtgt gctggaaaat
      301 gtatcgcgag ctgagagggg ggccgaggcc cgcctctgcg ggtgccgccg ccgttgcgca
      361 cgccaacggc gaggccgttg caaggccaag ttttcgggcg gccctggtgc aaattatcgc
      421 cgccgatgtt tcgatgtcgc tcgacaacgt gcttgccgtg gcaggtgccg ccaaggggca
      481 tacctgggtg ctcgttgtcg gtcttgccat tgccgtcgtg ctgatggccg ttgccgccaa
      541 ctacatcgcc aagctgctgg cgcgctttcc ctggatcacc tggatcgggc tgctgatcat
      601 cctttacgtc gccatcgaca tgatgtggcg tggctccctt gagcttgcct gcacctatgg
      661 gccggccgcg atgtgcgggc aaggtttgat cgcattcgtc gcagagtttc tgcgctgagc
      721 accggtgccg ttgggcgcgc tcccgcccga tcggggtgcc gtcgtcagcg cctaggggtt
      781 attgcgcggc actcgcccca agtctgaagt caagtcgcaa gctagtctcg cgttaagtct
      841 ggcgttgcga gcggctacgt tggcgcgcgc cctgggcggc aggcgaagct ttcggcttgc
      901 gctccgtcac cagcccgccc aacagcaggg ccgcgctgga cagcgtgaag gtcgcgatca
      961 gaagcagaaa aaagccaaag gcgcccatct tgcaagctct cggggaattg ccgcacggtc
     1021 gagcaggtgg gggcaggcgc ggccaatgcg gatcaccaaa aagggtgaga ccggcgccaa
     1081 actggttgca aggccgccgc tgttcgccgc gagggtgagc ttgccgcgcc gaacaggaga
     1141 ataggtcagc taacggagta aattgccggt tggtgcggtt ggggtttttc cggatgttgc
     1201 ggcgtggcga tccggcgcag aacagcccgg gggactaccc catggctttc gaggcgaccc
     1261 atgacacatt tggcggtcaa caaggctctc gccaagtctt ctgacgacct gcacgggcga
     1321 ttggcagact tcgcacacgg cgcgggggaa ttgaaccgac cggaggatgt gctcaatgcc
     1381 ttgcacacgg tgtccacctc cggcctgccg ctgaacgtgc tgggtgcgat gcgtttgccg
     1441 gtcaaatcac tggactggga cgccgtcgag cttggtaaat cggtcttcct gcaccatgac
     1501 gtgccggatg gctggtggcg cgagcacaag gtgctcgcga aatcgcactt cacgccggcg
     1561 atttttctcg ccaagtcgag tttggcctcg tttacctgga ccgagcttac gcgctggctc
     1621 gatccgatcg gcgccgatcg ctggtcggtc gagctcgctc tcaagtacgg catgcgcgac
     1681 ggcttgacct atggcgtggg cgggcgctgg atcatggtat tttggtcgcg caaggagctg
     1741 tcgcatctcc tgacgccgcc ggatcgcatt ttgcttgccg cggcggcctc ctttgccgcg
     1801 ctccgccttg agcagctggt gggcgcggac atcgatcgga ttggatcctt gccaaaattg
     1861 accgcgcggg aactcgccgc cttgcgcatg gtatcggtgg gccggcagac cggcgaaatc
     1921 gccaaggagc ttgcgattgg cgaagagacg gtacgtagtc acctgaaaaa ggcccaagcc
     1981 aagctcgacg cctgcaatcg cgcgcacgcg gcatgtgaag cgttgcgttt ggacctgatc
     2041 ccctgacgcg aacctgaaac ctcgacaagt cgcggcgcgc gcgggcgggc actacccata
     2101 cccaatcggc ggcggttttg cgccaaccgg ttggcgtttg ctagcacagc aacggcaggg
     2161 tgaaacgaaa gaccgccccg ccttggtcgc gattttccgc ccagatcttg cccccatagg
     2221 tctcgacgat ggtgcgcgcg atcgacaacc cgagcccggt gccgtgggcc ttggtggtat
     2281 agaaggtgtc aaaaatctcg gcaagcttgt gctcgggaat gcccggtccc gaatcgctga
     2341 ccgacacctc gacgcaggcg gcgccaatcg aggcggtttg aatcgtgatg gtgcgatcgc
     2401 tcgccggggc attggtcatg gcgtccatgc cattgctggc caggttcagg atcacctgct
     2461 gcaggtggat cggatcggcg cgtacggtga aagcctgctg tttgccattg acgcgcaagg
     2521 caacgttgcg ctttctcgat tcgggggcga gaatgcgcac ggcattggcg atcactttgt
     2581 tgaggtcgaa ttcctgcaac tcgacctcgc tgctgcgctt taccagcttt cgcaagtgct
     2641 ggacgatgtc gacggcgtgc ccgtcggcct cgcgaatatc cgccagcagc tgccgcagcc
     2701 gggcgctctc gggtgcccct ggcgccagca ggcgctcggc cgtggcggcg ctgagcatga
     2761 tggcttccaa cggttggctc aactcgtgcg caaacgacgc cgacaacgcc ccggcttcgg
     2821 ccgatcggtt cagatggatg acctcggcga ggcggttgcg ggcgtcgacc tcggcattgc
     2881 gacgggcgtg acgttcgatc agcagccagg tgatcatgct gccttgaatg gcgagcgcga
     2941 tgaacaccgc ggccagcggc cagcggtagc gctcccacgc cgtcggctcg cggaaatgca
     3001 ccttgctgcc cgctggcagc gcgctctcgc tgatgttcca acgcctcagc tcacgccagt
     3061 cgtaggcggg cgcctcaagt cgcaacggcg gcgtcgtgat gctggccggg gcctcgccgc
     3121 gaagtatgcg gcttgcgacc gctgcggcct gtcgaccgat ctcttgcgtg gagacatgcg
     3181 gaccaccgac gatgccatgg ccgaaattga ggtcggcata cccgaagatc ggtgcgtttg
     3241 cggccgcgtg aagttgggca aagacacggt cctcctcgtg cggcacaccg cgcgcatcgg
     3301 cgcgaacgcc ggcataaaat atcacggcgt gctgcggtcg cgtcgccgcg cgctttagca
     3361 tttcttcgag tgagatctcg ttccaccact cgaagctggc gcgctgggcg aaggcttgcg
     3421 aggcgcggcg caattcatca acccagagcc gctccagcgc cgagttgccg atggcaaagg
     3481 caatattgct ggtgtccggc agtacctgga aaatattttc gatcaacttt gacaagtcta
     3541 tcttggtcgc gactgccgta tcattccacg tcagcgctgc cgtgctgata aagcgttcgt
     3601 cggatcccgt aatgagcaat ggcgtggcgg cgaagacctg cgtgcgatag cgctgcacaa
     3661 agcgggcggc gggcgcgccc atggcgacga cgagatcgag gtcacgcccg ctaaaaaggg
     3721 aacgcaggta ttcgacaaac gggacttcgt cgggagcctg cgccaggcgt gcggtctcga
     3781 gcgaggcctc gtagaggtca atggcgtcgg gcgaccgctt gataagctcc tcgcggaagc
     3841 ggcccgagac ggcgctccaa ggcgcaaagt gcggcccaaa ggagtgcagc aagagaacgc
     3901 gccgcggctc gccggctacc gccggcggca tgctcgagca aagcagggct gccgccaccc
     3961 ccaatcggca aaaaaatttc atccccagca attccccgcg gatgggccta acggcctcgg
     4021 tacggaagcg tacataccat ccgcgctcgg agggtggaaa tacgggaaaa cccctgaaaa
     4081 ttaaccgcta ttgcgggcga tcggtggttt tgcgggcgtg aagttacccc gccgggccgt
     4141 gcttggcccg ggggcctcgt tatgccgggt taacaatagg ctggggcccg tccgggaagc
     4201 cgcaagccgt tgacgccttg ctcggggaat agcgtttgaa acaaacggtt tcggcttcgg
     4261 cgcgcccgcg tcgcgccgat gaccacgctg gcgctggggc ggcttgcagc gaggcggggc
     4321 ccaggcgaag tgagctcagc ggccggccgc gctggaacgc ggcgcgcatc ggcagacgcg
     4381 cactctctgt aagatgacgg cgcgatgtcc gttcatcaga tgcaacgtca tctcgagctg
     4441 tcgcagcagt gcggtggccc ggcgggtgct gcggtcctca tgttgtaggc tgagaatttg
     4501 caccctttgg cgcttgatgc gctcgcgcac ctcaacgatg tggcggttga tttgtgcaag
     4561 ttttcgatat ctgtcaccga cccaggcgag gcgacggctc cgccggtcgg cgatctggcc
     4621 gctcagacga tcaacgagca gctggtagta agcttgtgac atgatccgca cttcaacccg
     4681 aacctaacag cgggcttgca agagatctac tgggacgatg ctcatggctg tcatgtcggc
     4741 gggctgccgg caatcgcgcc ggtgtgaaga cgatcatcat cgccggtgcc aacgaacggg
     4801 gcgcctggtt aacgggagtt ggaccattcc ctcgagctcg cgcatcagca tggcgcgata
     4861 ctggatcttt gcctgcaagg agcgctcgag cacacttaac aggccttcgg ggtcgccgcc
     4921 gtcgtgtcgg cttcttgcca cgacccgggc ccgctcaagg gcgacgcgat cccgtccctc
     4981 ggcaatgcgt cggttaaact ttgcaagctg acggtaggtg gcacgttttt gcatggggac
     5041 cccgcccggc aatttgaggc gtggtcctag catcgcgccc agccccgctc atcggaccaa
     5101 ggtcgctacc aattcgaccg accggctgca gcctaatctt tgccgtaact atgtgaaccg
     5161 agctttggcg ccaaaatccg cggcgctttc gcggcgaaaa agcccttctc ggaggcccga
     5221 tggcggcggc atcgccaaac cgtcggcgcg gccggctgct cttggttaag ggcaccgtca
     5281 acgtggcgag cgatagccgc gcagctcggc gtatcgccac ctttgctcgg gggtgagcac
     5341 cgccgccgtt gtgagatggt actgcaaatg aacggcgcgg aggctcgccc tgcggcgcgc
     5401 cgatcgcttg ggtgagcgag gcgaggctgc cgggcgttat gctgccgccg gcgaactggt
     5461 gatcgagcgc gcgttcggcg gcgatgagct ttgcgccggc cgcgatcgcc tcgttcttca
     5521 tcgcctcata aaggctatgg acgcggtgtt tctagtcggg gctgagctgc agaccctcgg
     5581 cgagctcaag cacagcggac ctgggtagcc gttgagttcc gcactaagcg ccaggcccat
     5641 gccgcgccca gcttgccaat cggcgagctg ctgctcgctc agggccttga tgtcgcgtgc
     5701 gtcgagaccg acgtaaggcg agtgttgatg gtgatgttgg gccagggcgg gcccggcgag
     5761 ggatagcaaa actgctgcca cgactaagcg cattcgattg attactgaga cgaggatgac
     5821 agaggcagct gcctgccgcc taccggaatg aacatcggcg tcctgcgcat ctcgggcgtt
     5881 ggcaaaaggg gcgcgcaaca tgcgtgcggg ctgttaggga tgttgtcatg catggaccgt
     5941 gccatcggtc tgtgatcgag atcacagccg gccgatcctc acgtgcttag catcgccaag
     6001 gctatcgggg cgtgggcgcg tgctatgtct ggcgtcgggg tgcgtgcaag acggtcgctt
     6061 ccccgcgggg aacacccgat agccacccga atcccgccgg tgccgggcgc aattgcccgc
     6121 gcgtcaggat tattgacccg cgaggcccaa gcaggcttgg gctttttgtg acgggaggcg
     6181 gtatccaccc gttagcgccc cgggcttggg cgcct
//
LOCUS       DASTMT010000114         3559 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_3998489, whole genome shotgun sequence.
ACCESSION   DASTMT010000114 DASTMT010000000
VERSION     DASTMT010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3559
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3998489"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            321..653
                     /locus_tag="VFR00_02355"
     CDS             321..653
                     /locus_tag="VFR00_02355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0158121.1"
                     /translation="MNGAPGDGGTALASALQRELSGKGITVGGKSASAYRVEGTVTMG
                     AAKEGKQPIQIEWLVRDPQGKKLGTVSQRNEIPEGSLDGPWGKTAEQAAGAAAQGILK
                     LLPQKAVN"
     gene            823..1755
                     /locus_tag="VFR00_02360"
     CDS             823..1755
                     /locus_tag="VFR00_02360"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HEU0158122.1"
                     /translation="MKLVAGNSNRPLAEAIAAALKLSLCRTVVRRFADMEVFVEVQEN
                     VRGQDVFILQSTSFPANDNLMELLILVDALKRASAQRITAVMPYFGYARQDRKPGPRT
                     PISAKLVANLIERAGVDRVMTVDLHAGQVQGFFDIPTDNLFAAPVMVRDIQERYGTSN
                     LVVVSPDVGGVVRARGLAKRINVPIAICDKRRERAGESEVMNVIGEVKGKRCILIDDI
                     VDSGGTLVNAANALLEEGATEVLAYVTHGVLSGGAVAKITGSRLKTFVITDTIQPTEA
                     VKAASNIRVLPMAPLLAEAIARTAREESVSSLFY"
     gene            complement(1749..3152)
                     /locus_tag="VFR00_02365"
     CDS             complement(1749..3152)
                     /locus_tag="VFR00_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011666243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEU0158123.1"
                     /translation="MTNAASTIASPPSPPQRGTDNLLLQAPLLPTLLGMALPNLAALL
                     VAAAVAIAETSYVGVLGTAPLAAIALVFPVIMLMQMLSSGAMGGGVSSAISRALGAGD
                     DARATALAMHALAIGAGAGLCFSLLFSLFGRQLFTALGGRGAVLEQALAYANVALSAA
                     VLLWLLNTLASIVRGTGNMQIPALTLLGVSALQIGCGGALGLGVGPIPRLGLPGVASG
                     LAIGSATGTGFLIWYLCSGRGRLRLRLAGVPFRREMFADILSVGAVAAFSPLQSVLTV
                     LILTRAIASFGTEALAGYGIGARLEFLLVPIAFAVGVACVPMVGMAVGAGRIARARQV
                     AWTGALVSALALGGIGSLVATLPHLWSGLFSAEASVTAAANLYLRCAGPAFPFFGLGL
                     CLYFASQGAGKVLGPVLASSLRLAIIAIGTVVLTWNHAPLWTLFVLVGGSLVAYGAAT
                     AVAVYATAWGSPKAGAQ"
     gene            3288..>3559
                     /locus_tag="VFR00_02370"
     CDS             3288..>3559
                     /locus_tag="VFR00_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008963752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU0158124.1"
                     /translation="MAQHYGQVCPLARALDVVGERWALLILRDLKRKGPLRFHAIEAG
                     LPGAAPNTLSARLKALEAHGVIATRLYERHPPRYEYFLTQKGQDLAP"
BASE COUNT          727 a         1159 c         1163 g          510 t
ORIGIN      
        1 tggcatcctc gggtggctcg aaggatccat gggcggcgct cacgcccacg gttgcgcagt
       61 cgatcgccgg taaggccgcc aattcctttg catcctggct cgccacgcgg ggccaatcgg
      121 ccgttgccag cgccaccgcg catcccgcgg gcgttggcgc ccaaagcact gcggaaggta
      181 cacaggcaga ggcgcgcgca tcggcgccgg ccaggcaagc cgtggcggcc gccccgcctg
      241 cggcgcaacc gcccgccgcg agcgcttcct cgacgggcag catcagcagt gacggccctc
      301 tggccgccat ggtgccgaac gtgaacggag cgcctggtga cggtggcact gcgctggcga
      361 gcgccctgca acgcgaattg tcaggtaagg gtatcaccgt tggcggcaaa tcggcatcgg
      421 cctatcgcgt agagggtacg gtgacgatgg gggcggccaa ggagggcaaa cagccgattc
      481 agatcgaatg gctggtgcgg gacccgcagg gcaagaagct cggcaccgta tcacaacgca
      541 acgagatacc cgaggggtcg ttggacggcc cgtggggcaa gacggcggag caggcggccg
      601 gggcggcggc gcagggaatt ctcaaacttt tgccgcagaa ggccgttaac taacgacaat
      661 gggctgaacg ctgcttagcg tcccgtgggc gggttccaac acccgcccac accgtttaca
      721 gggggccaat gtccttgcta gaaggccccg gcagtgccaa cggagcgcgc gttgatgccc
      781 ttcgataccc gagacgcgaa ccgtggccgg ggggcgctga agatgaaatt ggtggccggc
      841 aactccaatc gccccctcgc ggaggccatc gccgcagcct tgaagctctc gctctgccgc
      901 accgtcgtgc gccgcttcgc cgacatggaa gtcttcgtcg aggtgcaaga gaacgtgcgc
      961 gggcaggacg tcttcatcct gcagtcgacc tcgttccctg ctaacgacaa cctgatggaa
     1021 ttattgatcc tggtggacgc cctcaagcgc gcctccgcgc agcgcattac cgcggtgatg
     1081 ccctatttcg gctacgcccg ccaggaccgg aaaccaggac cacgcacgcc aatctcggca
     1141 aagctcgtcg ccaacctgat cgagcgggcc ggcgtggatc gcgtgatgac ggtcgatctg
     1201 cacgccggtc aggtgcaggg gttctttgat attccgacgg acaacctgtt tgccgcgccg
     1261 gtcatggtgc gcgatatcca ggagcgttac ggcacctcga acctggtcgt ggtgtcgccc
     1321 gacgtcggcg gcgtggtacg cgcgcggggc ctggccaagc gtatcaacgt tccgatcgcc
     1381 atttgcgaca agcgccgcga gcgagcgggc gagtcggagg tcatgaacgt cattggcgaa
     1441 gtcaaaggca agcgctgcat cctcatcgac gacatcgtcg attcgggggg aacgttggtc
     1501 aacgccgcca acgccctgct cgaggaggga gccacggaag tgttggccta tgtcacccat
     1561 ggggtgctgt cgggcggcgc cgtcgccaaa atcaccgggt ctcgcctcaa gacctttgtc
     1621 ataaccgaca ccatccagcc gaccgaggcg gtcaaggccg ccagcaatat tcgcgtgctt
     1681 cccatggccc cgctgctggc ggaggccatc gcacgcacgg cgcgcgaaga aagcgtctcc
     1741 agtctgttct actgagcgcc tgcctttggt gagccccagg ccgtcgcata gaccgcgacc
     1801 gccgttgctg ccccataggc aaccagcgat cccccgacca gtacgaacaa ggtccacagc
     1861 ggggcgtggt tccacgtcag cacgaccgtg ccaatggcaa tgatggcaag ccgcaggctc
     1921 gaggccaaca cgggaccaag caccttgccc gcaccctgcg aggcaaaata gaggcaaagt
     1981 cccagtccga agaaaggaaa ggccggaccg gcacagcgta agtagagatt tgcggcggcc
     2041 gtaacgctgg cctcggcgct gaacaagccg gaccacagat gcggcagcgt cgccaccagc
     2101 gaaccgatcc cccccagggc aagcgccgaa accagcgccc cggtccaagc cacctgacgc
     2161 gcgcgcgcga tgcgaccggc acccacggcc atgccgacca tgggcacgca agcaacgccg
     2221 accgcgaatg caatcggaac cagcaggaat tcgaggcgcg cgccgatgcc atagccggcc
     2281 aaggcctcgg tgccgaagct cgcaatcgcc cgggtcagga tcaacaccgt caagaccgac
     2341 tgcaggggcg agaatgcggc gacggcaccc acgctcagga tatcggcaaa catctcccgg
     2401 cgaaaaggca ctccggccaa ccgcagccgc aggcgtccgc gccctgagca caggtaccag
     2461 atgaggaagc ccgtccccgt cgcactgcca atggccaagc cggaggccac gccgggcagg
     2521 cccaagcgcg gaatgggacc gacgccgagg ccaagggcgc cgccgcaacc gatttgcagg
     2581 gccgatacgc caagcagcgt caacgcgggg atttgcatgt tgccggtgcc gcgcacgatg
     2641 gaagcgagcg tattgagcag ccaaagcaag accgccgccg acagggccac attggcatag
     2701 gccagggcct gctcgagtac ggccccccgc ccgcccaggg cggtaaacaa ttgccggccg
     2761 aacaagctaa acaggaggga aaagcacagc cccgcaccgg caccgatggc caacgcatgc
     2821 atcgccaaag ccgtagcgcg cgcatcgtcg ccggcaccca gggctcggct aatcgctgag
     2881 gaaacacctc cgcccatggc gcccgacgac agcatttgca tgagcatgat caccggaaag
     2941 accagagcga tggcggcgag cggcgccgtg ccgagcacgc cgacgtagga ggtttcggca
     3001 atcgccacgg cagcggcaac gagcagggcg gcgagatttg gtaacgccat gcccagcagg
     3061 gtcggcagca agggcgcttg cagcagcagg ttgtcggtgc cgcgctgggg aggggaggga
     3121 ggcgatgcga ttgttgaggc tgcgttggtc aacaaaacca ctccctgact gtagtcaggc
     3181 caagccaaac gcggtccggt ccggcggcgc cgtagttata gatagtaaag ttacttgtca
     3241 atctagagcg acttgcttgg caggaatgag aggagtacac tgcatgcatg gctcaacatt
     3301 atggccaggt ttgtccgctc gcgcgcgccc tcgacgtcgt tggcgaacgc tgggcgctat
     3361 tgatcctgcg cgatctcaaa cgcaaaggac ccttacggtt tcacgcgatc gaggcgggat
     3421 tgccgggcgc agcgcccaat acgttgtcgg cgcgtctcaa agccttggag gcgcacggcg
     3481 tgatcgcaac gcggctctac gagcgccacc cgccgcgcta tgaatacttc ctgacgcaaa
     3541 agggacagga tttggcgcc
//
LOCUS       DASTMT010000115         1932 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_3014101, whole genome shotgun sequence.
ACCESSION   DASTMT010000115 DASTMT010000000
VERSION     DASTMT010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 1932)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1932)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1932
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3014101"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..482)
                     /locus_tag="VFR00_02375"
     CDS             complement(<1..482)
                     /locus_tag="VFR00_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018319233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEU0158125.1"
                     /translation="MSERTWHAVLGALPPVCLIALWHGIASAGWAPAALLPLPSAVFA
                     RLFEQLHNPVFLQHAATTLYRLFAGFGIAVVVGVALGVAAASNRSFEALLRPLLRVLA
                     PVPKIALYPALVLLLGFEHASKVALVVADAVFPILLATYQGAASVEAKLVWSARAAGA
                     S"
     gene            complement(482..1267)
                     /locus_tag="VFR00_02380"
     CDS             complement(482..1267)
                     /locus_tag="VFR00_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006614888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0158126.1"
                     /translation="MNRGVSLAAGLPPLLACLGLLALWEAAARSFDVNGLPPASAALR
                     ELPAILGDREALLNIAASLRRMLTGFVLALLIALPLGLAMARSPRVAALCNPLLMIIY
                     PVPKAALMPIIMLWLGIGDASKTIVIGLGVSLPVIYHSYQGARAVEEKLIWSAAAMGM
                     RPPARLLRIVLPAALPQIFVGIRTGLVLALITMVTSEMIARQAGVGSILFNALDMAQY
                     ANVYAIILIIGALGFVLDLLFERLRARLVAWAEPSHDIAVGTA"
     gene            complement(1264..>1932)
                     /locus_tag="VFR00_02385"
     CDS             complement(1264..>1932)
                     /locus_tag="VFR00_02385"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020607888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0158127.1"
                     /translation="VAILGPSGCGKSTLLYVVGGFVPPSSGKVLVNGRAVTGPGPDRG
                     PIFQEFALFPWKTVLGNVMYGLREQGVPKAQAESKARTLISLVHLAGYEDFYPKQLSG
                     GMKQRVAIARTLAYGPKILLMDEPFGALDAHTRTGLQRELLEIWERDRKTVLFVTHSV
                     EEAVFLADRVVVLTRSPGRIKAMIDVDLPRPRRRAELLVDRRYQTLVVEIEGLMDSQS
                     QGQQ"
BASE COUNT          387 a          625 c          643 g          277 t
ORIGIN      
        1 gaggcccccg ccgctcgggc cgaccacacc agcttggctt cgacgctggc ggcaccctgg
       61 taagtggcga gcaggatcgg gaagacggca tcggcaacga ccagggccac cttcgaggca
      121 tgctcgaaac cgagcagcag caccagcgcg gggtagagtg cgatttttgg caccggcgcc
      181 aaaacgcgca gcagcggccg cagcagcgcc tcgaagctgc ggttgctggc ggcggcaacg
      241 cccagcgcca cgccgaccac gacggcgatc ccgaacccgg caaatagccg gtacagcgtg
      301 gtggcggcgt gctgcagaaa cacgggattg tgcagctgct cgaacaggcg cgcgaatacg
      361 gccgacggca atggcaacag tgccgcgggt gcccatccgg cgctggcgat gccatgccac
      421 aaggcgatca ggcagacggg cggcagcgcc cccagtacag cgtgccatgt gcgctcgctc
      481 atcacgccgt ccccacggcg atgtcatggc tgggttcggc ccaagctacc agcctggcgc
      541 gtagccgctc aaacagcagg tcgagcacaa accccagcgc cccgatgatg agaatgatgg
      601 cgtagacatt ggcgtattgc gccatatcga gggcattgaa gagaatgctg ccgacgcccg
      661 cctggcgcgc gatcatctcg ctggtgacca tggtgatcag ggccagcacc aagccggtgc
      721 gaatgccgac aaagatttgc ggcaatgccg ccggcagcac gatgcgcagc aggcgcgcgg
      781 gcggcctcat gcccatggcc gcggccgacc aaatcaactt ctcctcgacg gcgcgcgcgc
      841 cctgatagct gtggtagatc accggcaagc tgacgcccag gccgatgaca atggtcttgg
      901 aggcatcgcc gatgccaagc cacaacatga tgatcggcat caaggccgcc ttcggaacgg
      961 gataaatgat cattagcagc ggattgcaga gggcggcgac ccttgggctg cgcgccatcg
     1021 ccagcccgag ggggagcgca atgagcaacg ccagcacgaa gccggtcagc atacgccgca
     1081 gcgaggcggc gatattgagg agcgcctcgc ggtcgccgag gatggcgggc agttcgcgca
     1141 gggcagcgga ggccggtggc aggccattga catcgaaact gcgcgcggca gcttcccaca
     1201 gcgccagcag gccgaggcaa gcgagcagcg gcggcaggcc cgcggccaat gaaaccccgc
     1261 ggttcattgc tgtccttggc tctggctatc catcaagccc tcgatttcca cgaccaacgt
     1321 ctggtaacgc cgatcgacca gcaattcggc gcgtcggcgc gggcgcggca gatcgacgtc
     1381 gatcatggct ttgatgcgac cgggcgagcg ggtgagcacg accacacgat cggccaaaaa
     1441 taccgcttcc tccacgctgt gggtgacgaa cagcacggtc ttgcgatcgc gttcccaaat
     1501 ctcgagcaac tcgcgctgca gacccgtgcg ggtgtgcgca tcgagcgcgc cgaagggttc
     1561 atccatcaac aggattttcg ggccataggc cagcgtgcgt gcaatcgcga cacgctgctt
     1621 catgccgccc gaaagctgct tggggtaaaa atcctcatag ccggcgaggt gcaccaggga
     1681 aatcagggtg cgggctttgc tctcggcctg cgccttcggc acgccctgct cgcgcaggcc
     1741 gtacatgacg ttgccgagca cggttttcca gggaaacagg gcgaactctt ggaagatggg
     1801 gccgcgatcg ggtccgggtc ccgtcaccgc cctgccgttg acgaggactt tgccgctgct
     1861 cggcggcacg aagccgccga ccacgtagag caaggtcgac ttgccgcagc ccgaagggcc
     1921 gagaatggcg ac
//
LOCUS       DASTMT010000116         1995 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_3342529, whole genome shotgun sequence.
ACCESSION   DASTMT010000116 DASTMT010000000
VERSION     DASTMT010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 1995)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1995)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1995
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3342529"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..671
                     /locus_tag="VFR00_02390"
     CDS             <1..671
                     /locus_tag="VFR00_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosylglycinamide synthetase C
                     domain-containing protein"
                     /protein_id="HEU0158128.1"
                     /translation="LATAQDHKRAFDGDQGPNTGGMGAYSPAAVMTPQLISATMDNII
                     RPTVRAMAERGTPFKGVLFAGLMLTEAGPKLIEYNVRFGDPECQVLMPRLKSDLLAAL
                     LATVDGVLGSFDLRWHEDAALTVVLAARGYPGAYAVGTEIRGLEAAAALENVEVFHAG
                     TAREGARLTAVGGRVLGVTARAATVSEARRRAYAAVARIDWPEGFYRTDIGWRAMERE
                     QRGA"
     gene            772..1761
                     /locus_tag="VFR00_02395"
     CDS             772..1761
                     /locus_tag="VFR00_02395"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEU0158129.1"
                     /translation="MSEATRTNALTARKGGPLVLRPSIALALGGGGARGLAHILMLEV
                     FDELGLKPKIIAGTSIGALFGAAYASGLPAALIRAHAEQILSQRLDIARLLFAARAEP
                     IMKFFNVLPVRFALLKPEQLLESVLPSRVARNFAQLQIPLRVVATDFYAQEQVVLSQG
                     ALRTAIAASMALPAIFAPVVSEGRTLMDGGLVNPLPFDVIRAEADITVAIDVSGASTG
                     PGKHPQPSAFSALMSSSQILQRSIVREKLKVDQPDIYIDVEVDEFHVLQFHRFRAVLE
                     AAMPAKDKLRRQLLRVLASQTAETLPAAAAPEAEGGRKKRLPRLKRLAQGRRP"
BASE COUNT          311 a          652 c          690 g          342 t
ORIGIN      
        1 cgctcgccac ggcgcaggac cacaaacgcg ccttcgacgg cgatcagggt cccaacaccg
       61 gcggcatggg ggcctactcg ccggctgccg tgatgacgcc gcagctgatc agcgccacca
      121 tggataacat catccggccg acggtacggg ccatggcgga gcgcggcaca cccttcaagg
      181 gtgtgctgtt tgcgggcttg atgctgacgg aggcgggacc caagctgatc gagtacaacg
      241 tgcgcttcgg tgacccggaa tgccaagtgc tgatgccgcg tctcaagtcg gatctgttgg
      301 cggcgctgct ggcaaccgtc gacggcgttc tcggtagctt cgacctgcgc tggcacgagg
      361 atgccgccct gaccgtggtg ctggccgcgc gcggctatcc cggcgcctat gcggtcggca
      421 cggaaatccg tgggctcgag gcggcggccg cgctggaaaa cgtcgaggtg ttccatgccg
      481 gcacggcgcg cgaaggcgca cggctgaccg ccgtcggcgg acgcgtgctc ggcgtgacgg
      541 cgcgcgctgc gaccgtgagc gaggcgcgcc ggcgtgccta tgcggcggtg gcgcggatcg
      601 attggccgga gggcttttat cgcaccgaca tcggttggcg ggcgatggaa cgggagcagc
      661 gcggcgcatg aggctggagc tcgatctttt ccccggctgg gctgagcgtc ggacgccaca
      721 ggcgatgctg gccaccatcc tgcccttgcg cttggcgcag ggaggccggc gatgagcgag
      781 gcgacgcgga ccaacgcgct gacggcgcgc aaaggaggcc cgctcgtgct gcggccaagc
      841 atcgccctgg cgctcggcgg cgggggtgcg cgcggccttg cccacatcct gatgctcgag
      901 gtgttcgacg aactgggcct caaacccaaa atcattgctg gcacctcgat cggcgcgctg
      961 tttggcgccg cctacgcctc cgggctgccg gcggcattga tccgggccca cgccgagcag
     1021 atcttgagcc agcgtctgga tattgcgcgg ctcctatttg cggcccgcgc cgagccgatc
     1081 atgaagtttt tcaacgtgct gccggtgcgc tttgcattgc tgaagccgga gcagctgctc
     1141 gagtccgttt tgccgtcgcg cgtggcgaga aattttgcgc aacttcagat ccccctgcgg
     1201 gtggtcgcca ccgactttta tgcccaggag caggtggtcc tatcgcaggg tgcgctgcgg
     1261 acggcgatcg ccgcgagcat ggcgctgccg gccatttttg ccccggtcgt gagcgaggga
     1321 cgcacgctga tggatggcgg acttgttaat ccactgccct tcgacgtcat tcgcgcagag
     1381 gccgacatca cggttgccat cgatgtgagc ggcgcctcga cgggcccggg caagcacccg
     1441 cagccctccg ccttttcggc cctgatgtcg tcgtcgcaaa tcctgcagcg ctcgatcgtg
     1501 cgcgagaagc tcaaggtcga tcagcccgac atctatatcg acgttgaggt cgatgaattt
     1561 catgtcctgc aatttcatcg ctttagggcc gtgctcgagg ccgccatgcc ggcgaaagac
     1621 aaactgcgcc gccagctgct gcgcgtgctg gcgagccaaa ccgccgagac gttgccggcg
     1681 gcggccgcac cggaggccga gggcggccgc aagaagcgct tgccgcgcct gaaacgcctg
     1741 gcgcaggggc gccggcccta agggccggcg cggccgcggc cggccgcgtg gaaaaatgac
     1801 gccgtgccag gtgattggcc gagcagcctc agtcgagaac tgcacaattg ccacagccgc
     1861 acagcttttt gcctccgttt cgtcgatttg ccggtcttac acgaccgcgc ggcgctagcc
     1921 tcgtcgctcc aggtcttgga agggctaacc ccgtgcgccg cccgcggcag aacgaaggct
     1981 ttcacatcga cgtcg
//
LOCUS       DASTMT010000117         2022 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_5939601, whole genome shotgun sequence.
ACCESSION   DASTMT010000117 DASTMT010000000
VERSION     DASTMT010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2022
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5939601"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            195..764
                     /locus_tag="VFR00_02400"
     CDS             195..764
                     /locus_tag="VFR00_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007564216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEU0158130.1"
                     /translation="MANRERLPPIAADKMTKEQRLAAEEFKAGRGHDLIGPFAVMVRS
                     PEVMLRAKGLGDYLRFRNVLPKRVSEMVILLTAREWTQEFEWSHHYSYALEAGLAPAI
                     VGAIADGRRPQDMAEDEAAAYDFAVELQRRKSVSDATYAEACRVFGEPGVIDLIGVVG
                     YYSLLAMMMNVARTEPDTDKGLPLPRFPD"
     gene            767..1357
                     /locus_tag="VFR00_02405"
     CDS             767..1357
                     /locus_tag="VFR00_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019860693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1285 domain-containing protein"
                     /protein_id="HEU0158131.1"
                     /translation="MAADTGHRIYGLEALLQAQAGKGPPPVEAWNPPYCGDVGLAIRA
                     DGVWEYRGSPIGRASLVKLFASVLRRDADGRHYLVTPAEKVDVRVADAPFLAVEMEVR
                     AAGCAQELIFRTNVDDLVSVGAEHPLRFEVDSASGGLKPYLAVRGRLEALLTRALSFD
                     LAELALAAADGEPRRAGVWSRGSFFALPPTADEPTP"
     gene            1354..2019
                     /locus_tag="VFR00_02410"
     CDS             1354..2019
                     /locus_tag="VFR00_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015668656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HEU0158132.1"
                     /translation="MSGAVGALSEARLRELAGLGLAAEPSPAIFDARTGRAYGPSDWD
                     LNPELKGELVALANPRPAAVLVPIVLREELTVLLTQRSHDMASHPGQISFPGGKLEAG
                     DATAIDCALREAKEEIGLPPGNVEPLGFLDSYRTGTGFQISPVVGLVDPNFAAVLDPR
                     EVLEVFEVPLAFLMDPANHQRASREWRGRQRFYYAMPYQGRYIWGATAGILKNLYERL
                     LLR"
BASE COUNT          337 a          615 c          722 g          348 t
ORIGIN      
        1 gacctgacta tacgctgggc tgcggagaat gcgaaactgt gttggcaaac atggtcagaa
       61 tatgaacagg tgggattggc cgcaattgct gcaagcgcca agataatggg cagttggccc
      121 cgccgggcgg ggtcgggagt atggcaagag ccgcaaaggg ctcggccggg gctggggaaa
      181 ggacggcaaa gttcatggcg aacagggaac ggctgccgcc gattgcggcg gataagatga
      241 ccaaagagca gaggctggcg gcggaggagt tcaaggccgg gcgcggccat gacctgatcg
      301 ggccgttcgc cgtcatggtg cgctcgcccg aagtcatgct gcgcgcgaag ggcctgggcg
      361 actacttacg atttcgtaat gttcttccca aacgcgtgag cgaaatggtg attttgctca
      421 cggcacggga atggacgcag gagttcgaat ggtcccacca ttacagctac gcgcttgaag
      481 cagggctggc cccggcgatt gtcggtgcga ttgccgacgg acgtcgtccc caggacatgg
      541 ccgaggacga ggcggcggcc tacgactttg ccgtggagct gcagcggcgc aagagcgtca
      601 gcgatgcgac ctatgccgag gcctgccggg tgttcggcga accgggcgtg atcgatctga
      661 tcggcgtcgt cggctactac agcctgttgg ccatgatgat gaacgtggca cgcaccgagc
      721 ccgacaccga caaggggctg cccttgccac gctttccgga ttgacgatgg ccgcggacac
      781 agggcaccgc atttatggcc tggaggcgct gctgcaggcg caagccggca aggggccgcc
      841 gccggtggag gcgtggaatc cgccctactg cggtgacgtc ggtctggcta ttcgcgccga
      901 cggcgtgtgg gagtaccgcg gcagcccgat tggccgcgcc agcctcgtca agctttttgc
      961 cagcgtgctg cgccgggatg ccgatgggcg ccactatctc gtgaccccgg cagagaaggt
     1021 cgacgtccgg gtcgcggatg ccccgtttct ggccgtcgag atggaggtgc gcgcggcggg
     1081 ttgcgcccaa gagctgatct tccgcaccaa cgtcgacgac ctcgtcagcg tcggcgccga
     1141 gcatcccttg cgcttcgagg tcgatagcgc gagcggcggc ttgaaaccct acctggcggt
     1201 gcgggggcgc ctcgaggcgc tgctgacgcg ggccttgtcc ttcgacctcg ccgaactggc
     1261 gctggcggcg gccgatggcg agccccggcg tgcgggtgtg tggagccggg gcagcttttt
     1321 cgcgctgccg ccgacggccg acgagccgac gccatgagcg gtgccgttgg agccttgagc
     1381 gaggcgaggt tgcgcgagct ggcgggactg gggctcgccg cggagccctc gccggccatc
     1441 ttcgatgcgc gcacgggccg cgcctacggc ccgagcgatt gggatctcaa ccccgagctc
     1501 aaaggcgaac tcgtggcgct ggcaaaccct cggcccgcgg cggtgctcgt gcccatcgtg
     1561 ttgcgcgagg aattgacggt gctgttgacg cagcgctcgc acgacatggc gagccacccc
     1621 ggccagatct catttcccgg cggcaagctg gaagcgggcg atgccacggc catcgattgt
     1681 gcgttgcgcg aggccaagga agagatcgga ctgccgccgg gcaacgtgga gccgttgggc
     1741 tttctcgata gctatcgcac cggcaccggc tttcagatca gccccgtcgt cggcctggtg
     1801 gatcccaact tcgccgccgt gctcgatccg cgcgaggtgt tggaagtgtt cgaggttccg
     1861 ctcgcttttc tgatggatcc ggccaaccac cagcgcgcct cgcgcgagtg gcgcggccgc
     1921 caacgtttct actatgcgat gccctatcaa gggcggtata tttggggcgc cacggccggc
     1981 atcctcaaaa acctgtacga gagactgttg ctgcgatgat cc
//
LOCUS       DASTMT010000118         2890 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_4002483, whole genome shotgun sequence.
ACCESSION   DASTMT010000118 DASTMT010000000
VERSION     DASTMT010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2890)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2890)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2890
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4002483"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..265
                     /locus_tag="VFR00_02415"
     CDS             <1..265
                     /locus_tag="VFR00_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012112877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0158133.1"
                     /translation="PLALPGIVAGAILCFILAMNAYATPVLLGGPQFKMMAPLVFGQF
                     RLNNWPFGAAVAFVLMTATLLLTASANVLGQRRYRRRLSGAPL"
     gene            287..1663
                     /locus_tag="VFR00_02420"
     CDS             287..1663
                     /locus_tag="VFR00_02420"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU0158134.1"
                     /translation="MASSAKKIVICGAGIAGVAAAYYLAVGADGHDIVLIEEANPLSL
                     TSDKSTEGYRNWWVGPDQAMPDFMNRSIDLMEGIARATGNRIAMNRRGYLFATTRPDT
                     AARFAALAERAEALGSGPVRVHATASATYRPSPEPGFEGAPTGADIITDPSLIRRHFP
                     QLAPDTLAVLHARRAGWLSAQQLGMVLLEGARARGVKLLRAKVVDVRLAGGRVSAVEL
                     ESQAGRQTLQATELVLAAGPMQKGLAALLGIDLPLLAERHQKISFVDRHGAVPRTSPM
                     LIWLDPQYLGFTAEERSALEVEPETRWLLQEFPSGVHARPEGGGERGSLLLLFNTHVE
                     PTEVVFPLPEAPHYQEIALRGAARMVPALAAYVGTGARAFLDGGYYIKTRENRPLIGP
                     LPVEGAYITGAYSGFGVMAGCAGGELLACHVLGGALPSYAPAFQLTRYDDPGYRARLT
                     TWGDGGQL"
     gene            2270..2470
                     /locus_tag="VFR00_02425"
     CDS             2270..2470
                     /locus_tag="VFR00_02425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0158135.1"
                     /translation="MTLLMKKIGGLGLFLIGGLIAAHGGSDGRGWEVFLGLVVAVIGG
                     ALLAAKIMRRNNEPMETLSRRD"
     gene            complement(2654..>2890)
                     /locus_tag="VFR00_02430"
     CDS             complement(2654..>2890)
                     /locus_tag="VFR00_02430"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HEU0158136.1"
                     /translation="VVEGPYRYVRHPIYAGFLLAMLATAITFGTLASYLAVLVGLLAF
                     LMRIAIEEALMLSQFPEAYRAYQAKTAALVPGLF"
BASE COUNT          452 a          925 c         1009 g          504 t
ORIGIN      
        1 gccgctggcg ctgcccggca tcgtcgccgg ggcgatcctg tgctttattc tggccatgaa
       61 tgcctatgcc acgccggtgc tgttgggcgg tccacagttc aaaatgatgg caccactggt
      121 gttcgggcag ttccgtctca acaattggcc gttcggggct gccgtggcgt tcgtgctcat
      181 gacggcgacc ctgctgctga cggcctcggc gaacgtgctg ggtcagcgcc gctatcggcg
      241 ccggctttcc ggcgcacccc tctaggcgag cagggagccg agcgccttgg ccagctcagc
      301 aaaaaagatc gtcatctgcg gcgccggcat cgccggggtg gctgccgcct actacctggc
      361 ggtcggcgcc gacgggcatg acatcgtcct gatcgaggag gcaaatcccc tgtcgctgac
      421 ctcggataag tcgaccgagg gctaccgcaa ctggtgggtc ggccccgacc aggccatgcc
      481 ggacttcatg aatcgcagca tcgacctgat ggaggggatc gcgcgcgcca cgggcaaccg
      541 catcgccatg aaccgccgcg gctacctgtt cgccaccacc aggccggaca cggcggcgcg
      601 cttcgcggcc ttggcggagc gcgcggaggc cttgggcagc ggtcccgtgc gtgtgcacgc
      661 gacggcctcg gccacctacc ggccctcgcc ggagccaggc ttcgaggggg cgcccaccgg
      721 cgccgacatt atcaccgatc cgagcctgat ccgccggcac tttccgcagc ttgcgcccga
      781 tacgctggcg gtgctgcacg cgcgccgcgc cggctggctc agcgcccagc agctgggcat
      841 ggtgctgctg gagggcgcgc gggcgcgcgg ggtgaagctt ttgcgggcca aggtggtgga
      901 cgtgcggctg gcgggtgggc gcgtaagcgc cgtcgagctc gagagccagg cggggcgaca
      961 aacgttgcag gcgaccgaac tcgtgctcgc ggccggtccg atgcagaagg ggctcgcggc
     1021 cctgttaggc atcgacctgc cgctcttggc cgagcgccac cagaagatca gctttgtcga
     1081 tcggcacggg gcggtgccac gcacgtcccc catgctgatt tggctcgatc cgcaatatct
     1141 gggcttcact gccgaggagc gtagcgccct cgaggtcgag ccggagacgc gctggctgct
     1201 gcaggagttt ccctcgggcg tgcatgcccg gcccgagggc ggtggcgagc gcggcagcct
     1261 cttgctgctt ttcaacaccc acgtcgagcc caccgaggtc gtttttcccc tgcccgaggc
     1321 gccgcattat caggagatcg ctctacgcgg tgccgcgcgc atggttccag cgctcgcggc
     1381 ctatgttggc acgggcgcgc gggccttcct cgatggcggc tactacatca agacccgtga
     1441 aaatcgccct ctgatcgggc cgctgccggt cgaaggcgcc tatatcacgg gggcctattc
     1501 gggctttggc gtgatggcgg ggtgcgccgg cggggagctg cttgcgtgcc acgttctggg
     1561 cggcgccctg ccgagctacg ccccggcatt tcagctgacg cgctatgacg atccgggcta
     1621 tcgcgcgcgg ctgacgacat ggggagacgg cgggcagctc taagcgccgc gcgcggagca
     1681 ttgtacctct aatcggaagc ttagccaggc ccgggtgcca ggcgcacaat tttcggcgcg
     1741 agccacgcga cgccaaggtc tgcgttgcgc gggtgcggaa ctttcaggcg acctcgtctt
     1801 tgacagagca tcatgagcca aacacgaagg aacaaacgat gccggttatc ttgtggcttt
     1861 tgggagtgcc gttaagcctg attctcatcc tgctgcttac gggggtgctg cacttctaag
     1921 ggggtcaagc ttgctggcca accggaggcc cgtgaccacc gcgtggcttg cggttgcgcc
     1981 agttctcgtc cgtgcgctct tggcagggcg cccgaaaggc aggggagaag ggcggttgcc
     2041 agtcagcggc tgcagcgtcg cgcctgccga tgcttcgttg ccggctatcg cgacgctggc
     2101 tcgatcgcca tcatagccgc gcacgcgggc tcgatcggcg accagagtat tactgggccg
     2161 atgccggtga gggcgctggc aatcggggta agtcccgata tgggccgggc ggttgcgagc
     2221 ctacaaggtc ttgcacgttg gcccaacccc gcagggaaag gaggtggcaa tgacgctcct
     2281 catgaagaag atcggcggcc tcggcctttt tctgatcggt ggcctaatcg cggcgcatgg
     2341 aggatccgac ggccgtggtt gggaggtctt cctcgggctc gtcgtggcgg tgatcggggg
     2401 cgcgcttttg gcggcaaaga ttatgcgccg caataacgaa cccatggaaa cgctgagccg
     2461 ccgcgactag gccccccgct cgctttcgcg ctttacgcgg cactaatttg cgcagctcga
     2521 gcagccgcgg gcctcatggt gcgcctgtgg cgtcgagcgg tggtcgggcc gccccacgat
     2581 gatccagcac tgcgggacgt cgcgaatgcc gttcgctgcg gcgtcgactt cgaggacgag
     2641 cccgacatct tctctagaag aggccgggaa cgagggctgc ggttttcgcc tgataggccc
     2701 gataggcctc cggaaactgc gacagcatca gggcctcctc gatggcaatg cgcatcagga
     2761 aggcgaggag ccccaccagc accgccaggt agctcgccag cgtgccgaag gtgatcgcgg
     2821 tcgccagcat ggcgaggagg aaaccggcat agatcggatg gcgaacgtaa cgataggggc
     2881 cctcgaccac
//
LOCUS       DASTMT010000119         1720 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_2033843, whole genome shotgun sequence.
ACCESSION   DASTMT010000119 DASTMT010000000
VERSION     DASTMT010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 1720)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1720)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1720
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2033843"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..826
                     /locus_tag="VFR00_02435"
     CDS             <1..826
                     /locus_tag="VFR00_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018458263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEU0158137.1"
                     /translation="TTVSAYGRGGPYSDRVGFDGIGQVMSGAVYMTGEEDQPYRAQVP
                     WVDFGTALHCAFGTMAALMARKATGKGQWVEGALLATAVTLGNALLIEQAVIAPNRVP
                     TGNRGQTSGPVDIFRTKDGFILVQVVGNPLFKRWARLMGEEHWLLDQRFKDDISRGDN
                     GAVISERMRRWAAGYSNAEALALLAEAKIPAGPVLKPQQTLDDPHINAVGFFQALEFP
                     GAPKPAPIAKVPVWLSATPGSIRRRAPILGEHTDQILAGLGYTKAAIAALREKGVI"
     gene            888..>1720
                     /locus_tag="VFR00_02440"
     CDS             888..>1720
                     /locus_tag="VFR00_02440"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEU0158138.1"
                     /translation="MLEPIGNESFPYFGEEHAMLRATLRRFIAERVLPAADAWEEQGF
                     VPRQILTDMGALGLLGMRYAPEYGGSGLDSLANAVLAEELGRSTYGGFAVTVLVHTDM
                     ASPHLYYAGSKAQLERWMRDITAGRTITAIAMTEPDAGSDLASMRTTAVKTRGGYRLN
                     GAKTFITNGVHADLVFVAAKTGEPGRGRHLTMFALEKGTPGFRVTRPLKKQGWLSSDT
                     AELVFEDCFVAEENVLGEEGRGFYQLVKNLQNERIVLAAQALGEAMKALEITLDWVKQ
                     R"
BASE COUNT          298 a          577 c          570 g          275 t
ORIGIN      
        1 caccacggtc tccgcctacg ggcgcggcgg accttacagc gaccgggtcg gcttcgacgg
       61 catcggccag gtcatgtccg gcgccgtcta catgacgggc gaggaggatc agccctaccg
      121 cgcgcaggtg ccctgggtgg acttcggcac ggccttgcac tgcgccttcg gtaccatggc
      181 ggcgctgatg gcgcgtaaag ccaccggcaa gggtcagtgg gtggaagggg ccctgctggc
      241 gaccgccgtg acgctgggca acgcgctcct catcgagcag gccgtgatcg cccccaaccg
      301 cgtgccgacc ggcaatcggg ggcagacgtc gggacccgtc gatattttcc gcaccaagga
      361 cggcttcatc ctggtgcagg tcgtcggcaa cccgctcttc aagcgttggg cccgcctgat
      421 gggcgaggag cactggctgc tcgatcaacg cttcaaggac gacatctcgc gcggcgacaa
      481 cggcgccgtc atcagcgagc gcatgcgccg ctgggcggcg ggttatagca atgccgaggc
      541 gctcgcactg ctcgccgagg cgaagatccc ggccgggccg gtcctcaaac cgcaacagac
      601 cctcgatgat ccccacatca atgcggtggg tttttttcag gccctcgagt ttcccggcgc
      661 gccgaagccg gcgccgattg ccaaggtacc ggtgtggcta tcggcgaccc cgggctcgat
      721 ccgccgccgc gctccgatcc tgggggaaca cacggatcag atcctggccg ggctcggcta
      781 taccaaggcc gccattgcgg cgctgcgcga aaagggcgtg atctgatcga ggccggcgcg
      841 gcgccgggcc cgtctctgcc acgcccgcgt cctcagcagg aggcagcatg ctcgaaccga
      901 tcggcaacga gagctttccc tattttggcg aagagcacgc catgctgcgc gccaccctgc
      961 gccgctttat tgccgagcgg gtgctgcccg cggccgacgc ctgggaagag cagggctttg
     1021 tgccgcggca aattctgacc gacatgggcg cgctcgggtt gctcggcatg cgctacgcgc
     1081 ccgaatacgg cggctccggt ctcgacagct tggccaatgc ggtgttggcc gaggaactcg
     1141 gacgctcgac ctacggcggt tttgcggtca cggtgctggt gcacaccgat atggcctcgc
     1201 cgcacctcta ctatgccggc agcaaggcac aacttgagcg gtggatgcgc gatatcaccg
     1261 ccggccgcac cataacggcg atcgccatga cggagcccga tgcgggctcg gacctggcct
     1321 ccatgcgcac gaccgcggtc aagacccgcg gcggctatcg cctcaacggc gcaaaaacct
     1381 tcattaccaa tggcgtccac gccgacctcg tattcgtcgc cgccaagacc ggcgagccgg
     1441 ggcgcgggcg tcacctcacc atgttcgcgc tggaaaaagg cacgcccggc ttccgcgtca
     1501 cgcgcccgct caagaaacag ggctggttgt cgagcgacac ggcggagctc gtctttgagg
     1561 attgcttcgt ggcggaggaa aatgtcctcg gtgaggaagg ccgaggcttc taccagctcg
     1621 tgaaaaactt gcagaacgag cgcatcgtgc tcgccgccca ggccctggga gaggccatga
     1681 aagcgctgga aatcacgctc gactgggtta agcagcgcaa
//
LOCUS       DASTMT010000120         2172 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_1049858, whole genome shotgun sequence.
ACCESSION   DASTMT010000120 DASTMT010000000
VERSION     DASTMT010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2172
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1049858"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..322)
                     /locus_tag="VFR00_02445"
     CDS             complement(<1..322)
                     /locus_tag="VFR00_02445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEU0158139.1"
                     /translation="MLAPYLLAVVAVAIVVTSFISGLFGMAGGMILLGLLLLFMDVPP
                     AMVLFGIIQLAANGWRSMLWWRHVAWSIVWRFLVGSTAMFLVMRTIAILPSKATIYIG
                     LGLIP"
     gene            638..1231
                     /locus_tag="VFR00_02450"
     CDS             638..1231
                     /locus_tag="VFR00_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009755621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU0158140.1"
                     /translation="MPALSHAIRPATPDDAPALADFVHFASEGLALRVWSNLAGPRGD
                     AWAVGRERARRETGSFSYRNAFVLEADGAAAAGLIGYPLPEQPTPIAPDLPATFVPLQ
                     ELENLAAGSWYVNVLAAYPEHRSHGLGTALLERARAIALKLGRPALSIIVADANLGAR
                     RLYERCGYREAGRRPMLKEDWINAGTHWVLLTREARQ"
     gene            1264..2058
                     /gene="trpC"
                     /locus_tag="VFR00_02455"
     CDS             1264..2058
                     /gene="trpC"
                     /locus_tag="VFR00_02455"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013947224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HEU0158141.1"
                     /translation="MSDILTSIIAYKRAEIAAAQARRPLHEVEIAAHAAPPVRGFAEA
                     LQHQIAGGRFALIAEIKKASPSKGLIRADFDPAGLAHAYEAGGASCLSVLTDQPSFQG
                     APEHLSQARAASQLPVLRKDFMLDRYQVAEARALGADCILIIMAAVDDVVAGTLCAYA
                     KAWNMDAIAEVHDRGELDRALRLDCRMLGINNRDLKTFKTTLETSESLAAGVPDDRIV
                     VAESGIASHADLIRLAKAGVHAFLVGESLMRAADVAAATRSLLGAP"
BASE COUNT          364 a          767 c          716 g          325 t
ORIGIN      
        1 aggggatcag accgagaccg atgtagatcg tggccttgct gggcagaatg gcgatcgtgc
       61 gcatgaccag aaacatggcg gtcgatccca ccaggaagcg ccaaacgatg ctccaggcga
      121 cgtgccgcca ccacagcatg gaacgccagc cattggccgc caactggatg atgccgaaca
      181 gcaccatcgc cgggggcacg tccatgaaca gcaacagcag cccgagcagg atcatgccgc
      241 cggccatgcc gaaaagaccg gaaataaagg atgtgaccac gatcgccacg gcgaccaccg
      301 ccagcaggta gggtgccagc atctagtggc gatccggccg cagcttgccg ccccgcggca
      361 cggcgggccg gcggcctacc gcgcggcagg caaaacgcgc cattgtggtc atgggccgct
      421 cgttcaatgt cgtcgcgctg ccatcaatcg gttggggccg tggctggcgc gaggctaacg
      481 ctaggctggg gccgccacaa cgcgtcacga cgcgcgcgtc ctggctcggc cgttccggcg
      541 atgcggcatg caaattggcc tcgcgccgcg ccatcccagt tgcacaggga gccgcagctg
      601 gcgtaggtgg ggcgaggtca cagcgggacg aacagtgatg ccagccctta gccatgccat
      661 ccggccggcg acgcccgacg acgcgccggc ccttgccgat ttcgtgcatt tcgccagcga
      721 gggtctggcg ttgcgcgtct ggagcaacct cgccggtccg cgtggggacg cctgggccgt
      781 cggtcgcgag cgcgctcggc gcgagacggg cagcttctct tatcgcaacg ccttcgtgct
      841 cgaggccgat ggcgccgccg ccgccgggct gatcggctat cccttgcccg agcaacccac
      901 ccccattgcc ccggatctgc cggccacctt cgtgcccctg caggaactgg aaaatttggc
      961 cgcgggcagc tggtatgtga acgtgctcgc cgcctacccc gagcaccgta gccatggcct
     1021 gggcacggcg ttgctcgagc gcgcccgggc catcgcgctc aagctggggc gacccgctct
     1081 cagcatcatc gtggccgacg ccaatttggg cgcgcgccgg ctctacgagc gctgcggcta
     1141 ccgtgaggcc gggcggcgcc cgatgctgaa agaggactgg atcaatgccg ggacccactg
     1201 ggttttgctg accagggagg ccagacagta gccgcccgca cgccccaagc gtatgtttga
     1261 gccatgagcg atatcctcac cagcatcatt gcctataagc gtgcggaaat tgccgccgcc
     1321 caggcccgcc gccccctgca cgaggtcgag atcgccgccc acgcggcgcc gccggtgcgt
     1381 ggctttgccg aggccctcca acaccagatc gccggcgggc gttttgccct gatcgccgag
     1441 atcaagaagg cgagcccctc caagggcctc atccgcgccg acttcgatcc cgccggcttg
     1501 gcccacgcct acgaagccgg cggcgccagc tgcctatcgg tcctgaccga tcagccctcg
     1561 ttccagggtg cgccggaaca tttgagccag gcgcgcgccg cctcgcagtt gccggtcctg
     1621 cgcaaggatt tcatgctcga ccgctatcaa gtggccgagg cacgagccct cggcgccgac
     1681 tgtatcctga tcatcatggc cgccgtcgat gacgtggtcg ccggtaccct ttgcgcctac
     1741 gccaaggcct ggaacatgga tgccatcgcc gaggtgcacg atcgcggcga gctcgatcgc
     1801 gccctgcggc tcgattgtcg catgctcggc atcaacaatc gcgacctgaa gaccttcaag
     1861 accacgctgg aaaccagtga gagtttggcc gccggcgtcc ccgatgaccg catcgtcgtg
     1921 gccgaaagcg gcattgccag tcacgccgat ctcattcggc tggccaaggc cggcgtgcac
     1981 gcctttctgg tcggcgaaag cctgatgcgt gcggccgatg tcgccgccgc cacgcgcagc
     2041 ctgctgggtg cgccatgagc ccgagcgcgg gaccgccggc cgtgccgcgg cgacgaccgg
     2101 gccgatggca aggcgcgcgc ccggcgcggc ccgcctgcag ccgcggccag ccgatgcagg
     2161 tcgacaatgg cg
//
LOCUS       DASTMT010000121         2484 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_1377811, whole genome shotgun sequence.
ACCESSION   DASTMT010000121 DASTMT010000000
VERSION     DASTMT010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2484)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2484)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2484
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1377811"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            82..297
                     /locus_tag="VFR00_02460"
     CDS             82..297
                     /locus_tag="VFR00_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013945700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HEU0158142.1"
                     /translation="MGLTGGQMQARQAIGFGLTLALLSALLLAGCGVRGSLEKPPGVQ
                     NESNDATPGKPGEPVPHRPSILDPLIR"
     gene            308..1582
                     /gene="lysA"
                     /locus_tag="VFR00_02465"
     CDS             308..1582
                     /gene="lysA"
                     /locus_tag="VFR00_02465"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015596269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEU0158143.1"
                     /translation="MHHFRYVGGVLHAEDVSLTRLAEAVGTPFYCYATATLERHYRVF
                     EEAFRGLNPLICYAIKANSNQAVIATLARLGAGMDVVSEGELRRARAAGVPADRIIFA
                     GVGKTAEEMAYALDQGILGFNVESEPELVALSAIAASRGRKARIAFRVNPDVDAKTHA
                     KISTGRAENKFGIPFAEAPRLYAAAARLPGLEVAGIHMHIGSQVTAAQPFRDAFSLMR
                     ELALELRAQGLAIEHLDIGGGLGVPYRAGSAPPSPQDYAAIVRETLGDLNLKIYLEPG
                     RAIVANAGVLIARVLYAKRGQAKTFTIVDAAMNDLIRPTLYEAYHEIWPVKAAAPGLL
                     PVPQDVVGPVCETGDYLALERDLVPLAAGDLVAFMTAGAYGAVMASTYNTRRLVPEVL
                     VRQDQFAVVRPRPSYAELLAQDRLPEWLREGT"
     gene            1706..2173
                     /locus_tag="VFR00_02470"
     CDS             1706..2173
                     /locus_tag="VFR00_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEU0158144.1"
                     /translation="MPSTQRLLGAALIALASTTASLAQSTLDPAVIAYQLPDQIKWTD
                     NPRAGNRSAILQGDPSKPGPYAMLLQWLPGNMSRPHFHPNDRFFLVVSGTWWVGAGPT
                     FDPNATVPMPAGSSVTHFAKGVHFDGAKDEPATILVWGEGPATSTPFAPPPAK"
     gene            2281..>2484
                     /locus_tag="VFR00_02475"
     CDS             2281..>2484
                     /locus_tag="VFR00_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011501622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="modification methylase"
                     /protein_id="HEU0158145.1"
                     /translation="MSLRQSIRDRVPQGRVPLDRVPHDRVLVGDCLSELAKLPSASVD
                     LVFADPPYNLQLDRELLRPNNTRV"
BASE COUNT          387 a          859 c          794 g          444 t
ORIGIN      
        1 agccttcaga gattgcggct ccagaagcgt tcgtttcggg gccccgacgt gttcgcgggc
       61 tttagcactc gcggagttta aatgggtttg acgggcggcc agatgcaggc gcggcaggca
      121 atcgggttcg gactgacgct cgccctcctg agcgcactcc tccttgctgg ctgtggcgtg
      181 cgcggctcgc tggagaaacc gccaggggtg caaaacgaaa gcaatgatgc cacgcccggc
      241 aagcccggtg agccggtccc ccacagacct tccatcctgg acccgttgat ccgttgacgg
      301 gcagccaatg catcattttc ggtatgttgg tggtgtcctc cacgccgagg atgtgagcct
      361 gacgcggctt gccgaggctg tcggcacccc cttttattgc tacgccacgg ccacgctcga
      421 acgacactat cgcgtcttcg aggaggcctt ccgcgggctc aatcccctca tttgctacgc
      481 catcaaggcc aactccaacc aggccgtgat cgccaccctg gctcggctcg gcgccggcat
      541 ggacgtggtg tcggagggcg agctcaggcg tgcccgcgcc gccggggtgc cagcggacag
      601 gatcatcttc gccggggtcg gcaaaaccgc cgaggagatg gcctatgcgc tcgaccaggg
      661 cattctcggc ttcaacgtcg aatccgaacc cgagctcgtg gccttgagcg ccatcgccgc
      721 gagccggggg cgcaaggcgc gcatcgcctt tcgcgtcaac cccgatgtcg acgccaagac
      781 gcatgccaag atctcgaccg gtcgggcgga gaacaagttc ggcattccct tcgccgaggc
      841 gccgcgcctc tatgctgcgg cggcacgcct gcccggcctc gaggtggccg gcatccatat
      901 gcatatcggc agccaggtca cggcggccca acccttccgc gatgccttta gcttgatgcg
      961 cgagctcgcc ctcgagctgc gtgcgcaggg gttggcgatc gaacacctcg acatcggcgg
     1021 cggcctggga gtgccctatc gtgccggcag cgcaccgcca tcgccgcagg actacgcggc
     1081 catcgtgcgc gagacgctgg gcgatctcaa tctcaagatc tacctggagc ccgggcgggc
     1141 aatcgtcgcc aatgccggcg tgctgatcgc ccgggtgctt tatgcgaagc gcggccaggc
     1201 caagaccttc acgatcgtcg atgccgccat gaacgatctc attcgcccga cgctctatga
     1261 ggcttaccac gagatctggc cggtcaaggc cgccgcgccg gggctcctcc cggtgcctca
     1321 ggatgtggtc gggcccgtgt gcgaaaccgg cgactacctg gcgctggagc gcgacctcgt
     1381 cccgctcgcc gctggcgatc tcgtcgcttt catgacggcc ggcgcctacg gcgcggtgat
     1441 ggcctccact tacaacacgc gccgattggt cccggaggtg ctagtgcgcc aagaccaatt
     1501 tgccgtggtg cggccccgcc ccagctatgc cgagctcttg gcccaggacc gcctgcccga
     1561 gtggctgcgc gagggtacgt gagacggctg tcgctgcccg cagtggcggt tctgcgccgc
     1621 tgccgcgagg ctccgttctg ccggcggtgg ggcgctagcc tgcctgccgc ctccccttgt
     1681 gccaaccaac tcaggaggtc ttcccatgcc aagcacacag cggctcctcg gggcggcgct
     1741 catcgcgctc gcctctacga cggcgagctt ggcgcagtcg acgctcgatc ccgccgttat
     1801 tgcctatcag ctgcccgacc agatcaaatg gacggataat ccgcgcgccg gcaaccgcag
     1861 cgccatcctg cagggtgacc cgagcaagcc cgggccttac gccatgttgt tgcaatggct
     1921 gccgggcaat atgagccgac cgcattttca tcccaacgac cgatttttcc tggtggtgtc
     1981 gggcacctgg tgggtgggcg ccgggcctac cttcgatccc aacgcgaccg tgccgatgcc
     2041 agcgggaagc tcggtgacgc actttgccaa gggcgtgcac ttcgatggcg ccaaagacga
     2101 gccggcaacc attctggttt ggggtgaggg gccggcgacc agcacgccgt tcgcgccgcc
     2161 gcccgccaag tgaggcgccg gcccgccggt aaggttcgcc cggcaacccg tggcactggg
     2221 ctgtgctaag cttggcacgg ccggcggcca acttgtgttt gactggtaag agttgcgtac
     2281 atgtctctgc gtcagtctat ccgcgatcgc gtgcctcagg gccgcgtgcc cctggatcgt
     2341 gtgccccacg atcgcgtcct ggttggcgac tgtctgagcg agctggccaa gctcccctcg
     2401 gccagcgtcg acctcgtctt cgctgaccca ccctataatc tgcaactcga ccgcgagctg
     2461 ctgcgaccca acaacacccg ggtc
//
LOCUS       DASTMT010000122        16864 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_66123, whole genome shotgun sequence.
ACCESSION   DASTMT010000122 DASTMT010000000
VERSION     DASTMT010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 16864)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16864)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16864
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66123"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..329
                     /locus_tag="VFR00_02480"
     CDS             <1..329
                     /locus_tag="VFR00_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009463973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polyphosphate kinase 2"
                     /protein_id="HEU0158146.1"
                     /translation="GREMQLKRFHRRRHDPLRRWKITDIDLAAIAKWDDYTKAKEDMF
                     RFTHTAASPWTVIRANDKRRTRLEAIRSVLYALDYEGKDAAVVDKPDVNIVGSRPDLL
                     YGTRQA"
     gene            complement(739..1503)
                     /locus_tag="VFR00_02485"
     CDS             complement(739..1503)
                     /locus_tag="VFR00_02485"
                     /inference="COORDINATES: protein motif:HMM:NF018473.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1223 domain-containing protein"
                     /protein_id="HEU0158147.1"
                     /translation="MRRLVLLGLAIGFAGGTGVAQPQGAGLATGAAGSPAPAVVELYT
                     SQGCSSCPAADALLAQLAGRGEVVALSLSVDYWDYLGWRDTLANSKFSERQRAYAKAR
                     GDGAIYTPQIVVNGLAHVNGSDEGQILQAIDKTSKAVAAQRVPVRLSEDKGKLIVETG
                     AAPAGTVVKDATLWLAVMSPKVEVSIGRGENQGKKITYTNVVRELIPIGSWSGKPLTV
                     ELQRQAILRPGADRCAVLLQQGHAGPIVGAALMNHF"
     gene            complement(1636..4320)
                     /gene="acnA"
                     /locus_tag="VFR00_02490"
     CDS             complement(1636..4320)
                     /gene="acnA"
                     /locus_tag="VFR00_02490"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018698784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HEU0158148.1"
                     /translation="MSTPSLDSFKSRKTLKVGSTSYSYFSLPDAEAHGLPGLSRLPFS
                     LKVLVENLLRHEDGRSVRAEDIRAVLDWLKAKTSNVEIAYRPARVLMQDFTGVPAVVD
                     LAAMRDAMKALGGDPSKINPLVPVDLVIDHSVQVDFFGSADAFARNVEIEYERNKERY
                     EFLRWGAGAFNNFRVVPPGTGICHQVNLEYLAQSVWTKAGRPELAFPDTCVGTDSHTT
                     MVNALGVLGWGVGGIEAEAAMLGQPVAMVIPEVIGFGFKGKLKEGVTATDLVLTCTQM
                     LRKRGVVEKFVEYFGQGLDHLSVEDRATIANMAPEYGATCGFFPVDADTIKYLKATGR
                     SPERVALVEAYCKAQGLWREGGAEPVFTDTLELDLGSVEPSLAGPKRPQDRVALGRAK
                     ADFAQALPEIVKGADPKLRVPVEGAGYALGHGDVVIAAITSCTNTSNPSVLVAAGLLA
                     RNARARGLKPVPWVKTSLAPGSQVVTDYLAKAGLQEDLDALGFNLAGYGCTTCIGNSG
                     PLAEPIAAAIHAGDLVAAAVLSGNRNFEGRVNADVKANYLASPPLVVAYALAGTLTRD
                     LTSEPLATGSDGRPVYLRDIWPSSAEIAEIVRTCVTPQMFAQRYSAVFEGDAKWRAMG
                     KTRPTLTYKWNSGSTYVQHPPYFENMEPDPPPISDVVDARVLGLFLDSITTDHISPAG
                     SIKRDGPAGVYLIEHQVRPSDFNSYGARRGNHDVMMRGTFANIRIKNMLLGGKEGGLT
                     IHYPSGEQMSIYDAAMRYKLEGVPLVVFAGKEYGTGSSRDWAAKGTRLLGIRAVIARS
                     FERIHRSNLVGMGVLPLVFEEGQSWQELGLTGNEKVSIAGVASLKPRQRLQTTITYAD
                     GTSRQLDVLCRIDTEEELSYYRHGGILPFVLRNLARAA"
     gene            4441..5055
                     /gene="ccmA"
                     /locus_tag="VFR00_02495"
     CDS             4441..5055
                     /gene="ccmA"
                     /locus_tag="VFR00_02495"
                     /EC_number="7.6.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008973379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015439 - ABC-type heme transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC exporter ATP-binding protein CcmA"
                     /protein_id="HEU0158149.1"
                     /translation="MKLIVDKLSVLRDSRPIFAELSCTIEAGSSLILRGPNGAGKTTL
                     LRVIAGLLPASAGGLRVEGAPAETSIGELCHYVGHLNAIKPALTVAENARFWQRFLGG
                     GGQQVEVALAAFGLAGLRDIPAAYLSAGQKRRLTLARVLLAPRAIWLLDEPATALDQD
                     AEAALGAAVAARLAGGGLVIAATHQPLDWPNARDLRLRPAGRAA"
     gene            5052..5720
                     /gene="ccmB"
                     /locus_tag="VFR00_02500"
     CDS             5052..5720
                     /gene="ccmB"
                     /locus_tag="VFR00_02500"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HEU0158150.1"
                     /translation="MNAFCELVRRDVLIAWKEGGTLGVALGFYLVVVVLLPLALGPDL
                     NLIARIAPAVLWVALLLSALLSLGRLFEADFEDGSLDVLATGPLPLEAVVAAKALAHW
                     LTTALPLIALAPVLGLILNLEPQAYGVLVATMLVGTPAISFLGAVGAALTVRTRRGGL
                     LIGLLLLPLFVPTLIFGIEAIGAALAGADSFAASFLLLIAVSLAAIVLAPPAAAAALR
                     FQLQ"
     gene            5780..6538
                     /locus_tag="VFR00_02505"
     CDS             5780..6538
                     /locus_tag="VFR00_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003609648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease"
                     /protein_id="HEU0158151.1"
                     /translation="MQSLTQRLANPGNFMALSARLLPWSWGVSGFLILVGLSLAFFGS
                     PADYQQGQTVKIMYIHVPAAWLAMLIYALIALSSFGLLVFRHPLADVSAKAAAPIGAT
                     FTFLALATGSLWGKPMWGTFWVWDPRLTSVLILFFLYLGLIALRSSIEEEVLAAKLTA
                     VLSLVGIAILPVIKFSVEWNSLHQPASVMRLGAPAIHASLLRPLLVMALGMTALFVAL
                     QLKAMRNEILRRRVKALRRIAAASAADLSQQPAE"
     gene            6543..6689
                     /gene="ccmD"
                     /locus_tag="VFR00_02510"
     CDS             6543..6689
                     /gene="ccmD"
                     /locus_tag="VFR00_02510"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HEU0158152.1"
                     /translation="MDLGPHAAYIWSAYAIVAVTLAALIAWLVSDGRRQRRLIDEIEA
                     RNRR"
     gene            6716..7312
                     /locus_tag="VFR00_02515"
     CDS             6716..7312
                     /locus_tag="VFR00_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013167573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbE family thiol:disulfide interchange protein"
                     /protein_id="HEU0158153.1"
                     /translation="MAPELARQADGSGRSPRLKLWPLAIFVVLALLFAFALRSGDPAK
                     LPSALIGKPVPQLSFAALDGLMEGTRPVPGFAAAELAAGKVTVVNFWASWCVPCVQEH
                     PVLGLITQRTGTPVFGVNYKDQTSQARRFLGRYGNPFTAVGVDADGRGAIEWGVYGMP
                     ETFIISGKGEIVYKHVGPLSAESLETKILPAIRAAEAH"
     gene            complement(7290..8474)
                     /gene="nhaA"
                     /locus_tag="VFR00_02520"
     CDS             complement(7290..8474)
                     /gene="nhaA"
                     /locus_tag="VFR00_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011505246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HEU0158154.1"
                     /translation="MTSERRLREGALAFVQHEATAGLLLLGATLAALLVSNSSLRSLY
                     QSVLSAPAGLRLADFSLEKPVLLWINDGLMAIFFLLVGLEIKRELIVGELASPGKAAL
                     PGIAAVGGMAVPALIYSLINAGDWVALRGWAIPAATDIAFAVGVLALLGNRVPASLKI
                     FLLALAILDDLGSILIIAVFYTGDLHWSALLLASLAAGALAALNRGGIRRLAPYLLIG
                     LFIWLCVLKSGVHATLAGVVVAMAVPLRGGAPQQTSPLESLEARLHPWIAYLILPLFA
                     FANAGVSLKGLSLAKLTAPVPLGIALGLLVGKPVGIFAASWLAVASGLAPRPEGANWR
                     QLLGIGMLGGIGFTMSLFIGTLAFPDPAHAAQLRLGVLTGSLLSAIAGYCVLRFSAPR
                     RP"
     gene            complement(8471..9277)
                     /locus_tag="VFR00_02525"
     CDS             complement(8471..9277)
                     /locus_tag="VFR00_02525"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: protein motif:HMM:NF020132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEU0158155.1"
                     /translation="MGRVLALSSQVARGHVGLAATVPALGALGQEVWALPTVILASRP
                     GLGRLAKYPLPAADLDALLAALEADGCWGELDAVFTGYFPSAAAVTSAAQAIARLKRA
                     NPGVTICVDPILGDADRLYVAADTAAAIRDELLGLATLATPNLFELAWLTGAGQLNAQ
                     DVGELEVVAAARRLGVPTVVVTSARETPEEIATALLCPAGVFWRTLPKHRGIPNGAGD
                     LFAGLLLGYLLRGQATPAAFAASLADLDVVLAASTGRSSLVLTALKPPPS"
     gene            9410..10297
                     /locus_tag="VFR00_02530"
     CDS             9410..10297
                     /locus_tag="VFR00_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013950193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HEU0158156.1"
                     /translation="MQTTPRRSVLYMPGSNARALEKAKSIAADALILDLEDAVAPAAK
                     ASARTQVCAAVHSGGYGRRELVIRINALETAWGEADLAAALAVAPDAILLPKTGSAAD
                     VLAVTDRLARAEAAAKTRLWVMIETPLAILNLKEIAAAAARERARLDCLVLGTNDLVK
                     ETRADLSAGAWPAHYWLSAAVVAARAFGLDVLDGVYNDFRDREGFRRACLDGRALGFD
                     GKTLIHPDQVSDANAIFAPVEAELAFARKVLAAFQQPENAGKGVITVEGRMVELLHAK
                     MAARTVAIAEAIQALGTDR"
     gene            10294..11367
                     /locus_tag="VFR00_02535"
     CDS             10294..11367
                     /locus_tag="VFR00_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014889836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HEU0158157.1"
                     /translation="MTANKATAPKTNAGNFFEDFRLGQVFEHATPRTLTVGDVALYHG
                     LYGARFAVQSSEPFAQAIGFPRAPLDDLLVFHVVFGKSVPDISLNAIANLGYADCRFL
                     EPVYPGDTLNAASEVIGLKENSNRKSGVVYVRTTGRKTSGESVLRFVRWVMVRKRDPA
                     APAPAPVIPELAQGVDPAGLAQHLPRLDPSRYAYALAGSPYRWGDYRVGEKIDHAEAM
                     TLEEAEHQIATRLYQNTARVHFNQHAQAQGPFKRRLVYGGIVLSLARALSFNGLANAF
                     HIAAINGGRHVAPTFAGDTLYAWSEILQVAEVPLRPDIGALRTRLVAVKNRSSADFPL
                     QTPQGEDADGVVLDLDLWLVLPR"
     gene            11523..11930
                     /gene="sdhC"
                     /locus_tag="VFR00_02540"
     CDS             11523..11930
                     /gene="sdhC"
                     /locus_tag="VFR00_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015315036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HEU0158158.1"
                     /translation="MADTGESTARPLSPHLSVYRPLINMVMSILHRLTGAALYVGTLL
                     LAAWLIAAASGEAQFARINSLFAHPLGRLVLFGYSWALLHHMLGGVRHLVWDTGRGFS
                     IAQVNALSWLTIIGSVLLTLALWMVGLTLRGAL"
     gene            11932..12315
                     /gene="sdhD"
                     /locus_tag="VFR00_02545"
     CDS             11932..12315
                     /gene="sdhD"
                     /locus_tag="VFR00_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013950198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, hydrophobic membrane
                     anchor protein"
                     /protein_id="HEU0158159.1"
                     /translation="MVGYRTPLARVRGLGSAKDGTEHFWKQRLTAVANLILISFAIYL
                     VVRLAGADYATVKKTLARPQNAIPLLLLILSGVIHMRLGMQNIIEDYVHAEGYKVAAL
                     LLNSFFTALVGLVCAYAVLKLSFGA"
     gene            12319..14166
                     /gene="sdhA"
                     /locus_tag="VFR00_02550"
     CDS             12319..14166
                     /gene="sdhA"
                     /locus_tag="VFR00_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013417790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HEU0158160.1"
                     /translation="MPKSTLDKGNGITKPALNGKAYPLTDHVYDVVIVGAGGAGLRAT
                     LGCAVAGLKTACISKVFPTRSHTVAAQGGVAAALGNMGPDDWRWHMYDTVKGADWLGD
                     QDAIEYLCRNAPEAVYELEHYGVPFSRTEDGRIYQRPFGGMTTHYGEGPPAQRTAAAA
                     DRTGHAMLHTLYGQSLRHAAEFFIEYFAIDLIMDQDGGCHGVVALKLDDGTLHRFRAK
                     KTILATGGYGRAYFTCTSAHTCTGDGNAMVLRAGLPLEDMEFVQFHPTGVYGAGVLIT
                     EGARGEGGYLTNASGERFMERYAPHVKDLASRDVVSRAMTIEIREGRGVGPDKDHIFL
                     HLDHLDPKILHERLPGITESAKVFAGVDLRREPIPVLPTVHYNMGGIPTNYHGEMLTL
                     KGGNPDYVVPGLMALGEAACVSVHGANRLGSNSLIDLVVFGRAAGLQCAKTIEPGGTH
                     PDLPADAGELALARLDRFRHASGGTPTAALRLRMQRIMQMNCSVFRDGPVLKEGLAKI
                     TDVWKGVSDIRVSDRSLIWNSDLVETLEFDNLIAQAAVTVAAAANRTESRGGHAREDY
                     PERDDENWMKHTLTWADDRTQSVRLDYRPVHAYTLTNEVNYFPPKPRVY"
     gene            14226..15470
                     /locus_tag="VFR00_02555"
     CDS             14226..15470
                     /locus_tag="VFR00_02555"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEU0158161.1"
                     /translation="MAGTRPLRIAIAGAGTAGLAAAAFLARAGHDVRVFERFDRPRPV
                     GAGLLLQPTGLACLAQLGLDEAALAYGQAIHSIYGETRGGRPILDISYHELGSHLFGL
                     GIHRGALFSVLHAEVLRLGIPLTTTTEIAASRATAGGRILVDRHKYEHGVFDLVVDAT
                     GVRSPLRADAASLRRDRPYAYGAVWGSLLQPADWPHQDRLLQRYDGCHSMVGLLPIGR
                     TPDDARRLVALFWSLRVTDFPAWRAAGLEAWKSRLVEVCPEAKPFLAQLTSLDAMTLA
                     SYADIALGRRRGSRIVSIGDAGRALSPQLGQGANLALIDAATLAQCLAETSLEAALDA
                     YRARRRRHSNFYALASRWLTPFFQSESRWAARLRDLVFPVLGQVPYLRRQMASTLAGM
                     KTGVVTQVDPGQWHPSYALRRQ"
     gene            complement(15577..16755)
                     /locus_tag="VFR00_02560"
     CDS             complement(15577..16755)
                     /locus_tag="VFR00_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008597143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HEU0158162.1"
                     /translation="MRAKSNLSFRGWRRDNDRVGVRNHVIILPLDDLSNAACEAVANN
                     IKGTLALPHAYGRLQFGADLDLHFRTLIGTGSNPNVAAAVVIGIEDGWTKRVVDGIAK
                     SGKPVVGFGIEGHGDIATIAMASHKAKEFLQWASELEREECAIDELWVSTKCGESDTT
                     TGLASCPTVGNMYDKLIPKGIYGCFGETSEITGAEHIARNRAASAKIGDKWMKTFQAY
                     QAEVIEAHKVDDLSESQPTKGNIAGGLTTIEEKALGNLEKIGRKCRYIDVLEPAQTPA
                     AGPGLYYMDTSSAAAECVTLMAAGGYVVHTFPTGQGNIIGNPIVPVIKISGNPKTVRT
                     MPEHIDLDVSGILRRELTLNTAGDALIDMIVRTANGRLTAAEALGHREFVMTKLYRSA
                     "
BASE COUNT         2887 a         5583 c         5398 g         2996 t
ORIGIN      
        1 tcggccgaga aatgcaactg aagcgctttc accgccgccg ccatgacccc ctgcgccgct
       61 ggaagatcac cgacattgat ctggcggcga tcgccaaatg ggacgattac accaaggcca
      121 aggaggacat gttccgcttc acccatacgg cagcttcgcc ctggacggtg attcgcgcca
      181 acgacaagcg ccgcacgcgg ctggaggcga tccgcagcgt gctctacgcc ttggactacg
      241 agggcaaaga cgccgcggtg gtcgacaagc ccgacgtcaa catcgtcggc tcgcgaccag
      301 accttctcta cggcacgcgt caggcgtgat ggcgcaagag cccgctgcgg tagtcggttg
      361 cgcgcgaagg ctgataagtt gcgcgaacgg acgaagggcg agggctgatg ttgcgggcgc
      421 ggaagaatgg ccgcggcgcg ctgccgcggc gtgaaaaaaa tctcggtttt gtcaaaattg
      481 cacacgggct ctgccgtatt tcggcgggca ccggacaatc atctgccgca atgattcggc
      541 atcttgcgac cattgcctct cggctcgatc cacgtcgagg ctacggacac tagcccccca
      601 gccccccggg cctcgccgct gggtcgagcc aaagcaaaaa gaagcaacga ggcaccagga
      661 aaaaggcatc aactgcaacg gccggctttc gccggccgat attttttccg ctggctgctt
      721 ttagcgggcc tggccgcatt agaaatggtt catcagagcg gcgccgacga tgggccccgc
      781 atggccctgc tgcagcagga cggcgcaacg gtcggcgccc ggccgcaaga tcgcctgacg
      841 ctggagctcg accgtcaaag gcttgccgct ccacgagccg atcgggatca gctcgcgcac
      901 cacgttggtg taggtgattt tcttgccctg attttcgccg cggccgatag agacctctac
      961 cttgggcgac atgacggcga gccaaagcgt cgcgtccttc acgacggtgc ccgccggcgc
     1021 cgcgccggtt tcgacgatca gcttaccctt gtcctcgctg aggcggacgg gcacgcgctg
     1081 ggccgcgacc gccttgctcg tcttatcgat ggcctgcaaa atttggcctt cgtcgctgcc
     1141 attgacgtgc gcgagaccat tgaccacgat ttgcggtgtg tagattgccc cgtcgccacg
     1201 cgctttggcg taggcccgct ggcgttcgct gaacttggaa ttcgcgagcg tgtcgcgcca
     1261 gcccagatag tcccaatagt cgaccgacag cgataaggcg accacctcgc cccggcccgc
     1321 cagctgcgcc aacagggcat cggcagcggg acacgacgag cagccttggc tggtgtagag
     1381 ctccaccacc gccggtgctg gcgagccggc cgcaccggtc gccaggccgg ccccctgggg
     1441 ttgagcgaca cccgtcccgc cggcaaaacc aatggccagg ccgaggagca caagacgtcg
     1501 catgaggcaa tgccatcctt tttttagcac gccaagttgc tgcactatcg gcatttgttt
     1561 cttcaatagc gagtcacgaa tgggtgagct tacccggtgc gttccgcata ccgccacgct
     1621 cagttaacgg atcggtcagg cggcgcgggc gaggttgcgc aacacgaacg gcagaatgcc
     1681 gccgtgccgg taatagctga gctcttcctc cgtatctatg cggcacaaca cgtcgagctg
     1741 ccgcgacgtt ccgtcggcgt aggttatggt tgtctgcaat ctctgccgcg gtttcaggct
     1801 cgccacgccg gcgatggaaa ctttctcgtt cccggttagt cccagctcct gccaggactg
     1861 accctcttcg aacacgaggg gcagcacgcc catgcccacg aggttcgagc ggtgaatgcg
     1921 ctcaaaagag cgcgcgatca cggcgcgaat cccgagcaag cgggtaccct tagccgccca
     1981 atcccggctc gaacccgtgc cgtattcctt gcccgcgaac accaccaaag gcacgccttc
     2041 caacttgtag cgcatggccg cgtcgtaaat cgacatctgc tcaccggacg gatagtggat
     2101 cgtcaggcca ccctccttgc cccccagcag catgttcttg atgcggatgt tagcgaacgt
     2161 gccgcgcatc atgacgtcgt gattgccgcg gcgggcgcca tacgaattga agtccgacgg
     2221 gcgcacttga tgttcgatca aatagacgcc ggctggacca tcgcgcttga tcgagccggc
     2281 cggcgaaatg tggtccgtgg tgatgctgtc gaggaacagg ccgagcacgc gcgcatcgac
     2341 gacatcgctg atcgggggcg gatcgggctc catgttctcg aagtacggag gatgctgcac
     2401 gtaggtcgaa ccggaattcc atttgtaggt gagcgtcggc cgcgtcttac ccatcgcccg
     2461 ccattttgca tcgccctcga acaccgcgct gtagcgctgc gcgaacatct gcggcgtcac
     2521 acaggtgcga acgatctcgg cgatctccgc cgaactcggc cagatatccc tcagatagac
     2581 gggccgaccg tcgctgcccg tggccagcgg ttccgaggtg aggtcgcggg tgagcgtgcc
     2641 ggcgagcgca taggccacga ccaggggcgg cgaggccagg taattggcct tcacgtcggc
     2701 attgacgcgg ccttcaaaat tgcggttgcc ggacagcacg gcggccgcca ccagatcgcc
     2761 ggcgtgaatg gcggccgcaa tcggctccgc cagcggcccc gagttgccaa tgcaggtggt
     2821 gcagccgtag cccgccaggt tgaatcccag ggcgtcgagg tcctcctgca ggccggcttt
     2881 ggcgagatag tcggtgacga cctgcgagcc gggcgcaagc gaggtcttga cccagggcac
     2941 gggcttgaga cccctggcgc gggcgttgcg cgccagcagc ccagcggcca ccagcaccga
     3001 agggttggag gtattggtgc acgatgtgat ggcggcgatg acgacgtcgc cgtgaccgag
     3061 cgcgtagccg gcgccctcga cgggaacgcg cagcttggga tcggcaccct tgacgatttc
     3121 cggcaacgct tgggcgaagt cggccttggc gcggccgagc gcgacgcggt cttgcggccg
     3181 tttgggtccg gcgagcgagg gctcgacact gcccaaatca agctccaagg tgtcggtgaa
     3241 cacgggttcg gcgccgcctt cgcgccatag gccctgcgcc ttgcagtagg cctccacgag
     3301 cgcaacccgc tccgggctgc gtccggtcgc tttcaggtac ttgatggtat cggcgtcgac
     3361 gggaaagaac ccgcaggtgg cgccgtactc cggcgccatg ttggctatgg tggcgcgatc
     3421 ctcaaccgag agatggtcga ggccttggcc gaagtattcc acgaatttct cgacgacccc
     3481 gcgcttgcgc aacatctggg tgcaggtgag cacgaggtcg gtcgcggtga ccccttcctt
     3541 cagcttgccc ttgaagccaa agccgatcac ctcagggatc accatggcga cgggctgacc
     3601 gagcatggcg gcctccgcct cgatgccgcc cacgccccag ccgagcacgc ccagcgcatt
     3661 gaccatggtg gtgtggctgt cggtgccaac gcaggtatcg ggaaacgcca gttccggccg
     3721 acccgccttg gtccacacgc tctgggccag atactcgagg ttgacctgat ggcaaatccc
     3781 ggtgccgggc ggcaccacgc ggaagttgtt gaaggcgccc gccccccagc gcaagaactc
     3841 gtagcgctcc ttgttgcgct catactcgat ctcgacattc ctggcgaacg catcggccga
     3901 gccgaagaag tccacctgca cggaatgatc gatcacgaga tcgacgggca ccagtggatt
     3961 gatcttggaa ggatcgccgc ccagcgcctt catggcatca cgcatggcgg cgagatcgac
     4021 gaccgcgggc accccggtga agtcctgcat cagcacgcgc gcggggcgat aggcaatctc
     4081 gacgttggag gttttcgcct tgagccaatc cagtacggcg cgaatgtctt ccgccctcac
     4141 cgagcggcca tcctcgtgcc gtagcaggtt ttccaccagg accttgagcg agaatggcag
     4201 ccgcgagaga cccggcaaac catgcgcctc ggcatcgggg agggaaaaat agctgtagga
     4261 cgtcgagccg accttaaggg tcttgcggct tttgaagctg tcgagggaag gggtgctcat
     4321 gcgcgcatct cctagctacg ggccgcgcct gttgaaagcg cgttttgacc tggcctggag
     4381 gcaaaccagg gccctatata aaacggttct aatatgcgcg ttcgggccgg ggaagccctt
     4441 atgaaactga tcgtcgacaa actgtccgtg ctgcgggaca gccgaccgat cttcgccgag
     4501 ctgtcctgca cgatcgaggc cggctcgagc ctgatcctgc gcggcccgaa cggggccggt
     4561 aagaccacgc tgctgcgcgt gattgccggc ctcttgccgg cgagcgccgg tgggctgcgc
     4621 gtggagggtg ccccggccga gaccagcatc ggcgagctct gccactacgt gggtcacctc
     4681 aacgcgatca agccggcgct gaccgtggcc gagaacgcgc ggttctggca acgctttctg
     4741 ggtggcggcg gccagcaggt cgaggtcgcg ctcgcggcat ttggcctggc gggattgcgc
     4801 gacattccgg ccgcctacct gtcggccggg caaaaacgcc gcctgacgct tgcccgcgtg
     4861 ctgttggcac cgcgcgccat ctggctcctg gacgaaccgg cgacggccct ggaccaggac
     4921 gcggaggcag ccctcggggc ggcggtggcg gctcggctcg caggcggcgg cctcgtcatt
     4981 gccgccacac accagccact cgattggccg aatgcgcgcg acctgcggct taggccagcc
     5041 ggtcgtgccg catgaacgct ttttgcgagc tcgtacgtcg cgatgtcctg atcgcgtgga
     5101 aggaaggggg cacgcttggc gtggcgcttg ggttctacct cgtcgtcgtg gtgctgctgc
     5161 cactggcgct gggtcccgac ctcaatttga tcgcccgcat cgcccccgcc gtgctgtggg
     5221 tcgctctcct gctctccgcc ttgctgtcgc tcgggcgctt gttcgaagcc gatttcgagg
     5281 atggctccct cgatgtgctg gctaccggac cgctgccgct ggaggccgtt gtggcggcca
     5341 aagcgttggc gcattggctg acgacggcct tgccgctcat cgccttggct cccgtgctcg
     5401 gcctcatcct caatctggaa ccgcaggcct atggcgtgct ggtcgcaacg atgctggtcg
     5461 gtacgcccgc catcagcttc ctcggcgcgg tcggcgcggc actgacggtg cgaacgcgcc
     5521 ggggtggctt gctgatcggg ctcctgctgt tgccgctgtt cgtgccaacg ttgatcttcg
     5581 ggatcgaggc cattggcgcc gcgctggcag gcgcggacag tttcgcggcc tcatttttgc
     5641 tcctgatcgc cgtgtccttg gcagcgatcg tgcttgcccc gccggcggcg gcggcggccc
     5701 tgcgcttcca gttgcaatga cgaggccgag atcgcggacg aattgcgctc agcaccacaa
     5761 aggcttagat gctaggccca tgcaaagcct cacccaacgg ctcgccaatc ccggcaactt
     5821 tatggcgcta tcggcgcgcc tcttgccgtg gtcttggggc gtcagcgggt ttctcattct
     5881 ggtcggtctg tcgctggcgt ttttcggctc gccggcggat taccagcagg ggcaaaccgt
     5941 caagatcatg tacatccatg tgcccgcagc ctggctggcg atgctgatct atgccctgat
     6001 cgcgctctcc agctttggtc tgctggtttt ccggcacccc ctcgccgatg tctcggcgaa
     6061 ggcggcagca ccgatcggag ccaccttcac ctttctggct ctggccactg gctcgctttg
     6121 gggcaagccg atgtggggaa cgttttgggt gtgggacccg cgtctcacct cggtgctgat
     6181 cctgttcttc ctctatcttg gactgatcgc gctgcgctcc tcgatcgagg aggaggtgct
     6241 ggccgccaaa ctgacggccg tgctcagcct ggtcggcatc gccatcctgc ccgtgatcaa
     6301 gttctcggtg gaatggaact cgctgcatca gcccgccagc gtcatgcggc tcggcgcgcc
     6361 agcgatccat gcctcactgt tgcgaccgct cctcgtcatg gccttaggca tgacggcgct
     6421 gttcgtggcc ttgcagctca aggccatgcg caatgagatc ctgcgccgcc gcgtgaaggc
     6481 gctccgtcgc atcgccgccg ccagtgcggc cgatctgtcg caacagcccg ccgaatgatg
     6541 ccatggattt gggcccacac gccgcttaca tctggtcggc ctacgcgatc gttgccgtca
     6601 cgcttgcggc cctgatcgct tggctcgtca gcgatgggcg ccgccagcgg cgcttgatcg
     6661 acgagatcga ggcgcgaaac cggcgttaaa gacgccgcaa caacgctaag ggacgatggc
     6721 accagagctg gcgcgacagg ccgacggcag cgggcgctcc ccgcgcctca agctgtggcc
     6781 gctggcgatt ttcgtcgtgc tggcgctgct gtttgccttt gccctgcgct ccggcgatcc
     6841 cgccaagtta ccctcggcgc tgatcggcaa acccgtgccg cagttgagct ttgccgccct
     6901 cgacggcctc atggagggca cgcggccggt acccggcttt gctgccgccg agctcgccgc
     6961 cggcaaggtc acggtcgtca atttctgggc ctcctggtgc gtgccgtgcg tgcaggagca
     7021 cccggtgctg ggcctcatca cgcaaaggac gggcacgccc gtcttcggcg tgaactacaa
     7081 ggaccagacg tcgcaagcgc gtcgtttcct cggccgctac ggcaaccctt ttaccgcggt
     7141 aggggtcgac gccgacggcc gcggtgcgat cgagtggggc gtctacggca tgccggaaac
     7201 cttcatcatc agcggcaagg gcgagatcgt ctacaaacac gtcggcccgc tgagcgctga
     7261 gagcctggaa acgaagatac tgcccgcgat cagggccgcc gaggcgcact gaagcgcagc
     7321 acgcagtagc cggcgatggc cgataacagt gatcccgtca gaacaccgag ccgcagttgg
     7381 gccgcgtgtg ccggatcggg gaaagccagc gtgccgatga aaaggctcat ggtgaaaccg
     7441 atcccgccca gcataccgat acctagtagc tggcgccaat tggcgccctc ggggcggggc
     7501 gccaagccgc tggccacggc cagccaactc gccgcgaaga taccaacggg cttgccaacc
     7561 aggagaccca aggcgatgcc gaggggtacg ggcgccgtca gcttggcaag gctgagcccc
     7621 ttgagcgaga cgccggcatt ggcaaaggca aagaggggca agatcagata cgcaatccag
     7681 ggatggaggc gtgcctccag gctttcgagc ggagacgtct gctgtggcgc acctcccctc
     7741 agtggcacgg ccatagcgac cacgacgcct gccaaagtgg cgtgaacgcc ggacttcaag
     7801 acgcacagcc aaataaaaag gccgatcagc aagtaggggg cgaggcgcct gatgccgccg
     7861 cggttcaggg cggccagcgc gccggccgcg agcgaggcca acagcagcgc gctccaatgc
     7921 aggtcaccgg tatagaacac ggcgatgatg agaatactgc cgaggtcatc cagaatggcg
     7981 agcgccagca ggaagatttt caacgaggct ggcaccctgt tgccgagtaa ggccaacacc
     8041 cccacggcga aggcgatatc ggtcgccgcc gggatcgccc aaccccgcaa ggcaacccaa
     8101 tccccggcat tgatgaggct gtagatgagc gccggcacgg ccatgcctcc gacggcggca
     8161 atgccgggca gggccgcttt gcccggacta gcgagttcac cgacgatcag ctcgcgcttg
     8221 atctcgaggc cgacaagcaa aaagaagatc gccatcagcc catcattgat ccaaagcagc
     8281 accggctttt ccaggctgaa gtcggcaagg cgcagcccag ccggcgccga aagtacgctc
     8341 tggtagagcg aacgcagcga ggagttgctg accagcaaag cggcgagcgt ggcccccaac
     8401 agcaggagcc cagcggtggc ttcgtgctgg acgaaggcca gcgcaccctc gcgcaagcgc
     8461 cgctcactcg tcatgacggc ggcggcttaa gcgcggtgag caccaagctg gacctgcccg
     8521 tgctcgccgc cagcaccaca tcgagatcgg ctaggctcgc agcgaaggcg gccggcgtcg
     8581 cttgacctcg caacaggtac ccgagcagca gcccggcaaa gaggtcgcct gcgccattgg
     8641 gaatgccgcg gtgcttgggc agtgtgcgcc agaagacgcc ggccgggcag agcaaggccg
     8701 tggcgatttc ctcgggcgtc tcgcgtgcag aggttaccac tacggtcggc acgccgagcc
     8761 ggcgcgcggc cgcaaccacc tccagttctc ccacatcctg ggcgtttagc tggcccgctc
     8821 ccgtcagcca agcgagttcg aagaggttgg gggtcgccag ggtcgctaac cccaagagct
     8881 catcgcgtat ggcggccgcg gtgtccgctg ccacataaag acggtccgca tcccccagga
     8941 tagggtcaac gcagatagtg accccaggat tggcccgctt caggcgcgcg atggcttggg
     9001 cggccgaggt gaccgcggcg gctgagggaa agtaacccgt aaacaccgca tcgagctcgc
     9061 cccagcagcc atccgcctcc agggcggcca gcagggcgtc gagatcggcg gcaggtaggg
     9121 gatatttcgc gagccggccc aggcccggcc gactggcgag aatgacggtg ggcaaggccc
     9181 acacctcctg ccccagggcg ccgagggcag gcacggtggc ggcgaggcca acgtgcccgc
     9241 gggcgacctg cgaggaaagg gcaagaacgc gtcccatgcg tcctcatagc atgatcgcgg
     9301 cccctccata gcgctcccgg ctcgcgggcg cgccgacgtt cacgctggcg gccgcggcag
     9361 gctttgccta ccctggtcga gggctcacta tgttcggaaa aggcgtcaga tgcagactac
     9421 tccgcgccgc agcgtccttt acatgcccgg ttccaacgcc cgtgccttgg agaaggccaa
     9481 atcgatcgcg gccgatgcct tgatcctcga tttggaggat gcggtcgcgc ccgccgccaa
     9541 ggccagcgcg cgcacgcagg tgtgcgcggc cgtccatagc ggaggctacg ggcgccgcga
     9601 gctcgttatc cgcatcaatg cgctcgaaac ggcctgggga gaggccgacc tggcggccgc
     9661 gctcgcggtc gcgcccgacg ccatcctgtt gccgaagacc ggcagcgccg ccgacgtgct
     9721 cgccgtcacg gataggctgg cgcgcgccga ggccgccgcc aaaacacgcc tgtgggtcat
     9781 gatcgaaacg ccattggcga tcctcaatct caaggagatt gcggcggcag ctgcgcgcga
     9841 gcgcgcgcgg ctcgactgcc tggtgctggg caccaacgac ctcgtcaagg agacgcgcgc
     9901 ggatctgagc gccggtgcct ggccggccca ctactggctg tcggctgcgg tcgtggcggc
     9961 gcgggccttc ggcctcgacg tgctcgatgg agtttacaac gacttcaggg atcgggaggg
    10021 ctttcgccgc gcctgcctcg acgggcgcgc gctcggcttc gatggcaaga ccttgatcca
    10081 tcccgaccag gtgtccgacg ccaacgccat tttcgcgccc gttgaggccg agctcgcctt
    10141 cgcgcgcaaa gtcctcgctg cctttcagca gccagaaaat gcgggcaaag gcgtcatcac
    10201 ggtggagggc cgcatggtgg agctgctcca cgccaagatg gccgcgcgca ccgtcgccat
    10261 tgcggaggcc attcaagcgc tgggcaccga ccgatgaccg ccaacaaagc cacggcaccc
    10321 aagaccaatg ccggcaactt cttcgaggac ttccggcttg ggcaagtctt cgaacatgcc
    10381 accccgcgca ctctcacggt cggcgatgtc gccctctacc atggcctcta tggcgcgcgc
    10441 tttgccgtgc aatcctccga gccctttgcc caagcgatcg gcttcccgcg ggcgccgctc
    10501 gacgatctgc tggttttcca tgtcgtgttc ggcaagagcg ttcctgatat ttcgctcaac
    10561 gccattgcca atctcggcta cgccgattgt cgctttctcg aaccggttta ccccggtgac
    10621 acgctcaacg ccgcctcgga ggtgatcggt cttaaggaaa attccaaccg caaatccggc
    10681 gtcgtgtacg tccgcacgac gggccgcaag acgagcggcg aaagcgtgct tcggttcgtg
    10741 cgctgggtca tggtgcgcaa acgcgacccc gcagcgccgg caccggcgcc agtcatacct
    10801 gagctggctc aaggggtcga tccggccggc ctggcgcagc acctgccgcg cctcgacccc
    10861 tcgcgctacg cctacgcgct ggcgggcagc ccttatcgct ggggcgacta tcgcgtcggc
    10921 gaaaagatcg atcacgccga ggccatgaca ctcgaggagg cggagcatca gatcgccacc
    10981 cgcctctatc agaacaccgc cagagtgcac ttcaatcaac atgcacaggc acaagggccc
    11041 ttcaagcgcc gccttgtcta tggcggcatc gtgctttcgc tcgcccgggc gctgtccttt
    11101 aatggcctgg ccaacgcctt tcatatcgcc gccatcaatg gcggccggca cgtggcaccc
    11161 acctttgccg gcgacaccct ctatgcttgg tcggagattt tgcaggtcgc cgaagttccg
    11221 ctgcggcctg acatcggcgc cctacgcacg cgtctcgttg ccgtgaagaa ccgcagctcg
    11281 gccgattttc ccctgcaaac gccccagggt gaggacgccg atggcgtcgt gctcgatctc
    11341 gatctgtggc tggtcctgcc gcgctaggcc ccgcccgctg gccgcagccc gaccctggta
    11401 taccagcctt gcgcgcattg ctttcgccgc cgcgggcgcc catgtgatgt ttgagcggat
    11461 tgccgcccca gctgccacag gctaaaagcc gatgctgcag cttcgcggga gtaaccaaag
    11521 gcatggccga caccggtgag agcaccgcac gccccttatc gccgcacctt tcggtgtatc
    11581 gaccgctgat caacatggtg atgtcgatcc tgcaccggct tacgggtgcg gcgctgtatg
    11641 ttggcacgct cttgctcgcc gcctggctga ttgcggccgc cagcggggaa gcacaattcg
    11701 cgcgcatcaa cagcctcttt gcccacccgc tcgggaggct cgtgctgttc ggctacagct
    11761 gggccttgct gcaccatatg ctcggcggcg ttcggcatct cgtttgggac accggccgcg
    11821 gcttctcaat tgcccaagtc aacgcgctct cctggctcac catcatcggc tcggtcctgc
    11881 tgaccctggc cctgtggatg gtcggtctga cgctcagagg agcgctttag gatggtcggc
    11941 taccgcacgc cgctcgcccg cgtccgcggc ctcggttcgg ccaaggatgg caccgagcac
    12001 ttctggaaac aacgcctcac ggcggtcgcc aacctgatct tgatctcgtt tgcgatttat
    12061 ctcgtcgtgc ggctggccgg tgcggattat gcgaccgtga agaagacgct ggcgcggccg
    12121 cagaatgcca tcccgctgct gctgttgatc ctgtccggcg tgatccacat gcggctcggc
    12181 atgcagaaca tcatcgaaga ctatgtccac gcggagggct acaaggtcgc ggccttgttg
    12241 ctcaatagct ttttcacggc actggtcggg ctcgtgtgcg cctatgcggt gctgaaactg
    12301 agtttcggag cctgagcgat gccgaagagc actctcgata agggcaacgg catcaccaag
    12361 cccgcgctca acggtaaggc ctatccctta accgatcacg tctacgacgt cgtcatcgtc
    12421 ggtgccggcg gcgccggcct cagggcgacg ctgggctgcg cggtcgcggg cctcaagacc
    12481 gcctgtatta gcaaggtgtt cccaacgcgc tcgcatacgg tcgccgccca gggcggggtc
    12541 gccgctgcgc tgggcaacat gggccccgat gattggcgct ggcacatgta cgacaccgtc
    12601 aagggcgccg attggctcgg cgaccaggac gccatcgaat atctgtgccg caatgccccg
    12661 gaagccgttt acgaactgga gcactatggc gtgcccttca gccgtaccga agacggccgc
    12721 atctaccagc gcccgttcgg cggtatgacg acacactacg gcgaaggccc gccggcgcag
    12781 cgcacggccg cggcggccga ccggaccggc catgccatgc tgcacacgct ttacgggcag
    12841 tccctgcgcc atgcggccga gtttttcatc gagtattttg ccatcgatct catcatggat
    12901 caggatggcg gctgccacgg cgtcgtcgcc ctcaagctcg acgacgggac gctgcatcgc
    12961 ttccgcgcca aaaagaccat cctggccacc ggtggctacg gtcgcgccta tttcacctgc
    13021 acgtcggcgc acacctgtac cggcgacggc aacgccatgg ttctacgcgc cggtctgccg
    13081 ctggaggaca tggagttcgt ccagttccac ccgaccggag tttacggggc tggcgtgcta
    13141 atcaccgagg gtgcccgcgg cgaaggcggc tacctgacca atgccagcgg cgaacgcttc
    13201 atggagcggt atgccccgca cgtgaaggac ctcgcctcgc gcgacgttgt ctcccgcgcc
    13261 atgaccatcg agatccgcga aggtcgcggc gtgggacccg acaaagacca catcttcctg
    13321 catttggatc atttggatcc caagattttg cacgaacgcc tgccgggcat cacggaaagc
    13381 gccaaggtat tcgccggcgt cgaccttcgg cgcgagccga tccccgtgct gccgaccgtg
    13441 cactacaata tggggggcat tcccaccaac tatcacggcg aaatgctgac cctgaagggc
    13501 ggcaatcccg actacgtcgt gcccggcctg atggcgctcg gcgaggccgc ctgcgtctcc
    13561 gtgcacggcg ccaatcgcct gggcagcaac tcgctcatcg atcttgtggt gttcggtcgc
    13621 gcggcgggtc tgcagtgcgc caagaccatc gagcccggcg gcacccatcc cgatcttccc
    13681 gccgacgccg gcgaactggc gcttgcgcgc ctcgatcgct ttcggcacgc gagtggcggc
    13741 acgccgacgg cggcgctgcg cctgagaatg cagcgcatca tgcagatgaa ctgttcggtg
    13801 ttccgcgatg gaccggtgct caaggaaggc ctggcgaaga tcaccgatgt ctggaagggc
    13861 gtcagcgaca ttcgtgtctc ggaccgctcg ttgatttgga actccgattt ggtggagacc
    13921 ttggaattcg acaatttgat cgcccaggct gccgtcaccg tggcggcggc cgccaaccgc
    13981 acggaaagtc gcggcggtca tgcgcgcgag gactatccgg agcgggatga cgagaactgg
    14041 atgaaacaca cactgacgtg ggcggatgat cggacgcaaa gcgtgcgcct cgactaccgc
    14101 ccagtgcacg cctacacgct gaccaacgaa gtaaattatt tcccgcccaa gcctcgggtg
    14161 tattgaggca gcgctgaaga cctaaagtcg cccggcgccg ccggggccct gggctcggag
    14221 gcaacttggc ggggacgcgg ccactcagga tcgccattgc tggcgcgggg accgcggggt
    14281 tggccgccgc cgcctttctc gcgcgcgccg gccacgacgt gcgcgtcttt gagcgcttcg
    14341 atcggccgcg gcccgtcggg gctggcctgc tgctgcagcc aaccgggctg gcttgtctcg
    14401 cccaactggg gctcgacgaa gccgcgctcg cctatggcca agccattcac agcatttacg
    14461 gggagacccg cggcgggcgg ccgattttgg acatcagcta ccacgaactg gggtcccatc
    14521 tgttcggtct cggcatccat cgcggcgcgc tgttctccgt gctgcacgcc gaagtgctgc
    14581 ggctcggcat tcccctgacg acgacgacgg aaatcgccgc cagccgcgcc acggcgggcg
    14641 gccgcatcct ggtggatcgg cacaagtatg agcacggcgt gttcgatctt gtcgtcgatg
    14701 cgacgggtgt gcgctcgccg ttgcgcgcgg acgctgcctc gcttcgtcgt gatcggcctt
    14761 acgcctatgg cgcggtctgg ggaagcctcc tgcaacccgc cgattggccg caccaggatc
    14821 gacttctgca acgctacgac ggctgtcaca gcatggtcgg acttttgccc atcggccgga
    14881 cgccggacga tgctcggcgc ctagtcgcct tgttttggag cctgcgggtc acggattttc
    14941 ccgcctggcg ggcggctggg ctcgaggcct ggaagagccg gctcgtcgag gtctgccccg
    15001 aagccaaacc cttcttggcc cagctgacat cgctcgatgc tatgacgctt gcgagttatg
    15061 ccgacattgc cttgggccgg cgccgcggct cccgtatcgt ctcgatcggc gatgccggcc
    15121 gggcgctcag cccccagttg ggacagggag ccaatctcgc cctgatcgat gcggcaacgc
    15181 tggcgcaatg ccttgccgaa acctcactgg aggcggcgct cgacgcctac cgtgcccgcc
    15241 gccggcgaca cagcaatttc tatgccttgg ccagccgctg gctcaccccc ttctttcaat
    15301 ccgagagccg ctgggctgcc cgcctgcgcg acctcgtctt ccccgtgctg ggccaggtcc
    15361 cctatttgcg gcgccagatg gcctcgactt tggcgggcat gaagaccggc gtcgtgacgc
    15421 aggtcgaccc ggggcagtgg catccgagct atgccctgcg ccggcaataa accgacccca
    15481 gacgccgcga tcgcccgcac aagcgcaaag cgcacggtgc gcggggagag cgggcgcgca
    15541 acctcgcacc aacacgaaac tgacggcgag agcgcctcag gcgctacggt agagcttcgt
    15601 catgacgaat tcgcgatgcc cgagcgcttc ggcggcggtc aggcggccat tcgccgtgcg
    15661 cacgatcatg tcgatcaagg catcgccggc cgtgttgagg gtcagctcac gccgcaggat
    15721 accggagaca tccaaatcga tgtgctcggg catggtgcgc acggtcttcg gattaccgct
    15781 gattttgatc accgggacga tggggttgcc gatgatgttg ccctggcccg tcgggaaggt
    15841 gtggacgaca tagccgcccg ccgccatcaa cgtcacgcat tcagcggcgg ccgaagaggt
    15901 atccatgtaa tagagcccgg ggccggcggc cggcgtttgg gcgggctcca gcacgtcgat
    15961 atagcggcac ttgcgcccga tcttctccag attgcccagc gccttttcct cgatggtcgt
    16021 gaggcctccg gcgatattgc ccttggtcgg ctggctttcg gacagatcgt ccaccttatg
    16081 ggcttcgatc acctccgcct ggtaggcttg gaaggtcttc atccatttgt cgccgatttt
    16141 ggcgctggcc gcgcgattgc gcgcgatgtg ctcggcaccg gttatctctg aggtctcccc
    16201 gaagcaacca taaatgcctt tcgggatcag cttgtcgtac atgttgccga cggtcggaca
    16261 ggaggcaaga ccggtggtcg tatcactctc gccgcatttg gtggagaccc acagctcgtc
    16321 gatggcgcac tcctcgcgct cgagctcgga ggcccactgc aaaaactcct tggccttatg
    16381 ggaggccatg gcgatcgtgg cgatgtcgcc atggccctcg atgccgaagc ccacaaccgg
    16441 cttgccgctc ttggcgatgc catcgacgac acgcttggtc caaccatctt cgatgccgat
    16501 caccacggcg gcggccacgt tgggattcga gccggtgccg atcaaggtgc gaaaatgtaa
    16561 atcgaggtcg gcgccgaatt gcaatcgtcc gtaggcgtgc ggtaacgcca aggtcccctt
    16621 gatgttgtta gccaccgcct cgcaagccgc gttcgacagg tcatcgagcg gcagaatgat
    16681 cacgtggttg cgtacgccaa cgcgatcatt gtcacggcgc cagccgcgga agctcaaatt
    16741 gctcttggct ctcatgcgtt tctgctgcgg cttggccggc atgggctgcc ttttcaccaa
    16801 cgctttgtct tcaagttctg aacatgcacg tgtttgcccg tggccacatc ggcaatcatc
    16861 ttgc
//
LOCUS       DASTMT010000123         7654 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_1378152, whole genome shotgun sequence.
ACCESSION   DASTMT010000123 DASTMT010000000
VERSION     DASTMT010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 7654)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7654)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7654
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1378152"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            385..1287
                     /locus_tag="VFR00_02565"
     CDS             385..1287
                     /locus_tag="VFR00_02565"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEU0158163.1"
                     /translation="MTQSLSALASLAMLGAVCLWSSATPVTKLAVAEIAVGEFVTLRL
                     ALAAAALWLIVAVTGAKAHLRGVGWRPLVMGLLEPGLVTLVVSVGLTMTSPVNGSVFW
                     SLTPLLMPLLGYLVLGERLELAVLAAAAMAFAATMLLVWGQNQHGGGSWLGDMFVAGG
                     VIASAVNALLARRTAQAGANPLVTSCWQLTAGAMLAALLAAFLPATGAHVFDASRPAL
                     LSLVYLGLVVSGGVFILSNYALRHLPVGRAGLFSCLVGPVGTAMAALLLGTELSSLDL
                     VALAMVVGAVLLPSLLAWRRAPSR"
     gene            1410..2531
                     /locus_tag="VFR00_02570"
     CDS             1410..2531
                     /locus_tag="VFR00_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006329722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0158164.1"
                     /translation="MGTGVGLRQVGYLDCAGGGQVVVVDGIAYIGHMASPHGTSIVDV
                     RDPSDMRVLATLGMPPGSHSHKVRVANGIMLVNHEINGADRRPLPADFKSGLGIYDVA
                     NPAKPREITRWTTVGKGVHRFDFDGRYAYLSATLEGFVGTVMLIMDLERPERPGEVGR
                     WWMPGQWTAGGEQPSWDKDAHRCHHPLRLGNRLYTSYWKGGFVILDIDDLAHPRLLSG
                     LNWRPPYTCPTHTALPLPFAINGRRYLVVADEDVQRGEHDVAAFAWMVDITDERRPVP
                     VGSFQVEGIEGRPSAAMTACHQPCEKVTGSEIPFAWFAQGLRIIDVGNPHALREVAHF
                     LPDVPPGTDGVSSNDLTVDERGLIYLIDRRRGLTVLERT"
     gene            2606..4402
                     /locus_tag="VFR00_02575"
     CDS             2606..4402
                     /locus_tag="VFR00_02575"
                     /inference="COORDINATES: protein motif:HMM:NF014585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HEU0158165.1"
                     /translation="MAETVDAITLELFKNAIFSIADEMALTVFRTTYSGVLKDNMDYS
                     TGFADAEGRLAAQGLTLPGHLGSVPTAMAAIMRHFGDAIAEGDVFIMNDPFDGGMHLP
                     DIFVMKPLFHAGERLAFACTVCHHCDVGGRVAGSNASDSTEIYAEGLRIAPMKLYAAG
                     KPNETILTFIEKNVRLPVQLFGDLRAQLAACHIAEKQFAELVARYGAEPTKVLLQATI
                     DHAERLTRAALGELPDGEWSFEDWIDDDGIDYGKPIRLFVTIRKRGGHMVVDWTGSNR
                     QVKGAINNTLSFTKAASYTAVRSVLPPDIPNNEGVFRAIEVICPPGTVGNAVLPAACA
                     ARGLTGFRMTDCMFGALAMMLPDKVKAAGDGGNTGISIGGYDQQRQPFVYVDFTCGAW
                     GARPWADGLDGNSHMFANMASHSVEVTEAEQPISLLAYEFVADKAGAGKYRGGVPFRR
                     DYRFNETEGVLQVRADRRDHRPFGLYGGSPGAPSENYLNPAQENRGLPGKFTMTIKQG
                     DVFRHVLAGAGGWGDPLERDPDAVLWDVRNELLSPAKAAADYGVLLDATAASVDLAAT
                     AERRAQLRQQRGWRVTPVVQREEPQPQAAAQE"
     gene            4406..6490
                     /locus_tag="VFR00_02580"
     CDS             4406..6490
                     /locus_tag="VFR00_02580"
                     /inference="COORDINATES: protein motif:HMM:NF014071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HEU0158166.1"
                     /translation="MVRYRVGVDIGGTFTDIVLLGSDGKIHTLKLSSSADDYARAIVE
                     GLGEVFEQSDVSAAAIEEIRHGTTVASNAILERKGARVGLITTEGFRDVLEIRTLRMP
                     RLYDLAWSKPEPLVPRYLRQTVGERIDHAGRVERALQAADAERAVARLLAAKVEAIAI
                     CLLNSFANPVHELMLGEIVRRMAPQMPLSISYQVLPEIKEYERTSTTVINAYVMPIVA
                     TYLGALRRRLDAASIAARLLIMQSNGGLMTSATASERPMHIIESGPAGGVVGAQALAR
                     AIDVNKIITFDMGGTTAKASIVEDGELMRAHEYAVGAGIMTGSRLLTGAGYILKVPAI
                     DLAEVGAGGGSHVWLDAGGSLKVGPQSAGASPGPVCYGLGGEVPTVTDANVILGFINP
                     KHLIGGALPLDAARAAEVFEAKIAKPLGLDLATAALGAHDIAASNMIRAIKAVSTERG
                     RDPRQCALFAFGGNGPLFACGMARSLGISQVIVPPAPGLFSSFGLLWADVEHHYSRTF
                     RRRLRGADLAAIEAAWGALAAQALAQLESEGFTGERARLRRSAALHYHGQSFDLTVPV
                     PERPFDAAMVAHLEEAFGREHERTYGHRAGAAEPVELVSIQVVGQGVRSGPAMPASVV
                     VGRREAPPGPPRRAYFGAQQGWLETPIVPRSALTKVRTGPLIVEEYDATCLVPPGASA
                     RLDAGGNIRIEL"
     gene            6509..>7654
                     /locus_tag="VFR00_02585"
     CDS             6509..>7654
                     /locus_tag="VFR00_02585"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEU0158167.1"
                     /translation="MTQPTTNSHIEQAYRARTPRSAALYARARQSIPAGLTHDSRTLL
                     PYPIYASKAAGSRKWDVDGNEYVDYFGGHGALLLGHGHASVTQAVARQLELGTHWGAA
                     HELEVAWAELVARLIGCAERVRFTASGTEASHLALRLARAFTGKRKVIRFVGHFHGWH
                     DGVAAGGMSHFHGGVPAGILPGLVEETLLLPVDDLERVARTLEAASDVAAMVLEPSGA
                     SWGQVPLPPGFLAGVRALSRQHGVVLIFDEVITGFRWSRGGAQGRYGVTPDLCILAKI
                     LAGGLPGGAVAGRADILDQLDAAAAAAAKREKIGHQGTFNANPLSAAAGVAMLSRLQH
                     EDLCGLAERTAAELRHGLTQVLIEEGVPWGIYGEASAFLIFPNPKRLA"
BASE COUNT         1142 a         2617 c         2585 g         1310 t
ORIGIN      
        1 cacgcggcga aatcgtcccc ggcgatgccg aggtccgcgc ctgggccctg atgggaattt
       61 ccgacatgat cgggcgccgc tttgcgctct gggacggcga aaccccgctg cggcgcatcg
      121 ccgaggccgc ccacgccctg atcctcaatg ggctggcgcc gcgcaagccc taatcggcgc
      181 ctggctgcac cggctcaatg gccggcacgg tcgtggctgc ggcgggcacc ccccctggcg
      241 cgcaccgagc gcgggggtgc caggcgtccg cggcaagcct cgcgcccttg cgagaccgcg
      301 ggccggtgtg agaagccttt gggcgcaaca tccctgccgc ccggcctgcc gttcccgctt
      361 ttcccaaccg cccgaagacg ccgcgtgacc caatccctct ctgcgctggc cagcctcgcc
      421 atgctcggcg cggtttgcct gtggtcctcg gcgacgccgg tgaccaagtt ggcggtcgcc
      481 gagatcgccg tcggcgaatt cgtgaccctg cgccttgcgc tcgccgccgc ggccttgtgg
      541 ctgatcgtgg cggtgacggg cgccaaggcg cacctgcgcg gcgtcggttg gcgtcccctg
      601 gtgatggggc tgttggagcc ggggttggtg acgctggtcg tctccgtcgg gctcaccatg
      661 acctcccccg tcaacggctc ggtgttctgg agcctgacgc cactgctgat gcccctgctg
      721 ggctacctgg tgcttggcga gcggctggaa ctggccgtcc tggccgccgc cgcgatggcc
      781 tttgccgcca ccatgctgtt ggtgtggggg caaaatcagc acggcggcgg cagttggctc
      841 ggcgatatgt ttgtggcggg cggcgtcatt gcctcggcgg tcaacgcgct gcttgcccgg
      901 cgtaccgcgc aggccggcgc caatcccctc gtcacctcct gttggcaatt gacggccggc
      961 gccatgctgg ccgcgctgct ggcggcgttc ttgccggcga cgggcgcgca cgtcttcgat
     1021 gccagccggc cagcgctcct ctccctcgtc tacctgggtc tcgtcgtctc tggcggggtc
     1081 ttcatcctgt cgaattatgc gctgcggcat ctgccggtgg gacgcgcggg cctcttcagc
     1141 tgtctggtgg gcccggtcgg gaccgccatg gccgcgctgc tcttgggcac cgagctcagc
     1201 agcctcgatc tggtcgcgct cgccatggtg gtgggcgccg tgctgctgcc cagcctgctg
     1261 gcctggcgcc gagccccatc gcgctgagcg ctggcccctt gccgagccgg cgctggcgtt
     1321 gagtttttgc cgcggccaat cggagcttgc gggaggctcg aagcctcccc gatactgagc
     1381 ttgggtgagg cgaaggcagg aggcgagcct tgggcacggg cgtgggtttg cggcaggtgg
     1441 gctacttgga ctgtgccggt ggcggccagg tcgttgtcgt cgatggcatc gcctatatcg
     1501 gccacatggc gagcccgcat gggacctcga tcgtggacgt gcgtgaccca agcgatatgc
     1561 gggtgcttgc cacgctgggc atgccgccgg ggtcgcactc ccacaaggtg cgcgtcgcca
     1621 acggcatcat gctcgtcaat cacgagatca acggggccga ccggcgcccg ctgccggccg
     1681 acttcaaaag cggccttggc atctatgacg tggccaaccc ggccaagccg cgcgagatca
     1741 cgcgctggac caccgtcggc aagggcgtgc accgctttga tttcgacggc cgctacgcct
     1801 acctgtccgc gacgctggaa ggtttcgtcg gcaccgtcat gctgatcatg gatttggaac
     1861 gcccggagcg gccgggggaa gtcggccgct ggtggatgcc gggacaatgg acggcgggag
     1921 gcgaacagcc gagctgggac aaggacgccc atcgctgcca tcatccgctg cgcctgggca
     1981 accggcttta caccagctac tggaagggag gcttcgtcat cctcgacatc gacgatctcg
     2041 cccacccacg cctgctttcg ggcctcaatt ggcgtccgcc ctatacctgt cccacacaca
     2101 ccgcgctgcc cctgccgttc gccatcaacg gccgtcgcta cctcgttgtc gccgacgaag
     2161 acgtgcagcg cggcgagcac gacgtggcgg ccttcgcctg gatggtcgac atcaccgatg
     2221 agcgccgacc ggtgccggtc ggcagctttc aggtcgaggg catcgaaggc cgtccttccg
     2281 ctgccatgac ggcctgccat cagccctgcg agaaggtgac cggcagcgaa atccccttcg
     2341 cctggttcgc ccagggcctg cgcatcatcg acgtgggcaa tccgcacgcc ttgcgcgagg
     2401 ttgcccactt cttgcccgat gtacccccgg gcaccgatgg cgtctcgagc aacgatctca
     2461 ccgtcgatga gcgcggcctc atctatctga tcgatcgtcg gcgtggcctc accgtcctgg
     2521 agcgcacctg aagggaccgc cgccgccggc cgttgcccgg cagcttgccc gccatagtga
     2581 gcaaacgatc ttgggagatg ctgggatggc cgaaaccgtt gatgcgatca cgctcgagct
     2641 cttcaagaac gcaatttttt ccatcgccga cgaaatggcc ttgacggtgt tccgcaccac
     2701 ctattcgggc gtgctgaaag acaacatgga ctactccacc ggctttgccg atgccgaggg
     2761 acggctcgcc gcgcaaggcc tgacgctccc cggtcatctc ggatccgtcc cgaccgcgat
     2821 ggcggccatc atgcgccact tcggcgacgc gatcgcggag ggcgacgtct ttattatgaa
     2881 cgatcccttc gacggtggca tgcatctgcc cgacattttc gtgatgaagc ccctctttca
     2941 cgccggcgag cggttggcgt ttgcctgcac ggtctgtcac cactgcgacg tcggcggccg
     3001 cgtcgccggc tccaatgcct ccgactccac ggaaatctac gccgagggac tgcgcatcgc
     3061 gccgatgaag ctttatgccg cgggtaagcc aaacgaaacc atcctgacct tcatcgagaa
     3121 gaacgtgcgc ctgcccgtgc agctgtttgg tgacctgcgg gcgcagctcg cggcctgcca
     3181 tatcgcggag aaacaattcg ccgagctggt ggcgcgctac ggtgccgagc ccaccaaagt
     3241 cctgctgcaa gcaaccatcg atcatgccga acgcctgacc agggccgcgc tcggcgagct
     3301 gcccgatggc gaatggagtt tcgaggactg gatcgacgac gatggcatcg attatggcaa
     3361 gccgatccgg ctgtttgtca ccatccgcaa gcggggtggc cacatggtcg tcgattggac
     3421 gggcagcaac cgccaagtca agggcgccat caacaatacg ctatcgttca ccaaggccgc
     3481 ttcctacacg gcggtgcgct cggtgctgcc gcccgacatt cccaacaacg agggggtttt
     3541 tcgcgccatc gaggtgatct gtccgccggg cacggttggc aacgccgtgc tgccggccgc
     3601 ctgcgcggcg cgcggcctga cgggcttccg catgaccgat tgcatgttcg gggccttggc
     3661 catgatgctg cccgacaagg tcaaggcggc cggcgacggc ggcaataccg gcatctccat
     3721 cggtggctat gatcagcagc gccagccctt cgtctacgtg gatttcacct gcggtgcttg
     3781 gggggcgcgg ccgtgggcgg acgggctcga cggcaactcc cacatgttcg ccaacatggc
     3841 gtcgcattcg gtggaggtaa ccgaggccga gcaaccgatc tcgcttctgg cctacgagtt
     3901 cgtagccgac aaggcgggtg ccggaaaata ccgcggcggc gtgccgtttc ggcgcgacta
     3961 tcgcttcaac gagacggaag gggtgctgca ggtgcgcgcc gaccggcgcg atcaccggcc
     4021 ctttggtctc tatggcggca gccccggggc cccctccgag aactatctca atccggccca
     4081 ggaaaatcgc ggcctgcccg gcaagttcac catgaccatc aagcagggcg atgtcttccg
     4141 gcacgtgctg gccggcgccg gcggttgggg cgacccgctc gaacgcgatc cggacgcggt
     4201 gttgtgggac gtacgcaacg aactcctgag cccggcgaag gccgccgccg actacggcgt
     4261 gctgctcgat gccacggccg cgagcgtcga tcttgcggca accgccgagc gccgggcgca
     4321 gttgaggcag cagcgcggct ggcgtgtcac cccggtcgtg cagcgcgagg agccgcaacc
     4381 gcaagcggcc gcgcaggagt aagccatggt tcgctatcgc gtcggcgtgg acatcggcgg
     4441 caccttcacc gatatcgtgc tgttgggttc ggatggcaaa atccacacgc tcaagctgtc
     4501 atcgagcgcc gatgattacg cccgcgccat cgtcgagggg ctcggcgagg tgttcgagca
     4561 aagcgacgtc tcggcggcgg cgatcgaaga aatccgccat ggcactacgg tggcctccaa
     4621 tgccatcctg gaacgcaagg gcgcgcgcgt cgggctcatc accaccgagg gtttccgcga
     4681 cgttctggaa attcgcaccc tgcgcatgcc gaggctttac gaccttgcct ggtcgaagcc
     4741 agaacccttg gtgccgcgct atctgcgcca aaccgtcggc gagcgcatcg accacgccgg
     4801 tcgcgtcgag cgggcgctgc aggccgccga cgccgaacgc gcggttgcgc ggctgctggc
     4861 cgcgaaggta gaggcgatcg ccatctgcct cctcaactcc ttcgccaacc cggtgcacga
     4921 gctgatgctg ggcgagatcg tcaggcgcat ggcgccgcag atgcccttgt cgatttccta
     4981 tcaggtcttg ccggaaatca aggagtacga acgcacctcg accacggtga tcaacgccta
     5041 tgtgatgccg atcgttgcca cctacctcgg cgcgctgcgc cggcggctcg atgcggcctc
     5101 cattgccgcg cgcctgctca tcatgcagtc gaacggcggt ttgatgacga gtgcgacggc
     5161 cagcgagcgg cccatgcata tcatcgagtc gggccccgcc ggcggcgtgg tcggcgcgca
     5221 ggcgctcgcg cgcgcgatcg acgtgaacaa gattattacc ttcgacatgg gcggcaccac
     5281 ggccaaagcc tccatcgtcg aggacggcga gctgatgcgc gcacacgaat atgcggtcgg
     5341 tgcgggcatt atgaccggtt cacgcctgct gacgggtgcg ggttatatcc tcaaggtccc
     5401 ggcgatcgat ctggccgagg tcggcgccgg cggtggatcg cacgtctggc tcgatgccgg
     5461 cggtagcctc aaggtgggac cccagagcgc cggtgcgtcg ccggggcccg tctgctatgg
     5521 cctcggcggc gaggtgccga cggtgacgga tgccaacgtg atcctgggct tcatcaaccc
     5581 caagcatctg atcggcggcg ccctgccgct cgatgccgcc agggcggcag aggtgttcga
     5641 ggcaaaaatt gccaagcccc tgggcctcga tctggcgacg gcggcactgg gcgcccacga
     5701 catcgccgcc tccaacatga tccgcgccat caaggcggtt tcgaccgagc gcggccgcga
     5761 cccgcggcag tgtgcgctgt tcgccttcgg cggcaacgga ccgctgttcg cctgcggcat
     5821 ggcgcgctcg ctcggtatct cccaggtgat cgtgccgccc gcacccgggc tgttttcctc
     5881 ctttggcctg ctctgggcgg acgtagagca ccactattcg cgcaccttcc ggcggcggct
     5941 gcgcggcgcc gatctcgccg ccatcgaggc ggcctggggt gcgctcgcag cccaagcgct
     6001 ggcgcagctc gaaagcgagg gcttcacggg cgagcgggcg cgcctgcgcc ggtccgcggc
     6061 gctgcactac cacggacaga gcttcgatct gacggtgccc gttcccgagc ggccgttcga
     6121 tgccgccatg gtcgcgcatc tggaggaggc ctttgggcgc gagcacgagc gcacctatgg
     6181 tcaccgcgcc ggcgccgccg agcccgtcga gctggtgtcg atccaggtgg tgggccaggg
     6241 cgtgcgctcg gggccagcca tgccggccag tgtggtcgtg ggccgccgcg aagcgccgcc
     6301 cggcccgccg cgccgggcct atttcggggc gcagcaaggc tggctggaga cgccaattgt
     6361 gccgcgctcc gccctgacca aggtgcgcac gggacccctg atcgtggagg agtacgatgc
     6421 gacctgtctc gtgccgcccg gggcgagcgc caggcttgat gccggcggca acatcagaat
     6481 agagctttag ggtaagcgcc gaggagccat gacgcagccg acgacgaatt ctcacatcga
     6541 gcaggcctac agggcccgaa ccccgcgctc tgccgcgctc tatgcccggg cgcgccaatc
     6601 gattcccgcg ggtctcacgc acgacagccg caccctgcta ccttacccga tctacgccag
     6661 caaggccgcc ggcagccgca aatgggatgt cgacggcaac gagtacgtcg actacttcgg
     6721 cgggcatggc gcgctgttgc tggggcatgg ccacgccagc gtgacgcagg cggtggcgcg
     6781 ccaactcgag ctcggcacgc actggggcgc agcccatgaa ttggaggtcg cctgggccga
     6841 gctggtcgcg cgcctgatcg gctgtgccga gcgcgtgcgc ttcaccgcct cgggcacgga
     6901 ggcctcgcat ctggccctgc gcctggcgcg cgccttcacc ggcaagcgca aggtcattcg
     6961 cttcgttggg cattttcacg gctggcacga tggggtcgcg gccggcggca tgtcgcactt
     7021 ccacggcggg gtacccgccg gcattcttcc cggcctggtc gaggaaaccc tgctgctgcc
     7081 ggtcgacgat ctggaacgcg tcgcgcggac gctggaggcg gcaagcgacg ttgccgccat
     7141 ggtgctcgag ccctcggggg cgtcgtgggg ccaggtgcca ttgccgccgg gctttctggc
     7201 cggcgtgcgc gcgctctccc gccagcacgg cgtcgtcttg attttcgatg aggtcatcac
     7261 cggctttcgc tggtcgcgcg gcggcgcgca agggcgctat ggcgtcacgc cggatctgtg
     7321 catcttggcg aagatcctgg ccggcggctt gccgggtggc gcggtcgccg gccgcgccga
     7381 cattctcgac caactcgacg cggccgccgc ggcggccgcc aagcgcgaga agatcgggca
     7441 tcagggcacc ttcaacgcca atccgctcag cgccgccgcc ggggtggcga tgttgagccg
     7501 tttgcagcac gaggacctct gtgggctggc cgaacgcacc gccgccgagc tgcgccacgg
     7561 cctcacccaa gtcctgatcg aggagggcgt cccctggggg atttatggcg aagcctccgc
     7621 gttcctgatc tttcccaatc ccaagcgcct ggcg
//
LOCUS       DASTMT010000124         2611 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_3346542, whole genome shotgun sequence.
ACCESSION   DASTMT010000124 DASTMT010000000
VERSION     DASTMT010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2611
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3346542"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..375
                     /locus_tag="VFR00_02590"
     CDS             <1..375
                     /locus_tag="VFR00_02590"
                     /inference="COORDINATES: protein motif:HMM:NF024934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU0158168.1"
                     /translation="MPTFLKLVQAHVLAFLEQVMVSAACNGAHNVRQRLARWLLMMRD
                     RHDGDELPLTQDLLAEMLGVHRPSITNAAQGLQRDGLIECGRRRVTILDRDGLIGASC
                     ECYRLVRTRIAYHLPKTFPEVL"
     gene            471..728
                     /locus_tag="VFR00_02595"
     CDS             471..728
                     /locus_tag="VFR00_02595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0158169.1"
                     /translation="MRARQIVESGSFGPETLKVAFQAFDEAWQSVASYFGNDPVTIEA
                     ARIKLANAILAVCHEESRDFRALKAAALATLARHYRLDLKV"
     gene            895..2208
                     /locus_tag="VFR00_02600"
     CDS             895..2208
                     /locus_tag="VFR00_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002715222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0158170.1"
                     /translation="MTEAVIGRLPEDEHRIQLRRAVVASTIGTAIEWYDFFLYSTVTG
                     LVFAKLFFPNSAPLVGTLEAFAVYAVGFVARPIGAAIFGHYGDRIGRKATLIATLMLM
                     GIATFLVAFVPTYESIGIWGAVILTVLRFVQGVGVGGEWGGSVLLSMEWSRTHHQRGF
                     IASWPQFGVPCGLFLANLAVLAFSQMSGEDFLAWGWRLPFALSIVLVAVGLYIRLGIL
                     ETPTFTRLQEAERLERAPVPAVLRGHGKEILLSAFARMAEQAPFYIFTAFVFAYAVNT
                     LKVPRDFILTSVLAAAVLSFISIPLFGHISDRIGRKKTYMIGAALTGVFGFVYFAMLD
                     TGYYPIIFLAIVLSLIPHDIMYGPQAALIAELFPGRLRYSGASLGYQLASVIAGGPAP
                     LIATWLFATYGTPYAIAVYILACAIVSLIATAMMRDYTGKALSDH"
     gene            complement(2262..>2611)
                     /locus_tag="VFR00_02605"
     CDS             complement(2262..>2611)
                     /locus_tag="VFR00_02605"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HEU0158171.1"
                     /translation="TRQAVNNLEEILSVQGVSGVYVGPSDLSLSLGKPPTLDPSDKEV
                     LAAIDTIVKTTRKKGLIAGVHTDGPATALRRFEAGFQMCTILNDVRLLAVAAANAINE
                     VRGKAPAAKAKTY"
BASE COUNT          382 a          890 c          801 g          538 t
ORIGIN      
        1 atgcccacct tcctcaaact cgtgcaggcc cacgtgctgg cctttctcga gcaggtgatg
       61 gtatcggccg cctgcaatgg cgcccataat gtgcgccagc gcctggcgcg ctggctgttg
      121 atgatgcgcg accgccacga cggcgacgag ctaccgttaa cgcaggatct gctggccgag
      181 atgctgggcg tgcaccgccc cagcatcacc aatgccgccc aaggtctgca gcgcgacggc
      241 ctgatcgagt gcggccggcg gcgcgtcact attcttgatc gcgacggcct gatcggcgcc
      301 tcctgcgagt gctaccgact ggtgcgcacg cggatcgctt atcacctgcc aaaaaccttt
      361 cccgaggtgc tttaggtcat ctaacttacc gacagctccg ggcctccaga ccatggtcca
      421 ctccatgtct ggccgcctgt gcgggggcgg attttcgccc tgaggacctc atgagagcac
      481 gtcagatcgt cgaaagcggg tcctttggac ccgagaccct caaggttgcc tttcaagcat
      541 tcgatgaggc gtggcaaagc gttgccagct attttggcaa cgatcccgtc accatcgagg
      601 ccgcgcgcat caagctcgcc aacgccatcc ttgccgtttg ccacgaggag agccgcgact
      661 tccgtgccct gaaggcggcg gcgcttgcca ccctggcgcg ccattaccgg ctcgacctca
      721 aggtttaagc cgccggccgc cgcagcatcg gcccttgctg ccccgcggcc aggtcggccg
      781 cccccagccg ccgcaaccgg cgctgcgacc cagggggcgc gccgcttgga accttggcgc
      841 cggccgccac gttgtacacg gtcggcagtc gggcgggaca gcatggagtg ctgaatgacc
      901 gaagctgtca ttggccgtct ccccgaagac gaacaccgca ttcagctgcg ccgggccgtc
      961 gtcgccagta ccatcggcac ggcgatcgag tggtatgatt tcttccttta cagcaccgtg
     1021 accggcttgg tgttcgccaa gctgtttttc cccaactccg cccccctggt cggaaccctc
     1081 gaggcctttg ccgtatacgc cgtcggcttt gtggcgcgtc ccataggcgc ggcgatcttc
     1141 ggccactatg gcgaccgcat cggccgcaag gcgacgctga ttgccacctt gatgctgatg
     1201 gggattgcca ccttcctggt cgccttcgtg cccacctacg agagcatcgg catctggggt
     1261 gccgtgatcc tgaccgtctt gcgtttcgtg caaggcgtcg gcgtcggtgg cgagtggggc
     1321 ggctcggtgc tgttgtcgat ggaatggtcg cgcacccacc accagcgcgg cttcattgcc
     1381 tcctggcctc agttcggcgt gccctgcgga ttgtttcttg ccaatttggc cgtgctcgcc
     1441 ttcagccaga tgtctggcga agactttctg gcttggggtt ggcggctgcc gtttgcgctc
     1501 agcatcgtcc tggtggcggt cggtctttat atccgccttg gcattctcga gacccccacc
     1561 ttcacgcgcc tgcaggaggc cgagcgcctg gaacgcgcgc cggttccagc cgtgctgcgc
     1621 ggccacggca aagagatcct gttgagtgcc ttcgcccgca tggccgagca ggcgccgttc
     1681 tatattttca ccgccttcgt ctttgcctac gccgtgaaca ccctcaaggt gccgcgcgat
     1741 ttcattctga cctcggtgct cgccgccgcg gttctctcct tcatcagcat tcccctgttc
     1801 gggcatatct ccgaccgcat cggccgcaaa aagacctaca tgatcggggc cgcgctcacg
     1861 ggcgtgttcg ggttcgtcta ctttgccatg ctcgacacgg gctactatcc catcatcttc
     1921 ctcgccatcg tgctgtccct gatcccgcac gacatcatgt atgggccgca ggcggccttg
     1981 atcgccgagc tctttcccgg gcggctgcgc tatagcggtg cctcgcttgg ctaccagttg
     2041 gcatccgtca tcgccggcgg ccctgcgccg ttgatcgcca cctggctgtt tgccacctac
     2101 ggcacgccct acgccattgc cgtctacatt ctggcctgcg ccatcgtgag cctcatcgcc
     2161 acggcgatga tgcgcgacta caccggtaag gcgctgagcg accactagcc ggcccccggg
     2221 ccaagcggcc cgggggcctc aaccgacgcc gtcggtcggc tctagtaggt tttggccttg
     2281 gcagcaggcg ccttgccgcg cacctcattg atggcattgg cagcggcgac cgccagcagg
     2341 cgcacgtcgt tgaggatggt gcacatttgg aagcccgcct cgaaacggcg cagcgcggtg
     2401 gcggggccgt cggtgtgcac gccggcgatc aatcccttct tgcgcgtggt cttgacgatg
     2461 gtgtcgatgg cggcgagcac ctccttgtcg ctcgggtcga gcgtcggcgg cttgccgagg
     2521 ctgagggaga gatcggaggg gccgacgtag accccgctca cgccttgcac cgacagaatt
     2581 tcctccaggt tgttgacggc ttggcgggtc t
//
LOCUS       DASTMT010000125         2864 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_2691272, whole genome shotgun sequence.
ACCESSION   DASTMT010000125 DASTMT010000000
VERSION     DASTMT010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2864)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2864)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2864
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2691272"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            318..1112
                     /gene="trpC"
                     /locus_tag="VFR00_02610"
     CDS             318..1112
                     /gene="trpC"
                     /locus_tag="VFR00_02610"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012112105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HEU0158172.1"
                     /translation="MTDILTSIIAYKREEIAAAQAARPLKEVEAAAHAAPPVRRFAEA
                     LETAVNAGRFGLIAEIKKASPSKGLIRSDFDPPSLATAYQAGGATCLSVLTDTPSFQG
                     APEHLSQARAAAMLPVLRKDFMLDRYQVAEARALGADCILIIMAAVDDVIAGTLCAYA
                     RAWNMDAIAEVHNRGELDRALRLDCRLIGINNRDLKTFETTLETTERLAPGIPDNRIV
                     VAESGITKPADLARLAKVGVHAFLVGERLMREPDVTAATRTLLAQP"
     gene            1174..1665
                     /gene="moaC"
                     /locus_tag="VFR00_02615"
     CDS             1174..1665
                     /gene="moaC"
                     /locus_tag="VFR00_02615"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HEU0158173.1"
                     /translation="MTKLSHLNAKGEARMVDVSGKSVTQRAARAEGFVAMRPASLALL
                     EAGTAKKGDVLATARLAGIMAAKKTHELIPLCHPLAITSAGVDFEVSREPCGVRVTAE
                     VKVGGQTGVEMEALTAVSIACLTIYDMLKAADKAMTVSGIRLLEKMGGRSGHFRAERR
                     SER"
     gene            1669..>2864
                     /gene="glp"
                     /locus_tag="VFR00_02620"
     CDS             1669..>2864
                     /gene="glp"
                     /locus_tag="VFR00_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004123201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HEU0158174.1"
                     /translation="MRPLSVAEALQRILDGVAPTPAETVPVEAAHGRVLAEALIARLT
                     QPPFDASAMDGYAVKSVDLGTLPSTLDVIGTAAAGHPFAGTLGPGQAVRIFTGAPVPS
                     GSDAIVIQEEAERVGDRVKILNARIDRTHIRPRGLDFEVGAALLGVGRRLGPREVSLA
                     AAMGYGRLGVRRRPRLALLSTGDELVAPGQLPGEAQIVSSNHLGVAAMAQAAGAEVEF
                     LGIARDLPESLNAHFAKAADADVLVTIGGASVGDHDLVAPVLTARGMTLAFWKIAMRP
                     GKPLMFGRLRQTRILGLPGNPVSALVCARLFLVPLLRALLGVPNAESSPLKARTAVAL
                     EANGVREHYMRATHRRGEDGLALVTPVRSQDSSLLSPLAEADCLLVRDAGAPEVPVGG
                     LVSILPL"
BASE COUNT          459 a          969 c          957 g          479 t
ORIGIN      
        1 ctgcctcgat cggttcgtgc cctcgtactg gcgacgttag cgggtctcaa agtaggcgac
       61 aacgcggcat gcgctcgcgg gccaccccca gtcctgtggc gctgatcctg atgccgagag
      121 cctccgagac tgaagcccgc cgtgacgatt tttgctctac gtcctcgtct cgagccggcg
      181 tggcggcaag ctggaggcgg caagccggca cgatggcctg gcaatcaagc tgggatcgat
      241 cccgccggtt gcgggttttg tgtgatcgca ggtttgagac agtagccgcc cgcagggccc
      301 cagcgtatgt ttcgcccatg accgacatcc taaccagcat catcgcctac aaacgcgagg
      361 aaattgccgc cgcccaggcg gcgcgtcccc tgaaagaggt ggaggcggcc gcgcacgccg
      421 ccccgccggt gcgccgcttt gcggaagctc tggaaacggc cgtcaatgcc ggacggttcg
      481 gcctgatcgc cgaaatcaag aaggcaagcc cctccaaggg cctgatccgc tccgattttg
      541 acccgcccag ccttgccacg gcttaccaag cgggcggcgc cacctgtctt tccgtcctca
      601 ccgacacgcc ctccttccag ggcgcgcccg agcatctgag ccaagctcgc gcagccgcga
      661 tgctgccggt gctgcgcaag gattttatgc tcgaccgcta ccaggtggct gaggcacgcg
      721 cgctcggcgc cgactgtatc ttgatcatca tggccgcggt tgacgatgtc atcgccggga
      781 cgctttgcgc ctatgccagg gcgtggaaca tggatgccat cgccgaagtg cacaaccgtg
      841 gcgagctcga ccgcgccttg cgcttggatt gtcgcctgat cggcatcaac aaccgcgacc
      901 tgaagacctt cgagacgacg ctggagacca ccgagcggct agcgccgggc attcccgata
      961 accgcatcgt cgtggccgag agcggcatca ccaagcctgc ggatctggcg cggctcgcca
     1021 aagtgggcgt gcacgcattc ctggtgggcg aacgcctgat gcgtgagccg gacgtgaccg
     1081 ccgccacgcg cacgctgctg gctcagccat gagccgcagc gctgctccac gccaatgcac
     1141 tggcacggcg cacacccagg ggagaacgcc agtatgacga agctctccca cctcaacgcc
     1201 aagggtgagg cgcgcatggt cgacgtctcg ggcaagagcg tgacgcagcg cgcggcgcga
     1261 gcggaaggct tcgtggccat gcgcccggcc agcctggcgt tgttggaagc gggcacggcc
     1321 aagaagggcg acgttcttgc cacggcccgc ctcgctggaa tcatggcggc gaaaaagacc
     1381 catgagctca ttccgctctg ccaccccttg gccatcacca gcgcgggcgt cgactttgag
     1441 gtgagccgcg agccctgcgg cgtgcgcgtc accgccgagg tcaaagtcgg cggccagacg
     1501 ggcgtggaga tggaggccct gacggccgtc agcatcgcct gcctgacgat ctatgacatg
     1561 ctgaaggcag ccgataaagc catgaccgtg tctggcatca ggctgttgga aaagatgggg
     1621 ggacgctcgg ggcatttccg tgccgagcgc agaagcgagc gttgagccat gcgaccctta
     1681 tccgttgccg aggccctgca gaggatcctc gatggggttg ccccgacacc agccgagacg
     1741 gtgccggtgg aagcagcgca cgggcgggtg ctcgccgagg cgttgatcgc gcgcctgacg
     1801 cagccgccgt ttgatgcctc ggcaatggac ggctatgcgg tcaagagcgt ggacctcggc
     1861 acgctgccga gcacgctcga cgtcatcggc accgccgccg ccggccaccc cttcgccgga
     1921 acgctagggc caggccaggc ggtccggatc ttcaccggtg cgcccgttcc ctcgggcagc
     1981 gatgccatcg tcatccaaga ggaggccgag cgcgttggcg acagggtaaa gatcctgaac
     2041 gcccgcatcg accgcacaca catccgcccg cgcgggcttg acttcgaggt gggcgcggca
     2101 ttgctcggcg tcggccgtcg gctcgggccc cgggaagtct cattggccgc ggccatgggt
     2161 tatggacggc tcggcgttag gcggcgcccc cgactggcgc tgctctcgac gggcgacgag
     2221 ctggtggccc ctggtcagct cccgggcgag gcgcagatcg tttcctcaaa tcatctgggc
     2281 gtcgccgcca tggcgcaagc ggcgggcgcc gaggtcgaat tcctgggtat cgcccgcgac
     2341 ctgccggaga gcctcaacgc ccatttcgcc aaagcggccg atgcggatgt gttggtcacc
     2401 attggcggcg cctcggttgg cgatcacgac ctggtggcac cggtactgac cgcgcggggc
     2461 atgactttgg cgttctggaa aatcgcgatg cgaccgggca agccgttgat gtttggtcgg
     2521 ctgcgccaga cgcggatctt agggctcccc ggcaaccccg tctccgcgct cgtctgcgct
     2581 cgtttgtttc tggtgcctct gctgcgggcc ttgctcggcg tgcccaacgc ggagagctcc
     2641 ccgctcaagg cgcgcacggc ggtcgccctc gaagccaacg gcgtgcgcga gcactatatg
     2701 cgcgctaccc accgcagggg cgaggatggc cttgcgcttg tgacgcccgt acgctcccag
     2761 gacagctcgc tcctttcgcc gctcgccgag gccgactgct tgctggttcg ggacgcgggg
     2821 gcgcctgagg ttcccgtcgg tggcctcgtc tccattctgc ccct
//
LOCUS       DASTMT010000126        21409 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_5941225, whole genome shotgun sequence.
ACCESSION   DASTMT010000126 DASTMT010000000
VERSION     DASTMT010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 21409)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21409)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21409
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5941225"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..680
                     /locus_tag="VFR00_02625"
     CDS             <1..680
                     /locus_tag="VFR00_02625"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEU0158175.1"
                     /translation="SAFNAILDGLERRSDWSVLHLRSALKVFSAGADLGEISGHFARA
                     DKMAAAADLTRPFQALFQRIADLPQVSLAEIGGAAMGGGYELALGCDLRIAANEAKLG
                     LPEVGLGLLPGGGGTQRLTWIAGPAVAARIILAGDSVDGATARELGMLQWAVPGETLA
                     AEAKRLAARLAGLPPLALAAAKVCIRLARDPERNGFAAETEWTGRLLATEETQRRVGD
                     FLAGVRR"
     gene            806..1549
                     /locus_tag="VFR00_02630"
     CDS             806..1549
                     /locus_tag="VFR00_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009028309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU0158176.1"
                     /translation="MLLKFKTVVLTGGASGIGRATVLMLAREGARVLLCDVNAAGAAE
                     TIKQAPEGAVDYVALDLADGASVDACAKTALERAGRKIDVFINAAGWDRTQPFVNADQ
                     AFIDKVLGINLAGPLRLTRALFPAMIEAGGGKIVFVASDAGRVGSLGETPYSAAKGGL
                     IGFTKSLAREGARHKITVNCVCPGPTDTPLFHANPERMREALLRAIPFRRLATPDDIA
                     GAILYFAGPSSDYVTGQILSVSGGLTMAG"
     gene            1617..3272
                     /locus_tag="VFR00_02635"
     CDS             1617..3272
                     /locus_tag="VFR00_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013503797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEU0158177.1"
                     /translation="MEFDIWLPAHRIAAAEKLWPGRLPINDLDAAVALGPERTAFVGW
                     NSRLGRQIRLSYGELGERVDKIAQGLAELGIGHGDVVAFQLPNWWEFIALVYACNRIG
                     AVANPLMPIFRQRELRFMLGFGEAKVAIVPATWRGFDHLAMLRQIRADLPRLGHVFAV
                     GGSGEASFEAAFLERPPLTRIEQARLAERQRTPNDVVELIYTSGTSGEPKAVMHTANT
                     VLAAAQGFVADIPVGRADVVFMGSPYAHQTGFLYGILMPIMLAGKTVALDQWSAAAAA
                     PLIESEAASFSMGSTPFLSDLVNLPQMLRSKVSTTLRTWVCAGAPIPRLLVERARNEM
                     ALDVLSCWGMTENAGLTITRKGDPQEKVIGTDGRALPGSEVRVVDSAKRPVAPDTVGH
                     LQARGITHFVGYLKRPQLNGIDDDGWFDTGDLARMDGDGYIRIVGRSKDVIIRGGENI
                     PVAEVENLIYRHPQVAECAIVAMPDPRLGERACAFVMAKADTLITLQDLTRFLDGHGM
                     AKQYWPERLELVAEMPRTPSGKIQKFKLRERAAELARGPLAAN"
     gene            complement(3273..4091)
                     /locus_tag="VFR00_02640"
     CDS             complement(3273..4091)
                     /locus_tag="VFR00_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008833928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU0158178.1"
                     /translation="MTIEDPRGRVDYEECGRGPTVVFAPGSCSTGAAWRPIISHCEAQ
                     FRCITTSLPGYGGTAERRSNADPGIAHVAAALEAVIRRAGSPVHLVGHSFGGLAALAV
                     ALRQYVPLVSLTIVEAPAAQLLEAAGEHAHYRAFRGMTQAYFTAFAAGDPEAIATMID
                     FYAGVGTFAGWPPRVRAYAVETTAVNVLDWASAYGYQLAPAMLATLQLPVLVLRGSAS
                     HPAVKRANELISQGLKHSAMATVDGAAHFMITTHAAVLAALIQDHIGRAVAKQT"
     gene            4289..5446
                     /locus_tag="VFR00_02645"
     CDS             4289..5446
                     /locus_tag="VFR00_02645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-independent methionine synthase II
                     family protein"
                     /protein_id="HEU0158179.1"
                     /translation="MAQILTTHAGSLPRPPLLTELFTRRSQGDSVDEALLATAGREAV
                     AWVVEKQRQAGIDIISNGEQQRESFVLYLRRRLSGIGGRGERAPFADIESYPKFKAER
                     ARLFAARPAVSNVAFLPKCIGPIGYTGHAELENECATFKAAVDGLGRSQAAPFLTAAS
                     PGILASIVKNEHYATFDAYLDAIAAALRIEYEGIVAAGFQLQIDAPDLALERHASFAA
                     RPLQDFQHFVERVIGAINAALVNVPADKVRLHVCWGNYEGPHDHDVPLADILPVLLQA
                     NVGTLYLPFANPRHAHEYKVLQQQPLRSNQVLVAGVIDTVTNYVEHPEVVADRLERVA
                     FALGDPQRLLAGTDCGFDTSAGMGRVAEDVVWAKLSALADGARRASQRLFG"
     gene            complement(5536..6018)
                     /locus_tag="VFR00_02650"
     CDS             complement(5536..6018)
                     /locus_tag="VFR00_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008833927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEU0158180.1"
                     /translation="MSQRSIAAAWLLLLSLGSAPLAGPALADQAHHTVVPGDAVKWGP
                     APASLPSGAQAAILLGNPAQEGPFVIRLKFPDGFAVPPHRHSKDEFLTIIAGKFAVAS
                     GEKLDRTAAKPLPAQSFVHLPAGMAHYAWADGEAIVQINGFGPFDVKYIDPKDDPRNK
                     "
     gene            complement(6194..6952)
                     /locus_tag="VFR00_02655"
     CDS             complement(6194..6952)
                     /locus_tag="VFR00_02655"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013945962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEU0158181.1"
                     /translation="MIFKADSSLVRELHPAANFEARRGGMRPSILLLHYTGVATAAKA
                     IDWLARPESRVSCHYVVDECGCVTQMVAEAARAWHAGEACWAGETDINSASIGIELHN
                     PGHEFGYPDFPAAQLDAVEALARDILARHRIRPERVLAHSDVAPRRKIDPGEKFPWSR
                     LAAAGVGHWVDPAPLIAAEPGIGVGAAGPQFAELQLLLREYGYGIEPTGVVDGPTECV
                     IRAFQRHFRPARVDGKIDHSTIDTLARLHAALRP"
     gene            complement(6949..7647)
                     /locus_tag="VFR00_02660"
     CDS             complement(6949..7647)
                     /locus_tag="VFR00_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003607339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HEU0158182.1"
                     /translation="MTVWQKIAGFATVLGDAGGSLLHEVASVFGFDRSGAPEKSVAFT
                     IGVIALSAKMAKADGVVSEREVDAFKRVFQFSAEEAANVARVFDLAKQDTAGYEAYAD
                     QIAALLGDDRRLLQHVLEGLLYVAAADGALHPKEDQFLKAVAQRFGFADNEYRYFRAR
                     FVRDRDNPYDVLKLPPSASNTEIRAQYRKLVTDNHPDKLIGRGVPAEFVDLATRKLAA
                     INAAYDSIAKERGL"
     gene            7746..8822
                     /locus_tag="VFR00_02665"
     CDS             7746..8822
                     /locus_tag="VFR00_02665"
                     /inference="COORDINATES: protein motif:HMM:NF004127.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEU0158183.1"
                     /translation="MSETPKDEVLIRREGRAGRITMNRPQALNALTHAMVGPIFEALK
                     AWCADPGVELVVLDGSGERALCAGGDVRALYDSRSRGSGAARAFWRDEYRLNALIGRY
                     AKPYVAIQDGIVMGGGIGLSAHARHRIVTERSRLAMPETGIGLIPDVGGSWLLAHAPG
                     EAGVYLGLTGAEMDAADAIHARFADHFVPSSALGELKARLLDPRGGEPGELLKRLAAP
                     VARSRLAERGPAIDHIFAGASVEAICDRLAKSDLDWSQHTCAALAQKSPKALKLTCAA
                     IRQARTLPSLEAALDVEYRLAVRLFEDGEFPEGVRALIVDKDRKPAWSPSTLQQVTDA
                     LLQRYLAPLPAAEELGLAPARTQN"
     gene            complement(8850..9734)
                     /locus_tag="VFR00_02670"
     CDS             complement(8850..9734)
                     /locus_tag="VFR00_02670"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007599867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HEU0158184.1"
                     /translation="MPVAIRQLHPVFVGEVSGIDISRPVSPADAAAIEAGMDRYAVLV
                     FPGQAVSDEQQMVFTRNFGAIENARGGNVTKEEDKRLATGMNDVSNLGKDGRPLARDS
                     RQRLFNLGNLLWHSDSSFRPIPAKYSLLSARVVNPKGGDTEFADMRAAYDALDADSKA
                     EIEDLVCEHSLMYSRGSLGFVDYTEEEKQLFKPVRQRLVRTHPVTGRKSLYLSSHAGG
                     IIGKPTPEARILLHDLKEHATQPRFVYAHKWKVNDLVMWDNRQTMHRGRRYDESQPRD
                     VRRTTVAGDAPTIAQAAE"
     gene            10464..11633
                     /locus_tag="VFR00_02675"
     CDS             10464..11633
                     /locus_tag="VFR00_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008960731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HEU0158185.1"
                     /translation="MFRFAITLGYLACLAMLATPATAQLLWPGTAEVAQATTPRRAQP
                     IPNKPVSEEEAAKAARINNWTVGVAGGLLEGTFVRYAADLGKALDDADNLRVLPIVSY
                     GAVGNVSDLIYLKGVDIAITYSDVLDHFRNVERIPGIERRVNYIIPMFQGEVHVYVRE
                     EIKSFQDLVGKKVNFNTVGSAANYTGGIVFDRLGIKVERLFQNNSVALEQMRKGEIAG
                     IVHVVGKPNDLFAKFKPEPGFHFLPVEFGAKFEDYYVPAELTAADYPNLIAQGETVQT
                     ISVPALLAVYNWNKDTDRYRRCVRFVEYLFDRFEKLRDKPYQPGWKDMNLAGTIPGWT
                     RFPPAEALVAKAAAQASAEANLSRAQATRATADNKAEQERLFKQFLEWSKQQKRR"
     gene            11860..12657
                     /locus_tag="VFR00_02680"
     CDS             11860..12657
                     /locus_tag="VFR00_02680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0158186.1"
                     /translation="MRVRSRLAGIALLCGALLMVAPSGRPGPHPATAQQGQPVSLDVA
                     AVTNAAPASRVRLPIEVRPRDALAKNSFIRIRGLPATAALSEGYAIAPGSWAVPLNGL
                     ASLMVILPVGLEGRSEVVVALVNVEGSILAEATTTLVVARAATQPPPPAPVAAAPPNS
                     TTRALSAPQLAPADRERALKLHARGQEQLGRGNIYAARQFFERAAEAGLAESALALAA
                     TYDPTELARLSVLGLKAEPAEARKWYEKARELGASEADARLRRLPAR"
     gene            12737..14329
                     /locus_tag="VFR00_02685"
     CDS             12737..14329
                     /locus_tag="VFR00_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019199090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HEU0158187.1"
                     /translation="MHTLARALLAEIEAFLAQSGLTPTKFGLAAVNDGHLVANLRKGN
                     SVTLKTADRVRAFMRERARGGAPANTPAPPALLHAAARSLTPGPAAWPFAGPDGRRRI
                     VLIIGGGIAAYKCLELIRRLRDQHVEVRAIMTRAAQAFITPLSVGALTGNRVFTELFD
                     LNEEQEIGHIRLAREADLIVVAPATADLIAKMAGGHADDLASCVLLATDRPLLLAPAM
                     NPRMWLNPATRRNVALLEADGIGFVGPGVGEMAEAGEAGPGRLAEVPDLLAAIIARLG
                     NVASPGEAQGNDRRGGDSSQPSFSPTSGATGPLAGRHVIVTSGPTHEPIDPVRYIANR
                     SSGKQGTAIAAQARLLGARVTLVSGPSQLAAPAGVDLVRVETAQEMLAAVQQALPCDI
                     AIFAAAVADWRVAETFGEKLKKGAAATAPRLELVENPDILKTIAATAPGRRPPLVIGF
                     AAETTQVVDYAKKKRKAKGADWILANDVSPKTGIMGGDRNTVHLVTATDVEDWPDLPK
                     AEVARRLVARAAQWLAEHTALT"
     gene            14326..14865
                     /gene="dut"
                     /locus_tag="VFR00_02690"
     CDS             14326..14865
                     /gene="dut"
                     /locus_tag="VFR00_02690"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEU0158188.1"
                     /translation="MSPKSTRQRSLVGVVRLAHARGLDLPRYQSDGAAGMDLIAAIAE
                     RRPLRLAPGARALIPTGLVLELPAGFEAQVRPRSGLALKHGLTVLNSPGTIDSDYRGE
                     VKVLLANLGAATCAIKRGERIAQLVFQRVERVGLREIGEASPTPRGAGGFGSTGRKHR
                     GAEAEPRAQRRQRAKTSAR"
     gene            15020..16084
                     /locus_tag="VFR00_02695"
     CDS             15020..16084
                     /locus_tag="VFR00_02695"
                     /inference="COORDINATES: protein motif:HMM:NF042637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6569 family protein"
                     /protein_id="HEU0158189.1"
                     /translation="MRFWSVAPVAAATFAAIGAAGGPLLAEQAPRLSGPHAYENLAIY
                     FVHGTSAAGTVPLTLEEGVAKGRVQVIETGRVNELMIENTGTEPVFVQAGDIVKGGKQ
                     DRVLTVSLLLPANSGRLPIQSFCVEQGRWTARGKEDANKFSSATEAMPSRAALLAMAA
                     PAPAKESGYRAAGERPDGGDVAGKQKKVWDSVAATQSKLADGVGASVNSPASGTSLQL
                     SLENEKLKGARAAYLAALKGKGEAEADILGYVVAINGKVSSANLYPSNAMFRKMWNKQ
                     LAAVVTEAIGEKKAAGDATPPPAPAQAAEFLAEAERGKPSARADAIGTRQETRDGDKT
                     LFNEAKSNAGAWLHRSYLAK"
     gene            complement(16158..17009)
                     /locus_tag="VFR00_02700"
     CDS             complement(16158..17009)
                     /locus_tag="VFR00_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036361 - racemase activity, acting on
                     amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HEU0158190.1"
                     /translation="MIGVFDSGHGGLTIVRELARRFPDLAYVYLGDHAHVPYGERASD
                     DIVALTRQGVERLFARGCRLVLLGCNTATCVAARALQRGWLPVSGWQGHNVLGIVAPT
                     VEAATQTPWAVATPQYPQKYNTDVVAVFGTTRTIASHAYPEEIHKRCPRVTVIQQACA
                     GLAGAIEAGAAEAALEGLVADAVAGLLAQTAGAPPHRAILGCTHYPLIEHLFRRHLPP
                     FTRILSQPRVVADSLEDYLNRHPSYRMPVAGAPTPLLLTTGDADEVNRTARLFWPEVP
                     AFQALAP"
     gene            complement(17060..17929)
                     /gene="fabI"
                     /locus_tag="VFR00_02705"
     CDS             complement(17060..17929)
                     /gene="fabI"
                     /locus_tag="VFR00_02705"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HEU0158191.1"
                     /translation="MTNRDMAQAGPLMAGKRGLVMGVANERSIAWGIARMLAGHGAQL
                     AFTYQGDAFGRRAVPLAKSVGSEIIVSCDVLDLNSVDNALAEVKAKWGSLDFVVHALA
                     FSDRRELSGRYADTTRENFINTMVISCFSFTEVAKRAAALMPAGGSLLTLTFSGATRW
                     VPSYNVMGVAKAALEASVRYLAADFGAQAIRVNALSAGPMRTLAGAGVADARVIFNYQ
                     RDHAPLRRTPTLDEVGGSALYLLSDLSGAVTGEVHFVDCGYSTVAMPNLEFLKEQEQE
                     AAQAGRSPSEAAE"
     gene            complement(18034..19263)
                     /gene="fabB"
                     /locus_tag="VFR00_02710"
     CDS             complement(18034..19263)
                     /gene="fabB"
                     /locus_tag="VFR00_02710"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009796638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HEU0158192.1"
                     /translation="MRRVVVTGMGIVSSIGNNTQEVLASLREAKSGIVYAEKYAELGF
                     RCQVHGAPNLEWEAMVPRKARRFMEAGVGWCFVSMEQAIRDAGLDSKDVVNERTGIIM
                     GSGGPSTRAIVRAADVTREKDPKKIGPFEVPKGMCSGPSAALATAMQIKGTNYSISSA
                     CATSAHCIGNAAELIQWGKQDVMFAGGCEELDWTLSALFDAMGAMSTKFNAAPARASR
                     AYDKDRDGFVIAGGAGVVVLEELERAKARGAKIYAELVGYGATSDGFDMVAPSGEGAV
                     RCMRMAMAGVDGKGVGKVDYINPHGTATPVGDEREIDAIRQVFGKTLPPISSTKSLTG
                     HSLGAAGVQEAIYALLMMNNRFICESANIDELDPAFADVPISRKRQDDVNLNCVMSNS
                     FGFGGTNATLILRRYDA"
     gene            complement(19337..19873)
                     /gene="fabA"
                     /locus_tag="VFR00_02715"
     CDS             complement(19337..19873)
                     /gene="fabA"
                     /locus_tag="VFR00_02715"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019171 -
                     (3R)-hydroxyacyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-[acyl-carrier-protein] dehydratase
                     FabA"
                     /protein_id="HEU0158193.1"
                     /translation="MPERVATYEYADLLTCGRGELFGPGNAQLPLPPMLMFDRISNIS
                     EVGGAHGKGQVVAELKVSGNPALDWFFACHFKGDPVMPGCLGLDALWQLTGFFLGWLG
                     APGKGRALGVGEVKFTGMVMPTTKTVQYVVDLKRVILRRLKLAIADGVMKADGQVIYA
                     ATDLRVGLFAAGEQPAIS"
     gene            20137..20541
                     /locus_tag="VFR00_02720"
     CDS             20137..20541
                     /locus_tag="VFR00_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HEU0158194.1"
                     /translation="MGNSISQLLRRAGLRPTRQRIALATLLFGNGDRHVTAELLHEEA
                     VRAGERVSLATVYNTLHQFKGAGLLREIAIGGQRAYFDTNTSNHNHFFVEEQGRLVDI
                     PGDSIRVDGLPEPPPELKISHIDVVVRLVPRR"
     gene            complement(20585..21136)
                     /locus_tag="VFR00_02725"
     CDS             complement(20585..21136)
                     /locus_tag="VFR00_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016921733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEU0158195.1"
                     /translation="MGRIEVLAGMGLLVLVLCAGTGLAAQPASEPQGVGATGLALPRF
                     ASLKSDRVNLRQGPGTDYPTAWVFRRAGLPVEIVKEFESWRQVRDAEGTTGWVLGSML
                     SGRRTAVVLPWEQKAGQQQPASAALRDDDSATARPLAYVEAGVLASIISCDGRWCRIS
                     VGGFRGYIEQVKLWGAYQGEVIR"
BASE COUNT         3630 a         7196 c         7203 g         3380 t
ORIGIN      
        1 tcagcgcctt caacgctatc ctggatgggc tcgagcggcg cagcgactgg agcgtcctgc
       61 acctgcgcag cgcgctcaag gtattctcgg ccggcgccga cctcggcgag atcagcggcc
      121 acttcgccag ggcggataaa atggcagcgg ccgccgacct gacgcggccg ttccaggcgc
      181 tgttccagcg catcgccgac ctgccacagg tgagcctggc ggaaatcggc ggtgcggcca
      241 tgggcggcgg ctacgagctg gcgctcggct gcgacctgcg cattgccgcc aacgaggcca
      301 agctggggct accggaggtg gggcttggcc tgctgccggg cggcgggggt acacaacgtc
      361 tgacctggat cgcagggccg gccgtcgccg cgcgcatcat cctggctggc gacagcgtcg
      421 atggcgcaac cgcacgcgag ctcggcatgc tgcagtgggc ggtgccgggc gagacgctcg
      481 ccgccgaggc caaaaggctc gccgcgcgcc tggcggggct gccgcccttg gcgctggcgg
      541 cggctaaagt atgcatccgc ttggctcgcg accccgaacg caacggtttt gccgccgaga
      601 ccgagtggac gggccggctg ttggcaaccg aggagacgca gcggcgtgtc ggcgactttc
      661 tcgcgggcgt caggcgctag cggcttggca acgacaaagg cgccgctggc cgccagccca
      721 agcccgccgc gacccttagg gcggtgccgc cggcggccgc ggcgcaagac gagtgactga
      781 accggcaaaa cccggaggag aacgcatgct gctcaaattc aagacggtag tgctgaccgg
      841 cggcgcctcc ggcatcggcc gagccaccgt gctgatgctt gccagggagg gcgcgcgcgt
      901 cttgttgtgc gacgtcaatg cggcaggtgc ggcagagacc atcaagcagg cgcccgaggg
      961 cgccgtcgac tacgtcgcgc tcgatctggc ggacggcgct agcgtcgatg cctgcgccaa
     1021 aacggccctc gagcgggccg gccgaaagat cgacgtcttc atcaatgcgg cgggttggga
     1081 ccgcacgcag cccttcgtca acgccgacca ggccttcatc gacaaggtgc tcggcatcaa
     1141 cctcgccggt ccgctgcggc tcacgcgggc cctgttcccg gcgatgatcg aggccggcgg
     1201 cggcaagatc gtgttcgtcg ccagcgatgc gggccgcgtc ggcagcctgg gcgagacgcc
     1261 ctactcggcc gccaagggtg ggctcatagg cttcacgaag tccctggcgc gcgagggcgc
     1321 gcgccacaag ataacggtca actgcgtgtg cccgggtccg accgacacgc cgttgtttca
     1381 cgccaaccct gagcgcatgc gcgaggcctt gctcagagcc attcccttcc ggcgtctcgc
     1441 cacccccgac gatatcgccg gggccatcct ctacttcgcc ggccccagca gcgactacgt
     1501 gacgggccag atcctgagcg tgagcggggg cctgaccatg gcgggctagg caagcggccc
     1561 ccaagcgcgc acgcacgcgg gcgcggcgag ggcagcacct gagcgggagg caaaacatgg
     1621 agttcgacat ctggctgccg gcgcaccgca tcgcggcggc ggagaaactt tggcccggcc
     1681 gcctgcccat caacgacctc gacgctgccg tcgcccttgg ccccgagcgc acggcctttg
     1741 tcggctggaa cagccggctc ggccgccaga tccggctcag ctacggtgag ctcggcgagc
     1801 gcgtcgacaa aattgcccag gggctcgcag aactcggcat cgggcacggc gacgtcgttg
     1861 ctttccagct gccgaattgg tgggaattca ttgccctggt ctatgcctgc aaccggatcg
     1921 gggccgttgc caacccgctg atgccgatct tccgtcagcg cgaactgcgc ttcatgctgg
     1981 gcttcggcga ggccaaggtc gctatcgtcc cggcgacctg gcgcggcttc gatcacctgg
     2041 cgatgctgcg gcagatccgg gccgatctgc cccgcctcgg ccatgtcttt gccgtgggtg
     2101 gatcaggcga ggcctccttc gaggccgcat ttctcgagcg gcccccgctg acacgcatcg
     2161 agcaggcgag gctggccgag cgccagcgca cgcccaacga tgtggtcgag ctcatctata
     2221 cctcgggcac ctccggcgag cccaaggccg tcatgcatac ggccaacacg gtgctggccg
     2281 cggcgcaagg cttcgttgcg gatattcccg tcggccgcgc cgatgtggtg ttcatgggct
     2341 cgccttacgc gcaccagacg ggttttctct acggtatttt gatgccgatc atgctcgccg
     2401 gcaaaaccgt ggccctcgac cagtggtcgg cggccgcagc cgcgccgctg atcgaaagcg
     2461 aggctgcgag cttcagcatg ggctcgacgc ccttcctgtc cgacctggtg aacctgccac
     2521 aaatgctgcg gagcaaagtg agcaccacgc tgcggacctg ggtatgtgca ggcgcgccga
     2581 taccccgcct gttggttgag cgcgcgcgaa atgagatggc gctcgacgtt ctctcctgct
     2641 ggggcatgac ggagaatgcc ggcctcacca taacccgcaa gggtgacccg caagaaaagg
     2701 tgatcgggac cgatggccgg gcgctgcctg gtagcgaggt gcgcgtcgtc gatagcgcaa
     2761 aaaggccggt ggcgcccgat acggtcgggc atttgcaggc gcgcggaatt acgcatttcg
     2821 tcggttacct gaaacgaccg cagctcaacg gcatcgatga cgatggctgg ttcgacactg
     2881 gcgatctcgc gcgcatggac ggcgacggct atatccgtat cgtcggccgc agcaaggacg
     2941 tcatcattcg cggcggcgag aatatcccgg tggccgaggt cgagaacctt atttatcggc
     3001 atccccaggt ggccgaatgc gccatcgttg ccatgcccga cccacgcctg ggggagcgag
     3061 cgtgcgcctt cgtgatggcc aaagccgata cgctcatcac gcttcaagat ctgacgcgct
     3121 tcctcgatgg ccacggcatg gccaagcagt attggcccga gcgcctcgag cttgtggccg
     3181 agatgccccg cacgccgtcc ggcaaaatcc agaaattcaa gctgcgcgag cgcgcggccg
     3241 aactggcgcg cgggcccctc gcggcgaact gatcaggtct gcttggccac cgcccggccg
     3301 atgtggtctt ggatcagcgc ggcgagaacg gccgcgtggg tggtgatcat gaaatgggcg
     3361 gcgccgtcga cggttgccat cgccgagtgt ttcaggccct ggctgatgag ctcgttggcg
     3421 cgtttgacgg cgggatggct ggcgcttccg cgcagcacga gcaccggcag ctgcaaagtg
     3481 gcaagcatgg cgggggcaag ctgatagccg taggcactcg cccaatcgag gacgttgacc
     3541 gccgtcgttt cgacggcgta ggcccgcacc cgtggcggcc agccggcaaa ggttccgacc
     3601 cccgcataaa aatcgatcat ggtggcgatc gcctcggggt cgcccgcggc gaaggcggta
     3661 aagtaggcct gcgtcatgcc gcgaaacgca cggtagtgcg cgtgctcccc ggccgcctcg
     3721 agcagttggg cggcaggggc ctccacaatc gtcaggctga ccaagggcac gtattgcctg
     3781 agcgcgacgg cgagcgccgc caggccgccg aaggagtgcc ccaccagatg caccggcgag
     3841 ccggcgcggc gaatgaccgc ctcgagcgcg gcggcgacgt gggcgatgcc gggatcggcg
     3901 ttgctgcgcc gctcggccgt gccgccgtag cccggcaggc tcgtggtgat gcagcgaaac
     3961 tgcgcctcgc aatgggaaat gatcggccgc caggcggcac cggtactgca cgagcccggc
     4021 gcaaatacga cggtcgggcc gcgcccgcac tcctcgtagt cgacgcgtcc gcgcgggtcc
     4081 tcaatcgtca tgtcgtgtcg gaccacaggg gacgcgcggc tcacgtttaa aagtgcggcg
     4141 ggcatggctt actccggggt tggcgggctg tcacgtcgcg cccgcgggcc gcaggcgcag
     4201 ttgtcgccac ggccggcgaa taggccttga cggcaagggc tgtcgagggt tcagctttgg
     4261 gcaagctctg aacgaggatc cctcgacaat ggcgcagatc ttgacgacgc acgccgggag
     4321 cctgccacgg ccgccgctgc tgactgaact cttcacacgc cggtcgcaag gcgacagcgt
     4381 cgatgaggcc ttgctggcga cggccggccg tgaggcggtc gcctgggtgg tcgagaagca
     4441 gcgccaggcc ggcatcgaca tcatctccaa tggcgagcag cagcgcgaat ccttcgtgct
     4501 ctatttgcgg cgccggctga gcggcatcgg cggccgcggc gagcgggcgc cgtttgccga
     4561 tatcgaaagc taccccaagt tcaaagcgga gcgcgcccgc ctcttcgccg cgcgcccggc
     4621 cgtcagcaac gttgccttcc tgccaaagtg catcggcccc atcggctata ccggccacgc
     4681 cgagctcgaa aacgaatgcg ccaccttcaa ggcggccgtc gacggccttg gccgcagcca
     4741 ggccgcgccg ttcctgacgg cggcatcgcc aggcatcctc gcctccatcg tcaagaacga
     4801 gcactacgcc acattcgacg cctatctcga cgccatcgcg gcagcactgc gtatcgagta
     4861 cgagggcatc gtcgccgccg gcttccagct gcaaatcgat gcacccgatc tggcgctgga
     4921 gcgtcatgcc tcgttcgccg cacgaccgct gcaggacttc cagcacttcg tcgaacgcgt
     4981 gatcggcgcc atcaacgccg ccctcgtcaa cgtgcccgcc gacaaagttc ggctccacgt
     5041 ctgttggggc aattatgagg ggccgcatga tcatgatgtg ccgcttgccg atatcctgcc
     5101 cgtgctgttg caggccaacg tcggcaccct ctacctgccc ttcgccaatc ctcggcacgc
     5161 ccatgagtac aaagtcctgc agcagcagcc gctcaggtcg aaccaggtgc tggtggccgg
     5221 cgtcatcgat accgtgacca actacgtcga gcacccggag gtcgtcgccg accggctcga
     5281 acgcgtcgcg ttcgccttgg gcgatccgca gcgcctgctt gccgggacgg attgcggctt
     5341 tgatacctcg gcgggcatgg gacgcgtggc cgaggacgtc gtttgggcca agctgagcgc
     5401 cttggcggac ggagcccgcc gcgcctccca acgcctgttc ggctaacctg aagtcggcgc
     5461 agcggggggc ggcaacgctc gcgccgccgc cgcccgcaga gaccgctttg acgccgcagg
     5521 gcggcattgc ggctgctact tgttgcgtgg gtcgtccttg ggatcgatat attttacgtc
     5581 gaagggcccg aagccgttga tctgcacgat cgcctcgcca tccgcccagg catagtgggc
     5641 catgccggcc ggcaggtgca cgaagctctg cgccggcagc ggcttggccg ccgtccgatc
     5701 cagcttttcg ccggacgcga ccgcgaattt accggcgatg atcgtcagga actcgtcctt
     5761 ggagtgtcga tgcggcggca cggcaaagcc atcgggaaac ttgaggcgga tgacaaaggg
     5821 cccctcctgc gccggattgc cgagcaggat tgcggcctgg gccccgctcg gcagcgacgc
     5881 cggcgccgga ccccacttga ctgcgtcacc cggcaccacg gtgtggtggg cttggtccgc
     5941 cagggcaggt ccggcaagcg gggcagatcc gagcgataac agcaaaagcc aggccgcagc
     6001 gatcgatctt tgcgacatcg gtagggctcc ttcgagggcg cagcgcctcg cgcgctgcag
     6061 cgaagtcaac ccctagcgct tcgacatgga gatggccgtg gtaggcggcg tggaaacggg
     6121 catggaaaac gtgcgaaggc ggaccctgcc gcgccctggg accgcgacgc gcgggccggt
     6181 tgcggctcag gcctcaaggc cgcaacgccg catgcaggcg cgcaagcgta tcgatggtcg
     6241 agtgatcgat tttgccgtcg acccgggcgg gacggaagtg gcgttgaaag gccctgatca
     6301 cgcactcggt ggggccgtcg acgacgcccg tcggctcgat gccatagcca tactcgcgca
     6361 gcagcagctg tagctcggca aactgaggac cggcggcccc gacgccgatg ccgggttcgg
     6421 cggcgatgag cggcgcaggg tcgacccaat ggccgacgcc ggcggcggct agccggctcc
     6481 agggaaattt ctcgccgggg tcgatcttgc gcctgggggc gacatcggaa tgggccagca
     6541 cgcgctcggg ccggatgcga tggcgcgcga ggatgtcccg cgccaaggct tcgacggcgt
     6601 cgagctgggc ggcggggaaa tccggataac cgaactcgtg cccgggattg tgcagttcga
     6661 tgccaatgga ggcggagttg atatcggttt cgccggccca acaggcctcg ccggcgtgcc
     6721 aggcgcgcgc cgcctccgcc accatttggg tgacgcagcc gcactcgtcg accacgtaat
     6781 ggcaggacac gcggctctct gggcgcgcga gccaatcgat cgccttggct gcggtggcaa
     6841 cgccggtata gtgcaagagc agaatgctgg gccgcatacc cccgcgtcgc gcctcgaagt
     6901 tcgcggccgg atgcagctcg cgcacgaggc ttgaatcggc cttgaagatc aaagcccgcg
     6961 ctccttggcg atgctgtcgt aggcggcatt gatggcggca agcttgcggg tggcgagatc
     7021 gacgaactcg gccggcaccc cgcggccgat cagcttgtcg ggatgattgt cggtgacgag
     7081 cttgcggtat tgcgcgcgga tctcggtgtt gctggccgag gggggaagct tcaggacgtc
     7141 atagggattg tcccggtccc gcacgaaacg ggcgcggaaa taacgatact cgttgtcggc
     7201 aaaaccgaag cgctgcgcta ccgctttgag aaactgatcc tccttgggat gcaaggcgcc
     7261 gtccgccgcc gctacgtaga gcaggccctc cagcacatgc tgcaagagcc ggcggtcgtc
     7321 gcccaacagc gcggcgatct gatcggcgta ggcctcgtag ccggcggtat cctgcttggc
     7381 gaggtcgaac acgcgcgcca cgttggcggc ctcctccgcc gagaattgaa agacgcgctt
     7441 gaaggcgtcg acttcgcgtt ccgacacgac gccgtccgcc ttggccattt tggcgctgag
     7501 cgcaattacg ccgatggtga aggcgacgct cttctccggc gcgccgctgc ggtcgaaacc
     7561 gaagacgctg gccacttcgt gcagcaggct gccgccggcg tcgccgagca ccgtggcaaa
     7621 accggctatt ttttgccaaa ccgtcattcc caagcctcag gcgtccctgg cggagcaaaa
     7681 agcgttgccg ccgccccagg ggagggctaa tcccccaggt tagaacgcct tgtcaaggat
     7741 cgagcatgag cgaaacacca aaggatgagg tgctgatccg ccgcgagggg cgggcggggc
     7801 gcatcaccat gaaccggcca caggcgctca atgcgctgac gcacgccatg gtgggaccga
     7861 tcttcgaggc cctcaaggcc tggtgcgctg atcccggcgt cgaattggtc gtgctggacg
     7921 gcagcggcga gcgggcgctg tgcgccggcg gggatgtgcg cgcgctctat gacagccgca
     7981 gccgcggctc gggcgccgcg cgtgccttct ggcgcgacga atatcgcctc aatgccctga
     8041 tcggtcgcta cgcgaagccc tatgtggcga tccaggacgg catcgtcatg ggcggcggca
     8101 tcggcttgtc cgcacacgcg cgccaccgca tcgtcacgga gcgctcgcgg ctggccatgc
     8161 cggagacggg catcggcctc atccccgatg tcggcggcag ctggctgttg gcgcacgccc
     8221 cgggcgaggc gggcgtctat ctcggcctga cgggtgccga gatggatgcg gctgacgcga
     8281 tccatgcacg ctttgccgat catttcgtgc cgtcgagcgc gctcggcgag ctcaaggcgc
     8341 gtttgctcga tcccaggggc ggcgagccgg gcgagcttct caaacgcttg gcggcgcccg
     8401 tggcacgctc ccgcctagcc gagcgcgggc ccgcgattga ccatattttt gccggtgcga
     8461 gcgttgaggc gatctgcgat cggctggcca agagcgacct cgactggagc caacacacct
     8521 gcgcggcgct ggcgcagaag tcgcccaagg ccttgaagct tacctgcgcc gccatccggc
     8581 aggcgcgcac cctgccctcg ctcgaggccg ccctcgacgt cgaatatcgg ctggcggtgc
     8641 ggctgttcga ggacggcgag ttccccgaag gcgtgcgcgc cttgatcgtc gacaaggacc
     8701 gcaagccggc ctggtcgccg tcaactctgc agcaggtgac cgatgcgctg ctgcagcgct
     8761 atctggcacc gctgccggcc gcggaagagt tgggcttggc gccggcccgc acgcaaaact
     8821 gaggtgaggg accgcggcgc gccggcgggc tattcggcgg cctgagcaat ggtgggggca
     8881 tcgcccgcca ccgtcgtgcg gcgcacgtcg cgcggctggc tttcgtcgta acgtcgaccg
     8941 cgatgcatgg tctggcggtt gtcccacatg acgaggtcat tgaccttcca cttgtgcgcg
     9001 tagacaaagc gcggctgcgt ggcgtgctct ttcagatcat gcaacagaat gcgcgcctcg
     9061 ggcgtcggct tgccgatgat gccgccggcg tgggaggaca gatagagcga cttgcgcccg
     9121 gttacgggat gggtgcgcac caggcgctgg cgcaccggct tgaagagctg cttttcctcc
     9181 tcggtgtagt cgacaaagcc cagcgagccg cgcgagtaca tcaacgagtg ctcacagacg
     9241 agatcctcga tctcggcctt gctatccgca tcgagcgcat cataggcggc gcgcatgtcg
     9301 gcaaattccg tatcgccgcc cttcgggttt acgacgcgcg ccgaaagcag cgaatacttg
     9361 gcggggatcg ggcgaaagga gctgtcggag tgccacagca ggttgcccag attgaacagg
     9421 cgctggcgac tgtcgcgcgc cagcggcctg ccatctttgc cgagattgga gacgtcgttc
     9481 atcccggtgg cgagccgctt gtcttcctct ttggtgacat tgccgccgcg ggcgttctcg
     9541 atcgccccga agttgcgggt gaataccatc tgctgttcgt cgctcaccgc ctgaccgggg
     9601 aagacgagga cggcatagcg gtccatgccg gcctcgatgg ccgcggcgtc ggccggcgac
     9661 accggccggc tgatgtcgat cccggagacc tcgccgacga acacggggtg gagctggcga
     9721 atggcgacgg gcacggctga cattccttct gttctggagg cgcttaaggg cagatcgcgc
     9781 ggaagcctag ggcaggcacg agcgtttgaa aagtgaggca acgcccttgc caagcggtgg
     9841 cgccttgctg cgggggcgag ccttggcggc gggctgccgt gggccgaggc ggggcggcct
     9901 cggcgctcgc cgggccgccg ggcggcaccc tgacggcgcc ggcgcgaccc aagcgagggc
     9961 ctgggttctc aagggcggac ggcaaccctg ggacatcgcc tgctcgcgcc catgcacccg
    10021 agcccccacc gcgtccagcg cggcgggcaa gttcgttaac tttttgcggg gctcgcggtc
    10081 acgcgctgtc tcgaaatgaa acaggtccgg gtgcgagggc cgccgcgcgc gtccaattta
    10141 tgtctgccat gaaataaacg tgatctggcg ccagtttggc gcattgtccg acggcatcgg
    10201 gggtggcgcc ggaggctttg ctcggagctg cccctgcggg cgcggctccg gctcaaggca
    10261 aggcaggttt caacgctacg gtttcaacgc tacgggcggc gcatcgcgtc cgagccgtca
    10321 gcatggtccc aggcgatttc tgccgtcgcg aaccgggcgc cccctccgag gcgttggaag
    10381 ctcgccagct ggcatagccc gtggcgaagc tgccgacgcg tgccgagcaa caagggcatc
    10441 gaagggctcg aggaggcttt aacgtgtttc gttttgctat cacgctcggt taccttgcct
    10501 gcctggccat gctggccacg cccgccaccg cgcagctctt gtggccggga acggcggaag
    10561 tggcgcaggc caccacgccg cggcgcgctc aacccattcc caacaagccg gtcagtgaag
    10621 aagaggcggc aaaggccgcc cgtatcaaca actggaccgt cggtgtcgcc ggtggcctgt
    10681 tggaaggcac cttcgttcgc tacgcggccg atctgggcaa agccctcgat gacgcggaca
    10741 acctgcgggt tttgccgatc gtgagctacg gggcggtcgg caatgtcagc gatctcatct
    10801 atctcaaggg cgtcgatatc gccattacct actccgatgt gctcgatcac tttcgcaacg
    10861 tggaacgcat ccccggcatc gagcggcgcg tcaactacat catccccatg tttcagggcg
    10921 aggtgcacgt ctacgtgcgc gaggagatca agagcttcca ggacctggtc ggcaagaagg
    10981 tgaacttcaa caccgtcggc agcgccgcca actacaccgg cggtatcgtc ttcgatcgcc
    11041 tgggcatcaa ggtcgaacgc ctgttccaga acaactcggt cgccctcgag caaatgcgca
    11101 aaggggagat cgccggcatc gtgcacgtgg tcggcaaacc caacgacctc ttcgccaagt
    11161 tcaagccgga gcccggcttt cattttctgc ccgtcgaatt cggcgccaaa ttcgaagatt
    11221 actacgtgcc cgccgagctg actgccgccg actatcccaa cctgatcgcg cagggtgaaa
    11281 ccgtgcagac catttcggtg ccggccctgc tcgcggtcta taactggaac aaggataccg
    11341 atcgttaccg gcgctgcgtg cgctttgtcg agtatttgtt cgaccgcttc gagaagctgc
    11401 gcgacaagcc ctaccagccc ggctggaagg acatgaacct ggccgggacg atcccgggct
    11461 ggacccgctt cccgccggcc gaggccttgg tcgccaaggc cgcggcgcag gcgagcgcgg
    11521 aggccaacct gagccgcgcc caggccacac gtgctaccgc cgacaacaag gccgaacagg
    11581 agcgcctgtt caagcaattc ctggagtgga gcaagcagca gaagcgacgc tgaggcgggc
    11641 gccccagcgc cgctcccgaa ccggggggcg ggcagggcaa agccgctgag gacaagaccg
    11701 gcccagcgcg gtcagcaagg tgtggcgggg tgcgtcgacg gcgatcccgc cgaggggaaa
    11761 cgacgaagga ggcgccagcg cacacgccac ttgaggtcag cctccctggg gattggggag
    11821 ctggccagtg cgggccgcgt gcacaggcga ttttttttgg tgcgtgttcg atcaagactt
    11881 gccggcatcg cgcttctttg cggcgccctg ctgatggtgg cgcccagcgg gcggcctggg
    11941 ccacatcctg ctaccgccca gcagggccag cccgtcagcc tcgacgtggc agcggtgacg
    12001 aacgccgcgc cggcctcgcg tgtgcgcctg ccgatcgagg tccgaccacg cgacgccctc
    12061 gccaagaact cgttcattcg cattcgcggc ttgccggcaa cggcagccct gtcggaaggc
    12121 tatgcgatcg cgcccggctc ctgggccgtc ccgctcaacg gcctggcaag cctgatggtc
    12181 attctccccg tcgggctcga aggccggtcg gaggtcgtcg ttgctctcgt caacgtcgag
    12241 ggctcgatcc tggccgaggc gacgacgacg ctggtcgtcg cccgcgccgc cacacagccc
    12301 ccgcccccgg cgccggttgc cgcagcaccg cccaacagca cgacgcgggc gctgtcggca
    12361 ccccagctcg cgcccgccga tcgggaacgc gcgctgaaac tgcacgcccg tggccaggaa
    12421 cagttgggtc ggggcaacat ctatgccgcg cggcagttct tcgaacgcgc cgccgaggcc
    12481 ggcctggcgg agagcgcctt ggcgctcgcg gccacctatg atcccaccga gcttgctcgg
    12541 ctcagcgtct tggggctgaa agcggaaccg gccgaggccc gcaagtggta tgagaaagcc
    12601 cgcgagctgg gcgcgagcga agccgacgcg cgcctgcgtc gcctgcccgc gcgctaacgc
    12661 catggggcgg gtccgagccg cagtcgccac ggcgcacccc attgactaat ggggaatttc
    12721 ccacgagagt gcgggaatgc acaccctggc ccgcgcgctg cttgccgaga tcgaagcctt
    12781 tctggcgcaa agcgggctga cgccgaccaa attcggcctg gccgcggtca acgatggcca
    12841 tctcgtcgcg aatttgcgca agggcaacag cgtcacgctc aagaccgccg accgcgtgcg
    12901 tgcctttatg cgtgagcgcg cgcgcggcgg cgcgccggcg aacaccccgg caccgcccgc
    12961 cctgctgcac gccgcggcac ggagcctgac gccggggcct gcggcttggc cttttgccgg
    13021 gcccgatggg cggcggcgga tcgtgctcat catcggcggc ggcatcgccg cctacaaatg
    13081 cctggagctg atccgccgcc tgcgcgatca gcacgtcgag gtgcgagcca tcatgaccag
    13141 agccgcgcag gccttcatca ccccgctgtc cgtcggggcc ttgacgggca accgcgtctt
    13201 taccgaactc ttcgacctca acgaggaaca ggaaatcggc catatccgcc tggcgcgcga
    13261 agcggatctg atcgtggtgg cgcccgccac cgccgacctc atcgccaaaa tggcgggcgg
    13321 gcatgccgac gatctggcct cgtgcgtctt gttggcaacc gatcgacctt tactgctggc
    13381 gccggccatg aacccgcgca tgtggctcaa ccccgcaacg cggcgcaacg tggccctgct
    13441 tgaggccgac ggtatcggct tcgtcggacc gggggtcggc gagatggccg aagccgggga
    13501 ggcggggccc ggtcgcctgg ccgaagtccc cgatctgctg gcggcgatca tcgccaggtt
    13561 ggggaacgtc gcctcgccag gcgaggcgca gggcaatgat cgccgcggcg gcgacagctc
    13621 ccagccgagc ttctcgccga ccagcggggc aaccggtcca ctcgccgggc gacacgtcat
    13681 cgtcaccagt ggtcccacgc acgagccgat cgatcccgtg cgctacatcg ccaaccgctc
    13741 gtccggcaag cagggcacgg ccattgcggc gcaagcgcgc ctgctcggcg cccgggtaac
    13801 gctcgtctcc ggcccaagcc agcttgccgc ccccgccggt gtcgacctgg tgcgcgttga
    13861 aacggcgcaa gagatgctgg cggcggtgca gcaagccttg ccatgcgaca tcgccatctt
    13921 cgccgccgcc gtcgcggact ggcgcgtggc cgagacgttc ggcgaaaagc tgaaaaaggg
    13981 cgccgccgcg acggcgccgc ggctcgagct cgtcgagaac cccgatatcc tcaagaccat
    14041 cgcggcaacc gcgccgggac gacgaccacc gctggtgatc ggctttgccg ccgagaccac
    14101 gcaagtcgtc gactatgcca aaaagaaacg caaggccaag ggcgccgact ggatcctcgc
    14161 caacgacgtc tcgccaaaaa ccggcattat gggcggcgac cgcaacacgg tgcatctcgt
    14221 gacggcgacc gacgtggagg attggcccga cctgcccaag gcggaggtcg cgcggcggct
    14281 cgtcgcgcgc gcggcgcagt ggctcgccga acataccgcc ctgacatgag ccccaagtct
    14341 acccggcagc gctcgctggt cggcgtggtg cgactggccc acgcgcgcgg cttggacttg
    14401 ccgcgctatc aaagcgacgg cgcggccggc atggatctca ttgccgccat cgccgagcgc
    14461 cggccgctcc ggctcgcccc gggcgcgcgt gccctgatcc ctaccggcct ggtgctggag
    14521 ttaccggcgg gcttcgaggc gcaggtgcgg ccgcgttctg gactggcgct caagcacggg
    14581 ctcaccgtgc tcaacagtcc gggtacgatc gatagcgact accgcggcga ggttaaggtg
    14641 ttgctcgcca accttggcgc cgccacctgc gccatcaaac gcggtgagcg catcgcccag
    14701 ttggtgttcc agcgcgtcga acgcgtcggg ctgcgagaga tcggcgaggc atcgccgaca
    14761 ccgcgcggcg ccggtggttt tggttcgacc ggccgcaaac atcgaggcgc cgaggccgaa
    14821 ccgcgcgccc agcggcgcca gcgcgcgaaa acttccgcgc gctgagcggc gcacattaca
    14881 gcgcgttaat cgtcatacag agcgttacaa caagatccgg ccgcagagcg accgcgcttt
    14941 cgccgcgcgg ggatcccggc gcagtcaaaa aatgctgcta gtgagcgatg tctgacagtc
    15001 ccagagaaag ggttgagaca tgcgcttctg gtcagttgct cctgttgcgg cggcaacctt
    15061 cgccgcgatt ggcgccgcgg gtggcccgtt gctcgccgag caggctcctc gtctatcagg
    15121 cccccacgcc tacgaaaatt tggccatcta cttcgtgcac ggcacaagcg ccgctggcac
    15181 cgtccccctc acgctcgagg agggtgttgc caagggccgg gtgcaggtga tcgaaacggg
    15241 tcgggtcaac gagctgatga tcgaaaacac cggtaccgag ccggtgttcg tgcaggccgg
    15301 cgacattgtc aaaggcggta aacaagatcg ggtgctgacg gtgagcctgc tgctgccggc
    15361 caattccggc cgcctcccca ttcaatcctt ctgcgtcgaa caggggcgtt ggacggcgcg
    15421 cggcaaggaa gacgcaaaca agttctcgag cgcgaccgag gccatgccgt cgcgagccgc
    15481 gttgctggcg atggcagccc ccgcgccggc caaggagagc ggctatcgtg ccgccggcga
    15541 gcggccggac ggcggcgacg tcgccggcaa gcaaaagaag gtttgggatt cggtggcggc
    15601 aacgcagagc aagctggcgg acggggtcgg cgccagcgtc aattcgcccg cctcgggaac
    15661 gagcctgcag ctcagcctgg aaaacgagaa gctgaaggga gctcgcgccg cctacctcgc
    15721 cgcgctcaaa ggcaagggcg aagccgaggc cgacatcctg ggctatgtgg tggcgatcaa
    15781 cggtaaggtg agcagcgcca acctctatcc atcgaacgcg atgttccgca agatgtggaa
    15841 caagcaattg gcagccgtcg tcacggaggc gatcggcgaa aagaaagctg ccggcgacgc
    15901 gaccccgcca cccgccccgg cgcaggccgc cgagttcctg gccgaggccg agcgtggcaa
    15961 gccttccgcc cgcgcagacg cgatcggcac gcgccaggag acgcgcgatg gcgacaagac
    16021 gctctttaac gaggcaaaaa gcaacgcagg ggcatggctg caccgcagtt atctcgccaa
    16081 gtagcgcgcg ccagccgcgg ttgaggccgc ggctggtcgg accaggcgat tgggatctgg
    16141 ccggtcgcgg ccagatccta aggcgcgagc gcttgaaagg ccgggacctc gggccagaac
    16201 agacgcgcgg tacgattgac ctcgtccgca tcccccgtcg tcagcagcag gggcgtcggc
    16261 gccccggcga ccggcatgcg gtagctgggg tgccggttca ggtagtcttc caagctgtcg
    16321 gccaccacgc ggggctgcga caggatgcgg gtgaacgggg gcaagtgccg gcgaaagagg
    16381 tgttcgatca gcggatagtg cgtacagccg aggatggcac ggtgcggtgg cgctcccgcc
    16441 gtttgcgcca acagaccggc gacggcatcg gcgacgagcc cctccagggc cgcctctgcg
    16501 gcgccggcct cgatggcgcc ggcaaggccg gcacaggcct gctggatgac cgtcacccgc
    16561 gggcagcgct tgtggatttc ttccgggtag gcatgcgagg cgatggtgcg cgtcgtcccg
    16621 aacacggcca cgacgtcggt gttgtacttt tgcgggtact gcggggtcgc caccgcccag
    16681 ggggtctggg tcgccgcctc cacggtcggg gcgacgatgc ccaagacgtt gtgaccctgc
    16741 cagccgctga cgggcagcca gccgcgctgc agcgcgcgcg ccgccacgca ggtggcggtg
    16801 ttgcagccga gcagaacgag ccggcagcct cgcgcgaaca ggcgctcgac gccctgccgc
    16861 gtcaaggcaa cgatgtcgtc ggacgcgcgc tccccatagg gcacgtgggc gtggtcgccc
    16921 aaatacacgt aggcgaggtc gggaaagcgc cgggccagct cacgcacgat ggtgagcccg
    16981 ccatggcctg aatcgaaaac cccgatcatg cgtcgtgctc agacgccggc cgacccggcg
    17041 agggaaaaat gaggggcggc tactcggccg cctcggacgg cgaacggccc gcctgcgccg
    17101 cctcctgttc ctgttccttc aggaactcga ggttcggcat ggccacggtg ctgtagccgc
    17161 aatcgacaaa atgcacttcg cccgtcaccg ctccggacaa atccgatagc aagtaaagcg
    17221 ccgagccgcc aacctcgtcg agggtaggcg tgcgccgcaa gggcgcgtga tcgcgctggt
    17281 agttgaagat cacgcgggca tcggcaacgc cggcccccgc gagcgtgcgc atgggcccgg
    17341 ccgagagcgc attgacgcgg atcgcctgcg ccccgaaatc ggccgccaaa taccgcaccg
    17401 aggcctccag ggcggccttg gccacgccca tcacgttgta ggaaggcacc cagcgcgtcg
    17461 caccggaaaa ggtcagcgtc aatagcgagc cgccggccgg catcaaggcg gccgcgcgct
    17521 ttgccacctc ggtgaagctg aagcaggaga tcaccatggt gttgatgaaa ttctcccgcg
    17581 tcgtatcggc gtagcggccg gacagttcgc gccggtccga gaaggcgagc gcgtgcacca
    17641 cgaagtcgag actgccccac ttggccttga cttccgcaag cgcgttgtcg acgctgttga
    17701 ggtcgaggac atcacagctg acgatgatct ccgagcccac cgactttgcc agcggcacgg
    17761 cgcggcgtcc gaaggcgtcg ccctgatagg tgaaggccag ctgcgcgccg tgcccggcca
    17821 gcatgcgcgc gataccccag gcgatcgagc gctcattggc cacgcccatg acgaggcccc
    17881 gcttgcctgc catcagcggc ccagcctgag ccatatccct gttcgtcatg acctcattcc
    17941 ttttgcggtc tcagcgggca actgcccgat tgacaagcct ccgacctgcc cctctgaaaa
    18001 cgccggcgat cccagccatc gcggcaaatt ttctcatgcg tcgtaacgcc tgagaatgag
    18061 cgtggcattc gtcccgccga atccgaaact gttcgacatc acacagttaa gatttacgtc
    18121 gtcctggcgc ttgcgcgaga tcggcacatc ggcgaaagcg gggtcgagct catcgatgtt
    18181 ggcactctcg cagatgaagc ggttgttcat catgagcagc gcgtagatgg cctcctgcac
    18241 ccccgcggcg cccaacgagt ggccagtcag cgacttggtc gaggagatcg gcggcagggt
    18301 cttgccgaac acctggcgga tggcatcgat ctcccgttcg tcaccgaccg gcgtggccgt
    18361 gccgtgcgga ttgatatagt cgaccttgcc cacgcccttg ccgtcgacgc cagccatggc
    18421 catgcgcatg cagcgcacgg cgccttcgcc ggaaggcgcc accatgtcga aaccgtcgga
    18481 ggtggcgcca tagccgacga gctcggcgta gatcttggcg cccctcgcct tggcgcgctc
    18541 gagctcctcc agcacgacca ctccggcacc ccccgcgatc acgaagccgt cgcgatcttt
    18601 gtcgtaggcg cggctggccc gcgcgggggc ggcgttgaac ttggtggaca tcgcccccat
    18661 ggcgtcgaac agggccgaca gcgtccagtc gagctcctcg cagccgcccg cgaacatcac
    18721 gtcctgcttg ccccattgga tgagctcggc ggcattgcca atgcagtggg ccgaggtcgc
    18781 gcaagccgac gagatcgaat agttggtgcc cttgatctgc atggcggtgg ccagcgcggc
    18841 cgacgggccg gagcacatgc ctttgggcac ttcaaatggg ccgatctttt tcgggtcctt
    18901 ctcgcgcgtc acatccgccg cgcgcacgat ggcgcgcgtc gaaggtccac ccgagcccat
    18961 gatgataccg gtccgctcgt tgacgacgtc cttgctgtcc aacccggcat cacggatcgc
    19021 ctgctccatc gagacgaagc accagccgac accggcttcc atgaagcggc gcgccttgcg
    19081 cggcaccatc gcctcccact cgaggttggg ggccccgtgc acctggcaac ggaagccgag
    19141 ctcggcatat ttttccgcgt agacgatgcc ggatttggct tcgcgcaacg aggccagcac
    19201 ttcctgggta ttgttgccga tcgaggacac gatacccatg ccggtgacga ccacgcgtct
    19261 cattgccaga cctttcttgg catcgtcgtg ccgctcgtcg ctatgccaaa cccgcacacg
    19321 gaggaggacg cacccttcag gagattgctg gctgctcgcc ggctgcaaag agcccgacgc
    19381 gaagatcggt cgcagcatag atcacctggc cgtccgcctt catcactcca tcggctatgg
    19441 caagcttcaa gcggcgcaag atcacccgct tcaggtcgac gacgtactgc acggtcttgg
    19501 tggtgggcat gaccatgccg gtgaacttca cctcccccac gcccagcgcc cgacccttgc
    19561 cgggcgcgcc gagccagcct aggaaaaagc cggtgagctg ccagagcgcg tcaagcccca
    19621 ggcagcccgg catcaccgga tcacccttga aatggcaggc gaagaaccag tcgagcgcgg
    19681 gattgcccga caccttgagc tcggctacca cttggccctt gccgtgcgcc ccgccgacct
    19741 ccgagatgtt ggaaatccgg tcgaacatca gcatcggtgg caggggcagc tgggcattgc
    19801 ccgggccgaa gagttcgcca cggccacagg tgagcagatc tgcgtattcg taggttgcaa
    19861 cgcgctcggg catcgccctc cctctacgaa atgtcggtgc gcgaacgcca cttgcgctct
    19921 atttacccac gcctggcatg tcctatcaca ctcgcccccc gctcaaaagc ccgtgttccg
    19981 tgcccaaaag gccacgaaac ggccgccacg tggacgttgc tccgcccggc acggcgacat
    20041 atacttgcaa agaacggcaa attcaaagtt gcgggacggg tttgccgcga taggcgagcg
    20101 cgcttggcgg aggtccccac cggcagagcc tgtgctatgg gcaattctat ttctcaactc
    20161 ctgcgccggg ccggccttag gccgacgcgc cagcggatcg cgctggcgac gcttttgttc
    20221 ggcaacgggg atcggcacgt gaccgcggaa ctcctgcacg aggaggccgt gcgcgcgggc
    20281 gaacgcgtgt cgctcgcaac cgtgtacaat acgctgcatc aattcaaagg cgcgggcctc
    20341 ttgcgcgaaa tcgccatcgg cggccagcgc gcctacttcg ataccaacac ctccaaccac
    20401 aaccattttt ttgtcgagga gcagggccgc ctggtcgaca tccccggcga ctcgatccgg
    20461 gtcgatgggt tgcccgagcc gccgcccgag ctgaaaattt cgcacatcga cgtggtggtg
    20521 cgcctggtgc cgcggcgctg aggcctggcg cgcagccgcg gctgggggcg cccgagcccg
    20581 agccttatcg gatcacctcg ccctggtagg caccccagag tttgacctgc tcgatatagc
    20641 cgcggaagcc accgaccgag atccggcacc agcgcccgtc gcagctgatg atgctggcca
    20701 gcacgccggc ctcaacgtag gcgagcgggc gcgcggtcgc gctgtcgtca tcgcgcaagg
    20761 ccgccgaggc cggctgttgc tggcccgctt tctgctccca gggtaggacg accgcggtgc
    20821 gccgaccgct gagcatcgag cccagcaccc agccggtggt gccttccgca tcgcgcacct
    20881 ggcgccagct ctcgaactcc ttgacgatct cgaccggcag gccggcccgg cggaacaccc
    20941 aggccgtggg gtagtcggtg ccggggccct ggcgcaggtt gacccgatcc gacttcaggc
    21001 tggcaaagcg cggcagggcc aaaccggtgg cgccgacgcc ctgcggctcg gatgcgggtt
    21061 gggcggcaag tccggtgccg gcgcacagca ccagcacgag taggcccatg cctgccagaa
    21121 cctcgattct acccatgcgc ggatgcctga accgcccccc ttcttccacc tgccgcggga
    21181 aagcacgatt ttgcgtcgcc gatcaagccc ctcccgatcg gcgggggctt ggcgtcgggc
    21241 caccttgtcc ccaccacggt atctgctagt acagggcaag gcgtttgccg ccagggtgga
    21301 gcatcaattg agagccgcgt gagccccgca tgcccaatcc aagcaaaaag ccgctggtga
    21361 tcgtgacccg caaactgccc gaggtggtgg aaacgcgcat gcgcgagct
//
LOCUS       DASTMT010000127         1505 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_1380377, whole genome shotgun sequence.
ACCESSION   DASTMT010000127 DASTMT010000000
VERSION     DASTMT010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 1505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1505
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1380377"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..477
                     /locus_tag="VFR00_02730"
     CDS             <1..477
                     /locus_tag="VFR00_02730"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU0158196.1"
                     /translation="SIPEGGIEAAVGIARGTSNCKLVVLTALDDVMSVSKALSAGVKG
                     YILKGISGTELVAAIKSIHRGLPFVTPELASRLLTDAKGGALLPMREAKLQAALSYRE
                     QQILLHVSKGLTNKEIADRLGLTVGTIKHYVTQLFKKMNVRNRLEAIQVAQKSDLT"
     gene            complement(496..>1505)
                     /locus_tag="VFR00_02735"
     CDS             complement(496..>1505)
                     /locus_tag="VFR00_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009490790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HEU0158197.1"
                     /translation="RIGDLQALAYAGRRRALFEYWGHEASLLPVALQPMLRWRMARAS
                     RGEGIYKTLATFARERGELIAQVQREIADRGPLRAGELSHQHKGDGGWWGWSDGKRAL
                     EWLFWTGAVSTATRYETFERAYDLSERVLPKAIVEAPTPAEAEAQRHLLRIAAGALGI
                     ASERCLRDYFRLPVADARARIAELVEAGDLLPIAVQSAFPSAYLYRHARHPRRMAAQA
                     LLAPFDPLIWQRQRAELLFGARIRLELYTPPEKRVHGYYVLPFLLGDRIVARVDLKAE
                     RARAVLRVQAVHGEAGIATAVVEPLALELRLMARWLGLHRVEVRPAGNLARPLAAAVR
                     D"
BASE COUNT          289 a          497 c          493 g          226 t
ORIGIN      
        1 agcatcccgg aaggcggcat cgaagccgcg gtcggcattg cccggggcac atcgaactgc
       61 aagctcgtcg tgctgacggc tctcgacgac gtcatgagcg tatcgaaggc cttgtccgcc
      121 ggcgtgaagg gctacatcct caaaggcatc agcggcacgg agctggtggc cgccataaag
      181 agcattcacc gcggactacc gtttgtcacg ccggaattgg cttcgcggct cttgacggat
      241 gccaagggcg gcgccctcct gccgatgcgc gaggcgaagc tgcaggcggc gctcagctac
      301 cgtgagcagc aaattctgtt gcacgtctcc aaggggctca ccaacaagga aatcgccgac
      361 cgcctcggct taactgtcgg caccatcaag cactacgtca cccaactttt caaaaagatg
      421 aacgtccgca accggctcga ggccattcag gtcgcccaga aatccgacct gacgtgaggg
      481 tagcgcacag ggccctcaat cgcgcacggc tgccgcaagg gggcgcgcca ggttgcccgc
      541 tgggcgcacc tcgacgcggt gtaagccgag ccagcgggcc atcagccgca actccagtgc
      601 caacggttca acaacagcgg tcgctatccc cgcctcgccg tgcaccgcct gcacgcgcaa
      661 gaccgcgcgc gctcgctcgg ccttgaggtc gacgcgtgcc acgatgcgat cgccgagcag
      721 gaacggcaag acgtagtagc cgtgcacgcg cttctcgggc ggcgtataga gctcgaggcg
      781 aatgcgggcg ccgaatagaa gctcggcccg ttggcgctgc caaatcaagg gatcgaatgg
      841 agccagcaag gcctgcgccg ccatccgcct agggtggcgc gcgtggcgat agaggtaagc
      901 ggacgggaag gcggattgca cggcgatagg gagcaagtcc cccgcttcga ccagctcggc
      961 aatgcgcgct cttgcatccg cgaccggcag gcgaaagtag tcgcgcaagc agcgctcgct
     1021 ggcaatgccc agggcgccgg cggcgatgcg cagcaggtgg cgctgcgcct cggcctcggc
     1081 gggcgtcgga gcctcaacga tggctttagg cagtacgcgt tcgctcagat cgtaggcgcg
     1141 ctcgaaggtt tcatagcgcg ttgccgtcga caccgcgccg gtccagaata gccattcgag
     1201 ggcgcgcttg ccgtccgacc aaccccacca gccgccgtcg cctttgtgtt ggtgcgaaag
     1261 ctcgccggcc cgaagtggac cgcgatcggc gatctcgcgc tgcacttggg cgatgagctc
     1321 cccgcgctcg cgggcaaagg tagcaagcgt tttatagatc ccctccccgc ggctggcgcg
     1381 cgccatgcgc cagcgcaaca tcggttgcag ggcaacgggc aaaagggacg cctcatggcc
     1441 ccaatattca aaaagggcgc ggcggcggcc ggcgtaggcc aaggcctgca aatctccgat
     1501 ccgat
//
LOCUS       DASTMT010000128         1572 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_4630231, whole genome shotgun sequence.
ACCESSION   DASTMT010000128 DASTMT010000000
VERSION     DASTMT010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 1572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1572)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1572
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4630231"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(5..490)
                     /gene="tatB"
                     /locus_tag="VFR00_02740"
     CDS             complement(5..490)
                     /gene="tatB"
                     /locus_tag="VFR00_02740"
                     /inference="COORDINATES: protein motif:HMM:TIGR01410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HEU0158198.1"
                     /translation="MFDITSSKLLILGIVALLVIGPKDLPALLRTIGKYVGIIKRQAA
                     EFRAQFDEAIRESEFADLKKDVESLGREAEQSMRAAEQSVEKDWAQAQESVSAALEDR
                     AGAPAAEPPATPPEGTPAAAEPGPLNGGTDHAEVAAPEPAPATPEAERAAATQVEKSG
                     A"
     gene            complement(588..839)
                     /locus_tag="VFR00_02745"
     CDS             complement(588..839)
                     /locus_tag="VFR00_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011472961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0043953 - protein transport by the Tat
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HEU0158199.1"
                     /translation="MGSLSIWHWLLFLAVALLMFGGSGKISSIMGDVAKGIKSFKKGM
                     AEDDEAQKDDAARKDAPPKSIDGTAKPAEPASVQANKVG"
     gene            complement(957..>1572)
                     /locus_tag="VFR00_02750"
     CDS             complement(957..>1572)
                     /locus_tag="VFR00_02750"
                     /inference="COORDINATES: protein motif:HMM:NF019998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HEU0158200.1"
                     /translation="MDEPFSGLDVQLRERLQEETLTLLRETRATCVIVTHAPAEAIRL
                     GDRIAVMRAGRLVQAGTAEELYRNPADLFVARLFSEINEIPYQVQEGALRTPIGAFAV
                     PGLNEGEAAILCIRRRAIRLGGVGAGLPGRLLRARFLGDLALLEVAVEGFERPLLTLV
                     REGEQPPVGQEVSVSVAPESVLVFSAEPGHADDAELIPVEQERP"
BASE COUNT          251 a          545 c          494 g          282 t
ORIGIN      
        1 gaggtcacgc tccgctcttt tcgacctgcg tcgccgccgc gcgctcggcc tcgggcgtgg
       61 ctggcgcagg ttcaggcgcc gcaacctcgg cgtgatcggt cccgccgttg aggggtcccg
      121 gttcggccgc agccggcgtg ccctcggggg gtgtcgccgg cggctcagcg gccggcgcgc
      181 cggcgcgatc ctcgagcgcc gcgctgacgc tctcctgggc ctgggcccag tccttctcca
      241 ccgattgctc ggcggcgcgc atgctttgct cggcttcgcg gcccaggctt tccacgtctt
      301 tcttgagatc ggcgaattcc gactcgcgga tggcctcgtc gaactgggcg cggaactcgg
      361 ccgcctggcg cttgatgatg ccgacgtact tgccgatcgt gcgcaggagc gcgggcagat
      421 ccttcgggcc aatgacgagg agcgccacga taccgaggat caagagtttg ctggaggtga
      481 tgtcgaacat cggctcaacc taacggcatg ggagcgcgag gcaaactctc aaaagcttac
      541 gccgaggcct tggcggtgcc tgctgcgatc gaccaaacgt ccccgcttca gccgaccttg
      601 ttggcctgca cactggcggg ttcggccggc ttggcggtgc cgtcgatcga cttgggcggt
      661 gcgtccttgc gagcggcatc gtccttttgc gcctcgtcgt cctccgccat gccctttttg
      721 aagcttttga tgcccttggc aacgtccccc atgatcgagg atatcttgcc gctgcccccg
      781 aacatcaata gggcaacggc cagaaacagc agccaatgcc aaatactcaa gctacccatt
      841 cgcttgctcc tctcgcagcc acgctgcata cgcttggggt ttgccgcact atcgcagaaa
      901 gggcccgttg gggcaaggtt gccacactgc ttgtatctgc gcctatgcca ttgaaatcag
      961 ggtctttcct gctcgactgg gattaattcg gcgtcatctg catggcccgg ctcggcagaa
     1021 aagacgagca cgctttcggg cgcgacactg acggagactt cctggcccac cggcggctgc
     1081 tcgccctcgc gcacgagggt gagcaacggt cgctcgaagc cctccaccgc cacctccagc
     1141 agcgccaagt cgccgagaaa gcgcgcccgc aacaaccgcc ccggcagccc ggcgccgacg
     1201 ccgccgagcc ggatggcgcg ccgccggatg cacaaaattg ccgcttcgcc ttcgttgagg
     1261 cccggcacgg caaaggcacc gatcggcgtg cgcagcgccc cctcctgcac ctggtaggga
     1321 atttcgttga tctcggagaa gagccgcgcc acgaacagat cggccggatt gcggtaaagc
     1381 tcttcggcgg tgcccgcctg caccagacga cccgcgcgca tgaccgcgat gcgatcgccg
     1441 aggcggatcg cctcggcggg ggcgtgcgtc acgatcacac aggtggcccg ggtctcccgc
     1501 aacagcgtca aggtctcttc ctgcaggcgc tcgcgcagct gcacatcgag acccgaaaac
     1561 ggctcatcca tc
//
LOCUS       DASTMT010000129         2869 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_4630494, whole genome shotgun sequence.
ACCESSION   DASTMT010000129 DASTMT010000000
VERSION     DASTMT010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2869)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2869)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2869
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4630494"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            251..616
                     /locus_tag="VFR00_02755"
     CDS             251..616
                     /locus_tag="VFR00_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007610790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEU0158201.1"
                     /translation="MAESQHHLAALRELNRSFVRAVDAADVAWFDANLATDFYNTNPD
                     GSFVDRAAFLAQIGRGSSVKDIREHDVMIRILGDFAIIHARTRYQKPDGSQGEGRYTD
                     DWHWRDGRWQCVSAHVTRL"
     gene            complement(747..2375)
                     /locus_tag="VFR00_02760"
     CDS             complement(747..2375)
                     /locus_tag="VFR00_02760"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEU0158202.1"
                     /translation="MARYIFITGGVVSSLGKGLASAALGALLQARGYSVRLRKLDPYL
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFTGVPARKSDNITTGRLYQDILAKE
                     RRGDFLGGTVQVIPHVTDAIKQFVLSGNDGIDFVLVEIGGTVGDIEGLPFFEAIRQLG
                     NELPRGGSVFIHLTLLPFISSAGELKTKPTQHSVKELRSIGIQPDILLCRCDREIPRS
                     ERRKIALFCNVREAAVIPALDVASIYEVPLAYHREGLDGEVLAAFGIKDAPLPQLSKW
                     QTIADAIANPEGEVTIAIVGKYTGLKDAYKSLNEALFHGGIANRVKVRLEWIESEVFE
                     REDPAPYLENVHGILVPGGFGERGSEGKILAARFARQRRVPYFGICFGMQMAVIEAAR
                     DLVGVKGAGSTEFGPTPEPVVGLMTEWMRGNELEKRAAHGDLGGTMRLGAYTAKLAKG
                     SRVAKIYGGTEISERHRHRYEVNTRYRERLEEAGVRFCGMSPDGLLPEIIELPHHPWF
                     IGVQFHPELKSRPFEPHPLFASFIQAAVEQSRLV"
     gene            complement(2533..>2869)
                     /locus_tag="VFR00_02765"
     CDS             complement(2533..>2869)
                     /locus_tag="VFR00_02765"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEU0158203.1"
                     /translation="ATCLKGPGPHQRPVIEFLAEVFRQQTLAEAVAWLAGLDVCFGPV
                     NTLPEAFADANVAARGMLLTDELGRRHLAPVIRFADEPARPVLSEPALGADTEAVLDR
                     LRRERPRQG"
BASE COUNT          495 a          973 c          927 g          474 t
ORIGIN      
        1 taagggttaa ataatcgccg cagcctgcgc gggcatgttt aatgggctct tgcactattt
       61 ggccgcggca ccgggaagcc cctcccgccg ctggttgacc gagcccgggg cacggcgacc
      121 tgcgcggcgg caaggcgtgc cgccgccacg ccggaacgag gcgcgcacgc tctgccggcc
      181 ctccaccccc cgtgctatcg tgcccgtcgc cacggctgcg gccggcggct cggccaagga
      241 gattgcaccc atggcagaaa gccaacacca cctcgccgcg ctgcgcgagc tcaaccgcag
      301 tttcgtgcgc gcggtcgatg cggccgatgt cgcctggttc gacgccaacc tcgcgacgga
      361 cttctacaat accaaccccg acggcagttt tgtcgatcgc gcggcttttc tggcccagat
      421 cggccgaggc tcgtcggtca aggacatccg cgaacacgac gtgatgatcc gcatcctcgg
      481 cgattttgcc atcatccacg cccgcacccg ctaccaaaaa cccgacggca gccagggcga
      541 gggccgctac accgacgatt ggcattggcg cgatgggcgt tggcaatgcg tgtcggcgca
      601 cgtgacacgc ctgtagatga agtcccggcg cggcgaccct gatcctgcca aaagcgacga
      661 ccgcggtcgc ggcgaaccct gatgccaggc agcgcaagcg cccttgcgtc tgcgcgcgct
      721 cgtacacaca cgcgcgcacg gcaggcttag accagcctgc tttgctccac ggccgcctgg
      781 atgaaggacg caaacagcgg gtggggctcg aacgggcgcg acttcagctc ggggtgaaat
      841 tgtacgccga tgaaccaggg atggtgcggc agctcgatga tttccggcaa aaggccgtcc
      901 ggcgacatcc cgcaaaatct gacccccgcc tcctccaggc gctcgcggta gcgcgtattg
      961 acctcgtagc ggtgacggtg gcgctcggaa atttccgtgc ccccgtagat cttggccacg
     1021 cgactgcctt tggcgagctt ggccgtgtag gcgccgagcc gcatggtgcc gccgagatcg
     1081 ccatgggcgg cccgcttctc cagctcgttg ccgcgcatcc actccgtcat cagcccgacc
     1141 accggctcgg gcgtcggccc gaactcggtg gagcccgcgc ctttgacgcc gacgaggtcg
     1201 cgcgccgcct cgatcacggc catttgcatg ccaaagcaga tgccgaagta gggcaccctg
     1261 cgttggcgcg caaagcgggc cgccagaatt ttcccctccg agccccgctc gccaaagccg
     1321 ccgggcacca gaatgccatg cacgttctcg aggtaggggg caggatcctc gcgctcgaac
     1381 acctcgcttt cgatccactc caaccttact ttcacgcgat tggcgatgcc gccgtggaac
     1441 agcgcctcgt tcagcgactt gtaagcgtcc ttcagccccg tgtacttgcc gacgatggca
     1501 atcgtcacct caccctcggg attggcgatg gcatcggcga tggtttgcca cttggagagc
     1561 tgcggcaggg gcgcatcctt gatcccgaac gcggccagca cttccccatc gaggccctct
     1621 cggtggtagg cgaggggcac ctcgtagatg gaggcgacgt ccagggcggg gatcaccgct
     1681 gcctcgcgca cattgcagaa gagcgcgatc ttgcgccgct ccgagcgcgg gatctcgcga
     1741 tcgcagcggc agagcagaat gtcgggttgt atgccgatcg atctgagctc cttgacggaa
     1801 tgctgcgtcg gcttggtctt cagttcgccc gcgctcgaaa tgaaggggag cagggtcaag
     1861 tggataaaga cggacccgcc ccgcggcaat tcgttgccga gctggcggat ggcctcgaag
     1921 aagggcagcc cctcgatgtc gccgacggtg ccgccgattt cgaccaggac gaaatcgatg
     1981 ccgtcgttgc cgctcagcac aaactgcttg atggcgtcgg tcacgtgcgg gatcacctgc
     2041 accgtgcccc cgagaaaatc cccccgccgc tccttggcca ggatgtcctg gtagagccgc
     2101 ccggtggtga tgttgtcgga cttgcgggcc ggcacgcccg tgaagcgctc gtagtggcca
     2161 aggtcgaggt cggtttcggc cccgtcgtcg gtgacgaaca cctcaccgtg ctggtaaggg
     2221 ctcatcgtgc cggggtcgac gttgagatag ggatcgagct ttctcagccg aaccgaatag
     2281 ccgcgtgcct gtaagagagc cccaagagcc gctgaagcca gccctttgcc gagcgaggag
     2341 accacgccgc cggtaatgaa gatgtaccgc gccatgggcc tttaacatac agcgacacgg
     2401 cacgattcgt agagtgtttt ttagcttcca tccacggcct ttcgccgcgc cggatgagac
     2461 gccggttgca gggcggatgc ggcgcagggg cggctcgtgc ccaagtccgg gggatccgag
     2521 cgccctgctc gcttagccct ggcgcggcct ttcccgccgc aggcgatcca acaccgcctc
     2581 ggtgtcggcg ccgagcgccg gctcggacag gacgggccgg gcgggctcgt cggcaaagcg
     2641 gatcaccggg gcgaggtggc gccggccaag ctcgtccgtc agcagcatgc cgcgcgccgc
     2701 cacattggca tccgcgaacg cctcgggcag cgtattcacc gggccgaagc agacatcgag
     2761 gcccgccaac caggcgaccg cctctgcgag cgtctgctgc cggaacacct cagccaaaaa
     2821 ttcgatcacc ggacgctggt ggggaccggg acccttgaga caggtcgcg
//
LOCUS       DASTMT010000130         5545 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_6270846, whole genome shotgun sequence.
ACCESSION   DASTMT010000130 DASTMT010000000
VERSION     DASTMT010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 5545)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5545)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5545
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6270846"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2734
                     /gene="dnaE"
                     /locus_tag="VFR00_02770"
     CDS             <1..2734
                     /gene="dnaE"
                     /locus_tag="VFR00_02770"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013948493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEU0158204.1"
                     /translation="SAQEMARLFADLPEALANSIEIAKRCAFRPLGRKPILPKFVVAA
                     KGASEAEQLALEAKELRAQAEAGLEQRLATTPLAPGFTAQDYAQRLAFEVDVISRMKY
                     PGYFLIVADFIKWAKANGIPVGPGRGSGAGSVVAWALTITDLDPLRFGLLFERFLNPE
                     RVSMPDFDIDFCQDRRDEVIRYVQGKYGADRVAQIITHGKLQARAVLRDVGRVLQMPY
                     GQVDRLCKLVPNNPANPVSLAQAIEIEPKLQEERDREPVTARLLEIAQKLEGLYRHAS
                     THAAGMVIGDRPLQELVPLYRDPKSSFPITQFNWKLVEAAGLVKFDFLGLKTLTVLRK
                     AVDLVKRGRGIDIDLATLPLDDGPSYALLARADTVGVFQLEGTGMRDSLKRLKPDRFE
                     DIIAMVALYRPGPMDNIPTYINRKHGEEPVELLHPRLEGILKETYGVIIYQEQVMQIA
                     QVLAGFSLGEADLLRRAMGKKDKREMAQQKSRFVEGALKGGVKRADAEYIFELVDKFA
                     GYGFNKSHAAAYALVSYQTAYMKANFREEFLAASMTLDMGNTDKLAMYTAEANKATIA
                     ILPPCINASEVDFLAGVGSIRYSLAALKNIGAQAVESIVGERRAGGQFRDLSDFARRC
                     NTKALNKRALETLSAAGAFDALEANRALVHGNVEQLLAFANRLAANEAQGTGDLFGGG
                     GAGEAAIDMRPTETWTPMERLQQEFEAIGFFLSGHPLDAYASALPKLGVSSYAEFEAR
                     CERGAAAGRIAGIVVSVRERRSQKGNKFAFAIFSEPTGQFEAVIFSETLAAARPLLEA
                     GTAVLLTVEGERDGEALKLRAQAVQSLDAAAASVQKGWKVVLDADRLSKHGCRDLAQL
                     GERLKPGGKGEIALSFLVQGREVSITFPQRRFDVSPAQKGAIATLPGVLEVVDI"
     gene            complement(2774..3361)
                     /locus_tag="VFR00_02775"
     CDS             complement(2774..3361)
                     /locus_tag="VFR00_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008834425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2585 family protein"
                     /protein_id="HEU0158205.1"
                     /translation="MPNSLTLPRLTLPAALALCAAIMLLTAALLAWMGHVVICKCGTI
                     KLWHASRTDSETSQHLTDWYTYSHILHGIILYWLTGILARGRLSVAARLVIAVSIEAA
                     WEVFENTPYIINRYRSVTISRDYFGDSIVNSMADIGAMILGFLLAARLPAWVTVALLI
                     AVEVTLLVLIRDNLILNAIMLIHPTEWLRTWQAGG"
     gene            3534..4715
                     /locus_tag="VFR00_02780"
     CDS             3534..4715
                     /locus_tag="VFR00_02780"
                     /inference="COORDINATES: protein motif:HMM:NF018302.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1134 domain-containing protein"
                     /protein_id="HEU0158206.1"
                     /translation="MRHVILLAMVLGLGGFSAAEAAQCSKDDFGKAVDQAGAALRKLN
                     ADNAPRLRAKMRELKERKAWSDADFEDKAMAAVQDERIEAYNAQANDLLAKLDTLGSV
                     EPTSEADCSKIGELSAASLELQATIKAKTAYTLSKLDQMLAPPAPAAPEPKTKAEKPA
                     EAAKARSEPVKPAPAPAPSAAPKSAAAGANKAPETSAWSAQTKGGQPRDVAVLEPSPQ
                     VSLAPLPPANPAPDEDGYSIDEIMAASAGFFGKVSANLGAVIEYLFQKSGRPTGYVLG
                     TEGGGALLAGVRYGSGTLYLRAGGSQKIYWHGPSLGTDVGAEGSKTLFLIYRLGSPDQ
                     MYASFTGIDGSAYLVGGVGATFVTNGNVIMAPIRSGMGLRLGANIGYIRFTPKETWNP
                     F"
     gene            complement(4728..5069)
                     /locus_tag="VFR00_02785"
     CDS             complement(4728..5069)
                     /locus_tag="VFR00_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0158207.1"
                     /translation="MKGVAAAICVGALSLGLVPAGTAAQEGMGLAGIHDWVKVGGRTC
                     MADHFHDGQGTGPTKAQAQAAAIRAWVDFTAWEYGRRWASWQASVSKSGSCSGARGNI
                     SCNVSSRPCAR"
     gene            complement(5261..>5545)
                     /locus_tag="VFR00_02790"
     CDS             complement(5261..>5545)
                     /locus_tag="VFR00_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEU0158208.1"
                     /translation="VFTAGIVADDVKQDAKGQTQQILKEIDRLLKLGGTDKSKLVSAT
                     IWVTDIRNRDAMNEAWNAWTGGENLPGRACIEAALADPRMLVEIAVVAAK"
BASE COUNT         1013 a         1798 c         1796 g          938 t
ORIGIN      
        1 aagcgcgcaa gagatggccc ggctgttcgc agatctgccg gaggcgctcg ccaacagcat
       61 cgagatcgcc aagcgttgtg cctttcgccc gctggggcgt aagccaattc tgcccaagtt
      121 cgtggtcgcg gcgaagggag cctccgaggc cgagcagctg gcgcttgagg ccaaagagct
      181 cagagctcag gcggaggccg gtctcgagca gcggctcgcc accacgccgc tggcgccggg
      241 gttcacggcg caagactatg cgcagcggct ggccttcgag gtcgatgtga tctcgaggat
      301 gaagtatccc ggctactttc tgatcgtcgc cgactttatc aaatgggcca aagccaacgg
      361 cattcccgta ggtccgggac gcggctccgg cgccggctcg gtggtggcct gggcgctcac
      421 catcaccgac ctcgacccct tgcgcttcgg cctcctgttc gagcgcttcc tcaacccgga
      481 acgcgtatcg atgccggact tcgacatcga cttttgccag gatcggcgcg acgaggtgat
      541 ccgctacgtt cagggcaaat acggggccga ccgggtggcg cagatcatta cccacggcaa
      601 gctgcaggcg cgcgcggtgc tgcgcgatgt cggccgcgtg ctgcagatgc cctacggtca
      661 ggtcgatcgg ctttgcaagc tcgttcccaa caatcccgcc aatcccgtga gcctggcgca
      721 ggccatcgag atcgagccca aactgcagga ggagcgggat cgcgaacccg tcaccgccag
      781 gctgttggaa atcgcccaga agctcgaagg cctctaccgg cacgcgtcca cgcatgccgc
      841 gggcatggtg atcggcgacc gcccgctgca ggaactggtc ccgctctatc gcgatcccaa
      901 atcgagcttt cccatcaccc agttcaactg gaagctggtc gaggctgcgg gcctcgtgaa
      961 gttcgatttt ctggggctga agacgctcac cgtgttgcgc aaggccgtcg acctggtcaa
     1021 gcgcgggcgc ggcatcgaca tcgaccttgc gacgctgccc ctcgatgatg ggccgagcta
     1081 cgcactgttg gcccgggcag acaccgtcgg ggtgttccag ctcgaaggca cgggcatgcg
     1141 cgatagcctc aagcgcctga aacccgaccg cttcgaggac atcattgcca tggtggcgct
     1201 ctatcggccg ggtccgatgg acaacattcc aacctacatc aatcgcaagc acggcgagga
     1261 gccggtggag ctcctgcacc cgcgcctcga aggcattctg aaagagacct acggcgtcat
     1321 catctaccag gagcaggtga tgcaaatcgc ccaggtcctg gccggcttct cgctgggcga
     1381 ggctgacctg ctgcgccgcg ccatgggcaa gaaggacaag cgcgaaatgg cgcagcagaa
     1441 gagccgcttt gtcgaagggg ctctcaaggg cggcgtcaag cgcgcggatg cggagtacat
     1501 cttcgagctg gtcgacaagt tcgccggcta cggcttcaac aaaagccatg ccgccgccta
     1561 tgcgctcgtc agctatcaga cggcctacat gaaggccaac ttccgcgagg aatttctggc
     1621 cgcctccatg accctcgata tgggcaacac cgacaagctg gcgatgtaca cggccgaggc
     1681 gaacaaggcc accatcgcca tcctgccccc ctgcatcaac gcctccgagg tcgatttcct
     1741 ggccggcgtc ggctcgatcc gctactcgct ggcggcgctc aagaacatcg gtgcgcaggc
     1801 cgtcgaaagc atcgtcgggg aacggcgggc cggcggtcaa tttcgcgact tgtccgactt
     1861 tgcccgccgc tgcaacacca aggcgctcaa caagcgggcg ctggagactt tgagtgccgc
     1921 cggcgccttc gacgcgctcg aagcgaacag ggcgctggtg cacggcaatg tcgagcagct
     1981 cttggcgttc gccaatcggt tggccgccaa cgaggcccag gggaccggcg atctgtttgg
     2041 cggcggcggt gccggggagg cggccatcga catgcggccg accgaaacct ggacgccaat
     2101 ggaacgcctg caacaggaat tcgaggcgat cggcttcttc ctgtcgggcc accccctcga
     2161 tgcctacgcc agcgcgctgc ctaagctcgg ggtgtcgagc tatgccgaat tcgaagcgcg
     2221 ttgcgagcgc ggtgccgcgg ccggccggat cgccggcatc gtcgtgtccg tgcgcgagcg
     2281 ccgctcgcag aagggcaaca agtttgcctt cgccatcttt tccgagccga cggggcaatt
     2341 cgaggcggtg atcttctccg agaccctggc tgcggcgcgt ccgctgctgg aggcgggcac
     2401 ggcggtgctc ctgaccgttg agggcgagcg tgacggggag gccttgaagc tgcgcgcgca
     2461 ggcggtgcag tcgctcgatg cggcggccgc gagcgtgcag aaggggtgga aggtcgtctt
     2521 ggatgccgac cggctaagca agcatggctg tcgcgatctc gcccaattgg gggaaagact
     2581 gaagccggga ggcaaaggcg aaattgcgct gtccttcctg gttcaggggc gtgaggtcag
     2641 catcaccttt ccgcagcgcc gctttgatgt gtcgcccgcc cagaaggggg cgatcgccac
     2701 gctgcccggc gttctggagg tggtggacat ttagcgtcgg tgctcgggtc gacgggctcc
     2761 tgaccgtcgt ctctcaaccg cccgcctgcc atgtccgcaa ccactccgtc ggatggatca
     2821 gcatgatggc gttgaggatc aggttgtcgc ggatcaggac gagcagggtg acctcgacgg
     2881 cgatcagcag cgccaccgtg acccaggccg gcaagcgcgc cgccagcaaa aagccaagga
     2941 tcatggcgcc gatatcggcc atcgaattga cgatgctgtc gccgaagtag tcccgggaga
     3001 tcgtgacgct gcgatagcgg ttgataatat agggggtgtt ctcgaacacc tcccaagccg
     3061 cctcgatcga gacggcaatc accaaacggg cggcaacgga cagccgtccc cgcgccagga
     3121 tgcccgtcag ccaatagagg ataatgccgt gcaggatatg gctgtaggtg taccagtcgg
     3181 ttagatgttg ggaggtttcc gaatcggtgc gactggcatg ccaaagcttg atggtgccac
     3241 acttgcaaat gacgacgtga cccatccacg ccagcagcgc cgccgttaac agcatgatcg
     3301 cggcgcagag cgcgagcgct gccgggagcg tgagcctagg cagggtcagt gaatttggca
     3361 tggcctagct tcgcacgccc tcagcgtaag cggaacggta gctcaagttt gcggcatttt
     3421 aattggtgcc cgagcgctaa ggaggccaca gtcggggcgg gttcgtgcga gtcgggcctt
     3481 gttttgcccc aagtccgcac gagaaatggc caagcgatga gtgggacacg gccatgcggc
     3541 acgtgatttt attggcgatg gtactgggcc tcggcggctt ctccgcggct gaagcggccc
     3601 agtgcagcaa ggatgatttc ggcaaggccg tcgaccaagc cggcgccgcc ttgcgcaagc
     3661 tcaatgccga caacgcgcca cgcctgcgcg ccaagatgcg cgagctcaaa gagcgcaaag
     3721 cctggtccga cgccgatttc gaggacaagg ccatggccgc agtccaggac gagcgcatcg
     3781 aggcctataa cgcccaggcc aacgatctgc ttgccaagct cgacaccttg ggcagcgtcg
     3841 agcccaccag cgaggccgat tgcagcaaga tcggcgagct gagcgccgcc agcttggagc
     3901 tgcaggccac catcaaggcc aagacggctt atacgctttc caagctcgac cagatgctgg
     3961 ccccgcccgc ccccgcggcg cccgaaccca aaaccaaggc cgagaagccg gccgaggcgg
     4021 cgaaggcacg cagcgagccg gtaaaaccgg cgccagcccc cgcccctagt gccgcaccca
     4081 aatcggcggc cgcgggcgcc aacaaagccc ccgagaccag cgcctggtcc gcccagacca
     4141 agggcggtca gccccgcgac gtcgccgtgc tcgagccatc gccgcaggtc tcgttggcgc
     4201 cgctgccgcc ggccaacccg gcgcccgatg aagacggcta ttcgatcgac gaaatcatgg
     4261 cggcaagcgc cggcttcttc gggaaggtct cagccaacct gggggcggtc atcgagtatc
     4321 tgttccagaa gagcggccgt cccacgggct acgtcctggg caccgaaggc ggcggggccc
     4381 tgctggccgg cgtgcgctac ggctcgggaa cgctctacct gcgcgccggt ggcagccaga
     4441 aaatttattg gcacggcccc tcgcttggca ccgacgtcgg tgccgagggg tccaagacac
     4501 tttttctcat ctaccgtttg ggctcgccgg accagatgta tgccagtttt accggcatcg
     4561 acggttcggc ttacctggtg ggcggcgtcg gcgccacctt cgtcaccaat ggcaacgtca
     4621 tcatggcacc gatccgctcc ggcatggggc tgaggctcgg cgccaacatc ggctacatcc
     4681 gcttcacgcc gaaggagacg tggaatccgt tctaagccgc gggcgcctca acgggcgcag
     4741 ggtcgcgagg aaacgttgca gctgatgttg ccgcgcgcgc ccgagcagct gcctgacttt
     4801 gacacggagg cctgccagct cgcccagcgc cggccatact cccaggcggt aaagtccacc
     4861 caagcccgga tggcagccgc ctgcgcctgc gccttggtgg ggcctgtgcc ttgcccatcg
     4921 tggaagtgat cggccatgca ggtccgacca ccaactttga cccagtcgtg aatgccggcc
     4981 aagcccattc cctcctgggc cgccgtgccc gcgggcacaa gtcccaggct gagcgctccc
     5041 acgcaaattg ctgcagcaac ccctttcatg gctcccgccc ttctgatcat cgtgtgttcg
     5101 gctaagcctt aacgaacgct gagcgattcc gcttcggccg aaggtcggca ggcgctactt
     5161 tgcgcctgcg gcgctgcatt tgcgtcacac gtcagaggga cggtcttaaa aaagcttcct
     5221 ctataagtca ggaattggac gcctggcctc cgtcgctgtc ctatttggcc gcgacgacgg
     5281 caatctcgac cagcatgcgc ggatcggcca gcgcggcctc gatgcaggcg cgaccgggga
     5341 ggttctcgcc gccggtccag gcgttccagg cctcgttcat ggcatctcgg ttgcgaatgt
     5401 cggtgaccca gatggtggcg gagaccagct tggacttgtc cgtgccgccc agtttcaaga
     5461 gccggtcgat ctccttcagg atctgctggg tctgaccctt ggcgtcctgc ttgacgtcgt
     5521 cggcgacaat gccggccgtg aacac
//
LOCUS       DASTMT010000131         2663 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_4631271, whole genome shotgun sequence.
ACCESSION   DASTMT010000131 DASTMT010000000
VERSION     DASTMT010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2663
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4631271"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            47..718
                     /locus_tag="VFR00_02795"
     CDS             47..718
                     /locus_tag="VFR00_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008969192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEU0158209.1"
                     /translation="MLYSASTWVLPVIIAITLHEAAHGFAAYLLGDNTAWRLGRVSLN
                     PLKHIDPFGTLLLPAMLLFLRAPFLFGYAKPVPVNFSALNHPRRDMVLVAIAGPATNL
                     VLATLAALLFHLVAFFPTEAALWLVQNLRNALIINVVLAVFNMIPLPPLDGGRVAVGL
                     LPNVLALPLARLEPYGVMILLTLMLFAPILGAQMGVDLNIISRLIGGPTDAILGAILR
                     LTGNG"
     gene            839..1879
                     /locus_tag="VFR00_02800"
     CDS             839..1879
                     /locus_tag="VFR00_02800"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEU0158210.1"
                     /translation="MKFGLFGGAKSSGDGPVGDSIGYAKYIDYVLRAEALGFHSVFVV
                     EHHFTGVGQLSASLNFLSYLAGRTERLRLGTAVVVLPWHNPVLLAEQVATLDLLSHGR
                     LDFGVGKGYRDAEFAGFSIPVAEASARFDETMAFLRQAWAAKDRFSHHGHYWRFENVI
                     IEPRPVQLPHPPLWMGAGSLSSIERAARDGFNLLLDQIAPVELIIERVAAYREALNRA
                     GRPYRPGQIGVARALQIVRTDEERQQAQQLRIKVLKAIGGLARGRGAERYRNIGSHAE
                     ADLASEEAALLGTPEEIIARLKKLAAGGVDYVLLVEPTASVAALETFAEEIMPHFALP
                     SPAAVALEEERR"
     gene            1876..>2663
                     /locus_tag="VFR00_02805"
     CDS             1876..>2663
                     /locus_tag="VFR00_02805"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEU0158211.1"
                     /translation="MKHAVAFYSEGCRLSGDLYLPRDVRAGDRRAGIVLCHGYTGVKD
                     LYLPDNARILNDAGYVVLTFDYKGWGASEGPRSRLAPYGRVADVQAALTFLSLQPQVD
                     PGRLGIYGTSYGGATVVWVGAIDPRVKCIVSVVGIGHGQRWMRSVRRPDEWHDLLDKA
                     KADREKRVIDGSSAMADRAEILLPDRQSAELAAAARRANPAAVNELPLEFIDDTLGFH
                     PEWVVDKIAPRPILFITSDDDRLVPPEESEALFAKAGEPKKLVT"
BASE COUNT          421 a          919 c          804 g          519 t
ORIGIN      
        1 cgaccgctgc agccctatcg atcggcgagt ccccatgtcc cccgaaatgc tctattcggc
       61 ctccacctgg gtgctgccgg tgatcatcgc cattaccctg cacgaggctg cgcacggctt
      121 cgccgcctat ctgctcggag acaacacggc ctggcggctc gggcgggtga gcctaaatcc
      181 gctcaagcat atcgaccctt tcggcaccct gctgctaccg gcgatgcttc tctttctgcg
      241 cgcgcccttt ctgttcggct acgccaaacc ggtgccggtg aatttttcgg ccctcaacca
      301 tccccgccgc gacatggtgc tggtcgccat cgccggtccc gccaccaacc tcgtgctcgc
      361 caccctcgcc gccctgctct ttcacctcgt cgcctttttc cccaccgaag ccgcgttgtg
      421 gctggtgcaa aatttgcgca atgcgctgat catcaatgtg gtcctggcgg tgttcaacat
      481 gatcccgctg cctccgctcg acggcggccg ggtggcggtc ggccttttgc ccaatgtttt
      541 ggccttgccg ctggcccgat tagagcccta cggcgtcatg atccttctca cgctgatgtt
      601 gtttgcaccc atcctcggcg cgcaaatggg tgttgacctc aacattatct cccgcctcat
      661 cggcggcccg accgacgcca tcttgggtgc catcctgcgc ctgaccggca acggttgagc
      721 gcgaccccgc ggcacggcgg gctacctcgc aagcaagcct catggcattg tgggcgtcgg
      781 cctggtggcg gcctagcggc ggcctagcgg cggcaagtgc tcgcggcgga gatggtgcat
      841 gaaatttggc ctgtttgggg gagccaagtc gtccggtgac gggcctgtcg gcgatagcat
      901 cggctacgcc aagtacatcg actacgtgtt gcgcgccgag gcgttgggct ttcacagcgt
      961 gttcgtggtc gagcaccact tcactggcgt cggccagctc tccgcttcct tgaacttttt
     1021 gagctatctc gccggacgca ccgagcgcct gcgcctcggc acggccgtcg tggttttgcc
     1081 atggcacaac ccggtgctgt tggcggaaca ggtggcaacg ctcgatcttc tttcccacgg
     1141 acgcctcgat tttggcgtcg gcaagggcta ccgcgatgcc gaattcgccg gcttctcaat
     1201 tcccgtggca gaagccagcg cgcgctttga tgagaccatg gcgttcctgc gccaggcttg
     1261 ggccgccaag gatcgcttct cgcatcatgg ccactattgg cgttttgaaa acgtcatcat
     1321 cgaaccccgt ccggtccagc ttccccaccc gcccctgtgg atgggagcgg gcagccttag
     1381 ctcgattgaa cgggcggctc gggatggctt caacctgctg ctcgaccaga ttgcgccggt
     1441 ggaactcatc atcgaacggg tggccgccta tcgtgaggcc ctcaaccgcg cgggccgtcc
     1501 ctaccgcccc ggtcagattg gcgtggcccg cgccctacag atcgtgcgca ccgacgagga
     1561 gcggcagcag gcccagcaat tgcgcatcaa ggttctcaag gccatcggcg gcctggcgcg
     1621 cggacgcggc gccgagcgct accgcaacat cggctcgcat gccgaggccg accttgccag
     1681 cgaggaggcc gccttgctgg gcacgcccga agagatcatc gcgcgcctga aaaagctcgc
     1741 cgccggtggc gttgattacg tgttgctggt cgagccaacg gcctcggtgg cggcattgga
     1801 gaccttcgcc gaggagatca tgccgcattt tgcgttgccg tccccagccg cggttgccct
     1861 cgaggaggaa aggcgatgaa acacgccgtc gcattctaca gcgaaggctg cagactttcc
     1921 ggagacctct atctgccgcg cgatgtgcgc gccggcgacc ggcgcgccgg catcgtgctg
     1981 tgccacggct ataccggtgt caaggatctc tacctgccgg acaatgcccg catcctcaac
     2041 gacgcgggct atgtcgtgct caccttcgat tacaagggct ggggcgcaag cgagggcccg
     2101 cgtagccggc tggcgcccta cggccgggtc gccgacgtgc aagcggcgct gacctttttg
     2161 tcactgcagc cccaggtcga tcccggtcgg ctcggcatct atggcaccag ctacggcggc
     2221 gccaccgtcg tctgggtcgg cgccatcgac cctcgcgtca agtgcatcgt cagcgtcgtc
     2281 ggcattggtc acggccaacg ctggatgcgc agcgtgcgcc gccccgacga atggcacgat
     2341 cttctcgaca aggccaaggc cgaccgtgaa aagcgcgtca tcgacggcag ctcggcaatg
     2401 gccgaccgcg ccgagatcct gttacccgat cgtcaatccg ccgagcttgc cgccgccgcg
     2461 cgccgcgcca accccgccgc ggtcaacgaa ctgccgctcg agttcatcga cgataccttg
     2521 ggcttccatc ccgagtgggt cgtcgacaaa attgccccgc gcccaattct ctttatcact
     2581 agcgatgacg accgcctggt gccacctgag gaatccgaag cgctgtttgc caaggccggt
     2641 gaacccaaga aactcgtgac ctt
//
LOCUS       DASTMT010000132         1576 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_4960264, whole genome shotgun sequence.
ACCESSION   DASTMT010000132 DASTMT010000000
VERSION     DASTMT010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 1576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1576
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4960264"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            25..213
                     /locus_tag="VFR00_02810"
     CDS             25..213
                     /locus_tag="VFR00_02810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0158212.1"
                     /translation="MDADPFHLLVGGEASPELIQGVKERAKVIEDDWVKHASAKGLDG
                     GKVLAEFREEIKRVAAGN"
     gene            225..749
                     /locus_tag="VFR00_02815"
     CDS             225..749
                     /locus_tag="VFR00_02815"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HEU0158213.1"
                     /translation="MASPQFDAGSWLDRALGAAAALMLFALMALTAVDVVSRYLFNWP
                     LRGAFEITELLLLSLIFAGLPLASRAGEHVTLDFIDHALTAAGRRLLRRLLDLVCGVI
                     LLALAWRVWVKADKIAGYGDTTEVLRLPVSPFVYVMALMVAITAVVHLAKVVFPGPDD
                     RPEPTFDPDKISPT"
     gene            746..>1576
                     /locus_tag="VFR00_02820"
     CDS             746..>1576
                     /locus_tag="VFR00_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009465701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEU0158214.1"
                     /translation="MTEALIGLGLMLVLALLRVPIALAMGVMGFLGVAYLRDWNFAPA
                     MAMVETKIYETGRNYTLSVVPLFILMGNLVTRAGMSQELFRAAYTFIGHLRGGLAMAT
                     VVACAGFGAICGSSIATAATMAKVAYPAMKGLGYSDRLSTGAIAGGGTLGILIPPSTL
                     MVIYGVLTETNIGKLFAAGVLPGIVATALLCLAVQWTVFVDPASGPHGERSSWAERWR
                     ALKGVWGVLALFIVVMGGIYGGVFTATEGAGMGAFGALLFALARRSLSWRMLLDALVE
                     S"
BASE COUNT          219 a          508 c          516 g          333 t
ORIGIN      
        1 taacgggttg ctcgtgacgg tgatttggat gccgaccctt ttcatctcct cgtaggcggc
       61 gaggcctcgc ccgagctgat ccaaggcgtc aaggagcgcg ccaaggtcat cgaggatgac
      121 tgggtcaagc atgccagcgc caaaggcctc gatggcggca aggtactggc cgagttccgc
      181 gaagagatca aacgcgttgc ggctggcaat tgacggggcc gacggtggct tcgcctcagt
      241 tcgatgccgg ctcctggctc gatcgggcgc tgggggcggc ggcagcactc atgctgtttg
      301 ccttgatggc gctgacggcg gtcgacgtcg tcagccgcta cctgttcaat tggccgctgc
      361 gcggcgcctt tgagattacc gagctcctgc tgctgtcgct aatctttgcc ggactgccgc
      421 ttgcctcacg cgccggcgaa cacgtgacgc tcgactttat cgatcacgcc ttgacggcgg
      481 caggacgccg gcttttgcgc cggctgctgg acctggtgtg cggcgtcatt ctgctcgccc
      541 tcgcctggcg tgtgtgggtc aaggccgaca agatcgccgg ctacggcgac accacggagg
      601 tgctgcgcct accggtcagc ccattcgtct acgttatggc gctgatggtc gccatcacgg
      661 ccgtggtgca tctcgccaag gttgtgtttc cgggtcccga cgatcgcccc gagccgacgt
      721 tcgatccaga caaaatcagc cccacatgac ggaagccctg atcggtctcg gcctgatgct
      781 cgtgctcgcc ctgttgcgcg ttccgatcgc gctcgccatg ggcgtgatgg gctttctcgg
      841 cgtcgcctat ctgcgcgatt ggaactttgc gccggccatg gcgatggtgg agaccaagat
      901 ctacgagacc gggcgcaact atacgctttc ggtggtgccg ctcttcattc tgatgggcaa
      961 tctggtgacg cgggccggca tgtcgcaaga gctttttcgc gccgcctaca ccttcatcgg
     1021 ccacctgcgc ggcggcttgg cgatggcaac ggtggtggcc tgcgccggct tcggtgccat
     1081 ttgcggctcc tcgattgcca ccgccgccac catggccaag gtcgcctacc cggccatgaa
     1141 ggggctcggc tactcggatc gcttgtcgac gggtgccatc gccggcggcg gcaccctcgg
     1201 catccttatt ccgccctcga cgctgatggt gatttacggc gtcttgaccg aaaccaacat
     1261 cggcaagcta ttcgccgccg gcgtgctgcc tggtatcgtc gccaccgcgt tattgtgcct
     1321 ggcggtgcaa tggaccgtct tcgttgaccc ggcctccggc ccccacggtg agcgctcgag
     1381 ctgggcggag cgctggcgcg cgctcaaggg cgtgtggggg gtcttggccc tcttcatcgt
     1441 cgtcatgggc ggcatttacg gaggcgtgtt cacggccacg gaaggcgcag gcatgggcgc
     1501 cttcggtgcg ctcctctttg ccttggcccg ccgcagcctg agctggcgca tgctgctcga
     1561 tgcgctcgtt gaatcc
//
LOCUS       DASTMT010000133         2850 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_1384091, whole genome shotgun sequence.
ACCESSION   DASTMT010000133 DASTMT010000000
VERSION     DASTMT010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2850)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2850)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2850
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1384091"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(346..1935)
                     /locus_tag="VFR00_02825"
     CDS             complement(346..1935)
                     /locus_tag="VFR00_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEU0158215.1"
                     /translation="MPHSPTRSIGPERARAKDARAAVAEVIRDIRARLLQGTVLRPEF
                     EYELLAMFVRNELSARVTVPLLAVIFSLASMFWAPVLQASAWLALVISAKFFMIAACR
                     KFLAQPRGTVRVDEWRRRIVLLELASGVTWGGMAVVGFGTGDTASHVFMLTSLIVLLA
                     IRMTFASTLMTVLYVGTTPMTVAVVVRLILQGHPFYYAMAAMAIGLHVYFLFLAKGLN
                     STALSMLEFRAEKDALIAEIEEEKWISDEARQRAEAANVAKSRFLATMSHELRTPLNA
                     ILGFSEVMKSELLGPMANANYKEYCANIHDSGRHLLQVINEILDLSRIEAGRYELQEE
                     PVRLSDVVEDCLRLLSLRAESKGLFVSPEFARGLDQLWADERAVRQICLNLLSNALKF
                     TPRGGRITLSVCSNRDGGQTLIVKDTGPGIPKEEIPRVLQAFGQGSLAHETAEGGTGL
                     GLPIVQNLIRLHGGTFELRSELRKGTEAIVAFPKSRVLKSMPPLQPLGQERHRQASAF
                     LAGARRAVGEAVRREDSTMLR"
     gene            2171..2782
                     /locus_tag="VFR00_02830"
     CDS             2171..2782
                     /locus_tag="VFR00_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007008174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HEU0158216.1"
                     /translation="MKLIQTKAAPNPRRVRIFLAEKGIEVPYQEVDLMQGALKAPEFT
                     ALNPFQRVPVLVLDDGTAIAETMAICRYFEELTPEPRLFGSGPVGVATVEMWNRRMDL
                     GLLLSVAQAFRHLHPAMAKLEVPQVAAWGDANKPRALEILQIMDDQLASRRFLAGEHY
                     SVADITALVAVEFMKPARIERPQGLKHLARWYEDVASRPSAKA"
BASE COUNT          516 a          894 c          964 g          476 t
ORIGIN      
        1 agagagcaag cttggatgct ccgcgcgcca taccgccttg ggcttttcgg gcatcaatgt
       61 aggaagcata agccgggctg acaatctcga ggcgccgggc ctgcggcggc ggctaggcag
      121 cgggccggac ccggcgcgcc ggggagcggc ggcgctgagg ctgggcagcg ccgagggcgc
      181 cccctgggga gcgggctggt gcgggcaagc ggggcccacc cgccgtgacg gcggcccgac
      241 gcgggagcca agcgaacctc caggcaagaa gccctccatg gctggggccg cgcgccggca
      301 tggcctctct tggcctggcg cgacacgagc cccgctcgac cgcttttagc gcagcattgt
      361 ggagtcttcg cgcctaacgg cctccccgac cgcacgtcgt gcccctgcca ggaaggcgga
      421 cgcctgacgg tggcgctcct ggcccaaggg ttgcaggggc ggcatcgact tgagcacccg
      481 gcttttcggg aaggcgacga tggcctcggt gcctttgcgc agttcggagc gcaattcgaa
      541 ggtgccgccg tgcaggcgaa taaggttctg cacgatcggc aggccgaggc cggtgccgcc
      601 ttcggcggtc tcgtgcgcca gcgagccctg tccgaaggcc tgcagcacgc gcgggatctc
      661 ctccttggga atgcccgggc cggtgtcttt gacgatcagt gtctggccgc catcgcggtt
      721 gctgcaaacg ctcaaggtga tgcggccgcc gcgcggggtg aatttgaggg cattggacaa
      781 gagattgaga cagatctggc gcaccgcgcg ttcgtcggcc caaagctggt cgagcccacg
      841 cgcgaactcg ggactgacga acagtccttt gctctctgca cgcaagctca acaggcgcag
      901 acagtcctcc accacgtcgg agaggcgaac gggttcttcc tgcagctcat agcgtccggc
      961 ttcgatccga gacagatcga gtatttcgtt gatcacctgc aggagatggc ggccgctatc
     1021 gtggatgttg gcgcagtatt ccttgtagtt tgcgttggcc atcggaccca gcaactcgct
     1081 cttcatcact tcggagaacc caaggatggc attgagcggc gtgcgcagct cgtggctcat
     1141 ggtggcgaga aagcgcgatt tggcgacatt ggcggcctcg gcccgttgcc gcgcttcatc
     1201 ggagatccat ttctcctctt cgatctcggc gatcagagca tccttctcgg cgcgaaactc
     1261 cagcatcgag agggcggttg aattgaggcc cttggcgagg aacaggaagt aaacgtgcag
     1321 gccgatcgcc atggccgcca tggcatagta gaaggggtgg ccctgcagga tcagccgcac
     1381 cacgacggcc accgtcatcg gcgtggtgcc cacgtacaag accgtcatca gcgtcgatgc
     1441 gaaggtcatg cgaatggcca acagcacgat gagggaggtc agcatgaaga cgtgggaagc
     1501 cgtgtcgccg gtgccgaagc caacgacggc catgccgccc caggtgacgc cgctcgccag
     1561 ttcgagcaag acgatgcgcc ggcgccattc gtcgacccgc accgtgccgc gcggttgggc
     1621 aagaaacttg cggcaggccg cgatcatgaa aaacttggcg gagatgacga gcgccagcca
     1681 ggcggaggcc tgcagcaccg gcgcccagaa catcgaggcc aaagaaaaga tgaccgccaa
     1741 cagcggcacc gtcacgcgcg ccgaaagctc gttgcgcacg aacattgcca acagctcata
     1801 ctcgaactcg ggcctgagca cggtgccctg cagcaggcgc gcccggatgt cgcgtatgac
     1861 ctcggcgacg gccgcccgcg cgtccttggc gcgtgcccgc tcgggaccta tgctcctggt
     1921 cggtgaatgg ggcatcgtct ccctcgccat ctcggccgaa gcaaacatgg gaagcgacgc
     1981 gatgggtgcg tgcgcagcgt ggattgagga tcgcggcaaa accctaacgt cccgtttatc
     2041 aatgtattat ccgggcgggt tagtgtgaca taagagcgtt gcagcgacgg cggcggcggc
     2101 ccacgctttg cttgcacggg caactgccct cctctaggag ttgcacatca aggacgaggg
     2161 caggcgagcc atgaagctca tacaaactaa ggcggcgccc aatccgcgcc gggtacgtat
     2221 cttccttgcc gagaagggca tcgaggttcc ctaccaggag gtcgacctca tgcagggcgc
     2281 gctcaaggcg cccgagttca cggccctcaa cccgttccag cgcgtgcccg tgctggtgct
     2341 cgacgacggc acggccattg ccgagaccat ggcgatctgc cgctacttcg aggaactgac
     2401 gcccgagccg cggctgttcg gcagcgggcc ggttggcgtc gccaccgtcg agatgtggaa
     2461 ccgtcgcatg gatcttggcc tgctgttgag cgttgcacag gcatttcggc acctgcatcc
     2521 ggccatggcc aagctggagg tgccgcaggt ggcagcctgg ggcgatgcca ataagccgcg
     2581 ggcgctggag atcctgcaga tcatggacga tcagctcgcc agccggcgtt tcctggccgg
     2641 cgaacattat tccgtcgccg atattaccgc tttggtggcc gttgagttca tgaaaccggc
     2701 gcggatcgaa cgcccgcagg ggctcaagca tctcgccagg tggtatgagg atgtggcaag
     2761 ccggccgagc gccaaggcct gagccgacgg cgccgggatc gcgatcatga agctcactat
     2821 cgtcggctgc ggcgatgcgt tcggctccgg
//
LOCUS       DASTMT010000134         2414 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_4960563, whole genome shotgun sequence.
ACCESSION   DASTMT010000134 DASTMT010000000
VERSION     DASTMT010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2414)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2414)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2414
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4960563"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..703)
                     /locus_tag="VFR00_02835"
     CDS             complement(<1..703)
                     /locus_tag="VFR00_02835"
                     /inference="COORDINATES: protein motif:HMM:NF021472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2189 domain-containing protein"
                     /protein_id="HEU0158217.1"
                     /translation="MPAMDVAKQQTGARLAAARIALNEVAFDAPWAWLAAGWRDLWAA
                     PSISFAYGALFAGLAAALSVGLWRSGLESLILPLSGGFLLIGPVLAVALYETSRRREA
                     GAEVDLATVTRAAVGKAGQISFFGAILGFIYVVWLQLAFLLFMLFLGTNQLPPAKDFV
                     PMLLFQPFGLGLLVTGTCVGAALAFLVFAISAVSVPLLMTRQVDAVTAMATSVSAVRQ
                     NLKPMLLWAGLIAGFM"
     gene            complement(752..1351)
                     /locus_tag="VFR00_02840"
     CDS             complement(752..1351)
                     /locus_tag="VFR00_02840"
                     /inference="COORDINATES: protein motif:HMM:NF014573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEU0158218.1"
                     /translation="MQPQHCRRALRHIKARTRAPGDTELPPAIAKAQLAIGWAQHLLI
                     VHPLWIGSMPALLKAFFEQALRPGFALSPTPKGGWSKLLSGRRARVVVTMGMPASIYR
                     WYFGAPWLKSLTQNLGLVGVAPCRTTLIWQDRGDERAPAAQVVATPQRAGRPGRMRRQ
                     AMGSGPHGAWGTAAEAPGFAAFAPLEPAAHIDPDQGVRH"
     gene            1480..>2414
                     /locus_tag="VFR00_02845"
     CDS             1480..>2414
                     /locus_tag="VFR00_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013949637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEU0158219.1"
                     /translation="MTVRNFDSLLNPASVALIGASPKAGSVGCIVAQNLRRGGFAGPI
                     WFVNPKYRHLGEAPCFATVAALPAAPDLAVLVTPPDTVPPLIAELGAKGTRAAVVITA
                     GLRGALKQAMLEAARPHTLRVQGPNCVGLMLPRIGLNASFSLAAPAGDIAFLSQSGAL
                     ITGIVDWARGRSIGFSHVVSLGDMADVDFGDLLDYLAADPQARAILIYMEAITHAPKF
                     MSAARRAGRSKPVVVVKAGRSSAGAKAAQSHTGALAGSDAAYEAAFRRAGLLRVEELD
                     DLFNAAEILSRHPRLAGDRLAILTNGGGAGVLAADR"
BASE COUNT          426 a          807 c          785 g          396 t
ORIGIN      
        1 tcatgaagcc ggcgatcagg ccggcccaaa gcaacatcgg tttgaggttt tgacgtacgg
       61 cagacacgct ggtcgccatg gcggtgacgg catcgacctg tcgcgtcatc aggagtggca
      121 ccgatactgc cgaaatggcg aacaccaaga aggcgagcgc ggcacccaca caggtccccg
      181 ttaccaacag gcccaagcca aagggctgaa acagcagcat gggtacaaag tccttggccg
      241 gcggcagctg gttggtaccg agaaagagca tgaacaacag gaaggcgagc tgcagccaca
      301 ccacgtaaat aaagccgagg atggcgccga aaaagctgat ctgcccggcc ttgcccacgg
      361 cggcgcgcgt aacggtggcg aggtcgacct cggcgccggc ctcgcgccgg cggctggtct
      421 catagagcgc aaccgccaga acggggccga tcagcaaaaa gccgccgctg agtgggagga
      481 tcaaggactc aaggccgctg cgccacagcc ccaccgacag cgcggccgcg aggcccgcga
      541 acagtgcgcc gtaggcgaaa cttatgctgg gcgcagccca aaggtcgcgc catccggctg
      601 ccaaccaagc ccagggcgca tcgaatgcca cctcgttgag ggcgatccgg gctgctgcca
      661 ggcgggcgcc ggtctgttgt tttgccacgt ccattgccgg catcagggcc tccgccacaa
      721 aaaacgggtg tgtggcaaca attcacccgg ctcaatgccg tactccttga tcaggatcaa
      781 tgtgcgctgc cggctcgagg ggcgcgaagg ccgcaaaccc aggggcttcg gcggcggtgc
      841 cccaggctcc atgcgggccc gagcccatag cctgccgcct catgcggcct gggcgcccag
      901 cgcgttgagg cgttgcaacc acttgagccg ctggcgctcg ctcatcccct cgatcctgcc
      961 aaatcagcgt cgttcggcag ggcgccaccc cgaccaaacc caaattttgc gtcaagcttt
     1021 tgagccaggg cgctccgaag taccagcggt aaatgctggc cggcatgccc atggtcacca
     1081 cgacgcgggc gcgccgtccg gacaggagct tgctccagcc ccctttgggg gtcggcgaca
     1141 gggcaaagcc cgggcgcagc gcctgctcga aaaaagcctt cagcagcgcc ggcatcgagc
     1201 cgatccagag gggatggaca atgaggagat gctgggccca gccgatcgcc agctgggctt
     1261 tggcgatcgc cggcggcagc tcggtgtcgc ctggcgcgcg cgtgcgcgcc ttgatatggc
     1321 gcaaggctcg gcggcaatgc tgcggctgca ctgcgcggat aggtcctcgc accccccacg
     1381 ctatggtctc cgcaaaaagc gcctcgggaa gtgctatcct ggctcagtaa cgaagatcgg
     1441 gtgtaaaggg gcgttggcaa gcccggctcg gcggctttca tgaccgttcg caactttgac
     1501 agcctgctca accccgcgtc ggtcgccttg atcggcgcta gccccaaggc cggcagcgtc
     1561 ggctgcatcg tggcgcagaa tttgcggcgc ggtggatttg ccggcccgat ttggttcgtc
     1621 aacccgaaat atcgccacct tggcgaggcg ccctgttttg ccaccgtggc ggcgctgccc
     1681 gccgcgcccg atctcgccgt gctggtgaca ccgccagaca cggtaccgcc cttgattgcc
     1741 gagctcggcg ccaagggcac gcgcgctgcc gtcgtcatca ccgcgggact gcgcggggcg
     1801 ctcaaacagg cgatgctcga ggcggcgcgt ccccacactt tgcgcgtgca aggtcccaat
     1861 tgcgtcgggc ttatgctgcc acgcatcggc ttgaacgcca gcttttcctt ggctgcgccc
     1921 gccggtgaca tcgccttcct ctcgcaatcg ggtgccctta ttaccggtat tgtcgactgg
     1981 gcgcgcggcc gcagcatcgg cttttcgcac gtggtctcgc ttggcgatat ggccgacgtc
     2041 gatttcggcg atctgctcga ttacctggcc gccgacccgc aggcgcgcgc cattctcatc
     2101 tatatggaag cgatcacgca tgctccgaag ttcatgtccg cggcccgccg cgccggtcga
     2161 tcgaaaccgg tcgtggtcgt caaagccggt cgcagctcgg ccggcgccaa ggcggcgcag
     2221 tcgcacacgg gagctttggc cggcagcgat gccgcttacg aagcggcgtt caggcgcgcg
     2281 ggtctcctgc gtgtcgagga actcgacgac ctcttcaatg ctgccgaaat cctatcccgg
     2341 catcctcggc ttgcggggga ccggctggcc attctcacca atggcggggg tgccggcgta
     2401 ttggcggccg accg
//
LOCUS       DASTMT010000135         2283 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_730058, whole genome shotgun sequence.
ACCESSION   DASTMT010000135 DASTMT010000000
VERSION     DASTMT010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2283)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2283
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_730058"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1182
                     /locus_tag="VFR00_02850"
     CDS             <1..1182
                     /locus_tag="VFR00_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006472104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEU0158220.1"
                     /translation="ALPGLSQVAGIAEPKELVPGGHGHGCGHNMLGSAALLAATAVKN
                     YLAAAGIKGRVRYYGCPAEEGGAAKVFMVRAGHFDDVDIAITWHPAAINRVDDAVSLA
                     NTRIDFSFKGRASHAAAAPHLGRSALDAVELMNVGVNYMREHMPADARIHYAMIDAGG
                     VAPNVVQAKATVRYAIRARDLRGMRALIARVRKIADGAALMTETEASLKVVSAVSNLL
                     GNAPLEQAMQAQFERLGPPPFDAADRKYAADIQASLSPEDIASAYARAGVPQRADNPL
                     CDFIVPFGTKGAAMIGSTDVGDVSWVVPTVQARVATHAIGTPGHSWQITAQGKSGQAK
                     KGMVHAAKVMAGVAVDALQDASLITRAKADHTARTTATPYDCPLPQDVLPPLQPRPAT
                     V"
     gene            complement(1240..2061)
                     /locus_tag="VFR00_02855"
     CDS             complement(1240..2061)
                     /locus_tag="VFR00_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011970553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU0158221.1"
                     /translation="MQASICTACGTQYPPSEQPAETCSICQDERQFVPVGGQGWTTLE
                     RLRVGHFNAYREHEPNLIGIGTQPVFAIGQRALLILTPAGNVLWDCISLLDAATITLV
                     KALGGLKAIAISHPHFYTTMAEWAQAFDAPVYLNAADRKWVMRPHSRLQFWDGPTHAL
                     APGITLIRCGGHFPGGSALHWADGAGGRGALLSADMATVNMDRKSFTFMRSYPNMIPL
                     SAAQVQAIAAAVEPFRFDRVYGHFFDRVIEGGAKEILSNSVARYLAALQGAYEAE"
BASE COUNT          399 a          744 c          775 g          365 t
ORIGIN      
        1 gcgttgcccg gcctgtcaca ggttgccggc atcgccgagc cgaaggagct ggtgccgggc
       61 ggccacggcc acggctgcgg gcacaacatg ctcggctcgg cggcactgct ggcggcgacg
      121 gcggtcaaga attatttggc cgcggcgggg atcaaggggc gggtgcgcta ctacggctgc
      181 ccggccgagg aaggcggcgc ggccaaggtc ttcatggtgc gcgccggtca tttcgacgac
      241 gtcgacatcg ccatcacctg gcatcccgcc gccatcaatc gggtggacga cgcggtcagc
      301 cttgccaaca cgcgtatcga tttcagtttc aaagggcgcg cctcccacgc ggccgccgca
      361 ccgcacctcg ggcgcagcgc gctagatgcc gtggagctga tgaacgttgg cgtcaactac
      421 atgcgcgagc atatgccagc cgatgcacgc attcattacg ccatgatcga tgccggcggc
      481 gtcgccccga acgtggtgca ggcaaaggcc acggtcaggt acgccattcg cgcgcgcgat
      541 ttgcgcggca tgcgcgcgct gatcgcacgt gtccgaaaaa tcgcggacgg cgcggcgctg
      601 atgactgaga cggaagcaag cctcaaggtc gtgagtgcgg tctccaactt gctcggcaac
      661 gcgccactcg agcaggccat gcaggcccag ttcgaacggc tggggccgcc gccgttcgac
      721 gccgccgatc gcaagtacgc cgcggacatc caggcgagcc tctcgccgga ggatatcgcc
      781 tcggcctatg cgcgcgccgg agtgccacaa cgtgcggaca atccgttgtg cgatttcatt
      841 gtgccattcg gcacgaaggg tgcggccatg atcggttcga ccgacgtggg agatgtgagc
      901 tgggtggtgc cgaccgtgca ggcgcgcgtt gcaacgcacg cgatcggcac gccgggacat
      961 tcctggcaga tcaccgctca gggcaaatcg ggccaggcca aaaagggtat ggtgcatgca
     1021 gccaaggtga tggccggcgt ggccgtggat gccctgcagg acgcaagctt gatcacgcgt
     1081 gccaaggccg accacacggc gcgcacgacc gccacgccct atgactgtcc cttgccgcag
     1141 gacgtgctgc cgcccctgca gccgcgcccg gcgacggtct gaccggcccc gcgctggcgg
     1201 gtggtgcccc gcctgctgcg gccggcgggc tcgcgcagct cattcggcct cataggcgcc
     1261 ctgcagggcc gccaggtagc gtgccacgga gttgctcagg atctctttgg cgccgccttc
     1321 gatcacgcgg tcgaaaaagt ggccatagac ccgatcgaag cggaacggct caaccgccgc
     1381 cgcaatcgcc tgcacctgcg ccgccgagag cgggatcatg ttggggtagc tgcgcatgaa
     1441 ggtgaaggac ttgcggtcca tgttcaccgt cgccatgtcg gcgctcaaca gggcgccacg
     1501 cccgccggcg ccatcggccc agtgcaacgc gctgccgccc gggaaatggc cgccgcagcg
     1561 gatcagcgtg atgcccggcg cgagcgcgtg cgtgggacca tcccagaatt gcaggcgcga
     1621 atggggccgc atgacccact tgcgatctgc ggcattgaga tagaccgggg catcgaaggc
     1681 ctgcgcccac tccgccatgg tcgtgtagaa gtgcgggtgg gatatggcga ttgccttgag
     1741 accaccgagc gccttcacca gggtgatggt ggctgcatcc agcaaggaaa tgcagtccca
     1801 cagcacattg cccgcaggcg tgaggattaa cagggcgcgc tgaccgatgg cgaaaaccgg
     1861 ctgcgtgcca atgccgatga gattcggctc gtgttcgcgg taagcattga agtgaccgac
     1921 ccggagccgt tccaaggtcg tccagccctg cccgccgacc ggcacgaatt gtcgctcgtc
     1981 ttggcaaatg ctgcaggtct ccgccggctg ctcgctcggc ggatactggg tgccgcaagc
     2041 cgtacagata gaagcctgca tgagccgatc ctcctcagct aaaacggtgc gtggtcgcaa
     2101 cgcgggagcg agagctcccg cgcgcgtcac gggcggagtt aagccccaaa agggcccgcg
     2161 ttcaaaccgg ccggctagtc cagcttgatg ttggcggctt ggatcagctc ggcgttggcc
     2221 ttgatatcct gcagcaggtg cgccgccatt tcctcgggtg tctgcaacgg caccacggca
     2281 ttg
//
LOCUS       DASTMT010000136         5530 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_1385206, whole genome shotgun sequence.
ACCESSION   DASTMT010000136 DASTMT010000000
VERSION     DASTMT010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 5530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5530)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5530
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1385206"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..211
                     /locus_tag="VFR00_02860"
     CDS             <1..211
                     /locus_tag="VFR00_02860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0158222.1"
                     /translation="FAEQADGTWKERTSGLVTHDSRGQRVEASYRRALAACRSHGVSG
                     GPNAAARTQSMAALRSYLVKHLLER"
     gene            complement(371..736)
                     /locus_tag="VFR00_02865"
     CDS             complement(371..736)
                     /locus_tag="VFR00_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003579869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEU0158223.1"
                     /translation="MRDAAKLAEDYIALWNETDPSRRMALLAATWTSDATYVDPLMRG
                     QGHREIAGLVAAAQAKFPDLRFALLRPADGFGDHVRFSWGLGPQGHEPIIKGTDFVRR
                     DGDLVRSVVGFLDQVPAGA"
     gene            990..1565
                     /locus_tag="VFR00_02870"
     CDS             990..1565
                     /locus_tag="VFR00_02870"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU0158224.1"
                     /translation="MAWGRNLLMRLFGRPKGLLGRLGGLMMARMNRDAAVEVIGLLGV
                     RSDDSLLEIGFGPGVAIQLLVERVPGVRIAGVDPSLEMLSQASARNAAALRSRRVDLR
                     HGVVDALPFAAQTFDKALAINSMQVWPDPAAGLSEIGRVLKPAGRIGLGFTANSGQPK
                     EGVAAALAAAGFVDSQIVERPRLFCAIATKP"
     gene            complement(1629..3596)
                     /locus_tag="VFR00_02875"
     CDS             complement(1629..3596)
                     /locus_tag="VFR00_02875"
                     /inference="COORDINATES: protein motif:HMM:NF040438.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase domain-containing protein"
                     /protein_id="HEU0158225.1"
                     /translation="MPLVRFEIASRVPYAEGRRFGAVGAYEQLDGTAHFAVDPSHPAN
                     ARICDLKLAPRTAAGLVEFTSDFSLLQPLEAAKANGRCIVELPNRGRRRVVALMNGAP
                     PDAAVGPQAHPGDGFLFARGYSVASIGWQWDVYPSPELLGLAAPSAIRGGDPIGGQTM
                     VEMRPNTRETTRLLADRVHRPLPAAAGAELNARLLVRNWEDGEDTLIPRARWRFARET
                     AAGVVEPSSEHVWLEGGFEPGRIYQLVYDTDRAPVAGLGLLAARDVAAFLRRPSSSTA
                     NVPGFKTLILYGISQTGRMQRHFLSLALNRCEDGSRAYDGLHVHIAGARRGAFNHRFA
                     QPSNQTTPLWGHVFPFADVVTSDPLTGRTGGLLDRLAAADDLPKIISTDSAAEYWRGD
                     AALAHIDVAARHDLPEHPLARRYLFAGAQHTPGYLGQTRTNPSTGTIARYPLNVIDYL
                     PLHRAALVNLDRWISDNIEPPPSRHPRLSDASAVNREEVLAAFARLPGFTPPQPQRLP
                     FIRTVDMGSDEETGVGRYPAQEGAFYPALVSGVDADGNEIAGIRLPDVSVPVGTHAGW
                     NPRDPSTGSPEQIVPMNGLTLWFAPDEATRAARGDPRKSLAERYRDEADYAAKVRAAA
                     VALAAERYLLEDDVEPVVAAAVARYRAALLQ"
     gene            3912..4973
                     /locus_tag="VFR00_02880"
     CDS             3912..4973
                     /locus_tag="VFR00_02880"
                     /inference="COORDINATES: protein motif:HMM:NF025381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HEU0158226.1"
                     /translation="MHEITRAAIEAVGPDQPSDWELRPENHSAALRDPVAAAPLVQLL
                     QDPDLKPIVQQYASANRQAVAAQASYKRCARLSAIGSFLAVVIASFMLLPRIGGLHDE
                     AVTIVALAQFLLLVLSFLASLWLAWRRPYTTWMRKRADAETARIRLFRHVTTATGASQ
                     AAGLPVLPLQLEYFRRFHLDIQRLYYGRRGEQHRRAVRRRFAWRCIALTFIIAAALPI
                     VWTVQGKDWIPTWLGQWFAHLPPKGEFAQRLFLGLGLIGGALQGLLAAYALMSYDERN
                     SARYGETKENLDDLAARPLAEARAAAAAGDASRVYAFYALVEEQVSSEHREWTAIRQL
                     VPDLSLDRLRELRLPMAVR"
     gene            complement(5013..>5530)
                     /locus_tag="VFR00_02885"
     CDS             complement(5013..>5530)
                     /locus_tag="VFR00_02885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0158227.1"
                     /translation="GRRQDRPRLEADAVLTPADFRSIALAVAEPVLRARKTGFVAARR
                     ARISEHIETRWDGIETTNTARPGDWIVTNLTPDRKLLRDRKGHVNTYVISGRRFAALY
                     ERTPGKTRFGTIFQPKSQVSAIPLLGGFDIKAPWGKRQTARAGYLVLNGTEVYGSNAT
                     TFAATYQVIAR"
BASE COUNT          891 a         1786 c         1911 g          942 t
ORIGIN      
        1 cttcgccgag caggcagacg gaacctggaa ggagcgcacg agcggtctcg tcacccacga
       61 cagccgtggc caacgggtcg aggccagcta taggcgggcg ctggccgcct gccgcagcca
      121 cggcgtaagc ggtgggccga atgcggcggc gaggacgcag agcatggccg cgctcaggag
      181 ctatctcgta aagcatctgc tcgagagata gcagggcgag gcgcgccgcc aatggagagg
      241 cgaggccttt gtggctgctt ccggtggtgc tgcgcgggga cgacgcgggg gatgccgcgt
      301 tccctggcga cgacggctgc gggggcaacc gccgatcaca gtcctcctgt cggcgcacgc
      361 cgacccaagc tcaggcaccg gcgggcactt ggtccagaaa gccaaccacc gacctgacaa
      421 ggtcgccatc gcgccgcacg aagtcggtgc cctttatgat gggctcgtgc ccctgcggcc
      481 ccagacccca ggagaaccgg acgtggtcgc cgaagccgtc cgcaggcctc agcaaggcga
      541 agcgcaagtc ggggaacttg gcctgtgcgg ccgcgaccag acctgcaatt tcgcggtgtc
      601 cttgtccccg catcaggggg tcaacatagg tcgcatcgct cgtccaggtc gcggccaaga
      661 gagccatccg tcgtgaggga tcggtttcgt tccaaagggc gatgtagtcc tcggcaagct
      721 ttgcggcatc acgcatggtt gacctccatt ccggctgagc ggttgcaagc atcgccattg
      781 tgcgctcggg cctctgccgc ggcgattacg ctggaggtaa tgcggcgccg ctgcgcaggg
      841 cgcggttgcc ctcgcgtcgg cggctccgcg gcaaaacggt gttgtcagaa ccgcgggctg
      901 ccgcgtttcc cctattgctc ggcgcagcgc cgccgcgcca aagcgagcgc gacgcttccg
      961 ctttgccgcc tgttgcggga gctgaaccga tggcttgggg acgcaatctg ctgatgcggc
     1021 tgttcggccg ccccaagggc ctgctgggac gtctgggtgg actgatgatg gcgcgcatga
     1081 accgcgatgc cgccgtcgag gtcattggac tgcttggcgt ccgttccgac gacagcctcc
     1141 tcgagatcgg tttcgggccc ggcgtcgcca ttcagctgct agtagagcgc gtgcctggcg
     1201 tccgcatcgc cggcgttgat ccgtcgctcg aaatgctcag ccaagcgtcg gcccgcaacg
     1261 cggcggcgct gcgcagccgg cgggttgatc tgcgtcacgg tgttgtcgac gcgctgccct
     1321 ttgccgccca aaccttcgac aaggcgctgg caatcaattc gatgcaagtg tggccggatc
     1381 cggccgcggg cctcagcgag atagggcgcg tgctcaaacc agccggcagg atcggcctcg
     1441 gcttcacggc caattccggt cagccgaagg agggtgttgc ggcagcgctc gccgcagctg
     1501 gatttgttga cagccaaatc gtggagcggc cgaggctgtt ttgcgcgatc gcgacaaagc
     1561 cgtgatcggt atggggggac atagcgccat cccgagaggg caagggcaaa gccttttttt
     1621 tgcgcgccct attgcaagag cgctgcccgg tagcgcgcaa cggccgccgc gacgacgggc
     1681 tcgacgtcgt cctcgagaag gtagcgctct gccgcgagcg caacggcggc cgcgcgcacc
     1741 ttggcggcgt agtcggcctc gtcgcgatag cgctcggcaa gggatttacg cggatcgccg
     1801 cgcgcggcgc gcgttgcctc gtccggcgcg aaccagaggg tgaggccgtt catcggcacg
     1861 atctgctcgg gcgatccggt gctgggatcg cgtgggttcc agccggcatg cgtgccgacc
     1921 ggtacgctga cgtcgggcag gcggatgccg gcaatttcgt tgccgtcggc atccacgccc
     1981 gaaaccaggg ccggatagaa ggcgccctcc tgcgccggat agcggccaac gcccgtctcc
     2041 tcatcgctgc ccatatccac ggtgcggatg aagggcaggc gctgcggctg cggcggcgtg
     2101 aacccgggca gccgtgcgaa ggcggcaagc acctcctcgc ggttgacggc gctcgcatcg
     2161 ctgagacggg gatgccggct cggcggcggc tcgatgttgt cactaatcca acgatcgaga
     2221 ttgacgagcg ccgcacggtg cagcggcagg tagtcgatga cgttgagtgg gtagcgcgcg
     2281 atcgtacccg tgctcgggtt ggtgcgcgtc tgcccaagat agcccggcgt gtgctgggcg
     2341 ccggcaaaca ggtatctgcg ggccagcgga tgctcgggga ggtcgtgccg ggccgccacg
     2401 tcgatatgcg ccagcgcggc gtcgccccgc cagtactccg cggcgctgtc ggtggagatg
     2461 atcttcggca ggtcgtctgc ggcggcgagc cggtcgagca gcccgcccgt gcgcccggtc
     2521 agcgggtcgg acgtcaccac gtcggcaaac ggaaaaacgt ggccccacaa gggtgtcgtc
     2581 tggtttgagg gctgtgcgaa gcgatgattg aaggcgccgc ggcgtgcgcc cgcgatgtgc
     2641 acgtgcaaac catcgtaggc acggctgccg tcctcacaac gattgagggc gaggctcaaa
     2701 aagtgccgct gcatccgccc cgtctgcgag atgccgtaga ggatgagggt tttgaagccc
     2761 gggacgtttg cggtgctgga ggagggccgg cgcaggaacg ccgccacgtc gcgcgctgca
     2821 agcaggccga ggccggccac cggtgcgcgg tcggtgtcgt agacaagctg atagatgcgc
     2881 cccggctcga agccgccctc gagccagacg tgctcgctcg agggctccac cacgcccgcg
     2941 gcggtttcgc gcgcgaagcg ccaccgcgcg cgcggaatga gcgtgtcctc accatcctcc
     3001 cagttgcgca cgaggagacg cgcattgagc tcagcaccgg ccgcggccgg cagcggccga
     3061 tgcacgcggt cggcgaggag ccgggtcgtt tcgcgggtat ttggccgcat ctcgaccatg
     3121 gtctggccgc cgatggggtc accaccccgt atggccgaag gtgcggcgag gcctaacagc
     3181 tccgggctcg ggtagacatc ccactgccag ccgatggagg ccacgctgta gccgcgcgcg
     3241 aacaggaaac catcacccgg atgcgcctgc ggccccacgg ctgcatcggg cggtgcccca
     3301 ttcatcaagg caacgacgcg gcgacggccg cggttgggca attcgacgat gcagcgaccg
     3361 tttgcctttg ccgcctcgag cggctgcagc agggaaaaat cgctggtaaa ttcgaccagc
     3421 cccgcggccg tgcgcggcgc cagtttgagg tcgcagatgc gggcatttgc cgggtggctg
     3481 ggatcgaccg cgaagtgcgc cgtgccgtcg agctgctcat aggcgcccac cgcaccgaag
     3541 cgccggccct cggcataggg gacacgcgag gcaatctcaa atcttacgag cggcatgggt
     3601 gggcccttcc agttgctaga cgctcgggga ctggcgcgat gatgcgggat tgggcctcga
     3661 cccacaagcg cccgtggcgg caaggctgcc cgaggcgcgg gacggcggcg cgctgagtgt
     3721 ctcagggagg caacagttgt ttgtgtcgcg cgccggggac gtagatctga catcctgcga
     3781 gctctaggct ccgtctcggg gggtaggacc atccagagcg cgatgcgctg cgcggctcga
     3841 ggtcgaccaa ccctcgagcc ggggcctcaa catcggcccg agggagcctt gcatttgcat
     3901 aaaggcatag catgcacgag ataacgaggg ctgcgatcga ggccgtcgga ccggaccagc
     3961 caagcgactg ggagctcagg cccgaaaacc atagcgctgc cttgagagac cccgttgccg
     4021 cggcgcccct cgtgcaactc ttgcaagatc cggatctaaa gccgattgtg cagcagtatg
     4081 cgtcggccaa tcgccaggcg gtggccgccc aagccagcta caagcgctgc gcgcgattgt
     4141 ctgcgattgg cagtttcctg gccgtcgtca ttgccagttt catgctgctg ccgcgcatcg
     4201 gcggcctgca cgacgaggcc gtcaccatcg ttgcgctggc gcagtttctg ctgctggtgc
     4261 tgtcttttct cgcatccctg tggctggcgt ggcgacggcc ctatacgacc tggatgcgca
     4321 agcgcgccga tgccgagacc gctcgcattc gcttgtttcg gcacgtgacg acggccactg
     4381 gcgcaagcca agccgcgggc ctcccggtcc tgccgctgca gcttgagtac ttccgccgct
     4441 ttcatctcga catccagcgg ctctattacg gacgacgcgg tgaacagcac cgtcgggcgg
     4501 tgcggcgccg ctttgcctgg cgctgcattg ccctgacctt catcattgcc gccgcgctac
     4561 caatcgtgtg gacggtccag ggaaaggact ggatccccac ctggctcggt cagtggttcg
     4621 cccacctgcc cccaaagggg gagttcgccc agcgtctgtt tcttggcttg gggctcattg
     4681 gcggggcact gcagggcttg ctcgccgcct atgctcttat gtcctatgac gagcgcaaca
     4741 gcgcccgcta tggcgaaacc aaggaaaacc tcgacgatct tgccgcgcgg ccgctggctg
     4801 aagcgcgcgc ggccgcggcc gccggcgacg cctcccgcgt gtatgccttc tatgccctgg
     4861 tcgaggaaca agtttcctcc gagcaccgcg agtggacggc cattcgtcag ctggtgcccg
     4921 atctttcctt ggaccgcctg cgcgagctca gattgccgat ggcggtccga taaagacggc
     4981 cggcaatcgg ccgcgaccca aacctgaggg gctcaacgag cgatgacctg gtaggttgcc
     5041 gcaaaggttg tcgcattgct gccgtagact tccgttccgt tcaaaacaag gtagccggcg
     5101 cgcgccgttt ggcgtttccc ccaaggcgcc ttgatgtcaa agcccccgag cagcgggatc
     5161 gctgagacct gggactttgg ctggaagatt gtgccaaagc gcgtcttgcc gggcgtgcgc
     5221 tcataaagtg ccgcgaaacg tctgccgctg atcacgtagg tattgacgtg acccttgcgg
     5281 tcccgcaaca gcttcctgtc gggggtcaaa ttggtcacga tccaatcgcc cggccgtgcc
     5341 gtgttggtgg tttcgatgcc gtcccagcgc gtctcgatgt gctcggatat cctggcgcgc
     5401 cgcgccgcaa cgaagccggt cttgcgggca cgtagcaccg gttcagcaac ggctagggca
     5461 atcgatcgaa agtcggctgg cgtcagcacg gcgtcggctt cgagcctcgg ccggtcttgt
     5521 cggcgacctt
//
LOCUS       DASTMT010000137         2303 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_75811, whole genome shotgun sequence.
ACCESSION   DASTMT010000137 DASTMT010000000
VERSION     DASTMT010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2303)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2303
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75811"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..266)
                     /locus_tag="VFR00_02890"
     CDS             complement(<1..266)
                     /locus_tag="VFR00_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HEU0158228.1"
                     /translation="MDRPLTVRTYLDFEKPIAELESKVAELSALSVEDDGSVSITEEV
                     TKLQQKAHQALVDTYAKLTPWQKTQVARHPDRPHCLDYVKALIE"
     gene            complement(397..1344)
                     /locus_tag="VFR00_02895"
     CDS             complement(397..1344)
                     /locus_tag="VFR00_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEU0158229.1"
                     /translation="MATTREILEGFLEMQAAERGAAANTVAAYRSDLTHFLDHLERRG
                     KAPAAADSRDIVAYQRYLAEAEMAPSTRARRLSAVRQLFKFLAAEGVIGDDPARRVVG
                     PRKSRTLPKILSVADVDRLIAAAGARAQAARGRDRLRALRLNALIEVLYATGLRVSEL
                     VSLPRSVLDGDGKVLIVKGKGGRERMVPLTPAAREALARYLNAGLDDPGLTPMVRTRW
                     LFASRSREGHLTRQRLAQELKELAEAADLDPGRVSPHVLRHAFASHLLDRGADLRSVQ
                     QLLGHADISTTQIYTHVLEERLKKLVFEHHPLAPSAARR"
     gene            complement(1377..1520)
                     /locus_tag="VFR00_02900"
     CDS             complement(1377..1520)
                     /locus_tag="VFR00_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0158230.1"
                     /translation="MPSLIRFLVVVGIIGAAVIGGLYVMAVFFEPSQRETATPLPGVK
                     VRR"
     gene            1704..>2303
                     /locus_tag="VFR00_02905"
     CDS             1704..>2303
                     /locus_tag="VFR00_02905"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018699194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEU0158231.1"
                     /translation="MMRFGYGGKKEEQIAQLAAKVRAGLGTRSVVLVGMPGCGKSAIG
                     RRLASRLEMPFVDADDEIELAAGKAITDIFKDHGEPYFRDGERKVIARILNSGCQVLA
                     TGGGAFMSAETQDNIRQAAVSVWLKAELSLLLRRVLKRNNRPLLERDPEGVMRSLVET
                     RYPIYATADITVESRDLPHDVMVIEIIEALAAHALPRQPV"
BASE COUNT          403 a          757 c          745 g          398 t
ORIGIN      
        1 tcctcgatca aggccttgac gtaatcgaga caatgcggcc ggtccgggtg gcgggcgacc
       61 tgcgtcttct gccacggcgt gagcttggca taggtatcga cgagggcctg gtgcgccttt
      121 tgctgtagtt tcgtcacctc ctcggtgatc gagacggaac cgtcgtcctc tacgctcagc
      181 gcgctgagtt ccgccacctt gctctcgagc tcggcgatcg gtttttcaaa atccaaatag
      241 gtgcgaacgg tgagcggacg atccattgct gacgaccgtt ggttcggcac ggcgaaacct
      301 gcttgcgccg gcgacatagg cctcccggca gctacgagtc aagttaacgg gcgccttgct
      361 gctggccgcc gggcctgcct gcggcgcggt cgccgcttag cgccgcgctg ccgacggcgc
      421 cagcggatgg tgctcaaaaa ccagcttctt cagccgctcc tcgaggacgt gggtgtagat
      481 ctgcgtcgtc gaaatgtcgg catggcccaa cagctgctgt accgagcgca ggtcggcgcc
      541 ccgatccaag aggtgactgg caaaggcgtg acgcagtacg tgcggcgaca cccgccctgg
      601 atcgagatcg gctgcctcgg cgagctcctt gagctcttgg gcgagacgct ggcgcgtcag
      661 atgtccctcg cggctgcgcg acgcaaacag ccagcgcgtc cttaccatcg gcgtcaggcc
      721 gggatcgtcg aggccggcat tgagatagcg cgccagggcc tcgcgtgcgg ccggtgtcag
      781 cggcaccatg cgctcgcgcc cacccttgcc cttgacgatc agcaccttgc catcgccgtc
      841 gagcaccgac cgcggcaagc ttacgagctc cgagacgcgc agccccgtgg cgtacaggac
      901 ctcgatcagt gcgttgagcc tgagtgcccg cagccgatcg cgaccgcgcg cggcctgcgc
      961 cctggcgccg gccgccgcga tcaggcgatc gacatccgcc accgacaaaa ttttgggcag
     1021 cgtccggctc tttctcggac cgacgacgcg gcgcgccgga tcatcgccaa tgacgccctc
     1081 ggcggcgaga aacttaaaca gctgccgcac ggccgaaagc cgtcgcgcgc gggtgcttgg
     1141 cgccatctcc gcctcggcca ggtagcgctg ataggcgaca atgtcacggc tgtcggccgc
     1201 ggcgggggcc ttgccgcggc gctcgagatg gtcgaggaag tgcgtcaagt cgctgcggta
     1261 ggcagctacc gtgttggctg ccgccccgcg ctcggcggcc tgcatctcca aaaagccctc
     1321 aagaatttcg cgtgtggttg ccaccagccc cccggtcagc aagctaagca gcggcgctag
     1381 cgccggacct tgacccccgg caacggtgtg gccgtttcgc gctgggacgg ttcaaaaaac
     1441 accgccatga cataaagacc accgatcacc gcggccccaa tgatgccgac gaccacgagg
     1501 aaccggataa ggctcggcat ctggcaacgc tgctcctgct tccggtaacc gttcggtcca
     1561 aacggcgccc cgacccgccg aatgggggat taaggctcgc cgcgccgggc gctccggaac
     1621 agcgtgtggc tttgaggcct acccgcaaac ctgataccag aaagcgtggg gagtggcatc
     1681 aatggccatc ttacaggaca gagatgatgc gattcggcta cggaggcaaa aaggaggagc
     1741 aaatcgccca gctcgctgcc aaggtccgcg ccggcttggg cacccgctcg gttgtgctgg
     1801 tgggcatgcc cggttgcggc aaatcggcga tcgggcggcg gctggccagt cgcctcgaaa
     1861 tgccctttgt cgatgccgat gatgagatcg aactggccgc cggcaaggcc atcaccgaca
     1921 ttttcaagga ccacggcgag ccttatttcc gcgatggcga gcgcaaggtg attgcgcgca
     1981 ttctcaactc cggctgccag gtgcttgcga ccggcggtgg cgctttcatg agcgccgaga
     2041 cccaagacaa tatccgccag gcggcggtct cggtttggct caaggccgag ctgtcgttgc
     2101 tgttacggcg cgtgctcaag cgcaataatc gccccttgct cgagcgcgat ccggaaggtg
     2161 tgatgcgttc gctcgtggaa acgcgctatc cgatctacgc caccgccgac attacggtcg
     2221 agagccgaga tctgccgcac gacgtcatgg tcatcgagat catcgaggcc ttggcggcgc
     2281 atgccttgcc gcggcaaccg gtc
//
LOCUS       DASTMT010000138         9137 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_1061684, whole genome shotgun sequence.
ACCESSION   DASTMT010000138 DASTMT010000000
VERSION     DASTMT010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 9137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9137
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1061684"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1515)
                     /locus_tag="VFR00_02910"
     CDS             complement(<1..1515)
                     /locus_tag="VFR00_02910"
                     /inference="COORDINATES: protein motif:HMM:NF013173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HEU0158232.1"
                     /translation="MGTVAEENPAIDMPVEWTLRSAGRHVRNPLAIYAGLALAGMALI
                     ILTVAPLASKVVQRWSQRDVELRSRLVFNSIRDQVVTGLTQSNGSNLVAFFNRIAEDE
                     RLLALGFCSDAGQLLHATKRLPPSVDCASVARTKNDTLATVFDAGRRLLVSAFPMSAG
                     ASRGHFIILHDLSFVDERAAKARLYAATALVSGMVGIGLLAAAIMLGALRGWQQVVRS
                     ALADLRHGGEPSARGRDLLPFPSEMRLLLRDLRAERREGDGLQVQWSPQTLSLLLEER
                     LPDTEVIAVSNREPYIHNRKDGGVALQIPASGLVAALEPVMRACGGKWIAHGSGSADR
                     ETVDARDHIRVPPGDPAYTLRRVWLSEEEQDGYYYGFANEGLWPLCHIAFVRPTFRLE
                     DWQQYVAINQRFAAAVAQEASRDDPIVLIHDYHFALLPAMVRQHLPNATILTFWHIPW
                     PNSETFSICPWREEIMHGLLGSTILGFHTQFHCNNFLESTDRFVESRIDREQASV"
     gene            complement(1721..3157)
                     /locus_tag="VFR00_02915"
     CDS             complement(1721..3157)
                     /locus_tag="VFR00_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007532566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HEU0158233.1"
                     /translation="MPEQAQRASRAGAMARARDYVEDGSFERELARRVACKTESQKLP
                     DSLPHLRAYLEAEMVPAFARLGFSSQIYDNPLPGQGPVLLASHFEDASLPTVLGYGHG
                     DVIRGLEDQWTKGKGPWILARDGERLYGRGTADNKGQHSINMAALAAVKAERGGRLGF
                     NAKFIIEMGEEAGSKGLAELVASHKAHFAADVLVASDGPRVKPQRPTLSLGCRGALNF
                     DLVCDLREGGHHSGNWGGLIANPGILLAHAIASITGAKGELLVDALQAPPMSPAVKAA
                     LSDVEIDAGEEGPAIDTWWGEPQLSPAERVYAGNVFNVLAFTTGTPGRPVNAIPPKAV
                     ANCQLRFVAGTDTRSILPALQQHLDARGLQQVHVTPPPAANSGGFAATRTEPDHPWVA
                     FVAQSLQRSDNSKPALIPQTGGSICNDVFTDILGIPTIWIPHSYAACSQHAPDEHLLL
                     PLARSAVQLMAGLYWDIGDGNAPRAARR"
     gene            3368..3613
                     /locus_tag="VFR00_02920"
     CDS             3368..3613
                     /locus_tag="VFR00_02920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0158234.1"
                     /translation="MLSKKRCRHTGVVNFFADDPHLPVGSAIKVGRRVGYHWRCYADP
                     CNGAGAAPSLKSAEREIAQLCRQATRSRRPRRAALAH"
     gene            complement(3686..3886)
                     /locus_tag="VFR00_02925"
     CDS             complement(3686..3886)
                     /locus_tag="VFR00_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0158235.1"
                     /translation="MSFEDKEAELGLLLTRMQNEPEDRHELYLQIRQKLNELKAYGMP
                     LPETLVNLERQLEAEFEAEQGD"
     gene            4021..4284
                     /locus_tag="VFR00_02930"
     CDS             4021..4284
                     /locus_tag="VFR00_02930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0158236.1"
                     /translation="MRRALAIASSAALLWAASVGASLGQGAPSPQPGPTNPSMPSTNP
                     QAQPGATIVVNPTLEECRQGWNATMKWTKEQFQHFCTTLQSSK"
     gene            complement(4290..4889)
                     /locus_tag="VFR00_02935"
     CDS             complement(4290..4889)
                     /locus_tag="VFR00_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HEU0158237.1"
                     /translation="MRQPPLRTRAPSSIRLAGVLALGLLSALWGRPAPADSAKALAYD
                     FSFEAIEGGRMPLEQWRGKVLLVVNTASFCGFTPQYEGLQALWRRYLERGLVVIGVPS
                     NDFGGQEPKAEAEILGFCKGAYNVSFPLTSKQVVSGGEAHPFYRWAAESLGAGATPKW
                     NFHKYLVGRDGRLLGAFPTRVSPQAPELIQAIEAALAKG"
     gene            complement(4987..5634)
                     /locus_tag="VFR00_02940"
     CDS             complement(4987..5634)
                     /locus_tag="VFR00_02940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0158238.1"
                     /translation="MISPRWVRFLRCTALPAWVLALGLVCLSRAEALEEQAGEKKALD
                     ACDRKLCAMLVDKKPVGEDLKCSLTKTWAKATIKSAETQTTKWGFGDARCTVQLHMSR
                     AELVGALTAAEYKLKARPHTVDCVVEEDGAAKPIKATLAPKIEFKNGKAEKIRINLEK
                     IDGPFAITSMLSAAATLEDRIGLFHRSMLKSVNRYIERHCVNLLATAAKDGAGQK"
     gene            5928..6803
                     /locus_tag="VFR00_02945"
     CDS             5928..6803
                     /locus_tag="VFR00_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEU0158239.1"
                     /translation="MLDFIQQLVSGIALGCVYGLIALGFVLIYKATEVVNFAQGDLMM
                     LGGFFAYTFISLLGLGYWWGFLLAVLSMGLFGMLIERLLVRPILGYPQFAIVMATIGL
                     GYFLRSAAGMIWGTDDLKIDNPFGSGVIRIGNLVLAHDKLSVIVATLILCATLYLFFN
                     QTRLGTAMRATSENMLAAYYMGIPVKRVVSLIWAISAMVAACAGVLLAPITFIHTNVG
                     LALGLKAFPAAVLGGFGSIPGALAGGIIIGVIETMAGFYLPAGWKDVAPYLVLLGVLL
                     LRPEGLFGMVARKKV"
     gene            6819..8042
                     /locus_tag="VFR00_02950"
     CDS             6819..8042
                     /locus_tag="VFR00_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018643392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEU0158240.1"
                     /translation="MQFFFKTTYDQDLRLIQHRGQLIAYLLLLGALAAAPLLLSSYLQ
                     SQFVFVFIYAIVGVALMILTGFTGQGSLGHAAFLAIGAYTTAYLQSKGVPFAGAGLFT
                     LDLNLPWIGPSKLWLPHLGYFLWAGLLTGIIGAIVGFPALRLHGIYLLIATISFAFIV
                     EEILARWESVTHGNEGLRVRALQAWGLEIPTNQVSFYYLCLAVLVLVIIGTLNLMRSP
                     TGRAFIAIRDSETAAKSMGIDIAAYKVKSFAISAAITGLAGSLYAHKLSFISPEMFTL
                     LLSIEFVMVIIIGGAIGLHGAVLGAIFIVMVDPLLTWVKDDVPSIAAGIANVLGLGAG
                     RAQALEQSLNSIASAAGLKGAIYGVIIMLFILFEPMGLYGRWLKIKLFFQLFPLYKRA
                     SFKRQKTYVKTERNR"
     gene            8039..8797
                     /locus_tag="VFR00_02955"
     CDS             8039..8797
                     /locus_tag="VFR00_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002712809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0158241.1"
                     /translation="MSFFRVDGVSLQFGGLKAVDNVSFEVKEGEIFTIIGPNGAGKTS
                     IFNLISRLYDPSAGQLYFGDRDITALPAHTIAKMGIARTFQNIELFENATLLANLLVG
                     RHCHTRTRLWQELLFLPTVKRAERQHRAKVEEVIDLLELQAYREKIISGLPYGVRKVT
                     ELARALCSEPKLILLDEPSSGLNVEETDDMSFWIRDIKNELGITVLMVEHDMTLVNRV
                     SDRVLALNYGRVLASGTPAEVQSHPDVIAAYLGT"
     gene            8802..>9137
                     /locus_tag="VFR00_02960"
     CDS             8802..>9137
                     /locus_tag="VFR00_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU0158242.1"
                     /translation="MLAADANSDAILRVANIESYYGPIMAIRGVSLEVPRGKIVTVLG
                     ANGAGKTTVLKTISGVLDPQKGSIELDGRPIQRLSPDRIVRMGLGHVPEGREVFPFLT
                     VAENLKMGAY"
BASE COUNT         1533 a         3004 c         2920 g         1680 t
ORIGIN      
        1 gaccgaggcc tgctcgcgat cgatccggct ttccacgaag cgatcggtcg attccagaaa
       61 attgttgcaa tgaaattgcg tgtggaagcc caggatggtg ctgcccagca agccatgcat
      121 gatctcctcg cgccaggggc agatcgagaa ggtctccgaa ttgggccagg ggatatgcca
      181 aaacgtcagt atggtggcgt tgggcaggtg ctgacgcacc atcgccggca gcagggcaaa
      241 gtgatagtcg tggatgagca cgatcgggtc gtcgcggctt gcctcctggg cgaccgcggc
      301 ggcgaaacgc tggttgatcg ccacgtattg ttgccagtcc tccaaccgga acgtcggccg
      361 cacgaaggcg atgtggcaca gcggccagag gccctcgttg gcaaagccat agtagtagcc
      421 gtcctgctcc tcctcgctga gccagacgcg ccgtaaggta taggccggat cgccgggcgg
      481 cacacggata tggtcgcgcg cgtccacggt ctcacggtcg gcgctaccgc taccgtgcgc
      541 aatccacttg ccgccgcagg cgcgcatcac cggttccagc gccgccacca gcccgctggc
      601 cgggatctgc agcgccacgc cgccgtcctt gcgattgtgg atatagggct cgcggttgga
      661 gacggcgatc acttcggtgt ccggcagacg ttcctccagc agcaggctga gcgtttgggg
      721 tgaccactgc acctgcaggc cgtcaccctc ccggcgctcg gcgcgcaggt ctctgagcag
      781 cagccgcatt tccgagggga aggggagcag atcgcgtccc cgcgcgctgg gctcgccccc
      841 gtgccgcaga tcggccaggg cagagcgcac gacctgctgc cagccccgca atgcgcccag
      901 catgatcgcg gcggcgagga ggccgatgcc gaccatgccg ctgaccaaag ccgtggccgc
      961 gtagaggcga gccttggcgg cgcgctcatc cacgaagctg aggtcatgca aaatgataaa
     1021 gtggccgcgc gaggcccccg cgctcatggg aaaggcgctc accagcaaac gccggccggc
     1081 atcgaacacc gtggccaagg tgtcattctt ggtgcgggcc acgctggcgc agtccacgct
     1141 cggcggcagc cgtttggtgg catgcagcaa ctgccctgca tcgctgcaaa agccgagcgc
     1201 cagcaggcgt tcgtcctcgg cgatgcggtt gaagaaggcg acgagattgc tgccgttgct
     1261 ctgggtcagc ccggtgacca cctggtcgcg gatggaattg aaaaccagcc gggacctgag
     1321 ttcgacatcg cgctgcgacc agcgctgcac gaccttggag gcgagcggcg ccaccgtcag
     1381 aataatgagc gccataccgg ccaacgccag gcccgcatag atagcgagcg gattgcgcac
     1441 gtggcgcccg gcggagcgta gcgtccattc tacgggcatg tcgatggccg ggttctcctc
     1501 tgcgaccgtg cccacccaga gcccactgcg cgtgccccgt gattccgcgc tttttatgga
     1561 gatgatgcgg caacagcatg gtcgagatcc gcccacgccg tgacaggccg cgttatcggc
     1621 cggcgcgcca cgggccaggg caggtgccaa ggcgccattt ttgccggcgg gccgctgctg
     1681 ccgcgccgtg ctaggggcaa gtccgaagcc ccggggtcgc tcagcggcgg gccgcgcgag
     1741 gcgcgttgcc gtcgccaatg tcccagtaga gcccggccat caactggacg gccgagcgcg
     1801 ccaacggcag caagagatgc tcgtcgggcg cgtgttggga acaggcggca taggagtgcg
     1861 ggatccaaat cgtggggatg cccaggatgt cggtgaagac gtcgttgcag atcgagccgc
     1921 cggtctgcgg gatcagcgcc ggcttgctgt tgtcggagcg ctgcagcgac tgggcgacaa
     1981 aggcaaccca ggggtgatcg ggctcggtgc gcgttgcggc aaacccgccg ctgttggccg
     2041 ccggcggcgg ggtcacgtgc acctgctgca agccgcgcgc atcgaggtgc tgctgcaggg
     2101 cgggcaggat cgatcgcgtg tcggtgccgg cgacgaaacg cagctggcag ttggcgacgg
     2161 ccttgggggg tatggcattg accggccggc ccggcgtgcc ggtcgtgaag gcgaggacgt
     2221 tgaacacgtt gcccgcatag acccgctcgg ctggcgaaag ctgcggctcc ccccaccagg
     2281 tatcgatggc ggggccctct tctccggcat cgatctcgac gtcgctgagc gccgccttga
     2341 cggcgggcga catcggcggc gcctgcaagg catcgaccag gagttcgccc ttggcgccgg
     2401 tgatggaggc gatggcatgc gccagcagga tgcccgggtt ggcgatcagc ccgccccagt
     2461 tgccggagtg atgcccaccc tcgcgcaagt cacagacaag atcgaaattg agggctccgc
     2521 gacagccgag gctcaaggtg gggcgctgcg gcttcacgcg cgggccgtcg gaggccacga
     2581 gcacgtcggc ggcgaagtga gctttgtggc tggccacgag ctcggctagg cccttcgagc
     2641 ccgcctcctc gcccatctcg ataatgaact tggcgttgaa gccgagccgg ccaccgcgct
     2701 ccgccttgac cgctgcgagc gcggccatgt tgatgctgtg ttggcccttg ttgtccgccg
     2761 tgccgcgccc gtacaggcgc tcgccgtccc gggctaaaat ccaggggcct ttgcccttcg
     2821 tccactgatc ctcaagcccg cgaatgacgt cgccatgacc gtagcccagc acggtcggca
     2881 ggctggcgtc ctcgaaatgg ctggcaagca gcaccggacc ctggccggga agtggattgt
     2941 cgtaaatttg gctgctgaag ccgagccggg cgaaggcagg gaccatctcc gcctcgagat
     3001 aggcgcgcag atggggcaag ctgtccggca gtttctggct ctcggtcttg caggcaacgc
     3061 ggcgtgcaag ctcgcgctcg aaggaaccat cctccacata gtcgcgcgcc cgcgccatgg
     3121 cgccggcgcg acttgcacgc tgagcttgct ctggcatgcc agcatcctcc ttgcgcacga
     3181 aactgacgag acgcattccc acctcaagcg ggggcgacag gcaacgcttt tttgcgtgac
     3241 cgtcgcggcc ggctgccatt tgcgctcgcc gggggcacgc cgaggtcagt cgcacgctcg
     3301 tcacagtctt tggctagaag ggcgcgcgaa ggaggggaag gcaattgacg gttgagaaga
     3361 gggtcagatg ctgtccaaaa aacgttgccg ccacaccggc gtcgtgaact ttttcgccga
     3421 cgatccgcac ttgccggtcg gctccgccat caaggtcggc cgacgcgttg gctaccactg
     3481 gcgctgctat gccgatccct gtaacggcgc gggcgccgca cccagcctca agtcggcgga
     3541 acgcgaaatt gcccagcttt gccgccaggc cacccgctcg cgtcggccgc gacgcgcagc
     3601 cttggcgcac tgagccgccg cgccggcggc gcaagcgcgc cgccggcgtg cccgcgaagg
     3661 gctcggctaa cctgcgcgga tccgatcagt cgccctgttc ggcttcgaac tccgcctcca
     3721 gctgccgttc caggttcacc agggtctcgg gcagcggcat gccgtaggct ttaagctcgt
     3781 tcagcttctg cctgatctgc aagtagagct cgtggcggtc ctccggctcg ttctgcatgc
     3841 gcgtcaacag cagaccgagt tccgcctcct tgtcttcaaa gctcatggtt tgctccgacc
     3901 gtgtagccat gaaacgtgag cgtgagcaga gggttccctt gggaactgag ccgtcgccgc
     3961 cacgttgagc ctgcgagcag cgcgccccta gggtgcacgc cacaagcgaa gaggtcaaac
     4021 atgcgtcgtg cccttgcgat cgcgtcgagc gccgccctgc tgtgggccgc cagtgtcggc
     4081 gccagcctcg gccagggagc gccatcgccg cagcccggcc ccaccaatcc cagcatgccg
     4141 tccaccaacc cgcaggcgca acccggcgcc acgatcgtgg tcaacccgac gctggaggaa
     4201 tgccggcagg gctggaacgc cacgatgaag tggaccaagg agcaattcca gcacttctgc
     4261 acgacgctgc agtcgagcaa gtagcccgct caacccttgg cgagcgcggc ctcgattgcc
     4321 tggatcagct cgggcgcttg tgggctcacg cgcgtgggaa aggcgccgag caagcggccg
     4381 tcccggccca ccagatactt gtggaagttc cattttgggg tggcgccggc gccaaggctc
     4441 tccgccgccc agcgatagaa cgggtgcgcc tccccgccgg agaccacctg tttgctggtg
     4501 agcgggaaac tgacgttgta ggcgcccttg cagaacccga ggatttcggc ctccgccttc
     4561 ggctcctggc cgccgaagtc attcgacggc acgccgatca cgaccagacc ccgttccaag
     4621 taccgccgcc acagggcctg cagcccttca tactgaggcg tgaaaccgca gaaggaagcg
     4681 gtgttgacga cgagaaggac cttgccgcgc cactgctcga gcggcatgcg gccgccctcg
     4741 atcgcctcga aactgaagtc gtaggccagc gctttggcgc tgtcggccgg cgcggggcgg
     4801 ccccacagcg cgctcaagag cccgagcgcc aacacgccgg cgagccgtat cgacgatggg
     4861 gcgcgcgttc gcaacggggg ctgtcgcatc ggcctcgatc ctgctcttgc tctacctggt
     4921 ggccgctagc atgaagccgt tcggtaggga aagaaacttt gcgtgaagcc agcggcggcc
     4981 gccgcctcac ttttggcccg ccccgtcctt ggccgcggtc gccagcaggt tcacgcaatg
     5041 ccgttcgatg tagcggttga ccgatttcag catcgagcgg tggaagagcc cgatgcggtc
     5101 ctccagggtg gccgccgccg acagcatgct ggtgatggca aacgggccgt cgattttctc
     5161 gaggttgatg cggatctttt cggccttgcc gttcttgaac tcgatcttgg gggcaagcgt
     5221 ggccttgatc ggcttggctg cgccgtcttc ctcgaccacg caatcaaccg tgtgcggccg
     5281 cgccttgagc ttgtattcgg ctgccgtcaa ggcaccgacc agttcggccc ggctcatatg
     5341 gagctgaacc gtgcaacgtg cgtcgccgaa gccccacttg gtggtctgcg tctccgccga
     5401 tttgatggtg gccttggccc aggtcttggt gaggctgcat ttgaggtcct cgcccacggg
     5461 cttcttgtcg accagcatgg cgcagagttt gcgatcgcag gcatcgagcg ccttcttctc
     5521 gccggcctgc tcttccagcg cctcggcgcg cgacaggcac acgaggccga gcgccaagac
     5581 ccacgccggt aacgccgtgc accttaaaaa gcgaacccaa cgggggctga tcatgggccg
     5641 ggcgctccct cctccgccac tttctgatcg ggcgaggtaa gccatcgatt ggcccaattt
     5701 gtggccgcgg cgtcgccgca gcgcctcggc cctgcttgcc ccaaggctcc ctccccccca
     5761 agtccattgc ccagcgccgc cagcgaaaaa gaggctcgac tgtcagcgcg ccttgccgca
     5821 gtctagcgtg ccgacggcga agaagcggtg cggtcaaata gcatttgggg ggcgcggggc
     5881 cgctagcggc accagccgaa cacgaaggcc aaggagacgg cagcggcatg ctggatttca
     5941 ttcagcagct ggtgagtggt attgcgcttg gctgtgtcta tggcctgatc gcgctcggct
     6001 tcgtgctcat ctacaaggcc accgaagtcg tcaatttcgc ccagggcgat ctgatgatgc
     6061 tgggtggatt tttcgcctac accttcatct ccctgctggg gctcggttat tggtggggat
     6121 tcctgctggc cgtgctgagc atggggctct tcggcatgct gatcgagcgc ctgctggtgc
     6181 ggccgatcct gggctatcca cagtttgcca tcgtgatggc gaccatcggt cttggctact
     6241 tcctgcgctc ggcagccggc atgatctggg gcacggatga tctcaagatc gataatccgt
     6301 tcggctcggg cgtgatccgc atcggcaatt tggtgctggc acacgacaag ctctccgtaa
     6361 tcgtggcaac gctgatcctc tgcgccacgc tttacctctt cttcaaccag acccggctcg
     6421 gcacggcgat gcgcgccacc tcggagaaca tgttggccgc ttactacatg ggcattcccg
     6481 tcaaacgggt cgtgtccctg atctgggcga tcagcgccat ggtggcggcc tgtgccggcg
     6541 tgcttttggc cccgatcacc ttcatccaca ccaatgtggg gctcgccctc gggctcaagg
     6601 cctttcccgc cgccgtgctc ggcggtttcg gctccattcc cggcgccctt gccggcggca
     6661 tcatcattgg cgtgatcgaa accatggccg gtttctacct gccggcgggg tggaaggacg
     6721 ttgcccccta cctggttttg ctcggggtat tgctgctgcg gccggagggg ctgttcggca
     6781 tggtagcgcg caagaaggtc tgagctttag gtcgcggcat gcaattcttt ttcaagacca
     6841 cgtatgatca agacctccgc ctcattcagc accgagggca gttgattgcc tacctgctgc
     6901 tgctgggcgc actggctgcc gccccgctgc tcttgagctc ttacctgcaa agccagttcg
     6961 tcttcgtgtt catctatgcc atcgtcggcg tcgccttgat gatcctgacg ggcttcacag
     7021 gtcagggctc gctcggccat gcggccttcc ttgccattgg cgcctatacg acggcttacc
     7081 tgcaatcgaa gggcgtcccg ttcgccggcg ccggcctctt tacgctcgat ctcaatctgc
     7141 cctggatcgg gcccagcaag ctgtggctgc cgcacctcgg ctacttccta tgggccggcc
     7201 tgctcaccgg catcatcggc gccatcgtcg gcttccccgc cttgcgcctg cacggcatct
     7261 acctgttgat tgccaccatc tctttcgcct tcatcgtcga ggagatcttg gcccgctggg
     7321 agagcgtcac gcacggcaac gagggcttgc gcgtgcgcgc cctgcaagcg tggggcctcg
     7381 agattccgac caatcaggtg tccttctact acctgtgcct ggcggtcctc gtgctcgtca
     7441 tcattggcac gctcaacctc atgcgctcgc cgaccgggcg cgccttcatc gccattcgcg
     7501 acagcgaaac cgccgccaaa agcatgggga tcgatattgc ggcctacaag gtaaaatcgt
     7561 tcgccatctc ggcggccatc acgggactgg cgggatcgct ctacgcccac aaattgtcgt
     7621 tcatcagtcc ggagatgttc acgctgctgc tgtcgatcga gttcgtgatg gtgatcatca
     7681 tcggcggcgc cattggcctg cacggagccg tgctgggcgc gattttcatc gtcatggtcg
     7741 atcccttgct gacctgggtc aaggatgacg tgccgagcat cgccgccggc attgccaatg
     7801 tgcttggcct cggcgccggc cgggcccagg cgctcgagca gagcctcaac tccatcgcct
     7861 ccgcggccgg gctcaagggc gccatttacg gcgtcatcat catgctcttt attctgttcg
     7921 agcccatggg cctttacggg cgctggctga agatcaagct ctttttccaa ctcttcccgc
     7981 tctataaacg cgcaagtttc aagcgccaga agacctacgt taagacggag cgcaatcggt
     8041 gagcttcttc agggtcgacg gcgtctcctt gcaattcggt ggcctgaagg ccgtcgacaa
     8101 cgtctccttc gaggtcaagg agggggagat cttcaccatt atcggcccga acggcgccgg
     8161 caagacctcg atcttcaacc tgatttcgcg cttgtatgat ccaagcgctg ggcagcttta
     8221 cttcggcgat cgcgacatca cggcgctgcc cgcgcacacg atcgccaaaa tgggcatcgc
     8281 ccgcaccttc cagaacatcg aactctttga aaatgccacg ctactggcca atctcctggt
     8341 cgggcgccac tgccatacgc gcacgcggct ctggcaggag ctgttgttcc tgcccaccgt
     8401 caagcgcgcc gagcggcagc atcgcgccaa ggtcgaagag gtgatcgacc tgctcgaact
     8461 gcaggcctat cgggaaaaga tcatttccgg cttgccctac ggtgtgcgca aggtgaccga
     8521 gcttgcccgc gcgctgtgct ccgaacccaa gctgatcctg ctggatgagc cttcctccgg
     8581 gctcaacgtc gaggaaaccg acgacatgtc gttctggatc cgcgacatca aaaacgagct
     8641 cggcatcacg gtgctgatgg tggagcacga tatgaccctg gtaaaccgcg tctcggaccg
     8701 cgtgctcgcg ctcaactacg gccgcgtcct tgccagcggc acccccgccg aggtgcagag
     8761 ccatcccgat gtcatcgccg cctatcttgg gacctaaggg aatgctcgcc gccgacgcca
     8821 acagcgatgc catcctgaga gtagccaaca tcgagagcta ctacgggccc atcatggcga
     8881 ttcggggggt cagcctagag gtgccgcgcg gcaagatcgt caccgtcctg ggagccaacg
     8941 gcgccggcaa gaccacggtg ctcaagacga tctccggcgt gctcgatcca cagaagggca
     9001 gcatcgaact ggacggacgg ccgatccaac gcttgagccc ggaccgtatc gtgcgcatgg
     9061 ggctcgggca cgtaccggag ggtcgcgagg tctttccttt tctcaccgtt gccgaaaacc
     9121 tcaagatggg cgcctac
//
LOCUS       DASTMT010000139         2066 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_2701959, whole genome shotgun sequence.
ACCESSION   DASTMT010000139 DASTMT010000000
VERSION     DASTMT010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2066
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2701959"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            337..663
                     /locus_tag="VFR00_02965"
     CDS             337..663
                     /locus_tag="VFR00_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015667758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEU0158243.1"
                     /translation="MTYESALSALADPTRRQVFERLRAGPLAVKAIAAGLPVSRPAVS
                     QHLKALKQAGLVAERSEGVRRIYSVRRDGLAELRDWLDGFWGDALEAFKQEAEKPRGK
                     TQRSRS"
     gene            660..1145
                     /locus_tag="VFR00_02970"
     CDS             660..1145
                     /locus_tag="VFR00_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015667757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEU0158244.1"
                     /translation="MTLSPPIAPVRKSVTVKAPIAHAFEVFTSGLARWWPPNHGIGKK
                     PIAKVLLEPRLGGHWLEISEDGTETRVATIIHWDPPHRLSMLWQVNAQWKPDTSMRSE
                     VDVRFTAEGPEVTRVELLHHKFESMGAEAGASLRKDVAGGWPGLIERFVAEAEHSHPH
                     A"
     gene            1180..1839
                     /locus_tag="VFR00_02975"
     CDS             1180..1839
                     /locus_tag="VFR00_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008964451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEU0158245.1"
                     /translation="MSSFTVHSVPGSPYGRAVLLTLEEKQVPYRLAAVAPPQMKSEPH
                     LARHPFGRVPVLEHGTFVLYETQAILRYLDRVLPTPALTPPDACAAARMDQVMNICDW
                     YLFQGVNTVIGFNRVVKPRLLGQAPDEAAIAAAMPKAHRVFDELARLLGEGPYFAGKS
                     LSLADLMVVAQIDFFRGQPEWPPLAGPHPNLSAWLDRMTARPSLEATTWERVSQMAQA
                     A"
BASE COUNT          354 a          723 c          658 g          331 t
ORIGIN      
        1 gcgacccccc gcatttcccg gggtaacgcc tggcaacacg catccccatg agtttgcgcc
       61 aggggcgcac aaggcaaggt gcccgtcgag ccgtctcgcg cgccgcctgg gaccggatca
      121 gcgcgttcat tgcgatcgcc atcgatccgc tcaaaaagtc ttggcgtcga gggcgttggc
      181 tgggccggcg acagagcgga caggagcctg ggccggaaaa gccgcggcgc ctcgcatcgg
      241 ttttgatcct gcacaagtac atggcgcggc tcggccaggt gccccggtgc aacgtcgcca
      301 gcttgactca tggtaagcta tcacttaccg taagctatga cttacgaaag tgctttgtcg
      361 gccctcgccg atcccacgcg ccgccaggtc ttcgagcgcc tccgcgccgg gccgctggcc
      421 gtcaaggcga tcgccgcggg gctccccgta tcgcgcccgg ctgtgtcgca gcacctgaag
      481 gccctcaaac aggccggcct tgtcgccgag cgcagcgagg gcgtgcggcg gatctattcg
      541 gtgcgccgcg acggccttgc cgagctgcgc gattggctcg acggtttctg gggcgatgcg
      601 ctcgaggctt tcaaacaaga agcggaaaaa ccaagaggta agacgcagag gagccgatca
      661 tgaccctgtc accccctatc gcgcccgtgc gcaaaagcgt gaccgtcaaa gcccccatcg
      721 cccacgcctt cgaggtcttc accagcggac tggcgcgctg gtggccaccc aaccatggca
      781 tcggcaaaaa accgatcgcc aaggtgctgc tggagccgcg cctcggcgga cactggttgg
      841 aaatttccga ggacggcacc gagacgcgcg tcgccaccat catccattgg gacccgccac
      901 atcgcctgag catgctctgg caggtcaacg cccaatggaa gcccgacacc agcatgcgct
      961 cggaggtcga cgtgcgcttc accgccgaag gaccggaggt cacccgcgtc gagctcctgc
     1021 accacaaatt cgaaagcatg ggtgcggagg ccggcgcctc gctgcgcaag gatgtcgccg
     1081 gcggctggcc cggcctgatc gaacgcttcg ttgcggaggc cgagcacagc cacccgcacg
     1141 cttaggggat gatggtccag atcacaagga ggattgccca tgtcgtcgtt caccgttcac
     1201 agcgtgccgg gaagtcccta cggtcgcgcc gttcttctca cgctggagga aaaacaggtc
     1261 ccctatcggc tcgccgccgt ggcgccgccc cagatgaaat cggagccgca tctcgcgcgt
     1321 caccccttcg gccgcgtgcc ggtgctcgag cacggcacgt ttgtcctcta cgaaacgcag
     1381 gcgattttgc gctatctcga ccgcgtgctg ccgacgccgg cgctgacgcc gcccgatgcc
     1441 tgtgccgccg cgcgcatgga ccaggtcatg aacatttgcg attggtacct gtttcagggc
     1501 gtcaacaccg tcatcggctt caatcgcgtg gtgaagccgc gcctgctcgg acaggcgccc
     1561 gatgaggccg ccatcgccgc cgccatgccg aaggcgcacc gggtgttcga cgagctcgct
     1621 cgtctgctgg gcgaggggcc ctatttcgcg gggaagagcc tttcccttgc cgatctgatg
     1681 gtcgtggcgc agatcgactt cttccgcgga caaccggagt ggccgccgct cgccggtccc
     1741 catcccaacc tctccgcctg gctcgaccgc atgacggccc gcccgagcct cgaggccacg
     1801 acctgggagc gcgtcagcca aatggcccag gccgcctgag ccgttcaggc cacgcgctgg
     1861 cggcccgcac cgggaatggc cacccgagag ccacggccgg ggtgcgcgtc gccggcgctg
     1921 catcacggag cgacgaagcc ttttcctaag gctccgtctc ccccatggcg gcggcgaacg
     1981 gaagccccaa tgcctggcgc gcctcacagg ggtttgagcc cgcccagcaa ggtcggcaga
     2041 aactccgcca ccgttggatg gatgtg
//
LOCUS       DASTMT010000140        14053 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_5946658, whole genome shotgun sequence.
ACCESSION   DASTMT010000140 DASTMT010000000
VERSION     DASTMT010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 14053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14053
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5946658"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..737)
                     /locus_tag="VFR00_02980"
     CDS             complement(<1..737)
                     /locus_tag="VFR00_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019964133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEU0158246.1"
                     /translation="MTAASLSKKAVQRLSEAEATAELARLAGEIARHDALYYGEDAPE
                     ISDADYDALRQRNAAIEARYPQLVRTDSPSRRVGSAPAEAFGKVRHAVPMLSLGNAFN
                     EEDVSDFMARVRRFLGLAAEAVVEVTAEPKIDGLSISLTYSKGRLTQAATRGDGTEGE
                     NVTPNVKTMRQIPQHLRGAALPDLIEVRGEIYLAHQDFVALNREQAASGGKIFANPRN
                     AAAGSLRQLDASITARRPLRFFAYGWGA"
     gene            complement(799..1749)
                     /locus_tag="VFR00_02985"
     CDS             complement(799..1749)
                     /locus_tag="VFR00_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013945989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HEU0158247.1"
                     /translation="MACALLGLLLTGCASSNDAAKALNPDPPGKMYADAEALLNKGEW
                     TLAAKKYEDLDRDHPYAPEARRAIVMAAYAYYKGAKYPEAITSAQRYTTLHPGTKDAA
                     LAHHIIASANFDDIKDPARDQTATRKALAELKVLRQRYPDSPYAKQAENRIRIAEDVL
                     AASEMNVARYYQKQGNQLAAINRYRTVVTDYQTTAHVEEALYRLVETNMVLGIVPEAQ
                     SAAAVLGHNYPNSEWYQNAYALLKSGGLSPQMNTGSWISNTLKALTPGGQKKPAPAPQ
                     QPAPGLPAPDQLPAPRVPGEDVPTAAKPNGRPPLGFAQSQ"
     gene            complement(2039..2992)
                     /gene="lpxC"
                     /locus_tag="VFR00_02990"
     CDS             complement(2039..2992)
                     /gene="lpxC"
                     /locus_tag="VFR00_02990"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015596480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HEU0158248.1"
                     /translation="MSRRFIGSRQTTIAHEIQLRGTGVHSGAPVNLVLHPAEADTGFR
                     FLVSRRGRIIADIAAHIESVKNLTLCTVLGDEAGVTVSTVEHLLSALRGLNVDNCFIE
                     IDAREVPIMDGSAASFVSAIDQTGIRELAQPRKFIKVLKPIRVEEGDCWGMLSPHSSF
                     HVDVEIDFATALIGRQRMALEMNAGAFRNEICRARTFGFMRDVESLWKAGLALGASLE
                     NTVALADDRIVNSEGLRYPQEFVRHKILDAVGDLALAGAPMLASYRSVKGGHRLNARV
                     LQALFADREAWTVVRAPWVREVVPVEVSSGLAAVNYAADRS"
     gene            complement(3225..4685)
                     /gene="ftsZ"
                     /locus_tag="VFR00_02995"
     CDS             complement(3225..4685)
                     /gene="ftsZ"
                     /locus_tag="VFR00_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEU0158249.1"
                     /translation="MKPRIAVIGVGGAGGNAVNNMIAAGLSGVDFIVANTDAQALEAS
                     TASTRVQLGVSLTEGLGAGSKPEIGEAAAEEAVEEIRAALAGSHMVFIAAGMGGGTGT
                     GAAAVIARAAREFGILTIAVVTKPFQFEGSRRMRVAEAGIAELRGHVDTLLVIPNQNL
                     FRVANDRTTFAEAFVLADQVLYSGIACIVDLIIKDGLINLDLADVRTVLSGMGPAMMG
                     TGEASGEQRAVVAAEEAIVNPLLDDVTLKGAKSLLLSITGGAKLTLWEVDEAANRVRK
                     EVDPEANIIVGATFDEALGERVRVSIVASGMGLSKTGRLAPEAAAAAELEAGKDNAPR
                     TPDFRWRLSQAMTAPEGEGPALPPTGAPAWDAPGGVRITEGPPRLAGEPADRPSESHP
                     AHDRFPGLTDFPPHAQRAYRAMLRTAPPGAEPARSAVYRQPPTDSVLGKFGLLRRLAD
                     AARGRAQEQPASPLSGPQETAERVDLPAFFGRGKPS"
     gene            complement(4791..6053)
                     /gene="ftsA"
                     /locus_tag="VFR00_03000"
     CDS             complement(4791..6053)
                     /gene="ftsA"
                     /locus_tag="VFR00_03000"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEU0158250.1"
                     /translation="MAPAHTFNRQTPPLLGLLDIGTSKTVCFIVAAPRKGRAGRVDVL
                     GIGQQPSRGLKAGVVIELDAAEHSVRAAVAEAERAAGCELRQVLLAVACGRLKSTTFA
                     ADTKVEGRAVGDADLARLMSAGRSYVERDGRALLHMNTIAYRLDGAHGIADPRGLAAD
                     TLSVDLHAVTADDAPLRNLVHVAERAYLSVAGLVPAPYASGLAVTTPQERRAGVLVLD
                     FGAGTTSLALFVDGHLLASEVIPVGGHHISFDLARGLTTSLSEAERIKALCGTVPPGP
                     DADATPVSFAPVGEPAAPLSEVASSKVREIICGRVRGLLGQVAERLERAGIPEGATER
                     VVVTGGAGQLPHLSAFAGAFFGKPVRVGQPEAAYGWPAGAASPAFSTVLGVAQVAFDP
                     AAGVRRAEHNFAAGGYLRSVGRWLKASF"
     gene            complement(6071..6844)
                     /locus_tag="VFR00_03005"
     CDS             complement(6071..6844)
                     /locus_tag="VFR00_03005"
                     /inference="COORDINATES: protein motif:HMM:NF015739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HEU0158251.1"
                     /translation="MTLARRSLPVLLALAALLLAAGVKLAHSETGARLFAKLEIWAQV
                     AGLGLDQVAVIGHRYTADKDIFEALELRRARTMLSFDPQRARSRLSELPWIEAASIER
                     VFPDRLEVRIKERTPVAVWSRGERHALIDAQGRSLASVPAEVMPQLPRVGGEGADAAA
                     AELFVLLQRFPELKARVASAERIGDRRWTLRLRGGGAVQLPASSEAEALSEAGRLLAK
                     GFERAEIDVRSPARAIVREAPRLDGAQLMASERRPSERL"
     gene            complement(6955..7899)
                     /locus_tag="VFR00_03010"
     CDS             complement(6955..7899)
                     /locus_tag="VFR00_03010"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003615781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEU0158252.1"
                     /translation="MTSPGRRFQHVAVLLGGFSAEREVSLRSGKACADALEQAGYRVT
                     RIDAQRNLGEQLSQARPEVCFNALHGRWGEDGCVQGLLEVLGLPYTHSGVLASALAMH
                     KQRAKSVLQAAGVPVPGGRVVRRIEAARAHALARPYVLKPVAEGSSVGVYIVREDHLH
                     PPQELNDPSWAYGEELLAEPFIAGRELTCAVLGDRALGVTEIVPAEALRFYNYEAKYA
                     DGGSRHLLPAPISSNVYELVQKLTLTAHRALGCRGVTRADFRYDDTPGGTGELACLEV
                     NTQPGMTGTSLVPELAAHAGLSLPALVTWMVEEASCNR"
     gene            complement(7896..8861)
                     /gene="murB"
                     /locus_tag="VFR00_03015"
     CDS             complement(7896..8861)
                     /gene="murB"
                     /locus_tag="VFR00_03015"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEU0158253.1"
                     /translation="MTLPDLSARLAAELPALRGRLLGNVRLADLTWFRVGGPAEVLFS
                     PADADDLAYFLSRLPASIPTFVLGLGSNLLVRDGGVPGVVIRLGRGFAHTQVEADSHL
                     RVGAAVPDVKLARAAADAAIAGLAFYRGIPGSIGGALRMNAGAHGRETRDVIRAASAV
                     DRQGRQHVLTAADLNLSYRHCGVPDDWIFTEALYQGCPGSSAEILQEMAEVADYREAH
                     QPIKERTGGSTFKNPSAARAWQLIDQAGCRGLSVGGAKVSEMHCNFLINGGAATAEDI
                     ERLGETVRARVLAVCGVTLHWEIIRQGLPSEGRPIGEALAKALAP"
     gene            complement(8858..10294)
                     /gene="murC"
                     /locus_tag="VFR00_03020"
     CDS             complement(8858..10294)
                     /gene="murC"
                     /locus_tag="VFR00_03020"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEU0158254.1"
                     /translation="MQMPAKIGTVHIVGIGGIGMSAIAEILHAKGYVAQGSDQKESAN
                     VKRLRAKGIRVFVGHDVVNLVGAKFVVVSTAVKPGNVELEAARAKGLPIIGRAEMLAE
                     LMRLYATVAVTGTHGKTTTTSLIAHLFRAGGKDPTVITGGIVNDWGSNARLGDGQWMI
                     VEADESDGTFIKIPTQIGIVTNIDPEHLDYFKTVENMHQQFATFLRNIPFYGLAVACI
                     DHPVVRDMLEQLEMRRNGRRLLTYGTHPGADLLLKQARVEGAHTLFDADLGSRVAGGA
                     RSLRGWSVPIPGQHNALNALAAIAAACEAGIPDDTIRAAIASFAGVKRRFQPTGSWNG
                     IAIYDDYGHHPVEIAAVLKAARAGAKGRVIAIVEPHRYTRVRDLFDEFASCFKDADSV
                     ILTPLYSAGELPIAGVDHTALAERVRATGHRSVATVSSERDLPAALRFAARAGDTVVC
                     LGAGNSTEWAHALPQWLAAEPVPAGGVA"
     gene            complement(10351..11472)
                     /gene="murG"
                     /locus_tag="VFR00_03025"
     CDS             complement(10351..11472)
                     /gene="murG"
                     /locus_tag="VFR00_03025"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HEU0158255.1"
                     /translation="MASVRGPSVVLAAGGTGGHLFPAFALAEELGRRRLAVDLVTDAR
                     GGRYGVDFPARAIHRLPSATLAGRSLCAAAKTGTTLMRGMAGAFGLFRRLRPHAVVGF
                     GGYPAFAPVVAARVLGIPTLLHEQNAVLGRANRFLAAYVTAVATSFENTKFLKIGRRP
                     MVRHVGNPVRAQVLQWADQPYRAPEARDPFRLLVFGGSQGARAFSELLPPALARLPAA
                     ARDRLVVVQQCRAEDLDGVKAAYQQQGVRAHLESFFPNLPEEMAQAHLVIARAGASTV
                     AELAVLGRPAILVPLPHAIDNDQRLNAMRLAESGGAWCIEQGDLSPERLAASLAEVMA
                     APARLVVAAAAAKRQGRPDAVARLADLVEELIGNPRHGA"
     gene            complement(11460..12635)
                     /locus_tag="VFR00_03030"
     CDS             complement(11460..12635)
                     /locus_tag="VFR00_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013945979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative peptidoglycan glycosyltransferase FtsW"
                     /protein_id="HEU0158256.1"
                     /translation="MTLSRAERSRLAEWWFSVDRVLLATVLVIAGTGMLLSLAASPAI
                     ALKRGLPTFYFVERHFLFATIALATMFAVSLLTPAQLRRLALLVLGTSLPLMLLVLLT
                     GQEINGARRWLHIGGYTLQPSEFAKPAFVILSAWLFAEAERRPDMPATSAAVALYLLF
                     AALLVLEPDLGQTLLISLVWSALFFLANRPLKWFFLVLAALGAGLVAAYFGFGHVRWR
                     VERFLNPALGDTFQTDRALRSFGEGGLLGKGPGEGTLKTALPDAHTDFIFAVIAEEYG
                     VLACLVILGLFALIGSRVLLRQLREGDAFLRLSCSGLALLFVLQASMNMAVNVGLAPA
                     KGITLPFISSGGSSMLGVGLLFGMLLGLGRRRADPASLERPNFMASAAYGSARSWRA"
     gene            complement(12678..>14053)
                     /gene="murD"
                     /locus_tag="VFR00_03035"
     CDS             complement(12678..>14053)
                     /gene="murD"
                     /locus_tag="VFR00_03035"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006467223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEU0158257.1"
                     /translation="CLALVGLGRSGLAAARALLAGGARLKAWDDDALSRSAGAAAQIE
                     IEDLAESDWSRFAALILAPGIPLTHPEPHWSVAKARAAGVPILGDIELFCRERARAGG
                     ASPFVAVTGTNGKSTTSALLAHLLRSAGLEVALGGNIGTAILDLAPPAAECVHVVEMS
                     SFQIELTPSLAPSIGILLNISPDHLDRHGSMQHYAALKARVVESAEHPIVGDDDDWCQ
                     DISERLRLANRSWVDLVSAQSRLGHGWYALGSELIARAPWTGPLGAFADLAGVGALRG
                     RHNAQNALAASAAAMHLGVRPEQIAAALPTFPGLPHRLEEVGRIGATLFINDSKATNA
                     DSAATALAAFPGDIFWILGGKPKEGGITSLEPYFARIRKAFLIGEATPAFAATLNNKV
                     AFERCDTLEVAVVAAARDAACLGGTQPIVLLSPACASYDQFRNFERRGDAFRALVAAL
                     PRVEAER"
BASE COUNT         2414 a         4737 c         4720 g         2182 t
ORIGIN      
        1 gcgccccacc cgtaggcgaa aaaacgcaac ggccgccgag ccgtaatcga ggcatcgagt
       61 tggcgcaagg atccggcggc ggcgttgcgc gggttggcga aaattttgcc cccgcttgcc
      121 gcctgctcgc gattgagtgc cacgaagtcc tgatgcgcca aatagatctc cccgcgcacc
      181 tcgatcaaat cgggcagggc ggcgccgcgc aggtgctggg ggatctgtcg catggtcttg
      241 acattgggcg tcacattctc gccctcggtc ccgtcacctc gggtggcggc ctgcgtgagc
      301 cggcccttgc tataggtgag cgagatcgac aacccatcaa ttttcggttc ggccgtgacc
      361 tcgaccacgg cctccgccgc caggccgagg aagcggcgga cgcgcgccat gaaatcgcta
      421 acgtcctcct cgttaaaggc gttaccgagc gacagcatcg gcacggcatg acgcaccttg
      481 ccgaaggcct ccgcgggtgc cgagcccacc ctgcgcgagg gcgagtcggt gcgcaccagc
      541 tgtgggtagc gcgcctcgat ggccgcgttg cgctgacgta gggcgtcgta gtcggcatcg
      601 gagatctccg gtgcgtcctc gccgtagtag agcgcatcat ggcgcgcaat ctcaccggcc
      661 agacgcgcaa gttcggccgt ggcctcagcc tcgctcaagc gctgcacggc tttcttggat
      721 aggctcgccg ctgtcatggc gccacactgc cccagccgcg cggtcaatgc cagaccagca
      781 gaaccgctgg atcgcgatct actgtgactg ggcaaagccc agcggcgggc ggccgttggg
      841 cttcgccgcg gtcggcacgt cctcgcctgg cacgcgcggc gctggcagct gatcgggtgc
      901 cggcaggccc ggggctggct gctgcggcgc cggcgcgggc ttcttctggc cgccgggggt
      961 cagcgctttc agcgtgttgc tgatccagga cccggtgttc atctgcgggc tgagaccgcc
     1021 ggatttcagg agcgcgtagg cgttctgata ccactccgaa ttggggtagt tgtgaccgag
     1081 cacggcggcg gccgactggg cctctggcac aatgccgagc accatgttgg tctcgaccag
     1141 gcgatagagc gcttcctcga cgtgcgccgt ggtctggtaa tcggtcacaa cggtgcgata
     1201 gcgattgatg gcggccagct ggttgccttg tttttggtag tagcgcgcaa cattcatctc
     1261 cgaggcggcc aagacgtcct cggcgatgcg gatgcggttc tcggcttgct tggcgtaggg
     1321 gctgtccgga tagcgctggc gcagaacctt aagttcggcc agcgccttgc gcgtggcggt
     1381 ctggtcgcga gcgggatcct tgatgtcgtc gaagttggcc gaggcgatga tgtgatgggc
     1441 gagcgcggca tccttggtgc cgggatgcag cgtggtgtaa cgctgcgccg aggtgatggc
     1501 ctcggggtac tttgcgcctt tgtaataggc ataggcggcc atcacgatag cgcgccgggc
     1561 ctccggcgcg tagggatgat cgcgatcgag gtcttcatac tttttggccg ccagcgtcca
     1621 ctcacccttg ttgagcaagg cctcggcgtc ggcatacatt ttgccgggcg gatcggggtt
     1681 cagcgcctta gcagcatcgt tgctggaggc acaccccgtc aacagcaggc ccagcaacgc
     1741 gcaggccaag gccaatcgcg gcccgggcag cgctctggct cgcccctgtg ttcctgcccc
     1801 aacgacggcc atgcgcccag tccccgttgc ggtcgctccg gttcttgaga ggccggattg
     1861 cgacgtcttt tagccaccct gcggccgact ctgcacggcc atagcatagg acgcggcacc
     1921 gagcacgtcc ctcgcgcacc accccctcga gtttgcccgg actttgccgc caaattgtgg
     1981 acagcaggcc gccgtgatct gccgttgacg cgcggccgca atggccgcgg cccaagcttc
     2041 aggaccggtc ggccgcgtag ttgaccgccg ccaggccact cgagacttcg acgggaacga
     2101 cctcgcgcac ccagggcgcg cgcacgaccg tccaggcctc gcgatcagca aacagcgcct
     2161 gcagcacgcg cgcgttgagc cggtgaccgc ccttgaccga gcggtaggac gccagcatgg
     2221 gcgcgccggc cagggccaaa tcaccaaccg cgtcgaggat cttgtggcgc acgaactcct
     2281 gcggatagcg cagtccctcg ctattgacga tgcgatcgtc cgccagggcc accgtgttct
     2341 ctagcgaggc gcccagggcg aggcccgcct tccagaggct ctcgacatcc cgcataaagc
     2401 caaaggtccg agcccggcag atctcgttgc ggaaggcgcc cgcgttcatt tccagcgcca
     2461 tgcgctggcg cccgatcagg gccgtcgcaa aatcgatctc gacgtcgacg tggaagctcg
     2521 aatgcgggct cagcatcccc caacagtcgc cctcctcgac gcggatgggc ttgagcacct
     2581 tgatgaactt gcggggctgc gccaattcgc gaatgcccgt ttgatcgatg gcgctcacga
     2641 agctggcggc gctgccgtcc atgatcggca cttcgcgggc gtcgatttcg ataaagcaat
     2701 tgtcgacgtt gagcccgcgc aaggccgaga gcaggtgttc cacggtcgac accgtcaccc
     2761 cggcctcgtc acccagaacg gtgcataggg tcaggttctt gacgctttcg atatgcgccg
     2821 caatgtcggc gatgatgcgt ccgcggcggc tgaccagaaa gcgaaagcct gtgtcggcct
     2881 ccgcgggatg aagcaccagg ttaaccggtg caccgctgtg cacccccgtg cctcgtagct
     2941 gaatctcatg agcgatagtg gtctggcgcg accctataaa tcgcctggac atgtgtccct
     3001 cgatcccctc atcggcagaa cgccgcccgt ttcggcgttc cgcgccccca aaaaaatcga
     3061 cccccgctgc tcgtcttgaa acccagagct ctttggctct cacgctcaac cggcgtttgt
     3121 tgaatcaaac gatactggag tgtgccgatt ggggacaaat cacggtttct tacactgtgt
     3181 taccgctgcg ccgcagctcg ggccggagcc caccggcggc gcgctcagga cggcttgccg
     3241 cgcccaaaaa acgcgggcaa atcaacgcgc tccgcggttt cctgcggccc ggatagaggt
     3301 gacgcgggct gctcctgcgc gcgaccgcgc gcggcatcgg caaggcgacg cagcaggcca
     3361 aattttccta acaccgaatc agttggcggt tgccggtaca cggctgaccg tgccggctcg
     3421 gcgccgggcg gcgcggtgcg cagcatcgcc cggtaagccc tttgtgcgtg cggaggaaaa
     3481 tccgtcaagc cgggaaagcg atcgtgtgcg ggatggctct cgctaggccg gtcggcgggc
     3541 tcgccggcga gccttggtgg gccctccgtg atgcgaacgc cacccggcgc atcccaggcc
     3601 ggcgctccgg tcggcggcag cgccggaccc tcgccctcgg gcgcggtcat ggcctgcgac
     3661 aagcgccaac gaaaatcggg agtgcggggg gcgttgtctt tgccggcttc gagctctgcg
     3721 gcagccgccg cctctggcgc caagcgtccg gttttgctca gacccatgcc ggaggcaacg
     3781 atggacacgc gcacgcgctc acccaacgcc tcatcgaagg ttgcgcccac aataatattg
     3841 gcttccgggt cgacttcctt gcgcacgcga ttggcggcct catcgacctc ccagagcgtc
     3901 agcttggcgc cgccggtgat ggagagcagc aggcttttgg cccccttgag ggttacgtcg
     3961 tcgagcaacg gattgacaat cgcttcctcg gcagccacca ccgcgcgctg ctcgccggag
     4021 gcctcgcccg tccccatcat ggccggcccc atgccgctca acacggttct gacgtcggcg
     4081 agatccaagt tgatcaggcc gtccttgatg atcaagtcaa cgatgcaggc aataccggaa
     4141 tagagcacct gatcggccaa cacgaacgcc tccgcgaagg tggtgcgatc gttggcgacg
     4201 cgaaataggt tctggttggg aatgacgagc agcgtatcga catgcccgcg cagctcggca
     4261 atgcccgcct ctgcaacgcg catgcggcgc gagccctcaa actgaaaggg cttggtgacg
     4321 acggcgatcg tgaggatacc gaactcccga gcggcgcgcg cgatcaccgc ggcggcgccg
     4381 gtaccggtgc cgccgcccat gcccgcggcg atgaaaacca tgtggctgcc ggccagtgcg
     4441 gcacggatct cctcgacggc ttcttcggcc gcggcctcgc cgatctcggg tttggagccc
     4501 gcacccagac cttcggtgag gctgacgccg agctgcacgc gagtggaggc cgtcgaggcc
     4561 tcaagcgcct gcgcatcggt attggcgacg atgaaatcga cgccgctcaa gccggccgca
     4621 atcatgttgt tgacggcatt gccgccggcg cctcccactc cgatcacggc tatccgcggc
     4681 ttcatgtctg tcgccctcgc ccttgccact gcctagctgc gcccgcgcga gcttccattt
     4741 cgttgccttg cctcgccgcg cccaccgttg ccgccgcgcc tggcctcacc tcaaaaagag
     4801 gccttgagcc agcgaccgac gctgcgcaaa tacccgccgg ccgcaaagtt gtgctcggca
     4861 cggcgcacgc ccgcggctgg atcgaacgcc acttgggcga cacccaagac cgtggaaaat
     4921 gcgggactag cggcacctgc cggccatccg taggccgcct cgggttggcc aacgcgcacc
     4981 ggtttgccga agaacgcacc ggcgaacgcg ctcaagtgtg ggagctgccc cgctcctccc
     5041 gtcaccacca cgcgctcggt cgccccctcc gggatgccgg cccgctccag ccgttcagcg
     5101 acctgaccca agagcccacg cacacgacca cagatgatct cgcgcacttt gctgctcgcc
     5161 acctcactca gcggcgcagc gggttctccg accggcgcaa acgagacggg cgtcgcatcg
     5221 gcgtccgggc ccggcggcac tgtgccacaa agcgccttga ttcgctcggc ctccgacagc
     5281 gacgtggtca agccgcgggc aaggtcgaag ctgatatgat ggccgccgac gggaatgact
     5341 tcgctggcga gcaggtgtcc gtcgacgaac agcgcgagcg aggtggtccc cgccccaaaa
     5401 tccaagacga ggacgcccgc cctgcgctcc tgcggcgtgg tgacggcgag gccactggca
     5461 tagggcgctg gcaccagccc ggcgacggac aaataggcgc gctctgccac gtgcacgaga
     5521 ttgcgcagcg gcgcgtcgtc ggcggtgacg gcgtgcagat ccacactcaa ggtgtcggcg
     5581 gcaagcccgc gcggatcggc aatgccgtgg gcgccgtcga gtcgataggc gatggtattc
     5641 atgtgcagca acgcgcggcc atcgcgctcg acgtagctgc gcccggccga catcaggcgg
     5701 gcgaggtcgg catcgccgac agcccggccc tcgaccttgg tgtcggcggc aaaggtggtg
     5761 gacttgagcc gtccgcaggc gaccgcgagc aggacctgac gcagctcaca gcccgccgcg
     5821 cgttccgcct cggccacagc ggcgcggacc gagtgctccg ctgcatccag ctcgatcacc
     5881 acgccggcct tgagaccgcg cgagggctgc tggccaatgc ccagcacgtc gacgcggccg
     5941 gcgcgcccct tgcgcggcgc ggccacgata aagcacacgg tcttgctggt gccgatgtcc
     6001 aggagcccga gaagtggggg cgtctgtcgg ttgaatgtat gcgccggtgc cacgcgcgag
     6061 cctccacgcc tcagagccgc tcgctcggcc ggcgctcgct ggccatcagc tgcgccccat
     6121 cgaggcgggg cgcctcgcgc acaatcgccc gcgcagggga gcgtacgtcg atctccgcgc
     6181 gctcgaagcc cttggctaat agccggcctg cctcgctcag cgcctccgct tcgctgctgg
     6241 cgggaagctg caccgcaccg ccgccgcgga gccgcaaggt ccaacgccgg tcaccgatgc
     6301 gctcggcgct ggcgacacgg gctttgagct cggggaagcg ctgcagcagc acgaacagct
     6361 cggcggcggc cgcatccgcc ccctcgccac cgacgcgtgg cagctgcggc atcacctcgg
     6421 cgggcaccga ggccagcgag cgtccttgcg catcgatcag ggcgtggcgc tcgccacggc
     6481 tccagaccgc aaccggcgtg cgctccttaa tgcgcacctc caggcgatcg gggaagacgc
     6541 gctcgatgct cgccgcttcg atccacggca gctcggaaag tcgggagcgc gccctctgcg
     6601 ggtcgaagct cagcatcgtg cgcgcgcgcc taagctcgag cgcctcgaag atatctttat
     6661 cggcagtgta gcggtgcccg atcactgcga cctggtcgag gccaagcccg gccacctgcg
     6721 cccaaatttc cagcttggcg aaaaggcgag caccggtctc gctatgggcg agcttgacgc
     6781 ccgcggcgag gaggagggct gcgagcgcca gcagcacggg gagggagcgg cgggcgaggg
     6841 tcacttgcgc gagcctgcgc gcggggaacc gtcttgccca ggcgggtgcc agcttcagga
     6901 ccgggcgggg cgttggcgat gggctcgccc gcctccagat ttccgcctcc ggtcctatct
     6961 gttgcaactc gcctcctcca ccatccaagt gacgagcgcc ggcaaactca agccggcatg
     7021 cgccgccagt tccggcacca gggacgtccc cgtcattccc ggttgcgtgt tcacttccag
     7081 gcaagccagc tcacctgtgc cgcccggcgt gtcgtcgtag cgaaagtccg cccgggtcac
     7141 gccccggcac ccgagcgccc ggtgcgccgt cagcgttaac ttctgcacca attcgtaaac
     7201 attcgatgaa atcggcgcgg gcagcaggtg gcgcgagccg ccgtcggcat acttggcctc
     7261 atagttgtag aagcgcagcg cctcggcggg cacgatctcc gtgaccccca aggcgcggtc
     7321 gccgagcacc gcgcaggtca attcgcgccc ggcgatgaac ggctcggcca acagctcctc
     7381 gccgtaggcc cagctcggat cgttcagttc ctggggcggg tgcagatgat cctcacggac
     7441 gatatagacc ccgacgctcg atccctcggc gaccggcttc aagacatagg gtcgcgccag
     7501 cgcgtgcgcg cgcgccgcct caatccggcg cacgacgcgc ccgcccggca ccggcacgcc
     7561 ggcggcctgc agcacggatt tcgcacgctg cttatgcatg gccaaggcag aggccagcac
     7621 gccggaatgc gtatagggca accccaacac ctccaacagg ccctgcacgc aaccatcctc
     7681 accccagcgc ccgtgcaggg cgttgaagca cacctcgggc ctcgcttggc tcagctgttc
     7741 gccgagattg cgctgcgcgt cgatgcgcgt aacgcgatag cccgcctgtt ccagcgcgtc
     7801 ggcgcaggcc ttgcccgagc gcaggctcac ctcgcgctcc gccgagaagc ccccgagcag
     7861 taccgccacg tgctggaatc ggcgccccgg gctggtcatg gtgcaagcgc cttagccaag
     7921 gcctctccga tcggtcgtcc ctcgctggga agcccttggc gaatgatctc ccagtgcaag
     7981 gtgacgccgc agacggccaa aacccgcgcg cgcacggttt cgcccagccg ctcgatgtcc
     8041 tcagctgtgg cggcgccgcc gttgatgaga aagttgcagt gcatctccga caccttggcc
     8101 cccccgacgc tgaggccgcg acagccggcc tggtcgatca gctgccaggc gcgcgccgcg
     8161 gacgggttct tgaaggtgga gccgccggtg cgctccttga tcggctggtg cgcctcgcgg
     8221 tagtcggcga cctcggccat ctcctggaga atctcggccg aactgccggg gcacccctga
     8281 tagagggctt ccgtgaaaat ccaatcgtcg ggcacaccgc aatggcggta gctgaggttg
     8341 aggtcggccg cggtcaagac gtgctggcgc ccttgccgat cgacggcgct ggccgcgcgg
     8401 atgacgtcgc gggtctcgcg gccatgcgcc cctgcattca tgcgcagtgc cccgccaatc
     8461 gaaccgggaa tgccgcgata gaaggctaag ccagcgatgg cggcatcggc ggccgcgcgt
     8521 gccagcttca cgtcgggaac ggcggcgccc acccggaggt gcgaatctgc ttcgacctgt
     8581 gtgtgggcaa agccgcgccc gaggcgaatg acgacgcctg gcacgccgcc gtcgcgcacc
     8641 agcaagttcg agccaagccc caacacgaag gtcgggatcg aggccggcaa gcgggagaga
     8701 aagtaggcga ggtcgtcggc atctgccggg ctgaagagga cctccgccgg cccgccgacc
     8761 cgaaaccagg tgagatcggc caggcggaca tttcccagca ggcggccccg cagcgcgggc
     8821 agctcggcgg caagcctggc gctgaggtcg ggcagcgtca cgcgacacct ccggccggca
     8881 ccggctcggc cgccagccac tgcggcaggg catgcgccca ctccgtgcta tttcccgcac
     8941 ccaagcacac caccgtgtcg ccggcacggg cggcaaatct caacgcggct ggcaggtccc
     9001 gctcgctgct gaccgtcgcg acggaacggt gccccgtcgc gcgcacccgc tcggccagcg
     9061 cggtgtgatc gacgccggcg atgggcagtt cgcccgccga atagagcggc gtcaggatga
     9121 cgctatcggc atccttgaag cagctggcaa actcatcgaa aagatcgcgc acgcgcgtgt
     9181 agcgatgcgg ctcgacgatc gcaatcaccc gccccttggc gccggcgcgc gcggctttca
     9241 gaaccgcggc gatctcgacg gggtgatgac catagtcatc gtagatggcg atgccgttcc
     9301 agctgcccgt tggctggaag cgacgcttga cgcccgcaaa cgaggcgatc gcggccctga
     9361 tggtatcgtc agggatgccc gcttcgcagg cggccgcgat ggcggccaag gcgttgagcg
     9421 cgttgtgctg accggggatc ggtaccgacc agccccgaag cgagcgcgcg ccgccggcga
     9481 cgcggctgcc aagatcggca tcgaacagcg tgtgggcgcc ttcgacccgc gcctgcttca
     9541 agaggaggtc ggccccgggg tgtgtcccat aggtcaacag ccgccgcccg ttgcgccgca
     9601 tttccagctg ctcgagcatg tcgcgcacaa cgggatggtc aatgcaggcc accgccaacc
     9661 cgtagaaggg tatgttgcgc aaaaaggtag cgaattgctg atgcatgttc tcgacggtct
     9721 tgaagtagtc gagatgctcg ggatcgatgt tggtgacaat gccgatttga gtcgggatct
     9781 tgataaaggt accgtcggac tcatcggctt ccacgatcat ccactgaccg tcgccgaggc
     9841 gcgcattgga accccaatcg ttgacgatgc cgccagtgat gaccgtaggg tccttgccgc
     9901 cggcccgaaa cagatgcgcg atgagagagg tcgtcgtggt tttgccgtgc gtgccggtca
     9961 ccgcgacggt ggcgtagagc cgcatgagct cggccagcat ctcggcgcga ccgatgatgg
    10021 gcagcccctt ggcgcgagcc gcctcgagtt cgacgttgcc gggcttcacg gcggtggaga
    10081 cgacgacgaa tttggcaccc accagattga cgacgtcatg gccgacgaac acgcgaatgc
    10141 ccttggcgcg caggcgcttg acgttggcgc tctctttctg gtcgctgcct tgggcaacat
    10201 agcccttggc gtgcaggatc tcggcgatgg cgctcatgcc gataccgccg atgccgacaa
    10261 tatgcacggt gccgatcttg gcaggcatct gcattgaact cttccttcct gccgcccgtt
    10321 tgccgtggtg gctccaagtc ctggcacaag ttacgcaccg tgccttgggt tcccgatcag
    10381 ctcctcgacc aggtccgcca gacgcgcaac ggcatccggc cggccttgcc gcttggctgc
    10441 ggcggccgcc acaaccaaac gcgcgggggc tgccattacc tctgcgaggc tggccgccag
    10501 acgctcaggt gacaagtcac cctgctctat gcaccaggca ccccccgatt cggcaagccg
    10561 catcgcattg agcctttggt cgttatcgat ggcgtgcggc agcggcacaa gaatggcagg
    10621 ccggcccaaa acggcaagtt cggccaccgt ggaggccccg gcgcgggcaa tgaccaggtg
    10681 ggcttgggcc atttcctcgg gcaggttggg aaaaaagctc tcgaggtgtg cgcgcacgcc
    10741 ctgctgctga taggcggcct tgaccccgtc gaggtcttct gcgcggcatt gctggaccac
    10801 gacgagccgg tcccgggcag ccgctggcag gcgcgccaat gccggcggga ggagctcgga
    10861 gaaggcccgc gcgccctggc tgcccccgaa caccaacaga cgaaaggggt cgcgcgcctc
    10921 gggcgcacgg tagggctgat cggcccattg cagcacctgc gcgcgcacgg ggttgccgac
    10981 gtggcgcacc atcgggcgcc ggccgatctt gaggaacttg gtgttctcaa acgaggtggc
    11041 gacggccgtg acgtaggcgg ccaagaaccg gttggcgcga ccgagcacgg cattttgttc
    11101 gtgcaaaagg gtgggtattc cgagcacgcg cgcggccacg accggggcaa aggccggata
    11161 accgccaaaa ccgacgacgg cgtggggtcg aaggcggcga aagaggccga aggcgccggc
    11221 catgccgcgc atgagcgtgg tgccggtctt ggcggccgcg cacaaagagc gtcccgccag
    11281 ggtggcggag ggaaggcgat ggatcgcgcg ggctggaaaa tccacgccat agcgtccacc
    11341 gcgtgcatcg gtgacgagat cgaccgccag gcgacggcga ccgagctcct cggccagcgc
    11401 gaaagccgga aacaaatggc cgccggtgcc ccccgcggcc agaacgaccg acggtcccct
    11461 cacgctcgcc atgaccgcgc cgacccataa gctgccgagg ccatgaaatt gggccgctcg
    11521 aggctggccg ggtcggcgcg ccgccggccg aggcccaaga gcatgccaaa caggagtccg
    11581 acgccaagca tcgaggagcc accactggag atgaagggca gggtgatgcc cttggccggc
    11641 gccaatccaa cgttgaccgc catgttcata ctggcctgca gcacgaagag gagggcgagc
    11701 cccgagcagc tcaggcgaag aaaggcgtcg ccctcgcgca gctgacgcag caagacacgg
    11761 ctgccgatga gagcaaacaa gccaaggatc acaaggcagg ccaagacgcc gtactcttcg
    11821 gcgatgacgg caaaaataaa gtcggtatgc gcgtccggca gagcggtttt tagggtgcct
    11881 tcgccgggac ccttgccaag caagccgccc tccccgaagg agcgtaacgc gcgatcagtt
    11941 tggaaggtat cgccgagcgc gggattaagg aagcgttcga cgcgccagcg cacgtgccca
    12001 aagccgaaat aggcggcaac gagaccggcc cccagcgccg ccaacaccag aaagaaccac
    12061 ttgagtggcc ggttggcgag aaagaacagc gcgctccaga ccaggctgat cagcaaggtt
    12121 tggccgaggt cgggctccag caccaacagg gcggcgaaca gcaaatagag cgccacagcc
    12181 gcgctggtgg ccggcatatc cgggcgccgc tcggcttcgg cgaacagcca ggccgacaga
    12241 atgacgaagg cgggtttggc aaattcgctg ggctggaggg tgtagccgcc gatgtggagc
    12301 caacgccggg ccccgttgat ttcctggccc gtcaacagga cgaggagcat gagggggagg
    12361 ctcgtgccga ggaccagcag ggcgaggcgg cgaagctggg ccggggtcag cagcgagacg
    12421 gcgaacatgg ttgcaagcgc gattgtggca aacagaaaat ggcgctcgac gaagtagaac
    12481 gtcggcaggc cgcgcttcag cgcgatggcg gggctggccg cgagcgacag cagcatcccc
    12541 gtgccggcga tcaccaagac cgtggccagc agcacgcggt cgacgctgaa ccaccattcg
    12601 gcgaggcggc ttctttccgc gcgtgacaag gtcatcggca gcccccttcg cgctctttgc
    12661 ggacccgcgc catcccctta cctttccgct tcgacgcgcg gcagggccgc gacaagggct
    12721 cggaaggcgt cgccgcgccg ctcgaaattg cgaaattgat cgtaggaggc gcaggccggg
    12781 gagagcagca cgatcggttg cgtgccaccg aggcacgccg cgtcacgcgc ggccgccacc
    12841 acggcgactt ccagcgtatc gcagcgctcg aacgccacct tgttgttgag cgtcgcggca
    12901 aaggccggcg tggcttcccc gatcaggaag gccttgcgga tgcgggcaaa atagggctcc
    12961 aggctggtga tgcctccctc cttcggcttg ccgcccagaa tccagaagat atcgcccggg
    13021 aaggcggcga gcgcggttgc ggcgctatcg gcgttggtgg ccttgctgtc gttgataaag
    13081 agcgtggcgc cgatcctgcc gacctcctcg agccgatgcg gcaggccagg gaaggtcggc
    13141 agcgccgcgg cgatttgctc gggccttacg ccaaggtgca tggcggcggc gctggcggcc
    13201 aaggcgttct gggcattgtg gcggccgcgc aaggcgccga cgccggcaag atcggcaaaa
    13261 gcccccaagg gaccggtcca gggcgcgcga gcgatgagct cactgccaag ggcataccaa
    13321 ccatggccga ggcgcgattg cgcggagacg agatcgaccc agctgcgatt ggcgaggcgc
    13381 aagcgttcgg agatgtcctg gcaccaatcg tcatcgtcgc cgacgatggg atgctcggcg
    13441 ctttcgacca cgcgggcctt gagtgccgca tagtgctgca tgctgccatg acggtcaagg
    13501 tgatcgggcg agatgttgag gaggatgccg atgcttggcg caagcgaggg cgtgagctcg
    13561 atctggaaag acgacatttc cacgacgtga acgcactcgg cggccggcgg ggcgaggtcc
    13621 aagatggcgg tgccgatgtt gccgcccagc gccacctcaa ggccggctga ccgcaacagg
    13681 tgggcaagca gcgcactggt cgtggatttg ccgttggtgc cggtgacggc gacgaagggg
    13741 ctcgcgcctc cggcacgcgc ccgctcgcgg cagaagagtt cgatatcgcc gaggatcggc
    13801 acgcccgccg cgcgtgcctt ggccacggac caatgcggct cgggatgggt gaggggaata
    13861 ccgggggcga ggatgagtgc cgcaaagcgc gaccaatcgc tctccgcgag gtcctcgatt
    13921 tcgatctgcg cggccgcgcc cgccgaccgg ctgagcgcgt cgtcgtccca ggccttgagc
    13981 cgcgcgccgc cggccagaag ggcccgggcc gcggccaagc ccgaacgccc gagcccgacg
    14041 agcgcgaggc att
//
LOCUS       DASTMT010000141         1748 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_2047230, whole genome shotgun sequence.
ACCESSION   DASTMT010000141 DASTMT010000000
VERSION     DASTMT010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 1748)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1748)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1748
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2047230"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(360..1658)
                     /gene="phoR"
                     /locus_tag="VFR00_03040"
     CDS             complement(360..1658)
                     /gene="phoR"
                     /locus_tag="VFR00_03040"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HEU0158258.1"
                     /translation="MLRVQDQIARSWQGVREHMASALGRIAARKREVILAALLTTFFL
                     LAGPSSLPALVGLALTVAWAVFWPAEDAQGSFKPRGASAADDRLWRAVVDAVPEPAIV
                     LGDAGQVLHANRMAGDLFGIRRRGGHITTMVRDPELLAAVEQALATRQARVVELHARV
                     PVERRLLMMLAPLDQAGADSAPALLISIRDLTEQDRLARMRADFVANASHELRTPLAS
                     LRGFVETLQGAAKDDPEARDRFLRVMSEQAQRMTRLIDDLLSLSRVEMREHLTPAEQV
                     DLNEAAQHVMQTLQPIAGSAGAKIELRELPTPAIIKGDRDEIVQLLQNLIQNALKYGK
                     AGGRIGVEIAREAQGAGRGTRFRLSVTDDGPGIAPEHLPRLTERFYRVSVAASREKGG
                     TGLGLAIVKHILNRHRGELIITSKLGQGSTFAAVLPAVTD"
BASE COUNT          300 a          585 c          584 g          279 t
ORIGIN      
        1 aaagacgatc gggcacgcaa aggagcactc gaacgccgac agccagaccg gccgacgatc
       61 aacgcgagac tgaggatgac gctgaacttg gaagtactga cgcgtactgc tcttaggcgc
      121 gcacacaacg gtgtgcgact acgcgtactt ggccggccaa cttggctatc gctggtgaac
      181 gcgagcgatc cctggccggc agtccgagaa gattactatg gtgttaacgg ccttctcaag
      241 gccgcgaccg gcgaatcgaa gcgcaagccg ggaagacgtg gcaagctcgc cacacgaggc
      301 cctacaacct gttgacagac gccgcgcgcc acagcccgct cgagcgcacg ggccgcgcct
      361 cagtccgtga ccgccggcag cacggccgcg aaggtcgagc cctggccgag tttggaggtg
      421 atgatgagct cgccacgatg ccggttgagg atgtgcttga cgatggcaag gccgagaccg
      481 gtgccacctt tctcgcggct ggccgccacg ctcacccggt agaagcgctc ggtcaggcgg
      541 ggcaggtgct cgggcgcaat ccccgggcca tcgtccgtga ccgacaggcg gaagcgggtc
      601 ccgcgtcccg cgccctgggc ctcgcgcgca atctccacgc cgatgcgtcc cccggccttg
      661 ccgtacttca aggcgttttg aatgaggttc tgcaggagct ggacgatttc gtcgcggtcg
      721 cccttgatga tggcgggggt gggcagctcc cgcaactcga tctttgcccc cgcgctgccg
      781 gcgatcggct gcagggtttg catcacgtgc tgcgccgcct cgttgaggtc cacttgctcg
      841 gccggcgtca ggtgctcgcg catctccacg cggctgagcg agagcaggtc gtcgatgagg
      901 cgggtcatgc gctgggcttg ctcgctcatc acgcgcagga agcgatcacg tgcttccgga
      961 tcgtccttgg ccgcaccctg caaggtttcg acaaatcccc gcaaggaggc aagtggcgtg
     1021 cgcaactcgt ggctggcatt cgcgacgaaa tcggcgcgca tgcgcgccag tctgtcctgc
     1081 tccgtcagat cgcggatgga aatgaggagg gcgggcgcgc tatccgcgcc ggcctgatcc
     1141 aagggcgcca gcatcatgag cagccggcgc tcgacgggca cccgcgcgtg cagttcgacg
     1201 acccgggctt gccgggtcgc cagggcctgc tccacggcgg ccaacaactc tgggtcacgc
     1261 accatggtcg tgatgtgccc gcctctgcgc cgtatgccga acagatcgcc ggccatccgg
     1321 ttggcatgca gcacctggcc cgcatcgccg agcacgatgg cgggttccgg tacggcgtcg
     1381 acgacggcgc gccaaagccg gtcatcggcc gccgaggcgc cgcgcggctt gaaactgccc
     1441 tgcgcgtcct cggcgggcca gaacacggcc caggcgacgg tcagcgccaa gccgaccagg
     1501 gccggcaggc tcgatggccc cgcgagcaag aaaaaggtgg tgagcagcgc ggccaggatc
     1561 acctcgcgct tgcgcgcggc gatccgccct aaggcggaag ccatgtgctc ccgcacgccc
     1621 tgccagctgc gcgcgatttg gtcctgcact cggagcatgg gacaagttgt tgttgcatgc
     1681 caacctacca tgccctcaac cttgctgcag gctctcgatt gcattgtcca gccgaaatgt
     1741 cggacagt
//
LOCUS       DASTMT010000142         2734 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_1716589, whole genome shotgun sequence.
ACCESSION   DASTMT010000142 DASTMT010000000
VERSION     DASTMT010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2734)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2734)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2734
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1716589"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..941)
                     /locus_tag="VFR00_03045"
     CDS             complement(<1..941)
                     /locus_tag="VFR00_03045"
                     /inference="COORDINATES: protein motif:HMM:NF019285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HEU0158259.1"
                     /translation="MLLQAQSALGIVALPLLAWALSEKRWALPPRRLLRILLAGTLIQ
                     IAIAAIMLNVPASRALFDWAAGLVAALQAATNAGMRLVFGYVAGGPAPFEVTQPQNSF
                     ILGFQALPLILVISALSRLLYHWGALQWVVRSIGRGLQLALGVSAPVGISAAANIFVG
                     MVEAPLLVRPYLQQMSRGGLFATMTVGMAGVAGTVLALYASILEPVLPGAAGHLIVAS
                     VISVPAALMLAELMVPQGAEQDVTEAAADILIDDPPQSSMDAIAQGTREGIELVVNVT
                     AMLIVIVALVALANQLIAVIGRPFGLVATLEQMLGWL"
     gene            complement(1042..1968)
                     /locus_tag="VFR00_03050"
     CDS             complement(1042..1968)
                     /locus_tag="VFR00_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011502047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEU0158260.1"
                     /translation="MGLRRIVAALALLAVAGAGLFYVLTIPKPALTSALPARRADLGN
                     GEIMFNAGGCASCHAIPKQEDTRRLGGGLALNTPFGRFYVPNLSSDATHGIGAWSEEA
                     FVNAMLRGVGRHGEHLYPAFPFTSYQRMALDDVRDLFAFLRTLPAETNPNHPHELAFP
                     FNIRRGLGLWKLIYLDGKGFVPDAAKSARLNRGAYLVEGPSHCAECHSGRDLLGGIIP
                     ERRFAGGPDPEGKGWIPNITPGDNGIAAWSEKDIAYLLETGTTPDYDSVGSSMADVVV
                     NTGKLPAQDREAMAAYLKTLPPRTGKAPAKKP"
     gene            complement(2090..2533)
                     /locus_tag="VFR00_03055"
     CDS             complement(2090..2533)
                     /locus_tag="VFR00_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018391381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEU0158261.1"
                     /translation="MLRVVAAAAALAIGATVVLAQNAAVIDQRKQAMKAFGGAAKSGG
                     AMAKGEAPFDLAKAQESLKTIQAKAGEAKNLFPDDSKDGDTAALPEAFANKADLMTKF
                     DKMAADAKAAEAAVKDEASFKAEWPKVTSNCGGCHKAYRKPPPQK"
BASE COUNT          493 a          872 c          913 g          456 t
ORIGIN      
        1 aacagccagc ccaacatctg ttcgagcgtt gcgacgagcc cgaaggggcg gccgatcacc
       61 gcgatcaact gattggcaag cgcgaccaga gcgacgatga cgatcaacat cgccgtgacg
      121 ttgaccacga gctcgatgcc ctcgcgggtg ccctgtgcga tcgcgtccat gctggactgc
      181 ggcgggtcat cgatcaggat atcggcggcg gcctccgtta cgtcctgctc ggcaccttgc
      241 ggcaccatca gctccgccaa catcagggcg gccggcacgc tgataaccga ggcgacgatg
      301 agatggccgg cggcgccggg caggacgggt tccagaatag aggcataaag agcaagcacc
      361 gtgcctgcga cgcccgccat gccgacggtc atggtggcaa acaggccgcc gcggctcatc
      421 tgttgcaggt aggggcgcac caacagaggc gcctcgacca tgcccacgaa gatgttggcg
      481 gcggctgaga tgccaacggg cgcgctgacg ccaagggcca gctgcaggcc gcggccaatg
      541 gaacgcacca cccactgcaa ggcaccccaa tggtagagga ggcgcgaaag cgcgctgatg
      601 acgaggatca gcggcaacgc ctggaagcca aggataaagc tgttctgcgg ctgcgtgacc
      661 tcgaagggcg ccgggccgcc ggcgacgtag ccgaagacca gtcgcatgcc cgcgttggtg
      721 gccgcttgca aggctgcgac caacccggcc gcccagtcga agagcgcacg cgaggccggc
      781 acgttgagca tgatggcggc gattgcgatc tggatcagcg tgccggcgag taggatgcgc
      841 agcagccgac gcgggggcag cgcccaacgt ttttccgaca gcgcccacgc cagcagtggg
      901 agcgccacga tgccaagtgc gctttgtgcc tgcagcagca tgaggcggcc ctcgctggcc
      961 tgaagccgat tgctgagcac gagcctgcac tattgccacg accgcggcca gcacgctgtc
     1021 aacggcgctt gcgggcagcc gttagggctt tttggcgggt gccttgcccg tccgcggcgg
     1081 cagggtcttc aaataggccg ccatggcctc gcgatcctgc gccggcaact tgccggtgtt
     1141 gaccaccacg tcggccatgc tcgagccaac cgaatcatag tccggcgtgg tgccggtctc
     1201 gagcaggtag gcgatgtcct tttccgacca ggcggcaatg ccgttgtcgc cgggtgtgat
     1261 gttggggatc cagcccttgc cttcgggatc gggcccaccc gcgaagcgcc gctcggggat
     1321 gatgccaccg aggagatcgc gaccgctgtg gcattccgcg cagtgcgacg gtccctcgac
     1381 gagataggcg ccgcgattga ggcgggcgct cttcgccgcg tcgggcacga agcccttgcc
     1441 gtcgagatag atgagcttcc acaggcccaa gccgcgccgg atgttgaatg ggaacgccag
     1501 ctcgtgcggg tggttggggt tcgtttcggc cggcagcgtg cgcagaaagg cgaacaggtc
     1561 gcgcacatcg tcgagcgcca tgcgctgata ggacgtaaaa gggaaagctg gatagagatg
     1621 ttcgccgtgc cgcccgaccc cgcgcagcat ggcattgacg aaggcctcct cggaccaggc
     1681 accgatgccg tgcgtcgcgt ccgatgagag gttgggcaca taaaagcgcc cgaacggcgt
     1741 gttgagcgcg agcccgccgc cgaggcggcg ggtgtcttcc tgtttcggga tggcatggca
     1801 ggaggcgcag ccgccggcat tgaacatgat ctcgccgttg ccgagatcgg cgcggcgtgc
     1861 cggcaaggcg ctggttagcg cgggcttggg gatggtgagc acataaaaca gaccggcacc
     1921 ggcaaccgcc agcagcgcca acgccgcgac gatcctcctc aagcccatag agcctccatt
     1981 ggtgccaagc aaacgggcac caccatacat gaaaagcgcg cgaggccggc tcgcgcgctt
     2041 ctggtctgaa accgtagcaa ccggggaaac cctccggata tcgcccggcc tacttttgtg
     2101 gcgggggttt gcggtaggcc ttgtggcagc cgccgcagtt gctcgtcact ttcggccact
     2161 cggccttgaa gcttgcctca tccttcaccg ccgcctcggc ggccttggcg tcggcggcca
     2221 ttttgtcgaa ctttgtcatc aggtcggcct tgttggcgaa ggcttccgga agggcagccg
     2281 tatcgccgtc cttcgaatcg tcggggaaca ggttcttggc ctcgccggcc ttggcctgga
     2341 tcgtttttag cgactcctgc gccttggcaa gatcgaaggg tgcctcaccc ttggccatgg
     2401 caccgccgct cttggcggcg cccccgaaag ctttcatggc ctgcttgcgc tgatcgatca
     2461 cggcagcatt ttgggccaac acgacggttg ccccaatggc gagggccgcg gcagcggcta
     2521 caacgcgtag catgtagact cctcccgaga attgccggcg cggcaacctc aaccctcgag
     2581 gagtctccgc ggcccgcccg ttgcgcacga agatggcttc gtgcaaacgc caactttacc
     2641 gaggcgacgc gcggtttcaa cgcccccacc cgcactttat tttgattggt gcgccggaat
     2701 aaattggagg tcaggatcgt ctgctggcat aagt
//
LOCUS       DASTMT010000143         3231 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_4634997, whole genome shotgun sequence.
ACCESSION   DASTMT010000143 DASTMT010000000
VERSION     DASTMT010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3231
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4634997"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..245)
                     /locus_tag="VFR00_03060"
     CDS             complement(<1..245)
                     /locus_tag="VFR00_03060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HEU0158262.1"
                     /translation="MRRPNTKDLLVGLLFLAFGSAFLLLAQGYPLGSARRMGPAYFPQ
                     LLALLLMAIGLLAMLRALASAPTGIGRVAAKPLLMVT"
     gene            complement(371..1405)
                     /locus_tag="VFR00_03065"
     CDS             complement(371..1405)
                     /locus_tag="VFR00_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018646089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HEU0158263.1"
                     /translation="MDAAGLGLLFGLIAVALLFDFLNGLHDAANSIATIVSTRVLPPR
                     AAVFWAAFFNFIAFLFFGLHVAQTLGTGLIDPAIVTPRVIFAALTAAIVWNLVTWWAG
                     IPSSSSHALIGGLIGAGLASAGLGAIVWTGVLKTVGAIVISPAVGFALALVFVAIVSW
                     IFVAKTPYAVDRLFRRLQFVSAALYSLGHGSNDAQKTMGIIAGLLFAQGHLGREFYVP
                     FWVVLSCQAAMALGTLFGGWRIVKTMGSRITRLTPMQGFCAETGGALTLFVATELGIP
                     VSTTHTITGAIVGVGAARRVSAVRWDVASDVVTAWVLTLPAAAAVAALTYAAVGLFAW
                     GCRGSIAACL"
     gene            complement(1424..2068)
                     /locus_tag="VFR00_03070"
     CDS             complement(1424..2068)
                     /locus_tag="VFR00_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007592082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HEU0158264.1"
                     /translation="MLDWFQRLLPREEKFFPLFERHAACIAAAAEALRRMLDGGDAVP
                     DHCRLVMRHEEEADGITREVMIGVRSTFITPFDRGDIKDLITAMDDAIDQMQKTAKAI
                     VLFELLSFAPQMRSVADSIAECSQLVRRAMPLLANIGQHAGQLTEICQQIGRIEGRAD
                     EVYDQGLKQLYLQAKGGDAMEFVRCNEIYDHLEKSVDRFDDIANQIQGIVIEHV"
     gene            2342..>3231
                     /locus_tag="VFR00_03075"
     CDS             2342..>3231
                     /locus_tag="VFR00_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEU0158265.1"
                     /translation="MTQMQPRNSDLDSAVAEARERYSAARPESERIHRAARAVLPGGN
                     TRSVLFYTPFPTAMARGAGCSLEDVDGRRYLDFCGEYTAGLFGHSEPRIQAALHAAID
                     RGLNLAAVGEREAQLAALLCARFPSLERVRFTNSGTEANMLALGVARAYSGRPGVLAF
                     RGGYHGGLLTLVGGGSAINSPIPLTLADYNDTQQTLEVLRAHAGELAAVILEPMMGSG
                     GCIPAHPEFLRALREATRELGVLLLFDEVMTSRHSSGGLQKLRGVTPDLTTLGKYMAG
                     GMSFGAFGGRADVMGLFDGHR"
BASE COUNT          607 a         1046 c         1073 g          505 t
ORIGIN      
        1 aaggtcacca tcagcagcgg cttggccgcg acccgaccga tgccggtggg ggctgaggcc
       61 aaagcgcgca gcatggcgag aaggccgata gccatgagca agagcgccag cagctgggga
      121 aagtaggcgg ggcccatgcg ccgggcgctg ccgaggggat agccttgagc caacaggagg
      181 aatgccgagc cgaaggccag aaagagcaat ccgaccaaga gatctttggt gttcggccgg
      241 cgcatgacag gggctcgaaa aggctgcaag ccagcttagc cgatcccgcg ggccgctgtc
      301 cgttgcggcg ccggctgggt tcgccggccg ctggccgagc gcggcaagga aggcacacgc
      361 gcgggcaaga tcacaggcac gcggcgatgc tgccgcggca accccaagcg aaaagcccca
      421 cagcggcgta ggtcagggcg gcgaccgcgg cggcggcggg cagggtcaag acccaggcgg
      481 tgaccacatc gctggcgaca tcccagcgca cggccgagac gcggcgggcg gcgcccacgc
      541 ccacgatcgc cccggtgatg gtatgggtcg tcgagacggg aatgccgagc tcggtggcga
      601 caaacagcgt gagcgcgccg cccgtttccg cgcaaaaccc ttgcatgggt gtgagacgcg
      661 taattctgga gcccattgtc ttcacaatgc gccagccgcc gaacaaggtg cccaaagcca
      721 tggccgcctg acagctgagg accacccaga acggcacgta gaactcgcga cccaggtgcc
      781 cctgggcgaa cagcagtccg gcaatgatgc ccatggtctt ttgcgcatcg ttgctgccgt
      841 ggcccaggga atagagcgcc gcggagacga actggagccg ccggaacagg cggtcgaccg
      901 catagggcgt cttggcgacg aagatccaag agacgatcgc cacgaacacg agcgccaagg
      961 cgaagccgac ggccggcgag atgacaatgg ccccgaccgt cttcagcacg cctgtccaca
     1021 cgatggcgcc gaggccggcg ctcgccagcc cggccccaat cagcccgccg atcagcgcat
     1081 gcgagctgct cgacgggatc ccagcccacc aggtgaccaa attccagacg atcgccgccg
     1141 tcaaggcggc aaagatcacg cgcggcgtca cgatggctgg atcgatgaga ccggtgccga
     1201 gcgtttgcgc cacatgcagg ccgaagaaca agaaggcaat gaaattgaag aacgcggccc
     1261 aaaacacggc cgcccgcggt ggcaacaccc gggtggagac gatggtggcg atggagttgg
     1321 cggcgtcatg caggccgttg agaaaatcga acagcagcgc cacggcgatc aggccgaaca
     1381 atagacctaa gccggccgca tccatctgcc ctcctcgctc ggcctagacg tgttcgatga
     1441 cgataccttg tatctggttg gcgatgtcat cgaagcggtc gacgcttttc tccaggtgat
     1501 cgtagatctc gttgcagcgc acgaattcca tggcgtcgcc gccctttgcc tgcagataga
     1561 gctgcttcag gccctgatcg tagacctcat cggcccgtcc ctcgatgcgg ccgatttgct
     1621 gacagatttc ggtcaactgg ccggcgtgct ggccgatgtt ggccaacagc ggcatggcgc
     1681 ggcgcaccaa ttgggagcac tccgcgatgc tatcggcgac actgcgcatc tgcggcgcaa
     1741 agctcagcaa ttcaaagagt acgatggcct ttgccgtctt ctgcatctga tcgatggcat
     1801 catccatcgc ggtgatcaga tccttgatgt caccgcgatc gaagggggta atgaaggtcg
     1861 agcgcacgcc gatcatgacc tcgcgggtga tgccgtcggc ttcttcctcg tggcgcatca
     1921 ccaggcgaca gtggtccggc acggcatcgc cgccgtcgag catgcgacgg agcgcctcgg
     1981 ccgccgcggc aatgcacgcg gcgtgtcgtt cgaacagcgg aaagaacttc tcctcccggg
     2041 gcagcagccg ttgaaaccag tcaagcatcg ccgaacgctc gctcgcgcta gcgtgcaagc
     2101 ctacgcgggt ccgctgcgcg cgcctacttt tttgtgcttg gcttgcgacg aagacgggaa
     2161 gcgcagccgg cgcggcggcg ccgaccggcg cgcctgcgac gcgccagcaa ggcaaagacg
     2221 aggtatcgcc tgcagctggc atcgtgcaca atgccagcac aagcgccgcc tttgcccgcc
     2281 accgcaagga cggtaccgtt gcagcattgg tcgccgcctg cggccaaaga ggatctgcgc
     2341 catgacccaa atgcaaccgc gcaacagcga cctcgacagc gccgtggcag aggcgcgcga
     2401 gcgctatagt gcggcccgcc cagagagcga acgcatccat cgggcggcac gtgccgtcct
     2461 gccgggcggc aatacccgct cggtgctgtt ctacacgcca tttccgacgg cgatggcccg
     2521 cggtgcaggc tgttcgctgg aggacgtgga cggccgccgc tatctcgatt tctgtggcga
     2581 gtacacggcc ggcttgttcg ggcattcgga gccgcgcatc caggcggcct tgcatgctgc
     2641 gatcgatcgg ggactcaatt tggccgcggt cggcgagcgc gaagctcagc tggcagcgtt
     2701 gctgtgcgcg cgctttccat cgctcgagcg ggtgcgcttc accaacagcg gcacggaagc
     2761 caacatgctg gcccttggtg tcgcgcgtgc ctattccggg cgtccgggag ttcttgcctt
     2821 tcgcggcggc taccacggcg ggctgctgac cctggtgggc ggcggcagtg cgatcaattc
     2881 gcccattccc ctcacgctcg ccgattacaa cgacacgcaa cagacgctcg aggtccttcg
     2941 cgcccatgcc ggtgaacttg ccgccgtcat cctcgagccg atgatgggtt cgggaggctg
     3001 catccccgcc catcccgagt ttttgcgggc cctgcgcgag gcaacccgcg aactcggcgt
     3061 cttgttgctg ttcgacgagg tgatgaccag ccggcattcc tccggcggcc tgcaaaagct
     3121 gcggggggtg acgccggatc tgaccacgct cggcaagtat atggccggcg gcatgagctt
     3181 cggtgccttt ggcggccgcg ccgatgtgat gggccttttc gatggccatc g
//
LOCUS       DASTMT010000144         4359 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_2048535, whole genome shotgun sequence.
ACCESSION   DASTMT010000144 DASTMT010000000
VERSION     DASTMT010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4359)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4359)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4359
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2048535"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..155)
                     /locus_tag="VFR00_03080"
     CDS             complement(<1..155)
                     /locus_tag="VFR00_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018099719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU0158266.1"
                     /translation="MTEARASRVAVLATQGLSRNFGGLAAVRDVSIALHHGELHAVIG
                     PNGAGKTT"
     gene            complement(148..1182)
                     /locus_tag="VFR00_03085"
     CDS             complement(148..1182)
                     /locus_tag="VFR00_03085"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEU0158267.1"
                     /translation="MSARPTPQTWRAKLAGGPSGGWLMGLGTALLVVAPLGLSEFYTG
                     LITSALIAAMLALSLQLLVGCTGLVSLGHAAFYGLGAYTVFAITPAEHARSVLLTLPA
                     AAAVAGLAALLVGALSLRTKGFFFLMVTLAFGQMVFFLFHNTKLGGGADGAYLMRPTL
                     SLGDWQLSLTRRQRPLAYYYLSLLLLVAMYLSLRVLLRSLFGRVLEGIRINEQRMLAL
                     GFNTYRYKLGAFALAGLLAGIAGHMYAMKEGFVNPELVGWHRSAEALLMILLGGLSTL
                     SGPIFGALAYTAIGEIAQLLTERKLLVEGLIILLAVLSLPKGLSGIGLGWRKDGARPR
                     PPAAEAKAHD"
     gene            complement(1179..2048)
                     /locus_tag="VFR00_03090"
     CDS             complement(1179..2048)
                     /locus_tag="VFR00_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019400155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEU0158268.1"
                     /translation="MDFPGLLAQLLNGLQYGLLLFLIASGLTLIFGVMGVINLAHGSM
                     FMIGAYVAYLITARWGSLWLALPAAALGGLLIGAALESGLFRHFYAREHLDQVLLTFA
                     LILLFEEFRSLLVGNDFHSVPVPRILDFSVPITAQFSYSAYRFAVIVICLLLAALLFW
                     WIERTRFGAIIRAAAEKPEMVDVLGINARRVHLTVFAVGTALALVAGALAAPLYAVYP
                     NMGDGFLIISFVVVVIGGLGSVSGAFWSALILGLVDTLGAAYAPKVAGLVVYVLMAAV
                     LLWRPAGLFGRRA"
     gene            complement(2120..3313)
                     /locus_tag="VFR00_03095"
     CDS             complement(2120..3313)
                     /locus_tag="VFR00_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002714200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0158269.1"
                     /translation="MQRPVRPTLSRRGVIGGGAACAAAAGLPSGWAIAQAKPLKVGLM
                     LPYSGTFAKLGENITYAIELFLAEKGGKLGGREIQIIKLDDESKPENAPQNAERLVKR
                     DAVDVLIGTVHSGVQMGIHKVVQESGTLTIVPNAGNVAVTRQLCTKNVFRSSFTNWQP
                     GYGMGLAAAKKGYKKGVWVTWDYAAGAESGAGFKEGFEKGGGEVLPSLALPFPQTNFQ
                     PLLAQIPSLGVDVVGSFFAGGGAVQFVKEYAAAGLKLPLCGSGFLTEGTLDAQGAAAD
                     GVETALHYGDGLDNPKNAAFRKAFKDKAGREADVYAVQGYDAAQLLAVGLEAAKGNVE
                     DEATLYKALRAAKLESPRGPIAMSPSQNITQNIYLRRAEGGQNKVIGIAAENLADPGT
                     GCKLA"
     gene            3803..>4359
                     /locus_tag="VFR00_03100"
     CDS             3803..>4359
                     /locus_tag="VFR00_03100"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU0158270.1"
                     /translation="MRDKIRVAVIDPYPLFREGIIQTIVQSRQLELVAEGATLGEAER
                     AVREQRPDVLIFDIGLPDTGTEGVQDFIKSGPDCKLVVLTSSDDVASVAKALAAGIQG
                     YILRGVTGSELIEAIETIDGGQPYITPKLASRLLTEARGGPLSAIREPTPKLDLSYRE
                     QQVLIRISRGLTNKEIANQLGLNERT"
BASE COUNT          858 a         1383 c         1450 g          668 t
ORIGIN      
        1 gtcgtcttgc ccgccccgtt ggggccaatg acggcgtgca gctcgccgtg atgcaaggcg
       61 atggaaacat cgcgcacggc agcgaggccg ccgaagttgc ggctaagccc ttgcgtggcc
      121 agcaccgcga cccggctggc acgcgcttca gtcatgcgcc ttggcctcgg ccgcaggcgg
      181 ccgcgggcgc gcgccgtctt tgcgccagcc gagcccgatg ccgctcaagc ccttgggaag
      241 cgacaagacc gccagcagga tgatcaggcc ctcaaccaag agtttgcgct cggtgaggag
      301 ctgggcgatc tcgccgatcg cggtataggc cagcgcgccg aaaatcggac cgctgagcgt
      361 gcttagaccc cccagcagga tcatcagtag cgcttcggct gagcggtgcc agccgaccag
      421 ctccgggttc acgaaacctt ccttcatcgc atacatgtgg ccggcgatgc cggcgagcag
      481 cccagcgagg gcgaaggcgc caagcttgta gcgataggtg ttgaagccga gcgccagcat
      541 gcgctgctcg ttgatgcgga tcccttcaag cacgcgaccg aacagtgagc gcaagaggac
      601 gcgaaggctg agatacattg ccaccagcaa cagcaaagac aaatagtagt aggcgagcgg
      661 ccgttggcgc cgtgtcaggg acaattgcca atcgccgagc gatagggtcg gccgcatgag
      721 gtaggcgcca tcggcgccgc cgccaagctt ggtattgtga aagaggaaga acaccatctg
      781 gccaaaggcg agtgtgacca tcagaaagaa gaagcccttg gttctgagcg acagggcacc
      841 gaccaagagg gcggcaaggc cggccacggc ggcagcggca gggagcgtca ggagcacgga
      901 gcgtgcgtgc tcggcgggcg tgatggcgaa gaccgtatag gcgccaaggc catagaaggc
      961 ggcatgaccc aggctgacga ggcccgtgca gccgaccagc agctgcaggc tcaaggccag
     1021 catggccgca atcagggccg aggtgataag gccggtgtag aactcggaga ggccgagcgg
     1081 ggccacgacg aggagggcgg tgcccaagcc catgagccag ccgccgcttg gcccgcccgc
     1141 cagcttggcg cgccaagtct gaggcgtcgg gcgcgcgctc atgcccgccg tccaaacagg
     1201 cccgcggggc gccacagcag cacggccgcc atcagcacat agacgacaag ccccgccacc
     1261 tttggggcat aggccgcacc cagcgtatcg accagcccca ggatcagggc cgaccagaag
     1321 gcgcccgata ccgagccgag gccgccaatg acgaccacga caaaggagat gatcagaaag
     1381 ccatcaccca tgttggggta gacggcataa agcggggcgg cgagcgcgcc ggcgaccagc
     1441 gccagcgcag tgccgacggc gaacacggtc agatgcacgc ggcgcgcatt gatgccaagc
     1501 acgtcgacca tctcgggttt ctcggcggcg gcgcggatga tggcgccgaa gcgcgtacgc
     1561 tcgatccacc agaacagcag agcggccagc aaaaggcaga tcacgattac ggcgaagcgg
     1621 taggcagagt aactgaactg tgcggtgatc ggcacggaaa agtccaggat ccggggtacc
     1681 ggcacggaat ggaagtcgtt gccgaccagc agggagcgga actcctcgaa cagcaaaatc
     1741 agggcgaagg tgagcaggac ctgatcaagg tgttcgcgcg cgtagaagtg acgaaagagg
     1801 ccgctctcga gcgccgcacc gatgaggagg ccgcccagcg cggcggccgg gagcgccagc
     1861 cagagcgagc cccagcgcgc cgtgatcaga taagcaacgt aggcgccgat catgaacatc
     1921 gagccgtgcg ccagattgat gacgcccatg accccgaaaa tgagggtgag cccgctggcg
     1981 atcaaaaaca ggagaagccc gtattgcagg ccattgagga gctgcgccag cagtcctggg
     2041 aaatccatgc cattcagccg cgggccaagg ttaaaacggc cggcccgcca ttgtggcgga
     2101 ccgggccatg gtctcgagct caggccagct tgcagcccgt gccgggatcc gccaggtttt
     2161 cggcggcaat gccgatcacc ttgttctggc cgccttcggc gcggcgcaag taaatattct
     2221 gggtgatgtt ttgcgatggc gacatcgcaa tgggaccgcg cgggctctcg agcttggctg
     2281 cgcgcaaggc cttatagaga gtggcctcgt cctcgacgtt gcccttggcg gcctcgagcc
     2341 cgacggcgag cagctgggcg gcatcatagc cctgtacggc atagacatcg gcctcacgcc
     2401 cggctttgtc cttgaaggcc ttgcggaagg cggcgttctt tgggttatcg aggccgtcgc
     2461 catagtgcag cgcggtctcc acgccgtcgg ccgccgctcc ctgggcatcg agcgttccct
     2521 cggtcaaaaa gcccgagccg cacagcggta gcttgaggcc ggccgccgcg tactccttga
     2581 cgaactggac ggcaccgccg cccgcgaaaa aggagcccac cacgtcgaca ccgaggcttg
     2641 ggatctgcgc caacagcggt tggaaattgg tttgtgggaa ggggagtgcc aagctcggca
     2701 gcacctcccc gccacccttt tcgaagcctt ccttgaagcc ggcgccgctc tccgcgccgg
     2761 ccgcgtagtc ccaggtcacc catacccctt tcttgtagcc cttcttggcg gcggcgagac
     2821 ccatgccgta gccgggttgc caattcgtaa aggacgaccg gaacacattc ttggtgcaga
     2881 gctggcgcgt caccgcgacg ttgccggcgt tgggcacaat ggtcagcgtg ccgctctcct
     2941 gcacgacctt gtggatgccc atttgcacgc ccgagtgtac ggtgccgatc aagacgtcga
     3001 cggcgtcgcg cttgaccagg cgctcggcat tctgcggcgc gttctcgggt tttgattcat
     3061 cgtcgagctt gatgatctgg atctcgcggc cgccaagctt gccgcccttc tcggcgagga
     3121 agagttcgat ggcataggtg atgttttcgc ccagcttggc gaaggtaccg gaatagggca
     3181 gcatcaggcc gaccttcagg ggcttggcct gcgcgatcgc ccagccgctg ggcagccccg
     3241 cagcggcggc acaggcggcg ccacccccga tcacgcctcg gcgcgagagc gttggtctca
     3301 caggtctttg catggcgtcc tccctgccct gtcgggcccc gactttgagc gctgcggttg
     3361 cggtttatgc tggcccttgg ctcgcatacc gggcccgcaa ttgcaacgcc gcagcgctgc
     3421 cgcgccggcg gccacaacgg ctgcgaactg ccgcccagac caagccgagg ggctgggtca
     3481 ggcgtaccct ctgagtgccg ggaaaccccc gcagcggcga acaggcgcaa aacctcggcc
     3541 ttggcccgcg ccagcgcgca tgccgacagg gcacggccct ctaggcgctg aggcccaacc
     3601 aaactttagg ccaacaatat tttgcacttt agtccagtat aaggtacgac ttcctcatct
     3661 cttcgggcgc ccgaagggag ggtagaaagg attgccgtgc aggccggtcc agcggcggac
     3721 ctggtcctgc gaccgcggcg cggcggagcg gtcctgggcc gaggtggctg ccgtggctag
     3781 ggcaaggtgt tggggactgt tggtgagaga caagatccgc gttgccgtca tcgatcccta
     3841 ccccctgttc cgcgagggga tcattcaaac catcgtgcaa tcgcgccaac tcgagctagt
     3901 ggccgagggg gcaacgcttg gcgaggccga gcgcgcggtg cgcgagcagc gccccgacgt
     3961 gctgattttc gacattggcc tgcccgatac cggcaccgaa ggcgtccagg acttcatcaa
     4021 aagcggaccc gattgcaaat tggtcgtgct gacgtcgtcc gatgacgtcg ccagcgtcgc
     4081 caaggcatta gccgctggca tccaaggcta catcttaagg ggcgtaaccg gcagcgagct
     4141 gatcgaagcg atcgagacca ttgatggcgg acagccctac ataacgccaa aactggcctc
     4201 gcgcctgctg acggaggcgc ggggcggacc tttgtcggcc attcgcgagc ccacaccgaa
     4261 gctcgacttg agctaccgtg agcaacaagt actcatccgg atttcccggg ggttgacgaa
     4321 caaggagatc gccaaccagc tcggcttgaa cgagcgcac
//
LOCUS       DASTMT010000145         5954 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_3032097, whole genome shotgun sequence.
ACCESSION   DASTMT010000145 DASTMT010000000
VERSION     DASTMT010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 5954)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5954)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5954
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3032097"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..339)
                     /locus_tag="VFR00_03105"
     CDS             complement(<1..339)
                     /locus_tag="VFR00_03105"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEU0158271.1"
                     /translation="MGMTQARLSRRHLGKAALALALLPRSLAAQKLSDRPIIIIVPFT
                     AGTGIDILARILGEELQQRWGQPVTIENRPGASGNIGSQYAASRAPDGHSLMMTVNTF
                     VMNVSLFKSVP"
     gene            complement(423..1670)
                     /locus_tag="VFR00_03110"
     CDS             complement(423..1670)
                     /locus_tag="VFR00_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018425826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin-dependent oxidoreductase"
                     /protein_id="HEU0158272.1"
                     /translation="MPASKDVLIIGAGIGGLTLALALAKRGIGARLFEAAPELKPIGV
                     GLNLLPHAVAELAALDLLALLAQRAVITREAVFFNRFGQLIHREPAGQHAGHRQPQLS
                     LHRGDLQAVLLEAVAERLGRERINFGWRCTAALEAGELTRAEFLDATGGRLPPQEGAI
                     LVACDGIHSAIRRQLYAAEGEPVYSGVNMWRGVTRQAPFLTGASMVRAGWLTTGKMVI
                     YPIRRYADGSQLVNWVAELETPHYRRWDWTRPGRLEDFIAAFADWHFDWLDVPALIRG
                     AESILEFPMVDRDPLPRWSFGRLTLLGDAAHPMLPRGSNGAVQAILDARALADRLAED
                     DDWVRALANYDQTRRVATTAVVLANRSHPPDAILREIYLRTGDRPFGKIDDVISATEL
                     GQISERYKRISGYARGQGTVAEA"
     gene            complement(1797..2840)
                     /gene="gtdA"
                     /locus_tag="VFR00_03115"
     CDS             complement(1797..2840)
                     /gene="gtdA"
                     /locus_tag="VFR00_03115"
                     /EC_number="1.13.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006611691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047922 - gentisate 1,2-dioxygenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gentisate 1,2-dioxygenase"
                     /protein_id="HEU0158273.1"
                     /translation="MQHIPVETPERKAFYDRIDKENMTALWTVMGALITPEPKSACVP
                     AAWRFAAIRAAMLEAGALITAKEAERRVLVLENPGLRGQSKITTSLYAGVQLVMPHEV
                     APAHRHSQSALRFVLEGKGAYTTVNGERTLMHPGDFIMTPYMAGHDHGNDTDEPMFWL
                     DGLDIPLIQFLDASFVERLEEEVQPVGKPAGDSLARYGANLLPVDHKPNGATSPIFNY
                     PYERTREALYRLSRSADYDPCHGIKMRYTNPATGDHAMPTMATFIQALPRGFRTSRYR
                     STDASVFVAIEGSGRTRVGDTTLEWGPKDIFVVPSWRPVVHEAASESILFSFSDRPVQ
                     ERIGVWREDRGNA"
     gene            complement(2944..4257)
                     /locus_tag="VFR00_03120"
     CDS             complement(2944..4257)
                     /locus_tag="VFR00_03120"
                     /inference="COORDINATES: protein motif:HMM:NF018597.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extensin family protein"
                     /protein_id="HEU0158274.1"
                     /translation="MHYRAKYAETYADLAAWTQARSAAAVRAAANALAQLSTKLSVVT
                     VLGIGSIAILPQQAASTLRPARLAQSPPPIEMVRPKQVIIDEPAAAVELIASRRQRRR
                     AAEPDPPAPVRRNSSIAVEAEPTPAPGTASAPAPAAAPEAPAQSAQGEPPKPDVWSEA
                     EVIGALRDCVKELAPIVAEVEVVAPVKQDQCGTPAPVMLRRVAAGNTRVEIKPPAMVN
                     CAMVAHLHEWVTTVLQPAAEQAFGSPITRLANASGYACRQRNGSQSHSERLSEHALAN
                     AIDIAGFVTADGRTIEVARHWGPTLREQKEAQRSAAQRGRDGKETAKTEAAKAPVRTP
                     SAISRQGGDRLRVAEGGTASGREGATQQRLGRGVEGERDGKAAPQPSEAREVAQASAE
                     GSFLRRLHKGACGIFGTVLGPEANEAHRDHFHLDLAPRRRSAFCE"
     gene            complement(4362..5492)
                     /locus_tag="VFR00_03125"
     CDS             complement(4362..5492)
                     /locus_tag="VFR00_03125"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HEU0158275.1"
                     /translation="MRALAGTLVVSLDQAVAAPMAARRLADAGARVIKLERPEGDFAR
                     EYDKAVHGECTHFVWLNRGKESVVVDLRRGEDRRLFEALLAKADVFLQNLKPGALAKL
                     GYAVEALRQRYPRLIVCSISGYGEEGPYRERKAYDLLVQAESGLASLTGGPEAPARVG
                     VSLVDIGTGLHAYEAILEALIQRARTGEGADIRVAMFDAMMEWMTVPLLFAEYAEAPR
                     RSGLTHPSLAPYGVFTTADAVPILISIQNDREWVSLCSKVLGRPELGTDPRFATSPAR
                     LAHRSQTDGAVAAHFASLDVLTLSKKLEAADIAFARVNEVGDIIRHPHLRRISVDTPS
                     GPVLLPAPPARTGAAPAAFGAIAPLGAHTELVRQEFLGSNGA"
     gene            complement(5510..>5954)
                     /locus_tag="VFR00_03130"
     CDS             complement(5510..>5954)
                     /locus_tag="VFR00_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004124549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase"
                     /protein_id="HEU0158276.1"
                     /translation="LLSKAKDNNASTALGAFVRFFDASFSLEDVRRIEVSLKVEGEDG
                     FVLNGRSSMREIARDPAALVAQMLGRHHQYPDGAVLFLGTMFAPIEDRDAAGQGFTHK
                     LGDITTIAAPELGSLANRMTLSTEAPPWSFGTAALMRNLARRGLL"
BASE COUNT          929 a         1994 c         2057 g          974 t
ORIGIN      
        1 gggcaccgac ttgaagagac tgacgttcat gacgaaggtg ttcaccgtca tcatcaggct
       61 atgtccgtcg ggagcgcggc tggcggcata ttgcgagccg atattgccgc tggcgccggg
      121 ccggttctcg atcgtgaccg gctggcccca gcgctgctgc agctcctcgc ccaaaatgcg
      181 cgccaggatg tcgatgccgg tgccggcggt gaagggcacg atgatgatga tcgggcggtc
      241 cgacagcttc tgcgccgcga gcgaccgcgg cagcagagcc aaggccagcg ccgccttgcc
      301 gagatgccga cgcgaaagcc gagcctgtgt catgcccatc cccctctcat tgtcgtttac
      361 ccaatcggat tgcggtttgt gccagctaaa ggcacgcgcc ggccaagttg tgctgcgccc
      421 cttcacgcct ccgcgaccgt gccctggccg cgcgcgtagc ccgagatgcg cttgtagcgc
      481 tcggaaatct gcccgagctc ggtggcactg atgacgtcgt cgattttgcc gaacgggcga
      541 tcgcccgtgc gcagatagat ctcgcgcaag atggcatcgg ggggatggct gcggttggcg
      601 agaacgacgg cggtcgtcgc caccctgcgc gtttgatcat aattggcgag cgcgcgcacc
      661 caatcgtcgt cctcggcaag ccgatcggcg agcgcgcgcg catcgagaat ggcttgcacg
      721 gcgccgttgg atccacgcgg cagcatcggg tgggctgcat cacccaacag cgtcaggcgc
      781 ccgaagctcc agcgcggcag cggatcgcgg tcgaccatgg gaaattcgag gatcgattcg
      841 gcgccgcgga tcagcgcggg cacgtcgagc caatcgaaat gccaatcggc aaaggcggcg
      901 atgaagtcct cgagccgccc cggccgcgtc cagtcccagc gccggtaatg gggggtctcc
      961 agttcggcca cccaattgac gagctgactg ccgtcggcgt agcggcggat cgggtagatc
     1021 accatcttgc cggtggtgag ccagccggcg cgcaccatgc tggcccccgt caagaatggg
     1081 gcctgccgcg tcaccccgcg ccacatgttg acgccggaat aaacgggctc gccctccgcc
     1141 gcgtagagct ggcggcggat cgccgagtga atgccgtcgc aggcgacgag aattgcgccc
     1201 tcctgtgggg gcagacgccc gccggtcgca tcgaggaact cggcgcgcgt cagctcgccg
     1261 gcttctagcg ctgccgtgca acgccagccg aaattgatgc gctcccgccc gagccgctcc
     1321 gccacggcct cgagcaggac ggcctgcaga tcgccgcgat gcagcgaaag ctgcggctgc
     1381 cggtgaccgg catgctggcc cgccggttcg cgatggatga gctggccgaa gcggttgaag
     1441 aagacggcct cccgcgtgat caccgcgcgc tgggcgagca gggctaacag gtcgagggcg
     1501 gcaagctcgg ccaccgcatg cggcagaaga ttgagcccga cgccgatcgg cttcagctcc
     1561 ggtgccgctt cgaacagccg cgcgccaatg cctcgcttcg ccagggcgag cgccagggtc
     1621 agcccgccaa tgccggcgcc gatgatgaga acgtctttgc tggctggcat ggcgccttgc
     1681 ctttttgccc aatcggcccc gcccgttgtt cggcgtgcgc caggagcacc cctttgcccg
     1741 cttcgcgttg cccttggcgg cgcggccccg cactcggccg gcgccgagag ctcacctcag
     1801 gcgttgccgc gatcctcgcg ccagacgccg atccgctcct gcaccgggcg atcggaaaag
     1861 ctgaaaagta tgctctcgct tgccgcttca tgcaccaccg gccgccagct cggcaccacg
     1921 aaaatgtcct tcgggcccca ctccaaggtg gtgtcgccga cgcgcgtgcg gcccgatccc
     1981 tcgatggcga cgaagaccga tgcatcggtc gagcggtagc ggctggtgcg gaagccgcgc
     2041 ggcaatgcct ggatgaaggt ggccatggtc ggcatggcat gatcgccggt ggcgggattg
     2101 gtgtaacgca ttttgatgcc atgacagggg tcgtagtctg cgctgcgcga cagccgatag
     2161 agcgcctcgc gcgtacgctc gtagggataa ttgaaaatgg gcgaggtggc cccgttcggc
     2221 ttgtggtcga cgggcagcag gttggcgccg tagcgcgcca gcgaatcccc ggccggtttg
     2281 ccgaccggct gcacctcctc ctcgaggcgc tccacgaagg aggcgtctag gaattgaatc
     2341 agcggaatat cgagcccgtc cagccagaac atcggctcgt cggtatcgtt gccgtgatcg
     2401 tgcccggcca tgtagggggt cataataaag tcgccggggt gcatcagcgt gcgctcgccg
     2461 ttgacggtgg tgtaggcgcc cttgccttcg agcacgaagc gcaaagccga ctgcgaatgc
     2521 cggtgcgccg gggcgacctc gtgcggcatg acaagctgga cgccggcata gagcgacgtg
     2581 gtgatcttcg actggccgcg caggccaggg ttctccagca ccagcacgcg ccgttcggcc
     2641 tctttcgccg tgatcagcgc gcccgcctcc agcatggcgg cgcggatggc agcgaagcgc
     2701 caggccgccg gcacacaggc gctcttgggc tccggcgtga tgagcgcacc catcaccgtc
     2761 cacagcgcgg tcatgttctc tttatcgatg cgatcgtaaa aggctttgcg ttccggggtc
     2821 tcgacgggaa tgtgctgcat agctcgctgc ctcggctagg gcgtgcccga cctctcgtgc
     2881 aaggctactt taggcccgcg ccggcgtgcg gtctatgcgc ccgacaatgc cagagcggcc
     2941 ggcctactcg caaaaggcgc tgcgtcggcg cggcgccagg tcgaggtgga agtggtcgcg
     3001 gtgggcttcg ttggcctccg gcccgagcac ggttccgaag atgccacacg cgcccttgtg
     3061 cagccgccgc aggaagctgc cctcggcact ggcctgcgcc acctcgcgcg cctcgctggg
     3121 ctgaggtgcg gccttgccgt cgcgctcgcc ctccacgccg cggccgagac gctgctgcgt
     3181 ggcgccctcg cgtcccgatg ccgtgccgcc ctcggcaacc ctcaagcgat cgccgccctg
     3241 gcgtgagatc gcgctcggcg tgcgcaccgg cgccttggcg gcttcggttt tggcggtctc
     3301 tttgccgtcg cggccgcgct gagccgcgct gcgctgggct tctttttgct cgcgcagcgt
     3361 cggtccccaa tgccgtgcca cctcgatcgt gcggccgtcc gccgtgacaa aacccgcaat
     3421 gtcgatggca ttggcaagcg cgtgctcgct caggcgctcg ctatggctct ggctgccgtt
     3481 gcgctgccgg caggcatagc ccgaggcgtt ggcgaggcgc gtaatcggac tgccgaaagc
     3541 ctgctcggcg gccggctgca gcaccgtcgt cacccattcg tgcaagtggg cgaccatggc
     3601 gcagttgacc atggccggcg gcttgatttc cacgcgcgtg ttgcccgcgg caacgcggcg
     3661 caacatcacg ggagccgggg tgccacattg atcctgcttg accggggcca ccacctcgac
     3721 ttcggccacg atcggcgcca actctttgac gcagtcgcgc agcgcgccga tcacctccgc
     3781 ctcggaccag acgtcgggct tgggtggctc gccttgcgcc gattgagccg gcgcctccgg
     3841 ggccgccgcg ggcgccggcg cggaggcggt gcccggtgct ggcgtcggct cggcttcaac
     3901 cgcgatcgag gagttccgac gcacgggtgc cggcgggtcg ggctcggcgg cgcgccggcg
     3961 ctggcgccgg ctggcgatca gctcgacggc cgcggccggc tcgtcgatga tcacctgctt
     4021 ggggcgcacc atctcgatgg gcggcggact ttgcgccaag cgggccggac gcagggttga
     4081 ggccgcctgt tgtggcagaa tggcaatgct gccgatgccc agcaccgtca cgaccgaaag
     4141 cttggtcgac aactgggcaa gcgcgttggc ggctgcccgc accgccgcgg ccgagcgcgc
     4201 ttgggtccac gccgccagat cagcatacgt ctcggcatac tttgcgcgat agtgcattgg
     4261 gttttggaaa cgcctgacgc cgcggtacgg tttcgcacgc ccttgcccag tcgccgcgag
     4321 catgcgcgcc gcatatgttg gaagtaagga agcggcaggc gctaagcccc gttgctgccg
     4381 agaaattcct ggcgcaccaa ttccgtgtgc gcccccaggg gcgcaatggc gccgaaggca
     4441 gctggagccg cgccggtgcg cgccgggggg gccggcagca ggacggggcc tgagggtgtg
     4501 tcgacgctga tgcggcgcag atgcgggtgt ctgatgatgt cgccgacctc gttgacgcgg
     4561 gcgaaggcga tgtcggccgc ctccagcttc ttgctcaaag tgaggacatc caagctcgca
     4621 aagtgagccg ccaccgcgcc atcggtttgg ctgcgatggg cgagccgcgc cgggctggtg
     4681 gcaaagcgcg gatcggtgcc gagctcgggg cgccccagca ccttgctgca taggctcacc
     4741 cactcgcgat cgttctggat cgagatcaga atgggcaccg cgtccgccgt cgtgaagacg
     4801 ccgtagggcg caagcgaggg atgggttagg cccgaacgac gcggcgcctc ggcatattcg
     4861 gcaaacagca ggggaaccgt catccattcc atcatggcgt cgaacatggc gacgcgaatg
     4921 tcggcaccct cgccggtcct ggcgcgttga atgagggcct cgagaatggc ctcgtaggca
     4981 tgcaaccccg tgccgatgtc gacgagcgag acgccgacgc gcgccggcgc ctccgggccg
     5041 cccgtcaggg aggcgagccc ggattcggcc tgcaccaaga ggtcgtaggc cttgcgttcg
     5101 cgatagggcc cctcctcgcc atagccgctg atggagcaga cgatgaggcg cgggtagcgt
     5161 tggcgcagag cctcgacggc gtagccgagc ttcgccaagg cgccgggctt caggttctgc
     5221 aggaagacgt cggccttggc cagcagcgcc tcgaataggc ggcggtcctc gcctcggcgc
     5281 aggtcgacca ccaccgattc cttgccgcgg ttcagccaga cgaaatgcgt gcattccccg
     5341 tgcacggcct tgtcgtattc gcgcgcaaag tcaccctcgg gacgctcgag cttgatgacg
     5401 cgagcacccg catcggcgag ccgccgcgcc gccattggcg cggccactgc ctgatcgagg
     5461 gaaacgacga gagtgccggc cagcgctcgc atgatgcctt ggcccccgct tagagaaggc
     5521 cgcgccgcgc cagattacgc atcaaggcgg cggtgccgaa gctccacggc ggcgcctcgg
     5581 tcgacagcgt catgcgattg gcgaggctgc cgagctcggg ggcggcgatg gtggtgatgt
     5641 cgcccagctt gtgcgtgaaa ccctgcccgg cggcatcacg atcctcgatc ggcgcaaaca
     5701 tggtgccgag aaacagcacc gctccgtccg gatattggtg atgccgcccc agcatttggg
     5761 ccacgagtgc ggccgggtcg cgcgcgatct cgcgcatgga ggagcgcccg ttcagcacga
     5821 agccatcctc gccctcgacc ttgaggctga cctcgatgcg gcgcacatcc tcgagcgaga
     5881 aactggcgtc gaaaaagcgc acgaacgcgc cgagcgcggt cgatgcattg ttgtccttgg
     5941 ccttggacag cagg
//
LOCUS       DASTMT010000146        11913 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_5291727, whole genome shotgun sequence.
ACCESSION   DASTMT010000146 DASTMT010000000
VERSION     DASTMT010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 11913)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11913)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11913
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5291727"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(781..1392)
                     /locus_tag="VFR00_03135"
     CDS             complement(781..1392)
                     /locus_tag="VFR00_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HEU0158277.1"
                     /translation="MRTPRPRKDLQSSSYLDGQLLVAMPLMTDKRFARSVIYMCAHSD
                     KGAMGLIINQKAPHISFRELLGQLNISTRDPEKLDRELIDISVHVGGPVETGRGFVLH
                     SSDYYAADSTLAIDDSVSLTATVDILKAIAAGRGPGRAILALGYAGWRAGQLESEISA
                     NGWLHCPADPELLFECALDLKYERALSKIGIDPSHLVSVAGHA"
     gene            1647..2543
                     /locus_tag="VFR00_03140"
     CDS             1647..2543
                     /locus_tag="VFR00_03140"
                     /inference="COORDINATES: protein motif:HMM:NF022853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD
                     domain-containing protein"
                     /protein_id="HEU0158278.1"
                     /translation="MGAVAQQGFGIGEVVMCKNWRTAGVLGFLFLAAVLSAAAAEDAA
                     VTPWVEAQAARVRLLAGADQPGKGVLAGVEIVMAEGWKTYWRNPGEAGVPPNFDWSGS
                     SNTASIKVDYPAPMRISDPAAEMIGYKTSVLFPISVMPQVPGEPVDLRLALEFAVCRE
                     ICIPAQAALELKLPPARRLGAPAPALAAAQARVPRAQGSLRASDPRLLAVRSTLSGPS
                     PRLEIDAAFPGGNGGADLFLEPPDGIFVPMAQKMSAAGDGRVRFLVDLARAGNAEELK
                     GKDLKLTLVSDAGASEATVRIE"
     gene            2635..3120
                     /locus_tag="VFR00_03145"
     CDS             2635..3120
                     /locus_tag="VFR00_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013949771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEU0158279.1"
                     /translation="MTIKVGDRLPDTTFMTMSPEGPKPTKTADIFAGKKVALFAVPGA
                     YTPTCHKHHMAGFVANYEELKRKGFATVACTSTNDIFVLSHWAKDSNADGKILMLADG
                     SAEFAKKIGLDIDLTARGLGIRSKRYSMIVEDGVVKSLNVEDAPPNHEKSSAATLCSL
                     A"
     gene            3303..4295
                     /locus_tag="VFR00_03150"
     CDS             3303..4295
                     /locus_tag="VFR00_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEU0158280.1"
                     /translation="MQLWRKTQEVADTTHRVGTEEELLDAYSSAVVRVVEEVSPSVVQ
                     VRVRALRQGHAAQGAGSGSILSPDGLVLTNNHVVEGASAIELATIDARRFPARVLGRD
                     PDTDIAVLRAETSDRLPAARLGNSKLVKAGQIAIAIGNPLGFESTVTAGIVSAVGRSL
                     RAQNGRLIGDVIQTDAALNPGNSGGPLVNSRAQVIGVNTAVIMGAQGICFSVASNTAQ
                     HVVTQILQHGRVRRARLGIAGDQVSLPQRLRAQAGLGQEAGVRVVEVQPGSPAEVAGL
                     EPGDVVIALDQETVTGIDDIARVLDGSKIGKRVSVRVLRDAAQIKTLELVPTER"
     gene            complement(4302..5420)
                     /gene="tsaD"
                     /locus_tag="VFR00_03155"
     CDS             complement(4302..5420)
                     /gene="tsaD"
                     /locus_tag="VFR00_03155"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEU0158281.1"
                     /translation="MPRPSTGPARTAQHGTRVLGIETSCDETAAAVVARTPDGSGEIL
                     ANVVRSQWQEHRSYGGVVPELAARAHVECLDEIIAEAIAGARIGFAELDAVAVTAGPG
                     LIGGLLIGAMTAKAIAVVHRLGLLAVNHLEAHALTVGLTEGLSPPYLLLLVSGGHTQL
                     LIVHAVGRYQRLGSTIDDALGEAFDKTAKLLGLGVPGGAAVEQAAAMGRPGRFRLPRP
                     MLGRPELNFSFAGLKTAVRQQARALVPLAEDDVADLCAEFQAAVADSVVDRVERAMAL
                     AAPVLEGARHLVVAGGVAANQRLRQALAGAAERGGYRLHVPPAALCTDNAAMVAWAGA
                     ERLARGLTDELAFEVRARWPLDPLAPPALGAGKQGAKA"
     gene            5498..6430
                     /gene="hemC"
                     /locus_tag="VFR00_03160"
     CDS             5498..6430
                     /gene="hemC"
                     /locus_tag="VFR00_03160"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012110243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HEU0158282.1"
                     /translation="MQAKTIRIGTRGSPLALAQAREVEARLAAAHPPGAYAYDIQIIR
                     TSGDRIQDRPLGEVGGSKGFFTKEIEEALLAGEIDIAVHSMKDMPTVLPAGLVVTCHL
                     PRQDVRDAFIGRGVASLAELPAGAVVATSSLRRQAQVRRLRPDLRVEAMRGNVETRLK
                     KLADGVAQATILAMAGLNRLGLQDRVSAPMPVDLMLPAVAQGAVGIESRADDFATMRL
                     IAPINHQPTGLTVAAERAFLAKLDGSCRTPIAGLAELDGSGFNFRGMILTPDGREWHE
                     TARRGRPEEAIRLAEEAAAELLARAGPNFLSAIG"
     gene            6430..7164
                     /locus_tag="VFR00_03165"
     CDS             6430..7164
                     /locus_tag="VFR00_03165"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HEU0158283.1"
                     /translation="MRLLITRPEPDALKLQAVLEDMGQEATVEPLLSVSFEDVSRLEL
                     DGVQALIATSRNGVRALRSTPLLPRARALPLFAVGAATAVEARTLGFEMVVTGAGTAR
                     ELVTHIVSVLDPAAGLIVHLAGDTLAVNLKAELEPHGFRVLEWVVYRMQAATGFSDDV
                     VEQLAMGEIDGVILLSPRTAAVYAKLVRAHRLQEIVRPLRHFCLSPAVARRLEPLGQV
                     AQITAQAPKLEEVLALIEETAAKSGD"
     gene            7278..8636
                     /locus_tag="VFR00_03170"
     CDS             7278..8636
                     /locus_tag="VFR00_03170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0158284.1"
                     /translation="MADSKDESGKGASRPYARLDLKATEVDGKPGGSGKATRFSAPNP
                     FAAAQAFLRDQRRTVLLLTHLAAGLLGGALAAGAFLAFGGPREGAGAQAREVAPLTGR
                     VAELERTVARQATAPDLRGRVDGLARATGALEAASSKHAGDLKSLQERLANPAAGTAE
                     LAERLAKLESTIAALSVSGSGEANEAARAGLERFEREVASAKAEAGRLGNRFDGLEQQ
                     LRTSQGAGERLKGELERGLKGTASSEDLSSLKERLAALDKEFQELLKTEANRTANTSR
                     VVLSLELSNLKRAIERGDSFTSELSAAKKVAGEQLNLARFDRYAKDGLPLLSELTKSF
                     RKVANGMLDAEAEPTDAPLLERLLSGAKSIVRVRKSGQTADDGSLEATIARMETALKE
                     NRLGDVLAQGRKLPPKAALAAEDWLRRVEMRYQVEQALAETEAALKRSLAAAPAGTDR
                     RQ"
     gene            8633..10348
                     /locus_tag="VFR00_03175"
     CDS             8633..10348
                     /locus_tag="VFR00_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme biosynthesis HemY N-terminal
                     domain-containing protein"
                     /protein_id="HEU0158285.1"
                     /translation="MIRILIFLLAVSVLAAGLHWLADRPGTIVVEWQDYVAETSVFLA
                     VVLLVLLVALLLLLGWLLTRVWSSPAAVGRLLRRRRLQRGLDAISGGIIAIGAGDGSL
                     ATRYALQARKSLPNEPLTHLLRAQAAELNGDRSTARRIFEAMLASPDTAQLGLRGLFL
                     EAKREDETEAARQFAERALKLNPQLAWPVEALFDLMCKAQDWQGALATLALAQRQNLI
                     DKAVAQRRRAVLLAAKAQEAEDGAPERALELALEAHKLAPDLVPAAAIAGRVLASQGN
                     TPRAARVLLRTWRLSPHPELATVYAYARPGDSPRDRLTRVRHLGRLTPQANEAAIAIA
                     IASIEAHEWDEARKALEPLLGARLTQRVSTLMARIEGEQHGNAGAVREWLARALNAPR
                     DPAWTADGVVSDTWAPTSPVTGALDAFRWRVPVEPIQADGGALLAAKVEALVGLGAGA
                     ASQLRQSAPAVPTPVEVTAIDEAAKRAPALLGKTDPAGTAEAAAPPSMEAPAPEPPAA
                     GPSSKNALGASAGARKAGTNSARSRPAEAKIFVPPRPPDDPGTGAAEDEALLMPDLRG
                     SAAKA"
     gene            10431..11840
                     /locus_tag="VFR00_03180"
     CDS             10431..11840
                     /locus_tag="VFR00_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012321838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HEU0158286.1"
                     /translation="MESFDALTLARLQFAFTISFHIIFPAFTIGLASYLAVLEGLWLI
                     TGRAVFLAVFNYWKKIFAISFGMGVVSGIVMSYQFGTNWSVFADKTGPILGPLLGYEV
                     MSAFFLEAGFLGVMLFGMDRVGKGLHCFATVMVAGGTLLSAFWILAANSWMQTPMGYA
                     SNAAGQFVPADWWAVIFNPSFPYRLVHMVLAAYLTTAFVVGGVGAWHLLADRRNESAR
                     LMFSMAMWMAALVAPLQVVAGDLHGLNTLQHQPAKIAAMEGHWETRAGQPLILFGWPS
                     MAAETTRYAVELPKLGSLILTHDLNGTVKGLKAWPPQERPNATIVFWSFRIMVGVGVL
                     MLLAGAWSLLQRYRRQLTGSSALLRFAVLMSPAGFIAVLAGWITTEVGRQPYTVYGLL
                     RTAQSHSPIDAAAVGASLLAFIAVYLAAFGAGAFYMLRLMLQAPQLDEPDIEKGLPQR
                     AAGITPASALGDVAAAGQK"
BASE COUNT         1907 a         4024 c         3988 g         1994 t
ORIGIN      
        1 cccgcaccgt cggccgcgcc cgccaatggc agcgcagcgc gcgccccaga ggccgccgct
       61 cccctgcccg cgcccgtgcc gcgcccgagg ctcgaggcgc gcgcgaacga gcgccgccct
      121 cgcttgccac cgccgctgcc gcccgcgccc ccggggtcaa gaccgccccc gcccggggcg
      181 ccgggctcgg gtccaccccc tcccgggcct gccgggccaa gaccacctcc gcccgggccg
      241 ccgggatcac gatcaccccc gccggcgcgc cccgcgcagg ccggagcgga ccgcgctggt
      301 aaacctctcc cggccacgcc gccggtcccc actgccaaaa gcgcgcccgg ggcgccgccg
      361 ccggcgcgcg gcaacccgcc gccgcccggc ccgggcggcc cggcgccccg gcccaaaacc
      421 gccccgccgc ccgccaacgg tgcgctgccg cccctggcgg cccgctcgcc cgcgccgcct
      481 ccacccctgc cgcgcaccgc gccgccggtg ccgccggcgc cggcgcggcc caatggtcga
      541 cccggcgccg atttctcgac gctgccgccc gcaattgccg aaagcctggc ccggcttgcc
      601 ggccgacaag gcaccgcgcc ggtggcgcgc gataaggccg agacggacgg ccccggcccg
      661 gccaacaagc ccccgcaacc ttgatcttgc gcgcccttgg tttttgccag cgccgctgcg
      721 gggcctggga ggacggcagc ccgcccgaac ttgtgccgcg ccgccgcctg ctcgggctcc
      781 tcaggcgtgc ccggcgacac tgacgaggtg ggagggatcg atgccgatct tggagagcgc
      841 tcgctcatat ttcagatcga gcgcacactc aaacaacaac tccggatcgg cgggacaatg
      901 cagccaaccg ttcgcgctaa tctcgctttc gagttgcccc gcgcgccaac ccgcatagcc
      961 aagcgcgagg atcgcccggc caggccccct gccggcggcg atggctttga gaatgtccac
     1021 ggtcgcagtc agcgagacgc tgtcgtcgat ggccagggtt gaatcggctg cataatagtc
     1081 ggaactatgc aagacgaaac cacgcccggt ctccaccggt ccgccgacgt gcacgctgat
     1141 gtcgatcaat tcgcggtcga gtttttcggg atcgcgcgtg gagatgttga gctgtcccaa
     1201 caattcccga aagctgatgt gcggcgcctt ctggttgatg atcaatccca tggcgccctt
     1261 gtcggaatgc gcgcacatgt agatcacgga gcgcgcaaat cgcttgtccg tcatcagcgg
     1321 catggccacc agcaattgcc cgtccagata gctgctggac tgcaagtcct tgcgaggtct
     1381 cggcgtgcgc atgctggaag actactgcct cacaggtgcg cctgtgaagg ggctcaaggg
     1441 gtcacgctga aatcgaggag acttgagttt ggcggcgcgc cgccgcatgg cataggattt
     1501 gcctgatagc ccaggatttg cctaatggcc tagggtttac ccggtagctt ccgccgaagt
     1561 ctcctcggcc gaaagtcctg gaccaaaggt cctccggcga ccccttgggc ccaagcctcc
     1621 ctgcgctaaa tgtggagggc ccgattgtgg gcgccgtcgc gcagcaggga tttggcattg
     1681 gcgaggtggt gatgtgtaaa aactggcgca cggccggcgt gctgggattt cttttcctgg
     1741 ctgcggtcct ttccgctgcg gccgccgaag atgctgccgt cacgccctgg gtcgaggcac
     1801 aagccgcgcg cgtgcgcttg ctggcgggtg ccgaccagcc gggaaagggc gtgctcgcgg
     1861 gggttgagat cgtcatggcc gaggggtgga aaacctattg gcgtaatccc ggggaggcgg
     1921 gagttcctcc gaactttgat tggtcgggct cgagcaatac ggcctcgatc aaggtggact
     1981 atccggctcc catgcggatc agcgatcccg ccgccgaaat gatcggttac aagacatcgg
     2041 tcctctttcc gatcagcgtc atgccgcagg tgccgggcga gccggtcgat ctacgcctcg
     2101 ccttggagtt tgcggtctgc cgcgagatct gcattcccgc ccaggccgcc cttgaattga
     2161 aactgccgcc agcacgccgc ctcggtgcac ccgccccggc gttggctgcc gcccaggcgc
     2221 gcgtgccgcg cgcgcagggc tctttgcgtg ccagcgaccc tcggctgctc gcggttcgca
     2281 gcacgctcag cggtccttcc ccgcggcttg agatcgatgc cgcctttccc ggcggcaacg
     2341 gcggtgccga tctgtttctc gagcctcctg atgggatctt cgtgcccatg gcgcagaaaa
     2401 tgagcgccgc tggcgacggg cgtgtgcgct tcctcgtcga cctggcgcgg gcgggaaacg
     2461 ccgaggagct caagggcaaa gacctcaagc tgaccctcgt cagtgacgcc ggcgccagcg
     2521 aggccaccgt gcggatcgaa taggggcggc ttggcttgcc tggcacgagc gcctaagttg
     2581 acggcgcgct tagaacccat gccgcagagc tcatagagca ggaaaggacg agccatgaca
     2641 atcaaggtcg gcgaccggct gcccgacacg accttcatga cgatgtcacc cgaaggtccc
     2701 aagcccacca aaaccgccga tattttcgcc ggcaagaagg tggcgctgtt cgccgtgccc
     2761 ggtgcctaca cgcccacatg tcacaagcat cacatggcgg gtttcgtcgc caattacgag
     2821 gaactgaagc gcaagggctt cgcgacggtc gcctgtacgt cgaccaatga cattttcgtg
     2881 ctgagccact gggccaagga ttccaacgcc gacggcaaaa tcctgatgct ggccgatggt
     2941 agcgccgagt tcgccaaaaa gatcgggctc gacatcgatc tcacggcgcg cggtctgggc
     3001 attcgctcca agcgctattc gatgatcgtg gaggacggcg tcgtcaaatc cctcaatgtc
     3061 gaggatgccc cgcccaacca cgaaaagtcc agcgccgcca cgttgtgcag cctcgcctga
     3121 cgcggcgttt tttcgctgct cttacggcag cttggggaaa gatctgaccc cgagttgcgg
     3181 ggcgctctcc ggagccagag ggccgccgca tcggcggcgc ccctccccgg acgcaattcc
     3241 gttcctatat tactgccccg atcgctgtgc gcgggaactc ttatttcgtc ggaggtggcc
     3301 acatgcaatt gtggcgcaaa actcaagagg tggccgatac gacccatcgc gttggcaccg
     3361 aggaggagtt gctcgatgcc tattcgagcg ccgtcgtccg cgtggtcgaa gaggtgtccc
     3421 catcggtcgt gcaagtgcgc gtccgcgctc tgcggcaggg acatgccgcc cagggcgccg
     3481 gctcgggcag tattctttca cccgacggtc tggtgctgac caacaaccat gtggtcgagg
     3541 gcgccagcgc gattgagctt gccaccatcg atgcgcgccg ctttcccgcg cgcgtgttgg
     3601 ggcgcgatcc ggacaccgac atcgcggtgt tgcgggcgga gaccagcgat cggttgccag
     3661 cagcgcgtct cggcaactcc aagctcgtaa aagccgggca aattgccatt gccatcggta
     3721 atccgctggg cttcgaatcc acggtcacgg cgggtatcgt cagcgccgtt ggccggtcct
     3781 tgcgcgcgca gaacgggcgc ttgatcggcg acgtgatcca aacggacgcc gcgctcaatc
     3841 ccggcaattc cggcggaccg ctggtcaatt cccgcgccca ggtgattggc gtcaacacgg
     3901 ccgtaatcat gggtgcgcaa gggatttgtt tttccgtcgc ctccaacacc gctcagcacg
     3961 tcgttacgca aattctgcag catggccgag tgcggcgcgc acgcctgggc attgccggtg
     4021 atcaagtcag tctgccccag cgcctgcgcg cccaggcggg gctcggccag gaggccggcg
     4081 tgcgcgtggt ggaggtgcag cctggcagtc cggccgaggt ggccgggctt gaacccggcg
     4141 acgtggtgat tgccctcgat caggagaccg tcaccggcat cgacgacatt gcccgcgtgc
     4201 tggatggcag caagatcggc aagcgcgtca gcgtccgtgt cttgcgcgat gccgctcaga
     4261 tcaagacgct cgagctcgtg cccacggagc gctgaagcgc ctcaggcctt ggcaccctgc
     4321 ttgccggcgc ccagggcagg cggggccaag ggatcgagcg gccagcgcgc gcgaacttcg
     4381 aatgcaagct cgtccgtcag cccccgtgcc aaccgctcgg caccggccca ggccaccatg
     4441 gcggcattgt cggtgcacag cgccgccggc ggcacgtgca ggcgataacc gccccgctcg
     4501 gccgcgccag cgagcgcctg gcgcaggcgc tgattggcgg caaccccacc cgccaccacg
     4561 aggtgacggg cgccctcgag cactggggcc gccagggcca tggcccgctc aacccggtcc
     4621 acgacgctgt cggcaaccgc cgcctgaaac tcggcgcaca ggtcggcgac atcgtcttcg
     4681 gccaagggga cgagggcgcg ggcctgctgg cggaccgccg ttttcaggcc ggcgaaggag
     4741 aagttgagct cgggccggcc tagcatcggc cgcggcaacc tgaagcggcc ggggcgaccc
     4801 atcgccgccg cctgctcgac ggcggcgccg ccggggacac ccaggcccaa gagcttggcc
     4861 gttttgtcga aggcctcgcc cagggcgtca tcaatggtgc tgccgagccg ttggtagcga
     4921 ccaaccgcgt gcacgatcag gagctgcgtg tgtccgcccg acaccaatag caacagatag
     4981 gggggcgaca aaccttcggt caaaccgacg gtcagggcat gcgcttccag atggttgacg
     5041 gcgaggaggc ctaaacggtg aaccacggcg atggctttgg ccgtcatggc cccgatcagg
     5101 agcccgccga tcagtcccgg tccggccgtg accgccaccg cgtcgagctc ggcaaagccg
     5161 atccgcgcac cggcaatggc ctcggcgatg atctcatcga ggcattcgac atgggcgcgg
     5221 gcggcgagtt ccggcacgac gccgccgtag ctgcgatgtt cttgccactg ggaacgcacg
     5281 acattggcca gaatttcccc gcttccgtcg ggggtgcgcg ccacgaccgc cgcggcggtt
     5341 tcgtcgcagc ttgtctcgat gccgagcacg cgcgtgccat gctgcgctgt cctcgcaggt
     5401 cctgtggatg gtctaggcat tatggcgttg gcgcggcgtc cttgctatga ggctaggttg
     5461 taatgatgtc gaccgcctag caccggaaca atcgaccgtg caagccaaga ccattcgtat
     5521 cggcacgcgg ggctccccgc tggcgctggc gcaggcccgt gaagtggagg ccaggctcgc
     5581 cgccgcacac ccgccgggtg cctatgccta cgatatccag atcattcgca cctccggtga
     5641 ccgcatccag gaccgcccgc ttggtgaagt tggcggcagc aaaggcttct tcaccaaaga
     5701 gatcgaggag gcgctgctgg cgggcgagat cgacattgcc gtccactcca tgaaagacat
     5761 gccaaccgtg ctcccggccg gattggtcgt cacctgtcat ttgccgcgcc aagacgtgcg
     5821 cgatgccttc atcggtcgtg gggttgccag cctggccgag ctgccggcgg gtgctgtcgt
     5881 tgccacctcc tcgctgcggc gtcaggcgca agtgcggcgg ttgcgccccg atcttcgcgt
     5941 tgaggcgatg cgcggcaatg tcgagacccg gctcaaaaag cttgccgacg gcgtggccca
     6001 ggccaccatt ctggccatgg ctggcctcaa ccggctgggc ttgcaggatc gggtgagcgc
     6061 gccaatgccg gtcgacctga tgctgccggc cgtcgcccaa ggcgccgtcg ggatcgaaag
     6121 cagggccgac gatttcgcca ccatgcgttt gatcgctccc atcaatcacc agccaaccgg
     6181 cttgacggtt gccgccgagc gggccttcct cgccaagctc gatgggtcct gccgcacccc
     6241 gattgccggg ctcgccgagc tcgacggcag cggctttaat ttccgcggca tgatcctgac
     6301 gccggatggc cgcgagtggc atgagacggc ccgccgggga cggcccgagg aggcgatccg
     6361 tctcgccgag gaggccgccg cggagctgct cgccagggct gggccaaact tcctcagcgc
     6421 catcggctga tgcgcctgct catcacacgt ccagaacccg acgcccttaa gttgcaggcg
     6481 gtgctcgagg acatggggca ggaggccacc gtcgaaccgc tgctcagcgt cagcttcgag
     6541 gacgtgagca ggctcgagct cgatggcgtg caggcactca tcgccaccag ccgcaatggc
     6601 gtacgcgcct tgcgttcgac accgctcctg ccccgggcgc gcgcgctgcc gctgttcgcg
     6661 gtgggcgcag ccacggcggt tgaggcgcga acgctcggct tcgagatggt cgtcaccggg
     6721 gccggtaccg cccgcgagct cgtcacccac atcgtctccg tgctcgaccc cgcggcgggg
     6781 cttatcgtcc atctcgcggg agatacgctc gccgttaacc tcaaggcgga gctcgagccg
     6841 catggctttc gcgtgctcga gtgggtggtc tatcgcatgc aggcggcgac cggtttcagc
     6901 gacgacgtgg tggagcaact ggccatgggc gaaatcgacg gcgtcatctt gctctcacca
     6961 cgcaccgcgg ccgtctacgc caagctggtg cgcgcccatc ggctgcaaga gatcgtccga
     7021 ccgcttcgac acttctgtct ctcccccgcc gtcgcccgcc ggctggagcc gctggggcag
     7081 gtggcgcaga tcacggcgca agcgccgaag ctggaagaag tgcttgctct catcgaggag
     7141 accgcggcaa aatcggggga ctagccgggg cctactcgcg cgcgtaaaag tggcgccgcg
     7201 ttgccgaccg tgcccgccga gcattgctgc ctttcgcacg ttgcacgcgg tgtgtcccgg
     7261 agctcaggaa gccactgatg gccgatagca aggacgaaag cggcaaaggc gccagccggc
     7321 cttacgccag gctcgatctc aaggccaccg aggtggatgg caagccgggt ggcagcggca
     7381 aggcaacacg cttctcagcg cctaacccat tcgccgcagc gcaagccttt ctgcgcgatc
     7441 agcgacgcac ggtgctgctt ttgacgcacc tcgcggcggg tctgcttggt ggcgcgctcg
     7501 ccgcgggtgc gttcctcgct ttcggcggtc cccgggaagg cgcaggcgcg caggcgcgcg
     7561 aggtggcacc gcttacgggg cgcgtggccg aactcgagcg cacggtggcg cgccaggcga
     7621 cggcaccgga tttgcgcggc cgcgtggatg gattggcgcg cgccactggc gcccttgagg
     7681 ccgccagcag caagcacgcc ggcgatttga agagcttgca ggagcgcctc gctaaccccg
     7741 cggccggaac cgctgagctc gctgagcggt tggcaaagct cgagagcacc attgcggcgc
     7801 tcagcgtctc gggcagcggc gaggccaacg aggcggcgcg ggcgggcctc gaacgcttcg
     7861 agcgcgaggt tgcgagcgcc aaggccgagg ccggccgact cggcaatcgc ttcgacggtc
     7921 ttgagcagca gctgcgcacc tcgcaaggcg ctggcgaacg gctgaagggc gagctggagc
     7981 gcggcctgaa gggcacggcc agcagcgagg atctgagttc gctcaaagag cgcctcgccg
     8041 ccctcgacaa ggagtttcag gagcttttga agacggaggc caatcgcacg gccaacacgt
     8101 cgcgcgtcgt cctgtccctg gagttgtcca atctcaagcg ggcgatcgaa cggggggata
     8161 gcttcaccag cgagctcagc gcggccaaaa aagttgccgg cgagcagttg aacctggcgc
     8221 ggttcgaccg ttatgccaag gacggtttgc cgctgctttc ggagttgaca aagtctttcc
     8281 gcaaagtcgc caatggcatg cttgatgccg aagccgagcc gaccgacgct ccgctcctgg
     8341 agcgactctt gtcgggtgca aaatccatcg tgcgcgtgcg caagagcggg cagacggccg
     8401 acgacggcag cctcgaagcg accatcgccc gcatggagac ggcgctgaag gagaaccggt
     8461 tgggcgacgt actcgcacag ggcaggaagc tgccgccgaa agcggccttg gccgccgagg
     8521 attggttgcg gcgcgtcgag atgcgctatc aggttgagca ggcgctggcc gaaaccgaag
     8581 cggccttgaa gcgctcgctg gcggcagccc cggccgggac ggaccggcga caatgatccg
     8641 tattctgatt tttctccttg ccgtcagcgt tcttgctgcc ggcctacact ggctggctga
     8701 tcgccccggc acgattgtgg tggagtggca ggactacgtg gccgagacca gcgttttcct
     8761 cgccgtcgtg ctgctggttt tgctcgtcgc cttgctgttg ttgctgggat ggctgctgac
     8821 gcgggtctgg tcgagccccg cagcggtcgg gcgccttctg cggcggcggc gcctgcagcg
     8881 cgggctcgac gccatctcgg ggggcatcat cgccatcggc gcgggcgacg gcagtttggc
     8941 gacgcgctat gctctgcagg cgcgcaagtc cttacccaac gagccactga cgcacctctt
     9001 acgcgcccag gcggccgagc tcaatggcga tcgaagcacg gcgcgccgca tcttcgaggc
     9061 catgctggct tcgcccgata ccgcccagct cggattgcgc ggtcttttcc tggaggctaa
     9121 gcgggaggat gaaacggagg cggcgcggca atttgccgag cgtgccctaa agctcaatcc
     9181 tcaactggcc tggccggtcg aggccctgtt cgatctcatg tgcaaggcgc aagactggca
     9241 aggggcgctc gccaccctgg cgctagcgca acgtcagaac ctgatcgaca aggccgtggc
     9301 gcagcggcga cgcgccgtat tactggccgc caaggcgcag gaggcggagg acggcgcgcc
     9361 cgagcgcgcg ctggagctgg ccttggaggc gcacaagctg gcacccgacc tggtacctgc
     9421 ggcggccatc gcgggacgcg tgttggcctc gcagggaaac acgccgcgcg ccgcgcgcgt
     9481 gctgctgcgc acctggcgct tgtccccgca tccggagctg gcgaccgtgt acgcgtacgc
     9541 ccggcccggc gatagcccgc gtgatcggct gacgcgcgtg cgtcatcttg gacgcctgac
     9601 gccgcaagcc aacgaagccg ccattgccat tgccattgcc tcgatcgagg cacacgagtg
     9661 ggacgaggcg cgcaaggcgt tggagcccct gttgggagcg cgcctcacac aacgcgtctc
     9721 gacgctgatg gcgcgcatcg agggcgagca gcacggtaac gccggtgcgg tccgcgagtg
     9781 gctggcgcgg gccctcaatg cgccccgcga tccggcctgg accgccgacg gggtcgtttc
     9841 ggacacatgg gcgccgacct cgcccgttac cggcgcgctc gatgccttcc gttggcgggt
     9901 gcctgttgag ccgatccagg ccgacggcgg cgccttgctt gccgcgaaag tcgaggccct
     9961 cgtgggtttg ggcgccgggg ctgcctcgca actgcggcag agtgcgccag ccgtgccgac
    10021 gcccgtcgag gtcacggcga tcgacgaggc cgccaagcga gctccagcgc tgctgggcaa
    10081 aaccgacccc gccggcactg cggaagccgc cgcaccgcca agtatggagg ccccggcgcc
    10141 cgagccgccc gcggcaggcc cgagcagcaa gaacgcgctc ggtgcgagcg caggcgcgcg
    10201 caaagccggc accaattccg cacgttcgcg gccggccgag gccaagatct ttgtcccgcc
    10261 aaggccgccg gacgatcccg gcacgggtgc tgcggaggac gaagcgttgc tgatgcccga
    10321 cttgcgtggt tccgccgcca aggcatgatc gctgctttcg cagtcctgcg gaaaggcgca
    10381 agctcggttg ccctgcgagc gctacaaagc ttgccaagga ggcgttggaa atggagtcct
    10441 tcgacgcgct gaccttggcc cggctgcagt tcgccttcac catttcattt cacatcatct
    10501 ttcccgcctt cactattggg ctcgccagct atctcgcggt gctcgaaggg ctttggctga
    10561 tcacggggcg tgccgtcttt ttggcggtct tcaattactg gaagaagatt tttgccatca
    10621 gcttcggcat gggggtcgtg tcgggcattg tgatgagcta ccagttcggc accaactgga
    10681 gcgtctttgc cgacaagacc ggacccatcc tcgggccatt gttgggctac gaagtgatgt
    10741 cggccttctt tctcgaggcc ggcttcctgg gcgtgatgct gttcggcatg gatcgggtgg
    10801 gcaagggtct gcattgcttc gccaccgtga tggtggcggg tggaaccttg ctttccgcgt
    10861 tctggattct tgccgccaac agctggatgc aaacgccgat gggatacgcc agcaatgcgg
    10921 cgggacagtt cgtgcccgcc gactggtggg cggtcatttt caatccgtcg tttccctatc
    10981 ggctggtcca catggtgctc gccgcttacc tgaccaccgc ctttgtggtg ggcggcgtgg
    11041 gtgcttggca cctcttggcc gaccgccgca acgaaagcgc gcggcttatg ttctcgatgg
    11101 ccatgtggat ggcggcgctg gtggcgccgc tgcaagtcgt ggccggcgat ctgcacggcc
    11161 tcaacacgct tcagcaccag cccgccaaga tcgcggccat ggaaggccac tgggaaaccc
    11221 gtgccggcca accgctaatc cttttcggct ggccgagcat ggcggcggag accacgcgct
    11281 atgccgtgga gctgccaaag ctcggcagct tgatcctgac gcacgatctg aacggcaccg
    11341 ttaagggtct gaaggcctgg ccgccgcagg agcggccaaa tgcgacgatc gtgttttgga
    11401 gttttcgcat catggtgggc gtcggcgtgc tgatgctgct ggccggagcc tggagcttgc
    11461 tgcagcgcta ccgtcggcaa ctgaccggga gctcggcgct gttgcgcttt gccgtgctga
    11521 tgagcccggc cggcttcatc gccgtcctgg cgggttggat aaccaccgag gtcggtcgac
    11581 aaccctatac cgtctacgga ttgttgcgca ccgcgcaatc ccactcgccg attgacgccg
    11641 ccgccgtcgg ggcctcgctc ttggccttca tcgccgttta cctggcggca ttcggcgccg
    11701 gcgcctttta catgctgcgg ctgatgctgc aggccccgca gctcgacgag cccgatatcg
    11761 aaaaaggcct gccccagcgc gctgccggca ttacgcccgc gtcggcgctg ggcgacgttg
    11821 ccgccgcagg gcaaaagtga gggactgcca tgatcgacct gccgttcatc tgggccgccc
    11881 tgatcgcctt tgccgtactc gcctacgtcc tgt
//
LOCUS       DASTMT010000147         4133 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_2049066, whole genome shotgun sequence.
ACCESSION   DASTMT010000147 DASTMT010000000
VERSION     DASTMT010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4133
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2049066"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..531)
                     /locus_tag="VFR00_03185"
     CDS             complement(<1..531)
                     /locus_tag="VFR00_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013499872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU0158287.1"
                     /translation="MATLAGKTLFITGASRGIGLAIALRAARDGANIAIAAKTTEAHP
                     KLEGTIYTAAEAIEQAGGKALPLRCDIRFEAEVEAAVAETARTFGGIDICVNNASAIS
                     LSPAQATDMKRYDLMHGINARGTFVVSKACIGYLKQAANPHILMLSPPLDMSGKWFAP
                     HVAYSMAKFNMSIIALG"
     gene            complement(646..2346)
                     /locus_tag="VFR00_03190"
     CDS             complement(646..2346)
                     /locus_tag="VFR00_03190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0158288.1"
                     /translation="MRGYARAMEIDRASLLGRRAGPGPQARDGRKPLARIAFLAALVL
                     APQVAAADWLGTLIGAARRVAAEAPSAGARALDKVAAHIRSMPSSPNATVLAAQATPE
                     GHWRFVNKAGETITAGTPEEMRRVTSLLVPDARAEARLQLYLTEDTVFARRAAFKDLP
                     RGSELYLFVADESYRILRRSEAANERLFAEIRPNLVVELSDAQQFAEAAWQLRRPLNK
                     ADIRVLALEPGSATRLASAPRLDPASKKALIDSIDPGSLAAALGAVRGQTVLITGRID
                     GELLHFKGANGPERSLLVRDLFKAAEQADVNLIVLTAASTPRQPGGRNWLWQKVEVKG
                     LEEALQRARVADFYNALAPPNGRFAVTAKAAGHLRTQVAVVPIADLPKLPASRPLSDI
                     LGDAAASLTGRVITTGIEANIRSAAHQRDIDWRLFSVLPANVQLGYFALLLVGFLGVP
                     LARVWWAHLWPPEDPAEYAGRTGYWAARLIRDSVFVAVFLPLTAPLSAPINLGRQVWD
                     AVLAPVRAWRRLTGGKRRAAAPSSTEAALGSSQVALGSPLSARHAAEAPSLGSYRPNR
                     "
     gene            complement(2825..>4133)
                     /locus_tag="VFR00_03195"
     CDS             complement(2825..>4133)
                     /locus_tag="VFR00_03195"
                     /inference="COORDINATES: protein motif:HMM:NF025068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M12 family metallo-peptidase"
                     /protein_id="HEU0158289.1"
                     /translation="GQELVLEALKASNEAMVVGVMASLADAPTEYALTRDAQGKITAT
                     RPGVRVIIPFSDKEQVTAVRKSIEKKTQGWTWRGEIEGTGEPVMLMWWKGGKFTGVFS
                     YRGHIYTLRNIGGDVVAILETEPSKLPPDHGPMASQASGQKIALKDDPLFSRGEGAAV
                     RDLQASKDGASESKGWLRTAGTWPAAADLASGQVSSEASDRIVPLAAAKRRQLAAKSI
                     TIDVMILYTPKVAKRYLDIDTDLIALSIEQANQSFAASGIGNIQLRLAHHQQIDYDES
                     KGGHFEHLYRMVDGQGPFESVHALRDEKRADVVVLIVDDASGCGLSTRVAAGADEAFS
                     VVHNTCAVLTYSIAHEIGHIIGARHEKAMDASTTPFPYGHGYVNGTKWRDIMSYRQSC
                     NGCPRVPYWSNPTIKIRGETAGSADADNARVILEQAERVSKFR"
BASE COUNT          672 a         1294 c         1404 g          763 t
ORIGIN      
        1 gcccagcgca atgatgctca tgttgaactt ggccatcgag taggcaacgt gcggcgcgaa
       61 ccacttgccg ctcatgtcga gcggcggcga caacatcaga atatgcgggt tggcggcctg
      121 cttgagatag ccgatgcacg ctttggagac gacgaaggtg ccgcgcgcgt tgatgccgtg
      181 catcaggtcg tagcgcttca tgtcggtggc ctgcgccggg ctcagcgaaa tggcgctggc
      241 gttgttgacg caaatatcga tgccgccgaa ggtgcgagcc gtttcggcaa cggcggcctc
      301 gacctcagct tcgaagcgaa tatcgcagcg cagcggcagc gccttgccgc cggcctgctc
      361 gatggcttcg gcggccgtgt agatggtgcc ttcgagcttc gggtgcgcct ccgtcgtctt
      421 cgcggcaatg gcgatgttgg cgccgtcgcg cgcggctctg agcgcaatgg caaggccgat
      481 accgcggctg gcgccggtga tgaacagggt cttgccggca agcgtagcca tctcaagtcc
      541 tcccgtgtct cacgctgtca tagcgggacc ggccccccgt cgctaggggg atggctgggg
      601 atgcgggttg gtggcccgtg tcgcgccaac gcattgccgc agtcatcagc ggttcggacg
      661 gtagctgccg aggcttgggg cctccgcggc gtggcgcgcc gagagggggc tgccgagcgc
      721 cacctgcgag ctgcctaatg cggcctcggt gctcgatggc gcggccgcgc ggcgtttgcc
      781 gcccgtcagc cgacgccagg cgcggaccgg tgccagtacg gcgtcccaga cctgtcgacc
      841 gaggttgatg ggcgcgctca agggtgcggt cagcggcagg aagaccgcca caaacacgct
      901 gtcgcgaatg agccgtgccg cccagtagcc ggtgcggccg gcgtactcgg cgggatcctc
      961 gggcggccac aggtgggccc accagacgcg ggccagcggt acgccgagga agccgaccag
     1021 cagcagggcg aaatagccga gctggacgtt ggcgggcagg acgctaaaga ggcgccaatc
     1081 gatgtcgcgc tggtgcgccg cgctgcggat attggcctca atacccgtgg tgatgacgcg
     1141 gcccgtgagc gaggcggcgg cgtcacccag aatatcgctg agcgggcgcg aggcggggag
     1201 cttcggcaga tcggcgatcg ggacgaccgc aacctgcgtg cgcaggtgac cggcggcttt
     1261 ggccgtgacg gcgaagcggc cgttgggcgg cgccagggcg ttgtagaagt cggcgacgcg
     1321 cgcccgttgc agcgcctcct cgaggccctt gacctcgact ttctgccata gccagttgcg
     1381 cccgccgggc tggcgcgggg tcgaggccgc ggtgagcacg atcaggttga cgtcggcctg
     1441 ctcggcggcc ttgaacaggt ccctgaccag caggctgcgc tcggggccgt tcgcgccctt
     1501 gaaatgcaag agttccccgt cgatacggcc ggtgatgagc acggtctgtc cgcgcacggc
     1561 gcccagcgcc gccgccaggc tgccgggatc gatgctgtcg atcagcgcct ttttgctggc
     1621 gggatcgagg cgcggggcag aagcaaggcg cgtggcgctg cccggttcca gggcgagcac
     1681 gcggatatcg gccttgttca gcgggcggcg aagctgccag gcggcctcgg caaactgctg
     1741 ggcatcgctc agctcgacca ccaggttggg gcgtatttcc gcaaatagac gttcgttggc
     1801 cgcctcgctc cggcgcagga tgcgatagct ttcatcggcc acgaacagat agagctcgct
     1861 gcctcttggc aggtccttga aggcggcacg acgggcaaac acggtgtctt cggtgagata
     1921 gagctggagc cgcgcctcgg ccctggcgtc gggcaccaag agggaggtga cgcggcgcat
     1981 ttcctctggt gtgccggcgg tgatggtctc gccggccttg ttgacaaagc gccaatggcc
     2041 ctcaggggtg gcctgggcgg caagcacggt tgcgttgggg ctcgagggca ttgatcggat
     2101 gtgggctgcg accttatcga gcgcccgcgc gccggcgctt ggggcctcgg ccgccacccg
     2161 ccgcgccgcg ccgatgaggg tgcccagcca atcggccgcg gcgacctgcg gcgccagcac
     2221 caacgccgcc agaaaggcga tccgcgccaa tggcttgcgg ccgtcccggg cttgcggccc
     2281 ggggccggcg cgccggccaa gcagcgatgc gcgatcgatc tccatggcgc gagcatatcc
     2341 gcgcattttg gcgaacccgg gccgcaatgg cgcgagatcg gaggcgatca aagccgcccc
     2401 gcgtcgcgac gcgaagcttt gccttggcag acggtctcgt tccggcgtgc gccaacgaca
     2461 ggacgtttgc gacgttcgcc agattgatgg cggacgcccg cgagcgtgtt aagcagcaaa
     2521 aacacgtgtg cgcgtccttg catgcgaacc ggttaggcgc actgctgtga agctctgatc
     2581 gagagcccga gttggattga gtatctcggc agcagcgcgc gtgaggttcg gtccctctgc
     2641 gcaaattccg ctcgtcccgt cttggccaga tggaggcctt ttcggaccgc ggcctattgc
     2701 gcgcataccg caatcgcttg ggctgtcagc aagcgacggc ctcgcctttg gcctggtcga
     2761 gacgctcgat ctgccttgcc gctgtcgcca actattctct gaccatcacc gaaggcgcaa
     2821 gcgctcatcg gaactttgag acccgctcgg cctgttccag gatgacccta gcattgtcgg
     2881 catcggccga gccggccgtc tcgccccgga tcttgatggt cggatttgac caatagggca
     2941 cgcgcggaca gccattacaa ctctgcctgt aactcatgat gtctcgccac ttcgtgccat
     3001 tcacgtagcc atgaccgtat ggaaaaggcg tggtgctcgc atccattgcc ttctcgtgac
     3061 gcgcgccgat gatgtgaccg atctcgtgcg caatcgaata ggtaagtacg gcgcaggtgt
     3121 tgtgaacgac gctaaaagcc tcgtcggcgc cggcggcgac acgcgtcgac aatccgcaac
     3181 ccgaagcgtc atctacgatc agcaccacaa catcggcccg cttttcatcg cgcaaggcat
     3241 gcacgctttc gaatggacct tgaccgtcga ccatccggta gaggtgttcg aagtgcccgc
     3301 ccttgctctc gtcgtagtcg atctgctgat gatgggcgag ccgaagctgg atgtttccaa
     3361 tgccgctggc tgcgaacgac tgattggcct gctcgattga gagcgcaatc aggtcggtgt
     3421 caatgtccag atatcttttg gcgaccttgg gcgtgtacag gatcatgaca tcgatggtta
     3481 tgcttttagc ggccagttga cggcgcttcg cagcggcaag cggtacgatc ctgtcgctgg
     3541 cctcgctgct gacctggccg gaggcaaggt cagcggcagc gggccaggtt cccgcggtac
     3601 gtagccaacc cttgctctcg cttgcgccgt ccttggaagc ctgtaggtcc cgcacggctg
     3661 caccctcgcc gcgcgagaac aagggatcgt ctttcagagc tattttctgg cccgatgctt
     3721 gtgacgccat cgggccgtga tcggggggta gcttagacgg ttcggtctca aggatggcaa
     3781 ccacatcgcc gccaatgttt ctgagcgtgt agatatggcc tcggtaacta aagaccccgg
     3841 tgaacttgcc gcctttccac cacatgagca tgaccggctc accggtgccc tcgatttcac
     3901 cacgccacgt ccaaccctga gttttctttt cgatgctttt gcggacggcg gtcacctgct
     3961 ctttgtcact aaaggggatg atcaccctta cgccgggacg cgtcgcagtg attttgcctt
     4021 gcgcgtcgcg cgtcagggcg tactcggtgg gggcatcggc gagggaggcc ataacgccga
     4081 cgaccattgc ctcgttcgat gctttgagcg cctcgagaac cagctcctgg ccg
//
LOCUS       DASTMT010000148         2795 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_2050036, whole genome shotgun sequence.
ACCESSION   DASTMT010000148 DASTMT010000000
VERSION     DASTMT010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2795)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2795)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2795
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2050036"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            256..1098
                     /locus_tag="VFR00_03200"
     CDS             256..1098
                     /locus_tag="VFR00_03200"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: protein motif:HMM:NF000843.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEU0158290.1"
                     /translation="MPQFAPDRFGHIKGLPLTAPGQRIGLLGGSFNPPHLAHRQISEI
                     VLKRLGLDQIWWLVTPGNPLKAPGDLAPLAERLVLCRAMAKNPRIVVTAFERDLPAAY
                     TAGTLAFLKRRSPLVRFVWIMGADNLAGFHRWQRWREILTMVPIVVVDRPGWRLKALA
                     SKAARAFAASQVPETKARGLVAMPPPAWTFLTGPLSPLSSTDLRRKARQRRPSVARKT
                     QSAEPAAAALGASRRPSEAARARTGDATVSEPPQADRAPARVVGRGETRSRRRPLKGT
                     PQAS"
     gene            1245..1655
                     /gene="rsfS"
                     /locus_tag="VFR00_03205"
     CDS             1245..1655
                     /gene="rsfS"
                     /locus_tag="VFR00_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEU0158291.1"
                     /translation="MPTALEGARTSTPPAELVSPEHGSEALLAIITAALDEAKAEAVV
                     AIDIKGKSSIGDYMVIASGRSDRHVGAIAEQLQHKLKDAGYGRARIEGQPQCDWVLID
                     AGDIIVHVFRPEVREFYNLEKMWSAERPSDPTTH"
     gene            1720..2202
                     /gene="rlmH"
                     /locus_tag="VFR00_03210"
     CDS             1720..2202
                     /gene="rlmH"
                     /locus_tag="VFR00_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009734610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEU0158292.1"
                     /translation="MRVVIAAIGRLKDGPERVLFERYRGRFEAAGRRLGFAPVECGEA
                     AESQAASVAKRKTEEAAALRKLTRDAETVIALDAAGKACTSAGFAELLAKIRDEGSRG
                     LAFAIGGPDGLAADILGAAKMRLSFGAITLPHQLARIVLAEQLYRAATILAGHPYHRA
                     "
     gene            2290..>2795
                     /locus_tag="VFR00_03215"
     CDS             2290..>2795
                     /locus_tag="VFR00_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017486115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HEU0158293.1"
                     /translation="MTRNTLAPETVAAQAGDAGDEATGAIVPPVHLATTFVRDPDNQY
                     RKGFSYGRPDNATVRQVERVLAELEGGAGALVVASGMTAATMAFLALERPAHVVAPKV
                     MYWGLRDWISRDAPSFGIEPSFVDGADPAAIAAAIRPGATKLVWLETPANPLWTITDI
                     AAAAKLAHA"
BASE COUNT          466 a          922 c          924 g          483 t
ORIGIN      
        1 ctatggcctg gcaaccgtgg caatcgccgt ggccgctggc ctgctggcat cgacggtttt
       61 gcgccgcggt gcttcctagt ccaggctcaa tacccagctc aatgctcaac gcggcccgct
      121 gccgcattta ttggcaagcg gtcccaagcg ctgcccgcgc aagcggcagc gaagggcctt
      181 tcgggcgcca atgcccctaa gctagactgc cacaaacgtc acccgtcctg gcgagccctc
      241 ctcgcaacgt ccttcatgcc gcaatttgct ccagatcgct tcggccacat caagggttta
      301 cctttgacgg caccgggcca acgcatcggc ctcctcggcg gctcgttcaa tcctccccac
      361 cttgcccatc gccaaatttc cgaaatcgtt ctcaagcgac tgggacttga ccagatctgg
      421 tggctggtga cgcccggcaa tcccttgaag gcaccgggcg acctcgcccc gctcgccgag
      481 cggctggtgc tgtgccgggc gatggccaaa aatccgcgca tcgtggtcac cgccttcgag
      541 cgggatctgc cggctgccta tacggccgga accctcgcct tcttgaagcg gcgcagcccg
      601 ctggtgcgct tcgtctggat catgggggcc gacaatttgg ccggcttcca ccgctggcaa
      661 cgctggcggg agatcttgac gatggttccc atcgtcgtcg tggatcgccc gggttggcgg
      721 ctcaaggcgc ttgcctcgaa ggcggcgcgc gccttcgccg cgagccaggt ccccgaaacc
      781 aaggcgcgcg gcttggtggc gatgccaccc ccggcgtgga cctttttgac cggaccgctg
      841 tccccgctgt cctccacgga cctgcgccgc aaggcgcgac agcgtcgccc ctctgttgct
      901 cgaaagacac agtcggcaga gcctgctgcc gcggctttgg gcgccagtcg acgcccgtcc
      961 gaggctgctc gggcacgcac cggtgacgct accgtttccg agccgccaca ggccgatcgt
     1021 gcgccggccc gggtcgtagg gcgcggcgag acgcggtcgc ggcgccggcc gcttaaggga
     1081 acaccgcaag cctcttgaaa gcggcggccg tcgccctcta tccttgactt gagtcccagc
     1141 cgttggggac gggatccggg gttgagggcc ggcccgggcc gaaagagccc gacagtttgc
     1201 ccaccccgat aagggtttaa tgacgaaagg accatcaaca caagatgcca accgcacttg
     1261 agggtgctcg cacgagcacg cctccggccg aactcgtgtc tcctgaacac ggctcggaag
     1321 cccttctcgc gatcatcacg gccgcgctcg acgaggcaaa ggccgaagcg gtggtggcga
     1381 ttgacatcaa gggaaaatct tccatcggcg actatatggt gatcgcctcc ggtcgctcgg
     1441 accggcacgt gggcgcgatc gccgagcaac tgcagcataa gctgaaagat gcaggctatg
     1501 gtcgcgcgcg catcgagggc cagccccagt gcgattgggt gctgatcgac gccggcgaca
     1561 tcatcgtgca cgtgtttcgg ccggaggtgc gcgagtttta taatctcgag aagatgtggt
     1621 cggccgagcg cccgagcgat ccgacgacgc actagttcgg cggtgttgtg ggcttgcgag
     1681 gtctcggcgt ccttttcgtg aaggcaggga cgctgcgtca tgcgcgtggt cattgccgcg
     1741 atcggccgtc tcaaggacgg acccgagcgg gtgctgtttg aaaggtatcg cggccggttt
     1801 gaagctgcgg gtcgacggct tggcttcgcc cccgtcgagt gcggtgaagc tgccgagagc
     1861 caggccgcga gcgtcgccaa gcgcaaaacc gaggaagccg cggcgctgcg caagttgacc
     1921 cgcgatgctg agaccgtcat cgccttggat gcggcgggaa aagcctgcac cagcgccggc
     1981 tttgccgagc tattggccaa aatccgcgac gagggcagca gggggttggc cttcgccatt
     2041 ggtggaccgg atggcctggc cgccgatatt ctgggggcgg ccaagatgcg gctctcgttc
     2101 ggagccataa cgttgccgca ccagctggcg cgcattgtgc ttgccgagca gctctaccga
     2161 gccgccacca tacttgccgg ccatccctac catcgcgcat gaatggcagg gaaatcgcgg
     2221 gccggcgcgt gccgggcgtt gcggccggac cgcgatcgat tgatttgacc gagaaagcgc
     2281 gaacaagcca tgacgagaaa caccctggcc ccggagacgg tggcggcgca ggctggcgat
     2341 gctggcgatg aggcaacggg cgccatcgtt ccgcccgtac atctcgccac aacctttgtt
     2401 cgcgatccgg acaatcaata tcgcaagggc ttcagctacg gccggcccga caatgccacc
     2461 gtgcgacagg tcgaacgggt gctggctgag ctggagggcg gcgctggcgc cctggtggtc
     2521 gcttccggca tgacggcggc caccatggcg tttttggcac tggagcggcc ggcacatgtc
     2581 gtggcgccca aggtgatgta ctggggcttg cgcgattgga tcagccggga tgccccgagc
     2641 ttcggcatcg agcccagctt tgtggatggc gccgacccgg cggcgatcgc ggcggccatc
     2701 cggcccgggg ccaccaagct cgtctggctt gaaacgcccg ccaatccgct gtggaccatc
     2761 accgacattg ccgcggcggc caagctggcg cacgc
//
LOCUS       DASTMT010000149        14065 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_5948074, whole genome shotgun sequence.
ACCESSION   DASTMT010000149 DASTMT010000000
VERSION     DASTMT010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 14065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14065
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5948074"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(392..1291)
                     /gene="sucD"
                     /locus_tag="VFR00_03220"
     CDS             complement(392..1291)
                     /gene="sucD"
                     /locus_tag="VFR00_03220"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006609294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HEU0158294.1"
                     /translation="MAILVDKNTKVLCQGITGSQGTFHTQQALAYGTQVVGGITPGKG
                     GSRHPDATLAKVPIFDTVSEAKARTGANATSIYVPPRFAADAILEAIDAEMPLIVCIT
                     EGIPVLDMVKVKRALDGSKSRLIGPNCPGVLTPEACKIGIMPANIFTAGTVGIVSRSG
                     TLTYEAVKQTTDAGLGQSTAVGIGGDPVKGTEFIDVLEMFLADRATQAIIMIGEIGGA
                     AEEDAAEFIKAEARRGRKKPMAGFIAGMTAPPGRRMGHAGAIIAGGKGRAEDKAEAMK
                     SAGIAVADSPAALGTTLAKVLQS"
     gene            complement(1293..2489)
                     /gene="sucC"
                     /locus_tag="VFR00_03225"
     CDS             complement(1293..2489)
                     /gene="sucC"
                     /locus_tag="VFR00_03225"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HEU0158295.1"
                     /translation="MNIHEYQAKELIGRYGAPVPRGAVALSVAEAVTAAQALPGPVWV
                     VKAQIHAGGRGKGRFKEAAEAAGGVRLAKSSAEVKAHAEAMLGKTLITVQTGPAGRTV
                     KRLLVEEGCEIARELYLSCLVDRAASRLAFIASTEGGMDIEKVAHDTPEKIARLTIDP
                     ASGYAPFHARKIAAALKLTGDQIGQCDKLIGALYQAFVDTDMSLLEVNPLIVTKAGKL
                     LCLDAKINFDDNALFRHKQVAELRDIDEEDPAEVEASKYDLNYVKLDGEIGCMVNGAG
                     LAMATMDIIKLYGMEPANFLDVGGGASKEKVAAACKIILRDANVKGILVNIFGGIMRC
                     DIIAEGVVAAAKEMAIKVPLVVRLEGTNVELGKDILAKSGLKIVPADNLADAAKKITD
                     AVKKAA"
     gene            complement(2595..2981)
                     /locus_tag="VFR00_03230"
     CDS             complement(2595..2981)
                     /locus_tag="VFR00_03230"
                     /inference="COORDINATES: protein motif:HMM:TIGR00004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family detoxifying hydrolase"
                     /protein_id="HEU0158296.1"
                     /translation="MAKHAITSPASRPIGPYSPGIGVGDLVFLSGQIGLEAASGKLVD
                     GGISTQARQCLENLKVLLAAAGLGFEHVLKTTIFLTDMGNFAAVNEVYRAYVREPYPA
                     RSTVAVAGLPLGAAVEIELIASRSAQ"
     gene            complement(3091..4056)
                     /gene="mdh"
                     /locus_tag="VFR00_03235"
     CDS             complement(3091..4056)
                     /gene="mdh"
                     /locus_tag="VFR00_03235"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HEU0158297.1"
                     /translation="MARSKIALIGAGMIGGTLAHLVGLKELGDVTLFDIVEGVPGGKS
                     LDIWQAGAVEGFDAKFKPANAYAEIEGADVIIITAGVPRKPGMSRDDLLGINLKVMEQ
                     AGAGIKKYAPNAFVIVITNPLDAMVWALQKACGLPKQKVVGMAGILDTGRFRAFLAEE
                     FDVSVEDVSALVLGGHGDDMVPIARYSTVAGIPLPDLVRMGWTTQAKLDAIIERTRKG
                     GGEIVNLLKTGSAFYAPASAAIQMAESYLKDKRRVLPCATYLNGEYGVKGLYVGVPVI
                     IGANGVEKIVEIDLNAAERQMFLKSVESVKGLIEASKKIAPQLAS"
     gene            4334..5722
                     /locus_tag="VFR00_03240"
     CDS             4334..5722
                     /locus_tag="VFR00_03240"
                     /inference="COORDINATES: protein motif:HMM:NF012946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEU0158298.1"
                     /translation="MPQTTPDKYCIIGAGPSGLAAAKTFLERGIAFDCLEREADIGGL
                     WNIATQSGIVYETTHLVSCISSTGFDDLPMLDADYPEYPSHERVLSYFRDYIAEFGLA
                     PHIELGKAVTKLAPLGGGGWQVSVAGEAQPRRYRGVVIASGHHDIARMPTYPGTFAGE
                     IMHSRRYKSPLQVRDKRVLVVGCGNSAADVVRDAVHGRSKVYLSLRRGYWFVPKFILG
                     FPTGDVLSWAEIVPLPRRLKRWLFQASLWLLQGPPSRYRMPQPDHAIDEAHPTMSDEI
                     PRLVAHGSLIVKPEIARYEGERVLFSDGSTETVDLIVFATGYRPVLPFLDEDIVFLRE
                     GRTRFVHHVFHPDYDSLFAVGLVQANGSMWRLADYQAQAIASSIIAERCAPGRWARFR
                     AGLARVQERAHFVASERHRLEVNYFDYRRKLKRLNRSFGHVRKLRLHASPAGEAPALR
                     RPTTPATPGQVP"
     gene            complement(5688..6809)
                     /gene="zapE"
                     /locus_tag="VFR00_03245"
     CDS             complement(5688..6809)
                     /gene="zapE"
                     /locus_tag="VFR00_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HEU0158299.1"
                     /translation="MTVLDDYRALIAAGALEADAAQAEEAAKLDRLVEALRHWQPKRP
                     GLPGLFDRSTPAPKGRYIFGAVGRGKTMLMDLFYAATKFKPRRRLHFHAFMSEVHDLI
                     AAARKRVPGDPIPEVARTLAGRARLLCFDELHVTDIADAMILGRLFDALFEAQVVLVA
                     TSNVPPADLYKDGLNRQLFLPFIDLLMVKMEIDELRAAKDFRLAKLSGKSLYFWPADA
                     RARAEMDRLWSALTSGHPADAVAIDVKGRRLHVPQAAMGIARFSFAELCERPLGTLDY
                     LALAHNFHTIMIDSIPAMTVAQRSVARRFVTLIDTLYDARVCLIASAAAEPRGLLPAN
                     EAGLLVDRTVSRLIEMRSEAYLEGRGERIRAPAPALPAW"
     gene            7132..7707
                     /locus_tag="VFR00_03250"
     CDS             7132..7707
                     /locus_tag="VFR00_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013419891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU0158300.1"
                     /translation="MSDQPFREGSIRKLWPTDAGLFREHLLRLDKESRRLRFAHSVSD
                     AFIEEYASRMDDFGSMVFAYFLAGRVRGAAELRRLGDVWGIEAEAAFSVERMHQDNGI
                     GSELMGRVVRAARNRGIRRLYMSCLAENGRMQAIARKHDAELRFELGEVIGEIVPQTP
                     DYVSQISEAAEDRVGFMIAVLELHHRLLRAA"
     gene            complement(7718..8551)
                     /locus_tag="VFR00_03255"
     CDS             complement(7718..8551)
                     /locus_tag="VFR00_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009764215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU0158301.1"
                     /translation="MSSDYEAQYNNRARVPEHATINARWSEASIDYRRKAAGQLDIAY
                     GPGERHRYDFFPGRADPAPLVVYIHGGYWQRGDRKDYAFLARELNAAGLAVALPSYSL
                     CPAVSVMEIVGELRQFLAALWQTTRVYPLVIGHSAGGHLTAAMLATDWRQAGDVPADL
                     VRAGYAISGIFDLPPLVGTSLNVALGLSAESARAASPMFWPPPPKARVFAAAVGGLES
                     EEFLRQSQAIAHTWRQAGVTAEDVVVANTNHFTIVDELARPTSPMFKSVVALARTIAA
                     R"
     gene            9012..10328
                     /gene="hmgA"
                     /locus_tag="VFR00_03260"
     CDS             9012..10328
                     /gene="hmgA"
                     /locus_tag="VFR00_03260"
                     /EC_number="1.13.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012709494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004411 - homogentisate 1,2-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HEU0158302.1"
                     /translation="MQWSAATPAGYMPGFGNDFETEALPGALPVGQNSPQRTAYGLYA
                     EQLSGSPFTAPRGSNERSWLYRIRPSVKHTAKFRKLALPYWKTAPAIAEHDRPIGQLR
                     WGPVPLPDAPLTFITGLRTMTTAGDVNTQAGMAAHVYLVTQSMPNDYFYNADGEMLIV
                     PQLGRLSFFTEFGVIEVAPGEICVIPRGVKFKVDLLEGPARGYVCENYGGKFTMPDRG
                     PIGANCLANARDFKTPMAAFEDREEKGRLTVKWCGSFNVCELGHSPLDVVAWHGNYAP
                     YKYNLRHFSPVGAIAFDHPDPSIFTVLTAPSETAGTANVDFVIFPERWAVAEHTFRPP
                     WYHMNIMSEFMGLIYGVYDAKPEGFIPGGMSLHNCMLPHGPDKTAYEHATGGELKPVK
                     LTNTLAFMFETRFPQHLTKFAAELETLQEDYAACWTPLEKRFTGAK"
     gene            10393..11436
                     /locus_tag="VFR00_03265"
     CDS             10393..11436
                     /locus_tag="VFR00_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013532316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEU0158303.1"
                     /translation="MKVATYRNGTRDGALIIVSRDLRKMVPAASVIAGLETLQQLLDD
                     WDDLLLKVERVYAELNEIAEVERTPRFEVEDFQAHRTTAPLPRAYQWADGSAYLNHVE
                     LVRRARGAEMPASFWTDPLMYQGGSDSFLGATEAIALADEAWGIDLEGEVAVVTGDVP
                     TGSSAAEAAERVRLIMLANDVSLRHLIPGELAKGFGFFHGKPSTAFSPVAVTPDELGS
                     AWRDGKLHLALLAHLNGKLIGRPNAGKDMAFSFPELIAHAAKTRPLGAGSIVGSGTVS
                     NRGEGEPTKPIELGGVGYTCLAELRTVETILEGRPHTPFLKFGDRVRIEMLDAAGHSI
                     FGAIEQEITRYVR"
     gene            11472..12419
                     /locus_tag="VFR00_03270"
     CDS             11472..12419
                     /locus_tag="VFR00_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009451625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU0158304.1"
                     /translation="MASFASTGDVSEKNISFTEIGRDLYAFTAEGDPNSGIIIGDDGV
                     IVVDAQATPVMAGLVIERVRQVTDKPIKYVVLSHYHAVRVLGATAYGANEILASEACR
                     AMIAERGQEDWASEYQRFPRLFRAAESIPGLTWPSLTFRQRMTLYLGKRRVELCQIGR
                     AHTAGDIVAYVPDANVVFSGDIVEYKSACYCGDAHFDDWPGTLERLAALHADALVPGR
                     GDALVGADRVAEALVLTRDFLRDTYEPVKRGVARGASLKECFEAASAAMRPKYASWPI
                     FEHCQPFNIARAYDEAQGIDTPRIWTAERDRTMWSELQG"
     gene            12465..13772
                     /locus_tag="VFR00_03275"
     CDS             12465..13772
                     /locus_tag="VFR00_03275"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0158305.1"
                     /translation="MQTVPQPIVASWLKGRLPFFYGWVILACLCCCGFARQGPAVATL
                     SIFVEPMTSEFGWSRTALSGAVSLGGILAALTSPLIGPLLDRRGARIMLCAAVLLTGI
                     STMLLSLIQSIVGFYLLFCIARMNFAGPFDIGIYGAVNSWFLTRRAIVNAIVGLAQMV
                     GLVLMPLIAHFAMRADGWRGGWLAVGSTVLLIGLLPAWLVLVRRPEDLGLLPDGGRPP
                     PEPAGAAAGARVAVAEPQFSRAQALRTPAFWLLSLYTVAVYPVQAGISLHQAPHLLES
                     GLSPTVAATVVSTFSFAAAVAGLGFGLVARRMGLPTLLALSGVALGTSAMLMLKIAVA
                     WQAYLAACSFGAGIGGILTLLPLAWADYFGRASFGAIRGVALSLQVTAQAAGPLLSGL
                     LRDASGSYVLSLAAFAALSFFSALIATLVRPPRPPAKRLPAAP"
     gene            13827..>14065
                     /locus_tag="VFR00_03280"
     CDS             13827..>14065
                     /locus_tag="VFR00_03280"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU0158306.1"
                     /translation="MSDVRGIRFRHRRSADQDRSAPQHHPIIIVGAGPVGLVAALELA
                     RKGHRSILLEKKTNLSDGSRAICWAKRSLEIMDRLA"
BASE COUNT         2329 a         4656 c         4537 g         2543 t
ORIGIN      
        1 tgtgcctgca tctgttcgat gtaggcggca ttggcaccct gcaggaccga ggtctgcgca
       61 aaagcttggt tctttggctg gcgggtcatg ggctgagaac gtcgtcgggt tggaaggaac
      121 ggctggcagc atcgcacacg cacgtgacca aacgcctacg cgggcggcgc ctacctagca
      181 cattgctgga tatagtacat gccgcggccg gcttttagac ggggtgtgcc gcgcgccgcc
      241 caagcacgcc agggtcgcgc tcgccctgag ccgctcgcag gagccaagtg caccaaatct
      301 cggcaacgcc cgcgccggtg cgagccggcc tggcgcacga ctgcgcccaa actgccggcg
      361 tggacaccag gggcgctttt gtgatggggc gctaactctg cagcaccttg gccagcgtgg
      421 tgccgagggc ggcgggacta tcggcgacgg caatgccggc cgatttcatc gcctccgcct
      481 tatcttcggc gcggcctttg ccgccggcga tgatggcacc tgcgtgtccc atgcgccggc
      541 ctggcggcgc cgtcatgccg gcaataaagc ccgccatcgg cttcttgcgg cctcgccttg
      601 cctctgcctt gatgaactcg gccgcatcct cctcggccgc cccgccgatc tcgccgatca
      661 tgatgatcgc ctgggtcgcc cgatcggcta ggaacatttc caacacatcg ataaattccg
      721 tgcccttgac gggatccccg ccaatgccca cggccgtcga ctggcccagt cccgcatccg
      781 tggtttgctt cacggcttcg taggtgagcg tgcccgagcg cgaaacaatg ccgaccgtgc
      841 cggccgtgaa gatgttggcc ggcataatgc cgatcttgca ggcctccggc gtcagcacgc
      901 cggggcaatt cgggccgatc aagcgcgact tggagccatc gagcgcccgc tttaccttca
      961 ccatatcgag caccggaatg ccttccgtga tgcagacgat caagggcatc tctgcatcga
     1021 tggcctccag gatggcgtcg gcggcaaagc ggggaggaac ataaatggag gtggcattgg
     1081 ccccggtgcg cgccttcgcc tcgctcacgg tgtcgaagat cggcaccttg gccagcgtcg
     1141 catccgggtg ccggctgccg cccttgcccg gggtgatgcc gccgacgacc tgagtgccgt
     1201 aggcaagtgc ctgctgggta tgaaaggtgc cctggctgcc ggtgatgccc tggcacagga
     1261 ccttggtatt tttatcgacg agaatggcca tctcaggctg cctttttcac ggcgtcggtg
     1321 atcttcttgg cggcgtcggc aaggttgtcg gccggcacga tcttcaagcc cgacttggcg
     1381 agaatgtcct tgccgagctc cacgttggtg ccctccagcc gcaccaccag cggcaccttg
     1441 atcgccatct ccttggccgc ggccactacg ccttcggcga tgatatcgca gcgcatgatg
     1501 ccgccgaaga tgttgaccag aatgcccttc acgttggcgt cgcggaggat gatcttgcaa
     1561 gccgcggcta ctttttcttt cgaagcgcca ccgcccacgt cgaggaagtt ggcgggctcc
     1621 atgccgtaga gcttgatgat atccatggtg gccatggcca ggcccgcacc attgaccatg
     1681 cagccgattt cgccgtcgag tttgacatag ttgagatcgt acttggaggc ttcgacttcg
     1741 gcgggatctt cctcgtcgat gtcgcgcaac tcggccacct gtttgtgacg gaagagcgca
     1801 ttgtcgtcga aattgatctt ggcatcgaga cagagaagct tgccggcctt ggtcacgatc
     1861 aacggattga cctcgagcag gctcatgtcg gtatcaacaa aggcctggta gagcgcgcca
     1921 ataagcttgt cgcattgccc gatttgatcg cccgtgagct taagggctgc ggcgattttg
     1981 cgcgcgtgga acggcgcata gcccgaagcc ggatcgatcg tcaggcgcgc gattttctcc
     2041 ggcgtatcat gcgccacctt ttcgatgtcc atgcctcctt cggtggaagc gataaaggcc
     2101 aagcgtgacg cggcccgatc gacaaggcag gagaggtaga gttcgcgcgc gatctcacag
     2161 ccttcctcga ccagcaggcg cttgacggta cggccggcgg gaccggtctg cacggtgatg
     2221 agggtcttgc ccagcatggc ttcggcgtgg gccttcacct cggccgacga cttggccaag
     2281 cggaccccgc ccgccgcctc ggcggcttcc ttgaaccggc ccttgccgcg gccgccggcg
     2341 tggatctgtg ccttcaccac ccacaccggc ccgggcaggg cttgggcggc ggtgaccgcc
     2401 tcggcaacgc tgagggcgac ggcgccgcgc ggcaccgggg cgccgtaacg gccgatcagc
     2461 tctttggcct gatattcgtg gatgttcatg gcagccccgg cgattgactg tgtgcggtgc
     2521 cctgtcgcgg cactggcata ccatatacca gtgccgcccg ggccgtcagc aggggcaatg
     2581 ccgcctgctg agcctcattg tgcactgcga gaggcgatga gttcaatctc cacggctgct
     2641 cccaagggca ggccggcaac ggcgacggtg gagcgcgccg gatagggttc gcgcacgtag
     2701 gcccgataga cctcgttgac ggcggcaaaa tttcccatgt cggtcaagaa aatggtggtc
     2761 ttgagcacgt gctcgaaacc aaggccggcc gccgccagca gcaccttgag gttttcaaga
     2821 cactgccggg cttgggtgga gatgcctcca tcgaccaact tgccgctggc ggcttccagg
     2881 ccgatctgcc cggacaggaa aacgagatcg cccacgccga tgcccgggga ataggggccg
     2941 attggccggc tcgcaggcga cgtgattgcg tgttttgcca tagtggccgc tcctcctgcg
     3001 ctccgctgtg attttcggcc gcgcccgcgc gctttgggcc caagacggct ccgcctcggc
     3061 ggccctcgcg ggctcaccga acctcggcca ctaggaggcc aactgcgggg caatcttttt
     3121 cgaggcctcg atcaagccct tcaccgactc gaccgacttt aagaacatct gtcgctcggc
     3181 ggcgttgagg tcgatctcca cgatcttctc tacgccattg gcgccgatga tgacgggaac
     3241 gccaacgtaa agacccttca cgccgtactc gccgttgaga taggtcgcac acggcagcac
     3301 acggcgcttg tccttgagat aactctccgc catctgaatt gccgcggatg cgggcgcata
     3361 gaacgccgaa ccggtcttca gcaggttgac gatttcgcca ccacccttgc gtgtgcgctc
     3421 gatgatggcg tcgagcttgg cctgcgtggt ccagcccatg cgcaccagat cgggcagcgg
     3481 tatgcccgca accgtggagt aacgcgcgat cggcaccatg tcgtcgccat gcccaccgag
     3541 caccaacgcc gagacgtctt ccaccgagac gtcgaactcc tcggccagga aggcgcgaaa
     3601 gcgaccggta tcaagaatgc cggccatgcc aaccactttc tgcttcggca ggccacaggc
     3661 cttttgcaag gcccaaacca tggcgtcgag cggattggtg ataacgatga caaaggcatt
     3721 gggtgcatac ttcttaatgc ccgcgccagc ctgctccatc accttgagat tgatgccgag
     3781 cagatcgtcc cggctcatgc ccggtttgcg cgggacgcct gccgtgatga taatgacgtc
     3841 ggcaccctcg atctcggcat aggcattggc gggcttgaac ttcgcatcga agccctcgac
     3901 cgcaccggcc tgccagatgt caaggctctt gccgccgggt acgccctcga cgatatcgaa
     3961 gagggtgaca tcaccaagtt ccttgagacc caccaagtgc gccagcgtgc caccgatcat
     4021 gcccgcgccg atgagtgcaa tcttgctacg cgccatactg ttgctcctcg ctggcttgcg
     4081 gagacgccac cctttcaaga ccaaggccgg cattgtgcgc cgcgtggggg gctcgcgcgg
     4141 ttggttaacc cgaaacccgc ggcggttcaa gctggaggag ggcggatgcc gccgagcgct
     4201 cgcggctgac ccggcgggcc ccggccccgc tcccagcgcg cttgcgtcac cttgtcgagc
     4261 gccgtgccgc cccctacctg gctatcgttc ccggcgcgca acgggagacc ctagcccaga
     4321 gtttagcgca tagatgccgc aaacgacccc agacaaatat tgcatcatcg gcgccggccc
     4381 ctcggggctg gctgcggcca agacctttct cgagcgcggc atcgccttcg actgtctgga
     4441 gcgggaggcc gatatcggcg gcctctggaa tatcgccacc caaagcggca tcgtttacga
     4501 gaccacgcac cttgtgtcct gcatcagctc gacgggcttc gacgacctgc ccatgctcga
     4561 tgcggattac cccgaatatc cgagccacga gcgcgtgctc tcctacttcc gcgactatat
     4621 cgccgagttt ggcctggccc cccatatcga gcttggcaag gcggtgacga agcttgcccc
     4681 gttgggcggc ggaggctggc aggtgagcgt cgccggcgag gcccaaccgc ggcgctaccg
     4741 cggggtagtt atcgccagcg gtcaccacga cattgcgcgc atgcccacgt atcctgggac
     4801 ctttgccggc gaaatcatgc attcgcggcg ctacaaaagc cccctgcagg tgcgtgacaa
     4861 gcgcgtcctg gtggtgggct gcggcaactc cgccgccgac gtggtgcgag atgccgtgca
     4921 cgggcgctcg aaggtgtact tgagcctgcg ccggggttac tggttcgtgc caaagttcat
     4981 cctcggcttc ccgaccggtg acgtcctctc ctgggcggaa atcgttcccc tgccgcgccg
     5041 cctcaagcgc tggttgttcc aggcgagcct gtggctcctg caggggccgc cctcgcgcta
     5101 ccgcatgccg cagcccgacc acgcgatcga cgaggcccat cccaccatga gcgacgagat
     5161 ccctcgtctg gtcgcgcacg gcagcctgat cgtcaagccg gagattgcgc gctacgaggg
     5221 tgagcgcgtg ctctttagcg atggcagcac cgaaacggtc gatctcatcg tattcgcaac
     5281 cggctatcgg ccggtgttgc cattcctcga cgaggacatc gtctttcttc gcgagggccg
     5341 aacccgcttc gtccaccacg tcttccatcc cgactatgac agcctgtttg ccgtgggctt
     5401 ggtgcaagcc aatggcagca tgtggcggct cgccgactat caggcgcagg ccatcgccag
     5461 cagcatcatc gccgagcgat gcgcgccggg ccgctgggcc aggtttcgtg ctgggctggc
     5521 gcgcgtccag gagcgcgcgc acttcgtcgc ctccgagcgg caccggctcg aggtgaacta
     5581 cttcgactat cgccgtaagc tgaagcggct caaccgcagc tttggccatg tgcgaaagct
     5641 tcggctgcac gcgagcccgg cgggggaggc gccggcgctg cgtcggccta ccacgccggc
     5701 aacgccgggg caggtgccct gatgcgctcg ccgcggccct ccaggtaggc ctccgagcgc
     5761 atttcgatga gccgcgagac cgtgcgatcg acgagcaagc ccgcctcgtt ggcgggcaac
     5821 agacccctgg gctcggccgc cgccgaagcg atcagacaca cgcgcgcatc ataaagcgta
     5881 tcaatcaaag tgacgaagcg ccgcgccacg ctgcgctgcg ccaccgtcat ggccggaatg
     5941 ctgtcgatca tgatggtgtg gaaattgtgc gccagcgcca gatagtcgag cgttccaagc
     6001 ggccgttcgc acagctcggc aaacgagaag cgggcaatgc ccatcgcggc ctggggcacg
     6061 tggagccggc gccctttcac gtcgatcgca accgcgtccg cgggatgccc gctcgtcaac
     6121 gcgctccaca gccggtccat ttcggcgcgc gcccgcgcat cggcgggcca gaaataaagc
     6181 gacttgcccg acagcttggc gaggcgaaaa tccttagcgg cccttagctc gtcgatctcc
     6241 atcttgacca tcaggagatc gatgaatggg agaaacagtt gccggttgag gccgtccttg
     6301 tagaggtccg ccggaggcac attcgacgtc gccaccagca ccacttgggc ctcgaacagg
     6361 gcgtcgaaca gccgcccgag aatcatggca tcggcgatgt cggtgacgtg cagttcatcg
     6421 aaacaaagca gccgggcgcg gccggcaagc gtgcgcgcga cctctgggat cggatcgccc
     6481 ggcactcgct tgcgcgctgc ggcgatgagg tcgtgcactt cactcatgaa ggcatgaaaa
     6541 tgcaggcgtc gtcgcggctt gaatttggtg gccgcgtaga acaggtccat gagcatggtc
     6601 ttgccgcgcc cgacggcacc aaaaatgtag cgccccttgg gcgccggcgt ggaccgatcg
     6661 aagagcccgg ggagccccgg tcgcttcggt tgccagtggc gcagggcctc aaccagcctg
     6721 tcgagttttg ccgcttcctc cgcttgcgcc gcgtcggcct caagggcgcc ggcagcgatc
     6781 agcgcgcgat agtcgtcaag caccgtcatc gcgcctcggc tatcgcggcc aggagccctt
     6841 ggtcaagcca tcgcccgcac gccttcccct caggccgcgg gcccgatcgc ccgccttgcc
     6901 catttttccg gcagggccgg ccggggcggt atgcggggcc atgcggggag ttatgtggca
     6961 gcgcagcatc aggtctgaaa gcttgtggta taacactttt ttgaagtcat ggtgcggtca
     7021 tggcgggcct ctattttgcg atgcaaaaga gttctgacaa cgatgcccgg cccccgaagg
     7081 cggcgcgcag gctcgcgccg acatcgggcc aggtcagtca aggagatgag catgagcgat
     7141 cagcctttcc gcgagggcag catccgcaag ctgtggccga ccgacgcggg gctctttcgt
     7201 gagcacttgc tgcggctcga caaagagagc cgacggctgc gctttgcgca ttcggtttcg
     7261 gacgccttca tcgaggagta cgcgagccga atggacgatt tcggcagtat ggtctttgct
     7321 tattttctcg ctggccgggt gcgcggcgcc gccgagctgc ggcggctggg cgacgtctgg
     7381 gggatcgagg cggaagccgc gttttccgtg gagcgcatgc accaggacaa cggcatcggc
     7441 tcggagctta tgggacgggt tgtccgcgcc gcccgcaacc gcggcatccg ccgtctctat
     7501 atgagctgcc tggcggagaa cgggcgcatg caggcgattg cccgcaagca cgacgccgag
     7561 ttgcgattcg aattgggcga ggtgatcggg gaaatcgtgc cgcaaacccc cgactacgtg
     7621 tcccagatca gcgaagccgc cgaagaccgc gtcggcttca tgatcgccgt cctcgagttg
     7681 caccaccgtt tgctgcgggc cgcctgagcc cgaggaatta acgcgcagcg atggtacggg
     7741 ccagcgccac caccgatttg aacatcgggc tcgtcggtcg cgccagctcg tcgacgatag
     7801 tgaagtgatt ggtgttcgcc accaccacgt cttccgcggt gacccccgcc tgacgccagg
     7861 tgtgcgcgat ggcctggctt tggcgcagga actcttcgct ttcgagcccg cccacggcgg
     7921 cggcaaacac gcgcgccttg ggtggcggag gccagaacat cgggctggca gcacgcgcgc
     7981 tctccgcgct gaggcccaag gccacgttga ggctggtgcc caccagcggc ggcagatcga
     8041 agatgccgct gatggcatag cccgcccgca ccaggtcggc aggcacgtcg ccggcctgcc
     8101 tccagtcggt ggccagcatc gccgcggtca agtgcccgcc ggcggaatgg ccgatcacga
     8161 gggggtagac gcgcgttgtc tgccacagcg ccgccaggaa ctggcgcagc tcgccgacga
     8221 tctccatgac gctgacggcc gggcacaggg aataggaggg caaggccacg gcgaggccgg
     8281 cagcgttgag ctcacgggca aggaaagcat aatccttgcg gtcgccgcgc tgccaatagc
     8341 cgccgtggat gtagaccacc agcggggcgg gatcggcgcg ccctggaaag aaatcgtagc
     8401 ggtgccgctc gccgggacca taggcgatgt cgagctggcc cgccgccttg cggcgatagt
     8461 cgatagaggc ctccgaccag cgcgcattga tcgtggcgtg ctcgggaacc cgggcccgat
     8521 tgttgtactg ggcctcgtaa tcgctgctca tctcacctgc ccaaatcagt ttttcaggtc
     8581 tggcccccgc acctgcccgc cgcccgactt tgccccggat aggcgcgcac cttaagcccc
     8641 agcacggggg atgtcatcct gcccgtcccg ccaggcacgc ggagccggcc ggcggcgcgc
     8701 caggcgcaac atgcgccggc cgcgtcatcg gccaagcctc gagaaacgat aggggcgggg
     8761 cgctcgggtg acgccagtgc gcttgccggc gccatcgcca aacgcgcgcc gtcgcctgcg
     8821 ctttgcgctc gtcgccaggg aggcgtaaag atggcacgcc atgcggcggt ttggcgctct
     8881 gtggaaaagc aaacggggcg cgcgctcttc gccgccggca atggacgcac gctggtcggc
     8941 aaggccgcgc agcaagatgg tgagttagct cgatgccaga cggtagcaaa acccgatcgg
     9001 acgcccatgc catgcaatgg agcgcggcca cccccgccgg ctacatgccc ggcttcggca
     9061 acgacttcga aaccgaggcg ctccccggcg cgctgccggt cgggcaaaac tcgccgcagc
     9121 ggaccgccta tggactttac gccgagcagc tctccggctc gccattcacc gcgccgcgcg
     9181 gcagcaacga gcgatcctgg ctctatcgta tccggccctc ggtcaagcac acggcaaaat
     9241 tccgcaagct ggccttgccc tattggaaga cggcaccggc catcgcggag catgatcggc
     9301 cgatcggtca gttgcgctgg ggcccggtgc cgcttcccga cgcgccgctc accttcatca
     9361 ccggtctgcg caccatgacg acggcgggcg acgtcaacac ccaagccggt atggctgcgc
     9421 acgtctatct cgtcacccag tcgatgccca acgactactt ctacaacgcc gacggcgaga
     9481 tgctgatcgt cccacaactg ggccggctca gcttctttac cgagttcggt gtcatcgaag
     9541 tcgcgccggg cgaaatctgc gtcattcccc gcggcgtgaa attcaaggtc gacctgttgg
     9601 aaggtccggc gcgcggctac gtgtgcgaga attatggcgg caagttcacc atgcccgatc
     9661 gggggccgat cggcgccaat tgccttgcca atgcgcggga tttcaagacg ccgatggcgg
     9721 ccttcgagga tcgcgaagag aaaggccgtc tgaccgtcaa atggtgcggc agcttcaacg
     9781 tctgcgagct cggccattcg ccgctcgacg tggtggcctg gcacggcaac tacgccccat
     9841 acaagtataa cctgcgccac ttctccccgg tcggggcgat cgccttcgac catcccgatc
     9901 cctcgatctt caccgtgctg acggcgccgt ccgagacggc gggcactgcc aacgtcgatt
     9961 tcgtgatttt tccggagcgt tgggcggtgg cggagcacac cttccggccg ccctggtacc
    10021 atatgaacat catgtccgag ttcatgggct tgatttacgg tgtctacgat gccaagccgg
    10081 agggcttcat ccccggtggg atgagcctgc acaattgcat gctgccgcac gggcccgaca
    10141 agacggccta cgagcacgcc accggcggtg agttgaagcc ggttaaactg acgaacacgc
    10201 tcgcctttat gttcgagacc cgctttcccc agcacttgac caagtttgcg gccgagctcg
    10261 agaccctgca ggaggactat gccgcctgct ggacgccgct cgagaagagg ttcacggggg
    10321 cgaaatgaaa cggcgcccgc accccaggcc cgcgcggggc gatgacggcc tgcggcgcgc
    10381 tggggccaca ccatgaaggt cgcaacctat cgcaacggca cgcgcgatgg cgccctcatc
    10441 atcgtctcgc gcgacctgcg caagatggtg cccgccgcct cggtgatcgc gggcctcgag
    10501 acgctgcaac agctgctgga cgactgggac gacctgttgc tcaaggtcga acgtgtctat
    10561 gccgagctca acgaaattgc cgaggttgag cgcacgccac gcttcgaggt cgaggatttt
    10621 caggcccacc ggacgacggc accgctgcca cgcgcctacc agtgggccga tggttcggca
    10681 tacctcaatc acgtcgaact ggtgcgccgg gcacgcggcg ccgagatgcc ggcgagcttc
    10741 tggaccgacc cgctgatgta tcagggcgga tccgacagct tcttgggggc gaccgaggcg
    10801 atcgcgctgg cggacgaggc ctggggcatc gatctggagg gcgaagttgc ggtcgtcacg
    10861 ggcgacgtgc cgacggggtc cagcgcggcg gaggcggctg agcgcgtccg cctcattatg
    10921 ctggccaacg acgtgtcgct gcgccacctg atccccggcg aactcgccaa gggcttcggt
    10981 ttttttcacg gcaagccctc gaccgccttt tcccccgtgg cggtgacccc ggacgagctg
    11041 ggctcggcct ggcgcgacgg caagctccac cttgcgctgt tggcacacct caacggcaag
    11101 ctcatcggcc ggccaaatgc cgggaaagat atggcgtttt catttcccga gctcatcgcg
    11161 cacgcggcca agacgcgccc gctcggcgcc ggcagcatcg tcggttccgg cacggtgtcc
    11221 aatcggggcg agggggagcc gaccaagccg atcgagctcg gcggcgtcgg ctatacctgt
    11281 cttgccgagt tgcgcacggt ggagaccatc ctggagggtc gcccgcacac gccttttctc
    11341 aagttcggtg accgcgtccg catcgaaatg ttggatgccg ccggtcactc gatcttcgga
    11401 gcaatcgagc aagagatcac acgctacgtg cgctagaggg tgcgcggcgg caagcaagga
    11461 gcacttggcg aatggcgagt tttgcctcca ctggtgacgt gagcgagaaa aacatcagct
    11521 ttacggaaat cggccgcgac ctttatgcct tcaccgccga aggcgatccc aacagcggca
    11581 tcatcattgg cgatgacggc gtcatcgtag tcgacgcgca ggcgaccccg gtgatggcag
    11641 ggctggtgat cgagcgcgtg cgccaggtca cggataagcc gatcaagtat gtggtgctgt
    11701 cgcactatca cgccgtgcgc gtcttgggcg cgacggccta tggggccaac gagatcctgg
    11761 cctcggaggc ctgccgggcc atgatcgccg agcgcggcca ggaggactgg gcctccgaat
    11821 atcagcgttt cccccggctg tttcgtgccg ccgagtcgat accgggcctc acctggccaa
    11881 gcctgacctt tcgccagcgc atgacgctct atctcggcaa gcgccgcgtc gagctatgcc
    11941 agatcgggcg cgcacacacc gccggcgaca ttgtggccta tgtgccggac gccaacgtgg
    12001 tgttctcggg cgacatcgtc gagtacaagt ccgcttgcta ctgcggcgat gcgcacttcg
    12061 acgactggcc gggcacactc gagcgcctcg cagcactgca cgccgatgct ctggtgcccg
    12121 ggcggggaga cgcccttgtc ggtgccgacc gggtagcgga ggccctggtg ctgacgcgcg
    12181 acttcctgcg cgacacctat gagcccgtca agcgcggggt ggcgcgcggg gctagcctca
    12241 aggagtgctt cgaggccgcg agcgcggcca tgcgtcccaa atatgcgagc tggccgatct
    12301 tcgagcattg tcagcccttc aacatcgcac gcgcctatga cgaggcgcag ggcatcgata
    12361 ccccgcgcat ctggacggcc gagcgcgacc ggaccatgtg gagcgaactt cagggttgat
    12421 gggccgtttg caatctgcgc cggcaccgcg gacagaaggg aaacgtgcaa accgtcccac
    12481 aaccgatcgt tgcctcatgg ctcaaggggc ggctgccgtt cttctatggc tgggtcatct
    12541 tggcctgcct gtgctgctgt ggcttcgcgc gccaaggccc ggcggttgcc accctttcca
    12601 tttttgtcga accgatgacg agcgagtttg gctggtcgcg cacggcgctg tctggcgccg
    12661 tgtcgctcgg cggtatcctc gccgcgctca cgtcgccgct gatcggtccg ctgctcgatc
    12721 ggcgcggcgc gcgtatcatg ctgtgcgccg ccgtcctgct gaccggcatc tcgaccatgc
    12781 tcttgtcgct aatccaatcg atcgtcggct tctacctgtt gttctgcatc gcgcgcatga
    12841 attttgccgg gccgttcgat atcggcatct atggcgccgt caacagctgg ttcttgaccc
    12901 gccgcgccat cgtcaacgcc attgtcggcc tggcccagat ggttggtctt gtcctgatgc
    12961 cgcttatcgc gcattttgcc atgcgcgcgg acggctggcg cggcggctgg ctggccgtcg
    13021 gcagcacggt gttgctgatc ggtctgctgc cggcctggct ggtgctggtg cgtcgccccg
    13081 aagatttggg tttgctgccc gatggcggcc ggcccccgcc ggagccggcg ggcgccgccg
    13141 cgggggcccg ggtcgccgtg gccgagccgc agttctcgcg tgcgcaagcc ctgcgcacac
    13201 cggccttttg gctgctgtcc ctctataccg tggcggtcta tccggtgcag gccgggatca
    13261 gcttgcacca ggccccgcac ctgttggaaa gcggtctctc gccgacggtc gccgccaccg
    13321 tcgtctcaac cttttctttt gcggcggcag tggcgggact tggttttggt ctcgtcgccc
    13381 gccgcatggg tctgccgacc ttgttggcgc tcagcggtgt cgccctcggc acctccgcca
    13441 tgctgatgct gaaaatcgcc gtggcctggc aggcctatct cgccgcctgc agcttcggcg
    13501 ccggcatcgg cggcatcctc actctgctgc cgctcgcctg ggccgattat ttcgggcggg
    13561 cgagcttcgg cgcgatccgg ggcgtggcgc tctccctgca ggtcaccgcc caggccgcgg
    13621 ggccgctcct ttcggggctg ctgcgcgatg cgtcgggcag ctacgtgctg tcattggcag
    13681 ctttcgctgc tctatcgttt ttcagcgcgc tcatcgcaac cctggtgcgc ccgccgcggc
    13741 cgccggcgaa acggcttccc gcggccccgt gatggacgcc gcattcggcg gggtgtgtta
    13801 ggctggagtt ggcattcgag ggaccgatga gcgacgttcg cggcattcgc ttcagacatc
    13861 gacgctcggc ggatcaagat cgcagcgcgc cgcagcatca tcccatcatc atcgtcggcg
    13921 ccggcccggt cgggctggtc gccgccctcg agctcgcccg caaaggccac cggtccatcc
    13981 tgctcgagaa aaagaccaac ctatccgacg gcagtcgcgc gatttgctgg gccaagcggt
    14041 cgttggagat catggatcgg ctcgc
//
LOCUS       DASTMT010000150         5723 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_737889, whole genome shotgun sequence.
ACCESSION   DASTMT010000150 DASTMT010000000
VERSION     DASTMT010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 5723)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5723)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5723
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_737889"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            71..475
                     /gene="flgD"
                     /locus_tag="VFR00_03285"
     CDS             71..475
                     /gene="flgD"
                     /locus_tag="VFR00_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012318685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HEU0158307.1"
                     /translation="MQVASTTPGAGASAPSSPARAAKSATVDYNQFLQLLIAQLKNQD
                     PTKPMDSAQFISQLASFSNVEQSIQTNAKLDALMTSSALSQAEGMIGRRITSADGETS
                     GTIAAVRIVSGGAVALLDNGSELPLEAGVTLF"
     gene            478..744
                     /locus_tag="VFR00_03290"
     CDS             478..744
                     /locus_tag="VFR00_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014761126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliQ"
                     /protein_id="HEU0158308.1"
                     /translation="MNQADALDIVQSALWTVIVASSPAVGAAMAIGIIIALLQALTQV
                     QEITLTFIPKILAILIIASLTAPFIGTVIFTFAEQVYARIEVGF"
     gene            815..2902
                     /gene="flhA"
                     /locus_tag="VFR00_03295"
     CDS             815..2902
                     /gene="flhA"
                     /locus_tag="VFR00_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012336148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HEU0158309.1"
                     /translation="MAASTAGVTAVERSDGQDVGFAIGIVFILTVLFLPLPATFIDLG
                     LAFSIAFSVLILMVALWIQKPLDFSAFPTVLLIATLLRLALNIATTRLILSHGNEGST
                     AAGHVIAGFANFVMSGDFVIGTIVFVILITVNFLVITKGATRIAEVGARFTLDAIPGK
                     QMAIDADLNAGLIDEKEAMRRRRELEEESAFFGSMDGASKFVRGDAIAGIIITAVNVF
                     GGIVIGVLRHDMSPADAATVFTKLSVGDGLVTQIPALIVSLAAGLLVSKGGTRGSAEK
                     AVFGQLGNYPRALSIASMVMVVLALAPGLPFLPFAMLAAMMGFIAYAIPKRRAELKAA
                     ETAKAQAVAEAAAEEARNSVKESLKTVEIELCLGKQISSSLLKSHNELAQRVGKMRRR
                     FAKQYGFVVPEIKLSDDLAIPAKSYQIKIHGTIAASAELNIGDVLVVTGDGAKPDLPS
                     EETREPAFGMRAVWVAETFAGTLKRDGFNPIDTMSVLLTHVREVIRNNLAQLLSYKDM
                     RSLLERLEPEYRRLLDEIAPSQISYSGLQAVLKLLLAERISIRNLHLILEAIAEIAPH
                     VRRAEQLAEHVRTRMSQQICGDLTDGGVLKVLRLGNRWDLAFHQSLKRDAKGDIVEFD
                     IDPRLIEQFGKEASKVIRGLMDQGHQFVLVAAPDARPYVRMVMERLFATLPVLSHVEI
                     ARGVEIKALGTIS"
     gene            2915..3670
                     /locus_tag="VFR00_03300"
     CDS             2915..3670
                     /locus_tag="VFR00_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HEU0158310.1"
                     /translation="MSAITANTVLLTFLLFCRIGGCLMLMPGFASTRVPVQVRLLIAV
                     AATLALAPLLLPVLSAALPALPAPTVLALLVSETTLGALIGLMGRLFFLALQFMASAA
                     AMFVGFNASAGVPLEDNEPVPAMTALVTLTATLLFFLTDQHWEVLRALVASYMALPVS
                     DALAADFTLAKLADALSSAFVLALQISSPFIIYALLINFMIGLANKFTPQIPVYFIAV
                     PFVLAGGFFILYLTIGDALRFFMLGFMGWLARG"
     gene            3676..4089
                     /locus_tag="VFR00_03305"
     CDS             3676..4089
                     /locus_tag="VFR00_03305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0158311.1"
                     /translation="MQDRTKKARRLTTIQTELMQIEQWKLSHLQAQLTELEREQEALI
                     AALNGGSRLHELFFAAHARRLGRLAQAMTQLRGEREQQALRLLEETGRARMAASLAQR
                     AECVSRRASERSELDRVIEDFAGHALQASGKIAGP"
     gene            4123..4659
                     /locus_tag="VFR00_03310"
     CDS             4123..4659
                     /locus_tag="VFR00_03310"
                     /inference="COORDINATES: protein motif:HMM:NF021638.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod-binding protein"
                     /protein_id="HEU0158312.1"
                     /translation="MGITPPSDIVLGSMLAADPDKYRAAVERLRQFAADRPPPTVAAA
                     PAAPVALAPRAAVSAARRPAASDAFGQLEAFVLQTFIENMLPSNAEVAFGKGTAGAVW
                     KSMLAEQLAKQMALSGQVGLARRLAEGFEGRALPAGEASRAPAIAAFGAGALPPASPY
                     LQGHLIDALKNEETASIP"
     gene            4685..5119
                     /locus_tag="VFR00_03315"
     CDS             4685..5119
                     /locus_tag="VFR00_03315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0158313.1"
                     /translation="MPSAAALSPPSSSAAVANETGAGGQLRLAAIIAHLSEVVGEETA
                     HLRRGAAVDLKVANDRKSQALLELTRALRQVEPSVARDAAVATRLAELRARLEQNQAI
                     LKLHLEAVREVSNIVCAVIRHAESDGTYSPSIRKHGPEPWCD"
     gene            5107..5586
                     /locus_tag="VFR00_03320"
     CDS             5107..5586
                     /locus_tag="VFR00_03320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0158314.1"
                     /translation="MVRLIISGVWVCLVTLAALYGTVWWQTAARSPAPAVAEAADPRQ
                     AAIESLKTRMISVPVVGDGAIHGYVMAQFIFNVNAAIAKRLPVKADMLLLDEAFKTIY
                     AGDVVDFRHFKKQDLAGLSKGIAENANKRLGVHLVEDVLIQELNYIAKDQVRGGRRL"
BASE COUNT          950 a         1914 c         1803 g         1056 t
ORIGIN      
        1 gcttgatcga agtgtcgacg gccattcgcg acggcctcaa ttgaggccag gcaaaaggcg
       61 agccccatcc atgcaagtag ccagcacgac cccgggcgcc ggtgccagcg ccccgagcag
      121 tcccgccagg gcggccaaat ccgcaaccgt cgactacaac cagtttttgc agctgctgat
      181 cgcgcagctg aagaaccagg atccgaccaa gccgatggac tcggcgcagt tcatctccca
      241 gcttgccagc ttttccaacg tcgagcagag cattcagacc aatgccaagc tcgatgcact
      301 gatgacgtcc tcggccttgt cgcaagccga gggcatgatc ggccggcgca ttaccagcgc
      361 cgacggcgaa acgagcggca cgattgcggc ggtgcggatt gtctccggcg gcgccgtggc
      421 gctgctcgat aacggttccg agctgccgct agaggccggc gtcacgctgt tctgatcatg
      481 aatcaagccg acgccctcga tatcgtgcaa tcggcactgt ggacggttat cgtcgcctcc
      541 agccccgcgg ttggcgccgc catggccatc ggcataatca ttgctttgct gcaggccttg
      601 acccaggtgc aagagatcac cttgaccttc atccccaaga tcctggcgat cctgatcatt
      661 gcctcgttga cggcaccttt catcggcacg gtcatcttca ccttcgccga acaggtctac
      721 gccaggatcg aggtcggctt ctagccgatc ttgagcacct tcgcgcaagc ttcatcgggc
      781 attattgcta agaaggcgtg gggcgcatcg gctcatggcg gcaagcacgg cgggggtgac
      841 ggcggtcgag cgtagtgacg gccaggatgt gggctttgcc atcggcatcg tcttcatcct
      901 gaccgtgctg tttttgccgc tgccggcgac cttcatcgat ctcgggctgg ccttctcgat
      961 cgccttttcc gtgctgatct tgatggtcgc gctgtggatc caaaagcctc tcgacttttc
     1021 cgcctttccg accgtgctgc tgattgccac gctgctgcgg cttgccctca acattgccac
     1081 cacccggctc attctttcgc acggcaatga gggatccacc gcggccggtc acgtcatcgc
     1141 gggcttcgcc aattttgtga tgagcggcga tttcgtgatc ggcaccatcg tcttcgtcat
     1201 cctgatcacc gtcaatttcc tggtcatcac caaaggtgcg acccgcattg ccgaggtcgg
     1261 tgcgcgcttc acgctcgacg ccattcccgg caagcagatg gccatcgacg ccgacctgaa
     1321 cgcgggcctc atcgacgaga aggaagccat gcgccgccgg cgcgagctcg aggaggaaag
     1381 cgcctttttc ggctccatgg acggcgcctc caagttcgtg cgcggcgatg ccattgccgg
     1441 catcatcatc acggccgtca acgtgttcgg cggcatcgtc atcggcgtcc tgcgtcacga
     1501 catgtcgccg gccgatgccg ccaccgtctt caccaagttg tcggtgggtg atgggctcgt
     1561 cacccagatc ccggccctga tcgtgtcgct ggccgccggc ctgctcgtct ccaagggcgg
     1621 cacgcggggc tcggcggaga aggcggtgtt cgggcagctc ggcaactacc cgcgcgcgct
     1681 ctcgatcgcc agcatggtca tggtcgtgct cgccttggcg ccgggcttgc cgtttctgcc
     1741 gtttgccatg ctggcggcca tgatgggctt catcgcctat gccatcccca agcgccgagc
     1801 cgagctgaag gcggccgaaa ccgccaaggc gcaggcggtg gccgaggcgg cggcggaaga
     1861 ggcgcgcaac tcggtcaagg aatccttgaa aaccgtggaa atcgagctct gcctcggcaa
     1921 gcagatctcg agctctctcc tgaagtcgca caacgaactc gcccagcgcg tcggcaaaat
     1981 gcggcgccgg tttgccaagc agtatggctt cgtcgtaccc gaaatcaaac tcagcgacga
     2041 cctggcgatt cccgccaaga gctaccagat caagatccac gggaccatcg cggccagcgc
     2101 cgaactcaac atcggcgatg ttctcgtcgt aacgggcgat ggcgccaaac ccgatctgcc
     2161 ctcagaggaa acgcgcgagc cggccttcgg catgcgcgcc gtgtgggtgg cggaaacctt
     2221 cgccggtacg ctgaagcgcg acggcttcaa cccgatcgac accatgtcgg tcttgctgac
     2281 gcacgtgcgc gaggtcatcc gcaacaacct ggcgcagctg ctctcctaca aggatatgcg
     2341 tagcctgctc gagcgtctcg agccggagta ccgccgcctg ctggatgaga ttgcgccatc
     2401 gcagatttcc tattccggct tgcaggccgt gctcaaactg ctgctggcgg agcgcatttc
     2461 cattcgcaac ctgcacctga tcctcgaggc cattgccgag attgccccgc acgtgcggcg
     2521 cgccgagcag ctcgccgaac acgtgcgcac ccgcatgtcg cagcagatct gcggcgacct
     2581 gaccgatggc ggtgtgctca aggtgttgcg gctcggcaac cgctgggatc tcgccttcca
     2641 ccaaagcctg aagcgcgatg ccaagggcga tatcgtcgaa ttcgacatcg acccgcggct
     2701 gatcgagcag ttcggcaagg aggcctcgaa ggtgatccgc ggcttgatgg atcaggggca
     2761 ccagttcgtc ctggtcgccg cgccggatgc ccgcccgtac gtacgcatgg tgatggaacg
     2821 gctgttcgcc accttgcccg tgctgtcgca cgtggaaatt gcgcgcggcg tggagatcaa
     2881 ggcccttggc accatctcct gaacggccgg cggcatgagc gcgatcacgg cgaataccgt
     2941 gctcttaact ttcctgctgt tttgccggat tggcggctgc ctcatgctga tgccggggtt
     3001 cgcctcgacg cgggtccccg tgcaggtgcg cctgctgatt gccgtggcgg ccaccttggc
     3061 gctggcgcct ttgctgctgc cggtcctttc tgcggctctc ccggcattgc cggcgccgac
     3121 cgtgctcgcc ctgcttgtct cggaaacgac gctcggcgcc ctgatcggct tgatgggccg
     3181 cctgttcttt cttgccctgc agttcatggc gagtgcggcg gccatgttcg tcggctttaa
     3241 cgcctcggcc ggcgtgcccc tcgaagacaa cgagccggtg ccggccatga cggccttggt
     3301 gacgttgacg gcgacgctcc tgttcttttt gacggatcaa cattgggagg tgctgcgcgc
     3361 gctcgtcgcc tcctatatgg cgctgccggt cagcgacgca ctcgccgccg acttcaccct
     3421 ggccaagttg gccgacgcgc tgtcgagcgc cttcgtgctg gccctgcaaa tcagcagtcc
     3481 cttcatcatc tacgcactcc tgatcaactt catgatcggc cttgccaata agttcacgcc
     3541 acagatcccc gtctatttca tagccgtgcc attcgtgttg gcgggcggct tctttatcct
     3601 ctacctcacg atcggggacg cgctgcgctt cttcatgctg ggcttcatgg gctggctggc
     3661 gcgaggttga gagccatgca ggatcggaca aaaaaggcgc gtcggctcac aaccattcaa
     3721 accgagctca tgcaaatcga gcaatggaag ctctcccatt tgcaagcgca actgacggag
     3781 ctggagcgcg agcaggaggc gctgatcgcg gcgctcaacg gcgggagccg cctgcacgag
     3841 ctttttttcg ccgcgcacgc ccgccgcctg ggtcgcctgg cgcaggcgat gacccagctc
     3901 cgcggcgaac gggagcagca ggccttacgc ctcctcgagg agacgggtcg cgccaggatg
     3961 gcggcgagcc tggcgcagcg cgccgagtgc gtttcgcggc gcgcgagcga acgcagcgag
     4021 ctcgatcggg tgatcgagga ctttgccggc cacgcgctgc aagcctccgg caagattgcc
     4081 gggccatgat tgcctttgct tcggcgtagc gggacagcca gcgtgggcat cacacctccc
     4141 tccgatatcg tgcttggcag catgctcgcg gccgatcccg acaagtatcg ggccgcggtg
     4201 gaacgcctgc ggcagttcgc agccgaccgg ccccccccaa ccgtcgcggc cgcgcctgcc
     4261 gcccctgtcg cgcttgcccc gcgcgccgcc gtttccgcgg cgcgtcggcc ggcggcgagc
     4321 gacgccttcg gccagctcga agccttcgtg ctgcagacct tcattgagaa tatgctgccg
     4381 agcaacgcgg aggtcgcctt cggcaagggt acggccggcg ccgtctggaa atcgatgctg
     4441 gccgagcagc ttgccaagca gatggcgctg agcggccagg tcgggctggc gcggcgtttg
     4501 gccgagggtt tcgagggccg tgcgctgccc gccggcgagg cgagccgcgc cccggccatc
     4561 gcggcctttg gcgccggcgc gctgcccccc gcctcgccct atctgcaggg gcacctgatc
     4621 gatgccctga aaaacgagga aaccgcctcc atcccatagc ccaacctggg gagccgaaag
     4681 agccatgccc agcgccgccg cgctcagccc gccctcgagt tctgccgccg tcgccaatga
     4741 gacgggagcc ggcggacagc tgcggcttgc ggcaatcatt gcgcatctga gcgaggtcgt
     4801 cggcgaggaa accgcccact tgcgccgcgg cgctgccgtc gacctcaaag tcgccaacga
     4861 ccgcaagagc caggcgctgc tggagttgac gcgggcgctg cgccaggtcg agccatcggt
     4921 ggcgcgcgat gccgcggttg cgacgcgcct cgccgagcta cgcgcgaggc tcgaacagaa
     4981 ccaggcaatc ctgaaactcc acctggaagc ggtgcgcgag gtttccaaca tcgtctgcgc
     5041 cgtcatccgg cacgccgaat cggatggcac ctactcgccc tccattcgca agcacggccc
     5101 cgagccatgg tgcgactgat catcagcggc gtttgggtct gcctcgtcac gctcgccgcg
     5161 ctttacggca cggtgtggtg gcagaccgcg gcgcgctcac cggcacccgc ggtggccgag
     5221 gccgccgatc cgcggcaggc ggcgatcgaa tcgctcaaga ccagaatgat cagtgtgccg
     5281 gtggtcgggg acggtgccat ccacggctac gtgatggccc aattcatctt caacgtgaat
     5341 gcggcgatcg ccaagcgttt gccggtgaag gcggacatgc tgttgctgga tgaggccttc
     5401 aagaccattt atgccggcga tgtcgtggat tttcgtcact tcaagaaaca ggatctggcc
     5461 ggcctctcca agggaatagc cgagaatgcc aacaagcgct tgggtgtgca cctcgtcgag
     5521 gatgtgctga tccaggagct caactacatt gccaaagatc aggtgcgcgg cggccgccgg
     5581 ctctaggccg agggcacctg gtcgcccgca gccgtcgggg gcctggcggc ctgcgtcgcc
     5641 tggcccgcat cgggtgcaag cgcccgcacc atggcctgga aggcgtcggg gctcgcgaca
     5701 tcacgtgggc cctgacgcaa gac
//
LOCUS       DASTMT010000151         8292 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_3362086, whole genome shotgun sequence.
ACCESSION   DASTMT010000151 DASTMT010000000
VERSION     DASTMT010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 8292)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8292)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8292
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3362086"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..976
                     /locus_tag="VFR00_03325"
     CDS             <1..976
                     /locus_tag="VFR00_03325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0158315.1"
                     /translation="VESAKSPVKSEAAKTALEAARALVAKGHVRAGREQLLALAGTSR
                     EWNWPGLGLAIIHHLDDPVACLVYQQYPDLIRGPLRANVTPTWWRDYPNLIKAAQAAA
                     DEDLVDTLASRYAVRIAWNRIWHPTRPRGPDETTTSELAAYYQAIRDRDASQFSRRAS
                     NVLTRIPAYASFNQSLLLRHNELARLLFVRSLEQYLLVPEAVQDLVEGSNIHVMMLAY
                     RVLALPDPRARALAAANLEILLGTLLRPLHRKTRLAAFEALANAARHDAAIARRIHAR
                     AREALKLPDKKYPKPELIGLIARTLAAYPALASPAERPLVYRPSGVAA"
     gene            973..2571
                     /locus_tag="VFR00_03330"
     CDS             973..2571
                     /locus_tag="VFR00_03330"
                     /inference="COORDINATES: protein motif:HMM:NF016330.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HEU0158316.1"
                     /translation="MIVLVDYASPSLLDSTREETTVGLATNLRRRVRFYGRVREHGFV
                     LRIALRALGEVIWSNDMWFGQSDLLDPVITVHRDRIFFEAFSQDQSAYASLILDPSLF
                     EIMGDVVSGTTNIDFTAWLWGALGEMRTSRSTIFRIGPEGFDVRTSSAGGRFEKKVDL
                     PESWVRGFLQVQAAMGMPGTRLRVKPIDLLTPVRFLRYAKAKVSPRAIRYEFEPGQDA
                     RLVLEPWERATVLEGAEHNYSEFKTTRVWGRRRLKLIEGLLPFAESVDIYLKGRALPS
                     FYAVKLPGMTFLLGLTGWSGAGFMGTGGFDLLAEAAAADDGALEPAVELLRARQHASI
                     KDVATALNLDVQGAARVLVRLCRQGRAMFDVESQRYRHRELFETPADEATYFPPDRRR
                     ELAAALLAEGRVRVKSAAAEETKKVRSFKDPTSGEAKQKIVREITYRDWRIIGAAGDA
                     DPVEIVINDLGSIIFGRCTCPHFAEHLLNQGPCEHMLALFKASEAARSDRPSSTPVDA
                     SSSVGPRKQPSRWEVDGAQAGASE"
     gene            2786..4135
                     /locus_tag="VFR00_03335"
     CDS             2786..4135
                     /locus_tag="VFR00_03335"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: protein motif:HMM:NF012307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase family protein"
                     /protein_id="HEU0158317.1"
                     /translation="MHPVVSLGALWVLFLLVRAAVRALRRWRQKRRWQRAPTRDVSAL
                     AETGAAAFKQEVIKTGGPLTPEHRRLVLRDNRLLPKPPPDPNARRWPPPPKVKYLSER
                     EADRLFGPSLRTRNRDVMDLAVDEAQLKRYGLPLWRTEEDVAAALELSPGQLRHLAQH
                     RFTDRAPHYVTFAVPKRSGGERLIHAPKRRLKRVLRALDRLLVSKLPKSEHAHGFVKG
                     RSIASNAAPHVGKAVVLHFDIKDCFPSLHFGRVRGLLLALGYSYPVAASLAVLMTEAP
                     RQPVATDGKLYHVPIGPRVCVQGAPTSPGLCNAILTRLDHRLAGLARRHGFAFTRYAD
                     DLSFSGNDLGKVAKFLALVPRIVAAEGFVVNAGKTRVLRRGRRQAVTGVVVNTTLGLS
                     RQERRKLRAALHHQARASAEAADGAERARLTGKLAYLFMLNRGQAAALGWGRVREMT"
     gene            complement(4192..4473)
                     /locus_tag="VFR00_03340"
     CDS             complement(4192..4473)
                     /locus_tag="VFR00_03340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0158318.1"
                     /translation="MRTFFLVALFLLAACGVASAQSAGKREGSPSPSSAVQEPPLSAA
                     EIRERSAQYLTDCVNDWDKGTHMSKKDWTRTCRRVVQRRVDFMLQQQQK"
     gene            4578..5087
                     /locus_tag="VFR00_03345"
     CDS             4578..5087
                     /locus_tag="VFR00_03345"
                     /inference="COORDINATES: protein motif:HMM:NF025292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin-like domain-containing protein"
                     /protein_id="HEU0158319.1"
                     /translation="MRIARWLVAALALVSVAEPALAQSAKTLREQLIGTWTFVVAEVT
                     AADGSTTFPFGETPKGVLIFTPDGHFAQIHVASDLPKIASNNRLTGTPEEYKAINQRS
                     LSVFGTYTVDEDKKTVTYQITASTFPNWQGVSQTRTIEALTADEFKNTNPSVSGGRGS
                     ASNLYKRAK"
     gene            5201..5452
                     /locus_tag="VFR00_03350"
     CDS             5201..5452
                     /locus_tag="VFR00_03350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0158320.1"
                     /translation="MAKKLRKSEKLDLILSELAALRADLKTLLQRGVAPDKAPGPAKA
                     GGAKKAAKPKKAAKAPTRPVLVEAAESDAPAAGLASRIA"
     gene            5659..7215
                     /locus_tag="VFR00_03355"
     CDS             5659..7215
                     /locus_tag="VFR00_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002713310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HEU0158321.1"
                     /translation="MLIFEWILGILLGAVLLSVLARRLGVPYPALLALGGTVLAFIPG
                     APRLVLDPELALALFVAPVLLDAAYDASPRDLKDNWLPVTSLVLIAVLVTTGAVAVVA
                     RALTGMPWPAAIALGAIVAPPDAAAASAVLRQVPLPHRIVTILEGESLLNDASALLVY
                     RVAVTAMMVGGISFTQAAPTLLLSVVGSLIAGPILALAYMRLVTALDQTGDAPSSIIL
                     QFIGTFGVWLLADRLGLSGILTIVAYAITIARRAPATTPARLRVPSYAVWETAVFVLN
                     VLAFVLIGLQIGPIFEMLAPEQRLRYLLVAGAVLATVILVRFAWVMSYNWAVRRHIRR
                     YGYHPRRPMPAPTVKSGLLVSWCGMRGIVTLVAALALPNGDGGAAFPYRDLIVFVAFC
                     VVLGTLVLQGFTLRPLLIKLALEDDDPVGREVARARTAAYEAALAAISDDRSAVAKAI
                     RVELQAALLQSNGGGTDPAADLESDRLRLRIAAAARQAVLGMRNSGDIGDTAFHRLEE
                     EIDRLELSAS"
     gene            7253..8023
                     /locus_tag="VFR00_03360"
     CDS             7253..8023
                     /locus_tag="VFR00_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020096147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU0158322.1"
                     /translation="MARIFVTGSSTGLGLMAAELLIEQGHRVVLHARNEARAKELMAA
                     APGAEAVVVGDLASLRQTRALATQVNRLGRCDAVIHNAGVGYREPRLETEDGLEHIFA
                     INVLAPYVLTLLIERPDRLVYLSSGMHHGVAPDFEDLLWRKRRWQGAGAYAESKFYDT
                     VLASAVARHWPAVNANALEPGWVPTRMGGPGAPGDMAKAHLTQAWLAVSEDALARSSG
                     EYFYHQALRAPNPLARDEATQENLLRECARLSGVPFKP"
BASE COUNT         1280 a         2783 c         2782 g         1447 t
ORIGIN      
        1 cgtggagagc gccaaatcgc cggtcaaatc ggaggcggcc aagactgccc tcgaagctgc
       61 ccgcgccctg gtggccaagg ggcacgtgcg cgcggggcgc gagcagctgc tggcgctcgc
      121 cggcacctcg cgggaatgga actggccggg gctcggcctg gcgatcattc accacctcga
      181 cgatccggtc gcctgcctcg tctaccaaca gtatcccgac ttgatacgcg gtccgctgcg
      241 agccaacgtc acgcccacct ggtggcgcga ctatcccaat ctcatcaaag cggcgcaggc
      301 ggcggccgac gaggacctcg tcgatacgtt ggcgagccgc tacgcggttc gcattgcctg
      361 gaaccggatc tggcatccca cccgcccgcg cgggcccgat gagaccacca cctccgagct
      421 cgccgcctac taccaggcca ttcgcgaccg cgatgccagc cagttttcgc ggcgcgcctc
      481 caatgtgctg acccgcattc ccgcctacgc gagcttcaac cagagcctgc tgctgcgcca
      541 taacgaattg gcgcggctct tgttcgtgcg ctccctcgag cagtacctgc tggtgcccga
      601 ggccgtgcag gacctggtcg agggctccaa tatccacgtg atgatgctgg cctatcgggt
      661 tctggcactt cccgatccgc gggcgcgcgc cttggcggcc gccaatctcg agatcctgct
      721 cggcaccttg ttgcggccgt tgcatcgcaa gacgcggctg gcggcgttcg aggcgctcgc
      781 caacgcggcc cggcacgatg cggcgatcgc ccggcgcatt catgcgcggg cgcgcgaggc
      841 gctcaagctg cccgacaaga agtatcccaa gcccgagctc attggcctga tcgcgcgcac
      901 gctggcggcc tatcccgccc ttgccagccc ggcggaacgg ccgctggtct atcgaccttc
      961 gggggtggcg gcatgattgt cttggtcgac tacgcctcgc cctcgctgct cgactccacg
     1021 cgcgaggaaa cgaccgtcgg gctcgccacc aacctgcgcc ggcgcgtgcg cttttacggg
     1081 cgcgtgcgcg agcatggctt cgtgctgcgc atcgcgctgc gcgccttggg cgaggtgatc
     1141 tggtcgaacg atatgtggtt cggtcagtcc gatctgctcg accccgtcat cacggtgcac
     1201 cgggaccgca ttttcttcga ggccttcagc caggaccagt cggcttacgc gtcgctgatc
     1261 ctcgatccct ccctattcga aatcatgggc gacgtcgtca gtggcacgac caacatcgac
     1321 tttaccgcct ggctctgggg agcgctgggc gagatgcgca ccagccgctc gacgattttt
     1381 cgcatcgggc cggaaggctt cgatgtccgt acctcctcgg ccggcggccg cttcgaaaaa
     1441 aaggtcgacc tgcccgagag ctgggtcagg ggctttctac aggtgcaggc ggccatgggc
     1501 atgccgggca cgcgtctcag ggtcaagccg atcgatctgc tgacgcccgt gcggtttttg
     1561 cgctacgcca aggccaaggt gtcgccgcgg gcgatccgct acgagttcga gccgggtcag
     1621 gatgcacggc tggtgctcga accgtgggaa cgcgcaacgg tgctcgaggg cgccgagcac
     1681 aactacagcg agttcaagac cacgcgggtg tggggacggc gccgcctcaa gttgatcgag
     1741 ggcttgctgc cctttgccga gagcgtcgac atctatctca agggccgtgc gctgccgagc
     1801 ttctacgccg tcaaattgcc gggcatgaca ttcctgctcg ggctcaccgg ttggtccggg
     1861 gcgggcttta tgggcacggg gggatttgat ctcctggccg aggccgctgc cgccgacgac
     1921 ggcgccctcg agccggccgt cgagctgttg cgcgcccgac agcacgcctc gatcaaggac
     1981 gtggccacgg cgctgaacct cgatgtgcag ggagccgcac gcgtgctcgt gcgcctgtgt
     2041 cggcagggcc gcgccatgtt cgatgtggaa agccagcgct accgtcatcg cgagctgttc
     2101 gagacgccgg ccgacgaggc gacctatttt cccccggatc gccggcgcga actggccgca
     2161 gccttgctgg ccgagggtcg ggtgcgcgtg aagagcgcgg ccgcggagga gaccaagaag
     2221 gtgagatcct tcaaggatcc gacctccggc gaggccaagc agaagatcgt gcgcgaaatc
     2281 acctaccgcg attggcgcat catcggcgcg gcgggcgatg cggaccccgt cgagatcgtg
     2341 atcaacgacc tcggcagcat cattttcggg cggtgcacgt gcccgcattt tgccgagcat
     2401 cttttgaacc aggggccgtg cgagcacatg ctggccctgt tcaaggcgag cgaggcggcg
     2461 cgctccgacc gcccctcgag cactcccgtc gacgcctcca gcagcgtcgg cccgcgcaaa
     2521 cagccgtcgc gctgggaggt tgacggcgcg caggcgggag cgtcagaatg agccctcttt
     2581 gtgcagtccc cgggtttggc aggaccttcc agctttccat accacggtgc ttcgccgtgg
     2641 tggcttcctg tccttatcac atcgccacgg ttccccacgt acgaaacgag gggagaccac
     2701 gggtccaaag cctcgttagt ggtgggcctt ccaacccggg actgcacaat tctctgggtg
     2761 ctttgccgct gcaagcgggc gtggcatgca cccggtagtc tccctgggcg cgctttgggt
     2821 tttgttcctg ctggtgcggg ccgccgtgcg cgcgctgcgt cgctggcggc aaaagcggcg
     2881 ctggcagcgc gcgcccacgc gcgacgtttc cgcgcttgcc gaaacgggtg ccgccgcctt
     2941 caagcaggag gtcatcaaaa ccggaggtcc gctgacgccg gagcatcgcc ggctcgtgct
     3001 gcgtgacaat cgcctgctgc ccaaaccccc gcccgatccc aatgcccgtc gctggccgcc
     3061 gccgccaaag gtcaaatatt tgagcgagcg cgaggccgat cggctgttcg gcccctcttt
     3121 gcgcacgcgc aaccgtgacg taatggacct cgccgtcgac gaggcgcagc tcaagcgcta
     3181 tggcttgccg ctgtggcgga cggaagagga cgtggctgcg gcgctcgagc tgtcgcctgg
     3241 gcagttgcgg cacttagcgc aacaccgctt caccgacagg gcgccccact acgttacctt
     3301 cgcggtgccc aagcggtcgg gcggtgagcg cttaatccat gcgccgaaac gccgcctcaa
     3361 gcgtgtgctg cgggcgctcg accggctgct ggtatcgaaa ttgccgaaga gcgagcatgc
     3421 gcacggcttc gtcaagggac gctcgatcgc cagcaatgcc gccccgcacg tgggcaaggc
     3481 cgtggtcctg cacttcgaca tcaaggactg tttcccgagc ctgcatttcg ggcgggtgcg
     3541 tgggctgctg ctggcgctgg gctatagcta tcccgtcgcc gcgtccctgg cggtgctgat
     3601 gacggaggcg ccgcgccagc cggtggcgac cgacggcaaa ctctaccacg tgccgatcgg
     3661 gccgcgcgtg tgtgtgcaag gggcgccgac cagccctggc ttgtgcaacg ccattctgac
     3721 gcggctcgac caccgcttgg ccgggctcgc ccgccggcat ggttttgcct ttacccgcta
     3781 cgccgatgat ctgagctttt ccggcaatga tctcggcaag gttgccaagt tcctggcgct
     3841 agtgccccgc atcgttgccg ccgaaggctt tgtcgtgaat gcggggaaaa cccgcgtctt
     3901 gcggcggggc cggcgtcagg ccgtgacggg tgtcgtcgtc aacacgacgc tgggattgtc
     3961 aaggcaagag cggcgcaagc tgcgcgcggc gctgcatcac caagcccggg ccagcgccga
     4021 ggcggcggac ggcgccgagc gggcgcgcct gaccggcaag ctcgcctatc tctttatgct
     4081 caaccgtggc caggccgcgg ccttgggctg ggggcgcgtc cgcgagatga cctgagcggg
     4141 cccctgagtg ggctttgtgc cggggcaagc gttgcgcgcc ggcgaccgcg cctacttctg
     4201 ctgctgctgc agcatgaaat cgacgcgccg ctgcaccaca cgccggcagg tgcgcgtcca
     4261 atcctttttg ctcatgtgcg tgcccttgtc ccagtcgttg acgcagtccg tcaggtactg
     4321 ggcgctgcgt tcacgaattt ccgcggccga gagcggcggc tcttgcacgg ccgacgacgg
     4381 ggaagggctg ccttcgcgtt tgcccgccga ttgggcagag gcgacgccgc acgccgcgag
     4441 gaggaagagc gcgacaagga aaaacgttcg catacccaca acacgccacc aataaagaga
     4501 ggccgccaac cccgtgctgg cgctcagccc atgctcttac tgtattattg cgcccgagac
     4561 taggcatgga ggacagcatg cgcattgctc gatggctggt ggcggccttg gccctggtga
     4621 gcgtggccga gcccgcgttg gcacagtcgg ccaaaacctt aagggagcag ctgatcggca
     4681 cctggacctt cgtggtggcc gaagtgaccg cggccgatgg cagcacgacg tttcccttcg
     4741 gcgagacgcc caagggcgtt ctcatcttca ccccggatgg gcatttcgcg caaatccatg
     4801 tcgccagcga ccttcccaag atcgcctcca acaatcgcct gaccggcact ccggaagagt
     4861 ataaggccat caaccagcga agcctgtcgg tctttggcac ctatacggtc gatgaagaca
     4921 agaagaccgt cacctaccag atcaccgcca gcacgtttcc caactggcaa ggcgtgtccc
     4981 agacgcgcac cattgaagcg ctgactgccg acgaattcaa aaacaccaat ccctcggtga
     5041 gcggcggacg gggctcggca tcgaacctct acaagcgggc aaaatagcgg ggaatgccga
     5101 cggcatggtg cgagtgtagg gggcgcgctc tggcaggctg cgggcaatcg cgctatcgtt
     5161 tcgatcccct ttgggtcgcg ccaaccggca ggagcctgtc atggccaaga agttgcgtaa
     5221 gagcgagaag ctcgatctca tcttgtcgga gctcgcggcg ttgcgcgccg atctcaaaac
     5281 cctgctgcag cgcggcgttg cacctgacaa ggcgcccggc ccggcgaagg ccggaggggc
     5341 caaaaaggcc gccaaaccca agaaggcggc caaggcgccg acacgacccg tgctggtgga
     5401 ggccgccgag agcgacgcgc cggcggcagg cctcgccagc cgcatcgcct gaacgccgac
     5461 cgcgattcgc gcgggtggcg cgcgaggatg gttcccgcgc tgccacctgg gaagggctga
     5521 ggggaaggcg gcgatgcctt accgctttgc ctgcggccgt ctgggccgcc accacgatgg
     5581 tcgcaaccgc cgcgcgggtc ggcaacgcgc ccggccggca ccgttgcctc gttgcggcag
     5641 acgtgatcaa agccaggcat gctcatcttc gaatggatcc tcggcattct cttgggcgcc
     5701 gtcctgctgt cggtgttggc gcgccgcctc ggcgtgccct atcccgcgct gttggcgctc
     5761 ggcggcacgg tcctggcctt cattcccgga gcgccgcggc tggtgctcga tcccgagctg
     5821 gcgttggccc tgttcgtcgc cccggtgctg ctcgatgcgg cctatgacgc ctccccgcgc
     5881 gacctgaaag acaactggct gcccgtcacc tccctggtct tgatcgccgt gttggtgacg
     5941 acgggagccg ttgcggtcgt cgcgcgggca ttgaccggaa tgccctggcc cgccgccatc
     6001 gcgcttggcg ccatcgtcgc gccgcccgat gccgccgccg ccagcgccgt tttgcgccag
     6061 gtcccgcttc cccatcgcat cgtcaccatt ctcgagggcg agagcctcct caacgatgcc
     6121 agtgccctgt tggtctaccg cgttgccgtg acggcgatga tggtcggcgg catcagcttt
     6181 acgcaggctg ccccgaccct gctgttgagc gtcgtcggca gcctgattgc cgggccaatc
     6241 ctggcgctcg cctatatgcg actggtcacc gccttggacc aaaccggcga cgcgccgagc
     6301 tcgatcattc tccagttcat cggcacgttt ggcgtctggc tgctggccga tcgtttgggt
     6361 ctctccggca ttttgacgat tgtggcctac gccatcacca tcgcccggcg cgcgccggcc
     6421 accacgccgg cgcgcctgcg cgtgccctcc tacgccgtct gggaaacggc ggtcttcgtc
     6481 ctcaacgttt tggccttcgt gctcatcggg ctgcaaatcg ggccgatttt cgaaatgttg
     6541 gcgcccgagc agcgcctgcg ctacctgctg gtggcgggcg cggtgttggc aaccgtgatc
     6601 ctcgtgcgct ttgcctgggt gatgtcctac aattgggcgg tgcgccggca catccggcgc
     6661 tacggctacc atccgcgccg accgatgccg gcgccgaccg tcaaaagcgg gctcctcgtc
     6721 tcctggtgcg gcatgcgggg tatcgtgact ttggtcgccg cgctggcgct gcccaatggc
     6781 gacggtggag cggcttttcc ctaccgcgat ctcatcgtgt ttgtcgcctt ctgcgtcgtg
     6841 ttgggaacgc tggtcctcca gggcttcacg ctgcggccct tgctgatcaa gcttgcgctc
     6901 gaggacgatg atccggtcgg ccgagaggtg gcgcgggcgc gcaccgccgc ctatgaggcc
     6961 gcgctggcgg cgatcagcga tgatcggtcg gccgtggcca aggccattcg cgtcgagctg
     7021 caggcggcgc tgctgcaaag caatggcggg ggtaccgacc cggcggccga tctggagagc
     7081 gaccggttgc gtttgcgcat cgccgcggcg gcgcggcagg cggtgctggg catgcgcaac
     7141 agcggcgaca tcggcgatac cgctttccac cggctggagg aggagataga ccgcctcgaa
     7201 ttgagcgcga gctgacggcg gggaggctca gcctcggaca aaggagattg gcatggcgcg
     7261 catcttcgtg acgggatcct ccaccgggct cgggctgatg gcggcagaac tgttgatcga
     7321 acagggccac cgcgtcgttc tgcacgcccg caacgaggcg cgcgcgaaag agctgatggc
     7381 ggcggcgccg ggcgcggagg cggtggtggt tggtgatctc gcgagcctgc gccagacgcg
     7441 cgcactcgcg acccaagtca atcgccttgg ccgctgcgac gccgtgatcc acaacgccgg
     7501 cgtgggctac cgcgagccac gcctggagac cgaggatggg ctggagcaca tctttgccat
     7561 caacgtgctt gcgccttatg tcctgacgct gctgatcgag cgcccggacc ggctcgtcta
     7621 tctgagctcc ggcatgcacc acggcgtggc gcccgacttc gaggaccttt tgtggcgcaa
     7681 gcggcgctgg caaggggccg gcgcctacgc ggaaagcaag ttctatgaca cggtgttggc
     7741 ctctgccgtg gcgcggcact ggccggcggt caacgccaat gcgctggagc ccggctgggt
     7801 gccgacgcgg atgggcggtc caggcgcgcc gggcgacatg gccaaggcac atctgacgca
     7861 ggcctggctg gcggtgagcg aggatgccct ggcccgctcg agcggggaat atttctatca
     7921 ccaggcgctg cgcgcgccca atccgctggc gcgggatgag gcgacgcagg agaacctgtt
     7981 gcgggaatgt gcgcgcctgt ccggtgtgcc ctttaaaccc tgagcgcggg ggccggggcc
     8041 tgcgaattcc ccgaggctca acctcggcag cgacaggcca ggtcgagcgg cgttcgccgg
     8101 ccacacgtcc tggtcactgc cgcggaggca gcagaccggc agtccgatgc gcctccatca
     8161 ggcgcagggt ttgcttaatt tcgtgcaaca ggcggctgac atcgtccgtc ggctgaccgt
     8221 gacgcctcag ctcggcgacg cgcgcctctt ctgccgtcac ccgttgccgc aggtcgtcga
     8281 tgcggcgctg ca
//
LOCUS       DASTMT010000152         7339 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_3035002, whole genome shotgun sequence.
ACCESSION   DASTMT010000152 DASTMT010000000
VERSION     DASTMT010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 7339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7339)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7339
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3035002"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..306)
                     /locus_tag="VFR00_03365"
     CDS             complement(<1..306)
                     /locus_tag="VFR00_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009489444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEU0158323.1"
                     /translation="MSDCKIGLALGGGSARGWAHIGVLRALQKAGIFPDIVAGTSIGA
                     VVGGCYVAGELEALEQFARELTRRKVLGFLDFNFSGSGLITGQRLCTVLDARLKEAQI
                     "
     gene            complement(488..913)
                     /gene="hisI"
                     /locus_tag="VFR00_03370"
     CDS             complement(488..913)
                     /gene="hisI"
                     /locus_tag="VFR00_03370"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HEU0158324.1"
                     /translation="MAFLASPAFAPRGSPDEVEQGLSFQPKFDADGLIGAIVSDAATG
                     EVLMFAAMNAEALQLTLTSGFAHFWSRSRRRLWKKGEESGNLLRVSEVRTDCDQDVLW
                     LRVTVEGAGVACHTGARSCFYRKLTLPVGTAEAIGLTRG"
     gene            complement(949..1581)
                     /gene="folE"
                     /locus_tag="VFR00_03375"
     CDS             complement(949..1581)
                     /gene="folE"
                     /locus_tag="VFR00_03375"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012651511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HEU0158325.1"
                     /translation="MDDIVAKKKRPSVRLDQSRQRPTRSEAEAAVRTLIAWAGDDPQR
                     EGLKDTPRRVTEAFEEYFSGYRLDPTDVLARQFDEAGGYDDLVMLRDIRVESHCEHHL
                     APFLGVAHVAYLPNGRIVGISKIARVVEIFAKRLQTQETMTAQIADAIEAALRPRGVA
                     VLIEAEHQCMSTRGVRQPGVKTITTRFTGALEQDASYRDRFLQMVHAARP"
     gene            1816..2277
                     /locus_tag="VFR00_03380"
     CDS             1816..2277
                     /locus_tag="VFR00_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HEU0158326.1"
                     /translation="MTQLSEIYNARILELAANMPKPDRLADAHGSATAHSKLCGSTVS
                     VDLQLTDERVSAYGQTVKACLLGQAAASVMGHNIIGSTAEELRDVALKMRKMLKEGGS
                     APQGRWADLAVLEPVKDYKARHASTLLVFDAVEAALAKAEARQEPVQHAGV"
     gene            2264..2596
                     /gene="yidD"
                     /locus_tag="VFR00_03385"
     CDS             2264..2596
                     /gene="yidD"
                     /locus_tag="VFR00_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013166817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEU0158327.1"
                     /translation="MPVSSPRRARPTRAAGAAKFAAALPIHLYRWTLKPLLGHECRHL
                     PTCSEYALDAIARNGAWRGGWLALSRLCRCHPWGTHGFDPAPDLAGRRHRLAPWRYGV
                     WRAPRPQR"
     gene            complement(2650..3504)
                     /locus_tag="VFR00_03390"
     CDS             complement(2650..3504)
                     /locus_tag="VFR00_03390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0158328.1"
                     /translation="MNNPRRDRGCPPQLGRAFATGLLLSSLILGAPAFGQSSRQIIIR
                     VPPAVVAEAASQVPFPIAIEPAEAIPRNSFVRLRGLPPMAALSEGYVIGAGSWAVPIS
                     ALPDLKISLPVAAAGRSEVTILLVAVDGAVLVESKTALTVAQAANPVRERSAGPPGGA
                     SMLRAGVPVELAPPRQETTAAVPPIVPRSQSPERERAMRLLAKGNEQWEEGNVSAARL
                     FFERAADAGLAEGAMALAATFDATELGRRNVRGIKPDSKLARQWYERARQLGAGDADE
                     RLRRLGAN"
     gene            complement(3555..4730)
                     /locus_tag="VFR00_03395"
     CDS             complement(3555..4730)
                     /locus_tag="VFR00_03395"
                     /inference="COORDINATES: protein motif:HMM:NF028178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HEU0158329.1"
                     /translation="MHAILGLLALAVTALLSVPVDGYAQQLPQVKSPPPAKRPAVATP
                     AQHQALTEKLNQNTVTIVSGNPNGTYLYLAYDMSAVLDDGNELRVLPVIGKGGYQNVV
                     DVLHLRGVDLCITQSNIMSYLKKSGEFGSTIDNRLAYIAKLYNEEMHVLAGPGIKSIQ
                     DLNGKKVNFSDVGSGTQFSTRLIFELLNIKAEEVNVGQGDGYQMVKSGEIAATILIAG
                     KPTGAFGKFKLEPGMTLLPVPYTEVLEQDYFPAKLTSEDYPNLVAKGSSVDTVAIGAV
                     LASYNWARDTDRYRRVAQFIDAFFSKFGEFQKPARHSKWREANLLSNLRGWKRFPAAE
                     EWIAKNGERPASTASVAPPAIDPSIIRAQAAKAAPNDQAEQERLFREFMDWSKTQRR"
     gene            5042..6997
                     /gene="thrS"
                     /locus_tag="VFR00_03400"
     CDS             5042..6997
                     /gene="thrS"
                     /locus_tag="VFR00_03400"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEU0158330.1"
                     /translation="MSEITLTFPDGAARPYPRGITGKSLAERISKTLAKRAVAMLVDG
                     RLADLADPLLADAKVKLITRSDPEALELIRHDAAHVMAEAVQSLWPGTQVTIGPVIDN
                     GFFYDFAKAEPFHPDDLGKIEAAMRQIIARDAPFSKEFWSREEAKSYFRKKGELFKVE
                     LIDAIPAGEDIKIYKQGDWLDLCRGPHMTSTGQVGSAFKLTHFAGSYWRGDAQGQRLQ
                     RVYGTAWASDQELATYLAQLAEAEKRDHRKLGREMDLFHFQEEGPGVVFWHPKGWQLF
                     QSLINYMRRRQNAVGYREVNAPQLLDKSLWETSGHWQTFRENMFTTVTEDAREFAIKP
                     MNCPGHIQIFKHGLRSYRELPYRVAEFGIVHRYEPSGAMHGLMRVRGFTQDDAHIFCT
                     HEQVAEECLKINDLILSIYRDFGFEEVVVKLSTRPEKRVGADALWDQAEADMSQVLDV
                     IARQSGGKIKTGINPGEGAFYGPKFEYVLRDAIGRDWQCGTTQVDFHLPARFAAFYID
                     ADGEKKTPVMIHRAIFGSMERFAGILLENTAGHLPLWLAPVQAKVLTIVSEADAYAGE
                     VLAALGRAGLRAEADLRNEKINYKVREHSLAKVPVLLVVGKREAEERTVSLRRLGTPQ
                     QEVLPLEAALGRLAQQCVPPDLEPGRA"
     gene            complement(7027..>7339)
                     /locus_tag="VFR00_03405"
     CDS             complement(7027..>7339)
                     /locus_tag="VFR00_03405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0158331.1"
                     /translation="RYVTAENLPSLFGYRETYRTSIRPALGLKEPPTALAGTVFAGSF
                     VDRMASFYARVRRAVFYSLTRFEVEVIDRYQPERRYVSTFELRGWEWKLTALAVLGAG
                     Y"
BASE COUNT         1301 a         2440 c         2364 g         1234 t
ORIGIN      
        1 gatttgagct tccttgaggc gcgcatcgag aacggtgcag agccgttgcc cggtgatcag
       61 ccccgagccc gagaaattga agtcgagaaa gcccagcacc ttgcggcgcg tcagctcacg
      121 cgcgaactgc tcaagcgcct cgagctcgcc agcgacgtag cagccgccaa cgacggcgcc
      181 gatcgaggtg ccggcgacaa tgtccgggaa gatgccggcc ttctgcagcg cccgcagcac
      241 gccgatgtgc gcccagccgc gcgccgaacc gccacccagc gccaaaccga tcttacaatc
      301 gctcatgcga gtccccatgc ggcgcttgtc atagatcgaa tttttaaaat cttgccccgc
      361 gcgtcaacca tagcggtgag gaattaagga gccctaggcg ctcagggtta atgctcagct
      421 aggcagtgtg ccagcgacga tagtgacgcc gaggcgcggc cagggacagc aaacaggcaa
      481 aaaagcctta accacgcgtc agcccgatcg cctcggcggt gcccaccggc agcgtcaatt
      541 tgcggtaaaa acacgagcgc gcgcccgtgt gacaggcgac gccggcccct tccaccgtga
      601 cgcgcagcca cagcacatcc tggtcgcagt ccgtgcgcac ctcgctgacc cgtagcaggt
      661 tgccgctctc ctcccccttc ttccaaagcc tgcggcgcga gcggctccag aaatgcgcaa
      721 aaccgctggt cagcgtcagc tgcaaggctt ccgcgttcat ggcggcaaac atcaagacct
      781 cgcccgtggc ggcatcgctg acgatcgccc caatcaggcc gtcggcatcg aatttgggct
      841 ggaatgacag accctgctcg acctcgtcgg ggctgccacg cggtgcaaag gcgggtgatg
      901 ccagaaaagc catggcgcat ctccaaaaaa agtcggcagc ggtgggcttc agggccgcgc
      961 cgcgtgcacc atctgcagga agcggtcgcg gtagctggcg tcctgctcca aagcgcccgt
     1021 gaagcgcgtg gtgatggttt tgacgccggg ttggcgtact ccgcgcgtgg acatgcactg
     1081 atgctcggcc tcgatcagca ccgccacccc gcgcgggcgc agcgcggcct cgatagcgtc
     1141 ggcgatctgc gccgtcatgg tctcctgggt ctgcagccgc ttggcgaaga tctcaaccac
     1201 gcgtgcgatc ttcgagatgc cgacgatgcg accgttaggc agataggcga cgtgggcgac
     1261 gccgaggaac ggcgccaggt ggtgttcgca atggctttcg acgcggatat cgcgcagcat
     1321 cacgagatcg tcgtaaccgc ccgcctcgtc gaattggcgc gccagcacat ccgtggggtc
     1381 gagccgatag ccggagaaat actcctcgaa ggcttccgtc acgcgccgtg gcgtgtcctt
     1441 cagtccttcg cgctgggggt catcgccggc ccaggcaatc aaggtgcgca ccgcggcctc
     1501 ggcctcgctg cgcgtggggc gctggcgcga ttgatccagc ctgacgctcg ggcgtttctt
     1561 tttggcgacg atatcgtcca ttgatcttgc cgctgtaaaa ctcaggtcga gggatggcgc
     1621 cgcggcgggg aggcacacgc gcctgaacga cggggacgcc gttgaaatct ccaagcgcgc
     1681 acgcgatatt tccccatagc tgccgcccgg tcccggtttg cgacagcaaa caacggttgc
     1741 ggtgggatat agtaccttag actggttggg gcaagaatcc cctgaacttc atacctggcg
     1801 aggcgatcgg gcctgatgac gcagctgagt gaaatctaca acgcacgcat ccttgagctg
     1861 gccgccaaca tgcccaagcc cgatcggctc gccgacgcgc atggcagcgc gaccgctcac
     1921 tccaagctgt gcggctcgac cgtcagcgtg gacctgcagc tgaccgatga acgcgtgtcc
     1981 gcctatgggc agaccgtgaa ggcctgtctg ctcggccaag ccgcggcctc cgtgatgggg
     2041 cacaacatca tcggcagcac ggccgaagag ctgcgcgacg tcgccctgaa aatgcgcaag
     2101 atgctgaaag agggcgggtc ggcaccgcag gggcgctggg ccgatcttgc cgtgctcgaa
     2161 cccgtcaagg actacaaggc acgccacgcc tcgacgctgc tcgtgttcga cgcggtcgag
     2221 gcggcgctcg ccaaggccga ggcgcgccag gagccagtgc agcatgccgg tgtctagccc
     2281 ccggcgcgcg cgccccaccc gggccgccgg ggcggccaag ttcgccgccg cgcttcccat
     2341 tcatctttac cgctggacct tgaagccgct gctcggccac gagtgccggc atttgcccac
     2401 ttgctcggaa tatgcgctcg atgccatcgc ccgcaacggc gcctggcgcg gcggctggct
     2461 cgccttgtcg cgcctgtgcc ggtgccatcc ctggggcacg cacggcttcg acccggcgcc
     2521 ggatctcgcc ggcaggcggc atcgcttggc cccgtggcgc tacggggtgt ggcgcgcgcc
     2581 ccgcccgcag cgctagggcc gccggggctc accctcgctc gttcgcaagg cttgcgctgg
     2641 cgtgccggct caattggcgc ccaggcgccg cagccgctcg tcggcgtcgc cggcgcccaa
     2701 ctggcgcgcg cgttcatacc actgccgcgc cagcttgctg tcgggcttga tgccgcgcac
     2761 gttgcggcgg ccgagctcgg tcgcatcaaa ggtggcggcc agcgccatgg cgccttccgc
     2821 caggcccgca tcggcggccc gctcgaagaa gaggcgcgcg gcggaaacgt tgccttcctc
     2881 ccactgctcg ttgcccttgg cgaggaggcg catggcgcgt tcgcgttccg gcgactggga
     2941 gcgcggcacg atgggcggaa ccgccgcggt cgtctcctgg cgcggcgggg cgagttcgac
     3001 gggcacgccg gcccgcagca tcgaggcgcc gccggggggg ccggcgctgc gctcgcggac
     3061 cgggttggcg gcctgcgcca cggtcagcgc ggttttgctt tccaccagca ccgcgccgtc
     3121 caccgcaacc aacaagatcg tcacctccga tcggcccgcg gcggcgaccg gtagcgagat
     3181 cttgaggtca ggcagggccg agatcggcac cgcccaggat cccgcgccga tcacatagcc
     3241 ctcggagagc gcggccatgg gcggcaagcc gcgcaggcgc acgaagctgt tgcgcggaat
     3301 ggcctcggcg ggctcgatgg cgatcgggaa gggcacctgg cttgccgcct cggcgacgac
     3361 ggccggaggt accctgatga taatttggcg cgagctctga ccgaaggcgg gagcgccgag
     3421 gatcaggctt gacagcagta gcccggtggc aaacgcacgc cctagctgcg gggggcatcc
     3481 ccgatcacgc ctcggattgt tcacgtcgcg tcgccctccc ctggccacgc tcccctgtgg
     3541 agatgtatgc ctcgctaacg ccgctgggtc ttgctccagt ccatgaactc gcggaacagg
     3601 cgctcctgct cggcctgatc attgggtgca gctttggccg cctgcgcccg gataatggac
     3661 ggatcgatgg ccggtggtgc aaccgaggcg gtcgaggccg gcctttctcc gttcttggcg
     3721 atccactctt ccgcggctgg aaagcgtttc cagccccgca ggttcgaaag caggttggcc
     3781 tcgcgccact tgctatggcg ggcgggcttc tgaaattcgc cgaacttgga aaagaaagcg
     3841 tcgatgaact gcgccacgcg gcggtagcga tccgtgtcgc gcgcccaatt ataggaggcg
     3901 aggaccgcac cgatcgcgac ggtgtcgacc gagctgcctt tggcgacgag gttgggatag
     3961 tcctcggagg tcagcttggc agggaaatag tcctgctcca gaacttctgt atacggcacc
     4021 ggcagcaagg tcatgccggg ctccagtttg aacttgccga aggcgcccgt cggcttgccg
     4081 gcgatcagga tggtggccgc gatctcgccc gacttcacca tctgatagcc atcgccctgt
     4141 ccgacgttga cctcctcggc cttgatattg agcaactcga aaatcagccg ggtggaaaac
     4201 tgcgtgccgc tgccgacgtc gctgaagttc accttcttgc cgttgagatc ctggatcgac
     4261 ttgatgcccg ggccggcgag cacgtgcatc tcctcgttgt agagtttggc gatgtaggcc
     4321 agacggttgt cgatggtcga tccgaattcg ccgctcttct tcagatagct catgatgttg
     4381 gattgcgtga tgcacaggtc gacgccgcgc aagtgcagca cgtcgaccac gttctggtat
     4441 ccgcccttgc cgatcacggg caggacgcgc agctcgttgc cgtcgtccaa caccgccgac
     4501 atgtcgtagg cgagataaag ataagtgccg ttggggttac ccgagacgat tgtcaccgtg
     4561 ttctggttga gcttctcggt cagcgcctga tgttgcgccg gggttgccac cgccggtcgc
     4621 ttggccggtg gcggcgattt tacttgcggt agctgctggg cataaccgtc gaccgggacc
     4681 gacagcagcg cggttactgc cagcgccaaa aggccaagga ttgcgtgcac gtcacctctc
     4741 ccctcgcagc caaaacgcgt gaccaaactc ggcatgcgcg ccctattgcg gtgcgtacct
     4801 aagctgccac gaccccccaa atgggacgaa attaacacat ggaagacgcg tggcaacgca
     4861 agcccgcgtg ctccaaatgc cgaattgtac gcacaggttg ggcacaagtg tggctttccc
     4921 atgcgccctt gacaggcgcc gcagccgggg ggcaaaaggc gccccgcggg ggaggtccgc
     4981 tcccctcccc ggcaaaagcc accttacctg cgcggtatgc agtgagtgag aggaaagggc
     5041 gatgagcgaa atcaccttga catttccgga cggggctgcc cgcccctacc cccgggggat
     5101 caccggcaag agcctggccg agcgcatctc caagacgctt gccaagcgcg ccgtggccat
     5161 gctggtcgac ggccgcctag ccgatctcgc cgaccccttg ctggccgacg ccaaggtgaa
     5221 actcatcacc cgctccgatc ccgaggccct cgagctcatc cggcacgatg cggcccacgt
     5281 catggccgag gcggtccagt cgctgtggcc tggcacccag gtgaccatcg ggcccgtcat
     5341 cgacaacggc tttttttatg atttcgccaa ggcggagccc ttccaccccg acgatctcgg
     5401 caaaatcgaa gccgcgatgc gccagatcat tgcccgcgac gcccccttca gcaaagaatt
     5461 ctggagccgc gaggaggcca agtcttactt tcgcaagaag ggcgagctgt tcaaggtcga
     5521 gctgatcgat gccatccccg cgggggagga catcaagatc tacaagcaag gtgactggct
     5581 cgacctgtgt cgcggcccgc acatgacctc gaccggccag gtcggcagcg ccttcaagct
     5641 cacccatttc gccggctcct actggcgcgg agacgcccag ggccagcggc tccagcgcgt
     5701 ctacggtacg gcctgggcca gcgatcagga gctcgccacc tacctggcgc agctggcgga
     5761 agccgaaaaa cgcgatcacc gcaagctcgg gcgggagatg gatctgttcc acttccagga
     5821 ggaggggccc ggcgtcgtct tttggcaccc caagggctgg cagctcttcc agagcctgat
     5881 caactacatg cgccggcggc aaaatgccgt cggctatcgc gaggtgaacg ccccccaact
     5941 gctcgacaag tccctgtggg agacctctgg gcattggcag acctttcgcg aaaacatgtt
     6001 caccacggtg acggaggatg cgcgcgagtt cgccatcaag ccgatgaact gccccggcca
     6061 catccagatc ttcaagcacg gtttgcgctc ttaccgggag ctgccctacc gcgtggccga
     6121 gttcggcatc gtgcaccgct acgagccgtc cggtgccatg cacggactga tgcgcgtgcg
     6181 cggctttacc caagacgacg cccacatctt ttgtacgcac gagcaggtgg ccgaagagtg
     6241 cctcaagatc aacgatctga tcctgtcgat ctatcgtgac ttcggcttcg aagaggtggt
     6301 cgtcaaactg tcgacccgcc ccgagaagcg cgtgggtgcc gatgcgctct gggaccaagc
     6361 ggaagcggac atgtcccaag tgctggatgt gatcgcccgc caatccggcg gcaagatcaa
     6421 aaccggtatc aatcccggcg agggggcctt ttatggcccg aaattcgaat acgtgctgcg
     6481 cgatgccatc ggccgcgatt ggcaatgcgg caccacccag gtcgacttcc acctgccggc
     6541 ccggttcgcg gccttttaca tcgacgcgga cggcgaaaag aaaaccccgg tcatgatcca
     6601 ccgggccatc ttcggttcca tggagcgctt tgccggcatc ctcttggaga acacggctgg
     6661 ccacctgccg ctgtggctgg cgcccgtgca agcgaaagtc ttgaccatcg tctcggaggc
     6721 ggacgcctac gccggcgagg tcttggccgc cctcggccgg gcgggcctgc gcgcggaagc
     6781 cgatctgcgc aacgagaaga tcaactataa ggtgcgcgag cactcccttg ccaaggtgcc
     6841 cgtgctgctc gtggtgggca agcgcgaggc cgaggaacgc acggtatccc tgcgccggct
     6901 cggcacgccc cagcaagagg tgctcccctt ggaggccgcg cttggccggc tggcgcagca
     6961 atgcgtgcca cccgatctcg agcctggccg ggcttaggcc ccgggctggg gctgaccctt
     7021 ggcgcactaa tagccggcac ccaacacggc aagcgccgtg agcttccact cccagcccct
     7081 gagctcgaag gttgagacgt agcgccgttc cggttgatag cggtcgatga cttcgacctc
     7141 gaagcgcgtc agcgaataga acacggcgcg ccggacgcgc gcgtaaaaac tggccatgcg
     7201 atcgacgaag ctgcccgcaa aaacggtgcc ggccagcgcg gtcggcggct ctttcaagcc
     7261 cagcgcaggg cgaatgctgg tgcggtaggt ctcgcggtag ccaaacaacg agggcaggtt
     7321 ctcggccgtc acatagcgg
//
LOCUS       DASTMT010000153         1937 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_739673, whole genome shotgun sequence.
ACCESSION   DASTMT010000153 DASTMT010000000
VERSION     DASTMT010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 1937)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1937)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1937
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_739673"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(169..489)
                     /gene="soxZ"
                     /locus_tag="VFR00_03410"
     CDS             complement(169..489)
                     /gene="soxZ"
                     /locus_tag="VFR00_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011925448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate oxidation carrier complex protein
                     SoxZ"
                     /protein_id="HEU0158332.1"
                     /translation="MARALIKVSPNPKRGEIIEIKTLISHEMETGFRHTNTGAAIARD
                     IITTFVVTYNGEEIFSAQLFPAIAANPFITFHTIAKESGTLEFRWTGDNGYVASEQVK
                     IAVA"
     gene            complement(489..989)
                     /locus_tag="VFR00_03415"
     CDS             complement(489..989)
                     /locus_tag="VFR00_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008967224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxY-related AACIE arm protein"
                     /protein_id="HEU0158333.1"
                     /translation="MAETSKHLGAAAAGTRRQLLQFAGSAALGGMLPSLAVRPAAATP
                     ASMQSAIAAVIGDAKLNSGRVKLEIPPLVENGNTVACAVAVESPMSAADYVKAIHVFN
                     EKNPQPNVISVKLGPSAGRAAIATRIRLTDTQTVTAIAEMSDGSFWSQSVEVVITLGA
                     CLEDPL"
     gene            complement(998..1426)
                     /gene="soxX"
                     /locus_tag="VFR00_03420"
     CDS             complement(998..1426)
                     /gene="soxX"
                     /locus_tag="VFR00_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007594053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur oxidation c-type cytochrome SoxX"
                     /protein_id="HEU0158334.1"
                     /translation="MLWSACPGGAAWTQAAPLVSFSVSGDGIERSLTGVPGDSARGRA
                     IVTNRQVGLCLLCHSGPFPEERLQGTLAPDLKGAGSRWSEAELRLRIVDAAVINPKTI
                     MPPYYRLEGLERVAPAFRGKPILTAAQIEDVVAFLATLKE"
     gene            complement(1480..>1937)
                     /locus_tag="VFR00_03425"
     CDS             complement(1480..>1937)
                     /locus_tag="VFR00_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018457155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FCSD flavin-binding domain-containing protein"
                     /protein_id="HEU0158335.1"
                     /translation="VANVIPPQKAGRAAVLAGAVDQTGWCPIDPVSFESKTSPNVHVV
                     GDAIIAGGMPKSAFAANAQAKVCAAAVVDLLAGRTPVQPKLINTCYSLVAPDYGISVA
                     GVYAPVNGVLTDVPGAGGLSPLEAPRTERAAEAVFAEGWFNTITAEVFG"
BASE COUNT          320 a          659 c          641 g          317 t
ORIGIN      
        1 tcatcgcttc atagccggag cggcgctcat tgagcggcat gtcggcggcg aggccgaccg
       61 acgccagcgt catggcggcc gcagcaacgc caagccgacg ggcgggcccc gccagccgcc
      121 gcaccgcgcc ggggcctggg catgctgatc gcaccggcgc tgcccgcgtc atgccaccgc
      181 gatcttcacc tgctcgctgg cgacataacc gttgtcaccg gtccagcgga actcgagcgt
      241 gccactctcc ttggcgatcg tgtggaaggt gatgaaggga ttggcggcaa ttgccggaaa
      301 cagctgggca gagaaaatct cctcgccgtt gtaggtcacc acgaacgtgg tgatgatatc
      361 gcgggcgatg gccgcgcccg tattggtgtg ccgaaagccc gtctccatct catgcgaaat
      421 cagggtcttg atctcgatga tctcgccccg tttgggattg ggggatactt tgatcaatgc
      481 gcgagccatc agagggggtc ctccagacag gcgccgagcg tgatcacgac ctcgacgctc
      541 tgcgaccaaa aagagccatc ggacatctcg gcgatcgccg tcaccgtctg cgtgtcggtg
      601 aggcggatgc gggtggcgat ggccgcccgc ccggcgctcg gcccgagctt gacgctgatc
      661 acgttcggct gcgggttctt ttcgttgaag acgtggatgg ccttgacgta gtcggcggcg
      721 ctcatcgggc tctcgaccgc cacggcgcac gccaccgtgt tgccattctc aaccagcggc
      781 gggatctcga gcttgacccg gccgctgttg agcttcgcat caccgatcac cgcggcgatg
      841 gccgactgca tgcttgccgg cgtggccgcg gcggggcgca cggcgaggct cggcagcatg
      901 ccgcccaggg cagcgctgcc ggcgaactgc agcagctggc gccgtgtgcc ggccgctgcc
      961 gcccccagat gcttgctcgt ctcggccata gcccggctca ctccttcaac gtcgccagaa
     1021 acgccaccac atcctcgatc tgcgccgccg tcaatatcgg cttgccccgg aaggcgggtg
     1081 cgacccgctc aagaccctcg aggcgatagt aaggcggcat gatcgtcttg gggttaatga
     1141 cggccgcatc gacaatacgc aggcgcagct ccgcctctga ccaacgcgag ccggcgcctt
     1201 tgagatccgg tgccagcgta ccctgcagcc gctcctcggg aaatggcccg gaatggcaaa
     1261 gcaggcagag cccaacctgg cggttggtga cgatcgcacg cccgcgcgcg ctatcgccgg
     1321 gcacacccgt cagcgagcgc tcgatgccat cgccgctgac gctgaagctc acgagcggtg
     1381 ccgcctgggt ccaggcggcg ccgcctggac aagcactcca cagcaccgcc acggcgatgg
     1441 cgcccagcac cgttgtcttc tgtctgtcgc gcccgcccct caaccgaaca cctcggcggt
     1501 gatggtgttg aaccagccct cggcaaacac cgcctcggca gcgcgctcgg tccgcggcgc
     1561 ctcgagcggg ctgaggccgc cggcgccggg cacatcggtc aagacgccgt tgacgggtgc
     1621 atagacgccg gcgacgctaa tgccgtaatc gggcgccacc aggctgtagc aggtgttgat
     1681 caatttcggt tgcacgggcg tgcggcccgc cagcaagtcg acgacggcgg cagcacagac
     1741 cttggcctgc gcattggcgg caaaggccga cttgggcatg ccgccggcaa tgatggcatc
     1801 gccaacgacg tgcacgttag ggctcgtttt ggattcgaag ctgacggggt cgatcgggca
     1861 ccaacccgtc tgatcgacgg cgcccgccag cacggcggcc cggcccgcct tttgtggcgg
     1921 aatgacgttg gcaacgg
//
LOCUS       DASTMT010000154         5169 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_1724293, whole genome shotgun sequence.
ACCESSION   DASTMT010000154 DASTMT010000000
VERSION     DASTMT010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 5169)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5169)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5169
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1724293"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..244)
                     /locus_tag="VFR00_03430"
     CDS             complement(<1..244)
                     /locus_tag="VFR00_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010911747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEU0158336.1"
                     /translation="MDKRKLGNSGLAIAPLIFGSNVFGWTADEATSFKLLDAFLAAGF
                     DCIDTADVYSKWVPGHRGGESEAIVGNWLKARGNRDK"
     gene            complement(365..4075)
                     /locus_tag="VFR00_03435"
     CDS             complement(365..4075)
                     /locus_tag="VFR00_03435"
                     /inference="COORDINATES: protein motif:HMM:NF024893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HEU0158337.1"
                     /translation="MTRTGGQGARRVAETTARPTTRPSTRRPPAAVRPWHGAFVLLGK
                     AAGFTTLVLALGAGLFYARLLNGPVPLSFLVQPLERAIAEELAGLGVAIDNVAARLNE
                     RHQLEFELSNVRIADSNNAPLAVAPSATFSISRRALLQGRIAPESVDLISPRLLLYLG
                     EDGKLSLQFATATEAAEGDPHKAGGGEVPGKAGALASAIGANDGSLGRIDLVKMLSEA
                     SARARRREYATAYLREVGLRSATLIVDNGSRKSIWRVPELDVDLDHRRSRSSIAGRAK
                     VESLAGPWDLNFRTIEVEKAGTLQLAVSVQNLVPRGLARTLPHLAALEALDLPVWGEA
                     YLDLSSDGDILGGRIGIDAAPGTVVLPWLAENPFNIDGGHLEISYQSEARRFEIGSSV
                     LVWGDSRVQFGGSLVHEARAPEGPAWAFALKSQGGWIGAELPAHERLAIDDWSAQGFL
                     APERGRVVLSQFSLKAGGAEVTVEGDVAGMGSAASARLDGKIGPMPAKTFKALWPAAL
                     APQTRAWVGQHLVRGQLQGGAFKLTSSDQPQGADRTLPSSPADRVSLTLVGSELALAL
                     SDAWPPLELPRALARLDGHTFELSAPEGAFVAPDGRRIGVKGSLNIDMQEPLPRTGHI
                     TAKLQGPLSLAMEMVAQEPFQVFKDTWIDPSAADGKLEAHIALALPLQHTVTPADLKI
                     DGKARISEGRIPQIFPPYDLHGANIMLELTGTAVDVRGDMLIKGVPVKASWQHVFGVP
                     GGKQPPLTITANLDANDRTQLGLDINDLVLGEVATEITVSRDANNEQRVHLRADLANA
                     ELVLDSIAWKKPRGRASTFQCDIVRNGASHPIELMNVQLVGDNVAIEGWMGIGPDHHV
                     REYRFPNFSINVITSLQAYGKMRGDGVWEVTAKGPRYDGKDLFQSFFDVASIGERDGK
                     VRPGVDLRAEIDTVVGYNDSALRNVKVWLQKRNNKLTALDARGVLEGNKAFAAVLRPE
                     AGQPRLLRAEAIDAGQTFKLVGFYSSAVGGHMNLEVNLDGSGVAQRTGTLWAKNFVLL
                     GDAIGGDFLVQGPDGAMRPMGSAEKRGIVREQFQFESLRVPFSVGHGQFLLNEARLEG
                     PVLSANMRGKIDFRSQNVNVGGTFTPLAGLNTMFRGLPLFGPLLTGPRGDGVFAMTFA
                     IQGSMGAPQIIVNPLSVITPGVTREIMQMAPEQQRIVPRDRPAARSESGSRPTTAPAA
                     PTSSNGFEARVQPEIGGGWSADSEPPAPAKKR"
     gene            complement(4258..>5169)
                     /locus_tag="VFR00_03440"
     CDS             complement(4258..>5169)
                     /locus_tag="VFR00_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009028271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsH"
                     /protein_id="HEU0158338.1"
                     /translation="KVPLAPDVDPKVIARGTPGFSGADLANLVNEAALLAARRNKRLV
                     TQAEFEDSKDKVMMGAERRSMIMTEEEKLATAYHEGGHALVNLLVPGNDPLHKVTIIP
                     RGRALGVTMSLPERDKLSYSKQWCEARIATMFGGRVAEQLVYGEDHLNTGASSDIMQA
                     TELARRMVTEWGMSDRLGPLRYNENQQEVFLGHAITQRQNMSEDTARLIDEEIRRIVT
                     EGERKAREVLGGNRDKLEAITQALMEYETISGEEVNALMRGEKIVRANDDEESKGPAG
                     PAVPAAGKMRPSRPEPGPGGLEPQPQT"
BASE COUNT          817 a         1738 c         1716 g          898 t
ORIGIN      
        1 ccttatcccg gttgccgcgc gccttcagcc agttgccgac aatcgcttcc gattcaccac
       61 cgcggtggcc gggcacccac ttggagtaaa cgtcggccgt atcgatgcaa tcgaagccgg
      121 cggcgaggaa ggcgtcgagc agcttgaagg aggtggcctc gtccgccgtc cagccgaaca
      181 cgttgctgcc gaagatcaag ggcgcgatcg ccagtcccga atttccaagt ttacgcttgt
      241 ccatgcgtct ccccgtgcga ggcggtgaag gccgagccat gccgatgcca ggcagcggca
      301 gcggtagatt tgcaactatg gcacgcgccc tcccgcagca acagctcgcg ccttgtcgcg
      361 gttgctaacg cttcttggcg ggcgcggggg gttcgctatc ggccgaccag ccgccgccga
      421 tctcgggctg cacgcgtgcc tcgaagccat tgctcgaggt cggcgctgct ggcgccgtcg
      481 tggggcgcga gccgctctcg ctgcgcgcgg ccggccggtc gcgcggcacg atgcgttgct
      541 gctccggcgc catctgcatg atctcacgcg tcacgccggg cgtgatcacg gacaacggat
      601 tgacgatgat ctgcggcgcg cccatcgagc cctgaatggc aaaggtcatg gcgaacacgc
      661 catcgccgcg cggcccggtc agcaggggac cgaacagcgg caggccacgg aacatggtat
      721 tgagcccggc caacggcgtg aaggtgccgc cgacattgac attttgcgag cggaaatcga
      781 tcttgccgcg catgttggcg ctgagcactg ggccctcgag ccgcgcctcg ttgagcagga
      841 actgaccgtg ccccaccgag aagggcacgc gcaagctctc gaactggaat tgctcgcgca
      901 cgatgccgcg cttctcggcc gagcccatcg gccgcatggc gccgtccggg ccttgcacca
      961 ggaaatcgcc gccgatggca tcgcccagca agacgaagtt cttggcccac agcgtcccgg
     1021 tgcgttgcgc cacgcccgag ccgtcgagat tgacctccag gttcatgtgg ccaccgacgg
     1081 cactgctgta aaagccgacc agcttgaagg tctgcccggc atcgatggcc tcggcgcgca
     1141 gcaatcgcgg ctgtccggct tccggccgga gcacggcggc gaaggcctta ttgccttcga
     1201 gcacgccgcg cgcgtcgagc gccgtcagct tgttgttgcg tttctgcagc cagaccttga
     1261 cgttgcgcag cgcgctgtcg ttgtagccga ccacggtgtc gatttcggcg cgcaggtcca
     1321 cgcccggccg caccttgccg tcgcgctcgc cgatcgaggc cacatcgaag aaggattgaa
     1381 acagatcctt gccgtcgtag cgcgggccct tggcggtcac ttcccacacg ccatccccgc
     1441 gcatcttgcc gtaggcctgc agcgaggtga tgacgttgat ggagaaattg gggaaacgat
     1501 attcgcgcac gtgatgatcg gggccgatgc ccatccagcc ttcgatggcg acgttgtcgc
     1561 cgacgagctg gacattcatc agctcgatgg ggtggctggc gccgttgcgc acgatatcgc
     1621 attggaaggt gctggcgcgc ccccttggtt tcttccaggc gatgctgtcg agcacgagtt
     1681 cggcgttggc aagatcggcg cgcagatgca cgcgctgctc gttattggca tcgcgcgaga
     1741 ccgtgatttc ggtggccact tcgcccagca ccaaatcatt gatgtcgagg ccgagctgcg
     1801 tgcgatcgtt ggcatccaga ttggccgtga tggtgagcgg tggctgtttg cccccgggca
     1861 caccgaacac gtgctgccag ctggccttga ccggcacgcc cttgatcagc atgtcgccgc
     1921 gcacgtcgac cgccgtgccc gtcagctcca acatgatatt ggcgccgtgc aggtcgtagg
     1981 gcggaaagat ttgcggtatg cgcccctcgc tgatgcgggc cttgccgtcg attttcaggt
     2041 cggccggcgt cacggtgtgc tgcaatggca gcgccagggc aatgtgggcc tcgagcttgc
     2101 catcggccgc cgacggatcg atccaggtat ccttgaagac ctggaacggc tcctgtgcca
     2161 ccatctccat ggcgagcgac aacggtccct gcagcttggc cgtgatgtgg ccggtgcgcg
     2221 gcagcggctc ttgcatgtcg atgttcaggc tgcccttgac gccgatgcgt cgcccgtcgg
     2281 gggcgacgaa ggcaccctcg ggcgccgaga gttcgaaggt gtgaccgtcg aggcgcgcca
     2341 acgcccgcgg cagctccagc ggcggccagg cgtcgctgag cgcaagcgcc agctcgctcc
     2401 caaccaacgt gagggagacg cgatcggctg ggctgctggg taaggtgcgg tccgcgccct
     2461 ggggttgatc gctcgaggtc agtttgaagg ccccaccctg gagttggccg cgcacgaggt
     2521 gctgcccgac ccaggcgcgg gtctgcggcg ccagggctgc cggccagagc gccttgaagg
     2581 ttttggcggg catgggcccg atcttgccgt cgagccgcgc gctcgcggcg ctgcccatgc
     2641 cggcgacatc gccctcgacg gtcacttcgg caccgccggc cttgagcgag aactggctca
     2701 acaccacgcg gccgcgctcg ggcgccaaaa agccctgcgc cgaccaatcg tcgatggcaa
     2761 ggcgctcgtg cgcgggcagc tccgcgccaa tccatccacc ctgtgatttc agcgcgaagg
     2821 cccaggcggg accctccggc gcccgggctt cgtgcaccag gctgccgcca aactgcaccc
     2881 ggctatcacc ccataccagg accgaggagc cgatctcgaa gcgccgcgcc tcgctttgat
     2941 aggagatttc caggtgtccc ccatcgatgt tgaaggggtt ctccgccaac cacggcagca
     3001 ccaccgtgcc cggcgccgcg tcgataccga tgcggccgcc gagaatgtcg ccgtcgctcg
     3061 agaggtcgag gtaggcttcc ccccagacgg gcaaatccag cgcctcgagc gctgccaagt
     3121 gggggagcgt gcgcgccagg ccgcgcggca cgaggttctg caccgagacg gcaagctgca
     3181 aggtgccggc cttttccacc tcgatggtgc ggaagttaag atcccagggg ccggccagcg
     3241 actccacctt ggcacgtccc gcgatcgaac tgcggctgcg ccggtggtcg agatcgacgt
     3301 cgagctcggg cacgcgccag atgctcttgc ggctcccatt gtcgacgatc agcgtggccg
     3361 agcgcagtcc gacttcgcgc aaataggcgg tggcatattc gcggcgacgc gctcgcgcgg
     3421 aggcctccga gagcatcttg acgagatcga tgcggccaag gctgccgtcg ttggcgccga
     3481 tggcggaggc gagcgccccg gccttaccgg ggacctcgcc gcccccagcc ttgtgcggat
     3541 ccccctccgc ggcctcggtg gccgtggcga actggagcga gagcttgccg tcttcgccga
     3601 ggtaaagcag aagtcgcggc gagatcaggt cgacgctttc cggtgcgatg cggccctgca
     3661 gcagcgcccg acgactaatg gaaaaggttg ccgagggcgc cacggcgagc ggggcattgt
     3721 tgctgtcggc gatgcgcacg ttgctgagtt cgaactcgag ctgatggcgc tcgttgagcc
     3781 gcgcggcgac gttgtcgatg gcaacaccga gccctgcgag ctcttcggcg atggcgcgct
     3841 cgagcggctg caccaggaag gaaagcggca ccgggccgtt gaggagccgc gcgtagaaga
     3901 gacccgcccc gagcgccagc acgagtgtgg tgaagcccgc cgccttgccc aacagcacaa
     3961 aagcgccgtg ccagggccgc accgccgccg gcggtcgcct ggtcgagggg cgtgtggtgg
     4021 gtcgcgcggt ggtttcggcc acccgccttg ccccttgccc gcccgtcctc gtcacgacgc
     4081 tccctcaatc atgtggcgcc gagctgtccc cttgagagcg ataaagcaaa gaacccgccg
     4141 cggtcagcgg cgtgtgataa cgcaacccca cgcttgctac caacaaagcg aggcaaaaag
     4201 gacgaaagga aggcgcctca acgcgcacgt cacgcttttg gcttggcgcc agccgtttta
     4261 cgtttgcggc tgcggctcaa gcccacccgg acccggttcc ggccgcgacg ggcgcatctt
     4321 gccggccgcc ggcaccgccg gtcctgccgg ccctttcgac tcctcgtcgt cgtttgcccg
     4381 gacaatcttc tcgccgcgca tcaaagcgtt cacctcctcg ccgctgatgg tctcatactc
     4441 catcagcgcc tgggtgatgg cctccagctt gtcgcgattg ccacccagca cctcgcgcgc
     4501 cttgcgctcg ccttcggtga cgatgcggcg gatctcttcg tcgatcagcc tggcggtatc
     4561 ctcggacatg ttctggcgtt gcgtgatggc gtggccgaga aacacctcct gctggttctc
     4621 gttgtaacgc aagggaccga gcctgtcgct catgccccat tccgtcacca tgcggcgggc
     4681 gagctcggtt gcctgcatga tatcgctgga ggcgccggtg ttgaggtggt cttccccgta
     4741 cacgagctgt tcggcgacgc ggccaccgaa catggtggcg atccgcgcct cgcaccactg
     4801 cttggaatag gagagcttgt cgcgctcggg caggctcatg gtgacgccga gcgcgcgccc
     4861 gcgcggaatg atcgtgacct tgtgcagcgg gtcgttgccc ggcacgagca ggttgacgag
     4921 agcatgcccc ccttcatggt aggcggtggc gagcttctcc tcctcggtca tgatcatgga
     4981 acgccgttcc gcgcccatca tcaccttgtc tttgctgtcc tcgaactcgg cctgcgtcac
     5041 cagccgcttg ttgcgtcggg cagccagcag cgccgcctcg ttcaccaggt tggcaaggtc
     5101 ggcgccggaa aaaccgggcg tgccgcgggc gatcaccttg ggatcgacgt caggcgccag
     5161 ggggacctt
//
LOCUS       DASTMT010000155         2006 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_86279, whole genome shotgun sequence.
ACCESSION   DASTMT010000155 DASTMT010000000
VERSION     DASTMT010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2006
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86279"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..942
                     /locus_tag="VFR00_03445"
     CDS             <1..942
                     /locus_tag="VFR00_03445"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEU0158339.1"
                     /translation="HHYVPAQSNQQKFWFEPWAKKLEQDSGGRLKVDIYPSMQLGGRQ
                     PQLFDQARDGVVDIVWTVAGSTPGRFPRLEVFELPFLSHAIGEKTAPAVWEFYETFAK
                     DELKDVKPLAVWGFGKGILFTKDVVIKTPADAKGLKLRASNRQTNDAFALLGAQPQSI
                     PPPGVPEALAKGVVDGVVFPYDAVIPFKLDELTNRVSEFGGDRTFYNSVLLFVMNKAK
                     YDGLPDDLKKVIDANSGAALSRDLGRKWDEWDQIGRAAVKKRNTPIHVIDGADLAAWK
                     TATEPIFAKWIEERDKAGDKGADLVRAAQALVAKYSQ"
     gene            1029..1520
                     /locus_tag="VFR00_03450"
     CDS             1029..1520
                     /locus_tag="VFR00_03450"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HEU0158340.1"
                     /translation="MLGGVLLIGIMLISSLSVLGRGLSLLVGARISGIPGDVEIVQLG
                     CAVAIFAFLPICQLKRANVLVGALTKHLPVRARASFDLAANALFLLLTFLLAFQLGVG
                     TREKFANHDTTMVLRIPEGWAYAVALVFAWLLVLTTAYTAARSAAEIRRNRAIGPSPA
                     GER"
     gene            1530..>2006
                     /locus_tag="VFR00_03455"
     CDS             1530..>2006
                     /locus_tag="VFR00_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009451633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEU0158341.1"
                     /translation="MSNFAAGLSSFVLLLMLLALRVPIGVAMLAVGVGGFIWIQGLAP
                     LLSFLKTTPFETFAGYSFSVIPLFLLMGNVASGSGLSARLFRGANALLGHHRGGMAMA
                     AVGACAAFGTICGSSLATAATMGQVALPEMRKYGYSGALSTGTLAAGGTLGILVPPS"
BASE COUNT          294 a          678 c          659 g          375 t
ORIGIN      
        1 caccactatg ttcccgccca atccaatcag cagaagttct ggttcgagcc ttgggcgaaa
       61 aagctcgaac aggatagcgg tggccggctc aaggtggaca tctacccctc catgcagctc
      121 ggcggccggc agccgcaact cttcgatcag gcccgggatg gcgtggtcga cattgtctgg
      181 acggtggcgg gctcgacgcc cgggcgcttc ccgcgtctgg aggtgttcga gctcccgttt
      241 ctctcccatg ccatcggcga gaagacggcg ccggcggtgt gggagttcta cgagaccttt
      301 gccaaggacg agctcaagga cgtcaagccg ctggccgtct ggggctttgg caagggcatc
      361 ctgttcacca aggacgtggt catcaagacg ccggccgacg ccaagggcct gaagctacgc
      421 gcctccaacc gccagaccaa cgacgccttc gccctgctgg gagcccaacc ccaaagcatt
      481 ccaccccccg gcgtgccgga agcgctcgcc aagggcgtcg tcgacggcgt cgtctttccc
      541 tacgatgccg tgatcccctt caagctcgat gagctcacca accgggtgag tgagttcggg
      601 ggcgaccgca ccttttataa ctcggtccta ctgttcgtga tgaacaaggc caaatacgac
      661 ggcctgcccg acgacctgaa gaaggtgatc gatgccaact cgggcgccgc gctgtcgcgg
      721 gacctgggtc gcaagtggga tgagtgggat caaatcggcc gcgccgccgt caaaaagcgc
      781 aacacgccga tccatgtcat cgacggcgcc gacctcgccg cctggaagac cgcgaccgag
      841 ccgatcttcg ccaaatggat cgaggagcgc gacaaggccg gcgacaaggg tgccgacctc
      901 gtcagggccg cgcaggcgct ggtggccaag tactcccagt gaaccccgcg gcgccgatag
      961 agaggcccgc gccagccccg cacggccgcg tcggccggct gcttgaggcc ctctgcggcg
     1021 cggtggccat gctgggcggc gtgctgctga tcggcatcat gctgatttcg agcctctccg
     1081 tgctggggcg cgggttgtcg ctcctggtcg gggcgcggat ttccggcatc cccggggatg
     1141 tcgagatcgt gcagctcggt tgcgccgtgg cgatatttgc ttttctgccg atctgccagc
     1201 tgaagcgcgc caacgtgctg gtgggggccc tgaccaagca cctgcccgtg cgcgcccgcg
     1261 ccagcttcga ccttgcggcc aatgcgctct ttctcctgct gacgttcctc ttggctttcc
     1321 agctgggcgt cggcacgcgc gagaagttcg ccaaccacga cacgaccatg gtgctgcgca
     1381 ttcccgaggg ttgggcctat gccgtggcgc tggtgtttgc ctggctgctg gtgctgacga
     1441 ccgcctatac cgcggcgcgc agcgcggcgg agatccgccg caatcgcgcc attggcccga
     1501 gcccggcggg ggagcgttga gcgggtccca tgtcgaattt cgcggccggg ctttcgagct
     1561 ttgtgctcct gttaatgctc ttggcgctgc gggtgccgat cggcgttgcc atgctagcgg
     1621 tcggcgtcgg cggctttatc tggatccagg gcctggcgcc gctcttgagc ttcctcaaga
     1681 ccacgccgtt tgagaccttc gccggctatt ccttctccgt cattcccttg tttctgctga
     1741 tgggcaatgt cgcctcgggc tccggcctct cggcgcggct gtttcgcggc gccaatgcgc
     1801 tgctcggtca ccatcgcggc ggcatggcca tggcggcggt cggggcctgc gctgccttcg
     1861 gcacgatctg cggctcctcg ctggcgacgg ccgccaccat gggccaggtg gcgctgcccg
     1921 agatgcgcaa gtacggctac tccggcgcgc tttccaccgg cacgctcgct gccggcggca
     1981 cgctcggcat cctggtgccg ccctcg
//
LOCUS       DASTMT010000156         1824 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_744172, whole genome shotgun sequence.
ACCESSION   DASTMT010000156 DASTMT010000000
VERSION     DASTMT010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 1824)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1824)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1824
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_744172"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..582
                     /locus_tag="VFR00_03460"
     CDS             <1..582
                     /locus_tag="VFR00_03460"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU0158342.1"
                     /translation="ARMDDPRSRPSETGDFARLAMPRRTAAFNLAYWMLGNREEAEDV
                     VQEAYLRGFRAFAGFKGADIRPWLLMIVRNAALTALDLRKRSNNVILLSEDVWASSGN
                     NAEDIAATTPSPEAQVIAASERQALMSALAKLPLKYREVIVLREMEGLSYLEIAETTG
                     MAIGTVMSRLSRARAELRRTLASALHPQKQNAV"
     gene            complement(646..1629)
                     /locus_tag="VFR00_03465"
     CDS             complement(646..1629)
                     /locus_tag="VFR00_03465"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEU0158343.1"
                     /translation="MQVLGAVLAVLSAATFALTNALGRRGVITGTPVQGMVLSIPVGL
                     ICFLLVALLTGALESLGRISLGAIAALSVAGVIHFVIGRYCNFRASQAAGVNLTAPVL
                     QLNAVVTLALAVVVLREPFTVLQMLGGILMVTGSLVTQSASQAAGQGKRTAAPRFTPR
                     IAAGFFFASIAALMYGTSPIIVRQALLDVGPLSGVAGGSIAYAGATVAVTLGLCIPSL
                     RRNVMQVRRDNARWFVYSGIFVAAAQGFLYSALAVAPIMLVVPLLQLSLVFRFLFALL
                     LNPLHELFGAMVVFGSVISIVGASAVSIDTDLILRALALPPALAGFLNAKF"
BASE COUNT          376 a          588 c          604 g          256 t
ORIGIN      
        1 gcccgcatgg acgacccacg atctcggcca agcgagacag gcgatttcgc gcgacttgcc
       61 atgccgcgtc ggacggccgc cttcaacttg gcctactgga tgctgggtaa tcgtgaggaa
      121 gccgaagacg ttgtgcagga agcctacttg cggggctttc gcgcctttgc cggcttcaaa
      181 ggcgcggaca tcaggccgtg gttgttgatg atcgtgcgca acgccgcgct gacggcgctc
      241 gacttgagaa agcgcagcaa taacgtgatc ctgctgagcg aggacgtctg ggcatcgagc
      301 ggcaacaacg ccgaggacat tgccgcaacg accccgagcc cggaagccca ggtgatcgcc
      361 gcaagcgagc gccaagccct gatgtcggcg ctcgcgaagc ttcccctcaa ataccgcgaa
      421 gtcatcgtgc tgcgcgaaat ggaggggctc tcttacctcg aaatcgccga gaccacgggc
      481 atggccatcg gcaccgtgat gtcacgcctc tcgcgcgctc gcgccgagct gcgccgaacc
      541 ctggcgtcgg ctttgcaccc tcagaagcaa aatgccgtct agagccgcgc gccagccggg
      601 cgcgctcgct gccatcgccg gtcggcgcgg gcaacggggt caggctcaga atttcgcgtt
      661 caggaagccg gccagcgccg gcggtagcgc cagcgctcgc aggatcagat ccgtgtcgat
      721 ggagacggca gacgctccga cgatggagat gacggacccg aacacgacca tggcgccaaa
      781 aagctcatgc agagggttca acagcagcgc aaacaaaaag cggaaaacca aggagagctg
      841 caacagcggc accaccagca tgatcggcgc gacggcgagg gcggagtaga ggaagccctg
      901 cgccgcggcc acgaaaatgc ccgagtaaac gaaccagcgg gcgttgtcgc ggcgaacctg
      961 catcacgttt cggcgcaagg aggggatgca taggccgagc gtaacggcca ccgtcgcccc
     1021 cgcataggcg atcgagccgc cggccacgcc gctgagcggt ccaacgtcga gcaacgcctg
     1081 gcgcacgatg atcgggctcg tgccatacat gagcgcggcg atcgaggcga agaagaagcc
     1141 cgcggcaatg cggggtgtaa acctcggcgc ggcggtccgc ttgccttggc cggccgcttg
     1201 tgaggcgctc tgcgtcacga gggagccggt gaccatcagg atgccaccca gcatctgcag
     1261 caccgtgaag ggctcgcgca gcacgaccac cgccagggcc agcgtaacga ccgcgttcag
     1321 ctgcaaaacc ggtgccgtca aatttactcc cgccgcctga ctggctcgaa agttgcagta
     1381 gcgcccgatc acaaaatgga tgacgccggc gacggaaagc gcagcaatgg ctccgaggct
     1441 gatacgcccc aggctctcga gcgcgcccgt cagcagcgcc accaacaaaa agcaaatgag
     1501 acccacgggg atactgagga ccatgccttg taccggcgtg ccggtgatca cgccccggcg
     1561 gccgagggca ttggtgaggg caaaggtagc tgccgacagc actgcaagca cggcgcccaa
     1621 gacctgcact gatctgctcc tcggtaggct ctccgcccac cgcgcaccgt ttttgctcgt
     1681 gcggctggcc ttgggctcat tgccgggcaa gctaaccggc gctcggggta agccgtcaag
     1741 ggtgtgcgga tgcgcggccg ccatgccgat taggagcagg cgggcaggag caggcgggca
     1801 cctttgcgag gcttgcgcgg caac
//
LOCUS       DASTMT010000157         5802 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_1725251, whole genome shotgun sequence.
ACCESSION   DASTMT010000157 DASTMT010000000
VERSION     DASTMT010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 5802)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5802)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5802
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1725251"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..374
                     /locus_tag="VFR00_03470"
     CDS             <1..374
                     /locus_tag="VFR00_03470"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020095847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU0158344.1"
                     /translation="PVADVAGVWQRADIFVLASRGGEGLPRALLEAAASARPLVVSDV
                     PGNRHFVRDGIEGLLVPPGDVGALSAALRRLAEDRELRVALGAAARRRLLEGFTEAHV
                     RAALRASYLDMLGEHTAEDLR"
     gene            511..1086
                     /locus_tag="VFR00_03475"
     CDS             511..1086
                     /locus_tag="VFR00_03475"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019172958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEU0158345.1"
                     /translation="MAQQSFTGKRAFDVVAAACGLILLAPLVAACALAVRLSSPGPAI
                     FRQTRIGKDERPFTCYKLRTMHLQTAHLPTHEMQANAVTSIGRSLRRLKLDELPQLWN
                     VLKGEMSFVGPRPCLPTQVELIRARRRLGLAGLVPGITGVSQVAGIDMSDPERLAHSD
                     ARYVEDISIGTDLALIWQAVAGAGRGDRVRA"
     gene            complement(1110..2054)
                     /locus_tag="VFR00_03480"
     CDS             complement(1110..2054)
                     /locus_tag="VFR00_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011652795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEU0158346.1"
                     /translation="MRVAITGATGFVGNALVRFLARQTPWQLTAFSRRAWPAPAPQLR
                     SVVIGDLAGAPWPADALADTEAIVHAAAMTSSGPPPAPARLHAVNVAATLQLARQAAS
                     AGVKRFVFLSSIKVNGEETAPSKPFTATAAPAPEDAYGQSKLEAERGLRELAKTTGLE
                     VVILRPSLIYGPQVGGNFAHMVQWVARGIPLPLAAVRNKRTLLALPNLLDLIKTALVS
                     PRAANQTLLAGDADDVSTPQLLEAIADALGRPPRLFPVPPALLAGAASLLGKREMARK
                     LLGSLQLDIQDTRARLGWDPPLTTRQALTETVKTFRAG"
     gene            complement(2135..4165)
                     /locus_tag="VFR00_03485"
     CDS             complement(2135..4165)
                     /locus_tag="VFR00_03485"
                     /inference="COORDINATES: protein motif:HMM:NF014740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HEU0158347.1"
                     /translation="MQRGRMASVPQAVPGATRPQAAAPQKWVRWLAFRLVELDRYPKR
                     AILAANDFILFNFALWLAMCLRLGELFVPPSAPVFGVLTAAPLIGVATCFQLGVYRMV
                     TRFIGTRGFTLAAGAVGLSALYWSLLLYLSGIYSIPRSVIILYPVLATVLIWLSRQAA
                     MSLLRRGGVDIPSQAPGHERPVLIYGAGTSGVQLLEALQTTGGYRVVGFVDPRRTLAR
                     QYVAGLRVSSPARLASLIRENGVEEVLLAMSRAQRRERRAALQELEQLKVRVRTLPAM
                     EDLATGRVAVSDLRPVEADDLLGRDPVRPDPAVLARNIAAKAVMVTGAGGSIGSELVR
                     QIIALNPKSLVLFENSESQLYQIDLQAEALCQTARARGEAAPHIVSVLGSVCDAAAVR
                     RAIEGNEVETIYHAAAFKHVPLVEHNPVAGLRNNTFGTATLAEVAAACGVERFVLVST
                     DKAVRPTSIMGASKRLAEMILQARAAEGRGRTVFTMVRFGNVLDSSGSVVQRFRRQVE
                     AGGPVTVTHPGMIRYFMSIPEAAGLVIQAGAMASGGDVFVLDMGEPVKIDDLARTMIR
                     LMGREVRDEDNPDGDIAIEYVGPRDGEKLYEELLIGENVIPTEHPRILRSREPFLAKA
                     KLEPLLDELAATLEQDSAEAIKATLARAVENYRPAQHHLNGSEPFDDGAMAA"
     gene            complement(4756..4887)
                     /locus_tag="VFR00_03490"
     CDS             complement(4756..4887)
                     /locus_tag="VFR00_03490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0158348.1"
                     /translation="MPRGDDRDPDAGAAEPTWRSTLRAASIFLLVTASVVIAFYGLK"
BASE COUNT          978 a         1971 c         1926 g          927 t
ORIGIN      
        1 ggcccgtggc ggacgtcgcc ggggtctggc aacgtgccga cattttcgtg ttggcctcgc
       61 gcggcgggga aggtttgccg cgcgccctcc tggaggccgc cgcctcggcg cgacctctcg
      121 tcgtctccga cgtaccgggc aatcgccact ttgtccgaga cggcatcgag ggtctcctcg
      181 ttccgcccgg cgatgtcggc gcgctcagcg ccgcgttgcg tcgcttggca gaagaccgcg
      241 agctgcgcgt ggcgctcggg gccgccgcgc gccggcgcct gctcgaaggc ttcaccgaag
      301 cgcacgtgcg cgcggccttg cgcgcaagct acctggatat gctgggcgag cacacggcgg
      361 aggacctcag atgaggcgtg cagcgctttg taccttggcc gtgtcgccgc gctatagtct
      421 cgccgggcgt gcgcacgcgg ccgggaccgt tggcgccccg cggtgccaga cagggtagca
      481 tcgtttgcga agaacgaggg ggaggggggc atggcgcagc agagctttac cggcaagcgt
      541 gccttcgatg tcgtggcggc cgcctgcggg ctcatcctgc tagcaccgct ggtcgccgcc
      601 tgcgcgctcg cggtccgtct ctcctcgcca gggccggcca tctttcgcca aacccgcatc
      661 ggcaaagatg agcggccctt tacctgctac aagctgcgca ccatgcactt gcaaacggcc
      721 cacctgccca cgcatgagat gcaggccaac gccgtcacct cgatcggcag gtcgctccgg
      781 cgcctcaaac tcgacgagtt gccccagctc tggaacgtgc tcaagggcga gatgagcttt
      841 gttggaccgc ggccctgctt gccgacgcag gtggagctga tccgggcgcg ccgtcgcttg
      901 gggctcgccg gcctggtgcc cggcatcacc ggcgtctcgc aagtcgccgg catcgatatg
      961 tcggacccgg aacgtttggc ccacagcgac gcgcgctatg tcgaggacat ctcgattggt
     1021 accgacctgg cgctgatttg gcaggcggtg gccggtgcgg gacgcggcga ccgcgtgcgc
     1081 gcctgagccc agcgccacac cggcgcgctt tagcccgcgc gaaacgtctt gacggtctcg
     1141 gtcagggcct gccgcgtcgt cagcggtggg tcccagccga gccgcgcgcg cgtgtcttgg
     1201 atatcgagtt gcagcgaccc caacaacttc cgcgccatct cgcgcttgcc caacaaggag
     1261 gcggcgcccg ccaggagcgc tggaggcacc gggaagaggc gcggcggccg gcccaaggcg
     1321 tcggcgatcg cctcgagcaa ttgcggcgtc gagacatcgt ccgcgtcgcc tgcgagcaag
     1381 gtctggttgg ccgcgcgcgg gctcaccagc gccgtcttga tgagatcgag caaattgggc
     1441 agcgccagca aggtgcgctt gttgcgcacg gccgccagcg gcaggggaat gccgcgcgcc
     1501 acccactgca ccatgtgggc gaagttgccg ccgacctggg ggccatagat gaggctcgga
     1561 cgcaggatga caacctcgag gcccgttgtc ttggcgagct cgcgcaagcc gcgctcggcc
     1621 tcgagcttgg attggccgta ggcatcctcg ggcgcgggtg cagcggtggc cgtgaacggc
     1681 ttactcggcg ccgtttcctc gccgttgact ttgatggagc tcaaaaagac gaagcgcttg
     1741 acgcctgccg aggccgcctg ccgcgccaat tgcagcgtcg ccgcgacatt gaccgcgtgc
     1801 aaccgcgctg gggctggcgg cggcccgctg ctcgtcatcg ccgccgcgtg cacgattgcc
     1861 tcggtgtccg cgagcgcgtc cgcgggccag ggggcgccgg cgagatcgcc gatgacaacc
     1921 gagcgcagct gcggggctgg cgccggccag gcgcgccggc tgaacgccgt cagctgccag
     1981 ggcgtttgcc gtgccaagaa gcggacgagg gcattgccca caaagcccgt tgccccggtg
     2041 atcgccaccc gcatcgccgt tgacttaccc caattcttga gctcgtggcg gctagcgcca
     2101 gcgcaccgcc gcgcccaagc tggctcaggc gggctcaggc ggccatcgcg ccgtcgtcga
     2161 agggctcgct gccattcagg tgatgttggg ccgggcgata attttctacc gcgcgcgcga
     2221 gcgtcgcctt gatcgcctcc gccgagtcct gctcgagggt ggcggccagc tcgtcgagca
     2281 ggggttccag cttggccttg gcgaggaacg gctcgcgact gcgcagaata cgggggtgct
     2341 cggtggggat gacattttcc ccgatcagca gttcctcgta gagcttctcg ccgtcgcgcg
     2401 ggccgacgta ctcgatggca atgtcaccgt cgggattatc ctcatcgcgc acctcgcgcc
     2461 ccatcaagcg gatcatggtg cgcgccaggt cgtcgatctt gaccggctcg cccatgtcga
     2521 gcacgaacac atcgccgccc gacgccatgg cgccggcttg aatgacgagc cctgccgcct
     2581 cgggaatcga catgaaatag cggatcatgc cggggtgcgt gacggtcacg ggccctccgg
     2641 cctcgacctg acgccggaag cgctgcacga ccgagcccga gctatcgagc acattgccga
     2701 agcgcaccat ggtgaatacg gtgcgtccgc gtccctcggc ggcgcgggcc tgcagaatca
     2761 tttccgccaa gcgcttgctg gctcccatga tgctggtcgg tcgcacggct ttgtcggtcg
     2821 acaccagcac aaaccgttcc acaccgcagg cggcggcgac ctccgccagc gtggcggtgc
     2881 caaaggtatt gttgcgcagg ccggcgaccg gattgtgctc gacaaggggg acgtgtttga
     2941 aggccgccgc gtgatagatg gtctccacct cgttgccctc gatggcccgg cgcacggcgg
     3001 cggcatcgca caccgagccg agcaccgaga cgatgtgggg cgcagcctca ccgcgggcac
     3061 gcgcggtctg gcacagcgct tcggcctgca gatcgatctg atagagctgg ctctcgctgt
     3121 tttcgaacag cacgaggctc ttagggttca gcgcgatgat ctggcgcacg agttccgagc
     3181 cgatggaacc gccggcgccg gtcaccatca cggccttggc ggcaatgttg cgcgccagca
     3241 cggccgggtc gggacgaacc ggatcgcggc ccaggagatc gtcggcctcg acgggccgca
     3301 aatcgctgac cgccacgcgt cccgtcgcaa gatcttccat ggccggcagc gtgcgcacgc
     3361 gaactttcag ttgctccaat tcctgcaggg cggcccgccg ttcgcggcgc tgcgcgcgcg
     3421 acatggccag caacacttcc tccacgccat tctcgcgaat gaggctcgcc aagcgcgcgg
     3481 gcgaggagac cctgaggccc gccacgtatt gacgcgccag cgtccggcgc ggatcgacaa
     3541 aaccaacgac ccggtagcca cccgtcgttt gcaaggcctc gagcagctgc acgccgctgg
     3601 tgccggcgcc atagatcagc accgggcgtt cgtgcccggg cgcctgggag ggaatatcga
     3661 cgccgccacg gcgcagcaac gacatcgccg cttgccggct tagccaaatc aagaccgtcg
     3721 ccagcacggg ataaagaatg atcaccgagc gggggatgct gtagatgccg gacaaataga
     3781 ggagcaggct ccagtataaa gccgacagtc cgacggcgcc ggcggcgagg gtgaagccac
     3841 gcgtgccgat gaaacgcgta accatgcgat agacgcccag ctggaaacag gtcgccacgc
     3901 cgatcagggg cgccgccgtc aaaacaccaa acacgggtgc gcttgggggc acgaacagct
     3961 cgcccaagcg caggcacatg gccagccaaa gcgcaaagtt aaagagaata aagtcgttgg
     4021 cggcgaggat ggcgcgcttg gggtagcggt cgagttccac cagccgaaag gcgagccagc
     4081 gcacccattt ctggggcgcc gccgcctgcg gcctggttgc tccaggcacc gcctgcggca
     4141 cgcttgccat gcgcccccgt tgcattttta tccctcactt cccaagcaac cgccgcggca
     4201 ttgcccctgg ccgtcgtgca tgatggaagc gttacgcaaa agtccggttc agtaggaaac
     4261 acgaccgttg gcacaaaaag gaaaacgcag gaggtgtaaa gcacccgtcg caaaatcccg
     4321 actcgccaaa aagcttagca cattgccgcc acgactccac aacccgaaga tgtcattgcc
     4381 ttttcttggc cgttggcggg cgccgcaggc gatcgcggtt atccccgctt ttaacaacga
     4441 cgcaggcccg cggtgcacca cctcgctcgt gcagcggtcg gtggtgttgc tagaccgcca
     4501 ctcgacgccg cgctttaaaa aaaagacacc gtccccgtaa gagacggtgc aaaaaatgtc
     4561 acatcccgcg tcagggttag ggaagcaaac gcgggacgcg cgcgcagcct tagcacgagt
     4621 cgcacgtctg tcagagcctc tcccgcgcgc ctggcgcgga ccgcttttgt tgccaggatc
     4681 tcgagcggat aagcgcattc ctgcgggcca agcgcgaggg gatcgggcag gcgcgcgtga
     4741 ggcaggcgaa gtgcgctact tgaggccgta aaaggcaatg accaccgagg cggtcaccag
     4801 cagaaagatc gaagccgccc tcagggtact gcgccacgtc ggctcggctg cgccggcgtc
     4861 cgggtcccta tcgtcccccc tcggcatggc gttccctttc ggccagcatc agcgcgcggc
     4921 tgcggcggct gagcactcgg gcgtcgggtt cgcaatcgca ccgcctgaac tcagctcgcg
     4981 cagaccctcc cagcatctct acgctagacc agaacccgca ccgggccaac gccgtttttg
     5041 gcttttgccg ggagccggca gcgccatggc tcggcgtttg ggtcgcggca catgccccgc
     5101 gcgcacgcgt gccgcgcgca agccaaacgt taagagctgc cgcgctggac agggatctcg
     5161 cgccgacttt atttgtacgc cgcgccgcct aggacacgtt cgccgcgctt cgcaccggta
     5221 cgcgggcgca accggcgcgg ctcaagaggc cttacagagt tcgtgccaag cgccggcaac
     5281 ggcctcggac ttttccgcac acgcctggca gcgaccggtg gcggcataga cctcgcgttc
     5341 cccctctttc gtcagggagc cacaggcacc gcagcgaggg cctaagctct tcgccttcgc
     5401 tttccaatcg tcccatgaca tgatttcgcg gaatctggct tcggccacag tgatctccgt
     5461 cgctggtgtg cgcgtgagcc acgcgcatct tcacgccaat cacgagatac gctgagcgcc
     5521 aggcaaaccc cagcacgcaa aaaaatgggc tggagcacct tgccaaagct cgggtcacgc
     5581 cgccacgcgg caacccggga gggcgccaca gacaacggca ggagcgcgca actattcccg
     5641 ggcggcgccg cgatctggcg gctcgagccg cggcattccc tcgggggatg gcggaaggcc
     5701 tttagtattg gcgcgctggc gccgccgctg cggtcatccg tgcctcagcg cggccgcgcg
     5761 gcagcgagcg ccagacgttt ctttctgtgt gggctgataa gt
//
LOCUS       DASTMT010000158         4857 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_3368098, whole genome shotgun sequence.
ACCESSION   DASTMT010000158 DASTMT010000000
VERSION     DASTMT010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4857)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4857)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4857
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3368098"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..939)
                     /locus_tag="VFR00_03495"
     CDS             complement(<1..939)
                     /locus_tag="VFR00_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008195739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEU0158349.1"
                     /translation="MDLGMTTKVKPLVEKVRAMVRDEIMPLEAEYEAEVATGDRWTYT
                     KRQEEIREGLKAKARERGLWNFWLTASDKGYGLTTVEYAYLAEEMGWSRLAPETFNCS
                     APDTGNMEVIERYGTPAQKEKWLTPLLEGKIRSAYIMTEPNVASSDATNLALDAKLEG
                     DEWVLNGEKWFASGAGDPRCKIYIVMVCTDPTADKHKRHSQIFVPAGTPGVEILRPMK
                     VYGDDDAPHGHMHIRFKNARVPKENLILGRGRGFEVSQGRLGPGRIHHCMRAIGQAER
                     ALELLCKRSLSREAFGKPLAQLGANYDIIAESRMKIE"
     gene            1200..1346
                     /locus_tag="VFR00_03500"
     CDS             1200..1346
                     /locus_tag="VFR00_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aa3-type cytochrome c oxidase subunit IV"
                     /protein_id="HEU0158350.1"
                     /translation="MAVDPSGGHPAMDYPEHLRTYSGFLKATVTLIIGVALLLLFMLF
                     YLVP"
     gene            1413..2552
                     /locus_tag="VFR00_03505"
     CDS             1413..2552
                     /locus_tag="VFR00_03505"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008746 - NAD(P)+ transhydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HEU0158351.1"
                     /translation="MVTIAVTAEGQGEPRVAVSPETVKKLTALGASVRVQQGAGSRSR
                     FSDEALKAAGATVNADASGTLEGADILLKVRRPSLAEVGALKRGAIVAAMLAPFDDRP
                     GLEALAGAGAALLSMEFMPRITRAQSMDVLSSQANLAGYKAVVDAAAMFGQAMPMMMT
                     AAGTVPAAKVFIMGVGVAGLQAIATARRLGAIVSATDVRPATKEQVASLGAKFIAVED
                     EEFKQAQTAAGYAKPMSPEYQKKQAELIANHIKAQDIVITTALIPGRPAPKLISTAMI
                     ESMKPGSVIVDLAAERGGNTELTQPDKVVEHKGVSIAGPLNLAGEVPVNASALYAKNL
                     LAFLETMFDKKDKVLKVNWDDEIIKGTLVAKDGRIVHPSLQQQGA"
     gene            2707..3030
                     /locus_tag="VFR00_03510"
     CDS             2707..3030
                     /locus_tag="VFR00_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013420505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-translocating transhydrogenase family
                     protein"
                     /protein_id="HEU0158352.1"
                     /translation="MAVGGADVSPFIYQFMVFVIAIFVGYYVVWSVTPALHTPLMSVT
                     NAISSVIVVGALLAVGVTLIGAGSGLAKTFGFVALTMASVNIFGGFLVTQRMLGMYKK
                     REPKR"
     gene            3050..4450
                     /locus_tag="VFR00_03515"
     CDS             3050..4450
                     /locus_tag="VFR00_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011440335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HEU0158353.1"
                     /translation="MHPNLVALLYLAAGVLFIMALRGLSSPESSRRGNMYGMVGMTIA
                     VLTTLGQLSDTSPLTWALIVCGLAIGGGIGATIARRIPMTSMPELVAAFHSLVGLAAV
                     FVAAGALYAPEAFGIGHPRSIAAASLLEMSLGVAIGAVTFSGSVIAFAKLSGRMSGKP
                     IILPARHFINAGLAVLLLLLIFWFCGSGGDPILFWAITAVALVFGVLIIVPIGGADMP
                     VVISMLNSYSGWAAAGIGFTLGNIALIVTGALVGSSGAILSYIMCKGMNRSFVSVILG
                     GFGGETAGPAAGAEQKPVKIGSAEDAAFLLKNSGKVIIVPGYGMAVAQAQHALREMAD
                     HLKGAGVEVKYAIHPVAGRMPGHMNVLLAEANVPYDEVFELEDINNEFAQADVAYVIG
                     ANDVTNPAAEEDKSSPIYGMPVLQVWKAGTVMFNKRSLASGYAGIDNPLFYRDNTMML
                     LGDAKKMTEEIGKAMD"
     gene            complement(4462..4674)
                     /locus_tag="VFR00_03520"
     CDS             complement(4462..4674)
                     /locus_tag="VFR00_03520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0158354.1"
                     /translation="MTGQNTYAKPAAAAPEVRRQRPSNLVPILVVLFAILSALVLFVM
                     DPSGSPGPQPNYRADAPPPPQSAPPR"
BASE COUNT          827 a         1571 c         1535 g          924 t
ORIGIN      
        1 ctcgatcttc atgcggctct cggcaatgat gtcgtagttg gcgccgagct gggccaacgg
       61 cttgccgaag gcttcgcgcg acaatgatcg cttgcacaag agctccagcg ctcgctcggc
      121 ctggccaatc gcgcgcatgc agtggtggat gcggccggga cccagccgac cctgcgagac
      181 ctcgaagccg cgcccacgcc caagaatcaa gttttccttg ggtacgcgcg cgttcttgaa
      241 gcggatgtgc atgtgaccgt gtggcgcatc gtcgtcaccg tagactttca tggggcgcag
      301 gatttccacc cccggcgttc ccgccggtac gaagatctgt gagtgtcgct tgtgcttgtc
      361 ggccgtcgga tcggtgcaga ccatgacaat gtagattttg cagcgcggat cgccggctcc
      421 agaggcgaac cacttctcgc cgttcagcac ccactcatcg ccttcgagtt tggcgtccag
      481 cgccagattg gtggcatcag aggaggccac gttcggctcc gtcataatgt aggcggaccg
      541 aattttgccc tccagcaagg gcgtgagcca tttctccttt tgcgccgggg taccataacg
      601 ctcgatcact tccatgttgc cggtatcggg ggcggagcag ttgaaagtct ccggcgccag
      661 ccgcgaccag cccatttcct cggcaagata ggcatattcg accgtggtca agccgtagcc
      721 cttgtcactc gccgtcagcc aaaaattcca caggccccgt tcgcgcgcct tggccttgag
      781 gccttcgcgg atctcctcct ggcgtttggt gtaggtccaa cggtcgccgg tggcgacctc
      841 ggcctcgtat tcggcctcca gcggcatgat ctcatcacgc accatggcgc gcactttttc
      901 gaccagcggt ttcacctttg tcgtcatgcc gagatccatg gaggtcctcc tctgcgggtc
      961 cgcccgcata ctgccgtggc cgtgcgcatt ggctgcgcgg acattgaagc atgcccatcc
     1021 aacggagaga aggcggtagc ccgaaggctg cgccgggggg cgcggcggca ggccccaggc
     1081 ttgccgcgat ggcgcttgag tgccggcgcc gggcaagcca taagccgagc aagacttgat
     1141 gcctggcaag gcttcggata ccaagaccgc ggcgcaatca tgagagcgag cgacgaccta
     1201 tggctgtcga cccttccggc ggtcaccccg ccatggacta tcccgagcat ttgcggacgt
     1261 actccggttt tctcaaggcc acggtgaccc tgatcatagg ggtggcgctg ctgctcctat
     1321 tcatgctgtt ttatctggtg ccctagtcgg gcagcacctt ccttgctttt aaatgggcgg
     1381 gacagctccc gtcgcagcat ggggaaaacc tcatggtcac gatcgccgtc accgcagagg
     1441 gacagggtga gcctcgcgtg gcggtgtcac cggagaccgt aaagaagctg acggcgctgg
     1501 gtgccagcgt cagggtgcag cagggcgccg gctcgcgttc gcggttttcc gacgaggcgc
     1561 tcaaggcggc cggtgcgacc gtcaacgccg acgcctccgg tacgctcgag ggggccgaca
     1621 ttctgctaaa ggtgcggcgg ccgtcgctcg ccgaagtcgg agccctcaag cgcggtgcca
     1681 tcgtcgccgc catgctggcc cccttcgatg atcgccccgg ccttgaggcc ttggcgggtg
     1741 cgggtgcggc actgctgtcc atggaattca tgccgcgcat cacgcgggcc cagtcgatgg
     1801 acgtgctgtc gagccaggcc aacctcgccg gctacaaggc cgtggtcgac gcggcggcca
     1861 tgtttggcca agccatgccg atgatgatga ccgccgcggg gacggtgccg gccgccaagg
     1921 tgtttatcat gggcgtcggc gtggcaggct tgcaggcgat cgccacggcg cggcggctcg
     1981 gtgccatcgt ttcggcgacg gatgtgcgcc ccgccaccaa ggaacaggtg gcctcgctcg
     2041 gcgccaagtt cattgccgtc gaggacgagg aattcaagca agcgcaaacc gccgccggtt
     2101 atgccaagcc gatgtcgccg gagtaccaga agaagcaggc cgaactgatc gccaatcaca
     2161 tcaaggctca ggacatcgtc attaccacgg ccctcattcc tgggcggccg gcacccaaat
     2221 tgatatctac ggccatgatc gagagcatga agccgggttc ggtgatcgtc gacctggcgg
     2281 cggagcgcgg cggcaatacc gagctgaccc agcccgacaa ggtggtcgag cacaaaggcg
     2341 tctcgatcgc cggcccgctc aatctggcgg gcgaggtgcc cgtcaatgcc tccgccctct
     2401 atgccaagaa tttgctggcc ttcctcgaga ccatgttcga caagaaagac aaggtgctga
     2461 aggtgaactg ggacgacgag atcatcaaag gtacgttggt ggccaaggat ggccgaattg
     2521 tccacccgag cctgcagcaa cagggggcat gagatgaggc gcttgcttgc caggccttcg
     2581 agccccgtcg ccgagcgcca caccgacgcg tgcgggcgaa accctgccgg ctggcgcggc
     2641 ccccgtcccc cgcgcgctcc tctcggcgcg ctcaccttgc tgttggctct ttttcctgca
     2701 ccggccatgg ctgtaggcgg cgccgatgtt agccccttta tctatcaatt catggtgttc
     2761 gtcattgcca tcttcgttgg ctactacgtg gtgtggagcg tgacgccggc gctgcacacg
     2821 cccttgatgt cggtgacaaa cgccatttcc tcggtgatcg tggtcggcgc gctgctcgcc
     2881 gtcggcgtga ccttgatcgg cgccggcagc gggctcgcca agacgttcgg gttcgtggct
     2941 ttgacgatgg cgtccgtaaa catcttcggc ggcttcctcg tcacccagcg catgctcggc
     3001 atgtacaaga agagggagcc gaagcgctga tgcgctgagg gggcggccca tgcatccaaa
     3061 tctcgtcgcc ttgctttacc tggccgcggg cgtcctattc atcatggcgc tgcgcggctt
     3121 atcgagcccc gagagctcgc gtcgcggcaa catgtacggc atggtcggca tgaccatcgc
     3181 cgtcttgacc acgctaggac aactcagcga cacctcgccg ctcacctggg cgctaattgt
     3241 ctgcggcctc gccatcggcg gcggcatcgg cgccaccatc gcccggcgca tcccaatgac
     3301 gtcgatgccc gagctggtgg ccgccttcca ctctctcgtc ggcctcgccg ccgtcttcgt
     3361 tgcggcgggg gcgctgtacg cgccggaggc cttcggcatc gggcacccgc gcagcatcgc
     3421 cgccgccagc ctgctcgaaa tgtcgctcgg tgtcgccatc ggcgccgtca ccttttcggg
     3481 atcggttatc gccttcgcca aactgtccgg ccgcatgtcc ggcaagccga tcattctgcc
     3541 ggcccgacat ttcatcaacg ccggcctggc cgtcctcctc ctgctactga tcttctggtt
     3601 ctgcggctcg ggcggcgatc ccatcttgtt ctgggccatc accgcggtgg cgctggtatt
     3661 cggcgtcctc atcattgtgc cgatcggcgg cgcggatatg cccgtcgtca tctcgatgct
     3721 caactcttat tccggttggg ccgccgccgg catcggcttt acgctcggca acatcgcgct
     3781 gatcgtgacg ggggcgctgg ttggttcctc gggcgccatt ctgtcctaca tcatgtgcaa
     3841 ggggatgaac cgctcctttg tctccgtcat ccttggcggc ttcggtggcg agacggcggg
     3901 acccgccgcc ggcgccgaac aaaagcccgt aaaaatcggc tcggccgaag acgccgcgtt
     3961 cctgctcaaa aattccggca aagtaatcat cgtgccgggc tacggcatgg ccgtggcgca
     4021 ggcccagcac gccttgcgcg aaatggccga ccatctcaag ggcgcgggcg tcgaggtcaa
     4081 atacgccatc catcccgtgg ccggacgcat gcccggacac atgaacgtgc tcctagccga
     4141 ggccaacgtt ccctacgacg aagtgttcga gttggaggat atcaacaatg agtttgcgca
     4201 agccgacgtt gcctatgtga tcggcgccaa cgatgtgacc aatccggccg ccgaggagga
     4261 taaatcctcg ccgatttacg gcatgcccgt gcttcaggtc tggaaggccg gcaccgtcat
     4321 gttcaacaag cgctcgctgg cttccggcta tgccggcatc gacaatcctc tgttctatcg
     4381 cgacaacacc atgatgttgc ttggcgatgc caagaagatg accgaggaga tcggcaaggc
     4441 gatggattga ggagccgccc cctagcgggg cggggcagac tgtggcgggg gcggcgcatc
     4501 ggcccgatag ttgggttgtg gcccaggcga accgctgggg tccatgacga acagcaccag
     4561 cgcggaaagt atcgcgaaca gcacgacgag tatgggcacg agattactcg gccgctgacg
     4621 cctgacctcg ggcgcagcgg cagcaggctt ggcatacgtg ttctggccgg tcatagcggc
     4681 tccgagctgc ctaaaagcct aactcggggc gcgcgccacc agttcccaac catggccagt
     4741 ccggaggcgc agctggcagg aaaagcagcc ggcaggaaaa aagaccgctg tcgccgttcg
     4801 ctaaaccccg ggccgcttct gccgggcgcg gtagtcggac ggacgctcaa cgtcgcc
//
LOCUS       DASTMT010000159        11563 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_3040793, whole genome shotgun sequence.
ACCESSION   DASTMT010000159 DASTMT010000000
VERSION     DASTMT010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 11563)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11563)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11563
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3040793"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..484)
                     /locus_tag="VFR00_03525"
     CDS             complement(<1..484)
                     /locus_tag="VFR00_03525"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0158355.1"
                     /translation="MQKHIAQPVHATQPLWLVVLAAGLICAIAMGLRQVMGLYLPPMT
                     TELGIGREPFSTAMAIANLTWGLGAVLAGMVADRYGAGRVVVSGVLATMVGMYVMYAA
                     RNGVDLMLSGILLGIGVSGTGIPSLVGAVGRAAPPDKRTSAIASLGMASGIGGFVAFP
                     Y"
     gene            complement(621..1550)
                     /locus_tag="VFR00_03530"
     CDS             complement(621..1550)
                     /locus_tag="VFR00_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEU0158356.1"
                     /translation="MATALTIAHLSDVHLGPIRGFTPRYWNLKRLTGYWNWLSTRRDF
                     YQRSVLDRLVGDLKAQRPDHIVVTGDLANIGLPQEHIDALAWLHALGPPDHVSVIPGN
                     HDIYARQRRDPGTRRWAAYMQSNAEGERYLLGEGPASEFPFVRRLEDVALIGLNSAVP
                     TPPLIAWGSAGRKQLARLARVLDSAGRAGLFRLVLIHHPPLPGQASPTRGLRDAAALQ
                     GVLREHGAELVIHGHNHLNMLSLEASASGATIPIVGVASASLARRHGREGLARYNLYR
                     IAGPPWRITLAGRGLDDPDGGIVELERRSLLAT"
     gene            1648..2109
                     /locus_tag="VFR00_03535"
     CDS             1648..2109
                     /locus_tag="VFR00_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013417784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEU0158357.1"
                     /translation="MVRNIVLIRVLQRYWRWRRGLTMGAQAVVLDAAGRVLLVRHGYQ
                     PGWHFPGGGVEAGETIADAVARELGEEAGITLAAPAELFGIYANFAAFPGDHIALFVV
                     RSWQQSEVPAPNREIREQGFFAPERLPAGTTKAARRRLAEVLGGAQQSPFW"
     gene            2332..4413
                     /locus_tag="VFR00_03540"
     CDS             2332..4413
                     /locus_tag="VFR00_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006023040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEU0158358.1"
                     /translation="MQQRVLHTAKSACPHDCPSACALEVRVLPGNRIGRITGASDNRY
                     TQGVVCAKVARYSERVEHPERLLYPLRRRGAKASGQFERVTWEAALGEIAERFVTIAQ
                     RSGSEAIWPYHSGGNMGVLQRYGLDRLRNPLRYSRQHTTICVTPAESGWLAGVGKLVG
                     PDPCEMEAADLIVIWGGNPVSTQVNVMTHVARARKTRASKLVVVDVYRTPTVEQADIA
                     LIINPGSDAALALAIMNVLLTEELVDRRYLARYTDFDAETERHILSRTPQWAAAITGL
                     AAKDIIAFARLYGQTRKSFIRVGFGFTRTRNGASAMHAVSCLPALTGAWTERGGGAFF
                     LSFDKNQWGLDTSLINASDAIDPGVRLLDQSRIGAVLCGEAEALGRGPPVEAMLMQNA
                     NSATVAPSTAKVLKGLARDDLFLVVQEQFLTPTARHADIVLPATMFVEHDDIYYGLGH
                     THLTFGPKLVEPRGEARPNSYTVRALQGLLGVNHASVAMSDLELLDHALARGGLGRFA
                     AAAEVGWIDRALSFEAAHFLTGFPQADGKFHFKPDWSQVGPGHAAMPRVADWSPAYER
                     AGPSHPYKLVCPPARHFLNSTFSETPTSRAKELAPCVRIHPDAARREGVEEGARVRIG
                     NERGSVVVPAKLDAGQQEMTLIVEGIWPAEAFLERRAINTLIGDDAVPPNGGAPFHDT
                     AVWLERIRELA"
     gene            4516..4968
                     /gene="mce"
                     /locus_tag="VFR00_03545"
     CDS             4516..4968
                     /gene="mce"
                     /locus_tag="VFR00_03545"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR03081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HEU0158359.1"
                     /translation="MKVKRIEHVAIAVKSLAKAREIFEDKLGLTFEYEEWLPQYQTKL
                     AMFPVGESYLELLETKRADSETAKWIAEHGEGLFHICLEVDDIVAALAELKAKGVKLI
                     DAEPRLGHANSRIAFLDPSSTNDVLIELVELPPEAGKAPGAARDQLAP"
     gene            complement(4988..5740)
                     /locus_tag="VFR00_03550"
     CDS             complement(4988..5740)
                     /locus_tag="VFR00_03550"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU0158360.1"
                     /translation="MTEPNDLFADLDFRHRRVLITGAANGFGAAIAQRFAQHGASLVL
                     ADIEAAPLAAIAVRLNAEAHSFDQADAGSVERLVEAAGAIDILVNNAGILVAKPLLET
                     SLEEVRRLIDTDLVGVIRLMQLVGRGMVERRRGVIVSMGSQTAFAGGENRAVYAAAKA
                     AISQLTRAAAVEWGPHGVRVVCLAPGRALTRMTETTASPHYTGDRGLQRVPLGRWGTP
                     EEISKLVLLLASDAAGYVTGETLIADGGYVIG"
     gene            complement(5737..7797)
                     /locus_tag="VFR00_03555"
     CDS             complement(5737..7797)
                     /locus_tag="VFR00_03555"
                     /inference="COORDINATES: protein motif:HMM:NF014585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HEU0158361.1"
                     /translation="MSLRLDPITLEVLTQALIATVREMRATVCRTASSVAIYDAKDFS
                     CGLFAPDSQVIAQSEDIGSHVVPMPWSVRAAMQKLGDTLGEGDVILMNDPYAGGTHLN
                     DVTIIYPVFRDGRLIFFPAVRAHWADVGGMVPGSMSGKATEIYQEGIRIPPLKIFEAG
                     KTNQAALDLLLANMRVPDERLGDFQASLAACRVAEKRIHEICSRYGVDTLLEAVRLDL
                     DRAEARMRASIAAVPDGTSYYEDYLETFIGDRFEPLLLPLALTVKGDRMIADFTGASP
                     QVPFPVNSTAAVSAAGVLITVKSIFDPQAPLNQGSFRPIEVITPPGTIVNVERPAPAG
                     SHGEIRKRVIATMVGALAQMVPDKVAGDLCRTSFHNLIGGFDKRAGREWVHYEWSAGG
                     NGAFAEDDGPSAIATIDWGDLVTVQSTEVIETRMPLLVESSRLAVDSGGPGSTRGGLS
                     MQRTLRLLASDARYSLLSDGAVVPAFGVRGGFSGVPVAAWIQRNGRTEDFSTPGKVAG
                     HPLAEGDAVIVRSAGGGGYGDPLDRDAERVAADLSDGYISPAAAREVYGVVFDAAQGI
                     DAAATNALRQRLRQARLGLDARLTAEAFEAGAVSRRRVWRLNPNDAALANVGEDDVVE
                     LDSGRAAPLRGWARLDASLRPGTVSIDARGLAILKAAEGETLTLRRVRAAVASPSVIA
                     SKRE"
     gene            complement(7803..9821)
                     /locus_tag="VFR00_03560"
     CDS             complement(7803..9821)
                     /locus_tag="VFR00_03560"
                     /inference="COORDINATES: protein motif:HMM:NF014071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HEU0158362.1"
                     /translation="MLWLGVDVGGTFTDLVLFDGVAGTLRILKTPSSPGNQADGILLG
                     LDRLQVKPASLERVVHGTTVATNTALEGNGARLAVLVTAGHRDVLVVGRGNRLAMYNI
                     KARPARPLVPRSQVIEIGERLSVDGSVLKALDEAEVTRVAERLAAEGIEAVAVCFLHA
                     YANAAHEARAAAVLAKHLPRATVTSSAEVLPEFREYERFSTTALNAYVAPRMRRYLGD
                     LRRRLAERGVGAPLAIMTSNGGSLPAHRVEAMPVLSMLSGPAAGVIAAGHIGALSGFS
                     HLITCDMGGTSTDVCLLRDGNYEMTTEGRVGAFPLKLRQIDINSIGLGGGSIATLGTG
                     GFLSVGPRSAGAVPGPACYGRGGTEPTVTDANVVLGRLGVEQALGGEISLDAAAAERV
                     LGALAHRVGLGTPQMAEGILRLAAVSLAGAIKEVSVMRGIDPRDFALLPFGGAGPLHA
                     AEIADELGMRAVVVPPLPGNFSALGLLIADVRRDFVRTRVSPTSTTSMADVEAALGEL
                     MRNAAADLAADGFTAERQRFAPSLDMRYAGQSFELSVPVAIDARSIAALERSFAEIYA
                     TRYGAATGRPMEIVSYRLAAWGLSDKPQLPRLDPGGRSLAGARRGARGVYFAGGQRHV
                     PILDRERLPLRESIDGPVLIEEAGTTTVVPAGWSAELDPVGCLVLRRS"
     gene            complement(9834..10622)
                     /locus_tag="VFR00_03565"
     CDS             complement(9834..10622)
                     /locus_tag="VFR00_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009735518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEU0158363.1"
                     /translation="MVTQTDILVACDVGGLATVTLNRPAKRNAVSLAMWRRLGDIFTE
                     LAARDDVGVIILTGTGGHFCAGADISEFASVRADVASGRLYEAINEAASLALRDCPKP
                     TIAAISGYGMGGGCALALACDLRVGDATTRMGIPAARLGVVYGALDCALLHRQVGLAN
                     AKHILFSGRAFAAQDCLKMGLLDSIAGDTALAGAQALAQQIVGNAPLSLAGSKYILEA
                     LSAGTAAARDAEITGLIEAAMASADYREGARAFLEKRPPSFSGR"
     gene            10710..>11563
                     /locus_tag="VFR00_03570"
     CDS             10710..>11563
                     /locus_tag="VFR00_03570"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEU0158364.1"
                     /translation="MASALERLKVIDLSSHLSGPYCAMLLADHGADVIKIERPGSGDD
                     ARRMPPFMGGESTPFMIWNRNKRSVVLDLKQDKDKAQLLALIDTADVLIENFRPGTLD
                     RLGLGWSTLSRRNPRLIYAAISGFGQTGPYRERGGFDLVTQGMSGLMSVCGPSEGPPH
                     RLPIAISDVAAGMHLAFGILCALEARHHTGRGQLVETSLLESALSFGVYEAAHVAATG
                     ATPPRLGQAHRGSSPYQVFETADGWITLGAAQDNFWRRLCDLLGQPELTEDRRFASNG
                     DRVRNNAAL"
BASE COUNT         1994 a         3752 c         3973 g         1844 t
ORIGIN      
        1 tatagggaaa ggcaacaaag ccgccgatgc cgctcgccat gccgaggctg gcgatcgcac
       61 tcgtgcgttt gtcgggcggt gccgcccggc cgacggcgcc caccagcgag ggaatgccgg
      121 tgccgctgac gccaatgccc aagaggatgc cgctgagcat caggtcgacg ccgttgcggg
      181 cggcatacat aacgtacatc cccaccatgg tcgcgagcac gccggacacg accacgcgtc
      241 ccgcgccgta gcggtcggcg accatgccgg ccagcaccgc acccaatccc cacgtcagat
      301 tggcaatcgc catcgccgtg gagaacggct cgcggccgat gccgagctcg gttgtcatgg
      361 gcggcaggta aagtcccatc acctggcgca ggcccatggc aatggcgcaa atgaggccgg
      421 ccgccagcac gaccagccac agcggctgcg tggcgtggac gggctgggca atgtgctttt
      481 gcattctcag tcaccttgtt gttcgggggc gcaccctaca ggcagcgcgg cggcgcggca
      541 cggcaaatta cgcgcgaatt gcccgaatat ttctcaggtc aggcatgaca tttatttatg
      601 tctcgagccg cgcggttggc tcatgtcgcc aagaggctgc gacgttcgag ttcgacgatg
      661 ccaccgtcgg gatcatcgag accgcgcccg gcgagcgtga tgcgccacgg cggccccgcg
      721 atacggtaga gattgtagcg ggcgagccct tcgcgcccat ggcggcgggc aagggaggcc
      781 gatgccacgc ccacgatagg gatcgttgcc cccgacgcgg acgcctcgag gctcagcata
      841 ttgagatggt tgtgcccatg aatgacgagt tcggcgccgt gctcgcgcag gacgccttgc
      901 agcgcggccg catcgcgtaa gccgcgcgtg ggggaggcct ggcccggcag cggcggatgg
      961 tggatcagca cgaggcggaa taggccggcg cggccggcgc tgtcgagcac gcgcgcaagc
     1021 cgggcgagtt gtttcctgcc ggcgctcccc caggcgatca aggggggcgt gggcacggcc
     1081 gaattgaggc cgatcagcgc gacatcctcg aggcgacgca caaagggaaa ctccgacgcg
     1141 gggccctctc ccaggaggta gcgttcgccc tcggcgttcg attgcatgta ggcggcccag
     1201 cggcgcgtgc cgggatcgcg acgctgccgg gcatagatat cgtgattgcc cggaatgacg
     1261 ctgacgtggt cgggcggccc gagcgcgtgg agccatgcca aggcatcgat gtgctcctgt
     1321 ggcaggccga tgttggcaag atcgccggtg accacgatgt gatctgggcg ctgggctttg
     1381 aggtcgccaa ccaggcgatc gagcacgctg cgctggtaga agtcccggcg cgtgctcagc
     1441 cagttccagt agccggtcag ccgcttcagg ttccaatagc gcggcgtgaa gccgcggatg
     1501 gggcccaggt gtacgtcgga aagatgagcg atggtaagcg cggtggccat tgccgggcga
     1561 ttggtagccg cttcgcgcag ccttgccaaa tgcgccgcag cggttgcaga gaaggagcgt
     1621 gcaaggcgaa gcctgacgac ggcagggatg gtccgcaaca tcgttctcat tcgggtgctg
     1681 cagcgctatt ggcgctggcg tcgtgggctc accatgggtg cgcaagcagt ggtcctggac
     1741 gcggcgggcc gcgtgctttt ggtgcgacac ggctaccagc cgggctggca cttccccggt
     1801 ggcggcgtcg aggcgggcga gacgattgcc gacgcggtgg cccgcgagct cggcgaggag
     1861 gccggcatta cgctggcagc acctgcggag ctgttcggca tctatgccaa tttcgccgcc
     1921 tttcctggcg accacatcgc gctgttcgtg gtgcgcagtt ggcagcagtc cgaagtgccc
     1981 gcgccgaacc gggaaatccg cgagcagggc ttctttgcgc cggagcgttt gccggcgggc
     2041 acgaccaagg cggcacgccg caggctggcc gaggtgctgg gcggtgcgca gcagagcccg
     2101 ttctggtaag cgctctggcc aagcgcgggg gccttgcgtt tcccgcacgc gtgcctcgat
     2161 ctcgggcctc agccgcgcgc gcctgtcagc aaggccgcgc gcatcccacg tgcaaagtgg
     2221 cccgcgtccg cgtggcgaaa cgctattcgc gcaatcgtgg tttttcccct aggatcgccg
     2281 tcgaggccgc caaagccgcg gcagcctgcg cgtggccaag aggatgctgg catgcagcaa
     2341 cgcgttcttc acacggccaa atcggcctgc ccgcatgatt gtccgagtgc ctgtgccctc
     2401 gaggtgcggg tgctgcccgg caaccgcatc ggtcgcatca ccggggccag cgacaaccgc
     2461 tacacgcagg gcgtcgtgtg cgccaaggtc gcccgctaca gcgagcgcgt cgagcacccg
     2521 gagcgactgc tctacccgtt gcggcggagg ggagccaagg cttcgggaca attcgaacgc
     2581 gtgacctggg aggccgcgct tggcgaaatt gccgagcgct tcgtgaccat cgcgcagcgc
     2641 tcggggtcgg aagcgatctg gccgtaccac tcgggcggca atatgggcgt gctgcagcgc
     2701 tacggtctcg atcgcctccg caatcccctg cgctactcgc gccagcacac cacaatctgc
     2761 gtcaccccgg ccgaatcggg atggctggcc ggcgtcggca aactggtggg gccggatccc
     2821 tgcgagatgg aggccgcgga cttgatcgtg atctggggtg gcaatcccgt ttccacccag
     2881 gtcaatgtca tgacccacgt tgcccgcgcg cgcaagacgc gcgccagcaa gctggtggtg
     2941 gtcgacgtct atcgcacacc gaccgtcgag caggccgaca tcgccctgat tatcaatccg
     3001 ggctccgacg ctgcccttgc cttggccatc atgaacgtgc tcctgaccga ggagctggtc
     3061 gatcgcagat accttgcccg ctatacggat ttcgatgccg aaaccgagcg ccatattctg
     3121 agccgcacgc cgcaatgggc cgcggcaatc accggccttg cagccaaaga catcatcgcc
     3181 tttgcgcgtt tgtatggcca aacgcgcaag agctttatcc gggttgggtt cggcttcacg
     3241 cgcacgcgca acggcgcctc tgccatgcat gcggtgtcct gcctgccggc gctgaccggt
     3301 gcctggacgg aaagaggcgg gggcgccttc tttctcagct tcgacaagaa ccagtggggg
     3361 ctcgatacct ctctcatcaa cgccagtgat gccatcgatc cgggggtgcg gttgctcgat
     3421 caatcgcgga tcggcgccgt gctgtgcggt gaagccgagg cgctcggccg cgggccgccg
     3481 gtcgaggcta tgctcatgca gaacgccaac tccgcgacgg ttgcgccgag cacggccaag
     3541 gtgctgaagg gtctggcgcg cgacgacctc ttccttgtgg tgcaggagca gttcctgacg
     3601 ccgacggcgc gccacgccga catcgtgctg ccggcgacca tgttcgtcga acacgacgac
     3661 atctactatg gtctcggcca cacacacctg acgtttgggc cgaaactggt cgagccgcgt
     3721 ggggaggcgc gccccaacag ttacacggtg cgcgcgttgc aaggcttgct cggcgtaaat
     3781 catgcaagcg ttgccatgtc cgatcttgag ctgctcgatc acgcgttggc ccgcggcggg
     3841 ctcgggcgct tcgccgcggc ggcagaggtc ggctggatcg accgcgcctt gtcgttcgag
     3901 gccgcgcact tcttgacggg cttcccgcaa gcagacggaa aattccactt caagccggac
     3961 tggagccagg tggggcccgg ccatgcggcg atgccgaggg tcgccgactg gtcgccggct
     4021 tacgagcgtg ctgggccttc gcatccttat aagctcgtgt gtccgccggc ccgccatttc
     4081 ctcaacagca cgttttccga gaccccgacc tcgcgcgcca aggaactggc gccctgcgtg
     4141 cgcatccatc ccgacgcggc gcgacgcgag ggtgtcgaag agggagcgcg ggtgcgcatt
     4201 ggcaacgagc gcgggtccgt ggtcgtgccg gcgaagctcg acgcgggcca gcaggaaatg
     4261 accttgatcg tggaggggat ctggccagcc gaggcctttt tggaacggcg cgccatcaac
     4321 acgctgatcg gtgatgatgc cgtgccgccc aacggcgggg ctccctttca cgacaccgcc
     4381 gtgtggctgg aacgcatccg cgagttagcc tgaaccccgc gtggccgcag aacccatggc
     4441 gcagcctgcc gttgtcacgc ccgcaatgga tcttgaataa cgccgctaac ccctaatggc
     4501 gcgaggagct cgcttatgaa ggtcaagcgt atcgaacacg tggccatcgc ggtcaaaagc
     4561 ctggccaagg cgcgggagat tttcgaggac aagctcggct tgaccttcga atacgaggag
     4621 tggctaccgc aataccaaac caagctcgcc atgttcccgg tgggcgaaag ctacctcgag
     4681 ctgctcgaga ccaagcgtgc cgacagtgag accgcaaagt ggatcgccga gcacggcgaa
     4741 gggctctttc acatctgcct tgaggtcgac gacatcgtgg cggcgttggc cgaactcaag
     4801 gccaagggcg taaaactcat cgatgccgaa cctcgtctcg gccatgccaa ctcccgcatc
     4861 gcctttctcg atcccagttc caccaacgat gtcttgatcg agctggtcga actgccgccg
     4921 gaggcgggca aagcgccagg cgcggcgcgc gatcagctgg cgccatgagg ctcgcgcgcg
     4981 ggcccgatca gccgatcacg taaccgccat ccgcgatcag ggtttcgccg gtgacgtagc
     5041 cggccgcgtc ggaggcgagc aacaggacca acttgctgat ctcctcgggc gtgccccagc
     5101 ggcccaaggg cacccgctgg aggccgcggt cccccgtgta gtgaggcgag gccgtggtct
     5161 cggtcatgcg cgttaaggcg cggccgggcg cgagacagac cacgcgtacg ccgtgcggcc
     5221 cccactcgac ggcggcggcg cgcgtgagct gcgagatggc ggctttggcg gcggcgtaga
     5281 cggcccggtt ttcaccacca gcaaaggcgg tctgcgaacc catggaaacg atgacaccgc
     5341 gccggcgctc caccatgccg cggccgacca gctgcatcag ccggatgacg ccgacgaggt
     5401 cggtatcgat cagccggcgc acctcctcga gcgaagtttc gagcagcggt ttggcgacga
     5461 gaatgccggc attgttgacg aggatatcga tggcgccggc ggcctcaacc agccgctcca
     5521 ccgagccggc atcggcttgg tcgaagctgt gggcctcggc gttgagcctg acggcaatgg
     5581 cggcgagtgg agctgcttcg atgtccgcca gcaccaggct ggccccatgc tgggcgaagc
     5641 gttgcgcgat cgccgcaccg aagccgttcg ccgccccggt gatgagaacg cgccggtgcc
     5701 taaaatcgag gtccgcaaaa agatcgttcg gctcggtcat tctcgcttgc tcgcaatcac
     5761 tgatggggat gccacggcgg cgcgtacccg ccgcagcgtc agcgtctcgc cctcggcggc
     5821 cttgaggatg gcaaggcctc gcgcatcgat gctgacggtt ccggggcgca ggctggcatc
     5881 gagccttgcc cagccgcgca gcggggcggc ccgtccacta tcgagctcaa cgacgtcgtc
     5941 ctcgccgaca ttggcgaggg cggcgtcgtt tgggttgagc cgccagacgc ggcggcggct
     6001 gacggcgccg gcctcgaacg cctcggcggt caagcgggcg tcgaggccga ggcgggcctg
     6061 gcgcaagcgt tggcgcagcg cgttggtggc tgcggcatcg atcccttgcg cggcgtcgaa
     6121 gaccacacca tagacctcgc gggccgcggc gggtgagatg tacccatcgc tgaggtcggc
     6181 ggccacgcgc tccgcgtcgc ggtcgagcgg gtcgccataa ccgccgccgc cggccgaccg
     6241 aacgatcacc gcgtctcctt cggccagcgg atggccggcc actttgccgg gggtggaaaa
     6301 atcctctgtc ctgccattgc gctggatcca ggccgccacg gggacgccag aaaagccgcc
     6361 gcgaacgccg aaggccggca ccacggcgcc atccgacagc agggaatagc gggcatcgct
     6421 ggccaacagg cgcaaagtgc gctgcatgga caggccgccg cgcgtgctgc cgggcccgcc
     6481 cgaatcgacg gccagccgcg agctctcgac cagcaacggc atgcgcgttt cgatcacttc
     6541 ggtggactgc accgtgacga gatcacccca atcgatggtg gcgatggcgc ttggcccatc
     6601 gtcctcggcg aaggcgccgt tgccgccggc tgaccactcg tagtggaccc actcgcggcc
     6661 ggcgcgctta tcgaagccgc cgatcaaatt gtggaaggag gtgcggcaga gatcgccggc
     6721 gactttatcc ggcaccattt gcgccagcgc gccaaccatc gtggcgatca cgcgcttgcg
     6781 gatctcgccg tgcgaaccgg caggtgccgg ccgctcgaca ttgacgatgg tccccggcgg
     6841 cgtgatcacc tcgatcggcc gaaaggagcc ttggttcaag ggtgcttgtg gatcgaagat
     6901 ggacttgacc gtgatcagca caccggcagc cgagaccgcc gccgtcgagt ttaccggaaa
     6961 tgggacctgc ggggaggccc ccgtgaaatc ggcaatcatg cgatcgcctt taaccgtcag
     7021 tgccaggggc agcagcagtg gctcgaagcg gtcaccgata aaagtctcca gatagtcttc
     7081 gtagtagctg gtgccatcgg gtaccgccgc aatcgaggcg cgcatgcggg cctcggcgcg
     7141 atcgaggtcg aggcgaacgg cttcgagcag cgtatcgacg ccatagcggc tgcagatttc
     7201 gtggatacgc ttttccgcca cccgacaggc ggccaagctc gcttggaagt cgccgagccg
     7261 ctcgtcgggc acgcgcatgt tggcgagcag cagatcgagc gccgcctggt tggtcttgcc
     7321 agcttcgaaa atcttgagcg gcgggatgcg aatgccttct tggtagattt cggtcgcctt
     7381 gccggacatg ctgcccggta ccatgccgcc gacgtcggcc caatgcgccc gcaccgccgg
     7441 gaagaagatg agacgcccgt cgcgaaacac cggatagatg atggtgacgt cgttgaggtg
     7501 ggtaccgccg gcatagggat cgttcataag aatgacgtcg ccttcgccca gcgtgtcgcc
     7561 cagcttttgc atggcggccc gcaccgacca cggcatcggc accacgtgcg agccgatgtc
     7621 ctccgactgg gcgataacct ggctatccgg tgcaaagagc ccgcaggaaa agtccttggc
     7681 atcgtaaatg gcaaccgagc tcgcggtgcg gcacaccgtg gcgcgcattt cgcgcacggt
     7741 cgcaatcagc gcctgcgtca ggacttcgag cgtaatggga tcgaggcgca acgacatggg
     7801 cttcagctcc ggcgcagcac gagacagccc acgggatcga gctccgcgct ccagccggca
     7861 ggaacgacag tggtggtgcc ggcttcctcg atcagcacgg ggccgtcgat gctttcgcgc
     7921 aggggcagcc gctcccggtc caagattggg acgtgacgct ggccgccggc gaaatagacg
     7981 ccccgggccc cgcgccgagc gcccgcaagc gagcgtccgc cgggatcgag ccgcggcaac
     8041 tgcggcttat ccgaaaggcc ccaggcggcg agacggtagc tgacgatttc catcggccgt
     8101 cccgtggcgg cgccgtagcg ggtcgcatag atttcggcga aggaacgctc gagcgcggca
     8161 atgctacggg cgtcaatggc gacggggacg gagagctcga aggattggcc agcgtagcgc
     8221 atgtcgaggc tcggggcgaa gcgttgccgc tcggccgtga atccgtcggc ggcgaggtcg
     8281 gcggcagcat tgcgcatcag ctccccgagc gccgcctcga cgtcggccat cgaggtcgtg
     8341 ctggtcgggg acacacgcgt gcgcacgaag tcacgccgca cgtcggcgat caataggcca
     8401 agggcggaga aattgccggg tagcggcggc accacgaccg cgcgcatgcc gagctcgtcg
     8461 gcgatctcgg cggcgtgcag ggggcccgcg ccgccgaagg gcagaagcgc gaaatcgcgc
     8521 gggtcgatgc cgcgcatgac ggagacttcc ttgatggcgc cggcaaggga gacggcggcg
     8581 aggcgcaaaa ttccctccgc catttgcggc gtgccgagac caacgcggtg cgccaaggcg
     8641 ccgagcacac gttccgcggc ggccgcatcg aggctgatct ctccgcccag cgcctgctcg
     8701 acgccgagcc ggccgaggac cacgttggca tcggtcaccg tcggttcggt gccgccgcgg
     8761 ccgtaacagg cgggccctgg cacggccccg gcggagcgcg ggccgacgga aagaaagccg
     8821 ccggtgccga gcgtggcaat gctgccgccg ccgagcccga tcgaattgat gtcgatctgg
     8881 cgcagcttca gggggaaggc gccgacccgg ccttccgtcg tcatctcgta gttgccgtcg
     8941 cgcaagaggc agacgtcggt ggaggtaccg cccatgtcac aggtgatcag gtgcgaaaag
     9001 ccactgagcg cgccaatgtg gccggccgcg atcacgcccg ccgccggccc cgacagcatg
     9061 gagagcaccg gcattgcctc gacgcggtgg gcagggagcg agccgccgtt ggaggtcatg
     9121 atcgccagtg gtgcaccgac gcccctctca gccaagcgcc gccgcaggtc gccgaggtag
     9181 cggcgcatac gcggtgcgac ataggcattg agcgccgtgg tggaaaagcg ctcgtattcg
     9241 cggaactcgg gcaagacctc ggccgagctc gtcacggttg cccgggggag gtgcttggcg
     9301 agcacggccg cggcgcgcgc ctcatgggcg gcgttggcat aggcgtgcag gaagcagacc
     9361 gcaaccgctt cgatgccctc ggcggccaag cgttcggcca cccgcgtcac ctccgcctcg
     9421 tcgagcgcct tgaggaccga gccatcgacg ctgagccgct cgccaatctc gatgacctgc
     9481 gaacggggca ccaacggccg cgcggggcgc gccttgatgt tgtacattgc cagccgattg
     9541 ccgcggccca ccaccagcac gtccctgtgc ccggcggtca ccaacacggc aaggcgcgcg
     9601 ccgttgccct cgagcgcggt attggtggca accgtcgtac catgcaccac gcgttcaagg
     9661 cttgcgggtt tcacctgcag acgatcgagc ccgagtaaga tcccgtcggc ttgattgccg
     9721 ggggaggagg gggttttcag gatccggagc gtgccggcaa ccccgtcgaa caggaccaga
     9781 tcggtgaagg tgccgccgac gtcaacgcca agccaaagca tgccgggccc gtcctagcgg
     9841 ccgctaaagc tgggcgggcg tttttcgagg aaggcgcggg cgccctcgcg atagtccgcg
     9901 ctcgccatgg cggcctcgat caggccggta atctccgcgt ctcgagctgc agccgttcca
     9961 gcgctcaagg cttcgagaat gtattttgac ccggcaaggg agagcggggc attgccgacg
    10021 atttgctgcg ccagcgcctg cgcgccggcc agcgccgtgt cgccagcgat actgtcgaga
    10081 agccccatct tgaggcagtc ctgggccgca aaggcgcgac cgctgaaaag tatgtgcttg
    10141 gcgttcgcaa gaccgacctg gcggtgcaga agggcgcaat cgagggcgcc gtagaccacg
    10201 ccaagccggg ccgcgggaat gcccatgcgc gtcgtggcgt cgcctactct gaggtcgcag
    10261 gccagcgcca gggcgcagcc gccgcccatg ccgtaccccg agatcgcggc gatggtcggc
    10321 ttggggcagt cacgcagcgc caggctggcc gcctcgttga tcgcctcgta aagccggccc
    10381 gaggccacgt cggcgcgcac gcttgcgaac tcggaaatat cggcgccggc gcagaagtgg
    10441 ccgcccgtgc cggtcaaaat gatgacgccg acatcgtcgc gggcagcgag ctcggtgaag
    10501 atgtcaccga gccgccgcca catcgccagt gagacggcgt tgcgcttggc tggccggttc
    10561 agcgtgacgg tggcgaggcc gccaacgtcg caagcaacga gaatgtctgt ttgagtgacc
    10621 acgattgacc ctgtcagaaa gcacgtgcgc acggcgccgg aagtgggcac aataaacaaa
    10681 aatcttggca agggaaaaag tgaaagccga tggcaagcgc gctggaacgc ctcaaggtca
    10741 ttgatttgtc tagtcacctc tcgggaccct actgtgccat gctgctggcg gaccatggcg
    10801 ccgatgtcat caagatcgag cgtcccggga gcggcgacga tgcccggaga atgccgccct
    10861 tcatgggcgg cgaaagcacc ccgttcatga tttggaaccg gaacaagcgt tccgtcgtgc
    10921 tcgatctgaa gcaggacaaa gacaaggcgc agctgctggc cctgatcgat actgccgacg
    10981 tgctgatcga aaacttccgg ccgggcaccc tcgaccgttt gggcttgggc tggagcacgc
    11041 tttcgcgccg caatccgcgc ttgatctatg ccgccatttc cggcttcggc cagacgggtc
    11101 cctatcgcga acgtggcggc tttgatctcg tgacccaggg catgtccggc ctgatgagcg
    11161 tgtgtggccc aagcgagggg ccgccgcatc ggctgccgat cgccatttcc gatgtggccg
    11221 ccggcatgca tttggccttc ggcattcttt gcgcgctgga ggcgcgacat cacacgggac
    11281 gcggccagct ggtggagacc tcgctattgg aatcggcact ttccttcggg gtttatgagg
    11341 cagcgcatgt cgccgccacc ggagcgacgc cgccgaggct tgggcaggcg catcgtggca
    11401 gctcgcccta tcaagtgttc gagacggcgg acggctggat cacgcttggc gcagcgcaag
    11461 ataatttctg gcgccgcttg tgcgatctgc tggggcagcc agagctgacc gaggataggc
    11521 gctttgccag caatggtgac cgggtccgca acaatgctgc gct
//
LOCUS       DASTMT010000160         6224 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_4025384, whole genome shotgun sequence.
ACCESSION   DASTMT010000160 DASTMT010000000
VERSION     DASTMT010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 6224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6224)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6224
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4025384"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(148..224)
                     /locus_tag="VFR00_03575"
     tRNA            complement(148..224)
                     /locus_tag="VFR00_03575"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(188..190),aa:Pro,seq:tgg)
     gene            648..1046
                     /locus_tag="VFR00_03580"
     CDS             648..1046
                     /locus_tag="VFR00_03580"
                     /inference="COORDINATES: protein motif:HMM:NF018717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HEU0158365.1"
                     /translation="MNQYFGVALGMVAGVVVGAAAITGLHAQAKPPVLLVTEIDVTDP
                     EAYGKEFAPKAQATIKAAGGRFIAIGGTAGAGAKPITAMAGTAPKRVTIQAWESLDAL
                     NAWYNSADYQAALKIGEKYATFRRYAIEGQ"
     gene            1410..2414
                     /locus_tag="VFR00_03585"
     CDS             1410..2414
                     /locus_tag="VFR00_03585"
                     /inference="COORDINATES: protein motif:HMM:NF014653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HEU0158366.1"
                     /translation="MTGRLRLSVAEARELAEGALVGIGYAPDEARIIADHVIDAALCG
                     YEYSGLAKILNVPESEHFRLPRRPMQVLRETAISLAFDGGNNVGMLALFHAAQASIDK
                     AAAHGIALVSVTDAWMSGRSAYYVEMIAKAGLVAIHTASSSRLVAPPGGTRAILGTNP
                     IAIAVPSAHGPIVLDMGTSAYMMTELMLRERLGESLPEGVALGPDGEPTTDPARARLG
                     ALLPFGGYKGFGLALMVQALGVLAGAGSEAESDYGYLFIAFRPDLLGPAERFQHGVAR
                     LIEKVKATPRQNNVAEIRIPSERAFRSRERLLREGLEIDRPVFEALMALRAGSAATCG
                     "
     gene            2509..2973
                     /locus_tag="VFR00_03590"
     CDS             2509..2973
                     /locus_tag="VFR00_03590"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEU0158367.1"
                     /translation="MLTADMKRIIQEQRLGFVATAAADGTPNLSPKGTFVVLDDVTIA
                     FGEIRSPGTLRNLRDNPRVEVNFVDPFARKGYRFAGTAHVVERGSASFPALLERLKST
                     LAPRMRAIVTITVTRALPLTSPAYDDGTEEPAIRRSWTRHFRSLQPSQRFEE"
     gene            complement(3246..3584)
                     /locus_tag="VFR00_03595"
     CDS             complement(3246..3584)
                     /locus_tag="VFR00_03595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0158368.1"
                     /translation="MIQSWLTIAGLVLDFAGFTLLLREWWLAFFNEGRQIEAEEQLER
                     MRHLRGLRPASSGADPFAALNKIQDDHAVRSARAAHKSAMAARRSVFLLAALLIAAGF
                     VLQLAGAWPT"
     gene            3863..5572
                     /locus_tag="VFR00_03600"
     CDS             3863..5572
                     /locus_tag="VFR00_03600"
                     /inference="COORDINATES: protein motif:HMM:NF017773.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU0158369.1"
                     /translation="MLQDLLARPALAPHLLRIFILLLAGLLLLDGGRAWTQGSAERWI
                     VLATRAIDTRAGRATIDVRKAKGAFRAVRLAVKSGALTVTHVEVAYSDGTVQKLRRSL
                     TLREEQKTPAFARRANDAFIDTITLSFRTAAGELESAVLEVAGLQGPEGAVAVRTPPA
                     VEPPPSPAAPGPDKTTRQGAKGGEETDRAPEGTGHRARKAARQSEPKPRQEAEPPPPA
                     PVVRGIGEPWDVVPVFYGTDRGREARAAGTGYGTERARRLELGRALVTVPKAHQLPNI
                     ERPWVYRLPFTQIVLHSEPEDPRLHFTLKDVRPLSREEFLKLIQERLEASSIYKDHAL
                     VFVHGFNTSFEYALYRTAQIAYDIKFDGAPLLYSWPSMGNMTLQDYSHDRESSGQAEP
                     YMREFLELVARETGAKSVSIIAHSMGNQLLLPALRDLKRMAPSDITISQVILAAPDVD
                     RDAFENIAHELAGVSRGVTMLAASNDLALDISRRFWGGVPRAGDVPAGGPIVTDGIDT
                     IDITAVSTKVFALNHSGYADTNALLSDIQLLIQTGERPPDKRVPILERVKTAKGDYWR
                     YPR"
     gene            5919..>6224
                     /locus_tag="VFR00_03605"
     CDS             5919..>6224
                     /locus_tag="VFR00_03605"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEU0158370.1"
                     /translation="MTVAEILAAKGSKVITISPSDTITTLCECLREKRIGAAIVSRDG
                     KTVEGVISERDVAYGLAVHKDELPDLPVAALMTKAVITCSPNDPVALVASTMLARNIR
                     "
BASE COUNT         1132 a         2030 c         2019 g         1043 t
ORIGIN      
        1 cgcgcatggg tggatgtgac gcaatcacag tctgatgcga acgggatcca cgctccaaaa
       61 cggcagcagc gaaacgaatg atgacctgcc gacagaatga acgcctgaca gaatggaaag
      121 tgcgccgctc taacccattg aatttgttgg tcggggcagc aggattcgaa cctgcggccc
      181 cctgctccca aagcaggtgc tctaccaggc tgagctatgc cccgagccgc aatttccgcg
      241 gcttagtagc agctttcacc cgttctgaaa atggacaaga gaagaacaag tcgacaacac
      301 cagggctcca gaacctctac cggagcgttg gccggtcgat ctgcagcgaa cattcgacgg
      361 cccgcttcgg cctgccgcca tcgaccctca agatttccgc gactgcacgc cgacggagct
      421 gcggaacacg atcacaaatt tggagtggcg cctttggtcg aattttgatc aggcatcgga
      481 gattgcgacc tacgggtata tcagcggcct aggggcagca acgcttttcg aggcgaacct
      541 ttggtctgaa cacgccatgc ggcacccgca atttggacag agcacggact tatcagtacg
      601 atgctctcgg ctcgcccggg gcgagcacta gcgaagggga gaaaactgtg aaccaatatt
      661 ttggtgtggc acttggcatg gttgcgggcg tcgtcgtggg agccgcggcg attactgggc
      721 tgcatgcaca ggccaaaccg ccggtgcttc ttgtaaccga aatcgatgtc acggatccgg
      781 aggcttacgg aaaagagttc gcacccaagg ctcaggccac catcaaagca gcgggcggtc
      841 gctttattgc gatcggcgga accgcgggag ccggggcaaa gcccataaca gccatggcgg
      901 ggacggcgcc gaagcgagtg accattcaag cttgggagag tttggatgcg ttgaatgcct
      961 ggtacaacag cgcggattac caggccgcgc ttaaaatcgg cgagaagtat gcgaccttcc
     1021 ggcgatatgc catcgagggt caataagacg tctcacgcct gagactggcg agaccgccta
     1081 ccggaccgcc accgcgacga accacttgca gtgccgcaga tgggtcgcgg accccgccga
     1141 caaggctggg ccgcgtcccc cgcgcgcctc gcattgcccg ctttggcggg cgggcgggcg
     1201 gcgggggcgg cgcgcgcggc cgttcttgag tgccgcggga gggatttggg cacccctccc
     1261 gtcgggcgcg accggccccg gcggggcgtg gccccaacct ccagaaggaa tggcgagcga
     1321 aagggtgcta gggcgcgatt gcgaggggac ctgcaagcgt taaactgccc gcaggaaaaa
     1381 cgatggcgtc agacaaggag atggtcccca tgacaggccg cctgcgcctc agcgttgcgg
     1441 aggcccgcga gctcgcggag ggcgcattgg ttggcatcgg ctacgcgccc gacgaggcgc
     1501 gcattatcgc cgaccacgtc atcgatgcgg ctctgtgcgg ttatgaatac tcgggcctgg
     1561 ccaagatcct caacgtgcct gaaagcgagc atttccggtt gccccggcgg ccgatgcagg
     1621 tgctgcgcga aacggcgatt tcgcttgcct ttgacggggg caacaatgtc ggcatgctcg
     1681 ccctctttca cgccgcccaa gccagcatcg acaaggccgc ggcgcatggc atcgcgctcg
     1741 tttccgtgac cgatgcctgg atgagcggcc gcagcgccta ctatgtggaa atgatcgcca
     1801 aggccggttt ggttgccatt cacaccgcct cgagctcgcg gctggtggcc ccgcccggcg
     1861 gcacccgagc catcctcggc accaacccca ttgcgatcgc cgtcccatcc gcgcacgggc
     1921 ccatcgtgct cgacatggga acctccgcct acatgatgac cgagttgatg ctgcgcgagc
     1981 ggctgggtga gagcctgccc gagggggtgg cgcttggccc ggacggcgag ccgaccacgg
     2041 atccagcgcg ggcgcggctc ggcgcattgc tgccgttcgg tggctacaaa ggctttggcc
     2101 ttgcgctcat ggtccaggca ctgggggtgc tcgccggcgc cggcagcgag gcggaaagcg
     2161 actatggcta cctgttcatc gcctttcgcc ccgatctcct cggtccggcg gagcgcttcc
     2221 aacacggcgt ggcccgcctg atcgagaagg tcaaggcgac gccgcggcaa aataacgtcg
     2281 cggagatccg tattccctcc gagcgcgcct ttcgctcccg cgagcgcctg ctgcgcgagg
     2341 ggttggagat cgacaggccc gtcttcgagg cgcttatggc cttgcgggct gggtccgccg
     2401 caacgtgtgg ctgagcgaaa tcatggccgg gcagcacgcg cggaggtggc tgaggaagcg
     2461 ttggccaagc ctgcgcaatc gcagaatagg gagcacggga gcgcgcacat gctgactgcc
     2521 gacatgaagc ggatcatcca agagcagcgc ctcgggtttg ttgccaccgc tgccgccgac
     2581 ggcacgccca acctctcgcc caaaggcacc ttcgtcgttc tggacgatgt caccatcgcc
     2641 ttcggcgaga ttcgctcccc tgggaccctg cgcaatttgc gcgacaatcc tcgcgtggag
     2701 gtcaacttcg tcgatccttt cgcgcgcaag ggctatcgct tcgccggcac ggcgcatgtg
     2761 gtggagcgtg gtagcgccag ctttccggcc ctgctggagc gattaaagag cacgttggca
     2821 ccgcgcatgc gcgccattgt cacgatcacg gtgacccgcg cgctgcccct cacctcaccc
     2881 gcctacgacg atggcacaga ggagccggca atccggcgca gctggacacg ccactttcgc
     2941 tctttgcagc caagccagcg cttcgaggag taaccatccg gcaactggca gcccggcggc
     3001 aacgttcttt ttcacgtgag ccaatgccat gaggtagcgc cttgtccgag cagcatgaac
     3061 cgcgagcccc gcaagacagc aatctagcga tcaccatcgc tggcgtggtg gccgtcctcg
     3121 ccaccctggg cgtgctctac atttccggga tgttcaattg accggccggc ccgcgcacgg
     3181 catcgcccgg cgcagggctt gaggacgggc ggcgcggctg gctcgtgcgc cagggcacgg
     3241 cccgcctagg tcggccaggc gccggcaagc tgcagcacga agcccgcggc aatcaatagc
     3301 gccgccaaca ggaaaaccga tcgccgcgcg gccatggccg atttgtgggc cgctctggcg
     3361 ctgcgcacgg cgtggtcgtc ctggatttta ttgagcgcgg caaacgggtc ggcgcccgaa
     3421 cttgccgggc gcaggccgcg caaatggcgc atccgctcca actgctcctc cgcctcgatc
     3481 tgccgcccct cgttgaagaa cgccagccac cactcccgca gcaggagggt aaacccggca
     3541 aagtccagca ccagcccagc gatggtcagc cagctctgaa tcatcgccta acccacgcgg
     3601 acgcaggcca ggcacgcctg gcccggggga tgcgagggcg gattttcgat cgcgtcatcg
     3661 cctgtctcac cggcagcggg ggcccacacc gcctgggaat tatggcgcca tcatgtaggt
     3721 caagcgaaca ctggggttat tcgctgtcgc gaagtcgcgg tccgggcgcg gacggcaagt
     3781 cacgcctccg acacgatgcc gtgccagttg tgctgcgctt tacgcaacga caactgctgc
     3841 tggcgattgc gggacgattg ttatgttaca ggatttgctg gcgaggccgg ccctcgctcc
     3901 tcatctgctg cggatcttca ttttactgtt ggcgggtctg ctgctgctcg acggcgggcg
     3961 ggcctggacg cagggatcgg cggagcgttg gatcgtgctg gcgacccgtg ccatcgacac
     4021 tcgggccggg cgcgccacga tcgatgtgcg caaggcaaaa ggggccttcc gtgcggtccg
     4081 gctcgccgtc aaaagcggcg ccctgacggt gacccacgtc gaggtggcct atagcgacgg
     4141 cacggtgcag aagctgcgcc gatcgctcac gttgcgtgag gagcagaaga cgccagcttt
     4201 tgcccggcgc gccaacgatg ccttcatcga caccatcacg ctgagttttc gcaccgccgc
     4261 cggggagctg gaaagtgccg tcctcgaggt cgccggcctg caaggccccg agggcgccgt
     4321 tgccgtgcgc accccgccgg ccgtcgagcc acccccatcc cccgctgccc caggccccga
     4381 caagaccacg cggcagggag ccaaaggcgg cgaggagacg gatcgggctc ccgaagggac
     4441 ggggcaccgg gcacgtaaag ccgcacgcca gtcggaacca aagccccggc aggaggcgga
     4501 accgccccca cccgcccccg tcgtcagagg cattggcgag ccctgggacg tggtgcccgt
     4561 gttttacggt accgatcgcg gccgcgaggc gcgcgcagca ggcaccggct atgggacgga
     4621 gcgggcgcgg cggctggaac tcgggcgcgc ccttgtcacg gtccccaagg cgcatcagtt
     4681 gcccaacatc gagcggccct gggtctaccg cttgcccttt acccagatcg tgctgcacag
     4741 cgagcccgag gatccgcgct tgcatttcac gctcaaggac gtgcggccgt tgagccggga
     4801 agagttcctg aaactgatcc aggagcgcct cgaggcctca agcatctaca aggatcatgc
     4861 tctggtcttt gtgcacggct tcaacaccag cttcgaatac gccctctacc gcacggcgca
     4921 aattgcctac gacatcaagt tcgacggggc gccgctgctc tacagctggc catccatggg
     4981 caacatgacg ctgcaggatt attcgcacga ccgggagagt tcgggtcagg ccgaacccta
     5041 tatgcgcgaa tttctggagc tcgtcgcccg cgagacgggc gccaaatcgg tcagcattat
     5101 cgcgcacagc atgggcaatc agctgttgct gccggcgctg cgtgatctca agcgcatggc
     5161 gccaagcgat atcaccatct cgcaagttat tctggccgcg cccgatgtgg accgggacgc
     5221 cttcgaaaac atcgcccatg agctcgcggg cgtcagccgc ggtgtcacca tgttggcggc
     5281 cagcaacgat ctggcgctcg atatttcgcg tcggttttgg ggtggcgtgc cgcgcgcggg
     5341 tgacgtgccg gccggtggcc cgatcgtgac cgatggcatc gataccatcg acattacggc
     5401 tgtctccacc aaggtgttcg cgctcaatca ctcgggctac gccgatacca acgcgctttt
     5461 gagcgacatc cagctgctga tccagacggg cgaacgccca cccgacaagc gcgtgccgat
     5521 tttggagcgg gtcaagaccg ccaaggggga ctactggcgc tatccaaggt agccgcggtc
     5581 gcgcgcgcag cgctcgaccg ccctagcgtc gaccttaacc tcggcctttg tcggctgcgc
     5641 ttgggtgccg ccgccgcgcc agccccgcgc ggcggccttc agcaccaggt ccccgctccg
     5701 ggggtaaacc cggcaggcgt ggacacccgg cgagcgaagg gtgcgcgccg gggctcgacc
     5761 ggcaaccgct gcttcccggt agcgggaaag cccgtggcga tggtaccttt tcgtgcatac
     5821 ctttgccgcg ctcaattttg tgcgccccgc aacgggctca ggcgagatgg tgcgcacacg
     5881 tataggcggc gaaaaacggt ccatattgga gaactgccgt gacggtagcg gagattcttg
     5941 cagcaaaggg ttccaaggtc atcaccatca gcccgtcgga cacgatcacg acgctatgcg
     6001 agtgtttgcg cgaaaagcgg atcggcgctg ccatcgtcag ccgcgacggc aagacggttg
     6061 agggggtgat ttccgagcgg gacgtggcct atggactggc cgtgcataag gacgagctgc
     6121 ccgacctgcc ggtcgccgcc ctcatgacca aggccgtcat cacctgttcg cccaacgatc
     6181 ccgtggccct ggtcgcctca accatgctgg cccggaatat tcgc
//
LOCUS       DASTMT010000161         1594 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_418807, whole genome shotgun sequence.
ACCESSION   DASTMT010000161 DASTMT010000000
VERSION     DASTMT010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 1594)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1594)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1594
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_418807"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1146)
                     /locus_tag="VFR00_03610"
     CDS             complement(<1..1146)
                     /locus_tag="VFR00_03610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent hydrolase"
                     /protein_id="HEU0158371.1"
                     /translation="MPSNLRIDPERLWSTLMESGAIGATAKGGINRQTLTAEDAAVRA
                     WFKREAETLGCSVSVDEVGNMFALRAGRQPGLAPIALGSHLDTQPTGGKFDGNLGVLG
                     ALEALRTLVSAGYETNAPIVIVNWTNEEGARFAPAMLGSGVFAGVYSRAAADAIADIE
                     GIRFGAALDAIGWRGESKAGMPLSAYLELHIEQGPILEAEHKTIGVVTGVQGVRWYEL
                     KLSGRESHAGTTPMPRRADALVAAARLIGAVRELALAQAPTAVGTVGQLEVEPNSPNV
                     VPGHVRMTVDLRHHDDAILEAMETALRAAVERVGGEEKIEISLSKLHDLAAIKFHPAC
                     IAAVRSAARALGYRERDIISGAGHDAVHMSRITPSAMIFVPCKDGLSH"
     gene            1347..1423
                     /locus_tag="VFR00_03615"
     tRNA            1347..1423
                     /locus_tag="VFR00_03615"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1381..1383,aa:Pro,seq:cgg)
BASE COUNT          269 a          507 c          547 g          271 t
ORIGIN      
        1 gtggctgagg ccatccttgc agggcacgaa gatcatggcg ctcggcgtga tgcgcgacat
       61 gtgtaccgca tcgtgtccgg caccggagat aatgtctcgt tcgcgatagc ccaaggcgcg
      121 ggccgcgctg cgcaccgcgg cgatgcaggc gggatgaaat ttgatcgccg ccaggtcgtg
      181 gagcttgctg agcgagatct cgatcttctc ctcgccaccc acgcgctcga ccgccgccct
      241 gagtgcggtt tccatggcct cgagaatcgc atcgtcgtga tggcgcaggt cgaccgtcat
      301 gcgcacgtgt cctggcacga cgttgggcga atttggctcg acctccagct gaccgaccgt
      361 gcccacggcc gtcggcgcct gcgctaaggc gagctcgcgc acggcgccaa tcagacgtgc
      421 tgcggccacc agggcgtcgg cgcggcgcgg catcggcgtg gtgccggcat gactttcgcg
      481 gccactgagc ttgagctcat accagcgcac gccctgaacg cccgtgacca cgccgatggt
      541 tttgtgctcg gcctcgagga taggaccctg ctcgatatgc aactccagat aggcggagag
      601 cggcatgccg gctttgctct cgccgcgcca gccgatggca tcgagcgccg ccccgaagcg
      661 gatgccctcg atatcggcaa tggcatcggc cgcagcgcgg ctatagacgc cggcaaacac
      721 ccccgagccc agcatggccg gggcgaagcg ggcaccctct tcgttggtcc agttcacgat
      781 aacgatgggt gcattggtct catagccggc gctgacgagg gtacgaaggg cctccagcgc
      841 cccgagcaca ccaaggttgc cgtcaaattt gcccccggtg ggctgggtgt cgagatggct
      901 gccgagcgcg atcggagcca agccgggctg gcggccggcg cgcagggcaa acatgttgcc
      961 gacctcgtcg acgctgacgg agcaccccaa ggtctcggcc tcgcgtttga accaggcgcg
     1021 cacggcggcg tcttcggcgg tcagggtttg tcggttgatg ccgcctttgg ccgtggcgcc
     1081 gatggccccg ctctccatca gcgttgacca gaggcgttct gggtcgatgc gcaagttgct
     1141 gggcatttgg gctgatccgg ggcgtgaacg ggagcaaggc ttgccattgt cgccgcgccc
     1201 tagcctggcg taaagaccga tcggcggcag cagccgacct cgccttcaag aaccggcagg
     1261 ggacgggctc gcttgcgaga tggtggcgcc gcggctataa ggcgctcaac gcggcggcgg
     1321 gtgccggttg ccgtcgacag gtcgggcgga gcgtagcgca gcctggtagc gcacttgctt
     1381 cgggagcaag gggtcgggag ttcgaatctc cccgctccga ccaacaaaat caaggactta
     1441 gatgaatggc ccagaccccg ctaaccggat tgtaaccgga atgacaaggg gtggttaagc
     1501 ccggactcgc gggcgcgcac gcgcggcttc ctcctgaaag tccggatggt ggtggccata
     1561 gtacctccac agagtatccg ggtgcatacc aagg
//
LOCUS       DASTMT010000162         2145 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_2714141, whole genome shotgun sequence.
ACCESSION   DASTMT010000162 DASTMT010000000
VERSION     DASTMT010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2145)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2145
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2714141"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..570
                     /locus_tag="VFR00_03620"
     CDS             <1..570
                     /locus_tag="VFR00_03620"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031966 - mitochondrial membrane
                     [Evidence IEA];
                     GO_process: GO:0033617 - mitochondrial cytochrome c
                     oxidase assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEU0158372.1"
                     /translation="LLAGGAAAGADEQLRPAPPAPLRFSDADGHLVLTSDFAGKWLLI
                     YFGYMHCSDLCPTGLSVLATALDQIGPAARNIQPLFVTVDPERDRGPELRRFAQAFDK
                     RLIGLGGSLADIKRAAAALDVSFEKVAQGDAGYVVDHSSGYVLIDPARETVRALRFAE
                     PHLLAAKLIAVMTAAGTPLEGLNNIGAYR"
     gene            complement(561..2084)
                     /locus_tag="VFR00_03625"
     CDS             complement(561..2084)
                     /locus_tag="VFR00_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008967637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0158373.1"
                     /translation="MPERQGRLWRMHGGEDMVEFSKLGAAGLPGAAGLLAALIAVAAP
                     HPLLAQGRAEAKDMALVGYDDLQARSAYQPSIHKQGERWIAYIGHHGGSKEVPKPRNP
                     LTGVDEFNGTSIVDVTDPRRPKYLFHIPGDQGLAEEGGAQMVRVCDGKSLPKGDANAV
                     YMLRTFGNQAHEIWNTADPSSPKLVARLDGLKGTHKNWWECDTGIAYLVSGVAGWRVA
                     RMTEVYDLSDPAHPVKIRDFGLPGQQPGAGGPVPTRLHGAISTGAKGNRIYFGYGTNE
                     GGVLQIVDRDKLLNGPKEPTDDNLTYPQVGRLDMSPLTGAHTTFPVLEMPVAEFAKDK
                     VGKVRNFVVVTNEQIKNECEEARQFVWMVDVTVERYPASVATWGVPEASGGFCSRGGR
                     FGTHASNESSAPVYYKRLLFFSHFNAGVRAVDIRDPYHLKEVAHYIPAVTRNTDKRCI
                     TLENGAERCKVAIQTNNVEVDERGYIYAVDRANTGMHILKLTGQARRIGNFAAAVQR"
BASE COUNT          339 a          747 c          674 g          385 t
ORIGIN      
        1 ctcctcgccg gcggcgccgc ggccggggcc gacgagcagc tgcgacccgc cccgccggcg
       61 ccgctccgct ttagcgatgc cgacggccac ctcgtcctca cgagcgattt cgccggcaag
      121 tggctcctca tctatttcgg ctatatgcac tgctccgatc tgtgtccgac cggcttgagc
      181 gtgctcgcca ctgcgctcga tcagatcggg cccgccgccc gcaacatcca gcccctgttt
      241 gtgaccgtcg atcccgagcg cgaccggggc ccggagcttc gccgctttgc gcaagccttt
      301 gacaagcgcc tgatcggcct cggcggctcg cttgccgaca tcaaacgcgc cgccgccgcg
      361 ctcgatgtgt ccttcgagaa agtggcgcag ggagacgccg gctacgtcgt cgaccacagc
      421 tcgggttatg tgctgatcga ccccgcgcgc gaaaccgtgc gcgccttgcg gttcgccgag
      481 ccgcatttgc ttgccgccaa gctgatcgcc gtcatgaccg cggccggcac gccgctcgag
      541 ggcctcaaca atatcggcgc ctatcgctga acggcggcgg cgaaattgcc gatccgccgg
      601 gcctggccgg tcaatttcag gatatgcatg ccggtgttgg cgcgatcgac ggcgtagatg
      661 tagccacgct cgtcgacctc cacgttgttg gtctggatcg ccaccttgca gcgctcggcg
      721 ccgttttcca gggtgatgca gcgcttgtcg gtattgcgcg tgaccgccgg gatgtaatgc
      781 gccacctcct tgaggtggta gggatcgcga atgtcgacgg cgcgcacgcc ggcattaaag
      841 tgcgaaaaga acagcaagcg cttgtagtag acgggcgctg agctttcgtt ggaggcatgc
      901 gtgccgaaac gcccgccgcg ggagcagaag ccgccgctcg cctcggggac gccccaggtc
      961 gccaccgagg ccggatagcg ctccaccgtg acgtcgacca tccacacgaa ctgacgcgcc
     1021 tcctcacact cgttcttgat ctgctcgttg gtaacgacca cgaagttgcg caccttgccc
     1081 accttgtcct tggcaaattc cgccaccggc atctccagca ccggaaaggt ggtatgcgcc
     1141 cccgtgagcg gcgacatatc gagccgacca acctgcggat aagtgaggtt gtcgtcggtc
     1201 ggctccttgg gaccgttgag aagcttgtcg cggtcgacga tctgcaatac cccgccctcg
     1261 ttggtgccgt agccgaagta aatgcgattg ccctttgccc ccgtggagat agcgccgtgc
     1321 agccgggtgg gcacggggcc gccggcgccg ggctgctggc ccggcagccc gaaatcgcga
     1381 attttcacgg ggtgagccgg gtcgctcaag tcataaacct cggtcatgcg cgccacccgc
     1441 cagccggcga cgcccgaaac gaggtaggcg atgcccgtgt cgcattccca ccaattcttg
     1501 tgcgtgccct tcaggccatc gagacgggca acgagcttgg ggctgctggg atcggccgta
     1561 ttccaaatct catgcgcctg attgccgaac gtgcgcagca tgtagacggc attggcatcg
     1621 cccttgggta agctcttgcc gtcgcacacc cgcaccatct gcgccccgcc ctcctcggcc
     1681 aacccctgat cgcccggaat gtgaaagaga tatttgggcc ggcgcgggtc ggtcacatcg
     1741 acgatcgacg tgccgttgaa ctcgtccacg cccgtcagcg gattgcgcgg cttcggcacc
     1801 tccttgctgc cgccgtgatg gccgatgtag gcgatccagc gctcgccctg cttatggatg
     1861 ctgggctggt aggcgctgcg cgcctgcaag tcgtcgtagc cgaccagcgc catgtccttg
     1921 gcctcggcac gcccctgcgc caggagcggg tgcggcgccg ccacggcgat gagagccgcc
     1981 agcagcccgg cggctcccgg cagcccggcg gctccaagtt tgctgaactc gaccatgtcc
     2041 tcacccccgt gcatgcgcca cagccggcct tggcgctcag gcatcggcga cccaaagaaa
     2101 ggcgccgtcc tttcgttcca cgcgtccgtg gcccggaaac ggcag
//
LOCUS       DASTMT010000163         1953 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_1075582, whole genome shotgun sequence.
ACCESSION   DASTMT010000163 DASTMT010000000
VERSION     DASTMT010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 1953)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1953)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1953
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1075582"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(304..918)
                     /locus_tag="VFR00_03630"
     CDS             complement(304..918)
                     /locus_tag="VFR00_03630"
                     /inference="COORDINATES: protein motif:HMM:TIGR00413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HEU0158374.1"
                     /translation="MRIPWRPSVVSCLALAAAAGVTAACSGLGNEARYGSASPRLVDP
                     TQPIPKGGGAYKLGAPYQIDGRWYVPQDDPRYDRSGFASFYAEDFHGRRTSNGEIFDM
                     WALTAAHPTLPLPSLAYVTNLENGRTLLVRLNDRGPYVNNRVIDLSRASARYLGVEGP
                     GLARVRVRYAGPAPLSGDDRREQRFLASQPWFQIKLARGPNDSR"
     gene            complement(1090..>1953)
                     /locus_tag="VFR00_03635"
     CDS             complement(1090..>1953)
                     /locus_tag="VFR00_03635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0158375.1"
                     /translation="KARHAHWPRALCAGAAAMAIWIGIRLELPPDAYIAGVLATAALN
                     FIDLSSLTLDPMRLLLVALAGYGLALLALSWAKLERAHFYAAVAVVSVLSVHVLTSDT
                     PIVGENRYYLRTALLTFTPILGMLAVAYAGTGEGRLQLALPSLRRLLGTLASAVSIRC
                     AAGAMLVAMSVHVLETERFVAAWISYEDAVRKLATGSLSDPALGDPRFVSANRIPDRL
                     NGLVWASTTHFLSILVAPAFAPARLVVDPGANYFWLPCRTAAANSVGRRAVPLVTRQL
                     LRKYACLHRRP"
BASE COUNT          356 a          648 c          659 g          290 t
ORIGIN      
        1 agccatgtgg aggcgcatca ttttgacccg cacgtcatgc cattgtcccg tcactcaggc
       61 ccgtcactcg ctaactccct cggctacgcc ccggcacttc aaggtcatcc cgtcgcccgg
      121 cggcaaacgg ccacccccga tacccccttc acccccgtac ggtcgagcgc cggcatggtt
      181 tcgcaccccc cgtgtctcct ccccgttcgg gagcgcctga agagttgtat gccccgccgt
      241 tgccgacgct atgtcgcccc agggcgctca gcgtgcgctc gcctggcagc atagtctcga
      301 atcctagcgg ctgtcattgg gtccgcgcgc cagcttgatt tgaaaccagg gctgggaggc
      361 gagaaagcgc tgctcccggc gatcgtcgcc cgacaagggc gccgggcccg cgtagcgcac
      421 gcgcacgcgg gctagccccg gtccctcgac gccgagatag cgcgcgctcg cccgcgacag
      481 gtcgatcacc cgattgttga cataaggtcc ccgatcattg agtcgcacca gcagcgtgcg
      541 tccattttcc agattggtga cgtaggcgag cgagggcaac ggcaaggtgg ggtgggcggc
      601 ggtcagcgcc cacatatcga agatttcgcc attggacgtg cggcgaccat gaaagtcttc
      661 ggcatagaag gaggcaaagc cgctacgatc gtagcgggga tcgtcctgcg gcacatacca
      721 gcgcccatcg atctgatagg gtgcgcccag tttgtaggcg ccgccgcctt tgggaatggg
      781 ttgcgtgggg tcgaccaggc gcggactggc agagccgtag cgggcctcgt tgccaaggcc
      841 cgagcaggca gcagtgacgc cggccgccgc ggccagcgct agacaggaga cgaccgacgg
      901 ccgccaggga atacgcatca cgatcaaacc ttggccttgg ctcccctcga ggagccgccc
      961 ggaccgttcc gggccccgca accgaaaagc ttgccaaacg gccgttagga acgggttaac
     1021 tctgcggcgc gagggcgctc agcgacttgg ctggcgcccg gcgatgcttg gcgctgccgc
     1081 gtgcggcgct cagggtcttc ggtgcaggca ggcgtacttg cgcaggagct gccgggtgac
     1141 gagcggcacg gcgcgccgcc cgacgctgtt ggccgctgcg gttcggcaag ggagccagaa
     1201 atagttggcg cccgggtcga ccaccaggcg tgccggcgca aaggcggggg cgaccaggat
     1261 ggagaggaaa tgcgtggtgg aggcccacac caggccattg agcctgtccg ggatccggtt
     1321 cgccgaaaca aagcgcggat cgccaagggc cggatcggaa aggctgccgg tcgccagctt
     1381 gcgtacggcg tcctcgtaac tgatccaggc ggcgacgaac ctttccgtct cgagcacgtg
     1441 cacggacatg gccaccagca ttgcgcccgc cgcacagcgg atgctgacgg cactggcgag
     1501 ggtgccgagc agacgccgca gagacggcag cgccagctgt agccgacctt cgccggtccc
     1561 ggcgtaggcc accgccagca tccccaagat gggcgtaaag gtcaaaagtg ccgtgcgcag
     1621 ataatagcgg ttttcaccga cgatcggcgt gtcggacgtg agcacatgca cgctcagcac
     1681 cgagaccacc gccacggccg catagaaatg cgcgcgctcc agtttggccc agctcaatgc
     1741 cagcagcgcc agaccgtagc cggcgagcgc gaccaagagc agccgcatgg gatcgagagt
     1801 gaggctactg agatcgatga aattgagcgc cgccgtagcc aacacgccgg cgatgtaggc
     1861 gtcgggtggc aactcgaggc gaatgccgat ccagatcgcc atggccgcgg cgccggcgca
     1921 gagcgcgcgc ggccaatgcg catggcgcgc ctt
//
LOCUS       DASTMT010000164         2693 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_2715356, whole genome shotgun sequence.
ACCESSION   DASTMT010000164 DASTMT010000000
VERSION     DASTMT010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2693)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2693)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2693
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2715356"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1333
                     /locus_tag="VFR00_03640"
     CDS             <1..1333
                     /locus_tag="VFR00_03640"
                     /inference="COORDINATES: protein motif:HMM:NF013086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEU0158376.1"
                     /translation="ETFDGRRLGSAFGKLRAPIKSMMAFGGMMIGRNDLPHIFRMSRS
                     LKAASHVGRMVARYGRDRLTHQRGTRLVNGNALIARMAAQAFARGIPLWLSSPIVELS
                     REHGRIIGAVVEKEGRRQSVSTRLGVVLACGGFPGDDELTRGLYPHRRAGKNHATLPP
                     PGNAGDGLRLAQSVGGRLNDAVHQPAAWAPVSLVPQPDGSLIPFPHFYERGKPGYISV
                     DRRGRRFVNESKSYHVYIPELIEACRDDPVIEAWIVCDHAAIRRFGLGALGPAPMPMR
                     AFLASGYIKRADSAAALALACGIDAPGLDQTLRVFNSAARGGTDPEFQRGLDAYQRFN
                     GAADHLPNPCVAPLQKPPFYAVRVVPGELGTFAGIATNAHGQVIDADGVALDGLYAVG
                     NDAASVMGGTYPGAGITIGPAMTFGYLAARHLAAARRGPAVQRARAQSAQP"
     gene            complement(1324..1581)
                     /gene="grxC"
                     /locus_tag="VFR00_03645"
     CDS             complement(1324..1581)
                     /gene="grxC"
                     /locus_tag="VFR00_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012334927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HEU0158377.1"
                     /translation="MPRVVLYTTRFCPYCLMAKRLLGEKGAAFEEIDVGGNPALRREM
                     TLKANGRRTVPQIWIGETHVGGCDELYELEAKGDLDRLLQG"
     gene            1933..>2693
                     /locus_tag="VFR00_03650"
     CDS             1933..>2693
                     /locus_tag="VFR00_03650"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HEU0158378.1"
                     /translation="MKAPKFSYVRATSLGQVLELLERYGEDAQILAGGQSLMPALNMR
                     LSAPRLLVDINGIAALQGITLEEGVVRIGALARHAEVARSAMVRQHLPLIAAAMPHVA
                     HLAVRNRGTFGGSIALADPAAELPACAVALDACFVLQSLAGRREVAASDFFKGFYATA
                     RRPNELLIEALIPVRQNWVPVFLELARRHGDFAIAGVAFQVLLAEGIVADARLAYFAS
                     EARPTLGFATLAAIKGQRLDQTTTEAAVSALPKDLA"
BASE COUNT          406 a          935 c          890 g          462 t
ORIGIN      
        1 cgagacattc gacgggcgcc ggcttggcag cgcatttggc aagctgcggg cgcccatcaa
       61 gagcatgatg gcgttcggcg ggatgatgat cggtcgcaac gatctgccgc acatttttcg
      121 catgtcgcgc tcgctcaagg ccgccagcca tgtcgggcga atggtggcgc gctacggtcg
      181 cgatcggctg acacaccagc gcggcacgcg gctcgtgaat ggcaacgcct tgattgcccg
      241 gatggccgcg caagccttcg cccgcggcat accgctctgg ttgtcctcac cgatcgtcga
      301 gctcagccga gagcacgggc gtattatcgg ggccgtggtg gagaaggagg gtaggcgcca
      361 gagcgtatcg acccggctgg gcgtcgtctt ggcctgcggc ggctttcccg gcgacgacga
      421 gctcacccga ggcctctacc cccaccgccg cgccggcaag aaccatgcga ccttgccgcc
      481 gcccggcaac gcgggcgacg gcctgcgctt ggcacaatcc gtaggcggcc ggctcaatga
      541 tgccgtgcac cagcccgccg cctgggcgcc cgtgtcgctg gtgccgcagc ccgacggcag
      601 ccttattccg ttcccgcatt tctacgagcg cggcaaaccc ggctatatca gcgtcgaccg
      661 gcgtggccgg cgcttcgtca acgagtccaa gtcctatcac gtctacatcc ccgagctgat
      721 cgaggcctgc cgcgatgatc ccgtgatcga ggcatggatc gtgtgtgacc atgccgccat
      781 tcgacggttt gggctcggcg cgctcggacc ggcgccgatg ccgatgcggg cattccttgc
      841 gtcgggttac atcaagcgcg ccgatagcgc agctgccctg gcgcttgcct gcggcatcga
      901 tgcgccggga ctagatcaga cgctgcgcgt cttcaacagc gccgcgcgcg gcggcacgga
      961 tccggagttt caacgcggct tggatgctta ccagcgcttc aacggcgcgg ccgaccacct
     1021 tcccaatccc tgcgtcgcgc cgctgcagaa acccccgttc tatgccgttc gcgtggttcc
     1081 gggcgaactt ggcaccttcg cgggtatcgc caccaacgcg catgggcaag tgatcgatgc
     1141 cgacggcgtt gcgctcgatg gcctctatgc cgtcggcaat gacgcggcca gcgtgatggg
     1201 cggcacctac cccggcgcgg gcatcaccat cggtcccgcc atgacatttg gctatctcgc
     1261 cgcccggcat ctggcggccg cacgccgggg gcccgcagtg caacgggcgc gtgcgcaatc
     1321 ggctcagcct tgaagcagcc ggtcgaggtc gcccttcgcc tcgagttcat aaagctcatc
     1381 gcagccgccg acgtgggtct ccccgatcca gatctgcggc acggtgcggc gcccgttggc
     1441 cttcaacgtc atctcccggc gcagcgcggg attaccgcca acgtcgattt cttcgaaagc
     1501 agcgcctttc tcgcccagta ggcgcttggc catcaggcag tacgggcaaa agcgcgtggt
     1561 atagaggaca acccttggca ttctgacccc gtgtgaaggc gcgaacacgc cgcctttgct
     1621 agcgcgcgcc gcgacggctt gccaggggaa cgccgcaggc gcccacgggt tgtcgcgctc
     1681 gaccgatttg ttttgcgcat tcgcaacggc tcggtcaacg ggcacaaggg gaccccagcg
     1741 ccgacctcgt cctgggcggt ttctgcaggc ctttgaaggc ggtgaggaca atcgccgcgc
     1801 cgatgcccgg gagcctccca tgccgccgct gaggcccagg caggggcggg ggcgccccgt
     1861 ccgcttgtcg cttgcgccac gccgagcgca atggcagcct ccggcccaga gcaggtttgc
     1921 cgcgagccgg gtgtgaaggc gccgaaattc agctacgtcc gcgccaccag cctcgggcag
     1981 gttctggagc tgctcgagcg ctatggcgaa gatgcgcaaa tcctcgccgg cgggcagagc
     2041 ctgatgccgg ccctcaacat gcggctgtcc gcgccccggc ttcttgtcga tatcaatggg
     2101 atcgcggcgc tgcagggcat caccttagag gagggcgtgg tgcgcatcgg tgcgctggcg
     2161 cgacatgccg aggtcgccag gtccgccatg gtccgccaac acctgccgct gatcgctgcg
     2221 gccatgccgc acgtggccca cctcgccgtg cgtaaccgcg gcacctttgg gggcagcatc
     2281 gcgctggccg atccagccgc ggagctgccg gcctgcgcgg tggcgctcga cgcatgcttc
     2341 gtgctgcaaa gcctcgccgg ccggcgcgag gttgccgcct cggatttttt caagggtttc
     2401 tatgcgacgg ctcgccggcc caacgagctg ctgatcgagg cgctgatccc ggtgcgacaa
     2461 aactgggttc ccgtctttct ggagctggcg cgccgtcacg gcgattttgc catcgccggg
     2521 gtggcctttc aggtgctgct cgcagagggc atcgtcgccg acgcccgctt ggcctatttt
     2581 gccagcgagg cgcggcccac gctcggcttc gccacgctgg cggcaatcaa gggccagcgc
     2641 ctcgatcaaa ccaccaccga ggccgcggtc agcgccctgc cgaaagatct ggc
//
LOCUS       DASTMT010000165         2318 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_2060700, whole genome shotgun sequence.
ACCESSION   DASTMT010000165 DASTMT010000000
VERSION     DASTMT010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2318)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2318)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2318
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2060700"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..206)
                     /locus_tag="VFR00_03655"
     CDS             complement(<1..206)
                     /locus_tag="VFR00_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008195103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HEU0158379.1"
                     /translation="MDDRRDEEQNRLTARAARYVRVGTNVGAVAARVAGQRLLGLDAD
                     SSKNAAALAAALGGLKGPIMKVAQL"
     gene            330..1586
                     /locus_tag="VFR00_03660"
     CDS             330..1586
                     /locus_tag="VFR00_03660"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0158380.1"
                     /translation="MAPSVSSGAEVGAGAWLRQPATHAILALGITQIIAWGTTLYALG
                     VLGKPIAEDTGWSQSLVFGGLTIGLLVSAAVSTLVGRGIDRRGGRAVMGLGSLLMAAG
                     LVLLALVESPAAYLLAWAFLGLAMRMCLYDAAFAALVQVTPSRGRRAISYLTLFGGFA
                     SSVFWPIAHLINAAYGWRMTLLIFAAINLLVCMPLNWLGLARRETPTQADVVDMATPA
                     SASGPPPLQGPARTMAMLLFGAIVAASAIVFGAMAAHLVPVLEASGLAAATAVWIASL
                     KGVAQVAGRVWDLTLARKWHPIDVGRVSIAIMPLSFMVLLLGGAHFLAALTFTLLFGI
                     SNGLVTIMRGAVPLALFGTHGYGEVLGILATPYLVLAALAPAAFALVVEAYGYGLAQA
                     VMLGAGLCSLLGMEIMSFWYRRRQRH"
     gene            complement(1617..>2318)
                     /locus_tag="VFR00_03665"
     CDS             complement(1617..>2318)
                     /locus_tag="VFR00_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HEU0158381.1"
                     /translation="WNWPVWKAAILMVPLLLIEQAFLAAQVLKIPDGGWLPLSMAGTI
                     CLIVYTWVRGSASLSRATRKGEADLEWLVRKLDAKPPHRVPGTAVFLTGDPSAAPTSL
                     MHNLKHNRVLHERNIILTIKTEDTPRVPRHERIEIDRVADTFIRVIAHYGFMETPSVP
                     KIIDHCRRKDLNIDISATSFFLSRRSLKTTMKSEMPSWQERFFIWLAGRAEDATEYFR
                     IPSDRVVEVGTQVMV"
BASE COUNT          341 a          741 c          762 g          474 t
ORIGIN      
        1 agctgcgcca ccttcatgat cggacccttg aggccgccca gggctgccgc gagggcagcc
       61 gcatttttgc tgctgtcggc gtcaagcccg agcagccgct gaccggccac ccgggcggca
      121 acggcaccga cgttggtgcc aacgcgcacg tagcgggcgg cgcgcgccgt tagccggttc
      181 tgctcctcgt cgcgtcgatc gtccaagggc tagtactcct cgcccctcaa atatcggcgc
      241 gcacctgccc tcagtccagg gctgatgcgt ggcacgcaat gccgcgctaa gctgggcggg
      301 aattcacagc ggcaaaggtg cgatcgggca tggccccatc cgtctcctcg ggcgccgagg
      361 tcggcgccgg cgcctggctc agacagccgg caacgcacgc tattctggcg ctcggcatta
      421 cgcaaatcat cgcctggggt accacgcttt atgccctcgg tgtgctcggc aagccgattg
      481 ccgaggatac cggctggagc caaagcctgg tgttcggcgg cttgacaatc ggcctcttgg
      541 tctccgccgc ggtctcaacg ctcgtcgggc ggggcattga tcggcgcggc gggcgtgcgg
      601 ttatgggtct tggctcgttg ctgatggcgg cgggcttggt gctgctggcg ttggttgagt
      661 cccccgccgc ctatctgctc gcctgggctt ttttaggcct tgccatgcgc atgtgccttt
      721 acgacgcggc ctttgccgcc ctcgtgcaag ttaccccgtc gcgtggccgg cgcgcgattt
      781 cctatctcac gctgtttggc ggttttgcct cctccgtatt ctggccgatc gcgcacctca
      841 tcaatgctgc ctacggttgg cggatgacgc tgctgatatt cgcggccatc aacttactcg
      901 tttgcatgcc gctgaactgg ctgggtctcg ccaggcgcga aaccccaacg caggccgacg
      961 tcgtcgatat ggccacgccg gcttccgcga gcggcccgcc gccgctgcaa ggcccggcgc
     1021 ggaccatggc catgctcttg ttcggtgcga tcgtggcagc aagcgccatc gtattcggcg
     1081 ccatggcggc gcatttggtg ccggtcttgg aggcgtccgg tcttgccgcg gcgaccgcgg
     1141 tttggatcgc ctcgctcaag ggcgtggcgc aggtcgccgg ccgagtctgg gatctgacgc
     1201 tggcacgcaa gtggcacccg atcgatgtcg ggcgcgtctc catcgccatc atgcccctct
     1261 cgttcatggt cttgctgctg ggcggcgccc actttctggc ggcgctaacg ttcaccttgc
     1321 tgttcggcat ctccaatggg ttggtgacga tcatgcgcgg cgccgtgccg ctcgccttgt
     1381 tcggcaccca cggctacggc gaggtccttg gcattctggc gacgccgtac ctcgtcctcg
     1441 ccgccctcgc cccagctgcg ttcgcgctcg tcgtcgaagc ctacggctac gggctggcgc
     1501 aggcggtcat gttgggcgcc ggcctgtgct cgctgttggg gatggagatc atgtcgtttt
     1561 ggtaccgccg gcggcagagg cattaatcct tgccggcagg tggcggcgtg ggcggcctag
     1621 accatcacct gggtgccaac ttccaccacg cgatcgctcg gaatgcggaa gtactcggtg
     1681 gcatcctcgg cgcggcccgc cagccagata aagaaacgtt cctgccagct tggcatttcc
     1741 gacttcattg tggtcttgag cgagcggcgg gacagaaaaa aggatgtcgc ggaaatatcg
     1801 atgttgaggt ctttgcggcg acagtgatcg ataatcttcg ggacgctcgg ggtctccatg
     1861 aacccgtaat gtgcgatcac gcgaatgaag gtatcggcga cgcgatcgat ctcgatgcgt
     1921 tcgtgccgcg gcacgcgcgg ggtatcctcg gtcttgatcg tgagaataat gttgcgctcg
     1981 tgcaggaccc ggttgtgttt gagattgtgc ataagcgacg ttggcgccgc gctcggatcg
     2041 ccggtcagaa acacggccgt cccaggcact cggtgcggcg gtttggcgtc aagcttgcgc
     2101 accaaccatt cgagatcggc ttcgcctttg cgggtggcac gcgacagcga ggcggagccg
     2161 cgcacccaag tatagacgat gaggcagatg gtgcccgcca ttgacagcgg cagccagcca
     2221 ccgtcgggaa ttttgagcac ctgcgccgcc aagaaggcct gttcgatcag gagcagcggc
     2281 accatgagaa tggcggcctt ccacacgggc cagttcca
//
LOCUS       DASTMT010000166         3993 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_3045348, whole genome shotgun sequence.
ACCESSION   DASTMT010000166 DASTMT010000000
VERSION     DASTMT010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3993)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3993)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3993
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3045348"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2356)
                     /locus_tag="VFR00_03670"
     CDS             complement(<1..2356)
                     /locus_tag="VFR00_03670"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEU0158382.1"
                     /translation="MSARPGMHCPRCGLSSPTGKFCSECGTPLEAKSVGQAAAQATRK
                     IATVLFADIKGSTKAISAIDAEGAMLRIRPMINAMAAAVREQGGTVIEVRGDGILSGF
                     GALDSLEDHPLAACRAALAIRQAAKGGASPGLPVRIGIHTGEVIVTPGEAGNSMMGAT
                     VHLASRLETAAEPNTILISEDVYRAVAPAAEVQPRGRFTFKGFDQPVETWELLDVEHR
                     SRWGARALLGLTPYCGRASERAQLKFFIEQAKQGSGGVLSITGDPGAGKSRFIHDALT
                     SGLVDDCTIWFAESELPARHSPYAIARSIARSWLGLTDHNTAQDVERRLSAILQQFAE
                     TILDHAPALKALLNLSVADTDWAHFDQMERKRRLAACFLEISRLQARHRPLFIIVDDA
                     QWCDPETLQLLAEVARHVPSMSAGLILLRRAGDDAELDQDFPAAATIRLRELSAEETQ
                     GFLSVVMGSDASLEPVKAKLTEMAGGLPFFLEEAVRYLTRNGILVGEAGDYRVLAPAG
                     AINIPDTVEALVSSRISSLPAELRSVLSAASIVGRTGPLALIARICDSSVADVQPKLE
                     NLERQHIIAISELKSETIFEFRHEFFRQVAYAMTLSSDRRVLHAKTLRAAEVLFDNRI
                     SDWITFLAYHAALASLHADAARYCRIAAEHAVLASSYSAASEFCEQAVKHLDQLPQTV
                     ENIQASIDVRLLLRVAAGGTSDFQSWLHHLTKSIELAEQIGDMSRRLLALIHRTWALN
                     FSSSAVDAVRAGEAAVGLAQALRIPSSEALARFALGQAHYVNGGY"
     gene            2725..3597
                     /locus_tag="VFR00_03675"
     CDS             2725..3597
                     /locus_tag="VFR00_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013418238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU0158383.1"
                     /translation="MATRYASVFRPGLFNDQVVIVTGGGSGIGRCTAHELASLGAKVA
                     LIGRNPEKLAAVREEIAAAGGEADRWVCDIREENTVKATIAAVLARFSRLDGLVNNAG
                     GQFAAPLAQMTQKGWDAVVRNNLTGGFLMAREAYTQWMSRHGGAIVNIVADVWQGTPL
                     MAHSGAARAGMINLTKTAAFEWSGSNVRVNAVAPGWIASSGLDRYPPEMGEKIRSLKN
                     HVPAGRLGTESEVAAAIVFLLSQAAAFISGATLCVDGAAPAAPRSLPLPPREVPMPAF
                     DGFHLTSTPMVLRD"
BASE COUNT          719 a         1220 c         1365 g          689 t
ORIGIN      
        1 ggtagccgcc attgacatag tgcgcttgtc ccaaggcaaa gcgggccagc gcctcgctcg
       61 agggtatgcg caaggcctgg gcgagcccga ccgcggcctc cccggcccgc acggcatcga
      121 cggcggaact tgagaaattg agcgcccagg tgcggtgaat gagggcgagc aggcgccgcg
      181 acatgtcccc gatctgctcg gcgagctcga tcgattttgt caggtggtgc aaccagctct
      241 gaaaatccga ggtgccgccg gcggccaccc gcaacagcag gcgcacgtcg atcgaggctt
      301 ggatgttttc gaccgtttgc ggcaactgat ccaaatgctt gacggcctgc tcgcaaaact
      361 ccgaagccgc gctgtaggag gaggcgagca ccgcgtgttc ggcggcgatg cgacagtagc
      421 gggcggcgtc ggcgtgcagg ctcgccagcg cggcgtggta ggcgaggaat gtgatccaat
      481 ccgaaatgcg gttgtcgaac aggacttcgg cggcgcgcaa ggtcttggcg tgcagcacgc
      541 gccggtcgct gctcaaggtc atggcatagg cgacctggcg gaagaactcg tggcgaaact
      601 caaagatggt ctcggacttg agttccgaga tggcgatgat gtgttggcgc tcgagatttt
      661 ccagcttcgg ctgcacgtcg gcgaccgaac tgtcacaaat gcgcgcgatc aaggccagcg
      721 gtccggtgcg tccgacaatg gaggcggccg acagcaccga gcgaagttct gcaggcaggc
      781 tcgaaatccg ggaggagacg agagcctcca ccgtgtcagg aatattgatg gcaccggctg
      841 gcgcaagcac ccgatagtcg cccgcttcgc cgaccaggat gccgttgcgg gtgagatagc
      901 ggaccgcttc ttccaggaag aagggcaagc cgccggccat ttccgtcagc ttggccttga
      961 ccggctcgag cgaggcatcc gaccccatca ccacgctcaa aaagccttgg gtctcctcgg
     1021 cactcagctc gcgcaagcgt atcgtggcgg cggcggggaa gtcctggtcg agctcggcgt
     1081 cgtcgccagc gcgccgcaac aggattaatc ccgccgacat gctggggacg tggcgggcga
     1141 cctcggccag aagctgcagg gtttccgggt cgcaccattg cgcatcatcg acgattataa
     1201 agagcggccg atgacgcgcc tgcagccggg aaatttccag aaagcaggcg gccagccgac
     1261 gcttgcgctc catctgatcg aagtgcgccc agtcggtgtc ggcgacactg aggttgagca
     1321 gcgccttgag ggcgggggca tggtcgagga tggtctctgc aaattgttgc agaattgccg
     1381 aaagccggcg ctccacatcc tgcgccgtgt tgtggtccgt caggccgagc cagctgcgcg
     1441 cgatcgagcg ggcgatggcg tagggcgaat gccgcgccgg cagctcgctt tcggcgaacc
     1501 agatcgtgca gtcatcgacg agacccgagg tcagcgcgtc gtgaataaaa cgcgactttc
     1561 cggcaccggg atcaccggtg atgctgagga cgccgccgct gccctgtttg gcttgctcga
     1621 tgaaaaactt gagctgggcg cgctcggacg cgcggccaca ataaggtgtc aaccccaaca
     1681 gggcgcgcgc accccagcgc gagcggtgtt ccacgtcgag cagttcccag gtttcgacgg
     1741 gctggtcgaa gcccttgaac gtgaagcggc cccggggttg cacctcggcg gcgggggcga
     1801 cggcgcgata gacgtcttcg gagatcagga tggtattggg ttcggctgcc gtctcgagcc
     1861 ggctggcgag atgaaccgtg gcacccatca tggaattgcc ggcctcgccg ggggtgacga
     1921 tgacctcgcc ggtgtgaatg ccgatccgca ccggcagccc cggcgaggcg ccgcccttgg
     1981 cggcctggcg aatggcaagc gcggcccggc aggcggccag cggatggtct tcgagactat
     2041 ccaaggcgcc aaagcctgag aggatgccgt cgcctctgac ctcaatgacg gtgccgccct
     2101 gctcccgcac ggccgccgcc atggcgttga tcatgggcct gatgcgcagc atggcaccct
     2161 cagcgtcaat cgcgctgatg gccttggtcg accccttgat gtcagcaaaa aggacggtcg
     2221 caatcttacg cgtggcctgc gcagccgcct ggccaacgct cttggcctcc aagggcgtgc
     2281 cacactcgct acaaaatttg cccgtcggac tggatagtcc gcagcgcggg cagtgcatcc
     2341 ccggtcgtgc cgacattctt gccgttaccg cgttacacgg gtttggtttt ttggccgaac
     2401 cttaagccca agtctaaagg gtcatgcggg cgccaactcc aagggctatt tgaagacgag
     2461 gtaaaatcgg ctttcctctt atagacccaa caccgggccc agatgtgctg cagatcaccg
     2521 gccacacaaa gttttgaggc gagatgccgc cgttgcgttc actcggatcg gtgcgtccgg
     2581 aagatgaccg acggcgaggc tctcatttgc cgccccgcca ccgagggcaa gaggtagctt
     2641 gatgccggga ttggcacaag ggatccgggt taacgtcgct cgacggcccg agagcccgtg
     2701 atccgggtaa atgcggaggg tctgatggca acgcgttacg cttcggtttt ccgcccgggc
     2761 ctgttcaacg accaggtcgt gatcgttacc ggcggcggta gcggcattgg ccgctgcacg
     2821 gcccatgaac tggcctccct gggtgccaaa gtggccctga tcggtcgcaa ccccgagaag
     2881 ctcgccgccg tgcgcgagga gatcgccgcc gccgggggcg aggcggaccg ctgggtttgc
     2941 gacatccgcg aggaaaacac ggttaaggcg acgatcgcgg ccgtgctggc gcggttttct
     3001 cggcttgatg gtctcgtcaa caatgcggga gggcaattcg cggcgccgct ggcccagatg
     3061 acgcaaaagg gctgggacgc ggtcgtgcgc aacaacctga ccggcggctt cctgatggcg
     3121 cgggaggcct atacccagtg gatgtcccga catggcgggg ccatcgtcaa tattgttgcc
     3181 gatgtctggc aggggacacc cctgatggcg cattcggggg ccgccagggc cggcatgatc
     3241 aacctcacca agacggcggc cttcgaatgg tcgggctcca atgtgcgggt caacgccgtg
     3301 gcgccggggt ggatcgcctc ctccggcctc gaccgctatc cgccggagat gggcgagaag
     3361 attagaagcc taaagaacca tgtgccagcc ggacgcctgg gtaccgagag cgaggtcgcc
     3421 gcggccattg tctttctctt gagccaagcc gcagccttca tatctggagc aaccttgtgc
     3481 gtcgacggcg cggcccccgc cgcgccacgc agcctaccgc tgccgccccg ggaggtgccg
     3541 atgcccgcct tcgatggctt tcatctgacg tcgacgccga tggtcctgcg cgattgaaat
     3601 ggttcgcggc cgcgccaggc ggagaacgtg gcgcttgcaa gctttgagcg aatggcggaa
     3661 gctcgtggcc gtcttcgatc gcctagcgat gtggcgatca gggagggacg tttagctcac
     3721 ttttcgcgcg acgccggtga cagcgaccct gtcggggtct tggggcctgc ctccgatcag
     3781 aggccaggca atgtcattgg tccgaggcgc tggcgcaatg cgggatggcc gagggaggct
     3841 gaaggtggtt gaagcagtcg acaagaaatc gccggtgacc gtccaccgac ggcgttttct
     3901 caaggccgcg acggcggcgg cggggagcac attgctgccc gaggtagggt ggggccagac
     3961 gctggctgca ccgccacgat cctacgattt gcg
//
LOCUS       DASTMT010000167         9024 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_2062605, whole genome shotgun sequence.
ACCESSION   DASTMT010000167 DASTMT010000000
VERSION     DASTMT010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 9024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9024
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2062605"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            116..1216
                     /gene="hppD"
                     /locus_tag="VFR00_03680"
     CDS             116..1216
                     /gene="hppD"
                     /locus_tag="VFR00_03680"
                     /EC_number="1.13.11.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008836695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003868 - 4-hydroxyphenylpyruvate
                     dioxygenase activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylpyruvate dioxygenase"
                     /protein_id="HEU0158384.1"
                     /translation="MGPFPHDAPPASISAANPAGTDGFEFVEFAHPRPLELERLFQQM
                     GYAEVARHKHKNISLYRQGSINYLVNREPNTHAARFVAEHGPSAPAMGWRVVNAQHAL
                     ERVLACGGSRYEGADTSLEVPAVIGIGGSLLYLVEAPAEGSPYDRDFHWLEARDPKPP
                     GVGFHYIDHLTHNVRRGNMDTWYRFYAETFKFREIRFFNIEGKLTGLSSRALTSPCGK
                     IRIPINESADDKSQIEEYLKRYKGEGIQHIAVGCDDIYAATDAIAARGLKFMPGPPAV
                     YYERSQARVKGHGEPIARLQKHGILIDGEGVVDAGATRVLLQIFSKTVIGPIFFEFIQ
                     RKGDEGFGEGNFKALFESIEEDQIRRGVLKAS"
     gene            complement(1304..2287)
                     /locus_tag="VFR00_03685"
     CDS             complement(1304..2287)
                     /locus_tag="VFR00_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012331194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HEU0158385.1"
                     /translation="MYALYVLGLRVFLGLGAALAAIAGVEAREPHWPPTLSIGTASPG
                     GTYFVYGEALARILTRELKTTVWARPTEGPSENIKLIETGEIDLGFVTLGVAQQAWNG
                     SGDWTNGVRYRQLRAIFPMYDTPFQFLVPRDSAILGAADLTDKRVGMGPSGGTTGIYM
                     PQFFKVLQVDARLQTGNWAELATRLVDGSLDALAVAAGAPFPAFAELERKIKVRYLPL
                     TPQQIVTLRLAMPELGPSLVAAGTYPSLLRHYSTVGLFNFAVARTALPSDLVYAIVDA
                     VFSCHEELLSYHAAAAETVPGNFTRNSFLPFHAGAAEWYNRKASSGVVRGD"
     gene            complement(2330..3073)
                     /gene="phbB"
                     /locus_tag="VFR00_03690"
     CDS             complement(2330..3073)
                     /gene="phbB"
                     /locus_tag="VFR00_03690"
                     /EC_number="1.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003296527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018454 - acetoacetyl-CoA reductase
                     activity [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetyl-CoA reductase"
                     /protein_id="HEU0158386.1"
                     /translation="MQKRVAVITGGMGGLGETISTKMADAGYRVVVTYSPSNKTSDKW
                     LAAMQQKGYAFSAYACDVTDFESSKACVGRVAAEVGPVDILVNNAGITKDMTFRKMGK
                     TDWDAVIKTNLDSVFNMTKQVYDGMMDRGWGRIINVSSVNGSKGAFGQTNYAAAKAGM
                     HGFTKSLALESARKGVTVNTISPGYIGTKMVTAIPNDVLQAKILPQIPISRLGKPEEV
                     AGLIIYLCSEEAAFVTGANIAINGGQHMQ"
     gene            complement(3102..4451)
                     /locus_tag="VFR00_03695"
     CDS             complement(3102..4451)
                     /locus_tag="VFR00_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012333328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006835 - dicarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HEU0158387.1"
                     /translation="MQAHAIPDPPKHKPFFKSLYFQVLVAIFAGVLLGHFQPHLAEQM
                     KPLGDAFIKMIKMIIAPIIFCTVVHGIASMQDMRKVGRVGLKALVYFEVVTTLALIIG
                     LLVANTLQPGGGMNVDPTSIDTRAIQGYVAKSKEQSTLQFLMDIIPNTVVGAFAQGEI
                     LQVLFFAILFAFGLQALGQRGQALLGVIDQASHAFFGVVGIIMKVAPLGAFGAMAFTI
                     GKYGLSTLVSLAHLMIAFYLTCLIFICGVLGFFAMLAGANIFKFIRYIKEELLIVLGT
                     SSSESVLPRMIAKMENLGCEKSVVGLVIPTGYSFNLDGTCIYLTMAALFLAQATNTDL
                     SLAQQLGIIGILLLTSKGAAGVTGSGFIVLAATLSSVGTIPVAGIALILGVDRFMSEA
                     RALTNLIGNGVATLVVSRWEGALDNDRMRRLLDSETDADAEEPERVMDVLSPPTPVR"
     gene            complement(4476..6752)
                     /locus_tag="VFR00_03700"
     CDS             complement(4476..6752)
                     /locus_tag="VFR00_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011086507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HEU0158388.1"
                     /translation="MDEKLLREAALDYHRAPVAGKIAISPTKGLTNQHDLSLAYSPGV
                     AYACLAIRDDPQQAALLTSRANLVAVVSNGTAVLGLGNIGPLAGKPVMEGKACLFKKF
                     AGIDVFDIEIDATEPDHIVAVVAALEPTFGGINLEDIKAPECFIVERKLRERMKIPVF
                     HDDQHGTAIIVSAAVLNGLKLVGKDITAIKVAVSGAGAAALACLDLLTALGLKRENVF
                     LSDIHGVVFKGRAAEMDSEKIRYAQDTKARTLAEILPGADLFLGLSAGGVLRAEMIVP
                     MAKDPLILALANPEPEIRPEAALAARPDAILCTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATGINEAMKIAAVKAIAELAQAEASDIVASAYGGQSLTFGRHYLIPRPFDPRLIERI
                     APAVARAAMDSGVASRPLASLEAYRQQLARFVYHSGNSMKPVFEAAKLAPKRVIFAEG
                     EEERVLRAVQVVVDEGLARPVLVGRADVIAKRIDALGLRLKLGAGCEVVNILNDPRYP
                     QVVAEYYQLGRRKGVTEAIAKEAMRSRASLIAAMLLRRGDVDAMLCGTVGTFDEHLGY
                     IEDVIGRRPGVRTLAAMNMLMLPNRQIFICDTYVNHQPRPADIAEMTLLAAEEVRRFN
                     LTPIVALLSHSSFGSSAAPEAQLMREARALLVERAPDLLVEGEMRGDAALSKVVLDDV
                     FPDSRLGSDANLLIMPSVDAANIAYNLLKIAAGSGITVGPILLGTAKPAHILEPAVSV
                     RRIVNMTAIAAVDAHVQR"
     gene            complement(7320..8321)
                     /locus_tag="VFR00_03705"
     CDS             complement(7320..8321)
                     /locus_tag="VFR00_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007599355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HEU0158389.1"
                     /translation="MARSNRQIRLVQVPKDKLALEHFALTEAPIPSPADGEVGLKVRY
                     ISLDAANRAWMQGATYRDAVEAGNIMAGAAVAEVVESKAQGFAPGDLVFADTGWQEYA
                     ALPAKRLILLPSVEPITHLLSVYGIAGLTAYFGLLRVAGPRAGETVAVSAAAGSVGSL
                     VGQIAKIKGCRVVGIAGGEDKCRWLLQELGFDAAVDYKAGPVFKALKAAAPSGLDVYF
                     DNVGGDILEACLAQMNIGGRIACCGAISAYDGPAPAHGPRGVPGLIVVKRLLLKGFIV
                     TDYYAERAAAIAELGEWVAAKKLKVQEDVIEGLENTPAALIGLLGGQNRGKRMVKVS"
     gene            8549..>9024
                     /locus_tag="VFR00_03710"
     CDS             8549..>9024
                     /locus_tag="VFR00_03710"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU0158390.1"
                     /translation="MARTFALSRRRFLASGGALVASGLSGAAAGPDAAAPAPASFRLA
                     LGAHELIVLSDGHLVVPTAMLARNVGGSELAAYLASVGFGRERVHFHINVALIRTGDE
                     HILIDAGSGGTWEPTAGRLFDSLEAAGIAPAAIGKVVITHAHPDHLWGLIDELDNSL"
BASE COUNT         1594 a         2835 c         2994 g         1601 t
ORIGIN      
        1 tcaggcttca ttgcataaat catgcaaagt aaggccagaa tgcccgtgtt tcgcaaggat
       61 cttgcgcccc attgccgcta ggttggtgca tcctgaggca caccggagac cgcccatggg
      121 tccgttcccc cacgacgccc cgccggccag catcagtgcg gccaatcccg cgggcaccga
      181 cggtttcgag ttcgtcgagt tcgcgcatcc gcggccgctc gaacttgagc ggctcttcca
      241 gcagatgggc tatgccgagg tcgcccgcca caagcacaag aacatttcgc tctaccggca
      301 gggcagcatc aattatctcg tcaaccggga gcccaacacg catgcggccc gcttcgtggc
      361 cgagcacggg ccctcggccc ccgccatggg ttggcgggtg gtcaacgcgc agcacgcgct
      421 cgagcgcgtg ctcgcctgcg gcggcagccg ctatgaggga gccgatacct cgctcgaggt
      481 gccggcggtg atcggcatcg gcggctcgct gctctacctg gtcgaggcgc ccgccgaggg
      541 gtcgccctac gatcgggact ttcattggct cgaggcgcgc gatcccaagc cgccaggcgt
      601 cggttttcat tatatcgatc acctgacgca caacgtcagg cgcggcaaca tggatacctg
      661 gtaccggttc tacgcggaga ccttcaagtt ccgcgaaatc cgctttttca acatcgaggg
      721 caagctgacg ggcctcagca gtcgggcgct gacatcgccg tgcggcaaga tccgcatccc
      781 catcaacgaa agcgccgacg ataaaagcca gatcgaggag tatctcaagc gttacaaggg
      841 cgagggcatc cagcacatcg ccgtgggctg cgacgacatc tacgccgcca ccgatgcgat
      901 tgccgcccgc ggcctcaaat tcatgccggg gccacccgcc gtctactatg agcgctcgca
      961 ggcgcgcgtg aaggggcacg gcgagccgat cgcacgcctg caaaaacatg gcatcctgat
     1021 cgacggcgaa ggcgtggttg atgctggggc gacgcgggtg ctgttgcaga tcttctccaa
     1081 gaccgtcatt ggtccgatct ttttcgagtt catccagcgc aaaggcgacg agggtttcgg
     1141 cgagggcaac ttcaaggcgc tgttcgagtc gatcgaagag gaccagatcc gccgcggcgt
     1201 gctcaaggcc tcctgaggcc gacgcaggcc cgaccaggcg acccgcgcct tgccctttgt
     1261 tttgcggatt ttgttgtttt gcaggttttg cgcgcgtggg ggctcagtcg ccgcgcacga
     1321 cgccgctgga ggccttgcgg ttataccact cggccgcgcc ggcgtggaac ggcaggaagc
     1381 tgttgcgcgt gaaattgccc ggtaccgtct cggcggccgc ggcgtggtag ctcaaaagtt
     1441 cctcgtgaca gctgaagacg gcatcgacga tcgcatagac gagatcgctg gggagcgccg
     1501 tgcgggcgac ggcgaaattg aaaaggccca cggtggaata gtggcgcagc agtgaggggt
     1561 aggtgcccgc cgcgacaagg gaggggccaa gctcgggcat ggcgagccgc agggtcacga
     1621 tctgttgtgg cgtcagcggc agatagcgaa ctttgatttt ccgttcgagc tcggcgaagg
     1681 ctggaaaggg ggcgccggcg gccacggcga gcgcatccaa cgagccatcc accaagcgcg
     1741 tggcgagctc ggcccagttg ccggtttgca gacgcgcatc gacctgcaag accttgaaga
     1801 attgcggcat gtagatgccg gtcgtgccgc cgctgggtcc catgcccacc cgcttgtctg
     1861 tcaggtcggc ggcgccgagg atggcgctgt cgcggggcac caggaactgg aagggcgtgt
     1921 cgtacatggg aaagatcgcg cgcagctggc gatagcggac accgttggtc cagtcgccgg
     1981 agccgttcca ggcctgctgc gccacgccca aggtgacaaa gccgaggtcg atctcgcccg
     2041 tctcgatcag cttgatgttt tcgctcgggc cctcggtcgg cctcgcccac accgtggttt
     2101 tgagctcgcg cgtgagaatg cgcgcgagcg cctcgccgta gacgaaatag gtgcctccgg
     2161 gcgaggcggt gccgatgctg agcgtcggcg gccaatgtgg ctcgcgcgcc tcgaccccgg
     2221 cgatagccgc gagcgccgcg ccgagcccga ggaacactcg aagcccgagg acgtaaaggg
     2281 cgtacatggc tttcctcccc ctagcgccga agccgagctg cgaggctcct cactgcatgt
     2341 gctggccgcc gttgatggcg atattggcgc cggtgacaaa ggccgcctct tcggagcaca
     2401 aatagatgat caagccggcg acctcctccg gtttgccgag ccgcgagatc gggatctgcg
     2461 gcaggatttt ggcttggagc acatcgttgg ggatcgccgt taccattttg gtgccgatgt
     2521 agccgggcga aatggtgttg acggtgacgc ctttgcgcgc cgactcgagc gccagcgatt
     2581 tggtgaagcc gtgcatgccg gccttggccg cggcgtagtt ggtctggccg aaggctccct
     2641 tggagccgtt gaccgaggag acattgatga tgcggcccca gccgcggtcc atcatgccgt
     2701 cgtagacctg cttggtcatg ttgaagactg aatcgagatt ggtcttgatc acggcgtccc
     2761 aatcggtctt gcccatctta cggaacgtca tgtccttggt gatgccggca ttgttgacca
     2821 ggatatcgac cggcccgacc tcggcggcca cccgcccgac acaggccttg ctcgattcga
     2881 agtcggtcac gtcgcaggca taggcgctga aggcgtagcc cttctgctgc atggcggcga
     2941 gccacttgtc ggaggttttg ttcgacggcg agtaggtcac gactacccgg tagccggcat
     3001 cggccatctt ggtgctgatg gtctcgccca ggccgcccat gccaccggtg atcactgcca
     3061 cgcgtttttg catgttcttc aactcctctt gggggaaaag gctaacgtac cggggtcggt
     3121 gggctcagca cgtccatcac gcgttccggc tcctcggcat cggcatccgt ttcgctatcg
     3181 agcaggcggc gcatgcggtc gttgtcgagc gcgccctccc agcgcgaaac caccagcgtg
     3241 gcgacgccgt tcccgatcag gttggtcagc gcccgcgctt ccgacatgaa gcggtcgacc
     3301 cccaggatca gggcaatacc ggccacgggg atggtgccga ccgatgagag ggtggcggcc
     3361 aaaacaataa agcccgagcc ggtcacgccc gcggcaccct tcgaggtcaa gagcaggatg
     3421 ccgatgatgc ccagctgctg ggccaggcta aggtcggtgt tggttgcctg cgccagaaac
     3481 agcgccgcca tggtgaggta gatacaggtg ccgtcgagat tgaaggagta gcccgtcgga
     3541 atgacgagac cgaccacgga tttctcgcag ccgaggttct ccatcttggc aatcatgcgc
     3601 ggcagcaccg actcggagga cgaggtgccg agcacgatca gcagctcctc tttgatgtag
     3661 cgaatgaatt tgaaaatatt ggcaccggcc agcatcgcga aaaagccgag cacgccgcag
     3721 atgaaaatga ggcaggtgag atagaaggcg atcatcagat gggcgagcga caccaatgtc
     3781 gacagcccgt acttgccgat ggtgaaggcc atggcgccga aggcaccgag cggggccacc
     3841 ttcatgatga tgccgaccac gccgaagaaa gcgtgcgagg cctgatcgat cacaccgagc
     3901 agggcctggc cgcgttgtcc cagggcctgc aagccgaagg caaacagaat agcgaagaac
     3961 aggacttgca ggatctcgcc ctgcgcgaag gctcccacca ccgtgtttgg aatgatgtcc
     4021 atcagaaact ggagcgtact ttgctccttg gacttggcga cgtagccttg gatggccctg
     4081 gtgtcgatgc tggtggggtc gacgttcatg cccccgccgg gctgcaacgt gttggccacc
     4141 agcaagccga tgatgagggc gagcgtcgtc accacttcga aatagaccag cgccttgagg
     4201 ccgacgcgac ccaccttgcg catatcctgc atgctggcga taccgtgcac gaccgtgcag
     4261 aaaatgatgg gcgcgatgat cattttgatc atcttgatga aggcatcgcc cagcggcttc
     4321 atttgttcgg ccaagtgcgg ttgaaagtgg ccaagcagca cgccggcgaa gatggcgacc
     4381 aagacctgga agtagaggct cttgaagaat ggcttgtgct tgggcggatc gggtatggcg
     4441 tgggcctgca tcgtcgcctc tccttgcggg ctttgctagc gttgcacgtg ggcgtcgacc
     4501 gcggcgatcg ctgtcatgtt gacgatgcgg cgcacgctta cggccggctc gaggatgtgg
     4561 gcgggtttgg ccgtaccgag cagaatgggc ccaaccgtaa tgccgctgcc agcggcgatc
     4621 ttcaggaggt tgtaggcgat attggcggca tcgacgctgg gcatgatgag aaggttggca
     4681 tcgctcccca gccgcgaatc cgggaacaca tcatccagca ccaccttcga cagggccgca
     4741 tcaccccgca tctcgccttc caccagtagg tccggcgcgc gctccaccag gagcgcgcgc
     4801 gcctcgcgca tcagctgtgc ctcgggtgcg gcggagctgc cgaagctcga atgcgagagc
     4861 agcgcgacga tcggggtcag attgaagcgg cgcacctctt cggcggcgag gagcgtcatt
     4921 tcggcaatgt ccgcggggcg cggctggtga ttgacatagg tatcgcagat aaagatctgc
     4981 cggttgggca gcatcagcat gttcatggcc gccagcgttc tgacgccggg gcgacggccg
     5041 atcacgtcct cgatgtagcc gaggtgctcg tcgaaggtgc cgaccgtgcc gcacagcatg
     5101 gcatcgacgt cgccacgtct gagcagcatg gccgcgatca aggaggcgcg gctgcgcatc
     5161 gcctccttgg cgatggcctc cgtgacaccc ttgcgccgcc ccagctggta gtactccgcg
     5221 accacctggg ggtaacgggg atcgttcagg atattgacga cctcgcagcc ggcgccgagt
     5281 ttcaggcgca gacccaaggc gtcgatgcgc ttggcgatca cgtcggcgcg gccgacgagg
     5341 acgggccgtg ccaggccttc gtcgaccacc acctgcacgg cgcgcagcac gcgctcttcc
     5401 tcgccttcgg caaagatcac ccgcttcggc gccagctttg ctgcctcgaa caccggcttc
     5461 atgctgttgc cggaatggta gacgaagcgc gccagctgct ggcgataggc ctccaggctg
     5521 gccagtgggc ggcttgccac gccgctatcc atcgccgccc gcgccaccgc cggcgcgatg
     5581 cgctcgatca gacgcgggtc gaaggggcgg ggaataaggt aatggcgccc aaacgtcagg
     5641 gattggccgc cataggcgct ggccacgatg tcggacgcct cggcctgggc gagctcggcg
     5701 atcgccttca cggcggcaat cttcatggcc tcgttgatgc ccgtggcgcc gacgtcgagc
     5761 gcgccccgaa agataaaggg aaagcacagc acgttattga cctgattggg ataatcggag
     5821 cgtcccgtgc acaggatggc gtcgggccgg gccgcaaggg cggcctcggg cctgatctcg
     5881 ggctccggat tggccagcgc caagatcagc gggtccttgg ccatgggcac gatcatctcg
     5941 gcgcgtaaca cgccgcccgc ggacaaccca aggaacaggt cggcgcccgg caggatctcg
     6001 gccagcgtgc gcgccttggt gtcctgcgcg tagcggatct tctcgctatc catctccgcg
     6061 gcgcgaccct taaagaccac gccatggatg tcggacagga acacgttctc gcgcttgagc
     6121 ccaagcgccg tgagcagatc gaggcaggcc agcgcggcgg cgcccgcccc ggaaacggca
     6181 accttgatgg cggtgatgtc tttgccgacg agcttgagcc cgttcagaac cgcggcggag
     6241 acgatgatgg cggtgccgtg ctggtcgtca tggaatacgg gtatcttcat gcgctcgcgc
     6301 agcttgcgtt cgacgatgaa gcattccggc gccttgatgt cttcgaggtt gatgccacca
     6361 aaggtcggct cgagcgcggc cacgacagcg acgatgtgat caggctccgt ggcatcgatt
     6421 tcgatgtcga acacgtcgat gccggcaaac tttttgaaga ggcaggcctt gccctccatc
     6481 accggcttgc cggcgagcgg accgatgttg cccaagccca ggaccgccgt gccattgctc
     6541 accaccgcga ccaggttggc gcgcgaggtc aatagtgcgg cctgctgtgg gtcgtcgcgg
     6601 atggcaaggc aggcgtaggc cacgcccggc gaataggcga gcgagaggtc gtgttgattg
     6661 gtgagcccct tggtcggcga aattgctatt tttccggcga cgggagcgcg atggtagtcg
     6721 agggcagctt cgcgaagcag tttttcgtcc atcggatccc cagcgctctc tcactcgcac
     6781 aggctctttt acgcaacggc caaatacgtg gaggatattg cctcacgccc gcggcgtcag
     6841 atagcggttg tgcagacttg ggcccatctc ctcgcacacg gcgcggtcag cgggctgccg
     6901 ttgcggcggt cgggaccgcc ttggtgggca ggccgtgcgg cacggctgtg tccaaggtct
     6961 aaatctcggc acgagaaaga tagagaggcg tggcgatggg gatggttaat gtcgcgggcc
     7021 cattgcgcgc cggcgaccgg cctgccggaa aagacgagaa gccggagcag acgaaaaaaa
     7081 tgtgacgctg ggggaacttg gcttcgcctc gggcattccc agggcatgca tatgcttggg
     7141 caggccgccc ggctttcccg tcaggacgag acctgtttcg ctggccgcgc tctcctagtc
     7201 agagcgcggc ctttgcgcgt ctgaaggggg gcgcggcggc tcggctgccg gcagcccttg
     7261 tcggcaactg ctcgggcatg ccgcgctgct gagccatgcc cgcgcgttgc gccggcgcct
     7321 caagagactt tcaccattcg cttgccgcga ttttggccgc cgagcaggcc gatcaaggcc
     7381 gccggcgtat tttccaggcc ttcgatcacg tcttcctgca ccttgagttt tttcgccgcc
     7441 acccattcgc caagctcggc gatcgctgcc gcgcgttccg cgtaatagtc ggtgacgatg
     7501 aagcccttga gcagcaggcg cttgacgaca atgaggccgg gcacgccacg tgggccatgc
     7561 gccggggcgg ggccgtcata ggcggaaatg gcgccacagc aggcaatacg accgccaatg
     7621 ttcatctgtg ccagacaggc ctcgaggatg tcgccaccga cattgtcgaa gtagacgtcg
     7681 agcccggacg gtgcggccgc cttcaaggcc ttgaagaccg gccccgcctt gtagtcgacc
     7741 gcggcatcga agcccagctc ttgcagcagc caccggcact tgtcctcgcc accggcgatg
     7801 ccgaccacgc ggcatccctt gatcttggcg atctggccga cgagcgatcc gaccgagccc
     7861 gccgccgcgg agacggcgac cgtctcgccc gcccgcgggc cggcaactct caagagaccg
     7921 aaataggcgg tgagcccggc aatgccatag acgctgagga gatgcgtgat cggctcgacg
     7981 ctgggcaaca ggatcagacg tttggccggc agtgccgcgt actcctgcca gcccgtatcg
     8041 gcaaagacga gatcccctgg tgcgaagccc tgggccttgc tctccaccac ctccgccacg
     8101 gccgcaccgg ccatgatgtt gccggcttcg acggcgtcgc gataggtggc gccctgcatc
     8161 caggcgcggt tggcggcatc gagcgagatg tagcgcacct tcaggcccac ctcgccgtct
     8221 gcgggggagg ggatgggggc ctcggttagg gcaaaatgct cgagcgcgag tttgtccttg
     8281 gggacttgca cgagccgaat ttgtcgattg ctgcgggcca tcccgatcac ctccagcttg
     8341 atgccgtcgt cgccatgatg cggcgcggcg ccgagctttt gcaacccgcg gggtcgttcg
     8401 ggccgcccgg ccggacccat ggccgatccc cgcgccgacc gcagggccga tcacgatttg
     8461 ccgatgacgc acccggcggc ttacaaagga gcgcggcttg gcagtacccg tgccgtgcct
     8521 ggccgtccca ggccaaggga aaaaggttat ggcaaggacc ttcgctttaa gccgccgccg
     8581 gttcctcgcc tctggcggcg ccttggttgc cagcggtttg agcggcgcgg ccgcgggccc
     8641 ggatgctgcg gcccccgcac cggcctcgtt tcgcctggcg cttggcgcgc acgagctcat
     8701 cgtcttgagc gacgggcacc tcgtcgtgcc cacggccatg ttggccagaa acgtgggcgg
     8761 cagcgaactt gcggcctatc tcgccagcgt tgggttcggc cgcgagcgcg tgcatttcca
     8821 tatcaatgtc gcgctgatcc gaacgggcga tgagcacatc ttgatcgatg cggggtcggg
     8881 cggcacctgg gagccgacgg ccggccggct cttcgacagc ttggaggccg ccgggatcgc
     8941 gcccgcggcg atcggcaagg tcgtgatcac ccacgcccat cccgatcatc tctggggttt
     9001 gatcgacgag ctcgacaaca gcct
//
LOCUS       DASTMT010000168        13197 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_2388847, whole genome shotgun sequence.
ACCESSION   DASTMT010000168 DASTMT010000000
VERSION     DASTMT010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 13197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13197
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2388847"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..411
                     /locus_tag="VFR00_03715"
     CDS             <1..411
                     /locus_tag="VFR00_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008873037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEU0158391.1"
                     /translation="HVFGYTLFNDISVRDFQRKSSQWTAGKNFDATGPLGPWVVTADE
                     LPAGAAGLRIMTRVNGEIMQDSNTSDMIFSSARIIALLSEFMTLEPGDVIATGTPSGV
                     AHARKPPTWLRAGDKVEVEVEKIGVLANPIVEER"
     gene            complement(422..1675)
                     /locus_tag="VFR00_03720"
     CDS             complement(422..1675)
                     /locus_tag="VFR00_03720"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011995124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEU0158392.1"
                     /translation="MKFGIFYELQLPKPWGPDDERQLVQDALLQVELADRLGIDYAWA
                     VEHHFLEEYSHCSASEVFLAAAAARTRRIRLGHGIRQVIANYNHPARTAEAIAMLDLV
                     SNGRAELGIGEGATRLELGGFNIPAKEKRAMSLEAAEQIANMLVMTPYPGFEGRSFKM
                     PCRNVLPKPTQKPHPPLWMACTNRDTIKVAASNGLGALAFSFIDEAEARTWSRIYYDI
                     IKSEACVPLGHSVNANIAMVSAFSLHADASEAARRGQEGFEFFRYAISALVSKDTVPG
                     RSRLFEQFQAERNSGALAGTVADTESLASAFQRSGGIGTPAEFREHVRSFEAAGIDQI
                     ILLQQAGRNKHADICESLELLAREVLSEFGAKSAERERRKAEALAPFIEQALARKPRL
                     PALTDADIPVVRASVAKPLVNQSAS"
     gene            complement(1884..2669)
                     /locus_tag="VFR00_03725"
     CDS             complement(1884..2669)
                     /locus_tag="VFR00_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008139065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF169 domain-containing protein"
                     /protein_id="HEU0158393.1"
                     /translation="MPTLIADLASRLETLLRLRSIPFAMKLFANRAEMEAIPRIRRPS
                     SVHTLDQVVAQAARLGWTVGVTAEDLVGAQCRAVVGLGAAKDAAWRSGKHMSGVWFAS
                     PEDAAAHQAAMHCVEDGQYDALAVAPLASGRLDPADIALFYATPGAMMYFINGLQWSG
                     YKNFEWGVVGESACADSWGRALKLRQPSLSIPCFAERRYGGVLDDEMLMALPPEFLSK
                     AIAGMDALAKNGFRYPFPQYGIQQDVRAGMARSYPDRAAPKGS"
     gene            complement(2826..3593)
                     /locus_tag="VFR00_03730"
     CDS             complement(2826..3593)
                     /locus_tag="VFR00_03730"
                     /EC_number="2.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0018104 - peptidoglycan-protein
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEU0158394.1"
                     /translation="MRILRLLCRSAIAAGVGAVATGWQIAPASAQLFWDWGGGSMVGG
                     SGREVVSFSPQYAKGQIIVSFGDRRLYYITQPGQALSYPIAIPREQSRWQGVTSVSDK
                     RVNPSWTPTPTMVAENPRLPRWVPGGHPMNPLGVRAMYLGSSTYRIHGTDAPWTIGTA
                     VSKGCIRMYNQDVLDLYPRVPVGTKVTVTWDRFTAVASYSPPGGPPAGKSSGYNASST
                     NPSSSGGFFNLFGDDDLPPEKPRRPVRPKTNRSASTE"
     gene            3951..5786
                     /gene="ggt"
                     /locus_tag="VFR00_03735"
     CDS             3951..5786
                     /gene="ggt"
                     /locus_tag="VFR00_03735"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020187397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEU0158395.1"
                     /translation="MRPDLRRARGPHDLETPARALRRGRRPHGRALLGILGLLGGLAS
                     PPAWGLDPRAAPEAATGWYGQAVATAKQQMVATANPYATEAGLKILGQGGSAADAAVA
                     VQLVLNLVEPQSSGLGGGAFVLFWDAARQRLAALDGRETAPAEAAPDQFLEGGRARPF
                     DEAVYGGLSVGVPGTLRLLQMLHQRHGRLAWADVLAPAIQLASDGFRVSPRLHLLLRG
                     YGAQNFAPAARQYFFDATESARPAGHLLKNPSFAATLKAVAEGGADAFYAGAVAEAIV
                     AAVRGAPNHQGRMTLADLAGYRAVEREPLCVSYRPYRVCGMPPPSSGGLAVAQILKLI
                     EPFEIGRQPADAMNAAALHRITEAEKLAFADRDHYVADPAFVSPPAGLLDASYLARRR
                     GLIDLSTAMPRPRPGNPQQQSWRQLGEDASVEAAGTSHFSILDAAGNMLAMTSTIEAA
                     FGSRLWAAGFLLNNELTDFSFRPVDSSGRPLANALAPGKRPRSSMAPTIVFDATGKPW
                     AALGSPGGSRIILYVVKALVGLMDWQLDAQAATALMNFGSRGGAFEIEIDHPAAIWHA
                     LKMKPYGHLVSADLLTSGTHLIVVRPNGLIEGGADPRREGLAQGE"
     gene            5833..6834
                     /locus_tag="VFR00_03740"
     CDS             5833..6834
                     /locus_tag="VFR00_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011440854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU0158396.1"
                     /translation="MQDAVITVVGAGITGLWQALTLARRGHRVRLIERATTPFAAAAS
                     RFAGAMLAPYCEAETAAPVVFELGLKSLALWRNTFPAALWNGTLVLAAARDKGELAHY
                     ARLTQGHRRLDAAAIAELEPDLAQRFDQGLLYVEEGHLEPLAAMHFLLAAGRAAGVEV
                     LLGTTWQPQMRAVGDLVIDCRGLDARGELLALRGVRGERLIVRSREVALSRPVRLLHP
                     RHPLYVVPWDNGHYMLGATVIESDDAGPITVRSALELLGTAYALHPAFGEAEIVEAGA
                     GVRPALPDNVPKIVVRGRTIFVNGLFRHGFLLAPVLSVVLADYLEKGAIEPGVFEIE"
     gene            7125..8192
                     /locus_tag="VFR00_03745"
     CDS             7125..8192
                     /locus_tag="VFR00_03745"
                     /inference="COORDINATES: protein motif:HMM:NF012774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HEU0158397.1"
                     /translation="MFAWRKKTEGFEWHQYIRTAVRHRRQQRHDRMVEARRAAGQQVQ
                     AAGVALAAGSKAAGVAAKEGAKASLGAAGLLAGGLSSMLLALCRIAVRAVLIAAEPVV
                     AALARPLIGGAVGFAGGLVLGAGIGRARALGFDAEAYTTFAIAALLFIATLPLVSALT
                     GLRLPRLGGAALQPAALLLGIAVLALAAAGAWYAKGGRLSVANLGASLPLIGGGRPLE
                     GRAYAVGGDSLQIGGTTVRLAGIEAPEQEQRCGAGSRRWRCGAAASDALSRLVNGRIV
                     SCALSGNDAKGRPLANCSAGKMEINAELVRRGHVFAESGLFARYASLENEARAAKIGL
                     WAGGDVERPSEYRARQKRPGV"
     gene            8292..8504
                     /locus_tag="VFR00_03750"
     CDS             8292..8504
                     /locus_tag="VFR00_03750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0158398.1"
                     /translation="MTSQETNPKPAPAAGEDTRARSPNLVPMLVVLFAVLSAVVLFAL
                     DRDSKSSAPQPNYRADAPVTAPAAPR"
     gene            complement(8522..9922)
                     /locus_tag="VFR00_03755"
     CDS             complement(8522..9922)
                     /locus_tag="VFR00_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011440335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HEU0158399.1"
                     /translation="MHPNLVALLYLAAGVLFILALRGLSSPESSRRGNLYGMVGMTIA
                     VVTTLAQLGDTSLFTWVLILVGLGIGGGIGATIARRIPMTSMPELVAAFHSLVGLAAV
                     FVAAGALYAPEAFGIGHFRAIAGASLLEMSLGVAIGAVTFTGSVIAFAKLSGRMSGKP
                     IILPARHLINAALAGLLVVLIAWFCRSGGDPVLFWAITAAALLFGVLIIVPIGGADMP
                     VVISMLNSYSGWAAAGIGFTLGNIALIVTGALVGSSGAILSYIMCKGMNRSFVSVILG
                     GFGGETAGPAAGAEQKPVKVGSAEDAAFLLKNSGKVIIVPGYGMAVAQAQHALREMAD
                     HLKEAGVEVKYAIHPVAGRMPGHMNVLLAEANVPYDEVFELEDINNEFAQADVAYVIG
                     ANDVTNPAAEDDKSSPIYGMPVLQVWKAGTVMFNKRSLASGYAGIDNPLFYRDNTMML
                     LGDAKKMTEEIGKAMD"
     gene            complement(9939..10391)
                     /locus_tag="VFR00_03760"
     CDS             complement(9939..10391)
                     /locus_tag="VFR00_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-translocating transhydrogenase family
                     protein"
                     /protein_id="HEU0158400.1"
                     /translation="MTRALAWPDPKIQLRQVTRGAGAARGRGLALLALLFALLPAPAM
                     AVGGSDVSPFVYQFTVFVIAIFVGYYVVWSVTPALHTPLMSVTNAISSVIVVGALLAV
                     GVPLVEAGSGLAKTFGFLALTMASVNIFGGFLVTQRMLGMYKKREPKR"
     gene            complement(10393..11532)
                     /locus_tag="VFR00_03765"
     CDS             complement(10393..11532)
                     /locus_tag="VFR00_03765"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008746 - NAD(P)+ transhydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HEU0158401.1"
                     /translation="MVTIAVTAEGKGEPRVAVSPETVKKLTALGADVRVQQGAGSRSR
                     FSDAALQAAGAVVSPDAAATLSGADILLKVRRPSLGEVEALKRGAIVAAMLAPFDDRP
                     GLEALAGAGAALLSMEFMPRITRAQSMDVLSSQANLAGYKAVVDAAAMFGQAIPMMMT
                     AAGTVPAAKVFIMGVGVAGLQAIATARRLGAIVSATDVRPATKEQVASLGAKFIAVED
                     EEFKQAQTAAGYAKPMSAEYQKKQAELIANHIKAQDIVVTTALIPGRPAPKLISAAMI
                     ESMKPGSVIVDLAAERGGNTELTQADKVVEHKGVSIAGPLNLAGEVPVNASALYAKNL
                     FAFLETMFDKKDKVLKVNWDDEIIKGTLVAKDGQLVHPSLQQQGA"
     gene            complement(11620..11733)
                     /locus_tag="VFR00_03770"
     CDS             complement(11620..11733)
                     /locus_tag="VFR00_03770"
                     /inference="COORDINATES: protein motif:HMM:NF019452.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aa3-type cytochrome c oxidase subunit IV"
                     /protein_id="HEU0158402.1"
                     /translation="MDYPEHLRTYAGFLKATVALIVGVALLLLFMLFYLVP"
     gene            12042..>13197
                     /locus_tag="VFR00_03775"
     CDS             12042..>13197
                     /locus_tag="VFR00_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008195739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEU0158403.1"
                     /translation="MDLGMTAKVKPLVEKVRAMVRDEIMPLEAEYEAEVGKGDRWTYT
                     KRQEEIREDLKAKARERGLWNFWLTGSDKGYGLSTVEYAYLAEEMGWSRLAPETFNCS
                     APDTGNMEVIERYGTPAQKEQWLKPLLEGKIRSAYIMTEPNVASSDATNLALDAKLDG
                     EEWVLNGEKWFASGAGDPRCKIYIVMVCSDPTADKHKRHSQIFVPAGTPGVEILRPMK
                     VYGDDDAPHGHMHVRFTNVRVPKENLILGRGRGFEVSQGRLGPGRIHHCMRAIGQSER
                     ALELLCKRSLSREAFGKPLAQLGANYDIIAESRMKIEMARLLCLKAAHMMDTVGHREA
                     SPWISQIKVIAPRIALEITDEAVQMHGAMGITQDTPLAHMWTHLRTLRLAD"
BASE COUNT         2198 a         4320 c         4415 g         2264 t
ORIGIN      
        1 cacgtgttcg gctacacgct tttcaacgat atttcggtgc gcgacttcca acgcaagagt
       61 tcgcaatgga ctgcgggcaa gaatttcgac gcgacgggtc cgcttggacc ttgggtggtc
      121 accgccgacg agttgccggc aggcgccgcc ggcttgcgca tcatgacgcg cgtcaacggc
      181 gagatcatgc aggattccaa cacgtccgat atgatcttct caagtgcccg catcatcgcg
      241 ctgttgtcgg agttcatgac gctggaaccg ggagatgtga tcgcgaccgg tacgccttcg
      301 ggcgtggccc acgcccgcaa accgccgacc tggctgcgcg ccggcgacaa ggtggaagtc
      361 gaggtggaaa agatcggcgt gctggccaat ccgatcgtcg aggagcgttg agcgcggcca
      421 gctaagacgc cgactggttg accagcggct tggctacgga ggcgcggacc acgggaatgt
      481 cggcgtcggt tagcgccggc aggcgcggct tgcgcgccag cgcctgttcg atgaagggtg
      541 ccaatgcctc ggccttgcgg cgctcgcgct cggcgctctt ggcgccgaat tcgctcagca
      601 cctcgcgggc caagagctcc agcgattcgc aaatatccgc gtgcttgttg cggccggcct
      661 gttgcaagag gatgatctgg tcgatgcccg ccgcctcgaa ggagcgcacg tgctcgcgaa
      721 actcggccgg cgtgccgatt ccgcccgagc gttgaaaggc gctggcgagg ctctccgtat
      781 cggccacggt ccctgccagg gcgccgctat tgcgctccgc ttggaactgt tcgaacagcc
      841 gcgaccggcc gggtacggtg tccttgctga cgagcgcgga aatggcgtag cggaagaact
      901 cgaacccctc ctgaccacgt cgcgcggcct cgctagcgtc cgcgtgcagc gagaaggctg
      961 acaccatggc gatgttggca ttgacgctat ggcccagggg aacgcaggcc tcgctcttga
     1021 tgatgtcgta gtaaatgcgg ctccaggtgc gcgcctcggc ctcatcaatg aaggagaagg
     1081 cgagcgcgcc aaggccattg ctggccgcaa ccttgatggt gtcacggttg gtgcaggcca
     1141 tccacagggg cggatgtggc ttctgggtgg gcttggggag cacgttgcgg cagggcatct
     1201 tgaacgaccg accctcgaag ccgggatagg gggtcattac cagcatgttg gcgatctgct
     1261 cggccgcctc gagcgacatg gcgcgctttt ccttggccgg aatgttaaag ccgccgagtt
     1321 cgagcctggt ggcgccctcg ccaatgccca actccgcacg cccattcgag acgaggtcga
     1381 gcatggcaat ggcctcggcc gtgcgcgcgg gatgattata attggcgatc acctggcgaa
     1441 tgccgtggcc gagccgaatg cgccgcgtgc gcgcggccgc ggcggccagg aacacctccg
     1501 acgccgaaca gtgggagtac tcttcgagga agtggtgctc caccgcccag gcatagtcga
     1561 tgcccaggcg atcggcgagc tccacctgga gcaaggcatc ttgcacaagc tggcgctcgt
     1621 cgtcggggcc ccaaggcttg gggagctgca gttcgtagaa gatcccgaat ttcatgcggc
     1681 gtgctcgcgt caaagcccgt cctggtcagc gcaagaagcc taagcaggat cgaccgcctt
     1741 gccaacacgc ggctggcccg tgcacccggt catggccgag ccgccggccc tgccaagcca
     1801 gcccttgcag gcgcgggccc gcgcgcatgg cacgcagagg cggtgggcca tggtcaagcg
     1861 ccgacgccgc ctcgcctgct tttttagctg cccttgggcg cggcgcggtc cggatagctg
     1921 cgggccatac cggcgcgcac atcctgttgg atgccgtatt gcgggaaggg atagcggaag
     1981 ccgtttttcg ccagcgcgtc catgcccgcg atggctttgg acaagaattc cggcggcagc
     2041 gccatcagca tctcgtcatc gagtacgccg ccgtagcgcc gctcggcaaa acaaggaatc
     2101 gatagcgatg gctggcgcaa tttgagtgcg cgcccccaac tgtccgcgca ggcgctctcg
     2161 cccaccacac cccattcgaa gtttttgtaa ccggaccatt gcaagccatt gatgaagtac
     2221 atcatggcgc cgggcgtggc gtagaagagg gcgatatcgg ctggatcaag ccggccggag
     2281 gccagcggcg ccactgccag ggcgtcatac tggccatctt caacgcagtg catcgcggct
     2341 tggtgcgcgg cggcgtcctc agggcttgca aaccagacgc cgctcatgtg cttgcccgag
     2401 cgccaggccg catccttggc cgccccgagc ccgaccacag cccgacactg cgccccgacc
     2461 aggtcctcgg ccgtaacgcc gacggtccat cccaaacgtg ccgcctgtgc cacgacctga
     2521 tcgagggtgt gcacgctgct cggccggcgt attcggggga tcgcctccat ctcggcccgg
     2581 ttggcgaaca gcttcatggc gaacgggatc gaccgcaggc gcaacagggt ctccagccgg
     2641 ctggcgagat cggcgatgag ggtcggcatg agcaaggctc cgctggcggg cgcaacgcat
     2701 gcatcccttg taccggctcg aggggcatgg gcgccagtca ggcgtgctga cccccctatg
     2761 ccggcggcag gagcgcgcgc ccgcagcgca acggtggtgg ctgcgcggcg cgcgtgggca
     2821 gaggcttatt cggtcgaagc gctacggttc gtcttggggc gcacgggtcg gcgcggcttt
     2881 tctggcggca gatcgtcatc cccgaagagg ttgaaaaagc caccgctaga ggacggattg
     2941 gtgctcgagg cgttgtagcc gctggacttg ccggccggcg gaccgcccgg cgggctatag
     3001 ctcgccaccg ccgtgaagcg atcccaagtg acggtgacct tggtgccgac cgggacacgc
     3061 ggatagaggt cgagcacgtc ttgattgtac atgcgaatgc agcccttcga caccgccgtg
     3121 ccgatcgtcc aaggcgcatc ggtgccgtgg atgcggtagg tactggaccc caggtacatg
     3181 gcgcgcaccc ccagggggtt catcgggtgc ccgcccggca cccagcgcgg cagccgtgga
     3241 ttttcggcca ccatggtcgg tgtcggagtc catgacggat tgacacgctt gtcggacacg
     3301 ctggtcacgc cctgccagcg gctctgttcg cggggtatgg cgatggggta gctcaacgcc
     3361 tggccgggct gcgtgatata gtagagccgt cgatcgccga aactgacgat gatttggcct
     3421 ttggcatact gcgggctgaa gctgacaact tcccgaccac tgccgcccac catgctgccg
     3481 ccgccccaat cccagaagag ttgcgcggac gccggggcga tttgccaccc cgtcgcgacg
     3541 gcgccgactc ccgcggcaat agcgctgcgg cacagcaagc gtagaatgcg catcgtctca
     3601 ggctcccgtg ttctttttgc ctgtccctct tgcctcggcg aacaatcttg cctcggctcc
     3661 ggtgaaaagt gccttagcaa aagttcggat tggtcgccgc tactggctcc gtggcctgga
     3721 gcccatcgac aggtgcccgc aacccttgag atccaatccc aagcgcctcg ccccgctcga
     3781 cgcagagcag agtgtggggc gcgaggtgcc cggacgcaac ccttcggacc tagtccaagg
     3841 tattgaaaag ccacgatcgt gacgctgttg tgcgatttga gccacatggg agaaactgtg
     3901 cacaagcttt gcctgcattg gccgcccgcg ccgcctcggg cctcctgcgc atgaggcctg
     3961 accttcgacg ggcgcgcggc ccgcacgacc tcgaaacgcc cgccagggcg ctgcggcgcg
     4021 ggcgccggcc ccatgggcgg gccctgctcg gtatcttggg cctcctcggg ggcctggcgt
     4081 cgccgccggc ctgggggctc gatccgcgcg cggcgcccga ggccgccaca ggctggtatg
     4141 ggcaagccgt cgccaccgcc aagcagcaaa tggtggcgac ggcaaatccc tatgccaccg
     4201 aggcggggct caagatcctg ggccagggtg gcagcgccgc cgatgcggcg gtggctgtgc
     4261 aactcgtctt aaatttggtc gaaccacagt cgtccgggct cggcgggggg gcctttgtgc
     4321 tcttctggga tgccgcgcgc caacgtttgg cggccctgga cgggcgcgag acggctcccg
     4381 ccgaggccgc gcccgaccaa ttcctcgagg gcgggcgcgc gcgtcccttc gatgaggctg
     4441 tgtacggcgg gctcagcgtc ggcgtgcccg gcacgttgcg cctcttgcag atgctgcatc
     4501 aacggcacgg ccggttggcc tgggccgatg tgctggcgcc cgcaatccag ctggcgagcg
     4561 atggctttag ggtctccccg cgccttcacc tgttattgcg cgggtacggt gcgcagaact
     4621 ttgctcccgc cgcacgccag tattttttcg acgccaccga aagtgcgcga ccggcgggcc
     4681 accttttgaa gaaccccagc tttgcggcaa ccctgaaggc cgtggcggag gggggcgccg
     4741 atgcatttta tgcgggtgcc gtcgccgagg ccatcgtcgc cgccgtcagg ggcgcgccca
     4801 atcatcaggg tcgcatgacg cttgcggatc tggctggcta tcgcgccgtc gaacgcgagc
     4861 ccctctgcgt cagctatcgg ccttaccggg tttgcggcat gccaccgccg tcgtccggtg
     4921 ggctggcggt tgcgcagatc ctgaagttga tcgagccgtt cgagatcggc aggcagcccg
     4981 ccgatgccat gaacgcggca gcgctgcatc gtattaccga ggccgagaag ctcgccttcg
     5041 ccgaccgcga ccactacgtc gccgatcccg ccttcgtgtc cccgccggcg ggcctgctcg
     5101 atgcctccta tctggcgcgc cggcgcggcc tcatcgacct gtcgacggcg atgccgcgtc
     5161 cgcgtccggg taacccgcag cagcagagct ggcgccaatt gggcgaggac gccagcgtcg
     5221 aagccgccgg gaccagccac ttctcgatcc tcgatgcggc cggcaacatg ctggccatga
     5281 ccagcacgat cgaggctgcc ttcggctcgc gcctatgggc ggcgggattc ctgctgaaca
     5341 acgagctcac ggatttctcc ttccgacccg tcgacagcag cggtcgcccc ctggccaatg
     5401 cgctggcgcc aggcaagcgg ccgcgcagct ccatggcacc gaccatcgtc ttcgacgcga
     5461 cgggtaagcc gtgggcggcg ctcggctcgc ccggcggctc gcgcatcatc ctctatgtcg
     5521 tcaaggcgct ggtggggctg atggactggc agctggatgc ccaggccgct accgcgctca
     5581 tgaacttcgg tagccggggc ggtgccttcg aaatcgaaat tgaccatccg gcggcgatct
     5641 ggcatgcgct gaaaatgaag ccctatggac acctcgtgag cgccgacctc ttgacctcgg
     5701 gtacccacct catcgtcgtg cgcccgaacg gcctgatcga aggcggggcc gacccgcgcc
     5761 gcgagggttt ggcgcaaggg gagtgagcgc cggcgctcgt cacatgggcg cgggccggca
     5821 acaattgaga tcatgcagga cgccgtgatc acagtggttg gcgccggtat caccggcctc
     5881 tggcaggccc tgacgctggc gcgccgcggc caccgggtgc gtctgatcga acgggcgacg
     5941 acgccgttcg ccgctgccgc cagtcgcttc gcgggcgcta tgctggcgcc ctattgcgag
     6001 gcggagacgg cggccccggt cgtgtttgag ctcggcctga aatcgcttgc tctgtggcgc
     6061 aataccttcc cggccgccct ctggaacggc acgctggtgc tggcggcggc gcgcgacaag
     6121 ggcgagctcg cgcactatgc gcgcctcact cagggccatc gtcgcctcga cgctgccgcc
     6181 atcgccgagc tggagcccga tttggcccag cgcttcgacc agggtctgct ttacgtcgag
     6241 gagggtcacc tcgaaccgct ggcggccatg cacttcttgc ttgcggcggg gcgggctgcc
     6301 ggggtggagg tcctgttggg cacgacctgg cagccgcaga tgcgcgccgt tggggatctt
     6361 gtcatcgact gccgcggtct cgacgcacgc ggtgagctct tggccttgcg cggggttcgc
     6421 ggcgagcggc tgatcgtacg ttcgcgcgag gtggcgctga gccggccggt gcggctcttg
     6481 catccgcgtc atcctcttta cgtcgtgccc tgggacaacg gtcattacat gctgggcgcc
     6541 acggtgatcg aaagcgacga cgcgggcccc attacggtgc gttcggcgct cgagctcctg
     6601 ggcaccgcct atgctttgca tcctgccttc ggggaggcag aaattgtcga agcgggtgcc
     6661 ggtgtgcgtc ccgccttgcc cgacaacgtg ccaaagatcg tggtgcgggg acggacgatc
     6721 ttcgtcaacg gtctcttccg ccacggcttt ttgctggcgc ccgtgctctc ggtggtgctg
     6781 gcggactact tggagaaggg cgcgatcgag cccggcgtgt tcgagatcga atagcacccg
     6841 ggccagggcg cgtcggcgcg aaccctgggc cgacaggggc cgtgatttgt tcggccgcgc
     6901 tggccgctgc ggccaaggtc atgctggcgc ctgccgacta gggcgggccc gcatggcccg
     6961 cttgcgccgg cgccctattg ccgccggcgg actattgatc ggctcgcgga tttaacgttc
     7021 cattcacgcc acgattcggc cgtggcgctg ggcttagtcg agcccaggtg cgggttcatg
     7081 cgttcgatct tcgcgtgtat cacctcgcgg gtgaggagga tgccatgttc gcttggcgca
     7141 agaagaccga gggttttgag tggcaccagt acatccgcac cgccgtgcgc cacaggcgcc
     7201 agcagcgcca tgaccgcatg gttgaggcac gacgcgccgc gggacagcag gtgcaggccg
     7261 ccggcgtcgc gctggccgct ggctcgaagg cggcaggggt ggcggccaag gagggtgcga
     7321 aggcgagcct cggcgccgcc gggctgctgg caggcggcct ttccagcatg ctgctggcgc
     7381 tatgcaggat cgcggttcgc gccgtcctga ttgccgctga gcccgtggtc gccgcgctgg
     7441 cgcggccgct gatcggcggt gctgtcggct ttgcgggcgg gctggtgctc ggcgccggga
     7501 ttggacgcgc gcgcgcgctg ggtttcgatg ccgaggccta tacgacgttt gcgatcgccg
     7561 cgctgctctt catcgcgacg ctgcctctcg tctcggcgct gacgggcttg cgcttgccgc
     7621 ggctcggggg agccgccctg caaccggccg cgctgttgct cggcatcgcc gtcctggctc
     7681 tcgccgcggc cggtgcctgg tacgccaagg gcggtcgcct cagtgtcgca aacctcggcg
     7741 cgagcctgcc cctgatcggg ggcggccggc cgctcgaggg ccgagcctac gcggtcggcg
     7801 gcgacagctt gcagattggc ggcaccaccg tgaggctcgc gggcatcgag gccccggagc
     7861 aggagcaacg gtgcggcgcg ggtagtcggc gttggcgctg tggcgccgcg gcgagcgatg
     7921 ccctctcgcg cctggtcaac gggcgtattg tcagctgtgc gctctcgggc aacgacgcca
     7981 agggacggcc cttggcaaac tgcagcgcgg gcaagatgga gatcaacgcc gagcttgttc
     8041 gacgcggcca cgtcttcgcc gaaagcggct tgtttgcgcg ttacgcctcg ctcgaaaacg
     8101 aagcccgcgc ggccaaaatc gggttgtggg cgggcggaga cgtcgagcgt ccctccgagt
     8161 atcgcgcccg ccaaaagcgc cccggcgtct gaggccgcgc cgatcctctt tgctgcccgc
     8221 gttggcccgg cgcatcgcgg gaaccgggga tgcacgctcc gagttacgcg tttttgcgag
     8281 ttggagccgc catgaccagc caggaaacga acccgaagcc tgcgcccgcc gccggcgagg
     8341 acacacgcgc taggtccccc aatctcgtgc ccatgctcgt ggtcttgttc gcggtgctat
     8401 ccgccgtggt gctgtttgcc ttagatcgcg acagcaaatc gtcggcgccg caacccaact
     8461 accgcgccga tgcgccggtg acagcacccg ccgccccgcg ctagtgcggg gcgcgggcgc
     8521 ctcaatccat ggctttgccg atctcctcgg tcatcttctt ggcatcgcca agcagcatca
     8581 tggtgttgtc acgatagaag agggggttgt cgatgccggc ataaccggac gccagcgagc
     8641 gcttgttgaa catgacggtg ccggccttcc agacctgaag cacgggcata ccgtagatgg
     8701 gtgaggattt gtcatcctcc gccgccggat tggtcacgtc gttggcgccg atcacgtagg
     8761 cgacgtcggc ttgtgcgaat tcgttgttaa tatcttccag ctcgaacact tcgtcgtaag
     8821 gcacgttggc ctctgccaag agcacgttca tgtgccccgg catgcgcccc gccaccggat
     8881 ggatggcata cttcacctcc acgcctgctt ccttgaggtg atcggccatc tcgcgcaacg
     8941 cgtgctgggc ctgcgccacg gccatgccgt agcctggtac aatgatcacc ttgccggaat
     9001 tcttgagcag gaaggccgcg tcctccgccg agccgacctt gaccggcttt tgctcggcac
     9061 ctgcggcggg cccggcggtc tcgccgccga agccgccaag aatgaccgag acgaacgatc
     9121 ggttcatccc cttgcacatg atgtaggaca ggatggcgcc cgacgagccc accagcgcgc
     9181 ccgtgacgat cagtgcgatg ttgcccagcg tgaagccgat gcccgcagct gcccaaccgg
     9241 agtaggagtt gagcatagaa atcaccaccg gcatgtccgc gccgccgatc ggcacgataa
     9301 taagcacgcc gaacaaaagc gccgccgcgg tgatcgccca gaacagcacc ggatcgccgc
     9361 ccgagcggca gaaccaggcg atcagtacga ccaggagccc ggcgagcgcg gcgttgatca
     9421 ggtgccgggc cggcaggatg atcggcttgc cggacatacg tcccgataat ttggcaaagg
     9481 cgatgaccga gcccgtaaac gtcaccgcgc cgatggccac gccgagggac atctccagca
     9541 ggctggcgcc ggcgatggcg cggaaatgcc cgatgccaaa tgcttccggg gcataaagcg
     9601 cgccggcggc gacgaagaca gcggcaaggc cgacaagaga gtgaaaggcg gccaccagct
     9661 ccggcatcga ggtcatcggg atgcgccgag cgatcgtagc gccgatgccg ccgccgatac
     9721 cgagacccac caggatcagc acccacgtga acaaggaggt gtcaccgagc tgcgccaagg
     9781 tggtgacgac ggcgatggtc atgccaacca tgccgtagag gttgccacgg cgggagctct
     9841 ccgggctcga caggccccgt agcgccaaaa tgaacagcac gccggcggcg agatagagca
     9901 aggcgacgag atttggatgc atggcgcccc tcagcgcatc agcgcttcgg ctccctcttt
     9961 ttgtacatgc cgagcatgcg ctgggtgacg aggaagccgc cgaatatatt gacggaagcc
    10021 atggtcaggg cgaggaagcc aaaggtcttg gccaagccgc ttccggcttc gaccagcggc
    10081 acgccgacgg ccaggagtgc accgaccacg atcaccgagg agatggcatt ggtcaccgac
    10141 atcaggggcg tgtgcagcgc tggtgtcacg ctccacacca catagtagcc aacgaagatc
    10201 gcgatcacga acaccgtgaa ctgatagacg aaagggctga cgtccgaacc gcccacggcc
    10261 atggcgggtg cgggcaaaag cgcaaacagt aaggccagca aagccaggcc tctgccgcgt
    10321 gcagcgccag cgccgcgagt cacctgccgc agctggatct tcggatctgg ccaggccagc
    10381 gcgcgcgtca tctcatgccc cttgttgctg caggctcgga tgcacgagtt ggccgtcttt
    10441 ggccaccagc gtgcctttga tgatctcgtc atcccaattc actttcaaga ccttgtcctt
    10501 tttgtcgaac atggtctcga ggaaggcgaa caaattcttc gcgtagaggg cggaagcgtt
    10561 gacgggcacc tcgcccgcca aattcagcgg gccggcgatc gatacgcctt tatgctcgac
    10621 caccttgtcg gcttgcgtca gctcggtgtt gccgccccgc tcggccgcca agtcgacgat
    10681 gacggaaccc ggcttcatgc tctcgatcat ggctgcactg atgagcttgg gcgcaggccg
    10741 accgggaata agggccgtgg tgaccacaat gtcctgagcc ttgatgtgat tggcgatcag
    10801 ctcggcttgc ttcttttggt actcggccga catcggtttg gcgtagccgg cggcggtttg
    10861 cgcttgtttg aactcctcat cctcgaccgc gatgaacttg gcgcccagcg aggcgacctg
    10921 ctccttggtg gcgggacgca cgtcggtggc cgagacgatg gcgcccagtc ggcgcgccgt
    10981 ggcgatcgcc tgcaagcccg cgaccccgac gcccatgatg aacaccttgg cagcgggtac
    11041 cgtgcccgcg gccgtcatca tcatggggat cgcctgtcca aacatcgccg ccgcgtccac
    11101 cacggcctta tagcctgcga gatttgcctg gctcgacagc acgtccatgg actgggcgcg
    11161 cgtgatgcgc ggcataaatt ccatggacag cagtgcggcg ccggcgccgg ccaaggcctc
    11221 caggccgggt cggtcgtcga agggtgccag catggcagcg acgatggcgc cgcgcttgag
    11281 agcctcgacc tcgccgagcg atggccgcct caccttgagc agaatgtcgg ccccgctgag
    11341 cgtggccgcc gcatcggggc tgacgaccgc gccggcggcc tgcagcgctg catcggagaa
    11401 gcgggagcgc gaccccgcgc cctgttgcac gcgcacgtca gcgccgagcg cggtcagctt
    11461 cttgaccgtt tccggcgaca ccgccacgcg aggctcacct tttccctccg cagtgacggc
    11521 gatcgtgacc ataaggcttt cccctacgcg cgccggcgcg cccaagacgt aatgtgcgtg
    11581 ctgccgacta gggcaccaga tagatgtgcg tgctgccgat tagggcacca gatagaacag
    11641 catgaacagg agcagcagcg ccacccccac gatcaaggcc accgtggcct tcaggaagcc
    11701 cgcataggtt cgcaaatgct ctggatagtc catggcgggg ttcccgccgg aagtgtcgac
    11761 agccatagct cctctctcgc ttcaatgatt gcgccgcggt cttggtatag gaagccttgc
    11821 ccagcatcaa gcctcgccgg gcttgcctcg ccgggcctgg cgcgcatttc cccgcccgac
    11881 aacgaggctg ccgccgagcc tccgcatggt acgggcaagc gcgtctccga cccgccccct
    11941 tcgacccgcc ttctctccct ggggcgggca tgcttcaatg tgcgcgcgcc aacccgcacg
    12001 gcccggccaa tacgcccgca ttcccgcaga ggaggacagc catggatctc ggcatgacag
    12061 caaaggtaaa accgctggtc gaaaaagtcc gcgccatggt gcgtgacgag atcatgccac
    12121 tggaggccga atacgaggcc gaggtcggca agggcgatcg ttggacctac accaaacggc
    12181 aagaggagat ccgcgaggac ctgaaagcga aggcccgcga gcgcggcctg tggaattttt
    12241 ggctgacggg cagcgacaag ggctatggcc tgagcaccgt ggagtacgcc tatctcgccg
    12301 aggaaatggg ctggtcgcgg ttggcgccgg agaccttcaa ttgctccgct cccgatacgg
    12361 gcaacatgga ggtcatcgag cgttatggca cgccggcgca gaaagagcag tggctcaagc
    12421 ccttgttgga gggcaagatt cgctccgcct acatcatgac cgaacccaac gtcgcctcct
    12481 cggatgccac caatctggcg ctcgatgcca agctcgatgg tgaggaatgg gtgctgaacg
    12541 gcgagaagtg gtttgcctcc ggtgccggcg atccgcgctg caagatctac atcgtgatgg
    12601 tctgcagcga ccccaccgcg gacaagcaca agcggcattc tcagattttc gtgccggcgg
    12661 gcacaccggg cgttgagata ttgcggccaa tgaaggtcta tggcgacgac gacgcgccgc
    12721 acggccacat gcacgtccgt ttcacgaatg tacgcgtgcc caaggaaaac ctgatcctcg
    12781 gccgcggacg tggcttcgag gtgtcgcagg gccgcttggg tcctggccgt atccaccact
    12841 gcatgcgggc catcggtcag tcggagcggg cgctcgagct cttgtgcaag cgctctttgt
    12901 cgcgcgaagc cttcggcaag ccgttggcgc agctcggcgc caactacgac atcatcgccg
    12961 aaagccgcat gaagatcgag atggcacggc tgctctgcct caaggctgcc cacatgatgg
    13021 ataccgtcgg tcaccgcgag gcatcacctt ggatctcgca gatcaaagtc atcgcgccgc
    13081 ggatcgcctt ggaaatcacc gacgaggccg tgcaaatgca tggtgcgatg ggcatcacgc
    13141 aggacacgcc gttggcgcat atgtggacgc atctgcgcac gctcaggctg gctgacg
//
LOCUS       DASTMT010000169         2315 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_425094, whole genome shotgun sequence.
ACCESSION   DASTMT010000169 DASTMT010000000
VERSION     DASTMT010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2315
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_425094"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..241
                     /locus_tag="VFR00_03780"
     CDS             <1..241
                     /locus_tag="VFR00_03780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0158404.1"
                     /translation="GAALAQPSKREGATPTPTAVPDAQLSPTEIKERGAQYLTDCVND
                     WDKGTHMSKKDWTRTCRRVVQRRLDFMLQQGKETP"
     gene            complement(263..1609)
                     /locus_tag="VFR00_03785"
     CDS             complement(263..1609)
                     /locus_tag="VFR00_03785"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: protein motif:HMM:NF012307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase family protein"
                     /protein_id="HEU0158405.1"
                     /translation="MQPLAHLVTLVGLFLLARALLRALKRWQMRRRWRQAPQRDVSGL
                     ANSGEAAFKVEVIKTGGPLKPHHRRLVLRDNRLLPKPPPDPHRRRWPPPAKVKLLSEA
                     EADRLFGPSLRTRNRDVMDLAVDEAQLKRYGLPLWKSEADVAAALSLSRGQLRHLSQH
                     RFRETAPHYVTFAIAKRSGGERLIHAPKRRLKSVLRTLDRLLVSKLPKSEYAHGFVKG
                     RSIASNAAAHVGKAVVVHFDLKDCFPTIHFGRVRGLLLALGYSYPVAAALAVLMTEAP
                     RQPVAAEGKLYHVPTGPRVCVQGAPTSPGLCNAILARLDHRLAGLARKHGFAYTRYAD
                     DLTFSGNDTDKVGSLLAMVPRIIAAEGFLINAAKTRIFRQGGRQAVTGVIVNRTLGLS
                     RQERRKLRAALHRQKTAAAAASAGEAARLKGKLAYLFMLNRDQAAALGWQRAPKSG"
     gene            complement(1824..>2315)
                     /locus_tag="VFR00_03790"
     CDS             complement(1824..>2315)
                     /locus_tag="VFR00_03790"
                     /inference="COORDINATES: protein motif:HMM:NF016330.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HEU0158406.1"
                     /translation="YRHRELFETPADEATYFPPDRRRELAAALLSENKVRIAHVEAEE
                     NKKVRSFKDPSSGEGNSKIVREITYRDWRVIGAAGDRDAVEIVVNDLGSIIFGRCTCP
                     HFDEHLLSRGPCEHMLALFKASAELRTDRPSSTPVAAPSASAPRKQPSRWEVDDAQGE
                     TSE"
BASE COUNT          391 a          756 c          794 g          374 t
ORIGIN      
        1 tggggcggct ttggcccaac ccagcaagcg cgagggcgca accccaacgc cgacggccgt
       61 gccggacgcg caattgtccc caaccgaaat caaagaacgc ggggcgcagt atctgaccga
      121 ctgcgtcaac gactgggaca aaggtacgca catgagcaaa aaggattgga cgcgcacctg
      181 ccgtcgtgtg gtgcagcggc gcctcgactt catgctgcag cagggcaagg agacgcctta
      241 acgggcgcct cgccaacgct cctcacccgc ttttgggcgc gcgctgccaa cccagcgccg
      301 ccgcctggtc gcgattgagc atgaacaggt aggcgagttt gcccttgagg cgggccgcct
      361 caccggcgct ggcggccgcg gcggcggtct tctgccggtg cagcgccgcg cgcagcttgc
      421 gccgctcctg ccgcgacaag cccagcgtgc ggttgacgat cacgccggta acggcctggc
      481 gtccgccctg ccggaaaata cgggtcttgg ccgcgttgat gaggaagcct tcggcggcga
      541 tgatgcgcgg caccatggcg aggaggctgc cgaccttgtc cgtatcattg cccgaaaacg
      601 tcagatcgtc ggcatagcgg gtataggcaa agccgtgctt gcgggcgagg ccggcgaggc
      661 ggtgatcgag ccgcgccagg atggcattgc acagtcccgg gctggtcggc gccccctgca
      721 cgcacacgcg cggcccggtc ggtacgtgat agagcttacc ctcagccgcc accggctggc
      781 gcggggcctc cgtcatcagc accgccagcg ccgccgccac cgggtagcta tatccgagcg
      841 ccagcagcag gccccgcacc cgaccgaagt gaatggttgg gaaacagtcc ttgagatcga
      901 aatgcacgac cacggccttg ccgacatgcg ccgcggcatt gctggcaatc gagcggccct
      961 tgacgaagcc atgcgcatat tcgctcttgg gcaacttgga caccagcaga cggtcgaggg
     1021 tgcgcagcac gctcttgaga cggcgcttgg gcgcatggat caggcgttcg ccgcccgagc
     1081 gcttggcgat ggcgaaggtg acgtaatggg gcgccgtttc gcgaaagcga tgttggctca
     1141 ggtgccgcaa ctgtccccgc gacagtgaca gggcggcggc gacgtcggct tcagacttcc
     1201 acagcggcag gccgtaacgt ttgagttggg cctcgtcgac ggcgaggtcc atgacatcgc
     1261 ggttgcgcgt gcgcagcgag gggccaaaca gccggtcggc ctccgcctcg ctcaggagtt
     1321 tgaccttcgc cggcggcggc cagcggcgcc ggtgtggatc gggcggcggc ttgggaagca
     1381 gacgattgtc acgcagcacc agccggcggt ggtgcggctt cagcgggccg ccggtcttga
     1441 tgacctcgac cttgaaggcg gcctcgccgc tgttcgccag acccgagacg tcgcgctgcg
     1501 gtgcctggcg ccagcgccgc cgcatttgcc agcgtttgag cgcacgcagc agcgcgcgcg
     1561 ccaacaggaa cagccccacg agggtgacaa gatgggctag cggctgcacg gcatgcgctc
     1621 cctcgccggc caccgcattg aatgagagcg cagtcccggg ccggcaaacc aaccactaac
     1681 gaggctttgg acccgtggtc tcccctcgtt gcgtacgtgg ggaaccgtgg cgatgtgata
     1741 tggacaggaa gccaccacgg cgaagcaccg tggcatagga agctggaagg tcctgccgaa
     1801 cccgggtact gcgcaaagac gtttcattct gacgtttccc cctgcgcgtc gtcaacctcc
     1861 cagcgtgagg gctgtttgcg tggggctgag gcactggggg cggcgacggg ggtgctcgac
     1921 ggccgatcgg tccgcagctc ggcgctggct ttgaacaagg ccagcatgtg ttcgcagggc
     1981 ccgcggctca ggaggtgctc gtcgaagtgc gggcaggtgc agcgtccgaa gatgatgctg
     2041 cccaaatcgt tgacgacgat ctcgaccgca tcccggtcgc ccgccgcacc gatcacgcgc
     2101 caatcgcgat acgtgatctc gcgcacaatc ttgctgttgc cctcgccgct gctgggatcc
     2161 ttgaaggatc gcaccttctt gttttcctcc gcctccacat gcgcgatgcg caccttgttc
     2221 tcgctgagga gtgccgcggc cagctcccgg cggcgatcgg gagggaagta ggtggcctca
     2281 tcggccggcg tctcgaacag ttcgcggtgc cggta
//
LOCUS       DASTMT010000170         1903 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_3048435, whole genome shotgun sequence.
ACCESSION   DASTMT010000170 DASTMT010000000
VERSION     DASTMT010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 1903)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1903)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1903
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3048435"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..671)
                     /locus_tag="VFR00_03795"
     CDS             complement(<1..671)
                     /locus_tag="VFR00_03795"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEU0158407.1"
                     /translation="MAAARRIICVGHAALDRIYRIEAFPPEPTKVRALEHIEAGGGMA
                     ANAAVTIARLGGRAELWSRTGDDAAGASVRAGLQAERVDVRYLQAFEGARTSTSAIIV
                     DRAGERLIVGQRDAGMPSGTSWLPLERVREADAVLGDLRWLEALRSVFARAKQESIPT
                     VLDADLGAREALESILRLTDYAIFSAPALRELVPSGSDEDRLAAVRALGPGYAGVTLG
                     PEGYL"
     gene            893..1426
                     /locus_tag="VFR00_03800"
     CDS             893..1426
                     /locus_tag="VFR00_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU0158408.1"
                     /translation="MTELLARIAQRTDPAAFHELYQSYGPRVKVYLVRRGVDAATAED
                     LAQETLLTVWRRAALFSSAKGSASTWIFTIARNLWIDRLRKEVPWQELPAAHATETSP
                     EPLPDEVVAESEVQLRVRAALAELPAEQQRVIELAYLEGLSHGEIAARLGLPLGTVKS
                     RVRIAYQRLRAAVEDLK"
     gene            1423..>1903
                     /locus_tag="VFR00_03805"
     CDS             1423..>1903
                     /locus_tag="VFR00_03805"
                     /inference="COORDINATES: protein motif:HMM:NF024375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEU0158409.1"
                     /translation="MTITHHLGADTLISFAAGTLPGALSAVAAAHIALCPHCRTELVW
                     CERIGAALLEDLRAPPLATVEAAVHALPQPGAPNPARLGGNAFNPILRLARDDFADVA
                     WRRIGIGLWHRPLPLQGQGSLQLIKAAPGATVPEHSHGGGELTLILRGAMSDATGRYL
                     "
BASE COUNT          300 a          678 c          614 g          311 t
ORIGIN      
        1 cacagatagc cctcaggccc aagcgtcacc ccggcatagc cgggtcccag cgcccgcacg
       61 gcggcaaggc ggtcctcatc gctgccggac ggcaccagtt cgcgcagcgc cggcgcgctg
      121 aaaatggcat agtcggtgag ccgcagaatg ctctccaggg cttcgcgcgc cccgagatcg
      181 gcatcgagca ccgtcggtat gctctcctgc ttggcgcgcg cgaagaccga gcgcagcgcc
      241 tcgagccagc gcaggtcgcc cagcaccgca tcggcctcgc gcacgcgctc gagcggcaac
      301 cagctcgtgc ccgatggcat gccggcgtcg cgctgcccca cgatcaagcg ctccccggcc
      361 ctgtcgacga taatcgccga ggtcgacgtg cgcgcaccct cgaaggcttg cagatagcgc
      421 acatcgacgc gctcggcctg taggcccgcg cgcacgctgg cgccggcggc atcgtcgccc
      481 gtccggctcc acagctcggc cctgccgccg agcctggcga tcgtcactgc ggcgttggcg
      541 gccatgccgc caccggcttc gatgtgctct agggcgcgca ccttggtggg ctcgggcgga
      601 aacgcctcga tgcgatagat gcggtcgagc gccgcatgcc ccacacaaat gatccgcctg
      661 gccgctgcca tgccttcgtc cagcgccgtg aggccctaaa gaccccttgg aaaatgccgc
      721 ctgtgcgcac gataatgccc gctatgggcg agggaaagac caccgcgtcg gtaagtctga
      781 tccaaacccg gcccccggcc gtatcctttg ggcaacgatc ccaggattgg ctatggttgg
      841 cgattgggtg tccgtgcggg ccaggcccgg gcccggggtt agctacaacg ccatgacaga
      901 gctcctggct cggattgcgc agcggaccga ccctgccgct tttcatgagc tctatcagag
      961 ctatgggccc agggtaaaag tctaccttgt gcgccgcggc gtcgatgccg cgacggccga
     1021 ggacctcgcc caagagaccc tgctcacggt ctggcgtcgg gcagcgcttt tttccagcgc
     1081 caagggcagc gccagtacct ggatttttac catcgcccgc aatctatgga tcgatcggct
     1141 acgtaaggag gtgccatggc aggagcttcc cgcggcccac gccacggaga cctcgccgga
     1201 gcctttgccc gatgaagtcg tcgccgaaag cgaggtgcag ctgagggtgc gggcggcctt
     1261 ggccgagttg ccggccgagc agcaacgcgt gatcgaactc gcctatttgg aaggattgtc
     1321 gcacggcgaa atcgccgcac gcctgggtct gccccttggc accgtcaaat cgcgcgtgcg
     1381 cattgcctac cagaggctgc gcgccgcggt cgaggacctc aaatgacgat cacgcatcat
     1441 cttggcgccg acaccttgat cagcttcgcc gcgggcacgc tgcccggcgc cttgtccgct
     1501 gtggcggcgg cccacatcgc tctatgcccg cattgccgca ccgaactcgt ctggtgcgag
     1561 cgcatcggtg cggccttgct ggaggacctg cgggcgccac cgctggcgac cgtcgaggcc
     1621 gccgtgcacg ccttgccgca accgggcgcg cccaacccgg cgcggctcgg cggcaatgcc
     1681 ttcaatccca ttctgcggct tgcccgtgac gatttcgccg acgttgcctg gcgccgtatc
     1741 ggcatcggct tgtggcaccg gccgttaccg ttgcagggcc aaggcagctt gcagctgatc
     1801 aaggcggcgc cgggtgccac cgtccccgag cacagccatg gcggcggcga gctcaccttg
     1861 attttgcgcg gtgccatgag cgatgccacc ggccgctacc tca
//
LOCUS       DASTMT010000171         2382 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_752099, whole genome shotgun sequence.
ACCESSION   DASTMT010000171 DASTMT010000000
VERSION     DASTMT010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2382)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2382)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2382
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_752099"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..939)
                     /locus_tag="VFR00_03810"
     CDS             complement(<1..939)
                     /locus_tag="VFR00_03810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0158410.1"
                     /translation="MPLPAEMDTSSPKHLRDKYAIIGVGETSYTRGSGMTTRALGTWA
                     VRNAVEDAGLQPSEVDGMLSYQSGDSTFAPFIAGDLGIRLNFYMDVFGGGSSTEALIG
                     MAMGVIEAGMCKTVVLFRAMNGFSQVRIGGTGARSAVPVSGDMLHSRAYGWQSAGQLF
                     SFTFLRHMYDFGTTPAQVAAVRAAHSAHASNNPKAYYQKRVSVEDVLASRIICKPLHL
                     LDCCVETDNATAVIVTSADRARDGRHPRALIRGVAGRVCKPRVDMHYQHGPISTVAGK
                     YAREILWPNAGVGPADVDVTGSYDAFTFTTLLQLEDY"
     gene            complement(1069..1329)
                     /locus_tag="VFR00_03815"
     CDS             complement(1069..1329)
                     /locus_tag="VFR00_03815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0158411.1"
                     /translation="MDPQETTLTQQRTRPSEPSLRPPALLFGWLEVWLTWLATCRAYY
                     RAAIAYEELYRLPEAELRMRGYTRATLARDVTGFDVTYHGDC"
     gene            complement(1881..>2382)
                     /locus_tag="VFR00_03820"
     CDS             complement(1881..>2382)
                     /locus_tag="VFR00_03820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0158412.1"
                     /translation="DLGRYTKEETQNYVGDYVFARPSFHEDGLIYAFHMEVLWDREAS
                     ALLVREAARGKQTPAQFGKIYIPRASMHLFILSNEHGWLKKVILSQLDVYKRMKGIML
                     TMGHAFGNLYTPVAMPVIMNRYDKIDSQMLGKIERTARVYEEYNRDLVAVEEEQFAKW
                     IRIRPA"
BASE COUNT          405 a          766 c          781 g          430 t
ORIGIN      
        1 atagtcctcg agctgcagca gggtggtgaa cgtaaaagcg tcgtaggatc cggtgacatc
       61 gacgtccgcg ggtcccacac cggcgttggg ccacaagatc tcgcgtgcat atttgccggc
      121 gaccgtcgaa attggtccgt gctggtaatg catatcaacc cggggcttgc agacacggcc
      181 cgccacgccg cggatcaaag cgcgcgggtg gcgcccatcg cgcgcccggt ccgcgctggt
      241 gactatgaca gccgtggcgt tgtccgtctc tacgcagcag tcgagcaagt gcagtggctt
      301 acagatgatc cggcttgcca acacgtcctc caccgagacg cgtttttggt aataggcctt
      361 ggggttgttg gaggcgtgcg cactgtgggc tgcgcgcacc gcggccacct gcgccggggt
      421 ggtaccgaag tcgtacatgt gccgcaggaa cgtgaagcta aagagctgac ccgcgctttg
      481 ccaaccgtaa gcgcggctgt gcaacatgtc gcccgagacc ggcacagcgg acctggcacc
      541 ggtgccgccg atgcgcacct gagaaaaacc gttcatggcg cggaacaaga ccaccgtctt
      601 gcacatgcct gcctcgatca cccccattgc catgccgatc agggcttcgg tcgaggagcc
      661 gccgccgaag acatccatat agaagttgag gcggatgccg agatcgccgg cgataaacgg
      721 ggcaaaggtc gagtcgcccg attggtagga gagcatgccg tcgacctcgc tgggctggag
      781 ccctgcatcc tcgacggcat tgcgcaccgc ccaggtgccc agcgcacgcg tcgtcatccc
      841 cgatccgcgc gtatagctgg tctcgccgac gccgatgatg gcgtatttgt cgcgcaagtg
      901 cttcgggctc gatgtgtcca tctctgcggg cagcggcatg cgggcacctc ctatcgtcaa
      961 gcgccaagac tagcgcctgc ggcgacaatg ggaagcgtcc tggcaactgc gtctcaatcg
     1021 cgcgcgcagt tcgggcacgg ccggcgtgcg ggcacgcctt gctgcggctc agcagtcgcc
     1081 gtggtaggtg acgtcgaagc ccgtcacatc ccgcgccagc gtggcgcgcg tgtaaccgcg
     1141 catccgcagc tcggcctccg gcaatcgata gagttcctcg taggcgatcg cggcgcgata
     1201 ataggcgcgg caggtcgcca gccaggtcaa ccatacctca agccagccga agaggagggc
     1261 gggcgggcgc aggctgggct cgctgggtcg cgtgcgctgc tgggtcagcg tcgtctcttg
     1321 cggatccatg acatacgtcc tcgcttggga ggcattcgct cagcggcccg atcgcctcgg
     1381 cgggtcaccc ccgtcgggca ggcgcggggc ggcaacggca acggggtgcg cctcggcggg
     1441 cgccggctca cgccacggca cgacatcgga ccaggcgtgt tgcgcgaacc tttccggcct
     1501 atcggcgggc ccgagcacaa gggcttcgcc caggggggaa aacaggcaga cccgaacacg
     1561 gcgttgcgac atggatcctc ctcgcgtgcg ccagggcacg ctatcgctgt ctttttgccg
     1621 gcggccggcc ggcgatggct cggaagttct ggctccgcgc cgggggcgtg gccgccgctc
     1681 ggtgacctcg ccgcccgccc atggggccgt ggcgtcgatg ttgggagcct aagcgagcac
     1741 cgctttcgcc gcgatcgccg gagctttccg cggcttgccc aagttttccg cgttttccgg
     1801 ccgccctcag gcgagcaact gggcttgcgc ggccgaatgc ccagggattg ggatggcggt
     1861 gttctccgcc caagcccaac tcaggcgggc ctgatgcgga tccatttcgc gaactgctct
     1921 tcttccaccg ccacgaggtc gcggttgtat tcctcataaa cgcgtgcggt tcgctcgatc
     1981 ttgcccagca tctggctgtc gatcttgtcg taacggttca tgatcaccgg catcgccacg
     2041 ggcgtgtaga gattgccaaa cgcatgaccc atcgtgagca taatgccctt catgcgctta
     2101 tagacgtcga gctgggacag gatcaccttc ttcagccagc cgtgctcatt ggagaggatg
     2161 aagaggtgca tcgaggcgcg tggaatatag atctttccaa actgcgccgg tgtctgcttg
     2221 ccgcgcgccg cctcgcgcac cagcaaggct gaagcctcgc ggtcccacag cacttccata
     2281 tgaaaggcgt agatcaggcc gtcctcgtgg aaggaggggc gggcaaagac gtagtcgccc
     2341 acgtaatttt gcgtttcctc cttggtgtag cggcccaaat cc
//
LOCUS       DASTMT010000172        14611 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_3049071, whole genome shotgun sequence.
ACCESSION   DASTMT010000172 DASTMT010000000
VERSION     DASTMT010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 14611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14611
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3049071"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1632)
                     /gene="ureC"
                     /locus_tag="VFR00_03825"
     CDS             complement(<1..1632)
                     /gene="ureC"
                     /locus_tag="VFR00_03825"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018243285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit alpha"
                     /protein_id="HEU0158413.1"
                     /translation="MPMKLSRRAYADMFGPTTGDRVRLADTDLIVEVEKDLTSYGEEV
                     KFGGGKVIRDGMGQSQVSRADGAVDTVITNALIIDHTGIYKADVGLRDGRIAAIGKAG
                     NPDTQPGVDIIIGPGTEAIAGEGKILTAGGCDSHIHFICPQQIEEALNSGITTMIGGG
                     TGPATGTAATTCTPGPWHLARMLEAADAFPMNLAFAGKGNASDPSALTEMIVGGAACL
                     KLHEDWGTTPAAIDCALAVADDHDVQVMIHTDTLNESGFVEDTIAAFKGRTIHAFHTE
                     GAGGGHAPDIIKVAGLKNVLPSSTNPTRPFTVNTLEEHLDMLMVCHHLDANIPEDIAF
                     AESRIRRETIAAEDILHDLGALSMISSDSQAMGRVGEVIIRCWQTAHKMKMQRGQLKE
                     DRPNNDNFRVRRYIAKYTINPAIAQGIAHELGSLEVGKRADLVLWNPAFFGVKPDLVL
                     LGGSIAAAPMGDPNASIPTPQPVHYRPMFASYGRAVASSAVTFVSAAAAAGGLADKLG
                     LGKRLLAVKNTRGGISKKSMLLNDATPNIEVDAETY"
     gene            complement(1632..1940)
                     /locus_tag="VFR00_03830"
     CDS             complement(1632..1940)
                     /locus_tag="VFR00_03830"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011503893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0035550 - urease complex [Evidence IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit beta"
                     /protein_id="HEU0158414.1"
                     /translation="MVPGEVMCAAGDIELNAGRERISLSVANSGDRPIQVGSHYHFAE
                     SNGALKFDRDRARGYRLDIAAGTAVRFEPGQTREVNLVAFAGRRRVFGFQGAVMGELD
                     "
     gene            complement(2035..2337)
                     /locus_tag="VFR00_03835"
     CDS             complement(2035..2337)
                     /locus_tag="VFR00_03835"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008121015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit gamma"
                     /protein_id="HEU0158415.1"
                     /translation="MNLTAREKDKLLISMAALVARRRLERGVKLNYPEAVALISDFVV
                     EGARDGRSVAELMEAGAHVVSRDQVMEGIPEMIADVQVEATFPDGVKLVTVHQPIR"
     gene            complement(2353..3174)
                     /locus_tag="VFR00_03840"
     CDS             complement(2353..3174)
                     /locus_tag="VFR00_03840"
                     /inference="COORDINATES: protein motif:HMM:NF013899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreD"
                     /protein_id="HEU0158416.1"
                     /translation="MLQAMHWLPRSQGSVRLDLVNRRGATRLAVLEQSGAARVRFPKT
                     GPGEPCEAVLINTAGGLTGGDSLAIAAHLGAGANAVLTTAAAEKIYRAREDAATIVVA
                     LSVAGNAKLAWLPQPTIVFDRACLRRTTEVDLSAEASFLGLEMLIFGRAAMGESVREG
                     SLRDAWRLRRQGALLFAEALRLEGPIAEVLARPAVLDGAGATGLLLYAAPAAQERIEE
                     ARTMLHNAGATAAVSWVNGVLVARAAAPDGLSLKAQLAPLIEALHGQPLPRIWTC"
     gene            complement(3208..3903)
                     /gene="urtE"
                     /locus_tag="VFR00_03845"
     CDS             complement(3208..3903)
                     /gene="urtE"
                     /locus_tag="VFR00_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015888236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter ATP-binding subunit UrtE"
                     /protein_id="HEU0158417.1"
                     /translation="MLSVENIDLHYGAAQALRDVSLTAQPGEITCVLGRNGVGKSSLL
                     RAIMGLQPVSRGRILFDGSEITRLKTHERARLGISYVPQGREIFPLLTVRENLETGYA
                     RLPRPARKLSSELFNLFPVLNSMLGRRGGDLSGGQQQQLAIARALMMQPKLIVLDEPT
                     EGIQPSIIKDIGRAIRYLRDEGKMAIILVEQYFDFARELADTYAVMERGEVVLAGAGK
                     TMVETEVRRYLTV"
     gene            complement(3997..4767)
                     /gene="urtD"
                     /locus_tag="VFR00_03850"
     CDS             complement(3997..4767)
                     /gene="urtD"
                     /locus_tag="VFR00_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018390543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter ATP-binding protein UrtD"
                     /protein_id="HEU0158418.1"
                     /translation="MDLSIAKPHEITPSLLYLDGVSVSFDGFRALNKLSLDISPGEMR
                     AIIGPNGAGKTTMMDVVTGRTRPDSGAVLFDRGIDLTNKDEAAIAQLGIGRKFQKPTV
                     FDNHTVEDNVLLSLKGDRSVRGNLAWSSTVHEAGRIGEILHTIGLSGQRQRLAGSLSH
                     GQKQWLEIGMLIAQDPKLLLVDEPVAGMTDAETMTTAELLKDIAKNHTVIVVEHDMEF
                     VRRLGVKVTVLHEGSVLAEGSIDEVSANERVVEVYLGR"
     gene            complement(4773..6011)
                     /gene="urtC"
                     /locus_tag="VFR00_03855"
     CDS             complement(4773..6011)
                     /gene="urtC"
                     /locus_tag="VFR00_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008195045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter permease subunit UrtC"
                     /protein_id="HEU0158419.1"
                     /translation="MITRRLLQAPDGRGRIFLAILVGLAVVVPVLNLALPPASPLHVS
                     TSTVALWGKYLCYALLALSLDLVWGYCGILSLGHGAFFALGGYAMGMYLMRQIGTRGV
                     YAHPLLPDFMVFLNWPELPFYWWGFNWFAYALVMVFLVPGALAFVFGWFAFRSRVTGV
                     YLSIITQALTYALMLGFFRNNFGFGGNNGLTDFKDILGFNVQAAPTRAALFALSALAL
                     AAGYLVAQAVVRSKAGKVLVALRDEESRTRFLGYRVENYKTFVFTLSAAMAALAGALY
                     VPQVGIINPSEFAPANSIEVVIWVAVGGRGTLVGAAFGAVLVNYAKTTFTTGLLAPYW
                     LFALGGLFVLVTIFLPKGILGLLAKRAPPAPAAADWPAPRLEGAGEGPMRAGTPSGQF
                     NRDDRATARPPASGLPQPAE"
     gene            complement(6008..7609)
                     /gene="urtB"
                     /locus_tag="VFR00_03860"
     CDS             complement(6008..7609)
                     /gene="urtB"
                     /locus_tag="VFR00_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020608060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter permease subunit UrtB"
                     /protein_id="HEU0158420.1"
                     /translation="MRVSERWAAVVLGLALWLLGSLGAQAQSYESALEKFAGDSYNET
                     DAAIAGIAASGNPLAASVLEALQDARLLFGDDKRIFLRDKAGTLIDAASGKAVESTAT
                     LKPVRINNRIRRSIEAALGGLTLLAADPGKRLEAAQAVFKSKDAKALPALEQAIGKEV
                     DLGIKRALEEARAAVLIYSPTASEGEKLEAVALIRRRGDQDARGLLAGLPGDPSQSLR
                     RTVADAIAAIDNRLAAWNVVQNLWYGLSLASVLLLAAIGLAITFGVMGVINMAHGEMV
                     MLGAYTTFLVQEVIRGSAPALFDYSLAIAIPLAFLVAGLVGIAIERGVIRFLYGRPLE
                     TLLATWGISLILQQAVRTSFGPTNREVGAPHFMSGAFELGGLTITWNRLWIIVFAGII
                     FALLLAVLRFTTLGLQMRAVTQNRRMSAAMAIATGRIDALTFALGSGIAGLAGVALSQ
                     IDNVSPNLGQGYIIDSFLVVVFGGVGNLWGTLVGALSLGIANKFLEPYAGAVLGKILL
                     LVIVILFIQKRPRGLFALKGRAVEA"
     gene            complement(7682..8938)
                     /gene="urtA"
                     /locus_tag="VFR00_03865"
     CDS             complement(7682..8938)
                     /gene="urtA"
                     /locus_tag="VFR00_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009764196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033219 - urea binding [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter substrate-binding protein"
                     /protein_id="HEU0158421.1"
                     /translation="MAASVAALASLPLALSANAQDTIKVGILHSLSGTMAISETTLKD
                     VMLMLIEEQNKKGGLLGKKLEAVVVDPASNWPLFAEKARELISKEKVASVFGCWTSVS
                     RKSVLPVFKELNSILFYPVQYEGEESERNVFYTGAAPNQQAIPAVDYLMKEEQVKRWV
                     LAGTDYVYPRTTNKILEAYLKAKGVKPEDIMINYTPFGHSDWQTIVADIKKFGSAGKK
                     TAVVSTINGDANVPFYKELGNQGIAAKDIPVVAFSVGEEELAGIDTKPLVGHLAAWNY
                     FQSIKDAQNDAFIAKWKAFTKNQKRVSNDPMEAHVIGFNMWVKAVEKVKSTDPDKVID
                     ALPGIEAANLTGGVSKMLPNHHITKPVLIGEIKADGQFDVVWKTDGLVPGDAWSKELE
                     GSKDLVADWVDKKCGNFNTKTNKCGS"
     gene            complement(9171..11270)
                     /locus_tag="VFR00_03870"
     CDS             complement(9171..11270)
                     /locus_tag="VFR00_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008971171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEU0158422.1"
                     /translation="MNAPAKIAIGHSVCPHDCPSTCALDVELLGDNRIGRVHGSADHS
                     YTAGVVCAKVARYAERIHHPDRLMHPLRRKGGKGSRQFERIGWDAALDLVAEALRAAE
                     RRHGPEAVWPYYYAGTMGLVMRDGINRLRHAKRYSGMYATICTTLAWTGYIAGTGRLA
                     GPDPREMAKSDLVVIWGTNPVNTQINVMTHATRARKERGAKIVAVDIYHNGTMQQADL
                     ALCLRPGTDGALACAVMHVLFRDGYANWPYLEKYTDCPRELEAHLRARTPAWASAITG
                     LGVAEIETFARMVGTTPRTYFRLGYGFARSRNGAHNMHAALCIPSVTGAWLNEGGGGF
                     HNNGAIYHWNKSLIEGLDAVDPSIRVLDQSRIGPVLTGDPYDLKDGPPVTALFVQNMN
                     PVQVAPEQRKVKAGFAREDLFVCVHEQFMTATAKMADVVLPATMFLEHDDIYQGGGHQ
                     HIMFGPKLLEPPGECRSNHDVIQALAQRLGANHPGFAMSPRQIIDWTLQKSGWGTLAE
                     LEPNKWIDCQPDFDTAHYISGFRYPDRKFRFKPDWQNVPAPRAKAWIVERGMPSLPDH
                     WDVVEKADAEHPFRLATSPARDYLNSSFNEMPTSRARHGPPRVKVHPEDLAALGVANG
                     ERVRMGNERGEIVLPVEAFPGLRRGVVIVECIAPNDQFEGGDGINTLTSADQPGPYGG
                     AAFHDNHVWLKAAPPAR"
     gene            complement(11528..11950)
                     /gene="ndk"
                     /locus_tag="VFR00_03875"
     CDS             complement(11528..11950)
                     /gene="ndk"
                     /locus_tag="VFR00_03875"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEU0158423.1"
                     /translation="MAIERTLSILKPDATARNQTGAINALIEKAGLRIIAQKRVRWTR
                     QQAEKFYEVHKARPFYADLVRFMTSGPIVVQVLEGEAAIKAYRDVMGATDPAKAAAGS
                     IRKEFAESIERNSVHGSDSAENAQLEIGLNFKAEEIVG"
     gene            12216..13415
                     /locus_tag="VFR00_03880"
     CDS             12216..13415
                     /locus_tag="VFR00_03880"
                     /inference="COORDINATES: protein motif:HMM:NF025597.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4339 domain-containing protein"
                     /protein_id="HEU0158424.1"
                     /translation="MPAPSSQHQWYLAREGQQYGPLTDPELAKFVELGHLQPTDLLWR
                     EGFPDWRPALSVFPPSSAGAAGRKDAPAAPPAGAKPVAAPSIDGMRPGAETVDAGKRP
                     AAVAAGEDDLEPRRGFFSGRWLKRLLLIAVILAGAAFAYLWRADLGKFIGAFGTFSPS
                     SGLAATDRNGIDVPPLRGFGPDAAQMDSNFQATPLWRILKREYPEWYSERLKEIAALS
                     QQSKDDTHIAQQMGRALVTLRRQQTDNALSASLPNLKRIASTFYDNIAELRKQSTDAC
                     FAFISQGEASPMIVALLQGSPHTEHLQSQLVSVFEAAADGRKLPRVYPKPRKTDYETL
                     ATALTSLGWSQADMQLFSDERELSRAAPDKICQLVHDWFAAQLGLKDADVQLRLLVDS
                     LRPVVAG"
     gene            13590..14198
                     /locus_tag="VFR00_03885"
     CDS             13590..14198
                     /locus_tag="VFR00_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018387118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEU0158425.1"
                     /translation="MKLLSSLLSPYVRKVRIAAAMKGLVDRIEMVPIDTNIPDNPAIN
                     RANPLAKVPALLVGELAIYDSHVICEYLDGEAPAPLLFPKDKAARIKTLTLGALGDGI
                     LDAALLLVYEKRFRPQEKWHEPWQQRQQGKVDRALAGLEASPPGWEQSPDYGHLTLAC
                     ALGYLDFRHEGRWRSGHPKLVAWLDGFARAVPAFEATRPQQA"
     gene            complement(14195..>14611)
                     /locus_tag="VFR00_03890"
     CDS             complement(14195..>14611)
                     /locus_tag="VFR00_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HEU0158426.1"
                     /translation="SARAKEILEAVKRGLARDFGTLPALRSGQPLAADKVALLELLRV
                     LLKGCAAREKVAPRLIADGDDLERIAVEEHPDVAAMKGWRYEMFGRQAELLKRGALAL
                     KVEAGEVVTVELPPAARAAKEEAAPAATAARKRGTG"
BASE COUNT         2537 a         4865 c         4687 g         2522 t
ORIGIN      
        1 gtaggtttcg gcatcgactt cgatgttggg ggtggcatcg ttgagaagca tggacttctt
       61 cgagatgccg ccgcgggtgt ttttcaccgc cagcagccgc ttgccgaggc cgagtttgtc
      121 ggccaggccg ccggcggcgg ccgccgcgct gacgaaggtc accgccgagc tggccaccgc
      181 ccgcccgtag ctggcaaaca tcggccgata gtgcaccggc tgcggcgtcg ggatcgatgc
      241 gttgggatca cccatcggcg cggcggcgat gctgccgcca agcagcacca gatcgggctt
      301 aacgccaaaa aaggccgggt tccacagcac caggtcggcc cgcttgccga cctccaggga
      361 tccgagctcg tgcgcaatgc cctgcgcgat tgcgggattg atggtgtact tggcaatata
      421 gcgacgaacg cggaagttgt cgttattcgg ccgatcctcc ttcagctgac cgcgctgcat
      481 cttcattttg tgcgccgtct gccagcagcg aatgatgacc tcgcccaccc gccccatcgc
      541 ctggctgtcg gaggagatca tcgacagggc gccgagatcg tgcaggatgt cctcggccgc
      601 gatcgtctcg cgccggatgc ggctttcggc gaaggcgatg tcctcgggga tattggcatc
      661 gaggtggtgg cacaccatga gcatgtcgag atgctcttcc aaggtgttga ccgtgaacgg
      721 ccgcgtcgga ttggtcgagg acggcagcac gtttttcaac ccggccacct tgatgatatc
      781 gggcgcatgg ccgccgccgg cgccctccgt gtgaaaggcg tggatggtac ggcccttgaa
      841 ggcggcaatc gtatcctcga cgaaaccgga ctcattcagc gtatcggtgt ggatcatcac
      901 ctgcacgtcg tgatcatcgg ccaccgccag cgcgcagtcg atggcggccg gcgtggttcc
      961 ccaatcctcg tgcagcttga gacaggcggc gccgcccacg atcatttccg tgagcgcgct
     1021 cgggtcggaa gcgttgccct taccggcaaa ggccaagttc atgggaaagg catcggcggc
     1081 ctcgagcatg cgcgccaaat gccagggccc gggcgtgcag gtcgtcgccg ccgtgcccgt
     1141 ggcgggaccc gtgccgccgc cgatcatggt cgtgatgccg gaattgaggg cctcttcgat
     1201 ctgctgcgga cagatgaagt ggatatggct gtcgcagccc cccgccgtca ggatcttgcc
     1261 ttcgccggcg atcgcctcgg taccggggcc gatgatgatg tcgacgccgg gctgggtatc
     1321 cggattgccg gccttgccga tcgcggcgat gcggccgtcg cgaagtccga cgtcggcctt
     1381 gtagatgccc gtgtgatcga taatgagcgc gttggtgatg acggtgtcga cggcaccatc
     1441 ggcgcggcta acctggcttt gccccatgcc gtcgcggatc accttgccgc ccccgaactt
     1501 tacctcttcg ccgtagctgg tgaggtcctt ctcgacctcg acgatcaggt cggtatcggc
     1561 caggcgcacg cggtcaccgg tggtgggacc gaacatgtcg gcataggccc ggcgcgacag
     1621 cttcatcggc atcagtcgag ctcccccatc acggcacctt gaaagccaaa gacgcgccgt
     1681 cggccggcaa aagccaccag gttgacctcg cgggtctggc ccggttcaaa gcgcaccgcc
     1741 gtgcccgcgg caatgtccaa gcgatagccg cgcgcccgat cgcgatcgaa tttaagcgcg
     1801 ccgttgctct cggcaaaatg atagtgcgag ccgacctgga tcggccggtc gccgctattg
     1861 gcgaccgaca gcgaaatgcg ctcacgcccg gcgttgagct cgatatcgcc ggccgcgcac
     1921 atgacttcac ctggaaccac cgcccacctc ctcaagcacc agcaacacgc ggcagcgccc
     1981 gcccggcccg gcgcggagct ccgccatccc gaccgttttg cacctgccca ccgcctatct
     2041 gatcggctgg tggacggtga cgagtttgac cccgtccgga aaggttgctt ccacctgcac
     2101 gtcggcgatc atctcgggta tgccctccat gacttggtcc cggctcacca catgggcccc
     2161 ggcttccatc agctcggcga cgctgcggcc atcgcgcgcg ccctcgacca cgaaatccga
     2221 aatcagcgcc accgcctctg gatagttcag cttgacgccg cgctcgagcc ggcggcgcgc
     2281 gaccagggcg gccatggaaa tcaaaagctt gtctttttcg cgcgcagtca ggttcatgtc
     2341 ccaccccggc cttcagcatg tccagatgcg cggcagcggc tggccgtgca gcgcttcgat
     2401 cagcggcgcc agttgggcct tgaggctcag cccatccggg gccgcggcgc gcgccaccag
     2461 cacgccgttc acccagctga cagccgccgt ggcgccagca ttgtgcagca tggtgcgcgc
     2521 ttcttcgatg cgctcctgtg cagcaggcgc cgcataaagc aaaagtccgg tggctccggc
     2581 gccgtccagc acggccgggc gggccagcac ttcggcaatc ggaccttcga gccgcagcgc
     2641 ctctgcgaac agcagggcgc cctggcgccg cagccgccag gcatcgcgca ggctgccctc
     2701 gcgcacgctt tcgcccatgg cggcgcggcc aaagatcagc atctccaggc caaggaaact
     2761 tgcctcggcg ctcaagtcga cctccgtcgt gcgcctgagg caggcgcggt cgaagacgat
     2821 ggtcggctgc ggcaaccacg ccaacttggc gttgccggcg accgacaggg caaccacgat
     2881 cgtggccgca tcctcgcggg cgcgatagat cttctcggcg gcggccgtgg tcagcacagc
     2941 gttggcgcca gcaccgagat gcgctgctat ggccaggctg tcgccgcccg tcaagccgcc
     3001 ggcggtgttg atcagcacgg cctcgcaagg ctcgcccggc ccggtcttgg gaaagcgcac
     3061 gcgcgccgct ccgctttgct cgagcacggc caggcgcgtt gctccccgcc gattgaccag
     3121 gtccagccga accgatcctt ggctgcgcgg cagccagtgc atggcctgga gcacggggcg
     3181 atgatcctgt ttgcaaagct tgagactcta gaccgtaagg tagcgccgaa cctcggtctc
     3241 aaccatggtt ttcccggcgc cggcaagcac cacctcgccg cgctccatca cggcataggt
     3301 gtcggcaagc tcgcgcgcaa agtcgaagta ttgctcgacc aggatgatgg ccatcttgcc
     3361 ctcgtcgcgc agatacctga tggcgcggcc gatatccttg atgatggaag gctgaatacc
     3421 ctccgtcggt tcgtcgagca cgatcagctt cggctgcatc atcagcgcgc gcgcgatggc
     3481 caattgctgt tgctgcccac ccgaaagatc gccgccgcgc cggccaagca tggagttgag
     3541 caccggaaac agattgaaga gttcgctcga gagtttgcgc gccggccgcg gcaggcgtgc
     3601 atagcccgtt tccaggttct cgcgcaccgt cagcaggggg aaaatttccc gaccctgcgg
     3661 cacgtaggag atgccaagcc gtgcgcgctc gtgcgttttg agccgcgtga tctcgctgcc
     3721 atcgaagagg atgcggccac ggctgaccgg ctgcaaaccc atgatcgccc gcagcaggga
     3781 actcttgccg acgccgttgc gcccgagcac acaggtgatc tcgcccggtt gagccgtcaa
     3841 ggaaacgtcg cgcagggcct gcgcggctcc atagtgcaga tcgatgtttt cgacgctcag
     3901 catgcccttg caccttagct cctgcacccg cgccggccgg cgccggccaa tccggggccg
     3961 agcaagaagg ggcggtgcgc aaaggccgcc cgccacctaa cggcccaaat atacttccac
     4021 cacgcgctca ttggcgctca cctcgtcgat gctgccttcg gccagcaccg agccctcgtg
     4081 cagcacggtt accttgacgc caaggcggcg cacgaattcc atgtcgtgct ccacgacgat
     4141 caccgtatgg ttcttggcga tgtccttgag aagttcggcc gtggtcatgg tctcggcatc
     4201 ggtcatgccg gcgaccggct cgtcgaccag cagcaacttg gggtcctgcg cgatcagcat
     4261 gccgatctcg agccattgct tttggccatg cgagagcgaa ccggccaggc gctggcgctg
     4321 accgctgagg ccgatggtgt gcaagatctc gccgatgcgc cccgcctcgt gcaccgtgga
     4381 agaccacgcc aagttgccgc gcacgctgcg gtcacccttg agcgacaaca aaacgttgtc
     4441 ctccaccgta tgattgtcga acacggtggg cttttggaac ttgcgcccga tgccgagctg
     4501 ggcgatggcg gcctcatcct tgttggtgag atcgatgccc ctgtcgaaca gcaccgcgcc
     4561 gctatcgggc cgggtccgtc ccgtcaccac gtccatcatc gtcgtcttgc cggccccgtt
     4621 cggtccgatg atggcgcgca tctcaccggg cgagatatcg agcgagagct tgttgagggc
     4681 gcgaaagccg tcgaaactca cgctgacgcc gtcgaggtag agcaggctcg gcgtgatctc
     4741 gtgcggcttg gcaatagaaa gatccatggg gctcactcgg cgggctgcgg caagccgctc
     4801 gccggcgggc gagccgtggc gcggtcgtcg cggttgaatt ggccggaagg cgtgcccgcg
     4861 cgcatcgggc cctcgcccgc gccttccagt cggggcgccg gccaatcggc ggccgccggc
     4921 gccggcggcg cgcgcttggc caagaggccc agaatgccct tgggcaggaa gatcgtcacc
     4981 agcacgaaca ggccgcccag cgcgaacagc caataggggg cgagcaatcc ggtggtgaag
     5041 gtggtcttgg catagttcac caggacggcg ccgaaggcgg ccccgaccag cgtgccgcga
     5101 ccacccaccg ccacccaaat gacgacctcg atggaattgg cgggcgcgaa ctcgctcgga
     5161 ttgatgatgc caacctgcgg cacgtagagc gcgcccgcga gcgccgccat ggccgccgac
     5221 aacgtgaaca cgaaggtctt gtagttttcc acccggtagc caaggaagcg cgttcggctt
     5281 tcctcatcgc gcagcgccac cagaaccttg cccgccttgg agcgtaccac cgcctgggcc
     5341 accaggtagc ccgccgccaa cgccagagcg gacaaggcaa acaaggcggc ccgcgtcggg
     5401 gccgcctgca cgttgaaacc caggatgtcc ttgaagtcgg tgaggccgtt gttgccgccg
     5461 aagccgaagt tgttgcggaa gaagcccaac atcagggcat aggtcagcgc ctgcgtgata
     5521 atggacagat agaccccggt cacgcggctg cggaaggcga accagccaaa gacgaaggcc
     5581 agcgcgccgg gcaccagaaa caccatcacc agcgcgtagg caaaccagtt gaagccccac
     5641 caatagaacg gcagctccgg ccagttgagg aacaccatga agtcgggcag cagcgggtgg
     5701 gcatagacgc cgcgcgtccc aatctggcgc atcaagtaca tgcccatggc gtagccgccg
     5761 agggcgaaaa aggccccgtg cccgagcgag aggatgccgc aatagcccca gaccagatcg
     5821 agggacagcg ccagcagcgc atagcaaaga tacttgcccc acagggcaac cgtcgaggtg
     5881 gacacatgca aaggcgaggc gggcggcagc gccaggttga gcaccggcac caccaccgcc
     5941 agccccacca ggatggcaag aaagatgcgg ccgcgaccgt cgggagcctg caaaagccgg
     6001 cgcgtgatca tgcttccacc gcccggccct tcagcgcgaa cagcccgcgc ggccgcttct
     6061 ggatgaagag aattacgatg acgagcaaca ggatcttgcc caagacggcg ccggcataag
     6121 gctccaggaa cttgttggcg atgccgagcg acagcgcgcc gaccagggtg ccccagagat
     6181 tgccgacgcc gccgaacacg accaccagaa agctgtcgat gatgtaacct tgccccaggt
     6241 tcggggaaac gttgtcgatc tgggagagcg ccacgccggc gaggccggcg atgccggagc
     6301 ccagcgcgaa ggtgagcgcg tcgatgcgac cggtggcaat ggccatggcg gcggacatgc
     6361 gccggttctg ggtcacggcc cgcatctgca ggcccagcgt ggtaaagcgc aaaaccgcca
     6421 gcagcagcgc gaagataatg ccggcgaaca cgatgatcca aagtctattc caggtgatgg
     6481 tcaggccacc gagctcgaag gcgccggaca tgaagtgcgg ggcgccgacc tcgcgattgg
     6541 tcggcccgaa gctggtgcgc acggcctgct gcaggatcag gctgatgccc caggtggcaa
     6601 gcagggtctc gagcggacgg ccgtagagaa agcgaatgac gccgcgctcg atggcaatgc
     6661 cgaccagacc cgccaccagg aacgccaagg ggatggcgat ggccagcgaa tagtcgaaca
     6721 gtgcgggtgc cgagcctcgg atcacctcct gcacgaggaa ggtggtatag gcacccagca
     6781 tcaccatttc gccgtgcgcc atgttgatga cgcccatcac gccgaaggtg atggcaaggc
     6841 caatggcggc cagcagcagc accgatgcca gcgacaggcc ataccagagg ttctgcacca
     6901 cgttccaggc ggcgagacgg ttgtcgatgg cggcgatggc atcggcgacg gtgcgtcgca
     6961 gcgactggga cggatcgccg ggcaggcccg cgagcagacc gcgcgcatcc tgatcgccgc
     7021 gccggcggat tagcgcgacc gcctcgagct tctcgccctc gctggcggtg ggcgaataga
     7081 tgagcaccgc cgccctggcc tcctcgagcg cgcgtttgat gccaaggtct acttccttgc
     7141 cgatcgcctg ctcgagcgcc ggcagcgcct tggcatcctt ggatttgaag acggcctgcg
     7201 ccgcctccag gcgtttgccc ggatcggccg caagcagggt aaggccaccc aaggccgcct
     7261 cgatactgcg ccggatgcgg ttattgatgc gcacgggttt tagcgtcgcc gtgctctcga
     7321 ccgccttgcc gctggcggca tcgatcagcg tgccggcctt gtcgcgcaga aagatgcgct
     7381 tgtcgtcgcc gaaaagcagg cgcgcgtcct gcagggcctc gagcacgctg gccgccagtg
     7441 gattgccgct ggcggcgatg cccgcgatcg ccgcgtcggt ttcgttgtag gaatcgccgg
     7501 caaacttctc cagcgcgctt tcgtagctct gcgcctgagc cccgaggctg cccagcagcc
     7561 acagcgcaag ccccagtacg accgccgccc agcgttcgct cacccgcatg ctccaaactt
     7621 cccggtatcg aaggaggcgg cgccgatcgc cgcccccaaa cgtctcagcg ctgcagtcgc
     7681 gctaactgcc gcacttgttg gtcttggtgt tgaagttgcc gcacttcttg tcgacccaat
     7741 cggccactag atccttggag ccttccagct ccttcgacca agcatcgccg gggacgagcc
     7801 cgtccgtctt ccacaccacg tcgaactggc cgtcagcctt gatctcgccg atcaacaccg
     7861 gcttggtgat gtggtgattg ggcagcatct tcgagacgcc gccggtcaga ttggccgcct
     7921 cgatgccggg caaggcatcg atcaccttgt cgggatcggt ggacttgacc ttctcgaccg
     7981 ccttcaccca catattgaag ccgatgacgt gggcttccat cgggtcgttg ctgacgcgct
     8041 tttggttctt ggtgaaggcc ttccatttgg caatgaacgc atcgttctgc gcatccttga
     8101 tggactggaa gtagttccag gccgccaggt ggccaaccag cggcttggtg tcgatgccgg
     8161 cgagttcctc ttcacccacc gagaaggcga ccacgggaat gtccttggcg gcgatgcctt
     8221 gattgccgag ctccttatag aagggaacgt tggcgtcgcc gttgatggtg gaaaccaccg
     8281 ccgtcttctt gcccgccgag ccgaatttct tgatgtcggc gacgatcgtc tgccaatcgg
     8341 agtgcccgaa cggcgtgtag ttgatcataa tgtcttccgg cttcacgccc ttggccttga
     8401 ggtaggcttc caggatcttg ttggtggtgc gcggatagac atagtcggtc ccggcgagca
     8461 cccagcgctt tacctgctct tccttcatca ggtagtcgac ggccgggatg gcctgctgat
     8521 tgggggccgc accggtataa aagacgttgc gctcgctctc ctcaccctcg tactgcaccg
     8581 gataaaacag gatgctgttc agctccttga acaccggcaa cacggacttg cgcgacaccg
     8641 aggtccagca gccaaacacg gaggccacct tctccttgct gatcagctct cgggcctttt
     8701 cggcgaacag cggccaattc gaagcgggat cgaccacaac ggcctcgagt ttcttgccga
     8761 gcaggccacc tttcttgttt tgctcctcga tcagcatcag catcacgtcc ttcaacgtgg
     8821 tctcactgat ggccatggtg ccggaaagcg agtgcaggat gccgaccttg atcgtgtcct
     8881 gagcattggc gctgagggcc agcggcaagc ttgcgagcgc ggccacggac gccgccatcg
     8941 ctgttcgtag gattaagcgc ttcatgggtt tcaagacacc tctgttgact ggtcgagggc
     9001 tcaaccaggc cccccagagg tggttaagag caacgagcgt gccagcccgc gccggcctgc
     9061 cccaagccta ggcaattgca tggttttgcc ggccgaccgg cgcgcccgcc gcggcaaaag
     9121 gcggccacat ctgcctattt tatgggcgcg ctgcccgcga cccgatcaga tcagcgcgcc
     9181 ggcggcgccg ccttgagcca gacgtgattg tcgtgaaagg cagcgccgcc atagggtccc
     9241 ggctgatcgg cgctggtcag ggtattgatg ccatccccgc cctcgaactg atcgttgggg
     9301 gcgatgcatt ccacgatcac gacgccgcgg cgcaggcccg ggaaggcctc gaccggcagc
     9361 acgatctcgc cccgctcgtt gcccatccgc acccgttccc cgttcgccac ccctaaggca
     9421 gccagatcct ccgggtgcac cttgacccgc ggcggaccat gccgggctcg actggtcggc
     9481 atctcgttga aggaggaatt gaggtaatcg cgcgccggcg aggtggccag gcggaaggga
     9541 tgctcggcat cggccttctc caccacatcc cagtggtcgg ggagcgaggg catgccccgc
     9601 tcgacgatcc aggccttagc gcgcggggcc ggcacgtttt gccaatcggg cttgaagcgg
     9661 aatttgcggt cggggtagcg gaaccccgaa atgtagtggg cggtgtcgaa gtccggctgg
     9721 cagtcgatcc acttattggg ctcaagttcc gccagcgtcc cccagcccga tttctgcaag
     9781 gtccaatcga taatctggcg cggcgacatg gcaaagcccg gatggttggc gcccagccgc
     9841 tgcgccagcg cctggatgac gtcgtgattg gagcggcatt cgcccggcgg ctcgagcagc
     9901 ttggggccga acatgatgtg ctgatggccg ccgccctggt agatgtcgtc gtgctccaga
     9961 aacatggtgg ccggcagcac cacgtcggcc atcttggccg tcgccgtcat gaattgttcg
    10021 tgcacgcaca caaacagatc ctcacgggca aaaccggcct tcaccttgcg ctgctcggga
    10081 gcgacctgca ccgggttcat gttctgaaca aagagcgcgg tgacgggcgg cccgtccttg
    10141 agatcgtagg gatcgccggt caacaccgga ccgatgcgcg attggtcgag cacccggatt
    10201 gagggatcga cggcgtccag gccctcgatc aatgacttgt tccagtgata gatggcaccg
    10261 ttgttgtgga agccgccacc gccttcgttg agccaggcgc ccgtgacgct tgggatgcac
    10321 agggccgcgt gcatgttgtg ggcaccgttg cgcgagcggg cgaagccgta gccgagacgg
    10381 aagtaggtcc tcggcgtggt gccaaccatg cgggcgaagg tctcgatctc ggccacgccg
    10441 agcccggtga tggcgctcgc ccacgcgggt gtccgcgccc tgaggtgggc ctccagctcg
    10501 cgcggacagt cggtgtattt ttcgagataa ggccagttgg cataaccgtc gcgaaacagc
    10561 acgtgcatca cggcgcaggc gagtgcgcca tcggtgcccg ggcgcaggca cagcgccagg
    10621 tcggcctgct gcatggtgcc gttgtgatag atgtcgaccg ccacgatctt ggcaccgcgc
    10681 tccttgcggg cgcgggtggc gtgcgtcatc acgttgattt gcgtattgac gggattggtg
    10741 ccccaaatca cgaccaaatc ggacttggcc atctcgcgcg gatcggggcc ggccaggcgg
    10801 ccggtgccag cgatgtaacc ggtccaggcc aaggtcgtgc aaatggtggc atacatgccc
    10861 gaatagcgct tggcatgacg cagccgattg atgccgtcgc gcatgacgag gcccatggtg
    10921 ccggcatagt aatagggcca caccgcctcc ggaccgtggc ggcgctcggc cgcgcgcaga
    10981 gcttcggcga cgaggtcgag ggccgcatcc cagccaatgc gctcgaactg gcgtgagccc
    11041 ttgccacctt tgcgccgcag cggatgcatg aggcggtcgg ggtgatggat gcgctcggcg
    11101 tagcgggcaa ccttggcgca tacgaccccg gccgtgtagc tgtgatcggc cgagccatgc
    11161 acccggccga ttcgattgtc gcccagcaac tcgacgtcaa gcgcacaggt cgaaggacaa
    11221 tcgtgcggac acaccgaatg gccaatggca atcttggctg gggcgttcat gggcgtcctt
    11281 atccgcggcg atcccagccc ctaggctaaa cgagttcgtg gcaatggcca acaaaggggc
    11341 caagaaggcg gtcaagaaag gggccaagcg ggccgcaccg agacgacgct cggcagctcg
    11401 gccgcgaccg gctccaggcc cattgggtca aggcgcccct ggcgcgcggc cgcgtttgtc
    11461 cgcttcgcgg cttgcggcca tccagcatga cccccttgcc cctgggatgc ggccttggcc
    11521 ccgggcgtta gccgacgatc tcctcggcct tgaagttaag gccgatctcg agttgggcat
    11581 tctcggcgct gtcggagccg tgcacggaat tgcgctcgat gctttcggcg aattccttgc
    11641 gaatgctgcc ggcggcggcc ttggccggat cggtggcgcc catcacgtcg cgataggcct
    11701 tgatcgccgc ttcgccctcc agcacctgaa ccacgatcgg cccggaagtc atgaagcgga
    11761 cgaggtcggc atagaacggc cgcgccttgt gcacctcgta gaatttctcg gcctgctgcc
    11821 tggtccagcg cacccgcttt tgcgcaatga tgcgtagacc cgccttttcg atcagggcat
    11881 taatggcgcc ggtctggttg cgggccgtgg catccggctt gaggatcgac aaggtgcgct
    11941 caatcgccat cccttactcc ttgcgcgttt tttggctatt gtggcgcgcc ttatagcttg
    12001 cagccgcggc gcggttcaag cgccgatgcc caaattccgc cgcgaacggg gggcccggtt
    12061 ggcgcagggg ggctaggcct tgccttccct cggccttcgt tcgctgctaa ccccttggga
    12121 cttcgacgat tgacgccgag gaactgtgcc ccgaaactag gaaaaaatgg gaagcctcgc
    12181 cgtcccgacg gcaagccgat gctcgaatga ctgttatgcc agcgccgtcg tcgcaacatc
    12241 aatggtatct ggctcgcgag ggccagcagt atgggccgct gacggacccc gagctcgcca
    12301 agttcgtgga gctcggccac ttacagccca cggacttact ctggcgcgaa ggcttccccg
    12361 attggcgccc ggcgctttcc gtatttcccc cctcctcggc cggtgccgcc gggcgcaaag
    12421 acgccccggc agcgccgccg gcgggcgcca agccggttgc ggcgccgtcg atcgatggca
    12481 tgcgtccggg ggcggagacg gtcgatgccg gcaagcgccc cgccgcggtc gccgccggcg
    12541 aggatgatct tgagccgcgc cgcggttttt tcagcggccg ctggttgaag cggctgctgc
    12601 tgatcgccgt catcctggcg ggggccgcct ttgcctacct ctggcgcgcc gatctcggca
    12661 agttcatcgg cgccttcggc accttctcgc ccagctccgg cttggccgcg accgaccgca
    12721 atggcatcga cgtgccgccg ctgcggggct tcggccccga cgcggcgcag atggacagca
    12781 attttcaggc aacgccgctg tggcgcatcc tcaagcgcga gtaccccgag tggtacagcg
    12841 agcgcctcaa ggagatcgcc gcactcagcc agcagagcaa ggacgatacc cacattgccc
    12901 agcagatggg acgcgccttg gtcaccttgc gccggcagca aaccgacaat gcgctctcgg
    12961 ccagtctgcc gaatttgaaa cgcatcgcca gcacgtttta tgacaacatc gccgagctgc
    13021 gcaaacagag cacggacgcc tgctttgcct tcatctcgca gggtgaggcc agtcccatga
    13081 tcgtggccct gctgcagggc tccccgcaca ccgagcacct gcaatcccag ctggtgtcgg
    13141 tgttcgaggc ggccgccgat gggcgcaaac tgccgcgcgt ctacccgaag ccgcgcaaga
    13201 ccgactatga gacgctcgcc accgccttga cctcgcttgg ctggagccag gcggatatgc
    13261 agctgttctc cgatgagcgc gagctgtcgc gcgcggcgcc ggacaagatt tgccaactcg
    13321 tgcacgactg gtttgccgcc cagcttggcc tcaaggacgc cgacgttcag ctgcgcctgc
    13381 tggtcgattc cttgcgcccg gtggtggctg gctagctccc ctccaagcac gctgggcgcg
    13441 gatcgccggc acacaatccg gctgcggagg tgccagctga ttctgattgc cgctgttgcc
    13501 ggacccgggc accatccccc ctttgtttct gcacgccacc gaagccagcc gagcaaactc
    13561 agttcctcct cgcaaaaagg gcgcctgcca tgaagctgct gtcgagcctt ttgtcacctt
    13621 acgttcgcaa ggtgaggatt gccgccgcca tgaaggggtt ggtcgatcgg atcgagatgg
    13681 tcccgatcga caccaacatt cccgacaacc ccgcgatcaa tcgcgccaat cccctcgcca
    13741 aggtgccggc cctgctggtg ggcgagcttg cgatctacga cagccacgtg atctgcgaat
    13801 atctcgacgg ggaggcgcca gctcccctgc tattcccgaa ggataaagcc gcgcgcatca
    13861 aaaccctgac ccttggcgcc ctcggcgacg gcatcctcga tgccgccttg ctgctcgtct
    13921 acgaaaagcg cttccggccg caagaaaagt ggcacgaacc ctggcagcag cgccagcaag
    13981 gcaaggtcga tcgcgccctt gccgggctcg aggcgagccc gcccggctgg gagcaaagcc
    14041 ccgactacgg ccacctgacg ctcgcctgcg cgctcggcta tctggatttt cgccacgagg
    14101 gcagatggcg cagcggtcat cccaagctgg tggcctggct cgacggcttt gcccgcgccg
    14161 tgccggcctt cgaggcgacc cggccgcagc aggcttaacc ggtgccgcgc ttgcgcgcgg
    14221 cggtggcggc gggcgccgcc tcttctttgg ccgcgcgcgc cgccggcggc agctcaacgg
    14281 tgacgacctc gccagcctcc accttcaagg cgagggcgcc tcttttcagg agctcggcct
    14341 gtctgccgaa catttcgtag cgccaccctt tcatcgccgc cacatccggg tgctcctcga
    14401 cggcgatgcg ttccagatca tcgccgtcgg cgatcaggcg cggggccacc ttttcgcgtg
    14461 ccgcgcagcc cttgagcagc acgcgcaaca gttcgagcag ggccaccttg tcggcggcca
    14521 gcggctggcc cgagcgcaaa gccggcaggg taccgaaatc gcgggccagc ccgcgcttga
    14581 cggcttccag gatttccttg gcacgcgccg a
//
LOCUS       DASTMT010000173         2043 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_1733698, whole genome shotgun sequence.
ACCESSION   DASTMT010000173 DASTMT010000000
VERSION     DASTMT010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2043)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2043)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2043
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1733698"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..607
                     /locus_tag="VFR00_03895"
     CDS             <1..607
                     /locus_tag="VFR00_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007613421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0158427.1"
                     /translation="RVWWIALGYFSALYAWYAVQVHQSKYLIEIGFSPSVAAWALALV
                     SLAGIPGQIVLGHLSDRIGREWAWAAGGLGFAICYLALMAIEAYPSMGLIYLMIAAQG
                     VLGYGLTSVLGAIVLEIFQGRHYGSIFGTLMLAALAGGAAGPWLTGVLYDARGNYTLA
                     FAIGAAISVLSALAIWRAAPRKVRVVGGRLPPRALEPTNPR"
     gene            complement(594..1589)
                     /locus_tag="VFR00_03900"
     CDS             complement(594..1589)
                     /locus_tag="VFR00_03900"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015685179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003960 - NADPH:quinone reductase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase family protein"
                     /protein_id="HEU0158428.1"
                     /translation="MSGAPSYRAVVCEALGPIEGLALKRLSRAPLTPNSVRIAIKAAG
                     LNFPDILMIRGAYQHRPALPFVPGLEAAGIVTEVAPGVAVPAVGDKVIVALATGGYAE
                     EATALSAEVLPMPPAFSFAEAATFRVGHLTAYHALKTRADLKPDQTLLVLGAAGGVGL
                     ASVEIGKVLGARVIAAASGDAKLRAAQEAGADALVDYSREPVDAAVKRLTAGAGADVV
                     LDPTGFEAAAALRALAWNGKLLVVGFSAGTIPSYAANRILLKGASVVGVRAGEAGRKD
                     SRQRRQELAALTALAGAGLVRPRLSACFPLQEFAKAMRLLAERRAIGRVALLTEG"
     gene            complement(1590..>2043)
                     /locus_tag="VFR00_03905"
     CDS             complement(1590..>2043)
                     /locus_tag="VFR00_03905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0158429.1"
                     /translation="EPYTPAGIFVINEWRKIGVAAEHLQVETRTYFDSLVAGSFEVAI
                     WPLTEPADDPSAQLYGFITNKASTLSYSRHNDAKLDDLFERQQRTLDAAQRLRLVQEA
                     DRYALAQAYSVPILWWNRIIVHDKKIKGWTMSPSHFQGTDLSNVWLDP"
BASE COUNT          324 a          685 c          684 g          350 t
ORIGIN      
        1 ccgtgtctgg tggatcgcgc tcggctattt ttcggcgctc tatgcctggt atgcggtgca
       61 agtgcaccaa agcaaatatc tgatagagat cggcttttct cccagtgttg ccgcctgggc
      121 cctggccctc gtgagccttg ctggcattcc cggacaaatc gtgctcggcc acctatccga
      181 tcgcatcggc agggaatggg cctgggcagc cggcggcctc ggctttgcca tctgctacct
      241 ggcgctgatg gccatcgagg cctaccccag catggggctc atctatttga tgatcgccgc
      301 gcaaggcgtg cttggctacg gcctgacctc ggtcttgggc gccattgttc tcgagatctt
      361 tcagggcagg cattacggca gcattttcgg cacgttgatg ctggcggcgc tcgccggcgg
      421 tgcggccggc ccttggctga ccggggttct ctatgacgcc cgcggcaact atacgctggc
      481 cttcgccatt ggcgccgcca tcagcgtgct gtcggcgctg gccatctggc gcgcagcacc
      541 gcgcaaggtg cgcgtggtcg gcggccgcct accgccgcgg gcgctcgagc caactaaccc
      601 tcggtaagga gggcaacgcg cccaatcgcc cggcgctccg ccaacagacg catggccttg
      661 gcgaactctt gcaagggaaa gcaggccgac agcctgggcc tgaccaggcc ggcgcccgcc
      721 agtgccgtca gtgccgccag ttcctggcga cgctgacgcg aatccttacg gccggcttcg
      781 ccggcgcgca cgcccacgac gctcgcgccc ttgagcagga tgcgattggc cgcatagctg
      841 gggatcgtcc ccgcactgaa gcccaccacg agcagcttgc cgttccaggc gagcgcccgc
      901 agcgccgccg ccgcttcgaa gccggtggga tccaagacaa catcggcacc ggcacccgcg
      961 gtcaagcgct tgacggcagc gtcgacgggc tcgcggctgt agtcgaccag agcgtcggcg
     1021 cctgcctctt gggcggcacg caacttggca tcgccgctgg cggcggcgat cacgcgcgca
     1081 cccagcacct tgccgatctc gaccgaggcc aggccgacgc cgccggcagc gccgagcacg
     1141 agcagcgtct gatcgggctt gaggtcggcg cgcgtcttaa gcgcatggta ggcggtcaga
     1201 tgcccgaccc ggaaggtggc cgcttccgca aaggagaaag ccggcggcat cggcaacacc
     1261 tcagcgctga gggctgtggc ttcctcggcg tagccgccgg tggcaagcgc gacgatgacc
     1321 ttgtcgccga ccgccggcac ggcaaccccg ggggccactt cggtgacgat gcccgccgct
     1381 tccagccccg gtacgaacgg cagggcggga cgatgctggt aggctccccg gatcatcaaa
     1441 atgtcgggaa agttgaggcc ggccgccttg atggcaatgc gaacgctatt cggcgtgagc
     1501 ggggcacggc tcagccgctt cagcgcgagc ccctcaatgg ggcccagcgc ctcgcagacg
     1561 acggcgcgat agctgggcgc cccggacact tatgggtcga gccacacgtt gctgaggtcc
     1621 gtgccctgga agtggctagg tgacatggtc caccccttga tcttcttgtc gtgcacgata
     1681 atgcggttcc accacaggat cggcaccgag taggcctgcg ccaacgcata acggtcggcc
     1741 tcctgcacca ggcgcaaccg ctgggcggca tcgagcgtgc gctgctggcg ctcgaacagg
     1801 tcgtcgagct tggcatcgtt gtggcgcgaa tagctgagcg tggaggcctt gttggtgatg
     1861 aagccataaa gttgcgcgct cggatcgtcg gcgggctcgg tcaggggcca gatggccacc
     1921 tcgaagctgc ccgccaccag gctgtcgaag taggtccggg tttcgacctg caggtgctcg
     1981 gccgcaaccc cgatcttgcg ccattcgttg atgacgaaga tgcccgccgg cgtatagggc
     2041 tcc
//
LOCUS       DASTMT010000174         2494 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_1081546, whole genome shotgun sequence.
ACCESSION   DASTMT010000174 DASTMT010000000
VERSION     DASTMT010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2494)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2494)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2494
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1081546"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..237)
                     /locus_tag="VFR00_03910"
     CDS             complement(<1..237)
                     /locus_tag="VFR00_03910"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU0158430.1"
                     /translation="MTDRILLIEDDARLAEMVRDYLGGAGFRVARAENGGAGLAMHAR
                     EVFDAVVLDLMLPDMDGLEVCRRIRARAATPILML"
     gene            complement(247..792)
                     /locus_tag="VFR00_03915"
     CDS             complement(247..792)
                     /locus_tag="VFR00_03915"
                     /inference="COORDINATES: protein motif:HMM:NF025173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic heavy metal sensor"
                     /protein_id="HEU0158431.1"
                     /translation="MSEQSHPGEAGRRRRWPALVAVAVVAAFTGAAATGAVGQWAPGW
                     GHHGWGPGLMRGPMSPAQIEERVDRGIRHAAIELDATLEQQDKLRAIAKAAVKDLLPM
                     GEKARAARERAVELLIQPKVDRAALEAFRAEQMALADAASKRLAQAVGDAAEVLTPEQ
                     RQKAHELVRWRRGFMRGWHRG"
     gene            1079..2173
                     /locus_tag="VFR00_03920"
     CDS             1079..2173
                     /locus_tag="VFR00_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008964041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0158432.1"
                     /translation="MTRRKFVRAAGVGGVGAAAAAIAAPAIAQSAPELRWRIAASWPK
                     SLDTLYGGVEYFCRRIAEITDNKFQIQPFAAGEIVPGLQVLDAVQSGTVEMGNTALYY
                     YWGKDPALTFGTALPFGLNSRQMGAWLRFGGGHDLLNDLLKDYNCYGVAAGNTGAQMG
                     GWFRRELKSVEDLKGLKFRIGGFAGTIVSRLGVVPQQLAGGDIYPALEKGTIDACEWV
                     GPYDDERLGFQRVAKYYYYPGWWEGCGQGHNLINLAKWNELPKAYQSAISTASGETWE
                     WVLGKYDYGNPAALKRLIAGGAVLRAFPQDIMEASFRAANDIYAELSRTKPAFKKLYD
                     SVVAFRGDSFQWMQVAELGFDTFMMRHSRA"
     gene            complement(2231..>2494)
                     /locus_tag="VFR00_03925"
     CDS             complement(2231..>2494)
                     /locus_tag="VFR00_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019996297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEU0158433.1"
                     /translation="SFMHPPFGFALFYLRGIAPPSIKTSEIYWGAIPWVALQIILVAI
                     VIFWPGSVTYWLDKGTGVDPAKVRIEIPMPELPAPFEPPPKGQ"
BASE COUNT          438 a          809 c          820 g          427 t
ORIGIN      
        1 cagcatcagg atcggcgtgg ccgcgcgcgc ccggatgcgg cggcaaacct cgaggccgtc
       61 catgtcgggc agcatcagat cgaggacgac agcatcgaag acctcgcgcg cgtgcatggc
      121 gaggccggcg ccgccgttct cggcccgggc cacgcgaaac cccgctccgc cgagatagtc
      181 gcgcaccatc tcggcgaggc gggcatcgtc ctcgatcagc aggatgcggt cggtcacggc
      241 gccattctag ccgcgatgcc acccgcgcat gaaccccctg cgccagcgca cgagctcgtg
      301 cgccttctgc cgctgctccg gggtcagcac ctcggccgca tcgccgaccg cctgggccag
      361 gcgcttgctc gcggcatcgg cgagcgccat ctgctcggcg cggaacgcct cgagggcggc
      421 gcggtcgacc ttgggctgga tcaggagctc gaccgcccgt tcgcgcgccg cccgcgcctt
      481 ctcgcccatc ggcagcaggt ccttgaccgc cgccttggcg atggcgcgca gcttgtcctg
      541 ctgctccagc gtggcgtcga gctcgatggc ggcgtgcctg atgccgcggt cgacgcgctc
      601 ctcgatctgc gccgggctca tcggcccgcg catcaagccg gggccccagc cgtggtggcc
      661 ccaaccgggc gcccactgcc cgaccgcgcc ggtcgcggcg gcccctgtga aagccgccac
      721 aacggccacg gcaacgagcg ccggccatcg gcggcgcctg ccggcctctc cgggatggga
      781 ttgctcgctc atttcgctct ccttcgcttg cgatcttgcg acctgcaatc tgggaggccc
      841 cggtttcccc ctcgtctccc gattgtaaag ttttgtaact gccatccctc atcccgcgtg
      901 caaggggggc ggacccgggc ggccgtcttg tccagcgcca gggcacgcgc caggtcctgc
      961 aggcctgcat atgtcgagtt caccagatag gaaaaagcgc gtagaatgac gcatgcactg
     1021 ccgcctcgag aacgatgccg cagagcagag ttggacccag ggaggaagca tgaccaagat
     1081 caccagacgc aaatttgtcc gcgctgcggg cgttgggggg gtcggcgcgg ctgccgcggc
     1141 aattgcagcg ccggcaattg cgcaatcggc gcccgaactc aggtggcgga tcgcagccag
     1201 ctggccaaaa tcattggaca ccctctatgg cggcgtcgag tacttttgca ggcgaattgc
     1261 ggaaatcaca gacaacaaat tccagatcca gccgtttgcc gccggggaga ttgtccctgg
     1321 cctgcaggtc ctggacgccg tgcagagcgg gacggtcgag atgggcaaca cggcgctcta
     1381 ctactactgg ggcaaagacc cggcattgac tttcggaact gcgctgcctt tcggtctcaa
     1441 cagccggcaa atgggcgcct ggctgcgatt tggcggcggg cacgatctgc tcaacgatct
     1501 gctcaaggac tacaactgtt atggtgtggc ggccggcaat accggtgccc agatgggggg
     1561 ctggttccgc cgggagctca agagcgtcga agacctcaaa ggtctgaaat tccggatcgg
     1621 gggttttgcc ggcacgatcg tctcgcgcct gggtgtcgtg ccgcaacagc ttgccggcgg
     1681 agacatctat ccggcgctcg agaagggtac gatcgatgct tgcgaatggg tcggccctta
     1741 cgatgacgag aggctcgggt tccagagggt tgcgaagtac tactactatc ccgggtggtg
     1801 ggagggctgc ggccaaggcc acaacctgat caacctcgcc aagtggaacg aactgccgaa
     1861 ggcataccag tcggccatat cgaccgcgtc cggcgagaca tgggagtggg tgcttggcaa
     1921 atacgactac ggcaaccccg cggctctgaa gcgcctgatc gccggagggg ccgtattgcg
     1981 tgccttcccc caggacatca tggaggccag cttcagggcc gccaacgaca tttacgcgga
     2041 attgtcgcga accaagccgg ctttcaagaa actgtacgac agtgtggtgg ccttccgcgg
     2101 cgattccttc cagtggatgc aggtagccga gctcggcttc gacaccttca tgatgcgcca
     2161 ctcgcgcgcg taatctgcag gaccaggcgg cggccgagga gcatgcttgc tccggggctg
     2221 ccgcctgacg ctactggccc ttcggtggcg gctcgaaggg cgcgggcagt tcgggcattg
     2281 gaatttcgat tctgactttc gccggatcga cgcctgttcc cttgtcgagc cagtaggtga
     2341 ccgaccctgg ccagaagatc acgatggcca cgagaatgat ttgcagtgcc acccagggaa
     2401 tcgcccccca atagatttcc gaagtcttga tggacggagg agcgatgcct cgcagataaa
     2461 acagtgcgaa gccgaacggc gggtgcatga agct
//
LOCUS       DASTMT010000175         1871 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_95555, whole genome shotgun sequence.
ACCESSION   DASTMT010000175 DASTMT010000000
VERSION     DASTMT010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 1871)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1871)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1871
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95555"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(13..258)
                     /locus_tag="VFR00_03930"
                     /pseudo
     CDS             complement(13..258)
                     /locus_tag="VFR00_03930"
                     /inference="COORDINATES: protein motif:HMM:NF015214.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-hydroxylase C-terminal
                     domain-containing protein"
     gene            complement(498..1013)
                     /locus_tag="VFR00_03935"
     CDS             complement(498..1013)
                     /locus_tag="VFR00_03935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0158434.1"
                     /translation="MAERHAPSAVRLRPATGEDGFLLRRWLSDPATQACSGNRASAEA
                     EIALARMSETALRRIVTCEARPIGYALAMEAPAPVGAQPADLLPGTWETQLWIAPSRA
                     GVLAAIPALLAAEVFATTLAIACSGLISIRQEMMVRAYERAGFRWRRIWHDPLFGPAW
                     LMLKERPAPRA"
     gene            complement(1020..>1871)
                     /gene="purH"
                     /locus_tag="VFR00_03940"
     CDS             complement(1020..>1871)
                     /gene="purH"
                     /locus_tag="VFR00_03940"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010912183.1"
                     /note="involved in de novo purine biosynthesis; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEU0158435.1"
                     /translation="QGKELSYNNLADTDAAFELVAEFDPATAAVAIIKHANPCGVAVG
                     GSLIEAYRKALSCDPVSAFGGVIALNGAIDAATATEITGIFTEVVIAPQASAEAKAIF
                     AGKKNLRLLTTGALPDPRRQDLSFRSLSGGFLVQSRDGAMVDDLELKVVSQRPPTPQE
                     LVDLKFAFKVAKHVKSNAIVYAKGGATVGIGAGQMSRVDSARIAAWKSLEAAKAAGLS
                     EPLATGAVVASDAFFPFPDGLLAAAEAKVTAVIQPGGSMRDKEVIAAADAHGLAMVFT
                     GLRHFRH"
BASE COUNT          346 a          618 c          607 g          300 t
ORIGIN      
        1 cgggcacaag tcctatgcca ccgcactgct gtgatctcgc tgatcaacag gcgcgtcata
       61 gctacccatc agacggtcga cgatcgcttg gaacgaacac caatcggttt cgcggaaaga
      121 gtgattgcgg acgataaacg aggcaccggc gtagaacttt tcgtattgct gatgccgccc
      181 ggcaaattcg gaaccgatca gatcccacgc cagcttgaag agcttcatgc gcgtgacggc
      241 atcgcactgt ggggtctgcc aatacgtctg gaaatcactg acctatttgc cggaggccgc
      301 agaaggcgcc ggcaacatct ccaaccaggt cccgaccgac gcgagacgcc gcggcttttc
      361 aagtgcgtgt cactcccgac catttctgtt ttcgcacatc ggcgcgagac atcatgtcga
      421 agcccatcac gccccgcggg atggcatgaa ataggggaca agcgcagatg cccaagcaat
      481 ccaataaaag cgcggtgcta agcgcggggg gccggccgct ccttcagcat cagccaggcc
      541 ggcccaaaca gggggtcatg ccaaatccgc cgccagcgaa agccggcccg ctcgtaagcg
      601 cgcaccatca tctcctgcct gatggagatg aggcccgagc aggcaatggc taacgtggtg
      661 gcaaaaacct ccgccgccag cagcgccgga atggcggcaa gaacgccggc tcgcgacggg
      721 gcgatccaaa gctgggtctc ccaggtgccc ggcagcaggt ccgccggctg ggcaccgaca
      781 ggtgcggggg cttccatggc gagcgcgtag ccgatcggtc gcgcctcaca ggtgacgatg
      841 cgccgaagcg cggtctcgct catgcgcgcc agcgcaatct cggcctcggc gctggcccgg
      901 ttgccggagc aggcttgcgt cgccggatcg ctgagccagc ggcgcagcag gaagccgtct
      961 tcgcccgtcg ccgggcgcag ccgaaccgcc gatggcgcgt gccgctcggc catgcccgct
     1021 caatgccgga agtgacgaag gccggtgaag accatcgcca agccgtgcgc gtctgcggcc
     1081 gcgatcacct ccttgtcgcg catggatcca cccggttgaa tcacggccgt caccttcgcc
     1141 tcggcggccg ccagcaggcc gtctgggaac ggaaagaagg cgtccgaggc caccaccgcg
     1201 cccgtggcca gcggctcgct caggccggcc gccttggcag cctccagcga tttccaggcg
     1261 gcgatccggg cgctatcgac cctgctcatt tgcccggcgc cgatgcccac cgtggcgccg
     1321 cctttggcat agacgatggc attggatttg acgtgcttgg ccaccttgaa ggcgaacttc
     1381 aggtcgacga gctcctgagg cgtcggcggg cgttggctca cgaccttgag ttcgagatcg
     1441 tcgaccatgg cgccgtcgcg cgactgcacc aggaagccgc ccgacaacga gcggaagctc
     1501 aggtcctggc ggcgcggatc gggcagcgcg ccggtggtga gcaggcgcaa gttctttttg
     1561 ccggcaaaga tcgccttggc ctccgcgctc gcctgcggtg cgatcaccac ctcggtgaag
     1621 atgccggtga tctcggtcgc cgtggccgcg tcaatggcac cgttcagggc aatgacgccg
     1681 ccgaaggcgc tgacgggatc gcagctcagc gccttgcgat aggcctcgat cagactgccg
     1741 ccgaccgcaa caccacaggg attggcgtgc ttgataatgg cgacggcggc cgtcgcagga
     1801 tcgaattcgg ccaccagttc aaaggcggca tcggtgtcgg cgaggttgtt gtaggaaagc
     1861 tccttgccct g
//
LOCUS       DASTMT010000176         1764 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_3377178, whole genome shotgun sequence.
ACCESSION   DASTMT010000176 DASTMT010000000
VERSION     DASTMT010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 1764)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1764)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1764
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3377178"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..471)
                     /locus_tag="VFR00_03945"
     CDS             complement(<1..471)
                     /locus_tag="VFR00_03945"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Catalyzes the reduction of sulfite to sulfide, an
                     essential step in the anaerobic sulfate-respiration
                     pathway; Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite reductase subunit alpha"
                     /protein_id="HEU0158436.1"
                     /translation="MGDLPATAANALSADDSDDAPWHDAALPLAERMTLAAGRPLSQQ
                     LFAAMAQQDCGQCGYRCATYAAALDAGSESKVNLCVPGGKDTSRTLKRLLQEFAEAQA
                     PAAVAPEPVATNTVVGAAVGCGRDRPVEAIFRSATRLNAAGSAKDTHHVVLDIAG"
     gene            complement(609..>1764)
                     /locus_tag="VFR00_03950"
     CDS             complement(609..>1764)
                     /locus_tag="VFR00_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013165242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NirA family protein"
                     /protein_id="HEU0158437.1"
                     /translation="RAWHHHILNDRSLYGLPRKFNVAFDGGGIVPVLEETNDIAFTAV
                     RVRQTGEVAAGTWFRLGLGGITGHGDFARATDVIVAPSEAVAVADAIVRVFIDHGDRT
                     DRKRARLKYLLDAWGSEKFLAAVEARLGRPLGRVAPALIETSQRQQRQAHIGVHRQKQ
                     SGRIWLGVALPVGRLTTAQMRALASIAGSLGDGDIRLTVWQNLLISGVAEAEAETVER
                     RLEAIGLSARASPIRAGLVACTGNTGCKFAAANTKDTALAIAAHVEAHVALDTPLNLH
                     LTGCPNSCAQHYIGDLGLIACRVPLAPDSDDTVEGFHVHVGGGFGADAGIGRELYRNV
                     KAQDCPELVARVLQAYLANRKDRCETFLEFSRRLPLGELKALTGLAGLAA"
BASE COUNT          287 a          580 c          615 g          282 t
ORIGIN      
        1 gccagcgatg tcgagcacca cgtgatgcgt atcctttgca gagcccgcag cgttgaggcg
       61 ggtggccgag cgaaagatgg cctccacagg tcgatcccgg ccgcagccga ctgccgcgcc
      121 gaccactgtg ttggtcgcga cgggttccgg cgccacagcg gctggcgcct gcgcctcggc
      181 aaactcctgc aagaggcgct tcagcgtgcg gctcgtgtct ttgccgccag gtacgcacaa
      241 gttgactttg ctctcgctgc cggcgtccag cgccgccgcg taagttgcgc agcgatagcc
      301 gcactggcca cagtcctgct gcgccatggc ggcaaacagc tgctggctca agggacgccc
      361 cgcggccagc gtcatgcgct cggcgagcgg gagcgcggca tcatgccagg gcgcgtcgtc
      421 cgaatcgtcg gccgatagcg cgttggcggc tgtggccgga agatcgccca tatgcggcgc
      481 cgccgcagct tttgcttgag gcgcgaggag cccggcgaag aacccgttga gccaagcccg
      541 ttgttcccgc gtgaagggag cgctttccgg aatcagcgcc atggccgttg gtggcggctg
      601 ctggaccgtc atgctgccaa ccccgcaaga ccggtcaggg ctttcagctc gccaagcggc
      661 aagcggcggc tgaactcgag aaaggtttca cacctgtcct ttcggttggc gagataagcc
      721 tgcaggacgc gcgccaccag ctcggggcag tcctgcgctt tgacgttgcg atagagctcg
      781 cggccgatgc cagcgtcggc gccgaaaccg ccaccgacat gcacgtggaa gccttcgacc
      841 gtgtcatcgc tgtcgggcgc caatggcacg cggcaggcga tgagaccgag atcgccgatg
      901 tagtgctgcg cgcacgaatt gggacagccc gtcaaatgca gattgagagg cgtgtcgagt
      961 gcgacgtggg cctcgacgtg ggcggcaatc gccagcgctg tgtccttggt gttcgcggcg
     1021 gcgaatttgc accccgtgtt gcccgtgcag gccaccaggc cggcgcggat gggggaggcg
     1081 cgcgcgctga ggccgatggc ctcgaggcgg cgctcgaccg tctcggcttc cgcttcggca
     1141 accccggaaa tcaacaggtt ctgccagacc gtgaggcgga tatcgccatc ccccaggcta
     1201 ccggcgatcg atgccaaggc gcgcatttgc gctgtcgtga ggcgccccac cggcagggcg
     1261 acaccgagcc agatgcggcc ggactgcttt tggcgatgaa cgccgatgtg ggcctggcgc
     1321 tgctggcgct gggacgtctc gataagcgcc ggggcgacgc ggcccaaagg tcgcccgagc
     1381 ctcgcctcga cggctgctag gaacttctcg ctgccccagg cgtcgagcag gtacttgagg
     1441 cgagcccgct tgcggtccgt gcggtcaccg tggtcgatga acacgcgcac gatcgcatcg
     1501 gcgaccgcca ccgcctcgct gggcgcgacg atgacgtcgg tggcgcgcgc gaagtcccca
     1561 tgcccggtaa tgccaccgag ccctagccgg aaccaggtgc cagccgccac ctcgccggtt
     1621 tggcgcaccc gcacggcggt aaaagcgatg tcattggtct cctcgagcac ggggacaatg
     1681 ccgccgccgt cgaaggcgac attgaacttg cgcggcaggc cgtagagcga gcggtcgttg
     1741 agaatgtgat gatgccaagc gcgg
//
LOCUS       DASTMT010000177         6782 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_4315996, whole genome shotgun sequence.
ACCESSION   DASTMT010000177 DASTMT010000000
VERSION     DASTMT010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 6782)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6782)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6782
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4315996"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1808)
                     /gene="hyfB"
                     /locus_tag="VFR00_03955"
     CDS             complement(<1..1808)
                     /gene="hyfB"
                     /locus_tag="VFR00_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016843067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
                     /protein_id="HEU0158438.1"
                     /translation="MQVAIALWGVAVLLGTGVVATLLQGSRLATGLTYGTSFFACLGM
                     LGASCQYLLAAAASTQGLQLPLGLPWTGTHFRLDALAAFFLLVVNLGGASTSLFALGH
                     GRHETAPGRVLPFYPLFLAGMNLVVLADDAFGFLLAWEFMSVTSWALVLAHHRDPSNR
                     RAGYIYILMASFGTLALLLAFGLLAGAAGSYTFADMRAANSPLSALVLILALLGAGSK
                     AGLVPLHVWLPLAHPAAPSHVSALMSGVMTKVAVYGFIRIAFELNGPPTWWWSVLLIV
                     LAAITCVLGVLSALMQHDLKRLLAYHTVENIGIIFLGLGLCLAFSSHGLPGPAALALT
                     AALFHVFNHCLFKTLLFLGSGAVLGATGERDMEHLGGLIHRIPLTAFTFLTGCMAISA
                     LPPLNGFVSEWLTFQAILLSPALPSWALKLIIPAAGALLALSAALAAACFVKAFGVTF
                     LGRPRSDAAARAKEAEPLTLAAMVVLALLCLLAGVLPGLCIDVLAPAVESLTGARMPQ
                     QSALAYLSIVPVAASRSSYNGLMIFLFIAIAALVTIEVIHRFASRAVRRGPAWDCGFP
                     LTSPAAQYTADSFAQPIRRVFGSVIFQAREHVEMPLP"
     gene            complement(1872..2195)
                     /locus_tag="VFR00_03960"
     CDS             complement(1872..2195)
                     /locus_tag="VFR00_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013504191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU0158439.1"
                     /translation="MIIAAQLRAARALLGIDQQTLADLSGVSLPTIQRMEASQGNVRG
                     VVETLTKVVEALNAAGIELLGDNAKSDGGGRGVRFKEPAVIAQQRPAASPSRGKKGRT
                     IVRVK"
     gene            complement(2525..3451)
                     /locus_tag="VFR00_03965"
     CDS             complement(2525..3451)
                     /locus_tag="VFR00_03965"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HEU0158440.1"
                     /translation="MKAIIMDRHGGLEVLRYGDLPDPVVEPGYVVVDIHAASVNGADC
                     KVREGGSYTTVSKFPYVLGRDFSGVVGAVGAGVGDFAVGDAVFGVLAAGRDGTYAEKV
                     AEKAALLCRKPASLSHVEAAALALTGLTALVSVEDTIKLKRGETILIQGGAGGVAGYA
                     IQLAKHIGARVITTASAANHDYVMSLGADQVIDYNKEDFSKAVKDVDAVFETVGGEVA
                     GRSFAVLKPGGRAAFIASGASAPVSPRPDVQSLRPAVGRDRAHLARIVELHANKAVRV
                     PEIRLFPLREAVEAQRISEGRHFRGKLVLQVR"
     gene            3932..4774
                     /locus_tag="VFR00_03970"
     CDS             3932..4774
                     /locus_tag="VFR00_03970"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU0158441.1"
                     /translation="MAIIDSQVHAYEANTAKRPWHSVPNWPAHVTGDEMVAAMDKVGV
                     DGAIFISAFSMYRYDASYAVEVQQAHPHRFGLVKPVDPDDPAVAEVIADWKKTPGTVG
                     IRIMLPKEAQRQPSDPGLDRILRAAVRHDFPVNMLCWGNLDDGAALIDRHPDTRFILD
                     HLGIMQPRTPPAPPQPWADLPKVLELARRPNVVIKVSGACTLSREPYPFPDIWDPLAR
                     VFDAWGLERCLWGTDWTRAFAVVNYEQAVAPFLATDRLSESERGMLMGGACAKAYRWS
                     PQTA"
     gene            complement(4908..6305)
                     /locus_tag="VFR00_03975"
     CDS             complement(4908..6305)
                     /locus_tag="VFR00_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008966281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HEU0158442.1"
                     /translation="MSELWQLSATAAVDKLRSGEVSALEMVEAAAARMAAVEPSINAL
                     PIRFIDEARAQARAFRRQPTEHPGWLAGLPIAVKDYNDVAGQLTTYGSPIFAQHRPAA
                     DDRTVATLRANGAIPLAKSNVPEFAGSHTFNPVWGITRNPWDRARTAGGSSGGAAAAL
                     AAHEVWLANGSCLGGSLRIPASFCGIVGLRPSAGVVPRGDGLPAFDSLWVEGPMARGV
                     CDLALMLDAMAVSSPHDPLSRPLPPGGYQAGMRRARPPRRIGFSANLGLRRVDPEVAR
                     ICEAAVQSCKALDSAVDLAAPDFSGAIDCFQVLRALMFAGVRGELLRTERDRINPDIV
                     WNIEKGLALSAEEIIAAQRARDGLFHRVAGFFEHFDLLACPTVAVPPFAVEQRFPTEI
                     AGEKLTTYIDWMFLTFVITLTGCPAISLPCGVTKDGLPVGLQLVGRPHGDADLIGMAR
                     FLEEALAFKPLVMAA"
BASE COUNT         1297 a         2214 c         2259 g         1012 t
ORIGIN      
        1 gggagcggca tctccacgtg ttcacgcgcc tgaaaaatga cgctgccgaa cacgcgtcgg
       61 atcggttggg cgaaactgtc cgcggtgtat tgggcggcag ggctcgtcag cggaaagccg
      121 caatcccagg ccggtccgcg gcggacggcg cgcgaggcga aacgatgaat cacctcgatc
      181 gtcacgagcg cagcgatcgc gatgaagagg aagatcatga ggccattgta ggagctgcgg
      241 cttgccgcga ccggcacgat cgacaagtag gcgagcgcac tttgttgcgg catgcgtgcg
      301 ccggtgagac tttccaccgc cggggccaaa acatcgatac aaagaccggg cagcacgccc
      361 gccagcaggc agagtaaggc caagacgacc atggcggcga gcgtcaacgg ctcggcttct
      421 ttggcgcgcg ccgcggcgtc gctgcgcggg cggccgagga aggttacgcc aaaggccttg
      481 acgaagcagg ccgcggcgag cgccgccgac agtgccagca gggctcccgc ggccggaatg
      541 atcagcttca gcgcccacga cggcagggcg ggactgagca gaatggcctg aaaggtcaac
      601 cactccgaga cgaagccgtt gagcggcggc aacgcggaaa tcgccatgca acccgtgaga
      661 aaggtgaagg cggtcaacgg tatgcgatga atgagaccgc cgagatgctc catgtcgcgc
      721 tcgcccgtcg cgccgagcac cgcgcccgag ccgaggaaca agagcgtctt gaacagacaa
      781 tgattgaaaa catgaaacag cgcagcggtc agcgccagcg ccgccggccc cggcaagcca
      841 tgcgaggaaa aggccaggca aaggccgaga cccaagaaga tgatgccgat attctcgacc
      901 gtgtgatagg ccaagaggcg cttgagatca tgctgcatca gcgcgctcag cacgccgagc
      961 acgcaggtga tcgcggccaa gacgatcagc agcacgctcc accaccacgt cggcggcccg
     1021 ttgagttcaa aggcaatccg aataaagccg tagacggcaa ccttggtcat cactccgctc
     1081 atcaaggcgg agacgtggct gggggcagcg ggatgcgcga gcggcagcca gacgtgcagc
     1141 ggcaccagcc cggctttgga gcccgcaccc aacagggcaa ggatgagaac cagggcggag
     1201 agcgggctgt tggcggcgcg catgtcggca aaggtgtaac tgccagccgc acccgccagc
     1261 agtccaaagg ccaagagcag cgccagcgtg ccgaagctcg ccatcaagat atagatgtag
     1321 ccggcgcgcc tgttgctggg gtcgcgatga tgcgccagca ccagcgccca ggacgttacc
     1381 gacatgaact cccaggcgag caggaagccg aaggcatcgt cggcgagcac gaccaggttc
     1441 atgccggcga ggaacaatgg atagaagggc agcacacggc cgggcgcagt ctcgtgccgg
     1501 ccgtgcccca aggcaaagag gctggtgctg gcgccgccca gattgaccac cagaagaaag
     1561 aaggcggcga gcgcgtcgag gcgaaaatgc gtgccggtcc acggcaaacc cagtgggagc
     1621 tgcaggcctt gcgtcgaggc ggccgcggca agcaggtact ggcagctcgc ccccaacatg
     1681 ccgaggcagg caaaaaaact cgtgccgtac gtcagccccg tggctaggcg cgagccttgc
     1741 agcaacgttg caacaacgcc cgtacccagc aggacggcca ccccccacag tgcgatcgca
     1801 acctgcattc gccccctttt ccctggcgga gctgcggcgt ccgccctcac ctaaactcga
     1861 cgctcccgaa cctatttgac gcgcacgatg gtgcgtccct ttttgccgcg cgacggcgag
     1921 gccgcagggc gttgctgggc gatcacggcg ggttccttga aacgaacgcc gcggccaccg
     1981 ccgtcgctct tggcattgtc gccgagcagt tcgatgcctg ccgcattcag cgcttcgacc
     2041 accttggtca gggtctcgac gacgccgcgc acgttgccct gacttgcttc cattctttgg
     2101 atggtcggca gcgagacgcc ggagaggtcc gccagcgttt gctggtcgat gccgaggagg
     2161 gcgcgcgcgg cgcgcaattg tgcagcgatg atcatggcaa atggctgagc ccctaaaact
     2221 aggatatcaa cagtcaagcc tgatgtaaaa catatcaaga ctgaggtggc gcaagtgcgg
     2281 gcgggcggtg cttgcgcagg cgagcagaga accgcgccgt ctggcgctgg cggatgggcg
     2341 tagtccgtgc cggctcgcca cgcctcgtct ccggccggga aatgcgcggc tgcgccgctg
     2401 gccaagcgag tgcgccatcc ctggcatggg cgcgctgcgt tttagcgcct ttgggagtgg
     2461 ccggccgcaa cggcacgcct cgaaggcaaa atgccttcga gcctggcgcg ccggcgcgcg
     2521 cagatcacct cacctgcaac accagcttac cgcggaagtg ccgcccctcg ctgatccgct
     2581 gcgcctcgac ggcctcgcgc aatgggaaca gacgaatttc ggggacgcgc acggccttgt
     2641 tcgcatggag ttccacaatg cgcgcaaggt gtgcgcggtc gcgaccaacg gcggggcgca
     2701 atgactgcac gtcggggcgc ggcgaaacgg gtgcgctggc acccgaggcg ataaaagccg
     2761 cacgcccacc cggcttcagg acggcgaacg agcgcccggc gacctcgccg ccgacggtct
     2821 cgaagacggc gtcgacgtcc ttcaccgctt tgctgaagtc ttccttgttg tagtcgatca
     2881 cttggtccgc cccgaggctc atgacgtaat catgatttgc cgcgctggcg gttgtgataa
     2941 cgcgagcacc gatgtgtttg gcgagctgga tggcgtagcc cgccacaccg ccggccccac
     3001 cttgaatgag aattgtctca ccacgcttga gcttgatcgt gtcctcgacc gaaaccagcg
     3061 ccgtcaagcc agtgagcgcg agcgcagcgg cctcgacgtg cgaaagggag gcgggcttgc
     3121 gacaaagcaa ggcggctttc tccgcgacct tctcggcata ggttccatcg cgtccggcgg
     3181 caagcacgcc gaacacggca tcgccaacgg cgaagtcgcc gacgccggcg ccgacggccc
     3241 ccaccacgcc ggaaaaatcg cgaccgagca catagggaaa tttggagacg gtggtgtagg
     3301 aaccaccttc gcgcaccttg caatccgcac cattgacgct cgcggcgtgg atgtcgacga
     3361 cgacgtaacc cggctccacc acgggatccg gcaggtcgcc atagcgcaga acctcgagac
     3421 cgccgtggcg gtccatgatg atggccttca tctggtcgtt cctgcccgtg cgcaaggccg
     3481 cacctttgcg gcaccttggt tgcgatgggg gaacttgcac cgcaatcgtg cccgaagcaa
     3541 cgggccggcc gtcaagctgg ttcggcaaac tcgtctcccc agcgcgcgca gacctggggc
     3601 cgctgtcgtc cggggctcgg cgtcgcgagg ctaacggggt tcacccgcga gccccggagg
     3661 acaacagatg tttcaaagtg ttatctcccg catgggccaa gggcctttgc tgggacaagc
     3721 ccagtgcctt tgccgcgcgc gtccactgca ggcgcctggc acgtccgccg gcatgccagc
     3781 gcgcgctggg atcgatcagc gccggcccga catgagggcg gcgagcagcg gcggcagagt
     3841 acgccgagcc gcgcgccggc gccgagcgtc tggacatggc gggcgcgacc tgctgccata
     3901 ccaaggcgcc tagcacagag gagcatcccc aatggcaatc attgattccc aagtccatgc
     3961 ctatgaggcg aataccgcca agcgaccctg gcatagcgtg ccgaactggc ccgcgcacgt
     4021 gaccggagac gagatggtgg cagcgatgga caaggtcggc gtggacggcg caatctttat
     4081 ctccgccttc tccatgtatc gctacgacgc cagctatgcg gtggaagtgc aacaggccca
     4141 tccccatcgg ttcggcctcg tcaaaccggt cgatccggac gatccggcgg tggccgaggt
     4201 cattgccgat tggaagaaga cgccgggcac cgtcgggatc cgcatcatgc tgccgaagga
     4261 ggcgcagcgg cagccgagcg acccgggcct cgaccgcatc ctgcgcgccg ccgttcgcca
     4321 cgactttccc gtcaatatgc tgtgctgggg caacctcgac gacggcgcgg cgctgatcga
     4381 tcggcatccc gacacgcggt ttatccttga tcatctcggc atcatgcagc cgcgcacgcc
     4441 gccggcccca ccacagccct gggccgattt gccgaaggtg ctggagctcg ccaggcgccc
     4501 caacgtggtg atcaaagtca gcggcgcctg cacgctctcc cgcgaaccct atcccttccc
     4561 cgacatctgg gacccgctcg cccgcgtctt tgatgcctgg ggcttggagc gctgcttgtg
     4621 ggggaccgac tggacgcgcg ccttcgccgt tgtcaactat gagcaggccg ttgcgccatt
     4681 tcttgccacg gaccggctga gcgaaagcga gcggggcatg ctgatgggcg gcgcctgcgc
     4741 caaggcctat cgctggtcac cgcagacagc ctaggtgttc gcccagcagg acttcgccga
     4801 gcgcctcgtt tgccgcgtct gcggtgaggg caacgccgcg ccggctgggc gagcgagacg
     4861 gtgatgctgg cgaaggcggc gcaagctttg ggggcgctgg gcgcccccta ggccgccatc
     4921 accaagggct tgaaggcgag cgcctcttcc aggaaccttg ccatgccgat gagatcggcg
     4981 tcgccgtgcg ggcggccgac gagctggagg ccaacgggaa ggccgtcctt ggtcacgccg
     5041 cagggcaaag agatggcggg acagccggtg agggtgatga caaaggtcaa aaacatccag
     5101 tcgatgtaag tggtgagctt ctcgcccgca atctcggtgg gaaagcgctg ctccaccgcg
     5161 aatggtggta ctgccacggt cggacatgcc agcaagtcga agtgctcaaa aaagcctgcc
     5221 acgcgatgaa acagcccatc gcgcgcacgc tgagcggcga taatctcctc ggccgagaga
     5281 gccaatcctt tctcgatgtt ccagacgata tcggggttga tgcggtctcg ctccgtccga
     5341 agcagctcac cgcggacgcc tgcaaacatg agggccctca gcacttggaa gcaatcgatg
     5401 gcgccggaaa aatccggtgc agccaagtcc accgcgctgt ccagcgcctt gcagctctgc
     5461 accgccgctt cgcagatgcg ggcaacttcc ggatcgaccc tgcgcaagcc gagatttgcg
     5521 ctgaagccga tgcgtcgagg ggggcgtgcg cgccgcatgc ccgcctggta gccgccgggc
     5581 gggagcggtc gcgacagcgg atcgtgcggg ctcgataccg ccatggcatc gagcatgagg
     5641 gcgagatcgc agaccccgcg cgccatcggg ccttccaccc aaaggctgtc aaatgcaggt
     5701 aagccgtcgc ccctcggcac gacgccggcg ctcggccgca agccgacgat gccgcaaaag
     5761 ctcgccggaa tgcgcagtga acccccaagg caggaaccat tcgccagcca aacttcgtgg
     5821 gcagcgagcg cggccgcagc gcctcccgat gagccgcccg cggtgcgcgc gcgatcccag
     5881 ggattgcggg tgatgcccca caccgggttg aacgtgtggc tgcctgcgaa ctccggcacg
     5941 ttcgattttg ccagcgggat ggcgccgttg gcgcggagcg tggcaaccgt cctgtcgtcc
     6001 gccgctgggc gatgctgggc aaagatcggc gagccatagg tcgtcagctg ccccgcgacg
     6061 tcgttgtagt ccttgaccgc gatcggcaag ccggcgagcc agccgggatg ctcggtgggc
     6121 tggcgccgga acgctctcgc ctgcgctcgg gcctcgtcga taaaacggat gggcaaggcg
     6181 ttaatagagg gctcaacggc agccatgcgg gcggccgcag cctccaccat ctccagcgcc
     6241 gatacctcgc cgctgcgcag cttgtcgacg gcggcggtgg ccgaaagctg ccaaagctcg
     6301 ctcatccttg catcctcagg ctgttgtcgc cggcaggcga tggcaaatgc gccgtgcata
     6361 aaaccggggc gggaaagcgc gtgcgcggca agctcgcgtg cgaagagagc atcgaagcct
     6421 ctggatcctt tgacgcaccc tccgtctgtg tagacgatcc gaggctgacg gcgcaacgcg
     6481 cagccccgtc ggtgaaacca ggctcggcag ccggccgggg cgtcgcgaag accgccggtg
     6541 cttattggag gttagggagg ctcatccctt ggcggaggcc agtttttgcc tgcgcccacc
     6601 gatttcgctc cgaacggaag gcaaaccgct tcagcccgcc catagccggt tcgcggcagg
     6661 gtaggcgaca ccaagggttc acgcctgcgc agcagccgag cgccaggccc ggccgatcca
     6721 acgggcaaga tgcgccgcaa gcatcgacgg ccagcagtga ttcatgccgg cgtaggccag
     6781 cg
//
LOCUS       DASTMT010000178         1811 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_96486, whole genome shotgun sequence.
ACCESSION   DASTMT010000178 DASTMT010000000
VERSION     DASTMT010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 1811)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1811)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1811
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_96486"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1811)
                     /locus_tag="VFR00_03980"
     CDS             complement(<1..>1811)
                     /locus_tag="VFR00_03980"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEU0158443.1"
                     /translation="TGAKVAPSAAAKVAAPAASSLPPVARPTLLPGESEHVARYDAAI
                     APLRNLTLAEDDAALLREAIGAGANGKLAEAKAVRDRIKEQAARKLVDWFIFRAGYGG
                     AMEIKAFLEANPAWPDRNLLNQRAEEALFNSASASSRDIKAFFAAAEPRTGVGQAALA
                     AAFLADKDNDRAKAIAVKAWTEYDIPASLEPAFLKRVGPLLSEADHKRRLNRLLLNDS
                     RWTNERNERAAIIRRMLPLLSEPEKKAAEARLAVFLRAKNSQTLIAKLPTSTAPDWGL
                     AVQKAQALRRQNKEEEAWKILLTEPEAPGTTKPDGWWEERRANAYAALRLGKPKMAYE
                     LIREPGPLSVNAAKDAAFLAGWLNLRYLDEPKQALAHFQTLAGMADGPLSRARASYWL
                     GRTYEVLGDKAKALEQYRAGAVYFDTFHGQLARLKVDPGANRLSLALPSAPTPEEIAR
                     FTSSDAVRAIVVARKAGLDAALSRAFLRHLPGYLKSEAEVAMLAHLADALGDAQMSVR
                     VGKAAIARGMNLAYYAYPVRALPQYTPLRRPPEPAFILGIARQESEFNTLTLSGAGAR
                     GILQVMPVTAHHVCRDYKLKCDIPRLMKDPVYNTMMG"
BASE COUNT          265 a          588 c          656 g          302 t
ORIGIN      
        1 tgcccatcat ggtgttgtag acgggatcct tcatcagccg cggaatgtcg cacttgagct
       61 tgtagtcgcg gcagacgtgg tgagcggtga cgggcatgac ctgcaggatg ccgcgggcgc
      121 cggccccgga cagcgtcaag gtgttgaact cgctttcctg ccgggcgatg cccaggatga
      181 aggcgggctc gggcggtcgg cgcagcgggg tgtattgcgg cagcgcccgc acggggtagg
      241 cgtagtaggc gaggttcatg ccgcgggcga tggcggcctt gccgacgcgg accgacattt
      301 gcgcatcgcc gagggcgtcg gcgaggtggg ccagcatcgc cacctcggcc tcgcttttca
      361 aatagccggg cagatgccgc aggaaggcgc gggacagcgc cgcgtcgagc ccggccttgc
      421 gcgccacgac gatggcgcgg accgcatcgg aactggtgaa gcgggcgatt tcctccggcg
      481 tcggcgccga cggtagggca aggctcagcc gattggcgcc gggatcgacc ttgagacgcg
      541 ccagctggcc gtggaaggtg tcgaaataaa cggcgccggc gcgatactgc tcgagcgcct
      601 tggccttgtc gcccaggacc tcataggtgc ggccgagcca atagctggcg cgcgcccggc
      661 tcaaggggcc gtcggccatg ccggcgagcg tctggaaatg ggccaaggcc tgcttgggct
      721 catcgagata gcgcaagttc aaccagcccg ccaaaaaggc ggcatccttg gcggcgttga
      781 cggataaggg acccggctcg cggatgagct cgtaggccat tttgggcttg cccaggcgca
      841 gcgcggcgta ggcgttggcc cgccgctcct cccaccagcc gtcgggcttg gtcgtgcccg
      901 gtgcctccgg ctcggtcaag aggatcttcc aggcctcctc ttctttgttc tgccggcgca
      961 gggcctgcgc cttctgcacc gcgaggcccc aatcgggcgc cgtcgaggtc ggcagtttgg
     1021 cgatcagcgt ttgcgaattc ttggcgcgca agaacacggc gagacgggct tccgccgcct
     1081 tcttctcggg ctccgacagc agcggcagca tgcgccggat gatggcggcg cgctcgttgc
     1141 gctcgttggt ccaacgactg tcgttgagca ggagccgatt gagccggcgc ttgtggtcgg
     1201 cctcgctcaa cagcggcccg acgcgcttga gaaaggccgg ctccaggctg gcgggaatgt
     1261 cgtactccgt ccacgccttg accgcgatcg ccttggcgcg atcgttgtcc ttgtcggcca
     1321 gaaaggcggc cgcgagcgcg gcctggccaa ccccggtcct gggctccgcc gcggcgaaga
     1381 aggccttgat gtcgcgagag cttgccgagg cagaattgaa cagggcctcc tcggcgcgct
     1441 ggttcagaag gttgcggtcg ggccaggccg gattggcctc cagaaacgct ttgatctcca
     1501 tggcgccgcc gtagccggcg cgaaagatga accagtcgac gagcttgcgc gcggcctgct
     1561 ccttgatgcg gtcgcgcacc gccttggcct cggccagctt gccgttggcg cccgccccaa
     1621 tggcttcgcg cagcaaggcg gcatcgtcct cggccagcgt caagttgcgc aacggggcga
     1681 tggccgcatc gtagcgggcg acgtgttcgc tctcgcccgg cagcagcgtc ggccgcgcca
     1741 cgggcggcag cgacgaggcg gccggtgcgg ccaccttggc ggccgcgctc ggcgcaacct
     1801 tggcaccggt t
//
LOCUS       DASTMT010000179         2511 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_5301214, whole genome shotgun sequence.
ACCESSION   DASTMT010000179 DASTMT010000000
VERSION     DASTMT010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2511
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5301214"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..427)
                     /locus_tag="VFR00_03985"
     CDS             complement(<1..427)
                     /locus_tag="VFR00_03985"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEU0158444.1"
                     /translation="MTKAWHKGLARLARSLTPLAAAVALIAAVSASSAQERSRLDEVL
                     QRGSLIVVTLGTVPPFAFKDDKGELVGFDIDVARLAANALFRDPNKIEFVVVTSDGRW
                     PAIESGRADMGVGGTTVYPDRAIRVAFTRAFIDSGISIMV"
     gene            complement(452..1723)
                     /locus_tag="VFR00_03990"
     CDS             complement(452..1723)
                     /locus_tag="VFR00_03990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0158445.1"
                     /translation="MGMDRFGNQHAPNLSYARGAILASTADDFRKLQQAWRLMRERGP
                     ESIFIFTGLEHSLPMQQEDLLFADDEVGPSLHFERLKALALEHLGGSADEHDVAPFNR
                     LTGATMATHLTLVKPGDAVIGVSASHSHPSVIRAANHVGSRFTDTAGLAAFKEALQRE
                     PKVSLVVLTRLAVTYDLLPLESIATIVRLAHAKGALVYVDDAGGARVGPAAFDQPRML
                     ELGVDVGATGLDKYGTVGPRFGLLAGRKELVERIRAKGFEFGLECRQMLYPAVVRTLE
                     QYSPQRVRDLIAATQRVSAELRPMIGNRLRETPTTAQISADDILEIAMERGELKKAPI
                     VPFEATAALCMLLLRDYNMLTVHFVGVPPGGADLLIKFLPPETLARFGGPKKFAQALD
                     ASLTKLGKMLRKPESLRELLLGTNAETVVRG"
     gene            2394..>2511
                     /locus_tag="VFR00_03995"
     CDS             2394..>2511
                     /locus_tag="VFR00_03995"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020186333.1"
                     /note="catalyzes the formation of phosphonoacetaldehyde
                     from 2-aminoethylphosphonate and pyruvate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HEU0158446.1"
                     /translation="MAEAFVRGASETGDPLLLTPGPLTTSLSVKRAMLHDWGS"
BASE COUNT          445 a          812 c          816 g          438 t
ORIGIN      
        1 ccaccatgat cgaaatgccc gaatcgatga aggctctggt aaaggccacg cggatggcac
       61 ggtccggata gacggtggtg ccccccacgc ccatgtccgc ccggccgctt tcaatggcgg
      121 gccagcgacc atcgctggtg accacgacaa attcgatttt gttggggtcg cggaagagcg
      181 cattggcggc gagccgcgcc acgtcgatgt cgaagccgac gagctcgccc ttgtcatcct
      241 tgaaggcaaa cggcggtacc gtgccgagcg tcaccacgat cagtgagccc cgctgcagga
      301 cttcgtccag gcgcgagcgc tcctgggcgc tgctggcgct caccgcagcg attagagcca
      361 cggcggcggc cagcggggtc agcgatcttg ccaggcgagc caagcctttg tgccaagctt
      421 tcgtcatcac tgcctcccat taagaaagca cctagccgcg caccaccgtc tcagcgtttg
      481 tgcccagcaa cagctcccgt aggctctcgg gcttgcgcag cattttgccc agtttggtca
      541 gcgaggcgtc aagcgcttgc gcaaattttt tgggcccgcc gaagcgtgcc agcgtctcgg
      601 gcggcaagaa cttgatcaac aggtccgcgc cacccggcgg cacgccgaca aagtgcacgg
      661 tgagcatgtt gtagtcgcgc aaaagcagca tacacagcgc cgcggtcgcc tcgaagggca
      721 cgatcggcgc ttttttcagt tccccgcgct ccatggcgat ttccaggatg tcgtcggcgc
      781 tgatctgcgc cgtggtcggg gtctcgcgca gacggttgcc gatcatgggc cgcaactccg
      841 cgctgacgcg ctgggtggcc gcgatcaggt cgcgcacgcg ttgcggacta tactgctcaa
      901 gggtgcgcac cactgccgga taaagcatct gccggcactc gaggccaaac tcgaagccct
      961 tggctcgaat gcgctcgacc agctccttgc gcccggcgag caagccaaag cgcggtccca
     1021 cggtgccata tttatcgagg ccggtggcgc cgacatcgac cccgagctcc aacatgcgcg
     1081 gctgatcgaa ggcggccgga ccaacgcggg cgccacccgc atcgtccaca tagacaaggg
     1141 ccccctttgc atgcgccaag cggacaatgg tggcgatgct ctcgagcggc aacaggtcgt
     1201 aggtaacggc gagccgcgtc agcaccacta gggaaacctt tggctcgcgc tgcaaggcct
     1261 ccttgaaggc ggcaagaccg gcggtatcgg taaagcggga gcccacgtga ttggcagccc
     1321 tgatcaccga gggatgactg tggctagcgg agacgccgat taccgcgtcg ccgggtttga
     1381 cgagggtcag gtgggtcgcc atggtcgccc cggtcaggcg gttgaagggg gcgacatcgt
     1441 gctcgtcggc agagccgccc aagtgctcga gcgccagcgc cttcaagcgc tcgaagtgga
     1501 gcgagggccc gacctcgtcg tcggcgaata acagatcctc ctgttgcatc ggcagggagt
     1561 gctcgaggcc cgtaaaaata aaaatacttt ccgggccacg ttcgcgcatg aggcgccagg
     1621 cctgttgcag tttgcggaaa tcatcggcgg tgctggccag gatggcgccg cgcgcatagg
     1681 agagattggg ggcatgctga ttaccaaatc gatccattcc catgcttgca tgctcctcgt
     1741 tcggcctcgg caatggctgg ctggagcgcc gtgccgaagt gcttgagccc acggtcgcga
     1801 ccgctctgcg aaatgtcatt tgtataggag accgtacctt agcgccgact cgagcgtcaa
     1861 gggcgaccag cgccgtggcg cagcgcacag ggccgcgcga agccttcgcg gcagggctgc
     1921 tccggtgtgg catagacatt tcactggctt ggcttagcgc acggcgaacc gcccgcgctt
     1981 gggggccttt tttatgccgc gcgcgcgaca ccaacttcct cgtcgaggcg atcgccgatt
     2041 ggctcgaaag cgacggcagc gatgatgccc gctggtgatc ggcgccgcct acccccgcat
     2101 ttgagcaagg ggtcggtcgc tataagttct ttttctaact gtccttcaag cccaagtttt
     2161 aggcacgcct gcggcccagg attcctgggc catgacacac gggattggcg cgggaggccc
     2221 gcggccgccc cgccggggca cagcgaccgg ctcgcttggc ttggccggcc agaagacggc
     2281 gggcaccccg ccgcgcgcgc gcgccccgca tgcttccgtt tgcttaagct cgtgcggacg
     2341 tctagccttc gcttggcggg cagcgggaaa cgccgaaacg taagggacga gccatggccg
     2401 aagcctttgt tcgcggcgcg tccgaaaccg gcgatccgct cctgttgacc cccgggcccc
     2461 tcaccacgtc cttgagcgtg aagcgggcga tgctgcacga ctggggctcg c
//
LOCUS       DASTMT010000180         7234 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_5957141, whole genome shotgun sequence.
ACCESSION   DASTMT010000180 DASTMT010000000
VERSION     DASTMT010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 7234)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7234)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7234
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5957141"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..307
                     /locus_tag="VFR00_04000"
     CDS             <1..307
                     /locus_tag="VFR00_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016846985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0158447.1"
                     /translation="ALIIGAFALAGFLSGIIMPSRDMLVRKAAPPGMVGRAFGIVSTG
                     FNIGGIIGPLLFGWIMDNASPRWVFGACAVFMAVTALFGIASEARARRRLAAQVSAR"
     gene            601..1893
                     /gene="murA"
                     /locus_tag="VFR00_04005"
     CDS             601..1893
                     /gene="murA"
                     /locus_tag="VFR00_04005"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEU0158448.1"
                     /translation="MDRINIVGGRRLKGTIPISGAKNAALPLMIASLLTGDRLTLKNV
                     PNLADVNLLARILRNHGVDLTIDGKRAGPSPYLGETFHLTARDIVDTTAPYEMVNRMR
                     ASFWVLGPLLARCGRARVSMPGGCAIGTRPVDLHLFGLKALGASIDIDGGYVVAKAPK
                     RLKGARVSFPKVSVGATHTVLMSAALADGETVIENAAREPEVGDVAECLVKMGARIEG
                     IGSKTLRIEGRERLEGAVHTVLPDRIETGTYAFAVAAAGGDVELKGARKGLLEAAFAT
                     LEATGVSVADTADGVRIARDSGELEPIRIETQPFPGFPTDLQAQLMALMTRAKGNSVI
                     RETIFENRFMHVQELVRLGADIQLQGDTAMIRGVPKLQGAQVMATDLRASVSLVIAGL
                     MAEGETIINRVYHLDRGFERLEQKLSGCGASIARLVSG"
     gene            2062..2535
                     /locus_tag="VFR00_04010"
     CDS             2062..2535
                     /locus_tag="VFR00_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015931414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2948 family protein"
                     /protein_id="HEU0158449.1"
                     /translation="MAELKLIALDADDLAVLSAHLQDAVIQVADIAYLPKERRFAAIG
                     NRFDWMAAADAGVGYARRRAAVRFERVLGAQVQAIDLKNKASVLSLLAVTFEPTTAPE
                     GYVTLHFADGGAIRLHVECVEAELKDLGPIWRVDFKPQHPDGSPVSPSPGKERST"
     gene            2557..3864
                     /gene="hisD"
                     /locus_tag="VFR00_04015"
     CDS             2557..3864
                     /gene="hisD"
                     /locus_tag="VFR00_04015"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019962916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HEU0158450.1"
                     /translation="MPIQLASTDVNFEAAFASFLASKREVSPDVDERVRAIIADVRTR
                     GDAALIEYTRRFDQLDLASVGIRLDEAAIRAARRDCDPATLDALLLAKARIEAYHLRQ
                     VPKDESFRDPVGAELGWRYSPIESVGLYVPGGLASYPSSLLMNAVPAKVAGVPRIAIA
                     VPAPRGELNPFVLAAADIAGVTEIYRMGGAQAVAAFAYGTQSVPQVAKIVGPGNAYVA
                     AAKRQVFGVVGIDAIAGPSEVLIIADAASDPVWLAADLLAQAEHDTAAQAILMTDDAT
                     LAARVEAEVERQLGLLPRRDIAAASWREFGALIVLESLEQAPALADRIAAEHVQILTR
                     DPDRLAGQIRNAGAVFLGAHTPEVIGDYVAGSNHVLPTARSARFASGLGVQDFLKRTS
                     TLRLDAAALSRLAAPAMVLARAEGLTAHERSVAVRLGLSAERS"
     gene            3872..4369
                     /locus_tag="VFR00_04020"
     CDS             3872..4369
                     /locus_tag="VFR00_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012113252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0262 family protein"
                     /protein_id="HEU0158451.1"
                     /translation="MFEPPASDANPKARLTAITLDSTSIKRATSGIEHEREVAIYDIL
                     ESNFFSLEGRDEGPYTLNLSIAEDRLVLAVGQAAGDSVFTVVLSLTPLRRVMKDYFIV
                     CESYYRAIRTAPPSRIQSIDMGRRALHDEGSRVLLERLKGKISVDNDTARRLFTLICA
                     LHWKG"
     gene            4373..4837
                     /locus_tag="VFR00_04025"
     CDS             4373..4837
                     /locus_tag="VFR00_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013421081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphatase family protein"
                     /protein_id="HEU0158452.1"
                     /translation="MAGAGGQELPGAVLFACTMNSVRSPMAAAILRHLAGRRAYVESA
                     GVRAGGLDAFAVAAMEEIGIDISQHAPRTLRELNDTSFDLIISLSPEAHHQALELTRT
                     MAVDVEYWPTFDATLLIGSASREQILQGYREVRDQLFQRIKRRFGFEGGPSV"
     gene            4923..5669
                     /locus_tag="VFR00_04030"
     CDS             4923..5669
                     /locus_tag="VFR00_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf-like protein"
                     /protein_id="HEU0158453.1"
                     /translation="MSLTKAPQSALLNRTDSPRRETLRAELARAKPAGRRARLKPKLV
                     LASGSPRRLTLLAQAGIEPDGLRPASIDETPGKGEMPRSLVSRLARAKAESARDQIAN
                     DKDIADAHVLAADTIVAVGRRILVKPRFVEEALAALQLLSGRNHRVITGLCMITPDDR
                     VRLKIVDTRVRFKRLSKEEMDAYIASREWRGKAGGYAIQGLAGCFVQKLVGSYTNVVG
                     LPLTEVVGMLIAEGFPIHFNWLKMGETELS"
     gene            5677..5892
                     /gene="yacG"
                     /locus_tag="VFR00_04035"
     CDS             5677..5892
                     /gene="yacG"
                     /locus_tag="VFR00_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002717281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HEU0158454.1"
                     /translation="MSEAKPRADAPARCPICGRPSHADYRPFCSRRCADVDLSRWLRG
                     TYTIPGEPQSSEPSETLPAAKPRPEKS"
     gene            6167..6664
                     /gene="mraZ"
                     /locus_tag="VFR00_04040"
     CDS             6167..6664
                     /gene="mraZ"
                     /locus_tag="VFR00_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HEU0158455.1"
                     /translation="MDRFVSTFTNKIDAKGRVSIPAPFRAVLERDGYAGGIYCYPSLD
                     APALDAGGQSLAQKIDGLLAGLPDYSDERDELSVALYGDVHILSLDGDGRIVLPEALR
                     AHAGLATHVTFVGLGQKFQMWEPQRFEERRARARAKVQDHRRLFGAGSRLGTGDDGGA
                     GGARE"
     gene            6661..>7234
                     /gene="rsmH"
                     /locus_tag="VFR00_04045"
     CDS             6661..>7234
                     /gene="rsmH"
                     /locus_tag="VFR00_04045"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019996898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEU0158456.1"
                     /translation="MMTPGGTSMGEGARRGTSRHVPVLLPEVLAALEPRRGETYIDGT
                     FGAGGYAAAILTAAPGCRVLAIDRDRQAIAAGAELVQQHPGRLLLEEGRFGRLDHIAE
                     KSGLGQVDGVVLDLGVSSMQLDDPERGFSFQRDGPLDMRMSPQGPTAADFINTAGESE
                     LADVIFHLGGERSSRAIARRILARRTERPWA"
BASE COUNT         1202 a         2362 c         2363 g         1307 t
ORIGIN      
        1 ggcgctgatc attggcgcct ttgcgctggc gggctttttg tccggcatca ttatgccttc
       61 gcgcgatatg cttgttcgca aagccgcgcc gcccggcatg gttggacgcg cctttggcat
      121 cgtttccacg ggctttaaca tcggcgggat tatcgggcca ctcctgttcg gctggatcat
      181 ggacaacgcc tcgccgcgct gggtgttcgg cgcctgcgct gtctttatgg ccgtcacggc
      241 gctgttcggg atcgccagcg aggcacgcgc gcgccggcgc cttgccgctc aggtgagcgc
      301 gcgctagcct gtaaaggttg cgatgacccc gcaagcacag tggaccatac ggtgatggcc
      361 aagccgcacg aaagcaaggg atccgagcgc gagcgtcgac aagaaaggct ggcccagcaa
      421 ctgcgcagca accttgccaa acgcaaagcc caaagccatg ggcgtagtga ggtcgaggag
      481 ggcggcaagg tgcgtccagg cggcaacacg gcgcgggact cctgaggcgc gccgcatcaa
      541 cgccttgtcg ggtctgtcga atggtgcgtt gttgagctta cgcgcaaacg ggacgctgct
      601 atggatcgca tcaacatcgt cgggggtcgc cgcctgaagg gcacgattcc gatttcgggt
      661 gccaaaaatg cggctttgcc gctgatgatc gccagtctcc tgaccggcga ccggctgacg
      721 ctcaagaacg tgccgaatct tgccgacgtc aatcttttgg cgcgcattct gcgcaaccat
      781 ggcgtcgatc tcacgatcga tggaaaacgc gccgggcctt caccgtactt gggtgaaacc
      841 tttcacctga ccgcgcgcga tatcgtggat acgaccgcac cttacgagat ggtcaaccgc
      901 atgcgcgcca gcttttgggt gctggggcca ctgttggcgc gctgcggccg cgcgcgcgtg
      961 tcgatgccgg gcggttgtgc cattggcacc cgtcccgtcg acctgcacct gttcggcctc
     1021 aaggcgctgg gggccagtat tgatatcgac ggcggctatg tcgtggccaa agcccccaag
     1081 cgcctcaaag gcgccagggt ctcctttcct aaggtctccg tgggcgcaac ccacaccgtg
     1141 ctgatgagcg cggccctcgc cgatggggag acagtcatcg aaaatgccgc gcgcgagccc
     1201 gaggtcggag acgttgcgga gtgcctcgta aagatgggcg ccaggatcga gggcattgga
     1261 agcaagaccc tgcgcatcga ggggcgggag cgcctcgagg gtgccgtgca tacggtgctg
     1321 ccggaccgta tcgagacggg gacctacgcc ttcgccgtgg ccgcggccgg cggcgacgtc
     1381 gagctgaagg gggcccgcaa gggcttattg gaagcggcat tcgcgacgct tgaagccacg
     1441 ggcgtctcag ttgccgacac ggctgacggc gtgcgcattg cgcgcgacag cggcgagctg
     1501 gaaccgatca ggatcgagac ccagcccttt cctggctttc ccaccgatct gcaggcgcaa
     1561 ctcatggccc taatgacgcg ggccaagggc aacagcgtca ttcgcgagac gattttcgag
     1621 aaccgcttca tgcacgtgca ggagctggtt cgccttggcg cggatatcca gctgcagggg
     1681 gataccgcca tgatccgagg cgtgccgaaa ctccagggcg cacaggtgat ggcgaccgat
     1741 ttgcgggcct ccgtgtcgct ggtcattgcg gggctgatgg cggagggaga aaccatcatc
     1801 aaccgggtct atcacctcga ccgcggcttc gagcggctgg agcagaagct ctctggctgc
     1861 ggtgcgagta tcgcgcgctt ggtcagcggc tgatcgcacg cccccgccgg gcccgccatg
     1921 ctgccccggc gccgctggcg aggccctcgg cgagcacccc cttacaatcg tcggcgccgt
     1981 tcgctatcac tcgggcacgc ggcaggcttt gcccaggtcc tgctgcggcg atctatggca
     2041 cacaaaggac cgcttcacga catggccgag ctcaagctga tcgcgctcga tgccgacgac
     2101 ttggccgtgt tgtcggcaca cctccaggat gccgtgatcc aggtggcaga cattgcctat
     2161 ctcccaaagg agcggcgctt tgccgccatc ggcaatcggt ttgactggat ggccgcggcc
     2221 gatgccggcg ttggctatgc ccgccgccgg gcggcggtgc gtttcgagcg ggtgctcggc
     2281 gcgcaagtgc aggcgatcga tcttaagaat aaagcttcgg tcctctcctt gctcgccgtc
     2341 accttcgagc cgaccacggc gccggaaggc tacgtgacct tgcattttgc cgatggcggg
     2401 gccatccggc tgcacgtcga gtgtgtcgaa gccgagctta aggatttggg tccgatctgg
     2461 cgcgtcgact tcaaacccca gcatcccgac ggcagcccgg tgtcgccctc gcccggcaaa
     2521 gagcgctcca cctgaggttg accctcaccc aagcccatgc ccattcagct cgccagcact
     2581 gatgtcaatt tcgaagcggc gttcgccagc tttttggcga gcaagcgcga agtctcgccc
     2641 gacgtcgatg agcgcgtgcg tgccatcatt gccgacgtgc gtacccgcgg cgacgcggcg
     2701 ctgatcgagt atacgcgccg cttcgatcag ctcgatctcg cgagcgtggg tattcgcctc
     2761 gacgaagccg cgatccgcgc ggcccggcga gactgcgacc cagccacgct cgatgccttg
     2821 cttttggcga aggcccggat cgaggcgtac cacctgcgtc aagttcccaa ggacgaaagt
     2881 tttcgtgatc ctgtcggtgc cgaactcggc tggcgctatt ccccgatcga gtccgttggc
     2941 ctctatgttc cggggggcct tgccagctac ccgagttcgc tgctgatgaa tgccgtgccg
     3001 gccaaggtcg ctggcgtgcc gcgtattgcc attgcggtgc ccgcgcccag gggtgaactc
     3061 aatcccttcg tgctggcggc cgccgatatc gccggcgtca cggagatcta ccgcatgggt
     3121 ggtgctcagg cggtggcagc cttcgcctat ggcacccaaa gcgtgccgca agtggccaag
     3181 attgtgggtc cgggcaatgc ctacgtcgcc gccgccaagc gccaggtgtt cggcgtggtc
     3241 gggatcgacg ccatcgccgg cccctcggag gtgctcatca ttgccgatgc tgccagcgat
     3301 cccgtgtggt tggccgccga cttgctcgcg caggccgagc acgacactgc cgcgcaagcc
     3361 attctgatga cggatgatgc cacgcttgcc gcccgcgtcg aagccgaagt tgagcgtcaa
     3421 ctcggtctct tgccgcgccg cgacatcgcg gcggcaagct ggcgcgagtt cggggcgctg
     3481 atcgtgctgg agtccctgga gcaagccccc gccctggccg accgcattgc cgccgagcat
     3541 gtgcagatcc tgacgcggga tcccgatcgt cttgccggcc aaattcgcaa tgccggtgcg
     3601 gtctttctcg gtgcccacac gccggaagtc attggcgatt acgtggccgg ctccaatcac
     3661 gtgttgccga cggcgcgtag cgcgcgcttt gcctccggcc tcggtgtgca agacttcctg
     3721 aagcgcacct caactctgcg cctcgacgcg gcggcgctga gccggcttgc cgcccccgcc
     3781 atggttttgg cccgtgccga aggtctcacc gcccatgagc gctcggtggc ggtccgcctc
     3841 ggcttgagtg ccgaacgctc ttgaggtctc aatgtttgag ccgccggcaa gcgatgccaa
     3901 tcccaaggcc cggctcaccg ccattacgct cgacagcacc tcgatcaagc gcgcgacgtc
     3961 gggtatcgag cacgagcgcg aggttgccat ttacgacatc ctggagagca actttttcag
     4021 cctcgagggc cgcgacgaag gcccgtatac cctcaatctc tccatcgccg aggaccgcct
     4081 ggtgctggcg gtcgggcaag cggcgggcga cagcgtcttc acggtggtat tgtctctgac
     4141 accgctcagg cgcgtcatga aagactattt cattgtgtgc gaaagctact accgggccat
     4201 tcgcacggca ccgccgtcgc gcatccagtc catcgacatg gggcggcgcg ctttacacga
     4261 tgaaggcagt cgcgtgttgc tcgagcggct caagggtaag atttccgtgg acaacgatac
     4321 ggcgcggcgc ttgttcacac tgatctgtgc gctgcactgg aaaggatgag gtgtggcggg
     4381 cgcggggggc caagagctgc cgggcgcggt cttgttcgcc tgcaccatga actcggtgcg
     4441 ctcccccatg gccgcggcga tcctaagaca ccttgccggc cggcgtgcct acgtggaatc
     4501 ggcgggcgtg cgcgccggtg gcttggacgc cttcgccgtg gccgcgatgg aggaaatcgg
     4561 catcgacatt tcccaacacg caccgcgcac cctgcgcgag ctgaacgaca cgtccttcga
     4621 cctgatcatt tcgctctccc ccgaggccca ccaccaagct ctcgagctga cccgcaccat
     4681 ggcggtggac gttgagtact ggccgacctt cgacgcgacg ctgctgatcg gcagcgcgag
     4741 ccgcgagcaa attctgcaag gctatcggga ggtgcgcgat cagctgtttc agcgtattaa
     4801 gcgccgcttc ggcttcgagg gcggcccgag cgtttagccc gttggtgtcc ccgcactcgg
     4861 cttgattttc cgcgccgacc gtgcccaatg tcggccgctc tcatgcgctt ggagctacgc
     4921 ctatgagcct gacaaaggcg ccacaatcgg cgctcttgaa ccgtacggat agtccgaggc
     4981 gcgaaacgct gcgcgccgag ctggcgcgcg ccaaacccgc tggacggcgc gcccgcctca
     5041 aacccaagct ggtgctcgcc agcggatcgc cgcgccggct caccttgctg gcgcaggcgg
     5101 ggatcgaacc cgacgggctc aggccggcct cgatcgacga gacgccgggc aagggcgaga
     5161 tgccgcgttc gctggtatcg cgcctggcgc gcgccaaggc ggagagcgcc cgcgaccaga
     5221 tcgccaatga caaggatatt gccgatgccc atgtgctggc cgccgatacg atcgtcgccg
     5281 ttggtcggcg cattctggtc aaaccgcgct tcgtcgagga agcgttggca gcgcttcagc
     5341 tcctatcggg ccgcaaccat cgcgtgatta ccgggttgtg catgatcacg ccggacgatc
     5401 gcgtgcggct caagatcgtc gacacacgcg tacgcttcaa gcggttgtcc aaggaggaaa
     5461 tggacgccta catcgcctcg cgcgaatggc ggggcaaggc gggcggttat gcgatccagg
     5521 gtttggcggg atgctttgtg caaaagctgg tcggctcgta taccaacgtg gtgggactac
     5581 ccttgaccga agtggtgggt atgctgatcg cggaaggctt tccgatccac ttcaattggc
     5641 tcaagatggg cgagaccgag ctctcatagg cctcccatga gcgaggcaaa accccgcgct
     5701 gacgcgcccg cacgctgccc gatctgcggg cgaccgagcc acgccgatta tcgtccgttc
     5761 tgctcgcggc gttgcgccga tgttgacctt tcgcgctggt tgcgcggcac ctatacgatc
     5821 cccggcgaac cgcagtcctc cgagccgagc gagacgttgc cggcggcaaa gccgcgcccc
     5881 gaaaaaagct aaggcttttt gcccagccca tcagaggcgt aggcgccgcg ccaagggccg
     5941 cgcggcgacg acccgcgtgc acttggcgcg caacgttgcc ccgcccgcgt gacaggagcg
     6001 ccgaccgctg tggcgttccg accaccacgg cgcagaattt cccatagttg tgcacccaag
     6061 gcccgttgac tcccatcaaa tcccatgtaa tcccagatca gcccgtcgga tgaccgcggg
     6121 aattgtgttg gtggcggagc gtgccagcga ggctcgagcg caaaggatgg atcgctttgt
     6181 ctccaccttc accaacaaga tcgatgcgaa agggcgcgtg tcgatcccgg ccccgttccg
     6241 ggccgtcctc gaacgcgacg gctacgccgg cggcatctac tgctacccct cgctcgatgc
     6301 tccagcgctc gatgcaggcg gccaaagcct cgcacaaaaa atcgatgggc ttctcgctgg
     6361 cctgccggat tattcggacg agcgggacga actgtctgtc gcgctctatg gcgacgtcca
     6421 catcctcagc ctcgatggcg acggacgcat cgtccttcct gaagcgcttc gcgcgcacgc
     6481 cggacttgcg acccatgtca cttttgtcgg cctgggtcag aaatttcaaa tgtgggaacc
     6541 gcagcgcttc gaagagcggc gcgcgcgggc ccgcgccaaa gttcaagacc accgccgcct
     6601 tttcggcgcg gggagccgcc tcggtacggg ggacgatggc ggcgcgggag gagcacggga
     6661 atgatgacgc cgggcggcac ctcgatgggg gagggtgctc gcagagggac atcgcgccac
     6721 gttcccgtgc tgcttcccga agttctggcc gcgctcgagc cgcggcgcgg cgagacctac
     6781 attgatggca ccttcggcgc tggcggctat gccgccgcga tcctgaccgc agcgccgggt
     6841 tgccgcgtcc tggccatcga tcgggatcgg caggcaatcg cggccggcgc cgagctggtg
     6901 caacagcacc cggggcggct gctcctcgag gagggtcggt tcggtcgtct cgatcacatt
     6961 gcggaaaaaa gcggcctggg ccaggtcgat ggcgtcgttc tcgatctcgg tgtttcgtcg
     7021 atgcagctcg acgacccgga gcgtggcttt tcttttcagc gcgacggacc gctcgacatg
     7081 cgcatgtccc cgcaggggcc gaccgccgcc gatttcatca ataccgcggg tgaaagcgag
     7141 ctcgccgatg tgatctttca tctcggcggc gagcggagct cgcgcgccat cgcgcgtcgg
     7201 atcctggcgc gccggaccga gcgaccctgg gcgc
//
LOCUS       DASTMT010000181         2254 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_428169, whole genome shotgun sequence.
ACCESSION   DASTMT010000181 DASTMT010000000
VERSION     DASTMT010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2254
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_428169"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            368..2089
                     /locus_tag="VFR00_04050"
     CDS             368..2089
                     /locus_tag="VFR00_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018643996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HEU0158457.1"
                     /translation="MNDVTKTSGGGRQDMGRAIHPGSGRRKSAPFPKGRVLSQAEVEK
                     VRALLGDAPRERALLIEFLHLIQDHEGALPAGLLHALAEELAIPMAEVYEVATFYAHF
                     DVVADGEAKPEKITIRVCDSLSCMLAGADALVAALTAKQLPGVRVVRAPCIGSCATAP
                     AAEVGHRHVDHATVATLAALAGTGDVHPAIPAYQDFAAYSQAGGYAVLRSCLAAQRTP
                     EAVLATLSDGGLRGLGGAGFPTGRKWSLVRAEPAPRLMAVNGDEGEPGTFKDRYFLER
                     EPHKFLEGMLIAAWAVEAERVFIYLRDEYPAVLKLLESELSKLEAAGLSKHTQVHVRR
                     GAGAYICGEESAMIESIEGKRGYPRHRPPYVAQVGIFGRPTLVNNIETLYWVPSILEK
                     GAPWFAAQGKNGAKGLRSYSVSGRVRKPGVVVTAAGSTARDLIELCGGMADGHNFKGY
                     LPGGASGGILPAYMADLPLEFGQLEKHGAFVGSHAVVILSDQDDMKAVALNLMRFFED
                     ESCGQCTPCRNGTEKAVALLASGNWDADLHADLAQVMADASICGLGQAAPNPVRSVFR
                     YFPEDLR"
BASE COUNT          385 a          743 c          735 g          391 t
ORIGIN      
        1 cgtgcgaacc gaacaacgag ccgacccact ccgcccactc atcggtgagc ggcgcaagca
       61 ccacgctgcc accaagcgag gccaacaggt ccatcgcact tcccttctcg atcgccgatc
      121 gccttcatgg cctaacttgg aggtgggctg tcaaggataa tgcgacatcg tcgcggcgag
      181 ccaagcggtc gcagggcttg gctgccgtgc gcgccgctga tgatcctccg acatctgatg
      241 attgtcgggg gcccgcaagc ttcgcctcgg ctgccgccgg gccggcaacc agcgcccctc
      301 gacaccatcg cgacccctgg tatatggtat gccaagccgg taggcacggc gatcccattt
      361 ggtgagtatg aacgacgtaa cgaagacctc gggcggcggc cgacaggaca tgggccgagc
      421 gatccatcct ggctccggcc gccgcaaaag cgcacctttt cccaagggcc gcgtactcag
      481 ccaagctgag gtcgagaagg tgcgcgccct gttgggcgat gcgccccgcg agcgcgcgct
      541 tttgatcgaa tttctgcacc tgatccagga ccacgaagga gcgctgccgg cgggcctgct
      601 gcacgcgctc gccgaggagc tcgccattcc catggcggag gtctacgagg ttgcgacctt
      661 ttatgcccat ttcgatgtgg ttgccgacgg cgaggccaaa ccagagaaga tcaccatccg
      721 ggtctgtgac agcctcagct gcatgctggc gggtgcggac gcgctcgtgg cggcgttaac
      781 ggcaaaacag ctgccgggtg tgcgggtggt gcgcgcgccc tgcatcggct cctgcgccac
      841 cgcgcccgcc gccgaagtcg gtcatcgcca cgtcgatcat gcgaccgtgg ccacgctcgc
      901 ggcgctggcc ggcaccggcg acgtccaccc cgcgatcccc gcctaccaag attttgctgc
      961 ttacagccaa gcggggggct atgccgtcct gcgctcctgc ctcgccgctc agcgcacgcc
     1021 cgaggcggtg ctcgcgaccc tgtcggacgg cggtttgcgc ggcctcggcg gcgccggctt
     1081 cccgacgggc cgcaagtgga gcctggtgcg cgcggagccc gccccgcgcc tgatggcggt
     1141 caacggcgac gagggtgagc ccggcacctt caaggaccgt tattttctcg agcgcgagcc
     1201 gcacaagttc ctcgagggca tgctgattgc cgcctgggcg gtggaggccg aacgggtctt
     1261 catctacctg cgcgatgaat accccgctgt gctcaaactg cttgagagcg agctaagcaa
     1321 gctcgaggca gcgggcctca gcaaacacac ccaggtgcac gtgcgccgcg gcgcgggcgc
     1381 ttacatctgc ggcgaagaaa gcgcgatgat cgaatcgata gagggcaagc gcggctatcc
     1441 gcgccatcgc ccgccctatg tggctcaggt cggcattttc ggtcggccta cgctcgtcaa
     1501 caacatcgag acgctctact gggtgccgag catcctggaa aagggggcac cgtggtttgc
     1561 cgcgcaaggc aagaacggcg ccaaggggct gcgctcctat tccgtatccg gccgggtcag
     1621 aaagccaggc gtggtcgtga cggcggcggg gtccacggca cgcgacctca tcgagctgtg
     1681 cggcggtatg gccgatggcc acaatttcaa aggctatttg cctggcggcg cctccggcgg
     1741 cattttgccc gcctatatgg ccgacctccc gctcgaattc gggcagctcg agaagcatgg
     1801 tgcctttgtc ggcagccacg cggttgtgat tttgtccgac caggacgata tgaaagctgt
     1861 ggctttgaac ctgatgcgct tcttcgagga cgagagttgc gggcaatgca caccgtgccg
     1921 taacggcacg gaaaaggccg tcgccttgct ggcgagtggc aactgggatg ccgacctcca
     1981 tgccgacctt gctcaggtca tggcggatgc ctcgatctgc ggcttgggtc aggctgcgcc
     2041 caatccggtg cgctccgtgt ttcgctattt tcccgaggac ctgcgctgat gaggctgccc
     2101 caagccgtgt ccacgctgca gatcgacaat gaccgcgtgc gcgtcactga atggcgcttc
     2161 gcgccaggag cggaaaccgg cgagcacgtg catcagctcg actacgtcgt ggtaccgctg
     2221 gcgacgggcc gactgaagat cgtttccccc aagg
//
LOCUS       DASTMT010000182         3748 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_2392907, whole genome shotgun sequence.
ACCESSION   DASTMT010000182 DASTMT010000000
VERSION     DASTMT010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3748)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3748)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3748
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2392907"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            114..1088
                     /locus_tag="VFR00_04055"
     CDS             114..1088
                     /locus_tag="VFR00_04055"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018455964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HEU0158458.1"
                     /translation="MPDNPAAVLEVNGLKKYFPVRRGLLRRTVGHVHAVDDVSFTIRA
                     AETVGLVGESGCGKTTVGRTILRLIEPTAGSIRLEGQELSSLSKAELRPLRQRMQIIF
                     QDPFSSLNPRMRAGDIVAEPLKLHGDDGKRARRERVAELFERVGLRRAQMDNYPHQFS
                     GGQRQRIGVARALALNPRLIVGDEPVSALDVSIQAQVINLLRDLQRDLQLSYLFISHN
                     LAVVEHISHRIAVMYLGRIVEYTDKTTLFARPLHPYTEALLAAVPIPNPAIKRTRRVV
                     QGDVPSPLRPPPGCHFHTRCPYAEARCRSELPLLREVQPGHHVACHLR"
     gene            complement(1100..2320)
                     /locus_tag="VFR00_04060"
     CDS             complement(1100..2320)
                     /locus_tag="VFR00_04060"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018259974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU0158459.1"
                     /translation="MRILFVHQNFPGQFPHLAPALMARGHEVTALTVASNKRPSPVPV
                     LHYTYTKPNFERSISRHVANFADRAERGVAVAQAAEKLGAKGYVPDVIFGHFGWGETL
                     YLKNVWPAAKLALYAEFFYHPRGLDADFDPEIQQPNLASNIVTTTLQAPLLLAMHTAD
                     AAIAPTRWQAETFPAHFQERITVIHDGIDTERLRPNPAAWVTVGSRIFRPGDEVVTFV
                     SRNLEPYRGYHIFMRALPEVLRARPNAHVVIVGGQEAGYGPLHASRKTWKQIFIDEVK
                     DQLDPTRVHFVGTIPHTTFVNLMSVTQVHVYLTYPFVLSWSMLEAMSAGALVVGSATP
                     PVKEIIRHGSNGLLVDFFDLPRWSATLIETLSEPAKFLPLREAARQTVLEGYDLKGNC
                     LPRMIEFVENLARA"
     gene            complement(2365..3480)
                     /locus_tag="VFR00_04065"
     CDS             complement(2365..3480)
                     /locus_tag="VFR00_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009493425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HEU0158460.1"
                     /translation="MPTYRNLLPGDPAPWFRQRSSVNPSYAFDSAAGRYLVLCFFLSA
                     ANPRARAALDAAALQRSLFNDDKAAFFGVSIDPADEQQKRVATKLPGYRYFWDTDSKV
                     STLYGAVPVDGPQTGAGARQLWVVLDPTLRILKVVPFREDGSSTAEVLGFLEQLPPPA
                     RFAGFEIQAPVLILPNVFEQEFCDRLIALYQADGGQESGFMREKDGKTVVAHDPGHKR
                     RKDYEIADMEVVKHAQNRVIRRVAPEILKVHQFQVTRMERYIVSCYAEEDGGHFAPHR
                     DNTTKGTAHRRFAVSINLNADFDGGEVSFPEYSGRGFKPPPGGAVVFSCSLLHTVSKV
                     RRGRRYAFLPFLYDETSAKLREANNSFLGEGVGAYRR"
     gene            complement(3562..>3748)
                     /locus_tag="VFR00_04070"
     CDS             complement(3562..>3748)
                     /locus_tag="VFR00_04070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="M23 family peptidase"
                     /protein_id="HEU0158461.1"
                     /translation="LDQRARALERNEAQSLFFAGKKRPAGGWPAGVYRASYRVLVAGA
                     PVLERSFSLALGPVSAP"
BASE COUNT          688 a         1199 c         1223 g          638 t
ORIGIN      
        1 gcgctgcgcc tttgccagcg acgtgtgccg ccagcagtac ccgcgctacg aagaaaaaca
       61 gggcggccat tgggccgcct gctggcactc cgacaagctc gctggagccg ccaatgcctg
      121 acaatccggc cgccgtgttg gaggtcaacg gactgaagaa atactttccc gtccggcgcg
      181 ggctgctgcg gcgcactgtg ggccatgtgc acgccgtcga cgacgtcagt ttcactattc
      241 gtgccgccga aaccgtgggc ctggtcggcg aatcgggatg cggcaaaacc acggtcgggc
      301 gcaccatcct gcgcctgatc gagccgaccg cgggcagcat tcggctcgag ggccaggagc
      361 tctcaagcct cagcaaggcc gagttgcggc ccctgcgcca gcgcatgcag atcatcttcc
      421 aagacccctt ctcgtcgctc aatccgcgca tgcgggccgg cgatatcgtc gccgaaccct
      481 tgaagctgca cggtgacgac ggcaagcggg cgcggcggga gcgcgtggcg gagctgttcg
      541 agcgcgtcgg gctgcgccgc gcccaaatgg acaactatcc gcaccagttc tccggcgggc
      601 agcgccagcg catcggcgtg gcgcgcgcac tcgccctcaa tccacgcctg atcgtcggtg
      661 acgaacccgt ctccgccctc gacgtgtcga tccaggccca ggtcatcaac ctgttgcggg
      721 acttgcagcg cgatttgcag ctgtcctacc tttttatctc gcacaacctt gcggtggtgg
      781 agcatatcag ccatcgcatc gccgtgatgt atctcggccg gatcgtcgag tacaccgaca
      841 agaccaccct gttcgcgcgg cccctgcacc cctacacgga ggcactgctt gccgccgtac
      901 cgatacccaa tccggccatc aagcgcaccc gccgcgtggt gcaaggcgac gtgccaagtc
      961 cgttgcggcc gccaccgggt tgtcattttc atacgcgctg cccctacgcg gaggcgcgtt
     1021 gccggtcgga gctgccgttg ctgcgcgagg tgcagccggg ccaccacgtg gcctgccatt
     1081 tgcgctagat cgggtggcgc taggcgcgcg ccaggttctc gacgaattcg atcatgcgcg
     1141 gcagacaatt gcccttaagg tcgtagccct ccagcacggt ctgtcgcgcg gcctcacgca
     1201 gcggcagaaa cttggcaggc tcggagagcg tttcgatcag cgtcgccgac cagcgcggca
     1261 ggtcgaagaa gtcgaccaat agcccgttcg atccatgccg gatgatctct ttgaccggcg
     1321 gcgtcgccga gccgacgacg agggcgcccg cgctcatggc ctccaacatc gaccaggaca
     1381 gcacaaacgg gtaggtgaga tagacgtgca cttgcgtcac gctcatcagg ttgacgaagg
     1441 tggtatgtgg aatcgtgccg acaaaatgca cccgcgttgg gtcgagctga tccttgacct
     1501 cgtcgataaa gatctgtttc caggttttgc gcgaggcgtg cagtggccca taaccggctt
     1561 cctggccgcc gacgatgacg acgtgagcgt tgggccgcgc cctcaacacc tcgggcaggg
     1621 cgcgcatgaa aatgtggtag ccgcgatagg gctcgaggtt gcggctgacg aaggtcacga
     1681 cctcatcgcc cggtcggaaa atccgactgc cgaccgtgac ccaggcggcc ggattgggcc
     1741 gcagacgctc cgtatcgatg ccgtcgtgaa tgaccgtgat gcgctcttgg aagtgggcag
     1801 gaaaggtctc cgcttgccag cgcgtcggcg cgatcgccgc gtcggcggta tgcatcgcca
     1861 gcagcagggg tgcctgcagg gtggtggtga cgatgttgct ggccaagttg ggttgctgga
     1921 tttctgggtc gaaatcggca tcgagcccgc gcggatggta aaaaaattcg gcatagagcg
     1981 cgagcttggc ggcgggccac acgtttttca ggtagagcgt ttcaccccag ccgaaatggc
     2041 cgaagatgac gtcgggcaca taacccttgg cgcccagctt ttcggcggct tgcgcaacgg
     2101 cgaccccgcg ctcggcacgg tcggcaaagt tggcgacgtg ccgggagatc gagcgctcga
     2161 aatttggctt tgtgtaagtg tagtggagca ccggcaccgg cgagggccgc ttgttgcttg
     2221 ccaccgtcag ggcggtcacc tcgtgcccgc gggccatgag cgccggcgcc aggtgcggaa
     2281 attgccctgg gaaattctgg tgcacgaaca gtatccgcac gcggggaccc ccacctcaat
     2341 ctcggcccga gacggcagcg acgattagcg ccgataggcg ccaacgcctt caccaagaaa
     2401 ggaattgttg gcctcgcgca gctttgccga cgtctcgtcg taaaggaatg gcaggaaggc
     2461 ataacggcga ccgcgccgca ccttggacac ggtgtgcagc agcgaacagg agaacacgac
     2521 cgccccgccg ggaggcggct tgaagccgcg ccccgaatac tccggaaagc tcacctcgcc
     2581 accatcaaag tcggcgttga ggttgatcga cacggcaaag cggcggtgcg cggttccctt
     2641 ggtggtgttg tcgcgatgcg gcgcgaaatg tccaccatcc tcctccgcat aacagctgac
     2701 gatgtagcgc tccatgcgcg tgacttggaa ttggtggacc ttaaggattt ctggggcaac
     2761 ccggcggatg actcggttct gggcgtgctt gaccacctcc atgtcggcga tttcgtagtc
     2821 cttgcgccgc ttgtggccgg ggtcgtgcgc caccaccgtc ttgccgtcct tctcgcgcat
     2881 gaagcccgat tcttgcccgc cgtccgcctg gtagagggca atgagccggt cgcagaattc
     2941 ctgctcaaac acgttgggta ggatgagcac gggcgcctgg atttcaaagc ccgcaaagcg
     3001 cgccggcggc ggcagttgct cgagaaagcc aagcacctcg gccgtgctgc tgccatcctc
     3061 gcggaagggc acgaccttga ggatgcgcaa ggtcgggtcg agaacgaccc agagctggcg
     3121 agcgccggcg ccggtctgcg gcccatcgac cggcacggcg ccgtacagcg tgctgacctt
     3181 gctatcggta tcccaaaaat aacggtagcc cggcagcttg gttgccacgc gcttttgctg
     3241 ttcgtcggcc ggatcgatgc tgaccccgaa aaaggccgcc ttgtcatcgt taaacaggga
     3301 gcgctggagg gcggcggcgt cgagcgcggc gcgcgcgcgc gggttggcgg cgctcagaaa
     3361 aaagcacagc acgagatagc gtccggccgc cgaatcgaag gcatagcttg ggttcaccga
     3421 cgagcgctga cgaaaccaag gtgccggatc gccgggcagc agattacgat aggtcggcat
     3481 aacaaactcc ccaagggcgg cacactaaca aagcccccct tgcatttgaa ggcacgccgg
     3541 cgcggcgccc gctaacatgg ttcacggggc ggaaaccggc ccgagcgcca gcgaaaagct
     3601 gcgctcaagc acgggcgcgc ctgccaccag cacccggtag ctggcgcggt agacgcccgc
     3661 cggccaaccg cccgcagggc gcttcttgcc ggcaaagaag aggctttgcg cttcgttgcg
     3721 ctcaagggcg cgcgcacgct gatcgagc
//
LOCUS       DASTMT010000183        10006 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_4644792, whole genome shotgun sequence.
ACCESSION   DASTMT010000183 DASTMT010000000
VERSION     DASTMT010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 10006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10006
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4644792"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..681
                     /locus_tag="VFR00_04075"
     CDS             <1..681
                     /locus_tag="VFR00_04075"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HEU0158462.1"
                     /translation="HETGFGLLKAAAALNVRPIPFGKSHELKERDPVLVASFGGPDMV
                     APAFVVAKREFAGSWEYLLEEAIFTAPPHPAWSGAALINREGKLVGVGSLIVGDAAGG
                     TPGNMFVPIDRLPPIMADLLSEGRVQGPARPWLGLTTEENGGKLLVGRVAPGGPAEKA
                     GVQKGDVVVGVDGRPATGLADFYRKVWSLGAAGTVIPLDVQQNNEKRRFELRSIDRMD
                     HLRFKSTF"
     gene            788..1678
                     /locus_tag="VFR00_04080"
     CDS             788..1678
                     /locus_tag="VFR00_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006610246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEU0158463.1"
                     /translation="MSWLWAVFTVLAAGGQVLRNAQQKELTGTLGTVGATHVRFLFGL
                     PFGLLFLALVLLATGQGLPPLNAAMLAWSAAAALAQISATALLLAAMRDRSFVVTTAY
                     AKTEPVQVAIFGLAFLGDRLSPGLALAIGIATAGVLIMSWPRRSAEEAFSWHPALLGI
                     ASGAMFAIAAIGFRGGIRALNAPNFVLAATTTLAFGLIIQTALLSAYLALFDRAKLIA
                     ICREWRPSMLAGFTGAFASQMWFLAFALESAAKVRTLALVEVLFAQILSRNLFKQRLA
                     SREALGILLIVAGVVLLLNL"
     gene            1717..2073
                     /locus_tag="VFR00_04085"
     CDS             1717..2073
                     /locus_tag="VFR00_04085"
                     /inference="COORDINATES: protein motif:HMM:NF024636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2TM domain-containing protein"
                     /protein_id="HEU0158464.1"
                     /translation="MAPREMGTQAPPRDAAREAARLENARRRLAALKGFYVHLIVFVL
                     VIAGLLIINSLTGGPWWVALVFFAWGLGVLAHGLALVARRSRAIAAWEQRKLAAYLAE
                     DSGGPPGSRHDPNRPI"
     gene            complement(2066..2671)
                     /gene="gstA"
                     /locus_tag="VFR00_04090"
     CDS             complement(2066..2671)
                     /gene="gstA"
                     /locus_tag="VFR00_04090"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006022908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HEU0158465.1"
                     /translation="MKLFYAPGACSLSPHIVLNEAGLKFDKVKVDTKSKAMEGGDYRS
                     INPLGYVPALELDDGTILTEGPAIVQWIADKVPDKQLAPASGSLERTKLQSWLNFIST
                     ELHKGFSPLFNPNMPDAAKQVFRERLATRFQHLDKHLADNDYLMGKAFSVADAYLFTV
                     SNWAAHTGVDLSGFANVLAYRKRVGARAAVQAAMQAEGLIK"
     gene            2808..3242
                     /locus_tag="VFR00_04095"
     CDS             2808..3242
                     /locus_tag="VFR00_04095"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HEU0158466.1"
                     /translation="MQAKPTTGVLKAWISLLRTERELVDKVEECLKRAGLPPLACYHV
                     LDAVERAPKGMLRQAQVQAGTQLAQYTLCRLLDRLERDGLMERHQCQRDGRNNVLLLT
                     SKGRAMRRSIWPVYAKAIEEHFGSRLSSAEAEQLASLLGKLG"
     gene            3358..4206
                     /gene="fabI"
                     /locus_tag="VFR00_04100"
     CDS             3358..4206
                     /gene="fabI"
                     /locus_tag="VFR00_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004318 - enoyl-[acyl-carrier-protein]
                     reductase (NADH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HEU0158467.1"
                     /translation="MAGPAAQADSPAGTLMQGKRGLIMGVANNRSIAWGIAKATAAQG
                     AKLAFTYQGEALKKRVEPLAAEAGSEIVLPCDVADAGSMDAVFAELGRRWGELDFLVH
                     AIAFSDKAELDGRYIDTSAKNFNQTMLISCYSFTALAQRAEKLMRQGGALLTLTYYGA
                     EKVMPHYNVMGVAKAALEASVRYLAADLGKGGIRVNAISAGPIKTLAASGIADFRYIL
                     KWNEYNSALRRTVTIEEVGAAGLYLLSDLSRGVTGEVHHVDAGYHVQGMKNEEAPDIS
                     VVRDTT"
     gene            complement(4232..4534)
                     /locus_tag="VFR00_04105"
     CDS             complement(4232..4534)
                     /locus_tag="VFR00_04105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0158468.1"
                     /translation="MRFAIITAAAAALASLAAANSASAQVGFGVYVGPSGYYDDYDYA
                     PPPPRARVYGYTSTYDYDARPDVVVRVPGRRGGCGEYFYWNGVRCVDARTSPPDLR"
     gene            4705..5679
                     /locus_tag="VFR00_04110"
     CDS             4705..5679
                     /locus_tag="VFR00_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013948604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0158469.1"
                     /translation="MRAVTALIITLIALGAPATAAAPTNKATAAAKKGEFSSSESILR
                     WINGYRLKPEPAKAPAAARAMSELNLFRDLETAGVYVGFLAGVLETNPKKAEELIAKM
                     FPMPPEDQVVIVRAIAYSNLPEWKALLLKFAERMPARQGLIDRFVHDKMPTLKQIPLD
                     SGSAPLDMLWGKYFATGSYEPVMRIVSILEWSKDANNVDRLTVGSMAKLTLATNASRD
                     KELMDMLKVSMKHETKENRAVLQEVIDAAETFEFGRVRKEAMGAIDQLKIKGPASARN
                     VQWWGTAGQTALALGCIVAGALGHVEIGIPCVVGGAVSGAALKYMTPQ"
     gene            complement(5700..7508)
                     /locus_tag="VFR00_04115"
     CDS             complement(5700..7508)
                     /locus_tag="VFR00_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation:proton antiporter-2 (CPA2)
                     family protein"
                     /protein_id="HEU0158470.1"
                     /translation="MSDVTLTQVALFLAAAVVAAPLAKLLRIGNVLGYLLVGVLIGPF
                     GLGFVVTVYEVTTVLHFAEFGVVLLLFLIGLELRPKRLWAMRAAIFGLGGAQVALTGL
                     ALAFVAMAFGLAWQAAVFAGLALSLSSTAFALQILEEKGELALRHGRLAFAVLLFQDL
                     AAIPLIAVPALFAVSVKDATQQMELLSAAKAIGVIVLVVVIGRYLLDGIFSLVARTRV
                     KEAMTASALLTVVGVTLVMEQAGLSASLGAFIAGVLLADSQYRHEIEADIAPFEGLLL
                     GLFFTAVGMSLNLGLIGQSPWLVAWVVAGLLAIKGLILYGLGRRAGLETGPARRFALA
                     ISQGGEFAFVLFASGMASGVLERSVADLLSVAVTLSMAATPLLLLLEETLLPKPVVAR
                     AFDAIPANDGHVVIAGFGRVGQIVARILTAKKFAITALDSSPEQVAVVRKFGAKIYYG
                     DASRHEILEAAQTDKARAFVLAVDNVEASVRIAQMVRQHYPHVPIYARARDRRHTHRL
                     MDLGVTAIRRETFLSSIELTRDLLRGFGLSQREVSFVVETFKAHDRRRLYEDYRHYTD
                     VERLQARARTSAQELEDLFAQDAAEQARAEQEQKAS"
     gene            complement(7706..8350)
                     /locus_tag="VFR00_04120"
     CDS             complement(7706..8350)
                     /locus_tag="VFR00_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018041991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEU0158471.1"
                     /translation="MFDVCRRDLIALLGAAGLIGLLAAAGAMADPSAQARLGAPAPDF
                     TLTDSNGRSVSLADFKGKTIVLEWTNHDCPYVRKHYGSQNMQALQKKWTAQGVAWLTL
                     ISSRPGAQGFVHGLEANKLTADRGAAPTAVLLDPNGSVGRSYGAQVTPHMYVITGDGA
                     LAYMGGIDDKPTTRLEDLKTAKNFVDAALGELALGSPVSVKTSRPYGCTIKYSS"
     gene            complement(9039..9215)
                     /locus_tag="VFR00_04125"
     CDS             complement(9039..9215)
                     /locus_tag="VFR00_04125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0158472.1"
                     /translation="MEALVHRLRRCADVQAVALYLLQRALEVCQCDFGNVQLINWETG
                     QLQIKAQQGFATSS"
     gene            complement(9536..>10006)
                     /locus_tag="VFR00_04130"
     CDS             complement(9536..>10006)
                     /locus_tag="VFR00_04130"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HEU0158473.1"
                     /translation="WWPGMAYQLALLQGDPAAVSDPGLVAAAERGRHIKAVELIGAAL
                     TRAELHHRMARFFASYDLLLTPTLPLAAFEVAHLLPPAGAWGEDWTGWAPFSYPFNLT
                     QQPAASLPCGFTREGLPVGLQVVGAIGRDDLVLRASRAFETCHPFVMPETPRSI"
BASE COUNT         1799 a         3268 c         3250 g         1689 t
ORIGIN      
        1 cacgaaaccg gctttggtct gctcaaggca gcggcggcgt tgaacgtgcg cccgatcccg
       61 ttcggcaaat cgcacgagct caaggagcgc gatcccgtcc tggtggcaag cttcggcggg
      121 cctgacatgg tggcacccgc cttcgtggtg gccaaacgcg agttcgccgg cagctgggaa
      181 tacctgctcg aggaggcgat ttttacggca ccaccgcatc ccgcctggag cggcgccgcg
      241 ctcatcaatc gcgagggcaa gctcgtgggc gtcggctcgc tgattgtcgg ggacgccgcc
      301 ggcggcacgc caggcaacat gtttgtgccg atcgaccggc tgccccccat tatggccgat
      361 cttctttccg agggccgcgt gcaaggcccg gcgcgacctt ggctgggtct gacgacggag
      421 gaaaacggcg gcaagctgct ggtcgggcgc gtggcaccgg ggggcccggc cgagaaggcc
      481 ggcgtgcaaa agggcgacgt cgtcgtgggc gtcgatgggc ggcctgccac gggccttgcg
      541 gacttctacc gcaaggtctg gtcgctcggt gcggccggca cggtgatccc cctcgacgtc
      601 cagcaaaaca acgaaaagcg tcgcttcgag ctcaggtcga tcgaccgtat ggatcacttg
      661 agattcaagt cgaccttcta acccgcaacc ctgccccgcg gcgcgacatc ggacaggggt
      721 gttgccgggc gggccgcttg ccgttatttc ggcggctcaa aaaactgcgg gctcgggggt
      781 catcgtgatg tcctggttgt gggccgtgtt cacggtcctg gctgccggcg ggcaggtctt
      841 gcgcaatgcg cagcagaaag aattgactgg cacgctcggc accgtcgggg ccacgcacgt
      901 tcgcttcctt ttcggtttgc cctttggcct cctgtttctg gccttggtgc tgctggcgac
      961 ggggcagggc ctgccgccgc tcaacgccgc catgttggcc tggagtgcgg ccgccgcgtt
     1021 ggcccaaatc agcgccactg ccctgctgct cgccgccatg cgcgaccgct ctttcgtggt
     1081 caccacggcc tacgccaaga ccgagccggt gcaggtcgcc attttcggcc tggcgttcct
     1141 cggcgatcgg ctgtcgcccg gcctggcgct ggcgatcggc attgccacgg cgggtgtgct
     1201 gatcatgtct tggccgcgcc gtagcgcgga ggaggccttc agctggcatc cagcgctcct
     1261 cggcatcgcc tcgggcgcca tgttcgccat tgctgccatc ggctttcgcg gcggcatccg
     1321 ggcgctgaat gcccccaatt tcgtgctggc tgccaccacc acgctggcct tcggtctcat
     1381 catccagacg gcgctgctct ccgcctacct ggcgctcttc gaccgtgcca agctcatcgc
     1441 catttgccgt gagtggcgac cctcgatgct ggccggcttc actggggcct tcgcctcgca
     1501 gatgtggttt ttggcctttg ccctcgagag cgcggccaag gttcgcaccc tggcgctcgt
     1561 cgaggtgtta tttgcccaga tcctctcgcg caatcttttc aaacagcggc tggcctcgcg
     1621 cgaggccttg ggcatcctcc tgatcgtcgc aggcgtcgtt ttgctcttga atctttgagg
     1681 ctcagtgctc agctgagcgg catggacccg cagaggatgg caccccggga aatgggcacg
     1741 caggcgccgc cgcgagatgc ggcgcgcgag gcggcgcggc tcgagaacgc gcgccggcgc
     1801 ctcgcagccc tcaagggctt ttacgtccac ctgatcgtgt tcgtcctcgt catcgccgga
     1861 ctgctcatta tcaacagcct caccggcggc ccctggtggg ttgccttggt gttctttgcc
     1921 tgggggctcg gtgtgctggc gcacggcctg gcgctcgtgg cgcgtcgctc gcgcgcgatt
     1981 gcggcctggg agcagcgcaa gcttgcggcc tatctggccg aggatagcgg cggcccgcca
     2041 gggagccgcc acgatcccaa ccggcctatt tgatcagacc ctctgcctgc atggccgctt
     2101 gtaccgcggc gcgggccccg acccgcttgc gataggccaa aacgttggca aagcccgaga
     2161 ggtcgacgcc cgtgtgagcg gcccagttcg acaccgtgaa caaataggcg tcggcaacgg
     2221 aaaaggcctt gcccatcaag tagtcgttgt ctgccaaatg cttatccagg tgttgaaagc
     2281 gcgtggcgag ccgttcgcgg aagacctgct tggccgcgtc gggcatgttg gggttaaata
     2341 agggtgagaa gcccttgtgc agctcggtgc tgatgaaatt gagccaggac tgcagcttgg
     2401 tgcgctccaa gctgccgctt gccggcgcca gctgcttgtc cggtaccttg tcggcgatcc
     2461 attgcacgat cgccggcccc tcggtgagga tggtgccatc atcgagctcg agagcgggca
     2521 cgtagcccag gggattgatg gagcggtagt caccaccctc catcgccttg cttttggtat
     2581 cgaccttgac tttgtcgaac ttcaggcccg cttcgttgag gacgatgtga ggtgatagcg
     2641 agcaggcacc gggcgcataa aaaagcttca ttagacgtct cctacttgac gcggccgcac
     2701 gaggcgatat gcatgcattt gcatttattg cacttgcata caagtcgcga tctgccgagg
     2761 caaggcgcct cccttagagt tgatcgatcg aggctgacag ttgatcgatg caggctaagc
     2821 cgacaaccgg ggtcctcaag gcttggatca gcttgctgcg cacggagcgc gagctggtcg
     2881 acaaggtcga ggagtgcttg aagcgcgccg ggctgccgcc gctcgcgtgc tatcacgtgc
     2941 tcgatgcggt cgagcgcgcg cccaagggca tgctgcgtca agcgcaagtc caggcgggca
     3001 cgcagctggc ccagtatacg ctttgccgcc tgctggaccg gctggagcgt gacggcctga
     3061 tggagcgcca ccagtgccaa cgggacggcc gcaacaacgt gctcttgctc acctccaagg
     3121 gccgggctat gcgccgcagc atctggcccg tgtacgccaa ggcgatcgag gagcattttg
     3181 gcagccgcct cagctcagct gaggctgagc aattggcgag cctgttgggc aagctcggct
     3241 gaccgccgaa gcggcatccc ttcgggcacc tggaccccga tggccgttgg tgtaggatgc
     3301 ggtgcttcgg gccgttccgg cgcccatcgg cgcgtttctt tagagggata ttgggagatg
     3361 gcgggacctg ccgcgcaggc cgacagtccg gctggcacgc tgatgcaggg caaacgcggc
     3421 ctcattatgg gcgtcgccaa taatcgctcg attgcctggg gcattgccaa agcgacggcc
     3481 gcgcagggcg ccaagctcgc cttcacctac cagggagagg ccctgaagaa gcgggtcgag
     3541 ccgctggcgg cggaggcggg ctccgaaatc gtcctgccct gcgatgtcgc cgacgcgggt
     3601 tcaatggacg cggtctttgc cgagctcggc cgacgctggg gggagcttga tttcctggtg
     3661 cacgccattg ccttttccga caaggccgag ctcgatggcc gctacatcga caccagtgcc
     3721 aagaacttca accagacgat gctgatttcc tgctactcgt ttaccgcctt ggcccagcgc
     3781 gccgaaaagc tgatgcgcca gggcggcgcg ctgctcacgc tcacctacta cggggccgaa
     3841 aaggtcatgc cgcattacaa cgtgatgggc gtggcgaagg cggcgttgga ggcgagcgtg
     3901 cgctacctgg ccgccgatct cggcaagggc ggcattcgcg tcaacgccat ttccgccggt
     3961 cccatcaaaa ctttggcggc ctccggcatc gccgacttcc gctacatttt gaaatggaat
     4021 gagtacaact cggcgctgcg ccgcaccgtg accattgagg aggtcggcgc ggccggcctt
     4081 tatctattgt ccgatctctc gcgcggcgtc accggcgagg tccatcatgt ggacgcgggc
     4141 taccacgtcc aaggcatgaa gaacgaagag gctcctgaca tttccgtggt gcgagacacc
     4201 acttgagcga aacgccgcgc gagagggtgg cttagcgcag atccggtggg ctggtgcgcg
     4261 catcgacgca acgcacgccg ttccaataga agtactcgcc gcaacccccg cgccgtcccg
     4321 gcacacgcac gaccacgtcg gggcgcgcgt catagtcata ggtgctggtg tagccgtaga
     4381 cccgcgcgcg cggtggcggc ggcgcgtagt cgtagtcgtc gtagtagccg gacgggccga
     4441 cgtagacgcc aaatcccacc tgcgccgaag cagagttggc tgcggcgagc gatgccagcg
     4501 ccgcggctgc cgccgtgatg attgcaaaac gcatgtgacc tcctgaatgg tcgtttgata
     4561 ggcgaactga cgtttaccaa cccaaaccag ccagctttgg aatcgttccc ggcacgcggg
     4621 cgcggcctgc gccacaaaac cgccatgccc gacgcctcat ctcgcgagcg cgacccgcgg
     4681 gggcgaggcg cacggggaga agcaatgcgt gccgtaacgg ctttaatcat cacgctgatt
     4741 gcgctcggcg caccggcaac ggcggcggca ccaacaaaca aggcgaccgc cgcggcgaag
     4801 aagggagaat tttcctcctc ggaaagcatt ctgcgctgga tcaacggcta ccgcctcaag
     4861 ccggagcccg ccaaggcgcc cgcggcagca cgcgccatga gcgagctcaa cctcttccgc
     4921 gatctggaaa cggccggcgt ttacgtcggc tttctggctg gcgtgctgga gaccaatccc
     4981 aagaaggcgg aagagctgat cgctaagatg tttccgatgc cgccggaaga tcaggtcgtg
     5041 atcgtacgcg ccatcgccta ttccaacctg cccgagtgga aggctctgct gctcaagttt
     5101 gccgagcgca tgccggcccg ccagggtttg atcgaccgct tcgtgcacga caagatgccg
     5161 acgctcaagc agatcccatt ggactccggg tcggcgccgc tcgacatgct gtggggcaaa
     5221 tacttcgcca cgggttccta cgagccggtc atgcgcatcg tctccatcct cgaatggtca
     5281 aaggacgcca acaacgtcga tcgcttgacg gtcgggtcga tggcgaaatt gacgctcgcc
     5341 accaatgcct cgcgtgacaa agagctcatg gacatgctga aggtctccat gaagcacgag
     5401 accaaggaaa accgcgccgt cttgcaggag gtgatcgacg ccgcggaaac cttcgagttc
     5461 ggccgcgtgc gcaaggaagc catgggcgcc atcgaccaac tgaaaatcaa gggacccgcc
     5521 tcggcgcgca atgtgcagtg gtgggggacg gcggggcaaa ccgcgctcgc gctcggttgt
     5581 atcgtggcag gagcgctggg gcacgtggaa atcggcattc cctgcgtggt tgggggcgct
     5641 gtttccggcg ccgcactcaa gtacatgacg ccgcagtaag gcgaatgccg tcctggctcc
     5701 taggacgcct tctgctcctg ttcggctctg gcctgttcgg ccgcatcctg ggcaaacaga
     5761 tcttccagct cctgcgccga ggtgcgcgcg cgtgcctgca gcctctcgac gtccgtgtag
     5821 tggcgatagt cctcgtaaag ccggcgccgg tcgtgcgcct tgaaggtttc aaccacgaag
     5881 ctcacctcac gctgcgagag gccgaagcca cgcaggagat cccgggtcag ctcgatcgag
     5941 gacaggaagg tttcgcgccg gatcgccgtc acgccgagat ccatcagccg atgcgtgtgt
     6001 cggcggtcgc gggcacgggc gtagatcggc acgtgcgggt agtgctggcg caccatttgg
     6061 gcaatgcgca ccgaagcctc gacgttgtcg acggcaagca cgaaggcgcg ggccttgtcg
     6121 gtctgcgcgg cttcaaggat ctcgtggcga ctggcatcac cgtagtagat cttggccccg
     6181 aatttgcgca cgaccgcgac ctgctcggga ctggagtcga gggccgtgat ggcaaacttt
     6241 ttggccgtca gaatacgggc cacgatctgc ccgacgcgac cgaatccggc gatcacgaca
     6301 tgcccgtcgt ttgcaggtat ggcatcgaag gctcgggcga cgacaggttt cggcaaaagc
     6361 gtctcctcca gcagcaacaa cagcggggtt gccgccatcg acagcgtcac ggcgaccgac
     6421 aagagatcgg ctacggagcg ctccagcacg cccgaggcca tgccgctggc aaagagcacg
     6481 aaggcaaact ccccgccctg ggaaatcgcc aacgcgaacc gccgcgcggg gccggtttcc
     6541 agacccgcgc gccgacccag gccgtagagg atcagaccct tgatggcgag caggcccgcc
     6601 accacccagg cgacgagcca agggctttgg ccgatcaaac cgaggttcag cgacatgccg
     6661 accgcggtga agaagaggcc gagcagcaag ccctcgaagg gcgcgatgtc ggcctcgatc
     6721 tcgtgccgat actgcgagtc ggcgagcaac acgccggcga tgaaggcccc cagcgaggcg
     6781 gacagcccgg cctgttccat caccagcgtg acgcccacca ccgtcaacaa agcgctggcg
     6841 gtcatcgcct ccttgacgcg tgtgcgtgcg acaaggctga aaatgccgtc gagaaggtag
     6901 cgcccgatta ccacgaccag cacgatcacg ccgatggcct tggccgccga cagcagttcc
     6961 atttgctggg ttgcgtcctt gaccgagacg gcgaacaagg ccggcaccgc gatcagggga
     7021 atggccgcca aatcttggaa cagcagaacc gcaaaggcga gccggccatg ccgtagcgcc
     7081 agctcgccct tctcctccaa aatttgcagg gcgaaggcgg tcgaggagag cgacaaggcc
     7141 agcccggcga aaaccgcggc ttgccaggcg agcccgaacg ccatggccac aaacgccagc
     7201 gccagccccg tcagggcgac ttgggcgcca ccgaggccga agatggccgc gcgcatcgcc
     7261 cacaagcgtt tgggtctgag ttccagtccg atcaagaaca acaacagcac cacgccaaat
     7321 tcggcaaagt gcaagaccgt ggtgacctcg tacacggtga ccacaaagcc gagcccgaac
     7381 ggaccgatca ggaccccgac caacagatag cccagcacgt tgccgatgcg tagcagtttg
     7441 gcgagcggcg ccgccaccac cgccgcggcc aaaaacaagg caacctgggt caacgtcaca
     7501 tcgctcatcg gcttgggccc aaagctgggg agaagaggtg ataggaaggc atgcccagcg
     7561 aacgcaaccg caaaatgacg ggcgcgcggc gtccaggggc acgagcgccg gcgctccgca
     7621 aggaccaacg cccccgcgcc gtctgttggc taagccgcgc ggcgcgacgc cgccggcatt
     7681 cgccccgttc gcagaccctc acctctcaag aggagtactt gatggtacag ccatagggtc
     7741 gcgaggtttt gacggacacc gggctgccca gagccagctc gcccagcgcc gcgtccacga
     7801 aattcttggc cgttttcagg tcctccaagc gcgtggtcgg cttgtcgtcg atgccgccca
     7861 tgtaggccaa ggcgccatcc cccgtaatga catacatatg cggggtcacc tgggccccat
     7921 agctgcggcc cacggagccg ttggggtcca acagcaccgc cgtgggcgcc gccccgcggt
     7981 ctgcggtgag cttgttggct tccagcccat gcacgaagcc ttgtgcccca gggcgcgagg
     8041 agattagggt caaccaggcg acgccctgcg ccgtccattt cttctgcagc gcctgcatgt
     8101 tctggctgcc atagtgcttg cgcacatagg ggcagtcgtg attggtccat tccagcacga
     8161 tggtcttgcc tttgaagtcg gctagcgaga cgctacggcc gttgctatcc gtcagggtga
     8221 agtcgggcgc gggcgcgccg agacgcgcct gggcggacgg gtcggccata gcccccgcgg
     8281 cagcgaggag gccgatgagg ccggcggcgc cgagaagcgc aatcaagtca cgacggcaga
     8341 catcgaacat tgagttcctc cggctcgggg gcaacgggat tgcgacggcc ggcacgatag
     8401 cagtcggggg cggcgggaca caaacgcggg cgaaacgccc gcaacgccga ggccgcgcgt
     8461 ggtcgcatcg cccgccctcg attgcgctcg gccagtgcgc cccacgccct gcctggccgg
     8521 ggctcgcagg cgagcgcgca gcgctacccg ggcgcagcgc gcaagttcca cccgtcaacc
     8581 ccaagcggct cagcggatcg acgcccgagc ctgctgcctc agaccggaca cgcccctcgc
     8641 tggcccccct ctgagcaaca cctgatcggc gcgctccaag gcttcacacg attcttgcag
     8701 caggtgcagg gactttcgca ctctcgcgag agcggcgccg ttagcgcgaa gcgcaatgat
     8761 aacgttggcg gccgcgcgcg gcctctctca cggcgagcat ctgcaatccg gttggcctat
     8821 atgagcctgg aaagtgcgtc gacagaatgc caaccaaagc gccgttggtg gagagcaatg
     8881 gtgtggactg catgcctcta atgccggcac gacgaacgat gtcccggcat gcgccgagct
     8941 gtgggtctcg catgacgtct tcaattacaa tcgattggcg cttgcgcaat gcgcgggcgc
     9001 aggcgcacgt gctggaaagt tgacgtattc aaaaaaattc aagaactcgt cgcgaaacct
     9061 tgctgcgctt tgatctgcag ctgacccgtt tcccagttga tcagttgcac gttgccgaag
     9121 tcacactggc acacctcgag cgcgcgctgc agcaggtaaa gggcgaccgc ttgcacatcg
     9181 gcgcatcggc gcagacggtg gaccagcgcc tccacggcgg taccccaatg gtgttagccg
     9241 gtctcagcct atcagcaagg ccgaaccggc aacttggcca gggttaaaac agaaagcctc
     9301 catgctctcg cgcttccgcc gtggcgaggc tttgcctcga tcagccggcg cgcgcctcct
     9361 tgggcgggtg agcagccccg cagagcccct cttggccgct gcgcctgtcg gacgtcagcg
     9421 caggcgatcg ggacgggtta agcgcgagcg gccggcctga gcctcccgcg acgtcccggc
     9481 gcttgatctg cgccggccag gctgcctgcc gcagcgtgcc cgcaccacgg gcccctcaga
     9541 tcgaccgggg tgtctcgggc attacaaagg gatggcaagt ctcgaaggcc cggctcgcgc
     9601 gcagcaccag atcatcgcgg ccgatcgcgc ccaccacctg caagccgacc ggcagaccct
     9661 cccgcgtgaa cccgcacggc agcgacgccg ccggttgctg cgtcaaattg aaggggtagc
     9721 taaagggcgc ccagccggtc cagtcttcgc cccacgcccc cgccggcggc agcaaatgcg
     9781 ccacctcgaa ggcggcgagc ggtaaggtgg gcgtcaacaa taaatcgtag ctggcaaaga
     9841 agcgcgccat acgatggtgc agctccgcgc gggtcagcgc tgccccgatg agctccacgg
     9901 ccttgatgtg ccgacctcgc tccgcggccg ccaccagccc gggatcagag acggccgcag
     9961 gatctccctg caacagggcc agctgatagg ccatgccggg ccacca
//
LOCUS       DASTMT010000184         1522 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_429009, whole genome shotgun sequence.
ACCESSION   DASTMT010000184 DASTMT010000000
VERSION     DASTMT010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 1522)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1522)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1522
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_429009"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..704)
                     /gene="paaA"
                     /locus_tag="VFR00_04135"
     CDS             complement(<1..704)
                     /gene="paaA"
                     /locus_tag="VFR00_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018648122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaA"
                     /protein_id="HEU0158474.1"
                     /translation="MYTQGLNSTEAATGGQSEDPARLLRFQAKVDSEDKIEPNDWMPE
                     GYRRTLLRQISQHAHSEVIGMQPEGNWITRAPSLRRKAGLLAKVQDEAGHGLYLYAAA
                     ETLGMAREEMVDQLLTGKAKYSSIFNYPSLSWADIGAIGWLVDGAAIMNQIPLCRCSF
                     GPYARAMIRICKEESFHQRQGYEIMVTLCNGTAAQKNMAQDALNRFWWPVLMMFGPSD
                     KESLHSADTMKWKIKR"
     gene            complement(857..1339)
                     /locus_tag="VFR00_04140"
     CDS             complement(857..1339)
                     /locus_tag="VFR00_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016846811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HEU0158475.1"
                     /translation="MDGIEAAAAAIAARLKARGETLAVAESSAGGLISAALLAVPGAS
                     AYFLGGAVVYTRQARRGLLAISDEAMSGIRSASEPYAVLLARTVRERMGASWGLAETG
                     ATGPSGNRYGDPAGHACVAVDGPTRRVLTLATGSADREANMQTFTQRALATLLEELGR
                     "
BASE COUNT          226 a          517 c          500 g          279 t
ORIGIN      
        1 aaacgcttga tcttccactt catggtgtcg gcggaatgca ggctctcctt gtcggagggc
       61 ccaaacatca tcaagacggg ccaccagaag cggttgagcg catcctgggc catgtttttc
      121 tgcgccgccg tgccgttaca cagcgtgacc atgatctcgt agccctggcg ctggtggaaa
      181 ctctcctcct tgcagatgcg gatcattgcc cgggcatagg ggccgaagct gcagcggcag
      241 agcgggatct ggttcatgat ggcagcgcca tcgacgagcc agccgatggc gccgatatcg
      301 gcccacgaca ggctcggata gttgaagatc gaggagtatt tggctttacc cgtgagcagc
      361 tgatcgacca tctcttcgcg tgccatgccg agcgtttctg ccgccgcgta gaggtagaga
      421 ccgtgtcccg cctcatcctg caccttggca agcagcccgg ctttgcggcg caacgagggg
      481 gcccgcgtga tccagttgcc ttcgggctgc atgccgatca cctcggaatg cgcgtgctgg
      541 gagatttgcc gcagcaaggt gcgccggtag ccctcgggca tccaatcgtt gggctcgatt
      601 ttgtcctcac tgtcgacctt ggcttggaag cgcagcagcc gtgcggggtc ttcgctctgc
      661 ccgccggtgg ccgcctccgt gctgttgagg ccctgcgtat acatcgatcg tgacctttgg
      721 cgaattggcc cgaagcacca gtatgccaca ggtagcggta cgggcgcgac cccggggccg
      781 cgcgggatcc ccgcgccgcg gcgcgcgccg ttcggccttg ggcaggcttt tttagctttc
      841 acctcgggga gcgggctcag cgcccgagct cttccagaag cgttgccaac gcccgttggg
      901 tgaaggtctg catgttggcc tcccggtcgg cgctgccggt tgcaagcgtc aggacgcggc
      961 gcgtgggacc atcgaccgcc acacaggcgt gcccagccgg gtcgccgtag cggttgccgc
     1021 tgggaccggt ggcgccggtc tcggcgaggc cccagctggc acccatccgc tcgcgcaccg
     1081 tccgcgccag caggaccgcg tagggctcgc tcgccgagcg gatgccgctc atggcctcgt
     1141 cggaaatcgc caacagcccc cggcgcgcct gacgcgtata gacgacggcg ccccccaaga
     1201 agtaagcgga ggcgcccggc acggccaaga gcgccgccga aatcaaccca ccggctgagg
     1261 attccgcaac tgcaagcgtc tccccgcgcg ctttgagcct ggccgcgatt gccgcggcgg
     1321 ccgcctcaat cccatccatg ccttcgcctc cgtgcttggg tttttcgcgt tggcggccgc
     1381 gcctccagct ggcgcggttg gctcctggcg cctgcccggc ggggctgccc cggttgagcg
     1441 ctgcaggctt tgcgcctatt tttgccagca tgcaagacaa ccccatccac gtcatcggcg
     1501 gcgggctcgc cggcagcgag gc
//
LOCUS       DASTMT010000185         3103 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_2723709, whole genome shotgun sequence.
ACCESSION   DASTMT010000185 DASTMT010000000
VERSION     DASTMT010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3103
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2723709"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            135..404
                     /locus_tag="VFR00_04145"
     CDS             135..404
                     /locus_tag="VFR00_04145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0158476.1"
                     /translation="MPAGHGRHTAELLLARSAAAQGLPGGHGGHAAELLPADMPGRHR
                     WYTAQLPSAVLPSGHGGHAAELLPADMPRRHGWHTAQLPSAAIAA"
     gene            604..1773
                     /locus_tag="VFR00_04150"
     CDS             604..1773
                     /locus_tag="VFR00_04150"
                     /inference="COORDINATES: protein motif:HMM:NF012725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU0158477.1"
                     /translation="MLRATAMVLGLMAMPAFIVPVMGQEKRPPHPQGCFAQRIDQPAD
                     LQDRMAACLRVIGDSSVSADKRAEAYLRHALARAQQIALSNSNDGIDRALAALAGSRP
                     IQSNPELQKSIHETRARLYLRKADLDRAIVEYTAWLGLEPNSAAAYAQRGMAYRTLGK
                     VGEAIADLRKALSLEPSNAAVKEELRLTEVSAPTVGKAEDKGPDANKIGVLKAELKQA
                     QEAAKAMEDRLAALEAAKAAKPAPAPPTDVKPAAPAGPDAAKLAGDLQSQLKRVGCYP
                     GAVDAQWGKMSQLALQRFAQHAALALPLGEPTTAALEAVSARPDRVCPLACEGNETEV
                     NGTCVARRTRTKGKRSHASRRGERSPTNSSGRPSGGPSVGITIGVGRRGGIGIGF"
     gene            1794..2717
                     /locus_tag="VFR00_04155"
     CDS             1794..2717
                     /locus_tag="VFR00_04155"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU0158478.1"
                     /translation="MLRRTASGFTTFVLLAGSTTALADDLADCSQRADLDLRVRGCTR
                     MIAQTKDKQSLTAFYGLRGVAFSAKGDHERAIADHTKAIEINPTAATPHYRRGLARAR
                     KREFDRAIADFNNAIDINPRLALAYYNRGGSFGRKGDLDRAIVDYTKAIEISPLFTLA
                     HFVRAGAYHKKRDYDAAIAGYTKVLEIDPKAAQTYHLRGVAYQRKGDHDRAIVDYTKA
                     IELDDRSFAAFFARGLAHRHTGDLNRSIADLTKAMQIDRESAQAVSNRGFSFERKGAK
                     DRAVADYRNALRLDATNKQSRDGLKRLGASP"
BASE COUNT          572 a         1050 c          999 g          482 t
ORIGIN      
        1 cggctgaacg tgcgggcgac ctgtaggtgg cggttccctg accgtcctcc tcccaccgag
       61 ttcgacccgc gtcgcccggc gccattgccg aaactaccac catcccacac gccgccgccg
      121 cccaccatca tctcttgccc gcagggcatg gccggcacac cgccgaactg ctattggccc
      181 gctccgccgc cgcccagggc ctgcccggcg ggcatggcgg gcacgccgcc gaactgttac
      241 cggccgacat gcccggcagg caccgttggt acaccgccca actgccgtcg gccgtcctgc
      301 ccagcgggca tggcgggcac gccgccgaac tgttaccggc cgacatgccc cgcaggcatg
      361 gttggcacac cgcccaactg ccgtcggccg ccatcgccgc ctaggccgcc gccgccgcag
      421 tgcccgatgg tgcgggtctg cgtgcaatac ggcccggggc gaccgggcag ctttaaccgc
      481 ccctgcatcc gctacgagat gcggcgccag tgccgaagcg gcgacgtcag atgattggcc
      541 agagaatcaa agatgtaacg ttcaaccagc ttggctcccg gttggggggc agtgaggtct
      601 gcgatgcttc gcgcaacggc catggtcttg ggtttgatgg ctatgccggc gttcattgtg
      661 ccggtcatgg gtcaggagaa gcgtcctcct cacccgcaag gctgcttcgc tcagcgcatc
      721 gatcagcccg ccgatctgca ggaccggatg gccgcctgct tgcgtgtcat cggtgatagc
      781 agcgtgagtg cggacaagcg tgccgaggcc tatctgcgcc atgccttggc gcgtgcccaa
      841 cagatcgcgc tttccaacag caacgacggc attgatcgtg cgcttgccgc cctcgccggg
      901 agtcgaccga tccaaagcaa tcccgagttg caaaagtcta tccacgagac ccgcgcccgc
      961 ctctacctgc gcaaggctga cttggatcgc gcgatcgtcg agtatactgc ctggctcggt
     1021 ctagagccca actcggccgc ggcctacgcc cagcggggca tggcctaccg gacgctcggc
     1081 aaggtcggcg aggccatcgc cgacctgcgc aaagcgctct ccctcgagcc gtccaatgcc
     1141 gcggtcaagg aggagctcag gctcacggag gtgtcggccc cgacggtggg caaggcggaa
     1201 gacaagggtc cggacgccaa caagattggg gtgctcaagg ccgagctgaa gcaggcccag
     1261 gaggcggcca aggcgatgga ggatcgcttg gcggcactcg aagccgccaa ggccgcaaag
     1321 ccggcacccg ccccgccgac ggacgtcaag ccggcggctc ccgcgggccc cgatgctgcc
     1381 aagctggcag gcgacctgca gagccaactt aagcgcgtcg gctgctatcc aggcgcagtc
     1441 gatgcgcagt ggggcaagat gagccagctg gcgctgcaga ggtttgcaca gcacgcggcc
     1501 ttggcactcc ccttgggaga gccgacgaca gcggccctgg aggccgtgtc agcgcgcccg
     1561 gaccgcgttt gcccgttggc gtgtgagggc aacgaaacgg aggtcaacgg cacttgtgtg
     1621 gccaggcgga ccaggacgaa gggcaagcga tcccacgcct cgcgcagggg cgagcgctca
     1681 ccgacgaact cgtctggacg accttccggc ggcccgtcgg ttggcatcac cattggggtc
     1741 ggcaggcgtg gcggtatcgg catcggcttc tgagacggaa gggagacgtg gcaatgctaa
     1801 ggcgcaccgc atcgggtttc acaaccttcg tgcttctcgc gggcagcacc actgcgctgg
     1861 cggacgatct tgccgattgc agccaacgag ccgatctcga cctgagagta cgtggctgca
     1921 cgcgcatgat cgcacaaacg aaggacaagc aatcgctgac ggcgttctac gggttgcgcg
     1981 gggtcgcctt cagcgccaaa ggcgaccacg agcgcgccat cgccgaccat accaaggcca
     2041 tcgagatcaa cccgaccgcc gcgacgcccc actaccgccg cggcctggct cgcgcacgca
     2101 agcgcgagtt tgatcgggcg atcgccgatt tcaacaatgc catcgacatt aatccgaggc
     2161 tggccctggc ctactacaat cgcggcggct ccttcggtcg caagggcgac ctcgatcgag
     2221 cgatcgtcga ctacaccaag gccatcgaga tcagccctct gtttacgctt gctcatttcg
     2281 tgcgcgccgg tgcctaccac aagaagcgcg actatgacgc ggcgattgcc ggctacacca
     2341 aggtgctcga gattgatcca aaggccgcac agacctacca tttgcgcggc gtcgcctacc
     2401 aaaggaaggg cgaccacgat cgggctattg tcgactacac caaggccatc gagctcgatg
     2461 accgttcttt cgccgccttc tttgcgcgcg gccttgccca tcgccacacg ggcgacctga
     2521 accggtcgat tgccgacctc accaaggcga tgcagattga tcgcgagagc gcccaggctg
     2581 tctccaatcg cggcttcagc ttcgagagga agggggcaaa ggatcgggcg gtcgccgact
     2641 accgcaatgc gttgcgactc gatgccacca acaagcaaag ccgagacggc ttgaagcgcc
     2701 ttggagcatc gccgtgaagt cgccctgcat ttcgcacttc gccgcaagaa cgaacgtcac
     2761 gcgctcgtgc gctatgccga tgtcatcaac gtctatgcgt ggccaggcag gccaggacga
     2821 gggcaagcga tcctatgctt cgcacaggga gcgagcgctc atcgacgacc tcgcctgggg
     2881 ggacgcgccg gcggcccgtc agttggcatc acaattgggg tcggccgtcg ccggcgggat
     2941 cggcatcggc tcttgagacg gaagggagga gaccgcattg tggcttcgga tccctgcaac
     3001 attggcaggg cgggcgtcca accgcgacaa ccaacgcgcg tctgggcctg gcgatgcgat
     3061 ggaccggcaa cccactgagc tgggcggaag cgatcagacg ctc
//
LOCUS       DASTMT010000186         1512 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_3053263, whole genome shotgun sequence.
ACCESSION   DASTMT010000186 DASTMT010000000
VERSION     DASTMT010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 1512)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1512)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1512
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3053263"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            470..>1512
                     /locus_tag="VFR00_04160"
     CDS             470..>1512
                     /locus_tag="VFR00_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013949473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEU0158479.1"
                     /translation="MLNAPRRAGRHFLQIPGPTPVPDRILRAMDMPVIDHRGPEFKAL
                     GLRVLAGIKSIFKTSEHVFIYPSSGTGAWEAALVNTLSPGDKVLMYETGHFASLWHKL
                     ALKLGLEPELIGSDWRSGVDAEAIEARLRQDKAHAIKAVCVVHNETSTGATSRIEEVR
                     KAIDAAKHPALFMVDTISSLASIDYRHDAWGVDVTVGGSQKGLMLPPGLGFNAVSQKA
                     LAAARAASQPKSYFGWEEMLPANKDGFFPYTPATNLLYGLAEAIDMLHEEGLERVFAR
                     HDRHAEATRRAVRAWGLEIWCKDAKHYSSSLTAVLLPGGEDADALRAVALEAFDISLG
                     TGLSKLAKKVFRIG"
BASE COUNT          253 a          488 c          511 g          260 t
ORIGIN      
        1 gcacgtcatc gtccctcttg ggaaccacga cgccgatcgg ctcgatttgg taatgggagg
       61 cgtcggtggc gtagcgaccg cgcgaaaagc ggtcgaagag cacctcgccc tcgagttcac
      121 gctcgagccg tgcggcaagc cggtgcgaga tcggggccgg gttcatgacg ggcatgggcc
      181 cggtaatgcc cgtcccgggc cgcgcgcaca agagggatcg ggcgcttggc tggccgccac
      241 gggaggagaa gcaaacgcgc cgcgcaggcg ccaagccgtt gagcggcgcg cggcgccgcc
      301 ctcgcaaggg ggagaggacc gtgccgaccc ttgccgccag aaggatagag cccttcgggg
      361 cttgccggcc ttggtctgcc acggctggcg tcggcattgc cgcaacgggt tggcagggcg
      421 ctgcgagcct gctttaagct aagggcgccc ttgtcgatgg agagctcgca tgttgaacgc
      481 gccgcgccgt gccggccgcc attttctgca gattcccggc ccgaccccgg tgccggatcg
      541 tattttgcgc gccatggaca tgccggtgat cgatcatcgc gggcccgaat tcaaggccct
      601 gggcttgagg gttttggccg gcatcaagtc gatcttcaag acctccgagc acgtcttcat
      661 ttacccctct tccggcaccg gtgcctggga ggccgcgctc gtcaatacgc tctcgcccgg
      721 cgacaaggtc ctgatgtacg agaccgggca tttcgccagc ctgtggcaca agcttgcctt
      781 gaaactagga cttgagccgg agctcattgg ctcggactgg cgcagcggcg ttgacgccga
      841 ggcgatcgag gcccgcctgc gccaggacaa ggcgcacgcg atcaaggccg tctgcgtggt
      901 ccacaacgaa acgtccacgg gtgccacctc gcgcatcgag gaggtgcgca aagcgatcga
      961 tgctgccaag catcccgccc tcttcatggt cgacaccatc tcgtcgctgg catcgatcga
     1021 ctatcgacat gatgcgtggg gcgtcgatgt cacggttggc ggctcccaaa agggcctgat
     1081 gctgccgccc gggcttggct tcaacgccgt cagtcagaag gcgcttgccg cggcgcgcgc
     1141 ggcttcccag ccgaagtcct actttggctg ggaggagatg ctgcccgcca acaaggacgg
     1201 gttttttccc tatacgccgg ccaccaacct gctctacggc ctggcggagg ccatcgacat
     1261 gctgcacgag gagggattgg agcgcgtatt tgcgcgccac gaccgccacg ccgaggcgac
     1321 ccgccgggcc gtgcgagcct ggggactcga gatttggtgc aaggacgcca agcattactc
     1381 ctcgagtctg acggccgtgt tgctgccggg cggtgaggac gccgacgccc tgcgcgcggt
     1441 cgcgctcgaa gcgttcgaca tttcgctcgg caccgggctc agcaaactgg caaagaaagt
     1501 gtttcgcatc gg
//
LOCUS       DASTMT010000187        15386 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_3053311, whole genome shotgun sequence.
ACCESSION   DASTMT010000187 DASTMT010000000
VERSION     DASTMT010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 15386)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15386)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15386
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3053311"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..656
                     /locus_tag="VFR00_04165"
     CDS             <1..656
                     /locus_tag="VFR00_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ADP/ATP-dependent (S)-NAD(P)H-hydrate
                     dehydratase"
                     /protein_id="HEU0158480.1"
                     /translation="LAHFLSDRRRNVVLIGPGAGVGRATATSVLGVLASPAAVVLDAD
                     ALTSFAANAEAGELRAVGFGFVVRDPEPAPEAKALFAAIKARPAPVVLTPHAGEFARL
                     FGELPGSKLDYARKAAELSGAVLLLKGADTVVAAPDGRAAINDNAPPWLATAGSGDVL
                     AGFVGGLLGQGMPAFEAACAAVWLHGAAAQAFGPGLIAEDLPETLPQVLARLSFVGT"
     gene            774..1616
                     /locus_tag="VFR00_04170"
     CDS             774..1616
                     /locus_tag="VFR00_04170"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019995478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0158481.1"
                     /translation="MNDEPTARAAPARAAVASAIELRNLAVAFTLPSGSSYQAVAATN
                     LNVGAREFVAVVGPTGCGKSTLLNVAAGLLPPSAGEAFIFGAPLLGLNQSAGYLLQQD
                     ALMPWKTARDNVAIGLEVGGTPRREALPAADGWLKNVGLAAFGDRYPHQLSGGQKKRV
                     GLAQVLIRDPKILLMDEPFGPLDAQTRQIMGTLLLELWAKDRKAVLFITHDLEEAIAL
                     ADRVLIMSAGPEAGIIGDYSIDLGRPRDASEIRLLPQFHALHKAIWSQLKDEVMRAYA
                     GGGA"
     gene            1619..2485
                     /locus_tag="VFR00_04175"
     CDS             1619..2485
                     /locus_tag="VFR00_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013503155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0158482.1"
                     /translation="MSRAGLLSLQVLVAVLIIGIWQLGSTVSVGGWYLFPKFFFSTPG
                     EVALRVWSLFADGTAKIVTCPEPIDISRILVVSDPCFGKTIWQHLLITLTEGLLAFVI
                     GAVSGVALGFWLARNPLLSAIFDPYIKMLNALPRVVLAPIFMLWLGLGIWSKVALGVT
                     LVFFIVFFNVYQGVKEVSPVILANARMLGMNPRQLFVHVYWPSALSWMFSSLHTSVGF
                     AIIGAVVGEYLGSAAGLGYLIQQAEGTFDTTGVFAGMVILAVFVLIIDWLVTIIENRL
                     LVWRPTGSGARA"
     gene            2510..3529
                     /locus_tag="VFR00_04180"
     CDS             2510..3529
                     /locus_tag="VFR00_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008121060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0158483.1"
                     /translation="MSCRKFLIIAVLALLGMGRAEAQAPERKDVRLGVGGAPALYYLP
                     LALTERLGYFKEQGLNVEINDFKGGSQSLTALIGGSADVVTGAYEHTLRMQGKGQDVV
                     AIIELGRYPGISLAVKKDRADKIKTVADLKGAKIGVTAPGSSTNMIVWYLMAKAGLKP
                     DDASFIGVGTGAGAVAAIQRGEIDAISNIDPVMAKLESTGDIVTLAETRTTEGTAKVF
                     GGPMSAAVLYAKRDWLEKNPNTAQALTNAFYKTLKWMRDATPEAIAATVPQEYWLGDK
                     ALYTAAVKANMQVYSQDGVVSAESRQRSLDFLKQFDKEIAGATIDSAKTWDDRFVKKA
                     AATVR"
     gene            3576..4664
                     /locus_tag="VFR00_04185"
     CDS             3576..4664
                     /locus_tag="VFR00_04185"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002711579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009027 - tartrate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0046395 - carboxylic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tartrate dehydrogenase"
                     /protein_id="HEU0158484.1"
                     /translation="MAAGAAKSNSMRIAVIPGDGIGNEVLPEGMRVLEAVGRRFDLNF
                     TWDTFDWSCQRFAKTGAMMPADGLAEIARHDAIYLGAVGWPGVPDHVSLWGLLIPIRR
                     NFQQYVNLRPVRLFEGLKSPLADRKPGDIDYLIVRENNEGEYSSIGGRMYEGTELEAA
                     IQQAVFTKKGCERVMRYAFNLAMTRPRRELVSATKSNGISISMPYWDERFAAVGKEYP
                     QVKTSQFHIDILAAHLVRNPTWFDVIVGSNLFGDILSDLGPATTGTIAIAPGGNINPE
                     RAYPSMFEPVHGSAPDIAGKGIANPIGQVWSGAMMLEHLGHTAAAAAIVTAIERLFAE
                     TDVRTRDMGGTASCRELGEALAELVRAR"
     gene            4768..6444
                     /locus_tag="VFR00_04190"
     CDS             4768..6444
                     /locus_tag="VFR00_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU0158485.1"
                     /translation="MRLRPPQGLYLALTLALALAAVASRPAAAQVESVTTLKLREAAA
                     VLQRGNVEQALALYDQVLEDKTLPNDRRAIILNDRGVAYARRQQPKEAIEDFNRAIAL
                     YPEYAALYNNRGNVLLGLGAVREAQRDFDRAVLLAPGYAAAYSNRAGALMRLGEIEPA
                     IADYTKAIALTPSNAAALNGRGRAHLAAQRPHGAIRDFTRAVTADGRFSAAYRSRAEA
                     KFTIKRYEDAIEDFSRAIAFEPANPEVYALRARAYLEADNMASAIKDYAKAIELNPSS
                     AYYAGRGFAYALAEANEEALADLARAIELDPRAANAYAYRAWTYLHQQQTELATKDVA
                     RALKLDANCAVAFWVRGELQEAQGRAELAVTDLRQALSLDPDLNAANRALERLGITER
                     PQQREVAEAGRDGWRVYQKGKQYIARNEEYPRLQIELEMVGKGTPRILEWEVKKPPFQ
                     GIAILRFFAGALDNPRGPEDVEYAAIIDLQANAVVAIEMQRLGGKVATWTWDEGKVVV
                     ANADGITDEFQLRQAKLPVAQAPKRPADPWGWGSERQKKPKSLFDLLFKF"
     gene            6663..7880
                     /locus_tag="VFR00_04195"
     CDS             6663..7880
                     /locus_tag="VFR00_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009735599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEU0158486.1"
                     /translation="MARSFLERGLPGCLSLALLLAGAGCDSPQPSQAKAPAGEQRPAV
                     SVSQPLQRQVVEWDEYTGRFDAVETVEVRARVSGYLSSVHFKDGQPVKPGDLLYDIDP
                     RPFELTLEQARAELLQAQTKVDNANLDVVRGKPLLERRIISEKTFDDRENLVREAQAQ
                     VKVAEAKVKSAELDLSFARVVSPIAGRISRSMVTAGNWVSAGGVSNSTLLTTIVSQDP
                     IHIYFDVSENNYIKYRRLAERGSVAGAADLGATVELALPDERGFPHAGKLDFLDNRLD
                     QGTGTLRARALIANEGGLFSPGMFARVRVTGTPSYAALLLPDEAIGTDQTNKYVYVVA
                     DDGAVARRNIQLGPLVDGLRVVREGLANGDWVITKGLHRARPGQNVAPHRVALTVTAS
                     TPSTAAPPAKVPE"
     gene            7912..11094
                     /locus_tag="VFR00_04200"
     CDS             7912..11094
                     /locus_tag="VFR00_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HEU0158487.1"
                     /translation="MRISRFFIDRPIFAAVLSLVITIIGGIAYFGLGINQLPDITPPT
                     ITVNASYPGASAQTVADTVAAPIEQEVNGVEGMIYMASQSTADGNLSLTVTFDVGTDP
                     DKAQVLVQNRVSAAERKLPDEVRRTGLTVRKRSPDLLLAIHLISPDNTYDQVYISNYG
                     LLNVRDKLMRLYGVGDVSLYGVREYAMRIWLDPERIALRGLTAEQVLDALRAQNVQVA
                     GGALGEPPVDFNNAFQVSLQMKGRLRNANEFEDIIVKIGNDGRLVRLKDIGRVELGAL
                     SYSANGYADRYPAVVIVVDQQPGSNAVRATQGIKDAMAEIAKSFPKGLEYRLTYNPTE
                     FIEVSVAKLYGSIVEATVLVVLVVLLFLKTWRATVIPVVAIPVSLIGTFIVMQGFGFT
                     LNMLTLFGLVLSVGIVVDDAIVVVENFERRLREGLSPVEAARVSMDEVGAAIIAIALV
                     LFAVFVPTTFITGITGQFYRQFAITIASATAISLLVSLTLSPALCALLLKPHSDGAHG
                     KFASVLAPIHAAFRGFDAGFDWMARGYGRLVRGLSRVWLPVLIAYVLLVGFGAWFLQR
                     QPTGFIPTLDRAIVIISLQLPPGASLARTDAVVRRATDLVLSVPGVKFSNAFTGRNAA
                     TFTAATNAALLFLVLDDFEERHKQGQSIDKITREVRAKLGQIEEAQSLVFIPPPVRGM
                     GASAGFSMRLQDTMGLPSNEFAQITQEFVAAANRTPGIANTFTTFQSATPQVFVDVDR
                     DKAQMLKVPISNIFEAMRVFMGSAYVNDFNMFGRTYRVTAQADGDFRLDPESVAKIRV
                     RNADGQMVPLGSLVTFKEIAGPERVPRYNLFPSAEVNGTAQPGISSGQTLEIMKRLTQ
                     EVLPQGITFEWTDLSYQEAKVGRTGYYIFVLSVIFVFLALAAQYESWSLPLAIMLIVP
                     MCLFSAAFGVWLHGREINILTQIGFVVLIALAAKNAILIVEFARQIEERGVDTITAAV
                     EACRLRLRPIIMTSLAFTLGVTPLYWATGAGAEMRIALGTAVFWGMIGVTLFGLIFTP
                     VFYVVIRKLTGRTLVARDKPEPVPLAEAAE"
     gene            complement(11122..11916)
                     /locus_tag="VFR00_04205"
     CDS             complement(11122..11916)
                     /locus_tag="VFR00_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016844097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEU0158488.1"
                     /translation="MSNSPVLLSLDDRGIARVALNRPEVGNAYDGALLAALSAALAAL
                     PDTPPPRALLLQGNGRHFQAGADLNWVRAVAEGSLPENLAASRATAEAIDQLNRLPVP
                     SVALVQGGCFGGGTGLIAACDIVIAAEDAMFSIAEVRWGLTAAIIIPQLSDAISVRQV
                     RRYALTGERFGAAEALRIGLVHKLVARAELAAAGEEMLAKLLQNGPAAMAETKALALE
                     SAWGGFAPARLAALIDAHALKRRSAEAREGLLSFVEKRAARWPPPA"
     gene            12245..13609
                     /locus_tag="VFR00_04210"
     CDS             12245..13609
                     /locus_tag="VFR00_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEU0158489.1"
                     /translation="MTGRFRHWRHTVCLAVGLVFAAAAAPAFAQGGEWRWWDGTPVFA
                     SPAYQTRRASEEDPRRKPEPPPNDLRPNSAPLRSEAVVEALELAIQRYQAIAGRGGWP
                     TIPGSRMIRPEDDDERLPLLRERLMITGELSRRQSSSSSFMFGASYDDLEPAIRRYQE
                     NNGLRVTGRVDKATLQALNVPAVARLAQLRLNLQRIKDLSLQRLEDRYVLVNVPAFQL
                     EAVERHQVELRHRVIAGKPERQTPTVRASIKAINFFPYWRVPDSVASLDLIPRLIKEP
                     DYLQKEQIRVLTGSFNGAELDPNNIDWRQADVSKIKFRQDPGLQNALGLVRLDMPNEH
                     GVYMHDTPMKPLFNQRGRAFSAGCVRVQDVFQLVEWVLKYESGWEQTGRVRDVLDAGQ
                     PLDVSLKQPVPVYFAYITAWAEPDGRVVFRPDIYGRDGVRDLVASHERDPGDGPPPPF
                     TLAP"
     gene            complement(13725..15125)
                     /locus_tag="VFR00_04215"
     CDS             complement(13725..15125)
                     /locus_tag="VFR00_04215"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEU0158490.1"
                     /translation="MQLEQLSARALAAAIHAKKISSLEATAAAIKRLEAAHALTNCTL
                     SLEAEAALAAARACDAAIAAGENVGPLAGVPLAHKDMFDRQGKIASWGARIRAPAPAT
                     EDATVIARYKAAGALQIAALHLTEFAFGPTGHNYVLGHARNPWDPTRITGGSSAGTAA
                     AVAYGAIPAGLGSDTGGSLRLPAAACGVSSIKPTWGRISRAGAMPLSPALDTIGVIAR
                     HVEDLALMLGLLAGPDARDPAASPVPVPDYVARLDDPIRGLRLGIDAAVIGSAHPQVQ
                     EMLQQVVSILGKQGLVSAACRFPDWQTLDHLVQLTQMPDCSAAHAAYLRSRPDDYGPQ
                     VRARLELGHFIGAVDHLTALRARGSTLARTLQETFKDIDIAILPILADPLPTIAELDI
                     GGGPTLQAAMARVVKYTRPVNYLGLPTLALPVPRHGGLPNGIQLIGRPYREAQLLAVG
                     QAYQREVPPEIARPLA"
     gene            complement(15125..15322)
                     /locus_tag="VFR00_04220"
     CDS             complement(15125..15322)
                     /locus_tag="VFR00_04220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0158491.1"
                     /translation="MTQEPQVAEPKSSEASDLSIPPERRRAAQAHVAILAATAAKVAA
                     DLPLGADVDDFRRILMAEARR"
BASE COUNT         2518 a         5214 c         5066 g         2588 t
ORIGIN      
        1 agcttgcaca cttcctgagc gatcgacggc gcaatgtcgt cctcatcggt cccggcgccg
       61 gcgtcggcag agccacggcg accagcgtgc tcggcgtgct cgcctccccg gcggcggtgg
      121 tgctggacgc ggacgccctc acctcctttg cggcgaacgc ggaggcgggc gagctgcgcg
      181 cggtgggttt cggctttgtc gtgcgcgatc cggagcccgc gccggaagca aaggcgctgt
      241 ttgcggcgat caaggcgcgc ccggcgccgg tcgtgctgac cccgcatgcg ggcgaatttg
      301 cccgcctctt tggcgagctg cctggctcga aactcgatta tgcgcgcaag gcagccgagc
      361 tcagcggcgc cgtgctgctg ctcaagggcg ccgataccgt ggtggcggcg cccgacggcc
      421 gggccgccat caacgacaac gccccgcctt ggttggcgac cgccggctcg ggagacgtct
      481 tggcaggctt cgtcggcggt ctgttggggc aaggcatgcc ggccttcgag gctgcctgcg
      541 cggccgtctg gctgcatggg gccgcggcgc aggcgttcgg ccccggcctg atcgccgagg
      601 acctcccgga aaccttgccg caggtgctgg cacggctcag ctttgtcggg acttgatgag
      661 cccggcgcgg cgcgctcagc gaggcgaggc ggccctcgcc cgttgccttg cacgccaacc
      721 cgttcgggtt gtaacaggtg gctccacttc cgcttgtaaa gggaaaacgc ccgttgaacg
      781 atgagcccac agcccgcgcc gcgccggcgc gggctgccgt cgccagcgcc attgaattgc
      841 gcaatctcgc cgttgccttc acgttgccga gcgggtcgag ctaccaggcg gtggccgcca
      901 ccaatctcaa cgtgggcgcg cgcgagttcg tcgccgtcgt ggggccaacg ggctgcggca
      961 aatccaccct gctcaacgtc gccgccggcc tgttgccgcc gagcgccggg gaggccttca
     1021 tcttcggggc gccgctgcta gggctcaacc aatccgcggg ctacctgttg caacaagatg
     1081 cgctgatgcc ctggaagacg gcgcgcgaca acgtcgccat cggtctggag gtcggcggca
     1141 cgccgcgccg cgaggccttg ccggccgccg acggctggct gaagaacgtc gggcttgccg
     1201 catttggaga tcgctatccc caccagctct ccggcggcca aaagaagcgc gtcggccttg
     1261 cgcaggtctt gatccgcgac cccaaaatcc tgctgatgga cgagcccttc ggacccctcg
     1321 atgcgcaaac gcgccagatc atgggcactt tgctgctcga actgtgggcc aaggaccgca
     1381 aggcggttct gttcatcacc cacgacctgg aagaggccat cgcgctggcc gatcgcgtgc
     1441 tgatcatgtc ggcggggccc gaagccggca tcatcggcga ttacagcatc gatctcggcc
     1501 ggccacgcga tgcctccgag atccgcctgc tgccgcagtt tcacgccctg cataaagcca
     1561 tctggagcca gctcaaagac gaggtcatgc gggcctacgc tgggggcggc gcatgaagct
     1621 gtcgcgggcg ggcctcctga gcctgcaggt cctcgtcgcc gtcctcatca ttggcatttg
     1681 gcagctggga tcgaccgtct ccgtcggcgg ctggtatttg tttcccaagt tcttcttttc
     1741 cacgcccggg gaggttgcgc ttcgggtctg gagtttgttt gcggacggca ccgccaagat
     1801 cgtcacctgc ccagagccca tcgacatttc ccgcatccta gtggtctccg atccctgctt
     1861 cggcaagacc atctggcagc atttgctcat caccttgacg gaggggcttc tggcttttgt
     1921 gatcggcgcg gtcagcggcg tcgcgctcgg cttctggctc gcgcgcaatc cattgctgtc
     1981 ggcgatcttt gacccctaca tcaagatgct gaacgcgctg ccccgggtgg tgctggcgcc
     2041 gatcttcatg ctctggctgg ggctcggcat ctggtccaag gtggcgctag gcgtgacgtt
     2101 ggtgttcttc atcgtgttct tcaacgtcta ccagggcgtc aaagaggtca gtcccgtcat
     2161 cctcgccaac gcgcgcatgc tgggcatgaa cccgcgccag ctgttcgtgc acgtctattg
     2221 gccctcggcg ctgtcgtgga tgttctcctc gctgcacacc tcggtgggct ttgccatcat
     2281 cggtgcggtg gtcggcgaat acttgggctc tgccgccggg ctcggctatc tgatccagca
     2341 ggccgaggga accttcgata ccaccggagt atttgcgggg atggtgatcc ttgcggtatt
     2401 cgtgctgatc atcgattggc tcgtcaccat catcgaaaat cgcctgctgg tttggcgacc
     2461 gaccggcagc ggcgcccggg cctgaagcaa tcgctaagcg gagacgaaca tgagctgtcg
     2521 caagttcttg attatcgccg tgctcgcgct gctgggcatg ggccgggccg aggcacaggc
     2581 gcccgagcgc aaggacgtga gattgggcgt cggcggggcg cctgcacttt attacctgcc
     2641 gctcgcgctc accgagcggc tcggctattt caaggagcag gggctcaacg tcgagatcaa
     2701 cgacttcaag ggcggctcgc aatcgctgac cgccctgatc ggcggctccg ccgacgtggt
     2761 caccggtgcc tatgagcaca ccttgcgcat gcaggggaaa ggccaggacg tcgtcgccat
     2821 catcgagctc ggccgctatc ccggcatctc gctcgccgtc aagaaggacc gcgccgacaa
     2881 gatcaaaacc gtggccgacc tcaagggagc caaaatcggc gttaccgcac cgggctcatc
     2941 gaccaatatg atcgtgtggt atttgatggc caaggccggg ctgaagcccg acgatgcgtc
     3001 cttcatcggc gtcggcaccg gcgcgggcgc cgtggccgcc atccaaaggg gcgaaatcga
     3061 tgccatctcc aacatcgatc cggtgatggc caagctcgaa tcgacggggg acatcgttac
     3121 gctggcggag acgcgcacca ccgagggcac ggccaaggtg ttcggtgggc cgatgtcggc
     3181 cgccgtgctc tatgccaaac gcgactggct cgagaagaac cccaacacgg cccaggccct
     3241 caccaacgcc ttctacaaga cgctgaagtg gatgcgagat gccaccccgg aggcgattgc
     3301 cgccacggtg ccgcaggagt attggctggg cgacaaggcg ctctacaccg ccgcggtcaa
     3361 agcaaacatg caagtctact cgcaggacgg cgtcgtcagc gccgagagcc gccagcgctc
     3421 gctcgatttt ctcaagcagt ttgataaaga aatcgccggc gccacgatcg atagcgccaa
     3481 gacctgggat gaccggttcg tgaaaaaggc tgccgccacg gtgcgctagt cgtcgccgcc
     3541 tggcggccgc ggagcttgga cgaggaggaa cgaggatggc ggcaggagca gcaaaatcga
     3601 acagcatgcg cattgccgtt atccctggtg acggcatcgg caacgaggtg ctgccggagg
     3661 gcatgcgggt gctcgaagcg gtcggccggc gcttcgatct caatttcacc tgggacacgt
     3721 tcgattggtc ctgtcagcgt tttgccaaga cgggagccat gatgccggcg gacgggctcg
     3781 ccgagatcgc caggcacgat gccatctacc ttggcgccgt cggctggccg ggcgtgcccg
     3841 atcacgtctc gctgtggggt ctcttgatcc cgatccggcg taattttcag cagtacgtca
     3901 atttgcgccc ggttcgcctg ttcgaaggac tgaagtcgcc gctggccgac cgcaagcccg
     3961 gcgacatcga ttacctgatc gtgcgcgaga acaacgaggg ggagtattcc tccatcggcg
     4021 ggcgcatgta tgagggcacc gagctggagg cggccatcca gcaggccgtc ttcaccaaaa
     4081 aaggctgcga gcgcgtgatg cgctacgcct tcaacctggc gatgacgcgc ccccgccggg
     4141 agctggtatc ggccaccaag tcgaacggca tctccatttc catgccctac tgggacgagc
     4201 gctttgccgc ggtcggcaag gaatacccgc aggtcaagac cagccagttc catatcgaca
     4261 ttctcgccgc gcacctggtg cgcaacccga cctggtttga cgtgatcgtc ggctccaatc
     4321 tgttcggcga catcctgtcg gatctgggcc ctgccaccac gggcaccatt gccattgccc
     4381 cgggcggcaa catcaatccc gagcgcgcct acccctccat gttcgagccg gtgcatggca
     4441 gcgcgccgga catcgccggc aagggcatcg ccaaccccat tggtcaggtc tggtccggcg
     4501 ctatgatgct cgagcacctc ggccacacgg ccgccgccgc ggccattgtc acggcgatcg
     4561 agcgcctgtt tgcggagacc gacgtgcgca cccgcgacat gggcggcacg gcgagctgcc
     4621 gcgaactggg ggaggcgctg gccgagctcg tgcgcgcacg ctgaagtggc ggcgccggcg
     4681 ggcattttcg gcgccagccg attccggtta taaccggggc taagacatgg gggcgcccgc
     4741 aggccacccc cgagcgaagg agcctcgatg cggctgcggc caccccaggg tttatatctt
     4801 gccttgacgc tggcgcttgc gctggcggcc gtggcctcgc gacccgccgc ggcgcaggtc
     4861 gagagcgtca ccaccttgaa gctgcgcgag gccgccgcgg tcctgcagcg cggcaacgtg
     4921 gagcaggcgc tggcccttta cgaccaggtg ctcgaggaca agacgctccc caacgatcgg
     4981 cgcgccatca ttctcaacga tcgtggcgtt gcctacgccc gccggcagca acccaaggag
     5041 gcgattgagg atttcaatcg cgccatcgcg ctctatcccg agtatgccgc gctctataac
     5101 aaccgcggca acgtgctgtt ggggctcggc gcggtgcgcg aggcacagcg ggatttcgac
     5161 cgcgccgtgc tgttggcgcc cgggtacgcc gctgcctaca gcaaccgggc cggagcgctg
     5221 atgcggcttg gcgagatcga gccggcgatt gccgactaca ccaaggcgat cgcgctgacg
     5281 ccgagcaatg ccgccgccct caatgggcgg ggccgcgcgc atttggcggc ccagcgtccg
     5341 catggagcga tcagggattt tacccgcgcg gtcacggcgg atgggcgctt cagcgccgcc
     5401 tatcgcagcc gggcagaggc gaagttcacc atcaagcgct atgaggatgc gatcgaggat
     5461 ttcagccggg ccatcgcctt cgagccggcc aatcccgagg tctatgcgct gcgggcccgc
     5521 gcctacctgg aagccgataa tatggcttcc gccatcaagg actacgccaa ggccatcgag
     5581 ctcaacccca gctcggccta ctacgccggc cggggcttcg cctatgcctt ggcggaggcc
     5641 aacgaggagg ccctggccga cctggcgcgc gccatcgagc tcgatccacg cgcggccaac
     5701 gcctatgcct atcgcgcctg gacctacctg caccagcagc agacggaact ggccacgaaa
     5761 gacgtcgcgc gcgccttgaa gctcgatgcc aactgcgccg tcgccttctg ggttcgcggc
     5821 gagctgcagg aggcccaggg gcgggccgag ctcgccgtta ccgatctgcg ccaggccctc
     5881 agcctcgatc ccgatctcaa cgccgccaac cgtgcgctgg aacggctcgg catcaccgag
     5941 cgcccgcagc agcgcgaggt tgccgaagcg ggccgcgacg gctggcgggt gtaccagaag
     6001 ggcaaacagt acatcgcccg caacgaggag tacccacgcc tgcagatcga gctcgagatg
     6061 gtgggcaagg gcaccccgcg catcctggaa tgggaggtga agaagccgcc gttccaaggc
     6121 atcgccatcc tgcgcttctt tgccggcgcg ctcgacaacc cgcgcgggcc cgaggacgtc
     6181 gagtatgccg ctatcatcga tttgcaggcc aatgccgtgg tcgccatcga gatgcaacgc
     6241 ctcggcggca aggtcgccac ctggacctgg gacgagggca aggtggtcgt cgccaacgcc
     6301 gacggcatta ccgatgagtt ccagttgcgc caggccaagc tgcccgtggc gcaggccccc
     6361 aagcggcccg ccgatccctg gggctggggc tccgagcgcc agaagaagcc caagtcgctg
     6421 ttcgacctcc tgttcaagtt ctagcggcct cgccttgtcg tggcggcggc agcggtcgca
     6481 ggccgccgcc gcggatgggg gtgcggcgcg ctggagcgcc tggcccacag cgggcacggg
     6541 cgcgcctcga gcgctggttc accgccgcat aagatgttaa gggaactttg aggtgggacg
     6601 ggtatcgggc ctcctggggc ttacgatggg gcggcccgcc cctgcttgca gcgagtccaa
     6661 agatggcgag aagctttctc gagcgcggtc tgcccggctg tctgagcctg gcgcttttgc
     6721 tggccggcgc cggctgcgac agtccgcagc cgagccaggc caaggcgccg gccggggaac
     6781 agcgtcccgc cgtttccgtg agccaacccc tgcagcgcca ggtggtggaa tgggacgaat
     6841 acaccggccg cttcgatgcg gtggaaaccg tcgaggtgcg ggcccgcgtc tccggttatc
     6901 tctccagcgt tcatttcaag gatgggcagc cggtcaagcc gggcgatctg ctttacgaca
     6961 tcgatcccag gccattcgaa ttgaccctcg aacaggcgcg cgccgaactg ttgcaggcac
     7021 aaaccaaggt cgacaacgcc aacctcgacg tcgtgcgcgg caagcctttg ctggaacggc
     7081 gcatcatctc ggaaaagacc ttcgacgacc gcgagaacct ggtgcgcgaa gcgcaggcgc
     7141 aggtgaaggt ggccgaagcc aaggtgaaaa gcgccgagct cgacttatcg ttcgcccgcg
     7201 tcgtctcccc catcgccggc cgcatcagcc gcagcatggt cacggccggc aactgggtct
     7261 cagccggcgg cgtcagcaac agcacgctgc tgacgaccat cgtcagccag gaccccattc
     7321 atatttactt cgacgtgagc gagaacaact acatcaagta tcggcgcctg gccgagcgcg
     7381 gcagcgtcgc gggggccgcc gatctcggcg ccaccgtgga gctggcgctg cccgacgagc
     7441 gcggcttccc gcacgcgggc aagctcgact ttctcgacaa tcgtctcgac caaggcacgg
     7501 gcacgctgcg cgcccgggcc ctcattgcca acgagggcgg tctattttcg ccaggcatgt
     7561 tcgcgcgcgt acgcgtcacc ggcacgccca gctacgccgc tctgttgctg cccgacgagg
     7621 cgatcggcac cgaccagacc aacaagtacg tctatgtggt ggccgatgac ggtgcggtgg
     7681 cgcgtcgcaa cattcagctg ggcccgctgg tcgatggcct gcgcgtggtg cgcgagggcc
     7741 tggccaacgg cgactgggtg atcaccaagg gcctgcaccg cgccagaccc ggccagaacg
     7801 ttgcgcccca tcgcgtggcg ctgacggtta ccgcatcgac gccgagcacc gccgcgccgc
     7861 cggccaaggt gccggaatga aaggcggcgc gagggttgga gggctgacgc ggtgagaatc
     7921 agccgcttct ttatcgaccg gccgatcttt gccgcggtgc tttcgctcgt gatcaccatc
     7981 attggcggca ttgcctattt cgggctcggc atcaaccagc tgcccgacat caccccgccg
     8041 actatcaccg tcaacgccag ctatcccggg gccagcgcgc aaaccgtcgc cgacacggtt
     8101 gccgcgccca tcgaacagga ggtcaacggc gtcgagggca tgatctatat ggcctcgcaa
     8161 tcgaccgcgg acggcaacct gtcgctgacc gtcaccttcg acgtcggcac cgacccggac
     8221 aaggcgcagg tgctggtgca aaaccgcgtc agcgccgccg agaggaagct gcccgatgaa
     8281 gtgcgccgca ccgggctgac cgtgcgcaag cgctccccgg acctgctgtt ggcgatccac
     8341 ttgatttcgc ccgacaacac ctacgatcag gtctacatct ccaactacgg tctgctcaac
     8401 gtccgcgaca agctgatgcg gctttacggc gtgggcgacg tctcgctgta cggcgtacgc
     8461 gaatatgcca tgcgcatctg gctcgacccg gaacgcatcg cgctgcgcgg gctgacggcc
     8521 gagcaggtgc tcgatgcctt gcgcgcccag aacgtgcagg tggcgggcgg ggccttgggc
     8581 gagccgcccg ttgacttcaa caacgccttc caggtctccc tgcaaatgaa gggccgcctg
     8641 cgcaatgcca atgaattcga ggacattatt gtcaagatcg gcaacgacgg ccgtctcgtg
     8701 cgcctcaagg acatcggccg ggtcgagctc ggcgcgctca gctactcggc gaacggctat
     8761 gccgaccgct accccgccgt ggtcatcgtc gtcgatcagc agccgggcag caacgccgtg
     8821 cgcgccacgc agggcatcaa ggacgccatg gcggagatcg ccaagagctt ccccaagggt
     8881 cttgaatacc gcctgaccta caatccgacc gagttcatcg aagtgtcggt ggccaagctc
     8941 tacggcagca ttgtcgaggc cacggtgctg gtggtcctcg tcgtcttgct gtttctcaag
     9001 acctggcgcg ccacggtcat ccccgtcgtg gcgatccccg tgtccttgat cggcaccttt
     9061 atcgtcatgc agggcttcgg cttcaccctc aacatgctga ccctgttcgg cctggtgctg
     9121 tcggtgggca tcgtggtcga cgacgccatc gtcgtggtgg aaaacttcga gcgccgccta
     9181 agggaggggc tctcgcccgt ggaggcggcg cgcgtctcga tggacgaggt cggcgcggcg
     9241 atcattgcca tcgcgctcgt gctgtttgcc gtattcgtac ccaccacctt catcaccggc
     9301 atcaccggcc aattctaccg ccagttcgcg atcaccatcg cctcggcgac cgcgatatcg
     9361 cttctcgtct cgctcacctt gtccccggcc ctgtgcgccc tgctgctcaa gccgcattcg
     9421 gatggggcgc atggcaaatt cgcctctgtg ctggcgccaa tacacgccgc gtttcgcggc
     9481 ttcgatgccg gcttcgactg gatggcccgc ggctatgggc ggctggtacg gggcctgtcg
     9541 cgggtctggc tgcccgtact catcgcctac gtcctgctgg ttggtttcgg cgcttggttc
     9601 ttgcagcgcc agccgaccgg cttcatcccc acgctcgatc gcgccattgt cattatctcg
     9661 ctgcagctgc cgccgggcgc ctcgttggcg cgcaccgatg ccgtggtgcg gcgcgccacc
     9721 gacctggtgc tgtccgtgcc cggcgtgaaa ttctcgaatg ccttcacggg ccgcaacgcc
     9781 gccaccttca ccgccgcgac caatgccgcc ctgctcttcc tggtgctcga cgacttcgag
     9841 gaacgccaca agcagggcca gtcgatcgac aagatcaccc gcgaggtgcg cgccaagctg
     9901 ggtcagatcg aggaagcgca gtccttggtg ttcatcccgc cgccggtgcg cggcatgggt
     9961 gcgtcggccg gtttttccat gcgcctgcag gacaccatgg gcctgccctc caacgagttc
    10021 gcgcagatca cccaggaatt cgttgccgcg gccaatcgca cgccggggat tgccaacacc
    10081 ttcaccacct tccagtcggc aaccccgcaa gtgttcgtcg acgtcgaccg cgacaaggcg
    10141 cagatgctca aggtgccgat cagcaacatc tttgaggcca tgcgcgtctt catggggtcg
    10201 gcctacgtca acgacttcaa tatgttcggg cgcacctacc gcgtgacggc ccaggccgac
    10261 ggcgacttcc ggctcgaccc ggagagcgtc gccaagatcc gcgtgcgcaa tgccgacggc
    10321 caaatggtcc cgctcggcag cctcgtcacc ttcaaggaga tcgccgggcc ggagcgcgtg
    10381 ccgcgctaca atctcttccc ctcggcggag gtgaacggga ccgcccagcc cggcatcagc
    10441 tcgggccaga cgctcgagat catgaagcgg ctgacgcagg aggtgctgcc gcagggcatt
    10501 accttcgagt ggaccgacct gtcctatcag gaggccaagg taggccgcac cggctattac
    10561 attttcgtgc tgagcgtgat tttcgtcttc ctggcgctgg ccgcccagta cgagagctgg
    10621 tcgctgccgc tcgccatcat gctgatcgtg ccgatgtgcc tgttctcggc ggctttcggc
    10681 gtctggttgc atgggcgcga aatcaacatt ttgacccaga tcggcttcgt ggtgctgatc
    10741 gcgctggccg ccaagaacgc cattctgatc gtggagttcg cgcgccagat cgaggagcgt
    10801 ggcgtcgata cgattacggc cgcggtggag gcctgccggc tgcgcctgcg gcccatcatc
    10861 atgacctcgc tcgccttcac gctcggcgtc acgccgctct actgggcaac gggcgcgggc
    10921 gcggagatgc gcattgctct gggaacggcg gtgttctggg gcatgatcgg ggtgacgctg
    10981 ttcgggctga tctttacccc ggtgttctac gtggtcattc gcaagctgac cggccggacg
    11041 ctcgtggccc gcgacaagcc cgaacccgtt cccctggccg aagccgccga gtagcgccca
    11101 cggcgaagcc ttggcggccg cctaagccgg tggtggccac cgcgctgcgc gcttttcaac
    11161 gaacgagagc aggccttctc tcgcctccgc cgagcgccgt tttaacgcat gcgcgtcgat
    11221 cagggccgca agcctggcgg gggcaaagcc gccccaggcg ctctccagcg ccaaggcctt
    11281 ggtctcggcc atggcggccg gcccattctg caaaagtttg gccagcatct cctcgccggc
    11341 tgccgcgagc tcggcgcggg cgaccagctt gtgcaccaag ccgattctca gcgcctccgc
    11401 cgccccgaag cgctcccccg tcagggcgta gcggcgcacc tgacgcaccg agatcgcatc
    11461 gctcagctgc ggaatgatga tggcggcggt gagaccccag cgcacctcgg caatggaaaa
    11521 catggcatcc tcggcggcga tgacgatgtc gcaggcggca atcagcccgg tgccgccgcc
    11581 gaaacatccc ccctgcacca gcgccacgct gggcaccggc aagcggttga gctggtcgat
    11641 cgcctcggcg gtggcgcggc tggcggccag gttctccggc aagctgccct cggcaacggc
    11701 gcgcacccaa ttgaggtcgg cacctgcctg aaaatgccgg ccgttgcctt gcagcagcag
    11761 ggcccgcggt ggcggcgtgt cgggaagcgc cgccaacgcc gccgacagcg ccgcaagcaa
    11821 tgccccgtcg taggcgttgc cgacctccgg ccgattgagc gccacccggg caatgccgcg
    11881 gtcatcgagg cttaagagca cgggactgtt cgacatgggt gcggcctccg ctgaattgct
    11941 ggtccagtat ggcggcgcgg catcgccagg gcaaaaatgc cgtttcggca agcattcccg
    12001 ggcgctgccg ggtcgggctt agtcggaatg tcttcctttg gccacatctg tgtgggccta
    12061 accggcgccg aggacgctgg catgcggttg cgcctgccca gcgggggccg caatcaccat
    12121 gttacactgt tgtgaagggc gtgtgaggcc gcgcggcttg ctatcgctgc cgttgcacac
    12181 tagacgcgag atggcggggt gcgtcgcgcc agcgatgcac cagcatgagg gagatcggca
    12241 gggcatgacc ggcaggttca ggcattggcg gcacacggtc tgccttgcag tggggctcgt
    12301 tttcgcggcg gccgcagcac ccgctttcgc gcagggcggc gaatggcgct ggtgggacgg
    12361 aactccggtc tttgcctccc cggcctatca gacgcgccgg gcgagcgagg aggatccgcg
    12421 ccgcaagcct gagccgccgc ccaatgactt gcggccaaac tcggccccgc tgcgcagtga
    12481 ggcggtggtc gaggcgctcg agctcgccat ccaacgctac caggcgatcg cggggcgcgg
    12541 cggctggccg accattcccg gcagccgcat gatccgcccc gaggacgatg acgagcgcct
    12601 gccgctgttg cgcgaacgcc tgatgatcac cggcgagttg tcgcggcgcc agagcagctc
    12661 ctcctccttc atgttcgggg cgagctacga cgacctcgag ccggccattc gccggtacca
    12721 ggaaaacaac ggcctgcgcg tcacgggccg ggtggacaag gccacgctgc aggcgctgaa
    12781 cgtgcccgcc gtcgcccgtc tggcgcagct gcgcctcaac ttgcagcgca tcaaggacct
    12841 ctcgctgcag cggctcgagg atcgctatgt gctcgtcaac gtgccggcct tccagctcga
    12901 ggccgtcgag cggcatcagg tggagctgcg gcatcgcgtg atcgccggca agccggagcg
    12961 gcagaccccc accgtgcgcg cctccatcaa ggccatcaac ttcttcccct actggcgggt
    13021 gcccgacagc gtggcgagcc tcgatctgat cccacggctc atcaaggagc ccgactacct
    13081 gcagaaggaa cagatccggg tgctgaccgg ctccttcaac ggagcggagc tcgaccccaa
    13141 caacatcgat tggcgccagg cggacgtgag caagatcaaa ttccgccaag acccggggct
    13201 gcagaatgcc cttggcctgg tgcgcctcga catgcccaac gagcacggcg tctacatgca
    13261 tgacacgccc atgaagccct tgttcaacca gcggggccgc gccttcagcg cgggttgcgt
    13321 gcgcgtgcag gatgtctttc agctggtcga gtgggtcctg aaatatgaat ccggctggga
    13381 gcagacggga cgcgtgcgcg acgtcctcga cgcgggccag ccgctcgacg tcagcctgaa
    13441 gcagccggtg cccgtctatt ttgcctacat cacggcctgg gcggagccag acggccgcgt
    13501 cgtgttccgg ccggacatct atggccgcga cggcgtacgc gatctcgtcg ccagccacga
    13561 acgcgatccc ggtgacggcc cgccgccgcc cttcacgctg gcaccctagt ccgccaaccg
    13621 ccccggcgcg gaccgcccca aaccgcgacg cacgcgcgca cgcgcgtgcg cacccgagag
    13681 agggtttcgc accgcgcttc cgaccttgcc gggagccagc gccttcaggc gagcgggcgg
    13741 gcaatctcgg gcggcacctc gcgctgatag gcctgtccca cggccaagag ctgtgcctcc
    13801 cgataggggc gcccgatcag ctggatgccg ttgggaaggc cgccgtggcg cggcaccggc
    13861 agggccaagg tgggcaggcc caaataattg accggacgcg tatatttcac cacacgcgcc
    13921 atcgccgcct gcagggttgg cccgccgccg atatcgagtt cggcaatggt cgggagcgga
    13981 tcggccaaga tcggcaggat ggcaatgtcg atgtctttga aggtttcctg cagcgtgcgc
    14041 gccagcgtgc tgccgcgcgc ccgcaaggcc gtcaggtgat cgaccgcgcc gatgaaatgt
    14101 ccgagctcca agcgggcccg cacttgcggg ccataatcgt cggggcgcga gcgcaggtag
    14161 gccgcgtgcg cggccgagca gtccggcatc tgtgtcagct gcaccagatg atcgagggtt
    14221 tgccaatcgg gaaagcggca ggcggccgac accaggccct gcttgcccag aatgcttacc
    14281 acctgctgca gcatctcctg cacctgtggg tgggcgctgc cgatcacggc cgcatcgatg
    14341 ccgagccgca agccccggat cgggtcatcg agccgggcaa catagtcggg caccggcact
    14401 gggctggcgg cggggtcgcg cgcgtcggga ccggccagga ggcccagcat gagcgccaga
    14461 tcctccacgt ggcgcgcgat cacgccgatg gtgtcgagcg ccggggacag cggcatggca
    14521 ccggcgcgac tgatccggcc ccacgtcggc ttgatggagc tgacgccaca ggccgccgcc
    14581 ggcaagcgca gggatccgcc cgtatcggag ccgaggccgg cgggaatggc accataggcc
    14641 accgccgctg ccgtgccggc cgaggagccg ccggtgatcc gggtcggatc ccaggggttg
    14701 cgcgcatgcc ccaacacgta gttgtggccg gtcggaccga aggcgaactc ggtcaaatgc
    14761 aaggcggcga tttgcagggc gccggcggcc ttgtagcggg caatcaccgt cgcatcctca
    14821 gtggccggcg ccggcgcgcg gatgcgcgca ccccagctcg ctattttgcc ctgccggtcg
    14881 aacatgtcct tgtgggccag gggaacgccg gccagcggcc cgacattttc gccggccgcg
    14941 atcgccgcat cgcaagccct agccgccgcc agtgccgcct ccgcttcgag gctcaaggtg
    15001 caattggtga gcgcgtgcgc cgcctccagg cgcttaattg cggccgcggt cgcctccagg
    15061 ctggagatct ttttggcatg gatggccgcg gccagcgccc gcgccgagag ctgctcgagc
    15121 tgcattatcg cctcgcctcg gccatgagaa tgcgacggaa atcgtcaacg tcggcgccga
    15181 gcggcaagtc ggcggcgacc ttggccgccg tggccgccag aatggcgacg tgcgcctgtg
    15241 cggcgcggcg ccgctccggc ggtatgctga ggtccgatgc ctcgctcgac ttcggctcgg
    15301 cgacttgcgg ttcttgcgtc atgcgacctc ctggatcccc ggggacggca agcctagccg
    15361 caggcgcggg cgcccacaac cgacgc
//
LOCUS       DASTMT010000188         3780 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_1738837, whole genome shotgun sequence.
ACCESSION   DASTMT010000188 DASTMT010000000
VERSION     DASTMT010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3780)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3780)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3780
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1738837"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..226)
                     /locus_tag="VFR00_04225"
     CDS             complement(<1..226)
                     /locus_tag="VFR00_04225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0158492.1"
                     /translation="MAKFVIEPHFRLQEWVAQEKGYFGAEGLDYDFREFVRASGGAHH
                     AAPGKVGAFQSIEKGREANVTCACHWTVNVA"
     gene            567..2105
                     /locus_tag="VFR00_04230"
     CDS             567..2105
                     /locus_tag="VFR00_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018648239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0158493.1"
                     /translation="MSRAVRFGVMLTGILMLALPLLGSGAAQAQKRGGSLTVGLELDI
                     AGFDPLKVGVFDTSANMAAALIFDTLTSLDDKGEAQPKLALSWSHADDYKTWTFKLRP
                     GVKFHDGTAFDAQALKFNFDRQKDPKNKCRCAFYIAFLKEVQAPDPLTAIFVLTDPSV
                     NLPKIMAFASSNNVVQSPAAMQGRGDDYNRNPVGTGPFVLKSWTAGDRMVLEKNPSYW
                     NEGRPYLDRVVLRPLPDSQARFASLEAGESDLIWDDEFDSDNILKAKKTASLSVHQHV
                     GSGASVYAFNTKAAPFDDVRVRRALVMALDRPKWSQVLTNGLSRPATNPYGEGSWVKC
                     KDDGALPFDLAKAQALIKEYGKPVKFKMLVTATPRGRANGQVLQQFWKLAGAEMEIEQ
                     VDQTAIVTRAFARQFELTPWRIVDLADPDPQMYANFRSGSPVALANYANPELDQLLER
                     ARVTADPEKRSEDYCAISRLINSEAIWFWGFQNTYYAIAKAKVKGLPKLHNGVIDVSP
                     TWLE"
     gene            2115..3065
                     /locus_tag="VFR00_04235"
     CDS             2115..3065
                     /locus_tag="VFR00_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018322758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0158494.1"
                     /translation="MLGFIATRIGYLVPVLIAVTLLTFLIASLLPGDLAHAMLGDQAT
                     PEAVEALRRDMGLDQPLWWRYLHWLAGIVQGDFGRSFRTGEPVLGAILARLPVSLELM
                     LLAELTALAVSIPLAIHCAAHSGGTFDRVVTGIAFAKLSIPNFMAAILLIYLFAVELR
                     WLPATGYVPFAEDGLGNVRAMVLPALTLALAEWPVLMRVLRSDMIAVLQEDYITMARA
                     KGLKSRRILFVHALKPSSLTLITVAGINIGRLIGGAVIVESVFALPGIGRLLLGAIYT
                     RDFMILQGVVLFVAAGFVIVNFFVDLLYAVLDPRIRHAHR"
     gene            3052..>3780
                     /locus_tag="VFR00_04240"
     CDS             3052..>3780
                     /locus_tag="VFR00_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016846521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0158495.1"
                     /translation="MRTAEAVAGSLVRSATEAWARLAGLGWLFGLALAWIAVIVIAAG
                     LADLLPLPSPTDMDMLARREPPSLAHWLGTDRLGRDMLARLIHGARISLTVAILAPLI
                     GLLIGGALGILAGYFRGGLETVVVGGVDVLLAFPPLVLALAVMAYLGQTLANLTYIIG
                     VLSIPAFTRVARAATLSIAKREFILAARAMGATELRILLRELLPNVILPLAAFFLLAV
                     AVTIVVEGALSFLGLGVPPPAPSWG"
BASE COUNT          583 a         1301 c         1195 g          701 t
ORIGIN      
        1 cagcaacgtt gaccgtccag tggcaggcgc aggtgacgtt ggcttcccgg cccttttcga
       61 tcgactggaa ggcgccgacc ttgcccgggg cggcgtggtg ggcaccgccg ctggccctga
      121 cgaactcgcg aaaatcgtaa tcgagcccct ccgcgccgaa gtagcccttt tcttgcgcca
      181 cccactcctg caggcggaaa tgcggctcga tcacgaactt ggccatgcgg ggccctcccg
      241 gcgcgctgac ccgcgccacg acagtatccc cctgatgtta gcactgccgc gccgttgcag
      301 gcgagagcgc gcgttaatgc tttcgccggc gctcgagccc caacccggcg cgccgcggct
      361 gccagcccca agccacaggc cgcccctttc cgcggcccgc gcgccgatcc cgaattgcct
      421 atcgctgggg gggaccatat tctctgctgc ggcagtcccg ctcaaccaag tcgcgcatgg
      481 ccgagcaagc aagggcgggg acgcggccgc ttcttgagcg ccgcgggttg cgcaggggaa
      541 acgctgacct gaggaggaga acgccgatgt cccgagcggt gcgttttggc gtgatgctga
      601 ccggcatcct aatgctcgct ttgccgcttc tcggcagcgg cgcggcgcag gcccagaagc
      661 gcggcggcag cctcaccgtt gggctcgaac tcgacatcgc cggctttgat cccctgaaag
      721 tcggggtgtt cgacacctcg gccaacatgg cagcggcgct catcttcgat accttgacga
      781 gcctcgacga caagggggag gcgcagccga agctcgcgct ctcctggagc catgccgacg
      841 actacaaaac ctggaccttc aaattgcggc ccggggtcaa attccacgac ggcacggcct
      901 ttgacgcgca ggcgctcaaa ttcaatttcg accgccagaa ggatcccaag aacaagtgcc
      961 gctgtgcctt ctacatcgcc ttcctcaagg aggtgcaggc ccccgatccc ttgaccgcta
     1021 tcttcgtcct gaccgacccg tccgtgaacc tgccgaagat catggccttc gccagctcca
     1081 acaacgtcgt gcagtcaccg gccgccatgc agggacgcgg cgacgactat aaccgcaatc
     1141 ccgtggggac cggacccttt gtcctcaagt cctggaccgc aggcgaccgc atggtcttgg
     1201 agaagaaccc cagctactgg aacgagggcc ggccctacct cgatcgtgtg gtgctccgcc
     1261 cgctgccgga ctcgcaggct cgcttcgcca gcctcgaggc cggcgagagc gatctcatct
     1321 gggatgacga attcgacagc gacaatatcc tgaaagccaa gaagacggcg agcctcagcg
     1381 tgcaccagca cgtgggctcc ggggccagcg tctacgcctt caacaccaag gctgcgccct
     1441 tcgacgacgt gcgcgtgcgc cgggccctgg tgatggcgct cgatcgcccg aagtggagcc
     1501 aggtgttgac caacgggctc agccgtcccg ccaccaaccc ttatggcgag ggctcctggg
     1561 tcaaatgcaa ggacgacggc gctctgccct tcgacttggc caaggcgcag gcgctgatca
     1621 aggagtacgg caagcccgtc aaattcaaga tgcttgtgac ggcgacgccg cgcgggcgcg
     1681 ccaatggcca ggtcttgcag cagttctgga agctcgccgg tgccgagatg gagatcgagc
     1741 aggtcgacca gaccgccatc gtcacccgcg cctttgcccg ccagttcgag ctcacgccct
     1801 ggcgcatcgt cgacctcgcc gatcccgatc cgcagatgta tgccaacttc cggagcggca
     1861 gccccgttgc gctcgccaac tacgccaatc ccgagctcga ccagctgctc gagcgtgccc
     1921 gggtcacggc cgaccccgag aagcgcagcg aagactactg cgccatcagc cggctcatta
     1981 acagtgaggc gatctggttc tggggcttcc agaacaccta ctacgccatc gccaaggcaa
     2041 aagtgaaagg gctacccaag ctgcacaacg gcgtgatcga tgtgtcgccg acctggctcg
     2101 aatagttcac gctcatgctg ggcttcatcg ccacccgcat tggctatctc gtccccgtcc
     2161 tgatcgccgt cacgcttttg accttcctga tcgcctcgct gctgccgggc gatttggcgc
     2221 acgccatgct cggcgatcag gcaacgcccg aggccgtcga ggccctgcgc cgcgacatgg
     2281 ggctcgatca gccgctctgg tggcgctatc tgcattggct cgccggcatc gtgcagggcg
     2341 acttcggccg ctccttccgc acgggcgagc ccgtgctggg cgccattctg gcgcggctgc
     2401 cggtgtcgct cgaattgatg ctgctcgccg aactgaccgc gctcgccgtc tcgatcccgc
     2461 tggcgatcca ctgcgctgcc cacagtggcg gtaccttcga tcgcgtggtg accggcatcg
     2521 ccttcgccaa gctgtcgatc ccgaacttca tggcggcgat cctgttgatt tacctgttcg
     2581 cggtcgagct caggtggcta ccggccacgg gctacgtgcc cttcgcggag gacgggctcg
     2641 gcaatgtgcg ggccatggta ttgccggcgc tgaccttggc gctggcggaa tggccggtgc
     2701 tcatgcgcgt cttgcgctcc gacatgatcg cggtcctgca ggaggactac atcaccatgg
     2761 cgcgcgccaa gggcctgaag tcccgccgta ttcttttcgt gcacgccctg aaaccctcct
     2821 cgctgacgct cattaccgtc gccggcatta atatcggccg gctgatcggc ggtgccgtga
     2881 tcgtggaatc ggtgtttgcc ctgccgggca tcggccggct cctgctcggg gccatctaca
     2941 cccgcgattt catgatcctg cagggcgtcg tgctattcgt ggccgcgggc ttcgtgatcg
     3001 tcaatttctt cgtcgatttg ctctatgccg tcctcgatcc caggatccgt catgcgcacc
     3061 gctgaggccg tcgccggcag cctcgtgcgc agtgccaccg aggcatgggc ccgcctggcg
     3121 ggcctcggct ggctgttcgg gctggcgctg gcctggatcg ccgtcatcgt catcgccgcg
     3181 ggattggccg acttgctgcc actgccgagc cccacggata tggacatgct ggcgcggcgg
     3241 gagccgccca gcctcgcgca ttggctgggc acggatcgct tgggacgcga catgctggcc
     3301 cgcctgatcc atggcgcgcg catttcgctg accgtcgcca tcctcgcgcc gctgatcggg
     3361 ctcctgatcg gcggcgcgct ggggatcctg gcgggctatt ttcgcggcgg gctcgagacg
     3421 gtggtggtgg gcggcgtcga tgttctgctc gcctttccgc cgctagtgct ggcgctggcg
     3481 gtgatggcct acctgggcca gacgctggcc aatctcactt acatcatcgg ggtcttgagt
     3541 attccggcct ttacccgcgt ggcgcgggcg gcgacgttga gcatcgccaa gcgcgaattc
     3601 atcctggcgg cgcgcgccat gggggcgacg gagctcagga tcctcctgcg cgaactgctg
     3661 cccaacgtta tcctgccgct cgccgctttt ttcctgctgg cggtcgccgt taccatcgtg
     3721 gtcgagggcg ccttgagctt tttgggcctg ggggtgccgc cgccggcgcc gagctggggc
//
LOCUS       DASTMT010000189         4949 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_4646088, whole genome shotgun sequence.
ACCESSION   DASTMT010000189 DASTMT010000000
VERSION     DASTMT010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4949)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4949)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4949
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4646088"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            405..695
                     /locus_tag="VFR00_04245"
     CDS             405..695
                     /locus_tag="VFR00_04245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0158496.1"
                     /translation="MTKLLSKVGISAAALALAAFAFTAQAQQKAPDKAAPKAAAQPKC
                     KTIKQQAGCEGRSDCSWVAESKDAKGKVKAKAYCRSKPAPKKEAPKKEAPKK"
     gene            complement(1145..1663)
                     /locus_tag="VFR00_04250"
     CDS             complement(1145..1663)
                     /locus_tag="VFR00_04250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0158497.1"
                     /translation="MLGRILSVFFGFVLACLAAGLTLVLFVYTPAELASLNAGAAVPR
                     LSEAGLLALAAATHAGVFAAPFALLGAVIGEWRRFRGLGYYALVGIGIAGLGFFLQYR
                     SEAAGDLSILNTYALSAFALTGLIGGGLYWLAAGRHAGGRGPLQSTAHGPSDGIASPA
                     ETASAAAELPKA"
     gene            complement(1764..2189)
                     /locus_tag="VFR00_04255"
     CDS             complement(1764..2189)
                     /locus_tag="VFR00_04255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0158498.1"
                     /translation="MLRTVGRLIWVPIAFVLSALVSLFVILSLGQERVMQAMAGRKPD
                     QTTVDAAFELFGVVIQFFSGYTLLPAILLVIVGEVARLRGALYYVVGGGITLAAVPFL
                     TRLHQPVSTLGVAEVVWQVLATAGFAGGFVYWLLAGRKA"
     gene            complement(2208..3416)
                     /gene="rlmN"
                     /locus_tag="VFR00_04260"
     CDS             complement(2208..3416)
                     /gene="rlmN"
                     /locus_tag="VFR00_04260"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020187859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEU0158499.1"
                     /translation="MSDTQHAPPLDVAAATAKPSLAGLDRGRLGAALRAAGVPEAQLK
                     MRTAQLWSWIYVRGATSFAEMSDVAKDLRNALAGAYSLDRPQIVREQISEDGTRKWLL
                     RLARRGHEVRQPEIETVYIPEVDRGTLCISSQIGCTLTCSFCHTGTQRLVRNLEVAEI
                     VGQILLARDRIGDWPARSGEAPQRPETAAPGRGPAERKITNVVLMGMGEPLYNFANVR
                     DAMAIVSDGDGVALSKRRITLSTSGVVPEIARWGEEAGTMLAISLHAVRDDLRDKLVP
                     INRKYPIAQLMAACRAYPGLSNARRITFEYVMLKDVNDSLVDAKALIKLLAGIPAKIN
                     LIPFNPWPGTVHTASDWEQIERFAEIVNRAGYASPVRTPRGRDIMAACGQLRSESLNV
                     RARERPAAVR"
     gene            complement(3513..4817)
                     /gene="hslU"
                     /locus_tag="VFR00_04265"
     CDS             complement(3513..4817)
                     /gene="hslU"
                     /locus_tag="VFR00_04265"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011508728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HEU0158500.1"
                     /translation="MTSFSPREIVSELDRYIVGQNDAKRAVAIALRNRWRRQQLTGEL
                     REEVLPKNILMIGPTGCGKTEISRRLAKLASAPFLKVEATKFTEVGYVGRDVDSIVRD
                     LVGVGIGLVRESKRKEVKALAEKNAEERVLDALVGANASAGTREAWRKRLRNNELNDK
                     EIEIQVAETGPQFPTFDIPGGQVGMLNLNDMLGKAFGQRLKPRRASVGQSYDLLVAQE
                     SDKLLDQDQITQDAVRSVENDGIVFLDEIDKITSRSEAGRVGADVSREGVQRDLLPLL
                     EGTTVSTKYGPVKTDHVLFIASGAFHLAKPSDLLPELQGRLPIRVELKPLTREDFRRI
                     LTEPEASLIKQYVALMATEGVTLDFSADGVDALADIAAQVNSSVENIGARRLQTVMER
                     VLDEISFAAADRTGETIRIDAAYVTARIGDLAKNADLSKFIL"
BASE COUNT          909 a         1583 c         1641 g          816 t
ORIGIN      
        1 gaggtatcga gcccgccgga gtaggcgagc accacctttg tcactttgtc cttgtcgccc
       61 tgggcaggca tcgggggtct cacttttgaa ccgctcctca acgcgggcgc actataggca
      121 acttgccacc ccgggcaagc cagctaggcc ggccccagcg cacggctcgg cctgccatcc
      181 gtccttcgga ctagaggcag gcaattgcgc tttgatctgg cgcctttttc tcaagcccgc
      241 ggcgggcggc agccgcgcgc gtcggtgcgg gttcgcattt cggcgagccc ggccgcgcgc
      301 cgcattcgcc gccgccgcgg cgctgcgccc gcccaaacgt tcatggggta ttcatcagac
      361 gactcacatt aatcggccgg gaccaacgat cggaggctat ccccgtgacc aaactgttga
      421 gcaaagttgg catctcagcc gccgcgcttg cgctggctgc gttcgccttt actgcgcaag
      481 cgcagcagaa ggcaccggac aaggccgcgc cgaaggcggc ggcgcagcca aagtgcaaaa
      541 caatcaaaca gcaggcgggg tgcgagggac gttcggactg cagttgggtg gcggagagca
      601 aggacgccaa gggcaaagtg aaagccaagg cgtactgtcg gtcgaaaccg gctccgaaga
      661 aagaggctcc gaaaaaagag gccccgaaaa agtaaagaca cgcagcagcg caccgggacg
      721 gaaggcagcg cgtctgcgcc cgcacccggt gcttgcgccg tcttgccgcg attgggtcgc
      781 gtcgtggccg ttcaagacgc tcgcccaggt tctccaattc cccgtcgttg gcgacggaca
      841 gcgcaaagac gcgccgatgc cgccggcgcc ttggcccttg gcgttcattg ccgccgctga
      901 tccacggcgc gccgccgtag gcgtcacaaa agcccgtcaa ggcgtcggca gcggccagca
      961 agagtgccga caacagatca gcgataaggc caaggacaaa gccgaggcga gctgcgggtg
     1021 gatcgccggc gtgacaccca gcaagaccgg ccaacacgcg gcgcaggctg ccggaccaac
     1081 tcgggaccgg caaagcaagc aaagagatat gccaagggcg aatacgcccg agcggcggcc
     1141 ggctttaagc cttcggcagc tcggctgccg ccgatgcggt ctcggccggg gaagcgatgc
     1201 catcgctggg cccatgagcg gtgctctgca gcgggccccg gccgccggcg tgacgccccg
     1261 cggccaacca gtagaggcca ccgccgatca gtccggtgag ggcgaaggcg ctgagcgcat
     1321 aagtattgag gatgctgagg tcgccggcgg cttcgctgcg gtactgcagg aaaaagccaa
     1381 ggcccgcgat gccgatgccg acgagtgcgt aataaccgag cccgcggaag cgacgccatt
     1441 cgccgatcac cgcgcccaac agtgcaaacg gggccgcaaa cacgccggcg tgggtcgccg
     1501 cggccaaggc gagcagcccg gcttcactta ggcgaggcac cgccgcgccg gcattgaggc
     1561 tcgcgagctc ggcgggcgtg tagacgaaga gcaccagggt gaggccggcg gcgaggcagg
     1621 ccagcacaaa gccgaagaag acgctaagga tgcggccgag catgaggaac ctccgcgagg
     1681 gtgccgagcc accgagcgct tgagactaaa cgcttcgccc atccatgctc aatcgcgatc
     1741 tctgcctgtg gcgcggcacg gctttaggcc ttacggcccg ccagcagcca gtagacaaag
     1801 ccgccggcga aaccggccgt cgccaggacc tgccaaacga cctcggccac ccccagcgtg
     1861 ctcaccggct ggtgcaggcg tgtcaggaaa ggcactgccg ccagcgttat ccccccgccg
     1921 accacgtaat agagcgcgcc gcgcaggcgg gcgacttcgc ccacgatgac gagcaggatg
     1981 gccggtagca gggtgtagcc ggagaagaac tggatgacga cgccgaaaag ctcaaatgcg
     2041 gcatcgaccg tggtttggtc gggcttgcgc cccgccatcg cctgcatcac ccgctcctgc
     2101 cccagggaga ggatcacgaa caggctgacc agagccgaca gcacgaaggc aatgggcacc
     2161 caaatgagcc gccccacggt cctcagcatg ctttgcgccc catggcctca gcgaacagcg
     2221 gctgggcgct cccgcgcccg cacattgagg ctctcggagc gtaattgccc gcacgccgcc
     2281 ataatgtcac ggccgcgcgg cgtgcgcacc gggctggcat agccggcgcg gttgacgatc
     2341 tcggcaaagc gctcgatctg ctcccaatcg gacgcggtgt ggaccgtacc gggccacgga
     2401 ttgaagggga tcaggttgat cttggcgggg atgccggcga gcagcttgat gagggctttg
     2461 gcgtcgacca ggctgtcgtt gacgtccttc agcatcacgt attcgaaggt gatgcgccga
     2521 gcgttggaaa gtcccgggta ggcgcgacaa gccgccatca gctgcgcgat cggatacttg
     2581 cggttgatgg gtacgagctt atcgcgcaaa tcgtcgcgca cggcgtgcag cgagatcgcc
     2641 aacatggtgc cggcctcttc gccccagcgc gcgatttctg gaaccacacc actggtcgaa
     2701 agtgtgatgc gccgcttcga cagggcgacc ccgtccccgt cggagacaat ggccatggca
     2761 tcgcgcacgt tagcaaaatt atagagcggc tcgcccattc ccatcaggac gacgttggtg
     2821 atcttgcgct cagcggggcc tcggcctggc gctgccgttt cggggcgctg cggagcctcg
     2881 ccgctccggg cgggccaatc gccgatgcgg tcgcgcgcga gcaggatttg gccgacgatc
     2941 tcggccacct cgagattgcg caccaggcgc tgggtgccgg tgtgacagaa cgagcacgtg
     3001 agcgtgcagc cgatctggct cgagatgcac aaggtgcccc gatcgacctc ggggatatag
     3061 acggtctcga tctcgggttg gcgaacttcg tggccccgtc gggcaagccg aagcaaccac
     3121 ttgcgcgtgc cgtcctcgga gatttgctcg cgcacgatct gcggccggtc gagcgaatag
     3181 gcacccgcaa gggcgttgcg caggtccttg gcgacgtctg acatctcggc aaaggaggtc
     3241 gcgccccgca cgtaaatcca gctccagagc tgtgccgtgc gcatcttcag ctgcgcctcg
     3301 ggaacgccgg cggcgcggag cgccgcgccc aggcggccgc gatcaagccc ggcaagggag
     3361 ggcttggcgg tcgctgccgc aacatcgaga ggtggggcgt gctgcgtgtc ggacatgggt
     3421 gcaaggcttg gggaatgacg ccttctatgt agttgcgccg tgctcagctg tcatcagggc
     3481 tggtcagctc gaaaacttat gcgaccaacg cctcacagaa tgaacttgct caaatcggcg
     3541 tttttggcga ggtcgccgat acgggcggtg acgtaggccg catcgatgcg gatggtctca
     3601 ccggtgcggt cggcggcggc gaaggagatc tcatcgagca cgcgttccat cacggtctgc
     3661 aggcggcgcg caccgatgtt ctccacgctc gaattgacct gggcggcgat gtcggccaag
     3721 gcgtcgaccc catcggcgct gaaatcgagc gttacaccct cggtcgccat cagcgccacg
     3781 tattgcttga tgaggctcgc ctccggttcc gtcaaaatgc ggcggaaatc ttcgcgcgtc
     3841 agcggtttga gctccacccg gatcggtagt cgtccctgca gttcgggcaa caggtcagac
     3901 ggcttggcca ggtgaaaagc gcccgatgca atgaacaaga catggtcggt ctttaccgga
     3961 ccgtacttgg tagaaaccgt ggtgccttcc aacaggggta agaggtcgcg ttgcacgcct
     4021 tcgcgcgaga cgtcggcacc gacgcgacct gcttccgagc gcgatgtgat cttgtcgatc
     4081 tcgtcaagga agacgatgcc gtcgttctcg acgctacgca cggcgtcctg ggtgatctga
     4141 tcctgatcga gcagcttgtc gctttcctgg gccactagca gatcgtagct ttggccgacg
     4201 ctggcgcggc gaggcttcag gcgttggccg aaggccttgc cgagcatgtc gttgagattg
     4261 agcatgccaa cctgtccgcc cgggatgtcg aaggtgggaa attgcgggcc cgtttcggcc
     4321 acctgaattt cgatttcctt gtcattgagc tcgttgttgc gcaggcgctt gcgccaggcc
     4381 tcgcgcgtgc ccgccgaggc attggcgccg accaaggcgt cgagcacgcg ctcctcggca
     4441 ttcttttcgg cgagcgcttt cacttccttg cgcttgcttt cgcgcaccag gccgatgccg
     4501 acccctacca ggtcgcgcac gatcgaatcg acgtcgcgac cgacgtaacc gacttccgtg
     4561 aatttggtcg cctccacctt caggaacggt gcactcgcga gcttggcgag gcggcgcgaa
     4621 atctccgtct tgccgcagcc cgtgggtccg atcatcaaaa tgttcttggg cagcacttcc
     4681 tcgcgcagct cgcccgtcag ctgctggcga cgccagcggt tcctgagggc tatggcaacg
     4741 gcgcgcttgg catcgttttg gccgacgatg tagcgatcga gctcggacac gatttcacgg
     4801 ggcgaaaagc tggtcatgcg gcgctcgtcg gctggctgtc acgaatcatc aagagggcgc
     4861 gaccgtcgca ggtatccacc tcccggtcga tggcaaaccc ggccttctga taggccctga
     4921 tggcgcgcgc gttggcgggg ctcggatcg
//
LOCUS       DASTMT010000190         9929 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_1738964, whole genome shotgun sequence.
ACCESSION   DASTMT010000190 DASTMT010000000
VERSION     DASTMT010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 9929)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9929)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9929
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1738964"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..706)
                     /locus_tag="VFR00_04270"
     CDS             complement(<1..706)
                     /locus_tag="VFR00_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020180887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEU0158501.1"
                     /translation="MSLDVVDLREFYGRPLGSAVRRLLTRRIRARWRNVQGAQLMGLG
                     FAVPYIGMFRGEAASLGALMPATQGAVAWPAGDSVQTVLVEEALLPLADASLDRLLAV
                     HCLEATERSGPLLREIWRVLKPEGRLLLVVPNRRGVWTRLDITPFGHGRPYSRGQLER
                     LLHDALFTPLEWTGALFMPPIDRPFILRWSTVFERLGARMWPGFAGVMIVEARKELMG
                     AIPAGAKSARRGGLATA"
     gene            825..1595
                     /gene="gloB"
                     /locus_tag="VFR00_04275"
     CDS             825..1595
                     /gene="gloB"
                     /locus_tag="VFR00_04275"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013949677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HEU0158502.1"
                     /translation="MPKLEIEQFPCLSDNFGVLIHDAAANLTASIDAPDAAAVTRALA
                     AKGWRLSHILTTHHHADHTGGNLALKAETGCKIIGPRGEKGKVPGIDEAVGQGDTVRF
                     GAFGLRVLDTPGHTAGHITYWCPEANVAFVGDTLFAIGCGRVIEGTAEMMWDSLQKLM
                     ALPKETAVYCGHEYTQANARFALTIEPDNAALKARAKEVDQLRTAGKPTLPTRIGLEL
                     ETNPFLRPEVAAIQQRLGMVGRPAWQIFAEIRERKNRS"
     gene            1601..2050
                     /locus_tag="VFR00_04280"
     CDS             1601..2050
                     /locus_tag="VFR00_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020083971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEU0158503.1"
                     /translation="MSANEALSAEEVVALLGLAPHPEHGHYRETFRDAATVGEGRAAS
                     TAIYFLLREGERSRWHRVDAAEVWHWYAGAPLLLRIAAARERASLRLGSDLAAGERPQ
                     AVVPAGAWQSAESCGAWTLVGCTVAPGFEFAGFELAPDDFDPPAAAA"
     gene            2389..4143
                     /locus_tag="VFR00_04285"
     CDS             2389..4143
                     /locus_tag="VFR00_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEU0158504.1"
                     /translation="MKRTAGGACLSAVFVDYDNIYLSLKRKSEEAAKRFARDAHVWLR
                     EIESGRLITGTHDSFSETRRRIVMNRCYGNPVPRRNAHDNSTDMNSFPFVRHHFLRAG
                     FEVVDCPPLTAQLKNSSDIRMVMDVRDYLTHETYFDEFIILSGDADFTPVLHRLRAHA
                     RRTVIYANDHTAAPYTAISDGEVRESDLIALILNGEVPADDDAEAASGPATPAAKIEA
                     ARRAIVAEIVADVRGSPAPMLLEALADRTVRALGHEKTIATGWAGAGSFRELLRQGLP
                     PDISMTEEPPYLVVDLKRHGQAVAAPKAREPARELAVAPAQSAPRFAASAAADAAPLI
                     ATTGSAAAPAPARPASPQPPASGPAAAPSAAGVQQSIARIQEACQAPAMAPHEYRLLF
                     ELMAEEINENDLIGAQTLANITHRAQERGLEVRRDDVRFILEVVSESDPWFEQGASAN
                     LFAGRFRNFVVARCRGQGLNLSADELDLVDAWFAGGNMPPAPQAQATPAARAAAARPA
                     ATQSAAAGRTGAVPVSLPTPAPAPAAAAAGGRWWSSETGAVEPPNKEAAPAAPASASG
                     EPAPSEEFPRFVRNRLRG"
     gene            complement(4189..4914)
                     /gene="phbB"
                     /locus_tag="VFR00_04290"
     CDS             complement(4189..4914)
                     /gene="phbB"
                     /locus_tag="VFR00_04290"
                     /EC_number="1.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015670118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018454 - acetoacetyl-CoA reductase
                     activity [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetyl-CoA reductase"
                     /protein_id="HEU0158505.1"
                     /translation="MGRVAVVTGGTRGIGQAISTALKHKGFRVAANYAGNDSAARQFQ
                     ADSGIPIYKWDVADYAACQTGVNRITHDLGPIDVLVNNAGITRDGVLHRMSKENWDAV
                     LRTNLDSVFNMTRLVIEGMRTRGFGRIINISSINGRKGQAGQANYSAAKAGLLGFSKA
                     VALEGAARGITVNVIAPGYIGTEMVQAVPKEVLESKILPQIPLGRLGNVEEIARCVTF
                     LADDAAGFITGACLDANGGQYMA"
     gene            complement(5019..6206)
                     /locus_tag="VFR00_04295"
     CDS             complement(5019..6206)
                     /locus_tag="VFR00_04295"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HEU0158506.1"
                     /translation="MSTDQSTVVIASATRTPVGAFNGSLASLPAHELGKVAIRGALDR
                     ARLEPGEVSEVIMGQILAAGAGQNPARQASILAGIPVDAPAWGMNQLCGSGLRAVALG
                     AQQIQLGSSKIVVAGGQESMSQAPHCAHMREGTKMGDVKFIDTMIKDGLWDAFNGYHM
                     GTTAENVARQWQITREEQDRFAVASQNKAEAARKAGKFKDEITPVTIKTRKGETVFAD
                     DEYIKDGVTYDSIAKLRPAFDKEGTVTAANASGINDGAAAVVLMTLAEAKRRGLTPLA
                     RIVSWAHAGVDPSIMGTGPIPASKKALERAGWTVKDLDLIEANEAFAAQACAVNKGLG
                     WDTEKVNVNGGAIAIGHPIGASGARVLNTLLFEMQRRNAKKGLATLCIGGGMGVAMCV
                     ERD"
     gene            6456..7061
                     /gene="phaR"
                     /locus_tag="VFR00_04300"
     CDS             6456..7061
                     /gene="phaR"
                     /locus_tag="VFR00_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate synthesis repressor PhaR"
                     /protein_id="HEU0158507.1"
                     /translation="MLNKSDPPSDKKEPVVIKKYANRRLYNTETSTYVTLDDLAAMVK
                     SERDFVVFDAKTGDDLTHSVLTQIIVEQESRMGTQTLLPIPFLRQLIRFYGDSIERLV
                     PSYLQISLDTLTKEQERFRKTFAGAFTPAGAFEAYQEQARKNLAMFEQAMAMFSPFKT
                     PPATAPAKAEGEAGKTDELAELKTQLAEMQAKVDKLSRERG"
     gene            complement(7087..7500)
                     /locus_tag="VFR00_04305"
     CDS             complement(7087..7500)
                     /locus_tag="VFR00_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0158508.1"
                     /translation="MSLRALPMIAIPLILYNILVMFSSGDADGFFHAPLLTITMIKGA
                     QWSFSRGDLIILVTMLCLFVELVKSTYTSTASLVDHGLSMLVFIASLVEFLIVNASAT
                     SPFFFILVGALIDVIAGFTIGIRVARRDLSIGADA"
     gene            complement(7616..7801)
                     /gene="rpmF"
                     /locus_tag="VFR00_04310"
     CDS             complement(7616..7801)
                     /gene="rpmF"
                     /locus_tag="VFR00_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009467340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEU0158509.1"
                     /translation="MAVPRKKVSRMKRGTRRSADALRAPTYIEDKDSGERRRPHHIDL
                     KSGKYKGRQVLTPKSEL"
     gene            complement(7938..8945)
                     /gene="mtgA"
                     /locus_tag="VFR00_04315"
     CDS             complement(7938..8945)
                     /gene="mtgA"
                     /locus_tag="VFR00_04315"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012454351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HEU0158510.1"
                     /translation="MAPVPGPRAELRSRPILPAREPAEPSHRAPTGALAPPVEVVREN
                     GAAAERLIRPLPVPVFPRRHAGEPAPSPVQAASLPVWQETPAAHPAPRHAAWTPAAGG
                     DLKLRPILVRALKLGCLGALGCVLLLATLIVLYRWVDPPRSMLMLGQRLGGMPITQHW
                     LSLEHISSNLQLAVIASEDGRFCRHHGVDWEELESAIDQSVDGIARGGSTIAMQTVKN
                     LFLWPAKSYVRKAIEIPLAYAVDATWSKRRLLEIYLNIAEWGPGIFGAEAAARYHFRK
                     PASLLSPREAALLAVSLPNPFERRAGAPGPGTQRLAANLMLRMRAAPGMARCVRSGTG
                     L"
BASE COUNT         1686 a         3306 c         3409 g         1528 t
ORIGIN      
        1 gcgccgtggc gagcccgccg cgacgcgcgg atttggcgcc agccgggatc gcccccatca
       61 gctctttgcg cgcttcgacg atcatgacgc cggcgaaacc tggccacatg cgcgcgccaa
      121 gccgctcgaa gaccgtcgac cagcgcagga taaagggccg gtcgatcggc ggcataaaca
      181 gagcgcccgt ccattccagc ggcgtgaaca aggcgtcgtg caggagccgc tcgagttggc
      241 cgcggctata ggggcggcca tgcccgaagg gggtgatatc gagccgcgtc cagacgccgc
      301 gccggttggg gacgaccagc aacagccggc cctccggctt gagcacgcgc cagatctcgc
      361 gcagcagggg tccggaccgt tccgtcgcct cgagacaatg caccgcgagc agccgatcga
      421 gcgaggcatc tgccaagggc agcaaggcct cctcgaccag cacggtctgc acgctgtcgc
      481 cggcgggcca ggccacggcg ccctgggtcg ccggcatcaa cgccccgagt gaggcggcct
      541 cgccgcggaa catgccgatg tagggcacgg caaagcccag gcccatgagc tgggcgccct
      601 gcacgttgcg ccagcgcgcg cggatgcggc gcgtgagcag gcgacgcacc gcgctgccaa
      661 gcgggcgccc gtagaattca cgtagatcga ctacatccag cgacatcagc gatcagccgc
      721 accactgctg cccgagcttg tttgcgtgcc ccctgttgaa ttcgatgcgc cgcctggtag
      781 ggtgcgggtt ggtggaaaaa aagcaagcga gagcagcgca caccatgccc aagctcgaga
      841 ttgaacaatt tccctgcctc agcgacaatt tcggcgtgtt gattcacgac gcggcggcca
      901 atctgacggc ctccatcgac gctcccgacg cggccgcggt cacccgcgcg ctggcggcaa
      961 agggctggcg tctgagccat attctgacga cgcatcatca tgccgatcat accggaggaa
     1021 acctcgcgct caaggccgag acgggctgca agattattgg gccgcgcggc gaaaagggca
     1081 aagtgccggg gatcgatgag gccgtcgggc agggtgacac ggtgcgcttc ggtgccttcg
     1141 ggctccgtgt cctcgacacc cctgggcata cggcgggcca catcacctac tggtgtcctg
     1201 aggccaacgt cgcctttgta ggggacacct tgtttgccat tggctgcggt cgcgtcatcg
     1261 aaggcaccgc cgaaatgatg tgggactcgt tgcagaagct gatggcgctt ccgaaggaga
     1321 cggccgttta ctgcggacac gaatacacgc aggccaatgc gcgcttcgcc ctgacgatcg
     1381 aacccgacaa tgccgcgctc aaagcccggg caaaagaggt cgatcagctg cgcacggcgg
     1441 gcaagccgac gttaccgacc cgcatcggcc tcgagctcga gaccaacccc ttcctgcgcc
     1501 cggaggtggc ggccatccaa cagcggctcg ggatggtggg caggccggcc tggcagattt
     1561 tcgccgaaat cagagagcgc aaaaaccgca gctaacaccc atgtcggcca acgaggcgct
     1621 gtcggccgaa gaggtggtgg cgttgctcgg cctggcgccc catcccgagc acggtcacta
     1681 ccgcgagacc ttccgggacg cggcaacggt gggcgaggga cgcgccgcct cgacggccat
     1741 ctacttcctg ttgcgagagg gcgagcgctc gcgctggcac cgcgtcgatg cggccgaggt
     1801 gtggcactgg tacgccggtg ccccgctgct gttgcgaatt gcggcggcgc gcgagcgtgc
     1861 gagcctgcgg ttgggctctg acctggcggc cggcgaacga ccgcaagccg tggtgccggc
     1921 cggggcctgg cagagcgccg aaagctgcgg cgcctggacg ctggtcggct gcacggtggc
     1981 gcccggcttc gagtttgccg gttttgagct ggcgccggac gatttcgacc ccccggctgc
     2041 cgccgcatag aattggccgg cggcggcccc gggtggtcgc gccggcccga ggcgcgcggg
     2101 acggcatcca cggccgccac gagcctttgt cgccgtcatg aaaaagcctt gtggggcaaa
     2161 ggtttgtgac ggggacggcg cgggccgggg cggtgtttcg agaggcgcta gccacacgcc
     2221 cagcgggtgg gctttgggcg ccgcggagag cccaaaaatg cagctgggga cgcggcccgc
     2281 ctgcgcttgc ggggtcggag cgactcatat gaagtcccgc gaatcggcaa cctgcccgcg
     2341 aatggggttg gtgcagcaaa aaagacgggg cagtgggggt cagcacagat gaagaggaca
     2401 gctggtgggg cctgtctcag cgcggtgttc gtcgattacg acaacatcta tttgtcgctg
     2461 aagcgcaaaa gcgaggaggc cgccaagcgc ttcgcgcgcg acgcgcacgt ttggctgcgc
     2521 gaaatcgaga gcggccgcct catcaccggc acccatgaca gcttcagcga gacacgccga
     2581 cggattgtca tgaaccgctg ctacggcaat ccggtgccgc gccgcaatgc ccacgacaac
     2641 tccaccgaca tgaattcctt tccttttgtg cgccaccatt ttttgcgcgc tggcttcgag
     2701 gtggtggatt gcccgccact gaccgcgcaa ctgaagaact cctcggatat tcggatggtc
     2761 atggacgtgc gggactacct cacgcacgaa acctacttcg atgagttcat tatcctgtcg
     2821 ggcgatgccg acttcacccc cgtgctgcat cgcctgcgcg cgcatgcgcg ccgcaccgtc
     2881 atttacgcca atgatcatac ggcggcgccc tatacggcga tcagcgacgg ggaggtgcgc
     2941 gaatctgacc tgatcgccct tatcctcaac ggcgaagtgc ctgccgatga tgacgcggag
     3001 gcggccagcg gccccgcgac gcccgccgcc aagatcgagg cagcgcggcg cgcgattgtg
     3061 gccgagatcg tcgccgacgt gcgcgggtcc cccgcaccga tgttgctcga ggcgctcgct
     3121 gaccgcacgg tgcgcgcgct cgggcacgag aaaaccatcg ccaccggctg ggccggcgcc
     3181 ggctccttcc gggagctctt gcgtcagggt ctgccgcccg acatcagcat gaccgaggaa
     3241 ccgccgtacc tggtggtgga tctgaagcgc cacgggcagg cggtggcggc gcccaaggcc
     3301 cgcgagccgg cacgggaatt ggcggtcgcg ccggcgcagt ccgcgccccg ctttgccgcc
     3361 agcgccgccg ccgacgcagc accccttatc gccaccacag gctcggccgc cgccccggcc
     3421 ccagcgcgcc cggcaagccc gcagcccccc gcctccggcc cggcggccgc cccgtccgcg
     3481 gcgggagtgc agcaatcgat cgcccgcatc caggaggcct gccaggcgcc ggccatggcg
     3541 ccgcacgagt accggctcct gtttgagctc atggccgagg agatcaacga aaacgacctg
     3601 atcggcgcgc agacgttggc caacatcacg catcgggcgc aggagcgcgg gctggaagtg
     3661 cgccgtgatg atgtgcgctt cattttggaa gtggtcagcg agtccgatcc ttggttcgag
     3721 cagggcgcct cggccaatct gttcgccgga cgcttccgca atttcgtggt cgcgcgctgc
     3781 cgcggccagg gcctcaacct ctcggccgac gagctcgatc tcgtcgatgc gtggttcgcc
     3841 ggcggcaaca tgccgcctgc accgcaagcc caggccaccc cggccgcgcg cgccgccgcg
     3901 gcccggcctg cggccaccca atcggccgca gccgggcgca ccggtgccgt gcccgtgagc
     3961 ttacccacgc ccgccccggc gcccgccgcg gccgcggcgg gggggcgctg gtggtcgagc
     4021 gagacggggg cggtggagcc ccccaacaag gaagcagccc cagcggcgcc ggcctccgcc
     4081 agcggtgagc ccgcgccgag cgaggagttt ccgcgcttcg ttcgcaaccg gctgcggggc
     4141 tagcacggcg ctggctaacg cgcaggccgc cgcaggcacc aagcgacctc aggccatgta
     4201 ttgcccgccg ttggcatcga ggcaggcgcc ggtgataaag ccggcggcgt cgtcggcaag
     4261 aaaggtgacg cagcgggcaa tctcttccac gttgccgagg cgtccgagcg ggatctgcgg
     4321 caatatcttg ctctccagca cctccttcgg aacggcctgc accatttccg tgccgatata
     4381 gccgggggcg atgacgttga cggtgatgcc gcgcgcggcg ccctcgagcg caaccgcctt
     4441 gctgaacccg agcagccccg ccttcgccgc cgaatagttg gcctgcccag cctgtccctt
     4501 gcggccgtta atcgaggaga tgttgataat gcggccgaag ccccgcgtgc gcatgccttc
     4561 gatcaccaac cgggtcatgt tgaacacgga atcgaggttg gtgcgcagca cggcatccca
     4621 attctccttg ctcatgcgat gcagcacgcc gtcgcgggta atgccggcat tgttgacgag
     4681 cacgtcgata gggcccaggt cgtgggtgat gcggtttacc ccggtctggc aggcggcgta
     4741 atcggcaacg tcccatttgt agatggggat gccgctgtcc gcctggaatt gccgcgcggc
     4801 gctatcgttg ccggcgtagt tggcggcgac ccgaaacccc ttgtgcttga gcgcggtcga
     4861 aatcgcctgc ccgattccgc gcgtcccgcc cgtgaccact gccacccgac ccatgcgcgt
     4921 tttctcccat cagcctgttg ccggccgaga ggataaaaga aaacctcggc cgaatgaaat
     4981 ccggccgagg agaaagttaa gagcgcaaaa ggcttacctc agtcgcgttc gacgcacata
     5041 gcaacgccca tgccgccgcc gatgcacaac gtcgccagtc ccttttttgc gttgcggcgc
     5101 tgcatctcga acaggagggt attgagaacg cgtgccccag aagcgccgat cggatggcca
     5161 atggcgatcg caccgccgtt gacattgacc ttctcggtat cccagccgag ccccttgttc
     5221 accgcgcagg cctgggcagc gaaggcctcg ttggcctcga tcaggtcgag atccttaacc
     5281 gtccaaccgg ccctttccag cgccttcttc gaggcgggaa tcggccccgt ccccatgatg
     5341 gaggggtcaa cgcccgcgtg cgcccaagag acgatgcggg cgagcggcgt caacccgcgc
     5401 cgctttgctt ccgccaaggt catgagcacg acggcggccg cgccatcgtt gatgccgctg
     5461 gcgttggcgg cggttaccgt gccctccttg tcgaaggccg gacgcagctt ggcgatcgaa
     5521 tcgtaggtga caccatcctt gatgtattcg tcatcggcga acaccgtctc gcccttgcgg
     5581 gtcttgatgg tgacgggggt gatttcatcc ttgaatttgc cggccttacg cgcggcctcg
     5641 gccttgttct gcgaggcgac ggcgaagcga tcctgctcct cgcgcgtgat ctgccactgg
     5701 cgcgccacgt tctcggcagt ggtgcccatg tgatagccat tgaaggcgtc ccagaggccg
     5761 tccttgatca tggtgtcgat gaacttgaca tcacccatct tcgtgccctc gcgcatgtgc
     5821 gcgcagtgtg gggcctggct catgctctct tgaccacctg cgaccacgat cttggaactg
     5881 ccaagctgga tttgctgcgc gccgagcgcc accgcgcgca ggcccgagcc gcacagctgg
     5941 ttcatgcccc aggcgggcgc atcgacggga atgccggcca ggatggaggc ctgtcgggcc
     6001 gggttctgcc ctgcgccggc ggccagaatt tggcccatga tgacttccga gacctcgccg
     6061 ggctccagcc gcgcgcggtc gagcgcaccc cggatcgcca ctttgcccaa ttcgtgagcc
     6121 ggcaggctcg cgagcgagcc gttgaaggcc ccgaccggcg ttctggtggc gctggcgatg
     6181 acgacggtgg attggtcagt gctcatccta tgctccaaag cgtgagggtg ttgtgaacag
     6241 ctgggctctt gggcgtcggc ctcgtgctca acgtggtgaa cttgggctgg ggctcacagc
     6301 cacgatagca ggtctcactt cgcacctgca ataagggccg aaaccggcgt ggccggtgct
     6361 acaaccgcgc acaacggact ggtttgcggt gcaccgccgt ggtagcttac gcactgcaaa
     6421 atggccagcc cgtcaagcca taaaaagcga agcccatgct caataaatcg gatcccccca
     6481 gcgacaagaa agagccggtc gttatcaaaa aatacgccaa tcggcggctc tacaacaccg
     6541 agaccagcac ctatgtgacg ctcgatgacc tggctgcgat ggttaaatcc gagcgcgatt
     6601 tcgtggtttt cgacgccaaa acgggggatg acctgacgca ttcggtactg acgcagatca
     6661 tcgtcgagca ggaaagccgc atgggcacgc aaaccctgct gcccattccc tttctgcgcc
     6721 agctgatccg cttttacgga gacagcatcg agcgcctggt accctcttat ttgcagatca
     6781 gcctcgacac cttgacgaag gagcaggagc gctttcgcaa gaccttcgcc ggcgccttca
     6841 cccccgccgg cgccttcgag gcctatcagg agcaagcgcg caagaacctc gccatgttcg
     6901 agcaggcgat ggcgatgttt tctccgttca agacgccgcc tgcgacagcc cccgccaagg
     6961 ccgaagggga ggccggcaag accgacgagc ttgccgagct caaaacccaa ttggccgaga
     7021 tgcaggccaa agtcgacaaa ctttcgcgcg agcgcggcta gccgcgcgcc tcagggcctg
     7081 cccagctcag gcgtccgcgc cgatcgagag atcgcgccgc gccacgcgta tgccgatggt
     7141 aaagccggca atgacatcga tcagcgcacc gaccagaatg aaaaagaagg gcgatgttgc
     7201 cgaggcgttg acgatcagga attcgaccag gctggcgata aaaaccagca tggaaaggcc
     7261 gtgatcaacc agcgatgccg tcgaggtata cgtcgatttc accaactcga cgaacaggca
     7321 aagcatggtg acgaggataa tgagatcgcc gcgcgagaac gaccactgcg cccccttgat
     7381 catcgtaatg gtgagaagcg gggcgtgaaa gaaaccatcc gcatccccgc tcgagaacat
     7441 cacaaggatg ttgtagagga tgagcgggat ggcgatcatc ggcagagctc tgagcgacat
     7501 caaggggctc ccatgcggcg gcggtcagga ttggcaccgc cgggatatca ttgatcatgc
     7561 cggaaggggc ggcaattggg caaagctgac gctttgccag cgacgcgtgc cctcttcaaa
     7621 gctccgactt gggcgtaagt acctgacggc ctttgtattt gcctgacttc agatcaatgt
     7681 ggtgggggcg acggcgctcg ccggaatcct tgtcctcgat gtaggtcggc gcccgcagcg
     7741 catcagctga gcgacgggtg ccacgtttca tgcgtgagac tttcttgcgc gggacggcca
     7801 tgcttcttct ccaaaccaaa gcttaccacc gacgcacggt ggcacgagcg cagtggcggc
     7861 cgaccggtgg agcggatgga tttgccgggc ttatagccgc attcccggcg ggaggccagc
     7921 accaagacga aaccgcctca aaggccggtc cccgagcgca cgcagcgggc catgccgggc
     7981 gcggcccgca tgcgcagcat caaattggcc gccagccgct gggtccctgg gccgggagcg
     8041 ccggccctgc gctcgaaggg gttggggagc gacacggcca gcagcgccgc ctcgcgcggg
     8101 ctgagcaggg aggcgggttt gcggaaatga taacgtgccg ccgcttccgc gccaaaaata
     8161 ccgggccccc actcggcaat gttcaaataa atctcgagta ggcgacgttt ggaccaggtt
     8221 gcgtccacgg cgtaagcaag aggaatctcg atggccttgc gcacatagct cttggccggc
     8281 cagaggaata gattcttcac ggtctgcatg gcgatcgtgc tgccgccccg cgcgatgccg
     8341 tcgacggact gatcgatggc gctttccagc tcctcccaat cgacaccatg gtggcgacag
     8401 aaccgtccat cctcggaggc gatcacggcc agctgcagat tggaggagat gtgctcgagc
     8461 gaaagccaat gctgcgtgat gggcatgccg cccaagcgct ggcccagcat cagcatcgag
     8521 cgtggcggat cgacccagcg atagagcacg atcagcgtcg ccaacagcag gacgcagccg
     8581 agggcgccga ggcagcccag cttgagggcg cgcaccagga tggggcgcag cttcagatcg
     8641 ccgcccgcgg ccggcgtcca ggcggcgtgg cgcggcgccg ggtgcgctgc cggcgtctcc
     8701 tgccagacgg gtaggctcgc ggcctggacc ggggacggcg cgggctcgcc cgcatgccgg
     8761 cgtggaaaga cgggcaccgg caggggccgg atcaggcgct cggccgccgc cccgttctcg
     8821 cggaccacct cgaccggcgg cgccagggcg cctgtcggcg cgcgatggct gggctcggcg
     8881 ggttcgcgcg ccggcaaaat gggccgcgat cgcagctcgg cgcgcgggcc ggggactggc
     8941 gccatcggcg ccgcaacggg cggcggcgct tcaggagcgc cgagggcgcg ctcgggggcc
     9001 cgcgccgacg ccggctcggg cgacgagcgg ggcgcggcgg cagggcgagc cagcgtcttt
     9061 ggcgcgggca cggtcgggtc gcgcgctaac ggcggcgccg cgaccaccgg tgcaggcgcg
     9121 accaccagcg ccggcggccc gggggcaagc ggcgcgctcg ccaattcggc aggcacccgg
     9181 gccagcggcg cgctggcgat tggcgccgcc gtcggccagg tcgggttccc atgccgtaag
     9241 cgtcgcggcc gaggctcggc ggcatcgtgg ctgggcttga gggtgggcgg aacgtccccg
     9301 ctcggagctt gcggcacgcc aggctcacgc tcggcggacg gcatggcacg gtcagcggcg
     9361 gccccgccac gcgcggcggg gggcgtgagg gacggcgagg gcgttgccgg gtgcgacagg
     9421 tctgcgccgc ggagcgctgc agccggggct ccccgcccag gtccggtggg ggctacgccg
     9481 tgcggcccgg ggggcggcaa ggccaagcgc ggcggcggcg ggcttagcgc cagcgtggtt
     9541 ggaagccgcg gcagccaggg cgctggggca ggtcttgccg gcgtccccac cgtggcttgc
     9601 cgcgcttgcc gcgcggccgg cctcgccccc gggagccggc ctggcgccac gggcgaatat
     9661 tcggctgggg ccggcgaggc cgcgggtggg cccgacagag cgccgtcagc cggcgcgccg
     9721 gctgggtctg cgacggccgc ctcgcgcgct ggcgggaggg cccgttcgct cgctggcgcg
     9781 ggaatggccg gggcaaaggc gggcgcatgc ggcggggccg ggctcaggct tggtgccgcc
     9841 gccgggctcc gcggcgcggg cgcggtgccg gcagcctcgg gggcgggcgg caccggtttg
     9901 ccagcgtctg ccgcctcgct cacggctca
//
LOCUS       DASTMT010000191         9034 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_3054434, whole genome shotgun sequence.
ACCESSION   DASTMT010000191 DASTMT010000000
VERSION     DASTMT010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 9034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9034
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3054434"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            36..1130
                     /locus_tag="VFR00_04320"
     CDS             36..1130
                     /locus_tag="VFR00_04320"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HEU0158511.1"
                     /translation="MQIDRVRAHIVQLAADEPLADGKTVHGTTRDLVTVKIRSQEGLD
                     GIGVTFFGGAMTAALARAVEDLGAHAVGEDPHNIERIVEKLRLAAGSSGPGGIFTLAL
                     SAIDTALWDMRGKALGLPLAQLLGGFRARVPAYASGALMRTLSLEEVTTAARRLVERG
                     WSAMKTQLALPGHTSPDIEVERIRLIREIVGSSTQLMCDINQRWLVHEAISIGKRLEP
                     YRLHWLEDVTTADDYAGLARVTQALATPIAGGEYVYGISPFRHMLEARSVDIVMIDLV
                     RVGGITQWMKVAGMAEAFNLPVVSHLIPEIHLHLIAAVRNGEIVEYMPWTRLLFEDPP
                     IPEKGMLTVPAAPGLGLTFRRDISDALDRR"
     gene            complement(1267..1521)
                     /locus_tag="VFR00_04325"
     CDS             complement(1267..1521)
                     /locus_tag="VFR00_04325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0158512.1"
                     /translation="MEQASRSRRGRLPAWGSIILLAGVLLGGCAGSQPLSTGFAGPAQ
                     AGDAERDPGKKTLASKVLTAIALERVTGRKPDPSRLNELN"
     gene            1943..2218
                     /locus_tag="VFR00_04330"
     CDS             1943..2218
                     /locus_tag="VFR00_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0158513.1"
                     /translation="MMARFDVTPFDLTEMATAGGTADALLELGLLSCAGREDVGIDLV
                     SAHKWFNLAALRGNAEAKRYRMEIAHEMSKRQIAEAQRQAREWLARH"
     gene            complement(2236..2772)
                     /locus_tag="VFR00_04335"
     CDS             complement(2236..2772)
                     /locus_tag="VFR00_04335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0158514.1"
                     /translation="MRSNIHIGIAPAARPLVAAGLGVWAGLGGGGIALAQEYCIACTQ
                     PNAVYRCVIEDARPGGQSLTRLCLTALTREGGHANCSVKGGTVFDCNGPVKRVPWAAQ
                     EPGRVAEPAAPEPPANQAKPPAKGEPKTVVDLAKQANENMAKANEEMKEQAQSAGQAI
                     GKATKKTWECMLSLFIRC"
     gene            3059..4465
                     /gene="leuC"
                     /locus_tag="VFR00_04340"
     CDS             3059..4465
                     /gene="leuC"
                     /locus_tag="VFR00_04340"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016847650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HEU0158515.1"
                     /translation="MHPRTQLAKIWDAHVVADLGDNAYLLHVDRHLLHDLGGSRGLLD
                     LKERGLPVHSPHLTFATPDHAISSAPGRSGTSSVGRELLLALREETGAAGIRLFDVDE
                     PGQGIVHVMGPELGLSLPGCLIVCGDSHTCTHGGLGALAFGVGSSELAHVLATQTIVQ
                     RRPKAMRVNFEGSMPIGVTPKDLILRLIGVIGAAGGTGYAVEYAGSAVRALDLEGRLT
                     ICNLSIELGAKMGFMAPDDTTFAYLAGRPFAPKGQMWERAVAHWRQLPSDPGAQFDRE
                     LTLDVASIAPQVTWGTSPEHVIAVDQSIPDPATITDPSRRGGIEAALDYMGLKPNQPI
                     LGTKVDWVFIGSCTNSRLSDLRAAAAVVRGRKVAEGVRAWVVPGSEQIKREAVAEGLD
                     RVFTGAGFQWREPGCSMCLAANGETVPRGERSISTSNRNFVGRQGPGARTHLASPAMA
                     AAAAIAGVITDVRRMGIA"
     gene            4465..5085
                     /gene="leuD"
                     /locus_tag="VFR00_04345"
     CDS             4465..5085
                     /gene="leuD"
                     /locus_tag="VFR00_04345"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016847649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEU0158516.1"
                     /translation="MEKFTQLTAVAAPILRENIDTDIVIPISRMVGNEARGSLGQWCF
                     SALRYRPDGSENPEFILNRAPYRGAQILITGVNFGCGSSREAAVWALKEMGVRCLIGS
                     SFGDIFFNNCFQNGVLPVVLDRATVESLAREVEASQGAGHISVDLAELTLTAPSGKRY
                     AFTVEPQRRQALLEGLDEIAITLQRAPAIAAFQAEDRQRRPWVYEL"
     gene            complement(5122..6147)
                     /locus_tag="VFR00_04350"
     CDS             complement(5122..6147)
                     /locus_tag="VFR00_04350"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEU0158517.1"
                     /translation="MQLGIYLNSQHPEGDDPAQRLAETLEQVRLIRSLGFDSIWAGEH
                     HVTPGFHFFPQLPLLTRVAAEAEGLWLGTNVTLLPLHNPVELAEIAAFLDVASGGRFL
                     LGVGLGYRQEEYAIYGIPMRERVARFTEAIAIIKRLWSEDSVSHHGRYWQFKDASIRP
                     RPLQAPHPPIIVGAQVEAAIARAAKIGDGWLIVPIPTTDQLARQMATYRAARAEANLP
                     ASPHICRLLEVGCASDEDEVLRRVAPFLIEKYKAYFSWGLEGLSLDPNASPEQQFRSL
                     ARNRFAVGTPAEVIEALLAQARAGVRHLSMRVSWPGMPQQDILAGIELLGRAVLPEVR
                     RRTREAA"
     gene            complement(6178..7596)
                     /locus_tag="VFR00_04355"
     CDS             complement(6178..7596)
                     /locus_tag="VFR00_04355"
                     /inference="COORDINATES: protein motif:HMM:NF004767.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HEU0158518.1"
                     /translation="MDRSARNYPLEDIDRETMFHPNTSIVDHLKKGPHIMAEASGVRV
                     RDRQGRELIDCGAGLWCVNIGYGRREMGEAAKRAFENLAYHHIFGGTSNEPIIRLAER
                     VLGLFHEKAGAPHLTKVFFGSGGSDANDTNFKLVRYYNNLRGKPKKKKIISRAGAYHG
                     LTYAAGSLTGIPVYHKAFDLPHPDVLHTSCPHYFRYAAAGESEEAFGGRMVAEVEALI
                     AREGAETIAAFIAEPIMGTGGVLLPPQGYFQKLQALFERHDILFIADEVITGFGRTGH
                     WFATGGMRLKPDIVTLAKGITSAYFPVSASVISEKIWTVLKQASPEFGPVMHGFTYSG
                     HPVGGALGLVNIDIMEREGLIEQAAEVGAYLQELLRARVGAHPHIGDVRGMGQMLAVE
                     FVADKTARRFFAPKAAAHRLVSAKALEFGILTRALPFIEVNSFSPPLNMTRSDAEDAV
                     ERYGKALDAVTPELARLAEQTA"
     gene            complement(7933..9033)
                     /locus_tag="VFR00_04360"
     CDS             complement(7933..9033)
                     /locus_tag="VFR00_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007007322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEU0158519.1"
                     /translation="MLAELIDWVSIESPTYDAVAVNKMESFIAPRIGKLGFNVERLAG
                     RDGLGDILRCRRAAPQDGAGLLLIAHADTVHPVGTLNGKLAIRREADKCFGPAIYDMK
                     GGTVIALAALQAVIDAGQLKLPVTIIINPDEEIGSPTSRELIEAEAKRHKYVLVPEPA
                     KGPRGEVTTGRHAFQRFFLTTHGRPAHSGWTNKDGRSAVRVMAQLVEELESKSNFEQG
                     PTYAVSVFHGGQWVNCVPMTCTAQALCVAQDMATFAEIGRVMDGLAGTRADVRIEIER
                     GPVRPLWTAHAPTLALYARAQRIAGEIGFELPHGQHGGGSDGNFTGALGIATLDGLGV
                     MGAGAHTLEEHILVSSLVPRATLLARLMAELD"
BASE COUNT         1492 a         3025 c         3017 g         1500 t
ORIGIN      
        1 gggccgcgcc agcagggcaa ccggggagag atgccatgca gatcgatcgc gttcgcgctc
       61 acatcgtgca acttgccgcg gacgagccgc tggccgatgg caaaacggtg cacggcacca
      121 cccgtgatct cgtcaccgtc aagatccgca gccaagaggg gctcgatggc atcggcgtca
      181 cattctttgg cggcgccatg acggctgccc tggcacgcgc cgtcgaagac ctaggcgcgc
      241 acgccgtggg cgaggatccc cataacatcg agcgcatcgt ggaaaagctg cgcttggccg
      301 ccggcagcag cggtcccggc gggatcttca cgctggcgct ctccgccatc gacacggcgc
      361 tgtgggacat gcgcggcaag gccttgggct tgccgctggc acagctgttg ggcggctttc
      421 gcgcgcgtgt ccccgcttac gccagcggcg ccttgatgcg cacgctctcc ctcgaggagg
      481 tcacgaccgc agcacgacgc ctggtcgagc gcggctggag cgctatgaag acgcagctgg
      541 cgttgcccgg gcacacctcg ccggatatcg aggtcgaacg aattcggctg atccgcgaga
      601 tcgtgggcag ctccacgcaa ctgatgtgcg acatcaacca gcgctggctg gtgcacgaag
      661 ccatcagcat cggtaagcgc ctggagccct atcggctgca ttggctggag gacgtgacca
      721 ccgcggatga ctacgccggg ctcgcccgcg tgacccaagc gctcgccaca ccgatcgccg
      781 gcggcgagta cgtctatggc atctctccgt tccgccacat gctggaagca cgttcggtcg
      841 atatcgtcat gatcgacctc gtgcgcgtgg gtggcattac ccagtggatg aaggtcgccg
      901 ggatggccga ggccttcaat ctcccagtcg tctcgcacct gatcccggaa attcacctgc
      961 acctcattgc tgccgttcgc aatggtgaga tcgtcgagta tatgccgtgg acccggctcc
     1021 tcttcgagga tccgccgatc ccagagaagg gcatgctgac ggtgccggcc gccccgggcc
     1081 tgggcctcac ctttcgacgc gatattagcg acgcgctcga ccggcgttaa cgccaaggcc
     1141 tgacgcatcg gccgcgggct cggcgcctag tccacgctgc ccgccggcaa aggcgccgag
     1201 gatgggcccg tgcgaggtca cgcagacttc ggcgcgacaa gcagggtcaa ggatcactcg
     1261 aacgcttcag ttgagctcgt tgagccgcga gggatccggt ttgcggcccg tgacgcgctc
     1321 gagcgcaatg gcggtcagca ccttcgaggc cagcgttttt ttgccgggat cgcgctcggc
     1381 atcgccggcc tgggccggcc cggcaaaacc cgtcgatagg ggctgactgc ccgcgcaacc
     1441 gccgagcagc acacctgcca gcaggatgat gctcccccac gccggtaaac ggccgcgtct
     1501 gcttctgctt gcctgttcca tgacgaacac agcagctccc cccaaagtcg acgtgcgcgc
     1561 agcttagccg cgcaaagaca catggcaagc gcaaaatgcc cttgacaaac cgttgctgtg
     1621 gagaggcggg aggggggaac gacagacggg gccggcacgg ccatttttcg cggttgggtt
     1681 agcgccgcgt agcgctaccg atgaacctcc ccttaagcct cgtcccgcgg tgggaggatc
     1741 gtgccgaatc agcgcgcgcc ggtaaggaag tgtttgcgac cggccagcgt gtaacgcgcg
     1801 cattttgtgg cgacatttac gaaatttcat cgccccgttg gcatcttcgc ggcgccacgg
     1861 cggcctctgc cggcgctcgc tcgcgcgcct gacgccgcgc ggcgtgcggc cggtgaccgg
     1921 cggggacaat ataaggacgc gcatgatggc tcgatttgac gtaacaccct tcgacctgac
     1981 cgagatggcg acggccggtg gcaccgcgga tgccctgttg gaactcgggc tcctctcttg
     2041 cgcggggcgc gaagatgttg gcatcgatct cgtcagtgcc cacaaatggt tcaacctggc
     2101 cgcgttgcgc ggcaacgcag aggccaagcg ctatcgcatg gagatcgcgc acgagatgtc
     2161 gaagcgccag atcgcggagg cgcagcggca ggcgcgggag tggctggccc ggcactgagg
     2221 cgcgctcggc ggcgctcagc agcggatgaa cagcgacagc atgcactccc aggtcttctt
     2281 ggtggctttg ccgatcgcct gacccgcaga ctgggcctgc tccttcatct cctcgttggc
     2341 cttggccatg ttttcgttgg cctgtttggc gagatcgacg acggtcttcg gctcgccttt
     2401 ggcgggcggc ttagcctgat ttgccggcgg ctctggcgcc gccggctcgg cgacacggcc
     2461 cggctcctgg gcggcccatg gcacccgctt caccggacca ttgcagtcaa acaccgtccc
     2521 acccttgacg ctgcagttgg cgtgcccgcc ctcgcgggtc agcgctgtca ggcagagccg
     2581 ggttagggat tggccgccgg ggcgtgcatc ctcgattacg caacggtaga cggcattggg
     2641 ctgcgtgcag gcaatgcaat actcctgcgc cagggcaata ccgccgccgc cgaggcccgc
     2701 ccagaccccg aggccggccg cgacgagcgg ccgagctgct ggcgcaatcc caatgtgtat
     2761 gttgctcctc acctcgacct agggcatctc tcgaaaatgc ggcatttcct ggatcttggc
     2821 cgcgcgtgcg ccgtgttttc gcggctgccc cccatcagag cgcctcgagc ttcccatggc
     2881 aaggcggctg aggcgtgtac ggggcgcctt ttctcgccaa cccttgccgc cgtcgcgagc
     2941 cgcccgcaag gttggcccga gcgcgagctt gtcaccatcc cacggcctgc cgcaatatct
     3001 gggccgcagg cctccccctt gagacttgcg ccagccgcaa gaggagccac accccctgat
     3061 gcacccccgc acccagttgg caaaaatttg ggacgcccac gtggtggccg acctcggcga
     3121 taacgcctat ctcttgcacg tcgatcgcca tctcttgcat gacctcggcg gctcgcgcgg
     3181 attgctcgac ctcaaagagc gtggcctccc cgtgcacagt ccgcatctga ccttcgccac
     3241 gccagatcac gccatttcga gcgcgcccgg tcgcagcggc acctcgagcg tcggcaggga
     3301 gctcttgctg gctctgcgcg aggagacagg cgcggccggt attcggctct tcgatgtcga
     3361 cgagcctggg cagggcatcg tccacgtgat gggacccgag ctggggttga gcctgccggg
     3421 ttgcctgatc gtgtgcggcg acagccacac ctgcacgcac ggcggcctcg gcgcgctggc
     3481 cttcggcgtc ggctcctcgg aactggcgca cgtgctggcg acacagacga tcgtgcagcg
     3541 gcggccaaag gccatgcgcg tcaatttcga gggcagcatg ccgatcggcg tcacgcccaa
     3601 ggatttgatc ctgcgcttga tcggcgtgat cggcgcggcc ggcggcaccg gctacgccgt
     3661 cgagtacgcc ggcagcgccg tgcgcgccct cgatctcgag ggccggctga ccatctgcaa
     3721 tctttcgatc gagctcgggg ccaagatggg cttcatggca cccgatgaca cgaccttcgc
     3781 ctaccttgcc gggcgtccgt tcgcgcccaa ggggcagatg tgggagcgcg ccgtcgcgca
     3841 ctggcgccag ctgccgagcg acccgggagc ccaattcgac cgcgagctga ccctcgacgt
     3901 cgccagcatc gcgccccagg tgacgtgggg cacaagtccc gagcacgtga ttgcggtgga
     3961 tcagtcgatc cccgatcccg caaccatcac cgatcccagc cggcgcggcg gcatcgaagc
     4021 cgcactcgac tacatgggcc tgaaaccgaa ccaaccgatc ctgggaacca aggtcgactg
     4081 ggtgttcatc ggctcgtgca ccaacagccg cctgtcggat ctgcgggccg ccgccgccgt
     4141 ggtgagaggc cgcaaggtgg cggagggggt gcgggcctgg gtggtgccag ggtcggagca
     4201 aatcaaacgt gaggcggtgg cggaaggcct cgaccgggtc ttcaccgggg cgggattcca
     4261 atggcgcgag ccaggctgct ccatgtgcct ggccgccaac ggcgagaccg tgccccgcgg
     4321 cgagcgctcg atctcgacct ccaatcgcaa cttcgtcggc cgccaggggc ccggtgcgcg
     4381 gacgcatttg gccagccccg ccatggccgc ggcagcggcc atcgcgggtg tcatcaccga
     4441 cgtgcggcgc atggggatcg cataatggaa aagttcaccc aactgacggc ggtggcggcg
     4501 ccgatcctgc gtgaaaacat cgataccgat atcgtcattc cgatctcgcg catggttggc
     4561 aatgaggcgc gcggcagcct gggacaatgg tgcttcagcg cgttgcgcta ccgaccggat
     4621 ggctcggaaa atcccgagtt cattctcaac cgtgctccct accggggcgc acaaatcctc
     4681 atcaccgggg tgaatttcgg ctgcgggtca tcgcgcgagg cggcggtatg ggcactcaag
     4741 gagatgggag tgcggtgcct gatcggttcg agcttcggcg acatcttttt caacaattgc
     4801 ttccaaaacg gcgtgctccc ggtggtgctc gatcgcgcca cggtcgagag cttggcgcgc
     4861 gaggtggagg cgagccaagg tgccggccac atctcggtcg acctggccga gctgacgctg
     4921 accgcaccat cgggcaaacg ctacgccttc acggtcgagc cgcagcggcg ccaggcgctg
     4981 ctcgaggggc tggacgaaat cgccatcacg ctgcaacgcg cgcccgcgat cgccgcattt
     5041 caggcggaag accgccagcg ccggccctgg gtctatgaac tctgaggcgg gcgccgcccg
     5101 tcgggcgagg cgggcagggc gctaggcagc ctcgcgcgtg cgtcggcgca cctcgggcaa
     5161 caccgcgcgg cccaacagtt cgatgccggc gagaatgtcc tgctgcggca tgccgggcca
     5221 gctcacccgc atgctgagat ggcgcactcc cgcgcgcgcc tgggcgagca gagcttcgat
     5281 cacctcggcc ggtgtgccga cggcgaagcg gttgcgcgcc aggctgcgga actgctgctc
     5341 gggcgaggca ttcgggtcga ggctcaggcc ctccagcccc cacgagaagt aggccttgta
     5401 cttctcgatc aggaaaggcg cgacgcggcg caggacctca tcctcgtcgc tggcgcagcc
     5461 gacctccagc aggcggcaga tatgcgggct tgccggcagg ttggcctcgg cgcgggcagc
     5521 ccgataggtc gccatctgcc gcgccaactg atcggtcgtc gggatcggca cgatcagcca
     5581 gccatcgccg atcttcgccg cgcgcgcgat cgccgcctcg acctgagcgc ccacgatgat
     5641 gggcggatgc ggtgcctgca gcgggcgtgg ccggatgctg gcgtccttga attgccagta
     5701 gcggccgtga tggctgacgc tgtcctcgct ccacagccgt ttgatgatgg cgatcgcttc
     5761 cgtaaaacgc gccacgcgct cgcgcatggg aatgccgtag atggcatact cctcctgccg
     5821 gtagcccaag ccgacgccaa gcaggaaacg cccaccgctg gccacgtcga gaaaggctgc
     5881 gatttcggct agctcgaccg gattgtgcaa cggcaagagg gtgacattgg tgccgagcca
     5941 aagcccctcc gcctcggccg cgacgcgcgt gagcaacggc aactggggaa agaagtggaa
     6001 gccgggcgtg acgtggtgct cgccggccca aatcgaatcg aaccccagcg acctgatcag
     6061 gcgcacctgc tccaacgtct cggcaagacg ttgcgcggga tcgtcgcctt ccggatgctg
     6121 cgaattgaga tagatgccga gctgcatcga tcctttctcc tcctgccgct gcggccgcta
     6181 ggcggtctgc tcggccaacc ttgccagctc cggtgtgacg gcatcgagcg ccttgccata
     6241 acgctcaacg gcatcctccg catcgctgcg cgtcatgttg agcggtggcg agaacgagtt
     6301 gacctcgatg aagggcagcg cccgcgtcaa gatgccgaat tccagcgcct tggcggaaac
     6361 gaggcggtgc gccgcggcct tgggcgcaaa aaagcgccgc gcggttttgt cggcaacgaa
     6421 ctcgacggcc agcatttgcc ccattccccg cacgtcgccg atgtggggat gcgcgccgac
     6481 gcgcgcacgc aggagctctt gcaaataggc gccaacctcg gcggcctgct cgatcaggcc
     6541 ttcccgctcc atgatgtcga tgttgacgag gcccagtgcc ccaccgacgg gatggcccga
     6601 ataggtaaag ccgtgcatga ccggcccgaa ttcgggcgaa gcctgcttca gcacggtcca
     6661 gatcttctcg gagatcaccg aggcggacac cggaaaatag gccgaggtga tgcccttcgc
     6721 cagggtgacg atgtcgggct tcaggcgcat gccgcccgtg gcgaaccagt gccccgtacg
     6781 tccgaagccc gtgatgacct cgtcggcaat gaacaagatg tcgtggcgct cgaacagcgc
     6841 ctgcagcttt tggaagtagc cttggggcgg gagcaggacg ccgcccgtgc ccatgatcgg
     6901 ctcggcgatg aaggccgcga tcgtctcggc gccttccctg gcgatcaagg cttcgacctc
     6961 cgccaccatg cgccccccga aggcttcctc gctctcgccc gcggcggcgt agcggaaata
     7021 gtgcgggcaa gacgtatgca gcacatccgg atggggcaaa tcgaacgcct tgtgatagac
     7081 ggggatgccg gtgaggctgc cggcggcgta ggtcaggccg tggtaggcac cggcgcgcga
     7141 gatgatcttc ttcttcttgg gcttgccgcg cagattgttg tagtagcgga ccaacttgaa
     7201 attggtgtcg ttggcatccg acccgcccga gccaaagaac accttggtaa ggtgcggcgc
     7261 gccggccttt tcatgaaaca ggcccaagac gcgttcggcg aggcgaatga tcggctcgtt
     7321 cgaggttccc ccgaagatgt gatgataggc gagattttcg aaggcccgct tggcggcctc
     7381 gcccatctcg cgccggccat agccgatgtt gacgcaccac aggccagcgc cacagtcgat
     7441 cagctcccgg ccctgccggt cgcggacgcg cacgccgctg gcctcggcca tgatgtgcgg
     7501 gcccttcttg aggtgatcga cgattgaggt gttcggatgg aacatcgttt cccggtcgat
     7561 gtcctcaagg ggatagttgc gggctgatcg gtccattgtt cacctctgtg gcggcttccc
     7621 gagcgaacgc tgcggcatgc aaactcggcg gcagcatacg cgcgcgctgg gcaacgtgcc
     7681 accggcgaaa gcggactgcc ttgccgcggg ccctgtgcgc gcgagccagg gcgcggcatc
     7741 gccgcccggc cctggcggcg ggaaggcgca agcggcggga caaccggcgc gcgaagccca
     7801 tacggccacc gcgaccgggc gggcacgcat tgccgatgct gccgctgctc ggccccgacg
     7861 gcaacgacgg ttggcaggcc ggcgcgcgcc tctcctcgcg ctcaaggccg aggcgctcgg
     7921 caggtcccgg tctcagtcga gctccgccat cagccgagcg agcagggtgg cgcggggtac
     7981 gagggacgac acgaggatgt gctcctccag cgtgtgggcg cccgccccca tcacgcccag
     8041 gccgtccaag gtcgcaatgc cgagcgcgcc cgtgaaattg ccgtccgagc cgccgccgtg
     8101 ctggccgtgc ggtagctcga agccgatctc gccggcaatg cgctgggcgc gcgcgtaaag
     8161 cgccagggtt ggcgcgtggg cggtccacag cgggcgcacg ggtccgcgtt caatttcgat
     8221 acgtacatcc gcgcgggtgc cggccaggcc atccatgacg cggccgattt cggcaaaggt
     8281 cgccatgtcc tgggcgacgc acagggcctg cgcggtgcag gtcatcggca cgcagttcac
     8341 ccactgccca ccgtggaaca ccgagaccgc gtaggtcggg ccttgctcga agtttgactt
     8401 gctctcgagc tcctccacca gctgcgccat cacgcgcacc gccgaacgcc cgtccttgtt
     8461 ggtccaaccg gaatgagccg gacgcccgtg cgtagtgagg aagaagcgct ggaacgcgtg
     8521 ccggccggtc gtcacttcgc cgcgcgggcc cttggcgggc tccgggacca aaacgtactt
     8581 gtgccgtttc gcctcggctt cgatcagctc gcgcgaggtc gggctgccga tctcctcgtc
     8641 gggattgatg atgatggtga ccggcagctt gagctggccc gcatcgatca ccgcctgcaa
     8701 cgccgccagc gcgataacgg tgccaccctt catgtcgtag atggcgggac cgaagcactt
     8761 gtctgcctcc cgccggatcg ccagcttgcc gttgagggtg ccgacgggat gcacggtatc
     8821 ggcgtgggca atcagcaata ggcccgcccc gtcctgcggc gcggcgcggc ggcagcgcag
     8881 gatgtcgccg agcccgtcgc ggcccgccag ccgctcgacg ttaaagccga gcttgccgat
     8941 cctgggcgcg atgaagctct ccatcttgtt gacggcgacg gcatcatagg tggggctttc
     9001 gatgctgacc caatcgatga gctcggccag cagg
//
LOCUS       DASTMT010000192         1697 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_430920, whole genome shotgun sequence.
ACCESSION   DASTMT010000192 DASTMT010000000
VERSION     DASTMT010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 1697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1697)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1697
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_430920"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..556)
                     /gene="obgE"
                     /locus_tag="VFR00_04365"
                     /gene_synonym="cgtA"
                     /gene_synonym="obg"
                     /gene_synonym="yhbZ"
     CDS             complement(<1..556)
                     /gene="obgE"
                     /locus_tag="VFR00_04365"
                     /gene_synonym="cgtA"
                     /gene_synonym="obg"
                     /gene_synonym="yhbZ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010912117.1"
                     /note="ObgE; essential GTPase; exhibits high exchange rate
                     for GTP/GDP; associates with 50S ribosomal subunit;
                     involved in regulation of chromosomal replication; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEU0158520.1"
                     /translation="MKFLDQAKIYIRSGDGGAGAVSFRREKFIEFGGPDGGDGGKGGD
                     VWAECVAGLNTLIDYRYQQHFKAQNGRSGMGKNRAGAGGADKLIALPPGTQIYAEDQA
                     TLIADLVKVGDRARLARGGNGGFGNAHFKSSTNRAPRRANPGAPGEELTIWLRLKLIA
                     DAGIIGEPNAGKSTFLASVSAAKPK"
     gene            complement(580..1167)
                     /locus_tag="VFR00_04370"
     CDS             complement(580..1167)
                     /locus_tag="VFR00_04370"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU0158521.1"
                     /translation="MFTIKTQRLLLRPFQRSDAREFARLAGDWAVASMTSDIPHPFDE
                     AQALAWLKPARGEVRFAIEAEAGRLIGGAGFYRRPSGVAELGFWLGKPWWGQGYATEA
                     GRAVLAHGIRDRRLPAFSSAHFVDNPASGRVLAKLGFVEVERIPIACTARGYQVDAVT
                     YRLERQRALLVIAPTGDGLAERRRWRAWLGRIGAG"
     gene            complement(1230..1502)
                     /gene="rpmA"
                     /locus_tag="VFR00_04375"
     CDS             complement(1230..1502)
                     /gene="rpmA"
                     /locus_tag="VFR00_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004676172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEU0158522.1"
                     /translation="MAHKKAGGSSRNGRDTAGRRLGIKRYGGERVLAGNILVRQRGTR
                     WHPGSNVGMGTDHTLFALTEGSVEFATKRNGRIFVSVKPAAKEAAE"
     gene            complement(1529..>1697)
                     /gene="rplU"
                     /locus_tag="VFR00_04380"
     CDS             complement(1529..>1697)
                     /gene="rplU"
                     /locus_tag="VFR00_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002967949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEU0158523.1"
                     /translation="GTPGIAGAKVTAEIVEQGRAPKVIAFKKRRRKNSRRKRGHRQEQ
                     TTVRIKDIVGA"
BASE COUNT          272 a          568 c          577 g          280 t
ORIGIN      
        1 tcttgggctt ggcggcgctg acgctggcaa ggaatgtgga cttgcccgcg ttgggttcac
       61 cgataattcc ggcatcggcg atcagcttca aacgcagcca gatcgtcaac tcttcgcccg
      121 gtgcgcccgg gttagcgcgg cgcggcgccc gattggtcga ggatttgaag tgggcgttgc
      181 cgaagccgcc gttgccgccg cgcgcaaggc gggcgcggtc accaactttg acgagatcgg
      241 cgatgagggt agcctgatcc tccgcgtaga tctgtgtgcc cggtggcagc gcgatcagct
      301 tgtccgcacc gccggccccg gcgcggttct tgcccatgcc gctgcggcca ttctgggcct
      361 tgaaatgctg ctggtagcga tagtcgatca gggtattgag accggccacg cactcggccc
      421 aaacgtcgcc acccttaccg ccgtcgccgc catcggggcc gccgaactcg ataaactttt
      481 cgcgccgaaa ggacaccgca ccggcaccgc cgtcgccgga gcggatgtag atcttggctt
      541 gatccaaaaa cttcatgggc tccgcccgcg gcaggccgct caaccggcgc cgatgcggcc
      601 gagccaggcg cgccagcgcc gccgctccgc cagaccatcg ccggtgggtg cgatcaccaa
      661 tagggcgcgc tggcgctcga gccgataggt aacggcgtcg acttggtagc cgcgcgccgt
      721 gcaggcaatg gggatgcgct ccacctcgac gaagccgagc ttggccagga cgcggccgga
      781 ggcagggttg tcgacaaaat gagcggacga aaaggccggc aagcgccggt cacggatgcc
      841 atgggccagg acagcacggc cggcttccgt cgcatagccc tggccccacc agggtttgcc
      901 cagccagaag ccgagttcgg cgacgccgga gggacggcgg tagaagcccg cgccgccgat
      961 caggcgcccg gcctctgctt cgatggcaaa ccgcacctcg cccctggccg gcttgagcca
     1021 ggccagcgcc tgggcctcgt cgaagggatg aggaatgtcg ctcgtcatcg aggcgaccgc
     1081 ccagtcgccg gcgaggcggg caaactcccg agcatcggag cgttggaagg gccgcaggag
     1141 caggcgctgg gttttgatcg tgaacatgcg gccggcgtct atgtgcgcgc gccccgagcc
     1201 tggtgcctca gccggcaagc cggcgggcgc tattcggcag cctctttggc cgccggtttc
     1261 accgacacga agatgcgtcc gttgcgcttg gtggcaaact cgacgcttcc ctcggtcaag
     1321 gcaaacagcg tgtggtccgt acccatgccg acgttcgaac cggggtgcca gcgggtgcca
     1381 cgctggcgca cgagaatgtt gccggcaagc acacgctcac caccataccg cttaatgccg
     1441 agccgccgcc ccgccgtatc gcggccgttg cgcgaggagc cgcctgcctt tttgtgtgcc
     1501 atggaaagga tccttgctga gccggcagtc aggcgccgac gatatccttg atacgaacgg
     1561 tggtctgctc ctgccgatgg ccgcgcttgc gtcgcgagtt cttgcgccgc cgcttcttga
     1621 aagcgatgac cttgggggcg cggccctgct cgacgatttc cgccgtcacc ttggcgccgg
     1681 cgatgccggg ggtgccg
//
LOCUS       DASTMT010000193         2990 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_432067, whole genome shotgun sequence.
ACCESSION   DASTMT010000193 DASTMT010000000
VERSION     DASTMT010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2990)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2990)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2990
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_432067"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..780)
                     /locus_tag="VFR00_04385"
     CDS             complement(<1..780)
                     /locus_tag="VFR00_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEU0158524.1"
                     /translation="MTRHHHAHDAGHAHAHHAGHAHVNNERRVGWAAILTATYMLAEV
                     AGGLMAGSLALLADAGHMLADAASLALAWAAFRIARRPSDWQRTYGFHRFQVIAAYTN
                     GITLIFIAAAVLWEALDRWREPVTVLGGPMLLIASLGLLVNIAAFLALHGAERDNLNI
                     RGAMLHVLGDLAGSAAAIVAALVILWTGWTPIDVLLSALVALLILRSAWGVVAESGRI
                     LIEAAPKGLDVRALARDLEASIPGLEDVHHVHAWSLTEARPM"
     gene            complement(953..2206)
                     /locus_tag="VFR00_04390"
     CDS             complement(953..2206)
                     /locus_tag="VFR00_04390"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019196336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEU0158525.1"
                     /translation="MPHYVRLFWVLFVLAAAPAAADRLATGTPASVGFASDRLQRLTE
                     VLQSEIGKGTMPGAVLMIQRNGKVAYFEALGVQDPERKTPMRQDTIFRIYSMSKPITT
                     TAAMMLVEQGRLALADPVQKYLPEFKDVQVGEEKPDKTLELVAPRRPMTVQDLMRHTA
                     GLTYGFFGEGAVKKAYREANLDAGDPTTAEFVARLAKLPLVYQPGTTWDYSHATDVLG
                     RVVEVVAGKPLSAALREMLFLPLGMTDTSFYLEDPSKYPRLAEPFAADRSIGAGAVLN
                     DPREVKKYESGGGGLLSTTADYARFLSMLANGGELDGKRYLSPKTVAYMTSDHMGDVI
                     KRGPYDLVGPGYKFGLGFAVRTEPGVSPLAGSPGDFTWGGAAGTYFWVDPKEKMFAIF
                     MMQAPSKRGPLRPLLRDMVYAAMVE"
     gene            complement(2285..2890)
                     /locus_tag="VFR00_04395"
     CDS             complement(2285..2890)
                     /locus_tag="VFR00_04395"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEU0158526.1"
                     /translation="MSDSIIYHDGNRRLQDAFQSRPIADRLEAKLARSQFSPQDRALI
                     EAAIYFFIATADADGRPDCSFKGGPAGFVRVTGADELAFPDYDGNGMFKSLGNVLVNP
                     SVGLLFIDMHGAGRRLRVNGEARIVQGDPLLAATVGAQLMVRVKARAIFPNCPRYIPK
                     LSLLAASPFTPTPGADPVEPAWKGFDDFKDVVHPRQPTTKG"
BASE COUNT          532 a         1002 c          993 g          463 t
ORIGIN      
        1 catcgggcgc gcttccgtca gcgaccaggc gtggacgtga tgcacgtcct ccaggccggg
       61 aatgctggcc tcgaggtcac gcgcaagggc gcgcacatcg agccccttcg gggcggcctc
      121 gatcaggatg cgccccgact cggcaaccac cccccaggcg ctgcgcagga tcaacagggc
      181 aaccaaagcc gagagcagca catcgatcgg ggtccagccg gtccataaaa tgacgagggc
      241 ggccacgatg gccgcggccg atcccgccaa gtcgccgagc acgtggagca tggctcccct
      301 gatattgagg ttgtcgcgct ccgcgccgtg caacgccaga aaggccgcga tattgacaag
      361 gagcccgagg ctggcaatca gcagcatcgg cccacccagc accgtgacgg gctcgcgcca
      421 gcgatcgagc gcctcccaca gcaccgcagc ggcaatgaag atcaaagtga tgccgttggt
      481 ataggccgcg atcacctgaa agcggtgaaa gccgtaggtt cgttgccaat cggagggccg
      541 ccgcgcgatc cgaaacgcgg cccatgccag cgccagggag gccgcatccg ccagcatgtg
      601 cccggcatcg gccaacaggg ccagcgaccc cgccatgagg ccgccggcga cttccgccag
      661 catgtaggtg gccgtcagaa tggccgccca gccgacgcgc cgctcgttat tgacgtgggc
      721 atgtcccgcg tggtgggcgt gggcgtggcc cgcatcgtgg gcatggtgat gtcttgtcat
      781 acactcccca ccgctggctg gggcgcggct cgggcgacat cgcgcgcgat cagcatatag
      841 gccacgcgcc gccgtcgcga gccgcacggc gcgcctggcg aaccctcggc ccaatctgcg
      901 cggctcaggc ggtgccggcg gctgagcgcc gccggcggct tgccggtgcc gcttactcca
      961 ccatggccgc ataaaccata tcccgcagca gcgggcgcag gggtccgcgt ttgctcggcg
     1021 cctgcatcat gaagatggcg aacatctttt ccttgggatc gacccaaaag taggtgcccg
     1081 ccgcgccgcc ccaggtgaaa tcgcccggcg agcccgccaa cggcgacacg ccgggctccg
     1141 tgcgcacggc aaatccgagg ccaaacttgt agcccggtcc gaccaggtca tagggcccgc
     1201 gcttgatgac gtcacccatg tggtccgagg tcatgtaggc cacggtcttg gggctcaaat
     1261 aacgcttgcc gtcaagctcc ccgccgttgg ccagcatgct cagaaagcgg gcatagtcgg
     1321 ccgtcgtgga caggaggccg ccgccgccgg actcgtattt cttcacctcg cgtggatcgt
     1381 taagcacggc accggcgccg atgctgcggt cggcggcgaa cggttcggca agacgcgggt
     1441 acttgctggg atcctccagg tagaagctgg tatccgtcat gccgagcggc aagaacagca
     1501 tctcgcgcaa cgccgcggac agcggtttgc ccgccaccac ctccacgacg cgaccgagga
     1561 cgtcggttgc gtggctgtaa tcccaggtcg tgcccggctg atacaccagg ggaagcttgg
     1621 ccaagcgcgc gacgaattcg gcggtcgtgg gatcgcccgc gtcgagattg gcctcccggt
     1681 aggccttctt gacggcgcct tccccgaaga acccataggt caggcccgcg gtgtggcgca
     1741 tcaggtcctg cacggtcatc ggccggcgcg gcgccaccag ctcgagcgtc ttgtcgggct
     1801 tctcctcgcc aacctgcacg tccttgaatt ccggcaggta cttctggacg gggtcggcca
     1861 gcgccaagcg accctgctcc accagcatca tggcggcggt ggtcgtgatg ggcttggaca
     1921 tggagtagat gcggaaaatg gtatcctggc gcatgggcgt cttgcgctcg ggatcctgca
     1981 cacccaaggc ttcaaagtag gcaactttgc cattgcgctg gatcatgagc acggcaccgg
     2041 gcatcgttcc cttgccgatc tcgctttgca gaacctcggt caggcgttgc agccggtcgc
     2101 tggcgaagcc gacgctggcc ggcgtgccgg tggcaagccg atccgcggcg gcgggcgcgg
     2161 cagcgaggac gaacaggacc cagaagagac ggacgtagtg cggcatcagg gcgctccctc
     2221 ggtgttggcg atgggggctg cagcatgaca cggaaaggcg cggcggagaa cggcgcccac
     2281 cacctcaacc tttcgtcgtc ggctggcgcg ggtgcacgac atccttgaaa tcgtcaaagc
     2341 ccttccacgc cggctcgacc ggatcggcgc cgggcgtcgg cgtaaagggc gatgccgcca
     2401 gcagcgacag cttggggata tagcgcggac agtttgggaa gatggcgcgg gccttgaccc
     2461 tgaccatcag ctgggccccg accgtggcgg cgagtagagg gtcgccttgg acaatgcgag
     2521 cctcgccgtt gacgcgcaac cgccgacccg cgccatgcat atcgatgaac agcaggccga
     2581 cgctcgggtt gaccaggaca ttgccaaggc tcttgaacat gccgtttccg tcataatcgg
     2641 gaaacgccag ctcatcggca ccagtgacgc gcacgaagcc cgccggtccg cctttgaagg
     2701 agcagtccgg ccggccgtcg gcatccgccg tggctatgaa gaaataaatt gcggcctcga
     2761 tcagcgcccg gtcctggggg ctaaactggc tgcgcgccag ctttgcctcc agccggtcgg
     2821 cgatgggccg gctctggaag gcgtcttgca accggcgatt gccgtcgtga tagattatgc
     2881 tgtcgctcat gtcggacctc gctcgagccg caagcctccc ccaccccaag gccacccccc
     2941 gcgaccggcg gtcacagccc agggacggat acttacagcg cagcggagga
//
LOCUS       DASTMT010000194         5960 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_102064, whole genome shotgun sequence.
ACCESSION   DASTMT010000194 DASTMT010000000
VERSION     DASTMT010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 5960)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5960)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5960
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102064"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..258
                     /locus_tag="VFR00_04400"
     CDS             <1..258
                     /locus_tag="VFR00_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010975049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEU0158527.1"
                     /translation="IRTIEGMAQGDELHPLQQKFLEHAALQCGICTPGFLVAAKALLD
                     KTPDPSETEVRYWLAGNLCRCTGYDKIVRAVLDAAAELRGA"
     gene            262..2565
                     /locus_tag="VFR00_04405"
     CDS             262..2565
                     /locus_tag="VFR00_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010910804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEU0158528.1"
                     /translation="MQEKVKDGQSSEFQVVGTRPVRPDGADKVTGRAKFGADFSLPGQ
                     LVGKVLRSPYPHARIKSIDASAALKLPGVKAVVCRDDFVEQSSEFIPAGEMMINYRDV
                     VRNVMAREKVLYEGHPVAAVAATSAALAKEALRLIKVDYQVLPHVIDVVEAMRPDAPL
                     LHEDMFTAGVEPAPKTPSNVAKRLEIGHGDIAAGFRQAELVIEREFTTKPVHQGYIEP
                     HACVASVSEDGQADVWVSTQGHWVVRAHCARLLGWDIGKVRVTAAEIGGGFGGKTVVY
                     LEPIALALSKKARRPVKMVMTREEVFKASGPTSGAHVRVKIGAKKDGRIVAAEAELKY
                     QAGAFAGSPVQPGAMCAYAPYDIENVKVIGYDVVTNRPKVAAYRAPGGPISEFGVESV
                     LDEIAAKLGIDPIELRLKNAAKQGTKAAYGPKFGPIGMVETLEAAKAHAHWRTPLGKH
                     QGRGVASGFWFNIGGETSVSLSLNEDGTLSLTAGTPDIGGLRASLCMMAAEELGVPLD
                     KIRVQIGDTGQLGYNFLTGGSRSTFSSGMATVEAARDVMKEACKRAAKLWELPDDAVE
                     YNNGGVRPAGPNAGKHAPMTLKEIAGIAGKTGGPIVGYARINAAGAAPSLATHIADVE
                     VDPETGKVSVIRYTAIQDAGRAIHPSYVEGQYQGGVAQGIGWALNEEYIYGDDGKLQN
                     PGFLDYRVPVASDLPMIDTVIIEVPNPRHPYGVRGVGETPIVPPMAAIANAVANATGV
                     RFTDLPMSPPRVLKALDEARAKGSAKL"
     gene            2654..2899
                     /locus_tag="VFR00_04410"
     CDS             2654..2899
                     /locus_tag="VFR00_04410"
                     /inference="COORDINATES: protein motif:HMM:NF014636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HEU0158529.1"
                     /translation="MAHVTLIGNLRQFTGGVTEIEVAASNVHQLFARLGEKYPALLPH
                     LQTGSAVAIDGQIYQDALFQEIGPDSDVHILPQIAGG"
     gene            complement(2963..3493)
                     /locus_tag="VFR00_04415"
     CDS             complement(2963..3493)
                     /locus_tag="VFR00_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008510332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase family protein"
                     /protein_id="HEU0158530.1"
                     /translation="MTSSLRMLALASGGFLIATGIAIAQQLNVDINRLSESGVGDKIG
                     SVVVEEGKDGVSFKVSAEGIAKGAHGFHIHEKGDCAAATKDGKMVAGFAAGPHYDPAG
                     TKSHKGPKGGGHQGDLPILNASDKGIEQTVTAPQLKLADVRGRALMIHDGGDNYSDTP
                     ENGGGAGRIACGVVPK"
     gene            3844..5343
                     /locus_tag="VFR00_04420"
     CDS             3844..5343
                     /locus_tag="VFR00_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015666281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HEU0158531.1"
                     /translation="MDQVNDLVCLSAVELRRRIGRKEISPVEVLEASIDRIASIDPAV
                     NAIAARDYARARQQAKAAEGAVLRGEELGALHGLPTGVKDLSDTEGLLTTHGSPLYRD
                     HVPRHDAAMVARLRRAGAVIVAKTNTPEFGAGANTRNSVWGATGNPFDPLLNAGGSSG
                     GSAVALACDMLPLCTGSDMGGSLRIPAAKCGVVGMRPSPGVVPYEARPLGWSPLAVLG
                     PMGRTVGDLRLLFAAQVDVDAREPLAFPLDPGEIGRARPVDLGRLRVAWSEDFGQCPV
                     ALEIRQLIRARIASIRHLFRSCEEVEFDFGAADRCFDIMRAESFLARHQAAYARDPAS
                     LGPNVRANYELGAKMTLADAAWGHAEQTRLFHAFQAQFRDYDLVLSPTTPVSPFPWHQ
                     LYLAELDGKPLRNYYHWLALTYYISLVGNPAMSLPCGTDAHGMPFGLQVIGKFRGDVE
                     LLNAAEALEHACSGIAELRRPRPDLAKLRGITTAALKAIVTHPPHLPTH"
     gene            5362..>5960
                     /locus_tag="VFR00_04425"
     CDS             5362..>5960
                     /locus_tag="VFR00_04425"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEU0158532.1"
                     /translation="MHGKRPTLTSLHGLVAAAHPLAAQAGAKLLASGGNAFDAAAATA
                     AALNIVEPFMSGLAGLGYATCYIGAERRVRVLDFVPPVPSRLPVERFSKREDLQRGPL
                     SVGIPGNLAGWAELVRAYGKKPLADALAPAIALARDGFALAEFGVAEFNEQIPLLRRC
                     TAFYEDWARTYTGGAQGLALGHILRQPELAATLEEIAAKG"
BASE COUNT         1000 a         1999 c         1934 g         1027 t
ORIGIN      
        1 atccgcacca tcgagggtat ggcgcaggga gatgagttgc acccgctgca gcaaaaattc
       61 ctcgagcatg cggccctgca gtgcggcatt tgcactccgg gttttctggt ggcggccaag
      121 gccctgctcg acaaaacgcc cgatccgtca gaaaccgagg tgcgctactg gctagccggc
      181 aatctgtgcc gctgcaccgg ctatgacaag atcgtgcgcg ccgtactcga cgccgccgcc
      241 gagctgagag gagcctgagc gatgcaagag aaagtcaagg acggccagag ttccgagttt
      301 caggtggtcg gcacgcgccc cgtacgtccg gacggcgccg ataaggtcac gggccgcgcc
      361 aagttcggcg cggacttcag cctgccgggc cagctcgtcg gcaaggtgct gcgcagtcct
      421 tatccgcacg cacgcatcaa gtcgatcgat gcctccgcgg cgctgaagct gccgggggtc
      481 aaggccgtcg tctgccgcga tgatttcgtt gagcagtcct ccgagttcat ccctgccggc
      541 gagatgatga ttaactatcg cgacgtcgtc cgcaacgtga tggcccggga aaaggtcctg
      601 tacgaaggcc atcccgtcgc cgcggtggcg gccaccagcg cggcccttgc caaggaagcc
      661 ttgcggctca tcaaggtcga ttaccaggtg ttgccgcacg tcatcgacgt tgtcgaagcc
      721 atgcgtcccg acgcgcccct cctgcatgag gacatgttta cggccggcgt cgagcccgcg
      781 ccgaagaccc cctccaacgt cgccaagcgc cttgaaatcg ggcacggcga catcgcggcc
      841 ggctttaggc aagccgagct cgtgatcgag cgcgagttca cgaccaagcc cgtgcaccaa
      901 ggctacatcg aaccacatgc gtgtgtggca agcgtatctg aggacgggca ggccgacgtc
      961 tgggtgtcga cccagggcca ctgggtggtg cgcgcgcact gcgcgcggct gctcggctgg
     1021 gacatcggca aggtgcgcgt gacggcggcg gagatcggcg gcggcttcgg cggcaaaacc
     1081 gtcgtctacc tggagccgat cgcgctcgcg ctctccaaga aagcgcgtcg tcccgtcaag
     1141 atggtgatga cgcgagagga ggttttcaaa gcctcaggac cgacctcggg agcgcacgtg
     1201 cgcgtcaaaa taggcgccaa gaaggacggg cgcatcgtgg cggccgaggc cgagctcaag
     1261 tatcaggccg gcgccttcgc cggatcgccg gtgcagcctg gcgccatgtg cgcctatgcg
     1321 ccctatgaca tcgagaacgt caaggtgatc ggttacgatg tggtcaccaa ccggccgaag
     1381 gtcgcggcct atcgcgcgcc cggcggcccg atatccgagt tcggcgtcga gagcgtgctc
     1441 gacgagatcg ccgccaagct cggcatcgat ccgatcgagt tgcgcctgaa aaacgcggct
     1501 aagcagggca ccaaggcggc ctacggcccc aaattcgggc cgatcggcat ggtggagacc
     1561 ctggaggcgg ccaaagcgca tgcgcattgg cgcacgccgc tcggcaaaca ccagggtcgc
     1621 ggcgtggcca gcggcttttg gttcaacatc ggcggcgaaa cttccgtctc gctgtccctc
     1681 aacgaggatg gcaccttatc gctcacggcc ggcacgccgg acatcggcgg cctgcgtgcc
     1741 tcgctgtgca tgatggcggc ggaagagctg ggcgtgccgt tggacaagat ccgggtgcaa
     1801 atcggcgaca cgggtcagct cggctacaac ttcctgaccg gcggcagccg ttcgacgttc
     1861 tcgagcggca tggccaccgt ggaagccgcg cgcgacgtca tgaaggaggc ttgcaagcgc
     1921 gccgcaaagc tatgggaatt gcccgacgat gcggtcgaat acaacaacgg cggcgtgcgt
     1981 ccggccgggc ccaatgccgg caagcatgcg ccgatgacgc tgaaagagat cgccggcatc
     2041 gccggcaaga ccggcggtcc gatcgtcggt tatgcccgca tcaacgccgc cggcgctgcg
     2101 ccgagcctcg ccacgcatat cgccgacgtc gaggtcgatc cggagaccgg caaggtctcc
     2161 gtcatccgct atacggcaat tcaggatgct ggcagggcca ttcatcccag ctacgtggag
     2221 ggccaatacc agggcggggt cgcgcaaggc atcggctggg ccctcaacga ggagtacatc
     2281 tacggcgacg acggcaagct gcagaacccg ggcttcctgg attaccgcgt gcccgtggcc
     2341 tccgacctgc cgatgatcga taccgtgatc atcgaggtgc cgaacccgcg ccatccctac
     2401 ggggtgcgcg gggtgggcga aacgccgatc gtgccgccga tggcggccat tgccaatgcg
     2461 gtcgccaacg ccaccggcgt gcgctttacc gatctgccca tgtcgccgcc gcgggtcctg
     2521 aaagccctcg acgaagcgcg cgccaagggc tccgccaagc tttgatgcca acccgggcgg
     2581 gctctcgaag gaggagcccg ccccgctgcc ggccccgcgt tccggctggg acgtgccggc
     2641 caggacattg cccatggccc acgtgacgct gatcggaaat ctgcggcaat ttacgggggg
     2701 cgtgacggaa attgaggtcg cggccagcaa cgtgcatcaa ctctttgcac gactgggaga
     2761 aaagtacccc gccctgctgc cgcacttgca gacgggatcg gccgtcgcca tcgacggcca
     2821 aatctaccag gacgcgctgt tccaggagat tggccccgac agcgacgtgc acattctgcc
     2881 gcagatcgct ggaggatagc gcgggctacg tgtcccggcg ttacccttct ttccccaact
     2941 gggcgctttc ctggcgaggt ccctatttcg gcaccacgcc gcaggcaatg cgtcccgcgc
     3001 caccgccgtt ttcgggcgtg tcgctgtagt tgtcgccgcc gtcgtggatc attagggcgc
     3061 gcccgcggac atcggcaagc ttcagttgcg gcgccgtcac cgtctgctcg atgcctttgt
     3121 cgcttgcatt caagatgggc aggtcgccct ggtgcccgcc ccccttcggc cccttgtggg
     3181 atttcgtgcc ggccggatcg taatgcggtc cagcggcaaa gccggccacc atcttgccat
     3241 ccttggtggc ggcggcgcag tcacccttct cgtggatgtg gaaaccatgc gcccccttgg
     3301 caatgccctc ggcggatacc ttgaaggaaa cgccgtcctt gccttcctcg acgacgacgg
     3361 agccgatctt atcgccgaca ccgctctcag acaggcgatt gatatccaca ttcaactgtt
     3421 gagcgatcgc gatcccggtc gcaatcagaa atccgccgct ggctagggca agcatgcgta
     3481 gagagctcgt catggcgcaa cctcgactgg cgtggcttgg gccatgaacg gtaccgcaac
     3541 cgagtttgtt ccgcctggca ggttccgcgc ggccacgcat gagcaatcgg agtgctccag
     3601 gcaagcttgc gcatggcctt ggccggcgtc tgccccgaga gaaaaatgcc acgcacaccc
     3661 gggcgcgagg cctcattttt gtgagcgcgc gtgtcgcgca gtttcgtttg ccgccgtcga
     3721 cacttaggcg ccgcgcggac tatccttggt ttgtgccgca aggcggcaat tgccttttgc
     3781 gcgcgccgcg gtgacactcc gcgctagcga tggttgcggc gggaattcgc cagaggcacc
     3841 ggcatggacc aagttaacga tcttgtttgc ttgagtgccg tggaattgcg ccggcgcatc
     3901 ggccgcaagg aaatctcgcc agtggaggtc ttggaggcct cgatcgaccg catcgccagc
     3961 atcgatccgg ctgtcaatgc aattgctgct cgcgattatg cgcgagcccg gcagcaggcc
     4021 aaggcggccg agggcgcggt cttgcgcggc gaggaactcg gcgcgctgca cggcctgccg
     4081 accggggtca aggacttgag cgacaccgaa ggtttgctga ccacccacgg ctcgccgctc
     4141 tatcgcgacc acgtgcccag acacgacgcg gccatggtcg cccgcttacg ccgggccggt
     4201 gcggtgatcg ttgcgaagac caatacgccc gagttcgggg ccggcgccaa cacgcgcaat
     4261 tcggtgtggg gcgccaccgg caacccgttc gatcccctcc tcaatgccgg cggctcctcc
     4321 ggtggttcgg cggtggcgct cgcctgcgac atgcttcccc tctgcaccgg ctcggatatg
     4381 gggggctcct tgcgtattcc ggcggccaag tgtggcgtgg tcggcatgcg gccctctccc
     4441 ggcgtggtcc cctacgaggc acggcccctg ggctggtcac cgctcgccgt gctcggcccg
     4501 atgggccgga cggtggggga tttgcgtctt ctgtttgcgg cccaggttga cgtggacgcg
     4561 cgcgaaccat tggcttttcc gctcgatccg ggcgagatcg ggcgcgcccg gccggtcgac
     4621 ctcggccggc tgcgcgttgc ctggagcgag gatttcggtc aatgccccgt ggccttggaa
     4681 atccgccaac tgattcgggc ccgtatcgcc agcatccggc acttgttccg atcgtgcgag
     4741 gaggtcgaat tcgattttgg cgccgcggat cgctgcttcg acatcatgcg ggccgagagc
     4801 ttcctggcac gccatcaggc cgcctacgca cgcgaccctg cctcgctcgg gcccaacgtg
     4861 cgtgccaact acgagctcgg cgcaaaaatg acgctggccg acgccgcctg gggccacgcc
     4921 gagcaaacgc ggctgttcca cgccttccag gcgcagtttc gcgactacga tctcgtccta
     4981 tcgccaacca cgccggtatc gcccttcccc tggcatcagc tttacctcgc cgagctcgac
     5041 ggcaagccgc tgcgaaacta ctaccactgg ctagcgctca cctactacat tagcctcgtc
     5101 ggcaatccgg cgatgtcgtt gccctgtggc accgatgcgc acggtatgcc ctttggattg
     5161 caagtgatcg gtaaattccg tggcgatgtc gagctcttga acgcggccga agcgcttgag
     5221 catgcctgca gcggcattgc cgagctccgc cggccacgac cggatctcgc caagctgcgc
     5281 ggcatcacca ccgcggccct gaaagcgatc gtcacccacc cgccccatct tcccacccac
     5341 tgacaattgg ttggcagcac catgcacggc aaacggccga cccttacctc cctccacggc
     5401 ctggtcgcgg cggcgcatcc gctggccgcg caagccggcg ccaagctgtt ggccagtggc
     5461 ggcaatgcct tcgatgcggc ggctgccacg gcagcggcgc tcaacatcgt ggagcctttc
     5521 atgtccgggc tcgccgggct cggttacgcc acgtgctaca tcggcgccga gcgccgcgtg
     5581 cgcgtgctcg acttcgtgcc gccggtgcct tcgcgcctgc cggtcgagcg ctttagcaaa
     5641 cgcgaggatc tgcaacgcgg gcccctgtcc gtcggcatac ccggcaatct cgcgggctgg
     5701 gcggagctcg tgcgcgccta cggcaagaag ccgttggccg acgcgcttgc cccggccatt
     5761 gccttggcca gggatggttt cgcgctcgcc gaattcggtg tcgccgaatt caacgagcaa
     5821 attccgttgc tgcggcgctg cacggccttt tacgaggact gggcgcggac ctataccggc
     5881 ggggcgcaag gcctcgcgct cgggcacatc ctgcgccaac cggagctcgc cgcgacgctc
     5941 gaggagatcg cggccaaagg
//
LOCUS       DASTMT010000195        11790 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_3056101, whole genome shotgun sequence.
ACCESSION   DASTMT010000195 DASTMT010000000
VERSION     DASTMT010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 11790)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11790)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11790
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3056101"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..692)
                     /locus_tag="VFR00_04430"
     CDS             complement(<1..692)
                     /locus_tag="VFR00_04430"
                     /inference="COORDINATES: protein motif:HMM:NF015131.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c peroxidase"
                     /protein_id="HEU0158533.1"
                     /translation="MALLGDAERASVAAAQDPLAGLKAKFARPSFVPHPADNAPTAAK
                     VALGERLFFDKEISATGTIACASCHDPKLAFSDGEPTGRGVSGRRLVRHTPSLWNVAW
                     SRLLFWDGRASSLEAQVRFPVEHPDEMGDSLENAVLRLSRHESYEGAFAAAFPRDPEI
                     TPRNVALALAAYERTLISPPTRFDAFIAGDATALSAGEMRGFRIFTGKGRCSNCHTGF
                     ALTDNNFYDIGLP"
     gene            complement(776..1603)
                     /locus_tag="VFR00_04435"
     CDS             complement(776..1603)
                     /locus_tag="VFR00_04435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0158534.1"
                     /translation="MAKLSRKLWISIGAAGIVSAPLMPLAAQQGAHKSHPAAASQAPQ
                     AASTSATEGGEAYLTDGGPKDTRIRFYRDIALMRGHLLVGDQLIAQGLWDEALPHFLH
                     PTEELYALIEKYIKLHRITPFDRDLKALAQTVKAKRKAAYEERARVVDQRLSNALEAA
                     KRFMTPPLRSFTVRSALAVLRVAQSEYEEAIEDGKFTKPVEYQDSRGFVWQSERMLTG
                     IASELKGIDAEGLAKLRADFERLKAAWPELLPPAAPLLTPAEIAALVSDIERSAARF"
     gene            1742..2401
                     /locus_tag="VFR00_04440"
     CDS             1742..2401
                     /locus_tag="VFR00_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019402309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-dependent dioxygenase"
                     /protein_id="HEU0158535.1"
                     /translation="MILAIAELLSAADLEAVRTELAGAAFGDGRATAGWSARLVKQNL
                     QARDGPGTQRARSLIETRLLEHTVFQLATRPKRLIGPLISRYEPGHAYGVHVDDALLD
                     GVRSDVAFSLFLSAPESYEGGELVIETAAGEEAFKLPAGSLVTYPATFLHRVAPVSHG
                     SRLVAAGWVRSFVRDAAHRELLFDLDTARRRLFDRDGKSAEADLLAKCTANLLREWCD
                     D"
     gene            complement(2421..2915)
                     /gene="bfr"
                     /locus_tag="VFR00_04445"
     CDS             complement(2421..2915)
                     /gene="bfr"
                     /locus_tag="VFR00_04445"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HEU0158536.1"
                     /translation="MKGKKEVIDILNDALKHELGAVNQYWLHYRMLDNWGYTKLAKKE
                     RKESIEEMQHADKLVDRIIFLEGLPNMQHIAPLMIGQDIKEVLECDLRGEFNARDLYT
                     RAREICRRNEDLVSMALFEELLADEEGHIDFLETQLDLLSKIGVQNYGQLQADAADEA
                     EGDD"
     gene            complement(3073..3432)
                     /locus_tag="VFR00_04450"
     CDS             complement(3073..3432)
                     /locus_tag="VFR00_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0158537.1"
                     /translation="MIICSCAAVSDADIERALIEILSLPEAPLPTPGVVYRHLQKKMA
                     CCGCSPLAVSTIYEKIDLLAAKGVVCPYACACAQGRLLRCAGDSPAQRLIERTPPRPW
                     RTEIPLEAGAGPMLETA"
     gene            complement(3443..3955)
                     /locus_tag="VFR00_04455"
     CDS             complement(3443..3955)
                     /locus_tag="VFR00_04455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0158538.1"
                     /translation="MRRLGAAVLGDDTLPIAAIAEPERLVGLGFRYWLNGFRTGDIGC
                     WEKAWDAYAGSLGPALAKGAVSDLARWVRSIDRHAQRRPETLATDCDCFCRDECVAIA
                     MIAACQHNACPAMRACAFSLLGCSLIEDVVSGATSFAATMRGAKQVLPPCYTTDASLL
                     AMAPATAARQ"
     gene            complement(4246..4740)
                     /locus_tag="VFR00_04460"
     CDS             complement(4246..4740)
                     /locus_tag="VFR00_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEU0158539.1"
                     /translation="MRLTKSTGHAIRILLDCAGADDRLIKVAELSERLQITQQNVFKI
                     VNLLARAGLIAAVRGRNGGVRLARRADQIRIGDVVRATEITHVELEDCAPARRQRSRS
                     GVNEILDDALGAFIDVLDQHTLADMVRTAPRASAQARRRNSGAARGAGMAASRYRRSL
                     QTSN"
     gene            complement(4913..5092)
                     /locus_tag="VFR00_04465"
     CDS             complement(4913..5092)
                     /locus_tag="VFR00_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018182403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HEU0158540.1"
                     /translation="MSSDEDPQRPPGAAAPAGDRRRRLKVSELLQGEREVILEHDGHD
                     YRLRITANGKLILTK"
     gene            complement(5287..6255)
                     /locus_tag="VFR00_04470"
     CDS             complement(5287..6255)
                     /locus_tag="VFR00_04470"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEU0158541.1"
                     /translation="MRLLALILLGLSTLAATAAAETYPDRPVKVVVGFAAGGPTDVVA
                     RIICDKLSAKLGKQFYVLNQPGAGSNTASGMVAASPPDGYTLTVVSTGFIINPSLFAK
                     VPYDPVKDFAPISIVAVSPNVVSVHPSVPAKSMRELIEVIKANPGKYSFAAPGVGSTP
                     HLSGEIFRLANDLDLPTVQFTGAGPAMQSTVGGHTPVAFSAVPPAAPQIQQGLLRGLA
                     VTAEKRSKALPDVPTMAEAGIPGQEAYTLTGILAPAGTPKALIELLHREIVALVALPD
                     VETRLDELGFEVVASSPEEFAQRIKTEREKWSQVIRDAKIKAQDAK"
     gene            6611..7108
                     /locus_tag="VFR00_04475"
     CDS             6611..7108
                     /locus_tag="VFR00_04475"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HEU0158542.1"
                     /translation="MFTRNPSAQPNTLVQPSPPSARPATPAVVNGAQRRTGPTESGDK
                     SIIGNDLRIIGQGLRIISRSILQIDGEIEGDVQAAEVIVGEQGKVSGLVAGQQVVVSG
                     KVSGAICAKSVTLQSTSEVEGDIHHMSFAIEQGAVFEGRSRRAATEADLNAVIDAKGG
                     QSRPA"
     gene            complement(7164..7445)
                     /locus_tag="VFR00_04480"
     CDS             complement(7164..7445)
                     /locus_tag="VFR00_04480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0158543.1"
                     /translation="MANSRAGIGVASLALLAASSLAQAQSEQKPSAPMKTSKDRAVIA
                     ADTTQAAPSKEKKLSMCLETWDAQTHMTKKEWRVACLRSVRDYPDAFDR"
     gene            8307..9083
                     /locus_tag="VFR00_04485"
     CDS             8307..9083
                     /locus_tag="VFR00_04485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0158544.1"
                     /translation="MTVIVVTQDRGVRGPQLAARIAAGLGLDLVSQEQLGHRVAARMH
                     INEETLRRLIEAPPPLFTRWLGERRRLAFYTAEEVATLARQGDVVIESWKPVAAVTGI
                     RHGIRVHIGEPRRAVRLGTGTSCCQAAISPLEAPLARRWGLDGAQAGSIVCDLVLAPA
                     PEALESCLTQLRQFAFGLPQQAPHALEETFAAATDEAAAVSAPATRWRRARGTEVDIG
                     SERMPLDGVTSPEQAIARIEERLHGRRRPPRRLPLPPDML"
     gene            complement(9098..9814)
                     /locus_tag="VFR00_04490"
     CDS             complement(9098..9814)
                     /locus_tag="VFR00_04490"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEU0158545.1"
                     /translation="MSADPSFIDRVRPFLRNNTFFGGLPDAALDALIRRGHTKKFARG
                     DVICRRGEAGDSVMVLLQGRIKVSNVNVDGKEVVLNFLGVGDITGEIGALDGNERTAD
                     AVALEPCEMFAVHARDLLPSLTAHPAALLEIIQILCEKLRSTSAIIEDNTLEMRARTA
                     RGLLRLARQHGRTGKNGVRLQLTMSQTELGSYLSISRENVSRQLGRLRDANVITVNEQ
                     QIIITDADGLAEIAATPSRD"
     gene            10271..11608
                     /locus_tag="VFR00_04495"
     CDS             10271..11608
                     /locus_tag="VFR00_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014495211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0158546.1"
                     /translation="MRSTTLTRRRLLATSAAASAMVAMPHVRGAHAAGKLTVGLWDHW
                     VPTANAASTALIEEWAAKERVEVQIDYITTQGNKLLLTEQAEAQSRTGHDIFTFRCWG
                     PADHAKLLEPLDDVMEPLIQQNGAANATAQYLGKSEGHWVAVPATRGSNVKGPCSRID
                     LLKKHAGIDVQAMYPAGGEPKAEAWTLDAFLRAAEACHKAGFPFGIGLGTTTDSVDSA
                     GAIFDAFGAQLVDAQGKVTVKTDAVRQALDYYTRLARFFPPDAPAWDDASNNKYLIAG
                     KGSLILNPPSAWAVAKRDAPQVAEQLWTHGMPAGPKGRFTPCIPYFWGVWSFGKNKSA
                     AKNLLTFLSQPPAVEKMVAASQGFDIPAFANLTQLKTWAEEGPPKGTLYHYPNRNEHQ
                     IVSIAGAPAPPKIAVQIYVQGIMTKMTVRHLQGEPMEKVLAWAETEVEGFLRT"
BASE COUNT         1994 a         3819 c         3947 g         2030 t
ORIGIN      
        1 ggcaggccga tatcgtagaa gttgttgtcg gtgagggcga accccgtgtg gcaattgctg
       61 cagcggccct tgccggtaaa aatgcgaaac ccccgcattt ccccggccga caaggcggtg
      121 gcgtcgcccg caatgaaggc atcgaagcgc gttggcgggg agatgagggt tcgctcatag
      181 gcagcgagcg cgagcgcgac gttgcgcggg gtgatttccg gatcgcgcgg gaaggcggcg
      241 gcgaaggcgc cctcgtagct ttcgtgccgc gacagtcgca acaccgcatt ctccaggctg
      301 tcgcccatct cgtcggggtg ctcgacggga aagcggacct gagcctccag gctcgatgcg
      361 cgcccgtccc agaacaacaa tcggctccag gccacattcc agaggctcgg ggtatggcgt
      421 accaggcgac gtccgctgac gccgcgcccc gtcggctccc catcgctgaa ggcgagcttg
      481 gggtcgtgac aggaagcgca ggcaatggtg ccggtggcgg agatctcctt gtcgaagaac
      541 aggcgttccc cgagagcgac cttcgctgcg gtcggtgcat tgtcggcggg gtgtggcacg
      601 aagctcgggc gcgcgaattt ggccttgagc cccgcgagcg gatcctgcgc ggccgcgacg
      661 ctggcgcgct cggcgtcacc gaggagggcc acgctgccga aagcgacgcc cgcacccagc
      721 gcaaacgcga gccaagcccg ccgccgcggc gcgcaaggac cgtgccggcc ggccatcaaa
      781 acctcgcggc gctgcgctcg atatcggaga cgagcgcggc gatctcagcg ggagttagca
      841 gcggcgccgc cggcggcagc agctccggcc aggccgcctt caggcgctcg aaatcggcgc
      901 ggagcttggc gagcccctcg gcgtcgatgc ccttgagctc gctggcgatc cccgtcagca
      961 tccgctccga ctgccaaacg aacccgcggc tgtcctgata ctccaccggc ttggtgaatt
     1021 tgccgtcctc gatggcttcc tcgtattcgg attgggcgac ccgcaacacg gcgagcgcgc
     1081 tgcgcacggt aaagctgcgc agcggcggtg tcatgaagcg cttggctgcc tcgagcgcat
     1141 tagacaggcg ttggtcgacg acgcgggcgc gctcctcgta ggccgccttg cgcttggctt
     1201 tcacggtctg ggccagcgcc ttcagatcgc ggtcgaaggg cgtgatgcgg tgcagcttga
     1261 tgtatttctc gatcagggcg tagagctctt cggtcgggtg caggaagtgc ggcaaggcct
     1321 cgtcccaaag accctgggcg atcaactggt cgcccaccag gaggtgccct cgcatcaggg
     1381 caatgtcgcg atagaagcga atgcgcgtgt ccttgggacc gccgtccgtc aggtaggctt
     1441 ccccgccctc cgtcgcactc gtgctggcgg cttggggggc ttggctcgct gcggcaggat
     1501 gggatttgtg cgcgccctgt tgggctgcca acggcatgag cggcgcgctg acgatgccgg
     1561 cggcgccgat cgagatccaa agcttgcggc tcagcttcgc catgcggggg gtactcctgg
     1621 ccaatgcggg aattgagcgg cgcgcgccga agtggtcttg cacgccgatc ttgcacgttg
     1681 gtcttgcagg ttgcgcgccg ctgccggaag gttgcgcctg agggggacta gggacacggc
     1741 catgatcctt gcgattgccg agctattgag tgcagctgac ctcgaggccg tccgcaccga
     1801 gctggccgga gcagccttcg gcgacggcag ggcaacggct ggctggtcgg cgcgcttggt
     1861 gaagcaaaat ttgcaggcgc gcgacggtcc cgggacgcaa agggcgcgca gcctgatcga
     1921 aacgcggctc ctcgagcata ccgtgttcca gctggcaacg cgccccaagc gcctgatcgg
     1981 ccccctcatc agccgctacg agccggggca tgcctacggc gtgcacgtgg acgatgcgct
     2041 gctcgacggc gtccgctcgg acgtggcatt cagcctgttt ttgtcggcgc cagagagcta
     2101 cgagggcggc gagctggtga tcgagacggc ggccggcgag gaggccttca agctccccgc
     2161 gggcagcctc gtcacctatc ccgccacctt tctgcatcgg gttgcccccg tcagccacgg
     2221 cagccggctg gtcgccgcgg gctgggtgcg cagttttgtg cgcgatgccg cgcatcggga
     2281 gctcctgttc gacctcgaca ccgcgcgccg gcggctgttc gaccgcgacg gcaaatcggc
     2341 cgaggccgac ctgctcgcca aatgcacggc caatttgctg cgcgagtggt gcgacgacta
     2401 ggcgccgggc cagccgagcc tcagtcgtca ccctccgcct catctgcagc gtcggcctgc
     2461 aactggccgt agttctggac cccgattttg ctcaggagat cgagctgggt ctcgaggaag
     2521 tcgatgtgcc cctcctcgtc ggccaacagc tcctcgaaca gcgccatcga cacgaggtcc
     2581 tcgttgcggc gacagatctc gcgggcgcgc gtataaagat cgcgggcatt gaactcgccg
     2641 cgcaggtcgc actccaggac ctccttgatg tcctggccga tcatcagcgg cgcgatgtgc
     2701 tgcatgttgg gcaggccttc caggaagatg atacggtcga ccaatttgtc ggcgtgctgc
     2761 atctcttcga tcgactcctt gcgctccttt ttggcgagct tggtgtagcc ccagttgtcg
     2821 agcatccggt agtgcaacca atactggtta acggcaccca attcgtgctt gagagcatcg
     2881 ttaagaatat cgatgacctc tttcttgcct ttcatgccac ctctcccgtg cctcgctcga
     2941 agggctggct cgcattgagc cgcggcgggt gaaggacgcc gggcgcacgt cagccacaat
     3001 tgcgtttcct acgccagttg gcggttacgg gccagcggcg agagtgaaag ctcaccacaa
     3061 aagcgcgggg ggtcaggcgg tttcgagcat cggccccgcg cccgcctcga gggggatttc
     3121 ggtgcgccag ggccgcggcg gggtacgctc gatcagacgc tgtgccggcg aatcgcccgc
     3181 gcaacgcagg agccggccct gggcacaggc gcaagcgtag gggcacacca cgcccttggc
     3241 cgccagcagg tcgatttttt cgtagatcgt gctcaccgcc agcggcgagc agccgcagca
     3301 cgccattttc ttctgcagat gccggtaaac gacgccgggg gtgggcaggg gtgcctcggg
     3361 caggctcagg atctcgatga gggcccgttc gatatcggcg tcactgacgg ccgcgcagga
     3421 acaaataatc atggggcgcg gctcactgtc tcgctgccgt cgcaggcgcc atcgctaaga
     3481 ggcttgcgtc ggtcgtgtag cacggcggca ggacttgttt ggccccgcgc attgtcgcag
     3541 caaagctggt cgcccccgag acgacgtcct cgatcaggga gcagccgagc agcgaaaagg
     3601 cacaggcgcg catggccgga caggcgttgt gttggcaggc cgcgatcatg gcaatagcaa
     3661 cgcattcgtc gcgacaaaag caatcgcaat ctgttgctaa ggtttcgggg cgacgttgcg
     3721 cgtggcgatc gatagagcgc acccagcgtg ccagatcgct aacggcaccc ttggccagcg
     3781 ccgggcccag gctgccggcg taggcgtccc acgccttttc ccagcaaccg atgtcgcctg
     3841 tgcgaaagcc gttgagccag tagcggaagc cgagcccaac gagacgctcg ggttcggcga
     3901 tcgcggcaat cggcagcgta tcgtccccca gcacggccgc gcccaagcgt ctcatcggca
     3961 ggctccggcc gcgcttaacc attcgagcgc agccttggcc agatacggcg gaaaaaaagc
     4021 ggcgcggcgg ctcgccgacg aattgctcat gcacgtcttc ccaattgtgg gggttcaagg
     4081 ccccgacatc ggactgcgct cgccaaggga ttgtggaggc gcgatttcaa gatttcgcac
     4141 gcggcacgct tatgtcaagc acaagcgcac tagtttagaa cgcctcgaaa tctactttag
     4201 agcggctcaa aactcggggc agcggcgagc ggacacttcg gcaactcaat tgctcgtctg
     4261 caaggagcgt cgatagcgcg aagccgccat gcccgcgccg cgcgcggcgc cggaattccg
     4321 ccgccgcgcc tgggcgctgg cgcgcggggc ggtgcgcacc atgtcggcca gcgtgtgctg
     4381 atcgagaacg tcgatgaagg cgccgagcgc atcgtccaag atctcgttga caccgctgcg
     4441 gctcctctgc cggcgcgccg gcgcgcagtc ttccaactcg acatgggtga tctcggttgc
     4501 ccgcaccacg tcgccgatgc ggatctggtc ggcccgccgc gccagccgca ccccgccgtt
     4561 gcggccgcgc acggccgcga tcagtcccgc gcgcgccaac aggttgacga tcttgaagac
     4621 gttctgctgc gtgatttgca ggcgctcgga cagctccgcg accttgatca accgatcgtc
     4681 ggcgccggcg caatcgagca aaatccggat cgcatggccg gtagatttgg tcaggcgcat
     4741 cgtcgtgaag gctccctggt ccgcgtctca acccaggcta acgcatcttg gccggaattg
     4801 cgaagccttc aggcggggcc acggccgggc cggtgggccg acctggcgtt cctcaccttt
     4861 tcttagggcc cacgccgggc ggcggggagg ccaccggggg cgtggccccg cgctacttgg
     4921 tgaggatcag cttgccgttg gcggtgatgc gcaggcgata atcgtggccg tcgtgttcga
     4981 ggatcacctc gcgctctccc tgcaacagct cgctcacctt gagacgcctg cgccggtcgc
     5041 ccgccggggc ggccgcaccc ggcggccgct gcggatcctc gtcgctgctc atggccaggc
     5101 caccccctcc aaacggcttg cccccaggct gccagctggg cctattcttg actcaataaa
     5161 tctagaaatg gcgccgttgg caagacccgt ggtcgccaag ccacaaggcg cgacgcactc
     5221 ggcggggggc cgcgccggcg ccggggcggg acggtggagg tcgcggctct tatctcccag
     5281 gcgtcgctac ttcgcgtcct gcgccttgat tttggcgtcg cggatcacct gggaccactt
     5341 ctccctttcc gtcttgatcc gctgggcgaa ctcctccggt gaactcgcca ccacctcaaa
     5401 gcccaactcg tcaaggcggg tctcaacgtc gggcagagcg acgagcgcga cgatctcccg
     5461 gtgcagcagc tcgatgaggg ccttgggtgt gccggccggg gccaggatcc ccgtgagcgt
     5521 ataggcctcc tgtccgggaa tacccgcctc ggccatggtc ggcacgtcgg gcagcgcctt
     5581 ggagcgtttc tcagccgtca cggccaggcc ccgcaacagg ccttgctgaa tttgcggcgc
     5641 cgcgggcggc accgccgaaa acgccacggg cgtgtggccg ccaacggtcg attgcatggc
     5701 cggccccgcg cccgtgaact ggaccgtggg caaatcgaga tcgttggcga ggcggaagat
     5761 ctcgccggag aggtgcggcg tgctgccgac gccgggggcc gcgaagctat acttgcccgg
     5821 gttcgccttg atgacctcga tcaattcccg catactcttg gccggcacgg agggatgtac
     5881 gctcaccaca ttgggcgata cggccacgat gctgatgggc gcaaaatcct tgaccgggtc
     5941 gtaggggact ttggcaaaca ggctggggtt gataatgaag ccggtgctga ccacggtcag
     6001 cgtgtagcca tcgggcgggg acgccgcgac catgccggaa gccgtgttgc tgccggcgcc
     6061 cggctggttc aggacgtaga actgtttgcc caacttggcc gatagcttgt cgcaaatgat
     6121 gcgggcaacg acatcggtgg gcccacccgc ggcaaacccg accaccactt tgacgggtcg
     6181 gtcgggatag gtctccgcgg cggccgtggc ggcaagcgtg ctgaggccaa gcaggatcaa
     6241 ggcaagcaat cgcacgggcg ttctccacgc gttcgttcga gggtttgcgc aacatggtag
     6301 catcgctgcc gggcgagacc agcagcctcg cgcgacccgc ccgttggctg gcaggatcgg
     6361 gcgaagacaa agtctgccgc gcagcgcgcg gcccccgcgc gccgaccaca acgacgggtt
     6421 ggcgctcaaa ccgaggcaga tcgttgcaag gtcgcggccg cgagcgccgt gggcgcctgc
     6481 cgaactgcaa gcacctttgc agcggccgtc ggcgcgggga aatcggaggc ccgctattgc
     6541 tccgaaacga cataatttga ccacctcggg ctgctgatgt tgattgatca tctctgacct
     6601 gggggtcctc atgtttacgc gtaacccgtc tgcccaaccc aacacgctcg tccaaccgag
     6661 cccaccgagc gcgcgcccgg ccaccccggc cgtggtcaat ggcgcgcagc ggcggacggg
     6721 gccgacggag agcggggaca agtcgatcat cggcaacgac ttgcgcatta tcggccaggg
     6781 attgcgcatc atcagccgca gcattctgca aatcgacggc gagatcgagg gggacgtgca
     6841 ggccgcggag gtgatcgtcg gcgagcaggg caaggtctcg ggtttggtcg ccggccagca
     6901 ggtcgtggtg agcggcaagg tgtccggtgc catttgcgcc aagtcggtca ccctgcaaag
     6961 cacctcggaa gtcgagggcg acatccatca catgtcgttt gccatcgagc agggcgccgt
     7021 attcgaggga cgcagccgac gcgccgcgac cgaggcagac ctgaatgccg tgatcgatgc
     7081 caagggtggg caatcaaggc ccgcttgagc gcctagcgat ttgtcaagcg cggcggccgc
     7141 gggcgcggcc gcgccgcggc ggcctagcgg tcgaaagcgt cgggatagtc ccgcacgctg
     7201 cgcagacagg cgacccgcca ttccttcttg gtcatgtggg tctgcgcgtc ccaggtctcg
     7261 aggcacatcg atagcttctt ttccttggat ggcgccgcct gcgtcgtatc cgcggcgatc
     7321 accgcgcgat ccttgctggt cttcatgggc gccgacggct tctgctcgct ttgtgcctgg
     7381 gccaaggaac tcgccgccaa cagcgccaag ctcgcaactc cgatgcctgc ccgagagttt
     7441 gccatggcag cctccatcgc ctcctcacga gagcgctttg ctcccagggc ccaaatatct
     7501 agaggggcag ccgggggtcc ggcataggtg aaagccctga gggggcaggc ggcggatacg
     7561 gaagaaagag gccatcccgg cgggcacctc ccccctgaat tgcgcgggct cggctgctcc
     7621 aggcgcgaag gcggcattag ttttgctcgg cttgtgcttc aaatcactgc gcaaattggc
     7681 aaaacgtccc agcatctggc gtagcgatga cggcgacaat ggtgttgagc gcatcgctcc
     7741 tgaagttgga gatgtcagcc atgaagacca tcctctcagc gttgatcgcg ctttcggttc
     7801 tcgccggctt cgcaggcatg gcggcggcgg ccgtcgatgc caagactttc tatgatcagg
     7861 tggatcgcga tcggttctga tcggccggcg ggtgacgcga tgcccctcgt gtgggctgcc
     7921 agcatgggcc ggtcatcgcg ctcggagatg ttgcgggatc cgagtttcgt cacgtcgcaa
     7981 gctgtcggag atcatgacta gcgacgcgct ggtggtcctc cgaacagcga cgaggcagca
     8041 gggagcctac ggcgtgcaag ccgcggttcc ccgaccaccg ccacaagcgc acgcagggtg
     8101 ggtgccgcca gggggcaccc tctcaagggc catctctgcg gcgcggccag gggcgcgagg
     8161 ccacatgtat ggtccacccc ttggctggcg gtccgagccc agcgcggctg gcaagccacc
     8221 acgaccccgc gctgcgctgc cggtccttga cccatgcccg ccgcaacgtt ttttgcgtac
     8281 tcgtcaaccc tcgccgagaa cgtaccatga cggttatcgt tgtgacgcag gaccgaggcg
     8341 tacgtggacc gcaactcgcg gcgcgcatcg ctgccggttt gggtcttgac ctcgtgtccc
     8401 aggagcagct cggtcatcgg gttgctgctc gcatgcacat caacgaggag acgctccgcc
     8461 ggctcatcga ggctccgccg cccctcttca cgcgctggct gggcgagcgc cggcggctcg
     8521 ccttctatac cgccgaagag gtcgccacgc tggcgcgaca gggggatgtg gtcattgaaa
     8581 gctggaagcc ggtcgcagcc gtgaccggca tcagacacgg cattcgcgtg cacatcggcg
     8641 aaccccggcg cgccgttcga ctgggaacgg gcacctcatg ctgccaggcc gccatcagcc
     8701 cgctggaagc tccgctggcg cgccgctggg ggctcgatgg cgcccaagcc gggtcgattg
     8761 tgtgcgatct cgtgctggcg cccgcgccgg aggccctcga aagctgcctc acccagctcc
     8821 gacagttcgc ctttggtctg ccgcagcaag ccccgcatgc cctcgaggag acctttgcag
     8881 ccgcaacgga cgaagcggcc gctgtctccg caccggccac ccgatggcgc cgcgcccgcg
     8941 gcacggaggt tgacatcggt tcggaacgca tgccgctcga cggcgtgacc tcgcccgagc
     9001 aggccatcgc caggatcgag gagcgcttgc acggacgacg ccggccgccg aggcggctgc
     9061 cgctgccacc tgacatgctg taggcggtgt tgagacgtca gtctctcgac ggggtcgcgg
     9121 caatttcggc caggccgtcc gcgtcggtga tgatgatctg ctgctcgttc accgttatca
     9181 cattggcgtc gcgcagccgc cccagctggc ggctgacatt ctcgcgggaa atgctgagat
     9241 agctgccgag ctcggtctgc gacatcgtga gctgcagccg taccccgttc ttgccggtgc
     9301 gaccgtgttg acgcgccagc cgcaggaggc cgcgcgccgt gcgggcgcgc atttccaagg
     9361 tgttgtcttc gatgatggcg gaggtggagc gcagcttctc gcacaggatc tggatgatct
     9421 ccaggagcgc ggcgggatgg gcggtcaagc tcggcaacag gtcacgcgcg tggaccgcaa
     9481 acatctcgca gggctccaag gcaaccgcat cggccgtgcg ttcgttacca tcgagcgcgc
     9541 cgatctcccc ggtaatgtcg ccgacgccca ggaagttcaa cacgacttcc ttgccgtcga
     9601 cattgacatt gctgaccttg atgcggcctt gcagaagaac catgacgctg tcaccggctt
     9661 ctccgcgccg gcagatgacg tcgcccctgg cgaacttttt ggtgtggcca cgcctgatca
     9721 gggcatcgag tgcggcatcg ggaagtccac cgaaaaacgt gttgttcctg agaaaaggac
     9781 ggacacggtc gataaatgac ggatcagcgc tcatggtctt cggcccttgt cgcgcaacgc
     9841 gtacactttt tttgaacttg gccgggagca gcgctgcacg acggttattg agagcagtat
     9901 gcgtcaaccg cccgtcgaac gccatgcccg tttgcggtcg gagcggtgcg ccgaccacat
     9961 tcgcgcattg cccttaatat cagctgttca gggggccgcg gcggaagcgc tgtcgcccgt
    10021 ccgcggcccg cagggcgtga gccacgggcg ttgccgcggc aggtctgccc gggcgggggt
    10081 aagcgcgatt gttgtgggtc gtctagccgg tgccgcgtcg cagcatcgaa tggccctgac
    10141 gcgggcgcct tttttgccta gagttggcaa gacggatgcc gagtgcgcac gcggatcaga
    10201 ccgtggccgt ccacgaccca aaacaacgac agcggcgcgt tcgagctccc gtctagaagg
    10261 gagaaacatg atgcgcagca ctacgcttac gcgacgtcgc ttgcttgcca ccagtgcagc
    10321 ggcgagcgcg atggttgcca tgccccacgt ccgcggcgcc catgccgcgg gcaagctcac
    10381 ggttggtctt tgggatcact gggtgcccac cgccaatgcc gccagcacgg ccctcatcga
    10441 ggagtgggcg gcaaaagaaa gggtcgaggt ccaaatcgac tacatcacga cgcaaggcaa
    10501 caagctgttg ctgaccgagc aggcggaagc ccagtcgaga acgggacatg acatcttcac
    10561 ctttcgctgc tggggcccgg ccgatcacgc caaactgctc gaaccgctcg acgatgtgat
    10621 ggagccgctg atccagcaaa acggtgccgc caacgcgacc gctcaatatc ttggtaaatc
    10681 cgagggacac tgggtggccg tgccggcaac acggggcagc aatgtcaagg gaccgtgctc
    10741 gcgcatcgat ctcttgaaga agcatgccgg catcgacgtg caggccatgt atccggccgg
    10801 cggcgaaccc aaggcggagg cctggacgtt ggacgccttc ctgcgggcgg cagaggcgtg
    10861 ccataaggcc ggcttcccgt tcggcatcgg cctcggtacc acgaccgatt ccgtcgatag
    10921 cgccggggcg atcttcgatg cctttggcgc ccagctggta gacgctcagg gcaaggtgac
    10981 ggtaaagacc gacgcggtgc gccaggcgct ggactactat acgcgccttg cgcgtttctt
    11041 tccccctgac gcaccggcct gggacgacgc ctccaacaat aagtatttga ttgccggcaa
    11101 gggctccctg atcctgaacc cgccgagcgc ctgggcggtg gccaagcgcg atgccccgca
    11161 ggttgccgaa cagctatgga cgcatggcat gccggcggga ccaaaaggcc gcttcacccc
    11221 gtgcattccc tatttctggg gggtctggtc gttcggcaag aacaaatcgg cggccaagaa
    11281 cctgttgacc ttcctgtcac agccgcccgc ggtcgagaag atggtggcag ccagccaagg
    11341 cttcgatatt cccgcctttg ccaatttgac ccaactcaag acctgggccg aggagggacc
    11401 acccaagggt acgctttatc actatcccaa ccgcaacgag catcagatcg tgtcgatcgc
    11461 cggggcaccc gccccgccca agattgccgt gcaaatctac gtgcaaggca tcatgaccaa
    11521 gatgaccgtg cggcatttgc agggcgagcc gatggaaaag gtgctggcct gggcggagac
    11581 cgaggtcgag ggctttttgc gcacgtaaag gtggcgactc gaggcgagga cggcgccgcg
    11641 ccagctcggg cgcggccgct tcgcttcggc cccgcggctc ggccatctca tagcgtcggc
    11701 gatgacgtgg gggacggggg aggtgccggg atgggtgcgc gcccctgggg tcctatcgag
    11761 atcggcttgc tccttactta gctattcagt
//
LOCUS       DASTMT010000196         1890 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_434391, whole genome shotgun sequence.
ACCESSION   DASTMT010000196 DASTMT010000000
VERSION     DASTMT010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 1890)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1890)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1890
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_434391"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..475)
                     /gene="dctP"
                     /locus_tag="VFR00_04500"
     CDS             complement(<1..475)
                     /gene="dctP"
                     /locus_tag="VFR00_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019404830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HEU0158547.1"
                     /translation="MRFATYLLLAALLSLAGAPGAGAQTKLKWAHVYETAEPYHSEAL
                     WAAEEIKKRTGGRYQIDVFPASQLGNENQINEGLGLGTVDMIYTGVAFAGSIHKPIAI
                     TNAPYVLRDFEHWKLYRDSALFREIAKGYEDKTKHKVMSLTYYGQRMVTANKEIKK"
     gene            complement(570..1274)
                     /locus_tag="VFR00_04505"
     CDS             complement(570..1274)
                     /locus_tag="VFR00_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HEU0158548.1"
                     /translation="MPSTRRHRHFDLLMVGFVVVLVCSNLIGPAKIAVVDLPLWGPFV
                     FGAAAIFFPISYVFGDILTEVYGYAEARKVIWVGFAGLVFASAMAAIVVALPPAPFWP
                     HQQAFEIAFGNTWRIVAASIIAYFCGEFVNAYVLAKMKVMTGGRVLWTRTIGSTIAGE
                     AVDSALFYPLAFYGTGIIPDAALPAVMLAQFATKTLVEVIFTPVTYAAVGYLKRAEGV
                     DHFDRDTDFNPFKIGG"
     gene            1439..1600
                     /locus_tag="VFR00_04510"
                     /pseudo
     CDS             1439..1600
                     /locus_tag="VFR00_04510"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            1706..>1890
                     /locus_tag="VFR00_04515"
     CDS             1706..>1890
                     /locus_tag="VFR00_04515"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006020705.1"
                     /note="NADPH-dependent; catalyzes the reduction of
                     7-cyano-7-deazaguanine to 7-aminomethyl-7-deazaguanine in
                     queuosine biosynthesis; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HEU0158549.1"
                     /translation="MAGAGLTALGQKAPTPLSPATALLERVANPHPDTRYVARFTAPE
                     FTTLCPVTGQPDFAHLV"
BASE COUNT          365 a          577 c          615 g          333 t
ORIGIN      
        1 gcttcttgat ctccttgttg gcggtcacca tgcgctggcc gtagtaggtg agcgacatca
       61 ccttgtgctt ggttttgtcc tcatagccct tggcgatctc gcgaaacagc gcggagtcgc
      121 gataaagctt ccagtgttcg aaatcgcgca acacgtaggg cgcgttggtg atggcaatcg
      181 gcttgtggat ggagccggca aaagcgacgc cggtgtagat catatcgacg gtgccgagac
      241 ccaagccttc gttgatctgg ttctcgttgc cgagctggga ggcgggaaag acgtcgatct
      301 ggtagcggcc gcccgtgcgc ttcttgattt cttcggctgc ccacagcgct tcgctgtgat
      361 aaggctcggc cgtctcgtag acgtgggccc atttgagctt ggtttgggcg cctgcccccg
      421 gggcaccggc aaggctcagc agcgccgcca acagcagata ggtggcaaat ctcatgtcgg
      481 ttttcctccg tcccgcaaag ctcaacgccc aacgcgaagt tatcgtataa gcagacgact
      541 tggcgtgcta ggcgattttt cgcgggactt caaccgccga tcttgaaggg attgaagtcg
      601 gtatcgcggt cgaaatggtc gacgccttcg gcgcgcttca aatagccgac ggcggcgtag
      661 gtgaccggcg taaagatcac ctccaccaag gtcttggtgg cgaattgcgc cagcatcacc
      721 gccggcaagg ccgcatcggg gatgatgccg gtgccataaa aagccaacgg ataaaagagt
      781 gcgctatcga cggcctctcc ggcgatggtg gagccgatgg tgcgcgtcca caacacgcgc
      841 ccgccggtca tcactttcat ttttgccagc acgtaggcgt tgacgaactc gccgcagaaa
      901 taggcaatga tggaggcggc gacgatgcgc caggtgttgc cgaaggcgat ctcgaaggcc
      961 tgctggtgcg gccagaatgg cgccggcggc agcgccacga cgatggccgc catcgccgag
     1021 gcaaacacca ggccggcaaa gcccacccag atcaccttgc gcgcctcggc gtaaccgtag
     1081 acctcggtta ggatgtcgcc aaacacgtag gagatgggaa agaagatggc ggccgccccg
     1141 aagacgaagg ggccccacag cggcagatcg acgacggcaa tcttggccgg gccgatcagg
     1201 ttggaacaca ccagcaccac gacgaagccc accatcaaga ggtcgaagtg gcggtgacgg
     1261 cgagtgctgg gcatcggcgg cttccttggc tggcgcgtgg gacgcctgct tagcaaagcg
     1321 cacaccgttg tctaagtaga attgtcggtc tagtctcaag tctagtatta caatacaatt
     1381 agccatgccg tcgggcctgg ggcaacccct cggcagccgc ggcgcggaag gaagagccat
     1441 gatcgccagc cttcgcccgg agctcgcgcc cttgggcaag cgcatacagc aggcgcgccg
     1501 gggcgcgggg ctgacccagc cgcaggtggg ccgcgccttg ggcgtgagca agcagcaggt
     1561 cagccattgg gaaacggggc gctccgacat cacgcgcgag cacttgctgc acctcgccaa
     1621 gctgttcgac gtcgagatgg attggctggc gcgcggcacc ctgatcgagg tcgccgatgc
     1681 tgcaggcaac ggcgccgggg cgcaggtggc gggtgccggc ctgaccgcgc ttggccaaaa
     1741 ggcgccaacc ccgctgtcgc cggcaacggc attgctggag cgcgtcgcca atccccaccc
     1801 cgacactcgt tatgtggcgc gcttcacggc cccggagttc accaccctgt gtcccgtgac
     1861 cggccagccc gacttcgcgc atctggtgat
//
LOCUS       DASTMT010000197         7215 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_2399384, whole genome shotgun sequence.
ACCESSION   DASTMT010000197 DASTMT010000000
VERSION     DASTMT010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 7215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7215
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2399384"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            197..643
                     /locus_tag="VFR00_04520"
     CDS             197..643
                     /locus_tag="VFR00_04520"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEU0158550.1"
                     /translation="MTIPQHREVAAALIIDQRGRFLLQQRDDVPGILFPGKIGLFGGH
                     REGNESFLECVVREVHEEISFFIPPERFEHLASYRGSDPYIAGGTLWRELYLTTDVPV
                     EPLRITEGSLLIAEMADIAALVPRFAPSAHAAIHEYLTRTGRAQNQ"
     gene            complement(665..1285)
                     /locus_tag="VFR00_04525"
     CDS             complement(665..1285)
                     /locus_tag="VFR00_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012331205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEU0158551.1"
                     /translation="MIKQLALLAVLSLEGTSVMAQDRMPEIPADRMTEAQKKAASEFA
                     EGRGYAVRGPFAAMLRSPEVMLRAKAMGDYVRFKSTIPARLNELAIIITARQWSQNYE
                     WQAHRPLAEKAGLRSEIAQAIADGRRPTGMSADEEIVYDFCMELHANKSVSDATYQRA
                     VEKFGEQGIIDLTAANAYYSFNAMMLNVARTALPAGAKAELQPFPK"
     gene            complement(1424..3094)
                     /locus_tag="VFR00_04530"
     CDS             complement(1424..3094)
                     /locus_tag="VFR00_04530"
                     /inference="COORDINATES: protein motif:HMM:NF014585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HEU0158552.1"
                     /translation="MHSQPISPIALSVIWNGFLSIAEEMGTTLQMTAFSEAVREGQDF
                     STAIFDSRGRLVAQGNFTPGHLGSMPYVIKSIMEIFPPGALKPGDGVLLNDSKLGSGH
                     YPDFFFVSPVFLESTLIGYLINTAHHIDVGGAAPGSQKVQGISERFQEGLRILPVRVL
                     RGGEFDEDILRLILGNVRLPEILRGDLTAQRNANFVGAQRLAKLYQEHGPELIARAID
                     EILDRSEARMRELIRAIPDGRYSAEDWLDACGEECGPIKVAVDIDVAGDEITVDFSRS
                     SDQVPVALNSYINYTRAYTLFSIKVFTDALLPQNGGMIRPIHVKAREGSFFNPREPAP
                     SGGRAIIQIRIFEVINGALAQALPDKAFSAFSHWSNPNIGGLDPRTDKPFVMYDLMFG
                     GYGARAGHDGAEALSPVVNCANIPVEVHETINPVLVRRFELIADSGGAGRWRGGCGIR
                     KDIELKAETAVMTLLGERHSHPGSALAGGEPGSLASTTVLSGNERVEMGSKDVRALKR
                     GDVVSFRLNGGAGWGQARERDPDRVADDVAEGYVSAEAAKRVYGWRGA"
     gene            complement(3105..5177)
                     /locus_tag="VFR00_04535"
     CDS             complement(3105..5177)
                     /locus_tag="VFR00_04535"
                     /inference="COORDINATES: protein motif:HMM:NF014071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HEU0158553.1"
                     /translation="MMRVAFDIGGTFTDFVLEDVPAGRLAFHKVPTTPSDPAAAVLAG
                     LDALCKDANLAPDQIGGILHATTVATNAILERKGPKTALITTAGFRDILIIGRQKRYD
                     TYDMYLAKPVPLLRRRDIYEVAERTLADGSVEAPLDLAELERVLDRLETQDYASVAVC
                     LLHAYARGEHERLIGERLAQRRPERAISLSSEVSPKFREYERASTVVANAYVKPIVNG
                     YIRRLTGALAQKGIVSDLFIMQSNGGLVPPELACEMPIRIVESGPAAGVLLCGVIGAE
                     EGLERVLTFDMGGTTAKLGAIDGGEPAISPTFEVDQVRYRRGSGLPINVPALELLEIG
                     AGGGSMARADMGLIKVGPESAGADPGPACYRRGGTQATVTDANVVLGYINPGNFNGGA
                     MRLDAEAAAEAVQRELGAPLGLDTGEAAWGIYTMANANMERAMRIVSVERGRDPRRYG
                     LVAFGGAGPLHAGRLARALGIPKIIVPWGAGVGSAIGLLEANTKLDQSLTHLIALRAG
                     AEAEIAEIYRALEARINADLRRLASTLPPVMARFAFLRYLGQGHEIRVDLPAFPSGEN
                     YITELVARFEAAYLAKYGYRQAGAAVEAVDWYLVATIPNGAAVADRARGWRGEGGGEA
                     RQGARKAYFPELGGYVDTPVFERRALGVGEVVSGPAIIEEAEATTVVLPKAQAVVSGR
                     GHLVIDVG"
     gene            5362..5568
                     /locus_tag="VFR00_04540"
     CDS             5362..5568
                     /locus_tag="VFR00_04540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0158554.1"
                     /translation="MRKAELVEMAAAAGLPRLSDKHLDQLENSAASARELAQKLPKDL
                     HWSEELSLTFRLPRPTGEEPKCAP"
     gene            5565..6959
                     /locus_tag="VFR00_04545"
     CDS             5565..6959
                     /locus_tag="VFR00_04545"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEU0158555.1"
                     /translation="MSDLAYLSITEASRRMRTGRLSPISYTEALLDRIQRLNERFHAF
                     IRTTPDLAMAAARQAEREIAAGNWRGPLHGVPFALKDIIDWSGLPTTAHSKLLADNIA
                     ASDAVVSGRLKAAGAIMMGKLATHEFAIGGPSFDLPWPPAVNPWGGKHFPGGSSSGSG
                     VALAAGLIPAALGTDTGGSVRNPASMCAIVGMKPTYGLVSRRGVFPLANSLDHVGPMT
                     RNVRDNALLLQVLAGHDGDDPGSAEVPIPDYAAGLEEGVKGVRIGLVRHFYADDCHAH
                     PEQIAALEQAAEVLRSLGAGVQEVRLAPLDEYATATRIIIRCEAFAIHRQWLATRPED
                     YGDLARQRILDGAVVSAADYIDALRLRARLTRQTLAAFADIDVALTASNMDPTCPIDD
                     AEACARLYPRQARQPFNITGQPALAMPAGFSRDGLPLSLQLIGHPFQEAMVYRVAEAY
                     ERATAWTERHPPGL"
BASE COUNT         1225 a         2445 c         2323 g         1222 t
ORIGIN      
        1 agcgcgacaa ggcggcgcgt ctaacccccc acgccgccat tgccaccgca cggctatttg
       61 cggggcccgg ccgccggacc cgtgcgcgcg ttgagcacct tgaatccggt tttcgctgca
      121 gatcgggatt gaccgatgcc ctgaggtttc gaacactcgc ctcaggataa tgcgaccttg
      181 ccatgagagc tttgcgatga cgataccgca gcaccgagaa gtagctgccg cgctgatcat
      241 tgatcaacgc gggcgatttc tgctgcagca gcgcgacgac gttcctggca tcctttttcc
      301 cggcaagatc ggcctgtttg gcggtcatcg cgaaggcaac gagagcttcc ttgaatgcgt
      361 ggtcagggag gtgcatgaag aaatcagctt cttcataccg cctgagcgtt ttgaacacct
      421 cgcgagctac cgagggtccg acccctatat tgccggcggc accctttggc gtgagctcta
      481 cctcaccacg gatgtgccgg tagaaccctt gcggatcacc gagggaagcc tccttattgc
      541 cgagatggcc gatatagcgg cgctcgtgcc gcggttcgca ccgtcggcgc acgctgctat
      601 ccacgagtat ctgacccgca ccggcagggc acaaaaccaa tagcgatcgg caatccccct
      661 ttcgctattt gggaaacggc tgcagctccg ctttggcacc agcgggcagc gcggtgcgtg
      721 ccacgttcaa catcatggcg ttgaagctgt aataagcatt cgcggcggtc aggtcgataa
      781 tgccctgctc accaaacttt tctactgcgc gctggtaggt cgcatcgctc acgctcttgt
      841 tggcgtgcag ctccatgcaa aagtcgtaga cgatctcctc gtccgcgctc atgccggtgg
      901 ggcggcggcc gtcggcaatc gcttgcgcga tctcggacct caggcccgcc ttttccgcca
      961 gcggccgatg cgcctgccat tcgtagttct gcgaccattg gcgcgccgtg atgatgatgg
     1021 caagttcgtt cagtcgtgcg ggtattgtcg atttgaagcg cacataatcg cccatcgcct
     1081 ttgcccgcag catgacctcg gggctgcgca gcatcgcggc gaacggtccg cgaaccgcat
     1141 aaccgcgccc ctcggcgaac tcggacgccg ctttcttctg cgcctcggtc atccggtcgg
     1201 ccgggatttc gggcatgcgg tcttgagcca tgacagaggt tccttccaac gatagcactg
     1261 ccagaagcgc gagttgtttg atcatcggcg atccttcctc tgatgtcgag cgatgatcca
     1321 atgcatcgcc ctgcgcagca gcggcgatcg acgcgcgagc ccattgcgca aagtgcgtgc
     1381 cgacatccga ccggtgaagc ccctggcaag cccgcaaaac catctatgcg ccgcgccagc
     1441 catagacgcg cttggccgct tccgccgaca cgtagccttc ggccacgtcg tccgccaccc
     1501 gatcggggtc gcgctcgcgc gcttgccccc agcccgcgcc accgttaagc cgaaagctca
     1561 cgacgtcgcc acgcttgagc gcgcgcacgt ccttggagcc catctcgacg cgctcgttgc
     1621 cgctcagcac cgtggtcgag gcaaggctac ccggctcgcc gcccgccagc gcggaaccgg
     1681 gatgggaatg ccgctcgccc agcagggtca tcaccgccgt ctcggccttg agttcgatgt
     1741 ccttgcgaat gccgcagccg ccgcgccagc ggcccgcacc gccgctgtcg gcgatcagct
     1801 cgaagcggcg caccagcacg ggattgatgg tctcgtgcac ttcgacggga atattggcgc
     1861 agttgaccac ggggctcagc gcctcggccc catcgtggcc ggcgcgcgct ccatagccac
     1921 cgaacatcag atcgtacatg acgaacggtt tgtcggtccg gggatcgagg ccgccaatgt
     1981 ttggattgct ccagtgcgag aaggccgaga acgccttgtc ggggagggcc tgcgccagcg
     2041 ccccattgat cacctcgaaa atgcggatct gaatgatggc gcggccgccg ctgggcgcgg
     2101 gttcgcgcgg attgaagaac gagccttcgc gcgccttgac gtggatcggc cggatcatgc
     2161 cgccgttttg cggcagcagc gcatcggtga agaccttgat ggaaaacagc gtgtaggcgc
     2221 gcgtgtagtt aatgtaggaa ttgagcgcga ccggcacctg atcggaggaa cgcgagaagt
     2281 cgacggtaat ttcgtcgccg gcaacgtcga tatcgaccgc gaccttgatg ggaccgcact
     2341 cctcaccgca agcgtcgagc cagtcttcgg cgctgtagcg cccgtcggga atggcccgga
     2401 tcagctcgcg catgcgcgcc tcggatcgat cgaggatctc atcgatcgcc cgcgcgatca
     2461 gctctggccc atgctcctga tagagtttgg ccaggcgttg cgcccccaca aagttggcgt
     2521 tgcgctgagc ggtgaggtcg ccgcgcagga tctcgggcag acgcacgttg ccgaggatga
     2581 gacgcaagat gtcctcgtcg aactcgccgc cacgcagcac gcgcacgggc aggatgcgca
     2641 ggccctcctg gaagcgctcg gaaatgcctt gcaccttctg cgacccgggc gccgcgccgc
     2701 caacatcgat gtggtgcgcc gtattgatca aatatccgat tagcgtagac tcgagaaaaa
     2761 cggggctcac aaaaaagaag tcggggtagt ggcccgagcc aagcttagag tcgttgagga
     2821 gcacgccgtc cccgggcttg agcgcgccgg gcgggaagat ctccatgatc gacttgatga
     2881 cgtagggcat cgagccgagg tggccgggcg tgaagttgcc ctgcgccacc agccgcccgc
     2941 gggaatcgaa aatcgccgtc gagaaatcct gcccctcgcg taccgcctcg gagaaggccg
     3001 tcatctgcaa ggtggtgccc atctcctcgg caatcgacag gaagccgttc cagatgaccg
     3061 acagcgcgat cggactgatg ggctgactgt gcatgcctta tcccctagcc aacgtcgatc
     3121 acgaggtgcc cgcgcccact gacgacggct tgagccttcg gcagcacgac cgtcgttgct
     3181 tcggcctctt cgatgatcgc ggggccgctg acgacctcgc cgacgccaag cgcccgccgc
     3241 tcgaacacgg gtgtgtcgac gtaaccgccg agttccggaa aataggcttt acgcgcaccc
     3301 tggcgcgcct cgccgccgcc ctcgccgcgc cagccgcggg cgcggtcggc caccgcggcc
     3361 ccgtttggga tcgtggccac cagataccaa tcgacggcct cgaccgcggc cccggcttgc
     3421 cgatagccgt acttggcaag gtaggccgct tcgaagcgcg ccaccagttc ggtgatataa
     3481 ttttcgccgc tgggaaaggc cggcaagtcg acgcggatct cgtggccctg acccagatag
     3541 cgcagaaagg caaagcgcgc catgacgggg ggcagcgtgc tcgcaaggcg cctcagatcg
     3601 gcgttgatgc gcgcctcgag cgcgcgatag atctcggcga tctcggcctc ggcgccggcg
     3661 cgcaaggcga tgaggtgcgt cagcgattga tcgagcttgg tattggcttc gagcagaccg
     3721 atggcggagc cgacgcccgc gccccacggc acgatgatct tggggatgcc cagggcgcgg
     3781 gcgagccggc ccgcatgcaa tggaccagcg ccgccgaagg ccacgaggcc gtagcggcgc
     3841 ggatcgcgcc cacgttccac cgacacgatg cgcatggcgc gttccatgtt ggcgttggcc
     3901 atggtgtaaa tgccccaggc cgcctcgccg gtatcgagcc ccaggggcgc cccgagctcg
     3961 cgctgaaccg cctcggcggc ggcctcggcg tcgaggcgca tggcgccgcc gttgaaattg
     4021 ccggggttga tgtagcccag cacgacgttg gcgtcggtga ccgtggcctg cgttccgccg
     4081 cgccggtagc aggcgggccc cggatcggcg ccggcacttt cgggcccaac cttgatgaga
     4141 cccatgtctg cccgcgccat gctgccgccg ccggcgccga tttctaggag ttcgagcgcc
     4201 ggcacattga tgggcaggcc gctgcctctg cggtagcgca cctgatcgac ctcgaaggtg
     4261 ggcgaaatag cgggctcccc gccgtcaatg gcgccaagct tggcggtggt cccgcccatg
     4321 tcgaaggtga gcacgcgctc caaaccctcc tccgcgccga tcaccccgca cagcaagacg
     4381 ccggcggcgg gccccgattc gacgatgcgg atcggcatct cgcaggcgag ctccggcggc
     4441 accaggccac cgttcgattg catgatgaag aggtctgaga cgatgccctt ttgcgccaag
     4501 gcgccggtca ggcggcggat gtagccattg acgattggct tgacgtaggc attggcgacc
     4561 accgtgctgg cgcgctcgta ttcgcggaac ttcggcgaga cctccgagga cagcgagatg
     4621 gcgcgctcgg ggcggcgctg cgccaagcgc tcgccaatca gccgctcgtg ctcgcccctg
     4681 gcgtaggcat gcagcaggca caccgcgacg gaggcgtagt cttgcgtctc cagccgatcg
     4741 agcacccgct cgagctcggc cagatcgagt ggcgcctcga cgctgccgtc ggcaagcgtg
     4801 cgctcggcga cctcgtagat gtcgcgccga cgcagcagcg gcacgggctt ggccagatac
     4861 atgtcatagg tgtcgtagcg cttctgacgg ccgataatca ggatgtcgcg gaagccggcg
     4921 gtggtaatga gcgcggtttt tggacctttg cgctccagga tggcgttggt ggcgaccgtg
     4981 gtggcgtgca ggatgccgcc gatctggtcg ggggcgaggt tggcgtcctt gcagagagca
     5041 tcgagacccg ccagcacggc ggctgccgga tcgctgggcg tggtcggcac cttgtggaag
     5101 gcgaggcgac cggcaggtac atcttccagg acgaagtcgg tgaacgtccc gccaatatcg
     5161 aaggcaacgc gcatcatcgt gtgcggcatt tcctttattt gcgcgcaacc ctggaggtca
     5221 aaaggctagc ccgagcctct ccccgcaagc aaccgccaaa gccgcggggc agaattccac
     5281 ttggcggccg accctttccc caaggcgaca gttgaggcaa agtttccacg cccgttcgca
     5341 accggcatgg aagggagcca cgtgaggaaa gccgaactcg tcgagatggc cgcggcggcc
     5401 ggcctgccgc ggctcagcga taagcacctg gaccagctgg aaaatagcgc ggcctcggcg
     5461 cgcgagcttg cccaaaagct gccaaaggac ctgcactggt ccgaggagct gagcctcacc
     5521 tttcgcctgc cccggcccac gggtgaggag ccaaagtgcg cgccatgagc gatcttgcct
     5581 atctttccat cacggaagcg tcccggcgca tgcgcaccgg caggctctcc cccatcagct
     5641 ataccgaggc cctgctcgat cggattcagc gcttgaatga gcgctttcac gccttcattc
     5701 gcaccacgcc cgatctcgcc atggcggccg cgcgacaagc cgaacgggag atcgccgccg
     5761 gcaactggcg cgggcccctg catggggtcc cctttgccct caaggacatt atcgactggt
     5821 ccggtttgcc gaccaccgcg cactcgaaac tcctcgccga caacattgcg gccagcgacg
     5881 ccgtggtaag cggacgtctc aaggccgccg gcgccatcat gatgggcaag cttgcaaccc
     5941 acgagtttgc catcggcggg ccgtccttcg acctgccctg gccaccggcc gtcaacccct
     6001 ggggcggcaa gcactttccc ggcggctcct cgagcggctc gggcgtggcg ctggccgcag
     6061 ggctcatacc cgccgcgctc ggcaccgata ccggcggttc cgtgcgcaat cccgcctcga
     6121 tgtgcgccat cgttggcatg aagccgacct atgggttggt cagccgccgc ggcgttttcc
     6181 ccttggcgaa ctcgctcgat cacgtcgggc cgatgacacg caacgtccgc gacaatgcgc
     6241 tcctgttgca ggtgctcgcc ggccacgacg gcgatgatcc cggcagcgcc gaggtcccca
     6301 tccccgatta cgccgccggg ctcgaggagg gcgtcaaagg tgtgcgcatc ggcctcgtcc
     6361 gtcacttcta tgccgacgac tgccatgccc atcccgagca gatcgcggcc ctcgagcagg
     6421 cggcggaggt tttgcgctca cttggcgcag gcgtgcagga ggtgaggctg gcgccactcg
     6481 atgagtatgc cacggcaacg cgcatcatca ttcgctgcga ggcgtttgcc atccaccgcc
     6541 agtggctggc cacgcgcccc gaggattacg gcgacctcgc ccgccagcgc attctcgacg
     6601 gcgccgtggt aagcgcggcc gactacatcg atgccttgcg cctgcgcgcc cgtctgacgc
     6661 gccagacgct ggcggccttt gccgacatcg acgtggcctt gaccgcttcc aatatggatc
     6721 cgacctgtcc gatcgacgac gcggaggcct gcgcccgcct ctatccgcgc caggcccgcc
     6781 agcccttcaa catcacggga cagccggcgc tcgccatgcc cgcgggcttc agccgcgatg
     6841 ggctgccgct gtcgctgcag ctgatcggcc atcccttcca ggaggccatg gtctaccgcg
     6901 tggccgaggc ctacgaacgt gccacggcct ggaccgagcg ccacccgccc gggctataag
     6961 ggatggacgc gtgcctggca ggcaaggaaa agccccacct gggggacaca gccggtccgc
     7021 cggcagggct cggggcgggc gcgcgccaaa tctcggacgc ccaacaggga gtgccgtcat
     7081 gcacaccaga ctacgcggcc tacgtcttgc cgccctcgcc ctcgcgctca gcgccgccgc
     7141 cgcgcccttg gcggcggccg agatcaaggt tttgacggcg ggcgccatgc gcggcgtgct
     7201 cgaggcgctg ctgcc
//
LOCUS       DASTMT010000198         5478 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_1416106, whole genome shotgun sequence.
ACCESSION   DASTMT010000198 DASTMT010000000
VERSION     DASTMT010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 5478)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5478)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5478
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1416106"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            34..1110
                     /locus_tag="VFR00_04550"
     CDS             34..1110
                     /locus_tag="VFR00_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0158556.1"
                     /translation="MRTRNAAAARREQLGRTLTVLATLAALGLTGCESATSLFGSSAT
                     PPQDSIVAAPVAPQPPLAKVVVAPIIGAPDAVAKQIQQEFTSAIEKQRVAVAASKDER
                     VDYTLRGYIVAAKDKQGTKVSYIWDVTDPTGKRVNRVTGEEMASAGGAKDPWAALTPA
                     VAQSIAGKAANSFATWLTSRGQSAVASAAPHPAGVGAQATEGAEAKAPAPTKSAVAAA
                     TPTAAPAASGGTTTGSIGNEGPLAAVVPNVNGAPGDGSTALASALQRELSGKGITVGN
                     KSASAAYRVEGTVTMGAAKEGKQPIQIEWLVRDPQGKKLGTVSQKNEIPEGSLDGPWG
                     KTAEQAAGAAAQGILKLLPQKAVN"
     gene            1272..2204
                     /locus_tag="VFR00_04555"
     CDS             1272..2204
                     /locus_tag="VFR00_04555"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HEU0158557.1"
                     /translation="MKLVAGNSNRPLAEAIAGALKLPLCRTVVRRFADMEVFVEVQEN
                     VRGQDIYVLQSTSFPANDNLMELLILVDALKRASAQRITAVMPYFGYARQDRKPGPRT
                     PISAKLVANLIERAGVDRVMTVDLHAGQVQGFFDIPTDNLFAAPVMVRDIQERYGTSN
                     LVVVSPDVGGVVRARGLAKRINVPIAICDKRRERAGESEVMNVIGEVKGKRAILIDDI
                     VDSGGTLVNAANALLEQGATEVLAYVTHGVLSGGAVAKITGSRLKTFVITDTIQPTEA
                     VKAAANIRVLPMAPLLAEAIARTAREESVSSLFY"
     gene            complement(2198..3589)
                     /locus_tag="VFR00_04560"
     CDS             complement(2198..3589)
                     /locus_tag="VFR00_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011666243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEU0158558.1"
                     /translation="MGSASLRTRHASGNALLQAPLLPTLLRLALPNLIALSVTAAVAI
                     AETSYVGMLGTAPLAAIALVFPMVMLMQMLSAGAMGGGVSSAVSRALGAGDEPRARAL
                     ALHALAIGLGTGLFFSLLFVLFGRELVAALGGRGAVLEQAMAYANVALSAAVLLWLLN
                     TLASIVRGTGNMQVPSLTLLGVSALQIGLGGALGLGLGPIPRFGLPGVAAGLVISCAT
                     GTLVLIWYLRSGRGRLLLRFAGIAWRREMFLDILRVGALAAFSPLQSVLTVLILTRAI
                     ASFGTEALAGYGIGARLEFLLVPIAFAVGVACVPMVGMAVGAGRIGRARRVAWTGAAV
                     SAVALGLIGVLVATLPQLWSGLFSADAEVAAAANLYLRFAGPTFPLFGLGLCLYFASQ
                     GAGRVLGPVLASSLRLGIIAIGTGVLAWSGAPLWCLFALVGISLAAYGSATAAAVYRT
                     TWGPEGPPSGGAQ"
     gene            3727..4041
                     /locus_tag="VFR00_04565"
     CDS             3727..4041
                     /locus_tag="VFR00_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008963752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU0158559.1"
                     /translation="MAARYGQVCPVARALDVLGERWSLLILRDLKRKGPLRFQELEAG
                     LQGAAPNTLSARLKALEAHGVIATRLYARHPPRYEYFLTPKGQDLAPVLKALYAWGER
                     HA"
     gene            4293..4985
                     /locus_tag="VFR00_04570"
     CDS             4293..4985
                     /locus_tag="VFR00_04570"
                     /inference="COORDINATES: protein motif:HMM:NF022515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2865 domain-containing protein"
                     /protein_id="HEU0158560.1"
                     /translation="MRIAGSVGCAAILLLGFSGAAISVRIDGSKDAPAAQPRDEGAAP
                     PAGAGSAQPPQRIANADWADRLRSEDFWQHMRSPQSRQDDASRRAPGPTRLEGRHRYA
                     PVERRDELRSKAATFASGSYRTVCVRLCDGYFWPLSFAASRASLAADRRKCEESCEAP
                     ARLYLAKDAETPLEEMRDENGRYYRDLKTAFIYRTSYVESCKCRAHPWEAAALARHAG
                     YAQSSKPPQPRR"
     gene            complement(5042..>5478)
                     /locus_tag="VFR00_04575"
     CDS             complement(5042..>5478)
                     /locus_tag="VFR00_04575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0158561.1"
                     /translation="GMAVSAADPSGLFGILKDSMATGRTLLQAKADPSSNELIKAVVS
                     DFESGDGRQAARKQVQSRLKGIAVGEMKNKSIGALRDVAQLIDSKAPGDAAGFKTWLH
                     HIAENAAEASKEGGFMGFGGTPVSDKEKATLAELASALGLAA"
BASE COUNT         1023 a         1842 c         1804 g          809 t
ORIGIN      
        1 agcagggatc atcggtgaag gggggacttc gctgtgagaa ccagaaacgc tgctgcggcg
       61 cggcgtgagc agcttgggcg cacgctcacc gtcctcgcca cgctggcggc attgggccta
      121 acggggtgcg agagcgccac gtcgctgttc ggctcgagcg cgacgccacc gcaagattcc
      181 atcgtggccg cgcccgtggc accgcagccg ccgctcgcca aggtggtggt ggcgccgatc
      241 atcggtgcac ccgacgccgt cgccaagcag atccagcaag aattcaccag cgccatcgaa
      301 aagcagcgcg tcgccgtggc ggccagcaaa gacgagcgcg tcgactatac gctgcgcggc
      361 tatatcgtcg ctgccaagga caagcagggc acgaaggtct cctacatttg ggatgtgacc
      421 gatccgaccg gcaagcgcgt caaccgcgtc accggcgaag agatggcctc ggccggtggc
      481 gccaaggacc cgtgggcggc tctcaccccg gccgttgcgc aatccatcgc cggcaaggcc
      541 gccaattcct tcgccacctg gctcaccagc cgcggccagt cggcggtcgc cagcgcggcc
      601 ccgcatcccg ccggcgtggg tgcgcaggcc accgagggcg cggaagccaa ggcaccggcg
      661 cccaccaaaa gcgcggtagc cgccgctacg cccaccgccg cgccagccgc ctcaggcggc
      721 acaaccacgg gcagcatcgg caacgaaggc ccgctggccg ccgtcgtacc gaacgtcaac
      781 ggtgcgcccg gtgacggcag cacggcgctc gcgagcgccc tgcagcgcga gctgtcgggc
      841 aagggaatca cggtgggaaa taagtcggcc tcggccgcct atcgggtgga aggcacggtg
      901 acgatggggg ccgccaagga aggcaagcag cctatccaaa tcgaatggct ggtgcgcgat
      961 ccgcagggca agaagctcgg caccgtctca caaaaaaacg agatccccga ggggtctctg
     1021 gatggtcctt ggggtaagac ggctgaacaa gccgccggcg ccgcggcgca gggaattctc
     1081 aaactcttgc cgcagaaagc ggttaactga caccaaggct ggcgctgtga ccggtcccat
     1141 gggcgggttt tacacccgcc catgccgttt acagggcccc gatgtccttg ctagaaggcc
     1201 tcggcagaac ggagcgcgcg tagatgccct tcgatacccg agacgcaaac cgtggccggg
     1261 gggcgctgaa gatgaagctg gtcgcaggga acagcaatcg ccccttagcg gaggccatcg
     1321 ccggggcatt gaagctgccg ctttgccgca ccgtggtgcg ccgtttcgcg gacatggagg
     1381 tgttcgtcga ggtgcaggag aacgtgcgcg ggcaggatat ctacgtccta caatcgacct
     1441 cgtttccagc caacgacaat ctgatggaat tgttgatcct cgtggatgcc ctgaagcggg
     1501 cttccgcgca gcgcatcacc gccgtcatgc cctatttcgg ctacgcacgg caggaccgaa
     1561 agcccggtcc gcgcacgccg atctcggcaa agctcgttgc caatctgatc gagcgcgccg
     1621 gcgtggaccg cgtcatgacg gtcgatctgc acgccgggca ggtgcaaggc ttttttgaca
     1681 tcccgaccga caacctgttt gccgcccccg tgatggtccg cgacatccag gagcgctacg
     1741 gaacctcgaa cttggtggtg gtgtctcccg acgtgggcgg ggtggtgcgc gcccgtggcc
     1801 ttgccaagcg catcaacgtg ccgatcgcca tttgcgacaa acgtcgagag cgggccggcg
     1861 agtcggaagt catgaacgtg atcggcgagg tcaaaggcaa gcgcgcaatt ttgatcgacg
     1921 acatcgtcga ttcgggcggc acactcgtca acgctgccaa tgcgctactg gagcagggag
     1981 ccaccgaggt gttggcctac gtcacgcacg gcgtgttgtc gggcggcgcg gtcgccaaga
     2041 tcacgggctc gcggctcaag acctttgtca tcaccgacac gatccaaccg accgaagccg
     2101 tcaaggccgc cgccaacatc cgcgtgcttc cgatggcccc cttgttggcg gaagccatcg
     2161 cgcgcaccgc gcgcgaagaa agtgtctcga gcctcttcta ttgagcgccg ccgctcggcg
     2221 ggccttcagg tccccacgtc gtgcggtaga ccgccgccgc cgtggcgcta ccgtaggcgg
     2281 caagcgaaat accgacgagg gcgaagaggc accacagcgg cgccccgctc caggccagta
     2341 cgccggtgcc gatggcgatg atgccaagcc ggaggctgga ggccaacaca ggccctagca
     2401 ccctacccgc cccctgcgag gcgaaataaa ggcacaagcc gaggccgaac agcggaaagg
     2461 tcggtccggc gaacctgaga tagaggttgg cggctgccgc aacctcggcg tcggcgctga
     2521 acagtcccga ccagagctgc ggcagcgtcg ccaccagcac gccgatcagc ccgagggcaa
     2581 cggcggaaac ggcagcgccg gtccaggcca cacgccgagc ccggccgatt ctgccggcgc
     2641 ctaccgccat gccgaccatc ggtacgcacg caaccccgac ggcgaaggcg atcggaacca
     2701 gcaagaactc gaggcgcgcg ccgatgccat agccggccaa ggcctccgtg ccgaagctcg
     2761 caattgcccg tgtcaggatc agaaccgtca aaacggactg caggggcgaa aaagccgcga
     2821 gggcaccaac cctcaggatg tcgagaaaca tctcgcggcg ccaagcaatg ccggcaaagc
     2881 gcaacagcaa gcgtccgcga ccggaccgca gataccaaat gagaaccagc gttcccgtgg
     2941 cgcagctgat caccagcccc gccgccacgc cgggcagccc gaagcgcggg atggggccga
     3001 ggccgaggcc gagggcaccg ccaaggccaa tctgcagggc cgatacgccg agcagggtca
     3061 gcgacggcac ctgcatattg ccggtgccgc gcacgatgga ggcgagtgta ttgagcaacc
     3121 acagcaagac cgccgccgac aaggcgacgt tggcgtaggc catggcctgc tccaacaccg
     3181 caccgcgtcc gccgagcgcg gcgaccagct cgcgaccgaa gaggacgaac agcaacgaga
     3241 agaagaggcc cgtgcccagg ccgatcgcca aggcgtgcag cgccagcgcc ctggcgcgcg
     3301 gctcatcacc agcaccgagc gcgcggctga cggcggagga aacgccgccg cccatagcgc
     3361 ccgcggatag catctgcatc agcatgacca tggggaaaac cagggcgatg gccgccaacg
     3421 gcgccgtccc aagcattccg acgtaagagg tttcggcgat ggccacggcg gcggtgacgc
     3481 taagcgcgat caggtttggc agggcgaggc gcaacagcgt cggcagcaag ggcgcctgca
     3541 acagcgcgtt gccgctggca tggcgcgtcc gcagggatgc cgaccccatc gttggtgctg
     3601 cgtcggtcaa gagaagcgca ctccgctggc caggtccagg cgatcgcggc tctgctatag
     3661 aaaataaagt gacttgtcaa tcgatagtca cttgcggttt aggaatgaga ggcgtacatt
     3721 ccctgcatgg ctgcacgata tggccaagtc tgtccggtgg cgcgcgcgct cgatgtgctg
     3781 ggcgaacgct ggtcgctctt gatcctgcgc gatctcaagc gcaagggacc cctgcgtttt
     3841 caggagctgg aggcgggcct gcagggcgcc gcccccaaca cgctgtcggc gcgtctcaag
     3901 gccttggagg cgcatggcgt gatcgcgacc cgtctctatg cgcgtcatcc cccccgttat
     3961 gaatattttc tgacgccaaa gggccaggac ctcgctcccg tgctgaaggc cctttatgcc
     4021 tggggcgagc gtcacgcctg agcccggccc gcgtgcgtgt gtgtgcgcca ggcgccggag
     4081 ccgcggccga agcccgggcg cgcgacacca aaagagagcc agcaaatcgc cgccaggaat
     4141 ttgcggggtt cgccgtgacg aggttcgggt gttttctgcg ctgattgcag gagttacgcg
     4201 cattgccgcg cgctgcggag cttttcagga tggggtcagg ctaacgccac accattcaag
     4261 cgctctcatc tcctggacac ctcttgatcc ccatgcgaat tgcgggttcg gtcggttgcg
     4321 cggcaatttt gttgctgggt ttcagcggtg cggcgatttc cgtgcggatc gacggctcca
     4381 aggacgcccc agccgcgcag cctcgagacg agggcgccgc gccgccggcc ggcgccgggt
     4441 cagcgcagcc accgcagcgc atcgccaacg cggactgggc ggaccggctg cgctcggagg
     4501 acttttggca gcacatgcga tcgccccagt cgcgtcagga cgacgcgtcc cgccgcgccc
     4561 ccgggccgac gcgtttggaa ggacgacacc gctacgcgcc ggtcgaacgc cgcgacgagc
     4621 tgcgctcgaa agcggcgact ttcgcgagcg gcagctatcg cacggtctgc gtgcgcctat
     4681 gcgacggcta tttctggccg ctgagtttcg cggcttcgcg ggccagcctc gccgcggacc
     4741 ggcgcaagtg tgaagagagc tgcgaggcgc ccgcccgatt gtacctcgcc aaagacgcgg
     4801 aaaccccgct cgaggagatg cgcgacgaaa acggtcgcta ctatcgcgat ctgaagaccg
     4861 ctttcatcta tcgcacgtcc tatgtcgaga gctgcaaatg tcgggcccat ccctgggagg
     4921 cggcggcgct ggcccggcac gcgggctacg cccagtccag caaaccgccc cagccgcgtc
     4981 gctgaaccta gagtgccggg ctgacgaaca gcgctggccc gtgccgaggc cgccgcaacc
     5041 cctaggccgc aagccccagc gcgctggcga gctcggccag cgttgccttt tccttatcgc
     5101 tgacgggcgt gccgccaaac cccatgaaac cgccttcctt ggaggcctca gcggcgtttt
     5161 cggcaatgtg atgcagccag gtcttgaacc ccgcagcatc ccccggcgcc ttgctgtcga
     5221 tcagctgcgc cacatcgcgc agcgcgccga tggacttgtt cttcatctcg ccgacggcga
     5281 tccctttgag ccgggattgc acctgcttgc gggctgcctg gcgcccatcg ccgctctcga
     5341 aatcactgac cacggccttg atcagttcat tggaactcgg gtctgctttg gcttgcagca
     5401 acgttcgccc ggttgccatg gagtccttga gaatgccgaa aagcccgctc gggtcggcgg
     5461 cgctcacggc cattcccg
//
LOCUS       DASTMT010000199        13950 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_5305585, whole genome shotgun sequence.
ACCESSION   DASTMT010000199 DASTMT010000000
VERSION     DASTMT010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 13950)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13950)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13950
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5305585"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..399
                     /locus_tag="VFR00_04580"
     CDS             <1..399
                     /locus_tag="VFR00_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HEU0158562.1"
                     /translation="FLPYYAILRAIPNKLLGVIAMFSSIILLCFVPWLDTSRVRSTKY
                     RPLYKGFFWLFIVTVIALGYLGSKPPEGLYVFWARVFTAYYFIHLLLVLPIVGVIETP
                     RPLPRSISEAVLGAAGGPVSVGAASAPEKR"
     gene            449..1288
                     /locus_tag="VFR00_04585"
     CDS             449..1288
                     /locus_tag="VFR00_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019963927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c1"
                     /protein_id="HEU0158563.1"
                     /translation="MGSVLAALWLALAAPVSAAEDAHPEIHRQHWSFGGVFGHFEDAQ
                     LQRGFKVYTEVCARCHGVRRLAFRNLAQPGGPSFPEAAVKSLAATYQVDAEPNEEGKI
                     LKRPATLADHIPPPYQNDQEARFALNGALPPDLSLIARARAAEENTPFYRVPDRMLRD
                     VVTAYQEGGADYVYAYLTGYKEPPQGTKLADFMNYNVAFPGHQTAMPNPFQGGDGLVK
                     YDDATPATVDNYARDVTAFLAWAADPKLEERKRMGLLVVAYIVLTALLLGLAKRRIWR
                     DSH"
     gene            complement(1330..1656)
                     /locus_tag="VFR00_04590"
     CDS             complement(1330..1656)
                     /locus_tag="VFR00_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011087569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIPSNAP family protein"
                     /protein_id="HEU0158564.1"
                     /translation="MIYELRVYSCLPGRLPALLKRFETETLKIWEKHGIKQAGFWTVL
                     IGDGNHDLYYLLAWNSMAEREQKWTTFQADPAWIAARSASEREGPILANVKSMFLAPT
                     AFSAVK"
     gene            1843..2718
                     /locus_tag="VFR00_04595"
     CDS             1843..2718
                     /locus_tag="VFR00_04595"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019963928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HEU0158565.1"
                     /translation="MTKTVLGIIGGSGLYDMPALQSLHWESVPSPWGETSDQILFAEL
                     DGLPLRFLPRHGRGHKLPPSAINYRANIDALKRAGVTDLVSLSACGSLKEDFPPGHFV
                     LVDQFIDRTFARQKSFFGPGCVAHVSMADPVSPGLVDRLEVAAKDLSIPYTRGGTYLV
                     MEGPQFSTRAESHLYRSWGCAVIGMTNMPEAKLAREAEICYATVAMVTDYDCWHAEHS
                     EVDVATVIKVLGENVDKAHALIARLARDFPRQHPPCPIGSDRALEVAIITPPAARDAA
                     LIAKLDAVAGRIVRG"
     gene            complement(2758..3012)
                     /locus_tag="VFR00_04600"
     CDS             complement(2758..3012)
                     /locus_tag="VFR00_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010969964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1272 domain-containing protein"
                     /protein_id="HEU0158566.1"
                     /translation="MLELRPNCECCDKDLPAEAADALICSFECTFCTECAQKRLPGGR
                     CPNCGGELVRRPVRPAEKLAKFPASAKRVLKPHAGCGPPR"
     gene            3081..3545
                     /locus_tag="VFR00_04605"
     CDS             3081..3545
                     /locus_tag="VFR00_04605"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEU0158567.1"
                     /translation="MTFADLIERFARAAAAGDGDALADLFTPDGTYDDYFFGPCRGRA
                     AIKDMLAHFAEGGQNFRWEFYDPALAGETGYASYRFSFESKRKEANGSRVVFDGIGRF
                     DLEGDRIKRYSEVFDRGMALAQQAFEPERLNKIELRYAGALKSRPEWAAHLK"
     gene            complement(3633..5555)
                     /gene="mdoH"
                     /locus_tag="VFR00_04610"
     CDS             complement(3633..5555)
                     /gene="mdoH"
                     /locus_tag="VFR00_04610"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF003962.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HEU0158568.1"
                     /translation="MTSAAAKGLPARPKSVVPEESPRAMPEQVLWVERRNDAQARKRV
                     YAARAALVLLWSSGSLAFAWTLYQVLSVETPTVLQLVFWCLSTACFAWVAVGSASACI
                     GFVALLLQRTSDTLELPAAEAATGARTALLFPVYREDAAQVAATLEAMCRDIAAADAS
                     DRFDVFVLSDTQDLSEREAEHRLYAALRARVGVPVYVRWRTPNAGKKAGNIRDWIERF
                     GAAYPFFIILDADSLMQAESLLRLVAAMAANRRVGLIQSVPRLVGGQSIFARLQQFAA
                     GYYGPIVSAGLAAWHGPGGNYWGHNAIIRTDAFASAAGLPALAGAPPLGGPILSHDFV
                     EAALLRRAGWEVHMVPSLEGSYEGCPPTLKDLMVRDRRWAQGNLQHVRLLGVRGLPLI
                     SRAHLAMGAFSYLASPIWALTLLVGIVLAVQAKYATPTYFGSEVSLFPKWPVFDAQKA
                     LALFLATVLIVHLPKLLGAIWALRNARERQQHGGLARVVAGVLIESVLSTLIAPLLML
                     SQTSAVASILVGRDAGWGAQRRVGEENSLAQLLHQYRWPTMWGLTGAAICWSISPAVF
                     AWMSPIIIGLLLAAPIGRFTARRAGRVLATLLGTLEERCPPQLLIARAALSEAWRERT
                     GARPAGARAPVGMRDG"
     gene            complement(5552..7090)
                     /locus_tag="VFR00_04615"
     CDS             complement(5552..7090)
                     /locus_tag="VFR00_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0009250 - glucan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein G"
                     /protein_id="HEU0158569.1"
                     /translation="MRLLAKAQHLGALLVCLLVGSAATAAAQGPAEPFSHELVQRTAS
                     ELAKKAYEAPAADSAKGAQDLNLNYDQFRQIRFRRERTIWRGESLNFELQVLPTGWLF
                     KAPVEINLVDQGSVRLLSPDNSYFDLGPLAGKLAPEARLGFSGFRLNGPLNRLDLFDE
                     IIVFQGASYFRALSRGQIYGLSARGLALNVGKPEGEEFPQFRRFWIEKPSAGAAKIVI
                     HALLDSPSVTGAYRFEVSPGWPTAIDVQSTLYPRRDLADVGIAPMTSMFLFSGINRAR
                     INDFRPAVHDSDGLAILNGWGEHLWRPLNNPRRLQTSDFLDRNPKGFGLIQRLRTAAG
                     FQDLEADYERRPSAWVEPREDWGGGAVELFEIPSEEEIHDNIVAYWKPAQVLAAGKAH
                     AFNYKLSWPDDVPRSWPGSYVHATRAGLIAGPQRKAGVFQFVVDFKGFVSPSASELPF
                     ARVEASQGAVSAPVVQTNPDIDGLRVSFSFDPKGVATSELRLVLQANDNPVSETWLYR
                     WTKD"
     gene            complement(7209..8912)
                     /locus_tag="VFR00_04620"
     CDS             complement(7209..8912)
                     /locus_tag="VFR00_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017482908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU0158570.1"
                     /translation="MSALPPPMAERVLLLAPRGRDALVAKGILREAHIEAEICLGLPE
                     LLAELTLGADAVVLTEEAVRSSDARELHEWVAAQPAWSDFPFVVLTRHGGGLERNPAA
                     ALLTRTLGNLSFLERPFHPTTLVSVVETALRGRRRQQECRRLNEELELRVQERTSELA
                     AANRQLLTQIEERERVESTLRQLQRLEAVGQLTSGVAHDFNNLLTVVLGNISFLEKGL
                     AKAGIDGNLSQRLTYMRAAAERGARLTDQLLSFSRRQRLEPKVIDLNETVAGMRDLLQ
                     STMGGSIRIQTQLAVDLWPALVDLTQLELAVLNLSINARDAMEMGGTLSVGTANVELA
                     MPFYPEEPPAGDYVAVRVSDTGAGMSKDVRSKAFEPFFTTKEIGKGSGLGLSQVLGFA
                     KQSGGGVRIQSEAGQGTTIDIFLPRADVRPVKIDLAPPRPTLTAKASARLILVDDDNA
                     VREVTAATLRELGYTVLEAGSGGAALELLERSANIDLMLIDFAMPGMSGAEVARRVET
                     TRPALPILFVTGYADRAALEGVSEAQIIGKPFVDTELHEKVRTALARRAPGKIVRLRP
                     S"
     gene            complement(8909..10402)
                     /locus_tag="VFR00_04625"
     CDS             complement(8909..10402)
                     /locus_tag="VFR00_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008969032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HEU0158571.1"
                     /translation="MSNKAEFLPRAHNDIAGLDAVLGGGWARNRLHLLEGSPGTGKTT
                     IALQFLMAGAEQGEAGIYVSLAETEQELRDGAKSYGWSISPLIEIFELVPPESVLDPD
                     QQQSLLYSSDLELGETIKRILESIERVRPKRVVIDSLSEIRLLAQGSLRYRRQILALK
                     HYFARHRSTVIMLDDLTTESTDRAVHSIAHSVVHLDQLAPIYGGERRRLRVIKCRGQS
                     FRSGYHDFIISPGGVQLFPRPVAAEHRQAFAEEVVRSGIDPLDGLLGGGLAAGSSTLI
                     IGPAGTGKSLLVLQYIAAAVCRGEKAALFVFDEELGLLTSRAKALDIDLVAMQSQDRL
                     VLEQMDAAELSPGEFSYRVRTSVDRQNCGIVAIDSLNGYQAAMPEEQFLTLHLHELLQ
                     YLNRRGAATFLTIAQHGMMGEMRQSVDVTYLADSVILLRYFEAFGRVRRAISVVKKRT
                     GAHEDTIRELHISDRGLGLGEPLHEFQGVLRGVPTYVGQSTPLLERS"
     gene            10708..>13950
                     /locus_tag="VFR00_04630"
     CDS             10708..>13950
                     /locus_tag="VFR00_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011502087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEU0158572.1"
                     /translation="MTEELGGPRVQAASEADRLFAGNSDMAARMRGFDWSATSIGPSH
                     AWPQSLRTAVRIMLTSRQPIWIGWGRDLIYFYNDPYKSIIGGKDAWALGRPASQVWRE
                     LWDAIGPMLAEAMTGDEGTYVEEQLLIMERHGYREETYYTFSYSPIPDDAGKAAGIIC
                     ANTDDTQRVIGERQLSLLRELATKTAEARTWQQVCAEAVKALATNPRDLPFALIYLAE
                     PGSGAIALVGAAGIAEDHPAAGGAPTPWPLAQILADQQPRLIADLPAKLDAELPSGAW
                     EQPPAQAVALPIVASGETGRAGVLIVGLNPFRVFDENYRGFLGLVAGQIAAALANAQA
                     YEEERRRAEVLAQLDHAKTAFFSNVSHEFRTPLTLMLGPLEEALAKADGTATSDRNLI
                     ALAHRNGLRLLKLVNALLDFSRLEAGRGEARFEPVDLARFVREVASNFQSATDRAGLG
                     LLLNCAPLPEPVYVDRDMWEKVVLNLLSNAFKFTFAGHIEVALRLGADGRSAEMSISD
                     TGSGIPAEELPHLFERFRRVEGARGRSIEGSGIGLALVQELLKLHGGGITVRSRLGEG
                     TTFTVTVPLGAGHLPAEKIIAGSGSGPTHARAQAYVEEALGWLTDIEHEQPTASGTED
                     LNPREAPAQSRGQLVLLADDNADMRGYVARLLRQSGYRVEAVIDGEPALAAARRLKPD
                     LIVSDVMMPRLDGFQLLAKLREEPALADIPVILLSARAGEEAKIEGLRAGADDYLVKP
                     FSARELLARVETNLKLAGTRRETARVLREEAEIFELLNEVGTVLSAEIELEPAVQIVT
                     DAATKLSGAAFGSFFYNVIDDKGESYTLYTLSGVPREAFASFAMPRNTQVFAPTFRGE
                     GVVRSADITQDPRYGHNAPHKGMPPGHLPVRSYLAVPVIARSGEVLGGLFFGHSQPDV
                     FGERAERIVAAIASQAAIAIDKARLYRAAQEEIERRRKIEAALRESEQSLEHKVGERT
                     LELAASNARLRAEAEERQRAEGRFQVLVDGVTDYAVYMLGPTGIVSNWNTGAQRIKGY
                     TAEEIVGQHYGRFYTEEDRNAGAPERALAIAASSGKFETEGWRVRKDGSRF"
BASE COUNT         2444 a         4594 c         4518 g         2394 t
ORIGIN      
        1 ttcttgccct attacgcaat cctgcgcgcc atcccgaaca agttgttggg ggtgatcgcg
       61 atgttctcct ccatcatttt gttgtgcttc gtgccctggc tcgacacctc gcgcgtgcgc
      121 tccacaaagt accgtcccct ctacaagggg tttttctggc ttttcatcgt gacggtgatt
      181 gcgctcggct acctgggctc caagcctccc gaagggctct acgtgttctg ggcccgcgtc
      241 ttcaccgctt actattttat ccatctgttg ctggtgctgc cgattgttgg cgtcatcgag
      301 acgccaaggc cgctgccgcg ctccatcagc gaggctgtgc tgggggcggc cggcgggccg
      361 gtcagtgtcg gggcggcgag cgccccagag aagcgctagg ctggggagcg acatgacggc
      421 aaggtttgcc tggagggggg cttcgagcgt gggcagcgtc ctcgctgcgc tctggttggc
      481 gctggccgcg ccggtcagcg ccgccgaaga cgcacacccc gaaattcacc ggcagcattg
      541 gagcttcggc ggtgtcttcg ggcattttga ggacgcgcag ctgcagcgcg gcttcaaggt
      601 ctataccgag gtctgcgcgc gctgccacgg cgtgcgtcgg ctggccttcc gcaacctggc
      661 gcagcccggc ggcccaagtt tccccgaggc cgcggtcaag tcgctggcgg caacctatca
      721 agtcgatgcc gagcccaatg aagagggcaa aatcctcaag cggccagcga cgctcgccga
      781 ccacattccg ccgccctacc aaaacgacca ggaggcccgc ttcgccctca acggcgcgct
      841 gccgcccgat ctttccctca tcgcgcgcgc ccgcgccgcc gaggagaaca cgccttttta
      901 tcgcgtgccc gatcgcatgc tgcgcgacgt tgtgacggcc taccaggagg gcggggccga
      961 ctacgtctac gcctacctga cgggctacaa ggagccgcca caaggcacca aactcgccga
     1021 ctttatgaac tacaacgtcg ctttccccgg gcaccagact gccatgccca accccttcca
     1081 gggcggcgat ggcctggtga agtacgacga tgccaccccg gccacggtcg acaactatgc
     1141 gcgcgacgtc acggcctttc tggcctgggc cgccgatccc aagcttgaag agcgcaaacg
     1201 catgggtctt ctggtggtgg cctatatcgt gctgacggcg ttgttgctcg gcctcgccaa
     1261 acgccgcatc tggcgcgatt cgcactgacg gggctgggct cgcgccgcgc cccaaccccg
     1321 cgcagcacct tatttcaccg ccgaaaaggc ggtgggggcc aagaacatgc tcttgacgtt
     1381 ggcgaggatg gggccttcgc gctcgctcgc actgcgcgcc gcgatccagg ccggatcggc
     1441 ctgaaaggtc gtccatttct gttcgcgttc cgccatcgag ttccaggcca atagatagta
     1501 gagatcgtgg ttgccatcac cgatcagcac ggtccagaac cccgcctgct taatgccgtg
     1561 cttctcccaa atcttgaggg tctcagtctc gaagcgtttg aggagcgcgg gtaggcgccc
     1621 cggcaggcag ctgtagacgc gcaattcgta gatcatgtcc atctccactg gcaatgagcg
     1681 cccaatcctg tcccggccgc gccaccgaga caagaccgca tccgctctgc cagtgcgcaa
     1741 cggcggcctg ggcgcgcgcc gcgcgccgcc ctaaactctc gccgaaggcc gacacccgcg
     1801 cttgaggcct ctcaggccag ggaccaacca caggggcaag ccatgaccaa gaccgtgctc
     1861 ggcattatcg ggggaagcgg gctctacgac atgcccgccc tgcagagcct gcattgggaa
     1921 agcgtacctt cgccttgggg cgaaacttcc gatcaaatcc tattcgccga gctcgatggt
     1981 ctgcccctgc gcttcctgcc gcgtcacggc cgcggccaca agctgccgcc gtcggccatc
     2041 aattaccgcg ccaatatcga cgccctgaag cgtgccggtg tcaccgacct tgtctcgctg
     2101 tcggcctgtg ggtcgctgaa agaggatttc cctcccgggc acttcgtgct cgtcgatcag
     2161 ttcatcgatc gcacctttgc gcgccagaag agtttttttg gccccggctg cgtcgcccac
     2221 gtctcgatgg ccgatcccgt cagtcccggg ctggtcgacc gcctcgaagt ggccgccaaa
     2281 gacctctcca tcccctatac gcgcggcggc acctacctgg tcatggaggg tccgcagttc
     2341 tcgacgcgcg ccgaaagcca cctttatcgc agctggggct gcgcggtgat cggcatgacc
     2401 aacatgccgg aggccaagct cgcccgcgag gcggagattt gttacgccac cgtcgccatg
     2461 gttacggact acgattgttg gcatgccgag cacagcgaag tcgacgttgc gaccgtcatc
     2521 aaggtgcttg gcgaaaacgt cgacaaggcg cacgcgctga tcgcgcgctt ggcgcgcgat
     2581 tttccgcgcc aacatccccc ctgtccaatc ggctccgacc gggccctcga ggtcgccatc
     2641 atcaccccgc ccgcggcgcg cgatgccgcc ctgatcgcca agcttgatgc cgtggccggg
     2701 cgcattgtgc gcgggtgaac gggatgggca tgcccggggc gttcggcctg cgcggcctta
     2761 ccttggcggg ccgcagcccg catgcggctt cagcacgcgc ttggccgagg ctggaaattt
     2821 cgccagcttt tcggcgggcc gtaccggccg acgcaccagc tcaccgccac agttgggaca
     2881 gcgcccgccg ggcaggcgct tttgcgcgca ttcggtgcaa aacgtgcatt cgaacgagca
     2941 gatgagcgcg tcggccgcct cggcggggag atccttatcg caacattcgc agttgggtct
     3001 cagttcaagc atcggccttc cttttctcgt tgctggcgct agcatgatgc ccccatcacc
     3061 agcaagcaag ggaggggagt atgacgtttg cggacctcat cgaacgcttt gcgcgggcgg
     3121 cggctgccgg cgatggcgac gccttggccg atctcttcac gccggacggc acctacgacg
     3181 actatttctt cgggccatgc cgcggccgcg cggcgatcaa ggatatgctg gcccactttg
     3241 ccgagggcgg tcagaacttc cgctgggagt tttatgatcc ggcgctcgcc ggagagaccg
     3301 ggtatgccag ctatcgcttc tccttcgagt ccaagcgcaa agaggccaac ggcagccgtg
     3361 tggtctttga cggcatcggc cgtttcgacc tcgagggcga tcgcatcaaa cgctactcgg
     3421 aggttttcga ccgcggcatg gccttggccc agcaggcctt cgagcccgag cggctcaata
     3481 agatcgagct cagatacgcc ggcgccctga aaagccgtcc cgagtgggcg gcgcacttga
     3541 aatgaggtca cctgcacgga accgggcggg ccgcaccgtc gccgctgcgg ctgctcacgc
     3601 tgggccccgc cgcgatgggg cgccaatgct ggctagccat cccgcatgcc gacgggcgct
     3661 ctggcgccgg cggggcgtgc gcccgtgcgc tcgcgccagg cctcgctgag cgccgcgcga
     3721 gcgatgagga gctgcggcgg gcagcgctcc tcgagtgtgc ccaggagcgt tgccagcacg
     3781 cgcccggcac gtcgagccgt gaaacggccg atcggggccg ccaacaggag cccgatgatg
     3841 atggggctca tccaggcgaa gaccgccggc gaaatgctcc agcagatcgc ggcaccggtg
     3901 agaccccaca tggtcggcca gcgatattgg tgcaggagtt gcgccaagct gttctcctct
     3961 cccacgcggc gttgcgcccc ccagcccgca tcgcggccga cgagaatgct ggcgacagcg
     4021 ctggtttggc tcaacatcag caacggcgca atgagcgtgg agagtaccga ttcgatcagg
     4081 acgccagcga cgacgcgcgc aagtccgccg tgctgctggc gctcgcgcgc attgcgcagc
     4141 gcccaaatgg cccccaatag tttcggcaga tgcacgatca gcaccgtcgc tagaaacaga
     4201 gccaaggctt tttgtgcgtc aaacaccggc cactttggaa acagcgacac ctcgctgccg
     4261 aaataggtcg gcgtggcata cttggcctgc accgcgagca caatgccgac cagcaacgtg
     4321 agcgcccaaa tcggcgaggc gagataggaa aaggcgccca tggcgaggtg cgcgcgagag
     4381 atgagcggca gaccgcgcac gccaagcaga cgcacgtgct ggaggttgcc ctgtgcccaa
     4441 cgacggtcac gcaccatcaa gtccttgagt gtcggcgggc acccctcata ggagccctcg
     4501 agggatggaa ccatgtggac ctcccagccg gcccgacgca gcagggcggc ttccacgaag
     4561 tcgtggctga ggatgggccc gcccaggggt ggcgctccgg ctaaggccgg cagtcccgcc
     4621 gcgctggcga aggcgtcggt acggatgatc gcgttatggc cccaataatt tcccccaggt
     4681 ccgtgccagg cggcaaggcc cgccgaaacg atcggcccat agtagcccgc ggcgaattgc
     4741 tgaagacgtg caaaaatgga ctggccgccc accaagcgcg gcacgctttg aatgaggccg
     4801 acgcgccggt tggccgccat ggcggcgacc aggcgcaaca agctctcagc ctgcatgagg
     4861 ctgtcggcat cgagaattat aaagaaggga taggcggcgc cgaacctctc gatccaatcg
     4921 cgaatgttgc cggccttttt gccggcgtta ggcgtgcgcc agcgcacata gacggggacg
     4981 ccgacccgag ctctgagcgc cgcgtagagg cgatgttcgg cctcgcgctc gctcaggtct
     5041 tgcgtgtcgc tcaagacgaa gacgtcgaag cgatcgctgg catcggcggc cgcaatgtcc
     5101 cggcacatcg cctcgagcgt ggccgccacc tgggcagcat cctcgcggta aaccgggaac
     5161 aacagggcgg tgcgcgcgcc cgtcgccgct tcggccgctg gcagctcgag ggtatcgctc
     5221 gtgcgctgca gcaacagggc aacaaagccg atgcaggcac tggccgagcc gacggcgacc
     5281 caggcgaagc aggccgtact caggcaccag aacacgagct gcaggacggt cggcgtctcc
     5341 acggacagca cctggtacag cgtccaggca aacgccaggg agcctgagct ccaaaggagc
     5401 acgagcgccg cgcgcgccgc gtacacgcgt ttgcgcgcct gggcatcgtt gcggcgttcc
     5461 acccagagca cctgctcggg catggcgcgc ggactttcct cgggaacaac cgacttgggc
     5521 cgcgccggca agcccttcgc cgctgcgctc gtcaatcctt ggtccatcgg tagagccagg
     5581 tttcggacac cgggttgtcg ttggcctgca acaccagccg caactcactg gtggcgaccc
     5641 ccttgggatc gaagctgaac gatacgcgca gaccgtcgat gtccgggttg gtctgtacca
     5701 ccggcgcgct taccgcgcct tggctcgcct ccacccgcgc gaagggcagc tcgctcgccg
     5761 agggcgaaac aaaacccttg aaatcgacga cgaactggaa cacgcccgcc ttgcgttgcg
     5821 gtccagcgat caagccggcg cgcgtggcat gcacgtagga gcccggccaa ctgcgcggca
     5881 cgtcatcggg ccaggagagt ttgtagttga aggcatgggc cttgccggcc gccagcacct
     5941 gggcgggctt ccagtaggcc acgatattgt cgtggatctc ctcctccgag ggaatttcga
     6001 acaattcgac ggcacctccg ccccaatcct cacgcggttc gacccaggcg ctcgggcgac
     6061 gttcgtagtc cgcttccaga tcctgaaaac cagccgcagt gcgcaggcgc tggatcaggc
     6121 caaacccctt cgggttacgg tccaggaaat cgctcgtctg caggcggcgc ggattgttga
     6181 gcggccgcca gaggtgctca ccccaaccgt tgaggatggc aagtccatcc gaatcatgca
     6241 ccgccgggcg aaaatcgttg atgcgcgcgc ggttgatgcc cgagaacaga aacatgctgg
     6301 tcatgggggc gatgcccacg tccgccaggt cgcgtcgtgg atagagcgtc gattgcacat
     6361 cgatcgccgt cggccagccc gggctgacct cgaagcggta ggcgcccgtc accgacgggc
     6421 tgtccagcag ggcatggatg acgatcttgg cggcgcccgc ggacggcttc tcgatccaga
     6481 agcggcgaaa ctgcgggaac tcctcgccct cgggcttgcc gacgttgagt gccaggccac
     6541 gtgcggacag gccgtaaatt tgcccgcgcg acagggcacg aaaatagctg gccccctgaa
     6601 agacgatgat ttcgtcgaat aggtccagcc ggttcagggg accgttgagg cgaaagccgg
     6661 aaaaccccag ccgggcctcg ggcgccagct tgccggcgag cgggccgaga tcgaagtagg
     6721 aattgtcggg tgagagcagg cgcacgctgc cctgatcgac gaggttgatc tcgacgggtg
     6781 ccttgaacag ccagccggtc ggcagaacct gcagctcgaa attcaggctt tccccacgcc
     6841 agatcgtgcg ctcgcggcga aacctgatct gacgaaattg atcgtaattg agattgagat
     6901 cctgcgcgcc tttggcgcta tccgcggccg gtgcctcata ggccttcttg gccagctcgc
     6961 tcgccgtgcg ttgcaccagc tcgtgcgaaa agggctcggc tggaccttgc gcggccgccg
     7021 tcgccgcgct gcctaccagc aggcagacga gcagcgcacc cagatgctga gccttggcca
     7081 acaatctcat cggaccatct gttgcacaca acccgcacct gcaaacggcg cctcattgac
     7141 gaggggcgct gatcttgagc gccgaaggct gctggtaacg tggagaaaac tgctttgttc
     7201 cgggtgcctc aagagggcct gaggcgcacg atcttgcccg gtgcccggcg cgccaaggcc
     7261 gtgcgtacct tttcgtgcaa ctcggtatcg acgaacggtt tgccgatgat ttgggcttcg
     7321 cttacgccct ccagggcggc ccggtccgcg tagccggtga cgaataggat cggcaacgct
     7381 ggccgcgtgg tctcgacgcg gcgcgccact tcggcgccac tcatgccggg catggcgaaa
     7441 tcgatcagca tcaagtcgat gttggcgctg cgctcgagga gctcgagcgc ggcaccgccg
     7501 ctccccgcct ctagcaccgt gtagcccagc tcgcgcagcg ttgcggccgt gacttcgcgc
     7561 acggcattgt cgtcgtcgac gaggatcagg cgggcgctgg ccttggcggt caaggttggg
     7621 cgcggcgggg ccaggtcgat cttcaccggt cgcacgtcgg cgcggggcaa gaagatgtcg
     7681 atggtcgtac cttggcccgc ctccgactga atgcggacgc cgccaccgga ctgtttggca
     7741 aagccgagca cctggctgag gccaagaccc gagcccttgc cgatctcctt ggtggtgaag
     7801 aagggttcga aggctttgct gcgcacgtct ttggacatac cggcgcccgt atctgaaacg
     7861 cggacggcca cgtagtctcc ggccggcggc tcctcgggat aaaacggcat cgccagctcg
     7921 acgttggccg tgccgacgct gagcgtgccg cccatctcca tcgcatcgcg cgcattgatg
     7981 gaaaggttga ggacggccag ctcaagctgg gtcaaatcga cgagcgccgg ccacaagtcc
     8041 acggcgagct gcgtctggat acggatgctg ccccccatcg tgctttgcag gagatcgcgc
     8101 atgccggcga cggtctcgtt gagatcgatc accttcggct ccaagcgctg gcgccgcgaa
     8161 aacgacaaga gctgatccgt cagccgggcg ccgcgctcgg ccgcggcgcg catgtaggtg
     8221 agccgttgag acaagttgcc gtcgatgccc gccttggcca gccccttctc caggaaggag
     8281 atgttgccga gcaccacggt gagaagattg ttgaagtcgt gcgccacccc cgaggtcaac
     8341 tgtccgacgg cttccaggcg ctgcagttgc cgcaaggtcg attcgacgcg ctcgcgctcc
     8401 tcgatttgcg tcaagagctg ccggttggct gcggccagct ccgacgttct ttcctgcacg
     8461 cgcagttcca gctcttcgtt gagccgccgg cactcctgtt ggcgtcgccg cccacgcagc
     8521 gccgtctcca ccacgctcac cagcgtggtg gggtgaaacg gccgctcgag aaagctgaga
     8581 ttgcccaagg tgcgggtaag cagcgccgcc gccgggttgc gctcgagccc gccgccgtgc
     8641 cgggttagca cgacgaaggg gaaatccgac caggcgggct gggccgcgac ccattcgtgc
     8701 aactcgcgtg catcgctgct gcgcacggcc tcttcggtca agacgacggc atcggcgccc
     8761 agcgtgagtt cggccaggag ctcggggagg ccgaggcaga tttccgcctc gatgtgcgcc
     8821 tcgcgcaaga tgcccttggc aaccaaagca tcgcgcccgc gcggagcgag cagcaagacg
     8881 cgctcggcca tgggtggagg aagcgcgctc acgacctctc caacaggggc gttgattggc
     8941 cgacgtaggt tggaacgccg cgcagcacgc cctgaaactc gtgcaagggc tcgccgagcc
     9001 ccaggccacg atcgctgatg tgcagctccc gaatggtgtc ctcgtgcgca cccgtgcgct
     9061 ttttcaccac cgagatggcg cgccgtacgc ggccgaaggc ctcgaaatag cgtaacagga
     9121 tgaccgaatc ggcgaggtag gtgacgtcga cagactggcg catttctccc atcattccat
     9181 gctgggcgat ggtgaggaag gtcgcggccc cgcggcggtt caaatactgc aacagctcat
     9241 gcaggtgcag cgtgaggaac tgttcctcag gcatcgcggc ctgatagccg ttgaggctgt
     9301 caatggcgac gatcccgcag ttttgccgat cgacgctcgt cctgacccga tacgaaaatt
     9361 cgccgggcga gagctcggca gcatccatct gctccagcac gagccggtcc tgggactgca
     9421 tggcgacaag atcgatgtcg agcgccttgg cgcgggaggt gagcaggccg agctcctcgt
     9481 cgaacacgaa caacgccgcc ttttcgccgc ggcacacggc cgccgcaata tattgtagta
     9541 cgagcaacga cttacccgtc ccggctggac caataatcaa ggtgctggag cctgcggcta
     9601 ggccaccacc caacagtcca tccagggggt cgatgccgct gcgcacaact tcctcggcga
     9661 aagcctggcg atgttcggcc gccaccgggc gcggaaacag ctgcaccccg ccagggctga
     9721 taatgaaatc atgatagccg ctgcgaaaag attgcccgcg gcacttgatg acccgcagcc
     9781 gccggcgttc gcccccgtag atgggcgcca gctggtccag gtgcacgacg ctgtgggcaa
     9841 tgctgtggac ggcacggtcg gtgctctcgg tcgtcagatc gtcgagcatg atgacggtcg
     9901 agcggtgcct ggcgaaatag tgcttgagcg ccaggatctg gcgacggtag cgcagcgagc
     9961 cctgggccaa gagccggatt tccgagagac tgtcgatcac gacccgcttt gggcgcacgc
    10021 gctcaatcga ttccaagatg cgtttgatgg tctcgcccag ctcgaggtcc gacgaataga
    10081 gcaggctttg ctgctgatcg ggatcgagca cgctctcggg cggtaccagc tcgaaaatct
    10141 cgatcagcgg gctgatcgac cagccgtacg atttggcgcc gtcgcgcagc tcctgctcgg
    10201 tttcggccag gctgacatag atccccgcct cgccctgctc ggcgcccgcc atcaggaatt
    10261 gcagggcaat ggtggtttta cccgtgcccg ggctgccttc cagcaggtgc aggcgattac
    10321 gcgcccagcc gccaccgaga acggcatcga ggccggcgat atcgttgtgc gcacgcggca
    10381 agaattcagc cttattgctc atcggggtta cccggacctc gtctcctcag gatgaaaagg
    10441 ccactcgagc gttaccggct gagtggaggc gcaaattcgc agccccataa gtgggagccg
    10501 gccaaaaagt tccagccgag ctcgcgctca gccgcggaac ccgaaagcgc ctcgtgcgtt
    10561 ggtctcgctg gggcggaggt ctccctggcc gaacatcgat gttttcgtcc gcgcagtcag
    10621 cgcggcgcgg cttcgctatg ggcgggtaga gttgcggccg gatgggtgtt gatgggcgag
    10681 cgcgcatgcg gtgcaggtga gttggcgatg acggaagagc tgggtgggcc tcgtgtccag
    10741 gctgcctcgg aggccgaccg cctgtttgcg ggcaacagcg acatggcggc ccgcatgcgc
    10801 gggttcgact ggagcgccac ctcgattggg ccgtcccacg cctggccgca aagtctcagg
    10861 acggcggtgc gcattatgct cacctcgcgc cagccgatct ggatcggttg gggcagggac
    10921 ctgatctatt tctataacga cccctacaag tcgattatcg gcggcaagga tgcctgggcg
    10981 ctgggtcgcc ccgcatcgca agtctggcgc gagctctggg acgctatcgg tcccatgctg
    11041 gcggaggcca tgacgggcga tgagggcacc tacgtggagg agcagctcct gatcatggag
    11101 cgccatggtt atcgcgagga gacctactac accttttcct attccccgat ccccgacgat
    11161 gctggcaaag cggccggcat tatctgcgcc aatacggacg atacccagcg agtgatcggt
    11221 gagcggcaac tgtccttgtt gcgggagctg gcgaccaaga ccgccgaggc ccgcacctgg
    11281 caacaggtgt gtgccgaggc cgtcaaggcg ctcgccacca atccgcgcga cctgcccttc
    11341 gccctcatct accttgccga accgggcagc ggtgccatcg ccctggtggg agcggccggt
    11401 atcgccgaag accaccctgc ggcggggggc gcgcccacgc cgtggccgct cgcgcagatc
    11461 ttggctgatc agcagccccg gctgatcgcg gacctccccg ccaagctcga tgccgagctg
    11521 ccaagcggcg cctgggaaca gccgcccgcg caagccgtcg ccttaccgat cgtggcgtcc
    11581 ggcgagactg ggcgcgccgg cgttctcatc gtcggcctca accccttccg cgtctttgat
    11641 gagaattacc gcggtttcct cgggctggta gcgggccaga tcgccgcggc gctggcgaac
    11701 gcgcaggcct acgaagaaga gcggcgccgc gccgaagtct tggcccagct cgatcacgcc
    11761 aagacggcgt tcttctccaa cgtgagccac gagttccgca cgcccttgac gctgatgctg
    11821 ggcccactgg aggaggcctt ggccaaggcg gacggcacgg cgacatcgga tcgcaaccta
    11881 atcgcgctgg cgcatcgcaa cgggctgcgc ttgctcaaac tcgtcaacgc actgctcgat
    11941 ttttcgcggc tggaggccgg tcgtggcgag gcccgcttcg agcccgttga tcttgcccgc
    12001 ttcgtgcgcg aggtcgcctc gaatttccag tccgccaccg accgggccgg cctcggtttg
    12061 ctgctcaatt gcgcaccgct gcccgaaccg gtctacgtcg atcgcgacat gtgggaaaag
    12121 gtcgtcctca acctcctttc caatgccttc aagttcacct ttgccgggca tatcgaggtc
    12181 gccctgcggc ttggcgccga cggtcgcagc gccgaaatgt cgatcagcga taccggcagc
    12241 ggcatcccgg ctgaggaatt gccgcatctt tttgagcgct ttcgccgggt cgagggcgcg
    12301 cgcgggcgca gcatcgaggg cagcggcatt ggcttggccc tggtgcagga gctcttgaag
    12361 ctgcacggtg gcggcatcac ggtgcgcagc cggctaggcg aaggaacgac ctttaccgtg
    12421 acggtgccgc tgggtgcggg ccacctgccg gcggagaaga tcattgccgg tagcggcagc
    12481 ggccccacac acgcccgtgc ccaagcctac gtcgaggagg cgctgggttg gctcaccgac
    12541 atcgagcatg agcagccgac cgcctcgggg accgaagatc taaacccccg cgaggccccg
    12601 gcacaaagcc gtggccagct ggtcctgctt gccgatgaca atgccgatat gcgcggctac
    12661 gtggcgcgtc tgctgaggca gtcgggctat cgcgtcgagg cggtcatcga cggcgaaccg
    12721 gcacttgccg cggcgcgccg gctcaagccc gaccttatcg tatccgatgt gatgatgccg
    12781 cgcctcgatg gctttcaatt gctggcgaag ctgcgcgaag agcccgcatt ggccgacatt
    12841 cccgtcattc tgttatcggc gcgtgccggc gaggaagcga aaatcgaagg cctgcgcgcg
    12901 ggcgccgacg actatctcgt caaacctttt tcggcgcggg agctgctggc gcgggtggaa
    12961 accaacctga agcttgccgg cacgcgccgg gaaacggcgc gcgtcctgcg cgaggaggcg
    13021 gagatcttcg agctgctcaa tgaggtgggc accgtgcttt ctgccgagat cgagcttgag
    13081 cccgccgtgc agatcgtcac ggacgcggcc accaagcttt ccggtgctgc cttcggctcg
    13141 ttcttctaca atgtgatcga tgacaagggg gaatcctata ccctctacac gctgtcggga
    13201 gtaccgcgcg aagcgttcgc ctcctttgcc atgccccgca atacgcaagt atttgcgccc
    13261 acctttcgcg gcgagggagt tgtccgctcg gccgatatta cgcaagaccc gcgctacggc
    13321 cacaacgctc ctcacaaggg catgcccccc ggacacttgc cggtgcgcag ttatctggcg
    13381 gtccccgtca ttgcgcgctc cggcgaggtt ctgggcgggt tattttttgg gcactcgcag
    13441 ccagatgtgt tcggcgagcg cgccgagcgc atcgtcgcgg ccatcgccag ccaggctgcc
    13501 atcgccatcg acaaggcgcg gctctatcgg gcggcgcaag aagaaatcga gcgtcggcgc
    13561 aagatcgaag ccgctttacg cgaaagcgag cagagcctcg agcacaaagt cggcgagcgc
    13621 acgctggaac tggcggcctc caatgcccgg ctgcgtgccg aagccgagga gcgacaacgg
    13681 gcggagggcc gcttccaggt ccttgtcgat ggcgtcaccg attacgccgt ttatatgctc
    13741 ggccccaccg gcatcgtcag caattggaat acgggggcac aacgcatcaa gggctacacc
    13801 gccgaagaga tcgtcggtca gcactacggc cgcttctata ccgaggagga ccgcaacgcc
    13861 ggcgcccccg agcgtgcgct ggcgatcgcg gcgagcagcg gtaagttcga gaccgaaggc
    13921 tggcgcgtgc gcaaggacgg ctcgcgtttc
//
LOCUS       DASTMT010000200         3456 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_762814, whole genome shotgun sequence.
ACCESSION   DASTMT010000200 DASTMT010000000
VERSION     DASTMT010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3456)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3456)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3456
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_762814"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..193)
                     /locus_tag="VFR00_04635"
     CDS             complement(<1..193)
                     /locus_tag="VFR00_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008124279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HEU0158573.1"
                     /translation="MAEANEIKASARPRAGKGGARAVRRQGHVPGVLYGDKAEPQNIA
                     LDFKDLVLAVGRGKFLSTVH"
     gene            complement(431..1207)
                     /locus_tag="VFR00_04640"
     CDS             complement(431..1207)
                     /locus_tag="VFR00_04640"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU0158574.1"
                     /translation="MPAPPPIGLFRGEAALVTGSASNIGRAIALALAREGAAVRCVDL
                     DADRNDAVVAEIRQAGGQAEAVTADLSSAQGWRSALPPEGSPCAMFVHSASPARKESD
                     GALAVTEETFDQMLNTNVRSGFLLARELGQRMRAAAIKGRMLFITSLHAETPRNIPHY
                     SAAKAAQTMLVKELARALGPAGIRVNALAPGAVPGGGFNAAAFDFAGKVPLGRLGRAD
                     DMADMALALLSDRFAGYVTGTTVVVDGGIALYNWIPLPTA"
     gene            complement(1217..1807)
                     /locus_tag="VFR00_04645"
     CDS             complement(1217..1807)
                     /locus_tag="VFR00_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013949497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor
                     SspB"
                     /protein_id="HEU0158575.1"
                     /translation="MTAEPHIDYEALAQVAMRSMVRMVLARVAKTGLPGEHHFYIAFD
                     TQAPGVSISKRLREKYPKEMTVVLQHRFWDLGVSDDRFEVKLTFDGIPERLSVPFAAI
                     KVFFDPSVPYGLQFEDSDLAGEPLGSAETTTVEAQPGKLEKKPRVPRKPRAEKAAEPP
                     RPERPVGRPQIVATKPESGDASNENKVVSIDAFRKK"
     gene            complement(1850..2210)
                     /gene="ssrA"
                     /locus_tag="VFR00_04650"
     tmRNA           complement(1850..2210)
                     /gene="ssrA"
                     /locus_tag="VFR00_04650"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01849"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="alphaproteobacterial-type; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01849"
     gene            2278..3069
                     /locus_tag="VFR00_04655"
     CDS             2278..3069
                     /locus_tag="VFR00_04655"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HEU0158576.1"
                     /translation="MESQPSKPSRGTGGLLLGGPAYRAQTPWGPGWAVLAAALITLVG
                     ILAAVFFVGAANLSAPAEAESPMVRGHIALTTLGVWQGATIALTLVASLLFGGRPGDV
                     LALRAPRAGAIVYLSAILALVVLQLLVSVVQYFLVSGDMFADLRPFVDVAIGPNWGLA
                     LLVVGIGAPLSEELLFRGFLLSALAKTRLGFAGAALISTTLWTALHAGYSLIGILEVF
                     TIGLFFSWMLWRTGSLRVPIFCHALYNSLIVCMLRFVALPGASIG"
     gene            3096..>3456
                     /gene="thyA"
                     /locus_tag="VFR00_04660"
     CDS             3096..>3456
                     /gene="thyA"
                     /locus_tag="VFR00_04660"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEU0158577.1"
                     /translation="MQAYLDLLRRVRSQGVRKSDRTGTGTLSVFGHQMRFDLNAGFPL
                     VSTKKVHLKSVIHELLWFLRGDTNTAYLKAHGVSIWDDWADANGDLGPIYGAQWRSWP
                     AGDGRAIDQIAEVVGELK"
BASE COUNT          561 a         1138 c         1136 g          621 t
ORIGIN      
        1 cgtgcactgt tgagagaaac ttgccacgcc cgacggcgag cacgagatcc ttgaagtcga
       61 gagcaatgtt ttgcggctca gccttgtcgc cgtagagcac accgggaacg tgaccctgac
      121 ggcgcaccgc ccgcgctccg cccttgccag cacgtggacg cgcggacgct ttgatctcat
      181 tggcctcagc catggcgacc aactcctgat ggaagagggg ccggcgcgca tcgcgccgag
      241 gagccctaat tggcgggcgt tatagcggcg ggggccgcgc tgtgcaaccg ggcagcgcgc
      301 cggggccggg cggccacccc tccgcagcct gccgccacct tgccaccgcg ctgcccgggc
      361 gcgcgggcag ccacgatggc gaccgtcacg gtggcacgaa aacaggcaaa aaatcgcgcg
      421 cgccgcgggg ctaggccgtc ggcagcggga tccagttgta gagcgcaata ccgccgtcca
      481 ccaccaccgt tgtccccgtg acatagccgg caaagcggtc cgagagcagc gctagcgcca
      541 tgtcggccat gtcatcggcc cggccgagcc ggccgagtgg aaccttgccg gcgaagtcga
      601 aggcggcggc attgaagccg ccgccgggga ccgcgcccgg cgccagcgca ttgacgcgga
      661 tgccggcagg gcccagggca cgggcgagct ccttgaccag catggtctgt gcggccttgg
      721 cggcggagta atggggaatg ttgcgcgggg tttctgcgtg cagcgaagtg atgaagagca
      781 tgcgaccctt gatggcggca gcgcgcatgc gctgcccgag ctcccgcgcc agcagaaatc
      841 ccgagcgcac gttggtgttg agcatctgat caaaggtctc ttccgtgacc gcgagtgccc
      901 catcgctctc cttgcgcgcc ggcgaggcgg agtgcacgaa catggcgcag gggctgccct
      961 cgggcggcag ggcgctgcgc cagccctggg cgctgctaag atcggccgtc accgcctcgg
     1021 cctggccgcc ggcttggcga atctcggcga ccacggcgtc gttgcgatcg gcatccagat
     1081 cgacgcagcg cacggccgcg ccttcccgtg ccaaggcgag cgcgatagcc cgaccgatat
     1141 tggaggccga tccggttacc agcgccgcct cgccgcgaaa gagcccgatc ggaggtggtg
     1201 ccggcatggg ccaggtttac tttttgcgga aggcgtcgat gctgacgacc ttgttctcgt
     1261 tgctggcatc accggactca ggcttggtcg ccacgatctg cggtctgcca accgggcgct
     1321 cgggacgcgg cggctcggca gccttttccg cgcgcggctt gcgcggcacc cggggcttct
     1381 tctcgagctt gcccggttgc gcctcgaccg tcgtcgtctc ggcggagccg agcggctcgc
     1441 cggcgagatc ggaatcctca aactgcaggc cgtagggaac gctcggatcg aaaaacacct
     1501 tgatggccgc gaagggaacg gacagtcgct cggggatgcc gtcaaacgtg agcttgacct
     1561 cgaagcgatc gtcgctgaca cccaaatccc agaagcggtg ttgcaacacc accgtcattt
     1621 ccttgggata cttttcgcgc aggcgcttgg agatcgacac cccgggggcc tgggtgtcga
     1681 aagcgatgta gaaatggtgc tcgcccggca ggcctgtctt ggccacgcgc gccagcacca
     1741 tgcgcaccat cgagcgcatg gcgacctgcg ctagcgcttc gtaatcgata tgcggctcag
     1801 ccgtcatgca ccaacaaaag gccacgggac cagccgtggc gaaaagagta gtggagggct
     1861 tctgttgccc ggtgccctcc gataccgcgc ctgccgttgc tagacgtcag ggcttggatt
     1921 tagacgtttc gcgccgcgtt acgcagcgag agccgtctca gcatagttgt cattggcaac
     1981 tattctgatt gacccgataa cggcggtatc atgccgagca aaagcgcacc ctttacaccc
     2041 tcgtcgatcc tagttcgccc ccgccacatg ccgccgggag tgcggcatct ggtggaggcg
     2101 ccgggtactg cccccgggtc cgataggctt attacggtga ccatttatcg ccatagccgg
     2161 acgaatccgg cccctctaat ataggaaaac ttggccccaa aaaaaagctt cccaattcga
     2221 ggcctgccag ccaaagggat ggtgggatcg caatgagcgt ttgcggcgag gaactgtttg
     2281 gaaagtcagc catctaagcc ctctcggggc accggcggcc tgcttctggg tgggccagcc
     2341 taccgtgccc agacgccctg gggcccgggc tgggccgtgc tggcggcggc gctgatcacg
     2401 cttgtgggca ttctggcagc ggtgtttttc gtcggtgcgg ccaatctcag cgcacctgcg
     2461 gaggccgaga gcccgatggt gcgcggacat attgccctca ccacgctcgg cgtgtggcag
     2521 ggggccacca tcgccctgac gttggtggcc agcctgctgt tcggcggccg ccccggcgac
     2581 gtcctcgccc ttcgggcccc gagggccggc gccatcgtct acctgtcggc gatcctggcc
     2641 ctggtggtgc tccagctact ggtgagcgtt gttcaatact ttttggtgtc gggcgacatg
     2701 tttgccgatc tgcgcccctt cgtcgacgtt gccatcggcc cgaactgggg cttggcgctg
     2761 ctggtggttg gcatcggcgc gccgctgtcg gaggagttgc tgtttcgcgg tttcctgctc
     2821 tcggcgctgg ccaaaactcg cctcggcttt gccggtgcgg cgctcatcag cacgacgctg
     2881 tggacggcct tgcatgccgg ctactccctg atcggcatcc tcgaagtctt caccatcggc
     2941 ctcttctttt cgtggatgtt gtggcgcacg ggcagcctgc gggtgccgat tttctgccat
     3001 gcgctctata actccttgat cgtgtgcatg ctccgcttcg tcgccctccc cggcgcgtcg
     3061 attggctagc ccaggaatcg cactaaagta acggcatgca ggcctacctc gacctcctgc
     3121 gccgcgtgcg cagccaaggc gttcgcaaaa gcgaccgcac cggcaccgga acgctcagcg
     3181 tcttcggcca ccagatgcgt ttcgacctca acgccgggtt cccgctggtc agcaccaaga
     3241 aggtccatct caagtcggtg atccacgagc tgttgtggtt cctgcggggc gacaccaaca
     3301 cggcctatct caaggcgcac ggggtcagca tttgggacga ctgggccgac gcaaacggcg
     3361 accttggacc gatctacggc gcccagtggc ggtcctggcc cgccggcgat ggccgcgcca
     3421 tcgatcagat cgccgaggtc gtcggcgagc tcaagc
//
LOCUS       DASTMT010000201         1717 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_1093167, whole genome shotgun sequence.
ACCESSION   DASTMT010000201 DASTMT010000000
VERSION     DASTMT010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 1717)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1717)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1717
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1093167"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1417)
                     /locus_tag="VFR00_04665"
     CDS             complement(<1..1417)
                     /locus_tag="VFR00_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006022483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HEU0158578.1"
                     /translation="MARRSASARNEPEFDIDVAPAVSIDPAKDRAARPPATKARDKGD
                     PDSEPAPRRSKAKSESKRDKSGSGKRRRGGGGGFGWRRALYWAVVLALWVVIAGIGTI
                     VWVGMHLPPLQSLEIPRRPPAIQILAMDGKPLAIRGEGNGSALTLKDMPPYLPKAFLA
                     IEDRRFYQHRGIDPVGLVRALAANALHRGVSQGGSTITQQLAKNLFLTQERTMMRKLQ
                     EVVLALWLEHKFSKDQILEIYLNRVYFGSGAYGVEAACQRYYGKSARQATLAEAAMLA
                     GLVKSPSRLAPTRNYDAAQRRSQAVLAAMAAEGFAKEDAVKLALAHPPAIVQPSAGGS
                     ANYVADFVLDVLNDLIGHPEQDVVVETSIDPALQTAAEGALVDVLAAKGAKFGVSQGA
                     VVAMTPDGAVRALVGGKNYNESQFNRAVSAKRQPGSSFKPFVYLTALEHGLTPESVRE
                     DRPIALKGWKPENYRHEYFGPV"
BASE COUNT          277 a          547 c          575 g          318 t
ORIGIN      
        1 tgaccggtcc gaaatattcg tgacgatagt tctcgggttt ccagcctttc agcgcaatcg
       61 gtcggtcttc acgcacgctt tccggcgtga gcccgtgctc cagcgcggtc aaatagacga
      121 acggcttgaa tgaggatccg ggctggcgct tggccgagac cgcacgattg aactggctct
      181 cgttatagtt cttgccgccg accagggcgc gcaccgcgcc gtcgggcgtc attgcaacaa
      241 cagcaccttg gctgacgccg aacttcgcac ctttggccgc aagtacatca acgagcgcac
      301 cctcggcggc ggtctgcagc gcgggatcga tcgaggtctc gaccacgacg tcctgttccg
      361 gatgcccgat cagatcattg agcacatcga gaacgaaatc cgccacgtag tttgcggagc
      421 cgccagcact cggctggacg atggccggcg ggtgcgccag cgcaagcttg accgcgtcct
      481 ccttcgcgaa gccttccgcc gccatggcgg cgagcacggc ctgggagcgg cgttgcgcgg
      541 cgtcgtaatt gcgggtcggc gcgaggcgcg acggcgattt gacgagaccc gcgagcatag
      601 ccgcttctgc caaggtcgcc tggcgggcgg acttgccata gtagcgctgg cacgctgcct
      661 cgactccgta ggcgccggac ccgaaataga cgcggttgag ataaatctcg aggatctggt
      721 ccttgctgaa tttgtgctcg agccacagcg ccagcaccac ctcctgcagc ttgcgcatca
      781 tggtgcgctc ctgcgtgagg aacaggttct tggcgagctg ttgcgtgatg gtcgagccgc
      841 cttgcgagac gccgcgatgt agcgcgttgg cggcgagcgc gcgcacgagg ccgaccggat
      901 cgatgccgcg atgttgatag aagcgccgat cctcgatcgc caggaaggcc ttcggcaagt
      961 atggcggcat atccttgagg gtcagggccg aaccgttgcc ctcgccgcgg atcgccagcg
     1021 gcttgccgtc catggcgagg atctgaatcg cgggcgggcg tcgcgggatc tccagcgatt
     1081 gcagcggcgg caggtgcatg ccgacccaca cgatcgtgcc gataccggcg atcaccaccc
     1141 acagcgcgag gaccactgcc cagtagaggg cgcggcgcca gccaaagccg ccgccgcccc
     1201 cgcgccggcg tttacccgag ccgctcttgt cgcgcttgct ctcagatttg gctttgctgc
     1261 gccggggggc gggctcgctg tctgggtcgc ccttgtcgcg cgccttcgtc gccggtgggc
     1321 gcgcagcgcg atccttggcg ggatcgatgc tgacggccgg cgcgacgtcg atatcaaact
     1381 caggctcgtt gcgcgctgag gcgctccgcc gtgccaaccc cgtgccccat ccgctacccc
     1441 ggtatccggg gccggcatga tgcagcgcgg ccgtttaagg tgcggttaag gtgggcgccg
     1501 gccttgttcc aggcggccgt ccgtgggcgc ccttcaggat gatcgcacta ggcgttcaag
     1561 ttttattgcc cgcacgcacg caggctaagc gtaagacggt ttgccgcaca gaagggcgct
     1621 tgaaggctat tgtgcactgc accatatcgc cggcaatggc gctttgcctg cttgtggaag
     1681 gtgagtgatg gaagtcgagt tgattggcgc catggcg
//
LOCUS       DASTMT010000202         9504 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_1093315, whole genome shotgun sequence.
ACCESSION   DASTMT010000202 DASTMT010000000
VERSION     DASTMT010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 9504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9504
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1093315"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1180
                     /gene="lptD"
                     /locus_tag="VFR00_04670"
     CDS             <1..1180
                     /gene="lptD"
                     /locus_tag="VFR00_04670"
                     /inference="COORDINATES: protein motif:HMM:NF016348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0010033 - response to organic substance
                     [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HEU0158579.1"
                     /translation="VIDYNYIVNQPVLGGELSFTGHARSMSRNNGGTDTTHALLEANW
                     RRKMIDPVGQVWTPFVNARGDVYSYNDAFNPALATPTPIPNDTVLRGIAAAGVLYSYP
                     FVAHTADASHVIAPTAQIIVRQNKVNQFFLPDEDAKSLVFDDTLLFDIDKFSGYDRFE
                     TGTRANVGLQYTFQANSGFNARAVFGQSYQLSGDNPYNNPGFDPLGCQTPSPSGCPNF
                     NPQNGLETNRSEYVAGLYLSPFAGFNVVAQGRFDERDWSLRRQDLLVNANYGPVIASV
                     AYSLSRFDPSTGTQSTQQDVMPYLGLKITNNWSIGGSMRYDIDAKERIQDQLQLKYAD
                     ECFVLTASYTETFIENPSLDLRPDRTLMLRFELKHLGEFNYKTDVLSHVFGDSNQGPQ
                     "
     gene            1344..2543
                     /locus_tag="VFR00_04675"
     CDS             1344..2543
                     /locus_tag="VFR00_04675"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HEU0158580.1"
                     /translation="MHQPVFRRSVPAALLASALLMLGSPPLSAAQTGKAQTAAVEKQG
                     AAAASKAGGQSIVALVNDEPVTAYEIEQRARFLSLSSNIGDKVKENFKRLAEQESTNQ
                     QLRAIMQQVIDSNKGKAPDQLRAIFEEKKKQFSMTLERQAIESARSGLVPQMRKGAQE
                     ELIEERLKLQEAKKNGIEVSDEEVRRVFKGLADNNKMTEEQFVQHLKGLGVDANTLKE
                     RFRAMIGWREVIRRRFSALVSVTQREIDRAVSASGDGADDGVELHVQKMTLSLPTASD
                     QAQMAKRFAEAEALRRKFGGCKSMAGLAKEAPGVKFDDMKFIKPSSISEPTRSLLLSA
                     KDGDMLPPSATPGAIDLLAVCARRTVKGDEKQREKVQEDLRAKEFEMLAKRHLRDIRQ
                     DAHIEFR"
     gene            2521..3591
                     /gene="pdxA"
                     /locus_tag="VFR00_04680"
     CDS             2521..3591
                     /gene="pdxA"
                     /locus_tag="VFR00_04680"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HEU0158581.1"
                     /translation="MPTSNSDEAHAAAPAHAAAAPELPPLAVTMGDPAGIGPDITLIS
                     WLNRAALALPPFVLLGDADLVAERASQLGLAIPIERVGEFAAGREAFARALPVLPVRP
                     AGADQSPIVAGIEQAVLAVLEGRALALVTNPLTKSALNPAGLSYPGHTDYLALLAERF
                     AGGPRPRPVMMLAAEELKVVPATVHVPIAAVPTLLTRELLSETIAITAAALSQDFGLV
                     APRIAVTGLNPHAGEDGLIGKEERELIGPVLASLAASGLGVTGPHAADTLFHAAARRS
                     YDAVVAMYHDQALIPLKTLAFDRGVNITLGLPFVRTSPDHGTAFSLAGTGKARPGSFV
                     AAVRLAAEIGARRRAALGPQRR"
     gene            3588..4466
                     /gene="rsmA"
                     /locus_tag="VFR00_04685"
     CDS             3588..4466
                     /gene="rsmA"
                     /locus_tag="VFR00_04685"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013418562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEU0158582.1"
                     /translation="MTRVAAATPDGLPPLGEVIRSLELAARKSLGQNFILDLNLTRRI
                     ARAGGSLAGRSVVEVGPGPGGLTRALLLEGASRVVAIERDERCGPALAAIAQRYPGRL
                     SVQFEDALKTDWSKLAAALAPERPLIAANLPYNVATLLLIGWLESEPWPPWYRGMVLM
                     FQKEVAQRITAPVGTKAYGRLAVIAQWRCRVRLLFTLKPEAFTPPPTVASALVAFEPV
                     ERPQPACSVASLGRVTAAAFGQRRKMLRQSLKPLLADTEALLGAAGIAPTLRGEALSV
                     SEFARLAYVLERWPGR"
     gene            complement(4475..5128)
                     /gene="gmk"
                     /locus_tag="VFR00_04690"
     CDS             complement(4475..5128)
                     /gene="gmk"
                     /locus_tag="VFR00_04690"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018645374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEU0158583.1"
                     /translation="MSEAGPQAGRIERRGLLLVLSSPSGAGKTTLASGLLKSDATLRM
                     SVSVTTRAPRQGERDGKDYSFIDQPEFERRKQAGELIEWAEVHGNFYATPRAPVLDAL
                     ARGEDMLFDIDWQGARQLRAQLGADMVGVFILPPDGATLERRLQTRAQDSQAVVSRRL
                     AAAASEIAHWRDYDYVLVNADLATSLADLRAILAAERLQRARQTGLAGFVAGLLGQL"
     gene            complement(5121..5993)
                     /locus_tag="VFR00_04695"
     CDS             complement(5121..5993)
                     /locus_tag="VFR00_04695"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HEU0158584.1"
                     /translation="MTGFAARDGTAGPAAWHWELRSVNSRGLDVRLRLPPGLESLEPR
                     IRAAITQHLARGSIAVNLSAKRSDGGAEIRLNEAALRQVLLALERLKGMVEVAPPRAE
                     ALLGIKGVLELIEPEETTEEVSARGEAMLRDLERALVELVAVRTAEGGRLEQIVLDQL
                     AVIERLTQRIAVLPARSIEAVRARLKEQVGRLLESNVNFDEARLYQEAAMLAARADVE
                     EELQRISSHVAAARELLAGDAPAGRRLDFLAQELNREANTLCSKASDEDTTRAGLDLK
                     AVIDQMREQVQNIE"
     gene            complement(6121..7455)
                     /gene="mltG"
                     /locus_tag="VFR00_04700"
     CDS             complement(6121..7455)
                     /gene="mltG"
                     /locus_tag="VFR00_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEU0158585.1"
                     /translation="MRNRHPADRHADPKTSRTRGSLARSPAERLEPTRPARVNRKIRL
                     EEEHRPRPVLAFMNGLLTVALVGMLLLGALAYYFDGQMDAPGPLERNKVIVIPKLEGS
                     LEIAARLEREGVISDRRLFTAGYRWLQLLARLEGDKTLQLKAGDYEVRPHASVRELID
                     LICDGKTVTYKITVPEGLTSQQIVDRLLADANLSGEISATPAEGSLMPETFVIQRGAQ
                     REAVLESMAAESKKLMERMWAQRKKDLPVKTWDEAVTLASIVEKETGRNDERDRVAAV
                     FINRLRQNMRLQSDPTILYGLYGGKVAWGRPIQRNEITQKTPHNTYQIDGLPPTPICN
                     PGRRAIEAVLQPRETRELYFVADGSGGHAFAETLRDHNANVQKWRLVEKDLKAKAAPE
                     PPAEEPTPKLRPGVKQRAAARAKAEAKPEAAADKAPAGAATTADPATDPKRR"
     gene            complement(7807..9072)
                     /gene="fabF"
                     /locus_tag="VFR00_04705"
     CDS             complement(7807..9072)
                     /gene="fabF"
                     /locus_tag="VFR00_04705"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013635837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEU0158586.1"
                     /translation="MPRRVVITGLGLVTPLGCGVEASWKRLIAGESGARRVEEFEVSD
                     LSCQVACFIPRGPLSEGKFNPDDWMEPKEQRKVDDFIIYGMAAATQALEDAGWRADTT
                     EKQERTGVLIGAGIGGLNGIAETSILLKEKGPRRVSPFFIPGRLINLASGYVSIKHAL
                     KGPNHAVVTACSTGAHAIGDAARMIALDDADVMLAGGAESPICRIAIAGFAACRALCT
                     TFNDQPQKASRPYDKDRDGFVMGEGAGVVVLEELEHAKARGAQIYAEVVGYGLSGDAY
                     HITAPSEDGDGAYRSMRMALQRAGVEPSEVHYINAHGTSTMGDEIELAAVERLFGNAA
                     GKLAMSSTKSAIGHLLGAAGAVEAIFSVLAIRDNIAPPTLNLDHPSVDTPVNLVPHKA
                     QARRIDTALSNSFGFGGTNASLVLQRFKA"
     gene            complement(9169..9411)
                     /locus_tag="VFR00_04710"
     CDS             complement(9169..9411)
                     /locus_tag="VFR00_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEU0158587.1"
                     /translation="MSDVAARVKKIVVEHLGVEADKVTDSASFIDDLGADSLDTVELV
                     MAFEEEFGCEIPDDAAEQIQTVGDAVKFLEKHASAA"
BASE COUNT         1519 a         3283 c         3097 g         1605 t
ORIGIN      
        1 cgtcatcgac tacaactata tcgtcaacca gccggtgctc ggtggcgagt tgagcttcac
       61 gggccatgcc cgctcgatgt cgcgcaacaa cggcggcacc gacaccacgc atgccctgct
      121 cgaggcgaac tggcgccgca agatgatcga ccccgtcggg caggtctgga cgcccttcgt
      181 caacgcccgc ggcgacgttt acagctataa cgatgccttc aacccggctt tggcaacgcc
      241 gaccccgatc ccgaacgata cggtgctccg aggcatcgcc gcggccggtg tgctttattc
      301 ctacccgttc gtggcccaca ccgcggatgc ctcccacgtc attgcgccaa ccgcgcaaat
      361 catcgtgcgg caaaacaagg tcaaccagtt cttcctcccc gacgaggacg ccaagagtct
      421 cgtgttcgac gacacgctct tgttcgatat cgacaaattc tcgggctacg accggttcga
      481 gacgggcacg cgggccaacg tcgggctgca atacaccttc caggccaaca gcggcttcaa
      541 cgcgcgcgcg gtctttggtc agagctatca gctgagcggc gacaatccct acaacaatcc
      601 ggggttcgac ccgttgggct gtcagacgcc gtcgccgtca gggtgtccca acttcaaccc
      661 gcaaaatggc cttgagacca accgctcgga gtatgttgcg ggtctttacc tgtcgccgtt
      721 cgccggcttc aacgtggtgg cgcagggccg cttcgatgag agggactggt cgctgcgacg
      781 tcaggacctg ctggtcaacg ccaactatgg cccggtgatc gcctcggtcg cctactcgct
      841 cagccgcttc gatccatcga cggggaccca gagcacccag caagatgtca tgccctacct
      901 gggcttgaag attaccaaca attggagcat cggcggctcg atgcgctatg acatcgatgc
      961 caaggagcgc atccaggatc aactccagct caaatacgcc gacgaatgct ttgttctgac
     1021 ggcgagctac accgaaacct ttatcgaaaa cccatccctg gatctgcggc ccgatcgcac
     1081 gttgatgctg cgcttcgagc tcaagcatct gggtgagttc aactacaaga ccgacgtcct
     1141 gagccacgtg ttcggcgaca gcaaccaggg cccgcaatag cgccgcccgg cgtcctgagc
     1201 ccgggctttg gcgctcgccg cagggtgccg ctgccttgcc cacgccacgg ttgtctgtgg
     1261 ataaagagga tcgccgctga gggcgcggcg ctaggatgga ccatcgaatc gacgctcttt
     1321 tagtagcccg gcagccgacg cacatgcacc agcccgtttt tcgtcgatcc gttcccgctg
     1381 ccctgctggc atcggcactg ctgatgctgg gctccccgcc tctcagtgcc gcgcaaaccg
     1441 gcaaggcgca gaccgcggcg gtggaaaagc agggggccgc cgccgccagc aaggcgggcg
     1501 gccaaagcat tgtggcgctc gtcaacgacg agccggtgac ggcctatgag atcgagcagc
     1561 gcgcgcgctt cctgtcgctc agctccaaca tcggcgacaa ggtcaaggaa aacttcaagc
     1621 gtctggccga gcaggagagt accaaccagc agctgcgcgc catcatgcag caggtgatcg
     1681 acagcaacaa aggcaaggcg cccgatcagc tcagggccat tttcgaggag aagaagaagc
     1741 agttcagcat gaccctcgag cgccaggcca tcgagagcgc gcgctccggc ctcgtgccgc
     1801 aaatgcgcaa gggcgcccag gaggagctga tcgaggaacg cctcaagctg caggaagcca
     1861 aaaagaacgg cattgaagtc agcgacgaag aagtacgacg ggtgttcaag gggctggccg
     1921 acaacaacaa gatgaccgag gaacagttcg tccagcacct gaaggggctc ggcgtcgatg
     1981 ccaacaccct caaggagcgc tttcgcgcca tgatcggctg gcgcgaggtc attcgccgcc
     2041 gcttctcggc cctggtctcg gtgacccaac gcgaaatcga ccgcgccgtc tcggcctccg
     2101 gcgatggcgc cgatgatggc gtcgagttgc acgtgcagaa gatgaccctg tcgctgccga
     2161 ccgcctccga ccaggcccag atggccaagc gcttcgcgga agccgaggcg ctgcggcgca
     2221 agttcggcgg ctgcaagagc atggcggggc tcgccaagga ggcaccgggc gtcaaattcg
     2281 acgacatgaa attcatcaaa ccgagcagca ttagcgaacc gacgcgatcc ctgctgctca
     2341 gcgccaaaga cggcgatatg ctgccgccga gtgccacacc gggcgcaatc gacttgcttg
     2401 ccgtctgcgc ccggcgcacg gtcaagggcg acgagaagca gcgggagaag gtgcaggagg
     2461 atttgcgggc gaaggaattc gagatgctcg ccaagcgcca cctgcgcgat atcagacagg
     2521 atgcccacat cgaattcaga tgaggcacat gccgcggcgc cggcgcacgc cgcggccgcc
     2581 cctgagctgc cgccgctggc cgtcacgatg ggtgacccgg cgggaatcgg gccggacata
     2641 acgctcatca gttggctcaa tcgcgccgcg ctggcgctgc cgcccttcgt ccttttgggc
     2701 gatgccgacc tcgtggccga gcgggcctcg cagctcggcc tcgccatccc gatcgaacgg
     2761 gtgggcgagt ttgccgccgg ccgggaggcc ttcgcccgcg ccctgccggt gctgcccgtg
     2821 cgcccggccg gcgccgacca aagcccgatc gtggcgggga tcgaacaggc cgtgctggcc
     2881 gtgctggagg ggcgggcctt ggcgctggtg acaaaccccc tcaccaagag cgcgctcaat
     2941 ccggccgggc tctcctatcc cggccacacg gactacctgg cgctgctcgc ggaacgtttt
     3001 gccggcgggc cgcggccgcg gcccgtcatg atgctggccg ccgaggagct caaagttgtg
     3061 cctgcgaccg tgcacgtgcc gatcgctgcg gtccccacct tgctgacccg cgagcttctc
     3121 agcgagacca tcgccatcac ggccgccgcg ctcagccaag acttcggcct ggtggcgccc
     3181 cgcatcgccg tgacggggct caatccacac gccggcgaag acggcctgat cggcaaggag
     3241 gagcgcgagc tcatcggccc ggtgctggca agcctcgcgg cgagcgggct cggcgtcacg
     3301 ggcccgcatg ccgccgacac cctgttccac gctgcggccc gccgcagcta tgatgccgtg
     3361 gtggccatgt accatgacca ggcgctgatc cccctcaaga ccctggcctt cgatcgcggc
     3421 gtcaacatca cgctcggctt gcccttcgtg cgcacctctc ccgaccacgg taccgccttc
     3481 tcccttgccg gcacgggcaa ggcgcgcccg ggcagctttg ttgccgccgt gcggctcgcc
     3541 gccgagattg gcgcccgccg cagggccgcg ctcgggccac agcggcgatg acgcgcgttg
     3601 ccgccgcgac gcccgacggc ctgccgccgc tcggcgaggt gatccgcagc cttgagctcg
     3661 ccgcccgcaa aagcctcggg cagaacttca tcctcgatct caatttgacg cggcgcattg
     3721 cccgcgccgg cggcagcctc gctgggcgca gcgtggtgga ggtgggcccc ggcccgggcg
     3781 gcctcacgcg ggcgctgctc ttggagggtg ccagccgggt cgtggccatc gagcgcgacg
     3841 agcgctgcgg cccggcgctg gcggccatcg cgcagcgcta tcccggccgc ctcagcgtgc
     3901 agttcgagga tgcgctcaag accgactggt cgaaattggc tgcggcgctg gcgccggagc
     3961 ggccgctgat tgctgccaac ctgccctaca atgtggcgac gctgttgctg atcggctggc
     4021 tcgaaagcga gccgtggccc ccgtggtacc gcggcatggt gctgatgttt cagaaggagg
     4081 tcgcccagcg cattaccgcc ccggttggca ccaaggccta tggtcgtctg gccgtcattg
     4141 cgcagtggcg ctgccgcgtg cggttgctgt tcacgttgaa gccggaggcc ttcaccccgc
     4201 cgcccacggt cgcctccgcg ctcgtcgcct tcgagccggt cgaacgacca cagccggcct
     4261 gcagcgtggc gagcctcggc cgggtcacgg ccgcggcctt cggccagcgc cgcaagatgc
     4321 tgcgccagag cttaaagccg ctgctcgccg atacggaggc gctgctcggc gccgccggga
     4381 tcgcgccgac gctgcgcggg gaagcactca gcgtcagcga gttcgcgcgg ctcgcctacg
     4441 tgctggagcg ctggccgggg cgctaacgcg ccgctcatag ctgcccgagc aggcccgcaa
     4501 caaaccccgc aaggcccgtc tggcgcgcgc gctgcaagcg ttcggccgcc aggatcgcgc
     4561 gcaaatcggc gaggctcgtc gccagatcgg cgttgacgag cacatagtcg tagtcgcgcc
     4621 aatgcgcgat ttcgctggcg gcggcggcaa ggcgtcggct gaccaccgcc tggctatcct
     4681 gggcccgcgt ctgcaagcgg cgctccaagg tcgccccgtc cggcggcaaa atgaatacgc
     4741 caaccatatc ggcgccgagc tgggcgcgca gctggcgagc gccctgccaa tcgatgtcga
     4801 acagcatgtc ctcgccacgc gccagcgcgt cgaggaccgg tgccctcggc gtggcgtaaa
     4861 agttgccgtg cacctcggcc cattcgatca gctcgccggc ttgcttgcgg cgctcgaatt
     4921 ccggctgatc gatgaaacta tagtccttgc cgtcgcgctc gccctggcgc ggcgcccggg
     4981 tggtgaccga gaccgacatc ctgagcgtgg catcgctctt cagcaaaccg ctggcgagcg
     5041 tcgtcttgcc cgcgcccgac ggcgaggaga gcacgaggag cagcccgcgt cgttcgatgc
     5101 gccccgcctg tgggcccgcc tcactcaatg ttttgcacct gctctcgcat ctgatcgatc
     5161 accgctttca agtccagccc ggcgcgtgtg gtgtcctcgt cgctggcttt ggagcacagc
     5221 gtgttggcct cgcggttgag ctcctgcgcg agaaagtcga gccggcgtcc agccggggcg
     5281 tcgccggcca gcaattcgcg cgcggccgcc acatggctgg agatgcgctg cagctcctcc
     5341 tcgacgtcgg cgcgcgcggc cagcatcgcc gcctcctgat agagacgcgc ttcgtcgaaa
     5401 ttcacgttgc tctccagcag gcggccgacc tgctctttca gccgtgcgcg caccgcttcg
     5461 atcgagcgcg ccggcaggac ggcgatgcgc tgggtcaagc gctcgatgac ggcaagctga
     5521 tcgaggacga tctgctcgag ccgtccccct tcggcggtgc gcacggcaac gagttcgacg
     5581 agcgcccgct cgagatcgcg cagcatggcc tcgccgcgcg cgctcacctc ctcggtggtt
     5641 tcctccggtt cgatcagctc cagcacgccc ttgatgccga gcagcgcctc ggcccggggc
     5701 ggggcgacct cgaccatccc cttcagccgt tccagcgcca gcagcacctg ccgcagcgcc
     5761 gcctcgttga gccggatctc ggcgccccca tcgctgcgct tggcgctgag gttgacggca
     5821 atgctgccgc gcgcgagatg ctgcgttatc gccgcacgga tgcgcggttc gaggctctcc
     5881 aagcctggcg gcaagcgcag ccgcacgtcg agcccgcggc tattgaccga gcgcagctcc
     5941 caatgccagg cggcggggcc ggcggtcccg tcgcgtgcgg caaatccggt catgcttttg
     6001 aggctcattg cgccggtcag gtcgtttcgg ggtttcgcaa gcgccagcga ctcggggatt
     6061 ggcggaactc tagccgcagc gccgggccgc aacaagaacg ggccggccgc cgccctaacc
     6121 ctagcgccgc ttcgggtcgg tcgccgggtc tgcggtggtc gcggcgccgg cgggcgcctt
     6181 atcggctgcg gcctctggct tggcctccgc cttggcgcgc gctgcggcgc gttgcttgac
     6241 gcccggacgc agcttcgggg tcggctcctc cgccggcggt tccggcgccg ccttggcctt
     6301 caagtccttc tctaccaggc gccatttttg cacgttggcg ttgtggtctc tcagcgtctc
     6361 ggcaaaggca tgcccgccgc tgccgtcggc cacaaaataa agctcgcgcg tttcgcgcgg
     6421 ctgcagcacg gcttcgatcg cccgccgccc agggttgcag atcggcgtcg gcggcaggcc
     6481 gtcgatttga taggtgttgt ggggggtctt ctgggtgatc tcgttccttt ggatcgggcg
     6541 tccccaggca accttgcccc cgtacagccc gtagagaatg gtggggtccg actgcagacg
     6601 catgttctgg cgcagccggt tgatgaatac cgcggccacc cggtcgcgct cgtcgttgcg
     6661 cccggtctcc ttctcgacga tcgaggccag cgtcacggcc tcgtcccagg ttttgaccgg
     6721 caggtccttt ttacgctgcg cccacatccg ctccatcagc ttcttcgatt ccgccgccat
     6781 gctctcgagt acggcctcgc gctgggcgcc acgctggatc acgaaggttt ccggcatcag
     6841 gctgccctcg gccggcgtcg ccgagatctc accgctgaga ttggcgtcgg ccagcagccg
     6901 atcgacgatc tgctggctcg tcagcccctc gggcacggtg atcttgtagg tcaccgtctt
     6961 gccgtcgcaa atgagatcga tcagctcgcg cacgctggcg tgcgggcgca cctcatagtc
     7021 gccagccttg agttgtaggg tcttgtcacc ctcgagcctg gccagcagct gcagccagcg
     7081 gtagccggcg gtgaacaagc gccggtcgct gatgacgccc tcgcgttcca gacgcgccgc
     7141 gatctcgagc gaaccctcga gcttgggaat gacgatgacc ttgtttctct ccagcgggcc
     7201 cggcgcatcc atctgcccat cgaagtagta ggcgagcgcg cccagcagca gcatgccgac
     7261 cagcgcgacg gtcaacagcc cattcataaa agccagcacg ggccggggcc ggtgctcttc
     7321 ctcgagccgg attttgcggt tgacccgtgc cggccgcgtc ggctcgagcc gttcagcggg
     7381 gctacgcgcg agcgaaccgc gcgtgcggga ggtcttgggg tctgcgtgtc ggtccgccgg
     7441 gtggcggttt ctcatggctc acgttccccg tcagatgcgc tgccttttgc aagccttgcg
     7501 gcgcgctcca acgcccacgc attcggcacc cgaacctagc agcaaatgtg gcgaaagcag
     7561 gattgcttgg caagcgcgga cgtggcggca aggttagggc gcgcgcgggc cacagcgcgg
     7621 ccctgcgccg cggctgtggc ggcttggcgt caggcggcgt ggcaggaggg cgtcacgtca
     7681 aggcatcggc cctcccgcgc cgctgccgcg gcgcaccccg cggcgcttgc gcgcgctcct
     7741 ttggggcctc gcagcgcgct ggccctgcag ggcaaggcag cgcggcctgc cggtggcgcc
     7801 cgccgcctat gccttgaagc gctgcaacac cagcgaggcg ttggtgccac cgaagccaaa
     7861 tgaattcgac agcgcggtat cgatccggcg cgcttgcgcc ttatgcggca ccaagttcac
     7921 cggggtgtcg accgagggat gatcgagatt gagggtcggc ggggcgatat tgtcgcggat
     7981 cgccagcacc gagaagatcg cctccaccgc gccggccgcg cccaacaggt ggccgattgc
     8041 cgacttggtc gatgacatgg cgagtttgcc ggccgcattg ccgaacaggc gctcgacggc
     8101 ggcgagctcg atctcgtcgc ccatggtcga ggtgccgtgc gcattgatgt agtgcacctc
     8161 gctcggctcc acgcccgcgc gctgtagcgc catgcgcatg gaccggtagg cgccgtcgcc
     8221 atcctcggat ggcgcggtga tgtgataggc gtcgccggac aagccgtagc ccacgacctc
     8281 ggcgtagatc tgcgcgccgc gtgccttggc gtgctcgagc tcctccagca cgacgacgcc
     8341 ggcgccctcg cccatgacga agccgtcgcg atccttgtca tagggacgcg aggccttttg
     8401 cggttgatcg ttgaaggtcg tgcacagcgc gcggcaggcg gcgaagccgg cgatcgctat
     8461 acggcaaatc gggctttcgg cgccacccgc gagcatgacg tcggcatcat cgagcgcgat
     8521 catccgggcg gcatcgccga tcgcgtgcgc cccggtcgag caggccgtga ccacggcatg
     8581 gttcggaccc ttgagggcat gtttgatcga gacatagccg gaggcgagat tgatcaagcg
     8641 tcccggaatg aaaaacgggc tgacgcgccg tggtcccttc tccttcagaa gaatggaggt
     8701 ttcggcgatg ccgttgaggc caccgatacc ggcgccgatc agcaccccgg tgcgctcctg
     8761 cttctcggtc gtgtccgcgc gccagccggc gtcctcgagg gcctgcgtcg cagcggccat
     8821 gccgtagata ataaagtcgt cgaccttgcg ctgctccttg ggctccatcc agtcgtcagg
     8881 attgaacttg ccctccgaca gggggccgcg cggaatgaaa cacgccacct ggcagctaag
     8941 atcgctgacc tcgaattctt cgacgcggcg cgccccgctc tctcccgcga tcaggcgctt
     9001 ccagcttgcc tcgacgccac agccaagggg tgtcaccaag ccgaggccgg tgatgactac
     9061 gcggcggggc attcgcatgc tcaacatcgt cggaataggg cgagggctcg atggtcgtgg
     9121 cgccagccgt gctttgcgaa aacgctgcgc gcccgcgatc caaaaaggct acgcggcgct
     9181 cgcgtgcttc tcgaggaatt tgacggcatc gccgacggtt tgaatctgct cggcggcgtc
     9241 atcggggatc tcgcagccga actcctcctc gaaggccatg accagctcga ccgtatcgag
     9301 gctgtcggcc cccaggtcat cgatgaaact ggcgctgtcg gtgaccttgt cggcctcgac
     9361 accgaggtgc tccaccacga tcttcttcac gcgcgctgcg acgtcgctca ttgctctatc
     9421 ccttggctct ttgctgatcc acgctgttgc cgtgtgccaa aaaccgggcc tggtcaagcc
     9481 cacgcccgaa actgcgccat ggct
//
LOCUS       DASTMT010000203         6201 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_1746721, whole genome shotgun sequence.
ACCESSION   DASTMT010000203 DASTMT010000000
VERSION     DASTMT010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 6201)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6201)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6201
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1746721"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..396)
                     /locus_tag="VFR00_04715"
     CDS             complement(<1..396)
                     /locus_tag="VFR00_04715"
                     /inference="COORDINATES: protein motif:HMM:NF015810.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEU0158588.1"
                     /translation="MQCKEAHETLSALIDDELGSEQQHAAKAHIAGCSLCAEVARDYR
                     RIGHDLRAAARVPLPADLAHKVRARLAAEARSGHRPQRLPWQWLVQYAASLILVAGLS
                     GLLGWQVARTSFEQRRLERDVVAAHVRSLL"
     gene            complement(386..940)
                     /locus_tag="VFR00_04720"
     CDS             complement(386..940)
                     /locus_tag="VFR00_04720"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU0158589.1"
                     /translation="MVAKTPFEELLTPHLQAAFNLAYWIVRSREEAEDVVQDAYVRAF
                     RAFSSFRGAAPKPWLLAIVRNVAYRALQNRKRAGNVIFFSEDLKHRERDAISEAPSSE
                     PSAESLLVAADEKQQLLAALATLSVDYREVIVLRELEGLSYNEIAEAISAPVGTVMSR
                     LSRARAELRAQLARRIAKDEPNAV"
     gene            1392..1580
                     /locus_tag="VFR00_04725"
     CDS             1392..1580
                     /locus_tag="VFR00_04725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0158590.1"
                     /translation="MLRQNADPSERDRAYEALSILALLLCLVGVLMYASLSSTTVVVA
                     APPSLDALNGAADRAPAP"
     gene            1765..2673
                     /locus_tag="VFR00_04730"
     CDS             1765..2673
                     /locus_tag="VFR00_04730"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU0158591.1"
                     /translation="MADTGSGPDQPQGTIGIIGLGIMGGAMAANLVRGGWRVIGYDID
                     ATAREAARQSGVEVVDGAAALAAATSDIILSLPSGQAAIASAETIAAAAPSRAVVLEM
                     STLSLEDKHAVARALASHGHIALDCPLSGTGAQAQSKDLVVYASGHGPDIERLVPLFL
                     GLGRRYFNLGDYGNATKMKFVANLLVAIHNVASAEAMVLGMKAGLAPEQIVEVIAAGA
                     GTSRVFELRAPLMAENRYEPASMRCSTWQKDMAVIGAYAAQLGCPTPLFSASDAIYRA
                     GLARGMGAQDTASVCAVLEAMAGIRR"
     gene            complement(2734..3363)
                     /locus_tag="VFR00_04735"
     CDS             complement(2734..3363)
                     /locus_tag="VFR00_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009489697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU0158592.1"
                     /translation="MARILIVDDQYLVRLGLRRILRAHPSLKVVGEAADGEEAVAQAL
                     ETNPDVILLEYLLPLITGLEVVRRVRAHHPHTEFLMFTLHDNEVLIEQALKAGVRGYL
                     LKSDPSHELITAITALAAHQPYFTSKVSLLLLEALLATPDEAAIITNRQRCIVQLIAE
                     GYQTKQIAKILGIGLKTVETHRAHIMHRLDLSSSAALVRYAIRSRLAEA"
     gene            complement(3573..3809)
                     /locus_tag="VFR00_04740"
     CDS             complement(3573..3809)
                     /locus_tag="VFR00_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012330767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0158593.1"
                     /translation="MPELDRRTLLAAMLAGIAVVGSASTSVAATTIAAPPPGLIPRTG
                     LQPLHKVQVVVGPRRRRRWRCWWRRHRRVCGWRW"
     gene            4233..5027
                     /locus_tag="VFR00_04745"
     CDS             4233..5027
                     /locus_tag="VFR00_04745"
                     /inference="COORDINATES: protein motif:HMM:NF021589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEU0158594.1"
                     /translation="MRKLNALAGAVGLLGMQQALAGEESAPPALQRPTWSVFVSIAPT
                     YSTNALARESDRRRDVYAQPDVMLRLDGKLASDLSYRLYGRTSFNSFSEESFANETFA
                     LFGARLIKTLGDWRTSVSYEHRFEYSGVYSDLSFIADDLQISTTRDFNWGPFTVSPIA
                     QFGYRFADVAEARRYRLDLLLPIEAKLISRWSIVSTPLLEAYWYTGGLNDGRQDIVYS
                     ASLGLRYSIASNMSLTTSANYEWRTSNVPLRNYRDLEIGPRLDMAF"
     gene            5045..5251
                     /locus_tag="VFR00_04750"
     CDS             5045..5251
                     /locus_tag="VFR00_04750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0158595.1"
                     /translation="MQPIFILFAVAGALLATASPALAQRSEVGDRGVRLETNALRTDV
                     RRERLKKPNRVSVVPKAKRRVKVD"
     gene            complement(5335..6138)
                     /locus_tag="VFR00_04755"
     CDS             complement(5335..6138)
                     /locus_tag="VFR00_04755"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEU0158596.1"
                     /translation="MTYATVKLEHDGPLAVLTLNRPEKMNSLSDQLLADFRAAMDTCE
                     ADESVRAMIVTGSGKAFSAGFDIAPRAKPRTTVQDWRDHAKDGNETWLRVWRSRLPVV
                     AAVNGYCLGGGCDLSMTCDYTVAADTAQFGEPEIEFCSAPPFLIMPWVLAMKHAKELL
                     LLGERIDAAEAFRMGLVNRVVPADTLMAEAKKIALRLARLPAIAVKQNKEAVNRAYDM
                     RGLVATLEYGQEMFCMTAMAHSPEGQEFRSIAREQGLKAALRWRENRFK"
BASE COUNT         1018 a         1982 c         2048 g         1153 t
ORIGIN      
        1 cagcaacgag cgcacgtggg cggcgacgac gtcgcgctcc agacgccgct gctcgaagga
       61 cgtgcgcgcc acctgccagc cgagcaggcc tgaaagaccc gcgacgagga taaggctggc
      121 ggcatattgg accagccatt gccaggggag gcgctggggc cgatggcccg accgtgcctc
      181 tgccgcgagc cgggcacgca ccttatgggc gagatcggcc gggagaggga cgcgcgccgc
      241 ggcccgcaag tcatggccga tgcggcggta gtcacgcgcg acctccgcgc acagcgagca
      301 cccggcgatg tgggccttgg cggcgtgctg ctgctcgctg ccaagctcgt cgtcgatcag
      361 cgcgctgagc gtctcgtgcg cctctttaca ctgcattggg ctcgtccttg gcgatgcgcc
      421 gtgccaattg tgcgcgaagc tccgcgcggg cccgcgagag gcgtgacatg acggtgccga
      481 cgggtgcgct gatcgcctcg gcgatctcgt tgtagctcag gccctccaat tcgcgcagaa
      541 cgatcacctc acgatagtcc accgacagcg ttgccagcgc cgccagcagc tgctgttttt
      601 cgtccgcggc caccaggagg ctctctgccg atggctccga cgatggggcc tcgctgatcg
      661 catcccgctc ccggtgcttg agatcttcgc tgaaaaaaat cacgttcccg gcccgcttgc
      721 gattttgcaa ggctcggtag gcgacgttgc gcacaatggc cagcagccat ggcttgggcg
      781 cagcgccgcg gaagctcgag aaggcccgga aagcgcgcac ataggcgtcc tggacaacat
      841 cctcggcctc ttcccgacta cgcacgatcc agtaggcaag gttaaaggct gcctgcaagt
      901 ggggcgtcag cagttcttcg aatggcgttt tggcgaccat tcccgtcctc agcagccggg
      961 tatggtccat taaaccgtag atcaggacag cagggaattt attcccgcca cagcaaaaat
     1021 aattgatcga ttcggcagcc gttccgggaa tgcggcgccg ggctccggag tctgggtctt
     1081 aggagccgtt ttgcgcagcg ggggcgggcc cgcgcgcgtt ccttccgctg cgcccgccct
     1141 gagccagaaa gctggaggcg ccggtatggg tgctcgcctg caagacgtca gggtttgcca
     1201 tcccgagcgg ccgcggcggc gaacgcttgc ggcacgatgc aacggggcgg ccccctccgg
     1261 ccccgcgttc agctggcgtg gtcccggcgt gcaagccccg tcggggcccc ccgagtgatg
     1321 ggatcgccag atgctgccgg agccactgct gatccgatgc agcaaacgac caattgctgt
     1381 tggaggattt gatgttacgt caaaatgcgg accccagcga gcgcgaccgc gcctatgaag
     1441 ctttgtccat actggccctc ttgctttgcc tggtcggcgt cctgatgtat gcgagcttgt
     1501 cgagcacaac cgtcgtggtg gcggccccac cgagcctcga cgcgctcaat ggcgctgcgg
     1561 accgcgcgcc ggcgccttaa tgctttaaga acccccatta ccctaagcgc ggcggggttg
     1621 gccgcgctcg gcctcggaca tccccctttg cagcgcgcgc ttgtgctttg gcgcctcgtt
     1681 ccgagcgggg tctgtccagg ctttgttgcc atcgctacca tgcgccttgg gaggccttga
     1741 gacgagggcg acgaggagac gaggatggcc gacacaggca gtgggcccga tcagccccag
     1801 ggcacgatcg ggatcatcgg tcttggcatt atgggcggcg ccatggccgc taatctcgtg
     1861 cggggtggct ggcgggtgat cggttacgat atcgacgcca cggcgcgaga ggcggctcgc
     1921 cagagcgggg tcgaggtggt cgacggcgcg gcagcgctcg cagcggcgac gagcgacatc
     1981 atcctctcgt tgccgagtgg gcaggcggcg atcgcgagcg cggagacgat tgccgcggcg
     2041 gcaccctccc gcgccgtggt gttggagatg agcacgcttt ccctcgagga caaacatgcg
     2101 gtcgcgcgcg ccttggccag ccacggtcac atcgccttgg actgcccgct gtccggcacc
     2161 ggcgcccagg cccaaagcaa ggacctcgtc gtctacgcca gtggccacgg ccccgacatc
     2221 gagcgcttgg tcccgctgtt cctggggctc gggcgccggt atttcaatct gggcgactac
     2281 ggcaatgcga cgaaaatgaa attcgtcgcc aacctgctgg ttgccattca taacgtcgcc
     2341 agcgccgagg ccatggtgct cggcatgaag gccggtctgg cgccggagca gatcgtagag
     2401 gtcatcgcgg cgggcgcggg aacttcgcgt gtcttcgagt tgcgggcgcc gttgatggcc
     2461 gagaaccgct acgagcccgc cagcatgcgt tgctcgactt ggcaaaaaga catggccgtg
     2521 atcggtgcct atgccgcgca gctcggctgc ccgaccccgc tctttagcgc cagcgatgcc
     2581 atctaccgcg ccggtctggc gcgcggaatg ggggcgcagg acaccgcctc tgtgtgcgcg
     2641 gtgctcgagg ctatggccgg catcaggcgc tgaccgtcag cgccgccttt gccggcgcac
     2701 gctgaggttc tgccgcggcc cggccgaggg cgctcaggct tccgccagcc tggaacggat
     2761 cgcatagcgc acgagcgccg ccgaggagct caggtcgagc cgatgcataa tgtgggcgcg
     2821 gtgcgtttcc acggtcttga ggccgatgcc taggattttg gcgatctgct tggtctgata
     2881 gccctcggcg atcagctgca caatgcagcg ctggcggttg gtgatgatcg cagcttcgtc
     2941 gggggtggcc agcagggcct cgagcaggag gagggagacc ttgctcgtaa agtacggctg
     3001 atgcgcggca agtgcggtga tggcggtgat cagctcgtgg ctgggatccg acttcagaag
     3061 atagccgcgc acgccggcct tgagcgcctg ctcgatcagg acttcgttgt catgcagcgt
     3121 gaacatcaag aactcggtgt gcgggtgatg cgcccgaacg cggcgcacga cctccaaacc
     3181 ggtgatgagc ggcaacaggt attcgaggag gatgacgtcg gggttggttt ccagggcttg
     3241 ggcgacggcc tcttcgccat cggcggcttc gccgaccacc ttcaggcttg ggtgggcgcg
     3301 cagaattctg cgcagtccga gccggacgag gtactgatca tcgacaatca aaatgcgtgc
     3361 catgacacgc gctgatctct cacccccttt cgcttcgact gcacaattct cgcgcgcttt
     3421 gccggcgctg cccatcaaga caaacccgga aaagggccgg gcggtcacgc agtcttggcg
     3481 gcggcggctg cctaagcgcc ccgtcgcgtt gtgcaggcgc gaaagtcgca cggggacttc
     3541 actggccaaa agcgtccgct ggcgggggtc acctaccagc gccagccgca gacgcgacga
     3601 tggcgcctcc accagcagcg ccaacggcgc cggcggcgcg gtccgaccac cacctggacc
     3661 ttgtgcagcg gttgcaaacc cgtgcgcggg atgaggccgg gcggcggcgc agcaatcgtt
     3721 gttgctgcaa ccgacgtgga tgcgctgccg acaacggcga tcccggccag catggcggcg
     3781 aggagggttc ggcgatcgag ctcgggcatg gctttacgct cctgacttag gtggtcccgc
     3841 accgagggcc ggcgtcgtcg ctccgacgtt gacgcccttc gcactcatgc gcgcgtcgcg
     3901 ccgcgctgta catggtgggg aaccctgaaa gttgcgcgct tcgtctcaac gtcgtggcgc
     3961 ccgcagcaga gcgcaacggc gcagcgggcg cgtcggcagc ggtgagggcg gaaaccagcc
     4021 tggtttccag gccgcgaata gcttaaggtc caagcccgtt gttttgagtc gcttcctctt
     4081 catgttgtta gcggcatccc gttgtcgatg ccgcagctgt cattggttgc ctgtccgacc
     4141 ccgttgcagc gccttttcgc tgtttcctgc gcgtttttcg tgatccgcag tcgatcctgt
     4201 ttttccccga accgcttcga gggttcactt tggtgcggaa gctgaatgct ttggcgggtg
     4261 ccgttgggct gctcgggatg cagcaggcgc tggccggtga ggagagcgcg ccgccggcct
     4321 tgcaacggcc gacctggagc gtgttcgtct ccattgcccc aacctacagc accaacgcgc
     4381 tggccaggga aagcgaccgt cggcgggacg tttatgccca gcctgacgtg atgctgcgcc
     4441 tggatggaaa gctagcgtcg gatctttcct atcgcctcta cgggcgcacc tccttcaatt
     4501 cctttagcga ggaaagtttc gccaacgaaa ccttcgccct ctttggcgcg cgcctcatta
     4561 agaccctggg ggattggcga accagcgtca gctatgagca ccgtttcgaa tactccggtg
     4621 tctacagtga cctgtcgttc atcgccgatg atctgcagat ttccacgacg cgcgacttca
     4681 actgggggcc attcacggtg tcgccgatcg cgcaattcgg ttaccgcttc gccgatgttg
     4741 ccgaggcgcg gcgctatcgg ctcgatcttc tgttgccgat cgaggccaaa ttgatctcgc
     4801 gctggtcgat cgtttcgacg cccttgctcg aagcctattg gtacaccggc ggcctcaacg
     4861 atggacgcca ggacatcgtt tattccgctt cgcttggttt gcgctacagt attgccagca
     4921 acatgtcgct gacgaccagc gccaactatg aatggcgcac ctcgaacgtt ccgctgagga
     4981 actatcgcga tctggaaatc ggaccgcgac tcgatatggc cttctagtgc tctgggagcc
     5041 cagcatgcag ccgatcttca tcctcttcgc cgtggccggc gccctgctgg cgacggccag
     5101 cccggccctg gcgcagcgca gcgaggtcgg cgaccgtggc gtgcgcttgg aaaccaatgc
     5161 gctgcgcacc gacgttcgcc gcgaacgcct gaaaaagccc aatcgggtga gcgtcgtgcc
     5221 gaaagccaag cggcgcgtca aagtcgactg agggcctgcc cggggtcgct tgcgccctgg
     5281 cgtaggccac agctcttctc ggctcgggtg cgtcgggccg gcgccatctg tgcgctactt
     5341 gaaacgattt tcccgccagc gcagcgccgc tttcaacccc tgctcgcgcg cgatgctgcg
     5401 gaattcttgg ccctcggggg agtgcgccat ggccgtcatg cagaacattt cctggccata
     5461 ctcgagcgtg gcgaccagcc cgcgcatgtc ataagcgcga ttgacggcct ccttgttctg
     5521 tttcaccgcg attgcgggca gccgggccag ccgcagcgct attttttttg cttccgccat
     5581 gagcgtgtcc gccggcacaa cccggttgac gagacccata cggaaggcct ccgccgcgtc
     5641 gatgcgctcg cccagcaaaa gcagctcctt ggcatgcttc attgccagca cccagggcat
     5701 gatgagaaag gggggcgctg aacagaattc gatctcgggc tcgccgaatt gggcggtatc
     5761 ggcggccacg gtgtagtcgc aggtcatcga caggtcgcag ccgccaccga ggcaatagcc
     5821 gttcaccgcc gcaacaaccg gcaagcgcga acgccagaca cgcagccagg tttcgttgcc
     5881 gtccttggca tgatcacgcc agtcctgcac cgtcgtgcgc ggttttgcgc gcggggcaat
     5941 gtcgaagccg gcgctgaacg ccttgcccga gccggtcacg atcatggcgc ggacgctctc
     6001 gtcggcctcg caggtgtcca tggcggctcg aaaatcggcg agcagctgat cggagagcga
     6061 attcatcttc tccggccgat tgagggtcag caccgcgaga gggccgtcat gttcgagttt
     6121 tacggtggcg taggtcatgg cgttccctgg ctggttggtt tgcgagcaat cacgagggca
     6181 tcgaggacaa gcgcaccctg g
//
LOCUS       DASTMT010000204         4375 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_6291393, whole genome shotgun sequence.
ACCESSION   DASTMT010000204 DASTMT010000000
VERSION     DASTMT010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4375)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4375)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4375
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6291393"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            368..1723
                     /gene="glmU"
                     /locus_tag="VFR00_04760"
     CDS             368..1723
                     /gene="glmU"
                     /locus_tag="VFR00_04760"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEU0158597.1"
                     /translation="MTSPPLLIVILAAGHGVRMRSQRAKVLHRLAGRSLLGHALAYAQ
                     AAGATALAVVVPPGSEAVRAEVLAQAPAAALFEQSRQAGTADAVLAAREALSRHAGDV
                     LVLFADTPLVEVATLERLRAALHQGANVAVLGFEPDEPAGYGRLILDEAGRVRAIREH
                     KDASAAERSQRLCNAGAMAFRVPSLLDLLGRIGNDNASREYYLTDAVALANAEGLIVL
                     PVLCAPEEAMGINTREQLAAAEAIFQRRARQKAMREGVSLIAPETVWFSYDTKIARDV
                     TIEPNVFFGPGVTIAEGAEILGNSHIVGARIAAGARVGPFARLRPGAEIGDGVHVGNF
                     VEVKNASLEAGAKANHLAYIGDGRVGAGANIGAGAIFCNYDGFNKNFTDVGAGAFIGS
                     NAALIAPVRIGEGAYVASGSVISRNVEADALAIARGVQEARPGWAAKFRALMQNRRKK
                     P"
     gene            1824..3644
                     /gene="glmS"
                     /locus_tag="VFR00_04765"
     CDS             1824..3644
                     /gene="glmS"
                     /locus_tag="VFR00_04765"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014495162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEU0158598.1"
                     /translation="MCGIVGILGQRPVALDLVDALRRLEYRGYDSAGVATVENGHLER
                     RRAEGKLKNLEQRLSQQPLNGHAGIGHTRWATHGRPVERNAHPHMNDRVAVVHNGIIE
                     NFQELREELSGKGHRFETDTDTETVLHLVTDYLDQGLAPVAAAQAALGRLRGAFALAL
                     IFAGDDNLMIGARQGPPLAIGYGTGEMYLGSDAIALAPFTDTITYLEDGDCAVVTRHT
                     AEIFDPAGRRIQRPQIRSVASSLLIDKGNFRHFMAKEIHEQPEVISHTLAHYVDFASG
                     RVLLPDFNLDPASISEVVISACGTAYYAGLIGKYWLERYARIPVEIDIASEFRYREPP
                     LRQGGLALFITQSGETADTLATLRYCKAQGQPIAAIVNVRTSTIARESDVVLPTLAGP
                     EIGVASTKAFTCQLSALASLTIALGRARNTLSAAGERDLVQALSQVPRHVSTLLRNET
                     QFERLAHELAKARDVLYLGRGVSYPLALEGALKLKEISYIHAEGYAAGELKHGPIALV
                     DENVPVIVVAPEDELFEKTVSNVQEVAARGGKIILISDADPARAGCKLEAHIPVPKAH
                     AFATPLIYAVPVQLIAYHTATFMGTDVDQPRNLAKSVTVE"
     gene            3843..>4375
                     /locus_tag="VFR00_04770"
     CDS             3843..>4375
                     /locus_tag="VFR00_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU0158599.1"
                     /translation="MAGVGLTLCAFGTCGFAAGKAADEAPAEAGEAGKPAGPAKKKSD
                     PVEAQRAVDNAGKLIEGGKTEQAIQVLGGALAAGNLPPAIMAKALLYRGIAYRQQKKP
                     AQAISDFTSALWLKAGLSEADRAEGLRQRAAAYAEAGLGQAGEAVPAAAKDTRSKERT
                     ASPAPWSDGTSTSGPPA"
BASE COUNT          764 a         1458 c         1455 g          698 t
ORIGIN      
        1 caaacgaaca aataggcctt aatgaagcgc tcggaccgag gggggtgagc agcgtgttct
       61 gccatggcca gcagcgtcct cgtgatggga gtgggggtgc ggcttggacg ctgaacaggg
      121 caccgcccga atcgggtaca aacgtgtcaa atggcagggg gattggtcaa cgcgcccggc
      181 ccgcgccaac cccgcaggct gtggacatgt gatggaggtg ccatagcgct ctgttgcaca
      241 taagacccaa tgggacacgg cacggggcgc cactatatgc tttttgcgtg acgcggcgcg
      301 ggggacgcca gcgtttcccc aagcggttgg cccaggcctc cgacctttgc aacctaagat
      361 accccagatg acctctccac cgctcctgat cgtgatcctc gccgctggcc atggggtgcg
      421 catgcgctcg caacgggcca aggtcctgca ccgcctggcc ggacgctcgc tgctgggaca
      481 cgccctcgct tatgcccagg ccgccggcgc cacggcgctt gcggtggtcg tgcccccggg
      541 cagcgaggcg gtgcgcgccg aggttctggc gcaggccccg gcggccgccc tgttcgaaca
      601 gtcgcggcag gccggtaccg ccgacgctgt gctggccgcg cgcgaggcct tgtcgcggca
      661 tgcgggcgat gtgctggtgc tgtttgccga tacgccgctc gtcgaggtgg cgacactcga
      721 gcgcctacgt gccgccttgc accagggcgc caacgtcgcg gtcttgggct tcgagcccga
      781 cgagcccgcc ggctatggcc gcctgatcct ggacgaggct ggacgcgtgc gcgctattcg
      841 cgagcacaag gatgcctccg ccgcggagcg cagccagcgc ctgtgcaacg cgggagccat
      901 ggccttccgc gtcccctcgc tcctcgacct cctcggccgc atcggcaacg acaatgccag
      961 ccgcgaatac tacctgaccg acgctgtcgc cctcgccaac gccgagggcc tcatcgtgct
     1021 gccagtgctg tgcgcacccg aggaggcgat gggtatcaat acgcgcgaac agctggccgc
     1081 agcggaagcg atctttcagc gccgcgcgcg ccaaaaagcc atgcgcgagg gggtcagcct
     1141 gatcgcgccg gaaacggtct ggttcagcta cgacaccaag atcgcgcgcg acgtcaccat
     1201 cgaacccaac gtcttcttcg gtcccggcgt caccattgcg gaaggggccg agatcctggg
     1261 caacagtcac attgtcgggg cccgcatcgc cgccggggct cgggtcggtc cctttgcgcg
     1321 cctgcgtcct ggcgccgaga tcggcgatgg cgtgcacgtc ggcaatttcg tggaagtcaa
     1381 gaatgccagt ttggaggccg gcgccaaggc caatcatctc gcctacatcg gcgatggtcg
     1441 tgtcggcgcg ggcgccaaca tcggcgccgg cgccatcttt tgcaactacg acggtttcaa
     1501 caagaacttt accgatgtcg gcgccggcgc cttcatcggc tcgaacgcgg ccctcattgc
     1561 acccgtccga attggtgagg gggcctacgt cgcctcgggc agcgtcattt cccgcaacgt
     1621 cgaggccgac gccttggcga tcgcccgtgg cgtgcaggag gcgcggccgg gctgggccgc
     1681 caaattcagg gctctcatgc aaaaccgcag gaaaaaaccc tgagaggggc accggccgaa
     1741 aaagcacgaa acattgtttg tattgaaact attgacgaaa tcggactagc aaggcgaaac
     1801 gcgacgagcc tgaggcagaa cacatgtgcg gcattgttgg aatactgggc cagcggcccg
     1861 tggcgctcga tctggtcgat gcgctgcgcc ggcttgaata ccggggctat gactccgcgg
     1921 gggtggccac cgtggaaaac ggccatctcg agcgccggcg cgccgagggc aagctcaaga
     1981 acttggagca gcgcctgtcc cagcagcccc tcaacggcca tgccggcatc ggccacacgc
     2041 gctgggcaac gcacggccgg ccggtggagc gcaacgcgca tccgcacatg aacgaccgcg
     2101 tcgcggtcgt gcacaatggc attatcgaga acttccagga gctgcgcgag gagctgagcg
     2161 gcaagggcca ccgcttcgag accgataccg acacggagac ggtcttgcat ctcgtcaccg
     2221 actacctgga tcagggcttg gcgccggtgg ccgcggcgca ggccgcgctc ggccggctgc
     2281 ggggcgcctt tgcgctggcc ctgatctttg ccggcgacga caacctaatg atcggcgccc
     2341 ggcagggccc accgctggcg atcggctacg gcacgggtga gatgtatctg ggctccgatg
     2401 ccatcgcgct cgccccgttc accgacacca tcacgtatct cgaggatggc gactgcgccg
     2461 tcgttacgcg ccacaccgcg gagatcttcg atcccgccgg gcggcgcatc caacggcctc
     2521 agatccgctc ggtggccagc tcgcttttga tcgacaaggg gaatttccgc cacttcatgg
     2581 cgaaagagat tcacgagcag cccgaggtga tttcgcacac gctcgcgcat tatgtcgatt
     2641 ttgccagcgg acgcgtgctg ctgcccgatt tcaatctcga ccccgccagc atcagcgagg
     2701 tcgtgatctc ggcctgcggc acggcctact atgccggctt gatcggcaaa tactggctcg
     2761 agcggtatgc gcgcattccc gtggagatcg atatcgcttc ggagttccgc taccgcgagc
     2821 cgccgctgcg ccagggcggg ttggcgctgt tcatcaccca gtccggcgag accgcggaca
     2881 cgctggcgac gctgcgctat tgcaaagccc agggccagcc gatcgccgcg atcgtcaacg
     2941 tgcgcacctc cacgatcgcg cgcgaatccg acgtggtgct gccgacgttg gcgggccccg
     3001 aaatcggcgt cgcgtccacc aaggccttca cctgccaact gagcgcgctc gcgagcctga
     3061 cgattgcgct cggccgggcg cgcaatacgc tgagcgctgc cggcgagcgc gacctggtgc
     3121 aggcgctgtc ccaggtgccg cgccatgtct ccacgctgtt gcgcaacgag acgcagtttg
     3181 agcgcttggc ccacgagctg gccaaggcgc gcgacgtgct ctatctcggc cgcggcgtca
     3241 gctatccgct cgccctggag ggcgccctaa agttgaagga aatctcctac attcacgccg
     3301 agggttatgc cgccggcgag ctcaagcatg gaccgatcgc cctggtcgac gagaacgttc
     3361 ccgtcattgt ggtggcgccc gaggacgagc tgttcgaaaa gaccgtgtcc aacgtgcagg
     3421 aggtggccgc gcgcggcggc aagatcattc tcatctccga tgccgacccg gcgcgcgccg
     3481 gctgcaagct cgaggcccac atccccgtgc ccaaggccca tgcttttgcc acaccgctga
     3541 tctatgccgt gccggtgcag ctcatcgcct accatacggc caccttcatg ggcaccgacg
     3601 tcgatcagcc gcgcaacctg gcgaaatcgg tgaccgtgga ataggtcggc gtggaacaga
     3661 gtgactgtgg aatagaagga taggcgaaaa gggagatttg cctcgagcct tgaccttgac
     3721 gtaggctcgc acggtggagg tgcttcgcgt aaggcttggg ggacggggac ggcataatgg
     3781 ctcgaggcat acgggctcga gacttgaccc gaagctggcg tttgcgaatc ctggcgccgg
     3841 tgatggcggg cgtgggcctg accctttgtg ccttcggcac ctgcgggttc gccgccggca
     3901 aggcggccga tgaggcgccg gccgaggccg gcgaggcagg caaaccggca ggtcccgcca
     3961 agaagaaaag cgatcctgtc gaggcgcagc gcgccgtcga caatgccggc aaattgatcg
     4021 agggcggcaa gaccgagcag gccatccaag ttttgggcgg cgcgctggcg gccggcaatc
     4081 tgccgcccgc catcatggcc aaggccctgc tctatcgcgg catcgcctat cggcagcaga
     4141 aaaaacccgc gcaggcgatc tccgatttca cgagcgcact ttggctgaag gccggtctca
     4201 gcgaggccga tcgcgccgag ggcctgcgcc agcgtgcggc ggcctatgcc gaagcgggtc
     4261 tcggccaagc cggcgaggcc gtcccggccg cggccaagga cacgcgcagc aaggagcgca
     4321 cggcgagccc cgcgccctgg agcgacggca cgagcacgtc cggcccgccc gccca
//
LOCUS       DASTMT010000205         7395 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_4322352, whole genome shotgun sequence.
ACCESSION   DASTMT010000205 DASTMT010000000
VERSION     DASTMT010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 7395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7395
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4322352"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..236)
                     /locus_tag="VFR00_04775"
     CDS             complement(<1..236)
                     /locus_tag="VFR00_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019404394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEU0158600.1"
                     /translation="MAMAERAGNAANADRSATAIAVLRQRFGERLQTGEALRRQHANT
                     LTWIPNQPPDAVIWVETTQEVREVVRVAATHGTP"
     gene            445..669
                     /locus_tag="VFR00_04780"
     CDS             445..669
                     /locus_tag="VFR00_04780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0158601.1"
                     /translation="MLNKILSFARSAAHNQNSPVFGASRGDTVSAENVRALATGAALA
                     VALDASPAVLVSIYDYAHRFRDHGARRLAC"
     gene            1050..2015
                     /locus_tag="VFR00_04785"
     CDS             1050..2015
                     /locus_tag="VFR00_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HEU0158602.1"
                     /translation="MMQASLEQLLGADGALPLAPDLCQSLEAWLAYLTDARQSSPHTL
                     SAYGRDLRQFLAWLHEGRAQPIALTDLASLDAQRLRAFMAARRRAGLSSRSLARTISA
                     LRAYFRWLEAEEIAENRALLQVALPKVPHGIPKPLSIAKASAVMDGAAAGHGGWLAAR
                     DAAVLLLLYCCGLRISEALGLRRREAPLPGRDVLRITGKGGKERMVPVLPIAQEATRR
                     YLDLCPYPLPADAPLFVGAKGGPLRARIMQLLMQNLRAGLGLPATASPHALRHSFATH
                     LLSAGADLRQIQELLGHASLSTTQVYTEVDRDRLLAVYAKAHPRA"
     gene            2106..2801
                     /locus_tag="VFR00_04790"
     CDS             2106..2801
                     /locus_tag="VFR00_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007821197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU0158603.1"
                     /translation="MAKIALIVGAGSGISAAFARALHADGFSVILAARNTDKLAPLAD
                     EIAGHRVAVDASDVGSVSGLFSDLEHRFQRLDVVLYNASYRTRGAIVDLDPAEVKRAI
                     EVSAFGGFLVAQAAAKRMLKQGEGAIFFTGASASVKGYAQSAPFAMGKFALRGLAQAL
                     ARELQPKNIHVAHFVIDGGVLNAERGRPGDAARPDTLLDPAAIAQSYMAVLKQPRSAW
                     SLEVELRPWVERF"
     gene            2894..3379
                     /locus_tag="VFR00_04795"
     CDS             2894..3379
                     /locus_tag="VFR00_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011662043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEU0158604.1"
                     /translation="MARLIELTLNGRRRIDAVSDNLLLLDYVREQAGLTGTKTGCDGG
                     ECGACTVLVDDYPRLSCLTLAATVAGRRVDTIEALGTAGALSAVQRGFHEKLGSQCGY
                     CTPGFIMACAGLLRRNANPSPEEMRAALGSNICRCTGYVKIIEAVEFAARLLAQGDTA
                     P"
     gene            3376..5721
                     /gene="hcrA"
                     /locus_tag="VFR00_04800"
     CDS             3376..5721
                     /gene="hcrA"
                     /locus_tag="VFR00_04800"
                     /EC_number="1.1.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011156238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoyl-CoA reductase subunit alpha"
                     /protein_id="HEU0158605.1"
                     /translation="MNENVRLQGIGGHVPLVDGPEKVSGRAKYTADLIEPDALVGRIY
                     RSPYAHADILEVDTSQAERLSGVKAVLTGADCAQTFGVLPIARTEHPLARDKVRYKGE
                     PVAAVAAVDAATAERAIRLIRMRVRELPAYFTARAALASDAVALHAKKPGNIERDVNF
                     ELGSIAAGFAEADLVRAATYNCAEVCQNQMEMHAALARYDRVRDRLTVYASTQVPYYV
                     HLMLAQILEMEMSRIRVVKPFVGGGFGCRTECLNIELITALLARRVGGAVRMVISREE
                     TFITHRGRPETDINLKIGLRRDGRITAVDCECVQRGGAHSGYGVVTILYAGSMLYALY
                     DLNNVRYVGRRVLTNTPPCGAFRGHGTVDVRFAFESLLDQMAAELGLDPMAVRRANYL
                     QAPTFTANDLMVNSYGLPACVDWVERESGWATRKGRLPKGKGLGFACSHYISGASKPV
                     NWTGEPHATVKLKLDFDGSIVVLTGAADIGQGSSTVLVQTVAEVLGLDICRVRIVSGD
                     SDVVPKDNGSYSSRVTFIVGNAAIDAANKLKALLLAAAARRLEAEPDDVECLGELYRA
                     GAQDKGLSFEEVVQEALKDSGTITVTGTYSTIPESHGGKKYRGAAIGGTMGYSYSAQV
                     VEVSVDAETGQVSVDQVWVAHDCGKALNALSVEGQVQGSVWMGMGQAMSEEARYHDGL
                     LLTANMLDYRVPTIRDSPPIAVGIVESNDPHGPFGAKEAGEGSLAAFLPALTNAIAAA
                     TGVRFNDLPVTPDRVFAAITRRARQAGEDGRESPTPRAGGA"
     gene            5721..6740
                     /locus_tag="VFR00_04805"
     CDS             5721..6740
                     /locus_tag="VFR00_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011662041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HEU0158606.1"
                     /translation="MEALPQFSLITPGTLADLSGARRAHPQAQLLGGGTDLLVNVRRG
                     IVAPSVLIDVNGVAEIRRLSADADALELGAAVRLEEIERHPAIVQHYPVLAEAAASVA
                     GPTQRNMGTLGGNLCLDTRCIFYNQSEWWRAANHHCLKTTGEICHVAPKSRGVCFATF
                     SGDLAPALAVLDAEVDLLASTGAVRTLPLHELYIGFARHDDPAAGDGKRYLALEAGEL
                     VMRVRARKVPGLRSGYDKLRVRRSIEYPLAGVAVALRRERDSLTDLRVAITGTNPRPV
                     LLEGTARLCGGALDETVYRGLEQLVRDQVMSMKTTFTPGHYRRRIAGVLARRLLDRLF
                     KADAA"
     gene            complement(6757..>7395)
                     /locus_tag="VFR00_04810"
     CDS             complement(6757..>7395)
                     /locus_tag="VFR00_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019964940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HEU0158607.1"
                     /translation="LDIRKPRFGDPLPRTLQHHCGCVIFGGPMSANDGDDFVRSEIDW
                     ITVPLREGKPFLGICLGAQMLTVNLGAKVGFHPRELVEIGYYPLVTTAAAEPLGPFPE
                     HVYEWHKEGFELPRGARLLATSAGAFPNQAFSYGPAAVAVQFHPEITFAQVHRWTGHN
                     PQRLGMKGARSRQEHIEGHVRHGPPVQAWLDRFLRRWLLGGVVQPAASLSGP"
BASE COUNT         1176 a         2521 c         2442 g         1256 t
ORIGIN      
        1 atgggggtgc catgcgtggc cgcgacgcgc accacctctc gcacctcctg tgtggtctcc
       61 acccagatga ccgcgtcggg tggttggttt ggaatccagg tcagcgtgtt ggcatgctgg
      121 cggcgaaggg cctcgcccgt ctgtagacgc tcgccaaagc gctgccgcag gacagcgatg
      181 gccgtagcac tccggtccgc gttcgccgcg ttgccggcac gttctgccat tgccatattt
      241 taccgctcct tgacctggcg cggcagctcc gagcccgccg gcagggccga gccctcagcg
      301 accgtcgccc cggctgccaa aacgatgttc acacagtcgt gtagcaatgt gaagggtccc
      361 ggcattgccc tcgcgcgtat ctctttcgat ctcacctgga gcaaatgccg ccgctgagaa
      421 caagggccgc aggagactac aacgatgctc aacaaaattc taagcttcgc ccgttcagcg
      481 gcccacaacc agaactcccc cgtgttcggt gcgagccggg gcgacacggt gtcggccgaa
      541 aatgtccgcg cgctcgcgac cggcgcggcg ctggcggtgg cgcttgacgc ctccccggcg
      601 gtcctcgtca gcatctacga ttacgcccat cgctttcgcg accatggggc tcggcgcctt
      661 gcctgttgac gccgccgccg ccgggctgcg gccgaacgcg tgcggcccgc ggcggtgggc
      721 tcctgcgtcg ccagccgcgt cggccgcccc tctccacctc gacctctgag cccctgccga
      781 tcgacggcac cgggtccgtc gacattcgtg gcgggcgtgt gccgcgcgcg gcgccgtgcg
      841 ggtccggttg tcagcgccga cccaacggag cggcagcggc gcccaacccc agttctgccg
      901 ctttttccca tttcaggcgc gatgcgactt tgcctggcaa gcgggccttc ggtgctgctt
      961 gtgggctgcg caacccaggt atgtccgggt gcttaacagg gcgttaaccc tcgctgtgga
     1021 tgcttcggtc ggtgcgaaag ccgggccgga tgatgcaagc ttcgctggaa cagctgcttg
     1081 gagcggatgg cgccttgccg ctggcgcccg acctttgcca gagcctagag gcgtggttgg
     1141 cctatctgac cgatgcgcgg caaagctccc cgcacaccct gtcggcctac ggccgcgacc
     1201 tgcgccaatt cttggcctgg ctgcacgagg gtcgagcgca gccaatcgcg ctgaccgacc
     1261 tggcgagcct tgatgcgcaa cggctgcgtg ctttcatggc ggcgcgccgg cgcgccggcc
     1321 tgtcaagccg ctcgctcgcg cgcacgatct cggccttgcg tgcctatttc cgctggctcg
     1381 aggccgagga gatcgccgag aaccgggcct tgctccaggt ggcgctaccc aaagtgccgc
     1441 acggcattcc aaaacccttg agcatcgcca aggccagtgc cgtcatggac ggcgcggcgg
     1501 ccggccatgg cggctggctg gcggcgcgcg atgccgcggt gctgttattg ctctactgtt
     1561 gcggcctgcg catctccgag gcgctcgggc tgcgccgccg cgaggcgcca ctgcccggcc
     1621 gcgacgttct gcgcattacg ggcaagggcg gcaaggagcg catggtcccg gtgctcccca
     1681 tcgcgcaaga ggccacgcgc cgctacctcg acctgtgccc ttatcccctg cctgcggacg
     1741 ctcccctgtt cgtcggggcg aaaggcggcc cgctcagagc ccgcatcatg caactgctga
     1801 tgcagaattt acgcgccggc ctcggtttgc cagccaccgc gtccccgcac gccctgcgcc
     1861 actcctttgc gacgcatctg ctctcggcgg gcgccgacct gcggcagatt caggagctgc
     1921 tcgggcatgc gagcctttcg accacccagg tctataccga ggtcgaccgc gatcgcctgc
     1981 tggccgtcta tgccaaggcg catccgcgcg cctagacatc gggcggtgtc ttgcgccgac
     2041 ggctcgagcg cgggcggcgg atcggctagc atgcgggcac cgccaaccct gctcgaagga
     2101 ccaagatggc caagatcgct ctcatcgtcg gcgccggctc cggcatcagt gccgcattcg
     2161 cccgcgccct tcacgccgat ggcttttccg tgatccttgc cgcgcgcaac accgataaac
     2221 tcgcgccgct ggcggacgag atcgccggcc accgggtcgc agtcgatgcc agcgatgtcg
     2281 gttccgtctc aggcctgttc agcgatctcg agcatcgttt ccagcgcctc gatgtcgtac
     2341 tctataacgc cagctaccgc acgcgcggcg ccatcgtgga tctcgacccc gccgaggtga
     2401 agcgggccat cgaggtctcc gcctttggcg gcttcctggt ggcgcaagcg gcggccaagc
     2461 gcatgctcaa gcagggtgag ggggcgattt tcttcaccgg ggcctcggcc agcgtcaagg
     2521 gctacgccca atccgcgccc ttcgccatgg gcaagttcgc gctgcgcggg ctggcccagg
     2581 cgctggcccg cgagctgcag ccgaagaaca ttcacgtggc tcacttcgtc atcgatggcg
     2641 gcgtgctgaa cgccgaacgg ggccgccccg gcgatgcggc tcggcccgat accctgctcg
     2701 accccgccgc catcgcgcag agctacatgg cggtgctcaa gcagccgcgc agcgcctgga
     2761 gcctggaggt cgagctgcgc ccctgggtgg agcgctttta ggcggcaatt ggtttggtac
     2821 ccaatcgatt tggtgttaag ctcggccgtt cagcgctggc tcggccgggg gccggcgcgc
     2881 gccaggagga gtcatggcac ggctgatcga gcttaccctc aacgggcgcc ggcgcatcga
     2941 tgcggtctcg gacaatctgc tgctgctcga ttatgtccgc gagcaggccg gcctcaccgg
     3001 taccaagacc ggttgcgacg ggggcgaatg cggggcgtgc accgtgctcg tcgacgacta
     3061 cccccgcctg tcctgcctaa ccctggcggc gaccgttgcc gggcggcgcg ttgacaccat
     3121 cgaggcgctc gggaccgccg gcgccttgtc cgccgtgcag cgcggcttcc acgaaaagct
     3181 cggctcgcag tgtggctatt gcacgccggg cttcatcatg gcctgcgccg gtcttttgcg
     3241 ccgtaatgca aaccccagcc cggaggagat gcgcgctgcc ctcggcagca acatttgccg
     3301 ctgcacgggc tatgtgaaga tcatcgaggc cgtcgagttc gccgcgcgcc tccttgcgca
     3361 aggtgacacg gcgccatgaa cgagaacgtg cgcctgcagg gcattggcgg ccacgtaccc
     3421 ctggtggatg ggcccgagaa ggtttccggt cgcgccaaat acaccgccga cctcatcgag
     3481 cccgatgcgc tcgtcgggcg gatctatcgc agcccgtatg cgcacgccga catcctcgag
     3541 gtcgacacct cgcaggccga acgactgtcc ggcgtgaagg ccgtgctgac cggggccgac
     3601 tgtgcgcaga ccttcggcgt gttgccgatc gcgcgcaccg agcaccccct ggcgagggac
     3661 aaagtgcgct acaaagggga gcccgtggcg gcggtggccg ctgtcgatgc ggccaccgca
     3721 gagcgggcca ttcgcctgat ccgcatgcgc gtgcgcgagc tgcccgccta tttcacggcg
     3781 cgggcggcgt tggccagcga tgccgtcgcc ctgcacgcca aaaagccggg caatatcgag
     3841 cgggacgtca acttcgagct cggcagcatc gccgccggct ttgccgaggc cgacctggta
     3901 cgcgcggcga cctacaactg cgccgaggtc tgtcagaacc aaatggaaat gcacgcggcg
     3961 ctggcccgct acgatcgcgt gcgtgaccgc ctgacggtct atgccagcac gcaggtgccc
     4021 tactacgtgc acctgatgct ggcgcagatc ctggaaatgg agatgtcgcg catccgcgtc
     4081 gtcaaaccat ttgtcggcgg cggcttcggc tgtcgcacgg agtgtctcaa catcgagctg
     4141 attaccgcgc tgttggcacg cagggttggg ggcgcggtac gcatggtcat cagtcgcgag
     4201 gaaactttta ttacccatcg cgggcgtccc gagaccgaca tcaacctcaa aatcgggctg
     4261 cgccgggatg gccgtatcac cgccgtcgat tgcgaatgcg tgcagcgcgg cggcgcccac
     4321 tcgggctatg gcgtcgttac catcctttac gccggctcga tgctgtatgc cctttatgat
     4381 ctcaacaacg tgcgctacgt cggcaggcgc gtcctgacca acacgccccc atgcggcgcc
     4441 ttccgcggcc acggcaccgt agacgtgcgc ttcgccttcg agagcctgct cgatcaaatg
     4501 gcggccgaac tgggccttga tcccatggca gtgcgccgcg ccaactatct tcaggcaccg
     4561 accttcaccg ccaatgatct gatggtgaac agctatgggc tgcccgcctg tgtcgattgg
     4621 gtcgagcggg agagcggctg ggcgacccgc aaggggcgtt tgccaaaggg taagggcctt
     4681 ggctttgcct gctcgcacta catttccggc gcctccaagc ccgtgaactg gaccggcgag
     4741 ccacacgcca cggtgaagct gaaattggat ttcgatggct cgatcgtggt gctgacaggg
     4801 gccgccgaca tcggccaggg atcctcgacc gtgctcgtgc aaaccgtggc ggaggtgctg
     4861 ggcctcgaca tctgccgggt gcgcattgtc agcggcgaca gcgacgtggt gcccaaagac
     4921 aatggatctt attcatcgcg cgtgaccttc atcgtcggca acgccgcgat cgatgctgcc
     4981 aacaagctga aggcgttgtt gcttgccgcg gcggcccggc gtctggaagc cgagcccgac
     5041 gacgtcgaat gcctgggcga gctttatcgt gcgggcgcgc aggacaaagg gctctccttc
     5101 gaggaggtgg tgcaggaggc cttgaaggac agcggtacga tcaccgtcac cggcacctat
     5161 tccacgatcc ccgaatcgca cggcggcaag aaatatcgcg gcgccgcgat cggcggcacc
     5221 atgggctaca gctactccgc ccaggtggtg gaagtgagcg tcgatgcgga aaccggccag
     5281 gtcagcgtcg atcaggtgtg ggtcgcgcac gactgcggca aggcgctgaa cgccttgagc
     5341 gtcgaaggcc aggtgcaggg ctcggtttgg atgggcatgg gtcaggcaat gagcgaggag
     5401 gcgcgctatc acgacggcct gctgctgacc gccaacatgc tcgactaccg cgtgccgacc
     5461 atccgggatt cgccgccgat cgccgtgggc atcgtcgaga gcaatgaccc gcacgggccg
     5521 ttcggcgcca aggaagccgg cgaaggctcg ctcgccgcat tcctgccggc cttgaccaat
     5581 gccattgccg cggcgacggg cgtgcgcttc aacgatctgc ccgtcacgcc cgatcgcgtg
     5641 tttgcggcaa taacgcgacg cgcgcggcaa gcgggcgagg acggaagaga aagccccaca
     5701 cctcgagccg gaggcgctta atggaggcgc tgccgcagtt ttccctcatc acgccgggca
     5761 cgctggcgga tctttccggc gcgcggcggg cccatcctca ggcgcaactg ttgggcggcg
     5821 gcaccgatct gctggtcaac gttcggcgcg gcatcgtcgc gcccagcgtc ctgatcgacg
     5881 tcaatggcgt ggctgaaatc cgcaggctca gcgccgatgc cgacgccctt gagcttggcg
     5941 ctgcggtgag gctggaagaa atcgagcgcc accccgccat cgtccagcac tatcccgtgc
     6001 tggccgaggc cgccgcctcg gtcgcgggac caacgcaacg caacatgggc acgctcggcg
     6061 gcaacctttg cctcgatacc cgttgcatat tctacaacca aagcgaatgg tggcgcgccg
     6121 ccaaccacca ctgcctcaag accacgggcg agatatgcca cgtggcgccc aagagccgcg
     6181 gcgtctgctt tgctacgttc agcggcgact tggcgcccgc gctggcggtg ctcgatgccg
     6241 aagtcgatct cctcgccagc actggcgcgg tgcgaacctt gccgctgcac gagctctata
     6301 tcggctttgc gcgccacgac gatcctgcgg ccggcgacgg caagcgctac ctggcgctcg
     6361 aggccggcga gctggtcatg cgcgtgcgcg cgcgcaaagt cccgggcctg cgctcgggct
     6421 acgacaagct tcgggtgcgc cgctcgatcg aatatccgct cgccggtgtc gccgtcgcct
     6481 tgcgacgcga gcgcgatagc ctcaccgact tgcgcgtcgc catcaccgga accaatccac
     6541 gcccggtgct gctggaaggc accgcacggc tatgcggcgg ggcactcgat gagacggtct
     6601 accgcggtct ggaacagctc gtgcgcgacc aggtgatgtc catgaagacg accttcaccc
     6661 cggggcacta tcggcgccgc atcgccggcg tcctggcacg gcggctcctc gacaggcttt
     6721 tcaaggccga cgccgcctga gttgacttgg gtgcggtcac ggacccgaca agctggctgc
     6781 cggttgcacc actccaccca acagccagcg gcgcaaaaag cggtcgagcc aggcctgtac
     6841 cggggggccg tgcctgacgt ggccctcgat atgctcttgg cgcgagcgcg cacccttcat
     6901 gccgaggcgt tgcggattgt ggccggtcca gcggtgcacc tgcgcgaagg tgatctcggg
     6961 atgaaattgc acggcaacgg ctgccggacc atagctgaag gcctgattcg gaaaggcgcc
     7021 cgctgaggtg gcaagcagcc gggcaccgcg ggggagctcg aagccttcct tgtgccattc
     7081 gtaaacatgc tcggggaatg ggccgagcgg ctccgccgcc gcggtcgtga cgagcgggta
     7141 atagccgatc tcgaccagct cccgggggtg gaagcccact ttggcgccga gattgacggt
     7201 cagcatctgg gccccgaggc agatgcctag gaagggcttg ccctcccgca acggcaccgt
     7261 gatccagtcg atttcgctgc gcacgaaatc atccccgtca ttggcgctca tcggcccgcc
     7321 gaagatcacg cagccgcagt gatgctgcag cgtccgtggc agcggatcgc cgaagcgcgg
     7381 cttgcggatg tcgag
//
LOCUS       DASTMT010000206         2261 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_108782, whole genome shotgun sequence.
ACCESSION   DASTMT010000206 DASTMT010000000
VERSION     DASTMT010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2261)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2261)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2261
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_108782"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..343
                     /locus_tag="VFR00_04815"
     CDS             <1..343
                     /locus_tag="VFR00_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012333344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEU0158608.1"
                     /translation="ILRRGRVANRPDLLRFGRLEIDRAAREVRLDGEVKQLTSHQFAL
                     LLALAEHAGRVMSRDSLMDIVKMEPLEVFDRSIDVHISRIRAAIEDEAKRPRRILTVR
                     GAGYVFAKQQD"
     gene            352..1605
                     /locus_tag="VFR00_04820"
     CDS             352..1605
                     /locus_tag="VFR00_04820"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU0158609.1"
                     /translation="MRRIYHQFYLTILASLLAVVVMAGALWRFAPTETPSDQAFEMAG
                     VLVATQLAPAGAPLAAQQQAIEQLYARLGLDIGLFDAERRPLAAVGDPVPPPGARQSG
                     GWVYGRYGPAWAIRLPDGRWVVARPPPRQRRPVLGIVAFLAAIALLVAICAYPLVRRL
                     TRRLERLQAGVESLGAGDLAARVKVEGKDEVASLAASFNRAAQRIEELVGAHKMLLAH
                     TSHELRTPLARIRLGIELLQSDPDAKRKANLERDIAELDALIEQILLSSRLGAVSAVD
                     RSEEVDLLAVAAEEAARYADCSVAGEPVVVRGDRGLLQRMVRNLLDNAQQHGVAPIAV
                     NVSAAKDAATLLVCDHGPGIAPEDRERIFSPFYRAAAASGAAGTGLGLTLVRQIARQH
                     GGDASWTLTPSGLNAIRVVIARAPG"
     gene            1699..>2261
                     /locus_tag="VFR00_04825"
     CDS             1699..>2261
                     /locus_tag="VFR00_04825"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU0158610.1"
                     /translation="MGISAAVDITAKRVLVTGAAGFIGFHTCHKLLARGCRVLGLDDL
                     NAYYDVALKHARLARLDGVQGFAFERCDVADKTRVLSLIADFQPHYVVHLAAQAGVRF
                     SLQDPSQYVRANVDGFLAVLEACRAQPVSHLLYASSSSVYGANTKVPFCETDRVDDPR
                     SLYAVTKRTNELMAHTYAELFGIAASGL"
BASE COUNT          338 a          781 c          743 g          399 t
ORIGIN      
        1 cattctgcgg cgcggaaggg ttgccaatag gcccgacctc ctgcgctttg gccgcctgga
       61 gatcgatcgg gccgcgcgcg aagtacgcct cgacggcgag gtgaagcagc tcaccagcca
      121 tcaattcgcg ctgctgttgg cgctcgccga gcacgccggg cgcgtaatgt cgcgcgatag
      181 cctgatggac atcgtgaaaa tggagccgct cgaggtcttc gaccgttcca tcgacgtgca
      241 tatctcgcgc atccgcgcgg ccatcgaaga cgaggcaaag cggccgcgcc gcattctcac
      301 cgtacgcggt gccggctacg tcttcgccaa gcagcaggat tgaacggcgg catgcgtcgc
      361 atctatcatc aattctacct aaccatactc gccagcctgc tcgccgtcgt cgtcatggcc
      421 ggcgcgctgt ggcgctttgc accgaccgaa acgccgagcg accaagcctt cgaaatggcg
      481 ggcgtcctgg ttgcgacgca attggcaccc gccggtgcgc cgctcgcggc acagcagcag
      541 gccatcgagc aattgtatgc ccgtctcggc ctggacatcg gcttgttcga tgcggagcgc
      601 cggcccttgg cggccgtagg cgaccccgtg ccgccgccgg gagcccgcca gagcggcggc
      661 tgggtctatg ggcgctacgg gcccgcctgg gcaattcgcc tgcccgacgg ccgctgggtg
      721 gtcgcccgtc cacccccgcg ccagcgccgt ccggtgttgg gcatcgtggc ctttcttgcc
      781 gcgatcgccc ttttggttgc gatctgcgct tacccgctgg tgcgccgcct gacgcgccgg
      841 ctggagcggc tgcaggcggg cgtcgagtcg ctgggggccg gcgaccttgc ggcgcgcgtc
      901 aaggtcgaag gcaaggatga ggtcgcaagc ttggcggcga gcttcaatcg cgcggcccag
      961 cgcatcgagg agctggtggg agcccataaa atgctgctcg cgcatacctc gcacgaattg
     1021 cgcacaccgc tcgcacgcat ccggctcggc atcgagcttc tgcaatcgga tccggatgcc
     1081 aaacgcaagg caaacctcga gcgggatatc gcggagctcg acgccctcat cgagcaaatt
     1141 ctgctctcca gtcgcctcgg tgccgtatcc gccgtggaca ggagcgagga ggtcgatctg
     1201 ctcgcggtcg cagccgagga agccgcgcgc tatgccgatt gctccgtggc cggcgagccg
     1261 gtcgtggtgc gtggcgaccg cggtctgttg cagcgcatgg tgcgcaacct cctcgacaat
     1321 gcacagcagc acggggttgc cccgatcgcc gtcaacgtca gcgccgccaa ggacgccgcg
     1381 acgctgctgg tctgcgacca cggcccgggg atcgcgcccg aggaccgcga gcgcattttc
     1441 tcccccttct atcgtgccgc cgctgccagt ggcgccgccg gtaccgggct gggcctgacc
     1501 ttggtgcgcc agattgcgcg ccagcacggc ggcgatgcca gctggacctt gacgccatcg
     1561 ggcctcaatg ccattcgcgt cgtgatcgcc cgcgcccccg gttgaggcca gcggccgctc
     1621 cagcaccggg cgcagcgggc ctcgcccggc tcccccgttg cgggcgacgt ggtaaggtgg
     1681 cgccggagca tcgaggacat gggcatctct gcggcggtcg atatcacggc aaagcgcgtc
     1741 ctcgtcaccg gggcggcggg tttcattggc tttcacacct gtcacaagct ccttgcccgc
     1801 ggctgtcgcg tgctcgggct ggacgacctc aatgcctact acgatgtggc cctcaagcac
     1861 gcccgcctgg cgcggctcga tggcgttcag ggttttgcct tcgagcgctg tgacgttgcc
     1921 gacaagacgc gcgtgctaag cctgatcgca gacttccagc cgcactatgt ggtgcacttg
     1981 gcagcacagg ctggcgtgcg tttttccctg caggatccct cccagtacgt gcgcgccaac
     2041 gtcgatggct tcctggcggt gttggaggcc tgccgggcac agccggtcag ccacttgctc
     2101 tatgcctcct cgagctcggt ctatggcgcc aacaccaagg tgccgttctg cgaaaccgat
     2161 cgggtcgacg acccccgctc gctttatgcc gtgaccaagc ggaccaacga gttgatggcg
     2221 catacctacg ccgagctctt cggcattgcc gcctccgggc t
//
LOCUS       DASTMT010000207         6387 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_2078759, whole genome shotgun sequence.
ACCESSION   DASTMT010000207 DASTMT010000000
VERSION     DASTMT010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 6387)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6387)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6387
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2078759"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..927
                     /locus_tag="VFR00_04830"
     CDS             <1..927
                     /locus_tag="VFR00_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012335523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0158611.1"
                     /translation="ASNIWQLTIAHGCIGFGSSATFAPLIADISHWFERRRGVAVSIC
                     ATGSYLAGTLWPPVVNQFVASDGWRQTQVGIGLFCLISMLPLITLLKRRIALVQGLSA
                     GGNGATAATSLGLSSGVLQALILVAGVACCVAMSMPQVHIVAYCGDLGYGVARGAEML
                     SVMLGFGIISRVGSGFIADRIGGLATMLLGSALQAGALLLYLWFDGLTSLYVISAFFG
                     LVQGGIIPMYAVIVRDFFPPHEAGARVGLAIMATIVGMALGGWLSGAIFDLTGSYAQA
                     FSNGIAWNLINVSIVLFLMLRQAGRRHLMAPA"
     gene            complement(1055..1372)
                     /locus_tag="VFR00_04835"
     CDS             complement(1055..1372)
                     /locus_tag="VFR00_04835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0158612.1"
                     /translation="MTDHPRFEPLRGSPDTMHLRYHGGALVASVLRLADQWVAQPAWP
                     QRPRVPGKPARDVRRARLRVLLTGLLMLGAVLLTALWAPGRQAGNRPAPPQAPQKVAV
                     APA"
     gene            complement(1506..2771)
                     /locus_tag="VFR00_04840"
     CDS             complement(1506..2771)
                     /locus_tag="VFR00_04840"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0158613.1"
                     /translation="MPVFDPAPALPPPAKAARQRVFYGWYVVAAIALITTTSSGFAFY
                     NLSILLAAFVSERGFPVALASSATATFFLASGIGGAIAGRLVDRIDARLVISANASIG
                     ALVLASVGLLTEVWQLFLFHVVFGLAHGSSGLVPVTTVLARWFNVRRALAFSLASTGL
                     SLGGIVIAPIVAFAVQRQGLAGAAPYMGAALFLGIVPVTLSVLRPSPQAMGLAPDGMA
                     HSGADRGERGGQGSTSFAEAIRTRYFYAVSIAYLCLLGAQVGAIAHLFRLVSGRAGLE
                     TAAFAIASMAAASTIGRLIGGLITLRLPVRTFALGLMAQQALGLACLALSFEPVAILA
                     SVVLFGLTIGNSLMMHPLLLAETFGTRDYGRIYSTSQLLTITGVAGCPAVIGVLYELS
                     GGYAAPFLGAAALSVLGLAALGLNRIERR"
     gene            3104..3679
                     /locus_tag="VFR00_04845"
     CDS             3104..3679
                     /locus_tag="VFR00_04845"
                     /inference="COORDINATES: protein motif:HMM:NF016874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HEU0158614.1"
                     /translation="MTKTPQRRRIYLAGPEVFLPNALELAAEKCRLAAEAGLEGVFPH
                     DPKLKAQLAGNGSAAECIFSANTALMSSCDALIANLTPFRGVSADAGTVFELGFMFAL
                     RRPIAAYTNTAQSYHARTTVFRGRAGFADADRPECEVEDHDLSDNLMLEMAVIASGAR
                     LIRHPSPGTPDMTDLTAFRRCLAALARRLGS"
     gene            complement(3674..4876)
                     /locus_tag="VFR00_04850"
     CDS             complement(3674..4876)
                     /locus_tag="VFR00_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012045334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low temperature requirement protein A"
                     /protein_id="HEU0158615.1"
                     /translation="MLIENSQQSLLRGKPENGHHRVTFVELFFDLVFVFAVTQLSHSL
                     LEHLSLLGALQTTLLLMAVWWVWIYTSWVTNWLDPDATPVRLMLFVLMVAGLLLSTSL
                     PQAFGARGWAFAGAYVAMQLGRSLFTLWALAGRNEVNFRNFQRISAWLGLSALFWFAG
                     ACVAEQARLPLWALALLIEYASPALGFWTPGLGRSTTADWDVEGGHLAERCGLFVIIA
                     LGESIVVTGAKFATLAWTTATVTAFVAALIGTIAMWWIYFNIGAERASARIMASADPG
                     RLARLAYTYVHIVLVAGIIVCAVADELILSHPWDETETATRAVLIGGPALYLIGNILF
                     KRATASRMVLSHFVGLGLLGGLSLAALPPLPLGAASSLVMVLVATWETLSLKGEGVSP
                     AGAPTRPS"
     gene            complement(4904..5617)
                     /locus_tag="VFR00_04855"
     CDS             complement(4904..5617)
                     /locus_tag="VFR00_04855"
                     /inference="COORDINATES: protein motif:HMM:NF016300.4"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HEU0158616.1"
                     /translation="MLRHNMAVAFGGLALATTLLLATAGLSAEGDAQQERTVSVSATG
                     SVVAEPDLAMISTGVVSEADTAREALSRNTATMAKVIDGLKALGISAQDIRTTSLNVE
                     PRYRSAKDQAPSIVGYQVVNQVRITARELKRLGEVLDQIVGLGVNQIGGIAFEVSKAE
                     ELRDEARKMAMANALRRARLYAASAGADVGAVLMISEDAPAPAPRPMARTSLAASDVP
                     IEPGTQRLEVKVYVTWALR"
     gene            5739..5813
                     /locus_tag="VFR00_04860"
     tRNA            5739..5813
                     /locus_tag="VFR00_04860"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5772..5774,aa:Ile,seq:cat)
     gene            complement(6259..>6387)
                     /locus_tag="VFR00_04865"
     CDS             complement(6259..>6387)
                     /locus_tag="VFR00_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020179203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HEU0158617.1"
                     /translation="DPDCDLDYVPKVGRDKRVTYAMSNSFAFGGLNAVLVFGPAPN"
BASE COUNT         1142 a         2137 c         2127 g          981 t
ORIGIN      
        1 gccagcaata tctggcaact gacgatcgcg cacggctgca tcggcttcgg cagctcagcc
       61 acctttgcgc cgctgatcgc cgatatctcg cactggttcg agcggcggcg cggtgtcgcg
      121 gtctccatct gcgcaaccgg cagctatctc gccggcacgc tgtggccgcc ggtcgtcaac
      181 caattcgtcg ccagcgatgg ctggcgccag acgcaggtcg gcattggcct gttctgcctc
      241 attagcatgc tacccctgat cacgctcttg aagcggcgca ttgccctggt gcaggggctc
      301 tcggccggcg gcaatggcgc aaccgcggcg acatccctcg gtctctcgag cggcgtcctg
      361 caggcgttga tcctcgtcgc aggtgtcgcc tgctgcgtgg ccatgtcgat gccgcaggta
      421 cacatcgtcg cctattgcgg ggatctcggc tatggcgtgg cgcgcggcgc cgagatgctc
      481 tccgtcatgc tgggcttcgg cattatcagc cgcgtcggct ccggcttcat tgccgatcgc
      541 atcggcgggc tcgccaccat gctgttgggt tccgcgctcc aggccggggc gcttctgctc
      601 tacctttggt ttgacgggct cacctcgctt tacgtgatct cggccttttt cgggctggtg
      661 cagggcggca tcattccgat gtacgccgtg attgtgcgcg atttctttcc gccgcacgag
      721 gcaggggctc gtgtcggcct cgccatcatg gcgaccatcg tcggcatggc gctcggcggc
      781 tggttgtcgg gtgccatctt cgacctgacg ggctcttatg cccaggcctt cagcaacggc
      841 atcgcctgga acctcatcaa tgtctcgatc gtgctgttct tgatgctgcg gcaggccgga
      901 cgccgccacc tcatggcacc tgcctaagag gtccgtgctg gctgtgccgg tcacggcggc
      961 acgagcatca gatcagccaa acttgcggcc gaacggcgcc cgcaaaatgg cgagcacgcc
     1021 tgccgtgccg ctggagaacc gcaggcacgc cgcctcaggc gggggccacg gcgaccttct
     1081 gcggcgcctg gggcggtgct ggacgattgc ccgcctgccg gccgggcgcc cacagggccg
     1141 tcagcaggac ggcccccagc atgagcaatc cggtcagcaa cacccgcaag cgagcacggc
     1201 gcacgtcgcg cgccggcttg ccgggcacac gcgggcgctg cggccacgcc ggctgcgcga
     1261 cccattggtc ggcaagccgg agaacggagg caacgagagc acctccatga tagcgaaggt
     1321 gcattgtgtc gggggaaccc ctgagcggct cgaatcgcgg atgatcggtc atgacacaat
     1381 cgcctctgcg cctgcgcctg ccggcgcccc accccccagc ctaatgcgcg ggcgatttgc
     1441 cgctgtgccc tggagcacag gcgagcggcg gtccgccaag cggtgcccat tgagccacgc
     1501 gcctgtcagc gacgctcgat gcgattgagc ccgagggcgg ccaacccgag cacgctcaag
     1561 gccgcggcac ccagaaacgg cgcggcgtac cccccgctca gttcatagag gacgccgatc
     1621 acggctggac agcctgcgac cccggtaatg gtcaggagct ggctcgtcga gtagatgcgg
     1681 ccgtaatcgc gtgtgccgaa cgtctcggcc agcaacaggg gatgcatcat gagcgagttg
     1741 ccgatagtga gaccgaacag caccacgctg gcgaggatcg ccacgggctc gaaactgagc
     1801 gccaggcagg cgaggccgag ggcctgctgt gccatcaggc ccagggcgaa cgtccgcacg
     1861 ggcagccgca acgtgatgag gccgccgatg aggcggccga tcgtactggc cgcggccatg
     1921 ctggcgatgg caaaggcggc ggtttcgagg ccggcccggc cgctgaccaa ccgaaagagg
     1981 tgggcaatgg cgcccacctg cgcccccagc agacacaggt aggcaatgga tacggcatag
     2041 aaataacgcg tacgaatggc ttcggcgaac gacgtcgagc cttgaccgcc gcgctcgcct
     2101 cgatcggcgc ctgagtgcgc catgccatcg ggcgccaacc ccatggcctg tggcgagggc
     2161 cgcagcaccg agagcgtaac cggcacgatc cccaaaaaca gcgccgcccc catatagggc
     2221 gccgccccgg cgagcccctg ccgctgcacg gcgaaggcca cgatcggggc tatgacgatg
     2281 ccgcccagcg acaggcccgt tgaggcgagg gaaaaggcca gcgcacggcg tacattgaac
     2341 cagcgcgcca gcaccgtcgt aaccggcacc aaaccgctcg acccgtgcgc caacccgaat
     2401 acgacgtgga acagaaagag ctgccatacc tcggtgagca ggcccaccga agccaacacc
     2461 agcgcgccga tgctggcgtt ggcggaaatg acaagccgcg cgtcgatgcg atcgacgagg
     2521 cgcccggcga tggcgccacc gatgccgctc gcgaggaaaa aggtggcggt ggcgctcgac
     2581 gccaacgcca ccggaaagcc gcgctcgctg acgaacgccg ccagcaggat cgataggttg
     2641 tagaaggcaa agcccgacga tgtcgtggtg atgagggcga tcgccgccac cacgtaccag
     2701 ccgtaaaaca cgcgctgccg cgccgccttc gctgggggcg gcagcgccgg ggcgggatcg
     2761 aagaccggca aggacacctc tgcgggagcg aaagtagggc gagggcgggc cctggacgac
     2821 gcggccgcgt ttggcgccgc cacccccgac gccgaccagg ggagggcagc aatggccgag
     2881 gcagccgcgc tcggcaaggg caaatctcca cgccgcggcg ggcgccacgc ccggccgcct
     2941 ggcaaaggcc tgtgggcatc cggtccttgc gacggttcgc tccgctgcca tcaatgctca
     3001 cgctgtgcta aggcgcccca tcggccgaag gccccgcagc agcggcaggc gccggacgtg
     3061 cgccttacga cattcgcgcc aacgagaagg ctcattcggc agcatgacga agaccccgca
     3121 gcgccgacgt atttatcttg ccggccccga ggtcttcctg cccaacgcgt tggagcttgc
     3181 cgccgagaag tgccggctgg ccgcggaggc cggtctcgag ggcgtctttc cgcatgatcc
     3241 caagctcaag gcgcagctgg caggcaacgg cagcgcggcc gagtgcatct tttccgccaa
     3301 cacggcgcta atgtcttcct gcgacgcctt gatcgccaat ctgacgccgt tccgcggtgt
     3361 atccgccgat gccggcacgg tgttcgagct gggcttcatg tttgccttgc gccggccgat
     3421 cgccgcctac accaatacgg cgcagagcta ccatgcccgc accacggtgt tccggggccg
     3481 tgcggggttt gcggatgccg accggccgga gtgcgaggtc gaggatcatg acctcagcga
     3541 caatctgatg ctcgagatgg ccgtgatcgc gtcgggtgcg cggttgatcc gacaccccag
     3601 ccccggcacc ccggatatga cggatctcac cgcctttcga cgctgtcttg cggcgctggc
     3661 ccggcgcttg ggctcatgaa gggcgcgtcg gggcgcccgc gggggatacg ccctcgccct
     3721 tgagtgacag ggtttcccag gtcgccacca gcaccatgac caggcttgag gccgcgccca
     3781 agggcagggg cggcagggcc gccagactga ggccgccgag gagcccaagg cccacgaaat
     3841 gcgagagcac catgcggctg gccgttgccc gtttgaacag aatgttgccg atcagataga
     3901 gcgccggccc accaatcagg accgccctgg tcgcggtctc cgtttcatcc caggggtgcg
     3961 aaaggatcag ttcgtcggca accgcgcaga cgatgatgcc ggcgaccagg acaatgtgca
     4021 cataggtata ggctagcctt gccaagcggc cgggatcggc cgaggccatg atgcgggcgc
     4081 tggcgcgctc ggcaccgata ttgaagtaga tccaccacat ggcaatggtg ccgatcaagg
     4141 cggccacgaa cgccgtcacg gtcgcggtgg tccatgccaa tgtggcaaat ttcgcaccgg
     4201 taacgacgat cgattcgccc agcgcaatga tgacgaacaa gccgcagcgc tcggccaggt
     4261 gaccgccttc cacgtcccag tccgccgtgg tcgaccgccc cagacccggc gtccagaagc
     4321 cgagcgccgg ggaggcgtat tcgatgagga gtgcaagggc ccacagcggc agccgggcct
     4381 gctcggcgac acacgctccg gcgaaccaga acagcgccga gaggccgagc caggcactga
     4441 tccgttgaaa gttgcgaaaa ttcacctcgt tgcggccggc aagcgcccag agcgtgaaaa
     4501 gcgagcggcc gagttgcatt gccacgtagg ccccggcaaa ggcccagccg cgggcgccga
     4561 acgcctgcgg cagcgaggtc gacaacagga gacccgcgac catcagcacg aacagcatca
     4621 gccgcaccgg ggtcgcgtcc ggatcgagcc agttggtgac ccaggacgtg tagatccata
     4681 cccaccacac cgccatcagc agcagcgtcg tttgcagggc gccgagcagc gagaggtgtt
     4741 cgagcaggga atgcgagagc tgcgtcacgg caaagacgaa gacaagatcg aagaacagct
     4801 cgacgaaggt gacgcggtgg tgcccattct cgggcttgcc acgtaacagg ctttgctggc
     4861 tgttttcgat caacatgccc ggacccccgc gagacccagg ccgctagcgc agagcccagg
     4921 tgacgtagac tttcacctcg agacgctggg tgccgggctc gatcggtacg tcgctggccg
     4981 ccagcgaggt gcgcgccatc gggcggggcg ctggggcggg agcatcttcc gagatcatca
     5041 ggaccgcgcc aacgtccgcc cccgcactgg cggcatagag ccgggcgcgg cgcagggcat
     5101 tggccattgc catcttgcgt gcctcatccc gcaactcctc cgccttcgag acctcgaagg
     5161 cgataccacc gatctggttg acgccgaggc cgacgatctg atcgagcacc tcgcccaggc
     5221 gcttgagctc gcgcgcggtg atgcgcacct gattgacgac ctgatagcca acgatgctgg
     5281 gggcctgatc cttggcgctg cgatagcgcg gctcgacatt gaggctggtt gtgcggatgt
     5341 cctgcgcgga aatgccgagg gccttcaacc cgtcaatgac cttggccatc gttgccgtat
     5401 tgcgcgatag ggcctcccgg gccgtatcgg cctcggagac gacgccggtg gagatcatcg
     5461 ccagatcggg ctcggcaacc acgctgccgg tggccgaaac gctgacggtg cgttcttgct
     5521 gcgcgtcccc ttcggccgac agaccggccg tggcgagcaa aagtgtggtc gccaaggcaa
     5581 ggccgccgaa cgccaccgcc atattatgtc tcagcatacc atcctcgcct gggcccgaac
     5641 gtcccggcac gttaaggcgc cattttggct caagcatgcc gcccagcgcc attccaagca
     5701 accatctccg ggtgctatag aggcgctccc ctcgcgtcgg gcccgtagct caatggttag
     5761 agccggcggc tcataaccgt ctggttgcag gttcagtcct gccgggccca ccatctctct
     5821 accgcaggcc gttggggttt cggtggcttg acggtgttca cgagccgctc gtcacgagct
     5881 ttgccatcgc tgctcacgcc aagcgcgttt tgtcctgtgc cctataaagc gccgcttgag
     5941 caaggtcggt agggcgggct tgccgatgaa atggtgcaca gcggggctta ctcgatcgcg
     6001 gaggcgcagt cagccgtcgc cagacggggt gaccgaaatc agcgaccagc atggcgagag
     6061 gcatggggac gatcgcctcg gcggcacggt gagctctacg acgtagcccg cccgcggcgc
     6121 ttcggttcca gcgtggcatg cagctgagtg gaacagcctt cgaagatgcg cgatcccacg
     6181 gcgcgtcgac tgagacgcgg gctaagggga gccgaggagg acgaggccgc caagcccagg
     6241 cgcttgcccg ccaagggcct agttgggggc cggcccgaac accagcacgg cgttgagccc
     6301 accgaatgcg aaggaattcg acatggcata ggtcacgcgc ttgtcgcggc ccactttggg
     6361 cacgtaatcg agatcgcaat cgggatc
//
LOCUS       DASTMT010000208         2196 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_1747979, whole genome shotgun sequence.
ACCESSION   DASTMT010000208 DASTMT010000000
VERSION     DASTMT010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2196)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2196)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2196
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1747979"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..429)
                     /locus_tag="VFR00_04870"
     CDS             complement(<1..429)
                     /locus_tag="VFR00_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006467167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEU0158618.1"
                     /translation="MSSTAQASAWEPRYAHRAQRMVASEIRELLKVLERPNIVSFAGG
                     IPDPKLFPVAEIGAAYAEVLSHPTSAGAGLQYSVSEGYAPLRRWIADDMSRHGVPCAP
                     DNIVITNGSQQALEFLARLLISPGDTALVGMPTYLGALQAF"
     gene            complement(569..1849)
                     /gene="glp"
                     /locus_tag="VFR00_04875"
     CDS             complement(569..1849)
                     /gene="glp"
                     /locus_tag="VFR00_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006935081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HEU0158619.1"
                     /translation="MDKKLLDDCFLSDRPRLRHEQALAILRERVRPVVGVEHVGLAAA
                     LNRILANPVIASQDVPAHTNAAVDGYSFAAADYRADSGATLALGGRAAAGHPLSAAPP
                     PGTAVRIFTGAVMPLGHDTVAMQEDTQPRLEQERAVVGIPAGLKPNANVRKAGEDVEA
                     GETVLGGGSLLRPQDLAALAALGLGEVACYARLKVGILASGDEIVRPGGALKPGQVYD
                     ANTPMLMGLISAAGAEVVDLGVLPDDAETVRQRLAETAAAYDVIITSGGASRGEEDHL
                     ATALDVLGKRHLWQLAIKPGRPMSFGQIGNAVVVGLPGNPVAVFVCFLIYVWPMLRRL
                     GGAPWPEPRRWRLPAHFAFPQRKVGRREFWRGTLVPAPPGWAVVKFPRDGSGLISGLR
                     AADGLIEIPEDVAEVNWGDPVAFLPFSEFGIASR"
     gene            complement(1983..>2196)
                     /locus_tag="VFR00_04880"
     CDS             complement(1983..>2196)
                     /locus_tag="VFR00_04880"
                     /inference="COORDINATES: protein motif:HMM:NF018829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MlrC C-terminal domain-containing protein"
                     /protein_id="HEU0158620.1"
                     /translation="SNLGIEPARKRILLLKSTNHFMAAFGPIAKLVLYADSDAPLARD
                     YRRIPYTRVERPIWPLDEITQPRLIF"
BASE COUNT          403 a          709 c          756 g          328 t
ORIGIN      
        1 aaaggcttgc agggcaccga gataggtcgg catgcccacc agcgccgtat cgccggggga
       61 gatgagcagt cgggccagaa attcgagggc ttgctgcgag ccgttggtga tgacgatatt
      121 gtcgggcgcg caagggacgc cgtggcgaga catgtcatcg gcgatccacc gccgcagcgg
      181 cgcataaccc tcgctcaccg agtattgcag gcctgccccg gcggaggtcg gatgcgacaa
      241 gacctccgcg taggcggcgc cgatctcggc cacaggaaac agcttcggat cggggatgcc
      301 gccggcgaag gagacgatgt tggggcgctc caagactttg agcagctccc ggatctcgga
      361 tgcaaccatg cgttgggcgc ggtgcgcgta tcgtggctcc catgcgctgg cctgagcggt
      421 gctcgacatc gctgcctcct gggctattcc gttgggcccg gagacaatta tgtcagcaat
      481 actgacctat ttgcaaggcg gcctataaag ctttgtcaag catagagaat ttagcgcgtg
      541 gagggagcgg ggctggcgcg gcagcgattc agcggctggc gatgccgaat tcggaaaagg
      601 gcagaaaggc aaccgggtcg ccccagttca cctccgccac gtcttccggg atttcgatca
      661 gcccgtcggc ggcgcgcagg ccggagatca ggcccgaacc atcgcgcgga aacttgacga
      721 ctgcccagcc cgggggcgca ggcaccagcg taccgcgcca gaattcgcgc cgtcccacct
      781 tgcgctgggg gaaggcgaaa tgcgccggca agcgccagcg gcgcggctcc ggccagggcg
      841 cgccgccaag gcgtcgcagc atcggccaca cgtagatgag aaagcagaca aacacggcaa
      901 cggggttgcc gggcagaccg accacgacgg cgttgccaat ttgaccgaag ctcatgggcc
      961 gcccaggctt gatggcaagc tgccagaggt gccgcttgcc cagcacatcg agcgccgtcg
     1021 ccagatggtc ttcctcgccg cggctggcgc ccccggaagt gatgatcacg tcgtaggccg
     1081 cagccgtttc ggccagccgc tgccgtacgg tctcggcgtc gtcaggcaac acgccaaggt
     1141 cgacgacctc ggcacccgcc gcgctgataa gacccatcaa catcggcgtg ttggcatcgt
     1201 agacctgtcc cggtttgagg gcgccgccgg ggcgcacaat ctcatcgcca ctggctagga
     1261 tgccgacctt gagccgggca taacaggcga cctcgccgag ccccaaggcg gcaagcgcgg
     1321 caaggtcctg cggccgcagc aaagagccgc cgccgagcac cgtctccccg gcttcgacgt
     1381 cctcgcccgc cttgcgcaca ttggcgttcg gcttgaggcc agcaggaatg cccacgaccg
     1441 cgcgctcctg ctccaggcgg ggttgggtat cttcctgcat ggccacggta tcgtggccga
     1501 gcggcatgac ggcaccggta aagatgcgca ccgccgtgcc tggcggaggc gcggcgctga
     1561 gcggatgacc ggcggcggcc cgcccgccga gcgccaaagt cgcgccgctg tccgcgcgat
     1621 agtcggcggc ggcaaaggag taaccatcga cggcggcgtt ggtgtgggcg ggcacgtcct
     1681 gcgaagcgat gacggggttg gcgaggatcc ggttgagggc agcggccagg ccaacgtgct
     1741 caacgccgac aaccggccgc acgcgctcgc gcaggatggc cagcgcctgc tcatggcgca
     1801 ggcgcggccg atcgctcaaa aaacagtcgt cgaggagctt cttgtccatg ccgtcgttga
     1861 ggcgccttgc ccgtcgttgc caaactccac ccggccgccg cgcaatctaa ccaaaaacat
     1921 ggcggcgagc tatgcgccgg gcggccggcg ggatgacaag ccggtccggc ggcaccgcct
     1981 gctcaaaaga tcagccgcgg ctgggttatc tcgtcgagcg gccagatcgg acgctcaacg
     2041 cgggtatagg ggatgcggcg atagtcgcgc gcgagcggcg cgtcgctatc agcgtagagc
     2101 accaatttcg caatcggccc gaaggcggcc atgaaatgat tggtggactt gagcagcaaa
     2161 atccgcttgc gcgccggctc gatgccaagg ttgctg
//
LOCUS       DASTMT010000209         4369 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_2404740, whole genome shotgun sequence.
ACCESSION   DASTMT010000209 DASTMT010000000
VERSION     DASTMT010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4369)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4369)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4369
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2404740"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..541
                     /locus_tag="VFR00_04885"
     CDS             <1..541
                     /locus_tag="VFR00_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HEU0158621.1"
                     /translation="MGFCIFSNAAIAALYARKTYGVERVAVVDFDVHHGNGTQDVFWS
                     DKNLLFASTHQMPLYPGTGAISETGVGNIFNAPLRPGDDGERFKEAFEARLSPALKNF
                     APDLVLISAGFDAHKDDPLANLRLLEGDFAWATGKIAELAATHAGGRLVSMLEGGYNL
                     TALARSVGVHVKMLMEAGA"
     gene            576..848
                     /locus_tag="VFR00_04890"
     CDS             576..848
                     /locus_tag="VFR00_04890"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005766408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEU0158622.1"
                     /translation="MQTSKPAAKHLDIAGMSFERALKELESIVGRLERGDVELEESIT
                     IYERGEALKEHCDRLLKQAEARVERLTLSETGTPTGTEPFEPTNSA"
     gene            977..2902
                     /gene="dxs"
                     /locus_tag="VFR00_04895"
     CDS             977..2902
                     /gene="dxs"
                     /locus_tag="VFR00_04895"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013948570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HEU0158623.1"
                     /translation="MDHSSKTPLLDQVVTPDDLRRLPERQLRQLADELRQETIDAVAV
                     TGGHLGAGLGVVELTVALHWIFDTPRDRLIWDVGHQAYPHKILTGRRDRIRTLRQGGG
                     LSGFTKRAESVYDPFGAAHSSTSLSAGLGMAVARDLAGGTHNVVCVIGDGAMSAGMAY
                     EAMNNAGARNERLIVILNDNDMSIAPPVGAISTYLARITSSGTYLHLRDIAKQLAKKL
                     PKYWERRAALAEEMTRTYWTGGTLFEELGFYYLGPIDGHDLNVLIPVLKNVRDAAHGP
                     ILVHVVTKKGKGYPPAEASDDKYHGVNKFNVITGAQVKPKPNAPSYTRVFADSLIAEA
                     RKDDKIVAITAAMPAGTGLDLFAKEFPERTFDVGIAEQHGVTFAAGLATEGFKPFATI
                     YSTFLQRAYDQVVHDVAIQRLPVRFALDRAGLVGADGPTHAGSFDVAYLGCLPGFVLM
                     APADEAELKHMVATAVAIDDRPSALRYPRGEGFGVELPASGEPLEVGRGRIIAEGSTV
                     ALVSFGARLRECVEAANQLAALGLSTTVADARFAKPLDTDLIARLARNHEVLITVEEG
                     SVGGFGSFVLQYLAGEGLFDRGLKVRPMVLPDTFIDHDKPEKMYERAGLNASGIVATV
                     LATLGRETLAKNLAERA"
     gene            3123..3887
                     /locus_tag="VFR00_04900"
     CDS             3123..3887
                     /locus_tag="VFR00_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HEU0158624.1"
                     /translation="MPVTPGRGETGALRLDVALVERRLARSRAQAQDLIKRGLVAVAG
                     SVVRRAATPVRASDAIALAAVAKYVSRGALKLLAALDHFAFPVAGVVALDIGASTGGF
                     TQVLIERGARRVYAVDVGHGQLHPDLKEQSCVVSLEGCDARRLERSIVPEPVAAIVAD
                     ASFISLGKVLPAALALAQPGAWLVALIKPQFEAGHAAVGKGGIVREAAQQQRAVDLVR
                     EWLGGQAGWRVLGVIPAPIRGGSGNQEFLLGAVNDG"
     gene            3877..>4369
                     /locus_tag="VFR00_04905"
     CDS             3877..>4369
                     /locus_tag="VFR00_04905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0158625.1"
                     /translation="MTAEADLQISRLGARGDGIAETPEGPRYLPFALPGERVAGEAGG
                     LPRLQSPPSPERREPHCRHFGVCGGCVAQHMSERLYADWKRSILVEALRQRGLSGDVA
                     PLRPIPAGSRRRAVFTARREKKGGVLLGYHPRESDAVFALQECPVLVAEIVAALPALR
                     EIAA"
BASE COUNT          693 a         1472 c         1455 g          749 t
ORIGIN      
        1 catgggtttt tgcatttttt ccaacgccgc catcgcggcg ctctatgccc gcaagaccta
       61 tggcgtggag cgcgttgcgg tcgtcgattt cgatgtgcat cacggcaatg gcacgcaaga
      121 cgtgttctgg tcggacaaaa atctgctgtt cgcctcgacc catcaaatgc cgctctatcc
      181 cggcaccggg gcgatcagcg aaaccggggt cggcaatatc ttcaatgcgc cgctgcgtcc
      241 gggcgacgac ggcgagcgct tcaaggaggc gttcgaagcg cggctgtcgc cggcgctcaa
      301 gaacttcgcc cccgacctgg tgctgatttc tgccggcttc gatgcccaca aggatgaccc
      361 gttggccaat ctgcggctcc tcgagggcga cttcgcctgg gcgacgggca agatcgccga
      421 gcttgccgcc acccacgccg gcgggcgtct cgtgtccatg ctcgagggcg gctacaactt
      481 gactgcgctc gcccgatcgg tcggtgtcca tgtcaagatg ctgatggagg ccggggcctg
      541 agcgtaggcc gcggcagagc caggagagtt gaggaatgca gacgagcaag cccgcggcca
      601 agcaccttga catcgccggc atgagcttcg agagggcgct caaggagctc gaaagcatcg
      661 ttggccggct cgagcgcggc gacgtcgagc ttgaggaaag cattaccatc tacgagcggg
      721 gtgaggccct gaaggagcac tgtgaccggc tgctgaagca ggcggaagcc agggtggagc
      781 ggctcacctt gtcggagacg ggcacgccca ccggcacgga gcccttcgag cccacgaact
      841 cggcttgacg tgcgtctgcg cgccgccacc tcttggctcg aaacggctta gattctccta
      901 tattgtgggc ctaatgtagg gcccagtacc gccggcgtgt tcggcgtcgc ggcgagcaag
      961 aaggctatcc ccacgcgtgg accattcgag caaaactccg cttttggacc aggtcgtcac
     1021 cccagacgat ctgcgccggt tgcccgaaag gcagctgcgc cagctggccg acgaattgcg
     1081 ccaggaaacc atcgacgcgg tagccgtcac gggcggtcac ctcggcgccg gccttggcgt
     1141 ggtcgagctg acggtggcgc tgcactggat cttcgatacc ccgcgcgatc gcttgatttg
     1201 ggacgtgggc catcaggcct atccgcacaa gatcttgacg ggacggcgcg accgcatccg
     1261 caccctgcgc caaggcggtg gcctgtcggg gttcaccaag cgcgccgaaa gcgtttatga
     1321 cccgttcggg gcggcgcact cctcgacctc gctgtcggcc ggcctcggca tggcggttgc
     1381 ccgcgatctc gccgggggca cgcacaatgt ggtgtgcgtc atcggcgacg gggccatgag
     1441 cgccggcatg gcctacgagg ccatgaacaa cgcgggtgcg cgcaacgaac gcctgatcgt
     1501 gatcctcaac gataacgata tgtcgatcgc cccgccggtc ggcgccatat cgacctacct
     1561 tgcacgcatc acctcgagcg gcacctatct gcatttgcgc gacattgcca agcaactcgc
     1621 caagaagctg ccgaagtatt gggagcgccg cgccgcgctt gccgaggaga tgacgcgcac
     1681 ctattggacc ggcggcaccc tcttcgagga actgggcttt tactaccttg gcccgatcga
     1741 cgggcatgat ctgaacgtgc tgattcccgt gctcaaaaat gtgcgcgacg ccgcgcacgg
     1801 acccattctg gtgcacgtcg tcaccaaaaa gggcaagggc tatcccccgg ctgaggcctc
     1861 ggacgacaag taccacgggg tcaacaagtt caacgtcatc accggcgcgc aggtgaagcc
     1921 caagccgaat gccccgagct acacgcgggt gttcgccgat agcctgatcg cggaggcgcg
     1981 caaagacgac aagatcgtcg ccatcaccgc ggccatgccg gccggaacgg ggctcgacct
     2041 gttcgccaag gaatttcccg agcgcacctt cgatgtgggc atcgccgagc agcacggggt
     2101 gacctttgcc gcgggcctgg ccacggaagg ctttaagcct ttcgccacca tttactcgac
     2161 cttcctgcag cgcgcctacg atcaagtcgt gcacgatgtc gccatccagc gcctgcccgt
     2221 gcgctttgcg ctggatcgcg ccgggttggt cggtgccgac ggccccaccc atgccggctc
     2281 cttcgacgtc gcctaccttg gttgcctgcc tggcttcgtg ctgatggccc ccgccgacga
     2341 ggccgagctc aaacacatgg tggccaccgc ggtcgccatc gacgaccgtc cctcggcgct
     2401 gcgttaccca cgcggggaag gcttcggcgt ggagctgccc gcgagcggcg agccgctcga
     2461 ggtcgggcgc ggccgcatca tcgccgaggg ttcgaccgtg gcgctcgtct cgttcggggc
     2521 gcgcctgcgg gagtgcgtgg aggcggccaa tcagctggcc gcgctcggtc tttcgaccac
     2581 ggtggcggat gctcgatttg ccaaacccct cgacaccgat ttgatcgcgc ggctggccag
     2641 aaaccatgag gtgctgatta ccgtcgagga gggctccgtc ggcggcttcg gctccttcgt
     2701 cctgcagtac ctggcggggg agggcctgtt cgaccgcggt ctcaaggtgc gcccgatggt
     2761 gctgcccgac accttcatcg atcacgacaa gcccgagaag atgtacgagc gggccgggct
     2821 caacgcttcg gggatcgtcg ccaccgtgct ggcgacgctc ggacgagaaa ccctggccaa
     2881 aaatttggcg gagcgcgcct aatcgccgcc ttttccacag gatctccaca gatttcttgg
     2941 cgcgcgggcg ggcgtggatc cgaatccgtg tggacgcccc accgccgccc gcctatgcag
     3001 aaattgtccc tagaccaact ttcctgcttg ccctcgtatc ccccatccgg cagcgggaaa
     3061 aagccctggc gccaacgccg gggctttttt ttccgcaccc gggttgccga cggacgccgc
     3121 ccatgccggt aacgcctggc cgcggcgaga ccggcgcgct ccggctcgac gtcgcgctcg
     3181 ttgaacggcg gctggcgcgc tcgcgggcgc aagcgcagga cctgatcaag cgcggcctcg
     3241 tcgccgtcgc gggcagcgtg gtgcggcgcg ccgccacccc cgtgcgcgct agcgatgcca
     3301 tcgcgctcgc cgccgtggcg aagtacgtct cgcgtggtgc cctcaaattg ctggcggcac
     3361 tcgatcactt cgcttttccc gtggccggcg tcgttgccct cgacatcggc gcctccaccg
     3421 gcggtttcac ccaagtcctg atcgagcgcg gcgcccggcg ggtctatgcc gtcgacgtgg
     3481 ggcatggcca gctgcacccg gacctcaaag agcaatcgtg cgtggtatcg ctcgaaggct
     3541 gcgatgcgcg caggctcgag cgcagcatcg tgcccgagcc ggtggcggcc atcgtcgccg
     3601 atgcgagttt catctcgctg ggcaaggttc tgccggcggc tttggcgctg gcgcaaccgg
     3661 gcgcgtggct cgtcgccctg atcaagccgc agttcgaggc cgggcacgcc gccgtcggca
     3721 agggcggcat cgtgcgcgag gccgcccagc agcagcgcgc ggtcgacctc gtgcgcgaat
     3781 ggctcggcgg gcaagccggc tggcgcgttc tcggcgtcat cccggcgccg atccgaggcg
     3841 gctcgggtaa tcaggaattc ttgctcggag cggtcaatga cggctgaggc ggatctccag
     3901 atctcccggc tcggcgcgcg cggcgatggc atcgccgaga cgcccgaggg gccgcgctac
     3961 ctgccctttg ccttgcccgg cgagcgcgtt gccggcgagg cgggcggcct gccgcgcctg
     4021 cagtcgccgc cgagccccga gcggcgggag ccccactgtc gacattttgg ggtgtgtggg
     4081 ggttgcgtcg cccagcacat gagcgagcgc ctctatgccg attggaaacg ctcgatccta
     4141 gtggaggcct tgcgccagcg aggcttgagc ggcgacgtcg cccccctgcg acccattccc
     4201 gccggctcgc gtcggcgcgc ggtgttcacc gcccggcgcg aaaaaaaagg cggcgtcctt
     4261 ctgggttacc atccgcgcga gagcgatgcc gtgttcgctc tgcaggaatg cccggtgctg
     4321 gtggccgaga tcgtcgcggc tttgccggcc ctgcgcgaga tcgcagcgc
//
LOCUS       DASTMT010000210         6030 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_2079624, whole genome shotgun sequence.
ACCESSION   DASTMT010000210 DASTMT010000000
VERSION     DASTMT010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 6030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6030
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2079624"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..946
                     /locus_tag="VFR00_04910"
     CDS             <1..946
                     /locus_tag="VFR00_04910"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEU0158626.1"
                     /translation="EQADVLVENMAPGTFARLGFDYARLSEINPRLIFAQVKGFAPES
                     PHANYLAFDMIAQAMGGTMAVNGHPDGPPIRPGPTIGDTGTGMLCAIGILGALYQRMA
                     TGRGQHIQIAMRDAMLNYCRTPMSRQSAREDVLPRGGNGVVGTAPGGLYKCAPGGLDD
                     WCYIFASRGNEEHWRRLVHAIGREDLLADPNMSDGLTRAKNQAAVDAAITDWTSKRSK
                     QEVMATIAGAGVPCGAVLNTLELLHDPDLHARGMMQIIQHPVRGPVTVSGWPLRMSDT
                     KVALKCAPMHGADNEAIYGEWLDCSSAEVADLRQRKVI"
     gene            1048..1635
                     /locus_tag="VFR00_04915"
     CDS             1048..1635
                     /locus_tag="VFR00_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012564629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid synthesis family protein"
                     /protein_id="HEU0158627.1"
                     /translation="MDLRKTVTLLEEINSEYGARANPPLRRVAIAAVFKNPLAGKAAG
                     ADLKPLIEASLELGVSLTKSALETLRAKPAELRAYTKAALVGTNGDLEHGAAMIHPRL
                     GMAMRRTIRRGRVIIPGHAKVGPPGTSVDLLFGPLDAGWDLDAVDSTPVLIHDAPRPD
                     EIVLWIGYSTGQRVNARSKGPDQQEVDALIASFGE"
     gene            1895..3058
                     /locus_tag="VFR00_04920"
     CDS             1895..3058
                     /locus_tag="VFR00_04920"
                     /inference="COORDINATES: protein motif:HMM:NF028178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HEU0158628.1"
                     /translation="MRFCVWLLLLICLFFRADAALAQKATKSAKPEASAKGPTADQLL
                     LGQSKNDANLGTVTLMTQRLLAGPLMTAALDLSTLLDEGERFEKMRVLPVIARGKMQN
                     LWDMLYLGGIDLAFLQTDTLEFLKTDPQYASIKDRVRYITVMFSEEICIVARPEIRSL
                     TDLAGKKVSINAKGTAAAVSIPVIFARLGIKADLQYADTNIALERMKSGEFAAHTFLL
                     AKPARAVAELKGDGLHILPIPFSNELADLYLPSKFTNADYPNLIGAGQDVETVAVGNI
                     LAVFNWPEGGERYKKVARFTEAFFGRFAELQKPGFHPKWKDVNLAAQVPGWTRFKPAQ
                     EWLDRAAAQQRAVDADLRTSFGSYLKRRGSSDLGAASESELFQQFLEWRRSKN"
     gene            3161..3922
                     /locus_tag="VFR00_04925"
     CDS             3161..3922
                     /locus_tag="VFR00_04925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0158629.1"
                     /translation="MAIVATVLLPVAAQAQQRPLITLPARILAPASQRTPLAIGVTPE
                     DRLPRNSYLRIRGLPQTVALTEGHAIAPGVWAIPLSVLPVLAAIVPIGAQGTSEIIVD
                     LLSLDGKVLAETKATLIISAADQAAPPAAPPSSARAAPVTPQAGSAALAGPAQAPPNA
                     GDRERALQFYGKGLELLERGDVDAARKFFERAADQGLSQAAMALATTYDPNELAKLKV
                     VGLQGNAAAARKWYDRAAQLGAADANERLRRLGAR"
     gene            complement(4059..5105)
                     /locus_tag="VFR00_04930"
     CDS             complement(4059..5105)
                     /locus_tag="VFR00_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012113460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEU0158630.1"
                     /translation="MERARSWDLPVFAVLVGLGLLAPLVVYPVFLMKVLCFALFACAF
                     NLLIGYVGLLSFGHAMFFGFAAYVAGHLAKVGTVSLPYGLPFLGWRWLVIPIPPLTPE
                     LAILGGVLVAALLGVIVGAIAIRRQGIYFAMVTLALAQMVFFLCVQAKFTGGEDGIQA
                     IPRRPILGALDVGNDFTFYYVVLAIFCAGFLIIYRTIHSPFGQVLKAIRENEPRAVSL
                     GYAVSRFKLIAFVISAALAGLAGATKAMVFQLASLTDVTWQMSGEVVLMTLVGGMGTV
                     FGPAVGAAIIIYMQNYLAGFGEWVLVIQGVIFVVAVLVFRRGIVGELVAFMRRRTTKP
                     AEPAAAIGLAKPAA"
     gene            complement(5119..6000)
                     /locus_tag="VFR00_04935"
     CDS             complement(5119..6000)
                     /locus_tag="VFR00_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012113459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEU0158631.1"
                     /translation="MFELIGVPPQALFGQLLLGLINGAFYALLSLGLAVIFGLMNVIN
                     FTHGAQYMMGAFCAWMLLTYVGIPYWGALVLAPIIIGFTGVLIERLFISRLYHLDHLY
                     GLLLTFGLALIVEGLFRREYGISGLPYSNPIPGGTNLGFMFLPWYRAWVVVFSLTVCL
                     ATWFVIEKTQLGAYLRAATERPDLVQAFGINVPRMITLTYGFGVGLEALAGVLAAPIY
                     SVNPVMGANLIIVVFAVVVIGGMGSIMGSIITGFGLGVIEGLTKVFYPEASNTVIFII
                     MAVVLMLRPQGLFGTPR"
BASE COUNT         1172 a         1989 c         1937 g          932 t
ORIGIN      
        1 cgagcaggcc gacgtgctcg tcgagaatat ggcgcccggc acctttgcgc gtttggggtt
       61 cgattacgcc cggctcagcg agatcaatcc gcgcctgatc tttgcgcagg tcaaaggctt
      121 cgcgccggaa agcccgcacg cgaactattt ggcctttgac atgatcgccc aggcgatggg
      181 cggcaccatg gccgtgaacg gacacccgga cggtcctccc attcgccccg ggcccacgat
      241 cggcgatacc ggaacgggca tgctctgcgc cataggcatt ctcggcgcgc tctatcaacg
      301 catggcgacc ggtcgcggcc agcacatcca gatcgccatg cgcgacgcga tgctcaacta
      361 ctgccgcacg cccatgagcc gccagtcggc gcgtgaagac gtgctgccgc gcggcggcaa
      421 tggtgtggtc ggcaccgcgc cgggtggcct ttataaatgc gcgccaggcg ggctcgatga
      481 ttggtgctac atttttgctt cgaggggaaa cgaggagcat tggcggcggc tcgtacacgc
      541 gatcgggcgt gaagatctgc tggcagatcc caacatgtcc gacggcctca cccgcgccaa
      601 gaaccaggcg gcggtcgatg ccgcgatcac agactggacg tcgaaacgta gcaagcagga
      661 ggtgatggca accattgccg gcgcgggggt accctgcggc gccgtgctca acacgctgga
      721 gctgctgcac gacccggatc tgcatgcccg cggcatgatg caaatcatcc agcatcccgt
      781 gcgcggaccg gtgacggtgt cgggctggcc cttgcgcatg tccgatacca aggtcgcgct
      841 caaatgcgcg ccgatgcatg gcgccgacaa cgaggcgatt tatggcgagt ggctcgactg
      901 ctcgtccgcc gaggtcgccg atctgcgcca acgcaaggtg atttgatggc ccgttcgctc
      961 cgccacgcag cgcctaggtg tggttgcgcg gggtggggcg aggtttacgg gagtgcaacg
     1021 aggtcgccgg atacaaggag ccgagccatg gacctgcgca agacggtcac gcttctcgag
     1081 gagatcaaca gcgagtacgg cgcgcgcgcc aatccaccgc tgcggcgcgt ggcgatcgcc
     1141 gccgtgttca agaacccgct ggcgggcaag gccgccggcg cggatttgaa acccctgatc
     1201 gaggcctcac tcgaactcgg cgtttccctc accaaatcag cgctcgagac gctgagggcc
     1261 aagcccgccg agctccgggc ctacaccaag gccgccctgg tcggcaccaa cggcgacctg
     1321 gagcatggcg cggccatgat ccacccccgc ctcggtatgg ccatgcggcg cactattcgg
     1381 cgtggccgcg tgatcatccc cgggcacgcc aaggtgggcc cgcccggcac gagcgtcgat
     1441 ctcctgttcg gcccgctcga tgccggctgg gatctcgatg ccgtcgattc cacgccggtg
     1501 ctgatccacg atgcgccgcg ccctgacgag atcgtgctgt ggatcggcta cagcaccggc
     1561 cagcgcgtca atgccaggag caaaggcccc gaccagcagg aggtcgacgc cctgatcgcc
     1621 tcgttcggcg agtagcccga aatcgcaggc gggtttgtga tgttgacaca actcgtgctc
     1681 cgtcgtcgga ccggtcgtgc gcggagctta tcatcagctt gacacaatcg ctgcctagcg
     1741 tagcggcaat actaaaaatg attgcgcggc tccaaccacg cacgttgcct aacagatcga
     1801 tggccggctc cgcgggagcg ccggtggaag tgcatggacg tgcgctcgtt cgcgcgtgcc
     1861 tcgagcgtcc gacgcgccct cggaggagga aacggtgcga ttctgtgttt ggctcctgct
     1921 gctgatttgc cttttctttc gcgccgatgc ggccttggcg caaaaggcca ccaagtccgc
     1981 caagcccgaa gcctctgcca aaggcccgac ggccgaccag cttctgctcg gccaatccaa
     2041 gaacgatgcc aatctcggca ccgtgacgct gatgacccag cggctgttgg ccgggccgct
     2101 gatgacggct gcgctcgatc tttccacgct gctcgacgaa ggcgagcgct tcgagaaaat
     2161 gcgtgtgctg cccgtgattg cccgcggcaa gatgcaaaac ctctgggaca tgctttatct
     2221 gggcggcatc gatctggcat tcctgcagac cgacacgctc gagttcctga agaccgatcc
     2281 gcagtacgcg agcatcaagg accgcgtgcg ctacatcacg gtcatgttct ccgaggagat
     2341 ttgcatcgtc gcccgaccag agatcaggag cctcacggac ctcgccggca agaaggtcag
     2401 catcaacgcc aagggcacgg ccgccgcggt cagcatcccc gtcatcttcg cgcggctcgg
     2461 catcaaggcg gacctgcagt atgccgacac caatattgcg ctcgagcgca tgaagagcgg
     2521 cgagttcgcc gcgcacacat ttcttctcgc caagccggcc cgggcggtcg cggagctcaa
     2581 gggcgacggt ttgcacatcc tgcccatccc gttttccaac gagttggccg acctttacct
     2641 gccctcgaaa ttcacgaacg cggattatcc caacctcatc ggcgccggcc aggacgtgga
     2701 aaccgtcgcg gtcggcaaca tcctcgccgt cttcaactgg cccgagggtg gtgagcgcta
     2761 caaaaaagtt gcgcgcttta ccgaggcctt ctttggccgg ttcgccgagc tgcagaagcc
     2821 gggcttccac cccaagtgga aggacgtgaa cctggcggcg caggtgccgg gctggacccg
     2881 gttcaaaccg gcgcaggagt ggctcgacag ggcggccgcg cagcagcgcg cggtcgacgc
     2941 cgatctcagg actagcttcg gcagttatct gaagcggcgc ggcagcagcg atctcggcgc
     3001 cgcgtcagaa agcgagctgt tccaacagtt cctggaatgg cgccgcagca agaattgagg
     3061 caacgggttt gaggggaggc gtgccctgcg caagagctgg agacgatcgt ggcttggccg
     3121 tcccggcccg cagccgagcg cgcgcgaggt atggcttggg ttggccatcg tcgccacggt
     3181 gctgctgccc gttgccgccc aggctcaaca gcgaccgctg atcacgctcc cggcccggat
     3241 tctcgcgccc gcctcgcaac gcacgccgct ggcgatcggg gtcactcccg aggacaggct
     3301 gccgcgcaat tcctacctgc gcattcgcgg cctgcctcag accgtcgcgc tcaccgaggg
     3361 gcacgcgatc gccccggggg tgtgggcgat tccgctatcg gtcctgccgg ttcttgcggc
     3421 catcgtgccg atcggcgcgc agggcacctc cgagatcatc gttgatcttt tgagcctaga
     3481 cggcaaggtg ctggcggaaa ccaaggcgac gctgatcatc tcggcggcgg atcaggcagc
     3541 cccgcctgca gcaccgccat cctccgcccg cgccgcgccg gtcacaccgc aagctggctc
     3601 cgcggcgctg gcaggcccgg cccaggcccc gccgaatgca ggcgaccgcg aacgcgcgct
     3661 gcaattctac ggcaagggct tggaattgct cgagcgcggc gacgtcgatg cggcgcgcaa
     3721 gtttttcgag cgcgccgccg atcaggggct ctcccaagcc gccatggcgc tcgccaccac
     3781 ctatgatccc aacgagctag ccaagctcaa agtcgtgggc ctacagggca atgccgccgc
     3841 cgcccgcaag tggtatgacc gggcagccca gcttggcgcc gccgacgcca acgagcgctt
     3901 gcgccgcctc ggcgcgcgct agtcgcatcg ccgtcgcttc gatgccgtcc caactgcgcc
     3961 ccggccgtag ctgacgcacg ccggttgctg ctgcgctgcg tttcgctaga gcgacgcacc
     4021 tcgatcccgc gggcaggcgg ctcgcggcct tccctcggct atgccgcggg tttggcgagc
     4081 ccgatcgccg cggcgggctc ggccggcttg gtggtgcgcc gccgcataaa ggcaaccagt
     4141 tcgccgacga tgccgcgccg gaagaccaac accgccacca caaagatcac gccctggatc
     4201 accaacaccc attcgccgaa gcccgccagg tagttttgca tgtagataat gatggcggcc
     4261 ccgacggcag gaccaaagac ggtgcccatg ccgccgacca acgtcatcag cacgacctca
     4321 cccgacattt gccaggtcac atccgtcaag gaggcaagct ggaacaccat ggccttggtt
     4381 gcccccgcaa ggccagccag ggccgccgag atcacgaagg cgatcagctt gaagcgactg
     4441 acggcatagc cgagcgaaac ggcacgcggc tcgttttcgc gaatggcctt gagcacctgg
     4501 ccgaacgggg aatggatcgt gcgatagatg atcaggaagc cggcgcagaa gatcgccagc
     4561 acgacatagt agaaggtaaa atcgttgccg acatcgagag ccccgaggat cggccggcgc
     4621 ggaatggcct ggatcccgtc ctcgcccccc gtgaacttgg cctgcacgca caggaagaac
     4681 accatctgcg ccagggcgag tgtcaccatg gcgaagtaga tgccctggcg gcgaatggcg
     4741 atggccccga cgatgacgcc gagcagcgcc gccaccagca cgccgccgag gatggcaagc
     4801 tccggggtga gcggcggtat cgggatgacg agccacctcc agcccagaaa cggcaagccg
     4861 tagggcaggc tcacggtgcc gaccttggcg aggtgtccgg ccacgtaggc agcgaagccg
     4921 aagaacatgg cgtggccgaa ggagagcagc cccacgtaac cgatcagcag gttgaaggcg
     4981 caggcgaaga gcgcgaagca caagaccttc atcaggaaca cggggtagac gacgaggggt
     5041 gccagcaagc caaggccgac gaggaccgca aacaccggca agtcccagct gcgtgcgcgt
     5101 tccatggtca tagcttcgtc atctcggagt tccgaacagg ccctggggcc gcagcatcag
     5161 caccaccgcc atgatgatga agatcaccgt gttggaggcc tcgggataga acactttggt
     5221 cagcccctcg atcacgccca agccgaagcc ggtgatgatc gatcccatga tcgagcccat
     5281 gcccccgatc accaccacgg cgaacaccac gatgatgagg ttggcaccca tcacgggatt
     5341 gacggagtag atcggcgccg ccagcacgcc cgccagcgcc tccaggccca cgccaaagcc
     5401 ataggtcagg gtgatcatgc gcggcacgtt gatgccgaac gcctgcacca agtccgggcg
     5461 ctcggtggcg gcgcgcaagt aggcgccgag ctgcgtcttc tcgatgacga accaggtggc
     5521 aaggcaaacc gtaagcgaga agacgacgac ccaggcccgg taccagggca gaaacataaa
     5581 gccgagattg gtgccgccgg ggatgggatt ggagtagggc agccccgaaa tcccgtactc
     5641 gcgccggaac agtccctcca cgatcaaggc caggccgaac gtcaggagca ggccgtagag
     5701 gtgatcgagg tgatagagcc gcgagatgaa gagccgctcg atgaggacgc cggtgaaccc
     5761 gatgataata ggagccaaaa ccagggcgcc ccaataggga atgccgacat aggtgagcag
     5821 catccaggca caaaaggctc ccatcatgta ttgggcgcca tgcgtgaagt tgatcacgtt
     5881 catgaggcca aagatgacgg cgaggccgag cgacaacagg gcgtagaacg ccccgttgat
     5941 gagaccgagc agcagctgcc cgaacagcgc ctgcggcggc acgccgatca gctcgaacat
     6001 ggcgcagtcg ttctcttctc aaacgcttga
//
LOCUS       DASTMT010000211        12634 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_440572, whole genome shotgun sequence.
ACCESSION   DASTMT010000211 DASTMT010000000
VERSION     DASTMT010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 12634)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12634)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12634
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_440572"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(117..1145)
                     /locus_tag="VFR00_04940"
     CDS             complement(117..1145)
                     /locus_tag="VFR00_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019402753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEU0158632.1"
                     /translation="MSNMAFTRRRLGGTSVLTSALGFGGAPIGGFRFALSDRQGVEAL
                     GEAFDGGIRYFDTSPYYGYGRSELIFGYALRPHPRDDYVLSSKVGRYMTPLAPGESVA
                     GWRPGGLPFKATFDYSREGTLRSLEQSLLRLGLARLDIALIHDADVHTHGSQAEADRR
                     FAEAMAGSYPALVELRRGGVIKAIGVGLNETAMSLRFAHEADLDCIMLAGRYTLLEQG
                     ALDALLPLCEKKNMSILLAAPLNSGILATGARAGATYDYKPAPPEILDKVRRIEEVCR
                     RHNVDLTTAALQFPLAHPRLASIVVGALTADEVRENIARLAAPVPSDLWRELKHEKLL
                     AEAAPVPA"
     gene            complement(1238..2464)
                     /locus_tag="VFR00_04945"
     CDS             complement(1238..2464)
                     /locus_tag="VFR00_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002970137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEU0158633.1"
                     /translation="MPAWEPRYARRAERMVASEIRELLKVLERPNIVSFAGGIPDPAL
                     FPVAEIASAYAEVLSGSGSAGAGLQYSVSEGYAPLRQWIAGYMGRLGVPCGQDNIVIT
                     NGAQQALEFLARLLLSAGDTALVTMPTYLGALQAFAAYEPRYDELRPEQGNRTPQSYL
                     AAAAAGGGAVKFAYVVPDFANPTGATLSSAAREGLLDLAERLDIPILEDAAYSALSFE
                     GGPRASILARDIQRCGSIEAARTFYCGTFSKTLSPGLRVGWIVGPRALIRRLVLIKQA
                     CDLNSASLNQMVIHRVAETVFERQVAAARAHYRRRRDRLLAALAREMPKGCHWSEPEG
                     GLFVWLRLPHGLDSACLLGRAVEEAGVAFVPGAAFYFDRSGHDHLRLSYSLASEAEID
                     AGMARLAALVRAMAPS"
     gene            complement(2643..3923)
                     /gene="glp"
                     /locus_tag="VFR00_04950"
     CDS             complement(2643..3923)
                     /gene="glp"
                     /locus_tag="VFR00_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006935081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HEU0158634.1"
                     /translation="MDKKRLDDCFLSDRARLRHDEALAILRARIGAVVGRECLALPAA
                     LNRVLAEAAIAPQNVPAHTNAAVDGYSFAAADYRLDAATTLALSGRAAAGHPRAGVPP
                     PGSAVRIFTGAVMPAGHDTVAMQEDARTAVENGREVVTIPSGLKPQANVRKAGEDVKA
                     GETVLGPGAVLRPQDLGALAALGLAEVTCFARLKVALLASGDEIVRPPSGLTAGQVYD
                     ANTPMLLGLIAGAGATAVDLGVLPDDAATVRRRLAEAAAGYDVIITSGGASLGEEDHM
                     VAALDALGKRHLWQLAIKPGRPMTFGQIGDAVVVGLPGNPVAVFVCFLLYVWPLLRRL
                     GGAPWPEPRRFLLPARFDFPARKIGRREFWRGSLVAEPQGLAVAKFPRDGSGLISGLR
                     AADGLIEIPEDVSGVAWGDPVAFLPFSEYGIGAR"
     gene            complement(4056..5525)
                     /locus_tag="VFR00_04955"
     CDS             complement(4056..5525)
                     /locus_tag="VFR00_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004684894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M81 family metallopeptidase"
                     /protein_id="HEU0158635.1"
                     /translation="MRLFAATLATETNTFSPLPTSLASYREGVFLRPGEHPDDAPRMC
                     TAPLFVARQRAAKESFSLIEGSCFAASPAGTTNREDYELMRDEILDQLRRALPLDGVL
                     LGLHGAMVAYGYDDVEGDILERVRAIVSPGCVIGVELDPHCHLTLKRLQLSDVIVLYK
                     EFPHTDVVERAEDVLDLVLAQLRGQIKPVMSVYDCRQIQSYPTTLPLMRGFVDKIKAL
                     EGKDGILSISIGHCFPYADVPELSARVLVIADGAKTKADALARELGEELVAMRGKTLP
                     EYLDIDAAITAGLAGRDGPVVLAEPADNAGGGAPSDNTSILRALVARDVPNAGIGPIW
                     DPVAVRLAFDAGIGASFALRLGGKTGPASGQPFDAEVTVLGLARDAWQSFGPTKVPVG
                     DCAAVRIGGVDVVLISNRTQALGLELFRNVGIEPTRKRLLVVKSTNHFMAAFGPIATK
                     VLYVDSDAPLARDYRRVPYRRVQRPIWPLDEVTHPGLIY"
     gene            5793..8123
                     /locus_tag="VFR00_04960"
     CDS             5793..8123
                     /locus_tag="VFR00_04960"
                     /inference="COORDINATES: protein motif:HMM:NF025214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU0158636.1"
                     /translation="MSKPSPTAPPALSKGEAIRRAMALHRSGKLEQAAQLYRAVLAVA
                     PNDFDALHLLGVLLSAARPAGEGIELIRRALTLEPNFAGAHNNLGTALLAAKRPQEAL
                     KSFQRAVELKADFAEAHSNLGNALADLGRREEALASHQHAIALKPDYAEAYNNLGNVL
                     IALGRSDEAARACRRALEIRPNYAKAHNNLGTALATQHRAEDAVACFRQAIAVNANFA
                     DAYRGLGEALLALRREDEAVAALERAVDLRPDEADAHVSLGSALSARRQMGRAAAAYR
                     RALELKPENGVAHHNLGNALAAAGDLDAAAASYRLAVEHMGEDCHALLQYVQVCHQMC
                     AWQQAAPQAQALLERVGRPSFNAPPFPLLATVDDPVLQHKAARAYVSHAIGSGYPALW
                     KGERYGHPRLKLAYFSFDLREHPAARLVVDLIERHHRGQFEVFAISSGPDDKSALRRR
                     VSQACDHFIDVSQLADAAIAEKLRALEIDVVVDLDGHTEGSRSRALAWRPVPAQVAYL
                     GYPATMGADFIDYAIVDRCIVPDDQHPRFSEKLVYLPDCFQVSDSKRPLPAQAEPRAK
                     LGLPEDAFVFACFNNTYKITPAVFEVWMRLLGAVPHSVLWLVADNRWADANLKEAARQ
                     NQIDPARLIFAPRVEYASFLKRQLAADLFLDTAPYNGGATCNDALWAGLPVVTCCGRA
                     YAARMAASLLQGIGMPELVTTSLEHYEALALALARDQAELTRLRRKLVEARPQSALFD
                     TERFCRNLEAAFAEMRRVSEAGEQPSSFSIETAASP"
     gene            8200..9882
                     /locus_tag="VFR00_04965"
     CDS             8200..9882
                     /locus_tag="VFR00_04965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020094918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEU0158637.1"
                     /translation="MSIEMRGADIVVRSLERAGCTNVFTLSGNHIMPLFDAVLGSKLR
                     LLHVRHEAAAVHMADAWGRLTGQCGIALVSGGAGHANALGALATAAAADSPLVLLSGH
                     AGLNEVGRGAFQEMRQADMAAPVTKAAWVAQQPAELGHDIARAMRTALSGRPGPVHVS
                     LPFDLLQARVPPSPDLVPGREAFGAEPMPFAAAAAEATLRHLFAARRPLILGGPRLCQ
                     RDGPALLGELGAATGVPSFGMESPRGLADPRLGALAQALGRADLIVLLGKALDFTLRF
                     GDQPHVAADCRFIGIDPDPGQLARLSRSMGDRVLVQVSADPVAAAASLRTRCGPRGTK
                     NAWAEEVTWALSVRPQASTFAAAGPGKLHPIELCRALAPYLERNRDAILICDGGEIGQ
                     WPQAALSPPRRLINGVAGAIGAAVPFALAARLAEPKAPVLAVMGDGSFGFHMAEFDTA
                     VRYDLPFIAVVGNDATWNAEHQIQLRDYGAERTHACGLLPSRYDLVALALGGHGELVT
                     AADALPAALERALAAGKPACLNVMIERVPAPTLPARAPLQAPDAGASDKVTS"
     gene            9944..10195
                     /locus_tag="VFR00_04970"
     CDS             9944..10195
                     /locus_tag="VFR00_04970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0158638.1"
                     /translation="MRASIILGAGILLAVTFAAQAQSSRQQPRQPPQTPPAVIIERDR
                     LQPPPAPERRVVPPRTFQDISPPMQGPTPLSPMAPRIGN"
     gene            complement(10223..11626)
                     /gene="lpdA"
                     /locus_tag="VFR00_04975"
     CDS             complement(10223..11626)
                     /gene="lpdA"
                     /locus_tag="VFR00_04975"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEU0158639.1"
                     /translation="MAGSYDLVVIGTGPGGYVCAIRAAQLGLKVAVIEKRATHGGTCL
                     NIGCIPSKALLHASERYEEARHHLAGMGIEVVPKLNLASMLAFKDQGVKGNVDGVAFL
                     LKKNKIDIFHGLGTLVAPGRVKVARDGAAPEQLEARSIVIATGSDVARLPGVEIDEAS
                     VVSSTGALTLPQVPKRLLIIGAGVIGLELGSVWRRLGSEVLTVEFLDRILPGTDAEVA
                     RGLQRLLMKQGMSFKLSSKVTRIAKNGAQQKVTIEPAAGGAPIGVEADVVLIAVGRVP
                     YTDGLGLEAVGVQLDNKKRIVVDARYQTNVAGVYAIGDVIAGPMLAHKAEEEGIAVAE
                     TLAGQAGHVNYDAIPNVIYTFPEVASVGKSEEELKAANVAYKVGKYPFTANARAKVNK
                     TTDGFVKILADAGSDRILGVHIIAAAASEMIAEATAMLEFGGSAEDLARTCHAHPTLT
                     EAVKEAALAVDKRAIHA"
     gene            complement(11630..>12634)
                     /gene="odhB"
                     /locus_tag="VFR00_04980"
     CDS             complement(11630..>12634)
                     /gene="odhB"
                     /locus_tag="VFR00_04980"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018012726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HEU0158640.1"
                     /translation="KPQPAAPAPRAAERPAPADEPASDGAMPPAPAARKIMAEQDLSA
                     AEVAGTGRRGQILKEDAQAALARRAAGPATTSAAGSYLPASVPPATQALRAPSGPNDA
                     TREERVRMTRLRQTIARRLKEAQNTAAMLTTFNDVDMSAITELRRAYKELFEKRYRTK
                     LGFMAFFVKACVQALKEVPAVNAEIDGDEIIYKNYYHIGVAVGTDRGLVVPVLREADR
                     LSFAEIEQAIGEFGRRAREGKLGIEEMQGGTFTISNGGVYGSLLSTPILNAPQSGILG
                     MHRIEERAVVRNGQIVARPIMNLALSYDHRLVDGREAVTFLVRVKEFLEDPQRLVLEL
                     "
BASE COUNT         2104 a         4397 c         4103 g         2030 t
ORIGIN      
        1 gaccgatgct ggaaacggag ctgggtccgc tcgagcggct cggcagcgaa caggcggcct
       61 gaggccggcc agcaacgcgc ggcatcgcgc gcgggctgac gcccggcgcg atcgcctcag
      121 gccggcacgg gcgcggcctc ggccagcaat ttttcgtgct tcagctcgcg ccacaaatcc
      181 gaggggaccg gagcggcgag gcgcgcgata ttttcgcgca cttcatccgc cgtgagcgcg
      241 cccacgacaa tcgaggccag gcgcggatgg gccaacggaa actgcagcgc ggcggtggtg
      301 agatcgacgt tgtggcgccg gcagacctcc tcgatgcgcc gcactttatc caggatctcc
      361 ggcggggcag gcttatagtc ataggtggca ccggcgcgcg cgccggtggc gagaattccg
      421 gaattgagcg gcgccgctag caagatcgac atgttttttt tctcgcacag aggcaacagc
      481 gcatcgagcg ctccctgctc caatagggtg tagcgccccg ccagcatgat gcagtcgaga
      541 tcggcctcgt gggcgaagcg caagctcatg gcggtttcat tgaggccgac gccgatggcc
      601 ttgatcacgc cgccgcgccg gagctccaca agcgcgggat aactgcccgc cattgcctcc
      661 gcaaagcgcc gatcggcttc ggcctgactg ccgtgcgtat gcacatcagc gtcgtggatc
      721 agggcgatat cgagccgcgc caggcccaag cgcagcagcg actgttcgag cgagcgcagg
      781 gtaccctcgc ggctgtaatc gaaggtcgcc ttgaacggca gcccgccggg acgccagccc
      841 gccacgctct cgccgggggc aagcggcgtc atgtagcggc cgaccttgct ggagagcacg
      901 tagtcatcgc gcgggtgcgg gcgcaacgca tagccgaaaa tgagctcgct gcggccgtag
      961 ccgtaatagg gcgaggtgtc gaaatagcga atgccaccgt cgaaggcttc gcccagcgcc
     1021 tccaccccct gccgatcgct cagcgcgaag cgaaagccgc cgatcggggc cccgccaaag
     1081 ccgagcgcac tcgtcaacac cgaggtgccg cccagacgac gccgggtaaa cgccatgttg
     1141 ctcatcgctc cctctccttg ctcttccctc cccgctcccg cgagcggcag acctgtgagg
     1201 ggattgcgag ggatggttgc gaccggcggg cccgctgtca actcggggcc atcgcgcgaa
     1261 cgagcgccgc cagccgggcc atgccggcgt cgatctccgc ctcactggcg agcgagtagg
     1321 agaggcgaag gtggtcgtga ccactgcgat caaaatagaa ggcggctccg ggcacgaagg
     1381 caaccccggc ctcctcgaca gcgcgcccga gcaggcaagc gctgtccaac ccgtgcggca
     1441 ggcgcaacca tacgaacagg ccgccctcag gttcgctcca gtggcacccc ttcggcatct
     1501 cgcgcgcgag cgccgccagc aggcgatcgc gtcggcgtcg atagtgcgcg cgcgccgccg
     1561 cgacctggcg ctcgaacacc gtctcggcca cgcggtgaat caccatctga ttgaggctgg
     1621 cgctgttgag gtcacaggcc tgcttgatca gcaccagacg ccggatcaag gcgcgcggcc
     1681 cgacgatcca accgacccgc agaccgggcg acagggtttt ggaaaatgtg ccgcagtaaa
     1741 acgtgcgcgc cgcctcgatg ctgccgcagc gttgaatgtc gcgagccagg atcgaggctc
     1801 tcggcccgcc ctcgaagctc agcgcgctat aggctgcgtc ctcgagaatg ggaatgtcga
     1861 gccgctcagc caaatcgagc aggccttcgc gtgccgcgga cgatagcgtg gcgccggtgg
     1921 ggttggcgaa atcgggcacg acataggcaa attttaccgc ccccccgccg gcagcagccg
     1981 ccgcgagata ggactgcggg gtccggtttc cctgctcggg tctcagttca tcataacgcg
     2041 gctcgtaggc ggcgaacgcc tgcagcgcgc cgaggtaggt cggcattgtc acgagcgccg
     2101 tgtcgccggc cgacagcagc aggcgagcca ggaactccag agcctgctgg gcgccgttgg
     2161 tgatgacgat gttgtcctgg ccgcagggca cgccgaggcg acccatatag cccgcgatcc
     2221 actgccgcag cggcgcgtag ccctcgctca ccgagtattg caaaccggcg cccgcagacc
     2281 cggagcccga gagcacctcg gcataggcgc tggcgatctc ggccacggga aaaagggcag
     2341 gatcgggaat gccgccggcg aaggagacga tgttgggccg ctcgagcacc ttgaggagct
     2401 cgcgaatttc ggaagcgacc atgcgctcgg cgcgacgcgc atagcgcggc tcccaggccg
     2461 gcatttgccc gctgatgctc atggctggct cccggttttt cgctccgggc attaatatgt
     2521 cagtaatact gacctattcg caagcgagca aataagcgct tgctgcagcg cacagataag
     2581 ctcttgatta ggaattgttt ttcgccgcat tccccgcgcc ggcacggccc gcccgcggcc
     2641 gctcagcgcg cgccaatgcc gtattcggaa aagggcaaga aggcgaccgg gtcaccccag
     2701 gccacccccg acacgtcctc cgggatctcg atcaggccgt cggcggcgcg cagacccgag
     2761 atcaagcccg acccgtcgcg tggaaacttg gccacggcca atccctgcgg ctccgccacc
     2821 aggctgcccc gccagaattc acggcgcccg atcttgcggg cgggaaaatc gaagcgcgcc
     2881 ggcaacagaa aacggcgtgg ctcgggccag ggcgcgccgc ccaaacggcg cagcaaaggc
     2941 cacacgtaga gcagaaagca gacaaatacg gcgaccggat tgcccggcag gccgacgacg
     3001 acggcatcgc cgatctgacc aaaggtcatg ggacgcccgg gtttgatggc aagctgccag
     3061 aggtgccgct taccgagcgc gtcgagtgcg gccaccatgt ggtcttcctc gccaaggctg
     3121 gcgccgcccg aggtgatgat gacgtcgtag cccgcggcag cttcggccag gcgccggcgc
     3181 accgtggccg catcgtccgg caacacgccg agatcgacgg ctgtcgcgcc ggcgccggca
     3241 atgaggccca gcaacatggg cgtgttggca tcgtagacct ggcccgccgt cagcccgctt
     3301 ggtggccgca cgatctcatc cccactggcg aggagcgcga ctttgaggcg cgcaaagcag
     3361 gtgacctcgg ccaggcccaa ggcggcgagc gcacccaagt cctgcggtct gagcaccgcg
     3421 cccgggccga gcacggtttc accggctttg acatcctccc ccgccttgcg cacgttggcc
     3481 tgcggcttga gcccgcttgg aatggtcacc acctcccgcc cgttctcgac cgcggtgcgt
     3541 gcgtcctctt gcatcgccac ggtgtcgtgc cctgcgggca tgaccgcgcc cgtgaaaatg
     3601 cgcaccgcgc tgcctggcgg cggcacgccg gcacgcgggt gccccgccgc ggcccgcccg
     3661 ctcagcgcca gcgtggtcgc cgcgtccaag cggtaatcgg cggccgcaaa ggaatagccg
     3721 tcgacggcgg cattggtgtg cgcgggcacg ttctggggcg cgatcgccgc ttcagccagc
     3781 acccggttca aggccgcggg cagcgctagg cactcgcggc ccaccaccgc gccgatgcgt
     3841 gcgcgcagga tcgcgagcgc ctcgtcgtga cgcaggcgag cgcggtcgct cagaaagcag
     3901 tcatcgagcc gctttttgtc catgcggtcg tgccgggtct tgtcatcttg cccgaggcca
     3961 cgcctgcccc ggcgcgctct ccttaaaact actctagcga aaaaagcgcg gccggaccat
     4021 gcggcgggcc tcgccccgcc ggcgcccatg cgaactcagt agatcaggcc cggatgggtg
     4081 acctcatcga gcggccagat gggacgctga acgcgacgat aggggacgcg gcgatagtcg
     4141 cgcgcgagcg gggcatcgct atcgacataa agcacctttg tcgcaatcgg cccgaacgcc
     4201 gccatgaaat ggttggtcga tttgacgacc aacagccgct tgcgcgtcgg ctcgatgcca
     4261 acattgcgga agagctcgag gcccaaggcc tgcgtgcggt tgctgatcag tacgacatcg
     4321 acaccgccga tccgcacggc ggcacaatcg ccgacgggaa ccttggtggg tccgaagctt
     4381 tgccaggcgt cccgggcaag gcccagaacg gtcacctcgg catcgaacgg ctggccggaa
     4441 gccgggccgg tcttgccgcc caggcgcagg gcaaagctgg cgccgatccc cgcgtcaaag
     4501 gcgagcctaa cggcgaccgg atcccaaatg gggccgatgc ctgcattcgg cacgtcgcgc
     4561 gccacaagcg cgcgcaggat gctggtattg tcggacgggg cgccaccgcc ggcgttatcg
     4621 gccggctcgg ccagcaccac cggcccgtcc ctgccagcaa ggccggccgt gatggcggcg
     4681 tcgatgtcga ggtactcggg cagcgtcttg ccgcgcatgg ctaccaactc ctcgcccagt
     4741 tcacgcgcca gggcatcggc cttggtcttg gcaccgtccg cgatcacgag cacgcgcgcg
     4801 gagagttcgg gcacgtccgc atagggaaag caatgaccga ttgagatgga cagaatgcca
     4861 tccttgccct ccagcgcctt gatcttatcg acaaagccgc gcatcagcgg cagcgtcgtc
     4921 ggatagctct ggatttgccg gcaatcgtaa accgacatga ccggcttaat ttggccgcgc
     4981 agctgcgcca gcaccagatc gagcacgtcc tcggcgcgtt cgaccacatc ggtgtgggga
     5041 aactccttgt acagaacgat aacgtcggaa agttgcaggc gtttcaaagt gagatggcaa
     5101 tgcggatcga gctcgacgcc gatgacgcag ccgggagaaa cgatcgcgcg cacgcgctct
     5161 aggatgtcgc cttcgacgtc gtcgtagccg taagccacca tggcaccgtg cagacccagc
     5221 agcaccccgt cgagcggtag cgcgcggcgc agctggtcga ggatttcatc gcgcatcagc
     5281 tcgtagtcct ctcggttggt cgtgccggcc ggactggcag caaaacaact gccctcgatc
     5341 aggctgaaac tctccttggc ggcgcgctgg cgcgccacga acagaggcgc ggtgcacatg
     5401 cgcggtgcgt catcggggtg ctccccggga cgcaggaaca cgccctcccg ataggaggca
     5461 aggctggtcg gtagcggcga gaaggtattg gtctcggtcg caagggtagc cgcaaacagg
     5521 cgcatggacg ggctctccgc tcggggtgca actgacaaac gcgtttgcgg tcttgttggg
     5581 acattcggcc tcgagccgtc aaggtcaggc cgcatggggc cgcaatcccc cggcgggccg
     5641 caggggccgc tcccctcacc cgcgccccag cggtctgccc gcaggaaagc gcattttgct
     5701 cgccgtgccg gcgccgaggc tgataaaacc gctcgccgcg cgcgccgcgc gtgcgcgccc
     5761 cgcattttga gaccagcccg gaacttcctc gcgtgagcaa gccttccccg acagccccgc
     5821 cggcgctcag caagggcgag gccattcgcc gagccatggc gctgcatcgc agcggcaaac
     5881 tcgagcaggc ggcccagctt taccgggccg tcctcgccgt cgctcccaac gacttcgacg
     5941 ctctgcatct tctgggcgtg ctgttgagcg cggctaggcc agccggcgag ggcatcgagc
     6001 tgatccgacg ggcgctcacg ctcgagccca attttgccgg cgcgcacaat aatctgggga
     6061 ccgcgctgct ggcggcgaaa cgaccgcagg aggcgctcaa gagcttccag cgtgccgtcg
     6121 aactcaaggc tgatttcgcc gaggcgcata gcaacctcgg caatgcgctg gccgacctcg
     6181 gtcggcgcga ggaagccctc gccagccatc agcacgcgat cgcgctaaaa cccgactacg
     6241 cggaagccta caacaatttg ggcaatgtcc tgatcgcact cggtcgctca gatgaggcgg
     6301 ctcgggcctg ccggcgcgcg ctagagatcc ggcccaacta tgccaaggcc cacaacaacc
     6361 tcggcacggc gctcgccacc cagcaccgcg ccgaagacgc cgtcgcttgc tttcgccagg
     6421 cgattgcggt caacgccaac ttcgccgacg cctatcgcgg cctcggcgag gccttgttgg
     6481 cgctgcggcg cgaggacgaa gccgtggccg cgctcgagcg cgccgtcgat ctcagacccg
     6541 atgaggccga cgcacacgtc agcctcggca gcgcgctgag tgcccgccgg caaatgggcc
     6601 gcgccgccgc tgcctatcgg cgcgcccttg agctcaagcc cgagaacggc gtcgcccacc
     6661 acaacctggg caacgcgctc gccgccgccg gagacctcga tgccgccgcg gccagctacc
     6721 gcctcgccgt cgaacacatg ggcgaggact gtcacgcgct tttgcagtat gtgcaggtct
     6781 gccaccaaat gtgcgcctgg cagcaagcgg cgccgcaggc gcaagcgctc cttgagcggg
     6841 taggtcggcc ctccttcaat gctccgccct ttcccctcct tgccaccgtc gatgacccag
     6901 tgctgcagca taaggcggcg cgcgcctacg tcagccacgc catcggcagc ggctatcccg
     6961 cgttgtggaa gggcgagaga tatggccacc cgcgcctcaa actcgcatac ttctccttcg
     7021 acctgcgcga acatcccgcc gcgcgcttgg ttgtcgacct gatcgaacgc caccaccgtg
     7081 ggcaattcga ggtgtttgcc atctcctcgg gccccgacga caagagcgcc ctgcgccgca
     7141 gggtgtcgca ggcctgtgat cacttcatcg atgtcagcca gctcgctgat gcggcgatcg
     7201 ccgagaagct gcgcgccttg gagatcgacg tcgtcgtcga cctcgacggc cacaccgagg
     7261 gtagccgcag ccgcgcgctg gcgtggcggc cggtgccggc gcaggtcgcc tatctcggct
     7321 accccgcgac tatgggtgcc gatttcatcg actacgccat cgtcgaccgc tgcatcgtcc
     7381 ccgatgacca gcacccccgc ttcagcgaaa aattggtcta tttgcccgat tgcttccagg
     7441 tgagcgacag caagcggccg ttgccggcgc aggcagaacc gcgagccaag ctcggcctgc
     7501 ccgaggacgc gtttgtgttc gcctgcttca acaataccta caagatcacg ccggccgtgt
     7561 tcgaggtttg gatgcgcttg ctcggcgccg tgccgcacag cgtgctttgg ctggtggccg
     7621 acaatcgctg ggcggacgcc aatctcaaag aggcagcgcg ccaaaaccag atcgatccgg
     7681 cgcgcctgat ctttgccccg cgcgtcgagt acgcgagctt tctcaagcgc cagctcgccg
     7741 ccgatctctt tctcgatacg gccccctaca atggcggggc cacctgcaac gacgcgctct
     7801 gggccggcct acccgtcgtc acctgttgcg gccgcgccta cgcggcccgc atggcggcaa
     7861 gcctgctgca ggggatcggc atgcccgaac tcgtcaccac gtcgctcgag cattacgagg
     7921 ccttggcgct ggcgctggcc cgcgatcaag cagagctgac gcgcctcagg cgcaagcttg
     7981 tcgaggcgag gccgcagagc gcgctattcg ataccgagcg cttttgccgc aacctggaag
     8041 cggccttcgc cgaaatgcgc cgcgtcagcg aagccggcga gcaaccctcc tcgttttcga
     8101 tcgagaccgc agcttcgcca tgaggcgcgc tccggcaatc gaccgtcatt gcggcgcgct
     8161 atgaggctcg gcctagggtg agcagccgga ggtgctgtta tgtcgattga gatgcgcggc
     8221 gccgatatcg tcgtgcgcag cctcgaacgg gcgggctgca ccaacgtttt cacgttgtcc
     8281 ggcaatcaca tcatgccgct gtttgatgcc gtgctcggct cgaagctacg cttgttgcac
     8341 gtgcggcacg aagcggcggc cgtgcacatg gccgatgcgt ggggtcggct caccggtcaa
     8401 tgtggcatcg cgctggtgag cggcggggcc ggtcatgcca acgcgttggg cgcgctggcg
     8461 acggcggcag cggcagactc tcccctggtc ctgttgtcgg gccatgcggg actgaacgag
     8521 gtcggacgcg gcgccttcca ggaaatgcgc caggccgaca tggcagcccc ggtgaccaaa
     8581 gccgcctggg tggcacagca accggcggag ctggggcacg atatcgcccg cgccatgcgc
     8641 acggccttgt ctggccggcc cgggcccgta cacgtgagcc tgcccttcga tctgctgcaa
     8701 gcccgcgtgc cgccgagccc cgatcttgtg cctggccgcg aggccttcgg cgccgagccg
     8761 atgccctttg ccgccgccgc cgccgaggcc acgctgcggc acctctttgc ggcgcgccgc
     8821 cccttgatcc tcggcggtcc gcggctgtgt cagcgcgatg ggcccgccct gttgggcgag
     8881 ctcggcgccg ccacgggggt gccgagcttt ggcatggaaa gcccgcgtgg gcttgccgat
     8941 ccgcgccttg gcgcccttgc gcaggcgctc ggccgcgccg atctcatcgt gctcttgggc
     9001 aaggccctcg atttcacgct gcgcttcggg gatcagcccc acgttgccgc tgactgccgg
     9061 tttatcggca ttgatcccga ccccggtcag cttgcccggc tgagccgcag catgggcgat
     9121 cgggtgctgg tgcaggtcag cgccgaccct gtagccgcgg ccgcgtctct gcgcacgcgt
     9181 tgcggcccgc gcggcaccaa gaacgcctgg gccgaggagg tgacgtgggc gctcagtgtt
     9241 cgaccgcagg catcgacctt tgccgcggct ggccccggca agctccaccc catcgagctc
     9301 tgccgggcac tggcgcccta tctggaacga aatcgggatg cgatcttgat ctgcgacggc
     9361 ggcgagatcg gccaatggcc gcaagccgcc ctgtccccgc cccgacgcct aatcaacggc
     9421 gtggccggtg ccatcggagc cgccgttccc tttgccttgg ccgcgcgcct cgccgagccc
     9481 aaggcgccgg tgctggccgt catgggcgac ggcagcttcg gcttccatat ggcggaattc
     9541 gacaccgccg tgcgctatga cctgcccttc atcgccgttg ttggcaacga tgccacttgg
     9601 aatgccgaac accaaattca gctcagggac tatggcgccg aacgcacgca cgcctgcggc
     9661 ttgctgccaa gccgttatga cctggtggcg ctcgcgctcg gcggccacgg tgaactcgtc
     9721 acggccgccg atgcgctgcc cgcggcgctt gagcgcgcgc tggcggccgg taaaccggcc
     9781 tgcctcaatg tcatgatcga acgggtgccg gcgccgacct tgccagcgcg agcgccgctg
     9841 caagcgcccg acgccggcgc ctcggataag gttacctctt gagccggatg ggccaccgct
     9901 tatatctgtt ctattggggg ccatgccgct aagaggccat gccatgcgag catcgatcat
     9961 actcggcgcc gggatcctcc tggcggtgac ttttgccgcc caggcgcagt cctcccggca
    10021 gcagccgcgg caaccgccgc agaccccgcc agcggtcatc atcgagcgcg accgtctaca
    10081 accaccccct gcacccgaac gacgggtcgt cccgcccagg accttccagg acatttcccc
    10141 cccgatgcag gggccgacgc cgctgtcgcc catggccccg cgcatcggca actagcgccg
    10201 gcgcagctga ggcgtgccca cctcaggcgt gaatagcgcg tttgtccacg gcgagcgcgg
    10261 cctccttgac cgcctcggtc agggtcggat gggcatgaca ggtccgcgcc aagtcctccg
    10321 ctgagccgcc gaactcgagc atggccgtgg cctcggcgat catctcgctc gccgcggctg
    10381 caataatatg cacaccgaga atgcggtcgc taccggcatc ggccaagatc ttcacgaagc
    10441 cgtccgtggt tttgttgacc ttggcgcggg cgttcgccgt aaagggatat ttgcccacct
    10501 tgtaggcgac gttggccgcc ttcagctctt cctcgctttt gccgacgctc gccacctccg
    10561 gaaaggtgta gatcacattc ggaatggcgt catagttcac gtggccggcc tgcccagcca
    10621 gcgtctcggc gacggcaatg ccttcctctt cggctttgtg tgccagcatg ggacctgcga
    10681 ttacgtcgcc gatggcatag acgcctgcca cgttggtctg gtagcgcgca tccacaacaa
    10741 tgcgtttctt gttgtccagc tgcacgccga ccgcctccag cccgaggccg tcggtgtagg
    10801 gcacacggcc gaccgcgatc agcaccacgt ccgcctccac gccgatcgga gcccctcccg
    10861 ctgccggctc gatggtcacc ttttgctgag cgccgttctt ggcaatgcgc gtcaccttgc
    10921 tcgaaagctt gaaactcatg ccctgtttca tcaacaggcg ctgcaggccg cgcgccacct
    10981 cggcgtcggt gccgggcaga atccggtcaa gaaactcaac ggtcagcacc tcgctaccaa
    11041 gccgacgcca aaccgagccg agttccaagc cgatcacgcc ggcgccgata atcaacaagc
    11101 gcttgggcac ctgcggcagc gtgagggcac ccgtcgaaga caccacgctc gcctcatcga
    11161 tctcgacgcc gggcagccgg gccacatcgg accccgtggc gatcacgatg ctgcgcgctt
    11221 ccagctgctc gggggcggcg ccgtcgcgtg ccaccttcac ccgacccggc gccacaagcg
    11281 tgccaaggcc gtggaagatg tcgatcttgt tcttcttcag cagaaaggcg acgccatcga
    11341 cgttgccctt cacgccctgg tctttgaagg ccagcatgct cgcgagattg agcttgggca
    11401 ccacctcgat gcccatgccc gccaagtgat ggcgagcctc ctcgtaacgc tcggaggcgt
    11461 gcaacaaggc cttggacggg atgcagccga tattgagaca ggtaccgccg tgggtggcgc
    11521 gcttctcgat tacggccacc ttcaggccaa gctgtgcggc gcggatggcg caaacgtagc
    11581 cgccgggtcc cgtgccgatc acgaccagat cgtagctccc cgccatcgct caaagctcca
    11641 gcacgaggcg ctgcgggtcc tccaggaact ccttgacgcg caccaagaag gtgaccgctt
    11701 cacggccatc gaccagccgg tggtcgtaac tgagggccag gttcatgatc ggacgcgcga
    11761 cgatctgacc attgcgcacg acggcccgct cctcgatgcg atgcatgcca aggatgccgg
    11821 attggggcgc attaaggatc ggcgtcgaca gcagtgagcc atagacgcca ccgttcgaga
    11881 tcgtgaacgt gccgccctgc atttcttcga tgccgagctt accttcacgc gcgcggcggc
    11941 cgaactcgcc gatggcctgc tcgatttcgg cgaaggacag gcggtcggcc tcgcgcagca
    12001 ccggcaccac caggccacga tcggtgccga cggcgacgcc gatgtggtag tagttcttgt
    12061 agataatttc gtcaccatcg atctcggcgt tgacggcggg gacctccttc agggcctgca
    12121 cgcaggcctt cacgaaaaag gccatgaagc ccagtttggt gcggtagcgc ttttcgaaca
    12181 gctccttgta ggcgcggcgc agctcggtga tcgcgctcat gtccacgtca ttaaaggtcg
    12241 tgagcatggc cgccgtgttc tgcgcctcct tcagccggcg cgcgatggtc tggcgcagcc
    12301 gcgtcatgcg cacgcgctcc tcgcgcgtgg catcgttggg ccctgagggt gcgcgcagtg
    12361 cctgggtcgc aggcggcacg ctggcgggca gatagctccc cgccgcactg gtcgtagccg
    12421 gccccgcggc ccgccgcgcc agcgcggcct gggcatcttc cttgaggatc tgaccgcgcc
    12481 ggccggttcc cgccacctcg gcggcgctga ggtcctgttc ggccatgatt ttgcgcgctg
    12541 ccggggccgg tggcatggcc ccatcgctcg ccggctcgtc ggcaggggcg ggccgttccg
    12601 ccgccctcgg cgcaggcgcg gccggctgcg gctt
//
LOCUS       DASTMT010000212         3163 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_1749425, whole genome shotgun sequence.
ACCESSION   DASTMT010000212 DASTMT010000000
VERSION     DASTMT010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3163
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1749425"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..668)
                     /locus_tag="VFR00_04985"
     CDS             complement(<1..668)
                     /locus_tag="VFR00_04985"
                     /inference="COORDINATES: protein motif:HMM:NF024882.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HEU0158641.1"
                     /translation="MAYQRLDHTPLLPASLRHRLGSWLMRATGSGVLVACAACGLALA
                     TWSAADPSLSHVTTGTIRNLLGSPGAILADLVMQLLGLAGVLILLPPLLWAVPLSSGR
                     ALPAWRGKVALAPIAVVAIAGALSALPTSLSWSLHHGNGGMIGDLTFTLLASAFAPFG
                     AAKAALTASLLLGAGGGLALMGSLGLSREEWRQILAYPPAPRLGAVAAAWRTLPRWLP
                     QPTR"
     gene            complement(685..1905)
                     /locus_tag="VFR00_04990"
     CDS             complement(685..1905)
                     /locus_tag="VFR00_04990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013950205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEU0158642.1"
                     /translation="MHAATGLETLAQGAVLSPFTRINRLLAGLAPGHARVIEMTAGDP
                     KEVMPGFVPDKLVEAKALLSTYPKIRGSDDLRAAIASWLGRRYGIADKIDPAREIHPL
                     NGSREGLFFAALPAVGRKSFSTRPLMLLPNPFYQAYLGATLGTNCEPFYLNATAETGH
                     LPDLDALAQEPEVLHRVAAFYLCSPANPQGAVASAAFLRKALALARQYDFMLFLDECY
                     SEIYAGENAPTGALQIAAATPERYKNLIVFNSLSKRSNLPGLRSGFAAGDGDFLETLA
                     EIRNLTAPQMPGIVQHASTAVWSEEQHVTVIRQAYRVKFDICDEVLAGRFGYRRPAGG
                     FFLWLDMSHLGGAEQATLTLWKRCGVKVTPGVYLAHEDRHGVNPGRDYIRVALVHNAA
                     TIREALERIVAVSA"
     gene            complement(2006..>3163)
                     /locus_tag="VFR00_04995"
     CDS             complement(2006..>3163)
                     /locus_tag="VFR00_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEU0158643.1"
                     /translation="GFAFLEAGTVRHKNQVNALCKILVDFSISTVAYFFVGFAIAYGT
                     TFLVSAEVLSGGGKGFDPQGYTLVKFFFLVTFAAATPAIVSGGIAERARFLPQCVATG
                     VIVGLFYPFFEGIVWNKNFGLQEVFFKNLLGEEFHDFAGSIVVHAVGGWIGFSAVLIL
                     GPRIGRYDSKRTSGIPPSSIPWLAMGSWLLCIGWFGFNVMSAQSLKTVTGLVAMNSLM
                     AMVGGIIAALLAGDGDPGFIHNGALAGLVAVCAGSDVMHPVGSLIVGGVAGVIFVYAF
                     QIATSSKYKLDDVLGVWPLHGLCGLWGGIACGIFGLKALGGMGGVSFSSQLVGSVLGA
                     AFGAFAGYVIYTAVDMLLGLRLTSEEERRGADLAVHKIGANPEEDVRMGRI"
BASE COUNT          664 a          991 c         1067 g          441 t
ORIGIN      
        1 atgcgggtgg gctgcggcag ccaacgcggc aaggtccgcc acgccgccgc caccgcaccg
       61 agccgcggcg cgggcggata ggccaggatc tggcgccatt cctcgcgcga gagcccgagg
      121 ctgcccatga gcgccaagcc gccccccgcc cccagcagca ggctggcggt gagcgcggcc
      181 ttggcggcgc cgaagggtgc aaaagcgctc gccagcaacg tgaaggtgag atcgccgatc
      241 atgccgccgt tgccgtggtg caacgaccag ctgagcgagg ttggaagcgc cgacagggca
      301 ccggcgattg ccaccacggc aatgggtgcc aaggccacct tgccgcgcca tgccggcaac
      361 gcccgcccgc tgctcaaggg cacagcccat agcagcggcg gcaagaggat gaggacgccg
      421 gcaagcccaa gcagctgcat caccaggtcg gcaagaatgg cgcccggtga tcccagcagg
      481 ttgcggatgg tgccggtggt gacgtgggag agactggggt cggcagccga ccaagtcgcg
      541 agcgcgagac cgcaggccgc acaggcgacg agcacgccgg accccgtggc gcgcatgagc
      601 catgacccca agcgatggcg cagggacgcc ggcagcagcg gggtatgatc gagacgctgg
      661 tacgccatgc caccacaaac aaggttacgc tgagaccgca acgatccgct cgagcgcttc
      721 cctgatcgtc gccgcgttgt gcaccagcgc aacgcggatg tagtcgcggc cgggattgac
      781 gccgtgccgg tcctcgtgcg ccaggtagac gcccggagta accttgacac cgcagcgttt
      841 ccagagggtt aaggtggctt gctcggcgcc acccaaatgg ctcatatcga gccaaagaaa
      901 gaacccaccc gccggccgcc ggtagccgaa gcgcccggcc agcacctcat cacaaatatc
      961 gaatttgacg cggtaggcct ggcgaatgac cgtgacgtgc tgctcctccg accaaacggc
     1021 cgtcgaggca tgctggacga tgcctggcat ttgcggcgcc gtcaggttgc ggatctcggc
     1081 cagcgtttcc aggaaatcgc catcgcccgc cgcaaagccc gagcgcaggc cgggcaaatt
     1141 cgagcgcttg gacagcgaat tgaagacaat gagatttttg tagcgctcag gggtggcggc
     1201 ggcaatctgc agcgcacccg tgggcgcgtt ctcgccggcg tagatttccg aatagcactc
     1261 atcgaggaac agcatgaagt cgtactggcg cgcgagcgcc aaggccttgc gcaaaaaggc
     1321 agccgatgca accgcccctt gcggattggc tggggagcag aggtagaagg cggccacccg
     1381 gtgcagaacc tcgggctctt gtgccagggc gtcgaggtcg ggcaagtgcc ccgtctcggc
     1441 cgtggcattg aggtagaacg gctcgcagtt ggtcccgagc gtggccccga gataggcttg
     1501 atagaacggg ttgggcaaca gcatcagcgg gcgagtggag aaggacttgc ggccgacggc
     1561 gggcagggcg gcaaaaaaga gaccctcgcg cgaaccgttg agaggatgga tctcgcgcgc
     1621 tggatcgatc ttgtcggcaa tgccgtagcg gcgcccaagc cagctcgcta tcgctgcgcg
     1681 caggtcatcc gagccgcgga tcttgggata ggtcgagagc agggctttgg cctcgacgag
     1741 cttatcggga acgaagccgg gcatcacctc cttggggtcg ccggccgtca tctcgatgac
     1801 gcgggcgtga ccaggtgcaa gaccggcgag caggcgattg atgcgcgtga acggcgacag
     1861 aacggcgccc tgggcgaggg tttcaaggcc ggtggcagcg tgcatgggtg ttccgctcga
     1921 aaaggaaggc aagctggcgc gagcttaaaa cgcccgcgcg ccgatcggga agccataaca
     1981 ggggcgaggt ggccggcgcg gcgccctaga tgcggcccat gcgcacatct tcctccggat
     2041 tggcgccaat cttgtgcacg gcgaggtcgg cgccgcggcg ctcctcctcc gatgtcagcc
     2101 gcagccccaa gagcatgtcg acggcggtgt agatgacgta gccggcgaac gcgccgaagg
     2161 cggcgcccag gacgctgccg acgagctgcg aggagaagct gacccctccc ataccgccca
     2221 gggccttgag gccaaaaatg ccgcaggcaa tcccgcccca cagtccacac aacccgtgca
     2281 ggggccacac ccccagcacg tcatcgagct tgtatttgct gctggtggcg atctgaaagg
     2341 cataaacgaa gatcacgcca gcaaccccgc cgacgatcag cgaacccacg gggtgcatga
     2401 cgtcggagcc cgcgcacacc gcaacgagac ccgccagcgc gccgttatgg ataaagcctg
     2461 gatcaccgtc accggcgagc agcgccgcga tgatgccgcc taccatggcc atcagcgagt
     2521 tcatggcgac gagcccggtc acggttttta aggactgcgc gctcatcacg ttgaagccga
     2581 accagccgat acagaggagc caagagccca tggctaacca ggggatggag gagggcggaa
     2641 tgccgctggt gcgcttgcta tcgtagcggc caatgcgcgg ccccaggatg agtacggccg
     2701 agaacccgat ccagccgccg acggcgtgca ccacgatcga accggcgaag tcatggaatt
     2761 cctcgcccag caggtttttg aaaaaaacct cctgtaggcc aaagttcttg ttccagacaa
     2821 tgccctcgaa gaaggggtag aacagaccga cgataacgcc ggtggcaacg cattggggca
     2881 ggaagcgcgc gcgctcggca atgccgcccg aaacgatggc tggcgtggcg gcggcgaacg
     2941 tcaccaggaa gaagaatttg accagcgtgt aaccctgggg atcgaagccc ttgccgcccc
     3001 cgctcagaac ctcggcactg acaagaaagg ttgtgccata agcgatggcg aagccgacga
     3061 agaaataggc aaccgtggag atcgaaaagt cgaccaagat cttgcagagc gcattgacct
     3121 ggttcttgtg acgaacggtg cctgcctcca ggaaggcgaa gcc
//
LOCUS       DASTMT010000213         2648 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_2734273, whole genome shotgun sequence.
ACCESSION   DASTMT010000213 DASTMT010000000
VERSION     DASTMT010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2648
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2734273"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..642)
                     /locus_tag="VFR00_05000"
     CDS             complement(<1..642)
                     /locus_tag="VFR00_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEU0158644.1"
                     /translation="MQTTTFAELGLSAKVQAAIQAAGYSIPTPIQQQAIPVAVSGRDV
                     LGIAQTGTGKTAGFVLPMLARLETGRARARMPRSLILAPTRELAAQVAQSFEKYGVNH
                     KLNLALLIGGVSFDEQLRKLDRGVDVLIATPGRLLDHFGRGKILLTGVEILVIDEADR
                     MLDMGFIPDIERICKLLPPARQTLFFSATMPPEIQRLVNTFLKDPARVEVAPPA"
     gene            1054..1761
                     /locus_tag="VFR00_05005"
     CDS             1054..1761
                     /locus_tag="VFR00_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011443017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEU0158645.1"
                     /translation="MPSKINLVLIPGLLCSPALWAAQIRALNDIADATVADHTRHDSM
                     HEIAAAILAAAPPRFALAGLSMGGYIAYEIIRQAAERVTHVALLDTGARADAPERREG
                     RLRLIALAEQHGAVRAQQELLPLLVHTDRLGDAALVDTVLQMAVDTGLAAFKRQQTAI
                     MARPDNRALLSSIRCPSLVLVGREDALTPPALAQEIADGILGSRLEIIPNCGHLSSLE
                     RPEAVSRALRAWLGATP"
     gene            complement(1895..2566)
                     /locus_tag="VFR00_05010"
     CDS             complement(1895..2566)
                     /locus_tag="VFR00_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HEU0158646.1"
                     /translation="MPQRALAAEFIGTFMLMSSVLGAAFYSFAAPSGAAGILGVALSI
                     GATVMALAFAIGHISGGHFNPAVTFGLIAGGRFKTSSALGYVVAQCLGALGACGLFSL
                     IGKAPPTFAANGYGSLSMLQASLFSVFLIELVLTAFLVFIIMGVTNRRAPAGFAPLAI
                     GLTLTAIHLMAIPISNASVNPARSLGSAVFGGTTALSQLWLFWVAPIIGGVIGGALAR
                     WLQEE"
BASE COUNT          502 a          848 c          868 g          430 t
ORIGIN      
        1 cgccggcggc gccacctcca cccgcgccgg atccttcagg aaggtattca ccaggcgctg
       61 gatctcgggc ggcatggtgg ccgagaaaaa caacgtctgt cgcgccggcg gcaacagttt
      121 gcagatgcgc tcgatatccg ggatgaaacc catgtcgagc atgcggtcgg cctcgtcgat
      181 caccaggatc tcgacgccgg tgagcagaat tttgccgcga ccaaaatgat cgagcagacg
      241 acccggcgtg gcgatcagca catcgacgcc acgatcgagt ttgcgcaatt gctcatcgaa
      301 ggaaacgccg ccgatcagca gcgccaggtt gagcttgtgg ttgaccccgt atttctcgaa
      361 gctctgggcc acctgggcgg caagctcgcg ggtcggggca aggatcaagg agcggggcat
      421 gcgcgcccgc gcccgaccgg tttccagccg cgccaacatg ggcagcacga agccggcggt
      481 cttgccggtg cccgtctggg caatgccgag gacatcgcgt cccgatacgg cgaccggaat
      541 ggcctgttgt tgaatggggg tgggaatgct gtagccggca gcctggatgg ctgcctgtac
      601 cttcgcgctg aggccaagct cagcgaacgt cgttgtctgc aaaagaacga atctccgtct
      661 gcctaccgag ccaatgctcg cgcaacgcga gtgggcgcaa gcaccagcaa ccagagcgca
      721 gggggattag ccgcaaaagg gcctaacggg aaagcatctg cttggggaaa atggattatg
      781 aaagcgatcc cctgcgcccg ttacacgctc gtagatggca ctgttcgcct gaaaaatcaa
      841 gttcatcagg atcacgcttc tgtgggtcag atcgcctcgg cggtcgggcc gttctcgccc
      901 cgaggctttg tctcgtccgc ttggcaccgc caaggtggcg cttttgcggt ttaccctttg
      961 ggagagtgcc cccggcaagc gcgcgtgcca cactcattgg ccagctcgaa cgctgccaat
     1021 ggtgtggcgc cgcaggcgca cttggagagg acaatgccgt caaaaatcaa cctcgtgctt
     1081 atcccgggcc tgctctgctc gccggcgttg tgggcggcac aaatccgtgc tctgaacgat
     1141 attgccgacg ccacggtcgc cgatcacacc cgtcatgaca gcatgcacga gatcgccgcg
     1201 gccattctcg ctgctgcacc gccgcgcttt gcactcgcgg gcctgtcgat ggggggatac
     1261 atcgcctatg agatcatccg ccaggccgcc gagcgcgtga cgcacgtcgc gcttcttgac
     1321 accggcgcgc gcgccgatgc gccggaacgg cgtgagggac ggttgcgtct gatcgcgctg
     1381 gccgagcaac atggtgccgt cagggcgcag caggaattgt tgccccttct cgtgcacacg
     1441 gatcggctcg gcgatgcggc gctggtcgac acggtgctgc aaatggccgt cgatacgggg
     1501 cttgccgcct tcaagcggca acaaacggcg atcatggctc gcccggacaa ccgagcgcta
     1561 ttgtcgagca tcaggtgccc gagccttgtc ttggtcgggc gcgaggacgc cctgacgccg
     1621 ccggcgctgg cacaggagat cgccgatggc attctcggct cgcggctcga gatcatcccc
     1681 aattgtgggc acctctccag cttggagcga ccggaggcgg tctcgcgcgc cttgcgcgcc
     1741 tggctcggtg ccacgcctta gatcggcggc aggcgcggtc cgacgcgcag cggtgacgcc
     1801 ggcgttgcgc gcacgggcgc tcgcgcctcc gccggcggcg ctggcaaccg cgccacggct
     1861 ttttctggct ctgccctgcc tccatctttg cgggctattc ctcctgcagc cagcgtgcca
     1921 gcgcaccgcc gatcaccccg ccgatgatcg gcgccaccca gaacagccaa agctggctca
     1981 aggcggtggt gccgccgaac acggcgctgc cgaggctacg cgccggattg accgaggcat
     2041 tggagatggg gatcgccatc aaatgaatgg ccgtcagggt gagaccgatg gccaacggcg
     2101 cgaagcccgc gggcgcgcgc cggttggtga cgcccatgat gatgaagacc aggaaggcgg
     2161 tcagcaccag ttcgatcagg aataccgaga aaaggctcgc ttgcagcatc gagagcgaac
     2221 catagccatt ggcggcaaag gttggaggcg ccttgccgat cagactgaag aggccgcagg
     2281 caccaagcgc gcccagacac tgggccacca cgtagcccag ggcgctgctg gttttgaagc
     2341 gcccgccggc gatcagcccg aaggtgacgg cgggattgaa atggcccccg gaaatgtggc
     2401 cgatggcaaa ggcaagcgcc ataacggtcg cgccgatgga tagggcgacg ccaagaatac
     2461 ccgccgcccc cgacggggca gcgaaggaat aaaaggcagc ccccagcacc gaggacatca
     2521 acataaaagt gccgataaat tcggcggcca gcgcacgttg tggcatgggc atccccctaa
     2581 aaagtctccc ctggcgccga atcgcggcga cggcggcgat cttagcaaag cggggcgccc
     2641 ctttgcgc
//
LOCUS       DASTMT010000214         3801 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_4323574, whole genome shotgun sequence.
ACCESSION   DASTMT010000214 DASTMT010000000
VERSION     DASTMT010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3801
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4323574"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..312
                     /locus_tag="VFR00_05015"
     CDS             <1..312
                     /locus_tag="VFR00_05015"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEU0158647.1"
                     /translation="AKEIFYSAGKFSAEDARIMGLVNRVVPPAELESYVAKFASTISA
                     NAPLTIKAAKMAINAAVEDAERRRMSEIDATIKACFASEDYIEGRRAFMEKRKPAFKG
                     R"
     gene            complement(377..1333)
                     /locus_tag="VFR00_05020"
     CDS             complement(377..1333)
                     /locus_tag="VFR00_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003610932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HEU0158648.1"
                     /translation="MRHMDSLTEQELLALAISLEEEHARIYADYAEGLRQDYPASAQL
                     FQEMAEEENEHRRQLIELYREKFGEHIPLVRRHDVKGFLRHEPLWIVRPLGLDKVRKQ
                     AEVIELETRRFYERALMRSTDAGIRKLLGDLAAAEREHIDLAQKLSQELLTGGAKATE
                     EAARQRAFVLQVVQPGLAGLMDGSVSTLAPLFAAAFATHNTWETFLVGLAASVGAGIS
                     MGFAEALSDDGELTGRGPPLLRGAITGAMTALGGIGHTLPYLIGDFAVATSIAICVVL
                     VELWVIAWVRSRYMDTKFLRAAFEVVVGGLIVFAAGILIGSA"
     gene            1537..2469
                     /locus_tag="VFR00_05025"
                     /pseudo
     CDS             1537..2469
                     /locus_tag="VFR00_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015688824.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            complement(2539..2775)
                     /locus_tag="VFR00_05030"
     CDS             complement(2539..2775)
                     /locus_tag="VFR00_05030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0158649.1"
                     /translation="MPIDWTTIPVMPLGLLAALTFVASLIGNSLSRSAFVGSILTVII
                     FVAFYVFWNHYDTPLQAMAGNIHFPNNWVPMRGR"
     gene            3209..>3801
                     /locus_tag="VFR00_05035"
     CDS             3209..>3801
                     /locus_tag="VFR00_05035"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEU0158650.1"
                     /translation="MDAFVFMAVLAAAACHAGWNALLKLNVAPVVATVLVAVAAGLLV
                     LPLLPITPLPAAAAWPYIVASALIHVAYFVALAEAYRWGDLSQVYPIARGTAPLLTAA
                     LASLWLNEALGSNGWAGVIVLAAGILLLTVKGARKLAPFDHRAVAFALATSLTITAYT
                     LVDGIGARLAGSALAYTVWLFIGDGITMAIYGALRVGP"
BASE COUNT          671 a         1234 c         1231 g          665 t
ORIGIN      
        1 gccaaggaga tcttttactc ggcgggcaag ttcagcgccg aggatgcacg catcatgggg
       61 ctggtgaacc gggtggtgcc gcccgccgag cttgagagct atgtggcgaa gtttgcctcc
      121 accatcagcg ccaacgcgcc gctcaccatc aaggcggcca agatggcgat caacgcggcc
      181 gtcgaagacg cggagcggcg gcgtatgagc gaaatcgacg ccaccatcaa agcctgcttt
      241 gccagcgagg attatatcga gggccgccgc gccttcatgg aaaagcgcaa acccgccttc
      301 aaggggcgct gacgccgcgt ccgccgcggc gccggccggt gcgatcgcgt ggagctttgg
      361 ctctcgaacg tgcggctcag gcgctgccga ttaggatgcc agcggcgaac acgatcaggc
      421 ctccgaccac gacctcgaag gcggcccgca ggaatttcgt gtccatgtag cgcgaccgca
      481 cccaggcgat cacccagagt tcgaccagca ccacgcaaat ggcgatgctg gtggccacgg
      541 caaaatcccc aatgagatag ggcaaggtgt ggccgatgcc gcccagcgcc gtcattgccc
      601 cggtaatggc acccctgagc agcggtggac cgcggccggt cagctcgcca tcatcggaga
      661 gggcttcggc aaagcccatg gaaatgcccg ccccgaccga ggcggcaagg ccgacgagaa
      721 aggtttccca ggtgttgtgg gtggcgaagg cggcggcaaa cagcggcgcc agcgtcgaga
      781 ccgagccatc catcaagccg gcgagcccgg gttgcaccac ctgcaaaaca aaggcgcgct
      841 ggcgcgcggc ctcctcggtg gctttggctc cgcccgtcaa cagctcctgg gagagtttct
      901 gggcgaggtc gatatgctcg cgctcggcgg cggccagatc gcccaacagc ttgcggatgc
      961 ccgcgtcggt gctgcgcatc agcgcgcgct catagaaacg gcgggtctcc aattcgatca
     1021 cctcggcctg cttgcgcacc ttgtcgaggc caagcggtcg cacgatccaa agcggctcat
     1081 gcctgaggaa gcccttgacg tcgtgtcggc gcacgagcgg aatgtgttcg ccgaatttct
     1141 cgcggtagag ctcgatcagc tgccgccggt gctcgttttc ctcttcggcc atctcctgaa
     1201 acagctgggc ggatgccgga tagtcttggc gtagcccctc ggcatagtcg gcatagatgc
     1261 gcgcgtgttc ctcctcgagc gaaatggcga gcgcgagcag ctcctgctcc gtcaggctgt
     1321 ccatgtgccg catggcaaca cccatccttc gagcctttga ttttagaata attctaaata
     1381 agggcgcgcg gcaatgggcg atgatcgcga cggccggcag tccggccagg ctcggccagg
     1441 gtgccgggcc gcggcgcagc ggctcccgcg tgggctgcgt tttcagggcc gggcagatta
     1501 tggcaagcga gcacgacacc taaggcccgg aagcccctgc agcgcagaca attcctgaag
     1561 cgcgccggtg ttggtttggc gagcacggcc gtggcggtgc cggccatcgc tcagtccgcg
     1621 ccgaccatgc gctggcgact ggcaacgtcg cttccaaagt cgctcgatac gctctctggc
     1681 gcctgcgatt gttccgtaag taccttgccg aggtctccga tcggaagtct ctggcgtcgt
     1741 tgtttgtttc cgccattcag gtgttcgacg ccggactaga gctgcgcaac gacctctaca
     1801 agggccacaa cgtcaggggc gttcccttcg gcaatgccac cgtgcagatg ggcggttggt
     1861 tccgcaaagg catcaaaccg tcgatgacct gatggtctca aatgtcgcgt cggtggcgtt
     1921 gccggccagg tgctcgggcg gcgcggcgtc gttcctcagc agctgccgcc cgccgatatc
     1981 tatccagcgc tggagaaggg cacgatcgat gcggccgagt ggatcggacg ctatgacgat
     2041 gaaaagctcg gcttcgtgaa gatcgcgcct tacgacgact atcccggctg atgggaaggc
     2101 tgcgccaacg gctttctcta tatcaacagc gccaagtggg ccgagctgcc caagacctat
     2161 cagcagatgg tcaccacgat cgccgggcac gtggcggccg atctcattgc caaatacgac
     2221 agccgcaacc cacccgccat caaaggcttg gtggcggtcg gcgcgcaact gcgcccgttc
     2281 tcgcttgata tatgggatgc ctgctacaag gcgagccaag acctgttcgc cgaggtgtcg
     2341 gccaagaacg cggtcttcaa gacctgctat gaatccatgc tgggcttccg caacgacccg
     2401 tacgcctggc atcagatctg cgaggtgaca tacgacgcct atgtggtccg gcaacggagc
     2461 cggacctgag cgtccacacc agcgctcaag gaatactcgg cgctcttctg gccgtgcggc
     2521 caccgagcgc cgagcacatc agcgacctcg catcggcacc cagttgttcg ggaaatggat
     2581 gttgcccgcc atcgcctgca gcggcgtgtc gtagtgattc cagaagacgt agaaggcaac
     2641 gaaaataatc acggtcagga tcgaaccgac gaaggcgctt cggctcaaag agttgccgat
     2701 cagcgaggcc acaaaggtca gtgcggccag aagcccgagg ggcatgacgg ggatggtcgt
     2761 ccagtcgatt ggcataacga tgctcctgtt ggctctccgc accgatgtgg tccccacggg
     2821 accagatcag gcttaaactt cgaggtggcg tcaagcggaa caccgccgcg cgcgaagatg
     2881 caatgcctgc cgattgtggc gcggcgaaag caaaagccgg gcccggcacg cccaccccca
     2941 agcgggtcga acccggccca cggcggggcc gatccggccg cgccagcgcc gcggggccag
     3001 caagggtttg ccaacgctgg cgaaattgca cccacgcggc cgcggcaagg ggggcgcttc
     3061 cgcgccccgg cggcaaaacc gagccggggg cgcccccctt atgttgcaag tttgcggcga
     3121 gctcagtagc gtcaactttc tcgtgcttcc ggccttttgc ggcggccgac aaggccgctc
     3181 gggcccctct aaccaggcgc atgcgcagtt ggatgccttc gttttcatgg ccgtgctcgc
     3241 cgcggcggct tgtcacgcgg gctggaatgc gctcctcaag ttgaacgtcg cccccgtggt
     3301 cgccacggtc ttggttgccg tggccgccgg gctcctggtg ctgccgctgc tgcccattac
     3361 gccgctcccg gccgcggccg cctggcccta tatcgtcgcc tcggccctca ttcacgtcgc
     3421 ctatttcgtg gcgctggcgg aagcctaccg ctggggagat ctgagccaag tctatccgat
     3481 cgcccgcggc acggcgccgc tgttgaccgc ggcgcttgcc agcctgtggc tcaacgaagc
     3541 gctgggaagc aacgggtggg ccggcgtcat tgtgttggcg gccggcattc tgctcttgac
     3601 cgtcaagggc gctcgcaagc tcgcgccctt cgatcatcgt gcggttgcct tcgcgctcgc
     3661 cacctcgctg accatcaccg cctatacgct ggttgatggc atcggggcgc ggctggccgg
     3721 cagcgcgctc gcctatacgg tgtggctctt catcggcgat ggcatcacga tggcgatcta
     3781 cggggcgctg cgcgtcggtc c
//
LOCUS       DASTMT010000215        13751 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_769732, whole genome shotgun sequence.
ACCESSION   DASTMT010000215 DASTMT010000000
VERSION     DASTMT010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 13751)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13751)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13751
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_769732"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(11..910)
                     /locus_tag="VFR00_05040"
     CDS             complement(11..910)
                     /locus_tag="VFR00_05040"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU0158651.1"
                     /translation="MPKTLFIDSTPDIDRLWQRVHGPQDAPIDVNMGPVAEADIARLL
                     TGYDTTIVDATYFTAATLKRCRDLKHIVFLGTGAGSFIDLAAAATAGIKVSTIKGYGD
                     TAVAEHTMGLVFAAARNIASMHKIMREGGWQVMQGLELKGKTLGLIGLGGIGREMARL
                     GQGIGLEVIAFNRSPLPDAPVPMVSLEALLARADVVSLHLALNDGTRHFLDRGRLERL
                     KPGAILVNTARAGLLDEAALVELVRGGRIGHYATDVYAAEPPPASEPLRLLERVTLTA
                     HAAYNTPEAAMTMYRRAIALAAA"
     gene            1131..2426
                     /locus_tag="VFR00_05045"
     CDS             1131..2426
                     /locus_tag="VFR00_05045"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEU0158652.1"
                     /translation="MRGRILLKGAATAMLAATLWANPGSAQEREIKVGYMKNPIQDAS
                     LDMMEKWAKANNVKLTREPMAYSVFMEKVTATLTSGADQFDMIWHNDDWGQLWKKWVE
                     PTDDIKGLDTVDQWPLLAFWNDDKKLTVVPMAHTVGTFFYRSDLVKPEEVPKTFGELV
                     TVSQRLQKEGKVKWGYVGGMTMNNTWFSFWWTMWANQCDILKPLLERDNEKLKAAKFE
                     PAITEPCHREIVEYWWDAINTHKISPPGMTAYGRNEANAIFMAGDAAFTLVDSTHFGE
                     FSDPKRSKIVGKVSMAPFPVGPRAKVPTSWNEIWGWAIPIGVPAEKKKIVKDMLSAMM
                     SDEAGQIEMWKKTGGPPPNIKLWPKLAAEDKDFAALKHAVFDQKPITHSAYYFAEWPA
                     VHKAYSDAVIEAISGKREDIGKVLEAHVEKVRRAGLGTN"
     gene            2559..3458
                     /locus_tag="VFR00_05050"
     CDS             2559..3458
                     /locus_tag="VFR00_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020188259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU0158653.1"
                     /translation="MPQRSLPRIDEKKRFMLALIAPAVTVLIAFQILPILIGANASFR
                     DWHLHDPKRTWIGFTNYAYVLRDNEFLAIVLPNTFGFMLASVGTSLILGLAIALLLNR
                     KFAGRWLVQTIILLPLMVAPVIAAIMIRWMFNDQFGIVNVVLGVFGVPQIAWLTQKWT
                     AFLAILLTDVWLWTPWFALLLLAGLQSLPPEPFEAAQIDAASAWRTFRYITLPMLRPV
                     IVVCIVIRAIDAFRTFDIVWTISGGGPARATEVFSIYAYVEAFQFLNFGRGSAAAVIG
                     ALIIVVFAMALYRLLNRWVEVSR"
     gene            3455..4312
                     /locus_tag="VFR00_05055"
     CDS             3455..4312
                     /locus_tag="VFR00_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020188260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEU0158654.1"
                     /translation="MSAVEALPALASRNRFFDALAPGGRRALFVLGLIGVCLLFAFPV
                     FWLVLTSLRPGYAVYYVHRGTDFTLDNFHEVLSQEIVLRALWNSFAISTLATVLSLGV
                     TVTSGYMLSRFRGPIPNLWFSAIYVFRCVPYVSWVLPLYFVTRALGIFDTYWGLLLPH
                     VAVHICFFSWIMKGFFDGIDPSMEYAAMIDGCSRWGAFLRVALPAAIPGLTALAILCW
                     LYTWNEFLFALILTAQKTPILTVVMAQFVHELGMEWHLMSATAVLSLGPALIVTIFGQ
                     KYVIRGLKI"
     gene            4316..5416
                     /gene="ugpC"
                     /locus_tag="VFR00_05060"
     CDS             4316..5416
                     /gene="ugpC"
                     /locus_tag="VFR00_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020188261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HEU0158655.1"
                     /translation="MAEVTLSNVRKTYPGGVAAVKDFNLHIADHEFVVLVGPSGCGKS
                     TTLRMVAGLEDISGGTIAIGDRIVNGLQPKDRDIAMVFQNYALYQHMSVYDNLAFGLR
                     NRRAKEADIDAAVRGAAKILSIEPLLERRPRQLSGGQQQRVALGRCIVRNPKVFLFDE
                     PLSNLDAQLRAQMRLELKELRQRVPTTSVFVTHDQVEAMTLGDRIVVMKDGLVQQVGS
                     PLALFRQPINRFVASFIGSPAMNFLDMAVAAQADSFRLTAEGVAIELPQLRFPRLGAH
                     VGQSVTLGLRPQHLRLGSAGNGAQVGFSGNLMVTEQLGDEQLLAVRIGRGDLRVSGVD
                     PELALTTGTRIDLVVPIESLHIFDGSTGAAIR"
     gene            5455..6225
                     /locus_tag="VFR00_05065"
     CDS             5455..6225
                     /locus_tag="VFR00_05065"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU0158656.1"
                     /translation="MQGLAGRRALVTGAGNGIGRAIAFELARQGAALALVDIDGPAAE
                     ATAKELRSSGCAVAVAVADVSDAAAVKAAVSAFEASGEPLEILVNNAGIARLGSLLSI
                     SEQDWRDTFAVNVDGMFHATRAVVPGMVARRRGAVVNLASWLGRRGNALFGAYAASKF
                     AVIGLTQSLAAEVAPCGVRVNAVCPGIIADTPMRSALERAGAAAGLAPTEARVKAIPL
                     RRAGRPDEVAKVVAFLASDAAAYVTGAAYDVSGGHWMT"
     gene            6239..8023
                     /locus_tag="VFR00_05070"
     CDS             6239..8023
                     /locus_tag="VFR00_05070"
                     /inference="COORDINATES: protein motif:HMM:NF012927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0158657.1"
                     /translation="MTSLFEPGRIGPVQVPNRIVMAPMTTRAADREGFVTAQTLAYYG
                     ARARGGVGLITVEMASPERCGRHRQHELGIFDDRFLPGLARLASAIHAEGAKASIQLG
                     HAGGHTRPDICGEPPIAPSAIPHLVEEVTTETIIPEPMSKQRIEETTAHFVAAARRAG
                     AAGFDCVEIHAAHGYLISQFHTPFENRRSDEYGGSLQNRARFGLELTRRVKSAVAELG
                     VIYRLTVDDFFPQGLQLEEGLSIAEWAQRSGADALHIAAGHYRSLPGTPGMIPPMAWP
                     DAPFLKFAAALKPRVALPVIAVGRLGDPSTAAAALRRGACDFVALGRALLADAEWARK
                     VKTGAPVRACLACNTCISGMRGGGALNCLVNAETGREAAFAARVPLRGKRIAVIGAGP
                     AGLTYASLVGGSNEVVVFERDAWPGGSLRDAGRAPRFQGVAASRASLARYVASLEAAC
                     AHAGVRLRYGVNVAQRPEVLAGFDRLVVATGADYRLGLGRPLRALLRWGVARWPGVRG
                     LFARPWLLDFLYYRARRPSGAAIARLAPAGTEVVVIGDAAEPGKSARAIESAFAAAWD
                     GTLGAQRNQAAQPPSDAAKPAATEHETH"
     gene            8519..9418
                     /locus_tag="VFR00_05075"
     CDS             8519..9418
                     /locus_tag="VFR00_05075"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HEU0158658.1"
                     /translation="MRIALCNEVVRELSFERQCAFARAVGYDGLEIAPFTLGEEPHRL
                     APSRRSAIRAAAAAEGIAITSLHYLLLAPAGLSITAAAGAQHDRTIEVMRRLCDLAAD
                     LGAGVLVHGSPAQRQLEVGKEAEGRQRAIAAFAAAAEAAGSAGVTYCIEPLAQTETGF
                     VNTIAEAAAIVREVGHAALKTMLDCSAAARAEPQAIPELLRHWLPSGLIAHVHCNDPN
                     RRGPGEGSLAFAPILQALRTGAYRGMAAVEPFVYEPDGPACAARAIGYLRGLQQGAPA
                     QETPRPGPGDSLGEGVGGPQWAG"
     gene            complement(9517..9807)
                     /locus_tag="VFR00_05080"
     CDS             complement(9517..9807)
                     /locus_tag="VFR00_05080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0158659.1"
                     /translation="MKRQADTETTIIVLALLVGYLAYLRIVDWMPDLGLIGNLATTDV
                     SVLGGVEIKYHSILMLCALAICWAILLKVRRASLERAGRDDLARHGLSRHSL"
     gene            complement(10036..10284)
                     /locus_tag="VFR00_05085"
     CDS             complement(10036..10284)
                     /locus_tag="VFR00_05085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0158660.1"
                     /translation="MSQDTKIDLQQPIDEFLLALAEASLVDFTPEAIMILEDIVAEDR
                     PRHLEFAIAVYPSPRAAKVHLAPAGTPSAKLNGRLADA"
     gene            complement(10764..11153)
                     /locus_tag="VFR00_05090"
     CDS             complement(10764..11153)
                     /locus_tag="VFR00_05090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0158661.1"
                     /translation="MRVLGPVVTGVLACLSLLAVPAEAGEGGWSFGGRIEQLPEGQGC
                     YWFGGHLYCNRWCYWEIDGYRYCQRRVGAAISQAPPVLLYDFEPRRYDRIRRGPIVQD
                     NGPPPPPPRKRPIPRPGPDQGPLGPIK"
     gene            complement(11384..12700)
                     /locus_tag="VFR00_05095"
     CDS             complement(11384..12700)
                     /locus_tag="VFR00_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007592824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HEU0158662.1"
                     /translation="MAQQTPPDLATTRSRLEPALARRLLLGVTLITLMVVSVGGWAAT
                     TPIGGAVISSGFVVVESNIKKVQHPTGGIVAEISVKNGNVVNAGDIVISLDDTQARAN
                     LGIVVSQLVQLRGRKARLEAERDQADQVRFPVGFMESGEDAPAIMEGETRLFEVRQSV
                     KNGQIAQFTERIGQLNQEIKGITAQRDAKAEEVELMRDELERLEIMRKKDLVPTTRTL
                     QAQRDLTRLRGEWGANVAQIARSQGQISETELQIIGVTQNMQTDASKELREIEARIAE
                     LLERKVAAEDQLKRIFLRAPQTGFVHDLTVHTVGGVIGPGENVMTIVPTHDTLSVEAR
                     IGTSDIDQVAVGQQAVLRFPALNQRTTPEIRGTVTRVGADLTKDPQGNAVYFTLRISI
                     SDSDRETFKIVPGMPVEAFISTHERTALSYLMKPLSDQVTRAFRER"
     gene            complement(12709..>13751)
                     /locus_tag="VFR00_05100"
     CDS             complement(12709..>13751)
                     /locus_tag="VFR00_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU0158663.1"
                     /translation="LSDIVGSLTVVSRVFRLMLQSALLGLGAYLTISGEMTAGAIIAC
                     SIAASRALAPIEVAIANWRGFVAARQAAYRLETIFASLPKRTKPLLLQPPTSGLSIEG
                     LAVAVPGTQTITLSNITFAVKAGAVVAVIGPSAAGKSTLARAITGVWPLARGTVRLDG
                     AAHSSWSPEELGKHVGYLPQDVQLFDGTITENISRFEENPDSLKVITASQMACVHEMV
                     LRLPNGYETRIGTGGSVLSAGQRQRIALARALYGNPFLVVLDEPNSNLDSDGEAALIK
                     AIEGVKARGGIVIVIAHRPSVLGAADTVAVIGGGQLTAIGPKDEILRKVLRYPVTAPA
                     ALAVTADAKAQG"
BASE COUNT         2178 a         4571 c         4599 g         2403 t
ORIGIN      
        1 ctcggccggc tcaggccgcc gccagagcga tggcgcggcg atacatcgtc atcgccgcct
       61 cgggcgtgtt gtaggcggca tgcgcggtca atgtcacgcg ttcaagcagc cgcagcggct
      121 cgctcgccgg cggcggctcg gcggcgtaga cgtcggtggc atagtggcca atgcggcccc
      181 cccgcaccag ctccaccagc gccgcctcgt ccagcaagcc ggcccgggcc gtgttaacca
      241 ggatggcgcc cggcttcaag cgctcgagcc gaccgcggtc gaggaaatgg cgggtgccgt
      301 cgttaagggc caggtgcagg ctcaccacgt cggcgcgcgc cagcagggcc tcgagcgaga
      361 ccatgggcac gggcgcatcc ggcaggggcg agcgattgaa ggcgatcacc tccagcccga
      421 tgccctggcc caggcgcgcc atctcgcggc cgatgccgcc gaggccgatc agcccgagcg
      481 tcttgccctt gagctcgagg ccctgcatga cctgccagcc accctcgcgc atgatcttgt
      541 gcatgctggc gatgttgcgc gccgccgcga acacgagccc catggtgtgc tcggccacgg
      601 cggtatcgcc atagcccttg atggtggaga ccttgatgcc ggccgtcgcc gccgcggcga
      661 gatcgataaa gctgccggcc cccgtgccga ggaagacgat gtgtttgaga tcgcggcagc
      721 gcttgagggt ggccgcggtg aagtaggtgg catcgacgat cgtggtgtcg taacccgtca
      781 gcagacgggc aatgtcggcc tcggccacgg gtcccatatt gacgtcgatg ggcgcgtctt
      841 gcggcccgtg cacgcgctgc caaagccgat cgatgtcggg cgttgaatcg ataaagagcg
      901 tctttggcat ggcgccaacc ctcgattttg cggcgtgacg caggctcgcg ccgcgctgcc
      961 tcgaccataa ccgcccaagg ccggcgctgg gcaatggccg ggcgcgggtg ggcgggaagc
     1021 ggctggatgc ccgccgggga agaggctaac atccccctgc ggaaaaaaca cgggagccgc
     1081 gccagcgggc gccaggagga ggaacgcgga cgatgcaggg tcaattgcga atgcggggcc
     1141 gcatattgct gaagggggcc gccacggcga tgctggccgc gaccctgtgg gccaaccccg
     1201 ggtccgcgca ggagcgcgaa atcaaggtcg ggtacatgaa gaacccgatc caggacgcct
     1261 cgctcgacat gatggaaaag tgggccaagg ccaacaacgt caagctgacg cgcgaaccca
     1321 tggcctacag cgtgttcatg gagaaggtca cggcgacgct gacctcgggc gcagaccagt
     1381 tcgacatgat ctggcacaac gacgactggg gccagctctg gaagaaatgg gtcgagccga
     1441 ccgacgacat caagggcctc gataccgttg atcagtggcc gctgcttgcc ttctggaacg
     1501 acgacaagaa gctgaccgtc gtgccgatgg cgcacacggt cggcacgttc ttctaccgca
     1561 gcgatttggt gaagccggag gaggtgccga agaccttcgg cgagctggtt acggtcagcc
     1621 agcgcctgca gaaggaaggc aaggtcaagt ggggttatgt cggcggcatg accatgaaca
     1681 acacctggtt cagcttctgg tggaccatgt gggccaatca atgcgacatc ctcaagccgc
     1741 tgctcgagcg cgacaacgaa aagctgaagg cggccaaatt cgagcccgcc atcaccgagc
     1801 cctgccatcg cgagatcgtc gagtattggt gggatgccat caacacgcac aagatcagcc
     1861 cgccgggcat gacggcctac ggccgcaacg aggccaatgc catcttcatg gcgggtgacg
     1921 ccgccttcac gctggtcgat tccacgcact tcggcgaatt cagcgatccc aagcgctcca
     1981 agatcgtggg caaggtgagc atggcaccgt tccccgtcgg cccgcgcgcc aaagtcccca
     2041 cctcctggaa cgagatctgg ggttgggcca ttccgatcgg ggtgccggcc gagaagaaga
     2101 aaatcgtcaa ggacatgctg tcggccatga tgagcgacga ggccggccag atcgagatgt
     2161 ggaagaaaac cggcggaccg ccgccgaaca tcaagctgtg gccgaagctc gccgcggagg
     2221 acaaggactt cgccgccttg aagcacgccg tcttcgacca gaagccgatc acgcattccg
     2281 cctactactt tgccgagtgg ccggcggtgc acaaggccta ctcggacgcg gtcatcgagg
     2341 cgatctcagg caagcgcgag gacatcggaa aggtgctgga ggcccacgtc gagaaggttc
     2401 ggcgcgccgg ccttggcacc aactagcgct gtgccgggcc acgggtcagg ccccgtcata
     2461 gcgaggggca gccccgctat gacagcccgc gggcgcaccg gcggcagtcg cctgcgcacc
     2521 gcttcgcttc gcgcctccac attgcggtag cagctcgcat gccccagcgc agccttccgc
     2581 ggatcgatga gaagaagcgc ttcatgctgg cgctgatcgc gccggctgtg acggtgctga
     2641 tcgcctttca gatcctgccg atcttgatcg gcgccaatgc cagctttcgc gactggcatc
     2701 tgcacgaccc gaagcgaacc tggatcgggt tcacgaacta tgcctatgtc ctgcgcgaca
     2761 acgaattcct ggcaatcgtc ctgccgaata ccttcggctt catgctggcg agcgtgggca
     2821 cttccctcat tctcgggctc gccatcgccc tgctgctgaa ccgcaagttc gccgggcgct
     2881 ggctggtgca gaccatcatc ttgctgccgc tgatggtggc ccccgtcatc gccgccatca
     2941 tgatccgctg gatgttcaac gatcagttcg gcatcgtaaa cgtggtcctt ggtgtattcg
     3001 gcgtgccgca gatcgcctgg cttacccaga agtggacggc cttcctggcc atcctgctga
     3061 ccgacgtctg gttgtggacg ccctggttcg ccttgctgct gttggcgggc ttgcagagcc
     3121 tgccgcccga gcccttcgaa gccgcccaga tcgatgcggc cagcgcctgg cggacgtttc
     3181 gctacattac cttgccgatg ctgcgcccgg tgatcgtcgt gtgcatcgtc atccgtgcca
     3241 tcgatgcctt ccgcaccttc gacatcgttt ggaccatttc ggggggcggc ccggcgcggg
     3301 ccacggaggt gttcagcatt tatgcctacg tcgaggcctt tcagttcctg aacttcgggc
     3361 gcggctcggc cgccgccgtg atcggcgccc tcattatcgt ggtgtttgcc atggcgctct
     3421 atcgcctcct caaccgctgg gtggaggtgt cgcgatgagc gcggtcgagg ccttgccggc
     3481 acttgccagc cgcaaccgct tcttcgatgc cctggcgccc ggcggccggc gcgccttgtt
     3541 cgtgctgggc ctgatcggcg tttgcctgct gtttgcgttt ccagtgttct ggctggtgct
     3601 gacgtcgctg cggccgggct atgccgtcta ctacgtgcac cgcgggaccg atttcacgct
     3661 cgacaatttt cacgaggtcc tgagccaaga gatcgtgttg cgcgcgctgt ggaatagctt
     3721 tgccatctcg accctggcga ccgttctttc gctcggcgtg acggtgacgt cgggctacat
     3781 gctctcgcgc ttccggggtc cgatacccaa tctgtggttt tcggcaattt acgtgtttcg
     3841 ctgcgtgccc tacgtgtcgt gggtgctgcc cctctatttc gtcacccgcg ccctcggcat
     3901 cttcgatacc tactggggtc tgctgctgcc gcacgtggcc gtgcacatct gcttcttttc
     3961 ttggatcatg aaaggttttt tcgacggcat cgatccttcc atggaatacg ccgccatgat
     4021 cgatggttgc tcgcgctggg gcgcattcct gcgcgtggca ttgccggccg ccatacccgg
     4081 gctcaccgcg ctcgccatcc tatgctggct ctatacctgg aacgaatttt tgttcgcgct
     4141 cattctgacc gcgcagaaaa ccccgatcct gaccgtggtc atggcgcagt tcgtgcacga
     4201 gctcggcatg gaatggcatc tgatgagcgc aaccgcggtg ttgtcgctgg ggccggccct
     4261 gatcgtcacc atcttcggcc agaagtacgt cattcgaggc ttaaagatct aaaacatggc
     4321 cgaagtcacg ctcagcaatg tgcgcaagac ctatccgggc ggcgtcgccg ccgtgaagga
     4381 tttcaacctg cacatcgccg accatgaatt cgtcgtcttg gtgggaccgt cgggttgcgg
     4441 caagtccacg acgctgcgca tggtggcggg gttggaggac atcagcggcg gcaccatcgc
     4501 catcggcgac cgtatcgtca atggcctgca gccgaaggat cgtgacatcg ccatggtgtt
     4561 ccaaaactac gcgctctatc agcacatgag cgtgtacgac aacttggcct tcggcttgcg
     4621 caatcgtcgg gcgaaagagg ccgacatcga cgccgcggtg cgtggggcgg ccaagatcct
     4681 gtccatcgaa ccactgctcg agcggcggcc gcgccagctt tcgggcggcc agcagcaacg
     4741 cgtcgcgctg ggccgctgca tcgtgcgcaa cccgaaggtc tttctgttcg acgaaccgct
     4801 ctcgaacctc gacgcgcaat tgcgagcgca gatgcgcctg gagctaaaag agctgcgcca
     4861 gcgcgtgccg accacctcgg tgttcgtgac ccacgatcag gtcgaggcca tgacgctggg
     4921 tgaccgcatc gtggtcatga aggatggcct ggtgcagcag gtcggcagcc cgcttgccct
     4981 ctttcgccag ccgatcaatc gcttcgtggc gagcttcatc ggctcgccgg ccatgaattt
     5041 tctcgacatg gcggtggcgg cccaggccga cagcttccgg ctcacagccg agggcgtggc
     5101 catcgagctg ccgcagctgc gcttcccccg tctgggcgcg cacgtggggc aaagcgtgac
     5161 gcttgggctg cggccacagc acctgcgcct cggcagcgcc ggcaatggcg cccaggtcgg
     5221 cttcagcggc aacctgatgg tcaccgagca gctcggcgac gagcagctgc tggcagtgcg
     5281 catcggccgc ggcgatctgc gcgtttccgg tgtcgatccc gagctcgcgc tgacaacggg
     5341 gacccggatc gatctggtgg tgccgatcga aagcttgcac attttcgatg gcagcaccgg
     5401 agcggccatc cgctaggccg ctgccggcct cggcaactca tgaagggggc ggcgatgcaa
     5461 gggctcgctg gcagacgtgc gctggtcacc ggtgcgggca atggcatagg ccgggccatc
     5521 gccttcgaac tggcgcggca gggggcggcg cttgcgctcg tcgacatcga cggcccggcc
     5581 gcagaagcga cggcaaagga gttgcgctca agcggctgcg ccgtcgccgt ggccgtggcc
     5641 gatgtgtcgg acgcggccgc cgtcaaggcg gccgtgagcg ctttcgaggc cagcggcgag
     5701 ccgctcgaga tcctcgtcaa caacgccggg atcgcccgcc tcggctcgct gctcagcatc
     5761 agcgaacagg actggcgcga cacctttgcc gtcaacgtgg acggcatgtt ccacgccacg
     5821 cgcgcggtcg tgcccggcat ggtggcgcgc cggcgtggcg cggtggtcaa tctggcgtca
     5881 tggctcggcc gccgcggcaa cgccttgttc ggcgcctatg ccgcctcgaa gtttgccgtg
     5941 atcgggttga cgcagtcctt ggcggccgag gtggcgccct gcggtgtgcg ggtcaatgcc
     6001 gtgtgccccg gcatcatcgc cgatacgccc atgcgttcgg ccctcgaacg ggcaggcgca
     6061 gccgcgggcc tggctccgac cgaggcgcgc gtcaaggcca ttcccttgcg gcgcgcgggt
     6121 aggccggacg aggtcgccaa agtcgtggcg tttttggcct ccgacgcggc cgcctacgtg
     6181 acgggcgcgg cctatgacgt gtccggtggc cattggatga cgtgacgggc ccaagcctat
     6241 gacgtccctg ttcgagccgg gccgcattgg ccccgtgcaa gtgccgaacc gtatcgtgat
     6301 ggcgcccatg acgacgcggg ccgccgatcg cgaggggttc gtcacggcgc aaacccttgc
     6361 ctactacggc gcccgcgcgc gcggcggcgt tggcctgatt accgtcgaga tggcctcgcc
     6421 cgaacgctgt gggcgccatc gccagcacga gctcggcatt ttcgacgatc gcttcttgcc
     6481 gggcctggcg cggctggcga gcgcgatcca tgccgagggg gccaaagcct ccatacaact
     6541 tggacacgcg ggcggccata cgcgccccga tatctgcggc gagccgccga tcgcgccctc
     6601 cgccattccc cacctggtcg aggaggtcac gaccgagacc atcatccccg aaccgatgag
     6661 caagcagcgc atcgaggaga cgacggccca cttcgttgcc gcggctcggc gcgccggcgc
     6721 ggcgggtttc gattgtgtcg agatccacgc cgcgcacggc tatctcatct cccaattcca
     6781 caccccgttc gagaaccggc gcagcgacga gtatggcggc agcttgcaga accgcgcccg
     6841 cttcggtctc gagctgacac ggcgcgtgaa atccgccgtc gccgagctcg gcgtcatcta
     6901 ccgcttgacg gtcgatgact tcttcccgca agggctccaa ctcgaggagg ggctcagcat
     6961 cgccgagtgg gctcaacgga gcggcgccga tgctctgcac attgccgcgg gccactaccg
     7021 ttcgctgccg ggcacgcccg gcatgatccc gcccatggcg tggccggacg caccctttct
     7081 gaaatttgca gccgccctga agccgcgtgt ggcattgccc gtgatcgccg tgggccggct
     7141 aggggatccc tcgaccgccg cggcggcgtt gcgtcgaggc gcctgtgact tcgtggcgct
     7201 tggccgtgcc ttgctcgccg atgccgagtg ggcgcgaaag gtcaaaaccg gggcgcccgt
     7261 gcgtgcctgc ctcgcctgca atacctgcat cagcggcatg cgcggcggtg gcgcgctcaa
     7321 ttgcctcgtc aatgccgaaa ccggccggga ggccgctttt gccgcacgcg tgccgctacg
     7381 gggcaagcgc atcgccgtca tcggcgccgg cccggccggc ttgacctacg cctctctcgt
     7441 cggcggctcg aacgaggtcg tcgtgttcga gcgcgacgcc tggccagggg gcagcctgcg
     7501 cgacgccggg cgggcgccgc gctttcaagg cgtggcggca tcgcgggcgt ctctggcgcg
     7561 ctatgttgcg agcctggagg cggcatgcgc gcacgccggc gtgcggcttc gctacggcgt
     7621 caacgtcgcc caacgccccg aggtgttggc gggcttcgat cggctggtag tggcgaccgg
     7681 cgcggactat cgattgggcc tcggccggcc cctacgtgca ctgttgcgtt ggggtgtggc
     7741 gcgctggcct ggcgtgcgcg gactatttgc acggccatgg ctgctcgatt ttctctacta
     7801 ccgtgcgcgc cgcccgtcgg gcgcggccat cgcccgcttg gctccggccg gcaccgaggt
     7861 cgtcgtcatc ggcgatgcgg cagagcccgg caagtcggcg cgcgcgatcg agagcgcctt
     7921 tgccgcggcc tgggatggga cgctaggcgc ccagcgcaac caggcggctc agccgccgag
     7981 cgacgcggcc aagcccgccg caaccgagca tgagacgcat tgagactttt tgcaggcaat
     8041 gttgcggccg ctggcggttg ccaacagcga gggctgcagc tggcgccggc gctctctaat
     8101 ttgtgaacga gccgggcgcg cgcgctcgcg acggcgcgtg tgcccagttg cgctggggct
     8161 tgccacatct gccactgcct cgcaattgcg cagaaacccg gcacagttgc agctatccgt
     8221 tgcgcacacg ggttcagcgc tggtcttcgc cgcggaaatc gcaaatgatg ccgccgctgg
     8281 cactctccgg tgccttgcac ttaccccgca acgcgggcac cgtgactgcc acgtcaaagg
     8341 ctggctccca agcaccctgg gggccagttt ttctttcaat gcgcgcggta ccctgaggtt
     8401 tacgcgcctc gacgagctca gccgatcggt tagcatggcc tggcgtcgcc gctggcccga
     8461 gcgcgggctg ccggggccaa gtcggcgcgc ggcagcggtg cggtgtgggg aaggccagat
     8521 gcgtattgcg ctgtgcaatg aagtcgtccg cgaactttct ttcgagcgcc agtgcgcgtt
     8581 cgcgcgcgcg gtcggttacg acggtcttga gatcgcgccc ttcacgctcg gcgaggagcc
     8641 ccaccggctg gcgccgtcgc ggcgcagtgc cattcgggct gccgctgccg ccgagggtat
     8701 cgccatcacc agcctccact acctgctgct cgcgcccgcc ggcctgtcga ttacggccgc
     8761 ggcgggcgcg cagcacgaca ggacgatcga ggtgatgcgc cggctgtgcg atcttgccgc
     8821 cgatctgggc gccggcgtgc tcgtacatgg ctcgccggcc cagcgccagc tcgaggtggg
     8881 caaggaagcc gagggccggc agcgcgcgat tgccgctttt gccgcggccg ccgaggccgc
     8941 gggcagcgcc ggcgtcacct actgcatcga acccttggcg caaacggaaa ccgggttcgt
     9001 caataccatc gccgaggctg cagcgatcgt gcgcgaggtc gggcatgcag ccctcaagac
     9061 catgctcgat tgctcggccg cggcgcgcgc cgagccgcag gcgatccccg agctgctgcg
     9121 gcactggctg ccctcgggcc tcatcgctca cgtccactgc aacgatccca atcggcgcgg
     9181 gcccggcgag ggcagcctcg cctttgctcc catcctgcag gccttgcgca cgggcgctta
     9241 tcggggaatg gccgcggttg agccgttcgt ctacgaaccc gacgggcccg cgtgtgcggc
     9301 gagggcgatc ggctacctac gcggcttgca gcaaggcgcg ccggcccagg agacgcccag
     9361 gcccggcccc ggcgattccc tcggggaagg cgttggcggc ccgcaatggg ccggatgatc
     9421 ggattacccg tcggcgcgca cgaaaaaaaa cggcacgccg aagttaagcc catcctcgcg
     9481 gcggcgatgg tgacgtccgg ttcgagatgg ggctcgtcac agggaatggc gcgacaggcc
     9541 gtggcgcgcc aggtcatccc ggccggcgcg ctcaaggctc gcgcgccgga ctttgaggag
     9601 gatcgcccag cagatcgcca gcgcgcacag catcaggatc gaatggtact tgatctcgac
     9661 gccgcccagg acggagacgt cggtggtcgc gagattgccg atcaggccga ggtcgggcat
     9721 ccagtcgacg atgcgcagat aggcgaggta cccaacgagg agcgctagga cgatgatcgt
     9781 cgtctcggta tcggcctggc gcttcatggg acagctctca aaccgcgagc aggtgggcgc
     9841 tcgccacctg ctctaagtag tcccgcttcg tgccgttcgc caccaaccgc gaggcgtaaa
     9901 cgtgccaatg actggcagtg gcggtgccaa tgcatcccaa tcgcaagtgg ctcggctcag
     9961 gcggcgcgac gggccaagtg cgagggcatc ggcccgcggc ggccttgggc cgtgcgcctt
    10021 gagagcaaag gcgccttaag cgtccgccag acgcccatta agctttgccg agggtgtgcc
    10081 ggccggcgcc aagtgcacct tcgcggcgcg cggggatggg tagacggcga tcgcgaactc
    10141 caagtgccgc gggcggtctt cggcgacgat gtcctccagg atcatgatcg cctcgggcgt
    10201 gaaatcgacc agtgacgctt cggccagagc gagcagaaat tcatcgatcg gctgctgcag
    10261 atcgatctta gtgtcttggc tcatcgaggc ctctggggtc gcttccatca cgcaaacggt
    10321 tctgagcgcc attctattcc aatctgggcc gctgtctgcc tcctaagggc gggtggcttg
    10381 ggtctaccga ggcccccctc agcctacccc aaaagtccaa cgccccggga gcccgctgga
    10441 ccccgttggt ccagccgcgg cggcgtgtcg tggccgcctg ttgcgagcgc ttggcagtag
    10501 cgccttcacg ccccggggtt gcagccattt tgccgcagcc gaggcaagcc cccggcgcga
    10561 cccgcccatg cccggcggtg ccggcttggg ctcgattttc aaggcgttct gtggccgtgc
    10621 gcggggcgtg ctgcaggccc cttgggcggg gctcgcagcc gccgccaggc aggagcgttg
    10681 gcgcgcgcca aagcctgcgg ctgcccacac gctgcccggc ggggtcgagc gccgccccag
    10741 acaaccctcc tgatggatcg gccctatttg attggcccta gcggcccctg gtcggggcca
    10801 gggcgcggga tcgggcgctt tcgcggcggc ggtggcggag gcccgttatc ctggacgatc
    10861 gggccgcggc gaatgcggtc gtagcgccgc ggttcgaaat catagagcag cacgggggga
    10921 gcttgcgata tcgccgcgcc cacccttcgc tggcagtagc ggtagccatc gatctcccag
    10981 tagcaccagc gattgcagta aaggtggccg ccaaaccagt agcagccttg gccctcgggg
    11041 agctgctcga tcctgccgcc aaagctccac cctccctcac ctgcctcggc ggggacggcg
    11101 agaagcgaca agcacgcaag caccccggtg acgaccggtc ccagcacgcg cattgtccgt
    11161 tctccccttc gacggcgatc gcgccgcaga tccggccgcg ccaaagatcg cgttgcgcct
    11221 tccgaacacg ctagccgatt cgagtcctcg attcgaacag cccggtgact tcagcatacg
    11281 cgcgtcgcct gcccctggcg agccgatcgc cgccagggct cagaaaaaac tgcaaggtgg
    11341 gtcgaaagcg cctccctgcg cgggaggcgc tggggttact ggctcagcgc tccctgaagg
    11401 cgcgcgtaac ctgatcgctc agcggcttca tcaggtagga cagggctgtg cgctcgtgcg
    11461 tggaaatgaa ggcttccacc ggcatgcccg gcacgatctt gaaagtctcc cgatcgctgt
    11521 cggagatgct gatgcgcagc gtgaagtaga ccgcgttgcc ctggggatcc ttggtcaggt
    11581 ccgccccgac ccgggtcacc gtgccccgga tctccggcgt cgtgcgctgg ttgagtgcgg
    11641 ggaagcgcag cacggcttgc tggccgacgg caacctggtc gatatcggag gtgccgatgc
    11701 gagcctccac gctcagcgta tcgtgggtgg gcacgatcgt catcacgttc tcgccgggtc
    11761 cgatcacgcc gccgacggtg tgcaccgtca agtcgtgcac gaaaccggtc tgcggcgcgc
    11821 gcaggaagat gcgcttgagc tgatcctcgg ccgccacctt gcgttccaac agctcggcga
    11881 tgcgcgcctc gatctcgcgc agctccttgc tcgcatccgt ctgcatattt tgtgttacgc
    11941 cgatgatctg caattccgtt tcgctgatct ggccctgcga gcgcgcaatc tgggcgacgt
    12001 tggcgcccca ctcgccgcgc aagcgtgtca gatcgcgctg ggcctgcagc gtgcgcgtgg
    12061 tcgggaccag atccttcttg cgcatgatct ccaggcgctc gagctcgtcg cgcatgagct
    12121 cgacctcttc ggccttggca tcgcgttgtg ccgtaatgcc cttgatttcc tggttgagct
    12181 ggccaatgcg ttcggtgaac tgggcgattt gcccgttctt cacggattga cgcacctcga
    12241 agaggcgcgt ttcgccctcc atgatggcgg gggcatcctc gccgctttcc atgaagccga
    12301 cggggaagcg gacttgatcg gcttggtcgc gctctgcctc cagacgcgcc ttgcgaccgc
    12361 gcagctgcac cagctgcgac acgacgatgc ccaggttggc gcgggcttgc gtgtcatcga
    12421 gactgatcac gatatcgccg gcattgacca cattgccgtt cttgaccgag atctcggcga
    12481 cgatgccgcc cgtcggatgc tggactttct tgatgttgct ctcgaccacc acaaagcccg
    12541 aggagatcac ggctccgccg atgggcgtgg tggcggccca accaccaacg ctgacgacca
    12601 tcagggtgat cagggtgacg ccgaggagca gacggcgcgc cagcgcaggc tcgagcctgc
    12661 tacgcgtcgt tgccaagtcg ggaggggtct gttgtgccat tgcgggcttc atccttgtgc
    12721 cttggcgtcg gcggtgaccg ccagcgcggc gggcgcggtc accggataac gcaagacctt
    12781 gcgcagaatt tcgtccttgg gaccgatcgc cgtcagctga ccaccgccga tcacggcgac
    12841 ggtgtcggcg gctcccagca cgctcggccg atgcgcgatc acgatgacga tgccgccgcg
    12901 cgccttgacg ccttcgatgg ccttgatcag cgccgcttcc ccatcggaat cgaggtttga
    12961 gtttggctcg tccagcacca ccaggaacgg gttaccgtag agggcgcggg caagcgcgat
    13021 gcgctggcgc tgtccggccg acagcaccga gccgccggtg ccaatgcgcg tctcataacc
    13081 gttcggaagc cgcagcacca tctcgtgcac gcaggccatc tgcgaggccg tgatcacctt
    13141 gaggctgtcg ggattctctt cgaagcgcga gatattctcg gtaatggtgc cgtcgaagag
    13201 ctgcacgtct tgcggcaggt aacccacgtg cttgcccagt tcctccggcg accagctgga
    13261 gtgcgcggcg ccatcgagcc gcaccgtccc ccgcgccagc ggccaaactc cggtgatggc
    13321 acgcgccagc gtcgatttgc cggccgcgct cgggccgatc acggccacga ccgcgccggc
    13381 tttgaccgcg aaggtgatat tgctgagcgt gattgtctgg gtgccgggaa cggccaccgc
    13441 caagccttcg atgctgagcc cgctggtcgg gggctgcaga agcaacggct tggtgcgctt
    13501 gggcaggctg gcgaaaatcg tctctagccg gtaggcggcc tggcgcgccg ccacgaagcc
    13561 gcgccagttc gcaatcgcca cctcgatcgg cgccaaggcg cgcgaggccg caatcgagca
    13621 ggcgatgatc gcgcccgcgg tcatttcgcc cgagattgtc agataggcgc cgaggcccaa
    13681 cagggcggat tgcagcatga ggcggaacac gcgcgagacg accgtcaaac tgccgacgat
    13741 gtcgctcaag c
//
LOCUS       DASTMT010000216         2515 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_443231, whole genome shotgun sequence.
ACCESSION   DASTMT010000216 DASTMT010000000
VERSION     DASTMT010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2515
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_443231"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1088)
                     /gene="glcF"
                     /locus_tag="VFR00_05105"
     CDS             complement(<1..1088)
                     /gene="glcF"
                     /locus_tag="VFR00_05105"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HEU0158664.1"
                     /translation="MQTNFSARQLQDPRTAEVDKILRRCVHCGFCTATCPTYLLLGDE
                     RDSPRGRIYLMKEMFETERKASPEVQRHIDRCLSCLSCMTTCPSGVDYMHLVDHARVH
                     IEATGKRSLKDRALRALLAAVLPYPDRFRLALRAARFAKPLSGLLRRLGLRELVAMLE
                     LAPIGLLRGAAFAGPGTAITKLERRKRVILLAGCAQQVLRPEINDATIRLLARRGVDV
                     EVAPGAGCCGGLVAHMGREAAAIAMAKRNVDAWSRLIEKAGPIDAVIVNTSGCGTTVK
                     DYAHLLKGEAGYLERAKRIADMTRDLTEFLAGYEMGPPKRWSSLRIAYHAACSMQHGQ
                     RISEEPRKLLRNAGFTVVEIPEGHLCCGS"
     gene            complement(1100..2320)
                     /gene="glcE"
                     /locus_tag="VFR00_05110"
     CDS             complement(1100..2320)
                     /gene="glcE"
                     /locus_tag="VFR00_05110"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013949419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HEU0158665.1"
                     /translation="MLSDVRPVDEREVARILREAGQAKTPLELRGGGSKHNVGRPMTT
                     SMCLSTRALNGITLYEPNEMVMAARAGTLLIELEDSLAQRGQMLAFEPTSLAAVCGAE
                     ARSATIGAVFATNLSGARRITVGAARDHLLGVRAVNGRGEIFKAGGRVMKNVTGVDLC
                     RGLAGSWGTLAVLSEVTFKVLPLPEHTETLVLLGLPDEIAIEVLGSAMGTPFEVSGAV
                     HLQPAMVARLEHQGLAAQGQAVTALRLENFRRSVVYRKAKLKAHLKAYGEIHDLDHDN
                     SLALWQELRGLALLANSEAPLWRISTAPSTGPNVVAAVARYMEVGAFYDWAGGLIWLE
                     IPPTTDAGAADIRRVIATHGGHATLIRAGAEVRGTVDVFQPLEAGVEVISRRLKAAFD
                     PAGILNPGRMYADF"
     gene            complement(2332..>2515)
                     /locus_tag="VFR00_05115"
     CDS             complement(2332..>2515)
                     /locus_tag="VFR00_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007565362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HEU0158666.1"
                     /translation="EHGVGIEKRDLMRVQFDASDLEQQMRVKSVFDPSWLLNPAKVFP
                     LQGRPQAEPKPAVTAA"
BASE COUNT          404 a          870 c          835 g          406 t
ORIGIN      
        1 gagccgcagc acagatgccc ctccgggatt tcgacgaccg tgaagccggc attgcgcagg
       61 agcttgcggg gttcctcgct gatgcgctgg ccatgctgca tggaacaggc cgcatgatag
      121 gcgatccgga gcgaggacca gcgcttgggc ggtcccattt cgtagccggc caggaactcc
      181 gtgaggtcgc gcgtcatgtc ggcgatgcgc ttggctcgct ccaggtagcc ggcctcgccc
      241 ttgagcaggt gggcatagtc tttgacggtc gtgccacaac ccgaggtatt gacgatcacg
      301 gcgtcgatgg ggccggcctt ctcgatcagc cggctccagg catcgacgtt gcgcttggcc
      361 atggcgatgg cggcggcttc gcggcccatg tgggccacca gcccgccgca acagcccgcc
      421 cccggcgcca cctcgacgtc gacgccgcgg cgggccaaga gacggatggt ggcgtcgttg
      481 atttccgggc gcagcacctg ttgggcgcag ccggcgagca agatcacgcg cttgcgccgc
      541 tcgagcttgg tgatggccgt gcccggcccg gcaaaagccg ccccacgcaa gagcccgatc
      601 ggcgccaact ccagcatcgc caccagctcg cgcaacccga gccggcgcaa caggccgctc
      661 aagggcttgg caaagcgtgc cgcccgcagc gccaagcgaa accggtcggg gtagggcagc
      721 acggccgcca gcaacgcgcg cagcgcccgg tccttgaggc tgcgctttcc cgttgcctcg
      781 atgtgcaccc gcgcatggtc gacgagatgc atgtagtcga ccccgctcgg acacgtcgtc
      841 atgcacgaca aacagctgag gcagcgatcg atgtggcgtt gcacctccgg gctggccttg
      901 cgctcggtct cgaacatctc cttcatcagg tagatgcgcc cgcgcgggct gtcgcgctcg
      961 tcgcccaaca aaaggtaggt cggacaggtt gcggtgcaga agccgcaatg cacgcagcgg
     1021 cgcaggatct tatcgacttc cgcggtgcgc gggtcttgca gttgtcgggc gctgaagttc
     1081 gtctgcatgg tgttcgcgtt caaaaatccg catacatgcg gccggggttc aggatcccgg
     1141 ccggatcgaa ggccgccttc aggcggcgcg agatgacctc gacgccggcc tccaacggct
     1201 gaaagacgtc gaccgtgccg cgcacctccg cgccggctcg aatcaaggtg gcgtggccgc
     1261 cgtgcgtggc gatcacacgc ctgatgtcgg cggcgcccgc atccgtggtc ggcgggatct
     1321 cgagccagat cagcccaccc gcccagtcgt aaaaggcgcc cacctccata tagcgcgcga
     1381 cggccgcgac cacattggga ccggtgctcg gcgccgtcga gatacgccac aggggcgcct
     1441 cgctgttggc cagcagcgcg aggccgcgca gttcctgcca gagcgccagg gagttgtcat
     1501 ggtccagatc atggatctcg ccgtaggcct tgaggtgagc cttgagcttg gccttgcgat
     1561 agaccacgga gcgcctgaag ttctccaacc tgagcgcggt gacggcctgg ccttgagcgg
     1621 cgaggccttg gtgctcgagg cgggccacca tggcgggctg caggtgcacg gccccgctca
     1681 cctcaaaggg cgtgcccatg gccgacccca gcacctcgat ggcgatctca tcgggcagac
     1741 cgagcagcac cagcgtctcg gtatgctcgg gcaacggcag caccttgaag gtcacctcgc
     1801 tcaggacggc cagcgtgccc cagctgccgg ccaggccgcg acagaggtcg acgcccgtca
     1861 cgtttttcat gacgcggccg ccggccttga agatttcgcc gcggccgttc accgcccgca
     1921 cccccaacag gtggtcgcgc gccgccccga ccgtgatacg ccgggccccg gagaggttgg
     1981 tggcgaagac ggcgccgatg gtggcgctgc gcgcctccgc gccgcagacg gcagccaagc
     2041 tggttggctc gaaggccagc atctggccgc gctgggcgag gctgtcttcc agttcgatca
     2101 gcaaagtgcc cgcgcgcgcg gccatcacca tctcgttggg ctcataaagc gtgatgccgt
     2161 tgagggcccg cgtggagagg cacatcgatg tcgtcatggg ccggccgacg ttatgcttgc
     2221 tgccgccgcc ccgcagctcg agcggggttt tggcctgtcc cgcctcgcgc aaaatccgcg
     2281 ccacctcgcg ttcgtcgacc gggcgcacgt cgctcagcac caatcgcccc ctcaggccgc
     2341 cgtcacagcc ggtttcggct cagcctgcgg gcgcccttgc agtgggaaca ccttggccgg
     2401 attgagcagc cagctcggat cgaacacgct tttgacgcgc atctgctgct ccagatcgct
     2461 ggcgtcgaac tggacgcgca ttaaatcccg cttctcgatc ccaacgccat gctca
//
LOCUS       DASTMT010000217         5993 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_4051117, whole genome shotgun sequence.
ACCESSION   DASTMT010000217 DASTMT010000000
VERSION     DASTMT010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 5993)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5993)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5993
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4051117"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..319
                     /locus_tag="VFR00_05120"
     CDS             <1..319
                     /locus_tag="VFR00_05120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEU0158667.1"
                     /translation="KNETMGSALGVLKEVLVKMAQDGPNPSQFENAKRYLTGSFLLDF
                     DTNAKVASSLLGIWVDGDSPDYLQARNQKINAVTLADVKRVAREVLKTQALIVTIVGK
                     PQL"
     gene            complement(337..1953)
                     /locus_tag="VFR00_05125"
     CDS             complement(337..1953)
                     /locus_tag="VFR00_05125"
                     /inference="COORDINATES: protein motif:HMM:NF025068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M12 family metallo-peptidase"
                     /protein_id="HEU0158668.1"
                     /translation="MIRARLLARLPLLLLPWLVSGTALAQGSKDSVEVIVSQSDASPH
                     TRMSVRGFFNLVKRTAASLTGFALPLTNCERWTLPKDKLDAVKKSAAKQGLLVTEVGA
                     SWDHVFQATPANVKITQKQQSLIDLAKGGKATTGVKIMAGPSPSMLEYALTKDAKAAA
                     GTEAPKLTVALSDQTVLTVARTSLDLKPKLCIWRGEVEQTGGLVTLMWWPNGRMAGTV
                     QDQGKIYSIRHMGGQLYAVVELNTERMPQEHASLPSSVRASDPNLRDDPLVQQGDASL
                     LRRLVTAMRRPQAATAPAQGAPHKTAAAAAPDARAAKAGARSKDVVIDVIVAYTRKAA
                     SNYGDVKRELVELAIEEANHSLRMSNLGNIKLRLVHAYQTSYVEEGQHFQHLWRFADR
                     GDGYMDEIHALRDKYRADVAVLIVDDPAGCGLSTRVYADADEAYSVVHHECAAASYSL
                     AHEIGHLIGARHELALDKTMTPFPYGHGFVNGSKWRDIMSYKVSCGGCPRLPVWSSPK
                     LLVKGEPAGSAEIDNARVISEQAARVAAFR"
     gene            2232..2606
                     /locus_tag="VFR00_05130"
                     /pseudo
     CDS             2232..2606
                     /locus_tag="VFR00_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010976062.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
     gene            complement(2706..3680)
                     /locus_tag="VFR00_05135"
     CDS             complement(2706..3680)
                     /locus_tag="VFR00_05135"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEU0158669.1"
                     /translation="MHYTTLGGTQLKVSVAGLGCGGNSRIGLGVGLSEAQAVALVQEA
                     LDLGVNLLDTAAAYGTEAVVGKAIKGRPRDRIVISTKSHVSANNRALSGSEVVGNLEN
                     SLRQLDTDYVDVFHLHGLAPRAYDHAMAEIVPQLLRQKQAGKLRHLAVSETGPRDPEH
                     KMLERASQKGPWEVVMLAFHMLNQNARRPVLEQTRANAIGTLCMFAVRNIFSKPGLLQ
                     TTVADLIAEGKLGKDVDQDNPLGFLVHPGGARSIVDAAYRFARHEPGIDVVLFGTGNV
                     EHLKSNIASILSPPLPREDVARLARLFGGLVGVGLDLPDRMREQATKR"
     gene            3723..5435
                     /locus_tag="VFR00_05140"
     CDS             3723..5435
                     /locus_tag="VFR00_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006022973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEU0158670.1"
                     /translation="MNLEARKSSCTTSHSEDSRGLDFYGSDPSLRGMLQIYLAAPERA
                     HLEPYLRQLGRLVGGRLDDLAREADKTPPRLEPRSRQGEDRQTIVKAPAYLAMERIAF
                     GELALAALSHRPALGWPQPLASASKYALTYLFAQSEFGLLCPVNMTDSLTRTLIRYAD
                     KALLARYLPGLLAEAGEDQLQGAMFMTERWAGSDVGATETRAARAGEAWRLYGEKWFC
                     SNADADLALVLARPEGAPAGTAGLGLFLMPRLLPTGAPNGYRIVRLKDKLGTRAMASG
                     EIVLDGAVAWLVGDLSQGFKQMAEMVNQSRLSNGVRSTGMMRRAVHEALTMALGRRAF
                     GQRLIDLPLVRRQLIKMILPAEAALSVAFFTADCLQRADRGDAEAQTLRRVLTPLIKL
                     RACRDARKLTGDAMEMRGGNGYIEEWIEPRLLRESHLGSIWEGTSNIIALDVVRAAAK
                     AAAHRLLEAQLQPFLAEAPSELGLNLKERLSAALSLLERAGAGAPDATVLARQAASGL
                     YYATAAALFLREARLLQDPVREDLARLLLSHKLNARDPLAATGEDDARGERVIAQLVA
                     ARQA"
     gene            complement(5457..>5993)
                     /locus_tag="VFR00_05145"
     CDS             complement(5457..>5993)
                     /locus_tag="VFR00_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006022397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEU0158671.1"
                     /translation="VILMQPGQFAWQVFDNKIIPMLRDEYRIKQVTKVRADTLEELVG
                     KLDDVNPSQALSTIKAYNAAVKKDVPFNPNIKDGRGTVGLAIPKSNWAIVLDTPPFEA
                     YAVTCGLTFTFGGLKIDGTARVINTDGEVIPGLYAAGELVGGLFYFNYPGGSGLMNGA
                     VFGRIAGTAAGQRAAQSP"
BASE COUNT         1019 a         1897 c         2003 g         1074 t
ORIGIN      
        1 gaagaacgag acgatgggct cggcgctcgg cgtgctgaag gaggtcctcg tcaagatggc
       61 gcaggacggg ccaaatccca gccaattcga gaatgccaaa cgctacctta ccggctcctt
      121 cctgttggat ttcgatacca acgcgaaggt ggccagttcg ctgctcggca tctgggtcga
      181 tggcgacagc cccgactacc tgcaggcgcg caatcaaaag atcaacgccg tgacgctcgc
      241 cgacgtcaag cgggtcgcgc gcgaggtgct gaaaacccaa gccttgatcg taacgattgt
      301 cggcaagccg cagttgtgaa gggctggcgc gctttattag cggaacgcgg ccacgcgcgc
      361 ggcttgttcg gagataacgc gtgcattgtc gatttcggcg ctgccggcgg gctcgccctt
      421 gacgaggagt ttggggctcg accacacggg caagcgcgga catcccccgc aactcacctt
      481 gtagctcatg atatcacgcc atttgctgcc attgacaaag ccatgcccgt aaggaaatgg
      541 cgtcatggtc ttgtccaagg ccaactcgtg gcgggcgccg atcaggtgac cgatttcgtg
      601 cgccagcgag taggatgcgg cggcgcactc gtgatgcacc accgagtagg cctcgtcggc
      661 atcggcgtag acccgcgttg acaggccgca gccggcgggg tcatcgacga tcaatacggc
      721 cacgtccgcg cggtacttgt cgcgcagcgc atggatctca tccatgtagc cgtcaccccg
      781 gtcggcgaag cgccacaagt gctggaagtg ttggccctct tcgacatagc tcgtctggta
      841 ggcatgcacg agccggagct tgatgttgcc cagattgctc atgcgcagcg agtggttggc
      901 ttcctcgatg gcaagctcga ccaactcgcg tttgacgtcg ccatagttgc tggccgcctt
      961 gcgcgtgtag gcgacgatga cgtcgatcac gacgtccttg cttcgcgccc cggccttggc
     1021 cgcgcgcgca tccggggcgg cagcggccgc ggtcttgtgc ggggcgccct gcgcgggcgc
     1081 ggtggcggct tgcggtcggc gcatggccgt caccaagcgg cgcagcaggc tggcatcgcc
     1141 ctgctggacc agcggatcgt cgcgcaaatt ggggtcgctc gcgcgcacgc tcgagggcag
     1201 cgaagcgtgc tcctgcggca tgcgctcggt gttcagttcc accacggcgt agagttggcc
     1261 gcccatgtgg cggatcgagt agatcttgcc ctgatcttgc accgtgccgg ccatccggcc
     1321 gttcggccac cacatcaagg tgacgaggcc gcccgtctgc tccacttcgc cgcgccagat
     1381 gcacagcttg ggtttgaggt cgaggctggt gcgggcaacc gtcagcaccg tctggtcgct
     1441 gagcgccacc gtaagtttgg gcgcctcggt ccccgctgcg gccttggcat ccttggttag
     1501 ggcatattcc aacatggagg gggacggtcc cgccatgatc ttgaccccgg tcgtggcctt
     1561 gccgcctttg gcgagatcga tcagggattg ttgtttctgc gtgattttga cattggccgg
     1621 cgttgcctgg aagacgtgat cccagctggc gccgacctcg gtgaccagca aaccctgttt
     1681 ggcggctgat tttttgacgg cgtcgagctt gtccttgggc agcgtccacc tctcgcaatt
     1741 ggtgagaggc aaagcgaagc ccgtcaggct tgccgcggtg cgcttgacga ggttgaagaa
     1801 gccgcgcacg gacatgcgcg tgtggggtga ggcatcggac tgggagacga tgacctcgac
     1861 gctgtccttc gacccctgag caagtgcggt gccgctgacg agccagggga gcagcagcaa
     1921 aggcagtcga gcgaggaggc gtgcgcgtat catggtgccc tgatcccgga cggtcgcggc
     1981 tggtttgcga aagctctcca acgcctcttg agttccagcc cggcaattta tcgtgccggc
     2041 gccggcgccg ctagcgtggg cgcacgcact atcttaagct cgaaatctca agctcgacgc
     2101 gcacgtcgat cgcgtcgcga agcatcaagc gtgtggcgcc cgcggcaaaa tccagtccac
     2161 cgccgggcgc cggtgaaagc agaaagcaga aagcagaaag cagaaagaaa ggtcaggcgg
     2221 gacaagccgt tatgccgaag caagcatcgc ggttaagatc cggcgcccgc ctcggcgtca
     2281 agcgcgttta tgcgccagcc gaggcgcagg acggcacgcg cattctggtc gaccgcttgt
     2341 ggccgcgcgg gatcgccaag gataaggcaa agatcgacat ctggctcaag gacatcgccc
     2401 caagcgacgc gctgcggcgt cgcgtgcacg ccaaggacgt gagctgggat gagttcgtgc
     2461 tcgagtatgg ccgcgagctg gcgcgggaac ccggcgccag cgcggcccaa gagcttattg
     2521 cgcgcatggc tcgcgagccg gtgacgctgc tctatgccgc ccgcgacgag caaaacaaca
     2581 acgccattgc gctcaagctt tggctcacga acacggctgg gcgggctcag ggcgcaggcc
     2641 cgccgggctc agtccggccg ccggccaaaa aggcggcccg gcgccgaggc ggcaaggatt
     2701 gatcttcagc gctttgttgc ttgctcacgc atgcgatcgg gcaggtcgag gccgacgccg
     2761 acgaggccgc cgaacagccg cgccaggcgc gcgacatctt cgcgcggcaa cggtgggctg
     2821 aggatcgagg cgatattgct cttgaggtgc tcgacgttgc cggtaccgaa caggaccacg
     2881 tcgatgcccg gttcgtgccg ggcaaaacgg taggcggcat cgacgatgct tcgcgccccg
     2941 cctggatgca ccaggaagcc caagggattg tcctgatcga catccttgcc gagcttgccc
     3001 tcggcgatca ggtccgcgac ggtcgtttgc aataggccgg gcttagagaa gatgttgcgc
     3061 accgcaaaca tgcacaaggt tccaatcgcg ttggcgcgcg tttgctccag aaccggccgg
     3121 cgtgcgttct ggttcagcat gtggaaggcc agcatgacga cctcccaggg acctttctgg
     3181 ctggcccgtt cgagcatctt gtgctcggga tcgcgcgggc ccgtctcgga gacggccaga
     3241 tggcgaagct tgcccgcctg tttttgccgc agcagctgcg gcacgatctc ggccatggcg
     3301 tggtcatagg cccgcggcgc cagcccgtgc aggtggaaga catcgacata gtcggtgtcg
     3361 agctggcgca gcgaattctc gagattgcca accacctccg agccggaaag ggcgcgatta
     3421 ttggcactca cgtgactttt ggtcgagatg acgatgcggt cgcggggtcg gcctttgatg
     3481 gccttgccga cgacggcttc ggtgccgtag gcggccgccg tatccaacag gttgacgccg
     3541 agatcgagcg cttcctgcac cagggccacc gcttgcgcct cgctgagccc gaccccaagc
     3601 ccaatgcggc tgttgccgcc gcagccgagc ccggcgacgc tgactttcaa ttgtgtccca
     3661 ccaagcgtgg tatagtgcat ggtcttgaac ctgagcgcat gaagcctaag cgattaaagg
     3721 ccatgaacct cgaggcacgc aagtccagtt gcacgacctc tcactcggag gactcccgtg
     3781 gcctcgactt ttatggctcc gatccatcgc tcaggggcat gctgcagatt tatcttgccg
     3841 cgcccgagcg cgcgcatctt gagccctatc tgaggcaact cggtcgcctc gtcggcggcc
     3901 ggctcgacga tttggcacgc gaagccgaca agacgccgcc ccggctcgag ccgcgcagcc
     3961 gtcagggcga ggatcgccaa acgatcgtca aagcccccgc ctacctcgcc atggagcgca
     4021 ttgcctttgg cgaattggct ttggcggcgc tctcgcaccg cccggcgctg ggttggccgc
     4081 aacccctggc gtcggccagc aaatacgccc tgacctatct gttcgctcaa tccgagttcg
     4141 gtctgctctg tcccgtcaat atgacggata gcctgacccg cacgctcatt cgctacgccg
     4201 ataaggcatt gctcgcccgc tacctgccgg gcctgctggc ggaagctggc gaggatcagc
     4261 tgcaaggcgc aatgttcatg accgagcggt gggccggctc ggacgtgggg gcgaccgaga
     4321 cgcgggcggc gcgcgcgggg gaggcgtggc ggctctacgg tgagaagtgg ttttgctcca
     4381 atgccgatgc cgatttggcg cttgtcctgg caagaccgga aggggcgccg gccggcacgg
     4441 cggggctcgg gctgttcctg atgccgcgcc tgctgcccac gggcgcgccc aacggctatc
     4501 gcatcgtccg gctcaaggac aagctcggca cgcgcgccat ggcctcgggc gagattgtgc
     4561 tcgacggcgc cgtggcctgg ttggtcggcg atctcagcca gggcttcaag cagatggccg
     4621 agatggtgaa ccagtcgcgc ctgtcgaacg gcgtgcgctc gaccggcatg atgcgccgcg
     4681 ccgtgcacga ggccttgacc atggcgctgg gacggcgagc ctttggccag cgcctgatcg
     4741 acctgccgct ggtgcgccgg caactcatca agatgatcct gccggccgag gccgcgctca
     4801 gcgtggcgtt tttcacggcc gattgtttgc agcgcgccga tcggggcgac gcggaagccc
     4861 agacgctgcg ccgtgtgctg acgccgctca tcaagctgcg ggcctgccgc gacgcgcgca
     4921 agctcacggg cgatgccatg gaaatgcgcg gcggcaacgg ctacatcgag gagtggatcg
     4981 agccgcggct tttgcgcgaa tcccacctcg gttcgatttg ggagggcacc agcaacatca
     5041 tcgccctcga cgtcgtgcgc gctgccgcca aggccgcagc ccatcgcctg ctagaggcgc
     5101 agctgcaacc cttcctggcc gaggcgccga gcgagctggg gctcaacctg aaagagcgct
     5161 tgagcgcggc cctgtcgctc ctcgagcgcg ccggcgctgg cgcgcccgat gcaacggtgc
     5221 tggcgcgaca ggcggcgagc ggactttatt acgccaccgc ggcggcgttg tttttgcgcg
     5281 aggcgcgttt gttgcaggat cccgtgcgcg aggatctggc gcgactgctc ctatcgcaca
     5341 agctcaatgc ccgtgatccc ctcgccgcga caggggagga tgatgctcgc ggcgagcggg
     5401 tgattgccca gctggtggcc gcccggcaag cctgacggcc gagccggcag gcgccttcac
     5461 gggctttggg ccgcgcgctg gccggctgcc gtgccggcga tgcgcccgaa cacggccccg
     5521 ttcatcaggc cgctgccgcc ggggtagttg aaatagaaca ggccaccgac caactcgccg
     5581 gcggcgtaaa gcccggggat cacctccccg tcggtgttga tcacgcgcgc ggtgccatcg
     5641 attttcaggc cgccaaatgt gaaggtcagg ccacaggtga cggcataggc ttcaaagggc
     5701 ggggtgtcga gcacaatggc ccagttggat ttgggaatgg caagaccgac ggtgccgcgc
     5761 ccatccttga tgttcggatt gaacggcacg tccttcttca ccgccgcatt ataggccttg
     5821 atggttgaga gcgcctggct cgggttcacg tcgtcgagct tgcctaccag ctcctcgagc
     5881 gtatcggcgc gaaccttggt cacctgcttg atgcgatatt cgtcgcgcag catcgggata
     5941 attttgttgt caaatacctg ccaggcgaat tggcctggct gcatcaagat cac
//
LOCUS       DASTMT010000218         2218 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_4982294, whole genome shotgun sequence.
ACCESSION   DASTMT010000218 DASTMT010000000
VERSION     DASTMT010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2218)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2218)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2218
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4982294"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1189)
                     /locus_tag="VFR00_05150"
     CDS             complement(<1..1189)
                     /locus_tag="VFR00_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019404730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HEU0158672.1"
                     /translation="MVSFDAIVIGTGQAGPSLAHRLAETGQRVAIIERHHFGGTCVNT
                     GCTPTKTLVASAYAAHLARRAGDFGVSITGEVKIDMRAVKARKDEVLGYSRRAIERGL
                     KAQSNISVYQGHARFAAAHSVEVGGETLNAPKIFINVGGRAFVPPIPGLEQISYLTNS
                     SLLEVESVPPHLIIVGGSYVGLEFAQVFRRFGAKVSVIEAAPRLIAREDEDTSAAVAD
                     ILGREGIEVFLGAKDLTFEKRGPDVVARLQSGGAAREIVGSHVLIAVGRRPNTDDLGL
                     EAAGVATDAKGFIKVDDQLQTSVAGIWALGDCNGKGAFTHTSYNDGEIVAANLLDGEA
                     RRVSDRITAYNLYTDPPLGRIGASEAEVRQAGRPALKATLAMEDVSRAFEKGETLGFM
                     KVLV"
     gene            1930..>2218
                     /locus_tag="VFR00_05155"
     CDS             1930..>2218
                     /locus_tag="VFR00_05155"
                     /inference="COORDINATES: protein motif:HMM:NF012862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HEU0158673.1"
                     /translation="MLALAVVMVGLPALASTVLADKRVALVIGNAAYEHTPKLNNATR
                     DADAMAALFKRAGFEVVLEQTNVGNLEFKRSLRNFLLAAQDADIAVLFYSGH"
BASE COUNT          382 a          724 c          700 g          412 t
ORIGIN      
        1 cgaccagcac tttcatgaag cccagggtct cgcccttttc gaaggcgcgg ctcacatcct
       61 ccatggcgag ggtagccttg agcgccgggc gcccggcttg ccgcacctcg gcctcgctgg
      121 cgccgatgcg gccaagcggc gggtccgtgt aaaggttgta ggcggtgatg cgatcgctga
      181 cgcgacgcgc ctcgccgtcg agaagatttg ccgccacgat ctccccgtcg ttgtaggagg
      241 tatgcgtaaa ggcgcctttg ccgttgcaat cgccgagcgc ccagatgccg gccacactcg
      301 tttgcaactg gtcatcgact ttgatgaagc ccttggcgtc ggtggcgacg cccgcagcct
      361 cgaggccgag gtcatcggtg ttgggcctcc gtcccaccgc gatcagaaca tgcgagccca
      421 cgatctcgcg cgcggcacca ccactctgga ggcgggccac aacgtcgggc ccgcgcttct
      481 caaaagtgag atctttggcg ccgagaaaga cttcgatgcc ttcgcgcccg agaatatcgg
      541 caacggcagc cgaggtgtcc tcgtcctcgc gcgcgatcag gcgcggtgcc gcctcgatga
      601 cgctgacctt ggctccgaag cggcgaaaga cctgcgcgaa ttcaaggccg acgtagctgc
      661 caccgacgat gatgagatgc ggcggcacgc tctccacctc gagcaggctg ctgttggtga
      721 gataggaaat ctgctcgagc ccgggaatgg ggggcacaaa ggcgcggccc ccgacattga
      781 tgaagatctt gggcgcgtta agcgtctcgc ccccaacctc tacgctatgc gcggcggcaa
      841 agcgggcgtg accctggtaa acgctgatgt tggactgcgc tttgagcccg cgctcgattg
      901 cgcgccgcga gtagccgagc acctcgtcct tgcgcgcctt caccgctcgc atatcgattt
      961 tgacctcccc ggtgatcgaa acgccaaagt cgccggcgcg gcgggcgaga tgggcggcat
     1021 aggcactggc caccagcgtc ttggtgggcg tgcagcccgt attcacacac gtgccgccga
     1081 aatgatgccg ctcgatgata gcaacgcgct ggcccgtctc ggccaggcga tgggcgagtg
     1141 agggaccggc ctgccccgtg ccgatgacaa tagcgtcgaa gctgaccatg gctgtacctc
     1201 cttggcggtt ccaggagcgc ggggcgcaga cttgtgccta atgcatgcgc gtgcagagcg
     1261 cccttgcagc acgctgccgc gcccggtttc ctcgaataag cgtggtcgtc ggcgcgcgca
     1321 ggagggtgag ccggtggcac ccgcctgcca cggcgcttcc tgccttgacc cagctggcat
     1381 gtgcatatcc cgccagccag cgccgagcga taccggtccc gctgatagcg acgtcgttcg
     1441 cgtgatcctc gtacccgagc cgtggcgccg atccgtgcgc tcgcgttggc gggggttgaa
     1501 gtcaaagatt tgtcggctgt gcacatgcaa cgtcgccatc agtcgcggtc gcccccctta
     1561 gtgcgggcaa aaatcagcga gctcgagacg cctcactttg ccaggcaatc gggtttctac
     1621 cgccttgtta ggcttcgaaa atcgctcggg caccggccaa gaccaaatcc gacatcttgg
     1681 cccaacaaga tcctctgccg gatcggtctt gcgagcgcgg tcgccactcc tggcgcatac
     1741 cgcgaaatca gaggatatcg ccaccgccgt cgcgccggca acctcattgt cgcgaagcct
     1801 ggccgcgctt tagttctcgg cggcagccgt gtgtcccaga gcatggaccc ccttcctgca
     1861 ccatgccata gtggcggcga tttttctggg cggggggtag cgcgatagca aatgtgccgg
     1921 ttgctcgcga tgctcgctct agccgtggtt atggtggggt taccggccct cgcctccacc
     1981 gtcctcgctg acaagcgcgt cgccctggtc atcggaaacg cggcctacga acatacgcca
     2041 aagctcaaca atgcaacgcg cgacgccgac gccatggccg cgcttttcaa gcgggctggg
     2101 ttcgaggtcg ttctcgaaca aacgaatgtc ggtaacctgg agttcaagcg ctcgctgcga
     2161 aatttcctac tggcggcgca ggacgcagac atcgcggtcc ttttttattc cgggcatg
//
LOCUS       DASTMT010000219         3849 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_4982902, whole genome shotgun sequence.
ACCESSION   DASTMT010000219 DASTMT010000000
VERSION     DASTMT010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3849)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3849)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3849
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4982902"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..272)
                     /locus_tag="VFR00_05160"
     CDS             complement(<1..272)
                     /locus_tag="VFR00_05160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0158674.1"
                     /translation="MAVAPPGARISWPRAGSLGRRGGWLASGLPRPSATRLGSAVLAV
                     AAALVAMAWGAADRATAFGRAGKEKLLPPAPKRASARPLLARAELQ"
     gene            420..641
                     /locus_tag="VFR00_05165"
     CDS             420..641
                     /locus_tag="VFR00_05165"
                     /inference="COORDINATES: protein motif:HMM:NF018294.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1127 domain-containing protein"
                     /protein_id="HEU0158675.1"
                     /translation="MSTTVGTAAIGPSAGANAILGAARRAWVAYMTWRIERIAINQLG
                     AMSDRQLKDIGLTRSEIAAAAKGALLFRR"
     gene            869..1969
                     /locus_tag="VFR00_05170"
     CDS             869..1969
                     /locus_tag="VFR00_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016843869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU0158676.1"
                     /translation="MASRRDFLQTTAAAITGLVFTDCHLRIAPPAAAQTRRREVVVNG
                     KRVKTVDIHAHCAVPEALALMNLKLGGPSLRPDLDTQGELAVRLKAMDEQGIDMEALS
                     INPNWYRLERDVAQQVIKLQNERLAEFCAKHPERFVAFASVALQFPDLAAEQLEEGVK
                     KFGLRGVGIGGNVAGAELSDPKFNPFWAKAEQLGVLVFIHPQGDGAPTQLQQRFKGNG
                     YLSNVIGNPLETTIALSHLIFEGTLDRYPGLKICAAHAGGYLPSYVGRFDQGCPTRPD
                     LCPGGTHGSIKKTPSEYLKGLYYDTMVFRPEGVRHVVAEVGASRLMVGTDFPYPWTTT
                     AIDVILDTPGLSDDDKIAILGGNAAKLLGLNA"
     gene            complement(2068..2760)
                     /locus_tag="VFR00_05175"
     CDS             complement(2068..2760)
                     /locus_tag="VFR00_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019196162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEU0158677.1"
                     /translation="MTSALVSKTVPPATPARPETLTEQAYNRLEEMIVTLSLAPGAVL
                     SEQTLSATLGIGRTPIREALQRLGHEGLVRVLPRKAIIVTDTDPKRQLLVLEVRRELE
                     RLLARASAERATEAERLRFRGIADGMEAAARTSDDIAFLRLDRDLNALLAAAAHNEYA
                     ARSMRFLNGHSRRFWYLHYKQAADLPKCARLHADEARAIAKANPARAMAASDKLIDYV
                     EAFTRTTVTGRR"
     gene            complement(2886..3185)
                     /locus_tag="VFR00_05180"
     CDS             complement(2886..3185)
                     /locus_tag="VFR00_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002713050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6166 domain-containing protein"
                     /protein_id="HEU0158678.1"
                     /translation="MRTFEGKRTIDGLLVTVDGRRLDEHYDVKCFTSWGFEWTYEGDS
                     PQQLALAILVEHLGDKARALRLCEPFMRKVIANLDNDWVLSAVEIEQAIRDIEAA"
     gene            3380..>3849
                     /locus_tag="VFR00_05185"
     CDS             3380..>3849
                     /locus_tag="VFR00_05185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0158679.1"
                     /translation="MSARARHSSYAHDDLFPGERIGRPLGGGGGKALLKAGLLLSLIG
                     GAAWSVLGDTGPLRQRFPHEAAALSRLIDRARRAFDEPGPATAALSSRRAADAKPASL
                     EPPPLGPVITARPLPAAPPPPAAEPAPLTTAALPSSPASPSPAGEPLEPPKVDPS"
BASE COUNT          645 a         1344 c         1256 g          604 t
ORIGIN      
        1 tcctgcagct cggcgcgggc aagcaagggg cgagcgctcg cccgcttcgg cgcgggcggc
       61 agcagttttt ccttgccggc acgcccgaag gcggtggcac gatcggcagc cccccaagcc
      121 atggcaacca aggccgctgc cacggccaag acggcagagc ccaggcgtgt ggccgagggc
      181 cgcggtaaac cgctggcgag ccagccgccg cgccggccca gcgagcccgc gcgcggccag
      241 ctgatgcggg cgccaggggg cgcgaccgcc aagcgtttcc tcgcgcgttt ccacgcccat
      301 tccaacgctg gctcccgtgc cggcgcacta aagactggag ccacggtgcc cagccgtgac
      361 gggtttcccg tgacaccggg cgcccccaat aacccagaca tgggaaacag gagatgatca
      421 tgagcacgac agtcggcacg gcagccatcg gcccatcggc cggagccaat gcgatcctgg
      481 gcgccgccag gcgcgcctgg gttgcctaca tgacctggcg catcgagcgc atcgccatca
      541 accaactcgg cgccatgagc gaccggcagc tcaaggatat cgggctcacg cgcagcgaga
      601 tcgcggccgc ggcgaaaggc gccctgctct tccgtcgcta gcgccacgcg aacggccgca
      661 cacgcggcgc aaggagcggg cagcggcggg cgtcgcgcgg ccctttgctg caattgcgtg
      721 ctcgctccct tcagcgagca tggcaatgtt gctatcgtgg cgcacaggca aagtgtgtgt
      781 gcgggcgaag gggttggcag cgtgcggtga agcgtccttg ccgacccgcg tgcagccacc
      841 catgcgacaa cggaaaggag cccgcatcat ggccagccgc agggactttt tgcaaacgac
      901 ggcagcagcg atcaccggcc tcgtctttac cgattgccac ctcaggatcg cgccaccggc
      961 ggcggcccag acacgccgac gcgaggtcgt cgtcaatggc aagcgcgtca agaccgtgga
     1021 catccatgcg cattgcgcgg tgcccgaagc gctggccttg atgaacctga agctcggggg
     1081 accttccctg cgccccgatc tcgacacgca aggcgagctc gccgtgcgcc tcaaggcgat
     1141 ggacgagcaa ggcatcgaca tggaggcgct cagcatcaat cccaactggt accggctcga
     1201 gcgcgacgtc gcccagcagg tgattaagct gcaaaatgaa cggctcgccg aattctgcgc
     1261 caaacatccc gaacgcttcg tcgcctttgc cagcgttgcc ctacagttcc ccgatctggc
     1321 cgccgagcag ctcgaggagg gcgtcaagaa gtttggcctg cgcggggtcg gcatcggcgg
     1381 caacgtcgcc ggcgcggagc tgagcgaccc caagttcaat ccgttttggg ccaaagccga
     1441 acagctgggc gtcctcgtct ttattcatcc gcagggcgac ggcgcaccca cgcaactgca
     1501 gcagcgcttc aagggcaatg gctatctcag caacgtgatc ggcaatccct tggagacgac
     1561 cattgcgctg tcgcacctta ttttcgaggg tacgctcgac cgctatcccg ggctcaagat
     1621 ctgcgccgcg cacgccggcg gctatctgcc ctcctacgtc gggcgcttcg atcaaggctg
     1681 tccgacgcgg ccagacctgt gcccgggtgg cacccacggc agcatcaaaa agaccccatc
     1741 cgagtatctg aagggcctct attacgacac catggtgttt cgtcccgaag gcgttcgcca
     1801 cgtggtggca gaagttggcg ccagccggct gatggtggga acggatttcc cctatccgtg
     1861 gacgactacg gcgatcgacg tcattctcga tacgccgggc ctcagcgacg acgacaagat
     1921 cgcaatcctc ggcggcaacg ccgccaaatt gctgggcctc aacgcttgat gcggtgatcc
     1981 acccccaaag ccgcgccgcg gcgtgggtgg cttggcgtta tcggcagtgg cacgcggcgg
     2041 ggtgcgctgc agcaaacccg gcgccggcta gcggcgcccg gtcacggtcg tgcgcgtaaa
     2101 ggcctcgaca tagtcgatca gcttgtcgga ggccgccatg gcgcgggccg ggttggcttt
     2161 ggcaatggcg cgggcttcat cggcgtgcag ccgcgcgcac ttcggcagat ccgccgcctg
     2221 cttatagtgc aggtaccaga accgccgcga gtggccgttc agaaagcgca tggaacgcgc
     2281 ggcatattcg ttgtgggcgg cagcggccag gagcgcgttc aagtcgcgat ccaagcgcaa
     2341 gaatgcaata tcatcgctgg tgcgcgcggc cgcctccatg ccgtcggcaa tgccgcgaaa
     2401 gcgcagacgc tcggcctcgg tggcgcgctc ggcgctggca cgcgcgagca agcgctcgag
     2461 ctcccggcgg acttccaaca ccaggagctg ccgcttgggg tcggtgtccg taacgatgat
     2521 ggctttgcgg ggcagcaccc gcacgagccc ctcgtgcccc aagcgctgca aggcctcgcg
     2581 aatgggcgtg cgcccgatgc cgagggttgc cgacagcgtt tgttcggaga gcaccgcacc
     2641 cggcgccagg ctcaaggtga cgatcatctc ctcgagccgg ttataggcct gctcggtaag
     2701 cgtctcgggt cgcgccggcg tggccggcgg cacagtcttg ctgacgagcg cactggtcat
     2761 gactaggctc gtatctcacc tcgctggcgc gcgcagcggc ccctggcggc aacgctgccc
     2821 cgccctggtt gcgcggcggc agcgcgcctg gcgtgagccg gccgcggcac tcgtcgcacc
     2881 ggctgtcatg ccgcttcgat gtcccggatc gcctgctcga tctcgacggc ggacaacacc
     2941 cagtcgttgt cgagattggc gataaccttg cgcataaacg gttcgcacag acgcagcgcg
     3001 cgcgccttgt cgcccagatg ttcgacgagg atggcaagcg cgagctgctg cggactgtcc
     3061 ccctcatagg tccattcgaa cccccaggag gtaaagcatt tgacatcata gtgctcgtcg
     3121 agccgtcgac cgtcgacggt gacgaggaga ccatcgatgg ttcgcttgcc ttcaaacgtg
     3181 cgcacgggtc ccctccagat gaagacgcgg tgatatatca ccgctgtgcc tcgccgcgat
     3241 agcccggccc gcgccccgac ccggggccaa gcgggtttga gcgccggctt tttttgccag
     3301 cttgccctca tttcgccagg aatttgctgg cgcctataca accgcgggga acccagcacg
     3361 acacgatcag gggtcagcca tgtcagcccg cgcccgccat tcgtcttacg cgcacgatga
     3421 tctcttcccg ggtgagagga ttggccgacc gcttggcggc ggcggcggca aagccctgct
     3481 caaagccggt ctcctgctct cgctgatcgg tggcgccgcc tggagcgttc tgggcgacac
     3541 cgggcccttg cgccagcgct tcccccacga ggctgccgcg ctctcgcgct tgatcgatcg
     3601 cgctcggcgc gcgttcgacg agcccggccc ggccaccgcc gctttgagca gccggcgtgc
     3661 ggcggacgcc aagcccgcat cgctcgagcc gccacccttg ggccccgtga tcaccgccag
     3721 accgctgccg gcggcgccgc ccccgcccgc ggcggaaccg gcgccgctga ccaccgccgc
     3781 gctgccgtcc agcccggcct cgcccagccc agcgggtgag ccgctcgagc cgcccaaggt
     3841 cgatccctc
//
LOCUS       DASTMT010000220         1996 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_2740387, whole genome shotgun sequence.
ACCESSION   DASTMT010000220 DASTMT010000000
VERSION     DASTMT010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 1996)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1996)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1996
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2740387"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..237)
                     /locus_tag="VFR00_05190"
     CDS             complement(<1..237)
                     /locus_tag="VFR00_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003610276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEU0158680.1"
                     /translation="MTRQSPFHPAVARWFSRTFAGPTSAQAEAWPAIQAGRHVLIAAP
                     TGSGKTLAAFLAAIDDLVRQGLEGRLSDETQVVYV"
     gene            complement(478..1743)
                     /locus_tag="VFR00_05195"
     CDS             complement(478..1743)
                     /locus_tag="VFR00_05195"
                     /inference="COORDINATES: protein motif:HMM:NF019844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEU0158681.1"
                     /translation="MLKTILRVTWCICLVTSARAEHGLLSGQEIADLVAGASVEIDTP
                     IGNKFPVRYAPDGQLSGQAGQLAFYLGTSTDRGRWWISTDQLCHRWNRWFNSEPQCLR
                     LRKEGSRIRWWTADGSSGTAVITATRLETAALARPQKDGQASQTPSEPAGPALAAALP
                     APVPSEPAPAPTPAMEAAVLAAASPTTPQAPAPDSKAKLSSPAPAEPKRPADPTYMVA
                     NVERDDVLNVRSGPSTEFDVIAELPPGSRGVSIIGTCRAGWCPVQHLAASGWVNRTFL
                     ALDAPQGAMARAQHGALPEDASGAEPPGAGSSWRDPVDAPRTCLTPAARALLEHIEAK
                     FGPVRLISTCRPGALIAGTGRPSRHASGNAVDFEAGARKGEIVDWLIANHHEGGTMTY
                     ADMNHIHVDIGPHFVSIAGGQHWASWRRP"
BASE COUNT          331 a          644 c          693 g          328 t
ORIGIN      
        1 gacgtagacg acctgggtct cgtcgctcaa gcgcccctcg agcccttggc gcaccaggtc
       61 atcgatggcg gcgagaaagg ccgcaagcgt tttgccgctg ccggtgggag ccgcgatcag
      121 cacgtgccgg cccgcctgga tggctggcca ggcttccgcc tgggcggacg tcggcccagc
      181 aaaggttcgg ctgaaccagc gggcaaccgc cgggtggaat gggctctggc gggtcatgcg
      241 cccttcgctg aggcttgatc cggcactgtg ggacaaaacc gaatgtcccc ctgtcggcag
      301 aatatgggat aggcaggggg gctttcaacg cggctgtcac gacgcctgat ttaccgcggc
      361 ccggcttcgg gcggggttga aaagggctcg gcagaccttt ccagggcccg agcccacggg
      421 cttttaggcc ctgccgtgcg gttcacaaag gctgatcccg atcggtggcg ccgtacctca
      481 cggccggcgc caactcgccc aatgctgccc gccggcgatc gaaacgaaat ggggcccgat
      541 atcgacgtgg atgtggttca tgtcggcgta ggtcatagtg ccgccctcat ggtgattggc
      601 gatcaaccag tcgacgatct cgcccttacg cgcacccgcc tcgaaatcca cggcgttacc
      661 gctcgcgtgc cgcgacggtc gtcccgtacc ggcgatcagc gccccgggtc ggcacgtcga
      721 gatcagtcgc acgggtccga acttggcctc gatatgttcc agaagcgcac gcgccgccgg
      781 ggtcaagcag gtgcgcggtg catcgacggg atcacgccag ctgctgccgg cgccaggtgg
      841 ctccgcgccg ctcgcgtcct cgggcaaggc cccgtgctgt gcgcgcgcca tagcgccttg
      901 cggcgcgtca agagcaagga aggtgcgatt gacccaaccg ctcgcggcca gatgctgcac
      961 ggggcaccaa cccgctcggc aggtgccgat aatgctaacg ccccggctgc ccggcggaag
     1021 ttctgcgatg acatcgaact cagtcgaggg accgctgcgc acattgagaa catcgtcgcg
     1081 ctccacattg gcgaccatgt aggtcggatc ggcgggacgc ttcggctccg caggggctgg
     1141 tgaggacagc tttgccttgg agtcgggcgc cggcgcttgg ggcgtcgtcg gtgacgcggc
     1201 cgcaaggacg gccgcctcca tggccggtgt cggcgccggc gctggctcgc tggggactgg
     1261 cgctggcagt gcggccgcga gcgcaggacc ggctggttcg gacggggtct gcgatgcttg
     1321 gccgtctttt tgcgggcgcg ccagcgcggc ggtctcgagc cgcgtcgccg taatcacggc
     1381 ggtgccgctg ctgccatccg ccgtccacca gcgaattctg ctgccctcct tcctcagccg
     1441 caagcattgg ggctcggaat tgaaccaccg gttccatcgg tgacagagtt ggtccgtgct
     1501 gatccaccag cgcccccgat ccgtgctggt gccgagatag aacgccagtt gccctgcctg
     1561 gccggagagt tggccgtcgg gcgcatagcg gaccggaaat ttgttgccga tgggggtgtc
     1621 gatttcgacg ctggcacccg ccacgagatc ggcaatctcc tgcccgctca ggagaccgtg
     1681 ctcggcccgg gcgctggtga ctaggcaaat gcaccacgtg accctaagga tcgtcttgag
     1741 cattgggccc ccaaaaacct gctgctcgat caagcttcta gaacgacgtg cagaaacaac
     1801 ttgtgagcga tgagtgtggc agcaagacgg agacaagcgc gccgatctgg gccggagaga
     1861 aacgccctgg cctcggggtc gggcaggagg aaacgtctgc ctcgcaaaag aaaggcgacg
     1921 tgcgcgcggt cgggagcggg acccttcgag gccacggcga gtgcaacgac gccgaccggg
     1981 tgacaaccgc gagcgg
//
LOCUS       DASTMT010000221         3127 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_1104739, whole genome shotgun sequence.
ACCESSION   DASTMT010000221 DASTMT010000000
VERSION     DASTMT010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3127)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3127)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3127
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1104739"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..379)
                     /gene="rpiA"
                     /locus_tag="VFR00_05200"
     CDS             complement(<1..379)
                     /gene="rpiA"
                     /locus_tag="VFR00_05200"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019197766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase A"
                     /protein_id="HEU0158682.1"
                     /translation="MRLGLGTGSTAAKFVDLVGQRVKQEGLQLVCVPTSEATRIRAER
                     LGLTLSTLESTPFLDLTVDGADELDDDLRLIKGGGGALLREKIVATASERMVVIADHS
                     KRVGTLGKFPLPVEVVSFGLTATR"
     gene            complement(457..1899)
                     /locus_tag="VFR00_05205"
     CDS             complement(457..1899)
                     /locus_tag="VFR00_05205"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013502160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEU0158683.1"
                     /translation="MDKPTISLIRNKCFVDGQWIGEGKLAVTNKATGDVITRVPEFGE
                     SETRGAIAAAHSALPAWSKLIAKERSKLIRAWYDEIIEHADELALLLSSEQGKPLAEA
                     KGEILYAAGFIEFFAEEAKRVYGETIPTFKADARIVVIKQPVGVVAAITPWNFPAAMI
                     TRKAGPALAVGCTMVLKPASETPLTALALAALAEKAGLPKGVFNVITGKASVIGKELT
                     GNALIRMLTFTGSTEVGRTLMAQCAPTIKKLGLELGGNAPFIVFDDADLDKAVAGAMA
                     SKYRNAGQTCVCANRIYVQDKVYDGFAQKLVAEVKKMRVGAGTEEGVTTGPLINKAAV
                     DKVEEHISDALGKGAKVVIGGKRHARGGNFYEPTVLANVTQDMLVAREETFGPVAPLF
                     RFKTEADVIAMANDTPFGLAAYFYARDVGRVWRIAEAIECGIIGINEGIISNEVAPFG
                     GYKQSGLGREGSHHGIEEYLEMKYMLMGGL"
     gene            2100..2783
                     /gene="gph"
                     /locus_tag="VFR00_05210"
     CDS             2100..2783
                     /gene="gph"
                     /locus_tag="VFR00_05210"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011927544.1"
                     /note="PGP is an essential enzyme in the glycolate salvage
                     pathway in higher organisms (photorespiration in plants).
                     Phosphoglycolate results from the oxidase activity of
                     RubisCO in the Calvin cycle when concentrations of carbon
                     dioxide are low relative to oxygen. This enzyme is a
                     member of the Haloacid Dehalogenase (HAD) superfamily of
                     aspartate-nucleophile hydrolase enzymes (PF00702); Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0009853 - photorespiration [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HEU0158684.1"
                     /translation="MQLADLTIVFDLDGTLVDTAPDLAAATNHALASAGLKPLTVAQL
                     RPLIGHGSRVMLEAGLRHHGLTLQEPELGALHERFFAYYADHVAVSSQPYAGVRELLE
                     ALRRAGTRLAVCTNKYEHLSRALLRQLDLAPLFASIAGRDTFPVCKPDPGHLTGTIER
                     AGGRVDAAVMVGDSEIDIATAKAAKVPSIAVTFGYTPRPVHEFAPDATIDDYAEFMAA
                     LARVRPPVA"
     gene            complement(2818..>3127)
                     /locus_tag="VFR00_05215"
     CDS             complement(2818..>3127)
                     /locus_tag="VFR00_05215"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HEU0158685.1"
                     /translation="TEIGVTNQRFILKRGLVWRSTHELQLRAIEEVNLEQDLLGRVFN
                     CGRLRLHGTGVDDIRLPVLADPIGLRKALQDGMAAANQASLAGASVAAAPVAPSRASL
                     "
BASE COUNT          516 a         1062 c          954 g          595 t
ORIGIN      
        1 ggcgggtcgc cgtcaatccg aaggacacca cctccacggg caagggaaat ttgcccagcg
       61 tgccgacgcg cttggagtgg tcggcgatca ccaccatgcg ctcggaggcc gtggcaacga
      121 tcttctcccg caacaaagcc ccgccgccac ccttgatgag ccttagatca tcgtcgagct
      181 catcggctcc atcgacggtg agatcgagaa agggtgtgct ctcgagcgtg ctgagcgtca
      241 ggccaaggcg ctcggcgcgg atcctggttg cctccgaggt cggcacgcac acgagttgca
      301 gaccctcttg cttgacgcgt tggcccacga gatcgacgaa cttcgccgcc gtcgagcccg
      361 tgcccagacc cagtcgcatg ccactcttca ccagagccag cgcctgttcc gccgccagct
      421 gtttgaagct gtcggacgaa tccggttcgg ccctcctcac aggccaccca tcagcatgta
      481 cttcatctcc agatactctt cgatgccatg gtgtgatccc tcgcgcccaa gaccactctg
      541 cttatagcct ccgaaggggg ccacctcatt ggaaatgata ccctcgttga tgccgatgat
      601 gccgcactcg atcgcctcgg cgatgcgcca gacgcgcccc acatcgcggg cataaaagta
      661 ggcggcgagc ccgaatggcg tgtcgttggc catggcgatg acatcggcct cggttttgaa
      721 gcggaacaac ggcgccacgg ggccgaaggt ctcctcgcgc gcaaccagca tgtcttgcgt
      781 cacgttggcg agcaccgtcg gttcgtagaa gttgccgcca cgcgcgtgcc gcttgccgcc
      841 aatcacgacc ttggcgcctt tgccgagggc gtcggaaatg tgctcttcca ccttgtccac
      901 ggcggccttg ttgataagtg gccccgtcgt cacgccctct tccgtgccgg cgccgacgcg
      961 catcttcttc acctcggcaa cgagcttctg ggcgaagccg tcatacacct tgtcttgcac
     1021 atagatgcgg ttggcgcaga cgcaggtttg tccggcgttg cgatacttcg aggccatcgc
     1081 cccggcgacg gccttgtcga ggtcggcatc gtcgaagacg atgaacggcg cattgccgcc
     1141 gagctcgagg cccagcttct tgatggttgg ggcacactgc gccataagcg tgcggccgac
     1201 ctcggtggac ccggtgaagg tcagcatgcg tatcagcgca tttcccgtca gctctttgcc
     1261 gatcacgctg gccttgccag tgatgacgtt gaacacgccc ttcggcaggc cggccttctc
     1321 ggccaaggcg gcaagcgcca gcgccgtcag cggcgtttcg gaggcgggtt tcagcaccat
     1381 cgtgcaaccc actgccagtg ccggtccggc cttgcgcgta atcatcgcgg cggggaagtt
     1441 ccagggtgtg atcgccgcca cgacgccgac gggctgcttg atgacgacga tgcgcgcgtc
     1501 cgccttgaag gtgggaatgg tctcaccgta gacgcgtttg gcctcctcgg cgaagaattc
     1561 aatgaagccc gcggcataga gaatttcgcc cttggcttcg gccagcggct tgccctgctc
     1621 gctgctgagc aacagcgcca attcgtcggc gtgctcaata atctcgtcat accaagcgcg
     1681 gataagcttc gagcgctcct tggcgatcag cttggaccag gcgggcaaag ccgaatgtgc
     1741 ggctgcaatc gcgccgcgcg tctcgctctc cccgaactcc ggcacccgcg tgatcacatc
     1801 ccccgtcgcc ttgttcgtga ccgctagctt gccttccccg atccactgcc cgtcaacaaa
     1861 gcacttgttg cgaatgagac tgatggtggg tttgtccatg gaacgcacct caattgtcgt
     1921 ccggccgcaa attgtcgccc ggccgcaccg ccgaacggtc cgcgccagtt gtagcatcat
     1981 ggtgtaggcc tgcatcgctc cccgtgcaag cgtacgttgc cacgccttgg cttggtgaca
     2041 ggcagcccaa agcccaatag cgtgcggcgt tctgcagctt ttttgcgcac tcgggacata
     2101 tgcagcttgc ggatctaacc atcgttttcg atctcgacgg tacgctcgtc gacacggcac
     2161 ccgatctcgc ggcggcgacc aatcacgcgc tagcctcggc cgggctcaag cctctgacgg
     2221 tggcgcagct gcgcccgctg atcgggcatg gctcgcgcgt catgctcgag gccggcctgc
     2281 gccatcacgg cttaacgctg caggaacccg agctcggcgc gttgcacgag cgcttctttg
     2341 cctattacgc cgatcacgtc gccgtgagca gccaacccta cgccggtgtg cgggagctgc
     2401 tcgaggccct gcgccgggcc ggcacccgcc tcgccgtctg caccaacaag tatgaacatc
     2461 tgtcgagggc gctgctgcgt cagctcgacc ttgcccctct gtttgccagt atcgccgggc
     2521 gcgacacctt ccccgtgtgc aagcccgacc ctggccattt gacggggacc atcgagcgcg
     2581 cgggcggccg cgtcgacgct gccgtgatgg tgggggatag tgaaatcgat atcgccaccg
     2641 ccaaagccgc caaggttccc tccatcgccg tcaccttcgg ttatacgccg cgtccggtgc
     2701 acgaatttgc gccggatgcc acgatcgacg actatgccga gttcatggcc gcgctggcgc
     2761 gcgtgcggcc gccggtcgct tgagcgggcc ctttccggaa acttgcccga agctcgttca
     2821 aaggctcgcc cgtgacggtg cgacgggcgc ggcggccacg ctggcgccgg cgaggctcgc
     2881 ctggttggcg gcggccatgc cgtcctgcaa agccttgcgc aggccgatgg ggtcggcgag
     2941 caccggcagg cgtatatcgt cgacccccgt gccgtgcagg cgcagccggc cgcaattgaa
     3001 gacccgcccc agcaggtcct gctccaaatt cacctcttcg atggcgcgca gttgcaactc
     3061 gtgcgtcgag cgccagacga gcccgcgctt gagaatgaac ctctggttgg tgacgccgat
     3121 ctcggtg
//
LOCUS       DASTMT010000222         3621 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_777042, whole genome shotgun sequence.
ACCESSION   DASTMT010000222 DASTMT010000000
VERSION     DASTMT010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3621)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3621
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_777042"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(148..765)
                     /gene="rpsD"
                     /locus_tag="VFR00_05220"
     CDS             complement(148..765)
                     /gene="rpsD"
                     /locus_tag="VFR00_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011088452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEU0158686.1"
                     /translation="MTKRIQAKHKIDRRLGQNIWGRPKSPINKRESRPGQHGERRSGK
                     LSDYGQQLRAKQKLKGYYANITERQFNGIYQAARRFKGSTSEHLIGLLERRLDAIVYR
                     AKFVPTMFAARQFVSHGHVTVNGRRVNIASYRCRVGDLIEIKEKAKQMTLLLEAIKSA
                     ERDVPDYIEADHNKMTAKLMRIPGLADVPFPVHMEPNLVVEFYSR"
     gene            complement(983..1621)
                     /locus_tag="VFR00_05225"
     CDS             complement(983..1621)
                     /locus_tag="VFR00_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0158687.1"
                     /translation="MIGNGARAGIGAVLALAVACGPASAQNKELSDKSVLTLMQYAWS
                     LVPNKFTSPDGKVIEVDKSKPSEVVVPMETAREAIKVARLSAYAQVCDLIEEQRANYH
                     TFMKREEAKGKWSDQQLLFINQLHLFTVMTLTGKVKLVEKDGDKEVILNSDKPEKAPT
                     CTDTEKKKVESQIKSYVAANVPSTPAPAAPAAAAAPAPPAAAPQVKPASQKK"
     gene            complement(1733..2971)
                     /locus_tag="VFR00_05230"
     CDS             complement(1733..2971)
                     /locus_tag="VFR00_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEU0158688.1"
                     /translation="MKGIVVSAGEPRIAGTELAVGAVVERCYQTSIDTGLGEMAFAGL
                     DRAALEAVLTYCAEQRCRKDDVSCPGCRLRTERLGLKSFDDFVARFAEVGFSHSTVRL
                     IGGGSDRLEAESLAKLAKTWAGEEYWFWARRLLRKLRHGLRRANLSGAPPEPRAAPVL
                     VLVRPQLADNIGMVARAMANFGLEELRLVAPRDGWPNEKARIAASGANFVIDGAEAYP
                     SLAGALTGLHWVAATSARQRDLAKPVMTPEGAIAEIADRLAAGQRCGIVFGPERNGLE
                     TEEIANADALIMAPVNPAFASLNLAQAALLMSYEWMKRAGHGTLGRVTTYEQPVAAGL
                     RLRGSLPAGREELMGFFEHLERELDARGFFSSPEKRPSMVRNMRTMFTRMGATQQEIR
                     TLRGIVKALVHGRRTTRHSP"
BASE COUNT          577 a         1189 c         1193 g          662 t
ORIGIN      
        1 cgtcgatgcc gagcgccagt cggcagaacg ggccagcaac ccaccgagcg gtccgcgtcc
       61 gggcacgaag ccataacggc gagagcataa aatgcgcggc cggcgagccc tctcgccggc
      121 cgttttgttt gccggctcca atccgcgtca gcggctgtag aactccacca ccaggttggg
      181 ttccatgtgc acgggaaagg gcacatcggc gagaccggga atgcgcatga gcttggcggt
      241 catcttgttg tggtcggcct cgatgtagtc ggggacatcg cgttccgcac tcttgatggc
      301 ctcgagcagg agcgtcatct gcttggcttt ttccttgatc tcgatcaggt cgccgacgcg
      361 gcagcgatag ctcgcgatat tgacccgccg gccattcacc gtgacgtgac catggctcac
      421 gaattggcgg gcggcgaaca tggtgggtac gaatttggcg cggtagacaa tggcgtcaag
      481 ccgccgttcc agtaggccta tcaggtgttc ggaggtggaa cccttgaagc ggcgcgccgc
      541 ctgatagatg ccgttgaatt gccgctcggt gatgttggcg tagtagcctt tcagcttctg
      601 tttggctctg agctgttggc cgtagtcgga cagcttgccg ctgcgccgct cgccgtgctg
      661 gcccggccgg ctttcgcgct tgttgatcgg gctttttggc cgcccccaga tgttttggcc
      721 caggcgccga tcgatcttgt gcttggcctg gatacgcttg gtcatgcttc aggtctccaa
      781 agtcgcgcag aaattgtgag ctcgaatagc acgcacgccc tcctctaccc ggctgcagcc
      841 gaatcgacag gtacccgcca gggcgggcca ccgcggatgt gcaaacgcga agggcgggcc
      901 aaacggcccg ccggtcccgc cttatatagc aagggtgtgc caggtcaatg cggcaaccga
      961 gccgcggccg ctggcctcgg ccttatttct tctggctggc gggtttgacc tggggggccg
     1021 ccgccggcgg ggcgggtgcc gccgcggccg ctggcgcggc cggcgctggc gtcgagggca
     1081 cgttggcggc gacgtaggat ttgatctggc tctcgacctt ctttttttcc gtgtcggtac
     1141 aggtcggcgc cttttccggc ttgtcgctgt tgaggatgac ctctttgtcc ccatccttct
     1201 caaccagttt gaccttgccc gtcagcgtca tgacggtgaa caggtgcagc tgattgatga
     1261 acagaagctg ctggtcgctc cactttccct tggcttcctc gcgcttcatg aaggtgtggt
     1321 agttggcccg ctgctcctcg atcaggtcgc agacctgcgc ataggccgac aggcgtgcca
     1381 ccttgatggc ctcgcgcgcc gtctccatgg ggacgaccac ctcgctgggc ttgcttttgt
     1441 cgacctcgat caccttgccg tccgggctgg taaacttgtt tggcaccagc gaccaggcgt
     1501 actgcatcag ggtcaggacc gatttgtcgc tgagctcctt gttttgtgcc gaggccggcc
     1561 cgcacgctac cgccagcgcc aacaccgccc caatgccggc ccttgcaccg ttgccgatca
     1621 ttccgcagcc cctgtctgtc cagcccaatt gcaacgttct tgtctcgttc gccaacggca
     1681 gtcccactct gcctagcgcg tgcgcatcag ctcgacggga atcgaggcat aactaaggcg
     1741 aatgccgcgt cgtccgccta ccgtgtacaa gcgccttgac gattccccgc agggtgcgga
     1801 tttcctgctg cgtcgctccc atgcgcgtaa acatggtgcg catatttcgc accatgctgg
     1861 ggcgcttttc gggcgatgag aaaaagcccc gggcgtccag ctcacgctcg agatgctcga
     1921 agaagcccat cagttcctcg cggccggcgg gcagcgagcc gcgcaggcgc aggccggcgg
     1981 ccaccggctg ctcgtaggtg gttacgcgcc ccaaggtgcc gtggccggcc cgtttcatcc
     2041 attcataact catcagcaag gcggcctgcg cgaggttgag agaggcaaag gccggattga
     2101 cgggggccat gatcagggca tcggcattgg cgatttcctc cgtttcaaga ccattgcgct
     2161 ccggcccaaa gacaatgccg cagcgctgcc cggcggcaag gcggtcagca atttcggcga
     2221 tggccccttc cggggtcatg accggcttgg ccaggtcgcg ctgccgagcc gaggtggccg
     2281 cgacccagtg gaggcccgtc agcgcgccgg ccagggaggg ataggcttcg gcaccatcga
     2341 tcacgaagtt ggcgcccgag gcggcaatgc gcgccttctc gttgggccag ccatcgcgcg
     2401 gggcaacgag gcgcaactcc tcgaggccga agttggccat ggcgcgtgcc accatgccaa
     2461 tgttgtcggc gagctgcggt cgcaccagga ccaggaccgg cgcggcgcga ggctcgggcg
     2521 gggcaccgct caggttggcg cggcgtaggc cgtgacgcag cttgcgcagc agccgccggg
     2581 cccaaaacca gtactcctcc ccggcccagg tctttgccag tttggccagg ctttcggcct
     2641 cgagcctatc gctgccgccg ccaatgaggc gaacggttga gtggctgaag ccgacctcgg
     2701 cgaagcgagc aacgaaatca tcgaagctct tgagaccgag ccgttccgtg cgcaaccggc
     2761 agcccggaca gctcacatcg tccttgcgac agcgctgctc ggcgcaatag gtgagcacgg
     2821 cctcaagggc cgcgcggtcg agccccgcaa acgccatctc gccgagccca gtatcgatgc
     2881 tcgtctgata gcagcgctcg accacggcgc cgaccgcgag ctcggttccg gcaatgcgcg
     2941 gttcgcccgc gctgacgacg atgcctttca cctcacctgc acctctgacg ctccttcgat
     3001 gcgcaatacc ttgcccgtca cccaccgggc acaagcgccc attgtagcgg gcgggggcgc
     3061 atcgtgcaca tgtgcggcgc ctggcgagcg cttgagcagc gcaaaaagtc gacgatccgc
     3121 cgcgtctccg cgtgcctcgg cctcaggctg gcgcagcgcg ccgatgtagg cgatggtggt
     3181 cgcgtcggtg gccgaccgcg cggccccgcc ggtccggcgc cacggcctga aagcgctcgg
     3241 ctgagcccgg cagcagctcc acccaggccc cccgcgtagt cgggaaagcc gtgcaggaac
     3301 aggaggagtg gggcgccggc ctcccccaag acccgcaggc ttaggtcgat gccgctgacg
     3361 tggaccgagg gggtgcgcat ggcacaccct cggcggcccg ctggcgcggc agttgcgcct
     3421 gccgccgacg ccggccaaaa gccgtgacgg tgtcgagagt tggtggcctc ctgcggcgcc
     3481 agcaaccttt gcgcggggcc gcaccggtgc taagagggcg gcgcgcgacg cgattggagg
     3541 gagcttccat gcagaagatc aaggtcaccg gcaccgtcgt cgagatggac ggcgatgaga
     3601 tgacgcggat catctggcag c
//
LOCUS       DASTMT010000223         4452 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_6297185, whole genome shotgun sequence.
ACCESSION   DASTMT010000223 DASTMT010000000
VERSION     DASTMT010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4452)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4452)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4452
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6297185"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1455)
                     /locus_tag="VFR00_05235"
     CDS             complement(<1..1455)
                     /locus_tag="VFR00_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015665350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEU0158689.1"
                     /translation="MTELSTHRKITISNVSRRDLLKGVVASGGLVLAAQLTGVKGAFA
                     GYPTGASAMPNGVVSDPKIFVSIGGDGIVSIVAARAEMGTGAARTALPMMVADEMGAD
                     WARVKVVQSPGDEKTYGNQDTDGSRSVRHFIQPMRQCGAAARQMLEAAAAKKWGVDVG
                     EVETQVHEVVHKPSGRKLGFGELAADAASQPVPAEDKIKLKDASAFRYIGKGNVRPTD
                     QVDITTGHAVYGQDVMLPGMKFAVVARPPVVGGKVASLDSSAAMKVPGVEKIVTLAPT
                     PAPYKFAPLGGVAVIAKSTWAALKGRDALKITWDAGPNKAYDSKAYRAQLEESVRKPG
                     KVERNVGDVDKALASAAKVITAEYYAPHMHHATMEPPAAAASMSNGKWEVWAPVQSPG
                     QARDDIAKALGVKPEELVLHTTLLGGGFGRKSKCDFAIEAALLSKELGGTPVKVVWTR
                     EDDLQHGFYHTVTAERFEAGVDANNKVIAWRHRSA"
     gene            complement(1457..1909)
                     /locus_tag="VFR00_05240"
     CDS             complement(1457..1909)
                     /locus_tag="VFR00_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010684967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEU0158690.1"
                     /translation="MANLRVNGQERTYDGDPSMPLLWYLRDELALTGTKFGCGVGLCG
                     ACTVHIDGAAQRACMVQMSEATGKAVTTIEGLHPKHEHPVQVAWREVSVPQCGYCQAG
                     QIMQAAALLAKKPAPTDAEITSGMTGNLCRCGCYQRIHAAVRAAAKGA"
     gene            2540..3733
                     /locus_tag="VFR00_05245"
     CDS             2540..3733
                     /locus_tag="VFR00_05245"
                     /inference="COORDINATES: protein motif:HMM:NF021508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2235 domain-containing protein"
                     /protein_id="HEU0158691.1"
                     /translation="MANLVLCLDGTWNNADSAAPQSNVAILSDLVDLRRGGKSAQQLY
                     YDPGLGTAGNFLERLAAGVGGQGLSANVLGAYRFLSLSYRPGDNIYLFGYSRGAFSAR
                     SLCGFIAASGLLRAELCDKANLDFAWSYYRTRPKDRFPSDHAKLREMSYADVRIRFVG
                     VFDTVGSLGIPRKWLNWIGRRSFQFHDTELCSIVDHSCQALAIDEHRMEFEAAVWDVP
                     RRGGYHTVEQVWFPGVHANIGGGCEDKGLSDLTLDWMLKRVRRYCPELALRAVRLEPD
                     HRAMLYEPRSWLYWRSIRWPLIRIINRCPVKRGSGRFRLARVRPHSQSIGEMLHWSAL
                     LRYLETRKLPPKKRYAPTNLLAALESAKRGATLIVGPDGEPGPYFQPSPRRPADAPAA
                     RLEYH"
     gene            complement(3778..4287)
                     /locus_tag="VFR00_05250"
     CDS             complement(3778..4287)
                     /locus_tag="VFR00_05250"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEU0158692.1"
                     /translation="MRFLAFIGVIAILAALLVGGYFFGGFFDVGAGWEDPAPVVSALT
                     RVRAASIERRAHAKAPLPLDDAASISAGARQYAKYGCTHCHGAPGAEWSKFSEGLNPG
                     PPDLKDVVKERSPAQLFWVVKNGIRMTGMPSFGKAGVPDPEIWQIVAFLQKLPSVSEA
                     DYKSWTAAQ"
BASE COUNT          704 a         1487 c         1472 g          789 t
ORIGIN      
        1 ggcgctgcga tgccgccagg cgatcacctt gttgttggcg tcgacgcccg cctcgaagcg
       61 ctctgcggtc acggtgtgat agaagccgtg ctgcagatcg tcctcgcgtg tccacaccac
      121 cttgaccggc gtgccaccca attctttgga gagcagcgcc gcctcgatgg cgaagtcaca
      181 cttggatttg cgcccgaaac cgccgccgag cagcgtggtg tgcagcacca actcctcggg
      241 tttgacgcca agcgccttgg caatatcgtc gcgtgcctgg ccggggcttt gcaccggcgc
      301 ccacacttcc cacttgccat tgctcatgct ggccgcggcc gccggaggct ccatggtcgc
      361 atgatgcatg tggggagcat agtattcggc cgtgatcacc ttggccgcgg acgccagcgc
      421 cttgtccacg tcgccgacgt tgcgttcgac cttgccgggc ttgcgtacgc tctcttcgag
      481 ttgggcccga taggccttgg agtcgtaggc cttgtttggc cccgcatccc aggtgatctt
      541 gagcgcgtcg cgacccttga gcgccgccca ggtgctcttg gcaatgacgg ccacgccgcc
      601 caagggggcg aatttgtagg gcgcgggcgt cggcgcaagc gtgacgattt tctcgacgcc
      661 aggaaccttc atggcggcgc tgctgtcgag agaggcgacc ttgccgccaa cgacgggcgg
      721 cctggcgacg acggcgaact tcatgcccgg cagcatgacg tcctggccgt agacggcgtg
      781 cccggtcgtg atgtcgacct ggtccgtcgg acgcacgttg cctttgccga tatagcggaa
      841 ggcgctggcg tccttgagct tgatcttgtc ctccgccggc actggctggg acgcggcatc
      901 ggcggcaagc tcaccgaagc cgagcttgcg gcccgagggc ttgtgcacca cttcgtgcac
      961 ctgcgtctcg acctcgccga catcgacgcc ccacttcttg gcggccgccg cctcgagcat
     1021 ctgccgtgcg gcagcgccgc attggcgcat cggctggatg aagtggcgta cgctgcgcga
     1081 accgtcggtg tcctggttgc cgtaggtctt ctcgtcaccg ggcgactgca cgaccttgac
     1141 gcgcgcccag tcggcgccca tttcgtcggc caccatcatc ggcagcgccg tgcgggcggc
     1201 accagtgccc atttcggcgc gggccgcgac gatgctgaca atgccgtcgc cgccgatcga
     1261 cacgaagatc ttcgggtcgg aaacgacgcc gttcggcatg gcggaggcgc cggtcggata
     1321 gccggcgaag gcgcctttga cgcccgtcag ttgcgcggcg agcacgagcc cgcccgaggc
     1381 aaccacgccc ttcaagaggt cgcggcggct aacgttgctg atggtaattt tgcgatgcgt
     1441 gctgagctct gtcatgtcac gcccccttgg cagcggcgcg cacggccgcg tggatgcgtt
     1501 gatagcaacc gcagcgacag agattgccgg tcatcccgga ggtgatctcc gcatcggtcg
     1561 gcgccggctt tttggccaac agcgcggccg cttgcatgat ctggccggcc tggcaatacc
     1621 cgcactgggg cacgctcacc tcgcgccagg caacctgcac ggggtgctcg tgcttggggt
     1681 ggaggccttc gatggtggtg acggccttgc cggtcgcctc tgacatctgc accatgcagg
     1741 cacgctgagc cgcgccgtcg atgtgcacgg tgcaggcgcc gcacaggccg accccgcacc
     1801 caaacttggt gccggtcagc gccagctcgt cgcgcagata ccacaggagc ggcatcgacg
     1861 gatcaccgtc gtaggtgcgc tcctgcccat ttacccgtaa gttcgccatt gatcctacct
     1921 ccttggccgc aattcccgaa cgcggggcgc tcatgccttc gcgccgccaa cccggcagaa
     1981 cgaagcagca gaccctctta tttcgtggcc ggcagctgcc ggctgtgttg accggtcaaa
     2041 tctggccgac actgtcaccc ctgccacagc aagggcgctg ctggccgtgg tgacgaggca
     2101 atgaaaaaag gtcctaaacc ggggtccgct gggtggtcac tgtacttcgg atttcaaggg
     2161 gtcgttgtgg cgggatcgat cacaagcgcg tgacgtgtga ggcgcagtcc cgttgggagc
     2221 aggcccacaa ggccgaaagc caaccgccgc gccagtcagc aacgcgggcg agcgcggttg
     2281 accaggcgct gccgccgtgg gccggcctgg gcgagccccg gcggcttcaa gagttcgcgg
     2341 cgtgtgggca atcgccaagc cagcggcatg cccgggccgg cgccggccag gtcagccctg
     2401 ggccaccgcc gctcggcggc cttgtccgcg gttgcgcaca aaaaagcgaa ggcacggccg
     2461 cgctcaaggc cgcgggccag cgcaacgcag ccaaagaatc agtgcacact tccccgcctt
     2521 ttggcttgag ggagcaagca tggccaacct ggtgctttgc ctggatggca cgtggaacaa
     2581 tgccgatagc gccgcgccac aaagcaacgt ggcgatcctg tccgatctcg tcgatctgcg
     2641 ccggggcggc aagtcggccc agcagctcta ctatgatccg ggcctcggca ccgcgggcaa
     2701 tttcctggaa cgtctcgcgg ccggcgtcgg cggccagggt ctcagtgcca acgtgctcgg
     2761 cgcctaccgc ttcctctccc tcagctaccg ccccggcgac aacatctacc tcttcggcta
     2821 ctcgcgcgga gcgtttagcg cgcgcagcct gtgcggcttt atcgccgcca gcggtctgtt
     2881 gcgggcggaa ttgtgcgaca aggcgaacct cgattttgcc tggagctact accgcacgcg
     2941 accgaaggac cggtttccct ccgatcatgc caagctccgc gagatgagct atgccgacgt
     3001 gcggatccgc ttcgtgggcg tcttcgacac cgtcggctca cttggtattc cgcgcaaatg
     3061 gctgaattgg attggacggc gctcgtttca gtttcacgac accgaactgt gcagcatcgt
     3121 cgatcattcc tgccaggccc ttgccatcga cgagcatcgc atggagttcg aagccgcggt
     3181 gtgggacgtg ccccggcgcg ggggttacca caccgtcgag caggtgtggt ttcccggggt
     3241 gcatgccaac atcggcggcg gctgcgagga caaggggcta tccgatctga cgctcgactg
     3301 gatgctcaaa cgcgtgcggc gctattgccc cgaactggcg ctgcgcgccg tgcgcctcga
     3361 gcccgaccat cgggccatgc tctacgagcc gcgcagttgg ctctactggc gcagcatccg
     3421 ctggccgctc atccgcatta tcaaccgctg cccggtgaag cggggcagcg ggcgctttcg
     3481 actggcgcgc gtccgaccgc attcgcaaag catcggcgaa atgctgcatt ggagcgcgct
     3541 gctgcgctat ctggaaacgc gcaagctgcc gccgaaaaaa cgctatgcgc ccaccaatct
     3601 gttggcggcg ttggaaagcg ccaaacgggg tgcgacgctc atcgtcggcc ccgatgggga
     3661 acctgggccc tatttccaac cctcgcccag gcgtccggcc gatgctcccg cggcgcggct
     3721 ggaatatcat tagccgccgc gtggggtcgc cgctcgggcg cgcgcctcgg cgcgccttta
     3781 ttgcgcagcc gtccagctct tgtagtccgc ctccgacacg ctcggtagct tctgcaggaa
     3841 ggcaacgatt tgccagatct cggggtcggg cacgcccgcc ttgccgaagc tcggcatgcc
     3901 ggtcatgcgg atcccgtttt tgaccaccca gaagagctgc gccggtgagc gctccttgac
     3961 gacgtctttg agatcaggcg ggccggggtt cagcccttcg gagaacttcg accactccgc
     4021 accgggggca ccgtggcagt gcgtgcagcc gtacttggcg tattggcgcg cccccgcgct
     4081 gatgctggcg gcgtcgtcga gcggcagcgg cgccttggca tgggcgcggc gctcaatcga
     4141 ggccgcgcgc acgcgcgtca aggccgagac aacgggggcg gggtcttccc agccggcgcc
     4201 gacgtcgaag aagcccccga agaaatagcc gcccacgaga agggcggcga ggatcgcgat
     4261 tacgccgatg aacgctagaa aacgcatggc tggcctcgca acaggaaggg gcgccgtcga
     4321 gcgctaatgc cgcttgcctg attgaacgct caagcctggt ctttgatccg gccgcgtcga
     4381 gggggcaatc gtctagagag accggggttg aggcttgcgc gtcccgggtt gggcgcccga
     4441 ggcgcggcgc tt
//
LOCUS       DASTMT010000224         1611 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_5968710, whole genome shotgun sequence.
ACCESSION   DASTMT010000224 DASTMT010000000
VERSION     DASTMT010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 1611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1611
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5968710"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..275)
                     /locus_tag="VFR00_05255"
     CDS             complement(<1..275)
                     /locus_tag="VFR00_05255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0158693.1"
                     /translation="MPIAEADRKRIVQAIRSYIARERISRGEFAERAKLGKSTVDKLV
                     VGLFSEKTILQIESQLKISLVGGGGPGEAAAEDLGRYTKEETQNYVG"
     gene            393..686
                     /locus_tag="VFR00_05260"
     CDS             393..686
                     /locus_tag="VFR00_05260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0158694.1"
                     /translation="MRSLLPPAAVFAIVLWGLPTVAAAADRSGEQPFGGPPPQRQIEE
                     RPFGGPPPRSRSIQGDGFACRTATSVCRLEKAKPVGAACTCPGGPSGVPGKVE"
     gene            complement(873..>1611)
                     /locus_tag="VFR00_05265"
     CDS             complement(873..>1611)
                     /locus_tag="VFR00_05265"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018094145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HEU0158695.1"
                     /translation="MLLGLIFRGVAFEFRFRSRRRKFLWDWAFAGGSTLAAFAQGIAL
                     GALVQGVPVEGRAYAGGWWDWLTWFSLLTGVALLIGYGLLGATWLIWKTSGALQQKAF
                     SLAFSTGVGTLALIALVSLIMPFLDPIFRQRWFTWPNIAYAAPVPILVAISAFVLLSG
                     LAKRWERAPFLAAVALFVLSYIGLGISFYPYILPPSLTIWDAAAPASSLRFLLVGAVV
                     LIPLILSYTAWSYWVFRGKVSPDARYH"
BASE COUNT          320 a          516 c          540 g          235 t
ORIGIN      
        1 tcgcccacgt agttttgcgt ttcctccttg gtgtagcggc ccaaatcctc ggcggcggcc
       61 tcgcccggtc caccgccgcc caccaggctg atcttcagct gcgattcgat ctgcaggatg
      121 gttttttcgg aaaacaagcc gacgaccagc ttgtcgacgg tcgatttacc tagcttggcg
      181 cgctcggcga actcgccccg cgagatgcgt tccctggcga tgtagctgcg tatcgcctgc
      241 acgatacgct tgcgatcagc ttccgcgatt ggcatggcgc gcctattgaa ccgatcgggg
      301 cgtgcggcac aactatcatg gcgggctccg gtgcggtttg ctaccctgcg cacgccgggg
      361 atgttcgcga gtttgtgtcg agggagcatc gaatgaggtc gttactgccg ccagcggcgg
      421 tgtttgcgat cgtcctctgg ggcttgccca ccgttgcagc ggcggcggac cgcagtggcg
      481 agcagccgtt tgggggtcca cctccacaac ggcagatcga ggaaagaccc ttcggaggcc
      541 cgccgccgcg aagtcgctcc attcagggcg acggttttgc ctgccgcacc gcgaccagcg
      601 tctgccggct ggaaaaggcc aaacccgtcg gcgccgcctg cacctgcccg ggcggtccga
      661 gcggcgtgcc gggcaaggtc gaatagcgtc tgcgccttgc cggcccgggg cgcgccgcga
      721 gggcctatga ccggcgcgcg ggcccaacgt tgttaggaca gaaccgcgcg tagccccaag
      781 ccgagcaaga gcacgcacgc cacgcctgcc agccaaaggc cggcaaacca cacgagccgg
      841 cgcagcaacg agggtggggt gctggacgcc attcaatgat accgcgcatc cgggctgacc
      901 ttgccgcgga acacccaata cgaccaagcc gtgtagctca ggatcagggg gatgagaaca
      961 acggccccga ccaggaggaa gcggaggctg ctcgccggcg cggccgcatc ccagatggtc
     1021 agcgacggcg gcaggatgta gggataaaag ctgatgccga gcccgatata ggagaggacg
     1081 aagagggcga cggcagccag gaacggcgcg cgttcccagc gcttggccag accggagagg
     1141 agcacgaagg cgctaatcgc gacgaggatt ggcaccggtg cggcataggc aatgttgggc
     1201 caggtgaacc agcgctggcg gaagatggga tcgaggaatg gcatgatgag actgacgaga
     1261 gcgatcagcg ccagggtgcc aacccccgtc gagaaggcca gggaaaaagc cttttgctgc
     1321 agcgcgccgc tggttttcca gatcagccag gtcgcgccga gcagaccata gccgatgaga
     1381 agcgccacgc cggtcaagag gctgaaccag gtcagccaat cccaccaacc gcccgcgtag
     1441 gcgcggccct cgaccggaac accttggacc agagcgccca gcgcgatgcc ctgggcgaag
     1501 gccgccagcg tcgagccgcc ggcgaacgcc caatcccata gaaatttgcg cctcctgctg
     1561 cgaaaacgaa actcgaaggc aacgccgcgg aaaatcagcc cgagcagcat g
//
LOCUS       DASTMT010000225         2408 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_6297526, whole genome shotgun sequence.
ACCESSION   DASTMT010000225 DASTMT010000000
VERSION     DASTMT010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2408)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2408)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2408
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6297526"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..637
                     /locus_tag="VFR00_05270"
     CDS             <1..637
                     /locus_tag="VFR00_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008141104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0158696.1"
                     /translation="AVLEGQTTALLGRNGAGKSTTLTALAGLAAPRRGTIRIGGVAFQ
                     GRKPHAVARAGIGYVPEDRQIFPEHSVEDNLLIGTKKGPDGQDYWTLPRIYQMFPLLE
                     PLRNRLGGRLSGGEQQMLTIARTLMGNPIGLLLDEPSEGLAPVIVARIGELLRRLRDM
                     GVTVLLAEQNMHFCLGIASHAVVIDKGRIVYRNSIDALKSDDAVKKRYLAL"
     gene            698..1666
                     /locus_tag="VFR00_05275"
     CDS             698..1666
                     /locus_tag="VFR00_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007591774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU0158697.1"
                     /translation="MATASGLDPDLARILPLLPLRDAASLTPARARQDLVALADSRKD
                     VPLPQPAAVADTEIPTAAGALAARIYRPSRMPTGTLMYFHGGGWVAGDLYTHDRLART
                     LAIELEAVVLSVDYRRPPEAPFPCAFEDCFAATQWAHRHIDKLGGDAKRLGVGGDSAG
                     GNLAAAVALAWRNEAQGLAAQLLIYPVTDVVGCYGESSDNARYPSRGENAEGYFLTLE
                     AMRFFARAYLAEPHDAADPRVSPLRAAELHGLAPAVVCTAEFDPLRDEGEAYAKRLAS
                     AGIPVSYFREPGMIHGYFGMGGASPAADAARRRATDKFKQLLVGTA"
BASE COUNT          424 a          792 c          778 g          414 t
ORIGIN      
        1 tgccgtgctg gaaggccaga ccaccgcact cctaggtcgc aatggcgcag gcaagtccac
       61 cacgctcacg gcgctcgccg gcctggcggc gccgcgccgc ggaaccatcc gcatcggcgg
      121 cgtggcgttc caaggccgca aaccccatgc cgtggcgcgc gccggcatcg gctatgttcc
      181 cgaggaccgg caaatctttc cggagcactc cgtcgaggac aacctgctga tcggcacgaa
      241 gaagggcccg gacggacagg actattggac gctgccgcgc atctaccaga tgttcccgct
      301 gctcgagccg ctgcggaacc ggctgggcgg gcggctctcg ggcggcgagc agcagatgct
      361 gaccatcgcc cgcacgctga tgggtaatcc aatcgggctt ttgctggacg aaccgagtga
      421 agggctggcg cccgtcattg tcgcacgcat tggcgagctc ttgcgtcgcc tgcgcgacat
      481 gggggtcacc gtgctgttgg ccgaacagaa catgcatttc tgcctgggca tcgccagcca
      541 cgcggtggtc atcgacaagg gccggatcgt gtaccgtaac agcatcgacg cactgaaaag
      601 cgatgacgcc gtcaagaagc gctacttggc cttgtaggga ccggcagtat ggcaatggcc
      661 tcggggcagc gaggcagcaa gcagggagat cgtcaccatg gcaacggcaa gcggactaga
      721 ccccgatctt gcgaggatcc tgccgctgtt gcccctcagg gatgcggcga gcctgacgcc
      781 cgcccgcgcc cgccaggacc tggtggcact ggcggactcc cgcaaagatg tgccgctgcc
      841 ccagcccgct gccgttgcgg acacggaaat ccccaccgcc gccggcgcgc ttgcggcgcg
      901 catctaccgg ccgagccgca tgccgaccgg aacgttaatg tatttccatg gcgggggatg
      961 ggtcgccggc gatctctaca cacacgatcg gctggcccga acgctggcga tcgaactcga
     1021 ggccgtcgtg ctgagcgtgg actatcgccg tccgccggag gcacctttcc cctgcgcctt
     1081 cgaggactgc ttcgccgcca cgcaatgggc gcatcgccat atcgacaagc tcggcggtga
     1141 tgccaagagg ctgggcgtgg gcggcgacag cgccggaggc aacttggctg cggccgtggc
     1201 gcttgcctgg cgcaacgagg cgcaggggct cgcggcccaa ctcttgattt atccggtaac
     1261 cgacgtcgtc ggctgctacg gcgagagtag tgacaacgcc cgatacccct cgagggggga
     1321 gaacgcggag ggttattttc tgacgctcga ggcgatgcgt ttttttgcgc gtgcctacct
     1381 tgccgagccg catgacgccg ccgatccgcg ggtctcgccg ctgcgggccg ccgagctcca
     1441 cgggttggcg ccggccgtgg tttgcacggc ggaattcgat cccttgcggg acgaaggcga
     1501 ggcctacgcc aagcgcctgg cgtcggccgg tattcccgtc agctatttcc gcgagcccgg
     1561 catgatccat ggctacttcg gcatgggcgg agcctccccc gcagcagatg ccgcccgacg
     1621 ccgggccacc gacaaattca agcagctgct ggttggaacg gcctgaggca gacctgcgca
     1681 ctatcgccaa accgggtgct cgagatttgc ggcagcggct ttgagtgccg caaggccgcg
     1741 gtgcgcaacg caccggcagg cgccgaagcg cgaaaagacg ggattgtcag gggcgatcgg
     1801 ccgaaagagg gtggcgcggg ggttgctcgc tcgctcgcag tgacaaagtc gttcttagcc
     1861 gaggcaatgg gcgacgtcct cttcagccaa gctgcggacc aactcggagg cctcctcagt
     1921 cctctgcaat tcctctggct tgaagcaacg cgttgtgacg ttgccgcctt gggcgcgtcg
     1981 acagatcacg ccatggcgcc cttgtccgac gacgcgccac ggcgaatcaa gccctgcacc
     2041 cctttcgcgg acctgcatcc cctttgtcag taaacctgcc attattgtgg tccttccagc
     2101 gaggcccacc cgtcaggaca acaactattc ctgcagcgta acgctttggt gacagaaatg
     2161 cccaatttgt gctgtttttg gtaaacattt ctaagatttg atcgtccctg ttcaaactat
     2221 cgctccatca atacgccggc tttaagcatt ggcgcgccca gcccgaggct ctgcgcggag
     2281 ccctggcgca cgatcgcctc ggccggcgta gccgggcaca tcctcgcgcg ccgccggcac
     2341 cgagaaggcg tgggtccaga ggtcctcatc ctcggcattg acgacatcga ccggaagcaa
     2401 atagttga
//
LOCUS       DASTMT010000226         5264 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_450789, whole genome shotgun sequence.
ACCESSION   DASTMT010000226 DASTMT010000000
VERSION     DASTMT010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 5264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5264
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_450789"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1542
                     /gene="phaC"
                     /locus_tag="VFR00_05280"
     CDS             <1..1542
                     /gene="phaC"
                     /locus_tag="VFR00_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009491520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I poly(R)-hydroxyalkanoic acid synthase"
                     /protein_id="HEU0158698.1"
                     /translation="LQLGTASMQRLMGGEAPPVVEPEPGDNRFKDPDWSTNPYFDFWK
                     QSYLLTTRWLEDLLERTEGLDDRTRQRAEFYLKQLASALSPSNFPLTNPEVLRETFAS
                     SGHNLVQGMANLAHDMDKSGDILSISQTDTEAFEVGRNIATSPGKVIFQNDIVQLIQY
                     APSTDSVYQRPLLIVPPWINKFYILDLGPQKSFIRFAVSKGFTVFVVSWVNPDTRLAH
                     KTFEDYMTEGILSATDAVKRETGSDKVNVIGYCVGGTLLGTTLAYLAARGEEPFASAT
                     FFAAQVDFTKAGDLMLFIDDAQLKALEEMMAERGYLDGSRMATVFNLLRPKDLIWPYI
                     VNNYMLGKKPFPFDLLFWNQDSTRMPAANHGFYLREFYHENKLARGQMSIAGVKLDLG
                     KIKLPIYELCAKEDHIAPAKSVFIGSKLFGGPVTYVLAGSGHIAGVINPPDRPKYQFW
                     TNDKKAATLEAWLEGAQEHPGSWWPHYAEWLAKHSGGQVKARRPGESLGVIEDAPGSY
                     VKVKS"
     gene            complement(1526..1804)
                     /locus_tag="VFR00_05285"
     CDS             complement(1526..1804)
                     /locus_tag="VFR00_05285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0158699.1"
                     /translation="MTLSRMAAALGLLLTLATTAQACDSYADDMALASVLARAVAEAR
                     AQGALPRLATATPEPAVPAPGEPASGSPEPERTGAAARAFVAGLSSSP"
     gene            complement(1966..3039)
                     /locus_tag="VFR00_05290"
     CDS             complement(1966..3039)
                     /locus_tag="VFR00_05290"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEU0158700.1"
                     /translation="MGQASPRPALASSLKEDCVLLRLSVLDQSVALLDRPHSESIANT
                     LALAQHCESLGYQRFWLSEHHNHGSIVGTAPEILIAAIATHTRAIRVGSAGVMLPHYS
                     AFKVAEQFRVLDALAPGRVDLGLGRAPGSDGRTAFALNPLANERPAQFPGDVRDLLAW
                     VTGSALPEGHPFRQLRAYPEGQTSPEVWILGSSDYGAQVAAHFGLPYAFAWFFTDGKG
                     GPEALELYRQLYRPSARHPVPHTALCVWALAAASAEEANYHFASRARVRLLRDRGIFA
                     PLEAPEAALAHPTTADERARLAEFQREAFVGSADQVVERIAGLAQRLKVDEMVVVTWA
                     YDEQARRQSYQLLAKSAGLVSRP"
     gene            3244..3930
                     /locus_tag="VFR00_05295"
     CDS             3244..3930
                     /locus_tag="VFR00_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016840895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HEU0158701.1"
                     /translation="MLKFYYNGSPNPTKVALFLEEAGLAYEPIPIDTRKGDQFKGEFL
                     SINPNGKVPAIVDGDVTVFDSNAILLYLAEKTGKFLPERTERARGDLLSWLMFVATGV
                     GPFSGQAVHFRHFAPEPVPYAANRYSFEVERHYGILDQRLGKQRYMLGETYTIVDMNV
                     WGWTRLGPFVLGEEGAQRFANLKRLIDEISARPAAKRAVALKDRHSFKTEMDDEARRV
                     MFPHNARLAR"
     gene            4063..>5264
                     /locus_tag="VFR00_05300"
     CDS             4063..>5264
                     /locus_tag="VFR00_05300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3604 domain-containing protein"
                     /protein_id="HEU0158702.1"
                     /translation="MPYSTYRPELMGTAELTPSGAFEAGSFQSFSLVYTAGAFGIDDT
                     GSIKIGFRFATDFGPLQFSDPEAQGYTSVEASNGATLETKWEFKRNIRPWSRSLYIGV
                     VRDFLAPGDTITVRFGDPRFGSPGVRLQTYCESAFEFRVFADPIATYDYVALPNSPQI
                     ALVPGPGVRLAAVLPSLVRAGEPFRLALRAEDKWGNPSDREDRLVRLESEARILNLPA
                     SVRLRAGAFASIVEDLRAPAAGDLCIRVLDATGAVELCRSNPLRIAPADATLRHFWGD
                     THGQSNETLGTNTAREYFQFGRDKACLDVMGHQGNDFQISSSFWRELNALTREFDAPG
                     RFVCIPGYEWSANTALGGDRNVHYAQEGQSIHRSSQAQLVDRDEEAADALTAEALFAK
                     LKGKDCVV"
BASE COUNT          942 a         1813 c         1665 g          844 t
ORIGIN      
        1 ttgcaactcg gcaccgcttc tatgcagcgc ctgatgggcg gcgaggcgcc ccccgtggtg
       61 gagccggaac ccggggacaa ccgtttcaaa gaccccgact ggagcaccaa tccctacttc
      121 gatttctgga agcagtccta cctcctcacc acacgctggc tcgaagacct gttggagcgc
      181 accgaggggc tcgatgaccg cacgcgccag cgcgccgaat tctatctcaa gcaattggca
      241 agcgcgctgt cgccatccaa ctttccgctg accaaccccg aggtgctacg cgagaccttt
      301 gcctccagcg gccacaacct ggtgcagggt atggccaatc tcgcgcacga catggataag
      361 tcgggcgata tcctgagcat cagtcaaacc gacacggagg ccttcgaggt cggccgcaac
      421 atcgccacct cgcccggcaa ggtgatcttc cagaacgaca tcgtgcagct gatccagtac
      481 gcgccctcca ccgacagcgt ctaccagcgt ccgctgctga tcgttccccc ctggatcaac
      541 aagttctaca tcctcgacct cggtccgcag aagtccttca tccgcttcgc cgtcagcaaa
      601 ggcttcacgg tcttcgtcgt ctcctgggtc aatcccgaca cccggctggc gcacaagacc
      661 ttcgaagact acatgaccga gggcattctg agcgccaccg acgccgtcaa gcgcgaaacc
      721 ggcagcgaca aggtcaacgt gatcggctac tgcgtcggcg gcaccctgct cggcaccacc
      781 ttggcctatc ttgccgcccg cggcgaagag ccgttcgcat ccgccacctt ctttgccgcc
      841 caggtcgatt tcaccaaggc cggcgacctg atgctgttca tcgacgatgc ccagctgaag
      901 gcgctggagg agatgatggc cgaacgcggc tacctcgacg gctcgcgtat ggcgaccgtc
      961 ttcaacctgc tgcgcccgaa ggacctgatt tggccctaca tcgtcaacaa ctacatgctc
     1021 ggcaaaaaac cgttcccgtt tgatcttctg ttctggaacc aggactcaac ccgcatgccc
     1081 gccgccaatc acggctttta cctgcgggag ttctaccatg agaacaagct ggcccgcggc
     1141 caaatgtcga ttgccggcgt caaactcgac ctcggcaaga tcaagctgcc gatctacgag
     1201 ttgtgcgcca aagaagacca catcgccccg gccaagtcgg tgttcatcgg ctcgaagctg
     1261 ttcggcggcc cggtcaccta cgtgctcgcc ggctccggcc acatcgccgg cgtcatcaat
     1321 ccgccggacc ggccgaagta tcagttttgg accaacgaca agaaggcggc gaccttggag
     1381 gcctggctcg agggcgcgca ggagcacccg ggctcctggt ggccgcacta cgccgaatgg
     1441 ctggcgaagc attcgggcgg gcaggtaaag gcgcgccggc ccggggagag cttgggcgtg
     1501 atcgaggacg cgcccggtag ctacgtcaag gtgaagagct gagcccggcc acgaaggccc
     1561 gtgcggcggc acccgtccgc tccggctcgg ggctccccga cgcgggctcg ccaggggctg
     1621 ggacggcagg ctccggcgtt gcggtcgcca gccgcggcag cgcgccctgg gcgcgcgcct
     1681 cggcgacggc gcgcgccaga accgaagcca gcgccatgtc atcggcgtag ctatcgcacg
     1741 cctgcgccgt ggtagcgagt gtcagaagca atcccaaagc ggcggccatc ctgctgagcg
     1801 tcatgtgagg tctcctgtca cccgtctcct gtcgcccaag tgagcgccaa cagtgcgggc
     1861 tgcgttgagc ccaggcaagt gagaaaaagc ttccgacccg agcgcgtcgc cgcacgggcc
     1921 ggcgcgagcg cacgggcccg cttctggcct caggcgccgc cggcctcaag ggcggcttac
     1981 cagtccggcg cttttggcca acagctgata gctttgccgg cgcgcctgct cgtcataggc
     2041 ccaggtcacg accaccattt cgtcgacctt caggcgctgc gccaggccgg cgatacgctc
     2101 gacgacttga tcggcgctgc cgacgaaagc ttcgcgctga aactcggcga gccgtgcccg
     2161 ctcgtcagcc gtcgtgggat gggccagcgc cgcctcgggg gcctcgagcg gcgcaaagat
     2221 gccgcggtcg cgcaacagac gcacgcgcgc ccgggaggcg aaatgatagt tggcctcctc
     2281 cgcgctggcg gcggccagcg cccaaacgca cagcgccgtg tgcggcacgg gatggcgcgc
     2341 actgggccga tagagctggc ggtagagctc gagggcttcc gggccaccct tgccgtcggt
     2401 gaaaaaccag gcgaaggcat agggcaagcc gaaatgcgcc gccacctgcg ccccgtaatc
     2461 ggagctaccg agaatccaaa cctcgggcga ggtttgcccc tcggggtagg cgcgcagctg
     2521 ccggaaggga tgcccctccg gcaaggccga acccgtcacc caggccaaga ggtcacgcac
     2581 gtcgccggga aattgcgccg gacgctcgtt ggcaagcgga ttgagcgcga aggcggtgcg
     2641 cccgtccgag cccggcgcgc gcccaaggcc gagatcgacg cgcccgggtg cgagggcgtc
     2701 gagcacacga aactgctcgg cgaccttgaa ggcggagtag tgcggcagca tcacgccggc
     2761 gctgccgacc cggatcgccc gggtgtgcgt ggcgatggcg gcaatcaaga tctccggcgc
     2821 ggtgccgaca atgctgccgt ggttgtggtg ctcggaaagc cagaagcgct ggtagcccag
     2881 gctttcgcag tgctgcgcca gcgccagggt gttggcgatg gattcgctat gcggacgatc
     2941 gaggagggca accgattgat cgagcaccga cagacgtagc aggacgcaat cctccttcag
     3001 ggaagaagcc agggcgggcc tcggcgacgc ttggcccata acgccggctc ccgggctcta
     3061 aggccccctt ccccggagaa tatggggcgg cggcggcgcg cgccagccgc cgcatcgcct
     3121 catgacgcgc aggcgctgcg ccgcgcgccc tccctataac ctgagccggc ctttgcccac
     3181 cccggcgcgg tgacataacc tccaccgcac ccggccacgg gaccctgcaa ctggagacgg
     3241 acgatgttga aattctacta caacggttca cccaacccca ccaaggtcgc cctcttcctc
     3301 gaggaagccg ggcttgccta cgagccaatc ccgatcgaca cgcgcaaagg cgaccagttc
     3361 aaaggcgagt tcctcagcat caatcccaac ggcaaggttc ccgccattgt cgacggtgac
     3421 gtcaccgtct tcgacagcaa tgcaattctt ctctatctcg ccgaaaagac cggcaagttt
     3481 ttacccgagc gcacggaaag ggcgcgcggc gacctgctct cttggctgat gttcgtcgcc
     3541 accggcgttg ggcccttctc ggggcaggcc gtgcacttcc gccatttcgc gcccgagccc
     3601 gtgccctacg ccgccaatcg ctacagcttc gaagtcgagc gccactacgg cattctcgat
     3661 cagcgcctcg gcaagcaacg ttacatgctg ggggagactt acacgatcgt cgatatgaac
     3721 gtgtggggct ggacgcggct tggccccttc gtcttgggcg aggagggcgc gcaacgcttt
     3781 gccaacctga agcggctgat cgacgagatc tccgctcgac ccgcggccaa gcgcgcggtg
     3841 gccttgaagg accggcacag cttcaagacc gagatggacg atgaggcgcg ccgcgtcatg
     3901 ttcccgcaca acgcgcgcct ggcacgttag caccgccgcc gctgcggctc gaggccgagc
     3961 ggcggcctgg cggcccctcg cctcgagcca agccagcgcg gccgccgcgc gggcgcgcgc
     4021 agaaaacgtc gcattgcccc tgcccgccag cgaggagcgc cgatgcccta ttcgacttac
     4081 cgacccgagc tcatgggaac ggccgagctc actccgagcg gcgccttcga ggcaggctcc
     4141 tttcagagct ttagcttggt ttacacggcc ggcgccttcg gtatcgacga taccggctcc
     4201 atcaagatcg gcttccgctt tgccacggat ttcgggccat tgcagttcag cgaccccgag
     4261 gcgcaaggct ataccagcgt ggaagcctcc aatggggcga cgctcgagac caagtgggag
     4321 ttcaaacgca acatccgccc ctggagccga tcgctttata ttggcgtggt gcgggacttc
     4381 ctggcgcccg gcgacaccat caccgtgcgc ttcggtgatc cgcgtttcgg cagcccgggc
     4441 gtgcgcctgc agacctactg cgagagcgcc ttcgagtttc gcgtcttcgc cgatccgatc
     4501 gccacctacg actacgtcgc gctgccgaat agtccgcaga tcgcgctcgt gccggggccg
     4561 ggggtgcggc tcgcagccgt gctgccgagc ctggttcgcg ccggcgaacc gttccgcttg
     4621 gcgctcaggg ccgaggacaa gtggggcaat ccgagcgatc gcgaggaccg cctggtccgc
     4681 cttgaaagcg aggcgcgcat cctcaacctg cccgccagcg tgcgcctgcg ggccggagcc
     4741 ttcgccagca tcgtggagga cttgcgcgcc ccggcagccg gcgacctttg catccgcgtc
     4801 ctcgatgcga cgggggcggt ggagctgtgc cgctccaatc ccttgagaat tgcgccggcc
     4861 gatgcgacct tgcgccattt ctggggcgat acgcacggtc agtcgaacga gaccttgggc
     4921 accaatacgg cgcgcgagta ctttcaattc ggtcgcgaca aggcctgcct ggacgtgatg
     4981 gggcatcaag gcaacgactt ccagatctcc agcagcttct ggcgtgaact caacgcgctc
     5041 acccgcgaat ttgatgcgcc cggccggttc gtgtgcattc ccggctacga atggtcggcc
     5101 aacacggcgc tggggggtga tcgaaacgtg cactacgctc aagaggggca gagcatccac
     5161 cgctcctccc aggcccagct cgtcgaccgc gacgaggagg ccgccgacgc gctgaccgcc
     5221 gaggccctgt tcgccaagct caagggcaag gactgcgtgg tgat
//
LOCUS       DASTMT010000227        11865 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_778734, whole genome shotgun sequence.
ACCESSION   DASTMT010000227 DASTMT010000000
VERSION     DASTMT010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 11865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11865)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11865
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_778734"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1251)
                     /gene="mmsA"
                     /locus_tag="VFR00_05305"
     CDS             complement(<1..1251)
                     /gene="mmsA"
                     /locus_tag="VFR00_05305"
                     /EC_number="1.2.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019861803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004491 - methylmalonate-semialdehyde
                     dehydrogenase (acylating, NAD) activity [Evidence IEA];
                     GO_process: GO:0006574 - valine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEU0158703.1"
                     /translation="MIDYGHFIGGKPVKGTSGRSGEVFQPMTGEIRAKVALAAEAEVA
                     LAVEHAKAAQPGWAATNPQRRARVLMRFLELAQRDYDRLADCLAREHGKTVADAKGDL
                     QRGLEVVEFSLGVPHLMKGEYTEGAGPGIDLYSMRQPLGVVAGITPFNFPAMIPLWQC
                     APAIACGNAFILKPSERDPGVPLALAELLLEAGLPPGIFNVINGDKVAVDAILDHPDI
                     AGVGFVGSTQVAQYIYARGSAQGKRMQCFGGAKNHMIVMPDADMDQAVDALIGAGYGS
                     AGERCMAVSVAVPVGKATAETLVEKLIPRVESLKIGPSTSADAEMGPLVTAAHLKKVK
                     DYVGLGLEEGAHLKVDGRDFRMQGYENGFYMGGCLFDAVEPNMRIYKEEIFGPVLSVV
                     RAATYEESLKLANDHEYGNGVAIFT"
     gene            1559..3568
                     /locus_tag="VFR00_05310"
     CDS             1559..3568
                     /locus_tag="VFR00_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006932254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEU0158704.1"
                     /translation="MTGTSASQSVAAAHDDALDTPPKLLLFNARVHGQRPAIRHKDFG
                     IWQTWSWARLLAEVRALSIGLRKLDLERGDTVAIIGDNRPRLYATFAAVQSLGAIPVP
                     VYQDSVAEEMAYVLEHAEVKIAVVEDQEQVDKIISVANRVPHLKKIIYDDPRGLKDYD
                     AGNLHAFSDVQALGEEEMRSNPGAEAWWRDEIGKGQGSDISVVLYTSGTTGRPKGVML
                     THANIVISARNGNIFDNFGPDDTLLAYLPMAWVGDHLFSYGQAYAGAMCVCCPESPET
                     AVEDRREIGPTYFFAPPRVFENLLTQIMVRMEDAGAIKRRLFHYFLGVARRCGEDILN
                     GKKVGPVDRLLYRLGDLLFFAPLRNRMGFSRTRVAYTAGEAIGPELFRFYRSLGINLK
                     QLYGQTEASVYITLQPDGEVYPDSVGKPGPDVEIKIAENGEVLFRSPGVFLKYHKNEE
                     ATRATKTPEGWVHTGDAGFFDQRGHLKIIDRAKDVGRLSGGALFAPKYLENRLKFYPE
                     IREAVAFGQGEDYATMLVNIDQISVGNWAERNGVAYASYQELAAHPDVYAIVKARVDE
                     LNRALAQEPQMAASQIRRFLILHKELDADDGELTRTLKVRRSFISERYAPLIKALYDG
                     SSSCHVATEMTFEDGRKGVVEGEVKIVDMAVHAARPPQPMREAAE"
     gene            3565..4389
                     /locus_tag="VFR00_05315"
     CDS             3565..4389
                     /locus_tag="VFR00_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009764468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0158705.1"
                     /translation="MKADVQPRPVLGKETSVAAGEVLLSLEHVSLSFGGVKALRDVSF
                     DIRKGEIRAIIGPNGAGKTSMLNVINGFYHPQEGVITFKGRRRHNMRPHLAASQGIAR
                     TFQNVALFKGMSTLDNIMTGRTLKMRRGILAHMLHWGPAQREEIRHREAVEHIIDFLE
                     IAHIRKIPVGKLPYGLQKRVELGRALAMEPELLLLDEPMAGMNLEEKQDMSRFILTVK
                     DSFDTTIALIEHDMGVVMDLSDRVVVLDYGRKIADGSPAAVQNDQAVIDAYLGVAH"
     gene            4403..5332
                     /locus_tag="VFR00_05320"
     CDS             4403..5332
                     /locus_tag="VFR00_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEU0158706.1"
                     /translation="MLLAGQVLEVLLGGLLSGVLYSLVALGFVLIFKASGVFNFAQGA
                     MVLLAGLAMVRCLDYLSHHEFPLWAAVLLALAFAAGIMALTAWLIERFVLGPLVNQDG
                     LTLFMATIGATFVLEGASQMIFGSDVYPLRLFPTDAWIIGESMFPGGILINKLDVWGG
                     LIAGVLVAGLAIFFQNTKTGRALRAVADDHAAAQSVGIPISWIWFVVWLVAGLVALVA
                     ATVWGTKLGVQFSITFLALKALPVLIIGGFTSVPGAIVGGLMVGAGEKLAEIYLGPHI
                     GGGIEYWFAYVLALLVLLIRPQGLFGERIIERI"
     gene            5343..6470
                     /locus_tag="VFR00_05325"
     CDS             5343..6470
                     /locus_tag="VFR00_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016840290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEU0158707.1"
                     /translation="MLYREAGQFKESYAADMAIFPIRQDRIALLLLLAFAYVGVPLLA
                     EFHIWPFGSDYLLRAILIPFLILALGSIGLNILMGYCGQISLGTGAFMAIGAYSAYKF
                     ATGVHLPLAWIGEDISIPALPVLASILLGGLMAALVGILFGIPSLRIKGLYLAVATLA
                     AQFFFDWVFIRVKWFTNYTPSGSVTAPELKILGLTFSTPTERYLLCLLFVSVFALMTK
                     NMVRGNLGRQWMAIRDMDIAAELMGIRPLYAKLSAFAVSSFVVGVAGALWAFVYLGAW
                     EPLAFNIDRSFQLLFMVIIGGLGSILGSFLGAAFILILPIALDQAPHALGIPITVETV
                     SLLVLIITGALICYLLIVEPLGFARLWSIAKEKLRLWPYPY"
     gene            6612..7928
                     /locus_tag="VFR00_05330"
     CDS             6612..7928
                     /locus_tag="VFR00_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011993137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0158708.1"
                     /translation="MHSKTLGLAAALVAAAALAAPAAAQNEQFLPALVYRTGPYAPNG
                     IPFADGVADYWTLLNERDGGINGVKIVFEECETGYATDKGVECYERLKGKGPTGAGYF
                     SPLSTGITFALTEKVPGDKIPLLTTGYGRSESREGSVFTWNFIALGSYWTAADIAIQH
                     IAKELGGADKLKGKKITLVYHDSPYGKEPIPALEARAKLNGIEFKAVPVTHPGVEQKS
                     QWLAIRQDRPDYVLLWGWGVMNSTAIKEAAAVAYPRDKMIGVWWSGAEPDVTPAGDQA
                     VGYKALMLQHPAGKFKVHEDLEKFVISKGKGAGKDDFAQVLYNRGIINSMLGTEAIRT
                     AQGKFGKKPLTGEQIRWGFENLNLTADRLKELGFEGMLKPIKFSCQDHQGADQARLQQ
                     WDGKQWKIISDFYTADESVLAPMVKETAAKYAKEKNITPRDCAKES"
     gene            8007..8861
                     /locus_tag="VFR00_05335"
     CDS             8007..8861
                     /locus_tag="VFR00_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007008835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0158709.1"
                     /translation="MSDAPSQSPSIPPAAATQSVLLGVKNIEVIYDHVILVLKGVSLT
                     VRSGSIVALLGANGAGKSTTLKAISNLIRGERGEVTKGSIEFAGERIDRLTPNDLVRR
                     GCIQVMEGRHCFGHLSVEENLLTGAFTRRDGRGAIAADLELVYGYFPRLKERRASLAG
                     YISGGEQQMVAIGRALMSRPKMMLLDEPSMGLAPQLVEEIFEIVKRLNAEQHVSLLLA
                     EQNTNMALKYATYGYILESGRVVMDGQAHDLRENEDVKEFYLGIGGGDRKSFRNVKSY
                     KRRKRWLA"
     gene            8945..10180
                     /locus_tag="VFR00_05340"
     CDS             8945..10180
                     /locus_tag="VFR00_05340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006021582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEU0158710.1"
                     /translation="MTDHFDQLEIRDPEQRELAQFNLLPDLIRHALAHAPGWADHLKG
                     IDPAALSSREALAAVPVLRKSALKDLQAKRPPYGGFATSAAGTLARVFMSPGPIFEPE
                     GHGEDWWRAARALYAAGIRKGDIVHNTFAYHLTPGGWILDSGARALGCTVIAAGPGNS
                     EQQLEAIERLKPTAYVGVPDYLKILLDKAKETRKDATSLKKALVGGGALFPSLRAEYK
                     QRGIATYQTYATADLGIIAYESPALEGMIVDEGAILEIVRPGTGDPLPAGEVGEVVVT
                     TFNRDYPMIRFATGDLSAVLPGVSPCGRTNMRIKGWLGRADQTTKVKGMFVHPEHVAE
                     IAKRHAGLGRLRLVVGRAGEQDTMLLKAESASPDSALKGKLADSLQAITKLKGDVELV
                     APNSLPNDGKVIADERTYN"
     gene            complement(10244..11458)
                     /locus_tag="VFR00_05345"
     CDS             complement(10244..11458)
                     /locus_tag="VFR00_05345"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018641485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEU0158711.1"
                     /translation="MKFGIFYELQLPRPWLPNGELKLYQDALTQVELADRLGYDYAWM
                     VEHHFLEEYSHCPAPESFLGAASQRTRQIRLGHGILQLTTSHPARVAERVAVLDLLSN
                     GRCEFGMGESASITELEPFGRAMEDKRAVFEEAVAAILPMFKEGGSEHHGKYFDIPLR
                     NVVPKPIQKPHPPLWMACSQLDTIERAGRCGLGALGFQFVSADAAHAWVHAYYNAMTK
                     RLAKLADYSINPNIALVSFFMCGKTDEEARKKADGATFFQFALRFYGASSDRKRPAPG
                     TVNMWDEYTAWKRANPAAQEAALRGGLIGSPDTLRKKLAKFETSHIDQVILLNQAGKN
                     AHEDIAASLELFAKEVMPEFQGHHAQQQAWKEKVMAGEIELEEIETAAFRDRYGGKSM
                     PVQAIEKAAVRR"
     gene            complement(11509..>11865)
                     /locus_tag="VFR00_05350"
     CDS             complement(11509..>11865)
                     /locus_tag="VFR00_05350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011580394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU0158712.1"
                     /translation="LNTGAKAPPASGAPTAVTRAAGMALTKVLAGEGAPHNVLVNAVL
                     VGRIESDQWVRRHAAEGKGRTLGDYYDEMGKSIPMGRIGTAEEFANMVLFLASDAGSY
                     ITGTAINIDGNLSPVV"
BASE COUNT         2122 a         3845 c         3744 g         2154 t
ORIGIN      
        1 ggtaaagatc gccacgccgt tgccatactc gtggtcattg gcgagcttga ggctctcctc
       61 gtaggttgca gcccgcacga cggacaggac cggcccgaaa atctcttctt tgtagatgcg
      121 catgttgggc tcaacagcat cgaacaggca gccgcccata tagaagccgt tctcgtagcc
      181 ttgcatgcga aagtcgcgac catcgacctt caggtgagcg ccctcctcga ggccgaggcc
      241 gacgtagtcc ttgaccttct tgagatgcgc ggcggtcacc agcggcccca tctccgcatc
      301 ggccgacgtc gagggcccga tcttcagcga ctccacgcgc ggaatgagct tttcgacgag
      361 cgtctcggca gtggccttgc cgaccggcac cgccacggac accgccatgc agcgttcgcc
      421 ggcggagccg tagcccgcgc cgatcagggc atcgacggct tgatccatgt cggcatcggg
      481 catgacgatc atgtggttct tggcgccgcc gaaacactgc atgcgctttc cctgcgccga
      541 gccacgcgca taaatgtatt gggcgacctg cgtcgagccg acgaagccga cgccggcaat
      601 gtcgggatgg tcgagaatgg catcgaccgc caccttgtcg ccgttgataa cgttaaagat
      661 cccgggcggc agtcccgcct ccagcagcaa ctcggctagc gccaagggta ccccggggtc
      721 acgctccgag ggcttgagga taaaggcatt gccgcaggca atcgccgggg cgcactgcca
      781 gagcgggatc atggccggga agttgaacgg tgtgatgccg gccacgacgc cgagcggctg
      841 gcgcatggag taaaggtcga tacccggccc cgcaccttcg gtgtactcgc ccttcatcag
      901 gtgcggcacc cctaaggaaa attccacgac ctcgaggccg cgttgtagat cgcccttggc
      961 gtcggcgacc gtcttgccgt gctcgcgggc gaggcaatcg gcgagcctgt catagtcgcg
     1021 ctgcgccaac tccaaaaagc gcatcagcac gcgggcacgg cgctgcggat tggtggcggc
     1081 ccagcccggt tgggcggcct tggcgtgttc gacggcaagc gccacctcgg cctcggccgc
     1141 caaggcgacc ttggccctaa tctcccccgt catcggctgg aacacctcgc cgctgcggcc
     1201 gctcgtccct ttcaccggct tgccgccgat gaaatgcccg taatcgatca tgggtcacac
     1261 tcccggttca cgccgccatt aacccatgcg ctaacatggg cccgccgcag ctcgcaaggt
     1321 cggcccgggg ggcccaatgg cacgcgcagc ggcaaggggc gccgtggccc gggcaagcgg
     1381 cggggcgggc attgcggggc cgcaaagccg ggctctcgga ctaaagttca agcttcgggc
     1441 ggcgtgccag cccctgccgc ccaattgcac cgggcgggcc ccgacgataa aagaccaaac
     1501 aaaaaaagcg cccactcagg atggcgcggt gcggcccgtt cagggagggt gatgaagcgt
     1561 gactgggacc agtgcctctc aaagcgtggc cgctgcccac gacgacgccc tcgatacgcc
     1621 acccaagctc ctcctcttca atgcccgcgt gcacgggcag cgacccgcca ttcgccacaa
     1681 ggacttcggc atctggcaga cctggagttg ggcacgtttg ctcgcggagg tgcgcgcgct
     1741 ttcgatcggc ctgcgcaaac tcgatcttga gcgcggcgac acggtcgcca tcatcggcga
     1801 caatcgccca cgcctttatg ccacattcgc cgccgtacag agcctcggcg caatcccggt
     1861 gcccgtctac caggactcgg tggccgagga gatggcctac gtcctcgagc acgccgaggt
     1921 gaagatcgcc gtcgtggaag atcaagagca ggtcgacaag atcatctcgg tggccaatcg
     1981 cgtgccgcat ctcaagaaga tcatctacga cgacccgcgc ggtctcaagg actacgacgc
     2041 cggcaatctg catgccttca gcgacgtgca ggcgctgggc gaagaggaga tgcgcagcaa
     2101 ccccggcgcg gaggcttggt ggcgcgacga gatcggcaag ggccaaggca gcgacatcag
     2161 tgtcgtgctc tatacctccg gcaccacggg cagacccaag ggcgtgatgc tgacgcacgc
     2221 caacatcgtc atctcggccc gcaacggcaa catcttcgat aacttcggtc ccgacgacac
     2281 cctgctcgcc tatctgccga tggcttgggt tggcgatcat ctattctcct acgggcaggc
     2341 ctacgccggg gccatgtgcg tgtgctgccc ggaaagtccg gaaacggcgg tagaggatcg
     2401 gcgcgaaatc ggccccacct atttttttgc cccgccgcgc gtattcgaaa acctgcttac
     2461 gcaaatcatg gtgcgtatgg aggatgccgg cgccatcaag cgccgcctgt tccactattt
     2521 ccttggcgtt gcccggcgct gcggtgagga catcttgaac ggcaaaaagg ttggccccgt
     2581 cgaccgcctc ctctatcgcc tcggcgatct gctctttttc gcgccgctca ggaaccgcat
     2641 gggcttctcg cgcacgcgcg tcgcctacac ggccggcgag gccatcggcc cggagctgtt
     2701 tcgtttttat cgctcgctcg gcatcaatct caagcagctc tacggtcaga ccgaggccag
     2761 cgtctacatc accctgcagc ccgacggcga ggtctatccc gacagcgtcg gcaagcctgg
     2821 acccgacgtg gaaatcaaga tcgccgagaa cggcgaggtc ctgttcagga gcccgggtgt
     2881 gttcctcaag taccataaga acgaggaggc cacgcgcgcg accaaaacgc cggagggctg
     2941 ggtgcacacc ggtgacgccg gtttcttcga ccagcgcggg cacttgaaga ttatcgatcg
     3001 ggccaaggac gtcggccggc tcagcggcgg ggcgctgttc gcgccaaagt acctggagaa
     3061 ccggctcaaa ttttaccccg agatccggga agcggtcgcc ttcggccagg gcgaagacta
     3121 tgccacgatg ctggtcaata tcgatcaaat ctcggtcggc aactgggccg agcgcaatgg
     3181 cgttgcctat gccagctacc aggagctggc cgcccacccc gacgtctatg ccatcgtgaa
     3241 ggcccgcgtc gacgagttga atcgggcgct ggcgcaggaa ccgcaaatgg ccgcttccca
     3301 gatccgccgc tttctgatcc tccataagga gctggacgcc gacgacggcg agctcacccg
     3361 cacgttgaag gtgcgccgct catttatctc ggaacgctac gcccccctga tcaaggccct
     3421 ctacgacggc tcgtcctcct gtcacgtcgc cacggagatg accttcgagg acggacgcaa
     3481 gggcgtggtc gaaggcgaag tgaagatcgt cgacatggcg gtgcatgcgg cgcgcccacc
     3541 gcaaccgatg cgggaggctg ccgaatgaaa gccgacgtgc aacctcgccc cgtgctgggg
     3601 aaagagacca gcgtggcggc gggcgaggtc ctgctttcac tcgagcacgt ttcgctttcc
     3661 tttggcggcg tcaaggcgct gcgtgatgtc tctttcgaca tccgcaaggg cgaaattcgc
     3721 gccatcatcg gtcccaacgg cgccggcaag acctcgatgc tcaacgtcat caacggcttt
     3781 taccatccgc aggagggcgt catcaccttc aagggcaggc gccgccacaa catgcgcccg
     3841 catctggccg ccagccaggg cattgcccgc accttccaaa acgtcgcctt gttcaagggt
     3901 atgtcgacgc tcgacaacat catgactggg cgtaccctga aaatgcgccg cggtattctc
     3961 gctcacatgc tgcactgggg accggcccag cgcgaggaga tccgccatcg cgaggcggtt
     4021 gagcacatca tcgacttcct ggagatcgcg cacatccgca aaatccccgt cggcaagctt
     4081 ccctacggcc tgcaaaagcg cgtggagctc gggcgcgcgc tcgccatgga accggaactg
     4141 ctgttgctgg acgagccgat ggcgggcatg aacctggaag agaagcagga catgagccgc
     4201 ttcatcctga ccgtcaaaga cagcttcgac accaccatcg ccttgatcga gcacgacatg
     4261 ggggtggtga tggacctgtc cgaccgcgtg gtggtgctgg actatggccg caagattgcg
     4321 gacggctcgc cggcggcggt gcagaacgac caggccgtga tcgacgccta tctcggcgtg
     4381 gcgcactagg gagttgggtg gcgtgctact ggcgggccaa gttttggaag tgctgctcgg
     4441 cggcctgttg tccggcgtgc tttattccct ggtggccttg ggtttcgtcc tgatcttcaa
     4501 ggcctcgggc gtgttcaact tcgcccaggg cgccatggtg ctgttggcgg ggctggccat
     4561 ggtgcgctgc ctcgactacc taagccacca tgaatttccg ctctgggcgg cggtgttgtt
     4621 ggcgctggcc ttcgccgccg gcatcatggc gctgacggct tggctcatcg agcgtttcgt
     4681 gctggggccg ctcgtcaacc aggacggcct cacgctcttt atggccacca tcggcgccac
     4741 cttcgtgctg gagggcgcct cgcagatgat cttcggctcc gacgtttatc ccttgcggct
     4801 ttttcccacc gacgcctgga tcatcggcga gagcatgttc cccggtggga tcttgatcaa
     4861 caagctcgat gtctggggcg gtctcatcgc cggcgtcctg gttgcgggcc tggcgatttt
     4921 ctttcagaac accaagacgg gccgcgcgct acgcgcggtg gccgacgatc acgccgcggc
     4981 gcaatcggtc ggcatcccca tcagctggat ctggttcgtg gtctggctgg tggccggtct
     5041 ggtggcgctc gttgccgcca ccgtctgggg caccaagctc ggggtgcaat tctccatcac
     5101 gtttctggcg ctcaaggccc tgccggtcct catcatcggc ggcttcacct cggtgccggg
     5161 ggccatcgtc ggcggcctga tggtgggagc gggcgagaag ctcgccgaga tctaccttgg
     5221 cccacacatc ggcggcggca tcgagtattg gttcgcctac gtcttggcct tgctggtgct
     5281 gctgatccgg ccgcaaggac tgtttggcga acgcatcatc gagcgcattt agggagaaag
     5341 ccgtgcttta tcgcgaggcg ggccaattca aggagagcta cgccgccgac atggcgatct
     5401 tcccgatccg ccaggatcgc atcgccctcc tattgctgct ggccttcgcc tacgtcggcg
     5461 tgccgttgct tgccgagttc cacatttggc cgtttggcag cgactatctg ctgcgggcga
     5521 tcctgatccc ctttttgatt ttggcgctgg ggagtatcgg cctcaatatt ctgatgggct
     5581 actgcggcca gatctcgctc ggcaccggtg ccttcatggc gatcggcgcc tatagcgcct
     5641 ataagtttgc caccggcgta catctgcctc tcgcctggat cggcgaagac atatccattc
     5701 ccgccttgcc cgtgctggcc tcgatcctgc tcggtggcct gatggcggcg ctggtcggaa
     5761 ttctgttcgg catcccgagc ctgcgcatca aggggcttta cctggcggtg gccacgctgg
     5821 ccgcacagtt tttcttcgat tgggttttca tccgcgtgaa gtggttcacc aactacacgc
     5881 cgtccggctc ggtcacggca cccgagttga agatcctcgg cctcaccttt tccacgccga
     5941 ccgagcgcta tctgttgtgc ctgctgttcg tcagcgtgtt cgcgctgatg accaagaaca
     6001 tggtgcgtgg caaccttggc aggcaatgga tggcgatccg ggatatggat atcgccgcgg
     6061 agctgatggg catcaggccg ctctacgcca agctgtcagc cttcgccgtc tcctccttcg
     6121 tagtgggcgt cgccggcgcg ctgtgggcct tcgtctatct cggcgcctgg gagccgctgg
     6181 ccttcaacat cgatcgctcc ttccagctcc tgttcatggt gattatcggc gggttggggt
     6241 cgatcctggg ctcgttcctg ggggcggcgt tcatcctgat cctgccgatt gcgctcgacc
     6301 aggcgcccca tgccctcggc atccccatca ccgtcgaaac cgtatcgctg ctggtgctga
     6361 tcatcaccgg cgccttgatc tgctaccttc tgatcgtaga acccttgggc tttgccaggc
     6421 tgtggtcgat cgccaaggaa aaactgaggc tctggcccta cccctactaa ggggtgggcg
     6481 ctccaatgcc ggcgcgcagt gcggagcggc gcgcgtggta accaaagacg tgaggcccgc
     6541 gcacgacggc gctcgttctc atgcgccaac acaaagggtc cggcacccgc cgggatgaag
     6601 gaggaatgtc gatgcacagc aaaactcttg gccttgccgc ggcgctcgtc gcagcagcgg
     6661 ctttggccgc accggcggcg gcgcagaacg agcagtttct gccggcgctg gtctatcgga
     6721 ccggccccta cgcccccaac ggcattccct tcgccgacgg cgtcgccgac tactggacgc
     6781 tcttgaacga gcgtgacggc ggcatcaacg gcgtcaaaat cgtgttcgag gagtgcgaga
     6841 cgggctacgc caccgacaag ggtgtggagt gctacgaacg cttgaagggc aagggcccga
     6901 ccggtgcggg atatttctcc cccctgtcga cgggcatcac cttcgcgctg acggaaaaag
     6961 tgccgggcga caagatcccc ctgctgacca ccggctacgg ccgctcggaa agccgcgagg
     7021 gctcggtgtt cacctggaat ttcattgcgc tcggcagcta ctggacggcg gccgatatcg
     7081 ccatccagca cattgccaaa gaactgggcg gcgcggacaa gctcaagggc aagaagatta
     7141 ccctcgtcta ccacgacagc ccctacggca aggagccgat cccggccttg gaggcacggg
     7201 ccaagctcaa cggcatcgag ttcaaggcgg tccccgtgac gcaccccggc gtggagcaga
     7261 aatcgcagtg gctcgccatt cgccaggatc gtccggacta tgtcttgttg tggggctggg
     7321 gcgtcatgaa ttccaccgcc atcaaggagg cagccgcggt cgcctatcct cgcgacaaga
     7381 tgattggcgt gtggtggtcg ggggccgagc ccgacgtaac gccagccgga gaccaagccg
     7441 tcggctacaa ggccctgatg ctgcagcatc ccgcgggcaa attcaaagtg cacgaggatc
     7501 tggagaagtt cgtcatttcc aagggcaagg gtgccggcaa ggacgacttc gcccaggtgc
     7561 tttacaatcg cggcattatc aactccatgc tgggaacgga ggccattcgc acggctcagg
     7621 gcaagttcgg caagaagcca ttgacgggtg agcagatccg ctggggcttc gagaacctca
     7681 atctcaccgc ggaccgcctc aaggagctcg gtttcgaagg catgctgaag ccgatcaaat
     7741 tctcctgtca ggatcatcag ggcgccgacc aggcacggct ccagcagtgg gacggcaaac
     7801 agtggaagat catctccgac ttctacacgg ccgatgaaag cgtgctggcg ccgatggtga
     7861 aggagaccgc ggccaaatac gccaaggaaa agaacatcac gccgcgcgac tgcgccaaag
     7921 aaagctaagg aaccgacggg ggaggccgtg tggcctctcc cacgcctgcc ttagactggg
     7981 ctcgagcccc gcggagttga cgtttcgtgt ctgatgctcc ttcccaatcg ccctcgatcc
     8041 cgccggcggc cgcgacgcag agcgtcctgc tcggggtcaa gaacatcgag gtcatctatg
     8101 accacgtgat cctggtgctg aagggtgtgt cgctgacggt gcggtccggc agcatcgtcg
     8161 ccctgcttgg cgccaatggt gccggcaaat ccacgacctt gaaagcgatc tccaacctga
     8221 tccgcggcga acgcggtgag gtgaccaagg gctccatcga attcgccggc gagcgcatcg
     8281 atcggctgac cccgaacgat ctggtgcgcc gcggttgcat tcaggtcatg gagggacgcc
     8341 actgcttcgg gcatctttcc gtagaggaaa acctcttgac cggtgcgttc acgcgccggg
     8401 acggccgcgg cgccatcgcc gccgacctgg aactggtgta cggctacttt ccgcgcctga
     8461 aagagcgccg cgcctcgctc gcgggctaca tctctggcgg cgaacagcag atggtggcga
     8521 ttggccgcgc cctgatgtca cgcccaaaga tgatgctgtt ggacgaaccg tcgatggggc
     8581 tggcgccgca gctggtggaa gagatcttcg aaatcgtcaa gcgcctcaat gccgagcagc
     8641 acgtctccct gctgctggcc gagcaaaaca ccaacatggc gctgaaatac gcaacctacg
     8701 gctacattct ggagagcggc cgcgtcgtta tggacggaca agcccatgac ctgcgcgaaa
     8761 acgaggacgt gaaggagttc tacctcggca ttggcggcgg cgatcgcaag agcttccgaa
     8821 acgtcaagtc ctacaagcgc cgcaagcgct ggctggcttg atgcgcctgg gagcctctgg
     8881 cagtgttgtg gtgagccgct gcccacgata gggaggccca cctgacgata gggatgccca
     8941 cattgtgacc gaccatttcg accagctcga aatccgcgac cctgagcagc gcgaactggc
     9001 ccagttcaac ctgcttcctg acctcattcg ccatgcgctt gcgcatgcgc cgggctgggc
     9061 ggaccacctc aagggcatcg atcctgcggc cctgagctcg cgcgaggcgc tcgccgccgt
     9121 gcccgtgctg cgcaagtctg cgctcaagga cctgcaggcg aagcggcccc cctatggcgg
     9181 ctttgccacc tcggcggccg gcaccttggc gcgcgtattc atgtcgcccg ggccgatctt
     9241 cgagccggag gggcatggcg aggattggtg gcgcgcagca cgcgcgctct atgcggccgg
     9301 gatccgcaag ggcgacatcg tgcacaatac ctttgcctat cacctgacgc cgggcggttg
     9361 gatcctcgat tcgggcgcgc gcgcgctcgg ctgcacggtg atcgcggccg ggcccggcaa
     9421 ctccgagcag cagctcgagg ccatcgagcg tttgaagccg accgcctacg ttggcgtgcc
     9481 cgactatctc aagatcctgc tcgataaggc gaaggaaacg cgcaaggacg ccacatccct
     9541 caagaaggcg ctggtcggcg gcggtgcgct gttcccctcg cttagggccg agtacaaaca
     9601 gcgcggcatt gccacctacc agacgtatgc caccgccgac ctcggcatca tcgcctatga
     9661 aagccccgcg ctcgagggca tgatcgtcga tgagggcgcg attctcgaga tcgtccggcc
     9721 cggcaccggc gatcccttgc cggcgggcga ggtaggcgag gtggtggtga ccacgttcaa
     9781 tcgcgactac ccgatgatcc gctttgccac gggcgacctt tcggcggtgc tgcccggcgt
     9841 cagcccctgc gggcgcacca acatgcgcat caagggctgg ctcggccgcg cggatcagac
     9901 caccaaggtc aagggcatgt tcgtgcaccc cgaacacgtt gccgagatcg ccaagcgcca
     9961 cgccggacta ggccggctgc ggttggtggt ggggcgcgcc ggcgagcagg acaccatgct
    10021 cctgaaagcg gaaagcgcca gccccgacag cgcgctcaag ggcaagctgg cggacagtct
    10081 gcaagccatc accaagctga aaggcgacgt cgagctggtg gcaccaaact cgttgcccaa
    10141 tgacggcaag gtgattgccg acgagcgcac ctacaattga gctgagaccg gccccgcgag
    10201 cttgaacgtc cgaggcctgg accggggcag cggtgcgggc gtctcagcgc cgcacggcgg
    10261 ccttctcgat cgcctgcacc ggcatcgatt taccgccgta gcggtcgcga aaggctgcgg
    10321 tctcgatctc ttcgagctca atctcgcccg ccatcacctt ctccttccag gcctgctgct
    10381 gggcgtggtg gccttggaac tcgggcatca cctccttggc aaacagttca agggaggcgg
    10441 cgatgtcctc gtgtgcgttc ttgccggcct ggttcagcag gatcacctga tcgatgtggg
    10501 aggtttcgaa tttcgccaat ttcttgcgca aggtgtcggg cgagccgatc agaccgccgc
    10561 gcagtgccgc ctcctgcgcc gccggattgg cgcgcttcca ggcggtatac tcatcccaca
    10621 tgttgaccgt gccgggcgcg ggtcgcttgc ggtcgctaga ggcgccgtaa aagcgcagcg
    10681 cgaactgaaa gaaagtggcc ccgtccgcct ttttgcgtgc ctcctcgtcg gtcttgccgc
    10741 acatgaaaaa gctgaccagc gcgatgttgg gattgatgct gtagtcggcg agctttgcca
    10801 agcgcttggt catggcattg tagtaggcat gcacccaggc atgcgccgca tcggcactga
    10861 cgaactgaaa gcccagcgcc cccagcccgc agcgcccggc acgctcgatg gtgtcgagct
    10921 gcgagcaggc catccacaat ggcgggtgcg gcttctggat cggcttgggc accacgttgc
    10981 gcaagggaat atcgaaatac ttaccgtggt gctcgctacc accctcctta aacatgggta
    11041 ggatggcggc gaccgcctcc tcgaacacgg cgcgcttgtc ctccatggcg cggccgaagg
    11101 gttcgagctc ggtgatcgac gcgctctcgc ccatgccgaa ctcgcagcgg ccgttgctca
    11161 aaagatcgag gacggcgacg cgctcggcca cacgcgcggg gtgggacgtc gtgagctgca
    11221 ggatgccgtg gccgagccgg atttgccgcg tgcgctggct ggcagcgccc agaaagctct
    11281 ccggggcggg acagtgcgag tattcctcga ggaaatggtg ttcgaccatc caggcatagt
    11341 cgtagccgag ccggtcggcc agctcgacct gcgtcagggc atcctgatag agcttgagct
    11401 cgccattggg cagccatggc ctcggcaact gcagctcgta aaagatgccg aacttcatgc
    11461 cctgcctccc tcgcggctca aacggcaaga tagcggagcc atcgcgccct acacaaccgg
    11521 gctcagattg ccgtcgatgt tgatcgcggt gcccgtgata taggagccgg catcggaggc
    11581 aagaaacagc accatattgg cgaattcctc ggcggtgccg atgcgaccca tcgggatcga
    11641 cttgcccatc tcgtcataat aatccccgag cgtgcggccc ttgccctcgg cggcgtggcg
    11701 ccgcacccac tgatcgctct cgatacgccc caccagcacg gcattgacga gcacgttatg
    11761 gggcgccccc tcgccggcca agaccttggt caatgccatc ccagccgcgc gcgtcaccgc
    11821 cgtcggcgcg ccggatgcgg gcggcgcctt ggcccccgta ttgag
//
LOCUS       DASTMT010000228        12691 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_2744746, whole genome shotgun sequence.
ACCESSION   DASTMT010000228 DASTMT010000000
VERSION     DASTMT010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 12691)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12691)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12691
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2744746"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..321)
                     /locus_tag="VFR00_05355"
     CDS             complement(<1..321)
                     /locus_tag="VFR00_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018699505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU0158713.1"
                     /translation="MSDPNSTPPLPAPKTRRGERTRQKILNAAQREIGSKGFAEASIS
                     TITAQAAVGQGTFYLYFRSKEDVLRELVLRMGRRLRRHLTLAIAAAPNRLEAERRGLR
                     AFLEF"
     gene            478..1680
                     /locus_tag="VFR00_05360"
     CDS             478..1680
                     /locus_tag="VFR00_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009764234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEU0158714.1"
                     /translation="MPKVHAAALGAVAVLGAMGLATAPALAKDVKICLIAGKTGALEA
                     YAKQTENGFLMGLEYLTQGSNKVGNDKLEVIVKDDQLKPDRGKSLLEECYGDDKADLA
                     VGTTGSPVALAMLPVAEEHKKVLIVEPAVADSITGDKWNRYIFRTGRSSYQDALAGAA
                     AFPKSGEVAVGMLGLDTAFGRDGIAAFKQAIASIRPNVKVVAEEYAAGNTADFAPFAE
                     RLFNALKDKPGKKVIGFIWAGPHPMAKFVDMKPERFGIELAPGGNILPVMNAWKQFAG
                     TEGGIYYYYDFPKNAENDWLKAEYQKRYGAPPDFFVAGGFAAAAAVVAALKQAGSSDS
                     EKLIAAMEGLTFNTPKGPMTFRKEDHQALQDMYHFRIKKDAKDNDVLELVGTIAANEM
                     PLPVRTKR"
     gene            1811..2584
                     /locus_tag="VFR00_05365"
     CDS             1811..2584
                     /locus_tag="VFR00_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012047416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0158715.1"
                     /translation="MSIPVLETRELSIRFGGHLAVAGVSAAFRAGTLTAIVGPNGAGK
                     TTYFNLISGQLPASAGSVLLAGRNITPLKAAARARLGLGRAFQLTNLFPQLSVHENVR
                     LAVQAKDGGGGNIASRWTRRTDWIDRADRFLAAADLSDKRDLRAATLPHGDKRKLEVA
                     IMMALEPHVFMFDEPTAGMSVGDVPVILDLIRQLKAEGSKTILLVEHKMDVVRSLADR
                     IIVLHNGRLVADGAPAEVIGSAIVQEAYLGIAAGSASHG"
     gene            2577..3302
                     /locus_tag="VFR00_05370"
     CDS             2577..3302
                     /locus_tag="VFR00_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011662826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0158716.1"
                     /translation="MAEPLLTLAGVHTHIGQYHILQGVDFVVPEGELTVLLGRNGAGK
                     TTTLRTIMGLWQPSRGQVSFAGQAIGGLPAYAIARAGIAYVPESMAVFSDLSVEENML
                     LAARDAPLQRARLDWVFSFFPALKRFWTHPAGTLSGGQKQMLSIARAVIEPRRLLLID
                     EPTKGIAPAIIDNMIRAFRELKRERTTILLVEQNFRFAEALGDWAVVMDDGRIVHRAG
                     MADLARDGATQRRLLGLSLEAQA"
     gene            3409..4473
                     /locus_tag="VFR00_05375"
     CDS             3409..4473
                     /locus_tag="VFR00_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011662825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEU0158717.1"
                     /translation="MLSSEDRAASAAAPDRSADGLEPIPKDEADYLPLLLLPALAAGA
                     LAIMLWQGVPLSTWITLTIAALAMGAMIFVIASGLTLVFGLMDVLNFGHGAFIAVGAY
                     LAMTTLLPLAGWVEANSLLLNLAALGLAMGAAMLGTGVLGLAFERIIIMPVYGQHLKQ
                     ILITTGGLIVAEQLIHVVWGGDQKPMPLPSALRGAIVVGNVALEKFRLLAMAIGLLVF
                     LGMLLLLNRSKLGLLIRAGVENSEMVEALGYRIRRLFAVVFVAGCALAGLGGTLWALY
                     RETLTASIGSEVIVLVFIVIIIGGLGSVGGCFLGALLVAQMANYTGYLAPKLALASNI
                     GLMVAILMWRPQGLYPVAKR"
     gene            4481..5569
                     /locus_tag="VFR00_05380"
     CDS             4481..5569
                     /locus_tag="VFR00_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009764230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEU0158718.1"
                     /translation="MLRALLSDDLPGSRLLSALLLAILLLLALAPLLFSGAQAINTAA
                     KICVFIVLVASYDLLLGYTGIVSFAHTMFYGIGGYGVGLALYGWGATWAAAGAGLAVA
                     LLSGIALALLIGLFSLRVRAIFFAMITLAVASAFAILAAQLSEYTGGEDGRSFRVPEL
                     LRPSYVLTELPLLGPVTGRTLAYYLVFVSAFVLFLAALRIVNSPFGRVLQAIRENDFR
                     AEALGYRTVSYRTAANCLAAAMAVLAGGLNALWLRYTGPDTTLSFSIMVDILLMVVIG
                     GMGTMYGAVIGASLFVLAQNYLQKLMATAAQATEAVPLLAKLLHPDRWLLWLGILFVL
                     SVYFFPTGIVGKLREAKTSPRLRAPAHP"
     gene            5681..6172
                     /locus_tag="VFR00_05385"
     CDS             5681..6172
                     /locus_tag="VFR00_05385"
                     /inference="COORDINATES: protein motif:HMM:NF024106.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative molybdenum carrier protein"
                     /protein_id="HEU0158719.1"
                     /translation="MKIISGGQSGVDRAALDVAIERGLDWGGWCPKGGWAEDFPEPPG
                     LLTKYPHLKETEHSHPLQRTEWNVRDSNATLILTDKEGLAPSIGTARALQWARQHGKP
                     FLVLDATDPTAPARAAAWFKQLTHRFGPELTLGVGGPRESEAPGIYQLAKNLLSASLD
                     LRH"
     gene            6406..8055
                     /locus_tag="VFR00_05390"
     CDS             6406..8055
                     /locus_tag="VFR00_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEU0158720.1"
                     /translation="MRHGGKILIDQLEAQGASSAFTVPGESFLAALDGLHDSNRIKTI
                     ICRQEGGASMMAEAWGKITGEPGVCFVTRGPGLANAMSGLHVAQQDSTPMVLFVGLPA
                     SGVEDREAFQEIDSKSLLGSFVKWAAVIRQTERIPEYVSHAYHMARSGRPGPVVLGLP
                     EDMLAAECEASDAAPARIADSRPAAEDLAELERLLAKATRPVLILGGPGWSTAAKAAL
                     EAFAERFQLPVACAFRYQDYFDNRHPCYVGCAGIGIEPTLGKAIKEADLLLVIGARLG
                     EMTTGGYALLKIPNPSQRLVHVHPSADELGSVYRADLPIVAATHRFAEALGRLQPAFE
                     IGWGAHTQTLRAAYQSFLEPISMPGSVKLAEVVRTASELLPQDAFISNGAGNYAAFLH
                     RYFEYKTYRTQLAPTSGSMGYGLPAAIAAKIAHPDRVVVNFQGDGCFLMTGQELATAV
                     QYGLGIITIVANNGMYGTIRMHQEREYPRRVVGTTLVNPDFAAYARSFGADGYSIETA
                     ADFAPAFKSALNSNRPAVIELKLDPEAISPRLTLAALRQRA"
     gene            8226..8396
                     /locus_tag="VFR00_05395"
     CDS             8226..8396
                     /locus_tag="VFR00_05395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0158721.1"
                     /translation="MDLRTAEQLNGKLWQHAKAGALLCFLIASLLLLASLLSPMPEEI
                     SNAVAATTSALP"
     gene            complement(8441..9367)
                     /locus_tag="VFR00_05400"
     CDS             complement(8441..9367)
                     /locus_tag="VFR00_05400"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018431258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEU0158722.1"
                     /translation="MQQQRHFSGVIAPVLTPFGEDGGPDPERFVAHCQWLMQEGCTGL
                     APFGTTSEGNSLGVDERMELLEELADSDVDAATLMPGTGACALADAIVLTRHAVDVGC
                     GGVLMLPPFYYKAPNEEGLFRFFAEVIEGVGDDRLKVYLYHIPPVAQVGFSLSLIGRL
                     IKAFPDTVVGLKDSSGDWSNTAAILEAYPQFEVFPGSEIFLLDGLRKGAAGCISATCN
                     VSAAAIRNVYDSWRTAEADALQTGITALRKAIQAYPMIPALKAIIAHYRQDPVWARLR
                     PPFTELPAADAAKAIETLAQAHGFRLDFAKAA"
     gene            complement(9406..10038)
                     /locus_tag="VFR00_05405"
     CDS             complement(9406..10038)
                     /locus_tag="VFR00_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008196659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HEU0158723.1"
                     /translation="MIDLYTWSTPNGRKVSIMLEECGLEYAAHAVNITKDEQFKPEFL
                     KISPNNRIPAIIDRDNGRSLFESGAILLYLAEKTGKFWPQEPDRRWQTVEWLMWQMGG
                     IGPMIGQVHHFVRNNKGKAPYAEERYVKETHRLYGVLDKRLADRDYVAGVYSIADMAI
                     WPWISRFEWHTVDLNQYPNVARWYKAIAARPAVIKGYDVPVKQPGIPMPA"
     gene            complement(10230..11237)
                     /gene="glpX"
                     /locus_tag="VFR00_05410"
     CDS             complement(10230..11237)
                     /gene="glpX"
                     /locus_tag="VFR00_05410"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HEU0158724.1"
                     /translation="MKNTIQGHDVVGGLDRVLVLEVARVTEAAAIAAARLRGRGDEKA
                     ADKAAVDAMRHELGKVHIKGRVVIGEGEMDESPMLYIGEEVGLGDGPEVDIAVDPLEG
                     TTICAKAMPNALAVLAISERGGLLNAPDMYMNKIAIGPGYPDGIIDLDAPPAETLRAM
                     AKARGVAISEITACILDRPRHAELIASVRKAGAGVQLIPDGDIAGVIWTTDPKETGID
                     IYFGIGGAPEGVLAAAALRCIGGQMQGRLHPLKKEEETRAAAMGVSDIRRKYDMHELA
                     SGDVIFSATGVTDGSLLDGVHFRGDFAETETVVLRSKTSTVRRIKTTFRMIGTKTSPG
                     I"
     gene            complement(11277..12602)
                     /locus_tag="VFR00_05415"
     CDS             complement(11277..12602)
                     /locus_tag="VFR00_05415"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HEU0158725.1"
                     /translation="MSQPLKLGIAGLGTVGAGLVQLIQEHGARLALTSGREITIAGVS
                     ARSRQKKRSVTLEGVAWFEEAERLATDPGIDVFIELIGGEEGVARAAVEAALKAGKHV
                     VTANKALLAKHGTELASLAEARAVALKFEAAVAGGIPVIKTLREALAGNQIRRVYGIL
                     NGTCNYILTQMQEEHRAFAEVLKEAQDKGYAEADPTFDIGGFDTAHKLALLTSLAFGT
                     KVALDQIHVEGIQGISQADIEAADDLGYRIKLLGVALLTDSGIEQRVHAAMVPKHSAI
                     AEVSGVTNCIAIDGDFVGNVLLAGPGAGAKATASAVAADVLDIARGGFAHPFGVPAAE
                     LKPYRRARLGQHQGAYYVRLSVFDRPGAMASIAGRMGERGVSLESIVQRRPRWALPGI
                     DARPKPGAPTTVILITHETTEEAIRAALNAIHADGNVSEPPQMIRIEPL"
BASE COUNT         2087 a         4251 c         4103 g         2250 t
ORIGIN      
        1 gaactccaaa aacgcccgca agccgcggcg ctcggcctcg agccggttgg gggccgccgc
       61 gatcgccaag gtcaggtgac ggcgcaaccg gcgccccatg cgcaatacca gctcgcgcag
      121 gacgtcctcc ttgctgcgga aatatagata gaaggtgccc tggccgaccg cggcttgcgc
      181 cgtgatggtg ctgatggagg cttcggcgaa gcccttgctg ccgatttcgc gctgggcggc
      241 attgaggatc ttttgccgcg tgcgctcgcc gcgccgggtc ttgggcgccg gcaggggcgg
      301 cgtagaattt ggatcagaca tgagcacttt gtcatgttgc atgcagcaca atgccggcgg
      361 agcctggaga ctctttggcc ttgacggcca atttgggcga cgttagcgta aactgaaacc
      421 tgactcaata ctcaggttta ttggagagga cgcgagaccg tcgctgagga ggtcgttatg
      481 ccaaaggttc atgccgcggc gctgggggcg gtggccgtgc tcggcgcgat ggggctcgcc
      541 acagccccgg cgctcgccaa ggacgtcaaa atctgcctga tcgccggcaa gaccggcgcg
      601 ctggaagcct acgccaagca gaccgagaac ggctttctga tgggtcttga gtatctgacc
      661 cagggcagca acaaggtcgg caacgacaag ctcgaggtca tcgtcaagga cgatcagctc
      721 aaacccgacc gcggcaagtc gctgctggaa gagtgctacg gcgacgacaa ggccgatctc
      781 gccgtcggca ccaccggctc gcccgtcgcg ctggccatgc tgccggtggc cgaggagcac
      841 aaaaaggtgc tgatcgtcga accggcggtg gccgacagca tcaccgggga caagtggaac
      901 cgctatatct tccgtaccgg ccgcagctcc tatcaggacg cgctggccgg cgccgccgcc
      961 ttcccgaaaa gcggtgaggt cgccgtcggc atgctcggcc tcgacacggc gtttggacgc
     1021 gacggcatcg ccgctttcaa acaagccatt gccagcatcc ggccgaacgt gaaggtggtg
     1081 gcggaggaat atgcggccgg gaatacggca gactttgcgc ctttcgccga acggctcttc
     1141 aacgccttga aggacaagcc cggcaagaaa gtgatcggct tcatttgggc cggcccgcat
     1201 cccatggcga agttcgtcga catgaagccc gagcgcttcg gcatcgaact ggcgccgggg
     1261 ggcaacattc tccctgtcat gaatgcgtgg aagcagttcg ccgggaccga aggcggcatc
     1321 tactattatt acgacttccc caagaacgcc gagaacgatt ggctcaaggc cgagtaccag
     1381 aagcgctacg gcgccccgcc cgatttcttc gtcgccggcg gatttgcggc ggccgcggcc
     1441 gtggttgcgg cgctgaagca ggccggctcc agcgacagcg agaagttgat tgccgccatg
     1501 gagggcctca ccttcaatac acccaagggg ccgatgacct tccgcaagga agaccatcag
     1561 gcgctgcagg acatgtatca cttccgcatc aagaaggacg ccaaggacaa cgacgtcttg
     1621 gagctcgtgg gcaccatcgc ggccaacgaa atgccgttgc cggtgcgcac gaagcgctga
     1681 ggccgagcgc cacgcggcgc gcggggaccc cggacaatcc gcccccggcg ccgcaacacc
     1741 cccggcaagc gcggtttccg tcacggacac cgccttgcgc ccactccccg gcaagggcgg
     1801 ctcgtcgctg atgagcatac ctgtgttgga gacacgcgag ctcagcattc gcttcggcgg
     1861 ccacctcgcc gtcgccgggg tgagcgcggc ctttcgggcc ggcacactca cggccatcgt
     1921 cgggcccaac ggcgccggca agaccaccta tttcaacctg atctcggggc aattgccggc
     1981 gagtgccggc agcgtgctcc tcgccggtcg caacattacc ccgctcaaag ccgccgcgcg
     2041 cgcccgcctg ggtcttggcc gggccttcca gctgaccaac ctctttccgc aactcagcgt
     2101 gcacgaaaat gtccgcctcg ccgtgcaggc caaggacggg ggcggcggca acatcgcctc
     2161 gcgctggacg cgccgcacgg actggatcga tcgcgccgat cgctttcttg ctgcggccga
     2221 tctgtccgac aagcgcgatc tgcgcgctgc aaccttgccg cacggcgaca agcgcaagct
     2281 cgaagtggcg atcatgatgg cgctcgagcc gcacgtcttc atgttcgatg aaccgaccgc
     2341 gggcatgtcg gtcggcgacg tgccggtaat cctcgacctg atcaggcagc tcaaggccga
     2401 ggggagcaag acgattttac tggtcgagca caaaatggac gtggtgcgca gcctcgccga
     2461 tcgcatcatc gtgctgcaca acggtcgcct ggttgcggat ggagcacccg ccgaggtgat
     2521 cggctcggcg atcgtgcagg aggcctatct cgggattgcc gcggggagtg ccagccatgg
     2581 ctgagccgct gttgaccctt gccggcgtgc atacgcacat tgggcagtac cacatcctgc
     2641 agggcgtcga cttcgtcgta ccggaaggcg agttgaccgt gctgctcggg cgtaacgggg
     2701 caggcaagac caccacgctg cgcaccatca tgggcttatg gcagccgagc cggggtcagg
     2761 tgagctttgc cggccaggcc atcggcggtt tgcccgccta cgccatcgcc cgtgccggca
     2821 tcgcctacgt gccggaaagc atggccgtgt tctccgactt gagcgtggag gagaacatgc
     2881 tgctggcggc acgcgatgcc ccgctgcaac gcgcccggct cgactgggtg ttttccttct
     2941 tcccggcgct gaaacgtttc tggacgcatc cggccggtac gctttcgggc gggcaaaagc
     3001 agatgctgtc catcgcccgc gcggtgattg agccgcgacg cctgctgctg atcgatgagc
     3061 ccaccaaagg catcgcgccg gcgatcatcg acaacatgat ccgggctttt cgcgagttga
     3121 agcgcgaacg cacgaccata ctgctggtgg agcagaattt tcgcttcgcc gaggccctgg
     3181 gcgattgggc ggtcgtcatg gacgacggcc gcatcgtgca tcgcgccggc atggccgatc
     3241 ttgcccgcga cggggccacc cagcgtcgcc tgctggggtt gtcgctggag gcccaggcat
     3301 gatcgaggct gccacaccgc tcccctgcaa ccccatgacc cggcgcgcag caccggcgcc
     3361 acggcgccgt gggcaagcga acagcgcacc tgagccacga ggcgcgcaat gctgagctcc
     3421 gaggatcgcg ccgcgagcgc cgccgccccc gaccgctcgg ccgacgggct cgagccgata
     3481 cccaaagacg aggccgatta tctgcccctc ctgctgttgc ccgcgctcgc cgccggcgcg
     3541 ctcgccatca tgctgtggca gggcgtaccg ctgagcacct ggatcacgct gaccatcgct
     3601 gccctcgcca tgggtgccat gatcttcgtg atcgccagcg gcctgacctt ggtgttcggc
     3661 ctgatggatg tcctcaattt tggccatggc gcctttattg cggtgggcgc ctatctcgcc
     3721 atgacgacgc tgttgccgct ggccggctgg gtcgaggcca actcgctgct cctcaatctc
     3781 gccgcgctcg gcctcgccat gggagcggcg atgctcggca ccggcgtgct cgggctcgcc
     3841 ttcgagcgca ttattatcat gcccgtttac ggccaacacc tgaagcagat cctgatcacc
     3901 accggtgggc tgatcgtggc cgagcagctg atccacgtgg tctggggcgg cgaccagaaa
     3961 ccgatgccgc tgccgagcgc gctgcgcggc gccatcgtcg tcggcaatgt cgccttggag
     4021 aagtttcgtc tgctcgccat ggccatcggc ctcctcgtct tccttggcat gctgctgctg
     4081 ctcaatcgca gcaagctcgg cttgttgatt cgcgccggcg tcgagaatag cgagatggtc
     4141 gaagcgctcg gctaccgcat ccgccggcta ttcgccgtag tgttcgtggc aggctgcgcg
     4201 cttgctggct tgggcggaac cctgtgggcg ctctaccgcg agaccctgac ggccagcatt
     4261 ggcagcgagg tgatcgtgct ggtgttcatc gtcatcatca tcggtggcct gggctcggtc
     4321 ggcggctgct ttctcggcgc cctattggtc gcgcaaatgg ccaactacac cggctatctc
     4381 gcgcccaagt tggcgctcgc ctccaacatc ggactgatgg tggcgatcct gatgtggcgc
     4441 ccgcagggcc tctatcccgt agccaaacgt tgagccggcc atgctgcgtg cgctcctctc
     4501 cgatgatttg ccaggcagcc ggctgctatc ggcgctgttg ctggcgatcc tcctcttgct
     4561 ggcgctcgcg cccttgctgt tcagcggcgc ccaggccatc aacacggcgg ccaagatttg
     4621 cgtcttcatc gtgttggtgg cgagctacga cctcttgctc ggctatacgg gcatcgtctc
     4681 cttcgcccac accatgttct acggcatcgg cggctatggc gttggcctgg cgctttacgg
     4741 ctggggggcg acgtgggctg ccgccggcgc cgggctcgcc gtcgccttgc tcagcggtat
     4801 cgccttggcc ttgctgatcg gtctcttctc cttgcgggtg cgggcgatct tttttgccat
     4861 gatcacgctg gcggtggctt ccgccttcgc cattctggcc gcccagcttt ccgaatacac
     4921 cgggggcgag gatggccgtt cgtttcgggt gcccgagctg ctgcgcccgt cctacgtctt
     4981 gaccgagttg ccgctcctcg gcccggtcac gggccgcacg ctggcctact atctggtctt
     5041 cgtcagcgcc tttgtgctct tcctggcggc gctgcgcatc gtcaattcgc ccttcggccg
     5101 cgtcctgcag gccattcgtg agaacgactt ccgcgccgag gcgctcggct accgcacggt
     5161 cagctatcgc accgccgcca actgtctcgc cgcggcaatg gccgtgctgg ccggcggtct
     5221 caacgccctg tggctgcgct acacggggcc cgataccact ctctccttca gcattatggt
     5281 cgacatcctc ttgatggtgg tgatcggtgg catgggcacc atgtacggcg ccgtgatcgg
     5341 cgcgagcctc ttcgtcttgg cccagaacta cttgcaaaag ctgatggcga cggcagccca
     5401 ggccaccgaa gcggttccac tgctggccaa gctgttgcat ccggaccgat ggctgctctg
     5461 gctcggcatc ctgttcgtgc tgagcgtcta tttttttccc accggcatcg ttggcaagct
     5521 gcgcgaggcg aaaacctcgc cgcggctcag agccccggcc cacccctgaa aaccaggcgg
     5581 cgcggtcgag ctggcgcccg ccggcggcga ttggcgggcg ggccgcgagc caggtagcga
     5641 cgggattgtg tttcccgcgc atttggccat gatgggtggc atgaagatca tcagtggcgg
     5701 ccagagcggc gtcgaccgtg ccgcgctgga tgtggccatc gagcgcggtc tcgactgggg
     5761 cgggtggtgc ccgaaaggcg gctgggccga agatttccca gagccgccgg gcctgcttac
     5821 gaaatatccc cacctcaagg agaccgaaca ctcccacccc ctgcagcgca cggaatggaa
     5881 cgtgcgcgac agcaacgcca ccttgatctt gacggacaag gaaggtctcg ctccctcgat
     5941 cggcacggcg cgggcgctgc agtgggcgcg ccagcacggc aagccttttc tcgtcctcga
     6001 cgcaaccgat cccaccgccc cggcgcgggc cgcggcctgg ttcaagcagc tcacgcatcg
     6061 atttggcccc gagctgacgc tgggcgtcgg tggtccccgc gaaagtgagg cccccggcat
     6121 ctaccagctc gccaaaaacc tcctctccgc cagcctcgat ttgcgccatt gaagggagca
     6181 ttgccgccgg caacgcgggc atcctgcgtt gcgcccacgc gggcctccct tgccaggcgc
     6241 ggcggcaagc tcatggtggg ccgccagcgc gtcctcgccc gttgacagcg cgcaaaccct
     6301 tcaagaatgt cggccttgca cacacgcgcg cgcctcgagc ccgtggcgca ggcgaacggg
     6361 gacccctggg gccctatcga tatcgctttg tggggagagg aaggcatgcg gcacggcggc
     6421 aagatcctga tcgatcaact cgaggcgcag ggagccagca gcgctttcac cgtgcccggg
     6481 gagagctttc tcgccgcctt ggatggcctg cacgacagca atcgcatcaa gaccatcatc
     6541 tgccggcagg agggcggcgc ctccatgatg gccgaagcct ggggcaagat cacgggcgag
     6601 cccggcgtct gttttgtcac gcgcgggccg gggctcgcca acgccatgag cgggctgcac
     6661 gtggcgcagc aagactccac gcccatggtg ctgtttgtcg gcctgcccgc gtccggcgtg
     6721 gaggaccgcg aggccttcca agaaatcgac agcaaaagcc tgctcggctc gttcgtgaag
     6781 tgggccgccg tcattcgcca gaccgagcgc atccccgaat acgtgagcca tgcctaccat
     6841 atggcgcgtt caggccggcc ggggccggtc gtgctgggcc tgcccgagga catgctggcg
     6901 gccgaatgcg aagccagcga tgcggcgccc gcccgcatcg ccgacagccg cccggcggct
     6961 gaggatttgg ccgagctcga gcgcctattg gcgaaggcaa cgcggcccgt gctgatcctc
     7021 ggcggacccg gttggagcac ggccgccaag gcggcgctcg aggcgttcgc ggagcgtttt
     7081 cagctgccgg tggcctgcgc cttccgctac caggattact tcgacaaccg ccatccctgc
     7141 tacgtcggct gcgccggcat cggcatcgag ccgaccctcg gcaaggccat caaggaggcg
     7201 gacttgttgc ttgtgatcgg ggcccgcctc ggtgaaatga caaccggtgg ttacgccttg
     7261 ctcaagatcc ccaaccccag ccaacgcctg gtgcacgtgc acccatccgc cgatgagctg
     7321 ggctcggtct atcgcgccga tctgccgatc gtggccgcca cgcatcgctt cgcggaggcg
     7381 ctcggccgtc tccagcccgc cttcgagatt ggctggggcg cacacaccca gaccttgcgc
     7441 gccgcctatc agagctttct cgagccgatc agcatgccgg gctcggtcaa gctggccgag
     7501 gttgtacgca cagcctccga gctcctgccc caggacgcct tcatcagcaa cggcgccggc
     7561 aactatgccg ccttcctgca ccgctacttc gaatacaaga cctaccgcac ccagctggca
     7621 cccacctcgg ggtcgatggg ctacggcctg ccggcggcga tcgcggccaa gatcgcgcac
     7681 ccggatcgcg tggtcgtcaa ttttcagggc gacggctgtt tcctgatgac cggccaggag
     7741 ctggccacgg cggtgcagta tggtttgggc atcatcacca tcgtcgccaa caacggcatg
     7801 tacggcacca tccgcatgca tcaagagcgc gagtacccgc ggcgcgtcgt gggcacgacc
     7861 ctggtcaatc cggatttcgc cgcctatgcc cgcagtttcg gggcggacgg ctacagcatc
     7921 gagaccgccg ccgactttgc cccggcgttc aagagcgcgc tcaacagcaa ccgaccggcg
     7981 gtgatcgaac tcaagctcga ccccgaggcg atcagcccgc gcctgacgct cgcggcgctg
     8041 cgccagcgcg cctgaagggc cgacgccgcc gcatgcaccg aaagggccat gcaacggttg
     8101 ccaaaggctt ggcaaattcg ttcgcctttg cctcaagctt ttcggattaa gaacgccagg
     8161 cggcgccgat agcctaatgg tcgattaggg attatggttt taaaagatcg gatgcggggg
     8221 attcgatgga tttgcggacc gcggagcaac tcaacggcaa gctttggcag cacgccaagg
     8281 ccggggcgtt gctctgcttt ttgatcgcga gcctgctctt gctcgcctcc ttgctttccc
     8341 ccatgcccga ggaaataagc aacgccgtgg cggccacgac gagcgcactg ccctaaagcc
     8401 tgcgcccgaa gcctgccccc cgacacctct gcgctcgagc tcaggccgcc ttggcaaaat
     8461 ccagcctaaa gccatgcgct tgggccagcg tctcgatcgc cttggcggca tcggccgcag
     8521 gcagctcggt aaagggcgga cgcaaccggg cccaaaccgg gtcctggcgg tagtgcgcga
     8581 tgatcgcctt cagcgccggg atcatcggat aggcctggat cgccttgcgc agcgcggtaa
     8641 tgccggtttg cagggcgtcc gcctcggcgg tgcgccagct gtcgtagacg ttgcggatcg
     8701 ccgcggcgct gacgttgcag gtggccgaga tgcagccggc cgcgcccttg cgcaggccat
     8761 ccaacaggaa gatttccgaa cccggaaaga cctcgaattg cggataggcc tcgagaatgg
     8821 cggccgtgtt gctccaatcg cccgaggagt ccttgaggcc gaccaccgta tcaggaaaag
     8881 ccttgatcag ccggccgatc agcgacaacg agaagccgac ctgggcgacg ggtggaatgt
     8941 gatagaggta gaccttgagg cggtcatcgc ccaccccctc gatcacctcg gcaaagaagc
     9001 ggaacagccc ctcctcattg ggcgccttgt agtagaacgg cggcagcatc agcaccccgc
     9061 cgcagccgac atcgacggcg tgccgcgtca gcacgatggc gtccgccagc gcacacgcgc
     9121 ccgtgcccgg catcaacgtc gccgcgtcga cgtcgctgtc ggcaagctcc tccaggagct
     9181 ccatgcgttc gtcgacgccc agcgagttgc cttcgctggt ggtgccgaag ggcgccagcc
     9241 ccgtgcagcc ctcctgcatc agccactgac agtgcgcaac gaagcgctcc gggtccgggc
     9301 cgccatcctc gccgaagggc gtcaacaccg gggcgatgac gccggagaaa tgcctttgct
     9361 gctgcatggg aaaacccttt ttaactgctt taactgcgct tggggtcagg ccggcatggg
     9421 aatgccgggc tgcttgacgg gaacgtcgta gcccttgatc acggccggcc gcgccgcaat
     9481 ggccttatac cagcgggcaa cgttggggta ctgattgagg tcgaccgtgt gccattcgaa
     9541 acgggagatc cacggccaaa tggccatgtc ggcgatgcta tagacgccgg ccacgtagtc
     9601 gcggtcggcc aagcgcttgt cgagcacgcc gtagaggcgg tgggtttcct tgacatagcg
     9661 ctcctcggcg tagggcgcct tgcctttatt gttgcgcacg aagtggtgca cttgcccgat
     9721 catcgggccg ataccgccca tttgccacat caaccactcc acggtctgcc agcgccgatc
     9781 gggctcctgc ggccagaact tgccggtctt ttccgccaga tagagcagga tcgcccccga
     9841 ctcgaagagc gaacggccgt tgtcgcggtc gatgatcgcg ggaatgcgat tattggggct
     9901 gatcttgagg aactcgggct tgaactgctc gtccttggtg atgttgacgg cgtgggcggc
     9961 gtattcgagg ccgcattcct ccaacatgat ggaaaccttg cgcccgttcg gggtcgacca
    10021 cgtgtaaaga tcgatcattg ccactcctcg ctcaccacag cgtctcaact gagcctcgac
    10081 ccttcttaga ccaaacggca agccgatgcc caccggaaag gtgtcgccgg ccctcgcgcg
    10141 cccgcacggc tgcacccgcg tgggggcggc gcggccgcgg cggatgcggc cgcctcgcgg
    10201 gggcgcaaag ccggctcggc ccgccttgcc tagatgcccg gggaggtctt ggtcccgatc
    10261 atgcgaaagg tggtcttgat gcggcgcacg gtgctggtct tggagcgcag cacgacggtt
    10321 tcggtttcgg caaaatcgcc gcgaaaatgc accccgtcga gcagcgagcc gtcggtcacc
    10381 ccggtggcgg agaagatcac gtcgccggag gccaattcgt gcatgtcata tttgcggcgg
    10441 atgtcgctga cccccatggc cgcggcgcgg gtttcctcct ccttcttgag gggatgcaac
    10501 cggccctgca tctgtccgcc gatgcagcgc agcgccgccg cggcaagcac gccctcgggg
    10561 gcgccgccga tgccgaagta aatgtcgata cccgtctcct tgggatcggt ggtccagatc
    10621 accccggcga tgtcgccgtc gggaatgagc tgcacacccg cgccggcctt gcgcacgctg
    10681 gcgatcagct cggcgtggcg gggacggtcg agaatgcagg ccgtgatttc cgagatcgcc
    10741 acgccacgtg ccttggccat ggcgcgcagc gtctcggcgg gcggcgcatc gagatcgatg
    10801 atgccatcgg gatagcccgg gccgatggcg attttgttca tgtacatgtc cggcgcattg
    10861 aggaggccgc cgcgctccga gatcgccagc acggcaagcg cattgggcat agccttggcg
    10921 cagatggtcg tgccctccag cggatcgacg gcaatgtcca cctccggccc gtcgcccagg
    10981 cccacctcct cgccgatata gagcatgggg ctctcgtcca tctcgccttc gccgatcacc
    11041 acccggccct tgatgtgcac cttgcccagc tcgtgccgca tggcatcgac ggcggccttg
    11101 tcggcggcct tctcgtcacc ccggcccctg agccgcgccg ccgcgatggc ggccgcttcg
    11161 gtgacgcgcg ccacctccag caccagcacg cgatctagtc cgccgacgac gtcatggccc
    11221 tggattgtgt tcttcatcgt gttctcccaa tcctagcaac cgcgccacct cggcccttac
    11281 agcggctcga tgcggatcat ctgtggcggc tcggaaacgt tgccatctgc gtgaatggcg
    11341 ttgagcgcgg cgcggatcgc ctcttccgtc gtctcgtgcg tgatcaatat cacggtggtg
    11401 ggtgcgccgg gcttgggacg ggcgtcgatg ccgggcagcg cccagcgcgg ccggcgttgc
    11461 acgatgctct ccaaggagac gccgcgctcg cccattcgcc cggcgatgga ggccatggcg
    11521 ccgggacggt cgaacaccga aaggcgcacg taataggcac cctggtgctg gccgagcctg
    11581 gcgcgccgat agggcttgag ctcggccgcc ggcacgccga agggatgcgc gaagccgccg
    11641 cgggcaatgt cgaggacgtc ggcggcgacg gcgctggccg ttgccttcgc accggccccg
    11701 ggtccggcca acaaaacgtt gccgacaaaa tcgccgtcga tggcgatgca gttggtgacc
    11761 cccgaaacct cggcaatggc cgaatgcttg ggcaccatgg ccgcatgcac gcgctgctcg
    11821 ataccgctgt cggtgaggag cgcgacgccg agcagtttga tgcggtagcc aagatcgtcg
    11881 gcggcctcga tgtccgcctg gctgatgccc tggatgcctt ccacgtggat ctgatcgagc
    11941 gccaccttgg tgccgaaggc gaggctcgtt agcagggcaa gcttgtgggc cgtgtcgaag
    12001 ccgccgatgt cgaaggtggg atcggcttcg gcatagcctt tgtcctgcgc ctctttcagc
    12061 acctcggcaa aggcgcggtg ctcctcctgc atctgggtca ggatgtagtt gcaggtgccg
    12121 ttcagaatac catagacgcg gcggatctga ttgccggcaa gcgcctcgcg cagcgtcttg
    12181 atgaccggga tgccgcccgc cacggccgcc tcgaacttga gggccacggc gcgcgcttcc
    12241 gccaagctcg cgagctccgt gccgtgcttg gccaacaggg ccttgttggc cgtcaccacg
    12301 tgcttgccgg ccttcagcgc ggcctcgacg gcggcgcgcg ccacgccctc ctcgccgccg
    12361 atcagctcga tgaacacatc gatgccggga tcggtcgcca ggcgctcggc ctcttcgaac
    12421 caggcaacgc cctcgagcgt cacggagcgc tttttctggc gcgagcgggc actgaccccg
    12481 gcgatggtga tctcgcgccc cgaggtcagc gccaagcggg cgccgtgctc ctggatgagt
    12541 tgcaccaatc cagcaccgac ggtgccaagg ccagcgatgc caagcttcaa cggttggctc
    12601 atgcgaacca agattaacgc gatgcctttt gcggaatagg gatcacgtta tgcatggttt
    12661 cgggggcgcg cgcgagaaac tttttgatgt t
//
LOCUS       DASTMT010000229         1661 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_4059760, whole genome shotgun sequence.
ACCESSION   DASTMT010000229 DASTMT010000000
VERSION     DASTMT010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 1661)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1661)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1661
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4059760"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..387)
                     /locus_tag="VFR00_05420"
     CDS             complement(<1..387)
                     /locus_tag="VFR00_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006312640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HEU0158726.1"
                     /translation="MSDLSDLTTLTLVEARAGLAQRRFSATELTQAFLDAIEASNKSL
                     NAFVLPTPERALASAKLSDERLASGQARPLEGLPLGIKDLYCTKGVRTTACSHILAGF
                     TPTYESTVTQNLWDAGALMLGKLNNDE"
     gene            complement(384..860)
                     /locus_tag="VFR00_05425"
     CDS             complement(384..860)
                     /locus_tag="VFR00_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007878661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU0158727.1"
                     /translation="MPITVTVETPLSADVRRLIGELNATLLALTPAEFCSHLSVEEMA
                     APHTTVFVARDEGAAVACGALRRHADGIGEVKRMYTEPAHQGRGIGARILGEIEGLAR
                     REGLMRLVLETGHRHPAAWRLYERAGFGRCPPVLDYPPSPYSVFYAKSMPRADVPA"
     gene            complement(863..1150)
                     /gene="gatC"
                     /locus_tag="VFR00_05430"
     CDS             complement(863..1150)
                     /gene="gatC"
                     /locus_tag="VFR00_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006932863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEU0158728.1"
                     /translation="MEVDEATVRRIARLARIKVTEQEAKALETELSGIFEWIKQLDEV
                     DTRAVEPMTRVVAMTLKRRADEITDGGIADDIVANAPAAADHFFVVPKVVE"
     gene            1252..>1661
                     /locus_tag="VFR00_05435"
     CDS             1252..>1661
                     /locus_tag="VFR00_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018699713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HEU0158729.1"
                     /translation="MPALKPAAQRVQDALAAKGLEASVRHMPQATRSAAEAAAACGCS
                     LGQIVKSLLFRGATSGKPYLLLVSGANRVNEQGVAAVIGEALRRPDADDVREVTGFSI
                     GGIPPLGHRTPLITLMDAALLDYDLVWAAAGTPDS"
BASE COUNT          258 a          572 c          546 g          285 t
ORIGIN      
        1 ctcatcgttg ttgagcttgc ccagcatcaa ggcgccggcg tcccataggt tctgcgtaac
       61 cgtcgactca taggtcggcg tgaagccggc cagaatgtgg gagcaggcgg tggtgcgcac
      121 gcccttggtg caatagaggt ccttgatgcc gagcggcagc ccttcgaggg gtcgtgcttg
      181 cccgctcgcc aagcgctcat cgctgagctt ggcactcgcc aaggcccgct ctggtgtcgg
      241 cagcacgaac gcattgagcg atttgttgct ggcctcgatg gcgtccaaaa aagcctgcgt
      301 caattccgtg gccgagaaac gccgctgcgc caggccggcg cgggcttcaa ccaaggtcag
      361 tgtcgtgagg tcgctgagat cgctcatgct gggacgtccg cgcgcggcat gcttttggca
      421 tagaacaccg agtaggggga cgggggatag tcgagcacag gcgggcagcg gccgaagccg
      481 gcgcgttcgt aaagccgcca ggccgcgggg tggcgatggc ccgtctccag caccaggcgc
      541 attaaaccct cgcgccgggc caagccctcg atttctccca gaatgcgcgc gccgatgccc
      601 cggccctgat gcgcgggctc ggtatacatg cgtttgactt cgccgatgcc gtccgcgtgc
      661 cggcgcaagg cgccgcaggc gaccgcggca ccctcatcgc gcgccacgaa caccgtggta
      721 tgcggggccg ccatctcctc gacgctgagg tgggagcaga attcggccgg cgtcagggcc
      781 agcagggtgg cattgagctc gccgatcagg cggcgcacgt cagcgctcag cggagtttcg
      841 acggtcacgg tgatcggcat gactactcca ccaccttcgg caccacgaag aagtgatcgg
      901 ccgcggccgg ggcattggcg acgatgtcat cggcgatgcc cccgtcggtg atttcgtccg
      961 cccgcctctt cagggtcatg gcgacgacgc gggtcatggg ctcgaccgcg cgggtatcga
     1021 cttcatcgag ttgcttgatc cactcgaaga tgcccgatag ctcggtctcc agcgccttgg
     1081 cctcctgctc ggtgaccttg atgcgtgcca agcgcgcaat acgccgcacg gttgcttcgt
     1141 cgacttccat tgcctcgtcc gcgctcatat tcgcgcctga ttgcgctgca ctcataacgg
     1201 cgctaaaggc cggctgcaac cgcagctcga cccgcccacc ggaggcttcc catgcccgcc
     1261 ctcaaacccg ccgcccagcg cgtgcaggac gcgctcgccg ccaaggggct cgaggcgagc
     1321 gtgcggcaca tgccccaggc cacgcgcagc gccgcggagg ccgccgccgc ctgcggctgc
     1381 agcctcggcc agatcgtcaa atcgctgctc tttcgcggcg ccacctcggg caagccttat
     1441 ctgttgctgg tctccggcgc caaccgcgtc aacgaacagg gcgtcgccgc ggtgatcggg
     1501 gaggctttgc ggcggccgga cgccgatgac gtgcgcgagg tgaccggctt ttccatcggc
     1561 ggcattccgc ccttggggca ccgcacgccg ctgatcacct tgatggacgc agcgctgctc
     1621 gactacgacc tcgtctgggc cgccgccggc acccccgatt c
//
LOCUS       DASTMT010000230         1948 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_453381, whole genome shotgun sequence.
ACCESSION   DASTMT010000230 DASTMT010000000
VERSION     DASTMT010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 1948)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1948)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1948
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_453381"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..146)
                     /locus_tag="VFR00_05440"
     CDS             complement(<1..146)
                     /locus_tag="VFR00_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011090330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEU0158730.1"
                     /translation="MNRITGRSAFLALLKDEGITHLFGNPGTTELPIMHALKDHPDLT
                     YVLGL"
     gene            504..986
                     /locus_tag="VFR00_05445"
     CDS             504..986
                     /locus_tag="VFR00_05445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0158731.1"
                     /translation="MLRARLLPARLLRAGLFAGGVMPLRLGATWLLCLLGGLPPAAAD
                     PTGYSCSFAAGTAHAYDKGQFVAEQPAPLSFSIGAIDGAAQSAEVRTERGTGTLRLVQ
                     AVNATHFLEVVTEGYLNVTTVYDKDEATGKYPAVHSRHLAILGQPVVTQYQGFCEAKN
                     "
     gene            1052..>1948
                     /locus_tag="VFR00_05450"
     CDS             1052..>1948
                     /locus_tag="VFR00_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003500304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HEU0158732.1"
                     /translation="MLKKLLIANRGEIALRIMRTAHRLGVACVAVCSEADRAAPFVAL
                     ADEIVDIGPGPASESYLRIDKILAAAKTTGADAIHPGYGFLAENAEFAEAVAAAGLRF
                     VGPSPQAMRRMGGKAEAKGIASAAGVPVIPGYQGANQTPATLAKEAKRIGYPVMIKAV
                     AGGGGRGMRLVEREADFAAALDGARREAAAAFGDDRVLIEKLLLAPRHIEVQVFGDSH
                     GEVVHLFERDCSLQRRHQKVVEEAPAPGMSRELRQAITTAAVACAKAVAYEGAGTVEF
                     LVEGGALSPAAPWYFIEMNTRLQ"
BASE COUNT          298 a          620 c          692 g          338 t
ORIGIN      
        1 agcccgagca cgtaggtgag atcgggatga tccttgaggg cgtgcatgat cggcagctcg
       61 gtagtgcccg gattgccgaa caaatgcgtg atgccctcgt ctttgaggag agcgagaaag
      121 gcggagcggc cggtgatgcg gttcatgtgc tggggtacat acctgtgcgc ttggcggtcg
      181 ggcgaagtgc gagcctaacg cggtcccgcc tcgatcgaca aggttttcgc ccggcgatcg
      241 gcgccatcgc gcaccccagg ggcgatttgc cggttgcaca ttggcggcgg ccattcttct
      301 atgtctgccc ttgctgttgc cggccggttc cctgccagcc ggggctgacc tgcacgctgg
      361 tgccgggctc atcgctgcgg acacctgttg ccgggacggt tgttgcgagc ccagtcgttg
      421 cgaggacagt attgcgagga cgcttggtgc aaggacgctt attgcgaggt caggtgttgc
      481 gaggtcgggt gttgcgaggt caggtgttgc gagcacgctt gttgccggca cgtttgttgc
      541 gcgcgggcct gttcgccggc ggcgtcatgc ccctgcgtct cggcgcaaca tggctgctat
      601 gcctgctcgg cggcctgccg cccgcggcgg ccgaccccac gggctacagc tgcagtttcg
      661 ccgcgggtac ggcgcatgcc tacgacaagg gccagttcgt cgcggaacag ccggcgcccc
      721 tgagcttcag catcggcgcc atcgacggcg ccgcgcagag cgcggaggtg cgcacggagc
      781 ggggtacggg aaccttgcgc ctggtgcagg cggtgaacgc aacgcacttt ctggaggtgg
      841 tgacggaggg ctatttgaat gtcacgacgg tctacgacaa ggacgaggcg accggcaaat
      901 atcccgccgt gcattcgcgg catctagcca tcctgggcca gccggtcgtc acgcaatatc
      961 agggcttctg cgaggccaag aactaacccg ggcctggcgc cgggacgggg ttgccccgtg
     1021 ccgcgcccag atgccataag cttccctctc catgctgaaa aagctcctga tcgccaatcg
     1081 tggcgaaatc gctctccgca tcatgcgcac cgcgcaccgc ttgggagtgg cttgtgtcgc
     1141 cgtttgctcg gaggccgatc gcgccgcccc gttcgtggcg ctggccgatg agatcgtcga
     1201 cattggtccc ggtccggcga gcgaaagcta tctgcgcatc gacaagatcc ttgccgctgc
     1261 caagacgacg ggggccgacg ccatccaccc aggctacggc tttctcgccg agaatgccga
     1321 gttcgccgag gccgtggcgg ccgccggcct gcgcttcgtt ggtccctcgc cccaggccat
     1381 gcggcgtatg ggcggcaagg ccgaggccaa aggcatcgcc agcgcggccg gcgtgccggt
     1441 gataccgggc tatcagggcg ccaaccaaac gccggccacg ctggccaagg aggccaagcg
     1501 catcggctat cccgtcatga tcaaggccgt cgccggcggc ggcgggcgcg gcatgcgtct
     1561 ggtcgagcgg gaagccgact tcgccgcggc gctcgacggt gcccgtcgcg aggccgcggc
     1621 cgccttcggc gacgaccgcg tgctgatcga aaaactgctc ctggcgccgc gccatatcga
     1681 ggtgcaggtg ttcggcgaca gccatggcga agtggtgcac ctgttcgaac gcgactgctc
     1741 gttgcagcgt cgacatcaga aggtggtgga agaggcgccc gcgcccggca tgtcgcgcga
     1801 gttgcgccag gcgatcacca cggcggcggt ggcctgcgcc aaggcggtgg cctacgaggg
     1861 cgcgggcacc gtcgagttcc tcgtcgaggg gggtgcgctt tcgccggcgg cgccttggta
     1921 cttcatcgaa atgaacacgc gcctgcag
//
LOCUS       DASTMT010000231         1940 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_4060344, whole genome shotgun sequence.
ACCESSION   DASTMT010000231 DASTMT010000000
VERSION     DASTMT010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 1940)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1940)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1940
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4060344"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(518..760)
                     /locus_tag="VFR00_05455"
     CDS             complement(518..760)
                     /locus_tag="VFR00_05455"
                     /inference="COORDINATES: protein motif:HMM:NF018294.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1127 domain-containing protein"
                     /protein_id="HEU0158733.1"
                     /translation="MGTTRAISQSIPPRSAFAKMILRSGLVKFRHWRRSRRAERELLA
                     LDDRLLKDIGICRADIPDAVRGQARNFYTLASDQNL"
     gene            complement(1117..1389)
                     /locus_tag="VFR00_05460"
     CDS             complement(1117..1389)
                     /locus_tag="VFR00_05460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0158734.1"
                     /translation="MKPSLAVTIFACADCNLRGFSFKENGSDLPPRADGGLWIAIASV
                     PMSFVELEKYTSDPAAVLANLREHGCHVAPATAKILTFPAPHRSSA"
BASE COUNT          332 a          580 c          671 g          357 t
ORIGIN      
        1 gggggcggtg gcgcactggc agcctttggc tggccgggct cgcagcctgc gagcgccaag
       61 ccgagcagca ccacgctggc gctgacgccc aaggccttga gcgacgggat ctggatcata
      121 ggatttccgg gacatgagag ggcgatgcgt gcctgcatat ggtatccagg tcgggtgtgt
      181 gcagaccggc tcgcgaggac gtgaaggcgc gcgaggcagg cacttccgag cgcgacatgg
      241 ccgcagcgaa aagaacctga tgcaggagcc caacaaagga gaacggcttg gcgcatctct
      301 cgggcgaagc aacggtatct tttcatcgct gccgccgttt gcgcatgtca gcggcagcgg
      361 gacaaacggt taatcggtga ggccgtgccg actcgcggcc tagcgccaag tctatagagc
      421 cacaggctgg ggccaacgac gtgcgtatcg gtgatctcga tgagccgaga cccgcaccgc
      481 caggccgcag ggcggcctgg ccggggtggt ttcgcgctta taagttctgg tcggaggcca
      541 gcgtatagaa attccgcgct tggccgcgca cggcatcggg aatgtctgcg cggcagatcc
      601 cgatgtcctt gagcaaccga tcgtcgagcg ccagaagttc gcgctcggcc cggcggctcc
      661 gccgccaatg tctaaactta acgagcccgc tgcgcaggat catcttcgca aaggcgctgc
      721 ggggcggtat cgactggctt atggctcttg tagttcccac acttagctcc atttcctatt
      781 tccaagtccg tgggcggcga tgctggcgac gggcgatttg tttgttttga cccggcgcac
      841 tgacgcgctc gccgcatggt ttgtcctcct tgatccgatt ggctgatttt caaagttact
      901 gatgtgttct ttttcgcggc ggcgaaaaga acgcctcacg taggggtgat gaggtttgat
      961 ccgtttgccc acagcaaacc gcccgtcgaa gtgcgggggt gacgtcgggg ttttgcccgg
     1021 ggtcgggcgg cgcgcacggg atgcgtgtcc gtgtaaaggc gccttattct tttggggcag
     1081 cacggccggg cgaccggcag ggagcccccg cgccttctag gcgctgctgc ggtgcggggc
     1141 tggaaaggtg aggatcttgg cggtggcggg cgcgacgtga cagccgtgct cccttaggtt
     1201 tgccagcacg gcggccggat cggacgtgta cttttcgagt tcgacgaaac tcatgggcac
     1261 gcttgcaatc gcgatccaaa gcccaccatc ggcacgcggc ggcagatccg acccgttctc
     1321 tttgaaagag aagccgcgca ggttgcagtc ggcgcaggca aagatggtga cggcaagcga
     1381 cggtttcatg cgcgtatctc ggctgagagg acaagagcca tcgagcgccg gcccggcgcg
     1441 ctcagcccgc gggtgggcgc accgaccacg ccgcgcgcgt gcgctgcccc aggcttggcc
     1501 gcgtaacgag ctcttgtggg aaagacaagc gatatatagc gctgacgccc tggctggcgt
     1561 agagggtgcg gcgaagcaaa gcttgagcgg tgccggtcct tggcttcttt atggccgctg
     1621 attccaggcc ccgtgccaag catcaacggc gcgctccatg gtgctgggct gccggcgtct
     1681 cacgcctcat cgaggctgat cccgccgtcg tggctaagga cattgcgggc ggagttgaag
     1741 agagcgcttc atcgccgagc cgccgcgtca ccgccggcaa tgggaccatg ggcatcactc
     1801 cggctggaaa aggcgagctc gcattcgtca gcctggttgc gcttgccgag gagaaaatcg
     1861 cctggggcca acggctgcgc gccttgcggg gcatctggcg gcgagcgccc ggcctccctg
     1921 agcgcacgtc cagtacgccc
//
LOCUS       DASTMT010000232         8091 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_1110920, whole genome shotgun sequence.
ACCESSION   DASTMT010000232 DASTMT010000000
VERSION     DASTMT010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 8091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8091
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1110920"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..486
                     /locus_tag="VFR00_05465"
     CDS             <1..486
                     /locus_tag="VFR00_05465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0158735.1"
                     /translation="HASVAQLLEVRKRLADIYAAEGLERGRVAIAPLRHVCITNAKAE
                     AQDFIDNVRHQIRLSQSLRFREELIDGVMLVEKPYRNEPPSEELAKHVLVSDAETVAE
                     RLAALVRAAQPRHMLLHFQAGASPQRTALKSIQAFAERVRPMLETELGPLERLGSEQA
                     A"
     gene            complement(566..1792)
                     /locus_tag="VFR00_05470"
     CDS             complement(566..1792)
                     /locus_tag="VFR00_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007604171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEU0158736.1"
                     /translation="MKTLYRRCAGLDVHKKEVVACLRLVVRGKASYEVRRFPTTTGSL
                     IELADWLEQAGCTHVAMEATGVYWKPVWHILEGRFQQILANAAHIKGVPGRKSDMNDA
                     TWIADLLAHGLIRASFVPPQPIQELRDLTRTRKQLTREIVQHTQRIQAVLEEANVKLS
                     SVITEILGASGRRILKAIIAGETDPQKLSELGGPRLATTRVELADALHGRIRTHHRFL
                     IGQHLKTIEQLEETIAAFDARIEAALAPFRDTLERLKEVPGLSETSAQILLAEVGTDM
                     RQFPTAGHLLSWAGLIPRLDESAGKRRSTRVRKGAPWLKPVLVQSAWAASRKKNSYFQ
                     AQFLRLKARCGPKKAVVAVAASILTTAYHMLADGTCYQDLGADHFARRDPARAAAKLA
                     NRIRNLGFNVEIRPAA"
     gene            complement(2040..3245)
                     /locus_tag="VFR00_05475"
     CDS             complement(2040..3245)
                     /locus_tag="VFR00_05475"
                     /inference="COORDINATES: protein motif:HMM:TIGR00200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family nicotinamide mononucleotide
                     deamidase-related protein"
                     /protein_id="HEU0158737.1"
                     /translation="MRCEVIAIGTELLLGIIVDSNSAWIGEQLALSGIDSYFQVKVGD
                     NFGRIEASIRQALARSDAVICCGGLGPTQDDITRDVIAQVMGVGLARDAAIADKIRRI
                     FEARGRVMSQNNLRQADVPVGAQPIAQMPGTAPGLVCPIGDKVIYAVPGVPSEMREMM
                     LGTILPDLRRRAGQSAVIRSRVLRTWGMSESGLAEVLADRMAELDRLGNVTIAYQASG
                     IDGIKVRLVAKCKDEATAAEVLGAEEALIRTLLGDVIFGVDEESMEVAVLAALRRKSL
                     TLAVAEEVTGGLLAARMTLVDPAMQTFRGARIAGEPVAKSTDGIGARAAAAATTARVA
                     FGTMVGAAAIAGPTRQSTGPLTAPVALAASIAGQEHAEEIVLPADRGRLRDFAVISLL
                     NLLRKALAA"
     gene            complement(3273..3887)
                     /locus_tag="VFR00_05480"
     CDS             complement(3273..3887)
                     /locus_tag="VFR00_05480"
                     /inference="COORDINATES: protein motif:HMM:NF019590.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIPSNAP family protein"
                     /protein_id="HEU0158738.1"
                     /translation="MIFEFRTYTLKPRTLNEFLGRFGEALPRRLALSPLAAFWYTEIG
                     PLNEVIHVWPYANVLERSRIRAEAVKLGIWPPKTQELIDDMRSEIFEPLPFSPPLEPS
                     SHGPYFEMRSYALKPGSLPLMSQRWGEFLPQRLKLSPLIGVFSSDIGGLNQWVHIWAY
                     GSLDQRLEVRRKAAAEGIWPPPGDSPVVRQASKILLAAPYSPIK"
     gene            4097..5299
                     /locus_tag="VFR00_05485"
     CDS             4097..5299
                     /locus_tag="VFR00_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018457846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HEU0158739.1"
                     /translation="MLDVAKPDAARPAAPFDTGRLDRLLEDAGIDVLLATSKHNVQYL
                     LGGHRSIFFDYMDATGITRYLPVLIYPKGAPDKAAYIGHRLESHQKEVAPFWTAEAST
                     STSGSTDATEKAIAYLRKAGLKAKRIGVEMPFLPADAAQTLRAGLPDSELRDAVFVLE
                     RLRAKKRPDELALLRQASELVIDSMLAVITRHPPGTSKRELFEALRREEVNRGMTFEY
                     CLLTAGTSLNRAPSDQRWEMGDIISLDSGGNYHGYIGDLCRMAIQGEPDAELEDLLSE
                     IETIQRSAMAPIKAGAVGSVIYGAATPLVRQSKNHNHLHFLAHGMGLVSHEAPRLAPR
                     GSMAYDPYDADYPLETGMVISVETTLHHPRRGFIKLEDTVAVTDTGFEIYGEGARGWN
                     RGGTAVSA"
     gene            complement(5324..6160)
                     /locus_tag="VFR00_05490"
     CDS             complement(5324..6160)
                     /locus_tag="VFR00_05490"
                     /inference="COORDINATES: protein motif:HMM:NF018352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MipA/OmpV family protein"
                     /protein_id="HEU0158740.1"
                     /translation="MMLSGGAKALLRAGLGAVTVLLALPSAKAQDRSEPFGFAGTPWQ
                     VGGFVFVTPKFEGAKSYDVLGFPFIAPAGFGQGGGSAIDVRGADDVRFRLVARDGFEA
                     GPLAGYRFGRNQDDAARLAGLGDIDGGLVLGAYAGYRAGPWFFSVSYHHQVTGDDDTG
                     GLLRLAIDHTWRLAPNAKLVTSLGTAYASNQYMQTYFGVSAAQAAASLAHLPQFDASA
                     GFKDVSLGATATVELDPRWTLYLTGRYTRLIGDAADSPVIETENGFFGGAGLSYKFDW
                     AR"
     gene            6493..7503
                     /locus_tag="VFR00_05495"
     CDS             6493..7503
                     /locus_tag="VFR00_05495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0158741.1"
                     /translation="MGTGNRRGVDRRRFLASASSLIAVPAAIGAMAATGVAQTAPPAA
                     GLNPYRELAAIAAEAERLGLSVPRISAGVTGGTEFTALMPAIVDFLDNIQASAKSGAA
                     ATAEVEALLDRTSALLQALNRAERSPPDRDPASGAGAATVARPAFEELRQGYVELFQS
                     CSIRDEHVADVNWCVGKLTDSDSREQYNRVAEEVCAPWYFIGIIHAMEASFDFRTHLH
                     NGDPLKARTVQVPSGRPLVWDPPNDWAASAIDAITFDHFADQPDWSLARTLYNWEGYN
                     GWRSRARGINTPYLWSFSNHYSKGKFVADNVWDADAVSKQCGAAVMLRALVNQGLVAL
                     PS"
     gene            complement(7523..8086)
                     /locus_tag="VFR00_05500"
     CDS             complement(7523..8086)
                     /locus_tag="VFR00_05500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ActR/PrrA/RegA family redox response regulator
                     transcription factor"
                     /protein_id="HEU0158742.1"
                     /translation="MVDDLAFKPDELPEDRSLVLVDDDRSFVLRLARAMETRGFEVRT
                     AHSVAEGLELIRQKAPAFAVVDMRLEDGNGLDVIAALSELRPQARTIVLTGYGNIATA
                     VSAVKLGAVDYLAKPADADDVTDALLAPHDEKARPPENPMSADRVRWEHIQRVYELCN
                     RNVSETARRLNMHRRTLQRILAKRAPK"
BASE COUNT         1382 a         2685 c         2680 g         1344 t
ORIGIN      
        1 catgccagcg tcgcccagct gctcgaggtg cgcaagcgcc tggctgatat ctacgccgcc
       61 gagggccttg agcgaggacg cgtggcaatc gcgccgctgc gacatgtatg catcaccaac
      121 gccaaggccg aagcgcaaga cttcattgac aacgtgcgcc atcagatccg cctctcgcaa
      181 agcctgcgct ttcgcgaaga actgatcgat ggcgtcatgc tggtcgagaa accctaccgc
      241 aacgagcccc cgagcgaaga actggccaag cacgtgctcg tgagcgacgc ggaaaccgtg
      301 gccgagcgcc tggccgccct cgtgcgcgcg gcgcaaccca ggcacatgct gctgcacttc
      361 caggccggcg cctcgccgca gcgcacggcc ctcaagtcca tccaagcctt cgccgagcgc
      421 gtgcgaccga tgctggaaac ggagctgggt ccgctcgagc ggctcggcag cgaacaggcg
      481 gcctgaggcc ggccagcaac gcgcggcatc gcgcgcgggc tgacgcccgg cgcgatcgcc
      541 tatccagaaa ccacgtcggg ttgtgtcatg cggctggcct gatctcaacg ttgaagccga
      601 ggttgcggat gcggttggcg agcttggctg cggcacgggc tgggtcacgg cgggcgaagt
      661 gatcagcacc gagatcctgg tagcacgtac cgtcggccag catgtggtag gcggtggtga
      721 ggatcgaggc cgccacggcg acaacagcct ttttgggccc gcaccgtgcc ttgagccgga
      781 ggaactgagc ttgaaagtag ctgtttttct tgcgggatgc agcccaggca ctctgaacga
      841 gcactggctt tagccatggg gcgccttttc tgacgcgggt cgagcgtcgc ttgccggcgc
      901 tctcgtcgag gcggggaata agacccgccc aggataggag atgaccagca gttgggaact
      961 ggcgcatgtc cgtgccgacc tcggcgagga ggatctgtgc cgaggtctcg cttaaacccg
     1021 gaacctcctt caatcgttcg agggtgtcac gaaagggcgc gagcgcggcc tcgatccgcg
     1081 cgtcgaaggc tgcgatcgtc tcttccaact gctcaatggt ctttaaatgc tggccgatga
     1141 gaaagcgatg atgggtgcga atacggccgt gcaaggcgtc cgcaagctct acgcgcgttg
     1201 ttgccagacg ggggccaccc agttcggata gcttttgagg gtccgtctcg ccagcgatga
     1261 ttgccttcaa aatgcgccgt ccgcttgccc ccaagatctc cgtgatgacc gaggatagct
     1321 tgacgttggc ttcctcgagt acggcctgga tgcgctgagt atgctgcacg atctcgcggg
     1381 tgagttgttt cctcgtccgc gtcagatccc gcagttcctg gatgggctgt ggcggcacga
     1441 agctcgcccg gatcaggcca tgcgccagca ggtcggcgat ccaggtcgca tcgttcatgt
     1501 cgctcttgcg tccgggtacc cctttgatgt gcgcggcgtt ggccaaaatc tgttggaacc
     1561 gcccttccag gatatgccag accggcttcc agtagacacc tgtggcctcc atggccacat
     1621 gcgtgcaccc ggcctgctcc agccaatccg cgagctcgat gagcgacccc gtggtggttg
     1681 gaaaccggcg cacctcgtaa cttgccttgc ctcggacgac gagccgaagg caggccacaa
     1741 cctccttctt gtgcacgtcg agacccgcgc accgtctgta caatgtcttc atcgcgccct
     1801 cctctaggcg cggtcggcgt gaggcccttg ctcaaatgaa ctctatccac cgtgctcccg
     1861 aacggacgcc agccatccag gctgacgctc gccgtagcaa caatcacggg tgctcgaagg
     1921 gcctccagtc aaactcaggc acgggctcga tagcaccaag gcgacgccga cttcgttgcc
     1981 gaccgcgctc taggatacta ccgtttcata aatccgggtg cgcaaacgcg aatggcaact
     2041 caggccgcca aggctttgcg caagagattg agaaggctga tcacggcaaa atcgcgcaag
     2101 cggccgcggt cggcgggaag gacgatctct tcggcgtgct cctgaccagc gatgctggcg
     2161 gccagcgcaa ccggcgccgt cagaggccct gtgctctgac gggttgggcc ggcgatggcg
     2221 gcggcgccga ccatggtgcc aaaggcgacg cgggccgtcg tcgcggccgc ggccgcccgc
     2281 gcaccaatcc cgtccgtgct cttcgccacg ggctcgcccg caatgcgcgc accccggaag
     2341 gtctgcatcg ccgggtcgac cagcgtcatg cgtgcggcca gcaggccccc cgtcacctct
     2401 tcggcgaccg caagggtcag ggacttacgg cgcagcgccg cgagcacggc cacctccatg
     2461 gactcctcgt cgacgccaaa gatcacgtcg cccaacagcg tgcggatcag ggcttcctcg
     2521 gcgccgagca cctcggctgc ggttgcctcg tccttgcact tggccaccag gcgaaccttg
     2581 atgccgtcga tgccgctggc ctggtaggcg atcgtcacat tgccgagccg atcgagttcc
     2641 gccatgcggt cggccagcac ctcggccaga cccgattcgc tcatgcccca ggtgcgcagc
     2701 acgcggctgc ggatcaccgc cgactggccg gcccggcgcc tgaggtcggg caggatggtg
     2761 ccgagcatca tctcgcgcat ttcactgggc acgcccggca ccgcgtaaat gaccttgtca
     2821 ccgattggac aaaccagccc cggtgccgtc ccgggcattt gcgcgatcgg ctgggcgccg
     2881 acgggcacgt ccgcctggcg cagattgttt tgcgacatca cccggccgcg cgcctcgaaa
     2941 atgcgccgga tcttgtcggc gatcgccgca tcgcgcgcga ggcccacgcc catgacttgg
     3001 gcgatcacgt cgcgggtaat gtcgtcctgc gtcggcccga gcccgccgca gcagatcacg
     3061 gcatcactgc gcgccagggc ctggcggatc gaggcctcga tgcgcccgaa gttgtcgccg
     3121 actttgactt ggaagtagga gtcgatgccg ctgagcgcca attgctcgcc gatccaggcc
     3181 gagttgctat cgacgatgat gccgagcaga agctcggtgc cgatggcaat gacttcgcac
     3241 cgcatcgctc gcctcgtgtg tgcgggggtt gactacttga tgggcgaata cggtgccgcc
     3301 agcaggattt tggaggcctg gcgcacgacg gggctgtcgc ccggcggcgg ccaaatcccc
     3361 tcggcggcgg ccttccggcg cacctcgagg cgctgatcga ggctgccgta ggcccagatg
     3421 tgcacccact ggttgagccc gccgatgtcg gagctgaaga cgccgatcaa cggcgacagc
     3481 ttgaggcgct gcggcaggaa ctcgccccag cgctggctca tcagcggcag gctgccgggc
     3541 ttcagggcat agctgcgcat ttcgaagtag ggaccgtggc tggagggctc gagcggcggc
     3601 gagaagggca ggggctcgaa gatctccgag cgcatgtcgt cgatcagctc ttgcgttttc
     3661 ggcggccaga ttcccagctt cacggcttcg gcacggatgc gcgagcgctc gagcacgttc
     3721 gcgtacggcc agacatggat gacctcgttg aggggaccga tttccgtgta ccagaaggcg
     3781 gcgagcggcg agagcgccag acgccggggc aaggcctcgc cgaagcggcc caagaactca
     3841 ttgagcgtgc gcggcttcag cgtataggtg cgaaactcga agatcatcgg cgcgaaccct
     3901 ccctggcggc tgcccagcag gcaaggtctg cccgtgccgc tgccgcttgg gtatgcacct
     3961 tcccaatcta gccggcaagt cggccgcgcc cccaggcaag gcgtctcccg cccgatcgcg
     4021 gcgtctcttg cctttcgaag cggcgatcgg cgagctatga tttggccgcg agtaagccgc
     4081 caacggagcc gatcccatgc tcgacgtcgc caaaccggac gccgcccgac ccgccgcacc
     4141 cttcgacacg ggccgccttg atcggttgct cgaggacgcc ggcatcgatg tgttgctggc
     4201 gacctccaag cacaacgtgc agtacctgct gggagggcac cgctcgatct tcttcgacta
     4261 catggatgcc acgggcatca cgcgctacct gccggttctg atttatccca agggcgcccc
     4321 cgacaaggcc gcctatatcg ggcatcgcct cgagagccac caaaaggagg tggcgccatt
     4381 ttggaccgcg gaagcctcca cgtcgacctc cggatccacc gatgccaccg aaaaagccat
     4441 cgcctacctg cgcaaggcgg ggctcaaggc caagcgcatc ggcgtcgaga tgccctttct
     4501 gcccgccgat gccgctcaaa ccttgcgcgc cggcctgccc gacagcgaac tccgcgatgc
     4561 cgtgttcgtg ctcgagcgcc tacgcgcgaa aaagcgcccc gacgagctgg cgctcctgcg
     4621 ccaggcctcc gagctcgtga ttgacagcat gctggccgtg attacgcgtc accctcccgg
     4681 caccagcaag cgcgagctgt tcgaggcctt gcgccgcgag gaggtcaatc gcggcatgac
     4741 gttcgagtat tgtctgctga cggccggcac gagcctcaac cgcgcgccct ccgaccagcg
     4801 ctgggagatg ggcgacatta tctcgctcga ttccggcggc aactaccatg gctatatcgg
     4861 cgacctctgc cgcatggcga tccagggcga gcccgacgcc gagctggaag acctgttgag
     4921 cgagatcgag accattcagc gcagcgccat ggcgccgatc aaggccgggg ccgtgggttc
     4981 ggtaatctat ggggcggcaa ccccgctcgt gcgccaatcc aaaaaccaca accatttgca
     5041 tttcctggcg cacgggatgg gtctcgtcag tcatgaagcg ccgcgcctgg cgccgcgcgg
     5101 cagcatggct tacgaccctt acgatgccga ctacccgctc gaaaccggca tggtgatttc
     5161 ggtggaaacg acgctgcacc acccccggcg cggcttcatc aagctggaag ataccgtcgc
     5221 ggtcaccgac acgggcttcg agatctatgg cgaaggcgcg cgcggttgga accggggtgg
     5281 gaccgcggtc tccgcctgag acggcgctgc ggcgcgcgtt gctctagcgc gcccagtcga
     5341 acttgtagga caagcccgcg ccgccgaaga agccgttttc ggtctcgatc accggactgt
     5401 cggcggcatc gccgataagg cgcgtgtagc gccctgtcag ataaagcgtc cagcgcgggt
     5461 cgagctcgac ggtggccgtg gcgccgaggc tcacgtcctt gaagccggcg gatgcatcaa
     5521 attgcggcag gtgggcaagc gaggccgccg cttgcgcggc actgaccccg aagtaggtct
     5581 gcatatactg gttcgaggca taggcggtcc ccaggctcgt caccagcttg gcgttgggtg
     5641 caaggcgcca ggtgtggtcg atggcgagcc gcagcaagcc cccggtgtca tcgtcgccgg
     5701 tcacctgatg gtggtaggag acggagaaga accaaggccc ggcgcgatag cccgcgtagg
     5761 cgccgagtac gagcccgccg tcgatgtcgc cgagcccggc caagcgggcg gcgtcgtcct
     5821 gattgcgccc gaagcggtag ccggccagcg ggccggcctc gaagccgtcg cgggcaacga
     5881 gacgaaagcg cacgtcatcg gcgccgcgga cgtcgatggc gctgccgccg ccctggccga
     5941 agccggccgg ggcaataaag ggaaagccca acacgtcgta ggatttggcg ccttcgaact
     6001 tcggcgtcac gaagacgaag ccgccgacct gccagggtgt gccggcaaaa ccgaaaggct
     6061 cggaacggtc ctgcgccttg gccgagggga gggcgaggag gacggttacc gccccgaggc
     6121 ctgctcggag aagggctttg gctccgccac tcagcatcat ggtcatcccg ttgcggtcac
     6181 gcgcgcctgt gctcgattag tgtagcggat taggcttacc caaagcctag gcaatcggcg
     6241 gcttcgcccg gatccccagt gactgttgcc gcgacgacac ggctgccgcg gcgaggtccc
     6301 tctcgccgcg gcggcgaggc aaccgcgtcg gcggtggcgg agccgtggcg cgccggtcgg
     6361 ccttcgacta gacttggggg gcgcggcacg cgccaagtgc cagatctcgg cagggcaggc
     6421 gcgtttcggg tgtgcttcgg cgcaccgctg gtggctacgg cgggcgcggt gagacgcaaa
     6481 cagggggtta gcatggggac cggcaaccgg cggggcgtgg atcgccgccg ctttcttgca
     6541 agcgcctcct cacttatcgc cgtgccggcg gcgatcggcg ccatggccgc cacgggcgtg
     6601 gcccagacgg cccccccggc ggccggcctc aatccttacc gcgaacttgc cgcaatcgcg
     6661 gccgaggccg agcggcttgg gctttcggtg ccgagaattt ctgcgggcgt gaccggcggc
     6721 acggagttca ccgcgctgat gccggccatc gtcgatttcc tggacaacat ccaagcctcg
     6781 gccaagtcgg gggcggcggc cacggccgag gtcgaagccc tgctcgatcg gacctcggcg
     6841 cttttgcagg cgctcaaccg cgccgagcgc agcccgcccg atcgcgatcc tgcctccggt
     6901 gccggcgccg ccaccgtggc gcggccggct ttcgaggagc tgcgccaggg ctacgtcgag
     6961 ctgtttcaga gctgcagcat tcgcgacgag cacgttgccg atgtcaactg gtgtgtcggc
     7021 aagctcacgg atagcgacag ccgcgagcag tacaatcggg tggccgagga ggtctgcgcg
     7081 ccttggtact tcatcggcat catccatgcc atggaagcga gttttgattt tcgcacccat
     7141 ctgcacaatg gcgacccgct gaaagcccga acggtgcagg ttccgagcgg tcgtccgctc
     7201 gtctgggacc cgcccaacga ctgggccgcg agcgccatcg acgccatcac ttttgatcat
     7261 ttcgccgatc agcccgattg gagcctcgcc cgcaccctct acaattggga gggctacaac
     7321 gggtggcgca gccgggcgag gggcatcaat acgccctatc tgtggagctt ctccaaccac
     7381 tacagcaagg gcaagttcgt ggccgacaac gtctgggacg ccgatgcggt gtccaagcaa
     7441 tgcggcgccg ccgtgatgct gagggcgctc gtcaatcaag gcctggtggc gctaccgagc
     7501 tgagctctcg cgcggccgct cttcacttgg gtgcgcgctt ggcgagaatg cgctgcaggg
     7561 tgcgccgatg catgttgagc cggcgcgcgg tttccgagac gttgcggttg cacagctcat
     7621 aaacgcgttg gatatgctcc cagcgcacac gatcggccga catcgggttc tccggcgggc
     7681 gcgccttttc gtcgtgcggg gccagcagcg catcggtcac gtcgtcggca tcggcgggct
     7741 tggccagata gtcgacggca ccgagcttga ccgccgacac cgcggtggcg atgttgccgt
     7801 agccggtaag aacgatggtg cgcgcctgcg gccgcagttc cgagagcgcg gcgatcacgt
     7861 caaggccatt gccgtcctcg agccgcatat cgaccaccgc gaaggcgggc gccttctggc
     7921 ggatcagctc caggccctcg gccacggagt gcgccgtgcg cacctcaaag ccgcgcgttt
     7981 ccatggcgcg ggccagccgc agcacaaagg agcggtcatc gtcgacaagg accaaggagc
     8041 gatcctcggg caactcgtcc ggcttgaatg cgagatcgtc gaccactgct c
//
LOCUS       DASTMT010000233         2620 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_3078283, whole genome shotgun sequence.
ACCESSION   DASTMT010000233 DASTMT010000000
VERSION     DASTMT010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2620)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2620)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2620
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3078283"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..529)
                     /locus_tag="VFR00_05505"
     CDS             complement(<1..529)
                     /locus_tag="VFR00_05505"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU0158743.1"
                     /translation="MRNRKRVFYNSHGDQALYAMIRAAAGEDVELMTLEADDDGERKQ
                     KLAEADAVIVAATRFSEDLLAAAPRLAFVHHQGVGYHDTVAWQALAKRQIPLALTPGG
                     TAESVAEQTIMFMLSVLRMLPYADSELRQGRFHVNAIRPVTRDLSGRTVGILGLGRIG
                     KAVARRLKPFDVKVIY"
     gene            692..1438
                     /locus_tag="VFR00_05510"
     CDS             692..1438
                     /locus_tag="VFR00_05510"
                     /inference="COORDINATES: protein motif:HMM:NF036686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp/Glu racemase"
                     /protein_id="HEU0158744.1"
                     /translation="MPDVLGWRAKFGVLAPSTNTVVEPDFADLRPAGVTNHLSRIFTP
                     NVKGLSNETFRGGVATISGNVMDALKSVMTCEPDHLVMGMSGITFLGGMAGADAFVRQ
                     VEAESGIGISIGSHAAAAALRAYGGVSRIAILSPYWPVMNEEVRRYFTEAGFEVVRDH
                     AMQVTSWVGIAAVTPAQCRSALERLDGDDVDAIVQVGTNLSMLRLAAAAELWLGKPVL
                     AINAATYWHALRSNGMREPIAGFGRLFEAF"
     gene            1504..>2620
                     /locus_tag="VFR00_05515"
     CDS             1504..>2620
                     /locus_tag="VFR00_05515"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016842291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEU0158745.1"
                     /translation="MAEQGNPAPGAPAPDFDVAIVGAGFAGMYMLHRLRSLGFRVRVF
                     EAGGDVGGTWYWNRYPGARCDVESIQYSYQFDPALEQEWDWSERYAAQPELLRYARHV
                     AKRFDLNRDIAFNTRITQASFEATAGVWVLASDRGRFTARFCVMATGCLSSPNLPALP
                     GLESFQGERYHTGQWPHEEVDFTGKRVAVIGTGSSAVQSIPIIARQAKHLYVFQRTPN
                     YAVPAHNRPLAAEELRKAKADYPAMRRRAKQTMTGIDFDYSDKKALLTPAPERQAEFE
                     RRWVHGGLWFLGAYQDLMVDQQANDTAADFVRSKIRARVADPELARRLAPQNTIGCKR
                     LCVDIGYYETFNRDNVTLVDVSDAPIEAITPAGLKAGG"
BASE COUNT          444 a          882 c          855 g          439 t
ORIGIN      
        1 ggtagatgac cttgacgtcg aagggcttga ggcggcgcgc caccgccttg ccgatacgtc
       61 cgaggcccag gatgccgacc gtgcggccgc tcaagtcgcg cgtgacgggc cgaatggcgt
      121 tgacgtggaa gcgcccctgg cgcaactcgc tgtcggcgta gggcagcatc ctcaagaccg
      181 acagcatgaa catgatggtc tgctcggcca cgctctctgc cgtgccaccc ggtgttaggg
      241 ccaacgggat ttgccgcttg gccagggcct gccaggccac ggtgtcgtgg tagccgaccc
      301 cttggtggtg cacgaaggcg agccggggcg ccgcggccag gaggtcctcg ctgaaacgcg
      361 tggcggcgac gatgaccgca tcggcctcgg ccaacttctg cttgcgctcg ccatcatcgt
      421 cggcctccag cgtcatcagc tcgacgtcct cgccggccgc cgccctgatc atggcgtaga
      481 gggcctgatc gccgtggctg ttgtagaaga ctcgcttgcg attgcgcatc accctttcct
      541 cactgcatcg ccataggccg gatcgcccga tcgccatagg ccagatcgcc cgatctgcgc
      601 tagtcttggc ggcgaacccc tccgccggga ctggtgccgg tcagccagca tcaacacaaa
      661 cgcacgtttt tcaagccacg ggagacaacg catgccagac gtgttgggtt ggcgggcaaa
      721 gttcggcgtc ttggccccat cgacgaatac cgtggtcgag cccgacttcg ctgacctgcg
      781 ccctgccggc gtcaccaacc acttgagccg catcttcacg cccaacgtca aggggctcag
      841 caacgagacg ttccggggtg gcgtcgccac catcagcggc aatgtcatgg acgcgctgaa
      901 gagcgtgatg acctgtgagc ccgaccatct ggtgatgggc atgtccggca tcaccttctt
      961 gggcggcatg gcgggcgccg atgccttcgt gcgccaggtc gaggcggagt caggcatcgg
     1021 catttccatc ggcagccacg ccgcggccgc cgcactcagg gcctatggcg gggtgtcccg
     1081 catcgccatc ctgtccccct actggccggt catgaacgag gaggtgcggc gctatttcac
     1141 ggaagcgggt ttcgaggtgg tgcgcgatca tgccatgcaa gtgacgtcgt gggtgggaat
     1201 tgccgcggtc actccggcgc aatgccgcag cgcgctcgag cgcctggacg gcgacgacgt
     1261 cgatgccatc gttcaggtcg gcaccaacct gtccatgctg cgcctggccg ccgccgccga
     1321 gctgtggctc ggcaagcccg tgctcgccat caatgccgca acctactggc atgcgctgcg
     1381 cagcaatggt atgcgcgagc cgatcgccgg cttcggccgg ctgttcgagg ctttttagcc
     1441 gggcgccgag gcgcgccggc aagagccggc gccgacaatc agcctgaggg accaggaaaa
     1501 gcgatggccg agcaaggaaa tcccgcgcct ggcgcgcccg cgccagattt cgacgtggcg
     1561 atcgtcgggg cgggctttgc cggcatgtac atgctgcacc gcctgcgcag cctgggcttc
     1621 cgggttcgcg tcttcgaagc tggtggcgac gtcggtggca cctggtattg gaaccgctat
     1681 cccggcgcgc gctgcgacgt ggagagcatc caatactcct atcaattcga cccggcgctc
     1741 gagcaggagt gggactggag cgaacgctat gcggcccagc ctgagctgtt gcgctatgcc
     1801 cggcacgtgg ccaagcgctt cgatctcaac cgcgatattg cgttcaatac ccgcatcacc
     1861 caggcaagct tcgaggcaac ggccggcgtt tgggtgctcg caagcgatcg aggccgcttt
     1921 accgcgcgct tttgcgtgat ggcaacgggc tgcctttcct cgcccaacct gcccgccttg
     1981 cccgggctcg agagcttcca aggcgagcgc taccacacgg ggcagtggcc gcacgaggag
     2041 gttgatttta ccggcaagcg cgtggcggtg atcggcacgg gctcctcggc ggtgcagtcg
     2101 atcccgatca tcgcgcgcca ggccaagcac ctctacgtgt ttcagcgcac gccaaactac
     2161 gccgtgccgg cgcacaatcg gccgctcgct gccgaggagc tgagaaaggc caaggccgac
     2221 tacccggcca tgcggcgcag ggccaagcag accatgaccg gcatcgactt cgactatagc
     2281 gacaagaagg cgcttctgac cccggcgccg gaacgccagg cggagttcga gcggcgctgg
     2341 gtccacggtg gcctctggtt cctcggcgcc taccaggacc tgatggtcga tcagcaggcc
     2401 aacgacaccg ccgccgactt cgtccgcagc aagatccgcg cgagggtggc cgatcccgag
     2461 ctcgcccgcc ggctggcacc gcaaaatacc atcggctgca agcgactatg cgtcgacatc
     2521 ggctactacg aaaccttcaa ccgcgataac gtcaccttgg tggatgtcag cgacgcgccg
     2581 atcgaggcca tcacgccggc cggcctcaaa gccggcggcg
//
LOCUS       DASTMT010000234         3636 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_2419077, whole genome shotgun sequence.
ACCESSION   DASTMT010000234 DASTMT010000000
VERSION     DASTMT010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3636)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3636)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3636
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2419077"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            175..474
                     /locus_tag="VFR00_05520"
     CDS             175..474
                     /locus_tag="VFR00_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3572 family protein"
                     /protein_id="HEU0158746.1"
                     /translation="MLKAAQSKQSVDRETAETIAAQGLAFVAADAHRLQNFLTLTGIT
                     PAELKRRIGTAEVLLAVLNFLAQDESALLTFAASQQIAPESIGAALARLDAPDVA"
     gene            464..1405
                     /locus_tag="VFR00_05525"
     CDS             464..1405
                     /locus_tag="VFR00_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEU0158747.1"
                     /translation="MLPEAGTLSCRIGIDLGGSKIAGIALGRDGAILASRRVVAPQQD
                     YAATVHAIADMVAALEQAAGRPGTVGIGMPGSMAPGGGGVQNANSTWLNGQPFASDLE
                     GSLNKPVRLANDANCFALSEAVDGAGAGARSLFGVILGTGCGGGLIFEGKLIDGPRGI
                     GGEWGHNPLPWAVAAEHPGPACWCGRNGCMETWVSGPGLELDHRRHTGLALSTAAIAA
                     NAAAGEAAARATLERHASRLARGLAHVVNIFDPELVVLGGGLSQLTHLYSQLPALIAP
                     YILARDQRVSIKPPRWGDAGGVRGAAWLWPQSEALER"
     gene            complement(1442..2308)
                     /locus_tag="VFR00_05530"
     CDS             complement(1442..2308)
                     /locus_tag="VFR00_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity EipB family protein"
                     /protein_id="HEU0158748.1"
                     /translation="MMHPSSRHRLAALAVGAALQLAATPAWAGLQRVVLAPHRAIYEM
                     TLAEARGGAGVTAVSGRMVYELTGSACEGYTQNMRFVTQMTNQKGAVTLTDLRSSSWE
                     EGTGKRFRFNSNQFRDEKQTEATAGDAARANGADAAKVELTKPTSKHLALPQRVYFPI
                     QHSIALLDAANTGKRFFRADLYDGSEKGEKVYDTISVIGSVVAAGGNRKLARVKNAER
                     LDGIAAWPVSIGYFEPNSDKTDAVPVYELTFWFFENGVSRKLFIDYGEFAIQGELAQI
                     TFHEPSKCDQPK"
     gene            2504..3280
                     /locus_tag="VFR00_05535"
     CDS             2504..3280
                     /locus_tag="VFR00_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HEU0158749.1"
                     /translation="MGGVALDRSVFLRPIAHRGLHNKSRGLIENTAPAFLAAIEHDYG
                     IECDLQPARDGTPMVFHDATLDRLTRGHGRIAAHTPQELSRVRYKQQDLGILSFLDLL
                     ELVAGRVPLLVEIKSDGRLPKGFLPAIAQQAAGYQGPIALMSFDRAVVSQCARLAPDV
                     ARGIVIGSHQLASNWWAGPSAAGQNAAVAAVLNKAPAEISFFAIDVRMLEPAKAWLAA
                     HAPAATLFTWTVRTAKERATAARWADAPIFEAAAGAGAMS"
     gene            3401..>3636
                     /locus_tag="VFR00_05540"
     CDS             3401..>3636
                     /locus_tag="VFR00_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013418949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidogalycan biosysnthesis protein"
                     /protein_id="HEU0158750.1"
                     /translation="MSRPCDARVEVIPRIADIAPAAWDACANPAAADLPHNPFIAYDF
                     LRSLEDSGSAVARAGWQPQHLVAESAGGDVLGAVP"
BASE COUNT          570 a         1265 c         1206 g          595 t
ORIGIN      
        1 gccggtgatg gccccaatgc tctcctgata cgtcatgcgg cggcccgctc tcccgcttcc
       61 gcgtactccc tgcacgattc tacgcggctc tggcagccga ctttgcttca gaacacctaa
      121 ccaactgttt aagatgtgcc cgacgccgac ccgccaagaa ggggaaaagg caatatgctt
      181 aaagccgctc aaagcaaaca atcggtcgat cgcgaaacgg cggaaacgat tgccgcgcaa
      241 gggcttgcct tcgtggcggc agacgctcac cggctgcaga acttcctgac gctgacgggc
      301 atcacgcccg ccgaactcaa gcgccgcatc ggcacggccg aggttctcct cgccgtcctg
      361 aacttcctcg cccaggacga atcagccctg ttgaccttcg ccgccagcca gcaaattgcc
      421 cccgaaagca tcggcgcggc gctggcccgc ctggacgctc ccgatgttgc ctgaggccgg
      481 caccttgtcc tgtcgcatcg gcatcgatct cggtggcagc aagatcgccg gcatcgccct
      541 cggcagggat ggggcgatcc tggccagccg gcgcgtcgtt gctccgcagc aggactatgc
      601 cgccaccgtg cacgccatcg ccgacatggt ggcggccctc gagcaggccg ccggccggcc
      661 gggcacggtc ggcatcggca tgcccggctc gatggcgccg ggcggcggcg gggtgcagaa
      721 cgcaaactcc acttggctca acggccagcc gttcgccagc gatctggaag gctcgctcaa
      781 caagcccgtg cgcctcgcca acgatgccaa ctgctttgcc ctctcggagg cggtcgatgg
      841 cgccggcgca ggcgcccgct ccctgttcgg ggtgatcctc ggcaccggct gcggcggcgg
      901 gctcatcttc gagggcaagc taatcgatgg accccgcggc atcggcggcg agtggggcca
      961 caatcccttg ccgtgggcgg tggcggcgga gcacccaggc cccgcctgct ggtgtggccg
     1021 caacggctgc atggaaacct gggtctccgg gccgggcctc gaactcgatc accggcgcca
     1081 caccggcctt gccttgtcca ccgccgccat cgccgccaat gccgccgccg gggaggccgc
     1141 ggcgcgggcc acgctcgagc gccacgccag ccgactagcg cgcgggctcg cgcacgtcgt
     1201 caacatcttc gatcccgagc tcgtcgtgct gggcggcggg ctctcgcagc tgacccacct
     1261 ttattcgcaa ttgccagcct tgatcgcgcc ctacatcttg gcgcgtgacc aacgcgtctc
     1321 aatcaagccg ccacgctggg gggacgccgg cggcgtgcgc ggcgcggcct ggctctggcc
     1381 gcagagcgaa gccctcgagc gctgagcgtt cggcgtgatc gcggccggcg cttggcatcg
     1441 cctatttggg ctgatcacac ttgcttggtt cgtgaaaggt gatctgggcg agctcgccct
     1501 ggatggcgaa ctcgccgtag tcgatgaaca gcttgcggct gacgccgttc tcgaagaacc
     1561 agaacgtcag ctcatagacc ggcaccgcgt cggtcttgtc cgaattgggc tcgaagtagc
     1621 caatcgaaac gggccaggcg gcaatgccgt cgaggcgctc ggcattcttg acccgcgcca
     1681 gcttgcggtt gccgccggcg gcaacgacgc tgccgatcac ggagatcgtg tcatagacct
     1741 tctcaccctt ctccgagccg tcgtagagat cggcacggaa aaagcgcttg cccgtgttgg
     1801 ctgcatccag cagggcgatc gagtgctgga tgggaaaata gacgcgctgc ggcagggcca
     1861 ggtgcttgga ggtcggcttg gtcagttcga ccttggcggc atcggcgcca ttggcacgcg
     1921 cggcgtcgcc ggcggtggcc tcggtctgct tctcgtcgcg aaactggttg gaattgaagc
     1981 ggaagcgttt gccggtccct tcctcccaac tggacgagcg caagtccgtc agcgtcaccg
     2041 cgcccttttg attggtcatt tgcgtgacga agcgcatgtt ctgcgtatag ccctcgcagg
     2101 ccgaacccgt cagctcgtag accatgcggc ccgacaccgc cgtcaccccg gccccaccgc
     2161 gggcctcggc cagcgtcatc tcgtagatgg cgcgatgcgg cgccagcacg acgcgctgga
     2221 gaccggccca ggcaggcgtc gcggcgagtt gtagggcggc gccgacggcc aaggcggcga
     2281 gccggtgcct ggaactgggg tgcatcattg tgccttcttt cccgaacaga agggcagtac
     2341 tagggcctgc gcaccccccg gtgggggctg aggccgtctc tgtctagcag agggcgcctc
     2401 ttgtgcacag aagggcgcgc cggaatgatt gaccgaaacg gcacgcgatg gggtgttgat
     2461 ggccaaagtg ccgcctgctc cgaggtcggg ccgccggccg gcaatggggg gcgttgccct
     2521 cgatcgcagc gttttcctgc gcccgatcgc ccaccgtggc ctgcacaata aaagccgcgg
     2581 gctgatcgag aacaccgcgc ccgccttcct cgccgcgatc gagcacgact acggcatcga
     2641 gtgcgacctg cagcccgcgc gcgatggcac gccgatggtg ttccacgatg ccacgctcga
     2701 ccgcctcacg cgcgggcacg gacgcatcgc cgcgcacacg ccgcaagagc tgagccgggt
     2761 ccgctacaag cagcaagacc tcggcattct gagcttctta gacctgctcg agctggtggc
     2821 gggtcgggtg ccgttgctgg tggagatcaa gagcgacggc cgcctgccga aaggcttctt
     2881 gcccgccatc gcccaacagg cggcaggcta tcaaggaccc atcgcgctca tgtcctttga
     2941 tcgcgccgtc gtcagccagt gcgcgcggct ggcgccggac gtcgcgcgcg gcatcgtcat
     3001 cggcagccat cagctggcca gcaactggtg ggcaggaccg agcgccgcgg gacagaatgc
     3061 cgccgtcgcc gcggtcctca acaaagcccc cgccgagatc agtttttttg ccatcgacgt
     3121 gcgcatgctg gagccggcca aagcctggct cgccgcgcac gccccggcgg ccacactctt
     3181 cacctggacc gtgcgcaccg ccaaggagcg cgccacggcg gcccgctggg ccgatgcgcc
     3241 cattttcgag gccgctgccg gcgcaggagc catgtcgtga gggacgggct cggccctgga
     3301 acgggcgttg tgtcataagc gcgctcgtgg aatcctctcc ccgcgcgcgc tattggcttg
     3361 cgcgcccagg acgacttcgc ctaagcgctt gtcgatggcc atgagcagac cgtgtgacgc
     3421 ccgggtcgag gtgatacccc ggatcgccga tatcgcgccc gcggcgtggg atgcctgcgc
     3481 caatccggcg gcggcggacc tgccgcacaa tccgttcatc gcctacgact ttctgcggtc
     3541 gctcgaggat tcgggctctg ccgtggcgcg cgccggctgg cagccgcagc acctggtcgc
     3601 ggagagcgcc ggcggcgacg tcctcggcgc ggtgcc
//
LOCUS       DASTMT010000235         1766 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_782697, whole genome shotgun sequence.
ACCESSION   DASTMT010000235 DASTMT010000000
VERSION     DASTMT010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 1766)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1766)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1766
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_782697"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(272..>1766)
                     /locus_tag="VFR00_05545"
     CDS             complement(272..>1766)
                     /locus_tag="VFR00_05545"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEU0158751.1"
                     /translation="ERMRAKLPKSLLGLLAIPGLRPAAILKLHQLLGVHSPDALAAAC
                     REGKVAKTKGLGSALERKILQGLKIASEGQGRLRMSEAQAILEQTMAELTRLRPALGR
                     ITVAGDLRRACELVSDLRLVAIDPNAEQATEERFGTVRLHTCPPDRFGAVLLRATGSE
                     AHFAQLQALASKQGLTLTANALRRGAQALSVRREDDLYKALGLACIPAELREGTDEIA
                     RASKNALPQLVAMQDLRGVLHLHTDFSDGANTLEEMAEAARERGYAYLGVADHSQSAH
                     YAGGLKPDRIETQHRMVDAFNRRYRGRFRILKGIESDILADGALDYPPEVLERFDFVV
                     ASVHSRFRLARDAQTRRIIAAVSNPFTTILGHLTGRQLLRRPGYDVDIKAVLKACADH
                     GVAVEINGSTWRLELDWRWHRTALALGCMLSINPDAHSIAELDLVRWATAVARKGGVA
                     KENVLNAMSREQILRHLRNRAAARAGRARTGRAPPRRRSKSLALKKG"
BASE COUNT          281 a          533 c          643 g          309 t
ORIGIN      
        1 gcgcattttg gcgaacccgg gccgcaatgg cgcgggctcg ggaggtgcgc aaagccgccc
       61 ccggtcgcga cgccttcgag acattcggat ggcgcaggca aggggacatc gagggccgca
      121 aagacctgag gaccgaggcg cgggcccttc cgcgcagaag gcgcaaattc gcgccgcggg
      181 catcggcgaa gtttccattg gcagcaaggg tttgtccggc cttgagaacg cgggcttcaa
      241 ggtggcccgc aggggccccg aagcgcgctg gtcaaccttt ttttaaagcc agagatttgc
      301 tgcgccggcg gggcggtgcg cgccctgtgc gcgcgcgccc ggcgcgtgct gccgcgcggt
      361 tgcggagatg gcggaggatc tgttcacggc tcatggcgtt gagcacgttt tctttggcaa
      421 cgccgccctt gcgggcgacg gcggttgccc atctgacgag atcaagctcg gcgatggagt
      481 gcgcgtccgg gttgatgctg agcatgcagc ctagagccaa cgccgtgcgg tgccagcgcc
      541 agtcaagctc gaggcgccac gtgctgccgt tgatttcgac tgcaacgccg tggtcggcgc
      601 aggccttgag gacggctttg atatcgacgt cgtagccggg gcggcgcaga agctggcggc
      661 cggtgagatg gccgaggatt gtcgtgaagg ggttcgagac cgcggcgata atgcgcctgg
      721 tttgggcatc gcgcgcgagg cgaaagcggc tgtgcacgct ggcgacgaca aaatcgaagc
      781 gctccagcac ctccggcgga taatcgaggg cgccgtcggc gagaatgtcg gactcgatgc
      841 ccttgaggat gcggaagcgc ccgcgatagc ggcggttgaa ggcatcaacc attcgatgct
      901 gggtctcgat cctatcgggc ttgaggccgc cggcgtaatg ggcagactgt gagtggtcgg
      961 cgacgccgag ataggcatac ccgcgctcgc gcgcggcctc ggccatttct tccagcgtat
     1021 tggcgccgtc cgagaagtcg gtgtgcaggt gaagcacgcc gcgcaggtct tgcatcgcga
     1081 ccagctgcgg cagggcattt ttgctggcgc gcgcgatctc gtcagtgccc tcgcgcagct
     1141 cggctgggat gcaggcaagc ccgagggctt tgtaaaggtc gtcttcgcgt cgcacgctca
     1201 gcgcctgcgc gccgcgccgc agggcgttcg ctgtcagcgt caggccctgc ttgctggcaa
     1261 gcgcttgcag ctgcgcaaaa tgggcctcgc tgccggttgc gcgcaagagc acagcgccga
     1321 agcgatcggg cgggcaggta tggagcctga cggtgccgaa acgttcttcg gtcgcttgct
     1381 cggcgttggg atcgatggcg accagccgca ggtccgacac cagttcgcaa gcccggcgaa
     1441 gatcgccggc aacagtgatg cgtcccagag cgggccgcag gcgcgtcagc tcggccatgg
     1501 tctgctcgag tatggcctgg gcctcgctca tgcgaaggcg cccttgcccc tcgctggcga
     1561 ttttgaggcc ttgcagaatc ttgcgctcga gcgcgctgcc tagcccctta gttttcgcaa
     1621 cctttccctc ccggcaggct gccgcgagtg catcgggtga atggacgccg aggagttgat
     1681 gcagcttgag aatggcggcc ggccgaaggc cggggatggc gagcaggccg agaaggcttt
     1741 tgggaagctt cgcgcgcatg cgctcc
//
LOCUS       DASTMT010000236         2065 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_2749563, whole genome shotgun sequence.
ACCESSION   DASTMT010000236 DASTMT010000000
VERSION     DASTMT010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2065
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2749563"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..399
                     /locus_tag="VFR00_05550"
     CDS             <1..399
                     /locus_tag="VFR00_05550"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEU0158752.1"
                     /translation="SGLRGKPVVVYFYPKDDTSGCTIEAKDFSRLAAEFRKVGVEVVG
                     ISPDSVESHEKFRSKHELTVRLAADADKATAMAYGVWAQKSMYGRTYMGIDRSTFLID
                     KAGRLARSWRKVKVPGHAEEVLAAARGLAT"
     gene            481..738
                     /locus_tag="VFR00_05555"
     CDS             481..738
                     /locus_tag="VFR00_05555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0158753.1"
                     /translation="MLIARSVVAAALLLLPLSAALACDDFAEEQAMAEAVAAAKASRL
                     AEAQQPAAVAAEAQAAPEAREARPTPAAELTATAALVPSRR"
     gene            complement(932..1720)
                     /locus_tag="VFR00_05560"
     CDS             complement(932..1720)
                     /locus_tag="VFR00_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006019869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HEU0158754.1"
                     /translation="MSQVAAARFERIKALPAYELVAEAIERQILGGVLRPGDPIGTES
                     ELVKQFGVNRSTVREGIRLLEQAGLVARESSRRLLVAVPHYDRLATRMSRALILQQVT
                     FRELWYTSMALEPAVVAQAIEHATAEELDAIDANIAATRAVQHSAGAVANLDAEFHGL
                     VAKAAHNRVLLLAKEPSSMLVLPTVELILTNNPAGIARLIEAHQNIAAAIRQRDQAKA
                     LLWVDRHLQDWRAGFERAGCQFDSAVAPINARRLKWDAALPGQQ"
     gene            complement(1776..>2065)
                     /locus_tag="VFR00_05565"
     CDS             complement(1776..>2065)
                     /locus_tag="VFR00_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006022772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HEU0158755.1"
                     /translation="YPTGNLADGSNVFGYSVATLMTIVLKQCGDDLTRANVMKQAANL
                     KNVDLPLLLPGIKVNTSATDFYPLQSVQLQKFEGETWKLFGDVLSNETASN"
BASE COUNT          340 a          679 c          688 g          358 t
ORIGIN      
        1 tccgggctgc ggggcaagcc ggtggtcgtc tacttttatc ccaaggatga tacttccgga
       61 tgcacgatcg aggccaagga cttctcccgg ctggcggcgg agtttcgcaa agtcggcgtg
      121 gaagtcgtcg gcatatcgcc cgacagcgtg gaaagtcatg agaagtttcg cagcaaacac
      181 gagctgaccg tccgcctcgc cgccgacgcc gacaaggcga cggcgatggc ctatggcgtt
      241 tgggcgcaga agtccatgta cggtcgcacc tacatgggga ttgatcgctc aacctttttg
      301 atcgacaagg ccggccgtct cgccagaagc tggcgcaaag tgaaagtgcc agggcacgcc
      361 gaagaggtgc tcgccgcggc acgcggcctc gccacctagc cgcccatcac cgaaccgtga
      421 ctgccattgc gcgccaggct gcgccaaact gaacgagcct tgccagccag ggagacgctt
      481 atgctgatcg ccaggtccgt tgtcgcggcg gcgctgttgc tgctgccgct gtctgccgcg
      541 cttgcctgcg atgattttgc cgaagaacag gcaatggcgg aggccgtcgc ggcggcaaag
      601 gcctcgcgcc tcgccgaggc gcagcagccg gccgccgtgg ccgccgaggc gcaggcggct
      661 cccgaggcgc gagaggcccg gccaactccg gctgccgagc tgacggcaac ggcggccctt
      721 gtgccgagcc ggcgctaatc ctccaccgct aatcccaagg gcgcgaggta gccggcccgg
      781 gatccccatc ccgcgtgcgc gtcaggcacc gccgcaaggc cggctcggca gggcccgctc
      841 gtcgcatggc ttggggcatg gcttgaggca tggctcgggg cgcgctgtcc gcatcatcct
      901 cgacatcgtt ggcaaaggag cctgcgcggc gctactgctg gcccggcagc gcggcatccc
      961 acttcagccg acgcgcgttg atgggcgcca ccgcgctatc gaactggcag ccggcgcgtt
     1021 cgaatcccgc ccgccagtcc tgcaaatgcc ggtccaccca gagcagcgcc ttggcctggt
     1081 cgcgttgtcg gatcgccgct gcgatgttct ggtgtgcctc gatgaggcgg gcgatccccg
     1141 ccgggttgtt ggtcagaatc aactccacgg tcggcagcac cagcatgctg gagggttctt
     1201 tggcgagcag cagcacgcga ttgtgcgcgg cctttgccac cagcccgtga aattcggcat
     1261 cgagattggc gacggcgccg gcgctgtgct gcaccgcgcg cgtggcggcg atgttggcgt
     1321 cgatggcgtc gagctcctcc gccgtggcgt gctcgatggc ttgcgccaca accgccggct
     1381 ccaaggccat cgaggtatac catagctcgc ggaaggtgac ctgctggagg atcagcgcgc
     1441 ggctcatgcg cgtggccagc cggtcatagt gaggcacggc gaccaacagc cggcggctcg
     1501 actcgcgcgc caccaggccc gcttgctcga gcagccgaat accttcgcgc acggttgagc
     1561 gattgacgcc gaactgcttg accaattccg attcggtgcc gatcgggtcg cccgggcgca
     1621 agacgccgcc gaggatctgg cgctcgatcg cctcggcaac cagctcgtag gccggcaggg
     1681 ccttgatacg ctcgaatctg gcggcggcga cctgggacat tgcctacaac ggtggtttgc
     1741 ggccgcccct gatgggcggc cgcctgcttg gaatgctagt tgctggccgt ctcattcgac
     1801 aaaacgtcgc cgaacagctt ccaggtctct ccctcgaact tctgcaactg cacggactgc
     1861 agcggataga agtcggttgc gctggtattg accttgatgc ccggcagcag caagggcaga
     1921 tcgacgttct tcaggttcgc cgcctgcttc atgacgttgg cgcgcgtgag gtcatcaccg
     1981 cactgcttta ggacgatggt catcaacgtg gcgaccgaat agccgaacac attgctgccg
     2041 tcggccaaat tgccggtcgg gtagt
//
LOCUS       DASTMT010000237         1991 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_1113760, whole genome shotgun sequence.
ACCESSION   DASTMT010000237 DASTMT010000000
VERSION     DASTMT010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 1991)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1991)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1991
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1113760"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..361)
                     /locus_tag="VFR00_05570"
     CDS             complement(<1..361)
                     /locus_tag="VFR00_05570"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU0158756.1"
                     /translation="MKVKRIEHVAIAVKSLAKARAIFEDKLGLPFEYEEYLPQYRTKL
                     AMFPVGSSYIELLESEGADTETTKWISQHGEGLFHICLEVEDIEAALAELKAKGVKLL
                     DEAPRLGHANSRIAFLDP"
     gene            complement(485..>1991)
                     /locus_tag="VFR00_05575"
     CDS             complement(485..>1991)
                     /locus_tag="VFR00_05575"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEU0158757.1"
                     /translation="RARKARGTKLIVVDVYRTPTVERADIALIVKPGSDGALALAVMN
                     VLLTEGLLDRDYLGRYTDFDAEVERHIRTRTPQWAAAITGLSVEEIVAFARLYGATRK
                     SFIRVGFGFTRTRNGASAMHAVSCLPALTGAWSRRGGGAFFLSFDKQQWGLDTSLINA
                     SDAIDPSVRILDQSRIGAVLCGEPEALGAGPPVQAMLMQNANSATVAPSTAKVLKGLS
                     REDLFVVVQEQFLTATARHADVVLPATMFVEHDDIYYGLGHTHLTFGPKLVEPRGEAR
                     PNSYTVRELSRLLGAKHASLALSDRELLDHALSGAGVATFAAAAKLGWIDRALPFEAA
                     HFVDGFPQPDGKFHFKPDWRRVGPGHGVMPRLADFSSRYERADARHPYKLVCPPARNF
                     LNSTFSETPTSLSKEGGPCLRLHPRAAERAGVSEGASVRIGNARGSVVLRVRLDAGQQ
                     EMTLIVEGIWPAQAFREQRAINTLIGDDPVPPNGGAGFHDTAVWLEAATNA"
BASE COUNT          334 a          647 c          675 g          335 t
ORIGIN      
        1 tcggatcgag gaaggcaatg cgcgaattgg cgtgcccgag acgcggcgcc tcatcgagaa
       61 gctttacccc cttggccttg agttcggcca aggccgcttc gatgtcctcg acctccaggc
      121 agatgtgaaa gaggccctcg ccgtgctgcg agatccactt tgtggtttcc gtgtcggcac
      181 cttccgattc cagcagttcg atgtagctgg agccgaccgg gaacatggcg agcttggtgc
      241 ggtactgcgg tagatattcc tcgtactcga agggcaagcc gagcttgtcc tcgaaaatgg
      301 cacgcgcctt ggccaggctc tttaccgcga ttgccacatg ttcgatacgc ttgaccttca
      361 tggcagtgct cctggcgcct tggttgcaaa gaagcggttg gtacttgccg gcggcggcat
      421 cgccgcgcgc gctgccttag gcaggcttgc ccgcttggcc aggccatggc tcgggcggcc
      481 gggctcacgc gttggttgca gcttcaagcc acaccgccgt gtcatggaaa ccggcgccgc
      541 cgttgggcgg tacgggatcg tccccgatca gggtgttgat ggcgcgctgt tcgcgaaagg
      601 cctgcgccgg ccagatgccc tccacgatca aggtcatctc ctgctggccc gcatcgaggc
      661 gcacgcgcag caccaccgag ccgcgggcgt tgccaatccg caccgaggcg ccctcgctca
      721 cgcccgcgcg ctcggccgcg cgcggatgca ggcgcaggca cgggccccct tccttgctga
      781 gcgaggtcgg ggtctcggag aacgtgctgt tgaggaaatt gcgggcgggc ggacaaacga
      841 gcttataggg gtggcgtgcg tcggcgcgtt cgtagcggct cgagaagtcg gccaggcgcg
      901 gcatcacgcc atgcccgggc ccgacccggc gccagtccgg cttgaagtgg aacttgccgt
      961 cgggctgtgg gaagccatcg acgaagtggg cggcctcgaa cggcaaggca cgatcgatcc
     1021 agcccagctt ggccgccgcc gcgaaagtcg ccacgcccgc gcccgacagc gcatgatcga
     1081 ggagctcgcg atccgacaag gcgaggctgg catgcttggc gccgagcaag cgcgatagct
     1141 cgcgcacggt gtaactgttc ggccgcgcct ccccgcgcgg ctcgaccagc ttcggcccga
     1201 aggtgaggtg ggtgtggccg agaccataat agatgtcgtc gtgttcgacg aacatggtcg
     1261 cgggcaacac cacgtcggca tgacgcgccg ttgccgtcaa gaattgctcc tgcaccacca
     1321 cgaacaggtc ctcgcggctc agccccttga ggaccttggc cgtacttggc gccactgttg
     1381 ccgagttggc attctgcatc agcatggctt ggaccggcgg cccggcgccg agcgcctcgg
     1441 gctcgccgca cagcaccgca ccaatccggg attggtcgag gatcctgacc gagggatcga
     1501 tggcgtcgct ggcattgatg agcgaagtgt cgagccccca ctgctgcttg tcgaagctga
     1561 ggaagaaggc gccgccaccc cgccgcgacc aggcgccggt cagggccggc aggcacgaca
     1621 cggcatgcat ggccgaggca ccgttgcggg tgcgcgtaaa gccaaatccg acccggataa
     1681 agctcttacg cgttgcgcca tataagcgcg cgaaggcgac gatctcctcg acgctgagcc
     1741 ccgtgatcgc ggcggcccat tgcggcgtgc gcgtgcgaat gtgacgttcg acctcggcgt
     1801 cgaaatccgt gtagcggccg aggtagtcgc gatcgagcag cccctccgtg aggagcacgt
     1861 tcatgacggc cagggccagg gcgccatcgg agcccggctt gacgatcaag gcgatgtcgg
     1921 cccgctcgac agtcggcgta cgatagacat cgacgacgat cagcttggtg ccccgcgcct
     1981 tacgcgcgcg g
//
LOCUS       DASTMT010000238         5384 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_457712, whole genome shotgun sequence.
ACCESSION   DASTMT010000238 DASTMT010000000
VERSION     DASTMT010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 5384)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5384)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5384
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_457712"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            413..1114
                     /locus_tag="VFR00_05580"
     CDS             413..1114
                     /locus_tag="VFR00_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012974252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEU0158758.1"
                     /translation="MAAKPNLLLIPGLLCSPALWAEQVRGLNDIAEIGVADHTRHASL
                     PEIAEAILAEAPPRFALAGLSMGGYIAYEILRQAADRVTRLALLDTGARADAPERRPQ
                     RLGLIALAEQEGAVRAQQELLPLLIHTDRLRDKPLVDTVLQMAADTGVAAFKRQQTAI
                     MARPDNRPLLPTIGCPTLVLVGREDALTPPSLAEEIAAGIPGARLEIIPECGHLSSLE
                     QPEAVNRALRRWLGT"
     gene            1697..1915
                     /locus_tag="VFR00_05585"
     CDS             1697..1915
                     /locus_tag="VFR00_05585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0158759.1"
                     /translation="MTTKFSLAALALVAALLSLPSGASAGFVGRDRFWGDVDGRMHRL
                     WSFSWLCGDRAAAAKPVKVRRHRHVPAK"
     gene            complement(2256..2618)
                     /locus_tag="VFR00_05590"
     CDS             complement(2256..2618)
                     /locus_tag="VFR00_05590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011652774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0158760.1"
                     /translation="MMSEEASRAAATDRIDATFRNGSVTAVGIILGFSLGFLSQWAAN
                     PIAWSRVDIAAAVPIIVGIMLQGKAFADLLSTACLFTRNYERARRTFLLGLTLVAAGV
                     AVALLLDVLGLGPRTLSP"
     gene            complement(2828..4954)
                     /gene="parE"
                     /locus_tag="VFR00_05595"
     CDS             complement(2828..4954)
                     /gene="parE"
                     /locus_tag="VFR00_05595"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012093041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HEU0158761.1"
                     /translation="MPAFRLGALRDLFGKSDNKRVKQAESPPPSQGSLELVASRPIAV
                     PPPETQEVEAKPVARAVRKAAEAANSYTAADIEVLEGLEPVRRRPGMYIGGTDEKALH
                     HLFAEVIDNAMDEAVAGHATWIEVRLEADGFLSVTDNGRGIPVDPHPKFKSKSALEVI
                     MTTLHSGGKFDSKVYNTSGGLHGVGVSVVNALSERLEVEVARGQQLYAMSFSRGMPQG
                     KLKNLGAIKNRRGTKVRFFPDEKIFGKGVAFKPQRLFRMARSKAYLFGGVEIRWSCAS
                     QLIKDDTPAEAVFHFPGGLKEYLEATIEGQTRVTKEVFAGRIDKEGGHGSVEWAACWV
                     ADEDGFLNSYCNTIPTPEGGTHENGLRSALGKALRDFGERVGNRKAAQITADDVLGTA
                     AAMLSVFIREPEFQGQTKDKLATQEATRIVENAVRDAFDHWLADSPQQATRLLEWSID
                     QAEDRLRRRREKEIGRQSATRKLRLPGKLADCSLNSKQGTEIFIVEGDSAGGSAKQAR
                     DRERQAILPLRGKILNVANASSQKLSQNQLLSDLVQALGVGTGSKYRDEDLRYERVIV
                     MTDADVDGAHIASLLITFFYQEMPELIEQGHLYLALPPLYKLSHGPKSVYARDERHKE
                     ELIKREFAANAKVEISRFKGLGEMNAAQLKETTMDPAKRTLLRVEIADDKEVVSDAVN
                     ALMGSKPEARFRFIQERAAFARDLDI"
     gene            5173..>5384
                     /locus_tag="VFR00_05600"
     CDS             5173..>5384
                     /locus_tag="VFR00_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002714118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEU0158762.1"
                     /translation="MTALEKAAPARAAASAERAIWRAEIIDTVRLAAPMALTQLGQVS
                     MMTTDLMLLGRLGDKVVAAAALAHTVL"
BASE COUNT          903 a         1782 c         1769 g          930 t
ORIGIN      
        1 tagccgcaaa gggcctaacg ggaaagcatc tgcttgggga aaatgatcaa taaagcaatc
       61 ccctgcgccc gttacaggct cgtagatggc actgttcgct gcaaaaatca agctgatcgg
      121 gatcacgctt gtgtgggtca gatcgcctcg cacggcgcag tttgcgcgcc ttggagccgc
      181 ccaagtcccg ggggaaatgg ggcgcgtgac gctgccgcgg caaagttgcc gcttggggcg
      241 tgttggggag gttacgcagc gatccggcgc ggcggcgcga tccgctggcg tgccctgccg
      301 gcaacggctg gcggcgcgac gccgctgccg aaggcgtggt cgccacctag tgggtggcgc
      361 aaaacgatgc cattgatcgg gggttttgca gtgcttgccc ccggagagga ccatggccgc
      421 caaaccgaac cttctgctga tcccgggcct gctgtgctcg ccggcgctgt gggccgagca
      481 agtccgtgga ctgaatgata tcgccgagat cggcgtcgcc gatcacacgc gccatgcaag
      541 cctgccggag atcgccgagg ccatcctggc tgaggcgcca ccgcgcttcg ccttggccgg
      601 cctgtcgatg ggcgggtaca tcgcctacga gatcctgcgc caggccgccg atcgcgtcac
      661 gcgcttggcg ctgctcgaca cgggcgcgcg cgccgatgcg ccggaacgcc gtccgcagcg
      721 tcttgggctg atcgcgctgg ccgagcaaga aggagcggtc agggcgcagc aggaattgct
      781 gccgctgctc atccacaccg accgcctgcg cgataagccg ctggtggata cggtgctgca
      841 aatggcggct gacacgggcg tcgccgcttt caagcgacag cagacggcca tcatggcacg
      901 accggacaac cggccccttt tgcccaccat tggctgtccg accctcgtcc tcgttggacg
      961 cgaggacgcc ctgaccccgc ccagcttggc cgaggagatt gccgcgggca tcccgggtgc
     1021 ccggctcgag atcatccccg agtgcggcca tctctccagc ttggagcagc ccgaggcggt
     1081 caatcgggcg ctgcggcgct ggctcggcac ctaagacgcg gggcgggcgc gccgctggct
     1141 ggccggcagg gccgtgcatg gtgccgcggc gctgtgcctg cggtcccggc cggccgttgc
     1201 tgccgaccac cgctggcctc gagaggatcg tcccgcgggc ttgcaaggac catcggaagg
     1261 gaccccagag gggccgcgcc gagctccccg ccacgctcgc gatgcgtcca ccggcccgcg
     1321 agcccgccgc cgaacgagca ggcggctgcc gcggcctcca ggaccaacga caacccgctg
     1381 cgacgcggcc cgccaaagtc acgagggatg tgcgaccggc cggccgtcca acgagctagc
     1441 ccaggggtgg ccagcttgag gcggcgtcgc actggcgaac ccggcgaggc ggtctgcggc
     1501 atcccccggg gaatggcata tacaagtatt aataatgtat gtcgcagaaa ttaatcatct
     1561 gcactcatgt atttagtaac gcgaatactt tgtgctttaa aaacattgga atatcggagc
     1621 agcgacctcg tccaatcgaa ccgcttcttc gctgttttta ggcatccagg cttctcgtct
     1681 ccaaggaggg acatcgatga caaccaagtt cagcctcgcc gcgctcgccc tcgtggcagc
     1741 gcttctcagc ctcccgtccg gcgccagcgc gggcttcgtc ggccgcgacc gcttctgggg
     1801 cgacgtcgac gggcgcatgc accgtctgtg gtctttcagc tggctgtgcg gtgaccgcgc
     1861 ggccgctgcc aagccggtca aagtgcgccg ccaccgccac gtacccgcga agtaagcgaa
     1921 agacctcgcg agtttgcgac cgcaagggcg cttcaccgca agggtgtggc gcccttcgct
     1981 cgttcagcac acgtttgcgt gcgtggcggt tgaaacggcc gcaggggctc cgatgttgca
     2041 aagggaaagc gtcacatcga gtttgggact tcgcccatgg gtgctggtaa gccacatgcc
     2101 gccgacggca aagaaaatgc cgaggcatcg caacacctcg acgaaaggct cgacgccgcc
     2161 ctgatcgaaa cttttcccgc gagcgatccc atcgccgtgg gccggcccac cgccaccgag
     2221 cccgccgcac agccgagccc tcggcgccgc acggctcaag gcgacaaggt gcgcggcccg
     2281 agcccgagaa cgtcgagcag cagcgccacc gccaccccgg cggcgacgag ggtcaagccg
     2341 aggagaaagg tcctgcgcgc gcgctcgtaa ttgcgcgtga agagacaggc ggtggaaagc
     2401 aggtcggcga aagctttgcc ctgcagcatg atcccgacaa tgatgggaac ggcggcggcg
     2461 atatcgaccc tgctccaggc gatggggttg gctgcccact gactaagaaa gcccagcgaa
     2521 aagcccaaga taatgccaac ggcggtgacg gagccgttgc ggaaggtggc gtcgatccgg
     2581 tcagtggcgg ccgcgcggct cgcttcttcg ctcatcatcg cacgctccgc cctacccggg
     2641 actgcctcgc tccggcaacg gccacacctg tcgccggcgc gcgcgcctcg ctgttcggtg
     2701 gccgtgacga tgctcgcagg acgccagcga tccgtcgacc cgggttctcc ccgcctttgc
     2761 cgcgtgcgaa accgtggcga agcatcgcgc cgggggctaa acggcgaagc gcgcgatgca
     2821 cgccggccta gatgtcgaga tcgcgagcga aggcggcgcg ctcttgaatg aaacgaaagc
     2881 gcgcctcggg tttggagccc atcaaggcat ttaccgcgtc gctgaccacc tccttgtcgt
     2941 cggcgatttc gacccgaagc agcgtccgct tggcgggatc catcgtcgtc tccttgagct
     3001 gggccgcgtt catctcgcct agccccttga aacggctgat ctcgactttg gcgttggcgg
     3061 caaactcccg cttgatcaat tcctccttgt gccgctcgtc gcgggcatag accgacttgg
     3121 gcccgtggct cagcttgtag agtggcggca atgccagata gaggtggccc tgctcgatca
     3181 gctcgggcat ctcctgatag aaaaaggtga tcaacaaaga ggcgatgtga gcgccatcca
     3241 catcggcgtc cgtcatgacg atcacgcgct catagcgcag atcctcgtcg cgatacttgc
     3301 tgccggtacc gacgccgagg gcctgcacca gatcggacag cagttgattc tgtgagagct
     3361 tttgcgagga cgcattggcg acgttgagga tcttgccgcg cagcggcagg atggcctgcc
     3421 gttcgcggtc gcgcgcttgt ttggccgaac cgcccgcgga gtccccctcg acgatgaaga
     3481 tctcggtgcc ctgtttggag ttgaggctgc agtcggcaag cttgccgggg agccgcagct
     3541 tgcgcgttgc gctctggcga ccgatttcct tctcgcgccg ccgccgcagg cgatcctcgg
     3601 cctggtcgat gctccactcg agcagtcgcg tcgcctgctg cggggaatcg gccagccaat
     3661 gatcgaaggc atcgcgcacg gcattctcga caatgcgcgt ggcttcctgg gtcgcgagct
     3721 tgtccttggt ttgcccttga aactccggct cgcggatgaa tacggaaagc atggcagcgg
     3781 ccgtgcccag cacgtcatcg gcggtgatct gcgccgcctt gcggttgccg acgcgctcac
     3841 cgaaatcgcg caaggctttg cccaacgccg agcgcaagcc gttctcgtgc gtgccgccct
     3901 ccggtgtcgg gatggtgttg caataggagt tcaggaagcc gtcttcgtcg gcgacccagc
     3961 aggccgccca ctcgaccgaa ccatgcccgc cctccttgtc gatgcgaccg gcaaacacct
     4021 ccttggtcac ccgcgtctgg ccctcgatgg tcgcttccag atattccttg agaccaccgg
     4081 gaaaatggaa caccgcctcg gccggcgtat cgtccttgat gagctgggac gcacatgacc
     4141 agcggatctc gacgccgccg aacaaatagg ccttcgagcg cgccatgcgg aacaggcgct
     4201 gcggcttgaa ggctacgccc ttgccgaaga tcttctcgtc gggaaaaaag cgcactttgg
     4261 tgccacggcg gttcttgatc gcgccgaggt tcttgagctt gccctggggc atgccgcgac
     4321 tgaacgacat ggcgtaaagc tgttggccgc gggcaacctc cacctcgagc cgttcggaaa
     4381 gcgcgttcac caccgagacg ccgacaccat gcaggccgcc gctggtgttg tagaccttgc
     4441 tgtcgaactt gccgcccgag tgcagcgtgg tcatgatgac ctcgagcgcg gacttcgact
     4501 tgaatttcgg gtgcggatcg accgggatgc cgcggccatt gtcggtcacg gacaggaagc
     4561 cgtccgcctc caggcgcacc tcgatccacg tggcgtgccc cgccaccgcc tcatccatgg
     4621 cgttgtcgat cacctcggcg aacaggtgat gcagagcctt ctcgtcggta ccgccgatgt
     4681 acatccccgg gcgccggcgt acgggctcga gcccctcgag cacctcgatg tcggcggcgg
     4741 tgtagctgtt ggcggcttcg gcggctttgc ggacggcacg ggccacgggc tttgcttcta
     4801 cctcttgcgt ttctggcggc ggaacggcga tcggacggct tgcaaccagc tccaggcttc
     4861 cctgtgacgg cggcgggctt tccgcttgct tcacgcgctt gttatcggat ttgccgaata
     4921 ggtcgcgcaa ggccccgaga cgaaaggctg gcatggctgt ttgcgcccgt aatgctggag
     4981 ttggactgct tttgcaatgc cacacacaaa cccgcgaggc ccggcgcgaa tctcctggca
     5041 agcgcagggc ggcgcctcag ttgcatggga ggggcggctt tggcaagccg tcgcccgggc
     5101 ggccgggggg ctggccggca gcgcgccagg cgcatccttt ccttttgcgc aaagcaccct
     5161 atggtgcgcg ccatgacagc actcgagaag gcggcacccg cccgcgccgc ggcgtcggcg
     5221 gagcgggcta tttggcgcgc cgaaataatc gacaccgtcc gcctggcggc gccgatggcg
     5281 ctgacccagc tcggccaggt cagcatgatg accaccgatc tcatgctgct cggccgcctg
     5341 ggcgacaagg tggtggcggc ggcggcgctg gcccacaccg tgct
//
LOCUS       DASTMT010000239         3357 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_785595, whole genome shotgun sequence.
ACCESSION   DASTMT010000239 DASTMT010000000
VERSION     DASTMT010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3357)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3357
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_785595"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..289)
                     /locus_tag="VFR00_05605"
     CDS             complement(<1..289)
                     /locus_tag="VFR00_05605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0158763.1"
                     /translation="MPRTLGRWTLPVALVLAAAPSGRPIFAQVPTAPAAAETEEERRE
                     RETRTACAVAVCATLHKHEPASGQVSCPVTKTWRKEVLEKLLAPAKLSWPWG"
     gene            complement(368..1102)
                     /locus_tag="VFR00_05610"
     CDS             complement(368..1102)
                     /locus_tag="VFR00_05610"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEU0158764.1"
                     /translation="MDWSSPAFVLAGALIGGLLNGLTGFGTGLTALPLWLQVLEPVVA
                     AQLVSVASITGHVSALPALWRDSDWRELGPMLGAGLIGVPIGLCILPFIKVEVFKMSV
                     GILLIGYCTFMLGAAGHLHIRRAGAAAEMLIGFLGGVLGGIAGLSGPPPIVWGTLRAW
                     PKARRRRTLQAFNTAILAAMLLASLLGGLIGMRLLVASAIALPATLLGNWLGERLYRR
                     LDERRFDRVVLGLVFLSGCILVASNG"
     gene            1736..2524
                     /locus_tag="VFR00_05615"
     CDS             1736..2524
                     /locus_tag="VFR00_05615"
                     /inference="COORDINATES: protein motif:HMM:NF018420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1194 domain-containing protein"
                     /protein_id="HEU0158765.1"
                     /translation="MTAYVALALGFAAGALAQLLVDLELVLAVDVSLSMDLDEQRLQR
                     DGYITAFRDGEVQKAITSGPHGRIAVTYMEWAGPSVQNVVVPWTIIDGSAAAHALADK
                     LEAQPISRARMTSISQGLVFAAQLFDSSGVKGIRRVIDVSGDGPNNAGPLVTLVRDQV
                     VAQGIAINGLPILLKKPASFFDIADLDVYYRECVIGGAGAFMIPVRERFEFKTATRRK
                     LLLEIAGGEQPAWLIPAQLREPVSGGDCTFGEQQWRRYLDTGPN"
     gene            2634..3302
                     /locus_tag="VFR00_05620"
     CDS             2634..3302
                     /locus_tag="VFR00_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extensin family protein"
                     /protein_id="HEU0158766.1"
                     /translation="MLLSRVPPCLVPLLALALWACSQTPQFVARDEPWRADDERACLA
                     AGIVRESAFVALRASLGGPAVCGALKPFSVSAAVNGAVELRPPALLRCPMVPAIDHWV
                     ERVVMPAARRHLRGELGVVKIAGSYSCRPMNNVDGALLSEHGHANAIDVAGFVLADGR
                     SVDVKSGWWGVFAQRNFLREVHHGSCALFSTVLGPNYDANHRDHFHLDLARHGRDGGN
                     TICK"
BASE COUNT          588 a         1140 c         1074 g          555 t
ORIGIN      
        1 agccccacgg ccagctcaat tttgccggcg ctaggagctt ttccagtacc tccttgcgcc
       61 aggtcttcgt aaccgggcag gacacctgcc cactggcggg ctcgtgcttg tgcagcgtcg
      121 cgcaaaccgc caccgcgcag gccgttcggg tctcccgctc gcgcctttcc tcctccgtct
      181 ccgccgcggc cggcgcggtg ggcacctggg caaatatggg ccgacccgag ggggcggccg
      241 ccagcacgag ggcaacgggt agggtccagc ggcctagcgt tcgcggcatt gatttttctc
      301 aatcttgctc gagcgcggtt gtggccgagc aacggcccag aaatatgaca gcggggcgct
      361 cgggcgctta gccgttcgag gcaaccagga tgcaaccgga caagaacacc agtcccaaca
      421 cgacgcgatc gaagcggcgc tcatcgagac gccgatagag acgttcaccc agccaattgc
      481 ccaacagggt tgcgggtaac gcaattgccg aagcaacgag caggcgcatg ccgataaggc
      541 caccgagcag gctcgccagc aacatggccg cgagaatggc tgtattgaag gcctgcagcg
      601 tccgtcgccg ccgcgccttg ggccaagctc gcaacgtgcc ccatacgatc ggcggcggac
      661 cggacagccc agcgatgccg ccgagcacgc cgccgaggaa gccaatcagc atttccgcgg
      721 ccgcgccggc acgacgaata tgcagatggc cggccgcacc cagcatgaac gtgcaatagc
      781 cgatgagcag gatgccgacg ctcattttga atacctcgac cttgatgaag ggcaggatgc
      841 acagaccgat cggaaccccg atcaatccgg cacccagcat gggaccgagc tcgcgccagt
      901 cgctgtcgcg ccagagcgcg ggcagggcgc tgacatgccc ggtgatcgag gccaccgaaa
      961 cgagctgggc cgccaccaca ggctccagga cctggagcca cagcggcagc gccgtgagcc
     1021 ccgtcccgaa gcccgtcagg ccgttgagaa ggccgccgat gagggctccc gccagaacaa
     1081 atgcggggct ggaccagtcc atgcggatgt cctgaagttg tacgggtgaa aacctatgcc
     1141 gcgcgggcct ggcggcaggg agccccaccg agctccccgc gtttatcgcc aaagccagcg
     1201 cgggcgtcaa aaccacgggc tgcgatcgtg cgcaaccagc cccgcgcggc ggccactccc
     1261 aggggcacgg gccgcaatcg agggggcaag gaggaagagg ggcgaaggag ccctgccgag
     1321 cgggcgatga gctgaaccac cggccaggga gacggcgccc gcaccgctgc cagtcgcgat
     1381 gacggacacg aaaactgtcc atcacgccag ccggtagccc catgcgagcg ccgtaaggca
     1441 caccgcgcgt tacgcaactc tcacgccacg caactctcac gcacgcggga cgccgaacgc
     1501 acacatggcc tcttcgcgcc cgggcacgct agcagcagct acaactcttg tccgatcgcg
     1561 tcgccgccgc cattttgaaa acctgtggat aagcctgtga gcgtgccgcc aagttgcgct
     1621 cgggtgtccg cccgagaagg tcgcaattgg ccttttccgt gcgctatcgc catgccagtg
     1681 tggtcgcatg cgaggccccg cgtgcacgac cgccctcaag ttgggttttt gcgccttgac
     1741 ggcttatgtc gccctggcgt tgggctttgc cgctggcgca ctggcccagc tcctcgtcga
     1801 tctcgagctg gtgctcgccg tcgacgtgtc cttgtccatg gatctcgacg agcagcgcct
     1861 gcagcgtgac ggctacatta ccgcctttcg cgacggggag gtgcaaaagg cgatcacgtc
     1921 gggaccgcat ggccgcattg ccgtgaccta catggagtgg gctggtccct ccgtgcaaaa
     1981 cgtggtcgtg ccctggacca tcatcgacgg ctcggcggcg gcacatgcct tggccgataa
     2041 actcgaggcg caaccgattt cccgggcgcg catgacctct atttcgcagg gcctcgtgtt
     2101 cgcggcccag ctgttcgaca gcagtggcgt caaggggatc agacgcgtga tcgacgtttc
     2161 gggggacggg cccaacaatg ctgggcccct cgtgactttg gtgcgcgacc aagtggtggc
     2221 gcaaggcatt gccatcaacg gtcttccgat tctgctgaaa aagccggcca gctttttcga
     2281 catcgccgac ctcgatgtct actaccgcga gtgcgtgatc ggcggcgccg gcgcctttat
     2341 gatcccggtg cgcgagcgtt tcgaattcaa gacggccacg cgccgcaaac tcctgctcga
     2401 aattgccggt ggcgagcagc cggcctggct gatccccgcg cagctgcgcg agcccgtcag
     2461 cgggggggat tgcacctttg gcgagcagca atggcgccgg tacctggata cggggcccaa
     2521 ttgagccgca ggccggcgca aagctttacc aacctgcctc aaacgcctcg ccaccaccgc
     2581 cgcgaacggc ttggttaagg ccgcattaag ggggtgcgcc tattgtctgt agcatgctcc
     2641 tgtcgcgcgt tccgccgtgc cttgtgccgc tgctggcgct cgccctttgg gcgtgcagcc
     2701 aaacgccgca gttcgttgcc cgcgacgagc cctggcgcgc ggacgacgaa cgcgcctgcc
     2761 tcgccgccgg catcgtgcgc gaaagtgcct tcgtcgcctt gcgcgcgagc ctcgggggac
     2821 ctgcggtgtg cggcgccctt aagccgttca gcgtctcggc ggccgtcaat ggcgcggttg
     2881 aactacgccc gcctgcgctc ctgcgctgcc cgatggtccc cgccatcgac cactgggtcg
     2941 agcgcgtggt catgccggcg gcgcggcgcc atttgcgtgg cgagctcggt gtcgtgaaaa
     3001 tcgccgggtc ctattcctgc cgcccgatga acaacgtcga cggcgccctg ctatccgagc
     3061 acggccacgc caacgccatc gatgttgccg ggttcgtcct tgccgatggg cgcagcgtcg
     3121 acgtcaaaag cggctggtgg ggcgttttcg ctcagcgcaa tttcctgcgc gaggtccacc
     3181 atggcagttg cgccctgttc agcacggtgc tcggccccaa ttacgatgcc aatcaccgcg
     3241 accattttca tctcgatctg gcccggcacg gccgcgacgg gggcaatacg atttgtaagt
     3301 agcgacgcgg cagtttttct gccgcacccc acaagcgcaa acgacgtttc ccgctcg
//
LOCUS       DASTMT010000240        10760 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_1438913, whole genome shotgun sequence.
ACCESSION   DASTMT010000240 DASTMT010000000
VERSION     DASTMT010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 10760)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10760)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10760
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1438913"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..553
                     /locus_tag="VFR00_05625"
     CDS             <1..553
                     /locus_tag="VFR00_05625"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011580620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEU0158767.1"
                     /translation="MREQLIREVEIIPLEVVVRNVAAGSLAQRLGLEEGKALPRSIIE
                     FYYKNDALGDPMVSEEHVTAFGWATPPEIDEIMALAIRVNDFLVGLFSGIGIRLIDFK
                     VEFGRLWENEQMRVVLADEISPDCCRLWDAATSDKLDKDRFRRDLGGVLEAYQEVARR
                     LGVLTENPRPKGSGPVLVSSKPH"
     gene            636..878
                     /gene="purS"
                     /locus_tag="VFR00_05630"
     CDS             636..878
                     /gene="purS"
                     /locus_tag="VFR00_05630"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HEU0158768.1"
                     /translation="MKARIKITLKGGVLDPQGKAIEGALAGLGFTGVREVRQGKYIEV
                     DLAESDEVRAREQVERICKELLANTVIENYAYELSR"
     gene            875..1534
                     /locus_tag="VFR00_05635"
     CDS             875..1534
                     /locus_tag="VFR00_05635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0158769.1"
                     /translation="MRKPRRLAGGFERMVALARFSLAPLACLLAALLAPLAAQQIPAS
                     MLTVESTAPHRYQVTVPAGCRQEEGPGTLDAICSAEFDAEKSAEASAAASLVFEVGAE
                     AVAGDADKAPSQLAAGYGEDVFKSELPEAVCGEADRARVKIENVKQVLEEARVAYTAD
                     VLCSEVKFLGLSERRASVKVVVTPGVRYRLLARAPQGEYEQHKHLIEAFFASFRVLPT
                     G"
     gene            1544..2233
                     /gene="purQ"
                     /locus_tag="VFR00_05640"
     CDS             1544..2233
                     /gene="purQ"
                     /locus_tag="VFR00_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013418127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HEU0158770.1"
                     /translation="MHAAVIVFPGSNCDRDVKVALEKVTGHPVSLVWHGDAAVPDSDL
                     IVLPGGFAYGDYLRTGAMAAHSPIMRDVVAKAKAGTPVLGICNGFQVLTEAGLLPGVL
                     MRNASLKFVCKDVYLRVEQPDTPFTARYRQGEVIRIPVAHGDGNYFADAATLDALEGE
                     GRVVFRYVDAGGNRSLEANPNGAQRDIAGICDARRRILGMMPHPERLFEPALGGCDGR
                     GVFESLLASLN"
     gene            2476..3723
                     /locus_tag="VFR00_05645"
     CDS             2476..3723
                     /locus_tag="VFR00_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011503404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TCR/Tet family MFS transporter"
                     /protein_id="HEU0158771.1"
                     /translation="MQKPVPGAERRKAAIAFIFAIVLLDVIALGIVIPVLPKLVESMV
                     GETARGAEIFGLFGTAWALMQFLCSPLLGALSDRFGRRPVLLISMAGHGLDYILMALA
                     PNLLWLFVGRVISGITAASFGTAYAYIADVTTPEKRAQSFGVVGAAFGVGFVLGPALG
                     GILGAYDPRLPFWVAAAFSLANAAYGYLFVPESLPEDRRMAFSLARANPVGSLRLLRQ
                     RPELLGLAGVNFLHQLAHASLPAIAVLYTSYRYGWDSQTMGLSLAAIGIMSGLVQGTL
                     IKPIVAHVGERWTLLAGLLFGAAGFAIYGFARTSVEFWAGIPVMALWGIAGAATQAMM
                     SQLVTPSEQGQLQGANASIMAVAGLFGPGLFSFTFAWSIARGEAAGWPGLAFLLAAAL
                     LVLATALAWQVTRTAEVSEPGAN"
     gene            3725..4795
                     /locus_tag="VFR00_05650"
     CDS             3725..4795
                     /locus_tag="VFR00_05650"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEU0158772.1"
                     /translation="MTGAADLCFVISADYARRTGGWIYDHRLLQDLRGRGWRIEEAIL
                     PAGFPQPSPTARARAAAVFRAIPDGRVVLVDQLCLGVLPEVARAEGRRLRLVMIVHHP
                     LALEGYASAEQRARVQRAEKEALGQVAAVIVSSPNTARALMLDYAVSADRIIVAIPGI
                     DRRALARGSGGRVLNLLSVGALVPRKDHALLLRALAGLRHLPWRLQLVGNVTRAPQHV
                     GRLRGIIGACGLAGRVSVRGELPEHRLLRLWLGADLYVSASRHEGYGMALAEAFAHGI
                     PVVSTYAGAAGAWVGRRGARLVRGNSVASLRAALGEVLGSSARRAALQVGARARRPSL
                     PRWQATAGSVDRRLRQLLESRS"
     gene            4877..7087
                     /gene="purL"
                     /locus_tag="VFR00_05655"
     CDS             4877..7087
                     /gene="purL"
                     /locus_tag="VFR00_05655"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008960447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HEU0158773.1"
                     /translation="MHEQSKARFSPEVIAEHGFKGDEYQRLLQILGREPSLTELGIFS
                     VMWSEHCSYKSSRRWLRELPTKGPAVIQGPGENAGVVDLGDGDCAVFKMESHNHPSYI
                     EPYQGAATGVGGIMRDVFTMGARPVANLNALRFGAPAHPKTRHLVAGVVAGIGGYGNC
                     TGVPTVGGETNFDAGYNSNILVNAMCVGLAKTHKIFYSAAKGVGLPVVYVGSKTGRDG
                     IHGATMASAEFDDKSDEKRPTVQVGDPFTEKLLIEACLELMASDAIVAIQDMGAAGLT
                     SSTSEMADKGGVGIELDLDQVPQRETGMSAYEMMLSESQERMLMILNPGREPEAQAIF
                     KKWGLDFAVIGSTTDSGRLIVKHRGVVEADLPVDKLANSAPLYERPYVETAPPRAILP
                     EWVPAPNTILGTLKTMLGSPQLASRRWIWEQYDHMVMGDTVQRPGGDAAVVRVHGTSK
                     GLAMTCDVTPRYVAADPRMGAKQAVAESWRNLIAVGADPIAITDNLNFGNPERPEVMG
                     QLVGAIKGMREACEALKYPVVSGNVSLYNETNGIAIPPTPAIGGVGLIPDIGKMASIA
                     LKTTGDLLLAVGREAGHLGQSLYQLSITGKLEGSPPPVDLADEIKAGNLVRSLIREGK
                     VRAVHDVSDGGLLTAVAEMALAGSLGVQLYPYEGRLPQHAAWFGEDQGRYVLAADPEL
                     AEEIIERSRLLALPARVIGRVGGEALVLKEEAVLPLAELRAAHEDWLPRYMRVP"
     gene            7215..7451
                     /locus_tag="VFR00_05660"
     CDS             7215..7451
                     /locus_tag="VFR00_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family transcriptional regulator"
                     /protein_id="HEU0158774.1"
                     /translation="MPMDSQDIARLIKARFPDAEVTIRDLAGDGDHYAAHVVSKEFRG
                     KTRVQQHKMVYEALEGRMGGVLHALALTTAPRPD"
     gene            7539..7889
                     /gene="grxD"
                     /locus_tag="VFR00_05665"
     CDS             7539..7889
                     /gene="grxD"
                     /locus_tag="VFR00_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013418007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HEU0158775.1"
                     /translation="MSEQAVHDWIRKAVASNDVVLFMKGTKDFPQCGFSSQVAQILGH
                     LGVTYKDVNVLDDMGIREGIKSFSSWPTIPQLYVKGEFVGGCDIVREMFQEGELQQLL
                     DAKGVNYNRAKATV"
     gene            8002..9078
                     /locus_tag="VFR00_05670"
     CDS             8002..9078
                     /locus_tag="VFR00_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013947046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="paraquat-inducible protein A"
                     /protein_id="HEU0158776.1"
                     /translation="MTLGGRRFLLTLAIVGASVCLALGVSLPIIKLTKFVFFTYEHSL
                     ISTVNALIRSGQIFLGITVLIFSIALPILKLLYLLLLSTLPKRELQRLSRQLRALEWI
                     GKWSMHDVLVLSLSIFFIKSQGVYDARSLNGVYFFTAAVLFMILAYAWLRGDVARDEV
                     AAASVVPLQRSQPPSTLRTFAFSFVIILATVFFALGVMLPAIRFTTVFVWTNQHSIAT
                     IIWALYKNEEFFLCFVIFMFSIFFPFLKLFYLLTLVTSPDMPAEFRDKSISAMEWLGR
                     YSMTDVMVLALMIFYINASGYTEASVLPGIYFFAASTLMTMLAYGWANSVTPGVRSPK
                     GPASLQARLAELSPGSRGPQKLAS"
     gene            complement(9134..9502)
                     /locus_tag="VFR00_05675"
     CDS             complement(9134..9502)
                     /locus_tag="VFR00_05675"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU0158777.1"
                     /translation="MPDAKLAGALILLIEDEPLIALDLSASLRDAGARVVQAANVEGA
                     LAAIEAHACSAAVLDFWLGLETGRAVARRLKEKRIPFLYYSGRALDDLTTGSGAPVIS
                     KPAGAREIVGMLRLLIDGRA"
     gene            9723..10232
                     /locus_tag="VFR00_05680"
     CDS             9723..10232
                     /locus_tag="VFR00_05680"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HEU0158778.1"
                     /translation="MTRKTGASTVALLLVLGLTPPALAQTTQPSQQAPKATEAPKAPV
                     TGQIVTQEDNTILAKDLIGQTVYAPDKTKIGSISDLILSKDGKTVQGFVIGVGGFLGI
                     GEKSVAMKIDRLQIAQDKDGMQLTMDAKKEELANAPSFKSKKDVDAERQSAERASNPP
                     TGPRPTTKP"
     gene            complement(10305..>10760)
                     /gene="rpsD"
                     /locus_tag="VFR00_05685"
     CDS             complement(10305..>10760)
                     /gene="rpsD"
                     /locus_tag="VFR00_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011088452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEU0158779.1"
                     /translation="KQKLKGYYANITERQFNGIYQAARRFKGSTSEHLIGLLERRLDA
                     IVYRAKFVPTMFAARQFVSHGHVTVNGRRVNIASYRCRVGDVIEIKEKAKQLTLLLEA
                     IKSAERDVPDYIEADHNRMTAKLTRIPGLADVPFPVHMEPNLVVEFYSR"
BASE COUNT         1773 a         3514 c         3533 g         1940 t
ORIGIN      
        1 catgcgcgag cagctgatcc gcgaggtgga gatcattcct ctcgaggtgg tggtgcgcaa
       61 tgtcgcggcc ggctcgctgg cacagcgcct cggcttggaa gagggcaagg cgctgccgcg
      121 ctcgatcatc gagttttact ataaaaacga cgcgctcggc gacccgatgg tgtcggagga
      181 gcacgtcacg gcgttcggct gggcaacgcc gccggaaatc gacgagatca tggcgctggc
      241 gatccgcgtc aacgattttc tggttggatt gttttcaggc attggcatcc gcctcatcga
      301 tttcaaggtg gaattcggcc ggctctggga aaacgagcag atgcgcgtcg tgctcgccga
      361 cgagatcagc ccggattgct gccggctgtg ggacgccgcc acctccgaca aactcgacaa
      421 ggatcgcttc cgccgcgatt tgggcggcgt gctcgaagcc tatcaggaag tggcgcggcg
      481 cctcggcgtc ctgacggaga acccgcgccc gaagggctcg ggcccggtgt tggtgagctc
      541 caagccgcat tgacggaccg gggcggctgg ggcatgtggc cggcgagcgg cttgggcacg
      601 ggcccgccgc ctgagcgagg gcatggcgtt tgagcatgaa ggcgcgcatc aagatcacac
      661 tgaaaggcgg ggtgctcgat ccgcagggca aggcgatcga gggggcgctt gccggcctcg
      721 gcttcacggg cgtgagggag gtgaggcagg gcaaatacat cgaggtcgat ctcgccgaga
      781 gcgacgaggt gcgcgcccgc gaacaggtgg agcgcatctg caaggagctg ctcgccaaca
      841 ccgtgatcga aaactacgcc tacgagctgt cacgatgaga aaacctcggc ggctggccgg
      901 cggctttgag cgcatggtcg cgctggcgcg gttcagcctg gcccccctgg cctgcttgct
      961 tgctgcgctg ttggcgccgc tggcggccca gcagatacca gcgtccatgt tgacggtcga
     1021 aagcaccgcc ccgcatcgct atcaggtcac ggtgccggcc ggctgccggc aggaggaagg
     1081 accagggacc ctcgatgcga tctgctcggc cgaattcgac gccgaaaaga gcgccgaggc
     1141 aagcgcggcg gcctcgttgg tgttcgaggt cggcgccgaa gcggtggccg gcgacgccga
     1201 taaggccccc tcccaactcg ccgccggtta tggcgaggac gtcttcaaga gcgagttgcc
     1261 ggaggcggtg tgcggggagg ccgaccgggc gcgcgtgaag atcgagaacg tgaagcaggt
     1321 tctcgaagag gcacgagtcg cctacactgc cgatgtgctc tgctccgagg tcaagtttct
     1381 gggcctgagc gagcgccgtg ccagcgtcaa agtcgtggtc acgcccgggg tgcgctatcg
     1441 cctgctggcg cgagctccgc agggcgagta cgagcagcac aagcacttga tcgaggcttt
     1501 tttcgccagc ttccgggtgc tacccaccgg ttaggtcgag cccatgcacg ccgccgtcat
     1561 cgtctttccc ggctccaact gcgaccgcga cgtcaaggtc gccctggaaa aggtaaccgg
     1621 gcaccccgtg tcgctggtct ggcacggcga tgccgcggta ccggacagcg atctcatcgt
     1681 gctgccgggc ggcttcgcct acggcgacta tctgcgcacc ggtgcgatgg ccgcccactc
     1741 gccgatcatg cgtgacgtgg tggccaaagc caaagcgggc acgcccgtgc tcgggatttg
     1801 caacggcttc caagtgctca ccgaggcggg actgttaccc ggcgtcctga tgcgcaacgc
     1861 ctccctcaag tttgtctgca aagacgtcta cctgcgggtc gagcagcccg acacgccgtt
     1921 cacggcgcgg tatcgccagg gcgaggtgat tcgcattccc gtggcacacg gcgacggtaa
     1981 ctatttcgcg gacgccgcga ccctcgatgc gctggaaggg gaaggccgcg tcgtctttcg
     2041 ctacgtcgat gcgggcggga accgcagcct cgaggccaat cccaatggcg cgcagcgcga
     2101 catcgccggc atctgcgatg cgcgccggcg cattctcggc atgatgccgc atcccgagcg
     2161 cctgttcgaa cctgcgcttg gcggatgcga cgggcgcggc gtgttcgaaa gccttctcgc
     2221 cagcctcaat tgaacgccgc tggcgcgcgg ccctcagccc atctttgctg ctcggggctt
     2281 tgcgcgcgcg cgcgcgcgcc ccatgtgatc gcgccggcaa ttgcggctat agtgggcggc
     2341 ctcacgtgcc aagggtttgc ggggaacgcg ctcgcgccag aggcccacag cctttaagag
     2401 gaggcggcag tcgcccctgc cccagtgcaa ccatcctccc tgccgccggg gacactgaca
     2461 acactaggaa gcccgatgca gaagcctgtc cctggagcgg agcggcgcaa ggcggcgatc
     2521 gccttcattt ttgccatcgt gctcctcgat gtgatcgccc ttggcatcgt cattcccgtg
     2581 ttgccgaagc tggtggagag catggtcggg gaaaccgcgc ggggcgcgga gatcttcggc
     2641 ctgttcggca cggcctgggc cctcatgcag ttcctgtgct cgccgctgct gggcgccctc
     2701 tccgaccgct tcgggcgccg ccccgtgctt ttgatttcga tggcaggaca cggcctcgac
     2761 tacattttga tggcgctggc gcccaacctg ctgtggctgt tcgtcgggcg cgtcatttcc
     2821 ggcattaccg ccgccagctt cggcacggcc tacgcctaca tcgccgacgt caccacgccg
     2881 gaaaagcgcg cccagagctt cggcgtggtc ggcgccgcct tcggcgtcgg cttcgttttg
     2941 gggccggccc ttggcggcat cctgggcgcc tacgatccgc gcctgccctt ctgggttgct
     3001 gccgccttca gcctggcaaa cgcggcctat ggctatcttt tcgtgcccga atcgctgccg
     3061 gaggaccgac gcatggcctt cagcctggcg cgcgccaatc cggtgggctc gctgcgcctg
     3121 ctgcggcaac gccccgagct cttgggcctg gcgggcgtga acttcctgca ccaactggca
     3181 cacgcctcgc tgcccgccat cgccgtcctc tacacctcct accgctacgg ctgggacagc
     3241 cagaccatgg gcctatcgct ggcggccatt ggcatcatgt ccggcctggt gcagggcacg
     3301 ctgatcaagc cgatcgtggc ccacgtcggg gagcggtgga ccttgctggc cggcctcctg
     3361 ttcggcgccg cgggctttgc catctatggc tttgcccgca cgagcgtgga gttctgggcg
     3421 ggaattccgg tgatggcgct atggggcatc gcgggcgctg ccacccaggc gatgatgagc
     3481 cagctcgtca ccccgtcgga gcagggccaa ctgcagggcg ccaacgccag catcatggcc
     3541 gttgccggtc tttttggccc cggcctcttc agtttcacct ttgcctggtc gatcgcgcgc
     3601 ggggaggccg cgggctggcc aggcctggcc ttcctgctgg ccgcggcctt gctcgtcttg
     3661 gccaccgcgc tcgcctggca ggtgacgcgg acggccgagg tgagcgagcc cggcgccaac
     3721 tagcatgacg ggtgctgcgg atctctgctt cgtcatctcg gcggactacg cgcgccggac
     3781 cggcggctgg atctatgatc acaggctgct gcaggacctc aggggacggg gttggcgcat
     3841 tgaagaggcc attctcccgg ccggcttccc gcagccatcg ccaacggcgc gcgcgcgcgc
     3901 cgccgcggtc ttccgcgcca tcccggacgg ccgcgtcgtg ctggtcgatc aactctgcct
     3961 cggcgtcctg cccgaagtgg cccgcgccga aggccggcgc ctgcgcctcg tcatgatcgt
     4021 gcaccacccg ctcgcgctcg agggctacgc cagcgccgag cagcgcgcgc gcgtgcagcg
     4081 cgcagaaaag gaggccctgg gccaggtggc ggcggtgatc gtcagctcgc cgaacacggc
     4141 gcgggctttg atgctggact acgcggtcag cgccgatcgc atcatcgtcg ccatccccgg
     4201 catcgaccgt cgagccctgg cgcgcggctc cggcgggcgg gtcctcaatc tactttcggt
     4261 tggcgcgctc gtgccgcgca aggaccatgc cctgttgctg cgggcactgg ccggtctgcg
     4321 gcatttgcct tggcggctgc agctggtcgg caatgtcacg cgtgcgcctc aacacgtcgg
     4381 gaggttgcgg ggcatcatcg gggcctgcgg gttagcgggc cgcgtcagcg tgcgaggcga
     4441 actccccgag catcgcctgc ttcggctctg gctgggcgcc gatctttatg tttcggcctc
     4501 gcgccatgaa ggctacggca tggcgctcgc cgaagccttt gcccatggca taccggtggt
     4561 gagcacctat gcgggcgcag cgggggcgtg ggtcgggcgc cgcggcgcca ggctcgtgcg
     4621 cggcaatagc gttgcctcgt tgcgcgcggc gcttggcgag gtgcttggca gctcggctcg
     4681 acgggcggcg ctgcaggtcg gcgcccgcgc gcgcaggccg agcctgccgc gctggcaggc
     4741 gacggccggg agcgtcgata ggcggctgcg acagctgctc gagagccgtt cataatcgct
     4801 ggtggcgacg cgacaagcgc gcgccagccg gctataagcg cgcaccacgg tttttgccct
     4861 ttccgccgcg agcaccatgc acgagcagag caaagcacgt ttcagccccg aggtcattgc
     4921 cgagcacggc ttcaaaggcg atgagtatca gcgcctgctg cagatcctcg gacgcgaacc
     4981 ctcgctgacg gaacttggca tcttttccgt gatgtggagc gagcattgct cctacaaatc
     5041 ctcgcgccgc tggttacgcg agctgccgac caaggggccg gcggtcattc aaggcccggg
     5101 tgagaatgcc ggcgtggtcg atctcggcga tggcgactgc gccgtgttca agatggaaag
     5161 ccacaatcac ccctcctaca tcgagcccta ccagggcgca gccaccggtg tggggggcat
     5221 catgcgcgat gtcttcacca tgggcgcgcg gccggttgcc aacctcaacg ccttgcgctt
     5281 cggcgccccg gcgcatccca aaacgcgtca ccttgttgcc ggcgtggtgg cgggcatcgg
     5341 tggctacggc aattgcacgg gggtgcctac ggtcgggggc gagacgaatt tcgacgcggg
     5401 ctacaacagc aacattctcg tcaatgccat gtgcgttggc ctcgccaaga cgcacaagat
     5461 cttttactcc gcggccaagg gtgtgggcct gccggtggtc tacgttggct ccaagaccgg
     5521 acgcgacggc atccacgggg ccacgatggc ctcggcagaa ttcgacgaca agtccgacga
     5581 gaagcgcccg accgtgcagg tcggtgatcc cttcaccgag aagctcttga tcgaagcctg
     5641 ccttgagctg atggcaagcg atgccatcgt cgccattcag gacatgggtg ccgccgggct
     5701 gacctcgtcg acctcggaga tggccgataa aggcggggtc ggcattgagc tcgatctcga
     5761 ccaggtcccg cagcgcgaga ccggcatgag cgcctacgag atgatgctct cggagtcgca
     5821 agaacgcatg ctgatgatcc tgaacccggg acgtgagcca gaagcgcagg cgatcttcaa
     5881 aaagtgggga ctggacttcg ccgtgatcgg ctcgaccacg gatagcggcc gcttgatcgt
     5941 caagcatcga ggcgtggtcg aggccgattt gcccgtcgac aagctcgcca actccgcgcc
     6001 gctgtacgag cggccctacg tcgagaccgc cccgccgcgc gccatcctgc ccgagtgggt
     6061 acctgcgccg aacactattc ttggcacctt gaagacgatg ctgggctccc cgcagctggc
     6121 ttcgcgccgc tggatctggg agcagtatga ccacatggtg atgggcgata ccgtgcaacg
     6181 tccgggcggc gatgccgccg tcgtgcgcgt gcacggcacc agcaaagggc tggcaatgac
     6241 gtgcgacgtg accccgcgct acgtcgccgc cgacccgcgc atgggcgcca agcaagccgt
     6301 ggcggagagc tggcgcaacc tgattgcggt cggcgccgac ccgatcgcca tcaccgacaa
     6361 cttgaacttc ggcaaccccg aacgccccga ggtcatgggg cagcttgtgg gagcgatcaa
     6421 aggcatgcga gaggcgtgcg aggccctgaa atatccggtc gtttcgggca atgtctcgct
     6481 ctacaacgag accaacggca tcgccatccc gcccacgccc gccatcggcg gcgtcggcct
     6541 catccccgac atcggcaaga tggccagcat cgccctgaag accacggggg atctgttgct
     6601 cgcggtgggc cgcgaggccg ggcatctcgg gcaatccctc tatcagctca gcatcaccgg
     6661 caagctcgaa ggctcgcctc cccccgtcga tcttgccgac gagatcaagg ccggcaacct
     6721 tgtgcgcagc ctgatccgcg agggcaaagt gcgtgccgtg catgacgtca gtgacggcgg
     6781 cctgctgacg gccgtggcgg aaatggcgct ggccggatcg ctgggcgtgc agctttatcc
     6841 ctacgaaggg cggctgcccc agcacgccgc ctggttcggc gaggaccagg gccgctacgt
     6901 cttggcggcc gatccggagc tggccgagga gataattgaa cgatcgcggc tacttgcgct
     6961 acccgctcgg gtgatcggcc gcgtcggtgg cgaagctctg gtcctcaagg aagaggccgt
     7021 tttgccgctg gctgagctgc gcgccgccca tgaggactgg ctgccgcgct acatgcgcgt
     7081 gccttgaggc cgccgccggg aggccataag cgccgccttt tgcgagcttg cccttgcttt
     7141 gggcttgtgg tgttgctggc tgaggcgcac ctatcatagg ctcaaccgta cgcttggctc
     7201 cggagacgtg cctgatgccc atggattccc aagacatcgc ccgcctgatc aaggcgcgct
     7261 ttcccgacgc ggaggtgacc attagggatt tggccggcga tggcgatcat tacgccgccc
     7321 acgtcgtgag caaggaattc aggggtaaga cgcgggtaca gcagcacaag atggtctacg
     7381 aggcgctgga gggccggatg ggcggggtgt tgcacgcctt ggccctgacc accgcccccc
     7441 gaccagattg actttgcccg ttgattgacc acatctaacc cttgagtacc tgggcgcgat
     7501 cgccgcccgc cacgagaggc aagagaggac ttttttgcat gagcgagcaa gccgtccacg
     7561 actggatccg caaggcggtc gccagcaacg atgtggtcct gttcatgaag gggaccaagg
     7621 attttcccca gtgcgggttc tcctcgcagg tcgcccaaat acttggccac ttgggcgtca
     7681 cctacaagga cgtgaacgtg ctggacgaca tgggcatccg cgagggcatc aagagcttct
     7741 ccagctggcc cacgattccc cagctctacg tcaaagggga gttcgtcggc ggctgcgaca
     7801 tcgtgcgtga gatgttccag gaaggcgagt tgcagcagct cctggatgcc aaaggcgtca
     7861 actacaatcg cgccaaggcc acggtttaag cgcgccgcac gccggcaccg cctcaggcag
     7921 gcgggccttg ctcggacata atcccgccgc ctcacccgct aggatggggc ggctttcgtt
     7981 cgagtatgag cgcgcatcgg catgacgctc ggcggccgcc ggtttctact gacccttgcc
     8041 atcgtcggtg ccagcgtctg cttggcgctg ggcgtgagcc tgccaatcat caaactcacc
     8101 aagtttgtct tcttcaccta cgagcactcc ctgatttcga ccgtgaatgc gctgatccgc
     8161 tccggacaga tctttttggg catcacggtt ctcatcttct cgattgcgct gccgatcctg
     8221 aagctcctct acctgttgct gctgtcgacc ttgcccaagc gcgaactgca gcgcctgtcc
     8281 cggcaactgc gagcgctcga atggatcggc aagtggtcga tgcacgacgt cttggtgctg
     8341 tcgctgtcga tcttcttcat caaaagccag ggcgtctatg atgcccgcag cctcaacggc
     8401 gtttacttct tcacggccgc cgtgctgttc atgatcctgg cttacgcctg gctcagaggc
     8461 gacgtggcca gggacgaggt cgccgcagcc tcggtggtgc cgctgcaacg gtcgcagccg
     8521 ccctcgacgt tgcgcacctt cgccttcagc ttcgtcatca tcctggcgac ggtgttcttc
     8581 gcgctcggcg tcatgctgcc cgccattcgc ttcaccacgg tctttgtctg gaccaaccag
     8641 cactccatcg ccaccatcat ttgggcgctt tacaagaacg aggagttctt tctctgcttc
     8701 gtcattttca tgttctccat cttttttccg ttcctaaaac tgttctacct cctgacgctg
     8761 gtgacctcgc ccgacatgcc cgccgagttc cgcgacaagt cgatctccgc catggagtgg
     8821 ctgggccgct attccatgac cgacgtcatg gtccttgcgc tgatgatttt ctatatcaac
     8881 gcctccggct acaccgaggc ctccgtgctc cccggcatct actttttcgc ggcctcgacg
     8941 ctgatgacga tgctggccta cggttgggcg aactcggtaa cgcccggcgt tcgctcgccc
     9001 aaggggccgg ccagcttgca ggcacggctt gccgagctgt ccccgggcag ccggggaccg
     9061 caaaaactcg cctcctgagg atgcccgcgc cgcaggtctc cgccggagcg gatgtgccga
     9121 ggcgccatgg ctctcacgcg cggccgtcga tcagtaggcg cagcatgccc acgatttccc
     9181 gcgcccccgc cggtttagag atcaccggcg cgccgctgcc cgtggtgaga tcatcgagcg
     9241 cgcggccgct gtagtagaga aacgggatgc gcttttcctt gagccgccgc gccacggccc
     9301 gcccggtttc caaaccgagc cagaaatcga ggacggcggc cgagcaggcg tgcgcctcga
     9361 ttgccgcgag cgcgccctcc acgttagcgg cctgcacgac acgcgcgccg gcatcgcgca
     9421 gcgaggcgct gaggtcgagc gcgatcagcg gctcgtcctc gatcaacagg atcagggcac
     9481 ctgcaagttt cgcgtccggc aaaagttccc ccacgtctcg gcgcggcagc cctgtgagac
     9541 gcgcgcgcct gatccagatt gaagaaaatt caccgcccgg caggaaaaag caagtctatg
     9601 tcacagcagt gataatcgcc gcctgggcgg cggcccgggc agcttgggca gaacctttcg
     9661 ggaagcgcgg cgttgtcccg atgaatgggc ctttagggct cgctatttcg tggaggttta
     9721 cgatgacgcg caagacagga gcgagcacag tcgctttgtt gctggtgctt ggcttgacgc
     9781 cgccggcact ggctcagacc actcagccat cgcagcaggc gccgaaggca accgaagcgc
     9841 cgaaggcgcc ggtgacgggt cagatcgtga cacaggaaga caataccatc ctcgccaagg
     9901 atctgatcgg tcagaccgtc tacgcgcccg acaagaccaa gatcggcagc attagcgatc
     9961 tgatcctcag caaggacggc aagaccgtac agggcttcgt gatcggcgtc ggcggcttcc
    10021 tcggcatcgg cgaaaagagc gtggcgatga agatcgatcg cttgcagatc gcccaggaca
    10081 aggacggcat gcagctcacc atggacgcca agaaggagga gctcgccaac gcgccgtcat
    10141 tcaagtccaa gaaggacgtg gacgccgagc gtcagtccgc tgaacgggcc agcaacccgc
    10201 cgacgggccc gcgtccgacc actaaaccat aacgatcgaa gcgcgctcaa tgctcgcggc
    10261 cggcgaggct ctcgccggcc gctttttttg gccgggctgc cctcttagcg gctgtagaat
    10321 tccaccacga ggttgggttc catatgcacg gggaagggca cgtcggcgag gccgggaatg
    10381 cgggtgagct tggcggtcat ccggttgtga tcggcctcga tatagtcggg cacgtcgcgc
    10441 tcggcgctct tgatggcctc gagcaggagc gtcaactgct tggctttttc cttgatctcg
    10501 atgacgtcgc cgacccggca gcgatagctg gcgatattga cgcgccggcc attcaccgtc
    10561 acgtggccgt ggctgacgaa ctggcgtgcg gcaaacatgg tggggacgaa cttcgcccga
    10621 tagacaatgg catcgagccg acgctccagc agtccgatca agtgttcgga ggttgaaccc
    10681 ttgaaccgac gcgccgcctg ataaatgccg ttgaactgac gctcggtgat attggcgtag
    10741 tagcccttca gcttctgctt
//
LOCUS       DASTMT010000241         6656 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_5644644, whole genome shotgun sequence.
ACCESSION   DASTMT010000241 DASTMT010000000
VERSION     DASTMT010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 6656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6656
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5644644"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(86..1168)
                     /gene="mraY"
                     /locus_tag="VFR00_05690"
     CDS             complement(86..1168)
                     /gene="mraY"
                     /locus_tag="VFR00_05690"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013945977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEU0158780.1"
                     /translation="MLYALVQFSDQIGPLNVFRYITFRTGGAIMTALLFVFLFGPAII
                     DLLRVKQGKGQPIREDGPQSHLAKKGTPTMGGLMILSGITVSTLLWASWANPYVWIVL
                     FVTLSYGAIGFYDDYLKVTRSQTAGFSTRLRFLAEVLVAALAAWAVMRMGSAGFSSSL
                     TVPFFKNVVIPLGFFFVLLGVLVIAGAGNAVNLTDGLDGLAIVPVMIAAATFGLIAYL
                     SGNANFARYLQIHYVQGAGELAVICGALIGAGLGFLWFNAPPAMIFMGDTGSLALGGA
                     LGAIAVATKHEIVLAIVGGLFVLETLSVIIQVSSYKLTGKRVFRMAPLHHHFEQKGWQ
                     ESTVVVRFWIISVVLALVGLATLKLR"
     gene            complement(1172..2587)
                     /locus_tag="VFR00_05695"
     CDS             complement(1172..2587)
                     /locus_tag="VFR00_05695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011509792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoylalanyl-D-glutamyl-2,
                     6-diaminopimelate--D-alanyl-D-alanine ligase"
                     /protein_id="HEU0158781.1"
                     /translation="MPEPLWTWSELAAAAQGTAEGQPAWPIRGLSIDSRSLRPGEVFV
                     ALSDKRDGHEFVPAAFRAGAVAAIVARAYRPSGSFPALLRVDDPLQGLINIARAARLR
                     SAARIIAITGSVGKTGTKEMLSLCLSELGGTHAAEKSYNNHWGVPLTLARLPRETEYG
                     VFEIGMNHAGEIAPLAALVTPHEALITSVEAVHLGHFNSVAEIAEAKAEILTGLVPGG
                     PAILPRDNPHFRLLRERAAALGARIVTFGYHQDADFRGLQVDLGPKGSSVIAGFGTQR
                     FPYRLGAAGEHYVKNSLAVLAALSALGVDIMKSLPALARVSPPAGRGARTPLETAEGP
                     ILLIDESYNANPASVRAALAGMATTPRESYPRRVAVLGDMLELGQTAPDLHRGLKDAI
                     DAAGVDLVLACGPLMRLLHESLSAGQRGAWAETSVELVDRLLATVRAGDVVMIKGSLG
                     SNMAPLLAALLTRFRPVRSGG"
     gene            complement(2580..4049)
                     /locus_tag="VFR00_05700"
     CDS             complement(2580..4049)
                     /locus_tag="VFR00_05700"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011426097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HEU0158782.1"
                     /translation="MRLQVLADGAADLEMSPNDGEVEILSLTADSRSVRAGALFAALS
                     GSKTHGTAFIADAIAQGAAAVLLKSGADVRLPPSVALLRAPEPRQAFARLAARFWGRQ
                     PSTVIAITGTSGKTSVAEFARQIFHALGHRAASLGTLGIVTASATRYGALTTPDPVSL
                     HQTLAQLAEEGITHLAMEASSHGLDQYRLDGIALSAAAFTNLGRDHLDYHPSEAAYLK
                     AKLRLLTELLPEAGTAVVAADATHAPSALAAARAAGRKVFTVGKAAGSTLQLEAQHPV
                     GFGQQLRVRHGAGACDLTLPLIGDYQASNALVAAALVMATGEPAEPTLAALEQLRGVK
                     GRLEIVGQARGGLIVVDYAHKPDALAAALQALKPFAAGRIVCVFGCGGDRDKGKRPIM
                     GRIASELAGSVIITDDNPRSEPAAAIRAEILAGAPAAREIGDRAEAIKAGVRLLGSGD
                     VLLIAGKGHETGQIIGSTTVPFSDHEAVRAALAEAPLNA"
     gene            complement(4119..5837)
                     /locus_tag="VFR00_05705"
     CDS             complement(4119..5837)
                     /locus_tag="VFR00_05705"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEU0158783.1"
                     /translation="MIRLLAAIPKRPRPMPMPVAASGRRPIRRRTLLALAALLACFVA
                     IAAQLLRLGLRQEADLHLNVAEPIGQSWSRPDIVDRNGQLLATDVASHSLYADPQLVL
                     DLDEVIEKVTAVLGGLDRAEVRRALADRSRRFTWIARGLSPRQAQLIHDLGLPGLAFR
                     TELKRVYPLGPLAGHLLGTVNVDNKGLAGLERWLDETAKVEAVHGPERSDKPALRLAL
                     DVGVQHALAEELQEAVRRYSASGAAGLVLDLTDGEILAAASLPGADPARPADWLDLRH
                     ADKLQGGSFELGSVFKTLTVAMALEAGVADLDTVYDVRRPLTIGAYTITDLHPSSRPL
                     SLREIFLHSSNVGAGLVALDLGADRQRQFLAKLGLLEPLRTQVGLSAAPQLPKHWGRI
                     ETVTISSGPGLAVAPLQLAAAMGAAITGAQLSPTLLAGEARPQPKPLLAPATSARVRE
                     ILRLNVTSPLGTGRRADAEGYRVGGKTGTAEMPGRGGYQAKAVISSFIGAFPMDAPKY
                     LTLVMLFEPEPVPGLAPGITAGLNAAPTTARIVHRIAPVLGVLPRPIGASDPREGPFD
                     ALTAAQ"
     gene            complement(5839..6123)
                     /locus_tag="VFR00_05710"
     CDS             complement(5839..6123)
                     /locus_tag="VFR00_05710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HEU0158784.1"
                     /translation="MLRIINSALLLLTLVAVFALYAIKYDARRLEARINMQERDLEKL
                     ENHVAVLVAERAHLARPKALEPLARNLGLAPIAPRQYLRLEAPPAADPGR"
     gene            complement(6151..>6656)
                     /gene="mraW"
                     /locus_tag="VFR00_05715"
     CDS             complement(6151..>6656)
                     /gene="mraW"
                     /locus_tag="VFR00_05715"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013945972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="16S rRNA
                     (cytosine(1402)-N(4))-methyltransferase"
                     /protein_id="HEU0158785.1"
                     /translation="AARAIARRILARRTERPLTRTGELAELVVRVLGREKIAGRHAAT
                     RTFQALRMHVNDELGELSRALAAAERCLQAGGRLVVVTFHSLEDRLAKQFLRLRATPA
                     AQASRHLPPGEAEHPAPSFRFINPRPVSPKAEEVAANPRARSAKLRAAVRTDAPAWPE
                     LVAGARD"
BASE COUNT         1160 a         2179 c         2331 g          986 t
ORIGIN      
        1 ctggtggccg gcgtcatggc gcaggcctag cgccgagagc cgcggtgcgg ggggcgtgga
       61 ttggctctcg caagggcgcc gtccctcagc gcagtttcag ggtggcgagg ccaaccaggg
      121 ccagcaccac cgaaatgatc cagaagcgca cgaccacggt cgattcctgc catccctttt
      181 gctcgaaatg gtggtgcagc ggggccatgc gaaacacgcg cttgcccgtc agcttatagg
      241 acgacacctg gatgatgacg gacagtgttt ccaacacgaa caagcccccg acgatggcca
      301 ggacgatctc gtgcttggtc gccaccgcaa tggcgccgag cgcgccgccc agcgccaagg
      361 acccggtgtc gcccatgaaa atcatcgccg gcggggcatt gaaccagaga aaaccaagcc
      421 ctgccccgat caaggctccg cagatgacgg cgagttcgcc ggcaccctgc acgtagtgaa
      481 tctgcaggta gcgcgcgaaa ttggcgttgc cggaaaggta ggcaatgagg ccgaaggtgg
      541 cggccgcaat catgacagga acaatggcaa ggccatcgag accatcggtg agattgacgg
      601 cgttgccggc tccagcgatc accagcacgc ccagcagcac gaagaaaaag ccgagcggga
      661 tcaccacatt tttgaagaag ggcacggtca gcgatgagga gaagccggcg ctgcccatgc
      721 gcatgaccgc ccaggcggcg agcgccgcca ccagcacctc ggccaagaac cgcagccgcg
      781 tcgagaaccc ggcggtctgg ctgcgggtga ccttcaaata gtcgtcgtaa aagccgatgg
      841 caccgtaact caaggtgaca aagaggacga tccagacata agggttggcc cagctggccc
      901 agagcaaagt tgagacagtg atgccggaca ggatcatcag tccgcccatg gtgggcgtgc
      961 ccttcttggc caggtgcgac tgtggcccgt cttcgcgaat aggttggccc ttgccctgct
     1021 tcacgcgcaa cagatcgata atggccggcc caaacaggaa cacgaacaag agcgcggtca
     1081 taatggcgcc gcccgtgcga aaggtgatgt agcgaaagac gttgagcgga ccgatttgat
     1141 cgctgaactg caccagggcg tagagcatgc gttagcctcc ggaccgcacc ggacggaacc
     1201 gcgtcagcag cgccgccagg agcggagcca tgttgctgcc gagcgaaccc ttgatcataa
     1261 cgacatcacc ggcgcgcacc gtcgccaaga gacgatcgac gagttctacc gaggtctcag
     1321 cccaggcgcc acgctggccc gcgctcaggc tttcgtgcag gaggcgcatc agcggcccgc
     1381 aggcgagaac gaggtcgacc ccggcggcat cgatagcgtc cttgagaccg cggtgcaggt
     1441 cgggcgcggt ctgacccaat tccaacatat cgcccaggac ggcgacccgg cgcgggtagc
     1501 tctcgcgcgg tgtcgtcgcc atgccggcca aggcggcgcg cacggaggcc gggttggcgt
     1561 tatagctttc gtcgatcagc agaattgggc cctcggcggt ctcgagcggc gtgcgcgcgc
     1621 cacggcccgc cggcgggctc acgcgggcca gcgcgggcaa gctcttcatg atgtcgacgc
     1681 cgagcgcgct caaagccgcg agcacggcca gtgaattctt cacgtagtgc tcgcccgccg
     1741 cgcccagccg atagggaaag cgctgcgtcc cgaagcccgc gatcaccgag gaacccttgg
     1801 gcccgagatc gacctgtagg ccgcggaaat cggcatcctg atgatacccg aaggtcacta
     1861 ttctggcgcc gagggccgcg gcgcgctcgc gcagcagcct gaaatggggg ttgtcgcgcg
     1921 gcaggatggc gggtccgccg ggcaccaatc ccgtcagaat ctcggccttg gcctcggcga
     1981 tctcggcgac agaattgaag tgaccgaggt gcaccgcctc gacgctagtg atgagggcct
     2041 catgcggcgt caccaacgcg gcgagcggcg caatctcccc ggcgtggttc atgccgattt
     2101 cgaacacccc atactcggtc tcgcgcggca gacgcgccag tgtcagcggc acgccccaat
     2161 gattgttgta ggatttttcc gccgcgtgcg tgccgccgag ctcgctgagg cacaggctca
     2221 gcatctcctt ggtgcccgtc ttgccgacgc tgccggtgat ggcgatgatg cgcgcggccg
     2281 agcgcaggcg cgcagcgcgc gcgatgttaa tgagcccctg caagggatca tccacgcgca
     2341 ggagcgcggg aaaggatccg ctcggccgat aggcgcgggc aacgatggcc gccacggccc
     2401 cggcgcggaa cgccgcgggc acgaactcgt ggccatcgcg cttgtcactg agggcgacga
     2461 acacctcgcc agggcgcaag ctgcggctat cgatcgacag gccacggatg ggccaagccg
     2521 gttggccttc cgctgtgcct tgcgcggcgg ccgccagctc actccaggtc cacaggggct
     2581 caggcattga gcggggcctc cgcaagggca gcccgcaccg cctcgtggtc ggagaagggc
     2641 acggtagtgg aaccaatgat ctggcccgtc tcgtggcctt tgccggcgat caacagcaca
     2701 tcgccgctgc cgaggagccg cacgccggcc ttgatcgcct ccgcgcggtc gccgatttcc
     2761 cgggcggccg gcgcgccggc caaaatctcg gcgcgaatcg cggcagccgg ctcgctccgc
     2821 gggttatcat cggtgatgat gacgctgccg gcgagctcac tggcgatgcg acccatgatg
     2881 gggcgcttgc ccttgtcgcg atcgccccca cagccaaaga cgcaaacgat gcgccctgcg
     2941 gcaaagggct tcagcgcctg cagcgcggcc gccaaggcat ccggtttgtg ggcataatcg
     3001 acgacgatca agccgccgcg cgcttgcccg acgatttcca ggcgcccctt gacgccgcgc
     3061 agctgctcca gcgccgcaag cgtgggttca gcgggttcac cggtggccat gaccagggcg
     3121 gcagccacga gcgcattgct cgcctgatag tcgccgatca gcggcaaggt cagatcgcat
     3181 gcgcccgcgc cgtggcgcac gcggagctgt tggccaaagc cgacgggatg ctgcgcctcc
     3241 agctgcagcg tgctgccggc tgccttgcca acggtaaaga ccttgcgccc ggcggcccgg
     3301 gctgccgcga gcgcggaggg cgcatgcgtc gcgtccgctg cgacgacggc ggtgccggct
     3361 tcgggtaaca gctcggtcag caagcgcagc ttggccttaa ggtaggccgc ctccgaggga
     3421 tgatagtcga ggtgatcgcg gccaaggttg gtgaaggcgg ccgcgctgag cgcgatgccg
     3481 tccaggcggt attggtcgag gccgtgggat gaggcttcca tggccaggtg cgtgatgccc
     3541 tcctcggcga gctgggccaa ggtttgatgc aggctcacgg ggtcgggggt cgtgagcgcg
     3601 ccgtaacgcg tcgcgctcgc tgtgacgatg cctaaagtgc cgagggatgc ggcccggtgt
     3661 ccgagggcat gaaaaatctg ccgggcaaac tcggcgaccg aggtcttgcc acttgtcccc
     3721 gtaatggcga tgacggtgct gggctgccga ccccaaaagc gggcggctag gcgcgcaaag
     3781 gcttgccggg gctccggcgc gcgcaggaga gccacgctgg ggggaagcct gacatctgcg
     3841 ccggatttga ggagcaccgc ggcggcgcct tgagcgatgg catcggcgat gaaggcggtg
     3901 ccgtgcgtct tgctgccgga caaggccgca aacagggcgc cggctcgcac gctccggcta
     3961 tccgccgtca gcgacagaat ctcgacctcg ccatcgttcg gcgacatctc aagatcggcg
     4021 gcgccgtcgg ccaaaacctg gagcctcatt gcactcgacc ctaatccttg cgcgggccgg
     4081 gtgcgcccgc gcgctccccc gcagataagc ctcgcttctt attgcgccgc agtgagggcg
     4141 tcaaacgggc cctccctggg gtcactggcc ccgatcgggc gcggcagaac ccccagcacc
     4201 ggggcgatac gatgcacgat tcgcgccgtc gtcggggcgg cattgaggcc ggcggtaatg
     4261 cccggcgcca gccccgggac cggctcgggc tcaaacagca tcaccagcgt caggtacttg
     4321 ggcgcatcca tgggaaaagc gccgatgaag gaggagatca ctgccttggc ctgatagccg
     4381 ccgcggcccg gcatttccgc cgtgccggtc ttgccgccca cgcggtaacc ctccgcatcg
     4441 gccctgcgcc cggtccccag gggtgaggtg acgttgaggc gcagaatttc gcgaacgcgg
     4501 gcgctggtgg ccggcgccag cagcggcttg ggttgcggcc gcgcctcgcc ggcgagcaaa
     4561 gtcggggaaa gctgggcgcc ggtgatggcg gcccccatcg ccgcggcaag ctggagcggc
     4621 gccaccgcaa ggccggggcc ggagctgatg gtgacggtct cgatccggcc ccagtgcttg
     4681 ggaagctgtg gcgctgcgct caagcccact tgcgtgcgca agggctcgag caaccccagc
     4741 ttggccagga actggcgctg gcgatccgct ccaaggtcca aggcgacgag gccggccccg
     4801 acgttggagg agtgcaaaaa gatttcgcgc aggctgagag ggcgcgagct tgggtgcaga
     4861 tcagtgatgg tataagcccc gatcgtcagc ggccggcgca catcatagac cgtgtcgaga
     4921 tcggcgacgc cggcctcgag cgccatcgcc accgtcagcg tcttgaaaac cgagccgagt
     4981 tcgaagctgc cgccctgcag cttgtcggcg tgccggaggt cgagccagtc ggcgggtcgc
     5041 gccggatcgg cgcccggcaa ggaggcggcc gccagaattt ctccgtccgt cagatcgagc
     5101 accaaacccg ccgcgccgct ggccgaatag cggcggaccg cctcctgcag ctcttcggcg
     5161 agggcatgct ggacgccgac atcgagcgcc aggcgcagcg caggtttgtc gctgcgctcg
     5221 ggtccgtgca cggcttcgac cttggcggtc tcatcgagcc agcgctcgag ccccgccagg
     5281 cccttgttgt cgacattgac ggtgcccaac aggtgacctg ccagcgggcc caagggatag
     5341 acgcgcttga gctcggtgcg gaaggcgaga cccggtagtc ccaggtcatg gatcaactgg
     5401 gcctggcgcg ggctgaggcc gcgcgcaatc caggtgaagc gccggctcct gtccgccaag
     5461 gcgcgacgca cctcggcgcg atcgaggccg ccgaggacgg cggtgacctt ctcaataacc
     5521 tcatcgaggt cgagcacgag ctgtgggtcg gcgtagagcg aatggctggc aacatcggtg
     5581 gccagcagct gcccattgcg atcgacaatg tcgggccgtg accagctctg gccgatcggc
     5641 tcggcaacgt tgagatggag gtcggcctcc tgccgcagtc caagcctcag cagctgggcg
     5701 gcgatagcca caaagcaggc gaggagagcg gccagcgcca gcaatgtgcg ccgccggatt
     5761 ggcctgcggc cggaggcggc gacgggcatc ggcatcgggc gcgggcgttt gggaatggca
     5821 gcgagaagcc gtatcatctc atcgccccgg atcggcggct ggcggggctt cgaggcgcag
     5881 gtactggcgc ggcgcgatcg gcgccaggcc caggttgcgc gcgagcggct cgagcgcctt
     5941 cggccgggcc aggtgggctc gctcggccac cagcacggcc acatgatttt ccagcttctc
     6001 cagatcgcgc tcttgcatgt tgatgcgggc ctcgagccgg cgcgcgtcgt atttgatggc
     6061 gtaaagtgca aacacggcaa ccagcgtcag cagcagcagt gccgagttga tgatgcgcag
     6121 catgtgcggt cccgaccttg tcagcagcaa ctagtcgcgg gcaccggcga cgagctctgg
     6181 ccaggcgggc gcatcggtac gcaccgcggc gcgcagcttg gccgaacggg cgcgcgggtt
     6241 ggccgcgact tcctcagcct ttgggcttac ggggcgtggg ttaatgaatc gaaaactggg
     6301 cgccggatgc tcggcctcgc cggggggcaa atgccgagac gcctgcgccg cgggcgtcgc
     6361 ccggaggcgc aaaaactgct tggccagacg atcctctaag gagtggaagg tgacgacgac
     6421 gagccgcccc cccgcttgca agcagcgctc ggccgccgcc aaggcacggg agagctcgcc
     6481 cagctcgtcg ttcacgtgca tgcgcaacgc ctggaaggtg cgcgtggcgg cgtggcgacc
     6541 ggcgattttt tcccggccca gcacgcgcac cacgagctcg gccagctcgc ccgtgcgcgt
     6601 caggggtcgt tcggtccggc gggcgaggat ccgccgcgcg atggcgcgtg cggcgc
//
LOCUS       DASTMT010000242         2494 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_128354, whole genome shotgun sequence.
ACCESSION   DASTMT010000242 DASTMT010000000
VERSION     DASTMT010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2494)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2494)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2494
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_128354"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(43..456)
                     /locus_tag="VFR00_05720"
     CDS             complement(43..456)
                     /locus_tag="VFR00_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018098299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0158786.1"
                     /translation="MQRASFGELLDALARADPDGGKPPAAARHRHAFVVETSGPAADR
                     TPPDSQRLLESLYGSETVPEASEADALVALHSWVVDELQLTADMTAGELQAVRRRFAR
                     AHHPDRAPAAERALASRRMMIANTLIDAALARRAS"
     gene            633..1745
                     /locus_tag="VFR00_05725"
     CDS             633..1745
                     /locus_tag="VFR00_05725"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:NF005085.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HEU0158787.1"
                     /translation="MPASAPALRAEVIGSLLRPRTLKDAVAAHTRGALPRAEVEAVLA
                     REVARVIARQEALGFRVVTDGEFGRTSWFGFFFEGMQGFRLAPSLFQFKDAMGQAFPW
                     QTCMAVEPIARRGPITLPEYQRARRYAETALVKVTMPAPSAFHFFRLDAAADPAVYPD
                     RQGYFGDLVGVYASELRELAAAGCRYVQFDEVPLAMLCDPSVREQAAGQGADPYALLD
                     TYIGVIAQICAQRPAGMRLGLHVCRGNFRGRWMAAGGYEPVADKVFNGVPVDVFLLEY
                     DSERAGDFTPLRFVPEGKQVILGLLSSKLERLEEEAELLRRIEAAARVLPLERLGLST
                     QCGFASVAGGNELPEAAQWAKLERVARTAARVWGTL"
     gene            2006..>2494
                     /locus_tag="VFR00_05730"
     CDS             2006..>2494
                     /locus_tag="VFR00_05730"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU0158788.1"
                     /translation="MNIAPSTRDMAAGHDVTAAAVQLSIEAVSKRFGGVAAVDGVSID
                     VPKGRILSIIGPNGAGKTSLLNLVSGFYKPDRGRVIFKGRDVTRLSVHQRAALGLGRT
                     FQNVGLVKGSTVRENLLTAQYLEADYSTLSGMLGAPRTFSGERELRQRADAIAEVMGL
                     GDI"
BASE COUNT          360 a          799 c          900 g          435 t
ORIGIN      
        1 cctttgccta aaattgtatc gatcgccgcg gccaggttct ctctagcttg cccggcgtgc
       61 gagcgccgca tcgatcagcg tgttggcgat catcatgcgc cgcgaggcca gagcgcgctc
      121 ggcagcggga gcgcggtcgg gatggtgggc gcgcgcaaac ctgcgccgca ccgcctgaag
      181 ttcgccggcc gtcatgtcgg ccgtgagctg cagctcatct acaacccaac tgtgcagggc
      241 gaccagcgcg tcggcctcgc tcgcttcggg cacggtttcg ctgccataga ggctctccag
      301 caggcgctgg ctatcgggcg gggtacgatc ggcggccggg ccgctggtct cgaccacgaa
      361 ggcatggcgg tggcgcgcgg cagccggcgg cttaccgccg tcgggatccg cccgcgccag
      421 ggcatcgagg agctcgccga aacttgcacg ctgcatgggt cttgggcccg aggcacgcgg
      481 agggacggcg gcagattggc ctgcgaaggt tgcgccgggg ttaagacgtg tcctggcgcg
      541 tgggtgccac gcgctgttgc cgggggctga ttgacagggc gccgatgccg cgggaggctc
      601 gcccagcaga ggacgggttc ggaggatcgg caatgccggc gagcgcacca gccttgcgcg
      661 ccgaggtgat cggcagcctg ctgcggccgc gcacgctcaa agatgccgtg gcggcacaca
      721 cgcgcggggc gctgccgcgg gccgaggtgg aggcggtgct cgcgcgcgag gtggcgcgcg
      781 tcatcgcgcg ccaggaggcg ttgggtttca gggtcgtcac ggatggggag tttggccgca
      841 cctcctggtt cggctttttc ttcgagggca tgcagggttt ccgtttggcc ccctcgctct
      901 ttcaattcaa ggacgccatg ggccaagcct tcccctggca gacctgcatg gcggtcgagc
      961 caatcgcgcg gcggggtccc atcacgctgc ccgagtacca gcgcgcgcgg cgttatgccg
     1021 agacggcctt ggtcaaggtc accatgccgg cgccgagcgc ctttcatttc ttccgcctcg
     1081 acgccgcggc cgaccctgcc gtctacccgg accggcaagg ctactttggg gatttggtgg
     1141 gcgtgtacgc gagcgagttg cgggagcttg ccgccgccgg ctgccgctat gtgcaattcg
     1201 acgaggtgcc gctcgccatg ctctgcgatc cgagcgtgcg cgaacaagcc gcgggccagg
     1261 gcgccgaccc ttacgctctt ctcgacacct acatcggtgt cattgcgcag atctgtgcgc
     1321 agcgtccggc gggcatgcgg ctcggcctgc atgtgtgccg cggcaatttt cgcggccgct
     1381 ggatggccgc gggcggctat gagcccgtgg ccgacaaagt cttcaacggc gtgccggtcg
     1441 acgtcttcct gctggagtac gacagcgagc gggccggtga cttcacgcct ttgcgcttcg
     1501 tgcccgaggg caagcaggtg atcttgggcc tcctgtcctc caaactcgag cgcctggaag
     1561 aggaagccga gctcctgcgt cgcatcgagg cggccgcgcg cgtcctgccc ctggagcggc
     1621 tgggactctc gacccagtgt ggatttgcca gcgttgccgg cggcaatgaa ctgcccgagg
     1681 cggcgcaatg ggccaagctc gaacgggtgg cgcgaaccgc cgcacgagtt tggggcacgc
     1741 tctagggttg gggggcagcg gcgaagcttt ttctcatcct ttggtagcgg gaagcgcatt
     1801 tgcgcccgct gggggcccct ggcgcggcgg aaattgctga attaacatcc aaccgccgac
     1861 gcggacgggg tcaccaacaa gagtggcggg tctgcggatc tgtcatgatc gcggcaaagg
     1921 gaggattccg ggatgtacgc tggccggtgc tctggctgcc tacgtgtcct ttgtcgctgc
     1981 aaggcaaggg catgaccatc gcgccatgaa cattgcgcca agtacccgcg acatggctgc
     2041 tggacacgac gtgactgcgg ctgcggtgca gttgagcatc gaggcggttt cgaagcgttt
     2101 tggcggcgtg gcggcggtcg acggcgtctc catcgacgtg cccaagggcc gtattctctc
     2161 catcatcggg cccaacggtg cgggcaagac ctcgcttctc aatctcgtct cgggcttcta
     2221 caagccggac cgcgggcgcg tgatcttcaa gggccgggat gtgacgcggc tctccgtcca
     2281 ccagcgcgcc gcgctcggcc tgggccgcac gttccagaac gtcggcctgg tgaagggctc
     2341 gacggtgcgc gagaacctgc tcaccgccca gtacctcgag gccgactact cgacgctgtc
     2401 ggggatgctg ggcgcgcccc gcacgttcag cggcgagcgc gagctgcggc agcgggccga
     2461 cgcgatcgcc gaggtgatgg ggctgggcga catc
//
LOCUS       DASTMT010000243         1503 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_3083258, whole genome shotgun sequence.
ACCESSION   DASTMT010000243 DASTMT010000000
VERSION     DASTMT010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 1503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1503
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3083258"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..879)
                     /locus_tag="VFR00_05735"
     CDS             complement(<1..879)
                     /locus_tag="VFR00_05735"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HEU0158789.1"
                     /translation="MAGLERAWLGSVPPMRHERHYGDRIMRCFLERPGNAFDLLAAAA
                     ARNPGGEAIVCGEERLSYAVFLESVEQCAAGLAAAGIGRGDRVAMLLGNGIAFPVVLF
                     ATLRLGAIAVPLSTREQRGGLAYMLAHAAARLLVYDTDLADRLPAPAATPDLAHRIAV
                     DPSASYRSLRLLAGACGAAPAGVEEEDTAIILYTSGTTGRPKGAMLTHLGICHSAMHY
                     EACMGLTARDRALAAVPLSHVTGVIALIAAMVRAAAALIVMPSFKAGEFLALAVREGM
                     THSLMVPAMYNLCLLEP"
BASE COUNT          256 a          502 c          508 g          237 t
ORIGIN      
        1 gggctcgagc aggcacaggt tgtacatcgc cggtaccatc agcgagtgcg tcatgccctc
       61 gcgcacggcc agtgccagga attcgccggc cttgaaactc ggcatcacga tcaaggccgc
      121 ggccgcccgc accatggccg cgatcagcgc aatcacgccg gtgacatggc tgagcggcac
      181 cgctgccagt gcccggtcgc gggcggtgag ccccatgcag gcctcgtagt gcatggcaga
      241 gtgacaaatg ccgaggtggg tcagcatggc gcccttgggg cggccggtgg tgcccgaggt
      301 ataaaggatg atggcggtat cctcctcctc gaccccggca ggcgcagccc cgcatgcgcc
      361 ggccagcaag cgcagggatc gataggaggc cgaggggtcg acggcgatgc gatgggcgag
      421 gtcgggcgtt gccgccggcg ccggcagccg gtcggccaga tcggtgtcat agaccagcag
      481 ccttgctgcg gcgtgcgcca gcatgtaggc gagcccaccg cgctgctcgc gcgtgctgag
      541 cggcaccgca atggcgccga gccgcagcgt ggcaaacagc acgacgggaa aagcaatgcc
      601 attgccgagc agcatggcaa cccggtcacc cctgccgatg ccggctgcgg ccaaccccgc
      661 ggcgcattgc tctacgcttt caaggaagac ggcgtagctc aggcgctcct cgccgcagac
      721 gatcgcttcc ccgccagggt tgcgggcagc ggcggcggcc agcagatcaa aggcgttgcc
      781 gggtcgctcg aggaagcagc gcatgatccg atcgccgtag tgccgctcgt gccgcatcgg
      841 cggcacgctg ccgagccaag cccgttccaa gccagccact ctttaagctc ctcgcgaccc
      901 ctatccgcaa aaaacgtatg ggacaccttg ccgtggcggg tggtcgtgtc aagggcacgg
      961 ggcgcactgg cgccgcttac ttgcctgccg tccacccgtg cagcgagctc ccgcggcaac
     1021 tggggcaatt cgatcccagc ctctcgctac gatttgtgcg ggatttgatt gcgatcatct
     1081 tgcacggggc gtgggtcaat gacatgacgt ttgccttcgg tgctgaaggc gcgcgcctag
     1141 gagcccgccc ccgtcaagcc gttttggctc accgaggcac gtggtcgggt cagaccgttg
     1201 cagtcgttgt gctcgagcac gtgcttggca acttgcacga ccagccgcac gggcccccct
     1261 catggagcga gcaagcgcca ctccgaaccc aagcatgggc ttcgttgggt ggagagagtt
     1321 gactggatcg acacttgagt tggtcaaacg gtgttgcgtg atgcgctttc actcacctgc
     1381 cgcagctgca cgaccgccct cagcatacgc gaccgtcgag cgagccctgc ggctggtcgc
     1441 agttggcgcg gaaaccgccg tcacccggat gggcggagac gccggctccc ggccaaggcc
     1501 cgc
//
LOCUS       DASTMT010000244         2546 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_5644992, whole genome shotgun sequence.
ACCESSION   DASTMT010000244 DASTMT010000000
VERSION     DASTMT010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2546
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5644992"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1096)
                     /locus_tag="VFR00_05740"
     CDS             complement(<1..1096)
                     /locus_tag="VFR00_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018640811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEU0158790.1"
                     /translation="MAPCQHWPSRRAALLGSGALFAWTQLPKLARAEGRDPRLLVIVL
                     RGALDGLGAVAPVGDPDWIGLRGDRALVLDGKTPALALNSFFALNPAMPNLYRLYQAN
                     AATIVHATATPYRERSHFDGQDVLESGFAKPGVYDSGWLNRALLALTPGGPVDPVGAR
                     ALAVGPVTPLVVRGRAPVMSWAPQLVVPASDDTRARLLELYQHTDLKLAAALEGRIRL
                     AALAHADGSTAAGPSPQLPPAGLARARAYFAEAAGTAARYLARPDGPRVGAMGFVGWD
                     THVNEGAADGQLAGLLGALDGALAAIESNLAAAWRETVVVVITEFGRTARINGSNGTD
                     HGTGTVALLVGGALKGGRVLADWPGLKAANL"
     gene            complement(1100..2542)
                     /locus_tag="VFR00_05745"
     CDS             complement(1100..2542)
                     /locus_tag="VFR00_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008132757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 family protein"
                     /protein_id="HEU0158791.1"
                     /translation="MATSSKALAALALNRFGLGPRLGSIAAIAADPRAAVLADLERPR
                     AGELAAPGLPSSAQAFRTVADANAERRARQITMAKAEEAKGQTGQTEPGMREGGEPTA
                     NAAAAKMAAEAVPNPGRQLYLNEAKARIDAALGSEIGFAERLVWFWSNHFCISADKIQ
                     SMSGAYEREAVRPHILGRFQDMLEAVESHPAMLFYLDNTVSIGPNSVAGINRTRGLNE
                     NLAREILELHTLGVRSVYTQEDIVHFANVLTGWTLLPAADNPDHGGEFVFNRRLHEPG
                     GQNILGKVYADAGIEQGKAVLHDLARHAATAKHVAGKLVRHFIADDPPPALIDGLAQT
                     FQDSDGDLKEVAKALVASEAAWSLPPTKLRRPSDWVTAMVRAGGGTTADPRRFTAGQA
                     TLGEPLWRPPSPKGYADDEASWIDGMGQRLDIAQTYAERVAGGLDPRFVIDSVLEGTV
                     SEETRQAVVRAESRQQALALLFMSPEFQRR"
BASE COUNT          408 a          855 c          884 g          399 t
ORIGIN      
        1 gcaggttcgc agccttaagc ccgggccaat cggcgagcac acgcccgcct ttgagcgccc
       61 cgccgaccag gagggcaacg gtgccggtgc cgtgatcggt gccgttgctg ccattgatgc
      121 gggcggttcg gccaaattcc gtgatcacga cgaccacggt ctcccgccag gccgccgcca
      181 agttgctttc gatggcggcc aaggctccgt cgagcgcgcc taacaggccg gcgagctgac
      241 cgtcggcggc gccttcgttg acatgggtgt cccaaccgac aaagcccatc gcgccaacgc
      301 gcggtccgtc ggggcgtgcg agatagcgtg cagcggtgcc ggcggcctcg gcaaagtagg
      361 cgcgcgcccg ggctaaaccc gccggcggca gctggggcga tgggccggcc gccgtgctgc
      421 cgtcggcgtg cgcgagcgcg gccaggcgga tgcgcccttc cagtgcggca gcgagcttga
      481 gatcggtgtg ctggtagagc tccagcaggc gtgcccgcgt gtcatcgctg gccggcacga
      541 ccagttgcgg ggcccacgac atcaccggcg cccgcccacg cacgaccagc ggggtgaccg
      601 ggcccaccgc cagggcccgc gcccccacgg gatcgacagg gccgccgggg gtcagcgcca
      661 gcagggcgcg attgagccaa ccgctgtcat agacgcccgg cttggcgaag ccgctctcca
      721 gcacgtcttg gccatcgaaa tgcgagcgct cccggtaggg ggtggcggtg gcgtgcacga
      781 tggtggccgc attggcttgg tagagccgat agagattggg catcgccggg ttgagggcaa
      841 aaaacgaatt gagcgcaagc gccggcgtct tgccgtcgag gaccaaagcg cgatcgccgc
      901 gcaagccgat ccaatcggga tcaccgaccg gcgccaccgc tcccaggcca tcgagcgccc
      961 cgcgcaagac gatcaccaac agccgcggat cgcggccttc cgcccgcgcc agtttgggga
     1021 gttgcgtcca ggcaaacagg gcgcccgaac ccagcaacgc ggcgcggcgg ctcggccaat
     1081 gctgacaggg agccatgggt cacctcctct ggaattcggg agacatgaac agcaatgcca
     1141 gcgcctgttg ccggctttcg gcgcgcacga ccgcctgcct ggtctcttct gagacggtgc
     1201 cttcgagcac gctgtcgatg acaaagcgcg gatcgagccc gcccgccaca cgctccgcgt
     1261 aggtctgggc gatgtcgagg cgctgtccca taccgtcgat ccagctcgcc tcatcgtcgg
     1321 catacccctt gggcgagggc ggacgccaca gcggttcgcc cagggtcgcc tgtcccgcgg
     1381 taaagcggcg agggtcggcc gtggtgccac cgcccgcgcg caccatggcg gtgacccaat
     1441 cgcttggccg gcggagcttg gtcggcggca aactccacgc tgcctcggac gccaccaggg
     1501 ccttggcgac ctccttgaga tcaccgtcgc tatcctggaa cgtctgcgcc aggccgtcga
     1561 tgagcgccgg cggcggatcg tcggcaatga agtgacgcac cagcttgccg gcaacgtgct
     1621 tggcggtggc ggcatggcgc gcgagatcgt gcagcacggc ttttccctgc tcgataccgg
     1681 cgtccgcata gaccttgccg agaatgtttt gccccccggg ttcgtgcagg cgtcgattaa
     1741 agacgaactc gccgccgtga tcgggattgt cggccgccgg cagcagtgtc cagcccgtca
     1801 gaacgttggc gaagtggaca atatcctcct gcgtatagac gctgcgcaca ccgagggtgt
     1861 gcagctcgag gatttcgcga gccaggttct cgttgaggcc gcgcgtacga ttgatgccgg
     1921 ccacggagtt cggaccgatc gagaccgtgt tgtccagata gaacagcatc gccggatggc
     1981 tttcgacggc ctccagcatg tcctgaaagc gaccgaggat gtgcggacgc acggcctcgc
     2041 gttcataggc acccgacatg ctttggattt tatcggccga gatgcagaaa tgattggacc
     2101 agaaccagac cagacgctcg gcaaaaccga tttcgctgcc gagcgcggcg tcgatgcggg
     2161 ctttggcctc gttcaaataa agttgccgac cgggattggg gacggcctcg gccgccattt
     2221 tcgcggccgc cgcgttggcg gtgggctccc caccctcgcg catcccaggc tcggtctggc
     2281 cggtctggcc cttggcctcc tccgccttgg ccatggtgat ctgtcgggcg cgccgttcgg
     2341 cattggcatc ggcgaccgtg cgaaaggcct gcgcgctcga gggcaagccc ggcgccgcca
     2401 gttcgccggc gcgcggtcgc tccaggtcgg ccagcacggc agcgcgtgga tcggcggcga
     2461 tggcggcaat ggatccgagg cgtggcccca ggccgaagcg gttgagcgcg agcgcggcga
     2521 gtgctttgct ggacgtagcc acggcg
//
LOCUS       DASTMT010000245         7514 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_3084136, whole genome shotgun sequence.
ACCESSION   DASTMT010000245 DASTMT010000000
VERSION     DASTMT010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 7514)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7514)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7514
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3084136"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1575
                     /locus_tag="VFR00_05750"
     CDS             <1..1575
                     /locus_tag="VFR00_05750"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU0158792.1"
                     /translation="AGYDGVEIMGSEGYLINEFTAPRCNDRNDQWGGALENRLRFAIA
                     VMRRVRQRTGPDFLVIFRVSSIDLVEGGLTGEEIGALARGVVAAGANIINQGVGWHEA
                     RVPTIGLRVPRAAWSFAARHLKESVAVPVVASNRINTPEVAEAILARGDADLVSMARP
                     FLADPEFANKTRQGRRDRINVCIACNQACLDLIFSQRPATCLVNPKAGRELDYDRPAP
                     ARRCRIAVVGAGPAGLACAVTAAERGHEVTLFEAAGRIGGQLNLARNVPGKEEFDETL
                     RYFHNRIADHGISLRLNCRPDAAMLEAAAFDEIVIATGARPRVPEIPGIAHGKCVSYV
                     ELLAGDKTAGARVAILGAGGIGFDVAEYLSSPSHAVGTDGERFLAEWGIDREIASPGG
                     LRETGDQSPTRQIVMLQRKPGRMGRSLGLSTGWALRLALAKRKVAQLSGVSYARIDDA
                     GLHILVEGAPRVLAADTIVICAGQEPVRDLYHELIARARRAHLIGGAERAAELDAMRA
                     IEQGTRLAYQFGATGA"
     gene            1678..2487
                     /locus_tag="VFR00_05755"
     CDS             1678..2487
                     /locus_tag="VFR00_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009755953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEU0158793.1"
                     /translation="MQDRYSHYKALKFARPRPRLLEIILSKPGRLNALDADGHAELAE
                     VWRDVDRDPDTSVAVLRGDGGVFSAGGDLEMVEVMARDWARRTRVWKEASDLVYNVIN
                     CSKPIVAAIEGPCVGAGLAAALLADISVAGRHARIIDGHTRLGVAAGDHSAIVWPLLC
                     GMAKAKYYLLLCDTLSGAEAERIGLVSMVADDDKVVEVALTAAERLRDMPPAAVRWTK
                     HALNNWLRLAGPTFDASLAMEFMGFSGPEVQEGLAALREKRRPKFDPTDPF"
     gene            complement(2515..2907)
                     /gene="secG"
                     /locus_tag="VFR00_05760"
     CDS             complement(2515..2907)
                     /gene="secG"
                     /locus_tag="VFR00_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEU0158794.1"
                     /translation="MTTVLLIIHLMIAAALVGVVLLQRSEGGALGIGGGGGFMTGRGA
                     ANFLTRVTASLAAAFFATSLLLTLLASRSTAPGSIFDSSGSPPAAGQAAPAKQPSPSE
                     PAAPAGRGGILDQLQPAAPGGPSVPQSK"
     gene            complement(2986..3567)
                     /locus_tag="VFR00_05765"
     CDS             complement(2986..3567)
                     /locus_tag="VFR00_05765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0158795.1"
                     /translation="MTRFGLVLGVQAWLLGAGAAAAATLDQEACARLKTELLQLELAG
                     TRNNMGKGPDWAKGNLSADKLLQIKRLLDVEEQLLFRCQGKPLVVLPEGVEAEPPQPA
                     DRAKEGALPTKAGEAKAAKEPQPPGKAQAKQPASKGPAAKAPSAPAAGAEKAPSAAAA
                     GQPRPKPKAKAKVDDAYRPPPATTPVSPGAAKP"
     gene            complement(3641..4411)
                     /gene="tpiA"
                     /locus_tag="VFR00_05770"
     CDS             complement(3641..4411)
                     /gene="tpiA"
                     /locus_tag="VFR00_05770"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012335101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEU0158796.1"
                     /translation="MAHASPLRPLIAGNWKMNGSVAALAEALAVRDRLAEPDFAAGVD
                     VMLCPPAPLLPLLAGELARGRLQLGAQDCHAAPSGAHTGDLSAELLKDVGASAVIVGH
                     SERRAEHGESDQEVRRKVSAAHRAGLTAIVCLGETAGERRAGLTLDVIGRQLARSLPE
                     HVRAADTVIAYEPVWAIGSGLSAKPEDVAEVHGFLRQALTQRCGEEGARLRLLYGGSV
                     KPDNAEQLLAIADVNGALVGGASLKAADFLGIVAACGA"
     gene            4593..6506
                     /locus_tag="VFR00_05775"
     CDS             4593..6506
                     /locus_tag="VFR00_05775"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HEU0158797.1"
                     /translation="MLDSLRRGALNWFAKGLLGLLVIAFAVWGIGDVIRNVGRGTLAT
                     IGSSHITVDEYRQAYQDEMASLTRRFGRRLTSEQAKLLGVEQRALARLISAAAIDHHA
                     RDLKLALSDQGIADIIRNDPGFQGPDGKFSKELFQNFLRQIGLSEGRYVYTRRQEELR
                     DQLTDTLLSGLAAPAAMIDALYRYREERRSIDFFTPDFDKLIKLGEPDDAKLRAFYEE
                     NKARFTAPERRKITVLLLTREAAKQRIAVSEDDLKAAYDADKQKFNIPEMRRITQLTF
                     PDKAAAEKAYAELHKAENFAEAVNKLGFKESDIELGLLAKRDMIDSRIAEAAFGLNKD
                     ELSQPVEGQFATVLLRVTEIVPGKQRSFEEVKAEIGDRLAEERANLELQNLHESVEND
                     RYAGKSLKEIAEKLKLPFLEIADIDRTGKTAEGKPAIEHADAGKIAQAAFTGNAGIEA
                     EAVELADGGNAWVDVLQVTPEKQKTFEEVAAEVKTQYLEQQRRTEVGNLAAKFVDRLN
                     QGEAFETVASEAGAKPEKTGPVTRSTTPQGLSNNAVQLAFTLAKGHAASSPTTDGKAR
                     TIMRVDDITPAPAPTPEQLSTLRGELNRQMQSDILAEYIAGLQARYGLSVNQEALRQA
                     LGGERGGEAPSIE"
     gene            6540..>7514
                     /locus_tag="VFR00_05780"
     CDS             6540..>7514
                     /locus_tag="VFR00_05780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013418060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HEU0158798.1"
                     /translation="MTKLEPVGRDLEVDPPRGEFARRYAAGQPQVVWTRLIADLETPV
                     SAMLKLSDGQPLSCLLESVQGGATRGRYSIIGLAPDLVWRAFANRAEVNERPLAAPAA
                     FRPETAPTLQSLRALLAASRIELPAELPPIAAGIIGYMGYDTVRLIEHLPHEKPDTLG
                     VPDAVFVRPTVMVVFDTIKDEMAVVTPVRPAPGIDAGAAYAAAIGRLKGVLAALDAPL
                     PHATNMSGIDLALPDAVSNTSPAEFKAMVQRGKEYIAAGDVFQVVLSQRFDAPFALPP
                     LALYRALRRTNPSPFLFHLDFGGFALVGSSPEILVRVRDGEVTIRPLAG"
BASE COUNT         1245 a         2593 c         2503 g         1173 t
ORIGIN      
        1 gccggctacg acggcgtcga gatcatgggt tcggaaggct atctgatcaa cgagtttacg
       61 gcgccgcgct gcaacgatcg taacgatcaa tggggcggcg cgctcgagaa ccggctgaga
      121 tttgccattg ccgtcatgcg ccgcgtgcgc cagcgcacgg gccccgattt tctggtgatt
      181 ttccgtgtct cctcgatcga cctggtcgag ggcggactga cgggcgagga aatcggcgcc
      241 ttggcgcgcg gcgtggtcgc cgcgggcgcc aacatcatca accaaggcgt cggctggcac
      301 gaagcgcgcg tgccgaccat cggcctcaga gtgccgcgcg cggcctggag cttcgcggcg
      361 cggcatctga aagagagcgt ggcggtcccg gtggtggcct ccaaccgcat caataccccc
      421 gaggtcgcag aagcgatcct agcccgcggg gatgccgatc tcgtctccat ggcccggccg
      481 ttcctggccg accccgagtt cgccaataag acgcgccagg gccgccgcga tcgcattaat
      541 gtgtgcatcg cctgcaatca ggcctgcctc gatctgatct tttcgcaacg gccagcgacg
      601 tgtctcgtca atcccaaggc cggacgcgag ctcgactacg atcggcccgc gccggcacgc
      661 cgctgccgca tcgccgtggt gggcgccggc ccggccggtc tggcctgtgc ggtgacggcc
      721 gccgagcgcg gccacgaggt gacgctgttt gaagccgccg ggcgcatcgg cggacagctg
      781 aatttggcgc gcaacgtgcc tggcaaggag gagttcgacg aaacgctgcg ctactttcac
      841 aaccgcatcg ccgaccacgg catcagcctg cgcctcaact gccgtccgga cgcggccatg
      901 ctggaggccg ccgcgttcga cgagatcgtc atcgccacgg gagcccggcc ccgggttccc
      961 gagatcccgg gcatcgcgca cggcaaatgc gtgagctacg tcgagctatt ggcgggcgac
     1021 aaaaccgcgg gcgcacgcgt cgccatcctg ggcgccggcg gcatcggctt cgatgtcgcc
     1081 gaatacctga gcagccccag ccatgccgtt ggcacggacg gcgagcgttt ccttgccgaa
     1141 tggggaatag atcgcgaaat tgcgagccct ggtggcctgc gcgagaccgg cgaccagagt
     1201 ccgacgcggc agatcgtcat gctgcagaga aagcctgggc gcatgggacg cagcctcggt
     1261 ctcagcaccg gctgggccct gcgcctggcg ctcgccaaac gcaaggtggc gcagctgagc
     1321 ggcgttagct acgcacgtat cgacgatgcc ggcttgcaca tccttgtcga gggggccccg
     1381 cgcgtgctcg ccgccgacac catcgtcatt tgcgccgggc aggagccggt gcgcgatctc
     1441 taccacgagc tcatcgcccg cgctcgccgc gcgcatctga tcggtggtgc cgaacgggcc
     1501 gccgagctcg atgccatgcg cgcgatcgag caaggcacgc ggctcgccta ccagtttggt
     1561 gccaccggcg cctgagcccg ccgtctgcgg cgccagggcc ccgcgccctg actggcccga
     1621 ggcggcgccg aagcgaggcc gcggcgatca cactcgccaa gcaaaaggag gtgcaacatg
     1681 caagatcgct acagccacta caaggccctc aagtttgcaa ggccgcggcc gcgcctgttg
     1741 gagatcattc tctcaaaacc gggcagattg aacgcgctcg atgccgacgg gcatgccgaa
     1801 ttggccgagg tttggcgcga tgtcgaccgc gatcccgaca cctccgtcgc cgtactgcgc
     1861 ggcgacggcg gcgttttctc ggccggtggc gacctcgaga tggtcgaagt gatggcccgc
     1921 gattgggcga gacgcacccg cgtctggaag gaggcgagcg acctcgtcta caacgtcatc
     1981 aactgcagca agcccatcgt tgccgccatc gaggggccct gcgtcggtgc cggcctggca
     2041 gcggccctgc tggccgacat ctccgtggcc ggtcgccatg cgcgcatcat cgatggccac
     2101 acccggctcg gcgtggccgc cggcgatcat tccgccatcg tctggccgtt gctgtgcggc
     2161 atggccaaag ccaaatacta cctgttgttg tgcgacaccc tctccggcgc ggaagccgag
     2221 cgcatcggtc tcgtctccat ggttgccgac gacgacaagg tggtggaggt ggcgctgacg
     2281 gcggccgagc gtttgcgcga catgccgccg gcggcggtgc gctggaccaa gcacgccctc
     2341 aacaactggc tgcgtctggc ggggccgacc ttcgatgcct ccctcgccat ggagttcatg
     2401 ggcttctcgg gacccgaggt gcaggaaggc ttagccgcgc tgcgcgagaa gcggcggcct
     2461 aagttcgatc caacggatcc cttttgatcg cgcgggccca aaagcccaag cgcgctactt
     2521 cgattgcggc acgctcggac caccaggcgc ggcgggctgc aactggtcga gaatgccgcc
     2581 gcgccccgcc ggggccgccg gctcgcttgg cgagggctgc ttggccggcg ccgcttggcc
     2641 cgctgccggt ggcgagcccg aactatcgaa gatcgagccc ggcgccgtcg accgcgatgc
     2701 cagcagggtc aacaacaggc tggtggcaaa aaaggcggcc gccaggctcg ccgtcacgcg
     2761 ggtgagaaaa ttcgccgcgc cgcggccggt catgaagccg ccaccgccgc cgatcccgag
     2821 cgcaccgcct tcggagcgct gcaacagcac gacgcccacc aaggcggcgg caatcatcaa
     2881 atgaataatg aggaggacgg tcgtcatgcg atcatcttcg ttgctgggag ctcgagggct
     2941 tctacaccac gcgcgccaac cccaccagcc ctcaacgggc gcccgttaag gcttggcggc
     3001 gccggggctg acaggggtgg ttgccggcgg cggccggtag gcgtcgtcga ctttggcttt
     3061 tgctttcggt ttgggcctcg gctggccggc cgctgcggcg gagggcgcct tctccgcccc
     3121 ggccgccggc gcgctgggcg ctttggccgc cggccccttg ctggcgggct gcttggcttg
     3181 ggctttgcct ggcggctggg gctccttggc cgccttcgcc tcacccgcct tggttggcag
     3241 cgcgccctct ttggcgcggt cggcgggctg cggcggctcc gcctccacgc cctcgggcag
     3301 cacgaccagc ggcttgccct ggcagcgaaa caaaagctgc tcctcgacgt cgagcagccg
     3361 tttgatctgc aagagcttgt cggcgctgag attgcccttc gcccagtccg ggcccttgcc
     3421 catgttgttg cgcgtgccgg cgagctcgag ttgcaggagc tcggtcttga ggcgggcgca
     3481 ggcctcctga tcgagggtcg cggccgcggc cgcaccggcg cccagcagcc aagcctgcac
     3541 gccaagcacc aggccgaaac gggtcatgtg cgtttgaaca cggtcatgcg caagggagac
     3601 aagattgggg ggcgactaag gcctcattcg cgccgcccgg tcaagcgccg caagccgcaa
     3661 cgatgccgag aaaatcggcg gccttgaggc tggcgccgcc caccagggcg ccgttgacgt
     3721 cggcgattgc cagcaattgt tcggcattgt ccggcttgac ggagccgcca tagagcagac
     3781 gcaggcgcgc tccctcttcg ccgcagcgct gggtgagcgc ctggcgcaga aagccgtgca
     3841 cctcggcgac atcctccggt ttggcgctga gcccactgcc gatggcccac acgggctcgt
     3901 aggcgatcac ggtatcggcg gcccgcacgt gctcgggcaa ggaacgggca agctgcctgc
     3961 cgatcacgtc gagcgtcagg ccggcgcggc gttcgcccgc ggtttcgccc agacagacga
     4021 tggcggtcag gccggcacga tgcgcggcgc tgaccttgcg ccgaacctcc tgatcgcttt
     4081 cgccgtgctc ggcgcgccgt tccgaatggc ccacgatcac ggcgctggcg cccacatcct
     4141 tgaggagctc ggcggaaagg tcgccggtgt gcgccccgct cggcgcggcg tggcaatcct
     4201 gggcgccaag ctgcagccgt ccccgcgcca gttcccccgc cagcaagggc aagagcggcg
     4261 cggggggaca aagcatcaca tcgaccccgg cggcgaagtc cggctcggcc agccggtcgc
     4321 gcacggccag cgcctcggca agcgctgcca cggagccgtt cattttccag ttgccggcaa
     4381 tcagcggccg cagcggggac gcgtgtgcca tctctcttct cctcgctcgc attaagtttc
     4441 ggcaatggtc ggccgccgag ccagcggagc accttgctgg cgcccgccgg cgttcataat
     4501 atgccgcgca cgcgcgtcaa gatgtgctaa gcccctcacc ggtggggccg gttccctggc
     4561 catcggcgct ccctgcaact ggaaatgcta ccatgctcga ctccttgcgc cgcggcgcgc
     4621 tcaattggtt cgcgaagggg ctgcttgggc tgctggtcat cgccttcgcg gtctggggca
     4681 tcggcgacgt catccgcaac gtcggcaggg gcacgcttgc caccatcggc agctcgcaca
     4741 ttaccgtcga tgagtaccgt caggcctacc aggacgaaat ggcctccctt acgcgccgct
     4801 tcggccgccg cctcaccagc gaacaggcca agctgctcgg ggtggagcag cgcgcgctgg
     4861 cgcgcctgat ttccgcggcc gccatcgacc atcacgcgcg cgacctcaag ctggccctgt
     4921 ccgaccaggg gatcgccgac atcattcgca acgatccagg tttccagggt cccgacggca
     4981 agttttccaa ggagctgttc cagaacttct tgcgccagat cggcttgagc gaggggcgct
     5041 acgtctacac ccgccggcag gaggagctgc gcgatcagct gaccgacacc ttgttgagcg
     5101 gcttggcggc gccggcggcc atgatcgatg ccctttatcg ctaccgcgag gagcggcgca
     5161 gcatcgattt cttcacgccc gacttcgaca agctcatcaa gctcggggag ccggacgatg
     5221 ccaagctgcg cgccttctac gaggagaaca aggcgcgctt cacggcgccc gagcggcgca
     5281 agatcacggt cttgctgttg acgcgcgagg cggccaagca gcgcatcgcc gtcagcgagg
     5341 acgacctcaa ggccgcttac gacgccgaca agcagaagtt caatatcccg gagatgcggc
     5401 gcatcacgca gctcaccttc cccgacaagg cggcggccga aaaggcctat gccgagctcc
     5461 acaaggccga gaatttcgcc gaggccgtca acaagctggg cttcaaggaa agcgatatcg
     5521 agctgggcct cctcgccaaa cgcgacatga tcgacagccg cattgcggaa gcggccttcg
     5581 ggctcaacaa ggacgagctg tcacaacccg tcgagggtca gtttgccacg gtattgctgc
     5641 gcgtcaccga gatcgtaccg ggcaagcagc gcagtttcga ggaggtgaag gccgagatcg
     5701 gcgatcgtct cgccgaagag cgggccaatc tcgagctgca gaacctgcac gagagcgtcg
     5761 agaatgaccg ttacgcgggc aagtcgctga aggagatcgc cgaaaaactc aagctgccgt
     5821 tcctggagat cgccgacatc gaccggacgg gtaagaccgc cgagggtaag ccggcgatcg
     5881 agcacgccga tgccggcaaa attgcccagg cggcctttac cggcaatgcc ggcatcgagg
     5941 cagaggccgt ggaactggcc gatggcggca acgcctgggt ggatgtgctg caagtcacgc
     6001 cggagaagca aaagaccttc gaggaggtgg ccgcggaggt gaagacccaa tatctggagc
     6061 agcagcgccg aaccgaggtc ggcaatctcg cggcgaaatt cgtcgatcgc ctcaaccagg
     6121 gggaggcgtt cgagaccgtg gccagcgaag ccggcgccaa gccggagaag accggccccg
     6181 tcacgcgctc gaccacaccg cagggcctca gcaacaacgc cgtgcaactg gccttcacgc
     6241 tggcgaaggg ccatgccgcc tcctccccca cgacggacgg caaggcgcgc acgatcatgc
     6301 gcgtcgatga catcaccccg gcgcccgcgc ccacccccga gcagctcagc acgcttagag
     6361 gagagctcaa ccggcaaatg cagagcgaca tccttgccga atacatcgcc ggcctgcagg
     6421 cccgctacgg cctgtcggtc aatcaggagg cgctgcgaca ggcgctcggc ggggagcgcg
     6481 gtggcgaggc acccagcatc gaataacgct tacagaccgg gcccaaggat ccgcgcccca
     6541 tgaccaagct cgagccggtc ggcagagacc tcgaagttga tcccccgcgc ggcgaattcg
     6601 cgcgccgcta cgccgccggg cagccgcagg tcgtgtggac gcgattgatc gccgatctcg
     6661 aaaccccggt ctccgcgatg ctgaagctga gtgacggcca gccgctcagc tgcctgttgg
     6721 aatcggtgca aggcggcgcc acgcgcggtc gctactccat catcgggctg gcgcccgatc
     6781 tggtctggcg cgcctttgcc aaccgcgccg aggtgaatga gcgcccgctg gcggcgccgg
     6841 cggccttcag gccggagacc gccccgacgc tgcagtcgtt gcgcgcgctg cttgccgcct
     6901 cccgcatcga gctgccggcc gagctgccgc cgatcgcggc cggtatcatc ggctacatgg
     6961 gctacgatac ggtgcgcttg atcgagcacc tgccgcacga gaagcccgac accttgggcg
     7021 tacccgacgc cgtttttgtt cgccccaccg tgatggtggt gttcgacacc atcaaggacg
     7081 agatggcggt ggtgacgccc gtgcgtcccg ccccgggcat cgatgccggg gccgcctatg
     7141 ccgccgccat cggacgcttg aaaggtgtgc tcgccgcgct cgatgcgccg ttgccccatg
     7201 ccaccaacat gtcggggatc gacctggcgc tgcccgatgc ggtctccaac acgtcgcccg
     7261 ccgaattcaa ggccatggtg caacgtggca aagagtacat cgccgccggg gacgtcttcc
     7321 aggtcgtgct ctcccagcgc ttcgatgcgc ctttcgccct gccgcccctg gcgctttatc
     7381 gcgcgctgcg gcgcacgaac ccctccccgt tcctgtttca cttggacttc ggcggcttcg
     7441 ccctcgtcgg ctcctcgccg gaaatcctgg tgcgcgtgcg cgacggcgag gtcaccatcc
     7501 ggccgctggc cggc
//
LOCUS       DASTMT010000246         2160 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_460152, whole genome shotgun sequence.
ACCESSION   DASTMT010000246 DASTMT010000000
VERSION     DASTMT010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2160
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_460152"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..2160
                     /locus_tag="VFR00_05785"
                     /pseudo
     CDS             1..2160
                     /locus_tag="VFR00_05785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015319318.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
BASE COUNT          372 a          751 c          662 g          375 t
ORIGIN      
        1 tgacgctcgc ctctggctgg ctcggcgagg tgcgccgcaa ggatccaagc cggctcgcct
       61 ttttcaccgg acgcgatcag agccaggccc tgaccggctg gtgggccatg cagtttggca
      121 ctccgaattt cgccgcccac ggcggtttct gttcggtcaa catggcggcg gcgggtcttt
      181 aaaccttcgg tggctcgttc tgggaattcg gtgagaccga ttgggaccac acccgctatt
      241 tcatgctgtg gggcgtggcg gaggaccacg cctccaatcc cctcaagatc gagcttggca
      301 agttgaaggc gcgcggcgtc aaggtggtga gcgtcaatcc cgtgcgcacc ggctacggcg
      361 ccatagccga cgaatggatc ggcattaagc cggggacgga tggcctgttc gcaggcgccc
      421 tggtgcatga gcttctgacc aaacaccaga tcgacgtcga ctacttgcgc cgttacacta
      481 atgccggctg gctggtgatt gcggccccgg gcgcggccga cgacgggctg atcgcgcgcg
      541 acgctgaggg gagaccgctc gtctacgaca aacgcaccgg tcggctcgcg agcgcgctcg
      601 accccgatgt cacccccgcc ttttcgggta ccttccggct cgccgatgcg cgtgaggcgg
      661 tgccggtatt ccagcttttg gcgcagcgtt tcttgtcgcc ggactacgca cccgaacgcg
      721 tcgccggcgc caccggcatt gccgccggca cgatccgccg cattgccagc gagctggcgc
      781 aggtggcctt tgcccagccg gtggtgctcg ataccccctg gaccgactgg accggccggc
      841 gccacgataa gacgattggc aggccggtgg gaatgcatgc catgcgcggc atctcggccc
      901 acgccaacgg ctttcacacc tgccgcatcc tgcacgtgct gcaaatcctg ctgggctcga
      961 tcgattgccc gggcggcttc cgctacaaga cgccttatcc aaagcagacg ccgccgacgc
     1021 tcaaacccaa aggcaagccg ggcgaggtca agccgatgca ggccttgcag ggaccccatc
     1081 tcggcttccc gaccgggcct gaggatctgc tggtggatca ggcggggagc ccgcaacgca
     1141 tcgataaggc ttacagctgg gaggcaccgt tcgccgcgca cgggctgatg cacacggtga
     1201 tcgccaatgc tgcccgtggt gatccctacc ctatcgacgt gctgttcctg tacatggcca
     1261 acatgggctg gaactcggcg atgaacacgc cggcgaccct ggcccaccta agcgcgaagg
     1321 acccggctac gggcgcctac aagattgcga agatcatcta ctcggacgcc tacttctccg
     1381 agacggttcc ctacgccgac ctggtactgc cggacacgac atatctggag cgctgggact
     1441 gtatctcgct gctcgaccgg cccatttccg aacccaacgc ccccgccgat gccatccgcc
     1501 agccggtagt cgctcccgat cgcgacgtgc ggccgttcca aagcgtgctg atcgatcttg
     1561 gggcgcgcct ccgccttccg ggcttcagca atgccgacgg ctcgccgaaa taccctggcg
     1621 gctacgccga ctacattgtc aatcacgagc gccggccggg catcggcccg ctggcgggac
     1681 atcgcggcaa gaccggctcc gactggggtc gcggcgagcc caacccacgc cagctcgagc
     1741 aatatattgc cgatggctgc gtccacgtcc accacctgcc cgagccgttg cgctatttca
     1801 agcacgccaa ccgcgccttc atcgactggg ccgtcgacat gggctttcgc atcacgccga
     1861 cgccggtgat ctttcagctc tacagcgaac ccatgcaaaa gatgcgcctg gccgcgcgag
     1921 gacacggggc cgttctgccg ccgccccagc atcgacagcg catcgagacc tacttcgatc
     1981 cgctgccctt ctggtatgcg ccgtttgaag aagcccacac cggcgaccgc gactttccgc
     2041 tgtatgccat cacgcagcgg ccgatggcga tgtaccattc ctggggctcg caaaacgcct
     2101 ggctgcgcca gatccacacc gcgaaccggc tgtatttggg acgagcgcgc gccgccaagc
//
LOCUS       DASTMT010000247         2383 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_4068057, whole genome shotgun sequence.
ACCESSION   DASTMT010000247 DASTMT010000000
VERSION     DASTMT010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2383
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4068057"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..209)
                     /locus_tag="VFR00_05790"
     CDS             complement(<1..209)
                     /locus_tag="VFR00_05790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0158799.1"
                     /translation="MRIVALEEHFTVPSLVRRISPEAIARRGFGKRTPVPGRVNPLEL
                     LPEIGSTRLSSMDAAGISVQVLSTSG"
     gene            367..537
                     /locus_tag="VFR00_05795"
     CDS             367..537
                     /locus_tag="VFR00_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0158800.1"
                     /translation="MAVRSLRNVEKQKKKARRALPRKKLTRIQGNTLGARRQLKKMAG
                     KARAAKAAAAKS"
     gene            complement(677..2170)
                     /gene="gatB"
                     /locus_tag="VFR00_05800"
     CDS             complement(677..2170)
                     /gene="gatB"
                     /locus_tag="VFR00_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEU0158801.1"
                     /translation="MGQESKARAATDAGGRGDWEIVVGLEVHAQVASQAKLFSGAATE
                     FGAAPNTQVSLVDAAMPGMLPVINQGCIDQAVRTGLGLEAQINLNSVFDRKNYFYPDL
                     PQGYQISQYKQPIVGAGKVEIEVDGEIYSIGIERLHLEQDAGKLIHDQHPDYSLVDLN
                     RSGVALMEIVSKPDLRSAKQAQAYVSKLRSILRYLGTCDGDMEKGNLRADVNVSVRRK
                     GATEFGTRCEIKNVNSIRFIGLAIEAEARRQIEILEDGGSIQQETRLFDAGRGETRSM
                     RSKEEAHDYRYFPDPDLLPLELTPDYVEALKRGLPELPDAKVQRFMQDYKLSAYDARV
                     LTAERESADFFEAVAQGQMSGGPRDGKMVANWVINELFGRLNKEGLTISANPVSSAQL
                     GSIIDLIGSGAISGKIAKDVFEIVWTEGGDPAAIVAQKGLRQLTDTGAITTAIDAIIA
                     ANPDKVAAAKAKPAMLGWFVGQVMKATGGKANPQAVNALLKEKLNLA"
BASE COUNT          395 a          759 c          777 g          452 t
ORIGIN      
        1 cctgacgtcg atagcacctg gacgctgatg ccggccgcat ccatggagct gagccgcgtg
       61 ctgccgatct cgggcaaaag ctcgagcgga ttgacgcggc ccggaaccgg ggtacgtttg
      121 ccgaacccgc gtctggcgat agcctcggga ctgatccggc gcaccagcga cggcaccgtg
      181 aaatgctcct cgagcgcaac gattcgcatg ggggccatct ccttgtttcc tcgcgcctca
      241 cgttgtagcc aggctgtggc ggcgcgcaaa ggccgctcgc gtcgccgtgt gggcagattt
      301 gaacttgtgc ggccaaggga aagaccttac atcggcggcg aaaagcgagc gagacaggag
      361 ctcagcatgg cggttcgttc actgcgcaac gtcgagaagc agaagaaaaa ggcgcgccgg
      421 gcattgccgc gcaagaagct cacgcgcatc cagggcaaca ccttgggcgc ccgccgccag
      481 ctgaagaaga tggcgggcaa ggcccgcgcc gccaaggcgg cggcggccaa gagctgaacg
      541 gtttttctcg ctcggccgcg gctgcctccc cggccacgcg ccgccggcct taggctcagc
      601 gtggctgtgg catttaggtg gctgggcgcg gtgcgccggc gcgctaaccc gccgccgggg
      661 ctcttcgcca cggccttcac gccaggttca gcttttcctt gagcagcgcg ttgaccgcct
      721 gcggattggc cttgccgccg gtggccttca tgacctggcc gacgaaccag ccgagcatgg
      781 cgggtttggc cttggcagcg gccaccttgt cgggattggc ggcaatgatg gcgtcgatgg
      841 ccgtcgtgat ggcgcccgtg tcggtcagct gcctgagccc cttctgtgcg acgatcgctg
      901 cggggtcgcc gccctcggtc cagacaattt cgaacacgtc cttggcgatc ttaccggaga
      961 tcgcacccga gccgatcaga tcgatgatac tgccgagctg agcgctggag accgggttcg
     1021 cgctgatcgt caggccctcc ttgttcaagc gcccgaatag ctcgttgatc acccagttgg
     1081 ccaccatctt gccatcgcgt ggcccaccgc tcatttgtcc ctgggccacg gcctcgaaga
     1141 aatcggcgct ctcgcgctcg gccgtgagga cgcgcgcgtc gtaggcggac agcttgtaat
     1201 cctgcatgaa gcgctgaacc ttggcgtccg gcagttcggg taagcctctt ttcaaagcct
     1261 cgacgtaatc tggcgtcaat tcgagcggca acaagtcggg gtcggggaag tagcgatagt
     1321 cgtgggcttc ctccttggag cgcatcgagc gggtctcgcc tctgcccgca tcgaagagcc
     1381 gcgtctcctg ctggatgctg ccgccatctt cgaggatctc gatttggcgc cgcgcctcgg
     1441 cctcgatcgc cagaccgata aagcgaatgg aattgacgtt cttgatttcg caacgcgtgc
     1501 cgaactcggt tgcgcccttg cgccgcacgg agacgttgac gtcggcgcgc aagttgcctt
     1561 tttccatgtc gccgtcgcag gtgcctagat agcgcaggat cgaacgcagc ttggaaacgt
     1621 aggcctgcgc ctgtttggcc gaacgcaggt cgggtttgga gacgatctcc atgagcgcta
     1681 cccccgagcg attgagatcg accagggaat agtcgggatg ctgatcgtga atgagtttgc
     1741 ctgcatcctg ctccaggtgc agccgctcga tgccaatgct gtaaatctcc ccatcgacct
     1801 cgatttcgac cttgccggcg ccgacaatgg gctgcttgta ctgggagatc tgatagccct
     1861 gcggcaaatc ggggtagaag taatttttcc ggtcgaacac ggagttgaga ttgatctgcg
     1921 cctcaagccc gaggcccgtg cgcacggcct gatcgatgca gccctgattg atgaccggca
     1981 gcatgccggg catggcggca tcgaccaggg agacctgggt attgggcgcg gcaccgaact
     2041 cggtggcggc gcccgagaat aacttggcct gcgaggcgac ttgggcatgg acctcgaggc
     2101 cgacgacgat ttcccagtcg cccctgccgc cggcatcggt ggcagcgcgc gctttgcttt
     2161 cctgacccat gctgagctcg gcgcctcctc agcgctcgca atggtgcagc cttgctcggg
     2221 ccgagcccgg ctaccgccga gcgcgccccg ccggcggcgg cccgccgccg cgaccttgcc
     2281 tagagggcag aggacgggtg ggtcaagcgc cttgcgccca tttgccaatt tggggcccgg
     2341 ccctacagtc ggtccccttt tggatgttga ggagcactgc cat
//
LOCUS       DASTMT010000248         5194 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_1443019, whole genome shotgun sequence.
ACCESSION   DASTMT010000248 DASTMT010000000
VERSION     DASTMT010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 5194)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5194)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5194
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1443019"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(320..1288)
                     /locus_tag="VFR00_05805"
     CDS             complement(320..1288)
                     /locus_tag="VFR00_05805"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HEU0158802.1"
                     /translation="MPVVEAERLSKHFPGRGRLLGGTRGVVRAVDGISFSIEAGQTLG
                     VVGESGCGKTTTAKLVLGLEQPTAGDIRFEGQDLATLDTAGRRHYRRSVQAVFQDPYA
                     SLNPRMRVSAIIAEPLVTNERLTAAAVTKRVLELLELVGLPRRAADLFPHEFSGGQRQ
                     RIAIARALSLSPKLIVLDEPVSALDVSIRAQILNLLRDLQTQLNLSYLFIAHDLAAVA
                     HMSHAIVVMYLGRIVEYGATRALLGAPKHPYTKALFSAALPSRPDEAREDIVLAGEVP
                     SPLNPPPGCHFHPRCQQAMRQCGEKDPGLVALEDRHVACHLYGARA"
     gene            complement(1288..2298)
                     /locus_tag="VFR00_05810"
     CDS             complement(1288..2298)
                     /locus_tag="VFR00_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007756208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0158803.1"
                     /translation="MAAAPLLRIENLSTHYVSARGTRVVRAVDGVSLSIGARETLGIV
                     GESGSGKSTLALSVLRLLPPSARIAGGRMLFEGEDLLQKSEAEMRRLRGRRIAMILQD
                     PMASLNPLFTVGDQVAEPIRVHERAKRRTAWRRALDLLRAVRIASPATRLLQFPHEMS
                     GGMRQRIVGAIGISCEPRLLIADEPTTSLDLTIQAQYLNLLRELQQAHGLALIFITHN
                     LGIVAKMCDQLAVMYAGQLVEAGPVRQIFDSPAHPYTQALLNSIPRLSDNRRRLSAID
                     GQPPDLGLMPAGCAFAPRCSQAIDRCRSAPPPEVSIGDRRHAKCWLLAADASDKVKRG
                     AA"
     gene            complement(2330..3208)
                     /locus_tag="VFR00_05815"
     CDS             complement(2330..3208)
                     /locus_tag="VFR00_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009490190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0158804.1"
                     /translation="MAIETPAVELSPRFSFRLADFPLLPTLILTSIALVALFAGALAP
                     HDPEIGSLAARFRPPAWQAGGSLEHLLGTDHLGRDVLSRLIFGARVSVVVGFTAVVVA
                     GALGTLLGIISGYLGGWVDQVIMRITDTWLALPALTFAIFLTAIVGPSEMNIVIILAA
                     VYWTRYARVIRGEVLSLKEREFVRLAVVAGCSKWTIMRRHILPNVLNSAIVLGTLMLG
                     VVIVTEAALSFLGVGVPPPKPAWGLMLADGKKGMMVGYWWLTVLPGLCIMLMVLSANL
                     LGDWLRVKLDPQLRQL"
     gene            complement(3213..4133)
                     /locus_tag="VFR00_05820"
     CDS             complement(3213..4133)
                     /locus_tag="VFR00_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0158805.1"
                     /translation="MTHYILRRLGYSLVSLLLLSLTIFLFVRVTGDPTTLLVEPGAPQ
                     ADIDAIRQRFGLDQPIWVQYWTFLASLLTGDFGDSFYYRTPVLDLYLTRLPNSLLLAL
                     AAMAFSLIIGIPSGILAAVRVGGFWDSAGKTFSLLGLSLPSFWVGLVLILIFSVSLGW
                     LPSSGSGTVWHLLMPAFALGWYFAAAHMRLTRSSMLEVLGSEYIKLARLKGLPEPRVI
                     AKHAFKNALIPVLTLAGINLVIMINVAVVVETVFAWPGIGRLLYEGITFRDFPVVQAV
                     VLLGGAMIVFVNLLIDILYALIDPRIRLER"
     gene            complement(4268..>5194)
                     /locus_tag="VFR00_05825"
     CDS             complement(4268..>5194)
                     /locus_tag="VFR00_05825"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0158806.1"
                     /translation="SLEAFPEYWRKKPNVERLILRVIPDEATRLAALKRGEVDIAYSI
                     RGELAEELERSKGLSLKPTVIQAPFWLYFPDQWDPKSPWHDPRVRRAASLAIDHKGIN
                     QALTLGHSKITGSIIPYTFEQYWQPPLPSYDPTLAKKLLAEAGHPNGFDAGEYYCDVS
                     YANLAEAVLNNLQAIGIRAKLRPLERVAFFKGYAEKSFKNLIQGASGAFGNAATRLES
                     FVVTGGTYVYGSYPDIDALFSSQASELDAAKRGALLNKIQELVHERTIYAHLWQLAFL
                     NGAGPRVGESGLGLIAGHAYSAPYEDVTVVTK"
BASE COUNT          987 a         1583 c         1819 g          805 t
ORIGIN      
        1 tcttccgcgc gatccaatcg tgaacgcggt cgaggtcgat gcgggcgagc tcgcacgtgt
       61 aggcgatgct gtcttggaga ttggcggggg gtgcggcgag aaacatgggc ggagcacctc
      121 ggcttggcag cgccaaaaaa cgaaaaagcc gcgcaacggg cgcggcttgc ggacgaacgc
      181 tactcgaacg tcatcgcttg cgcgccaggg gcggccaggc tggacgtcgg cgtcgctgtc
      241 tcgaagcaag gtgcatggtg ggcgtggtgg taagggcgat ccggctcgaa gtcaacggcg
      301 tcggcagcca agccggtcgt caggcccgcg ccccgtaaag gtggcaggcc acgtggcgat
      361 cctcgagggc aacgaggccg gggtcttttt cgccacactg gcgcatcgcc tgctggcaac
      421 ggggatggaa atggcaaccg ggcggcgggt tgagcgggct cggcacttcg ccggcgagca
      481 cgatgtcctc gcgcgcttcg tcgggccggg agggcagcgc cgcggaaaac agggccttgg
      541 tatagggatg cttcggggcc cccagcagcg cccgcgtcgc gccgtactcg acgatgcgac
      601 caagatacat gaccacgatc gcgtggctca tatgcgccac ggcggccaga tcatgggcaa
      661 tgaacaaata ggagagatta agctgcgttt gcaggtcgcg cagcagattg aggatttgcg
      721 cccggatcga gacgtcgagc gcggacaccg gctcgtccag aacgatcaac ttgggtgaca
      781 gcgataaggc ccgcgcgatg gcgatgcgct ggcgctgacc gccggagaat tcgtgtggga
      841 agaggtcggc ggcgcggcgg gggagcccga ccagctccaa cagttcgagc acccgcttcg
      901 tgactgcggc ggccgtcagg cgctcgttgg taacgagcgg ctcggcaatg atggcgctca
      961 cgcgcatgcg cgggttgagc gaagcgtaag gatcttgaaa cacggcctgc acggaccggc
     1021 gataatgccg gcgtccggcg gtatccagcg tggcgagatc ctgtccctcg aagcgaatgt
     1081 cgcccgccgt gggctgctcg aggccgagca ccagtttggc ggtggtggtt ttgccgcaac
     1141 cggactcgcc gaccacgccg agggtttggc ccgcttcgat gctaaaggag atcccatcga
     1201 cggcgcgaac cacgccccgt gtgccgccga gcaggcgacc gcggccggga aaatgcttgc
     1261 ttagccgttc ggcctcgacc acgggcatca ggccgctccg cgtttgacct tgtcactcgc
     1321 gtctgccgcg aggagccagc acttggcatg ccgtcgatcc ccgatcgaga cttccggcgg
     1381 gggcgcgctg cggcagcgat caatggcctg cgagcagcgc ggggcaaagg cacaccccgc
     1441 cggcatcagc ccgaggtcgg gcggctggcc gtcgatggcg ctgagccggc gtcggttgtc
     1501 gctcaggcgc ggaatggagt tgagaagcgc ctgggtgtag gggtgcgctg ggctgtcaaa
     1561 aatctgccga acggggcccg cctccaccag ctgtcccgca tacatgacgg caagctggtc
     1621 gcacatcttg gcgacgatgc cgaggttatg cgtgatgaag atgagcgcca gcccgtgcgc
     1681 ctgttgcagc tcgcgcaaca agttgaggta ctgggcttga atggtcaggt cgaggctggt
     1741 ggtgggctcg tcggcgatca gcaggcgcgg ttcgcaggag atgccgatgg ctccgacgat
     1801 gcgttggcgc atgccgccgg acatctcgtg cggaaattgc aggaggcgcg tggccggcga
     1861 ggcgatgcgc accgcccgca acaggtcgag cgcccggcgc caggccgtgc ggcgcttggc
     1921 gcgctcatgc acgcggatgg gctcggccac ctgatcgccg accgtgaaca gcgggttgag
     1981 cgaggccatg gggtcctgca ggatcatggc gatgcggcgc ccgcgcaggc ggcgcatctc
     2041 ggcttccgat ttttgcaaga ggtcttcgcc ttcgaacagc atgcggccgc cggcgatgcg
     2101 cgccgagggc ggcaacaggc gcagcacgct caaggcgagc gtgcttttgc cggaccccga
     2161 ctcgccaacg atgcccaagg tctcgcgcgc gccaatgctg aggctgaccc cgtcgaccgc
     2221 gcgcaccacg cgcgtgccgc gggccgagac gtagtgggtg gacaggttct ctattctgag
     2281 cagcggcgcg gcagccatca gtcacctcat gtccggcgca aggcgggctt cacagctgac
     2341 gcagctgggg gtcgagcttg acgcgcagcc agtcccccaa gaggttggcc gatagcacca
     2401 tcagcatgat gcacagtccc ggcaacacgg tcagccacca atagcctacc atcatgcctt
     2461 tcttgccgtc cgccagcatg aggccccagg ccggcttcgg cggcggtacc ccgacgccca
     2521 ggaacgacag ggccgcctcg gtgacgatca ccacccccag catcagcgtg cccagcacga
     2581 ttgcggaatt gaggacgttg ggcaggatgt ggcgacgcat gatggtccac ttcgagcacc
     2641 ccgccaccac ggccaggcgc acgaattcgc gttccttgag cgacaacact tcgccgcgaa
     2701 tgacgcgggc gtagcgggtc caatagaccg ccgccagaat gatgacgatg ttcatttcgc
     2761 tggggccgac gatggcggtc aggaagatgg caaaggtgag cgcgggcagc gccagccagg
     2821 tatcggtgat gcgcatgatg acctggtcga cccaaccccc gagatagccg gagatgatcc
     2881 cgagcagggt gcccagcgcg ccggccacca ccaccgcggt aaagccgacg accaccgaaa
     2941 cgcgggcgcc gaaaatcagc cgcgagagga catcgcgccc caaatgatcg gtgccgagca
     3001 ggtgttcgag gctgccgccg gcctgccagg cggggggccg aaagcgggcg gcaagcgagc
     3061 caatctccgg atcatgcggc gcgagtgcac ccgcgaacag ggcgacgagc gcaatgctcg
     3121 tcaggatcag cgtcggcagc aggggaaagt cggcgagccg aaaggaaaaa cgcggggata
     3181 gctcgacagc cggggtctca atggccatgg cgtcatcgct ccaatcggat gcgcggatcg
     3241 atcagcgcat aaaggatatc gatcagcagg ttcacaaaga cgatcatggc gccacccaac
     3301 agcaccacgg cctgcaccac cggaaagtcg cgaaaggtga tgccctcata aagcagccgg
     3361 ccgatgccgg gccaggcgaa caccgtttcc accaccacgg cgacgttgat cataatgacg
     3421 aggttgatgc cggcgagcgt cagcacgggg atcagcgcgt tcttgaaggc gtgtttggcg
     3481 atcacgcgcg gttcgggcaa gcccttgagc cgggccagct tgatgtactc ggagccgagc
     3541 acctcgagca tcgaggagcg cgtcaggcgc atatgcgcgg cggcaaaata ccagccgagc
     3601 gcgaaggccg gcatgaggag atgccacacc gtgcccgagc ccgacgaggg cagccagccc
     3661 agcgagaccg agaagatcag gatcaggacc aggcccaccc agaacgaggg cagcgacagg
     3721 ccgagtagcg agaaggtttt gccggcgcta tcccaaaagc caccgacgcg cacggcggcg
     3781 agaatgccgc tggggatgcc gataatgagg gagaaggcca tggccgccag cgccagcagc
     3841 aacgaattcg gcaggcgcgt tagataaagg tcgagcaccg gcgtgcggta atagaacgaa
     3901 tcaccgaaat cgcccgtcag caggctcgcc agaaaggtcc agtactgcac ccagatcggt
     3961 tgatcgaggc cgaagcgctg gcggatggca tcgatgtcgg cctgcggcgc gcccggttcc
     4021 acgagcagcg tggtgggatc gccggtcacc cgcacgaaga gaaagatcgt cagcgacaac
     4081 agcagtaagg agaccagcga gtagccgagc cgacgcagga tatagtgggt catgccgtct
     4141 ccttgcgcgc gtggtggcgt gggatctctc ggcgaatggc cggggtgcgg gcgcgccggt
     4201 cgcgggaccg ggacgggagc gctgttgccg tggggccgtg tcgcatgctg ctgccctccc
     4261 cgtcgggcta cttggtgacg accgtgacat cctcataggg cgccgaatag gcgtggccgg
     4321 cgatcaggcc caggcccgat tcgccgacgc gtggcccagc gccgttcaaa aacgccagct
     4381 gccacaggtg ggcatagatc gtgcgctcgt gcacgagctc ctggatcttg ttgagcaagg
     4441 ccccgcgctt ggccgcgtcg agttcgcttg cttggctgga aaacaaggca tcgatgtcgg
     4501 gatagctgcc ataaacatag gtgccgcccg tcaccacgaa ggattcgagg cgggtggcgg
     4561 cgttgccgaa agcgccgctc gcaccttgga tcaggttttt aaagctcttc tcggcgtagc
     4621 ccttgaaaaa cgcgacgcgt tccaggggcc gcagtttggc gcggatgccg atggcctgca
     4681 gattgttgag caccgcctcg gccaggttgg cgtaggaaac atcgcaatag tactcgccgg
     4741 catcaaagcc gttcgggtgt cctgcctcgg ctagaagttt tttggcaagc gtcggatcat
     4801 agctgggcag cggcggctgc caatactgct cgaaggtata gggaatgatg ctgccggtga
     4861 ttttggagtg cccgagcgtc agggcctgat tgatgccctt gtgatcgatg gccaggctgg
     4921 ccgcgcggcg gacgcgcgga tcgtgccagg gcgacttcgg atcccattgg tcgggaaagt
     4981 agagccagaa gggtgcctgg atgacggttg gcttcaggct caagcctttg ctgcgttcga
     5041 gctcctcggc cagctcgccg cggatcgaat aggcgatatc gacttcgccg cgtttcaggg
     5101 ccgcgagcct ggtcgcctcg tcggggatca cgcgcaagat cagccgttcg acgttcggct
     5161 ttttgcgcca gtactccggg aacgcctcca ggct
//
LOCUS       DASTMT010000249         1770 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_4069063, whole genome shotgun sequence.
ACCESSION   DASTMT010000249 DASTMT010000000
VERSION     DASTMT010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 1770)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1770)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1770
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4069063"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..720
                     /locus_tag="VFR00_05830"
     CDS             <1..720
                     /locus_tag="VFR00_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009494539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEU0158807.1"
                     /translation="DGYTVGVGNMGTQSAAPALYPNLKYDPATSFAQIGIANFTPQAI
                     VSKKDNPAKDLKEFIAYLKTNQDKLSYGHAGVGSISHVSGTLFNSQFGLKPALVAYRG
                     TAPALNDLVGGQIDYMVDQSLNVIPQIKAGTIKVYAIAAAQRLESLPEVPTSKEAGVD
                     FIFSAWNAMVAPKATPADIIAKLSAALNKALDDPATVKRYVELGSTAPEAAERGPEGL
                     QKLVESEMARITPVLKEAAAK"
     gene            788..>1770
                     /locus_tag="VFR00_05835"
     CDS             788..>1770
                     /locus_tag="VFR00_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013165377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase X"
                     /protein_id="HEU0158808.1"
                     /translation="MLAGEMGPARQWAIKHQLAVGDFFDAADFVRVGQAHIMADTESL
                     GASGVTWLEALAALPSAQRRVMVPTITDPRGLDFASYKRLKQTETMADLEARTIAAFE
                     ALGVLMTNTCINYQTILPPVRGEHMAFGDTGVVIYCNSVLGARSNFEGGPSALAAGLT
                     GRTPRYGYHLEEHRRASKHFAVRWRPRDLADWGALGGIVGRACNSYWEVPVLSGLETP
                     PNSDELKHFGAALASFGSVALFHIPGVTPEAATLAAAFAGRPSPAAVPIAAAEIAQFY
                     AGYAAGGDKVDVVVFAAPQLSLVELQQVAALLGDRRIHAGTTLIVATSPEI"
BASE COUNT          305 a          601 c          574 g          290 t
ORIGIN      
        1 gacggctaca ccgtcggcgt cggcaacatg gggacccagt cggccgcacc cgcgctctat
       61 cccaacctca agtacgatcc ggccacgagc ttcgcccaga ttggcatcgc caatttcacg
      121 ccccaggcta tcgtctccaa aaaagacaac ccggccaagg acctcaagga gttcatcgcc
      181 tatctcaaaa cgaaccaaga caagctgagc tatggccatg ccggcgtcgg ttcgatctcg
      241 cacgtctccg gcaccctgtt caattcccag ttcggcctca agccggcgct cgtcgcctac
      301 cgtggtaccg cgccggctct caacgacctg gtgggcggtc aaatcgacta catggtcgac
      361 cagtcgctga acgtcatccc gcagatcaag gccggcacca tcaaggtcta tgccattgcg
      421 gcggcgcagc ggctcgaaag cctgcccgag gtgcccacca gcaaggaggc gggcgtcgat
      481 ttcatcttca gcgcctggaa tgccatggtg gcgccgaagg caacgcccgc ggacatcatc
      541 gccaagctga gcgccgcgct gaacaaggcg ctcgacgatc ctgccacggt caagcgctac
      601 gtcgagctcg gcagcaccgc ccccgaggcc gccgagcgcg ggcccgaggg tctgcaaaag
      661 ctggtcgaaa gcgagatggc ccgcattacc cccgtgctca aggaggcggc ggcgaagtag
      721 cggtcttggc gcaacggtgc agctaggcgc gggcgggcgg atgcggctcg gggaagaaga
      781 acgggccatg ctggccgggg agatgggccc ggcgcggcag tgggcgatca agcaccagct
      841 cgcggtcggc gattttttcg atgcagccga tttcgtgcgc gtcggtcagg cccacattat
      901 ggcggacacg gagagccttg gcgcctccgg cgtcacctgg cttgaggccc tcgccgcact
      961 gccgagcgcg cagcgccggg tgatggtgcc gaccatcacc gatcctcgtg gccttgattt
     1021 tgccagctac aagcggctga agcagaccga gacgatggcc gatctcgagg cgcgtacgat
     1081 cgccgccttc gaggcgctcg gcgtgctgat gaccaacacc tgcatcaact accaaaccat
     1141 cctgccgccg gtacgcggcg agcacatggc cttcggcgat accggcgttg tgatctactg
     1201 caacagcgtc ctgggcgcgc gcagcaattt tgagggtgga ccctcggccc tggccgcagg
     1261 gttgacgggt cgcaccccgc gttacggcta ccatcttgag gagcatcgcc gcgccagcaa
     1321 gcacttcgcc gtgcgctggc ggccgcgcga cctcgccgat tggggtgcgc tcggcggcat
     1381 cgtcggtcgt gcctgcaata gctactggga ggtgcccgtc ttgagcggct tggagacgcc
     1441 gccaaattcc gacgagctca agcacttcgg cgccgccctg gcgagtttcg gctcggtggc
     1501 tctgttccac atacccggcg tgacgccgga ggcggccacg cttgccgcgg cttttgcggg
     1561 gcgccctagc cccgccgcgg taccgatcgc ggccgccgag atcgcgcagt tctacgccgg
     1621 ttatgccgcg ggcggcgaca aggtcgacgt cgtggtgttc gccgcgccgc agctgtcgct
     1681 ggtcgagctg cagcaggtcg cagctttgct cggggaccgg cgcattcacg ccggtaccac
     1741 cttgatcgtg gcgacctcgc ccgagatcaa
//
LOCUS       DASTMT010000250         5189 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_1120036, whole genome shotgun sequence.
ACCESSION   DASTMT010000250 DASTMT010000000
VERSION     DASTMT010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 5189)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5189)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5189
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1120036"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..344
                     /locus_tag="VFR00_05840"
     CDS             <1..344
                     /locus_tag="VFR00_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015927938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0158809.1"
                     /translation="AHKAFIEAYRAKYNDAPRLGSVLGYVVAYMMKDMLDKAGSTETE
                     KMLASLRGGTFKTIVGDVVMRAIDQQSSMGAWVGETTKHGKVGTMKNWTYEDGSKFMF
                     SEAEVKAARKE"
     gene            466..2352
                     /locus_tag="VFR00_05845"
     CDS             466..2352
                     /locus_tag="VFR00_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019196179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0158810.1"
                     /translation="MDLAFLFVQALNGLASASTLFIISAGLTLVFGVTRIVNFAHGSF
                     YMLGAYLGVSIIPRLLDVASSLPMFLVGVLAAAAGVGLLGVAMEVLLLRRIYRVPELF
                     QLLATFGVVLVVQDIVLKIWGPLDILGPRAPGLGGAVTIFGHRFPAYELFLIAMGPLM
                     LGLLWLAMHKTRFGVLVRAATQDREMVAALGVNQALLFTGTLFLGAALAGLAGALQIP
                     KAAANPQMDIAVIAEAFVVTVVGGMGSITGAFLAALIIGELQAFGILIFPKVTLVLVF
                     LLMALVLVARPSGLMGRPEATPQRAVLPEGIVALARFGKREAVALAAALVALLLLPLL
                     GGAYLTKVAIEVLTFALAAFSLNFLVGVGGIVSFGHAAYFGLGAYAAGLLVTRLGLPM
                     EAALVAAPIAAGLGAAIFGFFIVRLSGIYLAMLTLAFAQITYAVAFQWVEVTGGDNGV
                     VGVWPSAWAASRQAYYAFTLVVAAVAILLLGRIIYAPFGYTLRAARDSAVRADAIGID
                     VRTHRWLAFTLAGAAAGLAGGLFAFSKGSIDPTLISIPMSVDLLIMILAGGIQTITGP
                     LVGALFFHSIKDTFMPLTDYWRLLLGLAIIVVVLAFPRGIVGGLAQLRERFASPASER
                     RGAT"
     gene            2349..3113
                     /locus_tag="VFR00_05850"
     CDS             2349..3113
                     /locus_tag="VFR00_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009763148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0158811.1"
                     /translation="MSLLSVEALQKSFGGVVAANNVSFALAGGHLIAIIGPNGAGKST
                     IFNMLGGQLKPDRGHVRLAGADITGLAPRQIWRQGVGRTFQIAQTFLSMTVLENVQMP
                     LVSLARRTRACWADSSSLYRAEALELLARVGVEAQADRPCSELAYGDVKRVELAIALA
                     SSPKLLLMDEPTAGMASSERSQLMRLVKSIARERGIGVLFTEHDMDAVFDHADSVLVL
                     VRGEIIARGRPAEVRADQRVRQLYLGHAAPQPPPQA"
     gene            3125..3847
                     /locus_tag="VFR00_05855"
     CDS             3125..3847
                     /locus_tag="VFR00_05855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009398857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0158812.1"
                     /translation="MQQGTSILEVEGLNAAYGRAQILFGVSLRLARGEILALMGRNGA
                     GKSTTLKAIMGMVPASAGSLRFAGHDIRHLPTYRIARLGLGYVPEDRRIFTELTVFEN
                     LEVGRKAEQPTGPAGWTPERLFSIFPNLADMRGRQASQMSGGEQQMLSIARTLMGNPQ
                     AVLLDEPTEGLAPVIVEEMVAAVARMKREGLAVLLSEQNAAFAAAVADRVAIIAKGVV
                     RHEGPMADLSRDAALREAYLGL"
     gene            3973..4248
                     /locus_tag="VFR00_05860"
     CDS             3973..4248
                     /locus_tag="VFR00_05860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEU0158813.1"
                     /translation="MDQRPRDLPPKSDWDRLPTTDDASWSVLPILIVVALIAVGAWLL
                     FANSGTSPTTTSETAPVTAPSPRPAQPPATTPTTPPANTAPAPATKP"
     gene            complement(4344..>5189)
                     /locus_tag="VFR00_05865"
     CDS             complement(4344..>5189)
                     /locus_tag="VFR00_05865"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HEU0158814.1"
                     /translation="KQSAGVIPHDMAPELFANFSSINPMTRTVADCALMLETMAGAHA
                     CDPYSYGAPARGFIAAARPEGSLKGLRVAWRPLMANSVIDREVLELCERAAITLGELG
                     ASVEPMDDDLEPVEPAWFAYASAMWNARFAELLPRWSNRLSPTFVRQMELGKDTTATA
                     VGQALLRRSALFRQVQGWFERFDVILTPTLTRTALPIEERLFEPIEIEGTPVDTVRRA
                     WYPYTHPFNLTGHPAVSLPAGFHTDGLPVAVQLIGRRHEDWRLLRVAAHFEAARGVSP
                     RRPTL"
BASE COUNT          779 a         1791 c         1709 g          910 t
ORIGIN      
        1 ccgcccacaa ggccttcatc gaggcctacc gcgcaaaata caatgacgcc ccgcgcttgg
       61 gctcggtgct gggctacgta gtggcctata tgatgaagga catgctggac aaggccggct
      121 cgacggagac cgaaaaaatg ctcgccagtc tgcgcggtgg aaccttcaaa accatcgtcg
      181 gggacgtggt catgcgcgcc atcgatcagc aatcgagcat gggggcgtgg gttggcgaga
      241 ccaccaagca cggcaaggtc ggcaccatga aaaactggac ctacgaggac ggcagcaaat
      301 tcatgttctc ggaagccgag gtgaaggccg cacgcaaaga gtagagggcg ccggagcggt
      361 cctgtcatgg ctcggcaccc cgagcacggc gcctcgcgca ggcgcgctta cccctgccac
      421 ctcccaggag gtgccgcacc gcctcggccg cggcacggtt gagcgatgga tctcgccttc
      481 ctgttcgtgc aggcattgaa cggattggca agcgcgtcga cgctgttcat catctccgcc
      541 ggactgaccc tcgtgttcgg cgtaacgcgc atcgtcaatt tcgcgcacgg ctccttttat
      601 atgctgggcg cctacctcgg cgtcagcatc attccaaggc tgctcgatgt cgccagcagc
      661 ctgccgatgt ttctcgttgg cgtcctcgcc gccgccgccg gcgtcggtct gctaggcgtt
      721 gccatggagg tcctgctgct gcggcggatc taccgggtgc ccgagctgtt ccagctgttg
      781 gccaccttcg gcgtggtgct cgtggtgcag gacatcgttc tcaagatctg gggcccgctc
      841 gatatcctcg gcccgcgcgc gccggggctc ggcggcgccg tgacgatttt cgggcatcgc
      901 tttcccgcct acgagctgtt cttgattgcc atggggccgc tgatgctcgg cctgctctgg
      961 ctcgccatgc acaagacgcg ctttggcgtg ctggtgcggg ccgcaacgca ggatcgcgaa
     1021 atggtggccg cgctcggggt caatcaggcg ctgctgttca ccggcacgct gttcttgggg
     1081 gcggcgctgg cgggcctcgc cggcgcgctg cagatcccga aggcggcggc caatccgcaa
     1141 atggatattg cggtgatcgc ggaggctttt gtcgtcaccg tggtcggtgg catgggatcg
     1201 atcacgggag ccttcctggc cgcgctcatc atcggcgagc tgcaggcctt cggcatcctg
     1261 atctttccca aggtgacctt ggtgctggtg ttcctgctga tggcgctggt gctggtggca
     1321 aggccctctg gcctgatggg tcggccggag gccaccccgc agcgggcggt gctgcccgaa
     1381 ggcatcgtcg ctctggcgcg tttcggcaag cgcgaggccg ttgccctggc cgcggcgctc
     1441 gtcgccttgc tgctcctgcc cctgctcggt ggggcctatc tgaccaaggt ggcgatcgag
     1501 gtcctcacct ttgcgcttgc cgccttcagc ctcaacttcc tggtcggcgt cgggggcatt
     1561 gtcagcttcg gccatgccgc ctatttcggc ctcggggcct acgccgcggg actgctggtc
     1621 acccgcttgg gcctgcccat ggaagccgcg cttgtcgccg caccgatcgc cgccggcttg
     1681 ggcgcggcca tcttcggctt tttcatcgtg cgcctgagcg gcatctatct cgccatgctg
     1741 acgctggcct tcgcccagat cacctacgcg gtggccttcc agtgggtcga ggtgacgggc
     1801 ggcgacaacg gcgtggtggg cgtgtggccc tcggcttggg cggcaagccg ccaagcctac
     1861 tacgccttca cgctggtggt ggccgcagtg gcgatcctgc tcctgggccg catcatttac
     1921 gcacccttcg gctacacgct gcgcgcggcg cgagattcgg cggtgcgcgc cgatgccatc
     1981 ggcatcgacg tcaggaccca ccgctggctc gctttcacgc tcgccggcgc cgcggccggc
     2041 ttggccggcg gcctgtttgc cttctccaag ggctcgatcg acccgaccct gatctcgatc
     2101 ccaatgtcgg tcgatctgct gatcatgatc ctggcgggcg gcattcagac catcaccggt
     2161 cccctggtgg gcgcgctctt ctttcattcc atcaaggaca cgttcatgcc gctcaccgat
     2221 tattggcggc tcctgctcgg cctcgccatc atcgtggtcg tgctcgcctt cccgcgcggt
     2281 atcgtcggtg gcctcgcgca gctgcgcgaa cgtttcgcct ccccggcgag cgagcgaagg
     2341 ggagcgacat gagcctgttg tcggtcgagg ctctgcaaaa atccttcggt ggcgtagtgg
     2401 cggccaacaa cgtgagcttc gcgctggctg gcggacacct catcgccatc atcggtccca
     2461 acggcgcggg caagtccacg atcttcaaca tgctgggcgg acagctgaaa cccgaccgcg
     2521 gccacgtcag gctggccggg gccgacatca ccggactggc cccgcggcag atctggcgcc
     2581 agggcgtcgg ccgcaccttc cagatcgcgc aaaccttcct gtcgatgacc gtgctcgaaa
     2641 acgtgcagat gcccttggtc agcctggccc gacgcacgcg ggcctgctgg gccgacagta
     2701 gcagcctcta tcgcgccgag gcgctcgagc tcctcgcccg cgtcggcgtg gaagcgcaag
     2761 cggaccggcc gtgcagcgag ctcgcctacg gggacgtcaa gcgcgtcgag ctcgcgattg
     2821 cgctggcgag cagccccaag ctgctgctga tggatgaacc cacggcgggc atggcgtcga
     2881 gcgaacgcag ccagctgatg aggctcgtca aatcaatcgc gcgcgaacgc ggcatcggcg
     2941 tgctctttac cgaacatgac atggatgcgg ttttcgacca cgccgatagc gtgctcgtgc
     3001 tggtgcgcgg cgaaattatc gcccgcggcc ggccggccga ggtgcgcgcc gaccagcgcg
     3061 ttcgccagct ctatctgggc cacgcggctc cccagccgcc gccacaggct tgaggagcgc
     3121 acccgtgcag caggggacca gcattctcga ggtcgaaggc ctcaatgccg cctacggccg
     3181 ggcgcaaatc ttgttcgggg tgtccttgcg cttggcgcgt ggcgagatcc tggcgctgat
     3241 gggcagaaac ggcgccggca agtcgaccac gctgaaggcc attatgggca tggtaccggc
     3301 cagcgccggc agcctccgct ttgccggcca cgacatcagg cacctgccca cctaccgcat
     3361 cgcccgcctt ggcctcggct acgtgccgga ggatcgcagg attttcaccg agctcaccgt
     3421 gttcgagaac ctcgaggtcg gccgcaaggc cgagcagccg accgggcctg cgggctggac
     3481 acccgagcgg ctgttttcca tttttcccaa cctcgccgac atgcgcggcc gccaggccag
     3541 ccagatgtcg gggggtgagc agcagatgct cagcattgcc cgcacattga tgggcaaccc
     3601 gcaagccgtc ctgctcgatg aaccgaccga agggctggcg cccgtcatcg tcgaggagat
     3661 ggtcgccgcc gtcgcccgca tgaagcggga aggcctggcc gtgctgctgt ccgagcagaa
     3721 cgcggccttt gccgcagccg tggccgaccg cgtcgccatt atcgccaagg gcgtcgtgcg
     3781 ccacgaaggc ccgatggccg acctcagccg cgacgccgcc ctgcgcgagg cctatctggg
     3841 gctttagggc ccgcccgcgc gcggggtgcg gccggccgcc accgctgagc tcaagccccc
     3901 ggggagcgcg gaacatgtct tgcaacgggg cgttcctccc gcgaccagga caccgcatcc
     3961 cggaggccca agatggatca acgaccgcgc gatctcccgc caaagtccga ttgggatcgc
     4021 ctgcccacca ccgacgacgc cagctggagc gtgctgccca tccttattgt ggtcgccctc
     4081 attgccgtgg gcgcttggct gctcttcgcc aatagcggaa cttcgcccac caccacctcc
     4141 gaaacggcac ccgtgaccgc gcccagccca cggccggcgc agcccccggc aacgacgccg
     4201 acgacgccgc ccgccaatac cgccccggcg cccgccacca agccctgagg cgcgaaagct
     4261 tttctttgtc ttcgcctcga tgtctttcca ggccccgcgt gtgcggggcc tctttttgcc
     4321 tgtgtggcgg gtgcggatgg gccttaaagc gtcggccggc gcgggctcac gccgcgggcc
     4381 gcttcaaagt gggccgccac gcgcagcaag cgccagtcct cgtgccggcg accgatcagc
     4441 tgcaccgcaa cgggcaaccc atcggtgtgg aacccggcgg gcaggctgac ggcgggatgc
     4501 cccgtcaagt tgaaggggtg cgtgtagggg taccaggcgc ggcgcacggt atcgaccggc
     4561 gtgccctcga tttcgatcgg ctcgaacaag cgctcctcga tcggcaaggc cgtgcgcgtc
     4621 agcgtcggcg tcaggatcac gtcgaagcgc tcaaaccagc cctgcacctg acgaaacagc
     4681 gcgctccgcc gcaatagcgc ctggccgacg gcagttgcgg tcgtgtcctt gccaagctcc
     4741 atctggcgca cgaacgtcgg gctcagccgg ttgctccagc gcggcagcag ctcggcaaag
     4801 cgcgcattcc acatggcgct ggcgtaggcg aaccaggccg gctcgacggg ttcgaggtca
     4861 tcgtccatcg gctccacgct ggcgccgagt tcgcccagcg tgatcgccgc gcgctcgcaa
     4921 agctcgagca cttcccggtc gatcacgctg ttggccatca gcggacgcca ggccacgcgc
     4981 aagcccttga ggctgccctc cggccgcgcc gcggcgatga agccgcgcgc cggcgcaccg
     5041 tagctataag ggtcgcaggc gtgcgccccg gccatggtct ccagcattaa ggcgcagtcg
     5101 gcgaccgtgc gcgtcatcgg gttgatgctc gagaagttgg cgaacagttc cggcgccatg
     5161 tcgtgaggga tgacgcctgc cgactgctt
//
LOCUS       DASTMT010000251         2270 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_2427570, whole genome shotgun sequence.
ACCESSION   DASTMT010000251 DASTMT010000000
VERSION     DASTMT010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2270)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2270)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2270
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2427570"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..562)
                     /locus_tag="VFR00_05870"
     CDS             complement(<1..562)
                     /locus_tag="VFR00_05870"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU0158815.1"
                     /translation="MGKIVLVPPSADAGTIARDLAPGGFELVLLSHTAPEFEAALAGM
                     DYMVCYPNVAMPDAFYRAAPRLKLVQLLSAGYDNVDLEAARRAKVPVCNNGGANATSV
                     AEHSLMLMLAVARKVIWQHGNVSAGRWRGNGPAPRMYELNGKTLGIVGFGTIGKKTAR
                     LAQAFGMKVQYYDIRRLSEEVEDALGV"
     gene            complement(1106..1360)
                     /locus_tag="VFR00_05875"
     CDS             complement(1106..1360)
                     /locus_tag="VFR00_05875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0158816.1"
                     /translation="MTSSKLRVSKTSALILGAAVLLTGPLSSIALAHGHHHHRHGVRI
                     LIGGGPGIYGYGGSCRWLKLRALETGSPYWWKRYRFCMYG"
     gene            1664..2224
                     /locus_tag="VFR00_05880"
     CDS             1664..2224
                     /locus_tag="VFR00_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018183999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU0158817.1"
                     /translation="MQKAQDAQLIARIAVQDQAALRGLYARHQARVFRFLLRLVRQEA
                     IAEELTNEVFMEAWRHAASFEGKSSAATWLLSIAHHRAVSVLRRRREESWNEEEAAEI
                     ADAEDDPEVQLQKTDKASLLRRCLEALSPEHREVIDLVYYHEMSIGEVSSIVGIPEST
                     VKTRMFYARKRMSELLKTAGVDRGWP"
BASE COUNT          413 a          744 c          764 g          349 t
ORIGIN      
        1 gcacgccgag cgcatcctcc acctcctcgc tcaggcgcct gatgtcgtaa tactgcacct
       61 tcatgccgaa cgcctgggcc agccgcgcgg tctttttgcc gatggtgccg aagccgacga
      121 tacccagcgt tttgccgttg agctcataca tgcgcggtgc cggtccattg cctcgccaac
      181 gcccggctga gacattgcca tgctgccaaa tcactttgcg cgccacggcg agcatcagca
      241 tcaggctgtg ctcggccacc gaggtggcat tggcgccgcc attgttgcag acggggacct
      301 tggcgcgccg tgccgcctcc aagtcgacgt tgtcgtagcc tgcgctcagc agctgcacga
      361 gtttcagccg cggggcggcg cggtagaagg catccggcat cgccacgttg ggatagcaca
      421 ccatgtagtc catgcccgcc agcgccgcct cgaactctgg ggccgtgtgc gacagcagca
      481 ccagttcgaa tccgcccggc gccaagtcgc gcgcgatcgt gcctgcgtcc gccgacggcg
      541 gcaccagaac tattttgccc attgctaccc ctccgatcgg gtcgcttgcc tgaagcggcg
      601 cccaccaccg gggattatgc accaatcccc gcgccgggcg ctaaccggct cggcttggca
      661 gccccgagcg gcgcgcaacg gtcgccacgc cggtcaaggc gcgggtagcc ctaagcgggg
      721 gagccgtgcg cggacgtgcc tgccgcgccc aaggggaaga cgcaacgccc cctcaagcct
      781 tggcggcggc aaacgccaac gcctgcagcc acccttgcag gcgatcgacg cagccaaagg
      841 ccgtgccgcc ggcgaatgac ctcgcccacc gcatgaacgg cacgtggttt ggcgcagcac
      901 acgcgctcgc ggcaaaacat gccggggggc aagggccgcg acgagcaata gggttgccta
      961 ggccacacag gcccggcgct tcggcgccgg cccgcgcgcg atgcgcacgc aggacagacg
     1021 gcagcggcgc gccccgctgc cgcgacgggg gagcggcaac ggggcggcga gcgatgaacc
     1081 gatcgagcca acggcccagc ctcgctcaac cgtacatgca gaagcggtag cgcttccacc
     1141 agtacgggct tccagtttca agcgcgcgca gcttcagcca gcggcaggag ccgccataac
     1201 cgtagatgcc ggggccgcca ccgatcagga tgcgcacgcc gtggcggtga tgatgatggc
     1261 catgcgccag cgcgatggag ctcaagggcc cggtcagcag gacggcggcg ccgaggatca
     1321 gggcagacgt cttagacacg cgaagcttgg acgaggtcat gactgcgaac tcctagggtt
     1381 tgagtgtcgg ccgggctgcg ccgaccgtgt gccctctagt cgcggcggcg gcggaaaagg
     1441 ttcaatgcca cccaaaagaa ttttgccgtc gccccccggc gggtggcgtt cgggcggtcg
     1501 atggcggcgg taatcccaag cttgcccgcg cgtgctaacc taagcggtcg aaatcggcgc
     1561 cggggctcga gcaggcctgg ccaatgcttt ttttgcaaag tgccttgaac ctttgaccat
     1621 gtccgagcga ccaaaggagt gccagacgcc tgacggcgct gcgatgcaaa aggcgcagga
     1681 tgcgcaactc atcgcgcgca tcgccgtcca ggatcaggcg gccctgcgcg ggttgtacgc
     1741 gcgacatcag gcccgggtgt ttcgcttcct gttgcgcctc gtgcgccagg aggcgatcgc
     1801 cgaggagctg accaacgagg tttttatgga ggcctggcgc catgccgcca gcttcgaagg
     1861 caagtcgagc gcggcgacct ggctcttgtc gatcgcgcat catcgtgccg ttagcgtctt
     1921 gcgccggcgc agggaagaga gttggaacga ggaggaagcc gccgagattg ccgatgccga
     1981 ggacgacccg gaagtgcagc tgcagaagac cgacaaggcg tcgctcctgc gccgctgcct
     2041 ggaggccctg tcgcccgagc accgggaggt catcgacctc gtttactatc acgagatgtc
     2101 gatcggcgag gtgagcagca ttgtgggcat acctgagagc acggtaaaga cgcgcatgtt
     2161 ttacgcccgc aagcggatgt cggagctgtt gaaaaccgcg ggggtggacc gaggttggcc
     2221 atgagcgaag acgagacgac attctccgag cgcgaagaga tcgagatgct
//
LOCUS       DASTMT010000252         4510 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_2757098, whole genome shotgun sequence.
ACCESSION   DASTMT010000252 DASTMT010000000
VERSION     DASTMT010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4510
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2757098"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            290..1324
                     /locus_tag="VFR00_05885"
     CDS             290..1324
                     /locus_tag="VFR00_05885"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase family protein"
                     /protein_id="HEU0158818.1"
                     /translation="MQFAKLVARVLALTIAVAGLGLTGLRMALSHQSDSELLAADLAQ
                     TLERASDRDRRLAEKGLLAGSPILIRIFKAEFELELWMRKGERFELFATYPICYWSGT
                     LGPKLHEGDKQAPEGFYAVGLNQIHRRGRWPRSLNIGYPNALDRAHARTGSLILVHGG
                     CTSTGCFAMTNPVMAEIYALAEQALQQGQDRIPIHVFPFRMTDANLAARSVTHGEWNA
                     FWLNLKLGYDLFETTRVPPKVGICNKQYALSAGDLADGSGPPTGLATPCAESGGGGAV
                     PLVTAETEAAAEQESLAAKPRKVASKPRHRYAARHARKAYAASRRARTAAPIETSSIS
                     GPISGGEPVH"
     gene            complement(1335..2471)
                     /gene="pqqE"
                     /locus_tag="VFR00_05890"
     CDS             complement(1335..2471)
                     /gene="pqqE"
                     /locus_tag="VFR00_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018149 - peptide cross-linking [Evidence
                     IEA];
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone biosynthesis protein
                     PqqE"
                     /protein_id="HEU0158819.1"
                     /translation="MSDAAALDHETVAGLSAPLGLLAELTHRCPLQCPYCSNPLALER
                     VNAELSTEAWCDVLAQAAELGVLQLHLSGGEPTLRRDLARIVAQATRVGLYTNLITAG
                     VLLTRDRLEDLVANGLEHVQISIQDSNAANADRIAQYAGGHEKKLEVARWATALKLPL
                     TLNAPIHRQNIDSLPAIIELALALGAQRLEVAHVQYYGWALKNRQALMPTRAQVLRSA
                     ELVAAARDRLKGVLVIDFVVPDYYARRPKPCMGGWGRGIITITPSGKVLPCHAAQTIE
                     GLKFENVRERSLREIWLQSEAFAAFRGTGWMREPCRSCEFRELDWGGCRCQALAFTGS
                     AANADPACEKSSYHAAFTAIAGAESEAPAQAFAFRRPSPAPGGR"
     gene            complement(2468..2770)
                     /gene="pqqD"
                     /locus_tag="VFR00_05895"
     CDS             complement(2468..2770)
                     /gene="pqqD"
                     /locus_tag="VFR00_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone biosynthesis peptide
                     chaperone PqqD"
                     /protein_id="HEU0158820.1"
                     /translation="MIKASDPKRALIAAASVPTLPRHIKLRHDATRNRWTILAPERVL
                     TPDAIALDILRLCDGERSVEAIAEVLAGQYSAPKARILTDVTNLLQELADKGVITA"
     gene            complement(2763..3500)
                     /gene="pqqC"
                     /locus_tag="VFR00_05900"
     CDS             complement(2763..3500)
                     /gene="pqqC"
                     /locus_tag="VFR00_05900"
                     /EC_number="1.3.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline-quinone synthase PqqC"
                     /protein_id="HEU0158821.1"
                     /translation="MSELLTPDELEAAIRQVGAERYHDKHPFHRLLHGGKLNKGQVQA
                     WALNRYCYQAAVPRKDAALMGRVADRELRRAWVHRLLDHDGYAGEEGGIERWLVLTDV
                     LGLERAYVTSMQGALPATRFATEAYVRFVVQEPVLDAVASSLTELFAPQIHQERISGM
                     LENYAFIGERAMQYFRRRLDQANRDADFALDFVKRHATTAAQQTACIEAVRFKCNVLW
                     AQLDALNHAYVVPGLIPPGAFRPEAHD"
     gene            complement(3497..4408)
                     /gene="pqqB"
                     /locus_tag="VFR00_05905"
     CDS             complement(3497..4408)
                     /gene="pqqB"
                     /locus_tag="VFR00_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003610947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone biosynthesis protein
                     PqqB"
                     /protein_id="HEU0158822.1"
                     /translation="MLIKVLGSSAGGGLPQINCNCRNCADARAGSRAVRRRTQSSVAV
                     SRDGSDWTLLNASPDLRQQIDATPQLWPRPEGGRRSSPIVSIVLTNADVDHVAGLLSL
                     REGVSFTLYGSASVLAALAANPIYAVLDRAHVPRVTLALESKLVLAGGLCLEAFAVPG
                     KIALYLETAAAEFGSRPGDTLGLKISDPESGAAFFFIPGCAAVDATLAARLRGAPLVL
                     FDGTLYTDDEMIVQGLSSKTGQRMGHLAMAGPSGAITAFAGVEVGRRVFVHMNNSNPA
                     LREDSPERASVEAAGWEIAWDGMEIEL"
BASE COUNT          800 a         1446 c         1494 g          770 t
ORIGIN      
        1 gcgctcgcgc gcggtcccgt ccgccgcctt gaggcgatat ttcatgtccc agatctgctg
       61 ggaaatggcc ggcagggaag acggagcgtg gctcatctcg ttcatggtat gttctagcag
      121 attttcctag ttggtgcaac aatgctaaga agcggttgca ggtttttggc ggtcgcctgg
      181 cgatggtggc gcggtcgcca tgcgcaggca ggggaagaac cgcggtcgag acggggacat
      241 agcgtggcgg ggaccgatgc gcggccttgc cggggggaca aggctcggga tgcaatttgc
      301 caaactcgtt gcgcgcgtgc tcgcgctcac catcgccgtc gccgggctcg gcctgacagg
      361 cttgcgcatg gcattgagcc atcagagcga cagcgagttg ctcgctgcgg atttggctca
      421 aaccttagag cgagcgagcg atcgcgatcg caggctcgcc gagaagggcc tgctggccgg
      481 tagcccgatc ttgatccgga tcttcaaggc agagttcgaa ctggaacttt ggatgcgcaa
      541 gggcgagcgc ttcgagctct ttgcgaccta tccgatctgc tactggtcgg ggacgctggg
      601 accgaagttg cacgagggcg acaagcaggc acctgaaggc ttctacgccg tcgggctcaa
      661 tcagatccat cgccgtgggc gctggccgcg ctctctcaat atcggctatc ctaatgcgct
      721 cgatcgtgcg catgcacgta cgggctcgct catcctcgtg cacgggggct gcacctcgac
      781 gggctgtttt gcgatgacca acccggtgat ggccgagatc tacgccctgg ccgaacaggc
      841 cttgcagcaa ggacaagacc gcatccccat ccacgttttt ccatttcgca tgacggatgc
      901 caacctcgcg gcgcgcagcg tcacgcacgg cgaatggaac gctttctggc tcaatttgaa
      961 gctcggctat gatctgtttg aaacgacgcg cgtgccgcca aaagtgggca tctgcaacaa
     1021 acaatatgcc ttgagcgctg gtgacctggc ggacggctcg ggtccgccga cgggcctggc
     1081 cacgccgtgc gcggagagcg gcggcggcgg cgccgtgccc ttggtgacgg cagaaacgga
     1141 agccgccgcc gagcaagaga gcttggccgc caaaccgcgc aaggtcgctt ccaagccgcg
     1201 gcaccggtat gccgcccgcc atgcccgcaa ggcctatgcg gcctcccggc gcgcgcgcac
     1261 cgccgccccg attgagacct catccatatc cgggcctatt tccggcggcg agccggtgca
     1321 ctgagccggc gggctcagcg cccgcccggc gccggcgagg gccgtctgaa ggcgaatgcc
     1381 tgcgccggtg cctctgactc cgccccggcg atcgcggtaa aggccgcgtg gtaactggat
     1441 ttctcgcagg ccggatcggc gttggcggca gagccggtga aggcgagagc ctggcagcgg
     1501 cagccgcccc agtcgagctc gcgaaattcg cacgagcggc agggctcgcg catccagccg
     1561 gtgccccgga aggctgcaaa agcctccgac tgcagccaaa tttcgcgcag actgcgttca
     1621 cgcacgttct cgaacttgag cccttcgatg gtctgggcgg catggcaggg cagcaccttg
     1681 cccgaaggcg tgatggtgat gatgccgcgg ccccaaccgc ccatgcaggg cttgggccgc
     1741 cgggcatagt agtccggcac gacgaaatcg atgacgagca cccccttaag ccggtcccgc
     1801 gccgccgcga cgagctccgc gctccttagc acttgggcgc gtgttggcat cagggcttgc
     1861 cggttcttga gcgcccagcc gtaatactgc acgtgtgcaa cctcgaggcg ctgggcgccg
     1921 agggcgagcg cgagctcgat gattgccggc aagctgtcga tgttctggcg gtggatcggg
     1981 gcgttgagcg tcagaggcag cttgagtgcc gtcgcccaac gcgccacctc gagctttttt
     2041 tcatgtccgc ccgcatattg ggcgatgcgg tcggcgttgg ctgcattgct gtcctgaatg
     2101 gaaatttgca cgtgctcaag cccgttggcg acgaggtcct cgaggcggtc ccgggtcaac
     2161 agaaccccgg cggtgatcag gttcgtgtaa aggccaacgc gcgtcgcttg ggcgacaatc
     2221 cgggcaaggt cgcgccgcag cgtcggctcc ccgcccgaga ggtggagctg cagaacgcca
     2281 agctcggcgg cctgcgccag gacatcacac cacgcctcgg ttgagagttc ggcgttgacc
     2341 cgctccaagg cgagcgggtt tgagcaatag ggacattgca gcgggcaccg gtgcgtaagc
     2401 tccgcgagca ggccgagggg tgcggataga cccgctacgg tttcgtggtc gagcgcggca
     2461 gcgtcactca cgccgtgata acccctttgt cagccagctc ctgcagcaga ttggtgacgt
     2521 ccgtcaggat gcgcgctttc ggcgcactat attggccggc gagcacctcc gcgattgcct
     2581 ccacgctgcg ttccccgtcg cacaagcgca aaatatcgag cgcaatcgca tctggagtga
     2641 gcaccctttc gggcgccagg atggtccagc gattgcgtgt cgcgtcgtgg cgcagcttga
     2701 tatgacgcgg cagcgtcggc accgaggccg ccgcgatcag cgcgcgcttg gggtcgctag
     2761 ctttaatcat gcgcctccgg gcgaaaggct cctggcggga tcaggccggg gaccacatag
     2821 gcatggttga gagcatcgag ctgcgcccat agcacgttgc atttgaagcg caccgcctcg
     2881 atgcacgcgg tttgctgggc tgccgtggtg gcatgacgct tgacaaaatc gagcgcgaaa
     2941 tcggcgtcgc ggttggcctg atcgagccgg cgccgaaaat actgcatggc ccgctcgccg
     3001 atgaaggcat aattctccag catgccggaa atgcgctcct ggtgaatctg cggcgcgaat
     3061 agttccgtca acgatgaggc caccgcatcg agcaccggct cttgcacgac aaaacgcaca
     3121 taagcctcgg tggcaaagcg ggtggcgggt agcgcgccct gcatggacgt cacataggcg
     3181 cgctccagcc cgagcacatc ggtcaacacc agccagcgct cgatgcctcc ttcctcgcca
     3241 gcatagccgt cgtgatccaa cagacgatgg acccaggcgc ggcgcagctc gcggtcggcc
     3301 acgcggccca tgagcgcggc atccttgcgc ggtacggcgg cctggtagca atatcggttg
     3361 agcgcccagg cctggacttg gcccttgttc agcttgccac cgtgcagcag gcgatggaaa
     3421 ggatgcttgt cgtgatagcg ctcggctccg acctggcgga tcgccgcctc gagttcatcg
     3481 ggcgttagga gctcgctcac agctcaatct ccatgccgtc ccaggcgatc tcccaacccg
     3541 cggcctccac gctggcccgc tccgggctgt cctctctgag agcgggattg gagttgttca
     3601 tgtgcacgaa aactcgccgt ccgacctcga cgcccgcgaa ggctgtgatc gcgccgctgg
     3661 gaccggccat ggccaagtgg cccatccgct ggcccgtctt cgaggaaagg ccttgcacaa
     3721 tcatttcgtc atcagtgtag agcgtgccgt cgaacagaac caggggtgcc ccgcgcagcc
     3781 gcgccgccaa ggtggcatcg accgccgcgc accccgggat gaagaaaaag gcggcgccgc
     3841 tctcgggatc ggaaatcttg agcccgagcg tatcaccggg cctgctgccg aattccgctg
     3901 cggcggtctc aaggtaaagg gcgatcttgc ccggaacagc aaaggcctcg aggcaaagtc
     3961 cgccggcgag cacgagcttg ctctcgagcg ccaaggtgac ccgcggcaca tgggcacgat
     4021 ccaacaccgc gtagatcggg ttggctgcca gcgccgccag cacgctggcg gagccataaa
     4081 gggtgaagct caccccctcg cgcaggctca gcaggcccgc cacgtggtcg acgtcggcgt
     4141 tggtgaggac gatcgagaca attggactgc tgcgccggcc gccctcaggc cgcggccaaa
     4201 gctgcggcgt ggcgtcaatt tgctgccgca gatcggggga cgcattgagg agggtccagt
     4261 cgctaccgtc gcggctcacc gccaccgagg actgcgtgcg tcggcgcacc gcccgactgc
     4321 cagcgcgggc gtcggcacaa ttgcggcaat tgcaattgat ctgcggtagc ccgccgcccg
     4381 cggaggaccc caagactttg atgagcatgg cgcttggcga gcttttcgcc ggatccggtg
     4441 caaaaattca cacccggggt caaatgcaaa cgccggcaaa tgcaaacggc ggccgaagcc
     4501 gccgtctcaa
//
LOCUS       DASTMT010000253         2272 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_464018, whole genome shotgun sequence.
ACCESSION   DASTMT010000253 DASTMT010000000
VERSION     DASTMT010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2272
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_464018"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            278..1819
                     /locus_tag="VFR00_05910"
     CDS             278..1819
                     /locus_tag="VFR00_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011313898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU0158823.1"
                     /translation="MAISQSGPAGPDLAQGIAAKDLDDGHMLAGHVGDEAVLLARSGA
                     SIFAIGAKCTHYGGPLAEGLVAGDTVRCPWHHACFSLRTGEALRAPAFMPLARWTVEE
                     RDGRIFVRAKAEPPAPRQATRATPAAASPKRIVIIGGGAAGFAAAAALRRHGYGGSIV
                     MLSGESAPPVDRPNLSKDYLAGNAPEEWLPLQPDAFYVENGIDVRLNATAAGIDVRAR
                     EVIVPDGNKLGYDRLLLATGAEPVRLSIPGANQPHVLTLRSLADCRAIIARAQGARRA
                     VVLGASFIGLEVAAALRARGVEVHVVAPEKRPMERVLGPQLGDMVRALHMEHGAIFHL
                     GEMANAIDGKRVVLKNGGVIEADLVVAGIGVRPRIELAEKAGLAIDRGLVVDAHLQTS
                     APGVFAAGDIARWPDPRSGSSIRVEHWVVAQRQGQTAARNMLGAQERFAAVPFFWSQH
                     YDVAINYVGHAESWDEVAIEGDPAGRDCLVRYKQAGRIRAVAAVHRDIASLEAEVSME
                     GQTPS"
     gene            complement(1910..>2272)
                     /locus_tag="VFR00_05915"
     CDS             complement(1910..>2272)
                     /locus_tag="VFR00_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018044671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1731 domain-containing protein"
                     /protein_id="HEU0158824.1"
                     /translation="RDDLVRLIAHAIATPELVGPLNATAPEPVQNDAFTRALGAALHR
                     PAPLRVPAGPLHALGGDFADELLLGGQRVLPAKALASGFAFRHRTLPDALAAMLGTGT
                     RAVPSAHGADRPMGVTPA"
BASE COUNT          365 a          720 c          813 g          374 t
ORIGIN      
        1 gttttccggg cgcagggtcc gagacaaagc ggcagcctgt cattgcatca tcgcttacga
       61 gggtatccat tcccgcccct atttgtgttt gtggacgcgg tcaacaaggc ccccatgctg
      121 ggctcgggtc attggatgca agccccccaa aaaggttccc gggcagggat gggcaatgcg
      181 ctgcagggaa ccttccagcc ggtgcggcat ctatgcatgg acgggccggc gaggcggcac
      241 ctggctttga gctgcatttg gtcaaaggag aagcgcgatg gcaatatcgc aatccgggcc
      301 ggctggcccc gacttggcgc agggcatcgc ggcgaaggac ctggacgatg ggcatatgct
      361 cgcgggccac gtcggcgacg aggccgttct gctggcgcgc tcaggcgctt cgatcttcgc
      421 gatcggggcc aagtgcaccc actatggcgg tccgctggcc gaggggctag tggccggaga
      481 tacggtgcgc tgtccctggc atcacgcgtg tttcagcttg cgaaccggcg aagccctgcg
      541 cgcgccggca ttcatgccgc tcgctcgctg gaccgtggag gaacgtgacg gccggatttt
      601 cgtgcgcgcc aaggcggagc cgccggctcc gcgccaggcg acgcgtgcga cgccggctgc
      661 cgcctcaccg aagcggatcg tgatcatcgg tggcggggcc gccgggtttg cggctgctgc
      721 cgccttgcgc cgacacggct acggcggcag cattgttatg ctgagcggcg agagcgcgcc
      781 gcccgtggac cggccaaacc tttcaaagga ctacttggcc ggcaacgctc ccgaagagtg
      841 gctgcccctg cagccggacg ccttctatgt cgagaacggc atcgacgtgc gactcaacgc
      901 gaccgcggca ggtatcgacg ttcgcgcacg cgaggtcatc gtccctgatg gaaacaagct
      961 cggctatgac cgcctgctgc tggcgaccgg cgccgagccg gtgcggcttt caattccggg
     1021 cgccaaccag ccacatgtat tgacattgcg atcgctggcc gattgccgcg ccatcatcgc
     1081 acgggcccag ggcgccaggc gcgccgtcgt tctgggcgcc agcttcatcg ggcttgaagt
     1141 ggccgcagcg ctgcgtgccc gcggggtcga ggtgcatgtg gtggcgccgg aaaaacggcc
     1201 gatggaacgg gtgctggggc cgcagctcgg cgacatggtg cgcgcgctgc acatggagca
     1261 cggcgcaatt tttcacctgg gcgagatggc caacgccatc gacggcaaac gtgtggttct
     1321 gaagaatggc ggcgtgatcg aggccgatct ggtggtggcc ggcatcggcg tgcgacctcg
     1381 catcgagctt gccgagaagg ctggccttgc catagaccgc ggccttgtcg tcgacgcgca
     1441 tttgcaaacc agcgcgccgg gcgtgtttgc agccggtgac atcgcgcggt ggcccgatcc
     1501 gcgcagcggc agcagcatcc gcgtcgagca ctgggtggtg gcccagcgcc agggccagac
     1561 ggccgcgcgc aacatgctcg gtgcgcaaga gcgattcgcc gcagtgccgt tcttctggag
     1621 ccagcattac gacgttgcca tcaactacgt cggtcatgct gaaagttggg acgaggtggc
     1681 catcgaaggc gatcctgccg ggagggactg cctcgtgcgc tacaagcagg cgggccgtat
     1741 tcgagcggtc gccgcggttc atcgggacat cgcgagcctc gaagccgaag tgtcgatgga
     1801 ggggcaaacc ccgtcctagg ccgtttccgg ccatgacagg ctggcgagcg ctttagagcg
     1861 gacgcaacgg gcgtggttcg ttgcgaccgc cgcgctcttg tccatccgat catgccggcg
     1921 tgacgcccat gggcctgtcc gcgccgtgtg ccgagggcac cgcgcgcgtt cccgttccga
     1981 gcatagccgc cagcgcatcc ggcagcgtgc gatggcggaa ggcaaagccg ctggcgaggg
     2041 ccttggccgg cagcacccgt tggccaccca acagcaattc gtcggcgaaa tcgccgccga
     2101 gggcgtgcag cggaccggcc ggcacccgca atggcgcagg gcggtgcagg gctgcaccga
     2161 gcgccctcgt gaatgcgtcg ttctgcacag gctcgggcgc cgtcgcgttg agcgggccga
     2221 caagttccgg ggtggcgatg gcatgggcga tgaggcggac gagatcgtcg cg
//
LOCUS       DASTMT010000254         1526 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_1122421, whole genome shotgun sequence.
ACCESSION   DASTMT010000254 DASTMT010000000
VERSION     DASTMT010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 1526)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1526)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1526
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1122421"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..730)
                     /gene="cpaB"
                     /locus_tag="VFR00_05920"
     CDS             complement(<1..730)
                     /gene="cpaB"
                     /locus_tag="VFR00_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly protein CpaB"
                     /protein_id="HEU0158825.1"
                     /translation="MKRAQFLGIAIAGVCGIGAFFGVMSLVNKPAKIVKEEVTTNTTQ
                     VLVAKTDIPLGAITGPENFRWQDWPQSALGAGYIQRSGRPNAIKDLAGSVARAPMLPG
                     EPVTPVKLVKAGEGGVLAAILPQGMRAISTRIKEETGVGRLILPNDHVDVILTRRQRG
                     RDEQDSDVIFRNVRVLAIGQLIEARDGKKLAEGNTATLELTPAQARHLAWANAVGEIS
                     LALRSIADIDSARDLATQERQPSIK"
     gene            complement(875..1378)
                     /locus_tag="VFR00_05925"
     CDS             complement(875..1378)
                     /locus_tag="VFR00_05925"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002718373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEU0158826.1"
                     /translation="MLALILLWLLPGCLAFAAAMDLLTMTIPNRIALLLLSAFLPAAL
                     LAGLGVSEIGWHLASGVSVLLIGMLMFFLGWCGGGDAKLLAAIGLWLGFDHLPDYLLF
                     TAVAGGMLATLFSLVRSVPLPGLLLTEAWAVRLHRRDAGIPYGIALAAGALLVYPHTS
                     WFASLAG"
BASE COUNT          303 a          499 c          484 g          240 t
ORIGIN      
        1 ctttgatcga cggctgcctt tcctgcgtgg cgaggtcgcg cgcgctgtcg atatcggcga
       61 tgctacgcag cgccagcgag atctcgccga cagcattggc ccacgccagg tggcgcgcct
      121 gcgccggcgt caattcgagc gtggccgtat tgccctcggc gagcttcttg ccgtcgcgcg
      181 cttcgatcag ctggccaatg gccagcaccc gcacgttgcg aaagatcacg tccgaatcct
      241 gctcatcgcg accgcgttgc cgccgggtca ggatcacgtc gacatggtcg ttgggcagaa
      301 taagcctgcc tactcccgtc tcctccttga tgcgcgtgga gatcgcgcgc atgccctgcg
      361 gtagaatggc ggccaagacg ccgccttcgc cggccttgac gagtttgacc ggcgtgaccg
      421 gctcgcccgg cagcatcggc gcgcgcgcga ccgagccggc caggtccttg atggcgttgg
      481 gacgaccgct gcgctggata tagccggcgc cgagcgcgct ttgcggccaa tcctgccagc
      541 ggaagttctc tggaccggtg attgcaccga gcggaatatc ggttttggcg accagcacct
      601 gcgtcgtgtt ggtcgtcacc tcttccttga cgatcttggc tggtttgttg acgaggctca
      661 tcaccccgaa aaacgcaccg ataccgcaca cgccagcaat ggcgatcccg aggaactgcg
      721 cccgtttcat cccgcacctt ttcgcccgac gacgagtcgt tctaaagcgg ggcccaattt
      781 gcgacacaaa cctcaacggc ccgttaattg ccggcacaat tcgagacgcc gcccaggatc
      841 gatgaggtgc agagcttgcc gctccggcgc aggctcagcc ggcgaggctc gcaaaccagg
      901 acgtgtgcgg ataaacgagc agcgcgccgg cagcaagggc gatgccatag ggaatgccgg
      961 catcacggcg atgcaagcgc accgcccagg cttccgtcag cagcaacccg ggcaggggta
     1021 ccgaccgcac cagcgaaaag agggtggcca gcatgccgcc ggcgacggcg gtaaagagca
     1081 agtagtcggg caaatgatcg aaacccagcc acaagccgat ggcggccaag agcttggcat
     1141 cgccgccgcc acaccagccc aaaaagaaca tgagcatgcc gatcagcagt accgagacgc
     1201 cgctggccag gtgccaacca atctccgaca cgccgagccc ggccagcagc gcggcgggca
     1261 gaaaggcgct caggagcaga agggcgatcc ggttcggaat ggtcatggtc aaaagatcca
     1321 tggccgcggc aaacgcgaga caccccggta gcagccagag caggataagt gcgagcacgc
     1381 ctggcaatcc ttgcagagac tttcttgctt ttcgcctgcg cccgccgcga tcagcttcgg
     1441 gcaagccagt tgctggcggc gatggcgcaa aaagagaagc cgcctgcaaa agggcaggcg
     1501 gcttcatggc cggcggagat ccgcct
//
LOCUS       DASTMT010000255         5875 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_3678093, whole genome shotgun sequence.
ACCESSION   DASTMT010000255 DASTMT010000000
VERSION     DASTMT010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 5875)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5875)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5875
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3678093"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..461)
                     /locus_tag="VFR00_05930"
     CDS             complement(<1..461)
                     /locus_tag="VFR00_05930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0158827.1"
                     /translation="MGRSVSVAVAGVFAAQRSWRLNAWRAFLALGFVAGVVLVAFPEL
                     DLRVAEATFSPATGFIGGRHGWWGVVRSAFIVFYFTALAISIVGWLASRKGSLPPFGA
                     KEWLFMLVCLSVGPGLLANVGFKDQWGRARPKHIEAFGGTRVFTPALLPTN"
     gene            721..1863
                     /locus_tag="VFR00_05935"
     CDS             721..1863
                     /locus_tag="VFR00_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007602465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU0158828.1"
                     /translation="MTTAELPRADLAVTPPGVWAPPELSIVVPTLNENGNVPLLVLQL
                     GRALKGIRWELIFVDDDSNDGTIATARGLARRDPRIRCLRRIGRRGLAGALIEGMLAA
                     SAPAIAVMDADLQHDEKCLPLMLEQFRAGADLVVGTRRTQDGLVTAGLSPLRKWGSRL
                     ATALARRLARVELADPMSGFFLIRQEAFEAMAPRLSRQGFKLLLDIVASQPTPLLVKE
                     VAYSFRPRRHGHSKLDGLVAFDYAGLLLAKASGDLVSTRFLLFMLVGASGLGVHLLAL
                     KGALGLAQLAFDLAQTWAATIAMTWNFFLNNWLTYRDRRLRGPALLKGLLSFYAVCSV
                     GAVANVGVASWIYGGEPTWWLAGGAGALMGGFFNYAASSTFTWRQR"
     gene            1904..3400
                     /locus_tag="VFR00_05940"
     CDS             1904..3400
                     /locus_tag="VFR00_05940"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEU0158829.1"
                     /translation="MLLLAGAIVLLALLRALVAATTELSDDEAYYRLWALAPAMSYLD
                     HPPMVAWLIAAGQAVLGDTPLGVRLAAVLTSLVGPLLLWRTVWLLFGAEVAERAVAVA
                     LAMPLLAVGGIIITPDLPSVLFWGLTGWAMAELYVSDNAWWWLAVGVFAGLGLLSKYT
                     NLFVGIGILIWLIGLKPNRRWFGTWQLWAGGALSLLLTGPVIVWNSAHGWASLGKQFG
                     RVVRTEAFTPAYLLEAVGSFVLLASPIIAGLAAIGVGALITAVARRRDPGAFLLLASL
                     LPLLLYFAVHAIHARVQPNWMAPLYPVLAAAAAIAATARARGIAAARTWVSATGAALA
                     LGLLLSGLILAHAVSPLMVNAKDPTSQMRGWRAFAGDLEALRRRHGACAVATSHYTIS
                     AQLAYHLPPGVPVLPLNEPLRYVHLPPVTVPDCDMLYVELERRQRLAGLEASFRSIEP
                     LGTVTRSTAGHVLARYTVHLLRGPHAPAAMPDLRRRHWRASIVAAGST"
     gene            3489..5099
                     /locus_tag="VFR00_05945"
     CDS             3489..5099
                     /locus_tag="VFR00_05945"
                     /inference="COORDINATES: protein motif:HMM:NF020976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HEU0158830.1"
                     /translation="MTRMRLRGPSVPPGQQGPTRRRVLAASGAFAITLLTGQRAGAKR
                     GNPFSLGVAAGEPTPDGFVLWTRLAVEPLSADGLGGMREPVPVTWEVARDQTMRDVVR
                     QGTVEANPASAHSLHVEVGGLEANRPYWYRFTSQGEHSSIGRARTTPAPGDRLERLRF
                     AFASCSNWQLGYFSAYRHMAEENPDLVIFLGDYIYEYTLSAASQGRIVRPHDGPTATN
                     LAGYRNRYALYRTDPDLQALHAAAPCLVTWDDHEVENDYANQWSQDMRVEPEAFLARR
                     AAAYQAYYEHMPLRPRPSGAPQSFRLYGRTAFGDLVSFSLLDGRQYRSKQPCEVPPSR
                     RGHVAPLSCSERLDARRTLLGAAQEEWLFEGFKRAETAWNVLAQGQLMAELRQKSFAG
                     EVGFWTDSWDGYPVARQRLLEAVSTSRLANPVVIGGDIHSFWTTDLKADFKDPRSATV
                     ATEFVGTSITSDPPPHGLIAGLLPDNPHVRYFESRHRGYVLVDVDRERMLVNFRSISD
                     RRDPKATLATLKQFAVVSGRAGAIDING"
     gene            complement(5189..>5875)
                     /locus_tag="VFR00_05950"
     CDS             complement(5189..>5875)
                     /locus_tag="VFR00_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011650105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HEU0158831.1"
                     /translation="RASYRLAYRDARSVTDIAMDAGYDAPDAFARAFRQRFGQSPSSF
                     RKSPDWEPWLAAFEPLDNARSKLMQKTFTADDVTIRDVPPTPVAIMEHRGDPATLGAT
                     IQRFIAWRQAAGLHPKTNPTFNVWRSERRPASPADYSVDLCVGTDQPIEPNGEQVKAG
                     EIPGGRCAVLRVVGFIHNLEPAALYLYRDWLPASGEQARDFPIYCQRLSFFPEVPEHE
                     AVAELFLPLQ"
BASE COUNT          885 a         1975 c         1978 g         1037 t
ORIGIN      
        1 tggttggtcg gcagcagcgc cggggtgaaa actcgcgtgc cgccgaaggc ctcgatgtgc
       61 ttgggtcgtg cccggcccca ctgatccttg aaaccgacgt tggccagcaa gcccggcccg
      121 acgctgagac acacgagcat gaacagccac tccttggcgc cgaatggagg aagcgagccc
      181 ttgcggctgg cgagccagcc cacaatcgag atggcaaggg cggtgaaata aaagacgatg
      241 aaggccgaac gcaccacgcc ccaccagccg tgcctcccgc cgatgaagcc ggttgccggc
      301 gagaaggttg cctcggcgac gcgcaggtca agctccggga aagcaacgag caccacaccg
      361 gcgacaaagc ctagcgcgag gaaagcgcgc caggcgttga gccgccaact gcgctgcgca
      421 gcgaaaacgc ctgctaccgc cacggacact gatcgcccca ttcgttcgcc ttgatccccg
      481 gcggcagggt gggcgtggga tgtgaaggcc gtgttggagt ttcaggaagg tttcgtgaca
      541 gcgccggctt tgttgcgccc gcgccgcagc cgcgaggttt gcccatttgg cgggcatcgg
      601 cgcgggtgcc acagcgcgcc gaccgtcatc gttccgtcat cgagctttca tgcttccgca
      661 atggtcgctt gcgagcgtgc gcggcgcttg gctggggaca tacgaagcga cgggggctgg
      721 atgacaacgg cagagctgcc gcgcgccgac ctggcggtca cgccgcccgg ggtgtgggcg
      781 ccgcccgagc tttccattgt cgtgccgacg ctgaacgaga acggcaacgt cccgctcctc
      841 gtgttgcagc ttggccgcgc gctcaaaggc attcgctggg agctcatctt tgtcgacgac
      901 gacagtaacg atggcaccat cgccaccgcg cgcggcctcg cccgccgcga cccgcgtatc
      961 cgctgcctgc gccggatcgg ccgccgcggc ctggcgggcg ccttgatcga gggcatgctg
     1021 gcggcctcgg cccccgccat cgccgtcatg gatgcggact tgcagcacga cgagaagtgc
     1081 ctgcctctga tgctcgagca gtttcgcgcc ggcgccgacc ttgtggtcgg gacgcgacgc
     1141 acccaagacg gcctggtgac ggcggggctc tctcccctgc gcaagtgggg cagtcgccta
     1201 gccacggcgc tggcgcggcg gctggcgcgg gtcgagcttg cggatcccat gagcgggttc
     1261 tttctgatcc gccaggaggc cttcgaggcg atggcaccgc ggctatcgcg gcaaggcttc
     1321 aagctgctgt tggatatcgt tgcctcgcag ccgaccccgc tcctggtcaa agaggtcgcc
     1381 tacagctttc gcccccgccg gcacggccac tccaaactcg acggcctggt ggccttcgac
     1441 tacgccggtc tgctgttggc caaggcgtcc ggcgacctcg tctcgacgcg atttttgctg
     1501 ttcatgctgg tcggggccag cggcctcggg gtgcatctgc tggcgctgaa aggcgcgctc
     1561 ggcctggcgc agctcgcatt cgacctcgcc caaacctggg ccgccacgat cgccatgacc
     1621 tggaatttct tcctcaacaa ttggctgacc tatcgcgacc gccggctgcg tggtcccgcg
     1681 ctgctcaaag gtctgctgtc gttttatgcc gtgtgcagcg tcggtgcggt cgccaacgtc
     1741 ggtgtggcga gctggattta tggcggggag ccgacctggt ggttggcggg tggggcgggt
     1801 gcgctgatgg gagggttttt caactacgcc gcaagttcta ctttcacctg gcgtcagcgc
     1861 tgattcgccg ccgagcgagc gccgagccgc gctgtcgacg ccgatgctcc tgctcgcggg
     1921 ggccatcgtg ctgctggcgc tgctgcgcgc cctcgtggca gcgacgaccg agctcagcga
     1981 tgatgaggcc tactatcggc tttgggcgct ggcgcccgct atgtcctacc tcgaccaccc
     2041 accgatggtg gcgtggctga tcgcggcggg ccaggccgtc ctgggcgaca cgcccttggg
     2101 cgtcaggctg gccgcggtgc tgacgagcct cgtcgggccg ctgctgctgt ggcgcacggt
     2161 gtggcttctg ttcggagccg aggtggccga gcgcgccgtc gccgtcgcgc tcgccatgcc
     2221 cttgctcgcg gtcggcggca ttatcatcac gccggatctg ccctctgtcc tgttttgggg
     2281 gctaacggga tgggcgatgg cggagcttta cgtttccgac aatgcctggt ggtggctggc
     2341 cgttggcgtg ttcgcgggtc ttggcctcct ctccaaatac accaaccttt ttgtcggcat
     2401 cggtattctg atctggctca tcggcctcaa gccaaaccgg cgctggtttg gcacttggca
     2461 actgtgggcc ggcggcgcgc tgagcctgct gctcaccggg ccggtcatcg tctggaacag
     2521 cgcgcatggc tgggcatccc tcggcaagca attcgggcgt gtggtccgca ccgaggcctt
     2581 cacccctgcc tatctgttgg aagccgtggg cagcttcgtg cttttggcca gccccatcat
     2641 cgccggcctg gccgccatcg gcgtgggagc tctcatcacc gcggtcgcga gacgccgcga
     2701 cccgggggcc ttcttgctcc tcgccagcct gctgccgctc ctcctctatt ttgctgtgca
     2761 cgccatccac gcgcgggtac agccgaactg gatggcgccg ctctaccccg tgctggcggc
     2821 tgcagcggcg atcgccgcca cggcacgagc ccgcggcatc gccgcggcgc ggacatgggt
     2881 tagcgccacc ggcgccgcgt tggcgctcgg cctgttgctg tccggcctga tcttggcgca
     2941 tgccgtctcg ccgctgatgg tcaatgcgaa ggatcccacc agtcagatgc ggggctggcg
     3001 agcgtttgcc ggggacctcg aggctttgcg caggaggcat ggggcctgcg cggtggcaac
     3061 gtcgcactac accatcagcg cgcagctcgc ctaccacctg ccccctggtg tacctgtcct
     3121 gcccctcaac gaacccttgc gctacgtcca cctacccccg gtaaccgtgc cggactgcga
     3181 catgctctac gtcgagctcg agcgccggca acggcttgcg gggctcgagg caagctttcg
     3241 ttcgatcgag ccgctgggga cggtgacgcg cagcactgcg ggccacgtcc tcgcccgcta
     3301 cacggtgcac cttctgcggg gtccccacgc acccgcggcg atgccagatt tgcggcgccg
     3361 acactggcga gcgagcattg tggccgccgg cagcacctga ggagggttgc cgtaatttaa
     3421 gtactcgctg gcgtcgtcct cgcctacaag cctcttaagc cggcgcatgt tgccaaggag
     3481 atgagccgat gacgcgcatg cgcttgcgtg gccccagcgt gccgccgggg caacagggcc
     3541 cgacgcgacg gcgcgtcctg gctgcctccg gcgcctttgc catcacgctt ctcaccggcc
     3601 agcgcgcggg cgccaagcgc ggcaatccct tcagcttggg ggtggcggcg ggcgagccga
     3661 cgcccgacgg ctttgtgctc tggacgcgcc tggccgtcga gcccctgagc gccgatggcc
     3721 tgggcggcat gcgcgagccg gtgcccgtga cctgggaggt tgcgcgcgat cagacgatgc
     3781 gtgacgtcgt gcgccaaggc acggtcgagg ccaacccggc atcggcgcat tccctgcacg
     3841 tcgaggtggg cggtctcgaa gccaatcgtc cttactggta ccgctttacc agccagggcg
     3901 agcacagctc gattgggcgc gcgcgcacga ccccggcgcc gggcgaccgg ctcgagcgct
     3961 tgcggtttgc cttcgcctcc tgctccaact ggcagttggg gtatttcagt gcctatcggc
     4021 acatggccga ggagaatccc gatctcgtca ttttcctcgg cgactacatc tacgagtaca
     4081 ccctctcggc ggcctcgcaa ggtcgcatcg tccggccgca cgacggtccc accgccacca
     4141 atcttgccgg ctatcgcaat cgctatgcgc tctaccgcac ggacccggac ctgcaagcgc
     4201 tgcacgccgc cgcgccctgt cttgtcacct gggacgacca tgaggtggaa aacgactacg
     4261 ccaatcaatg gtcgcaggat atgcgcgtcg agcccgaggc gtttttggcg cggcgggcgg
     4321 cggcctatca ggcctattac gagcacatgc ccctgcgccc ccggcccagc ggcgcgccgc
     4381 agtccttccg cctctatggg cggacggcct tcggcgatct cgtgagcttc tcgctgctgg
     4441 atggccggca gtatcgctcc aaacaaccct gcgaggtgcc gccctcgcgg cgcggtcacg
     4501 tggcgccgct gagctgctcg gagcggcttg acgcgaggcg gacgctgctt ggggcggcgc
     4561 aggaggaatg gctgttcgag ggcttcaaac gggccgagac cgcgtggaac gtcctggccc
     4621 agggtcagtt gatggccgag ctcaggcaga agagctttgc tggcgaggtc ggcttttgga
     4681 ccgacagctg ggacggctat cccgtggcgc gccaacgcct gttggaggcg gtgagcacga
     4741 gccggctggc gaaccccgtc gtgatcggcg gcgacatcca ctcgttctgg acgaccgacc
     4801 tcaaggccga cttcaaggat ccgcgctcgg caacggtcgc cacggagttc gtgggcacct
     4861 ccatcacctc cgatcccccg ccgcacggcc tgatcgccgg cctgctaccg gataatccgc
     4921 atgtgcgcta tttcgaaagc cgccaccgcg gctatgtgct ggtcgatgtc gatcgcgagc
     4981 gcatgctcgt caacttccgc agcatctccg accgccgcga tcccaaggcg acgctggcaa
     5041 cgctcaagca gttcgcggtc gtgagcggcc gagccggcgc catcgacatc aacgggtaag
     5101 ccgttaatcc ataaaaggcc gagatcgggt gaatagcggc ctgtccgcgg ctcgcgatcg
     5161 agaagggacg ggccgctgac gcagcgcgct attgcagcgg caaaaatagt tcggcgaccg
     5221 cctcatgctc cggcacctcc gggaagaagc tcagccgctg gcaatagatc gggaagtcgc
     5281 gtgcttgctc gccgctggcc ggaagccagt cgcgataaag gtagagcgcg gcgggctcga
     5341 gattgtggat gaagccgacg acgcgcagca ccgcgcagcg tccgccagga atctcgccgg
     5401 ctttgacttg ctcgccattc ggctcgatcg gttggtcggt cccgacacaa aggtccacgc
     5461 tataatcggc aggcgacgca ggacgccgct cggaacgcca gacattgaag gtcgggttgg
     5521 tcttggggtg caggccagcg gcctggcgcc aggcgatgaa ccgctggatg gtggcgccga
     5581 gcgtcgctgg gtcgccgcga tgctccatga tcgccaccgg cgtggggggc acatcgcgaa
     5641 tcgtcacgtc gtcggcagta aacgtcttct gcatgagctt gctcctcgcg ttgtcgagag
     5701 gctcgaaggc cgcaagccac ggctcccagt cgggagattt ccggaacgac gaaggcgatt
     5761 gcccgaaccg ctgccgaaag gcgcgggcga aggcgtccgg tgcatcgtaa ccggcatcca
     5821 tcgctatgtc cgtgacgctt cgggcatccc tgtaggcgag ccggtatgaa gcgcg
//
LOCUS       DASTMT010000256         5412 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_5648633, whole genome shotgun sequence.
ACCESSION   DASTMT010000256 DASTMT010000000
VERSION     DASTMT010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 5412)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5412)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5412
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5648633"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            99..623
                     /locus_tag="VFR00_05955"
     CDS             99..623
                     /locus_tag="VFR00_05955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011501733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU0158832.1"
                     /translation="MRVASRRDAVAAEIPRLRRYARALVGDSSEADDLIQDTLERALA
                     RLEQWRDGDNPRKWLFSILHNLHVDGLRQKSRRPPHVGLDSLGPDQSAPAADGASGSD
                     LDRALQQLTADQREVVLLVGLEGLSYAETAEVLSIPMGTVMSRLARGRDRLRGLLALG
                     LENEAEPAGLRRVK"
     gene            620..1405
                     /locus_tag="VFR00_05960"
     CDS             620..1405
                     /locus_tag="VFR00_05960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HEU0158833.1"
                     /translation="MTGARRISEAELHAFVDGELASKERAEVEALLAAAPEGLRTVRG
                     LTELNERLARHYVHVLEEPIPARLAALTAKPRVRARLSPAWARAAALVGLLVAAAAAG
                     YLVRDFRGGNGGPQPAFVASALSAHQVFVPEVRHPVEVGAGEEKHLVQWLTRRLGTEV
                     RAPELGAEGWKLMGGRLLPDRGQAAAQFMYEDVSGRRLTLYVRKEAGLTDAAFRFAER
                     GDFGAFYWIDRSLAYALAGRLDRGELMRLATSIHAQLGGVGSG"
     gene            complement(1439..1732)
                     /locus_tag="VFR00_05965"
     CDS             complement(1439..1732)
                     /locus_tag="VFR00_05965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0158834.1"
                     /translation="MTVIPFRDRRVARPPRRPVEPPSALKNQQTSAAAAEEDAEFYRV
                     RARQNWAVLALIVALVVGGAWLIERLAAFSRAVACYEFGHRNCMPLKIDLPPR"
     gene            complement(1958..3310)
                     /gene="glmM"
                     /locus_tag="VFR00_05970"
     CDS             complement(1958..3310)
                     /gene="glmM"
                     /locus_tag="VFR00_05970"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEU0158835.1"
                     /translation="MTERYFGTDGIRGQANRPPMTSEIALKVGMAAGKFIRDGSHRHR
                     VVIGKDTRLSGYMIESALMSGFTAVGMDVFLLGPMPTPAVAMLTRSLRADLGVMISAS
                     HNPFDDNGVKIFDRDGYKLSDEAERAIEKLMNMPSADLLVAPERIGRAKRIDSAQERY
                     VEYAKRTLPRNISLTGLRVVVDCANGAGYEVSPDALWELGAEVITIGVEPNGLNINLK
                     CGSTSPEALGEKVREMRADIGIALDGDADRVLITDEKGRIVDGDQIMCVIAESWHKSG
                     RLNGGGVVATVMSNLGMERYLKGLGLTLERTPVGDRYVVDYMRKHGYNIGGEQSGHIV
                     LSDFGTTGDGLVAALQVLAVVVATGKPVGEVCTRFQRLPQILENVKYANGSPLEHADV
                     VKAIASGKERLGAQGRLVIRPSGTERVIRVMGEGDNEPLLQQVIGDISAAVRKAAEVA
                     "
     gene            complement(3729..4793)
                     /gene="folP"
                     /locus_tag="VFR00_05975"
     CDS             complement(3729..4793)
                     /gene="folP"
                     /locus_tag="VFR00_05975"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR01496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEU0158836.1"
                     /translation="MHDTLAIRAIALIGRATAGAADRAQLRVAGRTQPSFACAELMRR
                     NRDGTTRRLLSAAELRADADPRLVELLAELERPRAAIAGLGFDRPRLMGIVNVTPDSF
                     SDGGRHYDVAAAVEHGLRLEAEGADLLDIGGESTRPGAAGLTLEEECRRVLPVIDRLV
                     REARVPLSIDTRNSEVMRRAAASGVRLINDVSALRHDPNSLSCVAETSLPVVLMHAQG
                     DPATMQDAPHYADVVLDIYDFFTERIAACEAAGIARSRLILDPGIGFGKTLTHNLELL
                     RALSVFHGLGCVLMLGASRKSFIAHLSGAAPLDRLPGSLAAALIGTAQGVQLLRVHDV
                     AETRQALAVWQGATSGPAGA"
     gene            complement(4841..>5412)
                     /locus_tag="VFR00_05980"
     CDS             complement(4841..>5412)
                     /locus_tag="VFR00_05980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HEU0158837.1"
                     /translation="NDNRITQLTIDGSKVEVAKREIAAGLRPYGLDISAKGEVAVVAN
                     IGVGGGDADTISVIDLKLDPPRVVGTYTVGQTPEGIKLSPDGKFVAVTVMNGSNKAAN
                     SPFFNGNGFLQIWTRTGTQLTKTSELPIGRWCQGIAWSANGKTLLVQCMVEEEINVIR
                     FSGLAGRSLQKVGSIKTKGGPAGIRTAEP"
BASE COUNT          856 a         1745 c         1858 g          953 t
ORIGIN      
        1 gcgccgcaca ttattttgat gcgtcgcgcg gaataaattg gaggccaccg tcgtctgctg
       61 gcataagtcc tactgtgtgc cggccagtag gaaaggacgt gcgcgtggcc tctcgccggg
      121 atgccgttgc ggcagaaatt ccacgcttgc ggcgttacgc gcgggccctg gtcggcgaca
      181 gcagtgaggc ggatgatctg atccaggaca cgctggagcg tgctctggca cgcctggagc
      241 aatggcggga tggagacaat ccacgcaagt ggttgttttc tatactgcat aatctgcacg
      301 tggatggctt gcggcaaaaa tcccgccgcc ccccacatgt cggtctcgac agcctcggcc
      361 cggaccagtc ggcaccggcg gccgacgggg cgagcggctc ggatctcgat cgggcgctgc
      421 aacagctgac ggccgatcag cgggaggtgg tgctgctggt cggccttgaa ggtttgagtt
      481 acgcggagac ggctgaggtg ctgtcgatcc ccatgggaac cgtgatgtcc cgcctggcgc
      541 gcgggcgcga tcggctgcgc gggctcttgg cccttgggct ggaaaacgag gccgagcccg
      601 cggggttgcg gagggtcaag tgaccggtgc gcgacgcatc agcgaggcgg agctgcacgc
      661 ctttgtggac ggcgaactcg cgagcaagga gcgcgccgag gtcgaggccc ttttggccgc
      721 ggccccggag ggtttgcgca cggtgcgtgg gctcacggag ctgaacgagc gcctggctcg
      781 gcactacgtc catgttctcg aggagccgat ccccgccagg ctggcggcgt tgacggccaa
      841 gccacgcgtg cgtgcacgcc tctcgccggc atgggcgcgg gccgcggcac tggtgggcct
      901 cctcgttgcc gcggcggcgg ccggctacct ggtgcgggat ttcagaggcg gcaacggcgg
      961 cccgcaaccc gcgttcgtgg cgagcgcgct cagcgcgcat caagtgttcg tgccggaggt
     1021 gcgccatccc gtcgaggtgg gggccggcga ggaaaaacac ctggtgcagt ggctgacgcg
     1081 ccggctcggc accgaggtgc gtgcccccga actcggcgcc gagggctgga aattgatggg
     1141 gggacggctg ctgcccgacc gcggccaggc cgcggcgcaa ttcatgtacg aggatgttag
     1201 cggccggcgc ttgaccctct atgtacgcaa ggaggcgggt ctcacggatg ccgctttccg
     1261 cttcgccgag cgcggcgatt tcggcgcctt ttattggatc gaccgctcgc tcgcctatgc
     1321 gcttgcgggt cgcctcgatc gcggcgagct gatgcggctg gcgacgagca ttcatgctca
     1381 gctgggcggg gtgggttcgg gctagctgtg cggggggctg ccggcccggc agcggcgatc
     1441 agcgcggcgg caggtcgatt ttgagcggca tgcaattgcg gtgcccgaac tcgtagcagg
     1501 ccacggcgcg ggagaaggcc gccaaccgct cgatcagcca ggcgccgccc accaccaagg
     1561 cgacgatgag cgcgagcacg gcccaattct gccgggcgcg cacgcggtag aactcggcgt
     1621 cttcctccgc ggcggcggcc gacgtttgct ggtttttgag ggcagacggc ggctcgacgg
     1681 gccgcctcgg cgggcgggct acgcgtcggt cgcgaaaggg aatgaccgtc atgttttttc
     1741 ggcgcgctct ctcggcgctg atgaggtgcg gcgccgcccg cccgggccga ccctgcaagg
     1801 gcggccgagg ccaagcggcg ttcgcggccg cagcaatctt gccagagccg aagaggccgt
     1861 caagccggct tgttgcgcgc ctggatccgc cggccagccc cggcaccggc ttggtgatgc
     1921 cgtgcccaaa ggcgcaactg gcgcagggcg tttcgtgtta agcgacctcg gctgccttgc
     1981 gcaccgcggc ggagatgtcg ccgatcacct gctgcagcag aggctcgttg tcgccctcgc
     2041 ccatcaccct gatgacgcgc tcggtgccgg agggacggat aacgaggcgg ccctgcgcgc
     2101 ccaggcgctc cttgcccgag gcgatggctt tcacgacgtc ggcgtgctcg agcggcgagc
     2161 cgttggcgta tttgacgttt tcgaggattt gcggcaggcg ctggaagcgc gtgcacacct
     2221 cgcccacggg cttgccggtg gcaacgacca cggccagcac ctgcagggcc gccaccagac
     2281 cgtccccggt ggtgccaaag tccgacagca caatgtgtcc cgattgttcg ccgccgatgt
     2341 tgtagccatg cttgcgcatg tagtcgacga catagcggtc gccgaccggc gtgcgctcca
     2401 acgtcaggcc gagacccttg aggtagcgct ccatgccgag gttcgacatc acggtggcga
     2461 ccacgccgcc gccgttcagg cgcccgctct tgtgccagct ttcggcaatc acgcacatga
     2521 tctggtcgcc atcgacgatg cggccttttt cgtcggtgat gaggacgcga tcggcatccc
     2581 cgtccagcgc gatgccgata tcggcgcgca tttcgcgaac cttttcgccc agcgcctccg
     2641 gcgaggtgga cccgcacttc agattgatgt tgagcccgtt cggttcgacc ccgatggtga
     2701 tcacctccgc gcccagttcc cacagggcat cgggtgacac ttcgtagccc gcaccattgg
     2761 cgcagtcgac gacgacgcgt agccccgtca ggctaatatt gcggggcaac gtccgcttgg
     2821 catactcgac gtagcgctcc tgcgcactat cgatgcgttt ggcgcgacca atgcgctcgg
     2881 gtgcgaccag caagtcggcg ctcggcatgt tcattagctt ctcgatcgca cgctcggcct
     2941 cgtcggagag cttgtagccg tcgcggtcga agatcttcac cccgttgtcg tcgaagggat
     3001 tgtgcgacgc cgaaatcatg acgccgagat ccgcgcgcag ggagcgggtc agcatggcaa
     3061 cggccggcgt cggcatcgga cccagcaaaa agacgtccat gccgaccgcg gtgaagcccg
     3121 acatgagcgc cgattcgatc atgtagccgg agaggcgcgt gtccttgccg atcacgacgc
     3181 gatgccgatg gctgccatcc ctaatgaatt tacccgccgc catcccgacc ttgagggcga
     3241 tctccgaggt catgggcggc cggttcgcct ggccgcggat gccatccgtt ccgaaatagc
     3301 gctctgtcat ggctttcgcc ttttgcctcc tggctgccgg cttgggcctt ctggcttctg
     3361 cctcatgcct tggtgcaagt gccttcgctg caagtccctt tgctacgcgt gcctttgtgc
     3421 aaacttggta tatgcgaact tggtgtgtgc aaacttggtc gtgcaaactt ggtgcatgcg
     3481 gacttgatgc atgcgagcct ggtgcatgca accttggtgc atgcgaactt agtgcatgca
     3541 aacttgatgc atgcaaactt gatgcatgca aacttggtgc aagtcccggg ccagtgcggt
     3601 ccggtcgcga tcgcgctgcc tccgccgggc agccgtcggg acggtcgaga ctacaccaat
     3661 gtgccagcaa cttgcgagac ggacagcggc ctttcaacag tcccggcgcg tgcccacgca
     3721 cgcaggcgct aagcgcctgc gggcccggag gttgcgccct gccagaccgc gagcgcctgg
     3781 cgggtctcgg ccacatcgtg cacgcgcagc agctgcacac cttgagctgt gccgatcagc
     3841 gcggcagcca gcgacccggg caagcggtca agcggcgctg ccccgctcag gtgcgcaatg
     3901 aagcttttgc gcgaagcccc cagcatgagg acgcagccca agccatggaa gacgctgagc
     3961 gcgcgcaaaa gctcgagatt gtgcgtcaag gttttgccga agccgatgcc ggggtcgagg
     4021 atcagacggc tgcgcgcaat gcccgcggcc tcgcaggccg cgatccgttc ggtaaaaaaa
     4081 tcgtaaatat cgagcaccac gtcggcgtag tgcggcgcgt cctgcatggt ggcgggatcg
     4141 ccctgcgcat gcatcaggac aaccggcagg ctggtctcgg ccacgcagct caggctgttg
     4201 ggatcgtgcc gcaaggccga gacgtcgttg atgagccgca cgccgctggc cgccgcgcgc
     4261 cgcatgacct cgctgttgcg cgtgtcgatt gagagcggca cgcgggcctc tcgcaccagc
     4321 ctgtcgatca cgggcaagac acggcggcac tcctcctcca acgtcagacc cgcggcgccg
     4381 ggtcgcgtcg attccccacc aatgtcgagg aggtcggcgc cctcggcctc caggcgcagg
     4441 ccatgctcga ccgccgcggc cacgtcgtag tgccgaccgc catcggaaaa gctgtcgggt
     4501 gtcacattaa cgatgcccat gaggcgcggc cgatcgaagc cgaggccggc gatggccgcg
     4561 cgcggtcgct cgagctccgc cagtagctcg acgagccgag gatcggcgtc ggcgcgcaac
     4621 tcggcggccg acaagagccg acgcgtcgtg ccgtcgcgat tgcgtcgcat cagttcggcg
     4681 caggcaaacg acggctgagt gcggccggcc acgcgcagct gggcgcgatc ggcagcgccg
     4741 gccgtcgccc tgcctatgag ggcgatagcg cgaatggcga gggtgtcgtg cacttagcgg
     4801 tgcttacgcc agcggcggcg tgctggctag ccccgcgcgt ctagggttcc gccgtgcgga
     4861 tgccggccgg accacctttg gtctttattg agcccacctt ctgcagcgag cggccggcga
     4921 gacccgagaa gcggatgacg ttgatctcct cctcgaccat gcattgcacc aacagcgtct
     4981 tgccgttggc ggaccaggcg atgccctggc accagcgccc gatgggcaac tcggaggtct
     5041 tggtcaactg cgtgccggtg cgcgtccaga tctgcaggaa gccgttgccg ttgaagaacg
     5101 gagagttggc ggccttgttg gagccgttca tgacggtgac ggcgacgaat tttccgtccg
     5161 gcgaaagttt gatgccctcc ggggtctggc cgacggtgta ggttccaacc acgcgcggtg
     5221 gatcgagctt gagatcgatg acactgatgg tgtcggcatc gccgccgccg accccgatgt
     5281 tggccaccac ggccacctcg cccttcgcac taatatcgag cccgtagggt cgcaagcccg
     5341 cggcaatttc gcgcttggcc acttccacct tgctgccgtc gatggtcagc tgggtgatgc
     5401 gattgtcgtt gt
//
LOCUS       DASTMT010000257         6243 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_1775605, whole genome shotgun sequence.
ACCESSION   DASTMT010000257 DASTMT010000000
VERSION     DASTMT010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 6243)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6243)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6243
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1775605"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..583
                     /locus_tag="VFR00_05985"
     CDS             <1..583
                     /locus_tag="VFR00_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010969150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HEU0158838.1"
                     /translation="AFAAIGQRNIKRLLAYSAIGNIGYMLIGLAAASPEGARGVIIYL
                     VIYVAMTLGAFACVLSMRRAEGMVEDIDELAGLAQSNLGMATVFGLLMFSLAGVPPLA
                     GFFAKFYVFVAAVKEGLWPLAIFGVLASVVGAYYYVRIVKVMFFDAPKEKFLAVPAKA
                     GLIMALTGLFVLLYVVWPAPLVGSADAAAKSLF"
     gene            613..1365
                     /locus_tag="VFR00_05990"
     CDS             613..1365
                     /locus_tag="VFR00_05990"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HEU0158839.1"
                     /translation="MPHAIRSIVLDQVGSTNAEAFAQAMAGELGPLWIMAHRQTQGRG
                     RSGRRWVSEPGNLHASLLLHLACPPAAVHQLSLLAGVAVIDAVTTAAGRPISGLRLKW
                     PNDVLVGEAKCAGILPESHAGAAAAQPLAVIGVGINLAWHPQDLGRPVTHLAAHGVVV
                     DPEAMLAHLAGAMQRWLDVWDWGIGFAAIREAWSARGGPAGEALSVDTGREKISGQYL
                     DLAEDGALIMLDSNGRERRLTYGEVTIGRRGI"
     gene            1463..3088
                     /locus_tag="VFR00_05995"
     CDS             1463..3088
                     /locus_tag="VFR00_05995"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEU0158840.1"
                     /translation="MNCYLYGLGPAEAREWLMVDLGITFPEGENDPGIDVILPDLRFI
                     EEERAALAGIVLTHAHEDHFGAVAELWSRLKVPVYATPFAAALLTAKLAEFGGRERPE
                     VRVKPLDSRFQIGGFDIELLSLAHSIPESNALVIRTPLGCVLHTGDWKLDPTPLIGAP
                     AELGRLAQLGDDGITAIVCDSTNAMREGRSPSERDVARSLAGLVRAATGRVAVTIFAS
                     NVARIRAVADAARSAGRHLVVAGRAMHRIIDAAMETGYLPSDFKYYDQEHYQYLERDE
                     TVVLCTGSQGEPRAAMARIAEGQHPHISLGKGDLVIFSSRTIPGNERAVGRIHNQLID
                     LGCELVTDADALVHVTGHPRREELKEMYAWVRPRVAIPMHGEARHLAEHAKLARAAGV
                     KEVVGVRNGDIVRLAPGAAAVIDEAPIGRIFRDGRLLVSADEGPVRQRRALAFAGIVI
                     VAITLSGRGELLAEPVITLDGVPAFDAAGQSVLEHLLDAVEATLKSIPPPRRKDGETV
                     REAVRRAVRARVEDLWGKRPITKVLLTQLGMRG"
     gene            3118..3522
                     /gene="mce"
                     /locus_tag="VFR00_06000"
     CDS             3118..3522
                     /gene="mce"
                     /locus_tag="VFR00_06000"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003608863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HEU0158841.1"
                     /translation="MIGRLNHVAIAVPKLAEAATLYANALGANVSQPVPHPEHGVTVV
                     FVTLPNTKIELLEPLGERSPIAAFLERNPSGGIHHVCYEVADLAAASAQLKAAGARVL
                     GDGEPKIGAHGKPVLFLHPKDFNGTLIEIEQA"
     gene            3530..3796
                     /locus_tag="VFR00_06005"
     CDS             3530..3796
                     /locus_tag="VFR00_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1467 family protein"
                     /protein_id="HEU0158842.1"
                     /translation="MTPTLAIAIYVLLWWVVLFAVLPLGVRTQGEDGTVVPGTPESAP
                     TAPRFLRILLLTTLVTSLLFGAFWATIRYDLIDYAYWLGGATPP"
     gene            4264..5610
                     /locus_tag="VFR00_06010"
     CDS             4264..5610
                     /locus_tag="VFR00_06010"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEU0158843.1"
                     /translation="MRLSRFFLPVLRETPKEAEIVSHQLMLRAGMVRQASAGIYSWLP
                     LGLKVLQRIETIVREEQDRSGAIEMLMPTLQQADLWRESGRYDAYGPEMLRFRDRHER
                     EMLYGPTNEELITQIFRDAVRSYRDLPRNLYHIQWKFRDEVRPRFGVMRGREFLMKDA
                     YSFDISAAAARQAYHRMFVAYLRTFARLGLKSIPMAADTGPIGGDLSHEFIILAETGE
                     SRVYCHADLLDLRVPPADVDFAGDLAPLIEQWTKPYAATDEKHEQARFAAEVPPQKRV
                     SARGIEVGHIFFFGTKYSEPMGCRVQGPDGSMIAVQMGSYGIGVSRLVGAIIEASHDA
                     AGIVWPVPVAPFEVALINLKAGDKDTDRACEDLYARLMRAGVSVLYEDTEERAGAKFA
                     SMDLIGLPFQLIVGPKGVKNGEVEVKERRSGERVNLAPDAAVKRLVELISARRLLV"
     gene            5650..>6243
                     /locus_tag="VFR00_06015"
     CDS             5650..>6243
                     /locus_tag="VFR00_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0158844.1"
                     /translation="MLGSVADTRAFAPFEWLLALRYLRARRKEGFISVIAGFSFLGIL
                     LGVATLIIVMSVMNGFRKELFNKILGLNGHVIVGKAGVGFDDYAEVAQKIGAIAGVKH
                     ALPLIEGQAMVSTPAQALAGVVRGVTEDGIKALPLIAGNIRAGTLDGFGGQDGLALGS
                     RLANALRVSAGDTVTLVAPRGATTPFGTAPRIKPYRVA"
BASE COUNT         1013 a         2020 c         2071 g         1139 t
ORIGIN      
        1 cgcctttgcc gccattggtc agcgcaacat caagcggctg ctggcctact cggctatcgg
       61 caacatcggc tacatgctga tcgggcttgc cgcggcctct ccggagggcg cgcgcggggt
      121 cattatttat ctcgtgatct atgtggccat gacgcttggc gcctttgcct gcgtcttgtc
      181 gatgcgccgc gccgagggca tggtggagga tatcgatgag ctggccgggc tggcgcagag
      241 caatctcggc atggccacgg tctttggcct tctgatgttt tcgctggccg gagtgccgcc
      301 gctcgccggc ttctttgcca agttctatgt cttcgtcgcc gccgtgaagg aggggctctg
      361 gccgctcgcc atcttcggcg tgcttgccag cgtggtgggc gcctactact acgtgcgcat
      421 cgtcaaggtg atgttcttcg atgcgccgaa ggagaagttc ttggccgtcc ccgccaaagc
      481 cggcctgatc atggcgctga cgggcctgtt cgtgctgctc tatgtcgttt ggccggcacc
      541 gctggtcggc tcggcggatg cggcagccaa aagcctattt taaggagcgg cgccgcagcc
      601 cacccaagtc ccgtgccgca cgcaatccgc agcatcgtcc tcgatcaagt cggctcgacc
      661 aatgccgagg cattcgcgca ggcgatggcg ggtgaattgg gaccgctgtg gatcatggcc
      721 caccggcaaa cgcaggggcg gggtcggtcg gggcgcaggt gggtgtccga gcccggcaac
      781 ctgcatgcca gtctgctctt gcaccttgcc tgtccacctg ctgcggtgca ccagctctcg
      841 ctgttggccg gcgtggccgt gatcgatgcg gtcacgacgg cggcgggtcg acccatcagc
      901 gggctgcgct tgaagtggcc gaacgacgtc ctggtcgggg aggccaagtg cgccggcatc
      961 ctgccggaaa gccacgcggg cgctgccgcc gcacagccgc tggcggtgat tggggttggc
     1021 atcaatttgg cctggcatcc tcaggacttg gggcgcccgg tcacgcatct tgccgcgcac
     1081 ggcgtcgtgg tggacccgga agcgatgttg gcgcacttgg ccggcgccat gcagcgctgg
     1141 cttgacgtct gggattgggg tatcggtttt gccgccatac gcgaggcttg gagtgcgcgc
     1201 ggcggcccgg ccggcgaagc gctcagcgtc gacaccggca gggaaaaaat cagcggtcaa
     1261 tacctcgatc tggccgagga cggcgcgctc attatgttgg atagcaacgg cagggaacgc
     1321 aggttgacgt acggggaggt taccatcggg cgtcggggaa tatgaggcta gcgtgaccga
     1381 gcaatcgccg acagcgccaa gccgcccccc gccaggtcac gagctcgtct tcctggcgct
     1441 cggcggcatc ggtgagatcg gcatgaattg ctatctctac ggcctgggcc cggcggaggc
     1501 gcgcgaatgg ctgatggtcg atctgggcat cacctttccc gagggcgaga acgaccccgg
     1561 gatcgacgtc attctaccgg atctgcgctt catcgaggag gagcgcgccg ccttggccgg
     1621 tatcgtgctc acgcatgccc acgaggatca cttcggcgcc gtggcggagc tgtggtcacg
     1681 cctgaaagtc cccgtttatg ccacgccgtt tgcggccgcc ttgctgacgg ccaagctcgc
     1741 cgaattcggc ggccgcgagc ggccggaggt gcgggtcaag cccctcgaca gccgcttcca
     1801 gatcggcggc tttgatatcg agctcctgtc gcttgcccat tccattcccg aatccaatgc
     1861 cctcgtcatt cgcacgccgc tcgggtgcgt gctgcacacc ggtgattgga agctcgatcc
     1921 cacgccgctg attggcgccc cggccgagct tggacgcctc gcgcagctcg gcgatgacgg
     1981 catcaccgcg atcgtgtgtg attccaccaa cgccatgcgc gagggccgct cgccctccga
     2041 gcgcgacgtg gcgcgcagct tggcgggact tgttcgcgcc gccacggggc gggtggccgt
     2101 cacgatcttt gcctcaaacg tggcgcgcat ccgcgccgtc gccgacgcgg cccgaagcgc
     2161 cgggcgccac ctcgtggtgg cgggcagggc catgcaccgc attatcgatg ccgccatgga
     2221 aacgggatac cttcccagcg atttcaaata ctacgatcaa gagcactacc aatatctgga
     2281 gcgcgacgag acagtcgtgc tgtgcacggg cagccagggc gagccgcgcg ccgccatggc
     2341 ccgcattgcc gagggccagc atccccacat cagtctgggc aagggcgacc tcgtcatttt
     2401 ttcctcgcgc accatccctg gcaacgagcg cgccgtcggg cgcatccaca atcagctgat
     2461 cgatcttggc tgcgagctcg tgaccgatgc cgatgccctg gtgcacgtca ccggccatcc
     2521 gcgccgcgag gaactgaagg agatgtatgc ttgggtcagg ccgcgcgtgg cgatccccat
     2581 gcatggggag gcgcggcatc tcgccgagca tgccaaactg gcacgcgccg cgggcgtcaa
     2641 agaggttgtt ggcgtgcgca acggcgatat cgtgcggctg gcgccgggcg cggcggcagt
     2701 catcgatgag gcgccgatcg gccgcatctt ccgcgacgga cgactgctcg tctccgccga
     2761 cgagggtccg gtgcggcaac gccgggcgct ggcgtttgcc ggcatcgtca tagtcgctat
     2821 cacgctctcc gggcgtgggg agctgttggc cgaacccgta attacgcttg atggcgtacc
     2881 agccttcgat gctgccggcc aaagcgtgct cgagcatctg ctcgacgccg tcgaggccac
     2941 cctgaagagc atcccgccgc caaggcgcaa ggacggggag accgtgcgcg aagccgtgcg
     3001 acgggcggtt cgcgcccgcg tcgaagacct gtggggcaag cgccccatca ccaaagtgct
     3061 cctgacgcag ctcggcatgc gcggctgagg cagagttgtg aagcgcggag gagaaccatg
     3121 attggtcgtc tcaaccatgt cgccattgcg gtccccaagc tggccgaggc ggcaacgctt
     3181 tacgccaacg cgctcggcgc caacgtctcc cagccggtgc cccaccccga gcacggcgtc
     3241 acggtggtgt tcgtgacgct acccaacacc aagatcgagc tcttggagcc tttgggcgag
     3301 cgctcgccca ttgcggcctt tctggagcgc aaccccagcg gcggtatcca ccacgtttgc
     3361 tacgaggttg ccgatcttgc tgccgccagc gcccagctca aggccgcggg cgcgcgcgtg
     3421 ctcggcgacg gcgaacccaa gatcggcgcg cacggcaaac ccgtgctgtt cctgcacccc
     3481 aaagatttca acggtaccct gatcgagatc gagcaagcct gaggtcccca tgaccccgac
     3541 gctggccatc gccatttacg tgctcttgtg gtgggtggtg ctgtttgccg tcctgccgct
     3601 cggcgtgcgc acccagggcg aggatgggac cgtcgttccg ggtacgccgg aaagcgcgcc
     3661 gacggctccc cgtttcttgc gcatcctgtt gctgaccacg ctggtgacgt cgctgctgtt
     3721 tggcgctttc tgggcgacca tccggtacga cttgatcgat tacgcctatt ggctcggggg
     3781 cgccacgccg ccctgaggca gaaacgaaaa gagcaaggca gcacgccttg ctcaagcatt
     3841 cgcatattat attggtgtcc tcggcgccgg tttggccgac accagacacg tgtccttccc
     3901 aagacttggg ctaggcgccc ttttgcacag caagcgggct tgcgcccgtc gtaattgcgt
     3961 acacagtagt caaggcgcgg ccttgtgtca cccctccagc gctccaccac aagcattttt
     4021 tcctgctggt aaaggatcga gtgtgccccg gctcaggcta cgcgcgagcc ggagttgccc
     4081 caggggcggc ccggagctgg agcgggcttg ctcagccccg cggcagccgg agccaagttg
     4141 cgcccccggg cagaacacga taagagccct caacttgcgt gcctcggcgc acgtctgccc
     4201 tcgttcctgc atagttctaa agcaacgtcg gtcattgcct agaaagcgac caaccaaccc
     4261 gccatgcgcc tttcacgctt ttttttgccg gtcctgcgcg aaacgccaaa ggaagcagag
     4321 atcgtttcgc atcagctgat gctgcgcgcc ggcatggtgc gccaggcgag tgccggcatt
     4381 tattcctggt tgcccctagg gctgaaggtc ttgcagcgca tcgaaacgat cgtgcgcgag
     4441 gagcaggacc gttccggggc gatcgaaatg ctgatgccca ccctgcagca ggccgacctg
     4501 tggcgcgaga gcgggcgtta tgatgcctac ggaccggaaa tgttgcgctt tcgtgaccgg
     4561 cacgagcgcg agatgctcta tggcccgacc aacgaggagc tgatcacgca gatcttccgc
     4621 gacgcggtgc gctcctatcg cgacctgccg cgcaatctct accacatcca atggaagttc
     4681 cgcgacgagg tgcggccgcg tttcggcgtc atgcgcgggc gcgagtttct catgaaggat
     4741 gcctattcct tcgacatcag cgcggcggcg gcgcggcagg cctaccaccg catgttcgtg
     4801 gcctatctgc gcacctttgc gcgcctgggc ctgaagtcga ttcccatggc cgccgacacg
     4861 ggcccgatcg gcggcgatct cagccatgaa ttcattatcc tggccgagac gggcgagagc
     4921 cgcgtctact gccatgccga tctcttggac ctgcgcgtgc cgcccgccga tgtcgatttt
     4981 gccggcgacc tggcacccct gatcgagcag tggaccaagc cctacgcggc caccgacgaa
     5041 aagcacgagc aagcccgctt cgccgcggag gtgcccccac agaaacgcgt gtcggcgcgc
     5101 ggcatcgagg ttggccacat tttcttcttc ggcaccaagt attccgagcc gatggggtgc
     5161 cgcgtgcagg gaccggatgg cagcatgatt gcggtgcaaa tgggctccta cggcatcggc
     5221 gtttcccggc tggtcggcgc cattatcgaa gccagccacg atgccgccgg catcgtctgg
     5281 cccgtgccgg tggcaccgtt cgaggttgcc ttgatcaatc tcaaggccgg cgacaaggac
     5341 acggacaggg cctgcgaaga cctttatgcc aggctcatgc gcgccggggt ttcggtgctt
     5401 tatgaggaca ccgaggagcg ggccggggca aagttcgcca gcatggacct gatcgggctg
     5461 ccgtttcagc tgatcgtcgg tccgaagggc gtcaaaaatg gcgaggtcga agtcaaggag
     5521 cgcaggtcgg gcgaacgcgt caacttggcg cccgacgcgg ccgtgaagcg cctggtcgag
     5581 ctcatcagtg cgcggcgcct cttggtctag agcgaagggg tcgggggcaa caacaagagg
     5641 cttggggaga tgctggggtc ggtggccgat accagagcgt ttgcgccctt cgaatggctg
     5701 ctggcgctgc gctacctgcg cgcgcggcgc aaagagggct tcatctcggt gatcgccggc
     5761 ttttccttcc tcgggatcct gctcggcgtg gcaaccctga tcatcgtcat gtcggtgatg
     5821 aacggcttcc gcaaggagct gttcaacaag atcctgggcc tcaacggcca tgtgatcgtc
     5881 ggcaaagccg gggtcggctt cgatgactac gccgaggtcg cgcaaaaaat cggggccatt
     5941 gccggcgtca aacacgccct gccgctgatc gagggccagg ccatggtgtc gacgccggcg
     6001 caagcgctgg cgggtgtcgt gcgcggggtg accgaggacg gcatcaaggc cttgcccctg
     6061 attgccggta acattcgggc cggcacgctc gatggtttcg gggggcagga cgggctggcg
     6121 ctgggctcgc ggctcgccaa tgccctgcgc gtgagcgccg gcgataccgt caccctggtc
     6181 gcgccgcgcg gggccaccac gccctttggc acggcgccgc gcatcaagcc ctatcgcgtg
     6241 gcc
//
LOCUS       DASTMT010000258         4159 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_796114, whole genome shotgun sequence.
ACCESSION   DASTMT010000258 DASTMT010000000
VERSION     DASTMT010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4159
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_796114"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(70..900)
                     /gene="otsB"
                     /locus_tag="VFR00_06020"
     CDS             complement(70..900)
                     /gene="otsB"
                     /locus_tag="VFR00_06020"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HEU0158845.1"
                     /translation="MRASIAMQDVREDISDRAGAVLANPRDWALFLDIDGTLLRMAPT
                     PDAVAVPPELPPLLAALAVGLEGALAIVTGRRVAEADRLLAPVKLVASGVHGTELRED
                     PQGPIRMLAEPVPADVIEMMTSVSRIAEGILVEQKGSGAAVHYRNAPAAQRAIESEVA
                     RIIGLSSYDLVLRRGRKVLEAVPRGFSKGQALMDLMRRPPFKGRRPLMIGDDVGDETA
                     LKAADALSGMGLKVAGEHFAPDEADFGGVQEVRTWLGLLAERLGQKPADNWPARACGG
                     "
     gene            complement(932..2026)
                     /locus_tag="VFR00_06025"
     CDS             complement(932..2026)
                     /locus_tag="VFR00_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HEU0158846.1"
                     /translation="MKALTVGGAMIDTIAIIASDRIERMSMRNADSSFLLLEEGRKTE
                     ALEVSTHVGGGAVNAAVAMARLGLDVAALVKLGKDARAETVLNHLLQEGVSTRWAMRD
                     GRAPTGASVLLSSHERNAGIFTFRGANTLLDASELRAEAFAVDLVYVSSLSNESADAF
                     PAIVKLAKAQRALVATNPRVRQLSSRGNAFIESLANIDILTVNSSEADVLVPALVARF
                     GEGGKALPLKAGEIPPRLAARGFTSGGLDVSLVAFFAALRQLGPRYVVVTDGRDGAYL
                     GTADAILYCPVLESQVAGTAGAGDAFNATFTAYIASGRPAADALLAAAINAAAVVGHI
                     DTQTGLLAREAIDERVTAATKTLAVRTWSH"
     gene            complement(2023..2841)
                     /locus_tag="VFR00_06030"
     CDS             complement(2023..2841)
                     /locus_tag="VFR00_06030"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEU0158847.1"
                     /translation="MAATTSAGPNTEAHADRPAAVPPVTLGVDIGGSNIKVAAVDASG
                     RLLAEQVRTPTPKPATPEAVLAAIGKLVAASPGFDRISVGFPGVVRGGEVITAPNLGT
                     PFWAGFAMIAALAERFGVPVRMLNDAVVQGLGVVEGPGLECVVTLGTGVGCALFRNRR
                     LLVQLELGQHPARKGKSYDQYAGQAALAVKGAAAWNRRVRKVITSVLDLTNCDRLYIG
                     GGNARKIAFQPPPQVKIVSNAAGITGGVRLWEEALDDFFVGVPMADASRYQRGR"
     gene            complement(2863..>4159)
                     /locus_tag="VFR00_06035"
     CDS             complement(2863..>4159)
                     /locus_tag="VFR00_06035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HEU0158848.1"
                     /translation="FGWSGEIAPEHATASLEREGRIATVPLSEADHQAYYLGYSNSVL
                     WPVFHNRLDLAQFEAGFFERYVGVNRRLAEQLRPLLRPDDLIWVHDYHLLPFATELRR
                     LGALNPIGYFLHIPFPPWQTFMAIPEHQQLARALAAYDLIGLQTKADVANLVTYLENG
                     VYGRIVPDGRIRLLDRLLTVGSFPIGIDVADFAHAKRDSSLVQARAPVHRIIGVDRLD
                     YTKGLPQKFKAFGQFLDSNPGYRGQVVLTQIAPPTRGSVEAYTDIRQQLESLAGSING
                     RFGELDWVPIHYIHRSTARRRLAGIYRSSRIGLVTPLRDGMNLVAKEYVAAQDAADPG
                     VLVLSQFAGAAEEMSQALIVNPYNIAETADVIRMAFEMALPERQKRHAALFETVEKNN
                     VVAWCRSFIAQLERVRSNDPASWRAPEPIRAALAKLQAT"
BASE COUNT          707 a         1376 c         1415 g          661 t
ORIGIN      
        1 ctcggccggg cttcgcgtct gtgtcatgag taaggaggcg aggagggaag gaggcgagga
       61 gaggcggcgc tagccgccgc aggcgcgagc cggccaattg tcggcgggtt tttgccccag
      121 ccgttccgcc agcaatccga gccaggtgcg cacctcttgc acgccgccga aatcggcctc
      181 gtccggagca aagtgctcgc cggcaacctt gaggcccatg ccgcttaagg cgtcggcggc
      241 cttgagtgcc gtctcatcgc cgacgtcatc accgatcatc agcggacgcc ggcccttgaa
      301 gggtggccgg cgcatcaggt ccatcaaggc ctgacctttc gagaagcccc gcggcacggc
      361 ctccaagacc ttgcggccgc gccgcagtac caggtcgtag gacgacaggc cgatgatgcg
      421 cgccacctcc gactcgatgg cccgctgggc ggccggagcg ttgcggtagt gcaccgccgc
      481 gccagagccc ttctgctcca ccaggatgcc ctcggcgatg cggctaacgc tcgtcatcat
      541 ttcgattacg tcggcgggca ccggctcggc cagcattctg atgggaccct ggggatcctc
      601 ccgcaactcg gtgccgtgca cgcccgaggc cacgagcttg accggcgcca gcagccggtc
      661 ggcttcggcc acacggcggc ccgtgacaat ggcgagcgcg ccctcaagac cgacggcaag
      721 cgccgccagc aacgggggaa gctcgggcgg caccgccacc gcgtccgggg taggggccat
      781 gcgcaagagg gtgccgtcga tgtcgagaaa cagcgcccag tcgcgcgggt tggccaaaac
      841 ggcccctgct cggtcgctga tgtcctcccg cacgtcctgc atggcaatgc tagctcgcat
      901 caaaaaagcg gtgcattttc gaccgtccct atcagtgcga ccatgtgcgc acggcaagcg
      961 ttttggtagc tgccgtcacg cgctcgtcaa tggcttcgcg ggccagtagg cccgtttgtg
     1021 tgtcgatgtg gccgaccacg gcggccgcgt tgatggcggc ggcgagcagt gcatccgccg
     1081 ccggccgtcc cgaggcgatg taagcggtaa acgttgcgtt aaaggcatcg cccgcgccgg
     1141 ccgtgccggc cacctgggat tccagcaccg ggcaatacag aatggcatcg gccgtgccga
     1201 ggtaggcgcc gtcgcggcca tcggtcacca ccacatagcg cgggcccagc tgccgcaagg
     1261 cggcaaagaa ggcgacgagg ctgacatcga gcccgccgct ggtgaagccg cgcgcggcaa
     1321 gccgcggcgg gatttcgccg gctttgagcg gcaaggcctt gccgccctcg ccgaagcggg
     1381 ccaccagggc gggaaccagc acgtccgcct ccgagctatt gacggtcaag atgtcgatat
     1441 ttgccaggct ttcgatgaag gcattgccgc gcgaggacag ctggcgcacg cgcggattgg
     1501 tggcgaccag cgcccgctgg gccttggcca acttgacgat ggcgggaaag gcatcggccg
     1561 actcgttgct gagggaggac acgtagacga ggtcgacggc aaaggcttcg gcgcgcagct
     1621 cgctggcatc gagcagggtg ttggcgccgc ggaacgtgaa aatgccggcg ttgcgttcgt
     1681 gcgaagacaa cagcaccgag gcgccggtcg gggcgcgtcc atcgcgcatg gcccagcggg
     1741 tcgagacacc ctcttgcaac agatggttga gcaccgtttc ggcgcgggca tccttgccga
     1801 gcttgacgag agcggcaacg tcgagcccca gccgggccat ggcaacggcg gcattgaccg
     1861 cgccgccgcc aacgtgggtc gaaacttcca gggcctcggt cttgcgccct tcctccagca
     1921 gcaggaacga gctatcggca ttgcgcatgc tcatgcgctc gatgcgatca gaggcgatga
     1981 tcgcaatggt atcgatcatg gctccgccga ccgtcagtgc cttcatcgcc ccctctggta
     2041 tcggcttgca tccgccatcg gcacgcccac gaagaaatcg tcgagcgcct cttcccacag
     2101 tctgacgccg ccggtgatgc ccgcggcatt gctgacaatc ttcacttgcg gcgggggctg
     2161 aaacgcaatc ttgcgggcgt tgccaccgcc aatgtagagc cgatcgcagt tggtgaggtc
     2221 caataccgag gtaatcacct tacgcacgcg gcggttccac gccgccgcgc cctttacggc
     2281 caaggccgcc tggccggcgt actggtcata gctcttgccc ttgcgggcgg ggtgctggcc
     2341 gagctcgagt tgcaccagca gccgccggtt gcggaagagc gcacatccga cccccgtacc
     2401 cagcgtcacc acgcattcca ggccggggcc ctcgaccacg cccaggccct gcaccacggc
     2461 atcgttgagc atgcgcacgg ggacgccgaa gcgctccgcc agcgccgcaa tcatggcaaa
     2521 acccgcccag aacggcgtgc cgaggttggg ggcggtgatg acctcgccgc cgcgaacgac
     2581 gcccggaaaa cccacggaaa tgcgatcgaa gccgggcgag gcggcaacca gcttgccgat
     2641 ggcggccagc acggcctccg gggtcgccgg cttcggggtt ggcgtgcgca cctgttcggc
     2701 cagcagcctg ccgctggcgt cgaccgccgc caccttgatg ttgctgccgc cgatatcgac
     2761 gccaagcgtc acgggcggaa cggccgccgg cctatccgcg tgcgcctcgg tgttgggccc
     2821 ggctgaggtc gtcgctgcca tccgctgctg cgccatcact cctcatgtgg cctgcagttt
     2881 tgccaaggcg gcgcggatgg gctcgggcgc acgccagctc gccgggtcat tgctgcgcac
     2941 gcgctcgagc tgtgcgatga acgatcggca ccaggccacg acgttgttct tctccaccgt
     3001 ctcgaacagg gcggcgtgcc gcttctggcg ctccggcagc gccatctcga aagccatgcg
     3061 gatgacgtcg gcggtctcgg cgatattgta gggattgacg atcagggcct ggctcatttc
     3121 ttccgccgca cccgcaaact gcgacagcac gagcacgcca ggatcggctg cgtcctgtgc
     3181 cgccacgtat tccttggcaa cgagattcat accatcgcgc aggggggtga caagtccgat
     3241 gcgcgaagag cggtagatgc cggcgaggcg ccggcgcgcg gtggagcgat ggatgtaatg
     3301 gatcggcacc caatcgagct cgccgaagcg gccattgatg gagccggcaa gcgattccag
     3361 ctgctggcgg atgtcggtgt aggcctcgac gctgccccgc gtcggcggcg cgatttgcgt
     3421 cagcacgacc tggccgcgat agccggggtt gctgtccagg aattggccga aggccttgaa
     3481 cttctgcggc aagcctttgg tgtagtcgag ccgatcgacg ccgatgatcc ggtgcacggg
     3541 cgcgcgcgcc tgcaccaggc tgctgtcgcg cttggcgtgc gcgaaatcgg cgacatcgat
     3601 gccgatcggg aagctgccca ccgtcagcag ccgatcgagt aggcggatgc gcccgtccgg
     3661 cacgatgcgg ccgtagacgc cgttttccag gtaggtgacg aggttggcaa cgtcggcctt
     3721 ggtttgcaga ccgatcaagt cataggccgc cagcgcccgc gcgagctgtt ggtgctcggg
     3781 gatggccatg aaggtctgcc aaggcggaaa cggaatgtgc aggaaatagc cgatcgggtt
     3841 cagggcccca agccggcgca gctcggtggc gaagggcagc agatggtagt catgcaccca
     3901 gatcaggtcg tcgggccgca gcagcggccg cagctgctcc gccaggcggc ggttgacgcc
     3961 gacgtagcgc tcgaaaaagc cggcctcgaa ttgcgccagg tccagacgat tgtggaagac
     4021 cggccacaac accgagttgc tgtagcccag gtaataggcc tggtgatcgg cttccgacag
     4081 gggaacggtg gcgatgcggc cctcacgctc caggctcgcc gtcgcgtgct cgggcgcaat
     4141 ttcgccgctc cagccaaac
//
LOCUS       DASTMT010000259         6686 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_1777098, whole genome shotgun sequence.
ACCESSION   DASTMT010000259 DASTMT010000000
VERSION     DASTMT010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 6686)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6686)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6686
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1777098"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(680..826)
                     /locus_tag="VFR00_06040"
     CDS             complement(680..826)
                     /locus_tag="VFR00_06040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0158849.1"
                     /translation="MKQSIHRMQRGVWRACQVELWQLSEPAVQAIDEIVRRVLSEQKQ
                     KAGA"
     gene            complement(839..1024)
                     /locus_tag="VFR00_06045"
     CDS             complement(839..1024)
                     /locus_tag="VFR00_06045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0158850.1"
                     /translation="MHGFRRSLRDWATKVGEVREVVAEAALAHTVGDKTEAAYRRTTY
                     LKERRDLMQLWANYCSA"
     gene            complement(1492..3012)
                     /locus_tag="VFR00_06050"
     CDS             complement(1492..3012)
                     /locus_tag="VFR00_06050"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HEU0158851.1"
                     /translation="MNEDAVYRLTLAQRILILVTVMLSNTIYSGSVLISSALLPQLQG
                     ALSATQDEISWVMTFNIVATAVATPATGWLADRFGQRRVMISSALLFTTATFLCGASN
                     SLEELIFWRIAQGAAGAPLVPLGQTVLFDSFPRRQHSTVISVYGMANMVGPSLGPMFA
                     GQIAETFGWRWGFWMILPVGVAAIVGCWSALPREERKAQAHLDWLGFISLSSGIAAAQ
                     LVFSRGQRLDWFDSIEIVLASIGAGLMLYFFCVHSLTSDRPFVRLRLLADRNYALGLG
                     LVTLFGMLNFAIIVLLPPLLQQHAGYPESAIGEIIGWRGLGSAIGFLLAMPMGRLDPR
                     VSLSIGALLQMLTGLWLMSFDLNVGMTALLLCNTLQGMSIGVSWVPLTVITFWTLSPK
                     YRAEAMSMFHLLRNFGSSLFISVAVAEIVRSQSANYARMVEAISPYNRVLELPWVMGA
                     WSVDSTAGLAKLSSEIARQSILIGYTNAWLLYTLAAAAWLPLCMLARLPRANPKPL"
     gene            3090..3587
                     /locus_tag="VFR00_06055"
     CDS             3090..3587
                     /locus_tag="VFR00_06055"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEU0158852.1"
                     /translation="MTSDDPQFIGYTITDVGRLLRTVFERRVRGFGLTRAQWLIIARL
                     HRRPGLSQSEIADLLEIEKAPAGRLIERMEAKNWLQRRSDAKDKRVNRLYLTSKANRL
                     HAAIWPIAEATVEDALGDLSPEERRRLSALMARVKMTLQGLAERDPARGEAADEAERE
                     ELRAL"
     gene            3620..4654
                     /locus_tag="VFR00_06060"
     CDS             3620..4654
                     /locus_tag="VFR00_06060"
                     /inference="COORDINATES: protein motif:HMM:NF012739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HEU0158853.1"
                     /translation="MLLGVVPALVILAALVVWLWGGRYVSTENAYVKADIAQISAEVS
                     GRVLEVHVEDHAEVKVGDLLLTLDAEPFHVALSKADAELDSMREQVRTLMASWHEAQS
                     ELREAESKVAYWNAQLARQKTLSERGIVASSKFEEVESNATSAEDRVAIMRRRLDRVA
                     AQLGGDPTRAEDDHPMVRQKRAERERAALDLAHTIIRAPLGGTAVNVRLQKGEQIKAA
                     TPLFALVADTRPWVEANFKETELTHVRKGQAAQVVLDIYPDVVWQAEIESISPATGAE
                     FAVLPPQNASGNWVKVVQRLPVRLRLLPQPGEPPLRAGMTASVRVDTERQSRLSRLIG
                     GWSAFATSGN"
     gene            complement(4675..6015)
                     /locus_tag="VFR00_06065"
     CDS             complement(4675..6015)
                     /locus_tag="VFR00_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018318008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEU0158854.1"
                     /translation="MAKRQLSRRQFVGTAAAATSLVAAPFVRTAGAAGSLSLGLWDHW
                     VPGGNTAMTEICQAWADKEKVNLQIDYLSTQGNKLYLTIAAEALAKSGHDIMDFSAWE
                     PPHYETSLEPVDDVMQEVLSRNGPVSPAIEYLAKPKGKWLVVPGIRGTLCLTACSRID
                     LMKEHAGIDLLSMFPAGGEPSKAVEDWTWDNFLLAAERCHKAGYPFGLPLGGTTDSVD
                     WLGAFLRAFGAELVNEKGEITAKSDAMRQALEYLNRLSAFLPPDVSAWDNASNNKWLV
                     AGKGALIFNPPSAWAVARRDAPQVAEKLWTHAMPKGPKGRFVSVLPRFQGIWNFSKNK
                     SAAKALLQHVSTREAAAKLVDGGQGYDLPPFVKFNDFKTWDEQVPPKGTLSHYPNKGD
                     QVSIVPFAPAPPGVAQQIYTQALASKMVVRMMKGEPMAKTLDWAVAELEGFSRN"
BASE COUNT         1223 a         2124 c         2195 g         1144 t
ORIGIN      
        1 tcttaaggaa ggttgctaac tgggcctggc acgacataac aatgtggccg ccccgatcaa
       61 ggtggcggcc atgttttacg tgtcagtagt ggcgcactag ccgcccgtgt acgtcatgaa
      121 ccaggagtgc cgtttggcac cgggtagacg ctctggcgct tgacgccgag ctgcccaggc
      181 gatcgcgcaa acgcttttcc tcgggctaac aggtcctgca cctggcgcag gctaaggtta
      241 cgcacccggc cgcggcattt gccgagctgc ttggcgcgcg cgatgctctc gcgctagctc
      301 tccgtcattg cgctcccttg ctgacgttgc cacccgggcc atgaccgcaa ggatcagcgg
      361 cggcgtcgta tcgaccggcg actgcatgcc ggcgtaccct tttgcaccga tcgcgtcggc
      421 taattggccg ggccgcggcc ccctacaacc cgatcacgcg gcctcgtcca aggcgccgcc
      481 ggacgcggcc taaccctggc gctgaaccca cgcatcgagc gcggcgattg gccaccggcg
      541 atgcttgttg ccgtctttcc ttttgccgac agtgatcgcc atggcttcta actcgggatg
      601 ctcatttagc atgtcgtaaa agcgggtcct gccgacgccc aggaacgttg cagcttgcct
      661 tgggcagagc agcccgctgt caggcgccgg ctttctgctt ctgctcagac agcacccggc
      721 gcacgatctc gtcgatcgcc tgcaccgcgg gctcggagag ttgccagagt tcgacttggc
      781 aggctcgcca cacgccgcgc tgcatcctgt ggatagattg cttcacgtaa aacatgtttc
      841 acgcggaaca atagttcgcc cagagctgca tgagatcgcg gcgctctttc aagtaggtcg
      901 tccggcggta ggccgcttcg gtcttgtcgc ccacggtgtg cgccagcgcc gcctcggcaa
      961 ccacctcgcg aacctcgccg acctttgtag cccaatctcg caacgagcgc cggaaaccat
     1021 gcacagtgac ctgtccttca gcctcaggtc gagaagaacc cagggccagt gtgttgtccg
     1081 acagctgcct cgccggtttg ggggctcgga aatagcaacc cgtagggtca atcgagcttg
     1141 cgcgcctgct tgaggacttc caggctgcgc tccggcagcg gcacgatgtg ctcgcggcga
     1201 cccttcatgc gcttcttggg gatcgtccag atggcttgcc gctggttgat ctcatcccag
     1261 cgcgcgcccc tggtcttgtt tgagcgagtt accgtcagga ccagccattt gaaggccagc
     1321 tttgtggcct gcccccagtt tgcatggtgc gcagtttctt gatgaacgtg gagacctcag
     1381 catgggcggg gcgcggaagt gttcgacgtc gtgattctgc ggaccaacgc gaactaaaaa
     1441 gcgctctaac ccgttgagca tcgcaggact atgccctgcg ggtgcgtggg attacaaggg
     1501 cttaggattg gcgcgcggca gccgggccag catgcacagc ggcaaccagg ccgcggccgc
     1561 cagcgtatag agcagccagg cattggtgta gccgatcaag atcgactggc gcgcgatctc
     1621 gctcgacagc ttggcaaggc ccgccgtgct gtcgacgctc caggccccca tcacccacgg
     1681 cagctcgagg acgcgattat aaggcgagat ggcttccacc atgcgcgcat agttcgccga
     1741 ctgggaacgg acgatctcgg ccaccgcgac ggaaatgaac aagctcgagc cgaaattgcg
     1801 cagcaggtga aacatcgaca tcgcctcggc ccgatacttg ggcgacagcg tccaaaaggt
     1861 gatgaccgtc agcggcaccc aagacacccc gatcgacatg ccctgcagcg tgttgcacag
     1921 caacagtgcg gtcatgccca cgttgaggtc gaagctcatc agccacaatc ccgtcaacat
     1981 ctgcaacaaa gcgccaatcg aaaggctgac gcggggatcg agacggccca tcggcatggc
     2041 aagcaggaag ccgatggccg agccgaggcc gcgccagccg atgatttcgc cgattgccga
     2101 ttcgggatag ccggcatgct gttgcagcag cggcggcagc aaaacgatga tcgcaaagtt
     2161 aagcatgcca aacagggtga ccagccccaa gcccagcgca tagttgcggt cggcgagcaa
     2221 gcgcaggcgc acgaaaggtc ggtcgctggt caggctgtgt acacagaaaa agtaaagcat
     2281 gaggccggcg ccgatgctgg cgagcacgat ctcgatgctg tcgaaccagt cgagccgctg
     2341 gccgcgcgag aacacgagct gagcggccgc aattccggag gaaaggctga tgaagccaag
     2401 ccaatctaga tgcgcttgcg ccttgcgttc ttcccgcggt agcgccgacc aacagccgac
     2461 gatggcggca acgccgacgg gcaaaatcat ccaaaacccc cagcgccatc cgaacgtctc
     2521 ggcgatctgg ccggcgaaca tgggcccgag cgagggcccg accatgttgg ccatcccata
     2581 aaccgagatc accgtgctgt gctgtcggcg cgggaagctg tcgaacagca cggtttgccc
     2641 gagaggcacc agtggggcgc ccgccgcgcc ctgggcaatg cgccaaaaga tcaactcctc
     2701 gagcgagttg gaggcgccac acaggaaggt ggcggtggta aagaggagcg cgctcgagat
     2761 catgacgcgg cgttgcccga accgatcggc aagccaaccc gtggcggggg tcgccaccgc
     2821 cgtggcgacg atattgaacg tcattaccca ggagatctcg tcctgggttg ccgacagcgc
     2881 cccttgcaac tgcggcagca aggcggaaga aatgagcacc gatcccgagt agatcgtgtt
     2941 gctcaacatc accgtgacga ggatcaaaat tcgctgcgcc agcgtcagcc gatagacggc
     3001 atcctcgttc atccgcgagc cgatcgtcgt cttgacaaat acaccggctg atatagtaac
     3061 ctaggttact aatttgcgcc aggcctcgca tgacgtcgga cgatccgcag ttcatcggct
     3121 acacgatcac cgatgtgggg cgcttgttgc gcaccgtatt cgagcggcgc gtgcgcggct
     3181 tcggattgac gcgggcgcag tggctgatca ttgcgcgcct gcatcggcgc ccggggctga
     3241 gccaaagcga aatcgccgat ctgctcgaga tcgagaaggc gccggcgggg cggctgatcg
     3301 agcgcatgga agccaagaac tggctgcagc gccgcagcga tgccaaagac aaacgcgtca
     3361 atcggctcta cttgacgagc aaggccaatc gtctgcacgc ggcgatttgg ccgatcgcgg
     3421 aggcaaccgt cgaggacgcc ttgggcgacc tgtcgccgga agagcgccgg cgactgtcgg
     3481 cactgatggc acgcgtcaag atgaccctgc aggggctggc cgaacgcgat ccggcgcgcg
     3541 gcgaggcggc ggacgaggcc gaaagagagg agctgcgggc gctgtgagcc ggcttttgca
     3601 tcgccgatat gtgcggccca tgttgctcgg cgtggtgccc gctctggtga tcctggccgc
     3661 cctggtcgtg tggctctggg gtgggcgcta cgtcagcacc gagaatgcct acgtcaaagc
     3721 cgatatcgcg cagatctccg ccgaagtcag tggtcgtgtg ctcgaggtgc atgtggagga
     3781 tcatgccgaa gtcaaggtcg gcgatttgct gctgacgctg gacgccgagc ccttccacgt
     3841 cgccttgagc aaggccgacg ccgagctcga tagcatgcgt gaacaggtac gcacgctaat
     3901 ggcatcctgg cacgaagcgc aaagcgagct gcgcgaggcg gagagcaagg tcgcctactg
     3961 gaatgcccaa ctcgcgcggc aaaagacctt gagcgagagg ggcatcgtcg cctcgagcaa
     4021 gttcgaggag gtcgagagca acgccacgtc ggccgaggat cgcgtggcca tcatgcgtcg
     4081 aaggcttgac cgcgtcgccg cgcagctggg cggcgacccg acgcgtgccg aggacgacca
     4141 tccgatggtg cggcaaaaac gggccgagcg ggaacgggcg gcgttggatt tggcacacac
     4201 catcatccgt gcgcccttgg gcggcaccgc ggtcaatgtg cggttgcaga agggcgagca
     4261 gatcaaggcg gccacgccgc tcttcgccct ggtcgccgat acacggccgt gggtggaagc
     4321 caacttcaag gaaaccgagt tgacgcacgt gcgcaaggga caggccgcac aagtcgtgct
     4381 cgacatctat cctgacgtgg tttggcaggc cgagattgaa agcattagcc cggccaccgg
     4441 cgccgaattc gccgtgttgc cgccgcagaa cgcctcgggc aattgggtaa aggtggtgca
     4501 gcggctgccg gtgcggctgc gattgctgcc ccaacccggc gagccgccgc tgcgcgccgg
     4561 catgaccgcg agcgtgcggg tcgataccga gcgccagagc cggctcagcc ggctgatcgg
     4621 gggctggtcc gccttcgcga ccagcggcaa ctaggggtcc gcgccccgcg ccggtcagtt
     4681 ccggctgaag ccctccagct cggcgacggc ccaatcgagg gtttttgcca tcggctcgcc
     4741 cttcatcatg cgcaccacca tcttcgaggc cagcgcctgc gtatagattt gctgggcaac
     4801 gccgggcggc gccggcgcga atgggacgat cgagacctga tcgcccttgt tggggtaatg
     4861 cgacagggtc cctttgggcg ggacctgctc gtcccaggtt ttgaaatcgt tgaatttgac
     4921 aaacggcggc agatcatagc cctggccgcc gtcgaccagc ttggccgcgg cctcgcgggt
     4981 cgagacgtgc tgcagtagcg ccttggccgc cgacttgttc ttggaaaagt tccagatgcc
     5041 ctggaagcgc ggcaacaccg aaacgaacct gcctttgggt cccttcggca tggcgtgggt
     5101 ccagagtttt tcggccacct gcggggcatc gcgcctggcc accgcccagg cgctgggcgg
     5161 gttgaagatc aatgcgccct tgcccgcgac cagccatttg ttgttggagg cattgtccca
     5221 ggcgctgaca tcgggcggca ggaaggcaga gaggcgattg aggtattcga gcgcttggcg
     5281 catagcgtcc gacttcgcgg tgatctcgcc cttttcgttg acgagttcgg cgccaaaggc
     5341 gcgcaagaag gcgccgagcc agtcgacgct gtcggtcgtc ccaccgagcg gcaacccgaa
     5401 cggatatccc gccttgtgac acctttcggc ggcgagcaag aagttgtccc aggtccaatc
     5461 ctcgaccgcc ttgctgggtt cgccgccggc aggaaacatc gacaacaggt cgatgcccgc
     5521 gtgctccttc atcaggtcaa tgcggctgca ggccgtcaag cacaaggtgc cgcggatccc
     5581 tggcaccacc agccactttc ccttgggctt ggcaaggtat tcgatggccg gactgacggg
     5641 cccgttgcgg ctcagcacct cttgcatgac gtcgtcgacg ggctcgagcg aggtctcgta
     5701 gtggggcggc tcccaggccg agaagtccat gatgtcgtgt cccgactttg ccagcgcctc
     5761 cgccgcaatc gtcaggtaga gcttgttgcc ctgggtcgag aggtagtcga tctgtaggtt
     5821 gaccttctcc ttgtccgccc aggcttggca aatctccgtc atcgccgtgt tgccacccgg
     5881 cacccagtga tcccataggc cgagactgag gctgccggcg gcaccagccg tgcgcacgaa
     5941 gggggcggca accagcgacg tcgccgccgc cgccgtaccc acgaattgac gacgtgaaag
     6001 ttggcgcttg gccatcagct acctcccaaa caaaatgacg tcgggcctgc acgaggccca
     6061 tcggcagcct gctcggcgcc gcatttttag gcgcggcatg ctaatcgagc ggccggcccg
     6121 gcgctaggcc catcgccgta ggccgctcag ccggcggcgc gcaggtccca agcgcttccc
     6181 gcacgtcccg agagcccggc gggcttggcg aggaggctct gccaccagtt gagggcctgt
     6241 tggggtcaaa gcttggaaca gctggaagag ccgcggcatc ggccgttcgg tctcgattgg
     6301 gcggattggg ccccgtggcc gcgccgactg gcggctgcgc cgacgaggac ggttggggcg
     6361 ggcgcgggat cgggacggcc gggaagcgat gggcccaggc gaagcgcacc cgacgctttg
     6421 gcccagcgcg cagttttcct ttcgcccgcc ctcgcggcgc gctttcccgt gcgccgccgc
     6481 cgacctcggg ctggtcgcgt cggggcagcg cctccgcggg cacgcctcgc gcctcgatga
     6541 tggcgcgcgc agcgttttgg cggggctccg cacgataagg cgcgggccta agccaagccg
     6601 gcagcggcca gcttggttcg gatcgcctcg cgctcgaagg gcttcaggat atagtcgtcg
     6661 gccccgctgc tcagcgccag ggcgat
//
LOCUS       DASTMT010000260         7310 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_2433121, whole genome shotgun sequence.
ACCESSION   DASTMT010000260 DASTMT010000000
VERSION     DASTMT010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 7310)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7310)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7310
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2433121"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..996
                     /locus_tag="VFR00_06070"
     CDS             <1..996
                     /locus_tag="VFR00_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011663574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HEU0158855.1"
                     /translation="AIILVGILLSTVGTDLESGEERLTFGWSEISDGLDVAVLAMGLF
                     GFAEILRNLEVTQARDVVRAAIGNLLPNRQDFAQSAAPIARGTLIGAILGLLPGNGAV
                     LGPFASYTIEKKLADDPSRFGRGAIEGVAGPESANNAGAQTAFIPLLALGIPPNAVMA
                     LMVGAMTIHGIVPGPQIMTKQPNLFWGMITSMWIGNLMLLVINLPLIGLWVRFLKVPY
                     RLMFPAIILLCCIGIYSINNSPVEVLMTAGFGLFGYAVVKFGFEPAPLLLGFVLGRLM
                     EEKLRQALVISRGSFMTFVDRPLSAGLLLLSLITIVLAVLPAIRKGREEVFQEAD"
     gene            complement(1289..2170)
                     /locus_tag="VFR00_06075"
     CDS             complement(1289..2170)
                     /locus_tag="VFR00_06075"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011439985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HEU0158856.1"
                     /translation="MGVWQLARAKVNLTLKVLGRRSDGYHELESLVTFAAVADRLSLV
                     PGEPTHLVASGPYADAIEAPNILERALDLLRQQGVDLRGAIALEKNLPVAAGLGGGSA
                     DAAALLRLARALHAERLEEPLWREVARRLGADVSVCLADRPALIRGIGDEVDPLPPGA
                     EPLPAVLANPGVRLATPRVFAALDAPPVDRTSARDLPPAPLRCRQALFDHMGAVGNDL
                     ERPAAALEPVIATLKAALLARPGCRAAAMSGSGPTCFGIFASEDDAAAAALALRSRWP
                     RWWIVATNLDDVVSLNP"
     gene            complement(2231..4024)
                     /locus_tag="VFR00_06080"
     CDS             complement(2231..4024)
                     /locus_tag="VFR00_06080"
                     /inference="COORDINATES: protein motif:HMM:NF024821.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU0158857.1"
                     /translation="MRRWLSLLSLLSAVVAAVLAAQAVPSHAGLGAAPEAGSLLGSYL
                     AGRVARAQNDTRAAAMFYRQALAQDPGNNLLVEQAFLMETSEGEWSRAEALARELVAS
                     QPNHRTAHALLALADFKAQRYAAADEHFKAVSGNPIGELTSALARAWVAQAEGKTTEA
                     LALLDAPKLPDWAQFYLRYHRALIADVAGRRAEARAAYERIPANDQRTLRITLAYTRH
                     AANAGDAKLAQRILKAHFDKAKADGHPEAKALQDQIDAGAEVTLLVGTPTEGLAEAFY
                     GLGEALTGEGGVSVGAVYLQFALYLRPDFPFALATLANAYETTKRYQAAIDAYDRIPN
                     DTALQVSVEIRKALNLNQLERVEEAKDLLEGTAQRNPTDVRPLDALGGIMRGHKRYAE
                     AVEYYSRAIALIDKPEQKHWSFFYSRGTCYERIKKWALAEADLQRALQLSPDQPLALN
                     YLGYSWIDQGKNLKQGMALIEKAVRQKRDDGYIVDSLGWAHYRLGNFKEAVKHLERAV
                     ELRPEDPVLNDHLGDAFWRVGREREARFQWEQALTLKPEPEDTDKIKRKLQRGLPSPQ
                     QARPVKRTKEVVKKEHLKKSSETTFSLPLFK"
     gene            complement(4250..5218)
                     /locus_tag="VFR00_06085"
     CDS             complement(4250..5218)
                     /locus_tag="VFR00_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014495600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU0158858.1"
                     /translation="MPADKLHPDAQRVCEMIIASGRPPIETLTPVEARAVYLASKPIL
                     QPDPEPVAEMTDLQAEGPAGPIPLRLYRGRPAEAASPQPVVVYFHGGGWVIGDLESHD
                     QLCRALANAVPATVIAVHYRLAPEHKFPAAVEDAIAATRWIAANADRLKVDPSRLAVA
                     GDSAGGNLSAVAAIAARDSGQARPVFQFLIYPGTDMAMQSASILRHAEQLPLTRKGMQ
                     WFLNHYLNGPQDIEDWRASPLRATNLRGLPPALIITAGFDPLSDEGEAFAAALAAADV
                     PVELERFEGQIHGFITMGRIVADARRALERGAVRLGAAFGLNRSGA"
     gene            complement(5257..6936)
                     /locus_tag="VFR00_06090"
     CDS             complement(5257..6936)
                     /locus_tag="VFR00_06090"
                     /EC_number="1.5.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018185134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004174 -
                     electron-transferring-flavoprotein dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein-ubiquinone
                     oxidoreductase"
                     /protein_id="HEU0158859.1"
                     /translation="MALADAQSSPREAMEFDVVIVGAGPAGLAAAIRLKQLAAERDRE
                     LSVVVLEKGSEVGAHILSGAVIDPIGLNALIPDWRAKGAPVETEVTDDRFYYLGPAGQ
                     LRIPNLIMPPLMRNHGNYIVSLGNLCRWLGEQATELGVEIYPGFAAAEVLYDENGAVT
                     GVATGDMGLDKDAKPTERFSRGMELKGKYTIIAEGARGSLTKVLTAKFGLREGREPQK
                     FGLGLKELWEVAPERHRPGLVQHTLGWPLDDRTGGGSFLYHYGANLVSVGFVVHLNYA
                     NPYLSPYQEFQRFKTHPLISVTFEGGRRIGYGARALTEGGLQSIPRLTFPGGVLIGCA
                     AGFMNVPRIKGSHNAMLSGIAAADAAFAAIAAGRAQDRLEAYAAEVASGPITRDLRRV
                     RNAKPMLSKFGTLLGTALSGADMWANTVLPGLGFGYTLKHGKPDHATLDIAKAERPLD
                     YPRPDGTLTFDRLTNVSFSATNHEEDQPVHLKVRDPVLQRQSEHDVYAGPSTRYCPAG
                     VYEWLEEAGKLRFQINAQNCVHCKTCDIKDPNQNIDWVVPEGGGGPNYSGM"
     gene            7060..>7310
                     /locus_tag="VFR00_06095"
     CDS             7060..>7310
                     /locus_tag="VFR00_06095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0158860.1"
                     /translation="MTDQRHADLAALLGWYRAMGADEALSQSPIDWLARGEKGPGWCF
                     ALTAPAKPPAASGAPVSTPPPRPHAATPVLPPGAPQRPFA"
BASE COUNT         1185 a         2354 c         2440 g         1331 t
ORIGIN      
        1 gccattatcc tcgttggcat tctgctctcg acggtgggta ccgatctgga gagcggcgag
       61 gagcgtctga ccttcggatg gtcggaaatc tccgacggcc ttgatgttgc ggttttagcc
      121 atgggactgt ttgggtttgc cgaaattctg cgcaatctgg aggtcacgca agcccgtgac
      181 gtggtgcgcg ccgccatcgg caatctcctg cccaaccgcc aggatttcgc acagtcggcg
      241 gcgcccattg cccgcggcac cttgatcggg gcgatactgg ggctgttgcc cggaaacggc
      301 gcggttttgg gtccgtttgc gtcctacacc atcgaaaaga agctggcgga cgaccccagt
      361 cggttcggca gaggggcgat cgaaggcgtc gccgggccgg agtcggccaa caacgccggt
      421 gcgcagacgg ccttcatccc cttgctcgcg ctgggcatcc cgcccaacgc ggtgatggcg
      481 ctgatggtcg gcgcgatgac catccatggc attgtgcccg gtccgcaaat catgaccaag
      541 cagcccaacc tgttctgggg catgatcacc tccatgtgga tcggcaacct gatgctgctc
      601 gtcatcaatc tgcctctgat cggattgtgg gtgcggttct tgaaggtgcc gtaccgtctg
      661 atgtttccgg ccattattct gttgtgctgc attggcatct actcgatcaa caattcaccg
      721 gtcgaggtgc tgatgaccgc tggcttcggc ttgttcggct acgccgtcgt caaattcggc
      781 tttgaacctg cgcccttgct gcttggcttc gtgctcggcc ggttgatgga ggaaaagttg
      841 cgccaggctc tggtgatctc gcgcggcagc ttcatgacct ttgtcgatcg cccgctgagc
      901 gcaggtctgc tgctgctgtc tttgatcacc atcgtgctcg ccgtcctgcc agctatccgc
      961 aagggccgcg aggaggtgtt ccaagaagcg gactgatccg gccggcgtcg ggaaagaccc
     1021 cagtgagcgt ttgctgatcg cggccgcaag gctgcccgcc gcgagacgcg aagaacccag
     1081 ccgatccgcg gtttttcgga gccttggcgc tacggccgtt cgtggaggca tcgcgcctgc
     1141 gccacgccgc gggacctgcc gcgcgcggcc accacgggtg cggcaccgag ccatcgccta
     1201 cgcccgccgc ccctatggtc cttgccagcc ctgccgtctg aaactcggcc gggtttcaag
     1261 cgcctttcgg gctcaggacc caatctcgct agggattgag cgaaacgacg tcgtcgaggt
     1321 tggttgctac gatccaccag cgtggccacc ggctgcgcaa ggcgagcgcg gccgctgcgg
     1381 cgtcgtcctc gctcgcgaat atgccaaagc aggtggggcc gctgcccgac attgctgccg
     1441 cgcgacagcc cgggcgggcc aggagtgccg ccttcagcgt ggcgatcacg ggctccaggg
     1501 cggccgccgg ccgttcgaga tcgttgccca cggcccccat gtggtcgaac agcgcttgtc
     1561 ggcaacggag cggtgcgggt ggcagatcgc gcgcggacgt gcggtcaacg ggtggcgcat
     1621 cgagggctgc aaacacgcgc ggcgttgcca gcctcacacc cgggttggcg agcacggcgg
     1681 gcagcggttc ggcacccggc ggcaacgggt cgacctcgtc gccgatgccg cgaatgaggg
     1741 ccggccgatc ggcgagacag acggaaacgt cggcgccgag ccggcgcgcc acctcgcgcc
     1801 acagcggctc ttcgagccgt tccgcatgga gggcacgcgc caagcgcagg agcgcagcgg
     1861 catcggctga accgccgccc aaaccggcgg cgacgggaag gtttttctcc aacgcgatcg
     1921 ccccgcgcag gtcgaccccc tgctgccgta gcaagtcgag ggcgcgttcg aggatattcg
     1981 gagcttcgat ggcatcggca tagggcccgc tcgcgacgag gtgggtcggc tcgccgggca
     2041 cgaggctcag gcgatcggcg acagctgcaa aggtcaccag gctttccagt tcgtgatagc
     2101 cgtcgctgcg tcgccccagc accttcaggg tgaggttcac cttggcgcga gcaagttgcc
     2161 aaacgcccat gcctcgccct ccgccctccg cgcggctagc agcgctgctg gcgctccagc
     2221 gccaacggac ctacttgaac aggggcagac tgaaagtggt ttcagagctc tttttcagat
     2281 gctccttctt caccacttcc ttggttcgct tgaccggccg ggcctgctgg ggcgagggca
     2341 ggccgcgctg cagcttgcgc ttgatcttgt ccgtatcctc gggttccggc ttcagcgtca
     2401 aggcctgttc ccactggaaa cgcgcctccc gttcgcggcc gacgcgccag aacgcatcgc
     2461 ccaaatgatc attcaagacg gggtcttccg ggcgcagctc caccgcccgc tccaggtgct
     2521 tgaccgcctc tttgaagttg ccgagccggt agtgggccca gccgaggcta tcgacgatgt
     2581 agccgtcatc gcgcttctga cgcacggcct tctcgatcag cgccatgcct tgcttcaggt
     2641 tcttgccctg atcgatccat gagtagccga gatagttgag tgccagcggc tggtccggac
     2701 ttaactgcaa tgcgcgctgc aggtcggcct cggccagcgc ccatttcttg atgcgttcat
     2761 agcaggtgcc gcgcgagtag aagaaagacc aatgcttttg ctcgggcttg tcgatcagcg
     2821 caatggcgcg gctgtagtac tcgaccgcct ccgcgtaacg cttgtggccg cgcatgatgc
     2881 cgccaagggc gtcgagcggg cgcacgtcgg tgggattgcg ctgcgccgtt ccctccaaca
     2941 ggtccttggc ctcctcgacg cgctcgagct ggttgaggtt cagggccttg cgaatttcga
     3001 cgctgacctg gagcgcggta tcgttgggta tccgatcata ggcatcgatg gccgcttgat
     3061 agcgcttggt ggtctcgtag gcattggcga gggtcgccag agcgaaggga aagtcgggtc
     3121 gcaaatagag ggcaaactgc aaatatacgg cgcccacgct gacgccgcct tccccggtca
     3181 atgcctcgcc gagcccgtag aacgcctcgg caagtccttc ggtcggcgtg ccgaccagca
     3241 gcgttacctc agctccggcg tcgatttgat cctgcagtgc cttggcctcc ggatggccgt
     3301 ccgccttggc cttgtcgaag tgcgctttca aaatgcgctg ggccagcttc gcatcgccag
     3361 cgttggccgc atgacgggtg taggccagcg tgatgcgtag cgtgcgctgg tcgttggcgg
     3421 gaatgcgctc gtaggcggcg cgcgcctccg cccggcggcc ggccacgtcg gcgatcaggg
     3481 cacgatggta gcgcaggtag aactgggccc agtccggcag cttcggggca tcgagcaggg
     3541 ccagcgcctc cgtcgtcttg ccctcggcct gcgcgaccca agcccgtgcc agcgcactcg
     3601 tcaactcgcc gatcgggttg ccgctcacgg ctttgaaatg ctcgtcggct gccgcgtagc
     3661 gctgcgcctt gaagtcggcg agggccaaga gggcgtgtgc cgtgcgatgg ttgggctggg
     3721 aggcgacgag ttcacgcgcc agagcctcgg cacgggacca ttcgccctcg ctcgtctcca
     3781 tcaggaacgc ctgctcgacg agcaaattgt tgccggggtc ctgggcgagc gcctgccgat
     3841 agaacatggc cgcggcgcgg gtatcgttct gtgcccgcgc gactcgaccg gctaagtagc
     3901 tcccgagcag cgagccggct tccggtgcgg cgccgaggcc cgcgtgcgac ggcaccgcct
     3961 gggcggcaag aacggccgcc acaacagcgc tgaggaggct cagcaaagaa agccaccgac
     4021 gcatgtcacc tcgtgccgta aaagttagct ttgtgcattc gttgattccc gtgagctagg
     4081 ctagcaggaa agagttgtgc aagcgactca agaacgcccg cccaagaccg tttgttcgcc
     4141 gatcctgatc gctgccatcg gacgggcaca ttggggcaat tagaggaccg gaaagcctgg
     4201 acgcttgccg gcgggatccc tgtagtcggg gccggcaagt tgtggataat cacgcaccgc
     4261 tccggttcag gccgaaggcg gcgcccagcc gtacggcgcc ccgctcaagc gcgcgccgcg
     4321 cgtcggcgac gatacgtccc atggtgataa agccgtgtat ttgtccctcg aaccgctcca
     4381 gttcgacggg cacgtccgcc gcggcaagcg ccgcggcgaa ggcttcgcct tcatcgctca
     4441 acgggtcgaa gcccgctgtg atgatgagcg ccggaggcag gccgcgcagg tttgtcgcac
     4501 gcagcggcga ggcacgccaa tcttctatgt cttgcggtcc gttgaggtag tggttgagga
     4561 accattgcat gcctttgcgc gtcagcggca gctgctcggc gtgccgaaga atggaggcgc
     4621 tttgcatcgc catgtccgtg ccgggataga tcaaaaactg aaacaccggc cgggcctggc
     4681 cgctatcgcg cgccgcaatg gctgcgacgg ccgaaagatt gcccccggca ctgtccccgg
     4741 ccaccgccag gcggctagga tcgaccttga gccggtcggc attggccgca atccagcgcg
     4801 tggcggcgat cgcatcctcc acggcggcgg gaaacttgtg ctcaggcgca agccgatagt
     4861 gcacggctat cacagtcgcc ggcaccgcgt ttgcgagtgc gcggcagagc tggtcgtggc
     4921 tttccaaatc gccgatcacc catcccccac cgtggaagta gacgaccacg ggctgcgggc
     4981 ttgccgcttc ggccggccgc ccgcgataga gtcgcaaggg gattggtccc gctggcccct
     5041 ctgcctgcag gtctgtcatc tccgcgaccg gttcgggatc gggttgcagg atcggcttgg
     5101 aggcgaggta aacggcgcgc gcctcgaccg gcgttagcgt ctcgatgggc gggcgacccg
     5161 aggcgatgat catctcgcac accctttgtg cgtcgggatg tagcttgtcg gcgggcattg
     5221 ctccctcctt gcgtggttag ctattcgagc gggcgctcac atgccggaat aattcggtcc
     5281 gccgccgccc tccggcacca cccaatcgat gttctggttg ggatccttga tgtcgcaggt
     5341 tttgcagtgg acgcagtttt gcgcattgat ctggaagcgg agcttgcccg cctcctcgag
     5401 ccattcgtag accccggcgg ggcaatagcg cgtcgagggg cccgcataaa catcgtgctc
     5461 ggattgccgc tgtaggacgg gatcgcgcac cttgaggtgt acgggctgat cctcctcgtg
     5521 attggtcgcg gagaaggaga cgttggtgag gcggtcgaag gtcaacgtgc catcgggcct
     5581 gggataatcg agcgggcgct cggccttggc gatatcgagc gtcgcatgat ccggtttgcc
     5641 gtgcttgagg gtgtagccaa agccaaggcc gggaaggacg gtgttggccc acatatccgc
     5701 gccggagagc gcggtgccga gaagcgtgcc gaacttagac agcatcggct tggcgttgcg
     5761 cacgcgccgc aaatcgcggg tgatcggccc cgaagctacc tcggccgcgt aggcctcgag
     5821 ccgatcctgc gcgcgacccg cggcaatcgc cgcaaaggcg gcgtctgccg cggcaatgcc
     5881 ggacagcatg gcattgtgcg agcccttgat tcgcggcacg ttcatgaagc cggcggcaca
     5941 gccgatcagc acgccgccgg gaaacgtaag ccgcgggatc gactgcaggc cgccctcggt
     6001 tagggcgcgg gcaccgtaac cgatgcggcg ccccccctcg aaggtaaccg agatcagcgg
     6061 gtgcgtcttg aagcgctgga attcttgata gggcgaaagg taggggttgg cgtagttgag
     6121 gtgcacgaca aaaccgaccg acaccaggtt ggcgccgtag tgatagagga aagagccacc
     6181 ccctgtgcgg tcgtccaagg gccaaccgag tgtgtgctgc accaggcctg gacggtggcg
     6241 ctcgggtgcc acctcccaca attctttgag acccaagccg aacttctggg gttcgcgacc
     6301 ctcgcgcagg ccgaacttgg ccgtcagcac cttggtcagg gagccgcgcg ccccttcggc
     6361 gatgatggtg tacttgccct tgagctccat accccggctg aatcgctccg tcggcttggc
     6421 atccttgtcg agccccatgt cgccggtggc aacgccggta acggcgccgt tctcgtcata
     6481 gagaacctcc gcggccgcga agccgggata aatttcgacg ccgagctccg ttgcctgctc
     6541 gccgagccag cggcaaagat tgcccaggct gacaatgtag ttgccgtggt tgcgcatgag
     6601 cggtggcatg atcaggttcg gtatgcgcaa ttggcccgcc ggtccgaggt aatagaagcg
     6661 atcgtcggtc acctccgttt cgacgggggc acccttggct cgccaatcgg gaatgagcgc
     6721 attgaggcca atcgggtcga tcacggcacc cgacagaata tgcgccccga cctcggagcc
     6781 cttttccaag acgaccacag agagctcgcg atcgcgctct gcggcgagtt gcttgagccg
     6841 gatggcggcg gcaaggcccg cgggacccgc gccgacaata acgacgtcga actccatcgc
     6901 ctccctgggc gagctctgag catccgcaag cgccatgggc gtgttcccga agtgatcccg
     6961 tgtggttcga taggtcgagc ccgcccggcg cgtcaaggtc gcgctcgcgg agaacggggc
     7021 tttgctgcaa ttcgcgcgcg gctggcatag attgccggca tgaccgatca gcgtcacgcc
     7081 gacctggccg ccctgttggg ctggtaccgc gccatgggcg ctgacgaggc gctcagccag
     7141 agcccgatcg actggcttgc tcgcggcgag aagggcccag ggtggtgctt cgccctgacg
     7201 gctccggcaa agccgccggc tgcgtcggga gcgccggtga gcacgccgcc gccgcggccc
     7261 cacgccgcca cgcccgtcct gccgccgggc gccccgcagc ggccgtttgc
//
LOCUS       DASTMT010000261         4611 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_3420355, whole genome shotgun sequence.
ACCESSION   DASTMT010000261 DASTMT010000000
VERSION     DASTMT010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4611
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3420355"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..353)
                     /locus_tag="VFR00_06100"
     CDS             complement(<1..353)
                     /locus_tag="VFR00_06100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0158861.1"
                     /translation="MRAKLRHLAANLVLLAASLFVSLLFVELVVFGMFLKPDDVLANV
                     SLNGVVRYQPNTRATFRHPDGHETHVSINAQGWNSTKADYVPARPAGRLRIAVIGDSY
                     VHGSFINVEEGFPEVV"
     gene            complement(480..878)
                     /locus_tag="VFR00_06105"
     CDS             complement(480..878)
                     /locus_tag="VFR00_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009736265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2306 domain-containing protein"
                     /protein_id="HEU0158862.1"
                     /translation="MSLTPLLQAPLAIQIHAFAAFAAFLLGLVQFARLKGTHTHRLIG
                     YTWVGLMLFVAVSSFFIQELRHWGSFSFIHLLSILTLAMLPLGIYFARRHNVVGHRKT
                     MLGLFFGALVVAGAFTLLPSRILGRVVFGG"
     gene            complement(947..1444)
                     /locus_tag="VFR00_06110"
     CDS             complement(947..1444)
                     /locus_tag="VFR00_06110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018242013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEU0158863.1"
                     /translation="MAGSVNKVILIGNLGKDPEIRRTQDGRPIANLRVATTETWRDKA
                     SGERREKTEWHSVVIFNEGLCRVAEQYLKKGAKVYIEGQLQTRKWQGQDGQDRYSTEV
                     VLQGFNSTLTMLDSRGGGASLGMQDDEHGDYGAPSNGNGGSGRRGGGGSKANFDKPID
                     DEIPF"
     gene            2060..2731
                     /locus_tag="VFR00_06115"
     CDS             2060..2731
                     /locus_tag="VFR00_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013419249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0158864.1"
                     /translation="MTQLKRLKGIVCLAAAAGGLALLAGCTHERLGPVSQHYIDFHTE
                     APRKNTVYVCHAYGCRMQTAFRFTDADIAQLKALMTKTRKADTPFEERRAVAYAIGWM
                     ETRTGDVIGTSADRPGMDFAASGDPTQQDCVDEATNTTSYLLILERNGLLKHHTVGTP
                     FSKENLLRGVAGWPHWTAVLKENANGQKWAVDSWIYANGENPAIVEAEKWYISSLDQL
                     PKSTH"
     gene            2813..3943
                     /locus_tag="VFR00_06120"
     CDS             2813..3943
                     /locus_tag="VFR00_06120"
                     /inference="COORDINATES: protein motif:HMM:NF021446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2157 domain-containing protein"
                     /protein_id="HEU0158865.1"
                     /translation="MSWYRTKLERDLARWQTEGWVSPAAAGAIRRDLDQRKSLFSAGP
                     VLAVLGAVLFGFAIMSFVAAHWTGMSKLARLALLLAALWGCYGGAAVLFQRQLNAFAQ
                     AAVLAGIAVFGASIMLIAQMYHMEGHPADAILLWALGALLAAVLTGSTPALAATFVLL
                     SVWGIWERLDSESAHFMFLLPWGGAALTAAWLGWRPGFHLAALSLLLWIVPLGFLIWG
                     YHAHWLVVLIGAAVAVGAAAAAESLDRHLPISGALFAYGLLIAYAGLFILQFLDDSRW
                     LVSSSQGPEGAGIARLLLLAIVSLVGLLGAMLFALRTDNRPALWLAYAAFALEIFSLY
                     VKTFGSFLNTSLFFLVAALIVSGLAWAAYRLHQSKSATGAGA"
     gene            3940..4521
                     /locus_tag="VFR00_06125"
     CDS             3940..4521
                     /locus_tag="VFR00_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDYXXLXY domain-containing protein"
                     /protein_id="HEU0158866.1"
                     /translation="MRLFATRIAYALAIVALLQTAALAVMVFNRVRLLQTGREITLPI
                     VPVDPRDLFRGEYVELGYAVGRVPARLLDGPAPLGNAPFYVTLEKAQDGSWAPVKVSR
                     DRPQETSPERIVLKGRSRFGLFTSDGNNATAINNVRYGIEQYFVPQGEGPRLEALARD
                     KKLAALIAVDASGNAAIKGLLIDGQLQYEEPLL"
BASE COUNT          783 a         1528 c         1473 g          827 t
ORIGIN      
        1 acgacctcgg ggaagccctc ctccacgttg ataaaactgc cgtgcacgta agaatcgccg
       61 atgacggcga tccggaggcg cccggcgggc cgcgccggga cgtagtccgc cttcgtcgag
      121 ttccagcctt gggcgttgat gctgacgtgg gtttcatgcc cgtcggggtg gcgaaaggtg
      181 gcccgcgtgt tgggctgata gcgcacgacg ccgttcagcg agacgttcgc cagcacgtcg
      241 tcgggcttga ggaacatccc gaacacgacg agttcgacaa acagcagcga cacgaacagg
      301 gaagccgcaa gcagcaccaa attggccgcc agatgtcgca atttggcgcg catcgtcctc
      361 ctgccccgca gcgtcaggcc ggtccggcca gccgcaccgt caacaacgca aggcccatgc
      421 cagagaaggg gccagcggag gggccaaaca aggggggtaa gagggcgagc cgcgaacgct
      481 caaccgccga acaccacgcg gcccaaaatg cggctgggca gcagcgtgaa agcgccggca
      541 acgacgagag cgccaaagaa cagtccgagc atggtcttgc gatggccgac gacgttgtgg
      601 cggcgggcaa agtaaatgcc gagcggcagc atggccagcg tcaaaatcga caacagatgg
      661 atgaaactaa agctccccca gtgccgcaat tcctggatga aaaaagagct caccgccacg
      721 aacagcatta agccgaccca ggtgtagccg atcagccggt gtgtgtgcgt ccccttgagg
      781 cgggcgaatt ggacgaggcc caagaggaag gcggcgaacg cggcgaaggc atggatttga
      841 atggcgagtg gcgcctgcaa gaggggggtg agcgaaatgg gtcaaccctc caaggtgagc
      901 cggcaggcgc tagcggtcgc cggcgccagc cgcccgcgtg caccggctag aaggggattt
      961 cgtcgtcgat aggcttgtcg aaattggcct tggacccgcc gccgccacgc cggccgctcc
     1021 ccccattgcc gttgcttggg gcgccatagt cgccgtgctc gtcgtcctgc atgccaaggc
     1081 tggcgccacc gccccgactg tcgagcatcg tcagcgtgct gttgaagccc tgcagcacga
     1141 cctcggtgga gtagcggtcc tggccgtcct gtccctgcca tttgcgcgtc tgcagctgac
     1201 cctcgatgta cactttggcg cccttcttca ggtactgctc ggcgacccgg cagagcccct
     1261 cgttgaagat caccacggag tgccactccg tcttttcgcg ccgctcgccg ctggccttgt
     1321 cgcgccaggt ctcggtggtc gcaacgcgca ggttggcgat cggacggccg tcctgcgtgc
     1381 ggcggatctc gggatccttg ccgagattgc cgatcaggat gactttgttg acggaaccgg
     1441 ccatgaaacg ctccttctca aacggttctt cgggtctgcc gtgctcggtg tgccgctccc
     1501 gggcttgtcc gagcctcctg gcctggcgcc gacctgaggg ctgcgcaacg cacccaggct
     1561 ggccccgtga ttcgtgacac gacgatagcg gctctcgacc atctccgggc gtcgcccaag
     1621 ttgcttgtcc acgctattcg acgcggccgc tcggtccggg gcggacaggc ccccttccaa
     1681 gatcgacatg cgtctaaggt cggcatggcg ctccggtttg ccaagccgcc ccgcccacac
     1741 atcctcggcc ggtctgccaa gcccgccgtc tccaacctcg ctgcagccta aacaacctgt
     1801 gcccatgcac ggctatgatg ttctcgttat gttcttatgt agcgcagaac cccgcccggc
     1861 gcaagggcgt gcggcggctg ccctcctgtt cacgatcgag ggtggcgcac ccatcgcctc
     1921 gcgcggcttg gacgcgctct ggtaagccgc cgtcttgcgg tccaatcacg cgcgctttgc
     1981 cacgaatcac aagagtgata gccgtcagtc gggaaggccg ccctcagcgg tcccgagtcg
     2041 atggcgacgc tggcgggcaa tgacgcagct caagcggttg aaggggatcg tttgtttggc
     2101 tgcggctgcc ggcggcctgg cgctgcttgc aggttgcacg cacgagcgtc tcggtcccgt
     2161 gagccaacat tacatcgact ttcacaccga ggccccgcgc aaaaacacgg tctacgtctg
     2221 ccatgcctac ggctgccgga tgcaaacggc gtttcgcttc accgatgccg acattgccca
     2281 actcaaggcc ttgatgacca agacgcgcaa ggccgacacg ccgttcgagg agcggcgcgc
     2341 ggttgcctac gccatcggct ggatggaaac gcgcacgggc gatgtgatcg gcaccagcgc
     2401 cgaccgcccg ggcatggatt tcgctgccag tggtgacccg acccagcagg actgcgtcga
     2461 cgaagccacc aatacgacca gctacctctt gatcttggag cgcaatggtc tgctcaagca
     2521 ccacaccgtc ggcaccccct tttccaagga gaacctgttg cgaggggtgg ccggctggcc
     2581 gcactggacg gccgttctca aggaaaatgc caacggccag aaatgggctg tcgacagctg
     2641 gatctacgcc aacggcgaga acccggcgat cgtggaagcc gagaaatggt acatctcgag
     2701 cctcgatcaa ctgccgaagt cgacgcacta gccgcgcagc agccaagact tgacagcccg
     2761 ccttgtgcgc ccatgctacg ggcaaccccc ggggtgggag cgccacaggt aaatgtcctg
     2821 gtatcgcacg aagctcgagc gcgatttggc gcgctggcaa accgagggtt gggttagtcc
     2881 cgccgccgcc ggcgccattc gtcgcgatct cgatcagcgc aaatccctct tcagcgccgg
     2941 tcccgtgttg gcggtgttgg gcgccgtcct gtttggcttc gcgatcatga gctttgtggc
     3001 cgcgcactgg accgggatga gcaagctcgc ccggctcgca ctgctgctgg ccgcgctgtg
     3061 gggctgctat ggcggcgcgg ccgtcttgtt tcagcgccag ctcaacgcct ttgcccaggc
     3121 ggcggttctg gccggtattg ccgtcttcgg cgccagcatc atgttgatcg cgcagatgta
     3181 ccatatggaa ggccatcccg ccgatgccat cttgctatgg gcgctcggcg cgctgctggc
     3241 cgcggtgctg acgggctcca cgccggcctt ggcggcgacc ttcgtgctct tgagcgtgtg
     3301 gggcatctgg gaacgcctcg acagcgagag cgcacacttc atgtttctgc tgccttgggg
     3361 cggggcggca ctgacggcgg cgtggctcgg gtggcgtcca ggcttccacc tcgccgcgct
     3421 cagtttgctg ctgtggatcg tacccctggg cttcctgatc tggggctacc acgcccattg
     3481 gctggtggtg ctgatcggtg ccgccgtcgc ggtcggtgcg gccgccgcgg ctgagagcct
     3541 ggaccggcac ctgccgatct cgggcgcgct cttcgcctac ggtctcctca tcgcctatgc
     3601 aggcctgttc atcctccagt tcctcgacga cagccgctgg ctggtgagca gctcccaggg
     3661 tcccgagggc gccggcatcg cccggctgct cctgctggcc atcgtgtcgc tcgttggact
     3721 gctgggcgcc atgctgtttg cgctcaggac ggacaatcgg ccagccctgt ggcttgccta
     3781 tgccgccttc gcgctcgaaa tcttctcgct ctatgtcaaa acctttggct cgtttctcaa
     3841 tacctcgctg tttttcctgg tcgcggccct gatcgtcagt gggctcgcct gggccgccta
     3901 ccggctgcac caatcgaaaa gcgcgaccgg agccggcgca tgaggctttt tgcgacccgc
     3961 atcgcgtacg ccctcgccat cgttgcgttg ctgcaaacgg ccgcgctcgc cgtcatggtc
     4021 ttcaaccgcg tacggctgtt gcagaccggc cgcgaaatta cgctcccgat cgtgcccgtc
     4081 gatcccagag accttttccg cggcgaatac gtggagctcg gttacgccgt cggacgcgtg
     4141 ccggcacgcc tcctcgacgg gccggcaccc ctgggaaatg cccccttcta cgtcaccctg
     4201 gaaaaagctc aggacggcag ctgggcgccg gtcaaggtca gccgcgacag gccgcaggag
     4261 acatcccctg agcgcatcgt gctcaagggc cgcagccgtt ttgggctgtt cacaagcgat
     4321 ggcaacaatg ccaccgccat caacaacgtg cgctacggta tcgaacagta cttcgtgccg
     4381 cagggcgagg gtccacgcct ggaggcgctg gcccgcgaca agaagctcgc cgccctgatc
     4441 gccgtcgacg ccagcggcaa tgcggccatc aagggcctcc tcatcgatgg gcagctgcag
     4501 tacgaggagc cgcttttgtg agttggcgcg cgcaagcggg cgcgtgctaa gacgtctcga
     4561 cgagcagcca acagggagag tgccatggcg atcacgcgtc acgaaccgtc t
//
LOCUS       DASTMT010000262         1609 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_2434253, whole genome shotgun sequence.
ACCESSION   DASTMT010000262 DASTMT010000000
VERSION     DASTMT010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 1609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1609
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2434253"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1136)
                     /gene="aroA"
                     /locus_tag="VFR00_06130"
     CDS             complement(<1..1136)
                     /gene="aroA"
                     /locus_tag="VFR00_06130"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEU0158867.1"
                     /translation="MLQTANSPLISLRSGPLSGSVRVPGDKSISHRALMLGALASGRT
                     RIAGLLEGEDVLNTGRVLAAMGCPVERDGGGVWEVLGRGVGGLAQPAVPLDFGNSGTG
                     VRLMMGLLAGNDLLVRLVGDASLSRRPMGRVLDPLVRMGVEVEDGARTLPLSLRGTSD
                     LLPIEYQLPVASAQIKSAILLAGLHASGATSVIEPEATRDHTERMLRYFGGEVTVGSR
                     AGKRLITVAGNAELEGRSVEVPGDPSSAAFLTAAALMVPQSQIRIEGVLINPTRTGFY
                     TTVREMGADLVFLNEREQGGEPVADISVRHSKLKGVRVPPGRAPSMIDEYPILACLAA
                     YASGETHMAGLAELRVKESDRLAATVAGLQANGVSASAEGDLLCV"
     gene            1351..>1609
                     /locus_tag="VFR00_06135"
     CDS             1351..>1609
                     /locus_tag="VFR00_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02300 family protein"
                     /protein_id="HEU0158868.1"
                     /translation="MSTKAERGTKRTCQNPECGSRFYDLNRDPIVCPICQSSYEIVAS
                     SVAMAAAAPMADTPRRPAKKPAYEVAGIKPEDVPEAESDELA"
BASE COUNT          285 a          564 c          496 g          264 t
ORIGIN      
        1 acgcacagaa gatctccctc cgcgctggca ctgacgccgt tcgcctggag gccggccacg
       61 gtggccgcca ggcggtcgct ctccttcacc ctgagctcgg caagcccggc catgtgggtt
      121 tcgccgctcg cgtaggcggc caggcaggca aggattggat actcatcgat catgctgggg
      181 gcgcgtcccg gcggcacgcg cacgcccttg agtttgctgt ggcgcacgct gatgtcagcg
      241 accggctcgc cgccttgttc gcgctcgttc aggaagacga ggtcggcccc catctcccgc
      301 actgtcgtgt agaagccggt gcgggtaggg ttgatgagca cgccttcaat gcggatctgc
      361 gactgtggca ccatcagcgc ggcggcggtc aggaaggcgg ccgaacttgg atcgcccggc
      421 acctcgacgc tgcgcccctc cagttcggca ttgcctgcca cagttatcag ccgcttgccc
      481 gcgcgggacc cgaccgtcac ctcgccgccg aagtagcgca gcatacgttc ggtatgatcg
      541 cgcgtggcct ccggttcgat cacgctggtt gcgccggagg cgtgcagccc cgccagcaaa
      601 atggccgact tgatctgggc agaggccacc ggcagctggt attcgatcgg cagcaaatcg
      661 ctggtgccgc gcaaagacag cggcaaagtc cgtgcgccat cctctacttc cacgcccata
      721 cgcacgagcg ggtcgagtac gcgtcccatt ggtcggcgcg acagcgaggc gtcgccgacc
      781 agccgtacca ggagatcatt gccggcgagc aaacccatca tcagccgcac gccggtaccc
      841 gaattgccaa aatcgagcgg caccgccggc tgggccagtc cgccaacgcc ccgtcccagc
      901 acctcccaga cgccgccccc gtcccgctct accgggcagc ccatggcggc cagcacgcgc
      961 cccgtattga gcacgtcctc tccctccaag agcccggcga tgcgggtgcg tccgctcgcc
     1021 agggcgccca gcatcagcgc gcgatgggag atcgacttgt cccccggcac gcgaacgctg
     1081 ccgctcaggg ggcctgagcg cagggaaatt aggggtgaat tagccgtttg cagcatgtcg
     1141 gcggcagcct taaacggggg tcctcgttgg cgcttgccgg tggaccccgg cgccccgtca
     1201 tagagcagcg gcttgccccc ctgtcaacaa tcggcaaagc ccctatatga aggccagaaa
     1261 atcttgcctt tgtccggccg atttggtttt gacaccccag ccggcgcgtg gcaatgaacc
     1321 caccaatccg acagtttaga ggcctttttc atgtcgacga aagccgagcg cggcaccaag
     1381 cgtacgtgcc aaaaccccga atgcggctcg cggttttacg atctcaatcg cgaccccatc
     1441 gtttgtccga tctgccaatc gagctatgaa atcgtggcat cctcggtggc catggccgcc
     1501 gccgcgccaa tggcagacac accccgccgg ccggccaaaa agcctgccta tgaggtggcc
     1561 ggcatcaagc ccgaagacgt ccccgaggcg gaaagtgacg agcttgccg
//
LOCUS       DASTMT010000263         1550 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_3094385, whole genome shotgun sequence.
ACCESSION   DASTMT010000263 DASTMT010000000
VERSION     DASTMT010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 1550)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1550)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1550
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3094385"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            287..>1550
                     /locus_tag="VFR00_06140"
     CDS             287..>1550
                     /locus_tag="VFR00_06140"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEU0158869.1"
                     /translation="MHTAVRIGWTMLVLLLAPDARAQQQNILLFVADGLRAAAVSPET
                     APTMARLRDQGVNFRNSHSLFPTVTTANASAIATGHYLGDTGDFGNSLYSGFPVASVE
                     GVLTPFIQSNAVLAELNRHTGGNFLAERSLMAAARERGYLTAVLGKVGPVAIQDITEL
                     EANTTIFFDDDTGKRSGIALRPDIVELLQKAGLALETPGRAQRTNPGKSTNIVQQTYY
                     RDIVTKVILPLSKATGKPFMVVYWSPDPDGTQHSQLDSENTLTPGIDGPTSLAAIKVA
                     DDDLAAILATLQDLGLATKTNVFVTADHGFSTVSKASTTSPSVRMSYAGVPPGQLPPG
                     FLAIDLATVLDLPLHEPFAKGPAVDFKAGKFPRRANAVLGQDPGKPDIVVAANGGADL
                     IYLPQDNAKTLAPKIVKALFAQDYVSGVF"
BASE COUNT          273 a          568 c          448 g          261 t
ORIGIN      
        1 cgcttgagcg gcgggccgat cctctcgccc ggccagagct cctcatggcc gtaatgcgcg
       61 ccggcatcga aggcaccgta ggcggctctg tcggttgcag gctgagcggg ggctctcatc
      121 tccgaccctc gtgtcccgtg tggataggtc aggctcccgc gctgcctacg cgccagcaaa
      181 ttcctggcaa aataaaggca acggcccaac aaacccggcc gttgccgccg gccggttccg
      241 acgcctaatc tggcgatgtg gccgcgggag accgggggga agcctcatgc atactgctgt
      301 tcgcatcggc tggacgatgc tcgtcctgct cttggcgccg gacgcacggg cccagcaaca
      361 gaacatcttg ttgttcgtcg ccgatgggct gcgcgccgcg gcggtctcgc ccgagacggc
      421 gcccaccatg gcacgcctgc gcgatcaggg cgtcaatttc cgcaacagcc actcgctgtt
      481 ccccacggtc accacggcca acgcctcggc gatcgccacc ggccattacc tcggcgacac
      541 cggcgatttc ggcaattccc tctactcggg ttttccggtc gccagcgtgg agggtgtcct
      601 tacgcccttc atccaaagca atgcggtcct ggccgagctc aatcgccata ccggcggcaa
      661 ttttctggcc gagcgctcgt tgatggccgc cgcccgcgag cgcggctatt tgaccgcggt
      721 gctcggcaag gtcggccccg tcgccatcca ggacatcact gagctcgaag ccaataccac
      781 gatcttcttc gacgacgaca ccggcaagcg cagcggcatc gcgctccgcc ccgacatcgt
      841 ggagctgctc cagaaagccg gccttgccct cgagacgccc ggccgcgccc agcgcaccaa
      901 tcctggcaaa tcgaccaata tcgtgcagca gacctactat cgcgacatcg tcaccaaggt
      961 gatcctgccg ctcagcaagg ccacgggcaa gccgttcatg gtggtctact ggtcgcccga
     1021 ccccgacggc acccagcact cgcaactgga cagcgaaaac acgctgacac cgggcatcga
     1081 cgggccgacc tcgctcgccg ccatcaaggt cgccgacgac gatctggccg ccatcctcgc
     1141 cactctccag gatctcggcc tcgccaccaa gaccaacgtg ttcgtcactg ccgatcacgg
     1201 cttcagcacc gtctccaagg cgagcaccac cagtccgtcc gtgcgcatga gctatgcggg
     1261 cgtgccgccc ggacaattgc cgccgggctt ccttgcgatc gatcttgcaa ccgtcctcga
     1321 cctgccgctg cacgagccct tcgccaaagg tcccgccgtc gatttcaagg ccggcaagtt
     1381 cccgcggcgc gccaacgccg tgctcggcca agaccccggc aagcccgaca tcgtggtggc
     1441 cgcgaacggg ggtgccgatt tgatttacct gccgcaagac aatgccaaga cgctggcgcc
     1501 gaaaatcgtc aaggctctat tcgcccagga ttatgtcagc ggcgtcttcg
//
LOCUS       DASTMT010000264         3350 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_139632, whole genome shotgun sequence.
ACCESSION   DASTMT010000264 DASTMT010000000
VERSION     DASTMT010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3350)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3350
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_139632"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..745
                     /locus_tag="VFR00_06145"
     CDS             <1..745
                     /locus_tag="VFR00_06145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0158870.1"
                     /translation="EGHSYNNNYLTDSGGRCLAPAPTCVQMSRRGRCTAWSAPAISAA
                     QAQALTCKYDGGSIGAGPGPNALCDTQPILPLTENKGAVDAAIGQMRAKGSSNILEGL
                     MWGWRVLSPEAPFTEGRPYSDPENRKYLILMTDGGNEHQAVANHNRSIYHAFGYAANG
                     RLGNTATTSALISQLNSKTRTACENTKAAGITVYTIALRLESDANTRALLASCASSAA
                     EAYRASDGATLVQAFEAIAREIAKLRIAS"
     gene            1038..1769
                     /locus_tag="VFR00_06150"
     CDS             1038..1769
                     /locus_tag="VFR00_06150"
                     /inference="COORDINATES: protein motif:HMM:NF021025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaD family pilus assembly lipoprotein"
                     /protein_id="HEU0158871.1"
                     /translation="MSQRAQPAAVKSGSRGGAVAAALLALVLAACDHYHWWETRQLDN
                     ATALGLSEPQVPHPMPFAARRETLDIEVPPDAEGLSPDQHIDVVRFLEEQTHEAKRRL
                     VVFVPTPQRSAGAIARTLQDIQRHIVDAQVTYRVVKMTAWERGHTALASIRLAYERPV
                     AVAPTCGDWSEDVGRKAERVADPNGDCATQRPWTVLVDPAREEPRASEKRAQTWSTSM
                     STAAKSGAVDARAPGKTGAPSAAKP"
     gene            1858..2463
                     /locus_tag="VFR00_06155"
     CDS             1858..2463
                     /locus_tag="VFR00_06155"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011580204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEU0158872.1"
                     /translation="MTFKLPELPYAYDALSPYMSKETLEFHHDKHHQAYVDNGNKVAG
                     AGSKYDGKSIEDICREAFAAKDAPVINNIGQHYNHIHFWQWMKKGGGGKKLPGKLQTM
                     VDKDLGGFDKMRTDFVQAGVSQFGSGWAWLAVKNGKLEVAKTPNGENPLMHGASPILG
                     VDVWEHSYYIDYRNARPKYLEAFFDHLINWEHVEAMLEKAK"
     gene            complement(2477..2890)
                     /locus_tag="VFR00_06160"
     CDS             complement(2477..2890)
                     /locus_tag="VFR00_06160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0158873.1"
                     /translation="MTIRLRLGAIPVCVLLWATPAAAAMASVDCRAPRMAPADLTICT
                     DPQLSRLDDLLSRRLARTARQLAYGPYVGLRVWQSDWRQQRNECSADRACLAAVYNDA
                     NRFLDRFQRCLGTSLRGRRCLPVSVEGERAAVRRP"
     gene            complement(3104..>3350)
                     /locus_tag="VFR00_06165"
     CDS             complement(3104..>3350)
                     /locus_tag="VFR00_06165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0158874.1"
                     /translation="SGAGEAWPVGRAGAAPMRSEDAVPAAAASAPGKAEAAQARARAA
                     LAAERAAVEPATAWAGEGGPLGSKVGPEAAAVCIAGC"
BASE COUNT          626 a         1185 c         1072 g          467 t
ORIGIN      
        1 cgagggccac agctacaaca acaattacct caccgacagc ggcgggcgtt gccttgcccc
       61 ggcacccacc tgcgtgcaaa tgagccgccg cggccgctgc accgcctggt cggcgcccgc
      121 catttcggcg gcgcaagccc aggcgctcac ctgcaaatac gacggcggct ccatcggtgc
      181 cgggccgggt cccaacgcgc tctgcgacac acaaccgatt ttgccgttga ccgagaacaa
      241 aggtgccgtc gacgccgcga tcgggcaaat gcgcgccaag ggcagcagca acattctcga
      301 gggtctgatg tggggctggc gcgtgctctc gcccgaggcg cccttcaccg aggggcgtcc
      361 ctatagcgac cccgaaaata ggaaatacct gatcctgatg accgacggcg gaaacgagca
      421 ccaggccgtg gccaatcaca accgctcgat ctatcacgcc tttggttatg ccgcgaacgg
      481 caggctcggc aacaccgcca cgacctcggc tctcatcagc caattgaaca gcaagacacg
      541 gaccgcttgc gaaaacacca aggccgcggg catcaccgtc tataccatcg ccttgcgcct
      601 cgagagcgac gccaacacgc gcgcactgct ggcaagctgt gcctccagtg ccgcggaggc
      661 ctatcgcgcc agcgacgggg cgaccctggt gcaagccttc gaagcgatcg cccgcgagat
      721 cgccaaactg cgaatcgcca gttgaggcct gccaaccgcc gtcgcatcgg caagacgcgc
      781 cgtttgtgca cagctaattg cccaagggct tggcggggcg cccaccgtgc cgctgccgcc
      841 gcgccacacg ccacaaagct gcgcacggcc tgcgaacaaa ctgtcgatgg gctggggaga
      901 agctgggatt gagccgcgcg gcgctagagc tcgcagcgcc aatgccgcaa aaggacaaag
      961 ggcgtttgtg cctgtcgacg ggcgcggaca gcacggcgtc aatcgggcct cgacgatccg
     1021 cacgcggggg tgaagggatg agccagcgcg cacagcctgc ggcagtgaag tccggcagca
     1081 gagggggcgc cgtcgccgcc gcgctgctgg cgctcgtgct ggcggcctgc gaccactatc
     1141 actggtggga gacgcgccag ctcgataatg ccaccgcgct cgggctcagc gagccgcagg
     1201 tgcctcatcc catgccgttc gccgcccggc gcgagacgct cgacatcgaa gtgccgcccg
     1261 acgccgaggg gctgagcccc gatcagcaca tcgacgtggt gcgctttctc gaagagcaaa
     1321 cacacgaggc caagcgccgg ctcgtcgtgt tcgtgccaac cccgcagcgc agcgccggcg
     1381 ccatcgcccg caccctgcag gacatccagc gccacattgt cgatgcccaa gtcacctatc
     1441 gcgtcgtcaa gatgacggcc tgggagcgtg gccacaccgc gcttgcgagc attcggctgg
     1501 cctacgagcg gccggttgcc gttgccccga cctgcggcga ttggagcgaa gatgtggggc
     1561 gcaaagcaga acgcgttgcc gatccgaacg gggattgcgc cacacaacgc ccctggacgg
     1621 tgctggtcga cccggcgcgc gaggagccgc gggccagcga aaagcgggcg cagacctggt
     1681 ccacttcgat gagcacggcg gctaagtcgg gggcggtcga cgcccgcgcg ccgggcaaga
     1741 ccggcgcccc cagcgctgcc aagccctaac agccgcgcgc ttgtcttcac ggccccgcgt
     1801 caggtacgct cgcagcaagc cctaaccccc acacccccag cgcaacggag tatcgccgtg
     1861 acgttcaagc tcccagaact cccttatgcc tatgacgcgc tcagccccta catgtccaaa
     1921 gagacgcttg agtttcatca cgacaagcac catcaggcgt acgtcgataa cggcaataaa
     1981 gtcgctgggg ccggcagcaa atacgacggc aagagcatcg aagacatctg ccgggaggcc
     2041 ttcgcggcca aggacgcccc cgtcatcaac aacatcgggc agcattacaa tcatatccac
     2101 ttctggcagt ggatgaagaa gggcgggggt ggcaagaagc tgccgggcaa gctgcagacc
     2161 atggtcgaca aggaccttgg cggcttcgac aaaatgcgca cggacttcgt gcaggcgggg
     2221 gtgtcccaat tcggctcggg ttgggcctgg ctcgccgtca agaacggcaa gctcgaggtc
     2281 gccaagacgc cgaatggcga gaaccccttg atgcacggcg cctcgccgat cctgggcgtc
     2341 gatgtgtggg aacacagcta ttacatcgac tatcgcaacg cgcgcccgaa atatctggag
     2401 gcctttttcg accatctcat caattgggag catgtcgagg ccatgctcga gaaggcgaag
     2461 tgacgagcgc caggccttag gggcggcgga ccgccgcccg ctcgccctca acgctcaccg
     2521 gtaggcaacg gcgcccgcgc aggctagtgc cgaggcaacg ctgaaaacgg tcgaggaagc
     2581 ggttggcatc gttgtagacg gcggcgaggc aggcgcggtc ggcgctgcac tcgttgcgct
     2641 gctggcgcca atcgctttgc cacacgcgca agccgacgta gggcccatag gccagctggc
     2701 gagccgttcg tgccagccgt cggctcaaca ggtcgtcgag gcgcgacagc tgtgggtcgg
     2761 tacaaatcgt caggtccgcc ggcgccatgc gcggcgcccg gcagtcgaca ctcgccatcg
     2821 ctgccgccgc gggggtcgcc cacagcaaca cgcagaccgg gattgcgcca agacggagtc
     2881 taatcgtcat tctcgccgtg ccgaaagctg aaacggcctc ccccatctgt gcctgctccc
     2941 gcggctttgg tccagcccct tccttgcgcc gaccggcaac ggccaatcta accttaaaaa
     3001 taggcaccga ccctccagga catgcgccga accgggccaa agcctggggc gcctgaggcg
     3061 ccgcgccgcc ggcgggtatc ctcctcggcc tccaagcgcc ggctcagcag ccggcgatgc
     3121 agacggccgc ggcctctggg ccaaccttgc tgccgagcgg ccctccctcc cctgcccacg
     3181 ccgtggccgg ctcgaccgcc gctcgctccg cggccagggc ggcgcgcgcc cgcgcttggg
     3241 ccgcctccgc cttgcccggc gccgaggccg cggcggcggg caccgcgtct tcgctgcgca
     3301 tcggcgcagc gccggcgcgg cccaccggcc aggcctcgcc tgccccagaa
//
LOCUS       DASTMT010000265         4393 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_4666977, whole genome shotgun sequence.
ACCESSION   DASTMT010000265 DASTMT010000000
VERSION     DASTMT010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4393)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4393)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4393
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4666977"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..521
                     /locus_tag="VFR00_06170"
     CDS             <1..521
                     /locus_tag="VFR00_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019902977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HEU0158875.1"
                     /translation="ALFLSEMLTASAMQAYQGEIERAVVLTLFIPLIMSSGGNSGSQA
                     TSLVIRALALREIGLADWWRIASRELPAGLTLGTILGVLGVARIVLWQHLGLYDYGEH
                     WGLVALTVGLALTAIVTFGSLTGSMLPFALKRLGFDPATASAPFVATLVDVSGLVIYF
                     SVAYMVLRGTLL"
     gene            619..1491
                     /locus_tag="VFR00_06175"
     CDS             619..1491
                     /locus_tag="VFR00_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018262001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU0158876.1"
                     /translation="MTTALDVSHHFADLGEVLMHYVTAGEGPPVVLLHGWPQTWWEWR
                     HVIPALAKTYSVIAPDLRGLGDTSRPLGGYDKKTIAHDVWLLLAERLGHRSFFLVGHD
                     WGGPTAFALAAAHPEAVRRLVILDVVIPGTGGDFSEGGRRWHHQFHMTADLPEALTQG
                     HERTYLSWFYDTFAYRPDAIGPADLDEYARTYSQPGAMRAGFAYYRALLQDIADNKAL
                     LAQAKLAMPVLAIGGAVSYPHGRGRGSAPEQSLRQAAHDVRGKVIADCGHFIPEEAPE
                     ELIKHLLEFFGEGH"
     gene            1795..2409
                     /locus_tag="VFR00_06180"
     CDS             1795..2409
                     /locus_tag="VFR00_06180"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEU0158877.1"
                     /translation="MPTDGLRRQVREELPEIGLIGDQDLQAKVVEAWAYAIANSSFTS
                     IRDIPPAGNPDVNEARRGDQTDHLRGVTRLAVGIAKEMGSAYPELEIDMDVIIAGGLV
                     HDVGKAWEFDPVNRARWRQAQKQFGRPSLRHPAYGAHICLTVGLPEEIAHIAMAHSGE
                     GELLLRSLECMIVHQADYTFWNTLLAGGALKPETVAPEKRRYVS"
     gene            2502..3350
                     /locus_tag="VFR00_06185"
     CDS             2502..3350
                     /locus_tag="VFR00_06185"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU0158878.1"
                     /translation="MRGKQVLITGATNGIGLAAAKSLAALGANIAIVGRNAVRTRVAV
                     AEIQAQAHRDARVTSFVADLALQAAVRHLAADVLACHARIDVLINNAGAMNRFRQLTQ
                     DGIELTWALNHLAPFLLSTLLLERLIATAPSRIITTASDAHRGAEIPFDDLNAERGSH
                     GFLRYKQTKLANILFAQELAARLAGTGVVSSSFHPGLVASGFNRNNGALMSLAMLALR
                     PLSRSVEQGAETLVWLAQANAQDIDGGYFVDLARRQPSRQARDREAAQRLWQVSERQC
                     AGLAPA"
BASE COUNT          732 a         1389 c         1473 g          799 t
ORIGIN      
        1 gcgccctctt tctgtcggaa atgctgaccg cgagtgccat gcaggcctat cagggcgaga
       61 tcgaaagagc cgtcgtcctc accctcttca ttccgctcat tatgagctcg gggggcaatt
      121 cgggctcgca ggcaacctcg ctcgtaatcc gggcactggc gctgcgcgaa atcggccttg
      181 cggattggtg gcgcattgcc tcgcgcgagc tcccggctgg gctcacgttg ggaaccattc
      241 tgggcgtcct tggcgtggcc cgcatcgtgc tctggcaaca cctggggctt tacgactacg
      301 gcgagcattg ggggctggtt gcgctgaccg tcgggctggc gctgaccgcc atcgtcacgt
      361 tcggctccct aaccggttcc atgctgccgt tcgccttgaa acggctgggc ttcgatccgg
      421 cgacggcatc ggcgcccttt gtggccaccc tcgtcgatgt cagcgggctc gtgatctatt
      481 tcagcgtcgc ctacatggta ttgcgcggca cgctgttgtg aggccgagtg gagttggctg
      541 gcacgggcgg tgccgcggtc ccacccggac gcaggcagac gtgataggct cggcgcttgc
      601 aggaggaagc acgccggcat gaccaccgcg ctcgacgtca gccatcattt cgccgatctg
      661 ggcgaggttt tgatgcatta cgtgacggcc ggcgaggggc cgcccgtcgt cctgttgcac
      721 ggctggccgc aaacctggtg ggagtggcgc catgtgatac ccgccctcgc aaagacctat
      781 agcgtgatcg ccccggatct gcgggggctc ggggatacct cgcgcccgct cggcggttac
      841 gacaaaaaaa ccatcgccca tgatgtctgg ttgttgcttg ccgagcggct ggggcatcgc
      901 tccttcttcc tggtcggaca tgattggggc ggcccgaccg ccttcgcgct ggctgctgcc
      961 catcccgagg cggtacgccg cctcgtcatt ctcgacgtgg tcattcccgg cactggaggg
     1021 gatttttccg aagggggtcg gcgctggcat caccagttcc acatgacggc cgatctgccg
     1081 gaagcgctga cgcagggcca cgagcgcacc tatctttcct ggttttacga caccttcgcc
     1141 tatcggcccg acgccatcgg gcccgccgat ctcgacgagt acgcgcgcac ctatagccag
     1201 ccgggcgcca tgcgtgccgg tttcgcttac tatcgtgcgc tcttgcaaga cattgccgac
     1261 aacaaggcat tgctcgccca ggccaagctg gccatgcccg tgctggcgat cggcggcgcc
     1321 gtgagttatc cgcacgggcg cgggcgcggc agcgcacccg agcaatcgct acggcaggcg
     1381 gcccacgacg tgcgcggcaa ggtgatcgcc gactgcggcc atttcatccc cgaagaggcg
     1441 cccgaggagc tcatcaagca tctgctcgag tttttcggcg aaggccacta aggcgaggcg
     1501 gagctaatta cccccgcgaa ggggtggcgc aaggcagcgc cagcaagctc atcgtcttcg
     1561 ccgggaacgg cgtcgcctct gacatctcgc gagggaggct cctcgcgcgc aagcgcgcgg
     1621 cttcgccttg gcaaaaatgg cgacgatctg gccgggcctg ccctccaggc caagttgcgc
     1681 ggccttgctg ctgctctata gtcccgcaag gtcggcgcag cgattgcggt gacgccgggg
     1741 aaagaggcgg ctgtcatggt cggaaaatca gagagcagat ccagtccggc gcggatgccg
     1801 accgacggcc tgcgccgcca ggtccgggaa gagctgcccg aaattggcct tatcggggat
     1861 caggacctgc aagctaaagt cgtggaggcg tgggcttatg ccattgccaa ctccagcttc
     1921 acgtcgatcc gcgacatccc gccggccggc aaccccgatg tcaacgaggc tcggcgtggt
     1981 gaccagacgg accatttgcg gggtgtgacg cgactggcgg tcggcatcgc caaggaaatg
     2041 ggatcggcct atcccgagct cgaaatcgac atggacgtga ttatcgccgg cgggctcgtg
     2101 cacgatgtgg ggaaagcctg ggaattcgac cccgtcaacc gggcgcgttg gagacaagcg
     2161 cagaaacagt ttggccgtcc atcgctgcgc caccccgcgt atggcgccca catttgcttg
     2221 accgtcggcc tgcccgaaga gattgcgcat attgccatgg cacattccgg cgagggcgag
     2281 cttttgctca gaagtctcga atgcatgatt gtgcatcagg ccgattacac gttctggaac
     2341 acgctgctgg cgggaggcgc gttgaaaccg gaaaccgtcg cgccggaaaa gcggcgctac
     2401 gtcagctgag tctgagacct gcagcgcgcg cctgcaccgg ccgttccccg tctgcgacgg
     2461 cgtgcgctcg gcaattccga tcgaggcccg gaaaggacta gatgcgcgga aaacaggtgc
     2521 tgattacggg cgcaaccaat ggcatcggtc tcgcggccgc caagtccctg gcggcgctcg
     2581 gcgcaaatat cgccatcgtc gggcgcaacg cggtgcgcac gagagtggcc gtcgccgaga
     2641 tccaagccca ggcgcaccgg gatgcgcgag tgacaagctt tgttgcagac cttgcgctgc
     2701 aggcggcggt tcgccacctt gccgcggacg tcttggcgtg tcacgcgagg atcgacgtgc
     2761 tcatcaacaa tgcgggagcc atgaaccgct tccggcagct cacccaggat gggatcgagc
     2821 tcacttgggc gctcaaccat ttggcgccct ttctgctcag cacgctgctg ctcgaacggc
     2881 tcatcgccac ggcaccatct cgcatcatca cgaccgcgtc ggatgcgcac cggggcgccg
     2941 agattccctt cgacgacctc aatgccgagc gcggctccca tggttttctc aggtacaagc
     3001 agacgaagct cgccaacatc ttgttcgccc aggagctggc tgcgcgcctg gcggggacgg
     3061 gggtggtttc gagttccttt cacccggggc tcgtggccag cggcttcaac cgaaacaacg
     3121 gtgccttgat gagcttggcg atgctggcgc tgcgacccct ctcgcgctct gtcgagcaag
     3181 gggcagaaac gctcgtttgg ctggcgcagg ccaacgctca ggacatcgac gggggctact
     3241 tcgtcgatct ggcacggcgc cagccgagcc ggcaagcacg ggatagagaa gccgcacaac
     3301 ggctctggca ggtgagcgaa cggcaatgtg cggggctcgc gccggcctag attcgagcgc
     3361 ccgcgcgagc cgcatttgca tcatgcgcgg cggaacccct cttctcagtt gggtgacacc
     3421 tggcctgctc gctcccaaac cggagctctt gaggacgggg tcgcgccata ccgtcttgca
     3481 tcaccagcgc gtcggaagct ggaggcaatc ggtcgccggc gcacgagaag gggcgaggaa
     3541 gaggtgcggg tgctttcctc gcccaagtgc gctccggatg cgggaccgga gcacggcgcc
     3601 ttgcgggggt tcatgccacc gctcaggccg ccgatttcag gcaagggaaa tgcaggatca
     3661 tctggttgcg attgtggcgt ggtggcagag atctgtggag aattcgccca atcgcaaatc
     3721 cggttgccgc agcagcgggc cgagcgccaa gcccggggca ggacctcgag aggggacaac
     3781 gcgcgcgtaa gtgctggaag gggagacgcg cgcgcggcga gccgcggctc tcgcgctcac
     3841 gtcgccctcg gggctgcgcc tgcctggctg ttgcaattgg cgatggcctc gaattgaggg
     3901 cagctgatcg gcattggcgc catctggcga gccaagggcc gggggcaccc tgcggcgcct
     3961 aagctctgtc ggcgtcgggg gcgacgcgtt tgcccactga ggcgggcagc gccctacgcc
     4021 gcggcaccta tggcggacaa gtccttgcga gttcgccgga ctttggtcag tggcaagccc
     4081 ggtcgagccc atgacaaatg gcaccgttgc ggtgcgggag tattgtgtag cggggcgtgg
     4141 tgctggcgtt tttttgacta tggacgcgtt taaaatcacc tgaaggtctg agtttggaac
     4201 cactcgcctc gcggatccca atccgcgggg tttttccttt tgacgtgctg gcccgaggga
     4261 tcggctccgc ttgctcggcc ctgcgcttgg cctgcaatgg caagctcgcc gcgtcgtgag
     4321 agcatcccga caccggccct gcggacacgg cagtggcggg ggcggtcgag cgtcaatctt
     4381 tgttccgccc ttg
//
LOCUS       DASTMT010000266         4538 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_2111344, whole genome shotgun sequence.
ACCESSION   DASTMT010000266 DASTMT010000000
VERSION     DASTMT010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4538
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2111344"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..181)
                     /locus_tag="VFR00_06190"
     CDS             complement(<1..181)
                     /locus_tag="VFR00_06190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0158879.1"
                     /translation="MAKEAVEPGRSFLLALVFFTFAMNLLARGTQESFAVFLLPVQKG
                     LGLTRPQITLTYAANM"
     gene            complement(202..852)
                     /locus_tag="VFR00_06195"
     CDS             complement(202..852)
                     /locus_tag="VFR00_06195"
                     /inference="COORDINATES: protein motif:HMM:NF015682.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0158880.1"
                     /translation="MRVVDDIVAQFRELATPTIANALDDVEFAGVMGGLAQIVPGTRC
                     VGRAVTIRQVTGARGDFRSEDFKVGDMIEAAAPGDILVIDNAGHCVSTFGGLATLAAK
                     MQAIAGVIVDGGVRDREEMLEQRLPVFARHLTPLTGRTRIAISAINQPVSCGGVRVRA
                     GDLIVADGSGVICVPAEHAAEVARLAGNYARDDAAAAEELARGLSFRQAMAKFTRI"
     gene            1219..1749
                     /locus_tag="VFR00_06200"
     CDS             1219..1749
                     /locus_tag="VFR00_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013949349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0158881.1"
                     /translation="MWRPECGRAYRAAARRLLATALLLLAGGIAEAGPSPEEYIAHVG
                     GDAKIVAFGELALDGRKLVCGKRPTVLDTQLDDYGAAYPGFLILNPKLLSKVSTPVKL
                     WIHAHECGHQFRGPDEETADCFAVQRGRRQGWLTPDGLEEVCRFIAPAKGDSMHFSGS
                     HRCELMRKCYTDSAIK"
     gene            1974..2579
                     /gene="leuD"
                     /locus_tag="VFR00_06205"
     CDS             1974..2579
                     /gene="leuD"
                     /locus_tag="VFR00_06205"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019199127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEU0158882.1"
                     /translation="MEKFTTLTGVAAPMPMINIDTDAIIPKQFLKTIKRTGLGKHLFH
                     ELRYDEEGKERADFVLNKPAYRQAKVLVAGENFGCGSSREHAPWALLDFGIRCVISTS
                     FADIFHNNCFQNGILPIKVSPADLDKLMDDANRGANATITVDLEKQEIRGPDGGVVVF
                     DIDPFRKRCLLEGLDNIGLTLREEKAIDTFEAKAHVERPWL"
     gene            2883..4061
                     /locus_tag="VFR00_06210"
     CDS             2883..4061
                     /locus_tag="VFR00_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HEU0158883.1"
                     /translation="MEGASKEAGEVLPDVVGDSACGHDGLAVAMPTANYVLRSREPPT
                     QRQRYRRAYAALDLGTNNCRLLVAHPRPRGFKVVGAFSRIIRLGEGVSISGRLSEPAI
                     ERTIDALHACANKMRALNVERSGLIATEACRVAINAPEFLRRVQDETGLDIKIVSREA
                     EARLAVSGCASLIDPACDLVLVFDIGGGSSELIWLDLSRHRGFDRSAPADRLELQTAI
                     AAWTSLPVGVVSLAERFGGVFVCPQVFQAMVDHVGTLLAPFEAVHKFRARLPGSRTHL
                     LGTSGTVTTIAGVHLGLPRYDRNRVDGCWLETEQARAVSGRLLAASYEERVAEPCIGK
                     ERADLVLAGCAILEAMLKMWPCDRLRVADRGLREGILATLMAEDGVHRGEHRRGYWRR
                     "
     gene            complement(4046..>4538)
                     /locus_tag="VFR00_06215"
     CDS             complement(4046..>4538)
                     /locus_tag="VFR00_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009029904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SAF domain-containing protein"
                     /protein_id="HEU0158884.1"
                     /translation="GTFVVIASQSPYAKQCFREYHLLPDRSGNYAALYRPTHMIGLEL
                     GVSVASVALRKEPTGAPVGFYSDVVATAKRALKKGEMLDGEGGFCVWGKQTPAEVSLR
                     RGYLPLGLANDVKLTRDIAAGEPLAWTDVVLDATGLAVKVRREMEALFAPDARPQADQ
                     RRQ"
BASE COUNT          762 a         1534 c         1485 g          757 t
ORIGIN      
        1 acatgttggc agcataggtg agggtaattt gcgggcgcgt caggcccaat cccttctgca
       61 ccggcagcag aaagacggcg aatgattctt gcgtgcctcg cgccagcagg ttcatggcaa
      121 aggtgaaaaa taccagcgcg aggagaaaac tcctgcccgg ctcgacggcc tcctttgcca
      181 cgggcgcggc ctcgttggcg cttaaatgcg cgtgaacttc gccatggcct ggcgaaagct
      241 gagcccacgt gcgagctcct ctgcggccgc cgcatcatcg cgcgcgtagt tgccggccag
      301 gcgcgccacc tccgcggcgt gctcggcagg aacgcagatc accccggagc cgtcggcgac
      361 gatcagatcg cccgcccgca cgcgcacgcc gccgcagctg accggttggt tgatggcgct
      421 gatggcaatg cgcgtacgcc ccgtcaacgg ggtcaggtgg cgggcgaaca ccggcagtct
      481 ttgctccagc atctcttcgc gatcgcgcac gcccccatca acgatgacgc cggcaatggc
      541 ttgcatcttg gcggcgagcg ttgccaggcc gccgaaggtc gagacgcaat ggccggcatt
      601 gtcgatgacg agaatgtcgc cgggcgcggc cgcctcgatc atgtcgccga ccttgaaatc
      661 ctcggagcgg aaatcgccgc gcgcgccggt gacctgccgg atggtgaccg cacggccgac
      721 gcagcgtgtg cccggcacga tctgggcgag cccgcccatc acgccggcaa actccacatc
      781 gtcgagggca ttggcgatcg tgggggttgc gagctctcga aattgcgcca cgatgtcatc
      841 gaccacgcgc atgccggtgg ctcctttggc tcaggcgttg gcttgcggga ccctctccta
      901 tcgcccccgc gcgcgcggca caaccgcgcg ccgccatgcc atgcgatggc gctgtgcgct
      961 ggcccggcca acgacggtgg cggggcgcaa ggtggcaggt ttgccacgcc cgcggcgcga
     1021 tcgcgtctga ggaccaacgg cgtcctgaaa ttgcccgtaa gcgcccacat cctgccccgc
     1081 cgcaatcttt gcctgcgtgg gcgacaaggg cggcggcggt agttgcctgc ggagtaggcc
     1141 aaatgatggg ttgcgttgcg gcctgcgagc ggcgaagctg gtcgcgggta ggacctgagg
     1201 ctaagagggg atcacgcctt gtggaggcct gagtgcggcc gagcttatcg cgcagccgcg
     1261 cgtcgcctgc tggcgaccgc cctgctgctc ctcgcgggcg ggatcgccga ggccggccct
     1321 agtcccgaag aatacatcgc ccatgtcggc ggcgacgcca agatcgtggc gttcggcgag
     1381 ctggccctcg atggacgcaa gctggtgtgc ggcaaacgac cgaccgtgct cgacacccag
     1441 ctcgatgact acggggccgc ctatcccggc ttcctcattc tcaatccgaa gttgctctca
     1501 aaggtctcca caccagtcaa gctgtggatc catgcgcatg agtgcggcca tcagttccgc
     1561 ggccccgacg aggagaccgc cgactgcttc gccgtccaac gcggccgccg ccagggctgg
     1621 ctgacgcccg acgggctcga ggaggtgtgc cgcttcattg cgccggccaa gggcgacagc
     1681 atgcattttt ccggttcgca ccgctgcgag ctgatgcgca agtgctatac cgatagcgcc
     1741 atcaaataga tattgccggc gcagggcgct cgcgtcttgg tccggtcgcc agcgatcctc
     1801 cggcgggcga ggcgccgagc gcccgtccgc gcgcgccgag ctgaggccgg cgtcccgccc
     1861 ggttcaccgc tggggccgac cgcgggcacc cttgacgtgg ccaagcgcac tggcgttaaa
     1921 agggtctcga gcggcggtca ttgcggaccg cggcagcagg cttgaggacc acgatggaaa
     1981 agttcaccac cttgacgggc gtcgccgcgc ccatgccgat gatcaacatc gataccgatg
     2041 ccatcattcc caagcagttc ctgaagacca tcaaacgcac cgggctcggc aagcacctct
     2101 ttcacgaatt gcgctacgat gaggagggca aggaacgcgc ggacttcgtg ctcaacaagc
     2161 cggcctatcg ccaggccaag gtgctggtgg ctggagagaa ctttggctgc ggctcctcgc
     2221 gcgagcacgc accctgggcc ttgctcgact tcggcattcg ctgcgtgatc tcgacctcct
     2281 tcgccgacat ctttcacaac aactgctttc aaaacggcat cctgccgatc aaggtctctc
     2341 cggccgatct ggacaagttg atggacgatg ccaatcgcgg cgccaacgcg accatcacgg
     2401 tggatcttga gaagcaggaa attcgcggcc ccgacggcgg cgtcgtcgtc ttcgacatcg
     2461 atccttttcg caagcggtgc ctcctcgagg gcctcgacaa catcggcctg accttgcggg
     2521 aggaaaaagc catcgacacc ttcgaggcaa aggcgcacgt cgagcggccc tggctctagg
     2581 cggtccggcg aacgccggca cgttcaacgt cagagggacc gcgcgcaagg gcgcgcgtct
     2641 accccggcac gaggagttgc cgccggcgcc gcgcccgcgc ctcgcgcggt gacacagcgc
     2701 caacgttccg ccaacgttct aacccctcga ggctgcagcg gcgcccgagc cggccggcgc
     2761 ctgcgtgctt aattccttgc cgcacggcga ccggctttac ccgggcgggg gacctgaata
     2821 gagtaacctc aagttcaagc acaaggacgt ccgagcggga tgaacgggcg ccggagacga
     2881 ccgtggaggg tgccagcaaa gaggccggcg aggttttgcc ggatgtggtg ggcgacagtg
     2941 cgtgtgggca cgatggcctt gccgtggcaa tgccgacagc caactacgtg ctgagaagcc
     3001 gcgagccccc cacgcagcga cagcgctatc gacgcgccta tgccgcgctc gatctcggca
     3061 ccaacaactg tcgtctgctc gtggcgcacc cgcgaccgcg tggcttcaaa gtggtcgggg
     3121 ccttttcgcg catcatccgc ctgggcgagg gcgtctccat cagcggacgt ttgtccgaac
     3181 ccgccattga acgcacgatc gatgcgctgc atgcctgcgc caacaagatg cgcgcgctca
     3241 atgtcgagcg ctcggggctc atcgccacgg aggcctgtcg cgtcgccatc aacgcgcctg
     3301 agttcctgcg ccgcgtgcag gatgagaccg gtctcgatat caaaatcgtc agccgggagg
     3361 ccgaggcacg gctggccgtg tcgggctgtg cctctttgat cgacccggcc tgcgatcttg
     3421 tcctcgtctt cgacatcggc ggcggctcct ccgagctcat ctggctcgac ttgtcgcggc
     3481 accgcggctt tgaccgcagc gccccggccg accggctcga gctgcagact gcaatcgcag
     3541 cctggacctc tctgcccgtc ggcgtcgtca gcctggcgga gcgcttcggc ggggtgtttg
     3601 tgtgtccgca ggtgtttcag gcgatggtcg atcacgtcgg caccttgttg gcccccttcg
     3661 aggcggtcca taaattccgc gcgcgtctgc ccggcagccg cacacacctg cttggaacat
     3721 cggggacggt gacgaccatc gcgggcgtgc atcttggcct gccgcgctat gatcgcaatc
     3781 gcgtcgacgg ctgctggctc gagacggagc aagcccgcgc cgtctcgggg cgcctgctgg
     3841 cggccagcta cgaagagcgg gtggccgaac cctgcatcgg caaggagcgg gccgatctcg
     3901 tgctggcggg ctgcgccatt ctcgaggcca tgctcaagat gtggccctgc gatcgcctac
     3961 gcgtggccga ccgggggctg cgcgaaggca tcctcgcaac cctgatggcc gaggacgggg
     4021 tgcatcgggg cgagcaccgc cgcggctact ggcggcgctg atcggcctgc ggccttgcgt
     4081 ccggagcaaa cagcgcttcc atctcgcggc gcaccttgac ggccaagccc gtggcatcca
     4141 ggaccacgtc cgtccaggcg agcggctcgc cagcggcgat atcgcgcgtg agcttcacat
     4201 cgttggcgag ccccaagggc agatagcccc gccgcagcga aacctcggcc ggcgtctgct
     4261 ttccccagac gcaaaagccg ccttcgccgt cgagcatctc tccctttttc agcgcgcgtt
     4321 tggcggtggc gaccacatcg gaataaaagc ccaccggcgc gcccgtcggc tccttgcgta
     4381 gcgcaacgga ggcgaccgac accccgagtt ccaagccgat catgtgcgtt ggccgataga
     4441 gcgccgcata gttgccgctg cgatcgggca gcaaatggta ttcccgaaaa cattgcttgg
     4501 cgtagggact ttgactcgca atcacgacga acgttccc
//
LOCUS       DASTMT010000267         1579 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_2764234, whole genome shotgun sequence.
ACCESSION   DASTMT010000267 DASTMT010000000
VERSION     DASTMT010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 1579)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1579)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1579
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2764234"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..730
                     /locus_tag="VFR00_06220"
     CDS             <1..730
                     /locus_tag="VFR00_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018183628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HEU0158885.1"
                     /translation="AWPGLEGMDLVPEVTAGQSYTWDETRWAWGKGYGRQQTPELHVV
                     AVDYGLKRNILRCLATAGFDVTVVPATASAAEILERKPDGVFLSNGPGDPAATGTYAV
                     PQIKKLVASGLPIFGICLGHQMLGIAIGAKTRKMHQGHHGANHPVKDYTTGKVEITSM
                     NHGFAVDRDSLPARAEETHVSLFDNSNCGLRLKDAPVFSVQYHPEASPGPEDSHYLFV
                     RFGNLMRERKGLPLLPERPGGVRP"
     gene            866..>1579
                     /locus_tag="VFR00_06225"
     CDS             866..>1579
                     /locus_tag="VFR00_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012317574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase large subunit"
                     /protein_id="HEU0158886.1"
                     /translation="MPKRTDIKSILIIGAGPIVIGQACEFDYSGTQACRALKAEGYRI
                     ILVNSNPATIMTDPELADATYIEPITPEIVAAIIERERPDALLPTMGGQTALNTALSL
                     NSQGVLKRFGVEMIGATAEAIDKAEDRQLFREAMTRIGLATPKSRLANASELKRADRA
                     AHAAELARLEALSEPAEDKERQRADYLKHWKAGAHERQKRYQEQAVLKAMEALSSIGL
                     PAIIRPSFTLGGTGGGIAYN"
BASE COUNT          297 a          557 c          501 g          224 t
ORIGIN      
        1 ggcgtggccg ggcctggagg gcatggacct ggtgcccgag gtgacggcgg ggcaaagcta
       61 tacctgggat gagacgcgct gggcctgggg caagggctac ggccgccagc agacgccgga
      121 gctgcatgtg gtcgcggtcg actacggcct gaagcgcaac atcctgcgct gcctcgccac
      181 cgccggcttc gatgtgaccg tggtgccggc gaccgccagc gccgccgaaa tcctcgagcg
      241 caagcccgac ggggtattcc tctccaacgg ccccggcgac ccggccgcga ccgggaccta
      301 tgcggtgccg cagatcaaga agcttgtggc gtccgggctg ccgatcttcg gcatctgcct
      361 cggccaccaa atgctgggga tcgccatcgg cgccaagacg cgcaagatgc atcagggcca
      421 ccatggcgcc aatcacccgg tgaaggacta caccaccggc aaggtcgaga tcacctcgat
      481 gaaccacggc tttgccgttg atcgggacag ccttcccgcc cgtgccgagg agacgcacgt
      541 ttccctgttc gacaactcaa actgcggcct gcggctcaaa gacgcccccg tgttttccgt
      601 gcagtaccac cccgaggcgt cgccgggacc ggaggacagc cactatttgt tcgtgcgctt
      661 cggcaacctg atgcgcgaac gcaagggcct gccgctgctg ccggaacgcc cgggcggggt
      721 tcggccctag ggccaagcct tggccggtgc ggcgccgctg caacccgcca caaaccccct
      781 gcaaacccct ggcgccgcag gcaataggca gcgctgggca gccgcctata gccaagcccg
      841 cgacgctgca atatgaagcg ggcccatgcc aaaacgcacc gacatcaaat cgatcctgat
      901 catcggcgcc gggccgattg tcatcggcca ggcctgcgag ttcgactatt cgggaaccca
      961 ggcttgccgg gcgctgaagg ccgagggcta ccgcatcatc ctggtcaatt ccaatcccgc
     1021 caccatcatg acggatccgg agctggccga tgcgacctat atcgagccga tcacgcccga
     1081 gatcgtggcc gccatcatcg agcgcgagcg cccggacgct ttgcttccga ccatgggtgg
     1141 gcaaaccgca ctcaatacgg cgctgagcct caacagccag ggcgtcctca agcgcttcgg
     1201 cgtcgaaatg atcggcgcca cggccgaagc catcgacaag gccgaggatc gccagctgtt
     1261 ccgcgaggcg atgacgcgca tcggccttgc cacgcccaag tcgaggctgg ccaacgccag
     1321 cgaactgaag cgcgccgacc gcgccgccca tgccgccgag ctggcgcggc tcgaggccct
     1381 gtcggagccg gccgaagaca aggagcgcca gcgcgccgat tatctaaagc actggaaagc
     1441 cggcgcgcac gagcgccaga agcgctacca ggagcaggcg gtgctcaagg ccatggaagc
     1501 cctttccagc attggtctgc ccgccatcat caggccgtcc ttcaccctcg gcggcaccgg
     1561 tggcggcatc gcctacaac
//
LOCUS       DASTMT010000268         1710 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_4668173, whole genome shotgun sequence.
ACCESSION   DASTMT010000268 DASTMT010000000
VERSION     DASTMT010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 1710)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1710)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1710
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4668173"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(85..1080)
                     /locus_tag="VFR00_06230"
     CDS             complement(85..1080)
                     /locus_tag="VFR00_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013500862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HEU0158887.1"
                     /translation="MAPAETMTAIEISAAGGPDVLKPRVLATPKPGAGQMLVKVAAAG
                     VNRPDVAQRSGSYPPPADASPLPGLEIAGAVAALGDGVTRWRAGDQVCALVNGGGYAE
                     YCLVEDVQALPIPAGLDVVRAAGIPETFFTVWANVFDRGRLAAGEWLLVHGGSSGIGT
                     TAIQLAKAFGAKVIATAGSAAKCRACLDLGADRAVNYRSEDFVTACQEATGGKGVDVI
                     LDMVAGSYTDRNIVAAAPEGRIVQIAVLGGATVSFNISRLMMKRLTLTGSTLRPRSRA
                     FKGFLATSLEAKVWPLLAAGKVQVVLDSTFPLLQAADAHRRMETGEHVGKIVLTV"
     gene            1204..1392
                     /locus_tag="VFR00_06235"
     CDS             1204..1392
                     /locus_tag="VFR00_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1192 domain-containing protein"
                     /protein_id="HEU0158888.1"
                     /translation="MDWDELKPKPAKGITLGEPLESLSIGELEARIAALEAEIERVKD
                     NVVRKRAHEAAADAVFKR"
BASE COUNT          279 a          566 c          563 g          302 t
ORIGIN      
        1 cttgagcccc gccgtcctgc agcagttgtt ccaatcccgt cccttcgcgc cggtggccgc
       61 actccccgcc ctggcctggt gaggctaaac ggtcagcacg atcttgccga cgtgttctcc
      121 cgtttccatg cgccgatgcg catcggcggc ctgaagcagg ggaaaggtgg aatccagtac
      181 gacctgcacc ttgcctgcgg ccagcagggg ccacaccttg gcctcgaggc tggtcgccag
      241 gaagcccttg aaggcgcgcg atcgcggacg cagggtcgag cccgtcagcg tcaggcgttt
      301 catcatcagg cgcgagatgt tgaagctgac ggtggcgccg ccgaggacgg caatctgcac
      361 gatcctgccc tcgggcgccg ccgcaacgat gttgcgatcc gtgtagctgc ccgcgaccat
      421 gtcgaggatg acgtccacgc ccttgccgcc cgtggcctcc tgacatgccg tgacgaaatc
      481 ttcgctgcga tagttgacgg cccggtcggc gccgagatcg aggcaggctc ggcatttcgc
      541 ggccgaaccc gccgtcgcta tcaccttggc tccgaaagcc ttggcgagct gaatggcggt
      601 ggtgccgatc ccactcgagc cgccatgcac cagcagccat tcgccggccg cgaggcgccc
      661 gcggtcgaag acgttggccc agaccgtgaa gaaggtttcg gggatgcctg ccgccctaac
      721 gacgtcgagc ccggccggaa tcggtagggc ctggacgtcc tcaaccaggc agtactcggc
      781 gtagccgcca ccattgacga gggcacagac ctggtcaccg gctcgccagc gcgtgacccc
      841 gtcgcccagc gcggcgacgg cgccggcgat ctcgagcccc ggcagcggcg aggcatcggc
      901 cggcggcgga taagaaccgg aacgctgcgc gacgtcgggg cggttgacgc cggcagcggc
      961 caccttgacc agcatttgcc cggcacctgg cttgggcgtt gccaataccc tgggcttgag
     1021 cacgtcgggg ccgcctgcag cgctgatctc gattgccgtc atggtttctg caggggccat
     1081 ctttcagctc cacggaaggt tcggccgcat cgccaccgcc acagtgaccc ggcgcgcggg
     1141 gttgagacaa gagcgaactc atgtcaggtt gcagggcccc ttgtggtcgt tcgggaggcc
     1201 ggcatggatt gggatgagct caagccgaaa ccggcaaagg gcatcacgct tggcgaaccg
     1261 ctggaaagcc tatcgatcgg cgaactggag gcgcgcatcg ccgcgcttga agcagaaatt
     1321 gagagggtca aggacaacgt tgtgcgcaag agggcgcacg aggctgcagc cgacgccgtg
     1381 ttcaagcgct gaggtcttga gcctcgcggt ggcgcatgcc ctttggttgc cgccacactg
     1441 gtagctacaa gcctgtgccc tgccggcaca cccagcgtgc gccgtagccg ccgacgtgcc
     1501 gattgggcgc gtcgctttac cttttgttat ccaatcagtt gcagtatcgg gctatccagt
     1561 tctctggatc cgagtggatc gcatccactc cgtttgacgc ctccctgtta actcctgagc
     1621 cgccctaggg cggctcattt ttcttgtgcg ctcgagggtg cccttgaaag ggccccatgc
     1681 gcggcggcac agctcgggct tgcggctcgt
//
LOCUS       DASTMT010000269         5385 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_143215, whole genome shotgun sequence.
ACCESSION   DASTMT010000269 DASTMT010000000
VERSION     DASTMT010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 5385)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5385)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5385
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_143215"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(161..1960)
                     /locus_tag="VFR00_06240"
     CDS             complement(161..1960)
                     /locus_tag="VFR00_06240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0158889.1"
                     /translation="MAEPAPPSLTGKVIDYILGRNTLIGLASFMLLLISGYATWHGMR
                     DFIMGVAATETSQGRLLPGGMSLSNDVLVIAVVVALTFLMWLMLRETFGARRQLRERL
                     ITFPLYLFLATWSIGFGYGFWWSLISGEEATRTGLAGLREDARDAAQVVAARLDAVRG
                     QLDSVVGWSDSQMQREETSGGSCGVASAAGRGPLYNARRSVRDQITTLRDGMTKSWLE
                     PVQGDIEQLRQAATALEGGTVEERQRAFEARASDIRGRARNIAARSNQLGRATAAEMR
                     ALASVVAVTPGATGFACYDPTLAERLRQAAAQAEQPAELKLREAVFNEGPAGVANAVK
                     NLWRNIGVYASSLMAYVVSGGQQAAGRTDTGEPITGRDLIALLATIGIDLGLLALAIV
                     NPPREPPSIRPSGTLRRQINEAIDTAIARAGVDREWVRRHFVHHNKASYLVIPNLYSC
                     DPNNKEEAAKALAVNQLAGVLSDLELVRWPKRGARWLWLRSELEALKREESLGSDTDL
                     TEIRQAWRKKTGGSAGEQGDDLVRDAKPLRNHGLFSKAERALQIAGWSERARNDIEIF
                     RLVDTEGLTPLLMVLNEPQARSEARPEPAAGVA"
     gene            complement(2071..3069)
                     /locus_tag="VFR00_06245"
     CDS             complement(2071..3069)
                     /locus_tag="VFR00_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008877419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU0158890.1"
                     /translation="MAEDLGVALIGAGFFGAWHARAIAAVPGLRLVGVCDENPAAADR
                     LAAAYGARALHDWRQLIADRQSEVVVIATPHHRHAAMAIAATEAGKPTLLEKPMAATT
                     AECTAILEAAERGGAPLMIGHLLHFALPSLIARDRLEAGELGRPITGMSTLVKLWMEP
                     NRRAWHLEPDCAGGMLMTAGIHALDLIVWFMGAPVATVFAAAGALMHAQAADDSAFVL
                     LRFADGRFAQVSSIGYRDGAGSYGMDLVCETGTLRLDFARGVSIGRGGTWTALADSAE
                     PEWMQRALEREWQAMLAAVRTGAPVPVPGAYARHIIACIEAALQSSRERREVPVAP"
     gene            complement(3110..4084)
                     /locus_tag="VFR00_06250"
     CDS             complement(3110..4084)
                     /locus_tag="VFR00_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013167637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HEU0158891.1"
                     /translation="MSKPGLLNTGPMMDLINAGVAKDFAVYGLHEVGDREALFAEVGG
                     AIRALCTGSHTGVRTDAAMLDRLPNLKIIGNFGVGYDTIDIAEAARRGIVITNTPEVL
                     SEEVADTAIGLLLGTIREFYGAEKWLREGRWPRQGDYRLTAASLRDRSIGIMGMGRIG
                     QAIARRLEAFGRPIGYWARHRQPAVSYRYYSDLIAMARDVDTLIAILPGGPATNNLVN
                     APVLEALGPRGVFINVARGSVVDEAALIDALKSRKILRAGLDVFLAEPNINPAFFQLD
                     NVTLVPHIGSASIHTRSRMGQLVVDNLLAFAAGKPPVTPVPETPFRGW"
     gene            4188..>5385
                     /locus_tag="VFR00_06255"
     CDS             4188..>5385
                     /locus_tag="VFR00_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEU0158892.1"
                     /translation="MAPPEYDIAVLGAGHNGLTCAAYLAGASLRVVVLEKNPVVGGAA
                     VTEEFFPGFRNSVAAYTVSLLNPAVIADLELAKFGLKIVERPVANFWPVDAARALLLP
                     YGQANRQAAIAPFSAADAARLPAYEAALERAAGHLRQLALARPPNVGGGWREVLTSAG
                     MARRLYGLTLEDKRLLLDLFSKSAADFLAQWFESEVVKGAFAFDGIVGAYASPYTPGT
                     AYVLLHHCFGEVNGKVGVWGHAIGGMGAISEALAGAARARGALIRTGAGVAALLVEGG
                     AARGVRLETGEIVRARAVAASVAPKLLFRDLIPADALAPDLRRRFLELKSGSGTFRMN
                     VALAELPDFRCRPGKMAADHHASGIIIGPTLAYLDQAYLDARAHGWSLQPVIEMVIPS
                     TLDPSLA"
BASE COUNT          854 a         1814 c         1800 g          917 t
ORIGIN      
        1 cctcctacag ttgccgcgtc tgccgcatgg tgacgacgtc ctgtcgggtt ggaggcggcc
       61 cccggcgcgt ctgccaggaa ttgcgctgca actggggttg atgcggcagc ggtgcggcgg
      121 ttcaccctgg cttccaaagg ggttgcgacc gcgcgagagc tcaagcgacc cccgcggcag
      181 gctccggccg cgcctcgctg cgcgcctgcg gttcgttcag caccatcaag agcggcgtca
      241 agccttcggt gtccaccagg cgaaagatct cgatgtcgtt gcgggcccgc tcgctccagc
      301 ccgcgatctg caaggcccgc tcggctttgg aaaagaggcc atggttgcgc aggggcttgg
      361 catcgcgcac gagatcatca ccctgctcac cggcgctgcc tccggttttt ttgcgccagg
      421 cctgccggat ctcggtgaga tcggtgtcgc tgccgaggct ttcctcgcgt ttgagcgcct
      481 cgagttcgct tctgagccac agccagcgcg cgccgcgttt cggccaacgc accaattcca
      541 ggtcgctcag gacacccgcc agttggttga cggccaaggc tttggcggct tcttccttgt
      601 tgttgggatc gcagctgtaa agattgggaa tcaccagata ggacgccttg ttgtggtgca
      661 cgaaatgccg gcgcacccac tcacgatcga cgccggcgcg ggcaatggcg gtatcgatgg
      721 cctcgttgat ttgccgccgc agcgtgccgg agggccggat cgagggtggc tcgcgcggcg
      781 ggttgacaat cgcgagcgcc agtagcccca aatcgatgcc gatggtggcc aacagcgcga
      841 tcaagtcgcg acccgtgatg ggttcgcccg tgtcggttcg gccggcggcc tgctggccgc
      901 cgctcaccac gtaggccatc agactgctgg cgtagacgcc aatattgcgc cagaggtttt
      961 tgaccgcatt ggcgaccccg gcggggccct cattgaagac ggcttcgcgc agtttgagct
     1021 ccgcgggctg ctcggcctgc gccgccgcct gccgcaggcg ctccgccagg gtgggatcgt
     1081 agcaggcaaa gccggtggcg cccggcgtca cggcgacgac gctcgcaagc gcgcgcatct
     1141 ccgccgccgt cgccctaccc agttgattgc tgcgtgcggc gatgttgcgc gcccggccgc
     1201 ggatgtcgga ggccctggcc tcgaaggccc gctgccgttc ctccacggtg ccgccctcga
     1261 gcgccgtggc ggcctggcgc agttgctcga tatcgccctg caccggctcc aaccaggact
     1321 tggtcatgcc gtcgcgtagg gtcgtgatct ggtcgcgtac gctgcgccgc gcgttataga
     1381 gcgggccgcg tcccgccgcc gatgccaccc cgcaactgcc accgctcgtt tcttcgcgct
     1441 gcatttggct gtccgaccag cccaccacgc tgtcgagctg gccgcgcacg gcgtcgaggc
     1501 gtgccgccac cacttgcgcg gcatcgcgtg catcctcacg caaccccgcc aggccggtgc
     1561 gcgtcgcctc ctcgccggag atcaggctcc accaaaagcc ataaccaaaa ccgatcgacc
     1621 aagtggcgag aaacagatag agcggaaagg tgatcagccg ctcgcgcagt tggcgccgcg
     1681 caccgaaagt ttcgcgcaac atcagccaca tcaaaaaggt gagcgcgacg accaccgcaa
     1741 tcaccaggac atcgttggag agcgacatgc cccccggcaa caggcgaccc tggctcgttt
     1801 cggttgccgc gacgcccata atgaaatcgc gcatgccgtg ccaggtggca taacccgaga
     1861 tcagcaacag catgaaggag gcgaggccga tcagcgtgtt gcgcccgagg atgtagtcga
     1921 tcaccttgcc tgtcagggac ggcggtgccg gctcggccat ggccaactcc tcaatgttcg
     1981 gcgtgcgcgc tgataacgca aaacggcggc cttgttgcct ttcccggcgg ccacgtctgc
     2041 cgttttcggg caaggtgagg tggcgctacc tcagggcgcc accgggactt cgcgtcgttc
     2101 gcggctcgat tgtaatgccg cctcgatgca cgcgatgatg tggcgagcgt aggcgccagg
     2161 cacgggaacg ggggcgccgg tgcggaccgc agccagcatg gcctgccatt cacgctccag
     2221 tgcccgctgc atccactcgg gctccgccga atcggcaagc gccgtccaag tgccgccgcg
     2281 gccgatcgaa acaccgcgcg caaaatcaag gcgcagcgtc ccggtctcgc agacgaggtc
     2341 cataccatag ctgccagcgc cgtcgcggta gccgatgctg gagacctgtg cgaaacgccc
     2401 atcggcgaag cgcagcagca caaacgcgct gtcgtcggcc gcctgggcgt gcattaaggc
     2461 cccggcggcg gcgaacaccg tcgcgacggg ggcgcccata aaccagacaa tgagatcgag
     2521 ggcgtgaatg ccggccgtca tcagcatgcc gccggcgcag tccggttcga gatgccaggc
     2581 gcgccggttc ggctccatcc aaagcttgac gagcgtgctc atgcccgtga tcggccggcc
     2641 cagttcgccc gcctccaggc ggtcgcgggc gatcaggctc ggcagggcaa aatgcaggag
     2701 gtggccgatc atcagggggg cgccgccgcg ctcggccgct tccaggatcg ccgtacattc
     2761 ggccgtcgtc gcggccatgg gcttctccag cagcgtgggc ttacccgcct cggtggcggc
     2821 gatcgccatg gccgcgtgcc ggtggtgcgg cgtcgcaatc accaccacct cgctctgcct
     2881 gtcggcaata agctggcgcc aatcgtgcag cgcgcgggcg ccgtaggcgg cggccaggcg
     2941 gtcggccgcg gccgggttct catcgcatac gcccaccagc ctgagacccg gcacggcggc
     3001 gatggcgcgc gcatgccatg cgccgaaaaa gccggcgccg atcagtgcga cccccaggtc
     3061 ctcggccata gacgcacctc cctcgacagg cgagccgcaa cgcagcgcgt caccaccccc
     3121 ggaacggcgt ctccggcacc ggggtcaccg gcggcttgcc cgcggcaaag gccaacagat
     3181 tgtcgaccac gagttggccc atgcggctgc gggtgtggat cgaggccgag ccgatgtgag
     3241 gaaccaaggt gacgttgtcc aattggaaga acgccggatt gatgttgggt tcggcaagaa
     3301 aaacgtcgag gccggcgcgc aggatcttgc gcgacttcag cgcgtcaata agagccgcct
     3361 cgtccaccac cgagccgcgg gcgacgttga tgaagacgcc gcgtggcccc agggcctcga
     3421 gtacgggcgc gttgacgaga ttgttggtcg ccggcccacc cggcagaatg gcaatgaggg
     3481 tatcgacgtc gcgggccatg gcaatgaggt cgctatagta gcgataactg acggcgggct
     3541 gccgatggcg ggcccagtag ccgatcggac ggccgaaggc ctcgagacgg cgggcaatgg
     3601 cttggccgat gcggcccatg cccatgatgc cgatcgagcg atcccgcagc gaggccgcgg
     3661 tcaagcggta gtcgccttgt ctaggccaac gcccctcgcg cagccatttt tccgcgccgt
     3721 agaactcgcg gatggtcccg agcaacaggc cgatggcggt gtcggcgacc tcttcgctga
     3781 gcacctccgg cgtgttggtg atgacgatgc cgcgccgtgc cgcctcggca atgtcgatgg
     3841 tgtcgtagcc aacgccgaaa ttgccgatga tcttgaggtt gggcagccgg tcgagcatgg
     3901 ctgcatcggt ccgcaccccc gtgtgagagc cggtgcagag cgcgcgaatg gcgccgccga
     3961 cctcggcgaa tagcgcctcg cggtcgccca cctcgtgcag cccgtagacc gcgaagtcct
     4021 tggctacgcc cgcgttgatg agatccatca ttgggccggt gttcaggagc ccaggcttcg
     4081 acatttgcaa ctccgcgctg acttccttaa gtgggtcgta gcattcaagg gcccgaacgt
     4141 tcaattgagg cgtgtgagtt aacaaggcaa ggtgagcttt accgtcgatg gcgccgcctg
     4201 aatacgacat cgccgtgctc ggggccggcc ataacggtct cacctgtgcc gcctatttgg
     4261 ccggcgccag cttgcgcgtc gtcgtgctgg aaaagaaccc ggttgtgggc ggcgccgccg
     4321 tgaccgagga gttttttccg ggtttccgta attccgtcgc cgcctacacg gtgagcctgc
     4381 tcaatcccgc ggtgatcgcc gaccttgagc ttgccaagtt cggcttaaag atcgtggagc
     4441 ggcccgtcgc caatttctgg ccggtggatg ccgcgcgcgc tttgctgctg ccctatgggc
     4501 aggccaaccg acaggcggcc atcgcgccgt tctctgccgc cgacgcggcc cgcctgcccg
     4561 cgtacgaggc ggccctggaa cgggccgcgg gccacctgcg ccagctcgcg ctcgcgcgtc
     4621 cgcccaatgt gggcggggga tggcgcgaag ttctcaccag cgccggcatg gctcgtcgac
     4681 tttacggctt gacgctcgag gacaagcgcc tgctgctcga cctattcagc aagagcgccg
     4741 ccgatttcct ggcgcaatgg tttgaaagcg aggtggtgaa gggggccttt gctttcgatg
     4801 gcatcgtcgg cgcttacgcc agcccatata cgcccggtac ggcctatgtg ctgttgcacc
     4861 actgtttcgg cgaggtcaac ggcaaggtcg gcgtttgggg ccacgccatc ggcggcatgg
     4921 gggcgatcag cgaggcgctt gcgggcgccg cccgcgctcg tggcgcgctg atccgcacgg
     4981 gggccggcgt cgccgcgctg ctcgttgagg gaggggccgc ccgcggcgtt cggctcgaga
     5041 cgggggagat tgtgcgggcc cgcgccgtgg ccgccagcgt ggcgccgaaa ctcttgttcc
     5101 gcgacttgat ccccgccgat gcgctggcgc ccgatctgcg ccgccgcttt ctcgagctca
     5161 agtccggttc gggcacgttc cgcatgaatg tggccctcgc cgagctgccg gatttccgct
     5221 gccgcccggg caaaatggcc gccgatcacc atgcctcggg catcatcatt ggaccaaccc
     5281 ttgcctacct cgatcaggcc tatctcgatg cccgcgcgca tggctggtcg ctgcagccgg
     5341 tgatcgagat ggtaattccc tcaaccctgg atccctccct ggcgc
//
LOCUS       DASTMT010000270         2155 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_143814, whole genome shotgun sequence.
ACCESSION   DASTMT010000270 DASTMT010000000
VERSION     DASTMT010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2155)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2155)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2155
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_143814"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1014
                     /locus_tag="VFR00_06260"
     CDS             <1..1014
                     /locus_tag="VFR00_06260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0158893.1"
                     /translation="DRASARKAAELGVPAILSALTERLGRAGGLARLTQALAMGSSGP
                     KAEGSGGIKFLLGGERAGALASAIGRFVGARESSTLAFLDELSAAILGALGQASGDAN
                     ADGRRVANVLKAERDDIAAAIPSGLVSLLGAPTVQQRPGEPVLPLSALPFGSPDVAPG
                     ARRSALSRAAWAVALIASAGVAWYVLAGVNPPPSLEIGDGVVLGLPGSDVRPAGQAAT
                     PATARLQALHGSTSAGRPASSLTDTFGGSEGPIATSLLRVGRFFGIGRGSVALSVTDL
                     PPWPDRGLAEARRPADAAKAQVSGPSAPVLAPQPLRFVTEEFRDAILPAPEAPAGTRV
                     STK"
     gene            complement(1394..>2155)
                     /locus_tag="VFR00_06265"
     CDS             complement(1394..>2155)
                     /locus_tag="VFR00_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018273241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HEU0158894.1"
                     /translation="AYPGALPHMLYAGSLVFRRPLERVQADHWGHWWNFMKGADWRHP
                     LGPGSTIEGIGGHPVVHVAYQDALAYARWAGKDLPTEAEWEFAARSEEEGAEFAWGDD
                     LHPGGKMMANTWQGQFPWQNLAQDGYERTSPVGAFPANDHGLYDMIGNVWEWTSDWFS
                     ARHQPDAAKPCCIPKNPRGGEENQSYDACQSGSRIPRKVLKGGSYLCAPNYCRRYRPA
                     ARHPQPIDTSTCHVGFRCVARNQPKLSLRSQRSVR"
BASE COUNT          317 a          683 c          762 g          393 t
ORIGIN      
        1 gatcgtgcgt ccgcccggaa ggcggcagag ctcggggtgc cggcaatcct ttccgcgctg
       61 accgaacgct taggtcgggc cggcgggctc gcgcgcttga cgcaggcgct ggccatgggc
      121 tcgagcgggc cgaaggcgga gggttctggc ggcatcaagt ttctgctggg tggcgagcgt
      181 gccggtgcgc ttgcctcggc catcggtcgc tttgtcggtg cgcgtgagag ctcaacgctg
      241 gcctttctgg acgaactgtc ggccgccatc ctgggcgccc ttgggcaggc gagcggggac
      301 gcgaacgccg acggccggcg cgttgccaac gtgctcaagg cagaacgcga cgacatcgcc
      361 gcagcaatac cgtcgggact agtgagccta ttgggcgcgc cgaccgtgca gcagcgtccc
      421 ggcgagccag tgttacccct gtcggccctc ccctttggca gcccagatgt tgcgccaggt
      481 gcgcgccgta gcgcgctcag ccgggccgca tgggccgtgg cgctcatcgc gtcagcgggt
      541 gtggcctggt acgtgctggc tggcgtgaac ccgccgccaa gcctcgagat cggcgacggt
      601 gtggtcttgg ggttgccggg gtcagacgtt cgccctgcgg ggcaggccgc gacgcctgca
      661 acagcccgat tgcaagccct ccatggctcg acgagcgcag ggcgcccggc ttcgagcttg
      721 accgatacgt tcggcggctc cgaggggccg atcgcgacct cgctgctacg tgtcggccgc
      781 ttttttggca ttggccgggg cagcgttgcg ctctcggtga cagatctgcc gccttggccg
      841 gatcggggcc tcgcggaggc gcgccggccc gctgacgcgg ccaaagcgca agtgagcgga
      901 ccttcggcgc ccgtcctggc gcctcaaccc cttcgctttg tgaccgagga gttcagggat
      961 gcgatcctcc ccgcgccgga agctccggcg ggcacgcggg tctcaaccaa ataagcgatg
     1021 ccggcaattg gaaagattcg gctgtccgag gttatgacac aatgactact gcggttggca
     1081 gggcatgcct ggtctttgtt tccatccatg tattgatgat cgtcggcatt ttgctgctgt
     1141 ccggctgaga tccgtgattg gggagggtgg cctgcggcag tagccgcgac ctgacggcca
     1201 gcattgcgct cgcagacggg aggaagggtt atgtcgcccc tcgctcgcaa tcagctcgaa
     1261 ctgcggcgcc gtgcgccccg cggccttgac gtctgacagc gcgcggagct tccgaggact
     1321 gggccggatg gccgggccgc gacgcgttgg gcgatctggg tctccccccc ttccgagtta
     1381 acggctcggg ggcttagcgc acgctgcgct gggaccgcag cgacagtttt ggctggttgc
     1441 gtgcgacgca gcggaagccg acgtgacacg tcgacgtgtc gatcggttgg gggtgccgag
     1501 ccgcgggcct atagcgcctg cagtagtttg gggcacacag ataggaacca cccttgagga
     1561 ccttgcgcgg tatgcggctt cccgactggc aggcgtcgta gctctggttt tcctccccgc
     1621 cgcgcgggtt ctttgggata cagcatggct tggcggcatc gggctgatga cgcgcactga
     1681 accagtccga ggtccactcc cagacgttgc cgatcatgtc ataaaggcca tggtcgttgg
     1741 cagggaacgc accgaccggg gacgtgcgtt cgtagccgtc ctgggccagg ttttgccagg
     1801 gaaactgacc ctgccaggtg ttggccatca tcttgccgcc cggatgcagg tcgtcgcccc
     1861 aagcgaattc ggcgccctcc tcctcgctgc gcgcggcaaa ctcccattcc gcttcggtgg
     1921 gcaaatcctt gcccgcccag cgcgcgtagg cgagcgcatc ttggtaggcg acgtgaacca
     1981 cggggtggcc gccgataccc tcgatggtgc tgccagggcc gagagggtgg cgccagtctg
     2041 cgcctttcat aaaattccac cagtggcccc aatggtcggc ttggacgcgc tcgagcggac
     2101 ggcggaacac gagagatccc gcatagagca tatgtggtag ggcgccggga taagc
//
LOCUS       DASTMT010000271         5061 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_2768006, whole genome shotgun sequence.
ACCESSION   DASTMT010000271 DASTMT010000000
VERSION     DASTMT010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 5061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5061)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5061
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2768006"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..236
                     /locus_tag="VFR00_06270"
     CDS             <1..236
                     /locus_tag="VFR00_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018855599.1"
                     /note="with UgpABC is involved in uptake of
                     glycerol-3-phosphate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HEU0158895.1"
                     /translation="FVILFVYGWNQYLWPLLVATDPRLDTVVIGIVKMIGIETATEWN
                     LVMATAVLALLPPVAVVLLMQRWFVKGLVETEK"
     gene            388..1467
                     /gene="ugpC"
                     /locus_tag="VFR00_06275"
     CDS             388..1467
                     /gene="ugpC"
                     /locus_tag="VFR00_06275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018262061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HEU0158896.1"
                     /translation="MAAVTLNNVRKVYPGGVEAVKGVSLAIPNGSFTVLLGPSGCGKS
                     TLLRMIAGLETVSEGSVSIGDRLVNDVEPADRDIAMVFQNYALYPHMSVYDNMAYGLR
                     NRGVPKADIAVRVAEAARLLAIEDFLKRKPRALSGGQRQRVAMGRAIVREPQAFLFDE
                     PLSNLDAKLRVQMRVEIRRLHNRLKATSIFVTHDQVEAMTLADMVVVMNGGRIEQSGG
                     PTAVYRLPATRFVATFIGSPAMNLLPGRIAGPGIAELKGGRVAFRAEAFACEIGQQVE
                     VGVRPEDLAFTAAGVGEGLAFVRDFVEELGATRLIHGSVGEAPIVVAVATSTALDGAG
                     ALAVDPAAVHLFDIRTGASLRRGLA"
     gene            complement(1485..2384)
                     /locus_tag="VFR00_06280"
     CDS             complement(1485..2384)
                     /locus_tag="VFR00_06280"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU0158897.1"
                     /translation="MRRLCEGRVAIVTGAGGGLGRQHALLLAAHGAKVMVNDIGARVD
                     GTGGDAAAATAVVDSIKQAGGTAIANAADVSDWKGAQTMIAEAVDTFGSLDILINNAG
                     ILRDRMLTNMAEDEWDAVIRVHLKGTFAPSRHAAAYWREQSKNANAPIKARIINTTST
                     SGIYGNVGQTNYGAAKAGIAAFTIIAARELARYGVTVNAISPSAQTRMTEGLRELSEQ
                     ERARRHPKWVSPAVVYLASEEAQDITGRVIQAGVGRLGVCEGWRRGAEVDQVEDPVMA
                     GSLLRAMIAKVRKNVGMDGLELD"
     gene            2614..3354
                     /locus_tag="VFR00_06285"
     CDS             2614..3354
                     /locus_tag="VFR00_06285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0158898.1"
                     /translation="MREDRLGAIAAQLIAARQQGTRISLSAAEWPNDFEEGFAIQERV
                     VTALGSPIIGWKVTPVANGAVIYAPILAAGRVEADATWTVIGPEPAGLELEIAFRLRR
                     SVAAEASAEEVLEAVAAAHVVFELCQSRIADPAALPRQVGLADCIANAGIVVGPEIAG
                     WRDQDLRARPGRLLVDGMVHAAGRSLDPVAALLLLPPAMGRRGRRIEAGQIVITGSLI
                     GMNWLTGRHDLKGIIEGCGEVAMTLVAA"
     gene            complement(3423..4382)
                     /locus_tag="VFR00_06290"
     CDS             complement(3423..4382)
                     /locus_tag="VFR00_06290"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009030021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU0158899.1"
                     /translation="MTASAQRLGWIGMGRMGYPMAERLLKAGHSVAIWNRTIAKAQPL
                     AAAGAKIVGSPVDLAGVDVLFSIVSTGKDLAEVYFGPAGALSVPGKVPAIVVDCSTIG
                     VEESAQIREKLRKQGVDYIAAPVSGNAKVIKAGRLSAVASGREPAFHKVASLIEAFAP
                     AGVSYVGEGELARVCKIAHNVMLGVVIENLIEITLLANKMGVPRHAFLAFMNSSVMGS
                     MFTRYKSPALVNLDWTTTFTPELLRKDLDLGLELGRKMEVPMPVTSAARDMLQSHIGA
                     IRLQPDAANHLAADFAALLETMARASGMRLESEQETVPTGLEG"
     gene            complement(4522..>5061)
                     /locus_tag="VFR00_06295"
     CDS             complement(4522..>5061)
                     /locus_tag="VFR00_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011473752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine reductase"
                     /protein_id="HEU0158900.1"
                     /translation="RFHGLPTNRSHRVTLEVDCPELVTRCQADGVDAALLLPNCPVCH
                     QSAGLAARALESAGIATVVMGCAKDIVEFVGVPRLLFSDFPLGNPAGRPKDPASQRAT
                     LDFALDLLTSASQPRTTVQSPLRWSDSGDWKLDFSNIARLSPEEIARRRAEFDQQKAI
                     ARTVREDAGVTRASANTQA"
BASE COUNT          793 a         1647 c         1726 g          895 t
ORIGIN      
        1 tgttcgtgat cctgttcgtc tatggctgga accagtatct ctggccgctg ctggtggcga
       61 cggacccccg gctcgacacg gtggtgatcg ggatcgtgaa gatgatcggc atcgagactg
      121 cgaccgaatg gaacctggtg atggcgaccg cggtgctcgc gctgctgccg cccgtcgcgg
      181 tcgtgctgct gatgcagcgc tggttcgtca agggcctggt ggagaccgag aagtgagccc
      241 gctgccgcta gaccgcgcct cggcgcgccg ccggcagccg cccggcgcgc gcgctactgg
      301 cattgtgatc gggcgcgggc gtgccgcggg cgcgcgtgcg ctgcgggcag ggcgcgccgc
      361 attggggccg ccgacgggga gccgcacatg gccgcggtga cacttaacaa cgtgcgcaag
      421 gtctatcccg gcggtgtcga ggccgtgaag ggcgtctcgc ttgccatccc gaacggctcg
      481 ttcaccgtgt tattgggccc ctccggttgc ggaaagtcca cgctgctgcg gatgattgcc
      541 ggtcttgaga ccgtgtcgga gggcagcgtc agtatcggcg atcgcctggt gaacgacgtc
      601 gaaccggccg atcgcgacat cgccatggtg ttccagaact acgcgctcta cccgcacatg
      661 agcgtctacg acaacatggc ctacggcctg cgcaaccgcg gcgtgccgaa ggccgacatt
      721 gccgtgcgcg tggcggaagc ggcgcgcttg cttgccattg aagattttct caagcgcaaa
      781 ccgcgtgccc tctcgggtgg ccagcggcag cgcgttgcca tgggtcgggc gatcgtgcgt
      841 gagccgcaag cctttctgtt cgacgagccg ttgtccaacc tcgatgcgaa attgcgcgtg
      901 cagatgcggg tcgaaatccg gcgcctgcac aacaggctca aggcaacctc gattttcgtc
      961 acgcacgatc aggtcgaggc catgacgctg gccgacatgg tggtagtgat gaatggcggc
     1021 cgcatcgagc aaagcggcgg gccgaccgcc gtctaccgcc tgccggcgac gcgcttcgtg
     1081 gccacgttca ttggctcgcc cgccatgaat ttgctgcctg gacgcatcgc tggcccgggt
     1141 atcgccgagc tcaagggtgg acgcgtcgcc ttccgggccg aagccttcgc ctgcgagatc
     1201 ggccaacagg tggaggtcgg cgtccggccc gaggacctgg ccttcaccgc cgccggtgtc
     1261 ggcgaggggc tggccttcgt cagggacttc gtcgaggagt tgggcgccac gcgcctcatt
     1321 catggtagcg tcggggaggc gcccattgtc gttgcggtgg cgacctcgac ggctctcgat
     1381 ggcgccggtg ccttggccgt cgatccggcg gccgtgcacc tgttcgacat ccgcacgggc
     1441 gctagcctgc ggcgtggtct cgcctgagca gccaccggcc tcggtcaatc gagctcgagg
     1501 ccgtccatgc cgacattctt gcgtaccttg gcgatcattg cgcgcagcag cgagccggcc
     1561 atcaccgggt cctcgacctg gtcgacctcg gccccgcgcc gccagccctc gcatacgcca
     1621 aggcggccga cgccggcctg gatcacccgt cctgtgatgt cctgggcttc ctcactggcc
     1681 agatacacca cggcgggtga aacccacttg gggtggcgcc gggcgcgctc ttgctcgctc
     1741 agctcgcgca gtccctccgt catgcgggtc tgcgccgagg gcgaaatggc attgacggtg
     1801 acgccgtagc gggcgagctc gcgcgcggcg atgatggtga aggcggcgat gcccgccttg
     1861 gcggcgccgt agttcgtctg cccgacgttg ccgtaaattc ccgaggtcga cgtggtgttg
     1921 ataatgcggg ccttgatggg agcgttggcg ttcttgctct gctcgcgcca ataggcggcc
     1981 gcgtggcgcg agggggcgaa cgttcccttg aggtggaccc taatcacggc atcccactcg
     2041 tcctcagcca tattcgtcag catgcgatcg cgtagaatgc cggcattgtt gatgaggata
     2101 tccaggctgc cgaaggtgtc gacggcctct gcgatcatgg tttgcgcgcc cttccagtcg
     2161 ctgacatcgg cagcattggc aatggccgtg ccaccggcct gcttgatgct gtccaccacg
     2221 gccgtggccg cggccgcatc accgcccgtg ccatcaacgc gggcaccgat gtcgttgacc
     2281 atgaccttgg cgccatgggc cgcaagcagc agggcgtgtt ggcgtccaag gcctcctccc
     2341 gcgcctgtca cgatcgccac tcgaccttca cataagcggc gcatgtcatc ctcctctcgc
     2401 atgccggtga ccgtcgtgca cagtttggcg gcgggccgat gtgccaggca agccccaaaa
     2461 gctatcgggt taacgcgcgc ggccgggccg acgcggatgc cttgagcgcg cttgccgcaa
     2521 tttcctttcg ggtcgcggcg cagccatgcg atatcccgct ctggcgccgg cggccggcga
     2581 ccaaggttcg atcgcactgg ggagcaaatc gacatgcgcg aggatcgtct cggcgccatc
     2641 gccgcgcaac tgattgcggc ccgccaacag ggcacgcgaa tttcgctctc cgcggcggaa
     2701 tggcccaacg atttcgaaga agggttcgcc atccaggaaa gggtggtgac ggcgctcgga
     2761 tcgccgatca tcggctggaa ggtgacgccc gtggcaaacg gagcggtgat ctatgcaccc
     2821 atcctcgccg cgggtcgagt ggaggccgac gccacctgga cggtgatcgg gcccgagccg
     2881 gccggtctag agctcgagat tgcctttcgc ttgcggcgaa gcgtggccgc cgaggcctcg
     2941 gccgaggaag tcctcgaggc ggtcgcggcg gcccatgtgg tgtttgagct gtgccagagc
     3001 cggatcgccg atccggcggc tttgccgcgc caggtggggc ttgccgactg cattgccaat
     3061 gccggcatcg tggtcggacc cgagatcgct ggctggcgcg accaggatct cagggcccgg
     3121 ccgggcaggc tgctggtcga cggcatggtc cacgcggcgg ggcgcagcct cgacccggtc
     3181 gccgccttgc tgttgctgcc acccgccatg gggcgccgcg gtaggcggat cgaagccggt
     3241 caaatcgtca ttaccggtag cctgatcggc atgaactggc tgacgggacg gcacgatctc
     3301 aagggcataa ttgagggttg cggcgaggtc gccatgaccc tggtcgccgc ctgagccgcg
     3361 gccaccagcc gcggcacgcc acagcgactg ccgtgccgcg cgccccgggg cccgcgtaag
     3421 ccttagcctt caaggcccgt cggcaccgtt tcctgctcgc tctcaagtcg catgccggac
     3481 gcccgcgcca tggtctccag caacgccgcg aaatcggcgg cgagatggtt agcggcatcg
     3541 ggctgcaagc ggatggcgcc gatatggctc tgtagcatat cgcgcgccgc cgaggtcacc
     3601 ggcattggca cctccatctt gcgaccgagc tcaagcccga ggtcgagatc cttgcgcagc
     3661 agttccggcg tgaaggtggt ggtccagtcg agattgacga gcgcgggtga cttgtagcgg
     3721 gtgaacatcg aacccatcac gctgctgttc atgaaggcca aaaaggcatg ccgtggcacg
     3781 cccatcttgt tcgccagcag ggtgatctcg atcaggtttt cgatgacgac ccccagcatg
     3841 acattgtggg cgatcttgca cacccgcgcc aattccccct cgccgacgta ggacacgcct
     3901 gccggagcga aggcttcgat cagtgacgcc accttgtgaa aggcgggctc gcgaccggag
     3961 gcaacggccg acagcctgcc ggctttgatg actttggcat tgccgctcac cggcgcggcg
     4021 atgtagtcga ccccctgctt gcgtaatttc tcgcgaattt gagccgattc ctcgacgccg
     4081 atggtcgagc agtcgaccac gattgccggc accttgccgg ggaccgagag cgcgccggcg
     4141 gggccgaaat agacctcggc caggtccttg cccgtcgata cgatggaaaa cagcacatcg
     4201 acgccggcaa ggtcgaccgg cgagccgacg atcttggccc cggcggctgc cagcggctgg
     4261 gctttggcga tggtgcggtt ccagatggca acgctgtggc cggccttcag caggcgctcc
     4321 gccatcgggt agcccatgcg gcccatgccg atccaaccga ggcgttgcgc gctagctgtc
     4381 attgctgtct ccgtggcatg ccgtggttgg ccgaggccag tttctttgag cgcccgtggc
     4441 gagtgtcatg gtcgtcggcg cgcgcaattg agcccaggcc cggccaacgt gtcaacgaga
     4501 tccccggcgc gggcgtgacg atcaagcctg cgtgttggcg cttgcacgcg tgacgccggc
     4561 gtcctcgcgc acggtgcgcg caatcgcctt ttgctggtcg aactcggcgc gacggcgtgc
     4621 gatctcctcc ggcgacaggc gggcgatatt ggaaaaatca agtttccagt cgccactgtc
     4681 gctccagcgc aaaggtgact gcacggtggt gcgcggctgg ctggccgatg tcagcagatc
     4741 gagggcaaaa tcgagcgtgg cgcgctgcga ggccggatcc ttcggtcggc ctgccggatt
     4801 accgagagga aaatctgaaa acagcaggcg cggtacaccc acgaattcga cgatgtcttt
     4861 ggcgcagccc atcaccaccg tggcaatgcc ggcactttcc agggcgcgcg ccgccaggcc
     4921 ggcactttgg tgacagaccg gacagttggg caacagcagc gccgcatcga cgccatcggc
     4981 ctggcagcgc gtcaccagct ccgggcaatc gacctcgagg gtgacgcgat ggctgcggtt
     5041 tgtcggcagg ccgtgaaagc g
//
LOCUS       DASTMT010000272         5206 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_2115046, whole genome shotgun sequence.
ACCESSION   DASTMT010000272 DASTMT010000000
VERSION     DASTMT010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 5206)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5206)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5206
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2115046"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            22..840
                     /locus_tag="VFR00_06300"
     CDS             22..840
                     /locus_tag="VFR00_06300"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU0158901.1"
                     /translation="MRIIDAQIHLWRKGVVVPPHRTEPYALKEALADMDAAGVHGAIL
                     HPPSWDGDANAQAVEAARRYPERFAILGRIPLDRPDRRGEIESWREQPGMVGLRYTFL
                     QPHMKSWPMDGTMDWLWPVAERLGLPIALLAGEFLPLVGEIAARHPGLRLLIDHFGVA
                     RGNKDAAAFVTMPQVVALAKYPNIAVKVTGGPQYVSDGYPFKSLQPYYRAVYDAFGPR
                     RMFWGTDITRMPCSWRDCITHFSEHQPWLPAADRELIMGRAIADWLCWRPAWAK"
     gene            1000..2004
                     /locus_tag="VFR00_06305"
     CDS             1000..2004
                     /locus_tag="VFR00_06305"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0158902.1"
                     /translation="MVARPWAKALLLVAFALTSGAANAELNEIKITKQPGLLFTPMLL
                     MEQHKLVEKHARQAGLGDLKVAWVTIMSGGANNDALLSGSVHITTSGVTNMLLLWGKT
                     NGDVKAIVGVSGLPFKLLTRNPNIKTIKDYTASDRIAVPTVRQSIQAITLGIALQKAY
                     GDDKANEKLLANQVTMGHPDALTALLNPQHEVASHFASSPFQEIALKTPGIHVVLESR
                     DALGGDCHVALAYATSKFYEENPKTVRAFLAAYEEAVAMIKSDPKGSAQTYLSMVKER
                     ATAEEVEQLLTQPGAIFQAAPVRTMVYAEYMAKAGFIKPSPKSWKDYFFPLLHERDGS
                     "
     gene            complement(2038..3042)
                     /locus_tag="VFR00_06310"
     CDS             complement(2038..3042)
                     /locus_tag="VFR00_06310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0158903.1"
                     /translation="MVRSVATVVIAIALCLTAARPAGAEVAEVKLALQNGSNYLPLIV
                     MQHQKLVEKHLAAKGLAGTAVTWAKLAGPSAIIDSFLAGAVHFSGQGVPSTALIWDRT
                     RNGIGAKAISAMVASNIWLMTRKPELKSLRDLTDKDRIALPSIKTSAQALFLWTAAEK
                     EFGAGQWGKLDHLTVSLPHPDAMAAVLNPGGEITVHAATSPYADLEKKAGLKVITDLY
                     AVEGGAVTGLNFVSTEEFRKANPVTFAAVVAAFDEAIDWVNADKARAAQFYLEATKDK
                     ISLEDLTQILMAPDYIFGKTPHRVGAAIEIMHKAGILKTKPQSWKEMYFAEAHALPGD
                     "
     gene            3304..3576
                     /locus_tag="VFR00_06315"
     CDS             3304..3576
                     /locus_tag="VFR00_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006020314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4242 domain-containing protein"
                     /protein_id="HEU0158904.1"
                     /translation="MKRYVIEREIAGVGKLTGAELKGAAQQSNAALAKLAGKAQWLQS
                     YVAGDKTFCLYLAESEAAVHEHARLSGFPANKVTEISSIIDPTTAG"
     gene            3665..4726
                     /locus_tag="VFR00_06320"
     CDS             3665..4726
                     /locus_tag="VFR00_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006020313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU0158905.1"
                     /translation="MLDVSVILLSDNYASTAIGPIEVFHSAGLLWNTLNGTRPNPRFR
                     VTIASPDGDMVSSPYALRLMPQVPLRKVRHADIVIVPASGLDLDAQLARNAQLLPWLR
                     RKAAQGAHIAGICSGVVFLAAAGLLDGREATTHWAVASLFRQRYPKVDWHPEKFITED
                     RRMFCSGGVYASMDLSLYLVEKFCGHEVALQCAKALLINMPRLSQSGYATLPLSRPHT
                     DAKVRAAEHYLEKNYAADVSLELLAQDLGMSPRNFSRRFKRATGRLPGNYLQGLRVAI
                     AKEMLEDGARSVQVVSAAVGYEDAAFFRGLFKRSTGMTPGEYRENFAASNGTTGRALG
                     ATAGRRRAGGASAARSASP"
     gene            complement(4794..>5206)
                     /locus_tag="VFR00_06325"
     CDS             complement(4794..>5206)
                     /locus_tag="VFR00_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011472156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEU0158906.1"
                     /translation="TALGAKIARPDVPVVSVNGDGGFMFNVQELATAVQFRIGVVSIV
                     FNDNAYGNVRRMQQDLYGGRRIASDLKNPDFVRLAESFGAMALRATSPAELRGCLRRA
                     LKETGPVLIEVPIGETPAPWDFIQLPRLRPPRRT"
BASE COUNT          926 a         1699 c         1676 g          905 t
ORIGIN      
        1 gtttaggggt tagctcggcc aatgcggatc atcgacgcgc agatccatct atggcgcaag
       61 ggcgtcgtcg tgccgccaca ccgcaccgag ccctatgcgc tcaaagaggc gcttgccgat
      121 atggatgccg ccggggtgca cggcgccatc ctgcatccgc cgagctggga cggggatgcc
      181 aatgcccagg cggtggaggc cgcgcgtcgt tatcccgagc gttttgcgat cctcggtcgc
      241 attcccctcg atcgcccgga caggcgcggc gagatcgaga gctggcgcga gcagccgggc
      301 atggtcggcc tgcgctatac cttcctgcag ccgcacatga agtcgtggcc gatggacggt
      361 acgatggact ggctgtggcc ggtggcggag cgcctcggcc tgccgatcgc gctgctcgcg
      421 ggcgaatttc tgccgctcgt gggcgagatc gcggcccggc acccaggcct gcgtctgctg
      481 atcgatcact tcggcgtcgc ccgcggcaac aaggatgccg cggccttcgt gaccatgccc
      541 caggtcgtcg ccttggccaa atatcccaac attgcggtca aggtgaccgg cgggccgcaa
      601 tacgtgagcg acggctaccc gttcaaaagc ctgcaacctt actatcgtgc cgtctacgac
      661 gccttcggac cgaggcgcat gttctggggc accgacatca cgcgcatgcc ctgcagctgg
      721 cgcgactgca tcacgcattt cagcgaacac cagccctggc tcccggccgc cgatcgcgag
      781 ctcatcatgg gtcgggcgat cgccgactgg ctctgctgga ggccggcctg ggcaaagtga
      841 gggagcgcgc cagcggcggc cgcaaacttt cgttggccgc cgaggcggac gctctgcccc
      901 gccttcgcat ctgccccctg caggccaact tgtgtccggg caaacttgca agcaagctag
      961 caaggcaggc ccataagaaa ggcgaggagg acgtgatcca tggttgctag gccttgggcg
     1021 aaagccctgc tgctggtggc atttgcgctg acgagcgggg cggcgaacgc cgagctcaac
     1081 gaaatcaaga tcaccaagca gccgggcttg ctgttcaccc cgatgctgct gatggagcag
     1141 cataaactcg tggagaagca cgcgcgtcag gccggtcttg gcgatctcaa ggtcgcctgg
     1201 gtgacgatca tgagcggcgg cgccaataac gacgcgctgt tgtcggggag cgtgcacatc
     1261 accaccagcg gcgtcaccaa tatgctgctg ctgtggggga agaccaacgg cgacgtcaaa
     1321 gccatcgttg gggtcagtgg gctgcccttc aagctcctca cgcgcaatcc gaatatcaaa
     1381 accatcaagg actacacggc gagcgatcgg atcgcggtgc cgacggtgcg ccagtcgatc
     1441 caggcgatca ccctcggtat cgccctgcaa aaggcctacg gcgacgacaa ggccaacgaa
     1501 aagctgctcg ccaatcaggt gaccatgggc caccccgacg cgctgaccgc gctcctcaat
     1561 ccccagcacg aagtggcgag ccattttgct tcctcgccat ttcaagaaat tgccctcaag
     1621 acccccggta ttcacgtggt tttggaatcg cgcgacgcgc tgggcggcga ttgccacgtg
     1681 gcgctcgcct acgccaccag caaattctac gaggagaacc caaagacggt gcgcgccttc
     1741 ctggccgcct acgaggaggc cgtggccatg atcaagtccg atcctaaagg ctcggcgcaa
     1801 acctacctca gcatggtgaa ggagcgcgcg acggccgagg aggtcgaaca gctgttgacc
     1861 cagcccggtg cgatcttcca ggcagcaccg gtgcgcacca tggtctatgc cgaatacatg
     1921 gccaaggcgg gtttcatcaa gccgagcccc aagagctgga aggactactt ctttccgctg
     1981 ctgcacgagc gcgacggcag ttgagcggct ctttctgttt ggcgcctctt ggcgggccta
     2041 gtcgccgggc agggcgtgcg cctcggcgaa gtacatctct ttccaggact gcggcttggt
     2101 tttgaggatg ccggccttgt gcatgatttc gatcgccgcg ccgacccggt gcggcgtctt
     2161 gccaaaaatg tagtcgggcg ccatcaggat ctgcgtcaag tcctcgagcg agatcttgtc
     2221 cttggtagcc tcgaggtaga attgcgccgc gcgggcctta tccgcgttca cccagtcgat
     2281 ggcttcgtcg aatgcggcca ctacggcggc aaaggtgacg gggttggcct tgcggaactc
     2341 ttcggtggag acgaaattga gaccggtcac ggcgccgccc tccaccgcat aaagatcggt
     2401 gatcactttc agcccggcct tcttttccaa atcggcgtag ggcgaggtcg ccgcatgcac
     2461 ggtaatctct cccccgggat tgagcacggc ggccatggca tcggggtggg gcagcgatac
     2521 cgtcagatgg tcgagcttgc cccactgacc ggccccgaac tccttctccg ccgccgtcca
     2581 caggaacaag gcttgcgccg aggttttgat ggatggcagg gcgatgcggt ccttgtccgt
     2641 caaatcacgc agggatttga gctcgggttt gcgcgtcatc aaccagatgt tggaggcaac
     2701 catggccgaa atcgccttgg cgccgatgcc gttgcgcgtg cgatcccaga tcagcgcggt
     2761 cgagggcacg ccctgacccg agaaatgcac ggcacccgcc aagaaggaat cgatgatggc
     2821 ggatggcccc gccagttttg cccaggtgac ggcggtgccg gccagcccct tggcggccag
     2881 gtgcttctcc accagtttct ggtgttgcat cacgatcagc ggcagatagt tggagccgtt
     2941 ttgcagggcg agcttgactt ctgcgacttc ggcaccggcg ggacgggccg cggtgaggca
     3001 gagcgcgatg gcgatcacta ccgtggcaac gctgcgaacc atgtccacac ctcgtctgaa
     3061 gcttggcgct taggctttcc cgggctgtcc agcaccgcgc gccactgtgc ggcggggcgc
     3121 ggctgccagg caaagcggca atcggcctca gccgggacag gcaggctcca ccgcaagcga
     3181 gggacgctcg cggcttgtgt cgttttcacc tatgttcatt gtcagttccg ccactgggct
     3241 ggggaagctg ctctggtcta gacaggcgcc gcggcaaaaa ccacccgaga aagcgaggtt
     3301 gtcatgaagc ggtatgtcat cgaacgtgaa attgccgggg tcggtaagtt gacgggcgcc
     3361 gaactcaaag gcgccgcgca gcaatccaac gcggcgctgg caaaactcgc cggcaaggcg
     3421 cagtggctcc agtcctatgt cgccggcgac aagacctttt gcctttatct cgccgagagc
     3481 gaggccgccg tgcacgagca cgcgcggctc agtggatttc cggccaataa ggtgacggaa
     3541 atctccagca tcattgaccc aaccacggca ggctaagccg tgggcccgag gcggcgcgct
     3601 gccctgcatg ccgcccgcct tcgcgctagc cgcccgggct agcgcccaac ttatggatcg
     3661 gcccatgttg gacgtcagcg tcatcctgct cagcgacaac tacgcctcca cggcgatcgg
     3721 accgatcgag gtgttccaca gcgcgggtct gttgtggaac acgctcaatg gcacgcgccc
     3781 caatccgcgc ttccgagtga cgatcgcctc gcccgacggc gacatggtca gcagccccta
     3841 cgcgcttcgc ctcatgccgc aggtcccgct gcgcaaggtc cggcatgccg acatcgtcat
     3901 cgtacccgcc tccggccttg accttgacgc ccaactggcg cgcaacgccc agctgctgcc
     3961 ctggctccgg cgcaaggccg cgcagggcgc ccacatcgcc ggtatttgta gtggcgtcgt
     4021 ctttctggcg gcggccggtc tgctcgatgg ccgcgaggcg accacgcatt gggccgtcgc
     4081 ctcgcttttt cgccaacgct atcccaaggt cgattggcat ccggaaaaat tcatcaccga
     4141 ggaccggcgc atgttctgca gcggcggcgt ctatgcctcg atggacctga gcctctatct
     4201 ggtggagaaa ttctgcggcc acgaggtggc gttgcaatgc gccaaggccc tgctcatcaa
     4261 catgccgcgt ctctcgcaat ccggctacgc caccctgccg ctgtcgcgtc cgcacactga
     4321 tgcaaaggtg cgcgcggccg aacattacct cgagaagaac tacgccgctg acgtctcgct
     4381 cgagttgctg gcacaggatc ttggcatgag cccgcgcaat ttcagccggc gtttcaagcg
     4441 cgccaccggt cggctgcccg gcaactatct gcagggcctg cgcgtcgcca tcgccaagga
     4501 gatgctcgag gatggagcgc gctcggtgca ggtggtcagt gccgcggtcg gctacgagga
     4561 tgctgcgttc tttcgcggcc tcttcaagcg cagcaccggc atgacgcccg gcgaataccg
     4621 cgaaaacttc gctgccagca acggcaccac gggccgcgcc ctgggcgcaa cggcggggcg
     4681 ccggcgcgcg ggaggcgcgt cggccgcacg ttcggcctcc ccctgagccc ggcgcgcgcg
     4741 cctagtgtcc gtcgcgagcc aggggcgagc cggcagacga ggcagccaag ccgtcaggtg
     4801 cggcgcggtg gccgcagacg cggcagctgg atgaagtccc agggcgccgg ggtttcaccg
     4861 atcggcacct cgatgaggac cggtcccgtc tccttgaggg cgcgacgcag gcagccgcgc
     4921 agctcggctg gcgaggtggc gcgcaacgcc atggcgccaa aactttcggc caggcggacg
     4981 aaatcgggat ttttgaggtc gctcgcaata cggcgcccgc cgtagagatc ctgctgcatg
     5041 cggcgcacgt taccgtaggc gttgtcattg aagacaatgc tcaccacacc gatccggaac
     5101 tgcacggcgg tggcgagctc ctgcacattg aacatgaagc cgccgtcgcc gttgaccgag
     5161 accaccggca cgtcgggtcg cgcgatcttg gccccgagcg cggtcg
//
LOCUS       DASTMT010000273         4524 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_5981522, whole genome shotgun sequence.
ACCESSION   DASTMT010000273 DASTMT010000000
VERSION     DASTMT010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4524
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5981522"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..277
                     /locus_tag="VFR00_06330"
     CDS             <1..277
                     /locus_tag="VFR00_06330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0158907.1"
                     /translation="VRARCEQAGRDPAQLGISLLVQDPFEWSAHKTQDGRARRLFTGA
                     SADMVEDAHRLAVLGVGHVAVRLGGHSIEDAVARIERFGAEVIATLA"
     gene            complement(285..1430)
                     /locus_tag="VFR00_06335"
     CDS             complement(285..1430)
                     /locus_tag="VFR00_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012319384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEU0158908.1"
                     /translation="MPLATTRAPYWPAAEGGDWQRITPAEAGFDPAKLTIAINFAMAN
                     DSPWPHSLYYPDGRYVGIVEWNETGPWSEVAGPVTARGGPAGLILKAGRLVAEWGDTS
                     RVDMTFSVAKSYLAVLAGLAVADALIGAVDERVGAIVAGPYFASPHNASISWRHLLQQ
                     SSEWQGELWEKSDQVDHNRQIGAGADNSRKGVRRDLQAPGSYYEYNDVRVNLLALCLL
                     KRFGRALPEVLRDRVMDPIGASSGWQWHGYRTSWTDIEGQRVQSVSGGSHWGGGMFIS
                     ARDQARFGLLVARGGAWHGHQIIPSAWVTAMLSPSPSNESYGYLWWLNRGAARYPSAP
                     TSSVFALGAGSNIIWIDPEHDLVTVLRWINKAAVDGFLGRLLAAFGG"
     gene            1584..2546
                     /locus_tag="VFR00_06340"
     CDS             1584..2546
                     /locus_tag="VFR00_06340"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HEU0158909.1"
                     /translation="MARRINRAIELLGQDQAIYYVGHHTGHVLSWAQGRADSRTWADY
                     INIGMEHGAFDMAGLAEYVRGLIDGGPTNSGHRTPAVIVEVPVRGTDVASVHSNSWQF
                     RQILARGVHGLLLCQAETAAAVRAFLAAARYPHHLAGVDPGLPSALERMRGAGGFSAS
                     NDPPPVRACLAVGSRGRGSEATAAAAWGLSAADYMERCDPWPLNPGGELLLGVKIESP
                     EGVANCEAILEVPGLGFAEMGPGDLGLALGYRTVPRHPYPPEMQEARRRVFAACKRNG
                     VAFLEVGSPATITSQLDEGVRVIAGQDEGTARIGRAHQRRAMPV"
     gene            complement(2606..4120)
                     /locus_tag="VFR00_06345"
     CDS             complement(2606..4120)
                     /locus_tag="VFR00_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018272849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEU0158910.1"
                     /translation="MVALSSFIQFHARRTPDRLALIYGDDKITYARMLHRIETVAGWL
                     AAQGIGPGDVVALLMKNSPAFLELTFAASHLGAVSLPINYRLATDEVGYIFEDAGARL
                     LLCDQEFAATARGMPLLHLIDAAAQRDSARLASPAQSPARMHPSRPDDLFRLMYTSGT
                     TDRPKGVMHSYANFYWKSADHVIALGLSAADRLLVAGPLYHVGAFDLPGMAVLWVGGT
                     LSILRDFAPELALAAIAKERLTGAWLAPVMLGAILEHPERGAYNLESMRWVVGGGERT
                     PEGRIRAFSRLFARARYVDAYGLTESCSGDTMMEAGRELEKIGSVGRALAHVEVEVRG
                     PEGQRLAPGESGEICLRGPKITRGYWKDGQKTKAAFFDDWFRTGDVGQLDAEGFLYLT
                     DRKKDMIISGGENIASSEVERVVYELPQVQDAAVIGVPDDHWGERPVAVVVVKAGQAL
                     TLETLQAHCRHKLAGFKVPKQLIVRTDLPRNPSGKVLKRVLREQISRELEVRKP"
BASE COUNT          784 a         1493 c         1485 g          762 t
ORIGIN      
        1 agtgcgcgcc cggtgtgagc aggccgggcg cgatcccgcc cagctcggca tttcactgtt
       61 agtgcaagat ccatttgaat ggagcgcaca caaaacccag gacggcaggg cgcggcggct
      121 gttcaccggc gcttcggccg atatggtcga ggacgctcat cggctagccg tcctcggggt
      181 cggtcatgtt gccgtgaggc tcgggggaca ctcgatcgag gatgcggtgg cccgcatcga
      241 gcgcttcggc gctgaggtaa tcgccacgtt ggcctgaggc gaagctagcc gccgaaggcg
      301 gccaacagcc gccccagaaa gccgtcgacc gcggctttgt tgatccagcg taggacggtg
      361 acgagatcat gctcgggatc aatccagata atgtttgatc cagcccccag cgcaaacacg
      421 ctgctggtcg gcgcgcttgg gtagcgtgcg gcgccgcgat ttagccacca caaatagccg
      481 taactctcgt tgctgggcga gggggagagc atggccgtga cccaggccga gggtatgatc
      541 tggtggccat gccacgcccc gccgcgggcg accaggaggc cgaagcgggc ctgatcgcgt
      601 gccgagataa acatgccgcc accccagtgc gagccacccg acacggactg gacacgctgg
      661 ccttcgatgt cggtccacga cgtgcggtag ccgtgccact gccagcccga cgatgcgccg
      721 attggatcca tgacgcgatc gcgcaagacc tcggggagcg cccggccaaa acgcttgagc
      781 aggcagagcg ccaacaagtt gacgcgcacg tcgttgtact catagtagct gcccggcgcc
      841 tgcaggtcgc ggcgcacgcc cttgcggcta ttgtccgcac cggcgccgat ctggcggttg
      901 tggtcgacct gatccgattt ttcccacagc tccccttgcc attcgctgga ttgctgcaac
      961 aggtggcgcc agctaatcga cgcgttgtgc gggctggcga aatatgggcc agcaacaatg
     1021 gcgcccacgc gctcatcgac ggcgccaatt aaggcgtcgg cgacggcaag gccagcgaga
     1081 accgcgaggt agcttttggc aacgctaaac gtcatgtcca cccgtgaggt gtcgccccac
     1141 tcggcgacaa ggcggcccgc cttgaggatc aggcccgccg gccccccgcg cgccgtcacc
     1201 gggccggcaa cctcactcca tggccccgtt tcgttccact cgacgatgcc gacatagcgc
     1261 ccgtcgggat aatacaggct gtgcggccat ggactgtcgt tggccatggc gaaattgatg
     1321 gcgattgtca atttggccgg atcgaaaccc gcctcagccg gagtgatgcg ctgccaatcg
     1381 ccaccctcgg cggcgggcca atagggtgca cgggttgtcg ccaacggcat cgcgcgtctg
     1441 ctccccctgg tttttcttgc cgcgcgggga cttatcacgc ctagccctcg ccggcaaagc
     1501 gcctatgctg gcaggaaggt ggcgagccga cccagccggg cgcccctgcc tgcttgtggt
     1561 tcgaacacgg aaaatgacag cgcatggcac gccgaattaa tcgagccatt gaactcctgg
     1621 ggcaggatca agccatctac tacgtcggcc accatacggg gcacgtgctg agctgggcgc
     1681 agggccgagc ggattcccgg acctgggccg actacatcaa tatcggcatg gagcacggcg
     1741 ccttcgatat ggctggcctt gccgagtatg tgcgcggtct catcgacggc ggtccgacca
     1801 attccggtca tcgcacgccg gccgtcattg tcgaggtgcc cgtgcgcggt accgacgtcg
     1861 ccagcgtcca cagcaacagc tggcaattcc gccaaattct ggcccgcggt gtgcacggcc
     1921 tcttgttgtg tcaggccgaa acggcggcgg cggtgcgcgc ctttctggcg gcggcgcgct
     1981 atccacatca cctcgcaggg gtcgaccccg gcttgccgtc agcgctcgag cgcatgcgcg
     2041 gcgccggtgg cttctcggcg agcaacgatc ctccgcccgt gcgcgcctgc ctcgccgtcg
     2101 gcagccgcgg ccgcggctcg gaagcgaccg ccgccgccgc ttgggggctg tccgcggcgg
     2161 actatatgga gcgctgcgat ccctggccgc tcaacccggg gggagaattg ctgctgggcg
     2221 tcaagatcga gagcccggaa ggggtagcca attgcgaggc gatcctggag gtgcccggcc
     2281 ttggttttgc cgaaatggga ccaggcgatc tcggcttagc gctcggctat cgcactgtcc
     2341 cgaggcatcc ctatccgccc gagatgcaag aggcccgcag acgggtgttt gccgcctgca
     2401 agcgcaacgg tgtggccttt ctcgaggtcg gctcgccggc caccatcaca agccaattgg
     2461 acgaaggcgt ccgggtcatc gcaggacagg acgagggaac cgcccggatc ggccgcgcgc
     2521 accagcgtcg cgccatgccg gtctagcgcg aaagcattgc ctgcggctgt gatggctcgc
     2581 caggagccct cacgccggag caagctcagg gcttgcggac ttccagttcc cggctgattt
     2641 gctcgcgcag cacgcgcttt aggaccttgc cagatgggtt gcgcggcaaa tcggtgcgca
     2701 cgatcagctg ctttggcacc ttgaagccgg cgagcttgtg gcggcagtgg gcctgcaatg
     2761 tttccaacgt cagcgcctgg ccggccttga cgacaaccac cgctacgggg cgctcgcccc
     2821 agtggtcatc cgggacgcca atcaccgccg cgtcctggac ctgtggcagt tcgtagacaa
     2881 cccgctccac ctcggaggag gcaatgttct cgccacccga aatgatcatg tccttctttc
     2941 gatcggtgag atagaggaag ccttccgcgt ccagctggcc gacatcgccg gtgcggaacc
     3001 aatcgtcgaa aaaggcagcc tttgtcttct gcccatcttt ccagtacccg cgcgtgatct
     3061 taggaccgcg caggcagatt tccccgctct cgccgggcgc gagccgctgg ccctcgggcc
     3121 cgcgcacctc gacttccaca tgtgccagcg cacgaccgac cgagccaatc ttttcgagct
     3181 cgcggcccgc ttccatcata gtgtcgccgc tgcagctttc cgttaggcca taggcatcga
     3241 cgtagcgcgc ccgtgcaaac agtcgcgaga aggcgcggat gcggccttcg ggcgtgcgct
     3301 caccgccgcc gaccacccag cgcatgctct ccaggttata agccccgcgc tccgggtgct
     3361 cgaggatggc gcccagcatt acgggcgcga gccaggcgcc cgtaagacgc tctttggcga
     3421 tggcggccag cgctagctcg ggcgcaaaat cgcgtaggat tgacagcgtg ccgccgaccc
     3481 acagcaccgc catgccgggc aggtcgaagg cgcccacatg atagagcggg ccggcgacca
     3541 gcaaccggtc ggccgcgctt aggcccaaag caatgacgtg atcggcagac ttccagtaga
     3601 aattggcata gctatgcatc acgcccttcg gccggtcggt ggtgccggag gtgtacatta
     3661 ggcggaagag atcatcgggg cgcgaggggt gcatcctggc aggcgactgg gcgggcgagg
     3721 ccagtcgcgc gctgtccctc tgcgccgccg catcgatgag gtgcaagagc ggcatgcccc
     3781 tggcggtggc ggcaaactcc tggtcgcaga gcagcaggcg cgcgccggca tcctcgaaga
     3841 tatagccgac ctcgtcggtg gccagacgat agttgatggg cagcgatacg gcgccgagat
     3901 ggctcgccgc aaaagttagc tcgagaaacg ccgggctatt cttcatcagc agagcaacca
     3961 cgtcgcctgg cccgattcct tgcgccgcca gccaacccgc caccgtctcg atgcgatgca
     4021 acatccgcgc ataggtgatt ttgtcgtcgc cgtagatcag cgccaaccgg tccggcgtgc
     4081 gccgggcgtg gaactgaatg aagctcgaga gggcaaccat ggcctcagcc acccgaggcc
     4141 ttggcgtcgg ctgcgccggc acgggatcga tgcgcccgtc ttgcggccgc gccgccttga
     4201 agtcgacgtc gcatcgatct gcctcccaga agctcagagg tgacgccagg ctactccgcg
     4261 gagggtttct tgcccgccgg cggcggcgtc ggtcaaggct cggttttggg gaccggtcgg
     4321 gggactcaca gggggccacg gcatgaaatg caaagctgag gcaccatagg ccggccagct
     4381 gcatcgcgag ccccatggtg tgacgatggg tcggtcattc gctgtgccgc gctggcggac
     4441 actgtcacaa tgccctgcgc aagggacgtc aaaccgcgtt gcgcccgagt ttcctggccg
     4501 agcctgctgg caggatcaaa gggc
//
LOCUS       DASTMT010000274         2777 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_5653174, whole genome shotgun sequence.
ACCESSION   DASTMT010000274 DASTMT010000000
VERSION     DASTMT010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2777)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2777)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2777
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5653174"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            290..1546
                     /locus_tag="VFR00_06350"
     CDS             290..1546
                     /locus_tag="VFR00_06350"
                     /inference="COORDINATES: protein motif:HMM:TIGR01988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU0158911.1"
                     /translation="MSAGEEGRTEAQIAVVGAGLTGLTAAAALSQTGFDVVLAAPAFD
                     PGAAAADTRSSALLPPSVQLLENLNVWATCAPASAPLLGVRIIDDRGGLVRAPEVLFE
                     AREIGRPDLGANVPNAALSAALNAKLAASSQLRWLATTAVTAVEPDATAVQLRLAEGG
                     SIRAVLLVAADGRNSIARAAAGISARTWDYPQAAIATSFGHTRPHAGITTELHRRAGP
                     LTTVPLPGEASSLVWVEDPAQATQLAQLSEREFKATLEGRLQGFLGAIETLGPRARFP
                     LRGLNASTMARQRIALVGEAAHVIAPIGAQGLNLGLRDVATLNDCLAAARAAGRDLGG
                     IETLASYAQARAADIASRAAMVDLLNRSLLTDFFPAQALRGFGLHLLALSGALRRLAI
                     EGGVNAPGPLPRLMQPAQEPSGGRAV"
     gene            1636..2532
                     /locus_tag="VFR00_06355"
     CDS             1636..2532
                     /locus_tag="VFR00_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A biosynthesis acyltransferase"
                     /protein_id="HEU0158912.1"
                     /translation="MPARLKDLRYRIEYAALRLIVALVRAAPMDVSVAVSGKIWRLIA
                     PYDRRHKRALANLAIAFPDMALQKRETIARAMWENLGRVMAETMQIDRILADPRRIEI
                     ADEKMYLRYKDKLGSAIGVTLHMGNWELAIWPFTMAGNNPAAVYRTVKNPYVDRYIRN
                     IRKTLYPGGLLGRGRTRDEGQTTARLIMDFVRQGGRLGLVCDLHDRTGIPVPFFGQPA
                     RSVAIPAMIARRVGARIWMARCLRLGSESRFRIELKELKVPRTANPADDVRWITAEMQ
                     RQFETWVRQNPEQWMWSNRRWS"
     gene            2647..>2777
                     /locus_tag="VFR00_06360"
     CDS             2647..>2777
                     /locus_tag="VFR00_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HEU0158913.1"
                     /translation="MSATRTPAKHFTALAVGAPEPYRALPVRLERMIHFVPPHNEKIR
                     "
BASE COUNT          467 a          973 c          872 g          465 t
ORIGIN      
        1 ccacacgggc cggatggtga tgggttgcga ttgccgtcaa gggccgcccg tccgtgttct
       61 gcaaggctta acgcactcgt taatgagcgg taaacaagca atgcacagtt acccaagctt
      121 tccacctagc acgtggcgta aaagccacga tacgattcga gtcggtgctt gaattctggg
      181 gcatttctgg cacagcgatt ctattgtcaa cggctttatg tgacaggctc ttccgctgcc
      241 tgtgagtata aggggtagcc attgcggtcg ctcagaaaag aggcagagaa tgagcgcagg
      301 cgaggagggc agaactgagg cacaaattgc cgtcgtgggc gcgggactga caggtctcac
      361 cgccgccgcc gcactcagcc aaacgggctt cgacgtcgtg ctggcagcgc ccgccttcga
      421 cccaggagcc gcggccgccg acacgcgctc ctccgccctc ctcccgccga gcgtgcaact
      481 ccttgaaaac ctgaacgtct gggcaacatg cgcaccggcg agcgcgcccc tcctgggcgt
      541 gcgcattatc gacgaccgcg gcgggctcgt gcgggcgccg gaagtgctgt tcgaggctcg
      601 cgagatcggc cgccccgacc tcggcgccaa cgtgcccaac gccgcgctga gcgcggcgct
      661 taacgccaag ctggctgcct cctcgcagct gcgctggctc gccaccacgg cggtgacggc
      721 cgttgaaccc gacgcgaccg ccgtccagct gcgcctcgcc gaaggcggct cgatccgcgc
      781 ggtcctcctg gtcgctgccg acggccgcaa ttcgatcgcc cgcgcggccg cgggcatctc
      841 tgcccgcacc tgggattatc cacaggcggc gatcgccacc agctttggcc acacccgccc
      901 gcatgccggc atcaccaccg aactgcaccg acgtgccggt cccctgacca ccgtgccgct
      961 gcccggggag gcctcgagcc tggtgtgggt ggaagatcca gcccaagcga cgcaattggc
     1021 tcagctgtcg gagcgggagt tcaaggcgac actggagggg cgattgcagg gttttctcgg
     1081 cgccatcgag acgctcggtc cccgcgcccg ctttccgctg cggggtctca atgcctcgac
     1141 gatggcacgc cagcgcatcg cgctcgtcgg cgaggccgcg catgtcattg cgccgattgg
     1201 cgcccagggg ctcaatcttg gtctgcgcga cgtcgccacg ctcaatgact gcctggcggc
     1261 tgcccgagcc gccggccgcg atctcggcgg cattgagacg cttgccagct acgctcaagc
     1321 ccgtgcggcc gacatcgcca gccgtgcggc catggtcgat ctcctcaacc gctcgctcct
     1381 gaccgacttt tttcccgccc aggcgctccg gggcttcggc ctacacctgc tggcgctgtc
     1441 gggcgcgctg cggcgcttgg cgatcgaggg cggggtcaat gcgccgggac ccctgccccg
     1501 gctgatgcag cccgcgcagg agccctccgg aggccgcgcc gtctagcgcg ggagggcggt
     1561 tcgggaggtc ttgacgccgc gagcctcaca aggcaagtgc gcttgcctgc cccgccgcaa
     1621 cagccaaatc ttttgatgcc agcccgcctg aaggatctcc gctaccggat tgagtacgcc
     1681 gcgctgcgtc tcatcgtggc gctggtacgg gcggccccca tggacgtctc cgttgccgtt
     1741 tccggcaaaa tttggcgcct gattgctccc tacgaccgcc gccacaagcg cgcgctggcc
     1801 aatctcgcga tcgcttttcc cgacatggcc ctgcagaagc gcgaaaccat cgcccgcgcc
     1861 atgtgggaaa acctcggccg cgtcatggcc gagacgatgc agatcgaccg catcctcgcc
     1921 gatccccggc gcatcgaaat tgccgacgaa aagatgtatc tgcgctacaa agacaagctc
     1981 ggctcggcca tcggcgtcac cttgcacatg ggcaattggg agctcgccat ttggcctttc
     2041 accatggccg gcaacaaccc cgctgcggtc taccgcaccg tcaagaaccc ctacgtcgat
     2101 cgctacatcc gcaacattcg caagacgctc tatcccggtg gactgttggg cagaggacgc
     2161 acgcgcgacg aaggccagac cacggcgcgg ctgatcatgg acttcgtccg ccagggtggg
     2221 cggctgggat tggtctgtga cctgcacgac cgcacgggca tccccgtgcc ctttttcggc
     2281 cagccggcaa ggtcggtcgc catccccgcc atgatcgcgc gccgcgtcgg cgcgcgcatc
     2341 tggatggcgc gctgcctgcg cttgggttcg gagagccgct tccgcatcga gctcaaggaa
     2401 ttaaaggtcc cgcgcaccgc caacccggcc gatgacgtgc gctggatcac cgcggaaatg
     2461 caacggcagt tcgaaacctg ggtgcgccag aacccggagc agtggatgtg gagcaaccgc
     2521 cgctggagct gagcctcctc gcgtcgaagg catgctaagg cgcgcgaaca cacgccctcc
     2581 cgccggcgtt tgactcttga ggaccggggc ctagagtgcc ctaccgcagc gcaacgaaag
     2641 tgagccatgt ctgcgacgcg cacgcctgcg aaacacttta ccgcgttggc tgtcggcgcg
     2701 ccggagccct accgggccct gccggtacgg ctcgaacgca tgatccattt tgtgcctcca
     2761 cataatgaaa agatccg
//
LOCUS       DASTMT010000275         5311 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_4669246, whole genome shotgun sequence.
ACCESSION   DASTMT010000275 DASTMT010000000
VERSION     DASTMT010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 5311)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5311)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5311
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4669246"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..198
                     /locus_tag="VFR00_06365"
     CDS             <1..198
                     /locus_tag="VFR00_06365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HEU0158914.1"
                     /translation="AALVYKRRRHVINVFMWPADGEPRAAPKATALQGYNTLSWTAGG
                     ITFWAVSDLNAKELGELQGLL"
     gene            709..960
                     /locus_tag="VFR00_06370"
     CDS             709..960
                     /locus_tag="VFR00_06370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0158915.1"
                     /translation="MKRTSLNRIYAKTNPARVFDSPSEIVDELLLTRGEKLGTLDRWR
                     RSILDQLDTHDQERRGYAAFSQLLGQIEEAKLRLSTSLP"
     gene            complement(1096..2052)
                     /locus_tag="VFR00_06375"
     CDS             complement(1096..2052)
                     /locus_tag="VFR00_06375"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEU0158916.1"
                     /translation="MRHIANILAALVIWLGLGAGARAQDAAKYPEQMVRLVVPFSAGS
                     MTDLLARVVAEKMQQRWGKDVVVENRPGLAGTASVAKAPADGYTLMLSSNGHTVIGHL
                     NKNLAFDPVRDFVGVTQVATTPLILAVPPDSSPKTVKDLVELARSKPGELNYSSAGLG
                     STTGIAGALFKQTTKTDIVHLPFKGLPETHTAIIRGDVALGFSFFSAAGDLIQGGRMR
                     ALAVTGPERLGVLPEVPTFKEAGLPEFQYDSWFGILAPAGTPKAIIDKASRDIAEIVR
                     MPDVKARFEPQGAILVSTSPERFDEILRDDVQRYAPLFKQTN"
     gene            complement(2415..2771)
                     /locus_tag="VFR00_06380"
     CDS             complement(2415..2771)
                     /locus_tag="VFR00_06380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0158917.1"
                     /translation="MTTTLVEQIEEMRVRMNQLARREQDLVRALGQALNRADQKLLQD
                     VRNVALEHEVRREGILRELQTLATRMGALPMPRQPYASLENAPIDVPSYEANQPAPRT
                     GDWRQAAANLREHLPN"
     gene            complement(3294..4352)
                     /locus_tag="VFR00_06385"
     CDS             complement(3294..4352)
                     /locus_tag="VFR00_06385"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU0158918.1"
                     /translation="MSQWNKYGATAARAHGRPGRELRGKSLTIDLHSHVGIARASEFV
                     RPHLSSTASPLVRYANAETNALNQKQEADIVARAGLDQRLADLDAMGLDMQVIKPPPP
                     QCYYALPVEIAVAAARIVNDGIAEFVARKPDRLKGFGTVPMPDGNEAAKELERCVTEL
                     GFKGVQILTNVNGEELSDPKFAPFWRKAEELGVLVVIHPNGFTDAGRLSRFYFNNVIG
                     NPLETSIAVHYLIFDGVLERHPKLKILAVHGGGYLPSYAGRIDHAWGARSDSHGTLPQ
                     PPTTYLKRLYFDSIVFTPHQLQELVRLYGVEHIVMGTDYPFDMADYDPVGHVAACGFD
                     AATMAAICGGNAKALLGL"
     gene            complement(4427..5146)
                     /locus_tag="VFR00_06390"
     CDS             complement(4427..5146)
                     /locus_tag="VFR00_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018494358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0158919.1"
                     /translation="MASEALALRDIDAYYGDSHALQQVSFTAGAGRLLGLVGRNGAGK
                     TTAINVAVGLLPPRAGRVEVFGTNVAGFTPEAIAACGVALVPQGRRVFRSLTVRENLS
                     VAQRKPIAAGKTRWDLDTVFQMFPRLAERRHQLASLLSGGEQQMLAIGRALMGNPRLL
                     LMDEPSEGLAPQIVTDVGRTIARLKGEGLSIVLVEQNIKLTLDLADDIVILNSGHVAF
                     TGLAGQIALDDAIVAQHLGVH"
BASE COUNT          996 a         1735 c         1663 g          917 t
ORIGIN      
        1 gccgccttgg tctacaagcg ccgtcgccac gtcatcaacg ttttcatgtg gcccgccgac
       61 ggcgaaccgc gtgcggcgcc aaaggccacc gccctgcagg gctacaacac gctgagctgg
      121 accgccgggg gcataacctt ctgggccgta tccgatctca acgccaaaga gttgggcgaa
      181 ctgcagggcc tgctttagcc gcattgtcgt aacgcgaaag cgccggtcgg tctgccccgg
      241 cgctcgcgac tgaggcgccc ggccaacacg ggaccgggcg caaggggcgg cgcttagtgc
      301 gcggcgtaaa gcggtgactc cgtggcgagc acggcgccaa ccaccggcat cccacttttc
      361 tcgttttgga caataagacc gaccagcgtt tcgcgttcgg cctttagggc ttccatacgt
      421 acgaaccggc tttcacccaa tttgtttcta accaatccac tgtactcgat attgacgtct
      481 ctcaaccgct ttcggattgc aattaagtcc gtgcgagtca tggtccacct tccctcgatt
      541 ggtcccacac ccccgcatga ttcgtgtgac acgatcgcgg cgcggaaacg gccgattgga
      601 tcgttagcgt acgctgcgaa agattttttt tcgcacgcag atgcggcgtg cacacattaa
      661 tgccgcgaat caatgaggaa atcccgatgc gggcttcggg aggaaagcgt gaaacgaacg
      721 tccttgaacc gcatctacgc caaaacaaat ccagcacgcg tattcgatag cccctccgag
      781 atcgtcgacg aactactcct cacgcgcggc gagaagctcg ggactttgga tcgctggcgt
      841 cgaagcatcc ttgatcaatt ggacacccac gaccaggaga ggcgaggcta cgccgccttt
      901 tctcaactgt tgggacagat cgaggaagcc aagctgcgcc tcagtaccag cctgccatag
      961 ggttctatca cccctcgcaa caatggctgc tggccgagaa agtggcggcc cgctgccact
     1021 ttcttttgac atcggggcgc tcgactgagg tcccccgcgg cacgcacgcc gctaccggcg
     1081 ctcggccata tcggcttagt tcgtttgctt gaacagcggt gcataacgct gcacgtcatc
     1141 cctgaggatc tcgtcaaagc gctcgggcga cgtgctgacg aggatcgcac cttgcggctc
     1201 aaaacgcgcc ttgacgtccg gcatgcgcac gatttcggca atgtcgcgac tggctttgtc
     1261 gatgatcgcc ttcggcgtgc cggcgggcgc caagatgcca aaccaggagt cgtattgaaa
     1321 ctcgggcagc cccgcctcct tgaaggtcgg cacctcaggc aatacgccca agcgctccgg
     1381 gcccgtgacg gccagcgccc gcatgcgtcc gccctgaatg agatcgccgg ccgccgaaaa
     1441 aaacgaaaac ccgagcgcaa cgtcgccgcg aataatggcc gtatgggttt cgggcagacc
     1501 cttgaacggc aggtgcacga tatccgtctt ggtcgtttgc ttaaagagcg ccccggcgat
     1561 gccggtcgtg ctccccagcc ccgccgagct gtaattcagt tcaccgggct tcgagcgcgc
     1621 cagctcgacc aagtctttga ccgtcttggg cgaggagtcc ggcggcaccg ccaggatcag
     1681 cggcgtggtg gccacctgcg tcacgcccac gaaatcgcgg acgggatcga acgccagatt
     1741 cttgttgagg tgaccgatga cggtgtgtcc gttggaggac aacatcagcg tgtagccgtc
     1801 cgcgggcgcc ttggcgacgc tggcggtccc ggccaatccc ggccggttct ccaccaccac
     1861 gtccttgccc cagcgctgtt gcattttctc ggcaaccacg cgggcgagca ggtcggtcat
     1921 actgccggcc gagaagggca cgaccaggcg caccatttgt tctggatact tggcggcatc
     1981 ctgggcccgc gccccggcgc cgagccccaa ccaaataacg agggcggcaa ggatattggc
     2041 tatgtggcgc attgaaacgt cccttctcaa atccacccgt tgcgccacgg cccgcagggg
     2101 cgcgccggga gcgcgatctc gcgagcgcga cccgctctgg gcggagccgc cacctgagcc
     2161 gctgagccaa gtccatgaga cgagcgccgc gcgcgaggcg catgctaacc ggtcttaggc
     2221 ggcttcgaaa gcgcccgacg gccacaaggc aagggccggg agcccagcgc cggcgaaccc
     2281 gaaacatcgt ggaggaggcg cgccggccgc gggatcggcc gtgccgcgcg gtctcgcttg
     2341 ggtggccttg gggtggcctt ggggtggccc tgaaggggca ccgggcgccg ggggaggcgc
     2401 ccgtgatgcg cttatcagtt gggtaggtgt tcgcgcagat tggcggcggc ctgccgccag
     2461 tcgcccgtgc gcggcgccgg ctgattggcc tcgtagctcg gcacgtcgat aggcgcattc
     2521 tccagggagg catagggttg cctcggcatt ggcaaggccc ccatgcgcgt tgccagggtt
     2581 tgcaattccc tcaggatgcc ctcgcgccgc acctcgtgct cgagggcgac attgcgcacg
     2641 tcctgcagga gcttctggtc ggcgcgattg agcgcctgcc ccagcgctcg caccaggtct
     2701 tgctccctgc gcgcgagctg gttcatgcga acgcgcattt cctcgatctg ctcgacgagt
     2761 gtcgtcgtca ttgcacattt ccctcttttg tcggtcgcaa tggcccgtga cgaatcactg
     2821 gggcaaaagc atccgtaacg agtttggtca tgcagaggcg ggcgccaagg atacgaaaca
     2881 gggcaccctg cgcgcaccca tcgggcccac gccactcgag ctcatcgtcg tcgatccgca
     2941 aaccgggcta tcccctttgc gccaaaaggc tcgtgccacc aagccgtcct cgatgaggac
     3001 gcgcgcagca gcgagcacgc acgcgatgac actggagaga ctgcagggac tggaactgga
     3061 gaaaggactg gaactggaga aaggactgga acgcgggagg cggaactcac cgacagccgc
     3121 aaaccccgca acgcgacaaa ccgagcctga aacaggtcgg ccaaccgaga caacggctga
     3181 ggcggcacaa cattcccgcg tcgccgaaat gttctccgcg cgcggcacgc ccgcaaagcg
     3241 ccccgcggcc gcaaacgatt tcgccgcggg ctggggctac ggtgcagcga acgctagagg
     3301 cccagcagcg ccttggcgtt gccgccacaa atggccgcca tggttgcagc atcaaagcca
     3361 caggcggcga cgtgaccgac cggatcgtaa tcggccatgt cgaagggata atccgttccc
     3421 atgacgatat gctcgacgcc ataaaggcgc acgagttcct gcagctggtg cggggtgaag
     3481 acgatgctat cgaaatagag gcgtttcaga tacgtggtcg gcggctgtgg cagggtccca
     3541 tgcgaatcgg agcgcgcgcc ccaggcatgg tcgatgcgcc ccgcgtagga cggcaaatag
     3601 ccgccgccgt gcaccgccaa aatcttgagc ttcgggtgcc gttccagcac gccgtcgaag
     3661 atcaggtagt gcacggcaat gctggtctcc aagggattgc cgatcacatt gttgaagtaa
     3721 aaacgcgaca gccgaccggc gtcggtgaag ccgttggggt ggatgaccac cagcacgccg
     3781 agctcctccg ccttgcgcca gaacggcgca aacttcggat ccgatagctc ctcgccattg
     3841 acgttggtga ggatctgcac gcccttaaaa cccagctcag tgacgcagcg ctcgagctct
     3901 ttggcggcct cgttgccgtc cggcatcggc acggtgccga aacccttgag gcggtccggt
     3961 ttgcgggcca cgaactcggc gatgccgtcg ttgacgattc gcgccgccgc aacggcaatc
     4021 tcaacgggca gcgcgtagta gcactgcggc ggcggcggtt tgataacctg catgtccagc
     4081 cccatggcgt cgaggtcggc aagacgctga tcgagaccgg cgcgcgccac gatgtcggct
     4141 tcctgctttt gattgagcgc attcgtctcg gcgttggcat agcgcaccaa cggcgaggcc
     4201 gtggacgaca ggtgcggccg cacgaactcg ctggcgcgcg cgatccccac gtgcgagtgc
     4261 aaatcaatgg tcaggctctt tccccgcaac tcgcgcccgg gcctgccgtg cgcccgcgcc
     4321 gccgtcgccc cgtacttgtt ccattggctc atgcaatcct cctgacatca ccgctgccgt
     4381 ggttggcacc ccggcgcgta aagtcgcagc ccgtgcctgt gccccctcaa tgcacaccca
     4441 ggtgctgggc gacgatggca tcgtccaaag caatctgccc ggccaagccg gtgaacgcca
     4501 cgtggcccga gttcagaatg acaatgtcgt cggcgaggtc cagcgtcagc ttgatgttct
     4561 gttccaccag cacgattgag agcccttcgc ccttgagcct ggcaatggtg cggccgacat
     4621 cggtaacgat ctgcggggcc agtccctcgg atggctcatc catcagcaat agccgcggat
     4681 tgcccatcaa cgcccgacca atcgccagca tctgctgctc gccgccggag agtaggctgg
     4741 cgagctgatg ccgccgctcg gcaaggcgcg gaaacatttg aaataccgta tcgaggtccc
     4801 agcgtgtctt gccggcggcg atcggtttgc gttgcgccac gctcaaattc tcgcgcacgg
     4861 tcaagctgcg gaacacgcgg cggccctgcg gcaccagcgc cacgccgcag gcggcgatgg
     4921 cctccggcgt gaaacccgcc acattggtgc cgaagacctc gacgcggccg gcgcgtggtg
     4981 gcaagaggcc gacggcgacg ttgatggcgg tggtcttgcc cgcgccattg cggccaacca
     5041 ggccgagtaa gcgcccggca ccggcggtga acgacacctg ctgcagggca tgactgtcgc
     5101 cgtagtaggc gtcgatatcg cgtaatgcga gcgcctcaga cgccaaggta cacctcgcgt
     5161 gtgcgtgggt cggccaccac cgccgcacgc gtgccctcga cgatcacttc gccgaaatgt
     5221 aacagcgtaa tgcggtctgc aagctcgagg gcaacatcca tattgtgctc gatcatcacc
     5281 accgtgacgt cgcgaggaat ggcgagcagc a
//
LOCUS       DASTMT010000276         3978 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_2116078, whole genome shotgun sequence.
ACCESSION   DASTMT010000276 DASTMT010000000
VERSION     DASTMT010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3978)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3978)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3978
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2116078"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..507
                     /locus_tag="VFR00_06395"
     CDS             <1..507
                     /locus_tag="VFR00_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015668354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU0158920.1"
                     /translation="ALFPWKTVLGNVMYGLLEQGVAKPLAEQKARSLIALVHLNGYED
                     FYPKQLSGGMKQRVAIARTLAYDPKILLMDEPFGALDAHTRTGLQRELLEIWERARKT
                     VLFVTHSVEEAVFLSDRVIVLTRSPGRLKATIGIDLPRPRQRAELLVDRRYQTLVVEI
                     ERLMDTPG"
     gene            516..1301
                     /locus_tag="VFR00_06400"
     CDS             516..1301
                     /locus_tag="VFR00_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007601568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEU0158921.1"
                     /translation="MHARASSPSGFAPLFAAIGLLAMWEVAARCLGITGLPPATDALR
                     ELPAILGDAEALLNIAASLRRMLTGFVLALLLAVPVGLCMGRSERVAAAFNPALMIIY
                     PVPKAALMPIIMLWLGIGDASKTIVITLGVSLPVVYHSYQGARAVEEKLIWSAAAMGM
                     GRAARLWRVVLPAALPQVFVGIRTGLVLALITMVTSEMIARQTGVGSILFNALDMAQY
                     TTVYAMIVIIGLLGFVLDVLIEKLRYSLVQWAEPAHEIAVGTA"
     gene            1298..2065
                     /locus_tag="VFR00_06405"
     CDS             1298..2065
                     /locus_tag="VFR00_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018643131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEU0158922.1"
                     /translation="MRAERPWLLVLGALPLACLLALWQGVAAAGLAPAALLPAPAAVF
                     ARLLEQAHNPIFLAHAATTLLRLFAGFGIALLVGVTLGLGAATNRWAEALIRPLWRVL
                     APVPKIALYPALVLLLGFEHASKIALVVADAVFPILLATYQGTAAVEPKLVWAARAAG
                     AGRHRALLTVVLPAALPMILTGARVALIISAIVVFLAEMLSSTDGLGHLLVYASRSFQ
                     TVDMFVPLITISIIGLLLNAGFNGLRKRLLAGFPEEG"
     gene            2132..3178
                     /locus_tag="VFR00_06410"
     CDS             2132..3178
                     /locus_tag="VFR00_06410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018454833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M29"
                     /protein_id="HEU0158923.1"
                     /translation="MFADRIEGKWIDAFCAIFERCALKPGDCAAILSETQSRQLNVQL
                     AELALLRLGAKPFHVVVPTPRNTHPVPVRSTGASEAIGKLVPVVTALAQAGFVVDCTI
                     EGLMHAPETPAILKAGARILVISNEHPEALERMFPDAALERRVRAAAKMLRGAKRMTV
                     TSAAGTNLAIDMAGASTVGVWGWTDRPGTLAHWPGGLVVSFCRSGSVNGTLVLDRGDV
                     NLTFKRYLESPLRLTLEADYVREIEGVGADAEMMRSYLAAWGEREAYAVSHVGFGLNP
                     KARYEALSMYDRQHFNGTELRAVAGNFLFSTGANEFAGRYTAGHFDIPVMRTTIALDG
                     TEIIRAGVLSEVFG"
     gene            complement(3185..>3978)
                     /locus_tag="VFR00_06415"
     CDS             complement(3185..>3978)
                     /locus_tag="VFR00_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009026175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HEU0158924.1"
                     /translation="GLLPPSAVDGILARVSVLPEREAAAPMAQAAVVFEAVPELPELK
                     RQVLGRLSPLAPDSIIASTTSTILVDALADAVFKPERFLNAHWLNPAFLVPLVELSPG
                     RTTEPGVTQRLKALLESIGKVPVVCAARPGYIVPRIQALAMNEAARLVEEGVASAEDI
                     DKAIKYGFGVRFATLGMLEFIDWGGGDILYYASRYLTAALASERYAAPEIVERNMREN
                     RVGLKTGKGFLNYETLDVEAYRRQRLESFVKLLGHLGLTRPPVLP"
BASE COUNT          628 a         1341 c         1266 g          743 t
ORIGIN      
        1 gcgctgtttc cctggaagac cgtgctcggc aacgtcatgt acggcctgct cgaacagggg
       61 gtcgcaaaac cgcttgccga gcagaaagcc cgttccttga tcgcgctcgt gcaccttaac
      121 ggctatgagg atttctaccc caaacaactt tcggggggca tgaaacagcg cgtcgccatc
      181 gcccgcacgc tggcctatga tccaaaaatc ctgctgatgg acgaaccgtt cggcgcgctc
      241 gatgcccaca cgcgcaccgg tctgcagcgc gagctgctcg agatctggga gcgcgcccgc
      301 aagaccgtat tattcgtcac gcacagcgtg gaggaggcgg tgttcctgtc cgaccgcgtg
      361 atcgtgctca cgcgctctcc cggccgcctc aaggccacta tcggcatcga cttgccgcgt
      421 ccacgccagc gcgccgaact gctggtcgat cggcgttatc aaacgttggt cgtcgaaatc
      481 gagcggctga tggatacacc aggttaggca cgggcatgca cgcgcgggct tcctccccct
      541 cgggttttgc gccgttgttc gccgcaatcg gcctgttggc gatgtgggag gtggcggcgc
      601 gctgccttgg cattaccggg ctgccgccgg ccactgacgc cctgcgcgag ctgcccgcca
      661 tcctcggcga tgccgaggcg ctcctcaata tcgccgcctc gctgcggcgt atgctgacgg
      721 gctttgtgct ggctttgctg cttgcggtgc ctgttggcct gtgcatgggg cgaagcgaaa
      781 gggtcgccgc cgccttcaac ccggccttga tgatcatcta ccccgttccc aaggccgccc
      841 tgatgcccat catcatgctg tggcttggca tcggcgatgc gtccaagacg attgtcatca
      901 ccttgggcgt gagcctgccg gtcgtctacc acagctatca gggcgcgcgt gccgtcgagg
      961 agaagctgat ctggtcggcg gcggccatgg gcatgggcag ggcggcccgg ctttggcgcg
     1021 ttgtcctgcc cgccgccttg ccacaagtct tcgtcggcat tcgcacgggc ttggtgttgg
     1081 cgctcatcac catggtgacg agcgagatga tcgcgcgcca aaccggcgtc ggcagcatcc
     1141 tattcaatgc cctcgatatg gcgcagtaca ccactgtcta tgccatgatc gtcatcatcg
     1201 gcctcttggg ttttgtgctc gacgtgctga ttgaaaaact gcgctatagc ctcgtgcaat
     1261 gggccgaacc cgcccacgaa atcgccgtag ggaccgcttg agggccgagc gtccatggct
     1321 gctggtattg ggcgcgctgc cgcttgcctg cctgctggcg ctttggcagg gcgttgccgc
     1381 cgccggcttg gcgcccgcgg ccttgctgcc ggcgccggcc gccgtgtttg cacgtctcct
     1441 cgagcaggcg cacaatccca tcttcctcgc gcatgcggcc accacgctgt tgcgcctatt
     1501 tgccggcttc ggcattgcgc tcctcgtcgg cgtgacgttg ggcctcggcg ccgcgaccaa
     1561 ccgttgggcc gaggcgctga tcaggccgct ttggcgcgta ctggcgccgg tgccaaaaat
     1621 tgccctttac cccgccctgg ttttgctcct cggcttcgag catgcctcca agatcgcgct
     1681 ggttgttgcc gacgccgtgt tcccgatcct gcttgccacc tatcaaggaa ctgcagccgt
     1741 cgagccgaag ctggtctggg ctgcccgcgc ggccggcgcc gggcgccaca gggcgttgct
     1801 gaccgtggtg ctgcccgccg ccctgccgat gatcctgact ggcgcccgcg tcgccctcat
     1861 catctcggcc atcgtcgtct tcctcgcgga aatgctgagc tcgacggacg ggctgggcca
     1921 tcttctcgtc tatgcctcgc gcagctttca aaccgtcgac atgttcgtcc ccctgatcac
     1981 catctcgatc atcggcctcc tgctcaacgc cggcttcaat ggcctgcgca agcggctgct
     2041 ggcgggcttc ccggaggagg ggtgaagcgt tgcccacgcc ccgcgctcct gcctatgatt
     2101 gccgccgctg gcgttaaccc aagagggcgc gatgttcgcc gaccggatcg aaggcaaatg
     2161 gattgacgcg ttctgcgcca tcttcgagcg ctgcgcgctg aaacccggtg actgcgcggc
     2221 catcctgtcg gagacgcaat cgcggcagct gaacgtccag ctcgccgagc tggcgctgct
     2281 gcggctcggc gccaagccgt ttcacgtggt ggtgccgacc ccgcgcaata cccacccggt
     2341 tcccgtgcgc tcgaccggcg ccagcgaggc gatcggcaag ctggtcccgg tcgtgacggc
     2401 gctggcgcag gcgggcttcg tggtcgattg caccatcgag ggcctgatgc acgccccgga
     2461 aacgccggcc atcctgaagg ccggcgcccg catcctggtc atctccaacg agcatcccga
     2521 agcgctcgag cgcatgtttc ccgatgccgc gctcgaaagg cgcgtgcggg cggcggcgaa
     2581 gatgctgcgc ggcgccaaac gcatgacggt cacttcggcc gccggcacca atttggctat
     2641 cgacatggcc ggtgcctcca cggtcggcgt ctggggttgg accgatcggc cgggcacgct
     2701 ggcgcattgg ccaggcggcc tcgtcgtctc cttctgccgc agcggcagcg tcaatggcac
     2761 cttggtgctc gaccgcggcg acgtcaattt gaccttcaaa cgctacctgg agagcccgct
     2821 gcggctgacg ctagaggccg attacgtgcg cgagatcgag ggggtcggcg ccgacgcgga
     2881 gatgatgcgc agctaccttg ccgcctgggg agagcgcgag gcctatgccg tcagtcatgt
     2941 tggctttggc ctcaatccga aggcgcgtta cgaggcgctc agcatgtacg accgccagca
     3001 cttcaacggc acggaattgc gcgccgtggc cggaaatttc ctattttcga cgggagccaa
     3061 cgagttcgcc ggccgctata ccgccggcca tttcgacatc cccgtcatgc gcaccacgat
     3121 cgcgctggac ggcaccgaga tcatcagggc tggcgtgctg agcgaggtat tcggttgagc
     3181 caaatcaagg caacaccggc ggccgcgtca ggccaagatg gccgagcagc ttgacgaagc
     3241 tttcgagccg ctgccggcgg taggcctcca cgtccagcgt ctcgtagttc aagaaacctt
     3301 tgcccgtctt cagcccaacg cggttctcgc gcatgttgcg ttcgacgatt tcgggtgccg
     3361 cgtagcgctc gctagcgagc gcggcggtca gatagcgact ggcgtaataa agtatatcgc
     3421 caccgcccca gtcgatgaat tcgagcatgc ccaaggtggc gaaacgcacg ccaaaaccgt
     3481 atttgatggc cttgtcgatg tcctcggcgc tggcaacccc ctcctcgaca agccgcgcgg
     3541 cctcgttcat cgccaaagcc tgaatgcgcg gcacgatata tccggggcgc gccgcacaga
     3601 cgacggggac cttgccaatg ctctccagca gcgccttcag gcgctgagtg acgccgggct
     3661 cggtggtccg accgggcgaa agctccacca gcggcaccag aaacgccgga ttgagccaat
     3721 gcgcattgag aaagcgctcg ggcttaaaaa cggcgtcggc cagagcatcg accaggatcg
     3781 tggaggtggt cgaggcgatg atgctgtcgg gcgcaagcgg cgaaagccgc cccagcactt
     3841 gccgcttgag ttcgggcagc tccggcacgg cttcgaacac gacggcggct tgcgccatcg
     3901 gcgccgccgc ctcgcgctcc ggcagcactg agacccgcgc caggatgccg tcgaccgcgc
     3961 tcggcggcaa caggccaa
//
LOCUS       DASTMT010000277         3640 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_3684143, whole genome shotgun sequence.
ACCESSION   DASTMT010000277 DASTMT010000000
VERSION     DASTMT010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3640)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3640)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3640
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3684143"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..700)
                     /locus_tag="VFR00_06420"
     CDS             complement(<1..700)
                     /locus_tag="VFR00_06420"
                     /inference="COORDINATES: protein motif:HMM:NF013625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HEU0158925.1"
                     /translation="MTPRRARQALCVFVLLALAVAYNALFRQLRPVVGGGGIAAELPA
                     AAAPAPTLPNKAANAGAERTAAPKDAPDKRAQRAGRAAKPEAANGEATGSALGSQKSE
                     TARPETVRAIGRELRQRGYGSAAGEGAPRAQRSAAIMAYEYDNGLPLTGQASEALLER
                     ILFGGPGAGEGQGKVASAEAAELVRFVQQALSALGYQPGPVNGQLQADTVRAIREFEL
                     DKGLVPRGRISPELM"
     gene            953..1336
                     /locus_tag="VFR00_06425"
     CDS             953..1336
                     /locus_tag="VFR00_06425"
                     /inference="COORDINATES: protein motif:HMM:NF036461.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5330 domain-containing protein"
                     /protein_id="HEU0158926.1"
                     /translation="MFLFRIVFWLGLAVLLLPTEEHQQAKLYETATASVERIATFCER
                     NPKTCAAGAEFWATFLKKAEFASRVAIDLISSRGQRSEATAPNLTPRPTGAAAEPRPA
                     SPPRNTLTREDLGPAWRAPPPRAGA"
     gene            1467..1892
                     /locus_tag="VFR00_06430"
     CDS             1467..1892
                     /locus_tag="VFR00_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufE family protein"
                     /protein_id="HEU0158927.1"
                     /translation="MSIDAIRADFELLDDWEDRYRYVIELGRALPPLPEALRTEANKV
                     RGCASQVWLASKVAPSAPGKPPALTFQGDSDAHIVRGLIAILFALYAGRSAAEILRTD
                     ARALFGDLGLGEHLTQQRSNGFFSMVERIRADAEALQSA"
     gene            complement(1941..2348)
                     /locus_tag="VFR00_06435"
     CDS             complement(1941..2348)
                     /locus_tag="VFR00_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucR family transcriptional regulator"
                     /protein_id="HEU0158928.1"
                     /translation="MDEANRTDLVELTAEIISAYVSNNTVVAGDLPGIIQDVHEALSK
                     ASQRPGPVEREEPRPAIAVKKSVTPDYIICLEDGKKFKSLKRHLRTHYNLSPEEYREK
                     WALPHDYPMVAPNYAAARSQLAKQMGLGTRRDK"
     gene            2773..3204
                     /gene="msrB"
                     /locus_tag="VFR00_06440"
     CDS             2773..3204
                     /gene="msrB"
                     /locus_tag="VFR00_06440"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEU0158929.1"
                     /translation="MTKLTKPAADADKVVKTDAEWRSALSPEQYYVTRQHGTERAGSS
                     PLNKEKRQGTFTCVCCGAPLFSSAAKYESGSGWPSFDKPTDGDIVSEHADGSHFMQRT
                     EVRCARCEAHLGHVFPDGPPDTTGLRYCINGAALKFKPGES"
     gene            3298..>3640
                     /locus_tag="VFR00_06445"
     CDS             3298..>3640
                     /locus_tag="VFR00_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEU0158930.1"
                     /translation="MHGLKRPLKRLAPPIAERRPVFSVRHQVELVDEYAWLRADNWQE
                     VMREPALLAPEIRSYLEAENAFTQEALADTLPLQESLFAEMKARIKEDDSSVPAPDGP
                     FEYFTSYVTGGQ"
BASE COUNT          591 a         1249 c         1147 g          653 t
ORIGIN      
        1 ccatcagttc ggggctgatc cggcctctgg gcaccaggcc tttgtccagt tcgaattcgc
       61 gaatggcgcg cacggtatcg gcctggagtt ggccattgac cgggcccggc tgatagccaa
      121 gcgcgctcaa ggcctgctgc acgaagcgca ccagctcggc ggcctcggcg gaggcgacct
      181 tgccttgtcc ctcgccagca cccgggccac caaacagaat gcgctcgagc aaagcctcgc
      241 tcgcctggcc ggtcagcggc aagccgttat cgtactcgta ggccatgatc gccgcgctcc
      301 tttgcgcgcg cggcgcgccc tcgccggcgg cgctgccata gccgcgctgg cgcaattcgc
      361 gcccgatcgc acgcacggtc tcaggcctcg ccgtttcgct cttttggctg ccaagcgcgc
      421 tgccggtcgc ctccccattg gccgcctccg gcttggcagc gcggcctgcg cgctgagccc
      481 gcttgtcggg ggcgtccttg ggcgcggcgg tgcgctcggc gccggcattg gccgccttgt
      541 tggggagcgt tggggcgggc gcggcagcgg ccgggagctc ggcggcgatg ccgccgccgc
      601 ccaccacggg ccgcagctgg cgaaagaggg cattgtaggc gaccgccagc gccagcaaga
      661 caaacacgca cagtgcttgg cgtgcacgcc gcggcgtcat tgcgccctcc tcgatgcgaa
      721 gccttcccct gtttgcctcc accgcgcaat actcgggccc gattgctaac gaacgattaa
      781 cggcggcgcg caaagcgcgc cgccgcgccg gggccgatcg ctaaaattcg cgctaaattt
      841 gtcgccagtt gtgggctcgc gctttgcgct taagccacgc ttgtctgcta tggctcggga
      901 gacttgcgcc gaagaccggc gttggcgcac aaaacacgag gcggcaggcg tcatgttttt
      961 gttcaggatc gtgttctggc tcggtctcgc cgtgttgctg ctgccgaccg aggagcacca
     1021 gcaggccaag ctctacgaga cggccacggc cagcgtggag cgcatcgcca cgttctgcga
     1081 gcgcaacccg aagacctgcg cggcgggggc cgagttctgg gccacgttcc tcaaaaaggc
     1141 ggaatttgcc agccgcgtcg ccatcgatct catcagttcg cgcggccagc gcagcgaggc
     1201 aactgcgcca aacctgacgc cgcggccgac cggggctgcg gcggagccgc ggccggcatc
     1261 gcccccgcgc aacaccttga cgcgcgagga cctgggaccg gcctggcgcg ccccgccgcc
     1321 gcgcgctggc gcctgagccc ccggcgtcac cgcgtcagct tgcgtcgccg cggcttgaga
     1381 ccatatgcat agggcaacga cggccaaggc ctgctgcggt gatcggtgcc gcaagcgcag
     1441 gcgttagggt tttttgcacg tccgccatgt cgattgatgc cattcgtgcg gatttcgagc
     1501 tcctcgacga ttgggaggac cgctaccgct acgtcattga gctcggccga gccctgccgc
     1561 cgctgcccga ggcgctgcgg acggaggcca acaaggtgcg cggctgcgcc agccaggttt
     1621 ggctggcaag caaggtcgcg ccaagcgccc ccggcaaacc gcccgccctc acctttcagg
     1681 gcgacagcga tgcccacatc gtgcgcggct tgatagcgat tttgtttgcc ctctatgcgg
     1741 ggcgctcggc cgcggagatc ctgcgtacgg atgcgcgcgc gctgttcggc gacttgggcc
     1801 tcggcgagca cctgacccag caacgctcca atggcttctt ttccatggtg gagcgcattc
     1861 gcgccgatgc tgaggcgctg caatcggcct gacgcttggg cgttggcggt gagcaagccc
     1921 cggccgcggc gcgttttggg ctatttgtcg cgtcgcgtgc cgagccccat ctgcttggcg
     1981 agctgcgagc gggcggcggc gtagttgggg gccaccatcg ggtagtcgtg cggcaaggcc
     2041 catttctcgc ggtattcctc gggcgatagg ttgtagtggg tacgtaagtg ccgcttcagg
     2101 gatttgaatt tcttgccgtc ttccaggcag atgatgtagt ccggcgttac cgatttcttg
     2161 accgcgatgg ccggccgcgg ctcctcccgc tcgaccgggc cgggccgctg cgaggccttg
     2221 ctgagggcct cgtgcacatc ttggattatc cctggtaggt ccccagcaac aaccgtgttg
     2281 ttgcttacat aagctgagat gatctcggcg gtgagctcca caaggtcggt tctattcgcc
     2341 tcgtccatct gatcctctcc gttgctcgcg cgaaaatcgc ggcctcgaat cctcgaacct
     2401 tgatcccagc cgttaccgtg caaactacgt atacccattt ttacattatg gaccggagca
     2461 ttacaatgct aatgctgcaa cataaagaaa taaggcgaga ctgtgcaagc aatcctcgcc
     2521 ttgatctaag cctttgcgag cttatatata gcgcacagct ttacaccaat gttgcaagtg
     2581 catgacgttt tggacgtatg gcttgcccaa tactccacct gacaggcgga gctggggctg
     2641 ctacaacgct cctgccccga cacgaagact tgaaaacgct actctcgaac cgggcgcccc
     2701 cggccgtcat aatgggggat ccgccggttc acgcagtgga tcgtcgccaa caaacgccag
     2761 gccccgcccc ccatgaccaa gctgaccaag cccgcagccg acgccgacaa agtcgtcaag
     2821 accgacgctg aatggcgcag cgctttgagc ccggaacagt actacgtcac gcgccagcac
     2881 ggcaccgagc gcgccggctc ctcgcccctc aacaaggaga agcgtcaggg caccttcacg
     2941 tgtgtgtgct gtggcgcgcc cttgttttcc tccgcggcca aatacgagtc gggctcgggc
     3001 tggcccagct tcgacaagcc gaccgatggc gacatcgtca gcgaacacgc cgacggctcc
     3061 cactttatgc aacgcaccga ggttcgctgc gcgcgctgcg aagctcacct cggtcacgtc
     3121 tttcccgatg gaccgccgga taccacgggt ctgcgctact gcatcaacgg cgccgcactc
     3181 aagttcaagc ccggggaaag ctgacgccgc tcaccgccct tgtggcgggg ctgcctcccc
     3241 ctgcggggac gtcggcccgc ccgcctcgtc tgcccgcgcc ctctgaacag gtaccccatg
     3301 cacggcctca agcgacccct caagcgcctc gccccgccca tcgccgagcg ccgacccgtc
     3361 ttctcggtgc gccatcaggt cgagctggtc gacgagtacg cctggctgcg tgccgataac
     3421 tggcaggagg tcatgcgcga gcccgcgttg ctggcgcccg agatccgctc ctacctcgag
     3481 gccgagaacg ccttcacgca ggaggcgctg gcggacacgc tgcccttgca ggagagcctg
     3541 tttgccgaga tgaaggctcg catcaaggag gatgacagct cggtcccggc gcccgacggc
     3601 cccttcgaat atttcaccag ctacgtcacg ggcgggcagt
//
LOCUS       DASTMT010000278         2053 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_4084542, whole genome shotgun sequence.
ACCESSION   DASTMT010000278 DASTMT010000000
VERSION     DASTMT010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2053
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4084542"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..197)
                     /locus_tag="VFR00_06450"
     CDS             complement(<1..197)
                     /locus_tag="VFR00_06450"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU0158931.1"
                     /translation="MWRSRDFIVPAPVRKIALVSACVRGWRVLMAARTRVAVIGAGIV
                     GVTTASFLLRKGHDVVLLDPG"
     gene            373..1143
                     /locus_tag="VFR00_06455"
     CDS             373..1143
                     /locus_tag="VFR00_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018454014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HEU0158932.1"
                     /translation="MHNDFGGPRLARGGKAVYGARLGILMLEARFPRIPGDMGHAGTW
                     PFPVLYKVVKGASPQRVVAEQATGLLPSFIAAAKELVALGADGITTNCGFLSLYQKEL
                     AAAVTVPVATSSLMQLPMIESTLPPGKRVGVITVSAANLTHKHLLAIGLDPTLPVVGT
                     DQGREFTRVLIGNEETLDVAAAEQDILEAGDALLRQDARVGAVLLECTNMAPYARALR
                     DRLGLPVYDIYSFMLWFHAGLAPRDFGHPGSAARPWHE"
     gene            complement(1162..1773)
                     /gene="phnN"
                     /locus_tag="VFR00_06460"
     CDS             complement(1162..1773)
                     /gene="phnN"
                     /locus_tag="VFR00_06460"
                     /inference="COORDINATES: protein motif:HMM:TIGR02322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism
                     protein/1,5-bisphosphokinase (PRPP-forming) PhnN"
                     /protein_id="HEU0158933.1"
                     /translation="MADDGSSRDGEVCGLGPGRVVLLVGPSGAGKDAVLWQARGRLAG
                     RKQFAFVRRVVTREPDGAEDHDCVSASEFSARLRQGRFALNWQAHGLNYGIPVEIDAA
                     VRGGGCVVLNASRTIIAGARARYRNVAVVLIDAPAELRADRLAKRRREHGCEVAARLA
                     RATGFQPTDADLTIDNAGALSAAAKTFVDWLLTGEAAQAVSDD"
BASE COUNT          358 a          705 c          647 g          343 t
ORIGIN      
        1 tcgcccggat ccagaagcac gacatcgtga cccttgcgca acaaaaagct ggccgtcgtg
       61 accccgacga tgccggcgcc gattacggca accctggttc gcgccgccat aagcaccctc
      121 cagcctcgaa cacaagctga cacaagggca attttacgga ccggcgctgg cacgataaag
      181 tcgcgcgaac gccacatgcg gcgcctgttt ttgcgttgcc atgagcagga ggagaaaccg
      241 tgcgcacgac gtcgccagga gggccgtgcg gggctttcta cctctatcgg gtgcgcctcc
      301 gcctgggggg ccgccgatgc cgcgagccgg gcgccttttt gttgtagacc tggatcactc
      361 gggagcgccc tgatgcacaa cgattttggc ggcccgcggc tcgcccgtgg cggcaaggcc
      421 gtctacggcg cgcgcctcgg cattctgatg ctcgaagcgc gctttccgcg cattccgggc
      481 gacatgggcc atgccggcac ttggccgttt ccggtgctat acaaggtggt gaaaggagcc
      541 tccccgcagc gtgtcgtggc cgagcaggcg acagggctcc tgcccagctt catcgcggcg
      601 gccaaggagt tggtggcgct cggcgccgac ggcatcacca ccaattgcgg cttcctctcg
      661 ctttatcaga aagagctggc agccgccgtg acggtgccgg tggcaacctc gagcctgatg
      721 cagctgccca tgatcgagag cacgttgccc cccggcaaac gggttggcgt cattacggtc
      781 agtgctgcca acctgacaca caagcacctc ctggctattg ggctcgaccc gaccctgccc
      841 gtggtcggca ccgatcaagg tcgtgaattc acgcgtgtgc tgatcggcaa cgaagaaacg
      901 ctcgatgtgg ccgccgccga gcaggatatc cttgaggccg gcgacgcgct gttgcgccaa
      961 gacgcgcggg tgggcgccgt tctgctcgag tgtaccaata tggcgcccta cgcgcgtgcc
     1021 ctgcgcgacc gcttgggcct gccagtctac gatatctata gtttcatgct ttggttccat
     1081 gccggattgg caccgcgcga cttcggccat cccgggtcgg ccgctcgccc ctggcacgag
     1141 taagccagtg agcaccgcgg ctcaatcgtc gctcacggct tgcgcggctt cgccggttaa
     1201 cagccaatcg acgaaggttt tggccgccgc cgacaaggcg ccagcgttgt cgatagtcag
     1261 gtccgcatcg gttggctgga agcccgttgc ccgcgccagg cgcgcggcga cctcgcaacc
     1321 gtgctcccgc cggcgctttg ccagccggtc ggcacggagc tcggcgggag catcgatcag
     1381 caccactgcg acgttgcgat accgtgcgcg cgcgccggca atgatcgtgc gcgatgcatt
     1441 caacacgacg caaccgccgc cgcgtaccgc cgcgtcgatc tcgaccggga ttccatagtt
     1501 gagaccgtgt gcttgccaat tgagggcaaa gcggccttgc ctcagccgag ccgagaattc
     1561 gctggcactg acgcagtcat gatcttcggc gccgtcgggc tctcgggtca cgacccgcct
     1621 gacgaaggca aattgcttgc gaccggccaa gcggccacgg gcttgccaca gcaccgcatc
     1681 cttgccagcg ccgctgggtc ccacgagcaa gacgacccgg cccggcccga gcccgcaaac
     1741 ctcgccgtcc ctagatgacc cgtcgtccgc cacgatagac ctcaagtaca acgggaagct
     1801 cgcccagcat ccggatgcgc gccgatccgt acgcaacccg gcggcaatct ggccacgatc
     1861 gttcaagccg gcggcccgcg ccgggtgcag ggtaaccgtg cgaagggcgt ccggcaaacc
     1921 atggctctca attaagagaa cgtgccagat cgaatgcccc catcagcggg cttgccggca
     1981 cgtagtcgga cgcgaagatg tcgagcacgc cttcgcgcgc cagcgcttcg gccgccacat
     2041 tgccggagcg cga
//
LOCUS       DASTMT010000279         4796 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_147673, whole genome shotgun sequence.
ACCESSION   DASTMT010000279 DASTMT010000000
VERSION     DASTMT010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4796)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4796)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4796
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147673"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..342)
                     /locus_tag="VFR00_06465"
     CDS             complement(<1..342)
                     /locus_tag="VFR00_06465"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEU0158934.1"
                     /translation="MAVAIGGAAAAKTKPVEMLIDGNWVASTSGDWIGVESPGNRRLF
                     ASIPRGNAEDVDRAVKAASAAFAGWSKVVPRERGRILQKIADAVDARVEELARIIAEE
                     TGNALRTQARPE"
     gene            complement(451..3810)
                     /locus_tag="VFR00_06470"
     CDS             complement(451..3810)
                     /locus_tag="VFR00_06470"
                     /inference="COORDINATES: protein motif:HMM:NF019344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU0158935.1"
                     /translation="MGTGVGIIIGLIGGLLIALVLNRRQAAMPAAPASTPGPQAPPNP
                     DAAPTEPTTLTARLHRLEGTFASYSANYAHPRELEDQAGFHEAAKLLADPAVSLEVVS
                     QYALGSNWALSCAALAALSARTDGSELAQVISADFDKLHLWAMFFALRYLGALEHRRP
                     VGEPLAAAKDWWAENVLIIGFFRDYFQQRSADGDSASFAGSLQRTPSASTAAIRGLLE
                     RINHPMARELIVELDSLQRTNVDRGFLASFGRFWSDSKDEDFLIELDEWREELEVAQA
                     TAQLNPVRSLLVSGEARVGKTSFLHLLARRLARRGWTIFEAGGADLMAGQQWFGQLEG
                     RIQRTIEEAAVSKKLIWYIPDILQLARSGTHQGQSASILDQILPAIQSGRLLVWIEVS
                     PKSLARLLQFRPALRGLMEVVRLEPMDQDRTAALAQGLIARLGPKMRLTVEPGTIDAA
                     LSSAHQYLSSGHLPGSAIDLIRLTLNRASKTGGSKLAAGDVLATLAQLTGLPISILDN
                     RERVDLGEIRGYFCARVIGQNEAVGAIVERIAMLKAGLNDPGKPIGVFLFAGSTGTGK
                     TELAKTAAEYLFGSTDRLIRLDMSEFQSAESTSKIIGDGGVGGETDSLIARVRKQPFS
                     VVLLDEFEKAHPNIWDLFLQVFDDGRLTDSMGHVADFRHCMIVLTSNLGATSHDSRGL
                     GFAPAATSFTEEQVLRAIGQTFRPEFQNRLDKVIVFRPLTRDLMREILKKELNGVLDR
                     RGLKDREWAVEWEASAQDFLLEKGFSAAMGARPLKRAIDQYVIAPLAATIVERRFPEG
                     DQFVFVKSDGRAIQAEFVDPDADAGADIAELEEGAPGLLSIVLKPTGSAAELATLSAE
                     LQTIEQSLASAEWETLRLGLAQEMSGADFWKHPGRHATLARFALLDRVGAAMKTAGAL
                     EARLMRGRERRGNASRELAGRLALQLHLIKEGIKDALEAAPIEVAVRVEPVFEKPAAP
                     HDTRQWCERLLAMYRGWANNRHMQLSELGGDEMRHACLLISGFGAHRLLAQEVGLHVF
                     EGEEDKSPRRATARVSLAVAPLGDVPIEKQRRLLTQAFEAGPRSGSIVRRYRSTPSPL
                     VRNMNGSWRSGRLEEVLRGDFDLIAASQSVP"
     gene            complement(3923..4387)
                     /locus_tag="VFR00_06475"
     CDS             complement(3923..4387)
                     /locus_tag="VFR00_06475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0158936.1"
                     /translation="MSLSHARQHASFGLTATSRQARRLARLTVGLMLAGGLAAASVPV
                     SAAEAAGLEGTWSGGGTVRFASGAEEHARCRAHYSRRSQVVYVLRATCATASGKAAQS
                     ATLQKVGDNRYSGTFYNSEFDISGRIYVILRGSSQSVRLTSGMGSASFRLSR"
BASE COUNT          798 a         1613 c         1591 g          794 t
ORIGIN      
        1 ctcggggcgc gcctgggttc tcagcgcgtt gccggtctcc tcggcaatga tcctcgccaa
       61 ttcctcgacg cgggcgtcga ccgcatcggc gatcttctgc agaatgcgtc cgcgctcgcg
      121 ggggaccacc ttgctccagc cggcaaaggc ggccgaggcg gccttgacgg cgcggtccac
      181 gtcttcggca ttgccgcgcg ggatgctggc gaataggcga cgattgcccg ggctctccac
      241 gccgatccag tcgccggagg tgctggccac ccaattgccg tcgatcagca tctccacggg
      301 cttggtctta gccgccgccg ccccgccgat cgctactgcc atggcgattt tctccgcaaa
      361 agtggccctt gcgggcaagc atatcgcgcg gcttcgggag ggtgaaggag agtttcgccc
      421 gccctcgcga gcgcggcctt gccggcgagg tcacggcacc gattggctgg cggcaatcag
      481 atcgaaatcg ccgcgcaaaa cctcctcgag ccggccgctg cgccaacttc cgttcatgtt
      541 gcgcaccagg ggcgagggtg tgctgcggta gcggcgcacg atgctgccac tgcgagggcc
      601 ggcctcgaag gcttgcgtga gcagacggcg ctgcttttcg atcggcacgt cgcccagcgg
      661 cgccacggca aggctgaccc gggcggtggc gcgccggggg ctcttgtcct cttcgccctc
      721 gaacacgtgc aggcccacct cctgcgccaa caggcggtga gcgccgaagc cgctgatcag
      781 caggcaggca tggcgcatct catcgccgcc gagttcgctc agctgcatgt ggcggttgtt
      841 ggcccaaccg cgatacatgg cgagcagccg ttcgcaccat tgccgcgtat cgtgaggcgc
      901 ggccggcttt tcgaacacgg gctcgacccg cacggccacc tcgatcggag cggcctcgag
      961 cgcatccttg ataccttcct tgatcaggtg cagctgcagc gccagccggc cggccagctc
     1021 gcgcgaggcg ttgccgcggc gctcgcgccc gcgcatgaga cgcgcctcga gcgcgcccgc
     1081 cgtcttcatc gcggccccga cgcggtccag cagggcgaaa cgcgccagcg ttgcgtgccg
     1141 cccgggatgc ttccagaaat cggcaccgga catctcctgc gccaggccga ggcgcagggt
     1201 ctcccactcc gccgaagcca gcgactgctc gatggtttgc aactcggcgc tcagcgtggc
     1261 gagttcggcc gcacttcccg tcggcttgag gacgatggag agcaagcccg gcgcgccttc
     1321 ctcgagctcg gcgatgtcgg caccggcatc ggcgtcggga tcgacgaact cggcctggat
     1381 ggcgcgcccg tcactcttca cgaacacgaa ctggtcgcct tcgggaaagc gccgctcgac
     1441 gatggtggcc gccagcggcg cgatcacgta ctggtcgatc gcgcgcttga gcgggcgggc
     1501 acccatggcg gcggaaaaac ccttctcgag caggaagtcc tgcgccgagg cctcccattc
     1561 gaccgcccat tcccggtctt tcaggccacg gcggtcgagc acgccattga gctccttctt
     1621 caggatctcg cgcatcaggt cgcgcgtcaa cggacggaag acgatgacct tgtcgagccg
     1681 gttctggaat tctggccgga aagtctggcc gatggcgcgc aacacctgct cctcggtgaa
     1741 gctggttgcc gccggggcga agccgagacc cctactgtcg tggctggtgg cgccgagatt
     1801 ggaggtgaga acgatcatgc aatggcgaaa gtcggcgaca tggcccatgg agtccgtcaa
     1861 ccggccatcg tcgaacacct gcaagaacag gtcccaaata ttgggatgcg ccttctcgaa
     1921 ctcatcgagc agaaccacgg aaaagggctg cttgcgtacg cgggcgatga gcgaatccgt
     1981 ctcgccgccg acaccgccgt cgccgatgat cttgctggtc gactccgccg actggaactc
     2041 gctcatatcg agccggatca gccgatcggt ggagccgaac aagtattccg ccgccgtttt
     2101 ggcgagctcg gtcttgccgg tgccggtcga acccgcgaac aggaatacgc cgatcggctt
     2161 gcccggatcg ttcaagcccg ccttgagcat ggcgatgcgc tcgacgatgg cgccgacggc
     2221 ctcgttctgg ccgatcacgc gggcgcagaa atagccgcgg atctcgccca gatcgacgcg
     2281 ctcacgattg tcgaggatgc tgatcggcaa gcccgtcaac tgcgcgagcg tggcgagcac
     2341 gtcgccggcc gccagcttgc tgccgccggt tttgctcgcc cggttgagcg tgaggcggat
     2401 cagatcgatg gcggagccgg gaagatggcc cgaactcagg tactgatgcg ccgagctcaa
     2461 ggccgcatcg atggtcccgg gctcgaccgt gagccgcatc ttgggcccta gccgagcaat
     2521 caggccttgc gccagggcgg cggtgcggtc ttggtccatc ggctccagac gcacgacttc
     2581 catcagcccg cgtagcgccg ggcggaattg caagagccgc gccaggctct tgggcgagac
     2641 ctcgatccac accaacaacc ggccggattg aatggcgggt aggatctgat cgagaatgct
     2701 cgctgactgg ccttgatgcg tgccgctgcg cgccagttgt agaatatcgg ggatgtacca
     2761 gatcagcttc ttgctgaccg cggcttcctc gatggtgcgc tgaatgcggc cctcgagctg
     2821 gccgaaccac tgctgccccg ccatcagatc ggcgccgcca gcttcgaaaa tcgtccagcc
     2881 gcgccgcgcc agccggcgcg cgagcaggtg caagaaggag gtcttgccca cccgcgcctc
     2941 gccgctgacc agcaaagagc gcactgggtt cagctgggcg gtggcctgcg ccacctcgag
     3001 ctcctcgcgc cactcgtcga gctcgatcag gaagtcttcg tccttgctgt cggaccaaaa
     3061 gcggccgaag gaggcgagaa agccccggtc gacgttggtg cgttgcaggc tgtcgagctc
     3121 gacgatgagc tcgcgcgcca tcggatggtt gatgcgctcc agcaagccgc ggatggctgc
     3181 ggtcgacgcc gacggcgtcc tctgcaggga gccggcgaag ctggcgctat cgccgtcggc
     3241 gctccgctgc tggaagtagt cgcggaagaa gccaatgatg agcacgttct cggcccacca
     3301 atccttggcg gcggccaacg gctcgccgac gggccgtcta tgctcgagcg ccccgagata
     3361 gcgcagcgca aagaacattg cccacaaatg cagtttgtcg aagtcggcgc taatgacctg
     3421 cgccagctcg gaaccgtcgg tgcgcgcact cagggctgcc aaggcggcgc aggagagcgc
     3481 ccagttgctg cccagcgcat actggctcac gacttcgagc gacaccgcgg gatcggccaa
     3541 cagcttggca gcctcatgga agcccgcttg gtcctcgagc tcgcgcgggt gggcataatt
     3601 ggccgagtag gaggcaaaag ttccctccaa gcgatgcagc cgcgccgtca aggtggtggg
     3661 ctcggtcggg gcggcgtccg gatttggcgg cgcctgaggc ccaggcgtgc tcgccggtgc
     3721 ggccggcatg gcggcctggc ggcgattgag caccagggcg atcagcaggc ccccgatcag
     3781 cccgatgatg atgccaacgc ccgtacccaa ccccgtcccc cagcttttgt gctctgccga
     3841 gccggggcag gaaaggcgct ccgagaggcc ctgtcaacgt tcgccggtgg gcgggccgcc
     3901 gcgtcggcgg cagcccttgg cgctagcggc tgagccggaa cgaggccgat cccatgccgc
     3961 tggtgagccg aacgctctgg ctgctgccgc gcaagatcac gtaaatgcgc ccgctaatgt
     4021 cgaattcgct attgtaaaaa gtgccggaat agcggttgtc gccgaccttt tgcagcgttg
     4081 cgctctgcgc cgccttgccc gacgccgtgg cgcaggtggc gcgcaggacg taaacgactt
     4141 gcgaacggcg gctgtaatgg gccctgcagc gggcgtgctc ttcggcgccg gaggcaaacc
     4201 gcacggtgcc gccgcccgac caggttcctt ccaatcccgc cgcctccgcc gccgacaccg
     4261 gcacggatgc ggccgccagt cccccggcca acatcaggcc aacggtcagc cgcgccagtc
     4321 tcctcgcctg ccttgacgtc gcggtcagac cgaagctcgc gtgctggcgc gcatgggaaa
     4381 gagacatcgg cgttgatctc ctggcgacgc accgaccatc tctgattcgg cgctcaaggt
     4441 gtatgcgggg gtgcccgcgc aaacaggtat attcgcttgc cttaactcca cctgaatacg
     4501 caacttgcag gttggttccc gcggttgctc gtcgacatgg cgatgcggcg accgaagcgc
     4561 aagaaggcgg cagcgcgcgc tgccttcacg aaaagcggcg cttgcgcttg tcgcgcagcg
     4621 tcgttggccg cgccggcgcc gggaatgggc gcacgccggt gcgccgcggc gattggcgcc
     4681 gcagtctatt tcgggcagat gtaacccgtg ccgctcggat ttttaaagca gcccaccgac
     4741 tcgggcaaaa acaactttgg cagatagagc accacgtccg gcgccatgac cgtaaa
//
LOCUS       DASTMT010000280         1697 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_2443384, whole genome shotgun sequence.
ACCESSION   DASTMT010000280 DASTMT010000000
VERSION     DASTMT010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 1697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1697)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1697
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2443384"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..135
                     /locus_tag="VFR00_06480"
     CDS             <1..135
                     /locus_tag="VFR00_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007615123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase"
                     /protein_id="HEU0158937.1"
                     /translation="LIPIVENAGGIITTWDGEPARQGGRVIAAGDARTHRLALRLLAG
                     "
     gene            complement(160..1119)
                     /locus_tag="VFR00_06485"
     CDS             complement(160..1119)
                     /locus_tag="VFR00_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU0158938.1"
                     /translation="MDLVALAKNPVPSGATTGTFKGAGGLNIRFARWAPTRGPRRGTV
                     CLFHGRGEYIEKYFEVIADLRRRGFCVATMDWRGQGGSDRLLRNRRKGHVRSFVDYDR
                     DLARFMQEIVLPDCPAPFIALAHSMGGNILLRNARLPGTWFQRMILSAPMIAIAREQL
                     GVPQGAARAYTEILAALGLSSLYVRHGGDAVLEATPFEQNPVTSDRERYLRSRMVLES
                     APALGLGSPTIGWLRAALRSIAKIRAPDYPLRVELPILLFAAGADSIVSTQAVEEFGL
                     RLKVGTHVLIPQAKHEILQEADTIRGHFWAVFDEYLGVTTAVA"
     gene            complement(1293..>1697)
                     /locus_tag="VFR00_06490"
     CDS             complement(1293..>1697)
                     /locus_tag="VFR00_06490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0158939.1"
                     /translation="DIYRRPERPFAGTPPQPYAGPPLPRERVYRDDEEAPPYGRPPRY
                     SYGDVGPGYGGQCVPRDVIRDRLTRHGWHDFHDGALRGNVAVVRARRPSGRLFELTIE
                     RCSGEIVNAYPLEGRVFGPFAYGPRPWWQRPY"
BASE COUNT          307 a          543 c          577 g          270 t
ORIGIN      
        1 ctgatcccca tcgtcgaaaa tgccggcggc attatcacga cctgggacgg cgagcccgcg
       61 cgccaaggtg gacgcgtcat tgccgccggg gatgcgcgca cgcaccgtct ggccttgcgc
      121 cttctcgccg gctgaggcgg cgcccggccg ggagcgttgt caagcgacag ccgtggtgac
      181 gccgaggtat tcgtcaaaca ccgcccagaa atgccccctg atggtatcgg cttcctgcaa
      241 gatttcgtgc ttggcctgcg gaattaaaac gtgggtgccg accttgagac gaaggccaaa
      301 ctcctcgacg gcctgcgtcg agacaatgct gtcggcgccg gccgcgaaca gcaggatggg
      361 gagctcgaca cgcagggggt agtccggcgc tctgatcttg gcgatggagc gtagcgcggc
      421 cctgagccaa ccgatggtgg gcgatccaag tcccagcgcg ggtgcgctct ccagcaccat
      481 gcggctgcgc aaatagcgct cccgatccga ggttactggg ttttgctcga agggcgtcgc
      541 ctcgagcacc gcatcgccac cgtggcgcac gtacagcgag ctcaagccca gggcggccag
      601 gatctccgta taggcgcggg ccgcaccctg ggggacgccg agctgctcgc gcgcgattgc
      661 gatcatgggt gcgctcagga tcatgcgctg aaaccaggtg cccggcaggc gcgcgttgcg
      721 caaaaggatg ttgccaccca tcgagtgcgc gagcgcaatg aagggcgccg ggcaatcggg
      781 cagcacgatc tcctgcatga agcgggcaag gtcgcgatca tagtcgacga acgaacgtac
      841 gtgccctttg cggcggttgc ggaggagccg atccgagccg ccttggcctc gccagtccat
      901 ggtggcgacg caaaaaccgc gccggcgcag atcggcgatc acttcaaagt atttctcaat
      961 gtactcgccc cgaccatgga ataagcacac tgtgccgcgc ctggggccgc gggtcggagc
     1021 ccagcgcgca aagcgaatgt tgaggccacc ggcacctttg aaggttcctg tcgtggcgcc
     1081 gctcgggact gggttctttg caagtgccac cagatccatg caacgtctcc gccagctgct
     1141 ttgggcgggc tctatagcag gcgggtcgcc gcgccaacag atgcggcatg agccgtgcgg
     1201 gccgttgcca aagcaaaaga ccccagcact tgctggggtc ttgctctcgt atcggcgcgc
     1261 acgcttgcag gtgcccgaag cttgggcgct tatcaataag ggcgttgcca ccaaggtcgc
     1321 gggccgtaag cgaacggacc aaagacgcgc ccttcgagcg gataggcgtt gacgatctcg
     1381 ccggagcagc gctcgatcgt cagttcgaac aaacgtccac tgggccggcg ggcgcgcacc
     1441 acggcaacgt tgccccgcag cgcgccgtcg tggaaatcat gccatccgtg ccgcgtcaag
     1501 cggtcgcgaa tgacgtcgcg cggcacgcac tggccgccgt agccggggcc tacgtcgcca
     1561 taagagtagc ggggcggccg cccgtacggg ggcgcctctt catcatcgcg ataaacccgc
     1621 tcgcgcggga gcggagggcc tgcgtagggc tgcggcggcg tgccagcaaa ggggcgctcg
     1681 ggccgacgat aaatgtc
//
LOCUS       DASTMT010000281         2558 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_3104023, whole genome shotgun sequence.
ACCESSION   DASTMT010000281 DASTMT010000000
VERSION     DASTMT010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2558
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3104023"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..573)
                     /gene="pyrH"
                     /locus_tag="VFR00_06495"
     CDS             complement(<1..573)
                     /gene="pyrH"
                     /locus_tag="VFR00_06495"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEU0158940.1"
                     /translation="MAAAASSALRYRRVLLKLSGEALMGTGAYGIDMRVVQRLALDIK
                     EALAAGAEIAAVVGGGNIFRGLAGVAKGMDRATGDYMGMLATVMNALALQNGLEQAGV
                     QARVLSAIPMPTICEAFVRSKALHHLENGRVVIFAAGTGNPYFTTDTTAALRAVEMNC
                     EVLVKATQVDGVYSADPKQDPKAVRHDRLTY"
     gene            complement(834..1772)
                     /gene="tsf"
                     /locus_tag="VFR00_06500"
     CDS             complement(834..1772)
                     /gene="tsf"
                     /locus_tag="VFR00_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013948487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HEU0158941.1"
                     /translation="MTAISAGQVKQLRDKTGAGMMDCKTALTETRGDMEQAVDWLRKK
                     GLSKAATKAGRVAAEGLVGVAVSDKTGAIVEVNSETDFVARNDKFQDMVRKVGTVAAK
                     ANGDLAKLLAMPFPGSGGSVEDHVKDMVATIGENMSVRRTATLSVAEGAVADYVHNKA
                     ADGLGKIGVLVALESKGEPKAVAALGRQLAMHIAAASPAAVTAEELDPALIARERAIY
                     SEQASASGKSADIVAKMVEGRLRKEFYQQVVLLQQTFLGAGGDGKQTVEQVLQDAAKK
                     LNAPVKIAKFVRYALGEGIDKKDDDFAAEVAKAAGR"
     gene            complement(1852..>2558)
                     /gene="rpsB"
                     /locus_tag="VFR00_06505"
     CDS             complement(1852..>2558)
                     /gene="rpsB"
                     /locus_tag="VFR00_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012334544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEU0158942.1"
                     /translation="GGTLTNWKTISQSIRRLKLLDEMLVGDVKGRTKKEVLQLTRDRD
                     SLNRSLGGIKEMGGLPDLLFVIDTNKEQIAIAEAKKLGIPIVAIVDTNCDPDGIDFVV
                     PGNDDAGRAITLYCDLIARAAIDGIERGQTQAGVDVGAAEAPPAEDLAGDKQPPFKGI
                     EAPRGEADDLKRLPNVNPRLEQRLNDAGVYHFWQIADLDEQATAGLDRLLNLKGRIER
                     DGWVAAAKKLVEGQAA"
BASE COUNT          405 a          883 c          794 g          476 t
ORIGIN      
        1 ataggtcagc cgatcgtggc gcaccgcctt ggggtcctgc ttggggtcgg ccgagtagac
       61 gccatcgacc tgggtggctt tcacgagtac ttcgcagttc atttccacgg ccctcagcgc
      121 cgccgtggtg tcggtggtga agtaggggtt gccggtgccg gcggcgaaaa tgacgacacg
      181 gccattttcc aagtgatgca aggccttgga gcgcacgaac gcctcgcaga tggtgggcat
      241 gggaatggcg gataaaaccc gcgcctgcac gccggcctgc tcaagcccgt tttgcagcgc
      301 cagggcgttc atcactgtgg ccagcatacc catatagtcg ccggtggccc gatccatgcc
      361 cttggccacg ccggcaaggc cgcggaaaat gttgccgccc cccaccacgg ctgcgatctc
      421 cgcccctgcc gccagcgcct ccttgatgtc gagggccaag cgctgcacga cccgcatgtc
      481 gatgccgtag gcgcccgttc ccatcaaggc ctcgcccgaa agcttcagca acacgcgtcg
      541 gtagcgtagg gcagaggacg cggcggctgc catcacacca gttccctcaa cgatcggagc
      601 cacaactcag acacagccgg ttcagttcga gccggcacgg cgattaggcc cgcgaggccc
      661 gctcgcgcgc acagccagtg ggccgccaac cccgcgcacc ctggcgccct ggtcgctgat
      721 gcgggcggcc ctttgagcgc cttgccgtca agacataacc ccacgcccgg tcggtgcgca
      781 aatcggggcc cggcgcgccg cagcgctgag cttgaaccaa gctgcccctt ggcctagcgg
      841 ccggcggcct tggccacctc ggcggcgaaa tcgtcatcct tcttgtcgat gccctcaccc
      901 agcgcatagc gcacgaactt ggcgatcttg acgggcgcgt tcagcttttt ggcggcgtcc
      961 tgcagcacct gctcgacggt ctgtttgcca tcgccacccg caccgaggaa ggtttgctgc
     1021 aagagcacga cctgctggta gaactctttg cgcaaccggc cctccaccat cttggcgacg
     1081 atatcggccg acttgccgct ggcgcttgcc tgctcgctgt aaatggcgcg ctcgcgggcg
     1141 atcaaggccg gatcgagctc ctcggcggtc accgccgccg ggctggcggc ggcaatgtgc
     1201 atggccaatt gtcggccaag cgcggcgacc gccttgggct cgcccttgct ctcgagcgcc
     1261 accagcacgc cgatcttgcc gaggccgtcg gcggccttgt tgtggacgta gtccgccacc
     1321 gccccttccg ccaccgataa ggtggcggtg cggcgcaccg acatgttctc gccgatggtg
     1381 gccaccatgt ccttgacgtg gtcctcaaca ctgccgccgg agccgggaaa cggcatggcc
     1441 aggagcttgg ccagatcgcc gttggccttt gccgccacgg tgccgacctt gcgcaccatg
     1501 tcctggaact tgtcgttgcg ggcaacgaaa tccgtctcgg agttcacctc gacgatggcg
     1561 cccgtcttgt cgctgacggc gaccccgacc aacccctcgg cggcgacgcg accggccttg
     1621 gtcgcggcct tggagagacc ctttttgcgc agccaatcga cggcctgctc catgtcgcct
     1681 cgcgtctccg tcagggcggt cttgcagtcc atcatgccgg cgccggtctt atcgcgcagt
     1741 tgcttgacct gacctgcaga aatagctgtc atgtgttcct cgctttagcc cagccgggcg
     1801 ccgtcggcgc ggcgccgggg gtcaataaac ttaaaagctc gggctgccgc ctcaggcggc
     1861 ctgcccttcg accaatttct tggcggcggc gacccaaccg tcgcgctcga tccgaccctt
     1921 gaggttgagc agacggtcga gcccggccgt cgcttgctcg tcaagatcgg cgatctgcca
     1981 gaagtggtag acaccggcat cattgagccg ctgctccagt cgcgggttga cgttgggcag
     2041 ccgcttgaga tcgtcggcct cgccacgcgg cgcctcgata cccttaaatg gtggctgttt
     2101 gtcgccagcc aaatcctcag ccgggggcgc ttcggcggcg ccgacatcga cgccggcctg
     2161 ggtctggccg cgctcgatgc cgtcgatggc tgcgcgcgcg atcagatcac agtaaagcgt
     2221 gatcgcgcgc ccggcgtcgt cgttgcccgg aaccacgaaa tcgatgccat ccggatcgca
     2281 gttggtatcg acgatggcca cgatcggaat gccgagcttc ttggcttcgg cgatggcaat
     2341 ctgctccttg ttggtgtcga tcacgaacaa caaatccggc agcccaccca tctccttgat
     2401 gccgccgagc gagcgattga gcgagtcgcg gtcgcgcgtc agctgcagaa cttccttttt
     2461 ggtgcgaccc ttgacgtcgc ctaccagcat ctcgtccagc agcttcaagc gccggatcga
     2521 ctgcgagatc gtcttccaat tggtcagcgt gccgccga
//
LOCUS       DASTMT010000282         9867 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_5655570, whole genome shotgun sequence.
ACCESSION   DASTMT010000282 DASTMT010000000
VERSION     DASTMT010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 9867)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9867)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9867
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5655570"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(157..1170)
                     /locus_tag="VFR00_06510"
     CDS             complement(157..1170)
                     /locus_tag="VFR00_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012497189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HEU0158943.1"
                     /translation="MHERLNLITDLPGVSVGHAEDTTVASGVTAILLDRDNVASGTIR
                     GGAPGGRDTALLEPEMTVEGLDAVVLAGGSLFGLDAAGGVVSFLREADKGLKIGAATV
                     PIVAQAIIFDLLNGGNKAWGRMPPYWELGWQAAQRAAPGTFALGSVGGGLGATTANLK
                     GGLGSASTTTASGFAVGAIVVVNAVGSVTVGDGPHFWAAAYERDGEFGGLGAPPTPAA
                     GALDLRIKGGAPPATSIAFVATDAIMTKAQAKRLALMADDGLARAIRPAHAPMDGDTV
                     IAAAMLRKPMLEGPLNLTEIGLAAGDCIARAVARAVYLASALPFAGAQPAWRDRFAGG
                     RGP"
     gene            complement(1319..2437)
                     /locus_tag="VFR00_06515"
     CDS             complement(1319..2437)
                     /locus_tag="VFR00_06515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015916932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HEU0158944.1"
                     /translation="MKKVLWPALAIAAAALVGAGGASAQLKVGVAGPLTGPNAAFGAQ
                     LKNGAEQAAADLNEKGGINGQKIELVFGDDASDPKQGVSVANKMAGDGVKFVIGHFNS
                     GVTMPASEVYQENGIYMITPSATNPRITERNLWNVFRTCGRDDQQGEVAGKYILSKFK
                     GKKVAIVHDKTTYGQGLADETKKAMTKGGMKEVLYEGINIGEKDFSALVSKIKASGAD
                     VVYWGGLHTEGGLLVRQMRDQGVKAPLMGGDGITSDEFASIGGPGVEGTLMTFGPDPR
                     KNPAAAEAVKKFAAKNINPEAYTLYSYAALQVIAMAAADAKSLDPKKVAEATKAGKAF
                     KTVIGDLAYNKKGDITRLDYVMYVWKKNPEGKITYFQE"
     gene            complement(2578..2943)
                     /locus_tag="VFR00_06520"
     CDS             complement(2578..2943)
                     /locus_tag="VFR00_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009494116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0158945.1"
                     /translation="MTSAFGAYLYEGGDNVLWIFLLLTVLLGGAAAYASGKAIAQTWR
                     PFRQVPLYALLLAAGVRFCHFALFEEPLLSAPSYAVDFLVALLFAALGFRLLRARQMA
                     TQYSWLFQRSGLFGWRRAG"
     gene            complement(2936..3661)
                     /locus_tag="VFR00_06525"
     CDS             complement(2936..3661)
                     /locus_tag="VFR00_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0158946.1"
                     /translation="MSEPLLSVRDVKSFYGNIMALKGVAIDVAEGEIVTLIGANGAGK
                     STLMMTIFGNPRAREGRIYYQGRDITDLPTHAIARLRIAQSPEGRRIFPRMTVRENLQ
                     MGAEIDEGRHFEEDLERVCRLFPVLARRLEQRGGTLSGGEQQMLAIARALMSRPKLLL
                     LDEPSLGLAPLIVKQIFAVIGELNRQGMTVFLVEQNAFHALRLAHRGYVMVSGLITMS
                     GTGKELLARPEIRAAYLEGGRHD"
     gene            complement(3658..4569)
                     /locus_tag="VFR00_06530"
     CDS             complement(3658..4569)
                     /locus_tag="VFR00_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015916935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0158947.1"
                     /translation="MRAAQAPLLTVEHLTMRFGGLLAIDDLSFSAARGEITAIIGPNG
                     AGKTTVFNCITGFYKPSGGRLLLNLSEETPAADQIDQLTASGSRAAAIPGGELFLLER
                     MPDYEIAARAKMARTFQNIRLFAGMTVLENLIVAQHNRLMAASGWTVLGLLGAPQYRE
                     AEASALDKAKYWLDKVGLTERADDPAGDLPYGAQRRLEIARAMCTEPTLLCLDEPAAG
                     LNPRESADLKRFLKSLQVEHATSLLLIEHDMSVVMEISDHIVVLEYGQKISDGPPDHV
                     RNDPKVIAAYLGVEDEEVEEAEAEITQ"
     gene            complement(4569..5945)
                     /gene="livM"
                     /locus_tag="VFR00_06535"
     CDS             complement(4569..5945)
                     /gene="livM"
                     /locus_tag="VFR00_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007562556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivM"
                     /protein_id="HEU0158948.1"
                     /translation="MASVRADAPRSPWRSLANAVTDALGAALLALGLAVPILALRTEQ
                     NISNELILQPRWGYVAIAVAGTFAARLGYLLFSSLKRRPRRLKPAVPSSWRETAARSL
                     AVVGLGLLALYPLLALALTGKAGAIKWIDNFGIQIMIYIMLGWGLNIVVGLAGLLDLG
                     YVAFYAVGAYSSALLAQNFGFSFWICLPLAGILAAFWGILLGFPVLRLRGDYLAIVTL
                     AFGEIIRLILINWVPVTGGYAGISGIPRPTLLGLPFNASEEGFAAFFNLEFSPVHRAI
                     FLFYVILALALLTNWVTLRLRRLPIGRAWEALREDEIACRSLGINTTTTKLTAFAIGA
                     GFGGFAGAFFAARQGFISPESFTFMESAIVLAIVVLGGMGSQIGVAIAAVAMIGGTEI
                     LRELDWLRRIFGPDFEPTQYRMLLFGLAMVLVMIWKPRGLIAFRMPSIFLSERRRVRA
                     DLVQEGHG"
     gene            complement(5951..6868)
                     /locus_tag="VFR00_06540"
     CDS             complement(5951..6868)
                     /locus_tag="VFR00_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012319217.1"
                     /note="LivHMGF is the membrane component of the LIV-I/LS
                     branched-chain amino acid transporter; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease LivH"
                     /protein_id="HEU0158949.1"
                     /translation="MEYLLQQLINGVTLGSIYGLIAIGYTMVFGIIGMVNFAHGDVFM
                     LSTFIALIFLLALTQTLGFGSLALALLIVLVLGMLLTSLWAWAIERLAYRPLRGSFRL
                     APLISAIGMSIFLSNLVQVTQGPRNKPVPSFLETVLTLREGENYVVTLSYKQVLIVGV
                     TVLLLGAFWYLVQNTALGRAQRACEQDRRMASLLGIDVDRTISLTFVIGAALAAVAGL
                     LFMIYYGVVSFSDGFVPGVKAFTAAVLGGIGSLPGAVLGGLLIGLIETLWSAYFSIDY
                     KDVATFSILAITLVFLPSGLLGKPEVEKV"
     gene            complement(7137..7379)
                     /locus_tag="VFR00_06545"
     CDS             complement(7137..7379)
                     /locus_tag="VFR00_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013947083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043231 - intracellular membrane-bounded
                     organelle [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HEU0158950.1"
                     /translation="MSDEPICAQKGPYQVDLVEGRAYFWCTCGRSTKQPFCDGSHKVT
                     SLEPLRFIATASGTVNLCGCKGTDDRPYCDGAHNML"
     gene            complement(7502..7822)
                     /gene="soxZ"
                     /locus_tag="VFR00_06550"
     CDS             complement(7502..7822)
                     /gene="soxZ"
                     /locus_tag="VFR00_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate oxidation carrier complex protein
                     SoxZ"
                     /protein_id="HEU0158951.1"
                     /translation="MNKPRVKLPDAAKAGDLIEIKTLISHVMETGQRKDPDGKVIPRN
                     IINSFSATFAGNEVFKADLQPGISANPYLSFFMRVPGPGEFEFTWVEDGGNKLVEKIK
                     LNVT"
     gene            complement(7842..8315)
                     /gene="soxY"
                     /locus_tag="VFR00_06555"
     CDS             complement(7842..8315)
                     /gene="soxY"
                     /locus_tag="VFR00_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011443242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate oxidation carrier protein SoxY"
                     /protein_id="HEU0158952.1"
                     /translation="MTEVHPTPRLDRRQFATGAAGLVLFALSTGRLGAQVPEPAWQQL
                     LKNLVGDSKPIEGKLTLELPEIAENGNTVPLTVSVESPMTANDYVKAIHVIATENPQP
                     GVATFRFSPLSGKASAATRMRLARTQEVIGLAELSDGKFLLGRRTVKVTIGGCGG"
     gene            8797..>9867
                     /locus_tag="VFR00_06560"
     CDS             8797..>9867
                     /locus_tag="VFR00_06560"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HEU0158953.1"
                     /translation="MISRRGFARLLGQAGAGALSIGCLSPFALAQRAPRVVIVGGGAG
                     GATVAHYLKMNAAELDVTLIEARQIYSSSFFSNLYLGGLRPLDSFNHSYVALQRLGVK
                     VVHDFATDVDAARKTVKTRGGRTYGYERLVLSPGIAVQYEAIPGYSHEVARALPHAYT
                     TNPAGTRNLKRQLLAMRDGGTVLIAAPNDPYRCPPAPYERACMIAHYLKAKKPKAKLV
                     LLDPKKAFANQALFLEAFDRHYKGIIEVNLSTEIDDFALISVDPKGKEVSTRAGKRLK
                     FDVANIIPPQRAGDIAVRAGCTQGNWCPIDPSSFASRNVQDVYVLGDAAIAAEMPKSA
                     FAANSQAKVVAQDILVHLAGKAA"
BASE COUNT         1722 a         3224 c         3274 g         1647 t
ORIGIN      
        1 ggatcggcct cggcaccagc ggcccgcacg ccgtgccggt ccacgcccca ccgctgtcga
       61 gacaccccct gaccagctgc tcgcggtgtg cctgccagcc gaacagaaga aggaggacgc
      121 tgacgagcgc gagggcgacg atcaggcgcc gtgggctcat ggtccgcgtc cccccgcgaa
      181 ccggtcgcgc caggccggct gcgccccggc aaacggaagc gcgctcgcca ggtagaccgc
      241 acgggcgacg gcgcgcgcga tgcagtcccc ggccgcgagg ccgatttcgg tcaaattgag
      301 cgggccctcg agcatgggtt tgcgcagcat ggcggcggcg atcacggtgt ccccgtccat
      361 cggcgcgtgc gccgggcgga tggcgcgggc gaggccgtca tcggccatca aggcgaggcg
      421 cttggcctga gccttggtca tgatcgcatc ggtggcgacg aaggcgatgc tggtggcggg
      481 cggggctccg cctttgatcc tgaggtcgag ggcgcccgcg gcgggcgtgg gcggggcgcc
      541 gaggccgccg aactcgccgt cgcgctcgta ggctgccgcc cagaaatgcg ggccatcgcc
      601 caccgtcacg ctgccgaccg cgttgacgac gacgatggcc ccgaccgcga agccggaggc
      661 cgtcgtcgta ctggccgagc caagcccgcc tttgaggttg gccgtggtgg cgccgagccc
      721 gccaccgacg ctgccgagcg caaaggtccc cggcgccgcg cgctgcgccg cttgccagcc
      781 gagctcccag tagggtggca tgcgtcccca ggccttgttc ccgccgttga ggagatcgaa
      841 gatgatggcc tgtgccacga tcggcaccgt ggccgcgcca atcttgagac ccttgtcggc
      901 ctcgcgcaaa aacgacacca cgccgccggc cgcgtcgagc ccgaacagcg agccgcccgc
      961 aagcacgacg gcgtcgagac cctcaaccgt catttccggt tcgagcagcg cggtgtcgcg
     1021 accgccgggc gcgccgccgc gaatggtccc ggaggcaacg ttgtcgcggt cgagcaggat
     1081 ggccgtgacg ccgctggcga cggtggtgtc ctcggcgtga ccgacgctca cgccgggcag
     1141 atcggtaatg aggttcaagc gctcgtgcac gaagtgggct ccggctgtgc tcgacgtgag
     1201 gagcggcggc caggctcggc gccgccggcc tggggcgcct gcgccaaggc cacgctccga
     1261 aacgcgaaga ggcagccctg tggggctgcc tcctcattgg ctcttggctc agcgcccctt
     1321 actcctggaa gtaggtgatc ttgccttccg ggttcttttt ccacacgtac atgacgtagt
     1381 cgagccgcgt gatgtcgcct tttttgttgt aggcgaggtc gccgatcacc gtcttgaagg
     1441 ctttgccggc cttggtcgct tccgcgacct tcttggggtc gagcgacttg gcgtcggccg
     1501 cggccatggc gatgacctgc agcgcggcgt aactgtagag cgtgtaggct tcggggttga
     1561 tgttcttggc ggcgaacttc ttgaccgctt cggccgccgc cgggttcttg cgcggatcgg
     1621 ggccgaaagt catcagcgtg ccctcgacgc ccggcccgcc gatcgaggcg aactcgtccg
     1681 aggtgatgcc atcgccaccc atcaggggtg ccttgacgcc ctggtcgcgc atttgacgta
     1741 cgaggagccc gccctcggtg tgcaagccgc cccagtagac gacatcggca ccggaggcct
     1801 tgatcttgga gaccagggcg gagaagtcct tctcgccgat gttgatgccc tcatagagca
     1861 cctccttcat gccgcccttg gtcatggcct ttttggtctc gtcggcaagg ccctgcccgt
     1921 aggtggtctt gtcgtgaacg atggcgacct tcttgccctt gaacttggac agaatgtact
     1981 tgcccgccac ttcgccctgc tggtcgtcgc ggccgcaggt gcggaacacg ttccacagat
     2041 tgcgctcggt gatgcgggga ttggtggccg acggcgtgat catatagatg ccgttttcct
     2101 gatagacctc ggaagccggc atggtcaccc cggaattgaa gtgaccgatc acgaacttga
     2161 cgccgtcgcc ggccatcttg ttggccaccg agacgccctg cttgggatcg gacgcgtcgt
     2221 cgccgaacac aagttcgatt ttctgtccgt tgatgccgcc tttttcgttg aggtcggccg
     2281 cggcctgttc ggccccgttc ttcaattgcg ccccgaacgc cgcgttcggg cccgtcaggg
     2341 gaccggccac gccgaccttg agctgggcag acgcgccgcc ggcgccgacg agcgctgcgg
     2401 cggcaatggc cagggctggc caaagcacct ttttcatgaa agcctccgtt tgatccgaca
     2461 ctgacaggct ccggcggtgc gagcccgcct cggcgtgcat gattcaacga aagcagagcc
     2521 gagtcacgcc gatttgccgg gccttatggc gccgatctgc gcccgaataa tggctgccta
     2581 gccggcccgc cgccagccga atagcccgct gcgctggaac agccagctgt actgcgttgc
     2641 catctggcgc gcgcgtagga ggcggaagcc cagcgcggcg aacaagagag cgacaagaaa
     2701 atcaacggcg tagctgggcg ccgaaaggag cggctcctcg aatagggcaa aatggcaaaa
     2761 gcgcacgccg gcggccagca acagggcata gagcggcacc tggcggaacg ggcgccacgt
     2821 ctgcgcaatg gccttgcccg aggcgtaggc agccgccccg ccgagcagca ccgtcagcag
     2881 caaaaagatc cacagcacat tgtcgccgcc ttcgtagagg taggcgccga acgcactagt
     2941 catggcgccc gccttccaga taggcggccc ggatctcggg acgcgccaac agctccttgc
     3001 cggtgccgct catggtgatg agcccgctca ccatcacgta gccgcgatgc gcgagcctca
     3061 gcgcgtggaa ggcgttctgc tcgaccagga aaacggtcat gccctgccga ttgagctcgc
     3121 cgatcaccgc gaagatctgt ttgacgatca ggggcgcaag cccgagcgag ggctcatcga
     3181 gcagcagcag cttgggccgg ctcatcaggg cgcgcgcgat cgccagcatc tgttgctcgc
     3241 cgccggacag cgtccccccg cgctgctcga ggcggcgggc cagcaccgga aacaggcggc
     3301 agacgcgctc caggtcctcc tcgaagtggc gaccctcgtc gatctcggct cccatctgca
     3361 ggttctcgcg cacggtcatg cgcgggaaaa tccgccgccc ttccggtgac tgggcaatgc
     3421 gcaggcgggc gatggcgtgc gtcggcagat cggtaatgtc gcggccctgg tagtagatgc
     3481 ggccctcgcg ggcgcgcggg ttaccgaaga tcgtcatcat caaagtcgac ttgccggcgc
     3541 cgttggcccc gatcagcgtg acaatctcgc cttctgcaac atcgatcgcg acgcccttca
     3601 gcgccatgat gttgccgtag aagcttttca cgtcccgcac cgacaagagc ggctcgctca
     3661 ttgcgtgatc tccgcctccg cttcctccac ctcctcgtcc tccacgccga gataggcagc
     3721 gatcaccttc gggtcgttgc gcacatgatc gggcggtcca tcggaaattt tctgtccgta
     3781 ttcgagcacc acgatgtggt cggaaatctc catcaccacc gacatgtcat gttcgatgag
     3841 gagcaatgag gtggcatgct cgacctgcag cgacttcaga aaacgtttga ggtcggcgga
     3901 ttcgcgtggg ttgaggccgg ccgcaggttc gtcgagacac aacagcgtgg gctcggtgca
     3961 catggcgcgg gcaatttcga gccggcgctg cgcgccgtag ggcagatccc cggccgggtc
     4021 gtcggcccgc tcggtcagcc caaccttgtc caaccagtac ttggctttat ccagcgcgct
     4081 cgcctcggcc tcgcggtact gcggcgcgcc gagcagaccc agcaccgtcc agccggaggc
     4141 ggccattaag cggttgtgct gggccacgat caagttttcc agcacggtca tgcccgcaaa
     4201 caagcgaatg ttctggaagg tacgggccat cttggcgcgc gcggcgatct cgtagtcggg
     4261 catgcgctcg agcaggaata gctcgccgcc gggaattgcc gcggcccgtg agccggacgc
     4321 ggtcagctga tcgatctgat cggccgcggg cgtctcctcg ctcaagttca agagcagccg
     4381 gccgcccgag ggtttgtaga agccggtgat acagttgaag accgtggtct tgcccgcgcc
     4441 gttcgggccg atgatggccg tgatctcccc gcgcgcggcc gagaacgaca aatcgtcgat
     4501 ggcgagaagg cctccgaacc gcatggtcag gtgctcgacg gtgagcagcg gtgcctgcgc
     4561 ggcgcgcatc agccgtgccc ctcttgcacg aggtcggcgc gcacccggcg gcgctcgctg
     4621 agaaagatcg agggcatccg gaaggcgatc agccctctgg gtttccagat catcaccagc
     4681 accatggcca atccgaacag cagcatgcgg tattgcgtgg gctcgaagtc gggtccgaag
     4741 atgcggcgca gccaatcgag ttcgcgcagg atctcggtgc caccgatcat ggccacggcg
     4801 gcaatggcga caccgatttg gctgcccatg ccgcccagca cgacgatggc cagcacgatt
     4861 gcactttcca tgaaggtgaa ggattcaggg ctgatgaagc cctggcgcgc ggcgaagaac
     4921 gccccggcga aaccgccgaa gccggcgccg atggcaaagg cggtgagctt ggtcgtggtg
     4981 gtgttgatgc cgagcgagcg gcaggcgatc tcgtcctcgc gcaaggcctc ccaggcgcgg
     5041 ccgatcggca ggcggcgcag gcgcagcgtg acccaattgg tcaacagcgc cagcgccagg
     5101 atcacgtaga acagaaagat cgcgcggtgc accggcgaga actcgaggtt gaagaaggcg
     5161 gcaaagccct cctcgctggc gttgaagggc agccccagca gcgtcggccg cggaatgccg
     5221 gaaataccgg cgtagccacc ggtcaccggc acccagttga tcaggatcag gcggatgatt
     5281 tcgccgaagg ccaaggtgac gatggcgagg tagtcgccgc gcaggcgcag caccggaaag
     5341 ccgagcagaa tgccccagaa cgccgccagg atgccggcga gcggcaggca gatccaaaag
     5401 ctaaagccga agttttgcgc cagcagcgcg ctggagtagg cgcccacggc atagaaggcc
     5461 acataaccga gatcgagcag accggcgaga cccaccacga tgttcaagcc ccatcccagc
     5521 atgatgtaga tcatgatctg gatgccgaaa ttgtcgatcc acttgatcgc tccggccttg
     5581 ccggtgagcg ccagcgcgag caagggatag agcgcgagca ggccgagacc gacgacggct
     5641 aagctgcgcg cggcggtctc gcgccagctg ctgggaacgg cgggcttgag gcgccggggc
     5701 cggcgcttga gggagctgaa cagcaggtag cccagccggg cggcgaaggt gcccgccacg
     5761 gcgatggcca cgtatcccca gcgcggctgc aggatcagtt cgttggagat gttctgctcg
     5821 gtgcgcaaag cgaggatcgg cacggccaga cccagcgcca ggagcgcggc gccgagtgca
     5881 tcggtgacgg cgttggcgag cgaccgccag gggctccgag gggcatcggc acgcacgctc
     5941 gccatcgccg tcacaccttc tcgacctcgg gtttgcccaa caggccggag ggcagaaaca
     6001 ccagcgtgat ggcaaggatg gagaaggtgg caacatcttt gtagtcgatg gaaaagtagg
     6061 ccgaccacaa tgtttcgatg aggccgatca acaaaccgcc caaaacggcg ccgggcagtg
     6121 agccgatccc gcccagcacc gcggcggtga aggccttaac gcccggcacg aagccgtcgg
     6181 aaaagctgac gacgccgtag tagatcatga agagcaggcc ggcgacggcg gccagcgcgg
     6241 cgccgatcac gaaggtgagc gaaatggttc tgtcgacgtc gatgccgagc agcgaggcca
     6301 tgcgccgatc ctgttcgcag gcgcgctgag cgcggccgag cgccgtgttc tgcacgagat
     6361 accagaacgc tcccagcagc aggacggtga ccccgacgat cagcacctgc ttgtaggaga
     6421 gggtgacaac gtaattttcc ccctcccgca aagtgagaac cgtttccagg aaagacggca
     6481 ccggcttgtt gcgcgggccc tgcgtcacct gcacaagatt ggacaggaag atcgacatgc
     6541 cgatggcgga aatcaacggt gccagccgga aggagccgcg caaggggcgg taggccaaac
     6601 gttcgatggc ccatgcccac aacgacgtta aaagcatgcc gagcacgaga acgatcaaca
     6661 acgccagggc gagcgagcca aagcccaggg tttgcgtcag cgccaacaga aagatcagcg
     6721 caatgaaggt cgacagcatg aaaacgtcgc cgtgcgcaaa gttcaccatg ccgatgatgc
     6781 cgaacaccat ggtgtagccg atggcgatga gcccatagat ggagcccagc gtgacgccgt
     6841 taatgagctg ctgcaggagg tattccatgc ggggttcgcc tgcctctgcg cgccggtgcg
     6901 cgctttgtcg agtcgctgca gcaggctagc agaccccttc gcgccgcgtc acctcgctct
     6961 cggccgcgaa cacgctttgg ggacgggttg gctcccttcg accacaaagg gccgcaaggc
     7021 gccccggggt tgcgcccgtg ggggtggggt gccgccggtc gcccgcaacc cgccccggcg
     7081 ggggtgccgc tgggccggcc ttgtgaggct ccggccagcc cttaaagccc ccagccctag
     7141 agcatattgt gggcgccgtc gcagtagggc cggtcatcgg ttcccttgca gccgcacagg
     7201 ttcaccgtgc ccgaggcggt ggcaatgaac cgtagcggct cgaggctggt gaccttgtgg
     7261 gagccatcgc agaagggctg cttggtggag cgtccgcagg tgcaccagaa gtaggccctg
     7321 ccctcgacca ggtccacctg gtaggggcct ttttgcgcac agatcggctc gtcggacatg
     7381 cttgacctcg gttagctcgc ctcggttggc tcgcctcggt tggctcgcct cggttggctc
     7441 tggggcgcca gcgcctgggc ggcgtcggcc tcgccgtgcc gtcccggcgg cctcttcccc
     7501 ctcaagttac gttgagcttg atcttttcga ccagcttgtt gccgccgtcc tcgacccagg
     7561 tgaattcgaa ctcgcccggc cccggcaccc gcatgaaaaa ggacagatag gggttggctg
     7621 aaatgccggg ctgcaggtcg gccttgaaga cctcgttgcc cgcgaaggtc gccgagaagg
     7681 aattgatgat gttgcgcggg atcaccttgc cgtccgggtc cttgcgctgg cccgtctcca
     7741 tgacatggga gatcaaggtt ttgatctcga tcaggtcgcc cgctttggca gcatcgggca
     7801 gcttgacgcg tggcttgttc atgtcccgct ccttcgcgcg gctagccgcc gcagcctccg
     7861 atcgtgacct tcacggtgcg ccggccgagc aggaacttgc cgtcgctgag ctcggcgagc
     7921 ccgatcacct cctgcgtgcg cgcaaggcgc atgcgggttg ccgccgaggc cttgcccgag
     7981 agcgggctga accggaaggt ggcaacgccg ggctgcggat tttcggtggc gatcacgtgg
     8041 attgccttga cgtagtcgtt ggcggtcatc gggctctcga cggagaccgt gagcggtacg
     8101 gtgttgccgt tctcggcgat ttccggcagc tccaaggtca gcttgccctc gatcggtttg
     8161 ctgtcgccga ccagattttt caagagctgt tgccaggcgg gctccggcac ctgggcgccg
     8221 agccgacctg tcgacagcgc aaagaggacg agccccgccg cgccggtcgc gaattgccgg
     8281 cgatcgagcc gcggtgtcgg gtgtacctct gtcatcctca agccatccta gatcctgcac
     8341 atatgccgcg gctcgacacg tccctggacg cctgccggcc gccgtcgagg tgcgaaacat
     8401 aagccctgcg ccggctttgc caaggggtcg gcgcgaccct cttgcgcagt atgccgtgaa
     8461 ccgaccgccc aatccacctc tgggccacct gtgggccacc tctggggccg ccgatgccgc
     8521 gcccgaggtg tgctcaaggc gacgggcgtt tacctcgccg cccgcgcgcc tttgcgaacc
     8581 cgcattcttc agccatactc gcgaaggcgg acctccccaa gcgttttttg ccgtttgcgg
     8641 cggggagctg gcccggcgct cgcggttttg cgccacgggg cgcgcgcgca tcctggcgat
     8701 ggactttgaa cggtccgctg cctgaagctg gcggaagcga caatcctggt gcgccggatc
     8761 tcaggtccga ccgcgtcgtc ggggcaaggt gggcagatga tttcaagacg gggctttgcg
     8821 cggctgctgg ggcaggcggg cgccggcgcg ctctccatcg gttgcctcag cccgttcgcc
     8881 ctcgcccagc gcgccccgcg cgtcgtgatt gtcggcggcg gagccggcgg ggcgacggtg
     8941 gcgcattatt tgaagatgaa cgccgccgag ctcgacgtta cgttgatcga agcccggcag
     9001 atctacagct cctcgttttt ttccaacctt tacctcggcg gcctgcgccc cctcgactcc
     9061 ttcaatcata gctacgtggc cttgcagcgg cttggcgtca aagtcgtgca cgattttgcc
     9121 accgacgtcg acgccgcgcg caagaccgta aagacgcggg gcggccgcac atatggctat
     9181 gaacgcttgg tgctatcgcc cggcatcgcc gttcagtacg aggcgatccc cggttattcg
     9241 cacgaggtgg cgcgcgccct gccgcacgcc tacaccacca atccggccgg cacgcgcaat
     9301 ctgaagcgcc aattgctggc gatgcgcgac ggcggaacgg ttttgattgc cgcacccaac
     9361 gacccctacc gctgtccgcc ggcaccctac gagcgtgcct gcatgatcgc ccactacctc
     9421 aaggccaaga agcccaaggc gaagctcgtc ctgctcgacc ccaaaaaggc ttttgccaat
     9481 caggcgctat tcctggaggc cttcgaccgc cactacaaag ggattatcga ggtcaacctc
     9541 agtaccgaaa tcgatgattt cgcgctgatc agcgtggacc ccaagggcaa ggaagtctcc
     9601 accagagccg gcaaacgcct caagttcgac gtcgccaaca tcattccccc gcagcgcgcc
     9661 ggcgacattg cggtgagggc cggctgcacg cagggcaact ggtgccccat cgatcccagc
     9721 agtttcgctt cgagaaacgt gcaagatgtc tacgtgctgg gcgatgcggc gatcgcagcc
     9781 gagatgccga agtcggcatt cgccgccaac agccaggcga aagttgtggc ccaggatatc
     9841 ctcgtgcacc ttgcgggcaa ggcggcg
//
LOCUS       DASTMT010000283         2104 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_3105215, whole genome shotgun sequence.
ACCESSION   DASTMT010000283 DASTMT010000000
VERSION     DASTMT010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2104)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2104)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2104
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3105215"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..919)
                     /locus_tag="VFR00_06565"
     CDS             complement(<1..919)
                     /locus_tag="VFR00_06565"
                     /inference="COORDINATES: protein motif:HMM:NF040269.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD regulator"
                     /protein_id="HEU0158954.1"
                     /translation="MSYASLAPAAGTVAVGLTAVVLAVREDEPVVAVVGADGATTGPR
                     ATLPGGLFCAGEHDSLDASLRAAVARETGLELGQTRQIAAASTRAAGADAPITSVVLC
                     ISYLALVGPQAANQRASLAWTSWYHYFPWEDWRHGRPACLKQEIEPALNAWAAAEPDC
                     ASEHLGRRQRVRLCFGTDGGAWDEEKVVERYDLLCEAGVIGEDARDGKQRPSGAQLAA
                     LRHAELGDHGRALASAIAELRRMIKYRPVVFELMPEVFTLFELQKTVEAILGPHLHKQ
                     NFRRLVEGGGLVEPTDQYRFRTGGRPARLY"
     gene            complement(1054..1614)
                     /locus_tag="VFR00_06570"
     CDS             complement(1054..1614)
                     /locus_tag="VFR00_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012042569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein tyrosine phosphatase"
                     /protein_id="HEU0158955.1"
                     /translation="MSEREAKAAGQPGVLHVCPFSALPSLVARAKASHVITLLQEEIL
                     VERPSSILPERHLRLRVHDISEPLAGCVHPDPTHIEEIIAFARVWAGQGPMVVHCWAG
                     ISRSTAAAFTALCSINPDVPEDLIALHLRRASPTAYPNRLMIRLADQALGRSGRMVRA
                     VEAIGRGVIAAEAVPFAIAADLSRAS"
     gene            complement(1645..>2104)
                     /locus_tag="VFR00_06575"
     CDS             complement(1645..>2104)
                     /locus_tag="VFR00_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEU0158956.1"
                     /translation="AFSVAQHALLVEDIAASMHPDLTRRWRLAALLHDAPEYVIGDLI
                     SPFKAAVGLDYRAFERQLLEAIHRRFGLPPALPERVANAIKAADRIAAYYEATALAGF
                     TLAEANRYFGVPRGVPDNLRQRLAALAAKPIASAQQEFLVRFQDLSQGEA"
BASE COUNT          361 a          696 c          721 g          326 t
ORIGIN      
        1 ggtagagccg tgccggccga ccgccggtgc gaaagcgata ctggtcggtg ggctcgacca
       61 ggccgccgcc ctcgaccagg cgacgaaaat tctgcttgtg caggtggggg ccgagaatgg
      121 cttccacggt cttctgcagc tcgaagaggg tgaacacctc cggcatcagc tcgaacacca
      181 cggggcggta cttgatcatg cggcgcagtt cggcgatagc gctggcgagc gcgcggccat
      241 gatcgcccag ctcggcatga cgcaaggcgg ccaattgcgc gccggagggc cgttgcttgc
      301 cgtcgcgcgc atcctcgccg atcacgcccg cctcgcacaa caggtcgtag cgctcgacca
      361 ccttttcctc atcccaggca ccgccgtccg tgccaaagca caggcgcacg cgttggcggc
      421 ggccgaggtg ttcgctggcg cagtcgggtt cggcggccgc ccaagcgttc agcgccggtt
      481 cgatctcctg cttgagacag gcgggccgac cgtgccgcca gtcctcccag ggaaagtaat
      541 gataccagct ggtccaggcg aggctggcac gctggttggc cgcttgcggc ccgaccagcg
      601 ccaggtagct gatgcacaaa accacggagg tgatcggcgc gtcggcgccg gcagcgcgcg
      661 tgcttgccgc cgcaatttgc cgggtttgcc cgagctcgag gccggtttcg cgcgccaccg
      721 ccgcgcgcag ggaggcgtcg agactgtcgt gttcgccggc gcaaaaaagg ccgccgggca
      781 acgttgcgcg cggccccgtc gtggcaccgt cggcacccac gacggccacc accggctcgt
      841 cctcgcgcac ggccagaaca acggcggtca gaccgaccgc gaccgtcccc gcagcgggtg
      901 cgaggctggc gtagctcatg cgtcttatga cgccttcgga atggcgctgg ggccaagtca
      961 tgcagggtgc ctcgcggcca gtgagcgact tggctctaga ttgcgatccc aatcgtggca
     1021 attttccgca ctagctgaag gcggtagcaa aattcagctg gcgcgcgaca aatccgcggc
     1081 aatggcgaag ggcaccgcct cggccgcgat caccccgcgc ccgatcgcct cgacggcgcg
     1141 caccatgcgc ccgctgcggc caagcgcttg atcggcgaga cggatcatca aacgattggg
     1201 ataggctgtg ggtgaggcgc ggcgcaggtg cagcgcgatc agatcctccg gcacgtccgg
     1261 gttgatcgag cacaaggccg tgaaagcggc cgcggtcgaa cgagagatgc ccgcccaaca
     1321 gtgcacgacc atgggcccct gtcccgccca gacgcgggca aaggcgataa tctcctcgat
     1381 gtgcgtggga tcggggtgaa cacaacccgc cagcggctcg ctgatgtcgt gcacgcgcag
     1441 acgcaggtgt cgttccggca gaatgctgct cggccgctcc accagaatct cttcctgcag
     1501 cagggtgatg acatgggagg ccttggcccg cgcaacgagg ctcggcaggg cactgaaggg
     1561 acatacgtgc agcacgccgg gttgacctgc cgccttggcc tcgcgctcac tcatcgtcgt
     1621 tcctcacccg atcgctgctg gaggctatgc ctccccctgt gacaaatcct gaaagcgcac
     1681 gagaaactcc tgttgcgccg aggcgatggg cttcgccgca agcgccgcca ggcgttggcg
     1741 caggttatcg ggcacgccgc gcggcacccc gaagtagcgg ttggcttcgg ccagcgtaaa
     1801 gcccgcgagc gccgtcgcct catagtaggc ggcgatgcgg tcggccgctt taatggcgtt
     1861 ggcgacccgt tccggcagcg cgggtggcag cccgaagcgg cgatgaatgg cttccaacag
     1921 ctggcgctcg aaggctctgt aatcgagacc gacggcggcc ttgaagggac tgatcaagtc
     1981 gccgatcacg tattcggggg catcgtgcag gagcgcggcc agccgccagc ggcgcgtcag
     2041 gtccgggtgc atactggcgg cgatgtcttc gaccaacagg gcatgctggg ctacggaaaa
     2101 ggcg
//
LOCUS       DASTMT010000284         2368 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_5001265, whole genome shotgun sequence.
ACCESSION   DASTMT010000284 DASTMT010000000
VERSION     DASTMT010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2368)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2368)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2368
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5001265"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(181..1104)
                     /locus_tag="VFR00_06580"
     CDS             complement(181..1104)
                     /locus_tag="VFR00_06580"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEU0158957.1"
                     /translation="MRQAERQRDRSGKLNVRALLLLMVVVLAWGSMWPVNKALLAYVS
                     PIWSVALRTYISMLTLFAISLCLTGLVLPARQDLPVLISMVVLHMVAFTILSQLALQW
                     VPAGRSAVLAYTSVLWAPIGAAVFLGETLSRRRILGLGLGILGLLAIFNPFTFHWGDP
                     NAVLGNALLLLAAMLWAASIVHNRGHLWRSTPFQLAPWQALLAALLLTLAAWSIEGPP
                     QVTWTWQALLLLLFAGIPGTAIAYWAAAVSSSELPAATTSLGLLATPAVSVAVALVLL
                     GEAPSLALLLALALLIGGIAVGATGPGRPGS"
     gene            1538..>2368
                     /gene="msrP"
                     /locus_tag="VFR00_06585"
     CDS             1538..>2368
                     /gene="msrP"
                     /locus_tag="VFR00_06585"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018267070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HEU0158958.1"
                     /translation="MLIKRAADIAPSEITPQAAYLRRREFLAGTAALGLGALLPAAGR
                     AAPLAASKSPLSTTAEPQTALKDIISYNNFYEFGVDKDDPVKHAHTLKTKPWKVKVDG
                     LVGKPADYDLDDLIKDIALEERIYRMRCVEGWSMVIPWVGVPLASLLKRFEPQGSAKY
                     VAFETLVRPAEMPAQRGVFQPLPWPYVDGLRLDEAMHPLTILAVGLYGETLPNQNGAP
                     IRLVVPWKYGFKSVKSIVRIRFVEKMPHTAWNDANASEYGFYSNVNPTVDHPRWSQAT
                     E"
BASE COUNT          425 a          866 c          744 g          333 t
ORIGIN      
        1 tttcgtgccg ccttcctcac catcgccgcc ttcgccgcgg tcggagcggt gctggcctgg
       61 tcgatccctt cccgccggct cacttgaaaa aaagcgcaac gagagcgggt ccttccccct
      121 tgggcgcgcc cctcttgcgc acccccgcgt gaagcgcgcg gagacacccg cgccccggct
      181 ctaactgccg gggcggcccg ggcctgtggc gccgacggca atgccgccga tcaacagcgc
      241 cagcgccagc aacagggcta ggctcggcgc ctccccgagc aagaccagcg cgacggcgac
      301 gctgacggcc ggtgtcgcga gcagacccag cgaggtcgtc gccgcgggca actccgagga
      361 gctcaccgcc gccgcccagt aggcgatcgc cgtgccggga atgccggcaa acaacagcaa
      421 aagcagcgcc tgccacgtcc aagtcacctg cggcgggccc tcgatgctcc aggccgccag
      481 cgtcaacagc agcgcggcca gcagcgcttg ccaaggcgcc agctgaaacg gcgtcgaacg
      541 ccagagatgg ccgcggttgt gcacaatgct cgccgcccac agcatggccg ccagcagcag
      601 caaggcattg ccgaggacgg cgttcggatc gccccagtga aaggtgaagg gattgaagat
      661 cgccagcagc cccaggatgc cgagccccag gcccaggatc cggcgccgcg acagcgtctc
      721 gcccaggaaa acggcggcgc cgatcggggc ccacagcacg ctggtatagg ccaagacggc
      781 ggaacgcccg gccggcaccc attgcagggc cagttgcgag agaatggtga aggccaccat
      841 gtgcagcacc accatactga tgagaaccgg cagatcctgg cgcgcgggca aaacgaggcc
      901 ggtcaggcaa agcgagatgg caaacagcgt cagcatggaa atgtaggtgc gcagcgccac
      961 cgaccagatc gggctcacgt aggccagcaa agccttgttg acgggccaca tgctgcccca
     1021 agccagcacg acgaccatca gcagcagcaa ggcgcgcacg ttgagcttgc ccgaccgatc
     1081 cctctgcctc tccgcctgcc tcatggagcg gcccgccctc cccgcgctcg gccttgcccg
     1141 cgccgccggg ccccaagatc ccccacgcgc gcggcgccgg caaactccgg catccccccg
     1201 gcctttgatg gacgccacgg caagatcacg tcaagctccg gctcgcgcag gctgccgccg
     1261 ggccctcggg gggccaagcc cgggcgcccc cgcgcccccg catttttgtg cacttttgcg
     1321 ctgcggcagg ctgggccgcg ccccggtcgc cgccagacca gcgcggtgcg ccgccggcgc
     1381 cagccgcgcg ccacctcttg cggttctccg cctttgctgc tgtcattgac gtcccctttc
     1441 acaccctgtc acaagcccgt caggcgcccg catttcccct tccgctggag gcagcgggcc
     1501 tttatctcag gcgcacattg gccaagtgga cctcttcatg ctgatcaaac gcgctgccga
     1561 catcgcccct tccgaaatca ccccgcaggc cgcctatttg cggcgccgcg agtttctcgc
     1621 cggcacggcg gcgctcgggc tgggggcgct gctgcccgcg gcgggccggg cggctcccct
     1681 ggcggcgagc aaaagtccgc tctcgacgac ggcggagccg cagaccgcgc tgaaagacat
     1741 catcagctac aacaacttct atgagtttgg cgtcgacaag gatgatccgg tcaagcacgc
     1801 ccacacgctg aagaccaagc cctggaaggt gaaagtcgat ggcctggtcg gcaagcccgc
     1861 cgactacgac ctcgacgacc tcatcaagga tatcgccctg gaggagcgca tctaccgcat
     1921 gcgctgcgtg gagggctggt cgatggtgat cccctgggtc ggggtgccgc tggcaagcct
     1981 cctcaaacgc ttcgaaccgc aaggcagcgc caagtacgtg gccttcgaga ccttggtgcg
     2041 gcccgccgag atgccggccc agcggggcgt attccagccg ctgccctggc cctacgtcga
     2101 tgggcttcgc ctcgacgaag ccatgcaccc gctgaccatt ctcgccgtgg gcctctacgg
     2161 tgaaacgctt cccaatcaga acggcgcgcc gatccggctg gtcgtgccct ggaagtacgg
     2221 attcaagagc gtgaaatcga tcgtccgcat ccgcttcgtc gagaagatgc cgcacaccgc
     2281 gtggaacgac gccaacgcga gcgagtacgg tttctactcg aacgtcaatc cgaccgtcga
     2341 tcatccgcgc tggagtcagg cgacggag
//
LOCUS       DASTMT010000285         2183 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_481561, whole genome shotgun sequence.
ACCESSION   DASTMT010000285 DASTMT010000000
VERSION     DASTMT010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2183
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_481561"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..982
                     /locus_tag="VFR00_06590"
     CDS             <1..982
                     /locus_tag="VFR00_06590"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU0158959.1"
                     /translation="IGWWSDVLADAVQRSGKLKIVSCYTRSAAKRQAFADKYACRSAA
                     SYEALLADPEIEAIINTTPNTVHRLTCLAAAAAGKHVFLDKPIANSIADGRAITDACR
                     QAGVVLALGYQRRRESHFRWIKQQIDAGLFGRLVNAEANISRDRLGKIDLSSWRYRAD
                     GMPGGVMLQIGIHYTDVLEYLLGPIKAVRGQLAQLVLPGDNPDVASLLLQHESGALST
                     LNASYASASEYYLMNIYGKDATAYYDLHNGLRLSRRGEREPRRVPGPTTDTLVEELEE
                     FAAAIRGGGQIEVGGEYGTRSLAVVRAGILSAQQGRQIEVADLLKSDAGV"
     gene            1012..1983
                     /locus_tag="VFR00_06595"
     CDS             1012..1983
                     /locus_tag="VFR00_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009494539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEU0158960.1"
                     /translation="MKPRRHVRLIALLLLAGSLLPAAAQDYPSRSITVIVPFPPGGAS
                     DVVARIVTDQMSRLLKQAIVIENVGGAGGTLGSARAAAAAPDGYTLLAAAMGSHVAAP
                     VLTPNVRYDPATSFVPIGITAHSPAVVIARKDFPARDLKEFVAILQQKGDALKQAHGG
                     IGASSHMSCLLFTKAIGATPTLVAYRGSAPALNDVVGGHVDFLCEQSVAVAESVLGGT
                     VKAYAVSGAERLATLPNVPTAKEMGVPFEMSIWAGLFAPKDTPAEVVAKLAEALDQAL
                     DEPSVRERLGQLGGTIPAKAERSPAAFDRFVRAEIARWAPLLSASKP"
BASE COUNT          371 a          753 c          698 g          361 t
ORIGIN      
        1 catcggctgg tggtcggacg tgttggccga cgccgtgcaa cgctcgggca agctcaagat
       61 tgtcagctgc tacacgcgct cggcggcgaa gcgccaggcc ttcgccgaca aatacgcctg
      121 ccggtcggca gcaagctatg aggcactgct ggccgatccg gagatcgagg ccatcatcaa
      181 caccacgcca aacactgtgc atcggctcac gtgtttggcg gccgcggctg cgggcaagca
      241 cgtgttcctc gacaaaccga tcgccaacag catcgccgac ggccgcgcca tcaccgatgc
      301 ctgccggcag gctggcgtcg tcttggcgct tggctaccaa cggcggcgcg aaagccattt
      361 tcgctggatc aagcagcaga tcgatgccgg cctattcggc cgactggtca atgccgaggc
      421 aaacatcagc cgtgaccggc ttggcaagat tgatcttagc tcctggcgct atcgtgccga
      481 cggcatgccc ggcggcgtga tgctgcaaat cggcatccat tacacggatg tgctggaata
      541 tctgctcggg ccaatcaagg cggtgcgcgg acagctggcg cagttggtgc tgccgggcga
      601 caacccagat gtcgccagtt tgcttttgca gcatgaaagc ggagcgctct cgacgctgaa
      661 cgccagctac gcctcggcgt cggaatatta cttgatgaat atctacggca aggacgccac
      721 cgcatattat gacctgcaca acgggctgcg cctcagccgg cgcggcgaac gcgagcccag
      781 gcgcgtgcca ggcccaacca ccgacacctt ggtcgaagag ctggaggagt ttgccgccgc
      841 gatccgcggc ggcggccaga tcgaggttgg cggcgagtat ggcacgcggt cgctcgcggt
      901 cgtgcgcgcc ggcatacttt cggcacagca agggcgtcag atcgaggttg ccgacctctt
      961 gaagagcgac gccggcgtct agccggccag cgcgggcacg aggagccaag catgaagcca
     1021 cgccgccacg ttcgattgat tgccctgttg ttgcttgccg gcagcctgct accggccgcg
     1081 gcccaggatt atccctcgcg ttcgatcacc gtcatcgtcc ccttcccgcc ggggggcgcc
     1141 agcgatgtcg tggcccgcat cgtcaccgac cagatgtcgc ggctgctcaa gcaagcgatt
     1201 gtcatcgaaa atgtgggagg agccggcggc acgctgggca gcgcgcgggc cgccgccgca
     1261 gcgcccgacg gctacacgct gctcgccgcg gcgatgggat cccacgtcgc cgccccggtg
     1321 ctgacgccca atgtcaggta cgatcccgcc acaagttttg tgccgatcgg catcaccgcc
     1381 cattcgccgg cggtggtcat cgcccgcaag gattttccgg cccgagacct gaaggaattc
     1441 gtcgccatcc tgcaacaaaa gggtgacgcc ttgaagcagg cacacggtgg catcggtgcc
     1501 tcctcccaca tgtcctgcct gctcttcacc aaggcgatcg gcgccacgcc aaccctcgtc
     1561 gcctatcgcg gctcggcgcc ggcgctcaat gacgtcgtcg gtggccatgt cgattttctc
     1621 tgcgagcagt cggtagccgt cgccgaatcc gtgctcggcg gcaccgtcaa agcctacgcg
     1681 gtctcgggcg cggagcgcct ggccacactg ccgaacgtgc cgaccgccaa ggaaatggga
     1741 gtgccgttcg agatgagcat ctgggccggc ctgttcgcgc ccaaggatac gccggccgag
     1801 gtcgtggcca agcttgccga ggcgctggat caggccctgg acgagccgag cgtgcgcgag
     1861 cggctgggcc agcttggcgg caccattccc gccaaggccg agcgcagccc cgccgccttc
     1921 gaccgctttg tccgcgccga aatcgcgcgc tgggccccgc tcctctctgc cagcaagcct
     1981 taagaagcgc agttacagat cgccccgtcg aggccttggg cgttgcccgc cgcgggcagg
     2041 ttcgaccagt gcgcccacgg ccgcccacgc cggctctgcg ctccggcttg cggtcgctgc
     2101 caaccccaac cctgaacgcc gatcccgcac gcttgcttgc gggatttgcc gcccgagccc
     2161 gcctcggctg cgaacgatgg caa
//
LOCUS       DASTMT010000286         1565 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_1462220, whole genome shotgun sequence.
ACCESSION   DASTMT010000286 DASTMT010000000
VERSION     DASTMT010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 1565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1565
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1462220"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..724)
                     /locus_tag="VFR00_06600"
     CDS             complement(<1..724)
                     /locus_tag="VFR00_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018640811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0158961.1"
                     /translation="MASCRHMPSRRAALLGSGALFAWTQLPKLARAEGRDPRLIVIVL
                     RGALDGLGAVAPVGDPDWIRLRGERALVLDGKPPALPLDSFFALNPAMPNLHRLYRAE
                     QATIVHATATPYRERSHFDGQDVLESGLAKPGALDSGWLNRALLGLAPAGGVALQDKR
                     AFAVGPVTPLIVRGQAPVMSWSPQQLTPASDDTRARLLELYQHADPKLAQALEGRIRL
                     AALARGEGAVGNATPLPPAGIER"
     gene            complement(728..>1565)
                     /locus_tag="VFR00_06605"
     CDS             complement(728..>1565)
                     /locus_tag="VFR00_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008132757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1800 domain-containing protein"
                     /protein_id="HEU0158962.1"
                     /translation="MGPNSVAGLNRTRGLNENLAREILELHTLGVRTVYSQDDVIRFA
                     NVLTGWTLLPAGDNPEHGGEFTFNRRLHEPGPHVVLGKSYPEGGVEQGKAVLNDLAQH
                     PATAKHVAAKLARHFLADDPPPALVETLARTFQDTAGDLKEIMKALIRAPEALSLPPS
                     KLRRPSEWVTAIVRAAGTTADPRRYTGGQATLGEPLWRPPSPKGHADDEAAWIDGMGQ
                     RLDIANTYAERIVGRVDPRLIMDSVLAGTLSETTTAVVGRAESRQQALALLFMSPEFQ
                     RR"
BASE COUNT          252 a          522 c          548 g          243 t
ORIGIN      
        1 cccgttcgat ccctgccggg ggcaaggggg tggcgttgcc aacggcgccc tcaccacgcg
       61 ccagcgccgc caggcgaatg cgtccctcca gcgcctgagc cagcttggga tcggcatgtt
      121 ggtagagctc caacaggcgc gcgcgcgtgt cgtcgctggc cggggtcagc tgctgcggtg
      181 accaggacat gaccggtgcc tgcccgcgca cgatcagcgg cgtcaccggt ccgacggcga
      241 aagcacgctt gtcctgcagc gcaacacccc cagcaggggc gagccccagc agggcgcgat
      301 tgagccagcc gctgtccaag gctccgggtt tggccaaacc gctctccaac acgtcctggc
      361 cgtcgaagtg tgagcgctcg cggtagggcg tagcggtggc gtgcacgatg gtggcctgct
      421 cggcgcggta gagccggtgc aggttgggca ttgccgggtt gagggcaaag aacgaatcca
      481 gcggcagcgc cggcggcttg ccgtcgagca caagcgcacg ctcaccgcgc aagcggatcc
      541 aatccggatc gcccaccggt gccaccgcac cgagaccatc cagcgcgccg cgcaagacga
      601 tgacgatgag gcgtggatcg cggccttcgg cccgtgccaa cttcggcagt tgcgtccagg
      661 caaagagcgc accggaaccc aagagggcgg cgcggcggct cggcatatgc cggcaagaag
      721 ccatgggtca cctccgctgg aattcagggg acatgaacag aagcgccagc gcctgctggc
      781 ggctctcggc gcgaccaacg acggccgtcg tggtctccga tagggttccc gcgagcacgc
      841 tgtccatgat caggcgcgga tcgacccggc cgacgatgcg ctccgcatag gtgttggcga
      901 tgtcgaggcg ctggcccatg ccgtcgatcc aggcggcctc gtcatcggca tgacccttgg
      961 gggaaggcgg acgccacaaa ggttcgccga gggtcgcctg ccccccagtg tagcgccgcg
     1021 gatcggccgt ggttcccgcc gcgcgcacga tcgcggtcac ccattcgctg ggacggcgca
     1081 gcttgctcgg cggcaagctc aaggcctccg gcgcgcggat caaggccttc atgatttctt
     1141 tcagatcccc cgcggtgtcc tggaaggttc gcgccagggt ttcgaccagg gccggcggtg
     1201 gatcatcggc caggaaatga cgcgccagct tggctgcgac gtgcttggcc gtggcgggat
     1261 gctgcgccaa atcgttgagc acggccttgc cttgctcgac cccaccttcg ggatagctct
     1321 tgccgagaac gacgtgcggg ccgggctcgt gcaggcgccg gttgaaggtg aactcgccgc
     1381 cgtgctcggg gttatcgccc gccggcagca gggtccaacc cgtcagcacg ttggcaaagc
     1441 ggatgacgtc gtcctggctg tagaccgtgc gcaccccgag cgtgtgcagc tcgaggattt
     1501 cgcgggcgag gttttcattc aggccccgcg tgcgattgag accggccacg gaattcgggc
     1561 ccatc
//
LOCUS       DASTMT010000287         2491 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_6313116, whole genome shotgun sequence.
ACCESSION   DASTMT010000287 DASTMT010000000
VERSION     DASTMT010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2491)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2491)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2491
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6313116"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(12..1268)
                     /locus_tag="VFR00_06610"
     CDS             complement(12..1268)
                     /locus_tag="VFR00_06610"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEU0158963.1"
                     /translation="MRPIRIAIAGAGTAGLAAAAFLARDGHLVELFERFEEAKPLGAG
                     LLLQPTGLACLAKLGLDGTALDYGQAVTSIYGETSRGVPILDLSYRELGAHVFGLGIH
                     RGALFSLLHAEVARLGITLTTATEIACSRATAEGRILIDRQHNEHGVFDLLVDATGVR
                     SPLRATDASIRRERPYRYGAVWGSLPEPADWPHRDRLVQRYEGCHSMVGMLPIGRAPG
                     DSRRLIALFWSLRVEDRARWQAAGVEAWKAQVIEVCPQAEPLLAKINSLDEMTFASYA
                     DLQLGRCRGPRIVFIGDAGRALSPQLGQGANLALIDAATLADSLRQASSIEAALGLYA
                     TARRRHGNFYALASRWLTPFFQSDSRWAARLRDVAFPLMAKLPYLRRQMAQTLAGMKT
                     GLFSELDPGRWHAKYALKRPDAPPAA"
     gene            complement(1337..>2491)
                     /locus_tag="VFR00_06615"
     CDS             complement(1337..>2491)
                     /locus_tag="VFR00_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004446645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEU0158964.1"
                     /translation="FTCTSAHTCTGDGNAMVLRAGLPLEDMEFVQFHPTGVYGAGVLI
                     TEGARGEGGYLTNANGERFMERYAPHVKDLASRDVVSRAMTIEIREGRGVGPEKDHIF
                     LHLDHLDPKILHERLPGITESAKVFAGVDLRREPIPVLPTVHYNMGGIPTNYHGEMLT
                     LKGGNPDYVVPGLMALGEAACVSVHGANRLGSNSLIDLVVFGRAAGLQCAKTIEPGGA
                     QPDLPGDAGELALARLDRFRHANGGTPTAALRLKMQRIMQMNCSVFRDGPVLKEGLAK
                     ITDVWKGVSDIRVSDRSLIWNSDLVETLEFDNLIAQAAVTVAAADNRTESRGGHARED
                     YPERDDENWMKHTLTWADAAAQTVRIDYRPVHTYTLTNEVNYFPPKPRVY"
BASE COUNT          415 a          777 c          871 g          428 t
ORIGIN      
        1 cgcccggcgc gtcaggcggc tggcggggcg tcggggcgct tgagggcgta cttggcatgc
       61 cagcggcccg ggtcgagctc gctgaatagg cccgtcttca tgcccgccaa ggtttgcgcc
      121 atttggcgcc gcagataggg cagctttgcc atcagcggga aggcgacgtc gcgcaggcgg
      181 gcggcccagc ggctgtctga ctggaagaag ggcgtgagcc agcggctggc cagggcgtaa
      241 aaattgccgt gccggcgtcg cgccgtcgca taaagaccga gcgctgcctc gatcgagctg
      301 gcctggcgca ggctgtcggc gagggtcgcg gcatcgatca gggcgaggtt ggcaccttgg
      361 ccaagctgtg gcgaaagcgc ccgtccggca tcgccgataa agacgatgcg gggtccgcgg
      421 caccgaccca attgcaggtc ggcatagctc gcaaacgtca tctcgtcgag cgaattgatt
      481 tttgccagca gtggctccgc ctgcgggcag acctcgatca cctgggcttt ccaggcctcg
      541 acgccggccg cctgccaacg ggcgcgatct tcaacacgca agctccaaaa cagcgcgatc
      601 aggcgccggc tatcgcccgg cgcccggcca atgggcagca tgccgaccat gctgtggcaa
      661 ccttcgtagc gttgcacgag ccgatcgcgg tgtggccagt cggcaggttc tggcaggctg
      721 ccccagacgg cgccataacg atacggccgc tcccgccgga tgctggcgtc ggtggctctg
      781 agcggcgagc gcacgccggt ggcgtcgacg aggaggtcga agacgccgtg ctcgttgtgc
      841 tgtcgatcga tcagaatgcg gccttcggcc gtggcgcggc tgcaggcgat ttcggtcgcg
      901 gtggtgagcg tgatgccaag acgtgcgacc tcggcgtgca acaacgaaaa gagcgcgccg
      961 cgatgaatgc cgagaccgaa cacgtgtgct cccagttcgc ggtaactgag gtcgaggatg
     1021 gggacgcccc tgctggtctc gccataaatg ctcgtgacag cctggccgta gtcgagggca
     1081 gtgccgtcga gccctaactt ggcgagacag gcaaggcctg tgggctggag caggaggccg
     1141 gccccgagcg gcttggcttc ctcgaaacgc tcaaacagct caaccaggtg cccgtcacgc
     1201 gcgaggaagg cggcagcggc cagcccggcg gtgccggcgc cggcaatggc gatcctgatt
     1261 ggccgcatcc cccgcgttgc ctccacgccg tgccggcccg gaccggactg gccagctgac
     1321 cttgcgcgct ttggctctag tacacccggg gtttgggtgg gaagtagttg acttcgttgg
     1381 tcaaggtgta ggtgtggacg ggacggtaat cgatgcgcac cgtttgcgcc gcagcgtcgg
     1441 cccaggtcag ggtgtgtttc atccagttct cgtcgtcccg ctcgggatag tcctcgcgag
     1501 cgtggccgcc tcggctttcc gtgcggttgt cggcggccgc caccgtgacg gcggcctggg
     1561 cgatcaaatt gtcgaattcc agggtttcga ccagatcgga gttccaaatc agcgagcggt
     1621 cggaaacgcg aatatcgctg acccccttcc agacatcggt gatcttcgcc agaccttcct
     1681 tgagaacagg cccgtcgcgg aagacagagc agttcatttg catgatgcgc tgcatcttca
     1741 ggcgcagcgc ggccgtgggc gtgccgccgt tggcgtgccg gaagcgatcg aggcgcgcaa
     1801 gcgccagctc gccggcgtcg ccgggaaggt cgggttgggc accgccaggc tcgatggttt
     1861 tggcgcactg cagaccggcg gcgcgaccaa acaccaccag gtcgatcagc gagttgctgc
     1921 ccagccgatt ggccccatgc acggagacgc aggcggcttc gcctagcgcc atcaggcccg
     1981 gcacgacgta atcgggattg ccgcctttga gcgtcagcat ctcgccgtga tagttggtgg
     2041 gaatgccgcc catgttgtag tgcacggttg gtaggaccgg gatcggctcg cgacgcaaat
     2101 cgacgccggc gaagaccttg gcgctttcgg tgatgccggg cagccgttcg tgcaggatct
     2161 tggggtccag gtgatccaaa tgcaggaaga tgtgatcctt ttccggccct acgccgcggc
     2221 cttcccggat ctcgatggtc atggcacgcg agacgacgtc gcgcgaggca aggtccttca
     2281 cgtgcggcgc gtagcgctcc ataaaacgct cgccgttggc gttggtgaga tagccgccct
     2341 cgccgcgcgc accctcggtg atcagcacgc cggcaccata aacgccggtc gggtgaaatt
     2401 gcacgaactc catgtcctcg aggggcaggc cggcgcgcag gaccatggca ttgccgtcac
     2461 ccgtgcaggt atgcgcggag gtgcaggtga a
//
LOCUS       DASTMT010000288         3499 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_1791839, whole genome shotgun sequence.
ACCESSION   DASTMT010000288 DASTMT010000000
VERSION     DASTMT010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3499)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3499)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3499
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1791839"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..336
                     /locus_tag="VFR00_06620"
     CDS             <1..336
                     /locus_tag="VFR00_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014491722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase"
                     /protein_id="HEU0158965.1"
                     /translation="ACPFGAPQYPKLGNFGSRGKMDKCTYCAGGPEADLTAAEYGSRE
                     NGQFTGYGSNRLAEGKLPLCAEMCSTKSLLAGDGEIIAQIYKERVVRRGYTSGAWGWQ
                     TAYRETIAS"
     gene            423..1424
                     /locus_tag="VFR00_06625"
     CDS             423..1424
                     /locus_tag="VFR00_06625"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019173740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit gamma"
                     /protein_id="HEU0158966.1"
                     /translation="MARMGPILRSLIGALALCLLAVATPPAVAQQPSSVNPTAQSVKE
                     QELLKELQRIQGRSSLPDARTRVLEQPAGRDWRHFHEVTLPWIGAIAILGMLAVLILF
                     YLFRGMVRIEGGRSGRTLVRFNAFERFVHWMTATCFIVLALSGLNVTFGKQLLTPLIG
                     PEGFAAWAQWAKYAHNYLSFPFSIGVALMFLLWIAWNFPSGTDVHWLREGGGIVGKHH
                     PPARRFNAGQKMIYWVVVLGGGAAAITGYLLMFPFYATDVTGMQTAQMVHGIVGMLFI
                     AIMLAHIYIGTIGMQGAFEAMGKGEVDVNWARQHHSLWLENEIRKGRASGGSVAPAE"
     gene            1703..2311
                     /locus_tag="VFR00_06630"
     CDS             1703..2311
                     /locus_tag="VFR00_06630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0158967.1"
                     /translation="MQKLSGALALGWCIVIAGTFAAAGSAGAQAPAAANQEEIKKQDS
                     VPPATAPKLTEQSGKTEPSAKIEGTHPNSVFVNGVLDVPGANTDIDTAPAKHWARTNA
                     DDQIPIAGYRLKKLDPQQLRQIAQELTSQREAPAASPAPGNYAVIGAEVPANVALAAL
                     NPVPQTLTDKFVELRGTAFMRSAGKLVIVDPKNNLVLGVLEG"
     gene            2384..2509
                     /locus_tag="VFR00_06635"
     CDS             2384..2509
                     /locus_tag="VFR00_06635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0158968.1"
                     /translation="MRTFLVAVLAAVVIAATAAIVLNTYVPDTSAGAFSTQGVRI"
     gene            2875..3060
                     /locus_tag="VFR00_06640"
     CDS             2875..3060
                     /locus_tag="VFR00_06640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0158969.1"
                     /translation="MREKISLIAATALITALITAWSMHALGTSAPAKTTFAPASMNIM
                     QMMRNAKDLPSEHYDAF"
BASE COUNT          638 a         1200 c         1075 g          586 t
ORIGIN      
        1 gcgtgtccgt tcggcgcgcc gcaatatccc aagctcggca acttcggctc gcgcggcaaa
       61 atggacaagt gcacctactg cgccggtggt cccgaggcgg acctgaccgc ggccgagtat
      121 ggcagccgcg agaatggtca attcaccggc tatggctcga accgactggc cgaaggcaag
      181 ctgccgctgt gcgcggagat gtgttcgacc aagtccttgc tggccggcga tggcgagatc
      241 atcgcgcaaa tctataagga gcgcgtggtg cggcgcggct acacctcggg ggcctggggt
      301 tggcagaccg cctaccgcga gaccatcgcc tcctgagagg cgtagtcgcg cgaagcgggg
      361 ttgccgcgcc tgcggccacc ccacaagaac acttgaggca agacaatagg gagagttcgg
      421 caatggcgcg catgggccca atattgagat cgttaatcgg cgcgctcgcc ttgtgcctcc
      481 tcgccgttgc gacacccccc gcggtcgcgc aacagccgtc ctcggtcaat ccgacggcgc
      541 agtcggtcaa ggagcaggag ctgctcaagg agctgcaacg catccagggc cgcagctcgc
      601 tgcccgacgc ccgcacccgg gtcctcgagc agcccgccgg ccgcgactgg cggcacttcc
      661 acgaggtgac gttgccctgg atcggggcga ttgccatcct cggcatgctg gcggtgctga
      721 tactgttcta cctgttccgc ggcatggtgc gcatcgaggg cgggcgctcg gggcgcacgc
      781 tggtgcggtt caatgccttc gaacggtttg tgcactggat gacggcgacc tgcttcatcg
      841 tgctggcgct cagcggtctc aacgtcacct tcggcaagca gctgctcacc cccttgatcg
      901 ggcccgaagg ctttgccgcc tgggcccaat gggcgaaata cgcccacaac tacctgagct
      961 tccccttcag catcggtgtg gctttgatgt tcctgttgtg gatcgcctgg aacttcccgt
     1021 ccggcaccga cgtgcactgg ctgagggaag gcggcggcat cgtcggtaaa caccatccgc
     1081 cggcgcggcg cttcaacgcc gggcagaaga tgatctactg ggtggtcgtg ctgggcggcg
     1141 gcgccgccgc catcaccggc tatctcctaa tgttcccctt ctatgccacc gatgtcacgg
     1201 gcatgcagac ggcgcagatg gtgcacggca tcgtgggcat gctgttcatc gccattatgc
     1261 tggcccatat ctacatcggc accatcggca tgcagggcgc cttcgaggcc atgggcaaag
     1321 gcgaggtcga cgtcaactgg gcccgccagc accacagcct ctggctcgag aacgaaatcc
     1381 gcaaaggccg cgccagtggc ggcagcgtgg cgccggccga ataacgggcg ccaacgtttc
     1441 gcttgcttta aaaggtgaat cgaacggggc gagccccgtt cgtgcccgcg cctgcctgtc
     1501 tcgcggcatg cgggccaaaa cgcgcgctta agcgaaaagc gcgcaatctc gctcgccagc
     1561 gcagccctgc ccgcggcgag ggcggaacat tcattccgca cttgacgttg ttgcctcaaa
     1621 tcacctgggg cctgcgcctg cgccaagggc gtcggcacga aaggcaggtg accgcaccct
     1681 tcgagcgtaa gaggtgatga tcatgcaaaa gctttctggc gccttggccc tcggctggtg
     1741 catcgtcatc gccggcacct tcgccgctgc ggggagcgcc ggcgcccagg cccccgccgc
     1801 tgcaaaccag gaggaaatca agaaacagga ctcggtaccc cccgccacgg cgccgaaatt
     1861 gacggagcag tcgggcaaga ccgagccaag tgccaaaata gaaggcactc accccaatag
     1921 cgtgtttgtc aatggcgtgc tcgacgttcc cggcgcgaat accgatatcg acaccgcccc
     1981 cgccaagcac tgggccagga ccaacgcgga cgatcagatc cccattgccg gctaccggct
     2041 gaaaaagctc gacccccagc agttgcgcca gatcgcccag gagctgacct cacagcggga
     2101 ggctccggcg gcctctccag cccctggcaa ttacgccgtg atcggcgccg aggtccccgc
     2161 caatgtggcg ctcgccgcgc tcaatccggt gccgcagacc ctgaccgaca aattcgtcga
     2221 actgcgcggc acggccttca tgcgctctgc cggcaagctc gtgatcgtcg atcccaagaa
     2281 caatctcgtc ttgggcgtgc tcgagggtta gccccgcgcg acccgccact tccaggcccc
     2341 tcctactctg tgctagaaca aacggaaagc ctgggaggag actatgcgca cgtttctcgt
     2401 cgccgtgctg gccgcggtcg ttatcgccgc caccgctgcg atcgtcctca acacctacgt
     2461 accggatacg tcggcggggg ccttttccac ccagggtgtc cgcatctagc agcgacacgt
     2521 gagctcgtga gccaaggccg ctcgcgccga ccgccacagg gcgtcagagc aaacccgaag
     2581 ctccctgctg tcccccatca cctcgggggc agcagcctcc ctcatctttc tccactgccg
     2641 cggcagcggg ctttgctgcg ggctcctgcc gacgccgcta caccagaagc ggtttgaggc
     2701 accggcaagg cgctcgcagc agcgcgacct cacccgaccg catgccgttt tttccctgct
     2761 gtcactttgt cttgtgccac agagcacagc gccgcctcgc gttttgagcg atgagtgccg
     2821 gcacagcatt tgacgtgctg gctatccgtc gtcccgcacc cgaggagacc gaacatgcgc
     2881 gaaaaaatct cgttgatagc agctaccgcg ctcatcactg ccctcatcac ggcttggagc
     2941 atgcacgcgc tcggtaccag cgcgccggcc aaaaccacgt tcgcccccgc gtccatgaac
     3001 atcatgcaaa tgatgcgcaa cgccaaagac ctgccgagtg aacactacga cgctttttaa
     3061 gagcggccac gcgcgtcggt tgggtgccga tgccgccagc aaagggctgg cgcgcttgcc
     3121 gggccgcccc atgcgaacga ccgagcaccc ccgctgaggc ggccgcccgt tggccggaaa
     3181 gaccggaaag accgggcaag aactggcaaa cttcgaagat cggcacgaaa ccgcgcgcca
     3241 cctagcatcc ctccatcggc tcacacgtcg tgggcgccaa aagatggagg tgaagtcatg
     3301 aaggctgcga tactcgcttt gctcttgctc ttggctggcc tttcggggct cgcgtacagg
     3361 gccccggcct gggccggcag ctgcagccac tgcgttttcg acgacgaacc cccgcttccg
     3421 ccgcaaccca agcgcgccaa cccgggctag cggccttgcg agtgtggcac gtcacggcga
     3481 ggcctgccga ggtcgtcga
//
LOCUS       DASTMT010000289         2323 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_812298, whole genome shotgun sequence.
ACCESSION   DASTMT010000289 DASTMT010000000
VERSION     DASTMT010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2323
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_812298"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1504
                     /locus_tag="VFR00_06645"
     CDS             <1..1504
                     /locus_tag="VFR00_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007751923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEU0158970.1"
                     /translation="ITANVPTQQFNRSPFQEINRHFQADFPNIIRPVVKRSFQPTRVD
                     MLPLALRQAMTAMLSGRPGPVNLDVPFNVFQEEDEVDMPAVAPVHGSQRSGAAPEQVA
                     AALDMLLAAKRPVAFIGHGVTLSEASAELTHLVDALELPVISSPNGMGALDMAHPLSL
                     GFIGRNGAYPANEAGRRADLVLAIGARFDDRSASSWIPGYSWNFPHAKLIHVDVDWEE
                     LGRNYPPDLGVIADARTFLRQLLAELAQRPVRPKAALRDWHAAIKDWAAAWEAYTRPN
                     FEIHASPLRPERVVADCQAVLPPDAVLACDVGVNHNWFMQFWQARRPQTMLNSWGFSG
                     MGFGAAGVLGAKLAAPERPCVAIAGDGSFTMVPHVLLTAVEYDIPVVWVIWNNFGWVS
                     IRDIQLGMFGGRELGTMFRQGANNTPYNPDFAAWAKASGVDALTVTRSQDFKGALEHA
                     IKANKPFLLDVHVDAEVRPPATGTWQLPPTPYREPAFGRRWTPGEPGPQA"
     gene            1681..2058
                     /locus_tag="VFR00_06650"
     CDS             1681..2058
                     /locus_tag="VFR00_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009450401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEU0158971.1"
                     /translation="MTESKPGVLIRNIAEVPWRQFPDHFGGALSKPLVLPETAGSRHL
                     DYRISMYQPMAYVALHAHKVQEQIYHVLEGEGLMQIDGKKHVVRKHDVIYLPPGIPHA
                     IENSGLVDLVFLVVTSPVTDEAP"
     gene            2067..>2323
                     /locus_tag="VFR00_06655"
     CDS             2067..>2323
                     /locus_tag="VFR00_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018386910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HEU0158972.1"
                     /translation="MQLIIFDCDGTLVDSQHKICAAMTHAFTALQLPVPSRSDILGIV
                     GLSLPQVFAVLAANEPLSVQARLAELYAQGFPAHRPGAPLN"
BASE COUNT          370 a          775 c          757 g          421 t
ORIGIN      
        1 catcaccgcc aacgtgccga cccaacagtt caatcgcagc ccgtttcagg agatcaaccg
       61 gcactttcag gcggatttcc ccaacatcat ccggcctgtg gtcaagcgct cattccagcc
      121 gacccgcgtc gacatgttgc cgctcgcctt gcgccaggcc atgacggcca tgctgtcggg
      181 tcgaccggga ccggtgaacc tggatgtgcc cttcaatgtc ttccaggaag aagatgaagt
      241 ggacatgccg gcggtggcgc cggtgcatgg cagccagcgc agcggtgcgg cgcccgaaca
      301 ggtggcggcc gcgctcgaca tgctgctggc ggccaagcgt ccggtggcgt tcatcggaca
      361 tggcgtcacc ttgtcggagg caagcgccga gctgacccat ctggtcgatg ccctcgagct
      421 gccggtgatc tcctcgccca acggcatggg tgcgctcgac atggcccacc ccttgtcgct
      481 gggcttcatc ggtcgcaatg gcgcctatcc cgccaacgag gccgggcggc gcgccgatct
      541 ggtgctggcc atcggggcgc gcttcgatga tcgctcggcg tcctcttgga tccccggcta
      601 ctcctggaat ttcccgcacg ccaagctcat tcatgtcgac gtcgattggg aggaacttgg
      661 acgcaactac ccacccgact taggcgtgat tgccgatgcc cgcaccttcc tgcgccagct
      721 gctggcggag ctggcgcagc gtcccgtccg gccgaaggcc gcgctgcggg attggcacgc
      781 cgcgatcaag gattgggcgg cggcttggga ggcctatacg cgccccaact tcgagatcca
      841 tgcgagccca ctgcggcccg aacgcgtggt ggcggattgt caggcagtgt tgccgcccga
      901 tgcggtgctc gcctgcgatg tcggtgtcaa ccacaactgg ttcatgcagt tctggcaggc
      961 gcgccgaccc cagaccatgc tcaactcctg gggcttctcc ggcatgggct tcggcgcggc
     1021 gggtgttctg ggcgccaagc tggcggcccc ggagcggcca tgcgtcgcca ttgccgggga
     1081 cggctcgttc accatggtgc cgcacgtgct gctgacggcg gtcgagtacg acatccccgt
     1141 ggtgtgggtg atctggaaca atttcggctg ggtctcgatt cgcgatattc agctcggcat
     1201 gttcggaggg cgtgagcttg gcaccatgtt ccggcagggg gccaacaaca ctccctacaa
     1261 cccggacttc gcggcctggg ccaaggccag tggtgtggat gccctcaccg tcacccgctc
     1321 gcaagacttc aagggcgcgc tcgagcacgc catcaaggcc aacaagcctt tcctgttgga
     1381 cgtgcacgtc gacgccgagg tgcggccgcc ggcgacgggc acgtggcagc tgccgcccac
     1441 gccttaccgc gagcccgcct tcggtcggcg ctggacgccc ggggagcccg ggccgcaagc
     1501 ctagggggcg ggcgagagtg ctcgcctgcc gtcgcaaccc gtccctggca tggtcgccaa
     1561 gccgtacgtt gacgctggtg ccgggctcgc gcatggtccg cccggcggcc ggacgccaag
     1621 gcctttgtcc gccgcagcag acccgagagg gatccgagag cagtgggagc ccagagcgcg
     1681 atgaccgaat ccaagcctgg cgtcctcatc cgcaacatcg ccgaggtgcc ctggcggcag
     1741 ttccccgacc acttcggcgg ggcgctgtcg aaaccgctcg tgctgcccga gacggccggt
     1801 tcgcggcatc tcgactatcg catatcgatg taccagccga tggcctatgt tgcgctgcat
     1861 gcgcataagg tgcaggagca gatctatcac gtgctcgagg gcgaagggct gatgcagatc
     1921 gatggcaaaa agcatgtggt gcgaaagcac gacgtgatct atctgccgcc gggcattccg
     1981 catgccatcg agaattccgg gctggtcgat ctcgtttttc tggtggtgac ctcgccggta
     2041 acggacgagg ctccctgacg tcggccatgc agctcatcat cttcgattgc gacggaacgt
     2101 tggtcgacag ccagcataag atttgcgcgg ccatgaccca tgcctttacg gctctgcagc
     2161 tgccggtgcc gtcccgcagc gacattttgg gcatcgtcgg cttgtccttg ccgcaggtgt
     2221 tcgccgtgct tgccgccaat gagccgctga gcgtgcaagc gaggctcgcc gagctttacg
     2281 cccaaggttt tccggcgcac cgccccggcg caccgctcaa cga
//
LOCUS       DASTMT010000290        13768 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_1141052, whole genome shotgun sequence.
ACCESSION   DASTMT010000290 DASTMT010000000
VERSION     DASTMT010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 13768)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13768)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13768
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1141052"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..579)
                     /locus_tag="VFR00_06660"
     CDS             complement(<1..579)
                     /locus_tag="VFR00_06660"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU0158973.1"
                     /translation="MTGKPMLEVFELCAGYLPGVEILSGLSLSAAENAVTLVIGPNGA
                     GKSTLLRTIFGLLAPRAGRVAIAGSDVTGAGPSRLKAAGVSYITQDINSFPYLTVEEN
                     LLMGAWTFRRDRARIARQLEHLYHLFPVLRERRGQRAGALSGGQGRMLSVARELMTAP
                     QLLLIDEPTVGLAPNLVAQVYATLAAARSATGA"
     gene            complement(576..1310)
                     /locus_tag="VFR00_06665"
     CDS             complement(576..1310)
                     /locus_tag="VFR00_06665"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU0158974.1"
                     /translation="MPALLRAQDLSKRYGGLVANNKVSLSLGPGEIRGLIGPNGAGKT
                     TLVNLLTGIETPDAGRVWLGNVELSGLGAHQIAAHGLVRSFQVARIFESLSVSDNLVL
                     PFLAAGSPGGLAAGLARVRELLALATLSPLAQERAGTLSGGQRMLLQACAGFMVPSVT
                     LYVLDEPFAGINPVVKDTLIALIERENSEHHRAFLIVSHEMEIVRRLCPQVSVMIEGR
                     IAAEGTLEEVARRPEVIAAYLGRSAA"
     gene            complement(1310..2206)
                     /locus_tag="VFR00_06670"
     CDS             complement(1310..2206)
                     /locus_tag="VFR00_06670"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEU0158975.1"
                     /translation="MIFSAELLIQTLLSGLMVGVLYALMALGITFIYSIVKTINWAMG
                     EFYMIGSYIQFVAVHFLLGPNWWWLAAILAASGTFLLGLILEPILIKPMYARPLQRRD
                     DYATVVTIALLLLLRNLAVALGGPNQYRPESTLPVVWVGPLPQEGARVAASIFAVLAL
                     GSFWLVIKKTWVGLALRAAAQNRVAVQTAGVDVFRLDAIAFAIGVALAGFAGALLAPL
                     FMVFPTNGAVTTVKGFEIIVIGGLGSIPGALIGGLLLGLVESLGAAFISSPYQNVYGF
                     LLVILVLILKPNGLFGERGREA"
     gene            complement(2274..3449)
                     /locus_tag="VFR00_06675"
     CDS             complement(2274..3449)
                     /locus_tag="VFR00_06675"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0158976.1"
                     /translation="MRRGGFAGGALLVSMLWGLGQACAQEPIVFGVITPLSPPGETAL
                     GQLAKRGAEIGAEYINEKGGVLGRKVALSVHDSAGKNEQAVAAYRRLVSSEKAVAVFG
                     FIHSGANIAVNEVAKEMGVPTMGTQTGASDVTAKRYDIAFRTHAVDPPRAATWLGWIK
                     KNNWKRVSLIAETTDYGIGLAKETEKQNKDKNLGLEIQTIMFDRTTTDLLPQLLQVKA
                     FKPDAIVNVGVGQPLDLMITQAHTVGLLPGTAMVTSYDAPARPQFWQLHGDKGVGVSF
                     IAFYSPKSELSEVGKEFAKRYQAKFNEPPVYAALNGFANALVFAAAIEQAKSTDPKAM
                     IKALEEGSFTGWSTTPVTFPRAEGALWHNWSPPLMVLTYSAKDQTPTTATVGYVLGE"
     gene            complement(3559..4650)
                     /locus_tag="VFR00_06680"
     CDS             complement(3559..4650)
                     /locus_tag="VFR00_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011582954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0158977.1"
                     /translation="MARACDILIFGSGSFAQRIACDLAATATEPIALGLAGRNSARLD
                     WIATAARARAHMFGRPLRLTSYAVDLGAAGAAETLLAKLEPGVVVQAASQQGGAVIAA
                     GGTAWGRLVAEGGLSATAVFQALLSARVARAMRELALQGQLINCCFPDVVNGILQGMG
                     LPVLCGIGNIGILAHAFAGSLAAAEPAPDLKLLCHYQAIAAWRRPRAERTGPAPRVWL
                     DGREIDDVFARFAPVQLTPEPVIDISGASGVPLMLALAKGGAWRGHVPGPAGLPGGYP
                     VVVAAGKLDLDLPAGLARADAVAWNARFEAENGLFIGADGRAEFRGRLRERLAAASPA
                     LAAGFPVADLEAVAADFGELRDRLLARPA"
     gene            4756..5979
                     /locus_tag="VFR00_06685"
     CDS             4756..5979
                     /locus_tag="VFR00_06685"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU0158978.1"
                     /translation="MVQRSADVAIIGGGLVGCFAAYFLRRRGQSVVVIERGAVCTAAS
                     GTNFGNLRLQGRHPAEFALALRAQAIWEELAALSGEGRAIRASGHLYLGLAVSDQPKL
                     ESIAQEANAAGLAVDLLDGNAVRRRWPMVSDLITGACWSRRDGIAEPTRIGPTVARLA
                     AAAGAQLIENTRVTRMVPGAGGFTVSTDRDLSVSCGRLINAAGAWGAELAGACGEPVP
                     LFAAGPPLFEVHPEHAWDGPSLAAVDGTLLLRPGLTGPAVAGTFPRVKADLGSGRAPV
                     PPERVGPALAHLNSVVPGLGRLRAGRVWSGVEGYLPDMLPVIGASGTTPGLVHAFGFS
                     GHGFQLAPGVGAVLAELVTTGVSETPIAAFALARFADGAAVVDDKLWREFDPELVARF
                     RRGHAGTADASRHDL"
     gene            6041..6856
                     /locus_tag="VFR00_06690"
     CDS             6041..6856
                     /locus_tag="VFR00_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEU0158979.1"
                     /translation="MSDAHPVLTDLSDGLATITLNKPAKLNAWDTPMRAEIAAILKAW
                     NGDDRVRAVIVTGAGERAFSAGQDLDETEKFSSGDDGANWFQSWRDFYNAIRDLDKPC
                     VAALNGVAAGSAFQAAMLMDVRVGHRGVRMGQAEINSGIPSVTGPMLMLSRIGLSRTI
                     ELTLTGRMMEAEECCAVGLMHNMVERREEVLPKAREVALLLAAKPVIAMRLTKARFRQ
                     VTQPAFDEAFANGGVYQAEAFASGEPQAAMRTFFAERAARRKARAAGSTSPST"
     gene            6875..8416
                     /locus_tag="VFR00_06695"
     CDS             6875..8416
                     /locus_tag="VFR00_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEU0158980.1"
                     /translation="MAPAPAPFPDTDAGFVGLFDAQARATPQRTFATLDGEALSFGAL
                     AETSARLGAWLDALGVAAGDRVALMLRNGEAALSLMLALARTGRVWVPINPQAVGDSL
                     AYVLQHAEPRLVIAEAELLPAIGASGVNLGERLLEVEAARCAAANLPAKDPRPLPAAD
                     APFAIMYTSGTTGRPKGVIVSHRMLRLSGEAAALVAAPGPGDVFYVWEPLFHIGGAQL
                     LVLPLIRAVRLALAQGFRARSFWADVRAAQATHMHFLGGILQILLKQPEGPFDRDHGV
                     RIAWGGGCPAEIWAAFEARFGLKIRECYGMTECSSITTANLDGPIGSVGKPVPWFDVT
                     ILDPAGQPVRAGMRGEMVVTERMPGALTEGYFRDREATAQALHQSGVFRTGDLASCDG
                     DGNVYFHGRLTDSVRVRGENVSAAEVESVVRQHPAVEDCAIIAVAAEVGEAEIKLFVT
                     LRPGGQLGAAELSGWLTGRLARYQRPRYIAVLDAFQRTPSTRIMKHVLSRRLDDCWDA
                     GPARA"
     gene            8489..9757
                     /locus_tag="VFR00_06700"
     CDS             8489..9757
                     /locus_tag="VFR00_06700"
                     /inference="COORDINATES: protein motif:HMM:NF018682.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HEU0158981.1"
                     /translation="MPEVFILADDLSGAADCAGGCAAAGLDTVVLLAPDLAARARVIA
                     IDLDTRESWAGEAGLTMARTLERAWDPNIKVIYHKIDSTLRGAWAHELAAARQSLAAM
                     IGSAPLALIAPAFPARGRTTRAGRVLVEASSSKPVAVARDAGVIAAPLRQLGLDVRSL
                     ARGEVNATTPALAQRFAALARAKVDAVVCDAETEQDLAAIAAAGLASSLPLLWVGSAG
                     LMRPLAAALADSDTRPVALPAVRGPLLFVVGSAAAAARAQAEVLAAEPDITVIRLRPE
                     DLMQGHAALAAALEAGLDRGRDIALLIDPASPAHGPLDPALVARLADIAGPRLARTGG
                     LVATGGETARRLLERACISSIKLGGEMEVGIPWGIALGAPARQPQLLEAEASNVIGWE
                     PSFPIVTKAGGFGDPGTLVRCRQVLKTKER"
     gene            9800..10819
                     /gene="pdxA"
                     /locus_tag="VFR00_06705"
     CDS             9800..10819
                     /gene="pdxA"
                     /locus_tag="VFR00_06705"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003576232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HEU0158982.1"
                     /translation="MRPIIAITMGDAAGIGPEIIMRALAHAELYEGCRPLVVGDAERL
                     RRAGGLVGSNVTVRALADPRQARFTPGTLDCIDVGLIPADLPWGKVSPIAGDAAYRYI
                     ERAVALAMSKRIDAICTAPLNKEALHAGGHTFPGHTEILAHLTGTDEVSMLLMTPTLK
                     VIHVTTHIGLIDAIARIEPGLVERTIRRGHAALVRAGTSAPRIGVCGINPHAGENGLF
                     GYGEEAAKIVPAIERLVADGLAVTGPLPADTLFFRAQRGDFDLVVAMYHDQGHGPVKV
                     MGIDAGVNVTVGLPVVRTSVDHGTAFDIAGTGTAEEASLIEAIRQAVALSAHATGARE
                     AALSK"
     gene            10881..12026
                     /locus_tag="VFR00_06710"
     CDS             10881..12026
                     /locus_tag="VFR00_06710"
                     /inference="COORDINATES: protein motif:HMM:NF017532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HEU0158983.1"
                     /translation="MATSADYGREETSMQAYLRAGERRAAELGNRGPIRFDASGRLAP
                     DIVEAYWRCGFYVFEGVLRPEELADIEADVKDILDRLPREKGAALDKHGRPALAADCK
                     APTLFWSKPLGDPFGGTALANGRHPVKMFEPIAAADAPKEVVYLILGSLQFSQACLRV
                     YGHPGLLRVAAAINGEDFVPFNEALFIKAPGLGASVAWHQDGTTHWGAPDWDEGTHGF
                     NFMGQLYGSTAANGVWVVPGSHKLGKIDIKHWVAEAGTERLPEAVPIIAKPGDVAITN
                     RQALHGSFANTSKDWRVTVNMGFHRRRSVLGVEGGGVHNARGVYDDERIAERAKMIGY
                     GIDARRQRFPDEVPFVYRPHAEAGVSYAWTEASKATVKDYNLLDLSI"
     gene            12372..13763
                     /locus_tag="VFR00_06715"
     CDS             12372..13763
                     /locus_tag="VFR00_06715"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEU0158984.1"
                     /translation="MANHVTPLQGTRKTIAVARLHRRQLLAATLAGLAGTAMGFKVSP
                     VRGQAKKFAGLTLTGAAFQSTYHEYLKTYLPEFEAETGMKVDFQQQAFPVYNQRMDLE
                     LSTKGSAYDFCNLTFIYSGRWVGANWFTPLNEFVKDANKTPPDWDADDFVSGTTQHLK
                     NAKGEIFAFPWEAGGMLMAAARGDLIDKAGLKMPTTFDDLMKVCETINGKEGVAAYVT
                     DRLHHWNFIPYLMGFGGKVFRNPPDDLMPAFDTAEGAKAADYFARLLTEMSPPGVMSF
                     TDDQAMRAQLSGRANIRTNAITWLTALVKHGESKVTTTSRFALMPAGPAGNFPGSNSH
                     GFGIPAGAKNKEAAWAFITWAVSKKTIERIVKEKGYGAVCRRSVINSDYFTQSLTLNG
                     DSVAPLYLQVLELGGKTGYMKYRTVPVFPQVGDKINKAIERIASKQQKAPEALAQAKD
                     EAIADLKKAGIQL"
BASE COUNT         2244 a         4602 c         4709 g         2213 t
ORIGIN      
        1 cgcgcccgtc gccgagcgcg ccgcggccag cgtggcgtag acctgggcca ccagattggg
       61 cgccaggccg acggttggct cgtcgatcag cagcaactgg ggcgccgtca tcagctcgcg
      121 tgccaccgag agcatgcggc cctgcccgcc cgaaagcgcg ccggcgcgct ggccgcgccg
      181 ctcgcgcagc accggaaaca ggtggtagag gtgctcgagc tggcgcgcga tgcgcgcgcg
      241 gtcgcgccgg aaggtccagg cccccatcag caagttctct tcgacggtca gataagggaa
      301 gctgttgatg tcctgcgtga tgtagctgac gccggccgcc ttcagccggc tcgggccggc
      361 gccggtcaca tccgagccgg cgatcgcgac gcggccggcg cggggcgcca gcagcccgaa
      421 aatggtgcgc agcagggtcg actttcccgc gccgttggga ccgatgacca gcgtcacggc
      481 attttcggcg gcgctcagcg acagcccgga caggatttcg acgcccggca gataccccgc
      541 acacaactcg aagacctcga gcatcggctt gccggtcatg ccgcgctcct gccgagatag
      601 gcggcaatca cctccgggcg gcgcgctacc tcctcgaggg taccctccgc ggcaatgcgg
      661 ccctcgatca tgacggacac ctgcgggcag agccgccgca cgatttccat ttcgtggctg
      721 acgatcagga aggcgcgatg gtgctcgctg ttctcgcgtt cgatgagggc gatcagcgtg
      781 tccttgacga ccgggttgat gccggcgaac ggttcatcga ggacatagag cgtgacgctc
      841 ggcaccataa agccggcgca cgcctgcaac agcatgcgct ggccgcccga gagagtaccg
      901 gcgcgctcct gcgcgagagg gctgagggtc gcaagcgcca acagctcgcg cacgcgggcg
      961 agcccggcgg caagcccgcc cgggctgccg gcggccagga agggcagcac caggttgtcg
     1021 gacacactga ggctttcgaa aatgcgcgcc acctgaaagc tgcgcaccag accgtgcgcg
     1081 gcgatctggt gggcgccgag cccgctgagc tcgacgttgc ccagccacac gcgccccgcg
     1141 tcgggggtct caatgccggt gagcagattg acgagagtgg ttttgccggc gccgttcggg
     1201 ccgatcaggc cgcgaatttc gcctggcccc aggctgaggc tcaccttgtt gttggccacg
     1261 agcccgccgt agcgcttgct gaggtcttgg gcacgcagca gagcgggcat caggcctccc
     1321 gtccgcgttc gccgaagaga ccattgggtt tgaggatcaa gacgagaatg acgagcagaa
     1381 atccgtagac gttctggtag ggcgaggaga tgaaggcggc gccgaggctt tccaccagcc
     1441 ccagcagaag accgccgatc agcgctccgg ggatcgatcc gagcccaccg atgacgatga
     1501 tctcgaagcc cttgaccgtg gtgaccgcac cattggtcgg aaacaccatg aacaggggcg
     1561 cgagcagggc gccggcaaag cccgccagcg ccacgccgat ggcaaaggcg atagcgtcga
     1621 ggcgaaagac gtcgacgccc gccgtctgca cggccacgcg attctgggcg gccgcccgca
     1681 aggcaagtcc gacccaggtc tttttgatga cgagccaaaa gctgccgagc gccaagacgg
     1741 cgaagatcga ggccgccacg cgggcgccct cctgtggcag gggtccgacc caaaccacgg
     1801 ggagggtcga ttctgggcga tactggttgg ggccgcccag cgccacggcc aggttgcgca
     1861 agagcaggag caaggcgatg gtgacgacgg tggcgtagtc gtcacgccgc tgcagcggtc
     1921 gcgcgtacat cggcttgatc aagatcggct ccaggatcaa ccccaagaga aaggtgccgc
     1981 tggccgccag gatcgccgcc agccaccacc agttcgggcc cagcaaaaaa tgcacggcga
     2041 caaactggat gtagctgccg atcatgtaga actcgcccat cgcccagttg atggtcttga
     2101 cgatcgagta gatgaaggtg atgccaagcg ccatcagcgc gtagagaacg ccgaccatca
     2161 accccgacag cagggtctgg atcagaagct cggcgctgaa aatcaccggc gcctcccctc
     2221 tcaggccttg ggctcgcctg ggccgcgcgg cgcggcccaa gcgttccagc cgctcactcg
     2281 cccagaacgt agccgaccgt cgcggtggtg ggcgtttgat ccttggcgga ataggtgagc
     2341 accatcaggg gcggcgacca gttgtgccac agcgcgccct ccgcgcgcgg gaaggtgacg
     2401 ggcgtcgtgg accagccggt gaaggagcct tcttccagcg ccttgatcat ggccttgggg
     2461 tcggtcgatt tggcttgttc gatcgccgcc gcgaacacca gcgcattggc aaagccgttg
     2521 agggcggcgt agaccggcgg ctcattgaac ttggcctggt agcgcttggc gaattccttg
     2581 ccgacctcgg acagctccga cttcggggaa tagaaggcga tgaagctaac gccgacgccc
     2641 ttgtcgccgt gcagctgcca gaactgcggc cgggccggcg catcgtagga ggtcaccatg
     2701 gccgtgcccg gcagcagacc gacggtatgg gcctgggtga tcatcaggtc gagcggctgg
     2761 ccaacgccga cgttgacgat ggcatcgggc ttgaatgcct tcacctgcag gagctgcggc
     2821 aggaggtcgg tcgtggtgcg gtcgaacatg atggtctgga tctcgagacc aaggttcttg
     2881 tccttgttct gcttctcggt ttccttggcg agcccgatgc cgtagtcggt ggtttcggca
     2941 atcaggctga cccgcttcca gttgttcttt ttgatccagc cgagccaggt ggccgcccgc
     3001 ggcggatcga cggcgtgggt gcgaaaggca atgtcatagc gcttggcggt gacatcgctg
     3061 gcacccgtct gcgtccccat cgtgggcacg cccatttcct tggccacctc gttgacggcg
     3121 atgttggcgc ccgagtgaat aaagccgaac acggcgaccg ccttttcgct ggagaccagc
     3181 cggcgatagg ccgcaacggc ctgctcgttc ttgccggcgc tgtcgtgcac cgacagggcc
     3241 accttgcgcc cgagcacgcc gcccttttcg ttgatgtact cggcgccgat ctccgcgccg
     3301 cgcttggcga gctgacccaa cgccgtttcg ccgggtgggg acagcggcgt gatcacgccg
     3361 aagacgatgg gctcctgcgc acaggcctgg ccgaggcccc aaagcatcga caccagcagg
     3421 gctccgcctg caaaaccccc tcgccgcatg gctctcctcc tcgcctcaca tcctcacgcg
     3481 gcgcaagcag ggatggcgag cgccgatgcc gagccaatac tgccgtggca aacggttccg
     3541 tgtcgagggc atcgggcctc aggccgggcg cgccagcagc cgatcgcgca gctctccgaa
     3601 gtccgccgcc acggcctcga ggtcagccac agggaagccc gccgccagcg ccgggctggc
     3661 cgccgccagg cgttcgcgca agcgcccgcg aaactcggcg cgcccgtccg cgccaataaa
     3721 gaggccgttt tccgcctcga agcgtgcgtt ccaggccacg gcgtccgccc gcgccaagcc
     3781 tgccggcagg tcgagatcga gctttcccgc cgcgaccacg acgggatagc cgccgggcag
     3841 gcctgccgga ccggggacat ggcctcgcca tgcccctccc ttggccaggg ccagcatcag
     3901 tgggacgccg ctggctccgg agatatcgat cacgggctcg ggtgtgagct gcacgggcgc
     3961 aaagcgggcg aacacgtcgt caatctcgcg gccgtcgagc cagacgcggg gcgctggccc
     4021 cgtgcgttcg gcgcgcggac gccgccaggc ggcgatggcc tgatagtgac agagcagctt
     4081 gaggtcgggc gcgggttccg ccgcggcgag gctgccggca aaggcgtggg caagaatgcc
     4141 gatattgccg atgccgcaca acaccggcag tcccatgccc tggagaatgc cgttcaccac
     4201 gtcgggaaag cagcaattga tgagctggcc ctgcagcgcc agctcgcgca tcgcgcgggc
     4261 aacgcgcgcg ctcaacagcg cttggaacac cgccgttgcg ctcaacccgc cttcggcgac
     4321 cagccggccc caggcggtgc cgccggcggc gatgacggcg ccgccctgct gggaggcagc
     4381 ctgcacaacg acgcccggct cgagtttggc gagcagcgtt tccgcagccc cggcggcgcc
     4441 gaggtcgacc gcgtaggagg tgaggcgcag cggccggccg aacatatggg cgcgcgctcg
     4501 tgcggccgtc gcgatccaat cgagccgggc cgagttgcgg ccggcgaggc cgagtgcgat
     4561 cggctcggtc gcggtggcgg ccagatcgca agcaatgcgc tgggcgaagc tcccgctgcc
     4621 aaaaatgagg atgtcgcagg cgcgcgccat gtgctcttct ccaggtagag cctttgccgc
     4681 gaccgatttg accgcatttc ccggggtctg tcagcgcctc gtctcggcta gggagctcgg
     4741 gttcgagggt ggcgcatggt gcagcgtagc gccgacgtgg caatcattgg cggcggcctc
     4801 gtcggctgtt ttgccgccta ttttttgcgc cggcgcgggc agtccgtcgt ggtgatcgaa
     4861 cgcggggcgg tctgcacggc ggcgagcggc accaacttcg gcaatttgcg cctgcagggc
     4921 cgtcaccccg ccgagtttgc cctggcgctc agggcgcagg cgatctggga agagttggca
     4981 gcgctcagcg gcgaagggcg cgccattcgg gcctcgggcc acctctatct cggcctggcc
     5041 gtaagcgacc agcccaagct cgagagcatc gcgcaggagg ccaacgccgc gggcctggcc
     5101 gtcgacctgc tggatggcaa cgcggtgcgg cgccgctggc cgatggtctc agatttgatc
     5161 acgggggcct gttggtcgcg gcgcgatggc attgccgagc ccacccggat cgggccaacg
     5221 gtcgcccgcc tggcagccgc cgcgggcgca cagctcatcg aaaacacgag ggttacgcgc
     5281 atggtgccgg gcgcgggggg tttcaccgtc tcgacggacc gggatctcag cgtcagctgc
     5341 ggccgcctca tcaacgccgc gggcgcctgg ggcgccgagc tggcaggcgc ctgcggcgag
     5401 ccggtgccgc tcttcgccgc ggggccaccg ctgtttgagg tccaccccga gcatgcctgg
     5461 gatggtccct cgctcgccgc cgtcgacggc acgctgctgc tgcgtccggg tctcaccggg
     5521 cccgccgtgg ccggcacctt tccgcgcgtc aaagccgatc tcggcagcgg ccgtgcgccg
     5581 gtgccgcccg agcgcgtcgg ccccgccctg gcgcatctca acagcgtcgt gccagggctg
     5641 gggcggcttc gggctggccg cgtctggtcg ggcgtcgagg gctacctgcc ggacatgttg
     5701 cccgtgatcg gcgcgagcgg gactactcct gggctggtgc atgccttcgg cttctcgggt
     5761 catggtttcc agctggcgcc gggcgtcggt gcggtcttgg ccgagctcgt taccacgggc
     5821 gtgagcgaga ccccgattgc ggcctttgcg ctggcgcgct ttgccgacgg cgctgccgtc
     5881 gtcgacgaca agctctggcg ggaattcgat cccgagctcg tggcccgctt tcgccggggg
     5941 cacgcgggca cggcagacgc gagcaggcat gacctttaag tgtcacgcag ggcggcacgg
     6001 cgccatgcgt ttcaagggat tgaggtggag ggcacaacca atgagcgacg cgcaccccgt
     6061 tctgaccgac ctcagcgatg gtctcgccac gatcacgctc aacaagccag ccaagctcaa
     6121 cgcctgggat acgcccatgc gggcggagat cgctgccatc ctgaaggctt ggaacggcga
     6181 tgacagggtg cgggcggtga tcgtcaccgg cgctggggaa cgcgccttct cggcggggca
     6241 ggacctcgac gagaccgaaa aattcagctc tggcgatgac ggcgccaact ggtttcaatc
     6301 ctggcgtgac ttctacaacg ccattcgcga cctcgataaa ccgtgcgtgg cggcgctcaa
     6361 tggggttgcc gccggctccg cctttcaggc ggccatgctg atggacgtgc gcgtcggcca
     6421 tcgcggcgtg cgcatggggc aagccgagat caattcgggc atcccgagcg tgaccggccc
     6481 gatgttgatg ctgtcgcgta ttggcctgtc gagaaccatc gagttgacgc tgaccgggcg
     6541 catgatggag gcggaggaat gctgcgccgt cggtctcatg cataatatgg tcgagcggcg
     6601 cgaggaggtc ttgcccaagg cgcgcgaagt ggcgctgctg ctcgccgcca aacccgtcat
     6661 cgccatgcgg ctgaccaagg cccgcttccg ccaggtcacc caaccggcct ttgacgaggc
     6721 tttcgccaac ggcggcgtct accaggccga ggcgttcgcc tcgggcgagc cgcaagccgc
     6781 catgcggacc ttcttcgccg agcgggcagc ccggcgcaag gcgcgcgccg cgggctcgac
     6841 gtccccatct acgtgatgcc gcgggacagc gttcgtggcg ccggcccccg caccctttcc
     6901 cgacacggac gcaggctttg tcgggctgtt cgatgcccaa gcgcgggcaa cgccgcagcg
     6961 caccttcgcc acgcttgacg gtgaggcgct gagctttggc gccctcgccg agacctcggc
     7021 ccggctcggg gcctggctcg acgcgctcgg cgttgcagcc ggcgatcgcg tcgccttgat
     7081 gctgcgcaac ggcgaggccg cgctcagcct catgctggcg ttggcgcgga cgggcagggt
     7141 ctgggtgccg atcaatccgc aggccgtggg cgatagcctc gcttacgtcc tgcagcacgc
     7201 cgagccgcgc ctcgtcatcg ccgaagcgga gctgctgccg gccattgggg caagcggcgt
     7261 taacctcggc gaacgcctgc tcgaggtcga ggcggcgcgg tgcgcggccg caaacctgcc
     7321 cgcgaaggat ccgcggcctc tccccgccgc cgacgccccg ttcgcgatca tgtacacctc
     7381 cggcacgacc ggccggccca agggcgtaat cgtctcacac cgcatgctgc gcttgtccgg
     7441 cgaagccgcc gccctcgtcg ccgcgcccgg ccccggcgat gtcttttacg tgtgggaacc
     7501 gctctttcac atcggcggcg cccagctgct ggtgctgccg ctgatccgcg cggtgcggtt
     7561 ggcgctggcg caaggatttc gcgcgcgcag cttctgggcc gacgtccgcg ccgcacaagc
     7621 cacccacatg cattttctcg gcggcatcct gcaaatcttg ctgaagcagc ccgaggggcc
     7681 cttcgaccgc gaccatggcg tgcgcatcgc ctggggcggc ggctgtcccg ccgagatctg
     7741 ggcggccttc gaggcgcgct tcggcctcaa aatccgtgag tgctacggca tgaccgagtg
     7801 ctcgagcatc accacggcca atctcgacgg acccatcggc tcggtcggca agcccgtgcc
     7861 ctggttcgac gtaacgatcc tcgacccggc cggacagccc gttcgggccg ggatgcgcgg
     7921 cgagatggtc gtcacggagc gcatgcccgg cgctctcacg gagggctact tcagggatcg
     7981 cgaggcgacg gcgcaggcct tgcaccagag cggcgtgttc cggacgggag atctcgcctc
     8041 gtgcgacggg gacggcaatg tctacttcca cgggcgcctg accgacagcg tgcgcgtacg
     8101 cggcgagaac gtcagcgcgg ccgaggtcga gagtgtggtg cggcaacacc ccgccgtcga
     8161 agactgcgcc atcatcgcag tggcggcgga ggtgggcgaa gccgagatca aactctttgt
     8221 taccttgcgc cccgggggcc agctcggagc cgccgagctg tcggggtggt tgacggggcg
     8281 cctcgcgcgc tatcagaggc cgcgctacat cgctgtcctc gacgccttcc agcgcacgcc
     8341 gagcacgcgc attatgaagc acgttctgtc gcggcggctt gacgattgct gggacgcggg
     8401 cccggcccgt gcatgaggca aggctgaccc gcgcctgtgg cgctggtcat tcgacgtcga
     8461 ccccagccgc gcgctacaaa gggctaggat gcctgaggtc ttcatccttg ccgatgatct
     8521 ctccggcgct gccgactgcg ccggcggttg cgcggccgca ggcctggaca ctgtcgtgct
     8581 gctcgcaccg gatttggcgg cgcgagccag ggtgatcgcc atcgacctcg acacacggga
     8641 gagctgggca ggggaggcag gcctcaccat ggctcgcacc ctcgagcgcg cgtgggaccc
     8701 caacatcaaa gtcatctatc acaagatcga ttccaccttg cgcggggcct gggcgcacga
     8761 gctggccgcc gcacgtcaga gcctggccgc gatgatcggg tcggcaccgc ttgccctcat
     8821 cgcgcccgcc ttcccggcac gggggcgcac gacccgcgcc ggcagggtgc tggttgaggc
     8881 gtcgtccagc aaaccagtgg ctgtggcgag ggatgcgggg gtaatcgcgg caccgctgcg
     8941 gcagcttgga ctcgacgtgc gcagccttgc gcgcggcgag gtcaacgcca ccacgcccgc
     9001 cctagcacag cggttcgccg cgctggcgcg tgcaaaggtc gatgcggtcg tgtgcgatgc
     9061 ggaaacggag caggatctcg ccgccattgc cgccgcaggg ttggcgtcga gcctcccgct
     9121 gctgtgggtc ggctcggcgg gcttgatgcg tccgctggcg gccgcgctcg ccgacagtga
     9181 cacacgacca gtcgcgctgc cggccgtgcg gggacccctg ctattcgtcg ttggcagcgc
     9241 cgcggcagcg gcgcgcgccc aggccgaggt cctggcggcg gagccggaca tcaccgtcat
     9301 ccgccttcgt cccgaggatc tcatgcaggg gcacgcggct ctcgcggcgg cgctggaggc
     9361 cggccttgac cgcggcaggg atatcgctct cctcatcgat ccggcgagcc ctgcccatgg
     9421 gccgctcgac cctgcgctgg tggcaaggct cgccgacatc gcagggccgc ggctcgcgcg
     9481 cacgggcggg ctcgtcgcca cgggcgggga gacggcgcgt cgcctgctgg agcgggcttg
     9541 catctccagc atcaagcttg gcggggaaat ggaggtgggg atcccctggg gcatcgcttt
     9601 gggcgcgccg gcgcggcagc cgcagctgtt ggaggccgag gcctcaaacg ttataggttg
     9661 ggagccgagc tttcccatcg ttaccaaggc cggcggcttt ggcgatcccg gcacgctcgt
     9721 ccgctgccgc caggtcttga agactaagga gaggtgagcc agtgacgttg gagacgttga
     9781 ggtctgccgg ggggaacgcg tgaggccgat catcgccatc accatgggcg atgctgcggg
     9841 tatcgggccc gagatcatca tgcgggctct cgcgcacgcc gagctctacg agggctgtcg
     9901 acccctggtg gtcggcgatg cggagcggtt gcggcgcgcg ggcgggttgg tcggcagcaa
     9961 cgtgactgtc agggctctcg ccgatccgag gcaagcgcgc ttcacgccgg gcacgctcga
    10021 ctgcattgat gtcggcttga tccccgcaga cctgccgtgg gggaaagtct cgcccatcgc
    10081 gggcgatgcc gcctaccgct acatcgagcg cgccgtggcg ttggcgatgt ccaagcgcat
    10141 cgatgcgatc tgcaccgcgc cgctcaacaa ggaggcgctg cacgctggtg gtcacacatt
    10201 tccggggcac acggaaatcc tcgcgcattt gaccggcacg gacgaagtat cgatgctgct
    10261 gatgaccccc accctcaagg tcatccacgt caccacccac atcggcctga tcgatgccat
    10321 tgcccggatc gagccggggt tggtggagcg caccattcgg cgcggccatg ccgccctggt
    10381 gcgcgcgggg acctccgccc cgcgcattgg ggtgtgtggc atcaacccgc acgccggcga
    10441 gaacggcctc ttcggctatg gcgaggaggc agccaaaatc gtgccggcga ttgagcggct
    10501 cgttgccgac gggcttgcgg tgacaggccc gttgccggcg gatacgctgt tctttcgcgc
    10561 ccaacgcggc gacttcgatc tagtggtggc catgtatcac gatcaaggcc atggcccggt
    10621 gaaggtgatg ggcatcgacg cgggcgtgaa tgtcacggtg gggctgcccg tggtgcgcac
    10681 ctcggtcgac cacggcacgg ccttcgacat tgcgggcacc ggtaccgccg aggaggcaag
    10741 cctgatcgag gcgatccgtc aggccgtggc actcagcgcg catgctacgg gcgcgcgtga
    10801 ggcagccttg agcaaatgag ggttggtcaa cacgcggccc cggcatcggc ccgggcacaa
    10861 ggacacctga ggagcacggc atggcgacat cggcggacta cggtcgcgaa gaaacctcaa
    10921 tgcaggccta tctgcgcgcg ggcgagcgtc gcgcagctga gctcggcaat cgcgggccga
    10981 tccgcttcga cgccagcggc cggttggcgc ccgacatcgt cgaggcctat tggcgctgcg
    11041 gcttttacgt gttcgagggc gtgctgcggc cggaggagtt ggccgacatc gaagccgacg
    11101 ttaaggacat cctggaccgg ctcccccgtg aaaaaggcgc tgccctcgat aagcacggcc
    11161 gtcccgcgct cgccgccgac tgcaaggcgc cgacgctgtt ttggtccaag cccctcggcg
    11221 atccctttgg tggcacggca ctcgccaatg gccgccatcc cgtcaagatg ttcgagccga
    11281 tcgccgccgc ggacgcgccc aaggaggtgg tttatttgat cctgggctcg ctgcagtttt
    11341 cgcaggcgtg cctgcgcgtc tacggccacc ccggcctctt gcgcgttgcc gccgccatca
    11401 acggcgaaga cttcgtgccc ttcaacgagg cgctcttcat caaggcaccc ggcctcgggg
    11461 cctccgtcgc ctggcaccag gacggcacca cgcattgggg tgcacccgac tgggacgagg
    11521 gcacgcacgg cttcaatttt atggggcagc tttacggctc gactgctgcc aatggcgtgt
    11581 gggtggtgcc gggctcgcac aagctcggca aaatcgacat caagcactgg gtggcggagg
    11641 ctggcaccga gcggcttccg gaggccgtgc cgatcatcgc caagccgggc gacgtggcga
    11701 tcaccaaccg ccaggctctg catggctcct ttgccaacac cagcaaggac tggcgcgtca
    11761 ccgtcaacat gggcttccac cggcgacgct cggtcctggg cgtcgagggc ggtggtgtgc
    11821 acaacgcgcg cggcgtctac gacgatgagc gcattgccga acgcgccaag atgatcggct
    11881 acggcatcga cgcacgtcgg cagcgcttcc cggatgaggt cccgttcgtg tatcggccac
    11941 acgccgaggc tggggtgagc tatgcctgga ccgaggcgag caaggccacc gtcaaagact
    12001 ataacttgct cgacctcagc atctaaaagc gagggccgtg ccatctgcgc cagccgcgat
    12061 gctggcacgc gccggcccgc ggttgcactc ggacacttct ccatgtcggc gtgctccgag
    12121 ccgtaaccgt gaagccctac gcgccaattt gccgccatgc gcgatggcac agccgtggcg
    12181 cgacggggtt tgccagctgg cagtgcgtgc acacgcgctc tccataccgc tgcagacgtg
    12241 ttcgcgatag gcatactccc gcgcaatttc atattgcgtg gtccccgtgt tggcatttgg
    12301 cgagtgctgt ggcacggtcc aagccaacga agcgggcacc acgcgcgggt gtcactcagg
    12361 gaagatacgc catggcgaac cacgtcacgc ccttgcaagg gacacgcaag actatcgcgg
    12421 tagcgcgact gcatcgccgc cagctcttgg cggcaacgct ggctggcctt gcgggcacgg
    12481 ccatgggttt caaggtgtcg ccggtccgcg gtcaggcgaa aaaatttgcc ggcctcacgc
    12541 tcaccggggc ggcgtttcag agcacctatc acgaatatct caagacctac ctgcccgagt
    12601 ttgaggccga aaccggcatg aaggttgatt tccagcagca agcctttcca gtttacaacc
    12661 agcgcatgga ccttgagctc tcgaccaagg gctcggctta cgatttttgc aacctgacgt
    12721 tcatctattc cggacgctgg gtgggggcca attggttcac acccctcaat gagttcgtga
    12781 aggatgccaa caagaccccg cccgattggg atgcggacga tttcgtctcc ggcaccaccc
    12841 aacacttgaa gaatgcgaag ggcgagatct tcgccttccc ctgggaggcc ggcggcatgt
    12901 tgatggcggc cgctcgaggt gacctcatcg acaaggccgg cctcaagatg ccgaccacct
    12961 tcgacgactt gatgaaggtc tgcgagacga tcaacggcaa ggagggcgtt gcggcctatg
    13021 tgaccgaccg gttgcaccac tggaatttca tcccgtatct catgggcttc ggcggcaagg
    13081 tcttccgcaa tccgcccgac gatctgatgc ccgccttcga caccgcggaa ggcgcgaagg
    13141 ccgccgacta ttttgcgcga ctgctgaccg aaatgtcgcc gcccggggtg atgtcgttca
    13201 ccgacgatca ggccatgcgc gcccagttgt cgggacgcgc caatatccgc accaacgcca
    13261 tcacctggtt gaccgccctg gtcaagcacg gggaaagcaa ggtgaccacg acctcgcgtt
    13321 tcgcgctgat gccggccggg cccgccggca acttcccggg ctccaactcg catggcttcg
    13381 gcattccggc cggcgccaaa aacaaggagg cggcctgggc ctttatcact tgggcggtct
    13441 ccaaaaagac catcgaacgc atcgtcaagg agaagggcta cggcgcggtt tgccggcgct
    13501 cggtcattaa ctcggactac ttcacccaga gcctaaccct caacggcgac agcgttgcgc
    13561 cgctctatct gcaggtgctc gagctcggcg gcaagaccgg ctacatgaag taccgaaccg
    13621 tacccgtatt cccgcaggtg ggcgacaaga tcaataaagc gatcgagcgc attgccagca
    13681 agcagcaaaa ggcgcccgag gcgctggcac aggccaagga tgaggccatt gccgacctca
    13741 aaaaggccgg tatccagctt tgatgcgg
//
LOCUS       DASTMT010000291         2673 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_5657832, whole genome shotgun sequence.
ACCESSION   DASTMT010000291 DASTMT010000000
VERSION     DASTMT010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2673)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2673)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2673
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5657832"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..228
                     /locus_tag="VFR00_06720"
     CDS             <1..228
                     /locus_tag="VFR00_06720"
                     /inference="COORDINATES: protein motif:HMM:NF040327.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6111 family protein"
                     /protein_id="HEU0158985.1"
                     /translation="TPMAMYIAYMLLTRRAASAGEVANDAPLIWLFAAGALLVVATLV
                     FYGSTSGGRPDQHYTPPHMGKDGRIEPGQIK"
     gene            235..1491
                     /locus_tag="VFR00_06725"
     CDS             235..1491
                     /locus_tag="VFR00_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HEU0158986.1"
                     /translation="MTAAAASLPPRQLSDAAWLTRPGTRAVFAALAAKGFEARAVGGA
                     VRDALIGRPVSDVDIATVARPEEVMAAAAAAGLEAIPTGLQHGTVTVVADGHAYEVTT
                     LRQDVETYGRHAKVAFTADWAADARRRDFTLNALYCGADGALFDPLGGWPDLAQRRIR
                     FIGDAAARIREDYLRILRFFRFAADYGDGVPDREGLEAAVAERDGLALLSAERVRQEV
                     LRLIAAPRGPQMVRVMLEYGLICPVLGQAPRPLLLERVARLEAGLGLSPDPHLRLAAL
                     AVETREDAEQLRTRLRLSNEAFAALARVEAGKAAFAASMPTGKAKALRYSLGQAGYRA
                     LLLLDWARSGDPVETGEWRGWWALGEAWQPASLAVSGEDVMALGVPAGPRVGTLLRAL
                     EAWWIAGDFTADRPALLAKLRELSGA"
     gene            complement(1572..>2673)
                     /locus_tag="VFR00_06730"
     CDS             complement(1572..>2673)
                     /locus_tag="VFR00_06730"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018043019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEU0158987.1"
                     /translation="GELKEQLDHIRTLTLSRGESTWLRGNMFYGKRQMFAPDFIQWLE
                     SFRYPAYHLERIGDQYELLFEGSWIETTMWEIPALAVLMELHSRAVTKGMGKFELQVL
                     YARAMTRVWEKIGRLKLLPGLNIADFGTRRRHGFLWQDWCVQAMIEGLGGAFLGTSNC
                     LIAMRREVEAIGTNAHELPMVYCALADNDRQLAEAPYKVLADWQEDYDGNLRVMLPDT
                     YGTPGFLERAPDWVTHWTGMRIDSGDPIASGEIAVDWWRRRGQDPRLKLAIFSDGLDV
                     ESIEHIHRHFHGRMRLGYGWGTLLTNDFRGLGPDTRLDPFSIVCKVISANGRPAVKLS
                     DNALKAMGPPQEVERYRRVFGTTAPASLPVAV"
BASE COUNT          387 a          880 c          962 g          444 t
ORIGIN      
        1 acgccgatgg ccatgtacat cgcctacatg ctgctgacgc gtcgggcggc gtcggcgggg
       61 gaggtggcca acgatgcgcc gctcatctgg ctattcgccg cgggcgcgct cttggtggtg
      121 gcaaccctgg tgttctacgg ctccacctcg ggcggccggc ccgaccagca ctacacgccc
      181 ccgcacatgg gcaaggacgg gcgcatcgag cctggtcaga tcaagtaaaa ggccatgacc
      241 gccgctgccg catccttgcc accgcgccaa ctgagcgatg ccgcctggct cacgcggccc
      301 ggcacgcgcg ccgtttttgc cgcgctcgcc gccaagggct tcgaggcgcg cgcggtgggc
      361 ggcgcggtgc gcgacgcgct catcggccgg ccggtgagcg atgtcgatat cgccaccgtg
      421 gcgcgccccg aagaggtgat ggcggcggcc gcggccgcgg gcctcgaggc gatcccgacc
      481 gggctgcagc acggcaccgt cacggtcgtt gccgacgggc acgcctacga agtcacgacc
      541 ttgcgccagg acgtggaaac ctacggtcgc cacgccaagg tggccttcac cgccgattgg
      601 gcggccgatg cgcgccggcg cgatttcacg ctgaatgcgc tttattgtgg cgccgacggc
      661 gcgctgttcg atcccttggg tggctggccc gatctggctc agcgccgcat tcgcttcatc
      721 ggggatgcgg cagcgcgcat tcgcgaggac tatctgcgca tcttgcgctt cttccgcttt
      781 gccgccgact acggcgacgg cgtgcccgat cgcgaggggc tcgaggctgc cgtggcggag
      841 cgcgatgggc ttgccttgct ctcggcggag cgcgtgcgcc aggaagtctt gcgcctgatc
      901 gcggcgccgc gcgggccgca aatggtgcgc gtgatgctcg agtatggctt gatttgcccg
      961 gtgctggggc aggcgccgcg gccgctgttg ctggagcggg tggcgcggct cgaggcgggg
     1021 ctcggtctca gccccgatcc gcatttgcgg ctcgccgcct tggccgtgga aacgcgcgag
     1081 gatgccgagc agctgcgcac gcgcttgcgg ctgtcgaacg aggctttcgc ggcgttggcg
     1141 cgggtcgagg ccggcaaggc cgcctttgcg gccagcatgc ccacgggcaa ggccaaagcc
     1201 ttgcgctata gcttgggcca ggcgggctat cgcgccctct tgctgctcga ctgggcgcgc
     1261 tcgggcgatc ctgtggaaac cggcgagtgg cgcggctggt gggcgctggg ggaggcctgg
     1321 caaccagcct cactcgcggt gagcggggag gatgtgatgg cgctgggcgt gccggcggga
     1381 ccgcgcgtgg ggacgctgct gcgcgcgctc gaggcctggt ggatcgctgg cgacttcacg
     1441 gcggatcggc cggcgttgct cgccaagctg cgcgagctca gcggcgcctg agctcggcgc
     1501 gcgccctgag cgcggctggg cgatgcggca aggcgctgat ggctcggctg gcccggcaaa
     1561 gcctggcgtg cctagacggc gaccggcaag ctcgccggcg cggtcgtgcc gaacacccgc
     1621 cgatagcgct cgacttcctg cggcgggccc atggctttga gcgcgttgtc cgacagcttc
     1681 acggcggggc gcccattggc cgagatcacc ttgcagacga tcgagaaggg atcgagccgc
     1741 gtgtcgggac cgaggccgcg aaagtcgttg gtgagcagcg tgccccagcc gtagccgagc
     1801 cgcatgcgtc catggaaatg gcggtggatg tgctcgatcg attccacgtc gagcccgtct
     1861 gagaagatcg ccagtttgag gcgcgggtcc tgcccgcgcc ggcgccacca gtcgaccgcg
     1921 atttcccccg aggcgatggg atcgcccgaa tcgatgcgca tgccggtcca gtgcgtgacc
     1981 caatccggcg cccgctccag aaagccgggc gtgccgtagg tgtcgggcag catcacgcgc
     2041 aggttgccgt cgtagtcctc ctgccaatcg gccagcacct tgtagggagc ctcggcgagc
     2101 tggcggtcgt tgtcggcgag cgcacagtag accatcggca attcgtgcgc gttggtcccg
     2161 atcgcctcga cctcgcgtcg catggcgatg agacagttgg aggtgccgag aaaggcccct
     2221 cccagccctt cgatcatcgc ctgcacgcac cagtcctgcc ataaaaagcc gtgccgccgg
     2281 cgcgtgccga aatcggcgat attgagcccc ggcagcagtt tgaggcggcc gattttctcc
     2341 cagacgcgcg tcatggcgcg ggcgtagagc acctgcagct cgaacttgcc catgcccttg
     2401 gtgacggctc ggctatgcaa ctccatcagc acggcaaggg ccgggatctc ccacatggtg
     2461 gtttcgatcc aggacccctc gaacaggagc tcgtactgat cgccgatgcg ttccagatga
     2521 taggcggggt agcggaagct ctcgagccac tggatgaaat cgggcgcaaa catctggcgc
     2581 ttgccataga acatgttgcc gcgcagccaa gtggattcgc cacgcgacag cgtgagcgtg
     2641 cggatatggt cgagctgctc cttgagctct ccg
//
LOCUS       DASTMT010000292         6995 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_1796077, whole genome shotgun sequence.
ACCESSION   DASTMT010000292 DASTMT010000000
VERSION     DASTMT010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 6995)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6995)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6995
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1796077"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..222
                     /locus_tag="VFR00_06735"
     CDS             <1..222
                     /locus_tag="VFR00_06735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008131558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEU0158988.1"
                     /translation="PQIGLQFGPLIFMICVLGGLGNMIGGFIAAFIISQFIAVGSSCF
                     HTEWGYAVAFLFFMVAIFIRPQGLFGTKS"
     gene            219..1301
                     /locus_tag="VFR00_06740"
     CDS             219..1301
                     /locus_tag="VFR00_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018272974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEU0158989.1"
                     /translation="MTSLRPTPAAIVGILLIAAIPLLVRALFPASEKYYLHLLIQILL
                     WSFIYTGWSLMGRFGLTSLGHGAFTGIGAYATVLLWNYAGLTPWIGMPLGVLLATIVG
                     VLVGYPCFRLRITGHYFALLTLALTEFVRLCIVGLRDYTGGSLGTQPRRFDGGVSLYA
                     LQFEPDRVLAYYIAFALWLFGLYVWMRVDRSMDRYALDAASQDEDAAASVGIDVTREK
                     LKITALSAAICALGGAFFAQYQMYIGPDTIAGLGVSLNIVFAVIAGGLYVMLGPTVGA
                     VFTQLLAEGLRVIIQGSAGLRGLLGNSALALDQAIYGLLLVLFIIYMPKGILGTAMDW
                     WRRRRPGAAALAVAAAAGSAGKASGG"
     gene            complement(1325..3913)
                     /locus_tag="VFR00_06745"
     CDS             complement(1325..3913)
                     /locus_tag="VFR00_06745"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing tetratricopeptide repeat
                     protein"
                     /protein_id="HEU0158990.1"
                     /translation="MRLVSARRRWRFFAAALLGLALLDPARAQQASKDLADLRSTASE
                     LYKSGEFTEALRLYERATPLVVRDFGAEHEQMAIHYHSLGLVAEAAGDLAAAERYYRA
                     AIPIREKVYGPDSAGTAIALDQLAGVYLKMGRADAADPLVRRAAKIRQDVGALLGPNH
                     AFFAGDHANRGDLNLTRGDWPAALAAYREAIRLLTGQDTSQTVVQSIVEEQLKRQRET
                     FIGLCRAAWQMRSAPDANAAALFEETFAAAQSAWATSAASALAKMSARLGAGSTPLGQ
                     RIRRVQDLSDRVLRLNAEDNTLLADWSAVQRQDARYSAAQEEFRAASLARGKATAPTV
                     KRQTELVQQLTALMQRCPPGQKRAGCEGSEAEIKAIGQQLAELSKASASGSDEIMAIH
                     RRLEAAEKALPGYAAFSTRRTALRGELDKSEDEVRQVRAEIVKSFPQYAALTDPKPLL
                     VQDVRALLRDDEALVAMLVGSSHSFVWALTRERLAWAEIKAGAKELAEHVTRLRSGLD
                     PLAQQDAEGSPTSRAGIVQGFDLERAHRLYRLLLGPVAEVVNAKHHLIIVPTGPLTSL
                     PLQVLVKEAPTLGATPQETFRRVPWLIRSHALSVLPSVQSLSALRRLAPTGAASKPFF
                     GVGDPILRGPLDRQQRSAQLKLAQPARFYRNGLADTRAVRELPPLPDTADEVRTIAKV
                     LGATPDAVNLREAASETRVKSAALREYHIIHFATHGLVAGDLSGLSEPALVLTPPERP
                     SEADDGLLTASEIAALELDADWVVLSACNTAAGAGEGAEALSGLARAFFYAGARALLV
                     SHWSVFSTAATQLTTKTFATLAADPRLGRAEAFRRSMLALIEEGQGPSYWAPFVIVGE
                     AGAAAR"
     gene            complement(3945..4304)
                     /locus_tag="VFR00_06750"
     CDS             complement(3945..4304)
                     /locus_tag="VFR00_06750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011441676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
                     /protein_id="HEU0158991.1"
                     /translation="MFTGQAIFAVPLVLFYLATAVGARAQDTAAGAGVPGVVTGGSST
                     TSIGGQAAARKGDATDQGGAIVEGSKNVFIDGRPAAVLGDKTNCGGITIGGAGNVFIN
                     GKPAARAGDLTTGCAGK"
     gene            complement(4493..4990)
                     /locus_tag="VFR00_06755"
     CDS             complement(4493..4990)
                     /locus_tag="VFR00_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013420614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HEU0158992.1"
                     /translation="MAFEIERKFLVRSQEWRTLATAQTRVRQAYLAGGDKSSTRVRIK
                     DDVAASLTIKARGVGLRRLEVEVPISLIDGEALLSLRQSSLIEKIRHEVPWQRKTWEI
                     DVFAGDNAGLIVAEIELGDEREVFERPPWLGPEVTGQTRYYNSALAQRPYATWQKTAG
                     LLGTG"
     gene            5253..6161
                     /locus_tag="VFR00_06760"
     CDS             5253..6161
                     /locus_tag="VFR00_06760"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEU0158993.1"
                     /translation="MVFRVAQISDAHLSASKPYFVANFLRVADHIGANGADLVISSGD
                     MSLDGAAQKEDLIAAKRLHARLDLPLRYLAGNHDIGESQDTPEPRGLPLLSGLTRARY
                     VAHFGPDYWSLRVPGWRLLAVNDFLLGSDLPEAEALRRFVRQAAATAAGEALALFTHR
                     PLFHLAADEQDITGRFINPGPRAELFAALGGIWPALAACGHVHQFVSNVRAGCQHVWA
                     PSTGFVMPDTQPCYGLKRTGYVEHLFAPDGTHLSRLVPVPGLASPSITEFPQAYAQYA
                     EAGGPRGAAPEAAARKGIAPKGAAQP"
     gene            complement(6177..>6995)
                     /gene="asd"
                     /locus_tag="VFR00_06765"
     CDS             complement(6177..>6995)
                     /gene="asd"
                     /locus_tag="VFR00_06765"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015684228.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HEU0158994.1"
                     /translation="NFVLTNRIPRRALTRFMGWFGKIELPLIRGGSIAIFRLFCAPNL
                     AEAKKRSFDSLHDCFIRELKEGARRIEPAANILVSPCDGIVGAAGPIRDDTLLQVKGS
                     PYTLQELLRDRELAALYRHGNYVTLRLTAGMYHRFHAPFDGRAERVTYIPGDAWNVNP
                     PALKRIDRIYCRNERAVLSLRLAGEQRPLALVAVAAVLVAGIRLRCLDLPFERHGRAP
                     TVLACAAQFAKGDELGWFEHGSTILVLAPAGFRLCANVSDGALIQMGQPLLLHP"
BASE COUNT         1073 a         2367 c         2306 g         1249 t
ORIGIN      
        1 ccgcagatcg gcctgcagtt cggaccgctg atcttcatga tctgcgtgct gggcggcctc
       61 ggcaacatga tcggcggttt cattgcggcc ttcatcatca gccaattcat cgccgtgggc
      121 tccagctgct ttcacacgga atggggatac gccgtggctt ttctgttttt catggtggcg
      181 attttcattc gtccgcaggg tttgttcggg actaaatcat gacgagcttg cgccccacgc
      241 ccgccgccat cgtcggcatc ctcttaattg ctgcgatccc cctcctcgtg cgcgcgctct
      301 ttccggcctc ggagaaatat tacctgcacc tgttgatcca aatcctgctg tggtccttca
      361 tctatacggg atggtcgcta atgggccgct tcggcctgac ctcgctcggc cacggcgcct
      421 ttacggggat cggtgcctac gccacggtcc tgctctggaa ctacgccggc ctcacaccct
      481 ggatcggcat gccgctgggc gtgctgttgg ccacgatcgt cggcgtcctc gtcggctatc
      541 cgtgctttcg cctccgcatc accgggcact atttcgcgct tttgaccctg gcgctcaccg
      601 aattcgtgcg cttgtgcatt gtcggtctgc gtgactacac cggcgggtcg ctcggcacgc
      661 aaccgaggcg gttcgacggc ggcgtttcgc tgtatgccct gcaattcgag cccgatcgcg
      721 tgctcgccta ctacatcgcc ttcgcgctct ggctgttcgg gctctacgtt tggatgcgcg
      781 tcgaccgcag catggatcgc tacgcgctcg atgccgccag tcaggatgaa gatgctgccg
      841 cctcggtggg catcgatgtg acccgtgaaa agctcaagat cacggcgctg tcggcggcca
      901 tctgcgccct gggcggtgcc ttcttcgcgc aatatcaaat gtacatcggg cccgatacca
      961 ttgccgggct cggtgtttcc ctcaatatcg tgttcgccgt catcgccggc gggctctacg
     1021 tcatgctcgg gccgacggtc ggtgccgtgt tcacgcagct gttggcggaa ggcctgcgcg
     1081 tcatcatcca aggctcggcc ggcttgcgcg gcttgctcgg caattccgcc ctggccctgg
     1141 accaggccat ctacggcctg ctgctcgttc tctttatcat ttatatgccg aagggcatcc
     1201 tcggcaccgc catggactgg tggcgccgac gccgccccgg cgctgcggcg ctcgccgttg
     1261 ccgccgcggc gggcagcgcc ggcaaagcca gcggcggctg aggctggctc gttgctgccg
     1321 cggctcagcg cgccgcggct cccgcctcgc cgacgatcac aaagggggcc cagtaggacg
     1381 ggccctgtcc ctcctcaatc agggccagca tcgagcgccg aaaggcctcg gcccgaccaa
     1441 ggcgcgggtc ggccgcgagc gtcgcgaagg ttttggtcgt cagctgcgtc gccgccgtcg
     1501 aaaagaccga ccaatgtgag acaagcagcg cccgcgcgcc ggcatagaaa aaggcgcgcg
     1561 ccaggccgga gagggcttcg gcaccctcgc cggcgccggc ggcggtgttg caggcggaga
     1621 gcaccaccca atcggcgtcg agctcaaggg cagcgatctc cgaggcggtg agcaggccat
     1681 cgtcggcctc gctgggacgc tcgggtggcg tcagcaccag cgccggctcg ctgagaccgg
     1741 aaagatcgcc cgccaccagg ccatgcgtgg cgaaatggat gatgtggtac tcgcgcaagg
     1801 cggcgctctt gacccgggtt tccgaggcag cctcgcgcag attgacggcg tcgggggtcg
     1861 ccccgagcac cttggcgatc gtgcgcacct catcggccgt gtcgggcagc ggcggcaact
     1921 cgcgcaccgc ccgcgtgtcg gcgaggccgt tgcggtagaa gcgggcgggc tgggcgagtt
     1981 tgagttgcgc gctgcgttgc tgccggtcga gcggcccgcg cagaatggga tctcccacgc
     2041 caaagaacgg cttgcttgcc gcccccgtcg gggccagccg gcgcagggcg ctgagcgact
     2101 gcacggacgg cagcacgctc agcgcgtggc tgcggatcaa ccaggggaca cggcgaaatg
     2161 tctcctgtgg cgtggcgccc aaggtcggcg cctctttgac gaggacctgc agaggcaagc
     2221 tcgtcaaagg ccccgtcggc acgataatca agtggtgttt agcattcaca acctccgcca
     2281 cgggtccgag caggagccgg tagagccgat gcgcccgctc caaatcaaag ccttgcacga
     2341 tccccgcccg gctcgtgggc gagccctccg catcttgctg cgccagcgga tcaaggccgc
     2401 ttctgagccg agtgacgtgc tcggccaatt ccttggctcc ggccttaatc tcggcccagg
     2461 ccaggcgctc gcgcgtcagc gcccacacga agctatgcga ggaccccacg agcatggcca
     2521 ccagcgcctc gtcgtcccgc agcagtgcgc gcacatcttg gaccagcagc ggcttgggat
     2581 cggtcagcgc ggcgtactgg ggaaacgatt tgacgatctc ggcgcgcacc tggcgcactt
     2641 catcctcgct cttatcgagc tcgccgcgca gtgctgtgcg ccgcgtgctg aaggccgcgt
     2701 agccgggtaa cgccttctcc gccgcctcca ggcgccgatg gatcgccatg atttcgtccg
     2761 agccgctagc agacgctttc gacagctccg cgagctgttg gccgatggcc ttgatttccg
     2821 cctcgctgcc ctcgcagccg gctcgcttct gcccgggcgg gcagcgttgc atcaaggctg
     2881 tgagctgttg caccaactcc gtctggcgtt tcaccgtcgg cgccgtcgcc ttgccgcgcg
     2941 ctaggctggc ggcgcggaac tcttcctgcg cggcgctgta gcgcgcatcc tggcgttgca
     3001 cggcgctcca gtcggcgagc agcgtgttgt cctcggcatt gaggcgcagg acgcggtcgg
     3061 aaagatcctg cacgcgccgg atgcgctggc ccagcggagt gctgccggca cccaggcgtg
     3121 cgctcatttt ggccaacgcc gatgcggccg aggtcgccca ggcgctttgc gcggcggcaa
     3181 aggtctcctc gaacaaggcc gcggcattgg cgtccggcgc cgatcgcatc tgccaggccg
     3241 cgcgacagag gccgatgaag gtctcgcgct gccgcttcaa ctgctcctca acgatggatt
     3301 gcacgaccgt ctgggaggta tcctgtccgg tcagcaggcg gatagcctcg cgataggcgg
     3361 ccagcgccgc gggccaatcg ccgcgcgtca ggttgagatc gccccggttg gcatggtcgc
     3421 cggcgaagaa agcgtggttg ggcccgagca gcgcccccac gtcctggcgg attttggcgg
     3481 cccgccgcac gagcgggtcg gccgcgtcgg ctcggcccat cttgaggtag acgccggcga
     3541 gctggtccag ggcgatggcg gtcccggcgc tgtcgggacc ataaaccttc tcccggatcg
     3601 ggatggcggc acggtaatag cgctcggcgg ccgcaagatc cccggcggcc tcggccacga
     3661 gccccaggga atggtagtgg atcgccatct gctcgtgctc ggcgccaaaa tcgcgcacca
     3721 ccagcggcgt ggcccgctca tagaggcgta gggcctccgt gaattcgccg ctcttgtaga
     3781 gctcactcgc cgtgctgcgc agatcggcaa ggtctttgga ggcctgttgt gcccgcgccg
     3841 gatcgagcaa ggcgagcccc aacagcgctg cagcaaaaaa gcgccagcgg cgccgagctg
     3901 acacgagcct catcgccttt cccccgggat tgccaaaggt cctgctattt gcctgcgcac
     3961 cccgtcgtta gatcgccggc gcgcgcggcc ggcttgccgt tgataaagac gttgccggcg
     4021 ccaccgatgg tgatgccgcc gcagttggtc ttgtcgccga ggaccgcagc cggacggcca
     4081 tcgataaaga catttttgga accctcgacg atcgcgccgc cctgatccgt ggcgtctccc
     4141 ttgcgcgccg cggcttggcc gccaatggag gtggtcgagc tgccaccggt gacgaccccc
     4201 ggcacgccag cgcccgcagc ggtgtcctgc gcccgggcgc caacggcggt tgcaaggtaa
     4261 aacagcacca acggcacggc aaaaatcgct tggcctgtga acatccaatc cctccgcaag
     4321 gtctgccgtc gctcggcgct gccctttacg cccgtctcgc tggcgccgag cctcgcgcac
     4381 ggtcgcgcgg atcggcgcgg tgcgcaagca cctcgatgcg aagtccctcg cggcgttcgg
     4441 cccgtgccgc aacctttggc cgaaggcgcg cgtgctcccc gcgctttcgg cttcagccgg
     4501 tgccgaggag gccggccgtc ttctgccagg tcgcgtaggg gcgctgcgcc agggcgctgt
     4561 tgtaatagcg cgtctgaccc gtcacctccg ggcccagcca gggtggacgt tcgaagacct
     4621 cgcgctcgtc gcctagctcg atttccgcaa caatcaggcc ggcattgtca ccggcgaaga
     4681 cgtcgatctc ccaggtcttg cgttgccagg ggacctcgtg gcgaatcttc tcgatcaggc
     4741 ttgactggcg cagcgacagc aacgcttccc cgtcgatgag cgaaattggg acttcgacct
     4801 cgagccggcg cagcccgacg ccccttgcct tgatggtaag gctggccgct acgtcgtcct
     4861 tgatccgcac gcgcgtcgag gacttgtcgc cgccagccag gtaggcttgg cgcacgcgcg
     4921 tttgcgccgt cgccaacgtg cgccattcct ggctgcgcac caggaatttg cgctcaatct
     4981 cgaaagccat ggatccccgc cccgctaccg ccctacgccg ccattctacc aggctctggt
     5041 ggggcattgt cactagggcg ggcttcggcg atctgccgca ggcgccgggt gagccttaag
     5101 tccttcaatg ccaagaggga gctggcgtgc gccgcggacg tgccgcctct cgcttagcgt
     5161 cgctgacgcc tgtgccccga tgatgtgggc gcccgcggct ggcctcggcg ccttcaaata
     5221 cgtgtcatgt tgtcccgtta agtcttgcgc ggatggtttt ccgcgtcgcc cagatttccg
     5281 atgcacacct gagcgccagc aagccctatt tcgtggcgaa tttcctcagg gttgccgatc
     5341 atatcggcgc caatggcgcc gacctcgtca tcagcagcgg cgacatgtcg ctcgacggcg
     5401 cggctcagaa agaggacttg atcgccgcaa agcgcctgca cgccaggctc gacctgccgc
     5461 tgcgctatct ggcggggaac cacgacatcg gtgaaagcca ggacacgccg gaacctcgag
     5521 gtttgccgct gctgtcgggg ctcacacgcg cgcgttatgt cgcccacttc ggcccggact
     5581 actggtcctt gcgcgtgccg ggctggcgcc tcctggccgt caacgatttc ctgctgggca
     5641 gcgacctgcc ggaggctgag gcgctgcgcc gtttcgtccg ccaggccgca gccacggccg
     5701 ctggcgaagc gcttgcgctc ttcacgcaca gaccgctgtt ccaccttgcc gccgacgagc
     5761 aagacatcac gggccgcttc atcaatcctg ggccacgtgc ggagctcttc gccgcgctcg
     5821 gggggatctg gccggcgctg gcggcttgcg ggcatgtgca ccagttcgtc tcgaacgttc
     5881 gcgccggctg tcaacacgtc tgggcaccat ccacgggctt cgtcatgccc gatacgcagc
     5941 cctgctacgg cttgaaacga acggggtatg tggaacacct atttgcgccg gatggcacgc
     6001 acctctctag gctcgtcccg gtgccgggcc ttgcctcgcc cagcatcacc gagttcccgc
     6061 aagcctacgc ccaatatgcc gaagcgggcg gccccagagg cgctgcgccc gaggctgcag
     6121 cgcggaaagg tatagcgcct aagggtgcag cgcagccata agcctgcagc agggttctaa
     6181 gggtgcagca acaggggttg ccccatctgg atcagcgcgc cgtcgctgac gttggcacac
     6241 aggcgaaagc cggccggggc gaggacgaga atggttgagc cgtgctcgaa ccagcccaac
     6301 tcgtcgcctt tggcaaactg agccgcgcag gccagtacgg tcggggcgcg gccatggcgc
     6361 tcgaaaggca gatcgaggca gcgcaggcga atgcccgcga ccaggaccgc cgccacggca
     6421 acgagggcga gcgggcgctg ctcgccggcg agccgcaggc tcagcaccgc gcgctcgttc
     6481 ctgcagtaga tcctgtcgat gcgcttgagc gcgggcggat tgacgttcca ggcatcgccg
     6541 gggatgtagg tgacccgttc ggcgcggccg tcgaaggggg cgtgaaagcg atggtacatg
     6601 cccgcggtca ggcggagggt gacataattg ccgtgccggt agagggccgc aagctcccga
     6661 tcgcgcagca gctcctgcaa ggtgtagggc gaacctttta cctgcaacag cgtatcgtcg
     6721 cgaatggggc ccgccgcgcc gacgatgccg tcgcaggggc tcaccaggat attggccgca
     6781 ggctcgatgc ggcgtgcccc ctctttgagt tcgcggatga agcaatcgtg caggctgtcg
     6841 aaggagcgtt ttttcgcctc ggcaaggttg ggcgcgcaga ataggcggaa gatggcgatc
     6901 gagccgccgc ggatcagcgg cagctcgatc ttgccgaacc aacccataaa tcgggtcagc
     6961 gcgcggcgcg gaatgcggtt ggtgaggacg aagtt
//
LOCUS       DASTMT010000293         3398 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_1469485, whole genome shotgun sequence.
ACCESSION   DASTMT010000293 DASTMT010000000
VERSION     DASTMT010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3398)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3398)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3398
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1469485"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..642)
                     /locus_tag="VFR00_06770"
     CDS             complement(<1..642)
                     /locus_tag="VFR00_06770"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0158995.1"
                     /translation="MIPGRWSVLALLFAVRATMGFQYQSVAAVAPLIARDMGLTLADI
                     GLLFGLYLAPGMALAYPGGSVGRRFGDRPVVLAGLALMVVGGAIMAFVPTWSGQIVGR
                     LVAGVGGVLINVLMSKMVTDWFAGRDIATAMAVFVNSWPVGIAAALVVLPPIAEMQGL
                     KAAYLFSALLVLLALVLLAQRYTPPPRSSEAATLENAMPERLALKAAIVAGSIW"
     gene            869..2506
                     /locus_tag="VFR00_06775"
     CDS             869..2506
                     /locus_tag="VFR00_06775"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HEU0158996.1"
                     /translation="MPEASETAERSAPAPVTTMELDAVARRAERLYEQGHLEAARQVA
                     LEVATRRPDHLQALLTLAHVLREEGAFEDSLGVLRRIGELSPGHVNAAAAYALTLFFK
                     EDWPQAWQAFDIRFKFPDVVPSVTLTRKDGSTYAAQPWAAGPPPASLLVMGEQGLGDC
                     IQFARFLPQLAATGSKVTCVMPRPLLRLFKTLACDVSFQPLQEGGGRVSDIGGWTPLL
                     HLPRALGITPENFVPRMPYLAAEPERVAYWRARLGSHGVRIGIVWQGNPDPRVDQGRS
                     APLSAFAPLAEIPDVRLISLQKGPRQSEIAGVAFAGAIEELGAEFDAGPDAFLDTAAV
                     MESLDLVVSVDTSVAHLAGALNRPVRILLKRLGAHWAWLYGRTDSVFYPSARLYRQRL
                     PGDWGELLQRLADDIKTQLRPPPAAELAAKRPLIPVAIGELLDKLSILRIKRARMTEP
                     QKLSNVAREQAALSAATAALTLEAGVGELAAELQAVNERLWDIESEIRACERAGDFGP
                     RFIALARAVYSNNDERARLKAEINRRTGSELIEEKAY"
     gene            complement(2594..3394)
                     /locus_tag="VFR00_06780"
     CDS             complement(2594..3394)
                     /locus_tag="VFR00_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015688820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HEU0158997.1"
                     /translation="MQLTPQQLKEFNELGYLVLPDCFSAEEMAALCAEAEAIFDSDRQ
                     EVWREKSGAPRTAFAAHTYNKAFAILGRHPRLIKPLEQFFGEPVYMHQFKINAKAAFD
                     GEVWQWHQDYGTWKRDDGMPEARAMNISVFLDEVMPYNGPLMFIPKSHKRGTLEAGHD
                     KATTSYPLWTLDKAMVTKLVADGGIVAPTGKPGTVLMFHGNLAHASPPNITPYPRKIV
                     YLTLCAVSNHITKFNRPEFIAHRDFAPIEPCADDELLTFARKYRQAAE"
BASE COUNT          600 a         1137 c         1105 g          556 t
ORIGIN      
        1 ccagatgctg ccggcgacga ttgcggcctt gagcgcgagg cgctcgggca tcgcattttc
       61 gagcgtggca gcctcgctcg agcgcggtgg gggggtataa cgctgggcca gcagcaccag
      121 agccaaaagc acaagcagag ccgaaaacag gtaggccgcc ttcaagccct gcatttccgc
      181 aatgggcggc aagaccacga gggcggcggc gatcccgacc ggccaggagt tgacgaacac
      241 cgccatggcc gtggcgatgt cgcggcccgc gaaccagtcg gtcaccattt ttgacatcaa
      301 cacgttgatg agcacgccgc caacgccggc caccagacgc cccacgattt ggccgctcca
      361 ggtgggcaca aaggccataa tggcgccccc caccaccatc aaggccaggc ccgcgagcac
      421 caccggccgg tctccaaagc gccgcccaac gctgccgccc gggtaggcga gcgccatgcc
      481 cggcgccagg tagaggccga acagcaggcc gatatcggct agcgtcagcc ccatatcgcg
      541 cgcaatcagc ggcgcgaccg cggccacgct ctggtactgg aagcccatgg tcgccctaac
      601 ggcgaacagg agtgccagca cgctccagcg cccggggatc aatggataac tccgccctca
      661 aggtggatcg cttgcattct cggtaacaca aacttgcgag tcgagaccaa tttggatttt
      721 ctcatgtggc cagagcatgg cgcccgaggt gtcgctacca catcgataaa aagagcttct
      781 atgcccgcca cgcccgcgag cctcgtgaca gggtcggccg ctaaccggcc ggtcgcaatc
      841 cctaggtcat ttcacccgag taaagcacat gcccgaggct agcgaaactg ccgagcgcag
      901 cgcgcccgcg cccgtcacga cgatggaact cgacgcggtg gcgcggcgcg cggaacggct
      961 ctacgagcaa ggtcacctcg aggcggcgcg ccaggtggcg ctcgaggtgg caacacgccg
     1021 acccgatcat ctccaagccc tgctcacgct cgcgcacgtc ttgcgcgaag agggagcctt
     1081 cgaggattcc cttggcgtac tgcggcggat cggcgagctc tcgcccgggc acgtcaatgc
     1141 cgccgcggct tacgccttga ccttgttctt caaggaagat tggccgcagg cgtggcaggc
     1201 cttcgacatc cgcttcaagt ttcccgacgt cgtgcccagc gtcacgctca cgcgcaagga
     1261 cggcagcacc tacgcggctc aaccttgggc cgcagggccg cctcccgcat cgctcctggt
     1321 gatgggcgaa caaggcctgg gggattgcat ccaattcgcc cgcttcctgc cgcaactcgc
     1381 cgccaccggc agcaaggtga cctgcgtcat gccgcggccc ttgctgcggc tctttaagac
     1441 gctggcgtgc gacgtcagct ttcagccgct gcaggagggc ggcggccgag tgagcgatat
     1501 tggcggctgg acgccgctgc tccacctgcc gcgcgcgctc ggcatcacgc ctgagaattt
     1561 cgtgccgcgg atgccctacc ttgccgccga accggagcgc gtcgcctact ggcgggcgcg
     1621 gctcggctcg cacggcgtgc gcatcggcat cgtttggcag ggcaaccccg acccgcgcgt
     1681 cgaccaaggg cgctcggcgc cgctctcggc gtttgccccg ctcgccgaaa tccccgacgt
     1741 gcgcttgatc agcttgcaga agggcccgcg gcaaagcgaa atcgccggcg tcgccttcgc
     1801 gggcgccatc gaggagcttg gcgcagagtt tgacgccggg ccggatgcct tcctcgacac
     1861 ggcggccgtg atggagagcc tcgacttggt ggtgagcgtg gatacctccg tggcgcacct
     1921 tgccggcgcg ctcaatcggc cggtccgcat cctgctgaag cggttggggg cgcattgggc
     1981 ctggctctac ggcaggacgg attcggtgtt ttatccgagc gcccggctct atcgacagcg
     2041 cctgcccggc gattggggcg agctcttgca gcgcctggcg gacgacatca aaacgcaact
     2101 gcggccgccg ccggcagccg agctcgccgc caagcgtccg ttaattcccg tcgcgatcgg
     2161 cgagctcttg gacaagctca gcattttgag gatcaaacgt gcgcgcatga ccgagcccca
     2221 gaagctcagc aacgtggccc gcgagcaggc cgcgctcagc gccgcgaccg ctgccctcac
     2281 gctcgaggcc ggggttggcg agctcgccgc cgagctgcaa gccgtcaacg aacggctgtg
     2341 ggatatcgag agcgaaatcc gcgcctgcga gcgggccggc gatttcggcc ctcgctttat
     2401 cgccttagcg cgcgcggtct acagcaacaa cgatgagcgc gcgcggctca aggccgagat
     2461 caaccgccgc accggctcag agctgatcga ggagaaggct tattgagccc ccatcgcggc
     2521 accaccccgg caggcgcaaa gacctgacgc cggccgcggc cggcaaggcc cggcggctgg
     2581 cgccccgtgc cgcttactcc gccgcctgcc gatatttgcg ggcaaatgtc agaagctcat
     2641 cgtccgcaca cggctcgatt ggcgcaaagt cgcggtgagc gatgaactcg gggcggttga
     2701 atttggtgat gtggttggag accgcacaca gcgtcaggta cacgattttg cgggggtagg
     2761 gcgtgatgtt gggcggggag gcatgcgcca gattgccgtg gaacatgagc accgtccctg
     2821 gtttgccggt cggggcgacg atgccaccat ccgccacgag tttggtcacc atcgccttat
     2881 cgagggtcca gagcggatag ctggtcgtcg ccttgtcgtg accagcctcc agcgtcccac
     2941 gcttgtggct cttgggaatg aacatcagcg gtccattgta gggcatgact tcgtcgagaa
     3001 agaccgagat gttcatggca cgcgcctcgg gcatgccgtc gtcgcgtttc caggtgccgt
     3061 agtcctggtg ccactgccag acctccccgt cgaaagccgc cttcgcgttg attttgaact
     3121 ggtgcatata gacgggctcg ccgaagaatt gctcgagcgg cttgatcagc cgcgggtggc
     3181 gaccgagaat cgcaaaggcc ttgttgtagg tatgggcggc aaaggcggtg cgcggcgcgc
     3241 ccgatttctc gcgccagacc tcctgccggt cgctatcgaa aatggcctct gcctcggcgc
     3301 acagggcggc catctcctcc gccgaaaagc aatcaggcag cacgagatag ccgagctcgt
     3361 tgaactcctt gagctgctgg ggtgtgagct gcatgacc
//
LOCUS       DASTMT010000294         1511 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_2453114, whole genome shotgun sequence.
ACCESSION   DASTMT010000294 DASTMT010000000
VERSION     DASTMT010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 1511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1511
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2453114"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..293)
                     /locus_tag="VFR00_06785"
     CDS             complement(<1..293)
                     /locus_tag="VFR00_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEU0158998.1"
                     /translation="MLARERLGLYPSIEPYRQQHLKVDAEHEIFFEECGNPRGKPVVI
                     VHGGPGGGCNASMRRYHEAARYRIILFDQRGCGRSVPHASLERNTTWDLVADM"
     gene            complement(406..>1511)
                     /locus_tag="VFR00_06790"
     CDS             complement(406..>1511)
                     /locus_tag="VFR00_06790"
                     /inference="COORDINATES: protein motif:HMM:NF012686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HEU0158999.1"
                     /translation="PQMTHGNTLRVLTRMELLRNVPIVIAPPLALDLAGVGMEALAKR
                     FVQSFGNVGTIGFVLASLVVAAGTAASPVLLPRSGTTPGVYEARKSLGWAVLVTGVVL
                     LTLPAVAVYLRFLLFEQVVGQPPEQLPAWMQILQQAGIAQIEPGPQPVSLGRFGFERD
                     AVLFALPIAAGYPQVLVYLALLGALAAALAGLSASLFALAAIVAEDVMRASPVEVASD
                     RARLGRARLALAALAFVAIWLAIALPVDPLQLFLWAATLSASTCFPVLVLAIWWRRTK
                     ALGAMAGMLSGLFVAGAGILLSETGAIALPSALAGAIGLPCAMLVTIVGSLMAPAAGH
                     NVLEQLREMRMPGGETVYDREMRLSRLKNRNPS"
BASE COUNT          301 a          518 c          489 g          203 t
ORIGIN      
        1 tccatgtcgg cgaccaagtc ccaggtggtg ttccgctcca ggctggcgtg cggtaccgag
       61 cgcccgcacc cgcgctggtc gaacaagata atgcgatagc gagcggcctc atgatagcgg
      121 cgcatgctgg cgttacagcc cccaccgggg ccgccatgga cgatgaccac cggcttgccg
      181 cgcgggttgc cgcattcctc gaaaaaaatc tcgtgctccg catcgacctt gagatgctgc
      241 tggcgatagg gctcgatgga aggataaagg ccgagccgct cacgtgccaa catcacgggt
      301 cgtttcctct gtcgctatga gctccggcaa cgccgccgaa ttccctgcag tatgggcgag
      361 cgcgcgatga aggcaagttc ccggcgctgc gcgccagcca tgtcgtcagg aagggttgcg
      421 gtttttgagg cgcgacaggc gcatttcccg atcgtagacc gtctcccctc ccggcatccg
      481 catctcgcgc aactgttcga gcacattgtg ccccgctgcc ggagccataa gactgccgac
      541 gatggtgacg agcatggcac agggcaggcc aatggcgccg gcaagggcgc tcggcaaggc
      601 aatggcgccg gtttccgaca gcaggatgcc cgcgccggct acaaataggc cgctcagcat
      661 gccagccatg gcgcccaagg ccttggtgcg ccgccaccaa atggccaaca ccaataccgg
      721 aaagcacgtc gatgcgctca gcgtggcggc ccacaggaag agttgcagcg gatcgaccgg
      781 tagcgcaatc gccagccaaa tcgcgacaaa ggccagcgca gccaaggcca ggcgggcacg
      841 gcccagccgg gcgcggtccg aggcaacctc taccggcgag gcgcgcatca cgtcctcggc
      901 cacaatcgcc gccagtgcga acaatgacgc cgacagcccg gccagggcgg ccgcaagggc
      961 gccaagcaag gccaggtaga cgagcacctg gggatacccc gccgcaatcg gcagggcaaa
     1021 gagcacggca tcgcgctcga agccgaagcg gcccaggctt acgggctgcg ggccgggctc
     1081 gatctgggca atgccggcct gctgcaagat ctgcatccac gccggcaatt gttccggtgg
     1141 ctggccgacc acctgctcga acaacaggaa gcgcaggtaa accgcgaccg cgggcagggt
     1201 cagcagcacg accccggtga cgagcacggc ccaacccagc gacttcctcg cctcgtagac
     1261 gcccggcgtg gtgcccgagc gcggcagcag cacgggcgag gcggcagtcc ccgccgccac
     1321 gaccagggat gcgagcacga agccgatggt gccgacgttt ccaaacgact gcacaaaccg
     1381 cttggccaac gcctccatac ccacccccgc caggtcgagc gccagtgggg gggcaatcac
     1441 aatcgggacg ttgcgcagca gctccatccg cgtcagcacc cgaagggtgt tgccgtgcgt
     1501 catttgcggc a
//
LOCUS       DASTMT010000295         1567 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_489421, whole genome shotgun sequence.
ACCESSION   DASTMT010000295 DASTMT010000000
VERSION     DASTMT010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 1567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1567)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1567
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_489421"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..186)
                     /locus_tag="VFR00_06795"
     CDS             complement(<1..186)
                     /locus_tag="VFR00_06795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012563235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HEU0159000.1"
                     /translation="MSDRVAAVVVAAGRGLRAGGDLPKQYRRLAGQPVIFPSLSMLAR
                     HPAISAVQPVIHADDRRL"
     gene            378..1418
                     /gene="dusB"
                     /locus_tag="VFR00_06800"
     CDS             378..1418
                     /gene="dusB"
                     /locus_tag="VFR00_06800"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011510330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEU0159001.1"
                     /translation="MPHCQEKFRSDGLEIGGIRLPNRVALAPMSGVTDAPFRRMTARL
                     GAGLVVSEMTASQDLVEGCPRTRLRAERAGVGIHVVQLAGCEPHWMAEGARMAESEGA
                     DIVDINMGCPARHVTGGQAGSALMRDLDHALTLIEATIAAVSVPVTLKMRLGWDERSI
                     NAPELARRAEAAGVRLITVHGRTRCQFYKGRADWGVIRAVVDSVAIPVLVNGDIETFD
                     DVDAALAMSGADAVMLGRAVQGRPWLPGQVARYLASGQRQGPPPLAIQFDIVNELYEA
                     MLTHHGLAVGRRHARKHLGWALDAAAHSTGAPADRLKSFRQRVLTEDDPCAVRRHLAD
                     AFDAMDSARAAA"
BASE COUNT          261 a          530 c          515 g          261 t
ORIGIN      
        1 gagccgacgg tcatcggcat ggatcaccgg ctgcaccgcg gaaatcgcag ggtgccgggc
       61 cagcatcgaa aggctcggga agatcaccgg ctgccctgcc aggcggcgat actgcttggg
      121 caagtcgccg ccggcacgca atccgcgtcc cgctgcaaca acgacggccg ccaccctgtc
      181 cgacatgctc gaaagtccaa cctgcaaacc cacatcgaat tagcgcgttg cggcggcagg
      241 gcaaactgtt tcggtcggca gcgccgcttg cgccggcctg gccaaaactt ttgcactgca
      301 acacttgcga aaaatttcat cttggctaaa ctctatgcag tcatggcgag cgcctaattc
      361 gtgtgcagga aagttaagtg cctcactgtc aagagaaatt caggtcggac gggcttgaga
      421 tcggcggcat tcgcctgccg aaccgggtgg cgctggcgcc gatgtcgggc gtcaccgacg
      481 cgcccttccg ccgcatgacg gctcggctgg gtgcgggcct ggtcgtttcc gaaatgaccg
      541 ccagccagga cctcgtggaa ggctgcccgc gcacgcgcct gcgcgcggaa cgcgccgggg
      601 tgggcatcca tgtcgtccag ctcgcgggct gcgagcccca ttggatggcc gagggcgccc
      661 gcatggccga gagcgagggc gccgacatcg tcgatatcaa catgggttgc ccggcgcgcc
      721 acgtcaccgg cggacaggcc gggtcggcgc tgatgcgcga tctcgatcac gcgctgacgc
      781 tcattgaggc gacaatcgcc gccgtttcgg tgccagtgac gctcaagatg cggcttggct
      841 gggacgagcg cagcatcaac gcccccgagc ttgcgcgccg agcggaagcg gcaggcgtgc
      901 gccttatcac cgtgcacggc cgcacccgct gccaattcta caagggccgc gccgactggg
      961 gcgtcatccg cgccgtggtc gacagcgtcg ccattcctgt actggtcaac ggcgacatcg
     1021 aaaccttcga tgacgtcgat gcggcgctgg caatgtcagg cgccgatgcg gtgatgctcg
     1081 gccgcgccgt gcagggccgc ccctggctgc ccgggcaggt ggcccgctat ctcgcgagcg
     1141 gacaacggca gggcccgccg ccgctggcga tccaattcga catcgtcaat gagctctatg
     1201 aggccatgct cacgcatcac gggctggcgg ttggtcgccg tcatgcccgc aagcaccttg
     1261 gctgggcgct cgatgcggcc gcgcacagca ccggtgcacc agccgatcgc ctcaagtctt
     1321 ttcgccagcg cgtcctcacc gaggacgatc cctgcgcggt gcgccggcat ctggccgatg
     1381 ccttcgacgc gatggactcg gcgagggccg cggcatgaat cgactcgagc cgatgccgtt
     1441 tccgtccgga tcggccgacg ccgtgctcga tgcgctgcct catcccgtga tcatggtggc
     1501 tgccgatggc aagctcgcca atgccaacgc agcggctgaa tcctttttcg aggcctcgct
     1561 tccggtg
//
LOCUS       DASTMT010000296         8387 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_2453674, whole genome shotgun sequence.
ACCESSION   DASTMT010000296 DASTMT010000000
VERSION     DASTMT010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 8387)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8387)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8387
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2453674"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..435)
                     /gene="folK"
                     /locus_tag="VFR00_06805"
     CDS             complement(<1..435)
                     /gene="folK"
                     /locus_tag="VFR00_06805"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEU0159002.1"
                     /translation="MIKNTPNVYLYRLKFSQSGPSCGSNCLFLGLGANIAGRWGSPRQ
                     TLARALAALGRAGLRPLACSRFLRTKPVGGGYQPDFLNAVVQTQARLAPIALLRVLKG
                     LERQAGRRPGRRAGPRPLDIDILDYGGRRCGGSGAQRGRARLI"
     gene            565..1212
                     /locus_tag="VFR00_06810"
     CDS             565..1212
                     /locus_tag="VFR00_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012111787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEU0159003.1"
                     /translation="MHFYETERIALFIDGANLYATAKALGFDIDYKRLLAMFRTKGQL
                     VRALYYTALAEDQEYSSIRPLVDWLDYNGYTMVTKPTKEFTDALGRRKIKGNMDIELT
                     VDAMRLVDRLDHIVLFTGDGDFRALVAALQQQGRRVSVVSTLQTQPPMVADELRRQAD
                     QFIDLADLEDQVCRDPASRPMREPREGGSRPSPARRQAPAPATLNYPDEEDDERP"
     gene            1239..1880
                     /locus_tag="VFR00_06815"
     CDS             1239..1880
                     /locus_tag="VFR00_06815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012111786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEU0159004.1"
                     /translation="MLDRPAEAQADCPLCPRLVAFRGRNQSAEPGWFNGAVPSFGPAD
                     ARLLIVGLAPGLRGANRTGRPFTGDYAGDLLYASLLAFGWARGSYAAKPDDGLVLVDC
                     MITNAVRCVPPENKPTPAEITTCRRFLAGRIANLPRLSAILALGRIAHDSTLDALGAR
                     KSAFPFRHGAQHQAGDGVRLFDSYHCSRYNTNTGRLTNEMFTAVLAAIKTHLG"
     gene            complement(1924..2472)
                     /locus_tag="VFR00_06820"
     CDS             complement(1924..2472)
                     /locus_tag="VFR00_06820"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEU0159005.1"
                     /translation="MREAPPAADDGAARHLRRGRRMPELSLPATSGGAVNLAGLSGRV
                     ILYCYPWTGRPGLPNPEGWDEIPGAHGSTPETEGFRDLYAGFRQVGAEVFGLSTQESA
                     YQREMAERLRLPFALLSDANFALQGALALPTFSTGGVTYLKRLTLALKDGVIERVYYP
                     VHPPAAHAREVCAWLGMIDRTH"
     gene            complement(3076..4179)
                     /locus_tag="VFR00_06825"
     CDS             complement(3076..4179)
                     /locus_tag="VFR00_06825"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HEU0159006.1"
                     /translation="MDPEIEITGMRVRPVRVPLRRTLNTRVGRFTHGPFLLIDLELKG
                     GGVGRVAGFTFIPLGQRIVPILLEELVASVKGRRIGLAEVPQVHDACQRRLSHLGHEG
                     LAQMAISMFDMALHDALAREASLPLYRLLGGSKQPLPTYHSCGLGIAEPLEVAREARE
                     MRGEHGGFTHLKLRLGRERADDDVAAVKAVLSALGPEVTISVDFNQGLPVARALETCR
                     AIDNFGLAWIEEPVAYDDYDTQARLARKLATPLQVGENWWSWRVGKAAIEQGATDYVM
                     PDLLRIGGVTGWLRLARVAEAHSVPFSSHLSPDFSAHVLAATPTKHWLEFMDWGQDLM
                     LDPLLPVAGFTTPPDRPGTGVEWNEPAIARCLI"
     gene            complement(4281..5153)
                     /locus_tag="VFR00_06830"
     CDS             complement(4281..5153)
                     /locus_tag="VFR00_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU0159007.1"
                     /translation="MATLAGKTLFITGASRGIGLAIALRAARDGANIAIASKTTEAHP
                     KLEGTIYSAADAIEKAGGKALPLKCDIRFEAEVAAAVAQTAKTFGGIDICVNNASAIS
                     LTGAQATDMKRYDLMHGINARGTFVVSKACIGHLRQAANPHILMLSPPLDMNVKWFAP
                     HVAYSMAKFNMSIIALGLAGELKGDGIAVNTLWPRTTIATAAVKNLLGGDALIRRSRT
                     PDIMADAAHLVLTQDAKSFTGQFLIDDTLLYAHGVTDFERYRVDKDAELAPDFFVPST
                     IPPPPGVTLAERLR"
     gene            complement(5312..6931)
                     /locus_tag="VFR00_06835"
     CDS             complement(5312..6931)
                     /locus_tag="VFR00_06835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0159008.1"
                     /translation="MGLLGALLLVPSSVKADWLGTLVGTATKWVAAAPQGAGSSLRAT
                     RALDAAFAYIKRLPPKPEATVLAAESTQEGHWRFVNRAGETITAGTPEEMKRVVSLLV
                     PDARAEARLTLYLTEDTVFTRRATLKDLPRGSELNLCAGDESYRLVRRSEASSDRLFA
                     EIRPNLVVELTAAKDFAEAAWQLARPLDKASIRVLALEAGSATRLASSPRIDPVSKKA
                     LIDSIDPPSLAAALGAVRGQTMLVAGRVEGELLYFKGASGPERSLLVRDLYGAAEQAD
                     VNLIVLHASSTPRQPGGRNWLWQKVEVKGLEEALQRARVADFYNALATPTSRYLVTAK
                     ALSAQRTLLQVTPIADLPRVVASRPLSDILGDAAASLTGRVIMAGVEANIRSAAQQKD
                     VDWRLLPGVPATLQLGYLGLLLLGLLGAPVARTWWGEVWPREDPAEYADRRGYWAARL
                     VRDCLFVLLFLPLTAAFSGPLNLGRQIRDALLAPIRAWRWLRGGWRRSPHDAAGGSGD
                     AEIGAAATLGPPLRAERAAGSGSLGHYRPSR"
     gene            complement(7094..>8387)
                     /locus_tag="VFR00_06840"
     CDS             complement(7094..>8387)
                     /locus_tag="VFR00_06840"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018270432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEU0159009.1"
                     /translation="FKSRVVSAHFQDASATWEVNLADGARHRARFLITAIGLLSAPTL
                     PRLAGLDSFRGQSFHTAAWPHTPVSFAGKRVAVLGTGATGVQTIQEVAKTAGHLSVLQ
                     RTPNWCAPLHNGSIDAASQRQIKASYPEIFARCRETFACFLHTPDPRATFEVSAEERE
                     AFFEKLYAERGFGIWQGNFRDILTDRAANAAISAFVARKIRARVHDPEVADKLIPKDH
                     GFGTRRVPLETSYYEVYNQPNVRLVDLRQTPIERITPDGLETSAATHAFDIIIYATGF
                     DAITGSFDRIDIRGRDGHPLKAKWANGPKTFLGVHVEGFPNMFMLMGPHTALGNIPRS
                     IEFNVEWVAGLIGHMRQRRLTRADAAPQAVADWTDFVLQMGRGLLSNEVDSWMTGVNS
                     NVEGKQTRIVARYSGSAPAYRQRCEEVAAQGYRELALS"
BASE COUNT         1355 a         2780 c         2917 g         1335 t
ORIGIN      
        1 gatcaggcgg gcgcggccgc gctgggcgcc ggacccgccg cagcgccggc cgccatagtc
       61 caagatgtcg atgtcgaggg ggcgcggccc ggcgcgccgg cccgggcggc ggccggcttg
      121 ccgttccagg cctttcagga cgcgcaaaag ggctatgggt gccagcctgg cctgggtttg
      181 cacgacggca ttgagaaagt cgggctggta gcccccgcca acgggcttgg tgcgcagaaa
      241 gcgcgagcac gcaagcgggc gcaggcccgc ccgccccagg gccgccagcg cacgcgccag
      301 cgtttgccgg ggactgcccc aacgaccggc gatgttggcg ccgagcccta aaaataggca
      361 atttgatcca cagctcggcc ctgattgcga aaatttaagt ctatacaagt agacatttgg
      421 ggtattcttg atcatggcta tttgtcgcct tggcattgct gctgaatgcg cttacgtcac
      481 acctcttcat caggctgctc gcgccgacgt tagcgcggct agcgacccgc gggatttttc
      541 agaggactaa cccaaatgca ttcaatgcat ttttacgaga ctgagcgtat tgccctgttc
      601 attgacggcg ccaacctcta tgccacggcc aaggcgcttg gtttcgacat cgattacaag
      661 cgcttgttgg cgatgtttcg caccaaaggt caactcgtgc gggcgctcta ctacaccgcg
      721 cttgccgaag accaggagta ttcctcgatc cggccgctgg tcgattggct cgactacaac
      781 ggctacacca tggtgaccaa gccaaccaag gagttcaccg acgccttggg ccgccgcaag
      841 atcaagggca acatggatat cgagttgacc gtcgatgcca tgcgcctggt cgaccgcttg
      901 gatcacatcg tcctcttcac cggggatggc gattttcgcg ccctggtggc ggccttgcag
      961 caacaggggc gccgcgtcag cgtcgtctcg accctgcaga cgcagccccc gatggtggcc
     1021 gacgaattgc gccggcaggc ggatcagttc atcgacctcg ccgatctaga agatcaggtc
     1081 tgccgcgatc cggcgtcgcg gcccatgcgc gagccgcgcg agggtggttc ccgcccgagc
     1141 ccggcgcggc gtcaggcacc ggcgccggca acgctcaact atcccgacga ggaggatgac
     1201 gagcggcctt gagggagagc cgaggcggtg gggcggcgat gctggaccga ccggccgagg
     1261 cccaagctga ctgcccgctc tgtccgcgcc tcgtcgcctt tcgcggccgc aatcaaagcg
     1321 cggagcctgg ctggttcaac ggcgcggtgc cgtcgttcgg ccctgccgac gcgcgtctct
     1381 tgatcgtcgg cttggccccc ggtttgcgcg gggccaaccg caccggtcgt ccgttcacgg
     1441 gcgattatgc cggcgatctg ctctatgcga gcttgctggc gttcggctgg gcgcgcggca
     1501 gctatgctgc caagcctgac gatgggctcg tcctcgtcga ctgcatgatc accaatgcgg
     1561 tgcgctgcgt gcccccggag aacaagccga ccccggcgga gatcacgacc tgtcgtcgct
     1621 tcctggccgg ccgcatcgcc aacctgcctc ggctgtcagc catcctggcg ctgggccgca
     1681 tcgcccacga cagcacgctg gacgcgctcg gcgcgcgcaa atcagccttt ccctttcggc
     1741 acggcgccca gcatcaggcc ggcgacggcg tgcggctgtt cgatagctat cactgctcgc
     1801 gctacaacac caacacgggc cggctgacga acgagatgtt taccgccgtg ctggcggcca
     1861 tcaagaccca tctgggctga aaagttcgcg cgccggtgcg ccaaggcgcg aggcccgtca
     1921 ccctcagtgg gtgcggtcga tcatgccgag ccaggcgcag acttcacggg cgtgcgcggc
     1981 cggcggatgc acgggatagt agacgcgctc gatgacgcca tccttgaggg cgagcgtgag
     2041 ccgcttcaga taggtcacgc cgccggtgct gaaggtcggc agcgccagcg cgccctgcag
     2101 ggcgaagttg gcatcgctca gcagggcgaa cggcagacgc aagcgttcgg ccatttcgcg
     2161 ctggtaagcg ctctcctggg tcgagagccc gaaaacctcg gcgccgactt ggcggaagcc
     2221 ggcatagagg tcgcgaaacc cttccgtctc cggcgtcgat ccatgcgcgc ccgggatctc
     2281 gtcccagccc tcggggttgg gcaggcccgg ccggccggtc cagggatagc agtagagaat
     2341 gacgcggcct gaaagccccg cgaggttcac ggcgccaccg ctggtcgccg gcaggctgag
     2401 ctcgggcatg cgccggccac gccgcagatg tcgggcggcg ccgtcgtcgg cggctggggg
     2461 tgcctcgcgc atggcaatcg gggctcctca ctgcgggggc caggggaggc gggcgtcccg
     2521 gaccgaggcc tgcccccgaa ccctgaccca gctcctggcg ggattaccgc gcttgctaga
     2581 taaaggccgg gcggcttggg cgctcgataa acgccggggc gcagggcggt caagttcgag
     2641 cggagcgcgc cgacgccgag cccagcaaaa aaggggcgcg caagaggcca agagcagggc
     2701 tatgccggga agcgcggggc gactaacgcg aggtgcgcag cgtccagctc aaaatgatag
     2761 caaccgtagc ggcgaggatc atcacctcgt acggcgcggt ccaactgtcg atccacgtga
     2821 atacggagcc gactaccgtc atgacgcact actcccccga cgacccgcgg cgccgagctt
     2881 ggcacaggcc ctgggccttg tcgagatcgt cagaggacag aggtgtccca ggggcagagc
     2941 cccccaggcc tctaggagcg cctccgacgc aacgcgaagc cgggtggacc gctattgctc
     3001 tttgcgaaga taatggtctc cccgtcccgg gcgcccgagg gacggggcgg caccggcccg
     3061 gcgccgccgc cgcccctaaa tcaggcagcg cgcgatcgcc ggctcgttcc attccacgcc
     3121 ggtgccgggg cgatcgggcg gggtcgtgaa gcccgcaacc ggcagcagcg gatcgagcat
     3181 caggtcttgc ccccagtcca tgaactccag ccagtgtttg gtcggggtgg cggccaggac
     3241 gtgggcggaa aaatcaggcg agaggtgcga agagaacggc acactgtgcg cctccgcgac
     3301 gcgggcgagc cgcagccacc ccgtgacgcc gccgatgcgc aggagatcgg gcatgacgta
     3361 gtcggtggcg ccctgttcga tggcggcctt gccgacgcgc caactccacc agttttcgcc
     3421 gacctgcagc ggggtcgcga gcttccgcgc caggcgcgcc tgggtgtcgt aatcgtcgta
     3481 ggcgacaggc tcctcgatcc aggcaaggcc aaaattgtcg atcgcccggc aggtctcgag
     3541 cgcgcgcgcc accggcaagc cctgattgaa gtcgaccgaa atcgtcacct cgggcccgag
     3601 cgcgctcaag accgccttca ccgccgcgac gtcatcgtcg gcgcgctcgc gccccagccg
     3661 cagtttcaag tgcgtgaagc cgccgtgctc cccgcgcatt tcgcgggcct cgcgggccac
     3721 ctcgagcggc tcggcgatgc ccaggccgca gctgtggtag gtcggcagcg gctgtttgct
     3781 gccgccaagc aggcgataga gcggcagcga ggcctcgcgg gccaaggcgt cgtggagcgc
     3841 catgtcgaac atggagatcg ccatctgcgc taggccctcg tgtcccagat gcgagagccg
     3901 gcgttggcag gcatcgtgca cctgcggcac ctccgcgagg ccaattctgc gtcccttcac
     3961 tgaggcaacg agctcctcga gcaggatcgg cactattcgc tggcccaggg gaataaaggt
     4021 aaagccggcg acgcggccga cgccgccacc cttcaactcc agatcgatca agagaaaagg
     4081 gccgtgcgtg aaacgtccaa cgcgggtgtt gagcgttcgc cgcagcggca cgcgcacggg
     4141 gcgcacgcgc atgccggtaa tctcgatctc gggatccatg tcgggcctcg tcgcatagcg
     4201 cctttcgatc ttcaagtcgc aaagagctcg gcggccgcca aggcggcgcc gcagccccgc
     4261 gagctcggca gcgctgcccg ctagcgcagg cgctcggcca gcgtgacacc gggcggcggt
     4321 gggatggtgc tgggcacgaa aaagtccggc gccaactcgg cgtccttgtc gacgcgatag
     4381 cgctcgaaat ccgtgacccc gtgcgcgtag agcagggtat cgtcgatcaa gaactgcccc
     4441 gtgaaggact tggcatcttg cgtcagcacg aggtgggccg catccgccat gatgtccggc
     4501 gtgcggctgc ggcgtatcaa ggcatcgccg cccaagaggt tcttgactgc cgcggtggca
     4561 atggtcgtgc gcggccacaa ggtgttgacg gcgatgccat cgcccttgag ctccccggcg
     4621 agacccaggg cgatgatgct catgttgaac ttggccatcg agtaggcaac gtgtggtgcg
     4681 aaccatttga cgttcatgtc gagcggcggc gacaacatga ggatgtgggg attggccgcc
     4741 tgccgcaaat ggccgatgca ggccttggaa acgacgaagg tgccgcgcgc gttgatgccg
     4801 tgcatcaggt cgtagcgctt catgtccgtg gcctgcgctc ccgtgagcga gatggcgctg
     4861 gcgttgttga cgcaaatgtc gatgccgccg aaggtcttgg ccgtctgcgc gaccgcggct
     4921 gccacctcag cctcgaagcg aatgtcgcat ttgagcggca gggctttgcc gcccgccttc
     4981 tcgatggcgt cggccgccga gtaaatcgtg ccctcgagtt ttgggtgggc ttcggtcgtc
     5041 ttcgaggcaa tggcgatgtt ggcgccatcg cgcgcggcgc gcagcgcaat cgccaggcca
     5101 atgccccgac tggcgccggt gatgaacagc gtcttgccgg caagggtcgc catctcgtcc
     5161 tccgtcggtt gtgctgtcat agctgggccg gcgccccccg gctaggggtg ggaggggccg
     5221 cggcagagcc gcgcccagcg ccgcgatccg gccgtgttgg gcacgcggcg acggcaagcg
     5281 tgcgcagcac caagcgcgcg gcgcgcgagc cttaccggct cggccggtag tggccgaggc
     5341 tccccgaacc ggccgccctc tccgcccgca aaggaggccc gagggtcgcc gctgcgccga
     5401 tctccgcgtc ccccgatccg ccggccgcgt cgtgcgggct gcggcgccag cctccccgca
     5461 gccagcgcca ggcgcggatc ggggccagca gcgcatcgcg gatctgacgg ccgagattga
     5521 gggggccgct gaaggccgcc gtcagcggca aaaaaagcag gacgaatagg cagtcgcgca
     5581 ccagccgtgc cgcccaatag ccgcggcggt cggcatactc ggccgggtcc tcgcgcggcc
     5641 acacctcacc ccaccaggtg cgcgcgacgg gcgcgcccag cagcccgagc agcagcagcc
     5701 cgagatagcc caattgcagc gtcgccggca cgccggggag caggcgccaa tcgacgtcct
     5761 tctgctgcgc ggcgctgcga atattggcct ccacgccggc catgatgacc cgtcccgtca
     5821 gcgaagcggc cgcatcgccc aagatgtcgc tgagcgggcg cgaggcgacg acgcgcggca
     5881 ggtcggcaat gggcgtgacc tgcagcagcg tgcgctgcgc gctcaacgct tttgccgtca
     5941 cgagatagcg gctggtgggc gtggcgagcg cgttatagaa gtcggccacg cgggcgcgct
     6001 gcagcgcctc ctccaggcct ttgacttcga ccttttgcca aagccagttg cgccccccgg
     6061 gctgacgcgg cgtcgaactg gcgtgcagca cgatgaggtt gacgtcggcc tgctcggccg
     6121 cgccgtagag atccctgacg agcaggctgc gctccggtcc gctcgcaccc ttgaagtata
     6181 agagttcgcc ctcgacacgg cccgccacaa gcatggtttg cccgcgcacg gcgcccagcg
     6241 ccgcggccaa acttggcgga tcgatgctgt cgatcaaggc tttcttgctg accgggtcga
     6301 tgcgcgggga ggacgcaagc cgcgttgcgc tgcccgcttc gagcgccagc acgcggatgc
     6361 ttgccttgtc caaggggcgg gcgagttgcc aggcggcctc ggcgaagtct ttggcagcgg
     6421 tcaactcgac caccaggttg ggacgaattt ccgcgaacag acggtcgctc gaggcctcgc
     6481 tgcggcgcac gaggcggtag ctttcgtcgc cggcgcagag attgagctcg ctgcccctcg
     6541 gcaggtcctt gagggtggcg cgccgcgtga agacggtgtc ctcggtcagg tagagcgtca
     6601 gccgcgcctc ggccctggca tcggggacga gcagggagac gacccgcttc atttcctccg
     6661 gcgtgccggc ggtgatggtt tcgccggccc tgttgacgaa gcgccagtga ccctcctggg
     6721 tcgattccgc ggccagcacc gtcgcctccg gcttgggcgg caggcgcttg atgtaggcga
     6781 aggcagcgtc gagcgcgcgc gtcgcgcgca agctgctgcc cgcgccttgc ggcgcggcgg
     6841 cgacccactt cgtggccgtc cccaccagcg tgcccagcca gtcggccttg acgctcgacg
     6901 gcacgagcag cagcgcgccc aggagcccca acgacgccag cagccccgct cgcgcacgcc
     6961 gccattgccg ccgtcgcggc gggggggcgt gggagtgcgg ttggcagata accatggcgc
     7021 gagcatatcg cagcattttg gacgaagcat ggcgctcacg ggcagcccgt ggtcggtgcc
     7081 cctgcggctc cggctagctc agcgccagtt cccggtagcc ctgcgcggcc acctcttcgc
     7141 agcgctggcg ataggccggt gcgctgccgc tatagcgggc gacaatgcgg gtctgcttgc
     7201 cctcgacgtt ggaattgacg ccggtcatcc aggaatcgac ctcgttggac aggaggccgc
     7261 gccccatctg caagacgaag tcggtccagt ccgccaccgc ctgcggcgcg gcatccgccc
     7321 gggtcaagcg acgctgccgc atatggccga tcaggcctgc cacccattcg acgttgaatt
     7381 cgatgctgcg gggaatgttg ccgagcgcgg tgtggggtcc catcagcatg aacatgttgg
     7441 ggaagccctc tacgtgcacg cccaggaagg tcttgggtcc gttcgcccat ttggccttga
     7501 ggggatggcc gtcgcggccg cgaatatcga tgcgatcgaa gctgcccgtg atggcatcga
     7561 agcccgtggc ataaatgatg atgtcgaagg cgtgcgtggc cgcgctggtc tcgagcccgt
     7621 cgggggtgat gcgctcgatc ggcgtttgcc gcaagtccac gaggcgcacg ttgggctggt
     7681 tatagacctc gtagtaggag gtctcgagcg gcacgcgccg ggtgccgaag ccatggtctt
     7741 tggggatgag cttgtcggcc acctccgggt cgtgcacgcg cgcgcggatc ttgcgggcca
     7801 caaaggccga gatggcggca ttggccgccc ggtcggtcag gatatcgcga aagttgccct
     7861 gccagatgcc gaagccgcgt tcggcataga gcttttcgaa aaaggcctcg cgctcctcgg
     7921 cactcacctc gaaggtcgcg cgtgggtcgg gcgtgtgcag gaagcaggcg aaggtctcgc
     7981 ggcagcgcgc aaagatctcg ggatagctgg ccttgatctg gcgttggctg gcggcgtcta
     8041 tgctgccgtt gtggagggga gcgcaccaat tgggcgtgcg ttgcagcacg ctcaagtggc
     8101 cggcggtctt ggccacctcc tggatggtct gcacccccgt ggcgccggtg ccgaggacgg
     8161 cgacgcgctt gcccgcaaag ctcaccggcg tgtgcggcca ggcggcggtg tggaaggatt
     8221 ggccgcggaa gctatcgagg ccggcgaggc gcggcagggt tggcgccgac aacaggccga
     8281 tggcggtaat cagaaagcgg gcccggtggc gtgcgccgtc cgccaaattc acctcccagg
     8341 tggcgctggc gtcctggaag tgggccgaga cgacgcggct cttgaag
//
LOCUS       DASTMT010000297         3239 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_818190, whole genome shotgun sequence.
ACCESSION   DASTMT010000297 DASTMT010000000
VERSION     DASTMT010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3239)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3239)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3239
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_818190"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(63..1835)
                     /locus_tag="VFR00_06845"
     CDS             complement(63..1835)
                     /locus_tag="VFR00_06845"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HEU0159010.1"
                     /translation="MTLKMSGAEMVIRALADQGVEHIFGYPGGAVLPIYDELFQQEKV
                     KHVLVRQEGGAVHAAEGYARSTGGVGVVLVTSGPGATNAVTGLTDALMDSIPVVCITG
                     QVPTHLIGNDAFQECDTVGITRPCTKHNWLVKNVEDLARILHEAFYVARTGRPGPVVV
                     DIPKDVQFAIGTYTGPKDVKHKTYQPRLRGDARAIAQAVELMAQAKRPVLYTGGGVIN
                     SGPKASQLLRELVRLTGFPITSTLLGLGAFPASGKEWLGMLGMHGTYEANLAMHDCDV
                     MVCIGARFDDRITGRIDQFSPRSKKIHIDIDPSSINKNVKVNVPVIGDVAYVLEELVR
                     AWKAKAVSVDKTALKTWWAQIDRWRAKKCLSYKNSDAFIMPQYAIQRLYELSQDRDTY
                     ITTEVGQHQMWAAQFFRFEQPNRWMTSGGLGTMGYGLPAAIGVQMAHPKSLVIDIAGD
                     ASILMNIQEMSTAVQYRLPVKVFILNNQYMGMVRQWQQLLHGNRISESYTAAMPDFVK
                     LAEAYGGIGIRCDKPGELDDAIKAMLKAPNDKPVLFDCRVAALANCFPMIPSGKAHHE
                     MLLGEEVTDEEIDKAVGKEGKVLV"
     gene            complement(1917..2852)
                     /gene="miaA"
                     /locus_tag="VFR00_06850"
     CDS             complement(1917..2852)
                     /gene="miaA"
                     /locus_tag="VFR00_06850"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEU0159011.1"
                     /translation="MPQQQRERAIVIAGPTASGKSQLALALAERLGGSIINADSMQVY
                     RELALLTARPGASELARVPHALYGCVSGREAYSAGRYAADAASAVAVARAAGRRPIIV
                     GGTGLYLRALLQGLSPVPAADPAVRAHWRQQVAARGSEELHALLASRDPAMAARLKPS
                     DPQRIVRALEVLDSTGRSLADWQRQAGRPVLAAGETTPLLLLPPPASHAAAIAERFQR
                     MLGAGALEEVRQLVALGLSPELPIMRALGVAALSSHLAGQQSLTAAAAAATTATRQYA
                     KRQMTWFRRNMISWKTFESQETESFIKEILAFIDA"
     gene            2935..>3239
                     /locus_tag="VFR00_06855"
     CDS             2935..>3239
                     /locus_tag="VFR00_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020091286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HEU0159012.1"
                     /translation="MKPVALVLTASPAAPILEAGLIAAMRPLLHPAELLRERWLAPGT
                     AWEGLLRSPAALPTRELTHRLGAIVAGRPVDLNVVPADPAGRRKKLLVADMESTIIE"
BASE COUNT          528 a         1083 c         1072 g          556 t
ORIGIN      
        1 ctcggcgccg gccgcctcgg cttgcccgtg ccgaaacggc ccgtgccgta gaacgtgccg
       61 cctcagacca acaccttgcc ttccttgccg acggccttgt cgatctcctc gtccgttacc
      121 tcctcgccca gcagcatctc gtggtgcgcc ttgccggagg ggatcatcgg gaagcagttg
      181 gcgagggcgg cgacgcgaca atcgaacagc accggcttgt catttggcgc cttcagcatg
      241 gccttgatgg catcgtcgag ctcgccgggc ttgtcgcacc ggatgccaat gccgccgtaa
      301 gcctcggcga gcttcacgaa gtcgggcatg gcagccgtgt agctctcgga gatgcggttg
      361 ccgtgcaaca gctgctgcca ctgccgcacc atgcccatgt actggttatt gaggatgaag
      421 accttgacgg gcaaccggta ctgcacggcc gtcgacatct cctggatgtt catcaggatc
      481 gaggcatcgc cggcaatgtc gatgacgagc gacttgggat gagccatctg cacgccgatg
      541 gcggccggca agccgtagcc catggtcccg agcccgcccg aggtcatcca acggttcggc
      601 tgctcgaagc ggaagaactg tgccgcccac atctgatgct ggccaacttc ggtggtgatg
      661 taggtgtcgc gatcctggct gagctcatac agccgctgga tggcatactg gggcatgatg
      721 aaggcatcgg aattcttgta gctcaggcac ttcttggcgc gccagcgatc gatctgggcc
      781 caccaggtct taagcgcggt cttgtcgacg ctgaccgcct ttgccttcca agcgcgcacc
      841 agctcctcga gtacgtaggc gacgtcaccg atgaccggca cgttgacctt gacgttcttg
      901 ttgatcgagg agggatcgat atcgatgtgg atcttcttcg agcgcggcga gaattggtcg
      961 atgcggcccg tgatccgatc gtcgaagcgc gcaccaatgc acaccatgac atcgcaatcg
     1021 tgcatggcaa gattggcctc gtaggtgccg tgcatgccga gcatcccgag ccactccttg
     1081 cccgaggccg gaaaggcccc gaggcccaat agcgtagagg tgatcggaaa acccgttagc
     1141 cggaccagct cgcgcagcag ctgactggcc ttcggccccg aattgataac gccgccaccg
     1201 gtgtagagca cgggccgctt tgcctgcgcc atcagctcga cggcttgggc gatggcgcgc
     1261 gcatcgccgc gcaggcgcgg ctgataggtc ttgtgcttga cgtccttcgg ccccgtgtag
     1321 gtgccgatgg cgaattgcac atccttggga atgtcgacca ccaccggccc gggtcggccg
     1381 gtgcgcgcga cgtagaaggc ctcatgcaag atgcgcgcga ggtcctcgac gtttttgacg
     1441 agccaattgt gtttggtgca gggtcgggta atgccgacgg tgtcgcactc ttggaaggca
     1501 tcgttgccga tgagatgcgt cggcacctgg ccggtgatgc acaccaccgg gatcgaatcc
     1561 atgagcgcgt cggtcaggcc cgtaacggca ttggtcgcgc cggggcccga cgtcaccaag
     1621 accacgccga ccccgcccgt cgagcgcgcg tagccttccg ccgcatgcac cgcgcccccc
     1681 tcctggcgca ccaagacgtg cttgaccttc tcctgttgga agagctcgtc atagatcggc
     1741 agcaccgcgc cgccggggta gccgaagatg tgctcgacgc cctgatcggc cagcgcgcga
     1801 atgaccattt cggcgccgct cattttgagt gtcatggctc gagctccgtt acgcaaaccc
     1861 gcccgatcgg agagggccgc gcattgatag gggcgggcat agtggcgggt caaccgctaa
     1921 gcatcaatga atgccaaaat ttccttgatg aaactttccg tttcttgcga ttcgaatgtc
     1981 ttccacgaaa tcatatttcg tctaaaccag gtcatctgcc gtttggcgta ctggcgcgtg
     2041 gccgtggtgg ccgcggccgc cgcggcggtc aggctctgct ggccggccag atgggagctc
     2101 agggctgcga ccccaagcgc gcgcatgatc ggcagctcgg gggagagccc gagcgctacc
     2161 agctggcgca cttcctccag cgcccccgca cccagcatcc gctgaaagcg ttccgcaata
     2221 gcggcggcat ggctggcggg cggcggcagc agcagcagcg gggtggtctc gcccgccgcg
     2281 agcaccggcc ggccggcttg gcgctgccag tcggccaacg accggcccgt gctgtcgagg
     2341 acctcgagcg cgcgcacgat gcgttgcgga tcgctgggct tgagcctcgc cgccatggcg
     2401 gggtcgcgcg aggccaacag cgcgtgcagc tcctcagagc cgcgcgcggc cacctgttgg
     2461 cgccagtggg cgcgcacggc cgggtcggcg gcgggcacgg gagacagccc ctgcaaaagg
     2521 gcgcgcaagt aaagccccgt gccgcccacg atgatgggtc gacgcccggc cgctcgcgcc
     2581 acggccacgg cgctggcggc atcggccgca tagcgcccag ccgagtaggc ctcccggccg
     2641 ctgacgcagc cataaagcgc gtgcgggacg cgcgcgagtt cgctggcacc ggggcgggcg
     2701 gtcaagagcg cgagctcccg gtagacctgc atgctgtcgg cgttgatgat gctgccgccg
     2761 agccgctccg ccagggccag cgcgagctgg gacttgccgc tggccgtcgg cccggcaata
     2821 acgatggcgc gctctcgctg ctgctgcggc atgggggcca attaggctgc tcccccggtc
     2881 aaatcaactg cggcgcgggc gagcgcgccg gcgccgcgac ggacgatgct tgccatgaaa
     2941 cctgttgccc tcgtgctgac ggcctcgccc gcggcgccca tcctcgaggc agggctgatc
     3001 gccgccatgc gcccgctgct gcaccccgct gagctcctac gggaacgctg gcttgccccg
     3061 gggacggcct gggagggcct gctgcgcagc ccggctgccc tgccgacgcg cgagctgacc
     3121 catcgccttg gcgccatcgt ggcaggccga cccgtcgatc tcaatgtcgt tcccgccgac
     3181 ccggcgggcc ggcgcaagaa gctgctcgtc gccgacatgg aatcgacaat catcgagca
//
LOCUS       DASTMT010000298         6566 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_5333585, whole genome shotgun sequence.
ACCESSION   DASTMT010000298 DASTMT010000000
VERSION     DASTMT010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 6566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6566
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5333585"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            407..2908
                     /locus_tag="VFR00_06860"
     CDS             407..2908
                     /locus_tag="VFR00_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEU0159013.1"
                     /translation="MAELVFLVLCVAGAFALAMHRARLWAWAAALAGATCIWQTGLLH
                     GEFEEPAFGALGLLAWVPVVVLAGLSITAVRRAALIAPIFRSIKAVLPKVSATEQEAL
                     DAGTIGFDAELFSGTPDWQKLRDVPPIMLSEEEKAFLDGPTSALCRLVDDWAIRHNLK
                     EIPAEVWSFIKQHGFLGMLISKEHGGLGFSAQAQSLVLGRIASRSPDVCTLVMVPNSL
                     GPGELIEKYGTPAQKEHFLPRLANGSEVPCFALTGPTSGSDAATMRDVGYVAKGRHAG
                     QEVIGIRASWEKRYITLAPQATLIGLALRLIDRDNLLGRGEDVGITLVLVPADHAGVE
                     IGRRHLPSGAAFPNGPIWGHDVFIPIDWVIGGEKMVGQGWRMLMECLAAGRSISLPSS
                     AAAGAKSLLRNASAYARIRKQFDLPIGKMEGLQEPLARLVENAYVIEAARAVTAAMVS
                     RGEKPSVLSAIMKYQTTERMRRSVNDAMDLHGGRAICDGPTNYLQSAYQMMPVAITVE
                     GANILTRSLITFAQGALRSHPYLYKEIEACQDSNPERGLAQFDAAFCGHVAFSFGNTC
                     GALFHNLTFGLFGQVPDKAYGTAEWYRQLWRAARNFAFVADLTVAVLGGGLKVKQKLT
                     GRLADALSELYFLACVLKRYEDDAKPANDRHIVALAASNGLFRFQEALRGVIDNFPVA
                     PVRWLMRGVVFPFGSHYRPAPDWLGQRVTELVLVPGEVRERLTRFIFVSRNPNDPTGL
                     LEVTFAKAVEAEAAERKLDRAVRDGTVRRVHGNDWIGEAVAKQVLSDSEGQMLRELEK
                     LTQRAIAVDHFDAAEVRPHYMTPGHNARAMQSAAE"
     gene            2916..4925
                     /locus_tag="VFR00_06865"
     CDS             2916..4925
                     /locus_tag="VFR00_06865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013418529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HEU0159014.1"
                     /translation="MMAELLSLRDWRCSIDPQGIAWAVFDREGESQNSLGQRPLEELA
                     QIVDWAERGARERSIRGLVIGSGKEKGFIVGADVREFEAITSEQQVIDGVSLVNTLFD
                     RIERMPVPVVCTVHGFCLGGGLELALACHWRIATNDPATRLGFPEVRLGIFPGFNGTA
                     RSIRQAGALSAMQIMLTGSMLRAGAARGMGLIDELVASPLNLAWAARRAIQRNRKSKA
                     AGGWQALLRFAPARGLLAKRLRTETGKKVRQEHYPAPFRLIDLFEAHAGDLEAMKLAE
                     TRAFAPLMLSDTSRNLRRVFKLSELLKAQAPKDLKFKPLRVHVIGAGVMGADIAGWCV
                     ASGMEVSLQDLNEEQLKKGREAQKKLFGRKFRTRAQREAAMARLISDPKGFNIPRADV
                     VIEAIVEKLESKQALLKSIEAKLKPGAVLATNTSSIEIEKIAEALSEPGRLIGIHFFN
                     PVAQMPLVEVVRGAQSEPEQVAKGCIFVTSVDKFPLIVKSNPGFLVNRVLAPYMLSAL
                     QRLERGEAKEKIDEAARAFGMPMGPIELADTVGLDVCAHVGSILKLAPAGASKLDQMV
                     AAGKLGKKSGEGFYVWKNGKAEKSKPEQPYEAFELERLGRELVDPLIRECERCRDEKI
                     VDSADLVDAGVIFGTGFAPFRGGPLHFRANEQKSNVEPLRRTAAA"
     gene            4992..6296
                     /locus_tag="VFR00_06870"
     CDS             4992..6296
                     /locus_tag="VFR00_06870"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HEU0159015.1"
                     /translation="MEQTLRRVGVVGGVRIPFCRSNTLYRDQSNLAMLTATLNGLVDR
                     YNLQGVHIDEAIGGAVVTHSKDWNLAREAVLGSKLAPSTPGITLQQACGTSLQAAMIL
                     QAKIATGQIECGIAMGSDTTSDAPIVFKEKFAHRLVEAQNAKSFGQRMAAFKGFSPAE
                     LAPQPPSVSEPRTGLSMGQHTELMAQEWHISRSEQDRFALESHRKAAEAYRSGYLDDL
                     VVPFAGVFRDNNLREDASLDRLATLKPAFDKSERGTLTAANSTPLTDGAACVLLASET
                     WAASRALPIEAYLTHAQTAANNFVAGDGLLLAPTIAVSRLLERAQLTLQDFDYYEIHE
                     AFAAQVLVTLKAFEDAAYCKSVLGRDAPLGSIDRAKLNVKGSSLAFGHPFAATGARII
                     ANLGKLLAVNGGRGLISVCTAGGMGVAAIMESARAAALQAAA"
     gene            complement(6378..6527)
                     /locus_tag="VFR00_06875"
     CDS             complement(6378..6527)
                     /locus_tag="VFR00_06875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0159016.1"
                     /translation="MRTIVGALLGLSLLAGMAASASAADCKVVGWVDSGQGGRPIFSC
                     TDQGR"
BASE COUNT         1130 a         2089 c         2180 g         1167 t
ORIGIN      
        1 aatggctgcc cccaagcgcc gcccaggggc cccgatgtgt gctcgggtgc ccccgtcgcc
       61 agcgccaagc tccaggtttt ggcgcgcgtt ggcgaaggcg ttatactgca aggggtggat
      121 cgccggctgc agctgaaaag gcggggtgtg cgcttccgcg agcctggacg gcggcgccag
      181 ggttgcgcca acctgttggg aggttggggg ccgtcctcgt cgcctgcccc ggcgcagggt
      241 gccgttcttg ccgtggggtc tggcgcgcga caggtcgtcc tcgctgcagc agggctgagg
      301 ctataagcgg gtttgaggtc tgagccgggt cgcaggtcct gagcggggtc gaccgccttg
      361 aaattaaggg cttgggtata gtttaggtgg gaaactggag gccatcatgg cggagcttgt
      421 gttcctcgtt ctctgtgtgg cgggcgcctt cgcgctggcc atgcaccggg cgcggctgtg
      481 ggcctgggca gcggcgcttg ccggtgcaac ttgcatctgg cagacgggcc tgctgcacgg
      541 cgaattcgag gagcccgcct tcggcgcgct cggcttgttg gcctgggtgc cggtggtggt
      601 gctggccggt ctgtcgatca ctgccgtgcg ccgtgccgcg ctgatcgcgc ccatcttccg
      661 cagcatcaag gccgtactgc ccaaggtctc cgccacggaa caggaggccc tcgatgcggg
      721 caccattggg tttgatgcgg agctcttctc cggcacgccg gattggcaga agctgcgcga
      781 tgtgcccccg atcatgctca gcgaggagga gaaggcgttc ctcgatggcc cgaccagcgc
      841 gctctgccgg ctggtcgatg attgggccat tcgccacaac ctgaaggaaa ttccagccga
      901 ggtttggagc ttcatcaagc agcacggctt tctcggcatg ctgatttcca aggagcacgg
      961 cgggctcggc ttctccgcgc aggctcaatc cctggtgctc ggtcgcattg cctcgcgctc
     1021 gcccgacgtt tgcacccttg tcatggtgcc gaattccttg gggccgggtg aactgatcga
     1081 gaaatacggc acgccggcgc agaaggagca cttcctgccg cggctcgcca acggcagcga
     1141 ggttccctgc tttgcgctga ccggtcccac ctccggctcc gatgcggcca ccatgcgcga
     1201 cgtcggctac gtcgcgaagg gccggcacgc aggccaagag gtaatcggca tcagagcgtc
     1261 ctgggagaag cgctacatca cgctcgcccc gcaggcgacc ctgatcggcc tcgccctacg
     1321 gctcatcgac cgggacaacc tgttgggccg cggcgaagac gtgggcatca cgctcgtgct
     1381 cgtgcccgcc gatcatgccg gcgtcgaaat cggccgccgg catctgccga gcggcgccgc
     1441 tttcccgaac ggcccgatct gggggcacga cgtcttcatt cccatcgact gggtaattgg
     1501 cggcgagaaa atggtgggcc agggttggcg gatgctgatg gagtgtctgg ccgcgggccg
     1561 ctccatttcg ctaccgtcgt cggcggcggc aggcgcaaag tcgctgctgc gcaatgccag
     1621 cgcctatgcc cgcatccgca agcagttcga cctgccgatc ggcaagatgg agggtttgca
     1681 ggagccgctg gcacgcctgg tcgagaatgc ctacgtgatc gaggcggcgc gggcggtgac
     1741 cgccgccatg gtgagccggg gcgaaaaacc ctccgtgctc tccgccatca tgaagtatca
     1801 gaccaccgaa cgcatgcgcc ggtcggtcaa cgatgccatg gacctgcatg gcggccgcgc
     1861 catttgcgac gggcccacga attacctgca gtcggcctac cagatgatgc cggtggccat
     1921 cacggttgaa ggcgccaata ttctcacgcg ctccctcatc acctttgcgc aaggcgcctt
     1981 gcgctcgcat ccctatctct acaaggagat tgaggcctgc caggattcaa atcccgaacg
     2041 cggtctggcg cagttcgacg ccgcattttg cgggcacgtg gccttctcgt tcggcaatac
     2101 ctgcggcgcc ctgtttcata atcttacctt cggtttgttc ggccaggtgc ccgacaaagc
     2161 ctatggtacg gcggaatggt accggcagct gtggcgggcc gcccgcaact ttgcctttgt
     2221 cgccgacctg accgtggcgg tgctgggcgg aggcctcaaa gtcaaacaga agctgacagg
     2281 gcgtctggcc gacgcgctct ccgagctcta ttttctcgcc tgcgtgctca agcgctacga
     2341 ggacgatgcc aagccggcaa acgaccgaca catcgtggcg ctcgccgcca gcaacggcct
     2401 tttccgcttc caggaggcgc tgcgcggcgt gatcgacaac ttccccgtcg ctccggtgcg
     2461 ttggttgatg cgcggggtcg tgttcccctt cggtagccac taccgtcccg cgcccgattg
     2521 gctcgggcag cgcgtcaccg agcttgtgct ggtgcccggc gaggtgcgtg aacgcctgac
     2581 ccggttcatt tttgtctcac gcaatcccaa cgatcccacc ggcctcttgg aagtgacctt
     2641 tgccaaggcg gtcgaagcgg aagctgcgga acgcaagctc gaccgcgccg tgcgcgacgg
     2701 caccgtgcgg cgtgtgcatg gcaacgattg gatcggcgag gcagttgcca agcaggtctt
     2761 gagcgatagc gaagggcaaa tgctgcgcga gctggaaaag ctgacgcagc gcgcgattgc
     2821 cgtggaccat ttcgatgcgg ccgaggtgcg gccccattac atgacgcccg gtcacaacgc
     2881 gcgggcgatg caaagtgcgg cggagtgagg caaacatgat ggccgaattg ctgagcttgc
     2941 gggactggcg ctgttcgatc gatccgcagg gtatcgcttg ggcggtgttc gaccgcgaag
     3001 gcgaaagtca gaactcgctg ggccagcgcc cgctcgagga gctggcgcaa atcgttgatt
     3061 gggccgagcg cggcgcgcga gagcgcagca tccgcgggct ggtcatcggc tcgggcaagg
     3121 agaagggctt tatcgtcggc gccgacgtgc gcgagttcga agccattacc agcgagcaac
     3181 aggtgatcga cggggtgagc ctcgtcaaca ccctgttcga tcgtatcgag cgcatgccgg
     3241 tgccggtcgt atgcacggtg catggcttct gcttgggtgg aggcctcgag cttgcgcttg
     3301 cctgtcattg gcgcattgcc acgaacgatc cggcgacgcg gctgggcttt cccgaggtca
     3361 ggctcggcat cttcccgggc ttcaatggca ccgcgcgttc catccgtcag gccggcgcgc
     3421 tcagcgccat gcagatcatg ctgacgggtt cgatgctacg ggccggcgcc gcccgcggca
     3481 tgggtctcat cgacgagttg gtggcaagtc cgctcaatct ggcgtgggcg gcgcgccgcg
     3541 ccatccaaag aaaccgcaaa tcgaaggcgg ccgggggctg gcaggcgctg ttgcgcttcg
     3601 ctccggcgcg cggcttgttg gccaagaggc tgcgcacgga gaccggcaag aaggtgcgcc
     3661 aagagcacta tcccgcgccc tttcgtctga tcgacctgtt cgaagcgcac gcgggcgacc
     3721 tcgaggccat gaagctcgcc gagacgcgcg cctttgcccc gctgatgctg agcgatacct
     3781 cgcgcaacct gcgccgggtc ttcaagctct ccgagctcct gaaggcgcaa gcacccaagg
     3841 atctaaagtt caagccgctg cgcgtgcatg tgatcggcgc cggcgtgatg ggtgccgaca
     3901 ttgccggctg gtgtgtggca tccggcatgg aagtgtcgct gcaagacctg aacgaagagc
     3961 agctcaaaaa gggccgcgag gcccagaaga agctgttcgg gcgcaagttc agaaccaggg
     4021 cgcaacgcga ggccgccatg gcgcgcctga tctccgaccc caagggcttt aacattccgc
     4081 gcgccgacgt ggtgatcgag gccatcgtcg agaagctcga gagcaagcaa gctctgctca
     4141 agtccatcga ggcaaaactg aagccgggtg cggtgctggc gaccaacacc tcctcgatcg
     4201 agatcgaaaa gatcgccgag gcgctgagcg agccaggtcg cctcatcggc atacacttct
     4261 tcaatccggt ggcgcagatg cccctcgtgg aggttgtgcg cggcgcccaa agcgagcccg
     4321 agcaggtggc gaagggctgc atcttcgtca cctccgtcga caagtttccc ctgatcgtga
     4381 agagcaatcc gggcttcctc gtcaatcgcg tgctggcgcc ttacatgttg tcggccctgc
     4441 agcgcctgga gcgcggcgag gccaaggaga agattgacga ggcggcccgc gcgttcggca
     4501 tgccgatggg accgatcgag cttgccgaca ccgtcgggct cgatgtctgc gcccacgtcg
     4561 gcagcatcct caagctggcg ccggcgggcg ccagcaagct cgatcagatg gttgccgccg
     4621 gcaagctcgg caagaaatcg ggcgaaggtt tctacgtgtg gaagaatggc aaagccgaaa
     4681 agagcaaacc cgagcagccc tatgaggcct tcgagctcga gcgcttgggg cgcgagctgg
     4741 tcgacccgct gatccgcgaa tgcgaacgct gccgtgacga aaagatcgtc gatagcgccg
     4801 atctggtcga tgcgggggtg attttcggca ccggctttgc gccattccgg ggcggcccgc
     4861 tgcacttccg ggccaacgag cagaaatcca acgtcgagcc cttgcggcgc accgcggccg
     4921 cctgaatcgg ctgcggcgcg ggggacgaag aggtttgccg ccggccgggc ggcagcgaag
     4981 gagctggagc gatggaacag acactgcggc gggttggcgt ggtcgggggc gtacgcatcc
     5041 cgttctgccg gtccaacacg ctttatcgcg atcaatccaa cctggcgatg ctgacggcga
     5101 cgctgaacgg gctcgtcgat cgatataatc tgcagggcgt gcacatcgac gaggcgatcg
     5161 ggggtgccgt cgtcacgcat tccaaggact ggaatttggc gcgcgaggcc gtgctgggca
     5221 gcaagctggc accgtcgacg cccggcatca ccttgcagca agcctgcggc accagtctgc
     5281 aggcggccat gatcctgcaa gcgaagatcg caacgggaca aatcgaatgc ggcattgcca
     5341 tgggctccga caccacctcg gacgcgccga tcgtattcaa ggagaaattc gcgcaccggc
     5401 tcgtcgaagc gcagaacgcc aagtcgttcg gccagcgtat ggctgccttc aagggctttt
     5461 cacccgccga gcttgcgccg cagccgccta gcgtcagcga gccacgcacg ggcctatcca
     5521 tggggcagca taccgagctg atggcgcagg agtggcatat ttcccggagc gagcaggacc
     5581 ggttcgcgct ggaaagtcat cgcaaggccg ccgaagccta tcgctcgggc tatctggacg
     5641 acctcgtcgt gccgtttgcc ggcgtttttc gcgacaataa tttgcgggag gatgccagcc
     5701 tcgaccggct ggccacgctg aagccggcct tcgataaatc cgaacgcggt acattgacgg
     5761 ccgccaactc gacgccgctg accgacggag cggcttgcgt cctattggct tccgagacct
     5821 gggcggccag ccgcgcgctg ccgatcgagg cctatctgac gcacgcccag acggcggcca
     5881 ataacttcgt cgccggcgac ggcctgctgc tggcgccgac catcgcggtg tcgcgcttgc
     5941 tcgagcgcgc ccagctcacc ttgcaggatt tcgattatta cgagatccac gaagcattcg
     6001 ccgcacaggt gctcgtcacg ctgaaagcgt tcgaggatgc ggcctattgc aaaagcgttc
     6061 tcggtcgcga cgccccgctg ggttcgatcg atcgcgccaa actcaacgtc aagggctcga
     6121 gcctggcctt cggccaccca tttgccgcca cgggcgcccg catcattgcc aatcttggca
     6181 aacttctcgc cgtcaacggc ggccgcggcc taatttcagt gtgcacggcc ggcggcatgg
     6241 gcgttgcggc catcatggaa agcgcgcggg cggcagccct gcaggccgcc gcctgacggc
     6301 gcgcccgagt gccgagcgcg ccgcttttgc cgccgaggct gagcccacga gatgcctcgg
     6361 ctcaagacgt tggtggctta gcggccttgg tcggtacagc tgaagattgg ccgaccgccc
     6421 tggccactat cgacccagcc gacgacctta caatcagccg cgctggccga cgcggccatt
     6481 cccgccaaca gcgatagacc gagcagagct cccacaatcg ttctcatgat catctcctca
     6541 cagcattgtc gcagctcatt atcttt
//
LOCUS       DASTMT010000299         3459 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_3115672, whole genome shotgun sequence.
ACCESSION   DASTMT010000299 DASTMT010000000
VERSION     DASTMT010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3459)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3459)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3459
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3115672"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..212)
                     /locus_tag="VFR00_06880"
     CDS             complement(<1..212)
                     /locus_tag="VFR00_06880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009337335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HEU0159017.1"
                     /translation="MTPSLRLGVTGFARSGKTVFITALVRNLIAGGRLPFFAAMAQGR
                     IRRAYLEPQPDDEIPRFAYEEHLSELA"
     gene            complement(407..1288)
                     /locus_tag="VFR00_06885"
     CDS             complement(407..1288)
                     /locus_tag="VFR00_06885"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HEU0159018.1"
                     /translation="MAEHVAHPPRSERFIKAYLFVWGLLAAGGLTYLATLVWQPDWLS
                     PPPRRPQITAEAEPDSALRLASRAIAEVRDVRRSVGDVQRSLGEIRDTLDRSVGEDKV
                     VQTRLSALEGRVSDMSQPAAAPVATAPAVAGKGKTAERPQPGKSPAATKAAEKTEPGA
                     AATRVMSAVMSVPDQAEKPAPEAAPPIETGSIAPATITFGEPVVTRSNTNFAVQLGAG
                     RSMEALKLSWSLLLDQHGDALSALQPRVIAPRTEGGSYRLVAGPFDSRAEAEKVCAAM
                     ALSRNKCFSTVFMGEPL"
     gene            1592..2947
                     /gene="glmU"
                     /locus_tag="VFR00_06890"
     CDS             1592..2947
                     /gene="glmU"
                     /locus_tag="VFR00_06890"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018181530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEU0159019.1"
                     /translation="MPQPPLLIVVLAAGMGVRMRSRRPKVLHQLAGRSLLGHALARAQ
                     AGGADQIAVVVGPGMDAVRAEVLAHAATAEVFEQARQVGTADAVLAARPALERHRGDV
                     LVLFADTPLIETATIEALTATLAQGANIAVLGFNPEDPTGYGRLLVDARGRVTAIREH
                     KEASADERAQRLCNAGAMAFRVPSFVDLMARIGNANAAKEYYLTDAVALASSEGLIAL
                     PVVCSPQEAMGINTREQLAMAEAIFQARARAKAMREGVTMVAPETVWLSYDTAFGRDV
                     TIEPNVFFGPGVVVADGAEIGANSHIVGARIGEGARVGPFARLRPGADVGPHVHIGNF
                     VEVKNASLEAGAKANHLAYIGDGRVGAGANIGAGAIFCNYDGFNKHFTDVGKGAFIGS
                     NAALVAPVKIGDGAYIASGSVISRNVEADALAIARGSQELRPGWAAKFRALMQSRKKR
                     P"
     gene            3107..>3459
                     /locus_tag="VFR00_06895"
     CDS             3107..>3459
                     /locus_tag="VFR00_06895"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014495162.1"
                     /note="Catalyzes the first step in hexosamine metabolism,
                     converting fructose-6P into glucosamine-6P using glutamine
                     as a nitrogen source; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate
                     aminotransferase"
                     /protein_id="HEU0159020.1"
                     /translation="MCGIVGILGQRPVAHDLVDALRRLEYRGYDSAGVATVENGHLAR
                     RRAEGKLKNLEQRLAQAPLSGHAGIGHTRWATHGMPVERNAHPHMNKRVAVVHNGIIE
                     NFQELRQELSGKGHV"
BASE COUNT          571 a         1138 c         1180 g          570 t
ORIGIN      
        1 gcaagttcgc tcaaatgctc ttcgtaggca aagcgcggga tctcgtcgtc gggctgcggc
       61 tccaggtagg cgcgacggat gcggccctgc gccatcgccg cgaagaacgg caggcgtccg
      121 ccggcgatga ggttgcgcac cagggcggtg atgaagacgg tcttgcccga gcgcgcaaaa
      181 cccgtaacgc cgagccggag gctcggcgtc atcaggtcgg tgacgaaggc gccggcgctt
      241 ctgacagcgt cggccgtcgt gtcgctgagg tcggagagcc gcacgtgccg tccttgcgtt
      301 gccgcctgct agctaggacg gcggaacgat gacggcaaga cgccggcaac aagatgcctt
      361 gccgcgcggc tcagcagagg tctgctggag acccgaatgt ctccgcttac agcggttcac
      421 ccataaacac ggtggaaaag cacttgttgc ggctaagcgc catggcggcg cagaccttct
      481 cggcctcggc gcggctatca aacggacccg ccaccaggcg ataggagccg ccctccgtgc
      541 gcggcgcaat cacgcgcggc tgcaacgccg acagggcatc gccgtgctga tcgaggagca
      601 ggctccagct cagcttcagg gcctccatgg agcgcccggc gcccagctgt acggcaaagt
      661 tcgtgtttga gcgcgtcacc accggctccc cgaaggtaat ggtggccggc gcgatgctgc
      721 ccgtctcgat cggcggcgcc gcctcggggg cgggcttttc cgcctggtcc ggcaccgaca
      781 tgacggccga catgacgcgg gtggccgccg ccccgggctc cgtcttttcg gcggccttcg
      841 tcgccgccgg gctcttgccg ggctgcggcc gctcggcggt cttgcccttg ccggcgacgg
      901 ccggcgccgt tgccaccggt gcggccgctg gctggctcat gtcgctgacc cgtccctcga
      961 gcgcgctgag ccgcgtctgc acgaccttgt cttcgccgac gctgcgatcg agggtgtcgc
     1021 ggatttcacc caagctgcgc tggacgtccc ccaccgatcg gcgcacatcg cggacttcgg
     1081 cgatggcgcg gctcgccagg cgcaaggccg aatcgggctc ggcttcggcg gtgatctgcg
     1141 gtcggcgcgg cggcggtgac agccagtccg gctgccaaac cagggtcgca agataggtca
     1201 gcccgccggc ggccaacagg ccccagacga acagataggc cttgatgaag cgctcggacc
     1261 gcggggggtg cgcaacgtgt tctgccatag ccagcagcgt cctcgtgatg cccatgggtg
     1321 cgcgacttgg acgctgaaca gggcgccacc cgaatcggct acaaccgtgt caaatggcag
     1381 ggggattggt caacgcaccc aagcccgttg cgccgtccag cctgtggaca tgtgatagag
     1441 gtgccatagg gctctgttgc agataagacc caatgggagg cgagtgtcgt tggcgctata
     1501 cccgaagaca ccgcgcttag acagcaagcg tcgcgtgagc cctgcctgca gatgcgcctg
     1561 agcacctgaa ccgcgagact tagacaccgc gatgccgcaa ccgccgctcc tgatcgtggt
     1621 ccttgccgcc ggcatgggcg tgcgcatgcg ctcccgccgg cccaaggtgc tgcatcaact
     1681 ggcggggcgg tcgttgctgg gacacgcctt ggcccgcgcc caggccggcg gcgccgatca
     1741 gatcgccgtc gtggtcggcc ccggcatgga tgccgtccgc gcggaggtgc tggcgcacgc
     1801 cgcaacggcg gaggttttcg aacaggcacg gcaggttggg accgccgatg ccgtgctggc
     1861 tgcgcgcccg gcgctggagc gccatcgtgg cgacgtcctg gtgctgtttg ccgatacacc
     1921 cctgatcgag acggcgacca tcgaggccct gaccgccacc ctggcgcagg gggccaacat
     1981 cgccgttttg ggctttaacc cggaggaccc gaccggctat ggccgcttgc tcgtcgatgc
     2041 ccgtggccgc gtcaccgcca tccgtgagca caaggaggct tccgccgacg agcgtgccca
     2101 gcgcctgtgc aacgccggcg ccatggcttt ccgcgttccc tccttcgtcg acctcatggc
     2161 ccgcatcggc aacgccaacg cggccaagga atattacctg accgatgcgg tcgcccttgc
     2221 cagcagcgag ggcctgattg cgctgcccgt cgtctgttcg ccacaggagg cgatgggcat
     2281 caatacgcgc gaacagttgg ccatggcgga agccattttc caggcgcgcg cccgggcgaa
     2341 ggccatgcgc gagggggtga ccatggtcgc tcccgagacg gtttggctca gctatgacac
     2401 cgccttcggc cgcgacgtca ccatcgagcc caatgtgttc ttcgggcctg gcgtcgtggt
     2461 tgccgatggt gccgagattg gcgccaatag ccacattgtg ggggcgcgca tcggcgaggg
     2521 cgcccgggtt ggcccctttg ctcgcctgcg cccgggtgcc gacgtcggcc ctcacgtcca
     2581 catcggcaat ttcgttgagg ttaagaacgc cagcctggaa gccggtgcca aggccaacca
     2641 tctggcctac atcggcgacg ggcgggtagg tgcgggtgcc aacatcggcg ccggcgcgat
     2701 tttttgtaac tatgatggct tcaacaagca tttcaccgac gtcggcaagg gcgccttcat
     2761 cggctcgaac gccgccctcg tcgcccccgt caagatcggc gatggcgcct atatcgcctc
     2821 gggcagcgtc atttcccgca acgtggaggc cgacgccttg gccatcgctc gcggcagcca
     2881 ggagctgcgg ccgggttggg ccgccaaatt ccgggcgctg atgcaaagcc gcaaaaaacg
     2941 accctgactt cgggcgcggg ctcgcgcacg cgcagccagg cgcggcaatt gagagtaatt
     3001 cgtctcaatt gaacgtgaaa aagcaagaaa cattgtttgt cttgagcgta ttgggaggat
     3061 cggactaggt aggttgaaag caagccttga ggcgtgaggc acacacatgt gcggcattgt
     3121 tggaattttg ggtcagcggc cggtggcgca cgatttggtc gatgcgctgc gtcggctcga
     3181 gtaccgcggc tatgattcgg ccggcgttgc caccgtggaa aacggccacc tggcgcgccg
     3241 gcgcgcggag ggcaagctca agaacctgga gcagcggctc gcccaagcac ccctgagcgg
     3301 gcatgccggc atcggccaca cgcgctgggc aacgcacggc atgcccgtcg agcgcaatgc
     3361 ccatccgcat atgaacaagc gggtggcggt ggtccacaac ggcatcatcg agaacttcca
     3421 ggagctgcgc caggagctga gcggcaaggg gcacgtgtt
//
LOCUS       DASTMT010000300         6666 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_2456567, whole genome shotgun sequence.
ACCESSION   DASTMT010000300 DASTMT010000000
VERSION     DASTMT010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 6666)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6666)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6666
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2456567"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2024)
                     /locus_tag="VFR00_06900"
     CDS             complement(<1..2024)
                     /locus_tag="VFR00_06900"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HEU0159021.1"
                     /translation="MAIVETEKKGEIAVIRMDNPPVNALGHALRVGLERAFAAANADA
                     GVKAIVLTGTGKFFSAGADISEFKAQMKEPFLPQLIDKIEQGSKPVVAALNGTALGGG
                     LELALGCHYRVASKDVRQLGLPEIKLGILPGAGGTQRLPRAIGVEPALQLITTGSFID
                     AQKAAAAGLVDKVVEQDLLGAAIAFAEAQAGKPPRRIGDKKIEPGTFAADAFHKAKMQ
                     IGRHPSGPLAAKAAIAAVEAATELPLAEGNQRERTLFRELAASPYARALQYAFFAERQ
                     AANLPGIGGDTKLREVNTVGILGAGTMGTGIALAFLNGGFPVTIVETTQEALDKGVAR
                     IKDTLSANAKRGRITAAQAADRLAKLTPSLELADLGPADLIIEAVFENMALKKEVFGK
                     LDKIAKSGAIIATNTSTLDVDAIAAETKRPQDVVGLHFFSPANIMRLLEIVRAEKTAG
                     DVMATAMAIAKRIGKVGVQAGVCDGFVGNRMLAAYGGEVQAMNLEGAMPQEIDGALEG
                     WGMAMGPLAVSDLAGLDVGYRIRKERKLTGEAAQNARIPDKIVEMGRHGQKTGAGYYK
                     YDENRKRVVDPEIEALIRGEAEALQIKQRPIAADEIVERSLLRLANEGAKILEEGIAL
                     RASDIDTMYLNGYGFPAWRGGPMWQVDNVIGVETAAQRIKAYEAKYGARW"
     gene            2162..3802
                     /locus_tag="VFR00_06905"
     CDS             2162..3802
                     /locus_tag="VFR00_06905"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009467773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase"
                     /protein_id="HEU0159022.1"
                     /translation="MLRGLMMDRPLLVSSVIDYAAEVFPDVEIVSQTIEGGLHRYGYA
                     QARRRIARLANALLRLGVREGDRVATLAWNGYRHFELYFAISGIGAVCHTINPRLFPE
                     QISYIANHAGDSVLFFDLTFLPLVEKLAPSCRSINTFVLMTDQEHMPRSGPLQNLLCY
                     EDLLAQAEDQFSWPEFDEHAACALCYTSGTTGEPKGVLYSNRSMLLHTFFLISTRPDL
                     FAHNRTILPVVPLFHANAWSLPYVTPLTGCPLILPGARLDGPTLFDLMEAEGVEAGWG
                     VPTVWLGLLEEFRKRGRKPKKLVQILSGGSAVPQAMIDTFTRDWGIEVTHGWGMTEMS
                     PVGTLTVFRPDERAKSPVERAALSARQGRRMFGVDLKVIDEAGRRAAADGQTSGELFV
                     RGATVVSGYFNNPEASAKQIDDEGWFGTGDVAKLTPDGWLLIVDRTKDLVKSGGEWIS
                     SIDVENAALAAVGGIANCAVIGVPHPKWNERPLLVVVKAPGAEPSKAEILDALGAKIA
                     KWQMPDDVVFVDALPMTATGKISKKDLRAKFADYQLPD"
     gene            3985..4605
                     /locus_tag="VFR00_06910"
     CDS             3985..4605
                     /locus_tag="VFR00_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013420465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEU0159023.1"
                     /translation="MLRPNLSRIAALAAVMLITAALAGCGINNIPTFEQNAKAKWSEV
                     LSQYKRRADLVPNLVETVKGFAAQEQQVLTSVIEARAKATQAQVQLPADAVNNPEAMK
                     RFQDAQNQLSGALGRLLAVVERYPDIKSGQNFLALQSQLEGTENRIAIARRDYIEAVR
                     IYNTELTTFPGRIWRSVMYASAQPMATFDITPQEQQTPRVDFGTKK"
     gene            4776..5606
                     /locus_tag="VFR00_06915"
     CDS             4776..5606
                     /locus_tag="VFR00_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HEU0159024.1"
                     /translation="MPAPRRRALAGAAAAAVRLALALVLLASSLGLAAAAPKFPALSG
                     RIVDEANLLSAEDRRALEAELQALEEKSSDQLVIYTARSLQGYEIEDFGYQLGRAWQI
                     GQKGQDNGIVLIVAPNERKVRIEVGRGLEPQLTDVMSKLIVENAILPAFRRGDFAAGI
                     KAGVRDIRDVVLGDAEAVKARARAGAKREGGGSGLSPALLFLIFFIVMALVLVQADAR
                     QTAHPQRVGGRNRRASPGPIIIPGSWGGGSGSWGGSGGGGGFSGGGGSFGGGGASGSW
                     "
     gene            5635..6255
                     /locus_tag="VFR00_06920"
     CDS             5635..6255
                     /locus_tag="VFR00_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HEU0159025.1"
                     /translation="MTIVSAADRKRVAEAINAAERQTSGEIVAVIAGESASYAYAPFL
                     WAALVALAVPFPLIFWTWWPIQHIYILQLVVFIALLLLFMYRPLRLSLVSQRVKKERA
                     HRRAVEQFLAQNLHTTPSRTGVLIFVSVAERFAEIIADAGINEKVPEGTWQVIIDRLT
                     KDIGDGQAVDGFVRAVEAVGGELARHFPPGSHDPNAFANHLIVLPS"
     gene            6464..>6666
                     /locus_tag="VFR00_06925"
     CDS             6464..>6666
                     /locus_tag="VFR00_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016843589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase type II"
                     /protein_id="HEU0159026.1"
                     /translation="MDGPQLLKREIKVLVFDQYGTVVDMQKGLTEAVGPFLAKKGWSG
                     EANSFVTWWRRTHFENSMIDALC"
BASE COUNT         1116 a         2193 c         2153 g         1204 t
ORIGIN      
        1 ttccagcgcg cgccgtattt tgcctcgtag gccttgatgc gttgggccgc tgtctcgacg
       61 ccgataacat tgtccacctg ccacatcggg ccgccgcgcc aggccgggaa gccgtagcca
      121 ttgagataca tggtgtcgat atcgctggcc ctgagcgcga tgccttcctc gaggatcttg
      181 gccccctcat tggcaaggcg cagcaggctg cgctcgacga tttcatcggc ggcaatcggc
      241 cgctgcttga tttgcaacgc ctcggcctcg ccgcgaatga gcgcctcgat ctccgggtca
      301 accacgcgtt tgcggttctc gtcatacttg tagtagcccg ccccggtttt ttgcccgtgc
      361 cgccccattt ccacgatctt gtccgggatg cgcgcattct gggcggcttc accagtcaac
      421 ttgcgctcct tgcggatgcg atagccaacg tcgagcccgg cgagatcact caccgccagg
      481 ggacccatcg ccatgcccca gccttcgagt gcgccgtcga tctcctgcgg catggcacct
      541 tcgaggttca tggcttggac ttctccgccg taggccgcca gcatgcgatt gcctacaaag
      601 ccgtcgcaca cgccggcctg cacaccgacc ttgccgatcc gcttggcgat cgccatggcc
      661 gttgccatca cgtcgcctgc ggtcttttcg gcgcgcacga tctccaacag ccgcatgatg
      721 ttggcgggcg agaagaagtg aaggccaacc acgtcctgcg gccgcttggt ctcagccgcg
      781 atcgcatcca cgtccaaggt ggaggtgttg gtggcgataa tggcgccgga tttggcgatc
      841 ttatcgagct tgccgaacac ctctttcttg agcgccatgt tttcgaagac cgcctcgatg
      901 atcaggtccg ccggtccgag gtccgccagc tcgagggacg gcgtcagctt ggccaagcga
      961 tcggccgcct gggccgcggt aatgcgcccg cgcttggcgt tggccgagag cgtatccttg
     1021 atgcgcgcca cccccttatc gagtgcctcc tgcgtggttt ccacgatggt gacgggaaag
     1081 ccgccgttga ggaaggcgag cgcgatgccg gttcccatgg tgccggcgcc gaggatgccg
     1141 accgtgttga cctcgcgcag cttcgtgtcg ccgccgatgc ccggcaggtt ggccgcctgc
     1201 cgctcggcga agaaggcgta ttgcagggca cgcgcgtagg ggctggcggc gagctcgcga
     1261 aagagggtgc gctcgcgttg gttaccttcg gcaagcggca gctcggtcgc cgcctcgacc
     1321 gcggcgatcg cggctttggc cgccaagggc ccgctcgggt ggcggccaat ctgcattttc
     1381 gccttgtgga aggcgtcggc ggcaaacgtc ccgggctcga tcttcttgtc gccgatgcgg
     1441 cgcgggggct tgcccgcctg cgcttcggca aaagcaatcg ccgcgcccaa gaggtcttgc
     1501 tcgaccacct tgtcgacgag gcccgccgcg gccgccttct gggcatcgat gaagctaccc
     1561 gtggtgatca gctgcagggc cggctcaacg ccgatggcgc gcggcaggcg ctgggtaccg
     1621 ccggcacccg gaaggatgcc gagcttgatc tccggcaaac cgagctggcg cacgtccttg
     1681 ctggcgacgc gatagtgaca gccgagcgcg agctccaggc caccgcccaa ggccgtgccg
     1741 ttgagcgcgg caaccacggg tttgctaccc tgctcgatct tgtcgatcag ctgcggcagg
     1801 aacggctcct tcatttgcgc cttgaattcg ctgatatcgg cgccggccga gaagaattta
     1861 cccgtgcccg tcagcacgat ggccttgacc cccgcatcgg cattggcggc ggcaaaggcc
     1921 cgctcgaggc ccacccggag cgcgtggccc agcgcgttga ccgggggatt gtccatgcga
     1981 atgaccgcga tctcaccctt tttctcggtc tccacgatcg ccacgacgca ctctcctctg
     2041 gttggcctct gatggcttgg cggctcggca tgcctggcat cgacccggct tggcgctggg
     2101 ctggcgccga ggttactctc accgaaccaa gcataccaga agcataggag gaggaacgcc
     2161 catgctgcgc ggattaatga tggaccgccc gctcctggtt tccagcgtga tcgattacgc
     2221 agccgaggtg tttcccgacg tcgagatcgt ctctcagacc atcgagggcg ggcttcaccg
     2281 ctacggctac gcccaggcgc gccggcgcat cgcccggctg gccaatgccc tgttgcggct
     2341 tggcgtcagg gaaggcgacc gcgtcgccac cctggcctgg aacggctatc gccacttcga
     2401 gctctatttt gccatctcgg ggatcggcgc ggtctgccac accatcaatc cgcgcctgtt
     2461 cccggagcag atctcctata tcgccaacca tgccggcgac agcgtactgt tcttcgatct
     2521 gacgtttctg ccgctggtcg aaaaactggc gcccagttgc cgcagcatca acacctttgt
     2581 gttgatgacc gaccaagagc acatgccccg cagcgggccc ttgcagaacc tgctgtgcta
     2641 cgaggacttg ctggcccagg cggaggacca gttcagctgg ccggaattcg acgagcacgc
     2701 cgcttgcgcg ctctgttaca cctcgggcac caccggcgag ccgaaggggg tgctctactc
     2761 caatcgctcc atgctgctgc acaccttttt tctgatttcc acgcgtcccg acctattcgc
     2821 gcacaatcgc accattctgc ccgtggtgcc gctgtttcac gccaatgcct ggagcctgcc
     2881 ctacgtcacg ccgctgaccg gctgtcccct gatcttgccc ggcgcccggc tcgacggccc
     2941 gaccctgttt gatctgatgg aggcggaagg cgtcgaagcg ggatggggcg tgccaaccgt
     3001 ctggctcggc ctgctcgagg aattcaggaa gcgcggccgc aagcccaaga agctcgtgca
     3061 gatcctgagc ggcggctcgg cggtaccgca agccatgatc gacaccttca cgcgcgactg
     3121 gggcatcgag gtgacgcatg gctggggtat gacggagatg tctccggtcg gcacgctcac
     3181 ggtttttcgg cccgacgagc gggcaaagtc gccggtggag cgggccgcgc ttagcgcccg
     3241 acagggccgt cgcatgtttg gcgtcgacct caaggtgatc gacgaagcgg gccggcgcgc
     3301 agccgccgac gggcagacat cgggggagct gttcgtgcgc ggcgccaccg tggtatcggg
     3361 ctatttcaac aacccggagg cttccgccaa gcaaatcgac gacgagggct ggtttggcac
     3421 cggcgacgtg gcgaaactga cgcccgacgg ctggctcttg atcgtcgatc gcaccaaaga
     3481 cctggttaag tccggcggcg aatggatcag ttcgatcgac gtggagaatg ccgccctggc
     3541 cgccgtcggc ggcatcgcca attgcgcagt gatcggcgtg ccgcacccca aatggaacga
     3601 gcgtccgctg ctggtggttg tgaaggcgcc cggcgccgag ccgagcaagg ccgaaatcct
     3661 cgacgcgctc ggggcgaaaa tcgccaagtg gcagatgccc gatgacgtgg ttttcgtgga
     3721 cgccctgccg atgacggcga cgggcaaaat ctccaaaaaa gatctgcgcg ccaagtttgc
     3781 cgactaccag ctgcccgact gaggcaagcg cgtctggccg tttacgttgc tttcccgcgg
     3841 gcagcacggc cgtttgccgt cgctgccaag gaattgccag gtgccgggcg ggctgcctct
     3901 ggcgccggcg gtatcggcgc accattttca ctgcaccgag aaacgaggcc ggccgcgggg
     3961 ccgcccctgc cttgaggaaa gcacatgttg cgtccaaacc tcagtcgcat cgctgcgctc
     4021 gcagccgtca tgctcatcac cgccgcgctg gcgggctgtg gcattaacaa tattccgacc
     4081 ttcgaacaga acgccaaggc caaatggtcg gaggtgctga gccaatacaa gcggcgcgcc
     4141 gacctggtac ccaacctggt cgaaaccgtg aaaggcttcg ccgcccaaga gcagcaggtt
     4201 ttgacctccg tgatcgaggc gcgcgccaag gcgacgcagg cacaggtgca attgcctgcc
     4261 gatgcggtca acaatcccga agccatgaag cgcttccagg acgcgcagaa ccagctcagc
     4321 ggcgcccttg gtcgcctgct cgccgtcgtc gagcgctatc ccgacatcaa atccggccag
     4381 aacttccttg ccctgcagtc gcagctcgag ggcacggaga accgcatcgc catcgcgcgg
     4441 cgcgactaca tcgaggcggt gcgcatctat aataccgagc tcaccacctt ccctggtcgg
     4501 atctggcgct cagtgatgta tgcctcggcc cagccgatgg cgaccttcga catcaccccg
     4561 caggagcaac aaaccccgcg cgtcgacttc gggaccaaga aataaccggg cgtgcgccgg
     4621 acgcgccggg cacgggcgcc agcgccccgc gcacgggatc gcgagcgccg aaacggagcg
     4681 cgccccccga gcgccattgt cgaggagacg cgggactggg gatgtcgagc gtggctgcca
     4741 aagccttaac ccctgccggc tgtttcggcc cccgcctgcc ggcgccgcgg cggcgagccc
     4801 tcgccggcgc tgccgccgcg gccgtgcgcc tggcgctggc gctcgtcctg ctcgccagca
     4861 gccttggctt ggcggcggcg gcgccgaagt ttccagccct gagcggccgc attgtcgatg
     4921 aggccaacct gttgtcggcg gaggaccgcc gtgcgctgga agcagagttg caggcgctcg
     4981 aagagaaatc gtccgatcag ctcgtgattt acacggcgcg ctcgctgcag ggctacgaga
     5041 tcgaggattt tggctaccag ctcggccgcg cctggcagat cgggcagaag ggccaggaca
     5101 acggcatcgt tctgatcgtg gcgccgaacg agcgcaaggt gcgcatcgag gtcggacgcg
     5161 ggctcgagcc gcagctcacc gacgtcatga gtaagctcat cgtcgagaat gcgatcctgc
     5221 ccgcattcag gcgcggcgac ttcgccgcag gcatcaaggc cggcgtacgc gacatccgtg
     5281 atgtcgtgct cggcgacgcc gaagcggtga aggcgcgggc ccgcgccggg gccaagcgcg
     5341 agggtggtgg ctccggtctt tcgccggcgc tgctgtttct catctttttc atcgtgatgg
     5401 cgctggtgct cgtgcaagcc gacgcgcgcc agaccgccca tccccagcgc gtcggcgggc
     5461 gcaatcggcg cgcgtcgccc gggccgatca tcattccggg ctcctggggc ggcggctccg
     5521 ggagttgggg cggctcgggc ggcggcggtg gcttctcggg cggcggcggc tcgttcggcg
     5581 gcgggggcgc gtctggcagt tggtagggtt gaagcaaggc acgggatttc ggtcatgacg
     5641 atcgtcagtg cagcggatcg caagcgcgtg gccgaggcca tcaacgctgc cgaacgccaa
     5701 acttcgggcg agatcgtggc ggtgattgcg ggcgagagtg cgagttacgc ttacgctccc
     5761 tttctgtggg ccgcgttggt cgcgctggcg gtaccgtttc ccctgatctt ctggacctgg
     5821 tggccaatcc agcacatcta cattctgcag ctcgtcgtct tcatcgctct ccttcttctc
     5881 ttcatgtacc gtccgttgcg gctgtcactc gtgtcgcagc gggtgaagaa ggagcgcgcg
     5941 catcgacgcg ccgtcgagca attcttggca caaaacctcc acaccacccc gagccgcacc
     6001 ggcgtcttga tttttgtctc ggtggcagaa cggttcgccg aaatcatcgc cgatgccggc
     6061 atcaacgaaa aagtgccaga agggacctgg caggtgatca tcgaccggct caccaaggac
     6121 atcggcgacg gccaggccgt ggacggtttt gtgcgcgcgg tcgaggcggt cggtggcgag
     6181 ctcgcgcgcc attttccgcc gggcagtcat gatcccaacg cctttgccaa ccatctgatc
     6241 gtgctgccct cctaaaaagt cgcggcacgg tgaactgcaa cggcgtcctt gccgcgatgc
     6301 ctcgaggccg cggcacgaca ggccatcgcc gaggcttgca tccgacttcg aggcaaaaag
     6361 acttggcaag gggcccgaca ctcggcttta tcgaccgaat tggcccatag ccttgcgcct
     6421 aaactcgggc tgggcgcaac gccaaagcag gcaagaggaa cgcatggacg gtccgcaact
     6481 actcaagcgc gaaatcaagg tgctggtgtt cgatcaatac ggcaccgttg tcgacatgca
     6541 gaagggcttg acggaagcgg tcgggccatt tctcgcgaaa aaaggctgga gtggagaagc
     6601 aaacagcttc gtcacctggt ggcggcgcac gcacttcgaa aattccatga tcgatgcctt
     6661 gtgcga
//
LOCUS       DASTMT010000301        12016 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_3446212, whole genome shotgun sequence.
ACCESSION   DASTMT010000301 DASTMT010000000
VERSION     DASTMT010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 12016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12016
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3446212"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            124..1041
                     /locus_tag="VFR00_06930"
     CDS             124..1041
                     /locus_tag="VFR00_06930"
                     /EC_number="3.8.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014496277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018786 - haloalkane dehalogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloalkane dehalogenase"
                     /protein_id="HEU0159027.1"
                     /translation="MADGSQPYAEKRFVDVKGRRMAVIDEGEGKAIVFQHGNPTSSYL
                     WRNVMPHCRGLGRLVACDLIGMGDSDKLAPPGPQRYGYAEQREYLFALWDKLDLGNEI
                     VFVLHDWGSALGFDWAAAHPERVAGIAYMEALVTPLTWEDWPAHARGAFQGFRSPAGE
                     DLILAKNMFVERILPGSILRQLSEAEMAHYRRPFAVAGEDRRATLSWPRQIPIDGAPA
                     EVVRTVESYARWLAASPVAKLFINAEPGSILVGRQREICRAWPNQTEVTVAGLHFVQE
                     DSPVAIGEAVAAFVRRLRSDQRRPAAPAR"
     gene            1229..2836
                     /locus_tag="VFR00_06935"
     CDS             1229..2836
                     /locus_tag="VFR00_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013896402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HEU0159028.1"
                     /translation="MPKLQSAISTNSEEFKANAAAMQALLADLAAKRTEAALGGPERL
                     RERHTARGKLLPRDRVLRLIDAGSPFLELSPLAGYDLYSKDIHAGGIITGVGRIAGRE
                     CVIVCNDATIKGGTYFPITVKKHLRAQEIARENRLPCIYLVDSGGANLPQHTEVFPDR
                     EHFGRIFYNQATLSSLGIPQIACVMGSCTAGGAYVPAMSDESIIVRKQGTIFLGGPPL
                     VKAATGETVSAEELGGAEVHTRQSGVADHYALNDEHALEIVRSIVANLNSAKQVDITL
                     REPRPPAYDPAELDGVVPASLMTQYDTREIIARLVDASELDEFKTNYAQTLVTGFAHI
                     EGLPVAILANNGILYSETALKATHFIELACQRRIPLLFLQNITGFMVGRAAEAGGIAK
                     DGAKMVTAVACAQVPKVTVIIGGSYGAGNYAMCGRAYSPRFLFTWPNARISVMGGAQA
                     AGVLATVRRENIEAERGTWSAEEEAAFKAPILQNFEREGHPYYATARLWDDGIITPGE
                     TRRVVALALSASLNAPIPPPRFGVFRM"
     gene            2894..4078
                     /locus_tag="VFR00_06940"
     CDS             2894..4078
                     /locus_tag="VFR00_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018457338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase/NADH oxidase"
                     /protein_id="HEU0159029.1"
                     /translation="MTEPLLFTPLALKSVTLKNRVVVAPMHQYAAVRGHATDWHLVNA
                     GRYAQGGAGLVIMESTKIARNGCGTVGDVGLWADAFIPPLARCAQFIKAHGAVAGLQL
                     GHSGRKARLTRPWEGGKPLTGTEAEVFDWEAWDLVAPSAVPHTERSPIPRALSLQEVR
                     ELVAKWGAAAARAVKAGFDVLEIHAAHGYLVHQFLSPRANLRGDAYGGSELNRMRFAL
                     EVAEAVRAAWPNDKPLFMRLSCEDDAGWDLEQSIRLARLLKASGIDVIDCSSGGTLAH
                     SPMEGVRAKKYGYQVPYAERIRREAGIMTMAVGHIVHADQAEAILRENRADLVALARE
                     IMHNPSWPMDAAQKLGVDPQFSLVPPAYAYWLAKRAASGFEGTPSTWSKGLPPRGEPA
                     DA"
     gene            4243..5040
                     /locus_tag="VFR00_06945"
     CDS             4243..5040
                     /locus_tag="VFR00_06945"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HEU0159030.1"
                     /translation="MPLYGSEALKRIRDGKLALGLGVNHLRGSAVPLLAKAAGYDWLF
                     IDAEHGAISTPEIAQIAMAALPMGVVPIVRICSGALDEGTRALDNGALGIVVPHVDTP
                     EQARELVEAFRFSPMGHRSTGGSNACFGYRPPSAAEAQKTLNAQILVVPMIETPRAVE
                     NASAIAAIEGIDVLLIGSNDLSLEMGIPGQVGHERVRAAYETVAAACRQHGKVLGMGG
                     VYDQEVASRYIGLGARMILGASDHSLLLEAATRRAEFLRSLAPQSAA"
     gene            complement(5078..5899)
                     /locus_tag="VFR00_06950"
     CDS             complement(5078..5899)
                     /locus_tag="VFR00_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011442538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEU0159031.1"
                     /translation="MPKLGSIRVGIGGWTFEPWRGVFYPKGLAHAKELAYASSKLTTI
                     EINGTFYRTQTPATFAKWRQEVPDGFVFAVKGPRYVVQKRVLAEAGEALAFFFKSGLG
                     ELGDRLGPILWQFAPSKKFDATDFAAFLALLPSELAGRKLRHAVEVRHPSFATPEFIA
                     LVRKYKTPIVFAEHDTYPAIADPVGDFVYARLQKGDDAIATGYPPRELDAWAKRAATW
                     AAGGQPADLPSLTAKVPKQAARDVFLYVIHEGKVRAPAAAMALIERLGARVRAAG"
     gene            6037..6471
                     /locus_tag="VFR00_06955"
     CDS             6037..6471
                     /locus_tag="VFR00_06955"
                     /inference="COORDINATES: protein motif:HMM:NF016701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HEU0159032.1"
                     /translation="MSITRGRCQCRAIVYEFEGPPKWVMHCHCADCRRAVSSALATYI
                     GVRRDQFRYLAGEPAAYASSEGVKRYFCRACGTPIAYTGANWPDEVHLLHGSLDDPSQ
                     WPPTGHAYVGEQIHWFDVADQLPRYLTTAGKGAKPVRNGPRT"
     gene            7177..7413
                     /locus_tag="VFR00_06960"
     CDS             7177..7413
                     /locus_tag="VFR00_06960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0159033.1"
                     /translation="MRLFTVLGLSLLALFALAAEAGATDKKKKAQRHASAPAAAATPR
                     PDRYRELLADKMPVGSSEWWEQMRREGRLGGETP"
     gene            7511..8359
                     /gene="kdsA"
                     /locus_tag="VFR00_06965"
     CDS             7511..8359
                     /gene="kdsA"
                     /locus_tag="VFR00_06965"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HEU0159034.1"
                     /translation="MPEPVKPNAAVAVGSVSFANDAPLALFAGPCQMESRQHALEMAS
                     ALKEICGRLGVGLVYKTSFDKANRTSLASKRGVGLDGALAVFAEIRASLGLPVVTDVH
                     EASQCARVAEVVDVLQIPAFLCRQTDLLVAAAETGRPVKVKKGQFLAPWDMRHVVAKV
                     TGSGNANVLVTERGASFGYNTLVVDMRALPILREIGCPVIFDATHAVQQPGGQGAQSG
                     GERRFVAPLARAAVAVGIAGLFIETHEDPDRAPSDGPNMVPLKDFEGLLGELMAIDRL
                     VKRRQA"
     gene            complement(8356..8979)
                     /locus_tag="VFR00_06970"
     CDS             complement(8356..8979)
                     /locus_tag="VFR00_06970"
                     /inference="COORDINATES: protein motif:HMM:TIGR02451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ChrR family anti-sigma-E factor"
                     /protein_id="HEU0159035.1"
                     /translation="MTITHHLGSETLMSFAAGTLPGALAAVAGAHIALCRRCRDELVW
                     FERIGGALLDDVAPLPLTTGVPALPAPGAPASSAPVGGNSANPVLRLEAQNFATVSWR
                     RIGLGLWHHPLPLTGTGTLQLIKAAPSASVPEHGHGGAELTLILRGALSDATGSYGQG
                     DVADLDQEIEHTPIADPKRGCVCAVANEVPTRFHGLLARLFQPWHGL"
     gene            complement(8976..9509)
                     /locus_tag="VFR00_06975"
     CDS             complement(8976..9509)
                     /locus_tag="VFR00_06975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU0159036.1"
                     /translation="MSELLARIAQESDAAAFHELYQSYGPRLKAYLVRRGADAATAED
                     LAQETLLAVWRRAGLFSSAKGSASTWIFSIARNLWIDRLRREVPWQELPAAHAEAASP
                     DPSPDTLLAENEVQVRVRAALAELPAEQQRVLELAYLQGLSHGEIAARLGVPLGTVKS
                     RVRMAYQRMRTAVEDLK"
     gene            9734..10645
                     /locus_tag="VFR00_06980"
     CDS             9734..10645
                     /locus_tag="VFR00_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013949624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEU0159037.1"
                     /translation="MAAARRIICVGHAALDRIYRIEAFPPEPSKVRALEHIEAGGGMA
                     ANAAVAIARLGGKAELWSRTGDDAAGATVRAGLKAERVDVRYLQAFEGARTSTSAIIV
                     DRHGERLIVGQRDAGMPSGTSWLPLERVREADAVLGDLRWLEALRSVFARARQEGVPT
                     VLDADLGAREALESILRLTDYAVFSAPALRELVPAGSDAERLESVLGFGARYAGVTLG
                     ADGYLWRDQEGTRRLPAFGVAVADTTGAGDAFHGALALLLAEGRPMHECARSASAVAA
                     MKCTRLGSRSGLPTRAELTAFLATHAA"
     gene            10778..11182
                     /locus_tag="VFR00_06985"
     CDS             10778..11182
                     /locus_tag="VFR00_06985"
                     /inference="COORDINATES: protein motif:HMM:NF020918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HEU0159038.1"
                     /translation="MADKNSSGRVENGFMPMPLPLGFEAFMEMQRPALTAMAQMNGKV
                     YDNLAAINRNWVTFVNRRLKEELAMPQNLAGCKTVQDMYGVYSEFFQTAVADYQSEFE
                     QMTKLGKSLAEDTAQFVQARIDDVAREGRNGR"
     gene            complement(11257..>12016)
                     /locus_tag="VFR00_06990"
     CDS             complement(11257..>12016)
                     /locus_tag="VFR00_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013418373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU0159039.1"
                     /translation="PEAFLPVARVNRQPLALLKGIDRAAEQLLDNTRRFAAGLPANNA
                     LLWGARGMGKSSLVKAAQAEVRRTLGLGRASGKPDLKLIEIHREDIAALPQCLAHLRT
                     AAHRFIVFCDDLSFDKDDTSYKSLKAVLEGGIEGRPPNVVFYATSNRRHLMPRDMMEN
                     ERSTAINPSEAVEEKVSLSDRFGLWLGFHNATQDEYLEMVRSYAAHHRLKLDADELKR
                     EALEWSMTRGARSGRVAWQFIQDLAGRLGHKLED"
BASE COUNT         1973 a         3855 c         4195 g         1993 t
ORIGIN      
        1 cccggccgtt cgcgcagccg cgggcgacct gccgcagcgg caacccgcag gcctcggggc
       61 gcagcacggc cgaccggcga aggcgcgcga cggccagctc tgtaaagcct ggggagacac
      121 ggcatggcag acggcagcca accctacgcg gagaagcgct tcgtcgacgt caagggtcgg
      181 cgcatggctg tcatcgacga aggcgagggc aaggccatcg tcttccagca cggcaatccc
      241 acctcctcct atctgtggcg caacgtcatg ccgcactgcc ggggtctcgg gcggctggtc
      301 gcctgcgacc tgatcggcat gggtgacagc gacaagctcg cgccaccggg gccacagcgc
      361 tacggctatg ccgaacagcg cgagtacctg ttcgcactct gggacaagct cgacctcggg
      421 aacgagatcg tctttgtcct gcatgattgg ggttcggcgc tcggcttcga ttgggctgcg
      481 gcgcaccccg agcgggtcgc cgggatcgcc tacatggaag cgctggtcac ccccttgacc
      541 tgggaggact ggccggcgca cgcgcgaggc gcctttcaag gctttcgctc gccggcgggc
      601 gaggatctga tcctcgccaa gaacatgttc gtcgagcgca ttttgccggg ttcgatccta
      661 cgccagctga gcgaagcgga gatggcacat taccgccgcc ccttcgccgt tgcgggcgag
      721 gaccggcgtg caacgctgag ctggccacgg cagatcccca tcgacggtgc gccggccgag
      781 gtggtgagga ctgtcgagag ctatgcgcgc tggctggcgg ccagtcccgt cgcgaagctc
      841 ttcatcaatg ccgagccggg ctcgatcctg gtgggacgtc agcgcgaaat ctgccgcgcc
      901 tggccgaacc agaccgaggt gacggtcgca ggcctgcact ttgtgcagga ggatagtccc
      961 gtcgcgatcg gcgaggcggt cgcagccttc gtgcggcggc tgcggtcgga ccagcgccgg
     1021 cccgccgcgc cggcgcggtg atggcttccg ccgccttcca agggtagaac gtgatgctga
     1081 gtggcgcgtc ggtcggccgc ggcaagcgcg cttgcgcggc gttggcaggc gttgcccggg
     1141 aggccggccg caaaaaatcg gggctgaacg tgccggctgc ccgcggcaaa gggcaaggca
     1201 aacggccggc agggaagcag gagagcagat gccgaaactg cagtccgcca tctcgacaaa
     1261 ctccgaggag ttcaaggcga acgcggccgc catgcaggcc ttgctggccg accttgccgc
     1321 caagcggacc gaggcagcgc tgggcgggcc agagcgcttg cgcgagcggc acacggcgcg
     1381 cggcaagctg ttgcctcgcg accgggtgtt gcgcctcatc gatgcgggct cgccgtttct
     1441 cgagctgtcg cccttggccg gttacgatct ttatagcaag gacatccacg ccggcggcat
     1501 cattacgggc gtgggacgca ttgcgggacg tgaatgcgtg atcgtgtgca atgatgccac
     1561 catcaaaggc ggtacctact ttccgatcac ggttaagaag cacctgcgcg cccaggagat
     1621 cgcgcgcgag aaccgcctgc cgtgtattta tttggtcgat agcggcggtg ccaacctgcc
     1681 gcagcacacc gaggtgtttc cggatcgcga gcacttcggg cgcatttttt acaatcaagc
     1741 gacgctgtcg tcgctgggca ttccgcagat tgcctgcgtt atgggctcgt gcacggccgg
     1801 tggggcttac gttcctgcca tgtcggatga gtcgatcatc gtgcgcaaac agggcacgat
     1861 ttttctcggc gggccgccgc tggtgaaggc cgccaccggc gagacggtga gcgcggagga
     1921 gttgggcggc gccgaagtgc acacgcgcca gtcgggtgtg gccgatcatt acgccctcaa
     1981 cgacgagcat gcgctcgaga tcgtgcgcag catcgttgcc aacctgaata gcgccaagca
     2041 ggtggacatc accctgcgcg agccgcgacc gccggcctat gaccccgccg agctcgacgg
     2101 tgtcgtgccg gcttcattga tgacgcagta cgacacgcgc gagatcatcg cgcgcctggt
     2161 cgacgcctcc gagctcgatg aattcaagac caactatgcg cagaccctgg tgacgggctt
     2221 cgcgcacatc gaagggctgc cggtggcgat cctcgccaac aatggcattc tctattcaga
     2281 gacggcgctg aaagccaccc acttcatcga gcttgcctgc cagcgccgca tcccgctgct
     2341 gttcttgcag aacatcacgg ggttcatggt gggccgggcg gcggaggctg ggggcatcgc
     2401 caaggatggt gccaagatgg tgaccgcggt ggcgtgcgcg caggtcccga aagtgacggt
     2461 gatcatcggc ggctcgtatg gcgccggcaa ctacgccatg tgcgggcggg cctactcgcc
     2521 acggtttttg ttcacctggc ccaacgcgcg catctcggtg atgggcggcg cgcaggcggc
     2581 gggcgtgctg gcgaccgtgc ggcgcgagaa catcgaggcg gagcgtggaa cctggagcgc
     2641 ggaagaggaa gcggcgttca aggcgccaat tctgcagaat tttgagcgcg agggtcaccc
     2701 ctactacgcc accgcgcgcc tgtgggatga cggcatcatc acccccgggg agacgcgacg
     2761 ggtagtggcg ctggcactct cggccagcct caacgcgcca atcccgccgc cgcgctttgg
     2821 cgtcttccgt atgtagagct cgcgttcgcg ccgcgccagc caaacccttg cacctcgaat
     2881 ggaccctcaa gacatgaccg agcccctgct cttcacgccg ctcgcgctga agagcgtaac
     2941 cttgaagaac cgcgtggtgg tcgcaccgat gcatcaatat gcggccgtgc gtggacacgc
     3001 cacggactgg catctcgtca atgccggacg ctacgcgcag ggtggggccg ggctggtgat
     3061 catggaatcc accaaaatcg cgcgcaacgg ttgtggcacg gtcggcgacg ttggcctgtg
     3121 ggctgacgcc ttcatcccgc cgctcgcgcg ctgtgcccaa tttatcaagg cgcacggcgc
     3181 cgtcgctggc ctgcagctcg gccactccgg tcgcaaggcg cgcctgacgc ggccctggga
     3241 aggcggcaag ccgttgacgg gcacggaggc ggaagtcttc gattgggagg cctgggatct
     3301 ggtggcgccc agcgccgtgc cccacaccga gcgttcgccg atcccgcgcg ccttgtcgct
     3361 gcaggaggtg cgtgagctgg tggcgaagtg gggcgcggcg gcggcacgcg ccgtcaaggc
     3421 gggtttcgac gtcttggaaa tccacgccgc gcacggctat ctcgtgcacc aatttctgtc
     3481 cccgcgcgcg aacctgcgcg gcgacgccta cggcggctcg gagctcaatc gcatgcgctt
     3541 tgccctggag gtggccgagg ctgtgcgggc tgcctggccg aacgacaagc cgctctttat
     3601 gcggctttcc tgcgaggacg atgcgggctg ggacctcgag caaagcatca ggttggcgcg
     3661 gctgctcaaa gcttcgggta tcgatgtcat cgattgctcg tccgggggta ccttggcgca
     3721 ctccccgatg gagggggtgc gggcgaagaa gtatggctat caggtgccct acgcggaaag
     3781 aattcgccgc gaggcgggca tcatgacgat ggccgtgggc catatcgtgc atgccgatca
     3841 ggccgaggcc attttgcgcg agaaccgcgc cgacctcgtc gcgctggcgc gcgagatcat
     3901 gcacaatccg agctggccga tggacgcggc gcagaagctg ggggtggacc cccagttcag
     3961 cttggtgcct ccagcctacg cctattggct ggcaaaacgc gccgcttccg gtttcgaggg
     4021 cacgccgtcg acctggagca agggtctgcc gcctcgcggc gagccagcgg acgcctaggc
     4081 gagcgcgcgc cgcgtgcggg ggcctgagcc aaggcggcgg ccgagcctgg gagcctcagc
     4141 ttggggcgcg cgcacgcctt ttcgagcgcg cccctgaagt gctatcccct tggggtcgtt
     4201 cggcgacagc gcaatcgggc tcacacagcg agggacgcgg tcatgcctct ttacggcagc
     4261 gaagcactga aacgcatacg cgatggcaag ctggcgctgg gacttggcgt caaccatttg
     4321 cgcggttcgg ccgttccctt gctcgccaag gccgccggat atgattggct ttttatcgat
     4381 gccgagcacg gcgccatttc gacgccggaa atcgcgcaga ttgccatggc cgcgctgccc
     4441 atgggcgtgg tgccgatcgt gcgcatttgt agcggcgccc tggatgaggg gacgcgggcg
     4501 ctcgacaatg gggcgcttgg cattgtggtg ccgcacgtgg atacccccga gcaggcgcgg
     4561 gagctcgtcg aagctttccg cttcagcccg atgggacacc gcagcaccgg cggctcgaat
     4621 gcctgcttcg gctatcggcc gccgtcggcg gcggaggccc agaagacctt gaatgcgcaa
     4681 atcctggtgg taccgatgat cgagacgccg cgagcggtgg agaatgcgag cgcgatcgcc
     4741 gccatcgaag gcatcgatgt gctgctgatc ggcagcaacg acctctcgct cgagatggga
     4801 attccggggc aggtcggaca cgagcgcgtg cgggcggcct atgagacggt cgctgcggcc
     4861 tgtcgccagc acggcaaggt gctcggcatg ggcggcgttt acgatcagga ggtggcgagc
     4921 cgctatatcg ggcttggcgc gcgcatgatc cttggcgcca gcgaccacag cctgctgctg
     4981 gaggcggcca cgcggcgcgc ggagttcctg cgctccttgg cgccgcaatc cgctgcttga
     5041 ggcgaggctt gccggcaggc gcgtccgtgc ggcctcgcta gcccgccgcg cgcacacgcg
     5101 ctcccagccg ctcgatcagg gccatagcgg cggcgggggc gcggaccttg ccttcgtgga
     5161 tcacgtaaag aaagacgtcg cgggcggcct gtttggggac cttggccgtg agcgacggca
     5221 agtcggctgg ctgtccaccc gcggcccaag tcgcggcgcg cttggcccag gcatcgagct
     5281 cgcgcggtgg gtagcccgtg gcaatcgcat catcgccttt ttgcagacgt gcgtagacga
     5341 agtcgccgac cgggtcggcg atggccggat aggtgtcgtg ctcggcaaac acgatcgggg
     5401 ttttgtactt gcgcacgagc gcgatgaatt ccggggtcgc gaacgaggga tggcgcacct
     5461 ccacggcatg gcgcagcttg cgcccggcga gttcgctcgg cagcagggcg agaaaggcgg
     5521 caaagtcggt ggcgtcgaac ttcttgcttg gcgcgaactg ccacaggatc ggccccaagc
     5581 ggtcgcccag ttcgccgagg cccgacttga agaaaaacgc cagcgcctcg ccggcttcgg
     5641 ccagcacgcg cttctgcacg acatagcgcg ggcccttgac cgcgaacacg aagccgtcgg
     5701 gcacttcctg gcgccatttg gcgaaggtgg cgggcgtttg cgtgcggtag aacgtgccgt
     5761 tgatctcgat ggtggtgagc ttggagctgg cataggcaag ctccttggcg tgcgccaggc
     5821 ccttgggata aaacaccccg cgccagggct cgaaagtcca gccgccgatg ccaaccctga
     5881 tgctgcccag cttgggcatc gatgttcctc cccgtgatgc ggcttggcca tgctcggcaa
     5941 aactcgccgc tggcgcaaga ggggacgctg ccgctaaact gagacccacg ccatgccgtc
     6001 gagatcgcgc ccgcagtccg cgaaggtttc aagcccgtga gcatcaccag gggccggtgt
     6061 cagtgccgcg ccatcgttta tgagttcgag ggaccgccga agtgggtgat gcactgtcat
     6121 tgtgccgact gccgtcgcgc cgtgtcctcg gcgctcgcca cttatatcgg cgttcgtcgc
     6181 gatcagttcc gctatcttgc aggcgagccc gccgcctatg cctcctctga gggcgtcaaa
     6241 cgctattttt gtcgcgcgtg cggcacgccc atcgcctaca ccggcgcgaa ttggccggac
     6301 gaagtgcacc tgttgcacgg gagcctcgac gatccatcgc aatggccgcc gaccgggcat
     6361 gcctacgtgg gcgagcagat ccattggttc gacgttgccg atcagctgcc gcgctatctg
     6421 acgaccgcgg gcaagggcgc caagcccgtg cgcaacggac cgcgcacctg aaaggcaaag
     6481 gggtagcgtc ggcccgcgcg ccacgcctgt tggcctgccg cggggatccg ccatctctgc
     6541 accacaccgc catcgccttt cccctctgat ggagagcgcg gcggctgctc ggcgccgtcg
     6601 cggcatcgcc gccggccacc atggtgcggc cgatccgagg gttggccgcg ctggggctgt
     6661 gagcgatggc gggcgccagc tcgatgctgt tcgctgccga ggcaagccat ggggccacca
     6721 atcccgggtg ccagatcgag ctggtgttga tcgggctcgg catcccaagg cgctgctggt
     6781 cggcaggctg ctgcgggatg ccctcgatca aacgccggcc tcaatgccgc tgtcgacctc
     6841 gcccgcctcg ttgccgtgct atcgttgccg atctggtttg caacggccgc ttgtggtcgc
     6901 ctcatcgcgt agttttgaag gccgcggcgc cgtcggggtg cacgcaaagg ggatgtttcg
     6961 tggtttagtg ttcgccgagg ggtcacggca atggctggat cagcaggtcc gcaaaggcgc
     7021 tgcgccgcac cgtgaccgcg catgagccag ggcgcctcgc cggaacaaac gagacgaaac
     7081 gcgaagaaac gagaagaatc gagaagagac gagaacaaac gagaccaaac gagaccaaac
     7141 gagaccaaac gagaagagac gagaggatca ccaccgatgc ggcttttcac cgtgcttggc
     7201 ctgagcctgc tggcgctgtt tgcgcttgcc gcggaggccg gcgccaccga caagaagaag
     7261 aaagcgcaac gacacgccag tgcaccggca gccgctgcca cgccgcggcc ggatcgctac
     7321 cgcgaattgc tcgccgacaa aatgcccgtg gggtccagcg agtggtggga gcagatgcgg
     7381 cgcgaggggc gtcttggcgg ggaaacgccc tgaccgcatg ggccgggcgg gcctggcggc
     7441 acaaaaattg acgttgagag gggcgccgtt gggtgctggc gtcgctgggc cgacgtgcta
     7501 tagcgcggtc atgccagagc ctgtcaaacc caatgccgcc gtcgccgtcg gcagcgtgtc
     7561 ctttgccaac gacgcgccgc tcgccctgtt tgccggcccc tgccagatgg agagccggca
     7621 gcacgcgctg gagatggcct ccgccttgaa ggagatctgc gggcgcctgg gcgtcggtct
     7681 cgtgtataag acgtcctttg acaaagccaa ccgcacgagc ctcgcctcca agcgtggcgt
     7741 cggcctcgac ggcgcgctgg cggtctttgc cgaaatccgc gccagcctgg gtttgccggt
     7801 cgtcaccgac gtgcatgagg cgagccaatg tgcgcgcgtc gccgaggtgg tcgacgtgct
     7861 gcagatcccg gccttcctgt gccgccagac cgacctgttg gtggccgcgg ccgagaccgg
     7921 acggccggtc aaggtcaaga aggggcagtt tcttgcgccc tgggacatgc gccatgtggt
     7981 ggccaaagtt acgggcagcg gcaatgccaa cgtgctggtc accgaacggg gtgcctcgtt
     8041 tggctacaac acgctggtgg tcgacatgcg cgccctgccg atcctgcggg agatcggctg
     8101 cccggtgatc ttcgatgcga cccatgctgt acagcaaccg ggtgggcagg gtgcgcagtc
     8161 cggcggtgag cgccgctttg tggcacccct agcgcgcgcc gccgtggccg tcggcattgc
     8221 cggcctcttc atcgaaacgc atgaggaccc cgatcgggcg ccctccgatg ggcccaacat
     8281 ggtgccgctg aaagacttcg aaggcctgct cggcgaactg atggcgatcg acaggctggt
     8341 gaagcgccgt caggcctaga gcccgtgcca gggctggaac aggcgggcca gcaagccgtg
     8401 aaagcgcgtc ggcacctcgt tggcgacggc acacacgcag ccgcgcttcg gatcggcgat
     8461 gggtgtgtgc tcgatttcct gatcgaggtc ggcaacgtcg ccctgcccgt agctcccggt
     8521 ggcatcgctc agggcgccgc gcaggatcaa ggtcagctcc gcgccgccat ggccgtgctc
     8581 gggaaccgag gcgctcggcg ccgccttgat cagctgcagg gtgccggtgc ccgtcagcgg
     8641 caggggatgg tgccatagac cgaggccgat gcggcgccag ctgacggtag cgaaattctg
     8701 agcctcgagc cgcagaacgg gattggcgct gttgccgccg accggcgcgc tgctcgccgg
     8761 cgcgcccggc gccggcaggg cgggcacccc cgtcgtcagg ggcagcggcg ccacgtcgtc
     8821 gagcaaggct cccccgatgc gctcgaacca gacgagctcg tcgcggcagc ggcggcaaag
     8881 cgcgatatgc gcgcccgcga cggcggccaa agcgccgggc aaggttcccg cggcgaagct
     8941 catcagggtc tcgctcccga gatggtgcgt gatcgtcatt tcagatcctc gaccgcagtc
     9001 cgcatgcgtt gataggccat gcgcacgcgc gatttgacgg tgcccagcgg cacgcccagg
     9061 cgggcggcga tttcaccgtg cgacaagccc tgcagatagg cgagctcgag cacgcgctgc
     9121 tgctcggcgg gaagttcggc cagcgccgcg cggaccctga cctgcacctc gttttccgcg
     9181 aggagggtat cgggagaggg gtcgggggag gctgcctccg cgtgcgccgc cggcagctcc
     9241 tgccatggta cctcccggcg caggcggtcg atccacaggt tgcgggcaat gctaaagatc
     9301 caggtgctgg cgctgccctt ggcgctggaa aacagccccg cccgccgcca aacggccaac
     9361 agcgtttcct gggcgaggtc ttcggccgtc gccgcgtcgg ccccgcggcg cacgaggtag
     9421 gccttcagcc tcgggccata gctttgatag agctcatgaa aagcagcagc atcgctctct
     9481 tgcgcaatgc gggccaagag ctctgacatg gcgttcaaat tcatcccggc cgagggcccg
     9541 ggcacggccc tgaacgacac ccaattgccg agcatagcca accctcgacc gttgcccaag
     9601 gagtacggtc ggcgcaccgg cttggatcaa ctcggcgcct gggcgccctt ttccttgccc
     9661 agagtgggga ctatcgtgcg tcgaggcggt cttttccaag gggtctcagg gcctcccggc
     9721 gctgggcgaa ggcatggcag cggccaggcg gatcatctgt gttgggcatg cggcgctcga
     9781 tcgcatctat cgcatcgagg cctttccgcc ggagcctagc aaagtgcgcg ccctggagca
     9841 catcgaggcc ggcggcggca tggcggcgaa tgccgccgtg gcgatcgcgc ggctgggcgg
     9901 caaggccgag ctctggagcc gaaccggcga tgatgccgcg ggcgccacgg tgcgcgccgg
     9961 cctcaaagcc gagcgcgtcg acgtgcgtta tctgcaggcc tttgagggcg cccgcacctc
    10021 gacctcggcc atcatcgtcg atcggcacgg cgagcgcctg atcgtcgggc agcgcgacgc
    10081 cggcatgcca tcgggtacca gttggctgcc gctcgagcgc gtgcgcgagg ccgatgcggt
    10141 gttgggcgat ctgcgctggc tcgaagcctt acgctcggtg ttcgcgcggg cccggcagga
    10201 gggggtcccc accgtgctcg atgcggacct cggcgcgcgc gaagcgctcg agagcatttt
    10261 gcggctgacc gattatgccg tcttctccgc gccggcgctg cgggagctgg tgccggcggg
    10321 ctcggacgcg gagcggctcg agagcgtctt gggcttcggg gcgcgctacg ccggggtcac
    10381 gctgggagct gacggctatc tctggcgtga tcaggagggg acccgtcggc tgccagcctt
    10441 tggcgttgcc gtggccgaca ccaccggggc gggggatgcc ttccacggcg cgctcgcctt
    10501 gctgttggcg gagggccgtc ccatgcacga gtgcgcgcgc tcggcaagcg cggtcgctgc
    10561 catgaagtgc acacggctcg gctcgcgctc gggtttgccc acccgtgcgg aactgacggc
    10621 ctttctggcg acgcacgcgg cgtgatcgcg ccggccgcgc ggacttatcc acagctttca
    10681 aaatgcctcc gcaagagttg atttgatttc tgatgcgctg cccagtgggg cggctagaat
    10741 cgccgctagg gcatttcagt agcggaggaa gacgttaatg gctgacaaga acagcagtgg
    10801 gcgagtggaa aatggcttca tgccgatgcc tctgcctctc ggcttcgaag cctttatgga
    10861 gatgcagcgg cccgcgctga cggcaatggc tcagatgaac ggcaaggttt atgacaatct
    10921 ggccgcgatc aaccgcaatt gggtgacctt cgtcaatcgc cgcctgaagg aagagctggc
    10981 gatgccgcag aacctggcag gctgcaagac ggtgcaggat atgtacggcg tctacagcga
    11041 attctttcag accgccgtcg ccgactatca gtccgagttc gagcaaatga caaagctcgg
    11101 caagtcgctt gccgaggaca ccgcccagtt cgtgcaagcg cgcatcgatg atgtcgcccg
    11161 tgaggggcgc aacggccgct gagacggcaa cgctggcgag gcgtgaggtt cgggcgggcg
    11221 cgcgggcgag gctgcccgtg cgccacggca agggcatcag tcctcgagct tgtgccccag
    11281 gcgtcccgcc aggtcctgga tgaactgcca ggccacccgg cccgagcgcg cgccgcgcgt
    11341 catcgaccac tccaaggctt cgcgcttgag ttcatcggcg tcgagcttta gccggtgatg
    11401 cgccgcgtag ctgcgcacca tctccaggta ctcgtcctgg gtggcgttgt gaaagccgag
    11461 ccacaagccg aagcgatcgg agagcgagac cttttcctcc acggcttcgg aggggttgat
    11521 ggccgtcgac ctttcattct ccatcatgtc gcgcggcatc agatggcggc ggttcgaggt
    11581 ggcgtaaaac accacgttcg gcgggcggcc ttcgatgccc ccctccaaca cggccttcaa
    11641 ggacttgtag gaggtatcgt ccttgtcgaa ggaaaggtca tcgcaaaaca cgataaagcg
    11701 gtgcgcggcg gtgcgcaggt gcgctaggca ttgcggcaag gcggcaatgt cctcgcgatg
    11761 gatctcgatc agcttgagat cgggcttgcc gctggcgcgg ccaagaccca aggtgcgccg
    11821 cacctccgcc tgcgccgcct tgacgaggga ggatttgccc atgccgcgcg cgccccacaa
    11881 caaggcgttg ttggcgggca ggccggcggc aaaacggcgg gtattgtcga gcagctgttc
    11941 ggccgcgcga tcaatgccct tgagcagcgc gagtggttgc cggttgacgc gcgccaccgg
    12001 caggaaggct tccggg
//
LOCUS       DASTMT010000302         3336 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_826029, whole genome shotgun sequence.
ACCESSION   DASTMT010000302 DASTMT010000000
VERSION     DASTMT010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3336)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3336)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3336
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_826029"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..781
                     /locus_tag="VFR00_06995"
     CDS             <1..781
                     /locus_tag="VFR00_06995"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEU0159040.1"
                     /translation="ELFATQLFERHHKRLSLRPDGERLIVHAHRLIAQNDEVWGAMSA
                     PAYEGEVTLGVPTDIVGVFIPPILKRFDKAWPRVRVSLKCSTTPQLLEALRKADIDLT
                     LTTEQACRPHGETLLEDELVWAGAVNGVAHRREPLPISLGRETCEFRPSVLQALRDGK
                     RDWRPVCESGMDALKASLDADVAVGPFLRRTIPDYLRMVDDPRLPRLPRFLINMYLPP
                     ARQSEIAVELARHIRAEFDLRFRRTLAPERRKPMPVAALAS"
     gene            799..1683
                     /locus_tag="VFR00_07000"
     CDS             799..1683
                     /locus_tag="VFR00_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU0159041.1"
                     /translation="MASFATLMQPWRRRHWRADPAALAAYAGLRPFTVVTGGSDGIGL
                     ALARRFAGAGNDLLLIARRPEPLQAAADRIRAESRVEVATAALDVTAAGAIAALEAAL
                     AARGGYADVLVNCAGIGFAGRFGEQPPEALAQLLDLNVRALTMLSRHFLSGMRVRGRG
                     GILNLASLGGYVPGPNQAAYYASKAYVLSLSEALAAETAGEGVRICALAPGPVDTRFH
                     ARMGAQGALYRTLLPPASAAAVARAGYIGFALGWRVTIPGLINPFLQLALRVMPHRLV
                     IPIVGWLLQPRGQTLTNA"
     gene            1676..2497
                     /locus_tag="VFR00_07005"
     CDS             1676..2497
                     /locus_tag="VFR00_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008551472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HEU0159042.1"
                     /translation="MPDAISPARSQDRAIGQIPPANPKVAAKAPVCIILHQENSNPGH
                     VGQWFVRNGYSLDIRKPRFGDPLPPTLEHHCGCVIFGGPMSANDKDAFVRTEIDWIGV
                     PLRERKPFLGICLGAQMLTVNLGAKVGFHPEEFVEIGYYPLLASEPGGDALGQLPDHV
                     YEWHKEGFELASGARLIATSPGAFPNQAFIYGPAAVGVQFHPEITYAQVHRWTGHNPQ
                     RLAMKGARARQQHIEGHVTHGPKVQAWLDRFLSRWICGRLIEPAAAVTIGPARAA"
     gene            complement(2536..>3336)
                     /locus_tag="VFR00_07010"
     CDS             complement(2536..>3336)
                     /locus_tag="VFR00_07010"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HEU0159043.1"
                     /translation="LGAAVTLQQIERHPGVAAHYAAVAEAAASVAGPTQRNMGTLGGN
                     LCLDTRCIFYNQSEWWRAANNHCLKTTGEICHVAPKSRGVCFATFSGDLAPALAVLDA
                     KVDLLAADGTARTLPLEQMYVGYARHDDAAAGDGKHYLALRPGEMVTGVRAHREPGLR
                     SGYDKLRVRRSIEYPLAGVAVALRREGDCLTHLRVAVTGTNPRPVLLKGTERLCGGPL
                     DADLYQGLEHLVRDQVMSMKTTFTPGHYRRRVAAILAKRLVSRLFNAA"
BASE COUNT          529 a         1124 c         1107 g          576 t
ORIGIN      
        1 agagctgttc gccacgcaac tgtttgagcg ccatcataag cgtttgagcc tgcggcccga
       61 cggcgagcgg ctgatcgtgc acgcacatcg tctgatcgcc caaaacgacg aggtctgggg
      121 tgccatgagc gcacccgcct acgaaggcga ggtgacgctc ggcgtgccga ccgacattgt
      181 cggcgtcttc attcccccaa tcctcaagcg tttcgacaag gcgtggccgc gcgtgcgggt
      241 ttccctgaaa tgcagcacca cgccgcagct gctcgaggcc ctgcgcaagg ccgacatcga
      301 cctgaccttg accaccgaac aggcctgccg gccgcacggc gaaaccttgc tagaggacga
      361 gttggtgtgg gcaggtgccg tcaacggcgt cgcgcatcgc cgcgagcctc tgccgatctc
      421 gctgggacgg gaaacttgtg agtttcgtcc ctcggtgctg caggccttgc gcgacggcaa
      481 acgcgactgg cggcccgtgt gcgagagcgg catggacgcg ctcaaggcct ccctcgacgc
      541 cgacgtcgcc gtcggaccgt tcctgcgccg cacgatcccc gactacctta gaatggttga
      601 cgatccccgg ttgccgcggt tgcccagatt tctgatcaac atgtacctgc cgccggcgcg
      661 tcagagcgaa attgccgtcg agctcgcccg ccatattcgc gccgagttcg acttgcgctt
      721 tcgccgcacg ctggcgccgg agcggcgcaa gccgatgccg gttgcggcgc tcgcgagttg
      781 aggcggcggg cagtcggcat ggcttcgttc gccactctga tgcaaccctg gaggcggcgg
      841 cactggcggg ccgacccggc ggcgctcgcc gcctatgctg ggctacggcc cttcacggtc
      901 gtcaccggcg gatcggacgg tattggcctg gctctggcgc gccgctttgc cggtgcaggc
      961 aatgacctgc tattgatcgc gcgtcggccg gagccgctgc aggcggcagc cgaccgcatc
     1021 cgtgccgaaa gccgggtcga ggtcgccacc gcggcactcg acgtcaccgc agccggcgcc
     1081 attgccgctc tcgaagcggc gttggcggcc cgcggcggct atgccgacgt tctggtcaac
     1141 tgcgccggca tcggcttcgc cgggcggttc ggcgagcagc cgccggaggc gctggcgcag
     1201 ttgctcgatt tgaacgtgcg ggcattgacg atgctgagcc ggcatttcct cagcggcatg
     1261 cgcgtgcgtg gacgcggcgg cattctcaat ttggcctccc tcggcggcta cgtcccgggt
     1321 cccaatcagg ccgcctacta cgccagcaag gcctacgttc tgtccctgtc ggaggccttg
     1381 gccgccgaaa cggcgggtga gggggttcgc atctgcgcct tggcaccggg gccggtcgac
     1441 acccgcttcc acgcccgcat gggcgcgcaa ggcgcgctct accgcaccct gctgccgccc
     1501 gcgtccgcgg cggcggtcgc acgcgccggc tatattggct ttgccctcgg ttggcgggtg
     1561 acaatccccg ggctgatcaa cccttttttg cagctggcgc tacgtgtcat gccgcataga
     1621 ctagtgattc ccattgtcgg atggttgctg caaccacgag gccagaccct gaccaatgcc
     1681 tgacgccatc tcccccgcgc gctcgcaaga ccgcgcgatc ggccagatcc cgccagcgaa
     1741 cccgaaggtt gcggccaagg cccccgtctg catcattctg caccaggaga attccaatcc
     1801 cggccatgtc ggccaatggt ttgtccgcaa cggctacagc ctcgatattc gcaagccccg
     1861 cttcggcgat ccgctgccgc cgacgctgga gcatcactgc ggttgcgtca tattcggcgg
     1921 accaatgagt gccaacgaca aggacgcgtt cgtgcgcacc gaaatcgact ggattggtgt
     1981 gccgttgcga gaacgcaagc cgtttctggg catctgcctc ggtgcgcaga tgttgaccgt
     2041 caatcttggc gccaaggtcg gcttccatcc cgaggagttc gtcgaaatcg gctactaccc
     2101 tctgcttgcc agcgagccgg gcggcgacgc gctcgggcaa ttgcccgatc atgtctacga
     2161 gtggcataag gaaggcttcg agctggcgag cggggcgcgc ttgatcgcca cctcgccagg
     2221 ggctttcccc aaccaggcct tcatctatgg cccggcggcc gtgggggtac agtttcatcc
     2281 cgagatcact tacgcccagg tgcaccggtg gaccggccac aacccccagc gcctcgccat
     2341 gaagggggcc cgggcgcgac agcagcacat cgaaggccac gtcacgcacg gccccaaggt
     2401 gcaagcctgg ctcgaccgct tcctcagccg ctggatctgc ggccggctga tcgaaccggc
     2461 agcggccgtg accataggtc ctgcaagggc ggcttgacgc caagcgcacg ccaagcaccg
     2521 ggcacgtcgc cccgctcaag ctgcattaaa tagccggctg acgagccgtt ttgccaagat
     2581 ggcggcgacg cgccggcgat agtgcccggg cgtaaaggtc gtcttcatgg acatgacctg
     2641 gtcgcgcacg aggtgttcga ggccttggta gaggtcggca tcgagcggac cgccgcagag
     2701 acgctcggtg cctttgagta gcaccggtct gggattggtg cccgtgactg ccacgcgcaa
     2761 gtgcgtgagg caatcgccct cccgacgcaa ggccacggca acgccggcga gcggatattc
     2821 gatcgagcgg cgcacgcgaa gcttgtcgta gcctgagcgc agaccgggct ctcggtgcgc
     2881 gcgcacgccg gtgaccatct cgcccgggcg tagcgccagg tagtgcttgc cgtcaccggc
     2941 agcagcatcg tcatggcgcg cgtagccgac gtacatctgc tcgagcggta gggtccgggc
     3001 cgtgccatcc gcggccagga ggtcgacctt ggcatcgagc acggccaggg cgggggccag
     3061 gtccccgctg aaggtggcga agcacacgcc gcggcttttg ggtgcgacgt ggcaaatctc
     3121 gccggtcgtc ttgagacagt ggttgttggc cgcgcgccac cattcgcttt gattgtagaa
     3181 gatgcagcgg gtatcgaggc aaaggttgcc gccaagcgtg cccatattgc gttgggttgg
     3241 tccagccacc gaggcggcgg cctcagccac ggcggcatag tgcgccgcca ccccgggatg
     3301 gcgctcgatt tgctgcagcg tcacggcggc gccgag
//
LOCUS       DASTMT010000303         2238 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_2462222, whole genome shotgun sequence.
ACCESSION   DASTMT010000303 DASTMT010000000
VERSION     DASTMT010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2238
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2462222"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..339
                     /locus_tag="VFR00_07015"
     CDS             <1..339
                     /locus_tag="VFR00_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004107869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HEU0159044.1"
                     /translation="VRNFLARSNMKAMRLGDRGFFYHSNVGKEIVGIVEVCALAHQDA
                     SDATGVWTCVDVRAIADMPKPVSLVAVKANRKLANMVLVRSSRLSVQPVTPEEWAEVC
                     RMGDLDPKSI"
     gene            830..1036
                     /locus_tag="VFR00_07020"
     CDS             830..1036
                     /locus_tag="VFR00_07020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0159045.1"
                     /translation="MQALEQIEDFWSDTYRGRNIAILNRGGSWLVYLDHVMQHRMLFD
                     TAESAVRWLQRQIDQNRAPAYTKG"
     gene            complement(1147..2208)
                     /locus_tag="VFR00_07025"
     CDS             complement(1147..2208)
                     /locus_tag="VFR00_07025"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEU0159046.1"
                     /translation="MGKKIVIVGTGAVGGYAGAHMVQAGEDVTLIDPWPAHVDHMRQH
                     GLRVTHARDVAEFSVTVRALHLTEVQQLSKEKPVDIAFICTKSYDTAWAAMLIKQYVA
                     ADGYLVSLQNCMNEETIAGVVGWGKTLGCIASSITVNLPDPGHIHRGAGKRGAGHTVF
                     RAGEVHGRITRRAEEVCRLVGYADSAKATDNLWGERWSKLVANVMGNGLSACTGLTGA
                     QMLDIDAIRRFSTRLGSEAIRAGQALGYQLEDILHLPPETIARAGEGDEAAMRTCDEQ
                     RRKDGKATSAEQRPSMGQDMQKGRRTEIEFLNGYVAREAMRLGLPCRANEALTDIVKR
                     VERGELAPDPRHITELRLN"
BASE COUNT          368 a          741 c          714 g          415 t
ORIGIN      
        1 gtgcgcaatt tcctcgctcg cagcaacatg aaggcgatgc gcctgggcga ccgcggtttt
       61 ttttatcact ccaacgtcgg caaggagatc gttggcatcg tcgaagtctg tgcgcttgca
      121 catcaggatg ccagtgacgc aacgggcgta tggacgtgcg tcgacgtccg cgccatcgcc
      181 gacatgccca aaccggtgag cctggttgcc gtcaaggcga accgcaagct cgcgaacatg
      241 gtcctggtgc gctcctcgcg cctttccgtg caaccggtca cgccggagga atgggccgaa
      301 gtctgtcgca tgggcgacct cgatcccaag tcgatctgac acaaaccgtt ccggtccgaa
      361 ccattctgcc cgctccggct catactattt tggacgttag atgagttgag ccgggtcgac
      421 ctgcacgcgt gcataggcgt ccggatggct ggcgagcttg cctcaagcgc cggcgcgggg
      481 ccgtgcaact gccgcgaaag gcagcaaccg ccagggtctg ttgccgacga agtgatcagg
      541 cccgacagcc ggcaaggccg cggcggcgtc acggcggacc gtcggcagcg caaactgcgg
      601 ctttttctcg gccacaccgt cgcacctttc tgcgcttgca cgatagttgc ttgatcgtgg
      661 ccaggccggt gcggccccgg gggccagcgc gcctcaccgg actaaagtgc tggcgcgggc
      721 gcgtgagagc tcacggcgac ggagcgggaa cacccctacg ttttgagggt taagcccgca
      781 ataaggcccc cgctaactgg gccaggagcg atgattgtga ggcaagatca tgcaggcact
      841 tgagcagatc gaggattttt ggtcggacac ctaccgcggg cggaatattg ccattcttaa
      901 ccgcggcggc agctggctcg tctacttgga ccacgtgatg cagcatcgca tgctgttcga
      961 cacggccgag tcggccgtgc gctggctgca gcggcaaatc gaccagaacc gcgcgcccgc
     1021 ttacactaaa ggctaacgcc cacgcggcga tgtcagatcg cgcccgcccc cgacggcgtt
     1081 gccctcctcc ggcaacagac gcgcgccccg aggcgcattg gcgcgccagc aggtttcggc
     1141 ccgctgtcag ttcagccgca gttcggtgat gtggcgcggg tcgggcgcca attcgccccg
     1201 ctcgacgcgc ttgacgatgt cggtgagggc ttcgttggcc cggcagggca ggccgagccg
     1261 catcgcctcg cgtgccacgt aaccgttgag gaactcgatc tcggtgcgcc gtcccttttg
     1321 catgtcctga cccatcgacg gccgctgctc ggccgaggtg gccttgccgt ccttgcgccg
     1381 ctgctcgtcg cacgtgcgca tggcggcctc atcgccctcc ccggcgcggg cgatagtctc
     1441 tggcggcaaa tgcaaaatgt cctcgagctg atagccgagc gcctgtcccg cgcggatggc
     1501 ctcgctgcca aggcgcgtcg agaagcgacg gatggcgtca atgtccaaca tttgtgcgcc
     1561 cgtgaggccc gtgcatgccg acaggccgtt gcccatgacg ttggcgacga gtttcgacca
     1621 gcgttccccc cacaggttgt cggtggcttt ggcgctgtcg gcgtagccga ccagccggca
     1681 cacctcctcg gcgcggcggg taatgcgccc gtgcacctcg ccggccctga acacggtgtg
     1741 gcccgcaccg cgtttacccg caccgcgatg aatgtgtccc gggtcgggca aattgaccgt
     1801 gatcgagctg gcgatgcacc ccagcgtctt gccccagccg acgaccccgg cgatcgtttc
     1861 ctcgttcata cagttctgca gcgagaccag gtaaccgtcc gcggccacgt attgcttaat
     1921 gagcatggcc gcccaggccg tgtcgtagga tttcgtgcag atgaaggcga tgtcgaccgg
     1981 cttttccttg ctcagctgtt gcacctcggt gagatgcaag gcgcgcaccg tcacgctgaa
     2041 ttccgccacg tcgcgggcgt gggttacgcg caggccatgt tggcgcatgt gatcgacgtg
     2101 ggcgggccat ggatcgatga gggttacgtc ttcacccgcc tgcaccatat gcgcgccggc
     2161 atagccgcca accgcgccgg taccaacgat cacgatcttc ttgcccatgg tgcgtctccc
     2221 aacctcatcg ccggtcag
//
LOCUS       DASTMT010000304         4829 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_500359, whole genome shotgun sequence.
ACCESSION   DASTMT010000304 DASTMT010000000
VERSION     DASTMT010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4829)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4829)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4829
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_500359"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            128..976
                     /locus_tag="VFR00_07030"
     CDS             128..976
                     /locus_tag="VFR00_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018860257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEU0159047.1"
                     /translation="MVTAAAAIGQNQQPPGINLKELKARQHGAWSSGDYAVVGTTLQI
                     VGEQLCESLDLRAGQHVLDVAAGNGNVSLAAARRWCDVVSTDYVPSLLERGRMRATAE
                     ALPVRFQEADAEALPFEAGTFDVVVSTFGVMFTPNHEQAASELLRVLKPGGKIGLANW
                     TPQGFIGQVFKTLGKYLPPPAGAKSPALWGTPQHLGQLFGAGAASIKTEGRHFVFRYR
                     SAEHFVDIFKAYYGPMLKAFAALDTAGQQSLRRDLIALIGSMNRGSDASMAVPSEYLE
                     IIIAKR"
     gene            complement(1033..1959)
                     /locus_tag="VFR00_07035"
     CDS             complement(1033..1959)
                     /locus_tag="VFR00_07035"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0159048.1"
                     /translation="MAIVLQETLRAVFYAPFYAALSLHAFRQEGVEVSLITAPAPSAA
                     ARGLADGSADVAWGGPMRVLHTYDTDPACDLVCFCEVVTRDPFFLLGRTAKPDFRLSD
                     LAGLRLATVSEVPTPWMCLQEDLRRAGLDPAKLDRVDDRSMAQNCDALRRGEVDVVQL
                     FQPFVEELVEEHAGHIWSAAAERGPTSYTTLYAPRALLAARRRDCEGMVRALHRTLQW
                     VHAGDGSRLAAAIADYFPHLPQARLAKALARYQALGLWGRNPHLPRIGYERLKAGLLS
                     GGLIKTAAPFERVVDNSLADLATNQQLPPLTH"
     gene            complement(2023..2868)
                     /locus_tag="VFR00_07040"
     CDS             complement(2023..2868)
                     /locus_tag="VFR00_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012110922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HEU0159049.1"
                     /translation="MRNRQIEVRPIAGALGAEIHNVDVSQELDAATIGDIRQALLEHC
                     VIFFRNQRLDAERHKAFTRRFGSLFIHPNYRGMQADPELVVITREPGDKHIVGEDWHA
                     DTTMVAEPPMGAILYAVEAPPYGGDTLFANQYQAYESLSEGMQQLLSRLKAVHSDRTV
                     AGPRANRNAQRSTKVREDAEWRETVSTHPVVVTHPETGRKLLYVNRSYTQGFEGMTEE
                     ESRPLLEYLLEHGHRPEFTCRFRWEPGSIAFWDNRCCKHLAIHDAGPFRRVMRRTQIC
                     GSRPL"
     gene            3093..3368
                     /locus_tag="VFR00_07045"
     CDS             3093..3368
                     /locus_tag="VFR00_07045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0159050.1"
                     /translation="MQRGVMAVVTAVAIGLALVAGTSDLSLAQQSSVKTVAATAAPKA
                     GKGEKVCRVKLKYSGEVKTWVCKSEEPCCVWHEINYVKCGSTITGCL"
     gene            complement(3433..4113)
                     /locus_tag="VFR00_07050"
     CDS             complement(3433..4113)
                     /locus_tag="VFR00_07050"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein"
                     /protein_id="HEU0159051.1"
                     /translation="MKKLHSAVLAAVASVSVAQAAAAADLGGPRRGSLKDTPVADAPV
                     VYERPFSWSGLYIGANVGYGWSDVDWQDTIGPGSHSGTGWLAGAQIGYNVQTGQFVFG
                     AEADLSSAWIDGGNGCCGHEFNWLASVRARAGIAFNGNRTLLYATGGAAWADVDYTST
                     IGSGFSNTHFGWVAGGGIEHMLTQNLSARVEYLHYSFDEASGVAGGLATTVEPTLDTV
                     RFGLNLKF"
     gene            4530..>4829
                     /locus_tag="VFR00_07055"
     CDS             4530..>4829
                     /locus_tag="VFR00_07055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013947106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis isomerase"
                     /protein_id="HEU0159052.1"
                     /translation="MTALDYHVLDVFTTARFGGNPLAVVLDADGLDGQRMQTIAREFN
                     LSETVFVLKPQHGAHTAAVRIFTPAAEMPFAGHPTVGTACLLAQLRQPVTRDASDA"
BASE COUNT          837 a         1558 c         1627 g          807 t
ORIGIN      
        1 cttttccacg ccgccaccca cgttcgcctc cacgcggggt tgctagggat gctgatccga
       61 cgctcatggg ccgcaagtcg cgcccaggca agcaaaaacg gcaacagccg cacaacggag
      121 gacggtcatg gttacagcag ctgcagctat cggtcaaaac cagcagcccc caggcatcaa
      181 ccttaaggaa ttgaaggcgc gccagcacgg cgcctggtcg tccggcgact acgccgtggt
      241 cggcaccacc ttgcaaattg tgggtgaaca actgtgcgag agcctcgatc tgcgcgcggg
      301 ccagcacgtg ctcgacgtgg ccgccggcaa tggcaacgtc tcgctcgcgg cggcgcgccg
      361 ctggtgcgat gtggtgtcga ccgactacgt cccaagcctg ctcgagcgcg gccgcatgcg
      421 agccacggcc gaagcactcc cggtgaggtt ccaagaggcc gatgccgagg cgttgccctt
      481 cgaggccggt accttcgatg tggtcgtctc cactttcggc gttatgttca cgcccaacca
      541 cgagcaggcc gccagcgaac tcctgcgggt gttgaagccg ggcggtaaga tcgggttggc
      601 caactggacg ccgcagggct tcattggcca agtcttcaaa acgctcggca aatacctgcc
      661 gccgcccgcc ggcgccaagt ccccggcgct ttggggcacg ccgcagcatc tcggccaatt
      721 gttcggcgcg ggggcggctt cgatcaagac ggaagggcgc cacttcgtgt ttcgctaccg
      781 ctctgccgag cacttcgtcg acatcttcaa ggcctactat ggaccgatgc tgaaagcctt
      841 tgccgccctc gatacggcag gacagcaaag cctccggcgc gatctcatcg ccctgatcgg
      901 cagcatgaac cgcggcagcg acgccagcat ggcggtgccg agcgaatacc tggagatcat
      961 catcgccaag cgttgaggcc acggcaggcg cggcgcacca caccatttcg gtgcgtgcgc
     1021 cgccggcgcg cctcagtgcg taagcggcgg cagctgctgg ttcgtcgcga ggtcggccag
     1081 gctgttatcg acgacgcgct caaaaggcgc cgccgtctta atcagcccgc cggataggag
     1141 cccggccttg aggcgctcat aaccaatccg gggcaggtgc ggattgcgcc cccaaagtcc
     1201 gagcgcttga tagcgcgcga gcgccttggc gagccgcgct tgcggcaggt gcggaaaata
     1261 gtcggcgatg gcggcggcga gcctgctgcc atcgccggca tgtacccatt gcagcgtgcg
     1321 atggagtgcg cgcaccatgc cctcgcagtc cctgcgccgg gccgccagca gggcgcgggg
     1381 cgcgtacagc gtcgtatagg aggttggccc gcgctcagcg gccgccgacc agatgtggcc
     1441 cgcgtgctcc tcgacgagtt cctcgacgaa gggctggaag agctgcacca cgtcgacctc
     1501 gccgcgcctg agcgcgtcac agttctgcgc catcgaacga tcgtcgaccc ggtcgagctt
     1561 cgccggatcg agccccgccc ggcgcaaatc ctcctgcagg cacatccagg gcgtcggcac
     1621 ctcgctgacc gttgccaggc gcaggcctgc gagatcgctc aggcggaaat caggcttcgc
     1681 cgtgcggcca agcaaaaaaa acggatcgcg cgtgacgacc tcgcagaagc agacaaggtc
     1741 gcaggctggg tcggtgtcgt aggtatggag cacgcgcatg ggcccgcccc aggccacgtc
     1801 ggcgctgcca tcggccaatc cgcgtgccgc ggcggacggg gcgggagccg tgatgaggga
     1861 gacctcgacc ccttcttgcc ggaaggcgtg cagggaaagc gcggcataaa atggggcata
     1921 gaagacggca cgcagcgtct cctgcagcac gatggccata atcccggttc ctcctcgagg
     1981 cggcgggctg ctccctgccc ccgcggcgct tccgacccgc ctttagagtg ggcgcgagcc
     2041 gcagatttgg gtgcggcgca tcacgcgccg gaaaggtccg gcgtcatgga tggcgagatg
     2101 cttgcagcag cgattgtccc aaaaggcgat cgaccccggc tcccagcgga agcgacaggt
     2161 gaattcgggt cgatggccgt gctcgaggag atactcgagc agcgggcggc tttcctcctc
     2221 cgtcatgccc tcgaagcctt gcgtgtagga gcggttgacg tagagcagct tgcgccccgt
     2281 ctccggatgc gtgaccacga cgggatgggt gctgacggtc tcgcgccact ccgcgtcctc
     2341 gcgcaccttg gtggagcgct gcgcgttgcg gttggcgcgc ggcccggcga cggtgcggtc
     2401 cgagtgcacg gctttgagcc gtgacaatag ctgctgcatg ccctccgaca ggctctcgta
     2461 ggcctggtac tggttggcaa acagcgtgtc acctccatag ggaggcgctt ccacggcgta
     2521 gagaatggcc cccatcggcg gctcggcgac catggtggtg tccgcatgcc agtcctcgcc
     2581 gacaatgtgc ttatccccgg gctcgcgggt gatgacgacg agttcggggt cggcttgcat
     2641 gccgcggtag ttgggatgaa tgaagaggct gccgaaccgc cgcgtgaagg ccttgtggcg
     2701 ttccgcgtca agccgctggt tacgaaagaa aatcacgcag tgctcgagca gcgcctggcg
     2761 aatatcgccg atcgtggcgg cgtcgagctc ttggctgacg tcgacgttgt ggatttccgc
     2821 accgagcgcg ccggcgatcg ggcggacctc gatttgcctg ttgcgcatgg ggtttctctc
     2881 caggcgggcg gccgcgtcac agcgggcccg cgcaaaatta tgccgcaccg gcaggctatg
     2941 gcctgatctg gcgctcggca atagccggcg ccgattcgca ggcgtgcgaa atagccttgc
     3001 actgcggctt gcgcgcccta ggcgcagcgc ccaagcggcg gcagtatcag gcccagacga
     3061 ctctgtcctt tggcgacacc aggaggtttt ccatgcagcg aggcgtgatg gcggtggtaa
     3121 ccgctgtggc aataggcttg gcgctcgtgg ccgggacgag cgatctgtcg ctggcgcagc
     3181 aatcgagcgt caagacggtg gcggcgacgg ctgctcccaa ggccggcaag ggcgagaagg
     3241 tctgccgcgt caagctcaaa tactccggcg aggtcaagac ctgggtgtgc aagtcggaag
     3301 agccctgctg tgtgtggcac gagatcaact acgtgaagtg cggcagcacc atcacgggat
     3361 gcctctaggc ggccagcgaa aaggggcgcc gcaggcgccc ctcgcaagtg agcccgtttg
     3421 ggggctgcgc gttcagaatt tcaggttgag gccgaagcgc acggtatcca gggtcggctc
     3481 cacggtggtg gcgaggccgc ccgccactcc cgaggcctcg tcgaagctat agtgcagata
     3541 ttcgacgcgg gcgctgaggt tctgcgtcag catgtgttcg ataccgccgc ccgcgaccca
     3601 gccaaaatgt gtgttggaaa agcccgagcc gatggtcgag gtatagtcga cgtcggccca
     3661 ggccgcgccg cccgtggcgt agaggagggt acggttacca ttgaaggcga tgccggcgcg
     3721 cgcgcgcacc gaggccagcc aattgaactc gtggccgcag cagccattgc caccgtcgat
     3781 ccaggcgctc gacaaatccg cttcggcgcc gaacacgaac tggcccgttt gcacgttgta
     3841 gccgatctga gcaccggcca gccagccggt accgctgtgg cttcccggac cgatggtgtc
     3901 ctgccagtcg acgtcggacc agccgtagcc cacgttggcg ccgatataaa gaccactcca
     3961 ggaaaatggc cgctcgtaga cgaccggcgc gtcggcgacc ggcgtgtcct tgagactgcc
     4021 gcggcggggc ccgcccaggt cggcggcggc ggcggcctgg gcaacactga cactggcaac
     4081 ggcggccagc acggcagagt gcagtttttt caatggtatc cccttggtgt gtgctgcctg
     4141 caaacgacga tggagcagtt acggttccgc cggcagtgcg gcgatggtgt tgcgggcgat
     4201 caccgccgcc gctgtgtgaa agcgcgcgac tgcgcgccct tccggtccct gggttgcagc
     4261 gaagctcgca actgctcctt cccccgtcaa aaaccacgcc gtcagttcgc gtggtcgtgc
     4321 gcccgcgagt gtgcccccgc gtttgaccac agcgtggcgc gggggtggcg atcgcgtggc
     4381 agctgccggc tgggcgcaaa aattgatccg gctgcaacat cgcgaaacga agttgaaatc
     4441 tgccgcaggc gggggtgtgc gacggcggtc ttggaagtgc tggcgaaata cgccaatctg
     4501 cgggctctat aacaagagaa cccttgagca tgaccgccct cgattatcac gttcttgatg
     4561 ttttcacgac cgcacgcttc ggcggtaatc cgcttgccgt ggtgctcgac gcggacggcc
     4621 tcgatggcca acgcatgcaa acgatcgcgc gcgagttcaa cctatccgag acggtctttg
     4681 tgctgaaacc gcagcatggc gcccacacgg ctgccgttcg catctttacg ccggccgccg
     4741 aaatgccctt tgccggacac ccgaccgtgg gcaccgcctg cctgctggcg caattgcgcc
     4801 agcccgtgac gcgggacgcc agcgatgcg
//
LOCUS       DASTMT010000305        21663 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_1480952, whole genome shotgun sequence.
ACCESSION   DASTMT010000305 DASTMT010000000
VERSION     DASTMT010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 21663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21663
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1480952"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..191)
                     /locus_tag="VFR00_07060"
     CDS             complement(<1..191)
                     /locus_tag="VFR00_07060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002710946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEU0159053.1"
                     /translation="MTALSFDAVRLPPECDALRQEVRDFLAAEIAAGTYNPQEPGNDH
                     GFSPEFSRKVGAKGWIGMT"
     gene            complement(203..1306)
                     /locus_tag="VFR00_07065"
     CDS             complement(203..1306)
                     /locus_tag="VFR00_07065"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011439460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEU0159054.1"
                     /translation="MESTSLIAETASRLLRHHCDPQTLNNAAATTWQGAAWAAMEQAG
                     LPLAWVPEDLGGAGVGLEEGFGLIGLAGRYALPLPLTETLLAGWLLARGKIAPPPGAL
                     TCAPTRQGDKVVMASAGTLSGRLRAVPFAEAAQHLALIAAREDGSGPAIALVALADVR
                     LAPGRSIAGDALNTVSLEAVRPIRSEIAPVGFAANDLLAMGAAARAMQMSGALAAILE
                     IAVAYANERVAFARPIGKFQAVQHNLARLAGETAAAMAAAGSAAEALASAYAVPAGAT
                     RLPSESLADALFLEVAAAKIRVGEAAGVGAGIAHQVLGAIGFTREHVLHRFTQRLWAW
                     RDDFDNESAWAVRLGALIAARGSDGLWPLLASR"
     gene            1409..2107
                     /locus_tag="VFR00_07070"
     CDS             1409..2107
                     /locus_tag="VFR00_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011313403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU0159055.1"
                     /translation="MPASKTLLSWSTGKDAAWALHCLRQDAMIEVVGLVSTINAAAAR
                     VAMHGVRRELLDAQAAAAGLPLFVLPLPSPCPNQVYEDIMATFVASQVAAGVEAIAFG
                     DLFLADVRRYRENALAGSGMRAIFPLWGLPTAPLARAMIAAGLEAYISCVDPARLPAR
                     FAGRAFDLDLLAELPAGVDPCGENGEFHSFAWAGPMFDRRLAVAPGERVLRDGFIYCD
                     LLAQWPRAPAPEAT"
     gene            2104..3036
                     /locus_tag="VFR00_07075"
     CDS             2104..3036
                     /locus_tag="VFR00_07075"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HEU0159056.1"
                     /translation="MKSSEALRVVSLIASATEIIAALERGDWLVGRSHECDYPAEVSR
                     LPTLTAPKLKVDAASGEIDRQVREIVRDGLSVYRVDAERLRALAPDVIVTQDHCDVCA
                     VSLRDVEAATCHWTDRHVQIVSLRPDCLADAYADIGRVARALKAEAAGARLTAEIERR
                     LGALTVQPAQRRRPRVAFIEWVEPLMAGGNWMPELIEAAGGRNLFGAAGKPSSWMSWA
                     DFVAADPEVIVVAPCGFALQRCLEELPLLQGRPGWAEIDAVRQGRVYFADGNAYFNRP
                     GPRLADSAEILAEMLFGETRARRGREVAWVRAKA"
     gene            complement(3081..4022)
                     /locus_tag="VFR00_07080"
     CDS             complement(3081..4022)
                     /locus_tag="VFR00_07080"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009337187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DmsC/YnfH family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HEU0159057.1"
                     /translation="MHPAYSVILFTTASGAGYGLLVWLALAWLLALLPAGRAFGVATF
                     GLAFVLITAGLLASTAHLGRPERAWRAFSQWRTSWLSREGVLALATYMPAAVLALGWT
                     QASENHPALRLAALLTATSAIMTLFCTGMIYASLRTIRQWHQPLTAPLYIALGLASGA
                     VLLNLLARAFGFDAAWPTWLALATLLLAGLMKWAYWRTIDGELRTYTVEAATGLGALG
                     KVRPLEPPHTEPNYIMREMGYRIARKHAKRLRRGVGIFLFAVPLAGCCLALLPPLGLI
                     GSAAAALALAAGLLIERWLFFAEAEHIVMLYYGADIA"
     gene            complement(4193..4981)
                     /locus_tag="VFR00_07085"
     CDS             complement(4193..4981)
                     /locus_tag="VFR00_07085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009463056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEU0159058.1"
                     /translation="MTALPAGTNKQLGLVIDLDTCVGCQACATSCKEWNAGGYSAPLT
                     DQDPYGKDPHGAWLNRVHAFEVGEGGESRTVNFPRSCLHCEEPDCVTVCPTGASYKRA
                     EDGIVLVDPDTCIGCKLCSWACPYGAREYDYGHGVMKKCTLCIDRIYNDHLGPEDRVP
                     ACVRACPTGARHFGDLGDPQSAVSQLVSARGGFALLPEFGYRPVNKYLPPRPRRDARA
                     AAAAAAEAGVGQTAGASATAAAPGAGAGSTAERLFAWIDQMLSR"
     gene            complement(4978..7869)
                     /locus_tag="VFR00_07090"
     CDS             complement(4978..7869)
                     /locus_tag="VFR00_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015319318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin oxidoreductase family protein"
                     /protein_id="HEU0159059.1"
                     /translation="MRAAPEPDIALSVPPADEIKTTTCYMCACRCGIRVYLKDNKVRY
                     IEGDRAHPVNRGVLCGKGSAGIMQHYSPARLHAPLLRVGERGSGEFKQISWDEALALA
                     SGWLGEVRRKDPSKLAFFTGRDQSQALTSWWAMQFGTPNFAAHGGFCSVNMAAAGLYT
                     FGGSFWEFGETDWEHTRYFVLWGVAEDHASNPLKIELGKLKARGVKIVSVNPVRTGYS
                     AIADEWIGIRPGTDGLFAGALVHELLSTHQIDVDYLRRYTNAGWLVIAAPGAADDGLI
                     ARGNDGRPLVWDTRTGRLTSALEAEVSPAFSGSFELADKRQAVPVFQLLAQRFLGPEY
                     APENVARTTGIAAATVRRIAHELAQAAFADPLLLDIPWTDWAGRQHAKSLGRPVGMHA
                     MRGISAHANGFHTCRILHVLQILLGSIDCPGGFRYKTPYPKQTPPTLKPRGKPGDVKP
                     MQPLAGPHLGFPTGPEDLLIDAAGKPQRIDKAYSWEAPFAAHGLMHTVIANAARGDPY
                     PIDVLFLYMANMGWNSAMNTPATLAHLTAKDPASGDYKIPKIIYSDAYFSETVPYADL
                     ILPDTTYLERWDCISLLDRPISEPNAPTDAIRQPVLAPDRDVRPFQSVLIDLGARLKL
                     PGFATPDGAPKYPGGYQDYIVNHERRPGIGPLAGYRGKDGGAWGRGAPNPRQLEQYVA
                     DGCVHVHELPTHMRYFKHANRAFIDWAVDMGFRITPTPVIFQLYSEPMQKMRLAARGH
                     GPVLPPSEHRQRVETYFDPLPFWYAPFEDAAGAEPGFPLHAITQRPMAMYHSWGSQNA
                     WLRQIHSCNRLYLSRARAAEFGIGDEDWVWIESRCGRVKAQVKLMEGVNPDTVWTWNA
                     IGKRAGAWGLDPQGPEATRGFLLNHLIAELQPQRPDGYRYTNSDPITGQAAWYDLKVR
                     IAKVGADEPLATSPQFAPLKAPPEPALPEQPQQASPRDTAKAPSKEAKR"
     gene            complement(8052..8648)
                     /locus_tag="VFR00_07095"
     CDS             complement(8052..8648)
                     /locus_tag="VFR00_07095"
                     /inference="COORDINATES: protein motif:HMM:NF015674.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HEU0159060.1"
                     /translation="MTDQLSRADVAGALAEIRQQVAAHWGWFLVLGLVLIVAGCAAIA
                     FPLLSTVAVKIALGWVFLVGGILMMVHAFSASRWRGLVWEVLLGILYVVAGAYLAFFP
                     LTGIITLTILLAAMFLVEGALEVVMALRVRPHEGWGWLLLSGLIAVAVGLLIALGLPS
                     SAAWAIGLLAGINLISTGCGFLVLALAGRRANTAVAAG"
     gene            complement(8800..10212)
                     /locus_tag="VFR00_07100"
     CDS             complement(8800..10212)
                     /locus_tag="VFR00_07100"
                     /inference="COORDINATES: protein motif:HMM:NF028178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HEU0159061.1"
                     /translation="MESNTAKASLGTVLAGGLGVAVYAVTLQAGSHTDGRVIMATGNA
                     HYQQLAETLRPELERNGVRLEFRDLEGFATLKALTDDKSGVNAGFVKGGLVGSMQGRL
                     ASEKAKGRHVEYAKLQSLGRLFYEPIWVFTRADTPLQSLRDLDGKRIMVGTRDSGSRR
                     IATQLLRANGINIVRDDPKLINEELSANAAELLEGKADAALLILPADADKIQALLHVP
                     MIRLMDFSAEADAYDNRFPAITKLVMRTGSVEFKPLIPSADITLLATSVALVVRPDLD
                     ASLASLLTQALMNNPKPGFDKNGDPVLFYKPGEFPSPSDPEFEVPKEARQIYKTNELP
                     FLLRLLAPFNQRIGLPYNATAYASAHAAKLVLLIPALAVLVPLIRFLPIAYAWNVRRR
                     LLYWYRQLKALERSLDSGGAKYDLAAHEAEIERIDSHVRRLRVPLYFSSQLYDLRGHI
                     DLVRHRIAGRPPHNIRMAAE"
     gene            complement(10394..12349)
                     /locus_tag="VFR00_07105"
     CDS             complement(10394..12349)
                     /locus_tag="VFR00_07105"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEU0159062.1"
                     /translation="MPTTAPALPPLIPRAKLFGNPTRAQGQIAPNGRFLSWLAPHDGV
                     LNVWVAPMAAIGEARVITTDKKRGIRFYAWAYSSQHIIYLQDEGGSEDWHIYAVPIDG
                     GPGRDLTPFPGVSARIEQLSLDHPEALAVAINDRDKAWHDLYRIDINTAERQLLFENT
                     GQLGAVLTDRQLIPRLAVKPRGPEGGKVVYRIDGTRLEEMFVVEHEDDLTTQPIGFTH
                     DAATFYSISSIGRDKAALFATAWDSGSARLIAEHPKADISRVISHPVTGVIDAVGAKH
                     LALDWIALSEPMAADLKHLHGALPGEVSIADRSLDDRYWVVAASAAESPTTYHLYRRA
                     SGDISELFATQPELKSYTLARMRSSLIISRDGLELPSYYTLPAAEPLRPTEPLPLVLL
                     VHGGPWSRDEYGFNALHQWLANRGFAVLSVNFRASTGFGKAFVNAGDLEWGRKMHEDL
                     LDAVAWAVEEGIADKDRIAIMGGSYGGYATLAGLAFTPDVFCCGVDIVGPSNLETLLA
                     TIPPYWAAFFENLVRRVGDPRTEAGRQLLKARSPLYAAGNIKKPLLIGQGANDPRVKQ
                     SESDQIIAAMRAKALPVTYVLYPEEGHGFAEPANRIAFFAIAEAFLVQHLGGRAEPIG
                     GDFAGAKFEVPQGADAVPGLTAMRPTA"
     gene            12745..13962
                     /locus_tag="VFR00_07110"
     CDS             12745..13962
                     /locus_tag="VFR00_07110"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0159063.1"
                     /translation="MSAASQSPLRQRRNVALLALAQALFMSVQGMGISATPLAGYMLL
                     DDTEKWLATTPVFLVHLAIMGTTVPASLLMARIGRRAGFSVGAVAGVLAGGVGTLALL
                     RASFPLLCLSAVLQGMQAAFFWYFRLAAADAAEPPFRPKAISLVMAGGVLAGVLGPQT
                     AKLAVDWLQPVPFAGIYLAMAGFSVAVGLLVQLIRIPRLSAAERAAGGRPMTLIARQP
                     AYRVALAASVFGYAVMTLTMSATPLAMLACGFTFNDSATVIQAHIVGMFLPSFFTGHL
                     IQRFGVLPIIAVGGLIELGCALVNLAGVGFGNFLIANMLVGIGWNFAFVGGSSLLTTT
                     YTPSERAKVQASHDFTVYSATATAAAVSGVLAAKAGWAVINLAAVPLMVVVSGAALWL
                     MSQQRRTSAAAAK"
     gene            13972..14361
                     /locus_tag="VFR00_07115"
     CDS             13972..14361
                     /locus_tag="VFR00_07115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0159064.1"
                     /translation="MGDRYDRNRRRGLHPLVRALPKPPPEIIGWGLTAPARIGHNQGP
                     PLDEPVVDVFHRYRWRKAHAEVWKHPPLSILKWRMQRAEAAGVSYRAYMLALLDTGRH
                     LQAADVAKSQGEDATDKAAPRLRPDKP"
     gene            14358..14867
                     /locus_tag="VFR00_07120"
     CDS             14358..14867
                     /locus_tag="VFR00_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018459860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HEU0159065.1"
                     /translation="MSDPFDPERHRLAPSRYFEDFELGQTFALPSRTVTEAQFLAFQA
                     ASGDNHPIHYDREYCRRHGHPELLAHGYQVVIQTAAGAGLFPFLVEDSLVAFLEQSSR
                     FLAPVYAGDTLYPRLTVAELRPQRTTGVLSMATSIHNQRRKLVLEGVQKYLLRRRPMG
                     PGEAGVAAD"
     gene            15107..16042
                     /locus_tag="VFR00_07125"
     CDS             15107..16042
                     /locus_tag="VFR00_07125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0159066.1"
                     /translation="MPIIEPAPWRHQFFEHVVCPPDLFIPTDDPEAWEWNPRHRWVFD
                     KLQVALSQGLAAGPHGTCPPQYPVFSKPIMNFRGLGAGSRILMSADDYDRNHAPGHFW
                     MSLLEGRHVSTDVAVVAGQPCWWRHVTGLAVGEGMFDTWTVHAQHDAELEDYCGSWIR
                     GNLVGYTGMLNLETIGGRIIEAHLRLSDQWPDLYGPGWIDAFVGLYRDRIWTYADADR
                     RDGFSLVLCGPTGRQYPQPPRDVLQTVERMPGVSSVQITFHADKPPELHAMPPGGFRV
                     AVVNAFDRQAGEAAREVLRAFFLGAEPAVNIARKP"
     gene            16090..17262
                     /locus_tag="VFR00_07130"
     CDS             16090..17262
                     /locus_tag="VFR00_07130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0159067.1"
                     /translation="MRRHEGIAGLVCLALLLAPAAAASQTTAVELAAKLSPAQRQIYE
                     DYRRAREAFEAKLRAYWTRVDAKRDARRAKHMLAQALSADDYVPEQPPKYGGPELPAE
                     IAKIVGEVVPPVPARPLPDVSDFLAAAKAHYSFVPTATTELDFKRRYAREALAAGLSK
                     DQVLRIYALETGGQGTYDMQSGVNPVTKQGRPISSALGYAQLLNGNSVSELVKHGTSF
                     QQRLLKMAAEPGLPPARVAALREKAAVLRRMLRAARSVPNEWAAHVRLGGTVNGLGIH
                     ALNLDADVGPWLQVLKLKGLKEAAEIAGRGQLSGAEIELMNLAGPRTGLEMMTPLGRT
                     MPTANFFSEGGYYRNTIVREKTAAELLAALEQRMEASLKKPGAVEFAQVFDELARR"
     gene            17329..18693
                     /locus_tag="VFR00_07135"
     CDS             17329..18693
                     /locus_tag="VFR00_07135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEU0159068.1"
                     /translation="MNQSRNASARAVPFTQTIGGCLSTSIGEHGLTEAELKGWLARLT
                     APLVELKRSYRERTLPLLRIAEETADLEAAEQAYAKLVVGGRALVFFGIGGSSLGGQA
                     LAQLGGWSIPGTADEAQKARPRTRFYDNLDAHTLGAALAAFDLDTARFVVTSKSGGTP
                     ETLVQALAALDAVKRQGLERRIPELFLGITEPKQEGKANGLRQLFEAHGIALLDHHTG
                     IGGRFSVLTNVGLLPALARGLNVRALRAGAQSVIEAMLAAEAPQDCGPAVGAALAVAL
                     ARERGVRTLVMLPYADRLSRFAHWFVQLWAESLGKDGQGTTPIACLGPVDQHSQLQLF
                     MAGPREHLITVVRAPTASQGPRLSTDLAGVAGLEYLGGKTVGDLVAAQAIAVPEALGR
                     AGRPVRTFDLARLDEANLGALMMHLMLETILAGRLLGVDPFDQPAVELAKVITRERVA
                     ALKA"
     gene            18728..20548
                     /gene="mutL"
                     /locus_tag="VFR00_07140"
     CDS             18728..20548
                     /gene="mutL"
                     /locus_tag="VFR00_07140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018453471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEU0159069.1"
                     /translation="MPIRQLSAETINRIAAGEVIERPASVVKELVENAIDAEATAVEI
                     VTVAGGLSLIRVSDNGVGMTGSDLLLAVDRHATSKLDEEDLFNIATLGFRGEALPSIG
                     AIAHLALASRSRAANDAHEIVVDRGNKAALRPAAIARGTRVEVRELFSATPARLKFLK
                     SERAENFAINEVVKKIALAQPGIAFSLTTGERTALRLPQEAAGPAGHLSRLGRIMGQD
                     FLRDALPVEAEREGIRVTGFAGLPTLNRPDSGQQHLFVNGRPVRDRLLIGAVRAAYAD
                     LIPRGRSPLLALFVTLPAREVDVNVHPTKAEVRFRNVHGVRSLVLGGLRAALEAAGHR
                     ASDRGGTAMLAALAQSAGDGKAAPGVNFPVAPAAPAWSGRAYPRSSTATLAAMQAPLD
                     GMAAPSADWSAAAAPAAPELVDRPLGAARAQLHETYIVAQTRDAVIIVDQHAAHERLI
                     YERIKAQLADGGVARQALLIPVIIELDADEIAGLAEAGAALLELGLRLETFGPGAVMV
                     REIPALLGDADIGGLVKDLAREALRESAGALLNERLAAVCSSMACHGSVRAGRRLTGQ
                     EMNALLREMELTPHAGQCNHGRPTYVELKLADIERLFGRR"
     gene            20770..>21663
                     /locus_tag="VFR00_07145"
     CDS             20770..>21663
                     /locus_tag="VFR00_07145"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008835158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEU0159070.1"
                     /translation="MSFVVMKFGGTSVANLERIRNVARHVKREVDAGNHVAVVVSAMA
                     GVTNQLVAWVREASPLHDAREYDAVVATGEQVTAGLLAIVLQSMAIPARSWQGWQIPI
                     MTDDAHGVARIKDIPGDKIRARLEQGEVAVITGFQGIEPNRNRISTLGRGGSDTSAVA
                     LAVAVRADLCDIYTDVDGVFTTDPRIVPRARRLARVSYEEMLEMASLGSKVLQTRSVE
                     LAMVHRMPLRVLSSFMPPENLTPVDAQSWDEIGTIICSEDEIVEQHVVSGIAYAKDEA
                     KVSLIRVEDKPGVAARVFGPLA"
BASE COUNT         3602 a         7047 c         7365 g         3649 t
ORIGIN      
        1 caggtcatgc cgatccagcc cttggctccc accttgcggc tgaactccgg gctgaaacca
       61 tggtcgttgc cgggctcctg cggattgtaa gtgcccgcgg cgatttctgc cgcaaggaag
      121 tcgcgcacct cctggcgcaa ggcatcgcac tcgggcggta ggcggacggc atcgaagctc
      181 agcgcagtca tgccgggtct cctcagcgcg atgccagcag cggccatagt ccgtcgctgc
      241 cccgcgctgc aatcagcgcg ccgagccgca ccgcccaggc actctcgttg tcgaagtcat
      301 cccgccaggc ccacagccgc tgcgtgaaac gatgcagcac atgttcgcgc gtgaagccaa
      361 tcgctcccaa tacctgatgc gcgatgccag cacccacgcc tgccgcctcg ccgacccgga
      421 ttttggcggc tgccacctcc aggaacaagg catccgccaa gctttcgctg ggcagccgcg
      481 tcgccccggc gggcacggca taggcgctgg cgagagcctc cgcggcggag ccggccgcgg
      541 ccatggcggc ggcggtctcg ccggcgagac gggccaagtt gtgctgcacg gcttggaact
      601 tgccgatcgg gcgcgcgaaa gcaacacgct cgttggcgta ggcgacggcg atctccagga
      661 tggccgccag cgccccgctc atctgcatgg cgcgcgccgc agcacccatg gcgagaaggt
      721 cgttggcagc gaagccgacc ggcgctattt cgctcctgat gggtcgcacc gcttccaagc
      781 tcaccgtgtt aagggcatcc ccagcgatgc tgcgaccggg ggccaggcgc acgtcagcga
      841 gcgcgaccag ggctatggca gggcccgacc cgtcctcacg cgcggcgatc aaggccaagt
      901 gctgggccgc ttcggcaaag ggaaccgcgc gcagccgacc gctgagggtc ccggcggagg
      961 ccatgacgac cttgtcgcct tgtcgtgttg gagcgcacgt tagcgctccc ggtggcggcg
     1021 caatcttgcc gcgtgccagc agccaacccg ccaacagcgt ctcggtgagc ggcaggggca
     1081 acgcgtaacg gccggccagg ccgattaggc cgaagccctc ctcgaggccg acgccggcgc
     1141 cgcccaggtc ttccggcacc caagccagcg gcagacctgc ctgctccatg gcggcccacg
     1201 ccgcgccctg ccaagtcgtc gcggcggcgt tgttgagcgt ctgggggtcg cagtgatgcc
     1261 gcagcaggcg ggaggccgtc tcggcgatga ggctcgtgct ctccatgctt gcgcccctcg
     1321 gcatgctata aggaaggcaa gctttggcag cggcgcgcgg caacgtcaac tgcgcgccgc
     1381 agcatttgcg ccagcttggg agcaccgcat gccggccagc aaaacccttc tgagctggag
     1441 cacgggcaag gacgcggcct gggcgctgca ctgcttgcgg caagatgcga tgatcgaggt
     1501 ggtcgggctg gtgtcgacca tcaatgctgc ggccgcgcgc gttgccatgc acggcgtgcg
     1561 ccgcgagctg ctcgacgcgc aggccgccgc tgccggtctg cccctgttcg ttttgcctct
     1621 gccctcgccc tgcccgaacc aggtctatga ggacatcatg gcgacattcg ttgccagcca
     1681 ggtggccgcg ggcgttgagg cgatcgcgtt cggcgatctc tttcttgccg atgtccgccg
     1741 ctaccgcgag aacgcgctcg ccggcagcgg catgcgcgca atctttccgc tctgggggct
     1801 gccgaccgca ccgcttgccc gcgccatgat tgctgccggc ctcgaggcct acatcagctg
     1861 tgtcgacccc gctcggctgc cggcacgctt tgccgggcgc gctttcgatc tcgacctgtt
     1921 ggccgagttg ccggccggcg tcgatccttg tggcgagaac ggtgaattcc acagctttgc
     1981 ctgggccggg cctatgttcg accggcgcct cgccgtcgcg ccgggcgagc gcgtgctgcg
     2041 cgacggcttc atctactgcg acctcttggc ccaatggccg cgcgcgccgg cgccggaggc
     2101 gacatgaagt caagcgaagc cttgcgggtc gtcagtttga ttgccagtgc gacggagata
     2161 atcgccgcgc tcgaacgtgg tgactggctc gtcgggcgta gccacgagtg cgactaccct
     2221 gccgaggtca gccgtttgcc gacgctgacg gcgccgaagc tcaaggtcga tgctgccagc
     2281 ggcgagatcg accgccaagt gcgcgagatt gtccgcgacg gcctctccgt ctatcgggtc
     2341 gatgccgaac ggctgcgcgc gctcgccccc gacgtgattg ttacccaaga tcactgcgat
     2401 gtctgcgccg tcagcttacg cgatgtggag gcagccacct gccattggac cgaccgccac
     2461 gtccagatcg tctccttacg acccgattgc ctggccgatg cctatgccga tatcggccgc
     2521 gtcgcccggg cgctcaaggc ggaggcggca ggcgcccgcc tcacggccga gatcgagcgg
     2581 cgcttgggcg ccttgacggt tcagcccgcg cagcgccgcc gaccgcgggt cgccttcatc
     2641 gagtgggtcg aaccgttgat ggcgggcggc aactggatgc ccgagctgat cgaagctgcg
     2701 gggggacgca acctattcgg tgccgccggc aaaccatcga gctggatgtc ctgggccgac
     2761 ttcgtcgccg ccgatccgga ggtgatcgtc gtggcgccgt gcggctttgc cctgcaacgt
     2821 tgcctggagg agctgccctt gttgcagggc aggccgggtt gggccgagat cgatgccgtg
     2881 cgccagggcc gtgtttattt tgccgacggc aatgcctact tcaatcgccc cgggccgcgg
     2941 ctggcggaca gtgccgagat cctggccgaa atgcttttcg gcgagacgcg cgcgcggcgc
     3001 ggtcgagagg tcgcctgggt cagggccaag gcttgaggcc gcccggcctc gcgtggcatc
     3061 cccgggacgt gcgtgccgcc tcaagcgatg tcggcaccgt aatagagcat gacaatgtgc
     3121 tcggcctcgg caaaaaacag ccaccgctcg atgaggagcc ccgccgccag cgcgagcgct
     3181 gcagcggccg agccgatgag cccgaggggc ggcagcaaag ccaggcagca gccggcaagc
     3241 ggcaccgcga acagaaaaat cccgacccct cggcgcaatc gcttggcatg cttgcgggcg
     3301 atccgatagc ccatctcgcg catgatgtaa ttgggctccg tgtggggcgg ctcgaggggc
     3361 cgcaccttgc cgagcgcacc cagaccggtg gccgcttcta ccgtgtaggt tctgagctcg
     3421 ccgtcgatcg tgcgccagta ggcccacttc atgaggccgg cgaggagcag ggtggcaagc
     3481 gcgagccagg tgggccaggc ggcgtcgaac ccgaaggcgc gcgcaaggag gttgagcaac
     3541 acggcgccgc tggcaaggcc caaggcgata tagaggggcg ccgtcaaggg ctgatgccat
     3601 tgtcggatgg tacgcagcga ggcgtagatc atgccggtgc aaaaaagcgt catgatcgcg
     3661 ctcgtcgccg tcaacagcgc ggcgagccgt aaggccggat ggttctctga tgcctgggtc
     3721 cagccgagcg ccaggacagc tgcgggcata taggtggcca aggccagcac gccctcccgc
     3781 gacagccatg aggtgcgcca ctgcgagaag gcgcgccagg cgcgctcggg ccggccaaga
     3841 tgggcggtgg acgctagcag gccggcagtg atgagcacaa acgcaaggcc gaaggtggcc
     3901 acgccgaagg cgcgcccagc cggtagcagc gccaggagcc aggccaacgc cagccaaacg
     3961 agcaagccgt agcccgcccc cgaagcggtc gtgaacagga taacggagta ggcggggtgc
     4021 atggcgtggg tgcctaggaa gtccgttgca gcacgcgctc cacgtcgccc tcaccgtgcg
     4081 gggacgaggt ctgggcgcgg ctggcggcga acgggcgaac ccatcgcctg cgggcgttgg
     4141 cagggtgtct catcgcaagc ggggacatgt gcgctggccg gtccacgacg cttcaccgcg
     4201 aaagcatctg atcgatccag gcgaatagcc gctccgcggt gctccccgcg ccggcgcccg
     4261 gtgccgccgc ggtcgcactc gcgccggccg tctgacccac ccccgcctcc gccgccgcgg
     4321 cggcggctgc tcgggcgtcg cgccgcgggc gtggtggcag atacttgttg acgggccggt
     4381 agccgaactc gggcaacagc gcaaagccgc cgcgcgcgct cacgagctgc gacacggcgc
     4441 tttgcgggtc accaaggtcc ccgaaatgac gcgctccggt cgggcaggcg cgcacgcagg
     4501 cgggtacgcg gtcctcgggg ccgagatgat cattgtaaat gcggtcgata catagcgtgc
     4561 acttcttcat gacgccgtgg ccgtagtcgt attcgcgtgc tccataaggg caggcccacg
     4621 agcacaactt gcagccgata caggtgtcgg ggtcaaccag gacgatgccg tcttccgcgc
     4681 gcttgtagga ggcgcccgtc gggcagaccg tgacacagtc gggttcctcg cagtgcaggc
     4741 acgagcgggg aaaatttacg gtcctgctct cgccaccttc gccgacctcg aaagcgtgga
     4801 cgcgattgag ccaggcgccg tgcggatctt ttccgtaggg atcctgatcg gtcagcggtg
     4861 ccgagtagcc gccagcattc cactccttgc agctggtcgc acaggcctga cagccgacgc
     4921 aggtgtcgag atcgataacc aagcccaatt gcttgttcgt cccagcaggg agcgcggtca
     4981 tcgcttggct tccttgctcg gcgccttcgc cgtgtcgcgc ggcgacgcct gttggggctg
     5041 ttcggggagc gccggttccg gtggcgcctt gagcggggcg aactggggcg acgttgccag
     5101 cggctcgtcg gcgccaacct tggcgatgcg gaccttgagg tcgtaccagg ccgcctggcc
     5161 ggtaatgggg tcgctgttgg tatagcggta gccatccggc cgttgcggct gcagttcggc
     5221 aatcaggtgg ttgagcagga agccacgcgt ggcttccggt ccttgtgggt cgaggcccca
     5281 ggcgccggcg cgcttgccga tggcattcca ggtccagacc gtatcaggat tgaccccctc
     5341 catcagtttg acctgcgcct tgacgcggcc gcagcggctc tcgatccaca cccaatcctc
     5401 atcgccgatg ccgaactcgg cggcgcgggc acggctcaaa tagagacggt tgcagctgtg
     5461 gatctgccgc agccaggcgt tctgcgaacc ccatgaatgg tacatcgcca tcggccgctg
     5521 ggtaatggcg tgcagcggaa agccgggctc ggcgccagcc gcatcctcga agggcgcgta
     5581 ccaaaagggc aatgggtcga agtaggtctc gacgcgctgg cgatgctcgc tgggaggtag
     5641 cacgggaccg tgccctcgcg ctgctaggcg catcttctgc atcggctcgc tatagagctg
     5701 gaaaatgacc ggcgtcggcg tgatgcgaaa tcccatgtcg accgcccagt cgatgaaggc
     5761 gcgattggca tgcttgaagt agcgcatgtg ggtcggcagc tcgtgtacgt gcacgcaacc
     5821 atcggcgacg tactgttcga gctggcgcgg gttcggcgcg ccgcggcccc aagcgccgcc
     5881 gtccttgcca cgataaccgg ccaacggacc aatgccgggc cggcgttcgt ggttgacgat
     5941 atagtcctga tagcccccag ggtatttcgg tgcaccatca ggcgtagcga agcccggcag
     6001 cttgaggcgt gcgccaagat cgatcagcac gctttggaac ggccgcacgt cgcgatcggg
     6061 tgcaagcacc ggctggcgaa tggcgtcggt gggcgcgttc ggctcggaaa tcggtcgatc
     6121 gagcagcgag atgcagtccc agcgctcgag gtaggtcgta tcgggaagaa tgaggtcggc
     6181 gtagggtacc gtctcggaga agtaggcgtc cgagtagatg attttgggta tcttatagtc
     6241 gccgctggcg gggtccttgg cggtgagatg cgccaaggtg gccggtgtgt tcatggccga
     6301 gttccagccc atgttggcca tgtagagaaa gagcacgtcg atgggatagg gatcgccgcg
     6361 cgcggcattg gcgatcaccg tgtgcatgag cccatgcgcc gcaaagggtg cctcccagct
     6421 ataggccttg tcgatgcgct gcggcttgcc cgccgcgtcg atcagcaagt cctcaggacc
     6481 cgtgggaaag ccaaggtgtg ggcccgccaa aggttgcatc ggcttcacgt cgccgggctt
     6541 gcccctcggc ttgagcgtgg gcggcgtctg cttcgggtag ggcgtcttgt aacgaaagcc
     6601 gccgggacaa tcgatcgagc ccaacaggat ctgcaagacg tgcagaatgc ggcaggtgtg
     6661 gaagccgttg gcgtgggcgg agatgccgcg catggcgtgc atgcccaccg gccggccgag
     6721 gctcttggcg tgctgacggc cggcccagtc ggtccaggga atgtcgagca ggagggggtc
     6781 ggcgaaggcc gcctgcgcca gctcatgcgc gatgcggcgg accgtcgccg ccgcaatgcc
     6841 cgttgtgcgg gcaacatttt ccggcgcgta ctcggggccc agaaaacgct gagccaaaag
     6901 ctggaacacc gggacagcct ggcgcttgtc ggcaagctcg aagctgccgg aaaaggccgg
     6961 tgacacctcc gcctcgagcg cgctcgtaag gcggccggtc cgcgtgtccc aaacgagcgg
     7021 ccggccgtcg ttaccgcggg caatcaagcc gtcgtcggcg gcgccgggcg ccgcaatcac
     7081 gagccagccc gcattggtgt agcgccgaag ataatccaca tcgatctggt gcgtactgag
     7141 aagctcatgc accagcgcgc cggcgaaaag gccgtcggtg ccgggcctaa tcccgatcca
     7201 ttcgtccgca atcgcgctgt agccggttcg cacggggttg acgctcacaa tcttgacgcc
     7261 ccgcgctttc agcttgccga gctcgatctt gagcggattg gaggcgtggt cctcggccac
     7321 gccccagagc acgaagtagc gagtgtgctc ccagtcggtc tcgccgaact cccaaaagga
     7381 gccgccaaag gtgtaaaggc cggcagccgc catgttgacc gaacagaagc ccccatgggc
     7441 ggcgaagttc ggggtcccga actgcatggc ccaccagctc gtcagcgctt ggctctgatc
     7501 gcggccggtg aagaaggcga gcttgctcgg gtccttgcgc cgcacctcgc caagccagcc
     7561 gctggcgagc gccagcgcct cgtcccagga gatttgcttg aactcgccgg agccgcgctc
     7621 gccaacgcgc agcagcggtg cgtggaggcg tgccggcgag tagtgctgca tgatgccggc
     7681 ggaacctttg ccgcacaaga cgccgcggtt caccggatgc gcgcgatcgc cctcgatata
     7741 gcgaacctta ttatctttca ggtagacgcg aatgccacag cggcacgcgc acatgtagca
     7801 cgtggtggtc ttgatctcgt cggcaggcgg aaccgacaag gcgatgtcgg gctcgggggc
     7861 ggcgcgcaag cattgcctcg taaatgacgg cctcacgggg ccagcacgaa aagcgcgtgc
     7921 gcggctgacc ggccgggacg atacggccgg atcggcgcgc cgcgaaatgc ttttctcggc
     7981 gaatgggcgc agcccgccta cgccctattg ccgcacgccc cggacctgat gcaaggcgcg
     8041 aggctgcggc gttagccggc ggcaacggcc gtattggccc gccggcccgc cagcgccaga
     8101 accaaaaagc cgcagcccgt ggagatcagg ttgatgccgg ccaggagacc gatcgcccag
     8161 gcggccgaac ttgggagtcc gagcgcgatc aggaggccga cggccacggc gatgagaccg
     8221 ctcagcaaaa gccagcccca gccctcatgc gggcgcacgc gcagcgccat gaccacctca
     8281 agggcgcctt ccaccaggaa catggcggcg agtaaaatcg taagtgtgat gatgccagtc
     8341 agcgggaaga acgccagata ggcaccggcg accacgtaaa ggatgccaag caacacctcc
     8401 caaacgagac cgcgccagcg ggaggcggag aaggcatgca ccatcatcaa gatgccgccg
     8461 accaggaaca cccagccgag cgcaattttg acggcgaccg tggagagcag aggaaaggcg
     8521 atggccgcgc agcccgccac gatcaacacc agtccgagca ccaggaacca cccccaatgc
     8581 gcggcaacct gttggcgaat ttccgccagg gcaccggcaa catccgcacg cgagagctga
     8641 tcagtcatga gccgaacctc cgctggggtg ggcacgaggt tgcctcgcgc cgtttccgag
     8701 ccgcgccgat cctagcagga aaagcggaac cacgccgaac cgtcaggccg gttactgaca
     8761 gcaaactgga gcgttgttgc ccgaggtttt gcggcggcgt tattcggcgg ccatgcgaat
     8821 gttgtgcggt ggccgcccgg cgatgcgatg tcgcacgaga tcgatgtgac cgcgcaagtc
     8881 atagagttgg ctcgaaaaat agagcggcac gcgcagccgg cgcacatggc tgtcgatgcg
     8941 ctcgatctcg gcttcgtgcg cggccaaatc gtacttggcg ccgccgctgt cgaggctcct
     9001 ctccaacgcc ttcaattgcc ggtaccaata gagcaggcgc cggcgcacgt tccaggcgta
     9061 ggcgatcggc aaaaagcgga tgagcggcac cagcaccgcc aaggccggaa tcagcaacac
     9121 gagcttggcg gcatgcgcgc tggcgtaggc ggtagcattg tagggcaggc cgatgcgctg
     9181 gttgaacggg gcaagcaagc gcagcagaaa cggcaactca ttggttttgt agatctggcg
     9241 cgcctccttg ggcacctcga attcgggatc gctcggggac ggaaattcgc cgggcttgta
     9301 gaacaagacc ggatcgccgt ttttatcgaa gccgggtttg gggttgttca tgagggcctg
     9361 cgtaagcagg cttgccaagg aggcatcgag gtcgggtcgc accaccaggg cgaccgaggt
     9421 cgccagtaag gtgatatccg ccgacgggat caggggcttg aactcgaccg aacccgtgcg
     9481 catcacgagc ttggtgatgg cgggaaagcg gttgtcgtaa gcgtcggcct cggctgagaa
     9541 atccatgagg cgaatcatcg gcacgtgcag gagtgcctgg atcttatcgg cgtcggcggg
     9601 caggatcagc aaggcagcgt cggccttgcc ctcaagcagc tcggcggcgt tggcggagag
     9661 ctcctcgttg atgagcttgg gatcgtcgcg cacaatattg atgccgttgg cgcgcaggag
     9721 ctgggtggcg atgcggcgcg aaccgctgtc gcgggtgccg accatgatgc gcttgccgtc
     9781 gaggtctctg aggctctgca gcggcgtatc ggcgcgagtg aacacccaga tgggctcata
     9841 gaacagccga cccagggact gcagcttggc gtactcgacg tggcgtcctt tggccttttc
     9901 gctggcgagc cgcccttgca tgctgccgac cagtccacct ttgacgaacc ccgcgttgac
     9961 ccccgacttg tcgtcggtga gcgccttcag cgtggcaaag ccttcaaggt cccgaaattc
    10021 caggcggaca ccattgcgct ccaactcggg gcgcaaggtt tcggcgagct gttggtagtg
    10081 ggcattgccg gtcgccatga tgacgcgccc gtcggtatgc gagccggcct gcagggtgac
    10141 cgcatagacc gcgacgccaa gccccccggc caggacggtg ccgagcgagg ccttggccgt
    10201 gttggattcc atcgcgcacc ccccaacctt ccccgcaggc ccgcgcctga atgcccagtt
    10261 ctgtcaaatt tcccaagagt gtagtggact gttaaccatc ttgccacagc tcgatggcac
    10321 gcgtgtggca ggggcagccc gccacccacc gatcccgagg gcgggtcggt aactcgcctc
    10381 cgggcgcgcg cgcctaggcc gtcggccgca tcgcggtcag gccgggcacc gcatcggccc
    10441 cctgcggcac ctcgaattta gcgcccgcga aatcgccgcc aatgggctcg gcgcggccgc
    10501 ccaggtgttg cacgagaaag gcctcggcga tggcgaaaaa ggcaatgcgg ttggcaggct
    10561 cggcgaagcc gtggccttcc tctgggtaaa gcacgtacgt caccggcagc gccttggcgc
    10621 gcatggcggc gatgatttgg tcggattcgg actgcttcac gcgtgggtca ttggcgccct
    10681 gaccgatgag caacggcttc ttgatgttgc cggccgcgta gagcggcgag cgcgctttta
    10741 ggagctggcg gcccgcttcg gtgcgcggat cgccgacgcg acgaaccagg ttctcaaaaa
    10801 aagccgccca gtacggtggt atcgtcgcga gcagggtttc caagtttgag gggccaacaa
    10861 tatcgacgcc acaacagaag acgtcggggg tgaaggcgag gccggccagc gtggcatagc
    10921 cgccgtagga accccccatg atggcgatac gatccttgtc ggcgatcccc tcctccactg
    10981 cccaggccac tgcgtcgagc aagtcctcgt gcatcttgcg gccccactcc agatcgccgg
    11041 cattgacaaa ggccttgccg aaacccgtgg aggcacgaaa gttgaccgac agcacggcaa
    11101 agccgcggtt ggcgagccac tggtgcaggg cattgaagcc gtattcgtcg cgcgaccacg
    11161 gccctccatg cacgagcagc accaatggga gcggctcggt cggtcgcaac ggctcggccg
    11221 cgggcagcgt gtaataggag ggcagctcga gcccgtcgcg ggagatgatc agcgagctgc
    11281 gcatgcgcgc caaggtatag cttttgagct cgggttgggt ggcaaagagt tcgctgatgt
    11341 cgccgctcgc gcgccggtag aggtgatacg tcgtcgggct ctccgccgcg ctcgccgcca
    11401 ccacccagta gcgatcatcg agcgagcggt cggcaatcga cacttcgcca ggcaaggcgc
    11461 cgtgcagatg tttcagatcg gccgccatgg gttcgctgag cgcaatccag tccagcgcca
    11521 ggtgtttggc cccgaccgcg tcgatgacgc cagtaaccgg atgcgagatg acgcggctga
    11581 tatcggcctt gggatgctcg gcgatcaagc gcgcgctgcc gctgtcccag gcggtggcga
    11641 acagcgcggc cttgtcgcgc ccgatcgagg aaatactgta aaacgtggcg gcgtcgtgcg
    11701 tgaagccgat cggctgcgtc gtgagatcat cttcgtgctc gacgacgaac atttcctcga
    11761 gccgggtgcc gtcgatgcgg tagacgacct tgccgccctc cggcccgcgt ggcttgacgg
    11821 cgaggcgggg gataagctgt cgatcggtga gaacggcccc gagctggccg gtattttcaa
    11881 acagcagttg ccgctccgcc gtgttgatgt cgatgcgata gagatcgtgc caggccttgt
    11941 cccggtcgtt gatggccacg gccaacgcct cgggatgatc gaggctcagc tgttcgatgc
    12001 gggcgctgac gccagggaag ggcgtgagat cccgtccggg accgccatca atgggcaccg
    12061 cgtagatgtg ccagtcctcg ctgccgccct cgtcctgcag gtaaataatg tgctggctgc
    12121 tataggccca ggcgtagaag cggatgccgc gtttcttgtc cgtggtgatg acgcgcgcct
    12181 cgccgatggc cgccatcggc gccacccaga cgttgagcac gccgtcgtgt ggcgccagcc
    12241 atgacaggaa gcgaccgttg ggtgcgatct ggccttgcgc ccgcgtcgga ttgccgaaca
    12301 gtttcgcgcg cgggatgagg ggcggcaggg ctggggcggt tgtcggcatg ggttggctcc
    12361 tcggccttgg ctcgaacttg cgagatgggg gcgccggcgg tccccctgca agtgggcgcc
    12421 aagccagcac gcctggcgag gacggcattt tcaaggtcac cgcgctcgcc tttgcggcgg
    12481 gccggccgca aacttgaggc gtgaccggcc ggcacgcaac cggcacacga cgtttgctcg
    12541 ccgaggtcgt tggcgaccgg gcaacggccc tcttggcgct cgcacgggct tggcaaattg
    12601 gccgattttc tctgcgctgg ccgcaaggct cactgccgca aaaacgggaa gacagcgccg
    12661 cgcgcccacg atatggagac aaaccgagcc cgccggctgc tccgtcgcct ctcccgttgg
    12721 cctctcgatc ttcaccgcaa gcccatgagc gcagcgtccc agtcccccct ccgccagcgc
    12781 cgcaacgtgg cgctgttggc gctggcccag gcgcttttca tgtcggtgca aggtatgggg
    12841 atttccgcca cgccgttggc cggctacatg ctgcttgacg acacggagaa atggctggca
    12901 acgacgccgg tcttcctggt gcaccttgcc atcatgggca ccacggtgcc ggcctcgctg
    12961 ttaatggcca ggatcggtcg ccgcgccggt ttttccgtgg gcgccgtggc gggggtgttg
    13021 gctgggggcg tcggcacgct ggcgctgttg cgcgcgagct tcccgttgct ttgcctctcc
    13081 gcggtgctgc agggcatgca agcggcgttc ttctggtatt tccggctcgc tgcggccgac
    13141 gcggccgagc ctccgtttcg acccaaggcc atctccttgg tgatggccgg cggtgtgctc
    13201 gccggcgtgc tcgggccaca aacggccaaa ctggcggtgg attggctgca gccggtgcca
    13261 tttgccggca tttatctggc catggcgggg ttttccgtgg ccgtcggcct cctcgtgcag
    13321 ttgatccgca tcccgcgcct cagcgccgcc gagcgtgccg cgggcggccg ccccatgacc
    13381 ctgattgcgc gccagccggc ctaccgcgtg gcgttggccg cctccgtctt cggctacgcg
    13441 gtcatgacgc tcaccatgtc ggcgacgccg ttggcgatgc tggcctgcgg ctttaccttt
    13501 aacgacagcg ccacggtgat ccaggcgcat atcgtcggca tgttcctgcc gtcattcttc
    13561 accggtcatc tgatccagcg cttcggcgtg ttgccgatca ttgctgtggg cgggctcatt
    13621 gagcttggct gcgccctagt caacctcgcc ggcgtcggct ttggcaattt cctcatcgcc
    13681 aacatgctcg tgggcatcgg ctggaacttc gctttcgttg gcggatcctc gttgctcacg
    13741 accacctaca cgccgagcga acgtgccaag gtccaggcga gccacgactt cacggtctat
    13801 agtgccacgg caaccgcggc cgccgtttcc ggcgtcttgg cggccaaggc cggttgggcg
    13861 gtcatcaacc tggcggcagt tcccctgatg gttgtggtga gcggcgcagc cctgtggctg
    13921 atgagccagc agcgtcgtac gtccgcggcg gcggcaaaat agggcagcgc catgggcgac
    13981 cgctatgaca gaaatcggcg gcgcgggctt catccactgg tgcgcgcgct accgaagccg
    14041 cccccggaga tcatcggctg ggggctgacc gcgccagcgc gaatcggcca caatcaaggc
    14101 ccgccactgg atgagcccgt cgttgacgtc ttccaccgct accgttggcg caaggcgcac
    14161 gcggaagtct ggaagcatcc gccgctctcg atcctgaagt ggcgcatgca gagggccgag
    14221 gccgccgggg tcagctatcg cgcgtatatg ctcgccttgt tggataccgg gcggcatctg
    14281 caagccgccg acgtcgccaa atctcaaggc gaggacgcaa cggacaaggc ggcgccccgc
    14341 ttgcggcccg acaagccatg agcgatccgt tcgatccgga acggcaccgg ttggcgccca
    14401 gccgctactt cgaagacttt gagctcgggc aaacctttgc gttgccctcg cgcaccgtga
    14461 ccgaggcgca gtttctggcc ttccaggcgg cctcgggcga caatcatccc atccactacg
    14521 atcgcgagta ttgccggcgc catggccatc cggagctgct tgcccacggc taccaggtgg
    14581 tgatccagac cgcggcgggc gcgggcctct ttccgttttt ggtggaggac tcgctggtgg
    14641 cgtttttgga acaatcctcg cgctttctcg cccccgttta tgccggcgat acgctgtacc
    14701 cgcgcttgac cgtggccgag ctccgcccgc agcgcacgac cggcgttttg agcatggcga
    14761 ccagcattca caaccagcga cgcaagctgg tgctggaagg cgtgcaaaaa tatctgctgc
    14821 gacggcgtcc catgggcccc ggggaggcgg gcgtcgcagc ggactaacgg ggcggctgtt
    14881 tcacgtggaa caggatgcgg ccgcacaggc ccgtgtggcg cctgtctggc tttgcgcgtg
    14941 cctgttggag cgccgcgcgg catgcccgcg cccgaaggga cgcgctagtc gcgggttggg
    15001 cccccccggc ctcgatcctg cggatcgatg ataaacctgt tcgcgccggc tggcgccttg
    15061 gcctaagatt gcacccgcca gacaagaaca gcggacccat cgagctgtgc ccatcatcga
    15121 gccggcgccc tggcgccatc agttctttga gcacgtcgtt tgtccccccg acctcttcat
    15181 cccgaccgac gacccggaag cctgggaatg gaatccgcgg catcgctggg ttttcgacaa
    15241 gctgcaggta gcgctcagtc agggcttagc ggcgggtcca cacgggacct gtccaccgca
    15301 gtatcccgtc ttttcaaagc cgatcatgaa tttccgcggc cttggtgcgg gcagccgcat
    15361 tctcatgtcc gccgacgact atgatcgcaa ccatgcgccg gggcattttt ggatgagcct
    15421 gctcgagggc cgccacgtct ccaccgacgt cgctgtggtg gcggggcagc cctgctggtg
    15481 gcgacatgtc accggcctcg ccgtgggcga ggggatgttc gatacctgga cggtgcacgc
    15541 ccagcacgat gccgagctcg aagactattg tggcagctgg atccgtggca atctcgtcgg
    15601 ctacacgggc atgctcaacc tcgaaaccat cggcgggcgt atcatcgagg cgcacttgcg
    15661 cctctccgac caatggcccg acctttatgg gcccggctgg atcgatgctt tcgtcggcct
    15721 ctatcgtgac cgaatttgga cctatgcgga cgccgatcgg cgcgatgggt ttagcctggt
    15781 gctgtgcggt cccacgggac ggcaatatcc gcagccgccg cgtgacgtgc tgcaaacggt
    15841 ggagcgaatg ccgggtgtgt ccagcgtgca aatcactttt catgcggaca agccgccgga
    15901 gctgcatgcc atgccaccgg gcggcttccg cgttgccgtc gtcaacgcgt ttgatcgcca
    15961 ggccggggag gcggcgcgcg aagtgctacg ggcgtttttc cttggcgcgg aaccggcggt
    16021 caatatcgcg cgcaagccgt agggcctcgc cggctccagc actcccctcc agctaaggat
    16081 caaagcatcg tgagacggca tgagggaatc gcgggcctcg tttgcctcgc cctgctgctc
    16141 gcccccgccg ccgcggccag ccagacgacg gccgtcgagc tggccgccaa gctaagcccg
    16201 gcccagcgtc agatctatga ggattatcgc cgcgcgcgcg aggccttcga ggccaagctg
    16261 cgcgcctatt ggacgcgcgt cgatgccaag cgcgatgcgc gccgagccaa gcacatgctg
    16321 gcccaagcat tgagcgccga cgactacgtg cccgagcagc cccccaaata tggcgggccc
    16381 gagcttcccg ccgagatcgc caaaatcgtt ggcgaggtgg tgccgccggt gccggcgcgg
    16441 ccgctgccgg acgtcagcga ttttctggcc gcggccaagg cccactatag cttcgtgccg
    16501 acggcgacga cggagttgga cttcaagcgg cgctatgcgc gcgaggcgct ggcggccggc
    16561 ctcagcaagg accaggtgct ccggatctac gcgctcgaga ccggcggtca ggggacctat
    16621 gacatgcaat ccggcgtcaa tcccgtgacc aaacagggtc gtccgatttc ctccgcgctt
    16681 ggctacgccc agcttctcaa tggcaattcg gtaagcgagc tcgtcaagca tggcaccagc
    16741 ttccagcagc gattgctcaa aatggccgcc gagccaggtt tgcctcccgc ccgggtggcg
    16801 gcgctgcgcg agaaggcggc ggtccttcgc cgcatgctgc gtgccgcgcg cagcgttccc
    16861 aacgaatggg ccgcgcacgt gcgactgggc ggcacggtca acgggcttgg cattcacgcc
    16921 ctcaatctgg acgccgatgt ggggccctgg ttgcaggtcc tgaaactcaa gggcctcaag
    16981 gaggccgccg agatcgccgg ccggggccag ttgagcggcg cggaaatcga gctcatgaac
    17041 ctcgccggtc cgcgcaccgg tctggaaatg atgacacctt tggggcggac catgccgacg
    17101 gccaatttct tcagcgaagg cggatattac cgcaacacta tcgtgcgcga gaaaaccgcc
    17161 gccgagctat tggccgccct cgagcagcgc atggaagcca gtctgaagaa acctggcgcg
    17221 gtcgagttcg cgcaggtctt cgacgagctg gcacggcgct agccggcagc gccgccgccg
    17281 caagaggccg cgcttccctc gccgagaaat tcgcgctagg cctcgcccat gaaccaaagc
    17341 cgaaacgctt ccgcccgcgc cgtacccttc acgcagacga ttggagggtg cctgtcgacg
    17401 agcattggcg agcatggctt gacggaagcc gagctcaagg gctggctggc ccggctgacg
    17461 gcgcccctgg ttgaactcaa gcgcagctat cgcgagcgta cgctgccgct gttgcgcatt
    17521 gccgaggaga cggccgatct ggaggccgcc gagcaggcct atgcgaaatt ggtcgtcggc
    17581 gggcgcgcgc tcgtgttttt cgggatcggc ggctcgagcc ttggcggcca ggcgctcgcc
    17641 caacttggcg gttggagtat tcccggaacc gccgatgagg cacagaaggc gcgcccacgc
    17701 acgcgatttt acgacaacct tgatgctcat acgctgggcg cggcgcttgc cgccttcgat
    17761 ctggacaccg cccgctttgt ggtcacctcg aaatccggtg gcacgccgga gaccctggtg
    17821 caggcgctgg ccgcgctcga tgccgtgaaa cggcaaggtc tcgagcggcg catccccgag
    17881 ttgttcttag ggatcaccga gcccaagcag gaaggcaagg ccaacggcct gcgccagctg
    17941 ttcgaggccc atggcattgc gctcctcgac caccacacgg gcatcggcgg gcgcttctcc
    18001 gttctcacca acgtaggtct gctgcccgcc ctggcgcgcg gcctgaacgt tcgcgcgctg
    18061 cgggccggcg cccagagcgt gattgaggcg atgctggccg ctgaggcgcc gcaagactgc
    18121 ggcccggccg tgggagcggc tttggccgta gcactggcgc gtgagcgcgg cgtgcgcacg
    18181 ctggtgatgc tgccctatgc ggatcggctt agccgcttcg cgcattggtt cgtgcagctg
    18241 tgggcggaaa gtctcggcaa ggacggccaa ggcaccacgc cgattgcttg tctggggccc
    18301 gtcgaccaac acagccagtt gcagctgttc atggctggcc cgcgggaaca tctgatcacg
    18361 gtggtgcgcg cgccgacggc gagccaggga ccgcgactgt ccacagatct cgcaggtgtg
    18421 gccggcttgg agtatctcgg cggcaagacg gtcggcgatt tggttgctgc gcaggccatc
    18481 gccgtgcccg aagcgctggg gcgcgccggg cggcctgtac gcacattcga cctcgcacgg
    18541 cttgacgaag ccaatcttgg cgccttgatg atgcatttaa tgctggaaac gattctcgcc
    18601 gggcgcctgc tcggcgtcga tccgtttgat cagcctgccg tcgagctggc caaggtgatt
    18661 acgcgcgaac gcgttgccgc attaaaggcc tgatgggcgg ctgcatgggg aggaggcagt
    18721 cccagccgtg cccatccgtc agctcagtgc cgagacgatc aatcgcatcg ccgccggcga
    18781 ggtgatcgaa cggcccgcaa gcgtcgtcaa ggagctggtc gagaacgcca tcgatgctga
    18841 ggccaccgcc gtcgagatcg tcaccgtcgc cggcggacta tccctcatcc gcgtgagcga
    18901 taacggtgtg ggcatgaccg gctcggatct gctgctggcc gtggatcggc acgccacctc
    18961 aaagctcgac gaggaagacc tcttcaatat cgcaacgctg ggcttccgcg gcgaggccct
    19021 gccgtcgatt ggcgctattg cccacctggc gctggcatcg cgttcgcggg cggccaacga
    19081 tgcgcacgag atcgtggtgg atcgcggcaa caaggcagcg ctgcgcccag cagcaattgc
    19141 ccgcggcacg cgcgttgagg tgcgcgagct gttctcggcc acccctgcca ggctcaagtt
    19201 cctcaaaagc gagcgggcgg aaaattttgc catcaacgag gtcgtcaaga agatcgccct
    19261 ggcgcagccg ggcatcgcct tcagcctgac gaccggcgag cggaccgccc ttagactgcc
    19321 gcaggaagcg gcgggacccg cgggacatct gtcgcgccta ggccgcatca tgggccagga
    19381 tttcctgcgc gatgcgctgc ccgtggaggc cgagcgcgag ggcattcgcg tcacaggctt
    19441 tgccggcctg cccacgctca atcgccctga cagcggccaa cagcacctgt tcgtcaacgg
    19501 ccgaccggtc agagaccgct tgctgatagg cgccgtgcgg gccgcctacg ccgatctgat
    19561 cccgcgcggc cgctcgccgc tgcttgcgct gtttgtgacg ctgccggcgc gcgaggtcga
    19621 cgtcaatgtg catccgacca aggccgaggt ccgtttccgc aacgtgcacg gcgtgcgcag
    19681 cctggtgctg ggtgggctcc gcgccgcttt ggaagctgcg ggacatcgcg cctccgacag
    19741 gggcggcacg gccatgctcg ccgccctggc gcagtccgca ggtgacggca aagccgcccc
    19801 cggcgtgaat tttccggtcg ccccggcggc accagcctgg agcggcagag cctatccgcg
    19861 ctcctcgacg gccacgcttg cagccatgca agcgccgctc gacggtatgg ccgcgccgag
    19921 cgccgattgg agcgccgctg cggcgccggc agcgcccgaa ctcgtcgatc gccccctggg
    19981 ggcggcgcgc gctcagttgc acgagaccta tattgtcgcg caaacgcgtg atgccgtgat
    20041 tatcgtcgac cagcacgccg cccacgagcg gctcatctac gagcgcatca aggcccagct
    20101 tgccgacggc ggcgtggccc gacaggcgct gctgatccct gtgatcatcg aattggatgc
    20161 ggacgagatc gcggggctcg ccgaagcagg cgccgcgttg ctcgaactcg gtctgcggct
    20221 cgagaccttc gggccgggcg ccgtgatggt gcgcgagatc ccggcgctgc tcggggatgc
    20281 ggacatcggc ggcctcgtca aggatctggc gcgggaggcc ctgcgggaaa gcgccggcgc
    20341 cttgcttaac gaacgcctgg cagccgtatg ctccagcatg gcctgccatg gcagcgtgcg
    20401 cgccgggcgt cgcctcacgg ggcaggagat gaacgcgtta ttgcgcgaaa tggagctgac
    20461 gccccatgcc ggccaatgca atcacggccg acccacctac gttgaactca agcttgccga
    20521 tatcgagcgc ctgttcggcc ggcgttagcc cgccaacgcc aaccgcgcgc ttgcgggggt
    20581 gccttgctgg cgcggccgtt gccagcttgc caagcggcgc aaaaggcgct cccctggcgg
    20641 ccctggcacg cggtgcttcc atcgggagct atctcgctct atgagtgcgc gcgaagccca
    20701 caataggggt gaggatgctt gcgtggtcgg cggtttggcc gacgccgcga gcgggctcga
    20761 gcgggtgcaa tgtcgtttgt ggtgatgaag tttggtggca cttcggtagc caatttggag
    20821 cgcatccgca atgtggcgcg ccatgtcaag cgcgaggtgg atgcgggcaa ccacgtggcc
    20881 gtggtggtct cggccatggc cggggtgacc aatcagctcg tggcctgggt gcgggaggcc
    20941 tcgccgctgc acgacgcgcg cgaatatgac gcggtcgttg ccaccggcga acaggtgacg
    21001 gcaggtctgc tggccatcgt gctgcagtcg atggcaattc cagcgcgctc ctggcagggc
    21061 tggcaaatcc cgatcatgac ggacgacgcg catggcgttg cccgcatcaa ggatattccc
    21121 ggcgacaaga tccgcgcccg cctggaacag ggcgaggtcg ccgtcattac cggcttccag
    21181 ggcatcgagc ccaatcgcaa tcgcatctcg acgctgggac gcggcggctc cgataccagc
    21241 gcggtggcgc tggccgtggc ggtgcgggcc gatctttgcg acatctacac cgatgtcgat
    21301 ggcgtcttca ccaccgatcc gcgcattgtg ccgagggctc ggcggctggc cagggtctcc
    21361 tacgaggaga tgctggaaat ggcctccctg ggctccaagg ttttgcagac gagatcggtc
    21421 gaactcgcca tggtgcaccg gatgcccttg cgggtgttgt cgagcttcat gccccccgaa
    21481 aacttgacgc cagtcgacgc gcaatcctgg gacgagatcg gcaccattat ttgcagtgag
    21541 gatgagatcg tggagcagca cgtcgtcagc ggcatcgcct atgccaagga tgaggccaag
    21601 gtttcgctga tccgggtcga ggacaagccc ggggtcgccg cgcgcgtttt tgggccgctg
    21661 gcc
//
LOCUS       DASTMT010000306         4206 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_501877, whole genome shotgun sequence.
ACCESSION   DASTMT010000306 DASTMT010000000
VERSION     DASTMT010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4206)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4206)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4206
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_501877"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1029
                     /locus_tag="VFR00_07150"
     CDS             <1..1029
                     /locus_tag="VFR00_07150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008968745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HEU0159071.1"
                     /translation="PWQMEHKTATEYGRVAAEAAKMMRWVDPSLVLAACGSTSRNMPT
                     FGRWDDEVLEHTFELVDFLSLHTYLNNYRGDSAAFLAAPDLMDFFIEEVIAIADAVAA
                     RRRSPKRLLLSFDEWNVWYRTRRTRQDRVKEGWPVAPPILEEIYTMEDALAFGGACIS
                     LLNHADRVKCACLAQLVNVIAPIMTETGGGAWRQTIFYPFQHMSRHGRGRVLKCQVHA
                     EAYAAAYYDPRGSLDQVFPIPAVPYLKLAAVATAGGGLSLFALNRDLGQPMTVSVEAR
                     GFADLGVAEALELRHPDLSAVNSRAAPDRVAPQPLERVECDGGKVTLALAPASWNLIS
                     LAPRANGA"
     gene            1091..2218
                     /locus_tag="VFR00_07155"
     CDS             1091..2218
                     /locus_tag="VFR00_07155"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEU0159072.1"
                     /translation="MPTLADTPLSGVVPIAPTPFRDNGDLDLESQRRVLDCMIDQGVD
                     GICILANYSEQFLLSDEERNVLLDLALGHVKARVPVVVTCSHFSTRIAAERARRAATA
                     GAQMLMLMPPYHGALLRADEAGTFDHFARIAEAAGGLPIMVQDAPLSGVNLSVPFLAR
                     LARELPQVAYFKIEVPGTAAKLRALIAAGGPAIRGPFDGEEGITLLADLDAGATGTMP
                     SALLPDLIKPVLEHHRAGRRAEAVAAYARVLPLINYENRQCGLRATKTVMALGGVIAS
                     EAVRHPLEPLPAATRAGLIELVGALAPLAARWGKSIVWGCLGQQKGVYHDKRARRLSI
                     ACGPRCLRDGRRQRHWCGHRCRLGATKGKSCLCRYRRKSLR"
     gene            2115..2831
                     /locus_tag="VFR00_07160"
     CDS             2115..2831
                     /locus_tag="VFR00_07160"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015666821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU0159073.1"
                     /translation="MTGGGSGIGAAIVAALAQQKAKVAFVDIDEKASGEVQARLEALG
                     AVSCFERCDIRDVAALRRAIQNARERLGPITILINNAARDDRHASEEVTPEYWDERMA
                     VNLKHQFFAAQAVLPDMKAAHGGSIINFGSLSWMTGQGGMVAYTAAKSAVLGLTRSLA
                     RDFGPDNIRVNAIAPGWILTERQRALWLTPQSHARLMEAQCLKRELNGDDIARVVLFL
                     CSDQAGAITSQQIVVDGGRL"
     gene            complement(2877..3638)
                     /locus_tag="VFR00_07165"
     CDS             complement(2877..3638)
                     /locus_tag="VFR00_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016846279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0159074.1"
                     /translation="MAELDVEDLSLAFGGLQVLEKVSLRVGPAELVALIGPNGAGKTS
                     VLNCISGLYHGSGRIRLRGRDLAGLPPRVIAGLGIARTFQHGELFQHLSVLDNIMVGR
                     HTRVATRPLGEALFLPSVRRQEIAQRDAVERIIEFVELERVRYADAGALPFGTQKLVG
                     LARALALEPAVLLLDEPSAGLSRDERDDLARFLLRIKHELALPMLWIEHDMQMVADLA
                     DRIHVLNYGRTLAAGPPSQVLNDPAVIAAYLGVRS"
     gene            complement(3631..>4206)
                     /locus_tag="VFR00_07170"
     CDS             complement(3631..>4206)
                     /locus_tag="VFR00_07170"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU0159075.1"
                     /translation="AQPHVTTAMGIVLVPERGKVFPNLTVTENLLVPVAARTARAEQR
                     RREALALEVFPRLAPIRQRTAGLLSGGERQMLAIAAALVCQPQLLLVDELSLGLAPVV
                     VEEILQGLLAIRKALALTLLIVEQNAALTLDVIDYGYVLENGRIVLDGSAQRLRSHPD
                     VREFYLGQSAAGQRRSYRDVKQYRRNRRWYG"
BASE COUNT          704 a         1413 c         1394 g          695 t
ORIGIN      
        1 ccttggcaaa tggagcacaa gacggcgacc gaatacggcc gtgtcgccgc cgaggccgcc
       61 aagatgatgc gctgggtcga cccgagcctg gtgctggccg cctgcggctc gacctcgcgc
      121 aacatgccga ccttcggccg ctgggacgat gaggtgctcg agcacacctt cgagctcgtc
      181 gatttcctgt cgctccacac ctatctcaac aactatcggg gcgacagtgc ggccttcctg
      241 gcggcccccg atctgatgga cttctttatc gaagaggtca ttgccatcgc cgatgcggtg
      301 gcggcgcggc gtcgctcgcc caagcggctg ctcttgagct tcgatgaatg gaacgtctgg
      361 taccgcacgc ggcgcacccg ccaggatcgc gtcaaagagg gctggccagt ggcgccgccc
      421 atcctcgagg aaatctacac catggaggat gcgctcgcct tcggcggtgc ttgcatttcg
      481 ctcctcaatc acgccgatcg ggtcaagtgc gcctgtctcg cccagctcgt caacgtgatc
      541 gccccgatca tgaccgagac gggcggcggc gcctggcggc agaccatctt ttatcccttc
      601 cagcacatga gccggcatgg ccggggacgc gtgctcaaat gccaggttca tgcggaggcc
      661 tacgctgctg cctactatga tccgcgcggg agcctcgacc aagtgttccc cattcccgcc
      721 gtgccctatc tcaagctggc ggcggtcgcc accgccggtg gtgggctcag cctgttcgcg
      781 ctgaaccgcg acctggggca gccaatgacc gtgagcgtcg aggcgcgcgg cttcgccgat
      841 ctcggggttg ccgaggcgct cgagctccgc caccctgacc tgagcgcggt caacagtcgg
      901 gcggcccccg accgtgtcgc gccgcagccg ctcgagcgtg tcgaatgcga tggcggcaag
      961 gtgacgcttg ccttggcgcc ggcgtcctgg aacctgatca gcttggcgcc cagggcgaat
     1021 ggcgcctgag gcttcggcca taccgggtag ttagagacaa cgagaaacac cttctgcgag
     1081 aggagctgcc atgccgaccc tagccgacac accgctcagt ggcgtggtgc cgatcgcgcc
     1141 aacacccttt cgagacaatg gcgatctcga ccttgagagc cagcgccgtg tgctcgattg
     1201 catgatcgac cagggggtcg atggcatctg cattctcgcc aactactccg agcagttcct
     1261 gctctccgac gaggagcgca acgtcctgct cgaccttgcc ttgggccacg tcaaggcgcg
     1321 cgttcccgtc gtcgtcacct gcagtcattt ttccaccagg attgccgccg aacgcgcgcg
     1381 ccgcgcggcg accgccggcg cccaaatgct gatgctcatg ccgccttacc acggagcgct
     1441 gttgcgcgcc gacgaggcgg gcaccttcga ccactttgcc aggatcgccg aggcggccgg
     1501 cgggcttccc atcatggtgc aggatgctcc cttgagcggc gttaacctga gcgttccgtt
     1561 cctggcgcgg ctcgcccgcg agttgccgca agtggcctat ttcaagatcg aagtacccgg
     1621 cacggcggca aagttgcggg cgctgatcgc cgccggtggt cctgccatcc gcggcccctt
     1681 cgacggcgag gaaggcatca cgctgctggc cgacctcgat gccggcgcga cggggacgat
     1741 gccgagcgcg ctgctgcccg acctcatcaa gcccgtcctc gagcaccatc gcgccgggcg
     1801 gcgcgccgag gccgttgccg cctacgcccg cgtgctgccg cttatcaact acgaaaaccg
     1861 ccaatgcggc ttgcgcgcga ccaagacggt gatggcgctg ggcggcgtga tcgccagcga
     1921 ggccgtgcgc catccgctcg agccgctgcc agccgccacc cgggcgggtc tcatcgagct
     1981 cgtcggcgcg ctggcgccgc tggcggcacg ctggggcaaa tcaatcgttt ggggctgcct
     2041 tggccaacaa aagggggtct accatgacaa gcgcgcacgc cgtttatcca tcgcttgcgg
     2101 gccgcgttgc cttcgtgacg ggcggcggca gcggcattgg tgcggccatc gttgccgcct
     2161 tggcgcaaca aaaggcaaaa gttgcctttg tcgatatcga cgaaaaagcc tcaggtgagg
     2221 tgcaggccag gctcgaggca ctgggcgccg tcagctgctt cgagcgctgc gacatccgcg
     2281 acgtggcggc gctgcggcgc gccatccaaa acgcccgcga gcggttgggg ccgatcacca
     2341 ttctcatcaa caacgccgcc cgcgatgacc ggcacgccag cgaagaggtg acgcccgagt
     2401 attgggatga gcgcatggcg gtcaatctca agcaccagtt ctttgccgca caggccgtgt
     2461 tgcccgacat gaaggcggcg cacgggggat cgatcatcaa tttcggctcg ttatcctgga
     2521 tgacgggcca gggtggcatg gtcgcctata cggcggccaa gtcggccgta ctggggctca
     2581 cccgctcgct ggcgcgcgac ttcggccccg acaatatccg ggtcaatgcc atcgcgccgg
     2641 gctggatcct gaccgagcga cagcgtgccc tgtggctgac gccgcagagc cacgcccggc
     2701 tgatggaggc gcagtgcttg aagcgcgagc tcaatggcga cgacattgcc cgcgtcgtgc
     2761 tgtttctgtg ctccgaccaa gccggcgcca ttaccagcca gcagattgtc gtcgacggcg
     2821 ggcgcctcta gcggagcgcc tgcagccgaa atgcggtaga ccggcactgc tccagcctag
     2881 gagcgcacgc ccaggtaggc ggcgatgaca gctgggtcat ttaggacctg gctgggaggc
     2941 cccgcggcca gcgtgcgccc gtaattgagg acgtggatgc ggtcggcaag atcggcgacc
     3001 atttgcatgt catgctcgat ccagagcatc ggcagggcca attcgtgctt gatgcgcagg
     3061 agaaagcggg cgagatcgtc gcgttcgtcg cgcgagagcc cggccgaggg ctcatcgagc
     3121 aagagcacgg cgggctccag cgccagcgcc cgtgccaacc ccacgagctt ctgcgtgccg
     3181 aacggcagcg cccccgcgtc ggcgtagcgc acgcgctcga gctcgacgaa ctcgataatg
     3241 cgttccacgg cgtcgcgttg cgcgatttcc tgccggcgca cgctgggtag aaacagggcc
     3301 tcgccgaggg gccgggtggc aaccctggta tggcgtccga ccatgatgtt gtcgagcacg
     3361 ctcaggtgtt gaaagagctc gccgtgctgg aaggtgcggg cgatgccaag cccggcaatg
     3421 acccggggcg gcaaccccgc aaggtcgcga ccgcgcaggc gaatgcgacc gctgccgtga
     3481 tagagcccac taatgcaatt gagaacgctg gttttgcccg cgccgttggg accgatcagc
     3541 gccaccagct cggccgggcc gacgcggagc gagaccttct ccagcacctg gagcccgccg
     3601 aaggcgaggc tcaagtcctc gacgtcgagc tcagccatac catctccgat tgcgccggta
     3661 ttgcttgacg tcgcgatagc tgcgccgctg cccggccgcg gattgtccga gatagaattc
     3721 gcgcacgtcg ggatgcgacc tgagccgttg ggcgctgccg tcgagcacga tccggccgtt
     3781 ctccaggaca tagccgtagt cgatcacgtc caaggtcagc gccgcgttct gctccacgat
     3841 caggagcgtg agcgccaggg ctttgcggat ggccagcaag ccctgcaaaa tctcctcgac
     3901 gaccacgggg gcgagaccca gcgacagttc gtcgaccagc aacagttggg gttggcagac
     3961 gagggcggca gcgatcgcca gcatttgccg ctcgccgccc gacagtaagc ccgccgtgcg
     4021 ctgccggatc ggcgccaggc gtgggaagac ctccaaggcg agcgcctcgc gccggcgctg
     4081 ctcggcgcgc gcggtccgcg ccgccacggg caccaacagg ttctcggtca cggtcagatt
     4141 gggaaaaacc ttgccgcgtt ccggcaccag gacaatgccc atggcggtgg tgacgtgggg
     4201 ctgggc
//
LOCUS       DASTMT010000307         3683 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_2465080, whole genome shotgun sequence.
ACCESSION   DASTMT010000307 DASTMT010000000
VERSION     DASTMT010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3683)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3683)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3683
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2465080"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1095
                     /locus_tag="VFR00_07175"
     CDS             <1..1095
                     /locus_tag="VFR00_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011085735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEU0159076.1"
                     /translation="RHGSSEAPPLTMFYTSGTTGAPKGVRRGAPSAAQMAVSDRLRRE
                     VFGIVPGIRSAIPGPLYHSAPNAFAMRAGRIGEVMVLMPRFDPLALLELIERQRIDTI
                     FMVPTMFVRLIKLPEAVRRRFDVSSLRFVIHAAAPCPTDVKQAMIDWWGPVIHEFYGS
                     TESSAVTFASSADTLAKPGTVGRAVAGAQLRILDAAGTPLPAGEVGEIFTRFAELPDF
                     TYHNKPDERAAIERDGFITSGDVGYLDADGYLFLCDRKRDMVISGGVNIYPAEIEAQL
                     LRIAGVKDCAVFGIPDAEFGEKLMAVVELMEGRSLTPASLQAELARHLAGYKVPRIIE
                     IGRDLPREDSGKIFKRRLREPYWQAVGRNI"
     gene            complement(1110..1328)
                     /locus_tag="VFR00_07180"
     CDS             complement(1110..1328)
                     /locus_tag="VFR00_07180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0159077.1"
                     /translation="MSAPRFYRYPTMYSAEAPPLLPPGAMSFAPFAQQPYVLVSQGIW
                     YWPGANDFKMPQVPGFEPVIDPSRVISW"
     gene            complement(1740..2024)
                     /locus_tag="VFR00_07185"
     CDS             complement(1740..2024)
                     /locus_tag="VFR00_07185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0159078.1"
                     /translation="MPTLDRTVIAALTMLLVGSVLGWFANLAVHGATFDRAADLIAGM
                     MGGVVLGGLVNYCLPGSREASTVRWVAAAAVGAALAILWTRYLTLSFWAP"
     gene            complement(2209..2373)
                     /locus_tag="VFR00_07190"
     CDS             complement(2209..2373)
                     /locus_tag="VFR00_07190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0159079.1"
                     /translation="MHRTSETTRRLGQALRQEMPLCQPLPPELQALLLRLALAEAERR
                     HYGASGRSAA"
     gene            complement(2564..>3683)
                     /gene="argE"
                     /locus_tag="VFR00_07195"
     CDS             complement(2564..>3683)
                     /gene="argE"
                     /locus_tag="VFR00_07195"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015596254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008777 - acetylornithine deacetylase
                     activity [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HEU0159080.1"
                     /translation="LVAFDTESAKSNIALIRFVEDYLTQHGIASARVPTPDGEKASLF
                     ATIGPSDRPGLALSGHTDVVPVAGQRWDSDPFTLTERDGRLYGRGSCDMKGYLACALA
                     MVPQFKSRRLNMPIHLAFSYDEEVGCTGVRPMIAELGKRLPLPRMVFVGEPTEMGVVD
                     AHKGPVRWRVELTGRAAHSSMPHYGVNAIAYAGRLLGELLRMEEDLKAGAQDPRFDPP
                     WTTLQITQIEGGTASNVVPVPCWFGWEIRALPGFDPMLLDRRLRTFAETHCLPEMRRV
                     APETQIRIAATNQVAAYAADAASGIVPLTLQLAGQNETYAVSYCTEAGLFQGGGAPAI
                     VCGPGNIAQAHTANEFVSIAELEKCLRFLARLADWAAA"
BASE COUNT          614 a         1243 c         1216 g          610 t
ORIGIN      
        1 cgtcacggca gcagcgaggc gccgccattg accatgttct acacatccgg caccacgggc
       61 gcgccgaaag gcgtgcgacg aggagcgccc agcgccgcgc agatggccgt ctcggatcgc
      121 ttgcggcgcg aggtgtttgg catcgtgccg ggcatccgct ccgctattcc cggcccgctc
      181 tatcactccg ctcccaacgc cttcgccatg cgcgcgggtc gcattggcga ggtgatggtg
      241 ctgatgccgc gcttcgatcc cctcgcgcta ttggagctga tcgaacgcca gcgcatcgac
      301 accatattca tggtgccgac catgtttgtg cgcctcataa aattgcccga ggcggttcga
      361 cgtcgctttg acgtctcctc gctacgcttc gtcattcacg ccgcggcgcc ctgcccgacc
      421 gacgtgaaac aggccatgat cgactggtgg ggtccggtga tccacgaatt ttacggctca
      481 accgaatcga gcgccgtgac ttttgccagc agcgccgaca cgctggccaa gcctggtacg
      541 gtcggccgcg ccgtcgcggg cgcgcaattg cgcattctcg atgcagcggg caccccgttg
      601 cctgccggag aggttgggga gatcttcacg cgctttgccg agttgccgga cttcacctat
      661 cacaacaagc ccgacgagcg cgcggccatc gagcgcgacg gctttattac gtcgggcgat
      721 gtgggctatc tcgacgccga cggctatctg tttctgtgcg accgcaagcg cgacatggtg
      781 atttccggcg gcgttaacat ctaccccgcc gaaatcgagg cccagctgtt gcgcatcgcc
      841 ggcgtcaagg attgcgccgt gttcggcatt ccggacgccg aattcggcga gaagctgatg
      901 gcggtggtgg agcttatgga gggccgcagt ttgacgcccg cgagcctgca ggccgaactt
      961 gcccgccacc tcgccggtta caaggtgccg cgcatcatcg aaatcggacg cgatctgccg
     1021 cgggaggact cgggcaagat tttcaagcgc cgcttgcgcg agccctactg gcaggcggtt
     1081 gggcgcaaca tttgacgcgc cgccaacccc taccaactaa tcacgcgact ggggtcaatg
     1141 accggttcga agcctggcac ctgcggcatc ttgaaatcgt tggcgcccgg ccagtaccaa
     1201 atgccttggg acaccagcac gtagggttgc tgcgcgaagg gcgcaaaact catggcgccg
     1261 ggcggcagca gcggcggggc ctcggcgctg tacatcgtcg gatagcgata aaaacgcggc
     1321 gcgctcacgc ggttcactgt cgctttcggg cgcgctacgt ttaggcccgc gttgcgtgac
     1381 ttgcgggacc ccgccgtcgg cttgggccgc ggcgcgcgga ccgcctcggc caccgcaccg
     1441 cttacgcccg gacacggcgt ctcgggccag cagacctgtc ccgggtgata gacctgcgcg
     1501 gccgcgggcg ttgtcatcgc cgcggcgacc gccagcagcg cggcccgccc cgccccgcct
     1561 cgtcgcattg cctcacctca ttcgtgcgcg cctggacccc tgaccgctgg tcccgccaac
     1621 tttgccgtgt gcccggcggg tcgccgcggc cgctcgcgtt ctacgatccc gtgccgcgcc
     1681 ctgtcgtgcg tcagccgcgg agatggttgg caaagacggt taaggttgcc ggcggcggct
     1741 cacggcgccc aaaaggacaa ggttagatag cgcgtccaca ggatggccag ggccgcgccc
     1801 accgccgcgg cggccaccca gcgcacggtc gaggcctcgc ggctgccggg caggcagtag
     1861 ttgacgagcc ccccgagcac cacgcccccc atcatgccgg cgatcaaatc ggcagcacgg
     1921 tcaaaggtag ccccgtgcac ggccaggttc gcaaaccagc cgagcaccga ccccacgagg
     1981 agcatcgtca gcgccgcaat gacggtgcga tccaaggtag gcattctccc ccactcgcaa
     2041 atgcttggca tttgcaagac tatactgcgc ccggcgccgg ggcgacaagc ggccgatcgg
     2101 gctccggctt ggcctgggtg cgtgcgggcc tcgatgggcc aaggccttgc cgagcccgct
     2161 tgccaccttt ggcggagacg cgaggtcgcg gggagaggac gtcggccctc aggcggcgct
     2221 gcgcccggag gcgccgtagt ggcggcgctc cgcctcggcc aacgccagcc gcagcaagag
     2281 agcttgcagc tcgggcggca acggctggca gagcggcatc tcctggcgca gagcctgccc
     2341 gagtcgccgg gtggtttcgc tggtgcggtg catggtggtc cctcttcacg tgcgccggca
     2401 aaacgccgcg gcgtcgcctt cgttccccga tcgaggcaaa ttcatgcttt caaatcaaag
     2461 agttaacaaa aggtaaatcg gttacaggga atcgcctggc ggcacacgcg aatgacggac
     2521 cgcagacgca agggcgcgcg ccccccgaac gctgccggca aggtcaggct gccgcccagt
     2581 cggcaagacg ggccaaaaag cgcaggcatt tttccagttc ggcgatgctc acaaactcgt
     2641 tggcggtgtg ggcttgggca atattcccgg gcccgcagac gatggccggc gcaccgcccc
     2701 cctggaacag gcccgcctcc gtgcaatagg acaccgcgta agtctcgttc tgcccagcca
     2761 gttgcagggt cagcggcacg atgccggagg cggcatcggc cgcataggcc gcgacctggt
     2821 tggtggcggc aatgcggatc tgggtctcgg gtgcgacgcg acgcatttcc ggcaggcagt
     2881 gcgtctcggc aaacgtacgc aagcgccgat cgagcagcat gggatcgaag cccggcagcg
     2941 cgcggatttc ccagccgaac cagcatggca ccggcaccac gttggaggcg gtgccacctt
     3001 cgatctgcgt gatctgcaac gtggtccaag gcggatcgaa gcgcggatcc tgtgccccgg
     3061 ctttcaggtc ttcttccatg cgcaacagtt cgcccaacag gcggcctgca taggcgatgg
     3121 cgttgacgcc atagtgcggc atgctcgaat gcgctgcgcg tcccgtcagc tcgacgcgcc
     3181 agcgcaccgg gcccttgtgc gcgtcgacga cgcccatttc ggtcggctcg cccacgaaca
     3241 ccatgcgcgg cagcggcagg cgtttgccaa gctcggcaat catcggccgc acacccgtgc
     3301 agcccacttc ctcatcgtaa gagaaggcaa ggtggatcgg catgttcagg cggcggctct
     3361 tgaactgcgg caccattgcc agcgcgcagg cgaggtatcc cttcatgtcg cagctgccgc
     3421 gaccgtagag cctgccgtcg cgctcggtca gcgtaaaagg atcgctgtcc cagcgctggc
     3481 cggcaacggg gacgacgtcg gtgtgacccg acagcgccag gcccggccga tcgctgggcc
     3541 cgatcgtggc aaacaggctt gccttctcgc cgtcgggcgt gggcacgcgg gcgctggcga
     3601 tgccgtgctg ggtcagatag tcctcgacga agcggatcag ggcaatgttg ctcttggcac
     3661 tttcggtgtc aaaggcgacg agg
//
LOCUS       DASTMT010000308         8792 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_3696221, whole genome shotgun sequence.
ACCESSION   DASTMT010000308 DASTMT010000000
VERSION     DASTMT010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 8792)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8792)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8792
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3696221"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..792
                     /locus_tag="VFR00_07200"
     CDS             <1..792
                     /locus_tag="VFR00_07200"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HEU0159081.1"
                     /translation="VEEILRFFDHYLMGLPTGLEREAPVHYFALGREAWRAAADWPPA
                     AQTQTLYLAAPGALSAAPQAPAHDSHKVSFAWGSGHMTRYERIAAIDTPQYYADWHGR
                     DAALLVYTSEPLPEPLVLAGHAVVSLWLEADQPDAAIHVYLEDIGADGRCRYVTEGAL
                     RALHRQTAPCPAHHRTSWPWRTFARADAALLPRGEVVELTFALLPVAWAFAAGSRLRL
                     AISGADCDHVVQVPHGRPPLLRIHHGSRHPSRLALPCLSGGASAL"
     gene            840..1613
                     /locus_tag="VFR00_07205"
     CDS             840..1613
                     /locus_tag="VFR00_07205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003508915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0159082.1"
                     /translation="MALLQVIGVEHAFYGVEALSGVDLAVEPHTVTGLIGPNGAGKTT
                     LFNCITGIVPPDRGRILFDGADIAGLRPDQVSRRGLARTFQIARGLPRLSVIENLMLY
                     GPRQPGERLLPALLRPASVRRREEALVARAVAIARRLNLFSVIANPASDLSGGQKKLL
                     EIGRVLMSEPKLILLDEPVAGVNPTLAREIGAHLRALASEGLTILLIEHHLDMVARLC
                     DHVIVLAEGRRLAEGSFAAVAADPAVQEAYMGRRRAGVP"
     gene            1628..2323
                     /locus_tag="VFR00_07210"
     CDS             1628..2323
                     /locus_tag="VFR00_07210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019567204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0159083.1"
                     /translation="MANGVVAGYGAQDEILKGVDLVVDAGEVVCIIGPNGAGKSTLLK
                     AIAGLLRPKLGSVTLRGRSIGGLPAREISRLGLAYVPQEFNVFPTLTVRENLEIGGYI
                     DRKAAARRIEGVLARFPVLADKARHAARTLSGGERQMLAMAMALMVEPVVLLLDEPSA
                     GLSPRAAERLFDAVVEIHKDGVAVAIVEQNANEALAIADRAYILVDGKNSRTGEARAL
                     AADPEIRRLFLGA"
     gene            2368..3567
                     /locus_tag="VFR00_07215"
     CDS             2368..3567
                     /locus_tag="VFR00_07215"
                     /inference="COORDINATES: protein motif:HMM:NF013275.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0159084.1"
                     /translation="MPGPTRRALITGAAAASAIAASPFPSVAQSDPIKLGVLTPLTGA
                     GGQDGPRMLKAMEAVIAEVNKAGGVLGRKLETVVEDDQTNPEAAVRAARKLIEVNKVP
                     VIMGTWASAVTSAVAPVCWEAKTFLCTVSGADSITQLPHQGYLIRTQPNSKLQATAHA
                     DFIAGLGKKKVAVIGVQAPFALSTKEHLDAVLKTKGAKVVAHIIYEKDKATYRSEIDQ
                     AMRSDPDFLYLNGYAPDTVVVLRDLYKANINLPKFCQSYAVPQKTLDTIPAEVSAGTY
                     TGQPSADIEGKAFKLAAARLGIAEPDSYEAQATDWASLVILTLAKAKEVTGTALKENV
                     RKISQGSGEKVWSAVEGLQALAAGKEINYEGASGPCDFTEIGDIEGCRFRYMEVVDGK
                     LRFLKVV"
     gene            3614..4474
                     /locus_tag="VFR00_07220"
     CDS             3614..4474
                     /locus_tag="VFR00_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019567206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEU0159085.1"
                     /translation="MEVWQLILNGLMSGTILAVPAIGLSAIFAVLRFTNFALASHMTI
                     GAFAGFVANVWLAWPVVPSLLAAFLIAGLVGVLSDEFILKPFRAAGFITTAIGSIALT
                     IALENGVRFVFGNELRGYDLPIRRDWVLGGLRVSPQQLENLLIALAAMGLLFAFLFFT
                     RTGRAMRAFADNPMLASIKGINADMVARLVSFVAMGLAGLGGMLIGLDTTIDPLVGFK
                     SILTIFAAAVVGGLGSLPGAVVGALLVGVGEELSLLILQPAYRSAIGFLAILMVLTLR
                     PRGILGARAY"
     gene            4484..5419
                     /locus_tag="VFR00_07225"
     CDS             4484..5419
                     /locus_tag="VFR00_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009449270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEU0159086.1"
                     /translation="MEHYLVAIGVTAGVYALMALGMNVIWGMAGLINLGLVGFFAVGA
                     YVSGLLTLKLGVPIVIGVAAGTVMAALVGVLVALITVNLRGDYLAIVTLGFAESLRIV
                     MSNELWIANGTDGLSGIPGPYRAELGPHLFNLFYLAIVVGAVIVLFFLVDRLVNSPFG
                     RVLRAIRDDDVIAEVAGKHVAVFKIKAFAFGSAALGLAGALYGHFTSYIAPDLFAPLL
                     TLYVKLSLLTGGLGNSKGAVLGAVLVVFFLESTRFLAPLIPGLSAVQAAAVRELAISV
                     ALLVILRFYARGLLPERIEPPALAPEAAAPALNRI"
     gene            5442..5945
                     /locus_tag="VFR00_07230"
     CDS             5442..5945
                     /locus_tag="VFR00_07230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEU0159087.1"
                     /translation="MRKTKRGLEMADVAAILERSSEAGDVREVYREIERISRECVGWR
                     LLTILRYVEADAAVERLYSSDEKAYPVGGRKPLDKIATSHAAMEKGDVFLAATREEVR
                     TAFFDHELIFSLGITAILNAPIRQGGRRLGTLNFCGEEGAYGTAEVASARLLAGLVAP
                     SLLRGGD"
     gene            5999..7234
                     /locus_tag="VFR00_07235"
     CDS             5999..7234
                     /locus_tag="VFR00_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018322192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU0159088.1"
                     /translation="MTQYHFKNFELLDPEREELVGGHELLVEGALIRELSAKPIKAPE
                     ANVIDCGQRTLMPGLIDSHVHVVLSEVSLPRLEHVPLTLMTARAAVLMRNMLDRGFTS
                     VRDTGGADWGLKEAVDKWLLPGPRLFIAGKALGPTGGHSDGRRRTDHGTRCHCCDALH
                     FAMGLADGVAEVRKAVREEMRQGCDQVKIMMSGGVASPYDPLDSLQFSPEEVAAAVEE
                     AHAFGRYVCAHAYSAEAITRAAHAGVRTIEHGNLIDEASAKLMASKGMYLIANLVTYY
                     EMKKRAAEYGMSADMLAKNDLVIDGGLRSLEICKRAGVPVAYGTDLLGQLQSEQSREF
                     MIRAEVQKPIEVIRAATLIGAEIVRQVGKLGCLKPGAFADLLLVDGNPLLNLGVLQEQ
                     GKFLAAIMKGGRFHKNRLH"
     gene            7238..8473
                     /locus_tag="VFR00_07240"
     CDS             7238..8473
                     /locus_tag="VFR00_07240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018647167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU0159089.1"
                     /translation="MAQLHFKNFRLLEPAYGELRGGYELLIEGERVRELSERPIVSRA
                     AEVVDCGGRTLMPGLIDCHVHVIHSEVNIRFLEAMPLTLLTARASLRLRAMLDRGFTT
                     VRDTGGADWGIKTALEQGYLVGPRLYIAGMSIGPTGGHSDGRRRTDDSDGCSCCNGLK
                     FKSAVADGNDEVRKAAREQMRQGADHVKIMMSGGVASPYDPLDSLQFSEGEVAAVVEE
                     AHAFGRYVCAHAYSAQAITRAARLGVRTIEHGNLIDDAAARLMASKGMFLVANLVAYY
                     AMKERAQKFGMSAESLAKNEIVLEGGLKSLEICKRHGVPVAYGSDLLGELHWDQSREF
                     SLRREVVSPIEIIRSATTIAAQVLRLEGKVGTLLPGAWADLLLVDGDPLKDLAVLEDQ
                     GRHLSIIMQAGRFHKRRLH"
     gene            8529..>8792
                     /locus_tag="VFR00_07245"
     CDS             8529..>8792
                     /locus_tag="VFR00_07245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0159090.1"
                     /translation="MRPGMANARSLGRLALKAGAGVTLLYILTPLLFVTWLAFFKQEI
                     PSFPPEGYSLRWFLAIPGNDRFVSGFLLSLEVAVIATAIGLLLG"
BASE COUNT         1373 a         2840 c         2957 g         1622 t
ORIGIN      
        1 gtcgaggaaa tcctgcgctt tttcgatcac tacctgatgg gattgccgac cggactagag
       61 cgcgaggcgc cggtgcacta cttcgcgctg ggtcgggagg cctggcgggc ggccgcggac
      121 tggccgcccg cagctcagac gcaaacgctc tatctcgccg ccccgggggc gttgagcgcg
      181 gccccgcagg ccccagcgca cgacagccat aaggtctcct tcgcctgggg gagcgggcac
      241 atgacccgct acgaacgcat cgcggccatc gacacgcctc agtactatgc cgactggcac
      301 ggacgcgatg cggccttgct ggtctacacc tccgagccgc tgcccgagcc actggtcttg
      361 gccggccacg ccgtcgtgag cctgtggctc gaggccgatc agccggatgc ggccatccac
      421 gtctacctcg aggacatcgg tgcggacggg cgctgccgct acgtgaccga gggggcgttg
      481 cgggccttgc accgccagac ggcgccctgt cccgcgcacc accgcacgag ctggccgtgg
      541 cgcaccttcg cgcgcgccga tgccgccctg ctgccgcggg gtgaggtcgt ggaactgacg
      601 tttgcgttgc tgccggtcgc ctgggccttc gcagccggca gccgcctgcg ccttgccatt
      661 tcgggggccg actgcgatca cgtcgtgcag gtcccgcacg gccgaccacc tctgttgcgc
      721 atccatcacg gcagccggca cccctcgcgc ctggcgctgc cctgtctttc cggcggagcc
      781 tcggcgctat aaggggtaag gcgtccggcg ccgcgagagg cgcgcgcccg ggagatggca
      841 tggccctact gcaggtgatc ggcgtcgagc acgccttcta tggcgtggag gccctcagcg
      901 gcgttgacct cgccgtggag ccgcacaccg tcacgggcct gatcggcccc aatggcgcgg
      961 gcaagaccac gctcttcaac tgcatcacgg ggattgtgcc gcccgatcgc ggtcgcatcc
     1021 ttttcgatgg ggccgatatc gccggtttgc gccccgacca ggttagccgg cggggcttgg
     1081 cgcgcacctt ccaaatcgcc cgtggcctgc cacgcctttc cgtcatcgag aatttgatgc
     1141 tctacggccc gcgccagccg ggcgagcgct tgctgccggc gctgctgcgg ccggcatcgg
     1201 tgcggcggcg cgaggaggcc cttgtcgccc gtgcggtcgc catcgcgcgg cggctcaatc
     1261 tcttttcggt gatcgccaac cctgcctccg acctctcggg cgggcaaaaa aagctgctcg
     1321 agatcggccg cgtcctgatg tcggagccca agctgatcct gctcgacgag ccggtggccg
     1381 gcgtcaatcc aacacttgcc cgcgaaatcg gcgcccacct cagggcgctt gccagcgagg
     1441 gactgacgat cctgttgatc gaacatcact tggacatggt ggctcggctc tgcgaccacg
     1501 tcatcgtgct ggcggaggga cggcgcctgg ccgaaggctc gttcgccgcg gttgcggccg
     1561 atcctgccgt gcaggaggcc tatatgggcc ggcggcgcgc gggtgtgccc tagacatgag
     1621 cctgctgatg gcaaacggtg tggtggccgg ttacggcgcg caggatgaga tcctgaaagg
     1681 cgtcgacctc gtcgtcgatg cgggcgaagt cgtctgcatt atcggcccca acggggcggg
     1741 taagtcgacg ctgctcaagg cgatcgcggg cctgttgcgt ccaaagctcg gcagcgtgac
     1801 gctcaggggg cggtccattg gcggcttgcc ggcccgcgag atttcgcggc tcggcctcgc
     1861 ctacgtgccg caggagttca atgtctttcc gaccctgacg gtgcgcgaga acctggagat
     1921 cggcggctac atcgaccgca aggcggcggc gcgccgcatc gagggcgtgt tggcccgttt
     1981 cccggtgctg gcggacaagg cgcgccacgc cgcgcgcacg ctgtctggcg gcgagcgcca
     2041 aatgctggcc atggccatgg cgctgatggt ggaacccgtg gtgctgctgc tggatgagcc
     2101 gtcggccggc ctatcgccgc gcgcggccga gcgcctgttc gacgccgtcg tcgagatcca
     2161 caaggacggc gtggcggtgg ccatcgtcga gcagaatgcc aacgaggcgc tcgccatcgc
     2221 cgatcgggcc tacatcctgg tcgacggcaa gaatagtcgc accggtgagg cccgcgcgct
     2281 ggccgcggat ccagagatca gaaggctctt tctcggggcc tgacatcagc ctagtatttt
     2341 cgttcgatct cgccagaggg gtttgccatg ccaggaccga cacgccgcgc gctcatcacg
     2401 ggtgccgccg cggccagcgc cattgccgcc tcccctttcc cgagcgtcgc ccagagcgac
     2461 cccatcaagc tcggcgtgct cacgcccctg accggggccg gcggccagga cggcccgcgc
     2521 atgctcaagg ccatggaggc cgtgatcgcc gaggtcaaca aggccggcgg cgtgcttggc
     2581 cgcaaactcg aaaccgtcgt cgaggacgat cagaccaatc ccgaagccgc ggtccgcgcc
     2641 gcccgcaaac tgatcgaggt caacaaggtg ccggtgatca tgggcacttg ggcctcggcg
     2701 gtcaccagcg cggttgcgcc cgtgtgctgg gaagcaaaga cctttctgtg cacggtctcc
     2761 ggggccgatt ccatcacgca gctgccgcat caaggctacc tcatccgcac gcagccgaac
     2821 tccaagctgc aggcgaccgc gcatgcggac ttcattgcgg ggctaggcaa gaagaaggtc
     2881 gccgtgatcg gcgtgcaggc acccttcgcg ctgtcgacca aggagcacct ggacgcggtc
     2941 ctcaagacca agggtgccaa ggtcgtcgcc cacatcattt atgaaaagga caaggcgacc
     3001 tatcgctcgg aaatcgacca ggcgatgcgc tcggatcccg attttctcta cctcaacggc
     3061 tatgcgccgg acaccgtggt ggtgttgcgt gatctctaca aggccaacat caatctgccc
     3121 aagttctgcc agtcctacgc ggtgccgcaa aagacgctcg ataccatccc ggcggaggtg
     3181 tcggcgggca cctacaccgg gcagccctcg gccgatatcg agggcaaggc cttcaaattg
     3241 gcggcggccc ggctcggcat cgccgagccc gattcctatg aggcgcaggc cacggattgg
     3301 gcgagcctcg tgatcttgac gctggccaag gccaaggagg tcaccggcac ggcgctgaag
     3361 gaaaacgtgc gcaagatcag ccagggcagc ggcgagaagg tttggagcgc ggttgagggc
     3421 ttgcaggcgc tggccgcggg taaggaaatc aactacgagg gggcctcggg accctgcgac
     3481 ttcaccgaga ttggcgatat cgagggttgc cgcttccgct acatggaggt ggtggatggc
     3541 aaattgcgct tcctcaaggt ggtctagcca gcgggcgtga gctctcgagc gcgaagttca
     3601 agcggcccgc atcatggaag tctggcagct gatcctgaat gggttgatgt cgggaaccat
     3661 cctggccgtg ccggccatcg gcctcagcgc catcttcgcg gtgctgcgct ttaccaactt
     3721 cgcgctcgcc tcgcacatga cgattggggc cttcgccggc ttcgtcgcca atgtctggct
     3781 cgcctggccc gtggttccct cgctgctcgc ggccttcctg atcgccgggc tcgtcggcgt
     3841 gctgtcggat gaattcatcc tcaagccctt tcgggccgcg ggctttatca ccaccgcgat
     3901 cggctcgatc gcgctcacga tcgcgctcga gaacggcgtg cgcttcgtct ttggcaacga
     3961 gctcaggggc tacgacctgc cgatccggcg cgattgggtg ctcggtgggc tccgcgtctc
     4021 cccgcaacag ctggaaaacc tcctgatcgc gctcgccgcc atgggcctgc tgttcgcctt
     4081 tctgttcttt acccgcacgg ggcgcgccat gcgtgccttt gccgacaatc cgatgctggc
     4141 gtccatcaaa ggcatcaatg ccgacatggt ggcgcgtctg gtctcgttcg tggccatggg
     4201 gctggcgggg ctcggcggca tgctgatcgg gctcgatacc accatcgatc ccctggtggg
     4261 cttcaagagc atcctgacga ttttcgcggc cgccgtggtc ggcgggctcg ggagcctgcc
     4321 cggcgcggtc gttggcgcct tgctggtggg cgttggggag gaattgagtt tgctgatcct
     4381 gcagccggcc taccgcagcg ccatcggctt tctcgccatc ctgatggtgt tgacgctcag
     4441 gccgcgcggg attttgggcg cgcgggcgta ttgaggagag cacatggagc actacctcgt
     4501 cgccatcggt gtcaccgccg gcgtctacgc gctgatggca ctgggcatga acgtcatttg
     4561 gggcatggcc ggcttgatca acttggggtt ggtcggcttc ttcgccgttg gcgcctacgt
     4621 gtcggggctg ctgacgctca aactcggtgt gccgattgtt atcggcgtgg cggcgggcac
     4681 ggtcatggcg gcgctggtcg gcgtgctggt ggcgctcatt accgtcaact tgcgcggcga
     4741 ctatctggcc atcgtcacgc ttgggttcgc cgagagcctg cggatcgtca tgtcgaacga
     4801 gctgtggatt gccaatggca ccgatggtct ttcgggcatt ccggggccct atcgtgcgga
     4861 actcgggccg caccttttca acctcttcta ccttgcgatc gtggtcggcg cggtgatcgt
     4921 cttgtttttt ctggtcgacc gcttggtcaa ttcgccgttt gggcgcgtgc tgcgcgccat
     4981 tcgcgacgat gacgtgattg ccgaggtcgc cggcaaacac gtcgccgtct tcaagatcaa
     5041 ggcctttgcc ttcggcagtg cggcgctcgg cctggccggc gcgctctacg ggcattttac
     5101 gagctacatc gcgcccgatc tgttcgcgcc gctgctgacc ctctatgtca agctatcgct
     5161 gctgaccggt ggtctcggca acagcaaggg tgcggtgctc ggcgccgtgc tcgtggtctt
     5221 ctttttggaa tcgacgcggt ttctggcgcc cttgatcccc gggctgtcgg ccgtgcaagc
     5281 cgccgccgtg cgcgagctgg cgatttccgt ggccctgctg gtgatcttgc gcttttacgc
     5341 taggggcttg ctgcccgagc gcatcgagcc gccggcgctg gcacccgagg cggccgcacc
     5401 cgccctcaat cgcatttgat cgaccgaaag aggcaaaacc catgcgcaag accaagcgtg
     5461 gcctggagat ggccgatgtc gccgccatcc tcgagcgctc cagcgaggct ggcgacgtgc
     5521 gcgaggtcta tcgcgaaatc gagcgcatct cgagggagtg cgtgggctgg cgcctgctga
     5581 cgatcctgcg ctatgtcgag gccgatgcgg ccgtcgagcg cctctactcc agcgacgaaa
     5641 aagcctaccc ggtcggtggt cgcaaacccc tcgacaagat cgccaccagc cacgcggcca
     5701 tggagaaggg cgacgtcttc ctggcggcga cgcgggagga ggtccgcacc gccttcttcg
     5761 accacgagct gatcttttcg ctcggcatca ccgccatcct caacgcaccg atccggcagg
     5821 gtgggcgaag gctcggtacg ctcaacttct gcggcgagga aggcgcctac ggcaccgccg
     5881 aggtcgcgtc cgcccggctc ttggctggcc tcgtcgcgcc gtccctgctg cgcggcggcg
     5941 attgacgccg tgcggcaatt taccgatgct cgcgcatggg tcacagggaa caagggcgat
     6001 gacgcaatat cacttcaaaa acttcgagtt gctcgacccc gaacgcgagg agctcgtggg
     6061 tggccacgaa cttctcgtcg aaggcgcctt gatccgtgag ctttccgcca agccgatcaa
     6121 ggcgccggag gcgaacgtga tcgattgcgg tcagcgcacc ttgatgccgg gcctgatcga
     6181 cagccatgtg cacgtggtgc tctcggaggt gtcgctgccg cgcctcgagc acgtaccgct
     6241 gaccttgatg acggcacgcg ccgcggtgct gatgcgcaac atgctcgacc gcggctttac
     6301 cagcgtgcgc gacaccggcg gcgccgattg gggcctcaaa gaagcagtcg acaaatggct
     6361 gctgccgggg ccgcggctgt tcatcgccgg caaggcgctc ggccccaccg gtgggcacag
     6421 tgatggccgg cggcgcacgg atcacggcac gcgttgccac tgctgcgatg ccttgcattt
     6481 tgccatgggc ctggccgatg gcgtcgccga ggtacgcaag gccgtgcgcg aggaaatgcg
     6541 ccagggctgc gatcaggtga agatcatgat gtcgggtggg gtggcctccc cctatgaccc
     6601 cctggacagc ctgcagttct cccccgagga ggtggcggcg gcggtcgagg aggcgcatgc
     6661 cttcgggcgc tacgtctgcg cccacgccta tagcgccgag gcgatcacgc gggccgcgca
     6721 cgccggcgtg cgcaccatcg agcacggcaa tctgatcgac gaagcctcgg ccaagttgat
     6781 ggcgtccaag ggcatgtacc tgatcgccaa cctcgtcacc tattatgaga tgaagaagcg
     6841 cgcggccgaa tacggcatga gcgcggacat gctggcgaag aatgacctcg tcatcgacgg
     6901 gggcctcagg tcgcttgaga tctgcaagcg cgcgggtgtg cccgttgcct atggcacgga
     6961 tctgttggga caattgcaga gcgaacagtc gcgcgagttc atgatccgcg cggaagtgca
     7021 aaagccgatc gaggtcattc gcgccgccac gctgatcggc gccgagatcg tgcgccaggt
     7081 gggcaagctc ggctgcctca agcccggtgc cttcgccgac ctgctgctgg tcgacggcaa
     7141 tccgctgctc aatttgggtg tgctgcagga gcaaggcaag tttctcgccg ccatcatgaa
     7201 gggcgggcgt tttcacaaga accgactgca ttaggcgatg gcgcaacttc acttcaagaa
     7261 tttcaggttg ctcgagcccg cttatggcga gctgcgcgga ggctacgagc ttctgatcga
     7321 gggcgagcgc gtgcgtgagc tgagcgagcg cccgatcgtc agccgcgctg ccgaggtcgt
     7381 ggattgcggt gggcgcacgc tgatgcccgg tctcatcgat tgccacgtgc acgtgattca
     7441 tagcgaggtg aacatccgct ttttggaggc catgccgctg acgctgctta ccgcgcgggc
     7501 ctcgctgcgc ctgcgcgcca tgctcgatcg cggttttacg accgtgcgcg acaccggcgg
     7561 cgccgattgg ggcatcaaga ccgcgctgga gcagggttat ctggtgggtc cacgcctcta
     7621 tattgcgggc atgtcgatcg gtcccaccgg cggccattcc gatggccggc gccgtaccga
     7681 tgacagcgac ggctgcagct gctgcaatgg gctcaaattc aagtcggcgg tggccgacgg
     7741 taacgacgag gtgcgcaagg cggcccgcga gcagatgcgg cagggggcgg accatgtgaa
     7801 gatcatgatg tcgggcggtg tcgcctcgcc ctatgacccg ctcgacagtc tacagttctc
     7861 agaaggcgaa gtggccgccg tcgtcgagga ggcgcacgcc ttcggccgct atgtctgtgc
     7921 gcacgcctat tcggcgcagg ccatcacgcg tgcggcgcgg ctcggcgtgc gcaccatcga
     7981 gcacggcaat ctgatcgatg acgcggcggc gcggctgatg gcgtcgaagg gcatgttcct
     8041 ggtggccaac ctcgtcgcct actatgccat gaaggagcgg gcgcaaaagt tcggcatgag
     8101 tgccgagagt ctggccaaga acgagatcgt gctggagggc ggtctcaagt cgcttgagat
     8161 ctgcaagcgc cacggcgtgc cggtcgccta tggcagcgac ctgttggggg agctgcattg
     8221 ggatcagagc cgcgaattca gcttgcgccg cgaggtggtg agcccgatcg agatcatccg
     8281 gtcggcgacc acgattgcag cgcaagtctt gcgtctggaa ggcaaggtcg gcaccttgct
     8341 gcccggcgcc tgggccgatt tgttgctggt cgacggtgac cccttgaaag atctagccgt
     8401 gctcgaggat cagggtcgcc atctctccat catcatgcag gccggtcggt tccacaaacg
     8461 ccggctgcat tagacgctga gccatgggcg cgccgctcaa agccttgcag gacaggggag
     8521 ggcagcagat gcggcccggc atggccaatg cgcgctcgct gggccgcctg gcgctgaagg
     8581 ccggcgcggg cgtgaccctt ctctacattc tgacgccttt gctgttcgtg acttggctgg
     8641 ccttttttaa gcaggagatt ccatcctttc cgcccgaggg ctactcgttg cgctggttcc
     8701 tggcgatccc cggcaatgac cgcttcgttt ccgggttcct cttgagcctc gaggtcgccg
     8761 tgatcgccac cgccatcggc ctcctgctcg gc
//
LOCUS       DASTMT010000309         3246 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_171550, whole genome shotgun sequence.
ACCESSION   DASTMT010000309 DASTMT010000000
VERSION     DASTMT010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3246
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_171550"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..281)
                     /locus_tag="VFR00_07250"
     CDS             complement(<1..281)
                     /locus_tag="VFR00_07250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0159091.1"
                     /translation="MRATPDQLVFDLPQRPALGAQDFLISASNQAAAAMIDRWPHWSD
                     HAVLVLAPARAGKTHLANVWRRKSGAAALIGARLSEADVARAGRAILVE"
     gene            complement(385..954)
                     /locus_tag="VFR00_07255"
     CDS             complement(385..954)
                     /locus_tag="VFR00_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008961176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEU0159092.1"
                     /translation="MLLLANIPNAITLGRVMSVPVLFYLLVSGRSVAAFVVFLLAGLS
                     DAVDGYLAKRFDWRSELGAYLDPLADKLLIVSIFIALGVRNELPLWLVIAVVSRDILI
                     VVAVLLALVLDQPIPIKPLAISKLNTVAQIVLAAAVLANDAFALQLEASRSALVWITG
                     ALTLLSLAAYLRDWFMHMTGYETGKGPPA"
     gene            complement(1061..2203)
                     /locus_tag="VFR00_07260"
     CDS             complement(1061..2203)
                     /locus_tag="VFR00_07260"
                     /inference="COORDINATES: protein motif:HMM:NF021367.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2066 domain-containing protein"
                     /protein_id="HEU0159093.1"
                     /translation="MRPLDLKTGGLSAVAQGRRRSLILTVAGTLLGTAAGSVAGLPLA
                     VAAEAVFTVGNYPVEARADNAVAAKDRALNEGQQAAFRSLLRRLVPVTAFARLKQLPS
                     VRAGDLIEGVKVRAERNSATDYIANLDFSFQSKGVRDLLRREGIPFTDEQAPPVTLIP
                     VWVTPGAPAKTDAAWSAVWNGLDLEHALTPGKLQPLKPEVTGETVNALIAGDGGALRS
                     LARAYQSELVLLAAAAPDPAQGRLNVTLTGRDAVGAFTLKRAFRLDRTDPAYTSELAA
                     VVLLGILEGRWKALRAGGAGGTAAAAAGDLLIAVEFRGMGEWQEISRKLSAIPGVEEL
                     EVAGLSARGARVTLRYPDGAERLAQSLSQQGLGLRNVAGKWVLSLQ"
     gene            2434..>3246
                     /gene="purM"
                     /locus_tag="VFR00_07265"
     CDS             2434..>3246
                     /gene="purM"
                     /locus_tag="VFR00_07265"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006331904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEU0159094.1"
                     /translation="MPSRPPQEKPHTYRDSGVDIDVGNAIVEAIKPLAKATLRPGADT
                     QLGGFGGLFDLKAAGFKDPILVAANDGVGTKLKIAIATGQHATVGIDLVAMCVNDVLV
                     QGAEPLFFLDYFASGKLDLATAQAVIGGIGEGCRLAGCALIGGETAEMPGLYAGQDYD
                     LAGFCVGAVERGQLLPRPDIVPGDALIGLAAAGVHSNGYSLVRRLVAAAGLAWDAHAP
                     FNAARPLAEALLEPTRIYVKAALRAIRDTGAVKAMAHITGGGLSENVPRVLPD"
BASE COUNT          552 a         1114 c         1069 g          511 t
ORIGIN      
        1 tcctccacca agatggcccg ccccgcacgc gccacgtcgg cctccgacag gcgcgccccg
       61 atcagggccg cagcaccgga tttgcggcgc cagacgttgg ccaggtgcgt cttgccggca
      121 cgcgccggcg caagcacgag cacggcgtga tcgctccagt gtggccagcg gtcgatcatg
      181 gccgcggcgg cctgattgga agcactgatc agaaaatctt gcgcgcccag agccgggcgc
      241 tgtggcagat cgaaaacgag ctgatcgggc gttgctctca tgacgcgggg accgttttga
      301 aggatgctga agaggatgct gtgatgcggt gcaccgtgcc tgctcgcctt ggctgtcgtc
      361 cccggccgcg caggggatgc cggctcaggc cggtggtccc ttgccggtct cataacctgt
      421 catatgcata aaccaatccc gcaggtaggc ggccagcgaa agcagcgtca atgctcccgt
      481 aatccacacc agcgccgatc gtgaagcctc gagctgaagc gcaaaagcgt cattggcgag
      541 cacggcggcg gcgagcacga tttgcgccac cgtattgagc ttgctgatcg ctaggggttt
      601 gatgggaatc ggctgatcga gcaccaaagc cagcaaaacg gcgacaacga tcagaatgtc
      661 gcggctgacg acggcaatga ccagccagag cggtagctcg ttgcgcacgc cgagtgcgat
      721 gaaaatactg acgatcagca gcttgtcggc caacggatcg aggtaggccc cgagctcgct
      781 gcgccagtca aagcgcttgg cgagatagcc atcgacggcg tcgctgagac cggcgagtag
      841 gaacacgacg aaggcggcca cgctgcgccc cgataccaac aaataaaaga gtaccggcac
      901 ggacatgacc cgcccaagcg tgatggcatt ggggatattc gcgagcaaga gcaccccagg
      961 cgttgctgtc ggcccgcccc ggcaccccgc cgcgggcggc gagcacggag gaagcctctg
     1021 gcggcaatgc taggcccggc ccacgcgcgc cgtcgagcct ctattgcaag gacaggaccc
     1081 acttgcccgc gacgttccgc agccccaatc cctgctgcga caggctttgg gccagacgct
     1141 ctgcgccatc cggatagcgc aaggtcacgc gcgcgccgcg cgcggacagg cccgcgacct
     1201 ccagttcttc cacgccggga atcgcgctca gtttgcggct gatctcctgc cattcgccca
     1261 tgccgcgaaa ctcaaccgcg atcaacagat cccccgcggc cgcggccgcg gtcccgcccg
     1321 caccgccggc gcgaagggcc ttccagcgac cctcgagaat gcccaagagc accacggcgg
     1381 ccaattcgct ggtgtaggcg ggatccgtgc gatcgagccg aaaagcccgc ttcagcgtga
     1441 aggcgccgac ggcgtcgcgc cccgtgagcg tcacgttgag acgcccctgg gcagggtccg
     1501 gcgcggcggc ggcgagcagc accaattcac tttggtaagc gcgcgcgagg ctgcgcaagg
     1561 cccccccgtc accggctatg agggcgttca cggtctctcc cgtcacctcc ggcttcaagg
     1621 gttgcagttt gcccggcgtc agggcgtgct cgagatcgag cccattccac accgcgctcc
     1681 aggcggcatc cgtcttggcg ggcgcaccgg gcgtcaccca gaccgggatc agtgtgacgg
     1741 gcggtgcctg ctcgtccgtg aaggggatgc cctcgcgccg taacaggtcg cgtacccctt
     1801 tcgactggaa ggaaaaatcg aggttggcga tgtagtcggt ggccgaattg cgctccgcgc
     1861 gcaccttgac accctcgatc aagtcgcccg cgcgcacgct tggcagctgc ttgaggcgcg
     1921 caaaggcagt gaccggaacc agccggcgca gcagggaccg gaaggctgcc tgttgaccct
     1981 cattcagggc ccgatccttg gccgcgacgg cgttatcagc ccgggcctcg accggatagt
     2041 tgccgaccgt gaagacggcc tcggccgcta ccgccagcgg gagccccgcg accgacccgg
     2101 ccgccgtccc caggagggtg ccggcaacgg tcaagattag gctccggcgc cggccctggg
     2161 ccacggcgct cagcccaccc gtcttcaggt cgagagggcg catgacttcg tttccagcac
     2221 gtgttcctcg cacgcttcgt tccgctcctt gcggccggcg ccgccgccca taggccgctg
     2281 cctgcctcct tgggccggcc ctgctgggcc ggcgctcggc ggccccgagc agcctctagc
     2341 catccccagc gctcgtgccg caaaccctac cgctgcaagg atcggtctgc tatgccgcag
     2401 catcttaggc accaataggc aagctttaag gccatgccaa gccggccccc ccaggagaag
     2461 cctcacacct accgcgattc cggcgtcgac atcgatgtcg gcaatgcgat cgtggaagcg
     2521 atcaaaccct tggccaaagc gaccctgcgg cccggcgcgg atacccagct cggtggcttt
     2581 gggggcctgt tcgacctcaa ggccgcgggg ttcaaggatc cgatcctcgt cgccgccaac
     2641 gatggcgtgg gcaccaaact caagattgcc atcgccacag ggcagcatgc cacggtcggc
     2701 atcgatctcg tcgccatgtg cgtcaacgat gtgttggtgc aaggggccga acctttgttc
     2761 tttcttgatt actttgccag cggcaagctc gatctggcca cggcgcaggc cgtgatcggg
     2821 ggtattggcg agggttgcag gctggccggt tgcgccctga tcgggggcga gacggcggag
     2881 atgcccggcc tttatgccgg gcaggactat gacctcgccg gcttctgcgt gggtgcggtg
     2941 gagcgcggcc agctgttgcc gcggcccgac atcgtgccgg gcgacgccct gatcgggctc
     3001 gctgccgccg gcgtgcactc caacggctac tccctggtgc ggcggctggt ggctgctgcg
     3061 gggctggcct gggatgcgca cgcgccgttc aatgcggctc gcccgctggc cgaggcgctt
     3121 ctggaaccaa cgcgcatcta cgtgaaggcg gcgctgcggg cgatccgcga caccggcgcc
     3181 gttaaggcta tggcccacat caccggcggc ggcctgtcgg agaacgtgcc gcgtgtcctc
     3241 cccgac
//
LOCUS       DASTMT010000310         2142 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_1812600, whole genome shotgun sequence.
ACCESSION   DASTMT010000310 DASTMT010000000
VERSION     DASTMT010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2142
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1812600"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            79..993
                     /locus_tag="VFR00_07270"
     CDS             79..993
                     /locus_tag="VFR00_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU0159095.1"
                     /translation="MTEALPFKTTMTFAYGEPRQLAPGVVRIVANNPNHFTFKGTNTY
                     LIGTDPVALIDPGPDDPAHLAAILRALDGRPLSHILVTHTHHDHVDGLPALMAATAAQ
                     TVGYGHKARNRGAKRTSAAGGEFVEEDFVPDLAMKHGDRLSGEGWSFEALFTPGHAPD
                     HLCFALTGTDILFSGDHVMGWNTSVVAPPEGNMAAYVRSLELLTQRPERTYFPGHGGQ
                     IEEPQRLVKAYLLHRRMREHAILDCIRQGHTTVEAIVPVVYRGLDSKLLNAAALSVLA
                     HVEHLKERGLVAYEAPLSASRVLSAASS"
     gene            complement(878..1816)
                     /locus_tag="VFR00_07275"
     CDS             complement(878..1816)
                     /locus_tag="VFR00_07275"
                     /inference="COORDINATES: protein motif:HMM:NF019032.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1499 domain-containing protein"
                     /protein_id="HEU0159096.1"
                     /translation="MIDHGHKIHSRLARWSGRLALFSAGLTVAGVALHRLTSFPTPTA
                     INLFAVSALGLALAALIGLVSLVGIWRKGYAGAGTAFLGMFLALLFLGWPLTYLPALF
                     KLPRINDVSTDLSSPPRFEALAPQRAAGAKPTAYPGEPVALLQQAAYPDLRGITLERS
                     AEETFELVEEAVRKLKWKVAASEAPTLKSGRPGIIEATDQTLLLGFPDDVAIRVIGNN
                     TRSRIDIRSASRYGEFDFGQNATRIRRFYGEVQAQIDATLPTTVAGRRRLRTTRGAAM
                     MKRLKERETPKVAPRTPRDRAPSSAQRERAPKEPQR"
     gene            1922..>2142
                     /locus_tag="VFR00_07280"
     CDS             1922..>2142
                     /locus_tag="VFR00_07280"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU0159097.1"
                     /translation="MSNAPPDPSALADKDAAAFGFRQVAAETRQGLVNQVFSTVAARY
                     DLMNDLMSGGLHRLWKDDLITWLNPPRSQR"
BASE COUNT          361 a          743 c          682 g          356 t
ORIGIN      
        1 tggcttgcgc gccaagccgt tggcggctag gcccaggtgt gcgccaagcg ctattcctcc
       61 tctgacgtcg ggatcctgat gaccgaagcc ctccccttca agacgacgat gacctttgcc
      121 tatggcgagc cgcgccagct cgccccggga gtggtgcgga tcgttgccaa caaccccaac
      181 cacttcacct tcaagggcac caacacctat ctgatcggca ccgatcccgt cgcgctgatc
      241 gaccccggtc cggacgatcc ggcgcatctg gcggcgatcc tgcgggcgct cgacggacgg
      301 cctctctccc acattctcgt tacccatacc catcacgatc acgtcgacgg cctgccggcg
      361 ctcatggccg ctaccgccgc gcaaacggtg ggttatggcc acaaggcgcg caatcgcggc
      421 gccaagcgca ccagcgccgc gggaggcgag tttgtggaag aagatttcgt gcccgacctc
      481 gccatgaaac acggcgatcg gctcagcggc gagggctggt cgttcgaggc cctcttcacg
      541 cccgggcatg ccccggacca tctctgcttt gccctgacgg ggaccgacat cttgttttcc
      601 ggggatcacg tgatgggctg gaacaccagc gtggtggcac cgcccgaagg caatatggcg
      661 gcctacgtcc gctccctgga gctgctgacg caacgccccg agcgcaccta ctttccgggc
      721 cacggcggcc aaatcgagga gccgcagcgc ctggtcaagg cctacctttt gcatcggcgc
      781 atgcgcgagc acgccattct cgactgcatt cgccagggtc acaccacggt cgaggcgatc
      841 gtgcccgtcg tctaccgcgg cctcgactcc aagctcctca acgctgcggc tctttcggtg
      901 ctcgctcacg ttgagcactt gaaggagcgc ggtctcgtgg cgtacgaggc gccactttcg
      961 gcgtctcgcg ttctttcagc cgcttcatca tagccgcgcc gcgggtggtg cgcagccgcc
     1021 gtcgacccgc caccgtcgtc ggcaacgtgg catcgatctg tgcctgcact tccccataga
     1081 agcgacgaat gcgcgtggca ttctggccga agtcgaattc gccgtagcgc gaggcggagc
     1141 gaatgtcgat gcgcgagcgc gtgttgttgc cgatgacgcg aatggcgacg tcgtcgggaa
     1201 agcccaacag cagcgtttga tcggtggcct cgatgatgcc gggccggccg ctcttgaggg
     1261 tcggcgcctc ggatgcggcg accttccact tgagcttgcg cacggcctct tccacgagtt
     1321 cgaaggtttc ctccgccgag cgctcgagcg tgatgccgcg caagtccggg taggcggcct
     1381 gctgcaacag ggcaacgggc tcacccggat aggcggtcgg cttggcgccg gcggcgcgct
     1441 gcggcgccag agcctcaaag cgcggcggcg acgacagatc ggtcgagacg tcattgatcc
     1501 gcggcagctt gaacagggcc ggcaggtagg tcagtggcca cccgagaaaa agcagcgcca
     1561 ggaacatgcc aaggaacgcc gtgccggcgc cggcgtagcc cttgcgccag atgccgacca
     1621 gcgacaccag gccaatgagg gcggcgagcg ccagccccag ggcgctgacg gcgaatagat
     1681 tgatcgccgt cggtgtcggg aacgaggtta gccggtgcag ggccacgccc gcgacggtca
     1741 gcccggccga aaacagcgcc aggcggccgg accagcgcgc cagccgtgag tggattttat
     1801 gcccgtggtc gatcatggcc cgccatctgc tagcttcggc tcatagcaat gttggggcaa
     1861 gactgccagc acccgccctc tggccgcgcg cccgggcctc ggctagggtc cgcaaagcgc
     1921 catgagcaac gccccacccg atccttccgc ccttgcggac aaagacgctg ccgccttcgg
     1981 cttccgccag gttgcagccg aaacccggca gggccttgtc aaccaggtct tttccacggt
     2041 tgcggcccgt tacgacctga tgaacgacct gatgtccggc ggcctgcacc ggctctggaa
     2101 ggacgatctc atcacctggc tgaacccgcc ccgctcgcaa cg
//
LOCUS       DASTMT010000311         3359 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_3453987, whole genome shotgun sequence.
ACCESSION   DASTMT010000311 DASTMT010000000
VERSION     DASTMT010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3359)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3359)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3359
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3453987"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..561
                     /locus_tag="VFR00_07285"
     CDS             <1..561
                     /locus_tag="VFR00_07285"
                     /inference="COORDINATES: protein motif:HMM:NF014665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0016653 - oxidoreductase activity, acting
                     on NAD(P)H, heme protein as acceptor [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HEU0159098.1"
                     /translation="LHLGLGVLILAALLWAALSLRARAQGAPSQAPARGATAIVLLVF
                     LQVILGALVAGLKAGHAYNTWPLMDGRLIPSGLWVIEPWYLNLFENALTVQFNHRLVA
                     YALLLAVFWRALVLARPRRQPGARVSAGCLALAVTAQLGLGVYTLISQVPPLLGLAHQ
                     AMAAVLFALAVWHLFETRHPRLASPC"
     gene            555..1238
                     /locus_tag="VFR00_07290"
     CDS             555..1238
                     /locus_tag="VFR00_07290"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEU0159099.1"
                     /translation="MLNLVLVFDLDDTLYPERQFALSGFAAAELWAQTSLGLTGLAAD
                     MTRLLDQGHLGQLFRLVLEARMPEHRPEHLAGLLQAYRSHVPSLMLFEDAAWALSHFG
                     GQLRLGLITDGTHSVQRRKVEALGVARRFREIVYTDALGGRAFAKPHQQSFQMMEAAL
                     GGDADRFVYVGDNPAKDFVAPNARGWVTVMVDRPGALKIHAGAKPAEGGRPQHTIASL
                     RELTAVLKL"
     gene            1341..2294
                     /gene="ubiA"
                     /locus_tag="VFR00_07295"
     CDS             1341..2294
                     /gene="ubiA"
                     /locus_tag="VFR00_07295"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HEU0159100.1"
                     /translation="MSSKPPSPAHAVLPDALEGNWVDRLAPASCRPFLRLARLDRPVG
                     GWLLLFPCWWGQLLAEGALGRTWADAFYLWLFLIGAFVMRGAGCTYNDIVDRNYDVRV
                     ARTAARPIASGQVSLVEAQLFLGVLSLVGLLVLVQFNLFTILLGASSLVLVALYPFAK
                     RYTDWPQVMLGLTFKWGALVGWSAVAGSLSAAPLMLYLGCVLWTVGYDTIYAHQDKED
                     DLAIGLRSTALKLGDATQSWLVFFYAGAAVAWAAAGVLAGAGALYFLALLLVCAHFGW
                     QITTLVVADAANCLARFRSNQLIGWMLCLGLVLDLLMKAHT"
     gene            2548..2808
                     /locus_tag="VFR00_07300"
     CDS             2548..2808
                     /locus_tag="VFR00_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009798811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4170 domain-containing protein"
                     /protein_id="HEU0159101.1"
                     /translation="MPPVSTPRPAKQLLHLVFGGELKTLTDVEFKDLDRLDVVGIYPS
                     YAEAYKAWKAAAQRSVDDAQMRYFIVHIHRLLDPTPGVGAEP"
     gene            2908..>3359
                     /locus_tag="VFR00_07305"
     CDS             2908..>3359
                     /locus_tag="VFR00_07305"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HEU0159102.1"
                     /translation="MRKTSNLAGRKAKSLALSARERGLLRRFLADWIRPRWRQIVLAL
                     LFTGGLAAATAGYPLIIKSAFDSLMQPGSLALPWVLVSVIGITACRGLFLYLHQITTA
                     RIVTRMVTDIQKTAFAHLINGDFARFGRETTGHLVSRLTNDLTFIQQAA"
BASE COUNT          498 a         1100 c         1047 g          714 t
ORIGIN      
        1 ttgcatttgg gtctcggtgt tctcattctg gcggccctgc tgtgggcggc cctgagcttg
       61 cgcgcgcgcg cccaaggcgc gccatcccaa gcccccgcac gcggcgcgac cgctattgtc
      121 ctgttggtct ttctgcaagt catcctcggc gccttggtgg ccggtctcaa ggccggccat
      181 gcctacaaca cgtggccgtt gatggacggc cggctgatcc cctccgggct gtgggtcatc
      241 gagccctggt acctcaactt gttcgagaac gcgctgaccg tgcaattcaa tcatcggctc
      301 gtggcctacg cgctgttgct ggctgtgttc tggcgcgccc tggtcctggc ccgcccgcgg
      361 cgacagcccg gtgcacgggt ctctgccggc tgtttggcgc tggctgtcac ggcgcagttg
      421 gggctcggcg tctacaccct catcagccag gttcccccgc tgctcggtct cgcgcaccag
      481 gccatggcgg ccgttctatt cgcactcgcg gtctggcacc ttttcgagac gcggcatcct
      541 cggctggcgt cgccatgctg aacctcgttt tggtcttcga cctcgacgat accctctatc
      601 ccgagcgtca gtttgcgctt tcgggatttg ccgcggcgga gctgtgggcg caaacgagcc
      661 tcggtctgac cgggttggcc gcggacatga cgaggctttt ggaccagggt cacctcggtc
      721 agctgtttcg cctggtgctc gaggcgcgca tgcccgagca ccgaccagag cacctggccg
      781 gccttttgca agcctaccgt agtcacgtgc cgagcctgat gctgtttgag gatgcggcct
      841 gggcgctcag ccactttggc ggccagttga gattgggctt gatcaccgat ggcacccaca
      901 gcgtgcaaag gcgcaaggtc gaggcgctgg gcgtggcgcg gcgctttcgc gaaatcgttt
      961 ataccgatgc gctcggcggt cgcgcctttg ccaaacccca tcaacaaagt ttccaaatga
     1021 tggaggctgc gctcggcggc gacgccgacc gtttcgtcta cgtcggcgac aacccggcga
     1081 aggatttcgt tgcccccaac gcgcgcggct gggtgaccgt gatggtcgac aggccggggg
     1141 cgctaaagat ccacgccggc gccaagccgg ccgagggcgg tcgaccgcag cacaccattg
     1201 cctcgctgcg ggagctcacc gccgtgctca agctttagcc gatctcgtgg cgaaatggcg
     1261 cgtgctataa tcggccccca cacccgcctg gtgcgcgggt ttgagcgtca aaggcgcggc
     1321 agcgagcaac ttcgccaacc atgagcagca aacctccctc gccggcccat gcggtcctgc
     1381 ccgacgcgct cgagggcaac tgggtcgatc gccttgcgcc tgcgagctgc cggcccttcc
     1441 tgcggcttgc cagattagat cgccccgtcg ggggatggct cttgctgttt ccttgctggt
     1501 ggggccagct tttggccgaa ggtgcgctcg gtcggacctg ggccgatgcg ttctatcttt
     1561 ggctttttct catcggcgcc ttcgtcatgc gcggcgccgg ctgcacctat aacgacattg
     1621 tcgatcgcaa ctatgacgtg cgtgttgcgc gcaccgcggc ccgcccaatc gcctcgggac
     1681 aggtgagctt ggtcgaggcc cagctgtttt tgggcgtgct gagcctcgtt ggcttgctcg
     1741 ttctcgttca gttcaacctg tttacgatcc tgcttggtgc aagctctttg gtgctcgttg
     1801 cgctttaccc ttttgccaag cgctacaccg actggccaca ggtgatgctc ggcctgacct
     1861 tcaagtgggg ggcactggtc ggctggtcgg ccgttgccgg ctcgctctca gcggctccgc
     1921 ttatgcttta tctcggctgc gtgctctgga cggtcggcta cgacaccatc tatgcccacc
     1981 aggacaagga ggatgacttg gccatcgggc tcagatcaac agccctaaaa ctgggagacg
     2041 cgacccaatc gtggttggtc ttcttctatg cgggcgccgc cgtcgcgtgg gccgcggcgg
     2101 gggtgctggc gggcgcgggg gcgctctatt tcctcgccct cctgctggtt tgcgcgcact
     2161 tcggctggca gatcaccacg ctcgttgtcg cggacgccgc caattgcttg gcgcgttttc
     2221 gctccaatca gctgatcggt tggatgctgt gcctcggcct cgtcttggat ttgctgatga
     2281 aggcgcacac ctgacacgcg agcacgcgcg cttgcggtgc caggcgcggt gtcggcggcg
     2341 tagtgccgcc aacgcccgcg tgctggtata gccttttccc gtcaacccct gagcggccgt
     2401 cttgcgcagc ctagcctcat cagcctccca caaaaggctt ccgaagcgct taagggaaag
     2461 ccttaatttg acgttagcag gggcggtccc ttggccaggc gccgccgccg caggaaggac
     2521 ctcaacttgc caaggaggcg cagcgtcatg ccccccgtca gcaccccgag accggccaag
     2581 cagcttctcc accttgtctt tgggggcgag ctcaagacct tgacggacgt ggaattcaaa
     2641 gacctcgatc ggctcgacgt ggtcggcatc tacccgagct atgcggaagc ctacaaggca
     2701 tggaaggccg ctgcccagcg cagcgtcgat gacgcgcaga tgcgctactt tatcgtgcac
     2761 atccatcgct tgctcgatcc cacccccggc gtcggcgcgg aaccttgagg tacgcggcaa
     2821 tcgcccacag gtcttgcccc cgcttgtggt ggtctcattc tcgctgacct gatatctagc
     2881 tgacgcgggc aaacaattgg gagcaccttg cgcaaaacct cgaacctcgc cggcaggaaa
     2941 gccaaatcgt tggccctcag cgcgcgcgag cgcggactat tgcggcgatt tcttgctgat
     3001 tggatccggc cgcgctggcg ccagattgtc ttggccctcc tgttcacggg gggattggcg
     3061 gcggcaaccg cgggctatcc gctgatcatc aaatcggcct tcgattcgct gatgcagccg
     3121 ggcagcttgg cgctgccttg ggtgctggtt tcggtgatcg gcatcaccgc ttgccgcggg
     3181 ctcttccttt atttgcacca gatcacgacg gctcgcatcg tcacgcggat ggtgacggat
     3241 attcagaaaa cggccttcgc gcatctcatc aatggggact tcgcccgctt cggacgcgag
     3301 actaccggac acctcgtgtc ccggctcacc aatgatctga ccttcattca acaggcggc
//
LOCUS       DASTMT010000312         5507 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_172664, whole genome shotgun sequence.
ACCESSION   DASTMT010000312 DASTMT010000000
VERSION     DASTMT010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 5507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5507
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172664"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..279
                     /locus_tag="VFR00_07310"
     CDS             <1..279
                     /locus_tag="VFR00_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012112877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEU0159103.1"
                     /translation="RRVLWPLALPGIVAGAIFCFILAMNAYATPVLLGGPQFKMMGPL
                     VFGQFRLNNWPFGAAVAFVLMTATLLLTATANVLGQRRYRRSLHGAPV"
     gene            298..1677
                     /locus_tag="VFR00_07315"
     CDS             298..1677
                     /locus_tag="VFR00_07315"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU0159104.1"
                     /translation="MAQAAKKIVICGAGIAGVAAAYYLAVEYGHADVVLVEAADPLSL
                     TSDKSTEGYRNWWPGPDQAMTAFMNRSIDLIEAIARATGNRIAMNRRGYLFATAEPST
                     VARLEQLARTAEQLGTGPVRVHADRSSCYRPPAATDFEAAPTGADIITEPTLIRRHFP
                     QLAPATVAVLHARRAGWLSAQQLGMVRLEGARAKGGVKLLRATVVGVSLAGGRVNAVE
                     IEQGSERQSLPADAFVIAAGPMQKEIAALIGVDLPVFAERHLKISFQDRLGAVPRGSP
                     MLIWLDRQHLPWSEEERAVLAAEPETRWLLQEFAAGVHARPEGGGERGTLLLLFNYEA
                     GATQVVFPLPEAPHYAEIALRGAARMVPALAAYFGAGVRPFIDGGYYVKTRENRPLIG
                     PLPVEGAYITGAFSGFGVMAACAAGDLLARHISAAALPNYAPAFLLERYGEPAYQARL
                     AGWGDGGQL"
     gene            complement(1698..2270)
                     /locus_tag="VFR00_07320"
     CDS             complement(1698..2270)
                     /locus_tag="VFR00_07320"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HEU0159105.1"
                     /translation="MTSIYTTVAYAAWGLILLVWIPGSFMSAKAVRRGRPLLQVFTTS
                     LIALAFFLLFQRRSSGLLGRRLTEVSAPVALFADLVCLGAVAFAIWARLTLGRNWSGA
                     LAAVGENHELIVRGPYRYLRHPIYASFLIAMLATATTIGTLASYLAVPVGLLAFLLRI
                     RIEEALMLSQFPDDYRAYRRQTPALIPGIF"
     gene            2561..3436
                     /locus_tag="VFR00_07325"
     CDS             2561..3436
                     /locus_tag="VFR00_07325"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HEU0159106.1"
                     /translation="MMASYRRIKVQPVAGALGAEIGGIDLGALDDETFAEIKAAWLQH
                     LVVFFRNQSITPEQQLAFAKRFGEIHHHPFMQGMEAYPDILEILKEEGDTKAFGEVWH
                     TDQMFNPKPAKATILYAKETPDAGGDTLFTNLYLAYETLSEPMQLLLKDVRTWNVGDR
                     KRLGRTHEITATRDGRYVGNATMAAKVKDPGDLVTEAIHPLVRTHPETGRKALYLSNH
                     TQTLDGFRPEEARPILAFLASHAVEPEFTCRFRWEVGSLAVWDNRCTQHRALNDYHGK
                     RRRMHRITIAGDAPF"
     gene            3599..4111
                     /locus_tag="VFR00_07330"
     CDS             3599..4111
                     /locus_tag="VFR00_07330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0159107.1"
                     /translation="MRKPQFIVAAGAATGVISMIVGVFGIYRAWPAPEGLATAAQRVT
                     YALQWEAIAALPLLVAVIAVGNNRFLSEAIDPTLNKEDLATQINGRVVENTLQQLVLF
                     VLGTLALSVGLEQEQMGIIPAAVIVFVLARGAFWIGYRLNPLYRAFGMAATGYLNVGL
                     LAFAIAKLFA"
     gene            complement(4848..5336)
                     /locus_tag="VFR00_07335"
     CDS             complement(4848..5336)
                     /locus_tag="VFR00_07335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0159108.1"
                     /translation="MKSRSVVPEILDRLEPHLESLELAWSAQADGKMELLYLHPRRQD
                     QRTSNRARFWAPETLEQHFYSKPDLAGPVNALALFKIIGSWMLDDVADEGMQRRLKQT
                     ADDRSDLRKALAEREALFEALRAENARLKANLDLIEETGLAMRLDAMTLVVVDAIAPD
                     PW"
BASE COUNT          935 a         1775 c         1788 g         1009 t
ORIGIN      
        1 aggcgcgtgc tctggccgct ggccctgccc ggcatcgttg ccggcgccat tttctgcttc
       61 atcctggcca tgaatgccta cgccacaccg gtgctgctcg gcggcccgca attcaagatg
      121 atgggaccat tggtgtttgg acagttccgg ctcaacaatt ggccgttcgg ggcggcggtt
      181 gccttcgtgc tgatgacggc aacgctgctg ctcacggcca ccgccaacgt gcttggccag
      241 cgtcgctatc ggcgcagcct gcacggcgcg ccggtctgac aaggagagcc ccgaagcatg
      301 gcgcaggcgg caaaaaagat cgtcatctgc ggcgccggca tcgccggcgt ggcggcggcc
      361 tactacctag ccgtcgaata tggccacgcc gacgtggtcc tggtcgaagc cgcagacccc
      421 ctgtccttga cctcggacaa gtccaccgag ggctatcgca attggtggcc gggcccggac
      481 caggccatga ccgcctttat gaaccggagc atcgacctaa tcgaagccat tgcgcgtgcc
      541 accggcaacc gcatcgccat gaaccggcgc ggttatctgt ttgccaccgc cgagcccagc
      601 acggtggccc ggctcgaaca gctggcgcgg acggccgagc agctcggcac tggcccggtg
      661 cgcgtgcacg ccgaccgctc cagttgctac cgaccgccgg ccgccaccga cttcgaggcg
      721 gcgccgaccg gcgccgatat tatcaccgag ccgaccctga tccgccgaca ctttccccag
      781 cttgcgccgg caaccgtcgc ggtgctgcac gcccgacgcg ccggctggct cagcgcccag
      841 cagctgggca tggtgaggtt ggagggcgcg cgcgccaagg gcggcgtaaa gctgctgcgg
      901 gcgacggtcg tcggggtcag cctggcgggc gggcgcgtca acgccgtcga gatcgagcag
      961 gggtccgaac gacaatcgct gccggccgac gcgttcgtga tcgcggccgg cccaatgcaa
     1021 aaagagatcg ccgccctgat cggcgtcgac ctgcccgtgt tcgccgagcg ccatcttaaa
     1081 atcagcttcc aggatcggct cggtgccgtg ccgcgtggct cgccgatgct gatttggctc
     1141 gatcggcagc atttgccctg gagcgaagag gagcgcgccg tgctggccgc cgagccagag
     1201 acgcgctggc tgctgcagga gttcgcagcc ggcgtgcacg cgcggcccga gggcggcggc
     1261 gagcgcggca cgcttttgct gctcttcaac tatgaggcgg gcgccacgca agtcgtcttt
     1321 ccgctgccgg aggcgccgca ttatgccgag atcgcgctgc gcggcgccgc ccgcatggtg
     1381 ccggcgctcg ccgcctactt cggcgccggc gtgcgtccgt tcatcgacgg cggctactac
     1441 gtcaagaccc gcgaaaaccg ccctttgatc ggtccattgc cggtcgaggg cgcctatatc
     1501 acgggggcat tttcagggtt cggggtgatg gctgcctgcg ccgcgggcga tctgctggcg
     1561 cggcatatct cggccgcggc gctgccaaac tacgcgccgg ccttcttgct cgaacgctat
     1621 ggcgagcctg cctatcaggc gcgcctcgcc ggctgggggg acggcgggca gctctgagcc
     1681 gcccggaccg tcgcggctca gaaaatgccg ggaatgagcg cgggtgtttg ccgtcggtag
     1741 gcgcgatagt cgtccgggaa ctgcgacagc atcaaagctt cctcgatgcg gatgcgcagc
     1801 aggaaggcca gcagcccgac tggaaccgca aggtagctcg caagcgtgcc gatggtcgtg
     1861 gccgttgcca gcatggcgat cagaaagctg gcatagattg ggtggcgaag gtagcgatag
     1921 ggtccgcgca cgatgagctc gtgattttcg cccaccgcgg ccagcgcgcc cgaccaattg
     1981 cggccaagcg tgagacgcgc ccagatggcg aacgccaccg cgccgaggca aaccagatcg
     2041 gcgaataggg caaccggggc gctgacttcg gtgagccgcc gccccaacag cccgctcgat
     2101 cggcgttgga acaacaagaa gaaggcgagc gcgatgaggc tggtggtgaa cacttgcagg
     2161 agcggccggc ctcggcgcac cgccttggcg ctcataaaag agccggggat ccaaacgagc
     2221 aggatcaggc cccaggcggc gtaggcaacg gtcgtgtaga tcgaggtcat gggcggccaa
     2281 accggtgcgc aggctctcaa tcgaagctgg cgcgcaatcc tgggcgcttc tgcggcgggg
     2341 aggcctgggt ctcggcggtc cccgggggtg ttggccccgg ggcttttggc gttttgcttt
     2401 tgcattggcg cgccggtggg caggttcgcc cttgctcttt tttttcagga gctcgctcga
     2461 ggccgcttgc gccaaggcgc cctctccagc caccggttga cccgaaggcg ggggttttgc
     2521 aagaattcgg ggcgtggcgc gggcggaata tagggggcag atgatggcat cctatcgtcg
     2581 cattaaagtt cagccggttg ccggagcgct tggcgccgaa attggcggca tcgatctcgg
     2641 cgccctcgat gatgagactt tcgcggagat caaggccgcc tggttgcagc atctggtcgt
     2701 ctttttccgc aaccagagca tcaccccgga gcagcagctc gcctttgcca agcgcttcgg
     2761 cgaaatccat caccacccct tcatgcaggg catggaggcc tatcccgaca tcctcgaaat
     2821 cttgaaggag gaaggggaca ccaaggcgtt cggtgaagtc tggcacaccg atcagatgtt
     2881 caaccccaag ccggcgaagg ccaccattct ctacgccaag gagacgccgg acgccggtgg
     2941 cgacacgctg tttaccaatt tgtacctcgc ctacgaaacc ctgtcggagc cgatgcagct
     3001 gttgctgaag gacgtgcgca cctggaacgt cggcgatcgc aagcggctgg gccgcaccca
     3061 tgagattacg gcaacgcgcg atggccgcta tgtcggcaat gcgaccatgg cggccaaggt
     3121 gaaagatccg ggcgatctcg tcaccgaggc gatccatccg ctggttcgca cccaccctga
     3181 gaccggtcgc aaggcgcttt atctcagcaa tcacacgcag acgctcgacg gcttcaggcc
     3241 cgaggaagcc cggccgatcc tggcgtttct ggcgagccac gccgtcgagc cggagttcac
     3301 ctgccgcttc cgctgggagg ttggctcgct cgccgtctgg gacaaccgct gtacgcaaca
     3361 ccgcgcgctc aacgactatc atggcaagcg ccggcgcatg catcgcatca ccatcgccgg
     3421 cgacgcgccc ttctagcggt gcgggcaatc gtccggccgg gtgcggttgc ggcggcagcg
     3481 tcgggcctca cgctgaccgg caccagcttg cgaggtgcca ccgcgtcagc agttgatccg
     3541 agactgagcc ctgcgtgagg agatcgcgcc cgtcacgcag ctgcctggga gaggtcacat
     3601 gcgcaagccg caattcattg tcgccgcggg tgcggccacc ggcgtcatca gcatgattgt
     3661 cggtgtcttc ggcatctacc gcgcctggcc ggcgcccgaa ggcctcgcca cggcggccca
     3721 gcgcgtcacc tacgctttgc aatgggaggc cattgccgca ctccccctgc tcgtcgccgt
     3781 gatcgccgtt ggcaacaacc gctttttgag cgaggccatc gatccgaccc tcaacaagga
     3841 ggacctcgcc acgcaaatca acggtcgcgt ggtcgagaat accttgcagc aactcgtgct
     3901 gtttgtgctg ggcacgctgg cgctcagcgt cggcctcgag caagagcaaa tgggcatcat
     3961 tcccgcggcc gtcatcgtct tcgtgctggc gcgtggcgcc ttttggatcg gctatcggct
     4021 caatccgctt tatcgcgcct tcggcatggc cgccaccggc tacctcaacg tcgggctcct
     4081 cgcttttgcc atcgccaagc tgtttgcttg aggcgcgggc tgaggcgacg gacgtcgttt
     4141 ttggcgcgcg ccgcgtggcc agccgggccg gcgccgtgcc ttgacccctc aaagaggggg
     4201 ctttgagcgc cgggctttgc aacagttgag ttttgctggc ggtgccgggt cgcaaatgtg
     4261 ggcagcctgc tgccggcacc gctccttgct aggcatcgtc cgctcacgat ccgctcgctc
     4321 cggcccgcga gccggtcgct tggttagcat gcgagcggct ggcgtcgtta gctgcagccg
     4381 ctggtgcctc cgcagtaata atactagcta gctcaacgag tgtgtatgta ggcgcttttc
     4441 caagtcggaa tatgccaaac gcttattccc ggcagacttg gacggaataa ggtaagcgac
     4501 tgcaagcgac gtaacatgaa gctgatttgc attgcagctg ctttggtctc tccaaggatc
     4561 aggatcgcca gagccgctcc accaccatca gcggcttgtc cgtagcaccg gcgtgctcgg
     4621 tcgcagggac gttgcgctcc acgcccttgc gtcctgcgcg cgactggcac tgacgggacg
     4681 cctcgacggg tggctgcggg gaatcatgcg aaggcagaag ctagctgccg caggtaggcg
     4741 aagggcagcg gccaatttag gccgcttaga cgccggtccg cccagggggc gttgcaatgc
     4801 gtcggagatg gtgtcgacgg atccaatgac aatcgcccac gcccggttca ccaaggatcg
     4861 ggagcgatgg cgtcgacgac caccaacgtc atggcgtcca gccgcatcgc aagaccagtc
     4921 tcttcgatta gatcgaggtt ggctttcagg cgggcatttt cggcgcgtag agcttcgaac
     4981 aacgcttccc gttctgcgag cgccttgcgt agatcggatc tatcgtccgc tgtttgtttg
     5041 aggcgccgct gcattccttc gtcggcaacg tcatcgagca tccatgatcc gatgatcttg
     5101 aacagtgcca gcgcgttgac cgggccagcc aggtctggct tgctatagaa atgttgctcc
     5161 agcgtctccg gagcccaaaa tcgcgcacga tttgacgtac gttgatcttg ccgtcggggg
     5221 tggaggtaga gtagttccat ttttccgtcg gcttgcgcgg accatgccaa ctccaaggac
     5281 tccaaatgcg gttcgagccg gtccaggatc tccggaacta cggagcgcga tttcatcttt
     5341 tcacccgaag catggtaact cttcctccgg agaggcggga aagcattacg gcattctcct
     5401 ctttcaaacg cgcgttttcc tgtcggagtc ttctgagctc gagctctact tccgtccaac
     5461 cctgcatgat cttcttgact cggccatcag catcggacac gcgggcc
//
LOCUS       DASTMT010000313         3532 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_833948, whole genome shotgun sequence.
ACCESSION   DASTMT010000313 DASTMT010000000
VERSION     DASTMT010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3532)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3532
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_833948"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..659)
                     /locus_tag="VFR00_07340"
     CDS             complement(<1..659)
                     /locus_tag="VFR00_07340"
                     /inference="COORDINATES: protein motif:HMM:NF015413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HEU0159109.1"
                     /translation="MNISARSNRVPAAPPFEVAEAAPKAHILSAHQAVDIAEKLNGLD
                     QPEAVRVLEAMPIAAAIQVFNVRDLRAAAELLELIAVDWAGAILSGLQPDRRAQIFRQ
                     LSDVGRERLQGHLPGPMREALDQLLQYPAQSAGGLMTTEFVAVPADWTVARTLQHIHD
                     VGRNRETVYTVCVLEPTSRRLLKVIGLWRLVVSEPDSKVLEAAQAQAPIAVSAWTDRE
                     EV"
     gene            1108..2103
                     /locus_tag="VFR00_07345"
     CDS             1108..2103
                     /locus_tag="VFR00_07345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008968872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU0159110.1"
                     /translation="MTNLDRRRVLFGAMAASGAALLDPAPPARAGMPAAGTQNVGWYR
                     YKVGSHEITVVTDGVNRFKFPDGFVANKTREEVNEGLASLHLQPGPDMVAVPYTPIVV
                     NTGSKLVVMDTGTGEANLERSKGAAGQFHGNLKAAGIDRNQVDTVIISHFHGDHINGL
                     LTPDSKPSFPNAEILVPAAEWKYFMDDGEMSKQTSERMKGVFAGLRRVFDALNRKVTP
                     YEPDKEVAPGITSVATYGHTPGHNSFIVASGNSKVFVQADVTNIPLFVRNPGWHLFFD
                     QDPVMAEATRRKTYDMLVAEKMLVQGFHYPFPALASIEKSGTGYRETLVPWSPTL"
     gene            complement(2198..>3532)
                     /locus_tag="VFR00_07350"
     CDS             complement(2198..>3532)
                     /locus_tag="VFR00_07350"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HEU0159111.1"
                     /translation="SSAINGMVWVRGQRQDYDHWAQLGNRGWSYQDVLPLFKGIESYR
                     GGDPAVRGRQGPIIVSDADERGRLYDSVFAAAETVGIRPNRDYNGPDQEGVAMTQASI
                     GRGRRMSTARCYLDPARTRRNLSIETNALAEQLLLEGHRCVGVRYSVGGLKREARAAH
                     EVIVSAGAIATPQLLELSGIGQSERLKGLGIEVRHHLAGVGENLRDHWAPRMKWRVGR
                     HGVTFNERARGLRGLGQGLLYIGARRGFLSHPASPMRAFFKTREGLDSPDAMFTLQPF
                     LMTPALKLAREPGLTIITHQLRPESKGSVHITAADAKKPPAIRFNFLAERIDRECLLA
                     SMRLVRRLVAAPALAWLGAEEFAPGPTLASDEELIGFVTRTAETTYHPVGTCKMGSDP
                     MAVVDDQLRVHGLAGLRVADASIMPTLTSGNTNAPSIMIGEKAARMVLAAAT"
BASE COUNT          602 a         1160 c         1157 g          613 t
ORIGIN      
        1 acctcctcgc gatcggtcca cgcggaaacg gcaatgggtg cctgggcctg tgccgcctcg
       61 agcactttgc tgtccggttc gctcacgacc aggcgccata gcccgattac cttcagcagg
      121 cgcctggagg tcggctccag cacgcacacc gtatagacgg tctcgcgatt cctgccgacg
      181 tcgtggatat gctgcagcgt acgcgcgacc gtccagtcgg ccggcaccgc cacaaactcg
      241 gtcgtcatca gtccgcctgc cgattgcgcc gggtattgca gcagctgatc gagggcctcg
      301 cgcatgggcc cgggcaggtg cccctgcagg cgctcacggc ccacatcgct cagctgtcga
      361 aaaatctgcg cccgccgatc cggctgcagg ccgctcagaa tggctccggc ccaatccacg
      421 gcgatcagct cgagcagctc cgccgcggcg cgcagatcgc gcacgttgaa gacttgaatg
      481 gctgcggcaa tcggcatggc ctcaagcacg cgcacggctt cgggctggtc gagcccattg
      541 agcttctcgg cgatgtcgac ggcctggtgg gcagatagaa tgtgcgcctt tggcgcggcc
      601 tcagccacct caaaaggcgg cgctgcgggc acgcggttgc tgcgggcgga aatgttcatg
      661 gcacttgacc tcgggtctcg ccgccggggg cgaccgaggt aagcgcctca ggcgaggacg
      721 attagcaccc gatcaccgcc gacggcctcg tcggttgact gtcgctcggc atgtcttgcc
      781 tatctcctcg cgaacacttg ggggcccagg gggggaccga gggtacccct ttgcgtagat
      841 gcggctggag ttctccatcc ccaacgcctc tcctgtcaat ggggagcacg gacgcagttg
      901 gcaaggggct gggctcgggg gcgggtcgca caaccgcaag cccaggggct tgccccgccg
      961 aaccagcccc cctctcggtc cgatggcgcc ggccggcggg ctggcgacgg tgccgcggcc
     1021 acgtgctaag acaaggccgc acggggtggc ggacgttgtc ttcgcgcatg ccgtgctcag
     1081 ggcctcaact tgcttaggga gaaacgcatg acaaaccttg atcggcgccg tgtgttattt
     1141 ggagccatgg cggcttcagg tgccgccctc ctcgatcccg ccccacccgc gcgtgcgggc
     1201 atgccggcgg cgggcaccca gaatgtgggt tggtatcgtt acaaggtcgg cagccacgag
     1261 atcacggtcg tcaccgatgg cgtcaaccgc ttcaagtttc ccgacggttt cgtcgccaac
     1321 aagacgcgcg aggaggtgaa cgaaggcctg gcctcactcc acctgcagcc gggccccgac
     1381 atggttgcgg ttccctacac gccgatcgtc gtcaacacgg ggagtaaact cgtggtgatg
     1441 gatacgggca cgggcgaggc caacttagag cgcagcaagg gcgccgccgg ccaatttcac
     1501 ggcaatctga aggccgccgg aatcgaccgc aatcaggtcg acaccgtcat tatctcccac
     1561 tttcacggcg atcatatcaa cggcctcctg acgccggaca gcaagccgtc cttcccgaat
     1621 gccgaaatcc tggttcccgc cgcggaatgg aagtacttca tggatgacgg cgagatgagc
     1681 aagcagacaa gcgagcgtat gaaaggcgtg tttgccgggc tgcgccgcgt gttcgatgcg
     1741 ctgaaccgca aggttacgcc ctacgagccg gacaaggagg ttgcgccggg catcacctcg
     1801 gttgcaacct acggccacac ccccgggcac aactccttca tcgttgcgtc aggcaatagc
     1861 aaggtgttcg tccaggccga cgtcaccaac attcctctgt tcgtacgcaa tcctgggtgg
     1921 caccttttct tcgaccagga tcccgtcatg gccgaagcca cgcgtcgcaa gacctatgac
     1981 atgctggtgg cggaaaaaat gctggtgcag ggcttccact atcccttccc cgcgctggcc
     2041 tccatcgaaa agagcggcac gggataccgt gagacgctcg tgccctggag cccgacgctc
     2101 tgacgccggc gtccgttggc aggtcggccc cgtgccggcc tgccgcaagg ccgcatcatc
     2161 accatagccg cggggctgtg ccgggctgtg gtctcctcta ggtcgcggcc gcaagcacca
     2221 tgcgtgcggc tttctccccg atcatgatcg agggtgcgtt ggtgttgccc gaggtcaggg
     2281 tcggcatgat ggaggcgtcg gccacgcgca ggccggcaag gccgtgcacg cgcaattgat
     2341 catccaccac cgccatggga tcgctgccca ttttgcaggt gccgacggga tgataggtgg
     2401 tctcggccgt tcgggtgacg aagccgatga gctcctcatc ggacgcgagc gtgggacccg
     2461 gcgcgaattc ttcggcgccg agccaggcga gcgccggcgc ggcgaccagc cgccgcacca
     2521 aacgcatgct ggccagcagg cactcacggt caatgcgttc agccaggaaa ttgaagcgga
     2581 tggccggggg ttttttggca tcagcggccg tgatgtggac gctgcctttg ctctcgggcc
     2641 tcagctggtg cgtgatgatg gtgaggccgg gttccctggc gagcttcagc gccggcgtca
     2701 tcaaaaaagg ctgcagggtg aacatggcat cggggctgtc gagaccctcc cgcgtcttga
     2761 agaaggcgcg catgggcgag gctggatgac tgagaaagcc tctgcgggcg ccgatataga
     2821 ggagcccctg gcccaggccg cgcagcccgc gtgcccgctc gttgaaggtc acgccatgcc
     2881 ggccgacgcg ccacttcatg cgcggcgccc agtggtcccg cagattctcg ccgacgcccg
     2941 ccaagtggtg cctgacctcg atgccgagcc ccttgaggcg ctcgctttgg ccgataccgg
     3001 agagctccaa taactggggc gtggcgatcg ccccggcgga aacgatcacc tcgtgggcgg
     3061 cacgggcctc gcgcttgagg ccgccgacgc tgtagcgcac cccgacgcag cgatgaccct
     3121 cgagcaatag ctgctcggcc aaggcattgg tctcgatgct caggttgcga cgcgtgcgtg
     3181 ccggatcgag gtagcagcgt gccgtgctca tgcgccgacc gcggccgatg gaggcctgcg
     3241 tcatggcgac gccttcctga tctgggccgt tgtaatcgcg gttgggcctg attccgacgg
     3301 tctcggcggc agcaaagacg ctgtcgtaga gccggccgcg ttcatcggcg tccgaaacga
     3361 tgatcgggcc ctgccggccg cggacggcag gatcgccgcc gcgatagctt tcgataccct
     3421 tgaagagcgg cagcacgtcc tggtagctcc agccccgatt tccaagttgt gcccagtgat
     3481 cgtagtcctg tcgctggccg cgcacccaaa ccatgccgtt gatcgcactt ga
//
LOCUS       DASTMT010000314         6049 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_6325126, whole genome shotgun sequence.
ACCESSION   DASTMT010000314 DASTMT010000000
VERSION     DASTMT010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 6049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6049
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6325126"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(324..1127)
                     /locus_tag="VFR00_07355"
     CDS             complement(324..1127)
                     /locus_tag="VFR00_07355"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0159112.1"
                     /translation="MAALLIALLPLAASPQSKPGKQEEALMSQEVKAELASTGVLRAG
                     INLSNFLLVTGKTDAGDPVGVAPDMASEIARRLGVPVRFVTYKTPGELADAADTGAWD
                     IGLIGAEPQRAEKISFTPAYVEIEATYLVPAGSRLQAIADVDRPGVRIAVTGRSAYGL
                     WLDRNIKQAELVKSGTLDSAYEQFVAEKLDALAGLRPRLLSDVEKLPGARILDGQFTA
                     VQQAVGTARRNGAAAEYLRNFVAEAKASGFVGRLIEKHKVRGLSVAPPA"
     gene            complement(1314..2861)
                     /locus_tag="VFR00_07360"
     CDS             complement(1314..2861)
                     /locus_tag="VFR00_07360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018325375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU0159113.1"
                     /translation="MASEHGQPTGPDLAQGIASAALADGGMLAGRVGDKAVLLARCGK
                     DIFAVDAACTHYGAPLADGLLDGTTVRCPWHHACFDLKTGEALHAPAFSPLGCWEVEE
                     GAGRIVVRARRTLAAGSERAPERATEPRRIVIVGGGAAGFAAADMLRRLKYDGAISIL
                     SADAAAPVDRPNLSKDYLAGNAPQDWLPLRPDPYYAEQGIHLKLNANVAAIDVGAQQV
                     VLAGGARVAYDRLLLATGAEPLRPPIPGAQGRHVYVLRSLADCQNILTAAAAAKRVVV
                     LGASFIGLEVAASLRARGLEVHVVAPEARPMQRLLGPQLGALVQSLHEEHGIHFHLEE
                     LASAIAAEHVELKGGARLAADFVVAGVGVRPRLGLAEAAGLSIDRGVLVDSYLETSAA
                     GIFAAGDIARWPDPAGSGSLRVEHWVVAQRQGQTAARNMLGLRQKFQAVPFFWSQHYD
                     LSISYVGHAAEWDDIAIDGDVAARDCVLRYRRNGRVLAVASINRDLASLEAELALERA
                     LEAASPS"
     gene            3038..3718
                     /gene="folE"
                     /locus_tag="VFR00_07365"
     CDS             3038..3718
                     /gene="folE"
                     /locus_tag="VFR00_07365"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HEU0159114.1"
                     /translation="MNTLLRIDPEAPGVTVAPLSTGRGGTVERPTRAEAEQAVRTLIR
                     WAGDDPTREGLLETPARVVRAYQQWFAGYQQDPAQHLFRTFAEVAGYDEMVVMRDISF
                     QSHCEHHMAPITGLVHIGYLPRNKVVGISKLVRVVQVFAKRLQTQEKLTAQIADTLYG
                     VLAPQGVAVVVEGRHGCMTSRGVQQHSATMLTSRMLGAFRDRPETRQEFLAAIDLDGA
                     GQPARARL"
     gene            3948..4760
                     /locus_tag="VFR00_07370"
     CDS             3948..4760
                     /locus_tag="VFR00_07370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008834126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipo-like protein"
                     /protein_id="HEU0159115.1"
                     /translation="MTAFDSFLDWFGRKLGDRLRREVPGQEPFVPSNPVALRRVLRPA
                     DVLLIAGGSKLSTAIKYLTQSTWSHAAIYVGDWFELPSPDGEAHVLIEVNLGEGCISA
                     PLSKYDRFHTRICRPIGLTEADREEVLAYMVSRLGTQYDMRNIIDLLRYLLPEPPIPA
                     RWRRRMIALGSGEPTRAICSTLIAEAFGRVRYPILPRVERRASAERGVTRFQRSEILH
                     IRHHSLYAPADFDLSPYFKIVKPTIEEGFNYKGLTWAEDEVVARPAAKRKAN"
     gene            complement(4809..5303)
                     /locus_tag="VFR00_07375"
     CDS             complement(4809..5303)
                     /locus_tag="VFR00_07375"
                     /inference="COORDINATES: protein motif:HMM:NF017950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF992 domain-containing protein"
                     /protein_id="HEU0159116.1"
                     /translation="MRGAPLLGLIALLGSGTFAVAQELAEIGNLTCSLAEQSGSPDTE
                     AGIGQARDMLCTFKPAARGAEETYTGTLQSAGDERPFDKGVLMWTVRGPAATEIQPGL
                     LQQSFTVDAATAANQIAPLVGEKSPIVLQAAKKEMQQAPDSDSAKPTTVIITIVLKLK
                     TSPA"
     gene            complement(5436..>6049)
                     /locus_tag="VFR00_07380"
     CDS             complement(5436..>6049)
                     /locus_tag="VFR00_07380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013419983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEU0159117.1"
                     /translation="ELQVAEDHFDLAIVDWMLPGLSGIELCRRLRRREDTRGVPILML
                     TARGEEDDRVRGLGIGADDYVVKPFSLPELLARIKALLRRASPNRVADALQGGDIVLD
                     RVAHRVTRGGREVRLGPTEYKLLAYFMENAGRVLSRQQLLDGVWGRDAFIDGRTVDVH
                     VGRLRKALMQEGENDPVRTVRGAGYAFGADSRSVQSVAASKPG"
BASE COUNT         1002 a         2099 c         1982 g          966 t
ORIGIN      
        1 cggcaacgca cgcgaactca ggtcccctgc ctgtcccccg ggggcgggtc ccacccgacc
       61 ctcgaggcct attgttaagc gtttgatgta aatcgcaatc tgggcgggat tgggcaggcg
      121 gcgaggcgtt tcaggggcca cctcggcgct tcggcgcggg aaatcgtccc agcgccgccc
      181 ccgcgccaag atctcccggc caagcgccac agccgcgccg ccgcggcaag gcgggtcggc
      241 aagccgcctt cggccgcgcc cagccgcaag tcattcgtgg gacaggacct tgccggccaa
      301 gcccgcagca acggccggca cgcttaggcc gggggcgcca cggacaggcc gcgcaccttg
      361 tgtttttcaa tcaggcggcc aacgaacccg gaagcctttg cctccgccac gaaattgcgc
      421 aggtattcgg ccgccgcgcc attgcgccgc gccgtcccca cggcttgctg tacggccgtg
      481 aattggccat cgagaatgcg cgcaccgggg agtttttcca cgtcggacaa caggcgcgga
      541 cgcagtcccg ccagggcgtc gagcttttcg gcgacgaact gctcgtaggc actgtcgagg
      601 gttcccgact tcaccaattc cgcctgtttg atgttgcgat cgagccaaag gccataggcg
      661 ctgcggcccg tgacggcgat ccgcaccccg gggcggtcca catcggcgat cgcttgcagc
      721 cgcgagccgg cggggaccag ataggtcgcc tcgatttcca cataagccgg ggtgaaactg
      781 atcttttccg cgcgctgcgg ctccgcaccg atcaagccga tatcccaggc tcccgtatcc
      841 gccgcgtcgg ccaactcgcc cggcgtctta taggtcacaa aacgcaccgg caccccaagg
      901 cgcctggcga tttcgcttgc catatcgggt gccacaccga cgggatcgcc ggcgtcggtc
      961 ttgcccgtga ccagcaggaa attcgacaga ttgatgccgg cgcgcagcac gcccgtcgac
     1021 gccagctcgg ctttgacttc ttgcgacata agcgcttcct cctgcttgcc cggtttggat
     1081 tgtggcgagg ccgccaacgg cagcagcgcg atgagaagag ctgccatgca gccaatccac
     1141 ccgagcatgc taccacctcg ccaccgtcga gccggcactt tagccgctgg cgctcgacaa
     1201 aggccagcgg cctcgatggc cgctgccggg cgcgggggca atggctgcaa gacccgttgc
     1261 ctgatgccgg cagcgagaac ggcggcttgc gtccttacgg cggcttgcgt cctttaagag
     1321 gggctcgcag cctcgagcgc gcgctccaac gccagctccg cttcgaggct tgccaggtca
     1381 cggttgatcg aggccacggc tagaacgcgc ccgttccgcc gatagcgcag cacgcaatcc
     1441 ctggcggcga cgtccccgtc gatggcgatg tcatcccact ccgccgcgtg ccccacgtag
     1501 ctgatcgaaa gatcatagtg ctggctccag aagaacggca cagcctggaa tttttgcctc
     1561 aggcccagca tattgcgggc cgcggtctgg ccttgccgtt gcgccaccac ccaatgctcc
     1621 acccgcaagc tgccgctgcc ggcaggatcg ggccagcggg cgatgtcgcc ggcggcaaag
     1681 atgcctgcgg cgctggtctc cagatagctg tccacgagca cgccccgatc gatgctgagg
     1741 cccgccgcct ccgccagccc gaggcgcggc cgcacgccga cgccggcaac gacgaaatca
     1801 gccgccagcc ttgcgccgcc cttgagttcg acgtgctcag ccgcaatggc gctcgcgagc
     1861 tcttcgaggt ggaagtgaat gccgtgttcc tcatgcaggg attgcaccaa ggccccgagc
     1921 tgcggcccaa gcaggcgctg catgggccgc gcctccggtg ccaccacgtg cacctcgagg
     1981 ccacgggcgc gcaaggaggc tgccacctcg agcccgataa agctggcgcc caacaccacg
     2041 acccgcttgg ccgccgcggc cgcggttagg atgttctggc aatcggcaag cgaacgcagc
     2101 acataaacgt gccggccctg cgcgccgggg atcggcggac gcaagggctc cgcgcccgtc
     2161 gccagcagca agcggtcgta ggccacgcgg gcgccgcccg ccagcaccac ctgctgggcg
     2221 ccaacgtcga tggcggccac attggcgttc agcttgagat gaatgccttg ctcggcgtaa
     2281 tagggatcgg ggcgcaacgg gagccaatcc tgcggggcgt tgccggccag atagtccttg
     2341 gacaggttgg gtcggtccac gggcgcggcg gcatcggcgc tgaggatgct gatggcgccg
     2401 tcgtatttga ggcggcgcag catgtcggcg gcggcaaagc ccgccgcacc gccgccaacg
     2461 atcacaatgc gccgcggctc ggtggcgcgc tcgggcgcgc gctccgagcc cgctgcaagc
     2521 gtccgcctgg cgcgcacgac gatgcgaccg gcgccttctt cgacctccca gcaaccgagc
     2581 ggcgagaacg ccggagcgtg cagcgcctcg ccggttttga gatcgaaaca ggcatgatgc
     2641 cacgggcagc gcaccgtggt gccgtccagc agtccgtccg ccaggggagc tccatagtgg
     2701 gtgcaggccg catcaacggc aaagatgtcc ttgccacagc gcgccaggag aacggccttg
     2761 tcgccaacgc gtcccgcgag catccccccg tcggcgagcg ccgcgctggc gatgccttga
     2821 gccaggtcgg ggcccgtcgg ctgcccgtgc tctgatgcca tagctgacct cctcgtgcgg
     2881 ctaccaccct ctttgcccct cggggctctg cgatagatgc gacagggcag caaaggtttc
     2941 cggggctgcc gagggcgcgc cgcgggaacc aatcggccaa gcccacatcc aatgcccgtg
     3001 aaacctgaaa aaagtgccga gccgccgagg agacagcatg aacacgttgc tcagaataga
     3061 tccggaagcg ccgggcgtca ccgttgcgcc cctcagcacc ggcagaggcg gtacggtgga
     3121 gcgtcccacc cgcgcggagg ccgagcaagc cgtgcggacg ctcattcgct gggccggtga
     3181 cgaccccacg cgcgaggggt tgctggaaac gccggcacgg gtggtgcgcg cctatcagca
     3241 gtggttcgcc ggctatcagc aggatcccgc gcagcatctc ttccgcacct ttgcggaggt
     3301 cgccggctat gacgaaatgg tggtgatgcg cgacatctcg tttcagtcgc attgcgagca
     3361 ccacatggcc cccatcacgg gtctggtgca catcggctac ctgccccgca acaaggtggt
     3421 cggcatctcc aagctggtcc gcgtcgtgca ggtcttcgcc aagcgcctgc agacccagga
     3481 aaagctgacg gcgcaaattg ccgacacgct ctatggcgta ctggcgccgc aaggcgtcgc
     3541 cgtggtggtt gagggcaggc acggctgcat gacgagccgc ggggtgcagc agcacagcgc
     3601 aaccatgctg acgagccgga tgctcggcgc ctttcgcgac cggccggaaa cccgccagga
     3661 attcctcgcc gccatcgacc tcgacggggc cggccagccg gcgcgcgcca ggctttgagg
     3721 ccgccgcccc gggctggctt gagcgccgcc agcggcaagg gctgagcgcg cgcgatcacc
     3781 tcgccttgcg cgcgcaaaaa agcccgccag gctggccctg gggcgatggc agcattgcga
     3841 tcttgggttt gccgatgcgg catcgacatc atcacgcccc agtccctgcg ccaagtgatg
     3901 ggaatcggcc gcaggcgggg cccgatcggc cgtgcgatac ttgaaccatg acggctttcg
     3961 attcgtttct cgactggttc ggccgcaagc tcggcgaccg gctgcgccgc gaggttcccg
     4021 ggcaggaacc gttcgtgccg tcaaatcccg tggcgctgcg gcgcgtgctg cgcccagccg
     4081 acgtgctgtt gatcgccggg ggcagcaagc tctccactgc catcaagtat ttgacccaat
     4141 ccacctggtc gcacgcggcc atttatgtgg gcgactggtt cgagctgccc agcccagatg
     4201 gcgaggcgca cgtgctgatc gaggtcaatc tgggcgaggg ctgcatttcg gcgccgctct
     4261 ccaaatacga ccgctttcac acgcgcatct gccgccccat cggtctcacc gaagccgacc
     4321 gcgaggaggt gttggcctat atggtgtccc ggctcgggac ccaatacgac atgcgcaaca
     4381 tcatcgatct tttgcgctac cttctccccg aaccgccaat tcccgcccgc tggcgccgcc
     4441 gcatgatcgc acttggctcc ggcgaaccta cgcgcgccat ttgctcaacc ctgatcgcgg
     4501 aggcgttcgg ccgggtgcgc tatccgatcc tgccgcgcgt ggaacgccgg gcgagcgcgg
     4561 agcggggcgt gacgcgcttt cagcgcagcg aaatcctgca catccgccac cacagcctct
     4621 acgcccccgc ggacttcgac ttgtcgcctt acttcaagat cgtcaaaccc acgatcgagg
     4681 aaggcttcaa ctataagggc ctgacctggg ccgaggacga ggtggtggcg cgtcccgcgg
     4741 ccaaacgcaa ggcaaactaa cgacgtccgg ccgaagcggc tgttgcggct ctcgccagtc
     4801 ggggctgctc aggcggggct cgtcttgagc ttgagcacga tggtaatgat cacggtggtc
     4861 ggtttggccg agtcgctgtc gggcgcctgc tgcatttcct ttttcgccgc ctgcagcacg
     4921 atcggcgatt tttcgcccac caaaggggcg atttggtttg ccgcggtcgc cgcatccacc
     4981 gtgaacgact gctgcaggag cccgggttga atctcggtgg cagccggccc cctcaccgtc
     5041 cacatcagca cacccttgtc gaaggggcgt tcgtcgccgg cgctttgcaa ggtccccgtg
     5101 taggtttcct cggcgccgcg cgccgccggc ttgaaggtgc acagcatgtc gcgggcctgc
     5161 ccgatcccgg cttcggtgtc gggcgagccg ctctgttcgg ccaggctgca ggtgagattg
     5221 ccgatttcgg ccagttcctg ggcaacggcg aaagtgccgc tgccgagcag ggcgatgaga
     5281 ccgagcaatg gcgcgccgcg catgacctga gatctcctgt tgactgctgc cacaacgccc
     5341 ggcgcagcca gaggttcaag ccaaaggccg ccgcatcccg gaaaattgat cagtgatttt
     5401 ttttaaaaat gccgcgagcg gcggcgggac tggtgctagc cgggcttgct tgcggcgacc
     5461 gactggacac tgcggctgtc ggcgccgaag gcgtagccgg caccgcgcac ggtgcggacc
     5521 ggatcgttct cgccttcctg catcagcgcc ttgcgcaggc ggccgacgtg gacgtccacc
     5581 gtgcgcccgt cgataaaggc gtcgcgaccc cagacgccgt cgagcagctg ctggcgcgat
     5641 aaaacgcggc cggcattctc catgaaatag gcgagcagct tgtattcggt cgggccgagg
     5701 cgcacctcgc gcccgccgcg ggtgacgcgg tgcgccaccc ggtcgagcac gatgtcgccg
     5761 ccctgcagcg cgtcggcgac gcggttgggg gaggcccgcc gcagcagcgc cttgatgcgc
     5821 gcgagcagct ccggcagcga gaagggtttg accacgtagt cgtcggcacc gatgccgagc
     5881 ccccgcacgc ggtcgtcctc ctcgccacgg gcagtcagca tcaggatcgg cacgccgcgc
     5941 gtatcctccc ggcgccgcag gcggcgacag agctcgatgc cggacagccc gggcaacatc
     6001 cagtcgacga tcgcgagatc gaagtggtcc tcggcgacct gcagctccg
//
LOCUS       DASTMT010000315         4239 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_175554, whole genome shotgun sequence.
ACCESSION   DASTMT010000315 DASTMT010000000
VERSION     DASTMT010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4239)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4239)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4239
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_175554"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..192)
                     /locus_tag="VFR00_07385"
     CDS             complement(<1..192)
                     /locus_tag="VFR00_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008963667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0159118.1"
                     /translation="MIDQRDSLAREVDEELRREQLLKLWEKYGTYVVAAVVLIVVGVG
                     GVKYYQYRTTVAAEAAGARL"
     gene            698..1687
                     /locus_tag="VFR00_07390"
     CDS             698..1687
                     /locus_tag="VFR00_07390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0159119.1"
                     /translation="MVSDASSGDGRRADLERDKVPVRSADGVQARSTADLTHELAQLL
                     AATIAQRQLNGGLPATAPPPLNLAGLSANQRILEPTFKDGLSALAYKTPRGTSLVPSV
                     DLSADDEPMPIPSTWREPAPTDDRWLRQQMGATLLGLCAGLLVVVPIVLWLSGWLGEA
                     AQKSRPQRSAEPSLQPIEPRAGEVKTVKVQVRPVDRAAQTAAQYVTGSIDARIPAEPV
                     RVIEPASLPVAKAPEPATRPEPVRQADDRLAEAKQRIESGDVSGARDILTAAEGSAPG
                     PISFALAETYDPNMLAAWGTRGAIADVARARALYHKSLGFGMARAQARLEALQ"
     gene            complement(1847..2161)
                     /locus_tag="VFR00_07395"
     CDS             complement(1847..2161)
                     /locus_tag="VFR00_07395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0159120.1"
                     /translation="MKIRHFLLGATAAIGLGVAALSAEAAPAVATAGDLTAAVRDASN
                     VDQVYWARRCYRHRGYLHCRRHWVDYGYAPYYSYDPYPYYYYRPSFGFFFGGHRHHHR
                     HW"
     gene            complement(2361..2972)
                     /locus_tag="VFR00_07400"
     CDS             complement(2361..2972)
                     /locus_tag="VFR00_07400"
                     /inference="COORDINATES: protein motif:HMM:NF018984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABATE domain-containing protein"
                     /protein_id="HEU0159121.1"
                     /translation="MTTAGARARLLAAGPLGLCLDYANTRCWRGLAQPKETLQRPADL
                     LAWCRRAGLQPVPARWRAPAAAGVFAEAICLRELIYSVFSALATGSSIAAGDLSALNA
                     AIAQAAPRAHIAPFGAGYAWQVDVRIGAGVVSASHLLSPVIWSAADLLVAARAGAVRQ
                     CAEATCRWLFLDQSRNAARRWCDMGSCGNRAKARRHYRKHRAA"
     gene            3113..3979
                     /locus_tag="VFR00_07405"
     CDS             3113..3979
                     /locus_tag="VFR00_07405"
                     /inference="COORDINATES: protein motif:HMM:NF014376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEU0159122.1"
                     /translation="MARRGDPLAVALTHKASHGAAGGAQATLLAAGQGWRVFDIVCTC
                     GPRDRPFEERHESASVSLVMSGSFVCRSRSGTSLLSPGSWLLLSSREPIECSHHHGEG
                     DRCLSFQFEPDLFALLAHGAAGAPLPMARQSLPPLRRHAALTARAMLAVERTAALEEV
                     AFDAAGSIIAAASAAAPKQPSSRQQAAVTRTVRRLSAAPEGRHSLSDLARQANMSPYH
                     FLRTFKLVTGVTPHQWLLRARLRAAAQRLAGGRTPITAIALDVGFDDLSNFIRSFQAE
                     FGRSPRQYRAAA"
BASE COUNT          728 a         1430 c         1418 g          663 t
ORIGIN      
        1 cagccgggca cccgccgcct cggccgccac cgtggtgcga tactgataat acttgacgcc
       61 gccgacgccc acgacaatga gcacgacggc ggccacgacg taggtgccgt acttttccca
      121 gagcttcaaa agctgctcgc gccgcaactc ttcgtcgact tcgcgtgcga ggctatctct
      181 ctggtcgatc atgcttttcc acagtgctgg caggtctcaa aggaaggcgt gccagccatc
      241 aatagcgcgg cgccggacgg gtcggaagag ctttaggacg cgccaagccg atggggtcgc
      301 tatttttttg acttttttgc ggctcagcgg gcgcgcacgc ccgggcgacg ggctcagcga
      361 gcaggcgagc gatggacaat tcggcgcccg ccataatttg tgccatattc caccggtcta
      421 gttgcgcgag cttgaccgcg aaaacagctt ctggcgccca accgcttgcg gcggccccgc
      481 tgttaaccgg tcggcgatgc aaggcgcggg atgacttgac ggcgacgggg cgtccgatca
      541 agggctttag ggaggaccgc aaggcgcgca ggctcactct ttaggaggcg tggtgtctgg
      601 cgcccgaggg cttggctcag gacgaagccc atgacggttc atcgaccggg ccttagacgg
      661 cacggtcacg cgtgagtagc gtgtgagggg gtttgcgatg gtgagtgatg cgtcctcggg
      721 tgatggtcga cgcgcggact tggagcgcga taaggtcccg gtgcgaagcg cggatggcgt
      781 acaggcgcgc agcaccgccg acctcacgca tgaattagcg caactgctgg ccgccaccat
      841 cgcccagcga cagctcaatg gcggcttgcc ggcaaccgcg ccgccgccgc tcaatttggc
      901 gggcctcagc gcgaaccaac gcattttgga gccgaccttc aaggatgggc tgtcggcgct
      961 tgcctacaag acaccgcgcg gcacaagttt ggtgccatcg gtcgacctca gcgccgatga
     1021 cgagccgatg ccgatcccct ccacctggcg cgaacccgcg ccaaccgacg accgctggct
     1081 gcgccaacag atgggggcaa ccctcttggg cttgtgtgcg ggcttgctgg tggtggtgcc
     1141 gatcgtgctc tggctcagcg gttggctggg cgaggccgcc cagaagtcgc ggccgcagcg
     1201 cagcgccgaa ccgtccttgc agccgatcga gcctcgggca ggcgaagtga agacggtcaa
     1261 ggtgcaagtg cgcccggtcg accgcgccgc gcagacggcc gcgcaatacg taacgggtag
     1321 catcgacgcg cggatccctg ccgagcccgt gcgtgtcatc gagccggcct ccctgccggt
     1381 ggcaaaggca cccgaaccgg cgacgcggcc cgagccggta cgccaggccg atgaccgact
     1441 ggcggaggcc aaacagcgca tcgaatcggg cgacgtgtct ggcgcgcgcg acattctcac
     1501 ggcggcagag ggcagtgcgc cgggaccgat ctccttcgcc ctggcggaga cctacgatcc
     1561 caatatgctg gcggcgtggg ggacgcgcgg tgccatcgcc gacgtggcga gggccagggc
     1621 gctctaccat aaatcgttgg gcttcggcat ggcgcgggcg caagcacgct tggaagcgct
     1681 gcagtagcag ggctcaggcg tgacgcagcg ggtgcttggc gcgccggggc gttgcctgtt
     1741 cgcgccttac ctgcggcgcg tgccagacgc cgctttggcg gacagagccc cggtgcgtcg
     1801 cgcccgccgg acgagcaacg gcgcccaggg cccaggccgc accggcctac cagtgccggt
     1861 gatgatggcg atgaccgcca aagaagaacc cgaacgaggg gcgataataa tagtagggat
     1921 aaggatcgta ggagtagtag ggcgcatagc cgtagtcgac ccagtggcgc cggcaatgca
     1981 aatacccgcg atgccgatag cagcggcggg cccagtagac ctggtcgacg tttgaggcgt
     2041 cacgcacggc cgcggtcaga tcgccggccg tcgcaacggc gggcgcagcc tcggcagaca
     2101 gggcggccac tcccaaaccg atggcagcag ttgcgcccaa taagaaatgc ctgattttca
     2161 tcacggcctc catggtgtga gcattcccac agctcttttc ccggcgggaa acacctaatt
     2221 cccaacaggt tccctgaatg gccaatgaac gtcgatgctc ggcagcttgt tccaggccat
     2281 agcaccgtgc gctgcgcaag gctcgccgtc gcgcacggca cgtgcgcacg cccgatccgg
     2341 gcgtcgcatg gtcttagcgc ttaggcggcg cgatgcttcc ggtaatggcg gcgcgccttg
     2401 gcgcggttgc cacacgagcc catgtcgcac cagcgccgcg cggcatttcg gctctgatcg
     2461 agaaaaagcc atcgacacgt agcctccgcg cattggcgca ccgcgccggc tcgcgcggca
     2521 acgagaaggt cggcggccga ccaaatgacg ggggagagaa ggtgcgatgc cgagacgacg
     2581 ccggcgccga tgcgcacgtc gacttgccaa gcatagccgg ccccgaaggg ggcaatatgc
     2641 gcacgcggcg cggcctgcgc aatcgcagca ttgagcgccg acaggtcgcc ggcggcaatg
     2701 ctgctgcccg tggcgagcgc gctaaacacc gaatagatca gctcgcgcaa acagatggcc
     2761 tcggcgaata cgcccgctgc cgccggcgcg cgccagcgcg cgggcaccgg ctgcaggccg
     2821 gcccggcggc accaagccag caggtccgcc gggcgctgca atgtttcctt tggttgcgcc
     2881 aaaccgcgcc agcagcgcgt gttggcatag tcgagacaga gacctagcgg ccccgccgcc
     2941 agcagccgcg cgcgtgcgcc cgcagtcgtc atcactgcgc gccccattgc tcctggcacg
     3001 gcctggtggg tgccgcgcgc tcacctctaa ccagcatagc agttgacagg tttagcagcg
     3061 cggcctaccg tctacaacca cctaggtagt tagaaacgac ggaggcctcg agttggctcg
     3121 gcgcggcgac ccgctggcgg tggcgctcac ccacaaggcg agccacggcg ccgcaggggg
     3181 tgcgcaggca acgctgttgg cggcgggcca aggctggcgc gtgttcgaca tcgtttgcac
     3241 ctgcgggccg cgcgatcggc cgttcgagga gcggcacgaa agcgcctcgg tctcccttgt
     3301 catgtccggc agcttcgtct gccgtagccg cagcggcacc tcgctgttgt cgccaggttc
     3361 ctggcttctc ctcagcagcc gcgaacccat cgaatgctcg caccatcacg gcgagggcga
     3421 ccgctgtctc tcgtttcagt tcgagcccga cctgttcgcg cttctggcgc acggcgctgc
     3481 cggcgcgcct ttgcccatgg cgcggcaaag cctaccgccc ctgcgcaggc acgccgccct
     3541 gacggcgcga gccatgctgg cggtcgagcg cacggcggcg ctcgaggagg tcgccttcga
     3601 cgctgcgggc agcatcatcg ccgccgccag cgctgcggct ccgaagcaac cttcgagccg
     3661 gcagcaagcc gccgtcacgc gcacggtcag gcggctcagc gccgcgccag agggccgcca
     3721 cagcctcagc gatcttgcgc gtcaggcaaa catgagcccc taccacttcc tgcgcacctt
     3781 caagctcgtc acgggcgtca cgccgcatca atggctgctg cgcgcacgcc tgcgggctgc
     3841 cgcccaacgg cttgccggcg gccgcactcc catcaccgcg atcgcactcg atgtcggttt
     3901 cgatgacctt tccaacttca tccgcagctt ccaggccgag tttggccgct cgccacggca
     3961 atatcgcgcc gccgcctgag gcggggcgaa gaagcgcaat tttcgccaag tctcaacccg
     4021 ctcccacggg gcaaggtggc tgcggttcag acgcatggga gatgcaagca tgatcaatca
     4081 cgtttcgatc ggcgtgcgcg acattccgcg gtcaaaacgc ttctacgatg cagccttagc
     4141 cccgctcggc tacacctgcc tcagtcagtc gacggatgga ttgggctatg gcggcgatgc
     4201 cgtggcccta tggatcgggg cgaccgatcg acccgtgcc
//
LOCUS       DASTMT010000316         2609 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_834633, whole genome shotgun sequence.
ACCESSION   DASTMT010000316 DASTMT010000000
VERSION     DASTMT010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2609
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_834633"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1571)
                     /locus_tag="VFR00_07410"
     CDS             complement(<1..1571)
                     /locus_tag="VFR00_07410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0159123.1"
                     /translation="MAVATTAAAVALLMIGAWAGAGPSPRDWLGDPDDALRLVSARAL
                     LEGAPWFDPTLARVGAPEPLASHWSRLVDLPLAALIGALRSLLGQELGELLTRLAWPT
                     ALFATLCAVVGREAYRVGGAWAAAAAASLAVTSIGATAQFWPGRIDHHNVQILCAVAG
                     LLFLVRSIEDRRLGWWAGGLIGLGLAVGYEAIALVAPALALAVLMTLFHPARRLSILP
                     AAAATTLTLMAACLLTLAPAAWFKINCDALSPNIVVLAASGTLGLWAAQRFAARLGTR
                     LAILGTAAAAGVGIFAVLEPACLAGPFGQVDPALNSIWLDQVLETKSLLWFARREPAF
                     ALGQFVFLLAGAGAQIALWRRRRDAAATLATAIVLLAVALGCWQMKLVAYASWLAVLP
                     LARFCAALPASAQFSSTLIRIAAIVLASQATLEGGIRLAGAALPVTPRPGAAAVAAAD
                     PRRPCFYTSSLRMLAAQRPGLVAADLDLGPYIVALTPHRVVAAPYHRLHQGILANHAI
                     LETSRAQAEATMRALG"
     gene            1904..>2609
                     /locus_tag="VFR00_07415"
     CDS             1904..>2609
                     /locus_tag="VFR00_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010682258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU0159124.1"
                     /translation="MSQSDVFVIGAGPAGLTAAYLLTKQGVATTVIERDPTYVGGISR
                     TASYKNFLFDIGGHRFFSKSQEVVALWKEILPDGFIERPRLSRIYYNGTYFSYPLKAF
                     EALNNLGYMESALCVLSYLQAQAFPHEKPTSFHDWIANQFGERLFSIFFKTYTEKVWG
                     MSCDEISADWAAQRIKGLDLWSAMANALRRSIAPVKSATRSNPQVKSLIESFQYPRKG
                     PGMLWEAAARHVLARGG"
BASE COUNT          476 a          825 c          912 g          396 t
ORIGIN      
        1 ccgagcgcgc gcatggttgc ctcggcttgc gcgcgcgatg tctcaagaat ggcgtgattg
       61 gccagaatgc cttggtgcaa acggtggtag ggcgcggcca cgacccggtg cggggtcagg
      121 gcgacaatgt agggcccaag atcgagatcg gcagcgacca ggccggggcg ctgggcggca
      181 agcatgcgca agctcgacgt ataaaagcag gggcggcgcg gatcggccgc cgccacggcg
      241 gcggcgccgg gccgcggggt gacgggcagg gccgcgcccg ccaggcgaat gccgccctcg
      301 agcgtggcct ggctcgcgag cacgatggcg gcgatgcgga tcaaggtgct tgaaaattgc
      361 gcgctggccg ggagtgcggc gcagaagcgg gccagcggca ggacggcaag ccagctggca
      421 taagcaacga gcttcatctg ccagcagccg agcgcgacgg cgagcagcac gatagccgtg
      481 gccagtgtgg cggcggcatc gcgccggcgt cgccacagcg cgatctgggc gccggcgcct
      541 gcgagcagaa aaacaaattg gcccagcgcg aaggccggct cccggcgcgc aaaccacagg
      601 aggctcttgg tctccagcac ctgatcaagc caaatgctat tgagggccgg atcgacctgg
      661 ccgaagggac cggcgagaca agcaggctcc agcaccgcaa aaatgcccac gccagccgcc
      721 gccgcggtgc ccaggatggc caagcgcgtg cccaacctcg cggcgaagcg ctgcgctgcc
      781 cacaatccca gtgtgccgct cgccgccagg accacgatat tgggcgacag ggcatcgcaa
      841 ttgatcttga accaggccgc cggcgccagg gttaataggc aggctgccat tagggtgagc
      901 gtggtcgccg cggccgcggg cagtatgctg agccggcgcg cgggatgaaa gagtgtcatc
      961 agcaccgcca gggcgagcgc cggcgccacc agggcgatcg cctcataacc gacggcgagg
     1021 ccgagcccga taaggccgcc ggcccaccag cctaggcggc gatcttcgat actgcgcacc
     1081 aggaatagga ggcctgcgac ggcgcagagg atctgcacgt tgtgatggtc gatgcgacca
     1141 ggccaaaatt gcgccgtggc gccgatcgag gtgacggcca ggctggcagc agccgcggcg
     1201 gcccacgctc ccccgacgcg ataggcctca cgcccgacca cggcgcatag ggtcgcgaac
     1261 aaggccgttg gccaagccag gcgcgtcagc agttcgccga gctcctgtcc gagcaggctt
     1321 cggagtgcac cgatgagcgc ggcgagcggc agatcgacca acctcgacca gtgtgaggcc
     1381 agcggttcgg gcgcgccgac gcgcgccagc gtcgggtcga accacggggc gccctcgagc
     1441 agggctcgcg cgctgacgag gcgcagcgcg tcgtcgggat cgcccagcca atcgcgcggc
     1501 gacgggccag cgccggccca agcgccgatc atgagcaagg cgacggccgc ggcggtcgtg
     1561 gcaactgcca cgccgggtcg agacggcagc gccggtgcgt gccgtgaccg ctgcgccgtc
     1621 gcaaggattg ccacgtcaac caccgtcgaa gctcgtgcag cgccattcta accaagcggt
     1681 gttagggcgg cgtaaacgca gcggcaggcc gcacggcacc cgcatccgtt cgaggtgccc
     1741 cgctcaaggc ggttcgccct acccccgcaa gggattgttc agcctttctt cctatcgtcg
     1801 ccggcgcatg gggcgcgtgc gtgcgtgggt gcgcaaggga aggcggcttg gcgctggctt
     1861 ccaaatgatg ggcggcgctc gcttcgattc aggggttgcg gcaatgtccc agtcggatgt
     1921 tttcgttatt ggggcaggac cggcaggatt aacggccgcc tatctgttga ccaagcaggg
     1981 tgtggcgacc acggtaattg agcgcgaccc gacctacgtg ggtggtatca gccgaaccgc
     2041 gagttacaag aactttctgt tcgacatcgg cgggcatcgc ttcttctcga agtcgcagga
     2101 ggtggtggcg ctgtggaagg agattttgcc tgacggtttc atcgagcggc cacgcttgtc
     2161 gcgcatctac tacaacggca catatttttc ctatccgctg aaggcgttcg aggccttgaa
     2221 caacctcggc tacatggagt cggcactgtg cgtgctctcc tatttgcagg cgcaggcctt
     2281 tccgcacgaa aagccgacga gctttcacga ttggatcgcc aaccagttcg gcgagcgatt
     2341 gttctcgatc ttctttaaga cctacaccga aaaggtgtgg ggcatgagct gcgacgagat
     2401 ctcggccgat tgggcggcgc agcgtatcaa gggtctcgac ctctggagcg ctatggccaa
     2461 cgcgcttcgc cgatcgattg cgccagtcaa gtccgccact cggtctaacc cgcaggtcaa
     2521 atccttgatc gagtcgtttc aatatccccg caaagggccg ggtatgttgt gggaagccgc
     2581 ggcccggcat gtgcttgcgc gcggcggtc
//
LOCUS       DASTMT010000317         2060 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_507602, whole genome shotgun sequence.
ACCESSION   DASTMT010000317 DASTMT010000000
VERSION     DASTMT010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2060)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2060
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_507602"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..187)
                     /locus_tag="VFR00_07420"
     CDS             complement(<1..187)
                     /locus_tag="VFR00_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012113888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU0159125.1"
                     /translation="MTSAVSGPLLAVDKLAVVYQRAITALEGISLSVAPSQMVGILGS
                     NGAGKSTLMRAISGFHGL"
     gene            complement(184..1263)
                     /locus_tag="VFR00_07425"
     CDS             complement(184..1263)
                     /locus_tag="VFR00_07425"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEU0159126.1"
                     /translation="MSRRPRASGYFRSSYGEDLTLLPTRFERWAAVGLLGALLGLPLV
                     ASPFLIDLAAQVFLAVIGALALMLLTGYAGQVSLGHAGLLAAGAFTVGILFKEFKAPF
                     FLTLPAAAAVGALLGVVFGLPSLRVRGLYLAVSTLALNFLIAYLGGEYETRRGFSTGI
                     VVDPPSLAGHSLTNPKAWYLVLLAAAVLTLLLCANLLRARSGRAWAAIRANETVAETL
                     GIGVASYKLLAFVLSSALTAVAGALFAYWRGFVSVEAFSLFLSIQYVAMIIIGGMGSL
                     AGAILGAVFVTLFPYAIEAGLSTLARGTAYAGVLFAVNAAAFGAVMIAFLLLEPQGLI
                     GLWRRLASYFLLWPFRHRPLGGARR"
     gene            complement(1256..>2060)
                     /locus_tag="VFR00_07430"
     CDS             complement(1256..>2060)
                     /locus_tag="VFR00_07430"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEU0159127.1"
                     /translation="IYRVSRVLNLAHGELMMLCAYLLLWTASLFAGHPLVAIAAALGL
                     SALVGALVYLFLLRKLTGEMALAAVLTTVALGVLLRGATVLVWSAQQQYPAQSLGASN
                     STLALPGGARISLWAALLVATTLVVYAGLFGFLRLGRWGMRMRAAGQNPLLAAQRGIN
                     LHAIYALAWSLSTLTGALAGILMALDSGLNQTMAIIGLKAFPAALVGGLDSLAGALLG
                     ALMVAATEVMLIHYVDPLLSEVVPFLLLIVVLMLRPWGLFGTREELDRV"
BASE COUNT          456 a          655 c          723 g          226 t
ORIGIN      
        1 cgaggccatg gaagcccgag atggcgcgca tcagcgtgga tttgccggcg ccgttgctgc
       61 cgagaatgcc aaccatctgg gaaggcgcca cgctgagcga aatgccctcg agcgccgtaa
      121 tggcgcgctg gtagacgacg gcgagcttat cgacggcgag gagcgggccg gaaacggcag
      181 acgtcatcgg cgcgctccgc ccaacggtcg gtgccgaaac ggccaaagca aaaagtagct
      241 ggcgaggcgt cgccacagac cgatcaagcc ctgcggctcg agcagcagga acgcaatcat
      301 gaccgcgcca aaggcggcag cgttgaccgc gaagagcacg ccggcatagg ccgtgccgcg
      361 ggcaagcgtg ctgagccccg cctcgatggc gtagggaaag agcgtgacaa agaccgcgcc
      421 gaggatggca ccggcgagcg aacccatgcc gccgatgatg atcatggcca cgtactggat
      481 ggacagaaag agcgaaaacg cttccacgga gacaaagccg cgccaatagg caaagagcgc
      541 gccggcgacc gcggtcaggg ccgagctcaa cacaaaggcc aacagcttgt agctggcaac
      601 gccgatcccc agcgtctcgg ccacggtctc attggcgcgg atggcggccc aggcgcgacc
      661 cgagcgggcc cgcaagagat tggcgcacag gaggagcgtc aggacggcgg cggcaagcaa
      721 aaccagatac catgccttgg ggttggtcag ggagtgaccg gcaaggctcg gcggatcgac
      781 gacgatcccc gtcgagaagc cgcggcgcgt ttcgtattcg ccgccgaggt aagcgatcag
      841 aaaattgagc gcgagtgtgc tgaccgccag atagagaccg cgcaccctga gcgacggcag
      901 gccgaagacg acccccaaca gcgcgcccac cgcggctgcg gccggtagcg tgaggaaaaa
      961 cggcgccttg aattccttga acaagatgcc gaccgtgaag gcgccggccg ccaacaggcc
     1021 ggcgtggccc agcgagacct ggccggcgta gcccgtgagc agcatcaggg cgagggcgcc
     1081 gatcacggcc aggaacactt gggcggcgag gtcgatcagg aacggtgagg cgacgagggg
     1141 caggccgagc aaggccccca gcaggccgac cgcggcccaa cgctcgaagc gcgtcggcaa
     1201 cagcgtcagg tcctcgccat agctggagcg gaaataaccg ctggcgcgcg gtcgcctaga
     1261 cacggtcgag ctcttcgcgg gtgccgaaca ggccccaggg ccgcagcatc agcacgacga
     1321 tcagcagcaa aaacggcacg acttccgaca gcaagggatc gacatagtgg atcagcatca
     1381 cctccgtcgc agcaaccatc agcgcgccca gcagcgcgcc ggcgagacta tcgagcccgc
     1441 cgacgagggc ggcgggaaag gccttgaggc cgatgatggc catggtctgg ttgagcccgg
     1501 aatcgagcgc catcagaatg ccggcgagcg ctcccgtcaa agtggagagc gaccaggcca
     1561 atgcatagat cgcgtgcaga ttgatgccgc gctgggccgc gagcagcggg ttctgcccgg
     1621 ccgcgcgcat gcgcatgccc cagcgtccca gtcgcaaaaa gccaaagagg cccgcgtaga
     1681 caacaagtgt ggtggcgaca agcagcgccg cccaaagcga gatgcgggcg ccccccggca
     1741 aggccaacgt gctgttgctc gccccgaggg attgggctgg atactgctgc tgggccgacc
     1801 agacgagcac ggtggccccg cgcaagagga cgccgagggc caccgtggtg agcacggcgg
     1861 ccagcgccat ctcgcctgtc aacttgcgca agagaaagag atagaccagg gcgcccacca
     1921 gcgcgctgag ccccagggcg gcggcgatgg ccacgagcgg gtgaccggcg aacagcgagg
     1981 cggtccagag caggaggtag gcgcagagca tcatcagttc gccgtgcgcc aggttgagca
     2041 cccggctcac gcggtaaatc
//
LOCUS       DASTMT010000318         3898 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_1816850, whole genome shotgun sequence.
ACCESSION   DASTMT010000318 DASTMT010000000
VERSION     DASTMT010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3898)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3898)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3898
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1816850"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..672
                     /locus_tag="VFR00_07435"
     CDS             <1..672
                     /locus_tag="VFR00_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008972861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEU0159128.1"
                     /translation="GHDVPVGAGTRIYVGDVMSLVGLSQDLERVVPKLGQALNANDRT
                     DMAFLAIGLAVGLLIGLASLSIGGIPLTLGGGGGALVAGLVCGWLRARRPTMGAYPPA
                     AQQTVIDLGLGGFVAAIGLTSGPAAVAAIQTQGLALFGAGLLVTLTPLVVGTMFAQRI
                     LGMNPVIVCGALAGAMTVDAAVTGACDVAHSQTPVLGVAVPYAVANVFLTVLGPLIVG
                     FTFAA"
     gene            complement(1321..>3898)
                     /locus_tag="VFR00_07440"
     CDS             complement(1321..>3898)
                     /locus_tag="VFR00_07440"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HEU0159129.1"
                     /translation="LTPLLVGARVHVCGDAEVQDPALLMAGIEREGITVLQVVPALLR
                     ELLRRAADEAAFQSLGQLRCLISTGEYLDLALVRAWFQRFAGVPLLNAYGLTECSDDV
                     ATHRFTTAPGAATRTSIGRPIANARLYVLDAHRQPVPAGVAGELYVGGVPVSRGYLND
                     PPQTAARFLPDPYASPGGRFLYRTGDLVRRCADDTLEFLGRVDHQIKIRGYRIEPEEI
                     EHALAAHADVQAAVVVAQHDPGTEPRLIAHIVPADGHRPAASELVDFLKMRLPAHMLP
                     AGVVFLSHLPRNAHGKVDRAALAQNGQIITTAAPAKLAPRDALEQDLTRIWQDLLGCP
                     ALGILDNFFDLGGHSLLAGRVLSRIAQAMGVQLPIRAAFEAPTIAALAGRIRQARAAA
                     GLDPREEMAFGLDDRRIPLSVAQQQMLRLERQLPDLPKFNLPFAFALRGPLNVSALAA
                     GLLEVVRRHEALRTHFPARDGEPIGLVLSPADITSALRVEEIAGLPAASRKQVSALRG
                     RRAELEIEHEAWTAFDLARAPLVRARLLRLGRDEHVLVLIFHHIVVDGWSIGVIFEEL
                     ATLYSAGATGEDAALGEPAPQFSQFVRWQRLWCATEAGTRQFDYWRAQLRGASPIFAR
                     KAQPAAALLGSAVGRETFSVPGDLLVRLGRLSRNCGATVFMALLAGFKALLLARRGCH
                     DLCVATAMANRSRPGAEHLVGPVENTTLIRTHMRPDLSFAEALGRVREAVLEAQANQD
                     LPFDVLIARLAQDGLDPTALIQAFFVLQNAFRRPFALSGVAAQSFATALPEGQPVFPL
                     DNTCLTLILQETPAGLAGTCTFKCEVLATDTLQQWLADYQMILTRAVINPASSLARLT
                     QR"
BASE COUNT          680 a         1276 c         1313 g          629 t
ORIGIN      
        1 ggccacgacg tgccggtggg cgcgggcact cgcatctacg tcggcgatgt catgagcctg
       61 gtcggcttga gccaagatct cgagcgtgtg gtgccaaagc tgggtcaggc gctcaacgcc
      121 aacgatcgaa ccgacatggc cttcctggcc attggactcg cggtgggctt gctgatcggc
      181 ctggctagtc tttccatcgg cggcattccg ctgacgctcg gtggcggtgg aggggcactg
      241 gtggccggac tggtctgcgg ctggctgcgc gcgcgccggc cgaccatggg ggcttacccg
      301 ccggcagctc agcaaaccgt catcgatctc ggcttggggg gatttgttgc ggccatcggc
      361 ttgaccagcg ggccggcggc cgtggcggcc atccaaacgc aggggctggc gctgttcggc
      421 gcgggcctcc tcgtcaccct caccccgctc gtcgtgggca cgatgttcgc acagcgcatc
      481 ctcggcatga accccgtcat tgtctgcggt gcgctcgccg gggcgatgac cgtcgacgcg
      541 gcggtgaccg gcgcctgcga cgtcgcccac agccaaactc ccgtgcttgg cgtggcggtg
      601 ccttacgcgg tcgccaacgt gtttttgacc gtcctgggtc cgctgatcgt cggctttacg
      661 ttcgccgcgt aaacctggca acttaccgac ccggccacgc gccacgggcc acgccacctc
      721 ctgcgtgcag cgcaggcgct caccgagcaa aatcagcctt ggccgcaacc gtgccgcgac
      781 gtgcgttaac gccgagaccg tgaggccttg cgacgcttta cctttttggc tttcgtcttc
      841 ctggccagtc gtttgagaga gcgatcaacg tctctgatcg ccgcctgcag gctcttctta
      901 tgatcttgca gggtctgctt cagcttttct ttttggtccg gcgtcaagtc atcgacgtcc
      961 aggtatctca gggcagggta tatcatcgcc tttcccttaa tcttcgccag tcaaaggacc
     1021 gacttcgatg gcggctgatg ggctgcgtgc gagccggact gttacgcgtg cgaggaccat
     1081 aatcaaccct aagctaacca agaacgatca acatcgttaa ggcgagttcc cccagcactc
     1141 ctggcgccgg gatcttccgc acaagtacag aggtagcggc gcgtgcccgc aaacgcgtcg
     1201 gttgctcgag cccgacccgg gcggcaactg gcccgcactg cgagcgcatg ccgtgcggcg
     1261 atggcggccg caacgccgcc gcgcgcctca cacaagagcc ctgagcaacg gcaccgcgct
     1321 tcagcgctgc gtcagccgcg ccagcgagct ggcgggattg atgacggcac gggtgaggat
     1381 catttggtag tccgcaagcc actgctgcag cgtgtcggtc gccagcacct cgcacttgaa
     1441 ggtgcatgtg ccggccaggc ctgcgggcgt ctcctgtaaa atcagcgtca ggcaggtgtt
     1501 atccaatggg aaaaccggct gaccttccgg cagcgcagtg gcgaaggatt gggcggccac
     1561 acccgacaat gcaaatggtc ggcgaaaggc gttctgcagc acgaaaaacg cctgaatgag
     1621 cgcggttgga tcgagaccgt cttgcgccag ccgcgcgatc agcacatcga aggggaggtc
     1681 ctggttggcc tgggcttcca aaacagcctc gcgcacccgg ccgagcgcct ccgcaaacga
     1741 cagatccgga cgcatgtggg tgcgaatcag cgtggtattc tccacgggcc caaccaggtg
     1801 ttccgcgccg ggccgggagc ggttggccat ggcggtcgcc acgcagaggt cgtggcagcc
     1861 gcggcgggcc agcagcaaag ccttgaaccc cgcaagcagc gccatgaaca cggtcgcccc
     1921 gcaattgcgg ctcaaccgcc cgaggcgtac gagcaaatcg ccgggaaccg aaaacgtctc
     1981 gcggccgacc gccgagccca gcagggccgc cgccggctgc gccttgcgcg caaagatcgg
     2041 cgaggccccg cgcaactggg ccctccagta atcgaactgg cgcgttccag cctctgttgc
     2101 gcaccacagc cgctgccagc gcacgaactg cgagaattgc ggggcgggtt cccccaaggc
     2161 cgcatcttcg ccggtcgcgc ctgcgctata gagcgtggcg agctcctcaa agatgacgcc
     2221 gatcgaccag ccgtcgacga cgatatggtg gaaaatcagc accagcacgt gctcgtcccg
     2281 accgagacgc aagagccggg cgcgaacgag aggggcgcgc gcgaggtcga aggctgtcca
     2341 cgcctcgtgc tcgatctcga gctcggcgcg ccggcccctg agcgcgctca cttgtttgcg
     2401 gctcgctgcg ggcaggcccg cgatctcctc gaccctgagg gcgctcgtga tatcggccgg
     2461 ggatagcacc agcccgatgg gctccccatc ccgcgccggg aagtgcgtac gtaaggcttc
     2521 gtgccggcgc acgacctcga gcaagcccgc ggcaagggcg gagacgttaa gaggtccgcg
     2581 caacgcaaag gcgaagggca ggttgaactt ggggaggtcc gggagctgcc gctcgagcct
     2641 cagcatctgc tgctgtgcga ccgacagggg aatgcggcga tcgtcaagcc cgaaggccat
     2701 ctcctcgcga ggatccagtc ctgctgccgc acgcgcctgg cggatccgcc cggccagcgc
     2761 ggcaatcgtg ggagcttcga aggccgcccg gatcggcagc tggactccca tggcctgggc
     2821 gatccgcgac aggacccggc ccgccaatag ggaatgtccg cccaagtcga aaaaattatc
     2881 gaggatgccg agcgccgggc agccaagcag gtcttgccag atgcgggtga ggtcctgttc
     2941 gagggcatcg cggggcgcca gctttgcggg tgccgccgtc gtgatgatct ggccgttctg
     3001 agccagcgcc gcgcgatcga ccttgccatg cgcattgcgc ggcaggtggg acaggaaaac
     3061 gacgcccgcg ggaagcatat gggcgggaag ccgcatcttt aagaagtcga cgagctcact
     3121 ggcagccggc cgatggccgt cagcggggac aatatgggcg atcagtcgcg gctcagtgcc
     3181 gggatcgtgt tgggccacga ccacggccgc ctgcacatcg gcatgcgcgg cgagggcatg
     3241 ctcgatttct tcgggttcga tcctatagcc tcggatcttg atctgatgat cgacgcggcc
     3301 gaggaattcg agcgtgtcat cggcgcagcg gcgcaccaga tccccggtcc gatagaggaa
     3361 acgtccgcca gggctggcat agggatcggg caggaagcgt gccgccgttt gtggcggatc
     3421 gttgagataa cctcggctca ccggcacgcc cccgacgtag agctcgcccg ccacgccggc
     3481 gggaacgggc tgcctgtgcg cgtcaaggac ataaaggcgg gcattggcaa tcggccggcc
     3541 gatgctggtg cgcgtggcgg cgcccggcgc cgtcgtaaag cggtgggttg cgacgtcgtc
     3601 cgagcactcg gtcagaccgt aggcattgag caagggcacg ccggcaaagc gctgaaacca
     3661 ggcgcgcacc agcgccagat cgaggtattc gccggtggag atcaggcaac gcagctggcc
     3721 gagcgactgg aaggccgctt cgtcggcggc ccgtcgcagg agctcgcgca gcaaggcggg
     3781 aacgacctgc aacacggtaa tgccttcccg ctcgatcccg gccatcaaca gcgcgggatc
     3841 ctgcacctcc gcgtcgccac agacatgcac ccgtgccccc accagcaggg gggtgagg
//
LOCUS       DASTMT010000319         7546 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_6325645, whole genome shotgun sequence.
ACCESSION   DASTMT010000319 DASTMT010000000
VERSION     DASTMT010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 7546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7546
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6325645"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1140)
                     /locus_tag="VFR00_07445"
     CDS             complement(<1..1140)
                     /locus_tag="VFR00_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase family protein"
                     /protein_id="HEU0159130.1"
                     /translation="MPPTETTSGPGRVLAPADSKEASIPIWLAREAGWLRHAPLSAAQ
                     KTWAEAQGTRASGGLRHVLVPGPEGRLAGAVLWLDAERSPGRARAEFSIGHFARLLPA
                     GLYHLADDAIDRELATVAWGLGAYRFRRYKTGEGEGSARLRLPTGLDAQRAQSILEGV
                     WLGRDLINTPAADLGPHELEDAARALAGRHGAHLTSIVGEDLLARNFPMIHAVGRAST
                     RPPRLIDLTWGKPGAKRVTLVGKGICFDTGGLDIKPASAMLLMKKDMAGAATALALGH
                     MIMAAGLDLRLRILIPAAENNIAGDAFRPGDVLTSRLGKTVEIGNTDAEGRLVLADAL
                     ALGDEERPDLMASFATLTGAARVALGPDLPPLYTDDDAFAVEIAEA"
     gene            1236..1901
                     /locus_tag="VFR00_07450"
     CDS             1236..1901
                     /locus_tag="VFR00_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEU0159131.1"
                     /translation="MAYALVIGSKNVSSWSLRPWLALVEAGIPFREVSIPLRRPETKA
                     EILRHSPAGHVPILIWERQGRRQPIWDSLAILEFLAESHADAGLWPSEREARAHARAI
                     CAEMHAGFRYLREHCPMDLLARTPMASLPETVAQNVRRVVELWTLSRRRFGGAGEFLF
                     GAFSAADAMYAPVASRFRTYLPDLAPYGDDGAAQAYVEAIFALPGMAKWEEGARVERA
                     GEA"
     gene            2161..2949
                     /locus_tag="VFR00_07455"
     CDS             2161..2949
                     /locus_tag="VFR00_07455"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU0159132.1"
                     /translation="MSAHDTAQTQLAGKADGEHSGKAYARDARNPEAALAFARKLRAL
                     GDKQQALLVLQQAAALHPTHRGILSEMGRLALDLEQVELAQKLLAEADDAASADWRII
                     SARGTVLAKQGKYREAIPLYERALALAPKETSLLNNLALALTMEGHAEQAESLLKQAL
                     AQGNHAARVSQNLALVLSLQGKYEEAKLVAARQLSAEAAGANVEFVRRMVQLEAKPFP
                     AMAKADPAAPSGDVSGPERAPGGEEPAASAAGWITRVAQGRGGA"
     gene            complement(2975..3325)
                     /locus_tag="VFR00_07460"
     CDS             complement(2975..3325)
                     /locus_tag="VFR00_07460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0159133.1"
                     /translation="MRARAQAAAAFRYLAFAGSVALALFNGSLYSPIYDSVDQILYLF
                     TRSSLHAGPLPLQPYRTSLFIAAMTLLIGGIPAALYERLRGLEQTTAVSAALWLLTTL
                     SLTLPALREAAGLE"
     gene            complement(3335..3610)
                     /locus_tag="VFR00_07465"
     CDS             complement(3335..3610)
                     /locus_tag="VFR00_07465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0159134.1"
                     /translation="MQHWYYHVPDLLLAAVSYLIVARLALLPFRAEGALIVRALNALT
                     APVVAGVAAITPRLLPSAAVLLAALAWLYAARIVLRTAMAATGLRLS"
     gene            complement(3782..4012)
                     /locus_tag="VFR00_07470"
     CDS             complement(3782..4012)
                     /locus_tag="VFR00_07470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0159135.1"
                     /translation="MATGKRAKLSLDRLESDREALLRRLGHLNSGAKLLPGYRSALIL
                     LNSRFRRANLATRLALLQAAQFMVEVLERIPY"
     gene            4372..5550
                     /locus_tag="VFR00_07475"
     CDS             4372..5550
                     /locus_tag="VFR00_07475"
                     /inference="COORDINATES: protein motif:HMM:NF017076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HEU0159136.1"
                     /translation="MPAEVSDRSQRGRALYPRPLLLSAVFALLSTLLLAFLLPLFAPG
                     LLRFEHAMGDLRTGLMSDRLPSQHPRIAIVAITDDTLNDLKARLPIDAGVLARTIDAI
                     DAAGAKVIGLDVLFVRTARPDNEAELIAAIRRAKAKIVLAAADERVPLSPRQRERQQA
                     FFRDSLRPAGYANLAVERDFIVRFKAQAAPGSAFAKSFAETMAETAGFAAAAQRRRIA
                     WLRPPADGSDTFLTLSADTLLRPAGDPLLGLAKAGLKGKIVIVAGMLSDIDVHRTPLQ
                     PMHGALIHAHILAGIVDGRSIGQIESGTVALPVTLAAITGLGFLIGWSLRYRRRGLIA
                     SSVATLVIIAVDTLVFWQLRIILPLVLALLAWFLGEFSGHYLGRWLGYNTDRARWFVQ
                     "
     gene            5547..6305
                     /locus_tag="VFR00_07480"
     CDS             5547..6305
                     /locus_tag="VFR00_07480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0159137.1"
                     /translation="MKLASSSLAGSALAALLMLLFAGATPARAQIYVIESSAADVKVG
                     AHLAPTDTLSLPAGAAIRAVLPSGKTQTVKGPFHGKVADLAKGQEINEGVVGWLKGIL
                     QTGGATEATPGATRSASPPARPRGQARFSWSQVPTWTDGNLCIGKDAKLQLARAPAAA
                     AQRVAVVEVESMARGEAEWAPGEALAAWPAQLAVRPGGTYWLLIEGRPRRQMKLEVFD
                     QLPGEDDVLAALQKRGCRSQFEAFVRDKIAGVTP"
     gene            6414..>7546
                     /locus_tag="VFR00_07485"
     CDS             6414..>7546
                     /locus_tag="VFR00_07485"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HEU0159138.1"
                     /translation="MAAETFDFIVTGAGSAGCAVAARLSESGRYSVLLLEAGRRDRYP
                     WIHIPLGYHKLYTHPGYNWKFESEPIAGLNNRTSYQPRGKMLGGTSSLNGLIYMRGTP
                     ADYDGWRQRGCQGWDYASVLPFFRKAENQERGEDEFHGVGGPLNVCDQPFRHELVEAI
                     IAASVEAGIPRNADFNGATQDGVGHYQATVRAGRRWSAAAAYLKPARRRKNLKVIAHA
                     HATRILIEGGRAVGLQYRGRGGVETARCRGEVIVCGGVYGSPQLLLLSGIGPAAHLKE
                     MGIPVVRDLPAVGANLHDHFNSYVALRCAKPITLNDLTRSPTAKLKAALQYAFGRTGH
                     LAGPGLYAGAFVRSDQRLDRPDLQMNFFLWSIERRDARGVYPH"
BASE COUNT         1262 a         2471 c         2567 g         1246 t
ORIGIN      
        1 ggcctcggcg atctcgacgg cgaacgcatc atcgtcggtg taaagcggcg gcagatcggg
       61 tccaagggcg acgcgcgcgg cgcccgtcag ggtggcgaag ctcgccatca gatccggccg
      121 ctcctcatcg ccgagcgcca atgcgtcggc caagaccagt cgaccctccg catccgtatt
      181 gccgatttcg acggtcttgc ccaggcggct tgtcagcaca tcgccggggc gaaaggcatc
      241 gccggcgatg ttattctccg ccgcggggat cagaatgcgc agcctgaggt cgagcccggc
      301 ggccatgatc atgtgtccaa gcgccaacgc ggtcgccgct cccgccatat ccttcttcat
      361 caaaagcatg gcgctggcgg gtttgatgtc gagaccgccg gtatcgaagc agatgccctt
      421 gcccaccagg gttacgcgct tggcgccggg cttgccccag gtcaggtcga tgaggcgggg
      481 tgggcgggtg ctggcgcgac cgaccgcatg gatcatggga aaattgcgcg ccagcaggtc
      541 ttcgccaacg atgctcgtca ggtgcgcgcc gtgccgcccg gccagcgcgc gggccgcatc
      601 ctcgagctcg tgcggtccaa ggtcggccgc cggagtgttg atgaggtcgc ggccgagcca
      661 tacgccttcg aggatcgact gggcacgctg agcgtccagc ccggtcggca accgcagtct
      721 ggcactgccc tcgccctcgc cggtcttgta gcggcgaaag cggtaggcgc cgaggcccca
      781 ggctaccgtt gccaactccc ggtcgatggc gtcgtcggcc aaatgataaa ggccggccgg
      841 cagcaggcga gcaaaatggc cgatgctgaa ctccgcccgg gcgcggccgg ggctgcgctc
      901 cgcatcgagc cagagcacag cgcctgccaa gcggccttca ggcccgggca ccaagacatg
      961 ccgcaagccg ccgctcgcgc gcgtgccctg cgcttcggcc caagtctttt gtgcggcgct
     1021 caagggcgcg tgccgcagcc agcccgcctc gcgcgccagc cagatgggga tcgatgcttc
     1081 cttgctgtcg gctggcgcca agacgcggcc aggaccagag gtcgtctcgg ttggcggcat
     1141 ggagggctgg ctcaccacaa ttgagagggg agcgctatta tcatgagcgt gagccgaaat
     1201 gacaattggc gttgagggtt gggaggtgag gagccatggc ctatgctctt gtgatcggta
     1261 gcaagaacgt gtcgagctgg tcccttagac cttggctcgc cctcgtcgaa gcgggtatcc
     1321 cgtttcgcga ggtctcaatc cccctgcggc ggcccgagac gaaggccgag atcttgcgcc
     1381 actcgccggc gggtcatgtg ccgatcctga tctgggagcg gcaggggcgg cgacagccga
     1441 tctgggatag tttggccatt ctggaatttc tggccgaatc ccatgccgac gccgggctgt
     1501 ggccgtccga gcgggaggcg cgcgcgcacg cgcgggcaat ttgcgccgaa atgcatgcgg
     1561 gcttccgcta cctgcgcgag cattgtccca tggatctctt ggcacgaacg cccatggcga
     1621 gcttgccgga gaccgtcgcg cagaacgtcc gccgtgtggt cgaattgtgg acgttgagcc
     1681 gccgccgctt cggcggcgcg ggggagtttt tgtttggtgc cttttcggcg gccgatgcca
     1741 tgtacgcccc ggttgcttcg cgctttcgca cctatctgcc tgatcttgcc ccctacggcg
     1801 atgacggggc ggcgcaagct tatgtggagg cgatattcgc cttgcccggc atggccaaat
     1861 gggaagaggg ggcgcgggtc gaacgcgcgg gggaggcttg agccgcaagg ttgcgccaag
     1921 cttaccgcct cccaatagct taagagattg ttgacggccg gccgagacac tcaagcctac
     1981 ccgcgcgaat caaggcgcgc cgcttcggaa ttctcgcaca tgacagcaaa ctggtcccgc
     2041 ccgatggccg ccgccgtaaa gcctgctagc aggggttttg ccgcgtgcat catgggcgcg
     2101 ctgctttcgg cctgcgccag cggcccaatg cccacgctcg ccaatggcga gggaggggtg
     2161 atgagtgccc acgacaccgc acagacccag ctcgccggca aagcggacgg cgagcattcg
     2221 ggcaaggcgt acgccaggga tgcgcgcaat ccggaggctg ccctcgcctt tgcccgcaaa
     2281 ctgcgggcgc tgggtgacaa gcaacaggcg ctgctggttt tacagcaggc tgcggccttg
     2341 catcccaccc atcgcggcat tctttcggaa atgggccgcc tcgccctcga tctcgagcag
     2401 gtcgagctgg cccagaagct gctggccgag gccgacgatg ccgcgagcgc ggattggcgg
     2461 atcatttcgg cgcgcggcac ggtgcttgcc aaacagggca agtaccgcga ggccatcccg
     2521 ctctatgagc gtgcgctggc gttggccccc aaggaaacct cactcctgaa taacttggcg
     2581 ctggcgctga cgatggaagg ccatgccgag caggccgaaa gcttgctcaa gcaagccctc
     2641 gcccagggca accacgcggc ccgcgtcagc cagaaccttg ccctggtgct gagcctgcaa
     2701 ggtaagtacg aggaggccaa actggtggcc gcccgccagt tgtcggcgga ggcagccggc
     2761 gcaaacgtgg agttcgtgcg gcgcatggtt caactcgagg ccaagccgtt cccggcgatg
     2821 gcgaaggccg acccagccgc gccgagcggg gacgtctcgg ggcccgagcg ggcgccgggc
     2881 ggggaggagc ccgccgcgtc ggctgccggt tggatcacgc gcgtcgccca gggcaggggc
     2941 ggagcttgag ccgaggcccc ggcgcacggg gcgttcactc gaggccggcg gcctcgcgca
     3001 gcgctggcag ggtgaggctc agcgtggtca gcaaccaaag agccgccgaa accgcggtgg
     3061 tctgctcgag ccctctgagg cgctcgtaga gcgcggccgg aatgccgccg atcagcagcg
     3121 tcatagcggc gataaagagc gaggtgcgat agggttgcaa aggtagcggg cccgcgtgta
     3181 gcgagctgcg tgtaaaaagg tagaggatct gatcaaccga atcgtagatc ggcgagtaaa
     3241 gcgagccatt gaacagggcg agcgcaacgc tgccggcgaa ggcgaggtac cgaaaggcgg
     3301 ccgccgcttg ggctctggcg cgcacggggc gagctcagct cagcctgagg ccggttgcgg
     3361 ccatggcggt gcgcagcacg atgcgcgcgg cataaagcca ggccaaggcg gccaacagca
     3421 ccgcagcgga cggcagcagc ctcggcgtta tcgccgcaac ccccgccacc acgggggcgg
     3481 tcagggcgtt cagcgcgcgc acgatcaggg cgccctcggc ccggaacggc aacagcgcca
     3541 ggcgggcgac gatcaggtag ctgaccgcgg ccaagagcag gtcggggaca tgatagtacc
     3601 agtgttgcat gaggccgagc gctcgcttgc ctgcgccttg ggggcacgtt gtccgacaaa
     3661 cggccttccc acgcaagtgc cggctctata gtcccgggcc gaggccgact tcccagccga
     3721 ccacgctcct cgcttacgac cgttgcatga acccgggccc gtgccaaccg cgaccggcaa
     3781 ctcagtaggg aattctctcc agcacctcga ccatgaattg ggcggcctgc agcagtgcca
     3841 ggcgcgtggc gaggtttgcc cgacggaacc gggagttgag caggatgagg gcgctgcggt
     3901 agccaggcag cagcttggcg ccgctgttga ggtggcccaa acggcgcaac agcgcttcgc
     3961 gatccgattc caaacgatcg agcgagagtt tggctctctt gccggtggcc aagcgagggg
     4021 cctccgcgcg cggccttggc cgtttgcggg caacggggtc ttcagttcgc tggttcaagg
     4081 gccggctccc caggatcgaa ggtggctaga gcgaaggcgc aacgcccagg ccttgcctgc
     4141 gaaggtgcca aaatatcgca taatggtttg caatatcaac atagtagccg tttggcaggg
     4201 cgcgcgcagg cgacctttcc ccctcacaac gtcgtgaacg ccgacaaccc gttgcaaccc
     4261 tccgccttag gacgattggc tcggtcgcaa ggaacaggcc accccgtcct gcagccgctt
     4321 gtggggaccc gcccgctcca ataggcttca tcggcaggga cgaggggaac catgcccgcc
     4381 gaagtcagtg atcggtctca gaggggccgc gcgctctatc cgcggccact gctgttatcg
     4441 gccgttttcg ccctgctgtc cacgctcttg ctggcgtttt tgctgccgct ttttgccccc
     4501 gggctgctgc gttttgagca cgccatgggc gacctgcgca ccggcctgat gtccgaccgc
     4561 ctgccgagcc aacatccgcg catcgccatc gttgccatca cggacgacac gctcaacgat
     4621 ttgaaggccc gtttgccgat cgatgcgggg gtgctggccc gcaccatcga tgccattgac
     4681 gcggccggcg ccaaggtgat cggcctcgac gtgctgttcg tgcgcacggc gcgacccgac
     4741 aatgaagccg agctgattgc cgcgatcagg cgcgccaagg ccaagatcgt gctggcggcc
     4801 gccgacgagc gcgtgcccct gtcgccgcgg cagcgggaac gccagcaggc gttctttcgc
     4861 gatagcctgc gcccggcggg atacgccaac ctggcggtcg aacgggactt tatcgtccgc
     4921 ttcaaggccc aggcggcccc cggctccgcc tttgccaaga gctttgccga gacgatggcc
     4981 gagactgcgg gctttgccgc tgccgctcag cgtcggcgta tcgcctggct gcgtccgccc
     5041 gccgacgggt cggatacttt cctcacgctt tcggccgaca ccctgttgcg gcctgcgggc
     5101 gatcccttgc tcggcctcgc caaagcgggc ctcaagggca agatcgtcat tgtggcgggc
     5161 atgttgtccg atatcgacgt tcaccgcacg ccgttgcagc caatgcatgg cgcgctcatt
     5221 catgcccaca ttttggccgg gatcgtcgat ggccgcagca tcggccaaat cgagagcggc
     5281 acggtggcat tgcctgtcac gctggccgcc atcaccgggc tcggctttct gatcggctgg
     5341 tccttgcgtt accgccgccg cggcttgatc gccagcagcg tggcgaccct cgtcatcatt
     5401 gctgtcgaca cgctggtgtt ctggcaactg cgcatcatct tgccgctcgt gctcgccctg
     5461 ctggcctggt tcctgggcga attctccggc cattatctcg gccgatggct tggctacaac
     5521 accgatcgag cgaggtggtt tgtgcaatga aactggcttc ttctagcctt gccggcagtg
     5581 ctctcgcggc gctcctcatg ctgctgtttg ccggcgcaac gccggcgcgg gcgcagattt
     5641 acgtaatcga atcgagcgca gccgacgtca aggtcggcgc gcacctcgcc ccgaccgata
     5701 ccctctcgct gcccgcaggc gccgccattc gcgccgtcct gccgtccggc aagacgcaga
     5761 cggtcaaagg tccctttcat ggcaaggttg ccgaccttgc caagggacag gagatcaacg
     5821 agggcgtggt gggctggctc aagggcattt tgcagaccgg cggagcgacg gaagccaccc
     5881 ctggagccac gcgcagcgcc agccccccgg cccggccgcg cgggcaagcg cgcttctcat
     5941 ggagccaagt gccgacctgg accgacggca atctttgcat cggcaaggac gccaagttgc
     6001 agttggcgcg cgcgccggca gcggcggcgc agcgcgtggc cgtggtcgag gtggaaagca
     6061 tggcgcgcgg cgaggcggaa tgggcgccgg gcgaagcgct cgcggcctgg ccggcacagc
     6121 tggccgtgcg gcccggcggc acctattggc tgctgatcga gggccgaccg cgccggcaga
     6181 tgaagctcga ggtcttcgac cagctgcccg gcgaggacga cgtgctggcg gccctacaaa
     6241 agcgcggctg ccgctcccag ttcgaagcct tcgtgcgcga caaaattgcc ggggtgacgc
     6301 cgtagcctgg cgttgcctgc cgcccggccg ttgcggtgca ccacggtgac gtgcggcaac
     6361 cctgacgtcg gcccagggca aagaaaggat cggcggctca gccaggagac agcatggcgg
     6421 cggagacctt cgatttcatt gtcacgggcg cgggttcggc aggctgtgcg gtcgcagcaa
     6481 ggttgtcgga gtcgggccgc tacagcgtgc tcctgctcga ggccgggcgg cgcgaccgtt
     6541 atccctggat ccatatcccg ctcggctatc acaagctcta tacccatccc ggctacaatt
     6601 ggaagttcga gagcgagccg atcgccggcc tcaacaatcg cacctcctac caaccccgcg
     6661 gcaagatgct gggcggcacg agctccctca acggcttgat ctacatgcgc ggcacgccgg
     6721 ccgactacga cggttggcgc cagcgcggtt gtcagggctg ggactatgcc agcgtgctgc
     6781 cgtttttcag gaaggccgaa aaccaggagc gtggagaaga cgagtttcat ggggtcgggg
     6841 gaccgctcaa cgtttgcgac caaccgtttc gccacgaact cgtcgaggcc attatcgcag
     6901 cgagcgtcga agcgggcatc ccgcgcaacg cggacttcaa cggcgcgacc caggacgggg
     6961 tcgggcatta tcaggcaacg gtgcgggcgg gacgccgatg gagcgcagcc gccgcctatc
     7021 tgaagccggc gcgcaggcgc aagaacctca aggtgatcgc gcacgcccat gccacgcgca
     7081 tcctgatcga gggcggacgg gccgtcggcc tgcaataccg cgggcggggt ggcgttgaga
     7141 ccgcgcgttg ccgcggcgag gtgatcgtgt gcggcggcgt ctatggttca ccgcagctgt
     7201 tgctgctgtc ggggatcggg ccggcggccc acctcaaaga gatgggcatt cccgtcgtgc
     7261 gagacttgcc ggccgtgggc gccaacctgc acgatcattt caattcctac gtggccttgc
     7321 gctgcgccaa gcccattacg ctcaacgacc tgacgcggag cccgaccgcc aagctgaaag
     7381 ccgcgctgca atatgccttc ggccgcacgg gccatcttgc cggccccggt ctttacgcgg
     7441 gtgccttcgt gcgcagtgat cagcgcttgg atcggcccga tctgcaaatg aactttttcc
     7501 tatggagcat cgaaaggcgc gatgcgcgcg gcgtctaccc gcacaa
//
LOCUS       DASTMT010000320         1673 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_508359, whole genome shotgun sequence.
ACCESSION   DASTMT010000320 DASTMT010000000
VERSION     DASTMT010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 1673)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1673)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1673
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_508359"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1185
                     /locus_tag="VFR00_07490"
     CDS             <1..1185
                     /locus_tag="VFR00_07490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0159139.1"
                     /translation="PSPQAVAGRYWRRLGPCLLAVALIAHAAYAHDPSSYGGVFRSRN
                     LGANWLSADVGQFLNAALIVAVNPNDQTHLLAGTDLGLIGSRNAGLSWKPEARDLIYG
                     AVFAVTFLDDGRGAICAAQNGVFRFAAGHWQRSLAPDAALPAKTLLTSRSGHRVYLLG
                     RNRLFISTDAGESFAPQPTPSPASEITALTVVGSEPELILAVIDGQVMANFEGSQQWR
                     ARGLGQESEPVDIVAADPHVPRRIWAAQTGHIHVSDDLGEAWRRVGHPLPEPATLVRG
                     IAANGEATTLLVSTNRGLYRSENGGETWALKEDNLPIHLEAGPLARDPREAGLIYAVY
                     SLMPYAEVWRLANEGGSLIERLDPISLAGGVALWLLILLAGAWLAQRLAHGRAATRGD
                     LK"
     gene            1248..>1673
                     /locus_tag="VFR00_07495"
     CDS             1248..>1673
                     /locus_tag="VFR00_07495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0159140.1"
                     /translation="MFLGALVKLLQGRAPAGKFVEYAMPHADEAPTAIAAAVDGTVWF
                     TIDRADAIGRWRDGHIDRLATPRRNFEPLGLAIAADGSAWYTDIAAGAVVRMAPSGEA
                     APFALNSPITRLGRLAVAPDGSVWFADASGWGITQLANGT"
BASE COUNT          255 a          590 c          538 g          290 t
ORIGIN      
        1 ccctcgcctc aggccgttgc gggccgctat tggcgccgtc tcggtccttg cttgctggca
       61 gtggcgttga tcgcccatgc cgcgtacgcg cacgatccaa gctcctatgg cggggtgttt
      121 cgctcgcgca atctgggcgc aaattggctc agcgccgacg tgggccagtt tctcaatgcc
      181 gccctcatcg tcgccgtcaa cccgaacgac cagacccacc tcttggccgg gaccgatctc
      241 ggtctcatcg gctcgcgcaa cgccgggctg agctggaagc cggaagcccg cgatctcatt
      301 tacggcgccg tgttcgccgt gaccttcctg gacgacggcc gaggtgcaat ttgcgccgcc
      361 cagaacggcg tttttcggtt tgctgccggc cactggcaaa ggtcgctcgc gccggacgcc
      421 gcgctacccg ccaagacctt gctgaccagc cgctcgggtc atcgtgttta tttgctcggc
      481 cgcaatcgtc tcttcatcag cactgacgcg ggcgagagct ttgcacctca accgacgccg
      541 tcgccggcaa gcgaaatcac cgcgctcacc gtcgttggct ccgagcccga gctcatcctt
      601 gccgtgatcg acggccaggt catggccaat ttcgagggga gccagcaatg gcgcgcgcgt
      661 ggcctcggac aagaaagcga acccgtggac atcgtcgcag cggacccgca cgtgccccgg
      721 cgcatctggg ccgcccagac gggacacatc cacgtcagcg acgatcttgg cgaagcctgg
      781 cgaagggtcg gtcacccctt gcccgagcct gcaaccctcg tgcgtggcat tgccgcgaac
      841 ggggaagcaa cgaccctgct cgtcagcacc aatcgcgggc tctatcgcag cgagaatggt
      901 ggcgagacat gggcgctcaa agaggacaat ctgccgatcc atctcgaagc cgggccgctg
      961 gcgcgcgatc cgcgcgaggc gggcctcatc tacgccgtct attccctcat gccctacgcg
     1021 gaggtgtggc gtctcgccaa cgagggcggc agcctgatcg agcgccttga cccaatcagt
     1081 ctggctggag gcgtcgcgct ctggctcctc atcttgctgg cgggcgcctg gctggcccag
     1141 cgcttggcgc acgggcgtgc ggccacgcgc ggcgatctca agtgatgctc gtcgcgaccc
     1201 caacaacccg gcgcgggttg gcggtgcttg ccgcccttgg acttgccgtg ttcctcggcg
     1261 ccctcgtcaa gctgctgcaa ggccgggcgc cggcaggtaa gtttgtcgaa tacgcaatgc
     1321 cccatgccga cgaggcgccc acggccattg ccgccgccgt cgacggcacc gtctggttca
     1381 ccattgatcg cgccgacgcg attgggcggt ggcgcgatgg ccatatcgac cggctggcga
     1441 cgccgcgccg caacttcgaa cccctgggcc tcgcgatcgc agccgacggc agcgcctggt
     1501 acacggatat cgcagccggt gcggtcgtgc gcatggcccc ctcgggcgaa gcagccccct
     1561 ttgcccttaa cagcccgatc accaggttgg gtcggctggc cgttgcgccg gacggctcgg
     1621 tgtggttcgc cgatgccagc ggctggggca tcacgcagct cgcaaacggc acg
//
LOCUS       DASTMT010000321         1686 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_5997844, whole genome shotgun sequence.
ACCESSION   DASTMT010000321 DASTMT010000000
VERSION     DASTMT010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 1686)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1686)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1686
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5997844"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..431)
                     /locus_tag="VFR00_07500"
     CDS             complement(<1..431)
                     /locus_tag="VFR00_07500"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEU0159141.1"
                     /translation="MTQLLRGLVSGTRSLAPAALDQRVRRVASGFSALGVKAGDCVAL
                     LMRNDIAFIEASFAAQTVGAYAVPINWHFKAEEIAYILADSAAKVLVGHADLLAPIGR
                     ELPAGVSAIAVATPPEIAAAYGLAPLAPGAVAGALDYQAWLA"
     gene            660..1682
                     /locus_tag="VFR00_07505"
     CDS             660..1682
                     /locus_tag="VFR00_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011089417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEU0159142.1"
                     /translation="MLLGSAVLAQGPAPKEALWTLRDFKFHTGEVLAELNLAYTTLGD
                     PAGEPVLILHGTTGSGGGLLAPAFGGELFGPGQPLDASKYFIMLPDAIGHGKSSKPSD
                     GLRAAFPRYTYDDMVSAQYRLVTEGLGVKHLRLVLGNSMGGMHAWLWGTHYPEFMDAL
                     VPMASQPTELSSRNWMLRRMIIDAIRNDPDWKGGQYTSQPRGLQVAAVFYGIATSGGT
                     LAWQKQAPTRALADKLLDARLATPFKADANDYLYQWESSRDYDASPGLERIKAALLAI
                     NSADDERNPPETGLMERELKRVPGAQLYLIPASEETRGHGTTGLAKFWKLKLQEFLAS
                     APRRTM"
BASE COUNT          284 a          561 c          569 g          272 t
ORIGIN      
        1 gcgagccacg cctgatagtc gagggcgccc gccaccgcgc cgggcgcaag cggcgccagg
       61 ccataggcgg cggcaatttc cggcggtgtg gcgaccgcga ttgcggacac gccggcgggc
      121 agctcgcggc cgatcggggc cagcaaatcc gcgtggccga ccaaaacttt ggcggcgctg
      181 tcggccagga tgtaggcaat ctcctcggct ttgaagtgcc agttgatggg aacggcatag
      241 gctcccacgg tctgggcggc aaacgaggcc tcgatgaagg cgatgtcgtt gcgcatgagg
      301 agggcgacgc agtcgcccgc cttgacgcca agcgcggaga aaccggaggc aacgcgccgc
      361 acccgctgat ccaacgccgc cggtgccagt gaacgtgtgc cgctgacgag cccacgcaag
      421 agctgggtca tgattgcccc gtcgccgtcc gattgccccg tcgccgtcta aatgaccgcg
      481 gcgagcttag cattgctcgg cgcgcctgcc agccgtcacg cgcgccccat ggcctcgggc
      541 cgtcgcctcg tttaggctgc gggcgacggt gcggctcgcg gagacgcgaa gctttgaaag
      601 ggtgttccat gcgcaatcga tgtgccctgc cgggagccgg cttggtcctg ggtttggtca
      661 tgctgctggg gtcggcggtg ctggcccagg gcccggcccc gaaggaagcg ttgtggacgc
      721 tgcgcgactt caagttccat accggggagg tgttggccga gctcaacctt gcctacacca
      781 ccttgggcga tccggccggc gagcccgtcc tcattctgca cggcaccacg ggctcgggcg
      841 gcggacttct cgcgcctgcc ttcggcggcg agctattcgg ccccggccag ccgctcgatg
      901 ccagcaaata ctttatcatg ctgcccgacg cgattggaca cggcaaatcc tccaagccgt
      961 cggatgggct gcgcgcggcg ttcccgcgct acacctatga cgatatggtg agcgcgcaat
     1021 accgcctggt gaccgaaggt ctcggggtca agcacctgcg ccttgtcctg ggcaactcga
     1081 tgggcggcat gcacgcttgg ctgtggggca cgcactatcc cgagttcatg gatgcgctgg
     1141 tgccgatggc ctcgcaaccg accgagttgt cgagccgcaa ttggatgctg cggcgcatga
     1201 tcatcgatgc cattcgcaac gatcccgact ggaaaggggg ccagtacacg agccagccgc
     1261 gcggcctgca ggtggccgcg gttttctatg gcatcgccac cagcggcggc accttggcct
     1321 ggcaaaagca ggcgcccaca cgcgcgcttg ccgacaagct cctcgatgcg cgactggcga
     1381 cccccttcaa ggccgacgcc aacgactact tgtaccaatg ggagtcctcg cgcgactacg
     1441 atgcctcacc ggggctggag cgcatcaagg cggcgctgct cgcgatcaac tcggccgatg
     1501 atgagcgcaa tccccccgag accggcctca tggaacgcga gctgaagcgc gtgccggggg
     1561 cccagcttta tctgatcccg gcgagcgagg agacacgcgg acacggcacc accgggctcg
     1621 cgaagttctg gaagctgaaa ctccaggagt tcctggcctc cgcgccgcgg cggacaatgt
     1681 gacgct
//
LOCUS       DASTMT010000322         5671 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_5669148, whole genome shotgun sequence.
ACCESSION   DASTMT010000322 DASTMT010000000
VERSION     DASTMT010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 5671)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5671)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5671
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5669148"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            338..1438
                     /gene="mtnA"
                     /locus_tag="VFR00_07510"
     CDS             338..1438
                     /gene="mtnA"
                     /locus_tag="VFR00_07510"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012111166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HEU0159143.1"
                     /translation="MNIAGKAYRTIWLAADGWSVDIIDQTQLPHALRTVTLRTLEDAA
                     RAIKTMQVRGAPLIGATAAYGVCLALRQDSSEAALLRAVALLAEQRPTAVNLRWALAE
                     MRAAVRNLPPDRRVAAAYRRAAEICEEDVKTNRQIGRNGAELIQEIAARKPPGERVNI
                     LTHCNAGWLACVDRGTALAPIYESWDAGVPLHVWVDETRPRNQGASLSAYELGAHGVP
                     HTIIVDNAGGHLMQQGLVDLCLVGADRVTRNGDAANKIGTYLKALAAQDNAVPFYVAV
                     PGSTIDWTLSAGTEIPIEQRSAEEVLVMTGRTADGQVVPVEIAAAGSPARNDAFDVTP
                     ARLITGLITERGVGRATPEGLERLFPEARQGE"
     gene            complement(1523..2395)
                     /locus_tag="VFR00_07515"
     CDS             complement(1523..2395)
                     /locus_tag="VFR00_07515"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012111424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HEU0159144.1"
                     /translation="MAIAVTFLTPAVGAAISGVDLARLTDGEFAQIAEIWHRRSALLF
                     RGQRIGDADLIDFSRRFGELDPPPNQERGRLSPPGYPEIYVVSNVRDASGQPIGALGA
                     GEAVWHTDMSYLELPPDASMLYALEIPPHGGDTWICGMEAAWAALPAPVQERVRGRRI
                     KHDGTYNSGGFLRQGVTASDDPHAAPGAWHPAVCRHPVTGMPSLYLGRRRNSYIEGLS
                     LAESESLLDVLWAHIDRPELRYEHKWRVGDLLLWDNRSTMHRRDPFDQTARRVMHRTQ
                     IKGAHRPQPFSAAA"
     gene            complement(2495..4057)
                     /locus_tag="VFR00_07520"
     CDS             complement(2495..4057)
                     /locus_tag="VFR00_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014495966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HEU0159145.1"
                     /translation="MSWQEELDELRARELMAREMGGADKVRRQHEGGRLTVRERIEAL
                     IDAGSFHEIGAIAGKAEYDADGQLTRLTPSNCVMGRARIEGRPIVVVGDDFTVRGGSA
                     DATIPTKPIMAERMAGELRLPIVRIIEGSGGGGSVKTIETSGRANLPGGLGQSSIGFQ
                     LLAETLATVPVVALGLGSVAGLGAARLAASHYSVMTKTTSAMFVAGPPVVARAGQQLD
                     KQTLGGWEVQCRAGAVDHAVETEAEAFQCARRFLSYLPSSVYDVAERGERRDPPERRE
                     DMLFSVVPRDRRKVYRMRPILEAVVDRGSLFEVGGMFGRSIITALARLDGLPVAVLAS
                     DPYHYGGAWTAEACQKIVRFVDMAETFHLPVVYLADCPGFLIGLDAERSATIRHGVRA
                     MAAINQSSVPWCTIIVRNAFGVAGAAHVPAGRLALRYAWLSGWWGSLPLEGGIEAAYR
                     AEIDAAPDAKAKLAEIETRLNQLRSPFRTAEAFWVEQIIDPRDTRPLLCEFANLAAPL
                     RTPGPRTFPMRP"
     gene            complement(4085..5320)
                     /locus_tag="VFR00_07525"
     CDS             complement(4085..5320)
                     /locus_tag="VFR00_07525"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEU0159146.1"
                     /translation="MREGQQAAADTDLPLRGIRILDVASFIAAPVAATVLGDYGADVI
                     KVEPVLDGDPNRLISVLSPGYPKCSVNYPWHLDSRGKRSLAIDLKNVSARALFDRVIA
                     SADVLITNYPASSRQRLRLTYEDVAKVNARLIYASLTGYGESGPEADQMGFDINAYFA
                     RSGIYDGSRYDDAAPGVSMPAQGDRPAGIALAAGIMIALWQRERTGRGSKVASSLLAN
                     GMWANANAIQAALLGADLPRRPPPERPRSALANVYRTQDERWFQLTLVREDRDWPALC
                     RAVGRADLLQDARFADTPSRRSHAPALAAALRALFATRPFAHWRARLKENGIAFACIA
                     RAQDFAADEQAVAAGILVETANPEMPRSIAAPFSIADVKLPPARPGPAHGAHSAEVLR
                     EVGIGAAEIAALTAAGALR"
     gene            complement(5389..5667)
                     /locus_tag="VFR00_07530"
     CDS             complement(5389..5667)
                     /locus_tag="VFR00_07530"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU0159147.1"
                     /translation="MINTARGPVIDEAALLQTLSAGRLFGAGLDVFAQEPPPSQHPLL
                     KLDNVVLTAHFAGPTWDNHVARFRNAFDNIERVARGEAPLWLVPELAG"
BASE COUNT          978 a         1904 c         1930 g          859 t
ORIGIN      
        1 ctccccgggg cgagaaggtc ccggggcgag aagctccccg gggcgagaat acccggccgc
       61 ggcgcccgcg gcgctgagcc gccgcgtgct gccggcaagg cgcgccggca aaagcgcgtg
      121 gggatgttgc gaattgcgcg aaaggcgcca tatagcgctc gcgcgcagct tgcaatgcgt
      181 gctagcgcgc ggcttgcaat ggaaggcttg ccatgattga gcgcctggca tcccgtttgc
      241 ccgtgactcc ggccgctggc cgccggcaaa gggctgatgg tcgccgcagg gcggcagcag
      301 ggatcatgcc cagcagggct tgtgggggca cccgtccatg aatattgccg gcaaggccta
      361 tcgcacaatc tggctcgcgg ccgatggctg gtcggtggac atcatcgatc agacgcagct
      421 accgcacgcg ttgcgcaccg tgacactgcg cacgcttgag gatgccgcac gcgccatcaa
      481 gaccatgcaa gtgcgcggcg cgccgctgat cggggccacg gccgcctacg gcgtgtgcct
      541 ggccttgcgc caggacagct ccgaggcggc gctcctgcgc gccgtggcgc tcctcgccga
      601 acagcgccca accgcggtca atctgcgctg ggcgctggca gagatgcgcg cggccgtgcg
      661 caacctgccg cccgatcgcc gggtcgcggc agcctaccgc cgcgccgcgg agatctgcga
      721 ggaagatgtc aagaccaacc ggcaaatcgg gcgcaacggg gctgagctca tccaggagat
      781 cgccgcgcgc aagccgccgg gcgagcgggt gaacattctc acccattgca atgccggctg
      841 gctcgcctgc gtcgaccggg gtacggcgct ggcgccgatt tacgagagct gggacgccgg
      901 cgtacccctg cacgtttggg tggacgagac gcgcccccgc aatcagggcg cctcgctgtc
      961 ggcctacgag ctcggggccc acggcgtgcc gcacacgatc atcgttgata atgcgggcgg
     1021 acacctcatg cagcagggtc tcgtcgacct ttgcctggtc ggcgccgatc gcgtcacgcg
     1081 caacggcgat gctgccaaca agatcggcac ctatctcaag gccttggccg ctcaggacaa
     1141 cgccgtgccg ttctacgtgg ccgtgcccgg ctcgaccatc gattggacac tgagcgccgg
     1201 caccgagatt ccgatcgagc aacgcagcgc cgaggaagtg ctcgtcatga ccggtcgcac
     1261 cgccgacgga caggtcgtac cggtcgaaat tgccgccgcc ggtagccccg cccgcaacga
     1321 tgccttcgac gtgacaccgg cgcggctgat cacgggtctg atcacggaac gcggcgttgg
     1381 ccgtgccacg ccggagggtc tcgagcgcct atttccggag gcgcggcagg gcgaatagga
     1441 tcggccgccc ttcaggcttt gcgcgcgccg gctcggcggg gcgccgaacg cttcgggcag
     1501 cgcagcaggg tgggccgacc cgtcaggcgg cggccgaaaa gggctgcggc cgatgcgcgc
     1561 ctttgatttg cgtgcggtgc atgacacggc gcgctgtctg atcgaaggga tcgcgacggt
     1621 gcatggtcga ccgattgtcc cacaacagga gatcgccgac ccgccatttg tgctcgtagc
     1681 gcagctcggg ccggtcgatg tgcgcccaca gcacatcgag cagcgactcc gattcggcca
     1741 ggcttaaccc ctcgatgtag gaattgcggc gccggccgag gtagaggctc ggcattcccg
     1801 tgacgggatg ccggcacacc gccgggtgcc aggcgcccgg cgcggcgtgc ggatcgtcgc
     1861 tggctgtcac gccttgacgc agaaatccgc cgctattgta ggtgccatcg tgcttgatgc
     1921 gccgaccccg cacccgctcc tgcacggggg cgggcaaggc agcccaagcg gcctccatgc
     1981 cgcagatcca ggtatcgccg ccgtgggggg gaatttccag ggcgtagagc atcgaggcgt
     2041 cagggggcag ctccaggtag ctcatgtccg tatgccacac cgcctcgccg gctcccagcg
     2101 ctccgatcgg ctgaccgctg gcgtcgcgaa cattcgagac gacgtagatc tcgggataac
     2161 ccggcgggct gagccggccg cgttcctggt ttggcggcgg gtcgagctcg ccgaagcggc
     2221 ggctgaaatc gataagatca gcatcgccga tgcgctggcc gcgaaacagc aacgccgagc
     2281 gccggtgcca gatctcggca atttgcgcaa actcgccgtc cgtcaagcgt gcaagatcca
     2341 cgcccgagat cgccgcaccg acggcggggg tcaagaacgt caccgcaatg gccatgggtc
     2401 gcctccggat cggcgctttc gcgctttgcg cgcacgaggg gctggagctg caggcaacgg
     2461 tacccgggcg cgcggtgaac gcaaccctat gctgctaggg tcgcattgga aatgtccggg
     2521 gccccggcgt gcgcaatggc gcggcaaggt tggcgaactc gcacaatagc gggcgggtat
     2581 cgcgcggatc gatgatttgc tcgacccaga aggcctcggc ggtgcggaac ggcgagcgca
     2641 attggttgag ccgcgtctcg atttcggcga gctttgcctt tgcatcgggc gccgcgtcga
     2701 tctcggcgcg gtaggcggct tcgatgccgc cctcgagcgg aagcgaaccc caccaacccg
     2761 acagccaggc atagcgcagg gccaaccggc cggccggcac gtgcgccgcc cccgccacgc
     2821 cgaaggcatt gcgcacgatg atcgtgcacc agggcacgct tgactggttg atggcagcca
     2881 tggcgcgtac gccatggcgt atggtggcgc tgcgctccgc atcgagaccg atcaggaagc
     2941 cgggacagtc ggccaaatac acgacaggca agtggaaggt ctcggccatg tccacaaagc
     3001 ggacaatctt ctggcaggcc tccgccgtcc aggccccgcc gtagtgatag ggatcgctcg
     3061 ccagcaccgc caccggcagc ccgtccagcc gcgccaacgc cgtgatgatc gagcgcccga
     3121 acatgccgcc gacctcgaac aaggagcccc gatcgacgac ggcctcgagg atcggacgca
     3181 tgcggtaaac cttgcgccgg tcgcgcggga ccacgctgaa gagcatgtct tcgcggcgct
     3241 cgggcggatc gcgacgctcg ccgcgttcgg caacgtcata gactgacgag ggtaggtagg
     3301 acagaaagcg acgggcgcac tgaaatgcct ccgcttcggt ctccacggcg tgatccacgg
     3361 caccggcgcg acactgcacc tcccagccac caagcgtctg cttgtcgagc tgctggcccg
     3421 cgcgtgcgac cactggcggt ccggccacga acatcgccga tgtcgtcttc gtcatcaccg
     3481 aatagtggct ggcggcgagc cgcgcggcgc cgaggcccgc caccgagccc aagcccaatg
     3541 ccaccacggg aacggtggcc aaagtctcgg ccagcagctg gaagccgatg ctcgactgcc
     3601 cgagtccacc gggcaggttg gcgcggcccg aggtctcgat ggtcttgacc gagcccccgc
     3661 cccccgatcc ctcgatgatg cgcacgatcg gcaggcgcag ctcgccggcc atgcgctcgg
     3721 ccataatcgg cttggttgga atggtcgcat cggccgagcc gccccgcacc gtgaaatcat
     3781 cgccgacgac gacgatcgga cgaccctcga tccgggcgcg acccatcacg cagttggagg
     3841 gggtcagtcg cgtcaactgc ccgtccgcat cgtattcggc cttgcccgca atggcgccga
     3901 tttcatggaa gctgccggcg tcgatcaacg cctcgatgcg ctcgcgcacg gtgaggcgcc
     3961 cgccctcgtg ctgtcgccgc accttgtcgg cgccacccat ttcgcgcgcc atcagctcgc
     4021 gcgccctgag ctcgtcgagc tcttcttgcc agctcatgcg tattaccctc gggatacttc
     4081 ctattcagcg caaggcaccg gcggccgtga gcgcggcgat ttccgccgcg ccgataccaa
     4141 cctcgcgcag cacctcggcg gaatgggcgc cgtgcgcggg gcccggacgg gccggcggca
     4201 gcttgacatc ggcgatggaa aaaggtgcgg caatgctgcg cggcatttcg ggatttgcgg
     4261 tttcgaccaa aatgccggcg gccacggcct gctcgtccgc ggcaaagtcc tgcgcgcggg
     4321 cgatgcacgc aaaggcgatg ccgttctcct tcagccgcgc ccgccaatgc gcgaagggcc
     4381 tagtcgcaaa aagcgcgcgc agggcagctg ccagcgcggg ggcatggctg cgccggctgg
     4441 gcgtgtcggc aaagcgtgca tcctgcaaga gatcggcgcg cccgacggca cggcaaagtg
     4501 cgggccagtc gcgatcttcc cgcaccagcg tcaattgaaa ccagcgctcg tcctgggtcc
     4561 gataaacgtt ggcaagggca ctgcgcggcc gctcgggcgg gggccgtcgc ggtaaatcgg
     4621 cacccagcaa ggcggcctgg attgcattgg cgttggccca catgccattg gccaatagcg
     4681 agcttgcgac tttgctgccg cgcccggtgc gttcgcgctg ccaaagcgca atcatgatgc
     4741 cggcggcgag cgcgatgccg gcgggacgat caccctgcgc cggcatactg acgccgggag
     4801 cggcatcatc gtagcgcgag ccatcgtaaa tacccgagcg cgcaaaatag gcattgatgt
     4861 cgaatcccat ctgatcggcc tcgggcccac tttcgccata accggtgagc gaagcataga
     4921 tcaagcgcgc gttgaccttc gccacgtcct cgtaggtaag gcgcaggcgt tggcgggagc
     4981 tggcgggata gtttgtgatc agaacatcgg ccgaggcgat cacgcgatcg aagagagcgc
     5041 gcgcagagac gtttttcaga tcgatcgcca gcgagcgctt gccgcgggaa tcaaggtgcc
     5101 aggggtagtt gacggaacat ttgggatagc cgggcgaaag caccgagatc agccgattgg
     5161 gatcgccgtc gagcacgggt tccaccttga tgacgtcggc accatagtcg ccgagcaccg
     5221 tcgccgccac cggggcagcg atgaaggagg cgacatcgag aatgcggatg ccgcgcagcg
     5281 gcaggtcggt atcggctgcc gcctgctgcc cttcgcgcat gtgcctgcct ccggcgctcc
     5341 cctggctctt cggcaagcgt gaaggccctc gccgcccggg tcaagggctc agccggcaag
     5401 ctcgggcacg agccagagcg gcgcctcgcc acgcgcgaca cgctcaatgt tgtcaaacgc
     5461 gttgcgaaaa cgcgccacgt gattgtccca ggtcggcccg gcaaagtgtg cggtgagaac
     5521 gacgttatcg agcttgagca gcggatgctg cgacggcggg ggttcttggg caaaaacatc
     5581 caggcccgcg ccgaacagcc ggcccgcgct cagggtttgc aacagcgccg cttcgtcgat
     5641 cacggggccg cgcgccgtat tgatcaaaat c
//
LOCUS       DASTMT010000323         1711 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_5669614, whole genome shotgun sequence.
ACCESSION   DASTMT010000323 DASTMT010000000
VERSION     DASTMT010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 1711)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1711)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1711
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5669614"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            191..1384
                     /locus_tag="VFR00_07535"
     CDS             191..1384
                     /locus_tag="VFR00_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018457338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase/NADH oxidase"
                     /protein_id="HEU0159148.1"
                     /translation="MAQPLLFTPLSLKSVSLKNRVVVAPMHQYAAVNGHATDWHLVNA
                     GRYAQGGAGLVIMESTKVARNGCGTVGDVGLWHDAFIPALARCAQFIKAHGAVAGIQL
                     GHSGRKARLQRPWEGGKPLTGGEPEIFDWDGWELVAPSAVPFSAQSPTPRALSTSEVR
                     ELVARWGEAAVRAAKAGFDVIEIHGAHGYLVHQFLSPVANQRGDAYGGTEANRMRFAL
                     EVAECVRAAWPGDKPLFMRLSCEDDAGWGPNESVRLARLLKGAGVDVIDCSSGGTLGQ
                     SPMDGARAKRYGYQVPYAEKIRREADIMTMAVGHIVHADQAETILREGRADLIALARE
                     IMHNPSWPMDAAQKLGVDPQFRLVPPAYAYWLAKRANSGFEGTPSTWSKGLAAGAAAK
                     AADKA"
BASE COUNT          288 a          552 c          603 g          268 t
ORIGIN      
        1 ccgggagaga cgcgacgcgt cgtggccttg gcgctatcgg ccgccctcaa cgcgccgatt
       61 ccggcacccc gcttcggcgt ctttcgcagg tagagggccg cggcggaccg gctgccttcg
      121 gcaggccggg cgcgcgcggc gcaagggcaa ctcggcccga tcgggccacg cgtcaatggg
      181 accgctacga atggcacaac ccctgctctt cacgccgctc tcgctcaaga gcgtaagcct
      241 caagaaccgg gtggtggtcg caccgatgca tcaatacgcg gcggtcaacg gccacgccac
      301 cgattggcat ctcgtcaacg ccggacgcta tgcccaaggc ggcgcgggac tggtcatcat
      361 ggaatcgacc aaggttgcgc gcaacggttg cggcacggtc ggcgatgtcg gtctgtggca
      421 tgacgccttc atcccggccc tggcgcggtg cgcgcagttc atcaaggcgc acggtgccgt
      481 tgctggcatt cagctcggcc actcgggccg caaagcgcgc ctgcagcggc cgtgggaagg
      541 cggcaagccg ctgacgggcg gcgagcccga aattttcgat tgggacggct gggagctggt
      601 ggccccgagt gcggtgcctt tcagcgcgca gtcgccgacg ccgcgagctt tgtcgacgag
      661 cgaggtgcgc gagttggtgg cccggtgggg cgaggccgcc gtgcgggcgg ccaaggctgg
      721 cttcgatgtc atcgaaatcc acggtgcgca tggctatctg gtgcaccagt tcctgtcacc
      781 ggttgccaac cagcgcggcg atgcctacgg cggcacggag gccaatcgca tgcgctttgc
      841 cctcgaggtt gccgagtgcg tgcgcgccgc ctggcccggc gacaagccgc tgttcatgcg
      901 cctgtcgtgc gaggacgacg ccggctgggg tccaaacgag agcgtcaggc tggcgcggct
      961 gttaaagggc gcgggcgtcg acgtcatcga ttgttcgtcg ggcggcacct tggggcagtc
     1021 gccgatggat ggcgcacgcg ccaagaggta tggctatcag gtgccgtacg cggagaagat
     1081 tcgccgcgag gccgatatca tgacaatggc cgtcggccac atcgtgcacg ccgatcaggc
     1141 cgagaccatc ctcagggaag gtcgcgccga cctgatcgcg ctcgcgcgcg agatcatgca
     1201 caatccgagc tggccgatgg acgcggcgca aaagctgggc gtcgacccgc agttccgctt
     1261 ggtgccgccg gcctacgcct attggctggc gaagcgggcc aactcgggct ttgaaggcac
     1321 gccctcgacc tggagcaagg gcctggccgc cggcgcggcg gccaaggcgg cagacaaagc
     1381 ttagcgcagc agcaccaccg cgcccggcaa agtgcccggc aaaaaattga gcagcaatgg
     1441 cgcgggcaac ggctcctcga cggctttcga gcgggagggc gaagtgctat cgataggggc
     1501 agcactggca aggttcaacc ggcctgtacc ctgggggacg agatgcctct ttacggcagc
     1561 gaaacactca agcgcattcg cgatggcaag ctgacgctcg gactgggcgt ccatcacttg
     1621 cgcggctcgg cggtgccttt gcttgccaag gccgccggct atgattggct gttcatcgac
     1681 gccgagcacg gtgccatctc gacccccgaa a
//
LOCUS       DASTMT010000324         3782 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_6326626, whole genome shotgun sequence.
ACCESSION   DASTMT010000324 DASTMT010000000
VERSION     DASTMT010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3782)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3782)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3782
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6326626"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..975)
                     /gene="trpB"
                     /locus_tag="VFR00_07540"
     CDS             complement(<1..975)
                     /gene="trpB"
                     /locus_tag="VFR00_07540"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019995990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEU0159149.1"
                     /translation="MQTEIDKRNTYRSGPDERGFFGLFGGRFVAETLMPLILELERAY
                     EAAKADPSFRHELAELNQHYSGRPSPLYFAERLTQHFRDVAAASGGGGAKIYFKRDEL
                     NHTGSHKINNCLGQIQLARRMGKTRIIAETGAGQHGVAVATVCARFGLPCEIFMGAVD
                     VERQRPNVFRMKLLGAKVNAVTSGARTLKDAMNEALRDWVANVRDTYYIIGTAAGPHP
                     YPAMVRDFQCVIGNEVREQMREREGRLPDTLVACIGGGSNAIGLFHPFLDDEGVAIYG
                     VEAAGHGLEVPNGHAASMNGGRTGVLHGNRTYLLQDGDGQILEGHSISA"
     gene            complement(1055..1705)
                     /locus_tag="VFR00_07545"
     CDS             complement(1055..1705)
                     /locus_tag="VFR00_07545"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011083568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HEU0159150.1"
                     /translation="MTTKVKICGLKEELALEAALAGGADYVGFVFFAPSPRAVGLDRA
                     RPLAARARRRGAKVVALLVDPDDGLLEGVVKAAEPDILQLHGRESPARAAEIGQRWRR
                     QVMKAIPVAAPGDAALALRYAGVADLVLFDAKAPAGATRPGGNGLAFPWGLLAEVQDK
                     VTYMLSGGLAPDNVAEAIRLTGAPIVDVSSGVESRPGEKDPVLIARFLQIAKAAQP"
     gene            complement(1801..2148)
                     /locus_tag="VFR00_07550"
     CDS             complement(1801..2148)
                     /locus_tag="VFR00_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0159151.1"
                     /translation="MLRRAVWLLVALPAAIVLITLAVSNRQAVRLVLDPFRPDDPVLF
                     LVLPLYAYLFAALIIGVLLGGAAAWFAQRRWRRMARRRSAEAVRWQAEADRLLREREH
                     RFASPAQLAPARR"
     gene            complement(2300..2578)
                     /gene="ihfB"
                     /locus_tag="VFR00_07555"
     CDS             complement(2300..2578)
                     /gene="ihfB"
                     /locus_tag="VFR00_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019994932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA];
                     GO_process: GO:0075713 - establishment of integrated
                     proviral latency [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HEU0159152.1"
                     /translation="MIKSELIQKIAAANHHLYHRDIERIVNVVFNEIVEALARGDRVE
                     LRGFGAFTVKHRAPRQGRNPRTGATVSVAEKFVPFFKTGKDLRERLNG"
     gene            complement(2622..3596)
                     /gene="sppA"
                     /locus_tag="VFR00_07560"
     CDS             complement(2622..3596)
                     /gene="sppA"
                     /locus_tag="VFR00_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HEU0159153.1"
                     /translation="MALETETVLDRRRLRRRLSLWRGLAILAALFAVGLLAFSSAERV
                     GLAERSQIARISLEGIITEDREQLKLLKKLGEAKNVAAVILFVNSPGGTTTGGEALFE
                     AIRELARSKPVVAQFGTVAASAAYIVGLATDHIVARGNSITGSVGVIFQWAEVSQLLD
                     KLGVKMNEIKSGPLKANPSPFQPLDAEGKRIAEQMVAESQRWFVGLVAARRGIDPASV
                     AGLEQGRVFSGREALVNKLIDQIGGEAEVIQWLEEKRNVPKGLRVQDWKPKRDGDWGL
                     LSSLATRALHVLIGASATERLSDLISEDGALGRLRLDGLISIWQAAER"
BASE COUNT          655 a         1267 c         1225 g          635 t
ORIGIN      
        1 cgcggaaatg gaatgaccct cgagaatttg cccgtcgccg tcctgcaaaa gataggtgcg
       61 attgccgtgc agcacgccgg tgcggccgcc gttcatggag gcggcgtgcc cgtttggcac
      121 ctccagtccg tgtccggcgg cctccacacc gtagatggcc acgccctcgt catcgaggaa
      181 ggggtggaac aaaccgatcg cgttggagcc accgccaatg caagcaacga gcgtatcggg
      241 caagcgtccc tcgcgctcgc gcatctgctc gcgcacttcg ttgccgatca cgcattgaaa
      301 gtcgcgcacc atcgcgggat agggatgcgg tccggcggct gtgccgatga tgtagtaggt
      361 atcgcgcaca ttggcgaccc agtcgcgcaa ggcttcgttc atggcatcct tgagcgtgcg
      421 cgcacccgag gtgacggcgt tgaccttggc gcccaggagc ttcatgcgaa agacgttagg
      481 tctttgtcgt tccacatcca ccgcacccat gaagatctcg catggcagcc cgaagcgggc
      541 gcatacggtt gccaccgcca cgccgtgctg gccggcacct gtctcggcga tgatgcgcgt
      601 cttgcccata cgcctggcga gctggatttg gcccaggcaa ttgttgatct tgtgactgcc
      661 cgtatggttg agttcgtcgc gcttgaagta gatcttggcg cccccaccgc cggaggccgc
      721 cgcgacgtcg cgaaagtgct gcgtcagccg ctcggcgaaa tagagggggc tcggccgccc
      781 gctatagtgc tggttgagct cggccaattc gtggcggaaa ctcggatcgg ccttggccgc
      841 ctcgtaggcg cgctcgagct ccaagatcaa cggcatcagc gtttcggcga cgaagcggcc
      901 gccgaacagg ccaaaaaagc cgcgctcgtc gggcccgctg cgataggtgt tgcgcttgtc
      961 tatttctgtt tgcatctgcc ggatcgacgt ctcacctgcc aagacctata gcacgccgcg
     1021 ccgggcttgg aacggccggc gggcgcgccg cgcgctaggg ctgcgccgcc ttggcaatct
     1081 gaagaaagcg ggcgatcaac acgggatcct tttcgccggg ccggctctcg acgccggagg
     1141 agacatcgac gatgggggca cccgtcaggc gtatcgcctc ggcaacgttg tcgggagcaa
     1201 ggccccccga gagcatgtag gtgaccttgt cctgcacctc ggccaataag ccccacggga
     1261 aggcgagccc gttgcccccg ggccgcgtcg cgcctgcggg cgctttggca tcgaagagaa
     1321 cgaggtccgc gacgccggcg tagcgcaggg ccagcgccgc atcgcctggg gcggcaacgg
     1381 gaatggcctt catgacctgc cggcgccagc gctgcccaat ctcggcggcg cgcgccgggg
     1441 actcgcgacc atgcagctgg agtatgtcgg gctcggcagc cttgacgacg ccctcgagca
     1501 ggccatcgtc gggatcgacc aggagggcga ccaccttggc gccacggcgt cgcgcgcggg
     1561 ccgccagcgg ccgggctcta tcgaggccca ccgcgcgtgg gctcggcgca aaaaatacaa
     1621 agccgacgta gtccgcccca ccggccagcg ccgcctcgag tgccagctcc tccttgagcc
     1681 cacagatttt gaccttggtc gtcacggttt tatgcgccag cagcagcggt gcgccaacct
     1741 aagctcggtt ccggccattg tccaacccgc gcgccgtcgc tcggcatgga aacgccaggt
     1801 ttaacggcgc gccggagcca gttgcgcggg ggaggcgaag cgatgctcgc gttcccgcag
     1861 caggcggtct gcctccgcct gccagcggac cgcctcggcg ctgcgacgcc gcgccatgcg
     1921 ccgccagcgg cgctgggcga accaagccgc cgcacctccg aggagcacac caatgatcag
     1981 ggccgcaaac aggtaggcgt aaagcggcag caccaggaag aggacgggat cgtcgggccg
     2041 gaagggatcc aaaaccaggc gcaccgcctg tcggttgctc accgccagcg tgatgagcac
     2101 gatggcggcc ggcaaagcca ccagcaacca aacggcccga cgcaacacgc ctcagcctcc
     2161 ctgtccgctt aagatctgag ctcgctgcgc tcccaagggc tggcaggggg gcgcaatgca
     2221 agccttcgcg cccttgtacg ccccggcgct ggcattgccg ggcggcgcgc cgatcctggc
     2281 gccggcagag ctcgcaggct cagccgttga gccgttcgcg caggtctttg cccgttttga
     2341 agaagggtac aaacttttcg gccaccgaga cggtggctcc cgtgcgcgga ttgcgcccct
     2401 gccgcggcgc gcggtgcttg accgtgaacg ccccaaaacc cctgagttcg acccgatccc
     2461 cccgcgccaa ggcctcgacg atctcgttga aaacgacgtt aacgatgcgc tcgatgtcgc
     2521 ggtgatagag atggtgatta gccgccgcga ttttttgaat gagctcggac ttaatcattg
     2581 gttctgcctc cccgccacta tacaaaagtc tttgtaatta ctcatctttc cgcagcctgc
     2641 caaatggaga tgagtccgtc aaggcgcaat ctgccgagcg ctccgtcctc gctgatgagg
     2701 tcgctgaggc gctcggtggc ggacgcaccg atcaggacgt gcagcgcacg cgtggccaaa
     2761 gagctgagca aaccccagtc gccgtcgcgt ttcggcttcc agtcctgcac ccttaggccc
     2821 ttaggaacat tgcgtttctc ttccagccac tggatgacct ctgcctcccc gccgatctga
     2881 tcgatgagct tgttgacgag cgcctcgcga ccggaaaaca cccggccttg ctcgagcccg
     2941 gcgacgctgg cggggtcgat gccacgccgc gccgccacca gaccgacaaa ccagcgttgc
     3001 gattctgcaa ccatttgttc ggcaattctc ttgccttccg catcaagcgg ttggaagggc
     3061 gagggattgg ccttgagggg gccgctcttg atctcgttca tcttcacgcc gagcttgtcc
     3121 agcaactggg agacttcagc ccattgaaag atgacgccca ccgatccggt gatgctgttg
     3181 ccgcgcgcga cgatatgatc ggtggcgagg ccgacgatgt aggcggcgga ggcggccacg
     3241 gtgccgaact gggcgaccac gggcttgctc cgggcgagtt cgcgaatggc ctcaaacagc
     3301 gcctcgccac cggtggtggt tcctccgggg gagttcacga acaggatgac ggcggcgacg
     3361 tttttagcct cgcccagctt tttcaagagc ttgagctgct cgcgatcctc cgtgatgatc
     3421 ccctcgaggc tgatacgcgc gatctggctg cgttcggcca aaccgacgcg ctcggcggag
     3481 gaaaaggcca gcaacccgac ggcgaagagc gcggccagga tcgccagccc ccgccacagc
     3541 gacaggcgcc gccgcaggcg gcggcgatcg agcacagtct ccgtttcaag cgccatgcga
     3601 gctcatgtct cttgccgggg ttgggcagct taagctgccg cagggcatgc ttcaatcaat
     3661 cgagcggcca gccgaacgcc gaacgccccg gaacggcagc ggcctcggtc tgaggcgccg
     3721 ccgcgccgcc cggccggggg gccccgctgc cagggcggga gcccggaacc gcccggttgc
     3781 ct
//
LOCUS       DASTMT010000325         4097 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_2472992, whole genome shotgun sequence.
ACCESSION   DASTMT010000325 DASTMT010000000
VERSION     DASTMT010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4097
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2472992"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            93..1298
                     /locus_tag="VFR00_07565"
     CDS             93..1298
                     /locus_tag="VFR00_07565"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0159154.1"
                     /translation="MSSATATDPGATPSRAILLLALASFVGAANLRVCDALLPQIAAE
                     LGITIGSAASMVTAFAVAYGVCQIIVGPLGDARGKLLLVVLGSLWAGVATLLCALMPS
                     LAPLVLFRFLAGAGAAAVIPLAIAWIGDVVPYERRQALLARYISGQILGIVFGQAAGG
                     LFGELIGWRATLLLLGSAHIGAGLLLLNEQRRLRAGLTSPRQPRWIEAARAAYRLVRL
                     PWVRVVLISVFIEGTAMFGALAYVGADLHARFGLGLGMVGAFLAAFGAGALLFAASAS
                     WLVPKLGQPRLAATGTLLLAGGYATLAGMPWLWLAAPAIAGLGLGFYMLHNTLQVNAT
                     QMAPQARGLAVSLFAFFLFTGQSIGVALGAPVVDRFGARPVFATAAIVLLSIGFWFRA
                     QLIKHTPAK"
     gene            1334..1978
                     /locus_tag="VFR00_07570"
     CDS             1334..1978
                     /locus_tag="VFR00_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEU0159155.1"
                     /translation="MSWSRQGRGGRRGYHHGNLREALIGAALDLISQKGPAGFTFAEA
                     ARQAGVSPAAPYRHFRDRDALMADVARQGFERFAERLAQAWDEGRPSPMLALDRLGRA
                     YLRFAREEPAYFSAMFESGVPVAKHRDLHEASERAFAVLRQACEGVVAALPQVKRPPA
                     LMMALHIWALSHGIASLFARGDAARRSLPISAEELLEAATLLYLQGLGIAGPGK"
     gene            2074..3048
                     /locus_tag="VFR00_07575"
     CDS             2074..3048
                     /locus_tag="VFR00_07575"
                     /inference="COORDINATES: protein motif:HMM:NF040043.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6282 family protein"
                     /protein_id="HEU0159156.1"
                     /translation="MQKIALQLAVLAAIGLGSDQAAAQAGFPPPPPKVSPAAGVIDMH
                     AHSHPDVFGRNLDDIDLAQLAKARGMRGIVLKNHISETASRAALVMKVVPGVEVFGGI
                     VLNRAVGGVNPDAVEWMHRIYGSRGKVVWLPTFESDKHVKTLSKPDAVGLTVAPNGQV
                     SPEMEAILKIIARENLVLATGHVHPEEIIAVVKRGRELGVKNIVVTHALTNVPGLSME
                     QAKQVAEMGAVIEICFLQFLAGPNAPLAFLTHWTQVNAKHVAQAIKELGAERVLVSTD
                     LGQSSNMVHPDGVEAAIAAMKKEGIADADIDTMMRKNPARLLGLKAEG"
     gene            complement(3095..3886)
                     /locus_tag="VFR00_07580"
     CDS             complement(3095..3886)
                     /locus_tag="VFR00_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018323066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEU0159157.1"
                     /translation="MQNRVDDDLRYEVRDGIGLVTFNRPAALNALTFEMYDRLGEICG
                     EVRLGGAVKAIIITGAGGRAFGAGTDISLFQNFATAQEGIDYEARMERMFAKLERCPV
                     PTIAAIAGVCTGGAAVIAAACDLRIASRQLKYGFPIARTLANCLSAANIARVALLTGV
                     GRALDLLLTTRLIGADEALAIGLVNELCDTPEALLERARALGAQMATQAPLTMLAGKE
                     VVRRMRANFAGIDDRDLIELCYGSRDFREGLSAFLAKRAPKFTAQ"
     gene            complement(3939..>4097)
                     /locus_tag="VFR00_07585"
     CDS             complement(3939..>4097)
                     /locus_tag="VFR00_07585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013167329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--glyoxylate aminotransferase"
                     /protein_id="HEU0159158.1"
                     /translation="RIGHLGDTNDLTLLGALAGVEMGLALAKVPHTPGGVRAAMDYFT
                     EMAATARA"
BASE COUNT          638 a         1352 c         1355 g          752 t
ORIGIN      
        1 agggcttttt tgaaactcta gcaagcaaca cgccgcgggc gtcaagcaga cttccccgcg
       61 cgccatcacg tctgagcggt gcgggcaaga gcatgagttc ggcgacagcc accgatcccg
      121 gggcaacacc ctcgcgtgcc attctgcttc tcgcgctcgc ctcttttgtc ggcgccgcca
      181 atttgcgcgt ctgcgacgcg ctgctgccgc aaatcgccgc cgagctcggc atcacgatcg
      241 gctcagccgc gagcatggtc actgccttcg ccgtggccta tggcgtctgc cagatcatcg
      301 tcggcccgct cggggacgcc cgtggcaagc ttttgctggt ggtgctgggc tcgctctggg
      361 cgggggttgc taccctctta tgcgccctca tgccctcctt ggcaccgctg gttctctttc
      421 gctttctcgc tggcgcgggc gccgcggcgg tcatcccgct ggcgattgcc tggatcggcg
      481 acgtggtgcc ttacgagcgc cggcaggcct tgctggcgcg ctacatttcc ggtcagatcc
      541 tcggcatcgt cttcggtcag gcggccggcg ggctgttcgg tgaactgatc ggctggcgcg
      601 ctaccctgct gctgctcggc agcgcgcata tcggcgccgg cctgctgctg ctgaacgagc
      661 agcgccggct gcgtgccggg ctcacttccc cgcgccagcc gcgctggatc gaggcggccc
      721 gcgcggccta tcggcttgtg cgcctgccct gggtgcgcgt cgtgctgatc agcgtcttca
      781 tcgaaggcac ggcaatgttc ggagcgctgg cctatgtggg ggccgacctg catgctcgct
      841 tcggcctcgg cctcggcatg gtcggtgcct tcttggccgc gtttggcgcg ggcgccctgc
      901 tgttcgcggc aagtgcaagc tggctggtgc cgaagctcgg ccaaccgcgt cttgccgcca
      961 cgggcaccct cctgctggcc ggcggttatg ccacattggc cggcatgcca tggctgtggc
     1021 tcgccgcccc cgccatcgcc ggcctcggcc tcggcttcta tatgctgcac aacaccctgc
     1081 aggtgaatgc gacgcaaatg gccccgcaag cgcgtggcct ggcggtgtcg ctgttcgcct
     1141 tttttctgtt tacgggccag tcgatcggcg ttgccctggg tgcgccagtg gtcgaccgct
     1201 tcggcgcccg ccccgtgttc gcaacggcgg ccatcgtcct gctatcgatc ggcttctggt
     1261 tccgcgcgca actgatcaag catacgcccg cgaagtgatg tgcccaggcg ccgaatgcgc
     1321 taaacctggc gccatgagct ggtccagaca aggccgcggc gggcgccgcg gctaccatca
     1381 cggcaacttg cgcgaggcgc tgatcggcgc tgcccttgac ctcatttcgc aaaagggtcc
     1441 cgctggtttc acgtttgccg aagcggcccg gcaggccggc gtcagcccgg cggcgcccta
     1501 tcggcatttc cgcgaccgtg atgccttgat ggcggatgtg gcgcgccagg gctttgaacg
     1561 ctttgccgaa cgcctggcgc aggcctggga tgagggccgg ccaagcccga tgctggcgct
     1621 tgaccgcctc ggccgtgcct atctacgttt cgcgcgcgaa gagccggcct acttctcggc
     1681 tatgttcgaa tcgggtgtgc ccgtggcaaa gcatcgggac ctgcacgagg cgagcgaacg
     1741 ggcctttgcc gtgctgcggc aggcctgcga gggggttgtc gccgcgctgc cccaggtcaa
     1801 acggccaccg gcgttgatga tggcgctgca catctgggcc ctgtcgcatg ggatcgccag
     1861 cctgtttgcc cgcggcgacg cggcgcgccg cagtttgccg atcagcgccg aggagctctt
     1921 ggaagcggcg acgctgctct atttgcaggg attgggtatt gccgggcccg gcaagtaggc
     1981 cgccgctgcc atttcgccgc agctcatttc tgctgtaggc ttccgagcca aggcgtcgtg
     2041 cggatcaatc gcgcgccaac ccgggaggaa accatgcaaa aaatcgccct tcagcttgca
     2101 gttttggccg cgatcggttt ggggtcggac caagccgccg cccaggcggg cttcccgccg
     2161 ccgccgccga aggtcagccc ggccgccggc gtcatcgaca tgcacgcgca ttcccatccc
     2221 gacgttttcg ggcgcaacct ggacgacatc gatctcgccc aactcgccaa ggcgcgtggc
     2281 atgcgcggca tcgtgctgaa gaaccacatc tccgaaacgg caagccgggc ggcattggtc
     2341 atgaaagtgg tgccgggggt cgaagtcttc ggtggcatcg tgctgaacag ggcggttggt
     2401 ggcgtcaatc cggacgccgt ggaatggatg caccgcatct acggcagtcg cggcaaggtg
     2461 gtgtggctgc cgaccttcga gagcgataaa cacgtcaaaa cgctctccaa gcccgatgcc
     2521 gtcggtctca ccgtggcgcc caacggtcaa gtgtcgccgg aaatggaagc gatcctcaag
     2581 atcatcgcac gcgagaacct agtgctggcg accggccacg tgcacccgga agagatcatc
     2641 gccgtagtga agaggggacg cgagcttggc gtcaagaaca tcgtcgtcac gcacgcctta
     2701 accaatgttc ccggcctcag catggaacag gccaagcaag tggcggagat gggcgctgtc
     2761 atcgaaatct gctttctgca gtttctggcc ggcccgaacg cgccgctcgc cttcctcacg
     2821 cattggacgc aggtcaatgc caaacacgtg gcccaggcca tcaaggagtt gggggcagag
     2881 cgcgtgctcg tctccactga cctcggtcaa agcagcaaca tggtgcaccc ggacggtgtg
     2941 gaggcagcca tcgccgcgat gaagaaggag ggtatcgccg acgccgatat cgataccatg
     3001 atgcgcaaga accccgcgcg gctgcttgga ctgaaggcgg agggctgagg cacgatcgaa
     3061 gacgccgggg gccaggttgt gcgctttgga gtgcctattg cgccgtgaat ttgggcgcgc
     3121 gcttggccag gaatgcgctg agcccttcac ggaagtcccg actgccgtag cagagctcaa
     3181 tcagatcgcg gtcatcgatg ccggcgaagt tggcgcgcat ccggcgcaca acttctttgc
     3241 ccgccagcat cgtgagcggc gcctgggtag ccatctgagc gcccagggcg cgggcgcgct
     3301 cgagcagcgc ctccggcgtg tcgcatagct cgttgacgag cccgatcgcc aaggcctcat
     3361 cggcaccgat caggcgcgtg gtgagcaaca agtcgagggc gcggccgacc ccggtcagca
     3421 acgccacgcg ggcgatgttg gccgccgata gacagttggc caacgtgcgc gcaatgggaa
     3481 agccatattt cagctggcgg ctggcgatcc gcaaatcgca cgccgcggcg atgaccgcgg
     3541 cgccccccgt gcacaccccc gcaatggcgg cgatggtcgg tactggacac cgctcaagct
     3601 tggcaaacat gcgctccatg cgtgcctcgt aatctattcc ctcctgcgcc gtggcgaaat
     3661 tctggaatag cgaaatgtcg gtgccggccc cgaacgcgcg tcctcccgcg ccggtgatga
     3721 tgatggcctt cacggcgccg ccaaggcgga cttcgccgca gatttcgccc agccgatcgt
     3781 acatttcgaa ggtcagggcg ttgagggcgg cggggcggtt gaaggtgacg aggccgatcc
     3841 catccctcac ctcatagcgg agatcatcgt cgacacggtt ctgcatcgtg agcctttgtg
     3901 tctcgttttt tgctggcaag ccatggccag gcgcgggctt aggcccgcgc cgtcgcggcc
     3961 atctcggtga aatagtccat cgccgcgcgc acgccgcccg gcgtgtgtgg caccttggca
     4021 agggcaagcc ccatttccac cccggcaagc gcgccgagca gggtaaggtc gttggtgtcg
     4081 ccgaggtgcc cgatgcg
//
LOCUS       DASTMT010000326         4410 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_3135798, whole genome shotgun sequence.
ACCESSION   DASTMT010000326 DASTMT010000000
VERSION     DASTMT010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4410)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4410)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4410
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3135798"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..523)
                     /locus_tag="VFR00_07590"
     CDS             complement(<1..523)
                     /locus_tag="VFR00_07590"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014491700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEU0159159.1"
                     /translation="MNFDFSDDLKALREQARKFLRERCPTAAVRRVLEGPQPYDLALW
                     QEIAAMGWTGAAIPEALGGAGLGHLGLCVLAEELGSALAPTPFSSSIYLAAEAVMLAG
                     SEAQKQAWLPKLASGAVIGTLAMAEGPGQAEPRTLRTTWRGGTLSGEKLPVPDGDVAD
                     LAVVAARGERGLQL"
     gene            complement(556..2682)
                     /locus_tag="VFR00_07595"
     CDS             complement(556..2682)
                     /locus_tag="VFR00_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006201165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HEU0159160.1"
                     /translation="MANEAADKPVVRFEIEDGIGVVIIDNPPVNALGPGVRDGIIEAL
                     EAGDADPAVKAMVMIGAGRTFIAGADIRQFGKPRPAPKRPVPDVLDQSSKPVVAAIHG
                     FALGGGLEHALACHYRIAVPSAKLGLPEVLIGILPGGGGTQRLPRLIGPKRALEMILS
                     GRHVPAEEAKTLGIIDAIVPGKDLRAEAIAYARAIVDRRPLPRVRDKTDRLEEARADP
                     GMFEATRKAIARKAGKQKAPQHCILCVEAAVSQPFDAGIATERRLFAELENSEEAKAL
                     RYAFFAEREVAKIPGLPKDLALPEIKTAAVVGAGTMGGGIAMSFADHGFAVKLLDASA
                     EVVAKGLQRIRNTYATSVRRGSLTQAEMDRRLALIEPVESYDAIAQCDAVIEAVFEEM
                     PVKQEVFAKLDAVMKPGALLFSNTSALDIDAIAGVTGRPEAVAGTHFFVPANVMKTFE
                     VVNGAKTAPVTLAAAMKLGRDIGKISAYAGNCDGFVANRTRIPFNLEQGLMIEEGALP
                     EEVDRVMVAFGYPVGPFAVNDMSGLDISYATRKRRAAADPYYRGLPITDRLVEMGRLG
                     QKTGAGWYRYQANDRTPIVDAEVHTIIKTVVGELGIGQRSFTDEEILHRLLFSAVNEA
                     CKILEEGKALRASDIDVMWLNGFGFPRYRGGLMYWADQIGARQVYNQVAIWHQRYGAR
                     WRPSELLQQVAESGGLLRETSGRIAT"
     gene            3234..>4410
                     /locus_tag="VFR00_07600"
     CDS             3234..>4410
                     /locus_tag="VFR00_07600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0159161.1"
                     /translation="MHRVRGAGRDRQALRRILNLSLYLGLAALVVASEASAQSLTGGP
                     INAATGRSLADAVTQNEQALIEREILGGSAGGGSVSGALGTFSTGRLRGSDHDALRAP
                     GLTQSFSFDTTEASAFANVVVSLPGTVLGGQMKFSGFVGHNDVSLDLKSNALSVLDAN
                     QSGSAENQSMLFGGTALWSLRNTYALATLVGTWGQTTLHDSVDDCGYGPPHPTGCNHN
                     RYTFGTAGILATVTAGQIFDLAGPAGPKLDLRGSLGYVHNSGERFKNVFGNEQTYTFS
                     TWTGTAAATLFSNMALADGAILRPYIQGYVRQEWGYRSGFDFVADPDSGRVRLQQDHL
                     YGGVDLGLTYTQGNTTFGASLYFEASGDDQTLGGRLGLSQKLDGLAQQVRPFTWSGFY
                     "
BASE COUNT          705 a         1516 c         1410 g          779 t
ORIGIN      
        1 ccagctgcag gccgcgttcg ccccgcgccg ccaccaccgc caggtcggcc acgtcgccat
       61 cgggcaccgg cagcttctct ccgctgagag tgccgccacg ccatgtcgtg cgcaacgtgc
      121 ggggctcggc ctggcccggc ccttcggcca tcgccagcgt gccaatgacc gcgccgctgg
      181 cgagcttcgg cagccaggcc tgcttctgcg cctcgctgcc cgcaagcatg acggcttccg
      241 ccgccaggta gatggaggag gagaagggtg tcggcgccag ggccgagccc agttcctcag
      301 ccagcacaca cagtccgaga tggccgagcc ccgcgccgcc caaggcctcc ggaatcgccg
      361 cgccggtcca gcccatggcg gcaatctcct gccacagggc gaggtcgtag ggctgaggtc
      421 cctccagcac gcgccgcacc gccgcggtcg ggcagcgctc gcgcaggaac ttgcgcgcct
      481 gctcgcgcag cgccttcagg tcatcgctga aatcaaaatt catgcggtcg cccctccaag
      541 ccgtcagcgc gcccctcagg tcgcgatgcg gccgctggtc tcgcgcagca agccaccgct
      601 ctcggccacc tgctgcaata gctccgaagg ccgccagcgc gcgccatagc gctgatgcca
      661 gatcgccacc tggttataaa cctggcgggc gccgatctgg tctgcccaat acatcaagcc
      721 gccccggtag cgcggaaagc cgaagccgtt gagccacatc acgtcgatgt cgctggcgcg
      781 cagcgccttg ccttcctcga gaatcttgca ggcttcgttg acggcagaaa acagcaagcg
      841 gtgcaggatc tcctcgtccg tgaagctcct ttggccgata cccaattcgc caaccacggt
      901 cttgatgatg gtgtgcactt cggcatcgac gatcggcgtg cgatcgttgg cctggtagcg
      961 ataccagccg gcgcccgtct tctggccaag tcgtcccatc tccaccagcc gatcggtgat
     1021 cggcaggccg cgatagtagg gatcggccgc ggcgcggcgc ttgcgcgtgg cgtagctgat
     1081 atccaggccc gacatatcgt tgaccgcaaa aggccccacg ggatagccga acgcgaccat
     1141 gaccctgtcg acctcttcgg gcagcgcccc ctcctcgatc atgaggccct gctccagatt
     1201 gaagggaatg cgtgtgcgat tggcgacgaa gccgtcgcaa ttgccggcat aagcgctgat
     1261 cttgccgatg tcgcgtccaa gcttcatggc ggcggccaag gtgaccggcg cggttttggc
     1321 cccgttcacc acctcaaagg tcttcattac gttggcgggc acgaaaaagt gcgtcccggc
     1381 gacggcctcg ggccggcctg tgacgccggc gatggcatcg atgtccaagg ccgacgtgtt
     1441 cgagaacagc agggcgccag gtttcatgac agcatcgagc ttggcgaaca cctcctgctt
     1501 gaccggcatc tcttcgaaca cggcctcgat cacggcatcg cactgggcga tggcgtcata
     1561 gctctcgacc ggctcgatca gggccaagcg tcggtccatt tcggcctgcg tcaggctgcc
     1621 ccgcctgacg ctcgtcgcat aggtattgcg gatgcgctgc aagcctttgg ccaccacctc
     1681 ggcggaggcg tcgagcagct tcacggcaaa gccgtggtcg gcaaagctca tggcgatgcc
     1741 gccgcccatg gtgcccgcgc cgacgacggc ggcggttttg atctcgggaa gggcgaggtc
     1801 cttcggcaaa cccggaatct tggcgacctc gcgctccgcg aagaaggcat agcggagcgc
     1861 cttggcttcc tccgagttct ccaattccgc gaacagccgt cgttccgtgg cgatgccagc
     1921 atcgaagggc tggctcacgg cggcctcgac gcaaaggatg caatgctgcg gggccttctg
     1981 tttgccggcc ttgcgcgcga tcgccttgcg cgtggcctcg aacatgcctg gatcggccct
     2041 tgcctcctcc agccgatccg tcttgtcgcg cacgcgcggc agcggtcgcc gatccacgat
     2101 ggcccgtgca taggcaatgg cttcggcacg cagatccttg cccgggacga tcgcgtcgat
     2161 gatgccgagc gttttggcct cctcggcggg cacatgccgc cccgagagga tcatctccaa
     2221 tgcccgcttc ggcccaatca ggcgcggcaa gcgctgcgtg ccgccgcccc ccggcaggat
     2281 accgatcagc acctccggca gtcccagctt cgcgcttggc acggcgatgc ggtagtggca
     2341 ggcgagcgca tgctccagcc cgcctcccag cgcaaaccca tggattgccg ccaccaccgg
     2401 tttggagctc tgatcgagca cgtcgggcac gggacgcttg ggcgccgggc gcggcttgcc
     2461 aaattggcga atgtcggccc cggcgatgaa ggtgcgaccg gcaccgatca tcaccatggc
     2521 tttgacggca ggatcggcgt cgcccgcttc cagcgcctcg ataatgccat cgcggacgcc
     2581 gggccccagc gcgttgaccg gtggattgtc gatgatgacg acaccaatgc cgtcctcgat
     2641 ctcaaaccgc accactggct tgtcggcggc ctcattcgcc atggcgtgct actcccaatg
     2701 acttcccaag tgttgctggc ccaagtgttg ctggcccaag tgttgctggc tccgcgcccc
     2761 agtgtgtttg gcggcgatcg ttcctgcaag ccgcctccat cttggccgtc ggtcgggcaa
     2821 acaatatggc ctgggcgcgc cggccgtgcg gattggattt gccgcagctt gcgccgactt
     2881 ggcgctgacc atccccagca ccggcagcgt tccgacggcg gtcggcgggg cataggggac
     2941 ggcggccggg gcgtgggccg gaggccggga gcggcgcagc cctgtggcca gctcggttgc
     3001 cgggcttgca catcagttct tccgctggcg gcgtgaaggg acgacctggg gacacgctaa
     3061 ctgcctccaa cggcgatgct ttccccgcca gccgcagccc cgaggcgccc gcgtcgcggt
     3121 tgcgctgccg caacagtcgc cgccgcaatc gtccgtgccc ggcccgtgcg aggtttcgcc
     3181 ggcggctggg cttacgctag ccttgggacg gggtagcgtc gatggagcgt acaatgcacc
     3241 gcgtgcgggg tgctggccgg gatcgccagg cgttgcgaag gatcctcaac ctcagccttt
     3301 accttggtct ggcggcgctg gttgtcgcct ccgaggccag cgcgcagagc ctcacgggcg
     3361 gcccgatcaa tgcggccacg ggccgatcgc tggccgatgc cgtaacgcag aacgagcagg
     3421 ccttgatcga gcgcgagatc ctgggtggat ccgcgggcgg agggtcggtc tccggggcgc
     3481 tcggcacgtt ctcgaccggc agattgcgcg gatccgatca tgacgccctg cgcgcgccgg
     3541 ggctgacgca atcgttctcc ttcgacacca cggaagcctc cgctttcgcc aacgtcgttg
     3601 tcagcctgcc gggcaccgtg ctcggcggac aaatgaagtt cagcggcttc gttggccaca
     3661 acgatgtctc gctcgatctc aagtccaatg cgctctctgt cctcgatgcc aatcagtcgg
     3721 gcagcgccga aaaccagtcg atgctctttg gcggcacggc gctctggtcg ctgcgcaaca
     3781 cctacgcgct ggcgacgctg gtggggactt ggggacagac gaccttgcac gatagcgtcg
     3841 acgattgcgg ctatggtcca ccgcacccca ccggctgcaa ccacaaccgc tacaccttcg
     3901 gcaccgcggg aattctcgcc accgtgacgg cggggcagat tttcgatttg gccgggccgg
     3961 ccgggcccaa gctcgatctg cgcggctcgc tcggctatgt gcacaacagc ggcgaacgct
     4021 tcaaaaacgt gttcggcaac gagcaaacct atacgttttc gacgtggacc gggaccgccg
     4081 ccgcgaccct cttcagcaat atggcgttag cggatggtgc catcctgcgt ccctacatcc
     4141 aaggctacgt gcgccaggaa tggggttatc gcagcggctt tgatttcgtt gccgatcccg
     4201 actccggccg ggtccgcctg cagcaggatc atctctatgg cggtgtcgac ctcggtctga
     4261 cctacaccca ggggaatacg accttcggcg cctcgcttta cttcgaagcc tccggcgatg
     4321 atcaaacgct gggcggccgt ctgggtctca gccagaagtt ggacgggctg gctcagcaag
     4381 tcaggccttt cacctggtcc gggttctatc
//
LOCUS       DASTMT010000327         4449 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_3463498, whole genome shotgun sequence.
ACCESSION   DASTMT010000327 DASTMT010000000
VERSION     DASTMT010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4449)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4449)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4449
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3463498"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..550
                     /locus_tag="VFR00_07605"
     CDS             <1..550
                     /locus_tag="VFR00_07605"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0159162.1"
                     /translation="HGTQPLAMFKYACFKQGIDFKDVIAVDAGSTDKMIAAFRNGEGD
                     FVHLQGPQPQQLVNDGVGHIVAALADAIGPCAFSSLAAKREWLTTDAAKAFMRAYRRA
                     RAWLISTPAAEVAAAEASYFKDIDLPVLTSTVATYQKLGNWTPHVEITRPAFAATLDI
                     FQHAGLISKRHAYEDVVAQPPA"
     gene            563..1426
                     /locus_tag="VFR00_07610"
     CDS             563..1426
                     /locus_tag="VFR00_07610"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU0159163.1"
                     /translation="MAETIGWIGVGSMGHRMSRHLVAAGHALVVADAVSTEKAPPQAR
                     VAGSNAEVAALADLIILSLPDGSVSELVARELAAASPRRVKTVIDTSTIGIEAAEAVD
                     ALLTRAGIQFIDAPVSGGTSGADKATLAIMLACPSESYARLKPLLALMGKPFHVGPKP
                     GQGQAVKLLNNFLSATALAATCEAIAFGTAQGIEMKTILDIVNASSGRNSATDDKFPR
                     RIMLGKYDAGFAAKLQLKDVRLYLDNARAAGIANEVASSVVDVWDRMNAELPGADITQ
                     MYPYTVSGRRA"
     gene            complement(1519..2469)
                     /locus_tag="VFR00_07615"
     CDS             complement(1519..2469)
                     /locus_tag="VFR00_07615"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEU0159164.1"
                     /translation="MSVAVGLGLMEFPFRDVAAYWAWVDMCEAGGVDSLWQTDRIISS
                     EPILECMVSMAALAARTRRLKFGVNVVSLALRDPVLVAKQCATIDVLSHGRLLPAFGI
                     GSPRAPEWAALHLDTGTRGRKTDEGLEIIGRLWREDSVDFAGAHYHLTGASISPKPVQ
                     AELPMWIGGSSAAAIRRTARYGTGWQAGPETPAEARGIVAAIRAASAELGRPIEDDHY
                     GAGFPFYFGQARGPQLERALDAYRRRTGRAAEGYFAIGNADTILGRIGEYVEAGVSKF
                     VLRPLGADGSEIIEQTRRLIAEVLPRVAARWPRPAKAART"
     gene            2746..3294
                     /locus_tag="VFR00_07620"
     CDS             2746..3294
                     /locus_tag="VFR00_07620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0159165.1"
                     /translation="MKSPMALLLGMSVIAPAFAQTPLGAPAPAMASVRYAPAAQRLGD
                     GQFRAEVRRAGAAADPDEVAWSSAPHATLTEALREACKMIETVYAPGTRCPLPASPAK
                     KTAQPAPAAKSAPKAASGNATTAKLGAVSPVSGYEVRGCAFYSLVNGRVVQRCPSEGA
                     PGTKPFWYNEDAFEGGYGGWGR"
     gene            3562..4131
                     /locus_tag="VFR00_07625"
     CDS             3562..4131
                     /locus_tag="VFR00_07625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0159166.1"
                     /translation="MIFFHGFAIDSGIAGVATAMMALAGSAWMVQYKTRAEEQPFEKY
                     VADAAVAMRDEAYVLMKMAELPPRPEPAAEARTLGDQVERLRRCLKVFQKGSPYTQIR
                     NYGARMRIEHLEEEVSEQVGLLDREIAILGTYPTKNVVQVAKQKCRRASEGILASTQQ
                     LCEVLGYEALLPSEAEVLTRTGGLSAAQV"
     gene            complement(4232..>4449)
                     /locus_tag="VFR00_07630"
     CDS             complement(4232..>4449)
                     /locus_tag="VFR00_07630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEU0159167.1"
                     /translation="RPPLHFRRKAMLEQHCRGWALGELNAALACLNAAAKAARLASSI
                     EATLAQKLIIELAAIGPQGRAPGEARP"
BASE COUNT          768 a         1481 c         1484 g          716 t
ORIGIN      
        1 ccacggcacc cagccgctgg ccatgttcaa gtacgcctgc ttcaagcaag gcatcgactt
       61 caaggatgtg attgccgtcg atgccggctc gacggacaag atgatcgccg cctttcgcaa
      121 cggcgagggc gatttcgtac atctgcaagg tccccaaccg cagcaactgg tcaacgatgg
      181 cgtcggccac atcgtggccg cgctcgcgga cgcgatcggg ccgtgcgcgt tttcgagcct
      241 ggccgccaag cgtgaatggc tgacaaccga cgccgccaag gccttcatgc gcgcctaccg
      301 cagggcgcgg gcctggctta tcagcacgcc tgcggccgag gtcgccgcgg cggaggcctc
      361 ctatttcaag gacatcgacc tgccggtgct gacctccacc gttgccacct atcagaagct
      421 cggcaattgg acgccgcacg tggaaattac gcgtcccgcc tttgccgcca cactcgacat
      481 cttccagcat gcgggcctta tcagcaaacg gcacgcctac gaagacgtcg tcgcgcaacc
      541 cccggcttga ggagccaaga ccatggcaga gacaatcggc tggatcggcg tcggcagtat
      601 gggccaccgc atgagccggc acctggtggc agccggccac gccctggtgg tggccgacgc
      661 ggtcagcacc gaaaaagcac ctccgcaggc gcgcgtcgct ggcagcaacg cggaagtggc
      721 cgcgcttgcc gacctgatca ttctatcgct gcccgacggc agcgtgagcg agctggttgc
      781 acgcgagctg gcggcggcct cgccgcgccg ggtcaagacg gtgatcgaca cttcgaccat
      841 cggtatcgaa gctgccgaag ctgtcgacgc gctgctcacc agggccggca tccagtttat
      901 cgacgccccg gtctcgggcg gcacgtcggg agccgacaag gcgacgcttg ccatcatgct
      961 ggcttgcccg agcgagagct acgcgcggct gaaaccactg ctggcgctga tgggcaagcc
     1021 cttccacgtc ggcccgaaac caggtcaagg tcaggcggtc aagctgttga acaactttct
     1081 ttccgccacc gcactcgcgg caacctgcga ggcgatcgct ttcggcaccg cgcaggggat
     1141 cgagatgaag accattctcg acatcgtcaa tgcgtccagc gggcgcaatt cggcgaccga
     1201 cgacaagttt ccccgccgca tcatgctcgg caaatatgat gccggctttg ccgccaaact
     1261 gcagttgaag gacgtgcggc tctatctgga caatgcccgg gcagcgggta tcgccaatga
     1321 ggtggcaagc agcgtcgtcg acgtgtggga caggatgaac gcggagctgc ccggcgccga
     1381 tatcacgcaa atgtatccct ataccgtgag cggtcggcgc gcctgaccgc gccgcccagc
     1441 gcaagcggtg gcgcccggca ctgtcgtccc gcgccggggc aagggtcgat ccggccgcgg
     1501 aggcaagctc gggtcggctt aggtgcgcgc ggcttttgcc ggtcgcggcc agcgtgccgc
     1561 aaccctgggc aacacctcgg cgatcagccg gcgcgtttgc tcgatgatct cgctgccatc
     1621 ggcacccagg ggccgcagca cgaatttgga gacgccagcc tcaacgtact cgccgatccg
     1681 gcccaggatg gtatcggcgt tgccgatggc gaagtagccc tccgcggcac gcccggtgcg
     1741 ccgccggtac gcgtccaacg cacgctctag ctgcggaccg cgggcttgcc cgaaatagaa
     1801 tgggaagcca gcgccatagt gatcatcctc gatcgggcgc cccagttcgg cgctggcggc
     1861 gcggatggcc gcgacgatgc cgcgcgcctc ggctggcgtt tccggacctg cctgccagcc
     1921 ggtgccgtag cgtgccgtgc gccgaatggc tgccgctgac gagcccccaa tccacatcgg
     1981 cagctccgcc tgcacgggct tgggcgagat gctggcgccc gtgagatggt aatgcgcgcc
     2041 cgcgaagtcg acgctgtcct cgcgccagag ccggccgatg atctcgaggc cctcgtcggt
     2101 cttgcgccca cgcgtgccgg tatcgaggtg aagcgcggcc cattcgggtg cgcgcggact
     2161 gccgatgccg aacgctggca acagccggcc atgcgatagc acgtcgattg tggcgcactg
     2221 tttggccacc aataccggat cgcgcagggc gagcgaaacc acattgaccc cgaacttgag
     2281 ccgccgggtc cttgccgcca gtgcggccat actcaccatg cactccagga tcggctcgct
     2341 gctgatgatg cggtcggttt gccatagaga gtcgacgcca cctgcctcgc acatatcgac
     2401 ccatgcccaa taggccgcaa catcccggaa cggaaattcc atcaagccca ggccgacggc
     2461 gactgacatt gcgatacctc gcgcttggtc gcgacccaca ctcgcggcct atccgctgag
     2521 cgtcaagcac caagcgccca aacgtagaca gacgatcggt tttttcgctg ctggcgcagg
     2581 cagatgcgca cgttcgcagt ggtgccactt tcctgcgctt cactgtcgcg gccgcgcacg
     2641 aggaagacac cgcgttttca atttctgccg cgtcgggcgg cgatgtttgc agtgccattt
     2701 tctctctttg agggaacaac ctattgcctt tttggaggaa gcctaatgaa aagcccgatg
     2761 gccttgctgc tcggcatgag cgtgattgca cccgcgttcg cccaaacgcc gctcggtgcg
     2821 ccggcgcccg ctatggcaag cgtgcgttac gcgcccgctg cgcaacgcct cggtgacggg
     2881 caatttcgcg ccgaagtccg gcgcgccggt gccgccgccg atcccgatga ggttgcctgg
     2941 agcagcgcgc cccatgcgac gcttaccgag gcgttgcgcg aagcgtgcaa gatgatcgaa
     3001 accgtctatg cgccgggcac ccgctgtcct ctgcccgcca gcccggccaa aaaaacggcg
     3061 cagccggcgc cggcggccaa gtccgcgccg aaggcagcga gcggcaacgc tacgacggca
     3121 aagctgggcg cggtttcccc cgtcagcggt tatgaggtgc gcggctgcgc cttttacagt
     3181 ctcgtcaatg gcagggtcgt gcagcgttgc ccgagcgaag gcgcgccggg caccaagccg
     3241 ttctggtaca atgaggacgc ctttgaaggc ggctacggcg gctggggacg ctgagcccga
     3301 cccgggcctg ccctgcggcg cacggcaggg tggccccccg cccgcgcggg gtggcggcga
     3361 atcggctaaa acgccaaccc tcaaaggtcc ggggcgccca gggggcgcac gcttggcgtt
     3421 gggggtggca gcatgcacgg cgccgagacg gggccaaggc gcgggacgtg gcgaggtcgc
     3481 tgcgcacggc tcctcaagtc ccgctcgctg cccttggtgc tgaccgcttg gtgcgccggc
     3541 ctcgaaatgg gctgggcttg gatgatcttt tttcacggct ttgccatcga cagcggcatc
     3601 gccggcgtcg ccacggcaat gatggcgctc gcgggttcgg cgtggatggt gcagtacaaa
     3661 acgcgtgccg aagagcagcc gttcgagaaa tatgtggccg acgccgccgt ggccatgcgc
     3721 gatgaggcct atgtgctgat gaaaatggcc gagctgccgc cacggccgga accggccgcg
     3781 gaggcacgga cgcttgggga tcaagtcgag cgcctgcgcc gctgtctcaa ggtgtttcaa
     3841 aagggaagtc cctacaccca gatcagaaat tacggggcgc gcatgcgtat cgagcacctg
     3901 gaagaagaag tcagtgaaca ggtggggctc ctggatcgag agattgccat tctcggcacc
     3961 taccccacca agaacgtcgt gcaggtcgcc aagcaaaaat gccggcgcgc cagcgaaggc
     4021 atcctggcga gcacgcaaca gctctgcgag gtcctggggt acgaggcgct gttgccgtcg
     4081 gaggcagagg tattaacgcg gaccggtggt ctcagcgccg cccaagttta agcgcgcggc
     4141 gggcgctgcg gcgcgcgcgc ggcgccgggg gcaggccgag ccgcgccggg ggatttgtcg
     4201 ccgcaacgac gccgctgact acgaggctcc gctacgggcg agcctcgccc ggcgcccgcc
     4261 cctgcggccc gattgcggcc agctcgatga tgagcttttg cgccagcgtc gcctcgatgc
     4321 tgctggcgag ccgggccgcc ttggcggcgg cattgaggca ggccagggct gcgttgagct
     4381 cgccgagcgc ccagccgcga cagtgctgct cgagcatggc cttgcgccgg aagtgtaagg
     4441 gtgggcgct
//
LOCUS       DASTMT010000328        10528 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_5671930, whole genome shotgun sequence.
ACCESSION   DASTMT010000328 DASTMT010000000
VERSION     DASTMT010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 10528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10528)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10528
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5671930"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..653
                     /locus_tag="VFR00_07635"
     CDS             <1..653
                     /locus_tag="VFR00_07635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015932492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HEU0159168.1"
                     /translation="REFVAAVLGSTEAQWKEIFAQSGQTYEAPTLVMFSGSTSSACGY
                     AQSAMGPFYCPNDRKIYLDTSFFDDLERRFRGCDVGSKACQFSQAYVITHEVGHHVQN
                     LLGILPQVQQQQRALDQADGNQLQVRVELQADCFAGVWGHYAADKWGIIEPGDVEAAI
                     QTASAIGDDRLQRRSQGYVVPDAFTHGSSAQRTRWFLTGLKSGQVGSCDTFRAEPL"
     gene            1188..2429
                     /locus_tag="VFR00_07640"
     CDS             1188..2429
                     /locus_tag="VFR00_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002712762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HEU0159169.1"
                     /translation="MTSRSLLAAFVATGLLAGSPALAQDTIKIAYIDPLSGPGATVGE
                     VGLKTFQFLAEELNAKGGAAGKKFEILPFDNKTNPQESLVQVQKAVDAGARIITQGNG
                     SSVAAALTDWVTKYNERNPGKEVLYLNYAAVDPVLTNEKCSFSHFRWDANSDIKMEAL
                     TNYMKGQKDIKKVYLINQDYSFGQAVRKAATEMLKAKRPDIQVVGDELHPLLKVTDFA
                     PYIAKIKASGADTVITGNWSQDFALLLKAAADAGLQVNWYTYYAGGTGGPTSVKQTGL
                     NHRVFAIQEGSANVDHAASQATEKAFRDKFGVSNFYPRAFNEMRMLAAAINKANSTDP
                     NKIAAALEDMKFDVFNGGEGFMRKDDHQFFQPMYIVSLGDLGPKEPFDEEKTGWGWRT
                     IARLDAKDTVLPTTCKMQRPS"
     gene            2496..3446
                     /locus_tag="VFR00_07645"
     CDS             2496..3446
                     /locus_tag="VFR00_07645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011502000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEU0159170.1"
                     /translation="MAELVLISSLNGVLYGLLLFLMASGLTLIFSMMGVLNFAHASFY
                     MLGAFLGFQISRWAGFWWALIWAPVLVGLAGALVERFGLRNVHRHGHVAELLFTFGLA
                     FVIEELVQIVWGKSPVDFRTPALLDFPAFTVFATNYPAYKLFMLVISIAIFVGLFVML
                     TRTRIGLIVQAALTHPHMVAHLGHNVPLVFMAVFGVGAALAGIAGVIAGPALVTQASM
                     AALLGPILFVVVVVGGLGSLQGALFASLLIGLLQTFAISINLSLSDIFAGSGFTPAPN
                     TLLSDLWRVSVAQIAPILPYVLLILVLIFKPTGLFGNRET"
     gene            3443..4753
                     /locus_tag="VFR00_07650"
     CDS             3443..4753
                     /locus_tag="VFR00_07650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002712764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEU0159171.1"
                     /translation="MSDAIATPAATTPAQTGIARLAPWGVWLVLAVVLAALPLLFRSG
                     TALTMLSLMGIAIIFALSYNMLLGQTGMLSFGHAVYYGLGAYFTVHAINWAIAAKLPI
                     PLLVMPLVGGFAGLVFAIVFGWVSTKRSGTAFAMISLGLAELVGSSSLILRSFFGGEE
                     GVTTNRTKLLRLFDWNFGPQIQVYYLIAGWCLLSIALMYALTRTPWGRMCNAVRDNSE
                     RAQFVGYNPQVVRYIAFCLSGLFAGVAGGLASINFELANSALFSAAQSGNVLLATFIG
                     GAGHFVGPILGAVLVTYLQNMLSDVTEVWQLYFGLMFIATVMYAPGGLAGLIMMHGPI
                     WRAGALSGLAAAYALLLAPIALVAVGAVLVIEMMTHLGVKAAEGTVMKMAGISFDARS
                     GLAWALALGLLLAGGGLALLAWRGVVAPAWDRAQAKAREKGLTL"
     gene            4750..5532
                     /locus_tag="VFR00_07655"
     CDS             4750..5532
                     /locus_tag="VFR00_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015688229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0159172.1"
                     /translation="MNGRAEAGGGARLAALEARGLRKNFGAAEIIRGVDLSVPKGERH
                     AVIGPNGAGKSTLFNLISGLLLPTAGEIRLDGAPVNGLPAYKINRRGLSRSFQVTNIF
                     PKLSVWENVRCAVLWSRGEKYSFWRSVEANPEIAQRTQQVLHEINLSSRKDLPAGVLT
                     YAEQRALEIGITMAGGANVILLDEPTAGMSHAETERAVALIRRISEGRTLLIVEHDMS
                     VVFGLADRISVLVYGEIIASGTPAEIRGNAKVREAYLGEEAR"
     gene            5532..6227
                     /locus_tag="VFR00_07660"
     CDS             5532..6227
                     /locus_tag="VFR00_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011474577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0159173.1"
                     /translation="MLEVRDLNAYYGKSHILQGVNLDVAAGEVVSLLGRNGVGRSTTV
                     KAIMGEVEPMGSIRFKGRDIAGLPSYRIARLGLGYVPENRDIFPSLTVEENLRLGQKD
                     PRRQGRWRLTDMLDMFPNLKARVGTPAGVLSGGEKQMLTMCRTLMGDPEMIMIDEPTE
                     GLAPLIVKQVGALISEIARRGLAILLVEQKLSIALDISQRVYVMGHGRIVFEGTPADL
                     KANQNVRHEWLEV"
     gene            complement(6300..7943)
                     /locus_tag="VFR00_07665"
     CDS             complement(6300..7943)
                     /locus_tag="VFR00_07665"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HEU0159174.1"
                     /translation="MVDTFDAEFDVVVAGAGSAGCVLATRLTEDPELSLCLIEAGQRD
                     RNPWIHIPLGFGKLVPNPKVNWGYETEPEPQLNGRRLSWPRGRVLGGSGSINGLVFLR
                     GAPSDYDGWAAQGATGWGYKDVLPYFKRMEHNVRGADAYRGQGGPMTISDIKNPSANA
                     RAFVEACTRLQYPLNSDFNGERIDGVGIVQLNVRNGWRCSTAVGYLKPNLGRKNLKLM
                     TESLVRRVLFEGRRAVGVEVEERGVLKRIRARREVIVSAGAINSPMLLLNSGVGPAAE
                     LSQFGIEVTHDLPGVGKNLQDHVQARFTFLTRTAGTLNEILTSKRQQARMAWEWLTAS
                     SGQLAVGATEATLFAKSSPNEPAPDLQFQSTNFSTEHLTLGLDRWPGFGFNFCVCRPK
                     SRGEIKLADGTRRSKPKIFANYFSDAEDDDMRRSVAAFHMGRQIIATEPYRSLIFCEV
                     RPGAQVTAEDEIRAFIRQVAGTVYHPCGTCRMGQDDKAVVDTELKVRGLEGLRVADAS
                     VMPIIPSPNIHPATIMIAEKGADLIARALANGRSPAIAA"
     gene            complement(8075..8506)
                     /locus_tag="VFR00_07670"
     CDS             complement(8075..8506)
                     /locus_tag="VFR00_07670"
                     /inference="COORDINATES: protein motif:HMM:NF016001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HEU0159175.1"
                     /translation="MANYIPSPRDWVREQVELYESSGGTKGTTLRDTGLPVVLVTHSG
                     NKTGAIRKTPLMRVKDGNGYVLVASQGGAPKDPVWVHNLRANPQVELRDETVVRPMRV
                     VEVKDPAERERLWKLAVAAFPPYADYQAKTSRQIPLFLATP"
     gene            complement(8656..9471)
                     /locus_tag="VFR00_07675"
     CDS             complement(8656..9471)
                     /locus_tag="VFR00_07675"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HEU0159176.1"
                     /translation="MSAQSLKRLLPALLLLLAVCAVTGRLWSAQAPIPMPDLVALPPA
                     DFLYRAAGEVLRDGQPAAPPRLLTRLERPLLIMRQQVTAAEYQRCVSAAACAGSEDAA
                     DPNTPVVKVSWRDATAYAAWLSRTTGMAFRLPTDREWAYAAGSRLADDALAESAELAD
                     PGRRLLARYDQEWDRAAGLAKAPQPTGTYGANEHGLLDVAGNVWEWTDSCFVRTRLDR
                     DGEVASEIANCGVRLVEGRHRAYVPDFIRDARAGGCSAGTPPSHLGFRLVRAS"
     gene            complement(9499..>10528)
                     /gene="nirK"
                     /locus_tag="VFR00_07680"
     CDS             complement(9499..>10528)
                     /gene="nirK"
                     /locus_tag="VFR00_07680"
                     /EC_number="1.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014492464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050421 - nitrite reductase (NO-forming)
                     activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="copper-containing nitrite reductase"
                     /protein_id="HEU0159177.1"
                     /translation="AAGDLNLPRQKVELVAPPYVHAHQQATSEGPKIMEFRLVAEEKQ
                     IVIDEEGTKLRAMTFNGSIPAPLMVVHEGDYVEVTLVNLASNTMPHNIDFHSATGASG
                     GGDLTLVAPGEQVRLRWKATRTGVFVYHCIPGGPMVPWHIAAGMSGAVMVLPRDGLKD
                     GAGKPLTYDRVYYLGEQDFYVPRDQKGKFKSYPSHVEAFADTMDLMHKLIPTHVVFNG
                     AVGALTGKNAMPAKVGETVLIIHSQADRDSRPHLVGGHGDYVWETGKFANPPQTDLET
                     WFIRGGSAGAALYKFLQPGVVAYVNHNMIEAFELGATAHFRIEGPWNEDLMSQVRAPG
                     PVQKSNLD"
BASE COUNT         1681 a         3391 c         3521 g         1935 t
ORIGIN      
        1 accgagagtt cgttgcggcg gtcctcggta gcacggaagc gcagtggaag gagatctttg
       61 cgcaaagcgg tcaaacctac gaggcaccga cgctcgtgat gttttcgggg tcgacgtcgt
      121 cggcctgcgg ctatgcccaa tcggcgatgg gcccgttcta ttgtccgaac gatcgcaaga
      181 tctatctcga cacgtcgttt ttcgatgatc tggaacgccg ttttagaggg tgcgacgtcg
      241 gcagcaaggc ttgccaattc tcacaggctt atgtgatcac ccacgaggtt ggtcatcacg
      301 tgcaaaactt gcttgggatc ctgccgcagg tgcaacaaca acagcgcgcg ctcgatcagg
      361 ccgacggcaa ccagctgcag gttcgcgtcg agctgcaggc ggattgcttt gccggggtgt
      421 ggggtcatta cgcggccgac aagtggggca tcatcgagcc cggggacgtc gaggccgcga
      481 tccagacagc gtcggcgatc ggcgacgatc gactgcagcg gcgcagccag ggctatgtgg
      541 tgcccgatgc ttttacgcat ggatcctccg cgcagcgcac gcgctggttc ctgaccggac
      601 tgaaatccgg gcaggtgggc agctgcgaca ccttccgggc ggagcccttg tgagggccgc
      661 gttctcgctg tcagggcgct gagccgcacg gcctgctgct gcagcgtggc gcccaagtcc
      721 cgaacaccca ggtctgcagc gaaagctaag gcgttcgaga cgtggtcttg ccctaggcgc
      781 gcgccgcgac gagcgtggcc acgcccggca agacccggcg gtgtttgccg ccgccatgtt
      841 cgcgcgcggc actctccctg cggtgctccg ccgagtgcag caggcgcctg gccggccctc
      901 atcttcgcga ccagcaactt gcagtcccac ggccaggggc gacagctcgc gacagctcca
      961 aggccttggc gtgagcgcgg gcacagcgag ggctcccgtc cctggtatgc caggcagtcc
     1021 gtgcacttag gctctggagc ggcgccgcgg tttgccataa gaagacagct cagtgccggt
     1081 cccttgtgcg gcgtgctccc gaaggtgagc cggcgccgcg cggccggcga gcgcggaatt
     1141 cgcgtgcgga gcaggaaaca tcccttggtt aggctttcgg aggcgtcatg acatcccgtt
     1201 cgttgcttgc ggcgtttgtt gcaacaggct tgctcgccgg ctcacctgcg ctcgcgcagg
     1261 acaccatcaa aatcgcctat atcgatcccc tttcgggccc gggtgcgacg gtcggcgagg
     1321 tcggcctcaa gaccttccag ttcctggcgg aggagctcaa cgccaagggc ggggcggcgg
     1381 gcaagaagtt cgagatcctg cccttcgaca acaagaccaa tccgcaggaa agcctggtgc
     1441 aggtgcaaaa ggccgtcgat gcgggcgcgc gcattatcac ccagggcaac ggctcctcgg
     1501 ttgccgccgc gctcaccgat tgggtcacca agtacaacga gcgcaacccc ggcaaggagg
     1561 tgctttacct caattacgcc gcggtcgatc cggtgctgac caacgagaag tgctcgttct
     1621 cgcatttccg ctgggatgcc aactccgaca tcaagatgga ggccctcacc aactacatga
     1681 agggccagaa ggacatcaaa aaagtttacc tgatcaatca ggactattcc ttcggccagg
     1741 ccgtgcgcaa ggcggcgacc gagatgctga aggccaagcg ccccgacatc caggtggtcg
     1801 gtgacgaatt gcacccgctg ctcaaggtca cggacttcgc cccctacatc gccaagatca
     1861 aggcctccgg cgccgacacg gtgatcaccg gcaactggag ccaggatttt gccttgctgc
     1921 tgaaagcggc ggccgatgcc ggcttgcagg tcaactggta cacctactat gccggcggca
     1981 cgggtgggcc gacctcggtc aagcagacgg gcctcaacca ccgagtcttt gcgatccagg
     2041 aaggctcggc caacgtcgac catgccgcct cgcaagcgac cgagaaggcg ttccgcgaca
     2101 agttcggcgt gtcgaacttc tatccgcgcg ccttcaacga gatgcggatg ctggcggccg
     2161 ccatcaacaa ggccaactcc acggatccca acaagatcgc ggcggcgctc gaggacatga
     2221 agttcgacgt cttcaacggc ggtgaaggct tcatgcgcaa ggacgaccat cagttcttcc
     2281 agcccatgta catcgtctcc ctgggtgacc tcggtcccaa ggagcccttc gacgaggaaa
     2341 agaccggctg gggctggcgc accatcgccc ggctcgatgc caaggacacc gtgctgccga
     2401 ccacctgcaa aatgcagcgt ccgagctaac acgacgcctt cgctgggccg ccgaacggca
     2461 agaagtcggc ggcccttagc ccgggagggc gcctcgtggc ggaactggtt ctgatctcga
     2521 gcctgaacgg cgtcctttac ggtcttttgc tgttcttgat ggcgagcggg ctgacgctca
     2581 tcttttccat gatgggcgtg ctcaattttg cgcatgccag cttctacatg ctgggagcct
     2641 tcctcggttt ccagatcagc cgctgggcgg gcttttggtg ggccttgatc tgggcgcccg
     2701 tgctggtggg gctcgccggt gccctggtcg agcgcttcgg gctgcgcaac gtgcaccggc
     2761 acggtcacgt cgccgagctg ctcttcacct tcggcctggc cttcgtgatc gaggaattgg
     2821 tgcagatcgt ctggggcaag tcgcccgtcg attttcgcac gccggcgctg ctcgactttc
     2881 cagccttcac ggtgtttgcc accaactacc ccgcctacaa gctttttatg ctggtgatct
     2941 cgattgccat cttcgtcggc ctgtttgtga tgctgacgcg cacgcgcatc ggtctcatcg
     3001 tgcaggcggc cctgacccac ccgcacatgg tggcccacct cggccataac gtgcccttgg
     3061 tgttcatggc ggtgttcggc gtcggcgccg cgctcgccgg catcgccggc gtcatcgccg
     3121 ggccggcgct ggtgacgcaa gcgagcatgg cggcgctgtt ggggccgatc ctgttcgtgg
     3181 tcgtggtcgt cggtggcctc ggctctctgc aaggcgcctt gtttgcctcg ctgctgatcg
     3241 gcttgctgca gacttttgcg atctccatca acctgtcgct gtccgatatc tttgccggct
     3301 ccggcttcac gcccgcgccc aacacgctgc tgagcgacct gtggcgtgtg tcggtggcgc
     3361 agatcgcgcc catcctccct tacgtgctgc tcattctcgt cttgatcttc aagcccacgg
     3421 ggctgttcgg gaaccgcgaa acatgagcga tgcgatcgca acgccggcgg ccacgacgcc
     3481 agcgcaaacg ggcattgcac ggctggcgcc gtggggcgtc tggcttgtct tggccgtggt
     3541 gctggcggcg ctgccgctgc tgtttcgctc cggcacggcg ttgacgatgc tgagcctgat
     3601 gggcatcgcc atcatctttg ccctctccta caacatgctg ctcggtcaaa cgggcatgct
     3661 gtcctttggc cacgccgtct actacgggct cggcgcctat ttcacggtgc acgccatcaa
     3721 ttgggcgatt gccgccaagc tgcccattcc cctcctggtc atgccgctcg tcggcggctt
     3781 tgccggcctc gtgttcgcca ttgtctttgg ctgggtgtcg accaagcgca gcggcaccgc
     3841 ctttgccatg atctcgctgg gcctggccga gttggtgggg tcgagctcgc tgatcctgcg
     3901 ctccttcttc ggcggcgagg aaggcgtcac gaccaaccgc accaagttgt tgcgcctgtt
     3961 tgactggaac ttcgggccgc aaatccaggt ctattatcta atcgccggct ggtgcctgct
     4021 gtcgattgcc ctcatgtatg ccctgacccg caccccctgg ggccgcatgt gcaatgccgt
     4081 gcgcgacaat tcggagcgcg cgcagttcgt cggctacaac ccgcaggtgg tccgctacat
     4141 cgccttttgc ctgtccggcc tgttcgccgg cgtcgccggc gggctagcct cgatcaattt
     4201 cgagctcgcc aattcggcgc tcttcagcgc ggcgcaatcg ggcaatgtgc tgctggcgac
     4261 cttcatcggc ggtgccggtc acttcgtcgg tcctattctg ggcgccgtac tcgtcaccta
     4321 cctgcaaaac atgctgagcg atgtcaccga ggtttggcag ctctacttcg gcttgatgtt
     4381 catcgccacg gtcatgtacg cgcctggagg ccttgccggg ctcattatga tgcacgggcc
     4441 gatttggcgg gccggcgcgc tgtcgggcct cgccgccgcc tatgccttgc tgctggcccc
     4501 gatcgcgctg gtcgcggtgg gcgccgtgct ggtgatcgag atgatgacgc accttggcgt
     4561 caaggcggcc gagggcaccg tcatgaagat ggccggcatc agctttgatg ccaggagcgg
     4621 gctcgcttgg gcgttggcgc tcggcttgct gctcgccggc ggcggcctgg cgctgctggc
     4681 ctggcgtggc gtcgtcgcgc ccgcctggga tcgggcgcag gcaaaagcgc gcgagaaggg
     4741 gctgacccta tgaacggacg cgccgaggca ggagggggtg cgcggcttgc ggcgctcgag
     4801 gcgcgcggcc tgcgcaagaa ctttggcgcc gccgagatca ttcgcggcgt cgatttgagc
     4861 gtgcccaagg gcgagcgcca cgcggtgatc ggcccgaacg gggccggcaa atcgaccttg
     4921 ttcaacctga tcagcggcct gctgctgcca acggccgggg agatccgctt ggacggcgcg
     4981 ccggtcaatg gcttgccggc ctacaagatt aaccgtcgcg ggctctcccg ctccttccag
     5041 gtcaccaaca tcttccccaa gctcagcgtt tgggaaaacg tgcgctgcgc ggtgttgtgg
     5101 tcgaggggcg agaaatattc cttctggcgc tcggtcgagg ccaacccgga gattgctcag
     5161 cgcacccagc aggtgctcca cgaaatcaac ctcagctcgc gcaaggacct gcccgcgggc
     5221 gtgttgacct atgccgagca gcgcgcgctc gagatcggca tcaccatggc gggcggcgcc
     5281 aacgtgatcc tgctcgacga gccgaccgcc ggcatgagcc atgccgagac ggagcgcgcc
     5341 gtggccttga tccgccgcat cagcgaaggg cgcacgctgc tgatcgtcga acacgatatg
     5401 agcgtcgtgt tcggcttggc cgaccgcatt tccgtgctgg tctatggcga gatcatcgcc
     5461 agcggcacgc ccgcggagat tcgcggcaac gccaaggtca gggaagccta ccttggcgag
     5521 gaggcacgct gatgctggaa gtccgcgacc tcaacgccta ttacggcaaa agccatatcc
     5581 tgcagggcgt caatctcgac gtcgccgccg gcgaggtcgt gagcttgctc ggccgcaacg
     5641 gcgtcgggcg ttccaccacg gtcaaggcca tcatgggcga ggtcgagccc atgggaagca
     5701 ttcgcttcaa gggtcgcgat atcgccgggc tgccgagcta tcgcattgct cgcttgggcc
     5761 tcggctatgt ccctgagaac cgcgacattt ttcccagcct gacggtggaa gagaacctgc
     5821 ggctcgggca aaaggatccg cggcgccagg ggcgctggcg ccttaccgac atgctcgaca
     5881 tgtttcccaa tctgaaagcc cgcgtcggca ccccggccgg cgtcctctcc ggcggcgaga
     5941 agcagatgct gaccatgtgc cgcaccctga tgggcgatcc ggaaatgatc atgatcgatg
     6001 agccgaccga aggcctggcc cccctgatcg tcaagcaggt gggcgcgctg atttccgaga
     6061 tcgcccggcg tggcctcgcc attctgctgg tggagcagaa gctgtcgatc gcgctcgata
     6121 tctcccagcg tgtctacgtc atggggcacg ggcgcatcgt cttcgagggc acgccggccg
     6181 acctcaaggc caaccaaaac gtgcgccacg aatggctgga ggtgtaagcc tccggctcgg
     6241 gccgccccct gccgctcggg cagagcgtgg cgtggctcaa gcccgagcct tggctccaat
     6301 catgccgcga tggccgggct gcggccgttg gcaagcgcgc gggcgatgag gtcggctccc
     6361 ttttcggcga tcataatggt ggcggggtgg atattgggtg aggggatgat cggcatgacc
     6421 gaggcatcgg cgacgcggag gccctcgagg ccgcgcacct tgagctcggt gtcgaccacg
     6481 gccttgtcgt cctgtcccat gcggcaggtg ccgcaggggt gatagaccgt gccggcgacc
     6541 tgccggataa aggcgcggat ctcgtcctcg gccgtgacct gggcgcccgg tctcacttcg
     6601 cagaaaatca gggaccggta gggctcggtg gcgatgatct gccggcccat gtggaaggcg
     6661 gcgacggagc ggcgcatgtc gtcgtcctcc gcatcgctga agtaattggc aaaaatcttg
     6721 ggtttggagc ggcgcgtgcc atcggcgagc ttgatctcgc cccggctctt gggccggcac
     6781 acacaaaaat tgaagccgaa gcccggccag cggtcgagcc ccaaggtaag atgctcggtc
     6841 gagaagttgg tcgactggaa ctgcagatcg ggcgccggtt cgttcgggct cgacttggca
     6901 aacagcgtcg cctcggtggc ccccacggcg agctggcccg aagaggccgt cagccactcc
     6961 caggccatgc gcgcctgctg ccgcttcgag gtcaagatct cgttcaaggt ccccgccgtc
     7021 cgggtcagga aggtgaagcg ggcctgcacg tgatcctgca ggttcttgcc cacccccggc
     7081 aggtcgtgcg tcacctcgat gccgaattgg ctgagctcgg cggccggacc gacacccgag
     7141 ttcagcaaca gcatgggcga gttgatggcg ccggcggaga cgatcacctc gcggcgcgcc
     7201 cggatccgct tcaggacgcc gcgttcctcg acttcgacgc cgacggcgcg gcggccctcg
     7261 aacaggacgc ggcgcaccag gctctcggtc atgagtttca ggtttttgcg ccccaggttc
     7321 ggcttgaggt agccgaccgc ggtggagcag cgccagccat tgcgcacgtt gagctggacg
     7381 atgccgaccc cgtcgatgcg ctcgccattg aagtcggagt tcaagggata ctgcaggcgc
     7441 gtgcacgcct ccacgaaggc gcgggcattg gccgaggggt ttttgatgtc ggagatggtc
     7501 atggggccgc cctggccgcg ataggcgtcg gcgccgcgca cgttgtgctc catgcgtttg
     7561 aagtagggga gcacgtcttt gtagccccag cccgtggcac cctgggccgc ccagccgtcg
     7621 tagtcactgg gggcaccgcg caggaacacg agaccattga tcgagcccga gccgcccagc
     7681 acgcgaccgc gcggccagct gagccggcgg ccgttgagct ggggttcggg ttccgtctcg
     7741 taaccccaat tgaccttggg attgggcacg agcttgccga agccgagggg gatatggatc
     7801 cagggattgc ggtcgcgctg tcccgcttcg atcaggcaca ggctgagttc gggatcctcg
     7861 gtgaggcgcg ttgccagcac gcagcccgcc gagcccgcgc ccgccaccac cacgtcgaac
     7921 tcggcatcga atgtatcgac caagacgttt cccctttccg cgatcgaccc ccacacgggg
     7981 tccccaatcc gtttgaaaat cttagcagag cgcggtgcgg tggcggggcc gagtgccccg
     8041 gcaccgtggc gctcctttgg ccgccgtcgc tgcctcaggg cgtggcgagg aacagcggga
     8101 tttgccggct cgtcttcgcc tgatagtcgg cgtagggcgg gaacgctgcg acggcgagct
     8161 tccacaagcg ctcgcgttcg gcggggtcct tcacttcgac gacgcgcatg ggccgcacga
     8221 cggtctcgtc cctgagctcc acctgggggt tggcgcggag attgtggacc caaacggggt
     8281 ccttcggcgc gccgccttgc gaggccacca ggacataacc gttgccgtcc ttgacgcgca
     8341 tcagcggcgt cttgcggatg gcgccggtct tgttgccgga gtgggtgacg aggaccaccg
     8401 gcaagccggt gtcgcgcagc gtggtgcctt tggtgccacc gctgctttcg taaagctcga
     8461 cctgctcacg aacccagtcg cgcgggcttg ggatatagtt ggccatgggc cctgccctcc
     8521 tgttgctgtc ggtgcccatt ttcgcacgag cccgccacag aagtgaaatg cgttttctga
     8581 aagggtcgca cgtcctgtct ggtgctcacc gagacttcgc cgcgccgccc gtccccgggg
     8641 tccgcgcgcg cgggctcagg acgcgcgcac caagcggaag ccgaggtggc ttgggggcgt
     8701 gccggccgag cagccgcccg cgcgcgcatc ccgaataaag tcgggcacgt aggcgcggtg
     8761 gcggccttcg accagccgga ccccgcagtt ggcgatctcg gaggcaacct cgccatcgcg
     8821 atcgagacgc gtgcgcacga agcagctgtc ggtccattcc cagacgttgc cggcgacatc
     8881 gagcagcccg tgctcgttgg ccccataggt ccccgtcggt tgcggcgcct tggcgagccc
     8941 ggcggcgcga tcccactctt gatcgtagcg ggccagcagg cgccggccgg ggtcggccag
     9001 ctccgcgctt tctgccagcg catcgtccgc gaggcggctc cccgcggcat aggcccactc
     9061 ccggtcggtc ggcaagcgaa acgccatgcc ggtcgtgcgc gacagccagg ccgcataggc
     9121 ggtggcgtcg cgccagctga ccttgacgac gggcgtgttg ggatccgccg catcctcgct
     9181 gccggcacag gctgcagcgc taacgcagcg ctgatattct gccgccgtca cctgctggcg
     9241 catgatcagc agcggacgct cgaggcgcgt caacaggcgc ggcggcgcgg cgggctggcc
     9301 atcgcgcagg acctcgccgg ctgcacggta gagaaagtcg gccggcggta aggcgacgag
     9361 gtcgggcatg ggaatggggg cctgcgccga ccacaggcgg ccggtgacgg cgcagaccgc
     9421 aagcaaaagc aacagtgcgg gcaagaggcg cttcaggctt tgagccgaca tcggccgccc
     9481 tcggtggcgc gagagcgctc agtccaggtt cgacttttgc acgggacctg gggctcggac
     9541 ctggctcatc aggtcctcgt tccagggacc ctcgatgcgg aagtgggcgg tggcgccaag
     9601 ctcgaaagcc tcgatcatat tgtgattgac gtaggcgacg acgccgggct gcaggaactt
     9661 atagagggca gcgccggcgg agccgccacg gatgaaccag gtttccaggt cggtctgcgg
     9721 cgggttggcg aatttccccg tttcccagac gtagtcgccg tgcccgccga cgaggtgcgg
     9781 acgcgagtcg cggtcggctt gggagtggat gatgaggaca gtctccccga ccttggccgg
     9841 catggcattc ttgccggtga gcgccccgac ggcgccgttg aagaccacgt gcgtcgggat
     9901 cagcttgtgc atgaggtcca tggtgtcggc gaaggcctcg acgtggctgg ggtaggactt
     9961 gaatttgccc ttttggtccc gcggtacgta gaagtcctgt tcgccgaggt aatagacgcg
    10021 gtcataggtg agcggcttgc cggcgccgtc cttgagacca tcgcgcggca gcaccatcac
    10081 ggcaccgctc atgccggcgg cgatgtgcca gggcaccatc ggcccgccgg ggatgcagtg
    10141 gtaaacgaag acgccggtgc gggtggcctt ccagcgcagc cgcacttgct cgcccggggc
    10201 gaccagggtc aggtcgccgc cgcccgaggc gccagtggcc gaatgaaagt cgatgttgtg
    10261 cggcatcgta ttgctggcga ggttgaccag ggtgacttcc acatagtcgc cctcgtgcac
    10321 gaccatcagc ggggcgggga tcgagccatt gaaggtcatg gccctgagct tggtgccctc
    10381 ctcgtcgatg acgatctgtt tttcttcggc gaccagccga aactccataa tcttcgggcc
    10441 ttcggaggtg gcctgctggt gggcgtgcac gtagggcggg gcgacgagct cgaccttttg
    10501 tcgcggcaaa ttgagatcgc ccgccgct
//
LOCUS       DASTMT010000329         1546 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_1822752, whole genome shotgun sequence.
ACCESSION   DASTMT010000329 DASTMT010000000
VERSION     DASTMT010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 1546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1546
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1822752"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            321..>1546
                     /locus_tag="VFR00_07685"
     CDS             321..>1546
                     /locus_tag="VFR00_07685"
                     /inference="COORDINATES: protein motif:HMM:NF040455.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEU0159178.1"
                     /translation="MGKVSLALLVVACCSLNQPALGQPAKAPLRPPLDLDAGAYPESG
                     PDPLTPPPVDAPPLLAPGATAPLPEAGQAREADPIVLAVRQRLASLPATRDGGEQADY
                     AALKTFYAQWTGPLWLGNGALTPRAEQAMLEINQADSWGLKAAAFDLPKLGAPSTDLE
                     TLADVEIKLSFAALKYARAARGGRLDPAAVSRKFDQKPPVYEPGSLLLAMAEQADVAA
                     YLRQLHPRHDQFQRLRQALLTARAAPPDPNTAATIRRLIVNLERWRWMPDELGDFYVW
                     DSVPEQMTRVVDRGEVTLSERIVVGKPSTPTPIFSANMQFVIFHPSWGVPAGMKVQEL
                     WPQLRNTGGGWFSSYPLASAVLAGHGLSVSRGGHPIKPDSVDWSKVDIREFEFVQPPG
                     AKNVLGIVKFRFPNKH"
BASE COUNT          262 a          549 c          484 g          251 t
ORIGIN      
        1 tctgttgaat tgcctctgtt gaactgccgg cccgtcgacg cgccgagccg caaggtttcc
       61 cgcgtaaagc gaggcgacgt gcttgccgca ccagtgccag gcgcgctcgg gccggcgccg
      121 gcgcgggcgc gacgggaatg agagtgagct tgagagtgag ttggggcgtg agcttgggcg
      181 atgcatttag atcacaagcg ctcaacatat gcgctcgcca gcgctcagat ccgcgaacag
      241 ggcgcggctc caacacacgg atggcactac cgtccgctac agcgcctgat tcttcggcaa
      301 acgattggcg gtgacgaaca atgggcaaag tcagcctcgc actccttgtc gttgcgtgct
      361 gcagcctgaa ccaacccgcg ctcggtcaac cggcaaaggc gccgctgcgc ccgccgctcg
      421 acctcgatgc cggcgcctac cccgaatccg gccccgaccc gctcacaccg cccccggtcg
      481 acgcaccgcc tctgttggca cctggggcga ccgcgccctt gcccgaggcg gggcaggcgc
      541 gcgaggccga tcccatcgtg ctggccgtgc ggcagcggct cgccagcctg cccgcgacgc
      601 gcgatggcgg cgagcaagcc gactacgcgg ccctcaaaac cttttatgcg caatggacag
      661 gcccgctttg gctcggcaat ggcgcgctga cgccgcgcgc cgagcaagcc atgctggaga
      721 tcaatcaagc cgacagctgg ggcctgaagg ccgccgcctt cgacctgccc aagctcggcg
      781 cgccgtcgac cgaccttgag acgctcgccg atgtcgaaat caaactgagc tttgccgccc
      841 tcaagtatgc ccgggcggcg cgcggcggcc gcctggatcc cgccgcggtt agccgcaagt
      901 tcgaccagaa gccccccgtc tatgagcccg gttcgttgct gctcgccatg gccgagcagg
      961 ccgacgtcgc agcctatcta cgccaactcc acccacgtca cgaccagttc cagcgcttgc
     1021 gccaggcgct gctcacggcg cgcgccgccc cgcccgaccc caacacggct gccaccattc
     1081 ggcgcctgat tgtcaatctg gagcgatggc gctggatgcc cgatgagctc ggcgactttt
     1141 acgtctggga cagcgtgccc gagcagatga cgagggtcgt cgatcgcggc gaggtgacgc
     1201 tgtcggaaag gatcgtggtc ggcaagccca gcacgcccac gccaattttc tccgccaaca
     1261 tgcaatttgt catctttcat ccctcctggg gcgtgcccgc cggcatgaag gtgcaggagc
     1321 tgtggccgca gctgcgcaac accggaggcg gttggttctc cagctatccg ctggcctcgg
     1381 cggtgctggc gggccacggt ctctccgtga gccgcggcgg acatcctatc aagcccgact
     1441 cggtcgactg gtcgaaggtc gacatccgcg aattcgagtt cgttcagccg cccggcgcca
     1501 aaaacgtcct gggcatcgtc aaattccgct ttcccaacaa gcacga
//
LOCUS       DASTMT010000330         3671 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_1823168, whole genome shotgun sequence.
ACCESSION   DASTMT010000330 DASTMT010000000
VERSION     DASTMT010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3671)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3671)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3671
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1823168"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            153..680
                     /locus_tag="VFR00_07690"
     CDS             153..680
                     /locus_tag="VFR00_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012563094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016531 - copper chaperone activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HEU0159179.1"
                     /translation="MLVFFPVRAQLRAALSRRAGLWLLAALACGIPYINAGAQSASYK
                     VGDLTIQAPWARATPAGAKVGGAYLKITNAGAQADRLVGGSLAGAREVEVHEMSMSNN
                     VMKMRHLKDGLEIKPGQTVELKPGSYHLMLTGLSEALKQGQKLKGSLTFEKAGSVEIE
                     YIVAPIGATSGGHAH"
     gene            complement(729..1556)
                     /locus_tag="VFR00_07695"
     CDS             complement(729..1556)
                     /locus_tag="VFR00_07695"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aspartate aminotransferase family
                     protein"
                     /protein_id="HEU0159180.1"
                     /translation="MYGPARAIAVNFLAKRGIACEFYAGDHREVEKRLKPETRLVYLD
                     NPGSIVYDIQDVPALARLLAGRSTLLAVDNTWGCPGLYRPLNLGADVSVVAVTKYLAG
                     HSDLVMGAVAAGPRCADQLWRDAALLGQTVSPDEAYTALRGLRTAAARLALHRAHAAE
                     VITWLKAQPEVKRVLYPALPEDPGHALWRRDFNGANGLLSVEFVAPITSAAADRLIDS
                     LSLFGIGASWGGFESLALTYPSIPGWHGGALVRLHIGLEDPADLIADLAQGFRAAFG"
     gene            complement(1480..1890)
                     /locus_tag="VFR00_07700"
     CDS             complement(1480..1890)
                     /locus_tag="VFR00_07700"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HEU0159181.1"
                     /translation="MGEREKTRLVRVSRGKAHGDVGTVNPPVVRCSTVLYRDIATRRD
                     VRARREQGERLFMYGASGTPTAFALEDAISEIEGGVRSVLLPTGLAAIAHVFLSLLRP
                     GDHVLLAETGVRASAGHCRQFSRQARDCLRILCR"
     gene            2128..2619
                     /locus_tag="VFR00_07705"
     CDS             2128..2619
                     /locus_tag="VFR00_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006115716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HEU0159182.1"
                     /translation="MPRKTKTAATRNDTPARPASGPAFWLFKSEPDSFSWEQQKARGK
                     KGEPWEGVRNFLARGNMKAMQLGDRGFFYHSNVGKEIVGIVEVCGLAHQDASDPTGVW
                     KCVDVRAIADMPKPVTLAAVKANRKLAQMVLVRSARLSVQPVTPAEWVEVCRMGGLDP
                     TTV"
     gene            3201..3401
                     /locus_tag="VFR00_07710"
     CDS             3201..3401
                     /locus_tag="VFR00_07710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0159183.1"
                     /translation="MQAFEQVEDFWSDTYRGRSIAILNRGGSWLVYLDHVMQHRMLFD
                     TAESAVRWLRRQIDQNRGARLH"
     gene            complement(3474..>3671)
                     /locus_tag="VFR00_07715"
     CDS             complement(3474..>3671)
                     /locus_tag="VFR00_07715"
                     /inference="COORDINATES: protein motif:HMM:NF020135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketopantoate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HEU0159184.1"
                     /translation="RPSMGQDMLKGRRTEIEFLNGYVVREATRLGLNCRANAALTEIV
                     KRVERGELVADPRHITDLRLN"
BASE COUNT          642 a         1215 c         1242 g          572 t
ORIGIN      
        1 tgccggggct caacgcggcc gcaactgcgc ccggcctcgg tcttcttctg cgtccacccg
       61 tgcgcgcacc gccactccgc agcgcctgat ttcagccccg gcggcaccgc ccgccgatct
      121 caccgcctga tcgccgtgga aggattttga gcatgcttgt gtttttcccc gttcgagccc
      181 aattgcgtgc cgcactgagc cgccgcgccg gcctttggct gcttgccgcc ttggcctgtg
      241 gcatccccta catcaacgcg ggcgcgcaaa gcgccagcta caaggtgggc gacttgacca
      301 ttcaagcgcc ctgggcacgc gctacgccgg ccggcgccaa ggtcggcggc gcctacctca
      361 agattaccaa cgccggggcg caggcggatc gcctcgtggg cggctcgctg gccggcgcgc
      421 gcgaggtgga ggtgcatgaa atgtccatga gcaacaacgt catgaagatg cgccacctca
      481 aggacggcct cgagatcaag cctggccaga cggtcgagct gaaacccggc agctaccatc
      541 tcatgctgac ggggctgagc gaggcactaa agcaggggca aaagctcaaa ggcagcctca
      601 ccttcgagaa agcgggatcg gtggaaatcg aatacatcgt cgccccgatc ggggcgactt
      661 cgggcgggca cgcccactga gccgcagcga gccccggcgc gcgccaacac ctacggggag
      721 ggcgcgcgct agccgaaggc ggcgcgaaag ccctgcgcca aatcggcgat gaggtcggcg
      781 ggatcttcca gcccgatgtg caagcgcacc agcgcgccgc cgtgccagcc ggggatggaa
      841 gggtaggtca gggccaagct ttcgaagccg ccccaggagg caccgatccc gaacaggctg
      901 aggctgtcga tcaggcggtc ggcggccgcg gacgtgatcg gggcgacaaa ttcgaccgac
      961 aagaggccgt tggcgccgtt gaagtcgcgc cgccacaagg cgtggcccgg atcttccggc
     1021 agggccgggt aaagcacgcg cttcacctcg ggttgcgcct tcagccaggt gatgacctcg
     1081 gcggcatgcg cccgatgcaa ggcgaggcgt gccgccgcgg tcctgagccc gcgcaaggcc
     1141 gtgtaggcct cgtccgggct caccgtctgt cccagcaaag cggcatcgcg ccagagctga
     1201 tcggcgcagc gcgggcccgc cgccaccgcc cccatgacga gatcggaatg cccagcgaga
     1261 tacttcgtga ccgcgaccac cgacacatcg gcgccgaggt tgagcggtcg ataaaggcct
     1321 gggcagcccc aggtattgtc cacggcgagc aaggtggagc gcccggcgag cagccgcgcc
     1381 agcgcgggga cgtcctggat gtcgtaaacg atcgatcccg gattatccaa gtagacgagc
     1441 cgggtttcgg gtttgaggcg tttttccacc tcgcggtggt caccggcata aaattcgcaa
     1501 gcaatcccgc gcttggcgag aaaattgacg gcaatggccc gcgctggccc gtacacccgt
     1561 ctcggctaac agcacatgat cgccgggccg caggagcgac aagaacacgt gggcgatggc
     1621 ggcaagaccg gtcggcaaca gcacgctgcg cacgccgcct tcgatctcgc ttatggcatc
     1681 ctcgagtgcg aacgcggtgg gtgtgccgct ggccccgtac ataaagaggc gttcgccctg
     1741 ttcgcgccgt gccctgacgt ctctgcgcgt ggcgatgtcg cggtaaagca ccgtcgagca
     1801 gcgcaccacg ggcgggttca ccgttccgac atcgccgtgc gctttgccgc gcgacacacg
     1861 caccagccgc gtcttttctc gctcgcccac gctcgccaac tccagctagg atagatgcgc
     1921 ctcgaagcca gccggcggcg aggcggcgag ccaaagcttg gggctcgcgc gcggtcgatg
     1981 caagcctcgc ctgtcactta cttgcggcgc aagctgcggc gcgcgggtag cgtgcgggtc
     2041 agtatgcccg ggtggcatcc ttcccgggtg cggtcgccaa gtgcgcgcgc caagcaggat
     2101 gtccgtagca gtttgggagt gctgtcaatg ccccgcaaga ccaagacggc agcaacgcgc
     2161 aatgacacac cggcgcggcc ggcatccgga cctgcctttt ggctcttcaa gtccgagccc
     2221 gacagctttt cctgggagca gcaaaaggcc aggggaaaaa agggcgagcc ttgggaaggc
     2281 gtgcgcaact tcctggcgcg cggtaacatg aaggcgatgc agctcggcga tcgcggcttt
     2341 ttttaccatt ccaacgtcgg caaagagatc gtgggcatcg tcgaggtttg cgggctcgcc
     2401 caccaggatg cgagcgaccc gacgggcgtg tggaagtgcg tcgacgtacg cgccatcgcc
     2461 gatatgccca agccggtgac gctggccgcg gtcaaggcca accgcaagct ggcccagatg
     2521 gtgctcgtcc gctcagcccg gctctcggtg cagccggtaa cgcccgcgga gtgggtcgaa
     2581 gtttgccgta tgggcggcct ggatccgacc acggtctgag acactggatg cggggcgcag
     2641 gggtctttgc tgcccacacc ggcggccccg tgcgtggccc cgtcctgtgg ccttcggccc
     2701 gcacgtctga agcggctgcc atcgggtccg cccaccgacg gttgccccca aacccggcag
     2761 cgtggggccc ggctgcccac gacttcagca ggtcagacct gatcggcagc gccggcggcc
     2821 gcggcgcgct gcttttttct ggcacgccct gccgttgccc gaggttcgcc ggggggtggc
     2881 caggacacgg cgccgcaccc ctgcgatttg cacgcacatt gcatggtcga ggcctgggcg
     2941 accgcagcgc ggggcccggg ttcgcctggc agcaagccct agcgccaggg ctctttggtc
     3001 agggcactta ggggccgcgt ggccgggagg gtctgggtcc aaggtctagg ggccaagggt
     3061 tctggcgagc gctgaggcgc caggtaaaag gaggaggata cgtggccgac ggagcgggaa
     3121 cgtcacgccg tttgcggggt taagcgggca aatggcccca cgcaaaccgg gccggaagcc
     3181 atgatcatga ggcaaacacc atgcaggcat tcgaacaggt agaggatttt tggtcggaca
     3241 cctaccgcgg acggagtatc gccattctca accgcggcgg cagctggctc gtttatttgg
     3301 accatgtcat gcagcaccgc atgctgttcg acacagcgga atcggcggtg cgctggctgc
     3361 gccggcaaat cgatcaaaac cgcggcgccc ggctgcattg agcgcagcgg tcccgtcttg
     3421 agagacacga cgtcggcggc aggccggcgt cgcagcaagt ctgccgatcc cgatcagttc
     3481 aagcgcaagt ccgtgatgtg gcgcgggtcg gccaccaact cgccgcgctc cacgcgcttg
     3541 acgatctcgg tcagggccgc attggcgcga cagttgaggc ccagccgcgt cgcctcgcgc
     3601 accacatagc cgttaagaaa ctcgatttcg gttcgcctcc ccttcagcat gtcctgaccc
     3661 atcgagggac g
//
LOCUS       DASTMT010000331         6850 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_6000934, whole genome shotgun sequence.
ACCESSION   DASTMT010000331 DASTMT010000000
VERSION     DASTMT010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 6850)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6850)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6850
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6000934"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..832)
                     /locus_tag="VFR00_07720"
     CDS             complement(<1..832)
                     /locus_tag="VFR00_07720"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018243285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit alpha"
                     /protein_id="HEU0159185.1"
                     /translation="MPMKLSRSAYADMFGPTTGDRVRLADTDLIVEVEKDLTSYGDEV
                     KFGGGKVIRDGMGQSQLSRAEGAVDTVITNALIIDHSGIYKADVGLRDGRIAAIGKAG
                     NPDTQAGVDIIIGPGTEAIAGEGKILTAGGCDSHIHFICPQQIEEALNSGITTMIGGG
                     TGPATGTAATTCTPGPWHLARMLEAADAFPMNLAFAGKGNASDPRALNEMIEGGASCL
                     KLHEDWGTTPAAIDCALAVADAHDVQVMIHTDTLNESGFVEDTIAAFKGRTIHAFHTE
                     G"
     gene            complement(832..1140)
                     /locus_tag="VFR00_07725"
     CDS             complement(832..1140)
                     /locus_tag="VFR00_07725"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003612706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0035550 - urease complex [Evidence IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit beta"
                     /protein_id="HEU0159186.1"
                     /translation="MVPGEIISAAGDIEINAGRQVITLRVANSGDRPIQVGSHYHFAE
                     TNGALSFDRARARGCRLDIPAGTAVRFEPGQTREVNLVPFAGRRRVFGFQGAVMGELD
                     "
     gene            complement(1183..1485)
                     /locus_tag="VFR00_07730"
     CDS             complement(1183..1485)
                     /locus_tag="VFR00_07730"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008121015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit gamma"
                     /protein_id="HEU0159187.1"
                     /translation="MNLTQREKDKLLISMAALVARRRLERGVKLNYPEAVALISDFVV
                     EGARDGRSVADLMEAGAHVVTRDQVMDGIAEMISDVQVEATFPDGVKLVTVHQPIR"
     gene            complement(1503..2324)
                     /locus_tag="VFR00_07735"
     CDS             complement(1503..2324)
                     /locus_tag="VFR00_07735"
                     /inference="COORDINATES: protein motif:HMM:NF013899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreD"
                     /protein_id="HEU0159188.1"
                     /translation="MLQAVDWLPRTEGSARLGLVRRGERTRLARLEQSGAARLKFPRQ
                     GQGAPCEAVLVNTAGGLTGGDSFHIAAALGPGTEATLTTAAAEKIYRARDGTAAVSVS
                     LCVAAGASLAWLPQPTILYDRAALERTTRVELGAEASFLGLEILIFGRAAMGESVRWG
                     SLHDQWRVWREGRLLFADAVRLEGPIAERLARPALLGGAGAMGLVLYVAASAQERIES
                     ARAILHTAGAAAGVSAFNGLLLARAVAADGRVLQAQLTPLLQALHGRPLPRIWSC"
     gene            complement(2352..3047)
                     /gene="urtE"
                     /locus_tag="VFR00_07740"
     CDS             complement(2352..3047)
                     /gene="urtE"
                     /locus_tag="VFR00_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018260852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter ATP-binding subunit UrtE"
                     /protein_id="HEU0159189.1"
                     /translation="MLSVENIDLHYGAALALRDVSLTAAPGKITCVLGRNGVGKSSLL
                     RAIIGLQPVSRGRVLFNGSEVTRLRTHERARLGMAYVPQGREIFPLLTVRENLETGFA
                     RLPRRERKLGAELFNLFPVLKSMLGRRGGDLSGGQQQQLAIARALIMQPTLIVLDEPT
                     EGIQPSIIKDIGRAIAFLRQEGKMAIILVEQYFEFARELADTYAVMERGEVVLAGEGK
                     TMVEAEVRRYLTV"
     gene            complement(3128..3898)
                     /gene="urtD"
                     /locus_tag="VFR00_07745"
     CDS             complement(3128..3898)
                     /gene="urtD"
                     /locus_tag="VFR00_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019404072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter ATP-binding protein UrtD"
                     /protein_id="HEU0159190.1"
                     /translation="MDLSVAKPHEITPSLLYLDGVSVSFDGFRALNNLSLDISPGEMR
                     AIIGPNGAGKTTMMDVVTGKTRPDSGAVLFDKGIDLTSKDEAAIAQLGIGRKFQKPTV
                     FEHHSVEDNILLALKADRGVRANLAWSRNPHDAERIGEILATIGLGGQRQRLAGALSH
                     GQKQWLEIGMLIAQDPKLLLVDEPVAGMTDAETMTTAELLRDIAKDHTVIVVEHDMEF
                     VRRLGVKVTVLHEGSVLAEGSIDEVSANARVVEVYLGR"
     gene            complement(3905..5140)
                     /gene="urtC"
                     /locus_tag="VFR00_07750"
     CDS             complement(3905..5140)
                     /gene="urtC"
                     /locus_tag="VFR00_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008195045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter permease subunit UrtC"
                     /protein_id="HEU0159191.1"
                     /translation="MITRRLMEAPDGLGRIFLALLLGLAVLVPVLNLALPPASPLHVP
                     ISTVALWGKYLCYALLALSLDLVWGYCGILSLGHGAFFALGGYAMGMYLMRQIGTRGV
                     YAHPVLPDFMVFLNWPELPFYWWGFNWFGYALLMVFLVPGALAFVFGWFAFRSRVTGV
                     YLSIITQALTYALMLGFFRNNFGFGGNNGLTDFKDILGFNVQAAPTRAALFALSALAL
                     AACYLLAQAVVRSKAGKILVAVRDEESRTRFLGYRVENYKTFVFTLSAGMAALAGALY
                     VPQVGIINPSEFAPANSIEVVIWVAVGGRGTLVGAALGAGLVNYAKTTFTTGLLAPYW
                     LFALGGLFVLVTIFLPRGLLGLIRKGPPPAAPAADTPSRRLDDSRRDVTRTAAPAGEL
                     QRQARAHVGPATGIVQPAE"
     gene            complement(5137..6744)
                     /gene="urtB"
                     /locus_tag="VFR00_07755"
     CDS             complement(5137..6744)
                     /gene="urtB"
                     /locus_tag="VFR00_07755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008964721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter permease subunit UrtB"
                     /protein_id="HEU0159192.1"
                     /translation="MRVSERWAAVVLSLLVLLAGSLGALAADSWEAALSKFAGDSYND
                     TDAAIAAVAASGNPLAADVIEALQDGRLSFGEDKRVYLRDKSGKLSDAATGTPADVAA
                     AALKPVRINNRIRRSIEAALGGLTLLSTDPGRRLEAAQAVFKSKDAKALAALEQAIAR
                     EADPTIKRALEEARAAVLLYSATASEGEKLEAVGQIRRRGDQDARGLLAALPAEQPET
                     VRRVVADAIADIDNRLAAWGVVQNLWYGLSLGSVLLLAAIGLAITFGVMGVINMAHGE
                     MVMLGAYTTFLVQEVIRGSAPWLFDYSLAIAIPLAFLVAGLIGIAIERGVIRFLYGRP
                     LETLLATWGISLILQQAVRTSFGPTNREVGAPHFMAGAFELGGLTITWNRLWIIVFAG
                     IIFALLLAALRFTTLGLHMRAVTQNRRMSSAMAIPTGRIDALTFALGSGIAGLAGVAL
                     SQIDNVSPNLGQGYIIDSFLVVVFGGVGNLWGTLVGALSLGVANKFLEPYAGAVLGKI
                     LLLVIVILFIQKRPRGLFALKGRAVEA"
BASE COUNT         1197 a         2325 c         2253 g         1075 t
ORIGIN      
        1 ccccttcggt gtgaaaggca tggatggtgc gacccttgaa ggccgcgatg gtgtcttcca
       61 cgaagccgga ttcgttcagc gtgtccgtgt ggatcatcac ctgcacgtcg tgagcatcgg
      121 ccaccgccag ggcacagtcg atggcggccg gcgtcgttcc ccaatcctcg tgcagtttga
      181 ggcaggaggc gccgccctca atcatttcgt tgagcgcgcg cggatccgaa gcgttgccct
      241 tgccggcgaa agccagattc atcgggaagg cgtcggcggc ctcgagcatg cgggccaggt
      301 gccaagggcc gggcgtacag gtggttgccg ccgtgccggt ggcgggaccc gtgccacccc
      361 cgatcatggt ggtgatgccg gaattgagcg cctcctcgat ctgctgcgga cagatgaagt
      421 ggatgtggct gtcgcagcct cccgcggtca ggattttgcc ttcccccgcg atcgcctcgg
      481 tgccggggcc gatgatgatg tcgacgcccg cctgggtatc gggattgccg gccttaccga
      541 tggcggcgat gcggccgtca cggagaccga catcggcctt gtagatgccg ctgtggtcga
      601 tgatgagggc gttggtgatg acggtgtcca ccgcgccctc ggcgcggctg agctggcttt
      661 gtcccatgcc gtcgcggatc accttgccgc caccgaactt gacctcatcg ccgtagctcg
      721 tgagatcttt ttcgacctcg acgatcaggt cggtatcggc aagacgcacg cgatcgccgg
      781 tggtcggacc gaacatgtcg gcataggccg agcgcgacag tttcattggc atcagtcgag
      841 ctctcccatg acggcgccct gaaaaccgaa cacccggcgg cggccggcga aaggcaccag
      901 gttgacctcg cgggtctgcc cgggctcgaa gcgcaccgcc gtcccggccg gaatgtccag
      961 gcggcagccg cgcgcgcggg cacggtcgaa gctcagcgcg ccattggttt cggcgaaatg
     1021 atagtgcgac cccacctgga tcgggcggtc gccgctattg gcgaccctca gcgtgatgac
     1081 ctggcggccg gcattgatct cgatgtcgcc ggccgcgctg atgatttccc cgggaaccac
     1141 gacaaacctc ctctattgag cggcaaaggc agaaacccgt gcctatctga tcggctggtg
     1201 caccgtcacg agcttgaccc cgtcgggaaa ggttgcctcc acctgcacgt cactgatcat
     1261 ttcggcgatg ccgtccatga cctgatcgcg cgtcacgaca tgggcacccg cttccatcag
     1321 atcggcaacg ctgcggccat cgcgcgcgcc ctccaccacg aaatcggaaa tcagcgccac
     1381 ggcctcggga tagttgagct tgacgccgcg ctcgagacgc cgtcgcgcga cgagcgcggc
     1441 catggaaatc aggagcttgt ccttttcgcg ctgggttagg ttcatctctc accttagggc
     1501 tttcagcaac tccagatgcg cggcagcggc cggccgtgca aggcttgcag cagcggcgtc
     1561 aactgggcct gcagcacgcg cccatccgcg gcgacggcgc gcgccagcaa caggccgttg
     1621 aaggcgctga cgccggccgc ggcaccggca gtgtgcagga tggcgcgggc gctttcgatg
     1681 cgctcctggg cgctagccgc cacgtaaagc accaggccca tcgcgccggc gccgccgagc
     1741 agcgccgggc gggcgaggcg ctcggcgatc ggaccctcga ggcgcacggc atcggcaaac
     1801 agcaaccgcc cctcgcgcca cacccgccat tgatcgtgca agctccccca ccgcacgctt
     1861 tcccccatgg ccgcccggcc aaagatcaag atctccaagc ccaggaagct tgcctccgcg
     1921 cccagctcga cccgggtcgt gcgctcgagc gcggcgcggt catagaggat ggtgggctgc
     1981 ggcagccacg ccaggctggc gccggccgcg acgcacaaac tgaccgagac ggccgccgtc
     2041 ccgtcccgcg cgcggtagat cttttcggcc gccgccgtcg tcaaggtggc ctcggtgccc
     2101 gggccgagcg cggccgcaat gtggaagcta tcgcccccgg tgaggccgcc ggccgtattc
     2161 accagcacgg cctcgcatgg cgccccctgg ccttgccttg gaaatttgag gcgcgcggcg
     2221 cccgactgct cgagccgtgc gagccgcgtg cgttcccccc ggcgcaccag gcccagtcgc
     2281 gccgatcctt cggtgcgcgg cagccagtcc acggcctgga gcacgggcgg atccttttgg
     2341 aaggttgcgg ttcagacggt aaggtagcgc cgaacctctg cctcaaccat ggtttttccc
     2401 tcgcccgcca gcaccacttc gccgcgctcc atcaccgcat aggtgtcggc gagctcgcgg
     2461 gcgaactcga aatattgctc gaccaggatg atcgccatct tgccctcctg gcgcagaaag
     2521 gcgatggccc ggccgatgtc cttgatgatg gagggttgga tgccctcggt cggctcgtcg
     2581 agcacgatca gcgtgggttg catgatcagc gcgcgggcga tcgccaactg ctgttgctgg
     2641 ccgcccgaca ggtcgccgcc ccggcgcccc aacatactct tgagcaccgg gaacaaattg
     2701 aagagctcgg cgccgagctt gcgctcgcgc cgcggcaggc gcgcaaagcc ggtttccaaa
     2761 ttctcccgta ccgtcagcag ggggaagatc tcgcggccct gcggcacata agccatgccg
     2821 agccgcgcgc gctcgtgcgt cctgagccgg gtgacctcgc tgccgttgaa gaggacgcgg
     2881 ccgcggctga ccggctgcaa gccgatgatc gcccgcagca gcgagctctt gcccacgcca
     2941 ttgcggccca gcacgcaggt gattttgccg ggcgccgccg tcagcgagac gtcgcgcagg
     3001 gcaagtgctg ccccatagtg caaatcgatg ttctcaacgc tcagcattgc cttgaacctc
     3061 acgtgcgcgc cgcggcgccc tggccgggcc ggtgcggccg ccgcaccact tatgccgcgc
     3121 ccagccccta gcgccccaag tacacctcca ccacccgcgc attggcgctc acctcgtcga
     3181 tactgccttc ggccagcacc gagccctcgt gcaggacggt caccttgacg ccgagccggc
     3241 gcacgaactc catgtcgtgc tcgacgacga tgacggtgtg gtccttggcg atatccctga
     3301 gcagctcggc cgtggtcatg gtctccgcgt cggtcatgcc ggcaaccggc tcgtcgacga
     3361 gcaggagctt gggatcctgg gcgatcagca tgccgatttc gagccattgc ttttgcccgt
     3421 gcgagagcgc gccggccagg cgctggcgct gcccgccgag accgatggtg gcaaggattt
     3481 cgccgatgcg ttccgcatcg tgcggattgc gcgaccaggc caggttcgcg cgcacgccgc
     3541 gatcggcctt cagcgccagc aaaatattgt cctccaccga gtgatgctcg aacaccgtgg
     3601 gtttctggaa cttgcgcccg atgccgagct gggcgatggc ggcctcgtcc ttgctggtga
     3661 gatcgatacc cttgtcgaac agcaccgcgc cgctgtcggg ccgggtcttg cccgtcacca
     3721 catccatcat cgtggttttg cccgccccgt tcgggccgat gatggcgcgc atttcgccgg
     3781 gcgaaatatc gagcgacaga ttgttgagcg cccgaaagcc atcaaaactg acgctcaccc
     3841 cgtcgagata gagcaggctc ggcgtaatct cgtgcggctt ggcaacggaa agatccatgg
     3901 ctcttcactc cgccggctgc acgatgccgg tcgctgggcc aacgtgcgcg cgggcctggc
     3961 gctgcaattc cccggccggg gcggccgtgc gcgtgacgtc cctgcggctg tcatcgagcc
     4021 gccgcgaggg cgtgtcggca gccggggccg ccggcggcgg gcctttgcgg atcaggccga
     4081 ggagcccccg gggcaggaag atcgtcacca gcacgaacag gccgcccagg gcgaacagcc
     4141 aataaggcgc gagcaacccc gtggtgaacg tggtcttagc atagttcacc agcccggcgc
     4201 cgagggcggc accgaccagc gtgccgcgtc ctccgaccgc cacccaaatg acgacctcga
     4261 tcgagttggc cggggcgaac tcgctggggt tgatgatgcc gacctgcggc acatagagcg
     4321 cgccggccag cgccgccatg cccgccgaca gggtgaacac gaaggttttg tagttttcca
     4381 cgcggtagcc gagaaagcgc gtgcggcttt cctcatcgcg caccgccacc aggattttgc
     4441 cggccttgga acgcaccacc gcctgggcca ggaggtagca ggccgccagg gccagggcgg
     4501 agagggcgaa taaggccgcc cgcgtcggcg ccgcctgcac gttgaagccc agaatgtcct
     4561 tgaagtcggt gagaccgttg ttgccgccaa aaccgaaatt gttgcggaaa aaccccaaca
     4621 tcaaggcata ggtcaaggct tgcgtgatga tcgagaggta gaccccggtc acccggctgc
     4681 gaaaggcaaa ccagccgaac acgaaggcta gcgcgcccgg caccaggaac accatcaaga
     4741 gcgcatagcc gaaccaattg aagccccacc agtagaaggg cagctcgggc cagttgagaa
     4801 acaccatgaa gtccggcagc acgggatgcg catagacgcc gcgggtgccg atctggcgca
     4861 tcaagtacat gcccatggca tagccgccca gcgcgaagaa ggccccgtgc ccgagcgaaa
     4921 ggatgccgca gtagccccag acgagatcga gcgagagcgc gagcagcgca tagcacagat
     4981 acttgcccca cagcgccacc gtggagatgg gcacgtgcag gggcgaggcg ggtggtagcg
     5041 ccaggttgag gaccggcacc agcacggcaa gccccaacag gagggctaag aagatgcggc
     5101 cgaggccgtc gggagcctcc atgagccggc gggtgatcat gcttctaccg ctcggccttt
     5161 cagcgcgaac aggccgcgcg gacgtttctg gatgaacagg atcacgatca cgagcaacag
     5221 gatcttgccg agtacggcgc cggcgtaggg ctccaggaac ttgttggcga cgcccagcga
     5281 cagggcgccg accagcgtac cccagagatt gccgacgccg ccgaacacca ccaccaagaa
     5341 gctgtcgatg atgtagccct gacccaggtt cggcgagacg ttgtcaattt gggaaagcgc
     5401 cacgccggcg aggccggcga tgccggagcc cagtgcaaag gtcaaagcat caatgcggcc
     5461 ggtgggaatc gccatggcgc tggacatgcg ccggttctgg gtcaccgccc gcatgtgcag
     5521 cccgagcgtc gtgaagcgca gcgccgccag cagcaaggca aagatgatgc cggcaaagac
     5581 gatgatccac agcctgttcc aggtgatcgt caggccaccg agctcgaatg cccccgccat
     5641 aaagtgcggc gcacccactt cgcgattggt gggcccgaaa ctggtgcgca cggcctgctg
     5701 caggatcaag ctgatgcccc aggtcgccag cagcgtctcg agcgggcgac cgtagaggaa
     5761 gcggatcacc ccacgctcga tggcgatgcc gatcagcccc gccaccagga aggcgagcgg
     5821 gatggcaatc gccagcgaat agtcgaacag ccagggcgcc gagccccgga tcacctcctg
     5881 caccaggaag gtggtgtagg cgcccagcat caccatttcc ccgtgcgcca tgttgatgac
     5941 gcccatgacg ccgaaggtga tggcaaggcc gatcgcggcc agcagcagca cggaaccaag
     6001 cgacaggccg taccaaaggt tctgcaccac gccccaggcg gcgaggcgat tgtcgatgtc
     6061 ggcgatggcg tcggcaacga cacgccgcac ggtctcgggc tgctcggcgg ggagcgcggc
     6121 gagcaggccg cgcgcgtctt gatcgccgcg ccgccggatc tgtccgaccg cctcaagctt
     6181 ttccccctcc gaggcggtgg cggaataaag cagcacggcc gcccgtgcct cctcgagcgc
     6241 gcgcttgatg gtggggtcgg cctccctggc gatcgcctgc tccagcgcgg cgagcgcctt
     6301 ggcatccttg gacttgaaaa cggcctgggc cgcctccagg cgccgtcccg gatcggtcga
     6361 gagcagggtc agcccaccca aggccgcctc gatgctgcgc cggatgcggt tgttgatgcg
     6421 cacgggcttg agtgcggccg cagcgacgtc ggccggcgtg ccggtcgccg cgtcgctgag
     6481 cttgccgctt ttgtcgcgca gatagacgcg cttgtcctcg ccgaaggaaa ggcgaccgtc
     6541 ctgcagcgcc tcgatcacgt ccgccgccag ggggttgccg ctggccgcga cagcggcgat
     6601 cgccgcgtcg gtgtcgttat aggaatcgcc ggcaaacttt gaaagcgccg cctcccagct
     6661 gtcggcggcc agggcgccaa ggctccctgc cagcagcaca agaagcgaca atacgaccgc
     6721 cgcccagcgt tcgcttaccc gcatgctcca agcttcccgg tatcagatga gggggcggcg
     6781 ccgatcgccg cccccaaacg tctcagcgct gcagtcgcgc taactgccgc acttgttggt
     6841 cttggtgttg
//
LOCUS       DASTMT010000332         2583 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_2155661, whole genome shotgun sequence.
ACCESSION   DASTMT010000332 DASTMT010000000
VERSION     DASTMT010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2583)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2583)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2583
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2155661"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(127..2298)
                     /locus_tag="VFR00_07760"
     CDS             complement(127..2298)
                     /locus_tag="VFR00_07760"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HEU0159193.1"
                     /translation="MPDIHALLWPRHVALIGASAELESLRGRIMHTMLSHPFEGQIYP
                     VTRSHAEVLGRKAYASVRDLPQRPDLAVLIIPAAYIPRALEDCGRAGAKAAVVLSSGF
                     AEAVGADGSRLQAEIRAIAKRYDLAVVGPNAEGFANLPAALVPTFSPALEASERPLLP
                     SGPRRGQITVLAQSGGIGFSFFDHGRARELAFRYIVTTGNEACLETMDIAEYALEEGK
                     TDALIVLLEDVKSPQTLRRVAEKALRAGVPIITAKIGRSEAGVRAAASHTAALAGSYQ
                     AYRAMFERYGLIEGHDIDEMIDLAHAMIAWRGRLPAGKRVGICTASGGGGGWMADTCA
                     AAGLEVPMLDAATRARVDQHLPAYGTSQNPVDATAQAVHKIGYAGLARLVLDAPALDG
                     LVIVITARSSKNLARQEAALAQLAAETTKPILLWSYTLPAPQSSKLLADAGYPLFTDI
                     RNCARAFAVMADYRALREAFLKPIEVSARETVPDRAAARAQLKASDSVLCHWEVRPLL
                     AAYGIGTLPEGQLVHSAAAAVEAAASLGGQVALKVQSPDIAHKTEAGAVRLNLFGAEA
                     VGAAYGDLIAAAQRHAPSARIHGVLVEAMVLPGQEMILGIKRDHTFGPLLLVGLGGVN
                     VELVQDVVLAPVPLSHASARDLLRRLKGARLLNGYRGRPASDVDALVEAMVRLGRLAQ
                     DFNDEIDEIDLNPIIVHPEGQGISVVDALLVKARPAAGPPA"
BASE COUNT          442 a          824 c          908 g          409 t
ORIGIN      
        1 agcgcgagct tcaaggccat tgcgccagtc ctcccagagt tgcccaccga tcagtgacca
       61 ggggcttatc gttgtcttcg agtacgctag ccgccacgtc gccggcgagc aagccccgcc
      121 tttggcctac gccggcggcc ccgctgcggg acgcgctttg acgagcagcg catcgaccac
      181 ggagatgccc tgaccttcgg ggtgcacgat tatgggatta aggtcgattt cgtcgatctc
      241 gtcgttgaag tcctgtgcca gccggcccaa acgcaccatg gcttcgacga gggcatcgac
      301 atcgcttgcc ggccgacccc gatagccgtt gaggagccgc gcgcccttga gacgccgtag
      361 gaggtcgcgg gcgctggcat gggatagcgg cacgggggca aggacaacat cctgcaccag
      421 ctcgacgttc acgccgccaa ggccgaccag cagcaggggg ccgaaggtgt gatcgcgttt
      481 gatacccagg atcatttcct ggccgggcag gaccatggcc tcgaccagga cgccgtggat
      541 gcgcgccgac ggcgcgtgcc gctgggcggc ggcgatgagg tcgccatacg cggcgccgac
      601 cgcctcggcg ccgaaaaggt tgagcctcac cgcacccgcc tcggtcttgt gcgcgatatc
      661 gggagattgc accttgagcg cgacctgccc gccgaggctg gcggcggctt cgaccgctgc
      721 cgcggcggag tggacaagtt ggccctccgg cagcgtaccg atgccgtagg ccgccagcag
      781 cggccgcacc tcccagtggc agagcacact gtcgctggcc ttgagctggg cacgcgccgc
      841 ggcgcgatcg ggtaccgtct cccttgctga gacctcgatg ggcttcagaa aggcctcgcg
      901 cagggcgcgg tagtccgcca tgacggcaaa ggcgcgcgcg caattcctga tatcggtgaa
      961 gagcggatag ccggcatcgg cgaggagctt gctcgattgc ggcgccggca gcgtatagct
     1021 ccacagcagg atgggtttcg tcgtctcggc tgccagctgc gccaacgcgg cctcttgccg
     1081 cgcaaggttt ttcgacgagc gcgcggtgat gacgatcacg aggccatcga gggcgggcgc
     1141 gtcaagcacg aggcgcgcca ggccggcata gcctatcttg tgcacggctt gtgcggtcgc
     1201 atcgaccggg ttttgtgagg tgccataggc gggcaggtgt tgatcgaccc gggcgcgcgt
     1261 tgccgcgtcg agcatgggca cttcgagccc agccgcggcg caggtatccg ccatccagcc
     1321 accgccaccc cccgatgccg tgcagatgcc gacccgtttg cccgccggca accgcccgcg
     1381 ccaggcaatc atcgcatggg caaggtcaat catctcatcg atgtcgtggc cctcgatcag
     1441 accataacgc tcgaacatgg cgcggtaggc ctgatatgaa ccggccagcg ccgcggtatg
     1501 tgaggcggcg gcccgcacgc cggcttcgga tcgaccgatc ttggccgtga tgatgggaac
     1561 tccggcgcgc aacgcctttt cggcgacccg ccgcaaggtc tgagggctct tcacatcctc
     1621 caagagcacg atcagtgcgt cggtcttgcc ctcctcgagt gcgtactcgg caatgtccat
     1681 cgtctccaga caggcttcat tgccggtggt gacgatgtag cggaacgcca gctctctggc
     1741 gcgaccgtga tcgaagaagg aaaagccgat gccgccgctt tgcgccaaca ccgtgatctg
     1801 tccgcgccga ggcccgctcg gcagcaacgg gcgctcggag gcctcaagcg ccgggctgaa
     1861 ggtggggacg agggcggcgg gaagattggc gaagccctcc gcgttggggc cgaccacggc
     1921 caagtcatag cgcttggcaa tggcacgaat ctcggcctgc aggcgcgacc cgtctgcgcc
     1981 aacggcctcc gcaaaaccgg aactcagcac gacggcggcc ttggcgccgg cgcgtccgca
     2041 gtcctccagg gcccgcggaa tataggctgc cggaatgatg aggacggcga ggtcggggcg
     2101 ctgcggcagg tcgcggacgg aagcataggc cttgcgcccg agcacctcgg catggctgcg
     2161 ggtcacggga tagatttggc cctcgaaggg gtgagagagc atggtgtgca taatgcgacc
     2221 gcgcaggctt tcgagctctg cggaggcacc gatcagcgcg acgtggcgcg gccagagcag
     2281 ggcatgaatg tcgggcatag caacctggtg ggcggggagg cggcagcgtg gctggcagta
     2341 gagcgcagcg tctcgggctt gtcgatgggt cgccgcggtt ggccgccgca gcatcgactt
     2401 gtataaggcc tgggcgcgaa cgttttacga ggcgtggagg cggcgtgcag aacgagatcg
     2461 caggtggcgg cgacgacggc gcgggccacg caaggtcaac ggcggggcac ctctaccatg
     2521 aggatttaga ggtcggtcgg ccctatctgt ccggcagcaa gacggtcaca gccgaggaga
     2581 tcg
//
LOCUS       DASTMT010000333         3487 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_3139635, whole genome shotgun sequence.
ACCESSION   DASTMT010000333 DASTMT010000000
VERSION     DASTMT010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3487)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3487)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3487
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3139635"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..488
                     /locus_tag="VFR00_07765"
     CDS             <1..488
                     /locus_tag="VFR00_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007599583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU0159194.1"
                     /translation="QAITGSGWPSYFIEDYAAQAAGFHTQLGSLICEGVFVKYPKLKV
                     VLIESGVSWLPPYLWRLAKSWRGVRQEVPWLDRSPEEFARDHVRLTLQPFDAPPDAAH
                     IGRIMDQLQSDTMLLFATDFPHWQFDDEAMLPAGLPAELKRKILIDNAFSTYPRLGRS
                     P"
     gene            485..1618
                     /locus_tag="VFR00_07770"
     CDS             485..1618
                     /locus_tag="VFR00_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008970061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU0159195.1"
                     /translation="MNLDAPFLAEPRQESAADPERQPKARLQIIDGDVHPALRTPADL
                     KPYLSQRWWEHFQTWGGRRRSGMSYEPYPKSQPRASRRDAWPAEGGAPGSSLRLMREQ
                     YLDAYGIEFGILGPLGLTGQSEFNLDFAGAVASAVNDWQRDTFSVPEPRLKSAIVVPY
                     DDGEAAAREIDKRADDPAFAQVFMLTRSQEPAGRKRYWPIYAAAERHSLPVGMHVFGA
                     SGHPYTGAGWPSYYIEESSGHSTSCQTVVTSLVMEGVFERFPKLKVVIIEGGFGWLPA
                     LTWRLDKLFERMRSEVPHLKRRPSEYIREHIWLTTQPMEEPKDPRQLLDVMEWIGWDR
                     LLFASDYPHWDFDDPLRAFPAALSRERLQQILTGNGKAVYRLA"
     gene            1618..2034
                     /locus_tag="VFR00_07775"
     CDS             1618..2034
                     /locus_tag="VFR00_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018321371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HEU0159196.1"
                     /translation="MPRHVVARAEDLPPGTRKLVHVAGRDIVILNVKGELFALSDKCP
                     HKGGSLARGKLTGLVLSAEPGTYHYSRAGEMLRCPWHGWEFDVRTGRSWCDPDRLRLM
                     NYRVSVEPGAAVVAGPYVAETFAVTVEDAYVVVETK"
     gene            2272..3243
                     /locus_tag="VFR00_07780"
     CDS             2272..3243
                     /locus_tag="VFR00_07780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011088786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEU0159197.1"
                     /translation="MALIARLGIVVAAALALAQPAAAQDFPNHPVKIIVPFGAGGPAD
                     VFARIIGHHLQEALKQSFIIENRPGAGAVIGTNEVGKSAPDGYTLLMMSNTHTVNETL
                     LPNRNYQLLRDFTAVAPVNSSDLLLVVHPSVPAKDLKEFLALLKAKPGELNYASSGPG
                     TPYHMAAELFKSMSGTNLVHVPHRGSGDARTSVIGGHVQMMIDAITTMAPTARDGQVR
                     ALGSTGQTRSSVMPDVPTIAEAGLPGYEATIWLGIMAPAGTPKPIVDKLNAEINKVLS
                     LPDLKAQWAQQGAQPLIMMPAEFESYLRKDIEKWAKVIETAGIKVQQ"
     gene            3240..>3487
                     /locus_tag="VFR00_07785"
     CDS             3240..>3487
                     /locus_tag="VFR00_07785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006023577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEU0159198.1"
                     /translation="MTAMAPEPTTLMVNGVERTVSADAATPLLNVLRAELGLTGTRFG
                     CGANACGACNVIIDGEAVASCDTPLWAAAGKRITTIEGL"
BASE COUNT          601 a         1215 c         1080 g          591 t
ORIGIN      
        1 accaggcaat tacggggtcc ggctggccca gctactttat cgaggattac gcagcgcaag
       61 cggcgggctt ccacacgcag ctggggagcc tgatctgcga gggcgtgttc gtcaaatatc
      121 ccaagctcaa ggtggttttg atcgaatcgg gcgtgtcctg gcttcccccc tatctctggc
      181 gcctggccaa aagctggcgc ggggtgcgcc aagaggtccc ttggctcgac cgctcgccgg
      241 aagagtttgc ccgcgaccac gtgcggctca cgctgcaacc ctttgacgcg ccgcccgacg
      301 cggcgcacat tggcaggatc atggatcagc tgcagtcgga taccatgctg ctgtttgcca
      361 ccgactttcc gcactggcag ttcgacgacg aggccatgct gccggccggt ctgccggccg
      421 agctcaagcg caagatcctg atcgacaacg cattttccac ctatccccgt ctcgggagat
      481 ccccatgaac ctcgacgccc ccttcctcgc ggagcccagg caggagagcg cggccgatcc
      541 cgaacgccag cccaaggccc gcctgcagat catcgacggc gacgtgcacc cggccttgcg
      601 cacgccggcc gacttgaagc cctacctgtc gcagcgctgg tgggagcatt tccagacctg
      661 gggcggtcgg cggcgcagtg gcatgagcta tgagccctat ccaaaatcgc agccgcgggc
      721 gagccggcgc gacgcctggc cggccgaggg cggtgccccg ggcagcagct tgcgcttgat
      781 gcgcgagcag tacctcgacg cctacggcat cgaattcggt atcctgggtc ccctcgggct
      841 gaccgggcag agcgagttca acctcgactt tgccggcgcg gtcgcctccg ccgtcaacga
      901 ttggcagcgc gacaccttca gcgtgcccga gccgcgcctc aagtccgcaa tcgtcgtgcc
      961 ctacgacgat ggggaggcgg ccgcgcggga gatcgacaag cgcgccgacg atccggcctt
     1021 tgctcaagtc tttatgctga cgcgcagcca ggaacccgcc ggccgcaagc gctattggcc
     1081 gatctacgcc gccgccgagc ggcactcact tcccgtcgga atgcacgtgt tcggcgccag
     1141 cggccatccc tacaccgggg ccggctggcc gtcctactac atagaagaaa gctccggcca
     1201 ctccacctcc tgccagaccg tcgtcacctc attggtgatg gagggcgtct tcgagcgctt
     1261 tcccaagctc aaggtggtga tcatcgaagg cggcttcggc tggctgccgg ccttgacctg
     1321 gcggctcgac aagctgttcg agcgtatgcg cagcgaggtg ccgcatctca agcggcgacc
     1381 gtcggaatat atccgcgagc acatctggct caccacccag ccgatggagg aaccaaagga
     1441 tcctcgccag ctcttggacg tgatggaatg gatcggctgg gaccggctgt tgtttgccag
     1501 cgactatccg cactgggact tcgacgatcc cttgcgcgcc tttcccgcgg cgctttcccg
     1561 cgagcgcttg caacagatcc tgaccggtaa cggcaaggcg gtctatcggc tcgcctgatg
     1621 ccgcgccacg tcgtcgccag ggctgaggac ctgcctcccg gaacgcgcaa gctcgtgcat
     1681 gtcgcggggc gcgacatcgt catcctcaac gtcaagggtg agctgtttgc actctccgac
     1741 aagtgtccgc acaagggcgg cagcctcgcc cgcggcaaac tcaccgggct cgtgctttcg
     1801 gcagaaccgg gcacctatca ttactcgcgc gccggcgaga tgctgcgctg cccctggcat
     1861 ggctgggagt tcgacgtgcg caccgggcgc tcctggtgcg atcccgaccg gctgcggctc
     1921 atgaactatc gcgtcagcgt cgagccgggc gccgcggtgg tggcgggacc ttatgtggcc
     1981 gagacctttg cggtgacggt cgaggacgcc tacgtcgtgg tcgagaccaa gtaggccgat
     2041 gcccccggcg atggctccct ggcgatggct ggggcccgag aacccggcca tggcgaggcc
     2101 tgcgcccgag cagggatcgc gtcgcccgcg ggtcacaccg cgcccaagct ctccggggcc
     2161 ggcgcaggct tcgcccctcg cctcttctgc attcttttca tctgcgctag gaatgcggcg
     2221 cctggagagc gggccaagct gagacactcg caaagcaaag ggaagaaacg catggctctg
     2281 atcgcaaggc tcggtattgt cgttgcggcg gccctggcgc tggcccagcc ggcggccgca
     2341 caggattttc ccaatcaccc cgtaaaaatc atcgtgccct tcggcgccgg cggccccgcc
     2401 gacgtgttcg cgcgcattat cgggcatcac ctccaggaag cgctcaagca atccttcatc
     2461 atcgagaacc ggcccggcgc aggggcagtg attggcacca atgaggtcgg caaatccgca
     2521 cccgacggct acacgctgtt gatgatgtcg aatacccaca cggtcaacga gaccttgctg
     2581 ccgaaccgga actatcagct gctgcgcgac ttcaccgccg tggcgccggt caactcctcc
     2641 gatctcttgc tggtcgtgca cccctccgtg cccgccaagg acttgaaaga atttctcgcc
     2701 ctgctcaaag ccaagcccgg cgagctcaac tacgcctcgt ccggcccggg cactccctac
     2761 cacatggccg ccgagctctt taagtccatg agcggcacca atctcgtgca cgtgccccat
     2821 cgcggcagtg gcgatgcgcg caccagtgtc atcggcggcc atgtgcagat gatgatcgat
     2881 gccatcacca ccatggcccc caccgcccgg gacggccagg tgcgcgcgct cggctcgacc
     2941 ggtcagaccc gctcctccgt catgccggac gtgccgacca tcgccgaggc cggtctgcca
     3001 ggttacgagg ccactatctg gctcggcatc atggcccccg ccggcacgcc aaagcccatc
     3061 gtcgataagc tcaatgccga gatcaacaag gttttatccc tgccggacct caaagcccaa
     3121 tgggcccagc agggcgcgca acccctgatc atgatgccgg ccgaattcga gtcctatctg
     3181 cgcaaggaca tcgagaagtg ggccaaggtc atcgagaccg ctggcatcaa ggtgcagcaa
     3241 tgacggccat ggctcccgag cccaccacgc tgatggtcaa tggggtcgag cgcacggtga
     3301 gcgccgacgc ggccacgccg ctcctcaatg tgctgcgcgc ggagctgggc ttgaccggta
     3361 cccgcttcgg ctgcggtgcc aatgcctgcg gcgcctgcaa cgtgatcatc gacggtgagg
     3421 ccgtggcctc ctgcgacacc cccttgtggg cggccgccgg caagcgcatc accaccatcg
     3481 agggcct
//
LOCUS       DASTMT010000334         2487 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_843651, whole genome shotgun sequence.
ACCESSION   DASTMT010000334 DASTMT010000000
VERSION     DASTMT010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2487)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2487)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2487
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_843651"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..314)
                     /locus_tag="VFR00_07790"
     CDS             complement(<1..314)
                     /locus_tag="VFR00_07790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012650118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU0159199.1"
                     /translation="MAVRRLGIVMHGVTGRMGSNQHLARSIGAICAEGGVLLGNGDRV
                     MPDPILVGRNADKLAELAKAHGFARVSDDLDAALANRNDTVFFDAATTQLRPTLLAKA
                     IAA"
     gene            complement(448..918)
                     /locus_tag="VFR00_07795"
     CDS             complement(448..918)
                     /locus_tag="VFR00_07795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006333490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1203 domain-containing protein"
                     /protein_id="HEU0159200.1"
                     /translation="MSVRFVALETPLVRRLQAGGADANGQTPEQHISPGRGIPCRHCL
                     QLVGEGEPYLILAHRPFPAPQPYAEQGPIFLHAAACARHAESEAVPAMLVSACYLLRG
                     YGRDDRIVYGSGQIVPTANIVTAAKALFADPGIAYIHVRSASNNCFQCRLQRAS"
     gene            1019..1444
                     /locus_tag="VFR00_07800"
     CDS             1019..1444
                     /locus_tag="VFR00_07800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006327135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1761 domain-containing protein"
                     /protein_id="HEU0159201.1"
                     /translation="MLNYFAVLLAGLASFAFGAAWYGALAKPWLAARGLAPDAPQPAP
                     RRLGPLPIAYLIAFFAELVMAFMLGGVLLHLAQGGLAPSLRTGAISAAFLWLGFVATT
                     LGVNHAFQGAKTTLTLIDAGHWLGVLLIQGAIIGAWGLR"
     gene            1574..>2487
                     /locus_tag="VFR00_07805"
     CDS             1574..>2487
                     /locus_tag="VFR00_07805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012110225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEU0159202.1"
                     /translation="MAEKIYNVPAEWTSRAWVDNSKYLAMYKRSVEDANLFWGEMGKR
                     LDWIKPYTRVKNTSYAPDNVSIKWYEDGVLNVSANCIDRHLKKRADQVAIIWEGDDPT
                     HDEKITYRQLYERVSKFANVLKANGVKKGDRVTIYLPMIPEAAYAMLACSRIGAVHSV
                     VFGGFSPDSLAGRIVDADSKFIITADEGVRGGRAIPLKKNTDEALKKCQGDEKVLVVR
                     RTGNEVPWSPGRDIWLHDELVKVGAECPPEPMQAEDPLFILYTSGSTGKPKGVLHTTG
                     GYLVYVALTHELVFDYHEGDIYWCTADVG"
BASE COUNT          440 a          812 c          815 g          420 t
ORIGIN      
        1 gcggcaatcg ccttggccag cagcgtggga cgcaattggg tggtggccgc gtcgaagaag
       61 accgtgtcgt tgcggttggc cagcgcggcg tcgagatcgt cgctgacgcg ggcaaacccg
      121 tgcgccttgg cgagctcggc aagcttgtcc gcgttgcggc ccacgaggat cggatcgggc
      181 atgacccggt cgccgttacc caacagcacg ccgccttcgg cgcaaatggc gccaatggaa
      241 cgggcaagat gctggttgct ccccatgcgc ccggtcacgc catgcatcac gatgccgagc
      301 cgtcgcactg ccatcacctg cctcccggat ccttgcccga gggccactta gggtgagcag
      361 ggagggcctg tcaatgtggg cggggccggg gcttcgcgcc gtcgcggcca caagccacgc
      421 tcccctgcct cgggcgccag gccgccgcta agacgcgcgc tgcagccggc actggaaaca
      481 gttgttggag gccgagcgca catggatgta ggcgatgccc ggatcggcga acagggcctt
      541 tgcggccgtc actatgttcg ccgtcggcac gatttggccc gagccgtaga cgatgcggtc
      601 gtcgcgccca tagccgcgca gcaaatagca ggcgctaacc agcatggctg gcactgcctc
      661 gctctccgcg tgccgcgcgc aagccgccgc gtgcaggaaa atgggaccct gctccgcata
      721 gggctgcggc gccgggaagg gacgatgcgc gaggatcaga tagggttcgc cttctccaac
      781 cagctgcagg caatgccgac agggaatgcc cctgcccggc gagatatgct gctccggcgt
      841 ctgaccattg gcgtctgcgc cgccagcctg caaccggcgc acgagcggcg tttcgagcgc
      901 gacaaagcgg acggacatgc gcatttcctc taagttgcag agcattcgcg ctcgcatagc
      961 ccgccaccaa ggcggccggc gacccgaaac ctgtgcactc ggtcaaggag acgcgacggt
     1021 gctcaactat ttcgctgtgc tgctggcggg ccttgccagc ttcgccttcg gcgccgcctg
     1081 gtacggcgcg cttgccaagc cgtggcttgc cgcccggggg cttgcgccgg acgccccgca
     1141 accagcgccg aggcgcctcg gccccctccc catcgcctat ctcatcgcct ttttcgccga
     1201 gttggtgatg gcctttatgt tgggcggtgt gctcttacac ctcgcgcagg gtggtctggc
     1261 gccgagccta cgcacgggcg cgatctcggc cgcgttcctg tggctcgggt ttgttgccac
     1321 cacgctcggc gtgaaccacg cctttcaggg cgccaagacc acgctcacgc tgatcgacgc
     1381 tgggcactgg ctgggcgtac tgctgattca gggcgccatc atcggcgcct ggggcctgcg
     1441 ctaacgcgag cgcggcgggg ccgcgccggt cgctcatagg ctcggcgcgg cgccgctcga
     1501 ccaaggagcc agttgcccat tgcgctgggg tgccgataat agagtgaaac gccagacctc
     1561 ccgggagaaa ccgatggccg agaaaatcta caacgtgccc gccgagtgga cgagccgcgc
     1621 ctgggtggac aacagtaaat acttggcgat gtacaagcgc tcggtggagg atgcgaacct
     1681 gttctggggc gagatgggca agcgcctcga ctggatcaag ccctatacgc gcgtcaagaa
     1741 cacctcctac gctcccgaca acgtctcgat caaatggtac gaggatggtg ttctcaatgt
     1801 ctcggccaac tgcatcgacc ggcatttgaa aaagcgcgcc gatcaggtgg cgatcatctg
     1861 ggagggcgat gaccccaccc acgacgagaa aatcacctac cgccagctgt acgagcgggt
     1921 ctcgaaattc gccaacgtgc tgaaggccaa cggcgtcaaa aagggggacc gggtcaccat
     1981 ttatttgccg atgatccccg aggcggccta cgccatgctg gcctgcagcc gtatcggcgc
     2041 cgtgcattcg gtggtgttcg gcggcttctc accggacagc ctggcgggcc gcatcgtcga
     2101 cgccgactcc aagttcatca ttacggcgga cgagggcgtg cgcggcggcc gcgccatccc
     2161 gctcaagaag aataccgacg aggctctgaa gaaatgccag ggcgacgaaa aggtcttggt
     2221 cgtgcgtcgc accggcaacg aggtgccctg gagccccgga cgggacattt ggctgcacga
     2281 cgagctggtc aaagtcggcg ccgagtgtcc gcccgagccg atgcaggcgg aagacccgct
     2341 ctttattctc tacacctcgg gctccaccgg caaaccgaag ggtgtattgc ataccactgg
     2401 cggctacctg gtctatgtcg ccctcacgca cgaactggtg ttcgactatc atgagggcga
     2461 tatctactgg tgcacggccg acgtcgg
//
LOCUS       DASTMT010000335         4102 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_2157755, whole genome shotgun sequence.
ACCESSION   DASTMT010000335 DASTMT010000000
VERSION     DASTMT010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4102
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2157755"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(53..379)
                     /locus_tag="VFR00_07810"
     CDS             complement(53..379)
                     /locus_tag="VFR00_07810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0159203.1"
                     /translation="MQSTKLAMIAALGLASMGLGTLAAAAAPTAPGPAAVTAGTDGKA
                     ESIAYRRCWWQGGHRHCRWFGPADRGYGYGYRYYGPLYPEAYRTGTRRWWQEMDRDDR
                     GGRGRP"
     gene            complement(601..1962)
                     /locus_tag="VFR00_07815"
     CDS             complement(601..1962)
                     /locus_tag="VFR00_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018898630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HEU0159204.1"
                     /translation="MLNKLDRDLQGVLAGDAKGWRRARGDAALTEVNRSISVRGATVG
                     RRLVAFMGPGYLVAVGYMDPGNWATSLAGGSKFGYTLLVVALISNIMAILLQALCARL
                     AIASGRDLAQACRDAYPPWAAYPLWVAAEIAIIATDIAEVIGTAIGLNLLFHIPLEIG
                     VLLTALDVFLILYLQKLGFRYLEAFIITLLGVITACFAIQIVLADPELGPLLRGFAPT
                     TEIVRNPEMLYLAMGILGATVMPHNLYLHSAIVQTRAYGETLPAKREALKYATIDSTI
                     ALMFALLINAAILVLAAASFHKAGHTEVVELGQAQALLAPLLGSSLAPMLFGLALLCC
                     GLSSTVTATLAGQAVMEGFLDIRLPGWLRRLITRAIAIIPAAGVTLLYGEAETGKLLI
                     LSQVILSLQLPFAVVPLVQFTADKAKMGVLVAPRWLVACAAAIAAVIIALNLKLLWHQ
                     FIG"
     gene            complement(2202..3416)
                     /locus_tag="VFR00_07820"
     CDS             complement(2202..3416)
                     /locus_tag="VFR00_07820"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011157857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HEU0159205.1"
                     /translation="MAATVQDPIVIVGMARTPIGAFQGKLAPLSAPQLGSAAISCALA
                     RAGVAAEDVSEVLMGCVLPAGLGQAPARQAALGAGLPTAVPATTVNKMCGSGMKTVML
                     AYEGLHARPDDIIVAGGMESMTNAPYLLPKLRGGARLGHTEVKDHMFLDGLEDAYDKG
                     RLMGSFAEDCAEQYQFTREAQDAFATESLRRARQANEDGSFAAEIVPLSVRGRTGELE
                     VAQDEQPFSADLAKIPKLKPAFREGGTVTPANSSSISDGAAALVLMREGVALRRKLQP
                     LARVVGVASVAQAPALFTTAPVAAIKTLLQRVGWSSAEVGLYEINEAFAVVSMAAMRD
                     LALPHQRVNVHGGACALGHPIGASGARILVTLLAAMRKYGQARGVAALCIGGGEATAV
                     AVEGFAPAARAA"
     gene            complement(3541..>4102)
                     /locus_tag="VFR00_07825"
     CDS             complement(3541..>4102)
                     /locus_tag="VFR00_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019400111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEU0159206.1"
                     /translation="QKLDKLGMRGSSTGELVFEDCEVPAENVLGAVGRGVNVLMSGLD
                     YERAVLAAGPIGIMQSALDVVIPYVHQRKQFGEPIGSFQLVQGKLADMYTTMNACRAY
                     VYAVAKACDRGETARKDAAGAILYAAEKATQIALDAVQLLGGNGYINDFPTGRLLRDA
                     KLYEIGAGTSEIRRWLIGRELFEETR"
BASE COUNT          731 a         1375 c         1356 g          640 t
ORIGIN      
        1 ggcctaaagc cacgccccgt cgcagaaaag ctaaggtgtt cgggtcgaag cgtcaggggc
       61 gcccccgacc tccgcggtcg tcgcggtcca tctcctgcca ccagcggcgc gtcccggtgc
      121 gataggcttc cggataaagc ggcccatagt agcggtagcc atagccatag ccgcgatcgg
      181 ccgggccaaa ccagcggcaa tggcgatgac cgccctgcca ccagcagcgc cgataggcga
      241 tgctctcggc tttgccgtcg gtacccgcgg tcactgccgc cgggccaggc gccgtcgggg
      301 cggctgcggc ggcgagcgtg cccagcccca tgctggcgag gcccaaggcg gcgatcattg
      361 ccagttttgt gctttgcatc gttcttcctt tccgttcttg ttgagtaaac gccgcccaaa
      421 atggcgggtt cctcgccggc gttgaggcgc acgcgccccg atcccgacat gagcaacgca
      481 gagcttgccc gcaacccgtc gctcgaaatt ggcgcgggcg cgtgggcgag ttgagacgcg
      541 cgctgagcgc gccgggccaa ggctgctagg gcgcggcaca aatcgaccgc gcttgcccgc
      601 tcagccgata aactgatgcc acaggagctt gagattgagc gcgatgatga ctgccgcaat
      661 cgcggcggcg caggcaacaa gccagcgcgg ggcgacgagc acccccattt tggctttgtc
      721 ggccgtgaat tgcacgagcg gcacgaccgc gaacggcagc tgcagactga gaatgacttg
      781 actgaggatc aagagcttgc ccgtctctgc ctcgccgtag agcagcgtga cgccggcggc
      841 aggaatgatg gcgatggcgc gtgtgatcag gcggcgcagc cagcccggca ggcgaatatc
      901 gagaaagccc tccatcacgg cttgcccagc cagggtcgcc gtcaccgtgg aactgaggcc
      961 gcagcagagc agcgccagcc cgaacagcat cggggccaaa gacgagccaa gcagcggcgc
     1021 cagcagcgcc tgggcctgtc ccaactcgac gacttccgtg tgaccggcct tgtggaagct
     1081 cgcggccgcc aggaccagga tggcggcatt gatcagcagg gcgaacatca aggcgatggt
     1141 ggaatcgatg gtcgcatact tgagggcctc gcgcttggcc ggcagcgtct cgccataggc
     1201 gcgggtctgc acgatcgcgg agtgcaggta gaggttgtgc ggcatcaccg tggcgcccaa
     1261 aatgcccatc gccagataga gcatttccgg attgcgcacg atctccgtgg tcggggcaaa
     1321 cccgcgcagc aacggcccca actcggggtc ggccagcacg atttggatgg caaagcaggc
     1381 ggtgatgacg ccaagcaagg tgatgatgaa ggcttcgaga tagcgaaagc cgagcttctg
     1441 caaataaagg atgagaaaga catcgagtgc cgtcagcagc acgccgattt cgagcggaat
     1501 atgaaacagc aaattgaggc cgatggccgt gccgatcact tcggcgatgt cggtggcgat
     1561 gatggcaatt tcggccgcga cccatagcgg ataggccgcc cacggtggat aggcgtcgcg
     1621 gcaggcctgc gccaaatcac ggcccgaggc gatcgccagg cgcgcacaca gcgcctgcag
     1681 caggatcgcc atgatgttgg aaatcagcgc gacgaccaag agcgtgtaac cgaacttgga
     1741 gccgccggcc aaggaggtcg cccagttgcc ggggtccatg tagccgaccg cgaccagata
     1801 gccgggaccc atgaaggcaa ccaagcgccg cccaaccgtc gccccgcgca cggagatgct
     1861 gcggttgacc tcagtcagag cggcatcacc gcgggcgcgg cgccaacctt tggcgtcacc
     1921 tgcaaggacc ccttgcagat cccgatcgag cttattgagc atgggccgct ccggcacccc
     1981 tccacatcag cggtgccacc atacccttct tgcaaatcat tcgcaagaag agatcggatc
     2041 ggcgccccgc gggctcggac gaatgcgcgg ggtcgctaca actttttgcc ggtcgtgcgg
     2101 caccggcgga tattgccatc gccagcgccc tagcggcgag ccgccgcagc gctcacggct
     2161 gacccgcacc gacgcccctg cggcctggcg ccggcacaat cttaagccgc gcgcgcggcc
     2221 ggggcaaagc cctcgacggc aaccgctgtt gcctcgcccc cgccgatgca gagcgcggca
     2281 acgccgcgcg cctgaccata cttgcgcatc gcagcgagca gggtgacaag gatgcgggcg
     2341 cccgaagcgc cgatgggatg gcccagcgca cacgccccgc cgtgcacgtt gacgcgctga
     2401 tgcggcaggg caaggtcgcg catggcagcc atgctgacga ccgcgaaggc ctcgttgatt
     2461 tcgtaaaggc ccacctcggc actcgaccag ccaacccttt gcaacagggt tttgatcgcc
     2521 gccaccggag cggtggtgaa tagggcgggc gcctgggcca cgctggcgac cccgacaacc
     2581 ctggccaagg gctgcagttt gcggcgcagc gctacccctt cccgcatcag cacgagggcg
     2641 gcggccccat ccgaaatcga actggaattg gcgggtgtca cggtgccacc ctcacggaag
     2701 gccggcttca gcttggggat cttggcgaga tctgcgctga agggttgttc gtcttgggcg
     2761 acctccagct cgcccgtgcg cccgcgcacg ctcagcggca cgatttcggc ggcaaacgag
     2821 ccatcctcgt tggcttggcg cgcgcggcgc aagctctcgg tggcgaaggc atcctgggcc
     2881 tcgcgcgtga actgatactg ctcggcacaa tcctccgcaa agctgcccat cagccggccc
     2941 ttgtcgtagg catcctccag gccgtcgaga aacatgtgat ccttgacctc ggtatgcccg
     3001 agccgggcgc cgccgcgcag cttggggagc aagtagggcg cgttggtcat gctctccatc
     3061 ccgccggcga cgatgatgtc gtcggggcgc gcgtgcagcc cctcataggc cagcatcacc
     3121 gttttcatgc ccgacccgca catcttgttg acggtggtgg cggggacggc tgtgggcaat
     3181 ccagcgccga gcgcggcctg gcgggcgggc gcctgcccga gcccggcggg cagcacgcat
     3241 cccatcaaga cctcgctgac gtcctcggcg gctacaccgg cgcgggccag cgcgcaactg
     3301 atggcggcgc tgcccagctg gggcgcgctg agcggcgcaa gttttccctg aaaggccccg
     3361 atcggcgtgc gcgccatgcc gacgatgacg atcgggtcct gaaccgtggc tgccataggg
     3421 gtgctcgcat tttcttacag tgtgtcgtcg tccgcgccat cgaacaccgc gccgagagcc
     3481 gggtcaatgc ggccggggca cgaagcgcgt gcgccggctc gagtgccggg cgcgcggcgc
     3541 ctatcgcgtc tcttcgaaca gctcgcgccc gatcagccag cggcggatct cgctggttcc
     3601 cgcgccgatc tcgtagagct ttgcatcgcg cagcaagcgg ccggtcggga agtcattgat
     3661 gtagccgttg ccgcccaaca gctgcaccgc atcgagcgcg atctgcgtcg ccttctcggc
     3721 ggcatagagg atggcccccg cggcgtcctt gcgcgcggtc tcgccccgat cgcaggcctt
     3781 ggcgacggca tagacgtagg cgcgacaggc attcatggtg gtgtacatgt cggcgagttt
     3841 gccctgcacc aactggaagc tgccgatcgg ctcgccgaat tgcttccttt gatgcacgta
     3901 aggtatcacc acgtccaagg ccgattgcat gatgccgatc ggacccgccg ccagcacggc
     3961 gcgctcgtag tcgaggccgc tcatcagcac gttgacgccg cgccccaccg cgcccagcac
     4021 gttctcggcc ggcacttcgc agtcctcgaa caccagctcg ccggtcgacg agccgcgcat
     4081 gccgagcttg tcgagcttct gg
//
LOCUS       DASTMT010000336         2736 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_5346940, whole genome shotgun sequence.
ACCESSION   DASTMT010000336 DASTMT010000000
VERSION     DASTMT010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2736
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5346940"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            154..1218
                     /locus_tag="VFR00_07830"
     CDS             154..1218
                     /locus_tag="VFR00_07830"
                     /inference="COORDINATES: protein motif:HMM:NF014045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEU0159207.1"
                     /translation="MEVLDALLVEGKRVLFSSAPAALFFWFNLAFLIALGCLLLPGRL
                     RKARRRREIQRLFEASTRASRGLRRDNELAALARHLGRLAEGFGSRLGQVRAERIQLA
                     KVYIDHSNFTKNWKRVINNDVHSIERDIIWSKLPELLLAETRRWLAESRKEPPALVYR
                     GTNVYGTLFEEEYFELLDNMLRLERSAPEKLPLKIYFQKETVERWRQENEAQRTELAQ
                     LQNEFGFLVFTIARRTPRLDRLGSSNFTVGGIPIAPEKQLDTLVTTDLIADAMYDVYD
                     IAFLISEDSDFCPAVEFVQDTRNKHVVHVGFGSHSNDLRSKCRLRIDLAGKDQLYRRM
                     QAKPAGEAVPARAKADNGGG"
     gene            complement(1224..2342)
                     /gene="ald"
                     /locus_tag="VFR00_07835"
     CDS             complement(1224..2342)
                     /gene="ald"
                     /locus_tag="VFR00_07835"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014131201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HEU0159208.1"
                     /translation="MRIGVPKEIKVHEYRVGMAPGSVREAVAHGHQVFVERDAALRQG
                     ISDRDYERAGATVLGTAAEVFAKADLIVKVKEPQPSEFALLRAGQTLFTYLHLAPDPE
                     QAKALLAAGGTAIAYETVTSPRGGLPLLAPMSEVAGRMAVQAGAHCLEMERGGRGMLL
                     GGVAGVAPAKVVVLGGGVAGVNAVRMAIGLEAQVTVLDINVERLYAIDQQFGAAVNTI
                     FSTRQAVEDYLIEADLVIGAVLVPGAAAPKLVTEQHVKNMQRGSVLVDISIDQGGCSE
                     TSRPTTHAEPTYVVHDVVHYCVTNMPGAVARTSTFALNNATLPFILALANKGPQRALL
                     EDPHLRDGLNIMQGHVVHPVVADALGLAHRDARELLKG"
     gene            complement(2386..>2736)
                     /locus_tag="VFR00_07840"
     CDS             complement(2386..>2736)
                     /locus_tag="VFR00_07840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013949418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HEU0159209.1"
                     /translation="SEEPRKLLRNAGFTLMEIPEGHICCGSAGTYNVLQPELAADLRA
                     RKVDHIKSVRPDAVAAGNIGCIVQLAHGMEIPIAHTVELLDWAYGGPVPRGLEGLADQ
                     VEEVPEPKLLLAQP"
BASE COUNT          454 a          913 c          895 g          474 t
ORIGIN      
        1 aaaactgcca gcccaggccc aattggcggg cgttgcggcc gggggattct tgtcccttgg
       61 cgacggaccg cttagcctca cctacaacat tacaggtgcg gcgcggacgt ggtgcaggcg
      121 gcgttcgcaa ggccgtgtga gggtaggggt ggcatggagg tgctcgacgc gcttctggtt
      181 gagggcaagc gcgttctgtt ctccagcgct ccggcggcgc tcttcttctg gttcaatctc
      241 gcctttttga tcgcgctcgg ctgcttgttg ctgccgggcc gcctgcgcaa ggcgcggcgc
      301 cggcgcgaaa ttcagcgtct gtttgaggcc tcgacacggg ccagccgcgg cttgcgaagg
      361 gataacgagc tggccgcgct cgcccgccac ctcggccgtt tggccgaggg cttcggcagc
      421 cggctcgggc aggtgcgcgc cgagcgcatt cagctggcca aggtctacat cgaccattcc
      481 aacttcacga aaaactggaa gcgcgtcatc aacaacgacg tccacagcat cgagcgcgac
      541 attatctggt cgaagctgcc ggagctcttg ctggcggaga cccgtcgttg gctggcggaa
      601 agccgcaaag agccgcccgc gctcgtctat cgcggcacca acgtctatgg caccctgttc
      661 gaagaggaat atttcgagct gctcgacaac atgctgcgcc tggagcggtc ggcgccggaa
      721 aaacttcccc tcaagatcta tttccagaag gagacggtgg agcgttggcg ccaggagaac
      781 gaggcgcagc ggaccgagct cgctcagctg cagaacgaat tcggcttctt ggtgttcacg
      841 attgccaggc gcacgccacg cctcgatcgc ctggggtcga gcaacttcac ggtcggaggc
      901 atcccgatcg cacccgaaaa gcagctcgat acgctggtaa cgaccgatct cattgccgat
      961 gccatgtacg acgtctacga catcgccttc ctaatttcgg aggacagcga cttctgcccg
     1021 gccgtggagt tcgtgcagga cacgcgcaac aagcacgtcg tgcatgtcgg tttcggctct
     1081 cacagcaacg atttgcgttc caagtgccgc ttgcgcatcg atcttgccgg caaggatcag
     1141 ctctaccgcc gcatgcaggc caagcccgcg ggcgaggcgg tgccggcgcg cgccaaggcg
     1201 gacaacggcg gcggctgagg cgctcagccc ttgagcaact cgcgcgcatc cctatgcgcg
     1261 aggccgaggg cgtccgccac cacgggatgc acgacatggc cttgcatgat gttgagaccg
     1321 tcccgcaggt gcggatcctc cagcagcgcg cgctgcggcc ctttgttggc gagcgccagg
     1381 atgaagggca gggtcgcgtt gttgagcgcg aaggttgagg tgcgcgccac cgcgcccggc
     1441 atattcgtca cgcaatagtg cacgacgtcg tgcacgacat aggtcggctc ggcgtgagtg
     1501 gtcgggcgcg acgtttccga gcagccgccc tggtcgatcg atatgtcgac gagcacggag
     1561 ccgcgctgca tatttttgac gtgctgctcg gtgacgagtt tgggcgcggc ggcgccgggc
     1621 accagcacgg cgccgatcac caggtccgcc tcgatcagat agtcctccac cgcctggcgc
     1681 gtcgagaaga tggtgttgac cgcggcgcca aactgctgat cgatggcata gaggcgctcg
     1741 acgttgatgt cgagcaccgt cacctgcgcc tccaggccga tcgccatgcg cacggcattg
     1801 acgccggcga cgccgccgcc caggaccacc accttggcgg gggcgacgcc ggcgaccccg
     1861 cccaacagca tgccgcggcc gccgcgctcc atttccaggc agtgcgcgcc cgcctgcacg
     1921 gccatgcggc cggcgacctc gctcatcgga gccagcaagg gcaggccgcc gcgcgggctc
     1981 gtcaccgtct cgtaggcgat cgccgtgccg cccgccgcca gcagggcttt ggcctgttcg
     2041 ggatcgggcg ccagatgcaa ataggtgaag agcgtctgtc ccgcgcgcag cagggcgaac
     2101 tcggagggct gtggctcctt caccttcacg atcaggtcgg ccttggcgaa gacctccgcc
     2161 gccgtgccga gcacggtggc tcccgcgcgc tcatagtcgc gatcactgat gccctgacgc
     2221 agggcggcat cgcgctcgac gaagacctga tgcccgtgcg ccaccgcctc gcgcacgctg
     2281 cccggcgcca tgccgacgcg atactcgtgc accttgattt cctttggcac gccaatgcgc
     2341 atcaccatcg ctcccgagcc cgctggggct ctttttgccc gaagctcacg gctgagccag
     2401 cagcagctta ggctccggca cctcttccac ctgatcggcc aagccttcga gcccgcgcgg
     2461 cactgggcca ccataggccc agtcgaggag ctcgacggta tgggcgattg gaatctccat
     2521 gccgtgggcg agctgcacga tgcagccgat gttgcccgcc gccaccgcgt cggggcgcac
     2581 gctcttgata tggtccacct tgcgggcgcg caggtcggcc gcgagctcgg gttggagcac
     2641 attgtaggtc ccggccgaac cgcagcaaat atggccttcg gggatctcca tcagcgtgaa
     2701 gccggcgttg cgcaacagtt tgcgcggctc ctcgct
//
LOCUS       DASTMT010000337         9849 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_2811109, whole genome shotgun sequence.
ACCESSION   DASTMT010000337 DASTMT010000000
VERSION     DASTMT010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 9849)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9849)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9849
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2811109"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(48..791)
                     /locus_tag="VFR00_07845"
     CDS             complement(48..791)
                     /locus_tag="VFR00_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012320012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1194 domain-containing protein"
                     /protein_id="HEU0159210.1"
                     /translation="MARHMTTIVVALAVAIGIWSRHPAAAEDGQVDLLLVLAADISRS
                     IDEKKFRLQRDGYAAAIVDPRVVRAMQAGASGRIAICYMEWASDQDQRVIVEWTRVGN
                     EGEAKEVADRIREAPRAFMGRTAIGAAIDFSMGLLSRSPFGAPRQIIDVSGDGTNNSG
                     RDVTVARDAAIERGVIINGLVILSEIPLPTNPMHTHPPGGLTAYYENNVIGGPGAFVL
                     EAQNFESFGQLLIQKLIKEIAGLRAPTRG"
     gene            complement(975..1388)
                     /locus_tag="VFR00_07850"
     CDS             complement(975..1388)
                     /locus_tag="VFR00_07850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0159211.1"
                     /translation="MRSSVLMVTLLLAAVAGPATAQLPFGTPPLPESGLRRQCTKDYR
                     ASLEAQITAMEKMRTAGPEFVGEICSMIEQGSALIGGELPDSVRQQLKGLLGFDVDLR
                     FIKTQCRIGQGNLDRELTTQLGFLKSEIVRCNDSI"
     gene            1616..3124
                     /locus_tag="VFR00_07855"
     CDS             1616..3124
                     /locus_tag="VFR00_07855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004129823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HEU0159212.1"
                     /translation="MLDRPAKLKAYQTFIDGNWSDAASGKSFETFNPYTGEAWATIPE
                     CDKVDVDRACQAAARAFEVGPWPAMSATARGKIMRRIAGLIEKHAQHLAEIEVRDNGK
                     LMSEMHAQTKYLPEWFYYYGGLADKIQGAVVPADRPDHFNYILREPIGVCAFITPWNS
                     PLMLVGWKLAPALAAGCTVVLKPSEFTSASLLEFIKLVIEPAELPPGVVNVVTGYGAV
                     VGEPLVTHPKVAKVAFTGGTVTGARVNELAAKSFKKVSLELGGKSPNIVFDDCNLEDA
                     VSGAVSGIFAATGQTCIAGSRLLLQDTIHDRFVEKLVAMAKMARLGDPGQLETQVGPV
                     TTPAQYQKILSYIDIAKGEGATCVLGGGPAGKEDGGGKYFVKPTIFTGVNNAMRIAQE
                     EVFGPVLSVIKFKDEEEAVKIANDIAYGLGAGIWTQSLKRATTMAKRIRAGTVWVNTY
                     RAVSFTMPFGGYKASGLGRENGMEAIDGYLQSKSVWINNGPGGGNPFIMKTS"
     gene            3396..4391
                     /locus_tag="VFR00_07860"
     CDS             3396..4391
                     /locus_tag="VFR00_07860"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012168794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEU0159213.1"
                     /translation="MPYNSFGVIGGGAWGTALGQTLRQAGRDVVLWARELEVVEEINA
                     EHRNTPFLPGVTLDAGLRATGELADVARQDVVLMVAPAQHVRPLAAELAGQLAAGKPV
                     VICAKGLERATAKLLGEVLSETLPQAVQAVLSGPSFAADVARGLPAALSLASVQEDLG
                     RSLAAAIGYRHLRIYWSDDPVGVQLGGAVKNVLAIAAGIVEGKGFGASAHAALVTRGF
                     AEMRRFGAALGARPDTLMGLSGLGDLLLTCGSPQSRNMSLGRALGQGQILQQVLGNRR
                     SVAEGVYTAAAVVRAAAREGIEMPISEAVHAIVAGSIGTDTAIEALLSRPQRAES"
     gene            complement(4457..6121)
                     /locus_tag="VFR00_07865"
     CDS             complement(4457..6121)
                     /locus_tag="VFR00_07865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEU0159214.1"
                     /translation="MTTIVPAGPGQDARLLEACGDGLNAADRVFHLAKAGVKAAAAEA
                     QEAEAAQAALHGLAWLATYVAALRQMLGWGRHLLATHRFGEAERLLLTGAFGEYLAQI
                     AGGIPMSQNETVRLSELGVARAEARRFEDDVAGLIDQGTDRAVRARLAALIAEQPGLT
                     TFGDTGLDETLTEMRSQMRRFAEAEVLPHAHQWHLKNAYIPSEVVSKLGELGVFALTL
                     PEQFGGLGLGKEAMCVVSEELSRGYIGVGSLGTRSEIAAELILGAGTEAQKQKYLPRI
                     ASGALLPTAVFTEPNTGSDLAALKTRAVREGDVYKVTGQKTWITHPVRADLMTLLVRT
                     DPRLPGYKGLSMLLAEKPRGDDADPFPAAGMSGGEIEVLGYRGMKEFDISFDAFEVPT
                     AQLLGGVEGQGFKQLMTTFEAARIQTAARAIGVAQSAMDLALAYALGRQQFGKAIFAF
                     PRVANKIVMMAIETMIARQLTYHAAREKDRGRRCDLEAGMAKLLAARIAWANADNAVQ
                     IHGGNGFALEFPVSRLLCDARILNIFEGAAEIQAQVIARRLLEGAN"
     gene            complement(6215..6889)
                     /locus_tag="VFR00_07870"
     CDS             complement(6215..6889)
                     /locus_tag="VFR00_07870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0159215.1"
                     /translation="MSVDEESKLVALRRARERLEVALAADESWQTLQRLGAATPGRDQ
                     EAGSLELRLQSSPIYRAWKNLNAAIEARQRDGAGSAGAAPAAQEIPGHAPERTLPGGE
                     TTAPGRPDATARPASVAPAPGAASTSGGAPNVPSRVLPAEAEASVSFVARTPTPARWQ
                     PSATASSTQRWVGVDGQGGWSALPESQALRDEEAEVSVVSVDALNHAGAVQRLLRALR
                     GETNQG"
     gene            7139..7975
                     /locus_tag="VFR00_07875"
     CDS             7139..7975
                     /locus_tag="VFR00_07875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEU0159216.1"
                     /translation="MFSRHLKPVAIAFHETFADSGFSMAGAVAYSFVLSLFPFCIFLA
                     ALAGYFGGEALAEAAVQQLFQLAPAPVVEAIAPEVMRVMGQSRFGLLTVGALIALFFA
                     TSAVESLRAALNIAYRVKEKRSYLRCMAQSALFVVLSAVGMLALTWGVVVGPQVAAGL
                     KPSWLLWLTDQGWTAFIGRYGIVIIAIGGQLLAYHLWLAAGSRGLGEVLPGVLLSLLL
                     WIAAARLFGSWLSISDYSRFYAGLTQIMSALVFFQLSAVVVILGAELNRGILEMRRRL
                     AE"
     gene            complement(8005..8808)
                     /locus_tag="VFR00_07880"
     CDS             complement(8005..8808)
                     /locus_tag="VFR00_07880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEU0159217.1"
                     /translation="MNKSLVQEQFGAHAAAYVTSTVHAKGASLARLVELVQPQKQWQA
                     LDVATGAGHTALAFAPHVASVIASDLTQQMLDEAAKLAEIKGIANLSTALADAEALPF
                     EDARFDLVTCRIAPHHFPDIPSFVAEVERVLKPGGTFALADNIEPDAESTPGFVPIEL
                     RDAALIYNTFERLRDPSHSRCLGMTEWGELIEDAGLAVIHKERAPKEMEFEPWATRMG
                     CSAPTVERLRSILLEASPALKAFLKPRSEGGALFFTLDEAVIIARKPAA"
     gene            complement(9107..9523)
                     /locus_tag="VFR00_07885"
     CDS             complement(9107..9523)
                     /locus_tag="VFR00_07885"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF025008.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEU0159218.1"
                     /translation="MSGLTREAANALLADNFAPWVLASGLKVETIVADGVVLRLPFAA
                     ELCRTGGTLCGQALISGADSAMVLALAAANGAFKPCTTIDLSISYMRPISQADALLEA
                     KLMRLGKTLAFCSCAVSAAGTDKPAAFATGTYALLG"
     gene            complement(9654..>9849)
                     /locus_tag="VFR00_07890"
     CDS             complement(9654..>9849)
                     /locus_tag="VFR00_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012253007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016531 - copper chaperone activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HEU0159219.1"
                     /translation="PGASVVLKPFAHHLMMMDLKQPLQAGQTIKGTLVFEKAGSVEIT
                     YVVVPMGADGPGAGARARSK"
BASE COUNT         1591 a         3234 c         3267 g         1757 t
ORIGIN      
        1 tgccgcggtg cggatttcac tgcggtcaat tgagtccaca ccctagttta gccgcgcgtg
       61 ggtgcccgca ggcccgcaat ttctttgatc agcttttgaa tgagcagttg cccgaaagat
      121 tcgaaattct gcgcctccaa gacgaaggcg ccggggccgc cgatcacgtt gttctcgtag
      181 taggccgtca ggccgccggg cggatgggtg tgcatgggat tggtagggag aggtatctcg
      241 ctcaggataa cgagcccatt gataatgacg ccgcgctcga tggcggcgtc gcgggcgacc
      301 gtcacgtcgc gcccagaatt gttggtgccg tcgccggaga catcgatgat ctggcgcggt
      361 gccccaaacg gactgcgcga caaaagaccc atgctgaagt caatggccgc gccgatcgcc
      421 gttcgcccca tgaatgcgcg cggcgcctca cgaatgcgat cggcaacctc cttggcttcg
      481 ccctcgttgc ccacccgcgt ccattcgacg atcacccttt gatcctgatc gctcgcccat
      541 tccatgtagc agatggcgat gcgccccgag gcgccggcct gcatggcgcg caccacacga
      601 ggatcgacaa tggcggcggc gtagccgtcg cgctgcagac gaaatttctt ttcatcgata
      661 ctccgggaga tgtcggcggc cagcaccagc aacaaatcga cttggccgtc ctccgccgcg
      721 gccgggtgcc gcgaccaaat accgatggcg accgccagcg cgacaacgat cgtcgtcatg
      781 tggcgtgcca tgacgcctct cctgatcgat accggacctc gacgacagac taggccagaa
      841 ggctcaagtc ggtcgagcct tcttcggcag gccgcgtttt cgcaagctcg ggaggggtgc
      901 tccccccttg cgtcttgccg ccgctggctg ctgcaagctg tggcctgccc gtcggccgca
      961 cacggtgcgg cgggctaaat gctgtcgttg cagcggacaa tctcggactt gagaaagccg
     1021 agctgggtcg tcagctcgcg gtcgagattg ccctggccga tccggcactg ggttttgatg
     1081 aaccgcaggt cgacgtcgaa gccgagcagg cccttgagct gttggcgcac gctgtcgggg
     1141 agttcgccac cgatcaaagc gctgccttgc tcgatcatgc tgcaaatctc gccgacgaac
     1201 tcgggcccgg ccgtgcgcat tttctccatg gcggtaatct gcgcctccag cgaggcgcgg
     1261 taatccttgg tacactgccg ccgcaacccg gattccggca atgggggtgt gccgaacgga
     1321 agctgcgcgg tcgccggtcc ggcgactgcc gcaagcagca gcgtgaccat gaggacagaa
     1381 gagcgcatgc caacctcccg ttcccggcga agccgctgac gacccctgcc acaagggagc
     1441 gcgcggacca tgacccggcg cgagcagatc ccacatgttt gtcgggatgt gggcggagcc
     1501 attacggcgc ggtaatgtcc gccctcagca atgtccggcg cggcttgaac ccctagccgc
     1561 cgcgcactag ccttcctcag atctaaatgc tcgcagtcag gtcccggaga gtgccatgct
     1621 agatcggccc gccaagctca aagcctacca aaccttcatt gacggcaact ggtcggatgc
     1681 cgcgtccggc aagtcgtttg agaccttcaa tccctatacg ggcgaggcct gggccacgat
     1741 cccggaatgc gacaaagtcg acgtcgatcg tgcctgccag gcggccgcgc gcgccttcga
     1801 ggtgggacca tggcctgcca tgtcggcgac ggcacgcggc aaaatcatgc gccggattgc
     1861 cggactgatc gaaaagcatg cccaacatct ggccgagatc gaagtacgtg acaacggcaa
     1921 gctgatgtcg gagatgcacg ctcagaccaa gtatctgccc gagtggttct actactacgg
     1981 ggggctcgcc gataaaatcc aaggtgccgt ggtgcccgcc gaccggccgg accatttcaa
     2041 ttacatcctg cgcgaaccca ttggcgtatg tgccttcatc acgccgtgga actccccgct
     2101 catgctggtg ggctggaaat tggcgccggc gctggcggcc ggctgcaccg tggttttgaa
     2161 gccctcggag tttacctccg cgtcgctgct cgaattcatc aaactggtga tcgagccggc
     2221 cgagttgccg cccggcgtgg tcaacgtcgt aactggttat ggcgccgtcg tcggcgagcc
     2281 gctggtgacg catcccaagg ttgccaaagt tgccttcacc ggcggcacgg tcacaggcgc
     2341 gcgtgtcaat gagctcgccg ccaagtcctt caagaaggtg tcgctggagc tcggcggcaa
     2401 gtcgcccaat atcgtgttcg atgactgcaa cctcgaggat gccgtgagcg gtgccgtctc
     2461 cggtatcttc gcggcaaccg gccaaacctg catcgccggc tcgcggctct tgttgcagga
     2521 cacgatccat gaccgcttcg tggagaagct ggtggcgatg gcgaagatgg cgaggctcgg
     2581 ggatccaggc cagctcgaga cccaggtcgg cccggtgacg acgcccgcgc agtaccaaaa
     2641 gattctgtcc tacatcgata tcgccaaggg cgagggtgcc acctgcgtcc tcggcggcgg
     2701 tcccgcgggc aaggaggatg gtggcggcaa atacttcgtc aagcccacga ttttcacggg
     2761 tgtcaacaat gcgatgcgca tcgcgcagga agaggtcttt ggcccggttc tttccgtcat
     2821 caaattcaag gacgaggagg aggccgtgaa gatcgccaac gatatcgctt acggcctcgg
     2881 tgccggtatc tggacccagt cgctcaaacg cgcaacgacc atggcaaaac gcattcgcgc
     2941 cggcacggtc tgggtcaaca cctaccgcgc cgtgagtttc accatgccgt ttgggggcta
     3001 caaggcgtcc ggcttgggtc gcgagaacgg catggaagcg atcgatggat acctgcagtc
     3061 gaagagcgtg tggatcaaca acggtccggg cggcggcaac cccttcatca tgaagacatc
     3121 ataaaggatc atgaggacga ggagctcgct tggcgaggct gatgctccgt gagactaacg
     3181 cggtcgaggg ccgctgtggc cggcgcggtt gccggacgca caagcctcgg gcgcgcgggc
     3241 gaggttccgc gcgggccccg agcggcgatg gctgatcgcc tggtcggcgc ggcggcggag
     3301 ccggttgggc aatctcgcgg gtcgccggcg ggcggccgga tcgacaatca gggcgccggc
     3361 ggctggggtc gggcgccaca gcaaggatgg caggcgtgcc ctacaacagc ttcggggtca
     3421 tcggcggcgg tgcgtggggg accgcgctcg ggcagacgct gcggcaggcc ggccgcgatg
     3481 tggtgctatg ggcgcgcgag ctggaagtcg tcgaggaaat caacgctgag caccgtaaca
     3541 cgccgttcct gcccggcgta acacttgatg ccggtttgcg cgcgaccggc gagctggcgg
     3601 atgtggcccg gcaggacgtg gtgctgatgg tggcgccggc tcagcatgtg cgccccctcg
     3661 ccgccgagct ggcgggccag ctggccgcgg gcaagcctgt cgtcatctgc gccaagggac
     3721 tggaacgggc gacggcaaag ctattgggtg aggtgctgag cgagacgttg ccgcaggccg
     3781 tgcaggccgt tctttccgga ccgagcttcg cggcggacgt cgcccgtggc ctgccggccg
     3841 ccctcagcct tgcctctgtg caggaagatc tggggcggtc gctggccgcc gccattggct
     3901 atcgacattt gcgcatttac tggagcgatg acccggtcgg tgtacagctc ggcggtgccg
     3961 tcaagaatgt gctggcgatc gccgctggca tcgttgaggg caaaggtttt ggcgccagtg
     4021 cgcatgccgc cttggtcacc cgtggcttcg ccgagatgcg ccgtttcggg gccgccctgg
     4081 gtgccaggcc ggacacgctg atgggcctat cggggctcgg cgatctcttg ctcacctgcg
     4141 gcagccccca atcgcgcaac atgagcctcg gtcgtgccct aggccagggg caaatcctgc
     4201 agcaggtgct cggcaaccgc cgctcggtgg ccgaaggcgt ctataccgcc gccgccgtgg
     4261 tgcgggcggc ggcgcgagag ggcatcgaga tgcccatttc cgaagccgtg cacgccatcg
     4321 tcgccggcag cattggcacc gatacggcca tcgaggcgct gctgtcgcga ccgcagcggg
     4381 ccgaaagcta gtcgatcggc gggcggcagc gccgcacgcc aggaatcggc ggcgcccagg
     4441 cacattgatc ttggcgctag ttcgcccctt ccagcagccg gcgcgcgatc acctgggcct
     4501 ggatctcggc cgcaccctcg aaaatgttga ggatgcgcgc gtcgcataac aggcggctga
     4561 cgggaaattc cagggcaaag ccattgcccc cgtgaatttg caccgcgttg tcggcgttgg
     4621 cccaggcgat gcgcgcggcc aatagcttcg ccattccggc ctccagatca cagcgccggc
     4681 cgcggtcctt ttcgcgggca gcatggtagg tgagctggcg cgcaatcatc gtttcgatgg
     4741 ccatcatcac gattttgttg gcgacacgcg ggaaggcaaa gatggccttg ccgaattgct
     4801 ggcgcccaag cgcataagcg agggccaggt ccatcgccga ctgggcgacg ccgatcgccc
     4861 gcgcggccgt ctgaatgcgc gccgcctcga aagtcgtcat aagttgcttg aagccctgcc
     4921 cctcgacgcc gccgagcagc tgtgccgtgg gcacctcgaa agcatcgaaa gagatgtcga
     4981 attctttcat gcctcgataa ccgagcacct cgatctcgcc gccggacatg cctgccgccg
     5041 gaaagggatc ggcatcatcc ccgcgcggtt tctcggcaag cagcatggaa aggcctttgt
     5101 agccgggcag gcgcggatcc gtgcgcacga gcaaggtcat cagatcggcc ctgacggggt
     5161 gggtgatcca ggttttctgc cccgtcacct tgtagacgtc gccctcgcgg accgcgcggg
     5221 ttttcagcgc agcaagatcc gagccggtgt tgggctccgt gaacaccgcc gtcggcagca
     5281 atgcaccgga ggcaattctc ggcaggtatt tttgtttttg cgcctcggtc ccggcaccta
     5341 gaatgagctc cgcggcgatt tccgatcgcg ttccaagcga gccgacgcca atgtagccgc
     5401 gcgagagctc ctcggacaca acgcacatgg cttctttgcc gaggccgagc ccaccgaact
     5461 gttcgggtaa cgtcagggca aagacgccga gttcacccag cttgctcacc acctcgctcg
     5521 ggatgtaggc gttcttgagg tgccactggt gcgcgtgcgg caacacctcc gcctcggcga
     5581 agcggcgcat ttgtgagcgc atttccgtca gggtttcgtc gagtcccgtg tcaccgaagg
     5641 tggtgaggcc gggctgctcg gcgatgagcg cggcgaggcg cgccctgacg gcacggtcgg
     5701 tgccttggtc gatcaggccg gcgacgtcgt cttcgaagcg gcgagcctcc gcacgcgcca
     5761 cgccgagctc gctcaggcgc acggtctcat tctggctcat cgggatgccg ccggcgattt
     5821 gcgccaggta ttcgccaaag gcgccggtga gcagaagacg ctccgcttcg ccgaaccggt
     5881 gcgtagccag gagatggcgg ccccagccca gcatctggcg caaggcggcg acgtaggtgg
     5941 caagccaggc aagcccatgc aaggccgcct gtgccgcctc ggcttcttgg gcttccgcgg
     6001 ccgccgcctt tacgccggct ttggccaagt gaaagacgcg atccgccgca ttgagcccat
     6061 cgccgcaggc ctcgagcaaa cgcgcatctt ggcccggacc ggccggcaca atggtcgtca
     6121 cgatcgcttc ctcgcggact tgctgcgcac acgcaccgca tcgcaccata gcggcgccga
     6181 tcgtgtgcgc aagggcgggc gccgacgccg acgttcagcc ctgattggtc tcgccgcgca
     6241 gcgcccgcag cagccgctgg accgcaccgg cgtggttgag ggcgtcgacg ctgacaacgg
     6301 aaacctccgc ttcctcgtcc cgcagcgctt ggctctcggg cagcgccgac cagccaccct
     6361 gcccgtcgac gccaacccag cgctgcgtgg aggaagctgt cgcggacggc tgccagcggg
     6421 cgggcgtcgg cgtgcgcgca acaaaagata cgcttgcctc tgcctcggcc ggcagcaccc
     6481 tcgagggcac gtttggcgcc cccccgctcg tgctggccgc ccctggcgcg ggcgcaaccg
     6541 atgccggccg cgcggtggcg tcgggacggc cgggcgcggt cgtttcgccg ccgggcaggg
     6601 tgcgctcggg ggcgtggcct gggatctcct gcgcggcggg ggcggcgcct gccgaccccg
     6661 cgccgtcgcg ttggcgcgcc tcgatcgccg cgttgaggtt tttccacgcc cggtagatcg
     6721 ggctcgattg caatcgaagt tcgaggctcc ccgcctcctg atcgcgaccg ggcgtagcgg
     6781 cgcctaagcg ctgcaaggtc tgccagctct catcggccgc caacgcaacc tcaaggcgct
     6841 cgcgcgcccg ccgcagtgcg acgagcttgc tctcctcatc aacggacatc acagcttccc
     6901 ttctgccgct cggcgcgacc ggtgcttggg tatgctagcg catttggctg ctgccgtgga
     6961 gccgggcacg ccacctctag gccgaggccg cacggccaac tattctcgcc ggcggctgag
     7021 cgcacaccgc acgccggccg ctcggctcga cggccgcgcc gccaacgcct agggtcgcgc
     7081 cgtcttgcga gccgcctcgc gcactgcggc tagcccctgc aaggacccag gagcaggcat
     7141 gttttctcgc catttgaaac cggttgccat cgccttccac gagacgtttg ccgacagcgg
     7201 cttttccatg gctggggccg tggcctactc gttcgtgctg tcgttgtttc ccttttgcat
     7261 ctttttggcg gcgctggcgg ggtatttcgg gggcgaagcc ttggccgaag ccgcggtcca
     7321 gcagctcttc caactggcac cggcgcccgt ggtcgaggcc atcgcccccg aagtcatgcg
     7381 cgtcatgggc cagagccgtt tcggcctgtt gaccgtcggg gccctgattg cccttttttt
     7441 cgctaccagt gcggtcgaaa gcctgcgcgc ggccctcaac atcgcctatc gcgtcaagga
     7501 gaagcgctcc tacctgcggt gcatggcgca aagcgcactg ttcgtcgttc tcagcgcggt
     7561 gggcatgctc gcgctgacct ggggcgtggt ggtcgggccg caagtggcgg ccggcctaaa
     7621 gccgagttgg ctgctttggc ttaccgatca gggttggacc gctttcatcg ggcgctatgg
     7681 gatcgtcatc atcgctatcg gcggccaact gctggcctat cacctctggc ttgcggccgg
     7741 ctcgcgcggc ctgggcgaag tcttgcctgg tgtcttgctc agcctgctcc tatggattgc
     7801 cgcagcgcgc ctgttcgggt cctggctcag catcagcgac tattcccgct tttatgccgg
     7861 cctcacccag atcatgagcg cgctcgtgtt ctttcaactc tcagccgtcg tcgtcatcct
     7921 cggtgcggag ctgaaccgag gcattctgga aatgcgccgc cgcttagcag agtagcccgg
     7981 cgggtcgtgc gccggctcgc ggtctcaggc ggccggcttg cgcgcgatga tgaccgcctc
     8041 gtccagcgta aagaacaggg cgccgccctc gctgcgcggc ttgagaaagg ccttcaacgc
     8101 cggggaggcc tccagcagaa tggagcgcaa gcgctcaacc gtcggggcgc tgcaacccat
     8161 ccgggttgcc cagggctcga actccatttc tttgggcgcg cgctccttgt ggatgacggc
     8221 caagcccgca tcctcgatca attcgcccca ttccgtcatg ccgaggcagc ggctgtggct
     8281 gggatcacgc agcctttcga acgtattgta gatgagggcg gcatcccgca gctcgatcgg
     8341 tacgaagcct ggcgttgatt cggcgtccgg ctcgatgttg tcggcaagcg caaacgtgcc
     8401 gcctggcttc aggacacgct cgacctcggc tacgaagctc ggaatatcgg gaaagtgatg
     8461 gggggcgatg cggcaggtga cgagatcaaa gcgtgcgtct tcgaagggca acgcttcggc
     8521 gtcggccagc gccgtggaga ggttggcgat gccctttatc tcagccagct ttgccgcttc
     8581 atcgagcatt tgttgcgtca gatcgctggc gatcacgctc gccacatgcg gagcaaaggc
     8641 gagcgcggtg tggccggcgc cggtcgccac gtcaagcgcc tgccactgtt tctgtggctg
     8701 caccagctcc acgagccgcg ctaggctcgc gcctttggcg tgcaccgtcg aggtgacata
     8761 ggcggccgca tgggcgccga attgctcctg cacgagcgat ttgttcatcc tgtctcctcc
     8821 ggccgccgca ggctaacaca ccggcaagcg cgccgcccat ccaccttagc aggcgtggtt
     8881 tcgcaccgcc gctcgagggc tggctcgcca atttaacggc gggcgcaggc cggtgcacct
     8941 gggccggcgc caaaagcttg ggcgacacca tatggctcga gcccgccccc agcgcggggc
     9001 tgtaacggcg gggttgatcg acccgcgggt caggactggg tcggagcagg gagccggccc
     9061 accggctgtc tgccgatggc cgcccgcgcg ccccgcactg ctcggctcac cccaatagcg
     9121 cgtaggtgcc cgttgcaaag gcggcgggct tgtcggtacc ggccgcgctc accgcgcagc
     9181 tgcaaaatgc cagcgtcttg ccgagccgca tcagcttggc ctccaatagc gcgtctgcct
     9241 gactgatggg ccgcatgtag ctgatgctca gatctatggt cgtacagggc ttgaaggcgc
     9301 cgttggcggc cgcaagcgcc aggaccatgg cactatcggc gccgctgatg agtgcctggc
     9361 cgcacagagt tccgcccgtg cggcaaagct cggcggcaaa cggcaggcgc aagacgacgc
     9421 cgtccgcgac gattgtctcc accttgaggc cgctcgccag cacccatggc gcgaagttgt
     9481 ccgcgaggag cgcgttggcc gcctcgcgcg tcaggcccga catggcgagg ttccctttct
     9541 cggaggcggc agctgcacgg cggcggtgga tcgcctgccg ggggcggtcg catcggcaag
     9601 gaccgggata ggataatgcc gccgcgcggg tctgccgttc ggccgggtcg cgatcatttg
     9661 ctacgtgccc gagcgcccgc gccgggccca tcggccccca tcggcaccac gacataggtg
     9721 atctcgacgc tgcccgcttt ttcgaacacc agcgtgccct tgatcgtctg gccggcctgc
     9781 aatggctgtt ttaggtccat catcatcaga tggtgtgcga agggtttgag gaccacgctt
     9841 gcgcccggt
//
LOCUS       DASTMT010000338        17960 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_2481493, whole genome shotgun sequence.
ACCESSION   DASTMT010000338 DASTMT010000000
VERSION     DASTMT010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 17960)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17960)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17960
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2481493"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            67..1284
                     /locus_tag="VFR00_07895"
     CDS             67..1284
                     /locus_tag="VFR00_07895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008594092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HEU0159220.1"
                     /translation="MRRGRDSLSLLEARRVALAAQGFGTERPTPAPDKRGLRRLIERL
                     GAVQIDSVNVLTRAHTLPPFSRLGAYRREDLQALAYARKGRALFEYWGHEASLLPVGL
                     QPLLRWRMARARRGEGLYKALAAFAHERQELIAQVRREVTDRGPLRASELSHQHKGEG
                     GWWGWSDGKRALEWLFWVGELSTATRLETFERAYDLSERVLPRAIVEAPTPAEADAQR
                     QLVKIAAAALGIGSERCLRDYFRLPVADVKLRIAELVEAGELIPLSVEPGFASAYLYR
                     EARLPRRLAAQALLAPFDPLIWQRHRAEVLFGARIRLELYTPREKRMHGYYVLPFLLA
                     DRIVARVDLKADRPRCVLRVQGAHAEPDAPASIIEPLLAELRLLARWLNLERVEVGAA
                     GDLARPLARALRA"
     gene            complement(1303..1953)
                     /locus_tag="VFR00_07900"
     CDS             complement(1303..1953)
                     /locus_tag="VFR00_07900"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU0159221.1"
                     /translation="MSDAIRVAVVDPYPIFREAVVQTITRSEDLRLVAEGATLADARA
                     ALREAQPDILLIDIGLPEGGIEAAVGIAKACAQCKLVVLTALDDAISVSKALAAGVKG
                     YILKGVSGMELVAAIKTIHRGLPFVTPELASRLLTDAKGGALLPMREAKLQAALSYRE
                     QQILSHVSKGLTNKEIADQLGLTVGTIKHYVTQLFKKMNVRNRLEAIQVAQKSEWM"
     gene            complement(1946..3358)
                     /locus_tag="VFR00_07905"
     CDS             complement(1946..3358)
                     /locus_tag="VFR00_07905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0159222.1"
                     /translation="MAAKSLPRLALPLLLPIRGWWQGLERARKLTVALSVGVVCGVLY
                     VCSFVANHIEQAFGHKAAASTALYMDSFVEPLVQELAVKPSLSADNRQALERLLSPAS
                     IGKPVVAFRIWVGDRVVFSNRSDMVGRRFPTSARRERAFQGDVVANFELDGEEEAQER
                     ALHTHILEVYAPVRQTGTNRIIALAEISELAVDLVREIRAAQYASYVVIASAAIGLLL
                     VLFSLTDGLQMRIGELALQRDAHEQFRRRVLRANGRVLEMNERNLRRVGRELHAGPLQ
                     LAALALLKLDALSEPPSPRMDTAATRAADIDVIRKALKECLRQIRDVSAGLAPSEIES
                     LSLAEAISTAVCLYELRTGAAVDCDFRELPQEAAYPLKACLYQLVEEGLAAATRHRTA
                     GSVHVSAVGGETLKIDIAYATEFARASAWLTDDLELKSERVRHQIEALGGTLSLQSPA
                     DRQVRISASFGLGDMGNTNV"
     gene            complement(3565..4458)
                     /locus_tag="VFR00_07910"
     CDS             complement(3565..4458)
                     /locus_tag="VFR00_07910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0159223.1"
                     /translation="MKGELEAGEAEGAQQLPRARKRLPLSAFALGLSASLLLGAASIR
                     LLASWPALAAADRPPAAVGAALAQSPARLDMAIAVGDRPASFPLLVTGLEGLANPRVV
                     LRNLPEALWFSRGERRDEHTWDLAGSDLDDLTVTLRPGTPQAFTLDIEVVAADAVPLA
                     QSVAAVRLIHAASQAAADPSPAPVALRSAAPAEGVVAGAAAPDMAAFKWRTSISASQA
                     VAREARARFAASVSEQPRPAASDPAWRTATLPSPPTERPAGMSALGAVSHEPAAEGRW
                     LWWRLPALAWATARDGESARR"
     gene            4819..6447
                     /locus_tag="VFR00_07915"
     CDS             4819..6447
                     /locus_tag="VFR00_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008596758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HEU0159224.1"
                     /translation="MSGNPYAQHLDKCAANFQPLSPLPFLERAAAIYPQRIAVIHGAS
                     QQTYAQFYARTRRLASALSRRGIGRGDTVCVLLANTPPMLEAHYGVPMLGAVLNAMNT
                     RLDAAVLAFQLDHAEAKVVIYDREFAPVMRAALASAKVRPLVVGYDDPQFPQTQERLA
                     DLDYEALIGEGDAAFAWRLPDDEWDAIALNYTSGTTGNPKGVVYSHRGAALMCYANTL
                     ATGMVRHPVYLWTLPMFHCNGWCFPWTLSLVAGTHVCLRWVRAKAMFAAIAEHRVTHL
                     CGAPIVMSTLLNAAPSEKRQLPHQVEFVTAAAPPPEAVLAAMAEAGFNVTHVYGLTEV
                     YGPAVVNEWQPHWSEFDAGKRAQLKARQGVRYSALEDLTVMNPETMLPVPADGSTLGE
                     VMFRGNIVMKGYLKNEAATEQAFAGGWFHSGDLGVLHPDGYIQLKDRSKDIIISGGEN
                     ISSIEVEEILYKHPAVAACAVVGKAHEKWGETPVAFVELKAGLTASEAEIMAHCRAGL
                     AGYKCPSRIIFAEIPKTSTGKIQKFMLREMAKAV"
     gene            6619..6909
                     /locus_tag="VFR00_07920"
     CDS             6619..6909
                     /locus_tag="VFR00_07920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0159225.1"
                     /translation="MKSRYFSAAVLAAATLWAGTAGAAPIGAGTRAPQAEPILSKVAT
                     FVCARDDRGWHYMRGSRRVTCRPVRPRGPHWGWHCEGPRCGWWHRHERRFND"
     gene            7303..8694
                     /gene="rimO"
                     /locus_tag="VFR00_07925"
     CDS             7303..8694
                     /gene="rimO"
                     /locus_tag="VFR00_07925"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011926698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HEU0159226.1"
                     /translation="MARVAHEKSGRSGVRARPTPRRNTPGRSAPRVGLVSLGCPKAMV
                     DSERIITRLRAEGYQLAADYAGADVVLVNTCGFLNSAKQESLDAIGEALAKNGRVVVT
                     GCFGVEKDRILATHPGVLAVTGPHQYEQVVEAVHAAVPPLHDPFLDLVPPEGLHLTPR
                     HYAYLKISEGCNNRCSFCIIPSLRGRLASRPAADVMREAERLVQAGVKELLVISQDTS
                     AYGLDLKYAESRWRGQALAARFLDLARALGSLGAWVRLHYVYPYPHVDHVLPLMAEGQ
                     ILPYLDIPFQHASPRVLKAMRRPAHQEKTLDRLARWRRLCPDLALRSTFIVGFPGETE
                     EDFALLLDWLKEARLARVGCFKYEAVAGAAANDLPGAVAEELKEERWHRLMSLQQEIS
                     HSLSAARVGQTLAVIIDEVDAEGAVGRSQWDAPEIDGSVYLNGASGLAVGDIVEARII
                     ASDAYDLWGEVGR"
     gene            8805..9752
                     /locus_tag="VFR00_07930"
     CDS             8805..9752
                     /locus_tag="VFR00_07930"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEU0159227.1"
                     /translation="MPQIRLRLRSQPSTGNDPVAGVIWITLAMALFAGLAMFSRMAMN
                     AGLHPFEVVFLRNTFACLMLLPLLLYRGPRLFYSDQFNLYGLRVVISLLSMQTWFYAI
                     FLIPIGEVTAISFLAPLFGTLGAVFLLGEVVRIRRWTALGVGFLGALVILRPVGSEIG
                     LGQVLAIFSAMAAGLTVVLVKQLTAHDDPDKIVFLTNVLMTPLSLVPALFVWSWPTMA
                     VLPALVGMGVCAVLGHICVVRGYAATDASLGMTFEFSKLPFTVAMTYLAFRESIDLWT
                     WVGALIIFASALYITRREAKLARERAQKMQAREATGLGG"
     gene            complement(9773..10726)
                     /locus_tag="VFR00_07935"
     CDS             complement(9773..10726)
                     /locus_tag="VFR00_07935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018273131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HEU0159228.1"
                     /translation="MRTFLLSSLPLLLAMSAAFGAQAVNKTIVVLGTATPGGGFPVYG
                     QAVAESINATDASLEVRPQNTAGSAENIPLLEAGKLDIGLVQGEAAMEALNGVGRPRA
                     DLRVIFAMYATPGMFVVRADSPYHRIEDLRGKRIALGAKGSGLVLLAGYVLDGLGYER
                     SRDFETVLLDRAGDGPEMVANGTVAALWGGGSGWPGFLAVANAPPGARFLVPDAEGIK
                     RIEAKHPFLGTLTVPAGSFPGQTTPIVSVGSWSLILSRPTLADDVAYRLARALHTSEP
                     QLAARLPQARETTAANTARAVRHDLLHPGALKYLKEVGLVR"
     gene            10848..11441
                     /locus_tag="VFR00_07940"
     CDS             10848..11441
                     /locus_tag="VFR00_07940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0159229.1"
                     /translation="MRVRRFFGSVALLLLTALPLEPFEARPPAAAAAELKERTERLAA
                     LFARLTSTNDAVEGDTIVEEIWRVWLDSGRPEIDAALQKALALVETGQPQAAMPLLDS
                     IVERAPEWAEGWNKRATVLYLLDEYDRSLADIDKVLALEPRHFGALAGRGLIHIAQEN
                     YRAALDDYRRARAANPFLQGAAQMIPTLERRVGERPL"
     gene            complement(11464..12240)
                     /locus_tag="VFR00_07945"
     CDS             complement(11464..12240)
                     /locus_tag="VFR00_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007606107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEU0159230.1"
                     /translation="MQLQVSPITREECSAQEWQARIDLAAAHRAAVMHGLHEGIFNHL
                     TLTVPGKPDRYYQIPFGLHWSEVKASSFMEVGLRDGKVKRGVGEVERSCYCIHAPIHD
                     NVAHARAVFHTHMPYASALTRLEDPRIKEIGQTEVLLLDAIAYDDSYMGPAVEPEEGQ
                     RLARIFGDKTILFMANHGITTVGETVAEAYDRLYYVERAAQVQIFAMWTRQPLKQLPQ
                     PVIDKTRTGGGNPSYDPSPSERHFAAIKRMLDRREPDYKD"
     gene            complement(12360..13250)
                     /locus_tag="VFR00_07950"
     CDS             complement(12360..13250)
                     /locus_tag="VFR00_07950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007613906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEU0159231.1"
                     /translation="MSVPNKLASDIVGLISPPRLSRRGFMSASAAATAGYTLAAGPVR
                     AEAITTDTAGLAAGDAKVKVADGDMPVYFAKPANSTNPPVILIAMEIFGLHQYIKDVA
                     RRLAKLGAFAVAPDYYFRKGDLTKISDMKELMPLVNAKPDAELLSDLDASVAWASAQG
                     GDTRRLGIIGFCRGGRTVWVYCANSPKVSAGVAFYGSLVDPSGQKAIWPKSPTELATD
                     MKAPVLGLYGQADQGIPVAQVEAMRAALAAAGKPAEFKIYPEAPHGFHADYRGSYRKE
                     AAEDAWGQMTAWFKTHKVLS"
     gene            complement(13402..14436)
                     /locus_tag="VFR00_07955"
     CDS             complement(13402..14436)
                     /locus_tag="VFR00_07955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008839205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU0159232.1"
                     /translation="MMKKRIALVGLGMAVGPHAKSLVDLADRVEVVTAISPSRQRRET
                     FAERFRFPLGDSLEPVLADPAIDAAAVLTPPNTHLEIVTRLARAGKHILLEKPLEVSS
                     ARAADLVKVCTAAGVSLGVVLQHRFKPAAERLAAVVGAGGLGQIVNASTSIRLWRPQS
                     YYDEPGRGSLARDGGGVLLTQGIHTLDLLLSLVGPAVEVCGYATTSSLHRMETEDLVS
                     AAVHFQSGAFGIIEATTAAYPGGPERIELIGSTGTASLAGTALSIAYHDGRREALAAD
                     ASAGGTGADPMAFPHDYHLGVWRDFLAALDERRPPRVSGREALRVHLLIDALLAAGRS
                     DRKVKVRSSA"
     gene            complement(14433..16232)
                     /locus_tag="VFR00_07960"
     CDS             complement(14433..16232)
                     /locus_tag="VFR00_07960"
                     /inference="COORDINATES: protein motif:HMM:NF017775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HEU0159233.1"
                     /translation="MVDPFLTRMRDARGFASKLWTLTRPYWWAEERQQISVFGWSVVV
                     SEAWIARGLLALIVGLSFLIVYLSKLINAWNARFFNALQEKDAQAFWAELIYWVVLVA
                     LFIVAFVYRQWLTQMLTVRWRRWLSDVYFRDWLADRTYYHMELTRHGTDNPEQRIEQD
                     CASFTTQTLSIALGLLLQVLTLITFASVLWELSGNFVIPLFGGIVIPGYMMWVAVAYA
                     LLGSYATYLIGRPLVAISFELERRNADFRYRMVRIRENAESIALYRGEPDEERSLRSS
                     FASIYDTWWDFMTYNKRLTWITAFYGQAAQVFPLVVAAPQYFAGQITLGVLTQTADAF
                     GQVQGALSWFVDTFAALAGWKAVVDRLTTFSEAMVKAKEVAKRNALERRVAAASELVV
                     EDVEVRLPDGTVLLADVDVTIRQGESVVLRGPSGSGKTTLFRVLAGLWPFGQGRIGVP
                     KGARVLFLPQKPYLPVGTLKDVLSYPDTPEQHADQACCEVLQACGLAALAPRLLEQAN
                     WSLMLSGGEQQRLAFARALLYRPNWLFLDEATSALDDAAEAQMYGLVRTRLADAAVIS
                     VAHRPAVAAMHQRHLVIDAASRRLLRAPAEARP"
     gene            complement(16595..17626)
                     /locus_tag="VFR00_07965"
     CDS             complement(16595..17626)
                     /locus_tag="VFR00_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006206249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HEU0159234.1"
                     /translation="MTAFAHQAAQLDTAGLARYLEERLPGFKGPVEAEKTPTGQSNPT
                     FVLATPSGQYVLRKKPPGQLLKSAHAVDREYRVMKALQDTDVPVPRMLLLCEDDSVLG
                     TAFFVMEHVNGTVFWDPSLPELKGAQRGQLYDEQNRALAALHLVDPARVGLADFGKAG
                     SYYARQRDRWTKQYRASETEHLEDMETLIKWLEANEPPDDGRACLVHGDYRIDNMIFK
                     PDGSQLLAVIDWELSTIGHPFSDLAYQCMQWRFPNHETMRGLANIDRRALGIPTEEDY
                     IAKYCARVGISGIPNWNFYLAFSFFRLAAIVQGVKKRGLEGNASNPEKALKSGERVPL
                     MAKMGVAVI"
     gene            complement(17817..>17960)
                     /locus_tag="VFR00_07970"
     CDS             complement(17817..>17960)
                     /locus_tag="VFR00_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013893077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEU0159235.1"
                     /translation="AMGITQDTPLAHMWTHLRTLRLADGPDAVHRRQVARAELRRYTN
                     QKI"
BASE COUNT         3040 a         5952 c         5847 g         3121 t
ORIGIN      
        1 gccaaggcgc ggtcgcactg gctcgcaccc gtccgccagc tgcggcctcc gcctgggagg
       61 gttatgatgc gacggggacg cgatagcctc tcgctgctgg aggcgcggcg cgtcgccctg
      121 gccgcccaag ggtttggcac agagcgcccg acgccggctc cggacaagcg cggcttgcgg
      181 cgcctaatcg aacggctcgg cgccgttcag atcgattcgg tcaatgtgct gacgcgcgcc
      241 cacaccctgc cgccgttctc gcggcttggt gcctatcgcc gcgaggattt gcaggcctta
      301 gcgtacgcca gaaaggggcg cgctttgttc gagtactggg ggcacgaggc gtccctgctg
      361 cccgtagggc tgcagcccct actgcgctgg cgcatggcgc gggcgcgtcg gggcgaagga
      421 ctctacaagg cgctcgccgc ctttgcgcac gagcgccaag agctgatcgc gcaggtcaga
      481 cgcgaggtta ccgatcgcgg ccccctcagg gccagcgaac tttcccacca acacaaaggc
      541 gaaggtggct ggtggggatg gtcggacggc aaacgcgctc tcgaatggct gttttgggtc
      601 ggtgagcttt cgacggcgac acgcctcgag accttcgagc gcgcctatga tctgagcgag
      661 cgcgtgctgc cgcgagccat cgtggaggcg ccaacgccgg cggaggccga cgcccagcgc
      721 cagctcgtga agatcgccgc cgccgcgctc ggtatcggca gcgagcgctg tttgcgcgac
      781 tacttccgct tgccggtcgc cgacgtcaaa ctgcgcatcg ccgagctggt ggaggccggc
      841 gagctcattc ccctctcggt cgagcccggt ttcgcctccg cctatcttta ccgcgaggcg
      901 cgcctcccgc ggcgccttgc ggcgcaagcc ctactggcgc cgttcgatcc cttgatctgg
      961 cagcggcacc gggccgaggt tctgttcggc gcgcgtattc gcctcgagct ctatacgccg
     1021 cgcgagaagc gtatgcacgg ctactacgtg ctgccgttcc tcctcgcaga ccgcattgtg
     1081 gcgcgcgtcg acctcaaagc ggaccgacct cgctgcgtct tgcgggtgca aggggcccat
     1141 gccgagccgg atgcgccggc ctccatcatc gagccgttgc tggccgagct gcgcctcttg
     1201 gcacgctggc tcaatctaga gcgcgtggaa gtgggggctg cgggcgattt ggctcggcca
     1261 ttggcgcgcg ccctgcgcgc ctgaaggtac ccggcggcag gctcacatcc attccgactt
     1321 ttgggcgact tggatcgcct cgagccggtt gcgcacgttc atctttttaa agagctgcgt
     1381 cacgtaatgc ttgatggtgc ccacggtcag acccagctgg tcggcgatct ccttattggt
     1441 caggcctttg gagacatggc tgaggatctg ctgctcgcgg tagctaaggg ctgcctgcag
     1501 cttcgcctcg cgcatcggca ggagcgcccc gcctttggcg tccgtcagca gtcgggaggc
     1561 caactccggc gtgacgaagg gcagaccgcg atgaatggtc ttgatagcgg caacgagctc
     1621 catgccgctc acgcctttga ggatgtaacc cttgacgccg gcagccaagg ccttggatac
     1681 gctgatggcg tcgtcgagcg ccgtgaggac gacaagcttg cactgcgcgc aagccttggc
     1741 gatgccgacc gccgcttcga tgccgccttc aggcaggcca atgtcgatca agagaatatc
     1801 gggttgcgcc tcgcggagcg ccgcacgcgc gtccgcaagc gtcgcaccct cggccaccaa
     1861 ccggagatcc tcgctgcgcg tgatggtttg caccaccgct tcgcgaaaaa tcgggtaggg
     1921 gtcgaccacg gcgacccgga tggcatcaga catttgtgtt tcccatatcc ccgaggccga
     1981 aactcgccga gatgcggacc tgccggtcgg cgggagactg cagcgacaag gttccgccga
     2041 gcgcctcgat ttggtggcgc acccgctcgc tcttgagttc gagatcgtcg gtcagccacg
     2101 cggaggcgcg cgcgaactcg gttgcgtagg cgatgtcgat cttgagggtc tccccgccga
     2161 cggcgctgac gtgcacgctg ccggcggtcc tgtgccgggt cgcagcggca aggccttctt
     2221 cgaccaattg atagaggcag gccttcaagg gataagctgc ctcctgaggc aactcgcgaa
     2281 agtcgcaatc gacggccgca ccggtgcgca gctcgtaaag gcagaccgcg gtgctgatcg
     2341 cttcggccaa cgacaggctc tcgatttcag aaggggcgag gccggccgag acgtcgcgaa
     2401 tttgtcgcaa gcactccttg agcgctttgc ggatcacgtc gatgtcggct gcacgcgtcg
     2461 cagccgtgtc catgcgcggg ctcgggggtt cggagagcgc gtcgagcttc aggagcgcca
     2521 aggcggcgag ctgcagcggc ccggcgtgca gctcgcgccc gacgcgtcgc aggttgcgtt
     2581 cgttcatctc gaggacccgg ccgttggccc tcaacacgcg ccggcgaaac tgctcatggg
     2641 cgtcgcgttg cagggccagc tcgccgatgc gcatttgcag tccgtccgta agactgaaga
     2701 ggacgagcaa caggccgatg gcagcgctgg ctatcacgac atagctcgca tattgggcgg
     2761 cgcgaatttc ccgaaccaga tcgaccgcca attccgaaat ctcggcgagc gcgataatcc
     2821 ggttggtgcc ggtttgccgg accggcgcgt agacctccag gatgtgcgtg tgcaacgccc
     2881 gctcctgcgc ctcctcttcg ccatcgagct caaagttggc aacgacgtca ccctgaaacg
     2941 cccgctcccg ccgcgcgctg gtcgggaagc gccgacccac catgtcgctg cggttgctga
     3001 agacgacccg atcgccaacc cagatgcgaa aggcgacaac gggcttgccg atcgaggccg
     3061 gagacaacag gcgctcgagc gcctgacggt tgtcggccga gagcgacggc ttgacggcca
     3121 gctcctgcac caagggctcc acgaagctgt ccatgtacaa ggccgtgctg gccgccgcct
     3181 tgtggccgaa tgcctgttcg atgtggtttg cgacaaagga gcaaacgtaa aggacgccgc
     3241 acaccacccc caccgatagg gcgacggtca gcttgcgggc gcgttcgaga ccctgccacc
     3301 agccccgaat gggcaacagg aggggcaggg caaggcgagg cagactcttg gcagccatgc
     3361 gcagcactct atgcctgttc ttgagcctca atacgccgaa aggccggcct tggttatcag
     3421 accaaagtga actcaaagta ttggctcttg gcccggcaac gctttcggcg cccggcatcg
     3481 gccccggtgc ggtcccagtg caacccgagg gcgaagcccc ggagccagcg cgcaccccgg
     3541 ggcggcggac ctcagttgcc accgctagcg acgcgcactc tccccgtccc gagcggttgc
     3601 ccaggccagc gccggcaggc gccaccagag ccagcgtccc tcggccgcgg gctcatgcga
     3661 aacggcgccg agcgcgctca tgccggctgg gcgctcggtt ggcggcgagg gcagggtcgc
     3721 cgtgcgccaa gcggggtcgc tggccgccgg gcgcggttgt tccgacacgg aggcggcaaa
     3781 gcgggcccgc gcctcgcgtg ccaccgcctg tgaggcggag atgcttgtgc gccatttgaa
     3841 ggcagccatg tccggcgcgg ccgcgccagc gacgacgcct tcggctggcg ccgccgacct
     3901 tagcgcaacg ggcgccggcg acgggtcagc ggcggcttgg ctcgcagcat ggatcaggcg
     3961 cacggcggcc acgctttgcg ccaatgggac agcgtcggca gcgacgactt cgatatcgag
     4021 cgtgaaagcc tgcggcgtgc cgggacgcaa ggtcaccgtc agatcatcga gatcgctgcc
     4081 ggccagatcc caggtgtgct catcgcgccg ctcgccgcgc gaaaaccaca gcgcctcggg
     4141 caggtttcgc aagacgacgc gcgggttggc gaggccctca aggccggtca cgagcaaagg
     4201 gaaggacgcg ggtcgatcgc cgacggcgat cgccatgtcc aagcgtgccg gcgactgggc
     4261 tagcgcggcg ccaaccgctg ccggcgggcg atcggcggca gcgagcgcgg gccaactcgc
     4321 cagcaggcgt atcgaggcgg caccgagcag caggctcgcc gacaggccga gggcgaacgc
     4381 cgatagcggc agccgtttcc gcgcccgcgg tagttgttgc gcgccctccg cctcgccggc
     4441 ctcgagctcg cccttcacgg cggcccatcg cgcgattaga ccgttgggcc gccaacgcct
     4501 gacggcagcc ccccgcagct tttcccccat ggcgcccgtc tcccctcgac gtcttgcgcg
     4561 agccccgcca ggcatgctgc ccggccgagc cgaccccccg gtcgcccaaa ttgtccacag
     4621 ttttcccgac gcttgcaaat gtttttgctt gcgcgcccta acttgagcac attcccgcgg
     4681 ggtcgaagcc caccgtcgcg ccatcgcgga cgccagcatc gcgcgccggc agggccgttg
     4741 gaccgcggcg gggaggtcca ctaggttgca gcggctgcat ggccgggacc gcccggacct
     4801 cggcttctgg aggttttcat gtccggcaat ccctatgccc agcacctcga caagtgtgcc
     4861 gccaattttc aaccgctctc gccgctgcca ttcctggagc gcgccgccgc catttatccg
     4921 cagcgcatcg ccgtaatcca cggcgccagc cagcagacct acgcgcagtt ctacgcgcgc
     4981 acgcggcgtc tcgcctcggc cctgtcccgt cgcggcattg ggcgcgggga cacggtgtgt
     5041 gtgctgctcg ccaacacgcc gccaatgctc gaggcccact acggcgtgcc catgctgggc
     5101 gcagtgctca atgccatgaa cacgcggctc gacgccgccg tgctggcctt tcaactcgac
     5161 catgccgagg ccaaggtggt catttacgac cgggaattcg cgccggtgat gcgggccgcc
     5221 ttggcaagcg cgaaggtgcg gccgctcgtg gtcggctatg acgatccgca gtttccgcaa
     5281 acccaagagc gcctggccga tctcgactat gaggcgctga ttggcgaggg cgatgccgcg
     5341 ttcgcttggc gcctgcccga tgacgaatgg gacgcgatcg cgctcaacta cacctccggt
     5401 acgaccggca atcccaaggg cgtggtctat agtcatcgcg gcgcggcgct gatgtgctac
     5461 gccaacacgc tcgcaacggg catggtccgc caccccgtgt atttgtggac cctgccgatg
     5521 tttcactgca acgggtggtg ctttccctgg acgctgtcac tcgtcgccgg cacccacgtg
     5581 tgcctgcgct gggtgcgcgc gaaggcgatg tttgccgcca tcgccgagca tcgggtcacg
     5641 catctgtgcg gcgcaccgat cgtcatgtcg acgctgctca atgcggcgcc gagcgaaaag
     5701 cggcagctgc cgcaccaggt ggaattcgta accgcggcgg cgccgccgcc cgaagcggtg
     5761 ctggccgcca tggccgaagc gggtttcaat gtgacgcatg tctatggcct gaccgaggtc
     5821 tatggccccg ccgtcgtcaa tgagtggcag ccgcactgga gtgagttcga tgcgggcaag
     5881 cgggcccagc tgaaggcgcg tcagggcgtg cgctattccg cgctcgagga tctcaccgtc
     5941 atgaacccgg agacgatgct gcccgtgccc gcggacggca gcactttggg tgaggtgatg
     6001 ttccgcggca acatcgtcat gaagggctac ctcaagaatg aggccgccac cgagcaagcc
     6061 ttcgccggtg gctggtttca ctcgggtgac ctcggcgtgc tgcacccgga tgggtatatt
     6121 cagctcaagg accgctccaa ggacatcatt atctccggtg gggagaacat ttcgtcgatc
     6181 gaggtggagg agatccttta caagcatccc gccgtggcgg cctgcgccgt cgtcggcaag
     6241 gcgcacgaga agtggggaga aaccccggtt gccttcgtcg agttgaaagc gggcctgacg
     6301 gcatcggagg cggaaatcat ggcgcattgt cgcgctgggc ttgccggcta caaatgtcca
     6361 agccgcatca tcttcgcgga aattcccaaa accagcaccg gcaaaatcca gaagttcatg
     6421 ctgcgcgaaa tggccaaggc tgtctgacgc cgcgcgcgaa aaattctggg cgcgcacaac
     6481 tctttgtttg accttcgaga tcacgcctcg ccgcgaaccg ggcgcgcgac ccgcgtctcc
     6541 gcgcgccgga acgaacgcga cggggcggcg tttccaggcg attgcaacgg gacacagcaa
     6601 caaaaatcgg agctcagcat gaagtcacgt tatttcagcg ccgccgtgct cgcggccgcg
     6661 acgctgtggg caggaacggc gggcgccgcg ccgatcggcg ccggcacgcg cgcgcctcaa
     6721 gccgagccca tcctcagcaa ggtcgccacc ttcgtgtgcg cgcgcgacga ccgcggctgg
     6781 cactatatgc gcggcagccg gcgcgtcacc tgtcggcccg tgcgtccacg cgggccgcat
     6841 tggggctggc attgtgaagg ccctcgctgc ggttggtggc atcgccacga gcgccgcttt
     6901 aacgactaaa cttgcaaccc aggcgtgccc agcgctcggg gacctgggct tagtccgggt
     6961 cccctggcgg cttatcggcg tcgcagttgg ctcgcgcgag cctgcgccgc caccgctcgc
     7021 ttggcgccaa gcgccgaccg ccgagatcga gaccgaagcg ggccattctg ccgcgataaa
     7081 actcggcgcg tcccgctcgc gggaaccggc agcgcaggta ttggcttgac gtgaggaaag
     7141 cgctggtcgt ggcacgcgct gcgcagctga ggatcccact tccacgcggg ccttgcgcct
     7201 tggcctcggc gagcgccccc ttgcggcccc ccagcgcggc gcgcccgccg cgcccaaagg
     7261 gagatttgcc ttggctgcaa gcgggcgcta caagcgcgca ccatggcacg agttgcccat
     7321 gaaaaatcgg gccggtcggg cgtgcgcgcc agaccgacgc cgcgtcggaa caccccgggc
     7381 cgctccgcgc cccgggttgg cctcgtttcg ctcggctgcc caaaggccat ggtggacagc
     7441 gagcgcatca tcacgcgcct gcgcgccgaa ggctaccagc ttgcggccga ctatgccggc
     7501 gccgatgtgg tcctcgtcaa tacctgcggc tttctgaact cggccaagca ggaaagcctt
     7561 gatgctatcg gcgaagcctt ggccaaaaac gggcgcgtcg tcgtgactgg ctgcttcggg
     7621 gtggaaaagg atcgcattct cgccacccat cccggcgtct tagccgtgac gggtccgcac
     7681 cagtacgaac aagtcgtgga ggccgtacac gcggcggtgc cgccgctgca cgatcccttc
     7741 ctggatctcg ttcctccgga aggattgcat ctgaccccgc ggcactacgc ctatctgaaa
     7801 atttccgaag gctgcaacaa ccggtgctcg ttctgtatta ttccgagcct gcgcgggcgg
     7861 cttgcaagcc gccccgccgc cgacgtgatg cgcgaggccg aacgactggt gcaggcgggg
     7921 gtcaaggagt tgctcgtcat cagccaggat acctccgcct acggcctcga cctcaaatac
     7981 gcggagagcc gttggcgggg acaagcgttg gcggcacgct tcctcgacct tgcgcgcgcg
     8041 ctcggcagcc tcggcgcctg ggtgcgcttg cactacgtct acccttatcc gcacgtggat
     8101 cacgtgttgc ccctgatggc cgagggccag atccttccct acctcgacat tcccttccag
     8161 cacgcatcgc caagggtgct gaaggccatg cgtcggcccg cgcatcagga gaagacgctc
     8221 gatcgcctcg cccgttggcg ccgcctctgt cccgatctgg cgctgcgctc caccttcatc
     8281 gtcggcttcc ccggcgagac cgaggaggac tttgccctgc tgctcgactg gctcaaggag
     8341 gcccgattgg cgcgcgtcgg ttgcttcaaa tatgaggcgg tcgccggggc cgcggccaac
     8401 gatctacccg gcgcggttgc cgaggagctc aaggaggagc gctggcatcg cttgatgtcg
     8461 ctgcagcagg aaataagtca ctcgctgagc gcggcccgcg tcggccagac ccttgccgtc
     8521 atcatcgacg aggtcgacgc tgagggcgcc gtcggtcgct cgcaatggga tgcaccggaa
     8581 atcgacggat ccgtttatct caacggcgcc agtggtcttg ccgtcggaga tatcgtcgaa
     8641 gcgcgcatca tcgccagcga tgcctacgac ctctggggcg aggtgggcag atgaccagca
     8701 ggccggccgc aaccagttga accgccggct agcctctgcc ccgacatgct tgcaaggccg
     8761 gggcggccag aagcgggcgc ggcgatgcgc gggggatggg tttgatgccg caaatcaggc
     8821 tgaggctgcg cagtcagcca agcacgggca atgatcccgt tgccggggtg atctggatca
     8881 cgctcgccat ggcgctgttc gccgggctcg ccatgttttc gcggatggcg atgaatgccg
     8941 gcttgcaccc cttcgaggtc gtctttcttc gcaacacctt cgcctgtctc atgctgctcc
     9001 ctttgctgct ctaccgtggt ccgcggctgt tctattccga tcagttcaac ctctatggct
     9061 tgcgcgtcgt catttcgctg ctgtcaatgc agacctggtt ttatgcgatc ttcctcattc
     9121 cgatcggtga ggtgaccgcg atcagttttc ttgcgccctt gttcggcacg ctgggcgccg
     9181 tttttttgct gggcgaggtc gtgcgcattc gccgctggac cgcgctcggc gtcggttttc
     9241 tcggtgccct cgtcatcctg cgcccggtcg gctccgagat cgggctgggt caggtgctcg
     9301 ccatcttttc ggccatggct gcgggactga ccgtcgtact cgtcaagcag ctgaccgccc
     9361 atgacgatcc ggacaagatc gtgttcttga ccaatgtgct aatgacgccg ctgtctctcg
     9421 tgcccgcgct ttttgtctgg agctggccga ccatggcggt gcttccagcc ctcgtgggga
     9481 tgggagtatg cgcggtgctt ggtcatattt gtgtggtgcg cggctatgcg gctacggatg
     9541 cttctctcgg catgactttc gagttttcca agctaccttt caccgtcgcc atgacctatc
     9601 tggccttccg ggaaagcatc gatctttgga cgtgggtcgg cgcgctcatc atttttgcct
     9661 ccgcgctcta tatcacccgc cgtgaggcca agcttgcccg cgagcgcgcc caaaaaatgc
     9721 aagcgcggga agccaccggg ctcggcggct gacgcgactt ctacaacttc gctcagcgaa
     9781 ccaatccgac ctccttgaga tatttgagcg cgcccggatg caggaggtcg tggcgtacgg
     9841 cgcgcgcggt attcgccgcc gtcgtctcgc gcgcctgtgg caggcgggcc gccaattgcg
     9901 gctcgctggt gtgaagcgca cgcgccagcc gataggcgac atcatcggcg agcgtcggcc
     9961 gggacaaaat caggctccaa gacccgaccg acacgatcgg cgtggtctgc cccgggaacg
    10021 agccggcggg cacggtcagg gtgccgagga acgggtgctt ggcctcgatt cgcttgatac
    10081 cttcggcatc gggcacgaga aagcgcgccc cgggcggagc gttggccacg gcgaggaagc
    10141 cgggccaacc gctgccacca ccccacaagg ccgcgaccgt gccgttcgcc accatctcag
    10201 gtccgtcgcc ggcgcgatcg agcagaaccg tctcgaagtc gcggctgcgc tcgtagccca
    10261 gcccatcgag cacataacct gcaagcagaa ccagcccgga gcccttggcg ccaagggcaa
    10321 tgcgcttgcc cctgaggtcc tcgatgcggt gatagggact gtcggcacgc accacgaaca
    10381 tgccgggtgt ggcgtacatg gcaaagatca cgcgcaggtc ggcacgcggc cggccaacgc
    10441 cgttgagcgc ctccatggcg gcctcgccct gcaccaaacc gatatcgagc ttaccggcct
    10501 ccaacagcgg gatgttctcg gcgctgccgg ccgtgttttg tgggcgcacc tcgagtgatg
    10561 catcggttgc gttgatgctc tcggctaccg cctgtccata aaccggaaat ccgccaccgg
    10621 gcgtcgccgt gcccaggacg acgatggtct tgttgacggc ctgagcgccg aaagccgccg
    10681 acatcgccaa gagcaagggt aaactcgaca acaagaatgt tcgcatccgt gcactccttt
    10741 ggccccgggg gcatggcttg ctgcggcatt cccagtatag tggcgcctgg ctcggcagcg
    10801 atcaagaatt ggccgtcgcc gccccaaagc ttcaaggtta tggcgccatg cgcgtacgtc
    10861 gcttcttcgg atccgtggcg ttactgctcc tgacggcatt gccattggag cccttcgagg
    10921 cacgcccgcc cgcggccgcc gccgccgaac tcaaggagcg cactgagcgc cttgcggcgc
    10981 tctttgccag gctcaccagc accaacgatg ccgtcgaggg agacaccatc gtcgaagaga
    11041 tctggcgggt atggctcgac tctggacggc cagagatcga cgccgctctg caaaaggcat
    11101 tggctctggt cgagaccggc caaccgcaag cggccatgcc gctgctcgat agcatcgttg
    11161 agcgcgcccc ggagtgggca gagggttgga acaagcgggc aaccgtgctt tacctgctcg
    11221 atgaatacga ccgatcgctt gccgatatcg acaaggtctt ggcgctcgag ccgcgccact
    11281 ttggcgctct ggccgggcgc gggctcatcc acattgccca ggagaattac cgcgccgcgc
    11341 tcgacgatta ccgccgcgcc cgcgccgcca acccgttctt acaaggcgcc gcccagatga
    11401 tacccacctt ggagcgccgc gtcggcgaga ggccgctgtg agcccctcgc cgcgaatcca
    11461 agctcagtct ttgtagtccg gctcgcgccg gtcgagcatg cgcttgatgg cggcaaagtg
    11521 ccgttccgag ggcgaggggt cgtaactcgg gttgccgccg ccggtccgcg tcttgtcaat
    11581 caccggctgc ggcagctgtt tcaggggctg gcgcgtccac atggcaaaga tttgcacctg
    11641 cgcggcgcgc tcgacgtagt agagccggtc gtaggcctcc gccacggtct cccccaccgt
    11701 ggtaatacca tggttggcca tgaataggat ggtcttgtcg ccgaagatcc gcgccagccg
    11761 ttgaccctcc tccggttcga ccgccggccc catgtagctg tcgtcgtagg cgatggcgtc
    11821 gagcaacaga acctcggttt gtccgatctc cttgatgcgg gggtcctcga gccgcgtcag
    11881 cgcgctagca tagggcatat gcgtatggaa cacggcgcgt gcatgcgcga cgttgtcgtg
    11941 gatcggcgcg tgaatacagt agcaggagcg ctcgacctcg ccgacgccgc gcttcacctt
    12001 gccgtcgcgc agcccaactt ccatgaacga actcgccttt acctctgacc aatgcaggcc
    12061 gaagggaatt tgatagtagc gatcgggctt gcccggcacg gtcagggtca agtggttgaa
    12121 gatgccctcg tgcaacccgt gcatcacggc ggcccgatgg gccgccgcca ggtcgatgcg
    12181 agcctgccat tcctgagcgc tgcattcctc acgcgttatg ggcgatacct gaagctgcat
    12241 gcctgtcctc cttggcgagc ctgccgacaa acccgctcga tgcgtcaact ctagcaactc
    12301 gatgctgggt cgggaagatt gtcggcagac cctttgtcct gggtcgccgc gcccaccttc
    12361 tagctcagca ccttgtgggt cttgaaccag gcggtcattt ggccccaggc atcctcagcc
    12421 gcctccttgc ggtagctgcc tcgatagtcc gcgtggaagc cgtgcggcgc ttcgggatag
    12481 attttgaatt ccgccggctt accggcggcg gcgagggccg cccgcatcgc ctcgacctgg
    12541 gcaactggga taccctggtc cgcctgcccg tagaggccga gcacgggagc cttcatgtcg
    12601 gttgccaact ccgtcgggct cttcggccag atcgccttct ggcccgaggg atcgaccagc
    12661 gaaccataga aagccacgcc cgccgagacc ttgggactat tcgcgcagta gacccatacc
    12721 gtgcgcccgc cgcggcagaa gccgatgatg ccgagccggc gtgtgtcgcc gccttgcgcg
    12781 ctggcccacg ccacgctggc gtcgagatcg ctcagcagct cggcgtccgg tttggcattg
    12841 acgagcggca tcagctcttt catgtcgctg atcttggtaa gatcgccctt gcggaagtag
    12901 tagtcgggcg ccaccgcgaa ggctccgagt ttcgccagcc tgcgggcgac gtccttgata
    12961 tactgatgca acccgaagat ctccatggcg atcagaatga ccggcgggtt ggtgctgttt
    13021 gcgggcttgg caaagtagac cggcatgtcg ccgtcggcga ctttgacctt ggcgtcgccg
    13081 gccgcaaggc cggccgtatc ggtggtgatc gcctcggccc ggaccggacc cgcggccaag
    13141 gtgtagcccg cggtcgcagc ggcgctcgcg ctcatgaagc cgcgccggga caagcgtggg
    13201 gggctgatga ggccgacgat atcggaggca agtttgttgg gaactgacat caaggggctc
    13261 ctgaaacatt ccctgtgggt gaccgcctcc cgacaaggca accgccgcgg ctttgcttgc
    13321 gccgctggtc ggccgacctc gtccgctcct cgcccagcct gaagcttcga tcgcagcgcc
    13381 gcactgcacg aatgcgtgaa gtcaggcgct cgagcgcacc ttgaccttgc gatcgctgcg
    13441 gcccgccgcc aagagcgcat cgatcaacag gtgcacccgc agtgcctccc gtcccgacac
    13501 tcggggcggc cgtcgctcgt cgagcgctgc gaggaagtca cgccacacac cgagatgata
    13561 atcgtgcggg aaggccatgg gatcggcacc cgtgccgccg gcggacgcat ccgcggccaa
    13621 cgcttcgcgg cgtccatcgt ggtaggcaat actgagggcg gtaccggcaa ggctggcggt
    13681 tcccgtgctg ccgatgagct cgatgcgttc gggcccgccg gggtaggcgg cagtggtggc
    13741 ttcgatgatg ccgaaggcgc cgctttgaaa gtgcacggcg gctgagacaa ggtcctccgt
    13801 ttccatgcga tgcaggcttg aggtggtggc gtagccgcac acctcgacgg ccgggcccac
    13861 caggctgagc aacagatcaa gcgtgtgaat gccctgggtc agcaacacgc cgccgccgtc
    13921 gcgggcaagg ctgccccggc ccggctcatc atagtaagac tgcgggcgcc ataggcggat
    13981 cgacgtcgac gcgttgacga tctggccgag gccgccggcg ccgacgacgg cggccagccg
    14041 ctcggccgcc ggcttgaagc gatgttgcag gaccacgccg aggctgacgc cggcggccgt
    14101 gcacacctta acgaggtccg ccgcccgcgc actcgaaacc tccaacggct tttccagcag
    14161 gatgtgcttg cccgcgcgcg ccagccgggt gacgatctcc agatgcgtat tgggcggcgt
    14221 cagcacggcg gcggcgtcga tcgcaggatc tgccagaacc ggttcgagac tatcgccgag
    14281 gggaaagcga aagcgctcgg cgaaggtttc gcgccgctgc ctactggggc tgatggcggt
    14341 gaccacctcg accctgtcgg ccaggtccac gaggctcttg gcgtgcgggc caaccgccat
    14401 gccgagaccg accagggcga tacgcttctt catcatgggc gcgcctccgc gggcgcgcgc
    14461 agcagccgcc ggctcgcagc atcgatgaca aggtgtcgct gatgcatggc cgcaacggcc
    14521 ggccggtgcg ccacgctgat cacggccgca tcggccagcc gggtgcgcac caagccgtac
    14581 atttgcgcct cggcggcgtc gtcgagcgcc gacgttgctt catcgagaaa cagccaattc
    14641 gggcgataga gcagcgcccg ggcaaaggcc aagcgctgct gttcgccgcc ggagagcatc
    14701 agcgaccagt tggcctgttc taagagccga ggcgccaggg cggccaaccc gcaggcttgc
    14761 aacacctcgc aacaggcctg gtcggcgtgc tgttcggggg tgtccggata gctcagcaca
    14821 tccttgagcg tgccgacggg caagtagggc ttttgcggca agaacagaac gcgcgcaccc
    14881 tttggcacgc caatgcggcc ttgcccaaac ggccacaggc ccgccaggac gcgaaagagc
    14941 gtggtttttc cgctgccgga tggtccacgc agcaccacgc tctcgccttg gcggatcgtc
    15001 acgtcgacgt cggcgagcaa caccgtgcca tcgggcagcc tgacctcgac gtcttcgacc
    15061 acgagctcgg acgcggccgc gacacgcctc tccaaggcgt tccgcttggc gacttccttg
    15121 gctttgacca tggcttcgct gaaggtggtg agacggtcga ccacggcctt ccagcccgcc
    15181 agggctgcga aggtgtcgac aaaccaagac agcgcccctt gcacctgccc gaaggcgtcc
    15241 gccgtctgcg tcagcactcc cagcgtgatc tggccggcga agtactgtgg tgcggcaacc
    15301 accaagggaa agacctgagc cgcctggccg tagaaggcgg tgatccaggt gaggcgcttg
    15361 ttgtaggtca taaaatccca ccaggtatcg tagatgctgg caaaagagct gcgaagagag
    15421 cgctcctcgt cgggctcgcc gcgataaagg gcaatgcttt ccgcgttttc gcggatgcgc
    15481 accatgcgat agcgaaagtc ggcgttgcgg cgctccagct cgaagctaat cgccaccaag
    15541 gggcggccga tcagataggt ggcataggag ccgagcagcg cgtaggcaac cgccacccac
    15601 atcatgtagc cgggaatgac aatgccgccg aacagcggaa tgacaaagtt gccggacagt
    15661 tcccacagca cactggcaaa cgtgatcaag gtcaagacct gcaacagcag gccgagcgcg
    15721 atgctgagcg tctgggtggt gaacgaggcg cagtcctgct cgatgcgctg ctcggggttg
    15781 tcggtgccgt gccgcgtcaa ttccatgtgg taataggtgc ggtctgccag ccaatcgcga
    15841 aagtagacgt cgctcaacca tcgccgccaa cgcacggtga gcatctgcgt caaccactgc
    15901 cgatacacga atgccacgat gaacagggcg accaacacca cccagtagat cagctcggcc
    15961 cagaacgcct gcgcatcctt ttcctgcagc gcattgaaga agcgcgcgtt ccaagcgttg
    16021 atcaatttcg acaaatagac gatcaagaag ctcagcccca cgatcagcgc caacaggccg
    16081 cgcgctatcc acgcctcgct cactacgacg ctccagccga acacgctgat ctgctgacgc
    16141 tcctcggccc accaatatgg tcgcgtcaag gtccacaact tgctcgcaaa gccgcgcgca
    16201 tcacgcatgc gggtcaagaa gggatcgacc atcggtgcac cagggatgtt gctgaaggag
    16261 gggaagcttg aggattgctc gtcactctac ccccactctt gggcggacaa acgcacaaga
    16321 cagtgatgcc gcgcgcagac ccgcccttgc gcgcgcaccc cggcaaagtg caaatgcgga
    16381 aacgacagcg acgggcgtgc aaagcccgag caccgtgcga gcggtggcct gccgacgagc
    16441 ttcgtatcgc aactgagagc agccgccggg cgggatggct tgggctttcg tgagcgcgga
    16501 cgcagccgtg gacagacttg ccaggcctgc gccggcaaag gcagtattgc ggcgaaggtc
    16561 tcggccttgg gctggtgccg cccgacggca aagctcagat caccgcgacg cccatctttg
    16621 ccatcagcgg cacgcgctcg cccgacttta aggccttctc cggattcgag gcattgcctt
    16681 ccagtcctcg cttcttgacg ccctgcacga tggcggccag gcggaagaag gaaaaggcaa
    16741 ggtagaaatt ccaattcggt atgccggaaa ttccgacgcg cgcgcagtac ttggcgatgt
    16801 agtcctcttc ggtcgggatg ccgagcgcgc gccgatcgat attggccaga ccgcgcatgg
    16861 tttcgtggtt aggaaaacgc cattgcatgc attggtaggc aaggtcagaa aacggatggc
    16921 cgatggtgga aagctcccag tcgatgacgg ctaggagctg cgagccgtcc ggcttgaaaa
    16981 tcatattgtc gatgcggtag tcgccgtgca ccaggcaggc gcgcccatcg tccggtggct
    17041 cgttcgcctc gagccatttg atcagtgtct ccatgtcctc caaatgctcg gtttcggagg
    17101 cgcggtattg cttggtccag cggtcacgct ggcgcgcgta atagctgcca gccttgccaa
    17161 agtcggcaag gccgacccgg gcgggatcga ccaaatgcag cgccgccagc gccctgttct
    17221 gctcatcgta gagctggccg cgctgcgctc ctttgagctc gggcagcgag ggatcccaga
    17281 acacagtgcc gtttacgtgc tccatgacga aaaacgcggt gcccagcacg ctgtcgtcct
    17341 cgcacaacag cagcatgcgc ggtacgggta cgtcggtgtc ctgtagcgcc ttcatgacgc
    17401 ggtactcgcg gtccacggca tgcgcgctct tcaacagctg accgggcggc ttcttgcgca
    17461 agacgtattg cccgctcggg gttgcgagca caaaggtcgg gttagattgg cccgtcggcg
    17521 tcttttccgc ttcgaccggc cccttgaagc cgggcagccg ctcctccagg tatcgtgcaa
    17581 gccctgcggt atcgagctgg gcggcttgat gcgcgaaggc tgtcatgtgg cagcctccga
    17641 atggaagcga cggtgcaggc cgcccgtcgc ttggcgcctt cgccgctgac gcgggcaggc
    17701 cttggacagc agggctgcag ccggcccggg ggcaaagggc gctctttctg tcctaccgct
    17761 ccgagagccg tgaccgcgtt ggaacgccag agcgatggcg cgcatgatcg acaacctcaa
    17821 atcttctggt tcgtgtagcg ccgcagctca gcgcgcgcca cctgtcgccg gtggaccgcg
    17881 tcgggaccat cggcgagccg taacgtgcgc aggtgcgtcc acatatgcgc cagcggcgta
    17941 tcctgcgtga tgcccatggc
//
LOCUS       DASTMT010000339         1610 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_3142103, whole genome shotgun sequence.
ACCESSION   DASTMT010000339 DASTMT010000000
VERSION     DASTMT010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 1610)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1610)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1610
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3142103"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            41..529
                     /locus_tag="VFR00_07975"
     CDS             41..529
                     /locus_tag="VFR00_07975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0159236.1"
                     /translation="MTARRLRQGWLGLGVAVAALSLAPALSAAGAFASLSGSWSGSGN
                     IRLDDGRSEALKCRAYYSPKGEENLGLALRCASSSSKIELRAQLTASGSRVAGHWEER
                     TFNASGTVAGHATGNNIKLAISGGGFSGSMAVTTDGRSQVISVATQGVALKGVNVNLR
                     RD"
     gene            complement(534..1172)
                     /locus_tag="VFR00_07980"
     CDS             complement(534..1172)
                     /locus_tag="VFR00_07980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003600734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU0159237.1"
                     /translation="MPLKAAIVPVTPFQQNCTLLWDEESNRGVVIDPGGDLDRIQAGL
                     SQVGLEVEKIVLTHGHIDHAGGAAELKEKLGGVPIEGPHRADQFLLDGLSAQGSAYGF
                     PETRNVTPDRWLSEGDSVSMAGLSFAVMHCPGHSPGSVVFVNPAQRLALVGDVLFRGS
                     IGRTDFPYGDHAALIDGIKTKLLPLGDDIVFICGHGPMSTLGDERRTNPFLA"
     gene            complement(1294..>1610)
                     /locus_tag="VFR00_07985"
     CDS             complement(1294..>1610)
                     /locus_tag="VFR00_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018270515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEU0159238.1"
                     /translation="KRQTQINGCTFKEGQMVLLSFPAANRDPDVFPEADKVIIDRKEN
                     RHAAFGLGIHRCVGSNLARLEMTVAVEEFLKRIPEFSLAGEVKWSEGTVRGPRLLPVR
                     FA"
BASE COUNT          268 a          509 c          549 g          284 t
ORIGIN      
        1 gtagggctcg gcaaacgggc aaatgggatg ggaaccggcg atgacggcac gacggctgcg
       61 gcagggttgg ctcggcctcg gcgtcgccgt ggcggctctc agtctggcgc cggcgctcag
      121 cgctgcgggt gcctttgctt ccctcagcgg ctcctggtcg ggctccggca acattcgcct
      181 cgatgacggc cgctccgagg ccctgaaatg ccgggcctac tacagcccca agggtgagga
      241 aaacctgggc ttggcgctgc gctgtgcgag ttcgtccagc aagatcgaac tgcgcgcgca
      301 gttgaccgcg agcggcagtc gcgtcgccgg gcattgggag gagcgcacct tcaatgcctc
      361 cggtaccgtg gcgggtcatg ccaccggcaa caacatcaag ctcgccatct cagggggtgg
      421 cttttccggc tcgatggcgg tcaccaccga cggccgtagc caggtcatct cggttgccac
      481 ccagggcgtg gcgctcaaag gcgtcaacgt caacttgcgc cgcgactgag gcctcaggcg
      541 aggaagggat tggtgcgccg ctcgtcgccg agcgtgctca tcggtccgtg tccgcagatg
      601 aagacgatat catcgccgag cggcagcaac ttggttttga tgccatcgat cagggccgcg
      661 tggtccccat agggaaaatc cgtgcgcccg atcgaaccgc ggaacagcac gtcgcccacc
      721 agggccaagc gctgtgctgg attgacgaac acgaccgagc ccggcgaatg gccgggacaa
      781 tgcatgacgg cgaaggatag gcccgccatg ctcacgctat cgccttcgct cagccaacga
      841 tcgggcgtga cgttgcgggt ctcgggaaag ccgtaggcgc ttccctgggc gctgaggcca
      901 tcgagcagga actggtccgc ccgatgcggt ccttcgatcg gcacgccgcc cagtttttcc
      961 ttgagctcgg cggcgccgcc ggcatgatcg atatgcccgt gggtcagcac gattttttcg
     1021 acctcgagac ccacttggct caggccggcc tggatgcggt cgagatcgcc gccgggatcg
     1081 atcacgaccc cgcggttgct ttcctcgtcc cacaggagcg tgcagttctg ctggaagggc
     1141 gtgacgggga cgatggcggc tttgagcggc atggacgatg gaggaggagg tgagggggct
     1201 cggtgcctct agctagcgcg cgccggggcg gtcgtccatg cccccctttg gcgcagcgcg
     1261 agcctttgct gcgctcaagc ctcgcaggct ccctcaggca aaccgaaccg gcagcaggcg
     1321 tggcccgcgc acggtgcctt cggaccattt gacctcgccg gccaggctga attcggggat
     1381 ccgtttcagg aactcctcga ccgcaacggt catctccagg cgcgccaggt tcgagccgac
     1441 gcagcgatgg atgcccaggc caaaggcggc atggcggttt tccttgcggt cgatgatgac
     1501 cttgtcggcc tcgggaaaaa cgtcgggatc gcgattggcc gcggggaagg agagcagcac
     1561 catttgccct tccttgaagg tgcaaccgtt aatctgcgtt tgccgcttga
//
LOCUS       DASTMT010000340         2364 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_2481742, whole genome shotgun sequence.
ACCESSION   DASTMT010000340 DASTMT010000000
VERSION     DASTMT010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2364)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2364)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2364
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2481742"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(53..796)
                     /locus_tag="VFR00_07990"
     CDS             complement(53..796)
                     /locus_tag="VFR00_07990"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEU0159239.1"
                     /translation="MFPIRDSVRRTRDPLVVWTIIGLNIAAFLYQLGLSAPELKYFLY
                     QHALVPRRYFSPGWGNMVGLSPTDFSPFLTNTFLHGGWLHIILNMWTLYIFGPALEDR
                     LGSVRFGILYLLAGVAASATHALFNPASAVPALGASGAIAGVIAAYAVRFPYAWVRIL
                     VPILFIPLFFSIPAVAFAGIWFFMQLIQGTSELFSPWQMGGGIAWWAHIGGFIAGWLL
                     LGLLDPAAPAVRGQPGWYNGGPWERSGRR"
     gene            967..1608
                     /locus_tag="VFR00_07995"
     CDS             967..1608
                     /locus_tag="VFR00_07995"
                     /inference="COORDINATES: protein motif:HMM:NF013488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HEU0159240.1"
                     /translation="MEMIYIADPMCSWCYGFQPVISALAERFETSLPLRLMMGGLRAG
                     QQEPMDAADKAYVRQAWTRVGAATGQPFDMSFFAREHFSYDTEPACRAVVSVRRLQPT
                     LALPFMARIQQAFYAENRDMTAADELAGVAEEAGLAKATFAEAFAAPATRNETFRDFL
                     TAQELGIRGFPTLIAGSRDKGYALVTNGYRPLEDLFEPLERWLAAGAPVSASA"
     gene            1622..>2364
                     /locus_tag="VFR00_08000"
     CDS             1622..>2364
                     /locus_tag="VFR00_08000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015667562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0159241.1"
                     /translation="MSGGLAAHQVLAYNSARQSENKMHDDQVARQFGFGGGLVPGVDV
                     YAYMAHPAVARWGLSFLERGTLTARFLQPVYDGELTTVLPREANAGLDIEVTCRDRVC
                     ASGHARLDTIPAPSLADFAAVPAVAERAAADQATLAPGRWLGIRPLQVSAQFAAQYLA
                     DVRETDPIYAAEGLCHPGLLPRLCNWALMHNVVLGPWIHVGSDIAHFAAVRIGDELSV
                     RAKILANYERKGHHFVELDGVIVANAERP"
BASE COUNT          430 a          783 c          791 g          360 t
ORIGIN      
        1 cgcgcctttt agcttgaagc gccgtccgcc tcaagcacgg catcgcccgg gcttagcggc
       61 gtcccgaacg ctcccagggc cccccgttgt accagccggg ctgaccccgc acggctgggg
      121 ccgcaggatc aagcaggccc agcagcagcc agccggcgat aaagccgccg atgtgcgccc
      181 accaggcgat gccgccaccc atttgccacg gcgagaagag ctcgctcgtg ccctggatca
      241 actgcatgaa gaaccagatg ccggcaaagg cgacggcggg gatgctgaag aacaagggga
      301 tgaaaaggat cggcacgaga atgcgcaccc aggcgtaggg gaagcgcacc gcgtaggcgg
      361 caatcacgcc ggcgatggcg ccggaggccc cgagcgccgg caccgcggag gcaggattga
      421 acagcgcatg cgtggcgctc gcggcgacgc cggccaagag atagagaatg ccaaagcgca
      481 ccgatcccaa gcggtcttcc agtgccggac cgaagatata gagcgtccac atgttgagga
      541 tgatgtgcag ccagccccca tgcagaaacg tgttggtcag gaagggcgag aaatcggtgg
      601 gtgacaaacc caccatgttg ccccagccgg gactgaagta gcggcgcggc accagcgcgt
      661 gctgatagag gaaatacttg agctccggcg cactcaagcc gagttgatag aggaatgcgg
      721 caatgttcag ccctatgatg gtccagacga cgagcggatc gcgcgtgcgc ctgacgctgt
      781 ctctaatcgg gaacattgct cgaagatcac cttgtccgtt gccccctgag atgggaacga
      841 cggcaaccgc cggcaagttc ccccgccgga tggcggccac gtggctagga aatgcggctg
      901 ccgggggcta aattgcaggg gctagaggcg aacgcggcag gggaaaggtt gaggcaagag
      961 gcagccatgg agatgatcta catcgccgat cccatgtgct cctggtgcta cggcttccag
     1021 ccggtgatct cggcgctcgc cgaacgcttc gagacctcgc tgccgctccg cctgatgatg
     1081 ggcggcctgc gcgcgggtca gcaggagccg atggacgccg ccgacaaggc ctacgtgcgc
     1141 caagcctgga cgcgcgtggg cgcggccact ggccagccct tcgacatgag cttcttcgcc
     1201 cgcgaacact ttagctacga cacggagcca gcctgccggg ccgtggtgag cgtgcggcgc
     1261 ctgcagccga cgctcgcctt gccgtttatg gcgcgcatcc agcaggcctt ctatgccgag
     1321 aaccgcgaca tgaccgccgc cgacgagctt gccggcgtcg cggaggaggc ggggctggcc
     1381 aaggcgactt tcgccgaggc gttcgctgcg ccggcaacgc gcaacgagac cttccgcgat
     1441 tttttgaccg cccaggagtt gggcatccgc ggctttccga ccctgatcgc cggcagccgc
     1501 gacaagggct acgcgctcgt caccaacggc taccggccgc tcgaggacct gttcgagccg
     1561 ctcgagcgct ggctggccgc cggcgcgccc gtttcggcat cggcgtgaag gaggctgcag
     1621 catgagtggc ggtctcgcgg cccaccaggt cctcgcctac aacagcgctc gccagtccga
     1681 gaacaagatg catgacgatc aggtggcgcg ccaattcggc ttcggcggcg gcctggttcc
     1741 cggcgtcgac gtctatgcct atatggcgca tccggccgtg gcccgctggg gcttgagctt
     1801 tctcgagcgg ggcacgctca ccgcgcgttt cctgcagccg gtctacgacg gggaactcac
     1861 caccgttctc ccgcgcgagg ccaacgccgg cctcgacatc gaagtgacct gccgcgaccg
     1921 cgtgtgcgcc agcggccacg cccggctcga cacgataccg gcgccatcgc tggccgactt
     1981 cgcggccgtc ccggccgtgg cggagcgcgc cgccgccgat caggcaacgc ttgcaccggg
     2041 gcgctggctc ggcatccgac cgctccaggt gagcgcccaa ttcgcagcgc aataccttgc
     2101 cgatgtgcgc gagaccgatc cgatctatgc ggccgagggg ctgtgccacc ctggcctgct
     2161 gccgcggctt tgcaattggg cgctgatgca caacgtcgtg ctcggtccct ggattcacgt
     2221 cggcagcgat attgcgcact tcgcggcggt cagaattggc gatgagttga gcgtccgcgc
     2281 caagatcctc gccaactacg agcgcaaggg ccatcatttc gtggaacttg acggcgtgat
     2341 cgtggccaac gcagaacgac cgat
//
LOCUS       DASTMT010000341         1678 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_848219, whole genome shotgun sequence.
ACCESSION   DASTMT010000341 DASTMT010000000
VERSION     DASTMT010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 1678)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1678)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1678
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_848219"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..479)
                     /locus_tag="VFR00_08005"
     CDS             complement(<1..479)
                     /locus_tag="VFR00_08005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008872123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU0159242.1"
                     /translation="MLLDKPLASPADIQQRVGRRLSRTQGSPMQTECSADLFGFAPVE
                     GRKVVVGFDGGRFAGCFTGHRTTELVGHTVPSLVGQRVFGIALGYEDLIDHDQLRHDQ
                     AANNSRVGCHPTRSFAILSAMKIATFNVNGVNGRLPVLLRWLDEARPDIACLQELKAS
                     "
     gene            595..1575
                     /locus_tag="VFR00_08010"
     CDS             595..1575
                     /locus_tag="VFR00_08010"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEU0159243.1"
                     /translation="MSTAAEGNAKAAPARVLPWRAGLAAAGTSVMFGFVPLAARGLYA
                     DGLSTWSLLCWRYLLALVIIFAGIRVARLRLGAALRQGAWRITLIGVSLGAAQTLCYF
                     QSLRWLDTGIAVLLFYTFPVVTLGVERFCFKQQVALSALLCVIVIFVGAALIAVPGLH
                     NGTIDPRGLAWAVPGPVIYAFYLAANARLMARHPPLIGAGFLYGGLAAAYLGAALWLG
                     ASLPISAPGWLSLAFLALGPGAVAAIAQSYSMPRLGPPTYAIIANCELVTVVLVGAIV
                     LGEQLTPTGAFGGGLILSGILLHGWVRKPPRDRIVKKPRGAAGRPAAGPP"
BASE COUNT          262 a          562 c          518 g          336 t
ORIGIN      
        1 gacgccttca gctcctgcag gcaggcgatg tccggcctgg cttcgtcgag ccagcgcagc
       61 agcactggaa gccggccatt gacgccgttc acattgaagg ttgcgatctt catcgctgat
      121 aagattgcaa aagagcgggt agggtgacaa cctactctgg aattgtttgc tgcttggtcg
      181 tggcgcaact ggtcgtggtc aatcaggtcc tcgtaaccca aggcgatccc gaatacccgc
      241 tgacccacca agctcggcac cgtgtgcccg accagctcgg tcgtccggtg accggtaaaa
      301 cagccggcaa aacggccgcc gtcgaaccct accaccacct tgcgcccttc aaccggtgca
      361 aagccaaaca aatccgcgct acattcggtc tgcatcgggc tcccctgcgt ccgggacaaa
      421 cgccgcccga cacgctgttg gatgtcggcg gggctggcca acggcttgtc gagaagcatc
      481 gaacacattc gagaggatgt ccgtcagatg cctccggctt gagcgactgc tttccacgct
      541 gtgccccaca cgaagccata ggccgcaaca gcatccccgt agacggcgga tgacgtgagc
      601 actgcagcgg agggcaatgc caaggcggca cctgcgcgag tcttgccctg gcgggcggga
      661 ctggcggcgg ccggaaccag cgtcatgttc ggctttgtgc ctttggcagc gcgcgggctc
      721 tatgccgacg gtctgtcgac ctggtcgcta ttgtgctggc gctacctgct ggcgctcgtc
      781 atcatctttg ccggtatccg cgtggccagg ctgcgcctcg gcgctgcgct gcggcaaggg
      841 gcctggcgca tcaccctcat cggcgtcagc ctcggggcgg cgcagaccct ttgctatttc
      901 cagagcttgc gctggctcga caccggtatc gcagtcctgc tgttctatac cttcccggtg
      961 gtgaccctcg gcgtcgagcg gttctgcttc aaacagcagg tggcgctgtc cgcgctgctg
     1021 tgcgtcattg tgatctttgt cggcgcggca ctgatcgcgg ttccggggtt gcacaacggc
     1081 acgatcgatc cgcgcggtct ggcctgggca gttcccggcc cggtcatcta cgccttctac
     1141 ctggcggcca atgcccggtt aatggcgcgt cacccaccgc tgatcggtgc cgggtttctc
     1201 tatggcggtc tcgcggctgc ctatctcggc gccgcattgt ggcttggggc gtcactgccg
     1261 ataagcgcgc cgggctggct ctcgctcgcc tttctggcgc ttggtcccgg cgccgtcgcc
     1321 gcaatagcgc aatcctatag catgccgcgc ctgggtcccc cgacttatgc gatcattgcc
     1381 aattgcgagc tggtcacggt cgtcttggtc ggcgccattg tgctcggcga gcagctgacc
     1441 cccaccggcg ccttcggcgg cgggctaatc ctctccggca tcctgctgca tgggtgggtg
     1501 cgcaagccgc cgcgggatcg aattgtcaaa aaacctcggg gcgcggcggg tcgacccgcc
     1561 gccggcccac cgtgaggccc ggcgacaccg cccaccacct ctcccgcctc gcggcaaaat
     1621 gcgatattgg ccaagcgtta ccctaactgt taatgcctaa agcctgtttt catcggga
//
LOCUS       DASTMT010000342        10929 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_2483558, whole genome shotgun sequence.
ACCESSION   DASTMT010000342 DASTMT010000000
VERSION     DASTMT010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 10929)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10929)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10929
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2483558"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..197
                     /locus_tag="VFR00_08015"
     CDS             <1..197
                     /locus_tag="VFR00_08015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HEU0159244.1"
                     /translation="RLLNGRQWLCEQYSVLDPYALLFYTWGVRRELPVADLQHYRAFK
                     DRMLARPAVARVLADEKVKV"
     gene            277..1869
                     /locus_tag="VFR00_08020"
     CDS             277..1869
                     /locus_tag="VFR00_08020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011925929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0159245.1"
                     /translation="MRFRILRLAGRAALAAGLLLSAVAAVPAARAESTLKVSLHSDLK
                     IIDPIWTTALISTHHGFMIYDTLFALDEKLEVKPQMVDGWSVSEDKLTWTFTLRDGLE
                     WHDGTPVTAEDCVASLKRWAARDGMAQKLMSFAAELTATDAKTFRLQLKEPYGLVLAT
                     LGKPTSNVPFMMPKRVAETDPNTQLTDTTGSGPFIFKKDEWKPGEKAVYLKNGKYKPR
                     PEPASGFAGGKRVKVDRVEWVWIADPQTQINALSNGEIDFIETPPHDLLGLIGADPNV
                     RSLVVAPMGRQYAFRFNALHKPFDNAKIRQAVAYALNQQDFLEATIGNSKYYVLCKSL
                     YPCGSPLETNKGWEDKLESSVGKAKELLQQAGYDGTPIVLMQSTDIASLSNLAPVAKS
                     LLEKAGFKVDLQAMDWQTLVSRRAKKDPPAAGGWSVYLTSWGSVDVLDPVSTNFLNAS
                     CEKATFGWPCDAELERLRDAFARETDAAKQKVLAEAVSQRVAEYPTHVVLGQYLQPAV
                     FRKNIEGVLVATNVVFWNIEKK"
     gene            1898..2617
                     /locus_tag="VFR00_08025"
     CDS             1898..2617
                     /locus_tag="VFR00_08025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008970246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1028 domain-containing protein"
                     /protein_id="HEU0159246.1"
                     /translation="MTWSIVAHDAASGAFAVAVATRAFAVGAHCPFVRSGVGAVSTQS
                     ITNRYLGPAILDLLERGIAPATAIDSALAADGGCHLRQVHAVDRFGRAAAWTGRHCVE
                     WCGHRAGDQFSVAGNMLAGEAVLADTFEAFAAERTLQLPERLIGAMEAGERAGGDRRG
                     RQSAALLLTTSEDFADLNIRVDDHANPLAELGRLFSIWRAQWPQRAAWAPSKANPSGC
                     TDLDLIEAAWKEQGLDLRFRR"
     gene            2742..3908
                     /locus_tag="VFR00_08030"
     CDS             2742..3908
                     /locus_tag="VFR00_08030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019965984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20 aminoacylase family protein"
                     /protein_id="HEU0159247.1"
                     /translation="MPIINRAAALKDEVSLWRRDMHANPEILYEVHRTAGIVADKLKG
                     FGCDEVVPGIGRTGVVGVIKGRAQTSGKVIGMRADMDALSMTEITGLPYASRTPGKMH
                     ACGHDGHTAMLLGAAKYLCETRNFDGTAVVIFQPAEEGGAGGKAMVEDGLMERWQIQE
                     VYGMHNMPGLPVGQFALRSGPLLAASDGIRIEITGKGGHAARPHQCIDPILVGSHVVT
                     QLHSIVSRNLDPLDAGVISICMFHAGEALNVIPQTAVLEGTARSLTPKVRDLIERRIL
                     EVARATAELHGAVATATYERKYPVTNNHAHQSEFAAAVAGQIAGPDKVDSDIDPVMGG
                     EDFSFMLEARPGAFIFIGNGESAGLHHPKYDFNDDVIPSGISYWARLAETAMPA"
     gene            3905..4798
                     /locus_tag="VFR00_08035"
     CDS             3905..4798
                     /locus_tag="VFR00_08035"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEU0159248.1"
                     /translation="MSVERSGARGAEPSALTPAAIVDALDRRAEKFRTPCGDGSMVWR
                     VWGKGEPLLLFHGGSGSWTHWLRNIPELARHYQLWVPDLPGLGQSAMPPEPLVPATSA
                     DVIVAGLEQLLPGARNLRLAGFSFGGHVAGLTAARLRDRIGALTLIGVAALGLRAEPR
                     ERFAKRRPGMTVEEVAAVFHQNLRVLMFANPANIDALAIHLQAENIKRARFRSRPFAT
                     SDGLAKALAHVTAPLQTIWGREDIIARGSLEARLEIMRQHHPELAVRLIDKAGHWVAY
                     EGADRFNAALLELLAAVGRKS"
     gene            4913..5848
                     /locus_tag="VFR00_08040"
     CDS             4913..5848
                     /locus_tag="VFR00_08040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011085362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-carbamoyl-D-amino-acid hydrolase"
                     /protein_id="HEU0159249.1"
                     /translation="MSRLITVGACQMGPIARGDRRPAVLERLIALLRQAAEHRCDLAV
                     FPELALTTFFPRWWMTAQEEIDAFFEPSMPSTETAPLFEAAQRFGIGFYLGFAELERR
                     AGRVQRFNSSILVDKSGRIVGKYRKIHLPGHAEHEPWRPFQHLEKRYFEVGDLGWGVW
                     RTMGANIGMCICNDRRWPETYRVMGLKDVELVLVGYNTPRHNPPAPDHDRFSEFHNQL
                     CLQAGAYANATWVVGVAKCGVEEGCELIGNSSIVAPSGVVVAQALSQGDELLVARCDL
                     DLAKSYKATTFNFAKHRQPQHYQLIVERTGAVAPE"
     gene            5877..7019
                     /locus_tag="VFR00_08045"
     CDS             5877..7019
                     /locus_tag="VFR00_08045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007604638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HEU0159250.1"
                     /translation="MAYDLILKGGHVIDASQNLDQPMDVAFAAGKVAAVGPNLAGDAH
                     TSVRNVAGKYVTPGLIDLHTHVYWGGTSLGIDAEDFCRRSGVTTAIDTGSAGPGNFAG
                     FRKHVIEPSQVRILAYLHVSFAGIFGFSKTIMVGESENIRLMAPREAAEVADANRDVI
                     VGIKVRVGRHSSGDQGAAPLEMALQVAHEVGMPVMAHIDHPPPSYEEVVAMLRPGDVL
                     THAFRPFPNAPCNAQGTIKPVVLEARKRGVLFDIGHGMGSFAFKTARAMLANGFHPDT
                     ISSDVHALCIDGPAFDQVTTMSKFLCLGVPLRDVIKQSTLNAAFALKRPELGSLKVGS
                     VGDASILSVKEGKFDYVDVMGEHLAGERKICAEGVVIAGGWWHPPA"
     gene            complement(7027..10137)
                     /locus_tag="VFR00_08050"
     CDS             complement(7027..10137)
                     /locus_tag="VFR00_08050"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU0159251.1"
                     /translation="MNVAAGSSPSVTVFGERRLLTILFCDLVDSSQLAGRLDPEDLRE
                     VLTFYQRRATEIVETYGGLVARYQGDGILAYFGYPAASEDDAERAVNAPCELSRRINE
                     GSGVQEVLRVRVGVATGIVVVGDLLRSGAADNPSIVGETPNLAARLQAVAEPDTVLIA
                     ASTQRLTRGLFDYRRLELTGIKGYPLPLEAFQVLGPSEVANRFAALRSTLPLVGRDAE
                     LGRLLALWEKAKAGDGAVAFLRGEPGIGKSRLALELLQRLRKEFPIVRRLYCSPYHKD
                     SMLYPLLTQLQRTAQIDVSDSVTAKLEKLQALLVGAGETAGALTAVLADLLALPLPER
                     EKLEPLDARQKRELLFKILVEAFEGLARRRSLIVVLEDIHWIDPTSLELLSTIVARLQ
                     GLPALLVITARPGSEPGWLGRENVHTFNLGPIDQGSAEALAQRICGIADAGNSTLRGI
                     VARADGVPLFIEELSKAALEAGAMGPAAEAGAVAAASSMAIPASLHASLLSRLDRLGS
                     AREVARAAAAFGREFAFDHLAGVMETWSPSDLRAALEQLIEADLIVPIGPPPWTAFAF
                     RHVLIQDAAYSTLLRGERRALHGRIARTLTQLFPDVVAIGPEIVAAHFSKAELFEPAV
                     ALWLDAGVRAADRSAFAEAIQHFSEGVRLSRMLPDDPARARKELDLQMALGPALMATR
                     GYAAPESLEVFARADQLVASVGTVAERLDVLLGLFNVHYGRAELERSLEVARAHLALS
                     EQHGQGASRAHCLLGQTYSAMGAFVDANRHFEQALAMFAQQPEVAGSWGVMASQHVVT
                     LALSAGVHFALGELQLARAAITAAVDRARAIEHPLSIALAIVTDVLTPNPGDVEGSHR
                     RAEEAVEFCAHHGLKNFEAWARFAQGAILTRRGAVAHGIEVMQDAVAAAEALGSRLFR
                     PAQLATLAGAYARLGDCARALALVEEAIGIAQLTGERQALAGIERVKGELLFALGRPG
                     EGEAALNAARQTARAQGALSEQRRVEASLARFLSKPSAPSGRSRAPAAQPARGWLGML
                     RALLGI"
     gene            complement(10457..>10929)
                     /locus_tag="VFR00_08055"
     CDS             complement(10457..>10929)
                     /locus_tag="VFR00_08055"
                     /inference="COORDINATES: protein motif:HMM:NF024934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEU0159252.1"
                     /translation="EPGDTFGEIGPLDGGARTADATALTEVQVMSLQRAKFNALLEGN
                     PRVARAAIKFLCGRLRETSELAEGIALHSIEMRLARFLLSALKRQRSDRGTSQSVIEL
                     GITQGELALLIGASRQKVNAALNTLEQAGAVRRTRTRLFCDEPVLLRVAALEQS"
BASE COUNT         1913 a         3720 c         3521 g         1775 t
ORIGIN      
        1 atcgcctgct caacggccgg cagtggcttt gcgagcaata ctccgtgctc gatccttacg
       61 cgcttctctt ctacacctgg ggcgtgcggc gcgagctgcc ggtggccgac ctccaacact
      121 atcgggcgtt caaggatcgc atgctggcac gccccgccgt tgcgcgcgtg ctggccgatg
      181 aaaaggtcaa ggtttgagtt gatagcggcc gtgcgccgca cgctgcgggg gaaaacggcc
      241 ccgaaggctt ttttcgccta acgaaggatt gctaccatgc gcttcaggat actgcgcctt
      301 gccggccgcg ccgccctggc ggcgggcctt ctgctgagcg ctgtggccgc ggtgcccgcg
      361 gctagggccg agagcacctt gaaggtctcg ctgcactccg acctcaagat catcgatccg
      421 atctggacca cggcgctgat cagcacgcat cacggcttca tgatctacga cacgctgttc
      481 gcgctcgatg aaaagctcga agtcaagcca cagatggtcg acgggtggag cgtcagcgag
      541 gacaagctca cctggacctt taccctgcgc gatggcctcg agtggcacga tgggacgccg
      601 gtgacggcgg aggattgcgt ggcctcgctc aagcgctggg cggcgcgcga cggcatggcg
      661 caaaagctca tgagcttcgc ggccgaactg acggcgaccg acgccaaaac cttccgcctg
      721 cagttgaagg agccctacgg cctggtgctg gcgaccctgg gcaagcccac gtccaacgtg
      781 ccgttcatga tgccgaaacg cgtggccgag acggacccca acacgcagct caccgatacc
      841 accggctcgg ggcccttcat cttcaaaaag gacgagtgga agccgggcga aaaggccgtc
      901 tatctcaaga acggcaaata caagccccgc cccgagccgg cctccggctt tgccggcggc
      961 aagcgcgtga aggtcgatcg cgtcgaatgg gtctggatcg ccgatccgca gacgcaaatc
     1021 aatgcgctca gcaacggcga gatcgacttt atcgagaccc cgccgcacga tctgttgggg
     1081 ctgatcggcg ccgatcccaa cgttcgttcc ctggtggtgg cgcccatggg gcggcaatat
     1141 gctttccgtt tcaacgcgct gcacaagccg tttgacaacg ccaaaatccg tcaggccgtg
     1201 gcctatgccc tgaaccagca ggattttctg gaggccacga tcggcaattc caaatactac
     1261 gtcctgtgca agtcccttta cccctgtggc tccccgctcg agacgaacaa gggctgggaa
     1321 gacaaactcg agagcagtgt tggcaaagcc aaggagctgt tgcagcaggc cggctacgac
     1381 ggtacgccga tcgtgctcat gcaatcgacc gacatcgcct ccctttccaa tctggcgccg
     1441 gtggccaagt cgctgctcga gaaagccggc ttcaaggtcg acctgcaggc gatggactgg
     1501 caaaccctgg tctcccggcg cgccaaaaag gatcccccgg ctgccggcgg ctggagcgtc
     1561 tatctcacct cctggggctc cgtcgacgtg ctcgatcccg tctcgaccaa cttcctcaac
     1621 gccagctgcg agaaggcaac cttcggctgg ccctgcgatg ccgagctgga gcgcctgcgc
     1681 gatgccttcg ccagggagac cgacgcggcc aagcaaaagg tgctggccga ggcggtctcg
     1741 cagcgcgtgg ccgaatatcc cacccacgtg gtgttgggac agtacctgca gcccgccgtc
     1801 tttcgcaaaa atatcgaggg cgtgctggtg gcgaccaacg tggtgttctg gaacatcgaa
     1861 aagaaatagg cgcccgaggc gccacggctt tgccagcatg acctggtcga ttgtcgctca
     1921 cgatgctgcc agcggcgctt tcgccgtcgc cgtggccacg cgcgcctttg cggtcggtgc
     1981 gcactgcccc ttcgtgcgct ccggggtggg cgccgtttca acccaatcga tcaccaatcg
     2041 ctatttgggt ccggccattc tcgacttgct cgagcgcggc attgcccctg cgacggccat
     2101 cgacagcgcg ctcgccgccg acggggggtg ccacctgcgt caggtgcatg cggtcgaccg
     2161 ctttgggcgg gccgcagcat ggaccggcag gcactgcgtg gagtggtgcg ggcaccgcgc
     2221 cggcgaccag ttttccgtcg ccggcaatat gctggccggc gaagccgtgc tggccgacac
     2281 cttcgaggcc tttgcggccg agcgcacact tcagctgccc gagcgcctca tcggcgccat
     2341 ggaggccggg gaacgggccg gcggcgaccg acgcggccgc cagtcggcgg cactcctatt
     2401 gacgaccagc gaagacttcg ccgacctcaa cattcgggtc gacgatcacg ccaacccgct
     2461 tgccgagctg ggccgcctct tcagcatctg gcgcgcgcag tggccgcaac gcgccgcctg
     2521 ggcgcccagc aaggccaacc cctccggctg tacggacctc gacctcatcg aggctgcctg
     2581 gaaagagcaa ggtctcgact tgcgctttcg ccgctgagcg caatgctgac ctgtgcacgc
     2641 ctcggcctgc cttgacccgt ggaaggcgat ttgcttccct gccgcgcgcg ccacgcgccg
     2701 cgccgagtta gctgccgttc aggatagccg caggaattaa gatgcccatt atcaaccgtg
     2761 ccgccgctct caaggacgaa gtctcgcttt ggcgtcgtga catgcatgcc aaccccgaga
     2821 ttttgtacga ggtgcaccgc actgccggta tcgtcgccga caaactcaag ggattcggct
     2881 gcgacgaggt cgtacccggc atcggccgca ccggcgtggt cggcgtcatc aagggccgcg
     2941 cccagacctc tggcaaggtg atcggcatgc gcgccgacat ggatgcccta tcgatgacgg
     3001 aaatcaccgg cctcccctat gcctcacgca cgcccggcaa aatgcacgcc tgcggccacg
     3061 acggacacac cgccatgctg ctgggtgccg ccaaatatct gtgcgagacg cgcaatttcg
     3121 acggcaccgc cgtcgtgatc ttccagccgg cggaggaagg cggcgccggt ggcaaggcca
     3181 tggtcgagga tgggctgatg gagcgctggc agatccaaga ggtctacggc atgcacaaca
     3241 tgccgggcct gcccgtcggc cagtttgccc tgcgcagcgg tcccctgctg gcggcaagcg
     3301 atggcatccg tatcgagatc accggcaagg gcggacatgc ggcgcgcccg catcagtgca
     3361 tagatccgat cctggtcgga tcgcacgtcg ttacccagct ccattccatt gtatcgcgca
     3421 acctcgatcc gctcgatgcc ggcgtaattt ccatttgcat gttccacgcc ggcgaggcgc
     3481 tgaacgtcat cccgcagacc gcggtcctcg agggcaccgc ccgctcgcta acaccaaagg
     3541 tgcgcgacct aatcgagcgg cgcatcctcg aagtggcgcg ggcgaccgcg gaactgcacg
     3601 gcgccgtcgc cacggcaacc tatgagcgca aatatcccgt caccaacaac catgcgcacc
     3661 aatcggagtt tgccgccgcc gtggcgggac aaattgccgg acccgataag gtcgatagcg
     3721 acatcgaccc ggtgatgggt ggggaggatt tttcctttat gctggaggct cgccccggcg
     3781 ccttcatctt cattggcaac ggcgagagcg ccggcctgca tcaccccaaa tacgacttca
     3841 atgatgacgt gatcccatcc ggtatctcct attgggcacg cctggccgaa acggcgatgc
     3901 ccgcgtgagc gtcgagcgat caggcgcgag gggcgccgag cccagtgcgc ttacgccagc
     3961 ggcaatcgtc gatgcgctgg accgccgcgc cgaaaaattc cggacgccct gcggcgatgg
     4021 cagcatggtg tggcgcgtct ggggcaaggg tgagccgctc ctgctgttcc acggcggctc
     4081 cggctcgtgg acgcattggc tgcgcaatat tcccgagctt gcgcggcact accagctctg
     4141 ggttccggat cttcccgggc ttggccagtc ggcgatgcca cccgagccgc tcgttcccgc
     4201 aaccagcgcc gacgtcatcg ttgcggggct cgagcagctg ttgccaggcg cgcgtaacct
     4261 gcggttggcg ggcttctcct tcgggggcca cgtcgccggc ctgaccgcag cgagactgcg
     4321 cgaccgcatt ggcgcgctca ccttgatcgg cgtggccgca ctcggcttgc gcgccgagcc
     4381 ccgcgagcgc ttcgccaaac ggcggcccgg catgaccgtc gaggaggtcg cggccgtgtt
     4441 tcatcagaac ttgcgggtgc tgatgtttgc caaccccgcc aacatcgacg cgctcgccat
     4501 ccatctgcaa gccgagaaca tcaagcgggc gcgcttccgc agccgcccgt tcgccaccag
     4561 cgacgggctc gccaaagcgc tggcccacgt cacggcaccg ctgcaaacga tctgggggcg
     4621 cgaggacatc atcgcgcgcg gctcgctcga ggcccggctc gaaatcatgc gccagcatca
     4681 tcccgagctc gcggtccggc tgatcgacaa ggccggccac tgggtggcct acgagggcgc
     4741 tgaccgcttc aacgccgccc tgctggagct gctggcggcc gtcggccgca aaagttaaca
     4801 acccggccag ggccgaaccc ctccgggtta tccgctggcc ctgccacctc agttgggcaa
     4861 tcattggcgg caacgcgccc ggcccgggcc gcgccacggc gttggaggca gcatgtcacg
     4921 gctcatcacc gttggagcct gtcagatggg accgatcgcg cgcggcgatc gccgcccggc
     4981 cgtgctcgag cggctcatcg cgctgctgcg ccaggccgcc gagcaccgct gtgatctcgc
     5041 cgtctttccg gaattggcac tgaccacgtt ctttccgcgc tggtggatga cggcgcagga
     5101 ggaaatcgac gcttttttcg aaccgagcat gccctcaacg gagaccgcgc ccctgttcga
     5161 ggccgcgcaa cggtttggca ttggctttta cctggggttc gccgaactcg aacggcgcgc
     5221 gggccgcgtc cagcgcttca acagctcgat cttggtcgac aagtccggcc gaattgtcgg
     5281 caagtaccgc aaaattcatt tgcccgggca tgccgaacac gaaccctggc gcccgttcca
     5341 gcatctggaa aagcgctact tcgaggtcgg cgatctcggc tggggcgtgt ggcgaacgat
     5401 gggcgccaat atcggcatgt gcatttgcaa cgaccggcgt tggccggaaa cctaccgggt
     5461 gatgggcctc aaggacgtgg agctggtgct ggtaggctac aacacccccc gccacaatcc
     5521 gccggcgccc gaccacgacc gtttcagcga gtttcacaac cagctatgcc tgcaggcggg
     5581 ggcctatgcc aacgcgacct gggtggtggg cgtcgccaaa tgcggcgtcg aggagggctg
     5641 tgagctgatc ggcaattcct caatcgtcgc ccctagcggg gtggtcgtgg cgcaggcgtt
     5701 aagtcaaggc gacgagctcc tcgtcgcgcg ctgcgacctc gatttggcca agagttacaa
     5761 ggcgacaacc ttcaacttcg ccaagcaccg ccaaccgcag cactatcagc tgatcgttga
     5821 gcgcaccggt gccgtggcgc ccgaataagc gaacctcgaa cactgtgggg gccaacatgg
     5881 cttacgatct cattctcaag ggcggacatg tcatcgatgc ctcgcaaaac ctcgatcaac
     5941 cgatggacgt ggccttcgcc gcgggcaagg tggccgcggt tggccccaac ctcgcaggcg
     6001 atgcgcacac atccgtgcgc aacgtggcgg gcaagtacgt gacgcctggc ctgatcgacc
     6061 tgcacacgca tgtctattgg ggcggcacct cgcttggcat cgatgcggag gatttctgcc
     6121 ggcgctccgg tgtgacgacc gccatcgaca ccggcagtgc gggccccggg aatttcgcgg
     6181 ggttccgcaa gcacgtgatc gagccgtcgc aggtacgcat tctcgcctat ctgcacgtct
     6241 cgttcgccgg catcttcggc ttttccaaaa ccatcatggt tggcgagagc gaaaatatcc
     6301 gcctgatggc gccgcgcgag gccgccgagg tcgccgacgc caaccgcgac gtgatcgtcg
     6361 gcatcaaggt ccgcgtcggc cggcactcct cgggtgatca gggcgccgcg cccctggaaa
     6421 tggcgctgca ggttgcccat gaagttggga tgccggtgat ggcgcatatc gaccatccgc
     6481 cgccctccta cgaagaggtg gtggcgatgc tgcggccggg cgacgttctc acccatgctt
     6541 ttcgcccgtt tcccaacgcg ccgtgcaacg cccagggcac catcaagccg gtggtcctgg
     6601 aggcgcgcaa acgcggcgtg ctgttcgaca tcgggcatgg catgggctca ttcgcgttca
     6661 agaccgcacg cgccatgctg gcgaacggtt tccatcccga cacgatctca tcggacgtgc
     6721 acgctctgtg catcgacgga ccggctttcg accaggtcac caccatgtcg aagtttctct
     6781 gccttggcgt gcctttgcgc gacgtcatca agcagtcgac gctcaacgcc gcgttcgcct
     6841 tgaagcggcc cgagctcggc tcattgaagg tcggcagcgt cggcgacgcc agcatactct
     6901 cggtcaagga gggcaagttc gactacgtcg atgtcatggg cgagcatctt gccggcgagc
     6961 gcaagatctg cgcggaaggc gtcgtcatcg ccggcggctg gtggcacccc cccgcctgag
     7021 gcagcctcaa attccgagca aggcgcgcaa cataccaagc cagccccggg ccggctgcgc
     7081 ggccggcgcg cggctccgac cggagggcgc cgacggtttg ctcagaaacc gcgccaggct
     7141 cgcctcgaca cggcgctgtt cggagagtgc accttgggcg cgggcggtct ggcgggccgc
     7201 gttgagggcg gcctcgcctt cccccggccg accgagggca aacagcagct cgcccttgac
     7261 gcgctcaatc ccggcaagcg cctggcgctc accggtcagc tgggcaatgc cgatcgcctc
     7321 ctcgaccaga gccaaggcgc gcgcgcaatc gccaagcctg gcataggcac ccgccagcgt
     7381 cgccagctgc gctggccgga acaggcgcga gcccagcgcc tcggccgcgg cgaccgcgtc
     7441 ctgcatcacc tcgatgccgt gcgctacggc gccgcgccgg gtcaagatcg cgccctgagc
     7501 gaagcgcgcc caggcctcga agttcttcaa gccatggtgg gcacaaaact cgacggcttc
     7561 ctcggcacgt cggtggctgc cctccacgtc gcccggattg ggggtcaaca cgtccgtgac
     7621 gatcgccagg gcaatggaca agggatgctc gatggcacgc gcccgatcga cagccgccgt
     7681 gatcgccgcg cgcgccagct ggagctcgcc caaagcaaaa tgcacacccg cactgagcgc
     7741 cagtgtgacc acgtgttgac tggccatcac gccccaggag ccggcgactt ccggttgctg
     7801 ggcgaacatc gccagagcct gctcgaaatg ccggttggca tcgacaaacg cacccatggc
     7861 cgaataggtc tgtcccaaca ggcagtgggc gcggctcgcg ccctgcccat gctgttcgga
     7921 gagcgccaga tgagcgcgcg ccacctccag cgagcgctca agctcggctc tgccatagtg
     7981 cacgttgaag agccccaaca gtacgtcgag ccgttcggcg acggtgccca cactggccac
     8041 caattgatcg gcgcgggcga acacctcgag actttccggg gcggcatagc ctcgcgtggc
     8101 catcagcgcc ggcccaagcg ccatttgcag atccagttcc ttgcgggcgc gcgcagggtc
     8161 gtccggcagc atccgcgaaa gccgcacgcc ctccgagaaa tgctggatcg cttcggcaaa
     8221 ggccgagcga tcggcggcgc gaacacccgc gtccagccac agggccacgg ccggctcgaa
     8281 cagctccgcc ttgctaaagt gggccgccac gatctctggc cctatcgcca cgacgtcggg
     8341 aaagagctgg gtcagcgtcc gggcgatgcg cccgtgcagg gcgcgccgct cgccccgcaa
     8401 caacgtgctg taggcggcat cctggatcag cacgtgccgg aaggcgaagg ccgtccaggg
     8461 cggtggcccg attggcacga tgaggtcggc ttcgatcagc tgctcgaggg cggcgcgcag
     8521 gtcggacggg ctccacgtct ccatgacgcc cgccaggtgg tcgaaggcaa attcgcggcc
     8581 gaaggcggcg gcagccctgg ccacctcgcg tgcggacccc agccggtcga ggcgcgacaa
     8641 aagcgaagca tgcaggctgg cgggaatggc catgctgctc gcagcggcga cggcgccagc
     8701 ttcggcggcg gggcccatgg cgccggcctc gagcgcagcc ttgctcagct cctcgatgaa
     8761 caacggcacc ccatcggcgc gggcgacgat accccgcagc gtgctgttgc cggcgtccgc
     8821 gatgccgcaa atgcgctgcg ccagcgcttc ggcactgccc tggtcgatcg gccccagatt
     8881 gaacgtgtgc acgttttcac gcccgagcca gccgggctcg ctgcccggcc gcgccgtgat
     8941 caccagcagc gcgggcaggc cttgcaggcg cgcaacaatg gtgctgagca gctccagact
     9001 ggtcggatcg atccaatgga tgtcttccag cacgacaatg agcgagcgtc tgcgcgccag
     9061 gccctcaaaa gcctcgacca ggatcttgaa cagcagctcg cgcttttggc gcgcatcgag
     9121 cggctcgagc ttttcccgct cgggcaacgg cagggccaac aaatccgcca gcaccgccgt
     9181 cagcgctccg gccgtttccc cggcccccac caacagcgct tgcagcttct ccagcttggc
     9241 ggtgaccgaa tcgctgacat cgatctgggc cgtgcgctgg agctgggtca gcagcggata
     9301 gagcatgctg tccttgtgat aaggcgaaca gtaaaggcgc cgcacgatgg ggaactcttt
     9361 tctgaggcgc tgcagcaatt ccagcgccag gcgcgacttg ccgatgcccg gctcgccgcg
     9421 caggaaggcg acggcgccgt cgccggcttt ggccttctcc cacagcgcca acaggcgacc
     9481 gagctcggca tcccgcccca ccaacggcaa ggtcgagcgc agggcggcga agcgattggc
     9541 gacctcgctc ggccctaaca cttgaaaggc ctccagcggc agcgggtagc ccttgatgcc
     9601 ggtgagttca agccgtcggt agtcaaacag accacgcgtg agccgttgcg tgctggcggc
     9661 aataagaacc gtgtccggct cggcgacggc ctgcaaccgc gccgcgagat tgggcgtctc
     9721 cccgacgatg gagggattgt cggcggcgcc cgagcgcaag aggtctccga cgacgacgat
     9781 gccggtcgcc accccgacgc ggacgcgcaa gacctcctgc accccgctgc cctcgttgat
     9841 gcgccggctg agctcgcagg gcgcgttcac ggcacgctcg gcgtcatcct cgctggcggc
     9901 cggataaccg aagtaggcga ggataccgtc gccttgatag cgcgccacca ggccgccgta
     9961 ggtctccacg atctcggttg cacggcgctg gtaaaaggtc agcacctcgc gcaggtcctc
    10021 cggatcgagc cgtccggcca gctgcgagga atcgacgaga tcgcagaata ggatggtgag
    10081 cagccggcgc tcgccgaaca cggtcacgga aggggaggac ccggccgcca cgttcatggg
    10141 ctcccaacct cggccggggt ggcctgggtg cgaacagcgg agcccagcac cccgggggtt
    10201 tccccggcgc aagctggctt cgatgctggc ggctccctga tgatgcgcac gaaaaagcct
    10261 atggcggtgg cagagacccc ggcgccctgt cactcaagtg acagacctta gcagcggttg
    10321 ggcgcaacat gaaagcgcat tggtcggcgg cacagggcaa tccgtgccga tttgtccggt
    10381 gcggggctgc ggccttagga ttgcccagca ggcgggccac aggcgcgggg tcctgtggcc
    10441 cgttcgcatc aaaggctcag gactgctcca gggcggccac ccgcagcaac acgggttcgt
    10501 cgcaaaaaag ccgggtacgg gtgcgccgga cggcgccggc ctgctccagt gtgttgagcg
    10561 cggcattgac cttttgccgg ctggcgccaa tcagcaaggc gagttcgccc tgggtaatac
    10621 cgagttcgat cacggactgg ctggttcccc gatcggaacg ctggcgcttg agcgccgaga
    10681 ggagaaaacg cgcgagccgc atctcgatgg aatgcagcgc aatgccttcc gccaactcgc
    10741 tggtctcgcg caggcggccg cataaaaact tgatcgcggc ccgcgccacg cgcgggttac
    10801 cctccaacag ggcattgaac ttggcgcgct gcagcgacat cacctgcacc tcggtcagag
    10861 ccgtggcatc ggcggtccgc gcacccccgt cgagcggccc gatctcgcca aaggtatcgc
    10921 ccggctcgg
//
LOCUS       DASTMT010000343         2593 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_6004759, whole genome shotgun sequence.
ACCESSION   DASTMT010000343 DASTMT010000000
VERSION     DASTMT010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2593)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2593)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2593
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6004759"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2593
                     /locus_tag="VFR00_08060"
     CDS             <1..>2593
                     /locus_tag="VFR00_08060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0159253.1"
                     /translation="IVAFGSRRGGDISTSYAQSLRLIVKNAVLWVMFLSSMAASVFFS
                     FDSHFNAIFPAEARARAAEIRTTSQIGSAIADIGAVAQRRQLEEAERLFETQGWKSYD
                     KQLALLSRQAQDSQGEIEKYFVAQMEERRRAVNQQQERISTAQGGQAGLAQKKVTLTE
                     ELSRLKAERPGLAADYAQHRGELDAKAREIDAKRIEGMAEDRGVEGSMKQGKGPIYRQ
                     RMGELALLNDQYKIKEERSKDAFRRLNGVETRVAQIERELSGIDGDLAKLKGEAQTAE
                     QRIKATLAASSGEEDGKLDPARVLPGFERARAAFHQQPTTERLAFLQQQCSNLLAAMA
                     STEAIKGKLRSVDCDPKQAAEAAGGVFALNAGIAAFQTRCAGGDKLNGLNTTDALLGF
                     GRNCLQESGLIGKDSAESGARLAAIEMNRDDKAHRFVVTWNAFLDGNRLAYLALVLAI
                     GVDSLVFMSGLFGAQALRSPLSDVPSPKARSAEQLQAVIDTALLPHTYENARAMLNAM
                     RPMAPREGFTQRVTLREDDPHAADLNRLLNAGATIGAVRHVEANTYELRSELFEYLSL
                     VAKKSFGADKTHVVLAELERIMAVSLLPNVGENVETVLRYVHPIEDRSRFLDKLGRPG
                     ARGDFTAEIRLDEVLDRGDKKVVRNALNAGATLEAVERANNNHYLISRDFYKALARIR
                     AHLLIGAAPQALQIASAGGGSSGSAQRLRDFSHPALPSGTPGRAAEPRRLLPRLGEAA
                     ANAAKDPELRDKIWLELLDALGLTPASAARLSQDLVREQASAACRALGHLAQTNQRLR
                     GHLKELEGAKRDALRQKEGDLVGRFAGDSWRLAVLAETRRDLEAHLKPLLLLPEAGLV
                     QELIERLE"
BASE COUNT          464 a          871 c          852 g          406 t
ORIGIN      
        1 atcgttgcct tcggctcgcg acgcggcggc gacatttcca cgtcctatgc gcagagccta
       61 cgcttgatcg tcaagaatgc cgtgttgtgg gtgatgttcc tctcctccat ggccgcctcg
      121 gtcttctttt ccttcgactc ccacttcaat gcgatcttcc ccgccgaggc acgcgcgcgc
      181 gctgccgaaa tccgcaccac cagccaaatt ggttcagcca tcgccgatat cggtgccgtg
      241 gcgcagcggc gccagctgga ggaggccgag cggctattcg agacgcaggg ctggaagtcc
      301 tacgacaagc agctcgcact gttgagccgg caggcccaag actcgcaagg cgagatcgag
      361 aagtacttcg tggcgcagat ggaggagcgc cggcgcgccg tcaatcagca gcaggagcgc
      421 atcagcaccg cccagggcgg tcaggccggc ctcgcgcaaa agaaggtcac gctgaccgag
      481 gagctgtcgc gcctcaaggc ggaacggccc ggccttgccg ccgactacgc ccaacaccgc
      541 ggcgagctcg acgccaaggc ccgcgagatc gacgccaaac gcatcgaggg tatggctgag
      601 gaccgcgggg tcgaaggcag catgaagcag ggcaaggggc cgatctatcg ccagcgcatg
      661 ggcgagctcg cgcttctcaa cgaccagtac aaaatcaaag aggagcgcag caaggacgcg
      721 ttcaggcgcc tcaacggcgt ggagacgcgc gttgcccaga tcgagcgcga actctccggc
      781 atcgacggcg atctcgccaa gctcaagggc gaggctcaga ccgccgagca gcgcatcaag
      841 gcgacgctgg cggcgagttc cggggaggaa gacggcaagc tcgatcccgc ccgcgtgctg
      901 ccaggcttcg aacgcgcccg tgccgcattt caccagcaac cgacgacgga gcgcctggcc
      961 tttctgcagc agcaatgcag caatttgctc gccgccatgg cgagcacgga agccattaag
     1021 ggcaagctgc gcagcgtcga ttgcgatccc aaacaggcgg cggaagcggc cggcggcgta
     1081 tttgccctca atgccggcat cgccgccttc cagacccgct gcgctggcgg cgacaagctg
     1141 aacggcttga acaccaccga tgccctgctc ggtttcggcc gcaactgcct gcaggagtcg
     1201 gggctgatag gcaaggattc ggcggagtcc ggcgcccggt tggcggcgat cgaaatgaac
     1261 cgggacgaca aggcgcaccg gttcgtcgtg acctggaacg cttttctcga cggcaatcgg
     1321 cttgcctatc tcgccctcgt tctcgccatc ggcgtcgatt ccttggtgtt catgtcgggc
     1381 ctgttcggcg cccaggcgct ccgctcaccg ctctccgacg ttcccagccc gaaggcgcgc
     1441 agcgccgagc agctgcaggc agtgatcgac acggcccttt taccgcacac ttacgagaac
     1501 gcgcgggcca tgctgaatgc catgcggccg atggccccgc gcgaaggctt cacgcagcgc
     1561 gtcaccctgc gggaggatga tccgcatgcc gccgatctca accgcctgct caacgccggc
     1621 gccaccatcg gggcggtgcg ccacgtcgag gccaacactt acgaattgcg ctccgagctg
     1681 ttcgaatatc tctcgctggt ggccaaaaaa tcgtttggcg ctgacaaaac gcacgtggtt
     1741 ctggccgagc tcgagcgcat tatggcggtg tctcttttgc ccaacgtggg cgagaacgtc
     1801 gagacggtgt tgcgctacgt gcacccgatc gaggatcgct ccaggttcct ggacaaattg
     1861 ggccggcccg gcgcgcgcgg cgatttcacg gccgagatca ggctcgacga ggtcctcgac
     1921 cgcggcgaca aaaaggtggt gcgcaacgcg ctgaatgccg gcgccacgct ggaggccgtg
     1981 gagcgcgcca acaacaacca ctatttgatc agccgcgact tctacaaggc gctggcccgc
     2041 atccgtgccc acctgttgat cggcgcggca ccgcaagcct tgcagatcgc ctcggccggc
     2101 ggcggctcct ccggctcggc gcagcggctg cgggacttct cccacccggc gctgcccagc
     2161 ggaacacccg gtcgcgccgc cgaaccgcgg cggcttctgc cgcggctcgg ggaggccgcg
     2221 gcgaacgcgg ccaaggatcc ggagctgcgc gacaagattt ggctggagct tctggacgcc
     2281 ctcggcctga cgccggcgtc ggcggcgcgc ctcagccaag acctcgtgcg cgagcaggcg
     2341 agcgcggcct gccgcgcgct cggtcacttg gcccagacca atcagcggct gcgtggtcat
     2401 ctcaaggagc tggagggcgc caagcgcgat gcgctgcgcc agaaggaggg cgacctcgtc
     2461 ggtcgttttg ctggcgattc ctggcgcctg gcggtgctgg ccgagaccag gagggatctc
     2521 gaggcccacc tcaagccact tttgctgtta cccgaagccg gcttggtgca ggagctgatc
     2581 gagcgcctgg aag
//
LOCUS       DASTMT010000344        15480 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_5348889, whole genome shotgun sequence.
ACCESSION   DASTMT010000344 DASTMT010000000
VERSION     DASTMT010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 15480)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15480)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15480
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5348889"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            407..1477
                     /locus_tag="VFR00_08065"
     CDS             407..1477
                     /locus_tag="VFR00_08065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018318899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU0159254.1"
                     /translation="MPSRRRMMQLLGAASAAAAGGWAVLAPSRSNAYYRGPVSDHFDG
                     TVFFNPGGVKPKNQLAILKWQLTDRGVAWPDAFASPFQPDRPPPRFAGAGARITHVGH
                     ASFLIQTHGRNILLDPVWAERASPLSFLGPRRVNAPGIAFEALPHIDIVLITHNHYDH
                     MDVATLGALWQRFRPRMVTPLGNDTILRNSIPGLEARALDWDARVDLDGGIGVHVEPT
                     LHWSARGTSDRRHALWASFVLAAGTQRIYCVGDSAYGDGSSFRRVRQRHPRLTLALLP
                     IGAYEPRWIMRNNHMNPEEALEALELCGAERALGHHWGTFRLTNEAVDEPPQRLSAAL
                     ARAGVAAGRFLAARPGQVVLLS"
     gene            complement(1615..2298)
                     /locus_tag="VFR00_08070"
     CDS             complement(1615..2298)
                     /locus_tag="VFR00_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012115237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEU0159255.1"
                     /translation="MTYAFPPIALPTLPIRGSDKLFPIHRIYCVGRNYADHAVEMGHD
                     PNKEPPFFFQKNADNVATDGTFPYPAASTDVHFEIELVVALKSGGVDIRPADAMKHVF
                     GYAVGIDMTRRDLQAEAKRLQRPWEVGKAFEHSAPCSSLVLAQHVENSSQGAIWLEVN
                     GERKQSGNLNQMIWDIPHQIAFLSGLFALKGGDLIFTGTPAGVGATKRGDRLQGHIDG
                     IGDLQVSVI"
     gene            2479..3735
                     /locus_tag="VFR00_08075"
     CDS             2479..3735
                     /locus_tag="VFR00_08075"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018323621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HEU0159256.1"
                     /translation="MFPLSHMLKSFVRKGTLKVIDADGNAHVFAGGPGPSVTMRLGDR
                     TLYRKLFFNPELHAGEAYMDGRLSFEGSTLRDFLTLFSVNRLALGAYPLQNLLRRISR
                     GLKRFQQANPIGKAEKNVAHHYDLGNDFYRLFLDQGMQYSCAYFLSDSDSLEEAQQNK
                     LRLLAAKLNLRPGLRVLDIGSGWGDLALYLARLEDVDVTGVTLSREQYALANEKARAA
                     GLGDRVRFELRDYRQVTEPFDRIVSVGMFEHVGVRHYREFFAKVRALLDPDGVMLLHS
                     IGHMSPPGTASPWLRKYIFPGAYSPALSEVFTAVEQESLWVTDLEFLRLHYAKTLNHW
                     CRRFEANRARIAQMYDERFCRMWEFYLISAEMMFRTGSQLVFHMQLAHKRDAVPIVRD
                     YVTDLQRKYRETEAHMANRPFAKADG"
     gene            4190..4510
                     /locus_tag="VFR00_08080"
     CDS             4190..4510
                     /locus_tag="VFR00_08080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0159257.1"
                     /translation="MPIPSAPATAPITGPATPAPALPALSPMSDPLPTQFATGGGSSP
                     NLALSPATSASSPSETAPSTPGGGGKSLQDCMGFWDAGTHMTKPEWRAACKRSMAEFP
                     DIKW"
     gene            complement(4538..5518)
                     /locus_tag="VFR00_08085"
     CDS             complement(4538..5518)
                     /locus_tag="VFR00_08085"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEU0159258.1"
                     /translation="MAGIPRRTFAQGLAAVAVSAPTRAAAQPGYPAGLTVKFVVGFPA
                     GGVQDIVGRVIADRLGALWKVPTVVENVAGAGGNLGMDRVAKGPSDGSQICIVPPGIA
                     TNPYLYARVPFDPEKDFVLLGHVASVPNLLCVRKDLPVDTVAELIAHAKANPGKLNYA
                     SSGIGTTVHLSAELFKRMTGVDMVHVAYRGSAPALNDLVGQNVDLMFDNITSIIGQAR
                     AGAVKPLGITLTQRSTLAPEFPPIAETVPGYDTTSWTGVAVRAGTPKAICDKIEADTK
                     VICQDAMLKERLAGLVAETVGTGAAEFTAYVGAERAKWGKLITDLRLRVE"
     gene            complement(5637..6479)
                     /locus_tag="VFR00_08090"
     CDS             complement(5637..6479)
                     /locus_tag="VFR00_08090"
                     /inference="COORDINATES: protein motif:HMM:NF016908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HEU0159259.1"
                     /translation="MLEFEMNALPWGTFRPGLTVRALIGLSRHTLLGRGKLRKQLARL
                     LFLLHAGPVDCWLWGHRIRLFPDHNVCERKALLRPDRMDRQEYGFLRTQLARARSVFV
                     DVGANAGLYSLYAALNAGAGARILAIEPDAALLARFTFNLRLARVARYAAPDIEVTTV
                     PVAVGEHDGEALLSTSGDEGSRSLRGSGVGRPVQVRRLAALLADHAVGKITVLKIDVE
                     GYEDRVLPPYLAEVPQEHWPDAIIIEHVYRPSWSVDCIKLCQGHGYRISGITNNNTLL
                     ARGA"
     gene            complement(6540..7598)
                     /locus_tag="VFR00_08095"
     CDS             complement(6540..7598)
                     /locus_tag="VFR00_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015664952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HEU0159260.1"
                     /translation="MSYIAKPSAHHPALPKNASGLTRRDYEGTMSTLCAGCGHDSITA
                     ALIEACFEMDLPAHRIAKVSGIGCSSKAPTYFLSRSHGFNSVHGRMPSITTGAAIANR
                     DLVYIGVSGDGDTASIGLGQFCHAIRRRLNMTYIVMNNGCYGLTKGQFSATNDKTSPS
                     KKGEANPFEAIDLCQLAIQLGAGFVARSFSGDKNQLIPLIKAAIGYKGFAFLDVVSPC
                     VTFNNHPTSTKSFDYVRAHNMASENPLDYVEPRDEITVDYAEGSSAAIALHDGSRLVL
                     HKLDAGHDPRDADAAIMVIRRNDKQGRIATGLLYIDESQQDMHDVLVSTDQPLNQIAM
                     SQLCPGAAALDAINQRLR"
     gene            complement(7607..9427)
                     /locus_tag="VFR00_08100"
     CDS             complement(7607..9427)
                     /locus_tag="VFR00_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012029370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HEU0159261.1"
                     /translation="MSGINDFVVKFATVNGSGSASANTIFAKTLFRMGLAVSPKNIFP
                     SNIQGLPTWYEVRVSDAGYIARRDGIDLMVAMNPQTFKEDLAEVVAGGWLLYDSTLPR
                     VWPRDDISVLGIPIAQLCAHKWSDPRQRQLFKNMVYVGALTALLDLDIDVLKTAIVDQ
                     LKGKEKLVAANMEAVSLGRDFALANFHCPLPVRVRTAACSKGKIMVSGNDAAGLGAVY
                     AGATVCAWYPITPSTSLAEAYERYARRLRVTKEGKRLYGIVQAEDELAAIGIAIGGGW
                     NGARAFTATSGPGISLMSEFLGLAYFAEVPVVLFNVQRGGPSTGMPTRTQQSDIVSCA
                     YASHGDTKQVLLFPANPNECFSMGAQAFDLAERLQTPVIVMSDLDIGMNEWVTEPLVW
                     DDAHVHDRGKVLDAAALEGFKDLKGRDWGRYLDVDGDAIPYRTLPGTHPSKGAFFTRG
                     TSHDENARYTEDGRAHARILDRIRRKFDTAATLVPKPEVSIRDKNAKMGIIYFGANAP
                     AVLEALDLLERDGIKINALRIKAFPFSKEIVAFCEDHDTLVVVEQNRDAQMRSLLMTE
                     IDVPGGKLIRALNYDGMPMTAAFVRGAVLKELRPAQAAAE"
     gene            complement(9447..11252)
                     /locus_tag="VFR00_08105"
     CDS             complement(9447..11252)
                     /locus_tag="VFR00_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015684121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU0159262.1"
                     /translation="MAATNTSDPKYFHKVVDCQWACPTHTPVPEYIRLIAQKRYAAAY
                     MLNWESNVFPGILGRVCDRPCEPACRRGRVEEKPVAICRLKRVAADYKGEIDAFLPDV
                     PREKNGKRIALVGAGPASLTVARDLMPLGYTCILFEKDTRGGGLMRTNIPSFRLPETV
                     LDEEIDRILGFGVEAHFDSEIKSLEALLGQGFDAVFIGTGAPKGKDLEVPGRRQVAAN
                     IHIGIDWLTSVAFEHVSRIGKRVVVIGGGNTAMDCCRTALRLGGEDVKVTVRSPRRVM
                     KASPWEIEDAEHEGIPIFDNHAPKEFVIEHGKLAGVRFQLMREVGIDTRGRPRLEPSG
                     DEVVFACDDVLMAIGQENSFPWIERNIGIEFDDWGMPKVDKATMMSTRAGVFFGGDAA
                     WGPENIIWAVAHGHQAAVSIDLFCRGKSLNERPAPGSSLFSQKMGIHEWSYKNDYAAQ
                     RRVGMPHVDKAQALRNLKVEVELGFDDQLAFREAERCLNCDVQTVFSAPLCIECDACM
                     DICPVDCINFTANAEESSLRGKLRVPARNKTQDLYVSSPLKTGRIMAKDEDVCLHCGL
                     CAERCPTGAWDMQKFMYVEAQAAPACLTHHNAYLR"
     gene            11638..13473
                     /locus_tag="VFR00_08110"
     CDS             11638..13473
                     /locus_tag="VFR00_08110"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC oxidoreductase"
                     /protein_id="HEU0159263.1"
                     /translation="MHRLASGLRLIRSHYDAVVVGSGYGGGVAAARLARAGKRVAVLE
                     RGREIPVGEFPRRFSDLRNDLQLSGRQTQLGSPTGLYDVRLGEDMHVLVGCGLGGGSL
                     INAGVALRPDARVFEDPAWPSELARDPLLEQGYLEARRWLRPARDPGAHERSKLKALG
                     GASVALGVKPVAAPVTVSFAECLNPAGIAQPACTLCGDCCGGCNVGAKNTVALTYLAD
                     AVRHGAEIFTHAKVRDLAPDGGGRWCVHFQVQPGADQAALEAIGAVSADLVVLAAGTL
                     GSTEILLRSRDRGLALSDRLGHRFSANGDIIAFGWGGRLPVGAIGVGHPAKLKDSVVG
                     AAVSGQIEIVDAGDLANSLTIQEGALPSAMAPILPVLFLPNGRLLGALQSLISGVYKG
                     PFARLQTFFAVSHDSAAGQLRLEADRVVLSWPGASAEPVYRRLDSALEALVCGAGGSY
                     VKNPLAGTIMGHHPATAHPLGGCSIGHDHRDGVVNHKGQVFAPGGAGGDVHDGLYVMD
                     GSIVPRSLGANPLLTITALAERAMAHMARDQGWREAAASALNLGSGLGPGLAAGARQT
                     PVRLPALASASHHIQIENGADRARDDEAREETAHVGAPLLGAQRR"
     gene            complement(13360..13848)
                     /locus_tag="VFR00_08115"
     CDS             complement(13360..13848)
                     /locus_tag="VFR00_08115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0159264.1"
                     /translation="MSSLPALAIDNTAPQLPPQPRSMRLSDAEKIAADRLDTTPISSL
                     KAALAGVTAAALPTAPRLVERIDAAAKLAADATAAAEAVETLKRLLQAQLATNRPPAE
                     STAVVPLSVGPRAVPARAAEALPSAPLGAEQRRPDVRGFLAGFVISGAIGAVLYLYMM
                     AG"
     gene            complement(13991..15403)
                     /locus_tag="VFR00_08120"
     CDS             complement(13991..15403)
                     /locus_tag="VFR00_08120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0159265.1"
                     /translation="MISIQSAMLVALGFLAASLLGLLLASAFWGRAVRLTTARIKQSL
                     PVSEQEIQADRDRLRAEYAIKVHKLETHLEQAKLARARQLVELNRRDASISTLETNVG
                     ELKAELEEHQNARHVLEQTIADRLPKVEARLGEAKRLIISREREIAELLENAKRHKLA
                     LEEAASINAQQTSQIERLTSAVAARSARSKQAGDAGGEAEVALRSELEALRAKARDQA
                     NVIDRLQRSASEPQVVASAGVSAVEATPSRSQEVLAQAEAALASARSGAAEPDKGTPL
                     EAELRLLKTKADDQAGEITRLRAALATFEGESGTQGASKESRAALKARLSSAQAQADH
                     QSAMVGRLRAELAAVNERLARQAAHFMDEMRRLGAGTLPVAAQPRPSARADRRPLAER
                     VAQVRSTTPGPRSSAKEAGAPEPEKLEGPPAPVRNGVDVEGPSQRPAVSAAEMPKGGD
                     IPEEPRRLRLLDRITSLAKE"
BASE COUNT         2583 a         5043 c         5034 g         2820 t
ORIGIN      
        1 gcatcatcga tccggccaag gttgtgcgca cggctctgca ggatgcagcc tcgattgccg
       61 gcctgctcat caccaccgag gccggcgttg ccgaggcacc gaagaaaaag gacgcgcacg
      121 ccgggcacgc acacggcggc atgggcggca tggacatgta aggctgccca ccgcctacgt
      181 ctgcaatcgg gccgcccgcg cttcggcatg ggcggccgtt tctttcggcg cacgctcggc
      241 tcacaagctt ggccgctgcg acgggccagg cctctggcag atcaagcctg ttgcaggcgg
      301 ccgcgcccgc gccttgccgc gcgctcggcg ttccatcgcc cttgccccgg cccatatctg
      361 tcactattca aggcacgggg tgcgcaccac ctgatggaag gttggcatgc cgagccgacg
      421 gcggatgatg cagttgctgg gggccgcctc ggcggcggcc gcgggcggtt gggccgtgct
      481 ggcacccagc cgcagcaacg cctattaccg cggccccgtg tcggaccatt tcgatggcac
      541 ggtgttcttc aatccaggcg gtgtcaaacc aaagaaccag ttggccatcc tcaaatggca
      601 gctcaccgat cgaggcgttg cctggccgga tgccttcgca agcccgtttc aacccgatcg
      661 gccaccgccc cgcttcgctg gggcgggggc gcgtattacc cacgtcggtc acgcttcatt
      721 cctgatccag acgcacggcc gcaatattct gcttgatccc gtctgggccg aacgcgccag
      781 cccgctgagc ttcctcggac cgcgccgggt aaatgcgcct ggcatcgcct tcgaggcgct
      841 gccgcacatc gacatcgtcc tcattaccca caatcactac gatcacatgg atgtggcgac
      901 gctgggcgcg ctctggcagc gcttccgtcc tcgcatggtg accccgctcg gcaacgacac
      961 catcttgcgc aacagcatcc ctgggctcga ggcccgagcc ttggactggg acgcgcgcgt
     1021 cgatctggac ggcggcattg gggtgcacgt cgagccgacc ttgcactggt cggcgcgcgg
     1081 caccagcgac cgccggcacg ctctgtgggc aagtttcgtt ttggcagccg gaacgcaacg
     1141 gatctattgc gttggcgaca gcgcttacgg cgacggatca agcttccgcc gcgtgcgtca
     1201 gcgccatcct cgtctgacgc tggcgttgct gccgatcggt gcctacgagc cacgctggat
     1261 catgcgcaac aatcatatga atcccgagga agcgctcgag gcattggaac tgtgtggggc
     1321 ggagcgcgcg ctggggcatc actggggcac ctttcggctc accaacgagg ccgtcgacga
     1381 gccgccgcaa cgcctgtcgg cggcgctggc gcgcgccggg gtcgcggcgg gccgcttcct
     1441 cgccgcccgc cctgggcagg tcgtgctctt gtcgtgacgg tcgagcctgg tgcgataagg
     1501 cacgaaggtg tggggctgcc cggcaccgac ctcggccgta tcgcctgtgc gtgtcccgta
     1561 ccggggctcg cgggcgggct ttgccgacgc gctcctggct ccggcgggtg cgggctagat
     1621 gacgctcacc tgcaagtcgc cgatgccatc gatgtggccc tgcaggcgat caccccgctt
     1681 ggtggcgcca acgccggcgg gcgttccggt gaagatcagg tccccgcctt taagggcgaa
     1741 cagtcccgac agaaaggcga tctggtgcgg aatgtcccag atcatctggt tgagattacc
     1801 gctttgcttg cgctcaccgt tgacctcgag ccagattgcg ccctggctcg agttctccac
     1861 gtgctgggcc aacaccagcg acgagcaggg cgcggaatgc tcgaaggcct tgcctacctc
     1921 ccatggacgc tgcaaccgct tggcttcggc ttgcaaatcg cgccgcgtca tgtcgatgcc
     1981 gacggcataa ccgaacacgt gcttcatggc gtcggccggc ctgatgtcga ccccgccgga
     2041 ttttagcgcc accaccaact cgatctcgaa gtggacgtcc gtgctggccg caggataggg
     2101 aaaggtgccg tcggtcgcga cgttgtcggc gttcttctga aaaaagaagg gcggttcctt
     2161 gttgggatca tgccccattt cgacggcatg gtccgcatag ttgcgcccca cacaataaat
     2221 tcggtggata ggaaagagtt tgtccgagcc acgaatgggc agcgtcggta gggcaatggg
     2281 tgggaaggcg taggtcatga tagcgtaccg ctttcggggc aaggttcgcg ggcgttggta
     2341 gggcatgctg cgtccgcgcg caagcgggtg tccgccacaa actggccgcg gggcaagccc
     2401 caaccggtcc ttggctgcgg cgctgaccgc ggcgcgcccg gcttggctgc tgcgctgcga
     2461 gatgagcgag gaaggagcat gtttccacta tcgcacatgt tgaagtcatt cgtgcgcaag
     2521 ggcaccctta aggtcatcga tgccgatggc aacgcgcacg tctttgccgg cgggcccggc
     2581 ccgagcgtaa ccatgcgcct cggcgatcgc accctttacc gcaagctttt tttcaatccc
     2641 gaactgcatg cgggggaggc ctatatggac ggccgcctca gcttcgaagg ctcaacgctg
     2701 cgcgattttc tcaccctctt ttccgtcaat cggttggcgc tcggtgccta cccgctgcaa
     2761 aaccttctgc gccgcatctc gcgcgggctc aagcgcttcc agcaggccaa tccgatcggc
     2821 aaggcagaga agaacgttgc ccatcactac gacctcggca acgactttta tcgtctgttc
     2881 ctcgaccagg gcatgcagta ctcctgcgcc tactttctct ccgacagcga tagcctcgag
     2941 gaggcgcaac agaacaagtt gcggctgttg gccgccaagc tcaatctccg gccaggactg
     3001 agggtcctcg acatcggtag cggttggggc gatctggcgc tctatcttgc gcgcctcgaa
     3061 gacgtcgacg tcaccggcgt caccctgtcg cgcgagcagt acgctctcgc caacgaaaag
     3121 gcgcgggctg ccggccttgg cgatcgagtg cgctttgagt tgcgcgacta tcgtcaggtg
     3181 acggagccat tcgaccgcat cgtctcggtc ggtatgtttg agcacgttgg cgtccgccac
     3241 tatcgcgaat tcttcgccaa ggtgagagcg ctcctggatc ccgacggggt catgctcctg
     3301 cattccatcg ggcacatgtc ccctcccggt accgccagcc cttggctcag gaaatacatt
     3361 tttcccggcg cctattcgcc ggccctctcc gaggtgttca ccgccgtcga gcaggagagc
     3421 ctgtgggtga cagacctcga gttccttcgg ctccattacg ccaagaccct caatcattgg
     3481 tgtcggcgct tcgaagccaa ccgcgccagg atcgcccaga tgtacgacga gcgcttttgc
     3541 cgcatgtggg agttttatct gatcagcgcg gaaatgatgt tccgcacggg cagccagctg
     3601 gtctttcaca tgcagcttgc ccataagcgc gatgcggtgc cgatcgtgcg cgactacgtc
     3661 accgacctgc agcgcaaata ccgcgagacg gaggcccaca tggcgaaccg tcccttcgcg
     3721 aaagccgacg gctaagccgc gctcgccaag ttgtgatgag cggaaaaggc gcgacgctgt
     3781 cgtttttgtt catagggcgc tgccgacaac gttggtccgc atttttgcag cattacggtg
     3841 tcagcatgct cgcgcccccg ccgcgcctgc aactcttgcc tgccatcgcc ctcttgctcc
     3901 cgtgcctggc cgcgaccgcg gaggcgcatg ggggtgggca cggctccggc atgcggtccc
     3961 tcggccgccc ggcagctgcc agcgtgacgg cgccaatgcc cgaacgtacg gcgccgggcg
     4021 cgggcgcggc cgcaaccggc agcgctcgcg ccgatgccat agccaacacc gccgcaacgc
     4081 ccctggccgg gccaacgccg gcaagtccgg cgacgttgcc gggcgcttcg gcaagtggcg
     4141 gcggtcgcgc cgccgccacc gacaattcgg cggccgcggc agcgtggcgg tgcccatacc
     4201 gagcgcgccg gcgactgcgc ccatcaccgg gccggcaacg ccggcgccgg cgctgcccgc
     4261 cctgtccccc atgtcggacc cgttgccgac ccagttcgcc acggggggcg gatcttcacc
     4321 aaacctcgcg ctgtcgccgg ccacgtccgc ctccagcccg agcgaaacgg cgccgagcac
     4381 accgggcggc ggtggtaaat cgctccagga ctgcatgggc ttttgggatg cgggcaccca
     4441 catgacaaaa cccgagtggc gcgccgcctg caaacgctcg atggcggagt ttcccgacat
     4501 taagtggtag tcgccgtcgc gccaccgtcg gaccgaccta ttcgacgcgc agcctgaggt
     4561 ccgtgattaa tttgccccat ttggcccgct cggcaccaac gtaagcggtg aactcggcag
     4621 caccggtgcc gaccgtctca gccaccaggc cggccaggcg ctccttgagc atggcatctt
     4681 ggcaaatgac cttggtgtcg gcttcgattt tgtcgcagat cgccttgggc gttcccgcgc
     4741 gcaccgccac gccggtccac gacgtggtgt catagcccgg gaccgtttcg gcgatggggg
     4801 gaaattccgg ggccagcgtc gagcgctgcg tcaaagtaat gccgagcggc ttcaccgcgc
     4861 cggcacgggc ctggccgatg atcgaggtga tgttgtcgaa catcagatcg acgttttgcc
     4921 ccaccagatc attgagggcc ggcgcgctgc cgcggtaggc aacgtgcacc atgtcgaccc
     4981 ctgtcatgcg cttgaacagt tcggccgaga ggtggaccgt ggtgccgatg ccggaggagg
     5041 catagttgag cttgccggga ttggccttgg catgggcgat aagctcggcc acggtatcga
     5101 ccggcaaatc cttgcgcacg cacagcaggt tcgggacgct ggcgacgtgc ccgagcagca
     5161 caaagtcctt ctcgggatca aatggcaccc gggcgtaaag atacgggttg gtagcgatgc
     5221 cgggcggcac gatgcagatt tgactgccat cgctcggacc cttggcgacc cgatccatgc
     5281 cgagattgcc accggcgccg gcaacatttt cgaccacggt gggcaccttc cacagcgcac
     5341 ccagccggtc ggcgatcacg cggccgacga tatcctggac gcctcccgcc ggaaacccca
     5401 ccacaaactt gaccgtcaac cccgccggat agcccggctg cgccgccgcc cgcgttggcg
     5461 ccgaaacggc aacggccgcc aaaccctgcg caaacgtgcg ccgtgggatg cctgccaaag
     5521 ctgcctcgct ggagaagatc aaagtggtga aggctcggtc tgcaaccgag tgggggggcg
     5581 cgcgggaccc taacacgccg gaaaatgctt gccaggagaa aattccgcgc cctgtatcag
     5641 gccccgcgcg caagcagggt gttattgttg gtgatgccgc tgatgcgata gccgtgcccc
     5701 tggcacagtt tgatgcagtc gacgctccag ctggggcgat agacgtgctc gataataatg
     5761 gcgtcgggcc agtgctcctg aggaacctcg gccaggtagg gaggcagcac gcgatcctca
     5821 taaccttcga cgtcgatttt caggacggtg attttgccaa ccgcgtggtc ggcgaggagg
     5881 gcggcgaggc gcctcacctg cacgggacga ccgacgccgc taccccggag cgatcgtgag
     5941 ccctcgtcac cgctcgtcga aagcaaagcc tcgccgtcat gctcgccgac ggcgacgggg
     6001 acggtggtga cctcgatgtc aggcgcggcg taacgcgcca cgcgggcgag ccgcagattg
     6061 aaggtaaagc gcgccagcag cgccgcgtcg ggttcgattg ccaagatgcg tgcgcccgcg
     6121 cctgcgttca gcgctgcgta gaggctatag aggccggcgt tggcgccgac gtcgacgaaa
     6181 accgaccgcg cccgcgcgag ctgggtgcgc aggaagccgt attcctggcg atccatgcgg
     6241 tcggggcgca atagcgcctt gcgttcgcat acattgtgat cgggaaagag acggatcctg
     6301 tgcccccaca gccagcaatc gaccggaccc gcgtgcagca gaaacaagag ccgcgccagt
     6361 tgtttgcgca gcttgccacg ccccaacagg gtatggcgcg aaagtcctat cagcgcgcgc
     6421 accgtcaggc ccggacgaaa tgtcccccag ggcagtgcgt tcatctcgaa ctcgagcatt
     6481 gagcccatca ccgaaagctc gggccgggga gggcgcgcgc taggcgaagg cgttcgcgtt
     6541 caccgcagcc gttgattgat ggcatcgagc gccgctgccc ccgggcaaag ctgcgacatt
     6601 gcgatttggt tgaggggctg atcggtcgag accagcacgt cgtgcatatc ctgttggctc
     6661 tcgtcgatgt agagcaggcc ggtggcgatg cgtccttgct tgtcgttgcg gcgaatcacc
     6721 atgatggcgg cgtcggcatc gcgaggatcg tggccggcat cgagcttgtg caacaccagc
     6781 cgcgaaccgt cgtgcagtgc aatggcggcc gaactgccct cggcatagtc taccgtgatt
     6841 tcatccctgg gctcgacgta gtcgagcggg ttttccgagg ccatgttgtg cgcgcgcacg
     6901 taatcgaagc ttttggtcga ggtcgggtga ttgttgaagg ttacgcatgg actgacgacg
     6961 tcgaggaagg caaagccctt gtagccgatg gcggccttga tcaatgggat cagctggttc
     7021 ttgtcgccgg aaaaggaacg cgccacgaag ccggcgccaa gttggatcgc aagctgacaa
     7081 aggtcaatcg cctcgaaggg gttggcttca cccttcttgg acggcgaggt cttgtcattg
     7141 gtcgcggaga actgaccctt ggtgagcccg tagcagccat tgttcatgac gatgtaggtc
     7201 atgttcaggc gacgccggat ggcgtgacag aattggccaa ggccgatcga ggcggtgtca
     7261 ccgtcgccgg agacgccgat gtagacgagg tcccgattgg caattgccgc acccgtcgtg
     7321 atcgatggca tgcgcccgtg cacggaatta aaaccgtggc tgcgagacaa gaaataggtc
     7381 ggagccttcg atgagcagcc aatccctgac accttcgcaa tccgatgcgc gggcagatcc
     7441 atctcgaagc aggcctcgat caaggcggcc gtaatggagt cgtgtccgca gcccgcgcag
     7501 agcgttgaca tggtgccctc gtaatcgcgt cgggtcagac ctgacgcgtt cttcggcagg
     7561 gcgggatggt gcgccgaggg tttggcaatg tagctcatgc tcgttcttac tccgctgccg
     7621 cttgggcggg ccgcaactcc ttgagcacgg ccccgcgcac gaaggcggcc gtcatcggca
     7681 tgccgtcgta attgagtgcg cggatcagtt tgccaccggg cacatcgatt tcggtcatca
     7741 gcagcgagcg catctgggca tcgcggttct gctcgacgac aaccaacgtg tcatgatcct
     7801 cacagaaggc gacgatctcc ttgctgaagg gaaatgcctt gatgcgcagg gcgttgatct
     7861 tgatgccatc gcgctcgagc aggtcgagcg cctccagcac ggcgggcgca ttggcaccaa
     7921 agtaaataat gcccatcttg gcgttcttgt cgcgaatgct cacttccggt ttcggcacca
     7981 gcgttgccgc ggtatcaaat ttgcggcgga tccgatcgag gatgcgagca tgcgcgcggc
     8041 cgtcttccgt atagcgtgca ttctcgtcgt gtgaggtgcc gcgcgtgaaa aaggcaccct
     8101 tgctgggatg tgtaccgggc agggtgcggt aggggatggc atcgccgtcg acgtccaggt
     8161 agcggcccca gtcgcgcccc ttgaggtcct tgaagccctc gagcgcggcg gcgtccaaca
     8221 ccttgccgcg gtcgtgcacg tgcgcgtcgt cccacaccag cggctcggtc acccactcgt
     8281 tcatgccgat atcgagatcc gacatgacga tgaccggggt ctgcaggcgc tcggcgaggt
     8341 cgaaagcctg cgcccccatc gagaagcatt cgttgggatt ggcggggaac agcagtacct
     8401 gtttggtatc cccgtgcgag gcataagcac aggaaacgat atcggactgt tgcgtgcgcg
     8461 tgggcatgcc ggtggaagga cccccgcgct gaacattgaa caggaccacc ggcacttcgg
     8521 cgaaataggc caggcccagg aattcgctca tcagggagat gccaggtccc gatgtcgcgg
     8581 tgaaggcccg cgcgccgttc cagccgccgc cgatggcgat gccaatggcg gccaactcat
     8641 cctcggcctg cacgatgccg tagaggcgct tgccttcctt ggtcacgcgc aggcgtcggg
     8701 cgtacctttc gtaggcttcg gccaacgacg tcgacggtgt aatcgggtac caggcgcata
     8761 ccgtggcacc ggcatagacg gcgcctagcc ccgccgcgtc gttgcccgag accatgatct
     8821 tgcccttgga gcacgcagcg gtgcgcacgc gaacagggag cgggcagtga aagtttgcaa
     8881 gggcgaaatc gcggccgaga ctgaccgcct ccatgttggc cgccaccagc ttctccttgc
     8941 ccttgagctg atcgacgatg gcggtcttga gcacgtcgat gtcgagatcc aacagcgccg
     9001 tcagggctcc gacgtagacc atgttcttga agagctggcg ttggcgcggg tcgctccact
     9061 tgtgcgcgca taactgggcg atgggaatgc cgagcacgct aatgtcgtcg cgcggccaga
     9121 cgcgaggcag ggtcgaatca tagagcagcc agccgccggc tacgacctcc gccaagtcct
     9181 ccttgaaggt ctgcgggttc atggccacca tcaggtcgat gccgtcgcgg cgggcgatgt
     9241 agcccgcatc ggacacccgc acctcatacc aggtgggcag cccctggata ttggagggaa
     9301 agatgttctt ggggctcacc gccagcccca tgcggaacaa cgtcttggca aagatggtat
     9361 tggcgctcgc cgagcccgaa ccgttgacgg tggcaaactt caccacaaaa tcgttgatgc
     9421 cgctcatttt gaggtcgatc gctccatcag cgcagatagg cgttgtggtg ggtcagacaa
     9481 gccggtgccg cctgcgcttc gacgtacatg aacttctgca tgtcccaggc gccggtcgga
     9541 caacgctcgg cgcacagccc gcagtgcagg cagacgtcct cgtcttttgc catgatgcga
     9601 ccggtcttga gcgggctcga gacatataga tcttgcgtct tgttgcgcgc cggcacccgt
     9661 agtttgccgc gcaaactgct ttcctcggcg ttggccgtga aattgatgca gtcaaccgga
     9721 cagatgtcca tgcaagcgtc gcattcgatg cacaacggcg ccgagaatac cgtctgcaca
     9781 tcgcagttga ggcaccgttc ggcctccctg aacgccagct ggtcatcgaa gcccagttcg
     9841 acttcgactt tcaggttgcg caaggcctgc gccttgtcga cgtggggcat gcccacgcgc
     9901 cgttgcgcgg cgtagtcatt cttataggac cactcgtgga tgcccatctt ttggctgaac
     9961 agactgctgc ccggggccgg gcgctcgttg agcgacttgc cgcgacaaaa aagatcgatc
    10021 gacacggccg cctggtgccc gtgcgcgacg gcccagatga tattttccgg cccccacgcg
    10081 gcatcgccgc cgaagaagac gccggcccgg gtcgacatca tcgtcgcctt gtcgaccttc
    10141 ggcatgcccc agtcatcgaa ctcgataccg atgttgcgct caatccaggg gaagctgttc
    10201 tcttggccga tggccattaa gacatcatcg caggcaaata ccacctcgtc tccggagggt
    10261 tccagccgcg gccgtccgcg cgtgtcaata ccgacttcgc gcatcagctg gaagcggacg
    10321 ccggcgagct tgccgtgctc gatcacgaat tccttgggcg cgtgattgtc gaaaatcgga
    10381 atgccctcgt gctcggcatc ctcgatctcc cacggcgacg ccttcatcac ccgacgcggc
    10441 gagcgcacgg tcactttgac gtcctcgccg ccgagacgga gcgcggtgcg gcagcaatcc
    10501 atggcggtat tgccgccgcc gatcaccacc acgcgcttgc cgatccggct cacgtgctca
    10561 aaggcgaccg aggtaagcca atcgatgccg atgtggatgt tggccgccac ttgccggcgg
    10621 ccggggactt cgaggtcctt gcccttgggt gcgccggtgc cgatgaagac ggcatcgaaa
    10681 ccttggccca gcaaggcttc gaggctcttg atctcgctgt cgaagtgcgc ctcgaccccg
    10741 aacccaagga tgcggtcgat ctcctcgtcc aggaccgttt ccggtaggcg gaaggaggga
    10801 atgttggtgc gcatcaggcc gccgccgcgc gtatcctttt cgaatagaat gcaagtgtag
    10861 ccgagcggca tcagatcgcg cgccacggtc aaggaggccg gtccggcgcc gaccagggct
    10921 atgcgcttgc cgttcttctc gcgcggcacg tccggcaaga aggcatcgat ctcgcccttg
    10981 tagtcggcgg ccacgcgctt tagacggcaa atggcgacgg gcttttcctc cacgcgcccg
    11041 cgccggcagg ccggctcaca gggtcgatca caaacgcgac caaggatgcc gggaaagacg
    11101 ttgctctccc agttcagcat gtaggccgcc gcatagcgtt tttgggcgat gaggcgaatg
    11161 tattcgggaa caggtgtgtg cgtagggcag gcccactgac aatcgacgac cttgtgaaaa
    11221 tactttggat cggaggtgtt ggttgcggcc atccggccca tgcttgcttg agcgacctcg
    11281 cccgcctgca tttgcgttcc tgccccttgt tcgccttcgc cccaaccgcc tcttctacaa
    11341 gatcaggccg cgttgtccca acagctatga taggatcaaa cgcgattttt caaccgacga
    11401 agggacgttg tcgcgcatct agcacgccga tgcgctttgg tcattgtgca gcgcaaaaat
    11461 gcccgcccgc gaggctgcgg gggaaccggc aacaaacggc tgcgttctcg cttcgatcgc
    11521 agcgggatcg gccgcgaagt tgtgcgtaag tgcgccgccc gtgcgggtcg ctgcttgcct
    11581 tgcggggcga cgcaccggca taggcgtcgt gcttgctttg atcggtcccg gacgcctatg
    11641 caccgacttg cctctggctt gcgcctgatc aggtcccact acgatgccgt cgtggtcggc
    11701 tctggctatg gtgggggagt ggcggccgcg cggctggcgc gggccggcaa acgcgttgcc
    11761 gttctggagc gtggacgcga aatccccgtt ggcgaatttc cacgccggtt ctccgacctg
    11821 cgcaacgatc tgcagctttc tggtcgacag acgcagctgg gctcgccgac ggggctttac
    11881 gacgtgaggc tcggcgagga catgcacgtt ctcgttggct gcgggctggg gggcggctcg
    11941 ctgatcaacg ccggtgtggc gctgcgaccc gacgcgcgtg tctttgagga cccggcttgg
    12001 cccagcgagc ttgcccgcga tccgcttttg gaacagggct acctcgaggc gcgccgctgg
    12061 ctgcggccgg cgcgcgaccc cggcgcgcat gagcgcagca aactcaaggc gctgggcggg
    12121 gccagcgtcg cgctcggcgt aaagcccgtc gcggccccgg taacggtgag cttcgccgaa
    12181 tgcctcaacc ccgccggcat cgcacaaccc gcctgcacgc tgtgtggcga ttgttgcggc
    12241 ggctgcaatg tgggcgccaa aaataccgtt gcgctgacct acctggcgga tgccgtccgg
    12301 cacggggcag agatcttcac gcacgccaag gtccgcgacc tggcgccgga tggcggcggg
    12361 cgctggtgcg tccacttcca ggtccagcca ggggccgatc aggccgccct cgaggccata
    12421 ggcgccgtct cggcggacct cgtggtgctg gccgcgggca ccttgggatc gaccgagatt
    12481 cttctgcgct cgcgcgatcg cgggcttgcc ctgtccgatc gactgggtca ccgcttttcg
    12541 gccaatggcg acatcatcgc cttcggttgg gggggcaggc tgccggtggg tgccatcggc
    12601 gtcggccatc ccgccaagct aaaggacagc gtggtcggcg ccgccgtgtc cggccagatc
    12661 gaaatcgtcg atgccggcga ccttgccaat agcctaacca tacaagaagg cgccctgccg
    12721 tcggccatgg cgccgatcct gccggttctc tttctgccca atgggcgatt gctcggcgcc
    12781 ctgcagagcc tgatcagcgg cgtctacaag ggcccgtttg cccggctgca gacctttttt
    12841 gcggtttccc acgacagcgc ggcaggccaa ctccgtctcg aggccgaccg tgtcgtgctg
    12901 agttggccgg gcgccagcgc tgaacccgtc tatcgccgcc tcgattccgc gctggaagcg
    12961 ctggtttgtg gggcgggcgg cagctatgtg aaaaacccac tggccggcac gatcatggga
    13021 catcaccccg ccacggcaca cccgctcggg ggctgcagca tcgggcacga tcatcgcgac
    13081 ggcgtcgtca accacaaggg tcaggtgttc gcgcccggtg gcgctggcgg cgacgtccac
    13141 gacgggctct acgtcatgga tggatcgatc gtgccgcgct ccctcggggc caatccactc
    13201 ctgaccatta cggccctcgc cgaacgcgcc atggcgcaca tggcgcgcga ccaaggctgg
    13261 cgcgaggcgg ccgcttcggc gctcaacctt ggatccggcc taggccctgg cctggccgcg
    13321 ggcgcacgac aaacgcccgt caggcttccc gcgctcgcct cagccagcca tcatatacaa
    13381 atagagaacg gcgccgatcg cgcccgagat gacgaagccc gcgaggaaac cgcgcacgtc
    13441 ggggcgccgt tgctcggcgc ccagcggcgc tgaaggcaag gcctcggcgg ccctagccgg
    13501 caccgctctc ggaccaacgc tcagcgggac aacggccgtg ctctcggcgg gaggacggtt
    13561 ggtggcgagc tgcgcctgca acagccgctt cagggtctca accgcctcgg cggcggccgt
    13621 ggcgtccgcg gcaagtttgg cagccgcatc aatgcgctca accaggcgcg gtgcggtcgg
    13681 cagcgcggcg gccgtgacgc ccgccagggc cgcctttagc gagctgatcg gggtggtatc
    13741 gaggcggtcg gcggcaattt tttccgcatc gctgagccgc atggaacgag gctgcggggg
    13801 cagctgggga gccgtattgt caatggccaa cgcaggtaaa ctcgacattc ttaagcctct
    13861 acgctgcaac ggtcttgcct agtcccggtc cgtttggctt ggatgccccc ttccgccggc
    13921 cggacctcgc cctggttcgg tttgcacgcc cccctttgcc ggcacggggg acatcgccca
    13981 cccgcctcgc ctactccttg gccaggctgg taatgcgatc aagcaatctc agccggcgag
    14041 gctcctcagg tatgtcgccc cctttaggca tttctgcggc agagaccgcc ggtctttggc
    14101 ttgggccttc cacgtccacg ccgttgcgga ccggggcagg cgggccctcg agcttttcag
    14161 gttcgggcgc accggcctct ttggcgctgc tgcgcgggcc cggggtggtg gatctcacct
    14221 gcgcgacgcg ttccgccaat ggccggcgat cggcgcgggc gctcgggcgc ggctgcgcgg
    14281 cgacggggag ggtgccagcc ccaagccggc gcatctcgtc catgaagtgg gccgcctggc
    14341 gggcgagccg ctcgttgacg gcggcaagct cggcccgcag cctgccgacc atggccgact
    14401 ggtgatcggc ctgcgcttgt gccgagctta aacgtgcctt gagcgcggca cggctctcct
    14461 tcgaagcccc ctgggtcccg ctctccccct cgaaggttgc cagcgccgcc ctgaggcggg
    14521 tgatctcgcc ggcctgatca tcggccttgg tcttaaggag gcgcagctcc gcttcgagcg
    14581 gggtgccctt gtccggctcc gccgcgccgg agcgcgccga ggcgagcgcg gcttccgcct
    14641 gggccagcac ctcttgcgag cggcttgggg tggcctcgac ggccgatacg ccggcggagg
    14701 ccaccacctg cggttcgctg gcactgcgtt gcaggcgatc gatcacgttg gcctggtcgc
    14761 gggccttcgc gcgcaacgcc tcgagctcgc tgcggagtgc gacctcggct tcaccgcccg
    14821 catcgcccgc ttgcttgctg cgagcgctgc gggcggcgac cgcgctcgtt aggcgttcga
    14881 tctgagaggt ctgctgggcg ttgatggagg cggcctcctc cagggcgagc ttgtggcgct
    14941 tggcattttc caacagctcg gcgatttcac gctcgcgact gatgatgagg cgcttggcct
    15001 cgccgaggcg tgcctccacc ttgggtagcc ggtcggcaat cgtttgctcc aacacgtgcc
    15061 tggcattttg gtgctcttcc aattcggctt tgagctcgcc gacgttggtc tccaacgtcg
    15121 agatgctggc atcgcgccga ttgagttcga cgagctggcg cgcgcgggcg agcttggcct
    15181 gctcgaggtg ggtctcgagc ttgtggacct tgatcgcata ctcggcccgc agccggtcgc
    15241 gatcggcctg aatttcctgt tcgctcacgg gcagggactg cttgatgcgc gccgtcgtca
    15301 gacgcacggc acgaccccag aaggccgatg ccagaagcag tccaagcaga ctggccgcca
    15361 ggaagcccaa agccacgagc atggcggatt ggatggaaat caagccctta tccttcggtc
    15421 ggccgaggtg cggcgacaac aactacacta cgcgcaaccc ccgcacctgg atccaacgct
//
LOCUS       DASTMT010000345         1660 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_2484374, whole genome shotgun sequence.
ACCESSION   DASTMT010000345 DASTMT010000000
VERSION     DASTMT010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 1660)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1660)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1660
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2484374"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..319
                     /locus_tag="VFR00_08125"
     CDS             <1..319
                     /locus_tag="VFR00_08125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014496506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HEU0159266.1"
                     /translation="VVEHMADRVAVMYLGRIVEIADKEELFARPRHPYTRALISAAPL
                     PDPGAKRERIVLEGDVPSPMSPPRGCHFHTRCPFAVARCRADYPELRSAGPMHHFACH
                     LDL"
     gene            515..1387
                     /locus_tag="VFR00_08130"
     CDS             515..1387
                     /locus_tag="VFR00_08130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0159267.1"
                     /translation="MRMAAQIGLTVVVIIAGAAAIGVLESLYGQGLHRVIGWVIVALL
                     ILLIWLPKWPVSQKNIATANNALFAEHALGFIELTPRNPSAHQLGQQLISTMRRLGTG
                     LANDTAVIEDFNGKNRLAQLNLIAMAMIDLKQSPMLGEVWETPNAQATEGASARAVAA
                     ATEAIFGKHGIAITVPERRFRIEGGCLLDAGDLAPPPKSPPPPPDLMRQAIYGAFAFF
                     LEQTKDEKVDFNEYHLLVLKSYVTAISIPISTAEPIVAMARLADLPKGWEQWAVSAIV
                     DELFRMVHDQIYKK"
BASE COUNT          293 a          538 c          537 g          292 t
ORIGIN      
        1 cgtcgtggag cacatggccg accgcgtcgc cgtcatgtat ctcggccgta tcgtggaaat
       61 cgccgacaag gaggagctgt tcgcacggcc gcgccatccc tatacgcgcg cgctgatttc
      121 ggcggcgccg ctgcccgacc cgggggccaa gcgcgaacgc atcgtcctcg aaggcgacgt
      181 gccctcgccg atgtcgccgc ccaggggttg tcatttccat acgcgctgtc cgttcgcggt
      241 cgcgcgttgc cgcgcggact atcccgagct ccgaagcgcg ggcccgatgc accacttcgc
      301 ctgccatctc gacctttgaa gccttgcgcc aagcgtgcgg ggccgcgcca caattgcgcc
      361 caggggccct gggcagggga tggtcagccc cgggtgcacc ggcggcagcc cagccgcatc
      421 ttcgcgaggg ggtagcgagc cacgagaaag gcctggaccc tcacccaatc ggtcaactag
      481 gcttcggcgc gaggtcatga gggaaaagcc gcgcatgcgg atggctgccc agatcggctt
      541 gacggttgtc gtcatcattg cgggcgccgc cgccatcggc gtgctcgaaa gtctctacgg
      601 gcaaggcctg caccgggtga tcggttgggt cattgtcgcg ctgttgattt tgttgatttg
      661 gctgcccaaa tggccggtca gccagaaaaa catcgccacc gccaacaatg cgctgtttgc
      721 cgaacacgcc ctcgggttca tcgagctcac gcccaggaac ccgtcagcgc atcaattggg
      781 gcaacagctg atcagcacca tgcgccggct cggcacgggg ctcgccaacg ataccgccgt
      841 gatcgaggac ttcaacggca agaaccggct tgcgcaattg aacctgattg ccatggcgat
      901 gatcgatctc aaacaatcgc ccatgcttgg ggaagtctgg gagacgccga acgcccaggc
      961 caccgaggga gcttcggcgc gcgccgtcgc cgccgctacc gaggcgattt tcggcaagca
     1021 cggcatcgcc atcaccgttc cagagcggcg ctttcggatc gaggggggtt gcctgctcga
     1081 tgccggcgat ctggcgccgc cgccaaagtc accgccgccg ccgccggatc tcatgcgtca
     1141 ggccatctac ggcgccttcg cattctttct cgaacagacc aaggatgaga aggtcgattt
     1201 caacgaatat cacctgctgg tgctcaaatc ctacgtcacc gccatctcga taccgatcag
     1261 cactgcggag ccgattgtcg ccatggcccg acttgccgac ctgcccaaag ggtgggagca
     1321 atgggcggtg agcgcgatcg tcgatgagct atttcggatg gtgcacgacc agatctacaa
     1381 gaagtaatgg ggctgtcggc gacgcaagct gcgccgggga accttggctg gctcgagccg
     1441 tttggccgtc agacgggccc ccacggcctg ttaggcgccc cacaagggtg ctgagccgcc
     1501 cagtaacctt cgggtgatcg gcggctttta atttgctggc aaggcaagga ccggccccgg
     1561 cgatggcgaa gctgccgttg caatgccact gcggctgagg gggccttttt cccccgggca
     1621 cggcagggtt tggcgggggg tgcgggaccc tagccatggg
//
LOCUS       DASTMT010000346         1940 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_2814994, whole genome shotgun sequence.
ACCESSION   DASTMT010000346 DASTMT010000000
VERSION     DASTMT010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 1940)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1940)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1940
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2814994"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..581)
                     /locus_tag="VFR00_08135"
     CDS             complement(<1..581)
                     /locus_tag="VFR00_08135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011924306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU0159268.1"
                     /translation="MTDVAWIEAVLTSARAQAVAALLRYFRDLDTAEEAFQEACLRAL
                     KSWPRNGPPRDAAAWLIMVGKNAAIDTVRRQRREEPLPQEELISDLSDAEATTVERID
                     AADYRDDILRLLFVCCHPDLPATQQIALALRIVSGLTTRQIARAFLVSDDAMEQRITR
                     AKARVAKANVPFEAPSAVDRSERLAAVAAMLYL"
     gene            complement(578..949)
                     /locus_tag="VFR00_08140"
     CDS             complement(578..949)
                     /locus_tag="VFR00_08140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008961694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEU0159269.1"
                     /translation="MQYAILCYHDENVVCAWSKQEDDAVMARLAVVQERLGRQGKLGP
                     VVRLLPTTAATTLRKDREPPLVMDGPFAETKEQLLGFYVVDCSSLEEVLEIARELGAA
                     NPGGAYEIRPIGVFNPGSARA"
     gene            1298..>1940
                     /locus_tag="VFR00_08145"
     CDS             1298..>1940
                     /locus_tag="VFR00_08145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0159270.1"
                     /translation="MFLTHGLAAAPALAQGTASAAPPAEKVIVSPPPQQGSRLRAALS
                     KLLGKDQREQLGSSKSEVWTVPQARVEQLKARFKELGLKVTQLREDWNHILKRQQGPL
                     TMTPAQEAAIGKAQGAREVVGVGLMRAPEAAVAEYALTAGEKKKGKGALPEEAQGRIV
                     IPLNDKHHITVERTKAISTEKGTTWRGKVSETGESAVLMWWKDGRLSGMFGYKG"
BASE COUNT          355 a          617 c          655 g          313 t
ORIGIN      
        1 aacaggtaga gcatcgcggc cacagcggct aagcgctcgc tgcggtcgac ggcgctcggc
       61 gcttcgaatg gcacgttggc cttggcgacc cgggccttgg cgcgggtgat gcgctgttcc
      121 atggcatcgt cactgaccag gaacgcccgg gcgatctggc gcgtggtaag ccccgagacg
      181 atgcgcagag ccaaggcgat ctgctgggtt gccggcaggt ccggatgaca gcacacgaac
      241 agaaggcgca agatgtcgtc acggtagtcg gccgcatcga tgcgctccac ggtggtggcc
      301 tccgcatcgg acaggtccga aatcagctct tcctgcggca gcggctcttc gcggcgttgc
      361 cggcgcaccg tgtcgatcgc cgcattcttg ccaaccatga tcagccaggc ggcggcgtcg
      421 cgcggtggcc cgtttctcgg ccacgacttg agcgcccgca aacatgcctc ctggaaagct
      481 tcctcggccg tgtcaagatc gcggaaatag cgtagcagcg ccgccaccgc ctgggcgcgg
      541 gcggaggtga ggacggcctc gatccacgcc acgtcggtca cgcgcgggcg ctcccgggat
      601 tgaatacgcc gatcggtcgt atctcgtagg caccccctgg gttggcggca cccagttcgc
      661 gcgcgatctc gagcacttct tcgagcgagc tgcaatcgac cacgtagaag cccaacagct
      721 gctctttggt ttccgcgaac gggccatcca tcacgagggg aggctcgcgg tccttcctga
      781 gcgtggtcgc cgcggtggtt ggcagcagcc gtaccaccgg tccgagcttg ccctgcctgc
      841 ccaaccgttc ttgcacgacg gcaagtctgg ccatcacggc atcgtcttcc tgtttgctcc
      901 aagcgcacac gacattctcg tcgtgatagc aaagtatggc gtactgcatc ggcagccctc
      961 ctgctgtctg cttcccaagg acgtttgagc cgggctgatc ccgacaggta aaatgcgctt
     1021 cacatgggat tgttctggat caggcgatcc aacccctcaa ctcggccagg atccaaccta
     1081 agtgtgttag actgcaacac ccgcatcgct ggccgcggca tggcagcgcg gcgcgtgggg
     1141 cggagatggg gatcagctca caaggcttgg cgggaaaatg ctcagttcga tccgatgctt
     1201 gaccacggcc gtgccgccaa gccggcggcc taacggagaa gccgactgcg cgaagagaca
     1261 acgcccgctg cgaccggtga tcctggcgct tgcggccttg ttcctcacgc atgggctggc
     1321 cgcggcgccg gccctggcgc agggcacggc ctcggcggcg ccgcccgccg agaaggtcat
     1381 cgtcagcccg ccgccgcagc agggctcgcg tctgcgcgcc gcgctctcca agctgttggg
     1441 caaggaccag cgcgaacagc tcgggtccag caaatctgag gtgtggacgg taccgcaagc
     1501 gcgcgtcgag cagctcaagg cccgattcaa ggaattgggc ctcaaggtga cgcaattgcg
     1561 cgaggactgg aaccacatct tgaagcgcca gcaggggcca ctgacgatga ccccggcgca
     1621 ggaagcggcg attgggaagg cgcaaggcgc gcgggaagtc gtgggcgtcg gactgatgcg
     1681 ggcgccagag gccgcggtgg cggagtacgc cttgaccgcc ggcgagaaga agaagggcaa
     1741 gggtgcactg cccgaggaag cccagggtcg catcgtcatt cccctcaatg acaagcacca
     1801 cattacggtt gagcgcacca aggcgatcag cacggaaaag ggcacgacct ggcgcggcaa
     1861 ggtcagcgaa accggcgaaa gcgccgtgtt gatgtggtgg aaggacggcc gcttgtccgg
     1921 gatgttcggg tacaagggcc
//
LOCUS       DASTMT010000347         3616 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_1832491, whole genome shotgun sequence.
ACCESSION   DASTMT010000347 DASTMT010000000
VERSION     DASTMT010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3616)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3616)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3616
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1832491"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..229
                     /locus_tag="VFR00_08150"
     CDS             <1..229
                     /locus_tag="VFR00_08150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018320050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HEU0159271.1"
                     /translation="LFVAISMTAGGGAWDNAKKYIEDGHFGGKGSEAHKAAVTGDTVG
                     DPYKDTAGPAVNPMIKITNIVALLLLAVLAH"
     gene            complement(247..750)
                     /gene="bamE"
                     /locus_tag="VFR00_08155"
     CDS             complement(247..750)
                     /gene="bamE"
                     /locus_tag="VFR00_08155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HEU0159272.1"
                     /translation="MRYERPAQHQPPEAGAVRVAPRRWLRAAAVGLLIALGLSACAET
                     LTKRGHQFHDNDLKQIQPGMSQEQVKTSLGTPATTATVGSGSAYYYISSVEGQTSFFA
                     PTVKDRQVVAIYFTSLGAVDKVAQYGLKDGKVFDYIKRETPSHARDEGILKALFRNLG
                     TKQLGLD"
     gene            957..1427
                     /locus_tag="VFR00_08160"
     CDS             957..1427
                     /locus_tag="VFR00_08160"
                     /inference="COORDINATES: protein motif:HMM:NF015914.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome C chaperone family protein"
                     /protein_id="HEU0159273.1"
                     /translation="MTQARAPIFYSAWGVPDTLAGRCEMLILHLSVVLDRLGREGADG
                     AALARALSEAFIADMDGNMRELTFGDLAVPREIKRTAAALFDRHGAYRQAFAAGQPGP
                     LQEAIEAQMAYLAPAGRLDAARLAHYMHEVARLITRQSGSALLAGRLGWPAPSA"
     gene            1522..2043
                     /locus_tag="VFR00_08165"
     CDS             1522..2043
                     /locus_tag="VFR00_08165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEU0159274.1"
                     /translation="MPGELSWDHLVEDIPVTGLSVERLADAEERERLRLALDLVGMPE
                     LRARYQLTPVSYGRYELVGELAAVVDQTCVATLEPVRSEVAETYTATYWPPEAMPGPI
                     KGQVALDAQPDPEAIVDGSIDVGRIVFESLAAAINPFPRLPGAVFEGPLSTPEGQKPE
                     SPFAVLANLKAKS"
     gene            2222..3274
                     /gene="plsX"
                     /locus_tag="VFR00_08170"
     CDS             2222..3274
                     /gene="plsX"
                     /locus_tag="VFR00_08170"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009209307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEU0159275.1"
                     /translation="MTRPRTISLDAMGGDGGPGVVVPGAALALKRQPDLRFLFFGARP
                     RVEAELAAHKDLSACSSIVHTDIAVEMQDKPSQAVRRGRGSSMWLALEAVQKGEADCM
                     VSAGNTGALMAMAKLCLRTMPGIERPAIAGQWPTISKPCIVLDLGANIGATARQLADY
                     ALMGAAMARALFHVERPSVGLLNIGVEEIKGVEEVRQAHAWLKQSNLQIDYRGFVEGD
                     QIGQRVADVVVVEGFAGNMALKTAEGTARQMAAYVREALKSSWAARIGAVFAARGLSA
                     LKDRMDPGRLNGGPFLGLNGIAIKSHGGTDTFGYASAINVGYQMVESNVLERLAADLD
                     AFHGNLHEVEAARKTS"
     gene            3357..>3616
                     /locus_tag="VFR00_08175"
     CDS             3357..>3616
                     /locus_tag="VFR00_08175"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084779.1"
                     /note="FabH; beta-ketoacyl-acyl carrier protein synthase
                     III; catalyzes the condensation of acetyl-CoA with
                     malonyl-ACP to initiate cycles of fatty acid elongation;
                     differs from 3-oxoacyl-(acyl carrier protein) synthase I
                     and II in that it utilizes CoA thioesters as primers
                     rather than acyl-ACPs; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP synthase"
                     /protein_id="HEU0159276.1"
                     /translation="MAFIRAVIRGVGAYLPKKVLSNHDLSRVVDTSDSWIRERTGIEQ
                     RHIAADDELTSDLGIAAARQALVRAGVDPVDVDLIVCATATP"
BASE COUNT          607 a         1133 c         1258 g          618 t
ORIGIN      
        1 cctgttcgtg gccatctcca tgaccgcggg tgggggcgcc tgggacaatg ccaagaagta
       61 catcgaggat ggccacttcg gcggcaaggg ctcggaggcg cacaaggcgg cggtgaccgg
      121 cgatacggtc ggggacccct acaaggatac ggcgggcccg gcggtcaatc cgatgatcaa
      181 gatcaccaac atcgtggcgt tgctgctgct ggccgtgctg gcgcactgaa ggggggcgcc
      241 gcggcctcag tccaagccca gctgcttggt ccccaagttc cggaacaggg ccttgaggat
      301 gccctcatcg cgcgcgtggc tcggcgtctc gcgcttgatg tagtcgaata ccttgccgtc
      361 cttgaggccg tactgggcga ctttgtccac cgcaccgagg gaggtaaagt agatggcaac
      421 gacctggcga tccttgacgg tgggcgcaaa gaacgaggtc tgcccctcga cgctggagat
      481 atagtagtag gccgatcccg agccgacggt ggccgtcgtg gcgggcgtgc caagcgaggt
      541 tttcacttgc tcctggctca tgccaggctg gatctgtttg agatcgttat cgtgaaactg
      601 gtgtccgcgt ttggtgaggg tctccgcaca ggcgctgagc cccagcgcga tcaggagacc
      661 caccgccgcg gcgcgaagcc agcgtctggg cgcaaccctc acggcgccgg cctcgggcgg
      721 ttggtgttgc gcagggcgct cgtagcgcat gatagagcgt ccctcatgag aatcgactgc
      781 agagccccgg ccgtggccta acgcaggaag cttttatttg caacgctggc agcgccgccc
      841 tccctggcgc tgcccgcatc tcgagacgag cccggagcat ccttgctgct atgtttgcct
      901 ggctgtcgaa acggttggac cggcgccgga ctgcacgcga gctttatggc tcgattgtga
      961 cgcaagcgcg ggcgccaatc ttctattccg cctggggtgt cccggacacg cttgccggcc
     1021 gctgcgagat gctcattttg cacctgagcg tcgtcctcga ccggctcggc cgagagggtg
     1081 ccgacggcgc cgccctggcg cgcgcattga gcgaggcctt tattgccgac atggatggca
     1141 atatgcgcga gctgaccttc ggcgatcttg ccgtcccccg ggaaatcaag cgcacggcgg
     1201 cggccctgtt cgaccggcat ggcgcctatc gccaggcgtt tgcggccggc cagcccgggc
     1261 ctttgcagga ggcgatcgag gcgcaaatgg cctacctggc gccggccggg cgattggacg
     1321 cggcgcggct tgcacactac atgcatgagg tcgcgcggtt gatcacccgc cagtccggca
     1381 gcgcgctgtt ggcgggacgg ctgggctggc cggcgcccag cgcttagaaa agcgtgtggg
     1441 cgcgggccga ctcgaaggat catcgcgcag gatcagaacg aggcgtcagc tcgcaaaggc
     1501 gcggcgcgga aaagaggcag catgccaggc gaactcagct gggaccatct cgtcgaggat
     1561 attccggtaa cgggcctcag tgtcgaacgg ctggccgacg ccgaggaacg cgagcgcctg
     1621 cgcttggctc tcgatctcgt gggcatgccc gagttgcggg cgcgctatca gctcacgccc
     1681 gtaagctatg gccgctatga gcttgtcggc gagcttgccg cggtcgtgga ccagacctgc
     1741 gtcgccaccc tggagccggt gcgcagcgag gtcgcggaga cctatacggc gacctactgg
     1801 ccgcccgagg ccatgcctgg gccgatcaag gggcaggtgg cgctcgatgc ccaacccgat
     1861 cccgaggcga tcgtggacgg gagcatcgat gtcggccgca tcgtctttga aagcctcgcc
     1921 gcggccatca atcctttccc gcgtctgccg ggcgccgtgt tcgaggggcc actctcgacg
     1981 ccggaggggc aaaagccgga aagtccgttt gccgtgctgg ccaatctcaa ggccaaaagc
     2041 tgaggcccac ccgggggcgg cagggtggct ttctcggcgc agtcaagtct gcgaaaatgt
     2101 attgtttcgc cacggcaacc ggctatggtc gagcggtttg gcggcctcga gtaggtggat
     2161 ttgctaaaga cctgctggcc cggatctggg ccgcgtcaac caaggtagac aagatcgggt
     2221 catgacacgg ccgcgcacga tatcgctcga tgcgatgggt ggagatggcg gtccaggcgt
     2281 ggtcgtgccg ggcgccgccc tggcgctcaa gcgccaaccg gatcttcgct tcctgttctt
     2341 cggtgcgcgc ccgcgcgtgg aggccgagct cgcggcgcat aaggacttga gcgcctgctc
     2401 ctccatcgtg cacaccgata tcgccgtgga gatgcaggac aagccgagcc aggccgtgcg
     2461 ccgcggccgc ggttcgagca tgtggctggc gctggaagcg gtgcagaagg gcgaggcgga
     2521 ctgcatggtc tcggccggca acaccggggc gttgatggcg atggccaagc tctgcctgcg
     2581 caccatgccc ggcatcgagc gtccggccat cgccgggcag tggcccacca tcagcaagcc
     2641 ctgcatcgtc ttggatctgg gtgccaatat cggcgccacc gcgcgccagc tcgccgacta
     2701 cgcgctgatg ggcgcggcga tggcgcgcgc gctgttccat gtcgagcgcc cgagcgttgg
     2761 cctcctcaac atcggggtgg aggagatcaa gggtgtcgaa gaggtgcgcc aggcgcacgc
     2821 ctggctcaag cagagcaact tgcagatcga ctatcgcggc ttcgtggaag gcgaccagat
     2881 cggccagcgc gtcgccgacg tggtggtggt ggagggcttc gccggcaaca tggcgctgaa
     2941 aacggcggag ggcaccgccc ggcaaatggc ggcctacgta cgcgaagcgc tgaagagttc
     3001 gtgggccgcc cgcatcgggg ctgtgtttgc cgcgcgcggc ctcagcgcgc tgaaggaccg
     3061 catggatccc ggccgtctca acggcggccc cttcctgggc ctgaacggga ttgccatcaa
     3121 gagccacggc ggcaccgata ccttcgggta tgcgagcgcc atcaacgtcg gctatcaaat
     3181 ggtggaaagc aacgtgctgg aacgattggc ggccgacctc gatgccttcc atggcaacct
     3241 gcacgaggtc gaggcggcgc gtaagacttc atgacgtttc aaatcagggt tggtgcggag
     3301 ggtgtttgcc cgaggggtcc ctggctggct tggatgcgac gtggacgagg tgagacgtgg
     3361 ccttcattcg agccgtgatt cgcggtgtcg gcgcctacct gccgaaaaaa gtgctgagca
     3421 atcacgacct ctcgcgcgtg gtcgacacca gcgacagctg gattcgcgaa cgcaccggca
     3481 tcgaacaacg acacatcgcg gcggatgacg aactcacctc ggatctcggc atcgccgccg
     3541 cccgccaggc gctggtgcgc gccggcgtcg atcccgtcga tgtggacttg atcgtttgcg
     3601 ccaccgccac ccccga
//
LOCUS       DASTMT010000348         1756 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_2485810, whole genome shotgun sequence.
ACCESSION   DASTMT010000348 DASTMT010000000
VERSION     DASTMT010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 1756)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1756)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1756
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2485810"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..691
                     /locus_tag="VFR00_08180"
     CDS             <1..691
                     /locus_tag="VFR00_08180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020096352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEU0159277.1"
                     /translation="VTPKTSLIILNSPNNPCGGAVPAEEVKRLVKGLEKHPHVTVLSD
                     EIYSQMLYGGRKHESFLRYPEMRDRLIVLDGWSKTYAMTGWRLGFSVWPQALIEHVVR
                     LCVNSHSCVNAPTQYAGIAALEGPQDEVHKMVAAFDERRRTIVPLLNQLPGIRCLDPG
                     GAFYVFPNVTGTGMSAKDLQNTMLEKAGVATVAGTSFGIHGEGYIRFSYANSVENIRE
                     AVRRLATLLRD"
     gene            698..1450
                     /locus_tag="VFR00_08185"
     CDS             698..1450
                     /locus_tag="VFR00_08185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018646848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU0159278.1"
                     /translation="MDLKIRGKTALVTGASIGIGRGIALALAREGVRLALVARRAQLL
                     DEVAAEIVALGAGAPVLITADLMQEGAPARIIGETLAGLGCVNILINNAGGSRPFKLD
                     ATEEQWHEAMTLNFTRQRQLTHGLLAHMIAANWGRIITMTGKSEPEGINGAFSAKAAV
                     HAWAKGLSREVGKHGITVNCIPPGRIMSEQIRRNYAPEYRQWQADHEIPVGRYGEPED
                     VANLVCFLASPLAAYITGTVIPVDGGLRRYQF"
BASE COUNT          331 a          567 c          552 g          306 t
ORIGIN      
        1 ggtcacgccc aagaccagcc tgatcattct caatagcccg aacaatccct gcggcggtgc
       61 cgtgccggcc gaggaggtga agcgccttgt caaggggctg gaaaaacacc cgcacgtgac
      121 ggtgctgtcg gacgagatct atagccagat gctctacggc gggcgcaagc atgaaagctt
      181 cctgcgctat ccggagatgc gcgaccgtct gatcgtgctc gacggctggt cgaagacgta
      241 tgccatgacc ggctggcgcc tcggtttttc cgtttggccg caggcgttga tcgaacacgt
      301 cgtgcgcctg tgcgtcaaca gccactcatg tgtcaacgct cccacgcagt atgccggcat
      361 cgccgcgctc gagggaccgc aggacgaggt gcacaaaatg gtggccgctt tcgatgagcg
      421 tcgccgcacc atcgtccccc tgctcaatca attgccgggc attcgctgcc tcgatcccgg
      481 cggcgccttt tacgtattcc cgaatgtcac gggtaccggt atgagcgcca aggatttgca
      541 gaacaccatg ctggaaaagg caggcgtcgc caccgtggcc ggcaccagtt tcggcattca
      601 tggcgaaggc tacatccgct tttcctacgc caattcggtg gaaaatatcc gcgaggccgt
      661 gcgtcggctg gcgacgctgc tcagggatta aatctgcatg gacctcaaaa ttcgaggtaa
      721 gacggcgctc gtgaccggtg ccagcatcgg catcggtcgt ggcattgccc tggcgctggc
      781 acgcgagggc gtccgcctgg cgctggtggc gcgtcgcgcc cagctgctcg atgaggtcgc
      841 cgccgagatc gtggcattgg gtgcgggcgc accggtgctg atcacggccg acctgatgca
      901 agagggggcg ccggcgcgca tcatcggcga gaccctggca ggattgggct gcgtcaatat
      961 cctgatcaac aatgccgggg gtagccggcc cttcaagctc gacgccaccg aggagcagtg
     1021 gcatgaggcg atgaccctca acttcacgcg tcagcgccag ctgacgcacg gcctgctcgc
     1081 gcacatgatc gccgccaact ggggtcgtat catcaccatg accggcaagt ccgagcccga
     1141 gggcatcaac ggtgccttct cggccaaggc ggccgtgcat gcgtgggcca aagggctctc
     1201 gcgcgaggtg ggcaagcacg gcattaccgt caattgcatc ccacccggcc gtatcatgtc
     1261 ggaacaaatc cgccgcaact acgcgcccga gtatcggcaa tggcaggcag accacgagat
     1321 ccccgttggc cgctatggcg agcccgagga tgtggccaac cttgtctgct tcctggcctc
     1381 accgctggcg gcctacatta ccggcaccgt gatcccggtc gacggcggcc tgcgccgtta
     1441 ccaattctaa aaaaacggcg cctgtccctg ccgtcggcgc ttggcgctta agggcattta
     1501 atgaacgctt cttaatgttt ggccggcgac ggggcctgga gccttcggcc ggtcccgcaa
     1561 gaacagatca cgagccgggc gcaagccctc aacggagata acccgaaatg aagatgcgaa
     1621 cccgatatgt ggcccccgcg ctgttggcag cggcggccat cccgtcaatc gttcttgccc
     1681 agagagcggc gccgcaggac acccaaaccc gatccgagcg gcaggtccag cggccgcagc
     1741 tgtcgccgca ggcgcg
//
LOCUS       DASTMT010000349         4388 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_1833051, whole genome shotgun sequence.
ACCESSION   DASTMT010000349 DASTMT010000000
VERSION     DASTMT010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4388)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4388)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4388
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1833051"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(136..1011)
                     /locus_tag="VFR00_08190"
     CDS             complement(136..1011)
                     /locus_tag="VFR00_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU0159279.1"
                     /translation="MAKLIEAWRRRYWRADPAALAAYAGLRPFTVVTGGSEGIGLALA
                     RRFAGAGNDVLLVARRPEPLAAAAERIRAESRVEVATASVDITRPDAIAALEAALAAR
                     GAYADILVNSAGIGLAGRFGEQSPEALAQLLDLNVRALTTLSRHFLAGMRVRGRGGIL
                     NLASLGGYAPGPHQAAYYASKAYVLSLSEAVAAETAGEGVRVCALAPGPVNTRFHARM
                     GAEGALYRALVPPASAEAVARVGYLGFLLGWRVTIPGLINPFLALALRVMPHRLVIPI
                     VGWLLQPRGQNAPHA"
     gene            complement(1041..1952)
                     /locus_tag="VFR00_08195"
     CDS             complement(1041..1952)
                     /locus_tag="VFR00_08195"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEU0159280.1"
                     /translation="MRRDIDIALLRAFVAVVETGSVTGAAALLNLTQAAVSQQLKRLE
                     ELFGTQLFERHHKRLSLRPDGERLIVHAHRLIAHNDEIWGAMSAPAYEGEVWLGVPTD
                     IVGVFIPPILKRFDKAWPRVRVSLKCSTTPQLLEALRKGDIDLTLTTEQACRPHGETL
                     LEDDLVWAGASNGVAHRRDPLPISLGRETCEFRPSVLKALRDGKRDWRPVCESGMDAL
                     KASLDADVAVGPFLRRTIPDYLRMVDDPRLPRLPRFLINMYLPAASQSEIAVELARHI
                     RAEFDLRFRRTNAPERRKTMPVAALAS"
     gene            2073..2306
                     /locus_tag="VFR00_08200"
     CDS             2073..2306
                     /locus_tag="VFR00_08200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012332198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1127 domain-containing protein"
                     /protein_id="HEU0159281.1"
                     /translation="MAHLAFPLAAASGAAAQFGGWLSRALLSFELALQVRKERRALQH
                     LGDAGLKDIGLSRADVAAEWHRSFWDVPVDRLR"
     gene            complement(2348..3562)
                     /locus_tag="VFR00_08205"
     CDS             complement(2348..3562)
                     /locus_tag="VFR00_08205"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003615526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HEU0159282.1"
                     /translation="MPGPHTIMTGYAPIKAWIDGVPIEDAARRQLFNIAALPFIHHHV
                     AAMPDVHLGKGATVGSVIASRAAIVPAAVGVDIGCGMMAVRSSLTAAQLPDSLAALRL
                     GIEAAVPHGGVGLKGGWKDGVPARVARLFSTSGLQDGLQELEEKHPALRQAQGLTHLG
                     SLGGGNHFIEICLDESDRVWAMLHSGSRGIGNRIGSYFISKAREALDRRQLTRPLPDR
                     DLAWLEEGEPLFEDYVAAIAWAQEFARRNREIMMAAVLGVMRAQLPPFRTERHAINCH
                     HNYVAREQHFGETVWVTRKGAVRAGRGDLGIIPGSMGAKSYIVRGKGHPESFQSCAHG
                     AGRRMSRGEAKRTFSLNEHAAATAGVECRKDAGVIDETPMAYKDIDAVMAAQDDLVEI
                     VHRLRGIVCVKG"
     gene            complement(3601..>4388)
                     /locus_tag="VFR00_08210"
     CDS             complement(3601..>4388)
                     /locus_tag="VFR00_08210"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEU0159283.1"
                     /translation="LRWLLVALLIGVFAGRHVVRDLPALRRSLGLMLFLALMGGSVFG
                     TMQYVTLQYTTALNMGVVGSISPVFIVAGSWLMFGDRLGGLQLFGVGVSLLGVLAIVS
                     QLSLQRLLALNFNFGDLLIIINMVFWAVYCACLRLRPNVHALSFLFVFALASALFIVP
                     FAAWEFAVGWRLAFEPLTIFAIVYAALVTSLLAYLAWNRGVELIGAPRASAFLHTIPL
                     FAAVFATSLLGERLYAFHVVGFLLILAGVSLAARPSAKLARAG"
BASE COUNT          830 a         1454 c         1441 g          663 t
ORIGIN      
        1 aaccattggc cgacgtgacc gggattggaa ttctcctggt gcaagatgat gcagaccggt
       61 ggcttgcctg gatttgtcgc ctgcgccggg atctggccga cggcccggtc ttgcgagcgc
      121 tcgcagctgc tggcgtcaag catggggtgc gttctgtcct cgaggttgca gcaaccatcc
      181 gacaatggga atcactagtc tatgcggcat gacacgcagg gcgagcgcca agaaggggtt
      241 gatcagtcct gggatggtga cgcgccagcc caagaggaag ccgagatagc ccacgcgggc
      301 gaccgcctcg gccgaggcgg gcgggaccag ggctcgatag agcgcccctt cggcgcccat
      361 gcgggcgtga aagcgtgtgt tgaccgggcc cggcgccaag gcgcacacgc gcaccccttc
      421 gccggccgtc tcggcggcga ccgcctccga caaggacagc acgtaggcct tgctcgcgta
      481 gtaggcggcc tgatgcggcc ccggcgcata gcccccgagc gaggccaagt tcagaatgcc
      541 gccgcgccca cgcacgcgca tgcccgccag gaaatgccgg ctgagcgtcg tgagcgcccg
      601 cacgttgagg tcgagcaact gcgccaacgc ctcgggcgac tgctcgccga accggcccgc
      661 caggccgatg ccggcgctgt tgacgagaat gtcggcgtaa gccccgcgcg cggccagcgc
      721 cgcctcgagc gcggcaatcg catcgggccg ggtgatgtcg accgacgcgg tggcaacctc
      781 gaccctgctt tcggcgcgaa tccgctcggc cgcagccgcg agcggctcgg gccgccgcgc
      841 caccaacagc acgtcattgc cggcgccggc gaagcgccgt gccagcgcca aaccgatgcc
      901 ctccgagccg ccggttacca ccgtgaacgg gcgcaggccg gcgtaggcgg cgagcgccgc
      961 gggatcggcc cgccagtagc ggcgccgcca ggcctcgatg agctttgcca tgaccgccat
     1021 gctggatcgg ggtgaccggc tcagctggcc agcgccgcca ccggcatggt tttgcgccgc
     1081 tccggcgcat tggtgcggcg aaagcgcaaa tcgaactccg cacgaatgtg gcgggcgagc
     1141 tccacggcga tctcgctttg acttgccgcc ggcaaataca tgttgatgag aaagcgcggc
     1201 agccgcggca ggcgcggatc gtccaccatc cgcaggtagt cgggaatggt ccggcgcaga
     1261 aacggcccga cggcaacgtc ggcatcaagg gaggccttga gcgcatccat gccgctctcg
     1321 cacaccggcc gccagtcgcg cttgccgtcg cgcaaggcct tgaggaccga gggacgaaac
     1381 tcgcacgttt cccgcccaag cgagatcggc aagggatcgc gacggtgtgc gacgccattg
     1441 ctggcacccg cccacaccaa gtcgtcttcg agcaaggtct ccccgtgcgg acgacaggcc
     1501 tgctcggttg tcagcgtgag atcgatgtcg cccttgcgca atgcctccag cagttgcggc
     1561 gtggtgctgc acttgagcga cacccgcaca cgcggccagg ccttgtcgaa acgcttcagg
     1621 atcggtggaa tgaaaacgcc aacgatatcg gtcggcacgc cgagccaaac ctcgccttcg
     1681 taggcgggcg cgctcatggc accccagatc tcgtcattgt gggcgatcag gcggtgggca
     1741 tgcacgatca ggcgctcacc gtcgggccgc aagctaaggc gcttgtggtg acgctcgaag
     1801 agctgcgtgc cgaacaactc ttccagccgc ttcaattgtt ggctgaccgc ggcctgcgtc
     1861 aggttgagga gggccgctgc ccccgtgacg ctgccggttt cgaccacggc cacgaaggcg
     1921 cgcagcaggg cgatgtcgat gtcgcggcgc atggggtcac cttaataagg gagcatgatg
     1981 caggatataa ataacattcg ttttatttat gcaagcgcaa agcttaggtt gagcttcaac
     2041 accgcgtggc ggtggccagg atggagaaaa ccatggcaca cctagccttc cccttagcgg
     2101 ccgcgagcgg cgccgctgcc caattcggcg gctggctgtc tcgggccttg ctcagtttcg
     2161 agttggcgct gcaggtccgc aaagagcgcc gcgcgctgca acacctgggc gacgccgggc
     2221 tcaaggatat tgggctcagc cgggccgacg tcgccgcgga gtggcaccgt tccttttggg
     2281 acgtgccggt cgatcggctg cgttgaacgg aggcttttgc tgcgaggtgg cgccggccag
     2341 cgccacctca acccttgacg cagacgatgc ctctcagccg atgcacgatt tccaccagat
     2401 cgtcttgcgc ggccatcacc gcatcgatgt ccttgtaggc catgggcgtt tcatcgatca
     2461 cgcctgcatc cttgcgacac tccacacccg ccgtcgccgc ggcgtgctcg ttgagcgaaa
     2521 atgtgcgttt ggcctccccg cgtgacatgc gacggccggc gccgtgcgcg caggactgaa
     2581 agctttccgg gtggcccttg ccgcgcacga tataggattt cgcgcccatg gaaccgggga
     2641 tgatgccgag atcgccgcgc ccggcacgca ccgcgccctt gcgggtgacc cacacggttt
     2701 cgccaaaatg ctgctcgcgc gccacatagt tatgatgaca gttgatggcg tggcgctcgg
     2761 tgcgaaaggg cggcagctgt gcacgcatga cgccgaggac ggcggccatc atgatctcac
     2821 gattgcgccg tgcaaactcc tgcgcccagg caatggcggc aacgtaatcc tcaaacaggg
     2881 gctcgccttc ctccagccag gcgagatcgc ggtcgggcag cggccgcgtc aactggcgcc
     2941 gatccagggc ttcgcgggct ttgctgatga aatagctgcc gatgcggttg ccgatgccgc
     3001 gtgagcccga atgcagcatg gcccaaacgc ggtcgctttc atcgaggcag atttcgatga
     3061 agtggttgcc gccgcccagc gagcccagat gcgtcaggcc ctgggcctga cggagcgcgg
     3121 gatgcttttc ctccagctcc tgcaaaccat cctgtagccc gctcgtgctg aagaggcgcg
     3181 ccacccgcgc gggcacgccg tccttccaac cgcccttcag cccaacgccc ccgtgtggca
     3241 ccgcggcctc gatgccaagc cgcagagccg ccaggctgtc cggcagctgc gccgccgtca
     3301 gcgaactgcg cactgccatc atgccgcagc cgatatcgac cccgacggcg gcgggcacga
     3361 tcgcggcccg gctggcgatc accgagccga ccgttgcacc tttgccgaga tgcacatcgg
     3421 gcatggccgc cacgtggtga tggatgaagg gtaacgccgc gatattgaag agctggcgcc
     3481 gcgcggcatc ctcgatcggc acgccatcga tccaggcctt gatcggcgca tagcctgtca
     3541 tgatcgtgtg tgggccgggc atggcgacaa cctcggtcgg aaaagccaac gacaagcgcc
     3601 tcaaccagcc cgcgccagct tggcactcgg tcgtgccgcc agcgataccc cggccaggat
     3661 cagcaggaaa cccacgacat gaaatgcgta gagcctttcg ccaagcaagc tggtggcgaa
     3721 tacggcggcg aacaggggga tggtgtgcag gaaggcgctg gcgcgcggcg cgccgatcaa
     3781 ctcaacgccg cgattccagg cgagataggc caaaagcgag gtgacgaggg ctgcatagac
     3841 gatggcgaag atggtgagcg gctcgaaggc gaggcgccag ccgacggcga actcccacgc
     3901 cgcaaagggc acgatgaata gggcggaggc caaggcaaac acgaacagga agctcaaggc
     3961 gtgcacgttg gggcgtaacc gaaggcaggc acaataaacc gcccagaaga ccatgttgat
     4021 gatgatcaac aggtcgccga agttgaagtt caaagccaat agccgctgca ggctcagctg
     4081 cgagacgatg gccagcacgc ccaacagcga cacgcccacg ccgaaaagct gcagcccacc
     4141 caagcgatcg ccgaacatga gccagctgcc ggcgacgatg aagaccggcg agatcgagcc
     4201 cacgacgccc atgttgagcg cggtggtgta ctgcagcgtc acgtactgca tggtgccgaa
     4261 gacactccca cccatcagcg ccaaaaacag catcagcccc aggctgcgcc gcaaggctgg
     4321 taggtccctg accacgtggc gcccggcaaa cacgccgatc aacagggcca ccaacagcca
     4381 gcgcagaa
//
LOCUS       DASTMT010000350         2804 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_192397, whole genome shotgun sequence.
ACCESSION   DASTMT010000350 DASTMT010000000
VERSION     DASTMT010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2804)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2804)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2804
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_192397"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..840
                     /locus_tag="VFR00_08215"
     CDS             <1..840
                     /locus_tag="VFR00_08215"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015688154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEU0159284.1"
                     /translation="APALIDKMMRSESLPMLGGEIRTVSLYRSDLAGFSGISEQAGPA
                     ELVTLMNEYLSAMTDIIGAHGGFVDKYVGDGIDGVFGAPLEDPRHALNAVKAALAGQA
                     RLRELNCVTPTLFLGHRLQQRIGLHTGAALVGNIGSRQRFNYTVMGDAANLASRLEGA
                     NRVYGTSIIASAETVKTAGPDVVWRELDTIRVVGRQEPVVIFEPLGLRGQETAWQKDC
                     AARYALGLTRWRSADLAGAAEAFATFAGEDPPSRRFCDRIERHLRSGRAHDWSPVHKL
                     ASK"
     gene            1845..>2804
                     /locus_tag="VFR00_08220"
     CDS             1845..>2804
                     /locus_tag="VFR00_08220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0159285.1"
                     /translation="MADACVSSKGVLGALCAGLLLLGGIPGAVAQRAQFVAPPRTIAD
                     ITAILDQEKPDPQKRARLEAEANAEPAPADTAKLRDFYYRRAQARALVGRLRDAIADS
                     EKAVAKSADYAAEGSRIELFQEAQMRLNGDYKQAIALLERMAQRLNVGQPPNRGRAFT
                     INTRTVINFIQLGEINKAESYVKRNVALLAEARSWPPAQPYLSNFEANTEDGRGRLFL
                     TRGQYKEAEAAYNKAEAGYRDALAKSRSWPNPVPAGTFEGAIDYATLFGGRAKAMQGR
                     HAEAEIDMRRALLSRLKLVGKYHADTANMLAFLSELVFEQARFR"
BASE COUNT          491 a          899 c          934 g          480 t
ORIGIN      
        1 gcgcccgcgc tgatcgacaa aatgatgcgt tcggagagcc tgccgatgct cggcggcgaa
       61 atccgtaccg tctctctcta ccgctcggac cttgcgggtt tttccggcat atccgaacag
      121 gcaggccccg ctgaactcgt gacgctgatg aacgaatatc tctccgccat gacggacatc
      181 atcggcgcgc acggcggctt tgtagacaag tacgtcggcg atggcatcga tggggtcttc
      241 ggcgcccccc tggaggatcc gcgtcatgcc ctcaatgcgg tcaaggcggc gcttgccggg
      301 caagcacgcc tgcgggagtt gaattgcgtc acgccgaccc tgtttctggg acaccggctg
      361 caacaacgca tcggcctgca caccggcgcc gccttggtcg gcaatatcgg ctcacgccag
      421 cgcttcaact ataccgtcat gggcgatgcg gccaatctcg cctcccggct cgaaggcgcc
      481 aacagggtct acggcacctc gatcatcgcc tccgcggaaa cggtgaagac ggcgggtccg
      541 gacgtggtgt ggcgcgaact cgacaccatc cgcgtcgtgg gccggcagga gcccgtggtg
      601 atcttcgagc cgctgggcct gcgcggccag gaaacagctt ggcagaagga ttgcgccgca
      661 cgctacgcct tgggcttaac gcgctggcgt tcggccgatc tggcgggcgc ggccgaggca
      721 tttgccacct ttgccggtga agatccaccg tcccgccgct tttgcgaccg catcgagcgc
      781 catctgcggt cgggtcgggc tcacgactgg tcgccggtgc acaagctcgc cagcaaatag
      841 caagctcgat ccttcctcca gcactccact ctattcgaga aaagagggct cgcttggctt
      901 tggcgcgctg gggcgaacgc tccgcgatgg caacacgcgg tcgagcagga gggggtgtcg
      961 tgcgcgcacc ccagggccct ggcgctgcga ggccgcggct tgcgggttcg agccaggcct
     1021 ttggccctgc ggctgcctca gcggccagcc caggcgcggg ggcagtttga tggggcgccg
     1081 gggggatact tttccctggc gccggacggt tgcgctcgac gggcatcggg cggccgcagc
     1141 gctgcagcag gcgcgggctg ccctcaaggc ggaagcggga catgtgcgaa acgcgtgttc
     1201 cctagggaac agcgcgcttg ggcgtagtcg agcagcatgg ccgctatgga cccctcttgc
     1261 aggcctcgcc cgaaagtcca ggagcacgac atgaccagca acccggcctt ggttctccag
     1321 cgtcaccgcg agctctccca aagccttgcc ctcgttgccc ccctcggtgc aggcgccgtc
     1381 atgagcttac acgccctaga gaacgggtcg cgcgcgccct tcgctgaggg cggctccccg
     1441 gcagcgtcgg tgggcctggg ccctgttggg gaggacccca ccccttccgg tcggcgctcg
     1501 ggcggcggac gcggctggac gattggcggt ttgctgcgaa cctgagcggc gcgccgcaac
     1561 ggccaaccgc tgccactttg gcgggggctt gccgtcagaa gccgtttcat caccgggtgc
     1621 cccgaaatct gggcggtcag gtcggtggcc gaacattgcg gttgcacagg ggacggggct
     1681 ggcaccgcgg ttgaacttca attccgtccc accacgccgg cgggccggaa gcgctgcggc
     1741 cgcagtgcca gaacctacgc ttgcgggcac ggattgcttg gtgagcttca gaaagcgcgc
     1801 taatcttttg ttctcaaacg cctttccgtg cgggagaatt gggcgtggcc gacgcgtgcg
     1861 tttccagcaa gggcgtactg ggcgctcttt gtgcgggctt gttgctcttg ggtggcatac
     1921 ccggggccgt cgcccagcgc gcgcaatttg tagcgccgcc gcgcacaatc gccgacatca
     1981 cggcaattct cgatcaggag aagcccgatc cgcaaaagcg ggcgaggctg gaagccgagg
     2041 caaacgccga gccggcgcct gctgacacgg caaagctgag ggatttctac tatcggcgcg
     2101 cgcaagcacg cgccctggtc gggcgtttgc gggacgccat tgccgatagc gagaaggccg
     2161 tcgcgaagtc cgccgattac gcggcggagg gaagccgcat cgagctgttt caagaagcgc
     2221 aaatgcggct caacggcgac tacaagcaag ccatcgcgct actggaaagg atggcgcaaa
     2281 gattgaatgt cggccagccg cccaataggg ggcgggcgtt cacgatcaac acgcgcaccg
     2341 tcatcaactt tatccagctc ggcgagatca acaaggccga gagttacgtc aaacgcaacg
     2401 tggcgctttt ggcggaggcg cgctcgtggc cgccggcgca gccctacctc tcgaacttcg
     2461 aagccaatac cgaggacggc agaggtcgcc tgtttctcac gcgtggccaa tacaaagagg
     2521 cagaggccgc ctacaataaa gccgaagcgg ggtatcggga tgcgctcgca aaatctcgat
     2581 cgtggcccaa ccccgtgccg gcgggcacct ttgagggcgc cattgattac gccacgctgt
     2641 tcggcggtcg agcaaaggca atgcaaggcc gacacgccga ggccgaaatt gatatgcgtc
     2701 gcgcactgct cagtcgtttg aagctggtcg gcaagtatca cgcggacacc gccaacatgc
     2761 ttgcctttct gtccgagctg gtcttcgaac aggcgcggtt tagg
//
LOCUS       DASTMT010000351         4479 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_524609, whole genome shotgun sequence.
ACCESSION   DASTMT010000351 DASTMT010000000
VERSION     DASTMT010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4479)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4479)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4479
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_524609"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(248..571)
                     /locus_tag="VFR00_08225"
     CDS             complement(248..571)
                     /locus_tag="VFR00_08225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020095790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6166 domain-containing protein"
                     /protein_id="HEU0159286.1"
                     /translation="MSRFEGKRTIDGLVVTVDGRRLDEHYEAKRFTSWGFEWTYEGES
                     PQQLALAILMEHLGDKERAIRLSDPFMRKVVANLDNDWELSAADIDRAIAEIEAPGRG
                     SRHDP"
     gene            747..1556
                     /locus_tag="VFR00_08230"
     CDS             747..1556
                     /locus_tag="VFR00_08230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0159287.1"
                     /translation="MAARARHLPFHDDDFLRGERIGPPLGGYRGRMLLKGAILLLAAG
                     AAGWATFSDQAWRQWVSPEMRTQLSRLIERVGAHLQTQAQPAPRIAAPPKRSEPPMRP
                     AALEAPPLAPLPTASNPAPMPDYAASKAEPPTAAEPLTTGSLPASAGAPGQPLPAPTV
                     DPANPYQVRAAAVGLHPDLSSVLLARLTATDYRNAAYAIETAVKETADSAVFVWPRQR
                     KPELALFRVRFVAGAALGCRRYVVTVVKDGWSTTAMPMEKCKSEQVSARRR"
     gene            complement(1652..2875)
                     /locus_tag="VFR00_08235"
     CDS             complement(1652..2875)
                     /locus_tag="VFR00_08235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004124429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0159288.1"
                     /translation="MRQSYGWVVVGAGMLMTCVGMGAMLSLGVFLPPLAAETGWSRTG
                     ISTAATLNFLFMGGAALVWGVLSDRFGTRIVVLLGSAILGLGLVAASRAESLIQFQLV
                     FGGLVGIAAGSFYAPMMAAATSWLEHRRNLAAALVSAGMGMGSMTMSPLAAWLIGAFD
                     WRTAMFAMGCLAWILLLPVSLLVRRPPQSAQASAVDAATGSQPYPLSSTQAVLTPQFA
                     AIALAHFACCAAHSGPIFHMVSYATLCGLPAMLAVSVFGVAGLAGLGGRIGLGIAADR
                     LGVKPVLVAGLLVQALAVGAYVFVSELKEFYALSVIFGLAYGGVMPLYAVLVRDFFGP
                     RIMGTLFGAVSMFASVGMALGPWAGGYVFDAFASYAWLYLGSFGIGLAAVAIAQSFRP
                     GRAPPRQDELALRPA"
     gene            complement(3037..3360)
                     /locus_tag="VFR00_08240"
     CDS             complement(3037..3360)
                     /locus_tag="VFR00_08240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0159289.1"
                     /translation="MHNAYVASDLLPALAFWLVALLAALPAFSIMRQGRRGRARSAIA
                     YLAGFFFGLALAVSLSFAAAALIAADYPLFNAGVTAAFLGPFTGLVHAKLRRAPRRRT
                     RLAEA"
     gene            3507..4340
                     /locus_tag="VFR00_08245"
     CDS             3507..4340
                     /locus_tag="VFR00_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HEU0159290.1"
                     /translation="MSVDFSTLIWIFLALMALQPLLTGRWFAFHRLKAIQSIEKARGS
                     RVITMIHRQEKRSLFGFNVARHIDLEDAQTIIAAIKATPAERPIDLILHTPGGLVLAA
                     MQIARAVEAHAGKVTVFVPVYAMSGGTLIALAADEIVLGEFSVLGPIDPQIAGFPAAS
                     IVRVRQSKPVEHIFDLTLVLADMSEKALAQVKRGAVELLTPRLDQAAAEKLAEKLAGG
                     HWTHDYALTAEEARALGLPVTVGMPSEVLDLMKLYPQPVQRSGVDFLPIDVPAKRAMG
                     S"
BASE COUNT          817 a         1574 c         1428 g          660 t
ORIGIN      
        1 gcctcgccgg cacgggcggc accggttttt tgctgagcag cacagtggtc atgggatcgc
       61 tttaatcctc agctcgctgg ccaatactgg cgccgccgtc ctgagcgcgg cgaccgacgc
      121 gacgccggta gcacatgccg ccctgcctcc gcctcccccg cggcccggcg tgcggcgatg
      181 atcgtgacta tgaacgtgac cctgagcgtg actatgagcg cgacaatgaa cgtcacaatg
      241 aacgtcacta gggatcgtgc ctcgagcctc gacctggcgc ctcgatttcg gcaatggcgc
      301 ggtcgatatc ggccgccgac aactcccagt cgttatcgag attggcgacg accttgcgca
      361 tgaagggatc gctgaggcgg atggcgcgct ccttgtcgcc cagatgttcc ataagaatgg
      421 caagcgccag ctgctgcggg ctctcgccct cgtaggtcca ttcgaacccc caggacgtga
      481 agcgcttggc ctcgtaatgc tcatcgagcc gtcggccatc gacggtgacg accaagccat
      541 cgatggtgcg cttgccttca aacctgctca cgggctccct cctccgccca acgtgggtga
      601 tatatcaccc ttcgaatggc cggcgatagc cccgcttggc ggcgatttgc atcgtgttat
      661 cgccggcctt gccctcattt cgccaggaat ttgctggcgc ctatacaacc gcgggaaccc
      721 aggacgacag acagcaggga tcaatcatgg cagcccgcgc ccgtcacttg cccttccacg
      781 acgacgactt cctgcggggc gagcgcattg gcccgccgct gggcggttac cgcggcagaa
      841 tgctgctcaa aggcgccatc ttgctgctcg cggccggcgc cgccggttgg gccaccttca
      901 gcgaccaggc ctggcgccag tgggtgtcgc cggagatgag gacccagctg tcgcgcctga
      961 tcgagcgcgt cggcgcccac ctgcagacgc aagcgcaacc ggccccgcgc atcgccgctc
     1021 cacccaagcg cagcgaaccg cccatgagac ctgctgccct ggaagccccg cccttggcgc
     1081 cactgcccac cgccagcaac ccagcgccga tgcccgatta cgccgccagc aaggccgagc
     1141 cgcctacagc cgccgaaccc ctgacgacgg gctccctgcc cgcgtcggcc ggcgcaccgg
     1201 gacagccgct tcctgcgccc acggtcgatc ccgccaatcc ctaccaggtg cgcgccgccg
     1261 ccgtcggcct gcaccctgat ctttcctccg tcctgctggc gcgcctgacg gccacggact
     1321 atcgcaatgc cgcctatgcc atcgaaaccg cggtcaagga aaccgccgat tccgccgtct
     1381 tcgtctggcc acgtcaacgc aagcccgagc tggcgctgtt tcgggtgcgc tttgtcgccg
     1441 gcgccgcgct gggctgccgg cgctacgtcg tgaccgtcgt caaggacggc tggtcgacga
     1501 cggcaatgcc gatggaaaag tgcaaatccg agcaggtctc ggcccggcgc cgctgagtta
     1561 acggccgcca cccgtcccac cttgttggcg gcgccagccc ttcaagcgcg ccggcccttc
     1621 aagcggccct gactttcagg cgggcctgac gctaagcggg tctgagcgcg agctcgtcct
     1681 gacgcggtgg cgctcggccc ggccgaaagc tctgggcaat ggccaccgcc gccagcccaa
     1741 tgccaaacga acccaggtag agccaggcgt agctggcaaa ggcgtcgaac acataaccgc
     1801 ccgcccaggg gccgagcgcc atgccgacgc ttgcgaacat ggagaccgcc ccgaacaggg
     1861 tccccatgat gcgcgggccg aaaaagtcgc gcaccagcac ggcataaagc ggcatgacgc
     1921 cgccgtaggc caagccgaag atgaccgaca gagcgtagaa ttccttcagc tcactgacaa
     1981 agacataggc acccaccgcc agcgcctgca ccaacagacc cgcgaccagc acgggtttga
     2041 ccccaagccg atcggcggca atgccgagcc cgatgcggcc gccgaggccg gcgaggccgg
     2101 ccacgccgaa gacgctgacg gcgagcatcg ccggcagacc gcagagcgtg gcgtaggaga
     2161 ccatgtgaaa aatgggcccc gaatgcgcgg cgcaacaggc aaaatgcgca agcgcaatgg
     2221 ccgcgaactg cggtgtgagc acggcttgcg tgctgctcag gggatagggt tgcgagcccg
     2281 tcgcggcgtc caccgcagag gcctgcgcgc tctggggcgg ccgccgcacc agcaacgaca
     2341 cgggcagcag caggatccag gccaggcaac ccatggcaaa catggccgtg cgccagtcga
     2401 aggcgccgat cagccaggcc gccagcggcg acatcgtcat cgagcccatg cccatgcccg
     2461 ccgacaccag ggcggcggcg agattgcggc ggtgctcgag ccagctggtg gccgcggcca
     2521 tcatcggcgc ataaaaactg cccgcggcga tgccaaccaa gccgccgaac accagctgaa
     2581 actgaatgag gctttcggca cggctggccg cgaccagacc aaggccgaga atggcgctgc
     2641 ccaatagcac gacgatgcgc gtgccaaagc ggtccgatag cacgccccag acgagggcgg
     2701 ccccgcccat gaacaggaag ttaagcgttg cggccgtcga aatacccgtt cgcgaccagc
     2761 cggtctcagc cgccagcggc ggcagaaaga ccccgagcga gagcatggct cccatgccga
     2821 cgcaggtcat cagcatcccc gcgccgacaa cgacccaacc gtatgactgc ctcatgctcc
     2881 tcccggcggc gcgccatccc gctgcgttgc cattgctggg cctcccctgg gatcgccgcg
     2941 gcgaccgcca gcctcgattg cgagcaagac ccgtgcggtg ccaccctaac ggagacgccg
     3001 gcccctggga atgctttgcc atgccgggcg cgcgggtcag gcttcggcca accgcgtgcg
     3061 ccgacgtggc gcgcggcgca gctttgcatg cacaaggccg gtaaaggggc ccaagaaggc
     3121 cgccgtcacc ccggcattga agagggggta gtccgcggca atcagcgccg cggcggcaaa
     3181 cgagaggcta accgccaggg ccagcccgaa aaagaagccg gcgagatagg cgatggcact
     3241 gcgcgcgcgg ccgcgccggc cttggcgcat gatgctgaag gccggcaggg cggccaggag
     3301 ggcaacgagc cagaaagcca gtgcgggcag caaatcggac gccacgtacg cgttgtgcat
     3361 gtgctcaccg tgtctgcggg ctgctttagg tgattcgctt ggcgaaatga ggccattggc
     3421 aaaaatcgcg ttgcccagcc ccatggcggt cggctaggcc tacgcctact cgctttgctt
     3481 cgcatccacg tcgccaggag ccgaccatgt ccgtcgattt ctcgacgctg atttggatct
     3541 ttctagccct catggcgctg cagccgctgc tgacgggcag atggttcgcc ttccatcgcc
     3601 tgaaggcgat ccaaagcatc gagaaggcgc gcgggagccg cgtcatcacc atgatccacc
     3661 gccaggaaaa gcgcagcctg ttcggcttca acgtcgcccg tcacatcgat ttggaagacg
     3721 cccagaccat tatcgcggcg atcaaggcga cgccggcgga gcggccgatc gatctgatct
     3781 tgcacacccc ggggggcctg gtgttggcgg cgatgcagat cgcgcgcgcc gtcgaggcgc
     3841 atgccggcaa ggtcacggtc ttcgttcccg tctacgccat gtccggcggc acgctcatcg
     3901 ccctggcggc cgacgagatc gtgctcgggg agttctcggt gttgggcccg atcgatccgc
     3961 aaattgccgg ctttcccgcc gccagcatcg tgcgcgtgcg tcaatccaaa cccgtcgagc
     4021 acatcttcga cttgacgctc gttctggccg acatgagcga aaaggccttg gcacaggtca
     4081 aacgcggggc ggtcgagttg ctcaccccgc ggctcgatca ggccgcggcc gagaagctgg
     4141 cggagaagct ggcgggcgga cactggacgc acgactatgc cctgaccgcc gaggaggcga
     4201 gggcgctggg tctgccggtc accgtcggca tgcccagcga ggttctggac ttgatgaagc
     4261 tctacccgca gcccgtgcaa cgatcgggcg tcgatttctt gcccatcgac gtgccggcaa
     4321 agcgggcgat gggatcgtga gcgcacggcc aagcgccgcg acagccccca ccgcgcgcga
     4381 gcccggcaca atgccatccc gcccattttg atctccccct aagcggggat gcagagcggt
     4441 gtggcattga ccatctcagc cgcggctccg gcgccatcg
//
LOCUS       DASTMT010000352         1592 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_1505740, whole genome shotgun sequence.
ACCESSION   DASTMT010000352 DASTMT010000000
VERSION     DASTMT010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 1592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1592
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1505740"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1592)
                     /locus_tag="VFR00_08250"
     CDS             complement(<1..>1592)
                     /locus_tag="VFR00_08250"
                     /inference="COORDINATES: protein motif:HMM:NF006192.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional aldolase/short-chain dehydrogenase"
                     /protein_id="HEU0159291.1"
                     /translation="MLSRWRDADARKAIEGYAKAGIGEDLALRTYSTRLLGGDPLLVL
                     HGGGNTSVKTRLADLVGETHEVLCVKGSGWDMASIEPAGLPAVKLEPLRQLRRLDRLA
                     DEDMVNFQRTNLLNASAPNPSVETLLHAFLPGRFIDHTHAAAVLSLVDQPNGQELVDD
                     VYDGSMGFVPYVIPGFGLAKLAAEVFEAKPGVEGLILHKHGIFTFGESAREAYERMIA
                     MVTRAEMRLAQGRRNVFAAAALPPALANLAEVAPIIRGAIAVPASAAQAEPKRFLLEF
                     RGGREVLNYVNGVDLADYGQRGVVTPDHIIRTKNYPLLAPPAPAGKLEAFQQGLRQAV
                     ADYVARYDAMFQRQNARVNGSKSKLDPMPRVVLVPGLGLFGVGTTARDAAIAADLAEN
                     AVRVITDAEATGRFEPLPEADLFDVEYWSLEQAKLKGAVAKACTGQVAVVTGGASGIG
                     AATARAFADEGAAVVVLDRAEAATTAVAKEVKGLAVTCNVCDRSSVAAAFAAIVARFG
                     GVDIVVSNAGAAWQGRIGEVSD"
BASE COUNT          267 a          556 c          509 g          260 t
ORIGIN      
        1 gtcggacacc tcgccgatcc gtccctgcca ggccgcgccg gcgttcgaga ccacaatatc
       61 cacgccgccg aacctggcca cgatggcggc gaaggcggcg gcaacggagc tgcgatcgca
      121 gacgttgcag gtaacggcca ggccctttac ctccttggcg acggccgtgg tggccgcctc
      181 ggcacggtcg agcaccacca ccgccgctcc ctcgtcggca aacgctctcg ccgtcgccgc
      241 gccgatgccg ctggcgccgc ccgtcacgac cgcgacctgg ccggtgcagg ccttagccac
      301 ggcgcccttg agcttggctt gctccaggct ccagtactcc acatcgaaca ggtccgcttc
      361 cggcaaaggt tcgaaacggc cggtggcttc ggcatcggta atgacgcgaa cggcgttctc
      421 ggcgaggtcg gcggcaatgg cggcatccct ggccgtggtg ccgacgccaa atagccccag
      481 ccccggcacc agcaccacac gcggcattgg atcgagcttg ctcttgctcc cattaacgcg
      541 tgcgttttgg cgctggaaca tggcgtcata cctcgccaca tagtcggcca ccgcctggcg
      601 caggccctgc tggaaggcct cgagcttgcc cgccggcgcc ggcggcgcca gcaggggata
      661 gtttttggtg cgaatgatat ggtcgggcgt aaccacaccg cgctggccat agtcggccaa
      721 atcgacgccg ttgacgtagt tcaacacctc ccggccgcca cggaactcga gcaggaagcg
      781 ttttggttct gcctgcgccg cgctcgcggg caccgcgatg gcaccgcgga tgatgggtgc
      841 cacctcggca agattggcca gtgccggcgg caatgctgcc gcggcgaaga cgttgcggcg
      901 accctgcgcc agccgcatct cggcgcgcgt caccatcgcg atcatgcgct cataggcctc
      961 gcgggcgctc tcgccgaacg tgaagatgcc gtgcttgtgc aggatcagcc cctcgacgcc
     1021 gggcttagcc tcgaacacct ctgccgccag cttggcaagg ccaaatcccg gaatgacgta
     1081 gggcacgaag cccatgctgc cgtcatagac gtcgtcgacc aactcctggc cgttcggctg
     1141 atcgaccagg ctcagcaccg ccgccgcatg cgtgtggtcg atgaatctgc cgggcaggaa
     1201 ggcatgcaag agggtttcga ccgatggatt gggtgcgctg gcattgagca agttcgtgcg
     1261 ctgaaagttg accatgtcct cgtcggcgag ccgatcgagc cggcgcagct ggcgcaaagg
     1321 ctcgagcttt accgccggca gccccgccgg ctcgatgctg gccatgtccc agccagagcc
     1381 cttgacgcac agaacctcgt gcgtctcgcc caccaggtcg gcaagccgag tcttgaccga
     1441 agtgttgccg ccgccgtgca ggacgaggag aggatcgccg ccaagcaagc gcgtactata
     1501 ggtgcgcaac gccaggtcct cgccgatgcc ggccttggcg tagccctcga tagccttgcg
     1561 ggcgtcggca tccctccagc ggctcaacat cg
//
LOCUS       DASTMT010000353         2219 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_3475527, whole genome shotgun sequence.
ACCESSION   DASTMT010000353 DASTMT010000000
VERSION     DASTMT010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2219)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2219)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2219
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3475527"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..390)
                     /locus_tag="VFR00_08255"
     CDS             complement(<1..390)
                     /locus_tag="VFR00_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018407738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEU0159292.1"
                     /translation="MDATSIDRRCCIVGGGPAGMMLGLLLARAGVEVDVLEKHADFLR
                     DFRGDTIHPSTLEVMYELGLLASFLERPHQKIEQLAAVIGDRSLRIADFTHLSTHCRF
                     IALMPQWDFLDFIATQAKRYANFQLRMQ"
     gene            complement(414..1205)
                     /locus_tag="VFR00_08260"
     CDS             complement(414..1205)
                     /locus_tag="VFR00_08260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020094387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU0159293.1"
                     /translation="MQTLTGKVAWVTGAGTGIGEAGAAALAEAGMTVVLSGRRAAPLE
                     AVAQRLNATAAGKARVQAADLSQKGEVKRIADVIKAELGRLDVVVNNAGCNIPERAWR
                     NLTADGIDTLIQGNLTSAFYVVAAALPIMRAQGDGLFIHTASWAGRFIGPVSGPGYTA
                     AKHGMVAMSHTINLEECMHGIRSCVVCPGEVATPILKNRPIPETAETMARMLQPDDMG
                     QLIAFIAKQPGHVCINEVLISPTYNRGYIGTMKAREAALAAAGKT"
     gene            complement(1284..>2219)
                     /locus_tag="VFR00_08265"
     CDS             complement(1284..>2219)
                     /locus_tag="VFR00_08265"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEU0159294.1"
                     /translation="GYLLPTREQVMAGQFEVRSLLDLASRAAALGFDAIWVGDSLLAR
                     PRHDPLTLLAALAARLPAVALGTAVLLPALRNPVLLAQQIATIDQIAEGRLILGVGIA
                     SDLPNIRAEFVAAGVPFEKRVGRLLEGLRLCRALWTGTRVDWQGRWTLEGGVLGPLPH
                     RPGGPPIWLAGAQPASLQRVAQLFDGWLPNSPTPARWAQQWQEIGASARAAGRDPEAL
                     VGAMYLTLAVDEDPQRAQARLDAYLAQYYGRPAAEVRARQACYAGPARGMLDWLTAYA
                     QAGAKHLVLRFAGEHERHLQTVAQIRAQLASAPAP"
BASE COUNT          377 a          765 c          719 g          358 t
ORIGIN      
        1 ctgcatgcgc aactggaaat tggcgtagcg cttcgcctgc gtggcgatga agtcaaggaa
       61 gtcccactgc ggcatcaagg caatgaagcg gcagtgcgtc gagaggtgcg tgaaatctgc
      121 gatgcgcagc gacctgtcgc cgatgaccgc ggcaagctgc tcaatttttt gatgcggtcg
      181 ctctagaaag ctggcaagca gcccgagctc gtacatcacc tcgagcgtcg agggatggat
      241 ggtgtcgccc cgaaagtcac gcagaaaatc cgcgtgtttt tcgagcacgt cgacctctac
      301 gccggcgcgc gccagcagca agcccagcat catgccggcg gggccgccgc cgacaatgca
      361 gcagcgccta tcgatgctgg ttgcgtccat gtgccgtcag gtccgcaaca gcgctaggtt
      421 ttgccagccg cagccagcgc cgcctcgcgt gccttcattg tgccgatata gccgcggttg
      481 taggtcgggc tgatgagcac ctcattgatg cacacgtgcc ccggttgttt ggcgatgaag
      541 gcgatcaatt ggcccatatc atccggctgc agcatgcgtg ccattgtctc tgccgtctcg
      601 ggaattggac ggtttttcag gataggcgtc gccacctcgc cagggcacac gacgcaggag
      661 cgaatcccgt gcatgcactc ctcgagattg atggtgtggc tcatcgccac catgccgtgc
      721 ttggccgccg tatacccggg cccggacact ggaccgatga aacggccggc ccacgatgcc
      781 gtgtggatga agaggccgtc accctgcgcg cgcatgatcg gcagcgccgc cgccacgacg
      841 taaaacgcgc tcgtcaaatt gccttgaatg agcgtgtcga taccatcagc ggtcaagttg
      901 cgccaagcgc gctcgggaat gttgcacccg gcattgttga cgacgacatc caagcggccc
      961 agttcggcct tgatcacgtc ggcgatcctc ttgacctcgc ccttctgact gaggtcggcg
     1021 gcttgcacgc gcgctttgcc ggccgcggtg gcgttgagcc gctgcgccac ggcttcgagc
     1081 ggcgccgccc gccggccgct gagtacgacc gtcattcctg cctcggccag ggctgccgca
     1141 ccggcttcgc cgataccggt gccggcgccc gtcacccatg ccaccttgcc cgtcagcgtt
     1201 tgcatgcctt ggctccttgc cgttgctcaa gccgttctag cgccaccgat accgtctcga
     1261 tagtcgcagc acccccaagc tgcctagggc gccggcgcgc tggcaagctg ggcccggatc
     1321 tgcgccaccg tctgcaggtg acgctcgtgt tcaccggcaa agcgcaacac gaggtgcttg
     1381 gcacccgcct gggcgtaggc cgtcagccaa tccagcatgc cccgggcggg gccggcatag
     1441 caggcctgcc gcgcccgcac ctcggcggcc ggtcggccgt agtactgcgc cagataggca
     1501 tcaagccgcg cttgcgcgcg ctgggggtcc tcgtcaacgg ccaaggtcag atacatcgca
     1561 ccgaccagcg cctctggatc gcggccagcg gcgcgggcgc tcgccccgat ctcctgccac
     1621 tgctgagccc aacgcgcggg tgtgggagag ttcggcagcc agccgtcgaa aagctgagcg
     1681 acacgctgca ggctcgcggg ctgggcgccc gccagccaga tcggcggccc cccgggccga
     1741 tgtggcagcg gcccaagcac acccccctct agcgtccaac gaccctgcca atcgacccgg
     1801 gtgccggtcc acagggcgcg acagagcctg aggccctcca acaggcgccc gacccgcttc
     1861 tcgaagggca cgccggcggc gacgaactcg gcccgaatat tcggcaagtc gctggcaatg
     1921 ccgaccccga ggatcaggcg gccttcggca atctggtcga tggtggcgat ctgctgcgcc
     1981 aacagcaccg gattgcgcag ggcaggcagc agcaccgcgg ttccgagcgc gaccgcgggc
     2041 aggcgtgcgg cgagcgcggc gagcaaggtc aaaggatcat gccgcgggcg cgccagcagg
     2101 gaatcgccaa cccagatggc gtcgaacccg agggctgccg cgcggctggc caggtccagc
     2161 agcgagcgca cttcgaactg ccccgccatc acctgctcgc gggtcggcag caggtagcc
//
LOCUS       DASTMT010000354         8636 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_3475531, whole genome shotgun sequence.
ACCESSION   DASTMT010000354 DASTMT010000000
VERSION     DASTMT010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 8636)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8636)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8636
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3475531"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1407)
                     /locus_tag="VFR00_08270"
     CDS             complement(<1..1407)
                     /locus_tag="VFR00_08270"
                     /inference="COORDINATES: protein motif:HMM:NF019552.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HEU0159295.1"
                     /translation="MQNATPEPATSSGLSAWLRLGANFDARTKLQVALLLLDRARAQL
                     LAYARRSELLSWSPARPHADELLMAPPNLRVPDPSFVDELKWGNFGLAGEIAELRGRS
                     PFARAPASADWARELHGFSWLRHLDAARAEDTERMARRLLKDWLQTGSRDKAHAWAPE
                     ATGRRLMSWLAHSQLLLDAADAKFHALVVRSLQRQASYLSAAWQNAPEGYPKLVALIG
                     LVLADLCIEGRDRQLKRSLKLLSTEIERQILSDGGHLGRNPQTLVELVLDLLPVRQCL
                     LVRGLAVADPLARALDAMVPMLRHLRLGEGSLARFNGVGPTDRDALATVLAYDKRATA
                     LPALAAPSGYARLERGGSIVVVDVGGPPPLSVGHAAHAGCLSFEMSVGGEPLFVNNGA
                     PASRDPARRALARATASHNTLCLSEQSSSKLSRRGAAGGAMPIEQPERVSASLAEDAG
                     GLRLEASHDGYVERWGLLH"
     gene            complement(1482..2771)
                     /locus_tag="VFR00_08275"
     CDS             complement(1482..2771)
                     /locus_tag="VFR00_08275"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HEU0159296.1"
                     /translation="MAARRLAVHLVAGVLRHRRPLEQVLGDASRQPDLFLLPARDRGL
                     ARLVAATVLRRQGELEQVLNAFIERPLPADQGQLWPILLCAAAQLLCLALPAHAVVDL
                     AVELVRRDKGAHRFAKLSNAVLRRLSERGPGLLAAQDGVRLNIPSWLWQRWGDAYGPD
                     TARQIGEASLREAALDLSLKPREDVGAWADRLGGQPLPTGSIRLASPGRIEELPGYAE
                     GAWWVQDAAAALVVRLAGDVAGLSVADLCAAPGGKTAQLAAAGAVVTAVDISLQRLER
                     LKANLDRLQLSASLVSADVASWAPGTAFDAVVLDAPCTATGTIRRHPDILRLKAADDV
                     ARMARLQQSLLANAARLLRPQGLLIYSTCSLEPEEGPEQIAQFLASHAGFERAPVTAD
                     ALGIPAESVTPHGELRTLPVHAEGMDGFYVAALRRLS"
     gene            complement(2853..3794)
                     /gene="htpX"
                     /locus_tag="VFR00_08280"
     CDS             complement(2853..3794)
                     /gene="htpX"
                     /locus_tag="VFR00_08280"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011501199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HEU0159297.1"
                     /translation="MNFARTGLLLAALTGIFVAMGALLGGTSGMVIAFVMALGMNAFS
                     LWQSDKMVLRLYGAEEVDANSGGEYYAIVADLVRKAGLPMPRVYIMHNPQPNAFATGR
                     NPANAAVCASTGLIEMLSADEVAGVMAHELAHVKNYDTLTMAVAATIGGAVSMLAQYL
                     QFGALFGGNRQNNSVGWLGTLAAIILAPLAAMLVQMAISRSREYQADRLGAMICGAPL
                     SLASALDKIEQYAHDIRNEPAEAAPATAHLFIINPLTGEGMDNLFSTHPNTGNRIAEL
                     ERLAEEMGATGAAGRGASSSDLRGAPPATGRRGGPWG"
     gene            4070..4276
                     /locus_tag="VFR00_08285"
     CDS             4070..4276
                     /locus_tag="VFR00_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007010908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1674 domain-containing protein"
                     /protein_id="HEU0159298.1"
                     /translation="MCQDRDRSAPAPPKGLTPQAERALREAEERRLRAQQGAAPQPKE
                     IGGRQGPDPTRYGDWEKNGIASDF"
     gene            4442..5170
                     /locus_tag="VFR00_08290"
     CDS             4442..5170
                     /locus_tag="VFR00_08290"
                     /inference="COORDINATES: protein motif:HMM:NF018597.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extensin family protein"
                     /protein_id="HEU0159299.1"
                     /translation="MPISPEPKPRHRLIVRLLLLVLLGLGAGFLFRQGMIPAVVNPLP
                     ALDLSQADPWFVDWRLAALRHDPGLCRRMLAQPEIRAEPIADNPPKDGCGWTNSVRMT
                     AAGGAHAAFDKATCEAATALALWLTHGVQPLAQELLGQRVTSIQTFGSYACRNIVGNP
                     FWKDIRSEHATANALDISGFTLAGGRQISVAQHWKGTGPEARFLRAVHARACHYFHVV
                     LGPDYNAAHHNHFHLDRGLFWRCT"
     gene            complement(5174..5860)
                     /locus_tag="VFR00_08295"
     CDS             complement(5174..5860)
                     /locus_tag="VFR00_08295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020184895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04282 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HEU0159300.1"
                     /translation="MAQRRRRASLPGAPPRAAPFRCRLVVMAKAPLAGAAKRRLAAEI
                     GSTEAIRFARAAISALMSRVGRDRRWQTILAVSPDRFADSPIWPAKSKICAQGPGDLG
                     QRMQRLCQATAPGPLVIVGTDIPNLTAQHIARAFRLLGNRDAVFGPALDGGYWLVGLR
                     RRPRLPRPFRAVRWSSPHALSDTLANLSDHRIGFLGTLGDVDSAEDWRRSAPGAGRRL
                     LPGPLRFGAR"
     gene            complement(5889..6848)
                     /locus_tag="VFR00_08300"
     CDS             complement(5889..6848)
                     /locus_tag="VFR00_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008972119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU0159301.1"
                     /translation="MQSISRRGFVISAATAGAVFGLDGPLEFVGPAFAQKPDPKLMDK
                     GVIKFKVGDIEVIQMYDGLWEKAHDPNFVKNASLDEVKAALKAGGLTEEFVPISFTVT
                     AVKSKGKLVLFDAGTGGQVSPKAGLVAAKNMKAAGIDPDKVSTIVVTHFHGDHISGLM
                     AKDTNAPIFPNAEILVPAAEYKFWTDPSTTAGAAKRIQAVFPGWKNIKQFEGDKEIAP
                     GVRPIASNGHTPGHTSYQLASGKKQLIVLGDVTNIPALNLKNPGWHLVFDADPVLAET
                     NRRKMFDRVIADKVTVTGYHFGFPGAGSINKDGKGYTFVPVKA"
     gene            complement(6951..7640)
                     /locus_tag="VFR00_08305"
     CDS             complement(6951..7640)
                     /locus_tag="VFR00_08305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012043494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04283 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HEU0159302.1"
                     /translation="MISVVIPTLDSEASLPATLASLVTAAVDGLVREVIVVDGGSRDA
                     TAAIADSSGARVLVGPRGRGSQLALGAKEARFPWLLFLHADTRLGDGWEREALAFLRR
                     VDSEDSPLGAAAFRFALDDQGLRPRLLERLVALRCMLLRLPYGDQGLLIPKRLYQTLG
                     GYRPLLLMEDVDLVRRLGRRRVSMLRAPAITSAERFVREGYGRRSARNLLCLALYLLR
                     VPIGTIHRIYG"
     gene            7835..>8636
                     /locus_tag="VFR00_08310"
     CDS             7835..>8636
                     /locus_tag="VFR00_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011665807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PA0069 family radical SAM protein"
                     /protein_id="HEU0159303.1"
                     /translation="MGAPAPAHSPEAAEGEAALVEAERRRGRGARSNRVGRFEAELRE
                     SFDDGWEGLGDLAPFKTQVFRETAKSIIATNDSPDISFDQSINPYRGCEHGCIYCFAR
                     PTHCYLGHSAGLDFETKLYAKINGADLLERELAHSRYRAKVIALGAVTDPYQPIEREH
                     RLTRALLEVLDRTSHPVGIVTKSALVVRDIDLLARMAKRDLVKVAISVTTLDRQIARK
                     MEPRAATPGKRLEAIAALAAAGVPVAVMVAPIVPALNDSEIESILSAAQ"
BASE COUNT         1473 a         2940 c         2887 g         1336 t
ORIGIN      
        1 gtgcaagagc ccccagcgct cgacatagcc gtcatggctg gcctcaagcc gcaatccccc
       61 ggcgtcctcg gcgaggcttg cgctgacgcg ctcgggctgc tcgatcggca tggcaccgcc
      121 cgcggcgccg cggcggctca gcttggagga ggactgctcg ctgagacaaa gcgtattgtg
      181 gctcgcggtt gcgcgcgcca gcgcgcggcg cgccgggtcc cgcgaggcgg gcgcgccatt
      241 gttgacgaac agcggttcgc cgccgacgct catttcgaac gacaggcagc cggcgtgcgc
      301 tgcgtgcccc acgctgagcg gcggcgggcc gcccacgtcg acaaccacta ttgagccacc
      361 ccgctcaagg cgcgcgtagc ccgacggcgc ggccaaggcc ggcaaggccg tggcgcgctt
      421 gtcataggcc aacaccgtcg ccaaggcatc ccgatcggtc ggccccacgc cattgaagcg
      481 ggcgaggctg ccctcgccca agcgcaaatg ccgtagcatc ggcaccatgg cgtcgagcgc
      541 gcgcgccaag gggtcggcga cggccaagcc gcgcaccagc aagcactgcc gcacgggaag
      601 caaatcgagc accagttcga cgagcgtctg cggattgcgg ccgaggtggc cgccgtcgct
      661 gaggatctga cgctcgattt ccgtgctcag cagttttagc gagcgcttga gctgccgatc
      721 gcggccctca atgcacagat cggcaagcac cagcccgatc agggccacga gcttggggta
      781 accctccggc gcgttctgcc aggcagccga cagatagctc gcttgccgct gcaggctgcg
      841 cacgacgagc gcatggaatt tggcgtcggc tgcgtcgagc aggagttggg aatgcgccaa
      901 ccaggacatc aggcggcgac ccgtcgcctc cggcgcccag gcatgagcct tgtctcggct
      961 acccgtctgc aaccagtcct tgagtaaccg gcgcgccatg cgctcggtat cctccgcccg
     1021 tgccgcatcg aggtggcgca accaggaaaa gccgtgcagc tcgcgggccc agtccgcact
     1081 ggcaggcgca cgcgcaaagg gcgagcgccc ccgaagttcg gcgatctcgc cggccaaacc
     1141 aaagttgccc catttgagct cgtcgacaaa acttggatcc ggcacacgca ggtttggcgg
     1201 cgccatcagc agctcgtccg cgtgcggccg cgccggggac caggacagga gttcggagcg
     1261 gcgcgcatag gccagcagct gggcgcgcgc ccgatcgagg agaaggaggg caacctggag
     1321 cttggtgcgc gcgtcgaaat tggcgcccaa ccgcaaccat gcggagagac cagaggaggt
     1381 cgccggctcc ggggtggcat tctgcatcga gctcccgtta actcggccgc caacgccagg
     1441 caagaccgag cccccgcaaa gtctgtgggt gagcggtgag ctcagctcag ccgccgcagc
     1501 gccgccacat aaaagccgtc catgccttcg gcatggacgg gcagcgtgcg cagctcaccg
     1561 tgcggcgtca cgctttcggc tggaatgccg agcgcatcgg ctgtcaccgg cgcgcgctcg
     1621 aagccggcat ggctggcaag gaactgggca atctgctcgg ggccttcctc cggctcgagc
     1681 gagcaggtgc tgtagatcaa gagcccctgc ggccgcagca ggcgggcggc gttggcgagc
     1741 aggctctgct gcaaccgcgc catgcgggca acatcgtcgg ccgccttcaa acgcaggatg
     1801 tcggggtggc gacggatggt tcccgtcgcc gtacaggggg catcgagcac gacggcgtcg
     1861 aaggccgtac ccggcgccca gctggcaacg tcggcgctga cgaggctcgc cgacagctgc
     1921 aggcgatcga ggttggcttt tagccgttcg agccgctgga gcgaaatatc gacggcggtc
     1981 accacggcgc ccgcggcggc gagctgagcc gttttgccgc ccggtgccgc gcataaatcg
     2041 gccacggaca agcccgccac gtcgccggcc agtcgcacaa ccagagcggc ggcggcatcc
     2101 tggacccacc aggcgccttc ggcatagccc ggcagctcct cgatgcgacc aggcgaggcg
     2161 aggcggatcg agcccgtcgg cagcggctgg ccaccgaggc gatcggccca ggcgcccaca
     2221 tcttcccgag gcttcaggct gaggtcgagg gcggcttcgc gcagcgaggc ctcgccgatc
     2281 tggcgcgccg tgtcgggccc gtaagcgtcg ccccagcgtt gccagagcca acttgggatg
     2341 ttgagccgca caccgtcttg ggcggcgagc agcccggggc cgcgctcgct caagcgccgc
     2401 agcacggcat tgctcagttt ggcgaagcga tgtgcgccct tatcgcggcg cacgagttcg
     2461 accgcgaggt cgacaaccgc gtgggcgggg agcgcgaggc aaagaagctg tgcggcggca
     2521 cacaacagga tcggccacaa ctggccctgg tcggcgggca gtggccgttc gataaaggcg
     2581 ttcagcacct gttcgagctc gccctgccgg cgcagaaccg tggcggcgac caaccgcgcc
     2641 aagccgcgat cgcgcgccgg cagcagaaaa agatccggct gacgcgaggc atcgccgagc
     2701 acctgctcga gcggacgccg gtggcgcaag acgcccgcga ccaagtggac ggcgagacgg
     2761 cgcgccgcca aaccctccgg cgcaacgcgt gggcgcgcgg gcctgacggc aacggcggct
     2821 ttcgggcgat gcctgctcgg cggcaaaaac gtctagcccc agggtccccc gcgccggccg
     2881 gtcgccggcg gcgcgccccg gagatccgag ctgctggccc cgcgcccggc ggctcccgtc
     2941 gctcccatct cctcggccag tcgctccaac tcggcgatgc gattgccggt gttgggatgg
     3001 gtcgagaaca ggttgtccat gccctccccc gtgagcggat tgatgatgaa caggtgcgcc
     3061 gttgccgggg ccgcctccgc gggctcgttg cgaatgtcgt gcgcgtactg ttcgatcttg
     3121 tccaaggccg aggccagcga cagcggcgcg ccgcagatca tggcgccgag ccggtcggcc
     3181 tggtactcgc gcgaacggct gatcgccatc tgcaccagca tggcggcaag cggggcaagg
     3241 atgatggccg ccagggtccc caaccagccg acgctattgt tttgccggtt accgccgaac
     3301 agggccccga attgcagata ctgcgccaac atggaaacgg cgccgccgat ggtggcggcg
     3361 acggccatcg tcagggtgtc atagtttttt acgtgtgcga gttcgtgcgc catcacgccg
     3421 gcgacctcgt ccgccgatag catctcgatc agccccgtcg aggcgcagac ggcggcattg
     3481 gcgggattgc gcccggtggc aaaggcattg ggctgcgggt tgtgcatgat gtaaacgcgc
     3541 ggcatcggta gcccggcctt gcgcacgaga tcggcgacga tggcatagta ttcacccccg
     3601 gaattggcgt cgacctcttc ggcgccatat aggcgcaaca ccattttgtc ggattgccag
     3661 aggctaaagg cgttcatgcc gagcgccatc acgaaggcga tgaccatgcc gctggtgccg
     3721 cccaagaggg cgcccatggc gacaaaaatg cccgtcaagg ccgccagcag caggccggtc
     3781 ctggcgaaat tcatgggcat ggcacagctc acctcgcaat gttgggtggc gacggcagct
     3841 cgacgcacga cccgagccga cgcccggtca aggatgctat tatatggtgt gtcgcggggt
     3901 taagggccag atctcgccgc cggccggcaa gttttctggg cgccccctga cctgcggcac
     3961 gcaaggccgc cccttcgctg gggcgctcgt cagcaccgcg taggcttgag cagggtgcgg
     4021 catcgcccag aggagtacac tcggcgtaca ctccctgagg agtctcacga tgtgccaaga
     4081 ccgggatcgc tccgcaccag ccccgcctaa gggtttgacg ccacaagccg agcgtgcgct
     4141 cagggaagcc gaggaacgac gcctgcgggc gcaacagggc gccgcgccgc agcccaagga
     4201 gatcggcggg cggcaaggcc cggaccccac gcgctatggg gattgggaaa aaaacggcat
     4261 tgcgtccgat ttttaaatcg ggggaacgct gccaagcggt taaggtcggc aggttacctt
     4321 gccgcgaaga cgtggttcgc ctcgacacgg cgcgccgcgt gtggctcgcg cggcgcagga
     4381 ctggcgccgc acgccaaggc cggcttggcc ggtccatggc cgacctctat agaaggttgc
     4441 catgccgatc tcccccgagc ccaagccgcg ccatcgcctg atcgttcggc tcctcctcct
     4501 ggttttgctc gggctcggtg cgggcttcct gtttcggcag gggatgatcc cggccgtcgt
     4561 caaccctctg ccggcgctcg atctcagcca ggccgatccc tggttcgtcg attggcggct
     4621 tgccgccctg cggcacgacc cgggtctctg ccggcgcatg ctggcccaac ccgaaatccg
     4681 cgccgagccg atcgccgaca acccgcccaa agacggctgc ggctggacca acagcgtgcg
     4741 catgacggcg gccgggggcg cgcacgcggc tttcgacaag gcgacctgcg aggcggccac
     4801 ggcgctggcg ctgtggctaa cgcacggcgt gcagcccctg gctcaggaat tgctgggcca
     4861 gcgcgtgacc tcgatccaaa cctttgggtc ctacgcctgc cgcaacatcg tcggcaatcc
     4921 cttctggaag gacatccgca gcgagcacgc gaccgccaac gccctcgaca tctcgggatt
     4981 caccttggcc ggcgggcgcc agatcagcgt cgcccagcat tggaagggaa ccgggccgga
     5041 ggcgcgcttc ttgcgtgccg tgcacgcgcg tgcttgtcac tattttcacg tcgtgctggg
     5101 ccccgactat aatgccgcgc accacaatca ctttcacctc gaccgcggtc ttttctggcg
     5161 ctgcacttag tctctagcgc gcgccgaacc gcaaggggcc cggcagcagg cggcggccag
     5221 cgcctggcgc gctgcgccgc caatcctcgg cgctgtcgac atcgccgagc gtaccgagga
     5281 agccgatgcg gtggtccgac aaattggcaa gcgtatcgct gagcgcgtgc ggcgaggacc
     5341 aacgcaccgc acgaaacggc cgcggcaggc gcgggcgccg gcgcaagccg accaaccaat
     5401 agccaccatc gagcgccggg ccgaacacgg cgtcgcggtt gcccagcaga cgaaacgcgc
     5461 gcgcgatatg ctgcgccgtc aggttgggaa tgtcggtgcc cacgatgacg agcggccccg
     5521 gggccgtcgc ctggcagagg cgctgcatgc gctggccgag atcgccgggg ccctgcgcac
     5581 agattttgct cttggccggc cagatcgggc tatcggcaaa gcgatcggga ctgacggcca
     5641 ggatcgtctg ccagcgccta tcgcgtccca cccggctcat cagggctgag atcgcggcgc
     5701 gcgcgaagcg gatcgcctcc gtgctgccga tctcggcggc gagccggcgc ttggccgcgc
     5761 cggccagcgg cgctttcgcc atcacgacga gccggcagcg aaaaggcgcc gcccgcggcg
     5821 gcgcccccgg cagcgaggcc cgacggcgac gctgagccat gggccgtggc cgccgcctcg
     5881 ttccgcgact aggccttgac cggtacgaag gtatagccct tgccatcctt attgatgctg
     5941 cccgcacccg ggaagccgaa gtgatagccg gtcacggtga ccttgtcggc aatcacgcga
     6001 tcaaacatct tgcgccgatt ggtctccgcc agcaccgggt ccgcgtcaaa caccaagtgc
     6061 cagcctggat tcttcagatt gagggcgggg atgttggtga cgtcgccgag cacgatcagc
     6121 tgcttcttgc cggaggccag ctggtagctg gtgtgaccgg gcgtgtggcc gttgctggcg
     6181 atggggcgca cgcccggtgc gatctccttg tcgccttcga actgcttgat gttcttccag
     6241 ccgggaaaca ccgcttgaat gcgtttcgcc gcgccggccg tggtcgaggg atccgtccag
     6301 aatttgtatt cggcggccgg aaccaggatt tcggcgttgg gaaagatggg ggcgttggtg
     6361 tccttggcca tcaagccgct gatgtgatcg ccatggaagt gggtcacgac gatggttgag
     6421 actttgtcgg gatcgatgcc ggccgccttc atgttcttgg cggcaaccaa gcccgccttc
     6481 ggcgacacct ggccgccggt ccccgcatcg aacagcacga gcttgccctt gctcttgacg
     6541 gcggtgacgg tgaaggaaat gggcacgaac tcctcggtca agccgccggc tttgagagcg
     6601 gccttgacct cgtccagtga ggcatttttc acaaagttgg gatcgtgcgc cttttcccag
     6661 aggccgtcgt acatctgaat gacttcgatg tcgccaacct tgaatttgat aacgcccttg
     6721 tccatcagct tggggtcggg cttttgcgca aaggcgggcc cgacaaactc gagcggcccg
     6781 tccagaccga acacggcccc cgccgtcgcc gccgaaatca caaacccgcg gcgtgaaata
     6841 ctctgcatgg cactctccta gccgttttgc ccggtgaacg atatctgaca gacgtcacag
     6901 gcaggcccgg cattccctgt cacggacatt tgaaaggcgg ccgtgttccc tcagccatag
     6961 atgcggtgaa tggtgccgat gggcacgcgc agcaaataaa gggctaggca caagaggttg
     7021 cgtgccgagc ggcgcccata gccctcgcgc acgaaccgct ccgcactggt gatcgcgggg
     7081 gcgcgcaaca tgctcacccg ccgccgaccc agccggcgca cgaggtcgac gtcttccatc
     7141 aatagtaagg gccgatagcc accgagcgtc tgatacagcc gcttcggaat gagcaatccc
     7201 tgatcgccgt agggcaggcg cagaagcatg cagcgcagtg ccaccaggcg ctcgagcagc
     7261 cgcggccgca acccctgatc gtccagggcg aagcgaaagg ccgccgcgcc cagggggctg
     7321 tcttcactgt cgacccgccg caggaaggcc agcgcctccc gctcccagcc gtcccccagt
     7381 cgggtgtcgg cgtgcaggaa caggagccag ggaaagcgcg cttccttggc acccagggcc
     7441 aactggctgc cgcgtccgcg ggggccgacc aggacgcgcg ccccgctgct atcggcgatg
     7501 gccgcggtgg catcgcgcga accgccgtcg acgacaatga cctcgcgcac gaggccatcg
     7561 accgccgccg tcaccaggct ggcgagcgtc gccggcaggc tcgcctcgct gtcgagggtt
     7621 ggaatgacaa cggaaatcat cgcacggcaa tcatggactg cgccctgggc gttggctgag
     7681 ccctgatagc gccggccagc cgcggctcaa agtggatccg ttttgttctt tatttgttcc
     7741 gacctttgcc ctaatctttg cagccatggc caagaccgcg caggaaaccc gggacgagcg
     7801 gcggcgcgct ggcgcggccc gcgcgagccc ggccgtgggc gccccggccc cggcccattc
     7861 gcctgaagct gctgagggcg aagctgccct cgttgaggcc gagcgccggc gcgggcgtgg
     7921 cgcacgctcg aaccgcgtcg gtcgcttcga ggccgagttg cgggagagct tcgatgatgg
     7981 ctgggagggt ctcggcgatc ttgccccctt caagacccaa gtttttcgcg agaccgccaa
     8041 gagcatcatt gccaccaacg acagtcccga catcagcttc gatcagtcga tcaatcccta
     8101 ccggggctgt gaacacggct gcatctactg ctttgcgcgt ccaacgcatt gctatctcgg
     8161 ccactccgcc ggcctcgact tcgaaaccaa gctctacgcc aagatcaacg gcgcggatct
     8221 gctcgaacgc gaactggcgc attcgcgcta ccgagccaaa gtcatcgcgc tcggcgccgt
     8281 taccgatcct taccaaccga tcgagcgcga gcatcgcctg acccgcgcgc ttctcgaggt
     8341 gctcgatcgc accagccacc ccgtcggcat tgtcaccaag tcggcgttgg tcgtgcgcga
     8401 catcgatctc ctggctcgca tggccaagcg cgatctcgtg aaggtggcga tctcggtcac
     8461 cacactcgac cgacagatcg cgcgcaagat ggagccgcgc gcggccacgc cgggcaaacg
     8521 gctcgaggcc atcgccgcac tcgctgccgc aggggtgcct gtcgccgtga tggtcgcgcc
     8581 catcgtaccc gcgctcaatg acagcgagat cgagagcatt ttgagtgctg cgcagc
//
LOCUS       DASTMT010000355         2315 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_4365909, whole genome shotgun sequence.
ACCESSION   DASTMT010000355 DASTMT010000000
VERSION     DASTMT010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2315
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4365909"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            722..1690
                     /locus_tag="VFR00_08315"
     CDS             722..1690
                     /locus_tag="VFR00_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014131530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEU0159304.1"
                     /translation="MRSCIELACVIGALAFAQPAQAQDFPNRAIKFVVPFPAGGPADT
                     ISRILTEKMASLIGQSMVIENRAGAGGLTGIAAVAKAEPDGYTIAIAPSSGLAMNINL
                     RENMPFHPLKDLALITQIVSVPEILVVNEKVPVASLAELVSLAKSQPGKLVFASTGLG
                     GMPHLAMELLKLTAQIELVHVPYTGAAPAVNDLLGGHVQMMFADVPVLLGSIQSGKLR
                     ALAIGSKTRIASLPSVATTAELGMPQVEADNWYGLVAPIATPPAIVAKIHAAATQALQ
                     SDDVKDRLAAQGALAVGNSSAEFSGYVKSEIERWGGVIAATGIKIK"
     gene            complement(1700..>2315)
                     /locus_tag="VFR00_08320"
     CDS             complement(1700..>2315)
                     /locus_tag="VFR00_08320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018273484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU0159305.1"
                     /translation="KLLANLRAAGVAPTDIEAVLLTHMHPDHSAGLTDMETGKLNYPN
                     AELVVHENEPRHWFDDAKMASATERAKKLYFQAAREQTAPYQKRTRLFTAGEVFPGVT
                     AIPRPGHTPGHTTFLISSGNEQLLIWGDIVHVPEVQTARPEVCMEFDTDADAAAAQRR
                     KVFDMVATDRLLVTGMHMHFPGFAHLVRRGTGYQLIPAAWEQEL"
BASE COUNT          410 a          750 c          752 g          403 t
ORIGIN      
        1 gtcttaattg actttccccg caccgcgatt gaggcgacag ctgcagcagg ctcagtaccg
       61 ctccccaata ctttgcttat tcaactcaag gctgcgctcc aaagcgcagc cttgtttgct
      121 ggagacaccc cgcgcgccgc gggcgacatt caaggcgagc gcaagcgacc ttttccgcgc
      181 tcaacaacgc tcggtgccgg tgcgccgtct cctgcaagca caccctctct tgcaagcgcc
      241 atctctggtg agcgccgctc tgcaaaccgc ggggcgcgcg cgcgccgccg cccgatcctt
      301 tggcgcgacg acgggttgcc atggccgtgg gcgcgaagct gcgcggcaag cccgactgta
      361 attgcgcttt gaaactgtgc cgggcttgcg aggtgtgcag gcttgcagcg tgtggcggag
      421 gaagggagcc cgcgtgccgg gccagaaagc aggctcagcc ggcacagcgc gcggcgggcc
      481 gcctggcctc ggaatgttgg cgccaagaaa ttagggagga gggaagcaca agcctccctc
      541 gccccaggtc cagatctgcg gaccgtgcag caaccccgcg gtgccgcgct cccatcgaga
      601 gcggggagcc tccatcctaa gagcaaacct caagcggcgc cagcgccatc tcgcgaggcg
      661 gcgtccgcgc cgatatgcca tactcgtgga ggaacaatca ggcgcgagga gagggacgct
      721 aatgcgctca tgcatcgaac tagcttgcgt tatcggagcg ttggcctttg ctcagccggc
      781 gcaagctcag gattttccca atcgcgccat caagttcgtc gtgccgttcc cggccggggg
      841 ccccgccgac accatcagcc gcatcctgac ggagaagatg gcgagcctca tcggccaaag
      901 catggtgatc gaaaaccgag ccggcgccgg cggccttacc ggcattgctg cggtggccaa
      961 ggccgagccc gacggctaca ccatcgccat cgcgccttcg agcggactgg caatgaacat
     1021 caacctgcgc gagaacatgc cgtttcatcc gctcaaggat ctggccctca tcacccagat
     1081 tgtctcggtg ccggaaatcc ttgtcgtcaa cgaaaaggtc ccggtcgcct cgctggccga
     1141 gctcgtcagt ttggcaaaat cgcagccggg caagctggta ttcgcctcga cgggcctcgg
     1201 cggcatgccg catctcgcca tggagctgct caagctgacc gcacaaatcg agctggtgca
     1261 cgttccctat acgggtgccg cgcccgcggt caacgacttg ctcggtggtc acgtgcagat
     1321 gatgtttgcc gatgtgcccg tgctgctcgg tagcatccag tcgggcaagc tgcgggcgct
     1381 ggcgatcggc agcaagacgc gcattgcgag cctgccgtcg gtggccacca cggccgagct
     1441 cggcatgccg caggtagaag ccgacaattg gtatggcttg gttgcgccga ttgctacgcc
     1501 gccggcgatc gtcgccaaga tccacgccgc agccacgcag gcgctgcaat ccgacgacgt
     1561 caaggacagg ctcgctgcgc agggcgccct tgcggttggc aattcgtcgg ccgagttctc
     1621 cggctacgtc aagagcgaga tcgagcggtg gggcggcgtc atcgccgcca ccggcatcaa
     1681 gatcaaatag cgctcgcgcc tatagctcct gctcccaggc ggcggggatc aattgatagc
     1741 ccgtgccccg ccgaaccaag tgcgcgaacc ccgggaaatg catgtgcata ccggtgacga
     1801 gaaggcggtc ggtggcgacc atatcgaaca ccttgcggcg ctgcgcggcg gcggcatcgg
     1861 cgtcggtatc gaattccatg cacacttcgg gccgcgcggt ctgcacctcg ggcacgtgca
     1921 cgatgtcgcc ccagatcagt agctgctcat tgccggagct gatcagaaag gtggtatgac
     1981 ccggcgtgtg gccggggcgc gggatcgcgg tgacgccggg aaacacctcg cccgcggtga
     2041 ataggcgcgt gcgcttctgg tagggcgcgg tctgctcgcg tgcggcctgg aaataaagtt
     2101 tcttggcccg ctcggtagcg ctcgccatct tggcgtcgtc gaaccagtga cgcggctcgt
     2161 tctcgtgcac gacgagctcg gcattggggt agttcagttt gccggtctcc atatcggtga
     2221 ggccggccga atgatcggga tgcatgtgcg tcagcaatac cgcttcgatg tcggtcggcg
     2281 cgacgcccgc cgcgcgtaag ttggccagca acttg
//
LOCUS       DASTMT010000356         6970 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_6334416, whole genome shotgun sequence.
ACCESSION   DASTMT010000356 DASTMT010000000
VERSION     DASTMT010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 6970)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6970)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6970
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6334416"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..664
                     /locus_tag="VFR00_08325"
     CDS             <1..664
                     /locus_tag="VFR00_08325"
                     /inference="COORDINATES: protein motif:HMM:NF017743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0159306.1"
                     /translation="SERRLAGSAATRLAQIAAGERGLARLALNGETLSQRSLPQLSFA
                     GVEVSPPPGAFVQAVAAAEALLGEEVTNAVRGARRPADLFCGLGTLTFALARRAAVAA
                     FDHDAEAIAALLTAARHAKGLKPITASVRDLLREPLAPGELKDYDAVVFDPPRAGAKA
                     QALALARSRVPVVVAVSCAPGTLARDLKILVDGGYRLQGVVPIDQFIFSAHVEAVAHL
                     RR"
     gene            complement(714..1166)
                     /locus_tag="VFR00_08330"
     CDS             complement(714..1166)
                     /locus_tag="VFR00_08330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0159307.1"
                     /translation="MRAGLIGIIALLLACAGPSRGTGEPGCSKADFEAVVDEAAAALR
                     ALNHQNTPQFQAKLRQLKDKRGWSHEQFLVEAAPFVRDDAIAGFDQKSEDFLARITQA
                     GQAQATAASLNCGLLVDLRGSLAHLVETQKAKWTYMFEKLNAELAKAR"
     gene            complement(1252..3498)
                     /gene="parC"
                     /locus_tag="VFR00_08335"
     CDS             complement(1252..3498)
                     /gene="parC"
                     /locus_tag="VFR00_08335"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013419052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HEU0159308.1"
                     /translation="MSKTTILAPPPPIEPIKLKDALEERYLAYALSTIMHRALPDVRD
                     GLKPVHRRILYAMRELRLDPEGGYKKCAKIVGEVMGNYHPHGDQAIYDALVRLAQDFA
                     VRYPLVDGQGNFGNVDGDNAAAMRYTESRLTSVAALLLEGIDENTVDFRPTYDGSNQE
                     PVVLPANFPNLLANGTSGIAVGMATSVPPHNVEELLNAALHLIKHPNAHVEKLVEFVP
                     GPDFPTGGVIVEPREAILEAYKTGRGGFRLRARWEKEEGGRGTYQIVVTEVPYQVQKA
                     KLVERIAELINDKKLPLLGDVRDESAETVRLVLEPKSRSVDPLLLMESLFRLTELEVR
                     SSLNMNVLTGQVPGVLSLRDVLRRWLEHRQVVLVRRSKFRLEKIEHRLEVLDGYLIAY
                     LNIDEVIRIVRFEDDPKAKLIKRFKLSEVQADAVLNLRLKSLSKLEEVEIRAEHDKLS
                     KERRGLKSLLKHEDEQWAEIAEQIKATRETYAKKTELGRRRSSFADAPAIAVDLDAAM
                     IEKEPITVILSEKGWIRAMKGHMEDTSKLEFKQGDALKRAVKASTTDRLLLLASNGKV
                     FTLAADQLPGGRGHGEPVRLMVDLEENHDFVEIWVHEPGRKLLVGATSGHGFIVPEDE
                     MVAMTRKGKQVMNVDPPAEAITAVPAEGDMVAAIGENRKMLIFPLDEVNEMSRGKGVI
                     LQRYKDGGLCDLRVFKKRDGLSWLDAAGRTFTLSTAELRDWLGQRAQSGRLAPKGFPR
                     SNKFGWAF"
     gene            3769..4446
                     /locus_tag="VFR00_08340"
     CDS             3769..4446
                     /locus_tag="VFR00_08340"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HEU0159309.1"
                     /translation="MPVLPDLPQTEIAIIEGTNAFRKEHNLGALKPNAALTAAARAFA
                     AYLGKTGKFAHEADGREPHQRAEAQGYRHCLIAENLAFNLDSRGFASAQLARDVVEGW
                     KKSPPHRANLLEKSATEIGVGVARATDRQPKFISVEVLGRPESLQVNFSIENRTGAAV
                     HYRFGEESDWVPARASVSHTSCAVGPLMIEKAAPAAGLEPRNGETFVLSPGPAGSIKV
                     ERGRTSP"
     gene            complement(4482..5069)
                     /locus_tag="VFR00_08345"
     CDS             complement(4482..5069)
                     /locus_tag="VFR00_08345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0159310.1"
                     /translation="MSLLKSWRDYPALVLASLGLVSGILSAVVGFELRIDVLDWPRSV
                     FLFPSVEMMPIGLFFAAVMAFAIAAWESPAWAPIITFVGTLYAWSAAIQTALFVHKIG
                     GGDASHVGPLLAGICAGFVGAALTHLSVALFARELFDRTHAARTCLVGAIAGILFYFG
                     SRDLIDARILFLVWQPAVAYAIGLGLATKPATQAV"
     gene            complement(5118..5843)
                     /gene="recO"
                     /locus_tag="VFR00_08350"
     CDS             complement(5118..5843)
                     /gene="recO"
                     /locus_tag="VFR00_08350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019170587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEU0159311.1"
                     /translation="MEWSDEGMILSVRPHGETGAVLELFTRHNGRHLGLVHGGRSRKL
                     RPILQIGNHVEASWKARIADNLGHFAVELRKGYAALVLDDAAALAALSSIAALARLLP
                     ERDPHPNLFEVTLFVLGYLDEPAVWPALLVRWELALLEELGFGLDLSACAATGTRQDL
                     IYVSPKSGRAVSAAAGAPYKERLLALPQFLIGSPEAGVTATDVVLGLALTEHFLLARV
                     LAPRDLTMPEARNRLLSYLRPNV"
     gene            complement(6005..6919)
                     /gene="era"
                     /locus_tag="VFR00_08355"
     CDS             complement(6005..6919)
                     /gene="era"
                     /locus_tag="VFR00_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011503058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEU0159312.1"
                     /translation="MSEPAPDAETRCGFIAIIGAPNAGKSTLVNALVGSKVSIVSHKV
                     QTTRVPLRGIAIAGASQLVFIDTPGLFQPRRRLERAMVEAAWTGAEDADLVVLMLDAA
                     LGLDAGAQRILDALKARKVAPILVLNKIDRVPKPRLLSLAAEANARLPFAATFMLSAL
                     DGNGVGDLKTYLARQVPPGPWHFPEDEVSDAPLRLLAAEITREKIYARLHDELPYRTA
                     VETTAWQEKKNGVRIEQTIFVERDSQKRIVLGKGGQMIKQLSSESRQELAQILEQPVH
                     LFLFVKVRQDWEDDPERYRQMGLSFPKN"
BASE COUNT         1122 a         2268 c         2375 g         1205 t
ORIGIN      
        1 cagcgagcgg cgcctcgcgg gcagcgcggc cacgcgcttg gcgcagatcg ccgccggcga
       61 gcgcggcctc gcccgtcttg cgctgaacgg ggaaacgctg agccaacgca gcttgccgca
      121 gctctccttt gccggcgtcg aggtgtcccc gccgcccggc gcgttcgtgc aggccgtggc
      181 ggcggcggaa gctctgcttg gcgaggaggt aaccaacgcc gtgcggggcg cccgccgccc
      241 cgccgatctc ttctgtggcc ttggtacgct cacctttgca ctcgcgcgca gagcagccgt
      301 tgcggccttc gaccacgacg cggaggccat cgcggcgttg ctgaccgccg cccgccacgc
      361 caagggcttg aagccgatca cggccagcgt gcgcgatctc ctgcgcgagc cgctggcgcc
      421 gggtgagctc aaggactatg acgccgtcgt gttcgatccg ccgcgcgcgg gggccaaggc
      481 gcaagcgctg gcgctggccc gttcgcgcgt tcccgtggtc gtggcggtct cttgcgcgcc
      541 gggcacgctc gcgcgtgatt tgaagatctt ggtcgacggc ggctatcgct tgcagggcgt
      601 ggtgccgatc gatcagttca tcttttcggc ccacgtcgag gccgtcgcgc atctgcggcg
      661 ctgagccgcc gcatgcgcag gcttgcgcgc cgcgccgcgc gagagcccgg gagttagcgc
      721 gccttggcga gctcggcgtt gagtttctcg aacatgtagg tccacttggc tttttgtgtc
      781 tcgaccaagt gggccaggct gcccctgagg tcgaccagca ggccgcaatt gagcgaggcg
      841 gcggtggcct gggcctggcc tgcctgggtg atgcgggcga gaaaatcctc ggacttctgg
      901 tcgaagccgg caatggcgtc gtcgcgcaca aagggggccg cctcgaccag gaattgctcg
      961 tggctccagc cccgcttgtc cttcagctga cgcagcttgg cctgaaattg gggggtgttt
     1021 tgatggttga gcgcgcgcag cgcggccgcc gcctcgtcga ccacggcctc gaagtcggcc
     1081 ttgctacagc cgggctcccc cgtgccgcga ctggggccgg cgcaggccaa caagagcgcg
     1141 atgatcccga tcaggcccgc ccgcatgtgc ttgtctctcc gccggctcca aacaccctgt
     1201 cagcatccca gcggccactc accgaagctt ggcgtggcgt gggcgccctt ttcagaaggc
     1261 ccagccgaac ttgttggagc ggggaaatcc cttgggcgcc aaccggccgg actgggcccg
     1321 ctggcccagc cagtcgcgca gctccgccgt cgagagcgtg aacgtccgcc ctgccgcgtc
     1381 gagccagctc aagccatccc tctttttgaa gacgcgcagg tcgcagaggc caccatcctt
     1441 atagcgctgc agaatgacac ccttgccgcg cgacatctcg ttgacctcgt cgagcgggaa
     1501 gatcaacatt ttccggttct cgccgatggc cgccaccatg tcgccttcgg ccggcaccgc
     1561 tgtgatcgcc tcggcggggg gatcgacgtt catcacctgc ttgcccttgc gcgtcatggc
     1621 gaccatctcg tcctcgggca cgatgaagcc gtgcccggag gtggcgccca ccaatagctt
     1681 gcggcccggc tcgtgcaccc agatttcgac gaagtcgtgg ttctcttcca gatccaccat
     1741 cagccgcacg ggttcgccgt gcccgcgccc gccgggcagc tggtcggcgg ccagcgtgaa
     1801 caccttgccg ttgctggcaa gcaaaagcag ccggtcggtg gtcgaggcct tgacggcgcg
     1861 tttcagcgca tcgccctgtt tgaactcgag cttggaggtg tcctccatgt ggcccttcat
     1921 ggccctgatc cagccctttt ccgagaggat caccgtgatt ggctccttct cgatcatggc
     1981 tgcgtcgagg tcgaccgcga tggcgggggc atcggcaaag gaggagcggc gccggccgag
     2041 ctcggttttc ttggcgtagg tctcccgcgt cgccttgatt tgctcggcga tctcggccca
     2101 ctgctcgtcc tcgtgcttca gcagcgattt gaggccgcgc cgctctttgc tgagcttgtc
     2161 gtgttcggcc cggatctcga cctcttccag cttggacagc gatttcaagc gcagattgag
     2221 gaccgcatcg gcctgcacct cgctcagctt gaagcgcttg atcagcttgg ccttggggtc
     2281 atcctcgaag cgcacaatgc ggatcacctc gtcgatgttg aggtaggcga tcaggtaacc
     2341 gtcgagcact tcgaggcgat gctcgatttt ctccaggcgg aatttggagc gccgcaccag
     2401 cacgacctgc cggtgctcca accagcgccg caaaacatcg cgcagcgaga gcacgccggg
     2461 cacctgtccc gtcagcacgt tcatgttgag cgaggagcgc acctcgagct cggtgaggcg
     2521 aaacaagctc tccatcaaca gcagcgggtc gacgctgcgg cttttcggct ccagcaccag
     2581 gcgcacggtt tccgccgact catcgcgcac atccccgagc aagggcagct tcttgtcgtt
     2641 gatcagttcg gcgatgcgct cgaccagctt ggccttctgc acctggtagg gcacctcggt
     2701 gacgacgatt tgataggtgc cgcggccgcc ctcctccttt tcccaacgcg cgcgcaggcg
     2761 aaagccgccg cggccggttt tataggcttc caggatggct tcgcgcggct ccacgatcac
     2821 gccgccggtg ggaaagtccg ggccgggcac gaactcgacc agcttttcga cgtgcgcgtt
     2881 cggatgtttg atgaggtgga gcgccgcgtt caacagctct tcgacattat gtggtggcac
     2941 gctggtcgcc atgccgacgg caatccccga cgtgccgttg gccagcagat tcgggaaatt
     3001 ggcgggcaag accaccggtt cttggttgct gccgtcgtag gtgggtctaa aatcgacggt
     3061 gttctcgtcg atgccttcca gcagcagcgc ggcgacgctg gtgagccggc tttccgtata
     3121 acgcatggcc gcggcgttat cgccgtcgac gttgccgaaa ttgccctggc cgtccaccag
     3181 cgggtagcgc acagcaaaat cctgcgccag ccgcaccagc gcatcgtaaa tcgcctgatc
     3241 gccgtgcggg tgatagttgc ccatcacctc gcccacgatc ttggcgcatt tcttgtagcc
     3301 cccttcgggg tcgagccgca gctcgcgcat ggcataaagg atgcgccggt gcaccggctt
     3361 caagccgtcg cgcacgtcgg gcaaggcgcg atgcatgatg gtggagagcg cgtaggcgag
     3421 gtagcgctcc tcgagcgcgt ccttgagctt gatgggctcg atgggtggag ggggggcgag
     3481 aatcgtggtt ttgctcatgc ggcctgggtt agcccaggac gcccggcggc tcaagggaat
     3541 cgccgggcgc ttaggggggt gctttcgccg gggtgttggg cgtgttgccg ccgccgagcg
     3601 cgccgacggg tggatcccag agccggggcg gctggcggga acggccatcc gccggcggca
     3661 ttgcgccgtt ttggcggctt tctgcggcgg caggctttac gcggcggcag gctttgagtc
     3721 tttcgcgagc tttgagtgtt ttgcccggtg tttgctagag gttttgggat gcccgtgctg
     3781 cccgacctgc cccagacgga gatcgccatt atcgagggga cgaatgcctt tcgcaaagag
     3841 cacaacctgg gcgcgctcaa gccgaatgcg gcgttgacgg cggcggcgcg ggcctttgcc
     3901 gcctacctcg gcaaaaccgg gaaattcgcg cacgaggccg atggccgcga gccgcaccag
     3961 cgcgccgagg cccagggcta tcgccattgc ctgatcgcgg aaaacctggc cttcaatctc
     4021 gacagccgcg gctttgcgag cgcgcaactg gcacgcgacg tcgtcgaggg ttggaaaaag
     4081 tcgcccccgc atcgcgccaa cttgctggaa aaaagcgcca ccgagatcgg cgtcggcgtg
     4141 gcccgcgcca cggaccgcca gccgaaattc atttccgtcg aggtgttagg tcggccggag
     4201 tcgctgcagg tcaatttttc catcgagaac cgcacgggcg ccgccgtcca ctaccggttc
     4261 ggcgaggaga gcgattgggt gccggcgcgc gccagcgtta gccacacgag ctgcgcggtc
     4321 ggtccactaa tgatcgagaa ggcggcgccg gcggcggggc tcgaaccccg caacggcgag
     4381 accttcgtcc tgagcccagg accggcgggg tccatcaagg tcgagcgcgg gcgcacgtcg
     4441 ccgtagccgc gcggcgacag ggacgcgcgc ggggacgccg cctagacggc ctgggttgcg
     4501 ggtttggttg ccagtccgag gccgatggcg taggccaccg ccggctgcca gaccaaaaac
     4561 agaatgcgcg cgtcgatcag gtcgcggctg ccgaaataga acaaaatgcc agcgatggcg
     4621 ccgaccaggc aggtgcgcgc ggcatgggtg cgatcgaaga gctcgcgggc aaaaagcgcc
     4681 accgacaggt gcgtgagcgc cgcgccgaca aagccggcgc agatgccggc caacagcggc
     4741 ccgacatggc tggcatcgcc gccgccgatt ttatgcacga acagggcggt ctggatggca
     4801 gcgctccagg cataaagcgt gccgacgaag gtgatgatgg gggcccaggc ggggctctcc
     4861 caggcggcga tggcaaaggc catgacggcg gcgaaaaaca agccgatcgg catcatttct
     4921 acggagggaa atagaaacac gctgcgcggc cagtccaaaa cgtcgatgcg cagctcgaag
     4981 ccgacgacgg cggacaggat gccggagacg aggccgaggc tcgccaacac gagcgccggg
     5041 tagtcacgcc aggatttcag caacgacacg ggtttcatgg tccttgcgcg ggtcggaagc
     5101 gactgcgacg tcgatagcta cacattcggc cgaaggtaag acaacaagcg gtttcttgcc
     5161 tccggcatgg tgaggtcgcg cggggccagc acgcgcgcca gtaggaaatg ctcggtcagc
     5221 gccaggccga gcacgacgtc ggtcgcggtg acgcccgcct ctgggctgcc gatcagaaat
     5281 tggggcaagg ccagcagccg ctccttgtag ggcgcgccgg ccgccgccga gaccgcccgc
     5341 cccgacttgg gcgacacata gatgaggtcc tgcctggtcc cggttgcggc gcaggctgac
     5401 agatcgaggc cgaagcccag ctcctcgagc agtgccagct cccagcgcac cagcagcgcg
     5461 ggccacaccg ccggctcgtc caagtagccg agcacaaaca gcgtgacctc gaacagattg
     5521 gggtggggat cgcgctccgg cagcagccgg gcgagcgcgg caatcgagct caaggcggcc
     5581 agcgcggcgg catcgtcgag cacgagcgcg gcatagcctt tgcgcaactc cacggcgaag
     5641 tggccgaggt tgtcggcaat gcgcgccttc cagctggcct cgacgtgatt gccgatctgc
     5701 aggatgggcc gcagcttgcg cgagcggccg ccgtgcacga gacccaggtg gcgcccgtta
     5761 tggcgcgtaa agagctcgag tacggcgccg gtctccccgt gcggccgcac gctgaggatc
     5821 atgccttcgt ccgaccactc catggcgagc ttagcccccg ggccgagcgc tgccgcggca
     5881 gccctgcggc gaggcgccgg gcgcctggcg cggcagttgg gcggagaggc gggcgacctg
     5941 gtgcatcctg gtggatcttt gtgagcgcgt catgctgccg gcgcgcggct tttccacggc
     6001 gactttagtt tttgggaaaa ctcaaaccca tctgccggta gcgttccgga tcgtcctccc
     6061 agtcctggcg caccttgacg aacaggaaga gatgcacggg ttgttcgaga atttgcgcca
     6121 gttcctggcg ggactcgctg gagagctgtt tgatcatctg gccgcccttg ccgagcacga
     6181 tgcgtttttg gctgtcccgc tccacgaaaa tggtctgctc gatgcgcacg ccgttttttt
     6241 tctcctgcca ggcggtggtt tccaccgccg tgcggtaggg gagttcgtcg tgcaggcggg
     6301 cgtagatctt ctcgcgcgtg atctcggccg ccaacaggcg cagcggcgca tccgagacct
     6361 catcctcggg aaaatgccag gggccgggcg gcacctggcg ggcgagatag gttttgaggt
     6421 cccccacccc gttaccatcg agcgccgaga gcataaaggt ggcggcgaag ggcaggcgcg
     6481 cgttggcctc ggccgccagg ctgagcagcc gcggcttggg cacgcgatcg attttattga
     6541 gcaccagaat aggggccact ttcctggcct tgagcgcgtc caaaatgcgt tgcgctccag
     6601 cgtcgaggcc cagagcggcg tccagcatca ggacaacgag atcggcatcc tccgccccgg
     6661 tccaggccgc ctccaccatg gcgcgctcca ggcgccggcg cggctgaaaa aggcccggcg
     6721 tgtcgatgaa gacgagctgg cttgccccgg caatggcaat gccgcgcaag ggcacgcgcg
     6781 tggtctgcac cttatggctg acgatcgaga ccttgctgcc caccagggcg ttgaccagcg
     6841 tcgacttgcc ggcgttggga gcgccgatga tggcaataaa gccgcagcgg gtttcggcgt
     6901 cgggcgcggg ctcgctcact tggcgtgcct ccagacgccc tcgcgcgaga gcagcgcggc
     6961 ggcggcggcc
//
LOCUS       DASTMT010000357         3187 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_853660, whole genome shotgun sequence.
ACCESSION   DASTMT010000357 DASTMT010000000
VERSION     DASTMT010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3187
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_853660"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..930)
                     /gene="argF"
                     /locus_tag="VFR00_08360"
     CDS             complement(<1..930)
                     /gene="argF"
                     /locus_tag="VFR00_08360"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003493698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEU0159313.1"
                     /translation="MSMAPRHFLDIDRVDAATLRRIVDMGHAMKRAGKRVPARLKPAG
                     IADAVLMLIFEKPSTRTRVSFDVAMRQLGGQTIALNHTDLQVGRGEPISDTAKVISRY
                     VDAIMIRANKHETLMEFAEHASVPVINGLTDRTHPCQIIADIMTFEEALGPIKGRKVA
                     WTGDGNNVAASWIQAAARFDFKLALACPPQLNPERSVLEWTRQAGGDVHLTEEPQEAV
                     AGADCVVTDTWVSMGQQHEASRRKQLLGAYAVDESLLRRAHKDAIFMHCLPAYRGLEV
                     AASVIDGAQSVVWEEAENRLHAQKAILAWCLGVD"
     gene            complement(958..2124)
                     /locus_tag="VFR00_08365"
     CDS             complement(958..2124)
                     /locus_tag="VFR00_08365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019172944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEU0159314.1"
                     /translation="MPTYARQNVVFERGEGAWLIATSGERYLDFASGVAVNALGHTHP
                     KLIAALTEQAHKLWHSSNLYRVAGQERLAERLCAATFADKVFFCNSGAEACEGAIKLA
                     RRYHFANGAPERWRIITFKGAFHGRTLATIAAAGNEKHLEGFGQPADGFDLLALGDPD
                     AVANAIGPHTAAIMIEPVQGEGGARVVEPPFLKALRQLADKHGLLLILDEVQSGMGRT
                     GKLFAHEWAGITPDVMAIAKGIGGGFPVGAFLASNEAAKGMVPGTHGSTFGGNPLAMA
                     VGNAVLDAILEPGFLEHVQAISLRFKQELARIRDEFPNLVEEVRGSGLLTGIKVKPPY
                     GDVVNACTAEKLLTVGAGDNVVRLLPPLNVTEAEITECTRRLAAALRRLAKPAS"
     gene            complement(2498..3094)
                     /locus_tag="VFR00_08370"
     CDS             complement(2498..3094)
                     /locus_tag="VFR00_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GcrA family cell cycle regulator"
                     /protein_id="HEU0159315.1"
                     /translation="MAWNDERVELLKKLWADGLSASQIAGRLGGVTRNAVIGKVHRLG
                     LSGRATTSRMKSHRPRARVQSAKRLMKPRFANVGNPALRNLYLGDTEPYTPPAEELEI
                     PINERKYIQTLTEISCRWPIGDPQHTDFHFCGKSKVTGLPYCEFHARRAFQPPQPRRR
                     DREVPAPMLVVGPGSVEPVATPREPAGQPSGSKLKVAV"
BASE COUNT          539 a         1033 c         1050 g          565 t
ORIGIN      
        1 atcgacccca aggcaccagg ccaggattgc cttttgcgcg tgcagacggt tctcggcctc
       61 ttcccacacc accgattgcg ctccgtcgat aacgctggcg gccacttcca gcccgcgata
      121 ggcaggcagg caatgcatga aaatcgcgtc cttgtgggcc cggcgcagca ggctctcgtc
      181 gaccgcatag gccccgagca gctgcttgcg tcgcgatgcc tcgtgttgct ggcccatcga
      241 cacccaggtg tcggtgacga cgcagtcggc gcccgcgacc gcctcttgcg gctcctccgt
      301 cagatgaacg tcaccaccgg cttgacgcgt ccactccagc accgaccttt ctgggttgag
      361 ctgcggtggg cacgccagcg cgagcttgaa atcgaagcgg gcggccgcct ggatccagga
      421 ggccgcgacg ttgttgccat cgccggtcca ggccaccttg cgacccttga tcggccccag
      481 cgcctcttcg aacgtcatga tatcagcaat gatctggcag ggatgcgtgc gatcggtgag
      541 cccattgatg acgggcaccg acgcgtgttc ggcaaattcc atcaaggtct cgtgcttgtt
      601 ggcccggatc atgatcgcat cgacgtagcg cgaaatgact ttggccgtat cggatatggg
      661 ctcgccccgc ccgacctgca agtccgtgtg gttcagcgcg atcgtttgtc cgccaagctg
      721 acgcatggca acgtcgaagg acacgcgcgt gcgcgtggaa ggcttctcga aaatcaacat
      781 cagcacggcg tcggcgatgc cggcgggctt aagtctcgcc ggcacccgct tccccgcccg
      841 cttcatggcg tgacccatgt cgacgatacg ccgcagcgtg gcggcatcga cgcgatcgat
      901 atccagaaag tgccgaggtg ccatcgacat ggcgacgtct ctcaaggccg cgcgcgctca
      961 cgacgccggc ttggcgaggc ggcgcaaggc ggccgcaagc ctgcgcgtgc attccgtgat
     1021 ttcggcctcc gtcacgttga gcgggggcag caggcgcacg acattgtcac cggcgcccac
     1081 cgtcaagagc ttttcggcgg tgcaggcgtt gacgacgtcc ccgtacggcg gcttgacctt
     1141 gatcccggtc agcagcccgc tgccgcgcac ctcctcgacc aagttcggaa actcatccct
     1201 gatacgggcc agctcctgtt taaagcgcag ggaaatggct tgcacatgct cgagaaaacc
     1261 cggctcgagg atggcatcga gtacggcgtt gcccaccgcc atggcaaggg ggttgccgcc
     1321 gaaggtcgag ccatgggtgc ccggtaccat acccttggcc gcctcgttgc tcgccagaaa
     1381 agccccgacc ggaaaaccgc cgccgatgcc tttggcgatg gccatcacgt cgggcgtgat
     1441 gccggcccat tcgtgggcaa acagcttgcc cgtgcggccc atgccgcttt ggacttcatc
     1501 caagatcagc aacaggccgt gcttgtcggc gagctggcgc aacgctttta ggaacggcgg
     1561 ctccacgacg cgggcgccgc cttccccttg caccggctcg atcatgatgg cggccgtatg
     1621 cggaccgatg gcgttagcga cggcgtccgg gtcgccgagc gcgaggagat cgaaaccgtc
     1681 agccggctgg ccgaacccct ccaagtgctt ctcgttgccg gccgccgcaa tggtggcaag
     1741 cgtgcgtccg tgaaaggccc ctttgaaggt gatgatccgc cagcgctcgg gcgcaccatt
     1801 ggcgaagtga tagcggcggg cgagcttaat ggcaccctcg caggcttccg cgcccgagtt
     1861 gcagaagaag accttgtcgg cgaaggttgc cgcgcacagg cgctcggcaa gccgttcctg
     1921 gccggcgacg cgataaagat tggaagagtg ccacagcttg tgcgcctgtt ccgtgagcgc
     1981 ggcaatcaac ttcgggtgcg tatggcccaa ggcgttcaca gccacgccgc tggcaaagtc
     2041 gaggtagcgc tcgccgcttg tcgcaatcag ccaggcgccc tcaccccgct cgaacaccac
     2101 attttggcgg gcgtaggtcg gcatgacggc ggcggagggg gccgacgcgg cggatggttt
     2161 gttggcgggg tgcttggcgg cttgctgggc ggtgcttgac atggcagaga cctatctcgt
     2221 gacagaaaac gaaaaaagcc gcgcttaggc gcggcgctgt gtgacgatcc ggcaaggctt
     2281 cgtcatcgca gccgcgcaaa cgcgcgagcg ccaagacgtc ggttgcgggg gatcgcatag
     2341 gtcatggcgc tcttatgagg ctggcggctg gcggtgtcaa tggcgtgcca gcttgcgccg
     2401 ccccgcgccg caaaaaatga gggcccggag ccgcctcggc gctcctggcc ctctccccgc
     2461 tgcttgcggc caacccctcg tgggggcaaa ccgatgtcta gacggcaact ttcaacttgc
     2521 tgccggaggg ttgcccggca ggctcgcgtg gcgtcgcgac cggctcgacg ctgcctgggc
     2581 cgacgaccaa catcggcgcc ggcacctcgc ggtcgcgccg acgcggctgc ggcggctgga
     2641 aggcgcgccg ggcgtgaaat tcgcagtaag gcaagccggt taccttgctc ttgccgcaga
     2701 aatggaagtc cgtgtgctgg ggatcaccga tcggccagcg gcaggaaatc tccgtcagcg
     2761 tctgaatgta tttgcgctcg ttgatgggga tctcgagttc ctcggccggc ggcgtgtagg
     2821 gttcggtatc gcccaggtag aggttgcgca gcgcggggtt gccgacgttg gcaaagcgcg
     2881 gcttcatcag tcgtttggcc gattgcacgc gggcccgcgg ccgatgcgac ttcatgcgcg
     2941 atgtggtggc gcgacccgac aggcccaaac ggtgcacctt gccgatcaca gcgttgcggg
     3001 tgacgccgcc gagacggccg gcaatctggc tggcgctgag gccgtcggcc caaagcttct
     3061 tcaagagttc gacgcgttca tcgttccagg ccataccctg gtttctcccg tgtgcacaac
     3121 ggctgaaccc ggcgcgactt gcgcagattc ccgcctactc cggacgagcc tggaaagacg
     3181 atcgggc
//
LOCUS       DASTMT010000358         1725 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_2818832, whole genome shotgun sequence.
ACCESSION   DASTMT010000358 DASTMT010000000
VERSION     DASTMT010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 1725)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1725)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1725
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2818832"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..492)
                     /locus_tag="VFR00_08375"
     CDS             complement(<1..492)
                     /locus_tag="VFR00_08375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEU0159316.1"
                     /translation="MGSWLSEREQRLVAGAETASAATPIPTQIVSNGEFLPPPQSETQ
                     KRVERRILELADDHGKRLGLSRRQFMQTGCGMAAAFLAMNEIYGSSVFQVDAAEAREP
                     EVTRARAQSLSGQFVFDVQTHFVRDDFEHKELLDLAKFAAQHWNTALKGDTDSLVRFK
                     FQNY"
     gene            complement(774..>1725)
                     /locus_tag="VFR00_08380"
     CDS             complement(774..>1725)
                     /locus_tag="VFR00_08380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008962980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine methyltransferase"
                     /protein_id="HEU0159317.1"
                     /translation="KSVPRVRMDIWRVPEPWLEQAQDDATLLAIRDMERAGIDIITDG
                     EIRRESYSNRFATALEGIDTANPGQVKARSGHATPVPRVVGKIRRREAVEVRDIKFLR
                     ANTERVAKITLPGPFTMSQQAVNEFYRDDEELVLDYAAAVNAEALDLERAGADIIQLD
                     EPWLRNDPEAAKRIAVKAINRALQGLKVPTIVHLCFGYAALVPGETKPVGYSFLPQLA
                     DSVAGAISIEAAQPKLDLGVLKELAGKQVLLGVIDLNEPTAETAEVVADRIRAGLKFL
                     TPDKLVPAPDCGMKYLPRALAFAKLEALSAGAAMVRAELA"
BASE COUNT          283 a          562 c          562 g          318 t
ORIGIN      
        1 gtagttctgg aacttgaagc ggaccaagga atcggtgtcg cccttgagcg ccgtgttcca
       61 gtgctgcgcc gcgaatttgg ccaggtccag caactccttg tgctcgaaat catcgcgtac
      121 gaagtgggtc tggacatcga acacgaactg gccgctgagg ctctgggccc gcgcccgcgt
      181 cacctccggc tcgcgcgcct ccgccgcgtc gacctgaaac acgctggagc cgtaaatctc
      241 gttcatggcg agaaaggccg cggccatgcc gcaaccggtc tgcatgaact gccgacggct
      301 caagcccagg cgcttgccgt ggtcgtcggc cagctcgagg atgcgccgct cgacgcgctt
      361 ttgcgtttcg ctttgcggcg gcggcagaaa ctcgccgttg gagactattt gtgtcggtat
      421 cggcgtggcc gccgatgccg tttcggcgcc tgccaccaaa cgttgctctc gttcactcag
      481 ccaactgccc atcgtcggtc tccccaatat gaacttatcg attaggtaga gcccactggc
      541 ctttcgcgtt cgacaagatt gcattcgaca atatcaggac gcgcgtggcc gcaaatcagc
      601 aggcccattg cccctcctag gcgaggaata aggggagaat atgatgtgta acccgtttcg
      661 ggcatcacat cgcgtcatgc tgcgagcgtc cgccgccgca gcaacgcaac ggtggcaccg
      721 gcgccgctga gccgggcgca gcggcccgcc cgcttaatcg ccgctggccc cgactaggcg
      781 agctcggcgc gcaccatggc ggcgcccgca ctcaaggcct cgagcttggc gaaggccaag
      841 gcacgcggca agtatttcat gccgcagtcc ggcgctggca ccagtttgtc gggcgtcaag
      901 aatttgaggc cggcccggat gcggtccgcc acgacctccg cggtttccgc ggtcggctcg
      961 ttgagatcga tgacgccgag caggacctgc ttgccggcga gctccttgag cacgcccaga
     1021 tcaagctttg gctgcgccgc ctcgatcgag atggcgccgg cgacgctatc ggcgagctgt
     1081 ggcaggaacg agtagccgac gggtttggtc tcgcccggca ccaaggccgc atagccgaag
     1141 caaaggtgca cgatggtcgg caccttcaag ccctgcagcg cccggttgat ggctttgacg
     1201 gcgatgcgct tggccgcctc gggatcgttg cgcaaccatg gttcatcgag ctggatgatg
     1261 tcggccccgg cgcgctccaa gtccagcgcc tccgcgttca cggcggcggc gtagtccagc
     1321 accaactcct cgtcgtcccg gtagaactcg ttgaccgcct gctggctcat ggtgaagggg
     1381 cccggcaggg tgatcttggc gacgcgctcg gtattggcgc gcaggaactt gatatcgcgc
     1441 acctcgaccg cctcccggcg gcggattttg ccgaccacgc gcggcaccgg cgtggcgtga
     1501 ccggagcggg ctttgacctg gcccgggttg gccgtatcga tgccctcaag ggcggtggca
     1561 aagcgattgg agtagctttc gcgacggatc tcgccgtcgg taatgatgtc gatgccggcg
     1621 cgctccatgt cgcggatggc cagcagggtg gcatcgtcct gggcctgttc gagccagggc
     1681 tccggcacgc gccaaatgtc catgcgcacg cgcggcacgg atttc
//
LOCUS       DASTMT010000359         2215 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_3150314, whole genome shotgun sequence.
ACCESSION   DASTMT010000359 DASTMT010000000
VERSION     DASTMT010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2215
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3150314"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..985
                     /locus_tag="VFR00_08385"
     CDS             <1..985
                     /locus_tag="VFR00_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011470585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU0159318.1"
                     /translation="ILQKRGLQRATATDVMMPTAPEIGAEGRRGTAPAEEAYLRHVGE
                     RVRIGRARRGMSRKSLSQASGVSERYLAELERGAGNASLLVLRQIAEALGLEMASLVG
                     DRPELPLELRLAMHQLERLTPGQLAEARHLIQQRFEANGDTGDGRIALVGLRGAGKTT
                     LGRRAADELNMPFVELDREVERASGMELSEIFALHGQSMYRRLERQCLETVVARFQRV
                     MIATGGSLVTEPATYDLLLSTCFVVWLSATPDEHMSRVLAQGDLRPMADAPQAMEDLK
                     AILDSRTPLYAKANAELMTSGRSETQALSMLVAIISAAAPSQPTPPAGAHE"
     gene            1065..1997
                     /locus_tag="VFR00_08390"
     CDS             1065..1997
                     /locus_tag="VFR00_08390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015316608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HEU0159319.1"
                     /translation="MAKSRKVIITCAVTGSIHTPSMSPHLPITAEEIADAAVAAAEAG
                     AAVVHLHARNPRDGRPDQSPEAFQPFLKVIKQRSGCVINITTGGAPTMTIEERVRPAA
                     TFKPEIASLNMGTMNFGLFPMLQRQKEFKYEWEKPYLEASRAGMFKNTFADIEYILTT
                     CANNGSRFEIECYDIGHLYTLRHFADRGVVKPPFFIQSVFGILGGIGPHPDDVLHMKR
                     TADRLFGDQYRWSVLGAGRNQMPIAAMSLAMGGNVRVGMEDSLWIGPGQLARSNAEQV
                     KKVRQIIEGLGLDIASSDEARDILELKGGDKVAF"
BASE COUNT          413 a          701 c          729 g          372 t
ORIGIN      
        1 tatactgcag aaaagagggc tccagcgggc cacagcaacc gacgtgatga tgccaacagc
       61 acccgagatt ggcgcggaag ggcggcgcgg tacggcaccg gcggaggagg cctatctcag
      121 gcacgtcggc gagcgtgtgc ggatcggccg ggcccggcgc ggcatgtcgc gcaagagcct
      181 gtcccaggcc tccggggtgt cggagcgcta cctggccgaa ctcgagcgcg gcgccggcaa
      241 tgcctccctg ttggtgctgc gccagatcgc cgaggcgctc gggctggaaa tggcaagcct
      301 tgtcggggac cgcccggagc tgcccctcga gctgcggctc gccatgcacc aactggagcg
      361 gttgacgccg gggcagcttg ccgaagcccg gcacctgatc cagcagcgct tcgaggccaa
      421 cggcgatacc ggcgacgggc gcattgcgct ggtgggcctg cgcggcgccg gcaagaccac
      481 gcttgggcgc cgggcagccg atgaactcaa tatgcccttc gtcgagctcg accgcgaagt
      541 ggagcgggcg agcggcatgg agttgtccga gatttttgcg ctgcatggcc aaagcatgta
      601 tcgccgactg gagcgccaat gtttggaaac cgtggtggca cgcttccagc gcgtgatgat
      661 cgccacgggc ggcagcctgg tgaccgaacc ggccacctat gacctcttgc tctcgacgtg
      721 ttttgtcgtc tggctcagcg ccacgcccga tgaacacatg agccgggtgc tggcgcaggg
      781 ggacctgcgg ccgatggcgg atgcacccca ggcgatggaa gatttgaagg cgatcctgga
      841 cagccgcaca cccctttatg ccaaagccaa tgccgaactg atgacaagtg gcaggtcgga
      901 aacccaagcg ctcagcatgc tcgtggcgat catcagcgcc gccgcgccga gccagccaac
      961 gccgccggcc ggcgcgcacg agtagcttgc gcgcagcctc ggttccggct cagcatggca
     1021 tccggtcgcg tgcgacgcac cagtagcgca gaggagaccc cgcgatggcg aaatcccgta
     1081 aagtcattat cacctgcgcc gttaccggtt ccattcacac cccttccatg tctccgcatc
     1141 tgcccattac ggcggaggag atcgccgacg ctgccgtggc cgcggccgag gccggcgccg
     1201 ccgtcgtgca cctgcacgca cgcaatccca gggatggccg cccggatcaa tcccccgagg
     1261 cgttccagcc gtttttgaag gtgatcaagc aacgctcggg ctgcgtcatc aacatcacga
     1321 ccggcggtgc tcccaccatg accatcgagg agcgcgtgcg gccggcggcc accttcaagc
     1381 cggagattgc cagcctgaac atggggacca tgaacttcgg tctgttccct atgctgcaac
     1441 ggcagaagga gttcaaatac gaatgggaga agccgtatct ggaggcctcc agggccggca
     1501 tgttcaaaaa caccttcgcc gatatcgagt acattctcac cacctgcgcc aacaacggct
     1561 cgcgcttcga gatcgagtgc tacgacatcg gccatctcta taccttgcgc cactttgccg
     1621 accggggcgt ggtcaagccg ccgtttttca tccaatcggt gtttggcatt ttgggtggca
     1681 ttggcccgca tcccgatgac gtgctgcata tgaagcgcac ggccgatcgc ctgttcggcg
     1741 atcagtatcg ctggagcgtg ttgggtgccg gtcgcaatca gatgccgatt gccgccatgt
     1801 cgctcgccat gggcggcaac gtgcgcgtcg gcatggaaga ttcgttgtgg atcggcccag
     1861 gtcagcttgc gcgcagcaac gccgagcagg tgaagaaggt gcgccaaatc atcgaaggcc
     1921 tcggacttga tattgcgagc agcgacgagg ctcgcgacat tctggagctg aagggcgggg
     1981 ataaggtggc gttttgaggt ccgtgcccgc taagcgcccg aggagcgctc aaaggcgcgc
     2041 caggcggggt gggttagccg agctcgtcgg taacaaagcg gtatttgcgc gaacagaact
     2101 cgcacgtcac ggtgatgccg ccgtcggctt cgcgcatgtc cgctagctca tcgggcccga
     2161 aacgctgcag aaacatcttg atacgctcgc gcgagcagcg gcaggcggcg gccac
//
LOCUS       DASTMT010000360         3382 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_5025003, whole genome shotgun sequence.
ACCESSION   DASTMT010000360 DASTMT010000000
VERSION     DASTMT010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3382)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3382)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3382
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5025003"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1179
                     /locus_tag="VFR00_08395"
     CDS             <1..1179
                     /locus_tag="VFR00_08395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016846714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0159320.1"
                     /translation="QALIGSAASTATLASGVFRPAIAASEPIRIGYLPALTGPSSSTG
                     IGINRGTELAVKEINDAGGINGRQIELVVRDTQSDPTKAVNAATELTRRHKVHVIWGP
                     LNSGEALAATPLIARDGVPQLHPCWVDQLTDVKKYPLAFRNAPTNQQIGGAANHYVVN
                     IVKAKKVAVISDTTGYGTASLNAYVPMLKTLGAEVVYEGSVESNNPDLKPELLRMQAA
                     GAQAIMPWSVNAGFLSRIINTRAEMNWDIPIVGQTTLGSGQTKALLKKPEYWEKVYPN
                     NFRNCSYNTAGALPPRTVEFVERIRKANIEMSDTLLWWIALGYDGPRLMAEAIKTAGS
                     GPEEIAGYWNKVKAWPGVYGSISWTPELHDGFQNEEIVMCQANSLRDGAFTLAPGYTG
                     "
     gene            1184..2041
                     /locus_tag="VFR00_08400"
     CDS             1184..2041
                     /locus_tag="VFR00_08400"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEU0159321.1"
                     /translation="MLLSIVSYGLALGAVYALIGITYNVMFAASRVFSFTAGTVGMLG
                     GVFGALCIDRMGMPVALGFLVTLAAGAVLGVITELLTVRPVLKSLDQHLYVLSTLAFA
                     LMLQQLTAIEWSTEPQPFPRLLPFGPGFFDQQFWLPISACVLSIIGLEWLYRRTLVGR
                     AFLAISEDNYAARALGLPEQRLRIASYALAGAIGTLAGFTGGEMMLAYFGNGPLLNFY
                     GFVPVALGGMGSNRGAVAGGLALGLFQQTANFLVGGIFASVAIFVVFILVLLVRPEGF
                     AGAAPARRV"
     gene            2038..>3382
                     /locus_tag="VFR00_08405"
     CDS             2038..>3382
                     /locus_tag="VFR00_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016846716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU0159322.1"
                     /translation="MNDSATLLPAVPRVKPGRLEAWLPTAMLAVAGAALPLLGDSYLG
                     VIATRAAIYWVLVSGLNLVVGFAGQIAIGWVALLTLGAYVTSVLCAGTVTPELSPYLA
                     LLVAGLVGAIFGLIIGLPALRLRTFYFAMTTLGFATIVTQVALAWKSVTGGGVGVPGP
                     VLPWPFASPWGFYYFCLGLALLCTWMTTNIAASRFGRSLVALRDAEVAAEAVGISKAA
                     LLVTVFLFSGALAAIAGGLFASLQSYITPDAFTFDLSVLFFIAILIGGRGSILGPLLG
                     TIILTALPEFAAPLVAWSTFLYAVLLLVIVLAVPGGIADMLDFKNRRPLERHREIRPR
                     PELLAHIIPPSAQESLLKLENLSLSFGGVRAIDAVDLTLRTGEVHGLIGPNGSGKTTT
                     LNVISGYYPPQQGQVRLNDKVLAAGAAAARANQRIARTFQTPRLVGTASVLENVMI"
BASE COUNT          518 a         1122 c         1072 g          670 t
ORIGIN      
        1 caggctttga ttggatccgc ggcgagcacc gcgacgcttg ccagcggtgt gtttcggccc
       61 gccattgccg cctccgaacc catccgtatt ggctacctgc cggccctgac ggggcccagc
      121 tcttcgaccg ggatcggcat caaccgcggc accgaattgg cggtcaagga gatcaacgac
      181 gcgggcggca tcaacgggcg gcagatcgag ctcgtcgtgc gcgacacgca aagtgacccc
      241 accaaggcgg tcaacgcggc caccgaactt acgcggcggc acaaggtgca cgtgatctgg
      301 ggccccctga attccgggga ggcgctcgcg gcgacgccgc tcattgcccg cgacggggtc
      361 ccccagttgc acccgtgctg ggtcgatcag ctcaccgatg tgaagaaata cccgctggcg
      421 ttccgcaacg cccccaccaa tcagcaaatc ggcggcgccg ccaaccacta tgtggtgaat
      481 atcgtcaagg cgaagaaggt ggccgtcatc agcgatacca cgggctacgg cacggcgtcg
      541 ctcaacgcct acgtgccgat gctcaagacc ttgggtgccg aggtcgtcta tgaaggcagc
      601 gttgaatcga acaacccaga cttgaagccg gaattgctgc gcatgcaggc ggccggcgca
      661 caggcgatca tgccctggag cgtcaatgcc ggttttctct cgcgcatcat caacacgcgc
      721 gcggaaatga attgggacat tccgatcgtc gggcagacga cgctcggctc cggccagacc
      781 aaggccttgt tgaagaagcc cgaatactgg gaaaaggtct atccaaacaa cttccgcaac
      841 tgcagttaca ataccgccgg cgccctgccg ccgcgcacgg tcgagttcgt cgagcggatc
      901 cgcaaggcca atatcgagat gagcgatacg ctgctgtggt ggatcgcgct gggctacgac
      961 ggtccgcgct tgatggcgga ggccatcaag acagcgggca gcgggcccga ggagatcgcc
     1021 ggctattgga acaaggtcaa ggcctggccc ggggtctatg gcagcatcag ctggacgccg
     1081 gagttgcacg acggcttcca gaatgaagaa atcgtcatgt gccaagccaa ttcgctgagg
     1141 gatggggcct tcaccttggc gcccggctac accggataga tcgatgctgc tgtcgatcgt
     1201 ctcctacggc ttggcgctgg gagccgtcta tgcgctgatt ggcattacct acaatgtcat
     1261 gttcgcggcc tcgcgcgtgt tttcctttac cgcgggcacc gttggcatgc ttgggggcgt
     1321 cttcggcgcc ttgtgcatcg accgcatggg tatgccggtc gcgctcggtt tcctcgtcac
     1381 gctcgcggcc ggcgcggtgt tgggcgtcat caccgagctg ttgacggtgc ggccggtgct
     1441 gaaaagcctg gaccagcacc tttacgtgct ctccacgctt gcctttgccc tgatgctgca
     1501 acagctgacc gcgatcgagt ggagcacgga gcctcagccg tttccgcgcc tgctgccgtt
     1561 cggacccggc tttttcgacc agcaattctg gttgccgatc tcggcctgcg tgctgagcat
     1621 cattggcctc gagtggctct atcggcgcac gctcgtgggg cgcgccttcc tggccatctc
     1681 cgaggacaac tatgcggcac gcgcgctcgg cctacccgaa cagcgcctgc gcatcgccag
     1741 ctatgcgctc gccggcgcca tcggtacctt ggcgggcttt acgggaggcg agatgatgct
     1801 cgcctatttc ggcaacggcc cgctcctgaa tttttacggt tttgtccccg tcgccctcgg
     1861 cggcatgggc agcaatcgtg gtgcggtggc cggcgggctc gcgctcgggc tctttcagca
     1921 gacggcaaac ttcttggtgg gcgggatttt tgcctcggtt gccattttcg tcgtctttat
     1981 cctcgtcctg ctggtgcgcc ccgagggctt tgccggcgcc gcccccgccc ggcgcgtatg
     2041 aacgatagcg ccacattgct gcctgccgtg ccaagggtga agcccggccg cctcgaggcc
     2101 tggttaccca cggcgatgtt ggcggtcgcc ggcgcggcgc tgccgctgct cggggattcc
     2161 tacctgggcg tgatcgcaac gcgggccgcc atctactggg tgctcgtctc gggcctcaac
     2221 ctggtcgtgg gctttgccgg ccaaattgcc attggctggg tggcactctt gacgctcggc
     2281 gcctacgtca ccagcgtttt gtgcgccggc accgtgacgc cggagctgtc accttacctg
     2341 gcgttgctgg tcgccgggct ggtcggcgcg atcttcggcc tgatcatcgg cctgccggcg
     2401 ctgcggctgc gcaccttcta ctttgccatg accaccctgg gatttgcgac catcgtcacg
     2461 caggtcgcgc tggcctggaa gagcgtcacg ggcggcggcg tgggcgtgcc cggtccggtg
     2521 ctgccatggc ccttcgccag cccgtggggc ttttactatt tctgtctggg cttggcgctc
     2581 ttgtgcacct ggatgaccac gaacatcgcc gccagccgtt ttggtcgttc gctggtggcg
     2641 ctgcgcgacg cggaggtggc cgccgaggcc gtcggcatct ccaaggcggc gctgctggtc
     2701 accgtcttcc tgtttagcgg cgcgctcgct gccatcgccg ggggtctatt tgcctcgctg
     2761 caatcctaca tcacgcccga tgccttcacc ttcgacctct cggtgttgtt cttcatcgcc
     2821 atcttgatcg gcggccgagg ctcgatcctc ggtcccctgc tggggaccat cattctgacg
     2881 gcgttgcccg aatttgccgc gccgctggtg gcctggtcga cgtttcttta tgccgtcctg
     2941 ctgctcgtca tcgtgctcgc ggtccccggc ggcatcgccg acatgctcga cttcaagaac
     3001 cgccggccgc tcgagcggca tcgcgagatc cgcccgcgcc cggagctgtt ggcccacatc
     3061 ataccgccca gtgcacagga gagcctgctc aagctcgaga acctgtcctt gagttttggc
     3121 ggcgtgcgcg ccatcgacgc ggtcgatctc acgctccgca ccggcgaagt gcatgggctg
     3181 atcggcccca atggcagcgg caagaccacg acgctcaacg tgatctccgg ctactaccca
     3241 ccacagcagg ggcaggtgcg cttgaacgac aaggtgctcg ccgcgggcgc cgccgccgca
     3301 cgggcaaatc agcgcatagc gcgcaccttt caaacgccgc gcctggtcgg caccgcctcg
     3361 gtgctcgaaa acgtcatgat cg
//
LOCUS       DASTMT010000361         2672 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_3479067, whole genome shotgun sequence.
ACCESSION   DASTMT010000361 DASTMT010000000
VERSION     DASTMT010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2672)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2672
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3479067"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..122
                     /locus_tag="VFR00_08410"
     CDS             <1..122
                     /locus_tag="VFR00_08410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003574248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEU0159323.1"
                     /translation="ELAEILEQPVHLFLFVKVRQDWENDPERYRQMGLTFPKA"
     gene            147..872
                     /gene="recO"
                     /locus_tag="VFR00_08415"
     CDS             147..872
                     /gene="recO"
                     /locus_tag="VFR00_08415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEU0159324.1"
                     /translation="MEWSDEGIILSVRPHGETGAVLELFTRRNGRHLGLVHGGRSRKL
                     RPVLQIGTHVEASWKARIADNLGHFALELRKSYAALVLDEPAALAALTSIAALARLLP
                     ERDPHPNLFEVTLFVLGYLDEREVWPALLVRWELALLEELGFGLDLTSCAATGATTDL
                     TYVSPKSGRAVSSAAGEPYKERQLALPHFLRGRAADGVTAADLAAGLALTEHFLLARV
                     LRPRDLAMPEARNRLLSYLRPNV"
     gene            952..1581
                     /locus_tag="VFR00_08420"
     CDS             952..1581
                     /locus_tag="VFR00_08420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0159325.1"
                     /translation="MLWLFSWLAQGLENPVPSLKSWHDHPAPTLAALGLVSGILSALV
                     GFEVRIDALDWPGTVFFLSAEMMPIGLLFAAVMAFAIAAWHSPAWAPIVTFVGTLYAW
                     SAAIQTALFMHRIGGGDATHAGPLLAGVFAGFVGAALTHAAVALFAPELRDVTSATRT
                     CLVGTVAGLLFYFGSRNLIDARILFLVWQPAVAYSIGLGLAAKPAPQTL"
     gene            complement(1618..2286)
                     /locus_tag="VFR00_08425"
     CDS             complement(1618..2286)
                     /locus_tag="VFR00_08425"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HEU0159326.1"
                     /translation="MPVLPDLPQTELAIVEMTNAFRKEQRLGALTPNAALTAAARAFA
                     DYLGRTGKFAHEADGREPHQRAEAQGYRYCLVAENLAFNLDSRGFETRKLAFDVVEGW
                     KGSPGHRRNMLEKDATEIGVGVVRAKDRQPKFVSVQLFGRPESLKVSFAIENRTGAAV
                     TYRLGDDSETLPARATASHTSCTAEPLRIDKAGPAAAALQPRDGDTFVLTPGAGGALR
                     LERK"
BASE COUNT          391 a          941 c          875 g          465 t
ORIGIN      
        1 gcgagctcgc cgaaatcctc gagcaacccg tgcatctgtt cctgttcgtc aaggtgcgcc
       61 aagattggga aaacgatccg gaacgctacc ggcagatggg gctgacgttt cccaaagcct
      121 aaccgtcgcc ggcaggacgc cccgccatgg aatggtcgga tgaaggcata atcctcagcg
      181 tgcggccgca cggtgagacc ggggccgtgc tcgagctctt cacgcgccgc aacggccgtc
      241 acctgggcct ggtgcacggc ggccgctcgc gcaagctgcg cccagtactg cagatcggca
      301 cgcacgtgga ggcgagctgg aaggcgcgca ttgccgacaa cctcggacac ttcgccctgg
      361 agttgcgcaa aagctatgcg gcgctggtgc tcgacgagcc cgccgccctc gccgccttga
      421 cctccatcgc cgcgctggcg cgcctgctgc ccgaacgcga tccccacccc aacctgttcg
      481 aagtcaccct gttcgtgttg ggctacctcg atgaacggga ggtgtggccg gcgctattgg
      541 tgcgctggga gctggcgctg ctggaggaac tggggttcgg cctcgatctg acgagctgtg
      601 ccgccacggg ggccacaacc gacctcacct acgtctcgcc caagtcgggg cgcgcggttt
      661 ccagtgctgc gggcgagccc tacaaggagc ggcagctggc gctgccgcat ttcctgcgcg
      721 gtcgcgccgc cgatggcgtt acggccgccg acctggccgc cggcctggcg ctcaccgagc
      781 attttttgct ggcgcgcgtg ctgcgccccc gcgacctcgc catgcccgaa gccaggaacc
      841 gcctgttgtc ttaccttcgg ccgaatgtgt agctatcgag gccgcgtgca cgggctcctt
      901 ggcttggccg gtcccgttgg cgcctatggt ttggttgtgt ggtctggttg tgtgctttgg
      961 ttgttcagtt ggctcgcgca aggactagaa aaccccgtgc cctcgctcaa atcatggcat
     1021 gaccacccgg cccccacctt ggcggcgctc ggcctcgttt cgggtatcct gtccgccctc
     1081 gtcggctttg aggtgcgcat cgacgccctc gactggccgg ggaccgtgtt cttcctgtcc
     1141 gccgaaatga tgccgatcgg cctcttgttc gccgccgtga tggccttcgc catcgccgcc
     1201 tggcacagcc ccgcctgggc gccgatcgtc accttcgtgg gcacgcttta tgcctggagc
     1261 gcggccatcc aaaccgcgct ctttatgcac aggatcggcg gcggcgatgc cacccacgcc
     1321 gggccgctgc tggccggcgt gttcgcgggt ttcgtcggcg ccgccctcac gcacgcggca
     1381 gtggccctgt tcgcgcccga gctgcgcgat gtcacgtcgg ccacgcgcac ctgcctggtc
     1441 ggcaccgtcg ccggcctgct gttctatttc ggcagccgca atctgatcga cgcgcgcatt
     1501 ctgtttctgg tctggcagcc ggcggttgcc tactcaatcg gcctcgggct ggcggccaag
     1561 cccgcgccgc agaccttgtg aagcaccggg ccgcagcggc gaatgctgcc ggcggcgcta
     1621 cttgcgctcg agcctgaggg cgccgccggc gcccggcgtc agcacgaagg tgtcgccgtc
     1681 gcgcggctgc agagccgccg ccgccggtcc cgccttgtcg atccgcaagg gctcggcggt
     1741 gcagctggtg tggctcgccg tcgcccgcgc cggcagcgtt tcgctgtcat cgcccagcct
     1801 gtaggtgact gccgcccccg tgcggttctc gatggcgaag ctcaccttca gcgactcagg
     1861 ccgaccgaac agctgcacgg agacgaattt tggctgacgg tccttcgccc gcaccacgcc
     1921 gacgccgatt tcggtggcgt ccttttccag catgttgcgg cgatggcccg gcgagccctt
     1981 ccagccctcg accacatcaa aggcgagctt gcgcgtctca aagccgcgac tgtcgaggtt
     2041 gaaggccagg ttttccgcga ccagacagta gcgatagccc tgcgcctcgg cgcgctggtg
     2101 tggctcgcgg ccgtcggcct catgcgcgaa cttgccggtt ctgcccagat agtccgcaaa
     2161 ggcgcgcgcc gccgcggtca gcgccgcatt gggtgtcagc gcccccaaac gctgctcttt
     2221 gcgaaaggcg ttcgtcatct cgacgatggc aagctcggtc tggggcaggt cgggaagcac
     2281 gggcattaac agcgttccgg cagcggcgcg cgccaccgcg gcgcagcacc aacaagacgc
     2341 tgacgggaca ggctctgagc ttggagccta tccgccgggc ggggcaggcg tttttccgcg
     2401 ccaatgaggg catgcggcaa ggccgcgcgc aagaggcacc gaggcgccat ccacgaccaa
     2461 acggagggac accaaagggg gcgaccaagg ggggcccgac ggggggagaa gaacccaggc
     2521 gccgtcgctt gagccggccc gcgtcctggg ctaacccaag gtccatgagc aagaccacga
     2581 ttctcgcccc gccgccgccc atcgagccca tcaaactcaa ggatgcgctg gaggagcgct
     2641 atctcgccta cgccctctcc accatcatgc ac
//
LOCUS       DASTMT010000362         5069 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_5352039, whole genome shotgun sequence.
ACCESSION   DASTMT010000362 DASTMT010000000
VERSION     DASTMT010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 5069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5069
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5352039"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1027)
                     /gene="meaB"
                     /locus_tag="VFR00_08430"
     CDS             complement(<1..1027)
                     /gene="meaB"
                     /locus_tag="VFR00_08430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013947086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HEU0159327.1"
                     /translation="MPLPAGKAKALRIAAAAAPGYQALMPPKPKTSKSVASPDLAQLV
                     SRMRSGDRRATSRVVTELERLSPELPALMQELNPHLGHALVVGFTGPPGAGKSTLVNA
                     YTAHLRQAGKSVGIIAVDPSSPVSGGAILGDRIRMPATLSDDGVFMRSLASRGHLGGL
                     CPAAVRIIDGLDAAGKDTILLETVGTGQSEIDVAEVADVRVVVMAPGLGDDIQAMKAG
                     LLEIADIVVVNKADREGAERTLNQLNGAAQLRATRSKATVLKTTATSGDGIATLADRV
                     AELAAARLAVEARERRRRRARYLIARAAADLIAERIRGGRQSGLDRLSDAVLTGELLP
                     ADAARKLV"
     gene            complement(1032..2402)
                     /locus_tag="VFR00_08435"
     CDS             complement(1032..2402)
                     /locus_tag="VFR00_08435"
                     /inference="COORDINATES: protein motif:HMM:NF042645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6600 domain-containing protein"
                     /protein_id="HEU0159328.1"
                     /translation="MTAVALTLSKARSCLVLAALLAILALLCAPAMAQDQGTAQDRGV
                     DIDVFYEQLAPYGQWFEHPVWGTVWRPRVDQDWRPYAQGAWVYTDDFGWYWEAEEPWG
                     WAPFHYGRWLIDEDGSWIWIPDTEWGPAWVAWRTSEEYVGWAPLPPDARWGPDGELIF
                     DAEFYSAPRYHLAWCFVRPHQLILPGLHRYLLPRRYASSIYRQSRPLRAHQRVEGRFI
                     HAGFDVRRFERMTGRPVVRVRLKSVDNPQAVSALRGSQRGAELSTFKPRFIDRPEGSG
                     RIHRPAAADPGRFDARGPSAEREQRWRGGPGGALPPPAATLGRPNTEPSSGPTGNAEP
                     TPPGARVLNRDTRERRPDFGPRDSGTTQRPRSPTTPYGEADPGRGPPPGLQQRPYGAP
                     PGTPRAYSAPQPTARPIPPATGQVAPPTNLRAPPPGPLPPARSGPGPGEERRAPQRKD
                     ENKGPG"
     gene            complement(2498..4288)
                     /locus_tag="VFR00_08440"
     CDS             complement(2498..4288)
                     /locus_tag="VFR00_08440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013947563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEU0159329.1"
                     /translation="MTYAKVLLTLLLLASWPFTATGAAPVHGIAMHGTPKHAPGFTHF
                     PYVNPDAPKGGRLVLGTLGTFDSLTPFIIKGVSPSSMREYVYESLLTRSGDEAFSLYG
                     LIAESIELPEDRSHITFHLRPQARFSDGTPITPEDVLFSHEVLKQKGWPYHRSHYAKV
                     ARAEKISDHAVRFTFDAAGDREIPMIIGLMPILPKHKTSLENFETTTLEPPIGSGAYT
                     VARVDPGRSLTYKRNPDWWGGDLPVTRGRFNFEEIRVEYFRDAASLFEAFKAGEIDMR
                     IEEDPGRWIEGYRFPAVNEGRVIKREFETGLPAGMSALVFNTRRAVFRDARVRRAFIL
                     LFDAEWINRNLYNGLFNRTQSFFERSELASSGRPADARERALLAPYLGELKPEILDGS
                     YRFPSNDGSGDNRANMAQAFKLLSEAGYRLENNTLSKDGVPLSFEFLAQTRQQERLML
                     SYARTLERLGIAVRIRQVDTSQYWARLKSYDFDLIQWSWSASLSPGNEQINRWSSKAA
                     RIEGTLNYPGVENPAVDAMIDALLQAEAADDFVAAVRALDRALLSGDYVIPLFFLPRV
                     WVAYWSYLKFPAVQPLAGVDIDTWWSERAN"
     gene            4585..>5069
                     /locus_tag="VFR00_08445"
     CDS             4585..>5069
                     /locus_tag="VFR00_08445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0159330.1"
                     /translation="MRQFIFPSTTLGQVRTAGALAAALMLGITVLLAPAGAQQPPAKK
                     DNKAPATPAAPPAQKPAAPAAAAPGKAAPATAAAGNQSAWVKLCEKATATQKTKDGKD
                     EKKDLNICLTHHERLDGNSGMVLVSAAVRQVEGQDKQAFMVMVPLGMMLQAGMRATVY
                     P"
BASE COUNT          859 a         1634 c         1770 g          806 t
ORIGIN      
        1 ccaccagctt ccgggcggcg tcggcgggca acagctctcc tgtcagcacg gcatcgctca
       61 gacgatcgag cccactctgg cggccgccac ggatgcgctc ggcgatgagg tcggcggcag
      121 cccgcgcaat cagatagcgc gcgcggcggc ggcgccgttc ccgcgcctcc acggcaagcc
      181 gtgccgcggc gagttcggcc acccgatcgg ccaaggtggc gatcccgtcg ccgctggtgg
      241 cggtggtctt gagaacggtt gccttggacc tggtggctct cagctgcgcc gccccgttca
      301 attggttgag cgtgcgttcg gcgccctccc ggtcggcttt attgacgacc acaatgtcgg
      361 cgatctcgag caggccggct ttcatggctt gaatatcgtc gccaagaccc ggcgccatta
      421 ccaccacgcg cacgtcggcg acctcggcca cgtcgatctc gctctgcccg gtgcccacgg
      481 tctccaacag aatggtgtct tttcccgccg catcgaggcc atcgatgatg cgcaccgcgg
      541 ccgggcatag gccacccaaa tgcccgcggc ttgccaacga gcgcataaag acgccgtcat
      601 cgctaagcgt ggccggcatg cgaatacggt ccccgagaat ggcacccccg gacaccgggc
      661 tcgacgggtc gacggcaatg atgccaacac ttttgccggc ttggcgcagg tgggccgtgt
      721 aggcgttcac aagggtcgac ttgcccgcac caggcgggcc cgtgaagccg acgacgaggg
      781 cgtgacccag gtgcgggttc agctcctgca tcagcgcggg cagctcgggt gacaggcgct
      841 ccagctcggt gaccacccgc gaggtggcgc gtcgatcgcc gctgcgcatg cgcgaaacga
      901 gctgggcgag atcgggtgag gcgacgcttt tgctggtttt gggtttgggc ggcatcagcg
      961 cctgatagcc gggcgcggcc gcggccgcaa tgcgcagcgc tttcgccttg cccgctggca
     1021 acggcaagcg ctcagcccgg tcccttattc tcgtccttgc gctgcggcgc gcggcgctcc
     1081 tcgccgggcc cggggccact gcgtgccggg ggcaagggcc ctggcggcgg cgcgcgcagg
     1141 ttggttggcg gggcgacctg ccccgtggcc ggggggatcg gccgtgccgt gggttggggg
     1201 gcgctatagg cccgcggcgt tcctgggggg gccccgtagg gtcgttgctg caggccgggg
     1261 ggcggtcccc tgcccggatc tgcctcgcca tagggcgtcg tgggagagcg cgggcgctgc
     1321 gtggtgccac tgtcccgcgg cccgaaatcc ggccggcgct cgcgcgtatc ccggttcagg
     1381 acgcgcgccc cgggcggggt gggctcggca ttgccggtgg gcccggagga gggttcggtg
     1441 ttgggacggc cgagcgttgc tgccggcggc ggcagcgcac caccagggcc gccgcgccag
     1501 cgctgctcgc gctccgcgct cggcccccga gcgtcgaaac ggcccgggtc cgccgcggcc
     1561 gggcggtgaa tacgcccgct cccctcgggg cgatcgataa agcgcggctt aaaggtcgaa
     1621 agctcggcgc cgcgctgaga accgcgcagg gcggacacgg cttgaggatt gtccacgctt
     1681 ttaaggcgca cgcgtacgac cgggcggccg gtcatgcgct cgaagcggcg cacgtcgaac
     1741 ccggcatgga taaaccgccc ctcgacgcgc tggtgtgccc gcaacgggcg cgactggcgg
     1801 tagatcgagg aggcgtagcg gcgcggcagg agatagcggt gcagccctgg caggatcagc
     1861 tgatgcgggc gcacgaagca ccacgccaaa tggtagcgcg gggcgctata aaactcggca
     1921 tcgaagatga gttcgccgtc cgggccccag cgcgcgtccg gcggcagcgg cgcccagccg
     1981 acgtattcct cgctcgtgcg ccaggccacc caagccggac cccattccgt gtcgggtatc
     2041 caaatccaag acccgtcttc gtcgatcagc cagcgtccgt agtgaaaggg cgcccagccc
     2101 cagggctcct cggcctccca gtaccagccg aagtcgtcgg tatagaccca ggcgccctgc
     2161 gcgtaggggc gccaatcctg gtcgaccctg ggccgccaga ccgtgcccca gaccggatgc
     2221 tcgaaccact gcccgtaggg cgcgagctgt tcgtaaaaga cgtcgatatc gacgccgcgg
     2281 tcctgcgccg tgccttggtc ctgcgccatg gcgggcgcgc agagaagtgc caggatggcg
     2341 agcagcgccg ccagcacgag gcaagagcga gcttttgaca gggtcagcgc caccgcagtc
     2401 accgtcgttc ctcccctttg tttggcctcg ccttggtgcc gaacgaatgc gttgcggaca
     2461 tggcgtcggc ctgaataggt ggtgaacggg cgcgggccta gtttgcccgc tccgaccacc
     2521 aggtgtcgat atcgacgccc gccaacggct gcacggcggg aaacttcaaa tacgaccagt
     2581 aggccaccca gacgcgcggc agaaagaaga gcggaatgac gtagtcgccg gacagcaagg
     2641 cgcggtcgag cgcgcggacg gcggcgacga agtcgtctgc ggcttccgcc tgcagcaggg
     2701 catcgatcat ggcatcgacg gcgggatttt ccacgcccgg atagttgagc gttccctcga
     2761 tgcgcgcggc cttgctgctc cagcggttga tctgctcgtt gcccggcgaa agcgaggcac
     2821 tccagctcca ctggatcaaa tcgaagtcgt agctcttgag gcgcgcccag tattgcgagg
     2881 tgtcgacctg acggatacgc acggcaattc caagccgttc gagggtacgc gcatagctca
     2941 gcatcagccg ttcctgctgg cgcgtctgcg ccaggaactc gaagctgagc gggaccccat
     3001 ctttgctcaa ggtgttattt tccaggcgat accccgcctc gctcaggagc ttgaatgcct
     3061 gagccatgtt ggcgcggttg tcgccgctac catcgttgga ggggaaacga tagctgccat
     3121 cgagaatttc gggtttcagc tcccccagat agggtgccag cagcgcgcgc tcgcgcgcat
     3181 cagccggccg gccgctcgag gcgagttcgg agcgctcaaa aaagctctgc gtccggttga
     3241 agagcccgtt gtagagattg cgattgatcc actcggcatc gaacagaaga ataaaggcgc
     3301 ggcgcacgcg cgcatcgcgg aacacggcac ggcgcgtatt gaagaccagc gccgacatgc
     3361 ccgcgggcag tccggtctcg aattcgcgct tgatcacccg gccctcgttc accgccggaa
     3421 agcgatagcc ttcgatccag cggccgggat cctcctcgat gcgcatgtcg atctcgccgg
     3481 ccttgaaggc ttcgaacagg gaggcggcgt cgcggaagta ctcgacgcgg atctcctcga
     3541 aattgaagcg gccgcgcgtc accggcaggt cgcctcccca ccaatcgggg ttgcgcttat
     3601 aggtgagcga acgcccagga tcgacgcgag ccaccgtata ggcgccactg ccgatcggcg
     3661 gctccagcgt cgtggtctcg aagttttcga ggctcgtctt gtgtttgggc aggatcggca
     3721 tcagcccgat gatcatgggg atctcgcggt cgccggcggc atcgaacgtg aagcgcacgg
     3781 cgtggtctga gatcttctcg gcgcgcgcaa ccttggcgta atgcgagcga tgatagggcc
     3841 agcccttttg cttcaggacc tcatgactga aaaggacgtc ctccggcgtg atgggcgtgc
     3901 catcggagaa gcgcgcttgc gggcgcaagt ggaacgtaat gtgcgatcgg tcttcgggta
     3961 gctcgatcga ctccgcgatc aagccataaa gggagaaggc ttcatcgccg ctgcgcgtga
     4021 gcaggctctc atagacgtat tcgcgcatgc tcgacggcga gacgcccttg atgatgaaag
     4081 gtgtcaggct gtcgaaagtg ccgagcgtgc ccaacaccag gcgcccgccc ttgggtgcgt
     4141 cgggattgac gtagggaaaa tgcgtaaagc ccggcgcgtg cttgggcgtg ccgtgcatgg
     4201 cgatgccgtg cacgggggcc gcgcccgttg cggtgaaggg ccaactcgcc agcagcaata
     4261 gggtcaggag gactttggcg taggtcattt gggcaggaga tggaggggag ggtgcgcccg
     4321 caccagggtg ggcgcgacgg cagcggcatt ctagcacaag ctcggcgcag caaggcttta
     4381 ccggcaaagg ggtctgcgtg tccgcgtcgc cacgcatccc ccggcaaggc ccaagctcag
     4441 gccgaaagcc tggggctgcg gtcacgggat tgccgcaatc cgaaccttac tgccacctgg
     4501 gcgcggatca ggcgcagcag ctgtaacgag ggtggggcca agccgccggt caatgccggg
     4561 ggtgcaggag aggtcaaaag cgacatgagg caattcattt ttccgtctac gacattgggc
     4621 caggtgcgca cagccggcgc gttggcggca gccctgatgc tgggaatcac cgtgctgttg
     4681 gccccggcag gggcgcagca gccgccggcg aagaaggaca acaaggcccc ggcgaccccc
     4741 gccgcgcctc cggcgcaaaa gccagcggcg ccggcggcag ccgcgccggg gaaggcggcc
     4801 cccgccactg cggccgcagg caatcagagt gcctgggtca agctatgcga gaaggcgacg
     4861 gcgacgcaga agaccaagga cggcaaggac gagaagaagg atctcaatat ttgtctgacc
     4921 caccacgaac gcctcgacgg caattcgggg atggtgttgg tctcggccgc cgtacgccag
     4981 gtcgaggggc aggacaagca agccttcatg gtgatggtgc cgctcggcat gatgctgcag
     5041 gcgggcatgc gcgccaccgt ctaccccaa
//
LOCUS       DASTMT010000363         3141 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_6008404, whole genome shotgun sequence.
ACCESSION   DASTMT010000363 DASTMT010000000
VERSION     DASTMT010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3141
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6008404"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..488)
                     /gene="dctP"
                     /locus_tag="VFR00_08450"
     CDS             complement(<1..488)
                     /gene="dctP"
                     /locus_tag="VFR00_08450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019404830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HEU0159331.1"
                     /translation="MRSAKYLLLAAALLVSGAPAALAQTKLKWAHVYETAEPYHTQAL
                     WAAEEIKKRTEGRYQIDVFPASQLGNENQINEGLGLGTVDMIYTGVAFAGSIHKPVAI
                     TNAPYVLRDFEHWKVYRDSALFRDIAKGYEDKTKHKVMSLTYYGQRMVTANKEIKKPE
                     DM"
     gene            complement(585..1286)
                     /locus_tag="VFR00_08455"
     CDS             complement(585..1286)
                     /locus_tag="VFR00_08455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013947587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HEU0159332.1"
                     /translation="MPHRPYRYFDLLMVGFVVVLLCSNLIGPAKIAVVDLPGWGPFAF
                     GAAAIFFPISYVFGDILTEVYGYAQARKVIWAGFAALLFASFMAAVVVALPPAPFWPH
                     QGAFEVAFGNTWRIVAASIIAYFCGEFVNAYVLAKMKVWTQGRALWTRTIGSTICGEA
                     VDSALFYPLAFYGTGIIPNAALPEVMLAQFALKTLVEVVFTPVTYAVVGTLKRAEGID
                     PFDRDTDFNPFKIAG"
     gene            1434..1643
                     /locus_tag="VFR00_08460"
                     /pseudo
     CDS             1434..1643
                     /locus_tag="VFR00_08460"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            1686..2153
                     /gene="queF"
                     /locus_tag="VFR00_08465"
     CDS             1686..2153
                     /gene="queF"
                     /locus_tag="VFR00_08465"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013947584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HEU0159333.1"
                     /translation="MAATVDREGLTALGQKAPTPLSPATALLERVANPHPDLNYLARF
                     TAPEFTSLCPVTGQPDFAHLVIDYAPQRWLVESKSLKLYLASFRNHGAFHEDCTLAIG
                     KRLVALLEPHYFRIGGYWYPRGGIPIDVFWQTGTLPANLWLPEQGVAPYRGRG"
     gene            complement(2162..2962)
                     /locus_tag="VFR00_08470"
     CDS             complement(2162..2962)
                     /locus_tag="VFR00_08470"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018063325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEU0159334.1"
                     /translation="MEFKGKVALITGAGNGIGRAAALGFAARGAEVVAVDLDGAAAER
                     TAATIQQQGGAAVPYRADVTKSSDVAAYVKAALDAYGKIDCFFNNAGIEGKVAPTAEY
                     DEAVFDQVIAVNVKGVFLGLRHVLPVLLKQKAGAIVNTASVAGLVATPGMSAYVASKH
                     AVLGLTKAAAGEVARSGVRVNAVCPGPIDTRMIHSLENQLNPGNPASVGDRYRASLPL
                     GRYGTAEEVANVVMFLSSDLAGNITGAHYVIDGGRSATGGSVTAQKQD"
BASE COUNT          572 a         1019 c          995 g          555 t
ORIGIN      
        1 ttcatgtcct cgggcttctt gatctccttg ttggcggtaa ccatgcgctg gccatagtag
       61 gtgagcgaca tcaccttgtg cttggtcttg tcctcgtagc ctttggcgat gtcgcggaac
      121 agcgcggagt cccgatagac cttccagtgc tcgaaatcgc gcagcacata aggagcattg
      181 gtgatggcaa ccggcttgtg gatcgagccg gcaaaggcca caccggtgta gatcatatcg
      241 acggtgccga gccccaggcc ttcattgatt tggttctcgt tgccgagctg ggaggcggga
      301 aaaacgtcga tctggtaccg gccctcggtg cgcttcttga tctcttcggc ggcccacaag
      361 gcttgcgtgt ggtagggttc agccgtctcg tagacgtgcg cccatttcag tttggtttgg
      421 gctagcgccg ctggcgcgcc gctcaccagc agcgccgccg ccagcaacag gtatttggca
      481 gatctcatgg gtcgttgtcc tcctccgcac aggcgcgaaa gcgcgcgcgc caagttatcg
      541 tataagcaga cgacctggcg tggtacggga tttttggccc cgcttcaacc cgcgatcttg
      601 aacgggttga agtcggtgtc gcgatcgaaa ggatcgatgc cttcggcgcg cttgagggtg
      661 cccaccacgg cgtaggtgac cggcgtgaac accacctcca ccagggtttt caaggcgaac
      721 tgcgccagca tcacctccgg cagcgccgcg ttgggaataa tgcccgtgcc gtaaaaagcc
      781 aagggataga aaagggcgct gtcgacggcc tccccgcaaa tcgtcgagcc gatggtgcgc
      841 gtccagagtg cacgcccttg ggtccacact ttcattttgg cgagcacata ggcgttgacg
      901 aactcgccgc agaagtaggc gatgatggag gcggcaacga tgcgccatgt gttgccgaag
      961 gcgacctcga aggcgccctg atgcggccag aacggtgccg gcggcaaggc caccaccacg
     1021 gcggccatga acgaggcaaa caggagcgcg gcaaagcccg cccagatcac cttgcgcgct
     1081 tgggcatagc cgtagacctc cgtcaggatg tcgccgaaca cgtaggaaat cggaaagaaa
     1141 atggcggccg ccccgaaggc gaagggcccc cagcccggca ggtcgacgac ggcaatcttg
     1201 gcggggccga tcaggttgga gcacagcagc accacgacaa agccgaccat cagcaggtcg
     1261 aagtagcgat agggtcgatg cggcatgggc gcgggccccc aaggctgttg aacggtcctt
     1321 ggcttagcaa agggcgatcg attgtctaag aaaaattgtc ggtctagtct tagtctagta
     1381 atacaagaca gagcgccttc cgtagaaccg tgcgcttccc gtagagagga ttgatggtcg
     1441 ccaaccttcg cccgggcctc gccccccttg gcaaacgcat tcagcaggcc cgccgggtag
     1501 ccggcctgac ccagccgcag gtcggccgcg cgctcggcgt cagcaaacag caggtcagcc
     1561 attgggagac cggacgctcc gacatcacgc gcgagcatct tctgcacttg gccaaactct
     1621 tcacggtgga gatggattgg ctggcgcgcg gtaccttgat cgaggtcccc ggcgccgtga
     1681 gcgcgatggc ggcaaccgtc gatcgtgagg gcctaaccgc gctgggccag aaggcgccga
     1741 cgccgctctc gccggctacg gcgttgctcg agcgcgtcgc caatccgcac cccgatctca
     1801 attatctcgc gcgcttcacg gccccggaat tcacctcgct ctgcccggtg accggccagc
     1861 ccgactttgc ccatctcgtg atcgattacg cgcctcagcg ctggctggtg gaatcgaagt
     1921 ccctgaaact ctatctcgcc tccttccgca accacggcgc cttccacgag gactgcacgc
     1981 tggcgatcgg caagcgcctc gtcgccctgc tcgagccgca ttattttcgc atcggcggct
     2041 actggtatcc gcgcggcggc atcccgatcg acgtcttctg gcagacgggc accctgccgg
     2101 cgaacctatg gctgccggag caaggggtgg ccccttaccg cggccgcggc tgaggcatcc
     2161 ctcaatcctg cttctgcgcc gtcaccgagc cgccggtggc gctgcggccg ccgtcgatca
     2221 catagtgcgc gccggtgatg ttgccggcga gatcggacga caaaaacatc accacattgg
     2281 cgacctcctc cgcggtgcca tagcgcccga gcggcaggct cgcccggtag cggtcgccca
     2341 cgctggcggg attgccgggg ttgagctggt tttccagcga atggatcatg cgcgtgtcga
     2401 tcgggcccgg acagaccgcg ttgacgcgca cgccgctgcg cgccacctcg ccggccgccg
     2461 ctttggtgag accgagcacg gcgtgcttgg aggcgacata ggccgacatg cccggcgtgg
     2521 cgacgaggcc cgcgacggag gcggtgttga cgatggcgcc cgccttttgc ttgaggagca
     2581 cgggaagcac gtgccgcagg cccaggaaca cgcccttgac gttgacggcg atcacctggt
     2641 cgaacacggc ctcgtcgtat tcggcggtgg gagcaacctt gccttcgatg ccggcattgt
     2701 tgaaaaagca gtcgatctta ccgtaggcgt cgagcgcagc cttgacgtag gccgcaacgt
     2761 cgctggattt ggtcacatcg gcgcgatagg gcaccgcggc gccgccttgc tgctggatcg
     2821 tcgcggcggt gcgttcggca gccgccccgt cgagatcgac ggccaccacc tcagcgccgc
     2881 gcgcggcaaa gccaagggcc gcggcccgac cgatgccatt gccggcaccc gtgatgagtg
     2941 cgaccttgcc cttgaactcc atagctgcgc ctccgagcct gttgtcgctg gcgaggatag
     3001 catgcggcat ccgcgtggcc agcccgcaaa cgcgcgggct tgctcgccgc caagaaggct
     3061 gaaaaactcg agccagctct tcacatcaca cccccccacg aaccatgcat gcttcgacac
     3121 ctgccgaaac aatcggccat t
//
LOCUS       DASTMT010000364         2467 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_6336543, whole genome shotgun sequence.
ACCESSION   DASTMT010000364 DASTMT010000000
VERSION     DASTMT010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2467)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2467)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2467
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6336543"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..211)
                     /locus_tag="VFR00_08475"
     CDS             complement(<1..211)
                     /locus_tag="VFR00_08475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0159335.1"
                     /translation="MPASANVPAGRVVVGAEVLAANGFAALAGKRVGLIANQTSLVRG
                     SHLADLLRAVPGLTLAAIYAPEHGFR"
     gene            complement(373..1287)
                     /locus_tag="VFR00_08480"
     CDS             complement(373..1287)
                     /locus_tag="VFR00_08480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HEU0159336.1"
                     /translation="MVSPTSSPPAGRRPPALRRCWLCLPGADQSALSAAMACGADVLI
                     QELEDFTPPEQRPGARALAADLFAQWRAAGIVAAVRINPLHTEGRADLEAVMRGRPDI
                     VLMSKVAEVDEVVALDGEVTRLEQALAIPTGSTELVPNVESARGIRHAYAIAKASPRI
                     TGVMGSTEDLAADLGAPRSKTGSELAYARQRLHFECTAANVLSIDCPYTFADLAGAAS
                     DARYARQLGYVAKSAVDPQHVAAINRVMTPSTDEIAEAHAVIAAFEAARAAGKERGRQ
                     GDLLVEVPTYFSAKRLIARAAALGVAGA"
     gene            1546..2178
                     /locus_tag="VFR00_08485"
     CDS             1546..2178
                     /locus_tag="VFR00_08485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009489697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU0159337.1"
                     /translation="MTRILIADDHEVVRSGLRKILESQPNWEVVAEVGDGKDAISKAT
                     ETRPDVAVLDYSLPLINGIEATRQIRARLPKTEVLIFTMHDNETLISELLKAGARGYL
                     LKSDAQSHLIGAIECLALHKPFFTAKVSEALLDSFLKRPAREGSMLTNRERCVVQLIA
                     EGHTNKQVATILSISLKTVETHRSAIMRKLNLSSSAGLVRYAIRNNIVQA"
     gene            complement(2288..>2467)
                     /locus_tag="VFR00_08490"
     CDS             complement(2288..>2467)
                     /locus_tag="VFR00_08490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018453211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0159338.1"
                     /translation="ATFPKAVSPKYTKESAGRARMHTCLDQYNANKATNGNGGMRWIM
                     KGGGYYSECNKRLKG"
BASE COUNT          444 a          791 c          809 g          423 t
ORIGIN      
        1 cgcggaagcc gtgctcgggg gcataaatcg cagccagggt gaggcccggt accgctcgca
       61 gcaggtcggc aaggtgggat cccctaacga ggctggtctg gttggcaata aggccgaccc
      121 gcttgcccgc caaggcggcg aagccgttgg ccgcgaggac ctcggcgccg acgaccacgc
      181 gtccggcggg aacatttgcg ctggcaggca tgcttgcatg tcctacgaca acggcgaggc
      241 ccaagcccag cgccaagcgt accaagcgaa atttaaggcc agcgtgcgag cgtgattggc
      301 gccaagcggc atgagggccc gtccgacctg cgcagcggcc gtataagcgc cagcgaagag
      361 ctttccgcag cattaggcgc ctgcaacgcc aagggctgcg gcacgcgcga tgagccgctt
      421 ggcactgaag taggtcggca cctcgaccag gagatcgcct tggcgcccgc gctccttgcc
      481 cgcggcgcgg gccgcctcga aggcggcgat gacggcgtgg gcttcggcga tctcgtccgt
      541 gctgggtgtc atgacccgat tgatcgcggc gacgtgttgg ggatcgaccg cgctcttggc
      601 cacatagccc agttggcgcg catagcgggc gtcgcttgcc gcacccgcaa gatcggcaaa
      661 agtataggga cagtcgatcg acagcacgtt ggccgcggtg cactcaaagt gcaggcgctg
      721 gcgcgcgtag gcgagttcgc tgcccgtctt gctgcgcggg gctccgagat cggctgccag
      781 atcctcggtc gaccccatca cgcccgtgat gcgcgggctc gccttggcga tggcataggc
      841 gtggcgaata ccgcgggccg attccacatt ggggacgagc tcggtgctgc cagtgggaat
      901 ggcgagcgcc tgctcgagcc gcgtcacctc gccatcgagc gccacgacct cgtcgacctc
      961 ggccaccttg gacatcagca cgatatcggg ccggccgcgc atgacggcct cgaggtcggc
     1021 acgcccctcg gtgtgcaagg gattgatgcg gaccgcggca acgatgccgg cggcccgcca
     1081 ctgggcaaag agatcggcgg cgagggcccg ggcgcccggg cgctgttcgg gcggcgtgaa
     1141 atcctcaagt tcctggatca aaacgtcggc gccgcaggcc attgccgcgc tgagcgcgct
     1201 ctgatccgcg cccggcaggc aaagccagca gcggcgcagg gcgggcgggc ggcgcccggc
     1261 cggaggcgat gaggtgggag ataccataag tatttgaacg cgggccgcgt tctccccgtg
     1321 gttttgctgg cctgtcagtc tttgggcggc acagtctggc gccctgtgga aggcttatac
     1381 gcggcccgcg cgctacccga aaaggggagg gcatcccatc aggctgcgga tgatcttacg
     1441 cgaaaggcgg taaggcgttg gcactttggc actcagggtt tgtccgagtg gtgaattgct
     1501 cacctgagag ttaatgtgaa ttgagcacaa gtctagccga agaccatgac ccgtatccta
     1561 attgccgacg accatgaggt cgtacgttcg gggctccgga aaatcctcga atcccaaccc
     1621 aactgggaag tcgtcgccga ggttggcgat ggcaaggacg ccatcagcaa ggccacggaa
     1681 accagacccg atgttgccgt actcgattac tcgctgccgc tgatcaacgg catcgaggca
     1741 acacgccaga tccgggcacg cctaccgaag accgaagtcc tgatcttcac gatgcacgac
     1801 aacgagacgc tgatctcgga gctgctgaaa gcgggcgccc gcggctacct cctgaaatcc
     1861 gatgcgcaga gccacctgat cggcgcaatt gaatgtttgg ccctgcacaa gcccttcttc
     1921 acggccaagg tctcggaagc ccttttggac tccttcctga agcggcccgc ccgggaagga
     1981 tccatgctca ccaaccggga gcgctgcgtg gtgcagctga ttgccgaagg tcataccaac
     2041 aaacaagtgg cgaccattct cagcattagc ctgaagacgg tggagacgca tcgctcggcc
     2101 atcatgcgca agctcaacct ctcctcgtca gccgggctcg tccgttacgc gatccgcaat
     2161 aatatcgtgc aggcctgacg ccccccgcgt ctcgctctct gagctcagag ccgcggcgct
     2221 cggccatccc tgcacacagc gcgaggcgcc gcctcgtctt accgagcggc gccgggcgct
     2281 ctcggcttca acccttgagc cgtttgttgc attcgctgta gtagccgccg cccttcatga
     2341 tccagcgcat cccgccattg ccgttggtcg ccttgttggc gttgtactga tcaaggcagg
     2401 tgtgcatacg cgcgcgccct gccgattcct tcgtgtactt gggcgagacg gctttgggga
     2461 aggttgc
//
LOCUS       DASTMT010000365        13258 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_197540, whole genome shotgun sequence.
ACCESSION   DASTMT010000365 DASTMT010000000
VERSION     DASTMT010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 13258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13258
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_197540"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..178)
                     /locus_tag="VFR00_08495"
     CDS             complement(<1..178)
                     /locus_tag="VFR00_08495"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEU0159339.1"
                     /translation="MLKKTSSVLIGSMGMAQRSDRQRAVKDLKRALILDAARKVFERE
                     GLEGASLRAIATAAG"
     gene            233..1045
                     /locus_tag="VFR00_08500"
     CDS             233..1045
                     /locus_tag="VFR00_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009469400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HEU0159340.1"
                     /translation="MSVRIEKTGPVWTVIHDRPEARNAMDPESADALTAAFLEFDADR
                     EAAVAVFYGAGGAFCAGWDLKYVSTLSAEYPLGELDIPVAGAAGNGGEIPRGPLGPSR
                     LELDKPVIAAIEGPAVAGGMELGLWCDFRVMAEEAYFGVYCRRWGVPLIDGGTVRLPR
                     IVGQGRALEIILTGRKVPAAECLSLGLCEYVTPTGGARAKAEELAHEIARFPQVCVRA
                     DRRAAIKGQGLSVREALIQEWYNGREALKVDGVAGAGRFKSGLGRHGDFARI"
     gene            1230..1937
                     /locus_tag="VFR00_08505"
     CDS             1230..1937
                     /locus_tag="VFR00_08505"
                     /inference="COORDINATES: protein motif:HMM:NF036686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0159341.1"
                     /translation="MPRHFGILIPSTNTTCEIEFCRLPPDLQVHTARLGKAKGSPFTP
                     SLEADVAYQAQLLADARVEVIALAQTSASLFADDYDAVTIRRMTEASGVPSLTSAEAV
                     GRALRALGTRKVAMATPYSDEVIERAKRYYAQKFAIDVVASESLGATNSYAIGKMDAG
                     AAQAAFRRIDRADVEALVVPGGNFPTMHRIADWEREFGKPVVTTNQATLWAVLGAMRI
                     TTPLVGKGRLLETLPQD"
     gene            2023..3210
                     /locus_tag="VFR00_08510"
     CDS             2023..3210
                     /locus_tag="VFR00_08510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018066681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEU0159342.1"
                     /translation="MITDAARLQDLLANTRRFVTEVAIPNEERVEREDRVDAAILAEL
                     RRLGTFGWSIPTAYGGSELTQEELALAFVELSQCSVAYRVVGAQNAGIGSECLIRDGT
                     AEQKQIYLPRLATGDLIGCLALTEEGAGSDATALATRAERLSDGSYVLNGSKRYITLA
                     PIAGLFTVLARTDTRARGAAGISAFLIERGTPGLTTSAPTPKMGQEGAPIGEVYLRDC
                     RVSGDAIIGLAPGQGFATVMKCLNKQRINLAALSTGPAIRLLNEGIAYARERRQGGRA
                     IAEFQLVQAMLAECKVDIEAARALILETARKRDRGEDVTMDVSLCKYLATEMCCRVAD
                     RIVQVFAGQGYVKARGIERFYRDVRAARIYEGTSQIHLLNIAKHLLRQAPGAEPPIAD
                     AIS"
     gene            complement(3320..3718)
                     /locus_tag="VFR00_08515"
     CDS             complement(3320..3718)
                     /locus_tag="VFR00_08515"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU0159343.1"
                     /translation="MPIQKMDHFTILTKDWRATAEFYEDLLGFKAGARPNFAFPGCWL
                     HNDGKPILHVIERPEIPKETGLLDHMAFSASGLADYIGKLKARNVKYDLRRVPEGGYA
                     AGVWQVFFLDPNGAKVELDFAADEVAPAGA"
     gene            4489..6726
                     /locus_tag="VFR00_08520"
     CDS             4489..6726
                     /locus_tag="VFR00_08520"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEU0159344.1"
                     /translation="MQRATIYERPRVEAKVGETVVTSTCGHNCGGRCVVNAHVVNGRI
                     VKISTDPRRWECELPPLHACARGVGQIERVYHPDRLQYPLRRKGPRGSGAFERITWDE
                     ALQEVARQLLRVRAHYGNAAILDASRSGSLSVLHGRAAAQRFLYMFGGCTVLWSNMSA
                     EAEVFAVRMTYGTEANYKSAGREPTDYANSKLIIMWGWSPGDGTFGTGTYQYLKHAKK
                     QGVRIVCVDPRRTRSSLVLADEHVFIQPSTDTAALIAMAYVIASEGLHDRTYCDRHVL
                     GFDEAHLPPGAPPGSSYWSYLFGLTDGIVKTPEWAAAITGIPAETIRRLAIEFATTKP
                     AALQCGYAPGRTAFGEQFHRAAYALCAITGNVGIPGGSSGVSNGATGKSGIKNLPTGA
                     NPLKSRVASPLLADLLARGKAGGYPADIKLIYSVGGDLFNQAPNVNKIVGALDSIEVL
                     VAQDHFLTPTARYADIVLPATTFWERNDVHTPWAGAGHYAIYMRQAIEPMYECRNDID
                     IFADLAVRVGITGYNDKSEVAWLRELTKGSVDDFGAFVDNGVARFAPPEDATAFAAEI
                     RDPERHRFTTPSGKIEIYSMALAAKPDPYGLGAIPPIPTWIPQLESDVRYPLKLCTPK
                     SRGRTHSIHGNQPMLARVDRDDIWLNPADARTRGITDGERVRVFNDRGSTVLPARITT
                     RMARGAVSIKEGAWFTPDASGTDSKGCANVLTDDRSAPCGATTYNSNLVEVERALPER
                     EVTSA"
     gene            complement(6917..7555)
                     /locus_tag="VFR00_08525"
     CDS             complement(6917..7555)
                     /locus_tag="VFR00_08525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0159345.1"
                     /translation="MIDKLPASVILSEGSKTSAGSWVVPVMVLERLKLTAPARLPQGL
                     EFSIALVADGAVLVERTVALYVAPAAVTARPDNKVDETQAPGIAAFTTSSVGSAPRDP
                     PDRNGRPAGPSNEERAEAERLVSRGERHLADGNITIARQYFLRASELGLATAALKMAE
                     THDARALAGINVRGLVPDPAEAKKWYERALELGATEAQVRLQRLGSMNPGKP"
     gene            complement(8185..9132)
                     /locus_tag="VFR00_08530"
     CDS             complement(8185..9132)
                     /locus_tag="VFR00_08530"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEU0159346.1"
                     /translation="MDLQQLRTFIAIADSGGVARAAQRLHLSQPAASRQLQALEADLG
                     MLLFDRIGRRMRLTSEGEDLVPRARKLLAEAQSLRQRARALQSGETGQVRIGATPPMI
                     ESVLAGFLGKYRRRHPGIDISVVEDGGAGLIARLARGEVQLAYVPVGDPRFSGRLLYP
                     IHVMAVLPEAHPLGRGRCLDIAKLAGEPLLLLNRSFASRQWFDAACQRASMAPNVRLE
                     SGAPNVVLGLAEANYGIAILPSMVRLSRKGLRALALVDRQAAIGQWTRLAWDPQRFLA
                     PYVAAFGEALVDHAQRAYPGRELIRRAPALIRPVEAKPR"
     gene            9239..9526
                     /locus_tag="VFR00_08535"
     CDS             9239..9526
                     /locus_tag="VFR00_08535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016840533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0159347.1"
                     /translation="MSRRDSHDHRLDSLVLDLIEWVSVSPRPYAEVMEVWRTTCARLP
                     IWEEAVDRGFLVSEMAAPAGVLVQVTPAGRAFLELCRGRDRQKDGHAAQLR"
     gene            9623..10225
                     /locus_tag="VFR00_08540"
     CDS             9623..10225
                     /locus_tag="VFR00_08540"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HEU0159348.1"
                     /translation="MQLYVTLTSPYARLARILVLEKGLGDRVAIIEAKTRTVGSPYYA
                     INPSGRVPYLIDASGVGLEDSQLICAYLDQLDGAPRFHLPADSSNWAYRRLEATARSM
                     CDGIAVWVREMARPANERSPTVLAHEVARSDRLADVFEARVSDAELKGAPKMAHFILA
                     AALDMAHKRGSGDLTRGRPGLAAWHRDISELPSLRATALP"
     gene            10418..11533
                     /locus_tag="VFR00_08545"
     CDS             10418..11533
                     /locus_tag="VFR00_08545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012329652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0159349.1"
                     /translation="MYRVMLAAAAAVLVLSPVELAAQNGGPPQYLVEPFWPKPLPDNW
                     ILGQVAGVAVDNDDSVWIIHRPSTLVDDEKGAEKTPPETRCCKAAPPVLHFAADGKLL
                     ASWGGPGAGYEWPATEHGIHIDKAGNVWLAGNGKDDHQILKFTPDGKFLQQIGKAGST
                     SGSNSKTALGRPAHMVTDDAAGELYVADGYLNRRIVVFDAKTGEYKRHWGAYGSKELS
                     DDKLPAYTPVAISALSKSFANPVHCVRLARDGMVYVCDRANDRIQVFQKDGTFVREFQ
                     VEPQTLQNGSVWDLVLSEDAAQRYIFVADGANMQINTLDRQTGEKLAQFGRPGRMAGE
                     FKWVHNMAIDSKGNLYTTEVGTGRRVQKFKRSGGPTN"
     gene            11921..12910
                     /locus_tag="VFR00_08550"
     CDS             11921..12910
                     /locus_tag="VFR00_08550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0159350.1"
                     /translation="MSVRLGSGAASAPAIFVMHENSDWVKPLRREFAALGAPYQEWFL
                     DSGSLDLGAPPPEGVFYNRMSASSHTRGHRYAPELTGAVLAWLTRHGRRIVNGERALV
                     LELSKVAQYQALAKFDIEVPATIAAVGSMAIAAAATELGYPLILKHNRAGKGLGVKLI
                     YSAEALQQHLASPDFVHSVDGLMLVQRYIAAPAPFITRLEFVGGRFLYAVRVDASAGF
                     ELCPADACQVEPAGVCPAVAPSDKFQILPRFEHPLLPVWQAFLAANDIGIAGIEFIGD
                     AEGRAYTYDVNTNTNYNPDAELKDGRRGMAAIARHLHELMLATYPRAAIASAA"
     gene            13128..>13258
                     /locus_tag="VFR00_08555"
     CDS             13128..>13258
                     /locus_tag="VFR00_08555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017996489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU0159351.1"
                     /translation="MATVDIRNVKKAFGSVEVLHGVSVEIADSEFVVLVGPSGCGKST
                     "
BASE COUNT         2347 a         4296 c         4324 g         2291 t
ORIGIN      
        1 agcccgcggc cgtggcgatg gccctgagcg aggcgccttc gagtccctcg cgctcgaaca
       61 ccttgcgcgc ggcatccagg atcaatgccc gcttcaggtc tttgaccgcc cgttgccgat
      121 cactgcgctg cgccataccc atgctgccta ttagcacact tgacgttttt ttcaacattg
      181 ttagattttt tgttacgctc ggcaaaatcg cagcaatgag aggtgcgagg cgatgtcagt
      241 cagaattgaa aaaaccggac cggtctggac ggtcatccat gaccgccccg aagcccgcaa
      301 cgccatggat ccggagagcg ccgacgcctt gacggcggcc tttctcgaat tcgacgccga
      361 ccgggaggcg gcggtggccg tgttctatgg tgcgggcggc gccttctgcg ccggctggga
      421 cctcaagtat gtgagcacgc tgagcgccga gtatcccctg ggcgagctcg acattcccgt
      481 ggccggcgcg gctgggaatg gtggtgaaat cccgcgcgga ccgctgggac cttcccggct
      541 cgaactcgac aagccggtga ttgcggcgat cgaaggcccc gccgttgccg gcggcatgga
      601 gttgggcctg tggtgcgatt ttcgggtcat ggccgaagag gcctatttcg gcgtctactg
      661 tcggcgctgg ggggtgccct tgatcgatgg cggaacggtg cgcctgccgc gcatcgttgg
      721 gcaaggccgt gccttggaga tcatcctgac gggccgcaag gtgcccgcgg cggaatgctt
      781 aagcctcggc ctttgcgaat acgtgacccc cacaggcggg gcccgcgcca aggctgaaga
      841 gctcgcgcac gagatcgccc gcttcccgca ggtgtgcgtc cgcgccgatc ggcgtgcggc
      901 cattaagggc caggggctca gcgtgcgcga ggcgctgatc caggagtggt acaatggacg
      961 cgaagccttg aaggtcgatg gcgtggcggg cgccggccgc ttcaaaagtg gtcttggacg
     1021 ccacggcgat tttgcgcgca tttagctgca agaggcatct tgtttgcccg cgctggcggc
     1081 gattttcggg gcgcgccgcg gcgctgcaac gcgaccgtgg ctagcgagca cgcgccggcg
     1141 ccaattgcgt tgcggcgccg aacctgctgg tattcgcctc tgccctcgcc cgtgggcagg
     1201 attgctcaca cccaacgggg gacatcccta tgccgcgtca tttcggcatc ctcattccct
     1261 ctaccaacac cacctgcgag atcgaatttt gccgcttgcc gcccgacctg caggtgcata
     1321 cggccaggct tggcaaagcc aagggctcgc cttttacccc cagcctggag gccgacgtcg
     1381 cctatcaggc gcaactcttg gcggacgccc gcgtcgaggt catcgcgctg gcgcagacct
     1441 cggccagttt gtttgccgat gactacgacg cggtcaccat ccgccgcatg acggaggcct
     1501 cgggcgtgcc gtccttgaca tcggccgagg ccgtcggtcg ggcgctgcgc gcgctcggca
     1561 cgcgcaaagt cgcgatggca acgccttact ccgacgaggt catcgagcga gccaaacgct
     1621 actacgccca gaaattcgcg atcgacgtcg ttgcctccga aagtctcggg gccaccaatt
     1681 cctatgccat cggcaaaatg gatgccggtg ccgcgcaagc cgcgttcagg cggatcgatc
     1741 gcgccgacgt cgaggccttg gtggtgccgg gtggcaactt tccgaccatg caccggatcg
     1801 ccgactggga acgcgagttc ggcaagccgg tggtgacgac caatcaggca acgctttggg
     1861 cggtgctggg ggccatgcgc atcaccacgc cgctcgtcgg caaggggcgc ttgctcgaga
     1921 cgctgccgca ggactaggcg ccgcgcgcaa aagccgaatg gcagtcgcgc gcacgcgggc
     1981 ttgtctgctt cagcgcgcca gggtcgataa aggcggaacg ccatgatcac cgatgccgcc
     2041 cgcttgcagg atctgttggc caatacccgt cgcttcgtca ccgaggtcgc catccccaac
     2101 gaggagcggg tcgaacgcga ggaccgggtc gatgccgcca tcctcgccga actgcgccgg
     2161 cttggcacct tcggctggag catccccacg gcctacggcg gtagcgaact gacccaggag
     2221 gagctggccc tcgccttcgt cgagctcagc caatgctcgg tcgcctaccg cgtcgtcggg
     2281 gcgcagaatg ccgggatcgg ctcggagtgc ctgatccgcg acggcacggc agagcaaaag
     2341 cagatatatc tgccgcggct tgccaccggt gatctcatcg gctgcctggc gctgaccgaa
     2401 gagggcgcgg gctcggatgc cactgcgctg gcgacgcgcg ccgaacgctt gtcggacggc
     2461 agctacgtcc tcaacggttc gaagcgctat atcacgctgg ccccgatcgc cggcctcttt
     2521 acggtgctgg cgcggaccga tacgcgggca cgcggcgcgg cgggcatctc cgcattcctc
     2581 atcgagcgtg gcacgcctgg gctcacgacc agcgcgccga cgccaaaaat ggggcaggag
     2641 ggcgcgccga tcggcgaggt ctatttgcgg gactgccggg tctcgggcga cgcgatcatc
     2701 ggcctcgcgc ccgggcaggg ttttgcgacc gtaatgaagt gcctgaacaa gcagcgcatc
     2761 aatctggccg ccttgtcgac ggggccggcc attcgtctcc tcaacgaggg catcgcttat
     2821 gccagggaac ggcggcaagg aggccgcgcc attgccgagt ttcagctcgt gcaggccatg
     2881 ctggccgaat gcaaggtcga catcgaggcc gcccgcgccc tgatcttgga gacggcgcgc
     2941 aagcgcgacc gcggcgagga cgtaaccatg gacgtgtcgc tctgcaagta tctggcaacg
     3001 gagatgtgct gccgcgtcgc cgaccgcatc gtgcaagtct ttgctggcca aggctacgtc
     3061 aaggcgcgcg gcatcgagcg cttctaccgg gatgtgcgtg ccgcccgcat ctacgagggc
     3121 acgagccaaa tccacctcct caatattgcc aagcatctgt tgcgccaagc tccaggggcc
     3181 gagccaccga tcgccgacgc catatcgtga ggaaagcgaa tgaggccgag gtgctcgatc
     3241 cctggaaggg cccggagcat ctggccttgc aacgggaacg ggtgctctcg ttcttggaga
     3301 cgcacacgcc ggtcggcgac taggcgccgg ccggtgcaac ctcgtcggcg gcgaaatcga
     3361 gttcgacttt ggcgccgttg gggtcgagaa agaacacctg ccagacgccg gcggcatagc
     3421 cgccctcggg cacccggcgc aggtcgtact tgacgttgcg cgccttgagc ttgccgatgt
     3481 aatcggccag gccgcttgcg gaaaaggcca tgtgatcaag cagccccgtc tccttgggaa
     3541 tttcgggacg ctcgatgacg tgcaggatgg gcttgccgtc gttgtgcaac cagcagccgg
     3601 gaaaggcgaa gtttggccgc gcgccggcct taaagccgag caggtcctca tagaactccg
     3661 ccgtcgcccg ccagtccttg gtgaggatgg tgaaatggtc cattttttgg atcggcataa
     3721 cgttttgatc ctgctggtgt tgctggaatg agagttaagg gttgggaggt actttcgcct
     3781 gttgttgcct caggggtcat gtctaaaata ccccgggaaa atcgtccagg ctattgggac
     3841 gcccgcaagc gtctcaatcg cagcgacgcc cattccggat ctcggtgcaa agcgtgcgcc
     3901 gccatggtgt tgcgcaacct cctgaaatgc tgggcatcga cgacggcagc acggggccgg
     3961 cgatcctcgc agctgctgcg cttcacgata ctgggcgagg agaccgacac ttccgaatag
     4021 gagggacggc tgctattcgg cgatcagatc cgcgtctctt ggtcctggca ggacaggcgc
     4081 caaggcacga cccaaggcgg cgcgcaagcg ccagcaaatc gaagcatgcc cagcctatga
     4141 gccgagaggc cgcgctcgtg aagctcgcgg ataatctgcg caacgcggca gcaaattcag
     4201 cgagggattg cagcatcgag cggcgcaccg aatacgtcga atggacaaaa gcggtgatgc
     4261 taaattgccg cggtgggccg cgagctcatt gggttgtgca aaatggccgc gtgaccgacg
     4321 tcgaccgcgc ttggatccgg gagcaatgtc cggctcgggc tccggcgaga gccaggggcc
     4381 gggcctagcg tcctccttgg tttcgcgacg cgggcaggcc atactggctc gcacctatcg
     4441 tttgcggcgc gcgtggccaa ggccgaatca gcaaccgaga ctcagggaat gcaacgggcg
     4501 acgatctacg agcgaccgcg ggtcgaggcc aaggttggcg agaccgtggt taccagcacg
     4561 tgcggtcaca actgcggggg ccgctgcgtg gtgaatgcgc acgtcgtcaa cgggcgcatc
     4621 gtcaagatct ccaccgaccc ccgccggtgg gagtgcgaac tgccgccgct gcacgcctgc
     4681 gcccgcggcg tgggccaaat agaacgcgtt tatcaccctg accgcctgca gtaccccctg
     4741 cgccgcaagg ggccgcgcgg cagcggcgcc ttcgagcgca tcacctggga cgaggcgttg
     4801 caggaggtcg cccgccagct cttgcgcgtg cgcgcgcact atggcaacgc cgcaatcctc
     4861 gatgcgtcgc gttccggcag cctttcggtt ctgcacgggc gggcggcggc gcagcggttc
     4921 ctttacatgt tcggcggttg cacggtgttg tggtcgaaca tgtcggcgga agccgaggtg
     4981 tttgccgtgc gcatgactta cggtaccgag gccaattaca agagcgctgg gcgcgagccc
     5041 accgactacg ccaactcaaa gctgatcatc atgtggggct ggagccctgg cgacggcacc
     5101 ttcgggacgg gcacctacca atatctcaag cacgccaaga agcagggcgt gcgtatcgta
     5161 tgcgtggacc cgcgcaggac ccgttcgagc ctcgtactcg ctgacgagca cgttttcatc
     5221 cagccctcga ccgacacggc cgcgctcatc gccatggcct acgtgatcgc cagcgagggc
     5281 ctccacgatc ggacctactg cgacagacat gtgctgggct tcgacgaggc gcacttgccg
     5341 cccggtgcgc caccgggctc atcctattgg tcttatctct tcggtctcac cgatggcatc
     5401 gtcaagacgc ccgaatgggc tgccgcgatc accggcatcc cggcagagac cattcgccgg
     5461 ctcgccatcg aattcgccac caccaaaccc gctgccctgc agtgcggcta cgcaccgggt
     5521 cgcactgcct tcggtgaaca attccatcgt gccgcttacg cgctctgcgc gatcacaggt
     5581 aacgttggca ttcccggcgg cagttcggga gtaagcaacg gtgccactgg gaaaagcggc
     5641 atcaagaacc tgccgacggg tgccaacccg ctgaaatccc gcgtggcttc gccgctgctc
     5701 gccgacctac tggcgcgcgg caaggccggc ggctatccgg cggacatcaa gctgatctat
     5761 tcggtcggcg gcgatctttt caatcaggct cccaatgtca acaagatcgt cggcgccctc
     5821 gacagcatcg aggtcttggt ggcgcaagat cacttcctga cacccacggc ccgctatgcg
     5881 gatatcgtgc tacctgccac taccttctgg gagcgcaatg acgtgcacac gccatgggcc
     5941 ggcgcggggc attatgcgat ctacatgcgc caggcgatcg aaccgatgta tgagtgtcgc
     6001 aacgacatcg atatctttgc cgaccttgcg gtgcgcgtcg gtattaccgg ctacaacgac
     6061 aagagcgagg tcgcttggct gcgcgagctg accaagggtt ccgtcgacga cttcggtgcc
     6121 tttgtcgaca atggcgttgc ccgcttcgcg cctccagaag atgcgacggc gttcgcggcc
     6181 gagattcgcg accccgagcg tcacaggttc acgacgccct cgggcaagat cgagatttac
     6241 tcgatggcgc ttgcggccaa gcccgacccc tacgggctcg gcgcgatccc gccgatcccg
     6301 acctggatac cgcagttaga atcggatgtg cgttacccac tgaagctctg cacgcccaag
     6361 tcgcgcggtc gcacgcactc cattcacggc aatcagccga tgctggcgcg cgtcgaccgc
     6421 gacgatatct ggctcaatcc tgcggacgcg cgaacgcgcg gcatcacgga tggggagcgc
     6481 gtgcgcgtgt tcaacgaccg gggctccact gtgctgccgg cgcgcatcac cacgcggatg
     6541 gcgcgcggcg ccgtatcgat caaggaaggc gcctggttca cgcccgatgc ctcgggcacg
     6601 gacagcaaag gctgcgccaa tgtgcttacc gacgaccgct cggcgccctg cggggcaacg
     6661 acgtacaatt ccaatttggt ggaggtggag cgggctttgc ctgagcgcga agtaacaagc
     6721 gcataatgcg ccgcgtggat cgcgagagag tgcaatcaaa cgatagacat cgagagggaa
     6781 actgatccgc ccaacgtcgg gccgaccgat acgccctcga tgacatcatc gtcgctgccg
     6841 aggaaacagc ggtggacgat tgtgggacgc ctcgccgatc aacgcttttc ctgacgcaaa
     6901 gacgcggttg agcctatcaa ggtttgccgg ggttcatact ccccaaccgc tgcaatcgaa
     6961 cttgtgcctc tgtcgcgccg agctcaagcg cccgctcata ccactttttc gcttcggctg
     7021 ggtcgggcac taggccgcgc acgttgatgc ccgcaagagc ccgcgcatcg tgcgtttctg
     7081 ccattttcag tgctgcagtt gcgagcccaa gctcggaggc acggaggaaa tattggcgcg
     7141 cgatggtgat gttgccgtcg gcgagatggc gctcaccccg gctcacgagc ctctccgctt
     7201 cggcgcgctc ctcgttgctg gggccggccg gccggccgtt ccggtccggg gggtccctgg
     7261 gcgccgagcc aacggacgag gtggtaaagg ccgcaatgcc tggtgcttgc gtctcgtcca
     7321 ctttgttgtc agggcgcgcg gtgacggcag cgggggcgac gtaaagtgca actgtccgct
     7381 cgacgagcac cgcaccatcg gcaaccaaag cgattgagaa ctctaaccct tggggtaggc
     7441 gagcgggggc ggtgagttta agtctttcga gcaccatcac cggcaccacc caagatcccg
     7501 ctgaggtctt gctaccttct gacaaaatga cagaggcagg cagcttatcg atcacaacgt
     7561 agctgcgcgg cggcaaagcg tcagtggaac cgcccacacg cacatagaat tccgtgggtg
     7621 ttgcggcctc tacctctaga gttggtgcga ccgcaatttg aggccgtgcc ggacccgtcc
     7681 tatttccttc cccagggtcg gccgcgccgg cgtctacggt cattgggctg agcaaggtgg
     7741 cgagcaaact tgcaagcgcc acccagaacc gaattcgaca ctgacgcggg ccgcttaggc
     7801 ttccgccaat tgccgcgacg agcacatttg acacacggcc tcgccctgtc atggcaaacc
     7861 cctccacgca cacagctagc atctcaaggg tgcttttttg gggcgccgcg tcaacgcaac
     7921 gacgaggctt accgccccgg cccatgcccg cacatcgtcc cgaagatccc gcaccttcaa
     7981 tgacctgcgg gagcaactta atacgctgtg gcctgaacgt cagtaggaaa gatgacgtta
     8041 accctgcctg agcatcggtc cactgaaaat tcagataacc gcgccttcag agtaaagata
     8101 aaccctggat attgctgggc agggcaccca ttcgtacgta accttgagtg tgggcgtgtt
     8161 cgcgctttcg caggcgcccg aggcttatcg gggcttggcc tcgaccgggc ggatgagcgc
     8221 cggggcgcgg cggatcagct cgcggcccgg ataggcgcgt tgcgcatggt caactaaggc
     8281 ctcgccgaag gctgccacat agggggccaa gaagcgctgc ggatcccagg ccaggcgggt
     8341 ccactgaccg atcgcggctt gccggtcgac aagggcaagc gcgcgcaggc ctttgcgaga
     8401 cagccgcacc atcgagggca ggatggcaat gccatagttg gcttcggcca ggccgaggac
     8461 gacattgggg gcgccgctct cgagccggac gttgggagcc atgctggccc tttggcaggc
     8521 agcatcgaac cactgccgcg aggcaaagct gcggttgagc agcaagagcg gctcgccggc
     8581 cagcttggca atgtcgaggc agcgtccgcg accgaggggg tgcgcctcgg gcaggaccgc
     8641 catgacgtgg atcggataga gcaagcgccc gctgaagcgc ggatcgccga cgggaacgta
     8701 ggccagctgc acctcgccac gggccagccg ggcgatcagt ccagcgcccc cgtcttccac
     8761 cacgctgata tcgatgccgg gatggcggcg gcgatatttg ccaagaaagc cggcgagcac
     8821 cgactcgatc atgggtggcg tggcgccgat gcgcacctgc cccgtctcgc cggactgcaa
     8881 ggcccgcgcg cgctggcgca gcgactgggc ctcggccaac agcttgcgcg ctcgcggcac
     8941 gaggtcctcg ccctcggacg tcaggcgcat gcgacggcca atgcgatcga acagcagcat
     9001 ccccaggtcc gcctcgaggg cttgcagctg ccgcgacgct gccggctggc tcaaatgcaa
     9061 gcgctgggcg gcgcgcgcga cgccgccgct gtcggcgatg gcgatgaagg tgcgcagctg
     9121 ctgcaggtcc acgcctttat gatcgttttg catcataatt gatatgataa atgcattgga
     9181 cagattaagc cagcgtgcgg catgctgcgt aatcctttgc ttcgccgagg tgggccgcgt
     9241 gagccgtcgg gacagtcatg accatcgcct cgattccctc gtactcgacc tgatcgagtg
     9301 ggtttcggtt tccccgcgcc cctacgcgga ggtgatggag gtgtggcgca ccacctgtgc
     9361 ccgcctgccg atctgggagg aggccgtcga ccgcggcttt ctcgtcagcg aaatggccgc
     9421 gcctgcaggc gtgctggtgc aggtgacgcc ggcgggacgc gcgtttcttg agctctgccg
     9481 gggtagagat cggcagaaag acggccatgc cgcgcaactg cgctaggcga gccgcgccga
     9541 tgccgatgcg cgcgcggcac ttgtcgtgct aagcatgcgc gccagtgcct cctcctctcc
     9601 tcctccaaca gcgatgcccg ccatgcagct ctacgtcaca ctcacctcgc cttacgcgcg
     9661 cctggcgcgc attctcgtgc tcgagaaagg cctcggcgat cgtgtcgcca ttatcgaggc
     9721 gaagacgcgc acggtaggca gtccctacta tgccatcaat ccctcggggc gcgtgccata
     9781 cctgatcgac gccagcggcg tcggcctgga agacagtcag ctgatctgcg cttatctcga
     9841 tcagctcgat ggcgcgccgc gatttcattt gcccgcggat agctcgaact gggcctaccg
     9901 caggttggag gcaaccgcgc gcagtatgtg cgatggcatc gccgtgtggg tgcgggaaat
     9961 ggcccggccg gcaaacgagc gctcgcccac cgtgcttgcc cacgaggtcg cccgcagcga
    10021 ccgcttggcg gatgtcttcg aagcgagggt cagcgacgcc gagctcaagg gcgccccgaa
    10081 gatggcgcac tttatcttgg cagcggcgct cgacatggcg cacaagcgcg gctcgggcga
    10141 cctgacgcgg ggacgcccag ggcttgccgc ttggcatcgg gacatctcag aactgccctc
    10201 gctgcgcgcc accgccttgc cttgaggtcg cgcctcgccg gcgcaggctc gttggcgcgc
    10261 acgcctcttc ttcggggctc tcgcgccatc tcgaggctcg cggcttaagc attcgacgca
    10321 gtcgtggctt ggcgctactc tgggccactt ttcccgcgcc tggcgcggca aagggcgcct
    10381 cggcgcgcca acggacgaca gcttggagga accccccatg taccgcgtga tgctggcggc
    10441 cgcggccgcc gtccttgtcc tcagccctgt cgagcttgcc gcgcaaaatg gcgggccgcc
    10501 gcaatatctg gtcgagccgt tctggcccaa gccgttgccc gacaattgga tcttgggcca
    10561 ggtcgcgggc gttgccgtcg acaacgacga cagcgtctgg atcatccacc gcccctcgac
    10621 gttggtcgat gacgaaaagg gcgccgaaaa gacgccgccg gaaacgcgct gctgcaaggc
    10681 ggcacccccc gtgctgcact tcgccgccga cggcaagctg ctggccagct ggggtggacc
    10741 tggggcgggc tacgaatggc cggccaccga gcacggcatt cacatcgaca aggccggtaa
    10801 tgtctggctc gccggcaacg gcaaggacga ccaccaaatc ctgaagttca cgcccgacgg
    10861 caagttcctg cagcaaatcg gcaaggccgg cagcaccagc ggctccaaca gcaaaacggc
    10921 gctgggtcgc ccggcgcaca tggtgacgga cgacgctgcc ggcgaactct atgttgccga
    10981 cggctatctc aaccggcgta tcgtcgtgtt cgatgcaaaa accggcgagt acaaacgcca
    11041 ctggggggcc tacggcagca aggagctgag cgacgacaag ctgccggcct acacgccggt
    11101 ggcgatcagt gcgctgtcga agtcgtttgc gaaccccgtg cactgcgtgc gcctggcgcg
    11161 ggacggcatg gtctacgtct gcgatcgggc caacgaccgc atccaggtgt tccaaaagga
    11221 cggcaccttc gtcagggagt tccaggtcga gccgcagacg ctgcaaaacg gttcggtctg
    11281 ggatctggtg ctgtcggagg atgcggccca gcgctacatt ttcgtggccg atggcgccaa
    11341 catgcagatc aacacgctcg accggcagac gggcgagaag ctggcgcaat tcggtcgccc
    11401 cggccgcatg gccggcgaat tcaaatgggt gcacaacatg gcgatcgatt cgaaaggcaa
    11461 cctctacaca accgaggtgg gcaccggacg ccgcgtgcag aaattcaagc gcagcggcgg
    11521 cccaaccaac tgatagggcc gcgcgccggc ggtcgtcccg catggcgcgc cgggctgctc
    11581 gcgacaccga gaagatctgt cgcgccgggg cgcgacggtc ctctcacctg ggtggggcgg
    11641 gcgtgctcgc tctcttgccc gcgtggcacg gcccgggctc gggcggcggt agggcgcttg
    11701 cctcgccgcg cctcggcctg cccctgggct ggctggcaat actcaattct gcgcgtgcgc
    11761 tggaatgagc cgtgcgtgcc aagccgcgtt tagagaacga gatttccctc gaaaaaaccg
    11821 gcccttcaaa ggccctgcca agtgcgccat atgacggcga cggcgcgttg tcgcgcacga
    11881 gctgcgtcga tgcgccgact tgagcaaatg aggatcgagg atgtctgtgc ggcttggcag
    11941 cggcgcggcg agcgcgccgg cgatttttgt gatgcatgag aactcggatt gggtgaaacc
    12001 gctgcggcgc gagtttgccg ccctcggcgc gccttatcag gaatggtttc tcgatagcgg
    12061 cagtctcgac ctcggcgcgc caccgcccga gggagttttt tataaccgca tgagcgcatc
    12121 atcgcacacg cgcggacatc gctacgcgcc cgaattgacc ggcgccgtct tggcgtggtt
    12181 gacgcggcat ggacgccgga tcgtcaacgg cgagcgcgcg cttgtgctcg agctctccaa
    12241 ggtggcgcaa tatcaggcgc tggcaaaatt cgacatcgag gtgcccgcca ccattgccgc
    12301 cgtcggcagc atggccatcg ccgcagccgc caccgagctc ggatatcctc tcatcctcaa
    12361 gcacaaccgg gccggcaagg ggctcggggt caagctcatc tattccgccg aggcgctgca
    12421 gcaacacttg gcaagccccg acttcgtcca ttccgtcgac ggcctcatgc tggttcagcg
    12481 ctacattgcg gcgccggccc cgttcattac gcgcctggag ttcgtcggcg gccgctttct
    12541 ctacgcggtg cgcgtcgacg cctccgcagg ctttgagctt tgcccggccg atgcctgcca
    12601 ggtcgagccc gccggcgttt gcccggccgt cgccccaagc gacaagttcc agatcctgcc
    12661 gcgcttcgag catccgctgc tgccggtctg gcaagccttt ctggccgcca acgacatcgg
    12721 catcgccggc atcgaattca ttggcgatgc cgaggggcgc gcctatacct acgatgtcaa
    12781 caccaacacc aactacaatc ccgatgccga attgaaagat gggcggcgcg gcatggccgc
    12841 cattgcccgt cacttgcacg agctgatgct tgcgacctat cccagggccg ctatcgccag
    12901 cgcggcgtga gcgcgaggcg cggccgctgc ggccccctct gagcaaccgc agcatggcgc
    12961 gacggcgatc gcgcctaatc gcgcaaagat gcgggaaagc agtccgcttg gagatcgacc
    13021 ctggttcgtt catagtgcgc ggaccggcag gccgaggtgc gcgccttaag ggttgcccgg
    13081 tgtaacaggt gcaaagccga tgcggctgca agacaccggg gggaggaatg gcaaccgtcg
    13141 acatccgcaa cgtgaagaag gcgttcggtt cggtcgaggt tctgcacggc gtcagcgtcg
    13201 aaatcgccga cagtgagttc gtggtgctgg tcgggccctc cggctgcggc aagtccac
//
LOCUS       DASTMT010000366         3628 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_4368326, whole genome shotgun sequence.
ACCESSION   DASTMT010000366 DASTMT010000000
VERSION     DASTMT010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3628)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3628)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3628
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4368326"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..233)
                     /locus_tag="VFR00_08560"
     CDS             complement(<1..233)
                     /locus_tag="VFR00_08560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007607851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEU0159352.1"
                     /translation="MSFTDIFIRRPVLASVVSLLILLIGLASAFNLPVRQFPKLTNTT
                     ITITTVYPGANADLIKGFITTPIQQAVASAEGI"
     gene            complement(246..1400)
                     /locus_tag="VFR00_08565"
     CDS             complement(246..1400)
                     /locus_tag="VFR00_08565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEU0159353.1"
                     /translation="MTRRLIITGLVVAVLLGALSAWQFAVKPMLVKSFLAQMVPPPAT
                     VTAEKATQEQWVEQLTSIGTLIASQGVDIASQVAGVVTEIPVESSMDVEQGAKLVQLD
                     TAVELADLASAKATLDEAEVAYARQVDLMQKKVTSEANLDTARAKRDTAAASVKRIEA
                     VIAQKAIVAPFAGRVGIRKVEKGQYVAPGMALISLQALDPIRVDFPMPEQTIGKLRVG
                     QTLQLTVDAYPGEVFQGEIQFLDARVAQETRTLLVRGLLRNRERKLLPGMFANVTVLA
                     GEPREVVTVPRTAVTFSLYGDSVYVAASAPAKPGEARAATPPEAPEPLIADRRFVRTG
                     EMREDRVAISSGVNAGELVVTTGQLKLNPGASIHIDNSQPLVRPEPRPKQ"
     gene            1759..2247
                     /locus_tag="VFR00_08570"
     CDS             1759..2247
                     /locus_tag="VFR00_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015666140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEU0159354.1"
                     /translation="MKRTLNPRREILFELSDVARALRTYVDQRAREHGMTRAQWGVLA
                     RLERDEGMVQAEMAEALEIQPISLVRLVDRLCEQGLVERRAHPTDRRANCLYLTEQGR
                     TRLAQMVPLGREISGNVLASFSESDVAEFLSKLLSLKRNIRRAAARRNALNGGSEVRH
                     AR"
     gene            2237..3334
                     /locus_tag="VFR00_08575"
     CDS             2237..3334
                     /locus_tag="VFR00_08575"
                     /inference="COORDINATES: protein motif:HMM:NF012739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HEU0159355.1"
                     /translation="MPASLFNRMRGLQRLPTLRAVLLLIIPALVGCLALAVYLFSGRY
                     VSTDNAYIGAQKVLITPEVSGKVVSIAVHEGQLLQPGDELLAVDPVPYRLAAQEAEAK
                     LARVKTDFENLKSSFASLGKQIELSRQALEAAQSDYDRKLALLNNRISTPSDVDRSRA
                     ALVAAKAQLEQLQQQEAGARIQLLGDANLPLERFPQYVEALASLDRARRDLTNTTLRA
                     PIAGVATQVASIQMGRYLVAGTAVFSIIDSANVWIDANPKETDLTYVRPGQPVDITVD
                     AFPSRHWTGVVGAISPGTGAQFSILPPQNAAGNWIKIVQRVPVRIEFAPGQDLRRLRS
                     GMSAMVEIDTGRVGRIASFFSSSAVAEGPEE"
     gene            3331..>3628
                     /locus_tag="VFR00_08580"
     CDS             3331..>3628
                     /locus_tag="VFR00_08580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007615966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0159356.1"
                     /translation="MIARGGSTFTPALQRTLITVCVMMATIMQALDITIANVALPYMQ
                     GSLSATLDQVSWVLTSYIVAAAIMTAPVGWLAGRFGIKRVLVTCAAGFTAASMLC"
BASE COUNT          629 a         1173 c         1181 g          645 t
ORIGIN      
        1 tcgataccct cggcactggc gacggcctgc tggatgggcg tcgtgatgaa gcccttgatc
       61 aggtccgcgt tcgcgcccgg gtagacggtc gtgatggtga tggtggtatt ggtgagttta
      121 ggaaactgcc gcacgggcaa attgaaggcc gatgccaggc cgatcagcag gatcaacagg
      181 ctgacaacgc tggcaagtac cgggcggcgg atgaaaatgt cggtaaatga catcggctcc
      241 agcccttact gcttggggcg cggctctggc cgcaccagag gctgcgagtt gtcgatgtga
      301 atgcttgcgc cgggattgag tttcagctgg ccagtggtca ccaccagctc gccggcattt
      361 acgccagagg agatggcgac cctgtcctca cgcatctcgc cggttctgac gaagcgccgg
      421 tccgcgatca gcggctcggg cgcctcgggc ggtgttgccg cgcgcgcctc gcctggtttg
      481 gcgggtgccg aggccgccac gtagacgcta tctccgtaga gcgagaaggt caccgccgtg
      541 cgcggcaccg tcacgacctc gcgcggctcg cctgcgagca cggtgacgtt ggcaaacatg
      601 ccgggcagca gcttgcgttc ccggttgcgc aaaagtccgc gcacgagcag cgtgcgcgtt
      661 tcctgggcga cgcgcgcgtc caaaaattgg atctcgccct gaaacacctc acccgggtag
      721 gcatcgacgg tgagctggag ggtctggccg acccgcaact taccgatggt ctgctcgggc
      781 atgggaaaat cgacgcgaat ggggtcaagc gcctgtagcg aaatcagcgc catgcccggt
      841 gcgacgtact ggcctttttc caccttgcgg atgcccacgc gtcccgcgaa cggggcgaca
      901 atggccttct gcgcgatgac ggcctctatg cgtttgaccg aggccgcggc ggtgtcgcgc
      961 ttggcgcgcg cggtgtcgag gttggcctcg gaggtgacct tcttctgcat aaggtcgacc
     1021 tgccgggcat aggcgacctc ggcctcgtcc agggtggctt tggcgctggc cagatcggca
     1081 agctcgacgg cggtgtcgag ctgcaccagc ttggcgccct gctcgacgtc catgctgctc
     1141 tcgaccggga tctcggtgac gacgccggcg acctgggaag caatgtcaac gccttgcgat
     1201 gcgatcaagg tgccaatgga cgtgagctgt tccacccatt gctcctgcgt cgccttctcg
     1261 gccgtgacgg tggccggcgg cggcaccatt tgcgctaaga aggatttgac cagcatcggc
     1321 ttgaccgcga actgccaggc gctcaacgcg ccgagcagga cggcaaccac aaggcccgtg
     1381 atgatcaaac gcctggtcat gttgctccca atccccgtgc cagccgaatg cggccccgcg
     1441 cggccgaaat tgaggccgat aggagaccgc tcatcgattg cagccggccc gcgccgcgag
     1501 gcagacgcgg acccctagcc agcacgggcc gcgcacgccg ctgtcaggaa aagaatgccc
     1561 ccggcgcccg ttcatcgctc gatcgttcct cgtgagatag gcgtgccgcg cgaccataca
     1621 agggtggccc tggcatgggc caagctggcg gcgtgccgag gcggtttgcc gcgccggccg
     1681 attgtgcatt cctgggcagc atagcaggcc tgcccaacgc gcgctcctgt aaatcataag
     1741 caagcttata ataagcctat gaaacggacc ctgaacccac ggcgcgagat cctattcgag
     1801 ctttccgacg ttgcccgcgc gctgcgcacc tacgtggatc aacgcgcgcg tgagcatggc
     1861 atgacccggg cacagtgggg ggtgcttgcc aggctggagc gcgatgaggg catggtgcaa
     1921 gccgaaatgg ccgaggcgct cgagatccag ccaatttcgc tggttcggct ggtggaccgg
     1981 ctgtgcgagc aagggctcgt cgagcggcgt gcgcacccga ccgatcggcg cgccaattgt
     2041 ctttacctta ccgaacaagg ccgcacccgc ttggcgcaga tggtgcccct ggggcgtgag
     2101 atctctggca atgtgcttgc cagcttcagc gagagtgatg tggcagagtt tctgagtaaa
     2161 ctgctatcgc tgaagcgcaa cattcgcagg gccgctgcgc gccgcaatgc gctgaacggc
     2221 ggcagcgagg tgcgtcatgc ccgctagcct cttcaatcgc atgcgcggcc tccagcgcct
     2281 gccgacgctg cgggccgtgc tgttgctgat catcccggcc ctcgtcggct gcttggcgct
     2341 cgccgtctat ctcttcagcg gccgctatgt cagcaccgat aacgcctata tcggggcgca
     2401 aaaggtgctg atcacgcccg aggtttccgg caaggtggtt agtatcgcgg tgcacgaagg
     2461 ccagctcttg cagccgggcg acgaactgtt ggccgtcgac cccgtgccct accgcctggc
     2521 ggcccaggaa gccgaggcca aactggcgcg cgtgaagacg gatttcgaaa atctcaagag
     2581 cagctttgcc agtctcggca agcagatcga gctttcacga caggccttgg aggccgcgca
     2641 atccgattac gaccgcaagc tggcgttatt gaacaatcgc atcagcacgc cctccgacgt
     2701 cgaccggtcg cgggcggcgc tggtggcggc caaggcgcag ctcgagcagc tgcagcagca
     2761 agaagccggc gcgcgcatcc aattgctcgg cgacgccaac cttcccctcg agcgcttccc
     2821 gcaatacgtg gaggcgttgg ccagccttga tcgtgcccgc cgcgacctga caaacacgac
     2881 cttgcgcgcg ccgatcgccg gggttgccac gcaggtcgcc agcatccaga tgggacgcta
     2941 tttggtggct ggcacggcgg tattcagcat catcgatagc gccaacgtct ggatcgacgc
     3001 caaccccaag gagaccgacc tcacctacgt tcggccgggc caacccgtcg acatcacggt
     3061 ggatgccttt ccatcccggc actggacggg cgtcgttggc gccatcagcc ctggcacggg
     3121 cgcgcaattt tccattctgc cgccccagaa tgccgccggg aactggatca agatcgtgca
     3181 gcgcgtgccc gtgcgcatcg aattcgcccc aggtcaggac ctgcgccgac tgcgctccgg
     3241 catgagcgcc atggtggaaa tcgacaccgg tcgggtgggc agaattgcct cgttcttcag
     3301 ctccagcgcg gtcgccgagg ggccagagga atgatcgccc gcggcggcag cacttttacg
     3361 ccggccctgc agcgcacgct gatcaccgtc tgcgtgatga tggccaccat catgcaggcg
     3421 ctcgacatta ccatcgccaa cgtagcgctg ccctacatgc agggcagcct gtcggcgacg
     3481 ctcgatcagg tcagctgggt gctcacctct tacatcgttg cggccgcgat catgaccgca
     3541 ccggtcggct ggcttgccgg ccgctttggc atcaagcgcg tgctggtgac ctgtgcggca
     3601 ggcttcacgg ccgcctccat gctgtgcg
//
LOCUS       DASTMT010000367         2447 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_2492593, whole genome shotgun sequence.
ACCESSION   DASTMT010000367 DASTMT010000000
VERSION     DASTMT010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2447)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2447)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2447
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2492593"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..331)
                     /locus_tag="VFR00_08585"
     CDS             complement(<1..331)
                     /locus_tag="VFR00_08585"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HEU0159357.1"
                     /translation="MIDLERASARALADAIRSKKVSSLEATQAAIGRLAAAHELTNCT
                     LSLDAEEALAAAKACDAAIAAGQPAGPLAGVPLAHKDMFDRKGKIASWGARIRAEAPA
                     AEDATVIA"
     gene            complement(328..531)
                     /locus_tag="VFR00_08590"
     CDS             complement(328..531)
                     /locus_tag="VFR00_08590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0159358.1"
                     /translation="MAEPSKDPREPAEAGPAAALEMSAERRRAAKAHAALLADTAAKV
                     AAELPLSADVDDFRRLLAAGARP"
     gene            complement(808..1251)
                     /locus_tag="VFR00_08595"
     CDS             complement(808..1251)
                     /locus_tag="VFR00_08595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012091943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1489 domain-containing protein"
                     /protein_id="HEU0159359.1"
                     /translation="MALHLIKLCVGCDSIEDLASWQAERLKQRRRAGEKRPNLFHRTF
                     QSPKRRDELLDGGSLYWVIKGIVQVRQPLLDITEGHKEDGSPCCLLILKNELIAVRPS
                     PRRAFQGWRYLGQAEAPPDLKRGAASGITAMPPKLRKQLAELGLL"
     gene            1418..2092
                     /gene="pncA"
                     /locus_tag="VFR00_08600"
     CDS             1418..2092
                     /gene="pncA"
                     /locus_tag="VFR00_08600"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018182251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HEU0159360.1"
                     /translation="MASLMPRAFAQSKITPGPDTALIVVDVQNCFTPGGSLAVNKGDE
                     IIPLINRLAKAFQNVVLTQDWHTAGHVSFASSHAGKKPFDSVQLAYGSQILWPDHCVQ
                     GTDGAAIHKDINIPHAQLIIRKGFNRNVDSYSAFVEADGRSKTGLDGYFNERGIKSVY
                     VVGLATDFCVAWTAMDARKLGFAAAVIEDACRGIDTQGSLAAAWKGMEQAGVKRLQSS
                     EIAVPG"
     gene            complement(2105..2428)
                     /locus_tag="VFR00_08605"
     CDS             complement(2105..2428)
                     /locus_tag="VFR00_08605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003604694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEU0159361.1"
                     /translation="MPKITFIEHDGTAHTVDAAIGDSVMETALGKSVPGIVAECGGSC
                     TCATCMVYVDEAWQDRVGARSAEEEDQLDFAFDVKPNSRLSCQIRVTEALDGLVVRTP
                     AYQGR"
BASE COUNT          388 a          813 c          802 g          444 t
ORIGIN      
        1 gcgcaatgac cgtggcgtcc tcagcggcgg gcgcctcggc gcggatgcgc gcgccccaac
       61 tggcgatttt gcccttgcga tcgaacatgt ccttgtgggc caggggcacg ccggcgagcg
      121 gcccggccgg ttggccggcc gcgatggcgg catcgcacgc cttggccgcg gcaagggcct
      181 cctcggcatc gaggctcaag gtgcagttgg tgagctcatg ggcggctgcc aggcgcccaa
      241 tggctgcctg cgtcgcctcc aggctggaga ctttcttgct gcggatggcg tcggcaagcg
      301 cccgcgccga agcgcgctcg agatcgatca tggccgcgcc ccggctgcga gcaggcggcg
      361 aaagtcgtcg acgtcggcgc tcagcggcag ttcggcggcg accttggccg ccgtgtcggc
      421 gagcagagcc gcgtgcgctt tggcggcgcg acgccgctcg gccgacattt ccaaggccgc
      481 ggccgggccg gcctcggcgg gctcacgcgg gtctttgctt ggctcagcca tgggcgaaac
      541 tcctttcggc aaggacggcc aagcctagcc ggagcgctcc gggcccacaa cagaaccctc
      601 tggggtgcag cggggcgccc tggggtgctc tcgggtgctc tcgggtgctc tggggtccac
      661 tgcaatcctc tcaggtcctc ttggatcatg ccttggacca ctctgggacc cttgggccac
      721 cgccccatgg cgtgctgagg ccaatgggct ggcgttgggg ccggcgcgcg cacgggtggc
      781 cggatcggcg gcagcgccag ggttgtctca gagcaagccc aattccgcca actgcttgcg
      841 tagctttggc ggcatcgccg tgatgccgct ggcggcgccg cgtttgaggt ccggcggcgc
      901 ttcagcctga cccaggtagc gccagccctg gaaggcacgg cgcgggctcg ggcggacggc
      961 gatcagctcg tttttcaaaa tcagcaaaca acaggggcta ccatcttcct tgtggccctc
     1021 ggtgatgtcg agcaatggct ggcgcacctg tacgatgcct ttgatcaccc aatagagcga
     1081 gccaccgtcg agcagctcgt cgcgccgttt ggggctttgg aaggtgcgat ggaacaggtt
     1141 tggccgcttt tcgccggcac ggcggcgttg cttgaggcgc tcggcctgcc aactcgccag
     1201 atcctcaatc gagtcgcaac cgacgcacag cttgatcaaa tgcaatgcca tggcgtcctc
     1261 gtggcggcct cgtcccgcca cagatttacc ggatccttgt gcttccggcg agtgagccgc
     1321 ccccttaagc tggggacagc accagcttgc cagaaggatc agcccgatgc acactttttc
     1381 ccgtcgctca tttcttgtcg gcacctgcgg cttaagcatg gcctcgctca tgcctcgcgc
     1441 cttcgctcaa agcaagatca cgcccgggcc cgatacggcc ttgatcgtgg tcgacgtgca
     1501 aaactgcttc acgcccggcg gcagccttgc cgtcaacaag ggcgatgaaa tcatccccct
     1561 tatcaaccga ttggccaaag ccttccagaa cgtggtgctg acccaggact ggcacaccgc
     1621 gggacacgtc tcgtttgcct cctcgcacgc cggcaagaag ccgttcgaca gcgtccagct
     1681 cgcctacggc agccagatcc tgtggcccga tcattgcgtg cagggcacgg acggtgctgc
     1741 catccacaag gacatcaata tcccccacgc ccagctcatc atccgcaagg gcttcaaccg
     1801 caacgtcgac agctattcgg ccttcgtgga agccgatggc cgttcgaaga cagggctcga
     1861 cggctatttc aacgagcgcg gcatcaaatc ggtctatgtc gtgggcctcg ccaccgattt
     1921 ttgcgtggcc tggacggcga tggatgcacg caagctcggc tttgccgccg cggtcatcga
     1981 ggatgcctgc cgcggcatcg atactcaggg ttcgctcgcc gccgcctgga aaggcatgga
     2041 gcaggccggg gtcaaacgcc tgcagtcgag cgagatcgca gtgccgggtt gaggccgcgc
     2101 agcatcagcg tccctgatag gccggcgtgc gcacgacgag gccgtcgagc gcctcggtca
     2161 ccctgatttg gcaggacagg cgcgagttgg gcttgacgtc gaaagcgaag tcgagctgat
     2221 cctcctcctc ggcgctgcgg gcacccacgc ggtcctgcca agcctcgtca acatagacca
     2281 tgcaggtggc gcaggtgcac gagccgccgc attcggcgac gatgcccggc acgctcttgc
     2341 ccagcgccgt ttccatgacg ctgtctccga tcgcggcatc gaccgtatgc gcggtgccgt
     2401 catgctcgat gaaggtgatt ttcggcattg caggcgttgc ggttgtc
//
LOCUS       DASTMT010000368         2308 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_1187236, whole genome shotgun sequence.
ACCESSION   DASTMT010000368 DASTMT010000000
VERSION     DASTMT010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2308)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2308)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2308
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1187236"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(220..2052)
                     /gene="recQ"
                     /locus_tag="VFR00_08610"
     CDS             complement(220..2052)
                     /gene="recQ"
                     /locus_tag="VFR00_08610"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR01389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HEU0159362.1"
                     /translation="MPQDGLDTARDVLRRTFGHADFLGLQAGVIGEILAGRSALAVLP
                     TGGGKSLCYQIPALVRPGLGLVVSPLIALMADQVSALQQSGVAAEKLDSNTDLDMRGD
                     IWRRIDQGKLHLLYLSPEGLMQPSMLERLSHLPLALVAIDEAHCVSQWGHDFRPEYRM
                     LGRLAEVFPEVPRLAVTATADARTRGDIRAELRLADALEFVDCFARPELALSAERKRG
                     KGHGRVLELVAERPKRSGVIYAGSRDGVDKLSQKLVAEGVPALAYHAGLDKDVRAARL
                     EQFLEAEAAVMVATIAFGMGVDKPDIRFVIHADPPASIEAYWQEIGRAGRDGAPAEGI
                     TLYSASDLAWALRRIDSREVDNTVKTVQARKVRQLYAMLDGTSCRAAAVRRYFGETAV
                     EACGQCDLCLNPPEALDVTVAAQKALSAVHRLGGRFGRGRIVDHLLGKTKDALASETQ
                     LSTFGIGREYSAASWRDLLDQLQFEGLLREDPNDGRPLIGLGEADGVRAVYRGERRIS
                     MRQMTAVGEPAGRKERARKRHGGEDVGVGGADAPLFEALRAWRRERAAEQHVPPYVIF
                     HDSTLSAIARLRPASLDDLSKVSGVGQSKLKRYGTDVLEVVRAN"
BASE COUNT          416 a          774 c          735 g          383 t
ORIGIN      
        1 tcggctccct tattggaccc gcgcggcagc ctcgtgggct caaggactac agaactaatg
       61 gccatcatgg ggtgatcagc gcaaactcaa cggacagaaa ttctgtgcag catgaggtcg
      121 gcaggaatac ggcgcataca agtcgggggg catcacgata gcggcgcggt cactcccgct
      181 gcctggcctc gcatgcaatc acgccacggt cgcggggcct cagttcgccc gcacgacctc
      241 cagcacatcg gtaccataac gcttgagctt gctctggccg acgccgctga ccttcgatag
      301 gtcatcgagg ctggcaggcc gcaaacgggc gatggccgac aaggtgctat cgtgaaaaat
      361 gacgtagggc ggcacgtgct gctctgcggc gcgctcacga cgccaggcgc gcagagcctc
      421 aaacagcggg gcatccgctc cgcccacccc cacatcttca ccgccatgac gcttgcgcgc
      481 acgctccttc cgcccggctg gctcccctac ggccgtcatc tgacgcatgg agatgcgccg
      541 ctcgccgcga taaacggctc tgaccccgtc agcctcaccc aagccgatca gggggcgccc
      601 gtcattgggg tcctcgcgca acaggccctc gaactgcagc tggtccagca ggtcgcgcca
      661 gctggcagcg ctgtactcgc gtccgatgcc gaacgtcgac agctgggtct cagacgccag
      721 ggcatccttg gtcttaccca gcaggtggtc gacgatccgg ccgcgaccga accggccgcc
      781 caggcggtgc acggcggaga gcgctttctg cgccgccaca gtgacatcaa gggcctctgg
      841 cggatttagg cagaggtcgc actggccgca ggcctcgacc gcggtctcgc cgaagtagcg
      901 gcgcacggcg gccgcgcgac agctcgtgcc atcgagcatg gcatagagct ggcgcacctt
      961 gcgcgcctgc acggtcttga ctgtattgtc gacctccctg ctgtcgatac ggcgcaacgc
     1021 ccaggcgagg tccgaggcgg aatagagagt gatcccctcc gccggggccc catcgcgccc
     1081 ggcacggccg atttcctgcc agtaggcctc gatagatgca ggcggatcgg cgtggatgac
     1141 aaagcgaatg tctggtttgt cgacacccat gccgaaggcg atggtcgcca ccatcaccgc
     1201 cgcctcggcc tccagaaact gctccagccg cgccgcgcgc acgtctttgt cgaggccggc
     1261 gtgataggcc agcgccggca ctccctcggc gaccagcttc tggctgagct tgtcgacccc
     1321 gtcgcgcgag ccggcataga tcaccccgga ccgtttcggt cgttcggcca ccagttcaag
     1381 cacgcgcccg tgccccttgc cgcgcttgcg ctctgccgac agggcgagct ccgggcgagc
     1441 aaagcagtcg acaaattcca gcgcatcagc cagccgcagc tcggcgcgga tgtccccacg
     1501 tgtgcgggca tcggcggtgg cagtaaccgc caggcgcggc acttcaggga atacctcggc
     1561 caaccggcca agcatgcggt attcggggcg gaagtcgtgg ccccattggc tgacgcaatg
     1621 ggcctcgtcg atggctacaa gtgcaagcgg gaggtgcgag aggcgctcca acatcgaagg
     1681 ttgcatcaag ccttcgggcg acaggtagag caggtgcagt ttcccttgat cgatccgccg
     1741 ccagatgtcg ccgcgcatat ccaagtcggt attggagtcg agcttctcgg ccgccacccc
     1801 cgactgctgc aaggccgaca cctggtcggc catcagggca atcaggggtg agacaacaag
     1861 accgagccca ggccgaacca aagcgggaat ctggtagcag aggctcttgc cgccgccggt
     1921 gggcagtacg gcgagcgcgc tgcgtccggc cagaatttcg ccgattaccc cggcctgcag
     1981 cccgaggaag tcggcgtggc cgaaggtgcg gcgcagtacg tcccgagcgg tgtcgagtcc
     2041 atcttggggc atgagcgatg ccatggaagt tgaaatgcgc gtatcagcca ctaccgggga
     2101 tagatagact gcaccatcgc gagtgccgcc aattgagcct gaacggcaat gtcgttcgta
     2161 ccttctattg agctgccagt tggcgcttga gcttgtgcac cgtgcctgtt ccaagcccag
     2221 tcagtcgcgc cactttgccg atgccgatgc ccttagcaag ttcggcatga gcgcgggcaa
     2281 tctgtttggg ctcggcgccc ggtcgacc
//
LOCUS       DASTMT010000369         3308 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_2493303, whole genome shotgun sequence.
ACCESSION   DASTMT010000369 DASTMT010000000
VERSION     DASTMT010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3308)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3308)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3308
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2493303"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1287
                     /locus_tag="VFR00_08615"
     CDS             <1..1287
                     /locus_tag="VFR00_08615"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011501235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HEU0159363.1"
                     /translation="RIGTEHEKFLFHTDTLGPVPYAGPRGVRSLMEGLISRYGWVPIM
                     EGDNIIALKRPSGEVNGGTVSLEPGGQFELSGAPLQTVHELGAETQLHLDQVRAVGDA
                     LGIGILGVGFSPNWTFEATPRMPKQRYGVMSRYMPLVGSRGLDMMYRTATIQVNLDFA
                     DEADMVKKLRVSLALQPIATAIFACSPFTEKKPNGFLSLRSEVWRHTDHARTGMLPFA
                     FEPGMGFERYATYALEVPMYFVYRDGRYIDVAGASFRDFLEGRLSALPGERPTLDDWS
                     DHLTTLFPEVRLKQFLEMRGADGGRWRRICAVAAFWTGLLYDAQALEAAWDLVKPWSA
                     ADRQGLRDQVPRLALAAPVHGTTAWQIARQATALAHAGLARRARHNNKGQDETIFLEP
                     VEAIIKGGLTPAQEMLERYETVWSRSTDPLFTEFAF"
     gene            1407..1763
                     /locus_tag="VFR00_08620"
     CDS             1407..1763
                     /locus_tag="VFR00_08620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0159364.1"
                     /translation="MAGAHGQGDAARPVRLPGRCAALWLGLGLLALALTGPAAAQNAC
                     SSGCRTAYGACYKSTHDRSRCQVQLQRCLEGCIRSRESTRRRSSLSGGSRAPIPFGRA
                     TGQFGHVQAHSAKLFK"
     gene            2054..>3308
                     /locus_tag="VFR00_08625"
     CDS             2054..>3308
                     /locus_tag="VFR00_08625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEU0159365.1"
                     /translation="MSRVILPEPDRAILARRESIVADLARLVGSQAIIADEIGRRAYE
                     TDALTAYRKLPLAVVLPSTTEEVSRVLSYCHRWGIKVVARGAGTSLSGGALPAEDAVV
                     ITVSRMNRVLEIDYVNRVARVEVGITNLAISEAVNAGGFFYAPDPSSQLACTLAGNIG
                     MNSGGAHCLKYGVTTNNVLGLKLVLVDGSIVELGGPHLDAPGYDLLGLIVGSEGQFGI
                     VTEATVRILRAPEGARPMLIGFNSSEAAGQCVAAIIGSGIIPVAIEFMDRPAIRVCEA
                     FAKAGYPDVEALLIVEVEGSEQEIKALLDSITELAKPFDPRELRVSQSAEQSAAIWKG
                     RKSAFGAIGRISDYYCMDGTIPLGKLAHVLVEISRICDTHGLKVANIFHAGDGNLHPL
                     ILYDANNAAEAEKAERAGAEVLELCV"
BASE COUNT          522 a         1084 c         1132 g          570 t
ORIGIN      
        1 cgcatcggca ccgagcacga gaagtttttg ttccataccg atacgctggg cccggtgccc
       61 tatgccggac cgcgcggggt ccgcagcctg atggaaggct tgatcagccg ttacggctgg
      121 gtccccatca tggaaggcga caacatcatc gcgctcaagc gcccgagcgg cgaggtcaac
      181 ggcggtaccg tctcactcga accgggcggc caattcgagc tgtccggggc accgttgcag
      241 acggtgcacg agctgggtgc ggaaacgcag ctgcatcttg atcaggtacg cgcggtcggc
      301 gatgcgctcg ggatcggcat tctcggcgtc ggtttctcgc ccaattggac attcgaggcg
      361 acgccgcgca tgccgaagca gcgttacggc gtgatgagcc gctacatgcc gctcgtgggc
      421 tcgcgcggcc tcgacatgat gtaccgcacg gccaccatcc aggtgaattt ggacttcgcc
      481 gacgaggcgg acatggtcaa gaagctgcgc gtgtcgctgg ctttgcagcc gatcgccacg
      541 gccatcttcg cctgttcccc gtttaccgag aagaaaccga acgggttttt gtccttgcgc
      601 agcgaggtgt ggcgccacac cgatcacgcc cgcaccggca tgttgccgtt cgccttcgag
      661 cccggcatgg gctttgagcg ctacgcgacg tacgcgctcg aggtgccgat gtatttcgtc
      721 taccgcgacg ggcgctatat cgacgtcgcc ggcgcctcgt tccgggattt tctggagggc
      781 cggctgtccg ccctgccggg cgagcggccc acgctcgatg attggtccga tcacctcacc
      841 acgctgttcc ccgaggtgcg tctcaagcag ttcctggaaa tgcgcggcgc cgacggcgga
      901 cgctggcgac gcatttgcgc cgtggccgcc ttctggaccg gcttgctcta tgatgctcaa
      961 gccctcgagg cggcctggga cctggtcaag ccctggagcg cggcggaccg ccaggggctg
     1021 cgcgaccagg tgccgaggct ggcgctcgcc gccccggtcc acggcaccac ggcctggcag
     1081 atcgcgcgcc aagcgacggc gctggcgcat gccgggttgg cgcgacgggc ccgccacaac
     1141 aacaagggcc aggacgagac aatttttctg gagcccgtcg aggccatcat caagggcggc
     1201 ttgacgccgg cccaggagat gctcgagcgc tacgagacgg tttggagccg gagcactgat
     1261 ccactgttta ctgagtttgc attttaggcc ggacggcgcc ccgcaggggc gccgaggtgc
     1321 cggccgagcc cacgccacat tcaggtgacg ttcatgcttt cgagcgtagc aatgcgccga
     1381 tgcggctcac tttacgtcga caggggatgg ccggcgcaca tgggcagggc gacgcggcca
     1441 ggccggtccg cctgcccgga cgttgcgccg cgctgtggct ggggctcggc ttgctggcgc
     1501 tcgcgctgac ggggccggcg gcggcgcaga atgcctgctc gtcgggctgc cgcaccgcct
     1561 atggggcctg ctataagtcg acgcacgatc gctcgcgctg ccaagtgcag ctgcagcgct
     1621 gcctcgaggg ctgcatccgc agtcgcgaat cgacgcggcg ccgctcctcg ctgtcgggtg
     1681 gcagccgtgc gccgatcccg ttcggccgcg ccacgggcca gttcggccac gtccaggccc
     1741 attcggcgaa gctcttcaaa tagaacgacg cggccccccg ccagcggcga aagcgggcgg
     1801 ccacgccgcc gtgcaggggt cgtggcgacc tccccttggg gttgcggcag gccggctggc
     1861 gcagcaggcc gaccttgcgg gggcgccggc cgccaagtcg ccgcggcgcc agcagcttta
     1921 cgggcagcgc gatctcctcg ccggccgtgc gttgagctgc cacgggacaa gcgcccaagt
     1981 gtcatggcgg cagagggggg ttgcggctat actttggccc gcgactcttg gcgtgctgga
     2041 gagcaagcaa cccatgtccc gcgtgatcct gccggagccc gaccgcgcga tcctcgccag
     2101 gcgcgagagc atcgtggcgg atttggcgcg cctggtcgga tctcaggcca ttatcgccga
     2161 tgaaattggc cggcgcgcct acgagaccga tgcgctgacc gcctaccgca agctgccgct
     2221 ggcggtcgtg ttgcccagca cgacggagga ggtgtcgcgc gtccttagct actgtcatcg
     2281 ctggggcatc aaggtggtgg cgcgcggcgc cggtacctcg ctgtcgggcg gcgccttgcc
     2341 ggccgaggac gccgtggtga tcaccgtctc gcgcatgaac cgcgtgctcg aaatcgacta
     2401 cgtcaatcgg gtggcgcgcg tggaggtcgg catcaccaat cttgccatca gcgaagcggt
     2461 caatgccggc ggcttcttct acgcgccgga tccgtcgagc cagctggcct gcacgctggc
     2521 cggcaacatc ggcatgaact cggggggcgc gcactgcctc aaatatggcg tgacgaccaa
     2581 taacgtgttg ggcctcaagc tggtgctcgt cgatggcagc attgtcgagc tcgggggtcc
     2641 gcatctcgat gctcccggct acgatctttt gggcttgatc gtaggctcgg agggccaatt
     2701 cggcatcgtc acggaagcaa cggtgcggat tttgcgcgcc cccgagggcg cccggccgat
     2761 gctgatcggc ttcaattcca gcgaggccgc cggccaatgc gtggccgcca ttatcggctc
     2821 gggtattatt cccgtggcca tcgaattcat ggaccgaccc gccatccgcg tgtgcgaggc
     2881 cttcgccaag gcgggctatc ccgacgtcga agcgctgctg atcgtcgagg tggagggctc
     2941 cgagcaagag atcaaggcgc tgctcgacag catcaccgag ctcgccaagc ccttcgatcc
     3001 gcgcgagttg cgcgtgagcc agagcgccga acagagcgcc gccatctgga aaggccgcaa
     3061 gtcggcgttc ggggcgatcg gccgcatctc cgattactac tgcatggacg gcaccattcc
     3121 gctcggcaag ctggcgcacg tgctggtgga gatctcgcgc atttgcgaca cgcatgggct
     3181 gaaggtcgcc aatatcttcc acgccggcga cggtaacctc catcccctca ttctctatga
     3241 tgccaacaat gcggcagagg ccgagaaggc ggaacgggcg ggtgcagagg tgctggagtt
     3301 atgcgtgg
//
LOCUS       DASTMT010000370         1755 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_6009896, whole genome shotgun sequence.
ACCESSION   DASTMT010000370 DASTMT010000000
VERSION     DASTMT010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 1755)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1755)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1755
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6009896"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..235)
                     /locus_tag="VFR00_08630"
     CDS             complement(<1..235)
                     /locus_tag="VFR00_08630"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598726.1"
                     /note="Catalyzes the reversible hydrolysis of the amide
                     bond within dihydroorotate. This metabolic intermediate is
                     required for the biosynthesis of pyrimidine nucleotides;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEU0159366.1"
                     /translation="MRKSDPTPSPDATVFLNARLIDPASGKDEPGGLLVRGGLIADLG
                     THLRRNAPDGAAVIDCKGHVLCPGIIDLQVFTGE"
     gene            complement(250..1200)
                     /locus_tag="VFR00_08635"
     CDS             complement(250..1200)
                     /locus_tag="VFR00_08635"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007603315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEU0159367.1"
                     /translation="MTSAIHPASFVRRHLLTCESLKAGEINALLDLADKAAEGNRQVI
                     KKRDVLRGRTLINLFFEASTRTQSSFELAGKRLSADVMNMNVSTSSVAKGETLIDTAM
                     TLNAMRPDILVVRHHAAGAVELLAQKVDCSVINAGDGAHQHPTQALLDALTIRRAKGR
                     IAGLTVAICGDILHSRVARSNINVLNTMGARVRVVAPSTLLPAHADRLGAEVFTDMNA
                     GPEDADIVMMLRLQKERMQGSYVPSTREYFHFFGLDHEKLARAKPDALIMHPGPMNRG
                     VEIASTIADHSRSVIREQVEMGVAVRMAVLEAMARLLPNH"
     gene            1339..>1755
                     /locus_tag="VFR00_08640"
     CDS             1339..>1755
                     /locus_tag="VFR00_08640"
                     /inference="COORDINATES: protein motif:HMM:NF036723.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation response protein"
                     /protein_id="HEU0159368.1"
                     /translation="MLEPFQTHEVFNQSPPFQDVNLFTTDTALLEAVEREGAAAAVPQ
                     LQHFGQTCGSAEALEQARLANENPPRLLRFDATGERLDVVEFHPAYHALMAISIGQGL
                     HASAWDHLASGQTKRRAGASVERSAGCYLANQMEAGH"
BASE COUNT          301 a          550 c          609 g          295 t
ORIGIN      
        1 gttcgcccgt gaacacctgc aggtcgataa taccggggca caacacgtgt cccttgcagt
       61 cgatcacggc cgccccatcc ggggcgttgc gccgcaggtg ggtgccgaga tcggcgatca
      121 gcccgccgcg caccaacagg ccgcccggtt cgtccttgcc cgaagccgga tcgatgagac
      181 gcgcgttgag gaagacggtc gcgtccggtg aaggcgtcgg atcggatttg cgcatacgct
      241 cgtgacttct caatggttgg gcaggaggcg cgccatggcc tcgagcaccg ccatgcgcac
      301 ggccacgccc atttcgacct gttcgcggat gacgctgcgg gagtggtcgg caatggtgga
      361 ggcaatctcg acgccgcggt tcatcgggcc cgggtgcatg atcagggcgt cgggcttggc
      421 gcgcgccaac ttttcgtgat cgaggccgaa gaagtggaag tactcgcgcg tcgacggcac
      481 ataggagccc tgcatgcgct ctttttgcag gcgcagcatc atgacgatgt cggcgtcttc
      541 cgggccggcg ttcatgtcgg tgaagacctc ggcgccgagc cggtcggcgt gggcgggcag
      601 cagtgtcgag ggcgccacga cgcgcacccg tgcccccatg gtgttgagca cgttgatgtt
      661 ggagcgggcg acgcgcgagt gcagaatgtc gccgcaaatg gcgaccgtca gaccggcaat
      721 gcggcccttg gcgcgacgga tggtgagggc gtcgagcaga gcctgagtgg gatgctggtg
      781 cgcgccgtcg ccggcgttga tgaccgagca atcgaccttc tgggccaaca gttcgaccgc
      841 ccccgcggcg tggtggcgca ccaccaggat atccggccgc atggcgttga gcgtcatggc
      901 ggtgtcgatc agcgtctcgc ccttggccac cgaggaggtg gaaacgttca tattcatgac
      961 gtcggcgctc agccgcttgc cggcgagctc aaaggagctc tgcgtgcgcg tcgaagcctc
     1021 gaaaaacagg ttgatcagcg ttcgaccgcg cagcacgtcg cgctttttga tgacttgccg
     1081 gttgccctcg gcggccttgt cggccaaatc caagagggcg ttgatttcgc cggccttgag
     1141 gctttcacaa gtcaacaaat ggcggcggac gaaggaggcg ggatgaatgg cgctggtcat
     1201 taaaggcggt cctatacgcc gactccggcg ggctcgcaag ccgcaccagg acggtggcgg
     1261 tggagaaggt ctgaggcggg ctgcgccctg tgctggcagt tgctaggctg aggctttcct
     1321 cgagaggcgc aaacccccat gctcgagccc ttccagacgc atgaagtttt caaccagagc
     1381 ccgccgttcc aggacgtgaa cctgttcacg accgacacgg ccctgcttga ggccgtcgag
     1441 cgcgagggcg ccgcggcagc ggtgccacag ctgcagcact ttgggcaaac ctgcggcagc
     1501 gccgaggccc ttgaacaggc tcggctcgcc aacgaaaacc cgccgcggct gttgcgcttc
     1561 gacgccacag gcgaacgact ggacgtggtc gagttccacc cggcctatca cgccctgatg
     1621 gccatcagta tcgggcaagg cctgcacgcc tcggcctggg accatctcgc cagcggccag
     1681 acgaagcggc gcgcgggggc cagcgtggag cgctcggccg gctgctatct cgccaaccag
     1741 atggaagcgg gccac
//
LOCUS       DASTMT010000371         3789 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_2824596, whole genome shotgun sequence.
ACCESSION   DASTMT010000371 DASTMT010000000
VERSION     DASTMT010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3789)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3789
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2824596"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..519
                     /locus_tag="VFR00_08645"
     CDS             <1..519
                     /locus_tag="VFR00_08645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018261717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HEU0159369.1"
                     /translation="AIYGGDAGVTLLEAACGERPGAVMLRVNSANPTVSTASAEFVAA
                     AREAGGWEGQVWDRTIEVPSTTLDQLLAEHGVPAFAKIDVEGFEAAVLAGLSRPLPAL
                     SFEFTTIQRDVAYRCLDRLVSLGRYGCDIALGESQSLTFDRWLSPTEMAAHIATLTHD
                     ANSGDVYCVLNA"
     gene            512..1570
                     /locus_tag="VFR00_08650"
     CDS             512..1570
                     /locus_tag="VFR00_08650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0159370.1"
                     /translation="MRDRRASVGEVLAMAAGVRQGDVKARAGASARRVALAVALGLAG
                     LARGPLGASAEPLAVEVANASEPVLCAERDNVTLNLTSSAVRSFRITALHPTYMGSLQ
                     RDSFAADWTACTAPTDPRAGPPRPPKRKTLYEDIEMWVVGLTFQDFWRPATATVRVGE
                     HSEKDIHLLQVWMIRPMGGEEVLVFYPQDGNWRLRPKAPDNRAPTAFGSSVLIGPVEL
                     DGRPLVKINEVVFDPKRRSFRLSFARGGAASVRLGEIDETQHALEVAFEQPIVGLPFA
                     ALRSMYVTDVNNDVARVAVRETGASGWREAGIMAFTGARANELWAGRVTPSRHNTSAP
                     DLLFSLFSEKSDGASARK"
     gene            complement(1574..2440)
                     /locus_tag="VFR00_08655"
     CDS             complement(1574..2440)
                     /locus_tag="VFR00_08655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01459 family HAD-type hydrolase"
                     /protein_id="HEU0159371.1"
                     /translation="MPTLILDAAGPLLDRYDAIFCDVWGVVHNGEKAYVPAGEALTRF
                     RQQGGTVVLVSNAPVPADGVEQVLAKTGVRRDAWDVIVSSGDIALKHIAEKGYKRMHW
                     VGPSVRDYKLYVRLREPQSALEDADAIVCTGLKDDRRETVEDYRHLIAAGLQRGLPFV
                     CANPDLVVDVGELRLPCAGSIAAEYERGGGNVFWAGKPHPAAYRTALQRAAEVAGTMP
                     ALDRILAIGDTVRTDMAAARGLGIDGLLIAAGIHRNDLLVGGQIDPARLASLLAIPEA
                     STTVAAMAELRW"
     gene            2527..3525
                     /locus_tag="VFR00_08660"
     CDS             2527..3525
                     /locus_tag="VFR00_08660"
                     /inference="COORDINATES: protein motif:HMM:NF016908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HEU0159372.1"
                     /translation="MMHLSYAQNMEDYHLELIFAGVDDGAYVDVGAGHPVADNVSFHF
                     YLKGWRGLVVEPQEALARLYAHVRPRDHAVACLAGSAAAEIDFYVVDKLHGFSSTVHA
                     NAQGAGQFGASYHTIRKPVQPLSALIDAAGLTGIDFLKIDVEGAEGEVLAGLDFGRHR
                     PRVLLIEAVAPGSMAEAWGEWEPVLLGNGYHFAFFDRLNRFYVAEEAKPLLARFPKEP
                     ARWDGVQHLWDLGRAPLRPDHPDHELAQLLLHGFLAELPALAPEVLATLLARGLSATG
                     KGDLTGPGLAALIAGAARPGGAARQDPELAALLNSDAFRAALGRIASTYDGGHLMD"
     gene            complement(3539..>3789)
                     /locus_tag="VFR00_08665"
     CDS             complement(3539..>3789)
                     /locus_tag="VFR00_08665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016847148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEU0159373.1"
                     /translation="RAFLFNTAWRDSRNENVSKEVSMLKALAGTLVNKVMYDCLQFHG
                     GMGYMRESAIERMTRDARVQAIGGGATEVMLDEVAKRL"
BASE COUNT          602 a         1248 c         1295 g          644 t
ORIGIN      
        1 gccatatatg gcggcgatgc gggcgtcacg ctgctggagg ccgcatgcgg ggagcggccc
       61 ggcgccgtga tgctgcgcgt caactcggcg aatccgactg tctcgacggc ctcggccgag
      121 ttcgtcgccg ccgcgcgcga agccggaggc tgggagggac aggtgtggga tcgcacgatc
      181 gaggtgccct cgaccacgct cgatcagctg ctcgccgagc acggcgtgcc ggcattcgcc
      241 aagatcgatg tcgaggggtt cgaggcagcg gtgctcgccg gcctctctcg gccgctgccg
      301 gcgctctcct tcgaattcac cacgatccag cgcgacgtcg cctatcgctg cctcgatcgg
      361 ctagtctcac ttggccgcta cggctgcgat atcgctctcg gcgaaagcca gagcttgacg
      421 tttgatcgct ggctgagccc gaccgaaatg gccgcccaca tcgccaccct gacccacgat
      481 gccaactccg gggatgtcta ttgtgtgctc aatgcgtgat cgccgcgcga gcgttggcga
      541 ggtcctggca atggccgcag gcgtgcggca gggcgacgtc aaggcccggg cgggcgcaag
      601 cgcccggcga gtggcgctcg ccgtcgccct cggcctcgcc ggccttgccc gcgggccgct
      661 gggcgcgtcc gccgagccgc tcgccgtcga ggtggccaat gccagcgaac cggtgctgtg
      721 cgccgaaagg gacaatgtca ccttaaacct cacctcgagc gcggtgcgca gctttcgcat
      781 caccgcgctg cacccgacct atatgggcag cctgcagcgt gacagctttg cggcggattg
      841 gacggcctgc acggcgccca ccgatccgcg cgccggtccg ccgcggccgc ccaagcgcaa
      901 gaccctctac gaagacatcg agatgtgggt ggtgggtttg acctttcagg atttttggcg
      961 tccggccacg gcgacggtgc gcgtcggcga gcacagcgag aaggacatcc atctcctgca
     1021 ggtgtggatg atccggccga tggggggcga agaggtgttg gtgttctatc cccaggacgg
     1081 caattggcgt ctgcgtccca aagcgccaga caatcgcgcc cctacggcgt tcggctcctc
     1141 ggtgctgatc ggacccgtcg agcttgacgg tcggccgctc gtcaaaatca acgaggtggt
     1201 gttcgatccc aaacgccgca gtttcaggct cagctttgcg cgcggtgggg cggccagcgt
     1261 gcggctcggc gagatcgatg aaacgcagca tgccctcgag gtcgccttcg agcagcccat
     1321 cgtcgggttg cccttcgccg ccctgcgctc catgtacgtg accgacgtca acaacgacgt
     1381 ggcgcgcgtc gccgtgcgcg agacgggggc gagcggttgg cgcgaagcgg ggatcatggc
     1441 cttcacgggg gcgcgggcga acgagctctg ggccggccgg gtgacgccct cgcgccacaa
     1501 cacgagcgca cccgatctgc tgttctcgct gttcagtgag aagagcgacg gcgcgtcggc
     1561 acgcaagtag gcgctaccat ctgagctcgg ccatggcggc cacggtggtg gacgcttccg
     1621 ggatggcgag caggcttgcc agccgtgccg gatcgatttg gccgccgacg agcaggtcgt
     1681 tcctgtgaat gccggccgca atgaggaggc catcgatgcc caggccgcgc gccgccgcca
     1741 tgtcggtgcg cacggtatca ccgatggcaa gaatgcggtc gagggccggc atggtgccgg
     1801 cgacctcggc ggcccgctgc aacgcggtac ggtaagcggc ggggtgcggc ttgccggccc
     1861 agaacacgtt gccgccaccg cgctcgtatt cggcggcgat gctgccggcg cagggcagcc
     1921 gcagctcccc cacgtcgacg accaggtcgg gattggcaca gacaaagggc agcccgcgtt
     1981 gcaagccggc cgcgatcagg tggcgatagt cctcgaccgt ctcgcgacgg tcgtccttga
     2041 gcccggtgca gacgatggca tcggcatcct cgagcgcgct ctgaggttcc ctgaggcgca
     2101 cataaagctt gtagtcgcgc acgctgggtc ccacccaatg catgcgctta taaccctttt
     2161 cggcgatgtg cttaagcgcg atatctcccg aggagacgat cacgtcccag gcatcccggc
     2221 gcacaccggt tttggccagc acctgttcga caccatcggc cggcacggga gcattggaga
     2281 cgagcacgac cgtgccaccc tgctggcgga agcgcgtcag cgcctcgccg gccggcacat
     2341 aggctttctc gccgttgtgc acgacccccc aaacgtcgca gaaaatggcg tcgtagcggt
     2401 ccaacaacgg accggccgcg tcaaggatga gggttggcat ggatcggggc gaggcctcgg
     2461 cgctgctctc gacgaaacgg acgggcgcca ctagaagcag tgagccgccg caacacaacg
     2521 ggcgaaatga tgcacctgtc ctacgcgcag aatatggagg attaccacct cgagctgatc
     2581 tttgccggcg tggacgatgg cgcctatgtc gatgtcgggg ccggccatcc cgttgccgac
     2641 aatgtgtcgt ttcatttcta cctcaagggc tggcgcgggc tggtggtgga gccgcaggag
     2701 gcgctggccc gcctttatgc tcacgtccgc ccgcgcgacc atgccgtcgc ctgcctggcg
     2761 ggcagtgccg cggccgaaat cgatttttac gtggtcgaca agctgcacgg attctcctcg
     2821 accgtacacg caaacgccca aggcgcgggc cagttcggcg ccagctacca caccattcgc
     2881 aagcccgtgc aaccgttgag cgcgttgatc gacgcggcgg ggctcacagg catcgatttc
     2941 ttgaagatcg acgtcgaagg tgccgagggc gaggtgctgg cgggcctcga tttcgggcgc
     3001 caccgtccgc gcgtgctgct gatcgaggcg gttgcgccgg gcagcatggc cgaggcctgg
     3061 ggggagtggg agcccgtgct gctggggaac ggctaccact ttgccttctt tgacaggctc
     3121 aaccgctttt acgttgccga ggaggcaaaa ccattgctgg cgcgcttccc caaggaaccg
     3181 gcgcgctggg acggcgtgca acacttgtgg gacctcgggc gggcgccgct acggcccgac
     3241 catcccgacc acgagctggc gcagctgctg ctgcacggtt ttttggccga attgccggcc
     3301 ttggcccccg aagttctcgc aaccttgctc gcgcgcggtc tgtcggcgac gggcaagggg
     3361 gatttgacgg ggccgggcct ggcggccctc attgccggcg cagcaaggcc gggcggcgcg
     3421 gcccgtcagg accccgaact ggcggcgctc ttaaacagcg atgcgtttcg cgccgccctg
     3481 gggcgcattg cttctaccta cgacggcggt cacttgatgg actgagttcg gcatcggctc
     3541 aaaggcgttt ggcgacctcg tccagcatca cctcggtggc gccgccgccg atcgcctgca
     3601 cgcgggcatc ccgcgtcatg cgctcgatgg cgctctcgcg catgtaaccc atgccgccgt
     3661 ggaattgcag gcaatcgtac atcactttat tgacgagggt gccggcgagc gccttgagca
     3721 tcgacacctc cttgctgacg ttctcgttgc ggctatcgcg ccaggcggtg ttgaacagga
     3781 aggcccgcg
//
LOCUS       DASTMT010000372         7170 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_1514146, whole genome shotgun sequence.
ACCESSION   DASTMT010000372 DASTMT010000000
VERSION     DASTMT010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 7170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7170)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7170
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1514146"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..209
                     /locus_tag="VFR00_08670"
     CDS             <1..209
                     /locus_tag="VFR00_08670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016552623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2'-deoxycytidine 5'-triphosphate deaminase"
                     /protein_id="HEU0159374.1"
                     /translation="FGHAAAGGAGAKAVLEVRSHKVPFILEDAQIVGRLIYERVTEVP
                     EVLYGASLQSNYQGQGLKLSKHFR"
     gene            398..1030
                     /locus_tag="VFR00_08675"
     CDS             398..1030
                     /locus_tag="VFR00_08675"
                     /inference="COORDINATES: protein motif:HMM:NF024579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0159375.1"
                     /translation="MKRTSALIGIVLGLALATAARADSAADCFSEDLDRRIEGCTALI
                     QQGDQGAADLSYAYAMRALAYSLKGRYETAIRDYDVAIAMKPDFAVALNNRAWALFRW
                     GKASEGLPDVERSLELSPTSAHSLDTRAHIKQSLGDPKAALRDYDRAMWYGGARMIKL
                     YQCGLTEQKLYTGEIDGTWRPEVTKALEQCVLDKACDPLPADEQCRAATS"
     gene            1193..2293
                     /locus_tag="VFR00_08680"
     CDS             1193..2293
                     /locus_tag="VFR00_08680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019196040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEU0159376.1"
                     /translation="MKIAQIAPPMESVPPRLYGGTERIVSYLTEELVALGHDVTLFAS
                     GDSITRAKLIASCPQALRLNPAVRDPIPYYMMMLSKVRRAAAEFDILHFHIDQFHFPL
                     FEDMAARVVTTMHGRQDLPDLQNLYAGFPSMQLVSISEAQRRPLTGASFAATVYHGLP
                     RDLLSPSLVPRGGYLAFLGRISPEKRVDRAIAIARAVGVPIKIAAKIDRVDEDYFHAE
                     IEPLLNQPGVEYIGEIDDRKKSRFLSDARALLFPINWPEPFGLVMIEAMACGTPVLAF
                     EAGAVREIIDEGVTGHIVRSVEEGICKIGAVMALDRGRVRRRFEERFTATRMANDYVK
                     VYQALLGEEARARPGWEPAEVASVAEPVAAVH"
     gene            2331..4520
                     /locus_tag="VFR00_08685"
     CDS             2331..4520
                     /locus_tag="VFR00_08685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009798316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching N-terminal
                     domain-containing protein"
                     /protein_id="HEU0159377.1"
                     /translation="MVVSHPAKPSAPASAPTEAPFYIPATNSPSRPRRTLKHDDTFAV
                     FDSFGDIGASAGGPDGLFDHDTRYLSQLELLINGAQPLLLHSEIKADNLSFSVDLTNP
                     DIYRDGKLWLPKDTVHIARAIYLYHGSLRERMCLTNHGPDVVNITLALAFASDFADIF
                     EVRGIRRERRGESWAQLTGASSVLLSYRGLDNQVRETSLSFEPAPSLLQSGIASYAVS
                     LAPGAKHAIFLSVAGREQLPKSTLSFFTGLLHLNRERRIDIEGSASVETSNSLLNEVL
                     HRSRADLHMLMTATPQGHYPYAGIPWYSTTFGRDGIITAMQMLWLDPNIAAGVLRRLA
                     YLQATEVDSRADAAPGKIVHEMRGGEMAALGEVPFGKYYGSVDSTPLFVMLAGGYLQR
                     TGDRGLIEQLWPAIERALDWIDGPADIDGDGFIEYARGTETGLANQGWKDSHDAVFHA
                     DGALARGPIALVEVQGYVYAAKKEAAVCARRLGKSARGAELERQAEALRQRFERSFWS
                     EEIGSYVLALDGDKAACKVRSSNAGHALFTGIADAARAQEIAGRLLLFPYYSGWGVRT
                     VAHGEARYNPMSYHNGSIWPHDNALIAAGLARYGHGAAVETIFEAIVSAALHMHDRRM
                     PELYCGFRRRPGRGPTLYPAACSPQAWAAGTPLALLAAMLGLKFDPGQHTVRLVKPVV
                     PTLAGSITVRNLTLGGASVDFVLDSSKGRPRLEVMRASGPVRVLLDA"
     gene            4627..4764
                     /locus_tag="VFR00_08690"
     CDS             4627..4764
                     /locus_tag="VFR00_08690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0159378.1"
                     /translation="MSMDTDAASEAAQLGGLTRVLPASVVFLLAHVLFVGGAIFFASM
                     N"
     gene            4849..5343
                     /locus_tag="VFR00_08695"
     CDS             4849..5343
                     /locus_tag="VFR00_08695"
                     /inference="COORDINATES: protein motif:HMM:NF027567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEU0159379.1"
                     /translation="MNAEPDQHLYQLIRDFKVAMLVSHGAGGGLHARPLAVAEISADG
                     DIVFATSIASPKVAEIAAEERVLVTFQGGSAFAAVAGRARISRDRERLAGLWSESWRV
                     FYPGGLEDPSLCLLEVTPSAAEYWDNSGIKSLKYLFKGAKAYLLGERLETDAAHAHAK
                     LDLT"
     gene            complement(5414..6685)
                     /locus_tag="VFR00_08700"
     CDS             complement(5414..6685)
                     /locus_tag="VFR00_08700"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HEU0159380.1"
                     /translation="MPARRSRREVLKLMSAASLGAAAPARLWPCPALAQGESASAAPF
                     ILMDGHVHVTNRIYWENIDPWQPTARGWDYARARAAGVNCIIENIGTYGYWNYNYSPK
                     QTLRLIETFHRFAETHADKMGVARSVPEARAIVASGRMAVFLGIESGFDHEGDPDVMA
                     AFYRLGLRSIQFATQSGFNAFADSALAATQGGQQPEHYHGINARGRELVGRMNELGIL
                     IDITHGTEAVHLQLIEASRAPVVASHDVIRAVSGVGLSDAVLKALAAKGGLVGIHGGA
                     AVVSKRYRQWLQANPAGAANAAKAVTAMVGFAPSAPRAPGDRGEYIAEMDAEFKRRWQ
                     GLDAWREDPSAALPTEAEWAEQVDYVVKTVGADHVGLGLDLVAGRSAVPATPEGYPAL
                     LAQLRRITSEANVRKIAGENWLRVLNAAKAA"
     gene            complement(6770..>7170)
                     /locus_tag="VFR00_08705"
     CDS             complement(6770..>7170)
                     /locus_tag="VFR00_08705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0159381.1"
                     /translation="HLFEVYASQAGRALGDGMGMLRDIIIADTDAEARQLWIDSATFA
                     GNAWFVPFGFRRGMLDPDTGEAPSPEAAIAKGYALVGTLDTVTRSLEALRRKLPVNWI
                     FGWTHNTLVPHAKLMRSIELFYTQVLPRVS"
BASE COUNT         1192 a         2405 c         2306 g         1267 t
ORIGIN      
        1 gtttcggaca cgcggccgcc ggcggcgccg gagccaaggc cgttcttgaa gtgcgcagcc
       61 acaaagtccc cttcattttg gaggacgcgc agatcgtcgg gcgcctaatt tatgaacggg
      121 tgacggaagt gccggaggtc ctctacggcg cctcgttgca gtccaattac cagggacaag
      181 gcctcaaatt gtccaagcac ttcaggtaag acttgaaaaa ggctggcggc cccgcgcgcg
      241 ctggcacctg cgaccccggg ggcgccttcc ttcctgcctg atcctgcctt attggagacc
      301 tgacagtgtc ttatcagcgg gggcgggcgc ggcccggtcg ccggcgaggc ggcgcgtgtg
      361 gctcgcgagg gagtgcctaa cggcggggga agccagcgtg aagcgaactt cggctttaat
      421 cggtattgtg ctggggctcg cgctggcaac ggccgcccgt gcggacagcg ccgccgattg
      481 cttcagcgaa gatctcgacc ggcgcatcga gggctgcacg gctctgatcc aacaaggtga
      541 ccagggtgcc gccgacttga gctatgccta tgccatgcgc gcgctcgcct attctctcaa
      601 gggacgctac gaaacggcca ttcgcgacta tgatgtcgca atcgccatga agcccgactt
      661 tgcagtggcg ctcaacaatc gcgcctgggc cttgttccgc tggggcaagg cctcggaggg
      721 cttgccggac gtcgaacgct cgctcgagct cagtccgacg agtgcccact cgttggacac
      781 gcgcgcgcac atcaagcagt ccctcgggga tcccaaggcg gcgctgcgcg attatgatcg
      841 cgccatgtgg tacggcggcg cgcgcatgat caaactttat cagtgcggcc tgaccgaaca
      901 aaagctctac acgggcgaaa tcgacggaac ctggcggccc gaggtgacca aggcgctcga
      961 acagtgcgtg ttggacaagg cctgcgaccc gttgcccgcc gacgagcagt gccgcgccgc
     1021 cacctcctaa cttcgcgcga acgcggaact gacgcgaggc gaacgcgccc aggcctcaga
     1081 cctgagggcc ggcggaggcg cgaaagcggg gctgcccccg cgctctgcgc ggggaacttc
     1141 acttgcttta gcacgtttag tccgggcttg gttccctaac aacgcgtgtc ccatgaaaat
     1201 cgcccagatt gcgccgccga tggagagtgt cccaccccgc ctctacggcg gtaccgaacg
     1261 catcgtctcc tacctgaccg aggaactcgt ggccctgggc cacgacgtaa cgttgttcgc
     1321 cagcggcgat tccatcacgc gcgccaagct catcgccagc tgcccgcaag ccttgcggct
     1381 caatccggcg gtgcgcgatc ccattcccta ttacatgatg atgctctcga aggtgcgccg
     1441 cgccgccgcc gaattcgaca ttttgcactt tcatatcgac cagtttcact ttccgctctt
     1501 tgaggacatg gccgcgcgcg tggtgacgac catgcacggc cggcaggacc tgcccgatct
     1561 gcaaaatttg tatgcgggct tcccctccat gcaactggtc tccatctccg aggcgcagcg
     1621 ccggccgctg accggggcgt cctttgccgc caccgtctat catggcctgc cgcgcgacct
     1681 cttgtccccg tcgctggttc cgcgcggcgg ctatctggct ttcctggggc gcatctcgcc
     1741 agaaaagcgg gtcgaccgcg ccatcgccat tgcccgcgcc gttggcgtgc ccatcaagat
     1801 tgccgccaag atcgatcgcg tcgacgagga ctacttccac gccgagatcg agcctctgct
     1861 caaccagccc ggcgtcgagt acattggcga aatcgatgac cgcaaaaagt cgcgctttct
     1921 cagcgatgcg cgcgcgctgc tgttcccgat caattggccg gagccgttcg ggttggtgat
     1981 gatcgaggcc atggcctgcg gcacgccggt gctcgccttc gaagcgggcg ccgtgcggga
     2041 aatcatcgat gagggcgtga ccggccacat cgtgcgcagt gtcgaggaag gcatctgcaa
     2101 gatcggcgcg gtgatggcgc tcgatcgcgg ccgggtgcgg cgccgcttcg aggaacgctt
     2161 cactgcgaca cgtatggcca acgactatgt taaggtctac caagccctgc ttggcgaaga
     2221 ggcccgcgcg cggcccggct gggagccagc cgaagtggcg agcgtggccg agccggtggc
     2281 ggccgttcac tgacaccacc acggggggct aagaggccag gcgcgcagaa gtggtcgttt
     2341 ctcaccccgc caaaccgagc gcccctgcga gcgcccccac cgaggcgccg ttctatattc
     2401 ctgccaccaa ctccccgtcg cgcccgcgcc gaacgctgaa gcacgacgac accttcgcgg
     2461 tgttcgacag ctttggcgat atcggcgcca gcgccggcgg tcccgacggc ctattcgatc
     2521 acgacacgcg ctacctgtcg cagctcgagc tcttgatcaa tggtgcccag ccgctgctgc
     2581 tccattccga gatcaaggct gacaacctca gtttttccgt cgacctcacc aatcccgata
     2641 tttatcgcga cggcaagctg tggctgccca aagatacggt ccacatcgcg cgtgcgattt
     2701 atctctatca tggctcgctg cgcgagcgca tgtgcctcac caatcacggc cccgatgttg
     2761 tcaacatcac cctggcgctg gccttcgcca gcgactttgc cgatatcttc gaagtcagag
     2821 gcattcgcag ggaacggcgc ggcgaaagct gggcccagct caccggtgcc agcagcgtct
     2881 tgctctccta tcgcggtctc gacaaccagg tgcgcgaaac cagcctgagc ttcgagccag
     2941 cgccaagcct gctgcaaagc ggcatcgcca gctacgcggt cagcctggcg cccggtgcca
     3001 agcacgccat cttcctcagc gtggccggca gagagcagtt gccgaaatcg acgctgtcgt
     3061 tcttcacggg gttgttgcac ctcaaccgcg aacgccggat cgacatcgag ggctcggcga
     3121 gcgtggagac cagcaatagc ctgctcaacg aggtgctgca ccgctcgcgc gcagacctgc
     3181 atatgttgat gacggcaacg ccgcaggggc attaccccta cgccggcatc ccctggtatt
     3241 cgacgacctt cggtcgcgac ggcatcatta cggcgatgca gatgctgtgg ctcgacccta
     3301 atatcgccgc gggcgtgctg cgccggcttg cctatctgca agcgaccgag gtcgacagcc
     3361 gcgccgacgc cgcgccggga aaaatcgtgc acgagatgcg cggtggcgaa atggcggcgc
     3421 tcggcgaggt tccgttcggc aagtactacg gcagcgtgga ctcaacccca ttgttcgtca
     3481 tgcttgccgg cggttacctg cagcgcaccg gcgatcgcgg gctcatcgag cagctttggc
     3541 cggcgatcga gcgcgcactc gactggatcg acggtcccgc cgacatcgat ggcgatggct
     3601 tcatcgagta cgcgcgcggc acggagacgg ggcttgccaa tcagggttgg aaggattccc
     3661 acgacgccgt ttttcacgcc gacggcgcgc tcgcgcgcgg cccgatcgcc ttggtggagg
     3721 tgcagggtta tgtctatgcc gccaaaaaag aggcggcggt gtgtgcccgc agactgggga
     3781 aatcggcgcg cggggccgag ctcgagcggc aagcagaggc cttgcgccag cgcttcgagc
     3841 gcagcttctg gtcggaagag atcggcagct acgtgctggc gctcgatggc gacaaggcgg
     3901 cctgcaaggt acgtagtagc aatgccgggc atgccctgtt taccggcatc gccgatgccg
     3961 cgcgcgcgca agagattgcc ggccggctgc tgctcttccc ctactattcg gggtggggcg
     4021 tgcgcaccgt tgcgcatggc gaggcgcgct acaatcccat gtcctaccac aatggctcga
     4081 tctggccgca cgataacgcg ctgatcgccg ccggcctcgc gcgctatggc cacggcgccg
     4141 cggtggagac gatctttgag gcgatcgtga gcgccgcgct gcatatgcac gatcgacgca
     4201 tgccggaact ctactgcggc ttccgccgcc gccccggccg cggtccgacg ctctatccgg
     4261 ccgcctgctc accgcaggcc tgggccgcgg gcacaccgct tgcgctgttg gccgcgatgc
     4321 tcggcttgaa gttcgatcct ggccagcaca ccgtgcgcct cgtcaagccg gtggtgccga
     4381 cgctggccgg cagcatcacc gttcgcaatc tgacgctcgg gggagccagc gtcgatttcg
     4441 tgctggacag cagcaaaggc aggccgcggc tcgaggtcat gcgcgcctcg gggccagtgc
     4501 gcgtgctgct cgacgcctga ggcgccgcac cgcgcggagg tgtcgggatc accgcggcgc
     4561 ctcgagcgcc ggaaccacgc cgcccttcct tgagtttagt gggtcgatca ccccgaaggg
     4621 agccagatga gcatggacac cgatgcggca agcgaggccg cccaattggg cggattgacc
     4681 cgggtcttac cggcctccgt ggtatttctt ttggcgcacg tgctgttcgt cggcggcgcc
     4741 atcttctttg cctccatgaa ttaacccggc gctcggctgg cgccgggtcg ccgagcgaag
     4801 tggcattttt gttgcagctt taaaccgctt gagctttggg cctattgcgt gaacgccgaa
     4861 ccggaccagc acctttatca gctgatcaga gatttcaagg tcgccatgct ggtcagccac
     4921 ggcgcgggtg ggggtctgca cgcgcgtccc ctcgccgtcg ccgagatttc cgccgacggc
     4981 gacatcgtct tcgccaccag cattgcctca cccaaggtgg ccgaaatcgc cgccgaggag
     5041 cgcgtgctcg tgacgtttca gggcggctcg gcgttcgccg ccgtggccgg cagggcgcgg
     5101 attagccgcg atcgcgagcg cctcgccggg ctgtggtcgg aaagctggcg cgtgttctat
     5161 cccggcggcc tcgaggatcc ctcgctgtgc ctgctcgagg tcacccccag cgcggcggag
     5221 tactgggata acagcggcat caagagcctc aaatatctgt tcaagggcgc caaggcctac
     5281 ctgttgggcg agcgcttgga gaccgacgcg gcccatgccc acgccaagct cgatctgacc
     5341 taggttgagg gcctttgctt gcagtatggc ggcgccgtct ggtggggcac aaccgtcggg
     5401 ccctgcggcg cgcttaggcc gccttcgcgg cgttgagcac ccgcaaccag ttctcgccgg
     5461 caattttgcg cacattcgcc tcgctcgtga tccgacgcag ctgggccagc aacgccggat
     5521 agccctccgg cgtcgccggc accgcgctgc ggccggccac cagatcgagg cccaagccaa
     5581 cgtgatctgc gccaaccgtt ttaaccacgt aatcgacctg ttcggcccac tccgcctcgg
     5641 tcggcaaggc ggcgcttgga tcctcgcgcc aggcgtcgag cccctgccag cgccgtttga
     5701 attccgcatc catttcggcg atgtattcgc cgcggtctcc gggcgcgcgc ggcgcggagg
     5761 gggcaaagcc caccatggcg gtcacggctt tggccgcgtt ggccgcgccc gccggattgg
     5821 cctgcagcca ctgccgatac cgtttggaga ccacggccgc gccgccgtgg ataccgacga
     5881 ggccaccctt tgccgccagc gccttgagca ccgcatcgga caaaccgacc ccgctgacgg
     5941 cgcggatgac atcatggctg gcgaccacag gcgcccggct ggcctcgatc agctgcaagt
     6001 gcacggcctc ggtgccatgc gtgatgtcga tcaggatgcc gagttcgttc atgcgcccca
     6061 ccagctcgcg tccacgcgcg ttgatgccat ggtaatgctc gggctgttgg ccgccctgcg
     6121 tggcggcgag cgcgctgtcg gcgaaggcat taaacccgct ctgggttgcg aattgaatgc
     6181 tgcgcaagcc aagccgataa aaggctgcca tcacgtccgg atcgccctcg tgatcgaagc
     6241 cggattcgat gccgagaaaa accgccatcc gcccgctggc gacgatagcg cgtgcctccg
     6301 gcacgctgcg tgccacgccc atcttgtcgg cgtgcgtctc ggcgaagcga tggaaggtct
     6361 cgatcagccg cagcgtctgc ttgggggagt agttgtagtt ccagtagccg taggtgccga
     6421 tgttttcgat gatgcaattg acccccgctg cccgcgcccg cgcatagtcc cagccccggg
     6481 ccgtcggctg ccacggatct atgttctccc agtaaatgcg gttggtgacg tgcacgtgcc
     6541 catccatcaa aatgaacggc gcggccgatg cgctctcgcc ctgcgccagc gccgggcagg
     6601 gccataggcg ggcgggcgcg gccgcgccga ggctagcggc gctcatcaat ttcagcacct
     6661 cgcggcgaga tcgtcgggcg ggcatgggct cctccgtttg agcgtgcttc tgtgccgccc
     6721 gctaggcccc tcgcgccacc ggcgccgcgc cccggcacgg gcaaaaggcc taactcacgc
     6781 gcggcaacac ttgcgtatag aacagctcga tcgagcgcat cagcttggca tgcggcacca
     6841 gggtgttgtg ggtccagcca aagatccaat tgaccggtag cttgcggcgg agcgcctcca
     6901 ggctgcgcgt caccgtgtcg agcgtaccga ccagcgcgta gcccttggcg atcgctgcct
     6961 cggggctcgg cgcctcaccc gtgtcggggt cgagcatgcc tcggcgaaag ccgaagggca
     7021 cgaaccaggc gttgcccgcg aaggtggccg aatcgatcca gagctggcgg gcctccgcat
     7081 ccgtatcggc gatgatgatg tcgcgcagca tgcccatgcc atcgccgagc gcgcggcctg
     7141 cttgggaggc atagacttcg aatagatgct
//
LOCUS       DASTMT010000373        14347 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_5028294, whole genome shotgun sequence.
ACCESSION   DASTMT010000373 DASTMT010000000
VERSION     DASTMT010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 14347)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14347)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14347
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5028294"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1496
                     /locus_tag="VFR00_08710"
     CDS             <1..1496
                     /locus_tag="VFR00_08710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019402106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEU0159382.1"
                     /translation="TTKRHPALIHAEAAADGEGRLTAFRMRADFDTGAYASWGPTVAN
                     RVPVHGMGPYRVANAEIATRAVFTNATPAGAFRGFGVPQAAIAHETLLDDLAEKASLD
                     RWTIRYRNALQAGDRTASGQRLAHSVGLRQCLCALKPDWDAALARAAAHNAAQQRWRR
                     GVGLAAMWYGCGNTSLPNPSSMRITLARDGRLTFCNGAVDIGQGSSTVLLQIAAASLG
                     LPPEAFQMLVGDTALTPDAGKTSASRQTFVSGNAVRRAATALRGKILALANAGPDARL
                     ELVGSRLGIVDGEASRSIELSALPPLAAIAPAHGAGALTAAALEAEALGEVVLEGSGS
                     WDPPTTPLDANGQGIPYATYGFAAQLAELEVDISLGTVKVLAMVAAHDVGRAINPTLV
                     EGQIHGGIAQGLGLALMEEFAPGRSENLHDYLIPTVGDMPSVKIYLIEDEEPEGPFGA
                     KGVGEPALIATAPAILGAIRHATGVRLTRVPVLPHRLWQALNAEKTA"
     gene            1493..3013
                     /locus_tag="VFR00_08715"
     CDS             1493..3013
                     /locus_tag="VFR00_08715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015927945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-hydroxynicotinate reductase"
                     /protein_id="HEU0159383.1"
                     /translation="MSGDRLSLAARANKLGEAEDGGIVRCDACPVLCRIREGRLGACA
                     RYGNVAGKLVRTDPVVLMAKTVDAKGKLVAFASDDWEGTPLDPARTFLTGIGAGTTYP
                     DYKPAPFIVAAEHDGVDTVTVVTEGIFSYCGVKLKIDTDRHLGPEAAPVRVKGEQVGH
                     VTTAEYGSQMLSIGGVRHLTGGSKKEGNVTCDTLLELCSGEPVSISIDGGRALVVAAG
                     KPPIVDGREEVRMRVGCGSATVGIFARQWFGHLDEVIVVDDHITGVLSEHQSGRVLKM
                     PPAGIRVRGRKSTPGRYFKVAEPGLGWGGTDITDPLSIIQKIDPAVAWPGLRLLITST
                     TGEDALYCVLDEDLRPVPAPLPAPVRAVIDRIGENCEPALCSVAFMAGAGGSLRAGVT
                     ENPILLTRSVASGETRVTMGGAPVTLWPGGGITIMVDVTRMPKRAFGSVPTPAIVAPI
                     EFTLPRAFYERLGGHAADIVSIEEVIRSLAPDARIDAWPQDNPWPFLSGKPDRDQP"
     gene            3028..3537
                     /locus_tag="VFR00_08720"
     CDS             3028..3537
                     /locus_tag="VFR00_08720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019862484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HEU0159384.1"
                     /translation="MAPAHAKVHRRPAAKPRPAAGSYVLEQQVGFLLRCAHQRASETF
                     NAVMGRFGVTPRQFAALAKLDDLGSVSQNQLGRLTAMDPATISGVIARLAARGYVAQA
                     ADPRDGRLLALALTAAGAAAVGAMKAVAAEVSQLTLAALSEQEAAAFLEALCKIGQMP
                     CLPRPTSRP"
     gene            3486..4370
                     /locus_tag="VFR00_08725"
     CDS             3486..4370
                     /locus_tag="VFR00_08725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012331860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0280 family protein"
                     /protein_id="HEU0159385.1"
                     /translation="MQDRSDAMFAPAHVQALTGGRLHLSHGPIDIVLKAWGGEKARRA
                     AYACAGQRFASILPELCDELAQLRTPVSETPVLEGPVARRMLEACLPFHGTFLTPMAA
                     VAGAVADELLAHMLEAACLQRVFVNNGGDIAIFLAPGQALEVGIAGEFSRGNVPALNG
                     YLRLEAGMGVGGIATSGARGRSFSLGIADSVTALGRTAAAADAAATLIANAVDVDSSA
                     VSRRPARALDPDSDLGDRLVTVAVGALSAAQIASALEAGLARALSYRRQGLISDAALM
                     LAGETRLLPAAQTNGDGR"
     gene            complement(4387..5280)
                     /locus_tag="VFR00_08730"
     CDS             complement(4387..5280)
                     /locus_tag="VFR00_08730"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU0159386.1"
                     /translation="MQIGVAGVGKMGSAIALRLLEVGHAVTVWNRSAEKVEPLARAGA
                     GRAASAAALAGEVEVIITILSNAEAQLGVYEGAAGLLAGGIAGKLVIDMSTVQPEHER
                     ALAAKVTAKGAHFVECPVGGTTGPARQGKLLGLAGGTGPDVERARPILKELCRRLEHL
                     GPVGAGSSMKLAINLPLLVAYQALGEAYLLCRHVGLSPQALMELFADTSGAPNILKVR
                     GPAIAEALAGGPGMAAAFDVDSIRKDLNTMLAEAKGLGAELPLAERTLAIFDEAAGQG
                     WGKRDGSALPSYWSRRRDGKS"
     gene            complement(5385..6455)
                     /locus_tag="VFR00_08735"
     CDS             complement(5385..6455)
                     /locus_tag="VFR00_08735"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018643059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEU0159387.1"
                     /translation="MQLGFFTMPIHPPAKDWRQSLREDREAFVLADELGFVEAYAGEH
                     ATDRCENITSSAMFLATLVDRVKRMRLGTGTLNLPNAHPVAVASQIAMLDHLLEGRFN
                     LGISPGGLLSDAEAFGNLDANRNEMFLELINQVLAVWAGEAPYDLAGKYWNISTARTL
                     MPDIGQGYIGKPLQQPHPPIVVTAVAPFSKGVTEAAARGWDPISANFLMPVWVKSHWP
                     RYVEGCERAGRSPDPANWRVAKSIFVADDDRTARDYVTAPNSPYRQYYDSLATKLRKG
                     GRLELFKRSRDMPDAEVTLDFICEELVLWGSVDRVVDEIQKFRAEVGPFGTLLYAGKD
                     WLDYDLGRRSMVLMAERVLPKL"
     gene            6604..7494
                     /locus_tag="VFR00_08740"
     CDS             6604..7494
                     /locus_tag="VFR00_08740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007199049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEU0159388.1"
                     /translation="MREPMTPRERAPYSAIVDRQPLALPGGARIIVWTIVNLEVWDIS
                     RPMARQVLPPPTGQVLLPDNPNWSWHEYGMRVGVWRFFALFKHLGLRPSLSINARVCQ
                     DYQRVASEARDAGWEPMGHAFEQMPIHRIEDQRGMIAQSLDTLEAFWGERPIGWLGPG
                     LTQTLETPELLAEAGVRYIGDWVYDDEPTQIRTANGPLVTLPYTAELNDIPVMVLQHH
                     EGAYWLRKCIDAFDRLYQEGATRPRVMAIAIHPYISGQPFRIKYLEQVYEHISQFAGV
                     LHWNGAEIYRWYQRTVPLQG"
     gene            7588..9039
                     /locus_tag="VFR00_08745"
     CDS             7588..9039
                     /locus_tag="VFR00_08745"
                     /inference="COORDINATES: protein motif:HMM:NF012862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HEU0159389.1"
                     /translation="MARFIALLFVLIALWPPTPAQADKRVALVIGNSAYQNTSELRNP
                     SNDASDIASALTRLGFQVTDGRDLDKRAMERTIRQFGLALEGADIALFFYAGHGIQVG
                     GQNHLIPVDARLASEGDVDFETLPLSLVLKQMEREAKTSLVLLDACRDNPLARNLARS
                     MGTRAITIGTGLAEVKTGIGTLIGFSTQPGNVAADGVGRNSPYAGALLKHIEDSAKDV
                     SGILVDVRNDVLKATNGTQVPWEHTSLTGQVYFRLEAPPPGPAPAAPSGRNYDKEMEI
                     ALWDAVKDSKTPAILKTYLDRFPKGTFAGLARVLMEEANKAGRSGAAPADPGSSSGAA
                     SPSAGTSAPTAVSKDPRALARALQAELKRVGCDPGAIDGNWGKKAEDALAEFARQSKT
                     TLTREASQEALKAVAAQPTRVCRLVCGGDEMERDGKCVAKAAPARRRPQVVETAPKRK
                     KESTSGQMCWSNDRRNMAVVPCGDAASSGQRAY"
     gene            complement(9055..10182)
                     /locus_tag="VFR00_08750"
     CDS             complement(9055..10182)
                     /locus_tag="VFR00_08750"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEU0159390.1"
                     /translation="MHPLKGVKVVEIANNIAGPYAGYVLAMLGADVLKVERPEGDDAR
                     GWGPPFWLDTSATFHALNLNKRGITLDLKDGTALAWLKGYIGEADVLLHNLRPGVMEA
                     LGLGSAAMTALNPRLVYCNLSAFGPTGPMQKNPGYEPMVQAFAGLFSINGYPDRPGVR
                     IGTSVLDLGTGVWTALGCIAGLLERARSGKGAIVDTSLFETALGLLTVHFARYQASGQ
                     LPERHPTGSLGVVIFEGFETADGQVIVAAANDRLFAKLAGELGHPEWVNDPRYKTNPQ
                     RIANKASLIGAVAEILRQRPSAEWVERLEKIGVPCAPINDMSIMKDHPQTAALDILQP
                     VPDLALNLIGLPVSFDGERPRILTRAPTLGQHNAMLPPAKA"
     gene            complement(10223..11038)
                     /locus_tag="VFR00_08755"
     CDS             complement(10223..11038)
                     /locus_tag="VFR00_08755"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002713364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEU0159391.1"
                     /translation="MSEVYQDILYEVRERVATITLNRPDRLNAYTPNMGDELRKALAR
                     ADQDEAVRAIVLTGAGRGFCAGADMARLSAAVAGKTSLKVSETAPSVGLAANFAQRNS
                     YILAISKPIIAGINGAVAGIGLVLTLYCDVRYMAAGAKLTTAFARRGLIAEHGISWML
                     PRLIGPMHAMDLLLSARVVAAEEAERLGLVRVLPAEGFAAAVQQRAAELANLSSPRSV
                     RIIKRQVYEGLFQSLAEATAIANREQEVCRETEDFREGVAHYLEKRPPNFSGR"
     gene            complement(11125..12024)
                     /locus_tag="VFR00_08760"
     CDS             complement(11125..12024)
                     /locus_tag="VFR00_08760"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0159392.1"
                     /translation="MATISHPGGGAAIDQAPPRPSFWQLLWRFCVQNPLGAGGGVVVI
                     VMMIMAIFAEEITRFDPTANNFGEMLTAPNLDYWLGTDQFGRDLYTRIVYGARTALLV
                     GFVSAFAGASIGLILGVASAYFGGLFDLIFQRVMDIVMAFPLVILALAVVSIFGTGAQ
                     NVILAITIPFIPRCARVVRSSALAVRETPFIDAARACGFSHTRIVLRHMVPNVMAPYI
                     IMMTAAVGQAILIEASLSFLGLGVQEPTPAWGLMLRGGAQEYAESAPWVAIFPGVAIS
                     LAVFGFNLFGDALRDWLDPKMKV"
     gene            complement(12037..13011)
                     /locus_tag="VFR00_08765"
     CDS             complement(12037..13011)
                     /locus_tag="VFR00_08765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012564651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0159393.1"
                     /translation="MYFYVFKRLTLMIPTLLGAAALVFFLMRLIPGDICTLRLATGGG
                     SINPNVVKACRLELGMDQPLLMQFWFFIKGLITFDFGRSMWTGRPIVEEVGLRFQLSL
                     QVAIMATLTAIALAIPLGTISAVKQNTWIDYLVRAFSIAGVAMPSFWLGILIILAMLI
                     TSKALFGSPWMPPIKYVPIWVDPVYNLSMLIWPALATGYRYSAVATRMTRSAMLEVLR
                     EDYVRTARAKGVFRRLIVRRHALRNALLPVITIIGIEFAFLMGGLVVTEQVFNLNGLG
                     KLFVESVSNQDYTMTQALVMLVVAIFAFTNLVVDVIYAWLDPRIRYGR"
     gene            complement(13055..>14347)
                     /locus_tag="VFR00_08770"
     CDS             complement(13055..>14347)
                     /locus_tag="VFR00_08770"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0159394.1"
                     /translation="IRKGVKFHDGSALTAKDVNASFQHIIFPPDGVQSPRKEHFHMVE
                     SVSAPDDETFVMKLKYPSGAFLPALASPYHFIYAKAILDKDPHWFEQNVMGSGPFVFE
                     AREAGAAVRGKRNPDYYHAGLPYLDGFEAIFAKTQSLRVQAIRGDQAAIEFRGLPPKS
                     RDDLVAALGKDITVQESDWNCLLIVTPNSRKKPFDDVRVRKALTLALDRWSGSRDLSK
                     IAVVKSVGGIVFPNHPLAASKEELAALAGYSTDIKAAREEAKRLLKEAGAADLKFTLA
                     NRAVDQPYTIVGTWLIDQWKQVGIGVEQKMEPTAPFYATLENGAFDVALEFNCQSVVN
                     PLGDTSKFLPNAGDQYGGFKDPEMAKLYEAMNRTGDVAEQRRLMRAYEKRAIDEEVHN
                     FITFWWYRIIPHRSYLKGWKISPSHYLNQDLSMVWLDK"
BASE COUNT         2378 a         4680 c         4933 g         2356 t
ORIGIN      
        1 cgaccaccaa gcgtcacccc gcgctgatcc acgccgaggc cgcggccgat ggcgagggcc
       61 ggctcaccgc ctttcgcatg cgggccgact tcgataccgg cgcctatgcc tcctgggggc
      121 cgacggttgc caaccgcgtg cccgtgcacg gcatggggcc ctatcgggtc gccaacgccg
      181 agattgcgac gcgggcggtg ttcaccaacg ccacgcctgc cggtgccttc cgtggcttcg
      241 gcgtgccgca ggccgccatt gcgcacgaga ccttgctcga cgaccttgcc gaaaaggcgt
      301 cgctcgaccg ctggaccata cgctatcgca atgccctgca agccggcgat cggacggcct
      361 cgggccagcg ccttgcccat tcggtcgggc tgcggcaatg cctctgcgcg ctgaagcccg
      421 attgggacgc agcgctggcc cgcgcggccg cgcacaacgc cgcccagcag cgctggcggc
      481 gcggcgtcgg cctcgccgcc atgtggtatg gctgcggcaa cacttcgctg cccaatccct
      541 ccagcatgcg catcacgctg gcgcgcgacg gccggctgac gttttgcaac ggcgccgtcg
      601 atatcggcca gggctcctcg accgtgttgt tgcaaatcgc cgccgccagc ttggggctgc
      661 cgccggaggc cttccagatg ctggtcggcg atacggcgct gacgccggat gccggcaaaa
      721 cctcggcctc gcggcaaacc ttcgtctcgg gcaatgcggt ccggcgcgcg gccaccgcgc
      781 tgcggggcaa aatcctggcc cttgccaatg ccggccccga cgccaggttg gagctggtgg
      841 gcagccgcct ggggatcgtg gacggggaag cctcgcgcag catcgagctc tcggcgctgc
      901 cgccgctcgc cgccatcgcc cccgcgcacg gggcgggcgc gctcaccgcc gccgcgctcg
      961 aggccgaggc gctgggcgag gtggtgctgg agggcagcgg cagctgggac ccgccgacca
     1021 cgccgctcga tgccaacggc cagggcattc cctatgcgac ctatggtttt gccgcgcagc
     1081 tggccgagct ggaggtcgac atcagcttgg gcacggtcaa ggttttggcc atggtggcgg
     1141 cccacgacgt cggccgcgcc atcaatccca cgctggtcga gggccagatc cacggcggca
     1201 ttgcccaggg cctcgggctg gcgctgatgg aggaattcgc gccgggccgc agcgaaaacc
     1261 tgcacgacta tctcattccc acggtgggcg acatgccaag cgtgaagatc tacctgatcg
     1321 aggacgagga gcccgaggga ccgtttggcg ccaagggggt cggtgaaccc gccctgattg
     1381 ccaccgcgcc cgccattctc ggcgccatcc ggcacgccac cggcgtgcgg ctgacgcgcg
     1441 tgccggtgtt gccgcatcgc ctgtggcagg cgctaaacgc ggagaaaaca gcatgagcgg
     1501 cgatcggctc tcgctggcgg cgcgcgccaa caagcttggt gaagcggagg acggcggcat
     1561 cgtgcgctgc gatgcctgcc ccgtcttgtg ccgcatccgc gagggacgct tgggcgcctg
     1621 cgcccgctac ggcaacgtgg cgggcaagct ggtgcgcacc gaccccgtcg tgctgatggc
     1681 aaaaaccgtc gatgccaagg gcaagctggt ggcctttgcc agcgacgact gggaaggcac
     1741 gccgctcgat cctgcgcgca ccttcctgac cggcatcggt gcgggcacca cctatcccga
     1801 ctacaagccg gcgcccttca tcgtggcggc cgagcacgac ggcgtcgata cggtcaccgt
     1861 cgtcaccgag ggcatcttca gctactgcgg cgtcaagctg aagatcgaca ccgatcggca
     1921 tctgggaccc gaggcggccc cggtgcgggt caagggcgag caggtcgggc acgtgacgac
     1981 ggccgagtat ggctcgcaga tgctctcgat cggcggggtg cggcacttga ccggcggctc
     2041 caagaaggaa ggcaacgtca cctgcgacac cctgctcgag ctgtgctcgg gcgagccggt
     2101 gtcgatcagc atcgatggtg gccgcgccct cgtggtggcg gcgggcaagc cgcccatcgt
     2161 cgatggcaga gaagaggtca ggatgcgcgt tggctgcggc tcggccacgg ttggcatctt
     2221 tgcgcgccag tggttcggcc acctcgacga ggtgatcgtg gtggacgacc acatcaccgg
     2281 cgtcctgagc gagcatcagt cggggcgggt gctgaagatg ccgccggcgg gcattcgcgt
     2341 gcgcggccgc aagtcgacgc ctgggcgcta cttcaaggtc gccgaacccg ggcttggctg
     2401 gggcggcacg gacatcaccg atcccctctc gatcatccag aaaatcgatc cagccgttgc
     2461 ctggcccggg ctcaggctgc tgatcacgtc gacgacgggc gaagatgcgc tttattgcgt
     2521 gctcgacgag gacttgcgcc ccgtgccggc gccgctgccg gccccggtcc gcgcggtcat
     2581 cgatcgcatt ggcgagaact gtgaaccggc gctgtgcagc gtcgccttca tggctggcgc
     2641 cggcggctcg ctgcgcgcgg gcgtcaccga aaacccgatc ctgctgacgc gctcggtggc
     2701 gagcggcgag acccgggtga ccatgggagg cgcgccggtc acgctgtggc ccggtggcgg
     2761 cattaccatt atggtcgacg tgacgcggat gccgaagcgg gccttcggtt ccgtgccgac
     2821 gccggcgatc gtcgcgccga tcgaatttac cctgccgcgc gcgttctacg agcgcctcgg
     2881 cggccacgcc gccgatatcg tgtccatcga ggaggtcatc cgcagcctgg cgcccgacgc
     2941 gcgcatcgat gcctggccgc aggacaaccc gtggccgttc ctgtccggaa agccggaccg
     3001 cgatcagccc tagaagagga gcccgccatg gcccccgccc acgccaaggt gcaccgccgg
     3061 ccggcggcaa agccgcgccc ggccgccgga tcttacgtgt tggagcagca ggtcgggttt
     3121 ttgttgcgct gcgcgcatca gcgcgccagc gagacgttca acgccgtcat gggtcgcttc
     3181 ggcgtgacgc cccggcagtt tgccgcgctg gccaagctcg acgatctggg ctcggtgtcc
     3241 cagaaccagc tcggccgtct caccgccatg gaccccgcca ccatttcggg cgtgatcgcc
     3301 cggctcgctg cccgcggcta cgttgcccag gccgccgatc cccgcgatgg ccggctgttg
     3361 gcgctggcgc tgaccgcggc gggcgcggcg gccgtcggcg ccatgaaggc ggtggccgcc
     3421 gaggtctcgc agctgacgct ggcggcgctc agcgaacagg aagcggcggc cttcctcgag
     3481 gccttgtgca agatcggtca gatgccatgt ttgccccggc ccacgtccag gccctgacgg
     3541 gtgggcggct tcacctctcg cacgggccga tcgatatcgt gctcaaggcc tggggcgggg
     3601 agaaagcgcg gcgggcagcc tacgcctgcg ccgggcagcg ctttgccagc attttgcccg
     3661 agctctgcga cgagctcgcc caattgcgga cgcccgtgag cgagaccccg gtgctcgagg
     3721 ggcccgtggc gcgccgcatg ctcgaggcct gcctgccgtt ccacggcacg ttcctgacgc
     3781 cgatggccgc ggtggcaggc gcggtcgccg atgagctcct ggctcacatg ctcgaggcgg
     3841 catgcctgca acgcgtcttt gtcaacaatg gcggcgacat tgccattttt ctggcaccgg
     3901 ggcaggcgct cgaggtcggc atcgccggcg aattctcgcg cggcaacgtg ccggccctga
     3961 acggctacct gcgcctcgag gccggcatgg gcgtgggcgg cattgccacc tcgggtgcac
     4021 gcggccgctc gttctcgttg ggaatcgccg acagcgtcac cgcgctgggt aggaccgcgg
     4081 ccgccgccga cgcggccgcc acgctgatcg ccaacgccgt cgatgtggac tcgtcggccg
     4141 tcagccggcg tccggcccgg gcgctcgatc ccgatagcga tctgggcgac cggctggtga
     4201 cggttgcggt tggcgcgctc agcgccgcgc agatcgcaag tgcactggag gccgggctgg
     4261 cgcgggcctt gagctaccgg cgccagggct tgatcagcga tgccgccttg atgctggcgg
     4321 gcgagacgcg cctgttgccc gccgctcaga ctaacggcga cggcaggtga cggcgagcgc
     4381 cctggctcag cttttgccgt cgcggcgccg cgaccaatag gacggcagcg ccgagccgtc
     4441 gcgtttgccc cagccttggc cggcggcctc gtcgaagatg gcaagcgttc gctcggccag
     4501 cggcagctcg gcgccgagcc ccttggcttc ggccagcatg gtgttgaggt ccttgcggat
     4561 ggaatcgaca tcgaaggccg ccgccatacc cggcccgccg gcgagcgcct cggcgatggc
     4621 ggggccgcgc accttcaaga tgttgggggc gcctgaggtg tcggcgaaca gctccatcag
     4681 cgcttgcggg ctcagcccca cgtggcggca caacaggtag gcctcgccca aggcctgata
     4741 ggcgacgagc agcggtagat tgatggcaag cttcatgctg gagccggcgc cgacgggccc
     4801 caaatgctcg agccgccggc acagctcctt gagaatgggc cgggcgcgtt cgacgtcggg
     4861 ccccgttccg cccgccaggc ccagcaactt gccctgccgc gccggccccg tggtgccgcc
     4921 caccgggcat tcgacgaagt gggcaccctt tgccgtcacc ttggcggcga gcgcgcgctc
     4981 gtgctcgggc tgcacggtgc tcatgtcgat cacgagcttg ccggcgatgc cgcccgccag
     5041 caggcccgcc gcaccctcgt agacgcccag ctgcgcctcg gcgttgctga ggatggtgat
     5101 gatcacctcc acttcaccgg cgagcgccgc ggcgctggcg gcacggccgg cgccggcgcg
     5161 cgcgaggggt tcgacctttt ccgcgctgcg gttccacacc gtcaccgcgt ggccaacctc
     5221 gagcagccgc agcgcgatcg cgctccccat tttgccgacg ccggcaacgc caatctgcat
     5281 cgcggttgtc cttcttaatt tggccagggc atgcgcgggc acgtccgtgg cgctccccga
     5341 cacgcagcaa cggcccctgt cgattgcgac aggccggccc cggctcagag tttgggcagc
     5401 acgcgctcgg ccatcagcac catggagcgg cgccccaagt cgtagtcgag ccaatccttg
     5461 cccgcataga gcagggtgcc gaacgggccg acctcggcgc ggaatttctg gatctcgtcg
     5521 acgacccggt cgaccgatcc ccacagcacc agctcctcgc aaatgaaatc gagcgtgacc
     5581 tcggcatcgg gcatgtcgcg actcctcttg aagagctcga ggcgcccacc cttgcgcagt
     5641 ttggtggcga gcgagtcgta atattggcgg tagggactat tgggcgccgt cacgtagtcg
     5701 cgcgccgttc tgtcgtcatc ggcgacaaag atgcttttgg ccacgcgcca attggcggga
     5761 tcggggctgc ggccggcgcg ctcacagccc tcgacatagc gtggccagtg gcttttgacc
     5821 cagaccggca tcaaaaagtt ggccgagatc gggtcccagc cgcgcgcggc ggcctccgtc
     5881 acccccttgg agaagggtgc cacggccgtg acgacgatgg gcgggtgcgg ctgctgcaag
     5941 ggtttgccga tgtagccttg gccgatgtcg ggcatcagcg tgcgcgccgt cgagatattc
     6001 caatatttgc ccgccaaatc gtaaggggct tcgcccgccc aaacggccaa cacctgattg
     6061 atcagctcaa ggaacatctc gttgcgattg gcatcgaggt tgccgaaggc ttccgcatcc
     6121 gacaacaggc cgcccggact gatgccgaga ttgaagcgtc cctccagcag gtgatcgagc
     6181 atggcgatct gcgaggcgac ggcgaccggg tgcgcgttgg gtagattgag cgtgcccgtg
     6241 ccgagccgca tgcgcttgac gcgatcgacc agcgtcgcca ggaacatggc cgaggaggtg
     6301 atgttttcgc agcgatcggt ggcgtgctcg ccggcatagg cctccacgaa gccgagctcg
     6361 tcggcaagca cgaaggcctc gcgatcctcc cgcagcgatt ggcgccaatc cttcgccggc
     6421 gggtggatgg gcatggtgaa aaagccgagt tgcatgctga gacctttgtg gggcggctgg
     6481 gaaacctgga cgtcgcagtc ctatcccgtc ggcggcgcgg gcaaaaatca tatgttcttc
     6541 acgccatgat cgtgatttgt aatagagggg agaggttgag cgcgacagtg aggtggaagc
     6601 tttatgcgcg agcccatgac cccgcgggag cgggcaccct attcggccat cgtcgatcgc
     6661 cagccgctgg cgttgccggg cggcgcccgc atcatcgtct ggaccattgt caacctcgag
     6721 gtctgggaca tctcccggcc catggcgcgc caggttttgc cgccgccgac gggtcaggtg
     6781 ctgctgccgg acaacccgaa ctggagctgg catgaatacg gcatgcgcgt cggcgtttgg
     6841 cgcttctttg cgctcttcaa gcacttgggg ctgcgtccaa gcctgtcgat caacgcccgc
     6901 gtctgccagg actatcaacg cgtcgcgagc gaggcgcggg atgccggttg ggaaccgatg
     6961 gggcatgcgt tcgagcagat gccgatccac agaatcgagg accagcgggg catgatcgcc
     7021 caatcgctcg acacgctgga ggccttctgg ggcgagcgtc cgatcggctg gctgggccct
     7081 ggtctgacgc agacgctcga aacgcctgag ctcctggccg aggcgggcgt gcgctacatc
     7141 ggcgattggg tctatgacga cgagccgacc cagatccgca ccgccaacgg tcccctggtg
     7201 acgctgccct atacggcgga gctcaacgac atcccggtca tggtgctgca gcaccacgag
     7261 ggcgcctatt ggctcagaaa atgcatcgat gctttcgatc ggctctacca ggagggcgcc
     7321 acccggccgc gcgtgatggc gatcgccatt catccctaca tttctgggca accgtttcgc
     7381 atcaaatatt tagagcaggt ttatgagcac atcagtcaat tcgcaggtgt actgcactgg
     7441 aacggcgcgg agatctatcg ctggtaccag cgcacggtgc cgctgcaggg ctgagcggcg
     7501 acgcactttg gtcattgtgc tcacctaaga tcggctcaat tgctgtgcgc gggaaatgac
     7561 ctgaactgtt gtgagcgggg aacaaccatg gcgcgtttca tcgccctcct ctttgtcctc
     7621 atcgcgctgt ggccgccgac gccggcgcag gccgacaaac gcgtggcgtt ggtgatcggc
     7681 aactcggcct accaaaacac ctccgagttg cgcaatccca gcaacgatgc ctccgatatc
     7741 gcctcggcgc tgacgcgcct gggctttcag gtgaccgacg gacgcgacct cgacaagcgc
     7801 gccatggagc gcaccattcg ccagttcggg ctggcgctcg agggcgccga tatcgcgctc
     7861 ttcttctatg ccggccacgg catccaggtc ggcggccaga accacctcat tcccgtcgat
     7921 gcgcggctgg cgagcgaggg cgacgtcgat tttgagaccc tgccgctgtc gctggtcttg
     7981 aagcagatgg agcgcgaagc caagaccagc ttggtgttgc tcgatgcctg ccgcgacaac
     8041 ccgttggcgc gcaacctggc gcgcagcatg ggcacgcgcg ccatcaccat cggcaccggg
     8101 ctcgccgagg tgaagacagg catcggcacc ctgatcgggt tttccacgca acccggcaac
     8161 gttgccgccg acggggtcgg ccgcaattcg ccctatgccg gggcgctgct caagcacatc
     8221 gaggattccg ccaaggacgt cagcggcatt ctggtcgacg tgcgcaacga cgtcttgaag
     8281 gccaccaacg gcacgcaagt gccctgggag cacacctcgc tgacggggca ggtctacttc
     8341 cgcctggagg ccccaccgcc ggggccggcg cctgccgcgc cgtcgggacg caactatgac
     8401 aaggagatgg aaatcgcgct gtgggatgcg gtcaaggaca gtaaaacgcc ggcgatcctg
     8461 aaaacctacc tcgatcgctt cccgaagggc acgtttgcgg gcctggcccg cgtcttgatg
     8521 gaggaagcca acaaggccgg tcgcagcggt gccgccccgg cggatccggg gtcctcgagc
     8581 ggcgcggcct ccccgagtgc cggcacttct gcccccaccg ccgtcagcaa ggatccccgc
     8641 gcgctggcgc gggccctgca ggcggaactg aaacgggtcg gctgcgatcc cggcgccatc
     8701 gacggcaact ggggtaaaaa ggcggaggac gccttggccg agttcgcgcg ccagagcaag
     8761 accacgctga cccgagaggc ctcgcaggag gcgctgaagg cggtggcggc gcagccgacg
     8821 cgcgtctgtc gcttggtttg cgggggcgac gaaatggagc gcgacggcaa gtgcgtcgcc
     8881 aaggcggcgc cggcgcgccg ccggccgcag gtggtggaga ccgcccccaa gcgcaagaag
     8941 gagagcacct cgggccagat gtgctggtcg aacgaccgcc gcaacatggc ggtggtgccg
     9001 tgcggggatg ccgcctcatc gggacaacgc gcctattgag cgtgccggcg ccggttaggc
     9061 cttggctggc ggtagcatgg cgttgtgctg gccgagcgtg ggcgcgcgcg tcaggatgcg
     9121 cgggcgctcc ccgtcgaagg agacgggcag gccgatcagg ttcagggcca ggtccggcac
     9181 cggctgcagg atgtcgaggg cggcggtctg tgggtggtcc ttcatgatgc tcatgtcgtt
     9241 gatcggcgca cagggcacgc cgatcttttc caggcgctcg acccattccg cactcgggcg
     9301 ttggcgcaat atctcggcga cggcgccgat cagcgatgcc ttgttggcga tgcgttgggg
     9361 gttggtcttg tagcgcgggt cattgaccca ctcgggatgg ccgagctcgc cggccagctt
     9421 ggcgaacagg cggtcgttgg ccgccgcgac gatcacctgg ccgtcggcgg tctcgaaacc
     9481 ctcgaagatc accaccccca ggctgccggt gggatgccgc tcgggcagct gcccgctcgc
     9541 ctggtagcgg gcaaagtgca cggtcaggag cccgagcgcg gtctcgaaca gggaggtatc
     9601 gacgattgcg cctttgcccg atcttgcccg ctcgagcagg ccggcaatgc agccaagcgc
     9661 ggtccacacg cccgtgccga ggtcgagcac ggaggtgccg atcctgaccc cgggccgatc
     9721 cgggtagcca ttgatggaga agaggccggc gaaggcctgc accattggtt cgtaccctgg
     9781 atttttctgc atcgggccgg tggggccgaa ggcggagagg ttgcaataga ccagccgggg
     9841 gttgagcgcg gtcatggcgg cgctgccgag ccccaaagcc tccatcacgc ccgggcgcag
     9901 gttgtgcagc agcacgtcgg cctcgccgat gtagcctttg agccaggcca gcgccgtgcc
     9961 gtccttgaga tcgagcgtga tgccgcgctt gttgaggttg agggcatgaa aggttgccga
    10021 ggtgtcgagc cagaagggcg gcccccaacc gcgcgcgtca tccccctcgg gccgctcgac
    10081 cttcagcacg tccgcgccca gcatcgccag gacgtagccc gcgtagggcc cggcgatgtt
    10141 gttggcgatc tcgacgacct ttacgccttt caaggggtgc atggccttgg ctcttttttt
    10201 gcgttgtggg gcaaaggggg cgtcagcggc cgctgaaatt cggcggccgc ttttccaggt
    10261 agtgggccac gccctcgcgg aaatcctccg tctcccggca gacctcctgc tcgcggttgg
    10321 cgatggcggt ggcctcggcc agcgattgga acaggccttc gtagacctgg cgcttgatga
    10381 tgcggaccga gcggggcgag gagagattgg caagctcggc ggcgcgttgc tgcacggctg
    10441 cagcgaagcc ctcggcaggc aagacgcgca ccaggcccag gcgctcggcc tcttccgccg
    10501 caacgacgcg cgccgacaac aacaggtcca tggcgtgcat ggggccgatc aggcgcggca
    10561 gcatccagga gatgccgtgc tcggcgatga ggccgcggcg ggcaaaggcg gtggtgagct
    10621 tggcgccggc ggccatgtag cggacgtcgc agtagagcgt gaggaccagg ccgatgccgg
    10681 ccaccgcgcc attgatgccg gcgatgatcg gtttgctgat ggccaggatg tagctgttgc
    10741 gctgggcgaa gttggcggcg agcccaacac ttggcgccgt ctccgagacc ttgaggctcg
    10801 tcttgccggc gacggcggcg gacaggcgcg ccatatccgc cccggcgcag aaaccgcgtc
    10861 ccgccccggt caagacgatg gcgcgcacgg cctcgtcttg atcggcgcgc gccagggcct
    10921 tgcgcagttc atcgcccatg ttcggcgtgt aggcattgag ccggtccggg cggttgagcg
    10981 tgatggtcgc cacccgctcg cggacttcgt agagaatgtc ctgatagacc tcgctcatcg
    11041 acgctctccc tgatcggccg tgcggcgcaa acggctttgc tcgcccattc gagcacgttc
    11101 gaaattttgc cgccagccga agctttagac cttcattttg ggatcgagcc agtcgcgcaa
    11161 ggcatcgccg aacaaattga agccgaacac ggcaagcgag atggcgacgc cgggaaagat
    11221 cgccacccac ggcgcgcttt ccgcatactc ttgggcgcct ccgcgcagca tcaggcccca
    11281 ggccggggtc ggctcctgca cgccgaggcc gaggaaggag agcgaggcct cgatcaggat
    11341 ggcctggccg acggccgccg tcatcatgat gatgtagggg gccatcacgt tgggcaccat
    11401 gtgccgcaag acgatacgcg tatgcgagaa gccgcaggcg cgcgcggcat cgatgaaggg
    11461 cgtctcgcgt accgccagcg cgctcgagcg caccacgcgg gcgcagcgcg ggatgaaggg
    11521 aatggtgatg gcaaggatga cgttttgcgc cccggtgccg aagatcgaga ccaccgccag
    11581 cgccaggatg acgagcggaa aggccatgac gatgtccatc acgcgctgga agatcaggtc
    11641 gaacaggcca ccgaaataag cactcgcgac ccccaggatc aggccgatgc tggcgccggc
    11701 gaaggccgag acaaagccaa ccagcaaggc tgtgcgcgcg ccgtagacga tgcgcgtgta
    11761 gagatcgcgc ccaaactggt cggtgcccag ccagtaatcg aggttgggcg cggtcagcat
    11821 ttcgccgaag ttattggcgg tcggatcgaa gcgcgtgatc tcttcggcaa agatggccat
    11881 gatcatcatg acgatcacga ccactccgcc accggccccc agcgggttct gcacgcagaa
    11941 gcgccacaac agctgccaga aggacgggcg tggcggtgcc tggtcgatcg ctgccccgcc
    12001 gcctggatgg gagatggttg ccatgccaaa ccggccttac ctgccgtagc ggatgcgcgg
    12061 gtcgagccag gcatagatca cgtcgaccac gagattggtg aaggcgaaga tggccaccac
    12121 cagcatcacc agcgcctggg tcatggtgta gtcttggttg gagacggact ccacaaacag
    12181 tttgccgagg ccgttgagat tgaagacctg ctcggtgacg accaggcctc ccatcaggaa
    12241 cgcgaactcg atgccgatga tggtgatgac gggcagcagc gcgttgcgca gggcatggcg
    12301 gcgcacgatc aagcggcgga acacgccctt ggcccgcgcg gtcctgacgt agtcctcgcg
    12361 caagacctcg agcatggccg accgcgtcat gcgcgtggcc accgccgaat aacgatagcc
    12421 cgtggccagc gccggccaaa tcagcatact gaggttatag acgggatcga cccagatcgg
    12481 cacgtatttg atgggtggca tccagggcga gccgaacaag gccttggagg tgatgagcat
    12541 ggccaagatg atgaggatgc cgagccagaa cgagggcatg gcgacgccgg cgatcgagaa
    12601 ggcgcgcacc aggtaatcga tccaggtgtt ctgcttcacc gccgagatgg tgccgagcgg
    12661 gatggcaagg gcgatcgcgg tcagcgtcgc catgatggcg acctgcagcg acagctggaa
    12721 gcgcagcccc acctcctcca cgatcggccg gccggtccac atcgagcggc cgaagtcgaa
    12781 ggtgatcagg cctttgataa aaaaccagaa ctgcatcagc agcggctggt ccatgccgag
    12841 ttccaagcgg caggccttca ccacgttcgg attgatgctg ccgccgcccg tggccagcct
    12901 gagcgtgcag atgtcaccgg gaatgagccg catcaaaaaa aacaccaacg ccgcggcgcc
    12961 aagcagcgtt gggatcatca gggtcaggcg cttgaagacg taaaagtaca tgggggctca
    13021 gggagcgtgc gccgtccagc gctggaccaa ccgcctattt gtcaagccac accatcgata
    13081 ggtcctggtt caggtagtgg ctaggcgaga tcttccagcc cttgaggtag gagcggtggg
    13141 gaatgatgcg ataccaccag aaggtgatga agttgtgcac ctcctcgtcg atggcgcgct
    13201 tttcgtaggc ccgcatcagc cggcgttgct cggccacgtc gccggtcctg ttcatggcct
    13261 cgtagagctt ggccatttcc ggatccttga agccgccgta ctgatcgccg gcgttgggca
    13321 ggaatttcga ggtatcgccc aggggattga ccaccgactg gcagttgaac tcgagtgcca
    13381 cgtcgaaagc gccgttttcc agggtggcat agaagggcgc cgtcggctcc atcttctgct
    13441 cgacgccgat gccgacctgt ttccattggt cgatcagcca ggtgccgaca atggtgtagg
    13501 gctggtcgac ggcgcggttg gcgagcgtga atttgagatc ggcggcgccg gcctccttga
    13561 ggaggcgttt ggcctcctcg cgcgcggcct tgatatcggt ggaatagccg gcgagcgccg
    13621 ccaattcctc cttgctcgcg gcgagcggat ggttggggaa gacgatgccg cccacggact
    13681 tgaccacggc gattttggaa aggtcgcgcg agccgctcca gcggtcgagt gccagggtaa
    13741 gcgccttgcg cacgcgcacg tcatcgaatg gcttcttgcg gctgttcggc gtgacgatca
    13801 gcaggcaatt ccagtcgctt tcctggaccg tgatgtcctt gccgagcgcg gccaccagat
    13861 cgtcgcggct ttttggcggc aagccgcgaa attcgatggc tgcctgatcg ccgcggatcg
    13921 cctgcacgcg cagcgattgc gtcttggcga aaatggcctc gaagccatcc aggtagggga
    13981 gcccggcgtg atagtagtcg ggattgcgtt tgccgcgcac cgccgcgccg gcttcgcggg
    14041 cctcgaacac aaaggggccc gagcccatca cgttctgctc gaaccagtgc ggatccttgt
    14101 cgaggatcgc cttggcgtag atgaagtgat agggcgaggc cagcgccggc aggaaagcgc
    14161 cggaggggta cttcagcttc atgacgaagg tttcgtcatc gggagccgac acgctctcga
    14221 ccatatggaa atgttctttg cgcgggctct gcacgccgtc gggcgggaag atgatgtgct
    14281 ggaaggaggc attgacgtcc ttggccgtca gcgccgaacc gtcatggaat ttgacaccct
    14341 tgcggat
//
LOCUS       DASTMT010000374         2034 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_5028712, whole genome shotgun sequence.
ACCESSION   DASTMT010000374 DASTMT010000000
VERSION     DASTMT010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2034
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5028712"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1684)
                     /gene="ligD"
                     /locus_tag="VFR00_08775"
     CDS             complement(<1..1684)
                     /gene="ligD"
                     /locus_tag="VFR00_08775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017956488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HEU0159395.1"
                     /translation="MAKTKLSPYQNKRDFTRTSEPSGSREVAAAEYPRFVIQKHAARR
                     LHFDLRLEIEGVFKSWAVTKGPSLDPKDKRLAVETEDHPLDYGDFEGTIPHGEYGGGT
                     VMLWDRGFWASDGQAAKALRQGELRFTLVGAKLQGGWVLVRLRRDRGKRTNWLLIKRH
                     DGYEREGDADAILQQDRSVASGRSMAEIAAGNGRSPTAFMLADKKAFTANAIWHSLSE
                     EAAVTPKDARASGARQRPPRRPRQSGLSMPDFIAPQLCEPVTRPPNGQDWVHEIKLDG
                     YRMQLRVEAGNAAMRTRKGLDWTGKFAAITAAGRALGNCIIDGEVVALDGNGASDFSA
                     LQAALAEGRADDLVYFAFDLLFADGQDLRSLPLLQRKERLSALLRGGGPHPSQVKFME
                     HLSEPGDRVLTSARALNLEGIVSKRANAPYVSGRTEGWRKIKCRGGQEVVIGGWSGSH
                     KHLRSLLVGVYRGDHLVHSGRVGTGFNARNAGDLLHKLVALQSDRSPFGGKGAPRRQA
                     DWHWVKPQLVAEIEFAGWTADGNVRQAAFKGLRDDRPAEEIRAERSAMTATAE"
BASE COUNT          352 a          700 c          623 g          359 t
ORIGIN      
        1 gctccgccgt tgccgtcatt gccgaccgtt cggcccggat ctcctcggcc ggtcgatcgt
       61 cgcgcaaacc tttgaaggcg gcctgccgca cattgccatc cgccgtccag ccggcgaact
      121 cgatttccgc gaccagctgc ggtttgaccc agtgccagtc cgcctgtcgc cgcggcgcac
      181 ccttgccgcc gaaggggctt cgatcactct ggagtgccac cagcttgtgc aggaggtcgc
      241 cggcattgcg cgcgttgaag ccggtgccga cgcggccgct atgaacgagg tggtcaccgc
      301 ggtagacgcc gacgagcagc gagcgcaggt gcttatgact gccgctccag ccgccgatca
      361 ccacttcctg tccgccgcga cacttgattt tccgccagcc ctcggtgcgc ccggagacgt
      421 agggcgcgtt tgcccgtttc gacacaatgc cttcgagatt gagggcgcgg gccgacgtca
      481 acacccgatc gcccggctcc gacaggtgct ccatgaattt gacctggctc ggatgggggc
      541 ccccacctcg caatagcgcg ctcaaacgct ccttgcgttg gagaagcggt agcgaccgca
      601 aatcctggcc gtcggcaaac aacagatcga atgcgaaata gacgaggtcg tcggcacgac
      661 cttccgccaa tgccgcctgc agcgcagaaa aatccgatgc accattgcca tcgagtgcaa
      721 ccacctcgcc gtcgatgatg cagttgccta aagcgcggcc cgcggccgtg atggcggcga
      781 atttgccggt ccaatcaagg cccttgcgcg tgcgcatggc cgcattgccc gcctcgacgc
      841 gcaactgcat gcgataacca tcaagtttga tctcatgcac ccaatcctgg ccattgggtg
      901 ggcgggtgac cggctcgcac agctgcggtg caatgaaatc cggcatgctt agcccagatt
      961 gccgtgggcg gcgcggcggc cgctgcctcg ccccggaagc ccgcgcgtct ttcggcgtga
     1021 cggcggcctc ctcgctcagc gaatgccaaa tggcgttcgc cgtgaaggcc ttcttgtcgg
     1081 caagcatgaa ggccgtgggg ctgcgcccgt tgccggccgc gatctccgcc atgctgcggc
     1141 cggatgccac cgaccggtcc tgctgtagga tggcatcggc atcaccttcg cgctcatagc
     1201 cgtcgtgacg tttgatcagc agccaattgg tgcgcttgcc gcgatcgcga cgcaggcgca
     1261 ccaataccca accgccttgc aacttcgcgc caaccagtgt gaagcggagc tcgccctggc
     1321 gcagagcctt cgccgcctgc ccatcgctcg cccagaagcc gcgatcccaa agcatgaccg
     1381 tgccaccgcc atactcgccg tgcggaatgg ttccttcgaa gtcgccgtaa tcgagcggat
     1441 ggtcttcggt ctcgacggcg agccgtttgt ccttgggatc gagcgaaggc cccttggtca
     1501 cggcccaaga cttgaagacg ccttcaatct caagacgaag gtcgaaatgc aggcgccgcg
     1561 ccgcgtgctt ctggatgacg aagcgtggat actcggccgc tgcgacctct cggctgccgc
     1621 ttggctcact cgtacgcgta aaatcgcgtt tgttttgata gggtgagagc ttggtcttcg
     1681 ccatcactgg tcctgctacg ccccgagcaa cggcttggta acgtcttgct gcccccacgt
     1741 tcacccaacc gcgcgccaat tggaaggcga ccggcgcctt aaccgatgcc ggcggttaac
     1801 cgctcgcgcc ggcggtgcgg gcagcgcacg ccaagccgat gcaaagcccc tcgcttgggc
     1861 gtggggcggc agcaaacatg gcaccaagac gagaagggcc acccttttgc cctgcggcaa
     1921 cgccgctcgc cctgccaatc acgcctgttc gatgccatgg caggcgcgcc gcccaccgac
     1981 acccgtcaag gccgggacgc ggacgctgaa acgagcacgg gattggggcg gtaa
//
LOCUS       DASTMT010000375         4464 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_2497526, whole genome shotgun sequence.
ACCESSION   DASTMT010000375 DASTMT010000000
VERSION     DASTMT010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4464)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4464)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4464
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2497526"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..165
                     /locus_tag="VFR00_08780"
     CDS             <1..165
                     /locus_tag="VFR00_08780"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HEU0159396.1"
                     /translation="DERLLKGGSYLCHRSYCYRYRIAARTGAAADSSTGHVGFRLVFD
                     DYPRSFPNTP"
     gene            171..1610
                     /locus_tag="VFR00_08785"
     CDS             171..1610
                     /locus_tag="VFR00_08785"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEU0159397.1"
                     /translation="MQPTNLLVIMSDEHHPRAMGVAGHPFAITPHLDRLAARGTRFTA
                     AYCNSPICVPSRASFATGRYVHELGTWDNALAYDGRIPSWGHALQRSGHRVDAIGKLH
                     YRCADDPTGFDHQYHPMHIYGGHGMVWGALRDGKADFTERAHAMLNPIGPGTSKYNIY
                     DDRIATEAENWLARRARAPQERPWVLFVGFVAPHFPLTVPQHILDLYPAERMPLPRLH
                     PDRGHPRHPWLEAQHRMHPVDAALDDGGRRLATACYLGLCTWVDAQVGRIIAALQKTG
                     LAETTRVIYTSDHGDNVGARGMWGKSTHYDDAVGVPMLIAGPGIAQGHLCSTAVSLVD
                     LAPTILAAVGEDPYAWSPDLPGRSLFEIAEAPYDPQRTVFSEYHAFASPSAAFMLRNG
                     RYKLNYYVGYRPELFDLSNDPLEEHDLGRSAEHAAVRAALESELRAMLDPEATDARAK
                     ADQAALIERYGGPAKALHIGAPGATPAPL"
     gene            complement(1779..2468)
                     /locus_tag="VFR00_08790"
     CDS             complement(1779..2468)
                     /locus_tag="VFR00_08790"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU0159398.1"
                     /translation="MDWAEYWNQETSLYVNERHRRLHYQQVTRDIITHVPASDIRLMD
                     YGCGDTPAARDLAAACGHLLLCDAAPRVRDELAARYAGECKISILTPDQFQALPAASL
                     GMVVVNSVVQYLSRSEFAALLQAARHKLEPGGVLLLADIVPPHLSPWRDALELLKFAA
                     SGGFLVPAVVGLARSYVSPYRKIRQRSGFLQFEEAELLALLRQHGFEAQRQPRNIGHN
                     HERMTFRAIAG"
     gene            3019..>4464
                     /locus_tag="VFR00_08795"
     CDS             3019..>4464
                     /locus_tag="VFR00_08795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0159399.1"
                     /translation="MTELGVSTVSAGRRSRLAIGSEAQFLRLLVILAGLVWSLLFIVV
                     GLYYDLQLFGDGSIFSYSVAVADGWRIHWHNISCRLFVLLFCHVPAEAYVKLTGDPRG
                     GIAIYGFLFFGAQLFGLIATFALDRSRQRILFTYACGSTAVLCPLVFGFPTEMWIAHA
                     LFWPTLAIVHYGRGPLAGPVSFLAFLCLVLCHEGALIFAAVILATLALRGPRDPALVR
                     SATAGVAALAIWIAIRLELPPDAYIAGVLANAALNFIDLSSLTLNPMRLLLVALAGYG
                     LALLALTWAKLERSHFYAALAVVAVLSVHELTADAPIIGENRYFLRTALLTFTPIFGA
                     LAAACAVAAEGRLVLALPYLRPALGVLARTKTVRMAAGALLVAMSVHVLETERFVAAW
                     ISYEDAVRKLATGTTSDPALGDPRFVSANRIPERLNGLVWASTTHFLSILLAPAFAPA
                     RLVVDPDTNYFWLPCRTAAANSVGRRAVPLVT"
BASE COUNT          676 a         1577 c         1400 g          811 t
ORIGIN      
        1 gacgagcgcc ttctcaaggg cgggtcgtac ttgtgtcatc gttcctattg ctatcgctac
       61 cgcattgccg cccgcaccgg ggcggcggcc gacagcagca ccggacacgt cggcttccgc
      121 ctggtcttcg acgattaccc ccgctccttc cctaacaccc cctgagaacc atgcagccga
      181 ccaacctcct cgtcatcatg tcggatgaac atcacccccg cgccatgggc gtggcgggcc
      241 atcccttcgc catcacgccc cacctcgacc ggcttgccgc acgcggcacg cgcttcaccg
      301 ccgcctactg caactcgccg atttgcgtgc catcgcgcgc atccttcgcc accggtcgct
      361 acgtgcacga gctcgggacg tgggacaacg ctttggctta cgacggcaga attcccagtt
      421 ggggccacgc gctacagaga agcggccacc gcgtcgatgc gatcggcaag ctgcactatc
      481 gatgtgccga cgatcccacc ggcttcgatc accaatacca ccccatgcat atctacggcg
      541 gccatggcat ggtctggggc gccttgcgcg atggcaaagc cgacttcact gaacgggcgc
      601 acgcgatgtt gaacccgatc ggtcccggca cctcgaaata caatatctac gacgaccgca
      661 tcgccaccga ggcggagaac tggctcgcgc gccgcgcgcg cgcgccgcag gaacgcccgt
      721 gggtgctgtt cgtcggcttc gtggcgcctc actttcccct gacggtaccg cagcacattc
      781 tcgatctcta tccagccgaa cgcatgcccc tgccgcggct acaccccgac cgcggccatc
      841 cccgccaccc ctggctggaa gcccagcacc ggatgcaccc cgtcgatgct gccctggacg
      901 atggcggccg ccgcctggcg accgcctgct acttgggcct gtgcacgtgg gtggacgcgc
      961 aggtcggtcg cattatcgcg gcgttgcaga aaaccggtct cgccgagacg acacgcgtca
     1021 tctacaccag cgaccacggc gacaacgtcg gcgcgcgtgg tatgtggggc aagagcacgc
     1081 actacgacga cgcggtaggg gtgccaatgc tcatcgccgg gccggggatc gcgcagggac
     1141 acctatgctc gacggccgtc agcctcgtcg atctcgcccc caccatcttg gccgccgtcg
     1201 gcgaggaccc ttacgcctgg agcccggatc tgcccggtcg ctccttgttc gagatcgccg
     1261 aggcgcctta cgacccgcag cgcaccgtgt tttccgagta ccatgctttt gcctcgccat
     1321 ccgccgcctt catgctgcgc aatggccgct acaagttgaa ctactacgtg ggctatcggc
     1381 ccgagctctt cgacctcagc aacgacccct tggaagagca cgacttgggg aggagcgccg
     1441 agcacgcggc cgtccgggcg gcgttggaat ccgagctgcg cgcaatgctg gacccggagg
     1501 caaccgacgc gcgcgccaaa gcggatcagg ccgcgctgat tgaacgctac ggcggacctg
     1561 ccaaggcctt gcatattggt gctccaggcg ccacgccggc acccctgtga cgccctcgcg
     1621 agggccggga aagcgcaaat ttcaaggcct tcagggttga ggtgacctgg ccaaccccag
     1681 cgcgcgcggg acgaagagga cggccgccgc agccgcccac agagaacccc atgtcccgcg
     1741 gccctcggcc cgagagagag gagacgcgag gcggacgctc aaccggcaat ggcgcgaaag
     1801 gtcatgcgct cgtggttgtg gccaatgttg cgcggctggc gctgcgcctc gaagccgtgc
     1861 tggcgcaaga gggccaagag ttctgcctcc tcgaactgca aaaagccgga ccgttgtcgg
     1921 atcttgcgat agggcgagac gtaagagcgt gccaggccga caacggccgg cacgagaaag
     1981 ccgccgctgg cggcgaactt gaggagttcg agcgcgtcgc gccaggggct gaggtggggc
     2041 ggcacgatgt cggccaaaag cagcacgccg ccgggctcga gcttgtggcg cgcggcctgg
     2101 aggagcgcgg caaactcgga cctcgacagg tactgaacca cagagttgac caccaccatc
     2161 ccgagcgaag ctgccggcag ggcctggaac tgatctggcg tcagaatgct gatcttgcac
     2221 tcgccggcgt agcgcgccgc caattcgtcg cggacgcggg gggcggcatc gcagagcaat
     2281 aagtgaccgc aggcagcggc gagatcgcgc gccgccggcg tatcgccaca gccatagtcc
     2341 atgaggcgta tgtcgctcgc cggcacatgc gtgatgatat cgcgcgtcac ctgctgataa
     2401 tgcaggcgtc ggtggcgctc gttcacgtag agcgacgtct cctggttcca atactcagcc
     2461 caatccacgt cccacatcca ttctgcagcg gcgccgcacg accccgctga ccatctcggc
     2521 cctgcgagca tcggcgggcc aagcccccca gcgcccatgc ccgtcgctgc gcgctcaggc
     2581 ggccgctgcc gtcccgccga ctgcgccgct tggcgcctcg cagcttttcc gagccctcgc
     2641 gccggcgttc cgccggcgcc gctcaagcag ccgaggggtg cgcgcggcct cagcaggccg
     2701 gcatttgctt cacttctttg ccactcgtgt cgcagatggg caacggttcg ggcgcacgac
     2761 cttggtgcgg cggcgcaaca tcgttcttgt cgggcaagga ggggcgtgtc ggcagagcac
     2821 ggtcagctat tttgcaagat ctgctcgttt tgacgcgttg cgtggaatcg ggtgccgccc
     2881 taagcgtcgt atcgacatgc ttacgcaacc gattctcgcc gataccgccg cgcagtcctt
     2941 ccctggcgct cggctcgggt gggctaggcg gcgcccgccc gccggcttgc gacatgccgc
     3001 atccgcacgg cctctgccat gacggagctg ggcgtcagca ccgtctcggc cggccggcgc
     3061 agccggctcg ccatcggctc ggaggcgcag tttctgcgcc tgttggtgat cctggccggg
     3121 ctggtgtggt ccctcctctt catcgtggtg gggctgtact atgacctgca gctgttcggc
     3181 gatggctcga tcttctccta ttcggtggcg gtggccgatg gctggcgcat tcactggcac
     3241 aacatctcct gccgcctctt cgtcttgctg ttctgccacg tgcccgccga ggcctatgtc
     3301 aagctgaccg gcgatccgcg tggcggcatc gccatctatg gcttcttgtt ctttggcgcc
     3361 cagctgtttg gtttgattgc taccttcgcg ctggaccgct cacgtcagcg catcttgttt
     3421 acctacgcct gcggctcgac ggcggtgctg tgcccgctcg tgttcggctt cccaacggag
     3481 atgtggatcg cacatgcctt gttctggccg acgctcgcca tcgtgcacta tggccgtggt
     3541 ccgctcgccg ggcctgtctc ctttttggcc ttcctgtgcc tcgttctgtg ccatgaggga
     3601 gcgctcatct ttgccgcggt gatcctggcc acgctcgctc tgcgcgggcc gcgcgatccc
     3661 gccctggtgc gctcggcgac ggcgggagtg gcagcgctcg ccatctggat cgcgatcagg
     3721 ctcgagttgc cccccgacgc ctatatcgcc ggcgttctcg ccaatgcggc gctcaacttc
     3781 atcgacctca gcagcctcac gctgaacccc atgcgccttt tgctggtggc gctggccggt
     3841 tacggcctgg cgctgctggc attgacctgg gccaaactcg aacgctcgca tttttatgcc
     3901 gccttagcgg tggtggcggt cctcagcgtg catgagctca cggccgacgc cccaatcatc
     3961 ggggaaaatc gctattttct gcggaccgcg cttttgacct tcacgccgat cttcggggcg
     4021 ctcgcggcgg cttgcgcggt ggccgcggag gggcgcctcg tcctggcgct accctatctg
     4081 cggccagcgc tgggggtcct tgcccgcacc aagaccgtcc gcatggcggc gggcgcgctc
     4141 ctggtggcga tgtccgtgca cgtccttgag acggaacgat tcgttgccgc ctggatcagc
     4201 tacgaagacg ccgtgcgcaa gcttgccacc ggcacgacgt ccgatccggc gctcggcgat
     4261 ccgcgttttg tttcggccaa ccggatcccg gaacgactga acggcctggt gtgggcctcc
     4321 accacccatt tcctctccat cctgcttgcc cccgcctttg cccccgcgcg cctggtggtc
     4381 gaccccgaca ccaactattt ctggctgccc tgcaggaccg ccgccgccaa cagcgtgggc
     4441 cggcgcgccg tgccgctcgt gacg
//
LOCUS       DASTMT010000376        11837 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_1192019, whole genome shotgun sequence.
ACCESSION   DASTMT010000376 DASTMT010000000
VERSION     DASTMT010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 11837)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11837)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11837
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1192019"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..403)
                     /locus_tag="VFR00_08800"
     CDS             complement(<1..403)
                     /locus_tag="VFR00_08800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0159400.1"
                     /translation="MTLNYRLGTLPVALLLAAVPAVALAAVDCRAPRMAAADLAICTD
                     PQLARLDDQLTRRLQKTARQLAFGPYVGLRIWQSDWRQQRSECSADRACLTASYLDAN
                     RFLDRFQRCLGTSLRGRRCLPVSVEGERAAAR"
     gene            complement(563..910)
                     /locus_tag="VFR00_08805"
     CDS             complement(563..910)
                     /locus_tag="VFR00_08805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0159401.1"
                     /translation="MTVKGGRRTGRSVPLGMLALGLVLLVPAAAEAKRIKIRARPSLG
                     AGWAQSGAPPTGAANEAAHVSPSTSPPGRAEAADARARAALAAERARWSAAASMSAAP
                     VSPDGGMVCLAGC"
     gene            complement(937..1374)
                     /locus_tag="VFR00_08810"
     CDS             complement(937..1374)
                     /locus_tag="VFR00_08810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0159402.1"
                     /translation="MRTSYRGLRAAQMAALFLAVALISAAPVCAKDGRPPTAACEACA
                     APRPWRAFERLGHDAALDEEDEIAALEAIRVALSEVGDGVSYVWHRRYGQLGGLVQPT
                     HSFIDVHGRVCRSIIVILSAGPRSGRIEGTACRLAGGRWQLEG"
     gene            1493..2140
                     /gene="pdxH"
                     /locus_tag="VFR00_08815"
     CDS             1493..2140
                     /gene="pdxH"
                     /locus_tag="VFR00_08815"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008935395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HEU0159403.1"
                     /translation="MTDEKAHAVPGQPAGDFTQAQDPFGLFETWFAEASRSEQSDPNA
                     MALATVDTAGLPNVRIVLLKGLDAPSHPARGFVFYTNLESAKGGELISARVAALNFHW
                     KSLRRQVRARGTVTVVEEAEADAYFATRPRGSRIGAWASQQSRPLESRYALEKAVAAI
                     TAKYLIGEIPRPAYWSGFRLTPLAMEFWHDRPFRLHERVAFKRTDGGWRKELLYP"
     gene            complement(2137..2400)
                     /locus_tag="VFR00_08820"
     CDS             complement(2137..2400)
                     /locus_tag="VFR00_08820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018043507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3297 family protein"
                     /protein_id="HEU0159404.1"
                     /translation="MHDSPPDRLSANELSPYYDAAALERGVGVRFKGVEKNNVEEYCV
                     SEGWVRLAVGNTKDRHGRPLTIKLVGKVEPYFRTPAGGGAAQD"
     gene            complement(2491..3435)
                     /locus_tag="VFR00_08825"
     CDS             complement(2491..3435)
                     /locus_tag="VFR00_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HEU0159405.1"
                     /translation="MPKTHRLDAGPETVHWGYFDAALRPLLSVESGDTVIISTVSGGR
                     EVMPAPPLVIPPALSAIHAAQRPKLPGHICTGPVAIKGAKPGQVLEVRINEISLNYDW
                     GYNFSGPLTGALPDDFPERHLMHITLDRQRMLGRLPWGGELALRPFFGVMAVAPPPAW
                     GSVSTLPPRRNGGNLDNKELVAGTTLYLPIQVEDALFSVGDGHGVQGDGEVCVTAIET
                     GLIGTFQLILRDDLRLEWPRAETPTHIMTMAFDPDLDTCVVIALRQMLELIANQTGLS
                     RQQAYALCSLAADVRVTQVVNGAKGVHVMLEKRHLTPI"
     gene            3714..5726
                     /locus_tag="VFR00_08830"
     CDS             3714..5726
                     /locus_tag="VFR00_08830"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HEU0159406.1"
                     /translation="MKPTPKRARSAAVLKPTLFQRLPWEMRLTYLAAIYLGLPLALLL
                     VGAFATFNATIPDPIQLRHKESAPLVRILARDGSLLAQRGGEGSYVPIDFLPPHLIDA
                     VVATEDRRFFEHWGVDLAGVARALITNLRLGRLVQGGSTISQQLAKNLFLGPERTLTR
                     KIGELVLALWLEFRLGKRDILELYLNRVYFGAGAWGIDAAAHRFFDKSAQALSLAESA
                     VLAGLLKAPSRFSPASNPVLARARARTVLSKMVEAGRLDPVRGEQVARAPFRFAALAR
                     QRQAGADYAVDAALERLPTLIASAGEADLIVETSIDAGLQRKSQALVAELMRGEARAL
                     EASQAALVVLDLDGGIRTLVGGRDYAESQFNRALKAKRQPGSAFKTFVYLAALESGLT
                     PDSIVQDLPLLGTGWSPRNEGAGYRGAVPLREALAQSMNAAAVRLHLSLTPSRTLTAA
                     RRLGIRSELRQDASLALGTSEVSLLELTGAYGVIAHGGRALEPYLIRRVRTAAGEVLF
                     ERRAEPSTTLIAPNIAGALSDMLLGVVSRGTGRRAALAEHAVAGKTGTTQDFKDAWFV
                     GYTAHLVAGVWVGNDDARPMRKVTGGSLPARLWHDVMSIAHEGLPPRALPGSPPSERA
                     GLGPSRALDGAGATRPALQRGVPEFFAGARAGAEPAPAVTAQSVVR"
     gene            complement(5708..6469)
                     /locus_tag="VFR00_08835"
     CDS             complement(5708..6469)
                     /locus_tag="VFR00_08835"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015596240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HEU0159407.1"
                     /translation="MALRVHGISAPVEVRRHPGARRLTLRVSNTRRWVVVTLPAQCRM
                     EEADRFLRSHIDWVRERLGSVPEPVPFVDGALIPLRGGMHRLRFLGPRPGAAVVRISR
                     APGKTPTLEVSGRGEHAARRLTDWLMGEARADLDACVSRHARALGVRVRRLCLRDQTT
                     RWGSCSTSGLLSFSWRLILAPAHVLDYVAAHEVAHLREMNHGPKFWTLVARLVPELEA
                     ARRWLRHHGADLHRYGADELGPPVTPPLKFQRTTL"
     gene            complement(6676..8016)
                     /gene="phaZ"
                     /locus_tag="VFR00_08840"
     CDS             complement(6676..8016)
                     /gene="phaZ"
                     /locus_tag="VFR00_08840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate depolymerase"
                     /protein_id="HEU0159408.1"
                     /translation="MLYHWYELGRAACRPARAAADSCRLFFNPLNPLTHTPVGRSAVA
                     ACEVFERATRRYTKPAFGITQAQVRGRLVKVEEEEVWRTPFCRLVRFRRDIDVSEGAR
                     DPRLLLVAPMSGHFATLLRGTVETFLPDHDVYITDWQDARMVAQAAGSFDLDNYIDAM
                     IAIFRFLKGDVHVFAVCQPAVPVLAATALMEEDAEADVPLSLVLAGGPIDTRINQTVV
                     NELAERRGTDWFRRTVITNVPWPNPGHGRLVYPGFLQLSGFMTMNLDRHLEAQKEMFL
                     HLVRGDGDSAEKHREFYDEYLAVMDLTAEFYLQTVDTVFVKHLLPRGLMTHRGRPIRL
                     GAIRRPALMTVEGEKDDITGTGQCHAALALCTGIAEADKRHFECPRVGHYGIFNGSRF
                     RAQIAPRISAFMRAHDPCSVEGLSIAQLEHMAAMPRRRRSSADFDSLAFSFAAR"
     gene            complement(8167..8784)
                     /locus_tag="VFR00_08845"
     CDS             complement(8167..8784)
                     /locus_tag="VFR00_08845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031966 - mitochondrial membrane
                     [Evidence IEA];
                     GO_process: GO:0033617 - mitochondrial cytochrome c
                     oxidase assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEU0159409.1"
                     /translation="MTNSSLRLALIVVAGLLVGAAAALAIFPAARERLLPAGAPSVGH
                     ALVGGPFSLTDQTGKRVTDNDFRGRYLLVFFGFTNCPDVCPSALQVMAAALDKLGPKG
                     QKITPLFISVDPERDTPTQLATYVKSFHPRLVGLSGTPAEIEAVTKAYRVYVKKVPDP
                     KSSAGYTFDHSAIIYLMGPDGAYLAHFTHASNADSMAERLAKLLH"
     gene            complement(8797..9564)
                     /locus_tag="VFR00_08850"
     CDS             complement(8797..9564)
                     /locus_tag="VFR00_08850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016845517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0159410.1"
                     /translation="MILRYWYVIRSSWPRTAELIYWPLVQMLMWGFLQSYLASTSSFA
                     AKAAGLFIGAVLLWDILVRSQLGFAVAFLEEIWSRNLGHLMLSPLRPGELVAALMAVS
                     FIKLMVAMVPVAILAYTFFGFNVLSLGSAFAAFFANLVLTSWALGLVSTGVVLRWGLG
                     AESFAWLIVFVFLPLCCVYYPVDTLPGWLQGVALALPPTHVFEGLRAIVLEGRFVAAS
                     MLMAFVLNVIYLALGFAAFGYFLQSARRHGTLVQMGE"
     gene            complement(9648..10430)
                     /locus_tag="VFR00_08855"
     CDS             complement(9648..10430)
                     /locus_tag="VFR00_08855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0159411.1"
                     /translation="MQRPIPPPPGAEPPPVEVVRLRKVYRDVAAVDGLDFKLTAGAVT
                     ALLGGNGAGKTTTISMLMGLVIPTSGQARVFGADMARERYRVLHRMNFESPYVDVPMR
                     LTVRQNLEVYGRLYGVRQLKSQVAAIAADLRLSEFLDRPYGKLSAGQKTRVSLAKALL
                     NEPELLLLDEPTASLDPDTADWVRAKLQHYLRARRATVVLASHNMAEVERLADRVIML
                     EKGRVIADETPAQLIERFGRSTLEEVFLAIARRSRANEESAA"
     gene            10599..>11837
                     /locus_tag="VFR00_08860"
     CDS             10599..>11837
                     /locus_tag="VFR00_08860"
                     /inference="COORDINATES: protein motif:HMM:NF033792.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ActS/PrrB/RegB family redox-sensitive histidine
                     kinase"
                     /protein_id="HEU0159412.1"
                     /translation="MLRRRERPWDETAGSRLRLQTAVRLRWFGVIGQLLTVGVVYLLL
                     GFQLPLGICLAFIALSAWLNVFLRIRFPARTRLSTGFATSLLAYDILQLAALLFLTGG
                     ADNPFIFLLVAPVTVSAATLPPRNTIALGLLGAGMAVLLLFYSMPLPWYPGSRPDLPM
                     LYSVGVLASVLSGMVFLALYAWRLAKEARQMSDALAATELVLAREQQLHALDGLAAAA
                     AHELGTPLSTIAVIAKELAREPAAGGQFADDIALLQAQAMRCREILTKLTRSPSEPDP
                     LHARMSVTQLIDEAAEPYRSTSSLSISAEPEAGVDGEAALEPVGERKPGVIYGLSNLI
                     ENAVDFARQRVEIVARWSARDVLLTISDDGPGIPLDVLDALGEPYITTRGSRRAGQIK
                     NGEPAGLGLGFFSAKTLLERS"
BASE COUNT         2005 a         4004 c         3971 g         1857 t
ORIGIN      
        1 ggcgcgccgc ggcgcgctcg ccctcaacgc tcaccggcag gcaccggcgc ccgcgcaggc
       61 tggtgccgag acagcgctga aagcggtcaa gaaagcggtt ggcatcgagg taagaggccg
      121 tgaggcaggc gcggtcggcg ctgcactcgg agcgctgctg acgccaatcg ctttgccaaa
      181 tgcgcaaacc gacatagggg ccgaaggcga gctgccgcgc cgtcttctgc agacgccggg
      241 tcagctggtc atccaggcgc gccagctgcg gatcggtgca aatcgccaaa tccgcggccg
      301 ccatccgggg cgcgcggcag tcgacggcag ccagcgcaac ggcagggacc gcggcgagca
      361 gcagggcgac agggagcgtg ccaagacgat agttcagggt catgcgcagg gccgtgctcg
      421 atcgtgaacg gctcccccat ctctgcccgc tcccgcggcg ttggtccagt gcttgttgcg
      481 gtcgcgcgcg aggtttttac cggcgcgccg gaagcctctg ggaaaatcgc cgcaccggca
      541 gcaaggcctt agagcctgcc gctcagcacc ccgccaggca gaccatgccg ccgtcggggc
      601 tcaccggcgc ggcgctcatg ctcgccgcgg cgctccagcg ggcacgctcg gcggccaggg
      661 ccgcgcgggc gcgggcgtcg gccgcttcgg ctcgccccgg tgggctcgtg ctgggggaga
      721 catgcgccgc ctcgttggcc gcccccgtgg gaggcgcgcc actttgggcc cagccggcgc
      781 ccagactcgg ccgcgcacgg atcttgatgc gcttggcctc cgccgcggcc ggcacgagca
      841 gcaccaggcc cagcgccaac atccccagcg gcacgcttcg gccggtgcgc cttccaccct
      901 tgacggtcac gttttcctcc cgttcgcgaa cggcgcttag ccttcgagct gccagcggcc
      961 gcctgccaga cggcaggcgg tgccctcaat tcgcccggaa cgcggacccg cgctcaggat
     1021 cacgataatg ctgcggcaga cgcggccgtg cacatcgatg aaggaatgcg tcggttgcac
     1081 caggccgccc agctggccat agcggcgatg ccagacatag gagaccccgt cgcccacttc
     1141 cgagagcgcg acgcggatcg cctccagcgc ggcgatctcg tcctcctcat cgagtgcggc
     1201 gtcgtggccg aggcgttcaa aggcgcgcca ggggcgcggc gcggcgcagg cctcgcaagc
     1261 agccgtcggc ggtcgcccgt ccttggcaca gaccggcgcg gcggagatga gagcaacggc
     1321 aagaaagagg gcggccattt gagccgcacg caagccgcga tatgatgtac gcatgaccaa
     1381 ctcactcgtc accacgcccg tgcgggcggg ctcacaaccc ccgagcaacg ctgcgccgaa
     1441 ctgtggacca gcgtggtaaa aaagccgtaa aggccaactg cgagcgacgc cgatgaccga
     1501 cgagaaagcg cacgccgtac cagggcagcc ggccggcgat ttcacccagg cgcaagatcc
     1561 gtttggcttg ttcgagacct ggtttgccga ggccagccgc agcgaacaga gcgaccccaa
     1621 cgcgatggca ctggccaccg tggatacggc cgggctcccc aacgtgcgca tcgtcctgct
     1681 gaaaggtctc gatgcgccga gccatcccgc gcgcggcttt gtgttctaca ccaacttgga
     1741 aagcgccaag ggcggcgagc tgatctcggc gcgagtcgcc gccctcaact ttcactggaa
     1801 atcgctgcgc cgccaggtgc gcgcccgcgg caccgtgacg gtggtagagg aggccgaggc
     1861 ggatgcctat ttcgccacgc ggccgcgcgg cagccgcatc ggcgcctggg cctcacagca
     1921 atcccgaccg ctcgagagcc gttatgcgct cgagaaggcg gtggccgcca tcaccgccaa
     1981 gtatctgatc ggcgagattc cgcgccctgc ctattggtcg ggctttcgcc tcaccccgct
     2041 ggcgatggag ttctggcacg accgcccgtt ccgcctgcac gagcgcgtcg ccttcaagcg
     2101 cacggatggc ggctggcgca aagagctgct ttatccttag tcctgcgccg cgccgccgcc
     2161 tgcaggggtg cgaaaatagg gctcgacctt gcccacgagc ttgatcgtga gcggtcgccc
     2221 gtgccggtcc ttggtgttgc cgacggccag ccgcacccag ccttcgctga cgcagtattc
     2281 ctcgacgttg tttttctcca cgcccttgaa gcgcacgccg acgccgcgct cgagcgcggc
     2341 cgcgtcgtag taggggctca gttcattggc cgagagccga tcgggcgggc tgtcgtgcat
     2401 cggggcggac cttcgcaaaa aacgcctggc ctcatttatc gagccgcggc ggcgagggca
     2461 atgcgggccc cagccttacg gcacagcggg tcaaatcggc gtcaggtgac gcttttccag
     2521 catgacgtgg acacccttgg cgccgttcac gacctgcgtc acgcgcacat cggcggccag
     2581 gctgcagagc gcgtaggcct gttgcctaga aagcccggtt tggttggcga tcagctccag
     2641 catctgacgc aaggcaatga cgacgcaggt gtcgaggtcg ggatcgaaag ccatggtcat
     2701 gatgtgggtc ggggtctccg cgcgcggcca ttcgagccgc agatcgtcgc gcaggatcag
     2761 ttggaaggtt ccgatcaatc cggtttcgat cgcggtgacg cacacttcgc cgtcgccctg
     2821 cacgccgtgc ccgtcgccga ccgagaacag ggcgtcctcg acctggatcg gcaaatagag
     2881 cgtcgtgcct gcgaccaatt ccttgttatc gaggttaccg ccgttgcggc gcggcggcag
     2941 cgtcgaaacc gatccccagg cgggcggcgg cgccacggcc atgacgccga agaaggggcg
     3001 caaggcgagc tcgccacccc agggcagccg cccgagcatg cgctgccggt caagcgtgat
     3061 gtgcatgagg tgacgctcgg ggaaatcgtc aggcaacgcg cccgtcaatg gccccgaaaa
     3121 attataaccc cagtcgtagt tgaggctgat ctcgttgatg cggacctcga gcacctgccc
     3181 gggcttggcc cccttgatgg ccacgggccc cgtgcagatg tgacccggca gcttgggccg
     3241 ttgcgcggcg tgaatggcgc tgagcgccgg cgggatgaca agcggcgggg ccggcatcac
     3301 ctcgcggccc cccgaaacgg tggagatgat taccgtgtcg ccgctttcga cgctgagtaa
     3361 ggggcgcagc gcggcgtcga agtagcccca atgcacggtc tcgggcccgg catcgagacg
     3421 atgggtcttt ggcatagagc aggcctcccc atgttgctgg cgccatagcc tagcgcgtcc
     3481 gcggccgggc ggcgatgggg cgggcgccga cgcacaccaa cccgcagcac ggcttgccac
     3541 ccgcgcgccg cgccgggggg cgccttcgcg cacggttaac cgggccttaa ggcgcgcgcc
     3601 ctagccttgg gacggccggc ttggaaccgg cacgcggcgc gcacgccgcg cgcgtgcgtc
     3661 aagggttggc ggcaggcatg ggactggggc gtcggtctcg agcacggcag gctgtgaagc
     3721 caacgccaaa gcgcgcccgc agcgcggcgg tcctcaagcc aacgctgttc cagcgcttgc
     3781 cctgggagat gcgcctcacg tatctcgcgg ccatctacct cgggcttccc ctcgcgctgc
     3841 tgctcgttgg cgcgtttgcc accttcaatg ccacgattcc cgatccgatc cagctgcgcc
     3901 acaaggagag cgcgccgctc gtgcgcatcc tggcgcgcga tggcagcctg ctcgcccagc
     3961 gcggcgggga gggctcctac gtgccgatcg attttttgcc tccccatctc atcgacgccg
     4021 tcgttgccac ggaagatcgc cgcttcttcg agcattgggg tgtcgatctg gcgggcgtgg
     4081 cacgcgcact cattaccaat ttgcgcctcg gccgcctggt ccaggggggc tcgacgattt
     4141 cccagcaact ggccaagaac ctgtttctcg gccccgagcg caccttgacg cgcaaaatcg
     4201 gcgagctcgt gctggcgctt tggctcgagt tccgcctcgg caagcgcgac attctcgagc
     4261 tctatctcaa tcgggtctat ttcggcgctg gcgcctgggg catcgatgcg gccgctcacc
     4321 gttttttcga taaatccgcg caggctctga gcctggccga atcggcagtg ctggcgggcc
     4381 tgctcaaggc cccctcgcgg ttctcgcccg ccagcaatcc agtgctcgcg cgcgcccgcg
     4441 cgcgcacggt gttgagcaaa atggtggagg cagggcggct cgacccggta cgcggcgagc
     4501 aggtggcgcg cgcgcccttc cgttttgccg ccttggcgcg gcagcggcag gccggcgccg
     4561 actatgccgt cgacgccgcg ctcgagcgcc tgccgacgct gatcgcgtcc gcgggcgagg
     4621 cggacctgat cgtcgaaacc agcatcgatg cagggttgca acgcaagagc caagccctcg
     4681 ttgccgagct catgcggggg gaagcgcgcg cgctcgaggc cagccaggcg gctttggtgg
     4741 tgttggacct cgacggcggt atccgcacgc tggtcggcgg gcgcgactac gccgagagcc
     4801 agttcaatcg tgcgctcaag gccaagcggc aaccgggctc ggccttcaag accttcgtct
     4861 acctggcagc gctcgagagc gggctgacgc ccgacagcat cgtgcaggac ctgccgctgt
     4921 tgggcacggg ctggagcccg cgcaacgaag gggccggcta tcgtggcgct gttcccctgc
     4981 gcgaggcgtt ggcccagtcc atgaatgccg ccgcggtgcg cctgcacctg agcctcaccc
     5041 cgtcgcggac gctcaccgcc gcccgccgcc tcggcattcg ctccgagctg cgccaagacg
     5101 cttctctggc gctcgggact tcggaggtta gtttgctcga gctgaccggc gcctatgggg
     5161 tcattgccca tggcggccgc gcgcttgagc cctacctcat ccggcgcgtg cgcaccgccg
     5221 cgggcgaggt cttgttcgaa cggcgcgccg agccgagcac gacgctgatc gccccgaaca
     5281 tagccggcgc tttgagcgac atgttgctgg gcgtcgtcag ccggggcacg ggccggcgcg
     5341 ccgctctcgc cgaacacgcg gtggctggca aaaccggcac cacgcaggat ttcaaggacg
     5401 cttggttcgt cggctacacg gcgcacctgg tcgcgggggt ttgggtcggc aacgacgatg
     5461 cacgaccgat gcgcaaggtg accggcggca gcctgccggc gcggctatgg cacgacgtca
     5521 tgagcatcgc ccatgagggc ttgccaccgc gggcgttgcc gggcagcccg cccagcgagc
     5581 gcgccgggct tggcccatcc cgcgccctcg acggcgcagg cgccacacga ccggcgctcc
     5641 agcgcggcgt gccggagttt ttcgccggcg cgcgcgccgg cgccgaaccg gcgccggcgg
     5701 tcaccgctca aagtgtggtg cgctgaaact tgaggggcgg cgtgacgggc gggcccagct
     5761 cgtcggcgcc gtagcgatgc agatcggcgc catgatggcg caaccagcgt cgtgccgcct
     5821 cgagctccgg caccagacgc gccaccaagg tccaaaactt gggtccgtgg ttcatctcgc
     5881 gcaaatgcgc gacctcgtgc gcggcgacgt agtcgaggac atgcgccggc gccaaaatca
     5941 agcgccagga aaacgacaac aaaccactgg tggaacatga gccccagcgc gtggtctgat
     6001 cgcgcaggca aagccggcgt acgcgcacgc cgagcgcacg ggcgtgcctg ctcacgcagg
     6061 cgtcaaggtc ggcgcgggcc tcgcccatca gccagtccgt caatcgccgt gcggcgtgct
     6121 cgccgcggcc ggaaacctcc agcgtcgggg tcttgccggg cgcgcgcgag atgcgcacga
     6181 cggccgcacc agggcgcggt ccgagaaagc gcaggcgatg catgcccccg cgcaagggaa
     6241 tcaaggcgcc atcgacgaag ggcaccggtt caggcaccga gcccaagcgt tcgcgtaccc
     6301 agtcgatgtg cgaacgcaag aagcggtcgg cctcttccat gcggcactgc gccggcagcg
     6361 tgacgaccac ccagcgtcgc gtattgctga cgcgcaaggt caatcggcgt gcacccgggt
     6421 gacgccgtac ttcgacgggt gcgctgatgc cgtgtactct cagcgccact gactttcgct
     6481 tgcccaccgg caatgtgaaa ccccctctcg cgaccatcac ccgctcagcg cgcctaaagc
     6541 ctgcgccggc gttgcgcgtc tggcctagca aaataatgcg accagaacag tggcaacaat
     6601 cgtgttgcgc gctgcgctgt ggcgtcgctg cgacagacct gacgagaccg gttgacagcg
     6661 gccgagaagc gtgggttagc gcgccgcgaa gctaaatgcc aaactatcga aatcggcact
     6721 gctgcggcga cggcgcggca tggctgccat gtgctccagc tgggcgatgc tgagaccctc
     6781 gacggagcag gggtcgtgcg cgcgcatgaa tgcgctgatg cgcggagcga tctgagcccg
     6841 gaaccgcgag ccgttgaaga tgccgtaatg cccgacgcgt gggcactcga agtgacgctt
     6901 gtccgcctcc gcgatgcccg tacacaaagc cagcgcggcg tggcactgcc cggtgccggt
     6961 gatatcgtcc ttttcgcctt ccaccgtcat cagcgccggc cgacggatgg cgccgaggcg
     7021 aatgggtcgg ccgcggtgcg tcatcaaccc gcgcggcagc aggtgcttga cgaacaccgt
     7081 atcgaccgtt tgcagataaa actcggccgt gaggtccatg accgccagat actcgtcata
     7141 aaattcgcgg tgcttttccg ccgagtcgcc gtccccgcgc acgaggtgga ggaacatctc
     7201 cttttgcgcc tcgaggtggc ggtcgagatt catggtcatg aagccggaga gctgcaggaa
     7261 gcccgggtag acgagacgac catggcccgg gtttggccag ggcacgttgg tgatgaccgt
     7321 gcggcggaac cagtcggtgc cgcggcgctc cgcgagctcg ttgaccacgg tctgattgat
     7381 gcgcgtgtcg atcggaccgc cggccagaac gagggagagg gggacgtcgg cctccgcgtc
     7441 ctcctccatc agtgccgtcg cggccagcac cggcacggcc ggctgacaca cggcgaagac
     7501 gtgcacatcg cccttgagga agcggaagat ggcgatcatc gcatcgatgt aattgtcgag
     7561 atcgaagctg cccgccgcct gcgccaccat gcgcgcgtcc tgccaatcgg tgatgtagac
     7621 gtcgtggtcg ggcaggaagg tttcgaccgt gccgcgcagc agcgtggcga aatggccgga
     7681 catgggcgcc accagcagca agcgcgggtc gcgcgccccc tcgctgacat cgatgtcgcg
     7741 gcggaaccgc accaagcggc agaacggcgt gcgccagacc tcctcctcct cgaccttgac
     7801 caggcggccg cgcacctggg cttgggtgat gccgaaggcg ggcttggtgt agcgccgcgt
     7861 ggcccgctcg aacacctcgc aggcagcaac ggcgctgcgc ccgacggggg tatgggtgag
     7921 cggattgagc gggttgaaga acagccggca agagtcggcg gcagctcggg ccggccgaca
     7981 cgcggctcga ccgagctcat accagtgata gagcacgcag ttcccccggt tttgcagtcc
     8041 ctgcgccaac cacgctatgc agcggcttca tgcggctgat gccgtcttca cgtccgtctc
     8101 ggcgactttg gcatggcgca caaagaactg tgcactgatt cgaaagttcc taaaagactt
     8161 gtgacttcaa tgcaacagct tcgcgaggcg ctcggccatg ctgtcggcgt tgctcgcgtg
     8221 cgtgaagtgg gcgaggtagg ccccatccgg tcccatcaga tagatgatgg cggaatgatc
     8281 gaacgtgtag cccgcactcg acttgggatc cggcaccttt tttacgtaga cgcggtaggc
     8341 cttggtgacc gcctcgatct cggccggcgt gccggagagc ccgaccaaac gagggtgaaa
     8401 gcttttcacg taggtggcga gctgggtcgg cgtgtcgcgt tccggatcga cgctgatgaa
     8461 cagcggggtg attttctgcc ccttggggcc cagcttgtcg agcgccgccg ccatcacctg
     8521 cagcgccgag ggacacacgt cgggacagtt ggtgaaacca aagaacacca acagatagcg
     8581 gccgcggaag tcgttgtcgg tgacgcgttt gccggtctga tcggtgaggc taaacggacc
     8641 gccaaccagg gcgtgcccga ccgagggcgc gccggcaggc aagaggcgct cgcgcgctgc
     8701 ggggaagatt gccagcgccg ccgccgcacc caccagcaat cccgctacaa cgatgagcgc
     8761 gagcctcagg ctcgagttgg tcacggctct gctacctcat tcgcccatct gcaccagcgt
     8821 tccgtgcctg cgcgcacttt gcaggaagta gccaaacgct gcaaacccga gcgccaaata
     8881 gatgacgttc aggacgaacg ccatcagcat ggaggctgcc acgaaccttc cctccagcac
     8941 aatcgcgcgc aggccttcaa acacgtgggt cggcggcagt gccagggcga cgccttgcag
     9001 ccagccgggc agggtgtcga cgggataata gacgcaacag agcggcaaga acacaaacac
     9061 gatcagccag gcgaagctct cggccccaag cccccagcgc agaaccacgc cggtggaaac
     9121 gagacccaaa gcccagctcg tcaacaccag gttggcgaag aatgcggcga acgccgagcc
     9181 gagcgacaac acgttaaagc cgaagaaggt ataggccagg atggccaccg gcaccatggc
     9241 caccatcagc ttgatgaagc tgaccgccat caaggcggca acgagctcgc ccgggcgcag
     9301 cgggctcagc atcagatggc cgagattgcg cgaccagatc tcctcgagga aggccaccgc
     9361 aaagccgagc tggctgcgca cgagaatatc ccacagcaac acggcgccga tgaagagccc
     9421 cgccgccttg gcggcaaaac tcgaggtgct cgccagatag ctctgcagaa agccccacat
     9481 cagcatctgc acgagcggcc agtagatcag ctcggcggtg cgcggccagg aggagcggat
     9541 cacgtaccag tagcgcagga tcatccccca gatccgaccg gcggaggcgg cgagcgtcgt
     9601 ctgctcgttg agggccggcg cctgccagcc aggttcgctg tcgccggtca tgccgcgctc
     9661 tcctcgttag cgcggctgcg ccgggcaatg gcgagaaata cctcctcgag cgtgctgcgc
     9721 ccgaagcgct cgataagttg cgcgggggtc tcatcggcga tcacgcggcc tttttccagc
     9781 atgatcacgc gatcggccaa gcgctcgacc tcggccatgt tatgcgacgc caagacgacg
     9841 gtggcccggc gtgcacgtaa gtagtgctgc agcttggcgc gcacccagtc ggccgtatcg
     9901 ggatcgagcg aggcggttgg ctcgtcgagc agcaacagct ccggctcgtt cagcagcgcc
     9961 ttggcgagac tgacgcgcgt cttctgcccg gctgacagtt tgccgtaggg acggtcgaga
    10021 aactcgctga gccgaagatc tgccgcaatc gccgccactt gcgacttgag ttgacgcacg
    10081 ccgtagagac ggccatagac ctccaggttt tggcgcacgg tcaggcgcat cggcacatcg
    10141 acgtagggac tttcgaaatt catccggtgc agcacgcggt aacgctcacg cgccatgtcg
    10201 gcgccgaaca cgcgcgcctg cccggaggtc gggatgacga ggcccatcag catggaaatg
    10261 gtggtggtct tgccagcgcc attgccgcca agcagcgccg tgacggcccc cgcggtcagc
    10321 ttgaagtcga ggccatcgac ggcggcgacg tcgcgataga ccttacgcag gcgcaccacc
    10381 tcgacggggg gcggctcggc gccaggcggg ggagggatcg gcctctgcat gcgctcgtcc
    10441 aagggggttg gccaagctgc agctaaagat cagcccgcgg cacctggcgg tatggctggt
    10501 aaccttgccc tatctggggt agcaaggccg ccgacgcaag cgcaacgcca ggcggggccg
    10561 gtcggctccg ggcggcgcac gaacgaaacc atcgtcctat gctgcggcgg cgtgaaagac
    10621 cctgggacga aacggccggt agccggctgc gcttgcagac ggccgtccgg ctgcgctggt
    10681 tcggcgttat cggccagctc ctgacggtgg gtgtggtcta tctgctgctg ggctttcagc
    10741 tcccgctcgg catctgtctc gcctttatcg ccttgtccgc ctggctcaac gttttcctgc
    10801 gcatccgctt tcccgcccgc acgcgcctca gtacgggctt cgccaccagc ctgcttgcct
    10861 acgacatcct gcagcttgcc gccctgctgt ttttgaccgg cggggcggac aatcccttta
    10921 ttttcttgct ggtcgccccg gtgacggtat cggccgccac gctgccaccg cgcaacacca
    10981 tcgcgctcgg cctgttgggc gccggcatgg cggtgctgct gctgttctac tcgatgccgc
    11041 tgccctggta tcccggcagc cgtcccgacc ttcccatgct ctatagcgtc ggggtgctgg
    11101 cttcggtttt gtccggcatg gtgtttcttg cgctttacgc ctggcggctc gccaaggagg
    11161 cacgccagat gtcggatgca ctggcggcca ccgagctggt gctggcgcgc gaacagcagc
    11221 tgcacgccct tgacggattg gccgccgccg ccgcccatga gctcggcacg ccgctctcca
    11281 ccattgccgt catcgccaaa gagctggcgc gcgaaccggc cgccggcggc caattcgccg
    11341 acgacatcgc gctgctgcag gcgcaagcca tgcgctgccg ggagatcttg accaagctga
    11401 cgcgcagtcc cagcgagccc gatccgctgc acgcacgcat gagcgttaca cagctgatcg
    11461 acgaagccgc ggaaccctat cgcagcacca gctcactgag cattagtgcg gagccggaag
    11521 cgggtgtcga cggcgaggcc gccctggagc ccgtgggcga gcgcaagccg ggcgtcatct
    11581 acggcctgag caatctgatc gagaacgcgg tcgacttcgc acgtcagcgc gtcgaaatcg
    11641 tcgccaggtg gtcggcgcgt gacgtgctgc tgaccatcag cgacgacggt cccggcatcc
    11701 ccctcgatgt gctcgacgcc ctcggcgaac cctacatcac cacgcgcggc agccggcgcg
    11761 ccggccagat caagaacggc gaaccggccg ggctaggcct tggctttttt agtgccaaga
    11821 ccctgctgga acgctcg
//
LOCUS       DASTMT010000377         1904 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_6013158, whole genome shotgun sequence.
ACCESSION   DASTMT010000377 DASTMT010000000
VERSION     DASTMT010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 1904)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1904)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1904
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6013158"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..758)
                     /locus_tag="VFR00_08865"
     CDS             complement(<1..758)
                     /locus_tag="VFR00_08865"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010659878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HEU0159413.1"
                     /translation="MSQPLKLGVAGLGTVGAGLVQLFEEHGARLALALGREITIAGVS
                     ARSRDKPRGIKLANIPWFDAAERLATDPAIDVFVELIGGEEGAARAAVEAALKAGKHV
                     VTANKALLAKHGTELARLAEAHAVALNFEAAVAGGIPVIKTLREALAGNQIRRVYGIL
                     NGTCNYILTQMQEEHRAFAEVLKEAQDKGYAEADPTFDIGGFDTAHKLALLTSLAFGT
                     KVALDQIHVEGIKGISQADIEAADVKGRIGLGIAL"
     gene            complement(770..>1904)
                     /locus_tag="VFR00_08870"
     CDS             complement(770..>1904)
                     /locus_tag="VFR00_08870"
                     /EC_number="2.6.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013166179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HEU0159414.1"
                     /translation="NPDMPTPQHIVEKLVETVAKPRTNRYSASKGIPGLRKAQAAYYA
                     RRFGVKLNPETQVVATLGSKEGFANMAQAISAPGDVILCPSPTYPIHEFGFIISGAAI
                     RHIASNGGGLAAADPHTEFLRGVERAVKHSIPRPLAVVLSYPSNPTASTADLAFYTEA
                     VQLAKKLELIILSDLAYAEIYYDNNPPPSVLQVPGAMDVTVEFTSLSKTYAMPGWRMG
                     FAVGNERLIAALARVKSYLDYGAFTPVQVAAAAALNGPQECVDEIRKTYRARRDCLVE
                     SFARAGWPIPAPAASMFAWAPIPDAYRQIGSVEFAKMLIDKADVAVSPGLGFGEYGEG
                     YVRIALVENEHRIRQAARNIKKFLARAPETMHNVIPIPQKASR"
BASE COUNT          319 a          588 c          649 g          348 t
ORIGIN      
        1 agggctatgc cgaggccgat ccgacctttg acatcggcgg cttcgatgtc ggcctggctg
       61 atacccttga tgccctcaac gtggatctga tcgagcgcca ccttggtgcc gaacgccagg
      121 ctggtcaaaa gcgccagctt gtgcgcggta tcgaagccac caatgtcaaa ggtgggatcg
      181 gcctcggcgt aacccttgtc ctgggcctcc ttcagcacct cggcgaaggc gcggtgctcc
      241 tcctgcatct gggtcaagat gtagttgcag gtgccgttga gaatgccgta gacgcggcgg
      301 atctgattgc cggccagggc ctcgcgcaag gtcttgatga ccgggatacc accggccacc
      361 gcggcctcga agttgagggc gacggcgtgc gcttcggcca gccgcgccag ctcggtgcca
      421 tgcttggcca acagcgcctt gttggccgtc accacgtgct tgccggcttt caaggcggcc
      481 tcgacggcgg ctcgcgccgc gccctcctcg ccgccgatca gttcgacgaa gacgtcgatc
      541 gccggatcgg ttgccaagcg ttcggcggca tcgaaccagg gaatgtttgc gagcttgatg
      601 ccgcgcggct tgtcgcgcga gcgggcgctg accccggcaa tcgtgatctc gcgacccagc
      661 gccaacgcca ggcgggcgcc gtgctcctcg aagagctgca ccaatccggc gcctacggtg
      721 ccgaggccgg cgacgccaag cttcaacggt tggctcatgc gaaccaatac taacgcgatg
      781 ccttttgcgg aatagggatc acgttatgca tggtttcggg cgcgcgcgcg agaaactttt
      841 tgatgtttcg cgccgcctgg cggattcggt gctcgttctc gaccagggcg atgcgtacat
      901 agccctcgcc gtactcgcca aaacccagcc ccggcgatac ggccacgtcg gccttgtcga
      961 tcagcatctt ggcaaattcc accgagccga tctggcggta ggcatccgga atgggcgccc
     1021 aggcgaacat ggaggcggcc ggggcgggga tgggccagcc ggcacgggca aagctttcca
     1081 ccaggcagtc gcggcgtgcc cgataggtct tgcggatctc gtcgacgcat tcctgcgggc
     1141 cgttgagagc ggccgctgcc gccacctgca cgggggtaaa ggcgccgtag tcgagatagg
     1201 atttgacacg ggcgagggcg gcgatcaggc gctcgttgcc gacggcaaag cccatgcgcc
     1261 atcccggcat ggcataggtt ttcgacagcg acgtgaactc caccgtcacg tccatggcac
     1321 cgggtacctg cagcaccgac ggtggtgggt tgttgtcgta gtagatttcc gcataggcga
     1381 ggtcggagag aatgatcagc tcgagttttt tggcgagctg caccgcctcc gtatagaagg
     1441 caaggtcggc ggtcgacgcc gtcgggttgg acggatagct caagaccacg gcgagcgggc
     1501 gcggaattga atgcttcacc gcgcgctcaa cgccgcgcag aaactctgtg tgcgggtccg
     1561 ctgccgccag gccgccgccg ttcgaggcga tgtggcggat ggcggcgccg gaaatgatga
     1621 agccgaactc gtggatcggg taggtcgggc tcggacacaa gatgacatcg ccgggcgcgc
     1681 tgatggcctg cgccatgttg gcgaagcctt ccttggatcc caaggtcgcc accacctgcg
     1741 tttcgggatt gagtttgacg ccgaagcggc gcgcatagta ggcggcctgc gccttgcgca
     1801 gacccggaat gcccttggag gccgagtagc ggttggtgcg cggcttggcg accgtctcca
     1861 ccagcttctc gacgatgtgc tggggcgtgg gcatgtccgg gttg
//
LOCUS       DASTMT010000378         6101 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_1195272, whole genome shotgun sequence.
ACCESSION   DASTMT010000378 DASTMT010000000
VERSION     DASTMT010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 6101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6101
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1195272"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1421
                     /gene="ligA"
                     /locus_tag="VFR00_08875"
     CDS             <1..1421
                     /gene="ligA"
                     /locus_tag="VFR00_08875"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019198406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEU0159415.1"
                     /translation="FAYGWGAASTLPAATQAGVYAAFQRWGLPLNPLFRVCASTAELI
                     AFHRTMAEKRATLGYDIDGVVYKVNRLDWQQRLGFVSRAPRWAIAHKFPAEEVMTRLN
                     DIEIQVGRTGALTPAAKLEPVTVGGVVVSNATLHNEDEIARKDIRIGDTVIVRRAGDV
                     IPQVLAVVLEKRPKAAKPYKMPQLCPACGSHAVREVDERTGRIDVVRRCTGGLICPAQ
                     AKERLKHFVSRNALDIEGLGEKQIELFYDEGRIMQPADIFTLAAREAHLPAGKRLADK
                     KGFGAKSIDNLFKAIAARRRVPLDRLIYALGIRHIGETTARDLAKALRTLEAFRAAAS
                     AAAAGGKASAAYQDLDAIEGIGTTVIDALIDFFGEPHNLAALADLLAEIEVEPFARAS
                     TRATPVTDKTVVFTGTLERMTRSEAKALAERLGAKVTGSVSAKTDYVVAGADAGSKLA
                     KARELGVSVLSEDDWLKLAAGE"
     gene            complement(1449..2219)
                     /locus_tag="VFR00_08880"
     CDS             complement(1449..2219)
                     /locus_tag="VFR00_08880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0159416.1"
                     /translation="MRNFRSLDAHKVIETIAALEQRIGERFPSAGLKAVCGELLTLAR
                     ESSQRAQAIARTNTALRVLVIVFLLGSFWVLLWIASLIDFTRTSADNVYSVLQGIEAA
                     ANLAVLMGAAVFFLVRIEERVKRRRALSALHELRSIVHVIDMHQLTKDPSGSVSIGGS
                     TASSPARLLSRFELSRYLDYCSEMLSLTSKVAVLYAQSFPDTEVTDAVSDIERIAAGL
                     SQKIWQKIMILQAAGIDTGKAAAPTPPAAALVRPHPAR"
     gene            complement(2239..3081)
                     /gene="dapD"
                     /locus_tag="VFR00_08885"
     CDS             complement(2239..3081)
                     /gene="dapD"
                     /locus_tag="VFR00_08885"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006698917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HEU0159417.1"
                     /translation="MSNTDLKSIVEKAWDARETISLATRGEVREAVETALELLDRGEA
                     RVASKQDGSWVVNQWLKKAVLLSFRLYDMVSIAGAPGGASWWDKVPSKFAGMGPEAFR
                     AAGFRAVPGSIVRRSAYVAPGVILMPSFVNLGAYVDSGSMVDTWATVGSCAQIGKNVH
                     LSGGVGIGGVLEPLQANPTIIEDGCFIGARSEVVEGVIVGEGSVLAMGTFIGASTKVV
                     DRASGKVHVGHVPPYSVVVSGSMPGKPYPNNEPSPSLYCAVIVKTVDAQTRAKTSINE
                     LLRD"
     gene            3227..4063
                     /locus_tag="VFR00_08890"
     CDS             3227..4063
                     /locus_tag="VFR00_08890"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HEU0159418.1"
                     /translation="MTIRFLPWATIASVLACAPVTATAQELAMAPAMAQPSFPTAPPV
                     LSPECRSRRIVGEMFRRPLKALSRAVRANNTVKVLAIGSSSTVGVGASSPAASYVANL
                     ETTLEGSFTGMNFDVVGRGMSGEVAQGAADRMKREVEETKPDLVVWQVGTNDAIRHVS
                     IDSFKACLKKALAWLAENRIDVVLIDPQYGAALTKDEYYEQVVAAIAEVAREQRVLLV
                     DRFEAMRELQKDRGDTFYLSADNLHLNDRGHRCMAEQLARAIVGGLLQADAESEQPVF
                     YP"
     gene            complement(4086..4757)
                     /locus_tag="VFR00_08895"
     CDS             complement(4086..4757)
                     /locus_tag="VFR00_08895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012110510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEU0159419.1"
                     /translation="MTERERLGSKLPARTAALAGLVAASVGFAAIYVTLGHSDNGARA
                     PTGATVAAQGQGGAAASAAEEGASAAGRSELPAFVFRKEPEALPEIKFQDAEGRERTL
                     ADWRGKVVLLNLWATWCLPCRKEMPALDRLQATLGSDQFQVVAISVDRTGLPGAKKFL
                     EETKTPNLAVYADPTARLASSLRAAGLPATLLIDKEGRELGRLLGPAEWDGEAAQRLI
                     QSALR"
     gene            4829..>6101
                     /gene="argH"
                     /locus_tag="VFR00_08900"
     CDS             4829..>6101
                     /gene="argH"
                     /locus_tag="VFR00_08900"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018388201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEU0159420.1"
                     /translation="MWGGRFEMSAAEVLEDINTSIDFDKALAPFDVRASKVHAAMLAA
                     QGIISKEDGRKIAAGLDRVLAEIEAGEFVFSRELEDIHMHVEARLHELIGPAAGRLHT
                     ARSRNDQVATDFRLYLRDFIDGADAALNALQLALARKADEYAELAMPGFTHLQPAQPV
                     TFGHHLLAYVEMLSRDRGRFADARARLNECPLGAAALAGTSFPIDRHRTAEALGFDRP
                     TANSLDAVADRDFVLETLAAAAITAVHLSRFAEEIVLWMTPQFGFVRLSDRFTTGSSI
                     MPQKRNPDAAELVRAKVGRIAGAFQSLLMVIKGLPLAYAKDMQEDKEVTFDALKALRL
                     SLFALTGMLEDLKPNAAAMAAAAQAGFSTATDLADWLVANLRLPFREAHHLTGRLVAA
                     AESSGVSLDKLPLEVMQEMHPGITQAIFSVLS"
BASE COUNT         1044 a         2005 c         1980 g         1072 t
ORIGIN      
        1 ttttcgccta cgggtggggc gcggcaagca cgctgcccgc cgcgacacag gctggcgttt
       61 acgcagcctt ccagcgttgg gggctgccgc tcaatccgct ctttcgggtg tgcgcgagca
      121 ccgcggagct gatcgccttt catcgcacta tggccgagaa acgcgccacc ctcggctatg
      181 atatcgatgg cgtggtttac aaggtgaacc ggctcgactg gcagcagcgg ctcggcttcg
      241 tctcgcgggc gccgcgctgg gcgattgcgc acaagtttcc cgccgaagag gtcatgacgc
      301 ggctcaacga catcgagatt caagtcggcc gcaccggcgc cctgaccccc gccgccaagc
      361 tcgagcccgt gacggttggt ggcgtcgtcg tttccaacgc caccttgcac aacgaggatg
      421 agattgcgcg caaggacatt cgcatcggcg ataccgtgat cgtgcgccgc gccggcgacg
      481 tgatcccgca ggtgctcgct gtcgtgctcg agaagcgccc gaaggctgcc aagccctaca
      541 agatgcccca gctctgccca gcctgtggca gccacgcggt gcgcgaggtg gacgagcgta
      601 ccggtcgcat cgacgtcgtg cgccgctgca ccggcggctt gatttgtccc gcgcaggcca
      661 aagagcgcct caagcacttc gtctcgcgca atgccctcga catcgagggt ctgggcgaaa
      721 agcagatcga gctgttctac gatgagggcc gcattatgca accggccgac atcttcactt
      781 tggcggcgcg cgaagcgcat ctgccggcag gcaaacgtct tgccgacaag aagggctttg
      841 gcgccaaatc gatcgacaac ctgttcaagg ccattgcggc gcgtcgacgc gtgccgctcg
      901 atcgcttgat ctacgcgctc ggcatccggc acatcggtga aacaaccgcg cgcgatctcg
      961 ccaaagcgct gcgcaccttg gaggcctttc gtgccgccgc cagcgccgcg gccgcgggcg
     1021 gcaaggcgag cgctgcctat caagacctcg acgccatcga ggggatcggc accaccgtta
     1081 tcgacgcact gatcgatttt tttggcgagc cgcacaacct cgctgcgctc gctgacctgc
     1141 tggccgagat cgaggtcgag ccgttcgccc gcgcctcgac tcgcgccacc cccgtcaccg
     1201 acaagacggt ggtgtttacg ggcacgctcg agcggatgac gcgcagcgaa gccaaagcct
     1261 tggcggagcg gctcggcgcc aaagtcacgg gctcggtctc ggccaagacg gattacgtcg
     1321 tcgccggcgc cgacgccggc tccaagctcg ccaaggcgcg tgagctgggg gtttccgtct
     1381 tgagcgagga cgattggctg aagctcgccg ccggcgaata ggcgcccttg ccaagcaaaa
     1441 tcccccgctc agcgcgcggg gtgcggccgc accagcgccg cggccggcgg cgtgggagct
     1501 gccgccttgc cggtgtcgat gccggcggcc tgcaggatca tgatcttctg ccagatcttc
     1561 tgcgacagtc cggccgcaat gcgctcgata tcgctgaccg catcggtcac ctccgtgtcc
     1621 ggaaaacttt gtgcgtagag cacggcgacc ttagaggtga gcgagagcat ctccgaacag
     1681 tagtcaagat agcggctgag ttcgaatcgg ctgagcaggc gcgccggcga ggacgccgtg
     1741 ctaccgccga tgctgaccga gccgctggga tccttggtga gctgatgcat atcgatgacg
     1801 tgcacgatcg agcgcagttc atgcaaggcg gacagcgccc ggcgccgttt gacgcgctct
     1861 tcgatgcgca ccaggaagaa aacggccgcg cccatcagca ccgccagatt ggccgccgcc
     1921 tcgatacctt gcagaacgct atagacgttg tcggcgctgg tgcgggtgaa gtcgattaac
     1981 gaggcgatcc aaagcaaaac ccagaagctg cccaacaaaa atacaataac cagcactcgc
     2041 agcgccgtgt tggtcctggc gatggcctgg gcccgctgcg aactttcgcg cgccagggtt
     2101 agcagctcgc cgcaaacggc cttgagcccg gcgctgggaa agcgctcgcc gatgcgctgt
     2161 tccagggcgg caatcgtttc gatcaccttg tgagcgtcga gggagcggaa gttgcgcacg
     2221 gggcgagctc cggcggcctt aatcgcgcaa caactcgttg atgctggtct tggcgcgggt
     2281 ctgtgcgtcc acggtcttga cgatcacggc gcagtaaagg ctggggctcg gctcgttgtt
     2341 ggggtagggc ttgcccggca tgctgcccga caccaccacc gaatagggtg ggacgtggcc
     2401 gacgtgcact ttgccgctgg cgcggtccac gaccttggtg gaggcgccaa tgaaagtccc
     2461 catcgccagc accgaaccct cgccgacaat cacaccctcc accacctccg accgcgcgcc
     2521 gatgaagcaa ccatcctcga tgatcgtggg attggcctgc aggggctcca atacgccgcc
     2581 gatgccgaca ccgcccgaca ggtgcacatt cttgccgatc tgtgcgcacg aacccaccgt
     2641 cgcccaggta tcgaccatgc tgccgctatc cacataggcg ccgagattga caaaggaggg
     2701 catcaggatg acgccgggcg cgacgtaggc ggagcgtcgc acgatcgaac ccggtacggc
     2761 gcgaaagccg gcggctcgga aagcctccgg gcccatgccg gcgaacttgg agggtacttt
     2821 gtcccaccag ctggcaccgc ccggtgctcc ggcgatgctc accatgtcgt agagccggaa
     2881 cgagagcagc acggccttct taagccattg atttaccacc cagctcccgt cctgcttgct
     2941 ggccacgcgc gcctcgccgc gatcgagcag ctcgagggcg gtttcgaccg cctcgcgcac
     3001 ctctccgcgc gtggcgaggc tgatcgtctc gcgcgcgtcc caggccttct cgacgatgga
     3061 tttgagatcg gtgtttgaca tggttagcgt tctggcgcgg agcgcccgct cctgtcaatg
     3121 gactaggcgc gcggtcgcaa atgttgagag ctgtacacag gctgctttac cagcgcgcag
     3181 tcctggccta gattttgggc atgagaaggc tgcgggctcg ggcttcatga caatccgttt
     3241 tctgccgtgg gcgacaatcg cgagcgtatt ggcgtgcgcg cccgtgacgg ccaccgctca
     3301 agagttggcc atggcgccgg ccatggcgca gccgagcttt ccgacggcgc caccggtgtt
     3361 atcgccggag tgtcggtcca ggcgcatcgt cggggaaatg tttcgtcggc ccttgaaggc
     3421 gctgagccgc gcggtgcgcg ccaacaacac cgtcaaggtg ctggcgatcg gctcttcctc
     3481 caccgtcggc gttggcgcgt cctcgccggc cgcgagctac gttgccaatc tggaaacgac
     3541 gctcgagggc tcttttacgg gtatgaattt cgacgtcgtt gggcgcggca tgtcgggcga
     3601 ggtcgcccaa ggcgcggcgg atcggatgaa acgcgaggtc gaggagacga agcccgatct
     3661 cgtggtctgg caggtcggca ccaacgatgc cattcgccat gtcagtattg acagtttcaa
     3721 ggcctgcctg aaaaaggcgc tggcctggct cgcggaaaac cgcatcgatg tggtgctgat
     3781 cgatccgcag tacggcgccg ccttgaccaa ggacgaatac tacgagcaag tggtggctgc
     3841 gattgccgag gtggcgcgcg aacagcgcgt gcttttggtc gatcgcttcg aggccatgcg
     3901 cgagctgcag aaggatcgcg gcgacacctt ctatctctcc gccgacaatc tgcacctcaa
     3961 tgatcggggc catcgctgca tggccgagca gctggcgcgc gccatcgtcg gcggcctctt
     4021 gcaggcggac gccgaaagcg agcaaccggt gttctatccc tgatcctgtc gggttcggtg
     4081 ctaacctagc gaagggcgga ttggatgagg cgctgcgccg cctcgccatc ccattccgcc
     4141 ggtcctaaca gtcgcccgag ctcgcggccc tccttatcga tcaataaggt ggcgggcagc
     4201 ccggcggcgc gcagggagga ggccaagcgc gcggtcgggt cggcataaac ggcaaggttg
     4261 ggggtcttgg tctcctccag aaacttcttg gctcctggca gccccgtgcg atcgacgctg
     4321 atcgccacga cctggaactg gtcggagccc agtgtggcct gcagccggtc gagcgctggc
     4381 atctccttgc gacagggcaa acaccaggtc gcccagaggt tgagcagcac caccttgccg
     4441 cgccagtcgg caagcgtgcg ctcccggccc tccgcatcct ggaatttgat ctcgggcagg
     4501 gcttcgggct ccttgcgaaa cacgaaggcg ggcagctcgc tgcgacccgc cgcactggcg
     4561 ccctcctcgg cggcactcgc cgccgcaccg ccttggcctt gcgccgccac ggtggcacct
     4621 gtgggtgccc gcgcgccgtt gtcagaatgg ccgagcgtca cgtatatggc tgcgaaaccg
     4681 acgcttgccg ccaccagccc ggccagcgcg gcggtgcgcg ccggcagctt ggagccaagc
     4741 cgctctcgct ctgtcatagt ttgaggctcc taaatgccgc tccagcttaa gcaggacatg
     4801 gcccgtgacc gataaacccg ccaataagat gtggggtggc cgctttgaga tgtcagcggc
     4861 cgaggtgctg gaggacataa acacctccat cgacttcgac aaggcgttgg ctcccttcga
     4921 cgtgcgtgcc tccaaggtgc acgcggccat gctggccgcc cagggcatca tcagcaagga
     4981 ggacggacgc aaaattgcgg ccggccttga ccgcgtgctg gccgagatcg aagcgggcga
     5041 gttcgtgttt agccgcgagc tggaagacat tcacatgcat gtggaggcgc gcctgcacga
     5101 gctgatcggc cccgcggcgg ggcggttgca tacggcgcgc tcgcgcaacg atcaggtggc
     5161 gaccgatttc cggctctatc tgcgcgactt tatcgacggc gccgacgctg cgctcaacgc
     5221 cctgcagctt gccctggccc gcaaggctga cgagtacgcc gagctggcca tgcccggctt
     5281 cacgcatttg cagccggcgc agcccgtgac ctttggtcac catctgctcg cctatgtcga
     5341 aatgctgagc cgggatcgcg gccgcttcgc cgacgcgcgt gcccggctca acgaatgccc
     5401 gctcggggcc gctgcgctgg ccggcacctc gtttcccatc gaccggcatc gcaccgccga
     5461 ggcgctcggt ttcgatcggc cgacggccaa ctcgctcgat gccgtggccg accgcgactt
     5521 cgtgctggaa acgctggccg ccgcggccat cacggccgtg cacctgtccc gtttcgccga
     5581 agagatcgtg ctctggatga cgccacaatt cggcttcgtg cgcctgtcgg accgctttac
     5641 caccggttcc tcgatcatgc cgcaaaagcg caaccccgac gcggccgagc tcgttcgcgc
     5701 caaggtaggg cgcattgccg gggcttttca gtccctgctg atggtcatca agggtctgcc
     5761 gctcgcctat gccaaggaca tgcaggaaga caaggaagtg acgttcgacg ctctcaaggc
     5821 cttgcggctg tcgctgtttg cgctgacggg catgctggag gatctaaagc ccaatgcggc
     5881 ggccatggcg gcggcggccc aagcgggatt ttctacggca accgatctgg cggattggct
     5941 ggtcgcgaac ctgaggctgc cctttcgcga ggcgcaccac ctgacggggc gcctggtggc
     6001 ggccgccgag tcgagcggtg tgagcctcga caagctgccc ctcgaagtta tgcaggagat
     6061 gcaccccggt attacccagg ccatcttttc cgtgctgtcc g
//
LOCUS       DASTMT010000379         1584 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_3489931, whole genome shotgun sequence.
ACCESSION   DASTMT010000379 DASTMT010000000
VERSION     DASTMT010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 1584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1584
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3489931"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..191)
                     /locus_tag="VFR00_08905"
     CDS             complement(<1..191)
                     /locus_tag="VFR00_08905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0159421.1"
                     /translation="MQKRATYRQLAKFNRRIAEGRDRVALERARVVERSRRDGGDPEG
                     LLSVLERSLQAKIQYRAML"
     gene            563..925
                     /locus_tag="VFR00_08910"
     CDS             563..925
                     /locus_tag="VFR00_08910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0159422.1"
                     /translation="MGERGEAAGRYAAGGEQVIERTLGGDELCAGRDGLALHRFIKVM
                     DAVFLVGAELQTRGELKHVQRTWLAVEFRAKRQAHAAPSLQIGELPLAQGLDVACVET
                     GISRVFMLMLGRGGPSEG"
BASE COUNT          251 a          503 c          531 g          299 t
ORIGIN      
        1 atcagcatgg cgcgatactg gatctttgcc tgcaaggagc gctcgagcac acttaacagg
       61 ccttcggggt cgccgccgtc gcgccggctt ctttccacga cccgggcccg ctcaagggcg
      121 acgcgatccc gtccttcggc aatgcgtcgg ttaaactttg caagctgacg gtaggtggca
      181 cgtttttgca tggggacccc gcccggcaat ttgaggcgtg gtcctagcat cgcgcccatc
      241 tccgctcatc ggaccaaggt cgctaccaat tcgaccctcc ggctgcagcc taatctttgc
      301 cgtaaccaat ttgaaccgag ctttggggcc taaatccgcg gcgctttcgc ggcgaaaaag
      361 cccttctcgg aggcccgatg gcggcggcat cgccacaccg tcggcgcagc cggctgcgct
      421 tggttaaggg caccgtcaac gtggcgagcg atagccgcgc agctcggcgt atcgccgcct
      481 ttgctcgggg gtgagcaccg gcgccgttgt cagatggtag tgctaatgaa cggcgcggag
      541 ctcgccctgc cgcgccgatt tcttgggtga gcgaggcgag gctgccgggc gctatgctgc
      601 cggcggcgaa caggtgatcg agcgcacgct cggcggcgat gagctttgcg ccggccgcga
      661 tggcttggct cttcatcgct tcataaaggt gatggacgcg gtgtttctgg tcggggctga
      721 gctgcagacc cgcggcgagc tcaagcacgt gcagcggacc tggctagccg ttgagttccg
      781 cgctaagcgc caggcccatg ccgcgcccag cctgcaaatc ggcgagctgc cgctcgctca
      841 gggccttgat gtcgcgtgcg tcgagaccgg cataagccgg gtgttcatgc tgatgttggg
      901 ccggggcggg cccagcgagg gatagcaaaa ctgccgccac gactaagcgc attcgattgg
      961 cttgcgcccc gtttttctcg attgctgaga cgaggacgac agaggccgct gcgtggccgc
     1021 caaccggaat gaacatcggc gtcctcgttg cgcagctcgg gcgttggcaa aaggggcgcg
     1081 caacatccgt gcgggctgtt agggatgttg tcatgcatgg accgtgccat cggtctgtga
     1141 tcgagatcac agccggccga tcctcgcatg cctagcatcg ccaaggctat cggggcgtgg
     1201 gcgcgtgcta tgtctggcgt cggggtgcgt gcaagacggt cgcttccccg cggggaacac
     1261 ccgatagcca cccgaatccc gccgagcagg tgccattgcc cgcgcgtcag gattattgac
     1321 ccgcgaggcc caagcaggct tgggcttttt gtgacgggag gcggtatcca cctgcccgtt
     1381 agcgccccgg gcttgggcgc ctcgacgacg acggcgtcgg aggcttaccg gccgggtgcg
     1441 cctatccgcc cgcccatctg ctgtcgcgtc cggtgtctcg tcgccaaatt cggtggaacg
     1501 tttgcgggcc cgatcggcgc aagcatcaat tcggcgcctc ccgacgacca ggatagtcct
     1561 cgacgccagc tcggaatggc tgcg
//
LOCUS       DASTMT010000380         2133 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_2502172, whole genome shotgun sequence.
ACCESSION   DASTMT010000380 DASTMT010000000
VERSION     DASTMT010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2133
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2502172"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..716
                     /locus_tag="VFR00_08915"
     CDS             <1..716
                     /locus_tag="VFR00_08915"
                     /inference="COORDINATES: protein motif:HMM:NF012739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HEU0159423.1"
                     /translation="ESKVAYWSAQLARQKTLSERGIVASSKFEEVESNATAAEDRVAV
                     MRKRVDRVAAQLGGDPARAEDDHPMVRQKRAERERAALDLAHTVIRAPLGGTAVNVRL
                     QRGEQIKVATPLFALVADTRPWVEANFKETELTHVRKGQTALVVLDIYPDVVWQAEIE
                     SISPATGAEFAVLPPQNASGNWVKVVQRLPVRLRLLPQAGEPPLRAGMTASVRVDTER
                     QSRLSRLLGGWSAFATSRR"
     gene            complement(724..2064)
                     /locus_tag="VFR00_08920"
     CDS             complement(724..2064)
                     /locus_tag="VFR00_08920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018318008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEU0159424.1"
                     /translation="MAQRKMSRRQFVGTTAATASLLAAPFVRTAGAAGSLSLALWDHW
                     VPGGNTAMTEICQAWADKEKVNLQIDYLSTQGNKLYLTIAAEALAKSGHDIMDFSAWE
                     PPHYETSLEPVDDLMQEVLSRNGPVSPAIEYLAKPKGKWVVVPGIRGTLCLTACSRFD
                     LMKEHAGIDLLAMFPAGGEPSKAAEDWTWDNFLIAAERCHKAGYPFGLPLGGTTDSVD
                     WLGAFFRAFGAELVNEKGEITAKSDAMRQALEFLHRLSAFLPADVSAWDNASNNKWLV
                     AGKGALIFNPPSAWAVAKRDAPQVAEKLWTHAMPRGPKGRFVSVLPRFQGIWNFSKNK
                     AAAKALLHHVSTREAAAKLVDGGQGYDLPPFVKFNDFKTWDEQVPPKGTLSHYPNKGD
                     QVSIVPFAPAPPGVAQQIYTQALASKMVVRMMKGEPMPKTLDWAVAELEGFSRN"
BASE COUNT          373 a          692 c          725 g          343 t
ORIGIN      
        1 cggaaagcaa ggttgcctac tggagcgcgc agctggctcg gcaaaagacc ctgagcgagc
       61 gaggcatcgt ggcctcgagc aagttcgagg aagtcgagag caacgccacg gcggccgagg
      121 atcgcgtcgc cgtcatgcgc aagagggtcg atcgcgtcgc cgcgcaactg gggggcgatc
      181 ccgcccgcgc ggaggatgac cacccgatgg tgcggcagaa gcgggccgaa cgcgagcggg
      241 cggcgctcga tctggcgcac acggtgatcc gggctccgtt gggcgggacg gcggtcaacg
      301 tgcgcctgca gcgaggcgag caaatcaagg tggcgacgcc gctgtttgct ctggtggccg
      361 acacgcggcc gtgggtggag gccaatttca aggagaccga gctgacgcac gtgcgcaagg
      421 gccagaccgc actcgtcgtg ctcgatatct accccgacgt ggtctggcag gccgaaatcg
      481 aaagcataag ccccgcgacc ggcgccgaat tcgccgtgct gccgccgcaa aacgcctcgg
      541 gcaattgggt caaggtggtg cagcggctgc cggtgcgcct gcggctcttg ccgcaagcgg
      601 gcgagccgcc gctgcgcgcc ggcatgaccg cgagcgtgcg cgtggatacc gagcgccaga
      661 gccgactgag ccggctgctc gggggctggt cggcgttcgc gaccagccgc aggtagaggg
      721 gcctcagttc cggctgaacc cctccaactc ggcgacggcc caatccaagg tcttcggcat
      781 cggttcgccc ttcatcatgc gtaccaccat cttggaggcc agcgcctgcg tatagatctg
      841 ctgggccaca cccggcggcg ccggcgcgaa cggcacaatc gacacctgat cgcccttgtt
      901 gggatagtgc gaaagcgtgc ccttgggcgg cacctgctcg tcccaggtct tgaaatcgtt
      961 gaacttgacg aaaggcggca ggtcgtagcc ctgacccccg tcgaccagct tggcggcggc
     1021 ctcccgcgtc gagacgtgat gcaatagcgc tttggccgcc gccttgttct tggaaaaatt
     1081 ccaaatgccc tggaagcgcg gcagcacgga aacaaacctg cccttgggac cccgcggcat
     1141 ggcgtgcgtc cacagtttct ccgccacctg cggggcgtcg cgtttcgcca ccgcccaggc
     1201 gctgggcggg ttgaaaatca gcgcgccctt gcccgccacc agccatttgt tgttggaggc
     1261 attgtcccag gcgctgacat cggccggcag aaaggccgac aggcggtgga gaaactcgag
     1321 cgcctggcgc atggcgtcgg actttgccgt gatctcgccc ttctcgttga cgagttcggc
     1381 gccgaacgcg cgaaagaagg cgccgagcca gtcgacactg tccgtcgtcc caccgagcgg
     1441 cagcccgaac gggtagcccg ccttgtgaca cctctcggcc gcgatcagga agttatccca
     1501 ggtccagtcc tcggccgcct tgctgggctc gccgcccgcc ggaaacatgg ccaacagatc
     1561 gatgccggcg tgctccttca tcagatcgaa acggctgcag gcggtcagac acaaggtgcc
     1621 gcggatgccg ggcaccacca cccacttgcc cttgggcttg gcgaggtatt cgatcgccgg
     1681 actgacgggt ccgttgcggc tcaatacctc ctgcatgagg tcgtcaacgg gctcgagcga
     1741 ggtctcgtag tggggcggct cccaggcgga gaagtccatg atgtcgtgac cggacttggc
     1801 aagagcctcc gccgcaatcg tcaggtaaag cttgttgccc tgggtcgaga gatagtcgat
     1861 ctgcaaattg accttctcct tgtcggccca ggcctggcag atctccgtca tggcggtatt
     1921 gccgccgggg acccagtggt cccaaagggc aaggctgagg cttccggccg caccagccgt
     1981 gcgcacgaat ggcgcggcca agagcgaggc cgttgccgcc gtcgtgccga cgaattgacg
     2041 acgggacatc ttgcgctggg ccatcaggta cctccaggaa acgagattgg atcggggctg
     2101 agcgagccga tcggcggcct gcccggcgcc ggc
//
LOCUS       DASTMT010000381         2213 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_4145429, whole genome shotgun sequence.
ACCESSION   DASTMT010000381 DASTMT010000000
VERSION     DASTMT010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2213)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2213)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2213
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4145429"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..654)
                     /locus_tag="VFR00_08925"
     CDS             complement(<1..654)
                     /locus_tag="VFR00_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006021303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HEU0159425.1"
                     /translation="MYAFDYHRPSSVAEAARLLAGSQEGKLLAGGHTLLPTMKLRLAG
                     PKHIIDLGRISELRGIERKGGDLVIGAMETHDAVASSPVVQGAIRGLAELAAHIGDQH
                     VRHRGTIGGSVANNDPAADYPAAVLALNATLVTNKREIKADDFFKGLFTTALGADEII
                     TRITFPIASKFAYAKFANPASRYALVGVAVAQPPSGVRVAVTGASAAGVYRASDMEAA
                     "
     gene            complement(677..>2213)
                     /locus_tag="VFR00_08930"
     CDS             complement(677..>2213)
                     /locus_tag="VFR00_08930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018261278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEU0159426.1"
                     /translation="RAVKWTGDRSEAFLADAHGRDHATHAELALDADGKILALRANTK
                     ANLGAYLSTFASSVPTYLYAPLLSGQYNIPAIYCEVDGVYTNTAPVDAYRGAGRPEAT
                     FVVERLVEVAARQIGMDPAEFRRQNFIKSFPHQTPVIMAYDAGDYEACLNKALELADY
                     KGLAARKADSAKKGLKRGLGFSAYIEACGIAPSQAVGSLGAGVGLWESAEVRVNPTGS
                     VEVLTGSHSHGQGHETTFAQLVSQRLGIPIEQITVVHGDTDKVQFGMGTYGSRSGAVG
                     MSAIFRAIDKVIVKAKKVAGFVLEVPEDSVDFNDGIFSSKATNKTLTFPEVALQAYIA
                     HKFTGAQLEPGLKEGAFFDPTNFTFPSGVHICELEIDPATGATHIDRWTAVDDFGTLV
                     NPMIVEGQVHGGIVQGVGQALLEGAVYNKDGQLVTASFMDYTMPRASDVPSFKVAMTS
                     TPCPSNPLGIKGCGEAGAIAAPAAVINAITDALGHEGVAMPATPQAVWRAANSSMQKL
                     AAE"
BASE COUNT          360 a          676 c          768 g          409 t
ORIGIN      
        1 ggcggcttcc atgtcgctgg cgcgatagac gcccgcggcg gaggcccccg ttaccgccac
       61 tctcaccccg ctcggcggtt gcgcaacggc aacgcccacg agggcatagc gcgaggcggg
      121 attggcaaat tttgcgtagg caaacttgct ggcgatggga aaggtgatgc gggtgatgat
      181 ctcgtcggcg ccgagtgcgg tggtgaacag tcccttgaag aaatcatcgg ccttgatttc
      241 acgcttgttg gtgacaagcg tggcgttgag cgccagcacg gcggctggat aatcggcggc
      301 tggatcgttg ttggcgaccg atccgccaat ggtgccgcga tggcgcacgt gctggtcgcc
      361 gatgtgggcg gccagttcag ccaggccacg aatggccccc tgcaccacgg gcgagcttgc
      421 cacggcgtcg tgcgtctcca tggcgccgat gacgagatcg ccgcccttcc tttcgatgcc
      481 gcgcaattcg gagatgcggc cgagatcgat aatgtgcttc ggtccggcca ggcgcagctt
      541 catggtgggc agcagcgtgt ggcctcccgc cagcagcttg ccctcctgcg agccggccag
      601 cagcctggcg gcctcggcaa ccgagctcgg acggtggtaa tcgaaggcat acatgatggg
      661 ttcctccgtg ctcgcgctat tcggcggcaa gcttttgcat tgaactattg gcggcgcgcc
      721 agactgcctg tggcgtggcc ggcatggcta caccctcgtg ccccagcgcg tccgtgatgg
      781 cgttaatgac ggcggccggt gcggcgatgg cgcccgcctc gccgcaaccc ttgatgccca
      841 gcgggttcga tggacatggt gtgctggtca tcgccacctt gaaggacggc acgtcgctgg
      901 cgcgcggcat ggtgtagtcc ataaagctgg cggtaacgag ctggccgtcc ttgttgtaaa
      961 cggcgccttc gagcagcgcc tggcccacgc cctgcacgat gccgccgtgc acctggccct
     1021 cgacgatcat cggattgacg agggtgccga aatcatcgac cgccgtccag cgatcgatgt
     1081 gggtggcgcc cgtggcggga tcgatctcga gctcgcagat gtgcacgccc gacgggaacg
     1141 tgaagttggt gggatcgaaa aaggcacctt ccttgagccc gggttcgagc tgggcgccgg
     1201 tgaacttgtg ggcgatgtag gcctgcaagg ccacctcggg gaaggtgagg gtcttgttgg
     1261 tggccttgct cgagaagatg ccgtcgttga agtcgacgct gtcttcggga acttcgagca
     1321 cgaagccggc caccttcttg gccttgacga tgaccttgtc gatggcgcgg aagattgccg
     1381 acatgccgac cgcgcccgag cgcgagccgt aagtgcccat gccgaactga accttgtcgg
     1441 tatcgccgtg cacgaccgtg atctgttcga tgggaatgcc gaggcgctgc gagaccagct
     1501 gcgcgaacgt ggtctcgtgg ccctgaccat ggctgtgcga gccggtgagc acctcgacgg
     1561 agcccgtggg attgacgcgc acttccgccg attcccacag gccgacgccg gcgccgagcg
     1621 aacccacggc ttggctcgga gcaatgccgc aggcctcgat gtaggcggag aagccaagcc
     1681 cgcgtttcaa gccctttttc gcgctgtcgg ccttgcgcgc ggcgaggccc ttgtagtcgg
     1741 caagctcgag cgccttgttg aggcaggcct cgtaatcgcc ggcgtcgtag gccatgatca
     1801 ccggggtttg atgcgggaag gacttgataa agttttgccg gcgaaactcg gccggatcca
     1861 tgccgatctg ccgggccgcc acctcgacca gacgttccac cacgaaggtc gcttccgggc
     1921 gtccggcgcc gcgataggca tcgacggggg cggtattggt gtagacgccg tcgacctcgc
     1981 aatagatcgc cgggatattg tactggcccg acagcagcgg tgcgtagaga taggtcggca
     2041 ccgacgaggc gaaggtcgag agataggcgc cgaggttggc cttggtgttc gcccgcagcg
     2101 ccaggatctt gccgtcggcg tccagcgcga gttcggcatg cgtggcgtgg tcacggccgt
     2161 gcgcatcggc gaggaaggcc tcgctgcgat cgccggtcca tttcaccgcg cga
//
LOCUS       DASTMT010000382         6135 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_541168, whole genome shotgun sequence.
ACCESSION   DASTMT010000382 DASTMT010000000
VERSION     DASTMT010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 6135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6135
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_541168"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            93..914
                     /locus_tag="VFR00_08935"
     CDS             93..914
                     /locus_tag="VFR00_08935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015927540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEU0159427.1"
                     /translation="MQFETILVERAHGVARMTLNRPARANALSQAMLSEIGQALDALE
                     ADGDVRALVVRGAGATFSSGFDLKEQLERRPVGRAQWQPILRKDFDTVMRFWHFPKPT
                     IAAVRGACLAGACELALACDMTIAAPDAFFGEPELKFGAGIVVMILPWIVGPKVAKEI
                     ILTGEDRIDAERALAIGMINRIVPAEDLEAASLRLARHVAAVDPELVKATKRAINRAL
                     EAQGMLAALEDALAIDLGIEGEGSPDKARFMEVARRDGLKAALAWRDARFPKEGS"
     gene            911..2356
                     /locus_tag="VFR00_08940"
     CDS             911..2356
                     /locus_tag="VFR00_08940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015927539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid--CoA ligase family protein"
                     /protein_id="HEU0159428.1"
                     /translation="MTASLAEHLHAALSRSAQRTSPEGASGTIAGSGELLAAEAVLSA
                     AETLARSLRKAQIEPGEPVLCLIGNTPRDLAVFLGTWLAGGVAVPLAAAAAPTSLEQV
                     RRATAARLSVRGGELEDIARPVPPARDLLRSAALIVFTSGSTGEPKGVVLGGPRLLGK
                     LQVLDRLLAFGRDDSVVVPLQLGFIFGIWVSLLAILSGARLHLVAKFSAEALAARLAA
                     GASVVAVVPSMLRALLAPQRAPAAPRLRMLLSGGESLGHQLNTNARAAFPHTSIYDLY
                     GLTETGACDFCLPPNEIGAGVGSIGTPTEGVDYRIVDASGAPVKPGRTGELLIKTPFA
                     MLGYLDAPQLSAAAYLEGYLRSGDQARLRADGHVEIVGRLKDIISRGGNKIAPAEIDQ
                     LLLRHPAISGALCAGIPDARLGEIIGAMVTLAPGARVTGEELRSWAAGQIEKFKVPDV
                     IHICDSLPTGSTGKLSRAAVARRLAAPRARP"
     gene            complement(2372..4048)
                     /locus_tag="VFR00_08945"
     CDS             complement(2372..4048)
                     /locus_tag="VFR00_08945"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEU0159429.1"
                     /translation="MAVKSDIEIAREAKMQPIAEVGRKIGIPADALLNYGPYKAKVSF
                     DFINSAQKNKDGKLILVTAISPTPAGEGKTTTTVGLGDGLNRIGKKAMSCLREPSLGP
                     CFGMKGGAAGGGYAQVVPMEDINLHFTGDFHAITSAHNLLSALIDNHIYWGNPAGLDS
                     RRVAWRRVMDMNDRALRSIVNSLGGVANGFPREDGFDITVASEVMAILCLAMDLNDLE
                     RRLGNIVVGYTRERKPVKAADVKGDGAMTVLLKDALMPNLVQTLENNPVFIHGGPFAN
                     IAHGCNSVLATRTALKLCDYVVTEAGFGADLGAEKFFDIKCRKAGLNPAAAVIVATVR
                     ALKMHGGVGKDDLKHENAAAVAKGCDNLKRHLENVRKFGVPPIVAINRFVTDTDAEMA
                     EVMRAAESAGTKAYVCTHWGDGGKGIEALARQVVEVADSGQAKFKPLYADAVPLRDKV
                     KTIATEIYHAADIACDGAIEARFKELQEAGYGHLPVCMAKTQYSFSTDPNKRGAPSGH
                     IVPIRELRLSAGAEFVVVITGEIMTMPGLPRVPAANSIRLNQQGQIEGLF"
     gene            complement(4088..5029)
                     /locus_tag="VFR00_08950"
     CDS             complement(4088..5029)
                     /locus_tag="VFR00_08950"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009465468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEU0159430.1"
                     /translation="MVMRIVVHGQEAFGKAVLEKLLERKENVVAVFCAPDKAGRPEDP
                     LKVFAKEKGLPVHQPKSWKTPEALELMKSANADLCVMAYVTLFVPQPVLDAPKLGTIQ
                     YHPSLLPLHRGPSSINWPIAMGRTETGLSIFWPDEGLDEGPILLQKKVAIGPDETLGD
                     VYFKKLFPLGVDAMMESIDLVRAGKAPRQAQDHAKKTYESWFKKDVAEIDWAKPVAEV
                     YNLIRAANPAPGAWGVLNGQKLDLFDSGKVSGSGKPGEILQLDANGMTVAAGGGAILV
                     KRVRGADGKKVAAGEWAASAGVKAGDSFAKPAAKSIS"
     gene            complement(5078..>6135)
                     /gene="frc"
                     /locus_tag="VFR00_08955"
     CDS             complement(5078..>6135)
                     /gene="frc"
                     /locus_tag="VFR00_08955"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012043249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033608 - formyl-CoA transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009636 - response to toxic substance
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HEU0159431.1"
                     /translation="SKNKKGMEILERLVKTCDVLVENFAPGALDRMGLTWERIQELNP
                     RMVYASVKGFGPGPYEDCKVYENVAQCTGGSASTTGFRDGPPLVTGAQIGDSGTGLHL
                     ALGIVTALYHRNTSGRGQRVTCAMQDGVLNLARVKMRDQQRLAHGPLSEYSQYGEGIP
                     FGDAVPRAGNDSGGGQPGRILKCKGWETDPNAYIYFITQAPVWEAICDVIGEPGWKTH
                     PDYAKPPARLPRLNEIFGRIEQWTMTKSKFEAMEILNKYDIPCGPILSMKELAEDDSL
                     RATGTIVEVDHPARGKYLSVGNPIKLSASPSEVKRSPLLGEHTGEVLAELGYSSDEIA
                     ALAQAGATAAQKKQAAE"
BASE COUNT          977 a         2041 c         1989 g         1128 t
ORIGIN      
        1 cttttcaatc gttgaaggcg ctggagcgga tgcgcgcgga caccggtcgc tagcgcgcca
       61 ggccctggcg tgaacacagc ggagggcgtg aggtgcaatt cgagaccatt ctggtcgagc
      121 gggcccacgg cgtggcgcgc atgacgctca atcggccggc gcgcgcgaat gctttgagcc
      181 aggcgatgct cagcgagatt gggcaagccc tcgacgccct cgaggccgat ggcgacgtgc
      241 gcgccctcgt ggtgcgcggg gccggcgcaa ccttttcctc cggcttcgat ctcaaggagc
      301 agctagagcg gcgcccggtg ggcagagctc agtggcagcc gatcctgcgc aaggatttcg
      361 acacggtcat gcgcttttgg cattttccca agcccacgat cgccgccgtg cgcggcgcct
      421 gcctcgctgg cgcctgcgaa ctggcgttgg cctgcgacat gaccatcgcc gcgccggatg
      481 cattcttcgg cgaaccggag ctgaagttcg gcgccggcat cgtcgtcatg atcctgccct
      541 ggatcgtggg accgaaagtg gccaaggaga tcatcctgac cggcgaggat cggatcgatg
      601 ccgagcgtgc gcttgccatc ggcatgatca accgcatcgt gccggccgag gacctcgagg
      661 ccgcgagcct tcggctggcc cgccacgttg ccgcggtcga tccggaactg gtcaaagcaa
      721 ccaagcgcgc catcaatcgt gcgctcgaag cgcagggcat gctcgcggcg ctggaggacg
      781 cacttgccat cgatctgggc atcgaggggg agggctcccc cgacaaggca cgcttcatgg
      841 aggttgcccg gcgcgatgga ctgaaggccg cgctggcttg gcgggacgcc cgctttccca
      901 aagagggatc atgacggcaa gccttgcgga gcatttgcac gccgcgctga gccgcagcgc
      961 ccaacgaaca tccccggagg gagcgtcggg cacgatcgcg ggcagcggcg agctattggc
     1021 cgccgaggcg gtgctgtcgg cggccgagac gctggcgcgc agcctacgca aggcccagat
     1081 cgagcccggc gagccggtgc tctgcctgat cggcaacacg ccgcgcgacc tcgccgtttt
     1141 cttgggcacc tggctcgccg gcggggtggc cgtacctctc gccgcggcgg cggccccgac
     1201 aagcctcgag caggttcgac gcgcaactgc agcccgtctg tcggtccgtg gcggggagct
     1261 cgaagacatc gcgcgcccag tgccaccggc gcgggacctt ttgcgcagcg cggcgctgat
     1321 cgttttcacg tcggggagca cgggcgaacc gaagggcgtg gtgctcggcg gcccgcgctt
     1381 gctgggcaaa ctgcaggtcc tcgatcgcct gctcgcgttc gggcgcgacg acagcgtcgt
     1441 cgtccccctg caactgggat ttattttcgg gatctgggtc agcctgctcg ccatcctgag
     1501 cggggcccga ctgcacctgg tggcaaagtt cagcgccgag gcgctcgcgg caaggctcgc
     1561 tgccggagcc agcgttgtgg ccgtcgtgcc ctcgatgctg cgagcgctat tggcgccgca
     1621 gcgcgccccc gccgcgcccc gtctgcggat gctcctcagc gggggcgaaa gcctcggcca
     1681 tcagctcaac accaatgcac gtgccgcctt tccgcacacg tcgatctatg atctttacgg
     1741 tttaaccgag accggagcct gcgacttctg cctgccgccg aacgaaatcg gggccggcgt
     1801 tggctcgatt gggacgccga ccgaaggggt ggactaccgc atcgtcgatg ccagcggcgc
     1861 gccggtaaag ccagggcgga cgggggaact attgatcaag acgcccttcg ccatgctggg
     1921 ttacctcgat gctccgcagc tcagcgccgc cgcctatctc gagggatatt tgcgcagcgg
     1981 cgaccaggcg cgcctgcgcg ccgacgggca cgtcgagatc gtgggccgtt tgaaggacat
     2041 catctcgcgc ggcggcaaca agattgcccc cgccgagatc gatcagctct tgctgcgcca
     2101 tcccgcgatc agcggcgcgc tttgtgcggg catacccgac gcgcgtctcg gcgagatcat
     2161 cggcgccatg gtgaccctgg cccccggcgc ccgcgtgacc ggggaggaat tgcgcagctg
     2221 ggccgccgga cagatcgaga agttcaaggt tcccgacgtc atccatatct gcgacagcct
     2281 gccgaccggc agcacgggca aattgagccg cgccgcggtg gcgcggcgcc tggcggcacc
     2341 tcgtgccagg ccttaggcca acgcgcaggg ctcagaagag accttcgatt tgcccctgct
     2401 ggttgaggcg aatgctattg gcggccggca cccgcggcag gccgggcatg gtcatgatct
     2461 cgcccgtgat caccaccacg aactccgcgc ccgccgacag ccgtagctcc ctgatcggca
     2521 cgatgtgtcc gctaggtgcc ccgcgcttgt tgggatccgt cgagaacgaa tactgcgtct
     2581 tggccatgca caccggcagg tgcccgtaac cggcctcctg cagctccttg aagcgggcct
     2641 cgattgcacc gtcacaagcg atatcggcgg cgtgatagat ctcagtggcg atggtcttga
     2701 ccttgtcgcg caggggcacc gcgtccgcgt agagcggctt gaatttcgct tgcccgctgt
     2761 cggcgacctc caccacctgc cgtgccagtg cctcgatgcc cttaccgcca tcgccccagt
     2821 gcgtgcacac ataagccttg gtgccggcgc tttcggcggc ccgcatcact tccgccatct
     2881 ccgcgtcggt gtcggtgaca aaacggttga tggccacgat cggcggcacg ccgaacttgc
     2941 gcacgttttc caggtgccgc ttgagattgt cgcagccctt ggcgacggcc gcggcgttct
     3001 cgtgcttcag gtcatccttg ccgacgccgc cgtgcatctt gagcgcgcgc acggtggcaa
     3061 cgatgaccgc tgccgccgga ttgagccccg ccttacggca cttgatgtcg aagaactttt
     3121 ccgcgccgag gtcggcgccg aagccggcct cggtaaccac gtaatcacac agtttgaggg
     3181 ccgtgcgcgt tgccagcacg gagttgcagc cgtgcgcgat attggcgaac ggaccgccgt
     3241 ggatgaagac cgggttgttc tccagggttt gcaccaggtt cggcatcagc gcgtccttca
     3301 gcagcacggt catggcgcca tcgcccttga cgtcggccgc tttgaccggc ttgcgctcgc
     3361 gggtatagcc aacgacgatg ttgccgagcc gccgctcgag gtcgttcaga tccatcgcca
     3421 agcagaggat ggccatcacc tcggaggcca ccgtgatgtc aaaaccatcc tcgcggggaa
     3481 agccgttggc cacgccgccc agcgaattca cgatcgagcg cagcgcgcgg tcgttcatgt
     3541 ccatgacgcg gcgccaggcc acgcgccggc tgtcgagccc ggcgggattg ccccagtaga
     3601 tgtggttgtc gatcagggcc gagagcaggt tgtgggccga agtgatggcg tggaaatcgc
     3661 cggtgaagtg caagttaatg tcctccatcg gcaccacctg cgcgtagccg ccgccggccg
     3721 caccgccctt catgccgaag caggggccga gcgaaggctc gcgcaggcag ctcatggctt
     3781 tcttgccgat gcgattgaga ccgtcgccga gccccaccgt ggtggtggtc ttgccctcac
     3841 cggcaggcgt cgggctgatc gccgtgacga ggatcagttt gccgtccttg ttcttctgcg
     3901 ccgaattgat gaagtcgaac gagaccttgg ccttgtaggg gccatagttg aggagcgcgt
     3961 cggctggaat accgatcttt ctgccgacct ccgcgatcgg ctgcatcttc gcctcgcgcg
     4021 cgatttcgat atccgacttc acggccatga ctttccctcc acagaatttc gcgcgtttgt
     4081 gagaagacta actgatcgat ttcgccgccg gtttggcaaa actgtcgccc gctttgacgc
     4141 cggcggaggc agcccattcg ccggcggcga ccttcttgcc gtcggcaccg cgcacgcgtt
     4201 tcaccagaat ggcgccgccg ccggcagcaa ccgtcatgcc attggcatca agctggagga
     4261 tctcgccggg cttgccgctg ccgctgactt taccggagtc gaagaggtcg agcttttgcc
     4321 cgttgagcac gccccaggcg cccggcgccg ggttggccgc gcggatcagg ttgtagacct
     4381 cggcgacggg ctttgcccaa tcgatctccg ccacatcctt tttgaaccaa ctttcgtagg
     4441 tcttcttggc gtggtcctgc gcctgcctgg gtgccttgcc cgcgcgcacg agatcgatgc
     4501 tttccatcat ggcatcgacg cccagcggga acagcttttt gaaatagaca tcgcccaagg
     4561 tctcatccgg gccaatcgct accttcttct gcagcaggat cgggccttca tccaggccct
     4621 cgtcgggcca gaagatggaa agccccgtct ccgtcctgcc catggcgatg ggccaattga
     4681 tcgaggaggg gccgcggtgc aggggcaata gcgagggatg atactggatg gttccgagct
     4741 tgggtgcgtc cagcaccggc tgcggcacga acagggtcac gtaggccatc acgcacaaat
     4801 cggcgttggc cgacttcatg agttcgagcg cctccggggt cttccagctc ttgggctggt
     4861 gcacgggcag ccccttctcc ttggcaaaca ccttgagtgg atcctcgggg cggccggcct
     4921 tgtcaggcgc gcagaacacc gccaccacat tttctttgcg ctcgagcagc ttctccagca
     4981 cggccttgcc gaaggcctcc tgcccatgca ccacgatccg catcaccatg cctccgctcg
     5041 gttcacgttg ccgccccgcc gcttggggct ccaattgcta ctcggccgcc tgttttttct
     5101 gcgccgccgt ggcgcccgct tgcgccagcg ccgcaatctc gtcggaggag tagccgagtt
     5161 cggccaagac ctccccggtg tgctcgccga gcagcgggga tcgcttcacc tccgaaggtg
     5221 atgcggagag cttaatcgga ttgccgaccg acaagtactt gccgcgcgcc ggatggtcca
     5281 cctcgacgat ggtaccggtc gcacgcagcg aatcgtcctc cgccaattcc ttcatggaga
     5341 ggatcggccc gcagggaatg tcgtacttgt tcaggatctc catggcctcg aatttgcttt
     5401 tcgtcatggt ccattgctcg atccggccaa aaatctcgtt gaggcggggc aagcgcgccg
     5461 gcggcttggc gtagtcggga tgcgtcttcc agccgggctc gccgatcacg tcgcagatcg
     5521 cctcccagac gggagcctgc gtgatgaaat agatgtaggc attgggatcg gtctcccagc
     5581 ccttgcattt caggatgcgt ccgggctggc cgccgccgga atcgttgccg gcgcgcggca
     5641 ccgcatcgcc gaacgggatg ccttccccat actggctgta ctcgctgagc ggcccatgcg
     5701 cgaggcgctg ctgatcgcgc atttttacgc gcgcgaggtt gagcacgccg tcctgcatgg
     5761 cacaggtgac gcgctgtcca cggccgctcg tgttgcgatg atagagcgcc gtgacgatgc
     5821 cgagcgccag atgcaagccg gtaccgctgt cgccgatctg ggcgccggtg acgagcggcg
     5881 gcccgtcccg gaagccggtg gtggaggccg agccaccggt gcactgggcc acgttctcat
     5941 aaaccttgca gtcctcgtac ggcccgggcc cgaaaccttt caccgaggca tagaccatgc
     6001 gcggattgag ctcctggata cgctcccagg tgagacccat gcggtcgagc gcgccgggcg
     6061 cgaaattctc caccagcacg tcgcacgtct tcaccagccg ctcgaggatc tccatgcctt
     6121 tcttgttctt gctgt
//
LOCUS       DASTMT010000383        14049 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_1521408, whole genome shotgun sequence.
ACCESSION   DASTMT010000383 DASTMT010000000
VERSION     DASTMT010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 14049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14049
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1521408"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..539
                     /locus_tag="VFR00_08960"
     CDS             <1..539
                     /locus_tag="VFR00_08960"
                     /inference="COORDINATES: protein motif:HMM:NF014042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043743 - LPPG:FO 2-phospho-L-lactate
                     transferase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2-phospho-L-lactate transferase CofD family
                     protein"
                     /protein_id="HEU0159432.1"
                     /translation="FQEYFVREKCRPCLRQVRFEGAAEAKPSRKVLAALADPALAGIV
                     LCPSNPWLSIAPILAVAGLKGALLAAGVPIVAVSPIIAGQAVKGPAAKIMAELGLAPD
                     SRSIAGYYAGFVDGLLIDEADAALAADIPLAVKVTKTLMQTLDDRIALARTCLSFCAE
                     LARARASPSGKGCGASVP"
     gene            536..1228
                     /gene="cofC"
                     /locus_tag="VFR00_08965"
     CDS             536..1228
                     /gene="cofC"
                     /locus_tag="VFR00_08965"
                     /EC_number="2.7.7.68"
                     /inference="COORDINATES: protein motif:HMM:NF014085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043814 - phospholactate
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate guanylyltransferase"
                     /protein_id="HEU0159433.1"
                     /translation="MMGAGTWAILPVKVLGLAKQRLAPVLPPAARRELVLLMLRRVLA
                     AVAAVDRLGPILVVTPDTQVADLAKAEGALVLAEARASGLNAAVRRGLAHALAKGAAR
                     ALVLPADVPLVTPDELAALLGNMAVGGGARASLVPAGDGEGTNAMLLDPPDALAPSFG
                     PGSYVRHLAQALARRLDVEVLHLPGLAADIDRPADLARLLGPAPELQPFQFLRAHVMK
                     AGGDGRTGRECP"
     gene            1225..3684
                     /gene="cofH"
                     /locus_tag="VFR00_08970"
     CDS             1225..3684
                     /gene="cofH"
                     /locus_tag="VFR00_08970"
                     /EC_number="2.5.1.147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013168998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(D-ribitylamino)uracil--L-tyrosine
                     4-hydroxyphenyl transferase CofH"
                     /protein_id="HEU0159434.1"
                     /translation="MTTAMMAEALAAADAGRRLSPTHALALSDCEDTGRLSALAEALA
                     IAGHGRRLSFSKKVFIPLTQLCRDVCHYCTFAQTPRKTEPYLTSEAVLAIAEAGQAAG
                     CKEALFTLGDKPELRYAAARRALSQLGAASTLDYLEATAARVLSATGLLPHLNPGLLD
                     ETWLRRLKKVSLSQGLMLETAALRLSAPGGPHFGSPDKHPARRLATLEAAGRARVPFT
                     TGLLIGIGETRRERIEALLAIRELHERYGHIQEIIIQNFRAKPGTRMASAPEPSLSEH
                     LWTIAVARIIFGAAMNIQAPPNLQPAALAEVIGAGINDWGGVSPVTPDHVNPEAPWPH
                     LQGLREATERAGRTLIERLAAYPEVLLAEAAPTGGEGNGSPARAGGARYLTTPWLEDA
                     LRTPTLRLMDGQGYAREDGWVAGALTAVSAAPWAAPGVGPAKESPLKRQLARALAGEA
                     LAEDDIVGLFAARGREFAAVVAAADTLRARSVGDTVTYVVNRNINYTNICTYGCKFCA
                     FSKGRHNLGHRDAPYDLDAGEIGRRTLEAWQRGATEVCLQGGIRPGYTGETYLGIVAA
                     VKSAVPQMHVHAFSPLEIWHGATTLGLSLRAYLGRLEAEGLASLPGTAAEILDDEVRA
                     ILCPDKINTAQWFEVMETAHSVGLKSTATIMFGHVDGYRHWARHLIGVRRLQAKTKGF
                     TEFVPLPFVHMEAPIYLKGQARKGPTLREALLMHAVARLALYPEIVNIQASWVKMGPE
                     GAVACLAAGANDLGGVLMNESITRAAGAMHGQEIGASDLAAMIRAVGRRPEQRNTLYA
                     VLRGDSGGGPGRQSPPLEAAE"
     gene            3705..5201
                     /locus_tag="VFR00_08975"
     CDS             3705..5201
                     /locus_tag="VFR00_08975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018389531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEU0159435.1"
                     /translation="MTPFDFPRRVEDASVRARAVGLLKARGVRLPTFAELADPHPLPA
                     AARGALASVDPDQPDAANLARVNWFNDWDRRTRRATPAYLEIPRALSGVEAPILVAIG
                     DLFPMISAHKVLAAYACLAPRLAAGRFDPTRQRAVWPSTGNYCRGGVAISRILGCRGV
                     AVLPAGMSQERFDWLGQWVSAPEDIIRTPGTESNVKEIYDACAELARDPSLEIINQFS
                     EFANYLVHWRCTGPALEKIFETHAQASTLTLAGFVAASGSAGTLAAGDYLKHRHGAKI
                     AVVEALECPTLASNGYGEHNIQGIGDKHVPLIHNVMNTDLVIGVSDRATDALNAVFNS
                     AAGRAHLLQRFDLSPQFAEHLSHFGLSSIANILGAIKMAKHLALGPDSAILTVATDGH
                     GLYGSELKRFLARRHNSDVLSERFAAELTGQYLMGTGSENVLEATTRERQRMFNLGYF
                     TWVEQQGIAVADFDRRRQSQFWQDLQGLLPLWDEMIAAVNCDSGQVAA"
     gene            5221..6192
                     /locus_tag="VFR00_08980"
     CDS             5221..6192
                     /locus_tag="VFR00_08980"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015241776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HEU0159436.1"
                     /translation="MSSTAQVAPYSELRRATLARDRGLREKVMSLEAAAQLVRDGEHV
                     AIGGCTLSRTPMAMIWALIRAQKKNLVISRSITSTEGDLLLASGVSKHVITSWFSQGI
                     VWGVSKIMRHYTERKLARFEEWSHMSMGLRYRAGAMGIPFIPSRSMLGSDVGERVVEQ
                     MRSLTCPFTGEQLTLLPALNPDWALIHVQRCDAFGNAQLDGLQFMDIDIAMAAKRVIL
                     TTERIISNEQIRRVPDLTRIPFFAVEAVVEVPIGCAPHECYGVYEPFFGALDDYARRV
                     ARAPVSGCRPYLEEYYFSAKSWPDYLSKLGLDAMLDACRRGRSVHND"
     gene            6185..6967
                     /locus_tag="VFR00_08985"
     CDS             6185..6967
                     /locus_tag="VFR00_08985"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018184327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HEU0159437.1"
                     /translation="MTEEAQPPPHYTASELLAVMASRLLRDGQIVFAGVGVPLLAATL
                     AQRIHAPHLTILFEGGTIGPFIVPGQLPPSTNEQRCTRRANMVLPITDVLLLLQRGYV
                     DVGFMGGAQIDKFGNLNSSFIGDADDPEIRLPGSGGGNDISSLTDMIVAMKHEKRRFV
                     EKVDFITSPGWLAGQRTRAERGLPQGGMWRVVTDLATMGFDTDSREMKVLGLNPGVSR
                     EQVVDNTGFKLMFAENLEPVEPPRQRELAVLRELDPERLYTA"
     gene            6972..7775
                     /locus_tag="VFR00_08990"
     CDS             6972..7775
                     /locus_tag="VFR00_08990"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HEU0159438.1"
                     /translation="MAVQSSETLRNNVKEKLARNELVSSITVRLVRSIEIVRIAKTAG
                     FDSLYVDLEHSSLGLDATGQICLAALAIGIAPFVRVPANSPEYISRVLDGGALGVIAP
                     GVRSAAEARHVVAAAKYAPLGRRGASGALPHLNYANLPADEANAAMNAATMVMVQFES
                     AEAIDRAEEIAAVDGVDLIMIGTNDFLADIGLPGQFESPRVTDAYARTIAACRAHGKH
                     VGVGGLTSRPQRIAEFVKMGARYVSTGTDLAFLMAEASARARQVRALSL"
     gene            7903..8982
                     /locus_tag="VFR00_08995"
     CDS             7903..8982
                     /locus_tag="VFR00_08995"
                     /inference="COORDINATES: protein motif:HMM:NF014653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HEU0159439.1"
                     /translation="MKSSQEFVRVAAAEAEAFGQRLLQHHGVPPEEAAIVAHCLVRAD
                     LRGIDTHGLQFLPQYLARVRRGLINPRPVLEPRRVTPVAASLDGDNGFGFVVGMRAMR
                     EAMEMARGFGIGIVSAHRSTHFGMAAAYVLPALEAGFIAMVFSNASRAMPPWGGREAL
                     LGTNPLAAGAPAGSQVPFLLDMSPAVAARGKIRRAARRGEAIPPGYALDADGRPTTDP
                     AAALDGGVVLPIGGYKGSGLSMLMDIMGGVISGAACAGDVASQFLDYDRPQNVGHFFL
                     ALKPDLFVEIEDYRARMDRLVERVHQSPTAHGFTEVVMPGELEARLEAERRSQGVPYH
                     RKEFAALAAEAASAGLPPLPVLQAE"
     gene            8988..9746
                     /locus_tag="VFR00_09000"
     CDS             8988..9746
                     /locus_tag="VFR00_09000"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU0159440.1"
                     /translation="MRVGGYDIDVIVQGYPGKAVCHGGLGWSSVVLLRGQGRIALIDA
                     GSFGLRPLLLPRLKARALAPGDVTDLLLTHSHHDHAVNWTLFRHARIRIGAHELAWSV
                     KEPWGETPVPELYMRELETWPTLATIQDGEEVLPGIRAHMAPGHTPGHLIFVLEGREH
                     DVIFTGDAAKNRAELISRTADMTYDPRVTEKTIEMIWSFWRRRPGSIVVPGHDLPLAQ
                     ENGRPRYIGKREAAVTAWFGDDMERTTLFNLTVA"
     gene            9865..10701
                     /locus_tag="VFR00_09005"
     CDS             9865..10701
                     /locus_tag="VFR00_09005"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEU0159441.1"
                     /translation="MRLRNVVVAALLVPLLPGIGTAQQPLKTAVDGTFAPHAMPKLEG
                     GIQGFNVDLANEVGKRLKRPVEIQAMQFSGILPALQAGTLDFVAAPVTVTKERADNYI
                     FTEGYLNTDYQFLTKKGEPPITDLKQFKGKVIAVNKGAVYDSWARGLEAEIGWSVESF
                     GTQTDAVQAVLAGRAYANVAGNTAIQWAVKQQPQLQLSYLHSTGLVWAMPLRKDSLAL
                     RTELENAIECMKKDGTMATMHERWFGTKPEANSPAVTIYPGTGVPGTQGWDQTPREPK
                     CD"
     gene            10754..11485
                     /locus_tag="VFR00_09010"
     CDS             10754..11485
                     /locus_tag="VFR00_09010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014532014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEU0159442.1"
                     /translation="MAILTVSGLRKSFGANEVLRGIELSVAERELVFIIGPSGSGKST
                     LLRCLNRLEEPTAGSITFDGQEVTARATNLNRLRRSMGMVFQSFNLYPHMTALGNVTL
                     ALRRVLKKSRAEADALGLAALTGVGLADKASSYPNELSGGQQQRVAIARALALDPRIV
                     LFDEPTSALDPELVGSVLSVMRDLKARGMTMLVVSHELRFAREAADRVVFMDAGRIVE
                     EGAPETIFSAPREMRTKAFVAQLIR"
     gene            11490..12173
                     /locus_tag="VFR00_09015"
     CDS             11490..12173
                     /locus_tag="VFR00_09015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019404523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEU0159443.1"
                     /translation="MTAWQRFLETFFKPDMLAQYWPSIAHGVLVTLQIASAVVLTGLS
                     FGLLLAGLRAYRLLPLNIAIVVFVDVFRSLPPLVAILIIYFGLPGVGINLPSFVVLWL
                     VLSLVLTAFAEEIFWAGIISTPKGQWEAARSTGLGFSETLLFVVLPQAVRLGVPPLTN
                     RTIAITKNTALGTVIGVGEILNQATAAQSFSGNATPLMMGAIAYILLFLPVVAVGRWI
                     ETRFQWRRA"
     gene            12173..12841
                     /locus_tag="VFR00_09020"
     CDS             12173..12841
                     /locus_tag="VFR00_09020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019404524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEU0159444.1"
                     /translation="MQEIGQQFLNVEIMLKIWPLLMRGFATTLYLCALTIPLGLIGGL
                     GVALILLSRSRLTRWLVIALVDLFRAAPPLVLLILIYSGLPFAGIRLSPTAAVCLAFF
                     CNTSSYYGEIYRAGIESIGRGQWEAARSTGLNGFETLTFVVLPQAVRNVLPDLVSNTV
                     EVVKLTSIASVVSFPELLYSADMARSVTFNSSPVVLAAIVYLLLLWPLVRLISRLERG
                     VATH"
     gene            12862..13797
                     /locus_tag="VFR00_09025"
     CDS             12862..13797
                     /locus_tag="VFR00_09025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007593122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-carbamoyl-D-amino-acid hydrolase"
                     /protein_id="HEU0159445.1"
                     /translation="MSRIVTVAAAQLGAIQKAEPRQAVIARMLALMDEAKAKGADLVV
                     YPELALTTFFPRWYYEDRSEADLWFEREMPNAAARPLFERAQKHGLGMSFGYAELTAD
                     GHHFNTAILVDKSGAIVGKYRKVHLPGHAEFDARRTHQHLEKRYFEPGDLGFPVWRCL
                     GGIFGMCICNDRRWPETYRVMGLQGVEMIVLGFNTPSVNSQKGDEGPQKRIFHHKLSV
                     QAGAYQNSTWVVAVAKAGVEDGHPLFGCSLIVNPDGEIVAESKTEADEVIVHACDLDA
                     TRFGKETIFDFDRHRRIEHYGRITAQAGAELPKER"
     gene            13807..>14049
                     /locus_tag="VFR00_09030"
     CDS             13807..>14049
                     /locus_tag="VFR00_09030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013845589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HEU0159446.1"
                     /translation="MSYNFAELQPSQRYKLLCGLVVPRPIALVTSISREGIVNAAPFS
                     FFNVFSENPALLVLGLQHNEDGSPKDTTRNIHRSGEF"
BASE COUNT         2276 a         4565 c         4636 g         2572 t
ORIGIN      
        1 gcttccagga atatttcgtg cgcgaaaaat gtcggccttg cctgcggcag gtccgctttg
       61 agggtgccgc cgaggccaag ccgagccgaa aggtgctggc cgcgctggct gaccccgccc
      121 tcgccggcat tgttttatgc ccctcaaacc cgtggctcag catcgccccg atcctggcgg
      181 ttgcgggcct caaaggcgcg cttctggccg ccggtgtgcc catcgtcgcc gtctcaccca
      241 tcatcgccgg tcaggccgtc aagggcccgg cggccaaaat catggccgaa ctcggtctgg
      301 cgccggacag ccgcagcatt gccggctatt atgccggttt cgtcgatggc ttgctgatcg
      361 atgaggccga cgccgccctg gcggccgata taccgctcgc cgtcaaagtg acgaaaacct
      421 tgatgcagac gctcgacgac agaatagcgc tggcgcgcac gtgcttgagt ttttgtgccg
      481 agctggcgcg ggcgcgggca tccccctcag gcaaggggtg cggagcctcg gtgccatgat
      541 gggagcaggc acttgggcga tcctgccggt gaaagtgctc gggctcgcca agcagcggct
      601 ggcgccggtg ctgccaccgg ccgcgcgccg cgagctggtg cttttgatgt tgcgccgtgt
      661 gctggccgcc gtggcggcgg tcgatcgcct tgggccgatc ctggtggtga cgccggacac
      721 ccaggtggcc gatttggcca aggctgaggg ggccctcgtc ttggccgaag cgcgcgcgag
      781 tggactcaac gcggccgtgc gccgcggcct ggcgcacgcc cttgccaagg gcgccgcacg
      841 cgccctggtg ctgccggccg atgtgccgct ggtgaccccc gacgaacttg ccgccctgct
      901 tggcaatatg gcggtgggcg gcggcgcgcg ggcgagcctg gtgccggccg gcgacgggga
      961 ggggaccaac gccatgctgc tcgatccgcc cgacgcgctt gccccatctt tcggtcccgg
     1021 cagctacgtg cgccacctgg cgcaggcgct tgcccgccgg ctcgatgtcg aggttttgca
     1081 tctgccgggt cttgctgcgg acatcgatcg gcccgccgat ctcgcccggc tgctcggccc
     1141 ggcccctgag cttcaaccct tccagttcct ccgcgcccat gtcatgaaag ccggcggaga
     1201 cggacgaacg ggacgagagt gcccatgacg accgcgatga tggctgaggc actggctgcg
     1261 gcggacgccg ggcggcggct gtccccgacg cacgccttgg ccttgagcga ctgcgaggac
     1321 acgggccggt tgagcgccct tgccgaggcg cttgccatcg ccgggcacgg ccgacgcctt
     1381 tctttttcga aaaaggtctt cattccgctg acccagttgt gtcgcgacgt ttgccactac
     1441 tgcaccttcg cgcagacgcc gcgcaagacc gagccctatc tgacgtccga ggcggtgctg
     1501 gcgatcgcgg aagccgggca ggcggcgggg tgcaaggagg ccctctttac gctgggggac
     1561 aagcccgagc tgcgctacgc ggcggcgcgc agggcgctga gccagctggg ggctgcctcg
     1621 acccttgact acctcgaggc caccgcagcc cgcgtgctga gcgccaccgg cctcttgccc
     1681 catctcaatc ccggcctgtt ggacgagact tggctgcggc ggctcaagaa agtctccctg
     1741 tcgcagggcc tcatgctcga aacggccgcg ctgcggctgt cggcgccggg cgggccgcac
     1801 ttcggctctc cggacaaaca cccagcccgg cggcttgcca ccctggaggc ggccggacgg
     1861 gcgcgggtgc cgtttaccac cggccttctc attggcatcg gtgagacgcg tcgggagcgc
     1921 atcgaggcgc tgctcgcgat tcgcgagctg cacgagcgct acggtcacat tcaggagatc
     1981 atcatccaga atttccgggc caagcccggc acgcgcatgg ccagcgcgcc cgaaccttcg
     2041 ctcagcgagc acctgtggac gatcgccgtg gcgcgcataa tttttggcgc ggccatgaat
     2101 atccaggccc ctcccaacct gcagcccgcg gcgctggccg aggtgattgg cgccggcatc
     2161 aacgattggg gcggggtgtc gccggtcacg cccgatcacg tcaatccgga ggcgccctgg
     2221 ccgcatttgc agggcttgcg tgaggccacg gagcgggccg ggcgcacctt gatcgagcgg
     2281 ctcgctgcct atcccgaggt gttgctcgcc gaagcggcgc cgacgggggg cgagggaaac
     2341 ggcagccctg cccgcgccgg aggcgcgcgc tacctgacga cgccgtggct cgaggacgcg
     2401 ctcaggaccc cgacgctgcg cctgatggat gggcaaggct atgcccgcga ggatggctgg
     2461 gttgccggcg cgctgaccgc ggtgtcggcc gcgccgtggg cggccccggg cgtggggcca
     2521 gcgaaagaga gcccgctcaa gcgccaactc gcgcgtgccc tcgccggcga ggcgctcgcg
     2581 gaggacgaca ttgtcggcct ctttgctgcg cgcggccgcg agtttgcggc cgtcgtcgcc
     2641 gccgccgaca ccctgcgcgc ccggtcggtg ggggacaccg tcacctatgt cgtcaaccgc
     2701 aacatcaatt acaccaacat ttgtacttac ggctgcaagt tctgcgcctt ttccaaaggt
     2761 cgccacaacc tcggccaccg cgatgcgccc tatgatctcg atgccggcga gatcggccgg
     2821 cgcacgctgg aagcctggca gcgcggcgcc accgaggtgt gtctgcaggg cggtatccgg
     2881 cccggttata cgggagagac ttatcttggc atcgtcgcgg ccgtaaaatc ggcggtgccg
     2941 cagatgcacg tgcacgcctt ttccccgctc gagatctggc acggggcgac gacgctcggc
     3001 ctgtcgctgc gcgcctacct cgggcgcctg gaggctgagg gcctcgcgag cctacccggc
     3061 acggccgccg aaatcttgga cgatgaggtg cgcgccatcc tctgtccgga caagatcaac
     3121 acggcgcaat ggttcgaggt gatggagacc gcccacagcg ttggtctcaa gagcaccgcc
     3181 accatcatgt tcggtcacgt cgatggctat cgccactggg cgcggcatct cattggggtg
     3241 cgccgcttgc aggcaaagac caagggcttt accgagttcg tgcccctgcc gttcgtacat
     3301 atggaagcgc cgatctatct caagggccag gcgcgcaaag gcccgacgct ccgcgaggcc
     3361 cttttaatgc acgccgtggc gcggctcgcg ctctatccgg agatcgtcaa tattcaggcg
     3421 tcctgggtga agatgggacc cgagggtgcc gtcgcctgcc tcgcggcggg ggccaatgat
     3481 cttggcggtg tgctgatgaa cgaatcgatc acgcgcgccg ccggggccat gcacggccag
     3541 gagatcggcg cgtccgacct tgcagccatg atccgtgcgg taggccgtag gcccgagcag
     3601 cgcaacacgc tttatgcggt gctgagaggc gacagcggcg gcggtccggg ccggcaatct
     3661 ccgcccctcg aagccgccga gtaggcgccg gcggagccct gcccatgacc cccttcgact
     3721 ttccgcgccg cgttgaggat gcaagcgtcc gcgcccgcgc ggttggcctt ttgaaggcgc
     3781 gcggggtgcg gctgccgacc tttgccgaac tggccgatcc gcaccccctg ccggccgccg
     3841 cccgcggcgc ccttgccagc gtcgacccgg atcaacccga cgccgccaat ctcgcgcgcg
     3901 tcaattggtt taatgactgg gatcgccgca cgcgccgcgc cacgccggcc tacctggaaa
     3961 tcccgcgagc gctgagcggg gttgaggcgc ccattctcgt cgccatcggc gatctcttcc
     4021 cgatgatttc ggcgcacaaa gtgttggccg cctacgcctg tctcgccccc cggctggcgg
     4081 ccgggcgttt cgatccgacc cgccagcggg cggtgtggcc ctcgacgggc aattactgcc
     4141 gcggcggcgt cgccatctcc cgtatactcg gctgccgcgg cgtggccgtg ctgccggccg
     4201 gcatgagcca ggagcgcttc gactggctcg ggcaatgggt gagcgccccg gaggacatca
     4261 ttcgcacgcc gggcacggaa tcgaacgtca aggaaatcta tgatgcctgc gccgagctgg
     4321 cgcgcgatcc gagccttgag atcatcaatc aattcagcga gttcgccaac tacctcgtgc
     4381 attggcgctg caccggcccg gccttggaga agattttcga gacccacgcc caggcttcga
     4441 cgctgacgct cgcgggcttc gtcgccgcca gcggctcggc cggcacgctc gcggccggcg
     4501 attatctcaa gcatcggcat ggcgccaaga ttgcggtggt ggaagccttg gagtgcccga
     4561 cgctcgccag caacggctat ggcgaacaca acatccaagg tatcggcgac aagcacgtgc
     4621 ccctgatcca caacgtcatg aacaccgacc tcgtcatcgg cgtgagtgac cgggcgacgg
     4681 atgcgcttaa cgcggtcttc aatagcgcgg ccgggcgcgc ccatttgctg cagcgcttcg
     4741 acctcagccc gcaattcgcc gaacacctca gccacttcgg cttgtcgtct attgccaaca
     4801 tcctgggcgc catcaaaatg gccaagcacc tggcgctcgg gccagattct gcgatcctca
     4861 ccgttgccac cgacggccat ggcctctacg gcagcgagct caagcggttt ctggcgcggc
     4921 ggcacaatag tgatgtcttg agcgaaaggt tcgccgccga gctgaccggc cagtacctga
     4981 tgggtaccgg cagcgaaaac gtactcgagg ccaccacgcg ggagcggcag cgtatgttca
     5041 acctcggtta cttcacctgg gtcgagcagc agggcatcgc cgttgccgat tttgatcgac
     5101 gccgacagtc gcagttttgg caggacttgc agggcttgct gccgctgtgg gatgagatga
     5161 tcgcggccgt caattgcgac agcggccagg tggcggcatg aggcgaggag ggcgttgcgc
     5221 atgagttcta cggcgcaggt tgcgccctat tcagagctgc ggcgggccac cttggcgcgc
     5281 gaccgaggtc tacgcgagaa ggtgatgtca ctcgaggcgg ccgcgcaact cgtgcgcgac
     5341 ggcgagcacg tggcgatcgg cggctgcacg ctgtcgcgca cgccgatggc catgatttgg
     5401 gccctgatcc gcgcgcagaa aaagaacctt gtgatctcgc gctcgatcac ctccaccgag
     5461 ggggatttgc tgctggcttc gggcgtgtct aagcacgtga tcaccagctg gttctcgcaa
     5521 ggcatcgtct ggggcgtgtc gaagatcatg cgccattaca cggagcgcaa gctcgcgcgc
     5581 ttcgaggagt ggagccacat gtcgatgggt ctgcgttacc gcgccggtgc catgggcatt
     5641 cccttcattc cctcacgctc gatgttgggg tcggacgtgg gtgagcgcgt tgtggagcag
     5701 atgcgcagct tgacttgccc attcaccggc gagcagttga cgctgctacc ggccctcaac
     5761 ccggattggg cgctgattca cgtgcaacgc tgcgacgcct tcggcaatgc gcagctcgat
     5821 ggcctgcagt ttatggacat cgatatcgcc atggcggcaa aacgtgtgat cctgacgacg
     5881 gagcgcatca tctcaaatga acagattcgc cgcgttcccg atttgacgcg gatcccattc
     5941 tttgccgtgg aagcggtggt cgaggtgccg attggctgcg cgccgcacga gtgctacggc
     6001 gtctatgagc ccttctttgg cgcacttgac gactatgccc gccgcgtggc gcgggctccc
     6061 gtgagcggct gtcgcccgta tctggaggag tactatttct cagccaagtc ctggccggat
     6121 tatttgagca agctcggcct ggatgcgatg ctcgatgcct gccgccgcgg ccggagcgtt
     6181 cacaatgact gaagaggctc agccgccgcc gcactacacc gcctccgagt tgttggccgt
     6241 gatggcaagc cgcctgttgc gggatgggca gatcgtgttt gccggcgtgg gcgtgccgct
     6301 gctggccgct acgctcgccc agcgcatcca tgcgccgcat ctgacgatcc tgttcgaggg
     6361 cggcacgatc ggtccgttca tcgtgcccgg ccagttgccc ccctctacca acgaacagcg
     6421 ttgcacgcga cgcgccaaca tggtgctgcc gatcaccgat gttctcctgt tgctgcagcg
     6481 cggctatgtg gacgtcggct tcatgggcgg tgcgcagatc gataaattcg gcaatctcaa
     6541 ttcctccttc atcggcgatg ccgacgaccc ggagatcagg ctgccgggca gtggcggcgg
     6601 caacgacatt tcctccttga ccgacatgat cgtggcgatg aagcacgaaa agcggcgctt
     6661 cgtggaaaag gtcgatttca tcacttcgcc gggctggctc gccgggcagc gcacgcgcgc
     6721 cgagcgcggc ctgccacaag gcggcatgtg gcgtgtggtc accgacctcg ccaccatggg
     6781 cttcgatacg gacagccgtg agatgaaggt cttaggcctc aatcccggcg tcagtcgcga
     6841 gcaggttgtg gacaacaccg gcttcaagct gatgtttgct gagaacctag agcccgtcga
     6901 gccgccgcgc caacgcgagc tggcggtgct gcgcgagctc gatccggaac ggctctacac
     6961 ggcgtgagga catggcggtg cagtcaagcg agaccctgcg caacaacgtg aaagaaaagc
     7021 ttgcgcgcaa cgagctggta tcgtcgataa cggtgcggct ggtgcgctct attgaaatcg
     7081 tgcgcatcgc caaaaccgcg ggcttcgatt cgctctacgt cgacctggag cattccagcc
     7141 ttggcctcga cgccaccggc cagatttgcc tggccgccct ggcgattggc atcgcgccct
     7201 tcgtgcgcgt gccggccaat tcgccggagt acatctctcg tgtgctcgac ggcggtgcgc
     7261 tgggtgtgat cgccccgggg gtccgctcgg ccgccgaggc gcgccacgtg gtagcggctg
     7321 ccaagtacgc tcctctgggt cggcgcggag cctccggcgc cctgccgcat ctcaactatg
     7381 ccaacctgcc ggccgatgag gccaatgcgg ccatgaacgc tgcgaccatg gtaatggtgc
     7441 agttcgagag cgccgaggcc atcgacaggg cggaggagat cgccgccgtc gacggggtcg
     7501 atctcatcat gatcggcacc aacgacttcc tggccgatat cggtctgccc ggccaattcg
     7561 aaagtccgcg cgtcacggac gcctatgcgc gcaccattgc ggcgtgccgc gcgcacggca
     7621 agcacgtcgg tgtcggcggc ttgacttcgc gtccccagcg catcgccgag ttcgtcaaga
     7681 tgggggcccg ttatgtttca accggcacgg acctagcctt tctgatggcc gaggcgagcg
     7741 cccgcgcacg acaggtgcgc gcgctctccc tgtgagggcc gccgcaagca aagaagcacg
     7801 cggcgtgcgg ccgccgacac cgcgaggtga tcaccgccgt cgcggtcaag atcgcgcgat
     7861 ggccatggct atggcattgg ccatgggtat gaggggtcag cgatgaagag cagccaggaa
     7921 ttcgtgcgcg tcgcagcggc cgaggcggag gccttcgggc aacgcctttt gcagcaccac
     7981 ggtgtgccgc ccgaggaagc agccattgtc gcgcactgcc tcgtgcgcgc cgatttgcgc
     8041 ggcatcgaca cgcatggcct acaatttctg ccgcagtatc tggcacgcgt tcggcgcggt
     8101 ttgatcaacc cccgtccggt actcgagccg cgccgggtga cgcctgtcgc cgccagcctg
     8161 gatggcgaca acggctttgg cttcgtggtc ggcatgcgcg ccatgcgcga agccatggag
     8221 atggcgcgcg gcttcggcat cggcatcgtc tccgcccacc gcagcacgca ttttggcatg
     8281 gccgccgcct atgtgctgcc ggcgctcgag gccggcttca tcgccatggt tttttccaat
     8341 gcctcgcggg ccatgccgcc ttggggtggg cgagaggccc tgctcggcac caatcccttg
     8401 gccgcgggcg ctccggcggg cagccaggtc ccctttctgc tggacatgtc gccagcggtg
     8461 gcggcgcgcg gcaagattcg ccgcgccgcg cggcgcggcg aggccattcc gccgggttat
     8521 gcgcttgacg cggacggccg acccaccacc gatcctgcgg cggcgctcga cgggggcgtg
     8581 gtgctgccga tcggcggcta caagggttcg ggcctgtcga tgctgatgga catcatgggc
     8641 ggggtgatct cgggagcggc ctgcgcgggc gacgtggcga gccagttcct cgactacgat
     8701 cgcccgcaga acgtcggaca cttctttctc gccctcaagc ctgacctgtt tgtcgaaatc
     8761 gaggactatc gcgcgcgcat ggatcgcttg gttgagcgcg tgcatcagag cccgaccgcg
     8821 catggcttca cggaggtggt gatgccgggc gaactcgagg cgcggctcga agccgagcgc
     8881 cgcagccagg gcgtgcctta ccatcgcaag gagttcgcgg cattggcggc ggaggcggcc
     8941 agcgccggcc tgccgccctt gccggtgctg caggcggagt gaatggcatg cgggtcggcg
     9001 gttatgacat cgacgtcatt gtgcaaggct accccggcaa ggccgtgtgc cacggcggtc
     9061 tcggctggag cagcgtggtg ttgctcagag gccagggacg tattgccctg atcgatgccg
     9121 gctcgtttgg tttgcgcccg ttgctgctgc cgaggctcaa ggcgcgcgct ttggcacccg
     9181 gcgatgtcac cgacctgctg ctgacccatt cccatcacga tcacgccgtg aactggacgc
     9241 tctttcggca tgcccgcatt cgcatcggcg cgcacgagct tgcctggtcg gtcaaggagc
     9301 cgtggggcga gacgccggtc ccggaacttt acatgcgcga acttgagacc tggccgacgc
     9361 tggcaacgat tcaagatggt gaggaggtat tgccgggtat tagggcgcac atggcacccg
     9421 gccacacgcc tggccatctg atctttgtgc ttgagggccg cgagcatgac gtgattttca
     9481 ccggcgatgc cgccaagaac cgcgccgaac tcatctcgcg cacggccgac atgacctatg
     9541 acccacgcgt gacggaaaag accattgaaa tgatctggtc gttctggcgg cggcggcctg
     9601 gcagcatcgt cgtcccgggc cacgacctgc cgttggcgca ggagaacggc cggccgcgct
     9661 acatcggcaa gcgcgaagct gcggtcaccg cctggttcgg cgatgacatg gagcgcacga
     9721 cgctcttcaa cctgaccgtg gcctaacagg cgacctgccc aggagctggg cgcgccctgc
     9781 ccattaaaga ggattgtcag aaaccgttgc gaccccctag gatcgccacg ctcggatccc
     9841 gcgcaattgc agaaggagtg cgccatgcgc ttgcggaatg tcgtcgttgc cgctctcctc
     9901 gtgccgttgc tgccgggaat cggcacggcg cagcagccgc tcaagaccgc cgtcgacggc
     9961 acctttgcgc cgcatgccat gccgaagctc gaaggcggca ttcagggctt caatgtcgat
    10021 cttgccaacg aggtgggcaa gcggctcaag cgcccggtcg agattcaggc catgcagttc
    10081 tccggcatcc tgcccgcctt gcaagccggc accctcgact tcgttgccgc gcccgtgacg
    10141 gtgaccaagg agcgcgccga caattacatt tttaccgagg gttatctcaa cacggactat
    10201 cagttcttga ccaaaaaggg cgagccgccg atcaccgatc tcaagcagtt caagggcaag
    10261 gtgattgccg tcaacaaggg ggcggtctac gacagttggg cacgaggcct ggaagccgag
    10321 atcggctggt ccgtggagag tttcggcacg cagaccgatg ccgtgcaggc cgtgctcgcg
    10381 gggcgggcct atgccaacgt ggcgggcaac accgcaatac agtgggcagt caagcagcaa
    10441 ccgcagttgc aactgtccta tctgcattcc accggactgg tctgggccat gccgctgcgc
    10501 aaggattcgc tggcgctgcg gacagagctc gagaacgcca tcgaatgcat gaaaaaggat
    10561 ggcacgatgg caaccatgca cgagcgctgg tttggcacca agcccgaggc gaactcgccg
    10621 gcggttacca tctatccagg cacgggcgtt ccgggaacgc aagggtggga ccagaccccg
    10681 cgcgagccga agtgtgactg aggcgggcag gaggcgcgct gcccgcgcgc cggcgcaagg
    10741 gtgatgctcg ggcatggcga tattgacggt ctcgggcctt agaaaaagct tcggggccaa
    10801 tgaggtgctg aggggtatcg agctcagcgt cgccgagcgc gaattggtct tcatcatcgg
    10861 accgtccggg tcaggcaaga gcacgttact gcgctgcctc aaccgcttgg aagagccgac
    10921 cgccgggagc attaccttcg atggccagga ggtgaccgcg cgcgccacca acctcaatcg
    10981 gttgcgccgg tccatgggta tggtgttcca gtccttcaat ctctacccgc acatgaccgc
    11041 gctcggcaat gtgaccttgg ccttgcgtcg tgtgctcaag aaatcgcggg cggaggccga
    11101 tgctctcggt ttggccgcgc tgaccggcgt cggcctggcg gacaaggcgt ccagttatcc
    11161 caacgagctc tcgggcggcc agcaacagcg cgtagcgatc gcgcgtgccc tggcgcttga
    11221 tccaaggatc gtgctgttcg acgagccgac ttcggcgctc gatcccgagc tggtgggctc
    11281 cgtgctcagc gtcatgcgcg acctcaaggc gcgcggcatg accatgctgg tggtgagcca
    11341 cgaactgcgt ttcgcccggg aggcagccga ccgcgtcgtt ttcatggatg cgggccgcat
    11401 tgttgaggag ggagcacccg agacgatctt cagtgccccg cgcgagatgc gaaccaaggc
    11461 attcgttgcc cagctgatcc ggtgatccca tgacggcatg gcagcgcttc ctggaaacct
    11521 ttttcaagcc ggacatgctc gcccagtact ggcccagcat cgcgcatggc gtgctggtga
    11581 cgctgcagat tgcctctgcc gtcgtgctca ccggcctctc ttttggcctc ctgctcgccg
    11641 gcctgcgcgc ctaccgtctc ttgcctctca atatcgccat tgtcgtcttc gtcgacgtgt
    11701 tccgctcgct gccgccgctg gtggcaatcc tgattatcta cttcggactt cccggggtgg
    11761 ggatcaactt gccgagcttt gttgtgctgt ggctcgtgct gtcgttggtg ctcaccgcct
    11821 tcgccgagga gattttttgg gccggcatca tttcgacccc caagggacaa tgggaagcgg
    11881 cgcgttcgac cggccttggc ttttccgaga cgctcctatt cgtggtgctg ccgcaggccg
    11941 ttcgcctcgg cgtgccgccg ctcaccaacc gtaccatcgc cattaccaag aacacggcgc
    12001 tcggcaccgt gatcggcgtc ggcgagatct tgaaccaagc gacggcggcg cagtcgtttt
    12061 cgggcaacgc cacgcccctg atgatggggg cgattgccta cattttattg ttcctcccgg
    12121 tggttgccgt cggtcgctgg atcgagacgc ggtttcagtg gcggagggct tgatgcaaga
    12181 gatcggtcag cagttcctca atgtcgagat catgctgaag atctggccgc tcctgatgcg
    12241 cggttttgcc accacgctct atctctgcgc gctgacgatc ccgctgggat taatcggcgg
    12301 gcttggcgtc gcgctgatct tgctcagtcg cagccgcctc acgcgctggc ttgtcatcgc
    12361 tttggttgat ctcttccgcg ccgccccgcc gctagtactg ctgatcctca tctattccgg
    12421 cctgcccttc gcgggaatta ggctttcgcc gacggcggcg gtgtgtctag ccttcttttg
    12481 caacacctcg agctactatg gggaaatcta ccgcgcgggc atcgagagca tcggccgcgg
    12541 gcaatgggag gccgcgcgct cgacggggct caacggcttc gagaccttga ccttcgtcgt
    12601 gctgccgcag gccgtgcgca acgtgctgcc ggacctcgtt tccaatacgg tcgaggtcgt
    12661 caagctcacc tccatcgcca gcgtggtgtc ttttccggag ttgctatact ccgccgatat
    12721 ggcacgctcc gtcaccttca attccagccc tgtcgtgctg gccgccatcg tttatcttct
    12781 gttgctgtgg cccctggttc ggctgataag ccggctcgag cgcggggttg cgacgcacta
    12841 aagggagcaa gaggaagccg catgtcccgc atcgtcaccg ttgctgcggc ccagctgggc
    12901 gccattcaga aggcggagcc gcgccaggcg gtcatcgctc gaatgctcgc tctgatggat
    12961 gaggccaagg ccaagggcgc cgatctcgtg gtctatccgg agctggcgct gaccacgttc
    13021 tttccgcgct ggtattatga ggatcgctcc gaggccgacc tttggttcga gcgggagatg
    13081 cccaacgccg ccgcgcgtcc cttgttcgag cgcgcgcaaa agcatggcct ggggatgagc
    13141 ttcggatatg ccgagctgac agcggatggg catcacttca acacggcgat cctggtggac
    13201 aagagcgggg cgatcgtcgg taaatatcgc aaggtgcatc tgcccgggca cgccgagttc
    13261 gacgctcggc gcacccacca acacctggag aaacgctatt tcgagcccgg ggatctgggc
    13321 tttccggtgt ggcgctgctt gggcggcatc ttcggcatgt gcatttgcaa cgaccggcgt
    13381 tggccagaga cctaccgcgt catgggcctg cagggcgtgg aaatgatcgt cctcggcttc
    13441 aacacgccgt ccgtcaattc tcagaagggt gacgagggac cacaaaagcg catcttccac
    13501 cacaagttat ccgtgcaggc cggtgcctat cagaactcga cctgggtggt ggcggtggcc
    13561 aaggccggtg ttgaggatgg gcatcccctg ttcggttgca gcctgatcgt caatccggat
    13621 ggcgaaatcg tcgccgagag caagacggaa gcggacgagg tcatcgtgca tgcctgcgac
    13681 ctcgatgcca cccgcttcgg caaggagacg atcttcgact tcgaccgaca ccgccgcatc
    13741 gagcattacg ggcgcattac ggcgcaggcg ggcgccgaac tgccaaagga gaggtgagtg
    13801 ggacatctga gctacaattt tgcggagttg cagccgagtc agcgctacaa gctcctgtgc
    13861 ggcctggtgg tgccccgccc gatcgctttg gtaacctcga tcagccgcga gggtatcgtc
    13921 aatgccgcgc cgttctcgtt ctttaacgta ttctccgaga acccagccct gctggtcttg
    13981 ggtttgcagc acaacgagga tggctcgccg aaggacacga cccgcaatat ccatcgcagc
    14041 ggcgagttc
//
LOCUS       DASTMT010000384         1638 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_869423, whole genome shotgun sequence.
ACCESSION   DASTMT010000384 DASTMT010000000
VERSION     DASTMT010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 1638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1638
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_869423"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1638)
                     /locus_tag="VFR00_09035"
     CDS             complement(<1..>1638)
                     /locus_tag="VFR00_09035"
                     /inference="COORDINATES: protein motif:HMM:NF012618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0159447.1"
                     /translation="SHDKTVRLWRLPEGRLLRTLRVPAGPGNDGKIYSVAMAPDGSWV
                     AAGGWDTAARTQRVNFVYIFDTATGAVTARLGPFANVINHLAVSVDGTYLAVLVGSEG
                     VRVWERTSADGRQWQVVATDRDYGGKMAYGAAFDRRGTLYTVGYDRKLRRYPAPYGGK
                     PSAVLTKGGSEPHSVEVHPSGDRVAVGFVDAAAVDIYDAQSLAWRAAANVKGVDNGNV
                     GSVTWSADGTRLYAAGRYVKSGKIAIRVWDRAGLGAPRDLTGPLTTIMHVLPCGDGIV
                     FGAAEPAFGVLDSKGNPRTWQDSVQADMRGKRFEHFTVSADGRRLRFGFKEYGVEPVL
                     FDLGTATLTDAANAVPDLSPADTQGLPVTDWINEARPKFAGAPLALEPYEIARALAIA
                     PNKQRFVLGTEYWLRAYDAQGKPAWQTPVPAVAWGANIPREARVVIVAYGDGTIRWHR
                     LDDGQELLALFVHAKDRRWVAWTPRGYYTASPGGEDLIGWQLNRNWNDAADFFSVARF
                     RDQFYRPDIVRLVLSTLDEAKAVEQANQSSKRNRDTE"
BASE COUNT          263 a          539 c          573 g          263 t
ORIGIN      
        1 tcctccgtat cccgattgcg tttggaagac tggttggctt gctcgacggc cttggcctca
       61 tcgagcgtgc tgagcacgag ccggacgatg tcggggcgat agaattgatc gcgaaagcgg
      121 gcgacggaga agaaatccgc cgcgtcgttc cagttgcggt tcaattgcca gccgatcaag
      181 tcctcgccgc cgggcgaggc ggtgtaatag ccgcggggcg tccaggccac ccagcgccga
      241 tccttggcat gcacgaacaa ggccagcaat tcctggccgt catcgaggcg gtgccagcgg
      301 atggtgccat cgccgtaggc aacgatcacg accctggcct cgcgagggat gttggcgccc
      361 caagccacgg ccggtaccgg cgtctgccag gcgggcttgc cctgggcgtc gtaagccctg
      421 agccaatact ccgtacccag cacgaagcgc tgcttgttgg gtgcaatcgc cagcgcgcgg
      481 gcgatctcgt agggctcgag ggccaagggc gcgcccgcga atttggggcg ggcctcgttg
      541 atccaatcgg taaccggcaa gccttgcgtg tccgccgggc tgagatccgg cacggcattg
      601 gcggcatccg tcagcgtcgc cgtgccgaga tcgaacagga ccggctcgac gccgtactcc
      661 ttgaagccga agcggaggcg ccggccgtcc gccgagaccg tgaaatgctc gaagcgcttg
      721 ccgcgcatgt cggcttgcac gctgtcttgc caggtgcgcg gattgccctt gctgtcgagc
      781 acgccgaagg ctggctcggc ggcgccgaac acgatgccat cgccgcaggg cagtacatgc
      841 atgatggtgg tcagggggcc ggtcaggtcg cgcggggcgc cgaggccggc gcgatcccag
      901 acgcgaatgg cgatcttgcc ggacttgaca tagcgtccgg cggcgtaaag gcgcgtgccg
      961 tccgccgacc aggtgacgct gccaacgttg ccgttgtcga cgcctttgac attcgccgcc
     1021 gcgcgccagg ccagcgattg ggcgtcgtag atgtccacgg cggcggcatc gacgaagccg
     1081 acggctaccc gatcgccgga gggatgcacc tcgacggagt gcggttcact gccgcctttt
     1141 gtcagcaccg cgctgggttt gccgccgtag ggggcaggat aacgccgcag cttgcgatca
     1201 tagccgaccg tatagagggt cccgcgccgg tcgaaggcgg cgccgtaggc catcttgccg
     1261 ccgtagtcgc ggtcggttgc caccacctgc cactgccgcc cgtcggcgct ggtgcgctcc
     1321 cacacgcgca cgccctcgct gccgaccagg acggcaaggt aggtgccatc gacggagacg
     1381 gccagatggt tgatgacgtt ggcgaacggc ccgaggcggg cggtgacggc gcccgtggcg
     1441 gtatcgaaga tataaacgaa gttgacgcgt tgggtccgcg ccgccgtatc ccagccgccc
     1501 gccgccaccc agctgccatc aggggccatc gccaccgaat agatcttgcc gtcgttgccg
     1561 gggccggccg ggacgcgcag cgtgcgcagg agccgtcctt ccggcaagcg ccacagccgc
     1621 acggtcttgt cgtgcgag
//
LOCUS       DASTMT010000385         1786 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_2504800, whole genome shotgun sequence.
ACCESSION   DASTMT010000385 DASTMT010000000
VERSION     DASTMT010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 1786)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1786)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1786
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2504800"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..905)
                     /locus_tag="VFR00_09040"
     CDS             complement(<1..905)
                     /locus_tag="VFR00_09040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015684121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU0159448.1"
                     /translation="MQVGEVAQVSMGQTAPTNTSDPNYFHKVVDCQWACPTHTPVPEY
                     IRLIAQKRYAAAYMLNWESNVFPGILGRVCDRPCEPACRRGRVEEKPVAICRLKRVAA
                     DYKGEIDAFMPDVPSEKNGKRIALIGAGPASLTVARDLLPLGYTCTLFEKDARGGGLM
                     RTNIPSFRLPETVLDEEIDRILGFGVEARFGSEIKSLEALLGQGFDAVFIGTGAPKGK
                     DLEIPGRRQGASNVHIGIDWLTSVAFEHVNRIGRRVVVIGGGNTAMDCCRTALRLGGE
                     DVKVTVRSPRRVMKASPWEIEDAEH"
     gene            1248..>1786
                     /locus_tag="VFR00_09045"
     CDS             1248..>1786
                     /locus_tag="VFR00_09045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010683329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HEU0159449.1"
                     /translation="MQQRLASSLRQLKSHYDAIVIGSGYGGGVAAARLARAGKRVAVL
                     ERGREIPVGAFPRRFSDLRNDLQLSSRQTQLGSPTGLYDVRLGDDMHILVGCGLGGGS
                     LINAGVALRPDARVFSDPAWPGELARDPLLDAGYLEAQRWLRPARDAAAHERSKYQAL
                     GAASVALGVAPVAAPVTVS"
BASE COUNT          272 a          601 c          574 g          339 t
ORIGIN      
        1 tcgtgctcgg cgtcctcgat ctcccacggc gaggccttca tcacccgccg cggcgaacgc
       61 acggtcactt tgacgtcctc gccgccgagc cgcagcgcgg tacggcagca gtccatggcg
      121 gtattgccgc cgccaataac cacgacgcgc ctgccgatcc ggttgacgtg ctcgaaggcg
      181 accgaggtga gccaatcgat gccgatatga acgttgctgg ccccttgcct gcgcccgggg
      241 atctcgaggt ccttgccctt gggggcgccg gtgccgatga aaacggcatc gaagccctgg
      301 cccagcaagg cctcgaggct cttgatctcg ctgccgaagc gcgcctccac gccgaacccc
      361 aaaatgcggt cgatctcctc gtcgaggacg gtttcgggta ggcggaagga ggggatgttg
      421 gtgcgcatca agccgccacc gcgcgcatcc ttttcgaata gcgtgcaggt gtagccgagg
      481 ggcagcagat cacgcgccac cgttaaagag gccggcccgg caccaatcag ggctatgcgt
      541 ttgccgttct tctcgctggg cacatccggc atgaaggcgt cgatctcgcc cttgtagtcg
      601 gcggccacgc gcttgaggcg gcaaatggcg accggctttt cctccacgcg gccgcgtcgg
      661 cacgccggct cgcagggccg atcgcacacg cgcccgagaa tgcccggaaa gacattgctc
      721 tcccagttca gcatgtaggc cgccgcgtag cgcttttgcg cgatcaggcg aatgtactcg
      781 ggcaccgggg tgtgagtcgg gcaggcccac tggcaatcga cgaccttgtg gaagtagttc
      841 ggatcggagg tgttggttgg ggccgtttgg cccatgctta cctgagcgac ctcgcccacc
      901 tgcatttgcg ttcctgcccc tattttcgcc tcgccccaac cgcctcttct acaagaagag
      961 accccgttgt cccaacagca atgatgggac gaaatgggat ttttcaaccg acccacggcg
     1021 ccttctggcg catctagcac gcccgttcgc gatggtcatt gtgcactgca aaatgtcctc
     1081 tcgccggcgc taagggaacc gggctcccac ggccgcgttc tcgctcggga gcgcgctgat
     1141 ggtcgcgaag ctgttggtaa ttgcgttgcc cgcctgggtc acggcttgcc atgcgggagg
     1201 cgcagcggct aggcgtcgct ctgactttga ccatgcctcg gatgcctatg cagcaacgac
     1261 ttgcctcctc cctgcgccag ctcaagtccc actacgacgc catcgtcatt ggctctggct
     1321 atggtggggg tgtcgcggct gcgcgcctgg cgcgggcggg caaacgcgtt gccgtcctgg
     1381 agcgcggccg cgaaattccc gttggcgcct ttccacgccg gttctccgac ctgcgcaacg
     1441 atctgcaact ttcgagccgg cagacgcagt tgggctcgcc gacgggcctt tacgacgtgc
     1501 ggcttggcga tgacatgcac atcctggtcg gctgcggcct cggtggcgga tcgctgatca
     1561 atgcgggcgt ggctctgcgc cccgatgcac gcgtctttag cgacccggcc tggcccggcg
     1621 agcttgctcg cgatccgctg ttggacgcgg gctatctcga ggcccagcgg tggctcaggc
     1681 cggcgcgtga tgccgccgcc catgagcgca gcaaatatca ggccctcggc gccgccagtg
     1741 tcgccctcgg cgtggctccc gtcgcggccc cggtcacggt gagctt
//
LOCUS       DASTMT010000386         2540 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_5032626, whole genome shotgun sequence.
ACCESSION   DASTMT010000386 DASTMT010000000
VERSION     DASTMT010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2540)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2540
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5032626"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            33..1025
                     /gene="secF"
                     /locus_tag="VFR00_09050"
     CDS             33..1025
                     /gene="secF"
                     /locus_tag="VFR00_09050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HEU0159450.1"
                     /translation="MRLIPNFKGFDFFPHDTRLPFMRYKGLCFGLSIAAMLISLAIIG
                     VRGFNYGVDFKGGSLIEVQSTSGPADVAQLRERLNKLGVGAVQIQSFGGPTDVLIRVE
                     QQPGGEGEQQAALKKVQEALGTRYAQRRVEVVGPAVSGELRMTGFIAVAAGLLAIVAY
                     VWFRFEWQFALGAIVALTHDVLVTAGIFSLFQLEFDLSIVAALLTILGYSVNDTVVVS
                     DRIRENLRKFKRMDLNELLNISINETLSRTILTGCTAIAVLVALYAFGGEVIRNFNLA
                     MLLGVVIGTYSSIFIAAPLLGYLGVRRDWSGGPAAKPGKSQAATSSQRLNVASK"
     gene            1022..1399
                     /locus_tag="VFR00_09055"
     CDS             1022..1399
                     /locus_tag="VFR00_09055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006316306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH938/NDUFAF3 family protein"
                     /protein_id="HEU0159451.1"
                     /translation="MSAPPACYPGRAAIDAYGNGGFRFAGMSHRGSILCLPSGIYAWA
                     PARPEDISLESLAPALTEGDGLDLLLLGSGRVGVMPAWELRQSFADLGVALESMDTGA
                     ACRTYNVLLAEARPIAAALIAVN"
     gene            1411..2013
                     /locus_tag="VFR00_09060"
     CDS             1411..2013
                     /locus_tag="VFR00_09060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0159452.1"
                     /translation="MSGIIDNPRSCLLAGAMLATVLLVVALSVGETDRLGLFAFLVRL
                     IHVLSAMLWVGFVFFVNFVQLVALKAADETGRGVIHTAIVPRVAWWFRHASTLTVISG
                     AVLLLFAGYLLPTLIYGTSVYIPAGKAIVLWAAVLGALAMWMFVHMYIWPNMQIVLGL
                     RSGDAAAKTEARNRVILFARLNLILVVPVVLGMIAAAHLY"
     gene            2018..>2540
                     /locus_tag="VFR00_09065"
     CDS             2018..>2540
                     /locus_tag="VFR00_09065"
                     /inference="COORDINATES: protein motif:HMM:NF012705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene/phytoene synthase family protein"
                     /protein_id="HEU0159453.1"
                     /translation="MREDRSLEPIHDTIIASARAGEPDRYLAALLAGRAARPDLLALA
                     AFAAELARIGVAVRREPAMAEIRWQWWREALGRAGGAETGHPIADQLRALLRRHGIAP
                     AALIGLIDAHAHDLDVLPFADDTELDRHLLATEGALFQLSARVLGLANGSGPAWSDWA
                     GRAYGMARLLFGLG"
BASE COUNT          353 a          842 c          834 g          511 t
ORIGIN      
        1 ccgaggtccc ggtctaggaa aatagggcga ccatgcggct catccccaac ttcaagggct
       61 tcgatttctt cccgcatgac acgcggctgc cgttcatgcg ctacaagggg ctctgctttg
      121 ggctatcgat cgcggcgatg ctgatctcgc tcgccatcat cggggttcgg ggtttcaact
      181 atggcgtcga tttcaagggc ggctcgctga ttgaggtgca gtcgacctcc ggcccggccg
      241 acgtggcgca gctgcgcgag cgcctgaaca agctcggggt cggcgcggtc caaatccaaa
      301 gcttcggtgg ccctaccgat gtcctgatcc gcgtcgagca gcagccgggg ggcgaaggcg
      361 agcaacaggc tgccctcaag aaggtgcaag aggccctggg tacgcgctat gcgcagcgcc
      421 gggtcgaggt ggtcggcccg gcggtgtcgg gcgaactgcg catgacgggc ttcatcgccg
      481 tcgccgccgg gctgttggca atcgtggcct acgtttggtt ccgcttcgaa tggcagtttg
      541 ccttgggcgc catcgtggcg ctgacccacg acgtcttggt gaccgccggc attttctcgc
      601 tcttccagct cgagtttgat ctttccatcg ttgccgcgct cctcaccatc cttggctatt
      661 cggtcaatga cacggtcgtc gtctccgacc gcattcgcga gaatttgcgc aagttcaagc
      721 gcatggacct caacgagctc ctcaacattt cgatcaacga gaccttgtcg cgcacgattc
      781 tgacgggctg tacggccatt gccgtgctgg tcgcgctcta cgccttcggc ggtgaagtga
      841 tccgcaactt caaccttgcc atgctgctgg gcgtggtgat cggcacttat tcgtcgatct
      901 tcatcgccgc gccgctgttg ggctacctcg gggtgcgccg cgactggagc gggggccccg
      961 ccgccaagcc cggcaaaagc caggccgcga ccagctcgca acgcctcaat gtagccagca
     1021 aatgagcgcg ccacccgctt gctatccggg gcgggccgcg atcgacgcct atgggaatgg
     1081 cggtttccgc ttcgccggca tgagccaccg cggctccatc ctgtgtttgc cgagcggcat
     1141 ctacgcctgg gcgccggcca ggcccgaaga tatttcgctt gagagcctgg caccggcatt
     1201 gaccgaaggc gacggccttg atcttttgct gctcggcagc ggtcgggtgg gcgtcatgcc
     1261 ggcgtgggag ctgcggcaaa gctttgccga tctgggcgtt gccttggaaa gcatggatac
     1321 gggcgcggcc tgccgcacct acaacgtatt gttggccgag gcacggccga ttgctgccgc
     1381 gctgatcgcc gttaattgag gggccaggcg atgtcaggca tcatcgacaa tccacgctcc
     1441 tgcctgttgg ccggcgccat gctcgccacc gtgttgttgg tcgtggcgct gagcgttggc
     1501 gagacggatc gtctgggcct cttcgccttc ctcgtgcgcc tcatccatgt gctctccgcc
     1561 atgctctggg tgggttttgt cttcttcgtc aacttcgtgc agctcgtggc gctcaaggcc
     1621 gccgacgaga caggccgcgg cgtcatccac accgcgatcg tgccgcgggt cgcctggtgg
     1681 ttccggcacg cttcgaccct gaccgtgatc agcggtgccg tgctcttgct gtttgccggt
     1741 tatctgttgc cgaccctgat ctacggcacc agcgtttaca ttcccgccgg caaggcgatc
     1801 gtgctgtggg cggccgtgct cggggcgctg gccatgtgga tgttcgtgca tatgtacatt
     1861 tggcccaaca tgcagatcgt gctgggcctg aggtcgggcg atgccgcggc caagaccgag
     1921 gcacgcaacc gcgtcatctt gttcgcccgg ctcaacctca tcctcgtcgt gccggtcgtg
     1981 ctcggcatga tcgcggccgc gcacctctac tgacgtggtg cgcgaggatc gcagcctcga
     2041 gcccatccac gatacgatca tcgcctcggc ccgggccggg gagccggacc gctacctggc
     2101 ggccctgctg gccggccgcg ccgcgcgccc ggatttgctg gcgctcgccg ccttcgccgc
     2161 cgagctcgcc cgcatcgggg tggcggtgcg gcgggaaccg gcgatggcgg agatccgctg
     2221 gcagtggtgg cgcgaggcgc tcggacgtgc cggcggcgct gagaccgggc atcccatcgc
     2281 cgaccagctg cgcgccctgc tgcgccgcca cgggatcgcc ccggccgcgc ttatcggcct
     2341 catcgatgcg catgcgcacg acctcgatgt gctgcccttt gccgacgata ccgagctcga
     2401 tcgccatctc ctcgccaccg agggggcgct gttccaactt tcggcgcgcg tgctggggct
     2461 tgccaacggc tcaggtccag cctggagcga ctgggctggc cgcgcctacg gcatggcacg
     2521 gctactgttt ggcctcggcg
//
LOCUS       DASTMT010000387         8603 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_5687693, whole genome shotgun sequence.
ACCESSION   DASTMT010000387 DASTMT010000000
VERSION     DASTMT010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 8603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8603)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8603
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5687693"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1065
                     /gene="lepA"
                     /locus_tag="VFR00_09070"
     CDS             <1..1065
                     /gene="lepA"
                     /locus_tag="VFR00_09070"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015596601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HEU0159454.1"
                     /translation="QEMLAELGPGEVGFFTAAIKQVADTRVGDTVTDEKNPTATPLPG
                     FKPTQPVVFCGLFPVDAAQFEDLREAMARLRLNDASFSYETESSAALGFGFRCGFLGL
                     LHLEIITERLEREFNLELITTAPSVIYHVYCSDGTRLEMHNPADMPDPVQIDRIEEPW
                     IEATILVPDEHLGSVLKLCQDRRGRQKNLTYAGTRALLVYELPLNEVVFDFYDRLKSI
                     SRGYASFDYHLSGYEVGDLVKMSILVNAEPVDALSIIVHRARAESRGRAMCEKLKELI
                     PPHLFQIPIQAAIGGRIIARETIRALRKDVLAKCYGGDITRKRKLLEKQKKGKKRMRQ
                     FGEVEIPQEAFIAALKMEEN"
     gene            complement(1046..2137)
                     /locus_tag="VFR00_09075"
     CDS             complement(1046..2137)
                     /locus_tag="VFR00_09075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015952877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF817 domain-containing protein"
                     /protein_id="HEU0159455.1"
                     /translation="MGQRRQHERTARRTLHRGLAVAPTPDPSNRRGGVAPPLAGQGRL
                     ESPATPELPTLSGARHWAPLARLIAQEARIGDWAERRRHTLILYEFVRFGIKQGWASL
                     FGGLLLACLIGTHLFYPPAAGLARYDFLVLAAVAIQAVMLTAKLETWDEAKVIFVFHV
                     VGTAMEVFKTSVGSWIYPEASLLRLGGVPLFTGFMYAAIGSYIARCWRLFDFRFTHHP
                     PLGWVVLLGVGIYANFFAHHYVTDLRLGLFAVTAVLFGRCWIYFKVWRVWRRMPLLLG
                     FLLVALFIWVAENIGTFTAAWMYPHQRHGWALVPSSKLGAWFLLMIISYALVAMLNRP
                     QRPSEAALGARRAQGHLEAVTASLSSPPS"
     gene            2455..4620
                     /gene="scpA"
                     /locus_tag="VFR00_09080"
     CDS             2455..4620
                     /gene="scpA"
                     /locus_tag="VFR00_09080"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018260523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HEU0159456.1"
                     /translation="MGAIPDFTQVGFAAPPPVRRLGGGEPWLTPEGIAVKDAYAAADS
                     AGLDFLDGYPGIAPYLRGPYPTMYVTQPWTIRQYAGFSTAEDSNAFYRRNIAAGQKGL
                     SVAFDLPTHRGYDSDHPRVKGDVGMAGVAIDSIYDMRTLFDGISLADMTVSMTMNGAV
                     LPVLALFVVAAEEQGVPPQRLAGTIQNDILKEFMVRNTYIYPPEPSMRIISDIFAYTS
                     ANMPKFNSISISGYHMQEAGATADLELAYTLADGVEYVRAGLAAGLGIDAFAPRLSFF
                     WAIGMNYFMEIAKMRAARLLWAKLIKAAAKPKDPKSLALRTHCQTSGWSLTAQDVFNN
                     VTRTAIEALAATHGGTQSLHTNALDEAIALPTDFSARIARNTQLLIQQETGTCRVIDP
                     WGGSHYVEHLTYALLRRAQEHIAEVEGLGGMAKAIAAGIPKMRIEEAAARTQARIDAG
                     QQTIVGVNKYRVGDAAAIEVLKVDNKGVRDRQIAKLERLKAERDPAAVAATLAALTTT
                     AQSGEGNLLASAIAAARAKATVGEISEAMEKVWGRHRAEARAVSGVYTAEAGAMSKAV
                     ERVRSMCQDFEKNDGRRPRILVAKMGQDGHDRGQKVIASAFADLGFDVDIGPLFATPE
                     EAARQAVENDVHIIGVSSLAAGHLTLVPELTAALTKEGRGDIMIVVGGVIPPADYEAL
                     FQAGAQAVFGPGTNIPSAAADLLDKLNAARGYGPRAAAE"
     gene            complement(4729..4995)
                     /locus_tag="VFR00_09085"
     CDS             complement(4729..4995)
                     /locus_tag="VFR00_09085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0159457.1"
                     /translation="MLIHINDRQPSVERNVGGLHRIGQPILLVFHANDGCPYTPAAGA
                     NRPKKLLRGANRVDLVRLTAARPAAGSRVRRAHTMALQVRTKRW"
     gene            5637..7154
                     /gene="glpK"
                     /locus_tag="VFR00_09090"
     CDS             5637..7154
                     /gene="glpK"
                     /locus_tag="VFR00_09090"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009735106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HEU0159458.1"
                     /translation="MADRSAILALDQGTTSSRAILFGSDLSVLGAAQREFAQHFPAPG
                     WVEHEPEDIWRTTVETARQALAGPDRSPASIAGLGITNQRETCLLWDRRTSQPIHRAI
                     VWQDRRTAEVCGALKRQGHEPEITARTGLLLDPYFSASKIAWLLEHVEGARALAAAGH
                     LAFGTIDTFLIWRLTGGRVHATDATNASRTLLYDIGKGAFDDDLLRLFKVPRSILPDV
                     FDCNAEFGLTEPSILGAAVPICGVAGDQQAALIGQACFTPGMVKATYGTGCFAVLNTG
                     SEQVASHNRLLTTVAYQLGGQRTYALEGSIFIAGAAVQWLRDGLRLIASAAESGRLAG
                     AADPAQDVYLVPAFVGLGAPYWQPHARGALFGLTRATGPNEVARAALEAVCYQTRDLF
                     EAMQQDWRAGAGAVLRVDGGMVASDWTMQFLADVLDRPVERPTISETTALGAAWLAGH
                     KAGVWPDAEGFARQWKLERAFAPKLSASERERKYTGWRRSVAAVLEAAKDVRGEA"
     gene            7151..7762
                     /locus_tag="VFR00_09095"
     CDS             7151..7762
                     /locus_tag="VFR00_09095"
                     /inference="COORDINATES: protein motif:HMM:TIGR04025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEU0159459.1"
                     /translation="MTQIDNLEALRQVYAQPRDLALRKELDHIDRHSREFIGRAPFVV
                     IATADKDGWPDASPRGDAPGFVTVLDEKHLHLPDRPGNNRLDSFQNLLGNAKIALLFL
                     VPRRLETLRVNGTARLLTDPELCARHAVAGKPARAILEVTACSVYFQCGKALLRSALW
                     QLECWPPLDGLASFAEALADQIKGLDKVASQSRLEKAYRDTLY"
     gene            complement(7741..8535)
                     /locus_tag="VFR00_09100"
     CDS             complement(7741..8535)
                     /locus_tag="VFR00_09100"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU0159460.1"
                     /translation="MPFALIGQRRIAYRSIPGAPGRPPLVFLHEGLGSAELWRDFPDS
                     VAARLGARALIYSRFGYGQSDGLNGKRAPQFMHEEALTVLPALLDATGIERPLLIGHS
                     DGASIALIHAACGRRPLAGLVLMAPHSFVEPVCVGSIANIRATYCSTADDASGLRNRL
                     AKYHARVDDAFLGWADVWLAPEFQSWNIEDLTRAISTPILLIQGADDEYGTLAQLDSI
                     EARAQGPVSRLVLTNCGHAPHRDRARDVLEAIVAFARDEAQYSVSR"
BASE COUNT         1532 a         2792 c         2860 g         1419 t
ORIGIN      
        1 caggagatgc tggcggagct tggcccgggc gaggtcggct tcttcaccgc cgccatcaag
       61 caagtcgccg acacgcgcgt cggcgatacc gtcaccgacg agaaaaaccc cacggccaca
      121 ccgctgccgg gcttcaagcc gacgcagccg gtggtgttct gcgggctgtt tccggtcgat
      181 gccgcccagt tcgaggattt gcgcgaggcc atggcgcgcc tgcgcctcaa cgatgccagc
      241 ttttcctacg aaacggagag ctcggccgcg ctcgggttcg gcttccgctg cggctttctc
      301 ggtttgctgc acctcgaaat catcaccgag cggctggagc gcgaattcaa cctcgagctc
      361 atcaccaccg ccccgagcgt gatctatcac gtctactgca gcgatggcac gcggctggag
      421 atgcacaacc ccgccgacat gcccgatccc gtgcagatcg atcggatcga agagccctgg
      481 atcgaggcca ctattctggt gcccgacgaa cacctgggct cggtcttgaa gctgtgccaa
      541 gaccgccgcg ggcggcagaa gaacctcact tacgcgggca cacgtgcgct attggtctac
      601 gagttgccgc tgaacgaggt cgtgttcgac ttttacgatc gcctcaagtc catcagccgc
      661 ggctatgcct ctttcgatta tcacctgagc ggctatgaag tgggcgatct cgtcaagatg
      721 tcgatcctgg tcaacgccga accggttgat gccctgtcga tcatcgtgca tcgcgcgcgc
      781 gccgaaagcc gcggccgggc catgtgcgag aagctgaaag agctgatccc gccgcacctg
      841 ttccaaatcc cgatccaggc ggcgattggt ggccgcatta ttgcgcgcga gaccattcgg
      901 gcgctgcgca aggacgtgct cgccaagtgc tatggcggcg atatcacccg caagcgcaag
      961 ctgctcgaaa aacagaagaa aggcaaaaag cgcatgcgcc aattcggtga ggtggagatc
     1021 ccgcaggaag ccttcatcgc cgcgctcaag atggaggaga actgaggctc gcggtcaccg
     1081 cctcgaggtg accttgcgcg cgcctcgcgc cgagcgcggc ttcgctcggc cgctgtggcc
     1141 ggttcagcat cgccaccaaa gcgtagctga tgatcatcag cagaaaccag gcgccgagct
     1201 tgctcgaggg caccagcgcc cagccgtgcc gttgatgcgg gtacatccag gctgccgtga
     1261 aggtgccgat gttctcggca acccagatga agagcgcgac cagaagaaag ccgagcagca
     1321 gcggcatgcg ccgccacacg cgccagacct tgaaatagat ccaacagcga ccgaacaaga
     1381 cggcggtgac cgcaaacagg ccaaggcgca gatcggtcac gtagtgatgg gcaaagaagt
     1441 tcgcatagat gcccacaccc aatagaacca cccagccgag cggggggtgg tgggtaaagc
     1501 ggaaatcgaa caggcgccag cagcgcgcga tataggagcc gatggcggca tacatgaagc
     1561 cggtgaagag cggcacgccg cccaggcgca gaagcgaggc ctccggatag atccaggagc
     1621 cgaccgaggt cttaaagact tccatggccg tgcccacgac gtggaagaca aagatcacct
     1681 tggcctcgtc ccaggtctcg agcttggcgg tgagcatgac ggcctggatg gcgacggcgg
     1741 cgagcacgag aaagtcgtag cgcgcgagcc ccgccgcggg tggatagaag aggtgcgtgc
     1801 cgatcagaca ggccagcaac agcccaccaa acaacgaggc ccagccctgt ttgatgccga
     1861 agcgcacgaa ttcgtagagg atgagggtgt ggcgccggcg ttcggcccaa tcaccgatac
     1921 gtgcctcctg cgcgattaaa cgcgcgagtg gcgcccaatg gcgcgccccg ctcagggtcg
     1981 gtagctcggg cgtggcgggg ctttcgagcc ggccttgacc ggccagcggc ggcgcaacac
     2041 cgccgcggcg gttggacggg tccggcgtgg gcgcaacggc aagcccccgg tggagcgtac
     2101 gcctggcggt tcgctcgtgt tggcggcgtt gccccatccc gcaccttcgc cctctcggtc
     2161 ctcgtcggcc gctttggcca cgcctcgggg cccgtgcact ccccttgggc gttctggccc
     2221 tcagtcctaa gggcctcgtg cccttcgcgg gcgggttgtg tcgcccgccg ctgccggggt
     2281 ggccgcaatc ggctactggc cttgccgccc tgagttcact agattcacgg ggagattgag
     2341 gcgagccgcc tggcaacggc gcctggcgcg ccgcagcagg cccgtttccc gcgccatggc
     2401 cgggcgcggc tcggatcgaa gaggcagact ttggggtcag ataggcaagg cgtcatgggc
     2461 gcaatccccg atttcacaca agttggcttt gccgcgccgc caccggtgcg ccggctcggc
     2521 ggcggcgagc cctggctcac ccccgagggc atcgccgtca aagacgccta tgcggccgcc
     2581 gacagcgcgg gcctcgattt tctcgacggt tatccgggca tcgcgcccta cctgcgcggg
     2641 ccttatccca ccatgtatgt cacccaaccc tggaccatcc gccagtatgc gggattttcc
     2701 acggccgagg attccaacgc cttctaccgg cgcaatattg ccgccggtca gaagggcctt
     2761 tcggtggcct tcgatctgcc gacccaccgc ggttacgaca gcgaccatcc tcgtgtcaaa
     2821 ggcgatgtcg gcatggccgg tgtcgccatc gattccattt acgacatgcg cacgctgttc
     2881 gacggcatat cgctggccga catgaccgtg tccatgacca tgaacggcgc cgtactgccg
     2941 gtattggcgc tgttcgtcgt ggcagcggag gagcagggcg tgccgccgca gcgcctggcc
     3001 ggcaccattc agaacgacat cctcaaagaa ttcatggtgc gtaacaccta tatttatccg
     3061 cccgagcctt cgatgcggat catctcggac atattcgcct acaccagcgc gaacatgccg
     3121 aagttcaatt cgatttccat ctccggctat cacatgcagg aagcgggggc gacggcggac
     3181 ctggagctcg cctatacgct ggctgatggc gttgagtatg tgcgcgccgg cctcgccgcc
     3241 gggctcggca tcgacgcctt tgcgccgcgg ctctccttct tctgggcgat cggcatgaac
     3301 tatttcatgg agatcgccaa aatgcgcgcc gcgcgcctcc tatgggcgaa gctcatcaaa
     3361 gcggcggcga agccgaaaga ccccaaatcc ctggcactgc gtacgcactg ccaaaccagc
     3421 ggctggagcc tgacggcgca ggacgtcttc aacaacgtga cgcgcaccgc gatcgaggcg
     3481 ctggccgcga cccacggggg cacccagtcg ttgcacacga atgcgctcga cgaagccatc
     3541 gcgctcccaa ccgacttttc ggcccgcatc gcgcgcaaca cgcagctgct cattcagcaa
     3601 gagaccggga cctgccgggt gatcgaccct tggggcggca gccattacgt ggagcatctc
     3661 acctacgcgt tgctgcgcag ggcgcaagag cacatcgccg aggtagaagg gctcggcggc
     3721 atggccaagg cgattgccgc cggcattccc aagatgcgca tcgaggaagc cgctgctcgg
     3781 acccaggcac gcatcgatgc cggtcaacag accatcgtcg gggtcaacaa gtaccgggtt
     3841 ggcgatgccg ccgcgatcga ggttctgaag gtcgacaaca agggcgtgcg tgaccggcag
     3901 atcgccaagc tcgagcgcct caaggccgag cgcgatcctg ccgcggtggc ggcaacactc
     3961 gccgcgctga ccaccacggc gcaaagcgga gaaggcaatc tgttggcgtc cgccattgct
     4021 gccgcccgcg ccaaggcgac ggtcggggaa atctcggagg cgatggagaa ggtctggggt
     4081 cggcatcgcg ccgaggcacg ggctgtctcg ggggtctaca cggcagaggc aggcgccatg
     4141 agcaaagctg tcgagcgcgt gcgcagcatg tgccaagact tcgagaaaaa cgacgggcga
     4201 aggccccgca ttctggtggc gaaaatgggc caggacggcc acgatcgtgg gcaaaaggtg
     4261 atcgcctcgg ccttcgccga tctgggtttc gatgtcgata ttgggccgct gtttgcgacc
     4321 ccggaggagg ccgcccgcca agccgtggag aacgatgtgc atatcattgg cgtctcctcg
     4381 ctggcggccg ggcacctgac cctggtgccg gagttgacgg cggcgctcac caaggagggg
     4441 cgcggcgaca tcatgatcgt ggtcggcggc gtcattccgc cggccgacta cgaggcgctg
     4501 tttcaggcgg gcgcccaggc cgtgttcggg cccggtacca atatcccgag cgccgccgcg
     4561 gatcttttgg acaagctcaa tgcggcgcgg ggctacgggc cgcgagccgc cgccgagtag
     4621 aaaacggcgg tcgcggccac ctttttggca gggccgcgca acctcgcccg cctgcgccgc
     4681 ccgcaggttg ccaccgtcat aagcgccgca gccaggccgt gaccgtcttc accacctctt
     4741 ggtccggacc tgcaaagcca tggtgtgagc acgcctcaca cggctccccg ctgccggccg
     4801 agccgccgtc aagcggacga ggtcgaccct attggcgccg cgcaggagct ttttcggccg
     4861 gttcgccccg gccgcggggg tataggggca gccgtcgttg gcgtgaaaaa cgagcaggat
     4921 cggctggcca atgcggtgca gaccaccgac gttgcgctca acggagggct ggcgatcgtt
     4981 gatgtggatc agcatgccgg aggtgatcac aagtgcgtcg gggcgttcgg gccgttgcgt
     5041 cagctcggcg gcggccttgg cgaccgagcg cggcgcggct ggtgccgatc aggctgaccg
     5101 gcgcggcgat gcggcgcata taggcaacgt aggcaccaat atcgcgggca taggcacccg
     5161 accagcgata gcccgcgacg ccgccattgc cctgcttcaa gtccgcggcg aggtccggca
     5221 aagccagccc tgctgtagtc ggcgcggggg cgcacgagct ggttgccgcc gccccaaccg
     5281 atcgttccat ctgcaccgag ggccaaaatg ccatgaccgc cggcgagcag gatgacgcta
     5341 cccaccggcc gcgccggcgt gtgaagcaag gcgcgtaccc gctgattgcg gctgacgatt
     5401 tccaggaccg tctcgctttc ggccagcgcg acgtcgaggc ctggcaaggt aaaaaaacaa
     5461 ggctgcggcg gccaacagcg ttcgcatgct catcatcacc agcctccgca tccgcatcgc
     5521 cagcgtcccg tgcgtgcgca aactgcgcgg gccccattct cgagcttgcg accgggccca
     5581 gacttctcct acgttgatct tagccatccc gtaggacgcg aggtagaggc ggcacaatgg
     5641 cagatcgaag cgcgatcttg gctctcgacc aaggcacgac gtcgagccga gccatcctct
     5701 tcgggtcgga cttgagcgtg cttggcgcgg cgcaacgcga gtttgcccag cactttccgg
     5761 ctccgggctg ggtggaacac gagcccgagg atatctggcg caccacggtg gagaccgcgc
     5821 ggcaggcgct cgccggcccc gatcgttccc ccgccagcat cgccgggctc ggtatcacca
     5881 atcagcgcga gacttgcctg ctgtgggacc ggcgcacgag ccagccgatc catcgtgcca
     5941 tcgtttggca ggaccggcgc acggccgagg tgtgcggcgc tctcaagcgg caagggcacg
     6001 agccggagat taccgcccgc acgggcctcc tgctcgatcc ctacttttcc gcgagcaaga
     6061 tcgcctggct tctcgagcat gtggagggtg cgcgcgccct ggcggcggcc ggccatcttg
     6121 cctttggcac catcgacacc tttctgatct ggcgcctgac gggcggccgc gtgcatgcga
     6181 ccgatgccac caacgcctcg cgaacgctgc tttatgacat cggcaagggc gccttcgacg
     6241 acgatctgct caggctcttc aaggtgccgc gctcgattct gcccgacgtg ttcgattgca
     6301 atgccgaatt cgggttgacc gagccatcga tcctgggtgc cgcggtgccg atttgtggtg
     6361 tcgccgggga tcagcaagcg gccctgatcg gacaggcttg cttcacgccc ggcatggtca
     6421 aggcaaccta cgggaccggc tgttttgccg tgctcaacac gggcagcgaa caggtggcct
     6481 cgcacaacag gctcctcacg acggtggcct accagctcgg cggccagcgc acctatgcgc
     6541 tcgaaggctc gatttttatc gccggcgccg ccgtgcaatg gctgcgcgac ggcttgcgtt
     6601 tgatcgcctc ggcggcggag agcggtcggc tggccggcgc cgcggatccg gcgcaggatg
     6661 tctaccttgt gccggccttt gtggggctgg gcgcgcccta ctggcagccc cacgcacgcg
     6721 gcgcgctgtt cgggctgacg cgggccacag ggcccaacga ggttgcccgc gccgcgctcg
     6781 aggccgtttg ctatcagacg cgcgacctat tcgaggccat gcagcaggat tggcgggccg
     6841 gcgccggggc ggtgttgcgg gttgacggcg gcatggtggc gagcgactgg accatgcagt
     6901 tcttggccga cgttctcgat cggccggtgg aacggccgac gatcagcgaa acgacggcgc
     6961 tgggggcggc ctggctcgcc gggcacaagg ccggcgtttg gcccgacgca gagggcttcg
     7021 ccaggcagtg gaaactcgag cgcgccttcg cgccaaagct gtcggcaagc gaacgggagc
     7081 gcaaatatac cggttggcgg cgttcggtcg ccgccgtgct cgaggcggca aaggacgtgc
     7141 gaggagaagc atgacgcaga tcgacaacct cgaggcgttg cggcaggtct acgcgcagcc
     7201 gcgcgatctt gccctgcgca aggagctcga tcatatcgac cggcatagcc gcgagttcat
     7261 cggccgcgcc ccctttgtgg tgattgcgac ggcggacaaa gacggttggc cggatgcttc
     7321 cccgcgtggc gacgcgccgg gcttcgtcac cgtgctcgac gagaagcacc tgcacttacc
     7381 cgaccgtccc ggcaacaatc ggctcgacag tttccagaac ctgctgggca acgccaagat
     7441 tgctttgctt tttctggtgc cccggcgctt ggaaacgctg cgcgtcaatg gcaccgctcg
     7501 cctgttgacg gatccagagc tgtgcgcgcg gcatgccgta gcgggcaaac ccgcgcgcgc
     7561 gatcctggag gtcaccgcct gtagcgttta tttccaatgc ggcaaggcgc tgctccgatc
     7621 ggcgctctgg cagctggagt gctggccgcc gctcgatggc ctcgccagtt tcgccgaggc
     7681 gcttgccgac cagattaaag ggctcgacaa ggttgcctcg caaagccggc tggagaaggc
     7741 ctatcgcgac acgctgtact gagcctcgtc gcgggcgaag gcgacgatag cctcgagcac
     7801 gtccctggca cggtcgcgat gcggggcgtg cccacagttt gttagcacca aacgcgaaac
     7861 ggggccttgc gcccgtgcct cgatgctgtc gagctgagcc agagtgccgt attcgtcgtc
     7921 ggcgccttgg atgagcagga tcggcgtgct gatggcacgg gtgaggtctt cgatgttcca
     7981 gctttggaat tccggtgcca gccagacgtc ggcccagccc aggaaggcgt cgtcgacgcg
     8041 agcgtggtat ttggcgagcc ggttcctgag accgctcgcg tcgtcggcgg tcgagcaata
     8101 ggtggcgcgg atattggcga tgctcccaac acacacgggc tcgacgaagg agtgcggtgc
     8161 catcagcacc agtccggcca gcgggcgtcg gccacaggca gcatgaatga gggcgataga
     8221 ggccccgtcc gaatggccga tcagcaatgg acgttcgatg ccggttgcat cgagcaaggc
     8281 cggcaagacc gtcaatgcct cctcgtgcat gaattgcggg gcgcgcttgc cgttaaggcc
     8341 gtcggattgt ccgtagccga agcgcgagta gatgagcgcg cgcgcaccaa ggcgtgccgc
     8401 cacggaatcg ggaaaatcgc gccacagttc ggcggagccc aaaccctcgt gcaggaacac
     8461 caacggcggg cgtccaggcg cgccggggat cgagcgatag gcaatccgcc gctgtccaat
     8521 gagggcaaag ggcatatggt ttcctggcgc cgcgctgtcc gttgcgagca gctcgtcagc
     8581 ttagcagaat ttcgggtttg tgc
//
LOCUS       DASTMT010000388         1928 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_5687731, whole genome shotgun sequence.
ACCESSION   DASTMT010000388 DASTMT010000000
VERSION     DASTMT010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 1928)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1928)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1928
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5687731"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..582
                     /gene="nth"
                     /locus_tag="VFR00_09105"
     CDS             <1..582
                     /gene="nth"
                     /locus_tag="VFR00_09105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013912715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEU0159461.1"
                     /translation="LVAVVLSAQATDAGVNKATRDLFKMVATPQAMLALGLTQLEQRI
                     KTIGLYRNKAKNVIALSRQLIAEHGGAVPRERAALEALPGVGRKTANVVLNIAFGEST
                     LAVDTHVFRVAHRLKLAKGKTPLEVELGLLRVIPEEYLRHAHHWLILHGRYICQARKP
                     KCDACIVCDLCSSPDKWFDCGRPANTAKGKTQT"
     gene            579..1763
                     /locus_tag="VFR00_09110"
     CDS             579..1763
                     /locus_tag="VFR00_09110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007598984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEU0159462.1"
                     /translation="MSVNQGREFLSIPGPTNVPDEVLAAMHRPAIDIYSGEMIGITDS
                     CLADLARIFRTKGRTYIYAANGHGGWEAALTNVLSRGDLVLALESGRFATGWGEMAAM
                     LGVEVEVLKGDWRRAVDPAALEERLRKDKAHRIKAILVVQIDTASGVVNDIPAMRKAL
                     DAATHPALLMVDAVASLGCMPFDMDGWGVDVTMAGSQKGLMTPPGLAFVAANDRARAA
                     HQTAGLRTLYWDWTFREGEIHYQKYCGTPPEHLLFALRKALDMLLAEGLEAAVRRHAL
                     LAQATRVAVERWAEGQVLAFNITNPAERSNSITNVLMQGRDPRPLLAYCRDKCGVILG
                     VGLGALEGKAFRIAHMGYSNAPMLLGTLGAVEMGLKALGIPHGAGGVGGAIDYLGDAV
                     PA"
     gene            complement(1821..>1928)
                     /locus_tag="VFR00_09115"
     CDS             complement(1821..>1928)
                     /locus_tag="VFR00_09115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020012068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEU0159463.1"
                     /translation="PETPFGGVKDSGFGHEGGSEGLQVYLQSKFVSHLG"
BASE COUNT          341 a          627 c          630 g          330 t
ORIGIN      
        1 ctggtggcgg tggtactttc cgcacaggcg accgacgctg gggtcaacaa ggcaacgcgc
       61 gatttgttca agatggtcgc tacgccacag gccatgttgg cgctcggcct cacccaactc
      121 gagcagcgca tcaagaccat cggcctttac cgtaacaagg ccaaaaacgt catcgcgttg
      181 tcacgccagc tgattgccga acacggcggc gccgtgccgc gcgagcgtgc cgcgctcgag
      241 gccttgcccg gcgtcggccg caagacggcc aacgtcgtgc tcaacatcgc cttcggcgaa
      301 agcacgctgg ccgtcgatac gcacgtcttc cgggtggcgc atcggctcaa gctggcgaag
      361 ggcaagaccc cactcgaggt cgagctcggc ctcttgcgtg tgatccccga ggaatacctg
      421 cggcacgcgc atcactggct cattctgcac ggccgctata tttgtcaggc gcgcaagccc
      481 aaatgcgatg cctgcattgt ttgcgacctg tgctcttcgc cagataagtg gttcgactgc
      541 ggccggccgg ccaacacagc taaagggaaa actcagacat gagcgtcaat caaggccgag
      601 agttcctgag catccctggc ccgaccaacg tccccgacga ggtgctggct gccatgcacc
      661 gcccggccat cgacatttat tccggcgaga tgatcggcat caccgatagc tgccttgccg
      721 atcttgcccg cattttccgc accaagggcc gcacctacat ctacgccgcc aatggccacg
      781 gtggttggga ggcagcgctc accaacgtgc tgagccgggg cgatctcgta ctggcattgg
      841 agagcgggcg tttcgccacg ggctggggtg agatggccgc catgctcggt gtcgaagtgg
      901 aggtgctcaa gggcgattgg cgccgagcgg tcgatcctgc ggcgctcgag gagaggctgc
      961 gcaaggacaa ggcgcatcgc atcaaggcaa ttctggtcgt gcaaatcgac acggcctcgg
     1021 gtgtggtcaa cgacatcccc gccatgcgca aggccttgga tgcggccaca cacccagcct
     1081 tgctcatggt ggacgccgtc gcctcgctcg gctgcatgcc cttcgatatg gacggctggg
     1141 gcgtggacgt gacgatggcc ggctcgcaaa aggggctaat gacgccgccg ggcctcgcct
     1201 tcgtggccgc caacgatagg gcgcgcgcgg cacaccagac ggcaggccta cgcaccctct
     1261 actgggattg gacctttcgc gaaggagaaa tccactacca aaagtactgc ggcacgccac
     1321 cggagcattt gctgttcgcg ctgcgcaagg cgctcgacat gctgctggcc gaaggcttgg
     1381 aagccgccgt ccgacggcat gcgttgctgg cgcaggcaac gcgcgtggcg gtcgagcgct
     1441 gggccgaggg tcaggtcctg gccttcaaca tcaccaatcc cgccgagcgc tcgaattcga
     1501 tcactaacgt cctgatgcag ggccgcgatc cgcgaccgct gctcgcctat tgccgggaca
     1561 aatgcggcgt cattctcggc gtcgggctcg gcgccttgga aggcaaggcg tttcgcatcg
     1621 cccacatggg ctacagcaac gcgccgatgc tgctgggcac gctgggggcc gtcgagatgg
     1681 gtctgaaagc gctgggcatt ccgcacggcg ccggcggcgt cgggggcgcc atcgactatt
     1741 tgggtgatgc cgtccccgcc tgaggggcgc ctagcgaggc gggcggccgc ccgaggcagc
     1801 cgcctccggg gttggcgcga tcagccgagg tggctcacga acttggactg caggtagacc
     1861 tgcaggcctt cgctcccgcc ttcgtggccg aagcctgaat ccttgacgcc gccaaacggt
     1921 gtctcggg
//
LOCUS       DASTMT010000389         4293 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_2508420, whole genome shotgun sequence.
ACCESSION   DASTMT010000389 DASTMT010000000
VERSION     DASTMT010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4293
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2508420"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..560)
                     /locus_tag="VFR00_09120"
     CDS             complement(<1..560)
                     /locus_tag="VFR00_09120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0159464.1"
                     /translation="MLRIGAVTLAVLAALVGTVDASAAQAATPGDWSRYQNERFGFRL
                     EYPANLFHVERTTEAGDGRVFAARNGEARLLVGALRNEDRHSAKSYQDFLARTSYADY
                     TINYRPLGDDWLVLSGEANGKIFYEKAILSCGGELISSFAMLYPADKRDVFEPIVARV
                     ANSFVPGTKPCRAESASPRHRPHGEA"
     gene            complement(654..1442)
                     /gene="xth"
                     /locus_tag="VFR00_09125"
     CDS             complement(654..1442)
                     /gene="xth"
                     /locus_tag="VFR00_09125"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014285794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEU0159465.1"
                     /translation="MRIATWNINGAKARLEGALTWLKETQPDVACFQEIKSTNEAFPA
                     AMFEELGYHVAVHGQKGFNGVAVLSKPRLEEVVVRGLPGDAADAHARYIEVVVPNGSA
                     TGRVGNLYLPNGNPIGTDRFAYKLAWMRRFEARTRELLTLEEPFLLVGDYNVIPEPAD
                     AKHPDAWREDALFQPETRAAFRALLNLGLTDAVRACQPAAGIYTFWDYQAGAWQKDNG
                     IRIDHVLLSPQAADRLMAAGVDKFTRAWEKPSDHVPVWVELADG"
     gene            complement(1518..1922)
                     /locus_tag="VFR00_09130"
     CDS             complement(1518..1922)
                     /locus_tag="VFR00_09130"
                     /inference="COORDINATES: protein motif:HMM:NF017837.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF930 domain-containing protein"
                     /protein_id="HEU0159466.1"
                     /translation="MRIRFFLRRLAGGLGLAGALLAGTAAAQTSLDKRMQALEPHTRM
                     IQLCNLSGLETFAKDKSVPKVDRVRIDALAAPTVESNVARGAGGAVRTKGQWYKFSYR
                     CQFTPDRMRATHFSYVLEREIPRAQWEKFNLF"
     gene            complement(2065..2397)
                     /gene="erpA"
                     /locus_tag="VFR00_09135"
     CDS             complement(2065..2397)
                     /gene="erpA"
                     /locus_tag="VFR00_09135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009799806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HEU0159467.1"
                     /translation="MEDPKVTVSERAAQRIAQIVAGEPDKPLLRVSVEGGGCSGFQYK
                     FDLVGASEVDDLIIERAGARVLIDQVSLAYLAGSEIDFVDDLIGSSFKVINPNATASC
                     GCGTSFSI"
     gene            2495..3721
                     /locus_tag="VFR00_09140"
     CDS             2495..3721
                     /locus_tag="VFR00_09140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HEU0159468.1"
                     /translation="MAAAMIDAPAAAAAALEPRARPLAPYATEPALSAGRLYREPACP
                     MRTPFQRDRDRIVHATSFRRLTYKTQVFIFHEGDHYRTRLTHSLEVAQIARAIARLLW
                     LDEDLAEALALAHDLGHPPFGHAGERALAAAMADFGGFDHNAQSFKVVTRLERKYASF
                     DGLNLSFETLEGLAKHNGPLAAPGVEASPLYAAIAASGLADQLHLSLFAPAEAQVAAI
                     ADDIAYDSHDLDDGLRAGLLRIEELAAVPLVGRFLADVPPHTLDTQRLIYEINRRMIT
                     AMIDDVVRVSRARLASLHHPSLEAVRRAPGPLIAQSERLAGEIEGLKAHLYAHVYRHP
                     RVMRIMADAEAIVADLFACYLAQPQEMSGALQLAAQAANGRDRAGIVADFVAGMTDRY
                     AIKEHRRLFDATPELR"
     gene            3774..>4293
                     /gene="argS"
                     /locus_tag="VFR00_09145"
     CDS             3774..>4293
                     /gene="argS"
                     /locus_tag="VFR00_09145"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011472956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEU0159469.1"
                     /translation="MHAHALVHSRIVAALEALQHEGALPPGLDFAGTEVASPREAAHG
                     ELASNAAMVLAKPARLPPRLIAEKLAAKLAAAPDIAKAEIAGPGFVNLTFAPSFWQAV
                     VTQILKEGADYGRSDVGASQRINIEYVSANPTGPMHVGHCRGAVFGDALANLLAFVNY
                     DVTREYYINDAGA"
BASE COUNT          716 a         1454 c         1398 g          725 t
ORIGIN      
        1 ctcgcctcgc catgcgggcg atgccgagga ctggcgctct ccgcgcggca cggcttggtg
       61 cccggcacga agctgttggc gacacgggcc acgatcggct caaatacgtc gcgcttgtcg
      121 gcgggataaa gcatcgcaaa gctgctgatc agctccccgc cgcagctcag gatcgccttc
      181 tcgtagaaga tcttgccatt ggcctcgccc gacaatacga gccaatcgtc gcccagcggt
      241 cggtagttga tggtgtagtc cgcataagac gtgcgcgcta gaaagtcctg gtagcttttg
      301 gcgctgtgcc ggtcctcgtt gcgcagcgcc ccgaccaaca ggcgtgcctc cccgttgcgt
      361 gcggcgaaga cgcgcccatc gcctgcctcc gtcgtgcgct cgacgtggaa gagattggcg
      421 ggatattcga gccgaaagcc aaagcgctcg ttctgatagc gtgaccagtc gccaggcgtc
      481 gccgcctgtg ccgcgctcgc gtcgacggtg cccacgaggg ctgccaagac tgcgagcgta
      541 acggccccga tccgcaacat gacccaactc ctcgaccaga tgcaggatca accgtcacgg
      601 ggaggtcctg gttccggcgg cggagttgtg gcgcggctcc ggcggcgcgc agcttagccg
      661 tcggcgagct cgacccaaac ggggacgtgg tcggagggct tttcccaagc gcgcgtgaat
      721 ttatcaacgc ccgcggccat cagacggtcg gccgcctgcg gcgacagcaa gacatggtcg
      781 atgcgaatgc cattgtcctt ctgccaggcg ccggcctgat agtcccagaa ggtatagatg
      841 ccggccgcgg gctggcaggc acgcacggca tcggtcagcc cgagattgag gagggcgcga
      901 aatgcggcgc gcgtttcggg ttggaagagc gcgtcctcgc gccaggcatc gggatgcttg
      961 gcgtcggcgg gttcgggaat gacgttgtag tcgccgacca acaagaatgg ctcctccagc
     1021 gtcaagagct cgcgcgtgcg ggcctcgaag cgccgcatcc aggcgagctt gtaggcgaag
     1081 cgatcggtgc cgatcgggtt gccgttcggc agatataggt tgccgacccg accggtagcg
     1141 ctgccgttcg gcacgacgac ctcgatgtag cgtgcgtgcg catccgcggc atcgccgggg
     1201 aggccacgca ccacgacttc ctcgaggcgc ggcttgctca gcacggcgac gccattgaag
     1261 cctttctgcc cgtgcacggc cacgtggtag ccgagctcct cgaacatggc ggcgggaaag
     1321 gcctcgttgg tggatttgat ctcctggaaa caggcaacgt ccggttgcgt ctccttgagc
     1381 caggtgagcg ccccctcgag ccgggccttg gcgccgttaa tgttccaggt tgcgatgcgc
     1441 atggcctgtt gcacaccaag gtgccggtcg cggtcaagcc tcccgcaatg gcttggcgcc
     1501 gcgccaggcc aggacgctca gaagagattg aacttttccc actgcgcgcg ggggatctcg
     1561 cgctccaaga cgtagctgaa gtgggtcgcc cgcatgcggt cgggcgtgaa ctggcagcgg
     1621 tagctgaact tgtaccattg tcctttggtg cgcacggcgc cgccggcacc ccgggcgaca
     1681 ttgctttcca cggtgggcgc ggccaaggcg tcgatgcgca cgcggtcgac tttgggcacg
     1741 ctcttgtcct tggcaaaggt ctcaaggccc gacaaattgc acagctggat catgcgcgtg
     1801 tgcggctcca gggcctgcat gcgcttatcg aggctggttt gggccgccgc cgtgccggcg
     1861 agcagggcgc ccgccaagcc aaggccgccg gcgaggcgcc gcagaaagaa ccggatgcgc
     1921 aacgtgcgtc ccccttctca gtacagttgt ggcgtcgcct acggctgcgg cgccgaaccc
     1981 cccgagctgc aaagacgctg aagatgcggt gaaaaagtgg cggtgacggc ggtgcacccg
     2041 cggccgccgg caggcaagcg ccggtcagat cgagaagctc gtgccgcagc cgcaggaggc
     2101 ggtggcgttg ggattgatca ccttgaagga agagccgatc aggtcatcga caaaatcgat
     2161 ctcggagccg gccaggtagg ccaacgaaac ctgatcgatc aggacgcgtg ccccggcccg
     2221 ctcgatgatg agatcatcga cctcggaggc cccgaccaga tcgaacttat actggaatcc
     2281 ggagcacccg ccgccctcga cgctgacccg caaaagcggc ttgtccggtt ccccggcgac
     2341 gatctgcgcg atccgctgtg cggcccgctc ggatacggta actttcggat cttccatgac
     2401 tttttggccc cggacggccc atgcactatg ctggctgacg attctcagtc atgaagatag
     2461 ggtggcaggc aagcttgtca aatcgagatg gagcatggct gcggcgatga ttgatgcgcc
     2521 ggcggcggcc gcggcggcgc tagagccccg ggcgcgcccg ctcgcccctt acgcgaccga
     2581 gcccgcgctc agcgctggcc ggctttatcg cgaacccgcc tgccccatgc gcacgccctt
     2641 ccagcgcgac cgcgatcgga tcgtgcatgc cacctcgttt cgccgcctga cctacaagac
     2701 gcaggtcttt atttttcacg agggtgacca ttatcgcacg cggctgaccc acagcctgga
     2761 ggtggcgcag atcgcccgcg ccatcgcgcg cttgctctgg ctcgatgaag acctcgccga
     2821 ggcgctggcg ctggcacacg acctcgggca tccccccttc ggccacgccg gcgagcgcgc
     2881 gcttgccgcg gccatggcag acttcggcgg tttcgatcac aacgcccaaa gcttcaaggt
     2941 cgtgacccgg ctcgaacgca agtatgcgag cttcgacggc ctcaacttga gcttcgagac
     3001 gctcgagggc ttggccaagc acaatggccc cctggccgcg ccaggcgtcg aggcgagccc
     3061 cctttatgcc gccatcgccg cgagcggcct cgctgaccaa ttgcacctat ccctgtttgc
     3121 cccggccgag gcgcaggtcg cagccattgc cgacgatatc gcctacgaca gccacgatct
     3181 cgacgatggc ctgcgcgccg gcctgctgcg gatcgaggag ttggcggcgg tgccactggt
     3241 cggccgcttt ctggccgacg tgccgccgca cacgctcgac acccagcgcc tgatctacga
     3301 aatcaaccgg cgcatgatca cggccatgat cgacgatgtc gtgcgggtct cccgcgcgcg
     3361 cttggcaagc ttgcaccacc caagcctcga ggcggttcgc cgggcgccgg gccccctcat
     3421 cgcccagtcc gagcgcctcg ccggcgagat cgagggcctc aaggcccacc tttatgccca
     3481 cgtctaccgc catccccgcg tcatgcggat catggccgac gcggaggcca tcgtcgcgga
     3541 tctgttcgcg tgctatctcg cgcaaccgca ggaaatgtcg ggcgccttgc agctcgccgc
     3601 gcaagccgcc aacgggcgtg accgggccgg catcgtggcc gatttcgttg ccggcatgac
     3661 cgatcggtac gccatcaagg aacaccgccg cttgtttgac gctaccccgg aattgcgtta
     3721 gtcggccccc gcccgccagc gcggcccggc cccggatcga ttggatgtcg ccgatgcatg
     3781 cccatgccct tgtgcacagt cggatcgtgg ccgcgctgga ggcgctgcaa cacgagggcg
     3841 ccttgccgcc gggcctcgat tttgccggca ccgaggtggc ctctccgcgc gaggcagcgc
     3901 acggcgagct tgcctccaat gccgccatgg tgctggccaa accagcgcgc ctgcccccgc
     3961 gcctgattgc cgaaaagctc gcagccaagc tggccgcggc gccggatatc gccaaggccg
     4021 agatcgccgg ccccggcttc gtcaacctga cattcgcgcc gtcgttttgg caggcggtcg
     4081 tgacgcagat cctgaaagag ggggccgact atgggcgcag cgacgtcggg gcgtctcagc
     4141 gcattaacat cgagtacgta tcggccaatc cgaccggccc catgcacgtg ggacactgcc
     4201 gcggcgccgt gttcggcgat gccctcgcca acctgctggc cttcgtcaac tacgatgtta
     4261 cgcgcgaata ctacatcaat gacgcgggcg ccc
//
LOCUS       DASTMT010000390         5116 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_4152074, whole genome shotgun sequence.
ACCESSION   DASTMT010000390 DASTMT010000000
VERSION     DASTMT010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 5116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5116
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4152074"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..292
                     /locus_tag="VFR00_09150"
     CDS             <1..292
                     /locus_tag="VFR00_09150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008837130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HEU0159470.1"
                     /translation="LTKRLEALIAPPGFSGGQPGGPSRWSTERSSAWQPLRPSLVAEV
                     RYDHVSGGRFRHGTKFLRWRPDKSPRQCTLEQLSQARPARSAGGRRRLTVLP"
     gene            374..1153
                     /locus_tag="VFR00_09155"
     CDS             374..1153
                     /locus_tag="VFR00_09155"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEU0159471.1"
                     /translation="MALADTPRSSLAASAPMLRLGRLAGLVYGLAGVAIMAVFWPLFG
                     VFLSNAPRLGSPWLERSADVGLGVANPYFAGAIDLALIAAFGLQHSLMARPALKRRWR
                     GLVPPALERATYVHAANIVLLALVVFWQPIPIEVWRVEGSLLRDACWALFALGWVILL
                     AGAVSFGIAELLGVRQVLDWYWEREPRPLPLKTQGLYRWLRHPMYVGVLLAVWATPYM
                     TLGHGLLAAGLTVYVLIAKRYEERDLDRTYGRLYQTWTRAR"
     gene            complement(1234..1431)
                     /locus_tag="VFR00_09160"
     CDS             complement(1234..1431)
                     /locus_tag="VFR00_09160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009735998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6496 domain-containing protein"
                     /protein_id="HEU0159472.1"
                     /translation="MARRYSKKASKTVKTAMSRRKRGTLKSGRSGKTVKSRKQAIAIG
                     LSEARRKGQKVPARKKAGKKK"
     gene            1640..1804
                     /locus_tag="VFR00_09165"
     CDS             1640..1804
                     /locus_tag="VFR00_09165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008834744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3309 family protein"
                     /protein_id="HEU0159473.1"
                     /translation="MTLSTVLLIILILALLGALPSWPYSRGWGYGPSGLVGVLLVVVL
                     LMALMGRMPA"
     gene            2065..3612
                     /locus_tag="VFR00_09170"
     CDS             2065..3612
                     /locus_tag="VFR00_09170"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like serine protease"
                     /protein_id="HEU0159474.1"
                     /translation="MAVLTCGLLLPLQASWAQARQCLSARSKIVGGETARLADWPGIA
                     TVRLHSDNGQVSRYFCGAAAISERWILTAAHCLPSYVGRLTGSLRNSRGEEHVGRLEV
                     VLGTGDLRGAKPEQVYPVEAVVMHEHYRAAVEAAFRRAEGEQADALEAIAPSQGDDIA
                     LLKLARPWGGQLSRISLSGTTDPEPSGSAQVRVAGFGYTSTQRLNRFNRSDGRGEFYA
                     GSAVLLETAVETVAQTTCKGRYANAAIGAGQLCAGLEEGGKDSCQGDSGGPLIMTDAQ
                     GCPWQVGLVSWGEGCAERKAYGVYTRISHYADWIQKHTGPLAGAAPMLQASGAGALSP
                     VLLEEGLKQLETLLGPSKGRLKLGIRGGNRVRLGQQVIFEASSDIAGRLIILDINASR
                     EVTLIYPNRFVAAADPGRIRAAQQVAVPGADYPGFTAFQAVEPIGKGELLAVVAPEDF
                     DVERFAANKTVVSKGFQPVNDPPSFLMRIIRQIEVALAARARAGGSERDELQRWGYAL
                     TRYEIVK"
     gene            complement(3630..4580)
                     /locus_tag="VFR00_09175"
     CDS             complement(3630..4580)
                     /locus_tag="VFR00_09175"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:NF012862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HEU0159475.1"
                     /translation="MRKLVFLIMGAALALSALQSASQAAPEKRVALVIGNAAYAGISP
                     LQKPINDAQKMAEVLRGLNYEVILGINASKRELDVLAQRFRAALKGADVGFFFYSGHG
                     FQTNRIEQQHPINHIVPVDFNVQDVNTALGSLALDVIIEAIRSQARVGFIFMDACRND
                     PQLTAASERLASNTRSVTIARGFAPVAAIEKTSTAKGAPAKGPTGLLIAYATDPGNVA
                     LEGDTGTLSPFTGALSKHLVTPGLSAAEIMGRVSAEVATQTGGQQTPWNVSSLTAGTY
                     LFTPRPAAPAGSGTTAKGPGARPAGSSLPPNLGVGVGSGL"
     gene            complement(4754..>5116)
                     /locus_tag="VFR00_09180"
     CDS             complement(4754..>5116)
                     /locus_tag="VFR00_09180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEU0159476.1"
                     /translation="NAIMMIDFAIEAEREQGLSSRDAIHEACLLRFRPIMMTTMAALF
                     GTLPIAVGWGAGAELRQPLGLAVVGGLAVSQLLTLYITPAIYLMMEKLGTRLGAGRRE
                     HIPASPGTAELERPLAAE"
BASE COUNT          774 a         1646 c         1768 g          928 t
ORIGIN      
        1 cctgaccaag cggctcgagg cgttgattgc gccgccgggc ttcagcggag gccaacctgg
       61 tgggccaagc cgctggagca ccgagcgttc gagcgcctgg cagccactgc ggccgagcct
      121 ggtagcggaa gtgcgctacg accacgtcag cggcggccgc ttccggcatg gcaccaagtt
      181 tctgcgctgg cgtccggaca agtcgccccg tcagtgtacg ctcgaacagc tgtcgcaagc
      241 gcgcccagcg cggtccgccg gcggccggcg gcggctcacc gtcctgccgt gactgtactt
      301 tcgccacgtc agccaggaac tcttgggctt gggcggggtt gccgatccgg caggtgatga
      361 gggagtgcgt cgcgtggccc ttgctgacac ccccagatcc tcgctcgcgg cgagcgcacc
      421 gatgctgcgc ctcgggcgcc ttgccggcct cgtctatggt ctcgctggcg tcgccatcat
      481 ggcggtgttc tggccattgt tcggggtgtt tctcagcaat gcgccgcggc tgggctcacc
      541 ttggctcgag cgctcggccg acgtggggct cggggtagcc aacccgtatt ttgccggcgc
      601 catcgatctg gcgctgatcg cggcgtttgg cttgcagcat tcgttgatgg cacggcccgc
      661 cctgaagcgg cgttggcgtg ggctggtacc gccagcgctc gagcgcgcga cctacgttca
      721 tgccgccaat atcgtgctgt tggcgctggt cgtcttctgg caaccgatcc ccatcgaggt
      781 gtggcgcgtc gaaggttcgc tgctacgcga tgcctgctgg gccttgttcg cgctcggttg
      841 ggtcatcctg ctggcgggag ccgtgtcgtt cggcatcgcc gagctcctcg gtgtgcggca
      901 ggtcttggac tggtactggg agcgggagcc gcggccgctg ccgctcaaga cccagggcct
      961 ctaccgctgg ctccgacatc cgatgtacgt aggtgtgctg ctggccgtgt gggccacgcc
     1021 ctacatgacg cttgggcatg gcctgttggc ggccggcttg acggtctatg tcttaatcgc
     1081 caaacgctac gaagagcgcg acctcgaccg cacctacggt cgcctctacc aaacctggac
     1141 gcgtgcgcgc tgacgcgcgc ggccccacgc tgaggtcgtc atcggttggc gcgcggcgca
     1201 ggagagcctg ccgccgagac gcaaccccag ggcttatttt ttctttcctg cctttttgcg
     1261 cgcgggcacc ttctgcccct tccgacgcgc ttctgacagg ccgatggcaa tggcttgttt
     1321 gcgacttttg acggtcttgc cgctgcggcc gcttttcaag gtcccgcgct tgcgccggct
     1381 catcgccgtc ttcacggtct tggaagcctt cttcgaataa cgtcgcgcca tctaacgctc
     1441 cttgcacagt tcacgccgag gaacgcgcag gcccattgaa atgttccccc tcgactgcgt
     1501 ggggaccgcg tgtgtccgcg ccttcgcaat tgagaggccg gaccattcgc cgttcgcccg
     1561 caaagcggct ggggaaccac gctggggtgc tgcgcattgg cgccactgat cctatcgctt
     1621 caaggcacag gagttgcgaa tgacgctctc aaccgtgttg ctcattattc tgatccttgc
     1681 cctgttgggt gcgctgccgt cctggcccta cagccgcggc tggggctatg gcccgtccgg
     1741 cctcgtaggc gtgctgctgg tggtcgtgct cctcatggct ttgatgggac gcatgccggc
     1801 ctaaggcggc cgcaaccgtc gcgaagcgac gcgccgggcc cggacgatcg gcggcacggc
     1861 tcccaaggcc ttcccgcgcg cgacctagcg gccgcgccgg ggcgggtttt tttttgttcg
     1921 cctcggccag aagctggcgc cgacttggag cggcgccgcg ctgcggctag tctggacgca
     1981 gggctcccgg tgcaaaggcg atgggggaca aggcctgggg acttgagggg ggtgggggac
     2041 atgggctcaa gggtgatgat tggcatggcc gtgctgacgt gcggcctcct gctgccgctc
     2101 caggcgtcct gggctcaggc gcgtcagtgc ttgagcgcac gcagcaaaat cgtcggtggc
     2161 gagaccgcac ggctggcgga ttggccggga attgcgaccg tgcgcttgca ctccgacaac
     2221 ggccaggtgt cgcgctattt ttgcggcgct gcagccatca gcgaacgctg gattttgacg
     2281 gccgcgcact gcttgcccag ctatgtcggc cggctgaccg gatccttgcg caattcccgc
     2341 ggcgaggagc acgtcggccg cctcgaggtg gtgctcggca cgggcgatct gcgcggcgcc
     2401 aagcctgagc aggtctaccc ggtcgaagcg gtcgtgatgc acgagcacta ccgcgccgcc
     2461 gtcgaggccg ctttccgacg ggcggaaggc gaacaggcgg acgcgctgga ggcgattgcg
     2521 cccagccagg gcgatgatat cgcccttctc aagctcgcca gaccttgggg cggtcagctc
     2581 agccgcatct ccctttcggg aacgacggac ccggagccct cgggcagcgc gcaggttcgc
     2641 gtggcggggt ttggctacac cagcacgcag cggctcaacc gtttcaaccg gtcggacggc
     2701 aggggcgaat tttacgccgg ctcggccgtg ctcctggaaa ccgccgtcga gacggtggca
     2761 caaacgacct gcaagggccg ctatgcgaac gcggccatcg gtgccggcca gctgtgcgcc
     2821 gggctggagg agggcggcaa ggacagctgt cagggcgaca gtggaggacc gttgatcatg
     2881 accgacgcgc agggttgccc ttggcaggtt ggcttggtca gctggggtga aggctgcgcg
     2941 gagcgcaaag cctacggcgt ttatacgcgc atttcgcatt atgcggattg gattcaaaag
     3001 cacacgggtc ccctcgccgg cgccgccccg atgcttcagg cctcgggtgc gggggcgttg
     3061 agcccggtgt tgctagaaga ggggctgaag cagcttgaaa ccctgcttgg ccccagcaaa
     3121 ggccggctca agctcgggat ccgcggcggc aatcgcgtac gccttggcca acaggtgatc
     3181 tttgaagcct ccagcgatat cgcaggcagg ctgatcattc tcgacatcaa tgcgagccgc
     3241 gaggtgacgc tgatctatcc gaaccgcttc gtggccgccg ccgatccggg ccgcatccgc
     3301 gccgcgcagc aggtggccgt accgggtgcc gactatcctg gatttaccgc ctttcaagcg
     3361 gtcgagccga tcggcaaggg cgagctgttg gccgtggtcg cgcccgagga cttcgacgtc
     3421 gagcgcttcg ccgccaacaa gacggtggtc agcaagggct tccagcccgt gaatgacccg
     3481 ccaagttttc tgatgcgcat catccgtcag atcgaggtcg cactcgccgc gcgggcgcgt
     3541 gccggcggca gcgagcgcga tgagctgcaa cgctggggtt acgccctgac gcgctacgaa
     3601 atcgtcaagt gaggtcgcgg cgagggtggt cagagcccgc tgccgacgcc gacaccgaga
     3661 ttgggaggca ggctcgaccc ggccgggcga gcgcccggac ccttggcggt cgtcccactt
     3721 ccggccgggg ccgctggccg gggcgtgaac agataggtgc cggcggtcag cgatgagacg
     3781 ttccagggcg tctgctggcc gcccgtttgc gtggccacct ccgccgagac gcggcccatg
     3841 atctcggcag cgctgagccc aggagttacg aggtgcttgc tgagagctcc ggtgaacggg
     3901 ctgagcgtgc ccgtgtcgcc ctcgagcgcc acattgccgg gatcggtggc gtaggcgatc
     3961 agcaggccgg tcggaccttt ggcgggggct cctttcgcgg tcgaggtttt ttcgatcgcg
     4021 gcaaccggcg caaagccgcg cgcgatcgtc acgctgcgcg tgttgctggc caagcgctcc
     4081 gacgcggccg tcagctgcgg atcgttgcgg caggcatcca taaagataaa cccgacgcgc
     4141 gcctgactgc gaattgcctc gatgatgacg tcgagggcaa gcgatcccag cgccgtattg
     4201 acgtcctgca cgttgaagtc gaccggcacg atgtgattga tcggatgctg ctgctcgatc
     4261 cggttggtct ggaagccatg gccggaataa aagaagaagc cgacatcggc tcccttgagc
     4321 gcggcgcgga acctctgtgc caggacgtcg agctcgcgct tgctggcatt gatgccgagg
     4381 atcacctcgt agttcaggcc acgtagcacc tcggccattt tctgcgcgtc gttaatgggc
     4441 ttctgcagcg ggctgatgcc ggcgtaggcg gcgttgccga tcacgagcgc gacgcgcttt
     4501 tcgggcgcgg cttggcttgc ggactgaagg gcgctgagcg cgagtgcggc acccatgatt
     4561 aggaaaacga gcttgcgcat ccaggacccc ccaccgccgt ccttctcgcc tcgaaagaag
     4621 aatagccacg aaaggctcgg caaggctagg gcaagctcgc ccgggcatcc ggcgacgccg
     4681 gagtgcgcgg gcgttttgcg cgccaaaaca accgcttggc ccgcgccagg ctggcgcgcg
     4741 aggggttgcg gcatcactcc gccgcgagcg gcctttctag ttcggccgtt cccgggctcg
     4801 ccgggatgtg ctcgcgccgt cccgcgccga gccgcgtgcc cagcttttcc atcatcaaat
     4861 agatggcggg cgtgatgtag agcgtcagca gctgcgaaac ggcgagaccg ccgaccaccg
     4921 ccaggcccaa gggctggcgc aactccgcac ccgctcccca gccgacggcg atgggcagcg
     4981 taccgaacag cgccgccatg gtggtcatca tgatgggacg gaagcgcaag agacaggcct
     5041 cgtggatggc gtcgcgggaa gacaagccct gctcgcgctc ggcctcgatg gcgaagtcga
     5101 tcatcatgat ggcgtt
//
LOCUS       DASTMT010000391         2068 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_4705201, whole genome shotgun sequence.
ACCESSION   DASTMT010000391 DASTMT010000000
VERSION     DASTMT010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2068
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4705201"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            262..651
                     /locus_tag="VFR00_09185"
     CDS             262..651
                     /locus_tag="VFR00_09185"
                     /inference="COORDINATES: protein motif:HMM:NF024498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin fold domain-containing protein"
                     /protein_id="HEU0159477.1"
                     /translation="MRLLFCVFAAVAMCALTGGGTGRAALDPAASVRGSDIELLVYEH
                     RDCVYCQLFRRDVLPRYQQALAAELPIRFVDIAAAGNEGPGLARKVDMLPTVVLMLGG
                     REIDRIVGYWGPDTFFKLLAHMRAKVG"
     gene            748..1785
                     /locus_tag="VFR00_09190"
     CDS             748..1785
                     /locus_tag="VFR00_09190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0159478.1"
                     /translation="MNRRGFLISGGAAAVAASAATDPAAAMAPREAAAGPGGAEAGGA
                     RVLTLAVAPGYDRAGFAADRLAGRISLATAGRFRIERTGAATAADLSLTDARSLATLH
                     PAFAFFAGLPLDEGLPSADQDAWLTLGGGQLLWDELARAFGFKPLVAGHTGQDGGFWS
                     SVRLNAAADLVGLPVAATGLAGRVLEVMGAKVIDVAGPDLGAALASGRVRAGEAQGPS
                     PSSAFNLEPLAQCCYRPGLYPHGVLTTLLVRSGLWEGLDAADQAIFEACTAAEHRLAL
                     AEAKMHAALATHMRRAAKWPARASLPPALAQALGQVATAAVAEVAGLDAASRRIAASY
                     RAHRELQGILA"
BASE COUNT          287 a          688 c          732 g          361 t
ORIGIN      
        1 cggcgacgcc ttcgccaagc agtttggggc cttcgcactc ggcggatccc aagcaatctc
       61 ggtcgactgt caagggccct aacccacgac tgggtggctc cggggcgcag ccgcacgggc
      121 atcgcgggcc gcttcgattg ggtcgggcgt ttacgcactc tttattgttt gcttccgggg
      181 cagcggcctt tgccgctagg ctcgggcgcg tcggtgcggc cttaagtcgg tcgaaggccg
      241 tcggttcgtg agtgtgtcta aatgcggttg ctgttttgcg tttttgccgc cgtggcaatg
      301 tgtgccctaa ccggcggcgg caccggacgc gccgccctcg atcctgcggc cagcgtgcgc
      361 ggatccgaca tcgagctctt ggtctatgag catcgcgatt gcgtctattg ccagcttttt
      421 cgccgcgatg tgctgccgcg ctaccagcag gccttggcag cggagttgcc gatccgcttc
      481 gtcgacatag ccgccgccgg caacgagggc cctggcctcg cgcgcaaggt cgacatgctg
      541 ccaaccgtcg tcctgatgtt ggggggacgc gagatcgatc ggatcgtcgg ctattggggc
      601 ccggatacat ttttcaagct cctggcgcat atgcgcgcca aggtgggcta gcaacgaggc
      661 cggcgctctt gcggcgcgtg ccatggtcgg ctttgatgcc cgctgtggtc ggcgtgcgtg
      721 ggctgtgccg gcagcggagg ggtcgccatg aacaggcggg gcttcttgat atcgggcgga
      781 gccgcagccg tggccgccag tgcggcaacg gaccccgccg cggcgatggc gccaagggag
      841 gcggcggcgg gcccaggcgg agcggaggca ggcggcgcgc gcgtgctcac cttggcggtg
      901 gcgcccggat acgatcgcgc tggttttgca gccgaccggc tcgccggccg catctccttg
      961 gcgacggccg gccgctttcg catcgaacgc acgggcgctg cgaccgcggc cgatctcagc
     1021 ctcaccgacg cccgctcgct cgccaccctg caccctgcct tcgccttttt tgccggcctg
     1081 cccttggatg agggtttgcc cagcgcggat caggatgcct ggttgacgct cggcgggggt
     1141 caactgctct gggatgaact tgctcgtgcc ttcggcttta aaccgttggt cgcgggtcac
     1201 accggtcagg acggcggctt ctggtcgagc gtgcgcctga acgcagcagc cgaccttgtc
     1261 ggcctgccag tcgccgcgac ggggctcgct gggcgcgtcc ttgaggtcat gggcgccaag
     1321 gtaatcgacg tggccgggcc ggatctcggc gcagccttgg ccagcgggcg ggtgcgcgcg
     1381 ggggaggcgc aaggaccctc gccgagctcg gcctttaacc tcgagccgct ggctcaatgc
     1441 tgttatcggc cggggctcta cccgcacggg gtgctaacca ccctcctcgt cagatccgga
     1501 ctttgggagg gactggacgc ggccgaccag gcgattttcg aggcctgcac ggcggcagag
     1561 catcgcctcg cgttggccga ggccaaaatg catgccgccc tagccacgca catgcgcagg
     1621 gcggccaagt ggccggcacg cgcatcgttg ccgcccgcgc tcgctcaggc cctgggccag
     1681 gtcgcaaccg ccgccgtcgc ggaggtcgcc ggtttggatg ccgctagccg gcggatcgcc
     1741 gccagttatc gcgcccatcg cgagctgcaa ggtatcctcg cctaagctgg cgcggcatcg
     1801 ggtctttcca agagtgcttc gacccccagg cgcgcgccgc ggctgcggcg tgctgaagcg
     1861 cgacctatgc ggacggcgca ttgctgcagc gcagcaaagg cccctacttt agttgcaagc
     1921 aaccaccaaa tcagcagctg cggacggccg ccgggcaggc gcgccggcgc gccgcgaagg
     1981 ccccgaccga caaggggccc cgacacggct ctggggggaa ggcacgaggt tggcacccag
     2041 aaagaggagc tgatccatga gcaccctg
//
LOCUS       DASTMT010000392         2023 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_6018202, whole genome shotgun sequence.
ACCESSION   DASTMT010000392 DASTMT010000000
VERSION     DASTMT010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2023
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6018202"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..631)
                     /locus_tag="VFR00_09195"
     CDS             complement(<1..631)
                     /locus_tag="VFR00_09195"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HEU0159479.1"
                     /translation="MRRVWMALLAAAITALCLWALLTPQVLQALGRLRSDAKPAPLLA
                     AFVLSGLVQWLRAWRFAVMTSARPTLPDAPMVLIALKLNFLNFVLPFRLGELGYPALM
                     HQQYGHGLLRSAGVLLLARLFDLTTVVAILLGAAALFLSTAVAQTAMLIAALGAALAP
                     FAMVGLGAALLPRLGLRPAAAPVKSSSSRPALAWPLGLRLGARLGARLRA"
     gene            complement(628..1626)
                     /locus_tag="VFR00_09200"
     CDS             complement(628..1626)
                     /locus_tag="VFR00_09200"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU0159480.1"
                     /translation="MTDTLSGMRTAAAARDTHALSIVIPAKDEEESLPLVVARIIAAC
                     RAGGHTLGDIVLVDDGSRDNTWQVMSKLAAEREVIQAIRLRRNFGKATALMVGIGACR
                     GDVIITMDADLQDDPDEISRFVEMLDRGYDLVSGWKKERHDPLNKTMPSRLFNKVTAT
                     ISGVKLNDFNCGFKAYRREIFDAIQLYGELHRYVPVLANALGYRIAEIPVRHHARRFG
                     ASKYGVARYLRGFLDLLTVVLITRFAYRPGHLFGGIGSLLALSGGVALSYLIGLKVLT
                     GASIGNRPLLLLGVLAVIVGVQLILFGMLAELIIHRTQRPVETRHLVAEQRGQPRA"
     gene            complement(1623..>2023)
                     /locus_tag="VFR00_09205"
     CDS             complement(1623..>2023)
                     /locus_tag="VFR00_09205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0159481.1"
                     /translation="GACRALVPFQDVAQHLQAAGFGGSGTIVVSDFHVGGNMRVQFPQ
                     ARLVEISYPPRVWPPPSGHGQCLVLWPAKGNVEVALDAYLRRDLGVPADARRREGELI
                     IPLPVLLPGAKTRAYRLFYRLYEESQGDCR"
BASE COUNT          403 a          664 c          665 g          291 t
ORIGIN      
        1 cggcccgcag gcgcgcgccc agtcgtgctc ccaaacgcaa tccaagcggc catgccaggg
       61 ccggccgtga tgagctcgat tttacgggcg ccgcagcagg cctgagccca agtcgcggca
      121 atagcgcggc gccgaggccc accatggcga agggagcaag cgcggccccc aaggcggcga
      181 tcagcatggc ggtctgcgcc acggcggtgc tcaagaaaag cgctgccgcg cccaacaaaa
      241 tggccaccac cgtcgtcaga tcgaacaatc gcgccagtaa gagcacgccc gccgagcgca
      301 acaggccatg gccgtactgc tggtgcatga gggcgggata gccgagctcg cccagccgga
      361 acggcaagac gaaattcagg aaattcagct tcagtgcgat caggaccatc ggcgcatcgg
      421 gtagcgtcgg ccgggcgctg gtcatcaccg cgaagcgcca ggcacgcagc cattgcacca
      481 ggcccgaaag cacgaaggcg gcgagcagtg gggcgggctt ggcgtccgag cgcaatcgcc
      541 ccagcgcctg caaaacctgt ggtgtcagca aagcccacag acagagcgcg gtgattgccg
      601 ccgccaacag cgccatccaa acccgcctca tgcgcgcggc tggcctcgct gctcggccac
      661 caggtggcgg gtctcgactg gccgctgggt gcgatggatg atcagctccg ccagcatgcc
      721 gaacaggatc agttgcacac cgacgatgac ggccaagaca cccaacagca acaggggtcg
      781 gttgccaatt gaggcgccgg tcagcacctt gagcccgatc aggtagctaa gggcaacacc
      841 gccgctcagg gccaataggc tgccgatgcc gccgaacagg tgtcccggtc gataggcaaa
      901 gcgggtgatc aaaactaccg tcaacaggtc gagaaagccg cgcaggtagc gggcgacgcc
      961 gtacttggag gcgccgaagc ggcgcgcatg atggcgcacc gggatctcgg cgatgcgata
     1021 accgagcgca ttggccagca cggggacgta gcgatgcagc tcaccataga gttgaatggc
     1081 atcgaatatt tcgcgtcggt aggccttgaa gccgcaattg aagtcgttga gcttcacccc
     1141 gctgatcgtc gccgtcacct tgttgaagag gcgcgagggc atggttttgt tgagcggatc
     1201 gtggcgctcc ttcttccaac ccgacaccaa gtcgtagcca cggtcgagca tctcgacaaa
     1261 gcgggaaatc tcgtcgggat catcctgcag atcggcgtcc atggtaatga tcacgtcgcc
     1321 gcggcaggcg ccgatgccga ccatcaaggc ggtcgccttg ccgaaattgc ggcgcagccg
     1381 gatcgcctgg atcacctcgc gctcggcggc gagcttcgac atcacctgcc aagtgttgtc
     1441 gcggctgcca tcatcgacca gcacgatgtc gcccaacgta tggccgccgg cgcggcaggc
     1501 ggctatgatg cgcgccacca ccaacggcag cgactcctcc tcatccttgg cgggaatgac
     1561 gatgctcaag gcgtgcgtgt cgcgcgccgc cgcggcggtc ctcatcccac tcaacgtgtc
     1621 ggtcatcggc agtcgccctg gctttcttca tacagtcgat agaacaggcg gtaggcgcgc
     1681 gtcttggcgc caggcagtaa aaccggcaag gggatgatca gctcgccctc acgccgacgc
     1741 gcgtcggccg gcacgcccag gtcgcggcgg aggtaggcgt ccaaggcgac ttcgacgttg
     1801 cccttggcgg gccacagcac caggcattgt ccgtgtccgg acggtggcgg ccagacgcgc
     1861 ggcgggtagc tgatctcgac cagccgcgcc tgcggaaact gcacgcgcat attgccgccg
     1921 acgtgaaagt cggacaccac gatcgtgccg gaaccgccga agccggcggc ctgcaagtgc
     1981 tgggcgacgt cctggaaggg aaccagcgcc cggcaggcgc ccg
//
LOCUS       DASTMT010000393         4155 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_5361800, whole genome shotgun sequence.
ACCESSION   DASTMT010000393 DASTMT010000000
VERSION     DASTMT010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4155)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4155)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4155
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5361800"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..251)
                     /locus_tag="VFR00_09210"
     CDS             complement(<1..251)
                     /locus_tag="VFR00_09210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4337 family protein"
                     /protein_id="HEU0159482.1"
                     /translation="MALDDVVERLSSDKDERNKWIGVYIGVLAVLLAICGVGGGNATK
                     DATRTNIEAANTWAFFQAKNMRRNAIQLAATELELSLAA"
     gene            complement(287..907)
                     /locus_tag="VFR00_09215"
     CDS             complement(287..907)
                     /locus_tag="VFR00_09215"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009466566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HEU0159483.1"
                     /translation="MDNVLVLVRHGESEWNKLNLFTGWKDPDLSEKGVAEAHKAGQLL
                     KAQGLHFDLAFTSVLKRAQRTLDIMLEELGQPGLTIRRDQRLNERDYGDLSGLNKDEA
                     RQRWGEKQVHIWRRSFDVPPPGGESLKDTAERVLPYYQSQIWPEVAAHRNVLVSAHGN
                     SLRALIMRLENLTGQEIVARELGTGVPIVYRLGADGHVCERRELAA"
     gene            complement(936..1763)
                     /gene="dapB"
                     /locus_tag="VFR00_09220"
     CDS             complement(936..1763)
                     /gene="dapB"
                     /locus_tag="VFR00_09220"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008833891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HEU0159484.1"
                     /translation="MAKLKIAVTGAAGRMGRELIRAVHANEGCVLAGAVEQAGSLALG
                     QDAGLLAGLGKLGVVIIDDPLELFTKIDAVLDFSAPAASLEFAALAANARIIHVMGTT
                     GMSAAEEAKVAAAARHATIIKAGNMSLGINLLSALVRKVAEALDADFDIEVLEMHHRH
                     KIDAPSGTALMLGRAAADGRQLRLEDVSVRVRDGHTGERRRGDIGFATLRGGSVVGDH
                     SVIFAADGERIELVHRAADRGIYARGAVKAALWARGKGPGLFNMMDVLGIESAKPRT"
     gene            1932..2501
                     /locus_tag="VFR00_09225"
     CDS             1932..2501
                     /locus_tag="VFR00_09225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0159485.1"
                     /translation="MRAAALLLGCMTGLGVTPAAANPVKSLYTTVELKACQPLKRLRD
                     GDAWRCQGLPGYPVYVAEGDLRQFVSAGKDAEHRRAATQTLQPFNSIFDGEHRRATIE
                     WRFERRGDGQIPYATIVRYHTSQQGRRGDVLVISKVTPLETCHLAYVDALANSNAIAL
                     ARRIADEMARAFDCSQEPRTLGAQGKSPM"
     gene            complement(2536..3045)
                     /locus_tag="VFR00_09230"
     CDS             complement(2536..3045)
                     /locus_tag="VFR00_09230"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU0159486.1"
                     /translation="MLGSGAKVRVRGGKPADAKALANVFRDSWLFAYRGIIPQRHLDS
                     MVRQRTPEWWRDAMKSGDSTLVLDVGGAVAGYATFGNSRQRGRYQGEIYELYLDPLYQ
                     GLGLGEHLFEGCRHALDMRRLDGLIVWALLGNSSACDFYWRRGGRPVASTFTRIGGER
                     LEKVAFAWG"
     gene            complement(3097..>4155)
                     /locus_tag="VFR00_09235"
     CDS             complement(3097..>4155)
                     /locus_tag="VFR00_09235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU0159487.1"
                     /translation="IRPDDIRRSRNGRLMRRLKFLVIDEVSMVRSDLMWAIDQSLRVN
                     RGRPREPFGGVRLALFGDLHQLPPVINEAEVAEHLETQHGGPFFFSLAALREGAGTAL
                     IELTQVFRQHDTALLAILNHIRDGAVGRADLAVLNERVRPIRTLAEGESYVILTPTNA
                     AASRINLAYLAALPGQASAYAAGISGEFNSAAHPTEEKLVLKPGAKVMLLRNDAERRW
                     VNGSIARIARLEEERVWIALDGAEHEVEQVAWENRRYAYDKVQEKIVETVAGTFRQFP
                     LRLAWALTIHKSQGLTLDHVYLDLGRGAFAHGQTYVALSRCRSLAGLALARPLRGEDI
                     LFDASVLGYRRLFEALIC"
BASE COUNT          746 a         1415 c         1282 g          712 t
ORIGIN      
        1 tgggcggcaa gcgaaagttc aagttccgtc gcggcaagct ggatcgcatt gcgacgcatg
       61 ttcttggctt gaaaaaaggc ccaggtattg gcggcctcga tgttggtacg cgtcgcatcc
      121 ttggtggcat tgccgccacc aacgccgcaa atcgccagca gtacggccag gacgccgatg
      181 tagacgccga tccacttgtt gcgctcgtcc ttgtcgctgc tgagcctctc aacgacgtcg
      241 tccaaggcca tgtcctcccc cttttcccga gccgccgggc aggcgcttag gctgcaagct
      301 cgcgccgctc gcagacatgg ccgtcggcgc ccagacgata aacgatcgga acgccggttc
      361 ccagctcgcg cgcaacgatc tcttgccccg tcaaattctc cagccgcatg atgagggcgc
      421 gcagcgaatt gccgtgcgcg gagacgagga cgttgcgatg cgccgcgacc tcgggccaga
      481 tctggctttg atagtagggc agcacacgct cggcggtatc tttgaggctt tcgccgccgg
      541 gcgggggaac atcaaagctg cgccgccaaa tatgcacctg cttctcgccc cagcgctggc
      601 gcgcctcgtc cttgttgagc ccggaaagat cgccgtagtc acgctcgttg agccgttgat
      661 cgcgccggat cgtcaacccc ggctgcccaa gctcttcgag catgatgtcg agggtgcgct
      721 gtgcacgttt gagcacgctg gtgaaggcaa gatcgaagtg aagaccctgc gccttcagca
      781 attgaccggc cttgtgcgcc tcggcaacgc ccttctcgct caggtcggga tccttccagc
      841 ccgtgaatag gttgagcttg ttccattcgc tctcgccgtg acgcaccagt acgagcacgt
      901 tgtccatccc tgtcctctgt cgagcgccct gccgctcacg ttctgggctt ggcgctctcg
      961 atgcccagca cgtccatcat attgaagagg ccgggtccct tgccgcgcgc ccagagtgcg
     1021 gccttgacgg cgccgcgcgc atagatgccc cgatcggcgg cgcgatgcac cagttcgatg
     1081 cgctcgccgt cggcggcaaa aattaccgag tggtcgccga ccacgctgcc gccgcgcaag
     1141 gtcgcaaaac cgatgtcgcc gcgtcggcgc tcgccggtgt gtccgtcgcg aacacgcacg
     1201 ctcacatcct caagcctaag ctggcgcccg tccgcggcgg cccgacccaa catcaatgcc
     1261 gtgccggatg gcgcgtcgat tttgtggcga tggtgcatct cgagcacctc gatatcgaaa
     1321 tcggcgtcca gtgcctcggc aaccttgcgc accagcgcgc tcaacagatt gatgccgagg
     1381 ctcatgttgc cggccttgat gatggtcgcg tgccgcgccg cggcggcgac cttggcctcc
     1441 tctgccgccg acatgcccgt cgtgcccatg acgtgaatga tgcgggcgtt ggcggcgagc
     1501 gcggcgaact ctagggaggc ggccggggcg ctgaaatcga gcaccgcgtc gattttggtg
     1561 aaaagctcca agggatcatc gatgataacc acaccgagct tgccgagccc cgccaacagg
     1621 cccgcgtcct gccccagagc cagcgagccg gcctgctcga cggcgcccgc caacacgcag
     1681 ccctcgttgg cgtgcacggc gcgtattaac tcgcggccca tgcgtcccgc ggcgccggtc
     1741 acggcgatct tcagctttgc cattacccac ctatctggct gtcacgcccc tatagcaaca
     1801 ccgcgcggcg ggcggaagcg gtcgaccccc gccgcgcccg cgagaaagac gtcagcgtcc
     1861 atggtatacg tgctcagccg ctgcgccgca aagaccggcg ctcggcggac ggtctggcag
     1921 gggaggccag catgcgtgcg gcggcgctgc tcttgggttg catgactggc ctgggtgtca
     1981 ccccagcggc cgccaatccg gtcaagagcc tctacacgac cgttgaactc aaggcctgtc
     2041 agccgctcaa gcggctgcgc gatggcgatg cgtggcggtg ccaaggcttg ccgggctatc
     2101 ccgtctacgt ggccgaaggc gatctgcgcc agttcgtcag cgccggcaag gatgccgaac
     2161 atcggcgcgc cgccacccag acgctgcaac ccttcaactc gattttcgac ggcgagcatc
     2221 gccgggccac gatcgaatgg cgcttcgagc gccgcggcga cgggcagatc ccctatgcca
     2281 ccatcgttcg ctatcacacc tcgcagcagg ggcgccgcgg cgacgtcttg gtgatttcca
     2341 aagtgacgcc cctggaaacc tgccacctcg cctacgtcga tgccctggcc aatagtaatg
     2401 ctatcgcgct cgcccggcgc attgccgacg agatggcccg tgcctttgat tgcagccagg
     2461 agccgcgcac cctgggcgcg caaggcaaaa gcccgatgta gccgttgcgg cggccgtgca
     2521 cgcgccttgc cgcgcttagc cccaggcgaa ggcgaccttt tccaggcgct cgccgccgat
     2581 gcgcgtaaag gtgcttgcga ccggacgccc gccacggcgc caataaaaat cgcaggcgct
     2641 gctattgccg agcagcgccc agacaatcag gccatcgagc cggcgcatgt cgagcgcgtg
     2701 acggcagccc tcgaacaggt gctcgccaag acccaaacct tgataaagcg gatcgagata
     2761 gagctcgtag atctcgccct ggtagcgtcc gcgctggcgc gagttgccga aggtggcata
     2821 gcccgcgacc gccccaccca cgtccaaaac caaggtactg tcaccgctct tcatggcgtc
     2881 gcgccaccac tccggcgtgc gctggcgaac catgctgtcg aggtggcgtt gcggaatgat
     2941 gccgcggtag gcgaacagcc aagagtccct gaaaacgttg gcgagcgcct ttgcatcggc
     3001 aggcttgccg ccgcgcaccc gcacctttgc acctgagccc agcatgccga cactatagcg
     3061 cggccggcat cgctgtgatc cagaaatttt tgccgctcag caaatcagcg cctcgaacag
     3121 gcgccgatag ccgagcaccg aggcgtcaaa caaaatatcc tccccgcgca gcggccgggc
     3181 cagcgccagc cccgccaggg agcggcagcg cgaaagggca acataagtct ggccatgagc
     3241 aaaagcgcct cgccccagat cgaggtagac atggtcaagc gtcaacccct gacttttgtg
     3301 aatggtcaag gcccaggcca gacgcaaggg aaactgacgg aaggtccccg ccacggtctc
     3361 gacgatcttt tcctgcacct tgtcataggc ataacgccgg ttttcccagg cgacctgctc
     3421 cacttcatgt tccgcaccgt cgagcgcaat ccacacgcgc tcctcctcga gccgggcgat
     3481 gcgggcgatc gagccattga cccagcggcg ttcggcatcg ttgcgcaaca gcatgacctt
     3541 ggccccgggc ttcagcacaa gcttctcctc ggtggggtgt gcggcactgt tgaactcacc
     3601 gctgatgccc gccgcatagg cgctggcttg ccccggcaag gcggcaagat aggcgagatt
     3661 aatgcggctc gccgccgcgt tggtgggcgt caggatgacg taggattcgc cttccgccaa
     3721 ggtgcggatc gggcgcaccc gctcgttcag cacagcaaga tcggcgcggc caaccgcacc
     3781 gtcgcggata tgattgagga ttgcgagcag cgccgtatca tgctggcgga agacctgcgt
     3841 caactcgatc aacgctgttc ccgctccctc gcgcagcgcc gccaaggaaa agaaaaaggg
     3901 ccccccgtgc tgtgtttcca ggtgctcggc gacctcggct tcgttgatca cgggtggcag
     3961 ctgatgcagg tcgccaaaga gcgccaggcg cacgcccccg aacggctcgc ggggtcgccc
     4021 gcgattgacg cgcaaggact ggtcgatcgc ccacatcaga tcggagcgca ccatggagac
     4081 ttcgtcgatc acgagaaact tgagccgtcg catcagtcgg ccgttacggc tgcgccgaat
     4141 gtcgtccggg cggat
//
LOCUS       DASTMT010000394         1554 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_875273, whole genome shotgun sequence.
ACCESSION   DASTMT010000394 DASTMT010000000
VERSION     DASTMT010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 1554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1554
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_875273"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            11..271
                     /locus_tag="VFR00_09240"
     CDS             11..271
                     /locus_tag="VFR00_09240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEU0159488.1"
                     /translation="MPRITYIEHDGTSHTVEAECGGACACATCHVYVEEAWRDKVGPP
                     SPMEEDMLDFALEVKESSRLSCQIKVKPELDGLVVRVPEKQF"
     gene            434..847
                     /locus_tag="VFR00_09245"
     CDS             434..847
                     /locus_tag="VFR00_09245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0159489.1"
                     /translation="MRTSWLSSAVILALALLGGGALFHAGAAQEKRWAAIDPNNETTS
                     PVAWSSTEAKARQDAIEACKRTSKSCANGPASTNEMEDVFAIMCCTKPRRGCAVGVAG
                     ARQEALKSVQKTFADAGYSNCSLRHYLSAATGKKD"
     gene            909..>1554
                     /locus_tag="VFR00_09250"
     CDS             909..>1554
                     /locus_tag="VFR00_09250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002712655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEU0159490.1"
                     /translation="MPAEGSSGALPIETDALIIGAGPVGLFAVFELGLVDIKAHVVDI
                     LDKIGGQCAELYPEKPIYDIPGLPIVTGQGLTDALLAQIKPFGAQFHLNRRIDALTRA
                     PDGRFRLDTDAGEQFLAKVVVVAAGGGSFTPKRPPLAGIEAHEGTSVFYSVRRMEDFR
                     DKDVLIVGGGDSALDWTLSLAPLARSLTLLHRRDEFRAAPHSVEQMRALVAANKV"
BASE COUNT          266 a          493 c          513 g          282 t
ORIGIN      
        1 aagccaggcg atgcccagga ttacctacat cgagcacgac ggcacgtcgc acaccgtcga
       61 ggccgaatgc gggggcgcct gtgcctgcgc gacctgccac gtctatgtcg aggaggcctg
      121 gcgggacaag gtcgggccgc catccccgat ggaagaggat atgctcgatt ttgcgttgga
      181 ggtgaaggag tcgagccggc tcagctgcca aatcaaggtg aaaccggagc tcgacggctt
      241 ggttgtacgc gtaccggaaa agcagttcta gccgccccgg ctcggcagcc cgggtgccgg
      301 cccgcttgcc aggctgcttg accagaccgc tgggccagac tgctgggcga gactgctgga
      361 ccagactgct tcgcgagcct gcggcctcag cgccagctct ggcataccgt gagccgattg
      421 ttggagtacg ccgatgcgca catcctggct tagctcggcg gtgattcttg ccctggcact
      481 gctggggggc ggtgcgcttt tccacgctgg tgcggcccaa gagaaacgct gggctgccat
      541 tgaccccaac aacgaaacga catcgcccgt ggcctggtcg agcaccgagg ccaaggcgcg
      601 gcaggacgcg atcgaggcct gcaagcgcac ctccaagagc tgcgccaatg ggccggcctc
      661 gaccaacgag atggaggacg tgtttgccat catgtgctgc acaaagccgc gccggggttg
      721 cgccgtgggt gtcgcagggg ctaggcagga ggcgctaaaa agcgtgcaga agacctttgc
      781 cgatgccggc tattccaatt gttcgttgcg tcactatctc tcggcggcga ccggcaaaaa
      841 ggattaaatg cctgccggcc gactgaacct tggcgcttgt ggcgctctgc tatcgggatc
      901 tgacctccat gccagctgaa ggctctagcg gcgccctgcc gatcgagacc gacgccctca
      961 ttatcggtgc cgggccggtg ggcctgttcg ccgtgttcga gttgggcctg gtcgatatca
     1021 aggcgcacgt ggtcgatatt ctcgacaaga tcggcggaca atgcgccgag ctttatccgg
     1081 aaaagccgat ttacgacatt cccggcttgc cgatcgtgac cgggcagggc ctgaccgatg
     1141 cgctgctggc ccagatcaaa ccgttcggtg cccagttcca cctcaatcgc cgcatcgatg
     1201 ccctgacgcg cgcgccggac ggccgctttc gtctcgacac cgacgcgggc gagcagttcc
     1261 tcgccaaggt ggtggtggtg gcggccggtg gcggctcctt tacgcccaag cgcccgcccc
     1321 ttgccggcat cgaggcgcac gaaggcacct cggtgttcta ttcggtgcgg cgcatggaag
     1381 atttccgcga caaggatgtc ttgattgtcg gtggcggcga ttcggcgctc gactggacgc
     1441 tcagtcttgc gcccctggcc cgcagcctga ccctgcttca ccgccgcgac gagtttcgcg
     1501 ccgcgccgca ttccgtcgag cagatgcgcg cgctggtggc cgccaataag gtca
//
LOCUS       DASTMT010000395         2442 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_2510169, whole genome shotgun sequence.
ACCESSION   DASTMT010000395 DASTMT010000000
VERSION     DASTMT010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2442)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2442)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2442
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2510169"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..870)
                     /locus_tag="VFR00_09255"
     CDS             complement(<1..870)
                     /locus_tag="VFR00_09255"
                     /inference="COORDINATES: protein motif:HMM:NF015210.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HEU0159491.1"
                     /translation="MSTYPMIGNFSSVTVPLGQLFTNPISIEAPGYQRSFAWTADEAG
                     QLFEDIRQALAEESDYFLSFMLFIERNRPRSRLSAWSLGRSDRVLEIVDGFQRLTSLT
                     ILFCALRDLDAEQGQPTNARLEAAIGRPGRPRLSLRPAEATFFEQYVRLPGATRIKPA
                     AANLTPAEARIAWVRDHFVAELADMAAADRPFFIDFLLDHCCVASVVTSTIDRAHQLF
                     TILNARGKPLGRKDILKATLLGGVPADAAARAATLWQDLDQRLGDELENLFSHVRAMY
                     GRTSAHVIAGIKDI"
     gene            1018..2118
                     /locus_tag="VFR00_09260"
     CDS             1018..2118
                     /locus_tag="VFR00_09260"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020088003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HEU0159492.1"
                     /translation="MFGALFDLARPFLHSLDPEQAHELTLKALECGLYPRAGGRNDPR
                     LAIELWGLRFSNPFGLAAGFDKDARVADALLAMGFGFTEVGTVTPRPQAGNPRPRVFR
                     LSSDRAIINRLGFNSAGHAAVHARLAGRPQGVGIVGVNVGANRDAADRASDYVAGIHC
                     FSEVAAYLTINVSSPNTPHLRDLQAPAQLDALLGRLAEARREQVAAGKPRRPIVVKLA
                     PDIAEADLPGIVEVLVARAVDGIAVGNTTLQRTGLTDARQSAEQGGLSGRPLFMRSTI
                     VLARVHQLARGAMPLIGVGGIDSGRTAIAKIEAGASLLQLYTGLVYEGPTLLARLQQE
                     LAAYLEHGGHARLADAVGCRAAAWAAKPLPAV"
     gene            2253..>2442
                     /locus_tag="VFR00_09265"
     CDS             2253..>2442
                     /locus_tag="VFR00_09265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0159493.1"
                     /translation="MLTQLAVLAAAGAGFLNAPWWFWIVAGVALAVLGLTDPDKVHPR
                     YADGGIAQLLVVDSLARLS"
BASE COUNT          419 a          787 c          798 g          438 t
ORIGIN      
        1 gatgtccttg atgccagcga tcacatgagc gctggtgcgt ccgtacatgg cgcgcacgtg
       61 gctgaagagg ttctcgagtt cgtcgcctaa gcgttgatcg aggtcctgcc agagcgtggc
      121 cgcgcgcgcg gcggcatccg caggcacccc gcccagcagc gtggccttta gaatatcctt
      181 gcggccgagg ggtttgcccc gggcgttgag aatcgtgaac agttgatggg cgcgatcgat
      241 cgtgctggtg acgacggagg ccacgcaaca gtgatcgagc agaaagtcga taaaaaaggg
      301 tcggtctgcc gccgccatat cggcaagctc ggccacaaag tgatcgcgca cccaggcgat
      361 gcgcgcctcc gcgggcgtca gattggcggc ggcgggtttg atccgcgtgg ccccggggag
      421 ccgcacatac tgttcgaaaa acgtcgcctc ggctggccgc agggacaggc gcggccgtcc
      481 cggtcggcca attgcggcct ccagccgcgc attggtgggc tggccctgct ccgcatccag
      541 gtcgcgcagg gcacaaaaca aaatggtgag cgaggtcagc ctctgaaaac catcgacaat
      601 ttccagtacc cggtcactgc gcccgagcga ccaggccgac agccgggagc gcggccgatt
      661 gcgttcaatg aacagcatga agctgagaaa atagtcggac tcctccgcaa gcgcctggcg
      721 gatatcctca aacagctgtc ctgcttcgtc ggccgtccag gcgaaggagc gctgatagcc
      781 cggcgcctcg atggaaattg gattggtgaa aagttggccg aggggcaccg tcacggaaga
      841 gaaattgcca atcatggggt acgtgctcac gggctcggtt cgcccaattg tcagcatgtt
      901 tcaagccgta cttgcaattg ccaacgtgca gttgcgctga gcgccgtttt gtgctggacg
      961 aagtggatac gcacagatct ccaaagctca taaggcgcgc aacggaaacg aaaaaatatg
     1021 tttggcgccc tcttcgacct cgcgcgaccg ttcctgcata gcctcgatcc cgagcaggcg
     1081 catgagctta ctcttaaggc actggaatgt gggctttatc cgcgtgcggg ggggcgcaac
     1141 gatccgcgcc tggcgatcga gctctgggga ctgcggtttt ctaatccctt cggcctcgcc
     1201 gccggtttcg acaaggatgc ccgcgttgcc gatgcgctcc tggcgatggg gtttggcttc
     1261 acggaagtcg gcaccgtcac gccgcgtcca caagccggca acccgcgccc gcgtgtcttc
     1321 cggctctcga gcgaccgggc catcatcaac aggttaggct tcaacagtgc cggacacgcc
     1381 gccgttcatg cacgcctggc cgggcgccct cagggtgtcg gcatcgttgg cgtcaacgtc
     1441 ggagccaacc gggatgcggc ggatcgtgcc tccgattatg tcgccgggat ccactgcttt
     1501 agcgaggtcg ccgcctatct gaccatcaac gtctcttcac ccaacactcc gcatctgcgc
     1561 gatctgcaag cgccggccca actcgacgcg ctattgggac gcctcgcgga ggcacgccgg
     1621 gagcaggtcg cggccggcaa accacggcga cctattgtcg tgaagctggc accggacatt
     1681 gccgaggccg acttgcccgg catcgtcgag gtgctggtgg cgcgagccgt cgacggcatt
     1741 gccgtgggca acacgacgct gcaacgcacg gggctgaccg atgcgcggca gagcgccgag
     1801 cagggtgggc tttccggtcg cccgcttttc atgcgctcaa ccatcgtgct ggcccgggtg
     1861 catcagcttg cgcgcggcgc catgccgctg atcggcgttg gcggcatcga ttcgggacgt
     1921 acggccatcg ccaagatcga agccggtgcc agcttgctgc aactctatac ggggctcgtc
     1981 tacgagggcc cgacgctgct tgcgcgcctg cagcaagagc tcgccgccta ccttgagcat
     2041 ggtggccatg cccgcctggc agatgccgtg ggatgccgtg ccgcggcgtg ggcggccaag
     2101 cccctgccag cggtgtaggc gcccggccgc aggccacggc tgcgacagcg gctcggcgtc
     2161 cccaatttcc ccagttgcaa gttggccgct ttaccgggac cgccgactcg gcggaaagac
     2221 gccgccgcac gcttgatcga ccggaggcct caatgctcac tcagctcgcc gtgcttgccg
     2281 ccgccggcgc gggtttcctc aacgcgccgt ggtggttttg gatcgttgcg ggcgtggcgt
     2341 tggccgtgct cgggctcacc gatcccgaca aagtccatcc gcgctacgcg gacgggggca
     2401 tcgctcagct ccttgtggtc gacagcctcg cgcgtctttc ca
//
LOCUS       DASTMT010000396         3142 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_4154810, whole genome shotgun sequence.
ACCESSION   DASTMT010000396 DASTMT010000000
VERSION     DASTMT010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3142
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4154810"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            68..1309
                     /locus_tag="VFR00_09270"
     CDS             68..1309
                     /locus_tag="VFR00_09270"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013417933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEU0159494.1"
                     /translation="MRAIGLAAREAAAALAQASGEAKRAALSAAGREIRAQTAAILSA
                     NERDLKEARRAEANAAFLDRLLLDPQRVEAIATGLDQVSGLADPVGAVLARWTRPNGL
                     RIERVRVPLGVVGIIYESRPNVTADAGGLCLKSGNAAILRGGSDSHHSSRAIQVCLAR
                     GLREAGLPESAVQLVPTTDRQAVGLMLKGLDGAIDVIVPRGGKSLVARVQEEARVPVF
                     AHLEGICHTYVDRSAELGMAKAIILNAKLRRTGVCGATETLLVDTAAPAGHLAALVGA
                     LADAGCEVRGDAAAQAADKRVLAASEQDWSAEYLDAIIAVRAVDGVDGAIDHIRRYGS
                     QHTDCIVADDSIAAECFLAGLDSAIVLHNASTQFADGGEFGMGAEIGIATGRLHARGP
                     VGVEQLTTFKYVVRGNGQIRP"
     gene            complement(1380..1628)
                     /locus_tag="VFR00_09275"
     CDS             complement(1380..1628)
                     /locus_tag="VFR00_09275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019861233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEU0159495.1"
                     /translation="MDPQALIIFLIIGAIAGWLAGMVMTGGGFGLIGNIIVGIIGAFV
                     AGWLFPNFWPLGGILGPILHAAIGAIIVLFVVGLIKRA"
     gene            1885..3069
                     /locus_tag="VFR00_09280"
     CDS             1885..3069
                     /locus_tag="VFR00_09280"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018458263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEU0159496.1"
                     /translation="MAGVLEGVRVLDFGRYIAGPYCAALLAEHGAEVIRIEKRDGSED
                     RYQAPVASSGEGALFLQMNRNKLGMTLDPMRPEGQEVVRRLLERADVVIANLPPQTLV
                     AMKLDYASLTQVKPDIILTTVSAYGRGGPYSDRVGFDGIGQVMSGAVYMTGEEDQPYR
                     AQVPWVDFGTALHCAFGTMAALLARRVSGKGQWVEGALLATAVTFSNALLIEQAVIAP
                     NRVPTGNRGQTSGPVDIFRTKDGWILVQVVGNPLFARWARLMGDEHWLSDPRFKDDIS
                     RGDNGAVISERMRRWAAAYSNAEALELLARAKIPAGPVLKPQATLEDPHINAVGFFQP
                     TEFPGAPRPAPIAKVPVWLSETPGSIRRRAPRLGEHTDQILAELGYNNSAIAALRDKG
                     VI"
BASE COUNT          562 a         1062 c         1040 g          478 t
ORIGIN      
        1 agcaacgccc gcaacgccct tgtcgacgca acgcaaaccc gaaacgctcg acgatgtcgc
       61 tgcggcgatg cgggccatcg gccttgccgc gcgcgaggcg gcagccgcct tggcgcaggc
      121 cagcggcgaa gccaagcgag cggcgctgag cgcggccggg cgcgagataa gagcgcaaac
      181 cgccgccatc ctgagcgcca acgagcgcga cctcaaggag gcgcgccgcg ccgaggcgaa
      241 cgcagccttt cttgatcgcc tcctgctcga tccccagcgc gtcgaggcca tcgccacagg
      301 tttggaccag gtctccggcc tcgccgatcc cgtcggcgcc gtgctcgccc gctggacccg
      361 tcccaatggg cttcgcatcg agcgcgtgcg cgtgccgctg ggcgtggtcg gcatcattta
      421 tgaaagccgc ccgaacgtga ccgccgatgc cggtgggctt tgtctaaagt ccggcaacgc
      481 cgcaattctg cgcggcggtt cggacagcca tcactccagc cgcgccatcc aggtctgcct
      541 ggcgcgcggc ctgcgcgagg ccggactgcc cgagagcgcg gtccagctcg tgcccaccac
      601 cgaccgccaa gccgtcgggc tgatgttgaa gggtctcgac ggtgccatcg acgtgatcgt
      661 gccgcgcggc ggcaaaagcc tggtggcgcg cgtgcaggag gaagcgcgcg tgccggtatt
      721 tgcgcatttg gaaggcatct gccacaccta cgtcgaccgc agcgccgagc tgggcatggc
      781 caaggcgatc atactcaacg ccaagctgcg gcggaccggt gtctgcggcg cgaccgagac
      841 cctgctcgtc gacacggcgg cgcccgccgg ccacctggcg gccctggtcg gcgcccttgc
      901 cgatgccggc tgcgaggtca gaggcgatgc cgccgcgcaa gccgccgaca agcgtgtgct
      961 ggctgccagc gaacaagact ggtcggccga atatctcgat gccatcattg ccgtgcgcgc
     1021 cgtcgacggc gtcgacggcg ccatcgacca cattcgccgc tatggctcac agcacaccga
     1081 ttgcattgtc gccgacgaca gcatcgccgc cgagtgcttt ctcgccggcc tcgacagcgc
     1141 catcgtgttg cacaatgcct ccacccaatt cgccgacggt ggcgaattcg gcatgggcgc
     1201 ggaaatcggc atcgcgaccg ggcgcctgca tgcccgcgga ccggtcggcg tcgagcagct
     1261 gaccaccttc aaatacgtgg tgcgcggcaa cggtcaaatc cggccgtgac ggcggcagcc
     1321 gccggcgcag acgaggcgcg cacgtccgct taaagacggc cgcgcgcgcc gcgcccaatc
     1381 taggcgcgtt tgatgaggcc aaccacgaat agcacgatga tggcgccgat ggccgcatgc
     1441 aggatgggcc cgaggatgcc cccgagcggc cagaaattgg ggaacagcca cccggcaacg
     1501 aaggcaccga taatgccgac aatgatgttg ccgatcaggc cgaagcctcc ccccgtcatc
     1561 accatgccag caagccaacc ggcgatggcg cctatgatca aaaagatgat gagtgcctgc
     1621 ggatccatgc tgttgctccc ggatgtggga ggacagggca aaggcctgcc cttcccgtgt
     1681 tgccaaatcc cgcaaacttg aacccgtacg atcgtcagca cgccgcaggc taaactgctt
     1741 tgccgtccag cgccatcgtt cagcgcgggt gtgggccgcg ccgaccgcga gctcgatgca
     1801 gcgcacaaga ggcagccaag ttgtgacgat cctgatagac atagggcgag gccggttctg
     1861 tcatggaaag caaagggttt gcaaatggct ggtgttctcg agggcgttcg tgtgctggat
     1921 ttcggccgct acattgcggg gccctactgc gccgcgctct tggccgaaca cggcgccgag
     1981 gtgatccgca tcgaaaagcg cgacggcagc gaggaccgct atcaggcccc ggtggccagc
     2041 agcggcgagg gtgccttgtt cctgcagatg aaccgcaaca agctcggcat gacgctcgat
     2101 cccatgcgac cggaaggcca agaggtcgtg cgtcgcctgc tcgagcgggc cgatgtggtg
     2161 atcgctaatc tgccgccgca gaccctggtg gccatgaagc ttgactacgc cagcttaacg
     2221 caggttaagc cggacatcat tctgacgacc gtctccgcct acggccgcgg cgggccctac
     2281 agcgaccgcg tcggcttcga cggcatcggc caggtgatgt ccggcgcggt ctacatgact
     2341 ggagaggaag accagcccta ccgcgcgcag gttccctggg tcgattttgg cacggctctg
     2401 cactgtgcct ttggcaccat ggcggcgctg ctggcgcggc gggtcagcgg caaaggccag
     2461 tgggtggaag gcgccctgct ggcgacggcg gtcacgttca gcaacgccct gttgatcgaa
     2521 caggccgtaa tcgcccccaa tcgcgtgccg accggcaatc gagggcagac atcggggccg
     2581 gtcgacattt tccgcaccaa ggacggctgg atcctcgtgc aggtggtcgg caacccactg
     2641 tttgcgcgct gggcccgcct gatgggcgac gagcattggc tgagcgaccc tcgcttcaag
     2701 gacgacatct cgcggggcga caacggcgcc gtgatcagtg aacgcatgcg ccgctgggcg
     2761 gcggcctaca gcaatgccga ggcgctggag ctgttggcca gggcaaaaat tccagccggg
     2821 cccgtgctca agccgcaagc aacgctggag gatccccaca tcaacgccgt cggctttttt
     2881 cagccaaccg aattccccgg cgcgccgcgg ccggcgccga ttgccaaggt gccggtgtgg
     2941 ctgtctgaaa ccccgggctc gattcgccgc cgcgcaccgc ggctgggcga gcatacggac
     3001 cagatcctcg ccgagctcgg ctacaacaac agcgccattg ccgcccttcg cgacaagggc
     3061 gtgatctgac gcggcggccc tttagcggtg ccggttcgcc gccacaggcc aggaggcgag
     3121 catgagcgaa gcaatcggca ac
//
LOCUS       DASTMT010000397         5629 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_877414, whole genome shotgun sequence.
ACCESSION   DASTMT010000397 DASTMT010000000
VERSION     DASTMT010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 5629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5629
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_877414"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(709..2031)
                     /locus_tag="VFR00_09285"
     CDS             complement(709..2031)
                     /locus_tag="VFR00_09285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU0159497.1"
                     /translation="MLRDAQQHDLSGATPEAITSFDAAVRAFTLVYGDTLGLYEAARA
                     AAPNFVMAHLGKAWVLSLASDPILAQEAKRLIDGAAALARNQRENAHFAALSHAVAGH
                     RAAAVAVLDRHLMAYPFDLLAHMAALQMDGHLGRFPLARARSARALPHWSKAQEGYGI
                     LLSFFAFGLEEAGDYARAEEVARAAAELEPFGYWPHHCVSHVMEMTGEPQLGLDWMTE
                     RAPFWSAKDNNNRVHIWWHKALFHIELGQYAAALEIYDGPILGAMRPASISICNASSL
                     LWRLELLECDVGDRWQQLAARWPGHANGRLYVFCDIHAAMAELRAGQADALEQRLAAM
                     RLTAADHSEAAPLYRDVGLPLIEALAAFHHGAYADAVERLLPVRFELWKMGGSHAQRD
                     VIDFTLAEAAARAGWRDIALALAHERLGTRPQSAPNRRFLAQAQTIPA"
     gene            complement(2309..2656)
                     /locus_tag="VFR00_09290"
     CDS             complement(2309..2656)
                     /locus_tag="VFR00_09290"
                     /inference="COORDINATES: protein motif:HMM:NF013014.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H-NS histone family protein"
                     /protein_id="HEU0159498.1"
                     /translation="MARSNGRLSEKQVSAWFAELDFDKQSAILGSLHATHDSAKQARI
                     DALRRQLSDLEGARARRAAGKGARKGRRRKGPAAVKYRDPKTGDTWSGRGRMARWLAA
                     KVSAGEKADKYLA"
     gene            complement(2820..3458)
                     /locus_tag="VFR00_09295"
     CDS             complement(2820..3458)
                     /locus_tag="VFR00_09295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0159499.1"
                     /translation="MSAEELLSATWRSLAGRQGASASAAEAVRDELMRAYGEPTRQYH
                     TLEHIAALLQQWEAHKATLRDADAVALAILFHDVVYDPRRQDNEQQSAAIARQRLTPL
                     GFAPSLIGRVVAYIEATQHGREIDSEDPDLALLLDLDLSTLAAAPDAYRLYASAIRRE
                     YAHVPEALYRFGRRQVLEGFLARSQIYRTPQLAALWEARARANISAEIASLA"
     gene            complement(3476..4462)
                     /locus_tag="VFR00_09300"
     CDS             complement(3476..4462)
                     /locus_tag="VFR00_09300"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEU0159500.1"
                     /translation="MPRSLAQGCRRLVLAAAAATFAAGVPAAAQDVYPSQTVKFVVAF
                     PAGGPTDAMARILGQRLAEKWGQGVVIENRAGAGGNIAARQIAKAEATGYSVLVTTSA
                     FAVNPSLTSNAGYSPETEFKTAIVAATTPNILVASKTLKASTLKDLIEAARSEKFTYG
                     MPGPGTTPHLSAERIFKSLAKVDIPPVSFTGAAPLLNALVGGHIMLACLAMPPAIELI
                     KSGEIKGLAVLSDKRVPTLPQVPTAIELGYGDSEESTWIALFVPAATPPAVLAKLNAD
                     ANAVLAEAPIRERLEPLGLLPVGGSPETSEAYVRAEIKKWGEVVRNLGLKLE"
     gene            4606..5532
                     /gene="kdgD"
                     /locus_tag="VFR00_09305"
     CDS             4606..5532
                     /gene="kdgD"
                     /locus_tag="VFR00_09305"
                     /EC_number="4.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007817093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047448 - 5-dehydro-4-deoxyglucarate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxyglucarate dehydratase"
                     /protein_id="HEU0159501.1"
                     /translation="MTPDLAGLQKTLGSGLLAFPATAFDAALDFDEETYRESIALNIG
                     HGAVGLFAPGGTGEFFSLTPEECRRVTRAAVVAAAGRVPIVAGTGYGTALAVEIAKAA
                     EAAGAQAVLVLPPYLINAEPSGLEAHVDAICRAVGIGVILYNRDNSLYAAESVARLAE
                     RHANLIGFKDGHGDVEQLVRVRRLLGNRLLYIGGMPTAEVFAVPYFAAGFTTYSSAVY
                     NFIPETAQRFYAAVKAGDTRTCDDLLSSFFIPYIALRNRKRGYAVAIVKAGMRLLGRS
                     AGPVRPPLTDLTPAEMDEFKTLLERAFGGRAR"
BASE COUNT          936 a         1786 c         1914 g          993 t
ORIGIN      
        1 cgcgcctttt tgcccggcag cgtacgccgt atggttaaca ccgatcgccg tcgggctcca
       61 gcgttgttga taagccgtgt ggaaaggacg cgaccggact aaggcaggcg agggcggccg
      121 acatgtgcct tggctctggg atggaatggt cgcgagcgtg ccgcgcgggc gcgctccgct
      181 gccgctaaaa gcctgaaatg acaggaagtc gtgggtttgc gggaaaggcg cgctcagggc
      241 agcggcgaga tgcaaggcga gaagaagagg actgcgccat gtcgcagccc tccaagtgca
      301 ataagctaaa caattgggag cggactagag ccctcagcag ccccgaccgc ggtgcggggt
      361 tgtggtcgag gcgcgcgggc gaaacgccag ctccagttgc ccagcatacg cgaaaaatct
      421 ccttggcgac gtggtgaggc gcaaaaaaaa agggttttgc tcacccccta gacaagcgcc
      481 cgatctctgt tgcggtggga cgttgtggga cgatattaat tttctatctg aggacgcgcg
      541 accccgcgcg cggtgccagg gctcggtggc gatcgccgct gcagcccaac gcggtcaacc
      601 ctcgcgaatg cgccagcgat tgggcctctc cgatcgctgc atcgcctcga ggtggtgcgc
      661 gatctccaca atggcccgcg acgttgtcgc ggcgcggccc acgcgttttc aggccgggat
      721 ggtctgggct tgggcgagaa agcggcggtt gggcgcgctt tgcggccgcg tgcctagccg
      781 ttcatgggcc agcgccagcg cgatatcgcg ccagccggcg cgggccgcgg cttcggccaa
      841 ggtgaaatcg atcacgtcgc gttgcgcgtg actgccgccc attttccaga gctcaaagcg
      901 gaccggtagc aggcgctcga cggcatctgc ataggcgccg tgatggaagg cggcgagcgc
      961 ttcgatgagc ggcaagccga cgtcgcgata gagcggggcg gcctcggagt gatccgccgc
     1021 cgttaagcgc attgccgcca agcgctgctc gagcgcgtcg gcttgcccgg cccgcagttc
     1081 tgccatcgcc gcatggatgt cgcagaacac gtagagccga ccgttggcgt ggcccggcca
     1141 gcgcgccgcc agctgctgcc agcgatcgcc cacgtcgcac tccaacagct cgagacgcca
     1201 gagcagggag gacgcattgc agatgctgat gctggccggg cgcatcgcgc cgaggattgg
     1261 gccatcgtag atttccagcg cggccgcata ctggccgagc tcgatgtgga atagggcctt
     1321 gtgccaccag atgtgcacgc gattgttgtt gtccttggcc gaccagaagg gtgcccgctc
     1381 ggtcatccaa tcgagcccca attgcggctc accggtcatc tccatcacgt gcgagacgca
     1441 atggtgcggc caatagccga agggctcgag ctcggcggcg gcgcgcgcca cctcctcggc
     1501 tcgggcgtag tcgccggctt cctcgaggcc gaaggcgaaa aacgacaaca agatgccgta
     1561 gccctcctgc gccttggacc agtgcggcag cgcccgcgcc gagcgcgccc gcgccagggg
     1621 aaagcggccg agatggccat ccatctgcag cgccgccatg tgcgccagga gatcgaaggg
     1681 ataggccatg agatggcggt cgagtacggc aacggcggcc gcgcggtgcc cggcaacggc
     1741 atgcgacagg gcggcgaaat gggcgttctc gcgttgattg cgcgcgagcg ctgcggcgcc
     1801 gtcgatgagg cgcttggcct cttgcgccag gatcggatcg ctggccagcg acagcaccca
     1861 ggccttgccg agatgcgcca tgacgaaatt gggcgcggcg gcacgggcag cctcgtagag
     1921 gccaagcgtg tcgccgtaga ccagggtgaa ggcgcgaacc gcggcgtcga aagacgtaat
     1981 cgcttcgggc gtggccccgg acaggtcatg ttgctgggcg tcccgcaaca tggggcttgg
     2041 ctccttcagc tgagcccgga ttccccaccc gagctcaccg ccttcgcgtt ggcaaggctc
     2101 gggtggggca gtgtttcggc aaggttagtc gattgcgccg gggtgcgcga ggggcgccga
     2161 atctcggaca aaagaaaggg gccgcttcgg agggaacggc ccctttccat cctgcaccgc
     2221 aacgtgtggg agaacacacg aacggcagcc tagcccaacg ccgtgacgtt gtcacccccc
     2281 aggctgtgtg taacttgaaa ccggcgtctt aggccaagta cttatcggct ttttctccgg
     2341 ccgagacttt tgcggcgagc cagcgcgcca tgcggccgcg gcccgaccag gtgtcgccgg
     2401 ttttcgggtc gcggtacttg acggcggccg gacctttccg acggcggcct ttgcgggcgc
     2461 ccttgccggc ggcgcgccgg gccctggccc cttccaggtc ggagagctgg cgacgcagcg
     2521 cgtcgatcct cgcctgcttg gcgctgtcgt gcgttgcatg cagggaaccg agaatcgcgc
     2581 tctgcttatc gaagtccagc tccgcgaacc aagcagagac ctgcttttcg gaaagcctac
     2641 cgttggaccg tgccatcgtt cctactcgtc aagactgttg aggacccgtg ttttatcgat
     2701 aatggtatgt cgatgcaatt tattagatag catggttgtt ttgttattcg tgcacggatg
     2761 aatctgtatt gcctcaaaaa aagattatgt attatcgggt gttgcccgag cggcgcacct
     2821 cacgccaggc ttgcaatctc cgcgctaata ttggcgcggg cgcgcgcttc ccaaagcgcg
     2881 gcgagttgcg gggtgcggta aatctggctg cgggccagaa atccctccag cacttgccgg
     2941 cggccgaagc gatagagcgc ctcgggcacg tgggcatact cgcgccgaat ggcgctggcg
     3001 tagagccgat aggcatccgg tgcggccgcc agcgtcgaca gatcgaggtc gagtagcagc
     3061 gccagatccg gatcctcgct atcgatctcg cggccgtgtt gggtcgcctc gatataggca
     3121 acgaccctgc cgatcaggct tggggcaaag ccgagcgggg ttaggcgctg gcgcgcgatg
     3181 gctgcgcttt gctgctcgtt gtcttggcga cgcgggtcgt aaacgacatc atgaaaaaga
     3241 atggccagcg ccacggcgtc ggcatcgcgc agcgtggcct tatgggcctc ccattgctgc
     3301 agcagcgccg cgatgtgctc aagcgtgtga tattggcgcg tcggctcgcc gtaggcgcgc
     3361 atcagctcat cgcgcacggc ttcggcggcg gacgcgctcg caccctggcg cccggccagg
     3421 ctgcgccagg tcgccgagag caactcctca gcgctcatca ccattggctt cccactcact
     3481 ccagcttcag tccgaggttg cgtaccacct ctccccactt cttgatctcc gcccgcacgt
     3541 aggcctcgga ggtttcgggc gagccgccca cgggcagcag ccccaacggc tcgagccgtt
     3601 cgcggatcgg cgcctcggcc aacacggcat tggcgtcggc attgagcttg gcgagcacgg
     3661 ccggcggcgt tgccgcgggc acgaacagcg cgatccaggt ggattcctcg ctgtcgccat
     3721 agcccagttc gatggcggtc ggcacctgcg gcagggtcgg cacgcgctta tcgctgagca
     3781 ccgccaagcc tttgatctcc ccgctcttga tgagctcgat ggccggcggc atggcgaggc
     3841 aggcaagcat gatgtggccg cctaccagcg cgttgagaag gggcgcggcg cccgtgaacg
     3901 acaccggggg aatatcgacc ttggccagcg atttgaagat gcgctcggcc gacagatgcg
     3961 gcgtggtgcc gggtccaggc atgccatagg tgaatttctc gctccgcgcc gcctcgatca
     4021 gatccttgag cgtggaggcc ttcagcgttt tgctggcgac gagaatgttg ggcgtggtgg
     4081 ccgcgacgat ggccgtcttg aattcggtct cgggcgaata gcccgcgttg ctcgttaggc
     4141 tgggattaac ggcaaaggcg ctggtggtca cgagtacgga gtagccggtg gcctcggcct
     4201 tggcgatctg acgcgcggcg atgttgccgc cggccccggc gcgattttcg atgacgacgc
     4261 cttggcccca tttttcggcg agccgctggc cgaggatgcg cgccatggca tcggtcggcc
     4321 cgccggcggg aaaggcgacg acgaatttga cggtctggct cggataaacg tcttgcgcgg
     4381 ccgccggcac gccggcggcg aaagttgcgg cggcggccgc gagcacaagg cgacggcagc
     4441 cctgagcgag gctccttggc atagaggcgc cctccatcga cgttcctctc ggtccccgcg
     4501 tccgccgact ctaccagcaa aggcgctcgg ttgaagccgc gtccgttcgc tgctagatgt
     4561 tgtacgataa cctgacatcc atgtcacgag gtgcgctgaa ccgtgatgac gccagacctt
     4621 gcagggttgc agaaaacgct gggctcgggg ctcctcgcct ttcccgccac tgcctttgat
     4681 gccgcgctcg acttcgatga ggagacttac cgggagagca tcgcgctcaa tatcgggcat
     4741 ggcgccgtcg gtcttttcgc cccgggggga acgggggaat tcttttcgct gacgcccgag
     4801 gagtgccgtc gggtgacgcg tgcggccgtg gtcgcggcgg ctgggcgggt gcccatcgtg
     4861 gccggcaccg gctatggcac ggccttggcc gtcgagatcg ccaaagccgc cgaggcggcg
     4921 ggggctcaag cagttctggt gctgccgccc tatcttatca acgccgagcc gagcggactg
     4981 gaagcccacg tcgatgcgat ctgtcgcgcc gttggcattg gcgtcattct ctacaatcgc
     5041 gacaacagtc tttatgccgc cgagagcgtg gcgcggctgg ccgagcggca cgccaacctg
     5101 atcggtttca aggatggcca tggtgacgtc gaacagctgg tgcgcgtgcg ccgcctgctc
     5161 ggcaaccgct tgctctatat cgggggaatg ccgacggcgg aggtgtttgc cgtgccctat
     5221 ttcgcggccg gtttcaccac ttattcttcg gcggtctaca acttcattcc ggaaacggcg
     5281 caacgcttct atgccgccgt caaagccggc gacacgcgca cctgcgatga cttgctcagc
     5341 agctttttta taccctacat cgccctgcgc aatcgcaagc gcggctacgc cgtcgccatc
     5401 gtcaaggccg gcatgcggct gctggggcgg tcggccgggc cggtgcgccc gccgctgacg
     5461 gacctgaccc cagcggaaat ggacgaattc aaaaccctcc tcgagcgggc cttcggcggc
     5521 cgcgcccgct aagccaagcc gcgcgctccg tccaccgtgc cggcgcactg gcacggcggt
     5581 gcccggcgag cgcgccgcga ccccaaggaa acgcgaccgg cccggaggc
//
LOCUS       DASTMT010000398         4897 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_1859990, whole genome shotgun sequence.
ACCESSION   DASTMT010000398 DASTMT010000000
VERSION     DASTMT010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4897)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4897)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4897
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1859990"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..206)
                     /locus_tag="VFR00_09310"
     CDS             complement(<1..206)
                     /locus_tag="VFR00_09310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007800051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO1/SenC/PrrC family cytochrome c oxidase
                     assembly protein"
                     /protein_id="HEU0159502.1"
                     /translation="MRLTVVGVWLLAALGLLVGPAQAARWGKEYVPNVPVVTQDGKTL
                     NFYDDLIKDKIVVLSFIYTSCKDI"
     gene            complement(286..2658)
                     /locus_tag="VFR00_09315"
     CDS             complement(286..2658)
                     /locus_tag="VFR00_09315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020184861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HEU0159503.1"
                     /translation="MYISEKASRIRQREAQRARDNRAEIVKARSIGQITRRDLIRWGI
                     FTSGGLLVCKNGLSPLARSAFAEVPTGTPRSPLYGARKFNQPLNRLNYQTPTPLTLSA
                     LRGTETDVVFGGTDYGAELRPRRLSYHTDFSTPPVGTQRADFTNKLTNRGPLEGRPPG
                     EIFSHQRWLEFFPKEGYVFTLGSCAANQSFFGPGNGTAPFQGANAVWTYGRGNSRGAS
                     CGTLPPPLIKGRYGVPIMTRIYNYLPTDRKLNGGFGRNEHQLHFHNAHNGAESDGAAN
                     VHHFPGTFYDYRWSTTLARRDSINTDHSIRTTGGPDGNGGITPVAGDYREYQGTMWAH
                     DHRFFFTAENVYKGMLMMINYYSAKDRGREIPSGNKYSDDAKFGNLRLPSGKFLDHGN
                     LDFDVNLIISDAALDSDGQLFFDIFTTDGMIGDIPLVNFGFAPFMEVLPRKYRFRILA
                     ASMSRFWKFAIAGPTGSAVPFQFIANDGNLVVNPITLTELDEQGIAERYDIVVDFSNF
                     RVGDRLHLVNRLKQTSGNKPDGAVSLSQALQGDKNDPVLGALLQFRVVGSVPSVDMPG
                     QTLTMANSCGVNDTSQVPQVLTDQIPIVAPVRTRVVEFGRRGTGDSRNPATGQCTPDC
                     SEVVFNFPWTISVNGGQAHSMNANRISNVYPKAGDIEHWTYVNGGGGWDHPIHLHFEE
                     GITMNRGSGVIPATEKLVRKDVWRLRPSGQVQFQIQFGEYGGSYVNHCHNTVHEDFAL
                     LMRIQLLVGERTAVTDTPNPTEDGVTFTTPEILPEGDPAQRPALFVPKST"
     gene            3505..4509
                     /locus_tag="VFR00_09320"
     CDS             3505..4509
                     /locus_tag="VFR00_09320"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HEU0159504.1"
                     /translation="MPAHFAASLEHLARLTAGLRLDRLTRNDWHAALLLGGLGLLSAV
                     CAWLLWSWLGVLAALGCLAAIAAFAPRLPPEAIMRLYRAKPIDPAHGAQIIHTVEVLS
                     ARAGLAAAPAVYIIPSMTANAFTAGTPQRAGIAVTEGLLRRLSLRELTGVLGHEVGHI
                     ANNDLPIMSLADLMTRATQALSYLAIFLAIFNLPAFLLGDSDVSLLALLLLYLAPSIG
                     SLLQLSLARAREFDADLAGASLTGDPAGLAAALEKIERVQGTLWEDMMLPVPGRRIPA
                     PSVLRTHPATAERVARLEALEPAQLPAPIELPEEPMVSLAGLGPGEMRPRLRFPGVWF
                     "
     gene            complement(4521..>4897)
                     /locus_tag="VFR00_09325"
     CDS             complement(4521..>4897)
                     /locus_tag="VFR00_09325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0159505.1"
                     /translation="AAAFARARAAVGSGAGAELVSELQAALGAAGSHLYLGATEAAAA
                     ATVLFIRDGIGYCAPAALSPSTSARDVAPVRAAIADAAAAGVEWVCAEAELLSPRHRA
                     LVGLGLDVAFVRARWSLAPRRS"
BASE COUNT          815 a         1570 c         1605 g          907 t
ORIGIN      
        1 cagatgtcct tgcagctcgt gtagatgaag ctcaagacga cgatcttgtc cttgatgaga
       61 tcgtcgtaga agttgagcgt cttgccgtcc tgggtgacga cgggcacgtt gggtacgtac
      121 tccttgcccc agcgcgccgc ctgtgccgga cccaccagca gaccgagcgc tgctagcagc
      181 cacacgccca ccactgtcag ccgcatgccc tcctccagca aatggctgac caggggtatc
      241 ctcgcggaat acccccggtg catcttcaga tctgagaccc tatcctcaag tggacttggg
      301 cacgaatagg gccggccttt gggcgggatc accctcgggc aggatctcgg gcgtggtgaa
      361 tgtcaccccg tcctccgtcg ggttcggcgt gtccgtcacg gccgtccgtt cacccaccag
      421 caactggatg cgcatgagga gcgcaaagtc ctcgtgtacc gtgttgtggc agtggttgac
      481 gtaggagccg ccgtactcgc cgaactggat ctggaactgc acctgtcctg acgggcgcag
      541 gcgccagacg tccttgcgca ccagtttttc cgtggccggg atgaccccgc tgccgcggtt
      601 catggtgatg ccttcctcga agtggaggtg gatcggatga tcccagccgc cgccgccatt
      661 gacgtaggtc cagtgctcga tgtcgccagc cttgggatag acattcgaga tgcggttggc
      721 gttcatggaa tgggcttgac cgccgttgac gctgatggtc caggggaagt tgaacaccac
      781 ctccgagcag tcgggcgtgc attgaccggt cgccgggttg cgcgagtcgc ccgtgcccct
      841 gcgcccgaac tcgacgacgc gcgttctgac cggggccacg atcgggatct ggtcggtcaa
      901 cacctgtggc acctggctgg tgtcattgac gccgcaggag ttggccatgg tcagcgtttg
      961 tcccggcatg tcgacactgg gcaccgaccc caccacgcgg aactgcagga gagcaccgag
     1021 caccggatcg ttcttgtcgc cctgcagcgc ctgcgacagg gagacggccc cgtcgggctt
     1081 gttgccgctc gtttgcttca ggcggttgac gaggtgcaag cggtcgccga cgcggaagtt
     1141 ggagaagtcg acgacgatgt cgtagcgctc ggcaatgccc tgctcgtcga gctcggtcag
     1201 cgtgatgggg ttcaccacca gattgccgtc attggcgata aactggaacg gcacggccga
     1261 accggtgggt ccggcgatgg cgaacttcca gaaccgcgac atcgacgccg ccaggatgcg
     1321 gaagcggtat ttccgcggca atacctccat gaacggggca aagccgaagt tgaccagcgg
     1381 gatgtcgccg atcatgccgt cggtggtgaa gatgtcgaag aagagctggc cgtccgaatc
     1441 gagggcggca tccgagatga tgagattgac gtcgaagtcg aggttgccat ggtccaagaa
     1501 cttgccgctc ggcaggcgca ggttgccgaa cttggcatcg tcggagtact tgttgccgga
     1561 gggaatttcg cgcccacggt ccttggcgct gtagtagttg atcatcatca gcatgccttt
     1621 gtagacattc tcggccgtga agaagaagcg atgatcgtgg gcccacatgg tgccctggta
     1681 ctcgcggtag tcgccggcaa ccggggtgat gccgccgttg ccatcgggcc cacccgtggt
     1741 cctaattgag tggtccgtat tgatcgaatc gcggcgggca agcgtcgtgc tccagcggta
     1801 gtcgtagaag gtgccgggga agtgatgcac gttggctgca ccgtcacttt ccgcgccgtt
     1861 atgggcgtta tggaaatgca gttggtgctc gttgcgaccg aagccgccgt tcagcttgcg
     1921 gtcagtcggc agatagttgt agatgcgggt catgatcggc acgccgtagc ggcccttgat
     1981 caagggcgga ggcagggtgc cgcaggacgc gcctctggag ttgccccgcc cgtaggtcca
     2041 cacggcattg gcaccctgga agggggcggt gccgttgccg ggtccaaaga aactctggtt
     2101 ggcggcacag cttcccagcg taaagacgta accttccttg gggaaaaact cgagccagcg
     2161 ctgatgcgag aagatttcgc cgggcgggcg tccctccaag ggaccgcggt tcgtcagctt
     2221 attggtgaag tcagccctct gggtgccaac cggcggcgtg gagaaatcgg tatgataaga
     2281 tagccgccgc ggccggagct ctgcgccgta gtcggtcccg ccaaagacga catcggtctc
     2341 ggtgccgcgc agggccgaga gcgtgagtgg tgtgggagtc tgatagttca accggttcag
     2401 cggctgattg aattttctgg cgccgtaaag ggggctgcgc ggcgtgccgg tcggcacttc
     2461 ggcgaaggcg ctgcgcgcca aggggctcag cccgttcttg cacaccagca gcccacccga
     2521 ggtgaaaatc ccccagcgga tcagatcgcg ccgcgtgatt tgtccgatag aacgggcttt
     2581 gacgatctcc gcccggttgt cgcgcgcccg ctgggcttct ctttgccgga tgcgggaggc
     2641 tttttcagat atatacatgg tcgtttggcc cttaagtgga atgggaatat taccgggctg
     2701 gcaggctttg cgcggcgcgc cagcgcgcga ggcggcgttt ctatggcgcg cgcggctttt
     2761 gcgcgcgcat agagattgtg gaactggaaa tgaatccaag ctctttagac gcggagttcg
     2821 gttgcaagat gagcattgtg cggcaggccg gcctctggct tttgttgcgc gccgccagac
     2881 ctaaaaaggc ttgccgatcg taccggcatg atcgccgggc cgaaccggcc aggcgaccct
     2941 gcaggccatt gccacctcga tcggccttgg gctgggacgc gaccattgtc gacacccttg
     3001 agaatgtgag cgccggcgcg ctcgctgctc aaccgcctcc ccgcgtcaag ccgaactatc
     3061 cggacacggc acgcgcgtgc ctaggcacca aagtctcata ctcgaggtca ccagttcgcg
     3121 cgtaacagcg acccattgga attgggcggg agaacaaaga tagcccgcca atctattaat
     3181 actttagttc aatattacat tgccacgatt ttgcggcgct attagaattt tggccgggga
     3241 aacaggtgag gcagacccat ccgaaaggcc gattatttcc cctataggaa agctccgaga
     3301 ctgcgtccgg aagtcggagc gcggacactt cgcctcagcc gatttttatc aacacgcgcc
     3361 tctggcgccc gagcccgccg gctcgcgccg cacgggccgg ccgcaccgca ttcgcgtgag
     3421 cgctttgcgc tttgcctgcc gggccctatg ctgaagctcg tggcgttgcg gcctcaaagc
     3481 cctgcggcca gggggacacg ggtcatgccc gctcatttcg cggccagtct agagcatttg
     3541 gcgcgcctca ccgccggcct tcgcctcgat cgcctgacgc gcaatgactg gcacgccgcc
     3601 ctgctgctcg gcggccttgg cctgctcagc gccgtctgcg cctggttgtt atggtcctgg
     3661 cttggcgttt tggccgcgct aggctgtctt gccgccatcg ccgcgttcgc gccgcgcctg
     3721 ccgcccgagg ccatcatgcg cctttaccgg gccaagccga tcgatccggc ccatggcgcc
     3781 cagatcatcc acaccgtcga ggtcctgagc gcccgcgccg ggctggccgc ggcgcccgcc
     3841 gtctacatca tccccagcat gacggccaat gcctttaccg cgggcacacc gcaacgggcg
     3901 gggatcgctg tcaccgaagg cctgctgcgg cggctcagcc tcagggagct gacgggcgtg
     3961 ctcggccacg aggtgggcca catcgccaac aacgatctgc ccatcatgag ccttgccgat
     4021 ctgatgacgc gggccaccca ggcgctctcc taccttgcga tctttcttgc cattttcaac
     4081 ctaccggctt tcttgctcgg ggattccgac gtgtcgctgt tggcgctgct cctcctctat
     4141 ttggcgccca gcatcggcag cttgctgcag ctctcgctgg cgcgggcccg cgaattcgac
     4201 gccgatcttg ccggcgcctc gctgacgggc gaccccgcgg gcctcgccgc cgcgctcgag
     4261 aagatcgagc gcgtgcaggg tacgctttgg gaggacatga tgctccccgt gcccggacgc
     4321 cgcatacccg caccctcggt gctgcgcacc caccccgcca cggccgagcg cgttgcccgc
     4381 ctcgaggcgc tcgagccggc gcaactgcca gcgcccatcg agctgcccga ggagccgatg
     4441 gtgtcgcttg ccgggctggg acccggtgag atgcgcccgc gcttgcgatt tcccggggtt
     4501 tggttctagg cgcgccgcgc tcacgaccgg cgcggagcca ggctccagcg cgcgcgcacg
     4561 aacgccacgt cgagcccgag accgacaagg gcgcgatgcc gcgggctcaa cagctcggcc
     4621 tccgcgcaga cccactcgac gccggctgcg gccgcatcgg cgatcgccgc ccgcacgggg
     4681 gcaacgtcgc gggcggaggt cgacggggag agcgcggcgg gcgcgcagta gccgatgcca
     4741 tctcggatga aaaggaccgt ggcggccgcg gcagcctccg tggcgcccag atacaaatgc
     4801 gagcccgccg cgcccagcgc cgcctgcaat tcgctgacaa gctcggcccc ggcaccgctc
     4861 ccgacggcgg cgcgagcgcg ggcaaaggcc gccgccc
//
LOCUS       DASTMT010000399         4965 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_5363466, whole genome shotgun sequence.
ACCESSION   DASTMT010000399 DASTMT010000000
VERSION     DASTMT010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4965)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4965)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4965
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5363466"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..190
                     /locus_tag="VFR00_09330"
     CDS             <1..190
                     /locus_tag="VFR00_09330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018319646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEU0159506.1"
                     /translation="SSGLSAVPGNVGEYTWNGAYGTGFFADPAEKLVVVFGTAAPGEL
                     RKYYREQVQMLTYAAMTR"
     gene            388..1092
                     /locus_tag="VFR00_09335"
     CDS             388..1092
                     /locus_tag="VFR00_09335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006200746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HEU0159507.1"
                     /translation="MLDLSAFPITRRWPAQHPDRLQLYSLPTPNGVKVSIALEEIGLP
                     YEPHLVDIGKNQSWTPEFLSLNPNGKIPAILDPAGPEAKPLALFESGAILLYLTDKTA
                     KLMPGSAARRYETIQWVFFQMAFIGPMFGQVGYFYKFAGRDIADKRPLERYRDESKRL
                     LGVLEARLREGPWIMGEDYTIADISMLGWVRNLVGFYGARELVDFDALQHVPRWLERG
                     LARPAVQRGLTIPQRA"
     gene            1466..2269
                     /locus_tag="VFR00_09340"
     CDS             1466..2269
                     /locus_tag="VFR00_09340"
                     /inference="COORDINATES: protein motif:HMM:NF025033.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEU0159508.1"
                     /translation="MRVLARFYGPNGRISVVEEVGTGIRHYEEAGVSQSCVMAGGEAG
                     VDYIRLMAELLVGTENALLLGCGGGALASMLHRRGAKVTVVDVNPISFQLARTFFWMP
                     NAITCITADMRPFLRDETRSFQAIGIDVGGPRFSYDAVLDSASIAHVRRRLRPGGRVA
                     INISCEAPDDPVPARIADKFKAQGLEVWVFMENVPCPQELNAIILASDRREKSQALAK
                     VGGNGWVLARLSAPPVLAAAKPARARPKRARETAARRPGAPKLSRSMVG"
     gene            complement(2266..2523)
                     /locus_tag="VFR00_09345"
     CDS             complement(2266..2523)
                     /locus_tag="VFR00_09345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0159509.1"
                     /translation="MSLSRDVLVANAEELVDRAEQRIGLFKVHLHNRQRHGLECERLT
                     ARVGRMELELKRLQLYRAVMRTTHFADAVMPETLSVARLRS"
     gene            complement(2721..3617)
                     /locus_tag="VFR00_09350"
     CDS             complement(2721..3617)
                     /locus_tag="VFR00_09350"
                     /inference="COORDINATES: protein motif:HMM:NF045433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0159510.1"
                     /translation="MLIIVITVIGLLALAAAGFAQVTRGQVKAAASSVGSSTAQALAD
                     AGVQLAILDLVQARQDPAYQRRFAVGEKPFVCDAGNGRRLAVSVADEAGKVDINTADD
                     RLLQALLAGVGAPASQALADAIIDYRDDDNTRRPQGAEREQYSAAGRQGPKNALFSVV
                     EEIEQVLGVDADLAARLSPHITVYTGQAGLDATSASRELLLILARGLGQEPTPAQSDS
                     QEPTSEERNPLPGLRFMGAQGRRFFALHVEAFTEAGAFVRDAIVELGQNRTRPYTFLR
                     WHRGVRRDTDAGLPDSALSACP"
     gene            complement(3722..>4965)
                     /locus_tag="VFR00_09355"
     CDS             complement(3722..>4965)
                     /locus_tag="VFR00_09355"
                     /inference="COORDINATES: protein motif:HMM:NF012485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HEU0159511.1"
                     /translation="YGGQEQGRVASISRTTTATVSSGALPDAVSGQPAFLPGAPGIPS
                     ATAAPTTAIGGASPVTTPAPAPDLATPGAPPLAGRPPLAAAPLDDRSTGISVVADESN
                     NALIITASAQEYHRMRRILEQIDVQPNQVLLEATIAEVRLNDQLNMGVRWFLQTGNFQ
                     FRFTDLGATTATNSATNIATIVPPAVTPAFPGFSAFFNTPNAQVVINALSAITDVNIV
                     SSPTLMVLDNKRATLQVGDEVPIVTQSAVAVQVAGAPIVNSVNMRNTGIILNITPRIS
                     DGGRVVLDIEQEVSAAVTTTTSTITSPTIQQRRIKTTVSVDDGNCIVLGGLIQDSSTN
                     SRNQTPLIGEIPILGNLFKSKDDNIQRTELLIAITPKIVKDTGQIRAIAAEFRDKINL
                     STRPQRQAPPDRREQLDRVLR"
BASE COUNT          783 a         1644 c         1658 g          880 t
ORIGIN      
        1 atcttcgggc ctctcagcgg tgcccggcaa tgttggcgaa tacacctgga atggagccta
       61 cggcacgggc ttcttcgccg atcctgccga gaagctggtg gtcgtgttcg gtaccgcggc
      121 gcccggcgaa ttgcgcaaat attatcgcga gcaggtgcaa atgctgacct acgcggccat
      181 gacgcgctga ggcgccagcc caagtcgtcg ccactgcatc gtgactggaa ccggctgcaa
      241 ggctggcccc gtgccgtggc accccgccgt tggggcctgc cggcgtagtt tgccccgggg
      301 ggatcggcct tgacgctttc tccggcgctg cgcccgccct cgggtctcgc cgcggagttg
      361 agatgccctt tgctcgagag gagccccatg cttgaccttt ccgcctttcc cataacgcgc
      421 cgttggcctg cgcagcatcc cgatcgcctt cagctctatt cgctgccgac gcccaatggg
      481 gtcaaggtat cgatcgccct tgaggaaatc ggcctcccct acgagcccca cctcgtcgac
      541 atcggcaaaa accagtcctg gacccccgaa ttcctgtccc ttaatccgaa cggcaagatc
      601 cccgccatcc tcgatcccgc aggccccgag gctaagccgc tcgccctctt cgagtccggc
      661 gccattctcc tttaccttac ggacaagacc gcaaaactga tgccggggag cgcggcgcgc
      721 cgctacgaga cgatccagtg ggtgttcttc caaatggcct tcatcgggcc gatgttcggc
      781 caggtcggct acttttataa gtttgccggc cgcgacatcg ccgacaagcg cccgctcgag
      841 cgctatcgcg acgagtcgaa gcgtctgctt ggcgtgctcg aggcgcgcct gcgtgagggg
      901 ccatggatca tgggcgagga ctacaccatc gccgacatct ccatgctggg ttgggttcgc
      961 aacctcgttg gcttttacgg cgcccgcgag ctcgtagatt tcgatgcctt gcagcacgtt
     1021 ccgcgttggc tcgagcgcgg tctggcccgg cccgcggtcc agcgcgggct caccattccg
     1081 caacgggcct gagccgccga accggcgcac ggcgcaccgg ccgccggcgc agcggctggc
     1141 tttgcaacgc ttgtcgagac aacaaccggc ccagtgccag ccgcgcgcgt gaggcaacag
     1201 gcgcgggcaa ttttgcggtg gccgcatcga ggcgcgcgcg ttcgcgtgag cgttctacgg
     1261 ggcgtcgtcg acgcggttaa cgcgcgcgcc aacctgcggc cgcaggcgcg cgcgggcagc
     1321 acgatcgagc tcaccttgcc aaaggcgagt gttgcatcca ggcgatgcgg cctgcgaggt
     1381 gcgcggccct tgccgtcacg cgagtctgga cttcgactca cggctcttgc ataaaagctc
     1441 gatttgccga cggcacggaa acagcatgcg cgtcctcgct cgcttctacg gtcccaacgg
     1501 aagaatttcg gtcgtcgagg aggtgggcac cggcatccgg cactatgaag aggctggcgt
     1561 cagccaaagc tgcgtgatgg ccggcggcga agccggcgtc gactacatcc gcctgatggc
     1621 cgaattgctg gtcggcacgg agaatgcctt gctgttgggc tgcggcggag gcgcgctcgc
     1681 gagcatgttg catcgccgcg gcgccaaagt gacggtcgtc gacgtcaatc cgatcagctt
     1741 ccagttggcg cgcacctttt tctggatgcc caacgcgatc acctgcatca ccgccgacat
     1801 gcgccccttt cttcgtgacg agacgcgcag cttccaagcc atcggcatcg atgtcggtgg
     1861 cccgcgcttt tcctatgacg ctgtgctcga tagtgcgagc atcgctcacg tgcgccgccg
     1921 gttgcgcccg ggcggccgcg tggccatcaa tatctcctgc gaggctcccg atgaccccgt
     1981 gccggcgcgc atcgccgata agttcaaggc gcaggggctc gaggtctggg tgttcatgga
     2041 aaacgtgccg tgcccgcagg agctcaacgc catcatcctg gcctccgacc gcagagaaaa
     2101 gtcgcaggca ctggcaaagg tcggcggcaa cggctgggtg ctcgcccgct tgtccgcgcc
     2161 gcccgtgctc gccgcggcca agcccgcgcg ggcgcgcccc aagcgagcgc gcgagaccgc
     2221 tgcccgccgg ccaggtgcgc cgaagctatc gcgctcaatg gttggctaac tgcgcagccg
     2281 cgccacgctc agggtctcgg gcatgaccgc gtcggcgaag tgcgtggtgc gcatcaccgc
     2341 gcgatagagc tggaggcgtt tcaactccaa ctccatgcgg cccaccctgg cggtcagccg
     2401 ttcgcattcc agcccgtgcc tctgccgatt gtgcagatgc accttgaaaa ggccgatgcg
     2461 ctgctccgca cggtcgacga gctcttccgc gttcgcgacc aggacgtctc gactaagcga
     2521 catggtgcca ccccttgcag cacctcccgc tctctttcct cacgcggcca gggtgccatc
     2581 gaaccggggg gaggcaaaat cacgtcccgt cacgctttgt tgcagtcgtg agccggctcg
     2641 gggcccccag cccggcggcg gcccgttttc ggcgccctcc gcgggcgctt aagaagcttg
     2701 gccttgagcg acgtcttgat ctaggggcaa gcgctcaggg cggagtccgg caggccggcg
     2761 tcggtatcgc gccggacgcc gcggtgccaa cgcaggaagg tatagggtcg cgtccggttt
     2821 tggccgagct cgacgatggc atcgcgcaca aaggcgccag cctccgtgaa ggcctccacg
     2881 tgcagcgcaa aaaagcgccg gccctgcgcg cccatgaagc gcagccccgg gagcgggttg
     2941 cgctcctcgc tcgtcggctc ctgggagtcc gactgggcgg gcgtgggctc ctggcccagc
     3001 ccgcgcgcca gaattaagag cagctcgcgc gaggcactcg tcgcgtcgag gccggcctgg
     3061 cccgtgtaaa ccgtgatatg cgggctgagg cgggccgcca agtcggcgtc gacgccgagc
     3121 acctgctcga tttcctccac caccgagaag agcgcattct tcgggccctg gcggccggcg
     3181 gcgctatatt gctcgcgctc ggctccctgc ggccgtcggg tgttgtcatc gtcccgatag
     3241 tcgatgatgg catcggccag cgcctggctt gccggcgccc cgacgccggc cagcagggcc
     3301 tgcagcaggc gatcatcggc cgtattgatg tcgaccttgc cggcttcgtc ggcgaccgag
     3361 acggcgagac gccggccatt gccggcatcg caaacgaagg gtttttcgcc cacggcaaag
     3421 cgccgctggt aggccggatc ctggcgggct tgcaccaggt cgagaatggc aagctgcacg
     3481 ccggcgtccg ccagcgcctg cgccgtcgag ctgccgacgc tactagcggc ggctttgacc
     3541 tggccgcggg tgacctgggc gaagcccgcg gccgccaagg ccagcaagcc aatgacggtg
     3601 atgacgatga tcagcacaaa gccgccatcc tgcgctgcgg ccgcgcgagc gccgcaagct
     3661 ggcgcgctcc tgagcgttgt tggcaagctc ggcatacgca ttacctgagc gcgcgggccg
     3721 cctagcgcaa gaccctgtcg agctgttcgc gccgatcggg cggagcttgc cgctgcgggc
     3781 gcgtcgacaa gttgatctta tcgcgaaact cggcggcaat ggcgcgaatt tgtccggtgt
     3841 ccttgacgat cttgggcgta atggcaatca gcagctcggt acgttgaata ttgtcgtcct
     3901 tgctcttgaa caggttgccg aggatgggaa tctcgccaat cagcggcgtc tgattgcgcg
     3961 aattggtgga gctgtcctgg atcaggccgc cgagcacgat gcaattgcca tcgtcgacgg
     4021 agacggtggt cttgatccgc cgttgctgaa tggtcggcga ggtaattgtc gaggtggtgg
     4081 tcgtcaccgc cgcgctcacc tcttgctcga tgtcgagcac gacgcggccg ccgtcgctga
     4141 tgcgcggggt gatgttcaag atgatgccgg tattgcgcat gttgaccgaa ttgacgatcg
     4201 gcgcgcccgc cacctgcacc gccaccgccg actgggtgac gatcggcacc tcgtcgccca
     4261 cctgcagggt ggcccgtttg ttgtccagca ccatcagggt tggcgaggag acgatattga
     4321 cgtcggtaat agcgctcagc gcattaatca cgacctgcgc gttgggcgtg ttgaagaagg
     4381 ccgaaaatcc cggaaacgcc ggcgtgaccg cgggtgggac gatggtggcg atgttggtgg
     4441 cggaattggt cgcggtggtg gcgccgagat cggtgaacct gaactggaag ttccccgtct
     4501 gcaggaacca gcgcacgccc atgttgagct gatcgttcag cctcacctcg gcaatggtgg
     4561 cctcgagcag gacctggttc ggctgcacgt cgatttgctc caggatccgc cgcatgcggt
     4621 gatattcctg ggcgctggcg gtgatgatga gcgcgttgtt gctctcatcg gccaccacgg
     4681 atatccccgt ggagcgatca tccaggggcg cggcggcgag cggtggccga cccgcgaggg
     4741 gtggcgcgcc cggcgtcgcc aagtcgggcg ccggggccgg cgtcgtcaca ggcgacgccc
     4801 cgccgatcgc cgtcgtcggc gccgcggtcg cgctggggat gccgggagcc ccaggcagaa
     4861 atgcgggctg gcccgaaacg gcatcgggca acgctcccga cgacacggtc gccgtggtgg
     4921 tgcgggagat ggaggcgacc cggccttgtt cttgtccccc gtaga
//
LOCUS       DASTMT010000400         5733 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_3720163, whole genome shotgun sequence.
ACCESSION   DASTMT010000400 DASTMT010000000
VERSION     DASTMT010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 5733)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5733)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5733
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3720163"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(137..1729)
                     /locus_tag="VFR00_09360"
     CDS             complement(137..1729)
                     /locus_tag="VFR00_09360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU0159512.1"
                     /translation="MPQSHTQSPGPLERARGKDARDAVQEVVREIRARLLQGTVLRPE
                     FEYELLTMFVRNELSARVTVPLLAVIFSLASMFWAPVLQASAWLALVISAKFFMIAAC
                     RKFLAQPRSTVRVADWRRRIVLLELASGITWGGMAVVGFGTGDTASHVFMLTSLIVLL
                     AIRMTFASTLMTVLYVGTTPMTVAVVARLILQGHPFYYAMAAMAIGLHVYFLFLAKGL
                     NSTALSMLEFRAEKDALIAEIEEEKWISDEARQRAEAANVAKSRFLATMSHELRTPLN
                     AILGFSEVMKSELLGPIANANYKEYCANIHDSGRHLLQVINEILDLSRIEAGRYELQE
                     EAVRLCDVVEDCLRLLSLRAESKGLQVVPDCPRGLDQLWADERAVRQICLNLLSNALK
                     FTPRGGRITLSVCANGDGGQTLIVKDTGPGIPKEEIPRVLQAFGQGSLAHETAEGGTG
                     LGLPIVQNLIRLHGGTFELRSELRKGTEAIVSFPKGRVLKSMPPLQPLGQERHRQPPP
                     LLASVRRAGSEALRREGTTMLR"
     gene            1981..2592
                     /locus_tag="VFR00_09365"
     CDS             1981..2592
                     /locus_tag="VFR00_09365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007008174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HEU0159513.1"
                     /translation="MKIVQTKAAPNPRRVRIFLAEKGIEVPFEEVDLMQGALKTPEFT
                     ALNPFQRVPVLVLEDGTAIAETIAICRYFEALQPEPALFGKGPLASATVEMWSRRMDL
                     GLLLSVAQAFRHLHPAMAKLEVPQVAAWGEANKPRVLEILKIMDQQLAGRRFLAGEHY
                     SVADITGLVAVDFMKPARIERPAALENLARWYAEVASRPSAKA"
     gene            2637..3380
                     /locus_tag="VFR00_09370"
     CDS             2637..3380
                     /locus_tag="VFR00_09370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012253526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU0159514.1"
                     /translation="MKLTVIGCGDAFGSGGRLQTGYQVDWSGGRFLIDCGATVLIGLN
                     RCGLDPNAIERIFITHLHGDHFAGLVWWLIHAQHVGKRATPLTVIGPPGVGGRFASAA
                     EALFPGSSTLTRRYDLKFVELFTEAPLEAGDVRVTAFDAKHPSGAPSYALRFELEGKV
                     VAFTGDSEWVESLVPAGRDADLYIMECYEFEGAPRFHLSWSRIAAELDRIGAKRVLLT
                     HMGDTMLARRGEIVDGRVSFAEDGLTVEI"
     gene            3409..4038
                     /locus_tag="VFR00_09375"
     CDS             3409..4038
                     /locus_tag="VFR00_09375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013531711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HEU0159515.1"
                     /translation="MRVTARARALKRLVAEIGACRICRDSALGRPLPHEPRPVLRPSP
                     TARLCIASQAPGVRVHASGVPFSDASGDRLRAWLGLTAQEFYDQRRIAIVPMGFCFPG
                     HDAAGGDLPPRRECAATWHQRLFAAMPQLELILAIGSYAQRWHLGSPAAASMTAAVMA
                     WEAPLASPNRPRRLALPHPSWRNNAWLAQNSWFEQDLLPRLRQEVRRLL"
     gene            complement(4043..4546)
                     /locus_tag="VFR00_09380"
     CDS             complement(4043..4546)
                     /locus_tag="VFR00_09380"
                     /inference="COORDINATES: protein motif:HMM:NF016184.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur transferase domain-containing protein"
                     /protein_id="HEU0159516.1"
                     /translation="MHKIVKLNPRFAVTGALGPGDLTAAASSGFRAVLSNLPDGELPS
                     APVSALERELAQRAGLGFCHLPIPKGEIGWVRLAEQMLDALGQLPSPVLAHCASGQRS
                     ALAWAAAAAACHPVERVLAALSAAGFNFWPLSAELAGLAGLAGSRPIPAALDLPPPSH
                     TPRSDLS"
     gene            4684..5199
                     /locus_tag="VFR00_09385"
     CDS             4684..5199
                     /locus_tag="VFR00_09385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013947937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEU0159517.1"
                     /translation="MSLSKGSTPILDEMDVKILRILQQDCTRAVADIGKEVGLSTTPC
                     WRRIQKLEEAGVIKRRVAVLDPPRINAGVTVFVSIKTDQHSLAWLERFHTAVNDFPEV
                     IEFYRMSGEVDYLLRVVVPDIAAYDAFYKKLISRIEISKVSSAFAMEQIKYTTALPLD
                     FALRQGNGKEG"
     gene            complement(5305..5700)
                     /locus_tag="VFR00_09390"
     CDS             complement(5305..5700)
                     /locus_tag="VFR00_09390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007065560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEU0159518.1"
                     /translation="MQFLRPYRPQLLSVLRIATGLLILQHGSGKYLNFPVGPMNNASP
                     LTAGGAAGLIELAGGILITLGLFTRLAAFILSGTMAVAYFYAHAPRGFYPILNQGELA
                     VLYCFVFLYLAAAGAGPWSLDKALWHEAD"
BASE COUNT          995 a         1870 c         1909 g          959 t
ORIGIN      
        1 cggggtcgtt cgccggccgc cgtggttccg gcgaggggaa ggtgggtcgg agcggcggcg
       61 gcgcccagca tgctggcgat gaccctggct tgcttgggcg gcgtggccca cagcggatcg
      121 gcgaagcgca acgcctctag cgcagcattg tggttccttc gcgccgcagc gcctcgctgc
      181 cggcgcggcg cacactggcc agcaacggcg gcggctgccg gtggcgctct tggccgagcg
      241 gctgcagggg gggcatggat ttcagcaccc ggcctttggg gaaggagacg atggcctccg
      301 tccccttgcg cagttcggaa cggagctcga aggtaccgcc gtgcagtcgg atcaagttct
      361 gcacgatggg caggccgagg ccggtacctc cttcggccgt ctcgtgcgcc agcgagcctt
      421 gtccgaacgc ctgcagcacg cgggggattt cctctttggg gatgccgggc ccggtgtcct
      481 tgacgatcaa ggtctgaccg ccgtcgccgt tggcgcaaac gctcaaggtg atgcggcccc
      541 cacgcggcgt gaatttgagc gcgttggaca gaagattgag gcagatctgg cgcacggcgc
      601 gttcgtcggc ccagagctgg tcgagcccgc gcgggcagtc gggcacgacc tggagcccct
      661 tgctctcggc gcgcaagctc aacagacgca gacagtcctc caccacgtcg cacaggcgca
      721 cggcctcttc ctgcagctca tagcgcccgg cctcgatgcg ggacaaatct aagatctcgt
      781 tgatgacttg caggagatgg cggccgctgt cgtgaatgtt ggcgcagtac tccttgtagt
      841 tggcattggc gatcggcccc aacagctcgc tcttcatgac ttccgagaag ccaaggatgg
      901 cattgagcgg ggtgcgcagt tcgtggctca tggtggcgag aaagcgggat ttggccacat
      961 tcgccgcctc ggcccgctgc cgggcctcat cggagatcca tttctcctct tcgatttcgg
     1021 cgatcagggc atctttctcg gcccggaact ccagcatgga aagcgccgtc gaattgaggc
     1081 ccttggcgag aaacaagaaa tagacgtgga gcccgatcgc catggccgcc atggcgtaat
     1141 agaaggggtg cccctgcagg atcaagcggg ccacgaccgc aacggtcatc ggcgtggtcc
     1201 cgacgtagag cacggtcatc agcgtcgagg cgaaggtcat gcggatggcc aacagcacga
     1261 tcagcgaggt cagcataaag acgtgggacg ccgtatcccc ggtgccgaag ccgaccaccg
     1321 ccatgccacc ccaggtgatg ccgctcgcca gctcgagcaa gacgatgcgc cggcgccagt
     1381 cggcaacgcg caccgtgctg cgcggctggg ccaggaactt gcgacaggcg gcgatcatga
     1441 agaacttggc ggaaatcacc aacgccaacc aggcggaggc ctgcagcacc ggcgcccaga
     1501 acatggaggc gagcgagaag atgacggcca gcagcggtac ggtcacgcgc gcggagagct
     1561 cgttgcgcac gaacatggtc aggagttcgt attcgaactc gggcctaagc accgtgccct
     1621 gcagcagacg tgcccggatt tcgcgcacga cctcttgcac cgcgtcccgt gcgtccttgc
     1681 cgcgcgcacg ctcgaggggg cccgggctct gggtatgtga ttggggcacc gtctccctcg
     1741 ccatctcagc caatcaacaa acatgggaag cgacgcgctg gcgcatgcgc cgcgtcgatt
     1801 gacaatcgcc acaaaaccct aacgtcccgt ttatcaatgt attacggcgt tggcttgcgg
     1861 ccgcatttta gccacgtgag gcgcccaaac agcaaggcac acgctttgct tgcacgggca
     1921 actgcgctcc tctaggactg cggggagttg agcagcatgg ggaggccgat ggcgccagcc
     1981 atgaagattg tgcaaaccaa ggcggcgccc aatccgcgcc gggtgcgcat cttcctcgcg
     2041 gagaaaggca tcgaggtgcc cttcgaggaa gtcgacctca tgcagggcgc cctgaaaacg
     2101 cccgagttca cggcgctcaa cccctttcag cgcgtgcccg tgctggtgct cgaggatggc
     2161 accgccatcg ccgagaccat cgccatctgc cgctatttcg aggccctgca gccggagccg
     2221 gcactgttcg gcaagggtcc gctcgcctcc gcaaccgtgg aaatgtggtc gcggcgcatg
     2281 gacctcggct tgctcttgag cgttgcacag gcctttcgac acctgcatcc ggccatggcc
     2341 aagctggagg tcccgcaagt ggctgcctgg ggcgaagcca acaagccccg ggttctcgag
     2401 atcttgaaga ttatggacca gcagctggcc ggccggcgtt tccttgccgg cgagcattat
     2461 tcggtggccg acattaccgg cctggtggcg gtcgacttta tgaagcccgc gcgcatcgaa
     2521 cgcccagcgg cgctcgagaa cctcgccagg tggtatgcgg aggtggccag caggccgagt
     2581 gcgaaggcct gaagccacgg caagcagggt aggatcatgc agcccacagt cagatcatga
     2641 agctcacggt catcggctgc ggcgatgcct tcggctcggg tggccgcctg cagaccggct
     2701 accaggtcga ctggtccggc ggccgctttc tgatcgattg cggtgccacc gtgctgatcg
     2761 gcctcaaccg ttgcggcctc gacccgaatg cgatcgagcg gattttcatc acccacttgc
     2821 atggcgacca cttcgccggg ctggtttggt ggctgatcca tgcccagcac gtcggcaagc
     2881 gggccacgcc gctcacggtg atcggaccgc cgggcgtggg cgggcgcttt gccagcgccg
     2941 cggaggcgct gttccccggc tcgagcaccc tgacgcgccg ctatgatctg aaattcgtcg
     3001 agctcttcac cgaagccccg ctcgaagccg gagacgtgcg cgtcacggcc ttcgacgcca
     3061 agcatccctc gggcgcgcca tcctacgccc tgcgcttcga gctcgagggc aaggtggtcg
     3121 ccttcaccgg cgatagcgag tgggtggaaa gtctcgtccc cgccgggcgc gatgccgatc
     3181 tctacatcat ggagtgctac gaattcgagg gggcaccgcg ctttcatctg agctggagcc
     3241 gcattgccgc cgagcttgat cgcatcggcg ccaaacgcgt gctgctcacg catatgggcg
     3301 ataccatgtt ggcccgccgc ggcgagattg tggacgggcg cgtgtcattc gcagaagacg
     3361 gcctcaccgt ggagatatga ggcctccctg ccggcgcctc gggaggaaat gcgcgtgacg
     3421 gcccgcgccc gcgcgctgaa acgtctcgtt gccgagatcg gcgcctgccg catctgtcgc
     3481 gacagtgcgc tgggccggcc gctgccgcac gagccccggc ccgtgctgcg gcctagcccg
     3541 acggcgcgcc tgtgcatcgc cagccaggcc cccggcgtgc gcgtgcacgc ctcgggcgtg
     3601 cccttcagcg atgcctccgg agaccgcttg cgcgcctggc tcggcctgac ggcgcaagaa
     3661 ttctacgacc agcgccgcat cgccatcgtg ccgatgggct tctgctttcc cggccacgat
     3721 gccgccggcg gcgacctgcc gccgcggcgc gaatgcgcgg ccacctggca ccagcgtctg
     3781 tttgcggcca tgccgcaact cgagctgatc ctcgcgatcg gcagctatgc gcaacgctgg
     3841 caccttggct ccccggcggc tgccagcatg acggccgcgg tcatggcctg ggaagcgccg
     3901 ttggcaagtc ccaaccgacc gcgccggttg gccttgccgc atccctcctg gcgcaacaat
     3961 gcctggctcg cgcaaaattc ctggttcgag caggaccttc tgccccggct gcgccaggag
     4021 gtgcggcggc tgctctagag gactagctta ggtccgagcg cggggtgtga cttggcggcg
     4081 gcaaatcgag cgcggcagga attggcctgc tgccggcaag accggcgagg cccgccagct
     4141 cggcgctgag cggccagaag ttgaaaccag ccgcgctcag cgccgcgagc acgcgctcga
     4201 cggggtggca ggccgccgcc gcggccgccc atgccagcgc ggagcgctga cccgaggcgc
     4261 aatgggcgag cacgggggac ggcagctgcc cgagggcgtc gagcatctgc tcggccagcc
     4321 gcacccagcc aatctcgccc ttggggatcg gcagatggca aaagccaagg cccgcccgct
     4381 gggcgagctc gcgttccagc gcgctgacgg gggcactcgg cagctcgccg tcgggaaggt
     4441 tggagagcac cgcccggaag ccgctgctgg ccgccgccgt gaggtcgccg gggcccagcg
     4501 cgccggtgac ggcaaaccgc ggattgagct ttacaatttt gtgcatgcca cgccttcaga
     4561 ccctgggacg cccgagctct gaaaaaatag tgggtttttc gccgcattga cgcaatattg
     4621 aacctgcttt aggcacctat tgcgttacct ctagaaaaat gttccagcaa ggttatttac
     4681 cccatgtcgc tctcaaaggg gtccaccccg atacttgatg aaatggatgt caagatcctg
     4741 cgcatcctac agcaggactg tacgcgtgcg gttgccgata tcggcaagga ggttggcctg
     4801 tccacgacgc cgtgctggcg gcgcatccag aagctcgagg aggccggcgt catcaagcgg
     4861 cgggtggcgg tccttgaccc accgcgcatc aacgccggcg tcacggtgtt cgtgtccatc
     4921 aagacggatc agcattccct cgcctggctc gagcgcttcc acacggcggt caacgatttc
     4981 ccggaagtga tcgagttcta tcgcatgagc ggcgaagtgg actatctgtt gcgcgtcgtc
     5041 gtacccgata tcgccgccta cgacgccttc tacaagaagc tgatctcccg catcgagatc
     5101 tccaaggtct cctcggcctt cgccatggag cagatcaagt ataccacggc actgccgctc
     5161 gatttcgccc tgcgtcaggg caacggcaag gagggttgag gtcgtgagcg agatgcgcgc
     5221 ggcggccccg cgctgagccg gcttcggcgc ccgcgggcgg ggggcgccgg cggccctgcc
     5281 gcgcgggacg ttcttggatt ttcttcagtc ggcttcgtgc cacagcgctt tgtcgaggct
     5341 ccaaggccca gccccggccg ccgccagata aagaaagaca aaacaataaa gcacggccaa
     5401 ctcgccctga ttgaggatcg gataaaagcc gcgcggggcg tgggcgtaga agtaggcaac
     5461 cgccatggtg cccgacagga tgaaggctgc caggcgcgtg aaaagaccaa gcgtgatgag
     5521 gatcccgccc gcgagttcga tcagcccggc cgcgcccccg gcggtcaggg gcgaggcatt
     5581 gttcatgggg ccgacgggaa agttcaggta cttgccgctg ccgtgctgca ggatcaatag
     5641 ccccgtcgca atgcgcagga cgctcaggag ttgcggccga taggggcgaa gaaattgcat
     5701 ggcgcgcttc tccagccgtg gcgagagctc gaa
//
LOCUS       DASTMT010000401         1875 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_880492, whole genome shotgun sequence.
ACCESSION   DASTMT010000401 DASTMT010000000
VERSION     DASTMT010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 1875)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1875)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1875
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_880492"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..543
                     /locus_tag="VFR00_09395"
     CDS             <1..543
                     /locus_tag="VFR00_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002718458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0159519.1"
                     /translation="TRYRNEDIVMHQMIRLAIAAVLAIAFACPSAIATAQPVKQIKLT
                     DKHVEGFIAAQQDMAGVAEKMQGSTSDKPDPKIEAELESIAKKHGFQSFAEYDDVAGT
                     ISMVMAGIDPQTKAFTEPQIAIKKEIEEVSADKSIPEKEKKQMLDELNDALKSAAPIQ
                     FPSNIEIVTKYYDKIDAVLQ"
     gene            560..1399
                     /locus_tag="VFR00_09400"
     CDS             560..1399
                     /locus_tag="VFR00_09400"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008969720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEU0159520.1"
                     /translation="MPAPKSAARRVSPAGREAGELKRESAGAVRILTLARPEKRNSLS
                     EAMLGALQTAIEEADGEDAIRAVVLAAEGPVFSAGHDLKELTARRSDADGGRAYHAQI
                     MRQCSDMMLSIVRCRKPVIAAVQGPALAAGCQLVATCDLAVAATQATFATPGVNIGLF
                     CSTPMVALSRNVPRKRAMEMLLLGEQLGADKAADYGLVNRVVPAARVMAEALALARQV
                     AAKPKTTVAIGKEAFYRQLEMELADAYSYAAGVMVENMLHAEAKEGIGAFLEKRQPDW
                     EKS"
     gene            1463..>1875
                     /locus_tag="VFR00_09405"
     CDS             1463..>1875
                     /locus_tag="VFR00_09405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013419354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEU0159521.1"
                     /translation="MDHTAYSDTYIADILREARVIAMVGASGSNNRPSYFAMKYLLGK
                     GYRVLPINPTAMGQSILGQPVLGSLAELSPPVDIIDIFRASKAALDITREAIALKDKL
                     GLKVIWMQLGVRNEEAAAAAAAANLKVVMNRCPKI"
BASE COUNT          379 a          598 c          605 g          293 t
ORIGIN      
        1 acccgatatc gcaacgagga catagtcatg catcagatga ttcgcctggc gatcgctgcc
       61 gtcctggcca tcgctttcgc ctgcccatcc gccattgcga ccgcgcagcc cgtcaagcag
      121 attaagctga ccgacaagca cgtcgaaggc ttcatcgcgg cgcagcagga catggccggc
      181 gttgcggaaa agatgcaggg cagcacctcc gacaagcccg atcctaaaat cgaggccgag
      241 ctcgagtcga ttgccaaaaa gcacggcttt caatcgtttg ccgagtacga tgacgtcgcc
      301 ggcaccattt ccatggtgat ggccggcatc gatccgcaga ccaaggcgtt caccgagccg
      361 cagatcgcca tcaaaaagga aatcgaagag gtcagcgccg acaagtcgat ccccgagaaa
      421 gagaaaaagc agatgctcga cgaattgaat gatgccttga agtcggcggc gcccattcag
      481 tttccgagca acatcgagat cgtcaccaaa tattacgaca agatcgacgc ggtcttgcaa
      541 tagctcagga gcggtcagga tgccggcacc gaaatcggcg gctcgccgtg tttcgcccgc
      601 agggcgcgag gccggcgagc tcaagcgcga aagtgccggc gccgtgcgca ttctcacgct
      661 ggcccggccg gaaaagcgca acagcctgtc cgaagcgatg ctgggcgcgc tgcagacggc
      721 gattgaagag gccgacggcg aggatgccat ccgcgccgtg gtgcttgccg cggagggccc
      781 ggtgttttcc gctggccacg atttgaagga gttgaccgcc cggcgcagcg acgccgacgg
      841 cggccgtgcc taccacgccc agatcatgcg gcagtgctcg gacatgatgc tgtccatcgt
      901 gcgctgccgc aagccggtca tcgccgccgt gcagggaccg gcgttggctg cgggctgcca
      961 gctggtggcg acctgcgacc tggcggtggc ggcgacgcag gccacctttg ccacccccgg
     1021 cgtcaacatc ggcctgttct gctcgacgcc gatggtggcc ctgtcgcgca acgtgccacg
     1081 caaacgcgcc atggagatgc tgctgctggg tgaacagctc ggcgccgaca aggcggccga
     1141 ctatggcctc gtcaatcgcg tcgtgcccgc ggcgcgcgtc atggccgagg ccctggcgct
     1201 ggcccgacag gtcgccgcca agcccaagac caccgtggcg atcggcaagg aggccttcta
     1261 ccgccagctc gagatggagc tcgccgacgc ctacagctat gccgccggcg tgatggtgga
     1321 aaacatgctg cacgcggagg ccaaggaggg gatcggcgcc tttctggaaa agcgccaacc
     1381 cgactgggag aaaagctgac gttgcgttcg cggccggcgc ctgccacaag cggcggcgag
     1441 cagccgccat tgggccaggg ccatggacca caccgcctat agcgatacct acatcgccga
     1501 cattctgcgc gaggcgcgcg tcattgccat ggttggggcg agcggcagca ataaccgacc
     1561 aagttacttc gccatgaagt acctgttggg caaaggctat cgcgtgctgc caatcaatcc
     1621 gaccgcgatg ggccaaagca ttctcggcca accggtgctg ggttcgttgg ccgagctttc
     1681 gccacccgtc gatatcatcg acatcttccg cgcctcgaag gcggcgctcg acatcacgcg
     1741 cgaggcgatc gccctcaaag acaagctggg cctgaaggtg atctggatgc agctgggtgt
     1801 gcgcaacgag gaggcggcgg cggcggcggc ggcggccaac ctcaaggtgg tgatgaaccg
     1861 ctgcccaaaa atcga
//
LOCUS       DASTMT010000402         2491 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_1210455, whole genome shotgun sequence.
ACCESSION   DASTMT010000402 DASTMT010000000
VERSION     DASTMT010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2491)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2491)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2491
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1210455"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..628)
                     /locus_tag="VFR00_09410"
     CDS             complement(<1..628)
                     /locus_tag="VFR00_09410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PCRF domain-containing protein"
                     /protein_id="HEU0159522.1"
                     /translation="MTAIPTEKLDKLVERWQAIQGELARGANQSAYVALAKEFSELNP
                     VVQTIAELRKLQAQRDELAELLADPAADAELATIARDELATLQPRLDEVEQALRVQLL
                     PKDAADARSAILEVRAGTGGDEAALFAADLFRMYARYAEQRGWKVEIISLSENDLGGY
                     KEVVASIVGKDVFARLKYESGVHRVQRVPLTEASGRIHTSAATVAIMPE"
     gene            complement(713..1414)
                     /locus_tag="VFR00_09415"
     CDS             complement(713..1414)
                     /locus_tag="VFR00_09415"
                     /inference="COORDINATES: protein motif:HMM:NF024805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU0159523.1"
                     /translation="MKNGFVGERDVRLAGSTFPAFDASGELAAGRDEQIGKIFRNMRT
                     ALKVPRDALARRLATSPAVIDSFEAGAITALPHWKETERIVRSYCDLLRFDPEPVLWR
                     LRSQLQLAANYARAAPQPQLRTGAPRSGSRGEHSSAQPPGPRPGRRRRRARTLFVLST
                     PIVFAAGLVYLGPSASRPIYQAIALLPMPIAGPVRTGLDYVVGLTMPSRDGLTWIEVS
                     DPRSRKADKLPSRVQ"
     gene            complement(1511..>2491)
                     /locus_tag="VFR00_09420"
     CDS             complement(1511..>2491)
                     /locus_tag="VFR00_09420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative PEP-binding protein"
                     /protein_id="HEU0159524.1"
                     /translation="HRKYRALRDKPAVTKDGKRVGLYINAGLLVDMPHLAESGADGVG
                     LFRTELQFMISATLPRLEQQTQMYRAISTEAGGKPVVFRTLDVGGDKVLPYLRQAAEE
                     NPALGWRAIRLSLDRPGLLRTQVRALLRATAGAELRLLLPMVTAVGEIGMARALIDRE
                     LELLRRRASPLPTQVMLGVMIEVPALLFELDALLPRVDFVSVGSNDLLQYFFAADRNN
                     ARVAGRYDPLSSAPLRALRAIAEAARRHAKPLSLCGEMAGRPLEAMALLGLGYRAISM
                     APVSIGPIKSMVMSLDVSLLQPWLSRLIEAGEGNLRAQLKRFAEEHAVEI"
BASE COUNT          455 a          822 c          807 g          407 t
ORIGIN      
        1 cttctggcat aattgccacg gtagccgccg aggtatggat gcggccgctc gcctccgtaa
       61 gcggcacgcg ctgcaccctg tgcacacccg attcgtattt caagcgggcg aagacgtcct
      121 tgcccacaat ggaggcgacg acctccttgt agcccccaag atcgttctcc gacaggctga
      181 tgatctcgac cttccaaccc cgctgttcgg catagcgcgc gtacattcgg aagaggtcgg
      241 cggcgaacag ggccgcctca tcgccgcccg tgccggcgcg tacctccagg atggcgctcc
      301 tggcatcggc ggcatccttc ggcaggagtt gcacgcgcaa tgcctgttcg acttcgtcga
      361 gacgcggttg cagcgtggca agttcgtcgc gcgcaatggt ggcgagctcg gcatccgccg
      421 ccgggtcggc caagagttcg gcgagttcat cccgctgggc ttgaagcttg cgcaactcgg
      481 caatggtctg cacgacggga ttgagctcgg aaaactcctt tgccagcgcc acgtatgccg
      541 actggttggc gccccgggcg agctccccct gaatcgcttg ccaacgctca accagcttgt
      601 cgagcttttc ggtgggtatg gcggtcatgc gcgttggact cgacagcaat cggcaaggtg
      661 gaacgggaac ctctcgcccg caggggcgag ccggtaccgg tagaacggag cgctactgca
      721 cgcgcgaggg cagtttgtcg gccttgcgcg agcgcggatc actcacttcg atccaggtca
      781 ggccgtcgcg gctgggcatg gtcaagccca caacatagtc aaggccggtc cgcaccggtc
      841 ccgcgatcgg catcggcagc aacgcgatgg cttggtaaat gggccttgat gccgacggcc
      901 ccaagtaaac gagtccagca gcaaacacaa tcggcgtgct gagcacaaaa agcgttcggg
      961 cccggcggcg ccgccgcccc ggtcgggggc caggcggttg ggccgaggaa tgctcgcccc
     1021 tcgagcctga acgcggcgcg cccgtgcgca gttgaggctg cggcgcggcc cgcgcgtagt
     1081 tggccgcgag ctgcaactgg ctgcgcaagc gccacaggac gggttcgggg tcaaaacgca
     1141 gcagatcgca gtagctgcgc acaatccgct cggtttcctt ccagtgtggc aaggctgtga
     1201 tggcaccggc ctcgaagctg tcgatcaccg ccgggctggt ggccagtcgc cgggcgagcg
     1261 catcgcgcgg caccttcagc gccgtgcgca tgttgcgaaa gatcttgccg atctgctcat
     1321 cgcgacccgc ggccaattcg ccgctcgcgt cgaacgcggg gaatgtggac cccgccaggc
     1381 ggacatcgcg ctcaccaacg aagccgttct tcatacgcgt cactcaccgc accaccccga
     1441 cttggtcggc aatcttttgg ggaaagttgc cgatttgtca aagaggagaa gattacggca
     1501 ggtacatggc ctagatttcc acagcgtgct cctccgcgaa gcgcttgagc tgggcccgca
     1561 gattaccctc cccggcctcg atcagccgcg ataaccacgg ctgcagcaag gagacatcga
     1621 ggctcatgac catcgacttg ataggcccga tcgagacggg cgccatcgag atggcgcgat
     1681 agccgagccc gagcaaggcc atcgcttcca ggggtcggcc tgccatttct ccacacaaag
     1741 aaagcggctt ggcatggcgc cgggcggctt cggcgatggc gcgcagtgcc cgcaagggtg
     1801 ccgaggacaa gggatcgtat ctacccgcca cacgcgcatt gttgcgatcg gcggcaaaaa
     1861 agtactgcag cagatcgttg ctcccgaccg agacaaaatc gacgcgcggc agcagggcgt
     1921 ccagttcgaa cagcagcgcg ggcacttcga tcatgacgcc cagcatcacc tgcgtgggca
     1981 gcggcgaagc gcgccggcgt agcagctcga gttcacgatc gatcagggca cgcgccatgc
     2041 cgatttcacc caccgccgtc accatcggca gcagcaggcg cagctcggcg ccggcggtcg
     2101 ccctgagcaa ggcgcgcacc tgcgtgcgca gaagcccggg tcgatcgagc gacagacgaa
     2161 tggcgcgcca cccgagcgcg gggttttcct ccgcggcctg gcgcaggtag ggcagaacct
     2221 tgtcgccacc cacatccaag gtgcgaaaca cgacgggctt gccccccgcc tccgtcgaga
     2281 tcgcgcgata catctgcgtc tgctgctcga ggcgcggcaa ggtcgccgaa atcatgaact
     2341 gcaactcggt gcggaacagg ccgacgccgt cggcgcccga ctcggccagg tgcggcatgt
     2401 ccaccagcaa accggcgttg atgtagagcc cgacgcgttt gccgtccttg gttacggccg
     2461 gcttgtcgcg cagcgcccgg tatttgcgat g
//
LOCUS       DASTMT010000403         1827 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_882002, whole genome shotgun sequence.
ACCESSION   DASTMT010000403 DASTMT010000000
VERSION     DASTMT010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 1827)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1827)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1827
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_882002"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..275)
                     /locus_tag="VFR00_09425"
     CDS             complement(<1..275)
                     /locus_tag="VFR00_09425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HEU0159525.1"
                     /translation="MRSYDANNIFAKILRGELPAHKVYEDDETLAFMDIMPRCEGHTL
                     VVPKTPARNLLDANAAQLAACMKTVQKVSLAIVKAFNADGITLQQFN"
     gene            567..746
                     /locus_tag="VFR00_09430"
     CDS             567..746
                     /locus_tag="VFR00_09430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0159526.1"
                     /translation="MRSGALGRRERRSDTLIAAAVLALLVAGWVQGLSVARMEALWAL
                     RAMLVANAHASGAVR"
     gene            complement(729..>1827)
                     /locus_tag="VFR00_09435"
     CDS             complement(729..>1827)
                     /locus_tag="VFR00_09435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014494670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU0159527.1"
                     /translation="PATSNPFLAHAFLKALEDAGCVGGRSGWLPQHLTLKLGADDDIA
                     ACAPCYLKSHSQGEYVFDRGWAEAYERAGGRYYPKLQIAVPFTPVPGRRLLARPGPAQ
                     DANQLLLATAAVELVERNDLSGAHVTFLTEAEWFRLGAKGFLKRTDQQFHWRNAGYGS
                     FEDFLASLASRKRKAVRKERGEATVEGLEIRWLRGAEITEASWDALYAFYMQTGSRKW
                     GRPYLNRKFFSLLGAAMAEACLIVMVKRGSKPIAGALHLIGGDCLYGRYWGALEHHPF
                     LHFEVCYYQAIEFAIAHKLARVEAGAQGEHKLARGYLPTPTYSAHFIADPGLRRAVAD
                     YLKRERAYVDAAGKELAEYAPFRKGPLTNGT"
BASE COUNT          318 a          557 c          632 g          320 t
ORIGIN      
        1 tcgttgaact gctgcagcgt gatgccgtcg gcgttgaagg ccttcacgat cgccagcgag
       61 accttctgga cggtcttcat gcaggcggca agctgcgccg cattggcgtc gagcagattg
      121 cgggccggcg tcttgggcac cacgagggtg tggccctcgc agcgcggcat gatgtccatg
      181 aaggccaggg tttcgtcgtc ttcgtaaact ttgtgcgcgg gcagctcgcc gcgcaaaatc
      241 ttggcgaaaa tattgttggc atcatagctt cgcatgaaca gctcccgctt aagcgtcgat
      301 cccccggtga tctgtcgcag gcggcatctt gggcgtcaag cgtgggcgct tttgcaactc
      361 gccgttgcga gccccgcgcg caaaggccgg cacctggcgc cgttaagcca tggctaaccg
      421 cgagtcgaga ttgtcagcct tgcagtagag gaatgccaag gcctttgcgc gacagtgatg
      481 gcatcgctca catgggagcg caaattggca aacgaccgcg cctgggcttg gccacagggc
      541 ggcgggttga gggcgggtaa tgtgccatgc ggagtggcgc actagggcgt cgcgagcgac
      601 gctccgatac gctgattgca gcggcggtgc tggcgttact ggtggcgggc tgggtgcagg
      661 ggctcagcgt cgccaggatg gaggcgctct gggcgttgcg cgccatgctg gtggccaacg
      721 cgcatgcctc aggtgccgtt cgttaacgga cccttgcgga agggcgcata ttccgcgagc
      781 tctttgccgg cggcatcgac gtaagcgcgt tcgcgtttga ggtagtcggc gacggcgcga
      841 cgcaatcccg gatcggcaat gaaatgcgcc gaataggtgg gggttggcag atagccgcgc
      901 gcaagcttgt gctcgccttg cgcccccgcc tccacgcgcg cgagcttatg ggcgatggcg
      961 aactcgatcg cctgatagta gcagacctcg aaatgcagga agggatgatg ctcgagcgca
     1021 ccccaatagc ggccgtagag gcagtctccc ccgatcaggt gtaaggcccc ggcgatcggc
     1081 ttggagccgc gcttgaccat cacgatcagg caggcctcag ccatggccgc acccaacagc
     1141 gagaagaact tgcggttgag gtagggacgg ccccacttgc gcgagccggt ttgcatgtag
     1201 aaggcataaa gcgcgtccca gctcgcttcc gtgatctcgg cgcctctgag ccagcgaatt
     1261 tcgagaccct cgaccgtcgc ctcgccgcgc tccttgcgca ccgccttgcg cttgcgcgag
     1321 gccaacgagg cgaggaagtc ctcaaagctg ccgtagcccg cattgcgcca atgaaactgc
     1381 tggtcggtgc gcttcagaaa gcctttggcg ccgaggcgga accactcggc ctcggtgagg
     1441 aaggtcacat gcgcgcccga cagatcgttg cgctccacga gctcgacggc ggccgtcgcc
     1501 agcagcagct gattggcgtc ctgcgcgggc cccggccgcg ccagcaagcg acgaccgggc
     1561 accggcgtga agggaaccgc gatctgcagc ttggggtagt agcggccgcc ggcgcgctca
     1621 taggcctcgg cccagccgcg gtcgaacacg tactcgccct ggctgtgcga tttcaggtag
     1681 cagggggcgc acgcggcaat gtcgtcgtcg gccccgagtt tgagggtcag gtgctggggc
     1741 agccagccgc tgcggccacc gacacagcct gcatcctcca gcgccttcag gaaagcatgg
     1801 gcgagaaagg gattggaggt ggcaggt
//
LOCUS       DASTMT010000404         1650 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_1865218, whole genome shotgun sequence.
ACCESSION   DASTMT010000404 DASTMT010000000
VERSION     DASTMT010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 1650)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1650)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1650
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1865218"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..575)
                     /locus_tag="VFR00_09440"
     CDS             complement(<1..575)
                     /locus_tag="VFR00_09440"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEU0159528.1"
                     /translation="MQAPQSTRRLEADLGPGSRQWRTPAANEILGHHLPHLGPGDRLL
                     LRALTLCAARYVVAIEGLQHIRPAQDPLILVANHSTRREAVPVPAMLLLLRGGRRVHF
                     LADWNFRLIPGVGFLYARAEVVTVTRKSARPRFLNALKPLYAQALPTLERARAHLVAG
                     QCLGIFPEGEVNRDPFRLLRGRRGAARLSLET"
     gene            890..>1650
                     /locus_tag="VFR00_09445"
     CDS             890..>1650
                     /locus_tag="VFR00_09445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhN family protein"
                     /protein_id="HEU0159529.1"
                     /translation="MAGSLVLLAAALIALCGMLREIDLDQVLAAIRATPFNAVLGACG
                     LVLIGYGTLSFYDYFALRAVGRRDVPYAVAAYAGFTATAIGHGLGVGFFSGGAVRLRI
                     YSPFGLGVSEVAKIGFITGLTFWLGNLSALGLALAVAPEQAAAATKLPQWVNQALGVG
                     ALASVAAYIAWLRGGTRAIGVHNFRLLLANARLTFVQIGIGILELGVGCLATYLLLPA
                     TPSTDCLALAAAYVLAALFGFLCHAPASLGAFEVA"
BASE COUNT          251 a          521 c          554 g          324 t
ORIGIN      
        1 gtttccagcg acaaacgcgc cgccccgcgc cgtccgcgca gaagccggaa aggatcgcgg
       61 ttgacctcgc cttcgggaaa gatgccgagg cattggccgg cgaccaagtg cgccctggcg
      121 cgctccagcg tcggcagcgc ctgcgcgtaa agcggtttta aggcgttgag aaaacgaggc
      181 cttgccgatt tgcgcgtcac ggttacgacc tcggcacggg cgtagaggaa gcccacgccc
      241 gggatcaggc gaaagttcca gtcggcgagg aagtgcaccc tgcggccgcc tctcagcagc
      301 aacaacatgg ccggcaccgg cacggcttcc cgccgtgtgc tgtggttcgc gaccaggatt
      361 aagggatcct gtgccggacg aatatgctgc agtccctcga tggcgacgac gtagcgtgcc
      421 gcgcataggg tcagcgcgcg cagcagcagg cgatctcccg gaccaagatg cggaagatga
      481 tggccaagga tttcgtttgc cgccggggtt cgccattggc gcgagccggg gcctaggtct
      541 gcctcaaggc gccgcgtgga ctggggagct tgcatgccgc ggggctcatg caacgcgttg
      601 caatcgacgc gcgactataa cctcgccccc gatatgcccg agccgcgctg cagcacgatg
      661 aagctctgac gacacttggg tggcagcgag cgcggacagc aaaactcttc tgaggcacct
      721 ccctcaaggc ctccaggcct gcaatatcgg tgtcgcctcg gtttgccatt ctcgccaagc
      781 gaatcacgtc ctctggattt ggccgagctc gtcaccgatg cgcttgttgg cacgccttgc
      841 ttccctggcc cgcgacgcga tggcatgcgt cggctggaac agggttgcca tggccggcag
      901 tctggtgctg ctggcggctg ccttgatcgc gctgtgcggc atgttgcgcg aaatcgacct
      961 cgaccaggtt cttgccgcga tccgggcgac gccgttcaat gccgtgctcg gcgcctgcgg
     1021 ccttgtcctt atcggttatg gcacgctgag cttttatgac tacttcgcgc tgcgggccgt
     1081 cggccgccgc gacgtgccct atgctgttgc cgcctatgcg ggcttcaccg ccacggccat
     1141 cggacatggc ctgggtgtcg gtttcttcag cggtggggcg gtgcgcttgc gcatctattc
     1201 cccatttgga ttgggtgtca gcgaggtggc aaagatcggc tttatcaccg gcctgacctt
     1261 ctggctcggc aatctgagcg cgctcgggct ggccttggcg gtggcgccgg agcaggctgc
     1321 agcggcaacg aaactcccgc agtgggtcaa tcaggcgctg ggggtggggg cgcttgccag
     1381 cgtggctgca tacatcgcct ggctgagggg cggaacgcgc gccatcgggg ttcacaattt
     1441 ccgcctgctc ctggcgaacg cgcgattgac attcgtccag attggcatcg gcattctcga
     1501 gctcggggtc ggttgtctgg caacgtacct gctgttgccg gccacgccgt cgaccgattg
     1561 cctagccctt gcggcagcct acgtgctggc cgcgctgttt ggcttcttgt gccatgcccc
     1621 cgccagcttg ggggcgttcg aggtggcgat
//
LOCUS       DASTMT010000405         6467 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_1212336, whole genome shotgun sequence.
ACCESSION   DASTMT010000405 DASTMT010000000
VERSION     DASTMT010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 6467)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6467)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6467
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1212336"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..207)
                     /locus_tag="VFR00_09450"
     CDS             complement(<1..207)
                     /locus_tag="VFR00_09450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015934268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HEU0159530.1"
                     /translation="MSATSPLKSVCLDLPVSTEALRALEIGTVVYLNGRIYTAREGVY
                     KKAVEERAGLPAPAAALGSANFHCS"
     gene            complement(334..1431)
                     /locus_tag="VFR00_09455"
     CDS             complement(334..1431)
                     /locus_tag="VFR00_09455"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent alcohol dehydrogenase"
                     /protein_id="HEU0159531.1"
                     /translation="MRAAVLTEVNKPLQILDLEQEGPKAGEVRVAVKAAGVCMSDWHI
                     MNGDWPLPLPMVLGHEAAGIVVEVGPGVGNVKRDDHVIFSFRPHCGHCRYCSSGRTVL
                     CSGHNDTPRWRMHDGSARVKLNGEPVNQMARIGTFSEYVVCPAEQVVAVRKDLPWTHA
                     AIIGCSVATGVGAVIRHARVEPGSAVLVVGCGGVGLNVVQGAKLAGAKTIMACDLLDN
                     KLAYAREFGATHTINAKREDVRKRVAELTHGFGADYAFDAIGGEETAALVVDAIAPAG
                     HAILVGIPAFSARAAISPAAMVYGEKKLTGTYYGSIRPAVDFALLADLDLDKGIELER
                     LISRTYAFDEINEGFRLLLTGAVARGVITFP"
     gene            complement(1628..2590)
                     /locus_tag="VFR00_09460"
     CDS             complement(1628..2590)
                     /locus_tag="VFR00_09460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015934269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HEU0159532.1"
                     /translation="MIPIAHLQRTAEGLMAKAAIEIPEDYLGGLRRCADTEKGELSAF
                     VIKAMLDNYEAAKEDRRAMCGDTGVPRWYVKYGNEARVEGGPVALETALRRATAQATK
                     DVPLRPNRVHPLWRTDHDNNCGIGAPEIEYSFHPEGDWIDLTTVHKGGLFGSDYRMLF
                     PGDGIDGIKRFYLDTLIAFGRRGLACQPAIVGVGLGGSKDISMVLGKQAACLRSVGSR
                     NPDGKIAALEDELKELGNSIGMGAMGFMGSSMVVACHIEVGYCHTGGMPMSVHMFCLS
                     SRRATARVFPDGRAEFRSDPQWFTEYMRRATIDWSGPTQATAAE"
     gene            complement(2702..4429)
                     /locus_tag="VFR00_09465"
     CDS             complement(2702..4429)
                     /locus_tag="VFR00_09465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002716513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEU0159533.1"
                     /translation="MMLERLATDILILGSGGAGLFAALHAHQTNPGLAIAIASKGLLG
                     KCGCTRMVQGGYNVALHKGDSIERHFMDTIEGGKWLPNQKMAWTLVSKAIERVQELEN
                     EIGCFFDRNADGSLHGKAFAGQTFDRTVHKGDLTGIEIINRLMEQVWARPIERLEEHR
                     AVALIPAAGGQALAGVLLLDVRTGNFRFVSAKAVLLATGGGPTMYRYHTPSGDKSMDG
                     LAMALRLGLPLRDMEMVQFHPTGLLAGPDTRMTGTVLEEGLRGAGGHLLNGKGERFMQ
                     ARDPRAERATRDFVSRCIYEEMRAGRTSPHGGVYIKMAHLGPDQVAREFKGMVERCAD
                     CGFDLAAGLVEVVPTAHYLMGGVICDVDTSTELAGLFVAGEDAGGAHGANRLGGNGVA
                     NSTVFGGIAGESMARWIAAHPGHRAADEAVVHSELVRAQHPLGPPPGDLNRLRERLLD
                     CMWDEVGVIRNAAGLRRGGAALEALEQELLATGLADGERAFNLTWHDWLNLRSLIEVS
                     KAILAAAQQRENSRGAHFREDFPDAGDLATSSFTVVRQHGAGLSVSSEPVEFSIVRPG
                     ESLLPEAAQ"
     gene            complement(4442..4741)
                     /locus_tag="VFR00_09470"
     CDS             complement(4442..4741)
                     /locus_tag="VFR00_09470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009452736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b subunit"
                     /protein_id="HEU0159534.1"
                     /translation="MVHRLSGVALAAFLPLHFLALALALKGEVALDGFLRWTASPLVK
                     VAEVGLVFFLSVHVLGGIRILLVENLPWFQGQKQLVAGALLTAALLAFAFLLRAA"
     gene            complement(4816..5145)
                     /locus_tag="VFR00_09475"
     CDS             complement(4816..5145)
                     /locus_tag="VFR00_09475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009452735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase"
                     /protein_id="HEU0159535.1"
                     /translation="MNVRLYVLQRGSAALMLPLVLLHLAVIFYASSHDLSAADIFART
                     RGSAFWGTFYAVFVVAAAIHAAIGVRVIAAEWAGLRGAAPALVMWSFGLLLAALGLTA
                     VAAVVLA"
     gene            complement(5142..5858)
                     /locus_tag="VFR00_09480"
     CDS             complement(5142..5858)
                     /locus_tag="VFR00_09480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015934273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEU0159536.1"
                     /translation="MAEITVSLWRGKADGSYQLYRVPQRENQTVLDVVTWIQRHADPT
                     LAYRFACRVGMCGSCAMTVNGRPRWTCRTHTAKVVKNGKLEIGPLANLPIIKDLACDL
                     RPFFEKWQAAKGVFTPGLTRADAIERIIPHTAPRLAADAAIECINCAACYAACDTVRW
                     NPDYLGPAALNRAWTLVNDVRDTGQRQRLQAVAAAGGCHACHSHQSCQQYCPNALNPT
                     ASIAGLKRHTMTAYLKGELE"
     gene            complement(5864..>6467)
                     /locus_tag="VFR00_09485"
     CDS             complement(5864..>6467)
                     /locus_tag="VFR00_09485"
                     /inference="COORDINATES: protein motif:HMM:NF015369.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEU0159537.1"
                     /translation="RSLKRAAALSEDVILAWAMNGNPLPPQHGQPLRLIVPGWYGMGN
                     VKWLARIEALAEPYQGFQQAVGYHYRAAPGKPGTPVTHMRVKSLLVPPGIPDWYFRQR
                     LLDAGATELFGRAWSGGGVAIARVEVGINGRWEEATLAPQIDKFSWQGWRYLWHAAAG
                     DYELACRATDANGDTQPLEPRWDAGGFGNNAVHRVRVWVR"
BASE COUNT         1140 a         2160 c         2107 g         1060 t
ORIGIN      
        1 cgagcaatga aagtttgccg atccgagtgc ggctgccggg gccggcaggc cggcgcgctc
       61 ctcgacggcc ttcttgtaga caccctcgcg cgcggtatag atgcgaccgt tcaagtagac
      121 aaccgtgccg atctcgaggg cccgcagggc ctcggtgctg accggcaggt caaggcaaac
      181 gctcttgagg ggtgacgtgg cggacatgct cacctttttt ggcggacctc ggcatcctaa
      241 accgcgcgcg gccaaaagtg gatcccatgc tggttgttgg acctactttt tcggcgccgc
      301 cgccggtggg cgacaggcgc ggcgctcatg tgctcaggga aaggtgatga cgccgcgcgc
      361 cactgccccc gtcagcaaga gccgaaagcc ctcgttgatc tcgtcgaagg cgtaggtgcg
      421 gctgatcagg cgctcaagct cgatgccttt gtctagatcg aggtcggcca acagcgcgaa
      481 gtcgacggcg ggccgtatcg agccatagta ggtaccggtg agcttctttt cgccatagac
      541 catcgccgcc ggcgaaatgg cggcccgggc cgaaaacgcg gggatgccga cgaggatggc
      601 gtgaccggcc ggggcgatgg catccaccac caatgccgct gtctcctccc cgccgatggc
      661 atcgaatgca tagtcggcgc cgaagccgtg ggtgagttcg gcgacgcgtt tgcgcacgtc
      721 ctcgcgtttg gcgttgatgg tgtgggtggc accaaattcg cgcgcgtagg cgagcttgtt
      781 gtcgaggagg tcgcaggcca tgatggtctt ggccccggcg agctttgcgc cttgcacgac
      841 attgagcccg acgccgccgc agccgacgac cagtacggca gagcccggct ccacacgtgc
      901 atgccggatc acggcgccga cgcccgtggc gacgctgcag ccgatgatgg cggcatgcgt
      961 ccagggcagg tccttgcgca ccgcgacaac ctgttcggcc ggacagacga catattcgga
     1021 aaaggtgccg atgcgggcca tctgattgac gggctcgccg ttgagcttta cgcgcgcgct
     1081 gccgtcgtgc atgcgccagc gcggggtgtc attgtggccg ctgcagagca ccgtgcgccc
     1141 gctcgaacaa tagcggcaat gcccgcagtg cggccggaag gagaagatga cgtgatcgtc
     1201 ccgcttgacg ttgccgacac cgggaccgac ttccacgacg atgcccgcgg cctcatggcc
     1261 aagcaccatc ggcaacggta gcggccagtc gccgttcatg atgtgccaat cgctcatgca
     1321 caccccggcg gctttgacgg caacgcgcac ctctccggcc ttgggcccct cctgctctag
     1381 atccaagatt tgcaggggct tgttgacctc ggttagcacg gcagctcgca tggcacttct
     1441 cctcagcttg cggcgctagg aacggcaaag gcccgccagc atcttacctg ccagggcctg
     1501 ggcggaaagc cacgccgacg cggtcgcctg ggcaagcacg ccagggcctg cagacggcgg
     1561 ccgacaagga cggcgcggcc atgctgctcg ccggcgtgag gcctccccgc cggctcccat
     1621 gtggacctca ttcggccgca gtggcctgcg ttgggcccga ccagtcgatc gttgcgcgcc
     1681 gcatatactc cgtgaaccat tgcgggtcgc tgcggaactc ggcgcgccca tcggggaaaa
     1741 cgcgcgccgt cgcccggcgc gacgacaggc agaacatgtg cacgctcatc ggcatgccgc
     1801 cggtgtggca atagccgacc tcgatatggc acgccaccac catcgaggag cccatgaacc
     1861 ccatggcgcc catgccaata ctgttgccga gctccttgag ctcatcctcc agagcggcga
     1921 tcttgccgtc gggattgcgc gagccgacgc tgcgcaaaca ggccgcctgc ttgcccaaaa
     1981 ccatggagat atccttcgag ccgcctagac ctacccccac gatggcaggt tgacaggcaa
     2041 gcccgcggcg accgaaggcg atcagcgtat cgagataaaa gcgcttgatg ccgtcgatgc
     2101 cgtcgccggg aaagagcatg cgataatcgg aaccgaagag gccacctttg tgcacggtgg
     2161 taagatcgat ccagtcaccc tccgggtgaa aggagtactc gatctccggg gcgccgatac
     2221 cgcaattgtt gtcgtggtcg gtgcgccaga gcgggtgcac gcggttgggt cgcagcggca
     2281 catccttcgt cgcctgcgcg gtcgcgcgcc gcagcgccgt ctccagggcg accgggccac
     2341 cctccacacg cgcctcattg ccatatttga cgtaccagcg cggcacgccg gtgtccccac
     2401 acatggcgcg gcgatcttcc ttggcggcct cgtagttgtc caacatggct ttgataacga
     2461 acgcggagag ctcgcctttt tccgtgtcgg cgcagcgacg caaaccgccg agatagtcct
     2521 cggggatctc gatggcagcc ttggccatca agccctcggc ggttctttgc aagtgtgcaa
     2581 tcgggatcat cgccggacct ctcggatgag gaacgattat gtcccctccc caggcgcgcg
     2641 atgcaagggg aaagccgccc gatgcccgcg gcctgccgct ctcctgcagc gcctgcctgc
     2701 cttactgcgc tgcctccggc aacaaacttt cgcccggccg gacgatgctg aattcgacag
     2761 gctcggagct gacgctgagg ccggcgccgt gctggcgcac gacggtgaag cttgaggttg
     2821 caagatcgcc ggcgtcggga aaatcctcgc ggaagtgcgc gccccggctg ttctcgcgct
     2881 gctgcgctgc ggcgaggatg gccttggaca cttcgatcaa ggagcgcaag ttcagccaat
     2941 cgtgccaggt cagattgaag gcgcgctcgc cgtcggccaa ccccgtcgcc agcagctcct
     3001 gctccagggc ctcgagcgcg gcgccgccgc gccgcaagcc cgccgcattg cggatgacgc
     3061 cgacctcatc ccacatgcaa tcgagcaggc gctcgcgcag ccgattgagg tcgccgggcg
     3121 gcgggccaag gggatgctgg gcacgtacca gctcggagtg caccaccgcc tcatcagccg
     3181 cccggtggcc agggtgcgcc gcaatccagc gtgccatgct ttctccggcg atgccgccga
     3241 acacagtcga gttggcaaca ccgttgccgc ctaggcggtt ggccccgtgc gcgccgcccg
     3301 cgtcctcgcc cgccacgaac aggcccgcca gttcggtgga ggtgtcgacg tcgcaaatga
     3361 cgccgcccat caggtagtgc gcggtcggca caacttcgac caagccggcg gcgagatcaa
     3421 aaccgcagtc agcgcagcgc tcgaccatgc ccttgaactc ccgggcgacc tggtcgggac
     3481 caaggtgggc cattttgatg tagaccccgc catgcggact ggtgcgcccg gcccgcatct
     3541 cctcatagat gcagcgcgac acgaagtctc gcgttgctcg ctcggcgcgc gggtcgcgtg
     3601 cctgcataaa gcgttcgccc ttgccgttga ggaggtgtcc gccggcccca cgcaagcctt
     3661 cctccagcac ggttccggtc atgcgtgtgt cggggccagc cagcaagccg gtcggatgaa
     3721 actgcaccat ctccatgtcg cgcagtggca ggccgagccg caatgccatg gcgaggccat
     3781 ccatgctctt atcgccggac ggcgtatgat agcgatacat ggtcggccca ccgccggtcg
     3841 ccaggagcac ggccttggcg gacacgaagc gaaagtttcc cgtccgcaca tcgagcagca
     3901 acactccggc cagcgcctgc cccccggcgg ccgggatgag ggccaccgcg cgatgttctt
     3961 ccagacgctc gatcggccgc gcccacacct gctccatcag tcggttgatg atctcgatcc
     4021 ccgtgagatc gcctttgtgc accgtgcggt cgaaggtctg gccggcaaag gccttgccgt
     4081 gcaagctgcc atcagcgttg cggtcgaaga agcagccgat ctcgttttcg agttcctgca
     4141 cccgctcgat ggccttgctc accaatgtcc aagccatctt ctggttgggc agccacttgc
     4201 cgccctcgat ggtatccatg aagtggcgct cgatggagtc gcccttatgc agcgccacgt
     4261 tgtagccgcc ctgcaccatg cgcgtgcagc cgcatttgcc gagcaagcct ttgctggcga
     4321 tcgcaatcgc caagcccggg ttggtttggt gggcgtgcag tgccgcaaac agtccagcgc
     4381 cgcccgagcc gaggatcagg atgtcggtcg cgagcctctc gagcatcatg cccatcgtgc
     4441 ctcaggccgc gcgcaacaaa aacgcgaacg ccaacagcgc cgcggtcagc agcgccccgg
     4501 ccaccagctg cttttgcccc tggaaccaag ggagattttc gacgagcagg atgcggatgc
     4561 cgcccagcac atgcacgctc agaaagaaga cgagacccac ctcggcaact ttgacgagcg
     4621 ggctggcggt ccagcgcaaa aacccatcga gcgccacctc gcctttgagg gcgagcgcga
     4681 gcgccaaaaa gtgcaacggc agaaaggcgg ccaacgccac gcccgagaga cggtgtacca
     4741 aggcggcaag ccaaagggca tcgcggcgat ggcggccgct attgagggta tgccgctgcc
     4801 gatccccggg gcctatcatg ccagcaccac ggctgcgacg gcggtcaggc caagcgcggc
     4861 taacagcagg ccaaagctcc acatcaccag ggccggcgcc gccccgcgca atcccgccca
     4921 ttcggcggca atgacgcgca cgccgatcgc ggcgtggatg gcggcagcga caacgaagac
     4981 cgcgtaaaag gtaccccaaa aggcgctgcc gcgagtgcgg gcaaagatgt cggcggcgct
     5041 gaggtcatgg ctgctggcgt aaaagatgac ggcaagatgg agcagcacca gagggagcat
     5101 caaggcggcg ctgccacgct ggagcacata gagccgcacg ttcactcgag ctctcccttg
     5161 aggtaggccg tcatcgtgtg ccgcttcagc ccggcgatgg aggccgtagg gttcagcgcg
     5221 ttggggcagt actgctggca gctctgatgc gaatgacagg catggcaacc tcccgccgcc
     5281 gcaacggcct gcagccgctg ccgctggccc gtatcgcgca cgtcattgac aagcgtccag
     5341 gcacggttga gcgcggctgg gccgaggtag tcggggttcc agcgcaccgt atcgcacgcg
     5401 gcatagcacg cggcgcagtt gatgcactcg atggctgcat cggccgccag gcgcggcgca
     5461 gtgtggggaa tgatgcgctc gattgcatcg gcgcgcgtca accctggcgt gaacacgccc
     5521 ttggcggctt gccacttttc gaagaacggc cgcaggtcgc aggccagatc tttgatgatg
     5581 ggcaggttgg cgagcggccc gatctcgagc ttgccattct tcaccacttt ggcggtatgg
     5641 gtgcggcagg tccaacgcgg ccggccgttc acggtcatgg cgcagctgcc gcacattccg
     5701 acccgacagg caaaccgata ggccagcgtc gggtcggcgt ggcgctgtat ccaggtgacg
     5761 acgtcgagaa cggtctggtt ctcccgctgt ggcacgcggt agagctgata gctgccgtcc
     5821 gccttgccgc gccacagcga tacggtaatt tcggccattg ctgctatctt acccaaaccc
     5881 gaacgcgatg cacggcgttg ttgccgaacc cgcccgcatc ccagcggggt tccaggggct
     5941 gggtgtcgcc gttggcgtcc gtggcccggc aggccagctc gtagtcgccg gcggcggcgt
     6001 gccacaggta gcgccagccc tgccaagaaa atttgtcgat ttgcggcgcc agcgtggcct
     6061 cttcccagcg gccgttgatg ccaacctcga cccgggcgat ggcgacaccg ccgcccgacc
     6121 aggcacgacc aaagagctcc gtggcccctg cgtcgagcag ccgctggcgg aagtaccaat
     6181 cgggaatgcc gggcggtacc agcagcgatt tgacgcgcat gtgcgtgacc ggggtgccag
     6241 gtttacccgg cgcggcgcga taatgatagc cgacggcctg ctgaaagccc tggtagggct
     6301 cagcgagcgc ttcgatgcgc gcgagccact tgacattgcc catgccgtac cagccgggca
     6361 cgatcaaacg caagggctga ccgtgttgcg gcggcagcgg gttgccgttc atggcccagg
     6421 ccaagatgac gtcctcgctg agcgccgccg cgcgcttgag actgcgg
//
LOCUS       DASTMT010000406         1515 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_883557, whole genome shotgun sequence.
ACCESSION   DASTMT010000406 DASTMT010000000
VERSION     DASTMT010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 1515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1515
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_883557"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..627
                     /locus_tag="VFR00_09490"
     CDS             <1..627
                     /locus_tag="VFR00_09490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011501557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HEU0159538.1"
                     /translation="YSKVLHGEQYLTIHRPIPAAGSVIGRGRVKELLDKGREKGAVLY
                     VERQILDKADGQPIATMTSAAMLRGDGGFGGKPGPQPAPHALPDGPARLSLDLKVAAN
                     AALIYRLSGDRNPLHADPVAAAKGGFKMPILHGLCTYGIAGRAIVKSCCGDDPSRLRG
                     LQVRFSSPVFPGETIRTEMWPEGSRVAFRARVVERDVVVLNNGLATLA"
     gene            complement(653..1144)
                     /locus_tag="VFR00_09495"
     CDS             complement(653..1144)
                     /locus_tag="VFR00_09495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013168780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcB family DNA repair protein"
                     /protein_id="HEU0159539.1"
                     /translation="MKSLAAPWDVASDGRQAPLAREICRGVGRTLQAYGFAPLGEVTL
                     ANGRRADVIGLSAAAEIWIVEIKSSAEDFRTDQKWPEYRAFCDRFFFAVSPTFPRQVL
                     PADTGLLVADRYGGEIVRAAPEHRLAGARRRALTLQLARTAALRLNGVLDPEHVAPSL
                     SRA"
     gene            1298..>1515
                     /locus_tag="VFR00_09500"
     CDS             1298..>1515
                     /locus_tag="VFR00_09500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0159540.1"
                     /translation="MFFKRPQQDTSGAKRSGEVTTVAARPQPLQASDLRRMVDPGKLG
                     FKTTDELNPISGLIGQDRALKAIQFGTS"
BASE COUNT          244 a          516 c          511 g          244 t
ORIGIN      
        1 tacagcaagg tgctgcacgg cgagcagtat ctgaccatcc atcggccgat cccggcggcc
       61 ggcagcgtga tcggtcgggg gcgcgtcaag gagcttttgg acaaaggtcg ggagaagggc
      121 gccgtcctct acgtggaacg acagatcctc gacaaggccg acggccagcc gatcgccacc
      181 atgacctcgg cggcgatgct gcgcggcgat ggtggcttcg ggggcaagcc cgggccgcag
      241 ccggccccgc atgccctgcc cgacggccca gcgcgcttga gcctcgacct gaaggtggcc
      301 gccaacgccg ccttgattta tcggctatcc ggcgaccgca atcccctgca tgccgacccc
      361 gttgccgccg ccaagggtgg gttcaagatg ccgatcctgc acgggctatg cacctatggc
      421 atcgccggcc gcgccatcgt caaaagctgt tgtggcgatg atccctcgcg cctccggggc
      481 ctgcaggtgc gcttctcctc gccggtcttt cccggcgaga cgatccgcac ggagatgtgg
      541 cccgagggca gccgggtcgc tttccgggca cgcgtcgtcg agcgcgacgt cgtcgtgctc
      601 aataacggtc tggcgacgct ggcctgaacc cggcgcaatt tctgccgcag cctcaagcgc
      661 gagataggct cggcgccacg tgctcgggat cgagcacgcc gttgagccgc agcgccgccg
      721 ttcgcgccag ttgcagcgtc aaggcccggc gccgggcgcc ggcgagcctg tgctcgggcg
      781 ccgcgcgcac gatttcgccc ccatagcggt cggccacgag caggccggtg tcggcgggca
      841 acacctggcg cggaaaagtc gggctcacgg caaagaaaaa tcggtcgcag aaggcgcggt
      901 attcgggcca tttttgatcg gtgcggaagt cctcagcaga ggatttgatt tcgacgatcc
      961 aaatctcggc cgctgccgag aggccgatga cgtcggcgcg tcgcccgttg gccaaggtga
     1021 cctcgcccag gggcgcaaac ccgtaagcct gcaaggtgcg gcccacgcca cggcaaattt
     1081 cgcgtgccag cggggcttgc cggccgtcgc tggccacatc ccaaggcgcg gcaaggcttt
     1141 tcatctctgc caagctcgtt gctggccacc cgcctcgagg tccgccgcgc ggcccaagct
     1201 gcccgggggc gcttcgatcg aggacaccgg cgatcttttc ttgcggctca ccgccgccta
     1261 tggtgcggcc cggcgtcgtg tagcggtggg ttatcgcatg tttttcaagc gtccgcagca
     1321 ggacacatcg ggagccaaac ggagcggcga ggtgaccacc gtcgcggcgc ggccgcagcc
     1381 gttgcaggcc tcggacctgc ggcgcatggt cgaccctggt aagctcggct ttaagaccac
     1441 cgatgagctg aaccccatta gcggcctgat cggccaggat cgcgcgctca aggccatcca
     1501 gttcgggacc agcat
//
LOCUS       DASTMT010000407         1850 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_5366081, whole genome shotgun sequence.
ACCESSION   DASTMT010000407 DASTMT010000000
VERSION     DASTMT010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 1850)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1850)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1850
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5366081"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..336
                     /locus_tag="VFR00_09505"
     CDS             <1..336
                     /locus_tag="VFR00_09505"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018425944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEU0159541.1"
                     /translation="HAAHIFQTSRWSADRPVRLVMIGTDFEVRVWQALLKIPLGRAVT
                     YTDIARHLGQPSASRAVGSAVGRNPISFVVPCHRVLRGDGSLGGYHWGLTRKRALIGW
                     EMGRLAGRG"
     gene            728..>1850
                     /gene="egtB"
                     /locus_tag="VFR00_09510"
     CDS             728..>1850
                     /gene="egtB"
                     /locus_tag="VFR00_09510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018266426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ergothioneine biosynthesis protein EgtB"
                     /protein_id="HEU0159542.1"
                     /translation="MTSQSLAGDLASAASIESRLFRTRRLSLELAAPLSAEDMTVQPM
                     DDASPTKWHLAHVTWFFETFILSKHLPGYRTFDENFNYCFNSYYESQGARQPRPKRGL
                     LTRPSSERVFAYRAHVDEGLARLLAKGVAATSPIARLLEVGINHEQQHQELLLTDILA
                     LFAASPLRPAYAQLSSPPGPDAPEAMRFIEFAGGRSQIGHDGKGFAWDNELPRHDALI
                     HPFQLADRLVSNAEWLQFMADGGYRTASLWLSDGWGLGNREGWRAPLYWEEQDGEWFA
                     MTLHGLKPIDVAAPVTHVSYYEADAFARWAGKRLPTEFEWEFAAEGVPVAGNTLARRA
                     LRPLPAEGLPTDGTPRQMFGDVWEWTQSAYLPYPGYRPPA"
BASE COUNT          299 a          636 c          596 g          319 t
ORIGIN      
        1 cacgccgctc atatctttca aacctcgcgc tggtccgccg accggccggt ccggttggtc
       61 atgattggaa cggatttcga ggtccgggtc tggcaggcgc tcttgaaaat cccgctgggg
      121 cgcgcggtca cctacacgga cattgctcgc catttgggcc agcccagcgc ctctcgcgcc
      181 gtcggctcgg cggtcggacg caacccgatt tcgttcgtgg tgccttgcca tcgcgtgctg
      241 cgcggtgacg gcagcctcgg gggctatcat tggggtctca cccgcaagcg cgccttgatc
      301 ggctgggaga tgggtcgcct ggccgggcgc ggctagcggc gtcagcagtc gtcagcatcc
      361 tcgcgtgctc gctcgcttgt gagcgaacgt taccccatct cgcggctaag cttgccgtga
      421 tcggcggccg ccgcgttcgc gcggcggccg ccgtacgcca gcgcacaaac caccaccagg
      481 gcagcccatc acccgtgcca gaaccgttcc aaaccgcgcg agcgaggcaa agcgtgagaa
      541 gtttcggaac aaagaccgcg ccccgtgcat tcacgctcgc tgcccggtcg ggcaatccag
      601 gcatggcagg catgccggtg gcaacaaaac ggcgggagcg ggatggtctt gccttcgcct
      661 atgctgttga cggctgcccg acctggcgga gtgcgcccgg cggcaggcga cagaaacgag
      721 gaccttcatg acgtcgcaaa gcctcgccgg cgacctggcg tcggccgctt cgattgaaag
      781 ccgtttgttc cggacacggc gtctctccct cgaactggcc gcgccgctca gcgccgagga
      841 tatgaccgtg cagccgatgg acgatgccag tccgaccaag tggcacctgg cacacgtcac
      901 ctggtttttc gaaaccttca tcctttccaa gcacctgccg ggctatcgga cattcgacga
      961 gaacttcaac tattgcttca actcctatta cgagagccaa ggagcacgcc agccgcgtcc
     1021 gaaacgcggg ctcctgacgc ggccgtccag cgagcgcgtc ttcgcctatc gcgcccacgt
     1081 cgacgagggc ttggcgaggc tccttgccaa gggcgttgcg gcgacctcgc cgatcgcgcg
     1141 gttgctcgag gtgggaatca atcacgagca gcagcatcag gagttgttgc tgaccgacat
     1201 cttggcgctg ttcgcggcca gcccgttgcg ccccgcctat gcgcagctct catcgccgcc
     1261 aggccccgac gcccccgagg caatgcgctt catcgaattt gccggtggcc gcagccagat
     1321 cgggcatgat ggcaagggct ttgcctggga caacgagctg ccgcgccacg acgccttgat
     1381 ccaccccttc caattggcgg accgcctcgt cagcaatgcg gagtggttgc agttcatggc
     1441 cgacggcggc taccgcacgg cctcgctttg gctctccgac ggctggggtc tcggcaaccg
     1501 ggaaggctgg cgagcgccgc tctactggga ggagcaggat ggcgaatggt tcgccatgac
     1561 gctgcacggc ctcaagccca tcgacgtcgc cgcgcccgtg acgcacgtca gctactacga
     1621 ggccgatgcc tttgcgcgct gggccggcaa gcgtctgccc accgagttcg agtgggagtt
     1681 cgccgccgag ggcgtgccgg tcgccggcaa caccttggcg cgacgggcct tgcggccgct
     1741 ccccgcggaa ggcctgccaa cggacggcac gccgcgccaa atgttcggtg acgtgtggga
     1801 atggacccag agcgcctatc taccctatcc cggttaccgc ccgccggcgg
//
LOCUS       DASTMT010000408         2770 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_884762, whole genome shotgun sequence.
ACCESSION   DASTMT010000408 DASTMT010000000
VERSION     DASTMT010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2770)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2770)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2770
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_884762"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..297)
                     /gene="rpsB"
                     /locus_tag="VFR00_09515"
     CDS             complement(<1..297)
                     /gene="rpsB"
                     /locus_tag="VFR00_09515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006205162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEU0159543.1"
                     /translation="MALPAFNMRQLLEAGCHFGHQSHRWNPKMAPFIFGARNNIHIID
                     LAQSVPLLHQALLKVSDVVAHGGRVLFVGTKRQASDAIADAAKRSAQYYINHRWL"
     gene            complement(507..1688)
                     /locus_tag="VFR00_09520"
     CDS             complement(507..1688)
                     /locus_tag="VFR00_09520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011511925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEU0159544.1"
                     /translation="MARAPRRLSEVKRRALPAHCARLGLVLQGGGALGAYQAGVYQAL
                     AEHGYLPHWIAGISIGAINGAIIAGTPEKDRVVRLRTFWERLTESVPAKPLLAGDWAR
                     GVFNEWSALTTASAGVPGFFRPRLPPVWLQPWGTEAALSFYDTAPLRQTLEEFIDFDL
                     LNSGAVRLSIGAAHLLRGNSVYFDTFEEGFRLGPEHIMASAALPPGFPPVEIEGQYFW
                     DGGVLSNTPLEHLLDAERDQSMLVFQIDLFSARGAMPRNLFDVYERHKDILYSSRTRK
                     NTSDFRQAQELRQAVERLLEKLPASKKDDADVAEIVRKLKASETLKPSASMSVVHLIY
                     REKYYETQAKDYEFSRLSMEEHWQAGLADTRHTLKHEKRWLQPPRDLEAVRTFDLSQT
                     D"
     gene            2015..>2770
                     /gene="dusA"
                     /locus_tag="VFR00_09525"
     CDS             2015..>2770
                     /gene="dusA"
                     /locus_tag="VFR00_09525"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011663404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HEU0159545.1"
                     /translation="MIPFQPTRRFCVAPMMDWTDRHCRFFHRLLSSHARLYTEMVTAQ
                     AVLRGDRDRLLAFSPAEHPVALQLAGCEPTLLAEAAAVGEAYGYDEINLNVGCPSDRV
                     QEGRFGACLMAEPELVARCVAAMRRSLQVPVTVKCRIGIDDQDSERDLSRFIGIVADA
                     GCTSFIVHARKAWLKGLSPKQNRELPPLDYARVWALKASHPELEIIINGGIATLGSAQ
                     AQLAKVDGVAVGRSAYQNPYLLADVDRQLFGQKA"
BASE COUNT          449 a          910 c          920 g          491 t
ORIGIN      
        1 gagccagcgg tggttgatat agtactgggc cgagcgcttg gcggcgtcgg cgatggcatc
       61 ggacgcctgg cgcttggtgc cgacgaacag cacgcggccg ccgtgggcaa cgacgtctga
      121 gaccttgagc agcgcttggt gcaggagggg caccgactgg gccagatcga taatgtggat
      181 gttgttgcgg gcgccgaaga tgaacggcgc catcttgggg ttccagcggt ggctttgatg
      241 gccgaagtga cagccagctt ccagaagctg gcgcatattg aaggcaggca gcgccatggc
      301 gcagtctccg ttttccggtt tgacctccgc gcgccttgag agaaccgagc cccaaaaagt
      361 ccgaagttcg gtcaccggag cgtgcaggcg gggctttgca tccgccgcac gcgagaggcg
      421 cgcgtgtgtg atgggcgctg tgtagtggtt ttgcctgccc ctgacaaggg ccgcggccgc
      481 gcccggcccg cgcaagcacg ttccggttaa tccgtctggc tgagatcgaa ggtgcgcacg
      541 gcctccaggt ctcttggcgg ctgcagccag cgcttctcat gtttgagcgt gtgccgggta
      601 tcggcgagcc cggcctgcca gtgctcctcc atggagaggc gcgaaaactc atagtccttg
      661 gcctgggtct cgtaatactt ctcgcgatag atgaggtgaa cgacgctcat cgaggcgctg
      721 ggcttcaagg tttcgctcgc cttcagcttg cgcacgatct cggccacgtc ggcgtcgtcc
      781 ttcttgctgg cggggagttt ttccagcagc cgttcgaccg cctgtcgcag ctcctgcgct
      841 tggcgaaagt cgctggtgtt cttgcgcgtg cgcgatgagt agagaatgtc cttgtgcctt
      901 tcgtagacgt cgaacaggtt gcgcggcatg gcgccgcgcg cgctgaagag atcgatctgg
      961 aataccagca tgctctgatc gcgctcggca tcgagcagat gctcgagcgg cgtgttggag
     1021 agcacacccc cgtcccaaaa gtattggcct tcgatctcga ccggcggaaa gcccggcggc
     1081 agcgccgcgc tcgccatgat gtgctccggg ccgaggcgga agccctcctc gaaggtatcg
     1141 aaatagaccg agttgccccg caacaggtgc gcggcgccga tgctgagccg gacggcgccg
     1201 ctgttcaaga gatcgaaatc gatgaactct tcgagcgtct ggcgcagggg agccgtgtcg
     1261 taaaagctca gcgccgcctc cgtgccccag ggctgcagcc aaaccggcgg caggcgcggg
     1321 cgaaagaaac ccggcactcc cgcactcgcc gtggtgagcg cgctccattc gttgaagacg
     1381 ccccgcgccc agtccccggc cagcaagggc ttggcgggca cgctttcggt caggcgctcc
     1441 caaaaggttc tgagccggac aacgcggtcc ttctcgggcg tgccggcgat gatggcgccg
     1501 ttgatcgccc cgatcgagat gccggcgatc caatgcggca gatagccgtg ctcggcgagc
     1561 gcctgataga ccccggcctg ataggcgcca agcgcgcccc caccttgcag caccaggccc
     1621 agccgtgcgc aatgcgccgg caacgcccgg cgcttgacct cgctcaggcg ccggggggct
     1681 cgggccatgc ctgccgcggc cgcggcccgg cgcgaaatcc tggctcttct catcgggcct
     1741 ttaggggttg gttaagcctt taagctccgg cgacggcagc gagcttaccg tcgcgcccgt
     1801 taacgcttag ccctcacccc agggcgccag tcttggctgc agggaggccc aaggcaaggc
     1861 ttttcgggat cggccggagc cccgcgctgc cctcgcccgc gcggcggcga cggcgcggca
     1921 cgccgaggca aggcaatcgc tgttgcgcgc aacgccggct cgaggctaca ccccgcgcca
     1981 gcgaacgccc gcgaaagcac cctctttgag ttgcatgatc ccgtttcaac cgacacgccg
     2041 cttctgcgtc gcccccatga tggattggac ggaccggcat tgccgcttct tccaccgcct
     2101 cctgtccagc cacgcgcgtc tttatacgga gatggtgacg gcgcaggcgg tgctgcgcgg
     2161 ggaccgcgac cggctgttgg ccttctcgcc cgccgaacat cccgtggccc tgcagcttgc
     2221 cggttgcgag cccaccctgc tcgccgaagc cgcggccgtg ggcgaggcct acggctacga
     2281 cgagatcaat ctcaacgtcg gctgcccctc cgaccgcgtg caggagggcc gatttggcgc
     2341 ctgcttgatg gccgaacctg agctggtggc gcgctgcgtt gcagcgatgc ggcggtcgct
     2401 acaggtgccg gtcaccgtca agtgtcgcat cggcatcgac gatcaagaca gcgaacgcga
     2461 ccttagtcgc ttcattggga tcgttgccga tgcgggctgc acgagcttta tcgtgcacgc
     2521 gcgcaaggcc tggctgaaag gtctttcgcc caagcaaaac cgcgagctgc ccccgctcga
     2581 ctacgcgcgt gtgtgggcgc tcaaggcctc ccatcccgag ctggagatca tcatcaacgg
     2641 cggcatcgcc acgcttggct cggcgcaagc tcagctggca aaggtggacg gggtcgcggt
     2701 cggccgctca gcctatcaaa atccttacct cctcgccgac gtcgaccgcc aactgtttgg
     2761 ccagaaggcg
//
LOCUS       DASTMT010000409         3278 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_1538647, whole genome shotgun sequence.
ACCESSION   DASTMT010000409 DASTMT010000000
VERSION     DASTMT010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3278)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3278)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3278
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1538647"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..613)
                     /locus_tag="VFR00_09530"
     CDS             complement(<1..613)
                     /locus_tag="VFR00_09530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006612114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TCP-1/cpn60 chaperonin family protein"
                     /protein_id="HEU0159546.1"
                     /translation="MAAKDVKFAQDARERMLRGADILANAVRVTLGPKGRNVVLEKSY
                     GAPRVTKDGVTVAKEIELEDKFENMGAQMLREVAAKTSDVAGDGTTTATVLAQAIVRE
                     GAKSVAAGANPMDLKRGIDTAVSHVVEELKAKTKKVTSNDEIAQVGTISANGDREIGK
                     IIAEAMKKVGNEGVITVEEAKSLETELEVVEGMEFDRGYISPYF"
     gene            complement(819..1133)
                     /gene="groES"
                     /locus_tag="VFR00_09535"
     CDS             complement(819..1133)
                     /gene="groES"
                     /locus_tag="VFR00_09535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEU0159547.1"
                     /translation="MKFRPLHDRVVVRRVEEETKTAGGIIIPDTAKEKPMQGEILAVG
                     PGARDEAGKLVPLDVKKGDRVLFGKWSGTEVKIDGQDLLIMKESDILGIVEGKASVRA
                     AA"
     gene            1644..1913
                     /locus_tag="VFR00_09540"
     CDS             1644..1913
                     /locus_tag="VFR00_09540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016919791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="usg protein"
                     /protein_id="HEU0159548.1"
                     /translation="MPDRDFRAQLAGFSLTTAEILYRLPDYPLLLQSFVWQDYDVHPR
                     FPRLQHFLEFWARNLDGKLHSVKVAHKRLITPAEMRLVDGEFRLH"
     gene            complement(1926..>3278)
                     /locus_tag="VFR00_09545"
     CDS             complement(1926..>3278)
                     /locus_tag="VFR00_09545"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HEU0159549.1"
                     /translation="KHAALLLDVNSGNILYQSAAEQPRYPASLAKLMTLYLVFERLEQ
                     GKLDERSKLKISAHASAAAPSKLDLEPGEEISVADAVKALVTKSANDVAIALAEHIAG
                     SEERFARLMTEKATQIGMAATTFRNASGLPDPEQVTTARDMATLALRLMDDFPKRYAL
                     FATRTFTYKNETFRNHNTLLFHYQGTDGLKTGYTRASGFNLVASVRRGRKHVLGVVFG
                     GSTAAARNTTMRALLNIGLLKGANEVTRKRTLVAAGPPPSRAAATAAASRFDMAQVRP
                     VLVSRTEPDPGARGARPAEPTSIAALIERSAREPSPVADEAAPSPRAATASAAPALLA
                     PPRLVRGSPPSSLDEQALALRREAPAPAAATATNLGGMTIQVGAFPSADEAERRLAWV
                     RQRATAVLARHEGLTQPVKQGEKLFYRARFAGFDGAAAASACKELRALKVECLILRAD
                     "
BASE COUNT          488 a         1076 c         1104 g          610 t
ORIGIN      
        1 tgaagtaggg cgaaatgtag ccgcggtcga actccatgcc ctcgaccact tccagctcgg
       61 tctcgaggct cttggcctct tccaccgtga tcacgccctc gttgccgacc ttcttcatgg
      121 cctcggcgat gatcttgccg atctcgcggt cgccattggc cgagatcgtg ccgacctggg
      181 caatttcgtc gttggaggtg accttcttcg tcttggcctt gagctcctcc acgacgtgac
      241 tgacggcggt gtcgatgccg cgcttcagat ccatggggtt ggcgccggcg gcgaccgact
      301 tggcgccctc gcgtacgatc gcctgcgcca gcacggtggc cgtcgtcgtg ccatcgccgg
      361 cgacgtccga ggttttggcg gcaacctcgc gcagcatctg cgcgcccatg ttttcgaact
      421 tgtcctcgag ctcgatttcc ttggcgacgg tcacgccatc cttggtgacg cgaggagcgc
      481 cataggactt ctccagcacg acgttgcggc ctttcggacc gagcgtcacg cgcacggcat
      541 tggccaggat atcggcgccg cgcaacatgc gctcgcgggc gtcctgggcg aacttgacgt
      601 ctttagctgc catagtttgg cacctccatg ttgaagcgaa agggcaagcc ttctggttac
      661 ggttgcgcgg cggcgccccc cgagccgggg ctggcggcgg acccttgctg ctgtctttcg
      721 gcaccgccac gcgctgggag cgcgtcggtg ccagctgggc ccgtcagggc tcgccgcctg
      781 cgccagcggc aaatgaaagg tgaaagccaa agccgcgctc aggccgcggc gcgcaccgag
      841 gctttgcctt cgacgatgcc gagaatgtcg ctctccttca tgatgagcag atcctggccg
      901 tcgatcttga cttcggtgcc ggaccatttg ccgaacaaca cccgatcgcc tttcttgacg
      961 tcgagcggaa ccagcttgcc cgcctcatcc cgggctccgg gtccaacggc gagaatctcg
     1021 ccttgcatcg gcttttcctt ggccgtgtcc gggatgatga tgccacccgc ggtcttggtc
     1081 tcttcttcga cgcgacgcac gaccacgcga tcgtgcagag ggcgaaactt catggcctct
     1141 actccactct gtcagaacaa attttcctca gttttcggcg gtcgtccgtg ctgttggacc
     1201 acgcacccgc gccgacctca tcgcccgcat gggggtgccc gatcggcatc ggcgacggta
     1261 ataaaaagct ctgttagcag tgtcaagcgg agagtgctag ccccgcgcta gatgggccag
     1321 caggctgacc ccgcctgcgg ctgcccgcgc ggggcaccca gccacggtgc tttttgctcg
     1381 ggcccgcgcc gtggcggcgg ccgctccccc cacaatagcg ggaactgcgc cccggcgtgg
     1441 gccgttcctg agtgccgagc cgcggcgagg gcgccggggt cgccaggaaa tgccggggtc
     1501 gccaggaaaa gaggtgacaa tcccgggcgc tgcgccgggg cgcgtagaga tgagatgagt
     1561 tgtgggaact atgcaacctg tgaatcgaca aagtcggtta gcttcgggct ctgtgcggcg
     1621 tcacacaagg tcaggggaca cccatgcctg atcgcgactt tcgagctcag ctcgcgggct
     1681 tcagcctcac gaccgcggag atcctctatc gcctgcccga ctatcctttg ctgctgcaaa
     1741 gcttcgtctg gcaggactac gacgtgcatc cgcgctttcc gcgcttgcag catttcctgg
     1801 agttttgggc gcgcaacctc gatggcaagt tgcacagcgt caaggttgcg cacaaacgtc
     1861 tgatcacgcc ggcggaaatg cgcctggtcg acggcgagtt tcgcctgcac taggccaatc
     1921 ccgcgtcagt ccgcgcgcag gatcaggcat tcgaccttga gggcgcgcag ttccttgcac
     1981 gcgctggccg cggcggcccc atcgaagccg gcaaagcggg cccgataaaa gagcttctcg
     2041 ccctgcttca cgggttgcgt gaggccctcg tgccgcgcca gcacggccgt tgcccgctgg
     2101 cgcacccagg cgagccggcg ttcggcctca tcggcgctcg ggaaggcccc cacttggatc
     2161 gtcatgccgc cgaggttggt cgccgtcgcc gcggccggcg ccggtgcctc gcgccgcagg
     2221 gcgagggcct gttcatcgag gctggagggc gggctgcccc gcaccaggcg cgggggcgcc
     2281 agcaaggccg gtgcggcgct ggcggtcgcg gcgcgcgggg atggcgcggc ttcgtcggcg
     2341 acggggctcg gctcgcgcgc gctgcgctcg atcaatgccg caatgctggt cggctcggcc
     2401 gggcgcgcgc cgcgggcgcc gggatcgggc tcggtgcggc tgacgagcac cggcctgacc
     2461 tgggccatgt caaatctgga cgcggcagcc gtcgcggcgg cccgcgacgg gggaggcccg
     2521 gcggcaacca gcgtgcgctt gcgcgttact tcgttggccc ctttgaggag gccgatgttg
     2581 aggagcgcgc gcatcgtcgt gttgcgcgcg gcggccgtcg agcccccaaa cacgacgccc
     2641 agcacgtgct tgcgcccgcg ccgcaccgag gccaccaaat tgaagccgga ggcgcgcgta
     2701 tagccggtct tgagaccgtc ggtgccctga tagtggaaca acaaggtgtt gtggttgcgg
     2761 aaggtctcgt tcttgtaggt gaaggtccgg gtggcaaaca gcgcatagcg cttgggaaaa
     2821 tcgtccatca gccgcagcgc cagggtcgcc atgtcccgcg ccgtcgtgac ctgctccgga
     2881 tcgggcaggc ccgaggcatt ccggaaggtg gtggcagcca tgccgatttg ggtcgccttt
     2941 tccgtcatca aacgcgcaaa tcgctcctcg ctgccggcga tgtgctcggc gagcgcgatg
     3001 gcgacgtcgt tggccgactt ggtgacgagg gctttgaccg cgtcggcgac gctgatctcc
     3061 tcgccgggct ccaggtcgag cttggagggc gctgccgcgc tggcgtgcgc cgaaatcttg
     3121 agcttgctgc gctcgtcgag cttgccctgc tcgaggcgtt cgaacacgag ataaagcgtc
     3181 atcaatttgg ccagcgaggc cggatagcgc ggctgctcgg ccgcactttg ataaaggatg
     3241 ttgccactgt tgacgtcgag caggagcgcg gcgtgctt
//
LOCUS       DASTMT010000410         3269 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_4382792, whole genome shotgun sequence.
ACCESSION   DASTMT010000410 DASTMT010000000
VERSION     DASTMT010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3269
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4382792"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..365
                     /locus_tag="VFR00_09550"
     CDS             <1..365
                     /locus_tag="VFR00_09550"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0159550.1"
                     /translation="VVVSAAMTSLGGVLFAFFYNNLFPEQVFHISRSIELILAPIIGG
                     IGTLFGPIVGAFLLTSLAEGLGEVMRAIGVDVPGVKQIFYGLCLLVVVMFLPDGVWPP
                     LAKALRLDGGPSKDRSGC"
     gene            367..1092
                     /locus_tag="VFR00_09555"
     CDS             367..1092
                     /locus_tag="VFR00_09555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007594457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0159551.1"
                     /translation="MPALLEISGVSKSFSGLKAVEQASFDVPAGEIVGLIGPNGAGKT
                     TCFNLVAGVYRPDAGEIRLAGRRIDGWRPDQICRAGVGRTFQLVKPFAGLSVLENVVV
                     GALNQSPSVRAAKEIAVRVIEQLELKAKQHEPAGALTLPDRKRLEVARALATGPRILL
                     LDEVMAGLRPTETDRMVSLFKDLNARLGLTILMIEHVMRAVMALSRHVVVLHHGQVIA
                     AGTPAQVTRNPAVLECYLGEDQL"
     gene            1092..1799
                     /locus_tag="VFR00_09560"
     CDS             1092..1799
                     /locus_tag="VFR00_09560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014531226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0159552.1"
                     /translation="MLQVEQLDLFYGDAQALDGVCLEAEEGELVAIVGANGAGKSSLI
                     RSIAGMEKPRRGRIVFRGRDITGLASHLICNLGVGQVAEGRQIFPTLSVEENLEMGAL
                     LPRARGRMPQTLREIYRLFPRLDERRRQLAGTMSGGEQQMLAIARCLMGQPELIMFDE
                     PSLGLAPALVQDLFRTIRSISQRGLTVILVEQNVAASLKLASRAYVLENGRIVTSGTG
                     AALLSDARVRQAYLGLG"
     gene            complement(1800..>3269)
                     /locus_tag="VFR00_09565"
     CDS             complement(1800..>3269)
                     /locus_tag="VFR00_09565"
                     /inference="COORDINATES: protein motif:HMM:NF042682.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6537 domain-containing protein"
                     /protein_id="HEU0159553.1"
                     /translation="FSCLNGFCPSFVTVHGAKVRKADTKQVADSGIVNMLAGLPEPRP
                     PALDRPYTMLVTGVGGTGVVTLSAVLGQAAHLEGKGFGAIDMTGLAQKGGAVACHMRV
                     AREVEQIHAIRAGVAGADLVLGCDLVVTASNKVLETVKPDHTAIVYSDYEMTTADFTR
                     NANLKVPGAALRHAIEARAGKAPVHALDAHTLAVKLFGDSIAANMFLLGYAYQLGHVP
                     IGSRAIEQAIELNGAAVEMSRNAFRFGRLGAHDTAALARMLGPSALAKSKPQSLEDLI
                     ADRTAQLADYQDQALAERYRARIASIGDAERRLAPTLSGLTAAAARGYHKLLAYKDEY
                     EVARLFTGPAFEAALSEQFQARGKLSFHLAPPLLARRDKATGEPRKLEFGRWMLPVFR
                     LLAKGKRLRGTAWDPFGYTQERKLERQMIADYERLLDELSQRLSPQTHATATALASLP
                     LEIKGFGHIKLRNYKAAKVREAALLAQLRNPAAALKAAE"
BASE COUNT          533 a         1044 c         1100 g          592 t
ORIGIN      
        1 cggtggtggt gtcggcagcc atgacctcgc tggggggtgt gctgttcgcc ttcttctaca
       61 acaacctgtt ccccgagcag gtcttccaca tctcgcgctc catcgaactg atcctggcac
      121 ccatcatcgg cgggatcggt acgctgttcg ggcctatcgt cggcgccttt ctgttgacaa
      181 gcttggccga gggcctcggc gaggtcatgc gcgcgatcgg cgtcgacgtg cccggcgtga
      241 agcaaatttt ttacggtttg tgcctgctgg tcgtggtgat gtttctgccc gacggcgtct
      301 ggccgccgtt ggccaaggcg ctgcggctcg acggcgggcc aagcaaggat cgctcggggt
      361 gctagcgtgc cggccttgct cgaaatcagc ggcgtgtcga agagcttcag cggcctcaaa
      421 gccgtcgagc aggcgagctt cgacgtcccc gcaggcgaga ttgtcggcct gatcggtccc
      481 aacggcgccg gcaagaccac ctgcttcaac ctggttgccg gtgtctatcg tccggacgcg
      541 ggcgaaatcc gcttggcggg acggcgcatc gatggctggc gtccagacca gatctgccgg
      601 gcgggggtcg gacgcacgtt tcagctggtg aagccctttg cgggcttgag cgtgctggaa
      661 aacgtcgttg ttggcgcgct caaccaaagt ccaagcgtgc gcgcggccaa ggagattgca
      721 gtccgcgtaa tcgagcaact ggaactcaag gccaagcaac acgagccggc cggcgccttg
      781 acgttgccgg accgcaagcg cctggaggta gcccgcgcgc tggcgacggg cccccggatt
      841 ctgttgctcg atgaagtcat ggccggcctg cgtcccaccg aaacggatcg gatggtttcg
      901 ctgttcaagg atctcaacgc ccggctcggc ctgacgatcc tgatgatcga gcatgtcatg
      961 cgcgccgtga tggcgctgtc gcgccatgtc gtggtcctgc accatggaca ggtcatcgcc
     1021 gccggcacac ccgcgcaggt gacgcgcaat cccgccgtgc tcgagtgcta tctcggcgag
     1081 gaccaactgt gatactccag gtagagcaac tcgacctgtt ttatggcgac gctcaggcgc
     1141 tcgatggggt ttgcctcgag gccgaggagg gcgagctggt cgccatcgtc ggcgccaacg
     1201 gtgcgggcaa gtcgagcctc atccgcagta tcgccggcat ggaaaagccc cgccgcgggc
     1261 gcattgtttt tcgcggccgc gacatcaccg ggctggcgag ccacctcatc tgcaatctcg
     1321 gcgttggcca ggtggccgag ggccggcaga tttttccgac gctcagcgtt gaggaaaatc
     1381 tcgaaatggg cgcattgttg ccgcgcgcac gcgggcgcat gccgcagacc ctgcgcgaaa
     1441 tctatcgcct ctttccccgc ctcgacgaac ggcgcagaca gctggccgga accatgtcgg
     1501 gaggcgagca gcagatgctg gcgatcgcgc gctgcttgat gggccagccc gagctcatca
     1561 tgttcgacga gccgtcgctt ggcctggcac ccgcgctggt ccaggatcta tttcgcacca
     1621 tccgcagcat cagccagcgc gggctgacgg tgatactggt cgaacagaac gtcgccgcct
     1681 cgctgaaatt ggcgagccgc gcctacgtgc tcgagaacgg acgcatcgtc accagcggca
     1741 ccggtgccgc cttgctgagc gacgcccgcg tacgccaggc ttatctcgga ctaggctagc
     1801 tattcagcgg ctttcaaggc tgccgccggg ttgcgcagct gggcgagcaa tgccgcctcg
     1861 cggaccttgg ccgccttgta attgcgcagc ttgatgtggc caaaaccctt gatctcgagg
     1921 ggcagggaag cgagcgcggt ggcggtggcg tgggtttgcg gcgacagacg ctggctcagc
     1981 tcatcgagca gccgctcgta gtcggcaatc atctgccgct cgagcttgcg ttcctgtgta
     2041 tagccgaagg gatcccaggc cgttccgcgc agccgcttgc ccttcgccag caggcgaaac
     2101 accggcagca tccagcggcc gaactcaagt ttgcgcggct cgccggtggc cttgtcgcgg
     2161 cgtgccagca gcggcggcgc aagatggaag ctgagcttgc cgcgcgcctg aaattgctca
     2221 gacaaggccg cttcgaaggc agggccggtg aacagtcgcg ccacttcgta ttcgtccttg
     2281 taggcaagca gcttgtggta gccgcgcgcg gccgcggcgg tgaggccgct gagagtgggc
     2341 gccaggcggc gctcggcgtc gccgatgctg gcgatccgcg cgcgatagcg ctccgccaga
     2401 gcctgatcct ggtagtcggc gagctgagcg gtgcgatcgg cgatcaggtc ctcgagcgac
     2461 tgcggcttgg atttggcgag cgcagatggg ccgagcatcc ttgccagtgc cgccgtatcg
     2521 tgcgctccca acctgccgaa gcgaaaggcg ttccggctca tttcgaccgc ggcaccgttg
     2581 agctcgatgg cctgctcgat cgccctggag ccgatcggca cgtgcccaag ctggtaggcg
     2641 tagccgagca gaaacatgtt ggcggcaatc gagtcgccaa acagcttcac ggccagggtg
     2701 tgtgcatcca gcgcatgcac cggcgccttg ccggcccgcg cctcaatggc atggcgcagc
     2761 gccgcgccgg gaactttcaa attggcgttg cgggtgaagt cggccgtcgt catttcgtag
     2821 tccgagtaga cgatggccgt gtggtcgggt ttgacggtct cgagcacctt gttggaggcc
     2881 gtgacgacga gatcgcaacc gagcaccaga tcggcgccgg ccaccccggc gcggatggcg
     2941 tggatctgct ctacctcgcg ggccacccgc atgtggcaag caacggcgcc gcctttttgc
     3001 gccaagcccg tcatatcgat ggcgccgaag cccttgcctt ccaggtgtgc cgcctgcccc
     3061 agcacggccg acagcgtcac caccccggta ccgccgacgc cggtcacgag catggtatag
     3121 ggacgatcga gggcgggcgg gcgcggctcc ggcaaacccg ccagcatgtt gacgatgccg
     3181 ctatcggcca cctgttttgt gtcggccttg cgcaccttgg cgccgtgcac ggtaacgaag
     3241 ctcgggcaga acccgttgag acaggagaa
//
LOCUS       DASTMT010000411         3749 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_2850943, whole genome shotgun sequence.
ACCESSION   DASTMT010000411 DASTMT010000000
VERSION     DASTMT010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3749)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3749)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3749
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2850943"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..538
                     /locus_tag="VFR00_09570"
     CDS             <1..538
                     /locus_tag="VFR00_09570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0159554.1"
                     /translation="RVKAHKDDRAEINASAQTEAKGLTAKRASDLLADCLKPLATHVA
                     KSQGPAGASDQASAKAWEAIDEVVATALTATNGALSQAGARLVPLDAPAWHADAQGHR
                     LPLAIEVYGKDVARMHIDHLGAEVEVSVGVPDALFINLGRRRRIPVAGLATHPLAEVI
                     ADCAWPVISRSRDSRRSA"
     gene            complement(611..973)
                     /locus_tag="VFR00_09575"
     CDS             complement(611..973)
                     /locus_tag="VFR00_09575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020181015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU0159555.1"
                     /translation="MVRILLAEDDAATRDLVQRALGLDGHEVVVTQDGSEALEYLRAA
                     QAPPDMLISDVQMPGLDGIALVEASLKVAPGLRVLLMSGFADELGRAEHLRSKIARII
                     SKPFTLEQIRTAVKGALG"
     gene            complement(997..1545)
                     /locus_tag="VFR00_09580"
     CDS             complement(997..1545)
                     /locus_tag="VFR00_09580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020083952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HEU0159556.1"
                     /translation="MDNGVPAHIETIFSATAIAERIEALAADIAGAGLQDLLAVAVLK
                     GSFVFAADLIRALYRVGLSPEVDFISLSSYRAGTRSLGRIEILRDIETDVHGRNVLLI
                     DDILESGRTLAFAKDLIMARGAARVLSCVLLDKPVARAANITADIRAFECPNVFVVGY
                     GMDLAHRFRELPFVGRLTRQSP"
     gene            1717..2373
                     /gene="ftsE"
                     /locus_tag="VFR00_09585"
     CDS             1717..2373
                     /gene="ftsE"
                     /locus_tag="VFR00_09585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEU0159557.1"
                     /translation="MIRLNGIGVNYEGGNHVLRDASFHLRPGSFHFLTGPSGSGKTTL
                     LRLLFMSMQPTHGQIYVLGQDISRVTPSRRAQLRRRIGIVFQDFRLLDHLSTWENVAL
                     PLRVTGKRVSDYKEDVTDLLQWVGLGDRMHSFPSVLSGGEKQRAAIARAVINKPELLL
                     ADEPTGNVDPQMARRLLRLFIELNRLGTSVVIATHDHQLMRQTKAPRLELHDGHVRIV
                     "
     gene            2357..3442
                     /locus_tag="VFR00_09590"
     CDS             2357..3442
                     /locus_tag="VFR00_09590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013949664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0159558.1"
                     /translation="MSGSYDRPTGPPTLDPRGSRLEHDFHGGPVTGPPTHVQRARASP
                     HQHAKAGPRARPRRGDHAQANAPIVPAGSVTGRSLTLVITIMCFLACLTAGAVYMMNQ
                     SASAWLRDIASEVTVQIEARDRADIDKVVADVASFLAAQPGIATVRPLSVADSNQLLE
                     PWLGQTDALRALPIPRLVALEVDRNAPAELDGLRQTLKERFKGVTLDDHRRWQQQIRT
                     ITRSFALGGLAILVLVGAATTAIIISATRSAMASNREIVEVLHFVGATDRFIAREFER
                     HFLRLGVRAGIVGAIWAMLVFLAMPTVMELLGGGAVTLAEIRRLVGIATLDPPGYFLL
                     GIVVLVIAALCMLTSRIGVYRILNVRQ"
     gene            3550..>3749
                     /locus_tag="VFR00_09595"
     CDS             3550..>3749
                     /locus_tag="VFR00_09595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HEU0159559.1"
                     /translation="MARPGRFIAAALGSALLAFLGGFVVFAGTITHYAPRPVEQADSI
                     VVLTGGPFRLAAAARLLADGAG"
BASE COUNT          666 a         1266 c         1179 g          638 t
ORIGIN      
        1 ccgggtgaaa gcgcacaagg acgatcgcgc cgagatcaac gccagcgcgc aaaccgaagc
       61 caagggcctg accgccaagc gtgcgagcga ccttttagca gactgcctca aaccgctagc
      121 cacccatgtc gccaaatcgc aaggtccagc cggcgcctcc gaccaggcca gtgccaaggc
      181 ctgggaagcg atcgatgagg tcgtcgcgac ggcgctgacc gcgaccaacg gcgccttgtc
      241 gcaggcgggc gccagattgg tgccgctcga tgcaccggcc tggcacgccg acgcccaggg
      301 acatcggctg ccacttgcca tcgaggttta tggcaaggac gtggcccgca tgcacatcga
      361 tcatctcggc gccgaggtcg aagtgtccgt cggcgtcccc gatgcgctct ttatcaatct
      421 cggccggcgc cggcgcattc ccgtcgccgg tcttgccacc cacccgctcg cggaggtgat
      481 tgccgattgc gcctggcccg tcatctcccg ctcccgggac agccgtcgct cggcctaaac
      541 gattgcgccg gaatggccga acgtacgcga tcgcaggctt ggcctgtttc acgtggaaca
      601 ggccgcgggc tcagcccagc gcgcctttga ccgccgttcg gatctgctcc aaggtgaagg
      661 gcttgctaat gatgcgggcg attttgctgc gcaaatgctc ggcacgtccg agctcgtcgg
      721 caaagccgga catcaagagg acgcgcagcc cgggggccac cttgaggctc gcctcgacca
      781 gggcgatgcc gtcgagaccc ggcatctgca cgtctgatat cagcatgtcc gggggcgcct
      841 gggcggcgcg caggtactcg agcgcctccg agccatcctg cgtaaccacc acctcgtgac
      901 cgtcaaggcc gagcgcacgc tgcaccagat cacgcgtggc ggcgtcatct tcggcgagaa
      961 ggatgcggac catgaaacct cctgctccga cctgcctcaa ggcgactgtc gcgttagccg
     1021 gcccacaaaa ggcagctcgc ggaagcggtg cgcgaggtcc ataccatagc ccacgacgaa
     1081 cacgttagga cactcaaatg cccgtatatc cgcagtaata ttggcggcgc gagcgaccgg
     1141 tttgtccaac agcacgcacg acaggacgcg ggcggcgccg cgggccatga tcaggtcctt
     1201 ggcaaaggca agcgtgcggc cggactccaa aatgtcatca atcaacagga cattacggcc
     1261 gtgcacatcg gtttcgatgt cgcgcaggat ctcgatgcgt cccagggatc gcgtgccggc
     1321 acggtaactg gagagcgaga taaaatcgac ctctggggac aggccaacgc gatagagggc
     1381 gcgaatcaag tcggccgcaa acacaaaact gcccttgagc acggccacgg ccagaaggtc
     1441 ctgcagaccg gccccggcga tatccgcggc caatgcttcg atgcgctcgg cgatcgcggt
     1501 ggcggaaaaa atcgtctcga tatgtgccgg cacgccattg tccataccac gcctcctggc
     1561 aagctctcct tagacaatgc cggggggcgg cggcaagcca agcctgcctg cgggcccggc
     1621 gaggcatcag tccgccacaa tcctggagga ttgatcgcta ttcccccgca cgacgataaa
     1681 ggctaaggag cccagatcag gaggccggca tacgacgtga ttaggctaaa cggcatcggc
     1741 gtgaattacg agggaggcaa ccatgtgttg cgcgatgcga gctttcattt gcgtcccggc
     1801 tcgttccatt tcctgaccgg tccgtccggc tcgggtaaga ccaccctttt gcgcctcttg
     1861 ttcatgtcca tgcagccgac acacgggcaa atctacgtct tgggccagga catcagccgc
     1921 gtcacgccgt cccggcgcgc gcagctgcgg cgccgaatag gcatcgtctt ccaggacttt
     1981 agattgttgg atcatctgag cacttgggag aacgtggctc tgcccctgcg cgttaccggc
     2041 aagcgcgttt ccgactacaa ggaggacgtc accgatctgt tgcagtgggt cggactgggc
     2101 gatcgcatgc actccttccc ctccgtcctg tcgggaggag aaaagcagcg cgcggccatt
     2161 gcccgcgccg tcatcaacaa gcccgagctg ctgcttgccg acgagccgac gggaaacgtc
     2221 gatccacaga tggcccgccg cctgctgcgc ctgtttatcg aactcaaccg gcttggcacc
     2281 tcggttgtga tcgccaccca cgaccaccag ctgatgcggc agaccaaggc gccgcggctc
     2341 gaactgcacg acggtcatgt ccggatcgta tgaccgcccg accgggccgc cgacgctcga
     2401 tcccaggggg tcgcgcctcg agcatgactt ccatggcggc cccgtcacgg ggccacccac
     2461 ccacgtccag cgtgcgcggg cctccccgca tcagcatgcc aaggccgggc cgcgcgcgcg
     2521 gccgcggcga ggtgaccacg cccaggccaa tgcgccaatc gtgcccgccg gctcggtgac
     2581 cggccggtcg ctgacgctcg ttatcaccat catgtgcttt cttgcctgcc tgaccgccgg
     2641 tgcggtctat atgatgaacc aatcggcatc cgcttggctg cgcgacatcg ccagcgaggt
     2701 gaccgtgcag atcgaggccc gcgaccgtgc cgacatcgac aaggtcgtgg ccgacgtggc
     2761 gagcttcctg gcggcccagc ctggcattgc cacggtgagg cccctgagcg tggccgactc
     2821 caaccaattg ctcgagccgt ggctcggcca aaccgacgcc ctgcgggcgc tgccgatccc
     2881 gcgcctggtc gcgctcgagg tcgaccgcaa cgctcccgca gagctcgatg gcttgcgcca
     2941 gacgctcaaa gagcgcttca agggcgtaac gctggacgac caccgccgct ggcagcagca
     3001 gatccgcacc atcacccgct cctttgccct cggcggcttg gccatcctgg tgctggtagg
     3061 ggctgcaacc accgcaatca tcatttcggc gacccgcagc gccatggcgt cgaaccggga
     3121 aatcgtcgag gtcttgcact tcgtcggcgc tacggatcgt ttcatcgccc gggagttcga
     3181 gcgccatttc ctgcggctcg gggtgcgggc gggcatcgtc ggcgccattt gggcgatgtt
     3241 ggtgttcctc gccatgccga cggtcatgga actgctcggc ggcggcgccg tgacgctggc
     3301 cgagatccgc cggctggtgg gcatagccac gctcgacccg ccgggctatt ttttgctcgg
     3361 catcgtggtc ctggtgatcg cggcgctttg catgctcaca tcgcgcatcg gcgtttaccg
     3421 tattttgaac gtgcggcagt aagcaacccc gcgtcggcgt tacgtgagac ggtgcccaat
     3481 cacaagtggg cccaaatcgg ctactttgct ccaccactac cggcgcgagc gtccggctgg
     3541 agaggtaacg tggcacgacc aggcaggttc atcgccgccg ctctaggttc cgccctcttg
     3601 gctttcctgg gagggttcgt ggtgttcgcc ggcacgatca cgcactatgc accccggcct
     3661 gtcgaacagg cagacagcat tgtggtgctg accggcgggc catttcgcct ggcggcggcg
     3721 gcgcgcctgt tggcggacgg agccggcaa
//
LOCUS       DASTMT010000412         8110 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_1868650, whole genome shotgun sequence.
ACCESSION   DASTMT010000412 DASTMT010000000
VERSION     DASTMT010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 8110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8110)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8110
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1868650"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..167
                     /locus_tag="VFR00_09600"
     CDS             <1..167
                     /locus_tag="VFR00_09600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018326959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="accessory factor UbiK family protein"
                     /protein_id="HEU0159560.1"
                     /translation="RAQGERLLRDLDLVQREEFEAVKEMAAKAREENERLAQRLAQLE
                     GEIAALRRPA"
     gene            401..898
                     /locus_tag="VFR00_09605"
     CDS             401..898
                     /locus_tag="VFR00_09605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HEU0159561.1"
                     /translation="MAIAEMTRTRTNNPISLLEQIAGSNDWAFDRGSEDELTLNVAGA
                     WTDYHVSLNWRLDLETLHIACAFDAKIPDNRLAEVYRLVAQINEQLWVGHFDVWTQEG
                     LIMFRQGLMLNGALATPHQCEALLRAALEACERYYQAFQFVVWAGKDSREALASTMFE
                     TEGQA"
     gene            1002..1823
                     /gene="proC"
                     /locus_tag="VFR00_09610"
     CDS             1002..1823
                     /gene="proC"
                     /locus_tag="VFR00_09610"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010969866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEU0159562.1"
                     /translation="MTLKTDGPLVLAGAGNMGAALLAGWLQRGLDPSAVLIQDPSPPP
                     ASLALLERHGLRASARLDAAAAPAVIVVAVKPQIMDEVFPALARHAGPRTVVLSIAAG
                     RTLAGFERHLEPSAAVVRAMPNTPAAIARGITVAVANAHVSPAQRQLCDALLRAVGEV
                     EWVDEEGLLDPVTAVSGSGPAYVFYLAECLAAAGVKAGLTPELAMKLARHTVAGAGEL
                     IARSGAAPAALRESVTSPNGTTFAALKVLMGQDGLERLLGEAVAAATRRSRELAK"
     gene            1893..2999
                     /gene="hisC"
                     /locus_tag="VFR00_09615"
     CDS             1893..2999
                     /gene="hisC"
                     /locus_tag="VFR00_09615"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HEU0159563.1"
                     /translation="MPNTQPVPQPGILEIEAYVPGESSVGGALKPIKLSSNETPLGPS
                     AQAVAAYKAAADTLALYPDGSAADLRRAIAGHYGLDPERIVCGSGSDELINLIAHAYI
                     GPGDEAIYSEHGFLMYKIATLSSGGKPISVPEKNYRADVDALLAAVTPKTRVVFLANP
                     NNPTGTYLPHDEVRRLHRGLPAHVLLLLDAAYSEYVRRNDYEAGLELVATTGNTVMTR
                     TFSKIYGLAALRLGWAYCPAAVADVLNRVRGPFNVTASALAAGKAAILDRAHLERAVA
                     HNEKWLPWVAAELARLGLEVTPSVGNFLLIHFPGDPARSAGAADEFLKARGIILRRVA
                     AYGLPGALRMTIGTEDDNRRVVASLKEFLGRAHD"
     gene            2992..3927
                     /locus_tag="VFR00_09620"
     CDS             2992..3927
                     /locus_tag="VFR00_09620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014132094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate/arogenate dehydrogenase family
                     protein"
                     /protein_id="HEU0159564.1"
                     /translation="MTEEVFERVALIGLGLIGSSLSHAIRRGGLAKSIVGHAQSEKTR
                     DTALRLGLVSSVYATPAEAVRQADLVILCTPIGAYASIATAIAPALDRGAIVTDVGSV
                     KGSVARDVGPFMPAGVHFIPGHPIAGTEHSGPHAGFAELFDGRWCILTPARDADPAAV
                     ERLKTFWMALGSHVEIMDAEHHDLVLAITSHVPHLIAYNIVNTARHLERVTDSEVIKF
                     SAGGFRDFTRIAASDPVMWRDVFLNNKEAVLEMLARFTEDLTALQRAIRFGEGASLHQ
                     LFTEARATRRGIIEAGQDTAAPDFGRHAGGKPSGT"
     gene            3924..4610
                     /locus_tag="VFR00_09625"
     CDS             3924..4610
                     /locus_tag="VFR00_09625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018646546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003839 - gamma-glutamylcyclotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0061928 - glutathione specific
                     gamma-glutamylcyclotransferase activity [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HEU0159565.1"
                     /translation="MTAPASEHWIFAYGSLMWRPGFPYEEARHARLIGYRRCFCIYSM
                     HHRGTPQRPGLVLGLDRGGTSEGIAYRISGANYAAIVRYLREREQVSGVYREVNVAVN
                     LLGGTAREVMALTYVVERAHPSYAGRLTLARQARLIRGASGLSGINLDYLISTLRHLA
                     ELGIRERDLERLIAMIGPHTARGRRPDRANASVLAMLRATRRLPVLVPVRGLKLDARR
                     RFLHRQGVLA"
     gene            complement(4625..5770)
                     /locus_tag="VFR00_09630"
     CDS             complement(4625..5770)
                     /locus_tag="VFR00_09630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018644102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEU0159566.1"
                     /translation="MRIAVLGGGPAGLYFAARWKRRHPGDLVRLWEQNPADATFGFGV
                     VFSQTALEFLRHRDAQTFALLGPHLESWRDITIVHRGQRITIDGVGFSAIGRLDLLRL
                     LQQAARAAGVQIKFGRPVNSLATLARFDLIVGADGVNSILRRELSDRFGTSVAELENP
                     FIWYGTERTFDTLTQTFVANAMGTFNAHHYRYQAGRSTFIVECDPATWRRAGFDRLDR
                     EGCRRLCEQVFAATLQGQPLIDNNSSWRHFPSVSNRCWSTGNVVLIGDAAHTAHYSIG
                     SGTRLALGDALALAETLEQAPPGQITAALQGYEAARRKSVESLVAASLRSAAWYARFP
                     EHMRLEPLELAMSYLTRSGAVDADRLRSTSPAFMAHYARHSATTHEA"
     gene            complement(5789..6748)
                     /locus_tag="VFR00_09635"
     CDS             complement(5789..6748)
                     /locus_tag="VFR00_09635"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012497734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HEU0159567.1"
                     /translation="MEPKPDDYDYELDRALLSVVGIRALVPADAFTAETLGTERRGNG
                     VLIRDDGLILTIGYLITEAETVWISHSGGRAVPGHVLGYDQETGFGLLQALARLDLPA
                     LPLGSSKTAEVGERVVVGGAGGRPRSIAARIIAKQEFSGYWEYVLDEAIFTAPSHPHW
                     GGTGLIGPEGDLIGIGSLQIQQVAANPSGRAQRQAEDANMIVPIDLLKPILDDMLTIG
                     RPNRPARPWLGLYATEVGNSVAILGIASRGPAANADLKAGDIVLSVAGTPVSTLAGLF
                     RQVWALGQAGVNVPLQIHRDGRTFDVTLRSSDRRRYLKGPVLH"
     gene            6934..7386
                     /locus_tag="VFR00_09640"
     CDS             6934..7386
                     /locus_tag="VFR00_09640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018647280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2214 family protein"
                     /protein_id="HEU0159568.1"
                     /translation="MAAFFAFLHHLAAFTLLAALIVEFVLLSDELTLRSARKILLADM
                     VLGIAAGVIFIVGLGRVFHFEKGPYYYFHNWAFIAKLSLFVLVAIASIVPTREFLGWR
                     SAVKSGRLPSVEPPRLRAIRSIIHLELAGIVIILLCAALMAKGIGTRV"
     gene            7509..7922
                     /locus_tag="VFR00_09645"
     CDS             7509..7922
                     /locus_tag="VFR00_09645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018265708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SxtJ family membrane protein"
                     /protein_id="HEU0159569.1"
                     /translation="MHQIEAHTAIAIGSERSFGLVLAAALTMVAAWPLLTGQDPRWTA
                     LALAAGFLVTARVNPPLLRPLNQLWFKFGLLLSRLTTPIVMGLLFFAVVVPTGIVMRL
                     SRKDLLSLKFDPAAPTYWVKRDPVGPPPDSMLNQF"
     gene            7962..>8110
                     /locus_tag="VFR00_09650"
     CDS             7962..>8110
                     /locus_tag="VFR00_09650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018642991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5989 family protein"
                     /protein_id="HEU0159570.1"
                     /translation="MLSFAGELWRFLRARKKFWMLPILIMMATVGGLVVLTQGSAVAP
                     FIYTI"
BASE COUNT         1367 a         2718 c         2592 g         1433 t
ORIGIN      
        1 tgcgggcgca aggcgagcgc ctcctgcgcg acctcgatct cgtgcagcgt gaggaattcg
       61 aagcggtcaa ggagatggcg gcaaaggcgc gcgaggaaaa cgagcgtctg gcccagcgtc
      121 tcgcccagct tgaaggcgag atcgcggcgc tgcgcaggcc ggcgtagccg cgccgcagcg
      181 caaagacgtt tcacagctaa tcgacagtct gtccccaact tttgccggcg ctggcccgca
      241 acttggcgtg gcctatttgt ccgtggacaa tcaggccgcc gtcttgcaac gcacgccggc
      301 atgcgattac gcagtaatgg gatcgaagca aacgccacag cttgactcga taaagactcg
      361 aacgcagcca tcgcgtgcga gtgaaaaaca gagggacgga atggccatcg ctgaaatgac
      421 gcgcactcgc acgaacaacc cgattagcct gctcgagcag atcgccggca gcaacgactg
      481 ggccttcgac cgtggcagcg aggacgagtt gaccctcaac gtggccggcg cgtggaccga
      541 ctatcacgtg tcgctcaatt ggcgcctcga tctcgagacg ctgcatatcg cctgcgcctt
      601 cgatgccaag atacccgaca atcgtttggc cgaagtctat cgcctggtgg cgcagatcaa
      661 tgagcaactg tgggtcggcc atttcgacgt ctggacccag gaaggcttga tcatgtttcg
      721 ccaggggctg atgctgaacg gcgccctggc aacaccgcat caatgcgagg ccctgctgcg
      781 ggccgcgctc gaggcctgcg aacgctacta ccaagccttc caattcgtgg tgtgggccgg
      841 caaggacagc cgggaagcct tggcctccac catgttcgag accgaagggc aggcctaagc
      901 ctttttgccg ccttcgacct cccaattcgt gccgcgcgac tttaagatgg cccacatcct
      961 tggccgggca ggtgcccgag cgttgaaagg ggcggtgctg catgaccctg aaaaccgatg
     1021 gcccgctggt gctcgccggt gccggcaaca tgggggcggc gttgttggcg ggctggctcc
     1081 aacgcggcct cgatcccagc gccgtgctga tccaggatcc ctccccgccg ccggccagtt
     1141 tggcgctact cgagcgccat ggcttgcgcg ccagtgcgcg cctcgatgcg gcggcggcgc
     1201 ccgccgtgat tgtcgttgcc gtcaagccgc agatcatgga cgaagtcttt ccggcgctgg
     1261 cgcgccacgc cggcccgcgc accgtcgtct tgtcgatcgc cgccgggcgc acgcttgcgg
     1321 gcttcgagcg ccatctcgag cccagcgccg cggtggtgcg ggcgatgccc aacacgccgg
     1381 ccgccattgc gcgcggcatt accgtggcgg tggccaacgc gcacgtcagc ccggcgcagc
     1441 gccaactttg cgatgccctg ctcagggccg tcggcgaggt cgaatgggtc gacgaggagg
     1501 gcctgctcga cccggtcacg gcggtatcgg gttcgggtcc ggcctacgtc ttctatttgg
     1561 ccgaatgcct ggccgcagcc ggcgtcaagg ccgggcttac gccggagctg gccatgaagc
     1621 tggcgcgcca caccgtcgcg ggggcaggtg aactcatcgc gcggtcaggc gccgcccccg
     1681 ccgccctgcg cgaaagcgtc acctccccca acgggaccac cttcgccgcc ctcaaggtgc
     1741 tgatgggcca agacggcttg gagcggctgc tgggcgaggc tgtggctgcg gccacccgcc
     1801 gctcgcgcga gcttgccaaa tagatcagcc agccgctggc caagtccctg gtctttgcct
     1861 attttgcgcc gcgcaaagac aagagcaccc ccatgcccaa cacgcaaccc gtgccgcagc
     1921 ccggcatcct ggagatcgaa gcctacgttc ccggtgaaag cagcgtcggc ggcgcgctca
     1981 aacccatcaa gctgtcctcc aacgaaacgc cgctcggacc aagcgcgcag gcagtcgccg
     2041 cctataaggc ggccgccgac acgctcgccc tctatcccga cgggagcgcg gcagacctgc
     2101 gccgggcgat cgccggccac tatgggctcg atcctgagcg tatcgtctgc ggctcgggtt
     2161 ccgatgagct gattaatctc atcgcgcacg cctatatcgg tccgggcgat gaggcgatct
     2221 acagcgagca cggcttcctg atgtacaaga tcgccacgct gtcgagcggc ggcaagccca
     2281 tttccgtccc ggaaaagaac tatcgcgccg acgtggacgc cctgctggcg gcggtcaccc
     2341 ccaagacccg cgtggtgttt ctggccaatc ccaacaatcc aacggggacc tacctgcccc
     2401 atgacgaggt ccggcgcctg catcgtggtc tgccggcgca cgtgctcttg ctgctcgatg
     2461 cggcctacag cgagtatgtg cggcgcaacg actacgaggc cggattggag ctcgttgcca
     2521 ccaccggcaa taccgtgatg acgcgcacgt tctcgaaaat ctacggcctt gccgcgttgc
     2581 gcctcggctg ggcctactgc ccggccgccg ttgccgatgt gctgaaccgc gtgcgcggcc
     2641 cgttcaatgt gacggcgtcg gccctggcgg cgggcaaagc ggccattttg gaccgcgctc
     2701 acctcgagcg cgccgtggcg cataacgaaa aatggctgcc ctgggtagcg gccgagctcg
     2761 ccaggctcgg ccttgaggtg acgcccagcg tcggcaattt cctgctcatc cactttccgg
     2821 gcgaccccgc ccgcagcgct ggcgcggcgg acgagttcct gaaagcgcgc ggtatcatcc
     2881 tacgccgggt cgccgcttat gggctacccg gtgcgctgcg catgacgatc ggcacggagg
     2941 atgacaatcg gcgcgtggtc gccagcctca aggaattcct ggggcgggcg catgactgag
     3001 gaagtttttg agcgcgtcgc cctgatcggg ctcggactga tcggttcatc cttgagccat
     3061 gccatacgtc ggggcggcct ggcgaagagc atcgtcgggc acgcgcaatc agaaaagacg
     3121 cgcgatacgg cgctgcggct tggattggtg agctcggtct acgccacccc ggccgaggcc
     3181 gtgcggcaag ccgatctcgt catcctgtgt acgccgatcg gcgcctacgc gtcgattgcc
     3241 accgcgatcg ctcccgccct cgatcggggc gcgatcgtta cggacgtggg ctcggtcaag
     3301 ggctccgtgg cgcgcgatgt cggacctttt atgccagcgg gcgtgcattt tattcccggg
     3361 catccgatcg ccggaaccga gcattcgggt ccgcacgcgg gttttgccga gctattcgac
     3421 gggcgctggt gcattttgac gcccgcgcgc gacgccgacc ccgcggcggt cgagcggctc
     3481 aagacctttt ggatggcgct tggcagccac gtcgaaatca tggacgcgga gcatcacgat
     3541 ctggtgctgg ccatcaccag ccacgtgccc catctcatcg cctacaatat cgtcaatacc
     3601 gcacggcacc tcgagcgcgt gaccgacagc gaggtgatca aattttccgc cggcggcttt
     3661 cgcgatttca cgcgcatcgc cgcctcagat cccgtcatgt ggcgcgatgt cttcctcaac
     3721 aacaaggaag ccgtgctgga gatgcttgcg cgtttcaccg aggatctgac ggcgctgcag
     3781 cgcgccatcc gcttcgggga aggcgcgagc ttgcaccagc tctttacgga ggcgcgcgcc
     3841 acgcggcgtg gcatcatcga ggccgggcag gacaccgcgg cacccgattt cggccggcac
     3901 gccggcggca aaccttccgg cacatgacgg cgccggcttc cgagcactgg attttcgcct
     3961 atggctcgtt gatgtggcga cccgggttcc cctacgagga agcccggcac gcgcgcttga
     4021 tcgggtatcg ccgctgcttt tgcatctatt ccatgcatca ccgcggcacc ccgcagcgac
     4081 ccggcctggt cctgggtctc gaccgtggcg gcaccagcga aggcatcgcc tatcgaattt
     4141 cgggcgcaaa ctatgccgcc atcgtgcgtt acctgcgcga gcgcgagcag gtgagcggcg
     4201 tctatcgcga ggtcaacgtg gcggtgaacc tgcttggcgg caccgcgcgc gaggtcatgg
     4261 cgctcaccta cgtggtagag cgcgcgcatc cgagctacgc cggccgtctg accctggccc
     4321 gacaggcgcg gctgatcagg ggcgccagcg ggctctccgg catcaacctc gactatctca
     4381 tcagcacgct gcgccacctt gccgaactcg gcatccgcga acgcgatctg gagcgcctga
     4441 tcgccatgat cggcccgcac acggcgcgcg ggcgccggcc cgaccgggcc aatgcctcgg
     4501 ttctggcgat gctgcgcgcc acccggcgcc taccagtctt ggtgcctgtg cgcggcctga
     4561 agctcgacgc acgccgccgc ttcctgcatc gccagggcgt tctcgcctga gcctgagccc
     4621 cgtctcaggc ttcgtgcgtc gttgccgaat ggcgggcata atgcgccatg aatgcaggcg
     4681 acgtcgacct tagccgatcc gcgtcgacgg cccccgagcg cgtcaggtaa ctcatggcaa
     4741 gctcgagcgg ctcgagccgc atgtgctcgg gaaagcgggc ataccacgca gccgaacgca
     4801 gcgaagcggc caccaggctt tccacgcttt tccgccgtgc cgcctcgtag ccctgcaacg
     4861 ccgcggtgat ctggccgggc ggggcctgct ccagggtctc ggccagagcc agtgcgtcgc
     4921 ccagcgccag ccgcgtgccc gagccgatcg agtaatgggc ggtatgcgcg gcatcgccga
     4981 tcaggacgac gttgcctgtc gaccagcatc tattgctgac agacgggaaa tgccgccagc
     5041 tcgagttgtt gtcgatcagc ggctgacctt gcaaagtcgc ggcgaacacc tgttcgcaca
     5101 gccggcggca gccttcccga tccagccgat cgaaaccggc gcgccgccag gttgccggat
     5161 cgcactccac gataaaggtg ctgcggcccg cttgatagcg gtaatgatgg gcgttgaacg
     5221 tgcccatggc attggcgacg aaggtctgcg tcagggtatc gaatgttcgc tcggtgccgt
     5281 accagatgaa gggattttca agctccgcca cgctggtacc gaaacggtca ctcagctccc
     5341 tgcgcaggat ggaattgacg ccgtccgcac cgacgatcag gtcgaagcgc gccagcgtcg
     5401 ccagcgagtt gacggggcgg ccgaacttta tctggacccc ggccgcacgc gccgcctgtt
     5461 gcaggagcct cagcaggtcg agccgaccga tggcggaaaa accgacgccg tcgatcgtaa
     5521 tgcgttggcc acgatgcacg atggtaatgt cgcgccaact ctcgaggtgc ggccccaaga
     5581 gcgcaaaggt ttgcgcgtcc cgatgccgca aaaactcaag cgcggtttgc gagaacacga
     5641 cgccgaaacc aaaggtggca tcggccgggt tctgttccca gagccgcacc agatcgccgg
     5701 ggtggcgccg cttccaacgt gcggcgaaat ataggcccgc ggggccgccg cccagcacgg
     5761 cgatgcgcat cagggccatc ctcgtggctc agtggagcac aggacctttg agataacggc
     5821 gccgatctga ggatcgcaag gtgacgtcga aagtcctgcc atcgcgatgg atttgtaagg
     5881 gaacattgac ccccgcctgg ccaagcgccc agacctgccg gaacaggccg gccagcgtcg
     5941 acaccggcgt gccggccaca ctcagaacaa tgtcgccggc cttgaggtcg gcattggcgg
     6001 ccggcccacg actggcgatg cccagaatgg ccacgctgtt gccgacctcg gttgcataaa
     6061 gaccgagcca ggggcgcgcc ggtcggtttg gccggccgat cgtcagcatg tcatccagga
     6121 tcggcttcaa caggtcgatc ggcacgatca tgttggcgtc ttcggcttgc cgctgggccc
     6181 ggccgcttgg attggccgcc acctgctgga tctgcagcga accgatgccg atgagatcac
     6241 cctccggacc gatcaagccc gtgccgcccc agtggggatg ggagggggcc gtgaaaatcg
     6301 cctcatcgag cacgtattcc caatagccgg agaactcctg tttggcgatg atgcgggcgg
     6361 cgatcgagcg tggccggccg cccgcgccgc ccaccacaac gcgctcgccg acctccgccg
     6421 tcttggagct tcccaacggc agcgccggca aatccagacg cgccagcgcc tgcagcaggc
     6481 cgaaaccggt ctcctggtca tagcccagca cgtgacccgg cacggcgcga ccgccgctgt
     6541 ggctgatcca gacggtctcg gcttcggtga tcaggtagcc gatggtcaag atcaggccgt
     6601 cgtcgcggat caggacgccg ttgcctctgc gctcggtccc gagcgtctcc gccgtgaagg
     6661 catcggcggg aaccagcgct ctgatgccca ctaccgataa cagggcccgg tccagttcat
     6721 agtcatagtc gtcaggtttt ggctccacag tcagaagacc tcttgctggc cgcaccagat
     6781 cgcactggcc gcgccggtat tttatcccgc cgacgctcgg gtggccacca tcgcctgcaa
     6841 caacagggct gtgcagcgca atgctgaggg cttgacgccc gctgtggagc ggggcttgct
     6901 cagctcaacg cgctggaggg ggagagcctt cgaatggcag cttttttcgc tttcctgcat
     6961 cacctggcgg cattcacgct gcttgccgcc ctcatcgtcg aattcgtgct gctcagcgat
     7021 gagctcacgc tgcgcagtgc gcgcaaaatt ctcctcgccg atatggtgct tggcatcgcc
     7081 gccggtgtca tcttcatcgt cgggcttggc cgcgtgttcc atttcgagaa agggccctac
     7141 tactatttcc ataattgggc gttcatcgcc aagctctccc tgttcgtgct ggtggcaata
     7201 gcttcgatcg tgccgacccg ggagtttctg ggctggcgca gcgccgtcaa aagcggccgg
     7261 ctgccgagcg tcgagccgcc gcggttgcgc gcgatccgct ccatcatcca tctcgagctc
     7321 gctggcatcg taatcattct gctttgcgcg gcgctgatgg ccaaggggat cggcacgcgg
     7381 gtgtaaaggc gctctcgaaa gggcacaagc gcggctgtcg cgggggcgcc ggcggcaacg
     7441 ccagcgagct cgagctcccg agagaccggc tggcaggttg gcgccgaagt tgcaggtgcg
     7501 ctgacaccat gcaccaaatc gaagcacata ccgccattgc catcggttcg gaacgaagtt
     7561 tcggcctcgt tctcgcggcg gccttgacga tggttgcggc ttggccgctg ctcaccggcc
     7621 aagatccgcg ctggacggcc ttggcgctgg ccgccggctt ccttgtgacg gcgcgcgtca
     7681 acccacccct gttgcgacct ttgaaccagc tctggttcaa attcggactg cttttgtccc
     7741 gcctgacgac gccgatcgtc atggggctgt tgttcttcgc cgtcgtcgtg ccgaccggca
     7801 ttgtcatgcg cctgtcgcgc aaggatctgc tgtccctcaa gtttgatccg gccgcgccca
     7861 cctattgggt caagcgtgac ccggttggac caccgcccga tagcatgctg aaccagttct
     7921 aaggtgccac ccacctcggc acgtaacctc ggagtaggac gatgctttcc tttgccggcg
     7981 aactttggcg atttctgcgc gcgcgcaaaa agttctggat gctgccgatc ctcatcatga
     8041 tggcaacggt tggcggcttg gtcgtgctga cgcagggctc ggccgtggcg ccgttcatct
     8101 acaccatctt
//
LOCUS       DASTMT010000413         4916 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_1871328, whole genome shotgun sequence.
ACCESSION   DASTMT010000413 DASTMT010000000
VERSION     DASTMT010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4916)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4916)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4916
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1871328"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            189..833
                     /locus_tag="VFR00_09655"
     CDS             189..833
                     /locus_tag="VFR00_09655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEU0159571.1"
                     /translation="MDEEAIKSAYRRWAPVYDRTFGFVAAEGRKHTVEIINRRKGRVL
                     EVGVGTGLSLPEYGRHLEIVGIDLSPDMLEKARERVVTERLRNVTGLHEMDAGDLKFD
                     DASFDTVVAMYVMTVVPDPEKVMRELSRVCRPGGEVLLVNHFSAEEGMRGWVERRMAP
                     FADMLGWRPVFDVERVLVCDDLVLMEKRGLRPFGLFTMMRFHKDKLAGQRPRAA"
     gene            complement(876..1895)
                     /locus_tag="VFR00_09660"
     CDS             complement(876..1895)
                     /locus_tag="VFR00_09660"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013167850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HEU0159572.1"
                     /translation="MTQSLETIAHAMVAPGKGILAADESSGTIKKRFDAIGVENTQSN
                     RRDYREMLFRSSEAMRSHISGVILYDETIRQQAKDGTPLVKVIAETGALPGIKVDTGA
                     KPLPFCAGETITEGLDGLADRIKDYVGLGAKFAKWRAVIDIGRNMPSYNAINANAQAL
                     ARYAALCVAGGLVPIVEPEVLMDGEHDIDTCEKMTEWVLKEVYAQLYYARVPLEATVL
                     KPNMVVPGKKCAKQASREEVAQRTVEVLRRCVPVAVPGIAFLSGGQSDEDATAHLSLM
                     NALGPLPWKLSFSYGRALQAAAQKAWAGKSENYAAGQRAFSHRAKMNGAAALGKWSQG
                     MEKAA"
     gene            2077..2610
                     /locus_tag="VFR00_09665"
     CDS             2077..2610
                     /locus_tag="VFR00_09665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013913230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HEU0159573.1"
                     /translation="MPEFTPPFRLAGALLGILVSLAAQAQSARLRIVALGASNTAGWG
                     VAAAEAYPAQLEALLAAKGMPATVLNAGIPGDTTGGMLARLEREVAPGTDLVILQPGT
                     NDERAGSGAERAGNIAEIERRLAARGTKLLIVENSMLAELPPSELREDGIHFTPQGYA
                     ILAQRVLPLLLEALPGR"
     gene            2715..3104
                     /locus_tag="VFR00_09670"
     CDS             2715..3104
                     /locus_tag="VFR00_09670"
                     /inference="COORDINATES: protein motif:HMM:NF013304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HEU0159574.1"
                     /translation="MTPELFYLVWSVALTVVLVVIAALGAMQQVGLPKLAGNREHMPE
                     ISGWGGRAERAHRNMLESLVLFAILVLAARALNVANSWTVLGAQLFFWGRLAHAVLYI
                     AGIPWLRTAAWLVSVLGLLLLFLQLVQ"
     gene            3281..4468
                     /locus_tag="VFR00_09675"
     CDS             3281..4468
                     /locus_tag="VFR00_09675"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0159575.1"
                     /translation="MPPSLPARQLITHVSAIGFATALSARALDPLIPSIAADLAVDAA
                     HVALLSTAFALPFACVQPILGPLADMVGKIRLMVTCLAIIIAVSLLSALAQSFTLLML
                     ARIVCGMATGGIFPVGLAIIGDAVPVGERQVAIARWLAITIGGNLIGAAFAGMVGDLL
                     GWRAVFWIIGGCGALALGNAAINLRGAAVRPPGRLDLSAIPSGYRGIFANPRAKFCFA
                     AVFLEGVAVFGLFPFVALLLVRAGEPRAAIAGLVIGGFSIGGVVYSLLVRLLMRRWQP
                     KRLMLGGGMIAAFAFLLVAANPPWPVQLTAFLVLGIGFYSLHGCIQVEATELSVSARG
                     AATSLHSLFFFLGHATGPVLYGLAFTRLGAGMAVVLGGLVMLGVGLMTAHTLGRPRPP
                     RAP"
     gene            complement(4494..>4916)
                     /locus_tag="VFR00_09680"
     CDS             complement(4494..>4916)
                     /locus_tag="VFR00_09680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HEU0159576.1"
                     /translation="MRRARAAALNMIPTSTGAAAAVGLVLPELKGKLDGTAIRVPTPN
                     VSVVDLKFIPGRETTADEINKAMEHASRQQLKGILAVTNEPLVSTDFNHDPHSSTFDL
                     TQTQIVDGKLVRVLSWYDNEWGFSNRMADTAVAIGKLL"
BASE COUNT          726 a         1613 c         1631 g          946 t
ORIGIN      
        1 ggaacaacgc gagcaagcgg atggggaagc cgcactgccc gcctaagtgg tgaagacgtc
       61 ggcgcccgct gacgatttgt gccaatgagg gagacgagta cgtcatgacg ttgacgccgc
      121 agtcgccgga gccgcgcgcg cagaagtttt ttaaacgttt cagagacgcc tctgagacgg
      181 gctcccagat ggacgaagag gccatcaaga gcgcctaccg acgttgggcc cccgtgtacg
      241 accgcacctt tggtttcgtc gccgccgagg gacgcaagca taccgtcgaa atcatcaacc
      301 gccgcaaggg acgcgtgctc gaggtgggcg tgggaaccgg cctgtccttg ccggagtacg
      361 gccgccattt ggagatcgtc ggtatcgacc tgtcgcccga catgctggaa aaggcgcgcg
      421 agcgggtggt gacggagcgt ctgcgcaacg tcacaggtct gcacgaaatg gatgcgggcg
      481 acctcaagtt cgacgacgcc tctttcgata cggtggtggc catgtacgtc atgaccgtgg
      541 tgcccgaccc cgaaaaggtg atgcgcgaac tgtcgcgcgt ttgtcgaccg ggcggcgagg
      601 tgctgctcgt caatcacttc agcgccgagg aggggatgcg tggttgggtg gagcgccgca
      661 tggcgccctt cgccgacatg ctgggatggc ggccggtctt cgacgtcgag cgtgtgctgg
      721 tgtgcgatga tctcgtgctg atggaaaagc gcgggctcag gcccttcggc ctcttcacca
      781 tgatgcgctt tcacaaagac aagctcgccg ggcaaagacc gcgcgccgcc tgagcagcgc
      841 gcaacccgaa cgcccaatgc aaagcggcgg cgtcgttacg ccgccttttc catgccctgg
      901 gaccatttgc cgagtgcggc cgcgccgttc atcttggcgc ggtggctgaa ggcgcgctgt
      961 ccggccgcat agttttccga tttgccggcc caggccttct gcgccgccgc ctgcagggcg
     1021 cgaccgtaag agaaggaaag cttccagggc agcggaccca gggcgttcat caaggacagg
     1081 tgcgccgtgg cgtcttcgtc cgactggccg ccggagagaa aggcaatgcc ggggacggcg
     1141 acgggcacgc agcggcgcag gacttcaacc gtgcgctgcg cgacctcctc acggctcgct
     1201 tgcttggcgc acttcttgcc cggcacgacc atgttgggtt tgagcacggt cgcctcgagc
     1261 gggacgcgcg catagtagag ctgggcataa acttccttca gcacccactc ggtcatcttc
     1321 tcgcaggtgt cgatgtcgtg ctcgccgtcc atcaagacct cgggctccac gatcggcacc
     1381 aagccgcccg caacgcagag cgctgcatag cgcgccaagg cttgtgcgtt ggcgttgatc
     1441 gcattgtagg agggcatatt gcggccaatg tcgatgacgg cgcgccactt ggcgaacttg
     1501 gcaccgagac cgacatagtc cttgatgcgg tcggcgagac catcgaggcc ctccgtgatg
     1561 gtctcgcccg cacaaaacgg caagggcttg gcgcccgtgt cgaccttgat gccgggcagc
     1621 gcgcccgttt cggcgattac cttgaccagc ggcgtgccgt ccttggcttg ctggcgaatg
     1681 gtctcgtcgt agaggatgac gccggagata tggctgcgca tcgcctcgct ggagcggaac
     1741 agcatctcgc ggtaatcgcg ccggttggac tgcgtgttct ccaccccaat ggcatcgaaa
     1801 cgtttcttga tggtgccgga gctttcgtcg gcggcgagaa tacccttgcc gggcgcgacc
     1861 atggcgtgcg cgatggtctc gagcgattgg gtcatgcttt cctcctccat gctcactggg
     1921 tcctgctgag agcggctgtg gttgcattag cgacatttag gcggcccgac cagggggctc
     1981 ggcgtggcgg gcgtgctata gaactcggcg ccgcgcacca ccgttcgccg acttttgcgc
     2041 gcaatcccat gacccgctgc ggccggaggg ggaacaatgc cagaatttac cccacccttc
     2101 cgcctcgccg gcgccctgct gggcatcctc gtgagcctgg ccgcccaggc acaaagcgcc
     2161 aggctcagga ttgtggcgct gggcgccagc aacaccgcgg gctggggcgt ggctgccgcg
     2221 gaggcctatc cggcccagct cgaggcgctg cttgccgcta agggcatgcc ggcaacggtg
     2281 ctcaatgccg gcattcccgg cgacaccacg ggcggcatgt tggcccgcct cgagcgcgag
     2341 gtggcgcccg ggaccgacct cgtcatcctg cagcccggga ccaatgacga gcgcgccggc
     2401 agtggcgccg agcgggccgg aaacattgcc gagatcgagc gccgattggc ggcgcgcggc
     2461 accaagctgc tgatcgtcga gaacagcatg ctggccgagc tgcccccgag cgaactgcga
     2521 gaggacggta ttcacttcac cccgcaaggc tatgccatct tggcgcaacg tgtcctgcca
     2581 cttctgctgg aggcgctccc cgggcgctaa cagcgccgag gaccgcaacg cacacctgcc
     2641 tactcgacag cccaaaagaa tatggctttc gtcttctcca ggtcgattcg ccgtcggaga
     2701 gggaggccgt gcgcatgaca ccagagctct tctatctcgt gtggtccgta gccttgaccg
     2761 ttgtgctggt cgtgatcgcc gcattggggg cgatgcagca ggttggcttg cccaagcttg
     2821 ccggcaaccg cgagcacatg cccgaaattt cgggctgggg cggacgcgcg gaacgggccc
     2881 accgcaatat gttggaaagc ctggtgctgt tcgccatcct ggtgctggcc gcgcgcgccc
     2941 tcaacgtcgc caatagctgg accgtgctcg gggcgcagct gttcttctgg ggccgcctcg
     3001 cccatgccgt cctctacatc gccggcatcc cctggctgcg aacggcggcc tggctggtct
     3061 cggtgctcgg gctgttgctc cttttcctgc agctcgtgca gtaagcctcc ggccgtgccg
     3121 gcattggcgt ggcgcgcgcc gaggcaccgc tccgtcgcgc cgactgtggc aatgtgcgct
     3181 gcccaaacgg gccgggcggc gccattgcgc aaggcaaccg gcatgacgct ggcgaagcct
     3241 tgggctattg ggcttccgcc agccctcgcc attgcccccc atgccgccct ccctgcccgc
     3301 ccgtcagctc atcacgcatg tcagcgccat cggctttgcg acggccctct ccgcgcgcgc
     3361 gcttgatccc ctgatcccct ccattgccgc cgatctggcg gtcgatgccg cgcacgtggc
     3421 gctcttgtcg acggccttcg ccttgccgtt cgcgtgcgtg cagccgattc tgggaccgct
     3481 cgccgacatg gtgggcaaga tccgcctgat ggtgacgtgt ttggccatca tcatcgccgt
     3541 atcgttgctg agtgccctgg cgcaaagctt cacccttctc atgctggccc gcatcgtctg
     3601 cggcatggcc acgggcggca tctttccggt gggcctcgcc atcatcggtg atgccgtgcc
     3661 ggtcggcgag cggcaggtgg cgattgcccg ctggctggcg atcaccatcg gcggcaacct
     3721 gatcggcgcc gcgtttgcgg gcatggtcgg agatttgctg ggttggcgcg cggttttttg
     3781 gatcatcggc ggctgcggcg cgctggcgct ggggaacgcg gcgatcaacc tcaggggcgc
     3841 cgccgttcgc ccgccgggcc ggctcgatct ctccgccata ccctcgggct accgcggcat
     3901 ctttgccaat ccgcgtgcca agttttgctt cgccgccgtg ttcttggagg gtgtcgctgt
     3961 cttcggatta tttccctttg tggctctgct gctcgtcagg gccggcgagc cgcgcgcggc
     4021 gatcgccggt ctcgtcatcg gcggcttttc catcggtggc gtcgtctatt cgctgctcgt
     4081 gcgcctgctg atgcggcgct ggcagcccaa acgcctgatg cttgggggcg gtatgattgc
     4141 cgccttcgcc ttcctgctcg tggccgccaa cccgccctgg cctgtacagc tgaccgcctt
     4201 cctggtgctg ggcattggct tttattcgct gcacggctgc attcaggtgg aggcaacgga
     4261 gctctcggtc agcgcgcgcg gggccgccac gtcgctgcat tcgctcttct ttttcctcgg
     4321 ccacgcgacc gggcccgtgc tctatgggct cgccttcacc cggctgggtg cgggcatggc
     4381 ggtcgttctc ggtggccttg tcatgctggg cgtcggcttg atgacggcgc acaccttggg
     4441 ccggccgcgc ccgccccgcg cgccctaaca gagaatggcc gccgcgcccg cggctacaac
     4501 agcttgccga tcgccacggc ggtgtccgcc atgcggttcg agaagcccca ctcgttgtcg
     4561 taccacgaca gcacgcgcac cagcttgccg tccacgattt gggtctgggt aagatcgaag
     4621 gtcgaggaat gcgggtcgtg gttgaaatct gtcgagacca gcggctcgtt cgtgaccgcg
     4681 aggatgcctt tgagctgctg tctggaggca tgctccatcg ccttgttgat ctcatctgcc
     4741 gtggtttctc ggccggggat gaatttcagg tccaccacgg agacattcgg cgtcggcacg
     4801 cggatggcgg tgccgtcgag cttgcccttc agctccggta gcaccagtcc gaccgccgcg
     4861 gcggcaccgg tggaggttgg aatcatgttc aatgccgccg cgcgcgcgcg ccgcat
//
LOCUS       DASTMT010000414         4845 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_3724262, whole genome shotgun sequence.
ACCESSION   DASTMT010000414 DASTMT010000000
VERSION     DASTMT010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4845)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4845)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4845
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3724262"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..191
                     /locus_tag="VFR00_09685"
     CDS             <1..191
                     /locus_tag="VFR00_09685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020088065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ergothioneine biosynthesis protein EgtB"
                     /protein_id="HEU0159577.1"
                     /translation="PGYRPPAGALGEYNGKFMVSQQVLRGGSCATPDGHTRATYRNFF
                     YPHQRWQFMGLRLATEIV"
     gene            191..1162
                     /gene="egtD"
                     /locus_tag="VFR00_09690"
     CDS             191..1162
                     /gene="egtD"
                     /locus_tag="VFR00_09690"
                     /EC_number="2.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020088066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030745 - dimethylhistidine
                     N-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-histidine N(alpha)-methyltransferase"
                     /protein_id="HEU0159578.1"
                     /translation="MLLNPNMRELLAERFALDDELASAVLGGLSRPQKTLPCRFFYDA
                     RGSELFEEITRLPEYYPTRTEMGILKAGAQAMVERLPPGGVLIEFGSGSSLKTEILLD
                     RLPGLAAYVMIDVSETALLGAQQRLARQRPALDVRAIVGDFSRPVPLPEDLRARPKMG
                     FFPGSTIGNLVPSEATSLLRSFRTALAPAGHLIIGADLKKDARRLVLAYNDAAGVTAA
                     FNLNLLARINRELGADFDLAGFRHEATYNPRHGRIEMHLVSLRQQSVTIRGHRFAFGE
                     GETIHTENSYKYTIDQFRELARSAAWRPRRVWTDAAEMFSVHELLAS"
     gene            complement(1380..1455)
                     /locus_tag="VFR00_09695"
     tRNA            complement(1380..1455)
                     /locus_tag="VFR00_09695"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1420..1422),aa:Ala,seq:cgc)
     gene            1598..1894
                     /locus_tag="VFR00_09700"
     CDS             1598..1894
                     /locus_tag="VFR00_09700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008597209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEU0159579.1"
                     /translation="MLPILYFIDQYLIDLFIWIIIGSAILSWLIAFNVVNPYNQFVRS
                     LWDLFNRITEPFLRPIRRMLPDMGGIDISPVVLILILVFIRVVIIRGWLMPLFS"
     gene            1891..2226
                     /locus_tag="VFR00_09705"
     CDS             1891..2226
                     /locus_tag="VFR00_09705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013892368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 family protein"
                     /protein_id="HEU0159580.1"
                     /translation="MTSAAERHPNAWATVAGGLKLRVRVTPKSGQDRVEGLIPGVDGP
                     LLRVRVRALAQEGAANAALTRLVAAWLGVPPGAVALIGGPKARIKTLMVRGDGPELAT
                     RAAARLAAT"
     gene            2245..3111
                     /gene="folD"
                     /locus_tag="VFR00_09710"
     CDS             2245..3111
                     /gene="folD"
                     /locus_tag="VFR00_09710"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006023415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HEU0159581.1"
                     /translation="MAAHIIDGRAIAEALRLKVAREVARLREAHRLIPGLAVILLGDD
                     PASQVYVATKAKQTRAAGMQSFELRLAADTPEDRLLGLIAELNQRQDVHGILLQLPLP
                     KHIAPLRVIEAIDPAKDVDGFHPINVGRLVTGAKGFAPCTPTGCVLLAKSVRSELGGL
                     EAVIVGRSNIVGKPMASLLLQENCTPTIAHSHTRDLAAVVRRADLVVAAAGRAELIKG
                     AWIKPGAIVIDVGIQRVADGNGKTRLVGDVEFVAAATVAAAITPVPGGVGPMTVACLL
                     QNTLRAAESTLG"
     gene            complement(3235..3534)
                     /locus_tag="VFR00_09715"
     CDS             complement(3235..3534)
                     /locus_tag="VFR00_09715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019170910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HEU0159582.1"
                     /translation="MAKKGYWIGHINITNPERYQDYIAANAIAFAKYRARFLVRAGEC
                     TVAKGDLDGHRHVVIEFADYATAKACHASPEYQAAAKIRDEASDGHLMIIEGYEA"
     gene            3783..4643
                     /locus_tag="VFR00_09720"
     CDS             3783..4643
                     /locus_tag="VFR00_09720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010910806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HEU0159583.1"
                     /translation="MRYEAPSSLEQAVGLLAAATGDARVLAGGTDLLVQMRTDILNPE
                     LVIDIKRIPELREIKEEGGGFRIGAAATGAELKEHPRLKSVWPGVVEAANLIGSTQIQ
                     GRATMGGNLCNGSPAADSVPALIAAGAKASVVGPNGRRDIAVEDVMLAPRKLSLTKGE
                     IIASFLLPAKSKGSADAYLRFIPRTEMDIAVVGCGVCLSLAGDGTISRARVGLGAVAA
                     RPLLVEEAAKALLGSKLEDAALSKLDAAAGAACKPIDDKRGTKEYRIKVAGVLARRAA
                     AIAYERARKA"
     gene            4643..>4845
                     /locus_tag="VFR00_09725"
     CDS             4643..>4845
                     /locus_tag="VFR00_09725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010910805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEU0159584.1"
                     /translation="MAKHHITATVNGDPVEFLCEPQETLLDALRDQLNLTGTKEGCST
                     GDCGACSVTVDDRLVCSCLMLGV"
BASE COUNT          813 a         1581 c         1595 g          856 t
ORIGIN      
        1 atcccggtta ccgcccgccg gcgggagcgc tcggtgagta caatggcaaa ttcatggtga
       61 gccaacaggt tctgcgggga gggtcgtgcg caacgccgga cggccacacg cgcgccacct
      121 atcgcaactt tttctacccc caccagcgct ggcaattcat gggcctgcgt cttgccacgg
      181 agatcgtctg atgctgctaa atccgaatat gcgtgagctg ttggcagaac ggtttgcgct
      241 cgacgatgaa cttgcgagcg ccgtactcgg cggactctcg cggccgcaga aaaccctgcc
      301 gtgccgcttt ttttatgacg cgcgcggcag tgaactgttc gaggagatca cgcgtctgcc
      361 ggagtactat ccaacgcgca ccgagatggg catcctcaag gcgggggcgc aagcaatggt
      421 tgaacgcttg ccgcccggtg gtgtgctgat cgaattcggt tcgggctcta gcctcaagac
      481 agaaatcctg ctggaccggc tgcctggcct ggccgcctac gtcatgatcg atgtctccga
      541 gacggcgctc ctcggcgcgc agcagcgatt ggccaggcag cgccccgcac tcgatgtgcg
      601 cgcgatcgtc ggcgactttt ctcgccccgt cccgctgccc gaagatttgc gggcacgccc
      661 gaagatgggc ttcttccctg gttcaaccat cggcaacctg gtgcccagtg aagccaccag
      721 cctgctgcgc agcttccgca ccgcgctggc gccggcgggc cacctcatta ttggggccga
      781 cctcaaaaag gacgcgcgcc ggttggtcct cgcctacaac gacgcagccg gggtcacggc
      841 ggccttcaat ctcaacctgc tcgcgcgcat caaccgtgaa cttggcgccg atttcgatct
      901 tgcgggcttc cggcacgagg cgacctacaa tccgcgccac ggccgcatcg aaatgcacct
      961 ggtcagccta cgccagcaga gtgtcacgat ccgaggtcac cgctttgcct tcggggaggg
     1021 cgagaccatt cacacggaaa actcctacaa gtatacgatc gaccagttcc gcgagctggc
     1081 gcgctcggcc gcatggcggc cgcgacgcgt ttggacggac gcggctgaga tgttcagcgt
     1141 gcacgagctc ctggccagct gaaccggccg ccacgcggcg ccgcagcttg cccgcggcgc
     1201 cctggcgccg atttgcttgg ggaaggcctt gcgcctcttc gctggtcgag ggctggcgcc
     1261 tggcgacccg cgtcgccggc gagccggcgt gcggcccccc cgttggccag cgccggcgcg
     1321 gcggcttgct ggttcggaat taagcttctg attctaaaga atatttaggc ggcctttggt
     1381 ggtggagccg aggggaatcg aacccctgac ctctgcagtg cgattgcagc gctctcccat
     1441 ctgagctacg gccccgcaag gccgggccat ttacgtggcg ggcccgcccg ctgtcaagga
     1501 tggccgcgcg cggcagggac ggggcgagcg ctcccttgcc gccgcacggc gctctcgtct
     1561 aaatctgcgg gcgaatcgcg acgagagagg tcacgggatg ctgccgatcc tatacttcat
     1621 cgaccagtac ctgatcgatc tattcatctg gatcatcatc ggcagcgcca ttctgagctg
     1681 gctcatcgcc ttcaatgtcg tcaatcccta caatcagttc gtgcgctcgc tttgggatct
     1741 tttcaatcgg attaccgagc ccttcctgcg gccgatccgg cgcatgctgc ccgacatggg
     1801 cggcatcgac atctcgcccg tggtgcttat tttaatcctg gtcttcatac gcgtggtcat
     1861 tatccgcggc tggctgatgc cgctgttcag ttgacctcgg cggccgagcg ccacccaaac
     1921 gcttgggcga cggtcgccgg tggcctcaaa ctcagggtgc gagtgacgcc caagtccgga
     1981 caggaccgcg tcgaggggct gatacctggc gtggacggcc cgctgctcag ggtacgggtg
     2041 cgtgcgctcg cgcaggaggg ggctgccaac gcggctctca cccggctggt cgcggcgtgg
     2101 ctgggcgtgc cgccaggtgc ggtggcgctg atcggcggcc ccaaggcccg aatcaagacc
     2161 ctgatggtga ggggtgacgg ccccgagctc gccacgcgcg cggcggctcg tttggcagcg
     2221 acctgagtgc gcacgggagg ttgaatggcc gcgcacatca tcgatggacg ggcgatcgcg
     2281 gaggcgctgc gtctcaaggt ggcccgcgag gtcgcacggc tgcgcgaagc gcatcggctg
     2341 atccctgggc tagccgtcat attgctgggc gatgaccccg ccagccaggt ctacgttgcc
     2401 accaaagcca agcagacgcg cgcggcaggg atgcagtcat tcgagcttcg gcttgccgcg
     2461 gatacgcccg aggacaggct gctggggtta atcgccgaac tcaaccagcg ccaggacgta
     2521 catggcattt tgctgcaact gccgctgccc aaacacatcg cgccgttgcg cgtcatcgag
     2581 gcgatcgatc cagccaagga cgtcgatggc ttccatccca tcaacgtcgg gcggctggtc
     2641 acgggcgcca agggttttgc gccgtgcacg ccgacgggct gcgtgctgct ggccaagtcg
     2701 gtgcgctccg aactcggcgg cttggaagcc gtgattgtcg gccgctccaa tattgtcggc
     2761 aagccgatgg cctccttgct tttgcaagaa aactgcaccc ccaccatcgc ccactcgcac
     2821 acgcgggact tggccgccgt cgtccgccgg gccgacctgg tggtggccgc agcgggacgc
     2881 gccgagctca tcaagggagc ttggatcaag cccggtgcca tcgtcatcga cgtcggcatc
     2941 cagcgcgtcg ccgacggcaa cggcaagacg cggctggtcg gcgacgtcga gttcgtggct
     3001 gcggcgacgg tggcggcggc aatcacaccc gtgcctggcg gcgtggggcc gatgacggtc
     3061 gcctgtctgc tgcaaaatac gctcagggca gccgaaagca ccctcggctg accacgccga
     3121 cgccgtcagc cacacgggtt aggccgcagc aagcgcgccg ccgcagccag gaggagaggt
     3181 cgtcgcgccg ccgccggacg ccttggcaca cgaccccttg ccgtcagcgt ccggtcaagc
     3241 ctcgtagccc tcgatgatca tgaggtgccc gtcgcttgcc tcgtcgcgga tcttggcggc
     3301 ggcctgatac tctggggagg cgtggcaagc cttggccgtg gcatagtccg caaactcgat
     3361 gacgacgtgg cggtggccat cgagatcgcc cttggccacg gtgcattcgc cggcacgcac
     3421 cagaaagcgc gccctatact tggcaaaagc aatggcattg gccgcgatat agtcttggta
     3481 acgctcgggg ttggtaatgt tgatgtgacc gatccaataa cccttcttcg ccattttcac
     3541 ctccatcgcg acgatttgtc aggtccaatt cagcaaacca ggcggcccgt gggcaagcag
     3601 atggtggcgc gacccccgtg cgtctttgcc gcgcgcgaac ccgttgcccc cggccgtggc
     3661 gcgccaggcg ggcaggcggc aggagtggcc cctttccgcg ccgccgccga cgcgaatggg
     3721 tggcgcgagc caatgcagta tgccatgctt gcccggcgat aatcgaaccc gagaggaatg
     3781 cgatgcgata cgaggcgccc tcatcgctag agcaggctgt cggcctgctg gcggcggcca
     3841 cgggcgatgc acgagtcctg gctggcggca ccgatctgct ggtccaaatg cgaaccgata
     3901 ttctgaaccc tgagctggtg atcgacatca agcgcatccc ggagctgcgc gagatcaagg
     3961 aagagggcgg cggttttcgc atcggcgccg ccgcgacggg tgccgagctc aaggagcatc
     4021 cgcggctcaa gagcgtttgg cccggcgtcg ttgaggccgc caatctgatc ggctcaacgc
     4081 agatccaggg tcgagccacc atggggggca acctgtgcaa cggctcgccg gctgccgaca
     4141 gcgtgccagc gctgattgcg gccggggcca aggcctcggt ggtcggccca aacggccggc
     4201 gcgacatcgc cgttgaggat gtaatgcttg ccccccgcaa gctgtcgctg acgaagggcg
     4261 agatcattgc ctccttcctg ctgccggcga agtccaaggg cagcgccgat gcctacttgc
     4321 gttttattcc gcgcaccgag atggatatcg ccgtggtcgg ttgcggggtc tgcctcagcc
     4381 tcgctggcga tggcacgatt tcgcgggccc gggtcgggct tggggccgtc gcggcccgcc
     4441 cgctgttggt ggaggaggcc gctaaggccc tcctcggcag caaattggag gacgccgcgc
     4501 taagcaagct cgatgcggcg gcgggggccg cctgcaaacc gatcgacgac aagcgtggca
     4561 ccaaggaata ccgcatcaaa gtcgcgggcg tcctcgcccg ccgagctgca gcaatcgcct
     4621 atgaacgcgc gaggaaggcc tgatggcgaa acatcatatt accgcaaccg tcaatggcga
     4681 cccggtcgaa ttcctgtgcg agccacagga aacgctgctc gacgcgttgc gtgatcagct
     4741 gaatctcacc ggcacgaaag agggctgctc cacgggcgac tgcggcgcct gtagcgtcac
     4801 cgtcgatgat cgtctcgtct gctcctgtct gatgctggga gtgga
//
LOCUS       DASTMT010000415         2968 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_3511397, whole genome shotgun sequence.
ACCESSION   DASTMT010000415 DASTMT010000000
VERSION     DASTMT010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2968)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2968)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2968
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3511397"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            191..739
                     /locus_tag="VFR00_09730"
     CDS             191..739
                     /locus_tag="VFR00_09730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013950220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyloxyacyl hydrolase"
                     /protein_id="HEU0159585.1"
                     /translation="MRIAGLALAVLVLAVCGGEVAGAEELLRSGLEVRGGVLAHDVPD
                     LWSGFRLERGIDINGELLFGAGLPILGGSLRPALGGSVNTEGYTSKAYADLRWEMELP
                     AHLFLGLGLGAALHDGTLGPSEVDRKALGARVLFHIPLELGVRLDARQSLSVYFEHTS
                     NAGTARYNEGLDNIGLRYGYRF"
     gene            complement(842..1927)
                     /locus_tag="VFR00_09735"
     CDS             complement(842..1927)
                     /locus_tag="VFR00_09735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018321380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent alcohol dehydrogenase"
                     /protein_id="HEU0159586.1"
                     /translation="MKAAVLYEVNKPLVIEEVSINKPGPREVLLRTAYAGLCHSDLHF
                     IEGLYPHPLPVVLGHESAGIVEAVGSEVSYVKKGDHVITCLSVFCGTCENCTTGRPAI
                     CTNTQVKLPPGAAKRLSLDGKPLHTFTNLSSYAEQMLVHEHAVVKIRDDFPLDRAALI
                     GCGIITGYGAVVNTAKVAPGESVAVIGCGGVGMAAINGAAIAGAGRIIAVDTNPVKLQ
                     LATKLGATDLVNPRDGDPVQRIKDLTRGGVHHAVECLGTKATAEQAFQMLAVGGTATI
                     VGMVPFGQKLELHGFDFLRERKIQGSSMGSNRFRVDMPRLIDFYLQRKLHLDDWISDR
                     IALSQINEGFANMKAGKVVRSVIAFDA"
     gene            complement(1939..2523)
                     /gene="pcaG"
                     /locus_tag="VFR00_09740"
     CDS             complement(1939..2523)
                     /gene="pcaG"
                     /locus_tag="VFR00_09740"
                     /EC_number="1.13.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009737138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018578 - protocatechuate 3,4-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0046278 - 3,4-dihydroxybenzoate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protocatechuate 3,4-dioxygenase subunit alpha"
                     /protein_id="HEU0159587.1"
                     /translation="MPERTPSQTVGPYLHLGLAPAAYNVRPLFGPDLSAPGVTGTPIR
                     IEGRLIDGEGNGLPDALLEIWQADAQGHYAHPADGRPLRSNSFRGFARAPTDAGGGFA
                     FSTIKPGAVPGPSESLQAPHINVGIFARGLLKRLFTRIYFKDEGMNGSDPILALVPAP
                     RRHTLMAVPDGGRAGVFRFDIRLQGQDETVFFDA"
     gene            complement(2516..>2968)
                     /locus_tag="VFR00_09745"
     CDS             complement(2516..>2968)
                     /locus_tag="VFR00_09745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018455903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protocatechuate 3,4-dioxygenase subunit beta"
                     /protein_id="HEU0159588.1"
                     /translation="PVPETLVEIWQCNAAGRYIHVVDQHPAPLDPNFTGAGRCVTDAN
                     GVYEFITIKPGAYPWRNHANAWRPAHIHFSLFGPSFLTRLVTQMYFPGDPLFPFDPIF
                     NAVSDATARGRMVSQFDLATTRPEWALGYTFDIVLRGPNATPPDPDHA"
BASE COUNT          496 a          940 c         1027 g          505 t
ORIGIN      
        1 accggccgca acgcccgcaa attgggcctg ggctggcagt tttgccacgc ccgcaggcgg
       61 gaaatggcgg gccgcgcacg gctgcggctt ggcgccccgg caacggctca cctatgctgg
      121 gtcgggagcc gctgtcgcgc cgcccgaacc agggttttca ttgccttagc agctcgcgga
      181 gacgaccagc ttgcgcatcg ccggtcttgc ccttgccgtc ctcgtcctcg ccgtctgcgg
      241 cggggaggtc gccggcgccg aagagctcct gcgctcgggc ttggaggttc gcggcggcgt
      301 gctggcgcac gacgtgccgg acctgtggag cggttttcgg ctcgagcgcg gcatcgacat
      361 caatggcgag ctcttgttcg gcgccggcct accgatcctc ggcggcagcc tgcgcccggc
      421 gctcggcggc tcggtgaata ccgagggcta taccagcaag gcttacgccg atctgcgctg
      481 ggagatggag ttgccggccc acctgttcct gggccttggc ctcggcgcgg ccctgcacga
      541 tggcaccctc gggcccagcg aagtcgaccg caaggccctg ggggcgcggg tgctgtttca
      601 catcccgctg gaattgggcg tgcggctcga tgcccggcaa agtctgtcgg tctatttcga
      661 gcacacctcc aacgccggca ccgcgcgcta caacgagggt ctcgacaaca tcgggctgcg
      721 ctacggctac cgcttttgag gcgcacccag cctgcccgcg cgcggcagct cgcggcgggc
      781 ggcagcctgg gcacgacctg ccagaaaaaa gccccggggg atgccgcgcg agcctttgtc
      841 cttaggcgtc gaaggcgatg acactgcgca cgaccttgcc cgccttcatg ttggcaaagc
      901 cctcgttgat ctgcgaaagc gcaatgcgat cggaaatcca gtcgtcgagg tgcagcttgc
      961 gctgcaggta gaagtcgatc agcctcggca tgtcgacgcg gaagcgattg gagcccatcg
     1021 aggagccctg aatcttgcgc tcgcgcaaaa agtcaaagcc gtgcagctcg agtttttgtc
     1081 caaacggcac cataccgacg atggtggccg tgccgccgac ggccagcatc tgaaaggcct
     1141 gttcggccgt ggctttggtg ccgaggcatt ccacggcatg gtggacgccg ccgcgcgtga
     1201 ggtccttgat gcgctggacc ggatcaccat ccctggggtt gacgagatcg gtggcgccga
     1261 gcttggtggc gagttgcaac ttcacgggat tggtgtcgac ggcaatgata cgtccggcgc
     1321 cggcgatcgc cgcgccattg atggcggcca tgccgacccc accgcagccg atgacggcga
     1381 cgctttcgcc cggtgcgacc ttggccgtgt tcacgaccgc gccatagccg gtgatgatac
     1441 cgcagccgat cagcgcggca cggtcgagag gaaagtcatc gcggattttg acgaccgcgt
     1501 gctcgtgcac cagcatctgt tcggcgtagg aggacaggtt ggtaaaggta tgcagcggct
     1561 tgccatcgag gctgagccgc ttggcggcgc ccggcggcag cttgacctgc gtgttggtgc
     1621 aaattgccgg ccggccggtg gtgcagttct cgcaggtgcc gcagaatacc gacaggcagg
     1681 tgatgacatg gtcacctttt ttcacgtagc tgacctcgga gcctaccgcc tcgacgatcc
     1741 ccgccgactc atggccgaga acgaccggca atgggtgcgg atagagaccc tcgatgaagt
     1801 gcagatcgga atggcacaga cccgcgtagg ccgtgcgcag cagcacctcg cgcggccccg
     1861 gcttgttgat gctcacctcc tcgatgacga gcggcttgtt cacctcgtag agaaccgcgg
     1921 ctttcatttt ctcctccctc aggcgtcgaa aaacaccgtc tcgtcctggc cctgcaggcg
     1981 aatgtcgaac cggaacacgc ccgcacgccc cccgtcgggc acggccatga gggtgtgccg
     2041 gcgtggcgcg ggcaccagcg ccaggatcgg gtcactcccg ttcatgccct cgtccttgaa
     2101 atagatgcgc gtgaagagcc gcttcagcag accgcgcgcg aaaatgccga cgttgatgtg
     2161 cggtgcctgc aggctttcgc taggacccgg cacggcgccc ggcttgatcg tgctgaaggc
     2221 gaagccgccc cccgcatcgg tgggcgcgcg cgcaaagccg cgaaaggagt tggaccgcaa
     2281 ggggcggcca tcggcggggt gggcgtaatg gccctgcgcg tcggcctgcc agatttccag
     2341 gagggcatcg ggcagcccgt tgccctcgcc gtcgatcagg cggccttcga tgcgaatggg
     2401 cgtccccgtc acgccgggcg cgctcaagtc gggcccgaac aggggacgca cgttataggc
     2461 cgccggggcc aggcccaggt gcaggtaggg gccgacggtc tgcgaggggg tgcgctcagg
     2521 catggtcggg atccggcggg gtggcgttgg ggccgcgcag cacgatgtcg aaggtatagc
     2581 ccagcgccca ttcgggacgc gtcgtcgcca gatcgaactg ggataccatg cgtccgcgcg
     2641 ctgttgcgtc gctcaccgca ttgaaaatcg gatcgaaggg aaacaaggga tcgcccggaa
     2701 agtacatttg cgtgacaagc cgggtcagaa acgacggtcc gaacagggag aaatggatgt
     2761 gcgccggccg ccaggcattg gcatgattgc gccaggggta agcgccgggc ttgatggtga
     2821 tgaattcata aaccccgttg gcgtcggtca cgcagcgacc ggcgcccgtg aagttgggat
     2881 caagcggggc agggtgctgg tcgacgacgt ggatgtagcg accggcggca ttgcactgcc
     2941 agatctccac cagggtttcc ggcaccgg
//
LOCUS       DASTMT010000416         4911 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_5696481, whole genome shotgun sequence.
ACCESSION   DASTMT010000416 DASTMT010000000
VERSION     DASTMT010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4911)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4911)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4911
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5696481"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..449)
                     /locus_tag="VFR00_09750"
     CDS             complement(<1..449)
                     /locus_tag="VFR00_09750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010912154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAS-barrel domain-containing protein"
                     /protein_id="HEU0159589.1"
                     /translation="MDIRASEISSILKEQIKNFGKEAEVSEIGQVLSVGDGIARVYGL
                     DKVQAGEMVEFPGGIRGMALNLEADNVGIVIFGDDRSIKEGDTVKRTGAIVEVPVGKG
                     LLGRVVDALGNPIDGKGPIKADKRMQVDVKAPGILPRKSVNEPMATGL"
     gene            complement(449..1009)
                     /locus_tag="VFR00_09755"
     CDS             complement(449..1009)
                     /locus_tag="VFR00_09755"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013500298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HEU0159590.1"
                     /translation="MATDDPMMASMAGRYAAALFELAKEQKQLDQVARDVSAFQAMLE
                     GSPELRRLVRSPVIAAEGQARALDAILSRSGISGFTGNFLKLLVRNRRLFAVGDMLRV
                     FQALLARERGEVSADVASAHPLSAEQMQVLSDSLKASIGKQVQIRTRVDPNLLGGLIV
                     KVGSRMIDSSLRTKLNNLKVAMKGIG"
     gene            1338..1739
                     /locus_tag="VFR00_09760"
     CDS             1338..1739
                     /locus_tag="VFR00_09760"
                     /inference="COORDINATES: protein motif:HMM:NF019501.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BA14K family protein"
                     /protein_id="HEU0159591.1"
                     /translation="MLQWIRGPLAAALALAPLAAAAAAPPAGRLAPALEAANAGMHEV
                     QFRHHNGFGRGLGLGLGLGVLGAILASEAYGPPPGYDEDDYGYGGPPGAPADTAADPR
                     TLCARHFRSFEWNSGLYTTQSGERRLCPYLR"
     gene            complement(1816..4035)
                     /locus_tag="VFR00_09765"
     CDS             complement(1816..4035)
                     /locus_tag="VFR00_09765"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEU0159592.1"
                     /translation="MLARLEDGPASAGVGGPAVPVLLPVALDQTYDYLPPLDGEVRPG
                     AFVLVPFGAQTRIGLIWDRPPDAAGAAAANKKLKRITQILPDVPPLPSLSLRFAEWVA
                     RYTLTPLGMVARMMMGPGSLFEPARPRFGVRLAAAAPSRLSPARKRVLDAVADGQVRA
                     KSSLAAQAHCSTGVIDGLIAAGTLVEVAIPERRMPLPNPEHARVDFAGAQAQAVHAVG
                     ATASAEAFSVTLLDGVTGSGKTEVYFEAVAQTLRKGAQALIMLPEIALTSQFMDRFAR
                     RFGCMPVEWHSALSGSERARAWRASATGEARVVVGARSALFLPYRELGLIVVDEEHDQ
                     GFKQEDRVHYQARDMAIVRASLGKFGIILASATPSIESHVNARTARYRYVELPGRFSG
                     AKLPDIISIDMRKEPPAKGKWLASSLVDAVTETLAQGQQSLLFLNRRGYAPLTLCRSC
                     GHRLECPQCTAWLVEHRYRHRLNCHHCGFALPIPQNCPKCGEADCLVACGPGVERIAE
                     EVAERFPDARVALLSSDLVPSLVEMRQIIGTIESGAIDIVIGTQMVAKGHHFPRLATV
                     GVVDADLGLGTADPRAGERTFQLLHQVTGRAGRVLACGRGLVQTYLPEHPVMQAIISG
                     DRNQFLESEIRQRQTALLPPYGRLAALIVSARDKELAALVARELAHAAPSASRIEVLG
                     PAEAPLAVIRGRHRWRLLVKAPRELDIQGYLRLWLGNLRKLPSDVRLTVDIDPYSFL"
     gene            4147..4803
                     /gene="fsa"
                     /locus_tag="VFR00_09770"
     CDS             4147..4803
                     /gene="fsa"
                     /locus_tag="VFR00_09770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019963666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HEU0159593.1"
                     /translation="MKFFVDTADVTEIKELAATGLLDGVTTNPSLVAKAKRDFKEIIA
                     EICAAVAGPVSAEVAATDLAGMLREGRTLAKIAANVAVKVPLTWEGLKACRALTAEGS
                     MVNVTLCFSANQALLAAKAGATFISPFIGRLDDAGADGMDLVREIRTIYDNYPELQTQ
                     VLAASIRNVLHVKQAAMIGADAATMPPAILRALIAHPLTDRGLELFLADWKKTGQQIA
                     "
BASE COUNT          876 a         1622 c         1622 g          791 t
ORIGIN      
        1 aggccggtcg ccatcggctc gttcaccgac ttgcggggca ggatgccggg cgccttgacg
       61 tcgacctgca tgcgcttgtc ggccttgatc ggccccttgc cgtcgattgg gttaccgagc
      121 gcatcgacca cgcgcccgag caggcccttg ccgaccggca cctcgacgat ggccccggtg
      181 cgcttgaccg tgtcgccctc tttgatgctg cggtcgtcgc cgaagatcac gatccccacg
      241 ttgtcggctt cgaggttgag cgccatccca cgtatgccgc ccgggaactc gaccatctcg
      301 ccggcctgaa ccttgtcgag cccatagacg cgggcaatgc cgtccccgac cgacagaacc
      361 tgaccgattt cagagacctc ggcctctttg ccgaagttct tgatctgctc cttcaggatc
      421 gaggagatct ccgaagcgcg aatgtccatc agccgatacc tttcatcgca actttgagat
      481 tgttgagctt ggtgcggagc gaactgtcga tcatcctgct cccgaccttg acgatgagac
      541 cgccgagcag attgggatcg acgcgcgtgc gaatctgaac ctgcttgccg atcgaggcct
      601 tgaggctatc ggaaagcact tgcatctgct cggccgacag gggatgggcg gaggcaacgt
      661 cggcgctgac ctcgccacgc tcccgcgcca acagcgcctg gaacacccgt aacatatccc
      721 ccaccgcaaa cagccggcgg ttgcgcacga gcaacttgag gaagttgccc gtgaacccag
      781 aaatgccact tctgctgagg atggcatcga gcgcccgcgc ctggccctcg gcagcaatga
      841 ccgggctgcg caccagccgt ctcagctcgg ggctgccctc caacatcgct tgaaaggcgc
      901 tgacgtcgcg cgcgacctgg tcaagctgtt tttgctcttt ggcaagctca aacagggcgg
      961 cggcatagcg tcccgccatg ctcgccatca tgggatcatc ggtggccaag tgcgtggtct
     1021 cgtcccagaa ctttcagaga acaaccgtcg gctgaccgcg tccggcccgc cgctcgggcc
     1081 taaggccaaa tgccctcggt gcgcgtgcgc cccgcgcata gcatgggccg tgtttgccag
     1141 caaccggccg cgacggcatc gaatgccgca tcagcggcgg gaatgagcga cggagccggc
     1201 cgcaaaggcc gttatagcgc cagacaccga ccggggaccc tcgcgccgtc ggcaccttgg
     1261 caaagggccg agcctgcggg cgccagatct gcggggtgac aaattggggg gcagttggcc
     1321 cacaacagga gaacgtaatg ttgcagtgga tccgaggccc gctcgccgcg gcgctggctc
     1381 tggcacccct tgctgccgcc gcggcagccc cgccggccgg ccggcttgcg ccggcgctcg
     1441 aggcggcgaa tgcaggcatg cacgaggttc aattccgcca ccacaacggt tttgggcgcg
     1501 ggctcgggct cggccttggc ctcggcgtcc tgggcgcgat ccttgccagc gaggcctatg
     1561 gcccaccccc cggctacgac gaagacgatt atggctacgg agggccgccg ggcgcgcccg
     1621 ccgacacggc ggctgatcca cgcaccctgt gcgctcggca cttccgctcc ttcgaatgga
     1681 acagcggtct ttacacgacc cagtccggcg aaaggcgact ttgcccgtat ctgcgctagc
     1741 gcgagcaagt gagacgtgcg gtgcgggcgg ggtcatccag gcatggatgg cccctttttc
     1801 tcctcgcccg cttgctcaca aaaaactgta gggatcaatg tcgaccgtga ggcgcacgtc
     1861 gctcggcagc ttacgcaggt ttccgagcca caggcgcaaa tagccctgga tgtcgagctc
     1921 gcgcggggct ttgacgagca gacgccagcg gtggcggccg cgaatgacgg cgagcggcgc
     1981 ctcggccggg cccaatacct cgatgcggct cgcgctcggg gccgcgtgcg cgagctcgcg
     2041 ggcgacgagg gcggcgagct ccttgtcgcg cgcggagacg atgagggcag cgagacggcc
     2101 atagggcggc agcagcgcgg tttggcgctg gcgaatctcg ctttcgagga actgattgcg
     2161 gtcgcccgag ataatcgcct gcatcacagg gtgttcgggc aggtaggttt gcacgagacc
     2221 gcgtccgcag gcgagaacgc gcccagcacg ccccgttacc tgatgcaaca gctggaaggt
     2281 acgctccccc gcgcgtggat cggcggtgcc aagccccaga tccgcgtcga cgaccccgac
     2341 ggtcgccaag cgcgggaagt gatgcccctt ggccaccatt tgcgtgccga tcacgatatc
     2401 gatggcaccc gattcaatgg tgccgatgat ttggcgcatt tccacgagcg aaggcaccaa
     2461 atcggaagag agcagggcga ctcgcgcgtc gggaaagcgt tcggccacct cctcggcaat
     2521 gcgttcgacg ccagggccgc aggcaaccag gcaatcggcc tcgccacact tgggacagtt
     2581 ctgcgggatc ggcaaggcga agccacaatg gtggcagttg aggcgatgcc gatagcggtg
     2641 ttcgaccagc caggccgtgc actggggaca ctccagccgg tggccgcagg atcggcaaag
     2701 cgtcagcgga gcatagccgc gccgattgag gaagagcagg ctctgctgac cctgcgccaa
     2761 ggtctctgtc acggcatcga cgagggagct agccagccat ttgcccttgg cgggtggctc
     2821 cttgcgcatg tcgatgctga tgatgtcggg gagcttggcg ccggaaaacc gaccgggcag
     2881 ctcgacgtaa cggtaacgcg cggtgcgcgc gttgacgtgg ctttcgatcg agggtgtcgc
     2941 cgaggcgaga atgatgccaa atttgccaag gcttgctcgc acgatcgcca tatcgcgcgc
     3001 ctgataatgc acgcggtctt cctgcttgaa gccctggtcg tgctcctcat cgaccacgat
     3061 caaaccgagc tcgcgatagg gaagaaacag ggccgagcgc gcaccaacga cgacgcgggc
     3121 ctcgccggtc gcgctcgcgc gccaggcgcg ggcacgctcg ctgcccgaga gcgcggagtg
     3181 ccattcgacc ggcatgcaac cgaagcgccg ggcgaagcga tccatgaatt ggctcgtgag
     3241 cgcgatctcc ggcagcatga tgagagcctg ggctcccttc ctgagggtct gcgccaccgc
     3301 ctcgaaatag acctcggtct tgcccgaccc ggtgacaccg tcgaggagcg tcacggagaa
     3361 cgcctcggcg ctggctgtgg cgccgacggc gtgcacggcc tgggcctggg cacccgcgaa
     3421 atcaacgcgt gcatgctcgg ggttgggcag cggcatgcgc cgctcgggga tggcgacctc
     3481 caccagcgtg cccgccgcaa tcaagccgtc aatgacgccg gtggagcaat gggcttgcgc
     3541 tgccaagctc gatttggccc gcacctggcc gtcggcaacg gcgtcgagga cgcgcttgcg
     3601 cgccgggctc agacgcgaag gagcggcggc cgccaggcgc acgccaaagc gaggccgagc
     3661 gggctcgaac agggagccgg gccccatcat catgcgcgcg accatgccaa gcggcgtcag
     3721 cgtgtagcgc gcaacccact cggcaaaccg cagcgagagg ctcgggagcg ggggcacgtc
     3781 ggggaggatt tgcgtgatgc gtttcagttt cttgttggcc gcggccgcgc ctgccgcatc
     3841 gggcgggcga tcccagatca gcccgatgcg cgtttgcgcc ccgaacggca ccagcacaaa
     3901 ggcgcccggg cgcacctcgc catcgagggg cggcaggtag tcgtaggtct ggtcgagcgc
     3961 caccggcagc agcacgggaa cggccgggcc accgacgccc gcgcttgcgg ggccgtcctc
     4021 taagcgtgcg agcagacttt ccaaaccaac tctcgcggac tagaacgccg aatcaggttc
     4081 tgagcagtga ggcactttag cggcccctgc ggcaagccca aggcaactgg ggagcacctg
     4141 cgcgccgtga aattcttcgt cgataccgcg gatgtgactg agatcaagga gttggccgcg
     4201 accggtctgc tcgacggggt caccaccaac ccctcgttgg tcgccaaggc caagcgcgac
     4261 tttaaggaaa tcatcgccga aatttgtgct gccgtggccg gccccgtcag cgctgaggtg
     4321 gccgccaccg accttgccgg gatgctgcgc gagggccgta cgctggccaa gatcgccgcc
     4381 aacgttgcgg tgaaggtccc gctcacctgg gagggcctga aggcctgccg ggccctcacg
     4441 gccgagggca gcatggtcaa cgtgaccttg tgcttttcgg ccaatcaggc gctgctggcg
     4501 gccaaggcgg gggcaacctt catttcaccg ttcatcggcc gcttggatga tgcgggagcc
     4561 gacggcatgg acctcgtgcg cgaaatcagg accatctacg acaactaccc ggaattacag
     4621 acccaggtgc tggcggcctc aattcgcaat gtcttgcacg tcaagcaggc cgccatgatc
     4681 ggcgccgatg ccgcgaccat gccgccggcc attctgcgcg cgctgatcgc gcatccctta
     4741 accgaccggg ggctcgaact gttcttggcg gattggaaaa aaaccggcca acaaatcgcc
     4801 tgaaaacctt caccggcacc ctgtcagggg cgctcggcga gctgtccctc ggagaccacg
     4861 cgctcgaagg cttccgccgt gcacttaatg cggtagagca gatcccgacc a
//
LOCUS       DASTMT010000417         5000 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_231942, whole genome shotgun sequence.
ACCESSION   DASTMT010000417 DASTMT010000000
VERSION     DASTMT010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 5000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5000
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_231942"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..188
                     /locus_tag="VFR00_09775"
     rRNA            <1..188
                     /locus_tag="VFR00_09775"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            328..442
                     /gene="rrf"
                     /locus_tag="VFR00_09780"
     rRNA            328..442
                     /gene="rrf"
                     /locus_tag="VFR00_09780"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(549..1391)
                     /locus_tag="VFR00_09785"
     CDS             complement(549..1391)
                     /locus_tag="VFR00_09785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEU0159594.1"
                     /translation="MSAFDAAWLDLREPVDHRSRNRVLAQALARHFDGWRPLTVIDLG
                     CGTGSNLRAAAPFLGPEQHWTLVDLDAALLAAASTRLTAWADAADDSHGTLVLFKGQK
                     RISVEFRRADLAGDLDAALGPTAHLVTAAALFDLVSAPFIERFATALAARRSAFYTVL
                     TYDGDQRWTPPHPADAAMLEAFHRHQSRDKGFGAAAGPGAPDHLSESFAHLSYTVQEA
                     DSAWRLGPQDAGLIADLARGFASAVEETGAIDRGTLARWREVQRTGAVVGHTDTLALP
                     SLRA"
     gene            complement(1388..2434)
                     /locus_tag="VFR00_09790"
     CDS             complement(1388..2434)
                     /locus_tag="VFR00_09790"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018259558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEU0159595.1"
                     /translation="MITAGFAIPGDLAQPTGGYVYARRVLERFPLAEVRLQHVALPAS
                     FPAAGPDDIAESVRRLQALSADTIVLFDGLAYGAMPGDAIAGLKQRIVALVHHPLGLE
                     AGLPAARQRQLLALETVALGLARRVLVTSRATARTLVSDFAVPAAKIAIAEPGTLPAP
                     RARGTGAPAQLLAVGSIVPRKAYPVLVRALRPLVGLAWQLTIVGATDRSPSALAELNA
                     ALAETGLAARITILGPLAETELAGHYDRADLFVLPSLYEGYGMVLAEAMARGLPIVCT
                     TGGAAAETVPDGAALKVAPGDAAALSAALGRALAEVSLRRRLAEASWAAGRALPGWEE
                     TARIIAHVIRESAA"
     gene            complement(2505..2903)
                     /locus_tag="VFR00_09795"
     CDS             complement(2505..2903)
                     /locus_tag="VFR00_09795"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012334501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HEU0159596.1"
                     /translation="MYAVEVRDRIMIAHSLPDPFFGPAQGLHGATFVVDVAFYRENLT
                     PQNVVVDIGAALEVLHKTLKPLAYQNLDSLPQFSGRLTTTEFLCRHIFEAMAGAAREG
                     ALGADGKELAKIKVTLHETDLARASFEGTP"
     gene            complement(2921..3925)
                     /locus_tag="VFR00_09800"
     CDS             complement(2921..3925)
                     /locus_tag="VFR00_09800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HEU0159597.1"
                     /translation="MMARRATGKTRMARALWYVQKGRVELRAAPLPPPAAGEARVRSL
                     FSGISRGTERLVLDGAIGPSEWERMRAPMQEGAFPFPVKYGYAATGIVEAGPANLLGR
                     QVFALHPHQDFFNAPLQALAPIPESVPARRATLAANMETALNAIWDGGAGPGDRIVIV
                     GAGVLGLLLASLAARLPGAIVTAVDAEESRRALAEGLGIGFAKPEHAPPDADIVFHAS
                     ATGPGLNTAIRCAGLEAPIVELSWYGDRAASVELGAAFHSRRLRILCSQVGQIAPSRR
                     PRWSFKRRLEAALGLLTVPALDALVAEEMAFETAARGLPKALASTAAGLAPVIRYPEA
                     "
     gene            3986..>5000
                     /locus_tag="VFR00_09805"
     CDS             3986..>5000
                     /locus_tag="VFR00_09805"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013948211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU0159598.1"
                     /translation="MSDSLDAPAPGALPPPALSLTTPTGLQSIDELTRRRRLVLGLNL
                     ITYALMLGWASRILAAGGWTWIDAVLFLAFALGTPWAVLGFWNALIGLWLLNFGGKDA
                     RMAVAPFAAAGDCPTPIRIKTAVFMTLRNEDATRAIERFKIVADSIERTGEGGAFSYF
                     VLSDSNDASVGAAEERAVAAWQAAAGNPERIIYRRRSNNVGYKAGNVRDFCARWGDQF
                     GLMLPLDADSLMAGEEIVRLVRIMQAHPKIGILQSLVVGMPSQSAFARIFQFGMRHGM
                     RAYTMGQAWWVGECGPFWGHNALVRVQPFYEHCDLPVLPGTPPLGGHVLSHDQVEATL
                     MRRA"
BASE COUNT          814 a         1695 c         1720 g          771 t
ORIGIN      
        1 gcgccagcgg caccgcaggg tagctatgtg cggacgggat aaccgctgaa agcatctaag
       61 cgggaaaccc acctcgaaac gagtactccc ttgagagccg tggaagacga ccacgttgat
      121 aggccgggtg tggaagcgcc gtaaggcgtg tagcttaccg gtactaatag ctcgatcgaa
      181 cttgaacgct ctcattaagc aatgctcatc cggtcttgtc ggccctgccg gcaagacggc
      241 cgaccctcac gggctcgtgg ggacggccgc gatgagcgtg agctcacctc tagaaacaca
      301 agaccagttt gctcaccctt ttgccgacct ggtgattata gcggggtggc tgcacccgat
      361 cccattccga actcggccgt gaaacgcccc tgcgccgatg gtactccgtc tcaagacgcg
      421 ggagagtagg tcgttgccag gtctgccaag gggtgaatgc aaaacgcatc gagatccctt
      481 cttcatgttc ttttggaaaa aggccgtcgc ttcgccgcga cggccttttt gtcatccagc
      541 tacgacgctt aagcccgcaa cgacggcaat gccagcgtgt cggtatgccc gaccacggcg
      601 cccgtgcgtt gaacctcgcg ccagcgcgcc agcgtgcctc gatcgatcgc cccggtctct
      661 tccacggcgc tcgcaaatcc ccgcgccaaa tcagcaatca gcccggcgtc ctgcggccca
      721 agccgccagg cgctgtcagc ctcctgcacc gtgtagctca ggtgagcaaa actctcgctc
      781 aaatggtccg gcgcgcccgg accggcggcg gcgccaaatc ccttgtcgcg gctttggtgg
      841 cggtggaagg cctcgagcat ggcggcgtcg gcgggatgcg gcggcgtcca gcgttgatcg
      901 ccatcgtagg tcaggaccgt gtagaaggcg cttcggcgtg ccgccagggc ggtggcgaag
      961 cgctcgatga acggcgccga gacgaggtcg aacagggcgg ccgccgtcac caggtgggcg
     1021 gtcgggccga gcgccgcgtc caagtcgccg gcaaggtcgg cgcggcggaa ctcgacgcta
     1081 atgcgttttt gccccttgaa cagcacgagc gtgccgtggc tgtcgtcggc ggcgtcggcc
     1141 caggcggtga gccgcgtcga ggccgccgca agcagcgccg cgtcgagatc cacgagcgtc
     1201 cagtgctgct cgggacccag gaacggggcg gcggcgcgca ggttcgagcc cgtgccgcag
     1261 cccaaatcga tgacggtcag cgggcgccag ccgtcgaagt gccgggccaa ggcctgagcc
     1321 aagacccggt tgcgcgagcg atggtccacc ggctccctga ggtcgagcca ggcggcgtca
     1381 aacgcgctca tgccgcgctc tccctaatga cgtgtgcaat gatgcgcgcg gtctcctccc
     1441 agccgggcaa ggcccggccc gcggcccagg atgcctcggc caatctccgg cgcaaggaaa
     1501 cctccgccaa ggcgcgcccg agcgcggccg acagggcggc cgcgtcgccc ggcgccacct
     1561 taagcgcggc gccgtcgggg accgtctcgg ccgccgcgcc gcccgtggtg cacacgatcg
     1621 gcaagccccg ggccatcgcc tcggccagca ccatgccata gccctcgtag agcgacggca
     1681 gcacgaagag atcggcgcga tcatagtgac cggcgagctc ggtctccgcg agcggaccca
     1741 agatcgtgat ccgggctgcg agcccggttt cggcgagcgc cgcgttgagc tccgccagcg
     1801 ccgaggggct gcgatcggtc gcacccacga tggtgagctg ccaagctagc cccaccagcg
     1861 gtcgtaatgc ccgcaccagg acagggtagg ccttgcgcgg cacgatcgag ccaaccgcga
     1921 gcagctgcgc tggggcgccg gtgccgcggg cgcggggcgc cggcaaggtg cccggctcgg
     1981 caatggcgat ctttgccgcc ggcacggcga aatcgctaac gagcgtgcgc gccgtggcgc
     2041 ggctcgtcac cagcacgcgc cgggcgagcc cgagcgcgac cgtttccagg gccaggagct
     2101 gccgctggcg cgccgccggc agcccagcct ccaggcccaa gggatgatgc acgagggcaa
     2161 cgatgcgctg cttgaggccg gcaatggcat cccccggcat ggcgccatag gccaagccgt
     2221 cgaacagaac gatcgtgtcg gctgagagcg cctgcaaacg gcgcacgctt tcggcgatgt
     2281 cgtcgggccc cgccgcgggg aaggatgcgg gcagcgccac gtgctgcagc cgaacctcgg
     2341 ccaagggaaa gcgctccagc acgcggcgcg cgtagacata gcccccggtt ggctgcgcga
     2401 ggtcaccggg aatggcgaag cctgcggtga tcatggtgcc ccgccggcca cggcggcgcc
     2461 aaagcgcacc ggcggcagcg tgcggcgatg gacccggcgt gggttcacgg cgtcccctcg
     2521 aaggaggccc gcgccagatc ggtttcgtgc aacgtgactt tgatcttggc gagctccttg
     2581 ccgtccgcac ccagggcccc ctcccgtgcc gcgcctgcca tcgcctcgaa aatatggcgg
     2641 cagaggaact ccgtggtggt cagcctgccg ctaaattgcg gcaggctgtc gaggttctga
     2701 taggccaagg gtttcagggt cttatgcagc acctccagcg ccgcgccgat gtcgacgaca
     2761 acgttctgag gggtgaggtt ttcgcggtag aaggcgacat cgaccacgaa ggtggccccg
     2821 tgcaggccct gggccggacc aaaaaacgga tcgggcaagc tgtgcgcgat catgatgcgg
     2881 tcgcgcacct cgacggcgta catggcatct ctcctcctga tcaggcttcg ggatagcgga
     2941 tgacgggggc gagccccgca gcggtgctgg ccagcgcctt gggcaggccg cgggcggcgg
     3001 tctcgaaggc catctcttcg gcgaccaggg cgtcgagagc gggcactgtc aacaatccga
     3061 gcgcggcctc cagccggcgc ttgaagctcc agcgtggccg ccggctgggc gcgatctggc
     3121 ccacttgcga gcacaggatc ctgaggcggc ggctgtgaaa ggcggcgcca agctcgacgc
     3181 tggcggcccg gtcgccgtac cagctgagct cgacaatggg ggcttcgagg cccgcacagc
     3241 ggatggccgt attcagaccc gggccggtgg cgctggcatg aaagacgata tcggcatcgg
     3301 gcggcgcgtg ctccggtttg gcaaaaccga tgcccaggcc ttccgccagc gccctgcggc
     3361 tctcctccgc gtcgaccgct gtcacgatgg cgccaggcaa acgggcagca agcgaggcca
     3421 acaggagccc gagaacgccg gcgccgacga tgacgatgcg atcccccggg cccgcgccgc
     3481 catcccagat ggcgttgagc gcggtttcca tgttggcggc caaggtggcg cgccgcgccg
     3541 gcacgctctc aggaatgggc gccagcgcct gcagcggggc gttgaaaaag tcctggtgcg
     3601 gatgcagggc aaacacctgc cggcccagca gattggccgg tccggcctcg acgatgccgg
     3661 tcgcggcgta gccgtatttg accgggaacg gaaaggcacc ctcctgcatg ggtgcgcgca
     3721 tgcgctccca ttcgctcggg ccgatggcgc cgtcgaggac caggcgctcc gtgccgcggc
     3781 tgatgccgct aaacaggctg cgcacgcggg cttcgcccgc tgccggcggc ggcaaggggg
     3841 ccgcgcgcag ctcgacgcgg cccttctgca cgtaccagag ggcccgcgcc atgcgggtct
     3901 tgcccgttgc ccggcgcgcc atcatgttac cgctccgaat tgccaggatt tgaccgctgc
     3961 cataaaccac ccggatgaac ccttgatgag cgacagcctc gatgcccccg ccccgggggc
     4021 actgcccccg cccgcgctga gccttacgac acccaccggc cttcagtcca tcgatgagct
     4081 gacgcgccgc cgccgcctcg tgctcggcct caacctcatc acctatgccc tgatgttggg
     4141 atgggccagc cgcattctcg ccgcgggcgg ctggacctgg atcgacgccg tcctgttcct
     4201 ggcctttgcg ctcggcacgc cttgggccgt gctcggcttt tggaacgcgc tgatcggcct
     4261 gtggctgctc aactttggcg gcaaggatgc gcgcatggcc gtggcgccgt tcgccgcggc
     4321 gggcgattgc ccgacgccga tccgcatcaa gaccgcggtg ttcatgacgc tgcgcaacga
     4381 ggacgccacc cgcgccatcg agcgcttcaa gatcgttgcc gacagcatcg agcgcacggg
     4441 agagggtggt gcattttcct acttcgtttt aagcgacagc aacgatgcct cggtcggtgc
     4501 cgccgaggaa cgcgctgttg ctgcctggca ggcggccgcc ggtaatcccg aacgcatcat
     4561 ctatcgccgg cgcagcaata atgtcggcta caaggccggt aacgtgcgcg acttctgcgc
     4621 gcgctggggc gatcagtttg ggctgatgtt gccgctcgat gcggacagcc tgatggccgg
     4681 cgaggagatc gtgcgcttgg tgcgcatcat gcaggctcac ccgaaaatcg gcattctgca
     4741 gagcctggtg gtgggcatgc cgtcgcagag tgcttttgcc cgcatcttcc agttcggcat
     4801 gcgccacggt atgcgcgcct acaccatggg tcaggcctgg tgggtcgggg agtgcggccc
     4861 cttctgggga cacaacgcgc tggtgcgggt gcagccgttc tacgagcact gcgacctgcc
     4921 cgttctgccg ggaacgccgc cgcttggcgg ccacgtcttg tcgcacgatc aggtggaggc
     4981 gaccttgatg cgccgcgccg
//
LOCUS       DASTMT010000418         2389 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_2857157, whole genome shotgun sequence.
ACCESSION   DASTMT010000418 DASTMT010000000
VERSION     DASTMT010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2389)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2389)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2389
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2857157"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..543)
                     /locus_tag="VFR00_09810"
     CDS             complement(<1..543)
                     /locus_tag="VFR00_09810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HEU0159599.1"
                     /translation="MSGGRRISEAELHAFVDGELLDKEAAEVEAALATAAEDLRVARS
                     IAALNDRLGQHYAHVLDEPIPARMAALAARAGRRAGGWAPLRLGRALGIAVLLLAGAL
                     AGYAAAIWGPGEAGRQPAFVANALSAHQVFVPEVRHPVEVGAAEEKHLLQWLTRRLGT
                     QVRAPELGGQGWKLVGGRLLP"
     gene            complement(540..1064)
                     /locus_tag="VFR00_09815"
     CDS             complement(540..1064)
                     /locus_tag="VFR00_09815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011501733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEU0159600.1"
                     /translation="MRVTSRRDAVAAEIPRLRRYARALVGDSSEADDLIQDTLERALA
                     RLGQWRDGDNPRKWLFSILHNLHVDGLRRSSRRPPHVGLDSLGPDQSAPAADGASGGD
                     LDRALRLLTPDQREVVLLVGLEGLSYAETAEVLSIPIGTVMSRLARGRDRLRTLLAYG
                     LENEAEHGGLRRVK"
     gene            1298..1741
                     /locus_tag="VFR00_09820"
     CDS             1298..1741
                     /locus_tag="VFR00_09820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018391381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEU0159601.1"
                     /translation="MLRVVAAAAALAIGATVVFAQNAAVIDQRKQAMKAFGGAAKSGG
                     TMAKGDAPFDLAKAQESLKTIQTKAAEAKNLFPDDSKTGETDALPEAFANKADLMAKF
                     DKLAADAKAAEAAIKDEASFKAEWPKVTSNCGGCHKAYRKPPPQK"
     gene            1881..>2389
                     /locus_tag="VFR00_09825"
     CDS             1881..>2389
                     /locus_tag="VFR00_09825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011502047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEU0159602.1"
                     /translation="MRLSRIIMVLALLAVAAAGLFYVLTMPKPAVTGTLPSRTANLAN
                     GEVMFNAGGCASCHATPKQEDTRRLGGGLALNTAFGRFYVPNLSSDPAHGIGAWSEEA
                     FVNAMLRGIGRDGEHLYPAFPYTSYQRMRLDDVRDLFAFLRTLPPDATPSRPHELPLP
                     FNLRRGLGL"
BASE COUNT          411 a          850 c          754 g          374 t
ORIGIN      
        1 gggcagcaac cggccgccga cgagcttcca gccctgccca ccgagttcgg gtgcccgcac
       61 ttgcgtgcca aggcggcgcg tcaaccactg cagcagatgc ttttcctccg ccgcaccgac
      121 ctcgacggga tggcgcactt cgggcacgaa cacctggtgg gcgctcaaag cgttggccac
      181 gaaggctggc tgacgcccgg cctctccggg cccccagatt gccgccgcgt aaccggcaag
      241 ggcaccggcc agcagcagca cggcgatgcc cagcgcccgg cccagacgca gcggcgccca
      301 gcccccggca cggcggccgg cgcgcgccgc cagcgccgcc atcctggcgg ggatgggctc
      361 atcgagaacg tgggcgtagt gctgaccgag acggtcgttg agcgcggcga tggagcgcgc
      421 cacgcgcagg tcctcggctg ccgttgccag cgccgcctcg acctcggcag cctccttgtc
      481 cagcaattcg ccgtccacga aggcgtgcag ttcggcctcg ctgatgcgcc gcccgccgct
      541 cacttgaccc tccgcaggcc gccatgctcg gcttcgtttt caaggccata ggccagcaag
      601 gtgcgcagcc ggtcgcgtcc acgcgccagt cgggacatca cggttcctat cgggatcgac
      661 agcacctcgg ccgtttccgc gtaactgagg ccttccaggc ccaccagcag caccacctcg
      721 cgctgatcgg gcgtcaataa gcgcagcgcc cgatcgagat cgcctccgct tgccccatcc
      781 gccgccggcg ccgactggtc gggaccgagg ctgtcgaggc cgacgtgggg cggccggcgc
      841 gagctgcggc gcaggccgtc cacgtgcaga ttatgcagta tagaaaacaa ccacttgcgc
      901 ggattgtctc cgtcccgcca ttgccccaga cgcgccagcg cgcgctcgag cgtgtcctgg
      961 atcagatcat cggcctcgct gctgtcgccg accagggccc gcgcgtaacg ccgcaagcgt
     1021 ggaatttctg ccgcaacggc atcccggcga gaggtcacgc gcacgtcctt tcctactggc
     1081 cggcacacag taggacttat gccagcagac gacggtggcc tccaatttat tccgcgcgac
     1141 gcatcaaaat aatgtgcggc gcgaggcgtt gaaaccgcgc ctcgcctcgt taaagttggt
     1201 gcctgcacga atccagcgtc gtgcgcaacg ggcgggccgc ggagagtcct cgagggttga
     1261 ggttgccgcg ccggcaattt ctcgggagga atctcatatg ctacgcgtcg tcgccgctgc
     1321 tgcagccctc gccatcgggg caacagtggt gtttgcccag aatgctgccg tcatcgacca
     1381 acgcaaacag gccatgaagg cgtttggcgg agcggccaag agcggcggga ctatggccaa
     1441 gggtgatgcc cccttcgatc tcgccaaggc gcaggagtcg ctcaagacca ttcagaccaa
     1501 ggccgccgaa gccaagaacc tgtttcccga tgactccaag acgggcgaaa ctgacgcctt
     1561 gcctgaggcg ttcgccaaca aggccgacct gatggcgaag ttcgacaaac tcgccgccga
     1621 cgccaaggcc gccgaggccg cgatcaagga cgaagcgagc ttcaaggccg aatggccgaa
     1681 agtgacgagc aattgcggcg gctgccataa ggcctaccgc aaaccgccgc cgcaaaagta
     1741 gggcgcggac cgccagtggt ctttcagcgt tcggaagccg ccggcggcag ggatccaaga
     1801 caaaagcgcg cgagccggtc tcgcgcgctt ttgtgcatca tggtggcgct cggcaggacg
     1861 gcgccgcgca gggaggccct atgcgcctga gccggatcat catggtgctg gcgctgctgg
     1921 ccgttgccgc tgccggcctg ttctatgtcc tcaccatgcc gaagccggcc gtgaccggca
     1981 cactaccgtc ccgcaccgcc aatctcgcca acggcgaggt catgttcaac gccggggggt
     2041 gcgcctcctg tcacgcgacc cccaagcagg aagacacccg acggctcggc ggcgggctcg
     2101 ccctcaacac cgcctttggc cgcttttatg tgcccaacct gtcatcggat ccggcgcatg
     2161 gcatcggcgc ctggtcggaa gaagccttcg tcaatgccat gctgcgcggc atcggtcgcg
     2221 acggcgaaca cctctacccg gcttttccct atacctccta tcagcgcatg cggctcgacg
     2281 acgtgcgcga cctatttgcc tttttgcgca cgctgccgcc cgacgccaca ccgagccggc
     2341 cacacgagtt gccgttgccc ttcaacctgc gccgcgggct tggcctgtg
//
LOCUS       DASTMT010000419         2503 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_3188271, whole genome shotgun sequence.
ACCESSION   DASTMT010000419 DASTMT010000000
VERSION     DASTMT010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2503
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3188271"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            493..1740
                     /gene="dinB"
                     /locus_tag="VFR00_09830"
     CDS             493..1740
                     /gene="dinB"
                     /locus_tag="VFR00_09830"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019859373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEU0159603.1"
                     /translation="MAGAATILHADLDAFYASVEQLLNPALRGKPIAVGGGVVLAASY
                     EAKAFGVRGGMPGRRALELCPHLRFVEGHFDLYQRLGDAALKVVSDYTPLVERLSIDE
                     AFADVAGCERLFGSPLEIAKTIRRRVREELGLPISVGIARTKHLAKIASQVAKPDGLV
                     LVDPASELAFLHDLPVHLLWGVGPATEARLAEIGVITIGQLAHLPDARLSGLLGRALG
                     TKLTALAWNCDPRLVRTAARAQSAGAQCALGHQLAHPSLTRPVLWQLADRIGTRLRAK
                     RRPGWTISVRVRFADLRAVSRSMTLPQPISATAMLAEIAEDLVRAVLAAHPEERTISL
                     LAISVSRLEPHPVTQLELPLGLADEAHRPGSQKGGARARADRAMDKVRDRFGWAALRY
                     GPLAMGQLRSVPDAFRTLAEKEL"
     gene            complement(1772..>2503)
                     /locus_tag="VFR00_09835"
     CDS             complement(1772..>2503)
                     /locus_tag="VFR00_09835"
                     /inference="COORDINATES: protein motif:HMM:NF042648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N,N-dimethylformamidase beta subunit family
                     domain-containing protein"
                     /protein_id="HEU0159604.1"
                     /translation="HTRATLDALKAYRDGGGRFCYLGGNGFYWKIALAEQQPGLLEIR
                     RAEGGIRAWAAEAGEYYNQLDGEYGGLWRRNGRPPQELVGVGFTAQGAFDGSYYRLKP
                     GVRQDARVAWMFTGIEEDIIGDFGLSAHGAAGFELDRTDKRLGTPLQAVVVAASENHP
                     PETPWVLVPEEYLTHLSTTTGQPPAEMIRADMTFFATAQGKGAVFSTGSITFCGSLPS
                     HGYDNNISRLLGNVLERFLDPRPFN"
BASE COUNT          396 a          839 c          838 g          430 t
ORIGIN      
        1 cgacgcgaac acccttgagg cactcgagtt cgatgccaat gctcatcgcc actctcccgt
       61 tgcggccaat catttatgct tagcatcgcc tagccgccca caccatcgcg aggcctcctc
      121 ggcagaacga gcgcgcgtaa gcaaggtccg gcacaaacgc gttgcaaaag cggcgagggc
      181 cggcggacac gtcgtgcatc cacgctttgg ccgcgcggcg gccctgcctc gtcacgtggc
      241 caggatccgt tggcacccgt tcctcgcagg cacggacaac cttctgcgcg cgccaggacc
      301 ttggtcaccg cggcgaggcc cggtgcggcc cccgcgctcg accagaaaag gacccaacag
      361 ggttcgcccg ggcctgcacc cctagtctct tgacagcggg cctcttgttc ccgttatgtt
      421 ctcaaaggtc acggcgtatt tggccccgcg cctgcgcgaa ctcgggcaag ggatgcaagg
      481 ggatgccttg atatggcagg tgccgccacc atcctgcatg cggatctcga cgccttctat
      541 gcctccgtcg aacagctcct caatcccgcc ttgcgcggca agcccattgc ggtcggtggc
      601 ggcgtggtgc ttgccgcctc ctatgaggcc aaggccttcg gcgtgcgggg cggcatgccg
      661 gggcggcggg cgcttgagct ctgtccgcac ctgcgcttcg tggaggggca tttcgatctc
      721 tatcagcgct tgggggatgc cgcgctcaag gtggtgagcg attatacgcc gctcgtcgag
      781 cggctttcga tcgacgaagc cttcgccgac gtggcgggat gcgagcgtct gttcggatcg
      841 ccgctggaga ttgccaaaac gatccgccgg cgcgtgcgcg aggaattggg actgcccatc
      901 tcggtgggga tcgcgcgcac caagcatctc gccaaaatcg cctcgcaggt ggccaagccc
      961 gatggtctcg tcctggtcga cccggcatcc gagctggcgt tcctgcacga cctacccgtg
     1021 catttgctgt ggggcgttgg cccggcgacg gaggcccggc tcgccgagat cggcgtcatc
     1081 accataggtc agttggcgca tctgccggac gcgcggctga gtggcctcct gggccgagcg
     1141 ctcggcacga agttgaccgc gctcgcatgg aactgcgatc cgcggctggt taggacggca
     1201 gcccgggcgc agtcggcggg agcgcagtgc gcgcttggcc accagctggc gcatccaagc
     1261 ctcacgcggc cggtgctttg gcagcttgcg gaccggatcg ggacgcgctt gcgggccaag
     1321 cgccggccgg gctggaccat atccgtacgc gtgcggttcg ccgacctgcg cgccgtcagc
     1381 cgctccatga ccttgccgca gcccatttcg gccacggcca tgctggccga aatcgccgaa
     1441 gacctggtgc gggccgtgct ggccgcgcac cccgaggagc ggacgatctc gctgctcgct
     1501 atttccgtgt cgaggctcga gccacacccc gtgacccaac tcgagctgcc gctcgggctg
     1561 gcagacgagg cgcatcgtcc cggttcgcaa aaaggtgggg cacgcgcgcg tgccgacagg
     1621 gcgatggata aggttcgtga tcgcttcggc tgggcggcgc tgcgctacgg gccgcttgcc
     1681 atggggcagc tacgctccgt gcccgacgcg ttccgcacgc tcgccgaaaa agaactctga
     1741 gcgggcctcc gcgccacggg gcgcccctgc ctcagttgaa gggccgcgga tcaaggaagc
     1801 gctcgagcac gttgcccaat aagcgggaga tattgttgtc gtagccgtgg ctcggcagcg
     1861 aaccgcaaaa ggtaattgat ccggtcgaga acacggcacc tttaccctgg gcggtcgcaa
     1921 aaaacgtcat gtcggcgcgg atcatttcgg cggggggctg gccggttgtg gtcgagaggt
     1981 gggtcaagta ctcctcgggt accagcaccc agggggtctc gggtgggtgg ttctcgctgg
     2041 ccgcgaccac cacggcctgg agcggggtgc ccaggcgctt gtcggtgcga tcgagttcga
     2101 agcccgccgc accgtgcgcg gacaagccga agtccccgat gatgtcctcc tcgatgccgg
     2161 tgaacatcca ggcgacgcgc gcgtcctggc gcacgcccgg cttgagccga taataggagc
     2221 cgtcgaatgc accctgggcg gtgaagccca cgccgaccag ttcctgcggc ggacggccgt
     2281 tgcgccgcca cagcccacca tactcgccat cgagctggtt ataatattcg cccgcttccg
     2341 ccgcccaggc gcgaatgccg ccctcggcgc ggcggatttc gagtagcccg ggctgctgtt
     2401 ctgccaaggc gatcttccaa taaaagccat tgccgccgag atagcagaag cgtccgccgc
     2461 catcgcgata ggccttgagg gcatcgaggg ttgcccgcgt atg
//
LOCUS       DASTMT010000420         2074 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_1875661, whole genome shotgun sequence.
ACCESSION   DASTMT010000420 DASTMT010000000
VERSION     DASTMT010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2074
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1875661"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..502)
                     /locus_tag="VFR00_09840"
     CDS             complement(<1..502)
                     /locus_tag="VFR00_09840"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEU0159605.1"
                     /translation="MIFGLTLPATRLAVADFDPLFVTLGRSLVAAALAGLTLLWARPQ
                     APARRDWPRLAAFALFSIVGFPLLMAIAMQHAPASHGAVVLAVLPLLTALAGALIAGE
                     RPSVGFWACGVAGTVSVLCYSVVTGSASDELQWADLLLAVSAMSAAMAYALGGEMAGR
                     IGGWEVI"
     gene            complement(589..1737)
                     /locus_tag="VFR00_09845"
     CDS             complement(589..1737)
                     /locus_tag="VFR00_09845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011315381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEU0159606.1"
                     /translation="MDFSLNEEQAAIRETALAFAQERIAPFAAEWDEKCHFPVDVIRE
                     LAALGMATIYVPEAKGGAGLSRLDGALIMEALAYGCPAISGYVSIHNMVAWMVASYAS
                     EAQVAAWMPKLASMQWLSSSCLTEPGAGSDAAALKTSARLKGDHYLLNGSKQFISGAG
                     ASDFYFVFARTSEEGAHGISAFAVMKDTPGLSFGPNEKKMGWNAQPTRQVMFDNCKVP
                     VANLIGEPGRGFRFAMSGLDGGRINIAACSLGAAWLALDKARGYMRERGAFGRPLADF
                     QALQFKIADMATELEAARLLTYRAAEALDRADPQASMYSAMAKRLATDLGFQVCNEAL
                     QIHGGYGYLKDYGVEKLVRDLRVHQILEGTNEIMRVVISRKLLGGNRG"
BASE COUNT          411 a          696 c          657 g          310 t
ORIGIN      
        1 aaatgacctc ccagccgccg atgcgcccag ccatttcgcc gcctagggcg taggccatgg
       61 ccgccgacat ggccgagacc gccaacaaga ggtcggccca ctgcagctcg tctgacgcgc
      121 tcccggtcac caccgagtaa cacaggacac tgacggtccc cgccacaccg caggcccaaa
      181 atcccacgga ggggcgctcg ccggcgatga gcgcaccagc cagcgccgtc aacagcggca
      241 gcaccgccaa caccacggcg ccgtggctgg ccggcgcatg ctgcatggca atcgccatca
      301 gcaagggaaa gccgacgatg gagaacaggg caaaggcggc aagccgcggc cagtcgcggc
      361 gcgccggcgc ttgcggccgc gcccacagca gggtaaggcc ggccagagcg gcggccacca
      421 gcgagcggcc gagcgtcacg aacaaaggat cgaaatcggc gacggccagc cgtgttgccg
      481 gcaaggtgag cccgaagatc atgaccccga gcacgccaag caggagggct tggcgctcac
      541 ggctccgtgg ccggccggtc gctgccgcct ttgagccgag ctgtcctctc acccgcggtt
      601 gccgcccaag agcttgcgcg aaatcaccac gcgcatgatc tcgttggtac cctccagaat
      661 ttggtgcacc ctgagatcgc gcacgagctt ttcaacgccg tagtctttga gatagccgta
      721 gccgccgtgg atttgcaggg cctcgttgca cacctggaag ccgaggtccg tggcaaggcg
      781 cttggccatc gccgaataca tggacgcctg cggatcggca cgatcgagcg cttcggcggc
      841 acgataagtc agcaatcgcg ccgcctccag ttccgttgcc atatcggcga tcttgaactg
      901 cagcgcttga aagtcggcga gcggtcgccc gaaggcgccg cgctcgcgca tgtagccgcg
      961 agctttgtcc aaggcgagcc aggccgcacc cagcgagcag gcagcgatgt tgatgcgccc
     1021 gccgtcgagc ccgctcatgg caaagcgaaa accgcgtccc ggctcgccga tcaggttggc
     1081 gaccggcacc ttgcagttgt cgaacatcac ctgtcgggtt ggctgcgcgt tccagcccat
     1141 ttttttctcg ttggggccaa aggaaagccc cggggtatcc ttcatcaccg cgaaggctga
     1201 gatgccgtgg gcgccttcct cgctcgtgcg agcaaagacg aaataaaaat cgctcgcacc
     1261 ggcgccggag atgaactgct tgctgccatt gagcagatag tgatcaccct taagcctggc
     1321 cgaggtcttc agggcggcgg catcggagcc cgcaccgggc tcggtcaggc aagagctgga
     1381 tagccattgc atcgaggcaa gcttgggcat ccaggcggcg acctgagcct cggaggcata
     1441 actggcaacc atccaggcca ccatattatg aatggagacg tagccggaaa tggcgggaca
     1501 gccataggcc agggcctcca tgatcagcgc gccatcgaga cgggaaagcc cggcgccacc
     1561 cttagcctca ggcacgtaga tggtggccat gccgagggca gcaagctcac gaatgacatc
     1621 gacagggaaa tggcactttt catcccactc ggccgcgaaa ggggcgatgc gctcctgggc
     1681 gaaggcgagt gccgtctccc ggattgcggc ctgctcctcg ttgaggctga agtccatcgg
     1741 ttcatatcct ctcaaacagc gaggggccgt acggcgcgaa gcggccaccc accagctctc
     1801 ggtcggatgg cagcgaaacc tgcccgcaac ccccgccgag ccaaacctga agcagtgaaa
     1861 aagccgtcat gcgggccccg gcatgctcag atcgcagcct tggcaggcgt gcagtccgcg
     1921 gcctcgctca tgcgccggcg ccggcaatcg ccggtcctct gcccaacagc gcttcggctt
     1981 gcgccaccat gccttcgagg atgacgccgg ccggttcgat acaggtgatc aggccaatgg
     2041 cctcgccaac gatggtggcg gcgatccgcg gatc
//
LOCUS       DASTMT010000421         1502 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_894075, whole genome shotgun sequence.
ACCESSION   DASTMT010000421 DASTMT010000000
VERSION     DASTMT010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 1502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1502
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_894075"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(209..1009)
                     /locus_tag="VFR00_09850"
     CDS             complement(209..1009)
                     /locus_tag="VFR00_09850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002714696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU0159607.1"
                     /translation="MDLDLKGKAAIVTGGSRGIGKAIALGLAKEGANIAIVARDRGAL
                     EAARSEIAGATGMQVQAYSCDTGDDGAVKTMVAEAVAALGRVDILVNCAAQPGGQGKP
                     PALGEITNEHFWADMNIKVMGYLRTSREVAPHMIKAGSGRIIHVSGLAARSTGTIIGS
                     MRNVSVAALTKNMADELAPHGIAVVCVHPGLTRTEKTPGVIAAQAKAQGVSAKDIEAR
                     MASRNLARKIITAEEVADVVIFLASPKAVAINGDAIGAGGGVPGVIHY"
     gene            complement(1121..>1502)
                     /locus_tag="VFR00_09855"
     CDS             complement(1121..>1502)
                     /locus_tag="VFR00_09855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018643955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU0159608.1"
                     /translation="PELLPQADIVALTCPLTPQTENLIDAKALALLKPSATLINVARG
                     RVVDEEALLTALQHKRLAAAGLDVTREEPLPAASPLWSMPNVLITPHTAGETQRYEDA
                     VIDILLENLERMWRGESSLRNQVI"
BASE COUNT          234 a          503 c          501 g          264 t
ORIGIN      
        1 ggtcgagggc tcgaagcgag gctgagtttc aagcgcccct gtatcagcgg cgccgtcggc
       61 gcacccgccc ccttcggcaa atccggcacc gcccgtggcc tgccgtgacg ccagggtagc
      121 gccaggatgg cgcagccgtg cccccgccgc cgcccggtct tgcggcgcca caaatgccaa
      181 gggcgaggcg agcgcggctt ggcgcggctc agtagtggat tacgcccggc acgccgccgc
      241 cggcgccgat ggcatcgcca ttgatggcga ccgccttggg cgaggcaaga aagatcacga
      301 cgtcggcgac ctcctcggcg gtaatgatct ttcgcgccag gttacggctg gccatgcggg
      361 cttcgatgtc cttggcggag acgccctggg ctttagcctg cgcggcgatc acgcccggcg
      421 ttttttcggt gcgagtgagg cccgggtgca cacaaaccac ggcaatgccg tgcggcgcga
      481 gctcatcggc catgttcttg gtcaacgccg ccaccgacac attgcgcatc gaaccgataa
      541 tggtgccggt cgagcgagcg gcaaggccgc tgacgtgaat gatgcgaccg gagccggcct
      601 tgatcatgtg cggggcgacc tcgcgcgagg tccgcaaata tcccatcacc ttgatgttca
      661 tatcggccca aaaatgctcg ttggtgatct cgcccagggc aggtggcttg ccttggccgc
      721 cgggctgggc ggcgcagttg accagaatgt cgacgcggcc caaggctgca accgcctcgg
      781 ccaccattgt tttcacggcg ccgtcgtcgc cggtgtcgca actgtaggcc tggacctgca
      841 tgcccgtggc gccggcgatc tcgctacgcg ccgcctcgag ggcaccgcgg tcgcgggcga
      901 cgatggcgat gttggctccc tccttcgcca gccccagtgc gatagccttg ccgatgccgc
      961 ggctgccgcc ggtgacaatg gccgctttgc ccttgagatc gagatccatg tgcgcgccca
     1021 cccctgttgg tttagctatg cctgcaatac tcgggaagga tcggccgcgc tgtcaacagc
     1081 gcaaccgcca gcggcgggcg atcgcaagcg catgccgccc tcaaatgacc tggttgcgca
     1141 ggctcgactc gccgcgccac atgcgctcca ggttctccaa caggatgtcg atgacggcgt
     1201 cctcgtagcg ttgcgtctcg cccgccgtat gcggcgtaat gagcacgttc ggcatgctcc
     1261 aaagcggcga ggcggccggc agtggctcct cgcgcgtcac gtcgagcccg gcggcggcaa
     1321 gtcgcttgtg ctgcagcgcc gtgagcaatg cctcctcgtc gaccacccgg ccacgtgcga
     1381 cattgatcag agttgcagat ggcttcagca gagctagcgc tttggcatcg attaggttct
     1441 ccgtttgcgg cgtgagcgga caggtgagcg cgacgatatc ggcctgcggc agcagctcgg
     1501 gc
//
LOCUS       DASTMT010000422         3672 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_567557, whole genome shotgun sequence.
ACCESSION   DASTMT010000422 DASTMT010000000
VERSION     DASTMT010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3672)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3672
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_567557"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..573
                     /locus_tag="VFR00_09860"
     CDS             <1..573
                     /locus_tag="VFR00_09860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0159609.1"
                     /translation="ENWIEQAQILMKGGATAFSRAFDRGSPAAAALPAQPAVAAAMAV
                     EPAATAIEAGMPEPASGPPARPVLLGALRSVRSQAYQNPEPGPEAAYRVGSHNRVVRS
                     SPPQDLKRIRGIGVLIEKRLNAMQIATYEQIANWTAEDIERVSRMLDFKGRIERENWV
                     EQARILASGGATEFSRRVDRGDVETGRYRP"
     gene            584..2236
                     /locus_tag="VFR00_09865"
     CDS             584..2236
                     /locus_tag="VFR00_09865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009760054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEU0159610.1"
                     /translation="MAQRTQNVLFIMCDQLRWDYLSCAGHPALATPNIDALAARGVRF
                     TRAYVQSPVCGASRMSFYTGRYVHSHGAVWNGVPLKLGEMTLGDYLRPLGVRTALVGK
                     THMRADVEGMQRLGIDPDSIIGVRVQECGFDPYERDDGLHAVGPDGRYDPRQPRYNRY
                     LNDMGYAGANPWHDWANAAAGEGNQLASGWAMRNAGKPARVAERDAETPYMTRRAIDF
                     MLEAEAPWCLHLSYIKPHWPYIAAAPYHAMYRASDVLPAVRSPAERVDPHPIYREFMD
                     LKVSRTFARDDVRATVIPVYMGLIKQIDDQLGILFRFMRERGLFENTMIVFTSDHGDY
                     LGDHWLGEKDLFHEPSVKVPLIVYDPSPAADPARGTRCEALVEAIDLVPTFLEALAGD
                     WRQHAHRLEGRSLLPWLHGRQASAWREFAISEYNYAFLPVARSLGVAPRAANLFMILD
                     RRWKYIHAPGFRPMLYDLETDPDELRDLGAEPAYEGERRRLADALASWGLRCAQRTTR
                     SEGEILAAYGKSERRGILIGVWQEDELPLELWSEYLGKDEGG"
     gene            complement(2315..2539)
                     /gene="rpmE"
                     /locus_tag="VFR00_09870"
     CDS             complement(2315..2539)
                     /gene="rpmE"
                     /locus_tag="VFR00_09870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HEU0159611.1"
                     /translation="MKKQDDFHPDYHRIKVTMTDGTEFETYSTYGKEGDTLTLDIDPK
                     THPAWTGGDRHLVDRGGRLSRFKKKFEGMF"
     gene            2829..>3672
                     /locus_tag="VFR00_09875"
     CDS             2829..>3672
                     /locus_tag="VFR00_09875"
                     /inference="COORDINATES: protein motif:HMM:NF013625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HEU0159612.1"
                     /translation="MGQKQALFRIGGGLLLGVGVLGLVCAAARAESDGPWRKWRGSGA
                     AAGLEDPSDREFIREWEATPPKGYPTLSPNNLFPIKAAIIRYTQIVARGGWTALPDLQ
                     MQEGTTGPAVAQLKQHLLATGDLQEPAGNPESFDYAVMKAVRRYQASNGLAPTGIVDK
                     RTIAALNLPAAARLQQLKTNLVRLEQLSRALPRKYVVVNIPAAQIEAIEGDRVITRHA
                     GVVGKPERATPELKSAIHELNFNAVWHLPPTVVEKDLIPKGQELARAGQSVLIKYKID
                     AYGGD"
BASE COUNT          617 a         1223 c         1199 g          633 t
ORIGIN      
        1 gagaactgga tcgagcaggc gcaaattctg atgaagggcg gcgccaccgc gttctcgcgc
       61 gcctttgacc gcggatcacc cgcggctgcg gccttgccgg ctcagcctgc cgttgccgca
      121 gcgatggcgg tcgagcccgc agcgacggcg atcgaggcgg gcatgcccga gcccgcgagc
      181 gggccgccgg caaggcccgt cctgcttggc gccttgcggt cggtgcgctc gcaggcctac
      241 caaaaccccg agccgggccc cgaggcggcc tatcgcgtgg gctcgcacaa ccgggtggtc
      301 cgctcgagcc cgccacagga tctgaagcgg atccggggga tcggtgtctt gatcgaaaag
      361 cgcttgaacg ccatgcagat tgccacttac gagcagatcg ccaattggac ggcggaagat
      421 atcgagcgcg tcagccgcat gctcgatttc aagggccgca tcgaacggga aaactgggtg
      481 gagcaggcgc gcatcctggc gtccggcggc gccaccgaat tctcgcgccg cgtcgatcgc
      541 ggcgacgtcg agaccggccg ctataggccc taggttggct actttggccc agcgcacaca
      601 gaacgttctt tttatcatgt gcgatcagct gcgctgggac tacctgtcct gcgccgggca
      661 tcccgcgctc gccacaccca acattgacgc gttggccgca cgcggggtgc gcttcacgcg
      721 cgcctacgtg cagtcgccag tctgcggcgc ttcgcgcatg agcttctaca cggggaggta
      781 cgtacactcg cacggcgccg tctggaacgg cgttccactc aagctgggcg agatgacgct
      841 gggcgactac ttgcgccccc tcggcgtgcg cacggcgctg gtcggcaaga cgcatatgcg
      901 cgccgacgtc gaaggcatgc agcgcctcgg catcgacccc gattccatta ttggcgtgag
      961 ggttcaagag tgcggctttg atccctacga acgcgacgat ggcctgcacg ccgtcggccc
     1021 cgacgggcgc tatgatccgc ggcagccgcg ctacaatcgc tatctcaacg acatgggcta
     1081 cgccggcgcc aatccctggc atgactgggc caatgccgcc gccggtgagg gcaatcagct
     1141 ggcctccggc tgggccatgc gtaacgccgg taaaccggcg cgcgtggccg agcgcgatgc
     1201 cgaaaccccc tacatgaccc ggcgcgccat cgactttatg ttggaggccg aggcgccctg
     1261 gtgcctgcac ctcagctata tcaagccgca ctggccctat atcgccgccg ccccttacca
     1321 tgccatgtat agagccagcg acgtcttgcc cgccgttcgc tcgccggccg agcgcgtcga
     1381 cccgcacccg atttatcgcg agttcatgga tctcaaggtc tcgaggacct ttgcgcgcga
     1441 tgacgtgcgc gccacggtca ttcccgtcta catgggcctg atcaagcaga tcgacgacca
     1501 gctcgggatt ttgttccgct tcatgcggga gcgcggccta tttgagaaca ccatgatcgt
     1561 ctttacctcc gaccatggcg attacctggg cgaccattgg ttgggcgaga aggacctctt
     1621 tcatgagccc tccgtgaagg tgccgctgat cgtctatgat ccatcgccgg cggcggaccc
     1681 ggcgcggggc acgcgctgcg aggcgctggt ggaggcgatc gacctcgtgc caacctttct
     1741 cgaggccctc gccggggatt ggcgccagca cgcccaccgg ctggagggcc gctcgctcct
     1801 gccctggctg catggccggc aagcctccgc atggcgcgag ttcgccatca gcgagtacaa
     1861 ttatgctttc ctgccggtgg cgcgatcttt aggcgttgct ccccgcgctg ccaacctgtt
     1921 catgatcctc gatcggcgct ggaaatacat ccatgcgccg ggcttccggc cgatgctcta
     1981 tgatctcgag accgatcccg acgaattgcg cgatcttggc gccgagcccg cctatgaagg
     2041 cgagcggcgc cgactggccg atgcgcttgc gagctgggga ttgcgctgcg cgcagcgcac
     2101 cacgcgctcc gagggtgaga tcctggccgc ctacggcaaa tccgagcgac gcggtatcct
     2161 gatcggggta tggcaggaag acgagctgcc gctcgagctg tggagcgagt acctgggcaa
     2221 ggacgaaggg ggctgagccc cggtcctcgc gcgtatccgg cgccaagatc gggctcccga
     2281 taagacccgt gtgtccagcg gctcgcggcc gcggttagaa catgccctcg aatttctttt
     2341 tgaagcgcga gaggcgtcca ccacgatcga ccaggtggcg atcgccgccg gtccaggccg
     2401 gatgcgtctt ggggtcgatg tcgagggtca gcgtgtcgcc ctccttgccg taggtggagt
     2461 aggtctcaaa ctcggtgccg tccgtcatcg tcactttgat acgatgatag tccgggtgaa
     2521 aatcgtcctg cttcttcatc acacacctct cggcgccgcc gcggcggccg gtcccagcga
     2581 aaaaggtgcc gccttgtact ggaacgggcg ccgcaggaca agccgccgcc ctggctggct
     2641 cgccgtcggc cgtttgtgac cgccccgcac caacttgccc aatcccgccg ccctcggtgg
     2701 ttttgcgctt ctcagctcaa cgggaatgcc gcaggttgcg gggctgggaa cccgctccag
     2761 cagttgccgg tcaaggctcg agcgccgcag agcgtgggta agatcctcgc gcggcatggg
     2821 gtcacgggat ggggcagaag caggcgcttt tcaggatcgg cggcgggctg ctgcttggtg
     2881 ttggcgtgct gggcctggtg tgcgccgcgg cccgggccga gagcgacggc ccctggcgca
     2941 agtggcgcgg ctccggcgcg gcggcgggcc tcgaggatcc gagcgatcgc gagttcatta
     3001 gggaatggga ggcaacacct cccaagggct acccgacgct cagtcccaat aacctctttc
     3061 cgatcaaggc ggccatcatc cgctacaccc agatcgtcgc gcgcggtggt tggaccgccc
     3121 tgcccgattt gcaaatgcaa gagggcacga ccggccccgc cgtcgcccag ctcaagcagc
     3181 atttgctcgc gaccggtgac ctgcaggagc cggccggcaa tccggaaagc ttcgactacg
     3241 ctgtgatgaa ggcggtgcgg cgctatcagg cctccaacgg gctagcgccc accgggattg
     3301 tcgacaagcg caccattgcc gccctcaatt tgcccgccgc ggcccgatta cagcagctca
     3361 aaaccaatct tgtccgcctg gagcagctgt cgcgtgcgct gcccaggaaa tacgtggtgg
     3421 tcaacatccc ggcggcgcag atcgaggcca tcgaaggcga tcgcgtcatc acccggcatg
     3481 cgggcgtggt gggcaagccc gagcgggcca cgcctgagct gaagtcggcg atccacgagc
     3541 tcaacttcaa cgccgtgtgg cacctgccgc cgaccgtggt cgaaaaggac ttgatcccca
     3601 aaggccagga attggccagg gccggccaga gcgtgctcat caagtacaag atcgatgcct
     3661 acggcggcga cg
//
LOCUS       DASTMT010000423         5532 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_4715162, whole genome shotgun sequence.
ACCESSION   DASTMT010000423 DASTMT010000000
VERSION     DASTMT010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 5532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5532)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5532
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4715162"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1701)
                     /locus_tag="VFR00_09880"
     CDS             complement(<1..1701)
                     /locus_tag="VFR00_09880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006932162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEU0159613.1"
                     /translation="MSSRYHEVYAAWKRDPAQFWGDAAREIDWYKPWDRVFDAYAGQY
                     GRWFVGAECNTAYNCLDRHVESGRAHQNALIYDSPVTQTIRAYTYLELREEVATLGGV
                     LLDLGVKRGDRVIIYMPMVPEAVIAMLACARIGAIHSVVFGGFAAPELASRIDDSTPA
                     AILSASCGIEPGRVVHYKPLLDGALALAKHKPASILMLQRPMAEASLLAGRDHDWAGR
                     VAEAKARGRKAGCVSLAATDPLYILYTSGTTGAPKGVIRDNGGHLVALKWTMKNHYGI
                     DPGEVFWAASDVGWVVGHSYICYAPLLHGCTTILYEGKPVGTPDAGAFWRVIAQHGCA
                     AMFTAPTAFRAIKKEDPKGALIKRYDLARFRTLFLAGERADPETIKWAENNLKVPVYD
                     HWWQTETGWPICGNPVGLGALPIKYGSPTVPMPGYDLSALDAAGMPVKAGETGTLAVK
                     LPLPPSCLPTLWGNDARFRNSYLAEFPGYYTTSDAGYIDSDGYVFVMARTDDVINVAG
                     HRLSTGQMEEVVARHPDVAECAVVGVADDLKGQIPAGFIVLNAGVNRSPRDIETEVVT
                     L"
     gene            complement(1786..2184)
                     /locus_tag="VFR00_09885"
     CDS             complement(1786..2184)
                     /locus_tag="VFR00_09885"
                     /inference="COORDINATES: protein motif:HMM:NF017868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase family protein"
                     /protein_id="HEU0159614.1"
                     /translation="MNANLFVYGTLMSTAGHGKGAKLAREASLLGPASIQGRLYRIAW
                     YPGAIDSANAAERVHGELYRLADPVASLDWLDAYEGLSGGLHASEYRRVERLVRPLGS
                     AEVTAWVYLYQGDLGPLQLIADGRWAAAVK"
     gene            complement(2384..2824)
                     /locus_tag="VFR00_09890"
     CDS             complement(2384..2824)
                     /locus_tag="VFR00_09890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012383511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3574 domain-containing protein"
                     /protein_id="HEU0159615.1"
                     /translation="MTEKARLLATAAGCLALGAAGLAVYRVALPQTDLAPCRPGAAQM
                     ARTELLFGTGRKDGAAVGDVEWQAFVDSEVTPRFPDGLTVLTGYGQWKSGGSVRKEGS
                     RVLLIWAPRAPDTDARIEAIRDSYKARFEQESVMRVDGLSCVSF"
     gene            2898..3545
                     /locus_tag="VFR00_09895"
     CDS             2898..3545
                     /locus_tag="VFR00_09895"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007614538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HEU0159616.1"
                     /translation="MAQYKLHCFCQSGNSYKVALYLACAELDWEPVFVDFMHGATREP
                     KWRESVNEMGEAPVLETTAGKLSQSGAILIQLAERTDKFAPQDEAERIEALRWILFDN
                     HKFTSYFASYRFLKSFAPQAPDPSVLAFLQARFESAAGILDKHLGQSKFAVGSRPTIA
                     DFSLAGYVFYPLEEHGYDWAKSHPNLHAWRERLRALPGWKDPYQLMPGERIKPLR"
     gene            3555..4541
                     /locus_tag="VFR00_09900"
     CDS             3555..4541
                     /locus_tag="VFR00_09900"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020092420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HEU0159617.1"
                     /translation="MAISTRLTERLKIKHPIISAPMDGIAGGQLAAAVSRAGGLGMLG
                     GGYSDDAAWFEREFRAAGNHAVGCGFITWALRSNPQLFEAVLARRPKAIFLSFDDPEP
                     FASRARADGIVLFCQLQTRADAERALDCGADVLVAQGTEAGGHGGARATFTLVPEIAD
                     LIAKRAPATLLCAAGGIADGRGLAAALMLGADGVVVGTRFWAAAEALVPAPLQLAALE
                     ADGDATVRQKVLDIVRARAWPERYTGRVLRNDFVREWLGREEALRADQSRQSARYAAA
                     AGAADPGIAATIVGECIGLIDGIEPAGEIVGRMVAEAERLLAKGAGLAGARA"
     gene            4564..>5532
                     /locus_tag="VFR00_09905"
     CDS             4564..>5532
                     /locus_tag="VFR00_09905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011315381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEU0159618.1"
                     /translation="MHFILTEEQAAIQETALAFAKERIAPYAAEWDEKCHFPVDVIRE
                     LAALGMASIYVPEAKGGSGLSRLDGALIMEALAYGCPAISGYVSIHNMVAWMVATYAS
                     DAQIAAWMPKLASMQWLSSYCLTEPSAGSDAAALKASAQPCGDHYLLNGSKQFISGAG
                     ASDFYFVFARTGEEGANGISAFAVMKDSPGLSFGSNEKKMGWNAQPTRQVIFDSCKVP
                     LSNLIGAPGRGFRFAMSGLDGGRINIAACSLGAAWMALDKARAYMLERAAFGRPLADF
                     QALQFKLADMATDLEAARLMTYRAADALDRADPQASMYSAMAKRSAT"
BASE COUNT          929 a         1832 c         1822 g          949 t
ORIGIN      
        1 cagggtgacg acctcggttt cgatgtcccg tggcgaacgg ttcaccccgg cgttgagcac
       61 gatgaacccg gcggggatct gtcccttcaa gtcatcggcg acgccgacaa cggcacactc
      121 ggccacgtcg gggtggcgcg ccaccacctc ttccatctgt cccgtcgaga ggcgatggcc
      181 ggccacattg atgacgtcgt ccgtgcgagc catgacaaag acgtagccgt cgctgtcaat
      241 gtagccagcg tcggaggtcg tgtagtagcc cggaaactcg gcaagatagc tgttgcggaa
      301 acgcgcatcg ttcccccata gcgtcggcag gcaggacggc ggaagcggca gcttgacggc
      361 gagcgtgccg gtctcccccg ctttgaccgg catacccgcg gcgtcgagcg cgctgaggtc
      421 atagcccggc atcggcacgg tgggcgagcc gtacttgatc ggcaaagcac cgagcccgac
      481 gggattgccg cagatcggcc agcccgtttc ggtttgccac cagtggtcgt agacgggcac
      541 cttgagattg ttttccgccc acttgatggt ttccgggtcc gcgcgctcgc cggcaaggaa
      601 cagggtgcga aagcgagcga gatcatagcg cttgatcaat gcgcccttgg ggtcctcttt
      661 cttgatcgcg cgaaaggcgg tgggtgcggt gaacatcgcc gcgcagccat gctgcgcaat
      721 cacccgccag aaggcaccgg catcgggcgt gccaaccggc ttgccctcat agaggatggt
      781 ggtgcagccg tgcagcagcg gcgcatagca gatgtaggaa tggccgacga cccagccgac
      841 gtcggaggcg gcccagaaga cctcgcccgg atcaatgccg tagtggttct tcatggtcca
      901 cttgagggcc accaggtgtc cgccgttgtc gcgtatcacg cccttcggcg cccccgtggt
      961 gccggacgtg tagagaatat agagcggatc ggtggccgcg aggctgacac aacccgcctt
     1021 gcgtccccgc gccttggcct cggcaacgcg gccggcccag tcgtgatcgc gccccgccag
     1081 caggctcgcc tccgccatcg gccgctgcag catcaagatg ctcgcgggct tgtgctttgc
     1141 gagcgccagg gcgccatcga gcagcggttt atagtgcacc acccggcccg gctcgatgcc
     1201 gcaggaggcg gaaaggatcg ccgccggggt tgaatcatcg atgcgggacg ccagctccgg
     1261 ggcggcaaag ccgccgaaca ccaccgagtg aatggcgccg atacgcgcgc aggcgagcat
     1321 ggcgatgacg gcctccggca ccatcggcat atagatgatg acgcgatccc cccgcttgac
     1381 gccgaggtcg agcagcacgc cacccagcgt cgccacctcc tcgcgcagct ccagataggt
     1441 gtaggcccgg atggtttgtg tgaccgggct gtcgtagatc agcgcgttct ggtgcgcgcg
     1501 cccgctctcc acatggcgat cgaggcaatt gtaggccgtg ttgcactccg cccccacgaa
     1561 ccagcgcccg tattggccgg cgtaggcgtc gaacacgcga tcccatggtt tgtaccagtc
     1621 gatttcgcgc gcggcatcgc cccagaactg ggcgggatcg cgcttccagg cggcgtaaac
     1681 ctcgtgatag cggctcgaca tggcatttcc ttttcgcacc cagcaggagt gcggcccgtg
     1741 ctggtcagcg gtcatactgc caagcatcgc cccgaatgca acgggctatt tgacggctgc
     1801 ggcccagcgc ccatcggcga tcagctgcaa ggggccaaga tcaccttggt agagatagac
     1861 ccaggcggtg acctcggcgc tgccgagcgg ccgcacaagc cgctcaacgc gtcgatactc
     1921 gcttgcgtgc agcccgccgc tcaggccctc ataggcatcg agccagtcca agctcgccac
     1981 cggatcagca agccggtaga gctcaccatg gacgcgctcc gcggcattcg cgctatcgat
     2041 ggcgcccgga taccaggcaa tccggtaaag ccggccctgg atcgaggcgg gtccaagcaa
     2101 gctcgcctcg cgcgcgagtt tggcgccctt gccatggccg gccgtggaca tcagcgttcc
     2161 gtagacgaag aggtttgcgt tcatctgagc tcaattctgc gaggcgccag cgcgcggccg
     2221 cgggagtgcc gcgccgccgc atcggcccga tgggaagttg tcgatctgcc tcgatctgcc
     2281 atgacagggg acgcacgccc tgacgctcga gaacccttgc cgcgggcgcc gcttccgcgt
     2341 cggcaagcga ttggtcgagg cccggcccgg cgccccgatg ccttcagaaa ctgacacagg
     2401 ataggccatc gacgcgcatg acgctctcct gctcgaagcg agccttgtag ctgtcacgga
     2461 tcgcctcgat gcgagcgtcg gtgtccggcg cgcgtggcgc ccagatcaag aggacgcggg
     2521 atccttcttt gcgcacgctg ccgccgcttt tccattgacc gtaccccgtc aacaccgtca
     2581 agccatcggg aaagcgcggc gtaacctcgc tgtcgacgaa ggcctgccat tccacgtcgc
     2641 cgaccgcggc cccatccttg cgccccgttc cgaacaaaag ctcagtgcgc gccatctgcg
     2701 ctgcgcccgg ccggcatggc gcgaggtccg tctgcggcaa ggccacccgg tagacggcaa
     2761 gccctgcggc cccgagggcg agacaccccg cggccgtcgc cagcagtctc gccttttcgg
     2821 tcatgagctt tccgctactc ggcggccgat accgcatgct agaaagcgcc cgcggtcgat
     2881 tggagggcgt gagcgccatg gcgcagtaca agctgcattg tttctgccag tccggcaatt
     2941 cctacaaggt tgccctctat ctcgcctgtg ccgagctcga ttgggagccc gtcttcgtcg
     3001 attttatgca cggcgccacg cgggagccca agtggcggga aagcgtaaat gagatgggag
     3061 aggccccggt gctggaaacg acggcgggca agctcagcca gtcgggtgcc attcttattc
     3121 agctcgccga gcgcacggac aagttcgccc cgcaagacga agctgaacgg atagaggcgc
     3181 tgcgttggat cttgttcgat aatcacaagt tcaccagcta ttttgcttcc tatcgctttt
     3241 tgaagtcctt tgcgccgcaa gcgcccgacc cgagcgtgct ggcgttcttg caggcccgct
     3301 tcgagagcgc ggcgggcatc cttgacaagc atttgggaca atccaagttc gcggtcggca
     3361 gcaggccgac gatcgcggac ttctcgctgg cgggctacgt cttctatccg ctggaagagc
     3421 acggctatga ctgggcaaaa agccacccca acctgcatgc ctggcgcgag cgcctgcgcg
     3481 ccttgcccgg ctggaaagat ccctatcaac tcatgcccgg cgagcgcatc aagccgctgc
     3541 gatagatggc aaggatggcg atatcgaccc ggttgaccga acggctaaag atcaagcatc
     3601 ccatcatttc agcgccgatg gatgggatcg ccggcggcca gcttgctgcg gcggtatcgc
     3661 gggccggcgg tctcggcatg ctgggcgggg gctacagcga cgatgcggcc tggttcgagc
     3721 gcgagtttcg cgccgccggc aaccacgcgg ttggctgcgg gttcatcacc tgggctttgc
     3781 gcagcaatcc ccaactgttc gaggccgtgc tcgcgcgcag gcccaaggcg atcttcctgt
     3841 ccttcgacga tccggagccc ttcgccagtc gggcgcgggc cgacggcatc gtgctcttct
     3901 gccagctgca gacccgcgcc gacgccgagc gtgcgctcga ttgcggcgca gatgtgctcg
     3961 tggcccaagg gacggaggct ggggggcacg gcggcgcccg cgccaccttc acgctggtgc
     4021 ccgagatcgc cgacctgatc gccaagcgcg ctccggcgac gctattgtgt gcagccggtg
     4081 ggatcgccga cggccgcggc ttggccgcgg ccctgatgct gggcgccgac ggggttgtgg
     4141 tcggtacgcg cttttgggcc gccgccgagg cgctggtacc ggcaccgctg cagctggcgg
     4201 cgctcgaggc cgacggtgat gcgacggtgc gccagaaggt attggacatc gtacgcgccc
     4261 gcgcctggcc ggagcgctat accgggcggg tcttgcgcaa cgacttcgtg cgcgagtggc
     4321 tggggcgcga ggaggcactg agagccgacc aatcccggca gagtgcgcgc tatgcggccg
     4381 ccgccggcgc ggccgatcct ggcatcgccg ccaccatcgt gggcgaatgc atcggcctga
     4441 tcgatggcat cgaaccggcc ggtgagattg tcgggcgcat ggtggccgag gccgaacggc
     4501 tgctggcaaa gggcgcgggg ctcgcaggcg cgcgagcatg acggcgcgga ggacataggg
     4561 cccatgcact ttatcctcac cgaggaacag gccgccattc aggaaacggc gctcgccttt
     4621 gccaaggaac gcattgcccc ctacgcggcc gagtgggacg aaaagtgcca ctttcccgtt
     4681 gatgtcattc gcgagctcgc tgccctcggg atggcgagca tctacgtgcc ggaggccaag
     4741 ggtggctcgg gcctgtcgcg cctcgatggc gccctcatca tggaggcgct cgcctacggc
     4801 tgtccggcga tctctggcta cgtctcgatc cacaacatgg tggcgtggat ggtggccacc
     4861 tacgcctccg acgcccaaat cgccgcctgg atgcccaagt tggcctcgat gcaatggctg
     4921 tccagctatt gcctgaccga gcccagcgcc ggctccgatg cggccgcgct caaggcctcg
     4981 gcgcaaccct gcggcgatca ctacctgctc aatggcagca agcagttcat ctccggggcg
     5041 ggggcgagcg acttttattt cgtgttcgcg cgcaccggcg aggagggggc caacgggatc
     5101 tcggcttttg ccgtaatgaa ggatagcccc ggcctctctt tcggcagcaa cgagaaaaaa
     5161 atgggctgga atgcgcaacc cacccgccag gtgattttcg acagctgcaa agtccccctc
     5221 tctaacttga tcggcgcgcc tggacgcggc ttccgctttg ccatgagcgg gctcgacggc
     5281 ggccgcatca acatcgctgc ctgttcgctc ggggcggctt ggatggcttt ggacaaggcc
     5341 cgcgcctaca tgctcgagcg cgccgcgttc ggccggccgc tcgccgactt tcaggcgctg
     5401 cagttcaagt tggcggacat ggcgacagat ctcgaggccg cgcggctgat gacctatcgc
     5461 gccgccgatg cgctcgatcg tgccgatccc caggcgtcaa tgtactcggc catggcgaag
     5521 cgttctgcca cg
//
LOCUS       DASTMT010000424         1735 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_5698396, whole genome shotgun sequence.
ACCESSION   DASTMT010000424 DASTMT010000000
VERSION     DASTMT010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 1735)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1735)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1735
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5698396"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..257)
                     /locus_tag="VFR00_09910"
     CDS             complement(<1..257)
                     /locus_tag="VFR00_09910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="3-alpha,7-alpha,
                     12-alpha-trihydroxy-5-beta-cholest-24-enoyl-CoA hydratase"
                     /protein_id="HEU0159619.1"
                     /translation="MLFDPQKILNWKFPEVEHSYSERDTMLYALGLGCGSDPNEAGDL
                     KYVYERGLVALPTMAVVLAYPGNWLESKESTADYSKVLHGE"
     gene            complement(296..1489)
                     /locus_tag="VFR00_09915"
     CDS             complement(296..1489)
                     /locus_tag="VFR00_09915"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016843588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEU0159620.1"
                     /translation="MTIAGPLSGITVIDLTRVLAGPFCTFLLADLGARVIKVEQPGKG
                     DDSRAYGPFINGKSGYFVAQNRGKESIALDLKAAGDVAILHRLLRKADVLVENFRPGA
                     MDRLGFGFAAVHARRPSLVYCSISGFGSTGPQKDRPGYDVIVQGEAGVMDLTGPQDGA
                     PYKVGTAIGDLVSGLTASQGILAALYAAKATGKGQHVSVSMYEVVAALLTFNASIYFA
                     TGQSPRRRGNEHPTIVPYETFEASDGWINLGVANDELWQRFCRGAERPDLAEDSRFAK
                     ASDRVRNRDALVPLIRALIKERSRDDWMARMDKAGVPSGAIRSVGEVCDGDLLSARDM
                     IAAMPHPNAGTVKAVKNAMHLSATPLDGYAAPPTLGQHTREVLTGLLGYAAADVEKLA
                     RAKVI"
BASE COUNT          317 a          559 c          555 g          304 t
ORIGIN      
        1 tgctcgccgt gcagcacctt gctgtaatcg gccgtcgatt ccttgctctc gagccaattg
       61 cccggatagg cgaggacgac ggccatggtt ggcagcgcca ccaagccgcg ctcatagaca
      121 tacttgaggt cgcctgcctc attggggtcg gagccgcagc cgagcccaag cgcatagagc
      181 atggtgtcgc gctcgctgta gctgtgctcg acctccggga acttccaatt caggattttc
      241 tgtggatcga agagcatagg acgcttcccc ctgcttgagc aatcgaaagg gcggctcaga
      301 tcaccttggc gcgcgcaagt ttctccacgt cggcggcggc ataacccaat aggccggtca
      361 atacctcgcg cgtgtgctgt cccagcgtgg gtggcgcggc gtagccatcg agcggcgtgg
      421 cgctcaagtg catggcattc ttgacggcct tgacggtgcc cgcgttgggg tgcggcatgg
      481 ccgcgatcat gtcgcgcgcg ctcaataagt cgccatcgca aacctccccc acgctgcgaa
      541 tcgcgccgct cggcacgccc gccttgtcca tgcgggccat ccagtcatcg cggctgcgct
      601 ctttaatgag ggctctgatc aatggcacca gggcatcgcg attgcgcacc cggtcggagg
      661 ccttggcgaa gcgggagtcc tcggccaaat cgggccgctc tgcgccccgg cagaaccgtt
      721 gccacagctc atcgttggcc acgcccaggt tgatccagcc gtcgctggcc tcgaacgtct
      781 cgtaaggcac gatggtggga tgttcattgc cgcgccggcg cggcgactgg cccgtagcaa
      841 aatagatact ggcgttgaag gtcaaaagcg ccgccaccac ctcgtacatc gagacgctga
      901 catgctggcc tttgccggtt gccttggcgg cgtagagcgc ggccaaaatc ccctgcgagg
      961 ccgtgagccc cgacaccaaa tcgccgattg cggtgccgac cttgtagggc gccccgtcct
     1021 ggggaccggt caagtccatg acgccggcct cgccctgcac aatgacgtca tagccggggc
     1081 gatccttctg cgggcccgtg gagccaaagc cggagatcga gcagtagacg agggatggac
     1141 ggcgggcgtg cacggcggca aagccgaacc caagccgatc catggcgccg ggccggaaat
     1201 tctccaccag cacatcggcc ttgcgcagca gccggtgcag gatggcgacg tcgcccgcgg
     1261 ccttgagatc gagcgcgatc gactccttgc cgcggttctg cgccacgaaa tagccggatt
     1321 tgccgttgat gaaggggccg taggcgcgcg aatcatcgcc cttgcctggc tgttccactt
     1381 ttatgacgcg ggcgccgagg tcggcgagca gaaaggtgca gaacgggccg gcgagcaccc
     1441 gcgtcagatc gatgacggta ataccggaaa gcggtcccgc aattgtcatg cgtgtcagga
     1501 atccacaata aaactcccca gtcggttcat atcggaatcg ccgggccgaa gccagtccgg
     1561 tcgcatcgac aaggccggat ggttccgcta ataaacgcgg aaagagcgcg gccggagagg
     1621 agcaccatgt cgtccaggga gttcgccgac gttccggcca tgctgaaggc gctgacgccg
     1681 agctatccgg tgtactgcct gcggccccat gtcatcgccg acaccgcccg gcgtt
//
LOCUS       DASTMT010000425         2720 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_1223869, whole genome shotgun sequence.
ACCESSION   DASTMT010000425 DASTMT010000000
VERSION     DASTMT010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2720)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2720)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2720
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1223869"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..360
                     /locus_tag="VFR00_09920"
     CDS             <1..360
                     /locus_tag="VFR00_09920"
                     /inference="COORDINATES: protein motif:HMM:NF013625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HEU0159621.1"
                     /translation="LNVGTYVQSKVLIEDGRVIKRLKEDMVANTAVNSAAFAGKLAIF
                     RHAAILGSPAAIRALQRSLQQRELYAGAIDGTYGASLRAAIAAYESAEGLPVTGLATE
                     TLLHRLTGERPAPPRSP"
     gene            complement(380..835)
                     /locus_tag="VFR00_09925"
     CDS             complement(380..835)
                     /locus_tag="VFR00_09925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007196787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein"
                     /protein_id="HEU0159622.1"
                     /translation="MLAALVATPAAAVEPDEMLADAALEARARTLSQELRCLVCQNQS
                     IDDSNAALAKDLRLIVRERLRAGDSDAAVLAFVEARYGEFVLLRPKFNAHTLLLWLTP
                     LLLLLAALFFVLKRSRGGAAAADALAPLSPAEQQRLDELVKPKRTQRGA"
     gene            complement(920..>2720)
                     /locus_tag="VFR00_09930"
     CDS             complement(920..>2720)
                     /locus_tag="VFR00_09930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HEU0159623.1"
                     /translation="AFLTSDFSVEAVWANSHTAKPLIYKFSGVWGNHEGSMLLWVLIL
                     ALFGAAVALFGGNLPDTLRANVLAVQAAIAVCFLVFIVFASSPFARIPPQVEGRGLNP
                     ILQDPALAFHPPFLYGGYVGLSMAFSFAIAALLEGRTDAAWARWVRPWTLAAWMLLTL
                     GIAMGSWWAYYELGWGGWWFWDPVENASFMPWLAATALLHSALVMEKREALKVWTILL
                     AILAFSLSLIGTFLVRSGILTSVHAFAVDPRRGVFILAILTLQIGGALALYAWRAPQL
                     KQGGLFAPISREGSLVLNNVLLTVACATVFIGTLYPLALEAITGAKISVGPPYFNLTF
                     APLMVPLLLAMPLGPLLAWKRGDLVGATERLAAAAALALLAGCITLALKWRGPWLAPF
                     AIALGAWVIAGPLCEWAARVKLFAADRAEVWRRALGLPRSAYGGMLAHAGVGLTVIGI
                     VATSAWRAEHVVVLRPGEGTEIAGYALNFRGVAPRQGPNYQEVVGLFAVTRNGQADGE
                     LAPSKRSFDTPQQTTTEAAIQVGWRGDLYVVLGDEQPGGAWVVRLYFNPLVRLIWLGA
                     VLMALGGLLSLSDRRLRIGVPRRARRGLVQAAE"
BASE COUNT          532 a          915 c          936 g          337 t
ORIGIN      
        1 ctcaacgtcg gtacctatgt gcaatcgaaa gtgttgatcg aggacgggcg ggtcatcaag
       61 cgcctcaagg aggacatggt ggcaaacacg gccgtcaaca gcgccgcctt cgccggcaaa
      121 ctcgcgatct tccggcatgc cgccatcctg ggctcgcccg ccgcgatacg cgcgttgcag
      181 agaagcttgc agcagcgcga gctctatgcc ggcgcgatcg acgggaccta cggggcaagc
      241 ctgcgggcgg cgattgcggc ctacgaaagc gccgagggcc tgccggtgac gggccttgcc
      301 accgagacac tgttgcaccg gctcaccggc gaacgcccgg ccccgccgcg gtccccctga
      361 cggcggcctg ccggcagcct catgcccccc gctgcgtgcg cttgggcttg acgagctcgt
      421 caagacgctg ctgttcggcc gggctcagcg gcgcgagcgc gtccgcggcg gcggcgccac
      481 cccggctgcg tttcagaacg aagaagagcg cggccagcag caacagcaag ggggtgagcc
      541 acagcaatag cgtatgcgcg ttgaatttgg ggcgcagcag cacgaattcg ccgtagcgtg
      601 cctccacgaa ggccagcacg gcggcgtcgc tgtcgcccgc gcgcaggcgc tcgcgcacga
      661 tgagccgcag atctttggcg agcgccgcgt tggaatcgtc gattgactgg ttctggcaaa
      721 ccagacagcg cagctcctgg gaaagcgtac gcgcgcgcgc ctcgagcgcg gcatcggcca
      781 gcatctcgtc gggctcgacg gcggccgccg gcgtggcgac cagcgccgct agcacaacga
      841 ggagccaggc ggcacgccgg agccgcctcg gcgcggcccc cggggtgaca gccaatcgtg
      901 tcgaggcatg ccgcatggct tactcggcgg cctgcacgag gcctcggcgc gcgcggcgcg
      961 gcacgccgat gcgcaggcgg cgatccgaca acgacagaag ccctcccaat gccatcagca
     1021 cggcgcccag ccagatcagg cgcaccagcg gattgaaata gaggcgcacc acccaagcgc
     1081 cgccgggctg ctcgtcgccg agcacgacgt aaaggtcgcc gcgccagccc acctgaatgg
     1141 cggcctcggt ggtggtctgc tgcggggtat cgaagctccg tttcgagggc gcaagttcgc
     1201 catccgcctg accgttgcgc gttacggcga agagaccgac gacctcctgg tagttgggac
     1261 cttggcgcgg tgccacgccg cggaagttca aggcgtaacc ggcaatttcc gtgccttcgc
     1321 ccggccgcaa aacgacaacg tgctcggccc gccaggccga ggtcgcaacg atgccgatga
     1381 ccgtcagccc gacgcccgcg tgggccagca tccccccata ggccgagcgc ggcaggccaa
     1441 gggcacgccg ccagacttcg gcgcgatccg cggcaaaaag cttgacgcgc gccgcccact
     1501 cgcacagcgg accggcaatg acccacgccc cgagcgcaat ggcgaaaggc gccagccacg
     1561 gtccgcgcca tttgagggca agggttatgc acccggccag cagcgccagc gccgcggcgg
     1621 cggccaagcg ctccgttgcg cccaccaggt caccgcgttt ccaggccagc aagggtccca
     1681 agggcatcgc caacagcagg ggaaccatca gcggcgcgaa ggtcaggttg aagtagggtg
     1741 gtccgacgga gatcttggca cccgtgatgg cctccagcgc caagggataa agcgtgccga
     1801 tgaacaccgt ggcacaggcg accgtgagca gaacgttgtt gaggacgagg ctgccttcac
     1861 ggctgatggg cgcgaacagt ccgccttgct tcagttgcgg cgcgcgccag gcataaagcg
     1921 ccagcgcgcc gccgatctgg agcgtcagga tggccaggat gaagacgccg cgcctgggat
     1981 cgacggcgaa ggcatgcacc gaggtcagga tgcccgagcg caccaaaaag gtgccgatta
     2041 aggagaggga gaaggccagg atggccaaca ggatggtcca gaccttgagg gcctcgcgct
     2101 tttccatcac cagcgccgag tgcagcagcg cggtggcggc gagccacggc atgaaggagg
     2161 cattctcgac cggatcccag aaccaccagc cgccccagcc cagctcgtag taagcccacc
     2221 aggaccccat ggcgatgccg agggtcaaca gcatccaggc cgcgagcgtc caaggcctca
     2281 cccagcgcgc ccaggcggca tccgtgcgcc cctcgagcag ggcggcgatg gcgaaggaga
     2341 acgccatgga aagaccgacg tagccgccat agaggaaggg cggatggaag gccaaggccg
     2401 gatcctgcag gatcggattg aggccgcgac cctcgacctg cggcgggatg cgcgcgaagg
     2461 ggctcgaggc gaagacgatg aacaccagga aacagacggc gatggccgcc tgcacggcga
     2521 gcacgttggc gcgcagggtg tcgggcaaat tgccgccgaa cagggcgacg gcggcgccga
     2581 acagcgccag gatcaggacc cacagcagca tcgagccttc atggttcccc cagacgccgg
     2641 aaaatttgta gatcagcggc ttagccgtat gcgagttggc ccacaccgct tccaccgaga
     2701 agtcggaggt gagaaacgca
//
LOCUS       DASTMT010000426         5234 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_2531980, whole genome shotgun sequence.
ACCESSION   DASTMT010000426 DASTMT010000000
VERSION     DASTMT010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 5234)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5234)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5234
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2531980"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1005)
                     /locus_tag="VFR00_09935"
     CDS             complement(<1..1005)
                     /locus_tag="VFR00_09935"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0159624.1"
                     /translation="MNRFVILATGFLILFVGGGTRFAIGLVLKPIAEELDIGRSAIGL
                     AVAAYLVVTSASMFLAGRLADALSMRLVLVGGLLIGAAGMGLMGVLSAPWQIFLFFGV
                     VFAIGNGIASITPVSLLVSRAFPEKAGLANGVVSAGMSAGQLVVIAAFAVLLAQAGWR
                     ALFFWAALAHLALLPLLLIALPKGLASGQDASSRGQPQASLTLAGAVRTRQFWLLIGI
                     YALCGFDDFFVSTHVVAFAQDRGVEALLAGDLLALMGLVGFAGVIAAGAWSDRAGPAT
                     PAIASFVLRIAAFALILVDQSPLAIAVFALLFGFTFLMTAPLLVLFARDAFGLVNLGT
                     I"
     gene            1383..1898
                     /locus_tag="VFR00_09940"
     CDS             1383..1898
                     /locus_tag="VFR00_09940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011087965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEU0159625.1"
                     /translation="MAKTGSRARATPETTPPESIPIRLTVNGVEAELKIPPWTTLLDA
                     LRDRLDLTGTKKGCDHGQCGACTVLVDGRRVNACLTLAVMKDGASITTIEGLAGNGAL
                     HPMQQAFIDHDAFQCGYCTPGQICSAVGLVGEGKAQTADEIRELMSGNLCRCGAYPNI
                     VAAIQQAMDQS"
     gene            1895..2908
                     /locus_tag="VFR00_09945"
     CDS             1895..2908
                     /locus_tag="VFR00_09945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009025775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HEU0159626.1"
                     /translation="MINFQYARAHDVAEAVRQMGADPAAKLIAGGTNLVDLMKYDVER
                     PSRLIDISRLPLTSIEETAQGLRIGALVSNAELAYHPLIEKRYPLLSSATLAGASAQL
                     RNMASTGGNLLQRTRCHYFYDVATPCNKREPGSGCSALCGINRMHAILGTSEACIATH
                     PSDLCVALAVLEASVEVAGPGGERAIPFAEFHRLPADTPERDTNLAHGEIITAVTLPA
                     RGFSSHYTYLKIRDRLSYAFALVSVAVGLELEGNRIKEGRFALGGVAHKPWRDPAAEA
                     LLRGQEANEATFKAAADAVLREAKGYAHNGFKIELARRALVRALGQAARATPQSQSNK
                     SIR"
     gene            2914..5133
                     /locus_tag="VFR00_09950"
     CDS             2914..5133
                     /locus_tag="VFR00_09950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011087967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEU0159627.1"
                     /translation="MAPYIGTATSRIDGRAKVTGVAKYAAEFNVPRLVHGSVVVSTIA
                     KGRIRSIDTSRARGVKGVLDVLTHAHRPQMAQAAEAYKDDVAPDGTPFRPLMDENIHF
                     NGQPIALALAEDYETARFAASLVVVEYEKLQHATDLARELDKAFPLPHDSFALEPAKP
                     RGDAGGALTAAAVRHDGAYYIPIEHHNPMELFATTAIWEGRGKLTVYDKTQGVQNVQR
                     YLCSVFGMKPSQLRVSSAFVGGAFGSGLRPQYQVVLAVLAARALKRSVRVTLTRAQMY
                     GLCYRPGTLERVALGADKDGKLEAITHEAIGMTSQYEAFARKDTIWSATLYRCANTEF
                     THKLARLDLPSPADMRAPGAATGMYAFECAMDELAVALKLDPLELRYRNYSERDQNTN
                     HPFTSKELRECYAQGAAAFGWDKRQAQPRSMREGAELVGWGMASGVWEALEMDMSARI
                     VLTANGHAEVACATSDIGTGTYTIMAQVAADKLGLPLENVSIKLGDSSLPPSPVEGGS
                     WTAASVANAIAMAADATRKELLRLAKKVPDSPLARAKLGEVTLLDGKIVDSRNGHGAV
                     SIADAMRYGSVDRIERKAATGTREHDAYARNVHSAIFAEVKVDEQLGVVRVTRVVNAV
                     AAGRILNAKTARSQIMGGVVWGIGMALHEETLVDHAFGRVMNANIAEYHVPTNADVHD
                     IQVIFVDEPDDIVNPLGIKGLGEIGIVGVAAAVANAVYHATGKRVRDLPITLDKVVS"
BASE COUNT         1034 a         1776 c         1628 g          796 t
ORIGIN      
        1 gatggtgccg aggttcacaa ggccgaaggc gtcgcgcgca aagagcacca gcagcggtgc
       61 ggtcatcaag aaggtgaagc caaagagcaa ggcaaagacg gcaatggcca ggggtgattg
      121 gtccacaagg atcaaggcga aggccgcaat ccgcaggaca aagctggcga tggccggcgt
      181 tgccggcccg gccctgtcac tccaggcgcc ggcggcaatc accccggcga acccgaccag
      241 ccccataagc gccagcagat cgccggccag cagcgcctcc accccccgat cctgggcgaa
      301 ggccaccacg tgcgtggaga cgaagaagtc gtcgaaaccg cacagagcat agatcccgat
      361 cagcagccag aattggcgtg tcctgaccgc gcctgccaag gtcaaacttg cctgcggctg
      421 cccgcggctt gacgcgtctt gcccgctcgc caaacccttg ggcagcgcga tgaggagcag
      481 cggcagcaag gccaaatgcg ccagcgccgc ccagaagaag agcgcgcgcc agccggcctg
      541 cgcgaggagc acggcgaagg cggcgatgac caccagctgc cccgcactca tcccggcact
      601 gaccacgccg ttggcgaggc cggccttctc ggggaaggcg cggctcacca gcagcgacac
      661 cggcgtgatc gacgcaatgc cgttgccgat ggcaaagacc acgccaaaaa ataggaagat
      721 ctgccacggc gccgatagca cgcccatcag gcccatgccc gcggcgccga taaggagacc
      781 gccgacaagg acgagccgca tactgagcgc atcggcgagg cgtcccgcca ggaacatgct
      841 ggcggacgtg accaccagat aggcagcaac cgccaaaccg atcgcgctac gcccgatatc
      901 gagctcctcg gcgatgggtt ttaggacgag accgatcgca aaccgcgtgc cgccgcccac
      961 gaacaagatc aaaaatccgg tggccaaaat gacgaagcgg ttcatggatg gccaatgcgt
     1021 cgcggcgaga gcgattggcc cctccgaata ggcgcacaat tgaactgccg agacggcctt
     1081 ggcacgagca tacctctttc cggcaacgga ccttgagcga agagtgatct gagcgcgacc
     1141 ttagcaaacg ctggatgacc ggacgcccgc catggcgggg agaaacggca ctccacaaga
     1201 gggtgcgagg ccattctgac ccgggggtag cctcccggca aaatccggag tcttccccct
     1261 ctcggcgcgg cgcgcatctc tcctatgctc gcgccatcgg cgcctggtcg gaacccacag
     1321 ccccctacaa acgtacatgg ggaggacagg ggacttcgcc gcggcaatgg agacggcaaa
     1381 aaatggcaaa gaccggttcg cgggcgcgcg ccacccctga aaccacccct cccgagagca
     1441 tccccatccg attgaccgtc aacggcgtcg aggctgagct caagatcccg ccctggacca
     1501 cattgctcga tgccttgcgc gaccgcctcg atttgacggg aacaaaaaag ggctgcgacc
     1561 acggccaatg cggcgcctgc acggttttgg tcgatggccg tcgcgtcaat gcctgcctga
     1621 ctttggcggt catgaaggac ggggccagca tcaccaccat cgagggcctc gccggcaatg
     1681 gcgcgctgca cccgatgcag caggccttca tcgaccacga cgccttccag tgcggctatt
     1741 gcacccccgg ccaaatctgc tccgccgttg ggcttgtggg cgagggcaag gctcaaacgg
     1801 ccgatgagat ccgcgagctg atgagcggca atctctgccg ctgcggcgcc taccccaaca
     1861 tcgttgcggc catccagcaa gccatggacc agtcatgatt aacttccagt acgcacgggc
     1921 gcacgacgtg gccgaggccg tgcgccagat gggtgccgat ccggcggcca aactcattgc
     1981 cggtggcacc aacctcgtcg acctcatgaa gtatgatgtc gagcgcccat cgcggctcat
     2041 cgacatttcc cgcctgccgc tgacctcgat cgaggaaacc gcacaaggcc tgcgtatcgg
     2101 tgccctcgtc agtaacgccg aactcgccta tcacccgctg atcgaaaagc gctacccgct
     2161 gctgtcgagc gccaccctgg ccggggcttc cgcgcaactg cgcaatatgg cctcgaccgg
     2221 cggcaaccta ctgcagcgca cgcgctgcca ctacttttat gacgttgcca ccccctgcaa
     2281 caagcgcgag cctggcagcg gctgctcggc gctctgcggc atcaatcgca tgcacgccat
     2341 cctcggcacc agcgaagcgt gcatcgccac gcatccctcc gacctgtgcg tggcgctggc
     2401 cgtgctcgag gcgagcgttg aggtcgccgg gcctggcggc gagcgcgcca tccccttcgc
     2461 cgagtttcat cgcttgcctg ctgacacgcc ggagcgcgac accaacctgg cccacggtga
     2521 gatcatcacg gccgtcacgc tgcccgcgcg cgggttttcc agccactaca cgtatctcaa
     2581 gatccgcgat cgcctgtcct atgcgttcgc gctcgtctct gtcgccgtag gcctcgagct
     2641 cgagggtaac aggatcaagg aagggcggtt tgcgctgggc ggggtcgcgc acaaaccctg
     2701 gcgggatccc gccgccgaag ccctgctgcg gggacaagag gccaatgagg caaccttcaa
     2761 ggctgccgcc gatgccgtcc tgcgcgaggc caagggctac gcgcacaacg gtttcaagat
     2821 cgagctggcg cgacgagccc tcgtccgggc gctggggcag gcggcacggg caaccccaca
     2881 gtcgcaatcg aacaagagca tcaggtgaag accatggctc cctatatcgg cacggcgacc
     2941 tcgcgcatcg acggccgtgc caaagtgaca ggtgtggcga aatacgccgc cgaattcaat
     3001 gttcccaggc tggtacacgg cagcgtcgtc gtctcgacga ttgccaaggg acgcatccgc
     3061 agcatcgaca cgagccgcgc gcgcggcgtc aagggcgtgc tcgatgtgct cacccatgcg
     3121 catcgcccgc agatggcgca agccgcggag gcctataagg atgacgtggc accggacgga
     3181 acgccgttcc gccccctcat ggacgagaac attcacttca acggccagcc gatcgccttg
     3241 gcgctcgccg aggactacga gaccgcgcgt tttgccgcct ccttggtggt cgtcgaatac
     3301 gaaaaactcc agcacgctac cgaccttgcc cgagagctcg acaaggcctt ccccttgccg
     3361 cacgacagct tcgccctgga gccggcgaaa ccgcgcggcg atgcgggcgg ggccttgacc
     3421 gccgccgccg tgcgccacga cggggcctat tacatcccga tcgagcacca caaccccatg
     3481 gagctcttcg ccaccaccgc gatctgggag ggtcgaggca aactgacggt ctacgacaaa
     3541 acgcaaggcg tgcagaacgt gcagcgctat ctgtgcagcg tgttcggcat gaagcccagc
     3601 cagctgcgcg tcagctccgc ctttgtcggg ggcgctttcg gctcgggcct gcgcccgcaa
     3661 tatcaagtgg tcctcgcggt gcttgcggcg cgcgccctga aacgctcggt gcgtgtgacc
     3721 ctgacgcgcg cgcaaatgta tgggctttgc tatcgcccgg ggaccctcga gcgcgttgcg
     3781 ctcggtgccg acaaggacgg caagctcgaa gccatcacgc acgaagcgat cggtatgacc
     3841 tcacaatacg aggcgttcgc gcgcaaggat acgatctggt cggctaccct ctaccggtgt
     3901 gccaacaccg aattcactca caaactggcg cgtctcgacc ttccctcgcc ggcggatatg
     3961 cgcgcgccgg gcgccgccac gggcatgtat gcctttgaat gtgcgatgga cgagctcgcc
     4021 gtggccctca agctcgatcc cctggagttg cgttaccgca actattccga acgggaccag
     4081 aatacgaacc atccctttac cagcaaagaa ttgcgtgagt gctacgccca gggggcggcg
     4141 gccttcggct gggataaacg ccaagcgcaa ccccgcagca tgcgcgaggg tgcggagctc
     4201 gtcggctggg ggatggcgag cggagtgtgg gaagccctgg aaatggatat gagcgcccgg
     4261 atcgtcctga ccgccaacgg acatgcagag gttgcctgcg ccacctcgga catcggcacc
     4321 ggtacctaca cgatcatggc ccaggtcgcc gccgacaagc tcggtctccc cttggagaat
     4381 gtcagcatca aactcggcga ttcatcgcta ccgccctcgc ccgtggaggg cgggtcctgg
     4441 accgcggcct cggtcgccaa tgccattgcc atggcggccg atgcaacccg caaagagctg
     4501 ctgcgccttg ccaagaaagt gcccgactcg ccgctggccc gtgccaagct gggcgaggtg
     4561 acgctgctcg acggcaagat cgtcgacagc cgcaatgggc acggcgccgt ctcgattgcc
     4621 gacgccatgc gctacggcag cgtcgatcgc atcgaacgga aagcggcgac cgggacccgg
     4681 gagcacgatg cctatgcacg caatgtgcac tcggccattt tcgccgaggt gaaggtcgac
     4741 gagcagctgg gcgttgtgcg tgtcacgcgc gtcgtgaacg ccgtcgccgc cggccggatc
     4801 ctcaacgcca agacggcgcg gagccagatc atgggcggcg tcgtctgggg catcggcatg
     4861 gccttgcacg aggagaccct cgtcgaccac gccttcggcc gcgttatgaa cgccaatatc
     4921 gccgagtacc acgtgccgac caacgccgat gtccacgaca tccaggtgat tttcgtcgat
     4981 gaacccgacg acatcgtcaa cccgctcggg atcaagggac tgggcgagat cggcatcgtc
     5041 ggcgtcgccg ccgcggtcgc caatgcggtc taccacgcga ccggcaagcg ggtgcgcgat
     5101 ctgccgatca cgctggataa ggtggtttcc taacctcctt tgcttgcatt cgatccggcg
     5161 gaagcccgag tcgacagggt ttccgccgtc cattagcctt ggcttggcca atcaccctat
     5221 tggacggagc cagg
//
LOCUS       DASTMT010000427         6594 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_5046323, whole genome shotgun sequence.
ACCESSION   DASTMT010000427 DASTMT010000000
VERSION     DASTMT010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 6594)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6594)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6594
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5046323"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..547)
                     /locus_tag="VFR00_09955"
     CDS             complement(<1..547)
                     /locus_tag="VFR00_09955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020186328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     permease subunit"
                     /protein_id="HEU0159628.1"
                     /translation="MTAAAAQRAMPALPRRLSGEDLLMRAGIVVLAMLLLLFIALPLA
                     TLLLKSFQDSTGRLIGLANYQRYFMTPALVASLWNSLAVAVLSTAIVVPLAFLYAYGL
                     TRTCVPAKRLFVALALLPLFAPSLLPAISLIYIFGNQGFLKSWLNGASIYGPFGIVLA
                     QVFYCFPHALMILLTAFSLADG"
     gene            complement(544..1683)
                     /locus_tag="VFR00_09960"
     CDS             complement(544..1683)
                     /locus_tag="VFR00_09960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015456682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033225 - ATPase-coupled
                     2-aminoethylphosphonate transporter activity [Evidence
                     IEA];
                     GO_process: GO:0033223 - 2-aminoethylphosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     ATP-binding protein"
                     /protein_id="HEU0159629.1"
                     /translation="MPSVQRADDWEPAKTTAGIAGPPHLSVLGIAKRFGSFSALEDVS
                     LAVAAGKLVCFLGPSGCGKTTLLRVIAGLESQDAGSIMIGGRDVSQLPPAQRDFGIVF
                     QSYALFPNLTVAQNIGYGLVNRRKRRAEIKARVAEMLSLVGLTEHGAKYPIQLSGGQQ
                     QRVALARALATSPSLLLLDEPLSALDARVRVRLREEIKALQRRLGVTTIMVTHDQEEA
                     LALADEIVVMNRGRVEQIGTPTEIYNEPATAFVADFVGETNMLDATILGADRIRVGAL
                     TLTCAHSEMHSPGEQVRLGLRPEDVRIRGLAPGAPNSLVVRVVLLDFLGAFWRATLHV
                     ESDPGLVLRADFSINAMRDLDIHVGQSLTISLPPEALRVFAGAGA"
     gene            complement(1714..2739)
                     /locus_tag="VFR00_09965"
     CDS             complement(1714..2739)
                     /locus_tag="VFR00_09965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018430958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033225 - ATPase-coupled
                     2-aminoethylphosphonate transporter activity [Evidence
                     IEA];
                     GO_function: GO:0033226 - 2-aminoethylphosphonate binding
                     [Evidence IEA];
                     GO_process: GO:0033223 - 2-aminoethylphosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HEU0159630.1"
                     /translation="MFAKLLKVAGVSALLAGLLAAPALAQKTKLTVYTALENDQLAPF
                     KTEIEKAVPGVDIEWVRDSTGVITARFLAEKDNPKADMVMGLAASSLLAFKKAGLLEA
                     YVPKGADALRPAFRSPDGTWTAHDAYVGIICFNTAEADKLGLTAPKSWKDLTNPKYKD
                     KLVMPHPASSGTGYLTVAAWLQLMGEAEAWAFMDALHQNIAVYTHSGSAPCVQAAKGE
                     RVAGIALDMRGAREKTAGAPLEVIAPVEGVGWDMEAYAIVKGSKKLDLAKAVADWAAS
                     KPANELYSKYYAIVAHPEVKNVPKNYPAEAEARMAKIDFQKMADDRERVLAEWSKRYD
                     AKAAPKN"
     gene            complement(2777..4240)
                     /gene="phnY"
                     /locus_tag="VFR00_09970"
     CDS             complement(2777..4240)
                     /gene="phnY"
                     /locus_tag="VFR00_09970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013533038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde dehydrogenase"
                     /protein_id="HEU0159631.1"
                     /translation="MQDRVSKPKLPCKFHEDMRIAGRRVEAQERIEVRNPFTGEVVGT
                     VPAASPAQVAEAFRLGEAYKPRLSRWERQKILLRAAELLAQRREPIATLISMESGLCL
                     KDALYEVGRACDVYGLAGQLAIRDDSEVYACDITQHGKARRIYTSRTPVRGVISAITP
                     FNHPLNMISHKIAPAIATNNRVVAKPTELTPLTALVLADLLYEAGLPPEMLSIVTGNP
                     VSMGDAMITDPRADIVTFTGSVKVGKYIAEKAGYKRLVLELGGNDPLIVMEDADLEKA
                     AELAVQGATKNSGQRCTAVKRILVVEAVADALVERVLAKARQLKCGDPLDPETDIGTV
                     IHERAARQFEARVNDAVAQGAKLLLGNKREGALYPATVVDHVPYRCELVHQETFGPVI
                     PIVRCPNDIAAVIAISNDTAYGLSSGVCTNRLDYITRFINELEVGTVNVWEVPGYRIE
                     MSPFGGIKDSGLGYKEGVWEAMKSYTNVKTYSLPWPA"
     gene            complement(4260..5516)
                     /gene="phnA"
                     /locus_tag="VFR00_09975"
     CDS             complement(4260..5516)
                     /gene="phnA"
                     /locus_tag="VFR00_09975"
                     /EC_number="3.11.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018272035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047400 - phosphonoacetate hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019636 - phosphonoacetate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetate hydrolase"
                     /protein_id="HEU0159632.1"
                     /translation="MTQRRTAEPVTCNGRAYAMPAAPTVVICLDGSEPGYIEETIAAG
                     RAPTFARFLERGANLKAESVIPSFTNPNNLSIITGRPPAVHGIAGNFFYDPATEKEVM
                     MNDPRFLRAPTILKAFHDAGAKVAMVTAKDKLRLLLSDGLTYAGGRAIAFSAERADVV
                     TRERNGIDDALTFVGRPLPNVYSAALSEFVFAAGVRLLAEVRPDLMYLSTTDYVQHKA
                     GPGTPLANDFYAMIDGYLADLDRQGAVIVATADHGMNDKHRQDGTPDVIYLKTLFDAW
                     LGRDQARVILPITDPYVVHHGALGSFATVYLPQGADASDLIGKLEAIPGVDLAVPRLE
                     ACRRFELPPDRIGDIVVISARHKALGTAPELHDLSGLDEPLRSHGGLTEQRVPVLSNR
                     RLAVESGRVLRNYDAFDLALNCVTLN"
     gene            complement(5530..>6594)
                     /locus_tag="VFR00_09980"
     CDS             complement(5530..>6594)
                     /locus_tag="VFR00_09980"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020186333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047304 - 2-aminoethylphosphonate-pyruvate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HEU0159633.1"
                     /translation="VKRAMLHDWGSRDATFLALNTQVLERLVQIINGAGTHVTVPVQG
                     SGTFAVEAMLTSFVPRTGKVLLLINGAYGQRARKICEIAGRDHLVHETAEDTPPDLAA
                     IDRQLDADRNISHLFAVQCETTSGILNPISEIATLAERHGRRLIIDAMSAFGAIPLDA
                     KLTRFDAVAASSNKCIEGVPGLGFVLCREPALAETKGNATTLVLDLHDQWQALVKTGQ
                     YRFTPPIHVIVAFHQALQEFWAEGGQAGRGGRYAENCRVLIAGMRAMGFRPLLRPELQ
                     APIIVTFHMPKHAKFVFPRFYDMLKERGYVIYPGKLTVADSFRIGCIGRLNAEHMRGA
                     LGAIAEVLAELGVAELGPTP"
BASE COUNT         1180 a         2064 c         2200 g         1150 t
ORIGIN      
        1 gcccgtcggc aagggaaaag gcggtcaaga ggatcatcag ggcatgcgga aagcagtaga
       61 agacctgcgc cagcacgatg ccgaaggggc catagatgct ggcaccgttg agccaactct
      121 tgaggaagcc ctggttgccg aaaatataga tgagcgagat ggcgggcagc agcgacggcg
      181 cgaacagggg tagcagcgcg agcgccacga acagccgctt ggcgggcacg caggtccggg
      241 tcagcccgta ggcgtaaaga aaagccaacg gcacgacgat tgccgtcgac aacaccgcca
      301 ccgccaggct gttccagagc gaggcaacca gcgccggcgt catgaagtag cgctggtagt
      361 tggcaagacc gatgaggcgt cccgtactgt cctgaaagct cttcaagagc agggttgcga
      421 gcggcaaggc gatgaacagc agcaggagca tggcgaggac gacgatgccg gcgcgcatca
      481 agaggtcctc gccggacagg cggcgcggca gcgctggcat cgcccgctgg gccgccgccg
      541 cggtcatgct cccgccccgg cgaagacccg caaggcctcg ggaggcaagg agatcgtcaa
      601 gctctgcccg acgtgaatat cgaggtcgcg catggcgttg atggaaaaat cggcacgcaa
      661 taccaagcct ggatcgctct cgacgtggag cgtcgcgcgc cagaacgcgc cgagaaagtc
      721 gagcaaaacc acgcgcacca ccaggctgtt gggcgcgccg ggtgcgagcc cgcggatgcg
      781 cacgtcctct ggccgcaacc ccagccgcac ctgctcgccc gggctgtgca tctcgctatg
      841 cgcgcacgtc aacgtcagcg cgccaacccg aatacggtcg gcgccgagga tagtggcatc
      901 caacatattc gtctcgccga cgaagtcggc gacaaaggcg gtggcaggct cattataaat
      961 ctcggtcggg gtaccaattt gctcgacgcg gccccggttc atgaccacga tctcgtccgc
     1021 cagcgccagc gcctcttcct gatcgtgcgt caccatgatc gtggtgacgc ccaggcggcg
     1081 ctgcagcgcc ttgatctcct cgcgcaggcg cacgcgcacg cgcgcatcga gcgcggagag
     1141 cggctcgtcg agcagcaaga gggagggcga ggtagcgagc gcgcgggcga gcgcaacacg
     1201 ctgctgctgg ccacccgaaa gttgtatggg gtatttggcg ccgtgctcgg tcaacccgac
     1261 gaggcttagc atttcggcca cgcgcgcctt gatctcggcg cgccgcttgc gccgattgac
     1321 gagcccatag ccgatattct gggcgaccgt caggttggga aagagagcgt aggactggaa
     1381 gacgatgccg aagtcgcgct gcgccggcgg cagctggctg acgtcccggc cgccgatcat
     1441 gatgctgccg gcatcctggc tctcgaggcc ggcgatcacg cgcaggagcg tggtcttgcc
     1501 gcagccagac ggcccgagaa agcagacaag ctttcccgcc gccaccgcga gcgagacgtc
     1561 ctcgagggcg ctgaagctgc caaagcgctt ggctatgccc agcacgctca gatgcggcgg
     1621 gccggctatg ccggccgttg tcttggccgg ctcccagtcg tcggcgcgtt ggacgcttgg
     1681 catgggacgg ctcacaccat gagcgcgacg ctctcagttc ttcggggccg ccttggcgtc
     1741 atagcgcttc gaccactccg ccaaaacgcg ctcgcgatca tcggccatct tttgaaagtc
     1801 gatcttggcc atgcgcgctt cggcctcggc gggataattc ttgggtacgt tcttcacttc
     1861 ggggtgtgcc acgatcgcat aatacttgga ataaagctca ttggccggct tgctggcagc
     1921 ccagtccgcc acggctttgg cgaggtcgag ttttttgctg cccttgacga tggcataggc
     1981 ttccatgtcc cagccgacgc cctccacggg ggcaatgacc tccaaggggg cacctgccgt
     2041 cttctcccgc gccccgcgca tatcgagcgc tatcccggcc acgcgttcgc ccttggccgc
     2101 ctgcacgcac ggcgcgctgc ccgaatgggt atagacggcg atgttctggt gcagggcgtc
     2161 catgaaggcc caggcctcgg cctcacccat cagctgcagc caggcggcca cggtgaggta
     2221 acccgttccc gaggaagccg ggtgcggcat caccagttta tccttgtatt tggggttcgt
     2281 gaggtccttc cacgacttgg gtgcggtcaa gccgagcttg tcggcctccg ccgtgttgaa
     2341 gcagatgatg ccgacgtagg cgtcgtgcgc ggtccaggtg ccatccggcg agcggaaggc
     2401 ggggcgcagg gcgtccgccc ccttgggcac ataggcctcg agcaggcccg cctttttgaa
     2461 cgccagcaga ctagaggcgg caaggcccat caccatgtcg gccttgggat tgtccttttc
     2521 ggcaaggaaa cgggcggtaa tgacgccggt ggagtcgcgc acccattcga tgtcgacgcc
     2581 gggcaccgcc ttttcgattt ccgtcttgaa gggcgccagc tgatcatttt ccagggcggt
     2641 gtaaaccgtc agcttggtct tctgcgccag ggcaggcgcg gccagcaagc cggccaagag
     2701 ggcgcttacg cccgccactt ttaagagttt cgcaaacatc gaaccatact cccccagggg
     2761 ttgcgagcct tggccgtcac gccggccagg gcagcgaata ggtcttgacg ttggtgtagc
     2821 ttttcatggc ctcccagacg ccttccttgt agccgaggcc cgaatccttg atgcctccga
     2881 acgggctcat ctcgatgcga tagcccggca cttcccagac attgacggtg cccacctcca
     2941 gttcgttgat gaagcgcgtg atgtagtcga gccggttcgt gcatacgccc gaggacagcc
     3001 cgtacgccgt gtcgttggag atcgcaatca ccgcggcgat gtcgttgggg cagcggacga
     3061 tcgggatgac cggaccaaag gtctcctggt gcacgagctc gcagcgatag ggaacgtgat
     3121 cgacaacggt cgccgggtat aacgccccct cgcgcttgtt gcccagcaag agcttggcac
     3181 cctgggcgac cgcgtcgttg acgcgcgctt cgaattggcg cgccgcacgt tcgtgaatga
     3241 ccgtgccgat atcggtctcg ggatcgaggg gatcgccgca tttcagctgg cgcgccttgg
     3301 ccaatacccg ctcgactaac gcatcggcaa ccgcctccac caccaggatg cgtttgacgg
     3361 ccgtgcagcg ctgaccggag tttttggtgg cgccctgcac cgccagctcg gccgcctttt
     3421 cgaggtcggc atcttccatg acgatcaagg gatcgttgcc cccgagttcc agaacgagcc
     3481 gtttatagcc agccttttcg gcgatgtact tgccgacctt gacactgccg gtaaaggtga
     3541 cgatgtcggc gcgtggatcg gtgatcatgg cgtcacccat gctgacggga ttgccggtca
     3601 caatcgacag catctcgggc ggcagccccg cctcatagag aaggtcggcc agcaccagcg
     3661 cggtcagcgg tgtgagctcg gtcggcttgg ccaccacacg attgttggtg gcaatcgccg
     3721 gggcgatttt gtggctgatc atattgagcg ggtgattgaa cggggtgatc gccgagatca
     3781 cgccgcgcac gggcgtacgc gaggtataga tgcgccgagc cttgccgtgc tgggtgatat
     3841 cgcaggcata gacctcgctg tcgtcgcgga tcgccagctg cccggcgagc ccgtagacgt
     3901 cacaggcgcg accgacctcg taaagcgcat ccttgaggca taggcccgac tccatgctga
     3961 tcagggtggc gatgggctcg cgccgctgcg ccaaaagttc cgccgcgcgc agcaggattt
     4021 tctggcgttc ccagcggctg agcctgggct tataggcctc gcccagccgg aaggcttcgg
     4081 cgacctgcgc cgggcttgcc gcggggacgg tgccgacgac ctcgcccgta aagggattgc
     4141 gcacctcgat gcgctcctgc gcctcgacgc gcctgcccgc gatgcgcatg tcttcgtgaa
     4201 acttgcaggg cagcttcggc ttggaaactc gatcctgcat gaggagcctg ctccctggtc
     4261 tagttcaaag tcacgcagtt caaggcaagg tcgaaggcat cgtaattgcg caagacccgg
     4321 ccagattcaa cggcaagccg ccggttcgac agcacgggca cgcgctgctc cgtcagccca
     4381 ccgtgcgagc gcagcggttc gtcgagacct gagaggtcgt gcagttccgg cgccgtaccc
     4441 agcgccttgt gtcgggcaga gatgacgacg atgtcgccga tccgatccgg cggcaattcg
     4501 aaacggcgac acgcctccag gcgcggcacg gcgagatcga cgccggggat ggcctcgagt
     4561 ttgccaatca ggtcggaggc gtcggcgccc tgcggcagat aaacggtggc aaaggagccg
     4621 agcgcgccgt gatgcaccac gtagggatcg gtgatgggca gaatgacacg tgcctggtcc
     4681 cgacccagcc aggcgtcgaa cagggtcttg aggtagatga cgtcgggcgt tccgtcctgc
     4741 ctgtgcttgt cgttcatgcc gtgatcggcc gtggccacga tcacggcgcc ttgccggtcg
     4801 aggtccgcca aatagccgtc gatcatggcg taaaaatcat tcgccagcgg cgtgccggga
     4861 ccggccttgt gctgcacata gtcggtggtg gacaggtaca tgaggtccgg gcgtacttcc
     4921 gccaggagcc ggacgccggc ggcgaacacg aattccgaca gcgcggccga gtagacgttg
     4981 ggcagcggcc gccccacgaa ggtgagcgca tcatcgatgc cgttgcgctc gcgcgttaca
     5041 acgtcggcgc gctcggcgga aaaggcaatg gctcgtccgc cggcataggt gagcccgtcc
     5101 gacaacagca ggcgcagctt gtctttggcc gtgaccatcg ccaccttcgc gccggcgtca
     5161 tggaaggcct tgaggatggt cggggcgcgc aggaagcgcg gatcgttcat catcacttcc
     5221 ttttcggtcg ccggatcata gaagaaattg ccggcaatgc cgtgcacggc gggcgggcgg
     5281 cccgtgatga tggaaagatt gttggggttg gtgaaactcg ggatcacgct ttcagctttg
     5341 aggttggccc cgcgctccag aaagcgcgcg aaggtcggcg cgcgccctgc cgcaatggtc
     5401 tcctcgatgt agccgggttc ggagccgtcg aggcaaatga caacggtggg cgccgcgggc
     5461 atcgcgtaag cgcggccgtt gcaggtcacg ggctccgcag tccggcgttg ggtcatcgcg
     5521 tggatctcgt catggggtgg ggcccagctc ggctacgccg agctccgcca ggacctcggc
     5581 gatggcgcca agcgcgccgc gcatatgctc ggcgttgagc ctgccgatgc agccaatgcg
     5641 aaaactgtcg gccacggtca gcttccccgg atagatcacg tagccgcgtt ccttgagcat
     5701 gtcatagaag cgggggaaaa cgaacttggc atgcttaggc atatggaatg tgacaatgat
     5761 gggggcctgt agctccggcc gcagcaatgg gcgaaatccc atggcccgca tgcccgcgat
     5821 caggacccga caattctccg cgtagcgccc accccggccc gcctggccgc cctcggccca
     5881 gaactcctgc aacgcctgat ggaaggcaac gatgacgtgg atcggcgggg taaaccggta
     5941 ttggccggtc ttgaccaggg cttgccactg gtcgtgcaag tcgagcacca gggtcgtcgc
     6001 attgcccttg gtctcggcga gcgccggctc gcggcagagc acgaaaccta ggccgggcac
     6061 gccctcgatg cacttgttgg aggaggcggc cacggcatca aaacgcgtca gcttcgcatc
     6121 gagcggtatg gcgccgaagg cgctcatggc atcgatgatg aggcgccggc cgtgccgctc
     6181 tgccagggtc gcgatctcgg agatcgggtt gagaatgccg ctcgtggttt cgcactgcac
     6241 ggcaaagaga tgactgatgt tgcgatcggc gtcgagctgc cggtcgatgg cggccagatc
     6301 cggcggggta tcctccgccg tctcgtgcac gaggtggtcg cgcccggcga tctcgcagat
     6361 tttgcgggcg cgctggccgt aggccccatt aataaggagc agaaccttgc ccgtgcgcgg
     6421 cacaaagctg gtcagcatgg cctcgaccgc gaacgtgccc gagccctgca cgggcacggt
     6481 gacgtgcgta ccggcgccgt tgatgatctg gactaggcgc tccaggactt gggtgttgag
     6541 ggcgaggaag gttgcatcgc gcgaacccca gtcgtgcagc atcgcccgct tcac
//
LOCUS       DASTMT010000428         1753 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_571949, whole genome shotgun sequence.
ACCESSION   DASTMT010000428 DASTMT010000000
VERSION     DASTMT010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 1753)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1753)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1753
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_571949"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..324)
                     /locus_tag="VFR00_09985"
     CDS             complement(<1..324)
                     /locus_tag="VFR00_09985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003598242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEU0159634.1"
                     /translation="MSGLALGSMYGLIALGFHITYVVSNTVNFSQGSSVMLGAVLGYT
                     FAVRLGWPLPLALASALVACAGFGLLVERTLVRPFAERGSNAWLMATVAGGIVLDNVV
                     LFTFGK"
     gene            complement(437..1621)
                     /locus_tag="VFR00_09990"
     CDS             complement(437..1621)
                     /locus_tag="VFR00_09990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015857664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0159635.1"
                     /translation="MLADVAPARIARRCLLAGALAVAAALPAGAQEVVKLGLVAAMSG
                     QSAKSGEAIVRGLALAVDEINAAGGLLGKKVELVVRDDESNPAKGAIAARELVQREKV
                     AALFGGLDTPVSLAIVPFANQNKVPFMGVWAAGTPITRNGAAENYVFRVSAVDELVDK
                     ALVDYSIAKHQAKKPGMILINNPWGESNEKGLKAALAAKNVAYAGIEKFETNDVDVVP
                     QLSRLKEAGADVLSLVANVAPSSQVVKSLDRMGWNVPIVSHWGPAGGRFSELAGASAA
                     KVHFIQTYSFSGKLSPKGEAVLAALKKKYPEVKTLADVTPAVGIANAYDAMHLTALAI
                     AKAQSLEGPKVREAFYAIDSYAGLIKTYQKPFSPENQDALSEKDYLFTHFVEGEILPL
                     TQ"
BASE COUNT          320 a          558 c          570 g          305 t
ORIGIN      
        1 cttgccgaag gtgaacagaa ccacattgtc gaggacaatg ccgccggcca ctgtcgccat
       61 cagccaggca ttcgatccgc gttccgcgaa cgggcgcacc aaggtgcgct cgaccaagag
      121 cccgaagccg gcgcaggcta cgagggcgct cgccagcgcc agcggcaacg gccagccgag
      181 ccgcaccgcg aaggtgtagc ccagcaccgc gcccagcatg accgagctgc cctgcgagaa
      241 gttgaccgtg ttggaaacca cataggtgat gtgaaatccg agcgcgatca ggccatacat
      301 ggagccgagc gccagtccgc tcatgatggc ggaggttacg aggatggttg cccctccggt
      361 cgatggcagg ggatgctcgg cgtcacgggc gcgacgccga tgggatcctc aactgcgcac
      421 atgcggccga caatgcttac tgcgtcagcg gcaggatctc accctccacg aagtgcgtaa
      481 agaggtagtc cttttccgaa agcgcatctt ggttttcggg gctgaacggc ttttggtagg
      541 ttttaatcag gccggcgtag ctgtcgatgg catagaaggc ctcgcgcacc ttggggccct
      601 cgagggactg cgccttggcg atcgccagcg cggtaaggtg catggcgtca taggcgttgg
      661 cgatgccgac cgcgggcgtc acgtcggcca gcgtcttgac ttccggatac ttctttttca
      721 aggccgccag caccgcctcg cccttgggcg aaagcttgcc cgagaagcta taggtctgga
      781 taaagtgcac cttggcggcg ctggcgccgg ccagctcgct gaagcggccg ccggccgggc
      841 cccagtgcga cacgatcggc acgttccagc ccatgcggtc gagcgacttc acgacctggg
      901 aggaaggtgc gacattggcg acgagggaga gcacgtcggc gccggcctcc ttgaggcggc
      961 tcagctgtgg caccacgtcg acgtcgttgg tttcgaactt ctcgatgccg gcgtaggcaa
     1021 cgttcttggc ggcgagcgcc gccttgagac ccttttcgtt ggattcgccc caagggttgt
     1081 tgatcaggat catgccgggc tttttcgcct ggtgcttggc gatcgagtag tcgaccagcg
     1141 ccttgtcgac caattcatcc accgccgaaa cgcgaaagac gtagttctcc gccgcgccgt
     1201 tgcgcgtgat cggcgtgccg gccgcccaaa cgcccatgaa ggggactttg ttctggttgg
     1261 cgaacggcac gatcgccagc gacaccgggg tgtcgaggcc gccgaacagg gccgcaacct
     1321 tctcgcgctg gaccagctcg cgtgccgcaa tggctccctt ggcgggattg ctttcgtcat
     1381 cgcgcacgac gagttcgact tttttgccca acagaccgcc ggcggcgttg atctcgtcga
     1441 cggcgagggc gagcccgcgc acgatcgcct caccggattt tgccgattgc cccgacatgg
     1501 ccgcgaccaa accgagcttc accacctctt gcgcgccggc gggaagcgcg gcggcaacgg
     1561 cgagcgcgcc cgcgagcagg caacgccggg cgattcgagc aggcgcaacg tcggcaagca
     1621 tggcaaagcc ccttttcgca agacgggaag atggatgccc ctggcagtga ccccctggga
     1681 gagcaaagca tatgccacac gccccggtcc atgcctacga tttattggcg caaatctgcc
     1741 caagttaggg gca
//
LOCUS       DASTMT010000429         2244 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_6031210, whole genome shotgun sequence.
ACCESSION   DASTMT010000429 DASTMT010000000
VERSION     DASTMT010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2244)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2244)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2244
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6031210"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..930
                     /locus_tag="VFR00_09995"
     CDS             <1..930
                     /locus_tag="VFR00_09995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013949759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HEU0159636.1"
                     /translation="RDRRSAADALAAADTAHRSAAQELRAAQTAVADERERRARTEAH
                     LENARQRRAEEARKIREQLGCSPEDCLALADVAPATPLPTLETAVGGLNRLKAERERL
                     GGVNLQADDDLAGLSQQFESLDKEKRDVEEAIAKLRAGIAQINNEGRTRLQAAFDAVN
                     AHFKQLFMTLFGGGEARLEMVDAEDPLDGGLEIIARPPGKKPATLSLLSGGEQSLTAM
                     ALIFAVFLTNPSPICVLDEVDAPLDDANVDRFCTLLEKMAADTATRFLVITHHPMTMS
                     RMNRLFGVTMVEKGVSQLVSVDLETAQKFVVAA"
     gene            959..1612
                     /locus_tag="VFR00_10000"
     CDS             959..1612
                     /locus_tag="VFR00_10000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HEU0159637.1"
                     /translation="MDVLLDLDGTLIDPGAGLIASIQYALDRLGKPVPPAAELGWMIG
                     PPLRASFAKLLGGMQATEAAIALYREHYFNRGMYDAVVYAGIPEALDRLQAAGCRLIV
                     ATAKPHHFARPILRHFALASRFAAIHGPELDGRRDAKAELLAHIVAEHNLATETAVMV
                     GDRAFDVHAAKANGIAGIGAAWGYGSRAELEAAGATAICATPDALAPLVLAVAGRRR"
     gene            1793..2203
                     /locus_tag="VFR00_10005"
     CDS             1793..2203
                     /locus_tag="VFR00_10005"
                     /inference="COORDINATES: protein motif:HMM:NF021073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HEU0159638.1"
                     /translation="MPDPADRRGPGERQMSPEEAAAFEGRVAELGDRLGRIKAQRDAD
                     AHADLDAEMRGRGMAYGMRMAAEMVGAVVVGGGIGWGLDWWLGTKPWLFLLFFLLGFA
                     AGVLNVVRGYERMQSEFMARTGGRIGHSVADDED"
BASE COUNT          368 a          731 c          772 g          373 t
ORIGIN      
        1 cgcgaccggc gtagcgctgc cgacgcgctg gctgccgccg acaccgccca ccgatcggcg
       61 gcccaggagc tgcgcgcggc gcaaacggcc gttgccgatg aacgcgaacg gcgcgctcgc
      121 actgaggcgc atctggagaa cgcacgccag cggcgcgccg aggaggcacg caagattcgg
      181 gagcaattgg gctgttcgcc cgaggactgc ctggcgctcg ccgacgtggc gccggcaacc
      241 ccgctgccga cgctagagac cgccgtcggc gggctcaatc gcctcaaggc cgagcgcgag
      301 cggctgggtg gcgtcaatct gcaggccgac gacgacctcg ccggcctcag ccagcagttc
      361 gagagcttgg acaaggaaaa gcgtgacgtc gaagaggcga tcgccaagct gcgtgccggc
      421 attgcccaaa tcaacaacga aggccggacg cggctgcagg cggccttcga cgccgtcaac
      481 gcccacttca agcagctttt catgaccctg ttcgggggcg gcgaggcgcg gctggagatg
      541 gtcgatgccg aggatccgct cgatggcggt ttggaaatta tcgccagacc gccgggcaag
      601 aagcccgcaa ccttatcgct gctctccggc ggcgagcagt cgctgacggc catggccctg
      661 attttcgcgg tgtttttgac caacccgtcg ccgatttgtg tgctcgacga ggtcgatgca
      721 ccgctcgatg acgccaacgt cgatcgcttc tgcaccttgt tggaaaaaat ggccgccgat
      781 acggcaacgc gtttcctggt gatcacgcat catcccatga ccatgtcgcg catgaatcgc
      841 ctgtttggcg tgaccatggt ggaaaagggt gtgagccaac tggtttcggt cgacctcgag
      901 acggcacaga agttcgtggt ggccgcctga gccgacaccc gcagagccac ccccccgcat
      961 ggacgtgctc ctcgatctcg acggcaccct gatcgatccc ggcgccggtc tgatcgcttc
     1021 gatccagtat gctctggacc ggctcggcaa gccggtgccg ccggccgccg agctcggctg
     1081 gatgatcggg ccgcccttgc gcgcctcctt tgcgaagctc ctgggcggaa tgcaggcgac
     1141 ggaagcggcc atcgcccttt atcgcgagca ctatttcaac cgcggcatgt acgacgcggt
     1201 tgtgtatgcg ggtattcccg aggctctcga tcgcctgcag gcggccggtt gccgcctcat
     1261 cgtggcgacg gccaaaccgc atcactttgc ccggccgatt ctgcgccatt tcgcgctcgc
     1321 gtcgcgcttt gccgccatcc atggccccga gctcgacggc aggcgggacg ccaaagccga
     1381 gttgctggcc cacatcgtgg ccgagcacaa cctcgcgacg gagaccgcgg tcatggtggg
     1441 cgatcgcgcc ttcgacgtgc atgccgccaa agcaaacggc atcgccggga tcggcgcggc
     1501 ctggggctat ggcagccggg ccgagctcga ggccgcgggc gcaacggcca tttgtgccac
     1561 acccgacgcc ctggcgccgc tggtcttggc cgtggccggc cgccgccgct gagggcccct
     1621 ctccttgccc cgcgcggggt cgaattgtcg catcagcggg taggcgcggc cgaccgacgg
     1681 gtgcggccgc gccatattct tgacactcgg gggaaccgct gctttaaggt gcgcgcgctt
     1741 tgatgggccg ccggggggag tcgggccgcc tcccaccgag gacataatcc tcatgccaga
     1801 tccagcggat cgtcgaggtc ctggtgaacg ccagatgagc cccgaagagg ctgctgcgtt
     1861 cgagggtagg gtagccgagc tcggtgacag gctcggccgc atcaaggcgc aacgggatgc
     1921 cgatgcgcat gccgatctcg atgctgaaat gcgcggccgc ggcatggcct acggcatgcg
     1981 catggcagcg gagatggtcg gcgcggtcgt ggtcggaggt ggcatcggtt ggggtttgga
     2041 ctggtggctc ggcaccaagc cctggctgtt cctgctcttt ttcctgttgg ggtttgcggc
     2101 cggcgtcctc aacgtggtga gaggctacga gcgcatgcag agcgaattca tggcacgcac
     2161 cggtggccgg ataggccaca gtgtggccga cgacgaggac tgaggagctt agcgtggcag
     2221 cgccgaatgc gggcacacag ggca
//
LOCUS       DASTMT010000430         5107 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_241171, whole genome shotgun sequence.
ACCESSION   DASTMT010000430 DASTMT010000000
VERSION     DASTMT010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 5107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5107
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_241171"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..598
                     /locus_tag="VFR00_10010"
     CDS             <1..598
                     /locus_tag="VFR00_10010"
                     /EC_number="2.1.3.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HEU0159639.1"
                     /translation="SGGARMQEGILSLMQMPRTTIAVQKLREARLPYLVVLTDPTTGG
                     VTASYAMLGDVHIAEPGALICFAGPRVIQQTIREQLPEGFQKAEYLLAHGMIDMVVHR
                     HKMRETLARLCRLLTATEKTVATRKKPLATKHEERGYLNGSAVDGQTIETLARGSAAM
                     EPEPVEDNERFEVRPKNARHDAETKPLPRPGSRDGARN"
     gene            706..2037
                     /locus_tag="VFR00_10015"
     CDS             706..2037
                     /locus_tag="VFR00_10015"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HEU0159640.1"
                     /translation="MRTSDQLLAELKALHPRRIDLSLGRISRLLAKLDNPQNRLPPVI
                     HVAGTNGKGSVSAYLKAMLQAAGRRVHVYTSPHLIRFHERIELAGAGGLAGPIGEAAL
                     IDCLSRVERSNAGDMVTFFEITTAAAFLAFAENPADALILEVGLGGRLDATNVVAQPA
                     LSIITPISLDHTEWLGETLPQIAREKAGILKRGVPAVISQQAFEALDAIAAQAEAIGA
                     PLTIWGRDYEAFEQRGRLVYQSAEQLLDLPLPALIGQHQIVNAGTAIAAAQHLEALGL
                     NAVPTLERGLIEARWPARMQRLTRGALAKSVDHGSELWLDGGHNPAGGAAIAHTLGEL
                     EERAPKPVGLILGMLDTKDAAGFLAPFAGLVRHIRTVPIAGTEAAQDAAALAKIASSM
                     GLAARPAADVASAVQELMAAEAAPIRILICGSLYLAGQVLAQEEGAQAQAN"
     gene            complement(2212..3372)
                     /gene="dapE"
                     /locus_tag="VFR00_10020"
     CDS             complement(2212..3372)
                     /gene="dapE"
                     /locus_tag="VFR00_10020"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HEU0159641.1"
                     /translation="MPIDRADPVQFAQALIRCNSVTPAEGGALTLLESVLKPAGFTCH
                     RLTMREAGTPDVENLYARLGSDGPHLCFAGHTDVVNAGDEARWSHPPFAAVIADGILY
                     GRGAVDMKGGIACFLAAALAYLKESGGLRRGSLSFLITGDEESVAINGTRKLLQWLRA
                     RDEVIDACLVGEPTSAASLGDEVKIGRRGYLNATVVVEGKQGHSAYGEKADNPIPKLA
                     RIIDRLASARLDDGTAHFQRTSLEPTIISVPNTATNVIPGTARVNFNIRYNDLHTRHS
                     LEAWVRDECQVAAQGAKFTVSFEGSGDVFLTEPGPFVQLMSSAVRAITGRTPALTTNG
                     GTSDARFIKDLCPVVELGLRNATAHQIDERVPVADLEALTAIYRRLIADFFR"
     gene            complement(3377..3847)
                     /locus_tag="VFR00_10025"
     CDS             complement(3377..3847)
                     /locus_tag="VFR00_10025"
                     /inference="COORDINATES: protein motif:HMM:NF017434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RimK/LysX family protein"
                     /protein_id="HEU0159642.1"
                     /translation="MQSPQNPFILGWEEWVALPELRLPAIKVKVDTGARTSALHAYFV
                     EPCGTGRKPMVRFGVHPIPRRSDVEVICTAAVVDRRHVASSNGERESRYVISTLLSIG
                     DRQWPIEVTLTNRNTMSYRMLLGRQAITDGILVDPASSFCQPRLRYALYTQQER"
     gene            4255..4767
                     /gene="def"
                     /locus_tag="VFR00_10030"
     CDS             4255..4767
                     /gene="def"
                     /locus_tag="VFR00_10030"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004120850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEU0159643.1"
                     /translation="MATLPIITLPNPLLRKRSAPVERIDAELGKLADDMLETMYAAPG
                     VGLAAVQVGVPRRLIVVDAAKDEDPPAPLVMVNPEIVAFGPELRMHEEGCLSIPDERI
                     EIERPGNVTVRFLDRHGELQELAAEGLLATVIQHEIDHLDGKLIIDYVSRLKRDIIIR
                     RFKKLAKSGA"
     gene            4782..>5107
                     /locus_tag="VFR00_10035"
     CDS             4782..>5107
                     /locus_tag="VFR00_10035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011995176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltransferase family protein"
                     /protein_id="HEU0159644.1"
                     /translation="MRIAFMGTPDFAVPTLQRLLAAGHEVAAVYTQPPRPAGRGMHER
                     SSPVQRQAMQRGLPVFSPKSLKQSSEQQAFAAHAIDAAVVVAYGLLLPKPVLQAPRHG
                     CFNLHAS"
BASE COUNT          894 a         1642 c         1691 g          880 t
ORIGIN      
        1 ttcgggcggc gcgcgcatgc aggagggcat tctttccctg atgcagatgc cgcgcacgac
       61 gattgcggtg cagaagttgc gcgaggcgcg cctgccgtat ctggtggtgc tgaccgatcc
      121 gacgaccggt ggcgttacgg cgtcctatgc gatgctgggc gacgtccaca ttgccgagcc
      181 cggtgcgctc atctgctttg ccgggccgcg cgtcattcag cagaccatcc gtgagcagct
      241 cccggaaggc tttcagaagg ccgagtacct gctggcgcac ggcatgatcg acatggtcgt
      301 gcatcgccac aaaatgcgcg aaaccttggc gcggctgtgc cgccttttga ccgcgacgga
      361 gaagacggtg gccacgcgca agaaaccgct cgccaccaaa catgaggaac gtggctatct
      421 caatggcagc gccgtggatg gtcaaaccat cgagacgctc gcgcgcggtt ccgccgccat
      481 ggaaccggag ccggtcgaag acaacgagcg gttcgaggtc cgtcccaaaa acgcgcgcca
      541 cgatgcagaa accaagccgc tgccgcgtcc cgggtcgcgt gacggagccc gcaactgatg
      601 cgcccaaggc gccatttgag gccccacggt tgagcctgcg caggccaacc gccgagcacc
      661 ggtgcctcgc cagtgccgca acggtgatgt gataagggcg cgtccatgcg cacgagcgat
      721 cagctattgg cagagttaaa ggcgctgcat ccccgacgca tcgatctgtc gttggggcgc
      781 atcagccgac tgttggcaaa gctcgacaat ccgcaaaacc gtttgccgcc ggtgatccat
      841 gttgccggca ccaacggcaa gggctcggtg tcggcttatt tgaaagccat gttgcaagcg
      901 gcgggtcggc gcgtgcacgt ctacacttct ccgcatctca tccgcttcca cgaacgcatt
      961 gaactcgccg gggccggtgg tttggccggc ccgatcggtg aagccgcact gatcgattgt
     1021 ctaagccggg tcgagcgcag caatgccggg gacatggtga ccttttttga gatcaccacg
     1081 gcagccgcct ttcttgcttt cgccgaaaat cccgcggacg ccctcattct ggaggtgggc
     1141 ttgggtgggc gtcttgatgc caccaacgtc gtggcgcagc ccgcgctcag tatcatcacc
     1201 cccatttcgc tggaccatac ggaatggttg ggcgaaacgc tgccgcagat cgctcgggaa
     1261 aaagccggca ttctgaagcg gggggtgccg gccgtgattt ctcagcaagc cttcgaggcc
     1321 ctcgatgcca ttgccgcgca ggcggaggca atcggtgcgc cgctgacgat ctgggggcgg
     1381 gactacgagg cattcgagca gcgcggtcgt ctcgtttatc aaagcgccga acagctgttg
     1441 gatctgccgc tacccgcgct gatcggacag catcagatcg tcaatgccgg taccgccatc
     1501 gccgccgctc aacatctcga agcccttggc ctcaatgcgg tgcccaccct cgagcgtggg
     1561 ctcatcgagg cgcgttggcc tgcacgcatg caacggctga cgcgtggagc gttggcaaag
     1621 agcgtcgacc acggctcgga gctgtggctc gatggcggac acaatcccgc cggcggcgcc
     1681 gcgatagccc acacgcttgg tgagcttgaa gaacgcgccc ccaaacccgt cggcttgatc
     1741 ttgggcatgc tcgacaccaa ggatgcggcg ggatttctcg ccccgtttgc cggactggtg
     1801 cgccatatcc gtaccgtccc catcgccgga acagaggcag cccaggatgc ggcggcgctc
     1861 gccaaaatcg cctcgagcat ggggcttgcg gctcgcccgg ccgccgatgt tgcgagcgcc
     1921 gtgcaggagc tcatggcggc cgaagcggct ccgatcagaa ttctaatctg cggctcgctg
     1981 tacctagccg gccaggtctt ggcacaggag gagggtgccc aggcgcaggc gaactagggg
     2041 tgcggcgatg ggcaccgaat aacgctgcgg ccgcgccaac ctgctggcga cgtgcaggtc
     2101 cgcggcggcg agcattgccc gccgcgccgc caagataagg acaaggaact tcgcggttgg
     2161 gcccgccttg tccccttcct gccagcctgc cagcttgggg cgggaacgtc gtcagcgaaa
     2221 gaagtcggcg attaagcgcc ggtagatggc ggtgagcgcc tcaagatcgg cgactggcac
     2281 acgctcatcg atctggtgtg ccgtggcatt gcgcaggccc aactccacca ccgggcagag
     2341 atctttgata aagcgtgcgt cagaggtgcc gccattggtg gtaagggccg gtgtgcggcc
     2401 ggtgatcgcg cggaccgccg aggacatcag ctgcacgaag gggccaggct cggtgaggaa
     2461 cacatcgcca ctgccctcaa aggaaacagt aaacttggca ccctgggcag cgacctggca
     2521 ctcatcgcgc acccaggcct caagcgagtg gcgggtgtgc agatcgttat agcggatgtt
     2581 gaagttcacc cgcgccgtac caggtatgac gttggtggcc gtgttcggca ccgagatgat
     2641 ggtgggttcc aagcttgtcc tctgaaagtg cgccgtgccg tcgtcgagcc gcgcgctggc
     2701 gagccggtcg atgatgcgcg cgagcttcgg aatgggattg tccgccttct cgccataggc
     2761 tgaatggccc tgcttgccct cgacgacgac cgtcgcgttg agatagccgc gccggccgat
     2821 tttgacctca tcgccaaggc tagccgcgct ggtcggttcc ccaaccagac aggcatcgat
     2881 cacctcgtcg cgtgcccgca gccattgcag cagcttgcgc gtgccgttga tggcgaccga
     2941 ctcctcgtcg ccggtgatga gaaacgacag ggagccgcgc cgcagcccgc cgctctcctt
     3001 caggtaggcc agcgccgcag ccaggaaaca ggcgatgcct cccttcatgt cgaccgcgcc
     3061 ccggccatag agaataccgt ccgcgatgac agcggcgaag gggggatggg accagcgggc
     3121 ctcgtcgccg gcattgacga catcggtatg cccggcaaag cacaggtgcg ggccatccga
     3181 gcccagccgc gcgtagagat tttccacgtc cggcgtgccc gcctcgcgca tggtcaggcg
     3241 atgacaggtg aagccagcgg gctttagcac gctttccagc agcgtcagcg caccgccctc
     3301 ggccggcgtt acgctgttac agcggatcaa tgcctgggcg aattgcaccg gatcggcgcg
     3361 atcgatgggc atggagttat cgttcctgct gggtgtagag agcatagcga aggcggggct
     3421 ggcagaagga cgaggccggg tcgacgagga tgccgtcggt gatcgcctgg cgcccaagca
     3481 gcatgcgata ggacatggta ttgcgattgg tcagcgttac ctcgatcggc cattgccggt
     3541 cgccgatgct gagcaacgtc gagatgacgt agcggctttc gcgctcgccg ttcgaactgg
     3601 cgacgtggcg ccgatccacc accgccgccg tgcagatgac ctcgacgtca ctgcggcgcg
     3661 gaatggggtg taccccgaac cggaccatgg gtttgcggcc ggtaccgcac ggctcgacga
     3721 agtaggcgtg cagggccgag gtgcgcgcgc cggtatcgac cttgaccttg atggcgggca
     3781 gcctcagctc tggcagcgcc acccattcct cccagccgag gatgaaaggg ttttgcgggg
     3841 attgcatgac acgctatccg gctcaacgct ttgcgtcctg tttgccagat cggctcgtcg
     3901 agagcaaacc gcccgatggc ggtgctcgcg gtggtccggc gctggctagg cgcagccgat
     3961 ttggcggcgg tggccggcgc ttgggcttat gcggcgctcc gttgcaagcc cgcgcgcgac
     4021 gcgggcgaag ggttgcgccg gcaagcaatc gcggcggccg cgcccggatc gttgcagggc
     4081 gtacccgcgc ccgccgctaa agccgcaacg cctcggtgcc tgaaccgtcc tcaggtctgg
     4141 tgttgcgggc cgagtaagct ggttcaagcg ccgccggccg gccggccagc gcgtcgccac
     4201 cggcgtcagg cctctgcaat tgacccggat ggggcggcgc cctatgtgag cagcatggcg
     4261 acactcccga tcatcacctt gcccaacccg ctcctgcgca agcgcagcgc ccccgtggaa
     4321 cgcatcgatg cggaactcgg caagctcgcg gacgacatgc tggagacgat gtatgccgca
     4381 ccaggggttg ggcttgccgc cgttcaggtg ggggtgccgc gccgcctgat cgtcgtcgat
     4441 gcggcaaagg acgaggatcc gccagcgcct ttggtcatgg tcaacccgga aatcgtcgcc
     4501 ttcggacccg agttgaggat gcacgaggag ggctgtctgt cgatccctga cgagcgcatc
     4561 gagatcgagc gccccggcaa cgtgacggtg cggttcctcg atcgtcacgg cgagctgcaa
     4621 gagctcgccg cggagggcct cttggccacc gtcattcagc acgagatcga ccatttagac
     4681 ggcaagctca tcatcgatta cgtgtccagg ctcaagcggg acatcatcat tcgccgcttc
     4741 aagaagttgg ccaagtccgg ggcctgacgc gggatccctc gatgcgcatc gcctttatgg
     4801 gcacccccga ttttgccgta ccaacgctgc agcggctgct ggcggcgggc cacgaggtcg
     4861 ccgctgttta tacccagccg ccgcggcccg ccgggcgcgg catgcacgag cgcagcagtc
     4921 ctgtgcagcg gcaggccatg caacgaggcc tgccggtctt cagtcccaaa agcctcaagc
     4981 agagctcgga acagcaggcc ttcgccgcgc acgcgatcga tgccgccgtg gtcgtcgcct
     5041 atgggctcct tctgcccaag ccggttttgc aagccccgcg tcacggctgt ttcaatttgc
     5101 acgcctc
//
LOCUS       DASTMT010000431         1670 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_4179006, whole genome shotgun sequence.
ACCESSION   DASTMT010000431 DASTMT010000000
VERSION     DASTMT010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 1670)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1670)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1670
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4179006"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(369..1076)
                     /locus_tag="VFR00_10040"
     CDS             complement(369..1076)
                     /locus_tag="VFR00_10040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016843589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018784 - (S)-2-haloacid dehalogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase type II"
                     /protein_id="HEU0159645.1"
                     /translation="MERPDILKREIKVLVFDQYGTVVDMQKGLREAVTPFLQKKGWSG
                     DANSFVTWWRRTHFENSMIDALCDRGHTPYRQIGHRAVSYVMDRCGLAYTQEEVRWLV
                     GQIETLKPFPDVLAALEKLRGAGYKLAILSNGDRDMLKAAGPHIGFAFEHVISVAEAG
                     YFKPHWRTYAKAEEILAEDRSSILFVANHAFDCIGAKAFGMRTAFIDRRRRPFGETPH
                     RPDLIVANFSELAATLV"
     gene            complement(1088..1669)
                     /locus_tag="VFR00_10045"
     CDS             complement(1088..1669)
                     /locus_tag="VFR00_10045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008964812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HEU0159646.1"
                     /translation="MFLAEKGISIPTHQVDLRGGEQFEPAFRAINEEGTVPVLVLDDG
                     TAITDAVAICCYFEDLQPEPALIGRTARQRALTIACNRQLERDGFYAVMEALRNSTPG
                     LKTRALPGPDDYEQIPALAERGRVRVNRFFARLDRRLAATAFVAGESYTIADITAFIT
                     VEFAQRAKLPVPPDCPHLARWLAKQSARPSAKA"
BASE COUNT          290 a          508 c          593 g          279 t
ORIGIN      
        1 tcgcgcggcg agcgcaaaaa acgcagccca gccgagatcg aacgcgagct cgcgtggacg
       61 gaggaggtct tgcataacct cgagggctgc atcctgcagc ttgagggcaa gcttttgacg
      121 gcgagcgggg cggcgccgcg ctcgcaggcc tagtcgccag gggcctagtc gccaggggca
      181 gccgcgggcc gacgcgccag caatggcgcc ggctgcgcgg gcccggctcg agggcggcgc
      241 caggcggcgg ggtgcgggct cgaaaagcgc acgcagggct gccgatggga gggcgcatcc
      301 cctgggggcg acagcgttcg gggtgcgccg gcccggcctt ggaccgacgg catcgttgcc
      361 aagcctggct agaccagggt ggccgccagc tcggaaaagt tggccacgat caggtcgggc
      421 cggtgcggtg tttcgccaaa cggccggcgg cggcggtcga tgaaggcggt gcgcatgccg
      481 aaggccttgg cgccgatgca gtcgaaggca tggttggcga cgaacagaat gctcgagcga
      541 tcctcggcca ggatctcctc ggccttggcg taggtccgcc agtgcggttt gaaataaccg
      601 gcctccgcga ccgagatgac atgctcgaaa gcaaagccga tgtgggggcc ggcggccttg
      661 agcatgtcgc ggtcgccgtt ggagaggatc gccagcttgt agccggcgcc gcgcagtttt
      721 tcgagcgcgg cgaggacgtc gggaaacggc ttcaaggtct cgatctggcc aaccaaccag
      781 cgcacttcct cctgcgtata ggccaagcca cagcgatcca tgacatagga gacggcgcga
      841 tggccgatct ggcggtaggg cgtgtggccg cggtcgcata gggcatcgat catcgagttt
      901 tcgaagtgcg tgcgccgcca ccaggtgacg aagctgttgg catcgccgct ccatcctttc
      961 ttttgcagaa acggcgtgac ggcctcgcgc aggcccttct gcatatcgac gacggtgccg
     1021 tattggtcaa agaccagcac cttgatttcg cgcttgagga tgtcggggcg ttccatgtcg
     1081 ttgtctctca tgccttggcg ctggggcgcg ccgattgctt ggcaagccag cgcgccaaat
     1141 gcgggcagtc cggcggcacc ggcagcttcg cccgctgggc gaactcgacg gttataaagg
     1201 cagtaatatc ggcgatcgta tagctctccc ccgccacaaa ggccgtggcg gccaggcgcc
     1261 gatcgaggcg cgcaaaaaac ctgttgacgc ggacgcggcc acgctcggcc aaggccggga
     1321 tctgttcgta gtcgtcgggg ccgggtaggg cacgggtttt caggccgggc gtagaattgc
     1381 gcaaggcctc catcaccgca taaaagccgt cgcgctcgag ctggcgattg caggcaatgg
     1441 tcagcgcccg ctggcgcgcc gttcgcccaa tcagcgccgg ctcgggctgc aagtcttcga
     1501 aatagcagca gatcgccacg gcgtcggtga tggcggtgcc gtcgtcgagt acgagcacgg
     1561 gtacggtgcc ctcctcattg atggcgcgaa aggccggctc aaattgttcg ccacccctga
     1621 ggtcgacctg gtgcgtgggt atgctgatgc ccttttccgc caaaaaaatg
//
LOCUS       DASTMT010000432         1967 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_3197040, whole genome shotgun sequence.
ACCESSION   DASTMT010000432 DASTMT010000000
VERSION     DASTMT010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 1967)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1967)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1967
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3197040"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            324..>1967
                     /locus_tag="VFR00_10050"
     CDS             324..>1967
                     /locus_tag="VFR00_10050"
                     /inference="COORDINATES: protein motif:HMM:NF014071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HEU0159647.1"
                     /translation="MMRVAFDIGGTFTDFVLEDVRAGHLKFHKLPTTPSDPAAAVLAG
                     LDALCKDAELAPAQISTILHATTVATNAILERKGAKVALVTTAGFRDVLIIGRQKRYD
                     TYDMYLAKPVPLLRRRDIYEVAERTLADGSVEAPLDLAELEQVLDRLEAQGYASVAVC
                     LLHAYARVEHERLIGERLARRNPRLAISLSSEVSPKFREYERASTVVANAYVKPIVNG
                     YVRRLTGALAQKGIGCDLFIMQSNGGLVPPELACEMPIRIVESGPAAGVLLCGVIGAE
                     EGLQRVLTFDMGGTTAKLGAIDGGEPAISPTFEVDQVRYRKGSGLPINVPALELLEIG
                     AGGGSIARTEMGLIKVGPESAGADPGPACYGRGGTQPTVTDANVVLGYINPANFNGGA
                     MRLEAQAAAEAIDRGLGAALGLDTGEAAWGIYTMANANMERAMRIVSVERGRDPRRYG
                     LVAFGGAGPLHAGRLARALGIPTIIVPWGAGVGSAIGLLEANTKLDQSLTRLVALRAG
                     AEAEISEIYGALADRMQADLKRLPRPSPPVFTRFAFLRYL"
BASE COUNT          300 a          674 c          659 g          334 t
ORIGIN      
        1 cgcagctgat cgaggtgttt gtcggcaagc cgcggcacgc ccgccgccgc cgccatctgg
       61 gcgagttcgg ctttgttcat gtggctccct tcgctcatgt ggctccctcc gcttggtgcg
      121 agcgggccga aactttgccc caactgtggg cggggcgaaa gggtggatcg cgggcgcgca
      181 ggattgccgc cgggcgggat tctggcgggc gcgctttggc tgttgccggc aagcagaggc
      241 tcgggctagc ttttcgcgcc ccggtggcgc gcaaatggta aggcgcaaat gctggcgcgc
      301 aacataagga ctagccgcac aagatgatgc gcgttgcctt tgacatcggt gggaccttca
      361 ccgacttcgt cctggaagat gtccgtgccg gtcacctcaa gttccacaag ctgccaacca
      421 cgcccagcga tccggcggcg gcggtgttgg cgggcctcga tgcgctgtgc aaagacgccg
      481 agttggcgcc ggcccagatc agcaccatcc tgcacgcgac cacggttgct accaatgcca
      541 tcctggagcg caaaggcgcc aaggtcgcgc tcgttaccac cgccggcttc cgcgacgtcc
      601 tgatcattgg tcgccagaag cgctacgaca cctacgacat gtacttggcc aagcccgtac
      661 cgctgctgcg ccggcgcgac atctacgagg tcgccgaacg cacgcttgcc gacggcagcg
      721 tcgaagcacc gctggatctt gccgagctcg agcaggtgct cgatcggctg gaggcgcaag
      781 gctatgcctc cgtcgccgtt tgcctgctgc atgcctatgc ccgggttgag cacgaacggc
      841 tcatcggcga gcgcttggcc cggcgcaacc cgcgacttgc catctcgctg tcctcggagg
      901 tctcgcccaa gttccgcgaa tacgagcgcg ccagcaccgt ggtcgccaat gcctacgtca
      961 agccgatcgt caacggctac gtgcgccgac tgaccggtgc cttggcgcaa aagggcatcg
     1021 gctgtgacct cttcatcatg cagtcgaacg gcggcctggt gccgccggag cttgcctgcg
     1081 agatgccgat ccgcatcgtc gaatcgggcc ccgccgccgg cgtcttgctg tgcggtgtga
     1141 tcggcgccga agagggattg cagcgcgtgc tcaccttcga catgggtggg acgaccgcca
     1201 agctcggcgc catcgacggc ggcgagccgg ccatctcgcc gaccttcgag gtcgatcagg
     1261 tgcgctatcg caagggcagc ggcttgccga tcaacgtgcc ggcgctcgaa ctgttggaaa
     1321 tcggcgccgg gggcggcagc atcgcccgca cggagatggg cctgatcaag gttggaccgg
     1381 agagcgccgg cgccgatccg gggccggctt gctacggccg cggcggcacg cagccgacgg
     1441 tcaccgacgc caacgtcgtc ctgggctata tcaaccccgc caacttcaat ggtggtgcca
     1501 tgcggctcga ggctcaggcc gccgccgagg cgatcgaccg cgggcttggt gcggccctcg
     1561 ggctcgacac cggcgaggcg gcctggggca tctacaccat ggcgaacgcc aacatggaac
     1621 gcgccatgcg gatcgtgtcg gtggagcgcg gacgcgatcc gcgccgctac ggtctggtcg
     1681 ccttcggtgg cgccggcccg ctgcatgcgg gccggctcgc ccgcgcgctg ggcatcccca
     1741 cgatcatcgt gccctggggt gccggtgtcg gctcggccat cggcttactc gaagccaata
     1801 ccaagctcga tcagtcgctg acgcgtcttg tcgccctgcg cgccggcgcc gaggccgaga
     1861 tcagcgagat ctacggcgcc ctcgcagacc gcatgcaggc ggatctgaag cgtctgcccc
     1921 gtccgtcgcc gcccgtcttc acgcgctttg ccttcctgcg ctacctg
//
LOCUS       DASTMT010000433         2419 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_5702084, whole genome shotgun sequence.
ACCESSION   DASTMT010000433 DASTMT010000000
VERSION     DASTMT010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2419)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2419)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2419
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5702084"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            14..364
                     /locus_tag="VFR00_10055"
     CDS             14..364
                     /locus_tag="VFR00_10055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0159648.1"
                     /translation="MAKAQRSIFMVSMDVDPDKEALFNEVYDQEHVPNLLKVPGVHAA
                     RRAKAEPAVRLNIGGEEKDIAHVGATYCAIYEIDGPHVLTSPEWARAGEAGRWPSQVR
                     PFTRNRHHAIYKVL"
     gene            complement(533..1129)
                     /locus_tag="VFR00_10060"
     CDS             complement(533..1129)
                     /locus_tag="VFR00_10060"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HEU0159649.1"
                     /translation="MYFNKRQDQRLDVPRLERRPLPREDDCKVELNLPKVRPRSLIEA
                     SVTVTGDLWSEGDLEIDGHLCGNINCRQLIVGKNAAITGVIVAQEAVIRGKVTGIIRA
                     VRVLLQDSARVESEIIYRTLSVDEGASFEGRARPRPNPLAEEPGLSPAVDLRQKVTQV
                     EAPCANGAAGSSQPTQTGATPANPDGQRQLAHASPASR"
     gene            1542..1931
                     /locus_tag="VFR00_10065"
     CDS             1542..1931
                     /locus_tag="VFR00_10065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0159650.1"
                     /translation="MAEVKNPAQAKLRDWPMFQGAAGAAKRRRSDGRPLPKIAELVVN
                     HLDGTAEEKLAWLLAQKTAGKLDGDDPDEVAEAQTLLAMLARLAHSKGRKQHLSDLLD
                     EGLKASFPASDPIAVGRFTGAEVSSTS"
BASE COUNT          435 a          804 c          791 g          389 t
ORIGIN      
        1 caggagacca ggcatggcaa aggctcagcg ctccattttc atggtcagca tggacgtcga
       61 tccggacaaa gaagccctgt tcaacgaggt ttatgatcag gagcacgtgc ccaatctgct
      121 caaggtgccg ggcgtgcacg cggccaggcg tgccaaggcc gagccagccg tccggctcaa
      181 catcggcggc gaagaaaaag acattgccca cgtgggggcg acctactgcg ccatctacga
      241 gattgatgga ccgcacgtgc tcaccagtcc cgaatgggcg cgcgccggcg aagcgggacg
      301 ctggcccagc caagtgcggc cgttcacgcg caaccggcat cacgccatct ataaggtgct
      361 ctaggctcct tgcgcgcatc gctctgcgcc tggcaacggg cgcacgctga cgcagccgac
      421 cccgtgccca gtgcagtgcc gccgacatcg tcctgaccag gggaccgcgc cggcatcatc
      481 gccgccgcaa tcgttggcgg cgaccggtgc gcgccagcgt caggcctggg ccttagcggc
      541 tggcggggct cgcgtgcgcc aactgacgct gcccatctgg gttcgccggc gtcgcgccgg
      601 tctgcgtcgg ctggctgctg ccggctgcgc cgttggcgca gggggcctcg acctgggtca
      661 ccttctggcg cagatcgacc gcgggcgaga gccccggttc ctcggcgaga gggtttggcc
      721 gcggacgtgc gcgaccctcg aagcttgcgc cctcatcgac ggacagggtc cgatagatga
      781 tttcgctttc cacccgcgcg ctgtcctgca acagcacgcg caccgcccgg atgatgccgg
      841 tgaccttgcc gcgaataacg gcctcttgcg cgacgatgac gccggttatg gcggcgttct
      901 tgccgacgat caattgccga cagttgatgt taccgcacag gtggccgtcg atctcgaggt
      961 cgccttcgct ccacagatca ccggtcacgg tcaccgacgc ctcgatcagg gagcgcggcc
     1021 gcaccttggg aaggttcaac tccaccttgc aatcgtcctc ccgcggcaag gggcgccgtt
     1081 cgaggcgcgg gacgtccagg cgttggtctt gccgtttgtt gaaatacatt tttcacaccc
     1141 tgcctgcccg ctttcatcga gggatcgtgg gtcgagaatg tgcagtgccc gccagggtta
     1201 gccgcgccaa gctgcccacc gagggggcga agcgaacccc ggcgaacggg atcggtcccg
     1261 gcaagccgac gctgtccgac agctggtcgc aatgaggtca gttagtagac ggcaaaggcc
     1321 aggcaaacac ctgcgtggag gctgtatttt gcgcaaaatc attgtgtagc ggttgcgccc
     1381 gccggctctc gccgccgcgc catgccctgc cgccgtggcg cgctttgtgc ggccgcgaaa
     1441 tctgcctggg ggcgaccata tccgctgcgg caaagatgcg ataaagtcgg gcgaggtcgg
     1501 ataagcggct cacccatagt tcggtcgttg gagtttttgt tgtggccgaa gtcaagaacc
     1561 ccgcgcaggc gaagcttcgc gactggccga tgtttcaagg cgccgcgggc gcggccaagc
     1621 gcaggagatc cgacggacgg ccgctgccga aaattgccga gcttgtggtc aaccaccttg
     1681 atggcacggc cgaagaaaag ctggcctggc tgctggcaca aaaaacggcg gggaagctcg
     1741 acggcgacga ccccgatgag gtcgccgagg cccagacgct gttagccatg ctggcgcgcc
     1801 ttgcgcacag caagggccgc aagcaacact tgagcgactt gctcgacgag ggcttgaaag
     1861 cgagctttcc ggccagcgat ccaattgcgg ttggacggtt caccggggcc gaggtctcgt
     1921 ccacctctta agatcgcgac ggatgcggcg cagggtggtg cggggtttgg cgcactgccc
     1981 cgtgctgtga tccggcacgc cggcgccggc cgcccgcgcg ttgcacgtct ttccaggcaa
     2041 tagccatagc tgaggccata gccgcggcga aagccgaggc ggccaagccg caccaggccg
     2101 acagaaagat cgagcgcacc aacggcccgc accggcaggc aggatggcac tgcccaggcc
     2161 tacttgaccc gatcgcatct cgccgatcac ggccagcctc cggccaatgg tcggcaatag
     2221 ccgcatcgcc gccaaaatat ccgccacggc attcgctcac cgagcttgcc gacgtgcgca
     2281 agacgcgacg gccgcagcac ctgggcgaca tcctttgagc gcggctcgac gccggcaaaa
     2341 cctgtacggt ggcaggcccc gtcgaccgga ccgagccgaa cacgatgacg cccagcacca
     2401 tgctttctcg caacccgac
//
LOCUS       DASTMT010000434         3472 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_1231601, whole genome shotgun sequence.
ACCESSION   DASTMT010000434 DASTMT010000000
VERSION     DASTMT010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3472)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3472)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3472
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1231601"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..223)
                     /locus_tag="VFR00_10070"
     CDS             complement(<1..223)
                     /locus_tag="VFR00_10070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEU0159651.1"
                     /translation="MGVVVPFEESREPGEGLQGLLEIASEDMERVNRIILDRAVSEVE
                     LIPQIAHHLIDSGGKRLRPMLTIAAAKLCG"
     gene            367..606
                     /locus_tag="VFR00_10075"
     CDS             367..606
                     /locus_tag="VFR00_10075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007597676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HEU0159652.1"
                     /translation="MREMLRSNDAVLLSYAESVLQEQGIASLIADQHISVIEGSIGAF
                     PRRLLVREEEWSAAHRALREAGLEAWLIADGEAHA"
     gene            603..1400
                     /locus_tag="VFR00_10080"
     CDS             603..1400
                     /locus_tag="VFR00_10080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEU0159653.1"
                     /translation="MTGAGDRAAGSTEDAFLGGRLRILQPAAGYRAGLDAVLLAAAAP
                     LRMGETEQVLDVGAGVGVVGLAVARRVPDARVTMIEREMAAADLCRANIERNGLLERA
                     RVLAGDLGRPLGEQPALQRLAASFDHVLANPPYHVEGTASLAPVPAKAMANAMPADGL
                     GHWLRFMAAMARPGGTATMIHRADRLSHVLSAFAGRFGALLVFPLFARPGAAAQRILV
                     QGIKGSRAPLQLRPGLILHDAGHGFRPEVDAILRAGHALELGRTGGG"
     gene            1535..2377
                     /locus_tag="VFR00_10085"
     CDS             1535..2377
                     /locus_tag="VFR00_10085"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HEU0159654.1"
                     /translation="MLFFNRGPIVPVLRFTGAIGMVTPLRPGLALASLAGAIEKAFTA
                     SKLPSVAITVNSPGGSPVQSHLIFNRIRQLAQEKDKRVYVFCEDIAASGGYFLALAGD
                     EIYADPSSIVGSIGVISAGFGLDKFIERFGIERRVHAAGKDKGALDPFRPERADDVQR
                     LEELQREVHETFIGIVKARRADRLKGPEEEMFSGSFWSARKAVDYGLIDGITDLRSKM
                     RALHGDKVRLKAVPMAPTGLLSRLRRMPGLGDGGSGFAFPTSLADDVISAVEARALWA
                     RFGL"
     gene            2399..2611
                     /locus_tag="VFR00_10090"
     CDS             2399..2611
                     /locus_tag="VFR00_10090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0159655.1"
                     /translation="MPQLIVIALIGAGFYAAYRWMRQASDAVTAELSRTEDELKRRTA
                     GGRIEKDLGRLEYDPATGVYRPQKRG"
     gene            2738..>3472
                     /locus_tag="VFR00_10095"
     CDS             2738..>3472
                     /locus_tag="VFR00_10095"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HEU0159656.1"
                     /translation="MQKKAATPAAGKIAKAPPGLQPTASFQDLILTLQQYWGEQGCAI
                     LQPYDMEVGAGTFHPATTLRALGPKPWNAAYVQPSRRPKDGRYGENPNRLQHYYQFQV
                     IMKPSPDDFIDLYLASLARIGIDPNVHDIRFVEDDWESPTLGAWGLGWEVWCDGMEVT
                     QFTYFQQVGGFDCNPVAGEITYGLERLAMYVQGVDRVFDLNFNGRHDARKLSYGDVFL
                     QAEQEYSRFNFEYADTEILLQHFRDAE"
BASE COUNT          584 a         1131 c         1118 g          639 t
ORIGIN      
        1 agccgcagag cttggcggcg gcaatggtga gcatgggtcg cagccgtttg ccacccgagt
       61 cgatgagatg atgcgctatc tgagggatca gctcgacctc tgatacggcc cggtcgagaa
      121 tgatgcgatt gacacgctcc atgtcctcgc tggcgatctc gagcagccct tgcaagccct
      181 cgccaggctc gcgcgattct tcgaatggga cgacgacacc caaggtgtgc tattcctcgc
      241 ttggccaatg cccgttcccg tataacgggc agccatcaag aacagtcaag cgtgttttcc
      301 gcgacagagc gcccgatagg ttcgcagcgg ctcgccttgg cgcggcgcgc catcgggagc
      361 tccaggatgc gtgaaatgct gcgcagcaac gatgccgtgc tcctcagtta tgctgaatca
      421 gtgctgcagg aacagggcat cgccagcctg attgccgatc aacacatcag cgtgatcgaa
      481 ggctcaatcg gcgctttccc acgtcgcctg ctggtccgcg aagaggaatg gtctgccgcc
      541 caccgcgcgt tgcgcgaggc ggggctggaa gcctggttaa tcgctgatgg ggaagcgcac
      601 gcgtgacggg cgccggcgac agggcggcgg gcagcaccga ggatgctttt ttaggcggac
      661 gcttgcgcat tctgcagccg gccgccggct atcgcgccgg gctcgatgcc gtgttgctcg
      721 cagcggccgc gccgctgcgg atgggagaga ccgaacaagt cctcgacgtc ggcgccggcg
      781 ttggcgtggt cgggcttgcc gtggcgcgcc gcgtccccga cgcacgcgtg acgatgatcg
      841 aacgcgagat ggcggcggcg gacctctgcc gtgccaacat cgaacgcaat ggcctgctcg
      901 agcgcgcccg ggttcttgcc ggtgacctcg gccgaccgct tggggagcag cccgcgctgc
      961 agcggctcgc cgcgagcttc gaccacgtcc tggccaaccc gccttatcac gtcgagggta
     1021 ccgccagcct tgcccctgtg ccggccaagg ccatggccaa cgccatgccg gccgacggcc
     1081 ttgggcattg gctgcggttc atggccgcaa tggcgcggcc cggcggtacg gccaccatga
     1141 ttcaccgggc ggatcgcctg agccatgttc tctctgcctt tgccggccgc ttcggcgcct
     1201 tgctggtctt tcccctgttt gcccgccccg gcgcggcggc gcagcggatc ctagttcagg
     1261 gcatcaaggg gagccgcgcg cccctgcaat tgcgccctgg cctcatcttg cacgacgccg
     1321 gacacggctt tcgtcccgaa gtcgacgcca tcttgcgcgc gggacacgcc ctcgagctgg
     1381 gccgcaccgg cggcggttga gagcgcctgt cgcggcagtg acaaagttga tatctagcgg
     1441 ttggccgggc tttggtgcta ggcacggggc gcagccggcg ccgcgacctc gcagccgtcc
     1501 ttgcgtgccg cccattcctt tcctgaggca gccgatgctc ttctttaacc gtggcccgat
     1561 tgttccggtt ttgcgcttta cgggggcgat cggcatggtg acgccgctga gacccggtct
     1621 cgccttggca tcgcttgccg gcgccatcga aaaggccttc acggcctcga aactgccaag
     1681 cgtggcgatc accgtcaatt cgcccggtgg ctccccggtg caatcgcact tgatcttcaa
     1741 tcgtattcgc cagctcgccc aagaaaaaga caagcgtgtt tacgtgtttt gtgaggacat
     1801 tgccgcctcc ggcggctact tcttggcgct cgccggcgac gaaatctacg ctgacccctc
     1861 ctcgatcgtg ggttcgatcg gcgtgatttc ggcgggcttc ggtctcgaca agttcatcga
     1921 gcgtttcggc atcgagcggc gcgtgcacgc agccggcaag gacaaaggtg cgctcgaccc
     1981 gttccgaccc gaacgcgccg acgatgtgca gcggctcgaa gagttgcagc gtgaggtgca
     2041 cgagaccttc atcggtatcg tcaaggcacg ccgcgccgat cggctcaagg gaccggagga
     2101 agagatgttc tcgggcagct tctggtccgc gcgcaaggcc gtggactatg gcctgatcga
     2161 cggcatcacc gacctgcgca gcaaaatgcg cgccctacac ggcgacaagg tgcgattgaa
     2221 ggcggtgccg atggcaccga ccggcttgct cagtcgcctg cgccgcatgc ccggcctcgg
     2281 cgacggcggc agcggatttg ctttcccgac ctcgttggcc gatgatgtga tctcggccgt
     2341 cgaggcgcgt gcgctgtggg cgcgcttcgg cctttaggtg tcagcgggag gcaatcgcat
     2401 gccccagctc atcgtcattg ctttgatcgg tgccggcttt tacgccgcct accgctggat
     2461 gcggcaggcc tccgacgccg tcacggccga gttgagccgg acggaagacg agctcaagcg
     2521 ccggaccgcg ggcggacgca tcgaaaagga cttgggtcgg ctcgagtacg atcccgcaac
     2581 cggcgtctat cggccgcaaa agcgcggctg agggagcaaa gctgaaccct cgcgcgtgcc
     2641 gcagcagccg cgcctgcccg tcggcgttgg aattgtggtg accgatcgtc ccatttgcgt
     2701 atagtgcgcg cgccgcagcc ccattcagac gaactccatg caaaagaaag ccgccacccc
     2761 ggcagccgga aagattgcca aggccccgcc cggcctgcag cccacggcat cctttcaaga
     2821 cctgattttg accctgcagc agtactgggg cgagcagggc tgcgccatcc tgcagcccta
     2881 cgacatggag gtgggcgcag gcaccttcca tcccgcaaca acgctgcgcg ccttgggacc
     2941 aaagccctgg aacgccgcct acgtgcagcc ctcgcgccga cccaaggacg ggcgctacgg
     3001 cgagaaccct aatcgcctgc agcactacta tcaatttcag gtcatcatga agccgtcgcc
     3061 cgacgatttc atcgatcttt atttggcgag cctcgcccgc attggcatcg atcccaacgt
     3121 gcatgacatt cgctttgtcg aggacgattg ggagagcccg acgctcggcg cctggggact
     3181 gggctgggag gtatggtgcg atggcatgga ggtgacccag ttcacctact ttcaacaggt
     3241 cggcggcttc gactgcaatc cggtcgcagg cgagatcacc tacggcctcg agcggctcgc
     3301 catgtacgtg cagggtgtcg accgggtctt cgacctcaat ttcaatgggc gacatgatgc
     3361 acgcaagctg agctatggcg atgtgttctt gcaagccgag caggagtact cgcgcttcaa
     3421 ttttgagtac gccgacacgg aaatcctgct gcagcatttc cgcgatgccg ag
//
LOCUS       DASTMT010000435         3603 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_2537981, whole genome shotgun sequence.
ACCESSION   DASTMT010000435 DASTMT010000000
VERSION     DASTMT010000435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3603)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3603
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2537981"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            34..981
                     /gene="trbG"
                     /locus_tag="VFR00_10100"
     CDS             34..981
                     /gene="trbG"
                     /locus_tag="VFR00_10100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009291 - unidirectional conjugation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein TrbG"
                     /protein_id="HEU0159657.1"
                     /translation="MEVPEIPLLDSPAFTAATREPDPKPETTYVEVPKPLPLPGQLKP
                     VPPAAKRAEGAEVAPLPPPKERIAAAQRGARVEPSKDGYINAIQVYPYARGALYQLYA
                     AVNQVTDIGLEPGERLVSVSAGDTVRWIVGDTSSGEGKDAVVHILIKPTAADLDTNLV
                     ITTDRRTYHLEMHATAATYMASLTWTYPAGELLTLKRQRAEAPNAAAAVAEPGVNLEE
                     LRFRYRLEGDAPWKPRQVFDDGAKVYIQFPPGLAQGEAPPLFVLGPDGKSALVNYRVR
                     GTTYIVDRLFAAAELRLGTAPQRVVRIIRTDAVWREVQQ"
     gene            978..2204
                     /locus_tag="VFR00_10105"
     CDS             978..2204
                     /locus_tag="VFR00_10105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008871929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbI/VirB10 family protein"
                     /protein_id="HEU0159658.1"
                     /translation="MTIEAPAPDSGKVAPETLTLRANPQPVTRINRRVLIGIAAVVMF
                     VLSGLVLVALKPPSLRSGERPELFNVEHKPITDALAKLPASYGDVALAKPADGPQLPA
                     GVPRLALSLDGEEDGNAADNGQLTRLLGQARQSPVFFRLLAPATKAGGDLVVPPRAEG
                     AAPRLGENGLTPLASTPSTGEQGSSNPPASEPMRKLAFLRAGPDKDIYNPHALERPAS
                     PFQLMAGTVIAASLISGLNSDLPGFAIAQVTEHVYDSVSGRTLLIPQGSRLLGRYDNV
                     TAFGQKRALVVWQRLILPDGSSVLIDNLPATDTSGYAGLADSIDLHTFQLLKGVALAT
                     VLGVGSELALGSSDNNLVRALQAATQTSGNRVGQRLIERLIDVGPTLTVRPGWPLRVI
                     VHKDIVLKPYATPPNP"
     gene            2217..2540
                     /locus_tag="VFR00_10110"
     CDS             2217..2540
                     /locus_tag="VFR00_10110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF736 domain-containing protein"
                     /protein_id="HEU0159659.1"
                     /translation="MPVIGTFTHTQDGYTGSITTLALNAEVVILPNAGKQGHNAPDFL
                     IMAGPVEVGAAWRRISHSTGKPYLRVKIDDPLLPEPIWGALLGNGEDGVARLLWRREF
                     EDDGA"
     gene            2631..>3603
                     /locus_tag="VFR00_10115"
     CDS             2631..>3603
                     /locus_tag="VFR00_10115"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0159660.1"
                     /translation="MTRSRRIGCVLLPFAAGYYLSYLFRTINALIAADLTAELGLTAA
                     DLGLLTSVYFLVFAAAQIPCGILLDRHGPRTLLSGLLLIASAGALLFALADTLLGLLL
                     GRVLIGVGVALALMGGFKAIVLWFPPERLALTNGWFVMLGALGAVTATAPAELLIAAV
                     GWRGLFAVLGALSALAALLIVGIVPDQARKPAPSKRGQPALLTIYCERRFWRLAPLSA
                     LGIGTSWSLQGLWAAPWLRDVDGLDRPAVVAILSVMAIVVSASGLGLGVAANHIRRLG
                     GKTEHVLLGTVGLSLSAQLALLFELPVPRTLAWSAIAAAGAATVLSYA"
BASE COUNT          562 a         1341 c         1094 g          606 t
ORIGIN      
        1 ctcgccgttg ccaccggcgc ctgcgctcgg cccatcgagg tcccggaaat ccctctgctc
       61 gacagcccgg ccttcaccgc ggcaacgcgc gagcccgatc ccaaacccga gaccacctac
      121 gtcgaggtcc ccaaaccctt gccgctgccg ggtcagctca aaccggttcc gcctgcggca
      181 aaacgcgccg aaggcgccga ggtcgcgccg cttcctccac caaaggagcg catcgccgcc
      241 gcccagcgcg gcgcccgcgt cgagcccagc aaggatggct atatcaatgc tatccaggtc
      301 tatccctacg cccgcggcgc cctctatcaa ctctatgccg ccgtcaatca ggtcaccgac
      361 atcggcttgg agcccggcga aaggctcgtc tccgtctccg caggcgatac cgtgcgctgg
      421 atcgtgggcg acaccagcag cggcgagggc aaggacgccg tcgtccacat cctgatcaag
      481 ccgaccgccg ccgacctcga caccaacctc gtcatcacca ccgatcgccg cacctaccac
      541 ctggagatgc acgcaaccgc tgcgacctac atggcctccc tcacctggac ctatcccgcg
      601 ggcgaattgc tgacgctgaa acgccagcgg gcggaggctc caaacgcggc ggcggccgtt
      661 gccgagcccg gcgtcaatct cgaagagctg cgctttcgct accggctcga aggcgacgca
      721 ccctggaagc cgcggcaggt gttcgacgac ggggccaagg tctacatcca atttcctccc
      781 ggcctggcgc aaggcgaagc cccgcccctg ttcgtgctgg gccccgacgg caaaagcgcg
      841 ctcgtcaact atcgcgtgcg cggcaccacc tacatcgtcg atcgcctgtt cgccgccgcc
      901 gagctgcggc tcggcaccgc gccgcagcgc gtcgtgcgca tcatccgcac cgatgccgtg
      961 tggcgggagg tgcaacaatg acgatcgagg cccccgcgcc cgacagcggc aaggtcgccc
     1021 ccgagacgct caccttgcgc gcaaacccgc agcccgtcac gcgcatcaac cggcgcgtct
     1081 tgatcggcat agccgccgtc gtcatgtttg tgctgtcggg attggttctc gtcgcattaa
     1141 agccgccgag cctgcgctcg ggcgagcggc ccgagctttt caatgtcgaa cacaagccca
     1201 tcaccgatgc actcgccaag ctgccggcga gctatgggga cgtagccctg gccaaaccgg
     1261 cggacggacc gcaactgccg gcgggtgtgc ccagactggc gctgtcgctc gacggcgagg
     1321 aggacggcaa tgccgccgac aatgggcaac tcacgcgctt gctcggccag gcccggcagt
     1381 ccccggtgtt ctttcggctg ctggcgccgg caaccaaggc cggcggcgac ctcgtcgtgc
     1441 cgccgcgcgc agagggcgcg gcgccccgcc tcggagagaa tgggctcaca ccgctggcat
     1501 cgacgccgag tactggggag caggggtcca gcaacccgcc ggcaagcgag cctatgcgca
     1561 agctcgcctt cctgcgcgcc ggaccggaca aggacatcta caatccgcac gcgctggagc
     1621 gcccggcctc gcccttccag ctgatggccg gcaccgtcat cgccgccagc ctgatcagcg
     1681 gtctcaactc cgaccttccc ggctttgcca tcgcccaggt gacggaacac gtctacgaca
     1741 gcgtttccgg gcgcaccctc ttgatccccc agggctcgcg tctcctgggc cgctatgaca
     1801 acgtcaccgc ctttggccag aagcgcgcgc tcgtcgtctg gcagcgcctg atcctgcccg
     1861 acggcagctc ggtcctgatc gacaacctgc cggccaccga caccagcggc tatgcggggc
     1921 tcgccgacag catcgacctt cacaccttcc aactcctgaa aggcgtggcg ctcgcgaccg
     1981 tgttgggcgt gggcagcgag ctcgccttgg ggtcgtccga caataacctg gtgcgggcgc
     2041 tgcaggcggc aacccaaacc agcggcaacc gcgtcggcca gcgcctgatc gaacgcctga
     2101 tcgatgtcgg ccccacgctc accgtgcgtc cgggctggcc cctgcgcgtg atcgtgcaca
     2161 aggacattgt tctcaagccc tacgccaccc cgccaaaccc ctaaaggagg agccccatgc
     2221 ccgtgattgg cacgtttacg cacacgcaag atggctacac cggcagcatc accacgcttg
     2281 ccctcaacgc cgaggttgtc atcctcccca atgccggcaa gcagggccac aacgccccgg
     2341 attttctgat catggcgggg ccagtcgaag tcggcgcggc ctggcgccgg atcagccaca
     2401 gcaccggcaa gccttacctg cgcgtcaaaa tcgacgatcc gctgctgccc gagccgatct
     2461 ggggcgcctt gcttggaaac ggcgaggatg gcgtcgcacg cctgctgtgg cgacgtgagt
     2521 tcgaagacga cggcgcttga gcctgacggt ctgtgcttcc ctgctccttg ggcgctcctt
     2581 gggcgcggga gaaaccaatt tagcaaacca aaatttttgg gagagagccc gtgacccgtt
     2641 cacgccggat cggttgcgtt ctgctgcctt tcgcagccgg ctattatttg tcttacctct
     2701 ttcgcaccat caacgccctc atcgccgccg atttgacggc ggagctcggc ctcaccgcgg
     2761 cggacctcgg gctgctgact tccgtctatt ttctggtgtt tgccgccgcc cagatcccct
     2821 gcggcatcct gctcgaccgc cacgggcccc gcacactcct gagcggactt ctcctcatcg
     2881 ccagcgccgg cgccctcctg tttgcgttgg ccgacacgct cttgggcctt ctgttgggcc
     2941 gggtgctgat cggggttggc gtggcgctgg ccctgatggg cggcttcaag gccatcgtgc
     3001 tgtggtttcc gcccgagcgg ttagcactca ccaacggctg gttcgtcatg ctgggcgccc
     3061 tgggcgccgt cacggccacg gcaccggccg agctcctgat cgccgccgtc ggctggcgcg
     3121 gcctgtttgc cgtcctcggc gccttgagcg cgctcgccgc cctcctcatc gtcgggatcg
     3181 tgcccgacca ggctcgcaag cccgcgccgt ccaaacgcgg gcagcccgcc cttcttacga
     3241 tttattgtga gcggcgcttt tggcgcctgg ccccgctctc cgccctcggc atcggtacct
     3301 cctggtcgct gcaagggcta tgggccgcac cttggctccg ggacgtcgat ggcctcgatc
     3361 gcccagccgt ggtcgccatc ctgagcgtca tggccatcgt cgtcagcgcc agcggcttgg
     3421 ggctgggggt agcggcaaat catatccgcc gcctcggcgg caagaccgag cacgtgctgt
     3481 taggcaccgt cggcttgtcg ctctcagccc agctcgccct gctgttcgag ctgccggtgc
     3541 cccgaactct cgcctggagc gcgattgctg ccgccggcgc cgccactgtg ctgagctacg
     3601 cca
//
LOCUS       DASTMT010000436        12048 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_905557, whole genome shotgun sequence.
ACCESSION   DASTMT010000436 DASTMT010000000
VERSION     DASTMT010000436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 12048)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12048)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12048
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_905557"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..729)
                     /locus_tag="VFR00_10120"
     CDS             complement(<1..729)
                     /locus_tag="VFR00_10120"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020811982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEU0159661.1"
                     /translation="MAIAFVFPGQGSQVVGMGAELAATYAPARAVFAEIDAALGQKLS
                     ELMFAGPLEQLTLTENAQPALMAVSLAVMRVLEVEKGFRLADRVSCVAGHSLGEYSAL
                     AAAGALSLADAARLLKLRGQAMQAAVPVGQGAMAALLGVGKEVAEKLAAEAAESEICQ
                     LANDNEPTQAVISGSKAAIDRAGKLAKAHGVRRFMPLNVSAPFHSALMRPAAEAMAQA
                     LAQVKISSPQVPVVANVLAAPISDA"
     gene            1027..1611
                     /gene="rpsF"
                     /locus_tag="VFR00_10125"
     CDS             1027..1611
                     /gene="rpsF"
                     /locus_tag="VFR00_10125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014495806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEU0159662.1"
                     /translation="MPLYEHVFLARQDISQTQVEALTKEYSQVIEEGGGKVGKTEYWG
                     LKTIAFKIKKNRKAHYSLMNINAPPAAVAEMERRMGLSSDVIRFMTVRVDAHETEPSV
                     QMRKGDRDDRRDGDRGGFRGDRGGFRGGDRGGFRGGFRGGGGGGFRREGGEGGFRREG
                     GGEGGGFRREGGDGDRPRGPRPPRDEAESTSAEE"
     gene            1617..1865
                     /gene="rpsR"
                     /locus_tag="VFR00_10130"
     CDS             1617..1865
                     /gene="rpsR"
                     /locus_tag="VFR00_10130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006203718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEU0159663.1"
                     /translation="MSEVTTIPARRPFHRRRKTCPFSGDNAPKIDYKDMRLLGRYVSE
                     RGKIVPSRITAVSAKKQRELARAIKRARFIGLLPYLIK"
     gene            1960..2904
                     /locus_tag="VFR00_10135"
     CDS             1960..2904
                     /locus_tag="VFR00_10135"
                     /inference="COORDINATES: protein motif:HMM:NF021506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2232 domain-containing protein"
                     /protein_id="HEU0159664.1"
                     /translation="MPIGLLTGVGSGLASALLFYSATRGSPLLSSFLLLLTPLPALIA
                     GLGWGWLPALLGGVVASAAMLAIASLPFAVGYFLALGGPSALSAYLAYLNRTDAEGVV
                     QWYPPGRLLAALALYGGALPILVLPMLGGSYEILREPMAEYFRRFSSRTASDLNMPPL
                     NEAQIESLTEFLIAVLPAALSAYWLILFTINLYLAARVARASDRLGRDWPDLAALAYP
                     PGFPLIAVAAFAASFAPGVLGLLGTSFSGALLVAYLIAGLALMHFLARGRAPWLLWLV
                     YGGLFLLGPYAALGLTVAGLLEPVINLKRRLGAPPSST"
     gene            2974..3573
                     /gene="rplI"
                     /locus_tag="VFR00_10140"
     CDS             2974..3573
                     /gene="rplI"
                     /locus_tag="VFR00_10140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008551838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEU0159665.1"
                     /translation="MQIILLERIGRLGQMGDVVNVKDGYARNFLLPQKKALRATAENL
                     ARFEKERSQLEARDLELKKEAEAVAAKLAGKSFLAIRQAGDTGQLYGSVTTRDIAEVV
                     TAGGFTIDRRQIVLDRPIKTLGLQPTRIALHPEVIVQITLNVARSEDEAERQARGEDV
                     TVIKEEGVQIETFNAEALFEEEALARQRGEEPKAPDAVE"
     gene            3762..5012
                     /locus_tag="VFR00_10145"
     CDS             3762..5012
                     /locus_tag="VFR00_10145"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012496531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HEU0159666.1"
                     /translation="MLILLDVMLRRIVRRGDLTFIDAKGSSHRYGDGTGPALVVRVSQ
                     RRVEWHLVLDPQLALGESYMQGTLRMERGRIYDLLELLLSNAQRDPVPAWARALDVVR
                     YLIRRAAQFNPAGRAQRNVAHHYDIDGAIYDLFLDSDRQYSCAYFTPGADLEEAQLAK
                     KRHLAAKLAIDPGQRVLDIGSGWGGLAIYLAKAVGCEVTGITLSTQQHKISNERAARE
                     GLSRSAHFEFLDYRKVIGRFDRIVSVGMFEHVGVSHYGTYFKKVHELLTDKGVALIHT
                     IGRSEPPTVTNPFIAKYIFPGGYIPALSEVVAAIERSQLIITDVEILRLHYAQTLRAW
                     RERFLANWDKAAAILDERFCRMWEFYLAGSETAFRYQGLVVFQLQLAKRVGTLPITRD
                     YIWDDEQRLLAQDAIDRRPRLAGE"
     gene            5142..6671
                     /locus_tag="VFR00_10150"
     CDS             5142..6671
                     /locus_tag="VFR00_10150"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013418624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEU0159667.1"
                     /translation="MSQSPQRSLPAPPLSASDGPATAFRSAPHNLEAEQALLGAILIN
                     NEAHDRVSHFLEPHHFYDPLHQQIYETAAKLIGSGKQATPITMRTFFEHAERIDATLT
                     VPQYLGRLAANAATIINARDYAQTIYDLAIRRHLILIGEDMVNAAYDAPVDFPPREQI
                     EEAETRLFSLAETGKYGQGFRAFNNALVSAIEMANNAFQRDGGLSGIATHLRDLDGKL
                     GGLQSSDLIILAGRPSMGKTALATNIAFNVARSRARSLAERAELAADDQSHDGAVVGF
                     FSLEMSAEQLATRILSEQAGIPSEKIRRGMIDEPEFKRLVEASREMSSIPLFIDETGG
                     ISIAQLAARARKLKRQQGLGMIVVDYLQLLTGSQRRASEGRVQEVSEITTGLKALAKE
                     LNVPILALSQLSRAVENREDKRPQLADLRESGSIEQDADVVMFVFREEYYVERTKPSE
                     GTLEFQDWMAKMQIVSGKAEVIIGKQRHGPVGTVQLQFESNVTRFSDLARDAYLPERI
                     G"
     gene            6668..7834
                     /gene="alr"
                     /locus_tag="VFR00_10155"
     CDS             6668..7834
                     /gene="alr"
                     /locus_tag="VFR00_10155"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEU0159668.1"
                     /translation="MSRREGVSRGNRWPRAPVPPTARAALSVDLVALRANWARLNAAS
                     GRAECAGVIKADAYGLGLAPIAKALTSEGCKTFFVATIEEGRAARAVQPGATIYVLDG
                     LLPGAEPHYAGFDLRPVLSSLAEVRDWAAYCRARGRKLSAAVHLDTGLNRLGMPEGEI
                     ERLAAEPGLLAGFDMCLVMSHLACADEPANPMNERQRQRFDWLRAKLPAAPASLANSG
                     GTFLGAPFHYDLVRPGIALYGGRAHEGKPNPMQTVVRFAAKILQIRDLEPGATIGYGA
                     IYRVQRPSRIATIGCGYADGFLRALSVKTGEAGPVGFLGDFAVPIVGRVSMDFTTVDV
                     TDVPLELARRGAWVEVMGARVTVDDLTDRAGTIGYELLTRLGPRVHRVYEGAGS"
     gene            7839..9218
                     /gene="radA"
                     /locus_tag="VFR00_10160"
     CDS             7839..9218
                     /gene="radA"
                     /locus_tag="VFR00_10160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEU0159669.1"
                     /translation="MPRPARNAYVCQNCGVSAARWTGRCTACNEWNSLVEEASAAPPG
                     SGLATASKGRVLALESLSVANTPAMRRFSSGSSELDRVTGGGIVPGSALLIGGEPGIG
                     KSTLLLEVAARLAAAGRRALYFSGEEAAAQVRLRAERLGVASAPVALACDSNLANILA
                     TLNDGPAPDLVIVDSIQTLWVDGLEAAPGTVAQVRAAAHALVRFAKASGSALVLVGHV
                     TKDGQIAGPKLVEHMVDTVLYFEGDRGHPYRILRATKNRFGATDEIGVFEMAGEGLRE
                     VANASELFLPDRQASAPGSAVFAGIEGTRPILVEMQALVVPSGLGTPRRAVVGWDSNR
                     LAMLLAVLDARAGVSFAAQDVYLNVAGGLKISEPAADVAAAAALLSSSRNVALAADRV
                     FMGEIALSGAVRAVRHTPLRLKEASKLGFKTAVLPATAEAGAERDPLQRRGLAHVRDL
                     LDVLPPPAR"
     gene            9525..10178
                     /locus_tag="VFR00_10165"
     CDS             9525..10178
                     /locus_tag="VFR00_10165"
                     /inference="COORDINATES: protein motif:HMM:NF014704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEU0159670.1"
                     /translation="MSGVGPFTNLDLAVIAIVVLSGLVAMYRGLTREVLSILSWAAAA
                     GAVFYFIRYHRDIAEEVAKQFANPVQTSYVYVAQVAIGGLIFLVVLIIVHLITARISD
                     SVLDSRVGAIDRILGLAFGMVRGFVLIVIPYMFAVSFVCKDNPARSSAQGCRPGELPG
                     WVENAQSLPLINQTSGTLFSLLSRYAPSVPLGTGEQRQGWLPLDHISPNALTSSNSG"
     gene            10274..11764
                     /gene="purF"
                     /locus_tag="VFR00_10170"
     CDS             10274..11764
                     /gene="purF"
                     /locus_tag="VFR00_10170"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEU0159671.1"
                     /translation="MQGSLSTAETAAGVALPRGDDDRLREECGVFGVFGNEDAAALTA
                     LGLHALQHRGQEAAGIVTYDGSHFHSERRLGLVGDNFNKADVIARLKGRIALGHNRYS
                     TTGDTILRNVQPLFADLDTGGFAVAHNGNLTNALTLRRELISSGAICQSTSDTEIILH
                     LMARSQRRRVIERFVEALRQLEGAYALVCLTNDMLIGARDPIGIRPLVLGRLGGGYVL
                     ASETCALDIVGAEFVREIENGEIVTITVDGVESHRPFPKRPARPCIFEYIYFARPDSI
                     VGGRNVYELRKQMGVQLAREAPAQADVIIPIPDSGVPAAIGYAQESGIPFELGIIRNH
                     YVGRTFIEPEQRIRQLGVKLKHSANASAVRGNRVVLIDDSVVRGTTSKKLVQMMFEAG
                     AREVHLRVSSPPITHPDFYGIDTPRTTELLAANMSVEEMRAFTGCTSLAFLSVGGIYR
                     ALGIERRDFRAPQFTDHCFTGDYPTELTDQNGEAHASQLSFLAEVR"
BASE COUNT         1942 a         4071 c         3960 g         2075 t
ORIGIN      
        1 ggcatcgctg atcggtgccg ccaggacgtt ggcaacgacc ggcacctgcg gcgaggagat
       61 cttaacctgc gccagcgcct gcgccatggc ctcggcggcc ggccgcatca aggccgagtg
      121 gaagggcgca ctgacgttga ggggcatgaa gcggcgcaca ccgtgcgcct tggcgagctt
      181 gccggcgcga tcgatggccg ccttggagcc cgagatcacg gcctgggtcg gctcgttgtc
      241 gttggcaagc tggcagattt cgctctccgc cgcctcggcg gcgagcttct ccgccacctc
      301 cttgccgacc cccaacaggg ccgccatcgc cccttgcccg acggggacgg cggcctgcat
      361 ggcctgcccg cgcagtttca aaagccgggc ggcgtcggcg aggctcaaag ccccggccgc
      421 ggccagggcg gaatattcgc cgagcgagtg gccggcgacg caactcaccc ggtcggcgag
      481 ccggaaacct ttctccacct ccagcacgcg catgaccgca agcgagaccg ccatcagggc
      541 gggctgggca ttctccgtca gcgtcaactg ctcgagaggg ccggcaaaca tcagttcgga
      601 gagcttttgg ccgagcgccg cgtcgatctc ggcaaacacc gcccgcgccg gcgcgtaggt
      661 ggccgccagc tcggccccca tgcccaccac ttgactgcct tgtccaggga aaacgaaagc
      721 gatcgccatg gccgttcaag gattactgcc tcgcgcgggc ttcaccacac tcccaccccc
      781 acgtcaagat tgcccacgcc tgtgcccttg tcggcgccgc aaattcagct ataacgacgc
      841 ccgcacccgc tccggtcgaa ggctgaaggg gaggccgtcg cgagacggca gggcctaggt
      901 gggcccgcct accccggtgt cttcgctctt tcgggtcctt tgggccttcc cgcgccgacc
      961 ggcgtgccgg cccaaaaggg gctttgcgcc ggaggggata gggttaaaga gcaagggaag
     1021 gtggccatgc cgctttatga gcatgtattc ctggcgcgcc aggatatttc gcagacgcag
     1081 gtcgaagcgc tgacgaaaga atatagccaa gtcatcgagg aaggcggggg caaggtcggc
     1141 aagaccgagt actggggcct caagaccatc gccttcaaaa tcaaaaagaa ccgcaaggcg
     1201 cactactcgc tgatgaacat caatgcgccg ccggccgcgg tcgccgaaat ggaacggcgc
     1261 atgggcttgt cgagcgacgt cattcgcttc atgacggtgc gtgtcgacgc gcacgagacc
     1321 gagccgtccg tgcagatgcg caagggcgat cgcgatgacc gccgcgacgg cgatcgcggt
     1381 ggctttcgcg gcgatcgcgg tggcttccgc ggcggcgatc gcggcggctt ccggggtggc
     1441 ttccgtgggg gcggcggtgg cggcttccgg cgcgagggtg gtgagggcgg cttccggcgc
     1501 gaggggggcg gcgagggcgg cggcttccgg cgtgaggggg gtgatggcga caggccgcgc
     1561 ggcccccgtc caccgcgcga tgaggccgaa agcacgagcg ccgaggagta agaccgatga
     1621 gcgaagtcac caccattccc gcccggcgcc cgtttcaccg gcgccgcaag acctgcccat
     1681 tctcgggtga taacgccccc aagatcgact acaaggacat gcgtctgctg ggccgctacg
     1741 tctctgagcg cggcaagatc gtgccgagcc gcatcacggc cgtgtcggcc aagaagcagc
     1801 gcgaactggc gcgggccatc aaacgggccc gcttcatcgg gctgttacct tatctgatca
     1861 agtaagcggg tgccgggatc gccccggcct ctttatcgca aggccaatgg ttgggacggc
     1921 ggcgccgtct ctaaccgcta cggcgggaca gctgttcaga tgccgatcgg tttgctaaca
     1981 ggcgttggct cgggacttgc ctccgcgttg ctgttctact cggcgacgcg gggctcaccg
     2041 ctcttgagct cgttcctgct gctcttgacg cccttgcccg cgttgatcgc cgggctgggc
     2101 tggggttggc tgccggcgct gctcggcggt gtggtcgcct ccgcggcaat gctcgccatc
     2161 gccagcctgc ccttcgcggt gggttacttt ttggcccttg gcggaccctc cgcgctctcc
     2221 gcctaccttg cctatctcaa tcgcaccgac gccgagggcg tggtgcagtg gtatccgccc
     2281 ggtcgcctgc tcgccgcctt ggcactttac ggcggcgcgc tccccatcct ggtgctgccg
     2341 atgctgggcg gcagctacga gatcctgcgc gagcccatgg ccgagtactt ccggcgcttt
     2401 tccagccgca cggcgagcga cctcaatatg ccgccgctca acgaggccca gatcgaaagt
     2461 ctgacggaat ttctgatcgc cgtgctgccg gcggccctgt cggcctactg gctgatcctc
     2521 tttaccatca atctctatct tgccgcccgc gtcgcccgcg cctcggaccg cttggggcgg
     2581 gactggcccg atctggcagc gctcgcctac cccccaggct tccccctgat cgccgttgcc
     2641 gcctttgcgg cgtcttttgc gccgggcgtg ctcggtctgc tggggaccag tttcagcggc
     2701 gcgctgctcg tcgcctatct gatcgccggt cttgccctca tgcattttct ggcccggggg
     2761 cgggcgcctt ggctgctatg gctcgtctac ggggggcttt tcctgctcgg tccctatgcg
     2821 gccctggggc tgaccgtcgc cggcctgctc gagcccgtca tcaatctgaa gcgccgcctc
     2881 ggcgccccac cctcgtcgac gtgaaccgca cgggaagcac gctcgcacca ccagccttgg
     2941 acaacactgt tagacaccac ttggagagaa cgtatgcaga tcattcttct tgagcgcatc
     3001 ggccggctcg gccagatggg cgatgtcgtc aatgtcaagg acggctatgc ccgcaacttt
     3061 ctgttgccgc aaaaaaaggc attgcgcgcg accgcggaaa acctcgcccg cttcgaaaag
     3121 gagcgctccc aactcgaagc gcgcgacctg gaactgaaaa aggaggccga ggcggtggcc
     3181 gccaagctcg ccggcaagag cttcctcgcc atccgccaag ccggggatac aggccagctc
     3241 tacggttcgg tgaccacccg cgatatcgcc gaggtggtga ccgccggcgg ctttacgatc
     3301 gaccggcgcc agatcgttct tgatcgtccg atcaagacgc ttggcctgca acccacccgc
     3361 atcgccctgc accccgaggt gatcgttcag atcacgctca atgtcgcccg ctcggaggat
     3421 gaggcggagc ggcaggcgcg cggcgaggac gtgaccgtga tcaaggaaga gggcgtccag
     3481 atcgagacct ttaacgctga ggcgctgttc gaggaggagg cgctggcgcg tcagcgcggc
     3541 gaggaaccca aagccccgga cgcggttgag tagccgcgcc tcctctgttg ctgtccgcgg
     3601 ggcacggacg gcaggacgcg actacgagtt gccgtgcaaa ccgaaaccgc aagggcaagc
     3661 ttagcggtga cattgtgcac gactttcgta taatgcggga aaacaaggcc tgaggccgaa
     3721 gggaggaacg gtcgaggact gcatacgcgg cagcgtgcgt catgttgatt ttgctcgatg
     3781 tcatgttgcg ccgcatcgtc cggcgcggcg atctgacctt catcgatgct aaggggtcct
     3841 cgcatcgcta tggcgacgga accgggccgg cactggtcgt gcgcgtcagc cagcgccgcg
     3901 tcgagtggca cttggtgctc gatccgcagc tggccctggg cgagagctac atgcagggca
     3961 cgctcaggat ggagcgtggg cgcatctatg acttgctcga gctcctgctg agcaacgccc
     4021 agcgcgaccc ggttccggct tgggcgcggg cgctcgacgt tgtgcgctac ctgattaggc
     4081 gtgccgcgca gttcaatccc gccggccggg cccagcgcaa cgtggcgcac cactacgaca
     4141 tcgacggggc aatttacgat ctcttcctcg acagtgaccg gcagtactcg tgtgcctatt
     4201 tcacgcccgg cgcggatttg gaggaagccc agctggccaa gaaacggcac ctggcggcca
     4261 agctcgccat cgaccccggc cagcgcgtgc tggacatcgg ctcgggctgg ggtgggctcg
     4321 ccatttatct ggccaaggcg gtgggctgcg aggtgacggg tatcacgctc tccacccaac
     4381 agcacaagat ctccaacgaa cgtgcggccc gggaaggctt gagccgatcg gcccatttcg
     4441 agttcctgga ctatcgcaag gtgatcggcc gcttcgatcg catcgtctcg gttggcatgt
     4501 tcgagcacgt cggcgtctcc cactacggca cctacttcaa gaaggtgcac gagctcctca
     4561 ccgacaaggg cgtggccctg atccacacca tcggacgctc cgagccgccg accgtgacca
     4621 atcctttcat cgccaaatac atcttccccg gcggctatat tcccgccctt tcggaggtgg
     4681 tggcggcgat cgaacgttcg caactcatca tcaccgacgt cgagatcttg cgcctgcact
     4741 atgcgcagac cttgcgcgcc tggcgcgagc gcttcctggc caactgggac aaggcggccg
     4801 cgatcctaga cgagcgcttc tgccggatgt gggagttcta tctcgccggc tcggagacgg
     4861 ccttccgcta ccagggtctg gtggtgtttc agctacagct cgccaagcgc gtcggcacgc
     4921 taccgatcac gcgcgactac atctgggacg acgagcagcg cctgttggcg caagatgcga
     4981 tcgatcgccg gccgcggctg gccggcgagt agcgccgccg cagggcagcg attcggccgg
     5041 ccaagggctt tctgggcgcc gatgtgcaaa acagggatgg tcgggcttga cagccaagtc
     5101 ctcggccacg cccgcggcgg aatcggctat tgcctcagcg catgagccaa tccccccagc
     5161 gatctttgcc cgcaccaccc ctgtcggcgt ctgacgggcc ggcaaccgcc ttccgcagcg
     5221 cgccgcacaa cctcgaagcc gagcaggccc tgctcggcgc catcctcatc aacaacgagg
     5281 cgcacgaccg cgtttcgcac tttctcgagc cgcaccactt ctacgaccca ctgcatcagc
     5341 agatctacga gaccgcggcc aagttgatcg gctcgggcaa gcaggcgacg ccgatcacga
     5401 tgaggacgtt tttcgagcac gccgagcgca tcgatgcgac cttgacggtg ccgcagtatc
     5461 tcggccgcct ggcggccaac gccgccacca tcatcaacgc gcgcgactac gcgcagacca
     5521 tttacgacct cgccattcgc cgacacctca tcctgatcgg cgaggacatg gtcaacgccg
     5581 cttatgacgc tcccgtcgat tttccgccgc gtgagcagat cgaggaggcc gagacgcggc
     5641 tcttctcgct cgccgagacg ggcaaatacg gtcaggggtt ccgcgccttc aacaatgccc
     5701 tcgtcagcgc catcgagatg gcgaacaacg ccttccagcg cgacggggga ttgtcgggaa
     5761 ttgccacgca cctgcgcgat ctcgacggca agctcggcgg tctacagtcc tcggacctga
     5821 tcatcctggc cggccggccc tccatgggca agacggcgct cgccaccaac atcgccttca
     5881 atgtggcgcg cagccgcgcg cgctcgctcg ccgagcgggc ggagcttgcc gccgatgatc
     5941 agagccacga cggcgcggtg gtgggctttt tctcgctcga gatgtccgcc gagcagctcg
     6001 cgacccggat cttatcggaa caggccggca taccttcgga aaagatccgc cgcggcatga
     6061 tcgatgagcc ggaattcaag cgcctggtgg aggcgagccg ggaaatgtcg tccattcccc
     6121 tcttcatcga cgaaacgggg ggcatctcca ttgcccagct ggcggcgcgc gcgcgcaagc
     6181 tcaagcgtca acagggcctc ggcatgatcg tggtggacta cctgcagctg ctcacgggct
     6241 cgcagcggcg ggcctcggag ggacgcgtgc aggaggtctc ggaaatcacc acggggctga
     6301 aagccctggc gaaagagctc aacgtgccga tcctggcgct ctcgcagctc tcccgcgccg
     6361 tggaaaatcg cgaagacaaa cggccgcagc tcgccgacct gcgcgagtcc ggctcgatcg
     6421 agcaggacgc cgacgtcgtc atgttcgtct ttcgcgaaga gtattatgtt gagcgcacca
     6481 agccctccga agggacgctc gagtttcagg attggatggc taagatgcag atagtgagcg
     6541 gtaaagccga ggtgatcatc ggcaagcagc ggcacggtcc cgtgggaacg gtgcagctcc
     6601 agtttgagtc caacgtcaca cgcttttccg acttggcgcg cgacgcttat ctgccggagc
     6661 ggatcgggtg agccgccgcg aaggcgtctc ccgcggcaac cgttggccgc gtgcgccggt
     6721 gccgcccacc gcccgggcgg cgctatccgt cgatctggtg gcgctgcgcg ccaattgggc
     6781 acgcctcaat gccgcctccg gccgggccga gtgcgccggc gtgatcaagg ccgacgccta
     6841 cggcctgggc ctggcaccga tcgccaaggc gctgaccagc gagggctgca aaaccttctt
     6901 tgtggcaacc atcgaggagg gccgcgcggc gcgggcggta cagccgggcg ccaccatcta
     6961 tgtgctcgac ggcctcctgc cgggggccga gccgcattac gccggcttcg acctgcgccc
     7021 ggttctttcc agcctcgccg aggtgcgcga ctgggcggcc tattgtcggg cccgcggccg
     7081 caagctctcg gcggccgtgc accttgacac ggggctcaac cgcctcggca tgccggaagg
     7141 cgaaatcgag cggctcgccg ccgagccggg cctgctcgcg ggcttcgata tgtgcctggt
     7201 gatgagccat ctcgcctgcg ccgacgagcc ggccaacccc atgaacgagc gccagcgcca
     7261 gcgcttcgat tggctgcgcg ccaaacttcc cgccgcgccg gcgagcctcg ccaattccgg
     7321 cggcaccttc ctgggcgcgc cctttcacta cgatctggtg cggcccggca tcgcgcttta
     7381 cggcgggcgt gcgcacgagg gcaagccgaa cccaatgcaa accgtcgtcc gcttcgccgc
     7441 gaaaatcttg caaatccgcg atctcgagcc cggggcgacc attggctatg gcgccatcta
     7501 ccgggtacag cgaccctcgc gcattgccac catcggttgc ggctatgccg acggcttcct
     7561 gcgcgcgctc agcgtcaaga ccggcgaggc gggtccggtg ggcttcctcg gcgacttcgc
     7621 cgtacccatc gtcggccgcg tctccatgga ttttacgacc gtcgacgtca ccgatgttcc
     7681 cttggagctg gcgcgccgcg gtgcctgggt ggaggtgatg ggcgcccgcg tcaccgtcga
     7741 cgatctgacc gatcgggcag gcacgatcgg ctacgaactt ctgacccggc tggggccgcg
     7801 cgtgcaccgg gtttacgaag gagcggggag ctgaggccat gccacgcccg gcccgaaacg
     7861 cctacgtctg tcaaaactgc ggcgtcagcg ccgcgcgctg gaccggcagg tgcacggcct
     7921 gcaacgagtg gaacagcctg gtcgaggagg ccagcgccgc cccgcccggc agcgggctgg
     7981 cgacggccag caagggtcgc gtgctggccc tggaaagcct cagtgtcgcc aacaccccgg
     8041 cgatgcggcg cttttcgagc ggcagcagcg agctcgaccg cgtcacgggt ggcggcatcg
     8101 tgcccggctc ggccctgctg atcggcggcg agccgggcat cggcaagtcg accctgttgc
     8161 tcgaggtggc ggccaggctg gcggcagctg gccggcgtgc gctttatttc tccggggagg
     8221 aggccgccgc ccaggtgcgg ctcagggccg agcgcctcgg ggtggcgagc gcgccggtgg
     8281 cgctcgcctg cgacagcaat ctcgccaaca tattggcgac gctcaacgac ggcccagcgc
     8341 ccgacctcgt catcgtcgat tcgatccaaa ccctgtgggt cgatgggctg gaggcggcgc
     8401 cggggacggt ggcccaagtg cgggctgcgg cgcatgccct ggtgcgcttt gccaaggcga
     8461 gcggcagcgc cttggtcctg gtcggccacg tcaccaagga tggccagatc gccgggccca
     8521 agctggtgga gcatatggtc gacaccgtcc tctacttcga aggcgaccgc ggccatccct
     8581 atcgcatcct gcgcgcgacc aagaaccggt tcggggccac cgatgaaatc ggggtgttcg
     8641 agatggcggg cgagggcctg cgcgaggtcg ccaatgcctc cgagctgttc ctgccggatc
     8701 gccaggcctc cgcgccaggg tcagcggtct tcgccggcat cgaaggcact cgcccgattc
     8761 tcgtggaaat gcaggccctg gtggttccct cggggctggg caccccgcgg cgcgccgtcg
     8821 ttggctggga tagcaatcgg ctggccatgc tgctggcggt cctggatgcg cgagcgggcg
     8881 tgagcttcgc tgcccaagac gtctatctca atgtggcggg aggcttgaag atcagcgagc
     8941 cggcagccga cgtggcggct gcggccgctc tgctgtcctc gagccgcaac gtggcgctcg
     9001 ccgccgatcg cgtgttcatg ggcgagatcg cgctctccgg ggccgtccgc gccgtccggc
     9061 acacgccact gcgcctgaaa gaggcaagca agctcggctt caagaccgcc gtgctgccgg
     9121 ccaccgccga ggcgggcgcc gagcgcgacc ccctgcagcg ccggggcttg gctcacgttc
     9181 gcgaccttct cgacgtgctg ccgccgcccg cgcgttaagg caacgggagg ccggcggcgg
     9241 gcgggcgcca cgagcgaccc acccacaata cccccacaat tgtgcccacc cagcatcacc
     9301 gcatctggcc gagtacagct atattgtgtt cgggcaggag ctgccctaga gcctagcccc
     9361 gggcaacgtg gcgaacctgg ctcgagcacg ggggagcgga ttttgacctg ggcggatttg
     9421 agcggagcga agcctcgccg gggcgccggt cgggctccgg ggcttagccc gctgtgcgcg
     9481 ctgcccgcac ggccatcctg tcgctggatc gaggaccctc gaaaatgagc ggagtgggac
     9541 cctttaccaa tctcgacctg gcggtgatcg ccatcgtcgt gctctcgggg ctggtggcga
     9601 tgtatcgcgg cctgacgcgg gaggtgctct cgatcctgtc ctgggcggcg gcggccggtg
     9661 cggtcttcta ttttattcgc tatcaccgcg acatcgccga ggaggtcgcc aagcaattcg
     9721 ccaacccggt gcaaacctcc tacgtctatg tggcccaggt ggcgatcggc ggtttgattt
     9781 tcctggtcgt cctgatcatc gttcatctta tcacggcgcg gatttccgat tcggtcctcg
     9841 acagccgggt gggcgcgatc gatcgcatct tgggcttggc cttcggcatg gtgcgcggct
     9901 tcgtgctgat cgtcattccc tacatgtttg ccgtctcctt tgtgtgcaag gacaatccgg
     9961 cacgctcgtc cgcgcaaggc tgtcggcccg gcgagctgcc gggctgggtg gagaatgccc
    10021 agtcgttgcc gctgatcaac cagacgagcg gcacgctgtt ttccctgttg tcgcgctatg
    10081 cgccttcggt gcccctaggc acgggcgagc agcgtcaggg ctggctgccc ctcgatcata
    10141 tcagccccaa cgccctcacc agtagcaact cgggctgact tgccctatat ccagctcgcc
    10201 tgaggtcgtc gccgcagatt cgggatgagt gggcttggcg gtacgccgga ctttcccctt
    10261 aacgaggctt cccatgcaag gttcgctctc aacggctgag accgcggcgg gcgtcgccct
    10321 gccgcgtggc gatgatgatc gtctgcgcga ggaatgtggc gtcttcggcg tgttcggcaa
    10381 tgaagacgca gcggcactca ccgcgcttgg gctgcacgcc ttgcagcatc gtggccagga
    10441 ggctgcgggg atcgtcacct atgatggcag ccactttcac tccgagcgcc ggttaggcct
    10501 ggtcggcgac aatttcaaca aggccgacgt gatcgcacgc ctcaagggtc gcatcgccct
    10561 tggccataac cgctattcca ccaccggcga caccattttg cgcaacgtgc agcccttgtt
    10621 cgccgacctc gacacgggtg gttttgccgt ggcgcacaac ggcaatctta ccaacgcgct
    10681 gacgttgcgg cgcgagctga tctcgagcgg cgccatttgc caatccacct ccgataccga
    10741 gatcatcctg cacctgatgg cacgctcgca gcgccgccgc gtgatcgagc gcttcgtgga
    10801 agcgctgcgc cagctcgaag gcgcctacgc cctcgtctgt ctcaccaatg acatgctgat
    10861 cggggcgcgc gatccaatcg gcatccgtcc gctcgtgctc ggccggctcg gtggcggcta
    10921 tgtgctggcg tcggagacct gtgcgctcga catcgttggc gccgaattcg tgcgcgagat
    10981 cgagaacggc gaaatcgtca ccatcaccgt cgatggtgtc gagagccatc gacccttccc
    11041 caaacgcccg gcgcgcccgt gcatcttcga gtacatctac ttcgcccgcc ccgattccat
    11101 cgttggcggg cgcaacgttt atgagctgcg caagcaaatg ggcgtgcagc tcgcccgcga
    11161 ggcccccgcc caggccgatg tcatcatccc gatccccgac tccggggtgc ccgcggcgat
    11221 cggctacgcc caggagagcg gcatcccctt cgagctcggc atcatccgca accactacgt
    11281 cgggcgcact ttcatcgagc ccgagcagcg catccgccag ctcggcgtga aactcaagca
    11341 ttccgccaat gccagcgcgg tgcgcggcaa ccgcgtcgtg ctgatcgatg acagtgtcgt
    11401 gcgcggcacg acctccaaga aactggtgca gatgatgttt gaggcgggcg cccgcgaggt
    11461 gcatctgcgc gtctccagcc cgcccatcac gcatcccgac ttctacggta tcgacacgcc
    11521 gcgcaccacc gagctgttgg ccgccaacat gagcgtcgag gagatgcgtg ccttcacggg
    11581 ttgtaccagc ttggcgttcc tgtccgtggg cggcatctac cgggcgctgg gcatcgaacg
    11641 gcgcgatttt cgcgcgccgc agttcaccga tcattgtttt accggcgact atccaaccga
    11701 gctcaccgat cagaacggcg aggcgcacgc gagccagctg tcattcctgg ccgaggtgcg
    11761 ttgaggcccc gagcgagccc cctgctttcg tagcttggcg ttcgtgcggc aaccgcccag
    11821 gcggcggctg gcggcgcccc gatgccggcg cgttggcggc gcgtgccggg caaagcttgg
    11881 gacgcgcggg gtcggccggg ccttgccagg ggcccgagat ccccgctatc acgccaagca
    11941 gcgaaaaagc tgagggacgt ccctgatgag tttagcagcg cgcgccgagg atgacgcggg
    12001 gctggcgggc aaggtcgcca tcgttgccgg tggcggcgcc gcggagga
//
LOCUS       DASTMT010000437         5662 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_3527789, whole genome shotgun sequence.
ACCESSION   DASTMT010000437 DASTMT010000000
VERSION     DASTMT010000437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 5662)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5662)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5662
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3527789"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..971)
                     /locus_tag="VFR00_10175"
     CDS             complement(<1..971)
                     /locus_tag="VFR00_10175"
                     /inference="COORDINATES: protein motif:HMM:NF016992.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HEU0159672.1"
                     /translation="MATIAQAIETTGGGVIQFLKWWRDELAGLLPQRAQGRSAARILV
                     AITPEEGYRVFEGTGAKLRPVRAGLELSALDAVGAAADLAKSNPGALLGIRVPLASCF
                     VRSLELPRSAQDDASRILDLDLERATPFKRKDVYCATLLESKDDRSPQVRVRHLMIKR
                     QSVEPVLSELNASGASVDFIDCWDESTGQALPVDFLASQQQEATAARRGLSFATLLLA
                     LLGLLGASALVLANARYQTALQTVEAQVAQARAQATNVRRALDTSEAALTEIDQLQRL
                     KLTTTPTVELIDALTKLMPNTVWLTDLRLENGALDITGLAKSGAALLP"
     gene            1104..2846
                     /locus_tag="VFR00_10180"
     CDS             1104..2846
                     /locus_tag="VFR00_10180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016918352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HEU0159673.1"
                     /translation="MTQQSAAAHGGASDPRSEEFVAALAAFLLERNALDELAVHRAQR
                     AQKQSGERFDLVLTRLGLLPDVELARLLAEFLGMPLAEAKDLPQVPLLAEHLELNFLA
                     SNRLLPLAEDGESIVVATADPFNTDPIAAIGFLLGRPVEARLMAASVVERAIQEIYGR
                     AGALGPEQEATGQASDEDVRRLEDLASEAPIIRLAHDLITRAVDMQASDIHVEPREDS
                     LRVRYRLDGMLHTVETLPLSVRAAVTSRIKIMAQLNIAERRLPQDGRIKTTVHGREID
                     LRVSTMPTLCGESVVLRILDRSSVQLDFQSLGFAGPAHEAFAKLLNQPNGIILVTGPT
                     GSGKTTTLYTALNALNTSESKTFTVEDPIEYQLVGVNQIQVQPKIGLSFAHALRSILR
                     QDPDIIMVGEIRDLETAQMSIQASLTGHLVLSTVHTNSASATVTRLLDMGVADYLLSS
                     TLTGVLAQRLVRRLCQACAAPVPDSELLLERLRHQPDGVTRALPKDGRGLRNKTGCKA
                     CRNTGYLGRTTISELLVVTDPVRRQILESGTEAAIQQAALEAGMVSMFDDGLAKALRG
                     ETTIDEVLRVTRMN"
     gene            2846..4075
                     /locus_tag="VFR00_10185"
     CDS             2846..4075
                     /locus_tag="VFR00_10185"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEU0159674.1"
                     /translation="MPRYSYKAYDAKGGRLDGEIESETREAALEALVRQGRYALELVE
                     GGGKAPSLPWWQRDLFAARRLSQQNIALLTRELATLVKAELPIDQALRIVVLQPLISA
                     RTRQVVAAVLARVLDGAALSEALSAQQGAFPEFYWRIVHAGEASGTLGPALDDLAHLL
                     DRSMEFRARIVSALVYPATLLLAAGAALVIVLTILIPTIAPMFKEAGKEPPALIGSLI
                     AFQDFLSEHWFVTLVAGGLALAALIGLFRQEAWRLMRDRMMLRLPLISGLVENGQTAV
                     FARTLGTMIKSGVPMLRALGVAADALRNRAMSGAVHACASEMREGGGLVGSLGKSGLF
                     PELSLRLIGVGEQSGQLDTMLGRVADIYEAALQRQLDRVTSLLTPILTVVIGVVVGGL
                     ILSVIGAIVSVNELALQ"
     gene            4072..4536
                     /locus_tag="VFR00_10190"
     CDS             4072..4536
                     /locus_tag="VFR00_10190"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family pseudopilin"
                     /protein_id="HEU0159675.1"
                     /translation="MTRPLGEGQPRYEEGFTLIELIVALGIMALGVAVAFPYFEGTRA
                     GHQLRATAYEIAGQLRDARATAQARNIDQAVILDVGNRRMWLDGSEVRRALSPRMMVD
                     VEVPAAEQLASSVVRVRFFADGSASGGKVVLRDGGRRAIVSVNWLTGDVRVE"
     gene            4538..4954
                     /locus_tag="VFR00_10195"
     CDS             4538..4954
                     /locus_tag="VFR00_10195"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEU0159676.1"
                     /translation="MARRCCSGERDAGFTLMETIVALIIFVACYVLIQQGLSLGWRGV
                     SASQGELAALHVAQAHLAATGVEAPLSEGQQAGNDNGYAWVVDVRRQNAEAESESRAK
                     THPTGYWVAVEVSWRDEVTRRQRSLQLKTLKVALPQ"
     gene            4951..5604
                     /locus_tag="VFR00_10200"
     CDS             4951..5604
                     /locus_tag="VFR00_10200"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEU0159677.1"
                     /translation="MKAVAANPVRAAEAGFSLMEMLVSLVILALILAFLPGAFRLARQ
                     VWTATAVLDRDGGRDLGLDFLETRLAEAMPLYEPAKSGLVNIVFRGGKDSLRFVAPSR
                     NGPAGGGLYRYSIEQRPNAGGTTSSLVVQVAPYLGPVREGTSPPDSEEQVLADDVVSA
                     EFRYFGRHELRQAASWQAQWKRTDALPDLVELTIQRHRAGGMSNRVITTELRLRNSS"
BASE COUNT          892 a         1896 c         1910 g          964 t
ORIGIN      
        1 ggcagcaggg cggcgccgga cttggcgagc ccggtgatgt cgagtgcccc gttctccagg
       61 cgcaaatcgg tcagccatac cgtgttgggc atgagcttgg tcagggcatc gatgagctcc
      121 accgtcgggg tggtggtgag cttcaggcgc tgcagctggt cgatctcggt cagcgccgcc
      181 tcggaggtat cgagcgcgcg gcggacgttg gtggcctgcg cacgggcctg cgcaacctga
      241 gcctccacgg tttgcagcgc tgtttgatat ctggcgttgg cgagcacgag ggcggacgca
      301 ccgagcaagc ccagcagggc cagcagcagg gtcgcgaagg aaagcccgcg ccgagcggcg
      361 gttgcctctt gctgctggga ggcgaggaag tcgacgggca gggcctggcc ggtgctctcg
      421 tcccagcagt cgatgaagtc gacgctggcg ccggaggcgt tgagctccga caggacgggc
      481 tcgaccgact ggcgcttgat catcaggtgg cgcacgcgca cctgggggct gcggtcgtcc
      541 ttgctttcca gcagcgtggc gcaatagacg tccttgcgct tgaacggggt ggccctttcc
      601 aggtcgagat ccaagatccg gctggcgtcg tcttgggcgc tgcgcggcag ctcgaggctg
      661 cgcacaaagc agctggcgag cggcacccgg atgccgagca gtgcgcccgg gttggacttg
      721 gcaaggtccg ccgcggcccc gacggcatcg agcgcagaaa gctccagacc ggcgcgcacc
      781 gggcgcagct tggcccccgt gccttcgaac acgcggtagc cctcctcggg ggtgatggcc
      841 acgaggatcc tggcggccga gcgtccctgc gcccgttgcg gcagcaggcc ggcaagctcg
      901 tcgcgccacc acttcagaaa ttggatgacg ccgccgcccg ttgtttctat ggcttgtgca
      961 atggttgcca tctacgctta accccctggc acgccgtttg cgcgcctgcg ccgagctcac
     1021 ggccggcgcg gcctctcgac cgcgttccaa tatactggaa ttcgtagtat ttttcccagg
     1081 tgtaattgta gcgcggggga gccatgaccc aacaatcggc cgcagcgcac gggggtgcct
     1141 ccgacccgcg cagcgaggag ttcgtcgccg ccttggcggc cttcctgctc gagcgcaacg
     1201 cgctcgatga attggccgtg caccgcgccc agcgggcgca aaaacagtcg ggcgaacgct
     1261 tcgatttggt gctaacccgg ctcggcttgc tgcccgacgt ggagctggcg cgcctgctgg
     1321 cggagttcct cggcatgccg ctcgccgaag ccaaggactt gccgcaggtt cccctgttgg
     1381 ccgaacacct cgagctcaac ttcctcgcca gcaaccgcct gctgccgctt gccgaggacg
     1441 gcgaaagcat tgtcgttgcc accgccgatc ccttcaacac cgatccgatc gcggcgatcg
     1501 gcttcctgct cggccggccc gtcgaagcgc gcctgatggc cgccagcgtc gtcgagcgcg
     1561 ccattcagga gatctacggg cgcgccggcg ccctgggccc ggaacaggag gcgaccggcc
     1621 aggcgagcga cgaggatgtg cgccggctgg aggatctcgc cagcgaagcg ccgattatcc
     1681 ggctcgccca cgacctcatc acgcgcgccg tcgatatgca ggcctccgat atccacgtgg
     1741 agccgcgcga ggactccttg cgcgtgcgct atcggctcga cggcatgctg cataccgtcg
     1801 aaaccctgcc cctgtcggtg cgcgcggcgg ttacctcgcg catcaagatc atggcgcagc
     1861 tgaatatcgc cgagcggcgc ctgccgcagg acggccgcat caagaccacg gtacacgggc
     1921 gcgagatcga cttgcgcgtt tcgaccatgc ccaccctatg cggggaaagc gtggtattgc
     1981 gcatcctcga ccgctccagc gtgcagcttg atttccagtc gctgggattt gccgggccgg
     2041 cgcacgaggc ctttgccaag ctgctcaacc agccgaacgg catcatcctg gttaccggcc
     2101 cgaccggcag tggcaagacg accacgctct atacggcctt gaatgcgctc aacacttccg
     2161 agagcaagac cttcacggtc gaggacccga tcgagtatca gctcgtgggc gtcaatcaga
     2221 tccaggtgca gcccaagatc ggcctcagct tcgcccacgc gctccgttcg atcctgcggc
     2281 aggaccccga catcatcatg gtcggcgaga tccgcgacct ggagacggca caaatgtcga
     2341 tccaggcctc gctgacgggg catctggtgc tctccaccgt gcacaccaac agtgcttcgg
     2401 cgaccgtcac gcgcctgctc gacatgggcg tggccgatta tctcctgtcc tcgacgctga
     2461 ccggagtgct ggcgcagcgc ctggtgcggc ggctatgcca ggcctgcgcg gcacccgtgc
     2521 cggattcgga gctgctgctc gagaggttgc gccaccagcc cgacggtgtg acgcgcgcgc
     2581 tgccaaaaga cgggcgcggc ctgcgcaaca agaccggctg caaggcgtgc cgcaacacgg
     2641 gctatctcgg gcgcaccacc atcagcgagc tcctggtcgt caccgatccg gtgcgccggc
     2701 agatcctgga aagcggcacc gaggcggcga tccagcaggc cgcgctcgaa gccggtatgg
     2761 tcagcatgtt cgacgacggc ttggccaaag ctctgcgcgg ggagaccacc atcgatgagg
     2821 ttctgcgcgt gacacggatg aactgatgcc gcgctattcc tacaaggcct atgacgccaa
     2881 gggcgggcgg ctcgacggcg aaatcgaatc ggaaacgcgc gaagccgcgc tcgaggccct
     2941 ggtgcgacag ggtcgctacg cgctcgagct cgtggaaggc ggtggcaaag cgccatcgct
     3001 accctggtgg cagcgggact tattcgccgc gcgccggttg tcgcagcaaa atatcgcgct
     3061 tttgacgcgc gagcttgcca cgctcgtcaa ggccgagctg ccgatcgacc aggcgctgcg
     3121 cattgtcgtg ctgcagccgc tgatctcggc gcgcacgcgc caggtggtcg ccgccgtgct
     3181 cgcgcgcgtg ctcgacggag ctgcgctctc ggaagccttg agcgcccagc agggtgcgtt
     3241 tcccgaattc tattggcgca tcgtgcacgc gggcgaagcc agcggtacgc tcggcccggc
     3301 gctggacgat ctggcgcatc tgctcgatcg ttccatggag tttcgcgccc gcatcgtctc
     3361 cgcgctggtc tatccggcca cgctgttgct ggccgccggc gccgccctcg tgatcgtcct
     3421 caccatcctc attcccacca ttgcccccat gttcaaggag gcgggcaagg agccgccggc
     3481 cctgattggc agcctgatcg ctttccagga tttcctctcc gagcactggt ttgtcacgct
     3541 cgtcgccggc ggtttggcac ttgccgcgct gatcgggctc ttccggcaag aagcctggcg
     3601 gctcatgcgc gaccgcatga tgctgcgcct gccgctgatt tccgggctgg tcgagaacgg
     3661 ccagaccgcc gtgttcgcgc gcacgcttgg aaccatgatc aagagcgggg tgccgatgct
     3721 gcgtgccctt ggcgttgccg ccgatgccct gcgcaatcgc gccatgtccg gcgcggtgca
     3781 cgcctgcgcc agcgaaatgc gtgaaggcgg ggggctggtc ggctcgctcg gcaaatccgg
     3841 gctttttccg gaactgtcgt tgcgtctgat cggcgttggc gagcagtcgg gacagctcga
     3901 taccatgctc ggccgcgtcg ccgatatcta cgaggcggcg ctgcaacgcc agctcgatcg
     3961 cgtcaccagc ttgctgacgc cgatcctcac ggtggtgatc ggcgtcgtcg tcggcggcct
     4021 gatcctgtcg gtgatcggtg ccatcgtcag cgtcaacgaa ttggccctgc aatgacgagg
     4081 cctcttgggg aaggtcagcc gcggtacgaa gagggtttca ccctgatcga gctgatcgtg
     4141 gcgctcggca tcatggcgct cggcgtggcc gtggcgtttc cctacttcga aggcacgcgc
     4201 gccgggcatc agctgcgcgc cacggcctac gagattgccg gccagctgcg cgatgcgcgg
     4261 gcgacggcgc aggcgcgcaa catcgatcag gccgtcatcc tcgatgtcgg caaccggcgc
     4321 atgtggctcg atggctccga ggtgcggcgc gcgctatcgc cgcgcatgat ggtcgacgtg
     4381 gaagtcccgg ccgccgagca gctcgccagc agcgtggtgc gcgtgcgctt tttcgccgat
     4441 ggcagcgcca gcggcggcaa ggtcgtgttg cgcgacgggg gccggcgcgc catcgtgagc
     4501 gtcaattggc tgaccggcga tgtgcgcgtt gagtaacatg gcccggcgct gctgtagcgg
     4561 cgagcgcgac gccggcttca ccttgatgga gaccatcgtc gccttgatca ttttcgtggc
     4621 ctgctacgtg ctgatccagc aagggctatc actgggctgg cgcggggtca gcgcctcgca
     4681 gggcgagctc gccgccctcc atgtcgcgca ggcccacctg gccgcaaccg gcgtcgaagc
     4741 cccgttgagt gaaggccagc aagccggcaa cgacaacggc tacgcctggg tggtcgatgt
     4801 gcgccgccag aacgcggaag ctgaaagcga gagccgcgcc aaaacgcacc cgaccggata
     4861 ttgggtggcg gtcgaggtga gctggcgcga cgaggtgaca cggcgccagc gctccctgca
     4921 gctcaagacc ctgaaggtgg cattgccaca atgaaagcgg tcgccgccaa ccccgtcaga
     4981 gccgccgagg cgggcttctc gctgatggag atgctcgttt cgctggtgat cctggccctg
     5041 atcctggcct tcctgcccgg cgcctttcgc ctggcacgcc aggtctggac cgcaaccgcc
     5101 gttctcgacc gggatggagg ccgcgacctg ggcctggatt ttctcgagac gcgcctggcc
     5161 gaagcaatgc cgctgtacga gccggccaaa tccggcctcg tcaatatcgt ttttcgcggt
     5221 ggcaaggaca gcctgcgctt cgtggcgccg tcgcgcaacg gcccggcagg cggcggcctt
     5281 taccgctata gcatcgaaca gcggcccaac gccggcggga caaccagctc gctggtggtg
     5341 caggtggcgc cctatttggg gcccgtgcgc gagggtacga gcccccccga cagcgaggag
     5401 caggtgctgg cggacgacgt cgtcagtgcc gagtttcgct atttcggacg ccatgagctg
     5461 cggcaagccg cgagctggca agcccagtgg aaacgcaccg atgcgcttcc cgatctcgtt
     5521 gagctcacga tccagcgcca tcgcgccggt ggcatgagca accgcgtcat caccaccgag
     5581 ctgcgcctgc gcaactcgag ttagccaaac ccggcgttag ggcctcggcg cgcgagccca
     5641 gcgcaggctc gtgcctgccg gc
//
LOCUS       DASTMT010000438         4932 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_2540185, whole genome shotgun sequence.
ACCESSION   DASTMT010000438 DASTMT010000000
VERSION     DASTMT010000438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4932)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4932)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4932
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2540185"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..399
                     /gene="ilvN"
                     /locus_tag="VFR00_10205"
     CDS             <1..399
                     /gene="ilvN"
                     /locus_tag="VFR00_10205"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008837017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HEU0159678.1"
                     /translation="SLTVSETEHAKHLSRFTVVTTGTAMVLEQIKSQLERLVNVHRVV
                     DLTALGEPLERELALVKVTGKGEKRIEALRMAEIFRANVIDASTEHFVFEVTGRISKI
                     EQFISIMTELGLLEVSRTGIAAIGRGPKGM"
     gene            517..1593
                     /locus_tag="VFR00_10210"
     CDS             517..1593
                     /locus_tag="VFR00_10210"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012456353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009027 - tartrate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0046395 - carboxylic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tartrate dehydrogenase"
                     /protein_id="HEU0159679.1"
                     /translation="MRNYRIAAIPGDGIGGEVIAAGVEALGVCAKRDGGFALAFDAFD
                     WGSRRYKETGAFMPANGLELIRNHDALLFGSAGAPDVPDHVTLWGLRLAICQPFDQYA
                     NVRPTRILPGITSPLRRVSGPELDWVIVRENSEGEYAGVGGRVHRGLPEEAATDVSMF
                     TRAGIVRIMRFAFALARSRPRKLLTVVTKSNAQRHGMVLWDEIALEISREFPDVSWDK
                     MLVDAMTMRMTLKPESIDTVVATNLHADILSDLAAALAGSLGIAPTANLNPERTFPSM
                     FEPIHGSAFDIMGKGIANPIGTFWSGVLLLEHLGEAPAARRLMRAIERVTADRNLHTP
                     DLGGTATTRQVTTAVCEALLGDNL"
     gene            1729..2820
                     /locus_tag="VFR00_10215"
     CDS             1729..2820
                     /locus_tag="VFR00_10215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006019619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEU0159680.1"
                     /translation="MQGEGGSVAQGMGQSVGSCMCGIPRALAARLGDAIPRRALARLL
                     GGLVALLLAGVPGGKAQQAYPTRPITVVVPAAAGGPTDTVARLIAEPMGRTLGQQIVV
                     ENVGGAGGTIGVGRVAKAGNDGYTLLVWHIGQSTAPSLYDNLRYDVFNDFDPIGRITD
                     VPMTIVGKTSLPPKDVAELISWLREKQDQVTYAHAGIGSAAHLCGLMLMSALNIKMNA
                     VVYRGTGPAMNDLVGGQFDVMCDQTTGTANQIKAGTIKGFAATTKTRIPLLAELPTLD
                     EAGVKGFEVSAWHALWGPKGLPKAVVEKLTAALQGALREPVVIERMAGLATVPVAQDL
                     ATPAALQTQLEAEVAKWGAVIRAAGVKAN"
     gene            complement(2823..3236)
                     /locus_tag="VFR00_10220"
     CDS             complement(2823..3236)
                     /locus_tag="VFR00_10220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0159681.1"
                     /translation="MSNRVRAIAIEAVTHFDVCCSGQHVEIGYLDHGGKPGRLYLSHA
                     SLGSLLLGLAAALQETGAGGSAELESACAPFLLDWRLTPERDEEVIVLSLKATNSVEV
                     AFRLPRPLALALGQALRNAGAAPPSPQARQPHVTF"
     gene            3408..4517
                     /locus_tag="VFR00_10225"
     CDS             3408..4517
                     /locus_tag="VFR00_10225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015669229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEU0159682.1"
                     /translation="MSWTIRGTLTLVLLCFASAGSAHSPEQAPHQLYGMGDFKLESGE
                     VIKDFSISYVTHGKLNANKSNAILMVTAIGGNHHRLDFMIGPGKALDPDRYFIVCTDA
                     IGNGLTTSPSNSSAQPRMQFPRFAMRDMVASQYRLVTEHLGLNHVLAVIGPSMGGMQT
                     LQWGVSHPEFMDALVALVPLAKTPAWTVSVLEASRKAIMLDPAWQGGNYTSPPERGVR
                     LWRDILLLAARTPEINRDQFPNNPLDVLPWLEQTETSLIKALDANDWLYQTWAYERHD
                     VGTTPPFNGDTAKALQSIRAKTLILLGTKDLLNPEWEPQEAAKHIRNAKVVTIRPTAV
                     TGHAAAGGAFAPDVEFINRETIGFLDVVSEAKKSN"
     gene            complement(4532..>4932)
                     /locus_tag="VFR00_10230"
     CDS             complement(4532..>4932)
                     /locus_tag="VFR00_10230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEU0159683.1"
                     /translation="LMVPFAHWITPEGVPKRFDTHFLLVAAPVSQLGAHDGCESTEGL
                     WIRPQQALADAEAGARTLVFATQMNLHKLARYPTVAAAIAAARSSPVVTVTPQAERLP
                     DGRRRLRIPAEAGYGLSEIVVTAPPAMPSP"
BASE COUNT          869 a         1620 c         1606 g          837 t
ORIGIN      
        1 tcgctgaccg tgtcggaaac cgagcacgcc aagcacctct cgcgtttcac ggtcgtgact
       61 accggcacgg ccatggtctt ggaacagatc aagagccagc tcgaacgcct cgtcaacgtg
      121 caccgcgtcg tcgatctcac cgccttgggc gagcccttgg agcgtgagct ggcgctggtg
      181 aaggtcaccg gcaagggcga gaagcgcatc gaggctttgc gcatggcgga aatcttccgc
      241 gctaacgtga tcgacgcttc aaccgagcat ttcgtgttcg aagttaccgg ccgcatctcc
      301 aagatcgagc agttcatttc catcatgacc gagctcgggc tgctggaggt ctcgcgcacc
      361 ggtattgccg cgatcggccg cggccccaag ggcatgtgag cgggtgctgg gcggcacccc
      421 cggttgcggc aaacttgcga agcccgaggt gacagcgcac gtccaagccg catagagtgc
      481 cggcagaaaa gcgtgcgaca tcgcccgagg gagcccatgc gcaactatcg aatcgctgcc
      541 attcccggcg acggcatcgg cggggaagtg atcgccgccg gcgtcgaagc cttgggcgtt
      601 tgcgccaaac gtgatggcgg ctttgcgctg gccttcgatg cgttcgattg gggctcgcgg
      661 cgctacaaag aaacgggcgc cttcatgccg gcgaacggtc ttgagctcat tcgcaaccac
      721 gatgccttgc tgttcggctc ggccggcgcc cccgacgtcc ccgaccatgt cacgctctgg
      781 gggctgcggc tggcgatttg tcaacccttc gatcaatacg ccaatgtccg gccgacgcgg
      841 atcctgcccg gcatcacctc gccgttgcgc cgcgtttcgg gtcccgagct cgattgggtg
      901 attgtgcgcg agaactcgga aggcgaatac gccggcgtcg gcgggcgggt gcatcgcggc
      961 ttgcccgagg aggcggccac ggatgtgtcg atgtttaccc gcgccggcat cgtgcgcatc
     1021 atgcgcttcg catttgccct ggcgcgttcg cgcccgcgca agctcttgac cgtggtcacc
     1081 aagtcgaatg cacagcgcca tggcatggtg ctgtgggacg aaattgccct cgagatctcg
     1141 cgcgagttcc cggacgtgag ttgggacaag atgctggtcg acgccatgac gatgcgcatg
     1201 accttgaagc cggaaagcat cgatacggtc gtcgccacca acctgcacgc cgatatcctg
     1261 tcggacttgg ccgcggcttt ggcgggttcg ctcggcattg cccccaccgc caacctgaac
     1321 cccgagcgca cttttccctc catgttcgaa ccgatccatg gctccgcctt cgatatcatg
     1381 ggcaaaggca tcgccaatcc gatcggcacc ttctggtcgg gtgtgctgct gctggaacac
     1441 ctcggcgagg ccccggcggc gcgccgtctg atgcgcgcca tcgagcgcgt cacggccgat
     1501 cgcaacctgc acacccccga cctcggcggc accgccacga cccgtcaggt gacaacggcg
     1561 gtctgcgagg ccctgctggg cgacaatcta tagttggccc cgcgcgcggg cggcagatcc
     1621 cggtgccacc ggcagcctgc cggcgatagc gcggtgggcc ggcgggccgg cgtggtcagc
     1681 cagcgccgcc tcgggctatc ctgcggcggc gatatcaggg agagcgatat gcaaggcgaa
     1741 ggcggcagcg ttgcccaggg gatgggccag agcgtggggt cttgcatgtg cggcatcccc
     1801 cgcgcgcttg cggcgcgcct gggggacgcg atcccgcgca gggccctggc acggctgctc
     1861 ggcgggcttg tcgccctgct gctggcgggc gtgccgggcg ggaaggcgca gcaggcctac
     1921 ccgacgcgcc ccattaccgt ggtggtgcct gccgccgccg gcggcccgac ggacacggtg
     1981 gcgcggctga tcgccgagcc gatggggcga acgcttggcc aacaaatcgt cgttgaaaat
     2041 gtcggcggcg ctggcggcac catcggcgtt ggccgggtcg ctaaggcggg caacgacggc
     2101 tatacgctct tggtttggca catcggtcag tcgacggcgc cctcgctcta cgacaacctg
     2161 cgctatgacg tcttcaacga tttcgatccg atcggacgca tcaccgacgt gccgatgaca
     2221 atcgtcggca agacatcgct gccgcccaaa gacgtcgctg aattgatcag ctggctcagg
     2281 gaaaagcagg accaggtcac ctacgcccac gccggcatcg ggtcagcggc gcacctgtgc
     2341 ggtttgatgc tgatgagcgc ccttaatatc aaaatgaatg ccgtcgttta ccgcggtacg
     2401 ggccccgcca tgaacgacct ggtgggcggc caattcgacg tcatgtgcga tcagaccacc
     2461 ggcaccgcca atcagatcaa ggcgggcacc atcaaaggtt tcgccgccac caccaaaacc
     2521 cgcattcccc tgctcgccga gttgcccacc ctcgacgagg cgggcgtcaa gggcttcgag
     2581 gtctcagcct ggcacgcgct ttgggggcca aagggcctgc ccaaggcggt ggtggaaaaa
     2641 ttgacggccg ccctgcaggg cgccttgcga gagccggtgg tgatcgagcg catggcaggt
     2701 ctggcgacgg tgcccgtggc gcaggatctc gcaacccccg cggccttgca gacgcagctc
     2761 gaagccgagg tcgcgaaatg gggcgccgtt attcgggcgg ccggggtgaa ggccaattga
     2821 actcagaacg tgacgtgcgg ctgccgcgcc tgcggcgagg gcggtgccgc gccggcgttg
     2881 cgcaaggctt ggcccagcgc cagggccaac ggcctcggca gccgaaaggc gacctcgacg
     2941 gagttggttg ccttcaggct gagcacgatg acctcctcat cccgctccgg cgtgagccgc
     3001 cagtcgagca agaaaggggc gcacgccgat tccaattcgg cgctgccgcc cgcgccggtc
     3061 tcttgcaggg ccgccgccag accgagcagc agcgaaccga ggctagcgtg cgacagatag
     3121 aggcgacccg gcttgccgcc gtgatccagg tagccgatct cgacgtgttg tcccgaacaa
     3181 catacgtcga agtgcgtaac ggcctcgatg gcaatggcgc gaacgcgatt ggacatgggg
     3241 acgctggcct cgtgtgctgc ttgccgcgcg cggcaggcgg ccacaacact atctattgtt
     3301 gacctgaaga gtcaagtttt tgccgtgtcg gcatgccgtt ttgacaaggc cggcggcgac
     3361 ttgttaatcg tcgctggttc ccggcggctc acaatgggag tgttgccatg tcttggacca
     3421 tccgcggcac gttgactcta gtcctgcttt gcttcgcttc ggccggctca gcgcattcgc
     3481 ccgagcaggc accgcaccag ctttacggca tgggagactt caagctggaa agcggcgagg
     3541 tgatcaagga tttctccatt tcctacgtga cgcacggcaa gctcaacgcc aacaagagca
     3601 acgctattct gatggtgacc gccattggcg gcaatcacca tcggctcgac tttatgattg
     3661 gccccggcaa ggcgctggat cccgatcgat atttcatcgt ctgcaccgat gcgatcggca
     3721 acggactgac cacttcaccc agcaatagta gcgcccagcc gcgtatgcag tttcccaggt
     3781 tcgccatgcg cgacatggtt gcctcgcagt accggctcgt caccgaacac ctcggcctca
     3841 accatgtcct ggccgtcatc ggcccgtcca tgggtggcat gcaaaccctg caatggggcg
     3901 tgtcgcatcc ggagttcatg gatgctctgg ttgcgctggt gccgctggcg aagacgccgg
     3961 cctggacggt cagcgtgctc gaggcctcgc gcaaagccat tatgctggat ccggcctggc
     4021 agggcggcaa ctacacgagc cctcccgagc ggggcgttcg cctgtggcgc gacattctcc
     4081 tcctggctgc ccgcacaccg gagatcaatc gcgaccagtt ccccaacaat ccgctcgatg
     4141 tgctgccctg gctcgagcaa accgaaacca gtctgatcaa agccttggat gccaatgatt
     4201 ggctgtatca gacctgggcc tatgagcgcc acgatgtcgg caccacgccg cccttcaacg
     4261 gcgacaccgc caaggcgctg caaagcatca gagccaagac gctgatcctg ctcgggacca
     4321 aggacctgct caacccggaa tgggagccgc aagaggccgc caagcacatc cgcaacgcca
     4381 aggtcgtgac catcaggccg acggcggtga cggggcacgc ggccgcaggc ggcgcctttg
     4441 cacccgacgt cgagttcatc aaccgcgaga ccattggctt tctcgacgtc gtgagcgagg
     4501 ccaagaaaag caactgaagg ggccgcccgc ctcagggcga cggcatggcc gggggcgcgg
     4561 tcaccacgat ctcgctcaag ccgtaacccg cttcggcggg aatgcgcagg cgacggcgac
     4621 cgtcgggcag gcgctccgcc tgtggcgtca cggtgacgac cgggctcgag cgcgccgcgg
     4681 cgatcgcggc tgcgacggtg ggatagcgcg ccagcttgtg caagttcatc tgggtggcga
     4741 acaccaaggt gcgcgcaccg gcttcggcat cggccaaggc ctgctgcggt cggatccaca
     4801 ggccctcggt ggactcgcac ccgtcgtgcg ccccaagctg gctgaccggc gccgccacca
     4861 gcagaaagtg ggtgtcgaat cgtttcggca cgccctccgg cgtaatccaa tgcgcgaacg
     4921 gcaccatcag at
//
LOCUS       DASTMT010000439         3012 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_4184660, whole genome shotgun sequence.
ACCESSION   DASTMT010000439 DASTMT010000000
VERSION     DASTMT010000439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3012)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3012)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3012
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4184660"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..409)
                     /locus_tag="VFR00_10235"
     CDS             complement(<1..409)
                     /locus_tag="VFR00_10235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014490498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEU0159684.1"
                     /translation="MGPLAGLRIVEMAGIGPAPFAATLLADMGADLIRVDRRTPTDLG
                     LPGREAKFNVLHRGRRSLAVDLKSKQGCEVVKRLAAKADALLEGFRPGVMERLGLGPE
                     ALLGLNPRLVFGRMTGFGQEGPMAQSAGHDINYI"
     gene            572..2791
                     /locus_tag="VFR00_10240"
     CDS             572..2791
                     /locus_tag="VFR00_10240"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU0159685.1"
                     /translation="MSQLHDLSGRLPALLQMLQGRLLRAGRLRLALGRRKPRAAPPAP
                     GLPVEFERFVRIGERLERGLEELKDIQWQIRENEARYRDLLDNQADVILRRDAQGRLT
                     FVNRAFCGVFGLEREDVLGRPFAHRVLAGDAVLPLTAGGALRQQRYVQEIDTAAGPRW
                     FEWEEHAVPGSETSVAEVQCLGRDITEERRAEAELKEARKQAVAANRAKSRFLAAMSH
                     EIRTPMNGILGMTQLLCETELAPEQQTYARAIDQSARTLLILIDEILDFSKIEADKLQ
                     LNCAGVAIEDCVQGVVELLAPKAYEKGIDIAWAVDPKLPRHLLGDEVRLRQIITNLVG
                     NAIKFTDAGGVLITVAPAAHAGKPTAGEVAVAITVEDTGIGIAAEALAALFGEFEQAE
                     VAFERRQGGTGLGLAISRRLARAMAGDIQVSSRPGCGSTFVATVRLKPAEQPLAPRPA
                     RAGTASMPHVLLALDRPVERRALRLALEGAGVPLEEGPVLSAADLIEAAAGLGEPFTT
                     LVVDGRCGVMTATSLLSAARQAAGAQGVHGIVVLNTAAKADFAEFRGAGFATYLVRPV
                     RPRSLLTHLGAGSVQPSEPAGRTKATAEPSWLAGPPRVLLVEDNDINALLARRMLEKV
                     GCVASLCGNGREAVDAVRRVIAGGEQPYDLILMDVHMPVLDGLEATRSIKSLWGAVAD
                     GTATSAPIVALTANAFDDDRRRCLEAGMDDYLAKPFDREDLYRLLTRWCSSGRTRAA"
     gene            complement(2805..>3012)
                     /locus_tag="VFR00_10245"
     CDS             complement(2805..>3012)
                     /locus_tag="VFR00_10245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011974244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HEU0159686.1"
                     /translation="LKDTANVLVMPAFHSASISTKMLKELGGATLIGPVLAGLTHSVQ
                     ICPLNAKDSDVVNMAALAAYDVNT"
BASE COUNT          498 a          978 c         1010 g          526 t
ORIGIN      
        1 agatgtagtt gatgtcgtgg ccagcgcttt gcgccatcgg tccctcctga ccgaaaccgg
       61 tcatgcggcc gaacacgagc ctgggattga gcccgagcag cgcctccggc ccaagcccga
      121 ggcgctccat cacgcccggc ctgaagcctt cgagcagcgc gtcagccttt gctgcgaggc
      181 gctttaccac ctcgcagcct tgcttgctct tgaggtcgac ggccagcgag cgccggccgc
      241 gatgcaacac gttgaatttc gcttcccgcc cgggcaggcc caggtctgtt ggcgtgcgtc
      301 gatcgacccg gatcaggtca gcacccatgt ctgcaagcag cgtcgccgcg aacggcgccg
      361 ggccaattcc ggccatctcg acaatgcgca atccggccaa aggacccatc gtggttacct
      421 cttggttggt cgccgtgccg aaacgcaccg tcggcggcaa gcggggcgtg cggcggcggc
      481 aggcgtgaac aatccgggta aacgtttcta aatctggatg tggttctcat gcacgataaa
      541 gccgtcaacg ataaatgatc gggcgtcaag ggtgtctcag ctgcacgacc tgtcgggccg
      601 gctgccggct ttgctgcaaa tgctgcaagg gcgactgctt cgtgctggcc gcctgcgcct
      661 cgcgctcggc cggcgtaagc cacgcgctgc cccgccggcg cctggcctgc ccgtcgagtt
      721 cgagcgcttc gtgcgcattg gcgagcgtct cgaacggggg ctggaggagc tcaaggatat
      781 tcagtggcaa atccgggaaa acgaggcgcg ttaccgggac ctgctcgaca atcaagccga
      841 tgtgatcctg cggcgtgacg cccagggccg cttgaccttc gtcaaccgcg ccttctgcgg
      901 cgtgttcggt ctggagcgcg aggacgtgct cggccggccg tttgcgcatc gggtgttggc
      961 cggcgatgcc gtgctgccac tgacagcggg cggcgcgctg cgccagcagc gctacgtgca
     1021 ggagatcgac acggcggcgg gcccgcgctg gtttgaatgg gaagagcatg ccgtgcccgg
     1081 tagcgagacg tcggtcgcgg aagttcagtg tctcggccgc gacatcacgg aagagcgtcg
     1141 cgccgaggcg gagctcaagg aggcgcgcaa gcaggcggtg gcagccaatc gtgccaagtc
     1201 acgcttcctc gctgccatga gtcacgagat tcgcacaccg atgaacggca ttctcggcat
     1261 gacccagttg ttgtgtgaga cggagctagc gcccgaacaa cagacctacg cccgagccat
     1321 cgatcaatcc gcccgcaccc tgctgatcct gatcgacgag atcctcgatt tctccaaaat
     1381 cgaggcggac aaactgcagc tcaactgcgc cggcgtggca atcgaggact gcgtgcaagg
     1441 cgtggtcgag ttgctcgcgc ccaaggccta cgaaaaaggg atcgacattg cctgggccgt
     1501 cgacccgaaa ctgccgcgtc atctgctcgg cgacgaggtg cgcctgcgcc agatcatcac
     1561 caatctggtg ggcaatgcca tcaagttcac cgatgcgggt ggtgtgctga tcaccgtcgc
     1621 accggcagcg catgcgggca agcccactgc gggcgaggtc gcggtggcga tcacggtgga
     1681 agacaccgga atcggtatcg ctgccgaggc gctggccgct ttgttcggcg agttcgagca
     1741 ggccgaagtt gcattcgagc gccgtcaggg tggcaccggg ttgggccttg caatctcgcg
     1801 gcgtctggcg cgcgccatgg cgggcgacat tcaggtcagc agccgaccgg gctgtggctc
     1861 aacgttcgtg gcaactgtgc gcctcaagcc tgccgagcag ccgctggcac ctcggcccgc
     1921 ccgcgccggc acggcatcga tgccgcacgt gctgttggcg ctggatcgac ccgtcgagcg
     1981 ccgcgctctg cgcttggcgc tcgaaggcgc cggcgtcccg ctcgaagaag gccccgtttt
     2041 gagcgccgcc gacctgatcg aggccgccgc cggcctcggc gagcctttca ccaccttggt
     2101 ggtcgatggc cgctgcggtg tgatgacggc cacgagcctg ttgtccgccg cccgacaggc
     2161 ggctggcgcc cagggcgtgc atggcatcgt cgtgctcaac accgccgcca aagccgattt
     2221 tgccgagttt cgcggagcgg gcttcgctac ttatcttgtg cgccccgtgc ggccgcggtc
     2281 gctgctgacc cacctcggcg ccggttcggt tcagccaagc gagccggccg gccgcaccaa
     2341 ggccacggcc gagccgagct ggcttgccgg cccaccccgg gtcctattgg tggaagacaa
     2401 cgacatcaac gccttgctgg cgcggcgcat gctggagaag gtcggttgcg tggcgagcct
     2461 gtgcggcaac ggccgcgaag cggtcgatgc ggtgcgccgg gtgatcgccg gcggcgagca
     2521 accctatgac ctcattctca tggacgtgca tatgccggtg ctggatggat tggaggccac
     2581 gcgctcgatc aagtcgcttt ggggtgcggt cgccgacggg accgcgacct cggctccgat
     2641 cgtggcattg accgccaacg ccttcgatga tgatcgtcgc cgctgcctgg aggcggggat
     2701 ggacgactat ttggccaagc ccttcgatcg cgaagatctc taccggctgc tcacacgctg
     2761 gtgcagcagc gggcgcacgc gcgcggccta gtcggtgtcg gggctcaggt gttcacgtcg
     2821 taggcggcca aggcggccat gttcacgaca tcactatcct tggcattcaa cggacaaatt
     2881 tgcaccgagt gcgtaaggcc cgccaacacc ggcccgatca acgtcgctcc gcccagttct
     2941 ttcagcatct tggtcgagat ggaggcggaa tggaaagccg gcatcaccag cacgttggcc
     3001 gtatccttca gg
//
LOCUS       DASTMT010000440         4176 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_2221104, whole genome shotgun sequence.
ACCESSION   DASTMT010000440 DASTMT010000000
VERSION     DASTMT010000440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4176)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4176
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2221104"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..293)
                     /locus_tag="VFR00_10250"
     CDS             complement(<1..293)
                     /locus_tag="VFR00_10250"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU0159687.1"
                     /translation="MTAPLLEVRDLRTHFFNEDGVTRAVDGLSFAVAPQETVGIVGDS
                     GCGATVTALSILQLLPPRLGRIIGGRILFEGTDLTRLSEAQLREVRGNRIGMI"
     gene            complement(303..1172)
                     /locus_tag="VFR00_10255"
     CDS             complement(303..1172)
                     /locus_tag="VFR00_10255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014531748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0159688.1"
                     /translation="MTVAAAEIAYVPRRSGLWHFVRRNPTLVGGMVVLVLVALAALAA
                     PLIAGDPIAAKPVNRLRAPSQINWLGTDQLGRDVWARTVYGARISLVVGLAVASIAMA
                     IGLLIGLLAGFSRTVESVVMRLMDGLMAIPAILLAIAFVSLSKASVGIVVAAIALPEI
                     PRVVRLVRSVVLSVREQPFVEAAVASGTRLPKILVRHILPATVPPLIVQATYVAASAI
                     LIEAALSFLGAGTPPEIPTWGNMIASSRVYLGLAPWTIFGPGLALALVVLAVNLVGDG
                     LRDRLDPRIARRM"
     gene            complement(1174..2115)
                     /locus_tag="VFR00_10260"
     CDS             complement(1174..2115)
                     /locus_tag="VFR00_10260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018315994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0159689.1"
                     /translation="MLAFIIRRLFSTLLVMGLVGVFIFLLLHLSPGDPAAIIAGDNAT
                     PAQIEAIRTSLGLNDPLHLQFGRWAVRVLNGDLGISIFSNVPVATLIGQRVWPTLSLA
                     LTTIVLAVVLALTAGVLAAWKAGSLLDRLVMILSVVGFSVPVFVVGYLLIYVFAITLR
                     WLPVQGYTPLSEGLWPWASHLVLPSLALGLAYVALIARITRTAMLDVLAEDYMRTARA
                     KGVATGPMLFKHALKSAGVPIVTVVGIGIALLIGGVVITETVFNLPGIGRLVVDAISK
                     RDYPIIQGVILLFSGVYVIINLLVDLSYTLLDPRIRY"
     gene            complement(2205..3821)
                     /locus_tag="VFR00_10265"
     CDS             complement(2205..3821)
                     /locus_tag="VFR00_10265"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0159690.1"
                     /translation="MTRRATTSRRQFLQTGLAAGAAVAAPGRLTAQSAPPAAKTIRAV
                     MHGDLTVLDPIWTTANMSAYHGAMLYDTLFGIDANLDTKPQMVSKWGASDDKKTWTFE
                     LRDGLKFSDGSAVTSEDVIASLKRWAARDGAGQHMMQRVADIAKKDDKTFQFILKEPY
                     GYLVDALGKTSTPLCYIMRKKEAETNPMEKITAYVGSGPFIFNETETRQGVRYVYDKN
                     PNYVPRAEPASGLAGGKVAKVDRIMFENMADSQTALAALQAGEIDFYETPPVDLIGQL
                     EGDSNLTLEVLNKTGNIGWLRMNFLHPPFNNVDARRAMLYLLDQEDFVKATFGNPKYY
                     KKCPSNFGCGVPMENDANTGWFKEAPNPAKAKDYLQKSGYKGEPILLMQATNIDFMRN
                     AADLVAGALRSIGANVQVASSDWGGVVNRRAVKAPPDQGGWNLFITWADGNAVSNPIA
                     LAGHAATGEKAWFGWPSDAKNEELRDKWALAGSLAEKQQIARELQENAWNFVPHVWLG
                     QWVAPTARRKNLTGMLAVPGVVPFWNVQKA"
BASE COUNT          751 a         1318 c         1430 g          677 t
ORIGIN      
        1 aagatcatgc cgatgcgatt gccgcgaacc tcgcgcaact gtgcttcgct cagtctggtg
       61 aggtcggtgc cctcaaacag aatgcggccg ccgatgatgc gccccagacg ggggggcagc
      121 agctgcagga tcgataaggc cgtgaccgtc gcgccgcagc cggagtcgcc gacgatgccc
      181 accgtctcct gcggcgccac cgcgaaggaa aggccgtcga cggcgcgcgt gacgccgtcc
      241 tcgttgaaaa agtgcgtcct gagatcccta acctcgagca gcggcgcggt catccctggc
      301 gctcacatcc ggcgggcgat gcgcggatcg aggcggtcgc gcagcccatc gccgacgaga
      361 ttcaccgcca ggaccaccag cgccaaggcg agacccgggc caaaaatggt ccagggcgcc
      421 aagcccaggt agacgcggct cgaggcgatc atattgcccc aggttggaat ttccggcggc
      481 gtaccggcgc cgagaaagga taaggccgct tcgatcagga tggcggaggc cgccacgtag
      541 gtcgcctgca cgatcagcgg cggcacggtg gcgggcagaa tgtgccggac gaggatcttg
      601 ggcagtcgcg tgccgctggc caccgccgcc tcgacgaatg gctgctcgcg gaccgacagc
      661 accaccgagc gcaccagccg caccaccctt gggatctcgg gcagggcgat ggcggcgacg
      721 acgatgccga cgctcgcctt cgacagcgag acgaaggcga tggcgagcag gatcgcgggg
      781 atggccatca gcccatccat caggcgcatc accacgcttt ccaccgttcg gctgaagccg
      841 gcgagcaggc cgatcagaag gccgatggcc atggcgatgc tggcgaccgc aaggccgacg
      901 accaaggaaa tgcgggcccc gtaaacggtg cgggcccaga cgtcgcggcc gagctgatcg
      961 gtgcccagcc agttgatctg cgagggggcg cgcagccggt tgaccggctt ggcggcgatg
     1021 ggatcgccgg cgatcagggg ggcggcgagg gcggccagcg ccaccagcac gagcacgacc
     1081 atgccgccga ccagggtcgg attgcgccgc acgaagtgcc acaagccgct gcggcgcggc
     1141 acataggcga tttctgcggc ggcaaccgtc atgtcagtag cggatccggg gatcgagcaa
     1201 ggtgtaggaa aggtcgacca gcagattgat gatcacgtag accccggaga acagcaggat
     1261 cacgccctgg atgatcggat agtctcgttt ggaaatggcg tcgaccacca gacggccgat
     1321 acccggcagg ttgaagaccg tttccgtgat cacgaccccg ccaatcagca aggcaatgcc
     1381 gatacccacc accgtgacaa tcggcacgcc ggcgcttttc agggcgtgct tgaacaacat
     1441 ggggccggtg gccaccccct tggcccgcgc ggtgcgcatg tagtcctcgg ctaatacgtc
     1501 gagcatggcc gtgcgcgtga tgcgcgcaat caacgccaca taggccaggc cgagggcgag
     1561 ggagggcagg accaggtgac tagcccaggg ccagaggccc tccgagaggg gcgtgtagcc
     1621 ctgaacgggc agccagcgca gggtgatggc gaacacataa ataagcagat agccgacgac
     1681 gaaaaccggc acggaaaagc cgaccaccga caagatcatc accaggcgat cgagcaggct
     1741 tccggctttc caggccgcaa ggacgcccgc ggtcagggcg agcacgaccg cgagcacgat
     1801 ggtggtgagc gccagcgaga gggtgggcca gacccgttgg ccaatcaggg tggcgaccgg
     1861 cacgttggaa aagatcgaga tgccgagatc tccgttgagg acgcgcaccg cccagcggcc
     1921 gaactgcaga tgcagcggat cgttgaggcc aaggctggtg cggatggcct cgatctgggc
     1981 gggcgtggcg ttgtcgccgg cgatgatggc ggcggggtcg ccaggcgata gatgcaggag
     2041 caggaagatg aatacgccca ccagccccat gacgaggagc gtggagaaca ggcggcggat
     2101 gatgaaggcc agcatgtccg tcgattacgc ctgcctcgat gcccttgccg gcgggacgcg
     2161 ttgccaaagc ttctggcgcg cgccgccggg cgcgcaccgg tcgctcacgc cttttgcaca
     2221 ttccagaacg gcaccacgcc gggcacggcc aacatgcccg tcaggttctt gcggcgcgcg
     2281 gtcggggcga cccattgccc gagccagacg tgcggcacga agttccaggc gttttcctgc
     2341 agctcgcggg cgatctgctg tttctccgcc aggctgcccg cgagcgccca cttgtcccgc
     2401 agctcctcgt tcttggcgtc cgatggccag ccgaaccagg ccttttcgcc ggtcgccgcg
     2461 tggccggcaa gtgcgatcgg attgctgacg gcattgccgt ccgcccaggt gatgaacagg
     2521 ttccagccgc cctgatcggg tggcgccttc accgcccggc ggttgacgac accgccccag
     2581 tcggaggagg ccacctgcac attggcgccg atcgagcgca gcgcgcccgc gacgagatcg
     2641 gcggcattgc gcatgaaatc gatgttggtc gcctgcatca acaggatcgg ttcgcccttg
     2701 tagcccgact tctgcaggta gtccttggcc ttcgccggat tgggcgcctc tttgaaccag
     2761 ccggtattgg cgtcgttctc catcggcacc ccgcagccga agttggacgg gcactttttg
     2821 tagtatttgg gattgccgaa ggtcgccttg acgaaatcct cttggtcgag gaggtagagc
     2881 atggcacggc gcgcatcgac gttgttgaac ggcggatgca ggaagttcat gcgcagccaa
     2941 ccgatgttgc ccgtcttgtt caacacctcg agcgtgagat tgctgtcgcc ctcgagctgg
     3001 ccgatgagat cgacgggcgg ggtctcgtaa aaatcgatct cgccggcctg cagggcggcg
     3061 agcgcggttt ggctgtcggc catgttctcg aacatgatgc gatcgacctt cgccaccttg
     3121 ccgccggcaa gtcccgaagc cggttccgca cgcggcacgt agttggggtt cttgtcgtag
     3181 acgtagcgca cgccctgcct ggtctcggtc tcattgaaaa tgaaggggcc cgacccgacg
     3241 taggccgtaa tcttttccat cgggttcgtc tcggcttcct tcttgcgcat gatgtagcac
     3301 agcggcgtcg aggtcttgcc cagcgcgtcg accaaataac cgtagggctc tttcagaatg
     3361 aactggaagg tcttgtcgtc cttcttggcg atgtcggcga cacgctgcat catgtgctgg
     3421 ccggcgccgt cgcgcgccgc ccagcgtttg agcgaggcga tgacgtcctc ggaggtcacc
     3481 gccgagccgt cgctgaactt gagcccgtcg cgcagctcga aggtccaggt tttcttgtcg
     3541 tccgacgcac cccatttgct caccatctgc ggtttggtat cgagattggc gtcgatgccg
     3601 aacagcgtgt cgtagagcat ggcgccgtga taggcgctca tgttggccgt ggtccaaatt
     3661 gggtcgagca cggtgaggtc accgtgcatg accgcgcgga tggtcttggc ggcagggggc
     3721 gccgactggg ccgtcagccg accgggtgcc gccacggcgg cgcccgccgc aagaccggtt
     3781 tgcaggaatt ggcggcgcga tgtggtggcg cggcgtgtca tcggcatttc tccttgggct
     3841 gggcccaagc aaggcccgcg gtcatctctt caaccgtggc cggcgcaacc gacccggcgc
     3901 gattaacgca tgcgtgcgcg gccgcggcaa gaggaggcca ggcgccaccg cccgcccagc
     3961 gcgctgcgat tgccggcggc ggacgaagcg gcgcgagacg gcgccggcgt gcgcgggcct
     4021 tcctttgccg aggcggctcc actctgatgt cccaagtcaa gcactccgtc cacacgttgt
     4081 cgacaccggt ccctctacgg ggcggcgtgg tgtctgaatg tatcgtgcag tggcggaagc
     4141 gaggcccgaa gacgtcggtg atcaagctct gatgcg
//
LOCUS       DASTMT010000441         2551 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_2542658, whole genome shotgun sequence.
ACCESSION   DASTMT010000441 DASTMT010000000
VERSION     DASTMT010000441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2551)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2551)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2551
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2542658"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1129
                     /gene="argJ"
                     /locus_tag="VFR00_10270"
     CDS             <1..1129
                     /gene="argJ"
                     /locus_tag="VFR00_10270"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HEU0159691.1"
                     /translation="LMAAVLEPGTSVAGVLTRSKTCSAPVLWCRDNLEHGRARALVVN
                     SGNANAFTGKKGSEATRLTAEAMGEAVGCPASEVYVASTGVIGEPLDAHRFAHLLGDL
                     VRSAAADQVEIAARAIMTTDTYPKLATRSAEIAGVPVSINGFCKGAGMIAPDMATMLC
                     FLFTDAALPPKVLQELLVRHVRTTFNCMTIDGDTSTSDTCLLFATGAAAKRGQPPIAS
                     AQERSLAVFEAALHDLLRDLAIQVAKDGEGLSKFVTIEIAGAESWEAARRIGLACANS
                     PILKAAIFGEDPNWGRLVMAIGKAGEAADRDKLSIWFGPHEVARNGQRAPDYVEQAVA
                     DYMKGREILIRADVGVGAAEATVWTCDLTHDYISINADYRS"
     gene            1298..1702
                     /locus_tag="VFR00_10275"
     CDS             1298..1702
                     /locus_tag="VFR00_10275"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018207851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(deoxy)nucleoside triphosphate
                     pyrophosphohydrolase"
                     /protein_id="HEU0159692.1"
                     /translation="MGSDILLVVACALIDADGRVLLAKRPSGRPLAGLWEFPGGKVEP
                     GETPEAALIRELEEELGIRVPPKCLAPFTFASHGYAAFHLLMPLYVCRKWEGSVKAQQ
                     GQELVWVRAKRLADYAMPPADEPLKAMLRDLL"
     gene            1741..1956
                     /locus_tag="VFR00_10280"
     CDS             1741..1956
                     /locus_tag="VFR00_10280"
                     /inference="COORDINATES: protein motif:HMM:NF016828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HEU0159693.1"
                     /translation="MTIVKALRLKRSALGGFLADRGGATSLEYGLIAVLVAVGMLAGL
                     KALGAGNSSSWSSTSNKITGAMQQRAP"
     gene            complement(1973..>2551)
                     /locus_tag="VFR00_10285"
     CDS             complement(1973..>2551)
                     /locus_tag="VFR00_10285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012110647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEU0159694.1"
                     /translation="LVVSALALQLVNDLPGTLLQVRQALKPDGLLLAAILGGATLHEL
                     RTALFLAEEEVEGGASPRVAPFADVREVGSLLLRAGFALPVVDAESVTVTYPDALALM
                     RELKAAGVANALRARRRRPLRRETLARALHHYAQRFGLPTGRVPATFEIVTLTGWAPH
                     ESQQQPLRPGSAAVRLADALGTVERRLGERPE"
BASE COUNT          438 a          819 c          858 g          436 t
ORIGIN      
        1 cctgatggcg gccgtgctgg agcccggcac gagcgtggcc ggggtgctca cccgctccaa
       61 aacctgctcg gctccggtcc tgtggtgccg ggacaatctt gagcacggcc gtgcccgggc
      121 cctcgtcgtc aattccggca acgcgaatgc cttcaccggc aagaagggca gcgaggccac
      181 ccgtctaacc gccgaagcga tgggcgaggc ggtcggatgt cccgccagtg aggtctatgt
      241 cgcctccaca ggcgtgatcg gtgagccgct cgacgcgcac cgtttcgcgc acctgctcgg
      301 cgatcttgtg cgctccgccg cggccgatca ggtcgagatt gccgcccgtg ccatcatgac
      361 cacggacacc tatcccaagc ttgccacgcg atcggccgaa atcgccgggg tgccggtgag
      421 catcaacggg ttctgcaagg gcgcaggcat gattgcaccc gacatggcga cgatgctgtg
      481 cttcctcttc accgacgcgg cgctgccgcc aaaggtgtta caagagcttt tggtgcggca
      541 cgtgcgcacg acgttcaact gcatgaccat cgacggagat acttcgacca gcgatacctg
      601 cctgttgttt gcgaccggtg cggcggccaa gcgggggcag cctccgattg cgagcgccca
      661 agagcgttcc cttgccgtct tcgaggccgc gctgcacgat ctattgcgcg atttggccat
      721 ccaggtcgcc aaggacggcg agggcctctc caagttcgtg accattgaaa ttgccggggc
      781 ggaaagctgg gaagccgcgc gccggattgg actggcgtgc gcaaactcgc ccattttgaa
      841 ggcggctatt tttggcgagg atcccaattg gggtcgcctc gtcatggcga tcggcaaggc
      901 cggcgaggcc gctgatcgcg acaagctatc gatctggttc ggcccgcacg aggtcgcccg
      961 caatggccag cgtgctcccg actacgtcga gcaggcggtg gccgactaca tgaaggggcg
     1021 cgagattctg atccgcgccg acgtgggggt gggagcggca gaggccaccg tctggacctg
     1081 tgatttgacg cacgactaca tctccatcaa cgctgattat cgcagctagc gcaagccgcg
     1141 gcgaggcggg cggcacctca ccggtcggcc gccgtgccaa ggcgcctgcg cgggcgccgg
     1201 cacgagcccc gcaggatggc cggtgcgcgc tttgccaatc cttaaccccc ctctctgccg
     1261 aagtgttaac atctcatccg taggctggag cataagcatg ggctcggaca ttctgcttgt
     1321 cgttgcctgc gcgctgatcg acgccgatgg gcgtgtgctg ctggccaagc gtccctcggg
     1381 acggccgctc gcggggctct gggagttccc gggcggcaag gttgagccgg gtgagacacc
     1441 cgaagcggcc ctcattcgcg agctcgaaga agagctcggg attagagtgc cgccgaagtg
     1501 cctcgcgccc ttcaccttcg ccagccacgg ctatgcggcg ttccatctgc tgatgcctct
     1561 ttatgtgtgc cgcaagtggg agggatcggt gaaggcgcaa cagggacagg agcttgtctg
     1621 ggttcgcgcg aagcgacttg ccgactacgc gatgccgccg gccgatgagc cattgaaagc
     1681 catgcttcgg gatctgctgt gacccgcgca ggcttggggg agtttggaga ggctttgccc
     1741 atgacgatcg ttaaggcttt gcgcctgaag aggagcgcct tgggcggctt cctcgccgac
     1801 cgcggtggtg cgacctcgct cgaatatggc ctcattgccg tgctcgtggc ggtcgggatg
     1861 ctcgccggac tcaaagccct cggtgccggc aacagcagca gttggagcag taccagcaat
     1921 aaaatcactg gagcgatgca gcagcgcgcg ccttgaagac acctgccggc tttcactcag
     1981 gccgctcgcc aagccggcgc tccaccgtgc caagcgcatc cgcgagccgg accgccgccg
     2041 acccgggtcg caacggttgc tgttgacttt cgtggggggc ccagccggtc agggtgacaa
     2101 tctcaaaggt ggcgggcacg cggccggtgg gcaggccgaa acgctgggcg tagtggtgca
     2161 aagcacgggc gagcgtctct cggcgcagcg gccggcggcg gcgggcgcgc aaggcgttgg
     2221 cgacccccgc agccttgagc tcgcgcatga gggccagggc atccggatag gtcacggtca
     2281 cgctttcggc gtcgacgaca ggcagcgcga aacccgcacg cagcaaaagc gaaccgacct
     2341 cccttacgtc tgcaaatggc gcaacgcgcg gactggcacc cccttctact tcctcctcgg
     2401 ccaaaaatag cgcggtgcgc aattcgtgca gggtcgcacc tcccaagatc gcggccaaca
     2461 gtagcccgtc cggcttaagc gcctggcgga cctgcagcag cgtcccgggc aggtcattga
     2521 cgagttgcag ggctagggcc gagacgacaa g
//
LOCUS       DASTMT010000442         1885 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_2873393, whole genome shotgun sequence.
ACCESSION   DASTMT010000442 DASTMT010000000
VERSION     DASTMT010000442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 1885)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1885)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1885
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2873393"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..739
                     /locus_tag="VFR00_10290"
     CDS             <1..739
                     /locus_tag="VFR00_10290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0159695.1"
                     /translation="LERADGLRAAIVQTAYKVKVKGIATPSRLRSALAALALQRAFGN
                     QISATLTGRTALPPRAGRLLAAQLCRSPRDHGTDTRLIAALAAEHLGAKASDLEALRR
                     AVFYRFFAGLVETGEAALRPPPRPPAKREAVAPPGPAPLAVIGGRPDLPGFVLEVRRH
                     AAAHAHGWDGNRKAYICHVWRALAQARADWGLSEIEFKCMLLEAHRAGQLALANADLK
                     DHRSMKDVQDSAVVYKNAVFHFVRADG"
     gene            786..>1885
                     /locus_tag="VFR00_10295"
     CDS             786..>1885
                     /locus_tag="VFR00_10295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU0159696.1"
                     /translation="MEVESAPDLDRLSAAFEHSITWEDDIWRADPVDVEIVHAHARRK
                     FNELLDTVTSAQGAQGQARILLFHGQSGAGKTHLIRALRTSAHRSGKAYVGYAQMTPD
                     VANYADYYLRRLINSLEKPYDPDTGGETALQRLTRRLTEAAEPLDTTALDKLRDAKLS
                     DAQLAKLVLALADQIVAAAKFAGQPLDINIVRALLYLQRADPRIDQRVRQYLYGRELT
                     DLSAEAVAVLDPNRGEGRAFEIIESLGRLMWIVDGAALVISIDQVEDLRFFDDYHERF
                     QKAARDLIQIANRVPTSIVLLSCLGDFYGKVREVLAQSYIDRIEKAGPVSLKEARTAE
                     EAQLIIAKRMQADASRSDIDPLLFFGPQFFEE"
BASE COUNT          318 a          647 c          601 g          319 t
ORIGIN      
        1 cctggagcgg gccgacgggc tgcgtgcggc catcgtccag accgcctaca aggtgaaagt
       61 caagggcatt gccacgccgt cgcggctgcg ctccgccctg gcggccctgg ccctgcagcg
      121 cgctttcggc aaccagatca gcgccacctt gacgggcaga actgcgctgc cgcctcgggc
      181 gggacgtcta ttggccgccc agctctgccg gtcgccgcgc gaccacggca ccgatacgcg
      241 tctgatcgcg gcgctggcgg ccgagcacct cggcgccaag gcgagcgacc tggaagcact
      301 gcggcgtgcc gtcttttatc gtttctttgc cggcctggtc gagaccggcg aggcggccct
      361 gcgcccgccg ccgcgcccgc cggcaaaacg ggaagcggtt gcgcctcccg ggccggcgcc
      421 gctcgccgtc atcggcggcc ggccggacct cccaggcttc gtgctcgaag ttcgccggca
      481 tgccgccgcc cacgcgcacg gttgggacgg aaaccgcaag gcctacatct gccatgtctg
      541 gcgtgcgctc gcccaggcgc gcgccgactg gggcctgtcc gagatcgagt tcaagtgcat
      601 gctgctggag gcgcaccgcg ccggacaatt ggcgctggcc aacgccgatc tcaaggatca
      661 caggagcatg aaagacgtgc aggactctgc cgtcgtctac aagaacgcgg tgttccattt
      721 tgtcagggcc gatggctgag gccgcggctc gccggtacct aacgtcagct tgaggtgaca
      781 tgcgcatgga ggttgagtcc gcgcccgatc tggaccgcct gtcggcggct ttcgaacact
      841 ccatcacctg ggaggacgac atttggcgcg ccgatcccgt cgacgtcgag atcgtgcacg
      901 cccatgcgcg gcgcaagttc aacgaattgc tggacacggt gacctcggcg cagggggcgc
      961 aggggcaagc gcgcatcctg ctgtttcacg gccagtcggg cgccggtaaa acccatctca
     1021 tccgcgcctt gcgcacgtcc gcgcatcgtt cgggcaaggc ctacgtcggc tacgcgcaaa
     1081 tgacgccgga cgtggcaaac tacgccgatt actatctgcg ccgtctcatc aactcattgg
     1141 aaaagcccta tgatccggac acgggcggcg aaaccgccct gcaacggctg acgcggcgcc
     1201 tgacggaggc ggccgagccc ctcgatacga ccgccctcga caagctgcgc gacgccaagc
     1261 tcagcgacgc ccaacttgcc aagctggtgt tggcgctggc ggatcagatc gtggccgcag
     1321 ccaagttcgc tggccagccg ctcgatatca atatcgtgcg agccctgctc tatctgcagc
     1381 gggcagaccc gcgcatcgac cagcgcgtgc gccagtacct atacggtcgt gagttgaccg
     1441 atctctccgc cgaagccgtg gccgtgctcg atcccaatcg aggtgagggc cgcgccttcg
     1501 aaatcatcga gtcgctcggg cgcttgatgt ggattgtcga tggcgccgcc ctcgtcatct
     1561 ccatcgatca ggtggaggat ctgcgcttct tcgacgatta ccacgagcgc ttccagaagg
     1621 cagcccggga cctgatccag atcgccaacc gcgtgccgac ctcgatcgta ctgctgtcct
     1681 gcctcggcga cttctacggc aaggtgcggg aagtgttggc gcaatcctac atcgatcgca
     1741 ttgaaaaggc gggcccggtc tccctcaagg aggcgcgcac cgccgaggaa gcgcagctca
     1801 ttattgccaa gcgcatgcag gccgacgcca gccgcagcga catcgaccct ttgttgttct
     1861 tcggcccgca attcttcgag gagtt
//
LOCUS       DASTMT010000443         6987 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_3531476, whole genome shotgun sequence.
ACCESSION   DASTMT010000443 DASTMT010000000
VERSION     DASTMT010000443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 6987)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6987)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6987
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3531476"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..311
                     /locus_tag="VFR00_10300"
     CDS             <1..311
                     /locus_tag="VFR00_10300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013947736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0159697.1"
                     /translation="AIAEAMVRNARPGSIIVSHANGRGYHTAEALPLAIPLLRAKGFS
                     FVTVSELLAAGKAVMAETCYDAHPGDTDRYDFLRGHTPTASPSWEPTTHVRAGPSLPR
                     "
     gene            280..801
                     /locus_tag="VFR00_10305"
     CDS             280..801
                     /locus_tag="VFR00_10305"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU0159698.1"
                     /translation="MSEPAPPFRAERYALRSLGDGRTLALAPFSAAAADRLGPATAAI
                     GPWAHYGFDGDKLAAGFKRTEEGIHRFQVECDGDLAGALIVVCPWLAGPYLQMLAIQP
                     AYQKHGIGARILAWYEAEARGAFRNLWLCVSGFNVDAQRFYIAHGYTQVASLDGLMRE
                     GDVELLMRKRLQP"
     gene            complement(841..1713)
                     /locus_tag="VFR00_10310"
     CDS             complement(841..1713)
                     /locus_tag="VFR00_10310"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011085343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HEU0159699.1"
                     /translation="MRDPQALMRTEQLAARLGQANLRIYDCTTYLEAAPAGSDQPYVV
                     VPGRRSFAEAHIPGADFLDLQGEFSDNASPLRFMLAAPASLEAAFGRHGVGAGAQVVL
                     YSLGTSMWATRFWWMLKSLGFDDAAVLDGGLDKWKAEGRPLEAGAAKGYPPATFTAAP
                     RPGYFTDRERVRASLGTSNTVILNALGPQFHRGLEPSRYGRPGRIPGSVNVPAASLVG
                     ADKTFVPLADAAAKFAAQGIAKDKDVICYCGGGISATVDLFLLYQLGFDRLTLYDGSM
                     GEWAREPSLPIETD"
     gene            complement(1893..4406)
                     /locus_tag="VFR00_10315"
     CDS             complement(1893..4406)
                     /locus_tag="VFR00_10315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011996086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     ATP-binding protein/permease"
                     /protein_id="HEU0159700.1"
                     /translation="MPNARDLLIAAVFLVLLPFALTSLGLGITSATEVVVFAIACSAL
                     NILVGYTGLTSFGHGAWFGLAAYAAGLAQLKLFPGSILFPVVAGMAVVAVVALAFGYV
                     ILRRRGVYFSLLTLALAAMLYTIAFRWTDVTGGENGLGGITRPVLVGLPLDNAVAFYV
                     LVATVGFAVVCLLWRFHRSPVGHVLVAIRENETRARFIGYQTNRYKLLAFVTSATLTG
                     LAGTLLLFNNRMTSADPISVAFSGELLAMVIIGGMRSFLGPALGALFFVIFRDYLSSI
                     TENWLLYFGLLFVAFIVFSPTGLVGLGERLLAPFATKPVEEAAMAGRQAGSVELPAFL
                     RPRTHLPGAILTVAGLSKSFGGIKAVQGASFSVKDRSLHALIGPNGAGKTTAFNLISG
                     MYTPDAGTVMLEERSVGGLTPGAVTQAGIGRSFQITNLFPALTVEENVRLAVQSRDPK
                     RFSFWSTALSLAEVNSQTAAMIRTMGLAGIERVDAGSLSYGGQRLLDMALALATKPRV
                     LLLDEPLAGLAAAERERIGRLIKQISADIPVLLVEHDIDRVFQLADAVTVMNDGQVLV
                     DGGVEEARSSSKVQEVYIGSGSHALAARVRASAVQPQPLLTLDGVNTFYGKSHILHDV
                     GFTVREKEIVALLGRNGAGKSTLLKTVVGIAPPMTGSIQLAGEELARRPAAEITRRGI
                     SYVPQGRGLFAGMSVAENMELGRLRRRNQAGTYWDEDKIFAFFPRIKERWRSAADYLS
                     GGEQQMVAVARALSGDTRVLLLDEPFEGLAPAVIEELFEAFDKLRQEISIVIVDHHLD
                     LALALSDSTVALERGQIIHVGPSKALSDDLDLRRKVLWL"
     gene            complement(4410..5288)
                     /locus_tag="VFR00_10320"
     CDS             complement(4410..5288)
                     /locus_tag="VFR00_10320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020091907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEU0159701.1"
                     /translation="MQSVLFLQHVLNGLLAGTYYLLIALGLSLIFSLGGIVNLAHGAF
                     YAIGAYLTVLFSPTIGFGGSIVVSPVCVGLLGVIIERTLFRRFYKADPSLSLILTFGL
                     AMVAEQILRMVFGAAPLSFSIPSELRGQLFVAGFIYPYYRILVLLIAAACVAGLWFLL
                     QRTAFGRVVRAGVQSPDMVGALGISLEPYMTAVAFLGVGLAGLAGVMLAPIFSIHPAM
                     GNEIITAAFVVVVIGGLGSFWGVVVAAMIVGLVKGLVVAFGYPQFSEAAMYLLMLLVL
                     LLRPRGLFGERIQRFE"
     gene            complement(5351..6592)
                     /locus_tag="VFR00_10325"
     CDS             complement(5351..6592)
                     /locus_tag="VFR00_10325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007562084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0159702.1"
                     /translation="MTTKTSLSRRRLLQGAGAATLGAFPAISYGQAEGIKIGHLTPRT
                     GFLGTLGEFAVQAVDLATEEINASGGVMGRRLELIKEDSVNPQTASTKAERMVERDKV
                     ACIVGEISSASGLTISQVADRTKTLFINTGCNSDELRGKGCKKFMFHIESANSMYVKT
                     VGRALLANNLVKGKKWFSLTADYAFGHDLLRVAKRFMESNGGQFASDKLVPTDATDFS
                     ALLLEIRSAKPDLVVSNLAGLQITNFLKQYSEFGLDFPVAGFGFDTAVAWGAGKGNFF
                     GTWPLVWHHLIDTPGSKAFVAAFSKKYNKPPENQAWGDYMALKILAQSMNEVKSSEAS
                     KLIEHWEKGAKFDLLKTRPGYFRAWDHQLMHEMYAVEALKAAELKNQWDIYRPSPPVP
                     GANEDLEAIAPTKEENACTFA"
BASE COUNT         1365 a         2234 c         2260 g         1128 t
ORIGIN      
        1 gggcgattgc cgaggccatg gtccgcaatg cccggcccgg ctccatcatt gtttcccacg
       61 ccaacggccg cggctaccac acggccgagg cgctgccgct ggccattccg ctgctcaggg
      121 ccaaaggctt ctcctttgtc acggtgtcgg agctgctggc ggcgggcaag gcggtcatgg
      181 ccgagacctg ctacgatgca catcccggcg acacggacag atacgatttc ttgcgggggc
      241 ataccccaac cgcatctccc tcctgggaac ccaccaccca tgtccgagcc ggcccctccc
      301 ttccgcgctg agcgctatgc cctgcgctcc ctgggcgacg gccgcacttt ggcattggcc
      361 ccgttcagcg ctgcggccgc cgaccgacta ggtccagcga cggccgcgat cgggccctgg
      421 gcgcattatg gtttcgacgg cgacaagctt gcggccgggt tcaagcgcac cgaagagggc
      481 attcaccgct ttcaggtcga atgtgacggc gaccttgcgg gtgccctcat cgtggtctgt
      541 ccctggctcg ccggtcccta cctgcaaatg ctcgcaatcc agccggccta tcagaagcat
      601 ggcatcggcg cgcgcatcct tgcttggtac gaagccgagg cgcgcggcgc cttccgcaac
      661 ctttggctct gcgtcagcgg cttcaatgtc gatgcgcaac ggttctatat cgcacacggc
      721 tatacgcagg tggccagcct cgacggcctg atgcgggagg gtgatgtcga gttgctaatg
      781 cgcaagcgcc tgcaaccctg atgtcggcgc gcggcccggt ggtcgagccg cagggcctcg
      841 tcaatccgtc tcgatcggca gcgatggctc gcgtgcccac tcgcccatgg acccatcgta
      901 aagggtgagc ctgtcaaagc ccaattggta gagcaggaag aggtccacgg tcgccgaaat
      961 gcctccgccg caatagcaga tgacgtcctt gtccttggcg atcccttgcg cggcgaattt
     1021 ggcggccgcg tcggcgagtg gcacgaatgt cttatcggcg ccaacgaggc tcgccgccgg
     1081 cacattgacg ctgccgggga tgcggccggg ccggccatag cggctcggct ccagcccccg
     1141 gtggaactgc ggcccgagcg cgttgaggat gacagtgttt gacgtgccga ggctggcgcg
     1201 gacgcgctcg cgatcggtga aatatcccgg ccggggcgcc gccgtgaagg tggccggcgg
     1261 ataacccttg gctgcgcccg cctccagcgg ccggccttcg gccttccatt tgtcgagacc
     1321 gccgtcgaga accgcggcgt cgtcaaatcc caatgacttg agcatccacc aaaagcgggt
     1381 cgcccacatg ctggtgccga ggctgtacaa gacgacctgg gcgccggcgc caacaccatg
     1441 gcggccaaag gccgcctcga gggacgccgg tgcggcgagc ataaagcgca gcggcgacgc
     1501 gttgtccgag aactcgcctt gcaaatcgag aaaatcggca ccgggaatat gcgcctccgc
     1561 gaagctgcgc cgcccaggca cgacgacata aggctgatca ctcccggcgg gagcggcctc
     1621 cagataggtc gtgcaatcgt aaatcctaag gttcgcctgg ccgagccgag cggcaagctg
     1681 ctcggtacgc ataagggctt ggggatcgcg catgtcctac tccacgcctt ggggcgatgc
     1741 agcgccttga ccccgcacca tgccatagcc gttgcgcgtt tgcatcgggg acgctgtgcg
     1801 cctgccattg gcacccatcc accgcgtccg atctcttgca aaacggcagc cccgagcaac
     1861 gccgccgacc ccttgcggcg gatcgtgcag gcttagagcc acagcacctt gcggcgcagg
     1921 tcgaggtcat cggaaagggc cttggagggg ccgacgtgaa tgatctgccc acgctcaagg
     1981 gcaacggtgc tgtcggacag ggcgagcgcg aggtcgaggt gatgatcgac gatcacgatc
     2041 gagatctcct ggcggagctt gtcgaaggcc tcgaacagtt cctcgatgac ggccggcgcc
     2101 aagccctcga acggctcgtc cagcaagagc acgcgcgtgt ccccggaaag ggctcgggcg
     2161 accgcaacca tttgctgctc gccgccggac aggtagtccg ccgccgagcg ccagcgctct
     2221 ttgatgcggg ggaaaaaagc aaagatcttg tcttcatccc agtaggtgcc ggcctggttg
     2281 cggcgccgca gccggccgag ctccatattc tccgcaacgc tcatacccgc aaacagtccg
     2341 cggccctgcg gcacgtagga gataccgcgc cgcgtgattt cggcggcggg gcgacgcgcg
     2401 agctcctcac ctgccaactg gatgctgccc gtcatcggcg gggcgatgcc gacgacggtc
     2461 ttgagcagcg tcgactttcc cgccccgttg cggccgagca aggccacgat ctctttctcg
     2521 cgcacggtaa agccgacatc gtgcaggatg tggctcttgc cataaaaggt attgacgccg
     2581 tcgagggtca gcaacggctg cggctggacc gcggaggcgc gaaccctagc ggcaagcgca
     2641 tggctgcccg acccgatata gacctcctgc actttactgg aggagcgggc ctcttcgacc
     2701 ccgccgtcga ccaagacctg gccgtcgttc atcaccgtga ccgcgtcggc gagctgaaac
     2761 acgcggtcga tgtcgtgttc gaccagcagc acggggatgt cggcagagat ctgcttgatc
     2821 aggcggccga tgcgttcgcg ctcggcggcg gcgaggccgg cgagcggctc gtcaagaagc
     2881 aggacgcgcg gcttggtcgc gagcgccagg gccatgtcga gcaggcgttg gccgccatag
     2941 gatagcgagc cggcgtcgac ccgctcgatg ccggcgaggc ccatggtgcg gatcatggcg
     3001 gcggtctggc tgttgacctc tgccaggctg agggcggtgc tccagaaact gaagcgtttg
     3061 gggtcgcgcg actgcacggc gaggcgcacg ttttcctcga ccgtgagcgc cgggaagaga
     3121 ttggtgatct ggaaggacct gccgataccg gcctgggtca cggccccggg cgtcaatccg
     3181 ccaaccgaac gctcctccag catgaccgtt cccgcgtcgg gcgtatacat gcccgagatc
     3241 aggttgaagg ccgtggtttt gccggcgccg ttcggaccaa taagggcgtg caggctgcga
     3301 tccttgacac tgaaacttgc gccctgcacg gccttgatgc cgccaaagct cttggaaagc
     3361 cctgccaccg tcaaaatcgc cccgggcaga tgggtgcgcg gccgcaagaa tgcgggcaac
     3421 tcgacgctgc cggcctgacg gcccgccatg gcggcctctt ccaccggctt tgtggcaaac
     3481 ggcgccaaca gccgctcccc caagccgacc aagcccgtgg gcgagaacac gatgaaggca
     3541 acgaacaaga ggccgaagta gagcagccaa ttctcggtga tgctcgacag atagtcgcga
     3601 aagattacga agaagagggc gccgagagcg ggtccgagaa aggagcgcat tcccccgata
     3661 atgaccatgg caaggagttc gccggagaag gcgaccgaga tcggatcggc cgacgtcatg
     3721 cggttgttga aaagcaggag cgtgccggcc aacccggtga gcgtcgccga ggtcacgaac
     3781 gccaagagct tgtagcgatt ggtctgatag ccaatgaagc gggcgcgcgt ctcattctcg
     3841 cggatggcga ccagcacgtg tcccaccggc gagcggtgaa agcgccacag caggcacacc
     3901 acggcaaagc cgaccgtggc gaccagcacg taaaaggcaa ccgcgttatc gagcggcagg
     3961 ccaacaagca ccggccgggt gatgccgccg aggccatttt cgccaccggt cacgtcggtc
     4021 cagcgaaagg cgatggtata gagcatcgcc gccaaggcga gcgtcagcag cgagaagtac
     4081 acaccccgcc ggcgcaggat cacgtagcca aaggccaagg cgacgacggc tacgaccgcc
     4141 atgcccgcca ccaccgggaa cagaattgat cccgggaata atttgagctg cgccagcccc
     4201 gcggcgtagg cggccaagcc gaaccaggcg ccgtgaccga atgaggttag gccggtgtag
     4261 cccaccagaa tattgagcgc gctgcaggca atcgcgaaca ccacgacctc ggtggcggag
     4321 gttatgccga ggccaagcga ggtgagcgcg aagggcaaga gcacgaggaa aacggcggca
     4381 atgagaaggt cgcgtgcgtt tggcatgtcc tactcgaacc gctggatgcg ttcgccgaag
     4441 agaccccgcg gccgcaatag caggaccaac aacatcaata agtacatcgc cgcctcggag
     4501 aactgcgggt aaccgaaggc caccaccaat cccttgacca gaccgacgat catggctgcc
     4561 accacgacgc cccagaacga gccaagcccg ccgatgacca ccaccacgaa agcggccgtg
     4621 atgatctcgt ttcccatcgc cggatggata gagaagatcg gcgccagcat gacgccggcg
     4681 agccccgcca ggccaacacc gaggaaggcc accgccgtca tatagggctc gagcgagatg
     4741 ccgagcgcac cgaccatgtc cgggctttgc acgcctgccc gcacgacgcg cccaaaggcg
     4801 gtgcgctgca gcagaaacca aagcccggca acgcaggccg ccgcgatcag gagcacgagt
     4861 atgcggtagt aaggatagat gaagccggcc acgaagagtt ggccgcgcag ttcggatgga
     4921 atggaaaagg acaggggtgc cgcgccgaag accatgcgca agatctgctc ggcgaccatg
     4981 gccagcccga aggtcaggat caaggagagg ctcgggtcgg ccttgtaaaa gcggcgaaac
     5041 agggtgcgct cgatgatcac ccccaatagg ccgacgcaca ccggtgagac cacgatcgat
     5101 ccgccgaagc cgatcgtcgg cgagaacagc acggtcaggt aggcgccgat cgcgtaaaac
     5161 gccccgtgcg caagattgac gatgccgccg agggaaaaga tgagggacag tccgagcgca
     5221 atgagcaggt agtaggtccc tgccaagagg ccgttcagga cgtgctgcaa gaaaagaacg
     5281 gactgcaccg ggctcctcca ctccctggcg gcggggaggc gttccgcctc ccctcacgac
     5341 cctcgccggc tcaagcgaag gtgcaggcgt tctcctcctt ggtcggcgcg atggcctcga
     5401 ggtcctcgtt ggcgcctggc accggcgggc tgggccggta aatgtcccat tggtttttca
     5461 actcggcggc cttcaacgcc tccaccgcgt acatctcatg catcagctga tgatcccaag
     5521 ctctgaagta gccggggcgc gtcttgagca ggtcgaactt ggcacctttt tcccagtgct
     5581 cgatcagctt ggaggcctcg ctcgatttta cctcgttcat ggactgggcc aaaattttca
     5641 aggccatgta gtcgccccag gcttggttct cggggggctt gttgtatttc ttgctgaagg
     5701 ccgccacgaa cgccttggag cctggcgtat cgatcaggtg atgccaaacc agcggccagg
     5761 tcccgaagaa gttccccttg ccggctcccc aggcgaccgc cgtgtcgaag ccgaaaccgg
     5821 ccaccggaaa gtcgagcccg aactccgagt actgcttgag aaaattggtg atctggaggc
     5881 cagcgaggtt cgacaccacg aggtccggtt tggccgagcg gatctcgagc agtagggccg
     5941 agaagtcggt ggcgtcggtg ggcaccaact tgtcggacgc gaattgcccc ccgttgcttt
     6001 ccataaaacg cttggccaca cgcaagagat catggccgaa ggcgtagtcg gcagtcagcg
     6061 agaaccactt cttgcccttg acgagattgt tggcgaggag ggcccgccca accgtcttca
     6121 catacatcga attggccgac tcgatgtgga acatgaattt cttgcagccc ttgcccctca
     6181 gttcgtcgga attgcacccg gtattgatga acaaggtctt ggtgcggtcg gcgacctgcg
     6241 agatggtcag tcccgaggcg gacgagattt ccccgacaat gcaggcaacc ttgtcgcgct
     6301 ccaccatgcg ctcggccttg gtggaggccg tctgcggatt gaccgagtct tccttgatga
     6361 gctctagtct gcgacccatc acaccgccac tcgcattgat ctcctcggtg gcaagatcta
     6421 ccgcttgcac ggcgaactca ccgagagtgc cgagaaaacc cgtgcgcggc gtcaggtggc
     6481 caatcttgat gccttcggcc tggccatagg aaatggctgg gaaggcgccg agggtcgccg
     6541 cgccggcgcc ctgtagcaga cgacggcgtg atagtgacgt cttcgtggtc atgagcgttc
     6601 ctctgtttac ccgctggccc agccgttgtg ccgatcttat gcaaagacgg tacggcaatt
     6661 cgggccgaca ggtccaggtt tgcaaagggc atcggcaggg ctcgattggc atggcagggg
     6721 cgcagagcga ggcttggcgc agcggcgacg gcccacttgg agccccggcc gcgtgcctcg
     6781 ccgagagcca ccggcccttc gctgagcgcg catggcttgg ccctcgttaa ggcacccctc
     6841 caatggggcg ccgggcgtca tggcgccccc agccgcgatg ccctaaggtt gtcgggaacc
     6901 tttgctggag ccgcgcatcc aaaggggcaa caaaccaggg aagatgccat ggcaaggctg
     6961 gagcgtcgaa acgacagcgc actcgat
//
LOCUS       DASTMT010000444         2997 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_6363719, whole genome shotgun sequence.
ACCESSION   DASTMT010000444 DASTMT010000000
VERSION     DASTMT010000444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2997)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2997)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2997
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6363719"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..656)
                     /locus_tag="VFR00_10330"
     CDS             complement(<1..656)
                     /locus_tag="VFR00_10330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018453240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HEU0159703.1"
                     /translation="MTTLLLAEHDNKTLAPTVAKAMTAARALGEDVDVLVAGADCKAV
                     AEAAAKLEGAHKVLHADAPQFAHQLAEEMASLIVGLMGSYKAVVAPSTASGKNIMPRV
                     AALLDVMQISDIIKVVGPNSFERPIYAGNAIQTVESADAKKVITVRTTAFAAAPATGS
                     AHLESLTAPAPAGLASFERAEIQKSDRPELTAAKIIISGGRGLQSGENFKTYIEPVAD
                     "
     gene            complement(653..1408)
                     /locus_tag="VFR00_10335"
     CDS             complement(653..1408)
                     /locus_tag="VFR00_10335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020091850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HEU0159704.1"
                     /translation="MKALVAVKRVVDFNVKIRVKADGSGVELSNVKMSMNPFDEIAVE
                     EVVRLKEKGVLKEIVAVSIGPSKSQETIRTALSMGADRGLLIETPDGQTIEPLAVAKL
                     LKAVVETEKPQFIILGKQAIDDDCNQTGQMLAALLGWPQATFASKVSLEADAAFVTRE
                     VDGGLETLKMKLPAVITTDLRLNEPRYPSLPNIMKAKKKPLEIRKAAEFGVDIAPRLK
                     VLKTVEPPGRKAGVKVANAAELVAKLKEAGALA"
     gene            complement(1667..2374)
                     /locus_tag="VFR00_10340"
     CDS             complement(1667..2374)
                     /locus_tag="VFR00_10340"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019964348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEU0159705.1"
                     /translation="MVFIPLSDDNPLRSISFQWVNVGLIVANVVAFFLQNTEGAQASI
                     LSFALIPSELFQAHLVGGAVPSPQQPVPIPEGLTLLTYQFMHGDIFHLLSNMLFLWVF
                     GDNIEDALGHVKYLIFYLLCGVAGGLLHAVMLPSSPLPLIGASGAVAGVIGAYLLLHP
                     RVLVWVLAFRIIPLHIRAAWVLGIWVATQVIMVLVSPVDQVAWWAHIGGLAAGAILVV
                     FMRRPGVPLFDRDLRPV"
     gene            complement(2429..>2997)
                     /locus_tag="VFR00_10345"
     CDS             complement(2429..>2997)
                     /locus_tag="VFR00_10345"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007615316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HEU0159706.1"
                     /translation="NKIYTRTGDAGFTALGIGERVPKHSPRIAAYGTVDEANAFIGVA
                     RLQLGGEPKLDAMLARVQNDLFDLGADLTAPEREGKPQRERLRISEAQVRRLESEIDE
                     LNAELAPLTSFVLPGGSAGAAALHVARTVCRRAERAMVALASTPHEHVSVPGLQYINR
                     LSDLLFVASRYANQRGRADVLWVPGQNR"
BASE COUNT          533 a          944 c          985 g          535 t
ORIGIN      
        1 ttgtcggcga ccggctcgat gtaggtcttg aaattttccc ctgactgcag gccgcgccca
       61 ccggagatga tgatcttggc cgctgtcaac tcgggccgat cggatttttg aatttcggcc
      121 cgttcgaagc tggcaagccc cgccggggcg ggtgcggtca ggctctccaa atgtgctgag
      181 cccgtcgctg gcgcggccgc gaacgcggtg gtgcgcaccg tgatgacctt tttggcatcg
      241 gcgctttcca ccgtctggat ggcattgccg gcgtagatcg ggcgctcgaa gctgttgggg
      301 cctacgacct tgatgatgtc ggagatctgc atgacgtcga gcagcgccgc cacgcgcggc
      361 ataatattct tgcccgaggc agtcgacggc gccacgacgg ccttgtagct gcccatcagc
      421 ccaacgatga ggctcgccat ctcttccgcc agctggtgcg cgaattgagg ggcatccgcg
      481 tgaaggacct tgtgggcgcc ctcaagcttg gcggcagcct cggccaccgc cttacaatcg
      541 gcacctgcca ccaggacatc gacgtcctcg cccaacgccc gcgcggcggt catggccttg
      601 gccacggtcg gcgccaacgt cttgttgtcg tgctcggcca gaagaagggt ggtcatgcga
      661 gcgcgccggc ctctttcagt ttggcgacga gttcggcggc gttcgccacc ttgacgccgg
      721 ccttacggcc tgggggttca accgtcttga ggaccttgag gcggggggcg atgtcgacgc
      781 caaactcggc cgccttgcgg atctcgagcg gcttcttctt ggccttcatg atgttgggga
      841 gcgagggata gcgcggctcg ttgaggcgca ggtcggtggt aatgacggcg ggcagcttca
      901 ttttcagggt ctccagcccg ccgtcgacct cccgcgtcac gaacgccgca tcagcctcga
      961 gtgaaacctt tgaggcaaac gtcgcctgcg gccaacccaa cagcgccgcc agcatctgtc
     1021 ctgtttgatt gcagtcgtcg tcgatcgcct gcttgccgag gatgatgaac tggggcttct
     1081 ccgtctccac caccgccttc aacagtttgg cgacggccaa aggctcgatg gtctgcccgt
     1141 ccggcgtttc gatcagcagg ccgcgatcgg cacccatcga cagtgcggtg cgtatcgttt
     1201 cctgcgattt gctggggccg atggaaacgg cgacaatctc tttgagcacc cccttttcct
     1261 tgagccgcac cacctcttcg acggcaattt cgtcgaaggg gttcatcgac atcttgacgt
     1321 tggagagctc aactccggat ccgtcggcct tgacccggat cttcacgttg aaatcgacga
     1381 cgcgcttgac agcgacgagg gctttcattc cacgaggtgc tccatgtcag catgaggtgt
     1441 cccggcccct catgccgcac tgcggtcgct tgctcctgcc gcaaacgggc aaatttgcct
     1501 gatttatggc gacgcgaatt ggcgcttcgc agatgcgaaa tggaaaccta gggcgcccga
     1561 gagtgcgtgt caattgggca gcagccgaga cactttggac aaaggcaagg caacagggcc
     1621 tgtggcgcaa gcgccgcgct tttggcacct ccaccccggt attgcctcag accgggcgca
     1681 ggtcacggtc gaacagcggg accccgggcc ggcgcatgaa caccaccaag atggcgcccg
     1741 ccgccagccc cccgatatgg gcccaccagg cgacctggtc gacggggctg acgaggacca
     1801 tgatgacctg ggttgccacc cagatcccga gcacccaagc ggcgcggatg tggaggggaa
     1861 tgatgcgaaa ggccagcacc cagacgagca cgcgcggatg cagcagcaaa taggcgccga
     1921 tcacgccggc cacggcgccg ctggccccga tcaggggcag gggtgaactc ggcagcatga
     1981 cggcgtgcag aaggccgccg gccacgccgc acaagaggta gaaaatcaga tatttcacgt
     2041 gcccgagggc gtcttcgatg ttgtcgccga acacccagag gaacagcata ttcgagagca
     2101 ggtgaaagat gtcgccgtgc atgaattgat aggtgaggag cgtcagtcct tccgggatcg
     2161 gcaccggctg ctgcggggag ggcacggctc cgccgacgag gtgggcctga aacagctcgg
     2221 aggggatcag cgcgaaactc aagatgctcg cctgcgcacc ttccgtgttc tgcagaaaga
     2281 aggccaccac gttagcgaca atcaggccga cgttgaccca ctgaaagctg atcgagcgca
     2341 ggggattgtc gtcggagagg gggatgaaga ccacgggtgc ttagtccttt ctgaacgacg
     2401 gggaggtgcg ccgcgccacc cgtaaacctc agcggttctg gccgggcacc caaagcacgt
     2461 cggcgcggcc gcgctgattg gcgtagcggc tggcgacgaa caacagatcc gacaagcgat
     2521 tgatgtattg cagcccgggg acgctgacgt gctcgtgcgg cgtgctcgcc agcgccacca
     2581 tggcccgctc ggcgcggcgg cagacggtgc gcgccacgtg caaggccgcg gcgccggcgg
     2641 agccgccggg caacacgaag gaggtgagcg gcgccaactc ggcgttgagc tcgtcgatct
     2701 cgctttccag acggcgcacc tgcgcctcgc tgatgcgcag ccgttcgcgc tgaggcttgc
     2761 cctcgcgctc cggcgcggtg aggtcggcgc cgagatcgaa caagtcgttc tgcacgcgcg
     2821 ccagcatggc atcgagcttc ggctcgccgc cgagctggag ccgcgccacg ccgataaaag
     2881 cgttggcttc gtcgaccgtg ccataggcgg cgatgcgcgg cgagtgcttg ggcacgcgct
     2941 caccaatgcc gagcgcggtg aaacctgcat ccccggttcg cgtgtagatc ttgttga
//
LOCUS       DASTMT010000445         2537 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_1238147, whole genome shotgun sequence.
ACCESSION   DASTMT010000445 DASTMT010000000
VERSION     DASTMT010000445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2537
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1238147"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(132..1406)
                     /locus_tag="VFR00_10350"
     CDS             complement(132..1406)
                     /locus_tag="VFR00_10350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008968751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEU0159707.1"
                     /translation="MRRVVVVVLDGMRRDQILQANTPGLVRFAGRAQSFVEHRSMFPS
                     ATRVVSASFATGCLPARHELQGNSLALLEDGRLVQHDAGHPDFLQHKRRVTGRALAVP
                     TLAERLAPIGGMILFSNVSPGAAYAHDPDGFGYVYHRAGSYAPGRKRLEGTQALTIAQ
                     DAAGERLMTERFMREVLIERSPPLAVLWLGEPDATQHLQPLGSPAQLAAVAQADGNAA
                     RVIAAVDALRARGDDVLLIVCSDHGHQSVSRVVDIEAELIAAGLKDGRESTDVVTPTS
                     GTSTLIYVAANCADRIGAIGAFLLSRDWVDRVVGPDELGAVGQAADHGLAFAISLKSD
                     DEPNAFGVRGRSVEARPAAGKADHLGCGQHGGLARYEQMPFLMIEGPGFGAGSTARTP
                     TSPIDLAPTILAHLDQPQTGMDGRALQAPGGR"
     gene            1578..2447
                     /locus_tag="VFR00_10355"
     CDS             1578..2447
                     /locus_tag="VFR00_10355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEU0159708.1"
                     /translation="MPTRPSPSIAFVIGLAIVAEGLLTLMDAMIKSLAPRYSAIEIAF
                     LRYAFGMIGAVVYAFATRPGWPTGEATIYNGLRAVLIVFTATTFFFALGKLSLADAIA
                     MSFISPALTAVLGVLILSERLDWRIVIGLVGGFLGMLLIVGAKLGGGGTDGGVLLGAI
                     AVFLSAIGYSLNVIMLRHRATRDPLAQIILFQNVGPALILALPALWLWRTPPLADLMQ
                     FAVLGTIGVIAHTMLAHAFARVEAARLAPVGYVTLVWGVLFGFLFFADVPGLATLMGS
                     ALIVVGTLFAQRR"
BASE COUNT          425 a          813 c          810 g          489 t
ORIGIN      
        1 agggacgaaa ttgatcggac gatggggcag atgggcgcgc ccgatatccg cagccttggt
       61 ccgcagttcc taatgtggag cgaggaagac gatttgcggc gcaacgtgcg cagctgaggg
      121 cgatgctctt gctagcgccc gcccggtgcc tgcagggccc gtccgtccat cccagtttgg
      181 ggctggtcca ggtgggcaag tatcgtcggt gctagatcaa tcggtgaggt tggtgtcctg
      241 gccgtgcttc ctgccccgaa acccgggcct tcgatcatca ggaacggcat ctgctcgtag
      301 cgcgccaagc caccatgctg cccacagccc aggtggtccg ccttaccggc ggcgggacgc
      361 gcctcgacgc tgcggccgcg gacgccgaaa gcattcggct cgtcgtccga tttgagtgaa
      421 atggcaaagg ccaggccatg atcggcggcc tgccccaccg cgcccaactc atcggggccg
      481 accacgcgat cgacccaatc acggctcagc aagaaagcgc cgatggcgcc aatacgatcg
      541 gcgcaattgg cagcaacata aatcagcgtt gacgtgccgc tcgtgggcgt gacgacgtcg
      601 gtcgactcgc gcccgtcctt caggccggcg gcgatcagct ccgcctcgat gtcgacgacg
      661 cgcgagacgc tctggtggcc gtgatccgaa caaacgatga gcagaacatc gtcgcctctg
      721 gcgcgcaggg catcgaccgc tgcaatcacg cgcgccgcgt tgccatcggc ttgggcgacg
      781 gcggccaact gcgcgggcga gccgaggggc tgcaagtgtt gggttgcatc gggctcgccc
      841 agccacagaa cggcaagtgg cggtgaccgc tcgatcagca cttcgcgcat gaagcgctcc
      901 gtcatcagcc gctcgcctgc cgcgtcctgg gcaatcgtca aggcttgcgt gccctcgagg
      961 cgcttgcgac caggcgcgta agaacctgcc cgatggtaaa cataaccgaa gccatcgggg
     1021 tcatgcgcgt aggcagcgcc aggcgagaca ttggaaaaaa ggatcatacc gccaatgggt
     1081 gccaagcgct cggccagcgt tggtaccgcc aaggctcgac cggtcacgcg ccgtttgtgc
     1141 tgcaaaaaat cggggtggcc ggcatcatgc tgcacgaggc ggccgtcttc cagtaatgcc
     1201 agcgaattgc cttgcagctc atgtcgagcg ggcaggcagc cggtggcgaa ggaggccgaa
     1261 accacgcgcg tcgccgatgg aaacattgag cgatgttcga caaacgactg cgcccgcccg
     1321 gcgaaacgga cgaggcccgg cgtattggcc tggaggatct ggtcccggcg catgccatcg
     1381 agcacgacca caacgacgcg acgcacgaag cccccttttc acgatagttg ccactaatcc
     1441 cgagctggcg cggatatcga ttgtcacaga ctagatcagg cgcaaagccg cggccagtgg
     1501 gtaagcgcgc caatgccttc gcccctgcga gattggtcat tggtagcgcg ggcagaagcc
     1561 gataggttgc gccctccatg cccacccggc cgtcacccag catcgccttc gtcattggcc
     1621 tcgctatcgt cgcggagggc ctgctgacgc tgatggatgc aatgatcaag tcgctggcgc
     1681 cgcgctatag tgccatcgag attgctttcc tgcgctacgc tttcggcatg atcggcgccg
     1741 tcgtctacgc attcgcgaca cggccgggtt ggccgaccgg ggaggcgaca atctataacg
     1801 gcctgcgcgc cgtgctgatc gtcttcaccg ccactacctt ctttttcgcg ctcggcaagc
     1861 tgtcgctcgc cgatgccatt gccatgagct tcatttctcc cgcgcttact gctgtgcttg
     1921 gcgtgttgat attgagcgag agactcgact ggcgcattgt cattggcctg gttggcggct
     1981 ttctcggcat gctgctgatc gtcggcgcta aactcggcgg cggtggcacc gatggcggcg
     2041 tgctgctggg cgcgatcgcc gtatttctct cggcaatagg ctactcgttg aatgttatca
     2101 tgctccggca ccgggcaacg cgcgatcctt tggcccaaat catcctgttc cagaatgttg
     2161 gaccggcgct gattcttgca ctgccggcgc tatggctgtg gcgcacaccg cccctcgccg
     2221 acctcatgca gtttgccgta ctcggcacga tcggcgtgat tgcccatacc atgttggccc
     2281 acgcctttgc gcgcgtcgag gccgcgcgcc tcgccccggt cggctacgtt acgcttgtct
     2341 ggggcgtgct gttcgggttt ctgtttttcg ccgacgtgcc cggcctggcg acgttgatgg
     2401 gctcggcctt gatcgtggtt ggcaccttgt tcgcgcaacg gcgctagcgc tgccgacgcg
     2461 gcgcctctgc tcgacgggtt aggggatgcc cgctttcagg ctcatggcgc gtctatcttg
     2521 ccgtcatgct gcgcgct
//
LOCUS       DASTMT010000446         2680 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_583535, whole genome shotgun sequence.
ACCESSION   DASTMT010000446 DASTMT010000000
VERSION     DASTMT010000446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2680)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2680
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_583535"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..998)
                     /locus_tag="VFR00_10360"
     CDS             complement(<1..998)
                     /locus_tag="VFR00_10360"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEU0159709.1"
                     /translation="MSRRPRASGYFRTDYGQDLRLLPTRLQRLAAVGVLALLLALPLI
                     ASPFLIDLAAQVCLAVIGALALMLLTGYAGQVSLGHAGLLAAGAFTVGILFKEVGAPF
                     FVTLPAAASVGALLGVVFGLPSLRVRGLYLAVSTLALNFLVAYLGGEYETRRGFSTGI
                     LVDPPVLAGKALTDPRAWYLVLLAAAVASLLLCANLARARSGRAWAAIRTNETVAQTL
                     GIGVASYKLLAFVASSALTAVAGALFAYYRGFVSVEAFSLFLSIQYIAMIIIGGMGSL
                     AGAILGALFVTLFPYAIEAGLSALIGRTAYAGLQFAVNAAAFGAVMILFLLLEPQG"
     gene            complement(991..1863)
                     /locus_tag="VFR00_10365"
     CDS             complement(991..1863)
                     /locus_tag="VFR00_10365"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEU0159710.1"
                     /translation="MQLAVNIIVLTAIYALIACGYVLIYRVSRVLNLAHGELLMLSAY
                     LLLWTASLFGGHPVAAITAALGLSAAVGAMVYLFLLRKLTGEMALAAVLTTVALGVLV
                     RGLTVIVWSAQQQYPAVPLGLANFALALPGGARISLWAALLVATAFVVYASLFAFLRL
                     GRWGMRMRAAGQSPLLAAQRGINLHAIYALAWSLSTLTGALAGILMALDSGLGQTMAI
                     VGLKAFPAALVGGLDSLAGALLGALIVASAEVTLIHYVDPLLSEVIPFLVLIVVLMLR
                     PWGLLGTREELDRV"
     gene            complement(1886..>2680)
                     /locus_tag="VFR00_10370"
     CDS             complement(1886..>2680)
                     /locus_tag="VFR00_10370"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0159711.1"
                     /translation="SSVCPKEVYPPADPLQFCTTAFASTYDSRATLAFIKDTAKEPVK
                     IGFSAMAIPLSRAEMDFAEQQAPALGMTVVDKEVIPPPTPDYTPFATKLKDAGANWVF
                     SWAPWVTQVRTLEALRRLDWKGDYIAWAHIEAEGELARLKDGSFYVVGANALFQEQLP
                     IHQEIAAAAKSAKLTYPVEQLSEGWIAGMVVEAALKATGWPATPEKLAAAMSTLKVDT
                     KGLRGGPIEWTKDNHFRTRQYYRVYRWDGGKGAIERVKDWTAFDVK"
BASE COUNT          535 a          821 c          961 g          363 t
ORIGIN      
        1 aagccctgcg gttcgagcag caagaacagg atcatgacgg cgccgaaggc cgcggcgttg
       61 acggcaaact gcaggccggc gtaggccgtg cggccgatca gagcgctgag gccggcctcg
      121 atggcgtagg gaaagagcgt gacgaacagg gcgccgagga tggcgccggc cagcgagccc
      181 atgccgccga tgatgatcat ggcgatgtac tggatggata agaacagcga aaaggcctca
      241 accgagacga agccgcggta gtaggcaaag agcgcgccgg cgacggccgt cagcgccgag
      301 gaggcgacga aggcgaggag tttgtagctg gcaacgccga tgccgagcgt ttgcgccacc
      361 gtttcgttgg tgcggatggc ggcccaggcg cggcccgagc gggcgcgcgc caggttggcg
      421 cacaggagaa ggctggcgac ggcggccgcc agcaaaacga ggtaccaggc tctcgggtcg
      481 gtcagggcct tgccggcgag caccggtgga tcgacgagaa tgcccgtcga aaaaccacgc
      541 cgcgtttcgt attcgccgcc caggtaggcg accagaaaat tgagtgccag cgtactgacc
      601 gccaggtaga gaccgcgcac gcgcagcgag ggcagcccga agacgacgcc caatagggca
      661 ccgacgctgg cggcggccgg cagcgtcacg aagaacgggg cacccacttc cttgaagaga
      721 atgccgacgg taaaggcgcc ggccgccagc aggccggcat ggccgagcga gacctggccc
      781 gcgtagcccg tcagcagcat cagggccaag gcgccgatca cggccaggca gacctgggct
      841 gccagatcga tcaggaacgg ggaggcgatg agcggcaggg ccagcagcag agccaacacc
      901 ccgacggcgg ccaagcgctg aagccgcgtc ggcaggaggc gcaaatcctg cccgtagtcg
      961 gtgcggaaat agccgctggc gcgcggtcgc ctagacacga tcgagctctt cgcgggtgcc
     1021 gagcaagccc cagggtcgca gcatcaggac gacgatcagc accaagaacg ggatgacctc
     1081 ggacaacagc gggtcgacgt agtgaataag ggtcacctcg gcgctggcaa cgatcaaggc
     1141 gccgagcagc gcgccggcga ggctatcgag gccgccgacc agcgccgccg gaaaggcctt
     1201 gaggccgacg atggccatgg tctggccaag gccggaatcg agcgccatca agatgccggc
     1261 gagcgcgcct gtcagggtgg agagcgacca agccagcgcg tagatggcgt gaagattgat
     1321 gccgcgctga gccgccagca gtgggctctg cccggccgcg cgcatgcgca tgccccagcg
     1381 tcccaggcgc aaaaaggcaa acaggctggc gtagacgaca aacgccgtcg ccaccaacag
     1441 cgccgcccag agcgagatgc gcgcgccgcc cggcagcgcc aaggcaaagt tggcgaggcc
     1501 gagcggcact gccggatact gctgttgggc ggaccagacg atgacggtca ggccgcgcac
     1561 gagaacgccg agggcaacgg tggtcagcac cgcggccagc gccatttcgc cggtcagctt
     1621 gcgcagcagg aacagataga ccatggctcc gaccgccgcg ctcaggccga gcgcggcggt
     1681 gatcgcggcc acgggatggc cgccgaacag cgaggccgtc catagcagca ggtaggccga
     1741 cagcatcaac agttcaccgt gcgccaagtt gaggacccgg ctgacgcgat agatgaggac
     1801 atagccgcag gcgatcagcg cataaatcgc ggtcaagacg atgatattga cggcgagttg
     1861 cacgggcggt gagagccttg ctgcgttatt tgacgtcgaa ggcggtccag tccttgaccc
     1921 gttcgatggc gcccttgccg ccgtcccagc ggtagacgcg gtagtattga cgggtgcgga
     1981 agtggttgtc cttggtccat tcgatcggtc cgccgcgcag gcccttggtg tcgaccttaa
     2041 gcgtgctcat ggcggcagcc agcttctcgg gtgtcgccgg ccagcccgta gctttgagcg
     2101 cggcttccac caccatgccg gcaatccagc cttcgctcag ttgctcgacg ggataggtga
     2161 gctttgccga cttggcggca gcggcgatct cctggtggat cggcaactgc tcctggaaca
     2221 gggcgttggc gccgacgacg taaaaggagc cgtctttgag ccgcgccagc tcgccttccg
     2281 cctcgatatg ggcccaggcg atgtaatcac ctttccagtc gaggcggcgc aaggcctcga
     2341 gcgtgcgcac ctgggtgacc cagggcgccc aggagaacac ccagttcgcc ccggcatcct
     2401 tgagtttggt ggcgaagggc gtgtagtcgg gcgtgggcgg tgggatcacc tccttgtcga
     2461 ccacggtcat gccgagcgcg ggcgcctgct gctcggcaaa gtccatctcg gcgcgcgaga
     2521 ggggaatggc catggcagaa aagccgattt tgaccggctc cttggcggtg tccttgataa
     2581 aggcgagggt tgcccggctg tcataggtcg aggcgaaggc ggtcgtgcag aattgcagag
     2641 gatcggcggg aggatagacc tccttggggc acaccgagct
//
LOCUS       DASTMT010000447         8347 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_250216, whole genome shotgun sequence.
ACCESSION   DASTMT010000447 DASTMT010000000
VERSION     DASTMT010000447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 8347)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8347)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8347
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_250216"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..406
                     /locus_tag="VFR00_10375"
     CDS             <1..406
                     /locus_tag="VFR00_10375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009340762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEU0159712.1"
                     /translation="IVAAFNIISGLIMLVKDKGRDIGILRTMGASKGAIMRVFLITGA
                     SIGVVGTLAGALLGIVFCWKIDEIRQFVAWLTNTRLFDPEIYYLTRLPADINPQTTAV
                     IVGMALVLSVLATLYPSWRASRLDPVEALRYE"
     gene            412..1125
                     /locus_tag="VFR00_10380"
     CDS             412..1125
                     /locus_tag="VFR00_10380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010910090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0159713.1"
                     /translation="MQDATPRPVLELRQVERTFKQGDREIPVLTGANAELYAGQAIAL
                     VGPSGAGKSTLLHIAGLLETPDGGRVLVNGVDCARLSDGERTRVRRREMGFIYQFHQL
                     LPEFTALENVLMPQLIAGTARRRGEARAKELLTMLGLGHRLEHRPAQLSGGEQQRTAI
                     ARALANAPKLLLADEPTGNLDPQTSAHVFHELIELIRHSGVAALIATHNLALAKRMHR
                     VLVLDSGRLQEVNPASVPG"
     gene            1344..2528
                     /locus_tag="VFR00_10385"
     CDS             1344..2528
                     /locus_tag="VFR00_10385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013419905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III PLP-dependent enzyme"
                     /protein_id="HEU0159714.1"
                     /translation="MERYATAAALIGAHKPDRPVIGLRPHAAGRAARWFLQNFPGDVA
                     YAYKANSSVFLLGALYGAGVRHFDVASLTEIEDAQTIPEAQLHFMHPVKSRSAIRRAF
                     AEFGVRSFALDSEEELKKIAEETGGCRDLTLWVRVAVSAKNSKIPLERKYGVAGAKAV
                     RLLGKARQAAKTLGVTFHVGSQTATPDAFVTALSEVGRLIVKAGVVLDRLDVGGGFPS
                     IYSDDAPAPLAAFVRAISDCVDRLPVGEHCRLMCEPGRALVAEAESLIVRVDARRGNE
                     LFINDGSYGTLFDAAHMGFAFPVRLVGHELEMGETLVPFAFWGPTCDTIDFMPGPFLL
                     PSRVKEGDYIEIGNTGAYGRALGGRFNGYGAYDEAILLDEPLITMYGESAVESAAQQV
                     QA"
     gene            2525..3436
                     /gene="speB"
                     /locus_tag="VFR00_10390"
     CDS             2525..3436
                     /gene="speB"
                     /locus_tag="VFR00_10390"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HEU0159715.1"
                     /translation="MSAKLEYLSAAESFLDPVRADADQPASARAVIVPFGLEASVSYG
                     TGTAAGPAAILKASQQLELYDDELGREAYLDYGVAALREPAIAMPIAAALEQVQGLVE
                     AVVAAGKFPLVLGGEHSLTAGAIRPLLARHADLVVLQFDAHADLRDGYLGEPYSHAAA
                     MRRVLDHAGVSLIQVGLRSVAAAEAAYMAQHRDRISVYWAKDQAAWDIDGLVRQLGGR
                     PLYVSFDIDALDAAVMPATGTPTPGGLGYWQALALLRRACAAGRVVGADLVEFAPIAS
                     LHAYDYTAAALAYKLLNYALCGAERPG"
     gene            complement(3433..4686)
                     /locus_tag="VFR00_10395"
     CDS             complement(3433..4686)
                     /locus_tag="VFR00_10395"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0159716.1"
                     /translation="MADWHLWLPPALSKPRFRLYVAGHAVSVIGGWIQQVAIAWLVFR
                     LTNSAFLLGVAGFLLNIFYLLLGPVAGLAADRLPRLKSLIIIDLLLASLSVLLAGMAA
                     AGVETVTAYLAIAALIGAANAFEMPVRQTLIRIIVEERALVTSALGMSAMVFNVGRML
                     GPAIAGIVLAYLSEAWCFALNAMSYAAIIAALLAMRLPPEPRGATAAAGAQQTFGQSL
                     QVLFAFPAVRYLLPVCVALGLLATPYVPLMPSIVSHFFDGQSSTLGLLMSSAGIGALA
                     SATYLSLQPGYGRQIRLMTLAPLAVGAALTLFAWSRTLPISLVLLAILGGAALLGVNA
                     TNAMLQQSVPDAWRGRVIGLYSMSFAGTAPIGGLIAGYCAERIGLTTTLTINGVLILV
                     AGFASRWRLHNHPEALRGLMRSLTR"
     gene            complement(4791..4865)
                     /locus_tag="VFR00_10400"
     tRNA            complement(4791..4865)
                     /locus_tag="VFR00_10400"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4831..4833),aa:Val,seq:cac)
     gene            complement(4956..5966)
                     /locus_tag="VFR00_10405"
     CDS             complement(4956..5966)
                     /locus_tag="VFR00_10405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015928009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEU0159717.1"
                     /translation="MRYRPLGVSGLKVSEISLGTMMFGGPAGAAEARRMLDYALEQGV
                     NFVDTANVYSETRSESVLGDAVQGKRNAWIIASKVGNKLGAGPNDSGLSRRHVMDAVE
                     GSLRRLKTDCIDLYYIHRVDADTAWENVVQTFSDLIRSGKIRHWGLSNVQGWQIAHVH
                     HLCRQLGAPVPSALQPYYNLMNRQPEVELLPAARAFGLGIVPYSPLARGVLTGKYRVN
                     EAADPASRGGRKDRRMMETEWRPESMLIAEKLKAHAETRGVSLPAWAAAWVLNNAAVS
                     SIIAGPRTFEQWTSYFGALDYAWSKEDEALANRLVVTGHASTPGYNDPQYPIVGRFPT
                     IA"
     gene            complement(6043..6270)
                     /locus_tag="VFR00_10410"
     CDS             complement(6043..6270)
                     /locus_tag="VFR00_10410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013418289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HEU0159718.1"
                     /translation="MADKSLDLKGLACPLPILKTRKALSGLPKGATLEVFATDPGSVP
                     DFSAFCESTGNRLLEQEETDGVYRFVIEHVG"
     gene            6488..>8347
                     /locus_tag="VFR00_10415"
     CDS             6488..>8347
                     /locus_tag="VFR00_10415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013947845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEU0159719.1"
                     /translation="MDAKADQPRRSFAFGLERVGFFGINAPYLTFFLIVVFSVLGAMG
                     LMRLRVDDSLSELFRTDTEEFRRYEEIDRRFPSSEYDVLVVVEGKDLLKKPQLEAFRR
                     AIIDLQLADGVDGLVSMLSARGKPDASGYAAPIVPDELPDDPEAYAGIISAMRSNDIV
                     AGKFLSPDGELALAVIALDRKAVQEQSAKLIIGGIAEQLQKELAPVGLKSHLTGAPVM
                     QLEIRNAVERDQILYNGLGLLFGATIAAIFFKRVSLMLLAALPPVLAVLWSLGLLGWI
                     GFKLNLFLNVMTPLVMVMGFADSMQMVSAIRIRLRAGDSKADAVRFAVRIVGPACVLA
                     HGTALLSFFALLFSQSGLIRTFGLAGALAVLISFIAVLLVLPLLGLLLIRNEASLARD
                     RSPADRLMDGLGGLVGWIVDRVVHWPFTTTVVALGSFLFLGHAYLQLEPRYRLADQVP
                     DREQALGATERIDQKLTGANPVHIMIEWQTGDSIYAPGPLRVIAAAHEVLEKEAGLGN
                     VWSLESLRRWLKEAGDDSPETIKKYVGYLPEHLVRRFIAKEGNAVLVTGRLPDVDSSR
                     ILPVVEKIDRALDRLRAANPQFRLSVTGLPAIAARNSARMISQLNESLPICVAF"
BASE COUNT         1414 a         2765 c         2705 g         1463 t
ORIGIN      
        1 catcgtcgcc gccttcaaca ttatttccgg cttgatcatg ctggtgaagg acaagggccg
       61 cgacatcggc attctgcgca ccatgggcgc cagcaagggc gccatcatgc gggtgtttct
      121 gatcaccggg gccagcatcg gcgtggtcgg tacgctcgcc ggcgcgctac tcggcatcgt
      181 gttttgctgg aagatcgacg aaatacgcca gttcgttgct tggctcacca acacacggct
      241 attcgacccc gagatctatt atttgacgcg cttgccggcc gacatcaatc ctcagaccac
      301 ggctgtgatc gtcggcatgg cgctcgtcct ctcggtgctc gccacgctct atccgtcctg
      361 gcgcgcctcg cgcctcgatc cggtcgaagc gctccgctac gagtgaaacc ggtgcaagac
      421 gcaaccccac gcccggtgct cgagctcagg caggttgagc gcaccttcaa gcagggtgac
      481 cgggaaatcc ccgtcctgac cggcgcgaac gcggagcttt acgcgggtca ggcgatcgcc
      541 ttggtgggcc cgtccggcgc cggtaaatcg acgctgctgc atattgccgg cctgttggag
      601 acgcccgacg gcggacgcgt cctggtcaac ggcgtggact gcgcccggct cagcgatggc
      661 gagcgcaccc gcgtgcggcg gcgggaaatg ggcttcatct accaatttca tcaactgctg
      721 ccggaattta ccgcgctcga aaacgtgcta atgccccagc tcatcgccgg tacggcgcgt
      781 cggcgcgggg aagcgcgtgc caaggagctc ttgaccatgc tggggctcgg ccatcgccta
      841 gagcatcgtc ccgcccaact ttccggcggc gagcaacagc gcacggccat cgcgcgcgcc
      901 cttgccaatg cgcccaagct cttgctcgcc gacgagccga ccggaaacct cgatccgcag
      961 acctccgcgc atgtctttca tgagctgatc gagctgatta gacacagcgg ggtggcggcc
     1021 ttgatagcca cccacaacct ggcgctcgcc aagcgcatgc accgcgtctt ggtgctcgac
     1081 agcggccgct tgcaggaggt caatccggcc agcgttccgg gctagcgcca tcccgcggca
     1141 ggccatgccg cgggcggctc acaggcaagc ggcggcatgc ggcaatgcgg cgctaactgc
     1201 gcgcggtgtt ttttgccccc cggcggtaaa aaaaccttta cacacccccc cgcagaccct
     1261 atctcccctt ttcgcaagtt tcgcggcggc ttctgctggt tcgagcagcg cctggtccaa
     1321 ccgactggtg ggggtcccat gagatggaaa gatacgccac tgctgctgcc ctgattggcg
     1381 cacataagcc ggatcgtccg gtgattggcc ttcgtcccca tgcggcggga cgtgcggcac
     1441 gttggttttt gcagaacttc cccggtgacg ttgcctacgc ctacaaggcg aacagctcgg
     1501 tgttcctgct gggcgcgctt tatggtgccg gtgtgcgcca tttcgacgtc gcctcattga
     1561 cggaaatcga ggacgcccag accatccccg aggcccagct gcacttcatg cacccggtca
     1621 agtcgcgctc ggcaatccgg cgtgccttcg ccgagttcgg cgtgcgcagc ttcgccctcg
     1681 acagcgaaga agagctgaaa aaaatcgccg aggagacggg cggctgccgc gacctcacct
     1741 tgtgggtgcg cgtggccgtc tcggccaaaa acagcaagat cccactcgag cgcaagtatg
     1801 gcgttgccgg cgccaaggcc gtgcgcctgc tcggcaaggc acgccaggcg gccaagacct
     1861 tgggcgtcac ctttcatgtc ggctcccaga cggcaacgcc ggatgccttt gtcacggcgc
     1921 tgagcgaggt tggcaggctg atcgtcaagg ccggcgtggt gctcgatcgg ctggacgtgg
     1981 gcggtggctt tccctccatc tactcggacg atgcgcccgc gccccttgcc gcgttcgtgc
     2041 gtgccattag cgattgcgtc gaccgcttgc ccgtgggcga gcattgccgg ctcatgtgcg
     2101 agccgggccg ggccctggtg gctgaggctg aatcactgat cgtgcgcgtc gacgcccgac
     2161 gcggcaacga gctctttatc aacgatggca gctacggcac gctgttcgat gcggcacaca
     2221 tgggcttcgc ctttcccgtg cgcctggtcg gacacgagtt ggagatgggc gaaacgctgg
     2281 tgcccttcgc cttttggggc ccaacctgcg acacgatcga cttcatgccg ggccccttcc
     2341 tgttgccctc cagggtcaag gagggcgact atatcgagat cggcaacacg ggggcctacg
     2401 gccgtgccct cggcgggcgc ttcaacggct atggcgccta cgatgaggcg atcctgctcg
     2461 atgagccctt aataaccatg tacggcgaaa gcgccgtgga aagtgcggcg cagcaggtcc
     2521 aggcgtgagc gcaaagctcg agtatctgtc cgccgcggag agctttctgg atccggttcg
     2581 ggccgacgct gatcagccag cgtcggcccg cgccgttatc gtgccctttg gcctggaggc
     2641 ctcggtcagc tatgggaccg gcacggccgc gggacccgcc gccatcctca aggccagcca
     2701 acagctcgag ctctacgacg acgagctggg ccgggaggcc tatcttgact atggcgtcgc
     2761 cgccctgcgc gagccggcga tcgccatgcc gatcgcggcc gcgctcgagc aagtgcaagg
     2821 cctggtcgag gccgtcgttg ccgccggcaa atttccgttg gtactcggtg gcgagcactc
     2881 cctgaccgcg ggcgccatcc ggcccctgtt ggctcgccac gcggacctgg tggtcctgca
     2941 gtttgacgcg cacgccgacc tgcgcgacgg ctatctcggc gaaccctatt cgcacgccgc
     3001 cgccatgcgc cgggtgttgg accacgcggg cgtatccctg atccaggtcg gtctgcgctc
     3061 cgtcgccgcg gccgaggcgg cctatatggc tcagcaccgc gaccggatca gcgtctactg
     3121 ggccaaggac caggcggcct gggacatcga tggcctcgtg cggcaactgg gcggccgtcc
     3181 gctctacgtc agcttcgata tcgatgcgct ggacgccgcc gtcatgccgg ccacgggaac
     3241 ccccacccct ggcggcctcg gctactggca ggccctggcc ctgttgcgac gtgcctgtgc
     3301 cgcgggccgc gtggtgggcg ccgatctcgt cgagttcgcg ccgatcgcct cactgcacgc
     3361 ctacgattac accgccgcgg cgctggccta caaattgctg aactatgcgc tctgcggcgc
     3421 tgagcgaccc ggctaacgcg tcaggcttcg catcaggccg cgcagcgcct ccgggtgatt
     3481 gtgcagccgc cagcgcgagg caaagcccgc aaccaggatg agcacaccat tgatcgtcag
     3541 cgtggtggtc aggccgatgc gttcggcgca gtagccggcg atcagcccgc cgatgggcgc
     3601 ggtaccggca aacgacatgc tgtaaaggcc gatcacgcgc ccgcgccagg cgtccggaac
     3661 cgattgttgc agcatggcat tggtggcgtt gacgccaagc agcgccgccc cgccgagaat
     3721 ggccagcaag accagggaaa tgggcagcgt gcgtgaccag gcaaagagcg tcaaggcggc
     3781 gccgaccgcc aggggcgcca aggtcatgag ccgaatctgc cggccgtagc ccggctgcaa
     3841 ggaaagatag gtcgccgacg ccaaggctcc aatcccggcc gagctcatga gcaggccgag
     3901 ggtcgaggat tgcccgtcga aaaagtggct gacaatcgac ggcatcagcg gcacgtaggg
     3961 tgttgccagc aggcccaagg cgacgcacac gggcaggaga tagcgcacgg ccgggaaggc
     4021 aaacaacacc tgcaggcttt ggccgaaggt ctgctgggcg cccgccgcgg cggtggcgcc
     4081 gcgcggctcg ggcggcaggc gcatggccaa cagggccgcg ataatcgccg catagctcat
     4141 ggcattgagg gcaaagcacc aggcctcgga caggtaggcc agcacgatgc cggcgatggc
     4201 ggggcccagc atgcgcccga cattgaagac catggcgctc atgccaagcg cgctcgtcac
     4261 caaggcccgc tcctcgacga tgatgcggat cagcgtctgt cgcaccggca tttcgaaagc
     4321 attggccgcc ccgatcaggg ccgcgatcgc cagataggcc gtcaccgtct cgacgcctgc
     4381 cgcggccatg ccggcgagca gcacggagag gctggccagg aggagatcga tgatgatcag
     4441 gcttttcagc cgcggcaggc gatcggcagc aagcccggcc acgggcccca gcaacaaata
     4501 gaagatgttg aggagaaagc cggcaacccc gagcaggaag gccgagttcg tcagccggaa
     4561 caccagccag gcgatggcaa cctgctggat ccaaccgccg atcacggaga cggcgtggcc
     4621 tgccacatac aaccgaaaac ggggcttact cagcgccggg ggcagccaca agtgccagtc
     4681 tgccaccccg cccgctcccc accgaccgcc cttctcactc agcggcccaa gcctaagcgg
     4741 tgagaaaggg gcgcgacaag gcccccggcg gcaagcgtga gcctcaaaac tggtgggcgc
     4801 gacagggatc gaacctgtga cccctcccgt gtgaagggag tgcgctaccg ctgtgccacg
     4861 cgcccttggc cggacttagg cgagcctatc cgcccctgtc aagccgctgc cagggcctgc
     4921 cattggcgag gagacccggg ccagcccgcg cccgttcagg cgatcgtggg gaagcggccg
     4981 acgatggggt attgcgggtc gttatagccg ggcgtggagg cgtggcccgt aacaacaagg
     5041 cgattggcca aagcctcatc ctccttgctc caggcatagt cgagggcgcc aaaatagctg
     5101 gtccactgct cgaacgtgcg cggcccggcg atgatcgagc tcaccgcggc gttgttgagc
     5161 acccaggccg cggcccaggc ggggaggctc acgccccgcg tctcggcgtg ggcctttagc
     5221 ttctcggcaa tcagcatgct ttcggggcgc cactccgtct ccatcatgcg ccgatccttg
     5281 cgcccgccgc ggctggcggg atcggcggcc tcattgaccc gatacttgcc agtcaacacg
     5341 ccgcgcgcga gcggactata gggcacgatc ccgagcccga aggcgcgggc ggcgggcagc
     5401 agctccacct cgggttggcg gttcatcaaa ttgtaatagg gctgcagcgc gctggggacg
     5461 ggagccccca gctggcggca aaggtggtgc acgtgggcga tctgccagcc ctgcacgttg
     5521 gagaggcccc agtggcggat cttgccgcta cggatcaggt ccgagaaggt ttgaacgacg
     5581 ttttcccagg ccgtgtcggc atcgacgcgg tggatatagt aaaggtcgat acaatcggtc
     5641 ttgagccggc gaaggcttcc ctcgaccgca tccatgacat ggcgccggga aaggccgctg
     5701 tcgttgggac ccgcgcccag cttgttgcca accttgctgg cgatgatcca ggcgttgcgc
     5761 ttgccctgca cggcgtcccc gagcacgctt tccgagcgcg tctcgctata gacgttggcg
     5821 gtatcgacaa agttgacccc ctgctcgagg gcgtaatcga gcatgcgccg cgcctcggcc
     5881 gccccggccg ggccgccgaa catcatggtg cccagcgaga tttccgagac cttgaggccg
     5941 ctcacgccca gcgggcgata gcgcatcttt gccatccgcg aagctctcct cgatcccttg
     6001 tgccttccct tttgcctggg ggctgcgttt ggcgcgacgg tctcatccca cgtgctcgat
     6061 cacgaagcgg tagacgccgt cggtttcctc ctgttcaagc aggcgatttc ccgtgctttc
     6121 gcagaacgcg ctaaaatccg gcaccgagcc cggatcggtg gcaaaaacct ccaaggtggc
     6181 ccctttgggc aagccagaca gcgccttgcg ggtcttgaga attggcagcg ggcaggcgag
     6241 ccccttcaaa tccaggcttt tgtcggccat gcgtgaggct ccaggcagga tcgcgccgcg
     6301 cgcgtgcgcc ctcagatatc aagccaggcc ggcaactggc aacaatgcca ggcaaattgc
     6361 aaggggtgct tgcgtgccag ccgcgcccac gccacgatga tctgaaagac gagattctct
     6421 gtcccggcgc cggggtgagg ggcagaaggc gcgccttgac gggtgccttg tcgctgggga
     6481 cgggagaatg gacgccaagg ccgatcaacc gcgacgctcg tttgcgttcg ggctggagcg
     6541 cgtcggattt tttggcatca acgcgcccta cttgaccttc tttctgatcg tcgtcttttc
     6601 cgtgctcggt gccatgggcc tgatgcggct ccgggtcgat gattccctgt cagagctctt
     6661 tcgcaccgat accgaggagt ttcggcgcta tgaggagatc gatcggcgct ttccttcgag
     6721 cgaatatgac gtgctggtgg tggtggaggg caaggacctc ctgaagaagc cgcagctgga
     6781 agccttccgc cgtgccatca tcgatctgca gctggccgac ggcgtcgatg gcctcgtttc
     6841 catgctctcg gcacgcggca aacctgacgc cagcggctat gccgcgccga tcgtgcccga
     6901 cgagttgccc gacgacccgg aggcctatgc cggcattatc agtgccatgc gcagcaacga
     6961 catcgttgcc ggcaagttct tgtccccgga tggggagctg gccctggccg tgatcgcgct
     7021 cgaccgcaag gcggtgcagg agcagagcgc caagctcatc attggcggta ttgccgagca
     7081 gctgcagaaa gagctggctc cggttggcct caaatcgcac ttaaccggtg cgccggtgat
     7141 gcagctcgag atccgcaacg ccgtcgagcg cgatcagatc ctttacaacg gactgggact
     7201 gctgttcggg gccaccattg ccgccatctt cttcaagcgc gtgtcgctga tgttgctggc
     7261 tgccctgcct cccgtgctgg ccgtgctctg gtcgctgggg ctgctcggct ggatcggctt
     7321 caagctcaac ttgttcctca acgtcatgac gccgctcgtc atggtcatgg ggtttgccga
     7381 cagcatgcaa atggtctcgg caatccgcat ccgactgcgg gcgggtgaca gcaaggccga
     7441 cgcggtacgt ttcgccgtgc gcatcgtcgg ccccgcctgc gtcctggccc acggcaccgc
     7501 gctcttgtct tttttcgcac tcctgttctc gcaatccggc ctgatccgca cgttcggact
     7561 agccggtgcg ctggcggttt tgatttcttt catcgccgtg ctgttggtgc tgccgctgct
     7621 cggccttctg ctcattcgca atgaggcctc cctggcacgc gacaggtcgc ctgccgaccg
     7681 gctgatggat ggcctcggcg gattggtggg ctggatcgtc gaccgggtgg tgcactggcc
     7741 cttcaccacg acggtggtgg cgctgggctc atttctgttc ctcggccatg cctatctaca
     7801 actcgaaccg cgctaccgct tggccgatca ggtgcccgat cgggagcagg cgctcggggc
     7861 gaccgagcgc atcgatcaaa agctgaccgg cgccaacccc gtccacatca tgatcgaatg
     7921 gcaaaccggg gactcgatct acgcccccgg cccgctcagg gtgatcgcgg ccgcacacga
     7981 ggtgctcgaa aaggaggcgg ggctcggcaa cgtctggtcg ctcgagagcc tgaggcgctg
     8041 gctcaaagag gccggcgacg acagccccga aaccatcaaa aaatacgtgg gctacctgcc
     8101 ggaacacctg gtgcgccgct ttatcgccaa agaaggcaat gccgtgctgg tcaccgggcg
     8161 gctgccggac gtggattcca gccgcattct accggtggtg gagaagatcg atcgggcgct
     8221 cgatcgcctg cgcgcggcca acccgcagtt tcgcctgtcc gtcacgggtc tgccggcgat
     8281 cgctgcccgc aacagcgcgc gcatgatctc ccagctcaac gaaagcctgc ccatctgcgt
     8341 tgccttc
//
LOCUS       DASTMT010000448        18739 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_4188378, whole genome shotgun sequence.
ACCESSION   DASTMT010000448 DASTMT010000000
VERSION     DASTMT010000448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 18739)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18739)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18739
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4188378"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..385
                     /locus_tag="VFR00_10420"
     CDS             <1..385
                     /locus_tag="VFR00_10420"
                     /EC_number="4.2.1.84"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011473192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nitrile hydratase subunit alpha"
                     /protein_id="HEU0159720.1"
                     /translation="HEMGYGGEEGHTMMVVANTPEVHNVIVCTLCSCYPWPVLGLPPV
                     WYKSAPYRSRAVLDPRSVLAEFGVSLPANKQIKVWDSTAEIRYLVLPERPAGAEAWSE
                     EELARLVTRDSMIGTGLARSPSEVS"
     gene            382..1041
                     /gene="nthB"
                     /locus_tag="VFR00_10425"
     CDS             382..1041
                     /gene="nthB"
                     /locus_tag="VFR00_10425"
                     /EC_number="4.2.1.84"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011158357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018822 - nitrile hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0050898 - nitrile metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrile hydratase subunit beta"
                     /protein_id="HEU0159721.1"
                     /translation="MNGAQDLGGMMGFGPVTAEPDEPVFHSDWERRAFALTLAMAAPG
                     GWNIDQSRAARESLHPAEYLAKSYYEIWLAGLEKLMAERGLVAPAEIDAGKGLEPAKT
                     VPRVLTAARVERVLARGGPTERVAPAPQRLRIGDRVRARNIHPLGHTRLPRYVRGHVG
                     HVTHVHGAHVLPDANAAGAGENPQWLYTVRFAAQELWGEAADPEVSVSVDAWESYLEP
                     A"
     gene            1065..1409
                     /locus_tag="VFR00_10430"
     CDS             1065..1409
                     /locus_tag="VFR00_10430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012044150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0050898 - nitrile metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrile hydratase accessory protein"
                     /protein_id="HEU0159722.1"
                     /translation="MRDLPDDVAAPVFQAPWEAQAFALVLSLYDKGLFTWPEWAATLA
                     QEIKAAEAAGDPGRGDTYYQHWLGALEKMLARKGVTTPQALGRYHKAWNAAAERTPHG
                     APIELRTEDFGG"
     gene            complement(1429..2676)
                     /locus_tag="VFR00_10435"
     CDS             complement(1429..2676)
                     /locus_tag="VFR00_10435"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018044686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HEU0159723.1"
                     /translation="MSMTTARARSRRQFLRFLAASPLLPWASRSALGQTAPAQRLPDP
                     MLWAPRELETLITHPKEAINVFDFEPVARKTLPPAHFGYLVAGVDDEVTLRANREGFL
                     KFQLRPRRLVDVSTIDMRCSILGSAYDSPIFICPTASNRAFHPDGEVAVAKAARVGNH
                     LQMLSTVATTSIEEATSARGVPVWFQLYASPRWQIAEALVKRAEQAGTPGIVVTVDDL
                     GGRNQETLARLRRSDTRQCSSCHAPGWGYVARKPNYDGLDMSGVSTVLAPNMTWEIVK
                     RLRDTVRSKLLLKGIMSHEDARLAAANGIDAIIVSNHGGRSDDSGRSTIDALPEIIEA
                     AGSMPVLVDSGFRRGSDIVKALALGAKGVGIGRPCLWGLGAFGQEGVERVLELLRAEL
                     RVAMQQLGAPSLKDLTPAMVRRA"
     gene            3610..4578
                     /locus_tag="VFR00_10440"
     CDS             3610..4578
                     /locus_tag="VFR00_10440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018453345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEU0159724.1"
                     /translation="MRRWIERVCLLGLLLWASPGEAQDYPNRPIKFVVPFPAGGPADT
                     ISRILTDKMASLIGQTMVVENRAGAGGLTGIASVAKAEGDGYTIAIAPSSGLAMNVNL
                     RENMPFQPLKDLALITQIVSVPEILVVNEKVPANSLTELVALAKAQPGKLVFASTGLG
                     GMPHLAMELFKLSAEVDLVHVPYTGAAPAVNDLLGGHVQVMFADVPVLLGSIQSGKLR
                     ALAIGSKTRLASLPTVPTTAEVGMPQVEADNWYGLVAPIATPEAIITKLHAAAAQALH
                     SDEVREKLAAQGALAVGNSSAEFASYVRSEIERWGRVIAATGLKIK"
     gene            complement(4585..5463)
                     /locus_tag="VFR00_10445"
     CDS             complement(4585..5463)
                     /locus_tag="VFR00_10445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011089101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU0159725.1"
                     /translation="MSKIPQQQIPGVYHHRVGDIVVTALSDGFLDGSLDVLRNISQDE
                     ARQILSDNFRPARRTQVNAFLVYSAGRLALIETGSGNYLLPTAGKLLANLQAAGVAAS
                     EIEAVLLTHMHPDHSAGLTNMETGKRNFPNAELVVHENEPRHWFDDAKMAQATERAKK
                     LYFQCAREQTTPYKERTRLFTAGEIFPGVTAIPRPGHTPGHTTFLISSGKDQLLIWGD
                     IVHVPEVQTARPEVCMEFDTDAEAAAAQRRKVFDMVATDRLLVTGMHLHFPGFAHLVR
                     RGTGYHLIPAAWEHAL"
     gene            complement(5509..6144)
                     /locus_tag="VFR00_10450"
     CDS             complement(5509..6144)
                     /locus_tag="VFR00_10450"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HEU0159726.1"
                     /translation="MDQAHTSMTLFGSSRSPFVRKVLVAAHESGLAERLSVTRVVVAA
                     NKPNAEVMAANPLNKIPTLLLADGTALFDSRVICEFFDTLHAGPPLLPTEPAARWRVR
                     RLEALGDGLMELNIARLGEMNRPTGGQSATHLSSFARKISATLDHLEGQCGDLLGSFQ
                     LGGIAVACALAHLDFRFAAEDWRARRERLGAWYAEVAARPSMRATEYADIY"
     gene            6296..6676
                     /locus_tag="VFR00_10455"
     CDS             6296..6676
                     /locus_tag="VFR00_10455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011471542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HEU0159727.1"
                     /translation="MPAPAPSPLATYLAHLERGQLAYQFSPQAGRAVFYPRLVCPFTG
                     SDQLEWRVSKGLGTVYSTTVVHPQEGAPYNVALIDCDEGFRLMSRVEGVAPQQVRIGM
                     RVEFRAHIPGGEAPPMPVFVAVEA"
     gene            6680..7834
                     /locus_tag="VFR00_10460"
     CDS             6680..7834
                     /locus_tag="VFR00_10460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002716937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HEU0159728.1"
                     /translation="MQGLKRASAAIVGVAESDLGQVADGLSVIDLMAQGTKRALDDCG
                     LALRDVDGLFSATAQSRLSVLAFAEYLGIDPPCLGSTIVGGSSFEYHVAHAMAAIEQG
                     LCSVAVIAYGSTQRTIGRKQASQREINPYETPFKPFLPASAYALAAMRHMHQFGTTRE
                     QLAAVAVAAREWALLNPAAWEKKPLSIEGVLSSRMVSYPFTVRDCCLVTDGGGAIVVT
                     SAERARALKKPPAYVLGCGQSVTHASISNMPDLTVTGAVASGQAAYRMAKLGPADVDV
                     LELYDAFTLNTVLFLEDLGFCKKGEGGAFVSEGRIAPKGSLAVNTNGGGLSYCHPGMY
                     GLFLLIEAVRQLRGECGARQVADCEVAIAHGNGGVLSSQATVILGTPATL"
     gene            complement(7851..10172)
                     /locus_tag="VFR00_10465"
     CDS             complement(7851..10172)
                     /locus_tag="VFR00_10465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0159729.1"
                     /translation="MRPLLVAAAAIVSCAPLNPAAAEPVLDRALSGLRLFSPSPTCTL
                     VKIEFNFRIRYVSHFPLATGEELRINVRAIDPTQAAALAILRREALRPPASAAAAIRA
                     IEFELDQPAGPVLRVQFLHPVAYQVAPGADFESLVIAIAGKTPQAACQPRFPSRLGAG
                     WDTTVSHGDWAPNLNPAKPGGPLPPPPVRPKGRAAGALAPAELSAVAAAMDEARAALK
                     KGNYAQAIELLSKVLTRPENERSAEAQELLGIAYQKAGHIGEARAQYEDYLSRYPNAE
                     GSEGVRQRLDGILTASSEPGERLRSGKAQPGEPGGPPRSGTTWMVSGSASQFYIRDDS
                     FRTLRDPSLPPNPNEDKDAHLTHQNSLLSSFDLSAVWSNDQFKSKFRFSGTEEHKFDD
                     NREIVSVAALFFETTIKQWDLMTRLGRQTRNTGGVVGRFDGGVVSWQANPNVRVNVVG
                     GSPVERRADEPFKDERYFYGASVDFGHFWGNLEATLFAIEQRDRSILDRQAVGGELRY
                     IDANKSAFALLDYDVHFQELNAAMFSGSWTLPDKSTISAGIDYRKSPYLTAWNALQGQ
                     PFLTLYDMLKLHSKDEVDQFALDRTPTFKSATLGYAFPLGKNYQVNLDATASSLSGTV
                     ASGGIEAMLPTGTELYYSAQLTGTNLFAEGDMYIAGVRMADLEASNLYVLDLSARYPL
                     SNELRINPRLRLGYQVGATTDLREITVLPSILINYYWTRDLSLELEIGAKWTERQQAG
                     ATQNETDLFFTAGFRYDFYADGQKPCAFVTTCR"
     gene            complement(10267..12126)
                     /locus_tag="VFR00_10470"
     CDS             complement(10267..12126)
                     /locus_tag="VFR00_10470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0159730.1"
                     /translation="MNALLRAIAILLCFGMAGAELLGTRAEAQTRAFDHFTTGFPLLG
                     SHYSVDCTSCHVNGRFKGTPRQCIGCHNGSIAPGQRSTHGPTTANCEACHTTATWAQV
                     RFNHNQIIGACASCHNGSHARAKPPTHIDTTAACETCHKSTFSFLAARWDHNGITGNC
                     ASCHNGSKALGKSKSHIATSAPCETCHKSTTSFAGARFDHPVGVSNCASCHNGATALG
                     KPATHIVTSAPCETCHKSTTSFAGAQFVHTNVTTGCASCHNGTTALGKSAKHIPTTAA
                     CETCHKSTVSFAGTAMNHSGISSGCATCHNGSSAVGKSAKHIPTSAACETCHKSTASF
                     VGTAMNHTGITTGCASCHNGSMALGKPATHVATSASCEMCHKSTVSFAGAQFVHTNVT
                     TGCASCHNGTTALGKSAKHIPTTAACETCHKSTVSFVGTAMNHAGISSGCATCHNGTM
                     ALGKPAKHVATSAACETCHKSTVSFAGAAMNHTGISSGCAGCHNGTTALGKPAKHVPT
                     TAACETCHKSTVSFVGAVMNHTGITTGCAGCHNGTTALGKPATHIPTTQPCETCHRST
                     VSFAGTRMNHTGIVSGCATCHNGVYAIGKPRSGHPNTTAPCENCHRNTRSFGD"
     gene            complement(12731..13573)
                     /locus_tag="VFR00_10475"
     CDS             complement(12731..13573)
                     /locus_tag="VFR00_10475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0159731.1"
                     /translation="MSVVSDEVLMAFADGALDPAERDAVEALLQRHPECRQKVEKFRT
                     TLSPLQNLFRETIRIDHLGPLIDQIRRADLAAGQTSPRTAEVRQLPQARPTRAPRHPW
                     HQHYPMALAASLALLIGAALGSLLQPRATSSVPTAGFIGGSDGQLTAQGPLQELLEHT
                     ARGIPVRAELAAGGTWELETAFTFRRSGDQQPCRRYELRSTAQEHFAGFACRGKDGQW
                     AVQAHLKLQAMPANPGPGYAPASGPNVDAANAALEAAIRVASGGKVYPDAEEKAAIVA
                     GWVR"
     gene            complement(13554..14213)
                     /locus_tag="VFR00_10480"
     CDS             complement(13554..14213)
                     /locus_tag="VFR00_10480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEU0159732.1"
                     /translation="MKLTTGSKASLAAAGNRGTPRQGIGGPPARYVAGPQGEAVGDDF
                     RERMIAFLPRLAGFAYALTGNADQRDDLVQETCARALANRDKWQPGTRLDSWMFRIAQ
                     NLWLDRKRADRFRGESIDVDAVDTLVGSDGRAVVENRLELAAVLRGLEQLSPEHRVLI
                     ALVCVDGLTYQEAAEVLKVPVGTVMSRLARARLAIHDAINGAPAKATTTMETRRVRSV
                     R"
     gene            complement(14210..15514)
                     /locus_tag="VFR00_10485"
     CDS             complement(14210..15514)
                     /locus_tag="VFR00_10485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016920452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HEU0159733.1"
                     /translation="MARAQGIFEKLVMPGPLIGAHAELEKTCKSCHEPFAQKSQSGLC
                     LACHKPIAADRQAKRNYHGRQPEALGSECKACHTEHKGRTFDIVQLNRETFNHAFANF
                     TLTGSHKRASCDGCHVKNTKYRETPGRCVDCHRSIDPHKGRLGEKCDGCHGEEAWRRV
                     KPFDHSKTRFALLAAHKQVACGKCHAGERYQGLATTCSSCHGLQDKHAGRNGSACESC
                     HTPTKWAAVTFDHNKATRFPLRGAHAKLACERCHSGEPRRDKLATTCAGCHRKDDPHK
                     GQLGAACETCHKETGWRQAVTFDHDLTRFPLIGLHAAVACEACHRTPSFKEASRACAG
                     CHTDAHHAGRLGPNCATCHNPNGWSRWRFDHDKQTRYALTGAHRALQCHGCHTTRAVT
                     KVSAPDTCYACHGKDDVHDGAFGRACEKCHTTVSFRQGLSRQ"
     gene            complement(15586..16596)
                     /locus_tag="VFR00_10490"
     CDS             complement(15586..16596)
                     /locus_tag="VFR00_10490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008972354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridine nucleotide-disulfide oxidoreductase"
                     /protein_id="HEU0159734.1"
                     /translation="MTASASLPMRRRAEAAPTAPSPAAKSTARAPKPAAAAGKAEPAA
                     PRPAPPKTAAPEPTLGSRLASLGFASLLAAALIYGWQMRDEGHLTPEAGLGYWLGILG
                     ASAMLLLLGYPLRKRLAGLQLLGSVSGWFRLHMLLGVIGPALILLHANFKLGSLNSNV
                     ALLAMLTVAGSGLIGRYLYGRIHLGLYGRRAEIAELQADVAALKDAIEGELSMPADVL
                     AALDRHAERARRRRGALTSLFAFLALRLASLGLKARLSQLAARHVGAEAKRLGWSRRL
                     RRRHGKAARRLIEDYLAAVNKTAAFVFYERLFALWHVLHLPLFALLLFAAVIHVVAVH
                     LY"
     gene            17004..18257
                     /locus_tag="VFR00_10495"
     CDS             17004..18257
                     /locus_tag="VFR00_10495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0159735.1"
                     /translation="MSPSLVMTLALVGGTVAALVVVVARAAASPSSELSHALAQLGVV
                     RLKELERTWRGRTDEARIELLRQLLEEARSSRAWRWPSRGKPTVLVAGSLLALLLCSL
                     TLLGSADAPLATPRVPEGTGWLSQDPDVARLELYAKTKAPRPAASAVAAKTAELPDVD
                     TMIERLALRLQKKPDDAEGWRMLGWSFFNVQQPAKAVEAYARAVALEPQGAALKSAYG
                     EAMVATENGTVTPPALETFNAALALEPNNAKARYFVALALEQSGKKKEALDRWLGLLA
                     EPSDNEPWAGELRQRAPALARELGVSLAVPALAAATTSDGATPGAAKPRALTAAEMRL
                     AQALPAEQRQAMIRSMVSGLADRLESTPRDEEGWLRLIHSRLQLGEDDAARAALARAL
                     AVFADDASAGGRITAAAKELGLANN"
     gene            18283..>18739
                     /locus_tag="VFR00_10500"
     CDS             18283..>18739
                     /locus_tag="VFR00_10500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008972352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEU0159736.1"
                     /translation="MPLAFDPILAFYAVPLVVLWTVYVGVRKKVDARSRSTQAEAQEA
                     GLTEPASLHPLIDPVACLGCGACVRACPEVANGHPVLGMIDDRAELIEPASCIGHGAC
                     KTACPTGAITLVFGTETRGVELPIVNPDFQTNVPGLFIAGELGGMGLIKN"
BASE COUNT         3097 a         5907 c         6449 g         3286 t
ORIGIN      
        1 ccacgagatg ggctacggcg gcgaggaggg ccacacgatg atggtcgtgg ccaacacgcc
       61 cgaggtgcac aacgtgatcg tctgcacgct gtgctcctgc tatccgtggc cggtgctcgg
      121 gctgcccccg gtctggtaca agtcggctcc ctatcgctct cgcgccgtgc tcgacccgcg
      181 ctcggtgctc gccgaattcg gcgtgagcct gccagccaac aagcaaatca aggtttggga
      241 ttcgaccgcc gaaatccgct acttggtgtt gcccgagcgg ccggcgggtg ccgaggcttg
      301 gagcgaggag gagctcgcac ggctcgtcac ccgcgatagc atgatcggca cgggactagc
      361 gaggtcgccc tcagaggtgt catgaacggc gcacaagacc tgggtggcat gatgggcttc
      421 ggccccgtca cggccgagcc ggacgaaccg gtcttccact ccgactggga acggcgcgcc
      481 ttcgcgctga ccctggcgat ggctgccccg gggggctgga acatcgatca atcccgggcg
      541 gcgcgtgaaa gcctgcatcc ggccgagtac ctggcgaagt cctactacga gatatggctc
      601 gccgggcttg agaaactgat ggccgagcgg gggctcgtgg cgcccgcaga gatcgatgcc
      661 ggcaagggct tggagcctgc caagacggtg ccgcgcgtac tgacggcggc gcgggtggaa
      721 cgggtgctgg cgaggggcgg gccgaccgag cgcgtcgcgc cggcgccgca acgtctaagg
      781 atcggtgacc gcgtgcgcgc ccgcaacatc caccccctgg ggcatacccg cctgccccgc
      841 tacgttcgcg ggcacgttgg acacgtcacg cacgtgcacg gcgcgcatgt cttgccggat
      901 gccaatgccg caggcgcggg cgaaaacccg caatggctct atacggtgcg gtttgcagcg
      961 caggaactgt ggggagaggc ggcggacccg gaggtttcgg tctcggtcga cgcctgggaa
     1021 agctacctgg agcccgccta gtgccgccaa acccatcccc gcccgtgcgc gatctaccag
     1081 atgatgtcgc cgctcccgtg ttccaggcgc cctgggaggc gcaggccttc gcgctcgtgc
     1141 tcagccttta cgacaagggc ctgttcacct ggcccgagtg ggccgccacc ttggcgcagg
     1201 aaatcaaagc tgccgaggcg gccggggatc ctggtagggg cgacacctat taccagcatt
     1261 ggctgggcgc actggaaaaa atgctggcgc ggaaaggcgt gacgacaccg caggcgctgg
     1321 ggcgttacca caaggcctgg aacgcggcgg ccgaacgaac cccgcacggt gcgcccatcg
     1381 agctcagaac ggaagatttc ggcggctgag gcggcggcga tcgagccctc aggcgcggcg
     1441 taccattgcc ggggtcaggt ccttgagaga tggtgcgccg agttgttgca tggcgacgcg
     1501 cagctcggca cgcagcagct ccagtacgcg ctccacccct tcctgaccga aggctccaag
     1561 gccccataga cagggccgcc cgatgccgac gcctttggct ccgagcgcaa gcgcctttac
     1621 aatatcgctg ccgcggcgaa atccgctgtc caccagcacc ggcatcgacc cggctgcctc
     1681 gatgatctcg ggcagcgcgt cgatggtcga gcgcccgctg tcgtcactgc ggccgccatg
     1741 gttggacaca ataatggcgt cgatgccgtt ggccgccgcc agccgcgcgt cttcgtgtga
     1801 catgatgccc ttgagaagga gcttgctcct gaccgtgtca cgcaggcgct tgacgatttc
     1861 ccaggtcatg ttgggggcca ggaccgtgga cactcctgac atgtcgagcc cgtcgtagtt
     1921 gggcttgcgc gcgacatagc cccagcctgg cgcgtgacaa ctggagcact gtcgcgtgtc
     1981 gctccgccgc aggcgcgcca aggtctcctg gttgcggccg ccaagatcat cgaccgtcac
     2041 cacgatgccg ggggtgccgg cctgctcggc acgtttgacc aaggcctccg cgatctgcca
     2101 gcgcggcgat gcgtaaagct gaaaccagac cggcacgccg cgcgcgctgg tggcctcctc
     2161 gatcgaggtt gtggcgaccg ttgagagcat ctgcagatgg ttgccgaccc ttgccgcctt
     2221 ggccacggcg acttcgccgt cgggatggaa ggcgcggttg ctggcggtcg ggcaaatgaa
     2281 aatcgggctg tcgtaggcgc tacccaggat cgaacagcgc atgtcgatcg tgctgacgtc
     2341 gacgaggcgc cgcggccgca gctggaactt caggaagcct tcgcgattgg cgcgcaaggt
     2401 gacttcgtcg tcgacgcccg cgaccagata accgaagtgc gccggcggca gagttttgcg
     2461 cgccacgggc tcgaagtcga acacgttgat ggcctctttg gggtgggtga tcagggtctc
     2521 cagctcgcga ggggcccaca gcatgggatc gggcagtctc tgggcgggcg cggtctgccc
     2581 caaggcggat cgactagccc agggcaggag cgggcttgca gccagaaagc gcaggaattg
     2641 gcggcggctc cgagcgcgtg cagtggtcat ggacattgag aggctcctaa ggccaagctg
     2701 gatcttagcg caggcgcagc tcgagcgcgc cccgcgatgc gtcgtaggcc atccacgctg
     2761 gcgaggcggg ccgttgtggt cgctgtccgc tgccgcggtc gcgtcgcgcg cgaggcgccg
     2821 ttgcaacgcg cctgcgttcg ggggcgttgg tcgctgatac atggcgggct cgccggacga
     2881 gtcgacgcaa aaagcatacc gtctcgctcc gcttcgacgc aaagcgacgc ctaagtgcgc
     2941 cgcacggcgg agtaaaattt ctctttctgt cgcgccgccg ctgctgcgaa gcggattttt
     3001 ctgtatgctg tgattcggag cggcgctcgg cccttcgtct cgattgacta ccccgcaccg
     3061 caatcgagcc gacagctgca gcaggctcag taccgctccc caatcccctt gctccgtcaa
     3121 cttcggcgcg cgcggcagac gcagccgagc ctgcctttgg cagcgcggcc gcctgccacc
     3181 agcatgggtc gttgcagcgc gcgacgacgg tcgtgctcgg gcgctcatat ggcttgtcat
     3241 tcggcgcgat ggcatgtgac gccgacgcac gcgcagggct cgacggtgtg ttgaggcacg
     3301 caaatgtcgc ggccggctgg cgtcttgcgg atccgttccc cgtgttgcgg acatcgcgcg
     3361 cagacgcacg cgtgtcgccg ctcgagaggt tagggaggag ggaagctaaa gcctccctcg
     3421 ccccgggtcc gcatctgtgg accctgcagc aaccccgcag tgctgcgctc ccatcgagag
     3481 cggggaagcc tgcatgttaa gggcaaacgg caaggagcgc cagtgccatc tgaggccaag
     3541 gcgcgcgcag acagaggccg ccgttgtgcc atactgggcg agacaccatt aggcttggag
     3601 gcgctgtcga tgcgcagatg gattgaacgt gtttgtctgc tcgggctgtt gctgtgggcc
     3661 tctcccggcg aggcccagga ctatcccaat cggcccatca agttcgtcgt gccgtttccg
     3721 gccggcgggc ccgccgatac catcagtcgc attctgaccg acaagatggc gagcctgatc
     3781 ggacaaacca tggtggtcga gaaccgcgcc ggtgccggtg gcctcaccgg cattgcctcg
     3841 gtggccaagg ccgaggggga cggctacacc atcgccatcg caccctcgag cgggctcgcc
     3901 atgaacgtca acctgcgcga gaacatgcca tttcagccgc tcaaagattt ggcactcata
     3961 acgcaaattg tttcggtacc ggaaattctg gtggtcaatg aaaaggtgcc ggccaactcg
     4021 ctgacggagc tcgttgccct ggccaaggcg caacccggca aactcgtgtt cgcctcgacc
     4081 ggtctcggcg gcatgccgca tctggccatg gagctcttca agctcagcgc ggaagttgat
     4141 ctcgtccacg tgccctacac gggggcggcc ccagcggtca acgatctcct cggcgggcac
     4201 gtgcaggtga tgtttgccga cgtgcccgtg ctgctcggca gcatccaatc gggcaagttg
     4261 cgggcgctgg cgatcggcag caaaacgcgc ctggcgagcc tgccaacggt gccgaccacg
     4321 gccgaggtcg gcatgccgca ggtggaagcc gataactggt acgggctggt ggcgccgatt
     4381 gccacgcccg aggcgatcat caccaagctg cacgccgcgg ccgcacaggc cctgcactct
     4441 gacgaagtca gggaaaaact cgccgcgcaa ggtgcgcttg ccgttggcaa ttcgtcggcg
     4501 gaattcgcaa gctacgtcag gagcgagatc gagcgctggg gtcgcgtgat cgccgccact
     4561 ggcctcaaga tcaagtaggc tactttataa ggcgtgctcc caggcggcag ggatcaagtg
     4621 atagcccgtg ccccgccgga ccagatgcgc aaagcccggg aaatgcaggt gcatgcccgt
     4681 gacgagcagc cggtcggtcg cgaccatatc gaacactttt ctgcgttggg cggcggccgc
     4741 ctcggcatcg gtatcgaatt ccatgcacac ctccggtcgt gcggtttgca cctccggcac
     4801 gtggacaatg tcgccccaga tcagcagctg gtccttgccg gaactgatca ggaaggtggt
     4861 gtggcccggt gtgtgcccag ggcgtgggat ggcggtgacg ccgggaaaga tctcgccggc
     4921 cgtgaatagg cgcgtgcgct ccttataggg cgtggtttgc tcgcgcgcgc attgaaaata
     4981 gagcttcttg gcgcgttcgg tcgcctgcgc catcttggcg tcgtcgaacc aatggcgcgg
     5041 ctcgttctca tgcacgacga gctctgcgtt gggaaagttg cgcttgcccg tctccatatt
     5101 ggtgaggccg gcggagtgat cgggatgcat atgggtcagg agcacggcct cgatctcgct
     5161 cgcggcaacg ccggcggcct gcaagttggc gagcaactta ccggcggtgg gcaaaagata
     5221 gttccccgag ccggtttcga tcagggcgag ccgaccggcc gaatagacca ggaaggcgtt
     5281 cacttgcgtg cgccgggcgg gacggaagtt gtcgctgagg atctggcgcg cctcgtcctg
     5341 cgaaatgttg cgcaaaacgt cgagggagcc gtcgagaaag ccatcgctga gcgccgtgac
     5401 gacgatatcg ccgacgcggt gatgatagac gccggggatc tgctgctgtg gaattttgct
     5461 catgctggga tgtcctcgcc cgattgcaac gtcactcgcc caagcctttc agtagatatc
     5521 ggcatattcg gtggcgcgca tggagggacg cgccgcaacc tcggcatacc aggcccccag
     5581 gcgctcgcgc cgagcgcgcc agtcctctgc cgcaaagcgg aaatcgaggt gcgccagggc
     5641 acaggccacc gcgatgccgc cgagctgaaa gctgccgagc aaatcgccgc actgcccctc
     5701 gaggtggtca agcgtggcgc tgatcttgcg cgcaaaactc gatagatgcg tcgccgattg
     5761 gccgccggtt gggcgattca tctcgccaag gcgcgcgatg ttgagctcca tcaacccgtc
     5821 gcccagcgcc tcgaggcgcc gcacgcgcca gcgcgcggca ggctcggtcg gcagcagggg
     5881 aggcccggcg tgcagagtgt cgaagaactc gcaaatgacc cgtgagtcaa atagcgcagt
     5941 gccgtcggcc aacagcagcg tcgggatctt gttgagggga ttggccgcca tcacctcggc
     6001 gttgggcttg ttggccgcga ccaccacgcg ggtaacgctc aggcgctcgg caagcccgct
     6061 ttcgtgggcc gcgaccagca ccttgcgcac gaagggggag cgcgaggagc cgaacagcgt
     6121 catcgacgtg tgtgcctgat ccatgcccct cccctcccgt gccaagtcac agcttttaac
     6181 ttccgcgcca aggcgcaatt ctaaccggcg ggcgcgggca atcaaaccct ggttgctgcc
     6241 ggcccaacac aagggcataa cggaggctca gtcctcgcgc caggaaagtc gccgcatgcc
     6301 cgcacccgcg ccctcacccc ttgccacgta tcttgcccat ctcgagcgcg gtcagctcgc
     6361 ctatcagttc agcccgcagg cgggccgcgc ggttttttat ccgcgtctcg tctgcccatt
     6421 cacgggcagc gaccagctgg aatggcgcgt cagcaagggc ttgggcacgg tctactccac
     6481 caccgtcgtg catccgcaag agggcgcccc ctacaacgtg gcgctgatcg actgcgacga
     6541 gggcttccgg ctgatgagcc gggtcgaggg cgtcgccccg caacaggttc gcatcggcat
     6601 gcgcgttgaa tttcgcgccc atatccccgg cggcgaagcg ccgccgatgc cggttttcgt
     6661 tgcggtggag gcctgagcca tgcagggtct aaagcgcgcc agcgccgcca tcgtcggtgt
     6721 tgcggaatcc gatctgggcc aggtcgccga tggcctatcg gtgatcgacc tcatggcaca
     6781 gggcaccaag cgtgcgctcg acgattgcgg gctcgcgctc agggatgtcg atggcttgtt
     6841 ctctgctacg gcgcagagcc gcctgtcggt gctggcgttc gcggaatatc tgggcatcga
     6901 tccgccctgc ctcggctcca ccatcgtcgg cgggtcgtcc ttcgagtacc atgtcgccca
     6961 cgccatggcc gcgattgagc agggcttgtg cagcgtcgcg gtgatcgcct acggcagcac
     7021 gcagcgcacg atcggtcgca agcaagcctc ccagcgcgaa atcaacccct atgagacgcc
     7081 cttcaagccc ttcctgcccg ccagtgccta cgcgcttgcc gcgatgcgcc acatgcacca
     7141 attcggcacc acgcgcgaac agctcgccgc cgtcgccgtg gcggcccgcg agtgggcgct
     7201 gctcaatccc gcggcctggg aaaagaaacc cttgagcatc gagggcgtgc tctcctcgcg
     7261 catggtgagc tacccattta cggtacgcga ctgctgtctc gtgaccgacg gcggcggcgc
     7321 catcgtggtg acttcggcgg aacgcgcccg ggcgctgaag aagccgccgg cctatgtgct
     7381 ggggtgcggc caatccgtga cccacgcaag catctccaac atgcccgacc tgaccgttac
     7441 gggggctgtc gcatcgggcc aggccgccta ccggatggcc aagcttgggc ccgccgacgt
     7501 cgacgtgctc gagctctacg acgcctttac cctcaacacc gtgctcttcc tcgaggatct
     7561 cggcttctgc aagaagggcg agggaggcgc attcgtcagc gaggggcgca tcgcccccaa
     7621 gggcagcctt gccgtcaaca ccaatggcgg cggcctttcc tactgccacc ccggcatgta
     7681 cggcctgttt ttgctgatcg aagcggtgcg gcaattgcgc ggcgaatgcg gggcgcggca
     7741 ggtcgccgat tgcgaggtcg ccattgcgca tggcaacggc ggggtgttgt cttcacaggc
     7801 gaccgtcatc ctcggtacgc cggcgacgct ctaatccctc aagaagcgca ttatcgacag
     7861 gtggtgacaa aggcgcacgg cttttgccca tccgcataga agtcgtaacg gaacccggcg
     7921 gtaaagaaca ggtcggtttc gttttgcgtc gccccggcct gctgccgttc cgtccacttg
     7981 gcgccgatct ccagctccaa actcaggtct cgggtccagt agtagttgat caggatggaa
     8041 ggcagcaccg tgatttcgcg caagtcggtg gtggcaccca cctggtagcc gaggcgcaag
     8101 cgggggttga tccgcagttc gttgctgagg ggatagcgcg cgctgaggtc tagaacatag
     8161 aggttggagg cctccaagtc ggccatgcgc acgccggcga tatacatatc gccctcggca
     8221 aacaagttcg ttcccgtcag ttgggcggag taatagagct cggtgcccgt cggcagcatg
     8281 gcctcgatcc cgcccgaggc aacggtgccg gaaaggctcg acgccgtggc atcgaggttt
     8341 acctgataat tcttgccgag cggaaaggca tagcccagcg tcgccgattt gaaggtgggc
     8401 gtacgatcga gcgcgaactg gtccacctca tccttgctgt gcagcttcag catgtcatag
     8461 agggtgagga agggctggcc ttgcagggcg ttccaagccg tcagataggg tgacttgcgg
     8521 tagtcgatgc cggcgctgat ggtggacttg tcgggcagcg tccaggagcc gctgaacatc
     8581 gccgcattga gctcctggaa atgcacgtcg tagtcgagga gcgcaaaggc agacttattc
     8641 gcatcgatat agcgcagctc cccgccgacc gcctgccgat cgaggatcga gcggtcgcgt
     8701 tgctcgatgg caaacagcgt cgcctccaag ttgccccaga aatggccgaa gtcgacgctg
     8761 gctccgtaaa agtagcgctc gtccttgaag ggctcgtcgg cccgccgctc gaccggggag
     8821 ccgcccacca cgttgacgcg cacgttggga tttgcctgcc aactgacgac gccgccatca
     8881 aagcggccaa ccacgccgcc cgtattgcgg gtttgccgac cgagccgggt catgaggtcc
     8941 cactgcttga tggtcgtttc gaagaacagg gcggcgacgc tgacaatctc cctgttgtcg
     9001 tcgaacttgt gctcctccgt gccggagaag cggaacttgg acttgaactg gtcgttgctc
     9061 caaacggcgc tcagatcgaa gctggagagc agcgagtttt ggtgtgtgag atgggcgtcc
     9121 ttgtcttcgt tggggttcgg cggtagcgag ggatcgcgca gcgtacgaaa gctgtcgtca
     9181 cgaatgtaga attgcgaggc gctgcccgac accatccagg tcgtgccgct gcgcggcggc
     9241 cctccgggct cgccgggctg cgccttgccg gagcgcaacc gctcgcccgg ctcgctgctg
     9301 gcggtgagaa tgccgtcgag acgctggcgt acgccctcgc ttccctcggc attggggtag
     9361 cggctgagat agtcctcata ttgcgcgcgc gcctcgccaa tgtggccggc cttttggtag
     9421 gcgatgccga ggagttcctg ggcctcggcg ctgcgctcgt tttccggccg cgtgagaacc
     9481 ttgctcaaga gctcgatggc ttgcgcgtaa ttgcccttct tcagggcggc cctcgcctca
     9541 tccatcgccg cggcaacggc gctgagttcg gcgggcgcga gtgcgccggc ggcgcgccct
     9601 tttggccgca ccggcggtgg cggcagcggc ccgcctggtt tggcagggtt gaggttgggt
     9661 gcccaatccc catgcgacac cgtcgtgtcc cagccggcgc cgaggcgtga gggaaagcgc
     9721 ggctgacagg ccgcctgcgg ggtcttgccg gcgatggcaa tcaccaggct ttcgaagtcg
     9781 gcgccggggg cgacctggta ggcaacgggg tgcaggaact gcacgcgcag gacgggtccg
     9841 gcgggctgat cgagttcgaa ctcgatggcg cggatggcgg cggcggcact ggccggaggg
     9901 cgcagggcct cccggcgcag gatggcgagc gcggccgctt gggtcgggtc gatggcgcgc
     9961 acgttgatgc gcaattcctc gcccgtcgcg agcgggaaat ggctgacgta gcggatgcgg
    10021 aaattgaact cgattttgac gagggtgcag gtcggcgagg gcgagaacag ccgcagtccc
    10081 gacagggcgc ggtcgagcac cggctcggcg gctgcggggt tgagcggcgc gcagctgaca
    10141 atggcggcgg ccgcgacgag gagcgggcgc aaagcccgag cccatgccaa acaccctcgc
    10201 ggcgacctaa cgcccaacag catcactact cacgtcctac tcgttactca caaagaacga
    10261 acgaattcaa tcgccgaagc tgcgtgtgtt gcggtgacag ttttcacagg gcgccgtcgt
    10321 attggggtgt ccgctgcgcg gcttgccgat ggcatagacg ccgttgtggc aagtggcgca
    10381 gccgctgacg atgcccgtgt ggttcatcct ggtgccggcg aatgacaccg tgctgcggtg
    10441 gcaggtctcg cagggctggg tggtggggat gtgggtggcg ggcttgccga gcgccgtggt
    10501 gccgttgtgg cagccggcgc agccggtggt gatgccggtg tggttcatga cggcgccgac
    10561 gaatgacacg gtgctcttgt gacaggtctc gcaggccgcc gtggtgggca cgtgcttggc
    10621 gggcttgccg agcgcggtgg tgccgttgtg gcagccggca cagccgctgc tgatgccggt
    10681 atggttcatg gcggcgccgg caaaggagac cgtcgatttg tggcaggtct cgcaggccgc
    10741 cgaggtcgcc acgtgcttgg cgggcttgcc caaggccatg gtgccgttgt ggcaggtcgc
    10801 gcagccactg ctgatgccgg catggttcat ggccgtgccg acgaagctga ccgtcgactt
    10861 gtggcaggtt tcgcaggcgg ccgtggtggg gatgtgcttg gccgatttgc cgagtgcggt
    10921 ggtgccgttg tggcagctgg cgcagccggt cgtcacgttg gtgtggacga actgggcgcc
    10981 agcaaacgaa accgtgctct tgtggcacat ttcgcaggag gccgaggtcg cgacgtgggt
    11041 cgccggtttg ccgagcgcca tgctgccgtt atggcagctg gcgcaacccg tggtgatacc
    11101 ggtgtggttc atggccgtgc cgacgaagct ggccgtcgac ttgtggcagg tttcgcaggc
    11161 agcgctggtc ggaatgtgct tggcggattt gcccacggcg ctgctaccgt tgtggcaagt
    11221 cgcgcagcca ctgctgatgc cgctgtggtt catggccgtg ccggcgaacg agacggtgct
    11281 cttgtggcag gtttcgcagg cggccgtggt ggggatgtgc ttggccgact tgccgagtgc
    11341 ggtggtgccg ttgtggcagc tggcgcagcc ggtcgtcacg ttggtgtgga cgaactgagc
    11401 gccggcaaac gaggtcgtgc tcttgtgaca ggtttcgcaa ggggccgagg tgacgatgtg
    11461 ggtggcgggc ttgccgagcg cggtggcgcc gttgtggcag gaggcgcagt tgctgacgcc
    11521 gacagggtgg tcaaagcggg caccggcgaa cgaggtcgtg ctcttgtggc aggtctcgca
    11581 gggcgccgac gtggcaatat gcgacttcga tttgcccagt gccttgctgc cgttgtgaca
    11641 ggaggcgcag tttcccgtaa tgccattgtg atcccaacgc gcggcgagga acgagaaggt
    11701 gcttttgtgg caggtctcgc aggcggcggt ggtgtcgatg tgggttggcg gtttggcgcg
    11761 cgcatggctg ccgttgtggc agctggcgca ggcgccgatg atttggttgt gattgaagcg
    11821 gacctgcgcc caggtcgccg tggtatggca ggcctcgcag ttggccgtgg tcggtccgtg
    11881 tgtgctgcgc tgaccggggg cgatgctgcc gttgtggcag ccgatgcact gccggggcgt
    11941 gcccttgaaa cggccgttga cgtggcacga ggtgcagtcg acgctgtagt gactgcccag
    12001 cagcggaaag ccggtcgtga agtggtcgaa ggcgcgggtc tgggcctcgg cgcgcgtacc
    12061 caacagctcg gccccggcca tgccgaagca gagcaggatc gcaattgccc tcagcagcgc
    12121 attcatccgt tcacagccta tacactccgt tcttttgtga tggttgtttg cccgcgattt
    12181 ggcccggtgg gccggatcgg cgatttcagc ctagccgcta gcgaggccgg accagcaagg
    12241 tcgcgaccgg cggcgagcat cgtcgaggag gtgctggaat aacggcaatc cgccctgcac
    12301 ggtcgcccgt cgaccgtcag acccctgccc aatttgccga ccgttccgtc gtccgtaggc
    12361 accctagttc ggagcgcacg gttctgcacg cgcaccaaac ataacttgca accacaacgc
    12421 gccctagtgg cgactattcc gtccgaggtc tgaaaagttt cgctttttac cgcaggcgaa
    12481 cgatcggagt gcggatgaga tgggtttctg gcatggccgc ggcgtggtgg cggacattgg
    12541 gctcaccgcc gccgcctcgt gcgcccgaca tctcgcgacg taagggggcg cagtcccagc
    12601 ggcgcgcgct gtgatggtgc aaacgccagt ggcggtggat gccaaagcaa acggcccgcc
    12661 ggcggtcaga gccgtcgcgg cgggccggtc ccccaagttc ccgtctgctc gctcgtcgcg
    12721 ggcgtgtcgc ttagcgcacc caacccgcga cgattgccgc cttttcctcg gcgtccggat
    12781 aaaccttgcc gccgctggcg acgcggatgg cggcctcgag cgccgcgttg gcggcatcaa
    12841 cgttgggtcc cgaagccggc gcgtaacccg ggcccgggtt ggcgggcatc gcttgcagct
    12901 tgagatgcgc ctgcacggcc cactggccgt ccttgccgcg gcaggcgaaa ccagcaaagt
    12961 gctcctgtgc cgtgctcctg agctcatagc gacggcaggg ttgctggtcg ccggagcggc
    13021 ggaaggtgaa tgccgtctcg agctcccacg tgccgcctgc ggccagctcg gctctgacgg
    13081 ggatgccgcg ggcggtatgc tcgagcagct cctgcagggg accttgcgcc gtcagctgac
    13141 cgtcgctgcc cccgatgaag cctgccgtcg gcacggacga ggtggccctg ggctgcagca
    13201 gcgagcccaa ggcggcgccg atcaacaagg ccagcgaagc ggccagcgcc atgggatagt
    13261 gctggtgcca gggatgccgg ggcgcgcgcg tgggccgcgc ttgcggcagc tggcgcactt
    13321 cggcggtacg cggggacgtc tgaccggctg ccaggtcggc acggcggatc tgatcgatca
    13381 ggggcccgag gtggtcgatc cggatcgtct cgcggaagag attttgtagt ggagacagcg
    13441 tggtacggaa tttttcgacc ttctgtcggc actccgggtg cctttgcaac agcgcctcga
    13501 cggcgtcccg ctcggcgggg tcgagggcac catcggcaaa cgccatcaac acttcatctg
    13561 acactacgga cacgacgagt ctccattgtc gtggtcgcct tggcaggcgc cccattgatt
    13621 gcatcgtgga tcgccagacg cgcacgagcc aaccggctca tgaccgtacc cacggggacc
    13681 ttcagaactt cggctgcctc ctgataggtt agcccgtcca cacaaaccaa agcgatcagc
    13741 acccgatgct caggcgagag ttgctcgagg ccacgaagca ccgccgcgag ctccaacctg
    13801 ttttccacaa ccgcgcgtcc gtcgcttccg accaacgtat cgaccgcgtc gacgtcgatg
    13861 gattcgccgc ggaaccgatc ggcccgcttg cgatcgagcc ataggttttg cgcgatgcga
    13921 aacatccaac tgtcgagccg cgtcccgggc tgccatttgt cccgattggc caaagcacgg
    13981 gcacacgtct cctgcacgag gtcgtcacgc tgatctgcat ttcccgttag ggcatatgca
    14041 aacccggcaa ggcgcggcaa gaaggcgatc attctctcgc gaaaatcgtc gccgacggct
    14101 tcaccttgcg ggcctgccac ataacgcgcg ggagggcccc ctattccctg gcggggtgtg
    14161 ccccgattac ccgccgcggc aagcgaagct tttgagcccg tggtcaattt cattgcctgc
    14221 tcaatccctg cctgaaggaa acggtggtgt gacatttttc gcacgcccgg ccaaaagctc
    14281 cgtcatgcac gtcgtccttg ccatggcagg cgtaacaagt atcgggggcg ctgaccttgg
    14341 tgaccgcgcg cgtggtgtgg caaccatgac actgcagcgc gcggtgggcg ccggtcaggg
    14401 catagcgggt ctgcttgtcg tggtcgaaac gccagcgcga ccagccgttc gggttgtggc
    14461 aggtcgcgca attggggccg agccggccgg catggtgcgc gtcggtgtgg cagccggcac
    14521 aagcgcgcga ggcctccttg aaggaggggg ttcgatggca ggcctcgcag gcgaccgcgg
    14581 catgcaggcc gatcagggga aaccgcgtca ggtcgtggtc aaaggtgacc gcctggcgcc
    14641 agccggtttc cttgtggcag gtctcgcagg cggccccgag ctggcccttg tgggggtcat
    14701 ccttgcggtg gcagccggcg caggtggtgg ccaacttgtc acgccggggc tcgccgctgt
    14761 ggcagcgttc acaggcaagc ttggcatggg cgccgcgcaa ggggaaccgc gtcgccttgt
    14821 tgtggtcgaa ggtcacggcg gcccattttg tcggggtatg gcagctctcg caggcactgc
    14881 cattgcggcc cgcgtgcttg tcctgcaggc cgtgacagga gctgcaggtg gtggccaagc
    14941 cctgatagcg ctcgccggcg tggcatttgc cgcaggcgac ctgtttgtgg gcggcaagga
    15001 gcgcaaacct ggtcttgctg tgatcgaagg gtttcacgcg cctccaggcc tcctcgccgt
    15061 ggcagccatc gcacttttcc cccaaccggc ctttgtgcgg atcgatggac ctgtggcagt
    15121 cgacacagcg cccgggcgtc tcgcggtatt tcgtgttctt tacatggcag ccgtcgcaac
    15181 ttgcgcgttt gtgagaccct gtcagggtga aattggcaaa agcgtggttg aaggtttcgc
    15241 ggttgagttg aacgatgtcg aacgtgcgcc ccttgtgctc ggtgtgacag gccttgcact
    15301 cgctgccgag ggcttccggt tggcggccgt ggtagttgcg cttggcctga cgatccgccg
    15361 cgatcggctt gtggcaggcc aaacagagcc cggactgcga tttttgcgcg aacggctcat
    15421 ggcagctttt gcaggtcttc tccagctccg catgggcgcc aatcaacggt cccggcatga
    15481 ccagcttttc gaagatgccc tgtgcacgtg ccatggatag ggtcgccgtc agcagcaaaa
    15541 cacccaataa gatcaatgtc ccgtgccgtc gcaccggcgt tccgatcagt agagatgcac
    15601 ggcaaccacg tggatgacgg ccgcgaacag cagcagcgcg aacagcggca ggtgcaggac
    15661 atgccacaag gcaaataggc gctcgtagaa gacgaaagcg gcagtcttgt tgaccgcggc
    15721 aaggtagtcc tcgatcagcc tgcgcgcggc cttgccatgt cgacgcctga ggcgccgcga
    15781 ccagccgagc cgcttggctt ccgcgccgac atgccgtgcg gccaactggc tgagccgggc
    15841 cttgaggccg agcgaggcta agcgcagggc cagaaaggca aaaagcgacg tgagcgcacc
    15901 gcgccggcga cgcgcgcgct cggcgtgtcg atcgagcgca gccagaacat cggccggcat
    15961 cgacagctcg ccttcgatcg cgtccttgag cgcggcgacg tcggcttgca actcggcgat
    16021 ttcagcgcga cggccgtaaa ggccgaggtg gatgcggcca tagaggtagc gaccaatgag
    16081 gccactgccg gccaccgtca gcattgccaa cagggcaacg ttgctgttga gggagccgag
    16141 cttgaaattc gcatgcagca gaatgagcgc cggaccgatc acgccgagca gcatgtgcag
    16201 ccggaaccag ccactgacgc tgcccaagag ttgcaagccc gccaggcgtt tgcgcagggg
    16261 atagcccaag agaagaagca tggcgctggc gccgaggatg ccgagccagt agccgaggcc
    16321 agcctccggc gtcaggtggc cctcatcgcg catctgccag ccataaatga gcgctgccgc
    16381 caggaggctt gcaaagccga gcgaggcgag gcggctgccg agcgtgggct caggggccgc
    16441 ggtcttgggc ggcgcggggc gcggcgcggc gggctcggct ttgccggccg ccgcggccgg
    16501 ttttggcgcc cgggccgtcg attttgctgc ggggcttggc gccgtcgggg cggcttccgc
    16561 gcgccgccgc atcggtaagg aggctgaagc cgtcatcgtc cccccgcgcg cgccaagcgc
    16621 atgtttctcc ccccaggagg cggtccggag gcagccgcat ccggtggctc gctcgataat
    16681 ggcgcccaat cgagacccca actctacttg tcacaaaccg gcgcttttgt ggccgaacct
    16741 tggtgaaggc accgcggcgc gtgcgccgcg ccggtgcctt gttgttaaag gcgtgcatgc
    16801 tgccggcgtt ggcgcacgcg ctcctcccta atttcgcgcc aatgcatggg aatagtcgct
    16861 tagcaacaag cgttgtgccg acatatcggc gcgccgcgac gcaacggatc gatgcgcctg
    16921 ctgatgaaat cgctcacaac aaagaccatc gctaaaaaga cgatggcatt aactatagcg
    16981 ccgcattttt cggagccggc acgatgtcgc cctcgctggt aatgaccctc gctctggttg
    17041 gcggcactgt tgccgcgctc gtcgttgtcg tcgctcgcgc cgcggccagt ccgagcagcg
    17101 agctctcgca tgcgctcgcc caactcggcg tcgttcgctt gaaggagctc gagcgtacct
    17161 ggcgcggcag aacggatgag gcccgcatcg aactgctccg ccaactgctg gaagaggcgc
    17221 gttcctcccg agcttggcgg tggccaagcc gcggcaagcc gacggtgctc gtcgcgggaa
    17281 gcctgctcgc acttctgttg tgtagcctca cgctgttggg ctcggccgat gcgccgttgg
    17341 cgacgccgcg ggtacccgaa ggcacgggct ggctgtcgca agatcccgac gtggcgcgcc
    17401 tggagctcta tgccaaaacc aaagccccgc gcccggcagc gagtgccgtg gccgccaaaa
    17461 cggcggagct gcccgacgtt gacaccatga tcgagcgctt ggccctgcgc ttgcaaaaga
    17521 agcctgacga tgccgagggc tggcgcatgc tgggttggtc gtttttcaac gtgcagcagc
    17581 cggccaaagc ggtggaagcc tatgcgcgcg cggtcgcctt agagccgcag ggggcggcgc
    17641 ttaagagcgc ctacggcgag gcgatggtgg caaccgagaa cggcacggtg acgccgccgg
    17701 cgctcgaaac cttcaatgcg gcgctggcgc tcgagccgaa caatgccaag gcgcgctact
    17761 tcgtggcgct ggcgctggag cagagcggca agaagaaaga ggcgctcgat cgctggctcg
    17821 gcttgctggc ggagccaagc gacaacgaac cttgggccgg cgagttgcgg caacgcgcgc
    17881 cagccctggc gcgcgagctt ggcgtcagtc tcgcggtccc ggcgctcgcc gcggccacaa
    17941 caagcgacgg cgcgaccccc ggcgccgcta agccgcgggc cttgacggcg gcagagatgc
    18001 gcctcgcgca ggcgctcccg gccgagcagc gccaggccat gatccgcagc atggtgagcg
    18061 gcttggcgga tcggctcgag agcacgcccc gagacgaaga gggttggttg cggctgatcc
    18121 actcgcgcct tcaactcggc gaggacgacg ccgcgcgcgc ggcactcgca cgcgcgctgg
    18181 ccgtgttcgc cgacgatgcc tccgccggcg gccgcatcac cgccgccgcc aaggagctgg
    18241 gtctggccaa caactaactg tggcgtgagg actgggtagg acgtgccgct cgcattcgac
    18301 cccatcttgg cgttctacgc tgttccgctc gttgtgcttt ggacggtcta tgtcggcgtg
    18361 cgcaagaagg tcgacgcgcg cagccgctcg acccaggcgg aggcgcagga agcgggactg
    18421 acggagcccg cctcgctgca ccccctgatc gatcccgtgg catgcttggg ctgcggcgcc
    18481 tgcgttcgcg cctgtccgga ggttgccaac ggacaccctg tgctcggcat gatcgacgac
    18541 cgggccgagc tgatcgagcc cgcgagctgc atcgggcatg gagcctgcaa aaccgcctgt
    18601 ccgacggggg cgatcacgtt ggtgttcggc acggaaaccc gtggcgtcga gctgccgatc
    18661 gtcaaccccg acttccagac caatgtgccc gggctgttca tagccggcga actcggcggc
    18721 atgggcttga tcaagaatg
//
LOCUS       DASTMT010000449         5978 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_4189636, whole genome shotgun sequence.
ACCESSION   DASTMT010000449 DASTMT010000000
VERSION     DASTMT010000449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 5978)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5978)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5978
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4189636"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..547
                     /locus_tag="VFR00_10505"
     CDS             <1..547
                     /locus_tag="VFR00_10505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002711025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0159737.1"
                     /translation="WYAVQVHQTKYLVEIGFSPSVAAWALGLVSLAGIPGQIALGHLS
                     DRIGREWAWAAGGLGFAICYLALIAMQLAPNPLLIYVMIAAQGVLGYGLTSVLGAIVL
                     EIFQGRHYGSIFGTLMLAALAGGAAGPWLTGVLYDARGNYTLAFGIGAVISGLSAFAI
                     WRAAPRKVRMVAGRLPRRALP"
     gene            complement(589..1584)
                     /locus_tag="VFR00_10510"
     CDS             complement(589..1584)
                     /locus_tag="VFR00_10510"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015685179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003960 - NADPH:quinone reductase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase family protein"
                     /protein_id="HEU0159738.1"
                     /translation="MSEPGGYQAVVCEALGPIEGLRLQRLARVPLRPNSVRIAVAAAG
                     LNFPDVLMIRGTYQHKPALPFVPGLEVAGVVSEIADGIVTPAPGDSVMVALAGGGYAE
                     EAVAPLEQVWPMPAQFSFAQAATFRVAHLTAYHALHTRAGLQAGQTLLVLGAAGGVGL
                     ASVQIGKVLGARVIAAASNRDKLQAAERMGADLLIDSSSEPIGAAVKRETHGAGADVV
                     LDPIGLAQEEALRAVAWNGKLLVVGFTAGAIPAYQANRILLKGVSVLGVRAGEAGRRD
                     PALRQQEYAALARMAKTGQAKPFLSASFPLARFAEAMRLLAERRAIGRIALTMER"
     gene            complement(1585..3165)
                     /locus_tag="VFR00_10515"
     CDS             complement(1585..3165)
                     /locus_tag="VFR00_10515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013912826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0159739.1"
                     /translation="MLRTLPLLAATIAAVVVAPSGSGAQTPKRGGILNFAVVAETSGY
                     DCHASQTFALLHPLSPQYSLLVKWDATENSKIIGDLAKSWSIAPDGLTYTFSLHEGVR
                     FHDGSPLTSADIKASFERIITPPEGVVSVRKERFADVAAVETPDPATVVFRLKAVNAS
                     FLALLASPFNCIYSAQKLKQNPRYPDTEVMGSGPYRLVEHVRGSHWTAKRNEDYFRKG
                     LPYLDGYKAFFVKNTAVVPGILGGQFDAEFRGQNPAERDQLLARGKDRLVAYEGPWAT
                     VMLLIFNTEKKPFDDVRVRQALSLALDRWAGGANLSKISIMKHVGGIMRPGSEWALPE
                     AELEQQPGYARDVEKSRAQARELLKKAGVENLTIKLFNRTVSEPYTPAGIFVINEWRK
                     IGVGAEHVQVETKTYFDSLVAGTFEVAIWPLTEPADDPSAQLYGFITNKASTLSYSRH
                     NDSKLDELFERQQRTLDPALRLQLVREADRYALTQAYSVPILWWNRIVVHDKKIKGWT
                     MSPSHFQGTDLLNVWLDQ"
     gene            complement(3257..4261)
                     /locus_tag="VFR00_10520"
     CDS             complement(3257..4261)
                     /locus_tag="VFR00_10520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0159740.1"
                     /translation="MRVLLSFVLLWCLCGSAFTARAETEVIRIPRGAGGVGFLPLLIM
                     EQRGLVERQARALGAANLKVDWIKLGGPAVVNDLLLSGAADIVVAGPPAFLTLWDRTR
                     ETLKVKGVAAMNSIPMYLNTRAGHLHSLKDLSANDKIAVTAVKVSIPAIIMQMAALKE
                     FGPAGYAHYDPFTVSLTHADAVIALTSGRADITAHFASPPFHQRELKLPGVRTIMSSN
                     AVMGGPSTFTLLYTTSRFHDANPKAFAAFSKALEEAILSLNADKRAAAQTFIDMEAQA
                     MPLAEVMEVLADPDIRYTTTPENIMKYADFMATVGSLKGRPSTWQDLFFPGIHHVPGG
                     "
     gene            complement(4291..5247)
                     /locus_tag="VFR00_10525"
     CDS             complement(4291..5247)
                     /locus_tag="VFR00_10525"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007825113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU0159741.1"
                     /translation="MQIGFIGLGLMGASMAANLQKAGYRLIVNDVRRTAAEPHLGRGA
                     SWGETPRAVAAVSDVVFTSLPGPPEVEAVAIGENGLIAGLRKETAYFDLSTNAPSLVR
                     KLHARFAIDGVHMLDAPVSGGPAGARSGRLALWVGGEEAVFQRHKALLDAMGDQARYV
                     GPIGAGSVAKLVHNCAGYAINAALAEVFALGVKGGVEPLALWEAVRTGAVGRRRTFDA
                     LIDQFLPGKYDPAHFALRLAHKDVSLATQLGRELSVPMRLAELALAEMTEALNRGWAG
                     RDSRVAMLPQLERSGVSIAVDAERIKAALERDPPFSADPKRS"
     gene            complement(5307..>5978)
                     /locus_tag="VFR00_10530"
     CDS             complement(5307..>5978)
                     /locus_tag="VFR00_10530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018320617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acyl homoserine lactonase family protein"
                     /protein_id="HEU0159742.1"
                     /translation="LIDTGFTAEMARARKRSFLRCPIDSLALLGVDAGAVRDVVLTHM
                     HYDHVGNFHKFANARFHLQEREMAYATGKYMRYPRIGHSYHVEDVVGMVRLNFKGRVE
                     MHDGEVELAPGLTLHPTYGHSDGLQSVRVNTKRGWVVLASDATHYYENLATNRPFSTA
                     FHVGQMLDAYRTLERLAPTPRHIVPGHDPYVMREYPAATPALEGIVVRLDAEPIAPAL
                     DFAPS"
BASE COUNT         1044 a         1913 c         1983 g         1038 t
ORIGIN      
        1 ctggtatgcc gtgcaagtgc accagaccaa gtatctggtg gaaatcggct ttagcccaag
       61 cgttgccgcc tgggcgctgg ggctcgtgag ccttgccggc atccccggcc aaatcgcgct
      121 tggccatttg tccgatcgca tcggcaggga atgggcctgg gcagcgggcg ggctcggctt
      181 tgccatttgc tatctcgccc tcatcgccat gcaattggcg ccgaacccgc tgctaatcta
      241 cgtgatgatc gcggcgcagg gggtcttggg ctacggcctg acctcggtgc tgggcgccat
      301 tgtgctggaa atctttcagg gccggcatta cggcagcatc ttcggtacct tgatgctggc
      361 cgcgctcgcc ggtggggccg cgggtccatg gctcacagga gtgctctacg atgcccgcgg
      421 caactacacg ctggcgtttg gcatcggtgc ggtcatcagc gggttgtcgg cgttcgccat
      481 ctggcgcgcg gcaccgcgca aggtgcgcat ggtcgcgggc cgcttgccgc gccgcgcgct
      541 gccctaacgg gatagcagcc cctgcggcag cccaggcccg cgacgatctt agcgctccat
      601 cgtcaaggca atgcgcccaa tcgcgcgtcg ctcggccaac aggcgcatag cctcggcaaa
      661 gcgcgcgagg ggaaagctcg cagacaagaa gggtttggcc tgcccggtct tggccatacg
      721 cgcgagcgcg gcatattcct gttggcgcag ggccggatcc ctgcgcccgg cctcgccggc
      781 gcgcacgccc aggacactca cgcctttcaa caggatgcgg ttggcttggt aggccggaat
      841 ggcgcccgcc gtgaagccaa ccacgagcaa cttgccgttc caggccacgg cccgcagcgc
      901 ctcctcctga gcaagaccga tgggatcgag cacgacatcg gcaccggcgc cgtgggtttc
      961 gcgcttgacg gcggcaccga tgggctcaga gctgctgtcg atcaaaagat cggcgcccat
     1021 gcgctcggcg gcctgcaact tatcccggtt ggaggcagcg gcaatcacgc gcgcgcccaa
     1081 caccttgccg atctgcaccg aggccaggcc aacgccgccg gcggcgccca gcacgaggag
     1141 tgtctggccg gcttgcagcc cggcgcgcgt gtggagggcg tgataagccg tcaagtgcgc
     1201 gacccggaaa gtcgccgctt gcgcgaacga aaattgagcc ggcattggcc acacctgctc
     1261 gagaggcgcg acggcctctt ccgcatagcc gccgccggcg agcgcgacca tgacactgtc
     1321 gcccggcgcc ggcgtgacaa tcccatcggc gatctcgctg acgaccccgg ccacttccag
     1381 acccggcaca aacggcagcg cgggcttatg ctggtaggta ccccggatca tcaatacgtc
     1441 ggggaagttg aggccggccg ccgccaccgc aatgcgcaca ctattggggc ggagtgggac
     1501 gcgcgcgagc cgctgcagcc gaagcccctc gatcggcccc agcgcctcgc agaccaccgc
     1561 ctggtagccg cccggttccg acacttactg gtcgagccac acgttgagga gatcggtgcc
     1621 ctggaaatga ctgggcgaca tggtccagcc cttgatcttc ttgtcgtgca cgacaatgcg
     1681 gttccaccac agaattggca cggaataggc ctgcgtcagg gcataccggt cggcttctcg
     1741 caccaattgc aagcgcagcg cgggatcgag cgtgcgctgc tggcgctcga acagctcatc
     1801 gagcttgcta tcgttgtgcc gcgagtaact cagcgtggag gccttgttgg tgatgaagcc
     1861 gtaaagttgc gcgctcggat catcggccgg ctcggtcagc ggccagatcg ccacctcgaa
     1921 ggttccggcc acgaggctgt cgaagtaggt tttggtctcg acctgcacat gttccgcgcc
     1981 aacgccgatc ttgcgccatt cgttgatcac gaaaatgccc gcgggcgtgt agggctccga
     2041 caccgtgcga ttgaagagct tgatggtgag gttctcaacc ccggctttct tcaggagctc
     2101 acgcgcctgc gcacgggatt tttcgacatc ccgcgcatag cctggctgct gctcgagctc
     2161 cgcctccggc aaggcccact ccgagcccgg gcgcatgatg ccgccgacgt gcttcatgat
     2221 ggagatcttg gacaagttgg cgccccccgc ccatcggtcc agtgccagcg agagggcctg
     2281 gcgcacgcgc acgtcgtcga acggtttctt ctccgtgttg aagatcagca gcatgaccgt
     2341 ggcccacggt ccctcgtagg cgaccagccg gtctttgcct ctggcgagga gctggtcgcg
     2401 ctcggccggg ttctgtccgc gaaattcggc atcgaactgg ccgccgagga tgcccggcac
     2461 cacggccgtg ttcttgacga agaacgcctt gtaaccgtcg aggtagggca ggcccttgcg
     2521 gaagtaatcc tcgttgcgtt tcgccgtcca gtgcgagccg cgcacgtgct cgacgaggcg
     2581 gtaggggccc gaccccatca cctcggtgtc ggggtatctc gggttctgct tgagcttctg
     2641 ggcgctgtag atgcagttga agggcgaggc cagcagcgcc aggaacgagg cgttgacggc
     2701 ctttagccgg aacaccacgg ttgccgggtc aggcgtctcg acggcggcga catcggcgaa
     2761 gcgctcctta cgcaccgaca ccacgccctc gggcggggtt atgatgcgct cgaagcttgc
     2821 cttgatatcg gcggaggtga gcggggaacc atcgtgaaac ctgacgccct cgtgcaggct
     2881 gaaggtatag gtcaggccat ccggcgcgat gctccagctc ttcgctaagt cgccgatgat
     2941 tttggaattt tccgttgcgt cccacttgac caacagcgag tactgcggac ttaaggggtg
     3001 cagcagcgca aaagtctggg aggcgtggca atcgtacccc gaggtctccg ccaccacggc
     3061 aaaattgaga atgccaccgc gcttgggcgt ttgcgccccg cttccgctcg gggccacgac
     3121 caccgcggca atggttgcag ccagtagcgg cagggtacgc agcatgccag tcctccctca
     3181 ttctttcttg tgcggcggga ggctacgaca ttgttccccg cgcccccaag tgaaaaggcg
     3241 cgcatgcccg gcgagcctag ccgccgggaa cgtgatgaat gcctgggaag aacaggtcct
     3301 gccaggtact cgggcgcccc ttgaggctgc cgaccgtcgc catgaagtcg gcatacttca
     3361 tgatgttttc gggcgtggtc gtgtagcgaa tgtcgggatc agccaggacc tccattacct
     3421 ccgccagcgg catggcctgc gcttccatgt caatgaaggt ctgcgccgcc gcgcgcttat
     3481 cggcgttgag gctcaaaatc gcctcctcca gggcctttga gaaggcggcg aaggctttgg
     3541 ggttggcgtc gtggaagcgg ctggtggtgt agagcagcgt gaatgtgctg ggcccgccca
     3601 tcaccgcatt ggagctcatg atcgtgcgaa ccccaggaag cttcagctcc cgctgatgga
     3661 acgggggaga ggcaaaatgc gccgtgatgt cggcgcgtcc ggaggtcaag gcgatcacgg
     3721 cgtccgcatg cgtgagggag accgtgaaag gatcatagtg ggcatagcct gccggcccga
     3781 actccttgag ggccgccatt tgcatgatga tcgcagggat cgaaaccttc acggcggtaa
     3841 cggcgatctt gtcgttggcg ctgagatcct tgagcgaatg gaggtgcccg gcgcgggtat
     3901 tgaggtacat ggggatcgag ttcatggccg cgacgccttt gaccttgagc gtctcacgcg
     3961 tgcgatccca gagggtcaga aaggcgggcg ggcccgcaac gacgatatcg gccgctccgg
     4021 acaggagcag gtcattcacc accgccggcc cgccaagctt gatccagtcg accttgagat
     4081 tggccgcccc cagggcgcgc gcctgccgtt caacgagacc cctctgctcc atgatgagaa
     4141 ggggcaagaa cccgacgccg ccggcgccac gcgggatgcg gatcacctcg gtctcggccc
     4201 tggcggtgaa ggcagagccg cagaggcacc agagcagcac gaaactgaga aggacgcgca
     4261 tgcacttgct tgctggtgag gcgccaggga tcagctgcgt ttggggtcgg cgctgaaggg
     4321 cgggtcgcgc tcgagcgcgg ccttgatgcg ttcggcgtcg accgcaatcg acaccccgga
     4381 gcgctcgagc tggggcagca tggccacgcg cgagtcgcgc cctgcccagc cacgattgag
     4441 cgcttccgtc atttccgcca gggcaagttc ggcaaggcgc atcggcacgc tgagctcgcg
     4501 gccgagctgg gtcgccagcg agacgtcctt gtgcgccaag cgcagcgcga agtgcgcggg
     4561 atcgtactta ccgggcagga attgatcgat cagggcatcg aaggtgcggc ggcggccaac
     4621 ggcacccgtg cgcaccgcct cccacagagc caatggctcg acaccgccct tgacgcccag
     4681 cgcaaacacc tccgccaggg ccgcgttgat ggcgtacccg gcgcagttgt gcacgagctt
     4741 ggccaccgag cccgcaccga tcggtccgac gtagcgcgcc tgatcgccca tggcatcgag
     4801 cagggccttg tggcgctgga agacggcctc ctcgccgccg acccacaggg caagacggcc
     4861 cgagcgggcg ccggcgggcc cgccgctgac gggggcatcg agcatatgca cgccatcaat
     4921 ggcgaagcgg gcatggagct tgcgcaccaa gctcggggcg ttggtcgaaa gatcgaagta
     4981 ggccgtctcc ttcctcaggc cggcgatgag gccattctcg ccaatggcaa ccgcctcgac
     5041 ctccggggga ccgggcagcg aggtgaagac cacgtcgctc accgcggcga cggcgcgcgg
     5101 ggtctctccc cagctcgccc cgcgccccaa gtgtggttcg gccgcggtgc gccgcacatc
     5161 gttgacgatc aggcgatagc ccgctttctg caggtttgcc gccatgctgg cacccataag
     5221 accgaggccg atgaatccaa tttgcatgtt gtgctccacg attgaccgac ggcgccgttg
     5281 gccggcgttt aggggcccga ggctactcaa gagggcgcaa agtcgagcgc gggtgcaatc
     5341 ggctcggcat cgagacgcac cacgatgcct tcgagtgcgg gcgtcgcggc cgggtattcg
     5401 cgcatgacat agggatcgtg acccggcacg atgtgtcgcg gcgtcggcgc tagtcgctcc
     5461 aaggtgcggt aagcatcgag catttgcccg acgtgaaagg ccgtgctgaa ggggcggttc
     5521 gtcgcgagat tttcatagta atgcgtggca tcggaagcaa gcaccaccca accgcgcttg
     5581 gtattcacgc gcacggactg gagcccgtcc gaatgtccat acgtgggatg cagcgtcagc
     5641 ccaggcgcca gctctacctc gccgtcgtgc atctcaactc gacccttgaa attgagtcgc
     5701 accatgccga ccacgtcctc gacgtgatag ctatgaccaa tgcgggggta gcgcatgtat
     5761 ttgcccgtag cgtaagccat ctcgcgttcc tgcagatgga agcgggcatt ggcaaatttg
     5821 tgaaagttgc cgacgtgatc gtagtgcatg tgggtcagca ccacgtcgcg aacggcgccg
     5881 gcgtccactc ccaacagcgc caggctgtcg atggggcagc gcaggaacga gcgcttgcgg
     5941 gcgcgcgcca tttccgcggt aaagccggtg tcgatcaa
//
LOCUS       DASTMT010000450         1798 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_2225928, whole genome shotgun sequence.
ACCESSION   DASTMT010000450 DASTMT010000000
VERSION     DASTMT010000450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 1798)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1798)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1798
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2225928"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(497..>1798)
                     /gene="der"
                     /locus_tag="VFR00_10535"
     CDS             complement(497..>1798)
                     /gene="der"
                     /locus_tag="VFR00_10535"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013419779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEU0159743.1"
                     /translation="LFNRLTGRRAALVSDTPGLTRDRRSGEATIAGNPVTVIDTAGLE
                     EGPSASIAGRMRTQSELALASADLVLFVFDARDGIMPADAVFARRVLASGVPAILVAN
                     KCEGRAGSEGFYEAFRLGLGEPIAISAEHGEGIGELNGAIAGALGLQPQGSLPAAADL
                     PREHSAVRIAIVGRPNAGKSTLVNALLGEDRMITGPEPGLTRDAVASDVTWQGRRVRL
                     FDTAGLRRKARVIERAEKLAVSDAVRAIRFAEVVVLLLDAEHPFEHQDLTIADLVESE
                     GRALVIAINKWDLVPNKQRMLKDLRATAAERLAQLPGVAIVPISALAGRGLDDLMSAV
                     FEAYAIWNKRVPTPELNRWLAQAVEQHAPAASKGRRVKLRFITQPSARPPTFITFCSR
                     PEALPKAYLRYLSNSLRAAFGFAGVPIRLKLRKSENPFAGG"
BASE COUNT          314 a          613 c          577 g          294 t
ORIGIN      
        1 cccgtagctt tgcgcaaaat cgcgcgttgg cggctgccgc ggggcgcaag ggcggccagg
       61 cctcccagag ccgtcggctg cgcagcagcg gcaatgggtc gagtgagcgg ggcggccacg
      121 gctcctccga taatccaggc cgcaccgact gagcgggcgc aagcccgccg tcttgcccac
      181 caaaggtgag ccgggcctgg gacgcgtgcg ccaggagcgc cacggcgcgg cccgttcgtc
      241 gcgcgcgcca ggccgggtgg gcttttttgc cgcgcccggc gacacctcat cgatcgccaa
      301 aagctttcgc cgatggtgag ccccgcccgt gagcctgcgt cgtgcctcgc ccaagcgcat
      361 ctgcccgcga cctagtctag gggccaggac cggtcacctg gacttagggc ccaccaaggc
      421 aatccacgcc agcgtcgatt aacctaatgg cgaagccggc ggtcgcgcgg tccgtcgctt
      481 gcggtcgacc tgagggtcaa ccgcccgcga agggattttc gctcttacgg agtttgagac
      541 ggatcggcac gcctgcaaag ccgaaggcgg cgcgcaggct attgctaaga tagcgcaggt
      601 aggcctttgg caaggcctcg ggccgcgagc agaaggtgat gaaggtcggc ggtctggccg
      661 aaggttgggt gataaatcgc agctttacgc ggcggccctt gctggccgcg ggggcatgct
      721 gttcaaccgc ttgggccagc cagcgattca actcgggcgt gggcacacgc ttgttccaaa
      781 tcgcataggc ctcaaacacg gccgacatca gatcgtcgag accgcggccc gccagcgccg
      841 agatcggcac gatggcgacg cccggcagtt gcgccagtcg ctcggccgcc gtggcgcgca
      901 gatctttcag cattctttgc ttattgggca ccaagtccca cttgttgatg gcgatgacga
      961 gggcgcggcc ttcggattcg accaggtcgg cgatagtcag atcctgatgt tcgaagggat
     1021 gctccgcatc aaggagcaac accaccactt cggcaaagcg tatggcacga acggcatcgc
     1081 tcaccgccag cttctcggcc ctctcgatga cacgcgcttt gcgcctcaga ccggcggtat
     1141 caaataagcg aacgcgccga ccttgccagg tgacgtcgct ggcgacggca tcacgcgtca
     1201 accccggctc agggccggta atcatgcggt cttcgccgag caaagcattg accagggtgg
     1261 atttaccggc gtttggccgc ccgacgatag cgatgcgcac cgcactatgt tcgcgcggta
     1321 agtcagctgc cgccggcagg ctgccttgcg gttgcaggcc cagcgcgccg gcgatcgcgc
     1381 cgttcaattc cccgattcct tccccatgct ccgccgaaat ggcaatcggc tcaccgagcc
     1441 ccaaccggaa tgcctcgtaa aagccctcag agcccgcgcg cccttcgcac ttattggcca
     1501 ccaaaatcgc cggcaccccc gacgccagaa cgcgtcgcgc aaacactgcg tcggccggca
     1561 tgatgccgtc ccgcgcatcg aacacaaata acaccaggtc ggcgctggcg agcgccaatt
     1621 cgctctgtgt ccgcatgcgc ccggcaatgg acgcgctcgg tccttcctcg agccccgccg
     1681 tatcgatcac ggtcacggga ttgccggcga ttgtcgcctc cccgctgcgg cgatcgcgcg
     1741 tcaggccggg cgtgtcggag acgagcgccg cacgcctgcc ggtcaagcgg ttgaagag
//
LOCUS       DASTMT010000451         1609 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_2546118, whole genome shotgun sequence.
ACCESSION   DASTMT010000451 DASTMT010000000
VERSION     DASTMT010000451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 1609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1609
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2546118"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            87..830
                     /gene="hisA"
                     /locus_tag="VFR00_10540"
     CDS             87..830
                     /gene="hisA"
                     /locus_tag="VFR00_10540"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006940207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HEU0159744.1"
                     /translation="MILFPAIDLKDGKCVRLKLGDMAAATVFNDDPAAQAKAFESQGF
                     RYLHLVDLDGAFAGRPVNAAAVASILATVRIPVQLGGGIRDLGTLETWLQAGVSRVIL
                     GTLAVRNPDLVRQAGRLYPGRIAVAIDAKDGTVAVEGWAVKSELSALDLARRCEDAGV
                     AAVIYTDVARDGVLKGLNLPATAALARATAIPVIASGGLAGIADVEALLRPEYAMLEG
                     AIAGRALYDGRLDARAALALLAAAGTGKT"
     gene            1306..>1609
                     /locus_tag="VFR00_10545"
     CDS             1306..>1609
                     /locus_tag="VFR00_10545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012383257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HEU0159745.1"
                     /translation="MLKVRIIPCLDVKDGRVVKGVNFQDLKDAGDPVEAAAAYDVAGA
                     DELCFLDIAASHEHRDTIFEIVERTASRCFMPLTVGGGVRTSGDIRRLLRAGADKVS"
BASE COUNT          234 a          553 c          527 g          295 t
ORIGIN      
        1 gactgtcaaa caacatcccc cactgatcgc cgatgaaagc agcaaactga tggctgcggc
       61 gcggcaggca ggtgcgagac cccgacgtga tcttgtttcc cgccatcgac ctcaaagacg
      121 gcaagtgcgt gcgcctgaag ctcggcgaca tggcggcggc caccgtgttc aacgacgatc
      181 ccgcggccca ggccaaggcc ttcgagagcc agggctttcg ttacctgcac ctggtcgacc
      241 tcgacggcgc cttcgccggc cggccggtca atgcggctgc ggttgcttcg atcctggcga
      301 ccgttcggat cccggtacaa ctcggtggcg gcatccgcga cctggggacg cttgaaacct
      361 ggctgcaggc cggtgtcagc cgcgtgatcc tcggcaccct cgccgttcgc aatcccgacc
      421 tggtccggca ggcgggacga ctttatcccg ggcgcatcgc cgttgccatc gatgccaaag
      481 acggcacggt cgccgtcgag ggttgggcgg tcaagagcga gctgtcggcc ctcgatttgg
      541 cgcgccgctg tgaggacgcc ggcgttgccg ccgtcattta caccgacgtt gcccgcgacg
      601 gcgtgctcaa gggtctcaat ttgcccgcta ccgccgcgct ggcgcgggca accgccattc
      661 ccgtcatcgc ctcgggcggt cttgccggca tcgccgacgt ggaggccttg ctgcgtcccg
      721 aatacgccat gctggaaggt gccatcgccg ggcgcgctct ctacgacggc cgcctcgacg
      781 cgcgtgcggc gctggctttg ctcgctgccg ccggcactgg caaaacctaa ggcctgcccc
      841 gctgcgtttt gccggtgcgg gtctgccttg agcgccatgg gcttgccacg gcgcttcgaa
      901 gctcgctcga cccggcattg gtgaaaaagg acccactggc cctgcgagcc gtcggcggac
      961 gttgcccgca gccaaatctg ccgctgccgc cggcgagggt ttgcggggca ctcgcagggt
     1021 ttgtcgcctg caagggggta gccgggggag gtcagcggga ctgggtctcg gggtctgccc
     1081 tgccctgtcg ctgtccgcca gacgaacggc cggtgctctg cccacacgcc gggtggcctc
     1141 gacgctgccg gccatcccgc accagaggcc gaggcccatt tctcgtttcg ccttgacggg
     1201 gcgccgcgca tcgccctcgc ctcgctaccg ccgccgcgct ccgctttttg ctgcgtcgac
     1261 tatttttggt tgcacgccat ccccgcccgt cgcacaaacc ccttcatgct caaagtccgg
     1321 atcattcctt gtctcgatgt caaagacggc cgcgtcgtca agggcgtcaa cttccaagac
     1381 ctcaaggatg cgggcgatcc cgtcgaggcg gccgcagcct acgatgtggc cggggcggac
     1441 gagttgtgct ttctcgatat tgcggcgagc cacgagcatc gcgacaccat tttcgagatc
     1501 gtggagcgta cggcctcgcg ctgcttcatg ccgctgacgg tggggggcgg cgtgcgcacc
     1561 agtggagaca ttcgcagatt gttgcgcgcg ggagccgata aggtttcca
//
LOCUS       DASTMT010000452         3539 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_5054743, whole genome shotgun sequence.
ACCESSION   DASTMT010000452 DASTMT010000000
VERSION     DASTMT010000452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3539
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5054743"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..271)
                     /locus_tag="VFR00_10550"
     CDS             complement(<1..271)
                     /locus_tag="VFR00_10550"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HEU0159746.1"
                     /translation="MNDQQASISQRLAERIASLDGAGVAPAVREVAERVLIDVIGLCV
                     AARHTAYVAAALAAWPEPGAATSIGHAAGMSAAAAAFINGTAAHGE"
     gene            complement(476..2200)
                     /locus_tag="VFR00_10555"
     CDS             complement(476..2200)
                     /locus_tag="VFR00_10555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016845494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEU0159747.1"
                     /translation="MTSDTLAASALPEVRARLDLSGAVMALFAGILALLIILPLAWLV
                     VFAFSDRARNPTLANFQLLFTDPAFLDPLLATFTIAFSVSAICCAIAAPMGWLVARTD
                     MPLRRPIRLLVMASFVTPPFLGAIAWELLAAPNSGLLNQLYRGVAGASADAKLFDIYS
                     LGGVIFVISCYTFPYVFVLVANALDRTPGELEDASSMLGASAWRTAQKITIPLALPAV
                     LAGALVAFLQAMTLFGSPAILALPAGFHTMTTKIWSLFQYPPKPELAAAASLPLLLMT
                     VILLRGQAALLGRRGYVVVGGKSGPPRPVRLGGLRWPALALCLAVLCLPVFLPYGALV
                     NAAFSRIASQPLSFETATLHNVHFVFFELSATKLAMRNTFLLGLMAATSGTVLALVIA
                     YLTARAAITGHRILAFLATAPIAIPGIVLGVGLFLAYTRGPVVLYGTLWILLLAYVTI
                     ELPAAYQQLQSAFRALSPELEEAGRILGASRLRTLKDVTAPLLGPSLVATWCFIFVAV
                     IRELSAAVILFTSETKVLSVLIFDLKESGDLGAIAVLGLTMLILTAGVIIAVNRIPGF
                     SGVRLRNN"
     gene            complement(2391..3452)
                     /locus_tag="VFR00_10560"
     CDS             complement(2391..3452)
                     /locus_tag="VFR00_10560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016845493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEU0159748.1"
                     /translation="MASAISRRQFSQGLVTQLAMASVAPAGWAAVPASSLTPQLVEAA
                     KKEGKAVWYTAVDLPVAERIAKAFEAKYPGIAVRVERSGGERIFQRIGQEYASNIHAV
                     DVVNSSDAAHFIVWKREGILAPYVPDEVASHYPAEQVDPDGLFASWRVWLCVIGYNTK
                     LVKPDEAPKSFADLLDPKWSGKIVKAHPGYSGTIMTATYQISRDIGWSYFEKLSRQKV
                     MQVQSSADPPKKLALGERAIMADGNEYNLIQLKEKGEPVEIVYPSEGTPLIIGPSGVM
                     KDAPNPNAARLLQSFMFSVECQQLMVDWGGLRSFHAQVKDKAGRKPLKNIKLMKDDPA
                     AVEKEADRIKAKYTQYFKV"
BASE COUNT          664 a         1115 c         1226 g          534 t
ORIGIN      
        1 cctcgccgtg cgctgccgtg ccattgatga aggccgcagc cgccgcgctc atgccggcgg
       61 cgtggccgat gctggtggcg gcgccgggtt ccggccaggc ggcgagcgcg gcggcgacat
      121 aggcggtgtg gcgcgcggca acgcacaagc cgatgacatc gatgagcacg cgctctgcga
      181 cctcgcgcac ggccggcgcc acgccggcgc catcgaggct ggcgatgcgc tcggccaacc
      241 gctgggaaat ggaggcttgc tgatcgttca cgtgacaccc gtcgctcgtt cgcaagagct
      301 gtcttagcca acggcggcgt ccggcgcacg cctgtcctta aaaaaaagcg ctccgccatt
      361 gccggacgcg cgagccgcgt cggggatggc cgcccccccg ggcggggagg aacagcgcgc
      421 gcctggaaaa attccggcgc gggagtgcct tgcgtcgcgg ccggccgcgc aggcgctagt
      481 tgttgcgcag tcgcaccccg ctgaagcccg ggatgcggtt gacggcgatg atgacgccgg
      541 cggtgaggat cagcatggtc aggccgagca cggcgatggc accgagatca ccgctctcct
      601 tcaagtcgaa gatcaacacc gacagcacct tggtctcgga ggtgaaaagg atcacggccg
      661 ccgacaactc gcggatcacg gcgacaaaaa tgaaacacca ggtagcaacc aaacttggcc
      721 cgagcagcgg cgccgtcacg tctttcagcg tgcgcaaccg tgaggccccg agaatgcgcc
      781 ccgcctcctc cagctcgggc gatagcgccc ggaaggcgga ttgcagttgt tggtaggccg
      841 ccggcaattc gatggtgaca taggccagca gcaggatcca cagcgtgccg tagagcacca
      901 ccggcccgcg cgtataggca agaaacagcc cgacgcccaa gacgatgccg ggaatggcaa
      961 tcggcgccgt ggcgaggaag gcgaggatcc ggtggccggt gatggcggcc cgcgcggtca
     1021 ggtaggcaat caccaaggcc agcaccgtgc ccgaggtcgc cgccatcagg ccgagcaaga
     1081 aggtgttgcg catggcgagc ttggtggccg agagctcgaa gaacacgaaa tgcacgttgt
     1141 gcaaggtggc ggtctcgaag ctgagcggct gcgaggcgat gcgcgagaag gcggcgttga
     1201 cgagcgcacc gtagggcaga aagaccggca ggcagagcac cgccaggcag agcgccagcg
     1261 cgggccagcg caggccgccg aggcgcacgg gtcgcggcgg cccgcttttg ccgccaacca
     1321 ctacgtagcc acgccggcca agcagcgccg cctggccgcg caacaggatc accgtcatca
     1381 gcaatagcgg caacgacgcc gcggcggcga gctccggctt gggcgggtac tggaacaggc
     1441 tccagatctt cgtcgtcatg gtgtgaaagc ccgccggcag cgccaagatc gccggcgagc
     1501 cgaacaaggt catggcttgc aggaaggcga cgagcgcacc cgccaacacg gcgggcagcg
     1561 ccaagggaat ggtaattttt tgcgccgtgc gccacgcgga cgccccgagc atggaagacg
     1621 cgtcctcgag ctcgccgggc gtccggtcga gcgcattggc gacgaggacg aacacgtaag
     1681 ggaacgtgta gcaggatatc acgaagatga cgccgcccag gctgtagatg tcgaacagct
     1741 tggcatcggc gctggcgccg gcgacgccgc ggtagagctg gttgaggagg ccgctgttgg
     1801 gggccgcaag cagctcccag gcaatggcgc cgagaaaagg gggcgtgacg aacgaggcca
     1861 tgaccagcag gcggatgggc cgtcgcaacg gcatgtcggt acgcgccacc agccagccca
     1921 tcggggcggc gatggcgcag caaatcgcgc tgaccgagaa cgcgattgta aaggtcgcga
     1981 gcagcggatc gaggaaggca gggtcggtga acagcagctg aaaattggcc agcgtcggat
     2041 tgcgcgcccg atcgctgaaa gcgaacacga cgagccaggc cagcggcaga atgatcaaga
     2101 gcgccaggat gccggcgaag agggccatca ccgcgcccga gagatcgagg cgggcgcgta
     2161 cctcgggcag cgccgaggcc gcaagcgtgt ccgatgtcat ggcgctaggc gtacccctcc
     2221 tcaacattcc ccgcgccaag tcgcgcgggc agtttgcggg cgatcccggc accaggcgag
     2281 gcgggccacc cgggagcctc gttgttgcgc cccatttggc ctagcacagg cctgacggcg
     2341 cagggcgaag ccatccgcga gcggcaccag gaccgccagt catcgcgcct ttagaccttg
     2401 aagtattgcg tatacttggc cttgatccga tcggcctctt tttccacggc agcgggatca
     2461 tccttcatca gcttgatgtt ctttaaaggc ttgcgtcccg ccttgtcctt gacctgcgcg
     2521 tggaaggagc gcaggccgcc ccagtcgacc atcaactgct ggcactcgac ggagaacatg
     2581 aagctttgca gcagccgggc cgcgttgggg ttgggcgcgt ctttcatgac acccgaggga
     2641 ccgatgatca gcggcgtgcc ctcgctggga tagacgatct cgaccggctc acccttttcc
     2701 ttgagctgga tcaggttgta ctcgttgcca tccgccatga tggcgcgttc gccgagcgcc
     2761 aacttcttcg gcggatcggc cgaggattgc acctgcatga ctttctgccg gctgagcttc
     2821 tcgaaatagg accagccgat gtcgcgggag atctgatagg tcgcggtcat gatggtgccg
     2881 ctgtagccgg gatgcgcctt gacgatcttg cccgaccact tcggatcgag cagatcggcg
     2941 aagctcttgg gggcctcatc cggcttgacg agcttggtgt tgtaaccgat cacgcacagc
     3001 catacgcgcc agctggcgaa caggccgtcc ggatcgacct gctcggccgg atagtggctc
     3061 gccacctcgt ccggcacgta aggggccagg atgccctcgc gtttccagac gatgaaatgg
     3121 gccgcatccg agctattgac gacgtcgacg gcgtggatgt tgctggcgta ttcctggccg
     3181 atccgctgaa aaatgcgctc gccgcccgag cgttcgacgc ggacggcaat gccgggatac
     3241 ttcgcctcga aggccttggc gatgcgttcg gcgaccggta ggtcgaccgc ggtgtaccag
     3301 accgctttgc cttccttctt ggcggcctcg accagctgcg gcgtgaggct cgaggcgggc
     3361 acggcagccc agcccgccgg cgccacggag gccatggcga gctgcgtgac gagcccctgg
     3421 gagaattggc gccggctgat cgcgctggcc atggtcttat cctttcgaaa tcggctgcct
     3481 tcaagtgatc aacgcgtggc agcggtcggc gggcaaaagc aaagtgacgc gtgcgcctg
//
LOCUS       DASTMT010000453         3295 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_589591, whole genome shotgun sequence.
ACCESSION   DASTMT010000453 DASTMT010000000
VERSION     DASTMT010000453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3295)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3295)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3295
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_589591"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(11..601)
                     /locus_tag="VFR00_10565"
     CDS             complement(11..601)
                     /locus_tag="VFR00_10565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0159749.1"
                     /translation="MQRLCLLFFAIAAAAAVGGCGSLPTLGGAPQASAPQPVASPPTA
                     AQTAAPQRTDLPRPGGQSAPFESAFIAEGNPTGLYALIARGALACWLGAGGPLNGTHV
                     FAAEAAPPTQGGTAEIVLHERDRDLRDQRGPRALRIRLERASGGVRVGFTNLRFDGPL
                     AERMARDVASWAKGGTGCGTEAPKPPPIVSAGAGRR"
     gene            complement(829..1206)
                     /locus_tag="VFR00_10570"
     CDS             complement(829..1206)
                     /locus_tag="VFR00_10570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009757980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HEU0159750.1"
                     /translation="MKPRDAALRLKRFEVSEKARTVVSMESMIGDFERMAHDLARQIA
                     AEEERTGIKDTSHFAYSTFAKAASLRRMNLLNSVADLRAKLDAARREHEEMATELSKL
                     EPVTTREGDRHDLSGERGGLMLN"
     gene            1867..2562
                     /locus_tag="VFR00_10575"
     CDS             1867..2562
                     /locus_tag="VFR00_10575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006203583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU0159751.1"
                     /translation="MRVLLIEDDSATAQSIELMLQSEGFNVYTTDLGEEGVDLGKLYD
                     YDIILLDLNLPDMSGYEVLKTLRVSKVQTPILILSGLAGIEDKVRGLGFGADDYMTKP
                     FHKDELVARIHAIVRRSKGHAQSVIETGELKVNLDTKTVEVHNQRVHLTSKEYQMLEL
                     LSLRKGTTLTKEMFLNHLYGGMDEPELKIIDVFICKLRKKLQAATGGQHYIETVWGRG
                     YVLRDPGDEHVAA"
     gene            2778..2993
                     /locus_tag="VFR00_10580"
     CDS             2778..2993
                     /locus_tag="VFR00_10580"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HEU0159752.1"
                     /translation="MSRQVATPVAGHLITCLVKVGDRVEVDTEVGIVEAMKMHIPVVA
                     EQSGRVAKWLVSENTTVAEGQPLIELV"
     gene            3111..>3295
                     /locus_tag="VFR00_10585"
     CDS             3111..>3295
                     /locus_tag="VFR00_10585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011157460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEU0159753.1"
                     /translation="MANSATLKVRVNRDKCQGHNRCKAIAPELFELDEFGNARAAGDG
                     RVPTGLEEKAYLAKSNCP"
BASE COUNT          598 a         1064 c         1052 g          581 t
ORIGIN      
        1 gcggcagggg ttagcggcgg ccggcgccgg ccgacacaat cggcggtggc ttgggcgcct
       61 cggtgccgca acccgttccc cctttggccc agcttgccac gtcgcgcgcc atccgttccg
      121 ccaacgggcc atcaaaacgc agattggtaa agccgacgcg cacgccgccc gaggcacgct
      181 ccaatcggat gcgcagcgcc cttgggcccc gctgatcacg caagtctcga tcgcgctcgt
      241 gcagcacgat ttcggccgta ccaccctggg tgggcggcgc ggcctcggcg gcgaagacgt
      301 gcgtgccatt gagcggaccg ccggcgccca gccaacaggc gagtgcgccg cgcgcgatca
      361 gcgcgtagag accggttgga ttgccctcgg cgataaaggc gctctcgaag ggggcggact
      421 ggccgcccgg gcgcggcaga tcggtgcgtt gcggcgccgc cgtttgtgcc gcggtcggcg
      481 gcgatgccac cggctgcggc gcggatgcct gtggcgcgcc gcccagcgta ggtagcgagc
      541 cgcagccccc gacggctgcc gccgcggcga tcgcaaaaaa caggaggcac aaacgctgca
      601 tgccccgtcg atccccgcta cgagcgcggc tcaccgcccg ccttgtttat cgccgcgctc
      661 ccccgagcgc gcgattcggc aaaagaacgg aatggacgct gccacaggag ggccgcgctg
      721 ggcaagaaac ggccacgaca ggcggcgctg gcccacacgc gagcgttgag acggcagggc
      781 cgggagccac cgtcagcagg tgcgggcgcg cgatggtgaa cccttggctc agttcagcat
      841 caggccgccg cgctcgccgc tgagatcgtg ccgatcgccc tcgcgcgtcg tcacgggctc
      901 gagcttgctg agttcggttg ccatttcctc gtgctcgcgg cgagcggcat cgagtttggc
      961 gcgcaagtcg gcgaccgagt tgagaaggtt catgcgccgc agcgaagcgg ccttggcaaa
     1021 cgtcgaatag gcaaagtgcg aggtgtcttt gatgccggtg cgctcctcct cggccgcaat
     1081 ctggcgcgcc agatcgtggg ccatgcgctc gaagtccccg atcatcgact ccatcgaaac
     1141 aaccgtgcgc gccttttcgc tgacttcaaa tcgtttcagc cgcaacgctg cgtcacgtgg
     1201 cttcatgact cactaccccc aaagtcccgc gttccatcct caccatcggc cggtcgatcg
     1261 cgcgctggcg cgcgccagga ccatacgcaa gccgtgtgcc acctgcacac ggatggctca
     1321 ccggccgaag cgttcgagcg gtgggcgacg gtgcatctcg cttcctttcg actaacttat
     1381 acatgagcca ttaagttgcg gtaaagcaac gcgtaattgc cggagcggag gacgtgtgcc
     1441 gcagccgcta cttttttttg ctgcgcctct agggcctgag tcgcaaatca gctagaagac
     1501 gaatgcggtg cgcagaaacc tgcctggctg gtgtcccaag ggtcaacgga cacagccaag
     1561 ctcaagcttc tgcgctgggg gtgaattcag ctgggtcatt tgtgtgccgt cttggcgcgc
     1621 ggcgggggct tcggcttctc gttgggccgc ggtatctgtt tgtgtacggc acaatgtccg
     1681 acgctgccgg ggtcgatgcc gcaagctttg ctgttctgaa atgagcgtac ttgtaaggca
     1741 aaattaggct ttgttaacct ctggcgacta gcctcgggaa aggaaggcta ggcgagttca
     1801 gccggcggcg gcctcttcaa acatcgggca gcggaccccg cacggggtct gggagagagg
     1861 attgacatgc gggtccttct gatcgaggac gacagcgcaa cggcgcagag catcgagctg
     1921 atgctgcagt ccgagggttt caacgtctac acgaccgacc ttggggaaga aggcgtcgac
     1981 ctcggcaaac tctacgatta cgacatcatt ctgctagacc tcaatctgcc ggacatgagc
     2041 ggctacgagg tcttgaaaac cctgcgcgtc agcaaggtgc agacgccgat cctgatcctg
     2101 tccggcctgg ccggcatcga ggacaaggtg cgcggcctgg gcttcggcgc cgacgactac
     2161 atgaccaagc ccttccacaa agacgaactc gtggcgcgca ttcacgccat cgtgcgccgc
     2221 tccaagggtc atgcccagtc ggtgatcgag accggcgagc tcaaggtcaa tctcgatacc
     2281 aagaccgtcg aagtccacaa tcagcgcgtg cacctgacca gcaaagaata ccaaatgctg
     2341 gagctgctct cgctgcgcaa gggcacgacg ctgacaaagg aaatgttttt gaaccacctc
     2401 tacggcggca tggacgagcc cgaactcaaa atcatcgacg tctttatctg caagttgcgc
     2461 aagaaactgc aggcggcgac cggtggtcag cactacatcg agacggtttg gggacgtggc
     2521 tacgtgttgc gcgatccagg tgacgaacac gtcgcggctt gagtgccttt ttctgcttct
     2581 tcgcttcacc tcacaagggg gccctcgggc tcccttttct ttgcctcgct cgctgcccca
     2641 aaggtttcgc ctttgccgcg ggctacgccg gtttcgcggc gggccaccgg cgcttccctg
     2701 cgtgacgctc cttgcaccga gacgttcggc gtgctcctgt ccccagcgca aaacaactca
     2761 ccgccgagcc atgccgcgtg tcacgccagg tcgctactcc cgttgccgga cacctcatca
     2821 cctgcctcgt caaggtcggt gatcgtgtcg aggtcgatac cgaggttggc atcgtcgaag
     2881 ccatgaagat gcatataccg gtcgtggccg aacagtctgg ccgcgttgcc aaatggctcg
     2941 tcagcgaaaa caccacggtg gccgaaggcc agcccctgat cgagctggtt tgaggcggcg
     3001 cccccggcgc cccgagttat gccgccgccg gcgacgggtc cttgcccctc tcggcggcat
     3061 gcgccagact gccgggcatt gtcgtggcat gcggacgtgg aaggagtgtc atggccaata
     3121 gcgcaacact gaaggttcgc gtcaatagag acaaatgcca ggggcacaat cgctgcaagg
     3181 cgatcgcgcc ggagctgttc gagctcgacg agttcggcaa cgcccgcgcc gccggcgacg
     3241 gccgcgtgcc gacgggcctg gaggagaagg cctacctcgc caagtcgaac tgtcc
//
LOCUS       DASTMT010000454         2663 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_3543096, whole genome shotgun sequence.
ACCESSION   DASTMT010000454 DASTMT010000000
VERSION     DASTMT010000454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2663
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3543096"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            271..510
                     /locus_tag="VFR00_10590"
     CDS             271..510
                     /locus_tag="VFR00_10590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0159754.1"
                     /translation="MQIVYDLAPVIAEIISGHCAGTRAREDFVHACLHGDWHEAKVMV
                     EGMLAEPWHLIGHQERRLREFLDLLELRDGTLVSQ"
     gene            950..2122
                     /locus_tag="VFR00_10595"
     CDS             950..2122
                     /locus_tag="VFR00_10595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015668339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0159755.1"
                     /translation="MTTTRWLAAPGLLATSLLLAATAQAQELKVGIMAVLSGPQAVLG
                     GQLRDGFNLGVKHAGGKLGGLATSVVVQDDELKPDVAVGKVKQLIERDKVDFVVGPVF
                     SNILLAIHKPVAEGKVFLISPNAGPSPLAGKGCSAFFFSTSYQNDQPPEVLGKYAQDK
                     GYKKAFLLAPNYQAGKDMISGFKKQFKGEIADELYTPLGQLDFSAELAKIASAKPDAF
                     FVFMPGGMGVNLVKQYRQAGLESIPFLSVFTVDESTLPAQQDAAVGFFSGMTWAPNTD
                     TPENKRFVADFEKEYGYVPGSYAMQAYDAAQLIGSAVKAVGGQLADKDALRAALQKAD
                     FKSVRGAFKFANNGFPVQDFYLVRVAKRADGKFQTEIVEKVFSNAVDSYAGDCPLK"
     gene            2244..>2663
                     /locus_tag="VFR00_10600"
     CDS             2244..>2663
                     /locus_tag="VFR00_10600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018699407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEU0159756.1"
                     /translation="MSASLFVVQLFNGLQLGILLFLVAAGLTLVFGVMDFINLAHGVQ
                     YMLGAYLAVTAYGFTGNFFLSVLLALLASLLFGLVLELLVFRHLYGRDHLEQVLATFG
                     IIMLLNQGVKLLWGAAPLNMPIPDLLSGSVPLMDGLRY"
BASE COUNT          464 a          859 c          835 g          505 t
ORIGIN      
        1 gaaggctttg tggggcgccg ctcaagccgg cgccccacgc tcgctgccca agctggcagc
       61 gcgattgggc cgcggcgcgg gcgttgcctg gcgccgttcc ccaaccagcg gaaaagttcc
      121 gcctgcaccc ggcggcacaa cgtctcgtta gatcccaagt gaagccgcgg ctgcaaaggc
      181 gtggccgcgt ggcactctcg aagatcggaa cgcaacgcga ctttcctggt tcttagcagc
      241 gggccggcgc tgccgttcaa cgaggttgcg atgcaaattg tctatgacct tgctcccgtg
      301 atcgcggaga tcatttccgg ccactgcgcc ggcacccggg cgcgcgagga tttcgtgcat
      361 gcctgcctgc acggtgattg gcatgaggcc aaggtcatgg tcgaaggcat gctggcggag
      421 ccgtggcatt tgatcggtca ccaggaacgg cggctcagag agtttctcga cctcctcgag
      481 ctccgcgatg gcaccctggt atcgcaatag accgcggcct gttcccttgt ttcgatgctg
      541 ccgatcagcg gttttgggcg cgggatgaga ggtgtgaact atcgtccaca tcaccgcgcc
      601 gcaagcccac cgacggctgc tcgcagcctt tgagcgggag cagggagtgc cttgcaccgt
      661 cggcaagtcg ggccaaggag caagagccaa caaaatgtcg gcggaccgtt ccgccccacg
      721 gggtgggcaa cggctacggc gcgcggctgg cccggccaag acatcgggct catacgacag
      781 gcctaaccgc gctctctctc tctctctctc agacccctcg gctgccgagc gggtgccgcc
      841 agcccggaca ttgccgccgc cacggcgggg ccctaaagtc ctcggcggga cggctgcgga
      901 gcggtctcgc cacggccagg cggctccgca aggtatcggg atgacacaaa tgacaacgac
      961 acgctggctc gcggcgccgg gcctgttggc gacctcactg ctcctcgccg caacggcgca
     1021 ggcccaggag ctcaaggtcg gcattatggc ggtgctgtcg gggccgcagg ccgtgttggg
     1081 tggacaattg cgcgacggct tcaatctcgg ggtcaagcat gccggcggca agctcggcgg
     1141 cttggcgacc agcgtcgtcg tgcaggatga cgaactgaag ccggacgtcg ccgtcggcaa
     1201 ggtcaagcaa ttgatcgagc gcgacaaggt cgacttcgtg gttggaccgg tgttctccaa
     1261 tatcctgctg gcgatccaca agccggtcgc ggaaggcaag gtattcctga tcagcccgaa
     1321 cgccgggcct tcgccgctcg ccggcaaggg ttgctccgcc tttttcttct ccacctccta
     1381 tcagaacgat cagccacccg aggtgcttgg caagtacgcg caggacaagg gctacaaaaa
     1441 agcctttctg ctggcaccca actaccaggc cggcaaagac atgatcagtg gcttcaagaa
     1501 gcaattcaaa ggcgagatcg ccgatgaact ctacacgccc ctgggccaac tcgatttctc
     1561 ggccgagctc gccaaaatcg ccagcgccaa gcctgacgcc ttcttcgtct tcatgcctgg
     1621 cggcatgggc gtgaacctgg tcaagcaata tcgccaggcc ggacttgaaa gcattccctt
     1681 cctttcggtc ttcaccgtcg atgaatcgac gctgcccgcc caacaggacg ccgcggtcgg
     1741 cttcttctcc ggcatgacct gggcgcccaa caccgatacg cccgagaaca agcggttcgt
     1801 cgccgacttt gagaaagagt atggctatgt ccccggcagc tacgccatgc aggcctatga
     1861 cgccgcgcag ttgatcggca gcgccgtcaa ggccgtgggg ggacagctcg ccgacaagga
     1921 cgcgctgcgc gcggcgctgc aaaaggccga cttcaagtcc gtgcgcgggg cattcaagtt
     1981 cgccaataac ggttttcccg tgcaggattt ttatctcgtg cgggtcgcca agcgcgccga
     2041 tggcaagttc cagaccgaga tcgtggagaa ggtgttcagc aatgccgtcg acagctatgc
     2101 cggcgattgt ccgctaaagt aggccagccc tgatctcgac ggagagccct ccgcgggacc
     2161 tgggccggtt atcgaacaac cggtgcgcgc ccgatctgca aaacgccgtc gccgcgcccg
     2221 ccgccgccgc ctagggtctc gcgatgtctg cttcgctgtt tgtcgtgcaa ttgttcaatg
     2281 gcttgcagct cggcatcctg ttgttcctgg tggcggccgg cctcacgctg gtgtttggcg
     2341 tgatggactt catcaacctc gcgcacggcg tccagtacat gctgggtgcc tacctggcgg
     2401 tgacggccta cgggttcacc gggaattttt tcctcagcgt gctgctggcg ctgctggcat
     2461 ccctgctgtt cggcctcgtg ctcgagctcc tggtctttcg ccacctttat gggcgcgacc
     2521 acctggagca ggtgcttgcc accttcggca tcatcatgct cctcaaccaa ggcgttaaac
     2581 tgctgtgggg tgcggccccg ctcaacatgc cgatcccgga tctgctgtcg ggatcggtgc
     2641 cactgatgga tgggctgcgc tat
//
LOCUS       DASTMT010000455         2136 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_5383666, whole genome shotgun sequence.
ACCESSION   DASTMT010000455 DASTMT010000000
VERSION     DASTMT010000455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2136
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5383666"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..603
                     /locus_tag="VFR00_10605"
     CDS             <1..603
                     /locus_tag="VFR00_10605"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEU0159757.1"
                     /translation="ADAAIMGARSAARAAQSLQELESTLAGFDGCGLKATAKNLCFYR
                     GAPRSTLMLIGEAPGRDEDLEGKPFVGRAGQLLDKMLAAIGLSEADVHITNIVYWRPP
                     GNRTPTPQEAQVCRPFLERQIELVAPKVIALLGGAAAKHLFDVTEGIMRIRGKERAIQ
                     IGAHSAKAMATLHPAYLLRTPAAKRLAWRDLMAVQALLRG"
     gene            757..1296
                     /gene="moaB"
                     /locus_tag="VFR00_10610"
     CDS             757..1296
                     /gene="moaB"
                     /locus_tag="VFR00_10610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HEU0159758.1"
                     /translation="MAKIDEKRPFLAVRIAVLTVSDTRSAAEDKSGDSLAEMIRAAGH
                     AVADRAIVRDEVASIEAKVKGWIDNPAIDVVITTGGTGFTGRDVTPEAIRPLFEKEID
                     GFSTVFHMISFQKVATSTIQSRACAGLAHGTYIFCLPGSPGACKDAWEGILRHQLDNR
                     HRPCHLVEIMPRLEEHRAR"
     gene            1286..1888
                     /locus_tag="VFR00_10615"
     CDS             1286..1888
                     /locus_tag="VFR00_10615"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU0159759.1"
                     /translation="MPGDRGAAPVGPPGPEIIAVAGPLPAARLAQVEAIFFEASGRRF
                     EPGPARDAFRQRWLGRYLDAPGDPVLLAVAGATVAGYLIGSLEDPAEQPRFADLEYYA
                     THFAAHCRRFPAHLHINLAPAYRGHGLGQALIAAFAAQAMAAGVAGVHVVTGKGMRNV
                     RFYERCGFEFVASAPWNGREVVLLGKELAAGRPTQAKDDS"
     gene            1888..>2136
                     /locus_tag="VFR00_10620"
     CDS             1888..>2136
                     /locus_tag="VFR00_10620"
                     /inference="COORDINATES: protein motif:HMM:NF016221.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HEU0159760.1"
                     /translation="MRALRAMVWLAPLTLAALTLGQDTAVAQVAEYHAGKIKIVAPWT
                     RATPESAKVGGGFMTLINTGSEPDRLIAGSTEVSEGLEI"
BASE COUNT          375 a          706 c          702 g          353 t
ORIGIN      
        1 gccgatgccg ccattatggg cgcgcgctcc gctgcccgtg ccgctcagtc gttgcaggaa
       61 ctggaaagca ccctggcagg cttcgacggc tgcggcctca aggccacggc caagaaccta
      121 tgcttttacc gcggagcgcc gcgctcgacg ctgatgttga tcggggaggc acccgggcgg
      181 gatgaggatc tcgaaggcaa accgttcgtg ggccgcgccg gtcagctgct ggacaagatg
      241 ttggccgcca tcggcctcag cgaagcggac gtgcacatca ccaatatcgt gtactggcga
      301 ccccccggca accgcacgcc gacgccgcag gaggcccagg tctgtcgccc atttcttgag
      361 cgccagatcg agctggtagc accgaaggtc attgccctgt tgggcggcgc tgccgccaag
      421 caccttttcg atgtgaccga gggcatcatg cgcattcgcg gcaaagaacg cgcgatccag
      481 attggcgcgc atagcgccaa ggcgatggcc actttgcacc cggcctatct gttgcgcaca
      541 cccgccgcca agcggctggc ctggcgagat ctgatggccg ttcaggcgtt attgcggggc
      601 tgaaccgtgc cgggcataca gatccgcaag ctttgcaggc gcaaagaggc aaccccccat
      661 gcttgcggcg cgcggacgcg cgccgcaagc acacgcacca gagcgacaac cgccgcgccc
      721 cagatctaga aagctgctcg agacggagac cgcgacatgg ccaaaatcga cgaaaaacgc
      781 ccgttccttg ccgtgcgcat tgcggtgctg acggtatcgg acacgcgcag cgcggccgag
      841 gacaaatccg gcgacagcct cgccgagatg atccgcgccg cagggcacgc ggtcgccgat
      901 cgcgccatag tgcgggacga ggtggcgagt atcgaggcca aggtgaaggg ttggattgat
      961 aaccccgcca tcgacgtcgt gatcacgacc ggcggcacgg gcttcacggg ccgcgatgtc
     1021 acgcccgaag ctatacgacc cctgttcgag aaagaaatcg acggcttttc gaccgtcttc
     1081 cacatgatct cgtttcagaa ggtggcaacc tcgaccatcc agtcccgtgc ctgtgccgga
     1141 ctagcccacg gcacctacat cttctgtctg ccgggctcgc ctggtgcctg caaggatgcg
     1201 tgggagggca tcctgcgcca ccagctcgat aaccgtcacc ggccctgcca tttggtcgaa
     1261 atcatgccgc gcctggagga gcaccgtgcc cggtgatcgc ggcgccgcgc cggtggggcc
     1321 ccccggcccc gaaatcatcg ccgttgcagg tcccctgccg gcggctcggc tggcccaggt
     1381 ggaggccata ttcttcgagg caagcggtcg ccgcttcgaa cccggaccgg cgcgggatgc
     1441 ctttcgccag cgttggctcg ggcgctatct cgacgcgccg ggggatccgg tcttgctggc
     1501 ggtcgccggc gccacggtcg ccggttacct gatcgggagc ctggaggatc ccgccgagca
     1561 gccgaggttc gccgatctgg agtactacgc tacgcatttc gcggcccatt gccgtcgctt
     1621 tccggcacat ctgcacatca atttggcgcc cgcctaccgc ggccacggcc tcggccaggc
     1681 gctgattgcg gcgtttgcgg cacaggccat ggcggccggt gtcgccggcg ttcacgttgt
     1741 caccggcaag gggatgcgca acgtgcgctt ctatgaaaga tgcggtttcg agtttgtcgc
     1801 ttcggcgccg tggaacggac gcgaggtggt tttgctcggc aaggagctgg ccgcgggccg
     1861 accaacgcaa gcaaaggacg acagctgatg cgtgctctca gggctatggt gtggctcgca
     1921 cccctcacgt tggccgcgct gacgctgggg caggacaccg ccgtcgcgca agtggccgaa
     1981 taccacgcgg gcaaaatcaa gatcgtcgcg ccgtggacgc gcgccacgcc cgaaagcgcc
     2041 aaggtcggtg gcggctttat gaccctgatc aacacgggca gcgagcccga ccgcttgatt
     2101 gcgggctcca cggaagtgtc cgaaggcctg gaaatt
//
LOCUS       DASTMT010000456         3211 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_4728288, whole genome shotgun sequence.
ACCESSION   DASTMT010000456 DASTMT010000000
VERSION     DASTMT010000456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3211
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4728288"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..823)
                     /locus_tag="VFR00_10625"
     CDS             complement(<1..823)
                     /locus_tag="VFR00_10625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007592767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoate--protein ligase family protein"
                     /protein_id="HEU0159761.1"
                     /translation="MVKASPRYTRYWRTYTPRRANALIEPPARSQDMPSPSRTAAAHR
                     VDLPPPFQLVRLREVGDAFRHASEIAADAGAGTFVYVGRFDLAEFAIVLEPEEPLLQA
                     RRAFYAGMVALREALLAQAPPERPITFAWPDAIHIDGGLIGGGRLGWPAGALEESAPA
                     WLVFGASIRTFGLGEREAGLHPLATALDQEGFEEAGSDRLIESFARHLMVAIDAWRAN
                     EFGSVMRSYLSHLSVEKGASPSLAGNGDLLIKWRGQREPDRHDLAAALAVPSWLDP"
     gene            992..2959
                     /locus_tag="VFR00_10630"
     CDS             992..2959
                     /locus_tag="VFR00_10630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002718839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEU0159762.1"
                     /translation="MPLDTAAVARGCRGAELTTARQLCRAELERFRTLAAKPEALTIA
                     CTQEAPLFEEVAAETGRAAPVTYVNVRETAGWSREASRAGAKMAALLAAAAEEMPPVP
                     LVEVESAGVVLIYGGDERAIEAGTFLQDHLDVTVLIKPPTDVAAGRNTEFPVVRGLVR
                     NARGHFGAFELTVDQFAEPAPSSRGKLAFGPGRDGARSSCDLILDLSGGVPLFAGKDL
                     IDGYLRADANDPAAVLKAVLKLRDLSGTFEKPRYVDFNADLCAHARSRIVGCNRCLNL
                     CPTEAIAPAGNHVAVDAVRCAGCGQCAAVCPTGAAAYALPPADALLRKLRALLTTYAQ
                     AGGEGAVLLIHDKAGADLLSALSHGGDGLAAHVLPLAVNEVTQVGLEAIAAAFAYGAS
                     AVRFLLRGRPIHDVAGLRQTIELSETILVGLGFAAAGVASIETDDPEALQAALASIVT
                     GRGARAAASFLPVGGKRSVLRQALRELHAVAPSPVPVLALPARAPFGAVELKVEGCTL
                     CLACVSACPTGALGDDPERPLLRFAEDACVQCGLCQATCPEKVITLKPQIDFRAATAA
                     ARVLKEEPPFACIRCGKPFGVKSTIERVAAKLEGKHWMYTGAPQRLEVVKMCDDCRVA
                     VVSEQDFDPYGSPPRQPVRTTEDYLKEREKG"
     gene            complement(2986..>3211)
                     /locus_tag="VFR00_10635"
     CDS             complement(2986..>3211)
                     /locus_tag="VFR00_10635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018270968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0159763.1"
                     /translation="TLAGLFGRRIATLPGYDFSPALKKLNIVWTPDTLSKLFELGPAA
                     FTPGSKMPEQKVGTEERTELIRFLEKATRE"
BASE COUNT          499 a         1082 c         1091 g          539 t
ORIGIN      
        1 cagggtcgag ccaggaggga acggcaaggg ccgcggccag atcgtggcgg tcgggctccc
       61 gttgtccgcg ccatttgatc agcaaatcgc cgttgccggc caggcttggc gaggcgcctt
      121 tttccacgct gagatgcgaa agatagctgc gcatcacgct gccgaattca ttggcacgcc
      181 aggcgtcgat ggccaccatc aggtggcggg caaagctctc gatcagtcga tccgagcccg
      241 cctcttcgaa gccctcttgg tcgagcgccg tggccagcgg gtgcaagccc gcctcgcgct
      301 cgcccaggcc gaacgttcgg atcgaggcgc cgaacaccaa ccacgccggt gcgctctcct
      361 cgagcgcgcc cgccggccaa ccgaggcgcc ccccgccgat caacccaccg tcgatgtgga
      421 tcgcatcggg ccaggcaaag gtgatcggcc gctccggcgg cgcctgcgcc agcaaggcct
      481 cgcgcagggc taccatgccg gcatagaagg cgcggcgcgc ctgcagcagc ggctcctccg
      541 gctcgagcac gatggcgaat tcggcgagat cgaagcgccc gacatagaca aaggtgccgg
      601 cgcccgcgtc ggcggcaatt tcgctggcat gccggaaagc gtcacccacc tcccggagcc
      661 ggaccagctg gaacggcggt ggcaggtcga cccggtgggc ggcagctgtg cggcttgggc
      721 tcggcatgtc ctgggagcgg gcgggcggct cgattagcgc attggctcgg cgcggcgtat
      781 aagtgcgcca gtaacgagta taacgggggc tggccttgac caacagcctt cccctctaag
      841 gcttcaggac cgcatggcaa agtcttcccg ctcgagcgcc gagagcggct ccacatctcg
      901 gccgcagccc ggcgcagagc caacccaagg cttggctccg gccgccggcc gcgaggctcg
      961 acgtatcgta ttgtgctcgt gtgaagacac catgccgctc gatacggccg ccgttgcccg
     1021 cggctgtcgt ggcgccgagc tgacgaccgc gcgccagctc tgccgcgcag agctcgagcg
     1081 cttccgaacc ctcgccgcca agccggaggc gctgacgatt gcctgtacgc aagaagcgcc
     1141 gctattcgag gaggtcgccg ccgagacggg tcgcgcagcc cccgtgactt acgtgaacgt
     1201 acgcgagacc gcgggctggt cacgcgaggc gtcccgcgcc ggtgcgaaaa tggcagcact
     1261 gctggcggca gcggccgaag agatgccgcc cgtgccgctg gtcgaggtgg aaagcgcggg
     1321 tgtcgtcttg atttacggcg gcgacgagcg cgccatcgaa gccggcactt ttctgcagga
     1381 ccacctggat gtgactgtcc tcatcaagcc gccgacggac gtcgccgcgg gccgcaatac
     1441 tgagtttcca gtggtgcggg gcttggttcg caacgcgcgc ggccatttcg gcgccttcga
     1501 actcaccgtc gatcagttcg ccgaaccggc tccgtcttcg cgcggcaagc tcgccttcgg
     1561 gcccggccgc gatggcgcgc gctcgagctg tgatctcatc ctggatctga gcggcggggt
     1621 gccgctgttt gcgggaaaag acctcattga cggctacttg cgcgccgacg ccaacgatcc
     1681 tgccgccgtc ttgaaggcgg tcctcaagct gcgcgacctg tctggcacgt tcgagaagcc
     1741 gcgttacgtg gacttcaatg cggacctgtg cgcccacgcc cgctcgcgca tcgtgggctg
     1801 caaccgttgc ctcaatcttt gcccgaccga agccattgca cccgccggca atcatgtcgc
     1861 cgtggatgcc gtgcgctgcg cggggtgcgg gcaatgcgcg gccgtctgtc cgactggcgc
     1921 ggcagcctac gccctgccac cggccgatgc tttgctgcgc aagctgcgcg cgcttttgac
     1981 cacctacgcc caagcggggg gcgagggcgc agttttgctc atccacgaca aggccggggc
     2041 ggacctgttg agcgcgctct cccatggcgg cgacggactt gccgcccacg tgctgccgct
     2101 cgccgtcaac gaggtgaccc aagtcggctt ggaagcgatc gcggcggctt ttgcctatgg
     2161 ggcctcggcc gtgcgtttcc tgctgcgggg acggccgatc catgacgtgg cgggtctgag
     2221 gcaaacgatc gagctctcgg aaaccattct ggttggcctc ggctttgccg ccgccggcgt
     2281 ggcgtcgatc gagacggacg atccggaggc cctgcaagcg gcgcttgcca gcattgtcac
     2341 ggggcgcggg gcgcgggcgg cggccagctt tctgcccgtg ggaggaaaac gcagcgtgct
     2401 acgccaggcg ttgcgcgaac tgcatgccgt ggcgccctcc cctgtcccgg tgttggcctt
     2461 gcccgcgcgg gcaccgtttg gtgccgtcga actcaaagtc gagggctgca cgctatgcct
     2521 tgcctgcgtt tccgcttgcc ccacgggagc gctcggcgat gatccggagc ggccgctgtt
     2581 gcgctttgcc gaggacgcct gcgtgcagtg cgggctgtgc caggccacct gtcccgaaaa
     2641 ggtgatcacg ctcaagccgc agatcgattt tcgcgcggct actgccgccg cccgcgtcct
     2701 caaggaggag cccccctttg cctgcatccg ctgcggcaag ccgttcggcg tcaagagcac
     2761 gatcgagcgc gtggcggcca agctcgaagg caagcattgg atgtataccg gcgcgccgca
     2821 gcgccttgag gtggtcaaaa tgtgcgacga ctgccgcgtc gccgtcgtct ccgaacagga
     2881 ttttgatccc tatggctcgc cgccgcgcca gcccgtgcgc accaccgagg attatttaaa
     2941 ggagcgcgaa aagggctgag cccggcaagg ctgctggccc ggcgtctatt ccctggtggc
     3001 tttctcgaga aagcggatga gctcggtgcg ctcctccgtg ccgaccttct gttcgggcat
     3061 cttgctgccc ggcgtaaagg ccgccgggcc cagctcgaac agcttggata gggtgtcggg
     3121 cgtccagacg atgttgagtt ttttcagcgc cggggaaaaa tcgtagcccg gcagcgtcgc
     3181 aatgcggcgg ccaaacaggc cagcgagcgt c
//
LOCUS       DASTMT010000457         1505 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_2234638, whole genome shotgun sequence.
ACCESSION   DASTMT010000457 DASTMT010000000
VERSION     DASTMT010000457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 1505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1505
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2234638"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..471)
                     /locus_tag="VFR00_10640"
     CDS             complement(<1..471)
                     /locus_tag="VFR00_10640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HEU0159764.1"
                     /translation="MNAKDMASAKAEAKTATLAIGSNQRAEMPIRSGTIGPDVIDVGR
                     LYRDTGCFTYDPGFTSTANCSSRITYIDGDQGVLLYRGYPIEQLAQQSSFIEVCYLLL
                     NGELPTKRGLETFSNTITRHTMVHEQMTRFFTGFRRDAHPMAVMCGSVGALSAFY"
     gene            complement(636..>1505)
                     /locus_tag="VFR00_10645"
     CDS             complement(636..>1505)
                     /locus_tag="VFR00_10645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008595581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HEU0159765.1"
                     /translation="NHAVVVDDHDMGITHVIRGSDHLTNAARQVQLYRAMAWEVPVMA
                     HIPLIHGADGAKLSKRHGALGVEAYRELGYLPEGLRNYLCRLGWSHGDDEIFSTEQAI
                     AWFDIADVNKAPARLDMAKLADVNSHYIRQASDEALIGRIKDLLAVTSGGEKSRARFE
                     AVGWDRLAAALPGLKPRAKTLLDLIDGAVWLISQRPLSLDDKAKKLLEGDARGLLGRL
                     KVELSQVADWQPAALEAAVKGFAAASGHKLGQVAQPLRAALTGRAVSPPVFDVMAVLG
                     RAETLARIQDQAA"
BASE COUNT          257 a          481 c          489 g          278 t
ORIGIN      
        1 atagaaggca ctgagcgcgc cgaccgagcc gcacatcacc gccatcggat gcgcatcgcg
       61 ccgaaagccc gtgaaaaagc gcgtcatctg ctcgtgcacc atggtgtggc gcgtgatggt
      121 attgctgaac gtctcgagcc cgcgcttggt cggcagctcg ccgttgagca gcaaatagca
      181 gacctcgata aaactcgatt gctgcgccag ctgctcgatc gggtagccgc gataaaggag
      241 cacgccctga tcgccatcga tgtaggtgat gcgcgaggag caattggccg tggaggtgaa
      301 gcccggatcg taggtgaagc agccggtgtc gcgatatagt cggccgacgt cgatcacgtc
      361 ggggccaatg gtacccgatc ggatcggcat ttccgcccgc tgattgctgc cgatcgcgag
      421 cgttgcggtt ttcgcttcgg ccttcgcgct tgccatgtct tttgcattca tgggcgtcat
      481 cctttggatc cagcgacgct gacgccaccc tgaggggccc cgcctccgat tctgggggca
      541 cagcaaactc accctctagt atatatattg cactgcggca agctatgcga agggtggagc
      601 aaatctgctt gcacggcccg cgttgcgccg catcgtcagg ccgcctgatc ctggatgcgc
      661 gccagggtct cggcgcgccc gagcacggcc atgacgtcga acacgggcgg tgataccgcc
      721 cgccccgtta gggcggcccg caggggttgg gccacctggc caagcttgtg gccgctggcc
      781 gcggcaaagc ccttgaccgc cgcttccagg gcggccggct gccagtcggc cacctggctc
      841 agttcgacct tgaggcgacc gagcaggccg cgggcgtcgc cctcgagcag cttctttgcc
      901 ttgtcatcaa ggctgagcgg ccgctggctg atcagccaga cggcgccgtc gatgaggtcg
      961 agcagggtct tggcgcgggg ttttagaccg ggcagggcgg cggccagccg gtcccagccc
     1021 acggcctcga accgggctct cgacttttcg cccccgctcg tcaccgccag caggtctttg
     1081 atgcgcccga tcagcgcctc gtcgctggct tggcgaatat aatggctgtt gacgtcggcg
     1141 agcttggcca tgtcgagccg cgccggcgcc ttgttgacat cggcaatgtc gaaccaggca
     1201 atggcctgtt cggtcgagaa aatctcatca tcgccatggc tccagccgag gcggcaaaga
     1261 tagttgcgca aaccctcggg caaatagccg agctcccgat aggcctccac gcccagcgcc
     1321 ccgtgccgct tggagagctt ggccccgtcc gcaccatgga tcagcgggat gtgcgccatg
     1381 accggaacct cccaagccat ggcgcggtaa agttgcactt gccgggcggc gttggtgaga
     1441 tgatcggagc cgcggatgac atgggtgatg cccatatcat ggtcgtcgac caccacggcg
     1501 tggtt
//
LOCUS       DASTMT010000458         3792 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_3217502, whole genome shotgun sequence.
ACCESSION   DASTMT010000458 DASTMT010000000
VERSION     DASTMT010000458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3792)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3792)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3792
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3217502"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            667..1653
                     /locus_tag="VFR00_10650"
     CDS             667..1653
                     /locus_tag="VFR00_10650"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009764780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family oxidoreductase"
                     /protein_id="HEU0159766.1"
                     /translation="MQTFKALLASKTEAGPSLAWQELTEADLMEGDVTVAVSHTTINY
                     KDGLALTGKAPVIRRWPMIPGIDLAGSVVASSHADFKAGDPVVLNGWGAGETHYGGYA
                     ARARLKGDWLIKRPPQLTAAEAMAIGTAGYTAMLALLALERNGLRPEQGPVVVTGAAG
                     GVGSIAIILLARLGYRVIASTGRTSEADYLTELGAAEVIDRAQLAGPARPLGKERWAG
                     GIDAVGSHTLANLLSMTQYGGTIAACGLAQGMDLASSVAPFILRGVTLAGIDSVMAPK
                     ALRQEAWGRLARDLDKAKLAAVTTSRPLAEVSTLAPDILAGKIRGRVVLAVG"
     gene            1764..2720
                     /gene="lipA"
                     /locus_tag="VFR00_10655"
     CDS             1764..2720
                     /gene="lipA"
                     /locus_tag="VFR00_10655"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019173454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HEU0159767.1"
                     /translation="MVTVLDTRQPRHPEKAHRPDTQVLRKPSWIRVKVPAASPQIAET
                     RRILRQHALTTVCEEAACPNLAECWAGRHATMMIMGDTCTRACAFCNVRTGRPLALDA
                     GEPERVALAVAELGLQHIVITSVDRDDLADGGASHFARTISAIRAKTPHTTIEVLTPD
                     FLRKEGALETVLAAAPDIYNHNLETVPSLYLRIRPGARYFHSVRLLQRVKELAPGMFT
                     KSGIMVGLGEEREEVLQLMDDLRSAEVDFLTIGQYLQPTRKHAEVARFVPPEEFEAYR
                     RVALSKGFLLVAASPLTRSSYHADRDFARLRAARAAEAGQAS"
     gene            2779..3249
                     /locus_tag="VFR00_10660"
     CDS             2779..3249
                     /locus_tag="VFR00_10660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020095415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RatA family
                     toxin"
                     /protein_id="HEU0159768.1"
                     /translation="MPSFRTTHRVRFTARQMFDLVADLESYPAFLPLCEALVVRRREQ
                     RAGTSVLWADMTVGYLAIRETFTSIVELDPAGLTVLARGAPEGRGPFQRLENRWSFRP
                     ADGGSDVDFFISYTFKSLMLQALVGSLFDRVFRRYTRAFEERAQRVYGSGAPAA"
     gene            complement(3272..3766)
                     /locus_tag="VFR00_10665"
     CDS             complement(3272..3766)
                     /locus_tag="VFR00_10665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HEU0159769.1"
                     /translation="MHLAPLIDEANRLIAACRAHGTRIVTAESCTGGLISALLTEGAG
                     SSDVLERGFVTYSNAAKEQVLGVPGALLAQHGAVSAEVARAMAAGALARSAATLAVSV
                     TGIAGPGGGSALKPVGLVYLGMASLGQPTTHLECRFGDIGRSAVRLNCVKAALGLLWQ
                     ALPA"
BASE COUNT          628 a         1279 c         1271 g          614 t
ORIGIN      
        1 gatgcacgcg ccaaaaatgc gggagggcgc gcggattgct ctcgcgcgcc ctcgaatggc
       61 tcactgggat ttagcgcgat cccctctccc gcggatcgcc gctccccctc aaaacatgcc
      121 aaacacgact gcagtcagaa aggcgaagga tatcagggcg gcaagcacat tgagcctggt
      181 ccgtagttcc atcgctctgc ctccggcgag tgttgacgca agttgccccg cgccaacgtc
      241 ggaatctagg gatcggttcg cgagctgcca agcgaaatcg ctgctgcagc gcaataattc
      301 gatgttatcg aatgaagttt ccggtttgcc tcaccgattg atgcacaaca agcacgtcaa
      361 cactagtctt atacaagagg ttaatctaag taaacgcgct gtattaacct tatattcaga
      421 ttgtagagct tgcgagcaac cgaatgctgc cccaatagcg tcattcaagt tcttgtacag
      481 cccgaaagtc gctggggcgg cgtgcgcggt cgcatccgcg tgccctcagt tgcacagttg
      541 ctacagaatg acggtggggg cgggcgggcg cggcccggcc cgggcctcga cacccgcacg
      601 ccgatggcct accgagggcc gcagcaagcc ggcgtgggcg cgaatcagcg tggttggaga
      661 ggtgggatgc agaccttcaa ggcgttgctc gccagcaaaa ccgaggccgg accgagcctc
      721 gcctggcagg agctgaccga ggccgacctg atggagggcg acgtcaccgt cgcggtcagc
      781 catacaacta ttaactacaa ggatggtctt gccctgaccg gcaaagcgcc ggtgatccgg
      841 cgctggccga tgatccccgg catcgacctg gccgggagcg tcgtcgcctc gagccatgcc
      901 gatttcaagg ctggtgatcc ggtcgtgctc aacggctggg gtgcggggga gacccactac
      961 ggcggctatg ccgcgcgcgc ccgcctcaag ggcgattggc tgatcaaacg gccaccgcag
     1021 ttgaccgccg cggaggcgat ggcgatcggc acggcgggct acacggccat gctcgccctg
     1081 ctggcgctcg aacgcaacgg cctgcgcccc gaacaggggc ccgttgtcgt caccggcgcg
     1141 gccggcggcg tcggcagcat tgccattatc ctcctcgccc gcctgggcta ccgtgtgatc
     1201 gcctcgaccg ggcgcacgag cgaagccgac tatttgaccg agctcggtgc ggccgaggtg
     1261 atcgaccggg cgcaactcgc cgggccggcc cgcccgctcg gcaaggagcg ctgggccggc
     1321 ggcatcgacg ccgtcggcag ccacacgctg gccaacctcc tctccatgac ccagtacggg
     1381 gggacgatcg ctgcctgcgg cctggcacag ggcatggatc ttgcctcctc cgtcgccccc
     1441 ttcatcctgc gcggcgtgac gctcgccggg atcgattcgg tgatggcgcc gaaggcgctg
     1501 cggcaggagg cctggggccg gctcgcgcgc gacctcgaca aagccaagct cgccgccgtc
     1561 accaccagcc ggccgctggc ggaggtcagc acccttgccc cggacatcct ggcgggcaaa
     1621 attcgcggcc gggtggtgct cgccgtcggc tagcccgcgc tgctgcaccc cgctcggctt
     1681 gatttcaggg ggcggggggc gcacatcttg gccggcggcg tccgcgcctt cgcgacgccc
     1741 gccaccgtca gctaggatct tttatggtca ccgtcctcga cacccgccag ccaaggcatc
     1801 ccgagaaggc acaccggccc gatacgcagg tgctgcgcaa gcccagctgg atccgggtca
     1861 aggtgccggc ggcctcgccg cagatcgctg agacccggcg gatcctgcgc cagcacgcgc
     1921 tcaccaccgt gtgtgaggag gccgcttgcc cgaacctggc ggaatgctgg gcgggacggc
     1981 atgccacgat gatgatcatg ggcgatacct gtacgcgcgc ctgtgccttt tgcaacgtgc
     2041 gcacgggacg gccgctcgcg ctcgatgccg gcgaacccga aagagtggcc ctggcggtcg
     2101 ccgagctcgg cctccaacac atcgtcatca cctcggtcga ccgtgacgat ctggccgacg
     2161 gtggcgcttc ccactttgcc cgcaccatca gcgccattag ggcgaagacg ccgcacacca
     2221 cgatcgaggt gctgaccccg gatttcctgc gcaaggaggg ggcgctcgag acggtgcttg
     2281 ccgccgcgcc cgacatctac aaccacaacc tcgagacggt gccctcgctg tatctgagga
     2341 tccgaccggg ggcgcgctat ttccattcgg tgcggctgct gcagcgggtc aaggaactgg
     2401 cccccggcat gttcaccaaa tccgggatca tggtgggcct cggcgaggag cgcgaggagg
     2461 tgctgcaact catggatgat ctgcgcagcg ccgaagtgga ctttctcacc atcggccaat
     2521 acctgcagcc cacgcgcaag cacgccgagg tcgcccgctt cgtgccgccg gaggagttcg
     2581 aagcctatcg ccgggtggcg ctgtccaaag gctttctgtt ggttgccgcg agcccgctga
     2641 cgcgctcgtc ctatcatgcg gatcgggatt ttgcccgctt gcgcgcggcg cgcgcggccg
     2701 aggcgggaca ggcgagctga cggtcgagga gcgggcctcc ttgtcccgcc gcgagaaggg
     2761 gcaaggagcg ggggcggcat gccatccttt cgcacaaccc accgagtgcg tttcaccgcg
     2821 cgccagatgt tcgacctcgt ggccgatctg gagagctacc cggccttcct gcccttgtgc
     2881 gaggcattgg tggtcaggcg gcgcgagcag cgggcaggca ccagcgtgct gtgggccgac
     2941 atgacggtcg gctatctcgc catccgggag accttcacca gcatcgtcga gctcgacccg
     3001 gctggcttga cggtattggc gcggggcgcg cccgaggggc gcggcccctt tcagcgcctg
     3061 gaaaaccgct ggagcttccg tcccgccgac ggcggctcgg acgtcgactt cttcatcagc
     3121 tataccttca aatcgctgat gctgcaggcg ctggtcggca gcctgttcga tcgcgtcttt
     3181 cgccgctaca cgcgcgcctt cgaggaacgc gcccagcgcg tctacggcag cggcgcgcct
     3241 gccgcttgag gcggatacac gtgctgcgcg ctcaggctgg cagggcttgc cagaggaggc
     3301 cgagcgcggc cttgacgcaa ttgaggcgga cggcgctgcg cccgatgtcg ccaaagcggc
     3361 attccaggtg tgtcgtgggc tggccgaggc tcgccatccc caagtacacg aggccgacgg
     3421 gcttgagcgc gctaccgccg ccggggccgg caatgcctgt cacggaaacg gcgagcgtgg
     3481 cggccgaccg cgccagcgca cccgctgcca tggcgcgcgc gacctccgcg ctcaccgccc
     3541 cgtgctgggc cagcagcgcg ccggggacgc ccagcacctg ttctttggcg gcattggaat
     3601 aggtgacaaa gccgcgttcg aggacgtcgg aggagccggc gccttcggtc agcagcgctg
     3661 agatgagccc gccggtacaa ctctccgcgg tgacgatgcg tgtcccgtgc gcccggcagg
     3721 cggcaatgag gcggtttgct tcgtcgatca ggggcgccag atgcatcagg cctcgcaaac
     3781 aagaacggta tc
//
LOCUS       DASTMT010000459         5758 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_3217708, whole genome shotgun sequence.
ACCESSION   DASTMT010000459 DASTMT010000000
VERSION     DASTMT010000459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 5758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5758
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3217708"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            110..1720
                     /locus_tag="VFR00_10670"
     CDS             110..1720
                     /locus_tag="VFR00_10670"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEU0159770.1"
                     /translation="MQTTTFAELGLSAKVQAAIEAAGYTNPTPIQQKAIPVAVSGRDV
                     LGIAQTGTGKTAGFVLPMLARLETGRARARMPRSLILAPTRELAAQVAQSFEKYGVNH
                     KLSVALLIGGVSFDDQLRKLDRGVDVLIATPGRLLDHFGRGKILLTGVEILVIDEADR
                     MLDMGFIPDIERICKLLPPARQTLFFSATMPPEIQRLVDTFLRDPARIEVARPATTAN
                     TITQLFRYCPYSEDWQKREVLRELIRAENVKNAIIFCNRKRDVAILLKSLQKHGFNAG
                     ALHGDMDQMSRMATLDAFREGRIQFLAASDVAARGLDIPDVSHIFNFDVPWQSDDYVH
                     RIGRTGRAGKEGRSLTIVTPDDIRQLKDIEKMLGATVTWIGAGPSAEDLASGAKRRRG
                     RRPGRRQAPGAEARGNGHARGARNGAPSPQHARNGSGHEPEHAAQASAKPRERAAPAR
                     AQTRHGEPRPPAAGKPQRGDRPQHGRGERRGNGEAQPSRQESKARQPWEPRPPRPQRY
                     REDRTPSPVGLGDHVPAFLKKAPRPATR"
     gene            complement(1820..2014)
                     /locus_tag="VFR00_10675"
     CDS             complement(1820..2014)
                     /locus_tag="VFR00_10675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018431063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase family protein"
                     /protein_id="HEU0159771.1"
                     /translation="MPEVYVHAIEGRSIEQKRALVKDITDAVVRHFNVKPDAVMVQIM
                     ESPKDSKAKGGFLFSDRPPG"
     gene            2227..3396
                     /locus_tag="VFR00_10680"
     CDS             2227..3396
                     /locus_tag="VFR00_10680"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020181372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEU0159772.1"
                     /translation="MLQLVPNLQRLGTETAFEVLARAAELQRQGKDVINLGIGQPDFQ
                     TPPHIVEAAVKALRDGHHGYTPAQGILPLREAVAADLKRRHGVDVSPDTIVIMPGGKP
                     TMFFAILMFGQPGADIMYPDPGFPIYRSMIQYTGARPVPIALLEKNEFAFTADQVLAQ
                     ITPQTSLIILNSPNNPCGGAVPAEEVKRLVIGLEKHPHVAVLSDEIYSQMLYGGRKHE
                     SFLRYPSLRDRLIVLDGWSKTYAMTGWRLGFSVWPQGLVEHVVRLCVNNHSCVNAPTQ
                     YAGIAALEGPQDAVGTMVAAFDERRRTIVPLLNQLPGIRCLDPGGAFYVFPNVTGTGM
                     TAKDLQNLMLEKAGVATVAGTSFGIHGEGYIRFSYANSVENIREAVRRLAALLRD"
     gene            3402..4154
                     /locus_tag="VFR00_10685"
     CDS             3402..4154
                     /locus_tag="VFR00_10685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018646848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU0159773.1"
                     /translation="MDLQIRDRTALVTGASIGIGRGVALGLAREGARLALVARRRELL
                     EEVAAEVMAAGGSRPVLISEDLLQEGAPARIARAALAGLGSVSILVNNAGGSRPFKLD
                     ASEEAWHEAMTLNFTRQRQLTHSLLDTMISAKWGRIITMTGKSEPEGINGAFSAKAAV
                     HAWAKGLSREVGRYGITVNCIPPGRIMSEQIRRNYPPEYRQWQAEHEIPVGRYGEPED
                     VANLVCFLASPLAAYITGTVIPVDGGLRRYQF"
     gene            4310..4828
                     /locus_tag="VFR00_10690"
     CDS             4310..4828
                     /locus_tag="VFR00_10690"
                     /inference="COORDINATES: protein motif:HMM:NF019430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HEU0159774.1"
                     /translation="MKMRARFVAPALLAAAAIPTIVLAQSQQPQDAQSRPERQAQRPQ
                     LSPEARARLQEGRFAMIKETLKLNDAQRKLWEPVEAQLRASAAERQKLREERQAARQQ
                     NRAQPALPERLDRASERMAKRAARMKAFAEVFKPFYESLDEEQKAVAGIVLRQARGGG
                     HRGHGHRWAMQR"
     gene            complement(4993..>5758)
                     /locus_tag="VFR00_10695"
     CDS             complement(4993..>5758)
                     /locus_tag="VFR00_10695"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HEU0159775.1"
                     /translation="TRASTPRPRQPILAPAAGRDQRARAPRAAGSTNENGGAVADTKG
                     LLVVQEDTIIKGEVRNCRQIEVFGYVEGKVAAESAVIHAGGRCFGTLRSQTAEVSGTL
                     QGNVFVKNLINIRSSAEVSGNVHYGALAMEVGGNLSAEVRNVPPALAGDFTVTVERGR
                     SVKIELEDLSAVDPDDAPTDLIFRVGNARNGFVALASAARQPVTQFSQADLAAGNIVF
                     THSGAPGKTAGFSVVVTDHAGASSGAPQQVVVIVRG"
BASE COUNT          960 a         1987 c         1884 g          927 t
ORIGIN      
        1 ttcccgttag gccctttgcg gctagtcccc ctgcgctcag gttgctgatg tttgcgccca
       61 cacgggttgc gcgagcgttg gcttatgagg cagacggaga accgttcttt tgcagacaac
      121 gacgttcgct gagctcggtc tcagcgcgaa ggtacaggcg gccatcgagg ccgccggcta
      181 caccaatccc acgccgatcc aacaaaaggc cattccggtc gcagtttccg ggcgcgatgt
      241 gctcggcatt gcccagacgg gcacaggcaa gacagccggg ttcgtgctgc cgatgctggc
      301 ccgcctcgaa accggccggg cccgggcgcg catgccgcgc tcgttgatcc tggcgcccac
      361 ccgcgagctt gccgcccagg tggcgcagag cttcgagaag tacggcgtca atcacaaact
      421 gagcgtcgct ctcttgatcg gcggcgtttc cttcgatgat cagctgcgca agctcgaccg
      481 aggcgtcgat gtcttgatcg ccacgcccgg gcgcctgctc gatcacttcg gccgcggcaa
      541 gattctcctg accggcgtcg agatcctggt gatcgacgag gccgatcgca tgctcgacat
      601 gggctttatc ccggatatcg agcggatctg taagctgttg ccgccggcgc gacagaccct
      661 gtttttctcg gccaccatgc cgcccgagat tcagcgcctc gtcgacacgt ttttgcgcga
      721 tcccgcgcgc atcgaggtgg ctcgaccggc caccaccgcc aacaccatca cccagctctt
      781 tcgctattgt ccctactcgg aggattggca aaagcgtgag gtgctgcgcg agctgatacg
      841 ggcggagaac gtcaagaacg ccatcatctt ctgcaatcgc aagcgcgacg tcgcgatcct
      901 gctcaaatcg ctgcagaagc acggcttcaa tgcgggcgcg ttgcatggcg acatggatca
      961 gatgagccgt atggcaacgc tcgatgcttt ccgcgagggg cgcattcagt tcctggcggc
     1021 cagcgatgtc gccgcccgtg gtctcgacat tcccgacgtc agccacatct tcaatttcga
     1081 cgtgccttgg cagtccgacg attacgtgca tcgcatcggc cgcaccggcc gggccggcaa
     1141 ggaggggcgc tcgctcacca tcgtcacgcc cgacgatatt cgtcagctca aggacatcga
     1201 gaaaatgctg ggtgccacgg tcacctggat cggcgcgggc cccagcgcgg aggacttggc
     1261 gtccggcgcc aagcgccgcc gtggccgccg cccaggcagg cggcaagccc ccggcgccga
     1321 agcgcgcggc aacggccatg cccggggcgc ccgcaacggc gcgccctctc cgcagcacgc
     1381 ccgcaatggc tccgggcacg agccggagca tgccgcgcag gccagcgcga agcctcggga
     1441 gcgcgccgcg ccagcgcgcg cacagacgcg ccatggcgag ccgcgcccgc ctgctgccgg
     1501 caagccgcag cgcggggatc gcccgcagca cgggcgcggt gagcgccgcg gcaacgggga
     1561 ggcgcagccc agtcgccagg agagcaaggc gcgccagccc tgggaaccgc ggccaccccg
     1621 tccgcagcgc taccgggagg atcgcacacc ctcacccgtc ggcctcggcg accatgtgcc
     1681 ggccttcctg aaaaaggccc cccgtcccgc cacgcgctag agctggcggc gacgtggcga
     1741 ggatgatgtt ggcagggtgc ggcccgcacg gcgtgcgggc agcgggcccc tttcgctcac
     1801 gccaagtcct tcgggtcgct tagcccgggg ggcggtcgct gaaaaggaag ccacccttgg
     1861 ccttcgagtc cttgggggat tccatgattt gcaccatgac cgcgtccggc ttgacgttga
     1921 agtggcgcac caccgcgtcc gtaatatcct tcaccagcgc gcgcttctgc tcgatgctgc
     1981 ggccctcgat ggcgtgtacg tagacttccg gcataggcgg cctcctcgct agagcccggg
     2041 ccgttccggc ggtcggcgcc aggccggccc ggcactccga cgtactctaa cgcgggcccc
     2101 gccgcgcgtc atcgcccgct cttgcggccc tgcccagatc ggctattggt gaggacgacg
     2161 cacgacctca acggcggcgg caggcgccaa gatcgctgaa gggacgcgtc actggaggga
     2221 agcgccatgc tgcagctcgt gccaaacctg caaaggctgg ggaccgaaac ggccttcgaa
     2281 gttctggcgc gcgcggccga actgcagcgc caaggcaagg atgtgatcaa tctcggcatt
     2341 ggccagccgg acttccagac gccgccgcat atcgtcgagg ccgccgtcaa ggcgctgcgc
     2401 gacggccacc acggctacac gccggcgcag ggcatcttgc cgttgcgcga ggcggtggcg
     2461 gccgatttaa agcgccggca cggcgtcgat gtctccccgg acacgatcgt catcatgccc
     2521 ggcggcaagc ccacgatgtt ctttgccatc ctgatgttcg ggcaacccgg cgccgacatc
     2581 atgtatccgg accccggatt tcccatctat cgctcgatga tccagtacac cggtgcgcgg
     2641 ccggtgccga tcgcgctgtt ggaaaaaaac gagttcgcct tcacggccga ccaagtgctg
     2701 gcacagatca caccgcagac cagcctcatc attctcaata gcccgaacaa tccctgcggc
     2761 ggcgccgtgc ccgccgagga ggtcaagcgc ctcgtcatcg gccttgagaa gcacccgcac
     2821 gtcgccgtgc tgtcggacga gatctacagc caaatgctct acggcggacg caagcacgaa
     2881 agcttcctgc ggtatccctc gctgcgcgat cggctaatcg tgctcgacgg ctggtcgaag
     2941 acctacgcca tgaccggttg gcgcctcggt ttttcggttt ggccgcaggg cctggtcgag
     3001 cacgttgtgc gcctgtgcgt gaacaaccac tcttgcgtca acgcgccgac acagtacgcc
     3061 ggcatcgccg ccctcgaggg tccgcaggac gccgtcggga cgatggtggc cgccttcgac
     3121 gagcgccgcc gcaccatcgt gccgctgctg aaccagctgc cgggcatccg ctgtctcgat
     3181 cccggcgggg cgttctacgt gttcccgaac gtcaccggta cgggaatgac ggccaaggat
     3241 ctgcagaact tgatgctgga aaaggcgggc gtggccacgg tcgcgggcac cagtttcggc
     3301 atccatggcg aaggctacat tcgcttctcc tatgccaatt ccgtggagaa tattcgcgag
     3361 gccgtgcgcc gcctggcggc gctgctcagg gattgacttt tatggacttg cagatccgcg
     3421 ataggaccgc gctcgtcacg ggtgccagca tcggcatcgg ccgcggcgtg gcgctcggcc
     3481 tggcgcgcga gggggcgcgg ctggcgctgg ttgcccgccg gcgcgagctg ctggaggagg
     3541 ttgccgccga ggtgatggcc gccggcggca gccggccggt cctgatcagt gaggacctgc
     3601 tgcaggaggg cgcgccggcc aggatcgcca gggcggcgct cgccgggctc ggatccgtca
     3661 gcatcctggt caacaatgcg ggcggcagcc ggcccttcaa gctcgacgcc agcgaggaag
     3721 cgtggcacga ggccatgacg ctcaatttta cgcgtcagcg ccagctcacg cacagcctgc
     3781 tcgatacgat gatcagcgcc aagtggggtc gcatcatcac catgacgggc aagtcggagc
     3841 ccgagggcat caacggcgcc ttctcggcca aagccgccgt gcacgcatgg gccaaaggtc
     3901 tttcgcgcga ggtcggccga tacggcatca ccgtcaattg catcccccct ggccggatca
     3961 tgtccgagca gattcgccgc aactacccgc ccgaataccg gcaatggcag gccgagcacg
     4021 aaatacccgt cggccgctat ggcgagcccg aagacgtcgc caacctcgtg tgcttcttgg
     4081 cctccccctt ggcggcctac atcaccggca ccgtcattcc cgtcgacggt ggcttgcgcc
     4141 gttaccaatt ctaagaaaat gggtcgcttc gcggccgcgg cggcagcccg gccgggtcta
     4201 taatgaacgc ttcttaatgt cggggggcga cggggggcgc agggtaccgc cgttccctca
     4261 agaaccgatc acgagccggg cgagagccct cgaacggaga taaagccaaa tgaagatgcg
     4321 agcccgcttt gtggcccccg ccctgttggc ggcggcggcc attcccacca tcgtcctggc
     4381 ccagagccag cagccccaag acgcccagag ccgaccggag cggcaggccc agcggccgca
     4441 attgagccct gaggcgcggg cgcgcctgca ggaaggccgc ttcgccatga tcaaggaaac
     4501 gctgaaactc aacgacgccc agcgcaagct gtgggagccc gtcgaggcgc agttgcgcgc
     4561 ttccgcggcc gagcgccaaa agctgcgcga ggagcggcaa gcggcacgtc agcagaatag
     4621 ggcgcaaccg gccctgcccg agcggctcga ccgcgccagc gagcgcatgg ccaagcgggc
     4681 ggcgcgcatg aaagccttcg ccgaggtgtt caagcccttc tacgaatcgc tcgatgagga
     4741 gcagaaggcc gtggccggca tcgtgctgcg gcaggcgcgc ggtggcggcc atcgaggcca
     4801 tggccaccgc tgggcgatgc agcgctgagc cgacggaacg cggctgcccg ccgcgttcct
     4861 gtgctggatc ggacggccgc gtcctccctg cggcctccct gatcctaagc ctgggcgtct
     4921 ggataacccc catccaggcg ccctagtctt gcccaacgcc agccctggtt tttctacggg
     4981 gggggcccgc catcaaccgc gcacgatgac caccacttgc tggggtgcgc cggagctggc
     5041 gcccgcgtga tcggtgacga ccacactgaa gccggccgtc tttcccggcg ccccgctgtg
     5101 cgtgaagacg atgttgccgg cggcaagatc ggcttgcgag aactgggtca ccggttggcg
     5161 cgccgcactt gccagggcaa cgaagccgtt gcgtgcattg ccgacccgga aaatgaggtc
     5221 cgtcggcgca tcgtcggggt cgacggcgct caggtcctcg agttcgattt tgaccgaacg
     5281 gccgcgctcc accgtcacgg tgaagtcgcc ggccagcgcc ggcggcacgt tgcgcacctc
     5341 ggcgctgaga ttgccaccca cctccatggc gagcgcaccg tagtgcacgt tgccgctgac
     5401 ctccgcggaa ctgcggatgt tgatcaggtt cttgacgaag acgttgccct gcagcgtgcc
     5461 gctgacctcc gcggtctgag accgcagcgt gccaaagcag cggccaccgg catggatcac
     5521 ggcgctctcc gcggccacct tgccttcgac atagccgaag acttcgattt gacggcaatt
     5581 gcgaacttca cccttgatga tcgtgtcctc ctgcacgacc agcaggcctt tggtgtcggc
     5641 cacggctccc ccgttctcgt ttgtgcttcc cgccgcacgg ggcgcgcgcg cgcgctggtc
     5701 gcgcccggcg gcaggcgcca gtatgggctg gcgcggccgg ggcgtcgagg ctcgtgtc
//
LOCUS       DASTMT010000460         1858 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_1905200, whole genome shotgun sequence.
ACCESSION   DASTMT010000460 DASTMT010000000
VERSION     DASTMT010000460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 1858)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1858)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1858
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1905200"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1565
                     /gene="ftsH"
                     /locus_tag="VFR00_10700"
     CDS             <1..1565
                     /gene="ftsH"
                     /locus_tag="VFR00_10700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEU0159776.1"
                     /translation="FMRQMQSGGGRAMGFGKSKAKLLTERHGRVTFEDVAGVDEAKSD
                     LQEIVEFLRDPQKFQRLGGRIPRGALLVGPPGTGKTLIARAVAGEANVPFFTISGSDF
                     VEMFVGVGASRVRDMFDQAKKNAPCIIFIDEIDAVGRHRGAGLGGGNDEREQTLNQLL
                     VEMDGFEANEGIIILAATNRPDVLDPALLRPGRFDRQITVPNPDVAGREKILRVHMRK
                     VPLAPDVDPKVIARGTPGFSGADLANLVNEAALLAARRNKRLVTQAEFEDSKDKVMMG
                     AERRSMIMTEEEKLATAYHEGGHALVNLLVPGNDPLHKVTIIPRGRALGVTMSLPERD
                     KLSYSKQWCEARIATMFGGRVAEQLVYGEDHLNTGASSDIMQATELARRMVTEWGMSD
                     KLGPLRYNENQQEVFLGHAITQRQNMSEDTARLIDEEIRRIVTEGERKAREVLASNRD
                     KLEAITQALMEYETISGEEVNALMRGEKIVRANDDEESKGPAGPAVPAAGKMRPSRPE
                     PGPGGLEPQPQT"
BASE COUNT          344 a          582 c          632 g          300 t
ORIGIN      
        1 ttttcatgcg ccagatgcag tcgggtggcg gtcgcgccat gggctttggc aagtcgaagg
       61 ctaagctttt gaccgagcgg cacggccgcg tcacctttga ggacgtggct ggcgtcgatg
      121 aggccaagtc cgatctgcag gagattgtcg agtttcttcg cgatccgcag aaattccaac
      181 gccttggcgg acgcattccg cgcggcgcgc tgctggttgg cccgcccggc accggcaaaa
      241 cgctgatcgc gcgggccgtg gccggtgaag ccaacgtgcc cttcttcacc atttcggggt
      301 ccgatttcgt cgagatgttc gtgggcgtcg gcgcctcacg cgtgcgcgac atgttcgatc
      361 aggccaagaa aaacgcgccc tgcatcattt tcatcgacga gatcgacgcg gtcggtcgcc
      421 accgtggtgc cggtcttggc ggcggcaacg acgagcgcga gcagaccctc aaccagctgc
      481 tggtcgaaat ggacggtttc gaggccaacg agggcatcat tattctggcg gccaccaacc
      541 gtcccgacgt gctcgatcca gcgctgctgc gtcccggtcg gttcgaccgc caaattacgg
      601 tgcccaatcc cgacgtcgcc ggacgcgaga agatcctgcg cgtgcacatg cgcaaagtgc
      661 cgttggcccc cgacgtcgat cccaaggtca tcgcccgcgg cacgcccggt ttttccggcg
      721 ccgatctcgc caacctggtg aacgaggcgg cgctgttggc ggcgcggcgc aacaagcgcc
      781 tggtgacgca ggccgagttt gaggacagca aggacaaggt gatgatgggg gcggaacgcc
      841 gctccatgat catgaccgaa gaggaaaagc ttgccaccgc ctatcacgaa ggcggccacg
      901 cgctcgtcaa tctgctggtg ccgggcaacg atcccctgca caaggtcacc atcattccgc
      961 gtgggcgcgc cctcggcgtg accatgagcc tgcccgagcg cgacaagctc tcctactcca
     1021 agcagtggtg tgaggcgcgc atcgccacca tgttcggcgg cagagtggcc gagcagctcg
     1081 tgtacggcga ggaccacctc aacacgggcg cctccagcga catcatgcag gcgaccgaac
     1141 tcgcccgacg catggtgacc gagtggggca tgagcgacaa actgggtccc ctgcgctaca
     1201 acgagaacca gcaagaggta tttctcggtc acgccattac gcagcgccag aacatgtccg
     1261 aggacaccgc caggctgatc gacgaagaga tccgccgcat tgtcacggaa ggcgagcgca
     1321 aggcgcgcga ggtgctcgcc agcaaccgcg acaagctcga ggcgatcacc caggcgttga
     1381 tggaatacga gaccatcagc ggcgaggaag tgaacgccct gatgcgcggt gaaaagatcg
     1441 tgcgtgcaaa cgacgacgag gagtcgaaag ggccggctgg gccggcggtg ccggcggccg
     1501 gcaagatgcg tccgtcgcgg ccggagccgg gccctggggg attggagccg cagccgcaaa
     1561 cttagcgcgg gttggcgggg agacccgaat tggggccgcg ccgcccaagg cgccttcctt
     1621 tcgtcctttt tgcctcgctt tgttggtatc aagcgtgggg ttgcgtatga cgcgccgctg
     1681 agtgcggcca gttctttgct tgtcgctcgt ggggcgacag ctcggcgcca catgatcgag
     1741 ggagcgtcgt gaagaggacg ggcgggcaag ggggcaggcg ggtggtggaa accacgctcc
     1801 gcccaaccac acgcacgttg accaggcggc cgccggcggc ggtgcagccc tggcgcgg
//
LOCUS       DASTMT010000461         4013 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_3218603, whole genome shotgun sequence.
ACCESSION   DASTMT010000461 DASTMT010000000
VERSION     DASTMT010000461.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4013
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3218603"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..378)
                     /locus_tag="VFR00_10705"
     CDS             complement(<1..378)
                     /locus_tag="VFR00_10705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012322130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEU0159777.1"
                     /translation="MEQIPILKVGRCLLVSIQIDMHDKLALALQDDLTQRLVETRARG
                     VLIDISALDLVDSFIGRMLSNIAAMARVLDAQTVVVGMRPQVAITLVELGLTLTGIQT
                     ALDVDRGLALINRLIDADGDDGAG"
     gene            complement(388..1257)
                     /locus_tag="VFR00_10710"
     CDS             complement(388..1257)
                     /locus_tag="VFR00_10710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018409416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEU0159778.1"
                     /translation="MAVSSISRILDLTDKHHSRLLTSWLESQVAAGSLRSGQIREDEL
                     KDQSHRFLQEFIDGLKSGDVDDIMGPRWSGARELLGEFSRSRALQGFTPSETATFIFS
                     LKEPLFGLMREELKGSAQQLGEAIWTATKVIDKLGLYTAEVYQQSRDEIIRRQAHELL
                     ELSTPVVELWDKVLALPLIGTLDSERTQTVMENLLNHIVKSGAEIAIIDITGVPTVDT
                     LVAQHLIKTVSAARLMGADCIISGIRPQIAQTIVHLGLQLDVVSKATMADAFALALKR
                     TGQFIGRVAAKPA"
     gene            complement(1545..1754)
                     /locus_tag="VFR00_10715"
     CDS             complement(1545..1754)
                     /locus_tag="VFR00_10715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0159779.1"
                     /translation="MKRDPVSHERGRPPETEAWKIIFGAFAVSGSLVIGWTLFVLLTD
                     SPSRAPRLGEQASQTTDRPAAPAKP"
     gene            2014..3630
                     /locus_tag="VFR00_10720"
     CDS             2014..3630
                     /locus_tag="VFR00_10720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012042402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HEU0159780.1"
                     /translation="MPITRPDLEADYAVIGAGSAGCVLANRLSQGGARVVLLEAGPRD
                     WHPMIHVPAGVLKLLYNPRVNWNYATDPEPGAGGRQIHWPRGRVLGGSSSINGMLWIR
                     GNAADYDGWAQMGCKGWSFDELMPYFKSIERYAGGAAAERGKSGPILVEDYRTTLALT
                     HRFVDAALQAGLPLTQDLNGSQQEGVGYSQMSRNGRFRGSTARTFLAEAKGRANLSIE
                     TNAFATRLLCDHKRCTGVVFRQGGRERRLVVAREVILSGGTINSPHLLQVSGIGPAAH
                     LQAIGVPVVHDLPGVGANLSDHYAIRISHRVKNAVSINELSRGLRLAGEVARWLTVGR
                     GALTFGVSSAQAFARSRQGLASPDIQLLFSPASYDANKFGALEREPGMTIAVSIARPE
                     SRGSIMAKSPDPFERPSLKPNYLSVANDLRVALRGIALARRVFAAPALAQHSVREITP
                     GSDISGEDGLAAYVRAQGTTIYHIVGTCKMGEDPMAVVDSRLKVRGLSGLRVIDASVM
                     PTVTTANTNAPTIMIAEKGAAMVLADARLS"
     gene            3655..>4013
                     /locus_tag="VFR00_10725"
     CDS             3655..>4013
                     /locus_tag="VFR00_10725"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015686270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD-dependent protein deacetylase"
                     /protein_id="HEU0159781.1"
                     /translation="MAKVEELQRMIAGAGRLVAFTGAGISTESGIPDYRSPGGIWSKF
                     RPIEFGDFLASAEARRETWRRKFASHEVIEKATPNAGHRALARLVEQGKMLAIITQNI
                     DGLHQASGVADDKVIEL"
BASE COUNT          692 a         1320 c         1311 g          690 t
ORIGIN      
        1 acctgcaccg tcgtctccat ccgcatcaat cagccgattg atgagggcca gtccgcggtc
       61 gacatcaagc gctgtctgaa tgcccgttag cgtcagcccg agttcgacca gcgtgatggc
      121 tacctgcggc cgcatgccaa ccaccaccgt ctgtgcgtcg agcacgcgcg ccatggcggc
      181 gatgttcgac agcatgcggc caataaagct gtcgaccaga tcgagcgcag agatgtcgat
      241 caaaacgccg cgggcgcggg tctcgaccag tcgttgcgtc aggtcatcct gcagcgccag
      301 cgccaatttg tcgtgcatat cgatttgaat gctgaccagc aggcaccgcc cgacctttag
      361 gatcggtatt tgttccatcg cggcgcctca ggctggcttg gccgccacgc ggccgatgaa
      421 ctggcctgtg cgtttcaggg ccaaggcgaa ggcgtcggcc atggtcgcct tggagacgac
      481 gtcgagctgc aacccaagat gcacgatggt ctgcgcgatc tgcggacgga tcccgctaat
      541 aatgcagtcg gcacccatca gcctggcggc cgatacggtc ttgatcagat gctgcgcgac
      601 cagggtatcg accgtcggca ccccggtaat gtcgatgatg gcgatctccg cgcccgactt
      661 cacaatatga ttgagcaagt tttccatcac cgtttgcgtg cgctcgctat cgagcgtgcc
      721 gatcaacggc agggcaagaa ccttatccca cagctcgacg accggcgtcg aaagctccaa
      781 cagctcgtgc gcttggcgcc tgatgatttc gtcgcggctt tgctggtaga cctcggccgt
      841 gtaaagaccg agcttgtcga tcaccttggt ggcggtccag atcgcctccc ccaattgttg
      901 ggcactgccc ttcaattcct cgcgcatcaa gccgaacagc ggctctttca gggaaaagat
      961 gaaagtcgcc gtttccgagg gggtgaaacc ctgtagcgcg cgcgagcggg agaattcgcc
     1021 gagcagctcg cgggcgccgc tccagcgcgg tcccatgatg tcgtcgacgt cgccggattt
     1081 caggccgtcg atgaattctt gcaagaagcg atggctctgg tccttgagct cgtcttccct
     1141 gatttggccg gagcgcagcg acccggcggc cacctggctc tccaaccagg acgtcaagag
     1201 cctggagtga tgcttgtcgg tcaaatcgag aatgcggctg atgctgctga cggccattcg
     1261 cgtatccagg gtagggagca ggggcgttgg ttagcattga ggcgggggcc gagcaaccgc
     1321 cccacccccg ctcggtaaac gcggcagaag gcgcaaaagt ccttgtgcgg gcaaacgcgg
     1381 tggcccggct cgagcgccgg cccccactcg accgcgtcgg ggttagcccg gcccggcaag
     1441 ccacggcgtc gacccgcggc aaggccggcc aagtggcggg cgcaacgctg caagcaccct
     1501 ggcgtttcag cgccctgggc ggactgtccg gtctggccgg gaggctaggg cttggcgggg
     1561 gctgcggggc gatcggtcgt ttggctcgcc tgctctccca ggcggggcgc ccgtgacggg
     1621 ctgtcggtca agagcacgaa cagcgtccat ccgatcacca gcgagccgct gaccgcgaac
     1681 gccccgaaaa tgatcttcca ggcctccgtt tccggaggac ggccacgctc atgacttact
     1741 gggtctcgtt tcatgcgtcg caagcctttc ctcccaaaaa cccgcaagcg gcgccgcctg
     1801 ttcctgcggg gcaagccacc tgcgcccgcc gcggcgaagc tggcgatgct ggccagcggg
     1861 aagttgcctg gccctgccgc ggtgctgcgc cgaagcggtg ccgccctgcg cgccacggcc
     1921 tgcggcccct cgcaatagcg cgccgcccct tgtggttttt cgctaactcg ccgacgatcg
     1981 caacggacgt ggcacgaggg aggaggcgac aaattgccaa tcacgaggcc tgacctggag
     2041 gcggactatg ccgtgatcgg cgccggctcg gcgggctgcg tgctcgccaa ccggctcagc
     2101 caaggggggg cccgcgtggt gctgctcgag gccggcccca gggactggca tccgatgatc
     2161 cacgttcccg ccggggtcct gaaactcctc tacaacccgc gggtgaactg gaactatgcc
     2221 accgatccgg agccgggcgc cggcggccgg cagatccatt ggccacgcgg gcgcgtcctc
     2281 ggcggctcca gctcgatcaa cggcatgctt tggatccgag gcaatgcggc tgactacgac
     2341 ggctgggcgc agatgggctg caagggatgg tccttcgacg agctcatgcc ctatttcaag
     2401 tcgatcgagc gctacgcggg cggcgccgct gccgagcgcg gcaagagcgg accgatcctg
     2461 gtcgaggact accgcacgac gctcgccctg acgcatcggt tcgtcgacgc agcgctgcaa
     2521 gccggtctcc cgctcacgca ggacctcaac ggcagccagc aagagggggt cggctactcg
     2581 cagatgtcgc gcaacggccg ctttcgcggc tccaccgccc gcacgttcct ggccgaggcg
     2641 aagggcagag ccaatctgtc catcgagacc aatgccttcg cgacgcgcct gctctgcgat
     2701 cacaagcgct gcacgggggt cgtgttccgc cagggcgggc gggagcgccg cctggtagtg
     2761 gcccgcgagg tgatcctctc cggcggcacc atcaactcgc cgcacctgct gcaggtttcg
     2821 ggtatcgggc cggccgcgca tttgcaggcg atcggggtgc cggtcgtgca cgacctgccg
     2881 ggggtcggtg ccaatctgtc cgaccactat gccatccgta tttcgcatcg cgtaaaaaac
     2941 gcggtttcga tcaatgaatt gtcgcgcggg ctgcggcttg cgggcgaggt cgcgcgctgg
     3001 ctcacggtcg gacgcggcgc cttgaccttc ggcgtctcca gcgcccaggc cttcgcgcgc
     3061 agccggcagg ggctggcctc tccggacatt cagctgttgt tctcacccgc gagctacgat
     3121 gccaacaaat tcggggcgct cgaacgcgag cctggcatga cgattgccgt cagtatcgcg
     3181 cggcccgaga gccgcggcag catcatggcc aagtcgccgg accccttcga acgcccatcc
     3241 ttgaaaccga attacctgtc ggtggccaac gacttgcggg tggcgctacg cggtatcgcg
     3301 cttgcccgtc gcgtatttgc cgcgccggcg ctggctcaac atagcgtgcg ggagatcacg
     3361 cccgggtccg acatcagtgg cgaggacggc ctggccgcct acgtgcgcgc gcaaggtacg
     3421 accatctatc acatcgtggg cacctgcaag atgggcgaag acccgatggc ggtggtcgat
     3481 tcccgcctca aggtgcgcgg gctcagcggc ttgcgggtga ttgacgcctc cgtcatgcca
     3541 acggtgacga ccgccaatac caacgcaccc accatcatga ttgccgagaa gggcgcggcg
     3601 atggtgctgg ccgacgcccg cctcagctag caggcaaaga gcgagggtcc gggcatggca
     3661 aaagtcgagg agctgcagcg catgatcgcc ggcgctgggc ggctggtggc cttcaccggt
     3721 gctggtattt ccaccgaatc gggcattccc gactaccgct cccccggcgg catttggagc
     3781 aagttcagac ccatcgaatt tggcgatttt cttgcgtctg cggaagcccg ccgcgaaacc
     3841 tggcggcgca agttcgccag ccacgaggtg atcgaaaagg cgacgccgaa cgccgggcac
     3901 cgcgcactcg cccgtctggt cgagcaaggc aagatgttgg cgatcatcac gcagaatatc
     3961 gacggcttgc atcaggcctc gggcgtagcc gacgacaagg tcatcgagct gca
//
LOCUS       DASTMT010000462         1939 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_5711845, whole genome shotgun sequence.
ACCESSION   DASTMT010000462 DASTMT010000000
VERSION     DASTMT010000462.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 1939)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1939)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1939
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5711845"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1256
                     /locus_tag="VFR00_10730"
     CDS             <1..1256
                     /locus_tag="VFR00_10730"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEU0159782.1"
                     /translation="KDLSDTEGLLTSHGSPLYRDHVPTADAAMVALLRKAGAIIVAKT
                     NTPEFGAGANTRNAVWGATGNPFDPQLNAGGSSGGSAAALACDMLALCTGSDMGGSLR
                     IPAAKCGVVGMRPSPGLVPYDGRPLGWTPLAVVGPMGRTVADLRLLFAAQISVDDREP
                     LAFALDADAIARARVVDLGRLRVAWTEDFAQCPVSAEIRRVMRARIAAMRHLFRSCDE
                     IAFDFAEADRCFDIVRAQSFVARHQDAYQADPASLGPNVRANYELGAKLTLSDAAWAH
                     AEQTRIFHRFQAHFRDYDLVLSPTTPVSPFPWSSLYLAELEGRPLRNYYHWLALTYYI
                     SLVTNPAISLPCGVDEHGMPFGVQVVGRFRGDVELLNAAEAMEAAFSRIGGLGRPRPD
                     LTKLRGTTTPALKSIVTHPPLLSTH"
     gene            1275..>1939
                     /locus_tag="VFR00_10735"
     CDS             1275..>1939
                     /locus_tag="VFR00_10735"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEU0159783.1"
                     /translation="MNGKRPTMTSLHGMVAAAHPLAAQAGAKLLSAGGNAFDAAAATA
                     AALNVVEPFMSGLAGLGYATCYIAAEQRVRVLDFVPPVPSRLPVERFSRREDLQRGAL
                     SVGTPGNLAGWAELVRAYGKKPIAAALAPAIALARDGFALAEFGVAEFNEQIPLLRRC
                     TAFYNDWAHAYTGGATSMALGRIVRQPELAATLEAIAAEGWRHLYGGSLGEMIVSHLQ
                     RLGG"
BASE COUNT          290 a          670 c          609 g          370 t
ORIGIN      
        1 tcaaggactt gagcgacacg gagggtctgc tcaccagcca cggctcgccg ctctaccgcg
       61 atcacgtgcc gacagccgac gccgctatgg tcgcgctcct gcgcaaggcc ggcgccatta
      121 tcgttgccaa gaccaacacc cccgagttcg gcgctggcgc caatacgcgc aatgccgtgt
      181 ggggtgcgac cggcaatccc ttcgaccctc agctgaatgc gggtggttcc tcgggtggtt
      241 cggcggcggc tctggcctgc gacatgcttg cgctctgtac cggctcggat atgggtggtt
      301 cgctgcgcat tcctgccgcc aaatgcgggg tcgtcgggat gcgaccctct cccggcctcg
      361 tcccctacga cgggcgcccg ctcggctgga cgccattggc cgtagtcggg ccgatgggcc
      421 gaacggtggc agatctgcgc ctgctctttg ccgcgcaaat aagcgtcgac gaccgcgaac
      481 ccttggcctt tgccctcgat gccgacgcta tcgcccgcgc ccgggtcgtc gatcttggcc
      541 gcctgcgcgt tgcctggacc gaggatttcg cgcaatgccc ggtatcggcc gagatcagac
      601 gcgtgatgcg cgcccgcatt gcagccatgc gacacttgtt ccgctcgtgt gacgagattg
      661 ccttcgattt tgccgaggcg gatcgctgtt tcgacatcgt gcgtgcccag agcttcgttg
      721 cacgacatca ggatgcctac caggccgacc ccgcctcgct tggccccaat gtgcgcgcca
      781 actatgagct gggtgccaag ctgacgctat ccgatgccgc ctgggcacac gccgagcaga
      841 cgcgcatttt tcatcgcttc caagcgcact ttcgtgacta cgatttggtg ttgtcgccga
      901 cgacgcccgt gtcgcccttt ccctggagct cgctctacct tgccgagctc gaaggccgcc
      961 ccctgcgcaa ctactatcat tggctggcac tcacctacta catcagcctt gtcaccaatc
     1021 cggcgatttc gttaccgtgc ggcgtcgacg agcatggcat gccgttcggc gtgcaggtcg
     1081 tcggccgttt tcgcggcgac gtcgagcttt tgaatgcggc cgaggccatg gaggctgcct
     1141 tctcccgcat cggcgggctc ggccggccgc ggccggatct aacaaaactt cgcggcacga
     1201 ccacgccggc gctgaaatcg attgtcaccc acccacccct cctttccact cattgacgat
     1261 tggctcgcac aaccatgaat ggcaaacgac cgacgatgac ctcgctgcac ggcatggtgg
     1321 cggccgcaca cccgctggct gcccaggccg gcgccaagct gttgagcgcg ggcggcaatg
     1381 ccttcgacgc cgcggcggca acggcggcgg cacttaacgt ggtcgagcca ttcatgtctg
     1441 ggcttgccgg cctcggctac gccacctgct acatcgctgc cgagcagcgg gtccgcgtgc
     1501 ttgacttcgt gccgccagtg ccttcgcgcc tgcccgtcga gcgcttcagc aggcgggagg
     1561 acctgcagcg cggcgccctg tcggtcggga cgcccggcaa cctggcggga tgggcggagc
     1621 tcgtgcgcgc ctatggcaaa aagccgattg ccgcggccct ggcaccggcg attgctttgg
     1681 cccgcgacgg cttcgccctc gccgaattcg gcgtggccga gttcaacgaa cagatccccc
     1741 tgctgcgccg ctgcacggcc ttttataacg attgggctca cgcctatacc ggtggcgcga
     1801 caagcatggc gctggggcgc atcgtgcgcc agccagagct cgcggcaaca ctcgaggcca
     1861 tcgcggctga gggttggcgc cacctttatg gcggcagttt gggggaaatg attgtctcgc
     1921 atctgcagcg gctcggcgg
//
LOCUS       DASTMT010000463         4131 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_2557497, whole genome shotgun sequence.
ACCESSION   DASTMT010000463 DASTMT010000000
VERSION     DASTMT010000463.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4131)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4131)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4131
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2557497"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..810
                     /locus_tag="VFR00_10740"
     CDS             <1..810
                     /locus_tag="VFR00_10740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018262061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU0159784.1"
                     /translation="HMSVYDNMAYGLRNRGVPKADIAARVAEAARLLAIEDFLARKPR
                     ALSGGQRQRVAMGRAIVREPQAFLFDEPLSNLDAKLRVQMRVEIRRLHNRLKATSIFV
                     THDQVEAMTLADLVVVMNAGRIEQTGGPTEVYRQPATRFVATFIGSPAMNLLPGRITG
                     PGTVEIKGGRMPFRAEAFAFAVGQEVDVGVRPEDLVLSRAGAVNGLPFARDFVEELGA
                     TRLIHGNLGEVPIVVAVATSAEAVADGAIAVEPAAVHLFDRASGSSLRRGA"
     gene            complement(861..1760)
                     /locus_tag="VFR00_10745"
     CDS             complement(861..1760)
                     /locus_tag="VFR00_10745"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU0159785.1"
                     /translation="MRRLCEGRVAIVTGAGGGLGRQHALLLAAHGAKVMVNDIGAGVD
                     GRGVDASVADAVVGAIKAAGGEAIANGADVSDWHGAQAMIDAAVGTFGRLDILVNNAG
                     ILRDRMLTNMAEDEWDAVIKVHLKGTFAPSRHAAAYWREESKKANAPVKGRIINTTST
                     SGIYGNVGQTNYGAAKAGIAAFTVIAARELRRYGVTVNAISPSAQTRMTEGLRELSAD
                     ERAARDPKWVSPTVVYLASEEAQDITGRVVQAGFGRLAVCEGWRRGAEVGQVEEPAGA
                     GRLLREMIARVRKNSGMDGLELD"
     gene            1949..2689
                     /locus_tag="VFR00_10750"
     CDS             1949..2689
                     /locus_tag="VFR00_10750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0159786.1"
                     /translation="MLNDRLDGIAARLLAARREGARVALAASETPQDFEQGFAIQDRV
                     VAALASAVIGWKVMPVANGPVIYAPILQSGQVRAGGTWAVVGGEPAGLELEIAFRLSR
                     SVPPEADAAEILQAVAAAHVVFELCQSRIADPAKQPRHVMLADCIANAGIVVGSEIPD
                     WRRRDLKARPGRLFVDGALYAEGVSLDPVAALHMLPAAMAHRGQRLEAGQIVITGSLI
                     GMNWLTGRHELKGVIDDCGEVAVALAAA"
     gene            complement(2771..3730)
                     /locus_tag="VFR00_10755"
     CDS             complement(2771..3730)
                     /locus_tag="VFR00_10755"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018698878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU0159787.1"
                     /translation="MAVNAHRVGWIGMGRMGYPMAERLLKAGHDVAIWNRTAAKAQPL
                     AAAGAKVVNTPADLGGVDVLFSIVSTGKDLEQVYFGPAGALSVAGRVPAIVVDCSTIG
                     VEESERIRAKLREAGADYIAAPVSGNAKVIKAGKLSAVASGPEPAFRKVAPLIEAFAP
                     GGVSYVGEGELARVCKIAHNVMLGVVIENLIEITLLANKMGVPRHAFLAFMNNSVMGS
                     MFTRYKSPALVNLDWTTTFTPELLRKDLDLGLDLARKMEVPMPVTSAAREVLQSHIGA
                     VHLKPDAEHYLAADFAALLETLAVASGMRLESEQKTVPTGLEG"
     gene            complement(3834..>4131)
                     /locus_tag="VFR00_10760"
     CDS             complement(3834..>4131)
                     /locus_tag="VFR00_10760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008967928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0159788.1"
                     /translation="LASQRATLDFALDLLAVAALPRTTVQSPLRWSSSPDWKLDFSNV
                     QRLSAAEIARRRAEFDKQKAIARGVREDAGVTRDTLKPQGPAGAAASRDRSLTG"
BASE COUNT          647 a         1408 c         1364 g          712 t
ORIGIN      
        1 cacatgagcg tctacgacaa catggcctac gggctgcgca atcgcggcgt gcccaaggcc
       61 gacattgccg cgcgcgtggc cgaggccgcg cgcttgcttg ccatcgagga tttcctcgcc
      121 cgcaagccgc gtgcgctttc ggggggccaa aggcagcgcg tcgccatggg tcgagcgatc
      181 gtgcgtgaac cgcaggcttt cctgttcgat gaacccctgt ccaatctcga cgccaagctg
      241 cgcgtgcaga tgcgggttga gatccggcgc ctgcacaaca ggctcaaggc aacctccatc
      301 ttcgttacgc acgatcaagt cgaagccatg acgctcgccg acctggtggt ggtgatgaat
      361 gccggtcgta tcgagcagac cggcgggccg accgaggtct atcgccagcc ggcgacgcgc
      421 ttcgtggcga ccttcatcgg ctcgcccgcc atgaacctgc tgccgggccg cattaccggc
      481 cccggcaccg tcgagatcaa gggcggacgc atgcccttcc gcgccgaggc cttcgccttc
      541 gccgtcggcc aggaggtgga tgtgggcgtc cgtcccgagg atctggtcct cagccgcgcc
      601 ggtgccgtca acggcctccc ctttgcccgg gatttcgtcg aggaattggg cgctacgcgg
      661 ctcatccacg gcaacctcgg cgaggttccg atcgttgtgg cggtggcgac ctccgcggaa
      721 gccgtcgccg atggcgccat tgccgtcgag ccggcggcgg tgcacctgtt cgacagggca
      781 agcggcagca gcctgcggcg cggcgcctga gatcttgcgc agagcccgcc ctgcgggccg
      841 ctcggcgctg gttcggcgcg tcaatcgagc tctagaccgt ccattcccga attcttgcgc
      901 acgcgcgcga tcatctcgcg caagagccgc cccgcgccgg caggctcctc tacctggccg
      961 acctcggccc cgcgccgcca gccctcgcac acggcaagac gaccaaagcc ggcctggacc
     1021 acccggccgg tgatgtcttg ggcctcttcg ctcgccagat agacgacggt gggggagacc
     1081 cacttggggt cgcgcgcggc gcgctcatcg gcgctgagct cacgcaagcc ttcggtcatc
     1141 cgcgtctggg ccgacggtga aatggcattg acggtgacgc cataacggcg cagctcgcgt
     1201 gcggcgatga cggtgaaagc ggcaatcccg gctttggcgg ccccgtaatt cgtctggcca
     1261 acattgccgt aaatgcccga ggtcgacgtt gtgttgatga tgcgtccctt gaccggggcg
     1321 ttggccttct tgctctcctc gcgccaatag gcggcggcgt gccgtgacgg tgcaaatgtc
     1381 cctttcagat gcaccttgat gacggcatcc cactcgtcct ccgccatgtt cgtcagcatg
     1441 cgatcgcgca ggatacccgc attgttgacg agaatgtcga gcctgccaaa ggtgccgacg
     1501 gcggcatcga tcatcgcctg cgcgccatgc caatcgctca cgtcggcccc attggcaatc
     1561 gcctcgccac cggccgcctt gatcgcgccc accaccgcat cggcgaccga ggcgtcaacg
     1621 ccgcgcccat cgacgccggc gccaatatcg ttgaccatga ccttggcgcc gtgcgccgcc
     1681 agcaggaggg cgtgctggcg ccccagcccg ccgccggcgc cggtgacgat ggcgacccga
     1741 ccttcacaca aacgccgcat acccttctcc tcgtgttgac cgatagcgca tcagctcggt
     1801 cggcgttgac gacaacaggc aagcccgcgg cggcgagccc tgggggacgt gcgggaatgt
     1861 ctttcaactc ccgccacgcg catgcgatac ccctcctgcg gccgccggcc ggggccggct
     1921 gcgcagaagg ctagggcagg agaactcgat gctcaacgat cgtctcgacg gcattgccgc
     1981 ccgcttgctg gccgcccgcc gcgagggggc ccgtgttgcg cttgccgcct cggagacgcc
     2041 gcaggacttc gagcaaggtt tcgccatcca ggacagggtc gtcgccgctc tggcctctgc
     2101 cgtgatcggt tggaaggtga tgcccgtggc gaacggtccc gtgatctacg cgcccatcct
     2161 gcaatccggc caggtgcgcg cgggcggcac ctgggcggtg gtgggagggg aacctgcggg
     2221 ccttgagctc gagattgcct ttcgcctgtc ccgcagcgta ccgcctgagg ccgacgctgc
     2281 ggagatcttg caggcggttg cggccgccca cgttgtcttc gagctgtgcc agagccgcat
     2341 cgccgatccg gccaagcagc cgcgccacgt catgctggcg gactgtattg ccaatgccgg
     2401 cattgtcgtc ggttcggaaa tccccgactg gcgcaggcgc gacctcaagg cgcggccggg
     2461 ccggctgttc gtcgatggcg ccctctacgc ggaaggtgtg agcctcgacc ccgtggccgc
     2521 cttgcacatg ttgccggccg ccatggccca ccgcggccag cgcctggagg ccgggcagat
     2581 cgtcatcacg ggcagcctga tcggtatgaa ctggctgacc ggccgacacg agctcaaagg
     2641 cgtgatcgat gactgcggcg aggtggcggt cgcgctcgcc gcggcctgag cccgtcgggc
     2701 ggtggcgacg ccgcggggct tggcgtgcgg tcgtacgcgg agcgagccgc gcccagtcac
     2761 gcttgcgcgc ttaaccctcg agcccggtcg ggaccgtttt ttgttcgctc tcgaggcgca
     2821 tacccgaagc caccgccagc gtctccagca atgccgcgaa atcggcggca agatagtgtt
     2881 cggcatcggg cttcaaatgc acggcgccga tgtggctctg caaaacctcc cgcgctgccg
     2941 atgtcacggg catcggcact tccatcttgc gcgccaagtc caggccgagg tcgagatcct
     3001 tgcgcaagag ctcgggcgtg aaggtcgtcg tccagtcgag gttgacgagc gccggcgact
     3061 tgtagcgcgt gaacatcgaa cccatcacgc tgttgttcat gaatgccaga aaggcgtgcc
     3121 gtggcacccc catcttgttg gccagcagcg tgatttcgat cagattctca atgacgacgc
     3181 cgagcatcac gttgtgggcg atcttgcaca ctcgcgccaa ttctccctcg cccacatagg
     3241 aaaccccgcc cggggcgaag gcttcgatca gcggcgccac cttgcgaaag gcgggctccg
     3301 gaccggaggc caccgcggaa agcttgccgg ccttgatgac tttggcattg ccgctgacgg
     3361 gggcggcgat gtagtcggct cccgcttccc gcaattttgc gcggattcgt tcggattcct
     3421 cgacgccgat ggtcgagcag tcgacgacga tggcagggac cctgccggca acggagagcg
     3481 cgccggcggg gccgaagtaa acctgctcca ggtctttgcc ggtcgagaca atcgaaaaca
     3541 acacgtcgac gccgccaaga tcggctggcg tattgaccac ctttgcgccg gccgccgcca
     3601 gcggttgggc cttggcggcg gttcggttcc agatcgccac gtcgtggccg gccttgagca
     3661 ggcgctccgc catcgggtag cccatgcggc ccatgccgat ccagccgacg cggtgcgcgt
     3721 tcactgccat tgcggtctcc tcgaagaatg ccggtggttg gatgtggatg aggtgcccgc
     3781 cccggccggc ggcggcttgc gctttaaagg taggcaattg agcccgtgcc gcgtcagcct
     3841 gtcaacgagc gatccctcga cgctgccgcg ccggcggggc cctgcggctt gagggtgtca
     3901 cgcgtgacgc cggcgtcttc acgcacaccc cgggcaatgg ccttctgctt gtcgaattcg
     3961 gcgcggcgtc gcgcgatttc cgccgccgac aagcgttgaa cattggaaaa gtcaagtttc
     4021 caatcggggc tagagctcca tcgcaacggc gattgcaccg tcgttcgcgg cagggccgcg
     4081 accgcgagca aatcgagggc aaaatcgagc gtggcgcgct gggatgctaa a
//
LOCUS       DASTMT010000464         2532 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_6043006, whole genome shotgun sequence.
ACCESSION   DASTMT010000464 DASTMT010000000
VERSION     DASTMT010000464.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2532)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2532
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6043006"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..402)
                     /locus_tag="VFR00_10765"
     CDS             complement(<1..402)
                     /locus_tag="VFR00_10765"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEU0159789.1"
                     /translation="MKLMMFDRGNGATLGLVENHAVVDLAAADPTLPKDLNGLMLGGT
                     RALTAAKAAAAAQPASARLALDAVTALLPIQPAAKIICVGLNYALHAKEGGHPIPTYP
                     SFFLRVYSSLLAADAPVVRPKLSAQLDYECEL"
     gene            968..>2532
                     /locus_tag="VFR00_10770"
     CDS             968..>2532
                     /locus_tag="VFR00_10770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014440068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HEU0159790.1"
                     /translation="MYDFIIVGGGSAGSVLANRLSARSTCEVLLLEAGQDTPHGRVPP
                     EVLDSYPGTAYFDPRYHWTELKVRTEVVSHNNPTESPPPLRKYEQARILGGGSSINGQ
                     LANRGAPADFEEWVARGARGWGWKDVLPYYKKVERDMDFDGALHGKEGRIPVRRIFPD
                     SWTGHAKAAAEAFKGAGFEYLPDQNGEWRDGYYPIAISNAYERRVSAAIGYLDPATRQ
                     RNNLRIATDTQVSELTFEGQRCTGVVANVKGQRREFKAREVILSCGAIHSPAHLLRAG
                     IGPAGHLKDLGLEVRHASPGVGQGLMDHPSISVSAYLKPHGRMNELTRRHIHVGLRYS
                     SDMPGMHEGDMFVVAISKSAWHAVGERIASFLIAVYHTASERGEVKLASRDWRDEPRV
                     EFNLLSDRRDLERLMQAFRKMGRLYGTAALRDVTSDPFPATYSDRVRKVGVVNTKNKL
                     ITSIAGTLLDGPQALRSYLIRTLITEGFGFDQVMQDDDALEAFVRKAAIGVWHASCSA
                     RMGAEGDSMAVTD"
BASE COUNT          436 a          822 c          830 g          444 t
ORIGIN      
        1 cagctcgcac tcgtaatcga gttgggccga cagcttgggc cgcaccaccg gcgcgtcggc
       61 cgccagcaag gagctgtaca cgcgtaggaa aaacgaggga taggtgggaa tggggtgccc
      121 tccttccttg gcgtgcagcg catagttgag cccgacgcag ataattttcg ccgcgggctg
      181 gatcggcagc agggcggtga ccgcgtcgag cgccagcctg gcggaagcgg gttgcgcagc
      241 cgccgcggcc ttggcggcgg tgagcgcccg ggttccaccc agcatcaggc cattgagatc
      301 ctttggtagg gtgggatcgg cggcagcaag atccacaacg gcatggttct ccaccaagcc
      361 aagggtcgcg ccattgcctc tgtcgaacat catcagcttc attgccgcct ctccttcagg
      421 tttcacccga gcttgccggc cgatcgacgc gaagacgctc aatcgcccac gtcacttgag
      481 cgcggccaga gccgccttcc gtgcgccccg acaagacccc ctcgttgagc gcaacgagga
      541 tgtctcgcct cagacgtcgc cacctttttt agcgatctcg cgcgtccttc gaaagagcgg
      601 ctgccgccgc gtttgctcct ccggcagtga tttggcgcta tggccatgcg ccgggccgcc
      661 ctcggagaga acaatagggc gacgccccga gtcgccgcgc gacaggtgcc tgggccggct
      721 ggtgcgcacg cgcagcatgg cagcgacccc ggcatggcat cgacccagca tccgaccgcc
      781 gctcgcgccg gccggcctgg cggggctgca atgctgcccg ggggcgcgca ggccgttgcg
      841 ggcttgacag atcggtcttt aagctgctgc ccttcacgtc tcgccgctcc agccgcccac
      901 ccctcgcatg aagccggagg atcgccagcg agtggcttcg ttgtccgcgc agcagaggga
      961 aagcatgatc tacgatttca tcattgtcgg cggcggctcg gccggctcag tgctggccaa
     1021 ccggctatcg gcgaggagca catgcgaagt tttgctgctc gaagccggcc aggacacccc
     1081 gcacggtcgc gtgccgcccg aggtgctcga cagctatccc ggcaccgcct acttcgatcc
     1141 gcgctaccat tggacggagt tgaaggtgcg caccgaggtc gtatcgcaca acaatccgac
     1201 ggaaagcccg ccacccctgc gcaagtacga gcaggcccgg atcctaggtg gcggttcctc
     1261 catcaatggg cagctcgcca atcgcggcgc gccggcggat ttcgaggaat gggtggcgcg
     1321 cggggcgcga ggttggggtt ggaaggatgt gttgccctat tacaaaaagg tcgagcgcga
     1381 catggatttc gacggcgcgc tgcacggtaa agagggccgc attcccgtgc gccggatctt
     1441 ccccgacagt tggaccggac acgccaaggc tgccgccgag gccttcaagg gtgcgggctt
     1501 cgagtacctt cctgatcaga acggggagtg gcgggatggc tattacccga tcgcgatttc
     1561 caatgcttat gagcggcgcg tatcggcggc catcggttac cttgatccag ccacgcgtca
     1621 gcgcaacaac ttgcgcattg ccaccgacac ccaggtcagc gaactcacgt tcgaggggca
     1681 gcgctgcacc ggcgtggtcg ccaacgtcaa gggacagcgc cgcgagttca aggcccgcga
     1741 ggtgatcttg tcctgtggtg ccatccattc gccggcgcac ctcttgcgcg ccgggatcgg
     1801 gcccgccggc cacctcaaag acttgggcct cgaggtaagg catgcctcgc ccggggtcgg
     1861 ccagggcctg atggatcacc cctccatttc cgtgtcagcc tacctcaagc cgcacgggcg
     1921 catgaacgag ttgacgcggc gccatattca cgtgggcctg cggtattctt ccgatatgcc
     1981 gggcatgcac gagggcgaca tgtttgtcgt cgctatcagc aagtcggcct ggcatgcggt
     2041 gggggagcgc atcgcctcgt ttttgatcgc cgtctaccat acggcctcgg agcgcggcga
     2101 ggttaagttg gcatcgcgcg attggcgcga tgagccgcgc gtggagttca acctgctgtc
     2161 tgatcggcgc gatctggagc gcttgatgca ggccttccgc aagatggggc ggctctatgg
     2221 gacagcggcg ctacgcgacg ttacctccga cccgttcccg gccacctact ccgatcgcgt
     2281 gcgcaaggtg ggggtcgtca acaccaagaa taagctaatc acctcgattg ccggcacact
     2341 gctcgatggc ccgcaagcgc tgcgtagcta cctgatccgc accctcatca ccgaaggctt
     2401 cggcttcgac caagtcatgc aggacgacga tgcgctcgaa gctttcgtcc gcaaggccgc
     2461 gatcggggtc tggcatgcct cctgcagcgc gcggatggga gcggaggggg acagcatggc
     2521 ggtgacggac ag
//
LOCUS       DASTMT010000465         4099 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_264788, whole genome shotgun sequence.
ACCESSION   DASTMT010000465 DASTMT010000000
VERSION     DASTMT010000465.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4099
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_264788"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(2..1726)
                     /locus_tag="VFR00_10775"
     CDS             complement(2..1726)
                     /locus_tag="VFR00_10775"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEU0159791.1"
                     /translation="MQAAAPLDAEARPGRRIGGQTVVLGLAVAVLMCLVAYPLAWLLF
                     GAFGLPQSFGLEHLERAFTRPQNFAALLNTLQLALGAGSMSILLGVPLAWATARTDMP
                     LRRLIHALVALSYITPPYLTALAYIILLGPDAGQFNRLLRALFGFASGPINIFSMPGV
                     IFVIGIHVFAFTYFLTHSALKSVDAALEESAQMLGAKPWQTTLRINLPLVAPAITGGA
                     LLAAIESMALFGPQAIIGTPAQIVFLPTRIYATIGGYPPRWGEASALSLLLVVLTVAG
                     LALQRGYLERRSFITVAGRGVRSRTRALGHWRWPVFAFCLLLVFFSAVAPLGVLTLTA
                     FAKSWTEPLSFTNLTLQHFFTALSEDQVAVRGILNSFKLATAAATLAVLIGAAVAYLD
                     VRTSARGRRLLDYLAILPLGLPGTVMAVGILLAFIRMPLPIYGTLSILLIAYVARFLP
                     LATRSANATLRQIDPSLEEAGRATGASWWRAVKLILLPLARPGLLVAFLLVFIPAFGE
                     LSATILLYTGGTETIAIAIYRLNDLGQLEVVSALAVFTIAVVLTLTAVISWLSGAGPA
                     FAETPTRG"
     gene            complement(1768..2823)
                     /locus_tag="VFR00_10780"
     CDS             complement(1768..2823)
                     /locus_tag="VFR00_10780"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEU0159792.1"
                     /translation="MPIARRSLIKAFAAGTTAALLPGRGWAQGAAPAAASAIDLDKAK
                     ADGKVVLYTSLDTQIVDAINAAFKQKYGIDVQYFRGGSSDVTSKVLAEADAGRPQADI
                     VDASDLAALLLMKERGLLKPFAGTAAAAIPKELQDPDATWITDRLTQAVIQYNTNEFG
                     AAPPASWADLGKKAMQGRLVYFSSANGDGAPRIYTLARHLGWDVVKAMAASKPLRVQT
                     PQVITQVLERGERGAGFLQNDNIAWRSKLQGKATNYVFPREGVPSEIGACGLLASSQR
                     PHAAALFYEWWMGAEGQAILVKGGKYSSRLDVAPPEGSTPLAQLKLLTLDYAEYKRDK
                     SKILDQIASIFGGEWGN"
     gene            3019..3924
                     /locus_tag="VFR00_10785"
     CDS             3019..3924
                     /locus_tag="VFR00_10785"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEU0159793.1"
                     /translation="MDYRQLQLFRATAERLNLSHAAEAMNITQPGLSKSMHRLQRELG
                     TKLYHRRGRGIELTESGRALLRHVKTIETQLSDARSEVMGIAGGTLGHARIGAGPSWL
                     SRHLPESIGRVMQQNPNIRFTVDSGFPDRLIGRLRLGELDVVVGALPENRIDPDLRFM
                     RLSSDVIRVVGRKEHPLARRRERSLADYAAQRWILPGRQELVRQRLARAFSLANLAEP
                     VLAVETDSLSLMLATLRMTDCLALTTTQILTQGEAQGIIALDHELLQFTREAGIVSRR
                     HADPSPAVKLLIAQVRKIAAKYGPN"
     gene            complement(3947..>4099)
                     /locus_tag="VFR00_10790"
     CDS             complement(3947..>4099)
                     /locus_tag="VFR00_10790"
                     /inference="COORDINATES: protein motif:HMM:NF021510.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2237 family protein"
                     /protein_id="HEU0159794.1"
                     /translation="PGDRWCLCAERWQEALAAGVAPRVVLAATHEATLEIAELADLKR
                     HALDLA"
BASE COUNT          785 a         1277 c         1368 g          669 t
ORIGIN      
        1 gtcaccctct ggtaggcgtt tcggcaaagg ccgggcccgc gccggacaac cagctgatga
       61 cggcggtgag cgtcaagacg accgcaatgg taaagacggc gagcgcggaa acgacctcga
      121 gttgtcccag atcgttcaag cggtagatgg cgatggcgat cgtttcagtg ccgccggtat
      181 aaagcagaat ggtggcgctc aattcaccga aggccggaat gaagaccaac agaaaggcga
      241 cgagcaatcc cggccgcgcc agcggcagca agatcagctt taccgcgcgc caccaggagg
      301 cccccgtagc gcgcccggct tcctctaaac tggggtcgat ttgacgcaga gttgcattgg
      361 cgctgcgggt tgcaagcgga aggaaacgcg cgacgtaggc gatcaacagg atcgagagcg
      421 tgccgtagat gggcaacggc atgcgaataa aggcgagcag aatgccgacc gccatgacgg
      481 taccgggcag tccgagcggc aggatcgcca gatagtcgag cagccggcgc ccgcgcgcgc
      541 tcgtgcgcac atcgagatag gcgaccgcgg cgccgatgag cacggcgagc gttgctgctg
      601 ccgtcgccag cttgaagctg ttgagaatgc cgcgtaccgc gacctggtcc tcactgagcg
      661 cggtaaaaaa gtgttgtagg gtcagattgg taaaggacag gggctcggtc cagctcttgg
      721 cgaacgccgt cagcgtcagg acccccaacg gggcaacggc ggagaagaac accagcagca
      781 ggcaaaaggc aaataccggc cagcgccaat ggccaagggc tcttgtgcgg ctgcgcacgc
      841 cgcgcccagc cacggtgatg aaggaacgcc gctcgaggta gccacgttgc agcgccagcc
      901 cggccaccgt caatacgacg agcagcagcg acagggcgga ggcttcaccc cagcgcggtg
      961 gatagccgcc gatggtggcg taaatccgcg ttggcaggaa cacgatctgt gcgggcgtac
     1021 cgatgatcgc ttggggaccg aacagcgcca tggactcgat cgccgccaat agcgcgccgc
     1081 ccgtgatcgc cggcgcaacc agcggcaaat tgatgcgcaa tgtcgtctgc cagggcttgg
     1141 ccccaagcat ctgtgcggat tcttccaagg cagcgtcgac cgatttgagc gcgctatgcg
     1201 tgagaaaata ggtaaaggcg aagacatgaa tgccgatcac gaagatgacg cccggcatgc
     1261 tgaagatgtt gatgggtccg gaggcgaaac caaagagcgc cctgagcagc cgattgaact
     1321 gccccgcgtc gggcccaagc aggatgatgt aggcgagcgc cgtcaaatag ggcggcgtga
     1381 tgtaggagag ggccactagc gcgtgtatga ggcggcgcag cggcatatcg gtacgcgcgg
     1441 tagcccaggc cagcggcacg ccaagcaaga tgctcatgct acccgcgccc agcgccaatt
     1501 gcagggtgtt gagcaaagcc gcgaagttct ggggccgcgt gaaggcgcgc tcgagatgtt
     1561 caagaccgaa cgactgcggc aggccgaagg cgccgaagag aagccaggcc aagggatagg
     1621 cgaccaggca catgaggacg gcaacggcga ggcccagcac cacggtttgg ccgccgatcc
     1681 tgcggccggg ccgcgcctcc gcgtcgaggg gtgcggcagc ttgcatttgc cctcatcccg
     1741 caacggacga ttgcgcgcgc agcaagctca attgccccac tcgccgccga aaatgcttgc
     1801 gatctgatcg aggattttgc tcttgtcacg cttatattcg gcgtagtcga gggtgagcag
     1861 tttcaattgg gcgagcggcg tcgagccttc gggcggcgcg acgtcgaggc ggcttgaata
     1921 tttgccaccc ttgaccagga tggcttggcc ctcagccccc atccaccact cgtaaaacag
     1981 tgcggcggcg tgtggccgct gcgagctggc gagcagtccg caggcgccga tttcgctcgg
     2041 cacgccttcc cttggaaaga cgtagttcgt cgctttgccc tgcagcttgg agcgccaggc
     2101 gatgttgtcg ttctggagaa aaccggcgcc gcgttccccg cgctccagga cttgcgtgat
     2161 gacctgcggc gtctgcacgc gcagcggctt gctggcggcc attgccttga ccacgtccca
     2221 gcccaggtgc cgggccagcg tgtagatgcg cggcgcaccg tcaccgttgg cgctggagaa
     2281 atagacgagg cgaccctgca tggccttttt gcccaaatcg gcccagctgg cgggcggcgc
     2341 ggccccgaac tcattcgtgt tgtactggat caccgcctgg gtaagccggt cggtgatcca
     2401 ggtcgcgtcg gggtcttgca gctctttggg gatggcagcg gcagccgtgc ccgcgaacgg
     2461 cttgagcaga ccgcgctcct tcatgagcag cagggccgcc aggtccgagg catcgacgat
     2521 atcggcttgc gggcgacccg catcggcctc tgccagcact ttcgaggtga cgtcgctcga
     2581 gccgccgcga aagtactgca catcaatgcc gtacttttgc ttgaaggcgg cattgatggc
     2641 atcgacgatc tgcgtgtcga ggctggtata gagcacgacc ttgccgtcgg ccttggcctt
     2701 gtcgagatcg atggcgctcg ccgccgcagg ggcggcgccc tgcgcccagc cgcgaccggg
     2761 aagcaaggcg gcggtcgtgc ccgccgcaaa agctttgatc agggatcgtc gggcgatggg
     2821 catcgcagcc tccctcggac ggatgacggc gggtcagccg gcaaacgccg gtgccctgcc
     2881 ggcgggaagg cggcaaaact ggcgccgaag tgcgctccgc tcgccgcccg gtccctctct
     2941 ccggccgaag cgtgcagccc ttgcaggccg cgcacaattg accgagggta gctggaacca
     3001 tgacttgtga gacatagtat ggactaccgc caattgcagc tttttcgcgc aaccgccgag
     3061 cgtctcaatc tcagccacgc cgccgaggcg atgaatatta cgcagccggg cctctccaag
     3121 agtatgcatc gcctgcagag agaactcggt accaaacttt atcatcggcg cgggcgcggt
     3181 atcgagctaa ccgaatctgg gcgggccctg ctgcggcacg tcaagacaat cgagacgcag
     3241 ctttccgacg cccgctccga ggtgatgggt atcgccggcg gcacgctcgg gcacgcccgc
     3301 atcggggcgg gtccctcctg gctcagccgg cacctgccgg aatcgattgg gcgggtgatg
     3361 cagcaaaacc caaacattcg ttttacggtc gacagcggtt tcccggaccg gttgattggg
     3421 cgcctgcgct tgggcgagct ggatgtcgtg gtgggcgcgc tgcccgagaa ccggatcgat
     3481 ccggatctgc gttttatgcg cctgagcagc gatgtgatcc gagtggtggg ccgcaaggag
     3541 catccgttgg cgcgccggcg cgagcgctcg ctcgccgact acgcggcgca gcgttggatc
     3601 ctaccgggtc gccaggagct cgtgcgccag cggctcgcgc gcgcctttag cctcgccaat
     3661 ctggccgaac ccgtactggc ggtcgaaacg gattccttat cgctgatgct tgccaccctg
     3721 cgcatgaccg attgcctcgc cctgacaacg acgcagatcc tgacgcaagg cgaagcgcag
     3781 ggcattatcg cgctcgacca cgagctgttg cagttcacgc gtgaggccgg cattgtcagt
     3841 cgcaggcatg ccgacccatc acccgccgtc aaactgctga ttgcgcaggt gcgcaagatt
     3901 gcggccaagt atggaccgaa ctgattacgc cgtcggcggc tcgaccctat gccaaatcaa
     3961 gggcgtgccg cttcaagtcc gccagttcgg caatttcgag ggttgcctcg tgcgttgcgg
     4021 ccagtacgac acgcggcgcc accccggcag caagggcctc ctgccaacgt tccgcgcaca
     4081 ggcaccagcg gtcgcccgg
//
LOCUS       DASTMT010000466         6121 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_1907772, whole genome shotgun sequence.
ACCESSION   DASTMT010000466 DASTMT010000000
VERSION     DASTMT010000466.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 6121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6121
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1907772"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..438
                     /locus_tag="VFR00_10795"
     CDS             <1..438
                     /locus_tag="VFR00_10795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015934268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase C-terminal domain-containing
                     protein"
                     /protein_id="HEU0159795.1"
                     /translation="TVGAVTATASFRFAKWLDGWFKLSGCNIIIGKGGMTSEIYKRSF
                     VPNNAVYLTTVGYGTGALLGRGIKRVLATYWREELGLAQAIWVLQVENFGPFIADSDL
                     AGNSLFERENAKIAPNIERLYAGSKPAIMRRYGETDDKTDEVI"
     gene            complement(439..1017)
                     /locus_tag="VFR00_10800"
     CDS             complement(439..1017)
                     /locus_tag="VFR00_10800"
                     /inference="COORDINATES: protein motif:HMM:NF013460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HEU0159796.1"
                     /translation="MQDRSAATGSAAVYSKPARRFHWWTAALIAIQAPVGFAMVYRGP
                     TLKIWDGLTNGLYNTHKLLGIVIFFLVLARLGYRLTRGAPPDEPSIEVWQKAAAHATH
                     WALYLLLLLVPIGGYLGISLYPALDVFGIPLPSLTSADQAAAARVFFLHWVGAVLILL
                     LVGAHVSGALFHYFVRKDGVLQRMWRRAGRRA"
     gene            1321..3684
                     /locus_tag="VFR00_10805"
     CDS             1321..3684
                     /locus_tag="VFR00_10805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003615139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0159797.1"
                     /translation="MQSGQAGVKAPPSLRRNETPRDREASSKEATRPAFALLALATLL
                     IAWPWLSGRVTIPWDAKAHFLPQIQFLAQSLAEGQSPFWAPYVFSGHPQIADPQSMIF
                     APPFLALAVINGAPSPWAVDSTLFAMLMLSGAALIVWFRDQGWHWAGGLIGALAFCYG
                     AAMAWRIQHTGQVLSLAYLPLGLLAIDRALARGSLLWGFAFGVVAAAIVLGRDQVALL
                     TIYLIGAFATWRLATAPQPRGALRAAVLPLGAAALTALALAAIPLMLTALFAADSNRP
                     AIDYLEAARGSLHPALLLTLVNPDIFAAAGRMEDYWGPPSFAWRDTGLFVAQNMGVLY
                     LGALPLLLLARAALRGELWQRDIRFFTCAIGLVLLYALGWYTPVFKILYELLPGVQLF
                     RRPADAAFLLGGLGAILAGYAAHRLFLAPAHGFDAATIRGIAGLLAAACAVVLLLAVR
                     LERTGQVVLPLMLAGLAFAAAAIALRLLRPLIAAKPQLAAALLAGVLTLDLAYNNGPS
                     TSSALPTSTYDVLQPRTANATIGALKGSVVENDRRRDRIELVGLGFHWPNASLTHNLE
                     NTLGYNPLRLALYSQAVGAEDHVGLPEQRKFSPLFPSYRSTLADLLGLRFIATSIPIE
                     EIDHHLKAGDLTLITRTGDGYIYENPHAVDRVRFATQAQSADFARLLRDGQWPAVDLA
                     STVLLEGALPSEGPRRRGQVSIVSYQNSEVTLEVDSPDGGWAVLNDVWHPWWYADLDG
                     KPAPLLRANVLFRAVAVPPGHHEIRFTFRPLQGGWAELRQKWARSPG"
     gene            3870..5651
                     /locus_tag="VFR00_10810"
     CDS             3870..5651
                     /locus_tag="VFR00_10810"
                     /inference="COORDINATES: protein motif:HMM:NF017775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HEU0159798.1"
                     /translation="MTAAHFQAPDPPGFRLDPRVIGAFARFAGGYWRDKNAISAWTLT
                     LGLAFALLLSTAATVALNQWNRWFFDALERRDVETVTEAVFVFALIIAAMAAIGVLIV
                     LARETLQVRWRAWIVQHLVERWLGERRFYHLNVSGREPPNPEYRISDDTRWATEPLVD
                     LGIGLLLAVAGAAAFISILWSVGGAIVVTLGSWGTLRIPAYMVWVALAYGMLASGLML
                     WVGAPLVGYVGRKNEAEGYFRFAMMRIRDNAESVALMNGGRYEQAILARFYDTVVARW
                     LAIVWQHAHLTWITNASGPMKPIVPLLFLAPKYLSGELTLGQVTQLAAAFVEVQIAIS
                     WVVDNYNRVAEWYASARRVMDIVEACEATDREALVAKRPVTGTAIRITDFEIADGRNR
                     PLISGTGFAVAAGEAVHIYGESSTGKSTLVRVLSGLWPTARGSVSVPASAEVMIAPQK
                     AYLPLGSLRGALLYPNPALEVSDTRLASALEKVGLGGLAPRLAEVTRWDQVLSNGERQ
                     RLGIARLAIHAPRAVILDDALSALDEGTQAALFAHLRAELPNAIIVSLAQRAAPWSLN
                     DHQLCLERNGESSILRAAGMASMAVAQ"
     gene            5685..>6121
                     /locus_tag="VFR00_10815"
     CDS             5685..>6121
                     /locus_tag="VFR00_10815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0159799.1"
                     /translation="MSTLNFWNTTWSLDEAQCPCDVHFLDYLDERNVKGASIFHFGTG
                     NHHIVGLRTAQNGSDNAVLGITASPHEYQAYIDLLIENPRLGHTYKAYFGDIYQLDAR
                     LLPAFDYVSLFHVGEYRTAENDSYGALSDVDMGLVLADRVKAGG"
BASE COUNT         1002 a         2074 c         1910 g         1135 t
ORIGIN      
        1 accgtcggcg ccgtgacggc gaccgcctcc ttccgcttcg ccaaatggct cgacggttgg
       61 ttcaagctgt cgggctgcaa tatcatcatt ggcaagggcg gcatgacgag cgagatttac
      121 aaacgctcgt tcgttcccaa caacgccgtc tatctcacca ccgtgggcta cggcacgggg
      181 gccttgctcg gtcgcggcat caaacgcgtg ctggccacgt actggcgcga ggaactcggc
      241 ctcgctcagg cgatttgggt gctgcaggtg gagaacttcg gcccctttat cgccgacagc
      301 gatctggcgg gaaattcgct ttttgagcgc gagaacgcca aaattgcccc gaacatcgag
      361 aggctttatg ccggcagcaa gccggccatc atgcggcggt atggcgagac cgacgacaag
      421 accgacgagg tgatctgact aggcccgccg cccggcgcgt cgccacatgc gctgcaacac
      481 accatccttg cgaacgaagt agtggaacaa cgccccgctc acgtgggctc cgaccaacag
      541 caggatgagg acggcaccga cccagtgcag aaagaaaacg cgcgccgcgg ccgcctggtc
      601 ggccgaagtc aggcttggca gggggatgcc gaacacatcg agggccggat acagcgagat
      661 gccgagatag ccgccgatcg gcaccagcag cagcagcaga tagagagccc agtgggttgc
      721 atgcgcagcc gccttttgcc acacctcgat gctcggctcg tccgggggcg cgccccgcgt
      781 caagcggtag ccgaggcggg caagcaccag gaaaaaaatc acaattccca ggagcttgtg
      841 ggtattgtag aggccgttcg tgagcccatc ccagatcttc aaggtcgggc cacgatagac
      901 catcgcgaaa ccgacaggag cctggatggc gatcagcgcg gcggtccacc agtgaaagcg
      961 acgtgccggt ttgctgtaga cggcagcact gcccgtcgcg gcactcctat cctgcatgct
     1021 tggggcctcg tattgcaaag accaagatcg cactgcgata ccacatctac ccggcgccgg
     1081 gcagggcgcc gcaggcctag ggcggcgata ggctgagagg ggtccattgc cggcaaaggg
     1141 cctggccgcc agccaggatc gccggcgcat ccgaggcatc ggggtcagct gcgacggaaa
     1201 gcccggccgg gcaagccgcc aaaagcctgc gctttgccca agtcggacgt tataccccgc
     1261 gcttaggtgc aacagagatg agaccttgcg cgtctctccc tgtgacggtg caacgacgca
     1321 atgcaatcag gtcaggccgg cgtgaaggcg cctccgtcgc tgcgacggaa tgaaacgcca
     1381 cgagatcgcg aggcgagctc caaagaggcg acccgtccgg ccttcgccct cctcgcgctg
     1441 gcaacgctcc tgatcgcctg gccgtggctg tctggccggg ttacgattcc ctgggatgcc
     1501 aaggcgcatt tcctgccgca aatccagttc ctggcgcaga gccttgccga gggccagtcg
     1561 ccgttctggg cgccctacgt gttcagcggg catccgcaga tcgccgaccc tcagtcgatg
     1621 atctttgccc caccgttcct ggcccttgcc gtgatcaacg gtgcaccgag cccgtgggcc
     1681 gtcgacagca cgctgtttgc catgctgatg ctcagcggcg ccgcgctgat cgtgtggttt
     1741 cgcgatcaag gctggcattg ggccgggggg ctgatcggcg cccttgcgtt ttgctacggc
     1801 gcagccatgg cctggcgcat ccagcacacc ggccaggtgc tgagcctcgc ctatctgccg
     1861 ctgggcctgc ttgccatcga ccgggcgctg gcgcgaggct cgttactgtg ggggtttgcc
     1921 tttggcgtcg tggcagccgc gatcgtgctc ggccgggatc aggtggcgct cctcaccatc
     1981 tacctcatcg gcgcgtttgc cacctggcgc ctggccaccg cgccacagcc gcgcggtgcg
     2041 ctgcgcgccg cagtgctgcc gctcggcgcg gccgcactga cggcgctcgc ccttgccgcc
     2101 attcccctca tgctcaccgc actcttcgcc gccgattcca accgcccggc catcgactac
     2161 ctcgaagccg cgcgcggctc cctccatccc gcgttgctgc tcaccctggt taatcccgat
     2221 atctttgccg cggccggccg catggaggac tactggggtc caccgagttt tgcctggcgc
     2281 gataccggcc ttttcgtggc ccagaacatg ggggtgctgt atctcggtgc cttgccgctg
     2341 ttgctgttgg cgcgggcggc gctgcggggc gagctttggc agcgcgatat ccgtttcttc
     2401 acctgcgcca tcggcctggt gctgctctat gcgctcggct ggtacacgcc ggtgttcaaa
     2461 attctctatg agctcctccc cggcgtgcag ctgtttcgcc ggcccgccga tgccgccttc
     2521 ttgctgggag ggctcggcgc catcctcgcc ggctacgccg cgcatcggct atttttggcg
     2581 cccgcgcatg gctttgatgc agcaacgatc cgcggcatcg ccggcctact cgcggcggcc
     2641 tgcgcggtcg tgctcctgct cgccgttcgc ctcgagcgca cggggcaagt ggtgttaccg
     2701 ctcatgctgg ccgggctcgc ctttgccgct gccgccatag cgctgcgctt gctgcggccg
     2761 ctcattgccg ccaagccgca attggcggcc gcgctcctcg caggcgtgtt gaccttggat
     2821 ctggcctaca acaacggtcc cagcacgtcg tcggccctgc cgacaagcac ctacgacgtg
     2881 ctgcagccgc gcacagccaa cgccaccatc ggcgcgctca aaggcagcgt cgttgagaac
     2941 gaccgccgcc gtgaccgcat cgagcttgtc gggcttggct ttcattggcc gaatgccagc
     3001 ctcacccaca atctcgagaa cacgctgggc tacaatcccc tgcgcctcgc cctctacagc
     3061 caggcggtgg gtgcggagga ccacgtcggt ctgcccgagc agcgcaagtt ctcgcccttg
     3121 tttccctcct accgctccac cctcgccgat ctcctgggct tgcgcttcat cgcgaccagc
     3181 attccgatcg aagagatcga tcaccacctc aaagccggcg acctcacgct catcacacgc
     3241 accggggacg gctacatcta tgaaaatccg cacgcggtcg atcgcgtgcg cttcgccacc
     3301 caggcgcaat cagccgattt tgcgcgcctg ctgcgcgacg ggcagtggcc ggccgtcgac
     3361 ctggcttcga cggtcctcct cgagggcgcc ctgcccagcg aaggcccgcg acggcgcggc
     3421 caggtcagca ttgtcagcta tcaaaacagc gaagtgacgc tggaagtcga cagccccgac
     3481 ggcggctggg ccgtcctcaa cgacgtgtgg catccctggt ggtacgccga tctggacggc
     3541 aagcccgcgc cgctgttgcg tgccaacgtt ctgttccgcg cggtcgcggt gccgccgggc
     3601 catcacgaga tccgattcac ctttcgtccc ttgcagggag gctgggccga gctgaggcaa
     3661 aagtgggcac ggtcacctgg atgaggccgg gaccccgccg cgggccggcg ccgcgcgcca
     3721 gccttggaga tttcccctgc agcgatcctg ctcgaagcat gccatgctgg ctcgttcatg
     3781 ccgccttcag cctggccatg agatcggcaa cgggcggtgg catgccttct tgttatgccc
     3841 gcaagaccca aggacgcgtg tgaccctcga tgaccgcagc tcacttccaa gctcctgatc
     3901 ccccgggttt tcggctcgat ccgcgcgtga tcggcgcctt cgcccgtttt gccggcggct
     3961 attggcgcga caaaaatgcg atttccgcct ggaccctgac gctcggcctg gcctttgccc
     4021 tcctgctttc cacggccgcc accgtcgcgc ttaaccagtg gaaccgctgg ttcttcgatg
     4081 ccttggaacg tcgcgacgtg gagacggtga ccgaggccgt cttcgtcttc gcgctcatta
     4141 tcgccgccat ggccgccatt ggcgtcctga tcgtgctggc ccgcgagacc ttacaagtgc
     4201 gttggcgcgc ctggatcgtg cagcacctcg tcgagcgctg gctcggtgaa aggcgcttct
     4261 accatctcaa cgtcagcggc cgcgagccgc ccaatcccga atatcgcata tccgacgaca
     4321 cgcgctgggc caccgagccg ctggtcgacc ttggcatcgg tctattgctc gccgtcgccg
     4381 gcgccgcggc cttcatcagc attctgtgga gcgtgggtgg cgccattgtc gtgacgcttg
     4441 gatcctgggg cacgctgagg atccccgcct acatggtttg ggtggcgctt gcctatggca
     4501 tgctggcctc cggcctgatg ctatgggtcg gcgcaccgct cgtgggctac gtcggccgta
     4561 agaacgaggc ggaaggctat ttccgcttcg ccatgatgcg catccgggac aatgccgaga
     4621 gcgtcgccct catgaacggt ggccgctacg agcaggcgat tcttgcccgc ttctacgaca
     4681 ccgtcgtcgc gcgttggctg gcgatcgtgt ggcagcacgc ccatctcacc tggatcacga
     4741 atgccagcgg tcccatgaaa cccatcgtgc cgctgctgtt cctggcgccg aaatacctgt
     4801 ccggcgaact cacgttgggg caagtcacgc agctggccgc cgccttcgtg gaggtgcaaa
     4861 tcgccatctc ctgggtggtg gacaactaca atcgtgtggc cgaatggtac gcctcggccc
     4921 gccgcgttat ggatattgtc gaggcctgcg aagcgacgga tcgcgaggct ctagtggcga
     4981 agcgaccggt tactggcacg gccatccgca taaccgactt cgaaattgcc gacggccgta
     5041 accggccgct catctccggc accggctttg ccgtcgcagc gggcgaagcg gtacacatct
     5101 acggggaatc cagcaccggc aaatcaaccc tggtgcgcgt gctgtccggg ttgtggccga
     5161 ccgcccgcgg gagtgtcagc gtccccgcta gtgccgaggt gatgattgcc ccgcaaaagg
     5221 cttatctgcc cttgggctcg ttgcgcggcg cgctgctcta tccgaacccg gcgctggagg
     5281 tcagcgacac aaggctggcg tcggcgctcg agaaggtcgg actgggtggc ttggccccgc
     5341 ggctcgccga ggtgacgcgc tgggatcagg tactatccaa tggcgagcgt cagcgcctgg
     5401 ggatcgcgcg gctcgccatt catgctccgc gcgccgtgat cctcgatgat gccctctcgg
     5461 cgctcgacga gggcacccag gccgcactct ttgcgcactt gcgcgccgag ctcccgaacg
     5521 caatcatcgt ctctctcgcc caacgtgcgg ccccatggag cctcaatgat caccagcttt
     5581 gtctggaacg aaacggcgaa agctcgattt tgcgtgcggc gggcatggcg agcatggcag
     5641 tggcacagta ggtcctctcg gttagcacgc ctgggcggtg aacgatgagc acgttaaact
     5701 tctggaatac gacctggagc ctggacgagg cgcagtgccc ctgcgacgtg cacttcctcg
     5761 actacctgga cgagcgcaac gtcaaaggcg cgtcgatatt tcatttcggc acgggcaatc
     5821 atcacattgt cggtttgcgg acggcacaaa acggctccga caacgcggtg ctcggcatca
     5881 cggcttcgcc gcacgagtat caggcttata tcgacctgtt gatcgagaac ccgcgcctcg
     5941 gccacactta caaggcctac tttggcgaca tctaccagct cgatgcccgc ctactccccg
     6001 ccttcgacta tgtcagcctc ttccatgtcg gcgaataccg caccgccgaa aacgacagct
     6061 acggtgcgct gagcgacgtt gacatgggct tggtgcttgc ggacagggtc aaagcaggag
     6121 g
//
LOCUS       DASTMT010000467         2347 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_3547987, whole genome shotgun sequence.
ACCESSION   DASTMT010000467 DASTMT010000000
VERSION     DASTMT010000467.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2347)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2347)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2347
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3547987"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2287)
                     /locus_tag="VFR00_10820"
     CDS             complement(<1..2287)
                     /locus_tag="VFR00_10820"
                     /inference="COORDINATES: protein motif:HMM:NF020083.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HEU0159800.1"
                     /translation="MLERFFDEAGGMQLVIHSPYGSRINRAWGLALRKRFCRKFNFEL
                     QAAATEDSIILSLTTAHSFELPEVVRYLNSASVDNVLIQALLDAPMFTARWRWVAGVA
                     LAIPRFRGGKKLPPQLARMAAEDLIGSVFPDQLACAENLSGEREIPEHPLVDQALADC
                     LHEAMDLDGLKGVLRRLETGAISVVARDLTEPSPLALEVLSARPYAYLDDAPLEERRT
                     QAVMSRRWLDPQTAADIGRLDAAAVTRVQQEAWPEATNADELHDALLWLGFVTTQEAQ
                     QGSGWQQWLTELAGQRRAGLFGAPGAAVWIAAERLLQFTSVWPGAAMQPALAPPAALA
                     AQAWTPEQALVEIVRGRLEGLGPVSQTDLATSLGVGREAMAAALAALQAEGFAMRGRF
                     TPNASEEEWCERRLLARINRYTVKRLRAEIEPVSARDFLRFLLTWQHVDGEARLQGPH
                     TLQTTVEQLQGFEAPAGAWEGEILAARHSDYQSSWLDDGCRSGRVTWMRLGSGVRVGA
                     ARRSGPIKTTPITLLPRENVAMWRAPTDAQTGNLSEHARTVAEFIARHGASFFADIVS
                     GTRLLRCQVEDALAELVAHGMANSDSFGGLRCLLVPSARRRPFAGARRRHRTAAYGMD
                     EAGRWAIITAPYAGEAATTEHVARSLLRRYGVVFWRLLDREATRLPPWRDLLRIYRRL
                     ESRGEIRGGRFVAGVPGEQFALPEAVGLLRATRRQKADHSLVSISAADPLNLIGILMP
                     GPRLPAVTGNRLLFQDGLPVAA"
BASE COUNT          425 a          768 c          776 g          378 t
ORIGIN      
        1 acgccgcaac cggcaggccg tcctgaaaca gaagtcgatt gccagtaacc gcaggcagac
       61 gaggccctgg catgaggatg ccgatgaggt taaggggatc ggcggccgaa attgagacca
      121 ggctgtgatc agccttctgg cgccgcgttg cgcgcaagag gccaacggct tcgggcaagg
      181 cgaactgctc accggggacg ccggcaacga agcgaccacc gcgaatctca cctcgacttt
      241 cgagacgtcg gtagatgcgc agcagatcgc gccacggcgg cagccgggtc gcctcgcgat
      301 ctagcaggcg ccagaacacc acgccgtagc gacgcagcaa gctgcgcgcc acatgctcgg
      361 tggtggcggc ctcgcccgcg tacggcgccg ttatgatggc ccagcgaccg gcctcgtcca
      421 tgccgtaggc ggcggtgcgg tgtcggcgtc ttgcgccggc gaaaggtcgg cgccgcgccg
      481 acggaaccag caagcatcgc agcccaccga aactgtcaga gtttgccatg ccatgcgcaa
      541 ccaattccgc tagggcatcc tcgacttggc agcgcagcaa acgcgtgccc gagacgatgt
      601 cagcaaagaa cgaggcgcca tgccgggcaa tgaactcggc aacggtgcgg gcatgttcgg
      661 aaagattgcc cgtctgtgca tcggtgggtg cgcgccacat ggccacgttt tcgcgcggca
      721 gcagcgtaat cggtgtcgtc ttgatcgggc cggaccgccg cgcggcgccg acgcggacgc
      781 cgcttcccag tcgcatccag gttacgcggc cggatcggca accgtcgtcg agccagctcg
      841 attggtagtc gctgtgccgc gcggcaagaa tctcgccctc ccaggcgccg gccggcgcct
      901 caaaaccttg cagttgctcg acggtcgttt gcagcgtgtg tggaccttgc aggcgcgcct
      961 cgccgtcgac gtgttgccag gtcaataaga agcggaggaa gtcgcgcgcc gacaccggct
     1021 cgatctcggc gcggagccgt ttcaccgtgt agcgattgat gcgcgccagc agcctgcgtt
     1081 cgcaccactc ctcctcgctg gcgttgggcg tgaagcgacc tcgcatggca aacccctccg
     1141 cctgcaaggc cgctagagcc gccgccatgg cctcgcgccc gacgccaagc gaggtcgcca
     1201 gatcggtctg gctaaccggg cccaagccct ccaaacgccc gcgcacgatt tcgaccaagg
     1261 cctgctccgg tgtccaggct tgcgccgcca atgccgcggg tggcgcaagg gctggctgca
     1321 tcgccgcgcc gggccaaaca gaggtaaact gcaacagcct ctccgccgcg atccaaaccg
     1381 ctgcgcccgg ggcgccgaac aggccggctc gccgttgccc ggccagctcc gtcaaccatt
     1441 gctgccaccc gctgccctgc tgggcttcct gggtcgtaac gaagccgagc caaagcaatg
     1501 cgtcgtgcag ctcatcggca ttggtggcct ccggccaggc ttcctgttgc acgcgggtga
     1561 cggccgccgc atcgagacga ccgatatccg cggccgtctg cggatctaac cagcgccggc
     1621 tcatgacggc ttgggtacgt cgttcttcga gcggggcatc gtcaagatag gcgtagggcc
     1681 tggctgatag cacttcgagc gccaggggtg atggttcggt caggtcgcgg gcaactacgc
     1741 ttatggcacc tgtttcgagc cggcggagca cgcccttcag cccgtcgagg tccatcgcct
     1801 cgtggagaca gtcggcaagg gcctgatcga ccaaaggatg ttcgggtatc tcgcgttcgc
     1861 cgctgaggtt ttcggcgcag gcgagctgat cgggaaagac gctgccaatc aaatcctcag
     1921 ccgccatgcg cgccagttgc ggcggcagct ttttgccgcc gcggaagcgg ggtattgcca
     1981 aggcaacgcc ggcaacccag cgccagcgcg cggtgaacat cggcgcatcg agcagggctt
     2041 ggatcaaaac gttgtcgacg ctggcggagt tgagatagcg gacgacctcg gggagctcga
     2101 agctgtgcgc tgtcgtcagc gaaaggatga tgctgtcctc ggtggctgcc gcctgcagct
     2161 caaaattgaa tttgcgacag aagcgtttac gcagtgccag gccccaagcc cgattgatac
     2221 ggctgccgta gggtgaatgg atgaccaatt gcatgccgcc ggcttcgtca aagaagcgct
     2281 ccagcacgat ggtatcatgt gtgggcagac agccgagcgc ggcccgcgac gcggccagat
     2341 actcgac
//
LOCUS       DASTMT010000468         3522 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_2238423, whole genome shotgun sequence.
ACCESSION   DASTMT010000468 DASTMT010000000
VERSION     DASTMT010000468.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3522)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3522)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3522
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2238423"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            18..386
                     /locus_tag="VFR00_10825"
     CDS             18..386
                     /locus_tag="VFR00_10825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018426263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6481 family protein"
                     /protein_id="HEU0159801.1"
                     /translation="MREYKDQNRLERLERAAKAKQQILDKFRARPAADDPNVLARAAE
                     RKAVAEAREAREKERAERKRIEAEERAQREAVEKAEREARERREAVEKVIRDAADAAE
                     RKAERDRRYAARKARQAGRR"
     gene            complement(681..1004)
                     /locus_tag="VFR00_10830"
     CDS             complement(681..1004)
                     /locus_tag="VFR00_10830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0159802.1"
                     /translation="MARAWIFAGLLLAATAATAHDWYPIECCSGMDCAPVEKVEIVSV
                     QSAGIMGSSSLPGTMLITTKHGSVIVPANFPRRESKDNRMHACIRASAGGNMRLICLF
                     MPPST"
     gene            complement(1430..2524)
                     /locus_tag="VFR00_10835"
     CDS             complement(1430..2524)
                     /locus_tag="VFR00_10835"
                     /inference="COORDINATES: protein motif:HMM:NF016064.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HEU0159803.1"
                     /translation="MASIREALLLNAPLSLALGGFLIGGAFGAVVFRTNYCAMGSLSD
                     IHNFADYRRFRAWLLAAATAILGAQLLELAGIVALDQSMYLTPTLNWAANALGGVVFG
                     IGMVFAGGCPSRNLARAGAGDLRALITLVVLGLTAYMAIGGLLGPLRARFEAATAIAL
                     PLPSQGLPHLLGAIVPAPVSVMKLIAAAGLVAGALFYCAYDAKFRASRVHVFSGLAVG
                     ACVIAGWALTGLAFDEFAARPLQPVSLTYVRPAGDALEWLQRFTAGPVPSFGVASVFG
                     ALLGAFAAAKAMGRFRLIGFSDTGDMIRSLGGAALMGVGGVLAFGCTVGQAITGVSTL
                     AFGSLLTFAAIVLGGFWGLRLLERTLLDAN"
     gene            2763..>3522
                     /locus_tag="VFR00_10840"
     CDS             2763..>3522
                     /locus_tag="VFR00_10840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013948493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEU0159804.1"
                     /translation="MTAAADPAFVHVKVHSAYSLLEGAITIAKLAKLAVAQGFPALGL
                     TDSGNLFGALEFSDKLAAAGVQPIVGMTLKVDFGEPDRTGPGRPLAPQARLSSAGVVA
                     LIACNERGYQNLMQLSSRAFLDPAENEPPHVKLSQLQGDRAQGLIALTGGPDGPINRA
                     LSEEQRELAGERLAALAAIYGDRLYVELQRHGLAQDLKTEPELIALAYARSLPIVASN
                     EVYFASPDDYEAHDALLCIAEGSYVTEDNRRRLSR"
BASE COUNT          689 a         1144 c         1154 g          535 t
ORIGIN      
        1 atgcgagaag gactgcaatg agagaataca aggaccagaa tcgtctcgag cggctcgagc
       61 gggccgccaa agccaaacaa caaatcttag acaagttccg ggcgcgtccg gcggccgatg
      121 atccaaacgt tttggcccga gcggccgagc gcaaggccgt cgccgaagcg cgcgaggctc
      181 gggaaaagga gcgcgccgag cgcaagcgca tagaggccga agagcgggcg cagcgggagg
      241 ccgtcgaaaa ggcagagcgg gaggcccgcg agcgccgcga ggccgtcgaa aaggtcattc
      301 gcgatgcggc ggatgctgcc gagcgcaaag ccgaacgcga tcgtcgctat gccgcgcgca
      361 aagcgcgcca ggcaggccgg cgctaggctg aggtgaaccc cacgtcaggg ggcaagcttt
      421 tgagctgccg cccttcgcgt gtgcggcctc gttgcgactt tgattggccc tgagggttac
      481 gcggcgcggc tggccaatgt tcggccggca ggcacggctt ttcgcgtatt gcgctcaatt
      541 cgacagcacc agcgcggttg ccgctgccgt gtcggcaagc tcctcgacgc tatacaagcg
      601 gtgcgcctaa tcgcgtctgc tgggcgcggc tgtgggaggc gggcctcccg ttgacgtgct
      661 gcgggcaggc gcgcgccggc tcaggtcgat ggtggcataa acaggcagat gaggcgcatg
      721 ttgccacccg cggaagcgcg gatgcaggca tgcatgcgat tgtctttcga ttcgcggcgc
      781 gggaaattcg cgggcacgat cacgctgccg tgtttggtgg taatcagcat ggtgccggga
      841 agcgatgagc tgcccatgat gccggcgctt tgcacggata caatctcaac cttctcgaca
      901 ggcgcgcaat ccatgcccga gcagcattcg atcgggtacc aatcgtgggc cgtcgccgcc
      961 gttgctgcca acagcaaacc tgcgaatatc catgctcttg ccataatatg cctcgctggt
     1021 ttcgcgcctc tttcctcctc gagtgtttcc cgccctagtg ttatcgaggc gcggtccgcc
     1081 attgcggtcc cacgctccgt tgcgacgggc tcattcgaca cgagccagtt cgcaacggcc
     1141 ccgtcccaca ggcacggtct gcgaaagggg cccgtgcgca tggtcgtgca aatggattta
     1201 ttgcaagtgc cgtgccccgc gccgccgtct ctgtttgaag acggtttaat gacagggatt
     1261 gtgaagcgat tcggccaggg cgtccttgca cgcctgtgcg tttccggacg ccgacggggg
     1321 atgttgatac aaacgccaag ttcatgatgt cattccgcct cagcccgttc ggccggggct
     1381 tgctcgtaaa ttgggcaagg attgcccacc ccgacgcgga gttaagcaat caattcgcat
     1441 ccagcagggt tcgttccagc aggcgcaatc cccaaaaacc cccaagcacg atggcggcaa
     1501 acgtcaggag ggagccaaag gccaatgttg acacgcccgt gatggcctgt ccgaccgtgc
     1561 agccgaaggc cagcacgccg cccacaccca tcagggccgc gccgccgagg ctgcggatca
     1621 tatcgccggt atcggaaaag ccgatcagtc ggaaacggcc catcgcctta gccgcggcga
     1681 aggcgccgag gagggcgccg aacacgctgg caaccccgaa actcgggacg gggccagcgg
     1741 tgaaacgctg cagccattcc aaggcatcgc ccgccggccg cacataggtg agcgataccg
     1801 gctgcagcgg ccgggcggcg aattcatcga aagcgagccc cgtcagcgcc caaccggcaa
     1861 tgacgcaggc gcccaccgcc aacccggaga acacgtgcac gcgtgaggcg cgaaacttgg
     1921 cgtcataggc gcaatagaag agcgcgccgg ccaccagtcc cgccgcggcg atgagcttca
     1981 tgacgctcac cggcgccggc acgatggcgc ccaacaaatg aggcaggccc tggctgggca
     2041 gcggcagcgc aatcgccgtc gccgcctcga agcgggcgcg cagtggcccg agcaagccac
     2101 cgatcgccat ataggcggtt agccccagca ccacgagggt gatgagcgcg cgcagatcac
     2161 cagcaccggc acgcgccaga ttgcggctcg ggcagccgcc ggcgaacacc atgccaatgc
     2221 cgaacacgac gccgcccagg gcattggccg cccagttcag cgtaggcgtg agatacatcg
     2281 attggtccaa ggccacgata cccgctagct cgagcagctg cgcacccaag attgccgtgg
     2341 cggcggccag cagccaggcg cgaaaacggc ggtagtcggc gaagttatga atgtcggaga
     2401 gcgagcccat ggcgcagtag ttggtcctga acacgacggc accgaaggct ccaccgatca
     2461 ggaagccacc gagcgccaat gagaggggcg cgttcaagag cagcgcctcg cgaatagatg
     2521 ccatgagcgt ccttggttgt taggctcgac gggccacccc gcagcggcta gatggtggcg
     2581 gcagcggccc agagcgaggg ccggcagccc ggcggcagca acggtccatg ccgtggaccc
     2641 aatccgcgca atccacaagg ccgcgctcgc ggccgcccgc gcgcttgcgg caattgcacg
     2701 ttggcctgaa agattgtgca ttctgagcca tgacggcgat gattcgcatg aacgaggcga
     2761 gaatgacagc agcagcagac ccggccttcg tgcacgtcaa agtgcactca gcctattcgc
     2821 tgctggaagg cgccatcacc attgccaagc tcgccaagct ggccgtggct cagggctttc
     2881 ctgccctcgg tctgaccgac agcggcaacc tcttcggcgc cctggaattt tccgacaaat
     2941 tggcggccgc cggggtgcag ccaatcgtcg gcatgacgct caaggtcgac ttcggggagc
     3001 cggaccgcac cgggcctggc cgcccgctcg cgccgcaggc gcgccttagc tcggccgggg
     3061 tcgtcgcgct gatcgcctgc aacgagcgcg gctatcaaaa cctgatgcag ctctcttcgc
     3121 gggcgttcct tgatcccgcc gaaaacgagc cgccgcacgt caagctgagc cagctgcagg
     3181 gggatcgcgc ccaaggtctg attgcactga ccgggggacc ggacggacct atcaaccgcg
     3241 cgcttagcga agagcaaagg gaactcgcgg gcgagcgcct ggcggcgctc gcggcgatct
     3301 acggcgaccg gctctacgtc gagctccagc gccacggcct ggcgcaggac ctcaagaccg
     3361 agcccgaact catcgcgctt gcctatgcgc gctcgctgcc gatcgtcgcc agcaacgaag
     3421 tctactttgc gagccccgat gactatgagg cgcacgatgc gcttttatgc atcgccgagg
     3481 gcagctatgt gaccgaagac aatcgccggc ggctctcgcg cg
//
LOCUS       DASTMT010000469         2222 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_3221827, whole genome shotgun sequence.
ACCESSION   DASTMT010000469 DASTMT010000000
VERSION     DASTMT010000469.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2222)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2222)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2222
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3221827"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..810)
                     /locus_tag="VFR00_10845"
     CDS             complement(<1..810)
                     /locus_tag="VFR00_10845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015665232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005488 - binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DctP family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HEU0159805.1"
                     /translation="MNRREVLKGSSALVAAAATGIPAATAQAPIVIKFSHVVATDAPK
                     GKASDKFKELAEKYTGGKVKVEVYANSSLYKDKEELEALQLGAVQMLAPSTSKFGPLG
                     FSEFEALDLPFIFHDEAAFQAFAKSELAKQMFAKLETKAVKGLAYWDNGFHVMGANRQ
                     IRNVKDFQGLKLRIQGSKVLDATTKAWGAIPQVMAFSEVYHALQTGVVDGLENVPSNF
                     WTQKFFEVVKYIAVSYHGHLSYAVVANKAFWDGLPADVRPGLDKAVAEASAY"
     gene            complement(966..1874)
                     /locus_tag="VFR00_10850"
     CDS             complement(966..1874)
                     /locus_tag="VFR00_10850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HEU0159806.1"
                     /translation="MAALDYHVLDVFTTERFGGNPLAVVLDADGLDGKRMQTIAREFN
                     LSETVFVLQPSHAAHTARVRIFTPAAEMPFAGHPTVGTACLLAQLRLPPAPQESEALI
                     VLEEEIGPVRVGVRLRRGSAPFAEFDAPRLPEDLGSPPAVDRLAAAVGLIPAEIGFEN
                     HRPTRYSAGNAFLFLPVASLEAIGKAHVSSGHWEAGMGTGTGAFLYCRQTVHTTSAFH
                     ARMFAPHHGIAEDPATGSAAAAFAGVVQRFDELPDGTHKRTIEQGFEMGRPSLIDLSV
                     TVAAGRLSTVRIGGHAVRVAAGSIEV"
     gene            complement(1883..>2222)
                     /locus_tag="VFR00_10855"
     CDS             complement(1883..>2222)
                     /locus_tag="VFR00_10855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0159807.1"
                     /translation="PAHKAAVLRAITAAAAKERCARPCGPWVAAKLATAPSPVKKRTL
                     ARRLRRRECALAFDDSVALRWRSRGSGRSGQELIPLQHHETKLRSCFAGVVVLEVTRK
                     YANVPAPQQA"
BASE COUNT          396 a          744 c          682 g          400 t
ORIGIN      
        1 ataggcgctg gcctccgcca cggccttgtc gaggcccggc cgcacgtcgg caggcaaacc
       61 gtcccaaaac gccttgttgg ccaccaccgc gtaggaaaga tgcccgtggt aggagacggc
      121 gatgtatttc accacctcga agaacttctg ggtccagaaa ttcgagggca cgttctcgag
      181 cccatcgacc acgcccgtct gcagcgcatg gtacacctcc gagaaggcca tcacctgtgg
      241 aattgccccc caggccttgg tggtggcgtc gagcaccttt gaaccctgga tgcgcagctt
      301 gaggccttgg aagtccttga cgttgcggat ctgccgattg gcacccatga cgtggaagcc
      361 attgtcccaa taggcgaggc ccttcacggc cttggtttcg agcttggcga acatctgctt
      421 ggccagctcg ctcttggcaa aggcttggaa agccgcttcg tcgtggaaaa tgaagggcag
      481 gtcgagcgcc tcgaactcgc tgaagccgag tggtccaaac ttcgaggtgg agggcgccag
      541 catctgcacc gcccccaact gcagcgcctc caattcttcc ttatctttgt acagggacga
      601 gttggcgtag acctcgacct tgaccttgcc gcccgtgtac ttttccgcca gctccttgaa
      661 cttgtccgag gccttgccct tcggcgcatc ggtggccacc acgtggctga acttgatgac
      721 gatgggagcc tgagccgtgg ccgcagggat cccggtcgcc gccgccgcca ccagcgcgct
      781 cgaacccttt agcacctcac gtctgttcat cttgcgacct cttttcccgt ttcacttcgc
      841 ctgtccactc gggaggcacg ttgtcgccgg ttatagcgag ctcgcccgag cctgaccacg
      901 ataccttcgt tgcgtgagcc taggcccagc cttgccgcgc cgggggcgcc ggacctaagg
      961 attgcctaga cctcgatgct gccggcggcc acgcgcaccg catggccgcc gatcctgacc
     1021 gtactgagcc tgccggccgc caccgtgacc gacaggtcga tcaggcttgg ccgtcccatc
     1081 tcgaaaccct gctcgatggt gcgcttgtgg gtgccatccg gcagctcgtc aaagcgctgc
     1141 acgacgccgg caaacgccgc cgccgccgag cccgtggcgg gatcctcggc aatgccgtgg
     1201 tggggcgcaa acatgcgggc gtgaaaggcc gaggtcgtgt gcacggtttg gcgacagtat
     1261 agaaaggccc ccgtgcccgt ccccatgccc gcctcccagt ggccgctcga gacgtgcgcc
     1321 ttgccgatgg cctcgaggga agccaccggc aagaacagaa aggcattgcc cgccgaatag
     1381 cgggtcgggc ggtggttttc gaaaccgatc tcggccggga tcagcccgac cgccgcggcc
     1441 aagcggtcga cggccggcgg actgccaagg tcttcaggca accgcggcgc gtcgaactcg
     1501 gcgaacggcg cactgcctcg acgcaggcgc accccgacac gaaccggtcc aatctcctcc
     1561 tcgagcacga tcagggcttc gctttcctgc ggcgccggcg gcaggcgcag ctgcgctagc
     1621 aggcacgccg taccgaccgt ggggtgcccg gcaaagggca tttccgccgc cggcgtaaaa
     1681 atgcgcacgc gcgcggtgtg ggcagcatgg ctcggctgca acacaaaaac cgtctcggac
     1741 aggttgaact cgcgggcaat cgtctgcatg cgcttgccgt cgagaccatc ggcatcgagc
     1801 acgacggcca gaggattgcc gccgaagcgt tcggtcgtga agacatcgag aacatgatag
     1861 tcaagggcgg ccatgctcac gctcatgctt gctgcggagc aggcacattg gcgtatttcc
     1921 tggtcacttc caagaccaca acgccggcaa agcacgatct caacttcgtt tcgtgatgtt
     1981 gcagtgggat caattcttgc cccgatctgc cgctgccacg cgaacgccat ctcagcgcca
     2041 cgctgtcgtc aaatgccagc gcacactcgc gcctgcgcaa acgacgcgcc agtgtgcgtt
     2101 ttttcacggg ggaaggagca gttgcgagtt ttgctgcaac ccagggaccg caagggcgcg
     2161 cgcagcgctc cttcgcagcg gcggcagtga tcgcccgcaa cactgccgcc ttatgtgctg
     2221 gc
//
LOCUS       DASTMT010000470         2775 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_3222086, whole genome shotgun sequence.
ACCESSION   DASTMT010000470 DASTMT010000000
VERSION     DASTMT010000470.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2775)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2775
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3222086"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..830
                     /locus_tag="VFR00_10860"
     CDS             <1..830
                     /locus_tag="VFR00_10860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU0159808.1"
                     /translation="EGELRTLPTVYRPDLFKGKVVVVTGAGSGFGFAIATLFARLGAG
                     LAILGRNEERLLKAKSFFESLGAVVYAEALNIRDPERCAAFITNVWREQARLDVLINN
                     AGGQFPQPALDFKPKGWTAVIDNNLNGTWYMMQAAAKSWVERTSAGAIVNIVADFWRG
                     MPGIAHTAAARAGVAYLSKSVAVEWAPLGIRVNCIAPGVLETSGFAHYPPAGLKTYTE
                     ANPLRRAGDVWDVAEAAVYLAAPAAKFITGEVLTVDGGQQCWGDPWPSGRPDHFKVG"
     gene            886..1875
                     /locus_tag="VFR00_10865"
     CDS             886..1875
                     /locus_tag="VFR00_10865"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HEU0159809.1"
                     /translation="MPGIERRAVLRGLIAAACAGTVHVSNQAHSQGREQPLRLVFPFA
                     AGGSGDALARLLAEHIRIGLGVPVIVENKTGAAGRIGVQAVKGAPADGSTILLTPIAP
                     VAVYQHVYDPLGYDPVADFEPVSQVASFEFAVAVGPQVPAKTLKELVDWVRTNPGAAN
                     YGTPAAGTLPHFFAVSFARASGLDLRHVGYRGSAAAITDLVGGQISMVFTTTSDLLEM
                     HKAGRIRILATSDQQRSPLLPDIPTFKEEGYAIQGTAWYAMYAPAKTPPEVVTRLNEV
                     IVGALRRPELKERLLALGLYATGTSPAELAKIQKADAELWAPAIKASGFTPQQ"
     gene            complement(1976..2239)
                     /locus_tag="VFR00_10870"
     CDS             complement(1976..2239)
                     /locus_tag="VFR00_10870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0159810.1"
                     /translation="MPANDGTPDGWYGLIKSEAGRVALATRRLAYDFQATARAMRAHG
                     YADDYAQAIASGLWPSLDILPAAERSAAGTRIADCSALFELCP"
     gene            complement(2245..>2775)
                     /locus_tag="VFR00_10875"
     CDS             complement(2245..>2775)
                     /locus_tag="VFR00_10875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEU0159811.1"
                     /translation="FGGPYSNMRALLALRACAHALAIPADRCICTGDAVAYCREPEET
                     IGLLRDWGCQVIAGTCEEPLAAGAADCGCGFASGSECDRLAKGWYAFADQRVSRANRA
                     WMATLPKTLTFQEGGVSFRVVHGGVDVVNRFVFASDHDLIAAELARAEADIVVAGHCG
                     LPFIHGWARRSGSTRA"
BASE COUNT          507 a          949 c          842 g          477 t
ORIGIN      
        1 cggaggggga gcttcgtacc ctgccgaccg tctaccggcc cgacctgttc aagggcaagg
       61 tcgtcgtcgt caccggtgcc ggcagcggct tcggttttgc catcgccacc ctgttcgccc
      121 gcctcggtgc cgggctcgcc atccttggcc gcaacgagga gcgcttgctc aaagccaaga
      181 gctttttcga aagcctcggc gccgtggtct atgccgaggc attgaacatc cgcgatcccg
      241 aacgctgcgc cgcctttatc accaacgttt ggcgcgagca ggcgcgcctc gatgtgctca
      301 tcaacaatgc cggcggccaa ttcccgcagc cggccctcga cttcaaaccc aagggctgga
      361 cggcggtcat agacaacaac ctcaatggca cctggtacat gatgcaagcg gccgccaaat
      421 catgggtaga gcgcacctcg gcgggcgcca tcgtgaacat cgtcgccgac ttctggcgcg
      481 gcatgccggg cattgcccat accgccgccg cgcgcgccgg cgttgcctac ctctcaaaga
      541 gcgttgccgt ggagtgggca ccgctcggca ttcgcgtcaa ctgcatcgcg ccgggcgtgc
      601 tggaaacctc aggcttcgcc cattatccgc ccgcgggtct caagacctat actgaggcca
      661 atccgctgcg cagggcaggc gacgtctggg acgtggccga agcggcggtt tatttggcgg
      721 cccccgcggc taaattcatt accggcgaag tccttacggt cgatggcggc cagcagtgct
      781 ggggtgatcc ctggcccagc ggccggcccg atcatttcaa ggtgggatga gccggaggtg
      841 ggctggcgcc cgctgggacg tgtgatagcc gagggaggaa agcggatgcc agggatcgag
      901 cgaagggcgg tgttgcgagg tcttatcgct gccgcctgtg ccggaacggt gcacgtctcc
      961 aaccaagctc actcacaagg ccgcgagcag cccctgcgcc tcgtctttcc atttgctgcc
     1021 ggcggctcgg gcgacgcgct ggcgcgtctg ctggccgaac acatacgcat tggtctcggt
     1081 gttcccgtca tcgtcgaaaa caaaaccggc gccgccggtc gcattggcgt tcaagcggtg
     1141 aagggcgccc ccgccgatgg cagcaccatc ctgctcacgc cgatcgcgcc cgtcgccgtc
     1201 taccaacatg tctatgaccc actgggctac gatccggtcg ccgatttcga gcctgtgtca
     1261 caggtggcca gctttgaatt cgccgtcgcc gtcgggccgc aggttccagc caagacgctg
     1321 aaggagttgg tcgactgggt gcgaaccaat cccggcgccg ccaactacgg cacccccgcc
     1381 gccggcacgt tgccgcactt ttttgccgtc tcctttgcgc gggccagcgg cctcgatctg
     1441 cgccatgtcg gctatcgggg ctccgccgct gccatcaccg acctcgtcgg tggccagatt
     1501 tcgatggtgt tcacgaccac ttccgacctg ctcgaaatgc acaaggccgg gcgcatcagg
     1561 atccttgcca cgtcggacca gcaacgttcg cccctgttgc ccgatattcc caccttcaag
     1621 gaggagggct atgccataca aggcacggct tggtacgcca tgtacgcgcc cgccaagacc
     1681 ccaccggaag tcgtgacccg gctcaacgag gtgatcgttg gcgccctgcg ccggccggag
     1741 ctcaaggagc gattgctggc cctcggtctc tacgccaccg gcacgtcacc tgccgagctg
     1801 gcaaagatcc aaaaggcgga cgctgagctg tgggcaccgg ccatcaaagc ctcgggcttc
     1861 accccccagc aatagtgacc cacaacctgc aaccgaagcc aaggccccgc aatcctcgcc
     1921 tcgagcgaac aaagcaagcg cgcccttgct gcggcagctg agcaagcgag caaattcaag
     1981 ggcacagctc aaacagggcc gaacagtccg caatccttgt gcccgccgcc gatctctccg
     2041 cggcgggcaa gatatcgagg ctcggccaaa ggccgctcgc gatcgcctgc gcgtagtcgt
     2101 ctgcgtagcc atgcgcacgc atcgctcttg ccgtcgcctg aaaatcgtag gcgaggcgtc
     2161 gggttgcaag tgccacgcgt cccgcctccg acttgatcaa cccataccaa ccatcgggcg
     2221 tgccatcgtt ggcgggcatg ccgatcacgc ccgggttgaa ccagacctgc gcgcccaccc
     2281 atgaatgaag ggcagtccgc agtggccggc cacaacaatg tcggcctctg cccgtgcaag
     2341 ctctgccgcg atgaggtcat gatcggaggc gaacacaaag cggttgacga cgtcgacgcc
     2401 gccgtggacg acacgaaagc tcactccccc ttcctgaaag gttagcgtct tgggcaacgt
     2461 cgccatccat gcccggttgg cgcgcgaaac gcgctgatcg gcgaatgcat accatccctt
     2521 ggcgagacga tcacactccg atccggatgc aaaaccgcaa ccgcaatcgg cagccccggc
     2581 tgccagtggt tcttcacaag tgccggcaat cacctggcag ccccagtcgc gcagcaggcc
     2641 aatggtttcc tcgggttccc tgcaataggc caccgcgtca cctgtgcaga tgcatctatc
     2701 ggcggggatt gcaagggcgt gtgcgcaggc tctgagcgca agcaaggcgc gcatgttcga
     2761 gtagggtccg ccaaa
//
LOCUS       DASTMT010000471         3176 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_2891868, whole genome shotgun sequence.
ACCESSION   DASTMT010000471 DASTMT010000000
VERSION     DASTMT010000471.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3176)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3176
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2891868"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2890)
                     /locus_tag="VFR00_10880"
     CDS             complement(<1..2890)
                     /locus_tag="VFR00_10880"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEU0159812.1"
                     /translation="MEAKSASQAAAQASRKVATVLFADIKGSTKAISAIDAEAAMLRI
                     RPMINAMAGAVREQGGTVIEVRGDGILSGFGALDSLEEHPLAACRAALAIRQAAKGGA
                     SPGLPVRIGIHTGEVIVTPGEAGNSMIGATVHLASRLETAAEPNTILISEDVYRAVAT
                     TAEVQPRGRFTFKGFDQPVETWELLDVEHRSRWNTRALMGLTPYCGRAPERALLKSFI
                     EQTKQGLGGVLSVSGDPGTGKSRFIHDALTSGLVDDCTIWFAESELPARHSPYAIARS
                     IARSWLGLTDHNTAQDVERRLSAVLQQFADTLAEHAPALKALLNLSVAGTDWANYDLI
                     DRKRRLAACFLEISRLQARHRPLLIIVDDAQWCDPETLQVLAEFAHHIQAMSAGLILL
                     ERSGSDTPLVDAFATASTIRLRELSAQETQDFLGVVLGSDPSLEPVKAKLTDMAGGLP
                     FFLEEAVRYLSRNGILVGDAGSYAVVAQAGAINIPDTVEALVSSRISSLTPDLRSVLS
                     AASIVGRTGPLALIARICDSSVADVQPKLENLERQHIIAISELKSETIFEFRHEFFRQ
                     VAYAMTLSSDRRVLHAKTLRAAEVLFDNRISDWITFLAYHAALASLHADAARYCRIAA
                     EHAVLASSYSAASEFCEQAVKHLDQLPQTVENIQASIDVRLLLRVAAGGTSDFQSWLH
                     HLTKSIELAEQIGDMSRRLLALIHRTSAINFSSSAVDAVRAGEAAVALAQALRIPSSE
                     ALARYALGQAHYVNGSYRDAIESLTPATQWLAQGHEMEQLGTTGTTLVLCLMMQANSH
                     ASMGHFQAADGELARLDDLASRTGRSYDQVALSYCRGMVWGGQGQLDPTINAFEYAYE
                     LCRRHGINLFVPVIAAGLSAVLVASRKVARASEVALVGLEVAERLGHNVARTAATAAL
                     AGVRIAEGNPREAIRLASEARSSASAYAHRGVEVAATRVLAQAFLASNP"
BASE COUNT          560 a          991 c         1088 g          537 t
ORIGIN      
        1 cggggttgct cgccagaaag gcctgggcca gcacgcgcgt ggctgcgacc tcgacgccgc
       61 gatgggcata ggcgctggcg gatgaccgcg cctcgcttgc cagccgtatg gcctcgcgcg
      121 gattgccttc ggcgatgcgc acgccagcca gcgccgccgt ggcggcggtg cgcgcgacgt
      181 tgtggccgag ccgctcggcc acctccaagc cgaccagggc cacttcgctg gcgcgcgcga
      241 ccttgcgtga cgccaccagg acggcgctga ggccggcggc gatgacgggc acgaacaggt
      301 tgatgccgtg ccggcggcag agctcgtagg cgtactcaaa ggcattgatg gtgggatcga
      361 gctggccttg gccgccccac accatgccac gacagtagga cagcgccacc tggtcgtagg
      421 atcgccccgt gcggctcgcc agatcgtcga ggcgggcgag ttcgccgtcg gcggcctgga
      481 agtgccccat cgaggcatgc gaattggctt gcatcatcag acacaggacg agcgtggtcc
      541 ccgttgtgcc gagctgctcc atctcatggc cctgggccaa ccattgcgtg gccggtgtca
      601 ggctttcgat ggcatcgcgg tagctgccat tgacatagtg ggcctgtccc agcgcatagc
      661 gggccagcgc ctcgctcgag gggatgcgca aagcctgcgc cagggcgacc gccgcctccc
      721 ccgcgcgcac cgcatcgacc gccgagctcg agaaatttat cgccgaggta cggtggatga
      781 gggcgagcag ccggcgcgac atgtcgccga tttgctcggc gagctcgatg gacttcgtca
      841 gatgatgcag ccagctctga aaatcggagg tgccgccggc ggccacacgc aacagcaggc
      901 gcacgtcgat cgaggcctgg atgttttcca ccgtctgcgg caactgatcc aagtgcttga
      961 cggcctgctc gcaaaactcg gaagcggcgc tgtaggagga ggcgagcacg gcgtgttcgg
     1021 cggcgatgcg acagtagcgt gccgcatcgg cgtgcaggct cgccagcgcc gcgtgatagg
     1081 ccaggaaggt gatccaatcg gaaatgcggt tgtcgaacag cacttcggcg gcgcgcaagg
     1141 tcttggcgtg cagaacgcgc cggtcgctgc tcaaggtcat cgcgtaggcg acctggcgaa
     1201 agaactcgtg acgaaattcg aagatggtct cggacttcag ctccgagatg gcgatgatgt
     1261 gttggcgctc gagattttcc agcttcggct gcacgtcggc aaccgaactg tcacaaatgc
     1321 gcgcgatcag ggcgagcggc cccgtgcgcc cgacaatgga ggcggccgac agcacggagc
     1381 gaaggtctgg cgtcaggctc gagatgcggg aggaaacgag cgcctccacg gtgtcgggaa
     1441 tattgatggc accggcctgc gctaccaccg catagctgcc ggcgtcgccg accagaatgc
     1501 cgttgcggct gagatagcgg acggcttcct ccaggaagaa gggcaacccg cccgccatgt
     1561 ccgtcagctt ggctttgacg ggctcgagcg agggatccga ccccagcacc acgcccaaga
     1621 agtcttgcgt ctcttgcgca ctcagttcgc gcaagcgaat ggtgctggcc gttgcaaagg
     1681 catcgaccag cggcgtgtcg ctgccggatc gttcgagcag aatgagcccc gcggacatgg
     1741 cctggatgtg gtgcgcgaat tcggcgagga cctgcagggt ctccgggtca caccactggg
     1801 cgtcatcgac aataataagg agcggccggt gccgcgcctg caaccgcgaa atctccagga
     1861 aacacgcggc caaccggcgc ttgcggtcga tcaggtcgta atttgcccag tccgtgccgg
     1921 ccacgctgag attgaggagc gccttgaggg cgggtgcgtg ctcggcgagc gtgtcggcga
     1981 actgctgcaa gacggccgat agccgacgct ccacgtcctg ggccgtgttg tggtctgtca
     2041 gcccgagcca gctgcgggcg atggagcgag cgatggcata aggcgaatgc cgcgccggca
     2101 gctcgctctc ggcgaaccag atggtgcaat catcgacaag gccggaggtc agggcgtcgt
     2161 gaataaagcg cgactttccg gtcccggggt caccgctgac gctgagcacg ccaccgaggc
     2221 cttgcttggt ttgctcgatg aaagacttga gtagcgcccg ttccggcgcg cgcccgcaat
     2281 agggcgtcag ccccatcagg gcgcgggtat tccagcggga gcggtgctcg acgtcgagca
     2341 gctcccaggt ttccacaggc tggtcgaacc ccttgaaggt aaagcgtccc cggggttgca
     2401 cctcggcggt ggtggcaacc gcacggtaaa cgtcttccga gatcaggatg gtattgggtt
     2461 cggctgccgt ctcgagccgg ctggccagat gaaccgtggc gccgatcatg gaattaccgg
     2521 cctcgccggg cgtgacgatg acctcgccgg tgtgaatgcc aatgcgcacc ggcaggcccg
     2581 gcgaagcacc gcctttggcg gcttggcgaa tggcgagcgc ggcccggcag gcggcgagcg
     2641 gatgctcctc caggctgtcc aaggcgccga agccggacag aataccatcg cccctgactt
     2701 caatgacggt gccgccctgc tcgcgcacgg cgccggccat ggcgttgatc atcggtctga
     2761 tgcgcagcat ggccgcctca gcgtcgatgg cgctgatggc cttggtcgat cccttgatgt
     2821 ccgcgaacag gacggtcgcg accttgcgcg aggcctgggc agccgcctgg ctcgcgctct
     2881 tggcctccaa gggcgtgccg cactcgctac aaaatttgcc cgtcggactg gacaatccgc
     2941 agcgcgggca gtgcatcccc ggtcgtgccg acattgtttg ccgttaccgc gttacgcggg
     3001 tttggttttt tggcgtgcct atagtttaaa acgtcattcg tccgtcaacg ccaaaggcta
     3061 tttgctgacg aggtaaaatc ggccggcgca ctatagaccc aacattgggg ccggatgtgc
     3121 tgcagatcac gggccacaca aagttttgac ggcggatctg tgaccgacag ggcgac
//
LOCUS       DASTMT010000472         2060 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_6044405, whole genome shotgun sequence.
ACCESSION   DASTMT010000472 DASTMT010000000
VERSION     DASTMT010000472.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2060)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2060
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6044405"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            516..1718
                     /locus_tag="VFR00_10885"
     CDS             516..1718
                     /locus_tag="VFR00_10885"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0159813.1"
                     /translation="MMAERRVLFWMCVLVAINQLGFGAMVPSLPLYAQSFGVPTSAIG
                     MAVAAYGLARFVLAVPSGQLADWLGRRPTLAIGGLVSAIGNLCCAWANAYPEFVVARF
                     VAGAGAGLILTTGQVVLADISSPAQRGRSIAIYQGTFLFAVGIGPFPGGLLAEHFGLA
                     APFVAYGGASLLAAIVAWFAVSETRDLWRAAAPTEAAPRPPLAHQLRLLAGKIGFVLV
                     SLISLMNAVVRTGGLFAIVPVLATTLLGLSVGAIGFALMLGSICGLFAAYPAGWLADR
                     FGRKIVIVPATLMTATSMLLFCVAPNYGWFIAAAVVWSVASSVGGAAPATYAADSAPL
                     GMNATAMSAFRMTADAGYVIGPLLLGLIADLYGPVVSLLSAAFLLLLVGALFALLAPE
                     THAGRRIP"
     gene            1832..>2060
                     /locus_tag="VFR00_10890"
     CDS             1832..>2060
                     /locus_tag="VFR00_10890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0159814.1"
                     /translation="MSSPARSRLGLLVVGLGTLLAPLDTAVNIAFPSITQAFALHLED
                     IRWVVVAYVLTYASLMLAFGRLGDLVGYRPIF"
BASE COUNT          247 a          743 c          658 g          412 t
ORIGIN      
        1 cggtccttgc tgcctagcgc ccctggcgcc acctcacgaa cggcgacact agcctaacaa
       61 gtgtggcctc gaccactcgg ctgccgcggg ctcgccgggg gccgaaggtg ctgcgcgcgc
      121 ctgtccaagt tcgcccaggc gcaggcccga gccgccccgc ggccccccag gcggccggct
      181 cgctagggcc ggacgtggcc ccgggtccga gacggcgaga gcgcgctgct gccgacggcc
      241 tgccgggctg gaccgggcgc gatgggggcg ggtgcgcgcc cgtgttgccg cgttgccccg
      301 ttcgcaacca gacgcgcgcg ccatggccgg cgatgctcgg cttttgatgc gccttgcgct
      361 ctcaacggcc gcacccacat ggcccgttgc ggcattgccg gccattgccg tcggcaaggc
      421 cttggcctac agcattaaaa agccaagtcg atccagcgct cgcgcgtcct cccaacaccg
      481 accctagcgg caacaggtcc gacagtctgc gccccatgat ggctgaacgc cgcgtgctct
      541 tctggatgtg tgtgctcgtc gccatcaatc agctaggctt cggcgccatg gtgccgtcgc
      601 tgccgctata tgcgcagtcg tttggggtac ccacatccgc catcggcatg gccgtggccg
      661 cctatggttt ggcgcgcttc gttttggcgg tgcccagcgg gcagctcgcg gattggttgg
      721 ggcggcgacc aacgcttgcc atcggcggcc tggtttcggc catcggcaac ctgtgctgcg
      781 cctgggccaa tgcctatccg gaatttgttg tcgcccgctt cgtggcgggg gccggcgccg
      841 gcctgatcct caccaccggc caggtggtcc tcgccgacat ctccagcccc gctcagcgcg
      901 ggcgcagcat cgccatctac cagggcacat tcctgttcgc ggttggcatt ggtccgtttc
      961 ccggcgggct gctcgccgag catttcgggt tggcggcccc gttcgtcgcc tatggcggtg
     1021 ccagtctgct tgcggccatc gtcgcctggt ttgccgtcag cgaaacccgc gacctgtggc
     1081 gcgccgccgc tccgaccgag gccgcaccgc gcccgccgct tgcccaccag cttcgcctgc
     1141 tggccggcaa gattggcttc gtcttggtca gcctgatctc cttgatgaat gcggtggtac
     1201 gcaccggcgg tctgtttgcg attgtgccgg tgctggccac gaccctgctt ggcttgtcgg
     1261 tgggtgccat cggctttgcc ctcatgctcg ggagcatctg cggtcttttt gccgcctacc
     1321 ccgccggctg gctcgccgac cgcttcggcc gcaagattgt catcgtgccg gcaacgctga
     1381 tgacggccac ctccatgctg ttgttctgcg tggcgccgaa ctacggctgg ttcatcgccg
     1441 ccgcagtcgt gtggagtgtg gcgagttccg ttggcggagc cgcgccggcg acctatgcgg
     1501 cggactccgc cccgctcggg atgaatgcca ccgccatgag cgcgttccgt atgaccgccg
     1561 acgccggcta cgtcatcgga cccttgctcc tagggcttat cgccgacctc tacggcccgg
     1621 tcgtttcgct gctgagcgcg gcatttctgc tgctgctggt gggcgcccta ttcgcactgc
     1681 tggcgccgga aacgcacgcc gggcgcagga tcccctaacc cacagccgtg gcggggccgc
     1741 ccgcgtcttc gcgcgcatac cgctgcgcga tgggtatgag atagggtatc ggtgtcctgc
     1801 gtcgaacctc agcccgcggc ttttctcggc aatgtcgtca ccagcacgat cacgtctcgg
     1861 cctgctcgtc gtcggcctcg gcacgctatt ggcgcccctc gataccgccg tcaacatcgc
     1921 cttccccagc atcacgcagg cctttgccct ccaccttgag gatatccgtt gggtcgtcgt
     1981 cgcctacgtc cttacctacg ccagtctcat gttggccttc ggcaggctcg gcgatctcgt
     2041 cggctaccgg ccgatctttc
//
LOCUS       DASTMT010000473         3059 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_3550028, whole genome shotgun sequence.
ACCESSION   DASTMT010000473 DASTMT010000000
VERSION     DASTMT010000473.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3059
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3550028"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..375)
                     /locus_tag="VFR00_10895"
     CDS             complement(<1..375)
                     /locus_tag="VFR00_10895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0159815.1"
                     /translation="MRKLNALASAFGLLGLQQALAADEAAPEALPRPQWSVLVSFSPT
                     YTNNAQFNRDDRRREVYAEPDVMLRLDGKLTSDISYRLYGRTSFDAFSQEQIANETFA
                     LVGARLTRNILDWRTSVSFEHRY"
     gene            1367..1996
                     /locus_tag="VFR00_10900"
     CDS             1367..1996
                     /locus_tag="VFR00_10900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009489697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU0159816.1"
                     /translation="MARILIVDDQYLVRVGIRRILRAQPWLKVVGEAGDGEEAVAKAI
                     ETKPDVILLEYLLPLITGLEVVRRIRAQRPETEFLMFTLHDNEVLIEQALKAGVRGYL
                     FKSDPSRDLIAALMALASHQPFFTSKVSMLLLEALLATPDEGAIITNRQRCIVQLIAE
                     GYQTKQIAKILGIGLKTVETHRAHIMHRLELSSSAALVRYAVRARLAEA"
     gene            complement(2024..2932)
                     /locus_tag="VFR00_10905"
     CDS             complement(2024..2932)
                     /locus_tag="VFR00_10905"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU0159817.1"
                     /translation="MAGTLQKPDAAQRTVGVIGLGLMGGAMAGNLVAGGWRVVGYDID
                     AKAIAAARRKGIEVVEDAAAVAAAASDIITSLPSARAAIATAETIAASARTGLVVMEM
                     STLSLDDKETFAARLTAKGHIALDCPLSGTGAQAETKDLVVYASGNSREIERLAPLFL
                     GLGRRYFDLGAYGNATKMKFVANLLVAIHNVASAEAMVLGMKAGLAPHQIVDVIAAGA
                     GTSRVFELRAPLMADNRYEPASMRCSIWQKDMAVIGAFAASLGCPTPLFSATEPIYRS
                     GLEHGHGAHDTASVCAVLENMAGLKR"
BASE COUNT          542 a         1018 c          973 g          526 t
ORIGIN      
        1 gtagcgatgc tcgaagctga cgctggtgcg ccaatccaag atgttccttg tcaggcgcgc
       61 gcccaccagg gcaaaggtct cgttggcaat ctgctcttgc gagaaagcat cgaaggaggt
      121 gcggccatag aggcgatagg aaatgtccga cgtcagcttt ccatccaagc gcagcatcac
      181 gtccggttcg gcgtagacct cgcgccgccg gtcgtcgcgg ttgaactggg cattgttggt
      241 gtaggtcggg gagaaagaaa cgagcacgct ccattgcggc cgcggcagcg cctcaggcgc
      301 ggcctcgtcg gcggcgagtg cctgttgcag tcccagcaac ccgaaggcgc tggccaaagc
      361 attcagcttc cgcaccaaaa tcagtcctca gctcgtgagg agaaatttcc gaccggcacc
      421 gcggttgact gacgacgcgc gaaaagaggg gaaaagcgcg atggcaacaa aacgactcaa
      481 agctatgggc tgtcgactct aagctgggct tggcgccgaa gccaagatgc tttttggctc
      541 cgtccaccgt ttttgcagtg gctgccgccg ccacccgctc cgggcactcg ccccgccacc
      601 cgccccggca ctcgcgatga cgcgcgcgaa ggctttgagc ggctgcccat gccgccggcc
      661 ccgcgcacat cgcgcgggca tcgggcccat tcagcgcaat cctcggtgaa cggcagccgg
      721 cgacattcag ggttctcatg catgtacaag acaccatcgc cccgcgcaca ccttctttgc
      781 atcgccctcg cgggcgtgcc gacccgccag cccgtgtgac cgcacattct agagcaaagg
      841 agagtggagc catgtccgac ctcaaccgcc ggagcatcct cggagccatg ctaggtggga
      901 tcgccgtcgc cggtggcgcg agcctcttgg ttgcaccaac aacgcacgcc gctctgctgc
      961 cgggcgttgc cgccacgccg cccggccagg gccccgtcga aaaggcgcaa gtcgttgtgg
     1021 tcggaccgcg tcggcggcgg cggcgctggc gctgctggtg gcgccgcggc cgtcgcgtgt
     1081 gcggctggcg ctggtagccg cccgacacct ttgcgcgagc cccgacacct ttgcggagcc
     1141 caggagaccc tcggcgggcc ggcgccttgc ggcgaagatc cgacgcccgc cccaccagcc
     1201 ccagctgatg ccagcgctta ggcgcggacg agccggagct tgggcaaccg cgcggcaaag
     1261 ggtgcgcgcg tgtccgtatc ggggtttccc tcgatgtcaa attgggcaca aaggcggcaa
     1321 gaatgactga gccgcaacga aagggggtga aagatcagcg cgtgtcatgg cacgcatact
     1381 tattgtcgac gatcaatacc tggtccgggt cggaattcgt agaattcttc gggcgcagcc
     1441 gtggctgaag gtggttggcg aagccggtga tggcgaagag gccgtcgcca aggccatcga
     1501 aaccaagccc gatgtgatcc tgctcgaata cttgttgccc ctcatcaccg gcttggaggt
     1561 cgtgcgccgc atccgcgccc agcggcccga aaccgaattt ctcatgttca cgctgcatga
     1621 caatgaggtc ctgatcgaac aggccctgaa ggcgggcgtg cgtggttacc tgttcaaatc
     1681 ggatcccagc cgtgacctga tcgcggccct gatggcgctc gccagccatc agcctttctt
     1741 taccagcaag gtctccatgc tcttgctcga ggcgctgctg gcgacgcccg atgagggcgc
     1801 catcatcacc aaccgacagc gctgcatcgt gcagttgatc gccgaaggct atcaaaccaa
     1861 acagatcgcc aaaattctcg gcatcggcct caagaccgtg gagacgcatc gcgcgcacat
     1921 tatgcaccgg ctcgaacttt cctcatcggc ggccctggtg cgctatgccg tgcgggcgcg
     1981 gcttgcggag gcatgagcgt cctggtctgc ggcgcggcgc cggtcagcgt ttgaggccgg
     2041 ccatgttctc gagcacggcg cagacggagg ccgtatcgtg ggcgccgtgt ccatgctcga
     2101 gacccgagcg atagatcggc tcggtggcgc tgaacagggg cgtcgggcag ccaaggctgg
     2161 ccgcaaaggc gccaatgacg gccatgtcct tttgccaaat cgagcagcgc atgctggcgg
     2221 gctcgtagcg attgtccgcc atcaacggcg cgcgcaactc gaacacgcgg gacgttcccg
     2281 cgcctgccgc aatcacgtcc acgatctgat gcggcgccaa tccggctttc atgccaagca
     2341 ccatcgcctc ggcgctggcg acgttgtgga tcgcaaccaa caggttggcg acgaacttca
     2401 ttttggtggc gttgccgtaa gcgccaagat cgaagtacct gcggccgagg ccaaggaaga
     2461 ggggagccag gcgctcgatt tcgcggctgt tgccgctggc atagaccacg aggtccttgg
     2521 tttcggcctg cgcccccgtg cccgacaacg ggcagtccag cgcgatgtga cccttggccg
     2581 tcagccgcgc cgcaaatgtc tccttgtcgt ccagggacag cgtgctcatt tccattacga
     2641 ccaggccggt gcgcgcgctc gcggcaatcg tctcggccgt ggcgatggcc gcccgcgcgc
     2701 ttggcagcga agtgatgata tccgaggcag cggcggcaac ggcggccgca tcctcgacca
     2761 cctcgatgcc cttccgcctg gccgcggcaa tcgccttggc gtcaatgtca tagcccacga
     2821 cgcgccagcc accggccacg agattgccgg ccatcgcccc gcccatcagg ccgagaccga
     2881 taacgccgac ggtgcgctgc gccgcatcgg gcttttgcaa tgtgccggcc atcctgctct
     2941 cctgctgccg ctcgcctcag tgcgctcggc cttttgccca tgctaccgat agcagcacac
     3001 cttggacaga tcggaaccgc cggcgaactc gttgccggcc gatcaggcat tggctgtcg
//
LOCUS       DASTMT010000474         1981 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_268725, whole genome shotgun sequence.
ACCESSION   DASTMT010000474 DASTMT010000000
VERSION     DASTMT010000474.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 1981)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1981)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1981
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_268725"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            316..1965
                     /locus_tag="VFR00_10910"
     CDS             316..1965
                     /locus_tag="VFR00_10910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0159818.1"
                     /translation="MQRLPARPNLTHLKKQAKDLLLHHRRGDPAALARLRAALPDGAS
                     NDDPGFVNLGLQQAQWCLAREYGFASWADLKGFVDARSQPSAGDTLLRWLRLAYAADI
                     AGGLDRARPLLAARMLAESEGLLGEDPYLACVIGEEAILRRATARDPGWLKRPGGPLA
                     LPPLLALTHSTFLQLAGFRARMHVCAHLLLQSGADPNQAVGSRWPPASLQAPSADFML
                     SPLYGAAGRNHDPALTQMLLEAGADPNDGESLYHALDDLECTRRLLLAGATISGTILY
                     RALDLDNLEVLRLLLACGADPNEPPPGPPTADWGSPLLWAIRRRRSPAQIAALLDAGA
                     DPAARTPDGASAYTLAQRFALAEVAGRLSRADRAEPVSDEERFLAACARADEATARRL
                     QAERPDFPAALNQAQLRLLPELAAQGCNAAVMLMVRLGWPIAIRGGDWSASALNLAVF
                     RGDAQLTRFLLAHGASWREEHGHGDNVCGTLSWASCNEPVAGGDWPGCAEALTVHGFP
                     AAELDAEAGNGVIIDARRKVFSDAVADVLLAGRQNGASATA"
BASE COUNT          295 a          673 c          652 g          361 t
ORIGIN      
        1 caacgtcgtg cagcacgggg gtatgtcgcg gccagcccag tggaactggc cgcgacaggt
       61 cccaattcca atgtcaattc agaccggggc cggcctattg tttgccgtct tgtgcgcaag
      121 cctcgcgctg tgacggcgcg ctttgcttac gcgcgttgac cattgtccgg catttgccgt
      181 agtgttcggg cggcggagca agccgccacc ggacccgcgg gaagggttga acccgcttgc
      241 agcgacaagg ctgacatttg cacctttgtg cagccgaatt ggcgggtcgt cggcgctttg
      301 tgcacggagg tcctcatgca gcggctcccc gcccggccga atctcacgca tctgaaaaag
      361 caggcaaaag acctgctgtt gcaccatcgc cgtggcgatc ccgctgcgct tgcccgcctg
      421 cgcgctgcct tgcccgacgg cgccagcaac gacgatcctg gttttgtcaa ccttggcctg
      481 cagcaggcgc agtggtgcct ggcccgcgaa tatggctttg cctcgtgggc ggatttgaaa
      541 ggctttgtcg acgcgcgctc tcagcccagt gctggcgaca ctctcttgcg atggttgcgc
      601 ttggcctatg ccgccgacat tgccggtggg ctagatcgcg cccgcccgct gctggcggcg
      661 cgcatgctgg ccgagagcga aggccttctt ggcgaggacc cttacctcgc ctgtgtgatc
      721 ggggaggagg caatccttcg ccgagcgacg gcgcgcgatc ccggctggct caaacggccc
      781 ggcggcccgc tcgcgctgcc gccgcttctg gccctgacgc attcgacgtt tttgcagctc
      841 gctggctttc gcgcgcgcat gcacgtttgc gcgcacttgc tgttgcagag cggcgccgat
      901 cccaatcaag ccgtcggtag tcgctggccg ccggcctcgt tgcaggcgcc gtccgcggat
      961 ttcatgctct cgccgcttta tggtgcggct ggccgcaacc acgatccagc gctgacgcaa
     1021 atgctgctgg aagctggggc ggaccccaac gatggagaat ctctctatca cgccctggac
     1081 gacctcgagt gcacacgtcg gctgttgctg gctggcgcca cgatttcggg cacgatcctt
     1141 taccgtgcgc tggatctcga caacctcgag gtcctgcggc tgctgctcgc gtgcggcgcg
     1201 gacccgaatg aaccgccccc cggtccgcca accgccgatt ggggctcgcc gctgctttgg
     1261 gcaatccgac ggcgacgatc gccggcgcag atcgcagcgc tgctcgatgc cggagccgac
     1321 cccgccgcaa ggacgccgga cggcgccagt gcctacacac tggcgcaacg gttcgcgttg
     1381 gcggaagtgg cgggccgtct gagccgcgcc gacagagccg aaccggtcag cgacgaggag
     1441 cggttccttg ccgcttgcgc ccgggcggac gaggcaaccg cccggcgcct ccaagccgag
     1501 cggcccgact ttcccgccgc gctcaaccaa gcccagctgc gcctactgcc ggaactcgcc
     1561 gcccaaggct gtaatgcggc cgtgatgctt atggttaggc ttggctggcc gatcgcaatt
     1621 cgcggcggcg actggagtgc ctcggccctc aaccttgcgg tgtttcgcgg cgacgcccaa
     1681 ctcacccgct tcctgctcgc acatggcgcg agttggcggg aagagcacgg tcacggcgac
     1741 aatgtgtgtg gcacattgtc ttgggcctcg tgcaacgagc cggttgctgg cggcgattgg
     1801 ccgggttgcg ccgaagcgct cacggtccat ggctttcccg ccgccgagct agatgctgag
     1861 gcaggcaacg gcgtgatcat cgacgcacgc cgcaaagtgt tctccgacgc ggttgccgac
     1921 gttttgctgg ccgggcggca gaacggcgcg tccgccaccg cctagcccca aggggcaaaa
     1981 a
//
LOCUS       DASTMT010000475         3501 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_3551225, whole genome shotgun sequence.
ACCESSION   DASTMT010000475 DASTMT010000000
VERSION     DASTMT010000475.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3501
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3551225"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..238)
                     /locus_tag="VFR00_10915"
     CDS             complement(<1..238)
                     /locus_tag="VFR00_10915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0159819.1"
                     /translation="MLRVGAIGAVMLGLAATAGAGNLASLEARAGDWTRYHNERFGFG
                     LEYPANLFRVERTTEAGDGRVFAAREGEARLLVGA"
     gene            complement(380..1168)
                     /gene="xth"
                     /locus_tag="VFR00_10920"
     CDS             complement(380..1168)
                     /gene="xth"
                     /locus_tag="VFR00_10920"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014285794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEU0159820.1"
                     /translation="MRIATWNINGAKARLEGALTWLKETNPDVACFQEIKSSNEAFPA
                     AFFEELGYHVAVHGQKGFNGVAVLSKRRFDEVVVRGLPGDDGDAHARYIEVMVPNGSG
                     IVRIGNLYLPNGNPIGTDRFAYKLAWMRRFEARVRELLGLEEVFLLMGDYNVIPEPAD
                     AKNPDAWHEDALFQPETRAAYRALINLGLTDAVRACQPEAGIFTFWDYQAGAWQKDHG
                     IRIDHILLSPQAADRLLGAGIDKFTRGWEKPSDHVPVWVELQDG"
     gene            complement(1284..1643)
                     /locus_tag="VFR00_10925"
     CDS             complement(1284..1643)
                     /locus_tag="VFR00_10925"
                     /inference="COORDINATES: protein motif:HMM:NF017837.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF930 domain-containing protein"
                     /protein_id="HEU0159821.1"
                     /translation="MAAIVLVTPAGAQTALDKRMQALEPHTRMIQLCNLSGLESFAKD
                     KKVGKVDRVRIDALAQPTIESNIAQGAGGAVRTAGHWYKFSYRCVLTHDRTRATHFSY
                     VLEREIPKAQWEKFNLF"
     gene            complement(1827..2159)
                     /gene="erpA"
                     /locus_tag="VFR00_10930"
     CDS             complement(1827..2159)
                     /gene="erpA"
                     /locus_tag="VFR00_10930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007815592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HEU0159822.1"
                     /translation="MEHPNVTVSDRAARRIAEIVAGQPDKPLLRVSVEGGGCSGFQYK
                     FDLVGHSEADDLVIEHGGARVLIDRMSLEYMAGSEIDFVDDLIGSAFKVINPNATASC
                     GCGTSFSI"
     gene            2326..>3501
                     /locus_tag="VFR00_10935"
     CDS             2326..>3501
                     /locus_tag="VFR00_10935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HEU0159823.1"
                     /translation="MAKAMIEEPAGVGERRLPAGRALAPYATRPELSTGRLYTEPACP
                     MRTPFQRDRDRIVHATSFRRLTYKTQVFIFHEGDHYRTRLTHSLEVAQIARAIARLLW
                     LDEDLAEALALAHDLGHPPFGHAGERALAAAMAAWGGFDHNAQSFKVVTRLERKYASF
                     DGLNLSFETLEGLAKHNGPLPEAALTAPTLRRAIAESGVENKLALPLFAPAEAQVAAI
                     ADDIAYDSHDLDDGLRAGLLSIEELATVPLVGRFLDDVPRKNIDSQRLIYEINRRMIT
                     AMIDDAVAESRRRLAALQQASLDGVRRADRAMVAQSACVGAQAEALKAYLYANVYRHP
                     RVMRIMRDAEAIVGDLFARYLRQPEEMSGALGEAGRAAGEGSRAGIVADFVAGMTDRY
                     "
BASE COUNT          588 a         1165 c         1140 g          608 t
ORIGIN      
        1 gggcgccgac caggagccgc gcctcaccct cgcgcgccgc gaagacccgg ccatcgccgg
       61 cctcagtcgt ccgctcgacg cggaagaggt tggccggata ctcaaggcca aagccgaagc
      121 gctcgttgtg atagcgtgtc cagtccccgg cccttgcctc gagggacgca aggttgccgg
      181 cccctgcggt ggcggccaat cccagcataa cggccccgat agccccgacc cgcagcatga
      241 cgcaaactcc tcaacctcgt agcggatcaa ccgcgacggg gaggtcctgg ttccggcttt
      301 ggcgccgccg cgcccccgag cccaagaagg cgcgcggcgc cggccgcgcc gacgcaccga
      361 gccgggcgcg cctggcccgc tagccgtcct gcagttcgac ccagacgggg acgtgatctg
      421 agggcttttc ccagccgcgg gtaaacttgt cgatgccggc gccgagcagt cgatcggccg
      481 cctgcggtga cagcaaaatg tggtcgatgc gaatgccgtg gtccttctgc caggcgccgg
      541 cctggtagtc ccagaaggtg aagatgccgg cctcgggctg gcaggcgcgc accgcatccg
      601 tcagccccag attgatgagc gcacggtagg ccgcgcgcgt ctccggctga aacaaggcgt
      661 cctcgtgcca ggcgtcgggg tttttggcgt cggcgggttc cgggatgacg ttgtaatcgc
      721 ccatcagcag gaacacctct tccaggccca gcaattcccg gacgcgcgcc tcaaagcgcc
      781 gcatccaggc gagcttgtag gcaaagcggt cggtgccgat tggattgccg ttgggcaggt
      841 agagattgcc gatgcgaacg atgccgctgc cgttcggcac catcacttcg atgtagcgcg
      901 cgtgcgcatc gccgtcgtcg cccggcaagc cgcggaccac gacctcatcg aagcggcgtt
      961 tgctcaagac ggcaacgccg ttgaagcctt tctgcccatg caccgccacg tggtagccga
     1021 gctcttcgaa gaaagcggcg gggaaggcct cgttgctgga cttgatctcc tggaaacagg
     1081 cgacgtccgg attggtctcc ttgagccagg tgagggcgcc ctcgagccgg gccttggcgc
     1141 cattgatgtt ccaggtggcg atgcgcatgc ccctgttgca caccagcgcg ccgctgcggt
     1201 caagcggcag gcagatacgc gcggcctatg cgtccgaccc ggatttggcc gcgcagggcg
     1261 gcatcaagtc cactccaggg tcgtcagaac aggttgaact tttcccattg tgccttggga
     1321 atttcgcgct ccaacacgta gctgaagtgg gtggcgcggg tgcggtcgtg cgtcagcacg
     1381 caacggtagc tgaacttgta ccaatgtcct gccgtgcgca ccgccccgcc ggcgccctgg
     1441 gcaatgttgc tctcaatggt cggctgcgcc agggcgtcga tgcgcacgcg atcgactttg
     1501 cccactttct tgtccttggc gaagctctcc agaccggaca gattgcacag ctggatcatt
     1561 cgtgtgtgcg gctcgagcgc ttgcatgcgt ttgtcgagcg cggtttgggc gccggccggc
     1621 gtgaccaaga cgatggcagc caagcctatc caggcgctca atccctttgt gcgcaacgtc
     1681 tttccccctc tggctcaacc tcgccgcgcg gtgaggcgcg acccccaggc gggaaagacg
     1741 ctgaagatgc ggtgaaaaag tggcggccct ggcccggcgc tcggcctggc gagcccgtgc
     1801 caggccgcct ctccgcttct cggcgctcag atggaaaaac tggtgccgca gccgcacgat
     1861 gcggtcgcgt tgggattgat taccttgaag gcggagccga tcagatcgtc cacgaagtcg
     1921 atctcggaac cggccatgta ctcgagcgac atgcggtcga tgagcacgcg cgccccgccg
     1981 tgctcgatga cgaggtcatc ggcctccgag tgtccgacca ggtcgaactt atattgaaat
     2041 cccgagcagc caccgccttc gacactgacg cgcaacaacg gcttgtcggg ctggccggcg
     2101 acgatctcag caatccgccg cgctgcccga tccgacacgg taacgttcgg atgttccatc
     2161 acgttttgca tcacttttta ggcatcggtt gcggcccgga gcggcctcgg ccctatggta
     2221 ggggccatgc cctatcctgg cacggcccgt gccctatgct gaggcacgat tcttagccat
     2281 gaagatagga cggcgcggaa gcttgtcaaa tctcggaacc gacgcatggc caaggcgatg
     2341 atcgaggagc cggcgggggt gggcgagagg cgcctgccgg ccgggcgcgc gctggccccc
     2401 tacgccacgc gtcccgagct gagcactggg cggctttaca ccgagcccgc ctgtccgatg
     2461 cgtaccccct tccagcgcga tcgcgaccgg atcgtgcacg ccacctcctt ccggcgcctt
     2521 acctacaaga cgcaggtgtt tatctttcat gagggcgacc actatcgcac ccgcctgacc
     2581 catagcctgg aagtggcgca gatcgcgcgc gccatcgcgc ggctgctctg gctcgatgag
     2641 gatctcgccg aagccctggc cttggcccat gacttggggc atcccccctt tggccacgct
     2701 ggagaacggg cgctggcggc ggccatggcg gcctggggcg ggttcgacca caacgcccaa
     2761 agtttcaagg tggtgacgcg gctcgagcgc aagtatgcga gtttcgacgg gctgaacttg
     2821 agctttgaga cgctcgaggg cttggccaag cacaatggac cgttaccgga ggcagccctg
     2881 acggcgccca cgctgcgccg ggcgattgcc gaaagcgggg tggagaacaa gctcgctttg
     2941 ccgctgtttg ctcctgccga agcccaggtt gcggccatcg ccgacgatat tgcctatgat
     3001 agccatgacc tcgatgatgg cctgcgcgcc ggcctgctca gcatcgagga gttggcgacg
     3061 gtccccctgg tcggccgctt tctggacgat gtgccgcgca aaaatatcga cagccaacgc
     3121 ctcatctacg aaatcaaccg acgcatgatc accgccatga tcgatgatgc cgtggccgag
     3181 tcgcgtcgcc ggcttgccgc cctgcagcaa gcaagcctcg acggtgtgcg ccgggcggat
     3241 cgcgccatgg tggcccaatc cgcctgcgtt ggtgcccagg ccgaggcgct caaggcttac
     3301 ctgtacgcca acgtctaccg ccatccccgc gttatgcgca tcatgcgcga cgcggaggcc
     3361 atcgtcggcg acctatttgc gcgctatctg cgccaaccgg aagaaatgtc gggcgcactc
     3421 ggcgaggccg ggcgggcggc gggcgagggc agccgcgccg gcatcgtggc cgatttcgtg
     3481 gccggcatga ccgatcgcta c
//
LOCUS       DASTMT010000476         5453 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_6373519, whole genome shotgun sequence.
ACCESSION   DASTMT010000476 DASTMT010000000
VERSION     DASTMT010000476.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 5453)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5453)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5453
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6373519"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1780)
                     /locus_tag="VFR00_10940"
     CDS             complement(<1..1780)
                     /locus_tag="VFR00_10940"
                     /inference="COORDINATES: protein motif:HMM:NF014585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HEU0159824.1"
                     /translation="MTHDRTPLAKSVCDPITLEIVRGALVAIQSEMEAVIERTAMSPF
                     IREKKDFYAALYDAEGRLIAGSNLPVFGDVVGPIAEHYPLASMRPGDIYWFNDCYGSK
                     GAVSHSPDQVFVAPVFAEGELAAFAQSWAHFNDIGGMRAGSLSPDCTEIFQEGIIVPP
                     VRVAREGIIFEELLRVFYRNSRFPEMVKGDTRASMAAIRLGERRLGELFARFGRAKTT
                     SAFAQLLDETAALLRQKLKEVLPEGQHAFTDTIDSDGQGHGPIKLRYMLDVGPERISL
                     DTSRSDDQVPGPVNFLMSPPVPAMIFGSYFLGGSHTLLNAGVERALDDLVVRPGSILQ
                     PTFPAPLGMRGVTMMRNMAACLGLLNVATEGQAMASHSAYVIWYLRGRDAKGELFLLS
                     DGLGVGYGARPHADGNDAVYLVAQENFPSEFLDSVFPVRVIRYAINPDTGGPGRWRGG
                     CGLIREIEVLAPEAMVSMRIDSVEHPPWGVAGGHAAGAGRCIVNPGRPDERILKPLSD
                     GNIVKSGDILRVETGGGGGWGHPFDREAERVAADVRDGFVSRASAEAHYGVVLDDAGK
                     AVDAAGTAERRRRRPPAKLFHRHGYHD"
     gene            1903..3663
                     /locus_tag="VFR00_10945"
     CDS             1903..3663
                     /locus_tag="VFR00_10945"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HEU0159825.1"
                     /translation="MSENGVVQAVSAANVKAHVEHITTEIPSRLAGSANGKRMAEYSA
                     AALTKAGVWARVHEMPGLVSFPEQGELRVLSPVELVIEANTLGHSLPTLPDGLSGELM
                     DVASGAFGEFAGRDATGKITLSELSYHPARHEKQRISALMGATGCVMMNWGHPENTAV
                     PFGSVKPVWGNPTPETLQTEMARLPCIGIARTAGLKLREMLKAGPVRIWMRANVENAW
                     RPVQITVGEIAGRSDDFVVVGGHQDSWPGPQATDNAAGNACILELARVFQQHRDKLRR
                     GLVAGFWTGHETGTMIGSSWFVDRNWDRLREHAVAYLQIDQPACAGTTRWSAASNVEL
                     KRFHQGVERRLLGNRPFAWRRAVKNGDASFFGLGVPMLAAQGAFTEEELKKTALANLG
                     WWHHSIENTIDKLDFEFMADHLRLYAAYLWDLCTAVVLPFEYVSVADAFIERLDELKA
                     GAEGIGLAGASDAARGFKAAAEKLEAVAQDWRQRYATAKTTDEAAAERLNACMKRLSR
                     ALVPLASTSKGAYGHDPYGYTPQGSMIPTLYDVPRYAKLGDGEERWMLETQLVRDRNR
                     IADALGDCRRLIDTTLREIA"
     gene            3682..4410
                     /locus_tag="VFR00_10950"
     CDS             3682..4410
                     /locus_tag="VFR00_10950"
                     /inference="COORDINATES: protein motif:HMM:NF013353.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036361 - racemase activity, acting on
                     amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HEU0159826.1"
                     /translation="MRILYQLTSPMHKTVGEAEVARRRSVLQASAAAGTEVIVEPTAT
                     GPATIESARDAGLVIPELIRLGPLAQQRGHEALIIGCYSDPGLDALRELLSIPVVGPG
                     AASLHLAAQLGTRLSVLTPSGRGFGRVAARLRALGLDALLASVRGIGLSVMDLAKQAP
                     GALDKAAEAGKSAVERDGADVLVLGCMSMAFLPDITARLGERVGVPVVNPVLAALKTA
                     EALVSMRLSHSKQGWPPLKPQEFA"
     gene            4482..>5453
                     /locus_tag="VFR00_10955"
     CDS             4482..>5453
                     /locus_tag="VFR00_10955"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0159827.1"
                     /translation="MSLWRSLSTLAALAALFAVAPPAQAGPPGVLVTGDNLPANVDPH
                     QIFDVPMQFYSLNTYDNLYRYEGNPPKLEPWLAQSHSVSPDGLTWEFKLRTDAKFHDG
                     SPITAEDVVYSFKRVLAIGKGPAGAFKPVLKAENISAPDKETVRFKLETPYAPFLSAI
                     PIVMIVNPRVVQAHEANNDWGAAWLASNAAGSGAYQFDAATYRPLERADLKRNEAHFK
                     GWSDNPKAPKEIQILPTRETSTRVLALLQGSVDMTDSYLPTDQVERIQKSKGVHIEKN
                     TSMRVFILRMNNKKPPFDNIDARKCFAYAFNYDGFIKEILKGYAERNP"
BASE COUNT          984 a         1807 c         1798 g          864 t
ORIGIN      
        1 cgtcgtggta gccgtggcgg tggaacagct ttgctggcgg gcgccgcctt cgccgctcgg
       61 ccgtgccggc agcatccacc gctttgcccg catcatcgag caccacgcca taatgcgctt
      121 cggcgctggc gcgggagaca aagccatcgc gcacgtcggc ggcaacgcgc tcggcctcgc
      181 ggtcgaaggg gtggccccag ccgccgccac cgcccgtctc gacgcgcagg atatcgccgg
      241 attttacgat gttgccatcg ctcaagggtt tgaggatccg ctcgtcgggc cggccaggat
      301 tgacaatgca gcgccccgcg cccgcggcgt ggcccccggc caccccccaa ggtggatgct
      361 cgacgctgtc gatgcgcatg gacaccatgg cctcgggggc cagcacctcg atctcgcgga
      421 tgagaccgca gccgccgcgc cagcggccgg ggccgccggt atcgggattg atggcgtagc
      481 gaatgacccg cacgggaaac accgaatcca aaaactcgga ggggaaattt tcctgcgcca
      541 cgagatagac ggcatcattg ccgtccgcgt ggggccgggc gccgtagccg acgccaaggc
      601 catcggacag caagaagagc tcacccttgg cgtcgcggcc gcgcaggtac cagatcacgt
      661 aggcgctgtg cgaggccatg gcctggccct cagttgccac gttgagcagc ccgaggcagg
      721 ccgccatgtt gcgcatcatg gtgaccccgc gcatgcccaa cggtgccggg aaggtcggct
      781 gcaggatcga gccggggcgc accacgaggt catcgagcgc tcgctcgacg ccggcgttga
      841 ggagtgtgtg tgagccgccc aggaaatagg agccgaagat catcgccggc accggcgggc
      901 tcatcagaaa gttgacggga cccggcacct ggtcgtcgct gcggctggtg tcgagcgaga
      961 tgcgttcggg tccgacgtcg agcatgtagc gcagcttgat ggggccgtgc ccctggccat
     1021 cgctatcgat ggtatcggta aaggcgtgct ggccctcggg cagaacctct ttcaatttct
     1081 gacgcagcag ggccgcggtc tcgtcgagga gttgcgcaaa agcgctcgtt gtcttggcgc
     1141 ggccgaagcg ggcaaagagc tcgcccaggc gccgctcgcc cagccggatc gccgccatgg
     1201 aggcgcgcgt gtcgcctttg accatctcgg gaaagcgcga gttgcgataa aaaacccgca
     1261 acagctcctc gaagataatg ccctcgcgcg ccacccgcac cggcggcacg atgatgccct
     1321 cctgaaagat ctcggtgcag tcgggcgaca gcgaacctgc gcgcataccg ccgatgtcgt
     1381 tgaagtgcgc ccatgactgg gcgaaggccg cgagttcgcc ctcggcgaac accggggcga
     1441 cgaacacctg gtcgggcgag tgtgataccg cccccttgga gccataacag tcgttgaacc
     1501 agtagatatc gccgggccgc atgctggcga gcgggtagtg ctcggcaatc ggaccgacaa
     1561 cgtcgccgaa cacaggcagg ttcgagccgg cgatcaggcg tccctcggca tcgtaaaggg
     1621 cggcatagaa gtccttcttc tcacggatga agggcgacat ggccgtgcgc tcgatcacgg
     1681 cctccatctc gctctggatg gcgacgagcg cgccccgcac gatctcgagc gtgatcggat
     1741 cgcaaacgct cttggctaag ggtgtccgat cgtgcgtcat tcccgctctc gcttgagcct
     1801 ccaaggagcc ctgctcgtga catgccccca agtttgccgc aagatggcgg ggttgaggac
     1861 ccctgaaaaa gcaaagcttg cggccaggtg cgaggaaagc gaatgtcaga gaacggtgtc
     1921 gttcaggctg tgtcggccgc caatgtgaag gcccacgtcg agcacatcac caccgagatc
     1981 ccgtcacggt tggccggctc cgccaacggc aagcgcatgg ccgagtacag cgccgccgcc
     2041 ctgaccaaag cgggggtatg ggcgcgcgtg cacgaaatgc cgggacttgt cagctttccc
     2101 gaacagggcg agctgcgggt cttgtctccc gtcgagctcg tgatcgaggc caacaccttg
     2161 gggcacagcc tgccgacctt gcccgacggc ctctccggcg agctcatgga cgtggcgtcg
     2221 ggggccttcg gcgaatttgc gggtcgcgac gccaccggca agatcacgct gtctgagctc
     2281 tcctaccatc cggcccgcca tgaaaagcag cgcatctccg cactgatggg tgccacgggt
     2341 tgcgtgatga tgaactgggg ccacccggag aacacggcgg tgccctttgg ctcggtcaag
     2401 cccgtgtggg gcaatccgac gcccgagacg ttgcaaacgg aaatggcgcg gcttccctgc
     2461 atcggcattg ctcgcaccgc cggcctcaaa ctgcgcgaga tgctcaaagc gggtccggtg
     2521 cgcatttgga tgcgcgccaa cgtcgagaat gcctggcggc cggtgcagat tacggtcggc
     2581 gagatcgccg ggcgcagcga cgattttgtc gtggtcggcg gccaccagga ctcctggccc
     2641 ggaccgcagg cgaccgacaa cgccgccggc aacgcctgca ttttggaatt ggcccgggtc
     2701 ttccagcagc accgcgacaa gctgcggcgg ggcctggtcg ccggcttctg gaccgggcac
     2761 gagaccggca ccatgatcgg ctcgtcctgg ttcgtcgatc gcaactggga ccgcctgcgc
     2821 gagcacgcgg tcgcctatct gcagatcgac cagccggcct gcgcgggcac cacgcgctgg
     2881 agcgcggcct ccaacgtcga gctcaagcgc ttccaccagg gtgtggaacg gcgcctgctc
     2941 ggcaaccgtc cctttgcctg gcgccgggcc gtaaaaaacg gcgatgcctc gtttttcggg
     3001 ttgggggtgc cgatgctggc ggcgcagggc gcctttaccg aagaggagct gaaaaagacc
     3061 gcgctcgcca acttgggatg gtggcaccac agcatcgaga acaccatcga caagctcgat
     3121 ttcgagttca tggccgatca cttgcgcctt tacgcggcct acctttggga cttgtgcacg
     3181 gcggtggtct tgccgttcga atacgtgagc gtcgccgatg cctttatcga gcgcctggac
     3241 gagctcaagg ccggcgccga gggcattggc cttgccggag ccagcgacgc ggcccggggc
     3301 ttcaaggccg cggccgaaaa gctggaggcc gtcgcgcagg attggcgcca gcgctacgcg
     3361 accgccaaga ccaccgacga ggccgctgcc gagcgcctca atgcctgcat gaagcggctg
     3421 tcgcgggcgc tggtgccgct cgccagcacg tcgaaggggg cctacggcca cgacccctac
     3481 ggctacacgc cgcaaggcag catgatcccg acgctctacg atgtgccgcg ctacgccaaa
     3541 ctcggagacg gcgaggagcg ttggatgctc gagacgcagc tggtgcgcga tcgcaaccgc
     3601 atcgccgatg ccctcggcga ctgccggcgg ctgatcgaca ccacgcttag ggaaatcgcc
     3661 tgacgcaccg gacaaacacc catgcgcatt ctctatcagc tgacgagccc gatgcacaaa
     3721 acggttggcg aggcggaggt ggcccggcgc cggagcgtgc tgcaggcttc ggcggcggcg
     3781 ggcacggagg tcatcgtcga gccgacggcg acgggacctg cgacaattga aagcgcgcgc
     3841 gatgccggcc tcgtgatccc cgaactgatc cgtctgggac cactggcgca acagcgcggg
     3901 cacgaggccc tgatcatcgg ctgctacagc gatcccggcc tcgacgccct acgcgagctc
     3961 ttgagcattc ccgtggtcgg gcccggcgcg gcctcgctgc atctcgccgc gcaactgggc
     4021 acccgcctct cggtgctcac cccctcgggc cgcggttttg gccgggtagc cgcgcgcctg
     4081 cgcgcgctcg gcctcgatgc cctgctggcc agcgtgcgcg gcatcggcct ctcggtcatg
     4141 gacctcgcca aacaggcgcc gggtgcgctc gacaaggcgg ccgaggcggg caagtcagcg
     4201 gttgaacgcg acggcgcgga cgtactggtg ttgggctgca tgagcatggc cttcctgccg
     4261 gatatcacgg cgcggctcgg cgaacgcgtc ggggtgcccg tcgtcaatcc cgtgctggcg
     4321 gcgctcaaga ccgcggaggc cctggtgagc atgcggctgt cgcacagcaa gcaggggtgg
     4381 ccgccgctga aaccccagga gttcgcgtga ggcctgggcc acgcgccgca agagacacaa
     4441 cacgtgtgag accgctcgag acataaaagg agaaaaccgc gatgtcgcta tggagaagtt
     4501 tgtccaccct ggcagcgctc gccgcgctgt ttgccgtggc gccgccggcg caagccgggc
     4561 cgccgggtgt gctggtgacg ggtgataacc ttccggccaa cgtcgacccg caccagatct
     4621 tcgacgtgcc gatgcagttc tatagcctca acacctacga caatctctac cgctacgagg
     4681 gcaatccacc caagctcgag ccctggctcg cccagtcgca cagcgtgtcc cccgatgggt
     4741 tgacctggga attcaaactg cggaccgacg ccaagttcca cgacggcagt ccgatcacgg
     4801 ccgaggacgt cgtctatagt ttcaagcgcg tcctggcgat cggcaagggc ccggcggggg
     4861 ccttcaagcc cgtgctcaaa gccgagaaca tcagcgcgcc cgacaaggaa accgtgcgct
     4921 tcaagctcga gaccccctat gcgccgttcc tatcggcgat cccgatcgtc atgatcgtca
     4981 acccgcgcgt ggtgcaggcc cacgaggcca acaacgactg gggtgcggcc tggctcgcct
     5041 ccaatgccgc cggatccggt gcctatcaat tcgatgcggc gacctatcga ccgctggagc
     5101 gcgccgacct caagcgcaac gaggcccact ttaagggctg gagcgacaat cccaaggcgc
     5161 cgaaggagat ccagatcctg ccgacccggg agacctcgac ccgtgtgctg gcgctgctgc
     5221 agggttcggt cgacatgacc gacagctatc ttccgaccga ccaggtcgag cgcatccaga
     5281 agtcaaaggg cgtgcatatc gaaaagaaca cctccatgcg cgtctttatt ctgcgcatga
     5341 acaacaagaa gccgcccttc gacaatatcg acgcgcgcaa gtgctttgcg tatgccttca
     5401 actatgacgg ctttatcaag gagatcctga agggctatgc cgagcgcaac ccg
//
LOCUS       DASTMT010000477         2007 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_6045591, whole genome shotgun sequence.
ACCESSION   DASTMT010000477 DASTMT010000000
VERSION     DASTMT010000477.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2007
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6045591"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            320..1522
                     /locus_tag="VFR00_10960"
     CDS             320..1522
                     /locus_tag="VFR00_10960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014762479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HEU0159828.1"
                     /translation="MLEPTKPSAARPDIPFDTGRLDRLMEDAGIDLLLATSKHNVQYL
                     LGGHRSIFFDYMDATGITRYLPILAYPKGAPDKAAYIGHRLESHQKEVAPFWTAEALT
                     SSSGSIDATEKAIAYVRKAGLRPKRIGVEMPFLPADAAATLRAGLPDSEITDAVFVLE
                     RLRAKKRPDELALLRRASELVIDAMLAVIGAHGPGTSKRQLFEALRREEVNRGMTFEY
                     CLLTAGTSLNRAPSEQRWEQGDIISLDSGGNYHGYIGDLCRMAIQGEPDAELEDLLAE
                     IETIQRKAMAPIKAGAMGSVIYEAATPLVRQSKNHNHLHFLAHGMGLVSHEAPRLAPR
                     GSMAYDPYDADHPLETGMVISVETTLHHPRRGFIKLEDTVAVTDAGFDIYGEGARGWN
                     RGGTAPAA"
     gene            complement(1541..>2007)
                     /locus_tag="VFR00_10965"
     CDS             complement(1541..>2007)
                     /locus_tag="VFR00_10965"
                     /inference="COORDINATES: protein motif:HMM:NF018352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MipA/OmpV family protein"
                     /protein_id="HEU0159829.1"
                     /translation="GDIDGGLVVGGYAGYRAGAWFFSASYHQQVTGEDTGGLVRLAID
                     HTWRLSPTATLVTSIGTAYASSDYMQTYFGVTAAQSAASLAGLPQYDPSAGFKDVSVG
                     ATATIALDPRWTLYLTGRYSRLIGDAADSPVIETENAFFGGAGLSYKFDLIR"
BASE COUNT          364 a          674 c          657 g          312 t
ORIGIN      
        1 tcagcgtgta ggtgcgaaac tcatagatca tcggcgcttg gccctccctg gcggctaaag
       61 agccggcgga cgttgcggcc gcccgtgccc ccgaccgcca ggatatgcac cttcccccag
      121 gcgccggcaa gtcggctggc ccggcgagcc ccccggtgcc aacgccggcg ccgcggccca
      181 gtgtgtccag agtgcgtcca gagtgcgtcc agggtgcgcc cagggtgcgc ccagtgcatc
      241 cagcggtgca tcgagccttt cgaagcgccg gcggggaagc tatgatttgc gccgaggaag
      301 cgccaacgga gcccacgcca tgctcgagcc caccaaaccg tccgccgccc gtcccgatat
      361 cccctttgac acgggccgcc tcgatcgcct gatggaggac gccggtatcg acctgttgct
      421 ggcgacgtca aagcacaacg tgcagtacct attgggcggc caccgctcca tcttcttcga
      481 ctacatggat gccacgggca tcacgcgcta cctgccgatc ctggcctacc ccaagggcgc
      541 gcccgacaag gccgcctaca tcggccatcg cctggaaagt catcaaaaag aggtggcgcc
      601 gttttggacg gcggaggccc tgacctccag ttcgggttcg atcgatgcca cggaaaaggc
      661 catcgcctac gtgcgcaagg cggggctcag gcccaagcgc atcggcgtcg agatgccgtt
      721 tcttcccgcc gatgccgccg cgacgttgcg cgccggcttg cccgacagcg agatcacgga
      781 tgccgtgttc gtgctcgaac ggctgcgcgc caagaagcga cccgacgagc tggctctgct
      841 gcgccgcgcc tccgaactgg tgatcgatgc catgctggcc gtgatcggcg cccatggtcc
      901 cggcaccagc aagcgccaac tgttcgaggc gctgcgccgc gaggaagtca accgcggcat
      961 gaccttcgaa tattgcctgc tcacggccgg caccagcctc aaccgcgccc cctccgagca
     1021 gcgttgggag cagggcgaca tcatctcgct cgactcgggt ggcaactatc acggctacat
     1081 cggcgacctt tgccgcatgg caattcaggg cgagcccgat gcggagctgg aagacttact
     1141 ggccgaaatc gagaccattc aacgcaaggc gatggcgccg atcaaagccg gagcgatggg
     1201 ctcggtgatt tatgaggcgg caacgccgct cgtgcgccag tccaagaacc acaatcattt
     1261 gcatttcctg gcgcacggca tggggctcgt cagtcacgaa gcgccccgcc tggcgccgcg
     1321 cggaagcatg gcctatgatc cctacgacgc cgaccatcca ctggagaccg gcatggtgat
     1381 ttccgtggaa acgaccctgc accacccgcg gcgcggcttc atcaagttgg aagataccgt
     1441 ggcggtcacc gacgcaggct ttgacatcta tggcgaaggc gcccgcggct ggaaccgggg
     1501 cggcacggcg ccggccgcct gaggcggcgg gtcgacagag ctagcggatg aggtcgaact
     1561 tgtaggacag cccggcgccg ccgaagaagg cgttttcggt ctcgatcacg ggactgtcgg
     1621 cggcgtcccc gatcagccgc gaatagcggc cggtcaaata cagggtccag cgcggatcga
     1681 gtgcgatggt ggccgtggcg ccgacgctga cgtccttgaa gccggcggac gggtcatatt
     1741 gcggcagtcc cgccagcgag gccgccgact gggcggcggt gaccccaaag taggtctgca
     1801 tatagtcgct cgaggcgtac gccgtgccga tactggttac caacgtggcg gtgggactga
     1861 gccgccaggt gtgatcgatg gcaaggcgga ccaagccacc ggtgtcttcg ccggtcacct
     1921 gctggtgata ggaggcagag aagaaccacg ccccggcgcg gtagccggcg tagcctccga
     1981 ccacgagccc gccgtcgata tcgccga
//
LOCUS       DASTMT010000478         6195 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_604570, whole genome shotgun sequence.
ACCESSION   DASTMT010000478 DASTMT010000000
VERSION     DASTMT010000478.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 6195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6195
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_604570"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..768)
                     /locus_tag="VFR00_10970"
     CDS             complement(<1..768)
                     /locus_tag="VFR00_10970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEU0159830.1"
                     /translation="MSDPLKTWGTAMPLPLNKGRLGRGLASLIGEVPSGEQRLPAQGE
                     QRTVGIDQLQRSPLNPRRDFGDEELAELADSIRTKGLLQPIIVRPDAGRGGYEIVAGE
                     RRWRAAQRAGIHQVAVIVRELSDQEVLEVALIENVQRTDLNAIEEATGYNDLTERFGY
                     TQEQLADVIGKSRSHIANTLRLLKLPAAVRTLLAEGSLTAGHARALVGREDAEALAQR
                     IVAQDLNVRAVEALVQSQAKGGDGGGGSGRRGPDKDAD"
     gene            complement(823..1647)
                     /locus_tag="VFR00_10975"
     CDS             complement(823..1647)
                     /locus_tag="VFR00_10975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020187280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEU0159831.1"
                     /translation="MLPGAAALRVLAIANQKGGVGKTTTAINLGTALAAVGQKVLILD
                     LDPQGNASTGVGVAEDARHLTTFSVLSGKASIATAAMPTLVPNLSIVPANSDLVGLES
                     EMMMEANRPFRLRDAVAALIAQQRARPTDVPYTYLLIDCPPSLNLLTLNAMVAAHAVL
                     VPVQCEFFALEGISQLKDTIDQIKSTLNPGLEIQGVVLTMHDARTSLSKEVATEVRAF
                     FGPKVYETMIPRNVRVAEAPSFGKPILLYDYECPGSQAYIRLATEIIEREQRLRAA"
     gene            complement(1653..2384)
                     /gene="rsmG"
                     /locus_tag="VFR00_10980"
     CDS             complement(1653..2384)
                     /gene="rsmG"
                     /locus_tag="VFR00_10980"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEU0159832.1"
                     /translation="MAHPSQDRTGQRRPALTIAGPADFAAAFAVSRETITRLESYAAL
                     LQQWQKAVNLVAASTLDTVWQRHFADSAQLVSLAPEARRWVDLGSGAGFPGLVIAIVL
                     ADAAHAENPAPAAEPGARITLIESDHRKGAFLRAVARQLGVTVDILSTRIESDATQSR
                     VAAPDVVTARALAPLDKLLSLAAPLWSPRTIGLFLKGREAANEVETALKVWTFQHELI
                     PSSTEAEAHIVRVSTLEPNRKEPRS"
     gene            complement(2488..4377)
                     /gene="mnmG"
                     /locus_tag="VFR00_10985"
     CDS             complement(2488..4377)
                     /gene="mnmG"
                     /locus_tag="VFR00_10985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEU0159833.1"
                     /translation="MSSQSYDVIVVGGGHAGCEAAAAAARLGARTALITHSFATVGEM
                     SCNPAIGGLGKGHLVREIDALDGLMGRIADAAGIQFRVLNRSKGPAVQGPRAQADRKL
                     YRQAMQAAIRTIPGLSVIEGAVEDLRVVAAQPAASRLAGVVLADGRSLLAGAAVLTTG
                     TFLRGVIHQGETQIPAGRYGEAPANGLSQRLLALPLRLGRLKTGTPPRLASPSIDWAN
                     LSMQAGDDPPVPFSFLTTRITTAQVTCGITATNAATHALIRANLNRAPLYCGQIGGVG
                     PRYCPSIEDKVVRFADRPSHQIFLEPEGLDDDTVYPNGISTSLPTDVQDALLRTIPGL
                     ARVVVKRFGYAIEYDYVDPRELLPTLELKGLPGLFLAGQINGTTGYEEAAAQGLLAGL
                     NAALRASGAQRDFTLSRADAYLGVMIDDLVTRGVSEPYRIFTSRAEYRLRLRADNADQ
                     RLTPAGLKLGCIGPARAHAFAAKARMLARARQELAALNLTPTEAARHGLDVNRDGRRR
                     SGFELLAYPGITVSQLAAIWPALGALPSWAVAQLEVDARYAAYVDQQDADVSVLRREE
                     AIRLPRDIDYSCICGLSSEVKQRLERARPTTLAQAGRLEGITPAALMLLLSHVKKLPS
                     QRSVS"
     gene            4646..4927
                     /locus_tag="VFR00_10990"
     CDS             4646..4927
                     /locus_tag="VFR00_10990"
                     /inference="COORDINATES: protein motif:HMM:NF016162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF427 domain-containing protein"
                     /protein_id="HEU0159834.1"
                     /translation="MQAKWRGEAVADSDAVRTVGGYVYFPRAAVRMDLLQPSPKTASD
                     LECPHGVQFYNLVRQGATSERAAWSYEAPQAALSSVAHWIGFWKDVELG"
     gene            complement(4970..>6195)
                     /gene="mnmE"
                     /locus_tag="VFR00_10995"
     CDS             complement(4970..>6195)
                     /gene="mnmE"
                     /locus_tag="VFR00_10995"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013950150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEU0159835.1"
                     /translation="PKPRYAAFRRLKHPDTAEVLDHALVLWFPGPASETGEDMAELQV
                     HGGRAVVQGVIAALASLEGCRLAEPGEFARRAFENGKLDLTGVEGLADLIDAETAAQR
                     RQALRQAEGALYRLYEAWRQGLLGAMALTEAGIDFSDEPDVASNAFDEARRQIAPIAE
                     AIAAHLDDDHRGELVRDGFQVVLAGAPNVGKSSLLNALARRDVAIVSEEPGTTRDVLE
                     VKLELAGLAVIVSDTAGIRAAAGKVEEEGIRRALERVRAADLVLWLQDVTDPTAPPPA
                     ADLGVRADRLLIVHNKADLRYGRALPAPTAEALTISALKGYGLETLIERLAAIAAARA
                     GDQEAPALTQARHRQQLLRCRAALSSFLSGPSEDAELRAEDLRRAAQALGRLVGAIAV
                     EDVLEQVFGRFCIGK"
BASE COUNT         1071 a         1992 c         2105 g         1027 t
ORIGIN      
        1 atcggcgtcc ttgtccgggc cgcggcgacc ggagccgccg ccgccatcgc cgcctttggc
       61 ctgactttgc accaaagcct ccacggcgcg cacattgagg tcttgggcaa caatgcgctg
      121 cgccaaagct tcggcgtcct cgcgtccaac cagggcgcgg gcgtgaccgg ccgtgagcga
      181 accctcggcc aacaacgtgc gcaccgcggc cggcaatttc aaaagccgca gcgtattggc
      241 gatgtggctg cggcttttgc cgatcacgtc cgccaattgc tcctgcgtgt aaccaaagcg
      301 ctcggtgagg tcgttgtaac cggtcgcctc ctcaatggcg ttgagatcgg tacgctgcac
      361 gttttcgatc agggcaactt cgaggacttc ctgatcgctc agctcacgga cgatcacggc
      421 gacctggtgg atgccggcgc gctgggcagc ccgccagcgc cgttcgccgg caacgatttc
      481 atagccgccg cggccggcgt cgggtcgcac gataatcggt tgcagcaggc ccttggtgcg
      541 aatcgaatcg gccagttccg ccaactcctc gtcgccgaaa tcgcggcgcg gattgagcgg
      601 actgcgctgc agctggtcga tgcccaccgt gcgttgctcg ccttgcgccg gcaggcgttg
      661 ctcgccgctc gggacctcgc cgattaaaga cgcaaggccg cggccgagcc ggcctttgtt
      721 gaggggtagt ggcatcgcgg ttccccaggt cttaagaggg tcggacacgg tcatcctcgc
      781 tgaaaacggc ggcgcacacc gcctcgattc cgttgttcac gctcaagccg cccggaggcg
      841 ttgctcgcgc tcaattatct cggttgccag ccggatgtac gcctggctgc cggggcactc
      901 atagtcgtaa agcaggatcg gcttgccgaa cgagggtgcc tccgccacgc gcacgttgcg
      961 cgggatcatg gtttcataga ccttgggacc gaagaaagcg cgcacctcgg tggcaacctc
     1021 cttggagagg gaggtccgcg catcgtgcat ggtcagcacg acgccttgga tctccagacc
     1081 gggattgagc gtcgacttaa tctgatcgat tgtgtccttc agttgtgaaa tgccctccaa
     1141 ggcgaagaat tcgcactgga ccggcaccag aacggcgtgc gccgcgacca tagcattgag
     1201 ggtcagcagg ttgagcgacg gcggacagtc gatcaaaagg taggtgtagg gcacgtcggt
     1261 tggccgcgcg cgctgctggg cgatcaaggc ggctacggca tcccgcaagc ggaagggacg
     1321 gttcgcctcc atcatcattt cggattcgag acccaccagg tccgaattcg ccggcacgat
     1381 actcagattt ggcaccagcg tcggcatggc cgccgtcgca atgctggcct tgccactcag
     1441 cacgctgaag gtggtgagat gccgggcatc ctctgcgacc cccacgcccg tggaggcgtt
     1501 gccttgcgga tcaaggtcaa ggatgagcac cttctgaccg accgcggcca acgccgttcc
     1561 cagattgatg gccgtggtcg tcttgcccac cccacctttt tgattggcaa tggcaaggac
     1621 gcgaagcgca gcggctccgg gaagcatgcc agtcacgagc gcggctcctt cctgttgggc
     1681 tcgagcgtgc tgacccgcac gatgtgggcc tccgcttcgg tagagctggg gatcaactca
     1741 tgctgaaaag tccacacctt aagggccgtc tcgacttcgt tggccgcctc gcggcctttg
     1801 aggaacaaac cgatggtacg cggggaccac aacggggcgg caaggctcag cagtttgtcg
     1861 aggggcgcta gggcacgcgc cgttaccacg tccggcgcgg caaccctact ttgagtcgcg
     1921 tcagattcga ttcttgtcga caggatatcc acagtgacgc ccagctggcg ggccaccgca
     1981 cgcaagaagg cgcccttgcg gtggtcactt tcgatgagcg tgatgcgtgc accgggttcc
     2041 gcggcgggcg ccgggttttc ggcgtgggcc gcatccgcca agacaatggc gatgacaagg
     2101 cccggaaaac ccgcccccga gcccagatcg acccaccgtc gcgcctcggg cgcgagggag
     2161 acgagttggg cgctatccgc gaaatggcgc tgccaaacgg tatcgagtgt actggccgcc
     2221 accaggttga ccgccttttg ccactgctgc agcaaggccg cgtagctttc caggcgcgtg
     2281 atcgtttcac gtgaaacagc aaacgcagcg gcgaagtcgg cgggacctgc aatggtcagg
     2341 gccggtcgcc gctgcccggt gcggtcctgg gaagggtggg ccatcggcct cgccatagcc
     2401 gaaaatggag gcgtccggcg caaaatgttt cacgtgaaac acgatccacc cgccttggtt
     2461 gtgccctgga ggggaattgg accttgttta ggagaccgag cgctgagacg gcagcttctt
     2521 gacgtgggaa agcagaagca tgagcgcggc aggcgtaatg ccttctaggc ggcccgcctg
     2581 cgcaagcgtc gtgggtcgtg ccctctcgag gcgttgcttg acctcgctcg agaggccgca
     2641 aatgcagcta tagtcgatat cccgcggcag gcgaatggcc tcctctcggc gcagaaccga
     2701 aacatcggcg tcctgctgat cgacgtaggc ggcatagcgc gcgtcaactt caagctgagc
     2761 gaccgcccag ctcggcagag ccccaagcgc cggccagatc gccgcgagct gggataccgt
     2821 gatgccggga taggcgagaa gctcgaagcc actgcggcgg cgcccatccc ggttgacgtc
     2881 caagccgtgc cgtgcggcct cggtcggggt gaggttcaac gcggcgagct cctggcgagc
     2941 ccgtgccagc atgcgcgctt tggcggcgaa ggcatgggcg cgggctggtc cgatacagcc
     3001 gagcttgaga ccggctgggg tcagccgctg gtcggcgttg tctgcccgca ggcgcaggcg
     3061 atattcggcg cgcgaggtga aaatccggta gggctcggag acccctcggg tgacgagatc
     3121 gtcaatcatc acgccgaggt aggcgtccgc gcgcgaaagc gtaaagtcgc gttgcgcacc
     3181 cgaggcgcgc agggccgcgt taaggcccgc cagcaggcct tgggccgcgg cctcttcata
     3241 gccggtggtg ccgttgatct ggccagcgag gaacagccca ggcaagcctt tgagctcgag
     3301 ggtgggcaga agctcgcgcg gatcgacgta gtcgtactcg atggcatagc cgaagcgctt
     3361 gacgaccacc cgggcgaggc cgggaatggt gcgcagcaag gcgtcctgca cgtctgttgg
     3421 cagggaggtg gagatgccgt tggggtagac cgtatcgtcg tcgaggccct cgggctcgag
     3481 aaagatctga tggctgggcc ggtcggcaaa gcgcaccacc ttatcctcga tggaggggca
     3541 gtagcgcggg cccacgccgc cgatttggcc acagtaaagc ggtgcccggt tgagattggc
     3601 gcgaatcagg gcgtgtgtgg cggcgttggt ggcggtgatg ccacaggtca cttgcgcggt
     3661 ggtgatacgg gtggtgagaa aggaaaaggg gaccggggga tcgtcgcccg cctgcatcga
     3721 caggttcgcc caatcgatgg acgggctggc caagcggggc ggcgtgccgg tcttcagccg
     3781 cccgagccgc agcggcaagg ccagcaaccg ctgcgaaagg ccgttggctg gtgcctcgcc
     3841 gtagcgcccc gccggtatct gcgtctcgcc ttggtggatc acgccgcgca aaaacgtacc
     3901 ggtggtgaga acggccgcgc ccgccaaaag gctgcggcca tccgccagca cgacgccggc
     3961 cagacgggag gctgccggtt gcgcggcgac aacacggagg tcttcgaccg cgccttcgat
     4021 gacactgagg cccgggatgg tccggatggc ggcctgcatg gcttgccgat agagcttgcg
     4081 gtcggcctgg gcgcggggac cttggacggc gggccccttg gatcggttga gcacccgaaa
     4141 ttggatccca gcggcatcgg cgatgcgtcc catgaggcca tcgagggcgt cgatctcgcg
     4201 caccagatgc cccttgccga ggccgccaat ggcgggattg cacgacatct cgccaacggt
     4261 agcaaaactg tgggttatga gcgccgtgcg cgcgccgagg cgagcggcgg cggcggcggc
     4321 ttcgcacccc gcgtgaccgc cgccaacaac gatgacgtca tagctctgcg aagacatgga
     4381 tttggggcgc catggcggct ggaccatccg gccgcagacc ggcgatgcgg cgcaacctac
     4441 tggagccgcg ccacggggtc aaagcccgcg ttgcagccga gcgccggcag acctatctga
     4501 ggggggcgcc tcgacgaacg tgatcgggag cgcatccgag gtctgcccgt caggaggttg
     4561 gccgaggccg tcacggtggc aacactgttt cacgtgaaac gttgttttcc ggcagcctcg
     4621 ggagcgatcg gaagaggttt gagcgatgca agccaaatgg cgaggtgaag cggttgccga
     4681 cagcgatgcg gtgcgcacgg tcggcggcta cgtttatttt ccgcgagccg cggtacgcat
     4741 ggacctgctc cagccttcgc caaagacggc cagcgacctt gagtgtccgc acggggtgca
     4801 attctacaat ctcgtccggc agggtgccac gagcgaacgc gccgcctggt cctacgaggc
     4861 cccccaggcc gcgctgagct cggttgcgca ctggatcggc ttctggaagg acgtcgagct
     4921 cggttgagtg cggaaggagc ccccggcctc cccgcgcttc cggcgcagct catttgccaa
     4981 tgcaaaagcg gccaaagacc tgttccaaaa cgtcctcaac ggcaatggcg ccgaccaagc
     5041 gtcccaaggc ctgcgcggcg cgccgcaggt cctcggctct gagctcagca tcctcgctcg
     5101 gtccgctcaa aaaggagctg agcgctgccc ggcagcgcaa cagctgctgg cgatgcctgg
     5161 cttgggtcag ggcgggggct tcctgatcgc cggcccgggc cgcggcgatc gcggcgagcc
     5221 gctcgattag tgtctcgagc ccatagccct tgagagccga aatggtgagg gcctccgccg
     5281 tgggcgccgg cagcgcccgg ccgtagcgca ggtcagcctt gttgtgaacg atcagcaagc
     5341 gatcggcgcg aacgcccagg tccgccgcgg gcggcggcgc cgtgggatcc gtcacgtcct
     5401 gcaaccagag caccagatcg gcggcgcgca cacgctctag ggcccgcctg atcccttctt
     5461 cctccacctt gccggcggcg gcacgaatgc ccgccgtgtc gctgacgatg accgccaagc
     5521 ctgcgagctc caatttcacc tcgagcacat cgcgcgtggt gccaggctct tcggagacga
     5581 tggcgacgtc gcgccgtgcc agcgcgttga gaaggctcga cttgccgacg ttcggcgcgc
     5641 ccgccagaac gacttgaaac ccatcgcgca ccagctcgcc gcgatggtcg tcatcgagat
     5701 gcgcggcgat ggcttcggca atgggcgcga tctggcgccg cgcctcgtcg aaagcattgc
     5761 tcgccacatc cggttcatcg gaaaaatcga tgccggcttc ggtgagcgcc atggcgccga
     5821 gcaatccctg ccgccaggcc tcatagaggc gatagagcgc gccctccgct tggcgcagcg
     5881 cttggcgacg ctgcgcggcg gtctccgcgt cgatcagatc ggcgagaccc tccacacccg
     5941 tcaaatcgag cttgccgttc tcgaaggcgc ggcgggcaaa ctctccgggc tccgccagcc
     6001 ggcagccctc gagcgaggcc agcgccgcga tgacgccttg caccaccgcg cgcccgccat
     6061 gcacctgcag ctccgccatg tcctcgccgg tctcgctggc cggaccggga aaccacaaca
     6121 ccagggcgtg atccaagacc tcggccgtgt ccggatgttt gagccgtcga aaggccgcat
     6181 agcgcggctt gggcc
//
LOCUS       DASTMT010000479         5251 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_2897472, whole genome shotgun sequence.
ACCESSION   DASTMT010000479 DASTMT010000000
VERSION     DASTMT010000479.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 5251)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5251)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5251
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2897472"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            247..2265
                     /locus_tag="VFR00_11000"
     CDS             247..2265
                     /locus_tag="VFR00_11000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011088023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl/propionyl/methylcrotonyl-CoA carboxylase
                     subunit alpha"
                     /protein_id="HEU0159836.1"
                     /translation="MFRKILIANRGEIACRIIKTARRLGIETVAVYSDADRDGLHVRQ
                     ADEAVNIGPAPAAQSYLVIEKIVEACRKTGSQAVHPGYGFLSEREAFAVALAQAGIVF
                     IGPNPKAIAAMGDKIESKKAAARAKVSTVPGHLGEIADAKQALRIAAEIGYPVMIKAS
                     AGGGGKGMRVAYSPKETEEGFALARSEAKSSFGDDRVFLEKFIENPRHVEIQVLGDKH
                     GNVIHLGERECSIQRRNQKVVEEAPSPLLDVKTRKAMGEQAVALAKAVGYDSAGTVEF
                     VAGQDRSFYFLEMNTRLQVEHPVTELVTGIDLVEQMIRIAAGEKLPWRQADVKMTGWA
                     VESRIYAEDPFRNFLPSIGRLSRYRPPPEGKTASGTIRNDTGVYEGAEISMFYDPMIA
                     KLVSHAPSREEAIALQAEALDAFVIDGVEHNIPFLATLMQHPRWKSGRLSTGFIAEEF
                     KGGFGARALAGEELEVLAAVAVAIDHLGNARRREITDQMGGPSVRFARRRIVRIGSER
                     VAAEVEEGLAGVTKVSMLDANGAARKTMELKSEWWPGEPVWQGTVAGHRVSVQVRGIL
                     NGYDLAYRGVRAKAYVYTEREAALADLMPLKLAPDTSKLLICPMPGLVKAIHVTPGQE
                     VKPGDALCIVEAMKMENVLRAEREGVVKSVQAKPGDSLAVDAVIMEFA"
     gene            2357..3943
                     /locus_tag="VFR00_11005"
     CDS             2357..3943
                     /locus_tag="VFR00_11005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006206633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase family protein"
                     /protein_id="HEU0159837.1"
                     /translation="MRSFHFPGRSAVYARHAMCATSHPSATVTALDVLRQGGNAVDAA
                     IATAAVLAVVECPMTGIGGDCFALVAKPGAKAPLALNAAGRAPAAAHAAWYAREGIQR
                     IETTSVHAVTVPGAVDGWCRLCEDHGSMPLARLLAPAIELAEQGFVVAPRVSVDWARA
                     AAKLGRHPAAAKHFLPAGRAPRVGEVARFPALAATLKRIAQEGRAGFYAGEAAQDMVR
                     TLQDLGGLHTLEDFASQSASYVEPISTRYHDIDLYELPPSNHGVVALILLKMLQRLGR
                     VGASVSVERYHVLMEAARLAFAMRDSFVADPDAAHVPLGHLMDDRVIDGLVERIDRRR
                     RRPELGPIPRPSGSDTVCFSIVDAQGMAVSFINSLFADFGSGIAADGTGVVFHNRGEG
                     FVLDPSHPNGIAPRKRPLHTLIPAMGFKAGHLALTFGVMGAHFQPMGHVYVMSNLFDY
                     GMDLQEAVDCPRVFFEGDGLLVEDAVPAPVVAGLEALGHKVKRRELPWGGAQIIARDA
                     ERGVLIGASDPRKDGMALGY"
     gene            3960..4256
                     /locus_tag="VFR00_11010"
     CDS             3960..4256
                     /locus_tag="VFR00_11010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010682556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEU0159838.1"
                     /translation="MSDATSRRRQVRVRIEGRVQGVGFRYWTERVATNLGLDGWVRNR
                     RDGGVEAVFAGSPDRVAEMLDRCRQGPRSARVDFVKVLDEPDPPPPGFEVRATV"
     gene            complement(4263..5108)
                     /locus_tag="VFR00_11015"
     CDS             complement(4263..5108)
                     /locus_tag="VFR00_11015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEU0159839.1"
                     /translation="MDIALARTFLMVAETGSFIDAARKMNVTQSTVSARIKVLEEQFS
                     RSLFDRSRNGATLTAAGEQFQKHALALVRLWQHAQLEVGLSGQHRDHLAVGAQATLWD
                     GFLLKWISWLRDNIPDIAISASASLSAVMIQRLLEGTLDLAIMYRPGQPPGLTIEHLF
                     DEEFVLVTSCSGRRRHSSDYVLMDWGPEFHAEHAAAYPDLTNTSVHLDLGSLGLDFLL
                     ANEASGYFPRRLVRQHLSRRRLRIPARARAFSCPVYMVYPETRDEEAYEPILKGLRRA
                     AQRLI"
BASE COUNT          886 a         1629 c         1810 g          926 t
ORIGIN      
        1 gcgcgacatt gccgagcggg cagcgatcgc ggcggcctga ggcgagcccc gaggggctgc
       61 agcggcgcca cggcgccagc aatctcgcca gcagaagggg caacagcccc ctctgcccct
      121 gtgcgcggcc tggcgcgctg agcaggctgc ggctcggcat cccctatcgt gcggcggccc
      181 tttgccccta tcatcggcgc ttagcgcgag tgccgtttgc gacgccgccg gggatggtta
      241 gggttcatgt tccgcaagat actgattgct aatcggggcg agatcgcttg ccgcatcatc
      301 aagacggcgc gccggctcgg tattgaaacc gttgcggtct attcggatgc cgaccgggat
      361 ggcttgcacg tgcgccaagc cgatgaggcg gtcaacattg gtccggcgcc cgcggcccag
      421 tcctacctgg tgatcgagaa gatcgtggaa gcttgccgga agacgggctc gcaagccgtg
      481 catcccggct acggcttcct gtccgaacgc gaggcctttg ccgtggccct ggcacaagcg
      541 ggcattgttt tcattggtcc gaaccccaag gcgattgccg ccatgggcga caagatcgag
      601 agcaagaagg cggccgcccg cgccaaggtg tcgaccgtgc cggggcatct gggcgagatt
      661 gccgatgcca agcaggcgct gcgcatcgcc gccgagatcg gctatcccgt catgatcaag
      721 gcgtcggcgg gcgggggcgg caagggtatg cgcgttgcct attcgcctaa ggagaccgag
      781 gaaggcttcg cgctggcgcg ctcggaggcc aagtccagtt ttggcgatga tcgtgtgttt
      841 ctcgaaaaat tcatcgaaaa cccgcggcac gtcgagatcc aggtcttggg tgacaagcac
      901 ggcaatgtca tccatctggg cgaacgcgag tgctccattc agcggcgcaa ccagaaggtc
      961 gtggaggagg cgccctcacc gctgctcgac gtcaagacgc gcaaggcgat gggcgagcag
     1021 gcggtggcgc tcgccaaggc ggtcggctac gacagcgccg gcacggtgga gtttgtggcc
     1081 ggccaggacc gctccttcta tttcctggag atgaatacgc gcctgcaggt cgagcacccc
     1141 gtaaccgagc tggtaacggg tatcgatctc gtcgagcaga tgatccgcat cgccgccggc
     1201 gaaaagctcc cctggcggca ggccgacgtg aagatgacgg gctgggcggt ggagtcgcgc
     1261 atctatgccg aggacccgtt ccgcaatttc ctaccctcga tcggacgcct gagccgctac
     1321 cggccgccgc cggagggcaa gacggcgagc ggcacgatcc gcaacgacac cggcgtctac
     1381 gaaggcgccg agatctccat gttctatgac ccgatgatcg ccaagctcgt cagtcatgcc
     1441 cccagccgtg aggaggccat tgcgctgcag gcggaggcgc tcgatgcctt tgtgatcgac
     1501 ggcgtcgagc acaacatccc cttcctcgcc acgttgatgc agcacccgcg ctggaagagc
     1561 ggccggctgt ccacgggctt catcgccgag gagttcaagg gcggcttcgg ggcgcgcgcg
     1621 ctggccggcg aggaactcga ggtgctggcc gcggtggcgg tggcgatcga tcaccttggt
     1681 aatgctcgcc ggcgggagat caccgaccag atggggggcc cgagcgtgcg tttcgcccgc
     1741 cggcgcatcg tccgcatcgg cagcgagcgg gtcgcggccg aggttgaaga aggcttggcg
     1801 ggcgtgacca aggtcagcat gctcgatgcc aatggtgccg cgcgcaaaac catggagctg
     1861 aagtccgagt ggtggccggg cgagccggtt tggcagggca ccgtggcggg ccatcgggtc
     1921 tccgtgcagg tgcgcggtat tttgaacggt tacgacctcg cttaccgtgg cgtgcgggcc
     1981 aaggcctacg tctataccga gcgggaggct gcccttgccg atctgatgcc cctgaagctt
     2041 gccccggaca cctccaagct gttgatctgt cccatgccgg ggttggttaa ggccatccac
     2101 gtgacgcccg gtcaagaagt gaagcctggc gatgccctgt gtatcgttga ggcgatgaag
     2161 atggaaaacg tcttgcgcgc ggagcgcgaa ggggtggtga aatccgtgca ggccaagccg
     2221 ggagatagcc ttgccgtcga tgcggtgatc atggaatttg cttgaacgca aggcagccgc
     2281 cagcggattt gcgggggcct cggttttcat cgcctgtcgc gcgcgatcag gccaccggct
     2341 gaaggacgtt gagttcatgc gcagttttca ctttcccggg cgctcggcgg tctacgcgcg
     2401 tcacgccatg tgcgccacct cgcatcccag tgccaccgtc accgcgctcg acgtcctgcg
     2461 ccagggcggc aatgcggtcg atgccgcaat tgccaccgcg gccgtgctgg ccgtggtgga
     2521 atgcccgatg accggtatcg gcggcgactg ctttgcgctg gtcgccaagc cgggcgcgaa
     2581 ggccccgctt gcgctcaatg ccgcagggcg cgcccccgcc gccgcgcatg cggcttggta
     2641 cgcgcgggag ggcatccaac gcatcgaaac caccagcgtg cacgccgtca ccgtccccgg
     2701 ggcggtcgat ggctggtgcc gcttgtgcga agatcacggc agcatgccgc tggcacggct
     2761 gctggctcct gccatcgagc tcgccgaaca aggcttcgtg gttgcgccgc gggtgtccgt
     2821 cgactgggcg cgcgccgccg caaagctcgg ccgtcatccc gctgccgcca agcacttcct
     2881 gccggcgggg cgcgcgccgc gggttggtga ggtggcgcgc tttcccgcgc tagcggcaac
     2941 gctcaagcgc attgcccagg aggggcgtgc cggcttctat gccggcgagg cggcccagga
     3001 catggtgcgg accctccagg accttggcgg tctgcatacg cttgaggatt ttgccagcca
     3061 atccgcaagc tacgtcgaac cgatctcgac ccgttaccac gacatcgatc tctatgagct
     3121 gcctcccagc aaccatggcg tcgtggcgct gatcctgctc aagatgctgc agcgcttggg
     3181 gcgggtgggg gcgagcgttt cggtggagcg ctatcacgtg ctgatggagg ccgcccgcct
     3241 agcctttgcc atgcgcgata gctttgtggc cgaccccgat gccgcgcacg tacccctggg
     3301 ccacctgatg gacgaccgcg tgatcgatgg cctggtggag cgcatcgacc ggcgccggcg
     3361 ccgtcctgag cttggcccga taccgcggcc gtcggggtcc gatacggtct gcttttccat
     3421 cgtcgatgcg caggggatgg cggtatcctt cattaactcg ctgttcgccg acttcggctc
     3481 cggcattgcc gccgatggga cgggggtggt tttccacaac cgcggcgagg gcttcgtgct
     3541 cgatccgagc caccccaacg gcatcgcccc gcgcaagcgc ccgctgcata cgctgattcc
     3601 cgccatgggc ttcaaggcag ggcacctggc gctcactttc ggggtgatgg gcgcccactt
     3661 ccagcccatg ggccatgtct acgtcatgag caacctgttc gactacggca tggacctgca
     3721 ggaggccgtc gactgcccgc gcgtgttctt cgagggcgat ggcctactgg tcgaagatgc
     3781 cgtgccggcg ccggtggtgg ctggattgga agcgctcggg cacaaggtca agcggcgcga
     3841 gctaccctgg gggggtgcgc agatcattgc tcgcgacgct gagcggggcg ttctgatcgg
     3901 cgcctccgac ccccgcaagg atgggatggc actcggttat tagcaaagac gcccttcaga
     3961 tgtccgatgc gacgagtagg agacgtcaag tgcgggtgcg catcgaagga cgcgtgcagg
     4021 gtgtcggctt ccgctactgg acggagcggg tcgccaccaa ccttggcctc gatggctggg
     4081 tgcgcaatcg acgcgacggc ggcgtcgagg cggtctttgc aggaagcccg gacagggttg
     4141 ccgagatgct cgaccgctgc cggcaaggcc cgcgttcggc ccgcgtcgat tttgtcaaag
     4201 tgctggacga gccggacccg ccgccgccgg gcttcgaggt gcgtgccacg gtgtgagagg
     4261 ggttagatca ggcgttgcgc ggcgcggcgc aggcctttca ggatcggctc gtaggcctct
     4321 tcgtcgcggg tctcgggata gaccatataa acggggcagc tgaaggctcg tgcgcgcgcg
     4381 gggatgcgca gccggcgtcg gctcaggtgc tggcgcacaa ggcggcgcgg gaagtaaccg
     4441 gaagcctcat ttgccaagag aaaatcaagg ccgagggagc ccaagtcgag gtgcacgctg
     4501 gtgttggtga ggtccgggta ggccgccgcg tgctcggcgt gaaactccgg cccccaatcc
     4561 atcagcacat agtcgctgga atggcggcgg cggccggaac agctggtcac cagcacgaac
     4621 tcttcatcaa acaggtgctc gatggtgagc cccggcggct gtcccggccg gtacatgatg
     4681 gcaaggtcga gcgtgccttc gagcaggcgc tggatcatca ccgccgataa cgaggcgctg
     4741 gccgagatgg cgatatcggg aatgttgtcg cgcagccaag atatccactt gaggagaaag
     4801 ccgtcccata gtgtggcttg cgcccccacg gccaggtggt cgcgatgctg cccggagagg
     4861 ccgacctcca gctgggcatg ctgccaaagg cgtaccagag ccagggcgtg cttctgaaac
     4921 tgctcgcccg ctgcggttaa cgttgccccg ttacgtgagc ggtcgaacag cgagcgactg
     4981 aactgctctt cgaggacctt gatgcgggcg ctgaccgtcg actgcgtaac gttcatcttg
     5041 cgggcagcgt cgatgaagct accggtctcc gccaccatca ggaaagtgcg tgcaagtgcg
     5101 atgtccatcg gctatgcaat cactgctaca tggatgctga aatgtatcta tcgaaatcat
     5161 cgatagtcgt cagcggaaaa atccgtttgt agaatgtcat gaaaatttcc acattgacga
     5221 cgcccggact cgattcgggt gattcactgg a
//
LOCUS       DASTMT010000480         4013 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_5716290, whole genome shotgun sequence.
ACCESSION   DASTMT010000480 DASTMT010000000
VERSION     DASTMT010000480.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4013
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5716290"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..654
                     /locus_tag="VFR00_11020"
     CDS             <1..654
                     /locus_tag="VFR00_11020"
                     /inference="COORDINATES: protein motif:HMM:NF045201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033922 - peptidoglycan
                     beta-N-acetylmuramidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exo-beta-N-acetylmuramidase NamZ
                     domain-containing protein"
                     /protein_id="HEU0159840.1"
                     /translation="ELARMIKGEAWLEGLAPLQLEVVKIENWQRGMRWPETQLSWVAT
                     SPNISTFESALLYPGIGFVGEMNVNEGRGTASPFTLFGAPWITTPRLLARLAAQPMVG
                     IRYEAAQYIPKSIEGVAQHPRFLGQEVNGVRLIVTDAARVEPLEIGMHVLAALAAEAR
                     AAGQRRLIANVAMFHALAGSKRLHRLLLAGDDGSAIVAAWRGEVEAFRARRAKYLLY"
     gene            complement(774..1664)
                     /locus_tag="VFR00_11025"
     CDS             complement(774..1664)
                     /locus_tag="VFR00_11025"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015665186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU0159841.1"
                     /translation="MTVAQAGFAERPRAVVVTGASTGIGRATARALGARGFRVFGSVR
                     RQADGEQLRADLGPLFTPIHCDVTDAVAVAAAASQVGAELKGEALAGLVNNAGVAVSG
                     PLLEVPIAELRRQLEVNLVGQMLVTQCFAPLLGAATSRVTSKGRIVMVTSTAGRRAFP
                     FMGPYAASKFGLEGLAESLRRELMLFGIDVIIVAPGPTATPIWDKAEAVDPATFGTSP
                     FAPALGKFRELMLSNGRKGLPPERIARVIVEAITAKRPKVRYTVTPTPLQNFLLSRLP
                     KRLVDRVLARTLGLTAPTTP"
     gene            complement(1852..>4013)
                     /locus_tag="VFR00_11030"
     CDS             complement(1852..>4013)
                     /locus_tag="VFR00_11030"
                     /inference="COORDINATES: protein motif:HMM:NF024986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HEU0159842.1"
                     /translation="KLKEGARAWNDWRRANPLLEPQLGELNLSVGYKQFGEAQGGPID
                     LSQADLRRAALEHATLIEADLTGAYLVQANLSHARLKGADLSGANLSDARLDHADLSD
                     AELTAATLTGANLKDARNLTQAQIEQTYGDESTLLPPGLAMPARWRKDKIKPSTELAP
                     RLAFEPVAGKVDPHILLGVSPQASLKDIRAAYVRLVKALHPDARALDPKAAEHLKAVN
                     KAYQDLKLRALNHAPVPVPVRTSFRGGAFVVGFLGSSLSLLALAGGLKYLGMFESGEL
                     PPSQQAETRAQGQRGSESSATRSAVSEDAKARGNSPEHAAEADAAARTAARAATDDAS
                     WLQAEREATSASLHRYLGRYANGRHAAEATAALPAIVNTEIALGQTLDVSAGMASARA
                     TLQHYLAAYPHGRLAPEVERKLTAIAAADAARLADRAAWAEAERTGTSDALRRYITAH
                     PTGTNAAYARQAIVALEAFQAQRTADHADWAKARADGGKTLLRGYLAAHPDGDYVADA
                     RLGLAAIEAREMLEQADHAQWAKADARGSKQALEDYLAAYPNGLHASHARDRLQALAA
                     ALPRPTVERLARVPRQAGEVIVEPQYREAAGARGQDPLSAPMPLAAPEVGLVAPDDER
                     LRDDGDWLKAQRRNTKAAYAAYLLTHPNGRHLAEARASVSGLPVAPSKFKPQAAGKGF
                     KRITQAFGAGAAEAPASQKWQSADEPFIGPDGRIRQR"
BASE COUNT          593 a         1319 c         1428 g          673 t
ORIGIN      
        1 gagctggcgc gcatgatcaa gggcgaggct tggctcgaag gccttgcgcc tcttcagctc
       61 gaggtggtca aaatagaaaa ctggcagcgc ggcatgcgct ggcccgaaac acaattgagt
      121 tgggttgcca ccagtcccaa catttcgacc ttcgaatcgg cgctgctcta tcctggcatc
      181 ggcttcgttg gggagatgaa cgtcaacgag ggccgcggca ccgccagccc gttcacgcta
      241 ttcggtgcac cgtggatcac gacgccgcgt ctgctggcgc gcctcgccgc ccagcccatg
      301 gtagggatcc gctacgaggc cgcgcagtac atcccaaaat ccatcgaagg cgtggcccag
      361 catccgcggt ttctcggcca agaggtcaac ggggtgcgtt tgatcgtcac cgatgccgcg
      421 cgcgtcgagc ccctcgagat cggcatgcac gtcttggccg cgctcgcggc cgaggcgcgt
      481 gcggcggggc agcggcggtt gatcgccaac gttgccatgt tccacgcgct ggcgggctcg
      541 aagcgtctgc atcgcctctt gctcgccgga gacgacggca gtgccatcgt tgccgcctgg
      601 cgcggcgagg tcgaagcctt ccgggcgcgc cgggccaaat atctcctcta ttgaacccgg
      661 gtgaggtgcg gtgcagcggt tggcgtggat gctgtagggc gccgggagca agcgccgcgg
      721 gcgcatagtg gagcggttcg cccccgccca ggccaagcgg aagcgcccgt tgtctatggc
      781 gtcgtcggtg ctgtcaggcc cagcgtgcgg gcgagcacac gatcgaccag gcgcttcggc
      841 agccggctca gcaggaagtt ttgcagcggc gtcggtgtaa ccgtgtagcg gaccttggga
      901 cgcttggccg taatggcctc gacgatgacg cgggcgatgc gctcgggtgg caagcccttg
      961 cggccgttgc tgagcatcag ctcgcggaat ttgcccagcg ccggcgcgaa gggcgaggtg
     1021 ccaaaggtgg cgggatcgac cgcttcggcc ttgtcccaaa tgggcgtggc cgtcggtccg
     1081 ggggcgacaa tgatgacgtc gatgccaaac agcatcagct cgcggcgcag gctttcggcg
     1141 aggccttcca aaccgaactt cgaggccgcg tagggaccca tgaagggaaa ggcgcggcgg
     1201 ccggcggtcg acgtcaccat gacgatgcga cccttgctcg taacgcgaga ggttgccgcg
     1261 cccagcagcg gcgcgaagca ttgcgtgacc agcatctggc cgacgagatt gacctcgagc
     1321 tggcggcgca attcggcgat cggcacctcg agcaacgggc cggagacggc gacccccgca
     1381 ttattgacga ggccggcgag cgcctcgccc ttgagctcgg cgcccacctg cgatgcggcg
     1441 gcggcgactg ccaccgcgtc cgtgacatcg cagtgaatgg gtgtgaagag gggacccaaa
     1501 tctgcgcgca attgctcgcc gtcggcctgc cggcgcacgc tgccgaagac gcgaaagccg
     1561 cgggcgccga gcgcgcgcgc cgtggcgcgg ccgatgcccg tggaggctcc ggtcaccacg
     1621 acggcgcgcg ggcgctcggc aaaaccagct tgtgcaacgg tcatcggccc ctcgcataac
     1681 ggtttggatg ccccaagcgg tcgacgcagc gccggccgcg gccgacaagc tagcaggtcc
     1741 cagcgcgctc gggctcaccg ttgcggcgct ttccgcaacg gcgggggcgc cgccccgtgc
     1801 cgatcgatcg gcttctcgga tcctctacgc gccgggccgc cgcctctccg cctagcgctg
     1861 acgaatgcgg ccgtcggggc caatgaaggg ttcatcggcg gattgccact tttgactggc
     1921 cggcgcctcg gccgcacctg cgccaaaggc ttgcgtaatg cgcttgaagc ctttgccggc
     1981 ggcctgtggc ttgaacttgc tcggcgctac cggcaggccg ctcacactgg cgcgggcctc
     2041 cgcgagatgc cggccgttcg gatgggtcag cagataggcg gcgtaggcgg ccttggtgtt
     2101 gcggcgttgg gccttgagcc aatcgccatc atcacgcaag cgctcgtcat ccggggcgac
     2161 gaggcctacc tcgggcgcag ccagcggcat cggcgcgctg agcggatctt ggcctcgtgc
     2221 gccggccgcc tcccgatact ggggctccac aatgacctcg cccgcctggc gtggtacgcg
     2281 ggcaagacgc tcgaccgtcg gccgcggaag ggccgccgcc agggcctgca agcggtcgcg
     2341 cgcatgggag gcgtgcagcc cgttcggata ggcggcaaga tagtcctcga gggcctgttt
     2401 gcttcccctg gcatccgcct tggcccactg cgcatgatcg gcctgttcca gcatctcgcg
     2461 tgcttcgatc gccgcaagcc ccaggcgcgc gtcggccacg taatcgccgt cagggtgggc
     2521 cgcgagataa ccacgcagga gggttttgcc gccatcggca cgcgctttcg cccagtcggc
     2581 atgatccgct gtccgctgcg cctgaaaagc ctcgagcgcg acaatggcct gacgggcgta
     2641 tgcggcattg gtgcccgtgg ggtgggcggt gatatagcgg cgtagcgcat ccgacgtgcc
     2701 cgttcgctcc gcctccgccc acgccgcgcg gtcggcaagc cgcgcggcat cggcagcggc
     2761 gatcgcggtc agcttgcgct cgacctcggg tgcgaggcgc ccgtgcggat aggcggcaag
     2821 atagtgctgc agggtcgcgc gcgcggaggc catgccagcg gaaacatcga gcgtttggcc
     2881 cagcgcgatt tcggtgttga cgatcgccgg cagggcggcc gttgcctctg cggcgtggcg
     2941 gccattggcg tagcgaccga gatagcggtg cagcgaagcg cttgttgcct cgcgctcggc
     3001 ttgcagccag gacgcatcgt cggtggcagc gcgcgccgcc gtgcgtgcgg ccgcgtcggc
     3061 ctcggccgcg tgctcggggc tgttgccgcg cgccttggcg tcctcggaca ccgccgagcg
     3121 tgtcgccgaa ctctcgctgc cgcgttggcc ttgcgctcgg gtttctgctt gctggctcgg
     3181 gggcagctcg ccgctctcga acatgcccag atatttgagg ccgccggcca gcgccagcag
     3241 gctcaggctg gagccgagaa agccgacgac gaacgcgcca ccgcggaacg acgtccgcac
     3301 cgggactggc acgggagcgt ggttgagcgc gcggagcttc agatcttgat aggctttgtt
     3361 cacggccttc aagtgctcgg cggccttggg atcaagggcg cgcgcgtccg gatgcagcgc
     3421 cttgaccaac cgcacatagg cggcgcggat gtccttgagg ctggcctgcg ggctgacgcc
     3481 gaggagaatg tgcgggtcga ccttgcccgc gacgggttcg aaggccaagc gcggcgcgag
     3541 ctccgtcgac ggctttatct tgtccttgcg ccagcgtgcc ggcatggcga gacccggcgg
     3601 cagcagcgtg ctctcatcgc cataggtttg ctcgatttgc gcctgcgtca ggttgcgggc
     3661 gtctttgagg ttggccccgg tcagggttgc cgccgtgagc tcggcgtcgc tgaggtcggc
     3721 atgatcgagg cgcgcatcgc tgaggttggc gccgctcaga tcggcgccct tgagacgcgc
     3781 atgcgagagg ttggcctgca ccaagtaggc acccgtcaga tcggcctcga tcaacgtcgc
     3841 gtgctcgagc gctgcgcgac gcagatccgc ttggctgagg tcgatggggc ctccctgcgc
     3901 ttcgccgaac tgtttgtagc cgacggaaag attgagctcg ccgagttgcg gttcgagcaa
     3961 tggattggcg cgccgccagt cattccaagc gcgcgcaccc tccttcagtt tac
//
LOCUS       DASTMT010000481         4571 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_3555336, whole genome shotgun sequence.
ACCESSION   DASTMT010000481 DASTMT010000000
VERSION     DASTMT010000481.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4571)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4571)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4571
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3555336"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..412)
                     /locus_tag="VFR00_11035"
     CDS             complement(<1..412)
                     /locus_tag="VFR00_11035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007601506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HEU0159843.1"
                     /translation="MRTKVTAIETLACDAGWRNYHFVKLSTSDGVVGWSEFDEGFGSP
                     GVGAIVERLAPRVVGQLVGEHERIYQELYSLTRPGSGGVVAQALGAIENALLDAKART
                     LAVPCYELLGGKIRDRIRVYWSHCATWRINHPTFY"
     gene            complement(662..1456)
                     /locus_tag="VFR00_11040"
     CDS             complement(662..1456)
                     /locus_tag="VFR00_11040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HEU0159844.1"
                     /translation="MAFHYAGCASQGARSYQEDAAALRTAAGEDVPIGQGQAGSATEF
                     TLALADGMGGHAGGALASALACRTYLAAMASSSGNLSARLDEALTLANGAIADYVAEH
                     PALNGMGCTLVGAAFGAFGVEWVSVGDSPLYLVRLGKIELLNEDHSLAPELDKLAAAG
                     KISRAEARHDPRRHFLRSALTGSEPELVDRPHRPLRLEPNDVIVLASDGIGSLEKDEI
                     LATVEAHAAAGPAGIANALLRAVDGKGEIYQDNTTIVVVVVGAAAA"
     gene            complement(1459..2286)
                     /locus_tag="VFR00_11045"
     CDS             complement(1459..2286)
                     /locus_tag="VFR00_11045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEU0159845.1"
                     /translation="MKDMKRGALETPASTAGASIPNRILGSLRDALNAASRESTGEVS
                     GSRNAEEPSARPAALAAGASAPAELRPANAPARLPEAPPLPTQPVSAAEALRLAKTPA
                     PELVSVEDKEPTTRVVRAGPALKPAPATAAPEEVGRTQAVRGKPKVTRTTFHQDPVVG
                     WMVVVGGPGLGAYRPIYEGNNTLGRGKGQRIPIDFGDESISAEEQAYIRYDSVDRSFL
                     FVPNLAKTNIVSINNKKPTGAVPLNAMDVITVGRTQLAFVPFCGEEFDWSELTDLKE"
     gene            2727..>4571
                     /locus_tag="VFR00_11050"
     CDS             2727..>4571
                     /locus_tag="VFR00_11050"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEU0159846.1"
                     /translation="MQPKPASGTNLLALPEGTELVGDYRIKRVLGAGGFGITYLAEEI
                     ALGRLVTIKEYFPADFAARVGTINAAPRSQGSAEDFKWGLDRFISEAKTLARFVHPNI
                     VGVYRYFLANDTGYMVIAFEEGASFKSWLARLKRAPRQAELDRILTPLLEALEFVHKA
                     NFLHRDIAPDNIIIRTDGSPVLIDFGSARGEMISHSRTVSALVKPGYSPYEQYATTAS
                     KQGPWTDIYALGATLYHAITGKRPPDSPSRMVADEYIAAREAALSSYRASFLAAIDQA
                     LVLEVTERPQSIAAWRGTLLSPDPKRASAGLGLRLALVRTLGRRRSEALPAPAAAGKA
                     ALTVRAPDQEKTLVPLPPDAPQPKGQLLDFIDALKSRRAPRKPAPKAEAPPAAPLAAR
                     RRSAKKRAAGSEAEPPSSAKRLFGFGRVPYAPATPARAPKPAAAKKEPPAAKLADDRA
                     PAARARRGRRWRLPSRRWRSLIYKLAAGLMIAWLAVSYQSRPPPEEQRGAVMPATALA
                     QLGRLSGHTGPVTALASDDARHWIVSSGADASLKIWHSLSGALLRSIDLEEGPATTLA
                     LDDRRALTGHRSGAIVLWDLQRAERLQVYHLQDGPISALAFTDDPNHFA"
BASE COUNT          726 a         1571 c         1506 g          768 t
ORIGIN      
        1 ggtaaaaggt cggatggttg atgcgccagg tggcacagtg cgaccagtac acgcgaatac
       61 ggtcgcgtat cttgcccccc aacaattcgt agcagggcac cgcgagcgtg cgggccttgg
      121 catccaatag ggcgttctcg atggcgccga gcgcctgggc caccacgccg ccggagccgg
      181 gtcgcgtcag gctatagagc tcttggtaga tgcgctcgtg ctcgcccacc aactggccga
      241 ccacccgcgg cgccaggcgc tcgacaatcg ccccgacccc cggcgagcca aagccttcat
      301 cgaattcgct ccagcccacc acgccatcgc tggtgctgag tttgacgaag tggtagttgc
      361 gccaccccgc atcgcaggcc agcgtttcga tcgccgtcac tttcgtgcgc attttgtgct
      421 ccgccggtgc ccccagactg ccgaagaaaa tcgcgcgtgc gcacccggca gcgcgcgacc
      481 ggcacggtcc tggcggcgta ggcaagactg cgcacgcagc tgctgcgacc gccgaccggt
      541 ccccgccgct ctggggccca aagcaaagag cggcatctgt cgagggcacg gtagcgccga
      601 agggggtggc ttgtcaccgt agccgccctc ccttcgcccg cccggccccg cgccctgcgc
      661 ctcaggccgc ggccgcaccg accaccacca ccacgatggt ggtattgtcc tgatagattt
      721 cgcccttgcc atcgaccgcg cgcaggaggg cattggcgat gcccgcagga cctgccgcgg
      781 cgtgcgcctc cacggtagct aaaatctcat ctttctcgag gctgccgatg ccgtcgctgg
      841 caagcacgat cacatcgttg ggctcgaggc gcagtgggcg atggggtcgg tcgaccaact
      901 ccggctcgct acccgtcaag gcggagcgca gaaaatgtcg gcgggggtca tggcgcgcct
      961 ccgcacgact gatcttgccc gcggcggcca gcttgtcgag ttcgggcgcc agcgaatgat
     1021 cctcattgag gagttcgatc ttgcccaggc gaacgagata gagcgggctg tcgccaacgc
     1081 tgacccattc gacgccgaag gcgccaaatg cggccccgac cagcgtacag cccatgccat
     1141 tgagcgcagg atgctccgcc acgtagtcgg cgatggcgcc gttggccagc gtcagggcct
     1201 cgtcgagacg ggccgaaagg ttgcccgagg aggaggccat ggcggcgagg taggtacggc
     1261 aggcgagcgc gctcgccagc gcgccgccgg catgtccgcc catgccgtcg gccagcgcca
     1321 gcgtgaattc ggtcgcgctg ccggcctggc cttggccaat cggcacgtcc tcgccggctg
     1381 ccgtgcgcag ggcggccgcg tcctcctggt agctgcgcgc gccctgactg gcgcaaccgg
     1441 catagtggaa cgccactgct actccttgag gtctgtcagt tccgaccagt caaactcctc
     1501 gccacagaat ggcacaaagg cgagctgcgt gcgccctacc gttatcacat ccatggcgtt
     1561 gagcggcacg gcccccgtcg gctttttatt gttgatggag acaatgttgg tcttggccag
     1621 gttgggcaca aacagaaacg agcggtcgac cgaatcgtag cggatgtagg cctgctcctc
     1681 ggccgagatg gactcatcgc caaaatcgat gggaatgcgc tgccccttgc cgcgtccgag
     1741 cgtgttattg ccttcataga ttggccgata agccccgagg cccggtcccc cgaccaccac
     1801 catccagccg accacgggat cctgatggaa ggtggtacgt gtgaccttgg gcttgccgcg
     1861 cacggcctgc gttctgccga cctcctccgg ggcggcagtg gcgggcgccg gcttgagtgc
     1921 cggcccggcg cgcacgacgc gggtcgtcgg ctccttgtcc tcgacgctga caagctcggg
     1981 tgcgggcgtc ttggcgaggc gcagcgcttc ggccgcgctg acgggctggg ttggcagggg
     2041 aggcgcttcg ggtaggcgcg ccggcgcgtt tgcaggtctc aattcggcgg gcgcgctggc
     2101 gccggcggcc agagcagccg ggcgcgccga gggctcctcg gcattgcggc tgccgctaac
     2161 ctccccggtc gattcccggc tggcggcatt gagcgcgtcg cgcagactgc caaggatgcg
     2221 attggggatc gaagcgccgg cggtcgacgc cggggtctcg agggcgccgc gtttcatgtc
     2281 cttcatggtc tctccctcct aggtgcgagg cggtgagcgg caccgctgcg ccgtgtccag
     2341 gtctcggcaa tgacggaacg caaatgggcg cgcgcggggt gcagcggtga gctggcaagt
     2401 gcaacaccgc cacaggcgtc gtgcaccgcc ctttctttca acagcgcacg cgatgcacgc
     2461 gacgcttgtg gctggcttcc cgcacgaatc gttcgcatcg ttgcaagagt atgacggcag
     2521 gaacatggcc gcacctctgc cctgttgcac attcgccgcg ctggcgctga gaggctcatt
     2581 gttccgccat attattgcct gtaatgctgt cgcgttcggt tcgtgagtgc gggcgggaca
     2641 ggggcgccga ttggcgccgg caagaagccc aaatctgtcg cggggttgcc ccctgcaagc
     2701 tcgtatgggc cgcaatgggg aaggcgatgc aacccaagcc ggcgtccggc acgaaccttc
     2761 tggctctgcc ggaaggcacc gagctggtcg gcgattaccg gatcaagcgc gtgctgggtg
     2821 ccggagggtt cggcatcact tacctggccg aggaaatcgc gctcggtcgc ctcgtcacca
     2881 tcaaggaata ttttcccgcg gattttgccg ctcgcgttgg caccatcaat gccgcaccgc
     2941 gatcgcaagg cagcgcggaa gacttcaagt ggggtctcga ccgctttatt tccgaggcca
     3001 agacgctggc ccgcttcgtg caccccaaca tcgtcggtgt ctatcgttat ttccttgcca
     3061 acgataccgg ctacatggtg atcgctttcg aggagggcgc cagtttcaaa agctggctcg
     3121 cgcgcttgaa gcgcgcgccg cgccaggcgg agctcgatcg tattctgacg ccattgctcg
     3181 aggcgctcga gttcgtgcac aaggccaatt tcctgcaccg cgacattgcc ccggacaaca
     3241 tcattattcg caccgatggc tcgccggtgc tgatcgattt cggttccgcg cgcggtgaga
     3301 tgatctcaca ttcgcgtacc gtgagcgcgc tggtgaagcc gggctatagc ccctacgagc
     3361 agtacgccac caccgccagc aagcaaggac cgtggaccga tatctatgcc cttggggcga
     3421 cgctctatca tgccattacc ggcaagcgcc cccccgattc gccctcgcgc atggtggccg
     3481 atgagtacat tgccgcgcgc gaggcggcgc tcagttccta ccgggcctcg tttctggccg
     3541 cgatcgacca agctcttgtg ttggaggtga cggaacgtcc gcaatcgatc gcggcgtggc
     3601 gaggcacgct gttgtcgccc gatcccaagc gcgccagcgc cggcctcggg ctacgcctgg
     3661 ccctggtgcg caccctgggc cgccggcgtt ctgaggcatt gccggcgcca gcggcggccg
     3721 gcaaggcggc cctgaccgtg cgtgcgcccg accaggaaaa aaccctggtg cccctgccgc
     3781 ccgacgcgcc gcagcctaaa ggccagctgc tcgatttcat cgatgccttg aaaagccgcc
     3841 gtgcgccgcg caagccggca ccgaaggcgg aggcgccgcc agccgcgccc cttgccgccc
     3901 ggcgtcgctc ggcgaaaaag cgcgccgcag gctccgaggc cgagccccca tcgtcggcca
     3961 aacggctgtt cggcttcggt cgcgtgccct acgcgccggc gacgccggcg cgcgcgccca
     4021 agcccgccgc ggccaagaag gagccgcccg cggcaaaact cgcggacgac cgcgcgcccg
     4081 ccgcgcgggc ccggcgtgga cggcgttggc gcctgccgtc gcggcgttgg cgctccttga
     4141 tctataagct ggccgccggg ctgatgattg cctggcttgc cgtcagctac cagagccgac
     4201 cgccgcccga ggagcaacgg ggtgcggtta tgccggccac cgcccttgcc cagctgggac
     4261 gactgagcgg acacactggc ccggtcaccg cgctggccag cgacgacgcg cgccactgga
     4321 tcgtctcctc gggcgcggac gccagcctta aaatctggca cagcctgtcc ggtgcgctcc
     4381 tgcgcagcat cgacctcgaa gagggtcccg ccaccacgct cgcgctcgac gaccgacgcg
     4441 ccctgacggg tcacaggtcg ggggccatcg tgctctggga cctgcagcgc gccgagcggc
     4501 tacaagttta tcatctgcag gacggcccga tcagcgcgct ggccttcacc gatgatccca
     4561 atcatttcgc c
//
LOCUS       DASTMT010000482         5596 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_2246329, whole genome shotgun sequence.
ACCESSION   DASTMT010000482 DASTMT010000000
VERSION     DASTMT010000482.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 5596)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5596)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5596
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2246329"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..381)
                     /locus_tag="VFR00_11055"
     CDS             complement(<1..381)
                     /locus_tag="VFR00_11055"
                     /inference="COORDINATES: protein motif:HMM:NF014816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HEU0159847.1"
                     /translation="MLKLYYAPGACSLAAHIVLEESGEKYDLYRVDLANGEQRSEAYL
                     KIHPQGRVPALQLDSGENITENTAILPYLGKRFGLWPSDPLAETRALSLIGLFAASVH
                     TAHAHISRPERYASDRAVFANLQEV"
     gene            616..2145
                     /locus_tag="VFR00_11060"
     CDS             616..2145
                     /locus_tag="VFR00_11060"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEU0159848.1"
                     /translation="MRPSHQRGFAFGLALGMIAAALALLPLALAAGPDSLGVGAGRRL
                     GPERGSDLAFAKAPALRSRAPAGQAFELCAEDPEAAAESVRRVRCAVPPGGLGPLTGP
                     ILPSVPPAPATPAPEPPAVPANANEPGKLASPPPLTDPGFRYHAPGLLAVRDATAKRG
                     RNDDRKVYAPQIVFPIRLDGGRFASMNSQIWGYGGGGYNGVGEAGGTECDPRNYDPMQ
                     QRDNFCEVRGWSMPLCPSGEGHQGQDIRPPSCKDNAWDVVAVVDGIITLVTSNTTVKL
                     KGNDGTVFEYLHMHPSSIKVKEGEKVKQGDVVGRVSKYMNGTRSTTYHLHFNVRQRIQ
                     VGNKVLEVYVPPYASLIAAYRRSKGLDAGIDAAGDLAPDPMLEIGAVAQAPTQPPAPS
                     APPSDTPPAPAPSPAPEPPAPSAPPATPPSPTPAPPTPPEPPAPPVPAPTPAPAPSPP
                     APAPAPTEPAPAPSPPTPAPTPAPTPAPPAPPPAPSEQPKSWWQRGLDKAMELWNRWW
                     K"
     gene            2214..4403
                     /locus_tag="VFR00_11065"
     CDS             2214..4403
                     /locus_tag="VFR00_11065"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEU0159849.1"
                     /translation="MRALVIAAALAAWSGTALGQSSTPEDLDRLKRMLDERLPQAPRT
                     RSLSKGERPLRSDDFYSIRRREQDGQANPSLTPGKRSALDAPGLFGVPAAAAAEGDGA
                     REPSAAAAPRGSQRVQAKRDSYVIVLKTDLDGQQLDAALATLSQKYNLEITRANKLGE
                     IRVSPRQAATRGLAPAPRTESLGAALEPQIIKELRNEPFVDAAYVDFLVSPKALPRRT
                     DTKVLAGGNTYSWSWRTGETSDGNWGLKLMRMPPVWTILNRVRKIDPDRPRTRMAFLD
                     VGFGTHGHINYNAVLGGMPANPPKAECTYSHGTHVAGIAGALFGKGRGIDGMVPDSKI
                     DAIPVSSEVWLEGTSLGLTNQLIQRAMLFSGAVDSLIDFMDQYPLKPGEKRVVNASLG
                     YNWSALGFEFGKDAEEDENVKAHIFNSARSVQRLAAKYKDSILIVAAAGNDSEGLATP
                     HWAEFASPFGFAATHESPTYQKASNILIVEALGRDGKRASFSNSGGHVSAPGVDIMST
                     LAGVSDAYGLCSGTSQATPHVTALAAILFELDPKKSPAEIAEIIRSAAVPAAERTTPR
                     VDALAAVLKLSKDNIRYLVDLNGDGKVDSADLEILKAHLLILDEARRNGTPIAIDLNG
                     DGDVNENEGCWPLVDFVGAGKALYFDAPAQAAATAARTDLEMLEDGWTDSLKTFRAAM
                     SEVGLEQLMAQWKTAPGGSALIASVAAGPRPEAAPPTTASIAASGCR"
     gene            4434..>5596
                     /locus_tag="VFR00_11070"
     CDS             4434..>5596
                     /locus_tag="VFR00_11070"
                     /inference="COORDINATES: protein motif:HMM:NF013604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0159850.1"
                     /translation="MRMSVPLAGILAAVGAAVLAASALSFPPSAAAQDVRSEVGRAVD
                     ELRKANQSLAVEVDRSTGLPSSIKGLTPRPDPSVALTATRSLSGEPSEEDVRRAVEAF
                     MATSQIRSAFPQGNAQERKVVTQVRRDPDIKGQSVAHVTQRLNGIPVFGSSAKYVVNP
                     ALAVTDITASYSTVAISGTTPSITADEAAEVARNHVRDQLSKRARDPGLDRLFASLDS
                     LQPKSELMIFDPALLRTRGANPGPLRLTWLVSVDSLRVFVDAENRNVLFFYRDHPSVM
                     VRRVYDLSSNQVFPGQKVLDEETNERVDLIPADAMRAFQNTGFVRDFFYIVLGRSGLE
                     AAGAKANMPLESYVRYSSEQNARWCKDQSVNCPKANVMVYGPGFAGALDVVGH"
BASE COUNT          972 a         1925 c         1816 g          883 t
ORIGIN      
        1 cacctcttgc agatttgcga acaccgcccg atcgctcgca taccgctccg gtcggctgat
       61 gtgagcatgt gcggtgtgca cgctggcggc gaacaggccg atcaacgaca gcgcccgtgt
      121 ttcggcgagg gggtcgcttg gccacaggcc aaaacgcttg ccgaggtagg gcaggatggc
      181 ggtgttctcc gtgatgttct cgccactgtc cagttgcaaa gctggcacgc gcccctgcgg
      241 atggattttc agataggctt cgctgcgttg ttcaccattg gccaaatcga cccgataaag
      301 gtcgtatttc tcgccgcttt cctcgagcac gatatgtgcg gcgagcgagc aggctcccgg
      361 cgcgtaatag agcttcaaca cgacaacctc cggcgcttcc cgtgatcttg aaagaacggc
      421 aaaaaagcta gcctaccacg ttgccaaatt cgagagactt gtgggcttgc ggctggcgcc
      481 gggccacgcg gcgcgcgctg ggtcaatttg ccttgctggc gcaggcgaaa aaggtatgct
      541 gcgcggcagg ggatggcgat cgagggacct ttggcctagc gcaagtcttg tacgggtgtg
      601 agggggttgg gggcagtgcg gccaagtcat cagcgtggct ttgcgtttgg cctggcgctc
      661 ggcatgatcg ccgccgccct ggcgcttctg ccgctggcgc ttgccgccgg gcccgacagc
      721 ctcggcgtcg gagcgggccg ccgcctcggc cccgagcgcg gcagcgatct cgcttttgcc
      781 aaagccccgg cactgcgctc gcgtgctccg gccgggcagg cgttcgagct ctgcgccgag
      841 gatccggaag ccgccgccga gagcgtgcgc cgcgtgcgct gcgccgtgcc gccaggcggc
      901 ctcggaccgc tgacgggtcc gatcctccca agcgtgccgc cggcgccggc aacacccgcg
      961 cccgagccac cggccgtgcc cgccaacgcc aacgagcccg gcaagctggc aagcccgccg
     1021 ccgctgacag atcccggctt tcgctatcac gcccctgggc tgttggccgt acgcgatgcc
     1081 accgccaagc gcggccgcaa cgacgaccgc aaggtctatg cgccgcagat cgtctttccc
     1141 atccggctcg acggcggccg cttcgcctcg atgaattcgc agatctgggg ttacggcggg
     1201 ggcggctata acggcgtcgg cgaggccggc gggaccgaat gcgatccgcg caactacgac
     1261 cccatgcagc agcgcgacaa cttctgcgaa gtgcgcggct ggagcatgcc gttgtgtccg
     1321 agcggggagg ggcatcaggg acaggacatt cgtccaccga gctgcaagga caacgcctgg
     1381 gacgtggtcg ccgtggtcga cggtatcatc accctggtga cctcgaatac gaccgtgaag
     1441 ctcaagggca acgacggcac ggtattcgaa taccttcaca tgcacccgag ctcgatcaag
     1501 gtgaaggagg gcgagaaggt caagcagggc gatgtggtgg ggcgcgtctc caagtacatg
     1561 aacggtacgc gcagcacgac ctaccacctg catttcaatg tgcgccagcg catccaagtc
     1621 ggcaacaaag tgctcgaggt ctacgtccca ccctacgcct ccctgattgc cgcctaccgg
     1681 cgcagcaagg gactggacgc cgggatcgac gcggccggcg atctggcccc cgatcccatg
     1741 cttgaaatcg gcgccgttgc ccaggcacca acgcagccgc ccgccccgtc ggcgccgccc
     1801 tccgataccc cgccggcccc cgcgccgagc ccggctccgg aacctccagc gccgtccgcc
     1861 ccgcccgcga cgccgccaag cccgacaccc gcgcccccga cgccgcccga gccgccggcg
     1921 ccaccggtgc cggcgcccac gcccgcaccc gccccgtcgc ctccggcccc ggctcccgcg
     1981 ccgaccgagc ccgcaccggc accgagcccg ccgacgccag cgccgacgcc ggccccaaca
     2041 cctgcgcctc cggccccgcc gccggcgccc tccgagcagc ccaagagctg gtggcagcgc
     2101 ggcctcgaca aggcgatgga gctatggaac cgctggtgga agtgagccgc gtcggcacgg
     2161 cgcaacggtg acgcccggca cggcttggag cggggaaaca ggggaggctt ggcatgcgtg
     2221 cgctcgtgat tgccgcagcg ctggctgcgt ggagcgggac ggcgctgggg caatcctcca
     2281 caccggaaga tctcgaccgc ctcaagcgca tgctggacga acgcctgccg caggcccccc
     2341 gcacgcgcag tctgagcaag ggcgagcgtc ccctacggtc ggacgatttt tactcgatcc
     2401 ggcggcgcga gcaggatggc caggccaacc ccagtctcac cccgggcaag cgctcggcct
     2461 tagacgcacc cggcctgttt ggcgtcccgg cggcggccgc ggcggaaggc gacggcgcgc
     2521 gcgagccctc ggcggcggcg gcgccgcgcg gctcgcagcg cgtccaggcc aagcgtgaca
     2581 gctatgtgat cgtgctcaaa accgacctcg acgggcagca gctcgatgcc gcgctcgcca
     2641 ccttgagcca gaagtataac ttggagatca cgcgcgccaa caagctgggc gagatccgcg
     2701 tctcgccgcg acaggcggcc acgcgcggcc tcgcaccggc gccgcgaacc gaatcgctcg
     2761 gcgcggcgct cgagccgcaa atcatcaagg agttgcgcaa cgagcccttt gtcgacgcgg
     2821 cctacgtcga ttttctggtg tcgccgaagg ccttgccaag gcgaaccgac accaaggtcc
     2881 tggccggcgg caacacctat agctggagtt ggcgtaccgg cgaaaccagc gacggcaact
     2941 gggggctgaa gctgatgcgc atgcccccgg tctggaccat cctcaatcgc gtgcgcaaga
     3001 tcgatcccga ccggccgcgc acgcgcatgg cctttctcga cgttggtttc ggcacgcacg
     3061 gtcatatcaa ctataatgcc gtgcttggcg gcatgcccgc caatccgccg aaggcggaat
     3121 gcacctatag ccatggcaca cacgtggcgg gcatcgccgg cgccttgttc ggaaaggggc
     3181 gcggcatcga cggcatggtt cccgactcca aaatcgatgc cattccggtc agcagcgagg
     3241 tgtggctgga gggaaccagt ctcggcctca cgaaccaact gatccaacgc gccatgctgt
     3301 tcagcggggc ggtcgattcg ctgatcgact ttatggatca ataccccctc aagcccggcg
     3361 agaaacgggt ggtcaatgcc agtctcggct acaactggtc ggcgctcggc ttcgagttcg
     3421 gcaaggatgc cgaggaggac gagaacgtca aggcacacat tttcaacagc gcccgctccg
     3481 tgcagcgcct ggccgccaaa tacaaggaca gcattttgat cgtcgccgcc gcgggcaacg
     3541 actccgaagg cctcgccacg ccgcactggg ccgagttcgc gagcccgttc ggcttcgccg
     3601 ccacgcacga atcgccgacc tatcagaagg cgagcaacat cttgattgtc gaggctctcg
     3661 gccgtgacgg caagcgcgcc tcgttttcca attccggcgg tcacgtctcc gcgccgggcg
     3721 tcgatatcat gagcacgttg gccggcgtca gcgacgccta cgggctatgc agcggcacct
     3781 cgcaggcaac cccgcacgtc acggcgcttg ccgccatcct gtttgaactc gacccgaaaa
     3841 aatcgccggc ggagatcgcc gagatcattc gcagcgccgc ggtacccgca gcggagcgca
     3901 cgacgccgcg ggtcgatgcc ctcgctgcgg tcctcaagct gtccaaggac aacatcaggt
     3961 acctggtcga cctcaatggt gatggcaagg tggacagcgc tgatctcgaa attctcaagg
     4021 cccatctcct gatcctcgac gaggccaggc gcaacggcac gccgattgcg attgacctca
     4081 atggtgacgg cgacgtgaac gagaacgagg gttgctggcc tctggtcgat ttcgtgggtg
     4141 ccggcaaggc tctctatttc gacgccccgg cgcaggcggc cgcgacggcc gcgcgcaccg
     4201 atctcgagat gcttgaggat ggctggaccg atagcctcaa gacgttccgg gcggccatga
     4261 gcgaagtcgg attggagcaa ttgatggcgc aatggaagac ggcgccgggt ggctcagcgc
     4321 tgatcgccag cgtcgctgcc gggccgcgcc cggaggcggc gccgccgacc acggcttcga
     4381 tcgcggcgtc gggctgtcgc tagcaagtgt gggccaaggg gagatcgcgc atcatgcgta
     4441 tgtcagtgcc attggcgggc atccttgccg ccgtcggcgc cgccgtgctg gcggccagtg
     4501 cgttgagctt tcctccaagc gccgcggcgc aagatgtgcg cagtgaggtt ggccgcgcgg
     4561 tcgacgaact gcgcaaggcc aaccagagcc tggccgtcga ggtcgaccgc tctaccggcc
     4621 tgccgagctc gatcaagggt ctgaccccgc gacccgatcc ctcggtcgcg ctcaccgcga
     4681 cgcgcagcct cagcggggag cccagcgagg aggacgtccg ccgcgcggtc gaagctttca
     4741 tggccacgag ccagatccgc tccgcctttc cgcaaggcaa cgcccaggag cgtaaggtcg
     4801 taacccaagt tcgccgcgat cccgacatca agggtcaaag cgttgcccat gtcacgcagc
     4861 gcctcaatgg cattcccgtg ttcggctctt cggccaagta tgtcgtcaat ccagcgctgg
     4921 ccgtgaccga tatcaccgcc agctactcga cggtggccat cagcggcacg acgccgagca
     4981 tcacggcgga tgaggcggcc gaagttgcgc gcaatcacgt gcgcgaccag ttgtccaagc
     5041 gcgcgcgcga tcccggattg gatcggctgt tcgccagcct cgatagcctt cagcccaagt
     5101 cggagctcat gatcttcgac ccggccctgt tgcgcacgcg tggcgccaac cccggcccgc
     5161 tgcgtcttac ctggctggtg tcggtcgact ccttgcgcgt gttcgtcgac gcggagaacc
     5221 gcaacgtctt gtttttctac cgcgaccatc cctcggttat ggtgcgtcgg gtttacgacc
     5281 tgtcgagcaa ccaggtattc cccgggcaaa aagtgctgga cgaggaaacc aacgagcgcg
     5341 tcgatctcat tccagccgat gccatgcggg ccttccagaa cactggcttc gtgcgtgact
     5401 tcttttacat tgtgctcggc cgtagcgggc tcgaggcggc cggcgccaag gccaacatgc
     5461 cgctcgaatc ctatgtgcgc tacagcagcg agcagaacgc gcgctggtgc aaggatcaaa
     5521 gcgtcaactg cccgaaggcc aacgtgatgg tgtacggtcc gggttttgcg ggcgcgctcg
     5581 acgtggtcgg ccacga
//
LOCUS       DASTMT010000483         3484 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_4406564, whole genome shotgun sequence.
ACCESSION   DASTMT010000483 DASTMT010000000
VERSION     DASTMT010000483.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3484)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3484)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3484
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4406564"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2015)
                     /locus_tag="VFR00_11075"
     CDS             complement(<1..2015)
                     /locus_tag="VFR00_11075"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009763155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HEU0159851.1"
                     /translation="MAKPARRKKAVKRLERPAAARNPLLEAWTGPFELPPFARLRSEH
                     FLPAFDRALAHNLTELAAIANQRAQPTFANTIEALERSGRTLDRVASVFFNLSGTDTS
                     PEIEEIERKIAPRLAKHSMRIYQDAKLFKRVAALTRQAASVELSEEQRRVLERYHRAF
                     VRAGAQLKAGARRRLAVIAQRLAKLATKFNQNVLADEQAFLMVLAAEDLVGLPDALRA
                     AAAETANARGLPGKYAISLARSSIEPFLQFSARRDLREKAFQGWISRGANGGQTDNRK
                     LVAEILSLRAERARLLGFDTAAAATLEFSMAKTPKAVRRLLMDVWRPARARARQERDV
                     LQKTIVAAGGNFRLAPWDWRYYAEKVRKEKFDIDEGLIKPYLQLENVIAAAFDVAQRL
                     FGIVATERRDLSLYHPDARAFEIKDAAGRQLGLFVGDYFARPSKRSGAWMSSWREQQQ
                     LDGQVRPLVVNVMNFAKGAPGEPTLLSSDDARTLFHEFGHALHGLLSNVTYPSVSGTS
                     VERDFVELPSQLYEHWLLQPEVLRRHARHYQTGKPISSALIKRLKVARNFNQGFQTVE
                     YLASAIVDLELHVLQDTKALNVDSFERQTLDSIGMPDEIVMRHRIPHFQHIIGGYAAG
                     YYSYLWSEVMDADAFEAFEATGDVFDAKMARRLYDTIYSAGGRRDPAA"
     gene            complement(2149..2877)
                     /locus_tag="VFR00_11080"
     CDS             complement(2149..2877)
                     /locus_tag="VFR00_11080"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU0159852.1"
                     /translation="MAGRVGGKAVIVTGAGHGIGASIARRLAAEDWQVLCADIDPAAA
                     QGVAATIGAAAVQCDVGSEVEIERLLALARTRFGRIDAIVSNAGIGKFAPLAQTSLED
                     WNRVLATNLTAAFLLAKSGAADLRQKRGSMLLIGSTRAHMSEPNTLAYSASKGGLVAL
                     AHALAMTLAPEVRVNVISPGWIDTGRHGTLKASDHAQHPVGRVGEPDDVAAAASYLLS
                     AAAGFVTGAELIVDGGMTRKMIYS"
     gene            complement(2945..>3484)
                     /gene="ubiG"
                     /locus_tag="VFR00_11085"
     CDS             complement(2945..>3484)
                     /gene="ubiG"
                     /locus_tag="VFR00_11085"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008689 - 3-demethylubiquinone-9
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HEU0159853.1"
                     /translation="DVGCGGGLIAEPLARLAALVTGLDPAQANIEAARQHAAAQGLAI
                     DYRAGRIEDLAGEGLSYDAVICLEVIEHVPDVGAFLATCARVLRPGGLMLLSTINRTL
                     KAYALAIIGAEYVLRWLPVGTHRWDRFVTPQELRQHLRAAGLGEPALQGLFYNPLRDE
                     WSLGSDTDVNYLAAAGKPA"
BASE COUNT          592 a         1120 c         1167 g          605 t
ORIGIN      
        1 gccgccggat cgcgccgccc gccggccgaa tagatcgtgt catacagccg gcgcgccatt
       61 tttgcatcga agacgtcacc cgtggcctcg aaggcctcaa atgcatccgc gtccatgacc
      121 tcggaccata gatagctgta atagccggcc gcatagccac cgatgatatg ttggaaatgc
      181 gggatgcggt ggcgcatgac gatctcgtcg ggcatgccga tactgtcgag ggtctgccgt
      241 tcgaagctgt cgacgttcag ggccttcgtg tcctgcagga catgcagctc gagatcgacg
      301 atggcggatg ccaggtattc gacggtctga aaaccttgat tgaaattgcg ggcgaccttg
      361 aggcgtttga tgagcgcgga cgaaatgggc ttaccggtct ggtagtgccg agcatggcgg
      421 cggagcactt ctggttgcag caaccaatgc tcgtaaagtt gcgacggcag ctccacgaaa
      481 tcgcgctcca cgctggttcc cgagacgctg ggataagtga cgttcgacaa taggccatgc
      541 aatgcgtggc cgaactcgtg gaacaaggtg cgcgcatcgt cgctgctgag cagcgtgggc
      601 tccccggggg cgcccttggc aaagttcatg acattcacca ccaacgggcg aacctggccg
      661 tccagctgct gctgctcgcg ccagcttgac atccaggcgc cggatcgctt ggagggacgt
      721 gcaaagtagt cgccgacgaa caggccgagc tggcgaccgg ccgcgtcctt gatttcgaac
      781 gcgcgcgcgt cgggatgata aagagaaagg tcacgccgtt cggtggcgac gattccgaac
      841 agccgctgtg ccacatcgaa ggcggctgcg atcacgtttt ccagctgcag gtagggtttg
      901 atcaaaccct cgtcgatgtc gaatttttcc ttgcgcacct tctcggcgta gtagcgccaa
      961 tcccaaggcg ccagccggaa attgccaccc gcggcgacga tggtcttttg caagacatca
     1021 cgctcttggc gcgcgcgggc acgcgccggc cgccagacgt ccatcaggag ccggcgcacc
     1081 gcctttggcg ttttcgccat cgagaactcg agcgtggcgg ccgcggccgt gtcaaagcca
     1141 agcaggcgcg cccgctcggc ccgcagcgac aggatctcgg caacgagttt gcgattgtcg
     1201 gtctgcccgc cgttggcccc gcggctgatc cagccctgaa aggccttttc gcgcagatcg
     1261 cgccgcgccg aaaactgcag gaagggctct atgctggagc gcgccagcga gatggcgtac
     1321 ttgcccggca ggccgcgcgc gttcgccgtc tcggccgccg ccgcgcgcag ggcatcgggc
     1381 aggccgacaa ggtcctcggc agcgagcacc attaggaaag cctgttcgtc ggccaggaca
     1441 ttctggttga atttggtggc gagcttggca agtcgctggg cgatgacggc gagccggcgc
     1501 ctggcgccag ccttcaattg cgctcccgcc ctaacgaagg cccggtgata gcgctcgagc
     1561 acccggcgct gctcctcact gagctcgacg ctggctgctt gccgcgtcag ggcggcaacg
     1621 cgcttgaaca gcttggcgtc ctgatagatg cgcatgctgt gcttggccaa gcgcggggcg
     1681 atcttgcgtt cgatctcctc gatttcaggc gacgtatcgg tgccggacaa attgaagaac
     1741 accgaggcga cccggtccag tgtgcgacca ctgcgctcca gagcttcgat ggtgttggca
     1801 aacgtgggtt gtgcgcgctg attggcgatg gcggcgagtt cggtcaaatt gtgcgcaagc
     1861 gcccgatcga acgccggcag aaagtgctcg ctgcgcaggc gcgcaaaggg aggcagctcg
     1921 aagggcccgg tccatgcctc gagcaagggg ttgcgggcgg cggcggggcg ctcgaggcgc
     1981 tttacggctt ttttgcgccg cgcgggtttt gccataccgc ctctgcggcg tgtctgcgag
     2041 gctgccttct gcgccatgct ccgtccccat gcgagccaac tcgtgacttt ggacctatga
     2101 cgcctcttgg cggcaatgtc gagggagctg gcgggggcgc ggcagacctc aggaatagat
     2161 catcttgcgc gtcatgccgc catccacgat caattcggcg cccgtcacga agccggcggc
     2221 ggccgagagg agataagatg ccgcggccgc cacgtcgtcg ggctcgccga cgcgcccgac
     2281 cggatgctgc gcgtggtcgc tcgccttcag cgtgccatgc cggccggtat cgatccaccc
     2341 cggcgaaatg acgttgacgc gcacctcggg cgccagggtc atcgccaggg cgtgtgcgag
     2401 ggccaccagt ccgcccttgc tggcggagta ggcgagcgta ttcggctcgg acatgtgcgc
     2461 gcgcgtcgag ccgatcagga gcatgcttcc ccgcttctgg cgcaggtcgg ccgcgccgct
     2521 cttggccagc aggaaggcgg ccgtcagatt ggtggcgagc acccggttcc agtcctcgag
     2581 gctcgtctgc gccaaaggcg cgaatttgcc gatgccggcg ttggaaacga ttgcgtcgat
     2641 gcgcccgaag cgggtccgtg ccagggcaag gaggcgttcg atctccacct cgcttcccac
     2701 gtcgcattgg acggccgccg cgccgatcgt agccgcgacc ccctgcgcgg cggcggggtc
     2761 gatgtcggcg cagaggacct gccagtcctc ggcggccagc ctgcgcgcaa tgctggcccc
     2821 gatgccgtgg cccgccccgg taacgatcac agccttgccg cctacacgcc ctgccatgcc
     2881 gttcacaaat cagaaattct cggttcttgc gagggggttg cggacattgg gtaaaagagg
     2941 gccgtcaggc aggtttgccg gccgcggcca aatagttcac gtccgtatcg ctgccgagcg
     3001 accactcgtc ccgaagcggg ttgtaaaaaa gcccctgcag cgccggctcg cccagccccg
     3061 cggcgcgcag gtgctggcgc aactcttgcg gggtgacgaa cctgtcccag cgatgggtgc
     3121 cgaccggcag ccagcgtaac acgtattccg cgccgataat ggcgagtgcg taggccttga
     3181 gcgtgcgatt gatggtggag agcagcatca gtccgcccgg gcgcagcacg cgggcacagg
     3241 tggcaaggaa ggcgcccacg tccggcacat gctcgatgac ctcgagacag ataacggcgt
     3301 catagctgag gccttccccg gccaagtcct cgatgcggcc ggcacgatag tcgatcgcca
     3361 gcccttgcgc ggcggcgtgc tgccgcgccg cctcgatatt cgcctgcgcc ggatcaagcc
     3421 cggtgacgag cgcggcgagc cgcgccagcg gctccgcgat caggccaccg ccgcagccca
     3481 cgtc
//
LOCUS       DASTMT010000484         3515 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_2898313, whole genome shotgun sequence.
ACCESSION   DASTMT010000484 DASTMT010000000
VERSION     DASTMT010000484.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3515
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2898313"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..99
                     /locus_tag="VFR00_11090"
     CDS             <1..99
                     /locus_tag="VFR00_11090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEU0159854.1"
                     /translation="IGPLDEGMRVSFETEPDKRGKGPKAVNLQAAT"
     gene            237..551
                     /locus_tag="VFR00_11095"
     CDS             237..551
                     /locus_tag="VFR00_11095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0159855.1"
                     /translation="MTPFRPRLMGVVCVALGVAAASPAAAQCTTIGGKGTGGSLDSAR
                     FQAYEAMLQGTDLGMWANWMVSGAKVGVAPGYRVGGLRFKCGPGGMGQECRARATFCK
                     AA"
     gene            593..910
                     /locus_tag="VFR00_11100"
     CDS             593..910
                     /locus_tag="VFR00_11100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0159856.1"
                     /translation="MKRAEKLMQLSLGLAAAAAIALATGGAEAQCVNKAGQGTNTTEE
                     GAKSQAYEAVLQATDWGMWSVWMGQGMKYGSAPGYKVSNVKARCKKGGLGTECVVQAK
                     LCK"
     gene            1038..1298
                     /locus_tag="VFR00_11105"
     CDS             1038..1298
                     /locus_tag="VFR00_11105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0159857.1"
                     /translation="MKTLFGIALGGCALAALVLASGGPAEAKCGKASASGVGLGPEMA
                     KEMAKMNLAAGLEEKKMKAKGKVSYKCSGPFMSECKASQRAC"
     gene            1473..1823
                     /locus_tag="VFR00_11110"
     CDS             1473..1823
                     /locus_tag="VFR00_11110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0159858.1"
                     /translation="MTLKSLLILLGCLGLAASIGPVAAEEGRSGRVVTTADAAKVRAY
                     RKKRSPLEIDIYAQRRRGGYSYRVPDITSTYNTRNPPPYMHVRQTPSGPFDSGFWFDS
                     GCCGRNAGDAPYPH"
     gene            2018..2398
                     /locus_tag="VFR00_11115"
     CDS             2018..2398
                     /locus_tag="VFR00_11115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0159859.1"
                     /translation="MRCPTLFATLAAAALGLPTLGMTPALARCTTLAFSVNDYGKDGP
                     TKDAKDLLDKYIAKWTKDNNVTNYTVGKKDVSCELFLNFIVFDEHTCKASAQVCWPGE
                     GGPAPAASPAISPPPKAAAKRAAG"
     gene            complement(2406..>3515)
                     /locus_tag="VFR00_11120"
     CDS             complement(2406..>3515)
                     /locus_tag="VFR00_11120"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEU0159860.1"
                     /translation="PLLNARPLGNPEQVDALILALANAKKPVIVSGSGVIWSRAWNEM
                     QELVERAGVPFYTTPQGRGVVPDDHPLSYLSMRSTAFKDADLIIVLGTRMNYIISHAA
                     PPRFNADAKIARIDIDANEIATAPRQVDIGIVGDCKMVLNQILSGMKGKVDPDRFKDW
                     RQRLAAGAAQKLASPGANKPVEDGDIHPLRLCEEINAFIDRDAILVVDGQEVLNFGRQ
                     SMQTHAPGHRLNSGPFGTMGVGLPFALGAKLAKPDKQVVCLHGDGSFGLNAMELDTAV
                     RHNLPVLVVISLNGGWTADPKREKPGRELGYTRYDTMAEALGCYGDYVDKVEDIRPAL
                     ERAQREVDKGKVAVLNVRTDYRARAGTLQFAQYST"
BASE COUNT          638 a         1078 c         1206 g          593 t
ORIGIN      
        1 attggacctc tggatgaggg catgcgcgtg tcgttcgaaa ccgaacccga caagcgcggc
       61 aaggggccaa aggcggtcaa tctgcaggcc gcaacgtgag gagccgatgg ccgtcacccc
      121 gcggcaacgt gtgacggaaa atctgtgggt ggcagcccct tagacggccg ccgccagtgg
      181 gcaagctagg cgatctcggg cagtctgccc gggatcgctt tcttttgggg gatgccatga
      241 caccgttcag gccgaggctc atgggcgttg tctgcgtcgc ccttggcgtc gccgcggcga
      301 gcccggcggc ggcacaatgc accaccatcg gtggcaaagg caccggcggc tccctcgata
      361 gcgcccggtt ccaggcctat gaggccatgc tgcagggcac cgaccttggc atgtgggcga
      421 actggatggt cagcggcgcc aaggtaggcg tggcccccgg ctatcgggtc ggcggcctca
      481 ggttcaagtg tggccccggc ggcatgggcc aggagtgccg cgccagagcc acgttttgca
      541 aagcggcctg agccaaaacc agcgattccc ttgagagtga aggagaacga cgatgaagcg
      601 cgcagaaaag ctgatgcaac tgagccttgg gctggcggcg gcggccgcca tagcgctggc
      661 caccgggggc gccgaggcgc agtgcgtgaa caaggcaggg cagggcacca acacgaccga
      721 agagggcgcc aagagccagg cctatgaggc ggtgctgcag gcgaccgact ggggcatgtg
      781 gtcggtgtgg atgggccagg gcatgaaata cggctccgcg ccgggttata aggtcagcaa
      841 cgtcaaggcg cgctgcaaaa aaggtgggct tggcaccgaa tgcgtggtgc aggcgaagct
      901 ctgcaagtag ggcccgagcg taaccgtgac tgatcggcca cggaccgtgc aaaagcgccg
      961 ccgaaacctg cctgttgctc ggaaatgcgg gtcggtcggg ccataatgca gcgtccatac
     1021 ttgctggagg tgaggctatg aagacgttgt tcgggattgc gcttggcgga tgtgcgctgg
     1081 cggccctggt gcttgcgagc ggtggtccgg ccgaggcgaa gtgcggcaag gcgagcgcct
     1141 cgggcgtcgg cctgggaccg gagatggcca aggaaatggc caaaatgaat ctggccgccg
     1201 gcctggaaga aaagaagatg aaggccaaag gcaaggtcag ctacaagtgc tcaggcccct
     1261 tcatgtcgga gtgcaaggcc agccaacgcg cctgctagct aaacgttttc aatggtttgc
     1321 ggggccgacc ggtatgccgg acggtccctt tttagcctcg gaggtcagca ccggcgcccc
     1381 cgagcacaca atttacccct tggttgcctt cctacactag actggcgggc agcccctcgg
     1441 catgctgctc tagcacaaca cgaaagacca ccatgacgct caagtctctc ctgatcttgc
     1501 tgggttgtct tggccttgcc gcgagcatcg ggccggtggc tgccgaggag gggcgcagcg
     1561 gacgcgtggt gaccacggcg gacgccgcaa aagtgcgcgc ctaccgcaag aagcgctcgc
     1621 cgctcgagat tgacatctat gcccagcgcc ggcgcggcgg ctattcctat cgcgtgcccg
     1681 acatcacctc gacctacaac acgcgcaacc caccccccta catgcacgtg cgccaaaccc
     1741 cgagcggtcc cttcgatagc ggcttctggt tcgattccgg ttgttgcggg cgcaacgccg
     1801 gcgacgcgcc ctacccgcac taaagacgcg tgcgggcggc gacgcgctcg cctcctccga
     1861 ccgaggcgtg ctcgggctgc aaccctggcg cgcttgcgcg tgcgggagcg catttttcgg
     1921 tagaaaaggg ggctgagcgc ggcgcgcctg ctctaggcag cctgtgcgcg ggggctatag
     1981 taggcaccga cccgcaagaa aaattggagc gtcttcaatg cgctgcccca cgctgtttgc
     2041 cacgcttgcc gcggccgccc tcggcctgcc taccttgggc atgaccccgg cgctggcgcg
     2101 ctgcacgacg ctcgccttct cggtcaacga ctacggcaag gacgggccga ccaaggacgc
     2161 caaggaccta ctcgacaagt acatcgccaa gtggaccaag gacaacaacg tcaccaatta
     2221 cacggtgggc aagaaagacg tcagctgcga gctgttcctc aactttatcg tcttcgacga
     2281 gcacacctgc aaggcgagtg cccaggtgtg ctggccgggc gagggtggac cggcaccagc
     2341 ggcgtccccc gcaataagcc cgccgccgaa agcggcggcc aagagggctg ccggctagcg
     2401 attttttacg tcgaatactg tgcgaattgc agcgtgccgg cgcgggcgcg ataatcggtg
     2461 cgcacgttga ggaccgccac tttgcccttg tccacctcgc gctgggcccg ctcaagggcc
     2521 gggcggatgt cctcgacctt gtcgacgtag tcgccatagc agccgagcgc ctccgccatc
     2581 gtgtcgtagc gcgtgtagcc gagctcccgg ccgggttttt cgcgtttggg atctgcggtc
     2641 cagccgccgt tgaggctgat gacgacgagc acgggcaagt tgtggcgcac ggccgtatcg
     2701 agctccatgg cgttgagccc gaacgagcca tccccgtgta gacagacgac ctgtttgtcg
     2761 ggcttggcca gcttggcgcc gagcgcgaag gggaggccga cgcccatggt gccgaagggg
     2821 cccgagttca gacgatggcc cggagcatgc gtctgcatgg actggcggcc aaagttcaag
     2881 acctcctgtc catcgacgac gaggatggcg tcgcgatcga tgaaggcgtt gatctcctcg
     2941 cacagccgca agggatgaat gtcgccgtcc tcgacgggct tgttcgcccc cggcgaggcc
     3001 agcttttggg ccgcgcccgc ggcgagccgc tggcgccaat ccttgaagcg gtcggggtca
     3061 accttgccct tcatgccgct caggatctga ttgagcacca tcttgcagtc gccgacgatg
     3121 ccgatgtcga cctggcgcgg tgcggtggcg atctcgttgg cgtcgatatc gatgcgggcg
     3181 atcttggcat ccgcgttgaa ccgcggcggt gccgcgtggc tgatgatgta gttcatgcgc
     3241 gtgccgagca cgatgatgag atcggcatct ttgaaggcgg tggagcgcat ggacaggtag
     3301 gagaggggat gatcgtcggg cactacgccg cgaccttgtg gcgtcgtgta gaaggggacg
     3361 ccggcgcgtt cgacgagctc ctgcatttcg ttccaggcgc gtgaccagat cacgccgctg
     3421 ccggagacga tcaccggctt tttggcattt gccaaagcga ggatcagggc gtcgacctgc
     3481 tcgggattac ccagcggccg cgcgttgagg agcgg
//
LOCUS       DASTMT010000485         2054 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_934796, whole genome shotgun sequence.
ACCESSION   DASTMT010000485 DASTMT010000000
VERSION     DASTMT010000485.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2054
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_934796"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            33..332
                     /gene="rpsU"
                     /locus_tag="VFR00_11125"
     CDS             33..332
                     /gene="rpsU"
                     /locus_tag="VFR00_11125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013529653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEU0159861.1"
                     /translation="MLVQVRDNNVDQALKALKKKMQREGIFREMKLRNYYEKPSEKRV
                     REQAEAVRRARKLARKRAQREGLLPGKAATPKPGAAKTGGRPGGPPRSGGFGGPR"
     gene            complement(377..1189)
                     /locus_tag="VFR00_11130"
     CDS             complement(377..1189)
                     /locus_tag="VFR00_11130"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU0159862.1"
                     /translation="MWVVVKLAVAALCGCLLLLLVAYFGQRKLIYFPDRSRVLPGQLG
                     LPGVEERVLKTPDGERLIAWYAKARPGEPTLLYFHGNGGGLRERAERIRQFTGEGWGV
                     YMMAYRGYAGSSGTPTEAANVADARLAYGALLLEGVAAETVIAYGESLGTNLATRIAV
                     ERKVGGLILEAPYTSIAKLGGQIYPFLPVDLLLIDRYETDKVIAQVKVPTLILHGQRD
                     RTVPVGMGREIASLARAPAQLVVLDNAGHSDLFRDGNDALGVVREWLRTLAR"
     gene            complement(1251..>2054)
                     /locus_tag="VFR00_11135"
     CDS             complement(1251..>2054)
                     /locus_tag="VFR00_11135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013418975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HEU0159863.1"
                     /translation="DREQASVTVAGRETMIRPYPISIEWPPSALAGQPPVAECREAVR
                     RRFALAPTTRIAVGIERFDYTKGILDRMHAIDDLLRRQPAWKGRLVFIQAAAPTRGKL
                     AAYNALQCEAERLAEDINARHGSASYKPIVLVVRHHGPDEVFELFRAADVCVVSSLHD
                     GMNLVAKEFVASRDDEQGVLILSTFAGAARELSEALMVNPYDTYAMGEALEQALLMPP
                     AEQRERLRLMRDLVRVRNVYRWAGQMLLDAARLRNQQRIVASMVAETGA"
BASE COUNT          358 a          664 c          723 g          309 t
ORIGIN      
        1 ggcacaggcc ctaggcacaa caggaacacc gcttgctggt tcaggttcgc gacaataacg
       61 ttgatcaagc cctcaaggcg ctgaagaaga agatgcagcg cgaggggatt ttccgcgaga
      121 tgaagctgcg gaattattac gagaaacctt ccgagaaacg ggtccgagag caggctgagg
      181 cggtgcgccg cgcgcgcaag cttgcccgca agcgtgccca gcgggaaggt ctgttgcccg
      241 gcaaggcggc gacgcccaaa cccggggcag caaaaacggg cggtcgcccg ggcggcccgc
      301 cgcgcagcgg cggcttcggc ggcccgcgct aaccccattg aggcacgtct ggctcgaggc
      361 ccggcgcgtg cgccggtcag cgcgcgagcg tgcgcaacca ctcgcgcacg acgccgaggg
      421 catcgttacc gtcgcggaaa agatcgctgt gcccggcatt gtctaacacg acaagctgcg
      481 ctggcgcccg cgccaggctg gcgatctcac ggcccatgcc gacaggcacc gtgcggtcgc
      541 gctggccgtg caggatcagg gtgggcactt tgacttgggc gatgaccttg tccgtctcgt
      601 agcggtcgat cagcaggagg tcgacgggca ggaagggata gatttgaccg ccgagcttgg
      661 cgatcgaggt atagggtgcc tcgaggatca ggccgccgac tttgcgctcg acagcgatcc
      721 gggtcgccaa attggtgcca agcgactcgc cgtaggcgat gaccgtttcg gcggcgacac
      781 cttccagcag caaggcgccg taggcgaggc gcgcgtcggc gacgttggcg gcctcggtgg
      841 gcgtgccgct gctgccggca tagccgcggt aggccatcat gtagacgccc caaccttccc
      901 cggtaaattg ccggatgcgc tcggcgcgct cgcgcagccc accgccgtta ccgtgaaaat
      961 agagaagcgt cggctccccc gggcgggcct tggcgtacca ggcgatcagc cgctcgccgt
     1021 cgggcgtttt cagcacgcgc tcctcgacgc cggggaggcc gagctggccc ggcagcacgc
     1081 ggctgcgatc gggaaagtag atgagcttgc gctggccgaa ataggcaacg aggaggagca
     1141 gcaagcaccc gcacagcgcg gcaacggcca gtttgacgac gacccacatg cccgccttct
     1201 cgctcggccc ccttgggcgg tcaagcgcca gcgcgggcgg tgcgaccggc tcaggccccg
     1261 gtttcagcga ccatgctggc aacgatgcgc tgctggttgc gcagccgggc cgcgtcgagc
     1321 agcatttggc cggcccagcg gtagacgttg cgcacgcgca cgaggtcgcg catgaggcgc
     1381 aggcgctcgc gctgctcggc gggcggcatc aacagcgcct gctccaaggc ctcacccatg
     1441 gcgtaggtgt catagggatt gaccatcaag gcttcggaca gctcgcgcgc cgcgcccgcg
     1501 aaggtcgaca agatgagcac gccctgctcg tcatcacgcg aggccacgaa ttccttggcc
     1561 accaggttca tgccgtcgtg caggctcgag accacacaca cgtcggcggc gcggaagagc
     1621 tcgaacacct cgtcgggacc atgatggcga acgaccagca cgatcggctt gtagcttgcg
     1681 ctgccgtgac gcgcgttgat gtcctccgcc aatcgctctg cttcgcactg cagcgcattg
     1741 taggctgcta gcttgccgcg cgtgggggcg gcagcttgga taaacaccag ccggcctttc
     1801 catgcgggct ggcgccggag caggtcgtcg atggcgtgca tgcgatcgag gatgcccttg
     1861 gtgtaatcga acctttcgat gccgacggcg atgcgggtgg tgggcgccag ggcaaagcgg
     1921 cgccggaccg cctcgcggca ctcggcaacc gggggttggc cggcaagcgc cgaggggggc
     1981 cactcgatgg aaatcggata ggggcggatc atggtttcgc gcccggccac ggtgaccgag
     2041 gcctgctcgc gatc
//
LOCUS       DASTMT010000486        13208 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_2899132, whole genome shotgun sequence.
ACCESSION   DASTMT010000486 DASTMT010000000
VERSION     DASTMT010000486.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 13208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13208
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2899132"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..899)
                     /locus_tag="VFR00_11140"
     CDS             complement(<1..899)
                     /locus_tag="VFR00_11140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012169221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HEU0159864.1"
                     /translation="MAKVIGIDLGTTNSCVAVMEGSKPKVLENAEGSNTTPSIVAFTD
                     DGERLVGLPAKRQGVTNPMNTFFAIKRLIGRNASDPVVEKDKGMVPYEIVKGPTGDAW
                     VRAHGKDYSPQQISAFILQKMKEAAEQHLGEKVEKAVITVPAYFNDAQRQATKDAGKI
                     AGLEVLRIINEPTAAALAYGLEKNDGKKIAVYDLGGGTFDVSILEIGDGVFEVKSTNG
                     DTFLGGEDFDLRIVDYLADEFKKEQSVDLRKDKLALQRLKEEAEKAKKELSSVPQYEV
                     NLPFISMNANGPLHLNIKLSRAKL"
     gene            1100..1741
                     /locus_tag="VFR00_11145"
     CDS             1100..1741
                     /locus_tag="VFR00_11145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009466531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate-dependent dioxygenase AlkB"
                     /protein_id="HEU0159865.1"
                     /translation="MYSSHKIAAGRAPPAGFRHLRGHFGTAEQALLLAEIYTVIRAAP
                     LFVPRMPRSGRAFSVRMSNCGALGWVSDQAGGYRYQAHHPETGQAWPPIPARLLALWD
                     ELAPGQPRPEACLINYYAASAKMGTHRDADEAAKTVPVVSVSLGDAAVFHVGGLERGD
                     RKHRLLLRSGDVVVLGGAARLAYHGIDRIAPGSSALLAEGGRLNLTLRRVNLP"
     gene            complement(1767..2453)
                     /gene="grpE"
                     /locus_tag="VFR00_11150"
     CDS             complement(1767..2453)
                     /gene="grpE"
                     /locus_tag="VFR00_11150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEU0159866.1"
                     /translation="MSAPDRMMHHPSPDESDPNRPSSDTAPSGPAAEAGPAAGSEAEG
                     QRSVHDVIAALQAEVADYKDRWLRAQAEIENVRKRYEREKDETAKYAITKFARDIVNV
                     GDNFQRAIDAVPAGAAEREPALKSFLDGVTLTERELLNILERHGIKRLNPKDEPFNPH
                     VHQAMMEVQRNDVAPGTIVQVFQPGSIIEDRVLRPARVVVAKAEPRSAEARPAAANDD
                     SAPNGTGGTG"
     gene            complement(2545..3636)
                     /gene="hrcA"
                     /locus_tag="VFR00_11155"
     CDS             complement(2545..3636)
                     /gene="hrcA"
                     /locus_tag="VFR00_11155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013420993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEU0159867.1"
                     /translation="MHSDKPISRSGSLEALSDLQKLNDRSRTIFRQIVESYLATGDPV
                     GSRNLARNLPMTLSPASIRNVMSDLEHLGYIYAPHTSAGRLPTEAGLRLFVDGLLEIG
                     DLTDEERRQIEAQIGVKREKSLQQVLSEAGEMISGLSHCAGLVISDRQIGKLKHIEFL
                     QLEPGKALVILVGDDQSVENRVISLPAGLPPSSLTEAANYLNARIRGLTLGEAKTRVE
                     AELASAKAELDALTQKIVEAGLATWSGALGEQKSLIVRGQANLLKDVDGLEHLARIRQ
                     LFDDLEAKRELVQLLGLAERGEGVHIFIGSENKLFSLSGSSLIVAPFQDKQRQIVGVL
                     GVIGPTRLNYARIIPMVDYTARLVGRLLT"
     gene            4149..4862
                     /gene="rph"
                     /locus_tag="VFR00_11160"
     CDS             4149..4862
                     /gene="rph"
                     /locus_tag="VFR00_11160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013950301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HEU0159868.1"
                     /translation="MRPSKRKPDELRRVSIERAVSMHAEGSCLIKFGNTHVHCTASLE
                     ERMPPWLKGQGRGWVTAEYGMLPRATTDRTRREATAGHPSGRTQEIQRLIGRALRAVV
                     DLPKLGERQISIDCDVIQADGGTRTAAITGAWVALHDCLKWMQARDMIREGVLRDHVA
                     AVSCGLYKGTPVLDLDYPEDSGADADANFVMTGSGGIVEVQGTAETVPFTQANFDQLM
                     ALARKGIAELIELQKLTVA"
     gene            4879..5289
                     /locus_tag="VFR00_11165"
     CDS             4879..5289
                     /locus_tag="VFR00_11165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU0159869.1"
                     /translation="MLPSGILETVLYAKDLDGAERFYADALGLIPFGKAAGRHLFYRC
                     GNQVLLIFNPEATRLAPAPGALPVPAHGMDGEGHLCFRASGLELDTWRAALEAKGVVI
                     EADFEWPGGGRSIYFRDPAGNCLEFAEPRIWKLA"
     gene            5286..5906
                     /gene="rdgB"
                     /locus_tag="VFR00_11170"
     CDS             5286..5906
                     /gene="rdgB"
                     /locus_tag="VFR00_11170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HEU0159870.1"
                     /translation="MTRRLPPDTRLLVASHNAGKVREIIELVAPFGLAIATAAELGLP
                     EPAETGASFVANAELKAKAAASAAGLPALADDSGLEVMALGGAPGIYSARWAGPGKDF
                     GVAMQRVHDELARRGAWTPPGPFANFTAALCLAWPDGAAAAFEGKVDGRLVWPPRGAK
                     GFGYDPMFVPTGENLTFGEMEPDAKHAMSHRARAFERFVRACLGDA"
     gene            5899..7119
                     /gene="hemW"
                     /locus_tag="VFR00_11175"
     CDS             5899..7119
                     /gene="hemW"
                     /locus_tag="VFR00_11175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HEU0159871.1"
                     /translation="MPSGAFGVYLHWPFCASKCPYCDFNSHVRAGGIDEARFVRAYRR
                     ELRHWARLTPARRVGSIFFGGGTPSLMSPGAVGDLLDEIGALWAIDAEVEVTLEANPS
                     SVEAGRFSGYRAAGINRVSLGVQSLCDEALRGLGRLHTAAEAVAAIEVTRRTFPRFSF
                     DLIYARPRQTLAAWRAELAQALALAGQHLSLYQLTIEAGTPFAALSTAGKLVLPDAAR
                     AADFYQETQAATAKAGLPAYEISNHAAPGEECRHNLLYWRYGEYAGVGAGAHGRRVAS
                     DGRHATSTELHPERWLAAVEAGGCALLEDTLLTPAEAADEALLMGLRLSEGLDLADLH
                     RLAGVRPGAEAVARLARHGLIECEGTRIRVMPAARLILNAIVVELARALEPAAVAGAD
                     RSVVEEVEETRGHA"
     gene            complement(7078..8358)
                     /locus_tag="VFR00_11180"
     CDS             complement(7078..8358)
                     /locus_tag="VFR00_11180"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator"
                     /protein_id="HEU0159872.1"
                     /translation="MGGALAAAAARVGHRPLATLARVAAALLLVGCAANTPGPLATSA
                     TTPGSETGPKGAPVKVALLASLSAQGQPGIIGKALKQSGELALFDRDSPNLQLLIKDD
                     KGTPEGAAVAASDAIKDGASLIVGPLFAKSVAAVAPFARQAKVPVVAFSNDRQVAGRG
                     VYLLSFQPAPEIGRIVSYAVRQGKRRFAALIPDDAFGKLAGTAFRDAVERAGGTIVAL
                     ETYPASANGMLEPMRKVGTVLRAAQDSEGGQIDALFLPGGQENLERIARLMPQADIDA
                     SRVKVLGTGGMDYANAGHNAGLVGAWYPGPDPRGWEEFAQKFAKTYAAAPPRIASLAY
                     DAVSLAIALAGTANEAERYGAGALTRPSGFTGIDGSYRLNADGTAERGLAILEVQRIG
                     AGIIDPPPALGSAPATPGAAASGVASGLFNFFNN"
     gene            8524..9450
                     /gene="rsmI"
                     /locus_tag="VFR00_11185"
     CDS             8524..9450
                     /gene="rsmI"
                     /locus_tag="VFR00_11185"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013950316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEU0159873.1"
                     /translation="MTRPAQPLPGAGAATAERAGTLLAAELAQALPPGLYIVATPIGN
                     LGDISLRALATLARADLIYCEDTRHSRTLMAHFAIARPLRAYHEHNAERERPRLIGEL
                     TAGRSVALISDAGTPLISDPGYKLVREALAAGIRVTSLPGASALTAALTCCGLATDTV
                     LFAGFLPPKQSARRTRLAELAGTPATLVLFEAPSRLAASLADIGQALPQRPMAVAREL
                     TKLHEEVRTGDAHELAQWASQSAPRGEMVIVVGPPATAPSVADTAIAAHLRDLLKDMS
                     VRDAARATAEALNVSKARAYELALALKKDEDN"
     gene            9597..10541
                     /gene="gshB"
                     /locus_tag="VFR00_11190"
     CDS             9597..10541
                     /gene="gshB"
                     /locus_tag="VFR00_11190"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013419281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HEU0159874.1"
                     /translation="MPLKVACQMDPIDRIDIRGDSTFALLLEAQQRGHQLFYYTPPHL
                     SLLAGRLIARGTTLNVEDRVGNHYRVGDEREEDLSRMDVVLLRQDPPFDMSYITTTHL
                     LERIHPKTLVVNDPAHVRNAPEKVFVLDFLDLMPPTLVTRHLEQVRAFRAVHKDIILK
                     PLYGNGGASVFRLTEDDTNLNSLVELFQTVFREPFMVQQYRPEVRAGDKRIILVDGDV
                     VGAINRVPAADETRSNMHVGGVATATELTQRDLEICRRLGPELKRRGLLFTGIDVIGP
                     YLTEINVTSPTGIRQVKAFGGPDIAALIWDAIDAKVRR"
     gene            complement(10716..12251)
                     /locus_tag="VFR00_11195"
     CDS             complement(10716..12251)
                     /locus_tag="VFR00_11195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015932923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase family protein"
                     /protein_id="HEU0159875.1"
                     /translation="MQDSPQQSTPRTLRLNPRDNILLAVDPVERGSTIDGVTARARIL
                     RGHKMAAQPIAKGQPVLKFGQIIGFASEDIAPGSHVHTQNCSFAEFERDYAFAQDARE
                     EVLLPAELRATFAGFRRSSGKIGTRNYIGVLTSVNCSASVARFMAEAVSRSGLLEAYP
                     NVDGVVSFVHGTGCGLAAKGEGYDVLERTQWGYAGHPNLAATLLVGLGCEVFQIARLK
                     ERYGLVEGDNFRTMTIQDTGGTKKTIEAGVARIKEMLPRANAVRRETVPASEITLALQ
                     CGGSDGYSGITANPALGAAADLLVRHGGTAVLSETPEIYGAEHLLTRRARNRQVGEKL
                     VEIIRWWQDYCARNDGSMDNNPSPGNKAGGLTTILEKSLGAAAKGGTTTLNEVYRYAE
                     PVTAKGFVYMDTPGYDPVAATGQVAGGCNLLCFTTGRGSAYGCKPTPSIKLATNHDVY
                     RRMLDDMDIDCGDILDGVSIDAKGQEIFDAILRVASGEKTKSELLGYGDNEFVPWQIG
                     ATM"
     gene            12399..>13208
                     /locus_tag="VFR00_11200"
     CDS             12399..>13208
                     /locus_tag="VFR00_11200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008139412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU0159876.1"
                     /translation="MNLASLLKARAIDAKPIRVGQIGAGKFGTMFLSQVRLTPGMHLA
                     GLADLVPQRAKDRLSEVGWPAAQLDVASLNDAIKTGRTFLTDDAMAIVAHPEIEVVIE
                     ATGDPATGIRLCQAAIQHGKHVVMVNVEADALAGPLLARRAREAGVVYSLAWGDQPAL
                     ICEHVDWARTCGFEVIAAGKGTRYHPSYHQSTPDTVWNILDAYLKITDRSHINPKMFN
                     SFIDGTKSGIEMTAVCNATGLLPQTNGLGFPPSSRFDLAEVCKPRSDGGSLE"
BASE COUNT         2059 a         4438 c         4417 g         2294 t
ORIGIN      
        1 agcttggcgc ggctgagctt gatgttgagg tgcagcggcc cgttggcgtt catcgaaatg
       61 aagggcaggt tcacctcgta ctgcgggacc gaggacagct ccttcttggc cttctcggcc
      121 tcttccttga ggcgctggag ggccagcttg tccttacgca ggtcgacgga ctgttccttt
      181 ttgaactcgt cggccaggta gtcgacgatc cgcaggtcga agtcctcacc gcccaggaag
      241 gtgtcgccgt tggtcgactt cacctcgaag acgccgtcgc cgatctccag gatcgacacg
      301 tcgaaggtgc cgccgccgag gtcgtagacc gcgatcttct tgccgtcgtt cttctcgagg
      361 ccgtaggcca gcgcggccgc cgtcggctcg ttgatgatgc gcagcacttc gaggccggcg
      421 atcttgccgg cgtccttggt ggcctggcgc tgggcgtcgt tgaagtaggc cgggacggtg
      481 atgaccgcct tctcgacctt ctcgccgagg tgctgctcgg cggcttcctt catcttttgc
      541 aggatgaagg ccgagatctg ctggggcgaa tagtccttgc cgtgggcgcg cacccaggcg
      601 tcgccggtcg ggcctttgac gatctcgtag ggcaccatgc ccttgtcctt ctcgaccacc
      661 ggatcggagg cgttacggcc gatcaggcgc ttgatggcga agaacgtatt catgggattg
      721 gtcacccctt ggcgcttggc cggcaggccg accaggcgtt cgccatcgtc cgtgaaagca
      781 acaatggatg gggtggtgtt ggacccttca gcgttttcga gcaccttggg cttggaaccc
      841 tccatgaccg cgacgcaaga gttggtggtg ccgaggtcga tgccgatcac tttggccata
      901 tgctctttgt cctctctttt gcggcagacc gtccggaccc aaaaactcgg cgtccggcgc
      961 ccatggccca gattggagcc ttgagaacgg tcccctagag ctgcgattgc ttgtctggcg
     1021 ttatatagtg gggccctttt ggggctgcaa cggtcgcgag ccaacgcccg cgcggcaatt
     1081 gtgacgaagc ctttgatcga tgtacagcag ccacaaaatc gccgctgggc gggcgccacc
     1141 cgcgggcttt cgccatttgc ggggccattt cgggacggcc gaacaggccc tgctgctggc
     1201 cgaaatttac accgtgattc gggccgcccc cctgtttgtg ccgaggatgc cccgcagcgg
     1261 ccgcgccttt tcggtccgca tgagcaactg cggggcgctg ggctgggtct cggaccaggc
     1321 ggggggctat cgctatcagg cgcaccaccc ggaaaccggg caggcctggc ccccgatccc
     1381 ggcgcgcctg ctggcgctct gggacgagct cgcccctggg cagccgcgcc cggaggcgtg
     1441 tctcatcaac tactacgccg cgagcgccaa aatggggacg caccgggatg cggacgaggc
     1501 ggcaaagacc gtgcccgtcg tttccgtttc gctgggcgat gctgccgtct ttcacgtggg
     1561 cgggctcgag cgcggcgatc gcaaacaccg cttgctcttg cgctcgggcg atgtcgtggt
     1621 gctgggggga gccgcgcgcc ttgcctatca cggcatcgat cgcatcgcgc cgggcagctc
     1681 ggcgctgttg gccgagggcg gccgcctgaa cctgaccctg cgtcgggtca acctgcccta
     1741 aacagccccg cacgcaaccc tgccaatcaa ccggtgccgc cggtgccatt gggcgcgctg
     1801 tcgtcgttgg cggccgccgg gcgcgcctcg gcggatctcg gctcggcctt ggcgaccacc
     1861 accctggccg gccgcagcac gcgatcctcg atgatagagc cgggctggaa cacctgcacg
     1921 atggtgccgg gcgcaacatc gttgcgctgc acctccatca tggcctggtg aacgtgcggg
     1981 ttgaagggct cgtccttggg attgaggcgc ttgatgccgt gccgctccag gatgttgagc
     2041 agctcgcgct cggtcagcgt gacgccgtcc aggaagctct tcaaggcggg ctcccgttcg
     2101 gcggcgccgg cgggaaccgc atcgatcgcg cgctgaaagt tgtcgcccac gttgacgata
     2161 tcgcgggcga atttggtgat ggcgtacttg gcggtctcgt ccttttcgcg ctcgtagcgc
     2221 ttgcgcacgt tttcgatctc cgcctgggcg cgcagccacc tgtccttata gtcggcaacc
     2281 tcggcctgca gcgcggcgat gacgtcgtgc acgctgcgtt ggccctctgc ttcgctgccc
     2341 gccgccgggc ctgcctcggc agcggggccg ctcggcgccg tgtcggagct cgggcggttc
     2401 gggtcggact cgtcgggcga tgggtgatgc atcatgcgat ccggagctga caacctacaa
     2461 accttgccga tggcagcctt acgaccagag cgagaccaca ggcaatgggg gttcgtgccg
     2521 atatcgtgca gcacgccaaa aaaatcaagt cagaaggcga cccaccaacc tggccgtata
     2581 gtccaccatc ggaatgatac gcgcataatt gagacgcgtg ggaccgatca ccccgaggac
     2641 gcccacgatc tggcgctgct tatcctgaaa gggggccacg atcagggagg agcccgacag
     2701 cgagaacagc ttattctccg aaccgatgaa aatgtggacc ccctcgccgc gttcggcgag
     2761 ccccagcagc tgcacaagct cgcgcttggc ctcgagatcg tcgaacagct ggcggatgcg
     2821 cgccaggtgc tccaggccat cgacgtcctt gagcagattg gcttggccgc gcacgatcaa
     2881 gcttttctgc tcaccgagcg cgcccgacca agtggcaagc ccggcctcga cgatcttctg
     2941 cgtcaaggca tcgagctcgg ccttggcgga ggcgagctcc gcctccacac gcgtcttggc
     3001 ttcgcccagg gtcaggccgc ggatgcgggc gttgagatag ttggcggctt ccgtcaggct
     3061 ggaggggggc aaccccgccg gcaagctgat cacgcgattt tccacgctct ggtcgtcgcc
     3121 gaccaaaatc accagcgcct tgccgggctc gagttgtagg aactcgatgt gcttgagctt
     3181 gccgatctgg cggtcgctga tcaccagccc ggcgcagtgg gagagacccg agatcatttc
     3241 gccggcctcg ctcagcacct gctgcagcga cttttcgcgc ttgaccccga tctgcgcctc
     3301 gatctggcgg cgttcctcgt cggtgaggtc gccgatctcg agcagcccgt cgacgaacag
     3361 ccgcaggccg gcctcggtgg gcaggcggcc ggccgaagtg tggggcgcat agatgtagcc
     3421 gaggtgctcc aaatccgaca tcacgttgcg gatcgaggcc ggcgacaggg tcatgggcag
     3481 gttgcgcgcg aggttgcgcg agccgaccgg atcccccgtc gccaaatagc tctcgacgat
     3541 ctggcgaaag atggtgcgcg agcggtcgtt gagcttttgc aggtccgaga gggcctcgag
     3601 cgacccggaa cggctgatcg gcttgtcgga gtgcatgctc atgcagatgt gaggttgcgc
     3661 ggggcccgag tctagggaag agcgcccgcc ctcgtcaaac aaagctgccg ttctaagcca
     3721 agggggtgtg gacttggcgt gctcgccgcg gcctggcccg ctgccgtcct gccaccccgc
     3781 gccggcgatg tcgcggagcg cggcggcggg aaatgccaag cgacctgagg gtgctagagc
     3841 tcggccccca acatgggccg agggggttta gcgcgcgtgg cgggcgggag cagcggcggc
     3901 tgacggccgg gcgccgtcgc ggcgaggccg ggccggccgg cgggcgcgcg ggtgggctgc
     3961 ccacggccgt gccatcgctc ggcgcgggcc tgcgtgtccc gtccgcgccg cccggcggcg
     4021 ccggcgcgcc ccgcctcggc gtctctttcg gcaggcgtgc ggcccgggcc gaagtgcttc
     4081 caatgcgcgc gatctcgctt tagtgtgcgc cccacaaatt tcctcggcta gagatcgaag
     4141 tgcgcccgat gcgtccctcc aaacgcaagc ccgacgaact acgccgcgtc agcatcgagc
     4201 gcgcggtgtc gatgcacgcg gaaggctcct gcctgatcaa gttcggcaac acccacgtgc
     4261 actgcacggc cagcctcgag gagcgcatgc cgccctggct caaggggcag gggcgaggct
     4321 gggtgacggc tgaatacggc atgctgccgc gcgccaccac cgaccgcacg cggcgcgaag
     4381 ccaccgccgg ccacccctcc ggtcgcaccc aggagatcca gcgtctgatc gggcgcgcgc
     4441 tgcgcgccgt ggtcgatctt cccaagctcg gcgagcggca gatttccatc gactgcgacg
     4501 tcatccaggc cgatggcggc acgcgcacgg ccgcgattac cggcgcctgg gtggctttgc
     4561 acgattgcct gaagtggatg caggcgcgcg acatgatccg cgagggcgtg ctgcgcgacc
     4621 atgtggcggc cgtctcctgc gggctctata agggcacgcc ggtgctcgac ctcgattacc
     4681 ctgaggattc cggcgccgac gccgacgcca acttcgtcat gacgggctcc ggcggcattg
     4741 tagaagtgca gggcacggcc gaaaccgtgc cgtttacgca agcaaacttc gaccagttga
     4801 tggcgttggc gcgcaagggc atcgcggaac tgatcgagct gcagaagctg accgtggcct
     4861 aagccaagcg gagagccaat gcttccgtcc ggcattctcg agaccgtgct ctatgccaag
     4921 gacctcgatg gcgccgagcg cttctatgcg gacgcgctgg ggctcattcc cttcggcaag
     4981 gcggcaggac ggcacctgtt ctaccgctgc ggcaaccagg tgctgctgat cttcaatccc
     5041 gaggccaccc gcctcgcccc ggcgcccggc gccttaccgg tgccggcgca cgggatggac
     5101 ggcgaaggcc atctttgttt tcgcgccagc ggcctggaac tcgatacctg gcgggctgcg
     5161 ctcgaggcca aaggcgttgt catcgaggcc gatttcgagt ggcccggggg cggccgctcc
     5221 atttactttc gcgacccggc cggcaattgc ctggaattcg ccgagccgcg catctggaaa
     5281 ctggcatgac gcgccgccta ccgcccgata cccgcctcct cgtcgccagc cacaatgccg
     5341 gcaaggtccg cgagatcatc gagctggtgg cgccgtttgg tctggcaatc gccaccgccg
     5401 ccgaacttgg cctgcccgaa ccggccgaga ccggggcaag cttcgtggcc aatgccgagc
     5461 tgaaggcgaa ggcggcggcc agcgccgccg gcctgcccgc gctggccgat gattccgggc
     5521 tggaggtgat ggccttgggc ggggcgccgg gcatctattc ggcccgctgg gccgggcccg
     5581 gcaaggactt tggcgttgcc atgcaacgcg tgcatgatga gctggcgcgc cgcggcgcct
     5641 ggacgccccc ggggcccttt gccaatttca cggcggcgct ctgcctggct tggcccgatg
     5701 gcgcggcggc cgccttcgag ggcaaggtcg atggccggct ggtgtggccg ccgcgcggag
     5761 ccaagggttt cggctatgat ccgatgttcg tgccgaccgg cgagaattta accttcggcg
     5821 aaatggagcc tgacgccaag catgccatgt cccaccgcgc ccgcgccttc gagcgcttcg
     5881 tgcgtgcctg tctcggcgat gcctagcggc gccttcgggg tctatctgca ttggccgttc
     5941 tgcgcctcga agtgccccta ttgcgatttc aatagccacg tacgcgccgg cggcatcgat
     6001 gaggcacgct tcgtgcgcgc ctaccggcgc gaattgcgcc attgggcccg gctcaccccc
     6061 gctcgccgcg tcggcagcat ttttttcggt ggcggcacgc cgtcgctgat gtcgccgggc
     6121 gccgtcggcg acctgctcga tgaaatcggc gcgctgtggg ccattgatgc cgaggtcgag
     6181 gtgacgctgg aggccaatcc ctcgagtgtg gaggcgggcc gcttcagcgg ctaccgcgcc
     6241 gcgggcatca accgcgtctc gcttggcgtg cagtcgctgt gtgatgaagc gttgcgcggg
     6301 ctgggtcgtc tgcataccgc ggccgaggcg gtggctgcca tcgaggtgac gcgtcgcacg
     6361 tttccgcgct tttccttcga cctcatctat gcccgacccc ggcaaacgct tgcggcctgg
     6421 cgcgccgagc tcgcgcaggc gcttgcgctt gccggccagc acctgtcgct ttatcagctg
     6481 acaatcgaag cgggcacgcc gtttgccgcc cttagcaccg cgggcaagtt ggtccttccg
     6541 gatgcggccc gggccgcgga cttctaccaa gagacgcagg cggcgaccgc taaggccggg
     6601 ctacccgcct acgagatttc caatcatgcc gcgcccggcg aggaatgccg gcacaacctt
     6661 ctctactggc gttacggcga gtatgccggt gttggcgcgg gcgcccatgg ccgccgcgtg
     6721 gccagcgacg ggcgccacgc caccagcacc gagttgcacc ccgagcgctg gctcgccgcg
     6781 gtggaagctg gcggctgcgc gctgctcgag gacaccttgc tgacgcccgc cgaagcggcc
     6841 gatgaggcgc tgctgatggg gttgcggctg agcgaggggc tcgacttggc ggacctgcat
     6901 cgcctggccg gcgtgcgacc gggcgccgag gccgtcgcgc gcttggcccg gcacgggctc
     6961 atcgagtgcg aagggacgcg catccgggtc atgcccgccg cgcgcctcat tctcaacgcc
     7021 atcgtcgtgg agttggcgcg cgccttggag ccggccgctg ttgccggggc tgaccgctca
     7081 gttgttgaag aagttgaaga gacccgaggc cacgcctgaa gcggccgctc ccggcgtggc
     7141 gggcgcggaa cccaacgccg gcggcgggtc gatgatgccc gcgcctatcc tttgcacctc
     7201 gagaatggca agcccgcgct cggcggtgcc atcggcgttt agccgatagc tgccatcaat
     7261 gcctgtgaaa ccgctcgggc gcgtgagcgc gccggcacca tagcgctcgg cctcgttggc
     7321 cgtaccggcg agcgcaatcg ccaagctaac ggcgtcatag gcgaggctgg cgatccgcgg
     7381 cggcgccgcg gcgtaggtct tcgcgaactt ctgggcaaac tcctcccaac cgcgcggatc
     7441 gggaccggga taccaggcgc ccaccagtcc cgcgttgtgg ccggcgttgg cgtaatccat
     7501 gccgccggtg cccaacacct tgacacggct ggcgtcgata tcggcttgcg gcatcagccg
     7561 cgcgatccgt tccaaattct cctgcccgcc gggtaggaac aaggcgtcga tttggccgcc
     7621 ttcgctgtcc tgggcggcac gcaacaccgt gccgaccttg cgcatgggct cgagcatgcc
     7681 gttggccgag gccggatagg tctcgagcgc cacgatggtg ccgccggcgc gctcgacggc
     7741 gtcccggaag gcggtgccgg cgagcttgcc gaaggcgtcg tccggtatga gagcagcgaa
     7801 gcgacgcttg ccctggcgca ctgcgtagct gacgatgcgg ccaatttcgg gcgcgggctg
     7861 gaaactcaac agataaacgc cgcgtccggc cacctggcga tcgttggaaa aggcgaccac
     7921 gggcaccttt gcctggcggg caaacggcgc gacggcggcg acggatttgg caaacagcgg
     7981 accaacgatc aggctggcac catccttgat ggcatcgctc gccgccaccg ccgcaccttc
     8041 gggggtgcct ttgtcgtcct tgatcagcaa ctgcaggttt ggactgtcgc ggtcgaacag
     8101 cgcgagctca cccgactgtt tcagagcctt gccgatgatg ccgggttggc cttgcgccga
     8161 cagcgaggcc agcagtgcca ccttgaccgg ggcgcccttt ggcccggtct ccgatcccgg
     8221 cgtcgtggcg gaggtcgcga gcggtcccgg cgtattggcg gcgcagccca cgaggaggag
     8281 ggctgcggcc acgcgcgcga gggtggcgag cggacggtga cccacccggg ctgccgcggc
     8341 tgccagcgct ccccccatag gttgtcgtct cgcgcgcgtc gtctcaaccc ccggcctgcg
     8401 aatccctttc cgagcaaggc agagccagcg tcgtcgcgca acaaaaaaac gcgcaacggg
     8461 atggcgcttt gccgtccggc agcggcataa ctgccagtat tccaggtggc ccgaggaggg
     8521 accgtgacgc gacctgctca acctttgccc ggcgccggcg ccgcaacggc cgagcgtgct
     8581 ggcacgctgc tggcggctga gctagcgcag gctttgccgc ccggcctcta tattgtcgcc
     8641 acgccgatcg gcaatctggg cgatatcagt ctgagggcgc tggcgacgct ggcgcgggcc
     8701 gacctcatct actgcgagga cacgcgtcac agccgcaccc tgatggcgca cttcgccatc
     8761 gcccggccct tgcgcgccta tcacgagcac aatgccgagc gcgagcggcc acgcctcatc
     8821 ggcgagctca cggccggccg ctcggtcgcg ctgatcagcg atgccggcac gcccttgatc
     8881 tccgaccccg gctacaagct ggtgcgcgag gcgcttgccg ccggcatccg ggtaacgagc
     8941 ctgccgggcg cctcggcgct aacggcggcg ctcacctgct gcggtcttgc caccgatacc
     9001 gttctgttcg ccggctttct gccacccaag cagagtgcgc gccgcacgcg cttggcggag
     9061 cttgccggca cgccggcaac gctcgttcta ttcgaggcgc cctcgcgcct ggccgccagt
     9121 cttgccgaca tcggccaagc cttgccgcaa cgcccaatgg cggtcgcgcg cgagctgacc
     9181 aagctgcacg aggaggtgcg aacgggggac gcgcacgagc tggcgcaatg ggcgtcccaa
     9241 tcggcgccac gcggcgaaat ggtgatcgtc gttggcccac ccgccaccgc gcccagcgtg
     9301 gccgacaccg cgattgccgc ccacttgcgc gacctgctca aggacatgag cgtgcgcgac
     9361 gccgccaggg cgacggcgga ggccctcaat gtctccaaag cgcgtgctta tgagctggcg
     9421 ctggcactca aaaaagacga ggacaactga gcagtgccat ctcgacgccg ccgcatggag
     9481 cctggccaag cgacagccgc cggcgcgtgc gggcatgcaa ttccggttga acttgccaac
     9541 ggaaagggct tgcatgccgt ctcgcctgcg gccgtgcggc cgggagtgtg gatccaatgc
     9601 ccctcaaagt tgcctgccag atggacccga tcgaccgcat cgacattcgc ggcgactcga
     9661 cctttgcctt gttgctggag gctcagcagc gcggccacca gctcttctac tacaccccgc
     9721 cgcacttgag cctgctggcc gggcgcctga tcgcgcgggg cacaaccctc aatgtcgagg
     9781 atcgggtggg caaccactac cgggtgggcg acgagcgcga ggaggacctg agccggatgg
     9841 acgtggtgct cctgcgccag gatccaccct tcgacatgtc ctacattacc acgacgcacc
     9901 tgctcgagcg catccatccc aagaccctgg tggtcaacga cccagcccac gtgcgcaatg
     9961 cacccgagaa ggtgttcgtg ctcgactttc tcgacttgat gccgccgacg ctggtcacgc
    10021 gccacctcga acaggtgcgc gcctttcgcg ccgtgcacaa ggacatcatc ctgaagcccc
    10081 tctacggcaa tggcggggct tccgtgttcc gcctgactga ggacgacacc aacctcaatt
    10141 ccctggtcga gctgttccag accgtgttcc gggaaccgtt catggtgcaa cagtatcggc
    10201 cggaggtacg cgccggcgac aagcgcatca tccttgtcga tggcgatgtg gtgggcgcca
    10261 ttaaccgggt tccggccgcg gacgagacgc gttccaacat gcacgtgggt ggggtcgcca
    10321 cggccacgga gctgacgcag cgcgacctcg agatctgccg tcggcttggc ccggagctca
    10381 agcgccgcgg cctcctcttc accggcatcg acgtgatcgg tccctatctg acggaaatca
    10441 atgtcacctc gcccaccggc atccggcagg tgaaggcctt cggcggcccc gacattgcgg
    10501 ccctgatctg ggatgctatc gacgccaagg tgcgccgcta gcgggcgccg ttttgcgccg
    10561 cctcttcgac gcatgaggcc aggggcgcgc gaaagtcctc cgcgcgcagc cgcgcggcgt
    10621 ttggcccgcg cgacgccagg ctcaatgcgg gccgctggat aagcgccaac ctcgggcatt
    10681 gggccttggc tttggcgaca ggcctggcgc ggggcttaca tggtggcgcc gatctgccag
    10741 ggtacgaatt cgttatcgcc gtagcccaac agctcgcttt tggttttttc gccggaggcc
    10801 acgcgtagga tggcgtcgaa gatctcctga cccttggcgt cgatcgagac gccgtccagg
    10861 atatcgccgc agtcgatgtc catgtcgtcc agcatgcgcc gatagacgtc gtgattggta
    10921 gccagcttga tcgacggcgt cggcttgcag ccgtacgccg agccgcggcc cgttgtgaag
    10981 caaagaagat tgcagccacc ggctacctga cccgtagcgg ccacgggatc gtagccgggc
    11041 gtgtccatat aaacgaagcc cttggcggtg accggctcgg cgtagcgata gacctcgttg
    11101 agcgtggtgg tgccaccctt cgccgccgcg cctagcgatt tctccaaaat cgtcgtgagc
    11161 ccgccggcct tgttgccggg gcttggatta ttgtccatgg agccatcgtt cctggcgcaa
    11221 tagtcctgcc accaacggat gatctcgacc agcttctcgc cgacctggcg attacgcgcg
    11281 cgccgggtca ggaggtgttc ggcgccatag atttccgggg tctccgacag caccgcggtg
    11341 ccaccatggc gcaccagcag gtcggcggcg gcaccgagcg cgggattggc ggtgatgccc
    11401 gagtagccgt ccgagccgcc gcactggagc gccagcgtga tctcgctggc cggcacggtt
    11461 tcgcgtcgga ccgcgttggc acgcggcagc atctccttga tgcgtgccac gccagcctcg
    11521 atggtcttct tggtgcctcc ggtatcctgg atcgtcatgg tacggaagtt gtcgccctcc
    11581 accaggccat atcgttcctt gaggcgcgcg atctggaaca cctcgcagcc gagcccgacg
    11641 agcagcgtgg cggcgaggtt aggatggccg gcgtaacccc actgtgtgcg ctccaagacg
    11701 tcgtaaccct cgcccttggc agcgagccca cagccggtcc cgtgcacgaa agagacgaca
    11761 ccgtccacgt tgggataggc ctcgaggaga cccgagcggc ttaccgcctc ggccatgaag
    11821 cgggccaccg aggcggagca gttcacgctc gtcaacacgc ctatatagtt gcgcgtgccg
    11881 atcttgccgc tgctgcgtcg gaagccggca aaggtcgcgc gcagctcggc cggcaacagg
    11941 acctcctcgc gggcatcctg ggcgaaggca taatcgcgct cgaactcggc gaacgaacag
    12001 ttctgcgtgt ggacgtggct acccggggcg atgtcctcgc tggcaaagcc gatgatctgt
    12061 ccaaatttga ggacgggctg tcccttggcg atcggctgcg ccgccatctt gtgcccgcgt
    12121 agaatgcgcg cacgcgccgt caccccgtcg atcgtagagc ctcgctcgac gggatcgacg
    12181 gcgaggagga tattgtcgcg cggattgagc cgcagcgtgc ggggggtcga ttgttgcggg
    12241 ctatcctgca tggctcgcgc tcccgtggtt ggccgcatca tgcaccattt ttgcgcttga
    12301 tgcgatccga tgcgctaagc tgcagccaac gggctgccgc cgcgagcagg ccccgtggaa
    12361 cgccgttgtc agcttcgctc ttcggaggac tagtccccgt gaacttagca tccctgctca
    12421 aggcgcgcgc catcgatgcc aaacccattc gtgtcggcca gatcggcgcc ggaaaattcg
    12481 gcaccatgtt cttgtcgcag gtgcggctga cgccaggcat gcatttggca ggcctggccg
    12541 acctcgtgcc ccagcgcgcc aaggatcggc tgagcgaggt cggctggccg gcggctcagc
    12601 tcgacgtcgc cagcctcaac gatgccatca agaccggccg gacctttttg accgacgatg
    12661 ccatggccat cgtagcgcac cccgagatcg aggtggtgat cgaggccacc ggcgaccccg
    12721 cgaccggcat tcgcctgtgc caggctgcca ttcagcacgg caagcatgtc gtcatggtca
    12781 atgtcgaggc cgacgcgctc gccgggccgc tgctggcacg ccgggcacgc gaggcgggcg
    12841 tcgtctattc gctggcctgg ggcgatcagc cggcgctcat ctgcgagcac gtcgactggg
    12901 cacgcacctg cggtttcgag gtgatcgccg ccggcaaggg cacgcgttac caccccagct
    12961 accatcagtc gacgccggac acggtctgga acatcctcga cgcctacctg aaaatcaccg
    13021 atcgcagcca catcaacccg aaaatgttca actccttcat cgacggcacc aagtcgggaa
    13081 tcgagatgac tgcggtgtgc aacgccaccg gcctgctgcc gcagacgaac gggctcggct
    13141 ttccgccctc gtcccggttc gatcttgccg aagtctgcaa gccccgcagc gacggcggca
    13201 gcttggag
//
LOCUS       DASTMT010000487         3096 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_936072, whole genome shotgun sequence.
ACCESSION   DASTMT010000487 DASTMT010000000
VERSION     DASTMT010000487.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3096
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_936072"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            137..670
                     /locus_tag="VFR00_11205"
     CDS             137..670
                     /locus_tag="VFR00_11205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0159877.1"
                     /translation="MPRVNAAESFLWRAAPQPGRTRLLGALAIALSCGALGVLAGRWS
                     IERVPSEPGTRTAALIEAVAKETQAKLAVAARAPQMQAAPPAGISAAVAPAAPSAPTA
                     SRANSAVTPEPPQEPSPSEPAAGEATPLSRSEHAEQQQPAAVRPAARELRRPQPREPH
                     ETTAPDYRALRDYVLSR"
     gene            complement(807..1028)
                     /locus_tag="VFR00_11210"
     CDS             complement(807..1028)
                     /locus_tag="VFR00_11210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009797575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0159878.1"
                     /translation="MIKQLLLAGAAIGVMSSTALAAEYYIVQEKGTKKCKVVETRPSE
                     TTWVQVGPMAFKTRDEADKQLAVVCKEKR"
     gene            complement(1224..2213)
                     /locus_tag="VFR00_11215"
     CDS             complement(1224..2213)
                     /locus_tag="VFR00_11215"
                     /inference="COORDINATES: protein motif:HMM:NF019843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU0159879.1"
                     /translation="MRIFSGAAVALWLAGSVGGAAAGDAVKYKSVDLAFEQGLGAYKS
                     GYYEMAIPAFEQTIAEGSEFDRFFAEFYLARIYSGAGVLTNHAKAYRLYQTLANENAD
                     ADPEDGRRAPFVAKALTALAGYLQRGLPDIGVKADPQRAVDYLHHAATFFGDKDAQFE
                     LAKIYLNGTGSADDAKRGMHYLSVLTEEGHPGAQAFLADLLWRGVHVKKDERRALALI
                     TMAVENAPAHERIWIEDIYQNIFCATSQKTRKQADGIAAIWRKMFAPNPEAPPQMALG
                     AGDLQPRRACGNGEMLDLQSTPLVAGKEVATSPPAAGSAPPKAIQGNPIGFGR"
     gene            complement(2200..>3096)
                     /locus_tag="VFR00_11220"
     CDS             complement(2200..>3096)
                     /locus_tag="VFR00_11220"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEU0159880.1"
                     /translation="STNAGLHLPAMAHECGIEFDLMEVAAIFKRTPYIADLKPGGKYV
                     AKDVYDIGGVPQIMRALLDGGYLHEDCITVTGKTIKQNLETVKFNSDQKVVYPVSNPI
                     SPTGGVVGLQGSLAPDGAIVKVAGMSKLQFRGPARVFDCEEDCFAAVEKRQYSEGDIL
                     IIRYEGPKGGPGMREMLSTTAALYGQGVGDKVALITDGRFSGATRGFCIGHVGPEAAA
                     GGPIALVRDGDLIAIDAVAGRLDLEVSEEELARRRKSWTAPANAYQSGVLRKYADQVG
                     PARKGAVTHAGGRAEVVCYADI"
BASE COUNT          499 a         1065 c          987 g          545 t
ORIGIN      
        1 ggcccagagc aaccgctgac ccggacgcat ggttttgccc ctttggcgga caggatcgcc
       61 cggcagcgcg gcaccgacgt catcgtcatc atgcggcgca ccacgccgcc ctacgactat
      121 aaatacgtgc gcggttatgc ctcgcgttaa cgcagcggag agtttcctgt ggcgcgcggc
      181 gccgcagcca gggcgcacac gcctgctggg cgcgctcgcc atcgcgctca gctgcggtgc
      241 cctcggcgtg ctggccgggc gctggtcgat cgagcgcgtt ccctccgagc ccggcacgcg
      301 cacggcagcg ctgatcgagg ccgtcgccaa ggagacgcag gccaaattgg cggttgccgc
      361 ccgcgcgccg cagatgcagg ccgccccgcc agcgggcatc tctgcagccg ttgcccccgc
      421 ggcgcccagc gctcccaccg caagccgcgc caacagcgcc gtgacccccg agccgcccca
      481 ggagccctca ccgtccgagc cggccgccgg cgaggcgacc ccgctttccc gcagcgagca
      541 cgccgagcag cagcaaccgg ctgccgtgag gccggccgcg cgcgagctgc gccggccgca
      601 gccgcgtgag ccgcatgaga ccaccgcacc cgattaccgg gccttgcgcg actatgtctt
      661 gagccgctga gggcgctgca ggcggcggac gcggcagcac gccggcgtcg cacggcacgc
      721 caaaattacc ttgcctttac ggtcaatgcg gcaatggccg agctgggccc ggccattgct
      781 ttacgtcgcg ggtcctggtg gacgcgtcag cgcttctctt tgcacacgac ggccagctgc
      841 ttgtcggcct catcgcgcgt cttgaaggcc atcggcccga cctgcaccca ggtcgtttcg
      901 ctcgggcggg tttccaccac cttgcacttc ttggtgccct tctcctgcac gatataatac
      961 tcggctgcca acgcggtcga gctcatgacg ccgatggcgg cgcccgccag caaaagctgc
     1021 ttgatcatgg ttcagctcca gtttgtcatt tcacatggct ggctaacgcg cgtgccaagc
     1081 cttgcgttcc gagcgcagcg gccccgcgct tggcgaggag cacggctgca ggggacaccc
     1141 cgtgcgcagc aaaggagagg gttgggcgcg aacgcggcgg cgctgggccg ctcgcggctg
     1201 tgcgctaagc gttgccgcgc aagctagcgg ccgaaaccga tcggattgcc ctggatggct
     1261 ttgggcggcg cgcttcccgc cgcgggcggg ctggtcgcca cctccttgcc ggcaacgagg
     1321 ggcgtgcttt gcagatcgag catctcgcca ttgccgcagg cacgccgggg ctgcaagtcg
     1381 ccggcaccca gcgccatctg tggcggcgct tccgggttgg gggcgaacat tttgcgccag
     1441 atcgcggcga tgccgtcggc ctgtttgcgc gtcttctggg aggtggcaca gaagatgttt
     1501 tggtagatgt cctcgatcca gatgcgctcg tgcgcgggcg cgttctcgac cgccatggtg
     1561 atcagcgcca gcgcccgccg ctcgtccttt ttgacatgca ccccgcgcca gaggaggtcg
     1621 gcgagaaagg cttgcgcgcc gggatgaccc tcctcggtca ggaccgacag atagtgcatg
     1681 ccgcgtttgg catcgtcggc actcccggtc ccgttcagat agatcttggc cagctcgaat
     1741 tgtgcatcct tgtcgccgaa gaacgtcgcg gcatggtgca aatagtcgac cgcgcgctgg
     1801 ggatcggctt taacaccgat atcgggaagt ccgcgctgca ggtagccggc gagcgcggtg
     1861 agcgccttgg caacgaacgg cgcccggcgt ccgtcttcgg gatcggcatc cgcattttcg
     1921 ttggcgagcg tctggtagag ccgataggcc ttggcgtggt tcgtcaaaac accagccccg
     1981 gaatagatgc gggccagata aaactcggcg aagaaccggt cgaattcgct accctcagca
     2041 atggtctgct cgaacgccgg gatcgccatt tcgtagtagc cggacttgta ggcgccgagc
     2101 ccctgctcga aggcgaggtc gaccgacttg tacttcaccg catcgcccgc tgctgcgccg
     2161 cctacggacc ccgcaagcca gagcgccacc gccgctccgc taaatatccg cataacagac
     2221 aacttccgcc ctcccacccg catgggtcac cgcgcccttg cgggccggtc cgacttgatc
     2281 tgcatatttc ctcaagacgc cgctttgata ggcgttggct ggcgccgtcc agctcttgcg
     2341 ccggcgcgcc aattcctcct cggacacctc cagatcgaga cgaccggcaa ccgcgtcgat
     2401 ggcaatgagg tcaccgtcgc gcaccagcgc gatcggtccg cccgccgcag cttccggtcc
     2461 gacgtgaccg atgcaaaaac cacgcgtggc gccggagaac ctgccatcgg tgatcaaggc
     2521 caccttgtcg ccgaccccct ggccataaag cgccgccgtg gtcgacaaca tctcgcgcat
     2581 gccggggccg cccttggggc cctcgtagcg gatgatcaga atgtcgcctt cgctgtattg
     2641 gcgcttttcg acggcggcaa aacagtcctc ttcgcagtcg aacacgcgcg ccgggccgcg
     2701 gaactggagc ttgctcatgc ctgccacttt cacgatagcg ccatccggcg ccagcgagcc
     2761 ctgcagcccc accactccgc ccgtaggtga tatggggttc gaaaccgggt agacaacctt
     2821 ttgatcggaa ttgaatttca cggtttcgag attctgcttg atggtcttgc ccgtgacggt
     2881 gatgcaatcc tcgtgcaaat agccgccgtc gagcagcgcg cgcatgatct gcggcacgcc
     2941 gccgatgtca taaacatcct tggccacgta tttgccgccc ggtttgaggt cggcgatgta
     3001 gggcgtgcgc ttgaagatgg ccgccacttc catgagatcg aattcgatgc cgcattcgtg
     3061 cgccatggcg ggcaggtgca agccggcgtt ggtgga
//
LOCUS       DASTMT010000488         3115 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_5066162, whole genome shotgun sequence.
ACCESSION   DASTMT010000488 DASTMT010000000
VERSION     DASTMT010000488.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3115
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5066162"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..215)
                     /locus_tag="VFR00_11225"
     CDS             complement(<1..215)
                     /locus_tag="VFR00_11225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012563217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEU0159881.1"
                     /translation="MRPLEGLRVVDLTRVLSGPYCTMQLGDLGAEVIKVEQPGKGDDT
                     RAFAPPYQGDEAAYFLSVNRNKKSITL"
     gene            complement(388..618)
                     /locus_tag="VFR00_11230"
     CDS             complement(388..618)
                     /locus_tag="VFR00_11230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2093 domain-containing protein"
                     /protein_id="HEU0159882.1"
                     /translation="MNRFEKLLGLKGEAKLRYLDGEFQVLSPGDFVRCAVTGEPIMLP
                     DLRYWSVELQEAYANAEISFERFRALRRGPKP"
     gene            complement(786..2384)
                     /gene="xseA"
                     /locus_tag="VFR00_11235"
     CDS             complement(786..2384)
                     /gene="xseA"
                     /locus_tag="VFR00_11235"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009758965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEU0159883.1"
                     /translation="MAELVRASRQGGNIAEFTVSELSNAIRRTLEGNYGYVRLRGEIS
                     GFRGVHGSGHCYFTLKDDRARIDAVIWRTTLSTLRIRPAEGLEVIAVGRVTSYPGKST
                     YQIVIDALEPAGIGALMALLEERKKKLAAEGLFDAARKKPLPFLPRAIGIITSPSGAV
                     IRDMLHGFDERFPVRVLVWPVRVQGPTCAAEVAAAIRGFNALRPGAAVPQPDLLIVAR
                     GGGSLEDLWGFNEEMVVRAAAESHIPLISAVGHETDTTLIDLAADARAPTPTKAAEWA
                     VPRYGQLTQQVDELVRRLQAAARRRLDDLKTHLRAAGRGLPRRQDLLALPRQRFDGVE
                     ARLARALIANTRAHAVHLTRTASRLRLRLLDVGLARARDRLEALGRRTSAALVRNAGP
                     KRARLERVAGRLSPNLVLARLGRAGERLEVFAGRLCRARRADITARRGRLDAAAKLLA
                     SLSYQAVLQRGYALVRGANGRSIRSVGAVSAGERLAIELADGHLAATAEGSAAALAPP
                     APRLPPRPKARAKARGGEGQGSLF"
     gene            2493..>3115
                     /locus_tag="VFR00_11240"
     CDS             2493..>3115
                     /locus_tag="VFR00_11240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019856953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEU0159884.1"
                     /translation="MNVLLIGSGGREHALAWALSASPLLRKLYCAPGNAGIAALAECC
                     DLDVRDHGAVVRFCRERRIALVVIGPEAPLVAGLADDLTAGGIKVFGPSKAAARLEGS
                     KGFTKDLCRDFNIPTASYRRFAEREAALAYVETHSLPVVVKLDGLAAGKGVSVAETRT
                     AAREAVQACFASGGDVVIEEYLAGEEASFFALVDGTHVLPLATAQDH"
BASE COUNT          510 a         1026 c         1086 g          493 t
ORIGIN      
        1 tcgagggtga tgctcttctt gttgcggttg acggacaaga aataggccgc ctcgtcgccc
       61 tgatagggcg gggcgaaggc gcgcgtatcg tcgcccttgc cgggctgctc caccttgatc
      121 acctcggcgc cgagatcccc gagctgcatg gtgcagtagg gcccggatag gacccgcgtg
      181 agatcgacga cacgaagccc ttcgagagga cgcatggact acctcgcagg atcacaggga
      241 gcacagccgg cgggcgcggc cagccaagac ggaagggcgg caggcgccac cacccaatag
      301 cggccagcgg cgcgacggca tagagcgcgg tggcatgcaa gcatcgattg gacttgcctc
      361 gaaccccgtt ggcgaccgta gcagccttta cggcttgggg ccccgccgca gggctcggaa
      421 gcgctcgaaa gaaatttccg catttgcgta agcttcttgc agctccacgc tccaatagcg
      481 cagatccggc agcataatcg gctcgcccgt gaccgcacag cgcacgaagt cgccgggact
      541 caagacctgg aactcgccgt cgagatagcg cagcttggcc tcgcccttca gtccgagcag
      601 tttttcgaat cgattcattg ctgtgcgatc attgctgtgg aaatctctta gacattcagg
      661 agcatacggg taggcgagca tacaggtcgc ggctggccta ggccagcctg atagcacggg
      721 ggagggcaat tcccacacgg cgttgcaggt gtttgcccca ggacctcagg gcaggccagc
      781 gcaggtcaaa agagcgaacc ctggccctcg ccacctcgcg ccttggcccg ggccttcggc
      841 ctcggcggca ggcgcggcgc cggcggagcc agcgccgcgg cgctcccctc ggccgtagcc
      901 gccagatgcc catcggcgag ttcgatagcg aggcgttcgc cggcactcac ggcgcccacc
      961 gagcgtatgc tgcgcccgtt ggcgccgcgc acaagggcgt agccgcgctg caagacagct
     1021 tgatagctga gcgaggccag tagcttggct gctgcatcga gccgaccgcg tcgagcggtg
     1081 atatcggccc ggcgggcccg gcaaaggcgg cccgcgaaga cctcgagccg ctccccggca
     1141 cgcccgagcc gcgccagcac gaggtttggc gacaggcggc cggcaacacg ctcgagcctg
     1201 gcgcgcttgg gtccggcatt gcgcaccagt gccgcggagg tgcgccgccc cagcgcctcg
     1261 agccggtcgc gcgcgcgcgc caggccaacg tccaagagtc gcaggcgcaa cctcgatgcg
     1321 gtccgcgtca gatgtacggc gtgcgcgcgg gtgttggcga tgagcgcgcg cgccagtcgc
     1381 gcttccactc cgtcgaagcg ctggcgcggt aacgccagca ggtcctggcg ccgcggcaaa
     1441 ccgcgcccgg ccgcgcgcag atgcgttttg aggtcatcga gccggcgtcg cgccgcggcc
     1501 tgcaggcggc gaaccaactc gtcgacctgc tgcgtcaatt gcccatagcg cggcacggcc
     1561 cattcggcgg ctttggtcgg tgtcggcgcg cgggcgtcgg cggcaagatc gatgagcgtg
     1621 gtatcggtct cgtgtccaac cgccgaaatc aaggggatgt ggctttcggc ggcggcacgc
     1681 accaccattt cttcgttgaa gccccacaag tcttcgaggc tgccgccgcc gcgtgccacg
     1741 atcaacagat cgggctgcgg caccgcagcg cctgggcgca gcgcattgaa gccccggatg
     1801 gcggcggcga cttccgcggc gcaggtcggg ccttgcacgc gcacgggcca aaccaagacg
     1861 cgcaccggaa agcgctcgtc gaagccgtgc aacatgtcgc gaatgaccgc gccgctcggg
     1921 gaggtgatga tgccgatggc acgcggcagg aacggcaggg gtttcttgcg cgccgcgtcg
     1981 aacaggccct ctgccgcgag cttcttcttg cgctcctcca aaagcgccat caaggcgccg
     2041 atgcccgccg gttccaaggc atcgatgacg atctggtagg tggacttgcc cggataggag
     2101 gtgacgcgtc caacggcgat gacctcaagc ccctcggcgg gacgaatccg cagcgtgctc
     2161 agggtcgtgc gccagatcac ggcatcgatg cgggcgcgat cgtccttgag cgtgaaatag
     2221 cagtgccccg agccgtgcac gccgcggaag ccggagatct ccccgcgcag gcgcacgtag
     2281 ccgtagttgc cttccagcgt gcgccggatg gcattggaga gctccgagac cgtaaactcg
     2341 gcaatgttgc cgccctggcg ggaggcgcgg actagctcgg ccacgcgtcc cccctggctt
     2401 tgtcgagcgg ggctgcaagt gctagaggag ggcgcgaagc cgaggcctcg tccgttgacg
     2461 aggctggcgg agcttgcgtg gggtcttggc tgatgaacgt tctcctgatc ggctcgggtg
     2521 gccgcgagca tgcgctggcc tgggcgcttt ctgcaagtcc gctgctgcgc aagctttatt
     2581 gtgcgccggg gaacgccggc atcgctgccc tggcggagtg ctgcgacctc gatgtccgcg
     2641 atcacggcgc cgtcgtgcgc ttttgtcggg agcggcgcat tgctttggtc gtgatcgggc
     2701 cggaggcccc cctggtagca gggcttgccg acgatctgac ggcggggggc atcaaagtgt
     2761 tcgggccctc gaaggccgcc gcacggctgg agggctccaa gggcttcacc aaagatttgt
     2821 gccgcgactt taacattcca accgccagct accgccgctt tgccgagcgc gaggcggcgc
     2881 tggcctacgt tgagacccat tccctgccgg tggtcgtcaa gctcgacggc cttgccgccg
     2941 gcaagggcgt cagcgtggcg gagacgcgca cggccgcccg ggaggcggtg caagcctgtt
     3001 tcgccagcgg gggcgacgtc gtgatcgagg aatacctcgc gggtgaggag gcaagcttct
     3061 ttgccctggt cgacgggaca catgtgctgc cgctcgccac ggcgcaggac cacaa
//
LOCUS       DASTMT010000489         4926 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_611942, whole genome shotgun sequence.
ACCESSION   DASTMT010000489 DASTMT010000000
VERSION     DASTMT010000489.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4926)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4926)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4926
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_611942"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(32..1444)
                     /locus_tag="VFR00_11245"
     CDS             complement(32..1444)
                     /locus_tag="VFR00_11245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019905404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0159885.1"
                     /translation="MAIPIVPLVVATALFMENTDSTILATALPTIARDLGLDPISLKL
                     AVTSYLVSLAVFIPISGWVADRVGARTTFRLALGLFMLASIGCAYSSSLAGFVFWRAV
                     QGMGGAMMVPVGRMVVIRTVAKNELVRALSFIAMPALIGPMLGPPIGGLIVTYWDWRW
                     IFFVNIPIGILGIILASLYVPNIKQETAPLDVGGFVLCALGLGGLILGAAMTGRHIAP
                     VEFAVGSMLIGAVMLMGYVRHARTTERPLLDLRLMHFKTFEAGILGGTFFRFGVGASA
                     FLLPLMLQLGFGLDPLRSGLTTFAGAVGALLVKPLARSFLFRFGFRRLLIVNGLLASL
                     TLMAFALFKPTTPHVIILGVLLLGGFLRSLEFTSLSAITYAELETRQISAATGMASVA
                     QQVSISLGVATGAMVLEVSAWAAGRGAPAVADFAAAFVVVGLVSAMTSLLMLRLPVGA
                     GDEIAGRVLPPADPPRVRPA"
     gene            complement(1580..2599)
                     /locus_tag="VFR00_11250"
     CDS             complement(1580..2599)
                     /locus_tag="VFR00_11250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mitochondrial fission ELM1 family protein"
                     /protein_id="HEU0159886.1"
                     /translation="MTARAATVGRRGWIISDGKRGNEVQTGGVFEALGLLSEIKLVEP
                     RGLWRLLSPWGGVSPSERFGGPQSPFRPPWPDFAIAVGRLTTPYIRALKRKAGLATYT
                     IILQDPKVSAAAADLFWVPEHDVRRGPNVVTTLTAPHGFSERTLADLRQHLPPALAAL
                     PSPRVALSLGGPNGDYRYGAEGMQHLESALRSLAALGAGLMITPSRRTPAAVLASVQA
                     AVEGLAAWVWDGQGDNPYPHFLAHADAFIAPADSINMTGEPCATGRPVFVFNPGGGSP
                     KFDRFHRALERYGATRPLPRHFDRLPTWSYRPLNSAETIAEEIARRWGKRKQMLGPTL
                     GRRLP"
     gene            2710..3204
                     /locus_tag="VFR00_11255"
     CDS             2710..3204
                     /locus_tag="VFR00_11255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEU0159887.1"
                     /translation="MRVRLDAIDWRILKELQANGRVSNVELARKVGISPPPCLRRVRA
                     LEEAGMIAGYFAVLDEKSVGFEVIAFAMVGLHSQSEPDLRAFENRVLAWSLVRECYML
                     SGESDFQLKCVAPDLTAFQDFLIEELTAAPNVASVKTTLVIRRVKFEPGVPVAQARTH
                     QEGV"
     gene            3339..3887
                     /locus_tag="VFR00_11260"
     CDS             3339..3887
                     /locus_tag="VFR00_11260"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEU0159888.1"
                     /translation="MVEFLPRLRRFAVALTGDLDQGDDLVQETCLRALSRVDQWQPGT
                     RLDSWMYRIAQNLWLDRTRARKHRSELSDPEIVEAVPGQDGRSVVEGRLTLEAVLEAM
                     TDLSPEQQVLVALVCIDGASYKEAAEITGTPIGTVMSRLARARRELHERLEPALPAER
                     PRPAPDLPASTRLTSTKAGTRL"
     gene            3884..4663
                     /locus_tag="VFR00_11265"
     CDS             3884..4663
                     /locus_tag="VFR00_11265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0159889.1"
                     /translation="MTHISDADLMAYADGVLPRHLRPLVRQALLRDPALLEKLECYII
                     TNRGLAAPFDRLPPIPDRLLRLLAEGQPKAAGERWWLFSLRSIEPKWAEQWRLPAWSL
                     AGLAALVAATLLAWQLNTASPLRPQLADAGAQGLASSAQFQRALEASESNLSTHLASV
                     KPTGTFLSKEQAWCRQYEVTRADDTRLAGIACRDHHGAWQVKAQAVLAAAAQTSGYAP
                     AGEGKAGAHGLESALGKLMQDVVLLPADEKRVLAGGWQPPR"
BASE COUNT          889 a         1661 c         1660 g          716 t
ORIGIN      
        1 cgatctggcg accccgcacg cgcccagcgg cctaggccgg ccgcacgcgc ggcgggtcgg
       61 cgggtggcag gacgcggccg gcgatttcgt cgccggcacc gacgggcagc cgcagcatca
      121 gcagcgacgt catggccgag acgagaccga ccacgacgaa ggcggcggca aaatcggcga
      181 ccgccggcgc cccacgcccc gccgcccacg ccgacacctc gagcaccatg gcgccggttg
      241 ccacgcccaa actgatcgac acctgctggg cgacgctggc catcccggtc gcagcgctga
      301 tctgccgggt ttcgagctcg gcgtaggtga tggccgagag actggtgaac tccagcgagc
      361 gcaaaaatcc ccccagcagc aagacgccca gaataatgac gtgcggggtg gtcggcttga
      421 agagcgcgaa ggccatcagc gtcagggagg ccagcaggcc attgacaatc aagagccgcc
      481 ggaagccaaa tctgaaaagg aaactgcgcg ccagcggctt caccagcagc gccccgaccg
      541 ccccggcaaa ggtcgtcagg ccggagcgca agggatcgag cccgaagccc aactgcagca
      601 tcagcggcag caagaaggcg ctggcgccca cgccgaagcg gaagaaagtg ccgccgagga
      661 tgcctgcctc gaaagtcttg aagtgcatca ggcgcaaatc gagcagcggc cgctccgtcg
      721 tccgggcatg gcgcacatag cccatcagca tcaccgcgcc gatcagcatg gagccgaccg
      781 cgaactcgac cggggcgatg tggcgaccgg tcatggccgc gcccaggatg aggccgccaa
      841 ggccgagggc gcaaagcaca aagccgccga cgtccagcgg cgcggtttcc tgcttgatat
      901 tgggcacgta gagcgaggcc aggatgatgc cgaggatgcc gattggaatg ttgacgaaaa
      961 atatccaccg ccaatcccaa taggtgacga tcaagccgcc gatcggcggc ccgagcatgg
     1021 gaccgatcag cgccggcatg gcgatgaagg aaagcgcccg caccaactcg ttcttggcga
     1081 ccgtgcggat cacgaccatg cgtccgaccg gcaccatcat ggcgccgccc atgccctgca
     1141 cggcgcgcca gaacacgaag ccggccagcg aactcgagta ggcgcagcca atcgaggcca
     1201 gcatgaagag gccgagcgcc aggcgaaacg tggtgcgggc gccgacgcga tcggccaccc
     1261 agccgctgat cggaatgaac acggccaggc tgaccaggta ggaggtgacc gcgagcttga
     1321 gcgaaattgg atcgagcccc aagtcgcggg cgatggtggg aagggcggtg gccagaatgg
     1381 tggaatccgt gttctccatg aagagcgcgg tggcgaccac caaaggcaca atgggaatgg
     1441 ccatcgctca ggccttgcga ggggctgcgg ggatggcggg caaccacccg ccggggggag
     1501 gagaactggc cggttgcata tccggccaaa cgcggcggcc gtcaatagcg ccaagcggcg
     1561 ccaggcgccc tggcccgcgt cacggcagcc tgcgccccag cgtcgggccc aacatctgct
     1621 tgcgcttgcc ccagcgccgc gcgatctcct cggcgatcgt ctcggccgag ttgagcggcc
     1681 gatagctcca ggtcggcaag cgatcgaaat gtcgaggcag cgggcgcgtc gcgccgtagc
     1741 gttccagcgc ccgatgaaag cgatcgaact tcggcgagcc gccgccggga ttgaagacga
     1801 aaacgggccg cccggtcgcg cacggctccc cggtcatgtt gatggaatcg gcgggcgcga
     1861 tgaaggcgtc cgcatgcgcc aggaaatggg gatagggatt gtcgccctgc ccgtcccaga
     1921 cccaggcggc gaggccctcg accgccgcct ggacgctggc gagaacggcg gcgggagtgc
     1981 gccgcgacgg cgtgatcatc agccccgccc ccaaggccgc cagcgagcgc agcgcacttt
     2041 caaggtgctg catgccctcc gcgccgtagc gatagtcgcc attgggtccg ccgagggaaa
     2101 gcgcaacccg cgggctgggg agtgctgcca gcgccggggg cagatgctgc ctcagatccg
     2161 caagcgtgcg ctcggagaag ccgtgtggcg cggtcaaggt cgtcaccaca ttggggccgc
     2221 ggcgcacgtc atgctcgggg acccagaata gatcggccgc cgcggcgctc accttcgggt
     2281 cctgcaggat gatggtgtag gtcgccaacc cagccttgcg cttgagcgcg cgaatgtagg
     2341 gggtcgtcag gcgtcccacc gcaatggcaa agtccggcca cggcggtcga aaggggcttt
     2401 gcgggccgcc gaaccgctcg gacgggctga cacctcccca aggcgagagc aaccgccaca
     2461 agccgcgagg ctcgaccagc ttgatctcgc tgaggagacc caaggcctcg aacacgccgc
     2521 cagtttgcac ctcgttgccg cgcttgccgt cgctgatgat ccagccgcgc cgtcccacgg
     2581 tcgccgcccg cgcggtcatg ccaggcctcc gcaaatttgc cacggcccgc cgcgcgcgcg
     2641 ggaaaacgac attgcgccgg cggctgcttt ggccgaacac ttcgcccaat tgcttggagc
     2701 cccgatgcca tgcgtgtccg cctcgacgcc atcgattggc gcattctcaa ggagctgcaa
     2761 gccaacgggc gggtcagcaa cgtcgaactg gcgcgcaagg tcggcatttc cccgccgccc
     2821 tgcctgcgcc gcgtgcgggc gctggaggaa gccggcatga ttgccggcta tttcgccgtc
     2881 ctcgacgaga agagcgtcgg ctttgaagtg atcgccttcg ccatggtggg tctgcatagc
     2941 caatccgagc ccgatctgcg cgcctttgag aaccgcgtcc tggcgtggtc gctggtgcgc
     3001 gagtgttaca tgctgtccgg tgagagcgat ttccagctca agtgcgtagc cccagacctg
     3061 acggccttcc aggatttcct gatcgaggag ctgaccgcgg cgcccaacgt ggccagcgtc
     3121 aaaaccacgc tggtgatccg ccgggtcaag tttgagcccg gcgtgcccgt ggcccaggcg
     3181 cgcacgcacc aggagggcgt ctgaccccgg acctcgagtg gtttatgcca cacctcccga
     3241 gttgtttttc ccacttacga gaataaatgc cgccgtcagg cgttagatcc ccgacccgcg
     3301 gggagacagc gcgcgtgacg gacgagttcc gcacccgaat ggtcgagttc cttccgagac
     3361 ttaggcggtt tgccgttgct ctcacggggg acttggacca aggcgacgat cttgttcaag
     3421 agacatgctt gcgtgcgttg tcgcgcgtgg atcagtggca gcccggtacc agattagaca
     3481 gttggatgta tcgcatcgcg caaaatctgt ggctggaccg cacgcgggcc cgcaagcatc
     3541 gttcggagct gtcggacccc gagatcgtcg aagcggtccc tggtcaggat ggccgcagcg
     3601 tcgtggaagg ccgcctgacg ctggaagccg tcctcgaggc catgaccgac ctttccccgg
     3661 aacagcaggt cctggtggcg ttggtttgca tcgacggggc ctcctacaag gaggccgccg
     3721 aaatcacggg tacgcccatt ggcacggtga tgagccgcct ggcccgcgct cgccgtgagt
     3781 tgcatgagcg cctcgagccc gcactccccg cagagcggcc ccgtccggcg ccggatctgc
     3841 ccgccagcac acgtcttacc tcaacaaaag cagggacgcg gctatgaccc atatctcgga
     3901 tgccgatctc atggcctatg ccgacggcgt actgccgcgc catttgcgcc cgttggtgcg
     3961 ccaagccttg ctgcgggatc cggcgctgtt ggaaaagctc gagtgctata tcatcaccaa
     4021 tcgcgggctt gccgccccgt tcgatcgtct gccgcccatt cccgatcggt tgctgcggct
     4081 gctggccgag ggtcagccga aagccgcggg ggaacgctgg tggctttttt ccttgcgctc
     4141 catcgagccc aaatgggcgg agcaatggcg cctgcccgcc tggtcgctgg cggggcttgc
     4201 cgcgctcgtc gccgccacgc tgctggcctg gcagctcaac acagcttccc ccctgcgccc
     4261 gcagctcgcc gacgcgggcg cgcaagggct cgcgagctcc gcccaatttc agcgcgcgct
     4321 ggaggcgagc gaaagcaatc tttcgaccca cctcgccagc gtcaaaccca ccggcacgtt
     4381 cttgagcaaa gagcaggcgt ggtgccgcca gtacgaggtg acgcgggcgg atgacacccg
     4441 ccttgcaggt attgcctgcc gcgaccacca cggagcctgg caggtcaagg cgcaggcggt
     4501 cctcgccgcc gccgcgcaga ccagcggcta cgcgccggcg ggcgagggca aggccggcgc
     4561 gcacggtctc gagagcgcgc tcggcaaact catgcaggac gtggtcctct tgcctgccga
     4621 cgaaaagcgc gtgcttgccg gtggatggca accgccgcgt tgatcgctcg ccgatagggg
     4681 cggggccaga cctttgaatt gctgcggggc gcgcgcagac ccccgaattc cccgaggccg
     4741 caatcagggc cggtgcggcg cggagggcgc accgctcaca gggctccccg ttaaccgggc
     4801 gggcccttat ttatcgggct ggaaggagag gtgctggacc acccccggcc agcgcacttc
     4861 agccgatggg gttaacgaca tcaggccccc gctcaccccc gtctccggcg gctgcaccac
     4921 gggctg
//
LOCUS       DASTMT010000490         2478 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_2903059, whole genome shotgun sequence.
ACCESSION   DASTMT010000490 DASTMT010000000
VERSION     DASTMT010000490.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2478)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2478)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2478
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2903059"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            101..982
                     /locus_tag="VFR00_11270"
     CDS             101..982
                     /locus_tag="VFR00_11270"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEU0159890.1"
                     /translation="MSQDFLLSLAQAVSKGLLTGMVYGLMALGLSVIFGVVRVVNFAH
                     GEMMTIAMYIAVALFSAYRLDPLLMLVPIAAVLFALGYVMQAGLINPFINRPEHSQFM
                     LLVAVAMIMVNALLIIFGPDARSVQTAYAYDSFQLGPLIVDATKVYAAVAAAAVAGGL
                     FAFFRYSLTGKAIRACADNYQGALVVGLDVRRLYALTFGLGAACVGAAGAMMVLLLDV
                     TPALGPSYTLLAFVVVITGGLGSMPGALLGGMLIGSTEALAGLLFTPSAKSMFAFGML
                     VLVLLFRPQGILGKSAS"
     gene            979..2001
                     /locus_tag="VFR00_11275"
     CDS             979..2001
                     /locus_tag="VFR00_11275"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEU0159891.1"
                     /translation="MRARWLDGVSSRGPILLVVLLAALILFPVVANDYLITVLILIFY
                     FAYVGQAWNLMMGFAGQLSLGHALYVGLGAYVAAALYVHFGISPWLGLWASVAVAASA
                     GGLIGFLAFRFGVAGVYFAILTIAFAEFARLAFDHWSWVGGSAGFFLPVANYTKNDLI
                     NLRGSPTMFYYVLLAMTAAAFLLCHALLRSRAGYYWLAIRESPEAAQALGINLFRYRM
                     YVVVLSAAMTSLGGIVFAFYYNNLFPEQVFHISRSIELILAPIIGGIGTLFGPIIGAF
                     LLTALAEGLDELMRTVGLDVPGVKQIFYGLCLLVVVMFLPDGVWPPLAKLLRLDGRRA
                     HSRNES"
     gene            2003..>2478
                     /locus_tag="VFR00_11280"
     CDS             2003..>2478
                     /locus_tag="VFR00_11280"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU0159892.1"
                     /translation="MAALLEIKGISKSFSGLKAVQNVSFEVPEGAIVGLIGPNGAGKT
                     TCFNLVAGVYRPNAGEIRLAGTRIDGWRPDQICHAGLGRTFQLVKPFAGLTVMENVVV
                     GALKQCASVRAAKQIAAQVVEQLGLQSKYHAPAGSLTLPDRKRLEVARALATAPRIL"
BASE COUNT          342 a          805 c          777 g          554 t
ORIGIN      
        1 tgcaggtcct caagggcaag gtcaacgtgg tgctgcccaa ggacgccgcc gaggcaacgc
       61 ttaccttccc tgagccgccc tggaacgaca agcgccggac ttgagccagg atttccttct
      121 ctcgcttgcc caggccgtca gcaaggggct gctgaccggc atggtctacg gcctgatggc
      181 gctcggcctg tcggtcattt tcggcgtggt gcgtgtcgta aactttgccc acggcgagat
      241 gatgaccatt gccatgtaca tcgcggttgc gctgttttcg gcctaccggc tcgaccccct
      301 cctcatgctg gtgccgattg cggcggtgct gttcgcgctt ggctacgtga tgcaggccgg
      361 cttgatcaat cccttcatca accggcccga gcatagccag tttatgctgc tggtggccgt
      421 ggcgatgatc atggtcaacg cactgctcat catcttcggg cccgatgcgc gcagcgtgca
      481 gacggcctat gcctatgaca gcttccagct ggggccgctg atcgtcgatg ccaccaaggt
      541 ctatgccgcc gtggcggcgg cggccgtcgc cggcggcctg tttgcctttt tccgctactc
      601 gctgaccggc aaggctattc gcgcctgcgc cgacaattat cagggcgcgc tggtggtagg
      661 gctcgacgtg cgccggctct atgcactgac cttcggtctc ggcgccgcct gcgtgggtgc
      721 cgccggcgcc atgatggtcc tgctgttgga cgtgacaccg gcgctcggtc ccagctacac
      781 cctgctcgcc tttgtggttg tcatcaccgg aggtctcggc tcgatgcccg gcgcgcttct
      841 gggcggcatg ctgatcggca gcacggaagc gcttgccggg ctactgttca cgccttcggc
      901 aaagagcatg ttcgccttcg gcatgctcgt actcgtgctg ctgttccggc cgcagggcat
      961 tctcggcaag agcgcctcat gagggcgcgc tggctcgacg gtgtgtcctc gcgcgggccg
     1021 atcctgctgg tcgtgctgct ggctgcgctc atcctgtttc ccgtcgtcgc caacgactac
     1081 ctgatcaccg ttctgatctt gatcttctat tttgcctacg tcggacaggc ctggaacctg
     1141 atgatgggtt tcgccgggca actctcactt ggtcatgcgc tctacgtcgg cctcggtgcc
     1201 tacgtggccg ccgccctcta cgtgcatttc ggcatcagtc cgtggctcgg tctgtgggcc
     1261 tcggtcgcgg tggcggcaag cgcgggcggg ctcattggtt ttctggcctt tcgcttcggc
     1321 gtggccggcg tctatttcgc catcctcacc attgcctttg ccgagtttgc ccgtctcgcc
     1381 ttcgaccact ggtcgtgggt cggtggctcg gccggctttt ttttgcccgt tgccaactac
     1441 accaagaacg acctcatcaa tctgcgcggc agcccgacga tgttctacta cgtgttgctg
     1501 gcgatgacgg ctgctgcctt cctgctgtgc cacgccctgt tgcgcagtcg ggccggctat
     1561 tactggctgg ccatccgcga gagtcccgag gccgcccagg cgctcggcat caatctattt
     1621 cgctaccgca tgtatgtggt ggtgctgtcg gcggccatga catcgctggg gggcattgtt
     1681 tttgccttct actacaacaa cctgtttccg gagcaggtct tccacatctc gcgctcgatc
     1741 gagctgattt tggcgcccat catcggcggc atcggtacct tgttcggacc catcatcggc
     1801 gccttcctgc tgacggcgct ggccgagggt ctcgacgaac tcatgcggac ggttggcctc
     1861 gacgtgcccg gcgtgaaaca gatcttttat ggcctctgcc tgctcgtcgt cgtgatgttc
     1921 ctgcccgatg gtgtgtggcc gccgctggcg aagctgctgc ggctcgatgg ccggcgcgcc
     1981 cacagccgaa acgagagcta gcatggcggc gttgctggaa atcaagggca tttccaagag
     2041 cttcagcggc ctcaaggccg tgcagaatgt gagcttcgag gtgccggagg gtgcaatcgt
     2101 cggcctgatc ggccccaatg gcgccggcaa gaccacctgc ttcaatttgg ttgcgggcgt
     2161 ctaccgcccg aacgctggcg agatccggct ggccggcacc cgcattgacg gctggcggcc
     2221 cgatcaaatc tgccacgccg gcctcggacg caccttccag cttgtgaagc cgtttgcggg
     2281 tttgacggta atggaaaacg tcgtcgtcgg cgcgctcaag cagtgcgcca gcgtgcgcgc
     2341 cgccaagcag atcgccgccc aggtcgtcga gcagctcggt ttgcagagca aataccatgc
     2401 gccggcggga tcgttgaccc tgccggaccg caaacgcttg gaggtggcgc gcgccctggc
     2461 cacggccccg cgcatcct
//
LOCUS       DASTMT010000491         3138 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_2252214, whole genome shotgun sequence.
ACCESSION   DASTMT010000491 DASTMT010000000
VERSION     DASTMT010000491.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3138
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2252214"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..403)
                     /locus_tag="VFR00_11285"
     CDS             complement(<1..403)
                     /locus_tag="VFR00_11285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0159893.1"
                     /translation="MELSPLRQLRQGMLPKMVSWYDPRLLARVGVRTLVSSVFGQYAD
                     QRLMQAVTDTAEDRELIGRYDYCAVDGASDPQKCLAMDAAGAYWIDYVADVGDGFEPT
                     YALAYLLAQDSLEVRGAGKLRHGEILIMGGDQ"
     gene            complement(588..1388)
                     /locus_tag="VFR00_11290"
     CDS             complement(588..1388)
                     /locus_tag="VFR00_11290"
                     /inference="COORDINATES: protein motif:HMM:NF019896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase family
                     metallohydrolase"
                     /protein_id="HEU0159894.1"
                     /translation="MLTALGSAPTLASGNETWANETIEHMRQHAFDPDAGARPRARGS
                     AVERLDGYMIDEVQIRPRQHARRHDRTEEYHAPRRARVHRESSRKLASLGRSAAPAPA
                     PSNSIAELPKPAEAATAPALPAEQAVASLEPTVLAPIAPAQPKAATAPINWVASPNCL
                     ASPLKSVLAEVAALFGVVRVNSTCRSKTHNARVGGASRSYHLTGNAVDFRIAANTAAV
                     SRFLLGKKSVGGFKHYGFGVFHIDTGPRRTWDGGPRRAWASNRRSRRG"
     gene            complement(2004..2525)
                     /locus_tag="VFR00_11295"
     CDS             complement(2004..2525)
                     /locus_tag="VFR00_11295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019198693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1993 domain-containing protein"
                     /protein_id="HEU0159895.1"
                     /translation="MTLSMYQVAVPPFLQTLGALSAVLAKAEAHAGAKKFDPAVLLAS
                     RLAPDMFPLSRQVQIACDFAKSTVARLAGVAVPSFADEEKSLADLRARIDKTLDYVRS
                     FKPGQIDGSEERVVTITLAGKPVTFKGQPYLVHFVLPNFYFHATVAYAILRHNGVELG
                     KRDFMGSVPGLSV"
     gene            complement(2595..>3138)
                     /locus_tag="VFR00_11300"
     CDS             complement(2595..>3138)
                     /locus_tag="VFR00_11300"
                     /inference="COORDINATES: protein motif:HMM:NF019322.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEU0159896.1"
                     /translation="AERLVMRQQGRGTFVVEHTPAHVLFRFFNMFDAAGELIIPDSRR
                     AGATVAPANDAEQMALQLAPGAEVIRIERVRTRQGSPFICETIRLPQDLFPDLAQLEN
                     LPNTLYDLFQKTYGILLVRTEDRLSATAADADTAGVLNLMPGAPLLRIERTAFGLGDR
                     PIEWRVSLCHLQDAHYLART"
BASE COUNT          516 a         1001 c         1099 g          522 t
ORIGIN      
        1 actggtcgcc gcccatgatc aaaatttcgc cgtggcgcag tttgcccgca cctcgcacct
       61 cgaggctgtc ttgcgccaac aggtaggcca acgcgtaggt gggctcgaaa ccatcgccga
      121 cgtcggcgac gtagtcgatc caataggcgc cggccgcgtc catcgccagg cacttttgcg
      181 gatcgctggc gccgtcgacc gcgcaatagt cgtagcggcc gatcagctcc cggtcttcgg
      241 ccgtatcggt cacggcctgc atcagtcgct ggtcggcgta ttggccgaaa accgaagaga
      301 ccagcgtgcg gacgccgacc cgcgccagca ggcgcgggtc ataccaggag accattttcg
      361 gcagcatgcc ctggcgcagc tggcgtaacg gactgagctc catctcctgc ctcccgcgca
      421 caccctttcg gaccccggcc gtgccggccg ggcctaaggt ggccgtttaa tccggcctaa
      481 ggaagaaggt gcaaattgcc cggcacgcag gcgtggccga aatatcccac cacgcctgcc
      541 gtaccctgca gcgtcttcag cccgtcggcg gctgccgcag cgccgcgcta gccgcggcgg
      601 ctccgccggt tagaggccca ggcccggcgc gggccgccgt cccacgtccg gcgtgggccg
      661 gtgtcgatgt ggaacacgcc gaagccgtag tgcttgaagc cgccgacgct ttttttgccc
      721 agcagaaacc ggctgaccgc ggccgtgttg gcagcgatac ggaaatcgac ggcattgccg
      781 gtcagatggt aggagcgcga cgccccgccg acccgcgcgt tgtgcgtctt gctgcggcag
      841 gtcgaattga cccgcacgac gccgaacagc gccgccacct cggcgagtac gctcttgagc
      901 gggctggcca ggcagttggg tgaggcgacc caattgattg gcgcagtcgc cgctttgggt
      961 tgcgctggag cgatgggcgc cagcaccgtc ggttcgaggc tcgcgacggc ctgctcggcg
     1021 ggcaacgccg gcgcggttgc tgcctccgcg ggtttgggca gctcggcaat cgagttggac
     1081 ggtgcgggcg ccggcgccgc gctgcgaccg aggcttgcga gcttgcggct gctttcccgg
     1141 tggacgcgcg cccggcgcgg cgcatgatac tcctccgtgc ggtcgtgtcg gcgcgcatgc
     1201 tggcggggcc ggatctgcac ctcgtcgatc atatagccgt cgaggcgttc caccgccgag
     1261 ccacgtgcgc gcgggcgcgc gcccgcgtcc gggtcgaacg cgtgctgacg catgtgctcg
     1321 atggtctcgt tggcccaggt ctcgttgccg gaggccaacg tcggcgcgct gccgagggcg
     1381 gttaacacga ataaaacgcc cggcagcaaa atcgtctgcg gcctccaccg gcagggcgcc
     1441 gaaccctccc aaagcggccg tgatgaattc gctgcgagcg cgtgaacggg tacagtcatc
     1501 gcctgaaact ccatgcgtga ccgaggcacg cgtgctgcga cctcggttcg gttttgccga
     1561 ccccgctgac agtcctgctg tgtgtggaat ccccccgtca cgagagaccc aacggcccct
     1621 cgttagcgtg caaagggggg cttatgtggc caaggaagtg cggcgaatgc gcgcaaaaag
     1681 ctgaggcgtg acaaggcgat gggagatttt cttcaacttt cagttgcacc gcggcgcccc
     1741 gcggctgacc caaaacaggc ccggtggccc gggaaaatgg cgcccctgcg gcgtttgccg
     1801 ggcgtcgcgc ggtgcgctgc gccgccaggg gaggggaggc agctgggggc ccgacaaatg
     1861 gcgcaggcgc gcgcgggctc acacgggacg gccggcgccg tcgcggcttt gccgacggcc
     1921 gggtgtggtg gggcacccgc gcgtcgtccg gccgggtcgt ttaacggtgg cgcggaggcc
     1981 ctacggcgct ggatgggcgc cggctagacg ctcaagcccg gcacgctgcc catgaaatcg
     2041 cgcttgccaa gttccacgcc gttgtgacgc aggatggcgt aggcgacggt ggcgtgaaag
     2101 tagaagttgg gcagcacgaa gtggaccagg tagggctggc ctttgaaggt gacgggcttg
     2161 ccggccaacg tgatagtgac aacgcgctcc tccgagccgt cgatctggcc gggcttgaag
     2221 gagcgcacgt agtcgagcgt cttgtcgatg cgggcgcgca aatcggccag gcttttctcc
     2281 tcgtcagcga agcttgggac ggcgacgccc gccaggcgtg ccacagtgct cttggcaaaa
     2341 tcacaggcga tctgcacctg ccggctcagc ggaaacatat cgggcgccag gcgtgaagcc
     2401 agcaacacgg cgggatcgaa cttcttggcg cccgcatggg cctctgcctt ggcgagtacg
     2461 gcggagaggg caccgagcgt ttgcaggaac ggcggcacgg cgacttggta catggacagg
     2521 gtcatcgctt cgtctctttc acaagatgtc gtggccgcgc gtcttgcggg cgtgttgccg
     2581 aaggacgcgc ggtgttacgt acgtgccaga taatgcgcgt cctgcaaatg gcacaggctc
     2641 acgcgccatt cgatcggccg gtcgccgagg ccgaatgcag tgcgctcgat tcgcagcaaa
     2701 ggcgccccgg gcataaggtt caagacgccg gccgtgtcgg catctgcggc cgttgccgac
     2761 aggcgatcct cggtgcgcac cagcagaatg ccgtaggtct tttgaaagag atcataaagc
     2821 gtgttgggaa ggttttcgag ctgggcgaga tcgggaaaca agtcctgggg cagcctgatg
     2881 gtttcgcaaa taaaggggct gccctggcgg gtgcgcacgc gctcaatgcg aatgacctcg
     2941 gcgcctggtg ccagctgcag tgccatctgc tcggcgtcgt tggcgggcgc aaccgtcgcc
     3001 cccgcgcgcc gactgtcggg aatgatcagt tcgccagcgg catcgaacat gttgaagaag
     3061 cggaagagca cgtgtgccgg ggtgtgctcg acgacgaagg tgccgcgacc ttgctgacgc
     3121 atcaccaacc gctccgcg
//
LOCUS       DASTMT010000492         2827 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_4737078, whole genome shotgun sequence.
ACCESSION   DASTMT010000492 DASTMT010000000
VERSION     DASTMT010000492.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2827)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2827)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2827
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4737078"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1033
                     /locus_tag="VFR00_11305"
     CDS             <1..1033
                     /locus_tag="VFR00_11305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007598984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEU0159897.1"
                     /translation="IFRTKGRTYIYAANGHGGWEAALTNVLSRGDLVLALESGRFATG
                     WGEMASMLGVEVEVLKGDWRRAVDPAVVEERLRRDKAHRIKAILVVQIDTASGVVNDV
                     AAIRKALDAAQHPALLMVDAVASLGCMPFDMDAWGVDVTMAGSQKGLMTPPGLAFVAA
                     NDRARAAHKTANLRTLYWDWTFREGEIHYQKYCGTPPEHLLFALRKALDLLLAEGLEG
                     AFRRHALLAQATRVAVERWAEGQVLAFNIANPAERSNSITNVLMQGRDPRPLLAYCRD
                     KCGVILGVGLGQLEGKAFRIAHMGHCNAPMLLGTLGAVEMGLKALRIPHGRGGVEGAI
                     QYLGETVPA"
     gene            complement(1099..2529)
                     /locus_tag="VFR00_11310"
     CDS             complement(1099..2529)
                     /locus_tag="VFR00_11310"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018425587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEU0159898.1"
                     /translation="MYTSLEHYIDGNWVKASGAKSQEVINPATSKAIGELSHASRGDL
                     DKALAAADKGFKTWRKVSSYERGKILRKAADLVRARADEIAKVLTLEQGKVFMEAKLE
                     VQSAGDILDWYAAEGQRAYGRIIPARADGVRNMVIVEPIGPVAAFTPWNFPVTQAVRK
                     IAAALAAGCSIIIKCPEETPGSPIGLVKALHDAGVPPGVLNLVYGVPAEISEYLIPHP
                     SIRKVSFTGSVPVGKHLNALAASHMKRATMELGGHAPVLVFDDADVEGAAKLMGAFKY
                     RNAGQVCVSPTRFFVHDKVYDNFVGKFIDIAKKTKVGDGMAADTRMGPLANPRRINAI
                     EAFVADAQDKGAKVEAGGKRIGNQGNFFEPTVLTDVPESARIMSEEPFGPVAVMLRFK
                     ETDDVLDRANKLPFGLASYAFTKDAKVATKVADALESGMVSINHFGIALPETPFGGVK
                     DSGFGHEGGTEGLQVYLQSKFVSHLG"
BASE COUNT          478 a          915 c          932 g          502 t
ORIGIN      
        1 cattttccgc accaagggtc gcacctacat ctacgccgcc aatggtcacg gcggctggga
       61 agcggcgctc acgaacgtgc tgagccgcgg cgatctcgtg ttggcgctcg agagcggtcg
      121 cttcgccacc ggttggggtg agatggcctc catgctcggg gtggaggtag aagtcctcaa
      181 gggtgactgg cgccgcgccg tcgatccagc cgtcgtcgag gagcggctgc gccgggacaa
      241 ggcgcatcga atcaaggcga tcctcgtcgt ccagatcgac acggcctcgg gcgtggtcaa
      301 cgatgtagcg gccattcgca aggcgctcga cgcggcccaa catcccgccc tgctcatggt
      361 cgatgcggtc gcctcgcttg gctgtatgcc ctttgacatg gacgcgtggg gggtggacgt
      421 gaccatggcg ggctctcaga agggcctcat gacgccgcca ggccttgcct tcgtcgccgc
      481 caatgacaga gcccgcgccg cacacaaaac ggcgaacctg cgtaccctct attgggactg
      541 gacgtttcgc gaaggggaga tccactacca aaagtattgt gggacgccgc cggaacattt
      601 gctgttcgcg ttgcgcaagg ccctcgacct gctgctggcc gaagggctcg aaggagcctt
      661 tcgccggcac gcgctgctgg cccaggcaac gcgcgttgcc gtcgagcgct gggccgaggg
      721 ccaggttttg gctttcaaca ttgccaatcc ggccgaacgc tcgaactcga tcaccaacgt
      781 gttgatgcag ggccgcgacc cacggccgct gctcgcctat tgccgcgaca aatgcggcgt
      841 catccttggg gttgggctcg gccagctgga aggcaaggcc tttcgcatcg cccatatggg
      901 gcactgcaac gcgccgatgc tgctcggcac cttgggggcc gtcgaaatgg gtctcaaggc
      961 gctgcgaatt ccgcacggcc gcggcggcgt ggagggcgcc atccaatatc tgggcgagac
     1021 cgtgcccgcc tgaggccggc cgccgcgccg gggcggcggc cgcaaacggt ggttgggctc
     1081 ggcgcgcgag agcccgcgct atccgaggtg gcttacaaac ttggactgca ggtaaacctg
     1141 cagaccctcg gtgccaccct cgtgaccgaa accggaatcc ttgaccccgc cgaacggtgt
     1201 ctcgggaagc gcaatgccga aatggttgat cgacaccatc ccgctctcga gcgcgtcggc
     1261 gaccttggtt gccacctttg cgtccttggt gaaggcgtag ctggcgagcc cgaagggcag
     1321 cttgttggcc cggtccagga catcgtccgt ctccttaaag cgcagcatca cggcaacggg
     1381 gccgaacggt tcctcgctca tgatgcgggc gctctcgggc acgtcggtta ggacggtcgg
     1441 ctcgaagaag ttgccctgat tgccaatgcg cttgccgccg gcctcgacct tggcgccctt
     1501 gtcctgcgcg tcggctacga acgcctcgat ggcattgata cggcgcggat tggcaagcgg
     1561 acccatgcgg gtatcggcag ccatgccgtc gccgaccttc gtctttttgg cgatgtcgat
     1621 gaacttgccg acaaagttgt catagacctt gtcgtgcacg aaaaagcgcg tgggcgaaac
     1681 gcagacctgg ccggcattgc ggtatttaaa agcgcccatc agcttggccg cgccctccac
     1741 atcggcgtca tcgaaaacca ggaccggcgc atgcccgccc agctccatcg tggcgcgctt
     1801 catgtgggag gctgccaagg cgttgagatg tttgccgacc ggcaccgagc ccgtaaagga
     1861 aaccttgcgg atgctgggat gcgggatcag gtattcggaa atttccgcgg gcaccccata
     1921 gacgaggttc agcacgcccg ggggcacgcc tgcgtcgtgc agggccttta cgaggccgat
     1981 cggcgacccg ggggtctcct cgggacactt gatgataatc gagcacccgg cggccagcgc
     2041 agccgcgatc ttgcgcaccg cttgcgtgac gggaaaattc cagggcgtaa atgcggccac
     2101 cgggccgatc ggctccacga tcaccatatt gcgcacgccg tcggcacgcg cgggaatgat
     2161 acggccatag gcgcgctgcc cctcggcggc ataccagtcg aggatgtcgc cggcgctctg
     2221 cacctcgagc ttggcttcca tgaagacctt gccctgctcc agggtcaaaa ccttagcgat
     2281 ctcatcggcc cgagcccgca ccagatcggc ggccttgcgc aatatcttgc cccgctcgta
     2341 ggaggacacc ttgcgccagg tcttgaagcc cttgtcggcg gcagccaggg ccttatcgag
     2401 gtcaccccgc gaggcatggc tgagctcgcc gatggctttg ctggtcgcgg gattgatcac
     2461 ctcctggctt ttggcgccgg aggccttgac ccagttgccg tcaatatagt gctcaaggga
     2521 cgtgtacatg tgcgctacct ctaggcttgg ggactgcaga cgttggggcg aataacgaca
     2581 gatagggcgg cttgcgcaag cggattcgcc gccctcccgg tcaaacgagc gccttgccgg
     2641 cccggtcgcg gcgagcacgg cggcggcgac cgccgctggg gcctgcggaa tatcacgctg
     2701 gatatagcga ggcgaccgcc tcttgccaat cgtgaccggg tttcgcaggc cgctccaccg
     2761 ggcccaatgg gccgccgggc cgcgggcggg tccgcggccc gccagccttt tcgggacgtc
     2821 agcgcca
//
LOCUS       DASTMT010000493         3176 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_1923044, whole genome shotgun sequence.
ACCESSION   DASTMT010000493 DASTMT010000000
VERSION     DASTMT010000493.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3176)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3176
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1923044"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..916
                     /locus_tag="VFR00_11315"
     CDS             <1..916
                     /locus_tag="VFR00_11315"
                     /inference="COORDINATES: protein motif:HMM:NF018534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding protein 1C"
                     /protein_id="HEU0159899.1"
                     /translation="TITVRTALAQSLNIPAVKVLDALGPGRLYGRLQQLGASPVLPKG
                     AEPTLAMALGGLGLRLSDLAMLYTALARGGDAVALRWRRQPAEPRGELPPQRLLTPVA
                     AWYVTDILRHAPAPANARPGQIAYKTGTSYGFRDAWAVGYDGRHTVAVWVGRPDGTAT
                     PGLAGRAAAAPLLFDAFARLSPQRTPLPPAPPGALRLATAQLPPPLQRFGSAEEQDAG
                     AYLDPPVQIAFPPDRAELAFEDIDGGGFAVKAQGGALPLVWLRDGVPLTSDAAKRETE
                     VSVSGRGFFTLTVIDAKGRDDRVTVRIK"
     gene            1058..2050
                     /locus_tag="VFR00_11320"
     CDS             1058..2050
                     /locus_tag="VFR00_11320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015596272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly(3-hydroxybutyrate) depolymerase"
                     /protein_id="HEU0159900.1"
                     /translation="MPNLGVDINATSVSGLSAGAYMAGQFQVAHSHMVVGAGIVAGGP
                     YGCAESAYADAMPGPGAAFINLSKAINGCMLDAMQAWGVPDPPRLAERARSLALKDRI
                     DPIEGLAQDRVYLFSGSNDRTVLPSIVRAAAQLYEQLGIPPANIKFVSDLPAGHAFLT
                     KGSGIACGRTGEPYLSNCNYDQAGEILQQIYGPLRPRVASPTGDFIVFDQREFTRDQP
                     SHGLGTSGVAYVPVDCRTSQGCRVHVVFHGCNQHRDKVGDAFVRDAGFPAWADSNRLV
                     LLFPQVTTTTVNPQACWDWWGYTGRDYLTRNGPQIEAVRRMLDRLSGHSSVSRS"
     gene            2206..3174
                     /locus_tag="VFR00_11325"
     CDS             2206..3174
                     /locus_tag="VFR00_11325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012333244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HEU0159901.1"
                     /translation="MLAPRETLKICFAHAAYRMAERFAQRGTGIAHFQVHSLDELKAR
                     VPEAHVVVVSMMWRNELIAIARNLQFIQSISAGLDQYDRDRIRQGGIRLASAAGVNAE
                     AVAQHAMALILALKRQLHTGRDNQAAKVWRGMISEIAAREDQLNGKTLLIVGIGRIGA
                     GLARLARAFDMHVIATRRNPDAGAQGAHAVYAHDRLPELLPQADVVALTCPLTPQTEN
                     LIDAKAIGLLRPSAVLINVARGRVVDEAALIEALQAKRLAAAGLDVTREEPLPPASPL
                     WTMPNVLLTPHTAGETQSYEDSVIDILLENLERMWRGETSLRNEVI"
BASE COUNT          490 a         1130 c         1023 g          533 t
ORIGIN      
        1 caccatcacc gtccgcacgg ccttggccca gtcgctgaac attcccgccg tcaaggtcct
       61 cgatgccttg ggacccggca ggctctacgg ccggctccag cagctggggg cgagccccgt
      121 gctgcccaaa ggggccgagc cgacgctcgc catggcgctc ggcgggctcg gcctgcggct
      181 atcggatttg gccatgctct acaccgcgct cgcccgcggt ggcgacgcgg tggcgttgcg
      241 ttggcggcgc cagcccgccg agcctcgggg cgagctgccg ccccagcgcc tcttgacgcc
      301 ggtcgccgcc tggtacgtga ccgacatcct gcgccatgcg ccggcgcccg ccaacgccag
      361 gcccggccag atcgcctata agacagggac ctcctacggt tttcgcgatg cctgggccgt
      421 cggctacgac ggtcgccata ccgttgccgt ttgggtcggc cggccggacg gcacggccac
      481 gcccggcctt gccggacggg cggcggccgc gcccctcctg tttgatgcct tcgcgcgcct
      541 ctccccacag cgcacgccct tgccgccggc gccgcccggc gccttgcgcc tggccacggc
      601 gcagctgcca ccccccttgc agcgcttcgg cagcgccgag gagcaggatg cgggagccta
      661 cctcgacccg cccgtgcaaa tcgcctttcc tccggatcgc gccgagctcg ctttcgagga
      721 catcgacggg ggcggattcg ccgtcaaagc ccaggggggt gccttgccgc tggtgtggct
      781 gcgggacggc gtgccgctga cgtcggacgc ggccaaacgc gagaccgaag tgtcggtcag
      841 cggccggggg ttcttcaccc tcacggtgat cgacgccaag ggacgtgacg atcgggtgac
      901 cgtgcgcata aagtgagcct gccggcgacg accgccaaac atcggccgta ccggtgaggg
      961 ttgatgcggc atggcactcg ccggcgcacc ttggctctgc gcagtcgccc tagcggcgat
     1021 ggcctccttg cttgcctgca attcccaaga cgcggccttg ccgaacctcg gggtcgacat
     1081 caacgccacc tccgtttccg gcctctcggc gggggcttat atggcgggcc agttccaggt
     1141 cgcccattcg cacatggtgg tcggcgccgg catcgtcgcc ggtggaccct atggctgcgc
     1201 ggagagcgcc tacgcggacg ccatgccggg cccgggcgcg gctttcatca acctgtccaa
     1261 ggccataaac gggtgcatgc tcgatgccat gcaagcctgg ggcgtacccg atccgccgcg
     1321 gctggccgag cgtgcgcgca gcctggcgct gaaggaccgc atcgatccga tcgaggggct
     1381 ggcgcaggac cgcgtttatc ttttcagcgg cagcaacgac cgaaccgttc tgccctcgat
     1441 cgtgcgggcg gcggcccagc tttacgagca gcttggcatc ccgcccgcca atatcaaatt
     1501 cgtcagcgac cttcccgccg gccacgcctt cttgaccaag ggcagcggca ttgcctgcgg
     1561 tagaacgggc gagccctacc tcagcaactg caactacgat caggcgggcg aaattctgca
     1621 gcaaatctac gggcccctgc gtcccagggt tgcctccccc actggggatt tcatcgtctt
     1681 cgaccagcgc gagtttaccc gtgaccagcc cagccacgga cttgggacca gcggcgtcgc
     1741 ctatgtcccg gtcgattgtc gcaccagcca gggctgccgc gtgcatgtcg tcttccatgg
     1801 ctgcaaccag catcgcgaca aggtgggaga tgccttcgtt cgggacgccg gctttccggc
     1861 ctgggccgac agcaaccgct tggttttgct ctttccccag gttaccacca ccaccgtcaa
     1921 tccgcaggct tgctgggatt ggtggggtta caccgggcgc gactacctga cgcgcaacgg
     1981 cccgcaaatc gaggcggtgc gacgcatgct ggaccggctc tccggacatt ccagcgtcag
     2041 ccgtagttag cggcgcggcg gaccgaccgt cgttgtcccc tcgccccctc cggctttcgg
     2101 ccaaccgccg cgcgcggcgc caggcgggga acggtggcct tcgcccgcct tctgttgcat
     2161 gctccgcccg cacatcggcc atcgcaacga tcgggatatc cgcgcatgct cgcccctcga
     2221 gagacactga aaatctgttt cgcccacgcg gcctaccgca tggccgagcg gttcgcccag
     2281 cgcggtacgg gcatcgccca tttccaggtg cactcgctcg acgaactcaa ggcacgcgtt
     2341 cccgaggccc atgtcgtcgt cgtctcgatg atgtggcgca acgagctcat cgcaatcgcg
     2401 cgtaatctgc agttcatcca gtcgatcagc gccggtctcg atcaatacga ccgcgatcgc
     2461 atccgccaag gcggcatacg cttggcgagc gccgctggag tgaatgccga ggctgtcgcc
     2521 cagcatgcca tggcgctgat cctggcgctg aagcggcagc tgcataccgg tcgcgacaac
     2581 caggccgcta aagtgtggcg tggcatgatc tccgaaatcg ccgcacgcga agaccagctc
     2641 aacggtaaaa ccctgttgat tgtggggatc ggccgcatcg gcgcgggctt ggcgcggctc
     2701 gctcgggctt tcgacatgca cgtgatcgca acccggcgca atccggacgc gggcgcccag
     2761 ggagcgcacg ccgtctacgc acatgaccgg ctgcccgagc ttctgccgca ggccgacgtc
     2821 gtcgcgctca cctgtccgct gacgccgcaa actgagaatt tgatcgacgc caaggccatt
     2881 ggcctgctgc ggccctccgc cgtcctgatc aatgtcgctc ggggccgggt ggtcgacgag
     2941 gcagctttga tcgaagccct gcaggccaag cggcttgcgg ccgccggcct cgacgtgacc
     3001 cgcgaagagc cgctgccgcc ggcctcgccg ctctggacca tgcccaacgt cctcctcacg
     3061 ccgcatacgg cgggcgagac gcagagctat gaggatagcg tgatcgatat cctgttggag
     3121 aacctggagc gcatgtggcg cggggagacg agcttgcgca acgaggtgat ttaaag
//
LOCUS       DASTMT010000494         3619 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_4216485, whole genome shotgun sequence.
ACCESSION   DASTMT010000494 DASTMT010000000
VERSION     DASTMT010000494.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3619
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4216485"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..551)
                     /locus_tag="VFR00_11330"
     CDS             complement(<1..551)
                     /locus_tag="VFR00_11330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015668142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1445 domain-containing protein"
                     /protein_id="HEU0159902.1"
                     /translation="MMRVSDPGEARKIIRSGGYAGHTAGVAAKYVQGNVCILPKELAG
                     EFAAFCQRNPKPCPVIGMAAPGDPTLPDLGDIDIRTDVPRYRVFKDGKLIDEPTDIRQ
                     YWRADLVTFVLGCSFSFELPILEAGIRLSHIEDNTTVPMYRTNIDCVPAGPFRGKMVV
                     SMRAFSPADAIRVIQITSRFPAV"
     gene            complement(729..2522)
                     /locus_tag="VFR00_11335"
     CDS             complement(729..2522)
                     /locus_tag="VFR00_11335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002711298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HEU0159903.1"
                     /translation="MRSSSSTQSAPLGHVVAIGGSSASIRLTPAAAASPEPARITIGN
                     FLGIETDASRVVAVITHVDGSDKVAGEPATGGLLARADLLGEIRTHADGTFVFQRGIT
                     RYPVIGEGAQLLSGDSLRMVFDTCGAAVIKIGKLQQDASIEATVKVDDMLGKHFALLG
                     STGVGKSSGVAVLLRAILEVRPNLRIFLIDPHNEYGHSFRERAQILTPKNLKLPFWLF
                     NFEETVDVFFRGRPGLEDEVEILAQLIPQAKNQFAAARGAESQTLRKVDSSQTGYTVD
                     TPVPYRLSDLVNLIDERMGKLENRPSTSKFSRLVARIETVRQDPRYAFMFDNANVGGD
                     TMAEVVGQLFRLPANGKPMTVMQLAGFPSEVVDSVVSVLGRMAFDFGLWSDGAVPLLF
                     VCEEAHRYAPGDKSIGFGPTRKAISRIAKEGRKYGVFLGMVTQRPAELDATIISQCNT
                     LFVMRMANERDQNIIRSAVSDAAASLLAFLPSLGTREVFAFGEGVALPTRLRFSELPA
                     PMIPRSESELSIRVDSNRGVDTAFIHSVVERWRGATMGNKRKLGEPTPEQAKAPGEWG
                     AFVTEAEQPLLAAATRPVHAATSLRASLLRK"
     gene            2748..3314
                     /locus_tag="VFR00_11340"
     CDS             2748..3314
                     /locus_tag="VFR00_11340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018317908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEU0159904.1"
                     /translation="MSASLRDAMRAMAYNNAWANHRLHAACALLSPAEFEAPRTGFFP
                     SLKATLNHILIIDWFYVDALEGGSLGPAAWANQVPCATIAELASEQARVDRRLLAFCE
                     ALTAEALSGWSRVRRQDRVQVERTDRLLLHLFQHQVHHRGQAHAMLSATSVKPPQLDE
                     FFSADEAPLRAAEFAELGWTEVRVWSGP"
     gene            3436..>3619
                     /locus_tag="VFR00_11345"
     CDS             3436..>3619
                     /locus_tag="VFR00_11345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014491612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEU0159905.1"
                     /translation="MQNSEKIWQLVDARKDAYEALSDRVWEMPEICYTEFRSVAEHRA
                     MLELEGFKITENLAGIP"
BASE COUNT          603 a         1146 c         1236 g          634 t
ORIGIN      
        1 tgcaccgccg gaaagcgcga ggtgatctgg atgacccgaa tggcatccgc cgggctgaac
       61 gcgcgcatcg agaccaccat cttgccccgg aaaggtccgg cgggcacgca gtcgatgttc
      121 gtgcggtaca tgggtacggt ggtgttgtcc tcgatatggc tgaggcggat gcccgcctcc
      181 aggatcggca gctcgaagga gaacgagcag ccgagcacga acgtgacgag gtcggcgcgc
      241 caatattggc gaatatcggt gggttcatcg atcaacttgc cgtccttgaa gacgcgatag
      301 cgcggcacgt cggtgcggat gtcgatgtcg cccaggtccg gcagggtggg atcgccgggc
      361 gcagccatgc cgatcaccgg acacggcttg gggttgcgct gacagaacgc ggcaaattcg
      421 ccggccagct ccttgggcaa gatgcacacg ttgccctgca cgtatttcgc cgccacgccg
      481 gcggtgtggc cggcatagcc gccagagcgg atgatcttgc gcgcctcgcc ggggtcgcta
      541 acccgcatca tggcgctgcc ggccgcgccg ggtacctctt gttgcatctc gggcctcccc
      601 tgctggatgc gcgccttctc gccagacggc aacgcgccgc gacggctttt atagcacgcc
      661 gggccgcatt ggggtcaggg cggattggcg gccgcccgag gggagggggt tgcgacggcg
      721 cggctgcgtt atttgcgcag aaggctggcg cgtaagcttg tggccgcatg caccgggcgc
      781 gtggcggcgg ccagcaacgg ctgctcggcc tcggtgacaa aggcgcccca ttcgcccggc
      841 gccttggctt gctcgggcgt cggctcgccg agcttgcgct tgttgcccat ggttgccccg
      901 cgccagcgtt ccacgacgct gtgaatgaaa gccgtatcga cgccgcggtt ggaatcgacg
      961 cggatgctca gttccgattc actgcgcggg atcatgggtg cgggcagctc gctaaagcgc
     1021 aatcgggtcg gcagtgccac gccctcgccg aacgcgaaca cttcccgggt acccagcgag
     1081 ggcaagaacg ctagcaggct tgcggccgcg tcggagaccg ccgaacggat gatgttctgg
     1141 tcccgctcgt tcgccatgcg catgacgaag agggtgttgc attgcgagat gatggttgca
     1201 tcgagttcgg cgggccgttg tgtgaccatg ccgaggaata ccccgtactt gcggccctct
     1261 ttggcgatgc gcgagatcgc cttgcgcgtg gggccgaaac cgatgctctt gtcccccggc
     1321 gcgtagcggt gcgcctcctc acagacgaac aggagcggca ccgcgccgtc gctccacagc
     1381 ccgaagtcga aggccatgcg cccgagcacg gaaacgacgg agtccaccac ctcggagggg
     1441 aagccggcca gctgcatcac ggtcatcggc ttgccgttgg cgggcaagcg gaacagctgg
     1501 ccaacgacct cggccatcgt gtcgccgccc acgttggcat tgtcgaacat gaaggcgtag
     1561 cgcgggtcct ggcgcaccgt ttcgatgcgg gctaccagcc ggctgaattt cgaggtcgag
     1621 gggcggttct cgagcttgcc catgcgctca tcgatcaggt tgacgaggtc ggacaggcgg
     1681 tagggcacag gggtgtcgac ggtgtagccg gtctggctcg aatcgacctt gcgcagtgtt
     1741 tggctctcgg cgccgcgcgc cgctgcaaat tggtttttgg cctgcgggat cagctgggcg
     1801 aggatttcca cctcgtcctc gagccccggg cgtccgcgga agaacacgtc gaccgtctct
     1861 tcgaagttga acagccaaaa cggcagcttc aggttcttcg gggtgaggat ttgcgcgcgc
     1921 tcgcggaagg agtggccgta ttcgttgtgc ggatcgatca gaaagatgcg caggttcggg
     1981 cggacctcga ggatggcgcg cagcagcacc gccacgccgc tcgacttgcc gacgcccgtg
     2041 ctgcctaaca gggcgaagtg cttgccgagc atgtcatcga ccttgacggt ggcctcgatc
     2101 gaggcatcct gctgcagttt gccgatcttg atcaccgctg cgccgcaggt gtcgaacacc
     2161 atgcgcagac tatcgccgct cagcagctgg gcaccctcgc cgatcacggg gtagcgcgtg
     2221 atgccgcgct ggaagacgaa cgtgccatcg gcgtgcgtgc ggatctcgcc cagcagatcg
     2281 gcccttgcca aaagtccccc cgtcgccggc tcgccggcga ctttgtcgct gccgtcgacg
     2341 tgggtgatca ccgccaccac gcgggaggcg tccgtctcga tgccgaggaa gttgccgatc
     2401 gtgatccgcg ccggctcggg gcttgcggcg gcagcgggcg tcaggcggat gctggccgag
     2461 gagccgccga tcgcgaccac gtggccgagc ggggcggact gagtgctgga tgaggaacgc
     2521 ataggcaacc gagaccggga tgacgagatt gcccactcaa tacgcaaacc attaagaaag
     2581 cgcgtaaggg gcgcgtcgcg ggtccttgcg cgccttcctc gtgagtgctt gcgcgccttc
     2641 tcattagtgt atgccatggg gcattgccac ggtgcccggc ggcgcgtcac gttgccgccg
     2701 cgttcgcggc gcggcaattg cggaaaagca caggaggtcc aatcccaatg tcagccagtt
     2761 tgcgtgacgc catgcgcgcc atggcttaca acaacgcctg ggccaaccac cgcctgcatg
     2821 cggcctgcgc cctgctctcg ccggccgaat tcgaggcgcc gcgcaccggc ttctttccct
     2881 ccctgaaagc aacgcttaac cacatactga tcatcgactg gttctacgtc gatgcgctgg
     2941 agggcggcag ccttggcccg gccgcctggg ccaaccaggt gccctgcgcc accattgccg
     3001 aattggcaag cgagcaggcg cgcgtcgatc gccgactgct cgccttttgc gaggccttga
     3061 ccgcggaggc tctcagcggt tggagccggg tccgccgcca ggaccgggtg caagtcgagc
     3121 gcaccgaccg cctgctgctg catctgttcc agcaccaagt gcatcatcgt ggacaagccc
     3181 acgccatgtt gagcgcaacc tcggtgaagc cgccgcagct cgatgagttt ttctccgccg
     3241 acgaggcgcc gctgcgcgcc gccgagtttg ccgaactggg ctggaccgag gtgcgggtct
     3301 ggtcgggccc atgatcgccg cgcggccgcg gggcacgccg cggcaagctg tctctggggg
     3361 cgggaaaaaa tatcggctcg ggtgcttttg tggcgcgccc agccagttta ggttcgcggc
     3421 aacaggagag gcccgatgca gaacagcgaa aagatttggc agctcgtcga cgcgcgcaag
     3481 gatgcctatg aggccctgtc cgatcgtgtc tgggaaatgc cggagatttg ctacaccgaa
     3541 ttccgttccg ttgccgagca tcgcgcgatg ctggaactgg aaggcttcaa aatcacggag
     3601 aacctggccg gcatcccga
//
LOCUS       DASTMT010000495         1728 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_5720142, whole genome shotgun sequence.
ACCESSION   DASTMT010000495 DASTMT010000000
VERSION     DASTMT010000495.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 1728)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1728)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1728
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5720142"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..627
                     /locus_tag="VFR00_11350"
     CDS             <1..627
                     /locus_tag="VFR00_11350"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEU0159906.1"
                     /translation="AWLTTALAFIPMRLLPGVAAQLPGGEAATVTSFVTHLFVHGDTT
                     HLIINMAWLLAFGAPLARRTDPVRFLVFFALCGIAGSLFYVLVNGRVLTLVIGASGAI
                     SGLMGGAMRFFFRALAPGRPLTAPLHAAPLLSLAETLRDKRILSAMLGWTVLNIVLAW
                     GAAGLTDAAGIAWEAHVGGFYAGLLSYGLFDRRAPDQEVEPSNAAGAE"
     gene            790..1221
                     /locus_tag="VFR00_11355"
     CDS             790..1221
                     /locus_tag="VFR00_11355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018391296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEU0159907.1"
                     /translation="MNVAAILRQKGRAVTTAAPGATLLEIAAKLAAKRIGAIVVVGPQ
                     GEVAGIISERDIIRALAAHGPECLTSSVAESMTKDVVTCQETDTLDELMAMMTARRFR
                     HLPVQTDGALVGIISIGDVVKHHVAEVEMEATAMRDYIAHS"
     gene            complement(1267..>1728)
                     /locus_tag="VFR00_11360"
     CDS             complement(1267..>1728)
                     /locus_tag="VFR00_11360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTE family protein rssA"
                     /protein_id="HEU0159908.1"
                     /translation="VSVCRALGARYVIAVNVNSDSCGHGTVVPQMEMLTAAEDAPVSA
                     VEPAATYGVGGMRKLLKRQFFGRGDGGPGISRVMMEAFNIVQDRIARSRLAGDPPDIV
                     ISPKLPQFGLFDFHQADALIRGGKLAAQRELEDIERELALRRPPRAMARSA"
BASE COUNT          279 a          568 c          562 g          319 t
ORIGIN      
        1 gcctggctga cgacggcgct ggccttcatt cccatgcggc tgctgcccgg ggtggccgcg
       61 caattgccag gcggcgaggc cgccaccgtc acctccttcg tcacgcacct gttcgtgcat
      121 ggtgacacca cgcacctcat catcaacatg gcatggttgc tggcctttgg cgccccactg
      181 gcacggcgca cggaccccgt gcgctttctc gtcttcttcg cgctgtgcgg catcgccggc
      241 tcactgtttt acgtcttggt taacggccgc gttctgaccc tggtgatcgg cgcctccggc
      301 gccatttcgg ggctgatggg cggggcgatg cgcttctttt tccgcgccct ggcccccggc
      361 cgaccgctca cggcgccgct gcatgcggcg ccgctcttga gccttgccga gacccttcgc
      421 gacaagcgca tcctgtcggc catgctcggc tggaccgtgc tcaatatcgt gctggcttgg
      481 ggtgcagccg gcctcacgga tgcggccggc atcgcctggg aagcccatgt tggcggcttt
      541 tatgcgggtt tgctcagcta tggtctgttc gaccggcgcg ccccggacca agaggtcgag
      601 ccaagcaatg ctgcgggcgc ggaataggtc tcttgcggcc cttcatgaag ttgcccacaa
      661 tcccctccag gctcgcgcaa tcgcacagca ggccgacaga ttgcggcgca ggaggacggc
      721 aagctagttc gggggacctc ttcgtggcac ctgtctaatc gctgtggcag cgaatgggag
      781 gacattgcca tgaacgttgc cgccatcctt cggcaaaagg gtcgcgccgt caccaccgca
      841 gcacccggcg cgacgttgct tgagattgcc gccaagctcg cggcgaaacg cattggcgcc
      901 atcgtcgtcg tcggtcccca aggcgaggtg gccggcatca tttccgaacg cgacatcatc
      961 cgtgccctgg ccgcgcatgg gcccgagtgc ctgaccagtt ctgtcgccga gtcgatgacc
     1021 aaagacgtcg tgacctgcca ggaaaccgac accttggatg agttgatggc gatgatgacc
     1081 gcacgccgct tccggcattt gccggtgcag acggatggcg ccttggtcgg catcatttcc
     1141 attggcgacg tcgtcaagca tcacgtggca gaggtggaga tggaagccac ggccatgcgt
     1201 gattacatcg cccacagcta agccggtacg gcggttgcta gccagagatt gcgccgccgc
     1261 gggggctcag gcggagcgcg ccattgcgcg cggcggccga cgcaaggcga gctcgcgctc
     1321 gatgtcttcc agctcgcgtt gggccgcgag cttgccgccc ctgatcaagg catcggcctg
     1381 atgaaaatcg aacaggccga actgaggcaa cttcggcgaa atcacgatgt cgggtgggtc
     1441 accggcgagc cgcgagcggg caatgcggtc ttgcacgatg ttgaaggcct ccatcatcac
     1501 ccgcgaaatg ccgggcccac catcgccacg gccgaagaat tgccgcttca aaagtttgcg
     1561 catgccgccg acgccgtagg tcgcggccgg ttcgacggcc gacacgggtg cgtcctcggc
     1621 ggcggtgagc atttccatct ggggcacgac ggtgccgtgc ccgcacgaat cggaattgac
     1681 gttgacggcg atgacgtagc gcgctccgag cgcgcggcag accgagac
//
LOCUS       DASTMT010000496         3779 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_6379308, whole genome shotgun sequence.
ACCESSION   DASTMT010000496 DASTMT010000000
VERSION     DASTMT010000496.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3779)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3779)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3779
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6379308"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..756
                     /locus_tag="VFR00_11365"
     CDS             <1..756
                     /locus_tag="VFR00_11365"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011662340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU0159909.1"
                     /translation="VAEGAKVVFTGRRTAEGAAVAARIGSGAEFVAADATCEDDWRRV
                     IDHTLAAFGRIDCLFNNAGGPAPTGSIASVPVAGFDAAMALLVRSVMLGMKHVAPIML
                     RQRAGSIINNGSIAAHQTGRSTSMIYGAAKAAVNHLTKCVAMELGEHNVRVNSVSPGA
                     IATGILPKALGLEASKADALADAMKEVFAKAQPIPRAGVPDDIAQCVLWLASDRSTFV
                     NAVDIVVDGGLIGGAMFTPHHEGLKQLKARFGI"
     gene            785..1228
                     /locus_tag="VFR00_11370"
     CDS             785..1228
                     /locus_tag="VFR00_11370"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEU0159910.1"
                     /translation="MPLPRTPLLSVDCVVIDARGWVLLVRRGHPPFKGRHALPGGFVE
                     LGESVEAACRRELAEETGVRAGPLRLVGVYSDPGRDPRGHTCSVAFLTRVGRAKARAG
                     DDAAAAEWIEDWSRLPLAFDHAKILGDARRLLAQARRGAKAPGRA"
     gene            1263..2057
                     /locus_tag="VFR00_11375"
     CDS             1263..2057
                     /locus_tag="VFR00_11375"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012111536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU0159911.1"
                     /translation="MADFDGKVILITGSATGLGAALAIGVAERGAAAVILNCTKSLKE
                     AEATAAAVAEAGAVAVLAQGDVAEDSDCRRIAEAAARFGRLDALVNNAGTSKHVPNHA
                     DLDGLSKEDFLRIFAVNTVGPFQMVRACRSLLEASPSANVLMTSSIAGVAGGGSSVAY
                     AASKGALNTMTLSLARALAPKIRVNAICPGFIDTRWFRQGIGEEKATELRDRVARNSP
                     LQRAGAPEDIIGPAMFLISDAAHHITGETLMVDAGVHLNFAPLRAR"
     gene            complement(2068..2340)
                     /locus_tag="VFR00_11380"
     CDS             complement(2068..2340)
                     /locus_tag="VFR00_11380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019857211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3303 family protein"
                     /protein_id="HEU0159912.1"
                     /translation="MQFMVIETFRQQDAKAVYRRLRDKGRALPEGVVFISSYVSADLG
                     RCFQLMEAGEVTALQRWVAEWSDLAHFEIVPVVAGSDTAAALAGTL"
     gene            2442..2840
                     /locus_tag="VFR00_11385"
     CDS             2442..2840
                     /locus_tag="VFR00_11385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019172201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0159913.1"
                     /translation="MSAHSSLFLRRALMLDALASGATALLLIAAAPFLDALLGLPVAL
                     LRGAGLLLVPYVAFVAFVATRQRLEPAAVWVIVACNVLWVLASFLVLLSGKLAPTGLG
                     IAFVVAQAIVVALLGELQVMGLRRPAAEAA"
     gene            complement(2837..3520)
                     /gene="msrQ"
                     /locus_tag="VFR00_11390"
     CDS             complement(2837..3520)
                     /gene="msrQ"
                     /locus_tag="VFR00_11390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009763976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HEU0159914.1"
                     /translation="MPAPRRTRSTLATFLVGRDPIRRSIYLVGMLPAVFTFYLGLVDQ
                     LGADPQKTLERTLGLWALRFLILTLAITPLRRLGLANLVRYRRAIGLLAFYYAALHLT
                     VYTVLDQGLDLAAIWADIVKRPYITIGMLAFSILLPLAVTSNSPMIRRLGGAAWQRLH
                     RLVYLAAAAAVLHFIMLVKSWSPELILYAALIASLLAFRLLTRRPRTAAKRASAPAPQ
                     AARAHNLPP"
     gene            complement(3522..>3779)
                     /locus_tag="VFR00_11395"
     CDS             complement(3522..>3779)
                     /locus_tag="VFR00_11395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019443684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mononuclear molybdenum enzyme YedY"
                     /protein_id="HEU0159915.1"
                     /translation="GVKSIVKISLTEKQPPCTWNVQNAREYGFYSNVNPNVDHPRWSQ
                     ATERRIGEGGLFVKRRPTLMFNGYADEVASLYTGLDLKANY"
BASE COUNT          612 a         1228 c         1295 g          644 t
ORIGIN      
        1 gtggcggagg gcgccaaggt cgtcttcacg gggcgccgca cggccgaggg ggcggccgtc
       61 gccgcccgca tcggcagcgg cgccgaattc gtcgctgccg atgcaacctg cgaggacgat
      121 tggcgccgcg tgatcgacca cacgctggcc gcgttcggcc gcatcgattg cctgttcaac
      181 aatgcgggcg gccccgcccc gacgggaagc atcgccagcg tgcccgttgc cggcttcgat
      241 gccgccatgg cgctgttggt gcgctcggtg atgttgggca tgaagcacgt ggcgccgatc
      301 atgctgcggc agcgcgccgg cagcatcatt aacaatggct cgatcgcggc gcaccaaacg
      361 ggacgctcca cgtccatgat ctacggcgcg gcgaaagcgg ccgtgaacca tctgacgaag
      421 tgcgtggcca tggaattggg cgaacacaac gtgcgcgtca actcggtgtc gccgggggcc
      481 atcgccacgg gcatcttgcc caaggcgctg ggcctcgagg cgagcaaggc cgacgcgctc
      541 gccgacgcca tgaaggaggt gttcgccaag gcgcagccca ttccgcgcgc cggcgtgccg
      601 gacgacatcg cccagtgcgt gttgtggctg gcttcggatc ggtcgacctt cgtcaatgcc
      661 gtcgacatcg tggtcgatgg cggcttgatc ggcggcgcca tgttcacccc acaccatgag
      721 ggtctgaagc aattgaaggc caggttcggc atctagcctt gctgggaagg cgtgaggcat
      781 ccccatgccg ttgccgcgta caccgttgct cagcgttgat tgtgttgtta tcgacgcgag
      841 gggctgggtg ctgctggtgc gccgggggca tccccccttc aagggccggc acgccttgcc
      901 cggcgggttt gtcgagcttg gcgaaagcgt cgaggcggcc tgccggcgcg agctcgccga
      961 agagacgggc gtccgcgccg gacccttgcg cctggtcggc gtctactccg atccgggtcg
     1021 cgacccgcgt gggcatacct gctcggtcgc cttcctgacc cgcgtcggcc gcgccaaggc
     1081 aagggccggc gacgatgccg cggcggccga atggatcgag gactggtcgc gattgccgct
     1141 cgccttcgat cacgccaaga tattggggga tgcgcggcgc ctgctcgccc aggccaggcg
     1201 aggtgcaaag gcgcccggcc gcgcttaggc taggccgcgg ttagaaaaga aagaggcttc
     1261 gcatggcaga tttcgacggc aaggtgatct tgatcacggg ctcggccacg gggctgggcg
     1321 cggcgctggc gatcggcgtg gctgagcgcg gtgcggcggc ggtgatcctc aactgcacga
     1381 agagcctgaa ggaagcggaa gcgaccgctg ccgccgtggc cgaggccggc gccgtcgccg
     1441 tgttggcgca gggggatgtc gccgaggata gcgactgtcg tagaattgcc gaggcggccg
     1501 cccggttcgg caggctcgac gcgctcgtca acaatgccgg caccagcaag cacgtgccca
     1561 accatgccga tttggacggc ctctccaagg aggacttcct gcgcattttt gcggtcaaca
     1621 cggttggacc gttccagatg gttcgcgcct gtcgcagcct gctggaagct tcccccagtg
     1681 ccaacgttct gatgacctcg tcgatcgccg gggtcgccgg cggcggctcc tcggtggcct
     1741 acgcggcctc caagggcgcg ctcaacacca tgacgctatc gctcgcccgg gcgctcgcgc
     1801 ccaaaatccg ggtcaacgcc atctgccccg gtttcatcga cacgcgctgg ttccggcaag
     1861 gcattggcga ggaaaaagcc accgagctgc gcgaccgggt cgccaggaat tcgccgttgc
     1921 agcgtgccgg cgcccccgag gacatcatcg gcccggcgat gttcttaatc tcggatgccg
     1981 cccatcacat caccggcgag acactgatgg tcgatgctgg cgtgcacttg aactttgcgc
     2041 cgctgcgcgc gcgctaacct gccgccttta aagcgtgcca gcgagcgccg cggcggtgtc
     2101 gctgcccgcc accaccggca cgatctcgaa gtgcgcgagg tccgaccatt ccgcgaccca
     2161 gcgctgcagc gccgtaacct cgccggcttc catgagctgg aagcagcggc cgaggtcggc
     2221 gctcacatag ctgctgataa aaacgacgcc ttcgggcagc gcccgaccct tgtcgcgcaa
     2281 gcgccgatag acggccttgg catcctgctg gcggaatgtc tcgatcacca tgaactgcat
     2341 tgcgacccct caaaaaagca acatcgctta cccctgacgt aatcgacttc gcctcctgtg
     2401 cgctcgatca tagccgcaca aacgaaggaa aggagattgc catgtcagcc cattcatcgc
     2461 tttttctgcg ccgcgccctg atgctcgacg cactcgccag cggcgcaacc gcgcttttgc
     2521 tcattgccgc cgcccccttc ctcgacgcgc tcttgggcct gcccgtcgcc cttctgcgcg
     2581 gggcgggtct cctgctcgtg ccttacgtgg ccttcgttgc ctttgtggcg acgcggcagc
     2641 gcctcgagcc ggcggcggtc tgggtcatcg tcgcctgcaa cgtcttgtgg gtgctcgcca
     2701 gcttcctggt gttgttgagc ggcaagctcg cgccgacggg gctcggcatc gcctttgtgg
     2761 tggcgcaggc gatcgtggtg gcgctcttgg gcgagctgca agtgatgggc ctgcggcggc
     2821 ccgctgccga ggccgcctaa ggtggcaggt tgtgagcgcg cgctgcctgc ggggcgggcg
     2881 cgcttgccct cttcgcggcc gtgcgtggcc ggcgcgtcaa cagccggaag gcaagcaggc
     2941 tcgcgatcag ggcggcatag aggatgagct cgggggacca ggatttcacg agcatgatga
     3001 aatgcaggac tgcggcggcc gcggccaggt aaacgagccg atgcagccgc tgccaggcgg
     3061 cgccgccgag ccggcggatc attggcgagt tcgacgtgac cgccaacggc agcaggatgc
     3121 tgaaggccaa catgccgatc gtgatatagg gccgcttgac gatgtcggcc cagatggcgg
     3181 ccagatcgag gccctggtcg agcacggtgt agaccgtcag gtgcaaggcc gcatagtaaa
     3241 aggcaagcag gccgatagcc cgccggtagc gcaccagatt ggcgagaccg agccgacgca
     3301 gcggggtgat ggccagcgtg aggatcaaga agcgcagcgc ccacagcccg agcgtccgct
     3361 ccagtgtttt ctgcggatcg gcgccgagct gatcgacgag ccccaagtag aaagtaaaga
     3421 cggcgggcag catgccgacg agatagatgc tgcgccggat cgggtcgcgt ccgaccagga
     3481 aggtggccag cgtcgagcgc gtccgcctcg gcgccggcat cctagtagtt cgccttgagg
     3541 tcgagccccg tgtagaggct cgccacctcg tcggcgtatc cattgaacat cagcgtcggc
     3601 cgccgcttca cgaacaagcc accctcgccg atgcggcgct cggtggcttg gctccagcgc
     3661 gggtggtcga cgttgggatt gacgttggaa taaaagccgt actcacgcgc gttttgcacg
     3721 ttccaggtgc acggcggctg tttttcggta aggctgattt tgacgatcga cttcacgcc
//
LOCUS       DASTMT010000497         6336 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_2574440, whole genome shotgun sequence.
ACCESSION   DASTMT010000497 DASTMT010000000
VERSION     DASTMT010000497.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 6336)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6336)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6336
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2574440"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1254
                     /locus_tag="VFR00_11400"
     CDS             <1..1254
                     /locus_tag="VFR00_11400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019198738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HEU0159916.1"
                     /translation="DQIGQLIGRLSARALIVEPGWGSAPAAPEAKAANLPSLKLLYTP
                     EQLPSQGSSSTAPSTDPDRVLYLAFTSGTTGTPKCVMHSHNTLLSNARDLVADWGHGP
                     ETVLLSLSPLSHHIAWVGVAQWLIAGCQFVCHDPPAGSSTLDWLLATGATYVMGVPTH
                     AMDILAEQRRQGLSRLGRVSVFYMAGAPIPPSLAGAFVAQGITPQNVYGMTENSSHQY
                     THPADAADTIVSTCGRGGAGYTVKLFDPANRDEPAKPGEMGEIGGRGPALMLGYFDNQ
                     GATEASFNRHGWFMSGDLGSMDADGNLKIEGRLKDVIIRGGHNIYPAQIEALALRHPE
                     AGRVACFPVADPRLGERVCLAVSGSAAAQDLIEHLGRQGLSKYDMPEYFLRLSEFPLT
                     ASGKILKRELVEMVKRGELLPEPVR"
     gene            1281..2048
                     /locus_tag="VFR00_11405"
     CDS             1281..2048
                     /locus_tag="VFR00_11405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019198733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEU0159917.1"
                     /translation="MSVELTTRETCAVLMLNRPEALNALSVAVLRQIDEALETVAKSR
                     ARALIITGAGNKAFCAGADIKEFRHQAMLDSKARTELGQATFAKLDRLPIASIAVING
                     YAFGGGLELALAATFRLALPNAKLGQPEIKLGTIPGYGGTQRLPRLIGEARALELILT
                     GRTVEAAEAERIGLVNRLVEGDAVEAGLAFAREFTGYSLPVLALARAAVQRALDLPIH
                     EGLKVEADLSTLSFQTRDADEGAAAFTEKRKARFVDA"
     gene            2041..2748
                     /locus_tag="VFR00_11410"
     CDS             2041..2748
                     /locus_tag="VFR00_11410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011582263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU0159918.1"
                     /translation="MPEGAVLVTGANRGIGAAIAAELAAAGRRVAGLTRSGCSAAGEG
                     FVCDMSEENSIAQAVAAVAAHGPIAGLVNNAGLYEAHVSAELTAADFERVMRVNATAV
                     VVAARQVYPHLKRAGGGLIVNIGSYYDKLGVTNTVAYCACKAAVGAITRCLAVEWAPD
                     NIRVIDVAPGYIGTELNAKFRAREATQRWIKQQIPVGRAGTPAEIGKLVALLFGADIA
                     YLTGETIYVDGAAGMRH"
     gene            2816..3772
                     /locus_tag="VFR00_11415"
     CDS             2816..3772
                     /locus_tag="VFR00_11415"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010909834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEU0159919.1"
                     /translation="MPRHQSYLPHGVIPAVLLPFDAELAIDEASFRKHLRDVAATPGI
                     TGLTINAHSTEVASCTFDEQRRVLEIAQDEIGDRLPLINGIWADGSLEAARLARMAEA
                     GGASALLVFPPAPFTLGQSPAMAIAHFKRIAEATELPLIAFQYPLATGQGYPPDTLLK
                     MCEEVPSIRAIKDWIGNVAAHEWHVRTLQNLARPVNVLSTHSAWLFASLVLGCNGLLS
                     GSGSVIPELQSALFKAVRENDLAQAKQLNDRIEPLMRVFYADPFVDMHNRMKEALVLL
                     GRIPRAYVRPPLVKIQRPEIARIRQGLIAAGLLAKDAGRDAA"
     gene            3849..4514
                     /locus_tag="VFR00_11420"
     CDS             3849..4514
                     /locus_tag="VFR00_11420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0159920.1"
                     /translation="MPGILAVFNDCAPEGHQHFETWYNREHLQERVAVPGFRFGRRYE
                     LVAGGDRQFCAFYEVDTAAVLNSPFYIGRLNDPTPWTQAAMPLFRNMVRTVCDVTAAA
                     GDLIGACAVVLRADGAMAPTSAAPAWVETLAGEAGIARVELWTQAAQQTNPDTAEMKS
                     RGKDRLAAGVVLVECVRRTDAERIMRVLRDPPAALGVTGEMALGIYQLLCLYQPGRSA
                     HRK"
     gene            complement(4574..6118)
                     /locus_tag="VFR00_11425"
     CDS             complement(4574..6118)
                     /locus_tag="VFR00_11425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007615720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate-CoA ligase family protein"
                     /protein_id="HEU0159921.1"
                     /translation="MSYPRIYNAAVDMVDRNVAAGRGEKTVFIDPLEQVSYGELKART
                     NRMANLLATYGVARESRVALLLLDTVDFPVAFWGAIKAGVVPVALNTLLTTEQYAYIL
                     ADSRVKALFVSAPLLAAVQPTLGQLPFLKHIFVCGGEPPPYALSFRAELDLQAASFEA
                     AETCCDETAFWLYSSGSTGMPKGVRHVHSSPMETARLCGQQCLGMRADDVVYSAAKLF
                     FAYGLGNAMSFPMSVGASAVLSPDRPTPELVFRTLKQQQPTFFFGVPTLYAAMLAYPE
                     GTPEMSSGRLRVCVSAGEALPAEVGRAFRAKFGVDVYDGVGSTEMLHQYVCNSAAGLK
                     YGTSGKPVPGYEIRLVDEHGQEVGENVVGEMLVRGATHSEGYWNQREKSRATFEGGWT
                     RTGDKYTRDGDGFFTYQGRTDDMFKVSGIWVSPFEVESALIGHAAVMEAAVVPKADSD
                     GLLKPKAFIVLKPGKPAGNGLEEELKEQVKQKAGPWKYPRWIEVVEALPKTATGKIQR
                     FKLREA"
BASE COUNT         1084 a         2113 c         2053 g         1086 t
ORIGIN      
        1 gatcagatcg gccagcttat tggccgcttg agcgcgcgcg ccctgatcgt ggagccgggc
       61 tggggatccg cacccgccgc gcccgaggct aaggcggcga accttccctc cctcaagctc
      121 ctctacacgc cggagcagct gccgagccag ggcagcagca gcactgcgcc cagcaccgat
      181 cccgatcgcg tcctctatct cgccttcacg tcgggcacga cgggcacgcc caagtgcgtc
      241 atgcactcgc acaacacgct actcagcaat gctcgggatt tggtggccga ctggggtcat
      301 ggacccgaga ccgtgctgct cagcttgtcg ccactatcgc atcacattgc ctgggtcggg
      361 gtggcgcaat ggttgatcgc ggggtgccaa ttcgtctgcc atgacccgcc ggcgggcagc
      421 tccacgcttg attggctgct ggcgaccggc gccacctacg tcatgggcgt acccacgcat
      481 gcgatggaca ttttggccga gcagcggcga cagggcctgt cgcggctcgg gcgagtgtcg
      541 gtattctaca tggcgggggc gcctattccg ccgtccctgg cgggcgcctt cgtggcccag
      601 ggcatcacgc cgcagaacgt ctacggcatg accgagaact cctcgcatca gtatacgcat
      661 cccgccgacg ccgccgacac catcgtttcc acgtgcggcc gcggcggcgc cggctacacg
      721 gtgaagttgt ttgaccccgc caatagggat gagcccgcca agcctggtga aatgggtgag
      781 atcggcggcc gtggacccgc ccttatgctc ggctattttg acaaccaggg cgccaccgag
      841 gccagtttca atcggcatgg atggttcatg tcgggcgatc tcggcagcat ggatgcggac
      901 ggcaacctga aaatcgaggg ccgtctcaag gacgttatta ttcgcggcgg ccataacatc
      961 tatcccgcgc agatcgaggc actcgccctg cgccatcccg aggccggccg ggtcgcctgc
     1021 tttcccgtcg ccgacccacg gctcggcgag cgggtgtgtc ttgccgtgtc gggcagcgcc
     1081 gcggcgcagg atctgatcga gcatcttggc cggcaggggc tctcgaagta cgacatgccc
     1141 gagtatttcc tccggctcag cgaattcccg ttgaccgcca gcggcaagat tctcaagcgt
     1201 gagctggtcg aaatggtaaa gcgcggggag cttttgcccg agcccgtccg ttgacccaag
     1261 ccgagaaagg ggaacgaccg atgtctgtcg agctgacaac gcgcgagacc tgcgccgtgc
     1321 tgatgctcaa tcgacccgaa gcgctcaacg cgctctccgt ggcggttttg cgccaaatcg
     1381 acgaggcgtt ggagacggta gcaaaatcga gggcgcgggc gctcatcatc accggggcgg
     1441 gcaacaaggc attctgcgcc ggcgccgaca tcaaggagtt ccgccaccag gccatgctgg
     1501 acagcaaggc gcgcacggag ctcgggcaag ccactttcgc caagctggat cgattgccga
     1561 tcgcctcgat cgcggtgatc aatggttatg ccttcggcgg cggtctcgag ctggcgctcg
     1621 ccgcgacttt ccggctggcg ctgcccaacg ccaagctggg gcagcccgaa atcaagctcg
     1681 gcaccatccc cggctatggt ggcacgcagc ggctgccgcg gctgatcggc gaggcgcgcg
     1741 ccctggagct gattttgacc gggcgcacgg tggaggccgc ggaggcagag cgcatcgggc
     1801 tcgtcaatcg cctcgtcgag ggtgacgcgg tcgaagccgg gctcgccttc gcccgcgagt
     1861 tcaccggcta tagcttgccc gtgctggccc tcgcccgcgc ggccgtgcag cgcgcgctcg
     1921 acctgccgat ccacgaaggt ctgaaggtgg aagcggatct gtccaccctt tcattccaga
     1981 cccgggatgc cgacgagggc gccgccgcct tcacggagaa gcgcaaggcg aggtttgtcg
     2041 atgcctgaag gagcggttct cgtcaccggc gccaatcgcg gcatcggtgc tgccattgcc
     2101 gcggaacttg ccgccgccgg ccggcgcgtg gcggggctga cacgcagcgg ttgttcggcc
     2161 gcgggcgagg gtttcgtgtg cgacatgagc gaagagaact cgattgcaca ggcggtcgcc
     2221 gcggtggcgg cgcatggtcc cattgctggg ctcgtcaaca atgccggtct ctatgaagcc
     2281 cacgtcagtg ccgagctgac ggccgccgac ttcgaacgcg tcatgcgcgt caatgccacc
     2341 gcggtggtgg tggcggcacg ccaggtttat ccgcacctga agcgcgcggg cggtgggctg
     2401 atcgttaata tcggctccta ctatgacaag ctcggggtga ccaacaccgt ggcctactgc
     2461 gcctgcaagg ccgcggtcgg ggccatcacc cggtgtctgg ccgtggaatg ggcgcccgac
     2521 aatatccgcg tcatcgatgt ggcccccggc tatatcggca ccgaactcaa cgccaaattc
     2581 cgcgcccggg aggccacgca gcgctggatc aaacagcaaa ttcccgttgg ccgcgccggc
     2641 acgcccgcgg aaatcggcaa gctcgtggcc ctgctcttcg gcgctgacat cgcctatctg
     2701 accggcgaaa cgatttatgt cgacggggcc gcggggatgc ggcattgacc ctcccccgtc
     2761 ccttgacccg agccggcggc gcccgcacag tggcgccaaa ctgtggagac ggaacatgcc
     2821 ccgccatcaa tcctaccttc cgcatggcgt gatcccagcg gtgttgctgc cgttcgatgc
     2881 cgagctcgcc atcgacgagg cgagtttccg caagcatctg cgcgacgtgg ccgccacccc
     2941 cggcattacc ggcctcacta ttaatgcgca ttcgaccgag gttgcttcct gcacattcga
     3001 cgaacagcgt cgcgtgctgg aaatcgcgca ggacgaaatc ggcgaccggc tgccgttaat
     3061 caacggcatc tgggccgacg gcagtctcga ggccgcccgg cttgcccgca tggccgaggc
     3121 gggtggggcc tccgcactct tggtatttcc gccggccccg ttcacgctcg gccagtcgcc
     3181 cgccatggcg atcgcgcatt tcaagcgcat tgccgaggca accgagctgc cgctcattgc
     3241 cttccaatat cccctcgcca ccggccaggg ctatccgccc gataccctct tgaaaatgtg
     3301 cgaagaggtg ccctcgatcc gagccatcaa ggattggatc ggcaacgttg ccgcgcacga
     3361 atggcacgtg cgcaccctgc aaaacttggc tcgccccgtg aacgtgctgt ccacgcacag
     3421 tgcctggctg ttcgcttcgc ttgtgctcgg ctgcaacggc ctgctctcgg gcagcggcag
     3481 cgtcatcccc gagctgcaga gcgccttgtt caaggccgtc agggaaaacg atctggcgca
     3541 ggccaagcaa ctgaacgacc gcatcgagcc gctgatgcgc gtcttttatg ccgacccgtt
     3601 cgtcgacatg cacaaccgca tgaaggaggc cctggtgctg ctcggccgta tcccgcgcgc
     3661 ctacgtgcgg ccgccgttgg tgaaaattca gcggcccgag atcgcccgta tccgtcaggg
     3721 tttgatcgcg gccggcctgc tggcaaagga cgccggccgc gacgccgcct gagcgggcga
     3781 cgtcttgccg cctgaacctg ccaccccgag gctagccctc ctgaaacata gatctaatga
     3841 gttggtcgat gcctggcatt ctcgcggtct ttaacgattg cgcgcccgag gggcatcaac
     3901 acttcgagac ctggtacaac cgcgagcacc tgcaggagcg cgttgccgtt ccgggctttc
     3961 gtttcggccg gcgctacgag ctggtcgccg gcggcgaccg gcaattctgt gcgttttacg
     4021 aggtggacac ggcagccgtt ctcaattcgc cgttctatat cggccggctc aacgatccga
     4081 cgccgtggac gcaagcggcc atgccactct tccgcaacat ggtgcgcacg gtttgcgacg
     4141 taacggcggc ggcgggtgac ctgatcggcg cctgcgccgt cgttctgcgc gccgatgggg
     4201 cgatggcgcc gacgtcggca gccccggcct gggtcgagac gctggcaggc gaggcgggca
     4261 tcgcgcgtgt cgagctctgg acccaggcgg cacagcagac caaccccgac acggcagaga
     4321 tgaagagccg cggcaaggat cggctggccg ccggtgtcgt actggtcgag tgcgtacgcc
     4381 gcaccgatgc agagcggatc atgcgcgtct tgcgggatcc cccggcggcg ctcggcgtga
     4441 ccggggagat ggcgctcggc atttaccagc tgctctgcct ctaccagccg gggcgctcag
     4501 cgcatcggaa gtagccgctg acgggccgcc ccacgcgcct tcagacccct cgcccggcag
     4561 gtataagagt gcctcaggcc tcgcgcagct tgaagcgctg gatttttccg gtggcggtct
     4621 tgggcagcgc ctcgacgact tcgatccagc gcgggtactt ccacggaccc gccttctgct
     4681 tgacctgctc cttcagctcc tcctcgagcc cgttgcccgc gggcttgccg ggcttcagca
     4741 cgataaaggc cttcggtttc aacagcccat cgctgtcagc cttcggcacc acggcggctt
     4801 ccatcaccgc ggcatggccg atcagcgcgc tttccacctc aaagggggac acccagatcc
     4861 cggaaacctt gaacatgtca tcggtgcgcc cctgataggt gaaaaaacca tcgccatcgc
     4921 gggtgtactt gtcgcccgtg cgcgtccaac caccctcgaa ggtggcgcga cttttctcgc
     4981 gctggttcca atagccctcc gaatgggtgg cgccgcgtac gagcatttct cccaccacgt
     5041 tttcgcccac ctcctggccg tgctcgtcga caaggcggat ctcataaccc gggacgggct
     5101 tgccggaggt cccgtacttg aggccggccg cgctgttgca gacatattgg tgcaacattt
     5161 cggtggagcc gacgccgtca taaacgtcga cgccaaactt ggcgcggaat gcccgtccca
     5221 cctcggcggg cagggcctcg ccggccgaga cgcacacgcg caagcgcccg gagctcatct
     5281 cgggtgtgcc ctccggatag gccagcatgg cggcataaag ggtcggcacg ccgaagaaga
     5341 aggtcggttg ctgctgcttg agcgtgcgaa acaccagttc gggcgttggc cgatcgggcg
     5401 acaacaccgc gctggcgccg accgacatcg ggaacgacat ggcattaccc agcccatagg
     5461 cgaagaacag cttggcggcc gagtagacga cgtcgtccgc ccgcatgccg aggcactgct
     5521 ggccgcacag gcgcgcggtt tccatcgggc tcgagtgcac gtggcgaacg cccttcggca
     5581 tgccggtgga acccgaggaa taaagccaga aggcggtctc atcacagcag gtctcggccg
     5641 cctcgaagct ggccgcctgc aaatcgagct cggctcggaa cgacagcgca tagggcggcg
     5701 gctcgccgcc gcaaacgaag atgtgcttga ggaaggggag ctgacccagt gttggctgca
     5761 ccgcggcaag caggggggcc gaaacaaaca gcgctttgac gcggctgtcg gcaagaatgt
     5821 aggcgtattg ttccgtggtg agcagcgtat tgagcgccac cggcacgacg cccgccttga
     5881 tggcgcccca gaaggcgacg ggaaagtcga cggtgtcgag caagagcagc gccacgcgcg
     5941 actcccgggc cacgccgtag gtggccagca ggttggccat ccggttggtg cgcgccttga
     6001 gctcgccgta gctcacctgc tcaaggggat cgatgaaaac cgtcttctcg ccacgccccg
     6061 ccgcgacgtt gcggtcaacc atgtcgacgg cggcgttgta aatacgcgga tagctcattg
     6121 ctcctccccg tcggcgcctg gcctgccccg actttgcccc cgccagggat ggcgggcgtt
     6181 ttctgcatca tactgcaaag tcgggcgggg atgcaggggg ttgcttgccc tcggtcggcg
     6241 acggaagatg tagtatactg cagaaagacg ccccagcggg ccgcggcgac cgacgagacg
     6301 atgccaacag cacccgagat tggcccggaa gggaga
//
LOCUS       DASTMT010000498         2741 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_4737868, whole genome shotgun sequence.
ACCESSION   DASTMT010000498 DASTMT010000000
VERSION     DASTMT010000498.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2741)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2741)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2741
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4737868"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1257)
                     /locus_tag="VFR00_11430"
     CDS             complement(<1..1257)
                     /locus_tag="VFR00_11430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015688961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEU0159922.1"
                     /translation="MQQRVLHTAKSACPHDCPSACALEVNVLEDGRIGRITGARHNSY
                     TLGVVCAKVARYHERIDHPERLLHPLRRIGPKGSGQFERISWPAALAEIAERFDAIAK
                     RWGPEAIWPYHSGGNMGVLQRYGLDRLRNPMRYSRQHTTICVTPAESGWLAGVGKLVG
                     PDPREMEDADLIVIWGGNPVSTQVNVMTHVARARKARGTKLIVVDVYRTPTVERADIA
                     LIVKPGSDGALALAVMNVLLTEGLLDRDYLGRYTDFDAEVERHIRTRTPQWAAAITGL
                     SVDEIVAFARLYGATRKSFIRVGFGFTRSRNGASAMHAVSCLPALTGAWSRRGGGAFF
                     LSFDKQQWGLDTSLINASDAIDLSVRILDQSRIGAVLCGEPEALGAGPPVEAILMQNA
                     NSATVAPSTAKVLKGLSREDLFVVVQE"
     gene            complement(1396..1857)
                     /locus_tag="VFR00_11435"
     CDS             complement(1396..1857)
                     /locus_tag="VFR00_11435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEU0159923.1"
                     /translation="MVSHVVLIRALQRYWRWRRGLTMGAQALVLDAQGRVLLVRHGYQ
                     PGWHFPGGGVEKGESVLSAMARELREEVGITLAGPAQLFGVYANFAAFPGDHIAFFIV
                     RSWQQASVPAPNREIREQRFFATDQLPPGTTRATRRRLAEVLGGAEQSQSW"
     gene            1961..>2741
                     /locus_tag="VFR00_11440"
     CDS             1961..>2741
                     /locus_tag="VFR00_11440"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEU0159924.1"
                     /translation="MQTALTIAHLSDVHLGPIRGFTPRYWNLKRLTGYWNWRSTRRGQ
                     PQRGVLDRLVADVQAQRPDHVVVTGDLANIGLPQEHVDALTWLKALGSPDRVSVIPGN
                     HDIYARGGRDLGTRRWADYMRSNAEGAPLLGAAADAGAFPFVRRLDGVALIGLNSAVP
                     TPPLIAAGRLGSAQLARLADVLERAKRQGLFRLVLIHHPPLPGQASAGRGLRDAAALQ
                     AILVAQGAELVIHGHNHRDMLALQASASGASIPIVGVASASL"
BASE COUNT          448 a          980 c          875 g          438 t
ORIGIN      
        1 ctcctgcacc accacgaaca ggtcctcgcg gctgagcccc ttgaggacct tggccgtgct
       61 gggcgccacc gttgccgagt tggcgttctg catcagtatg gcctcgaccg gcggcccggc
      121 cccgagcgcc tcgggctcgc cgcacagcac cgcaccaatc cgggattggt cgaggatcct
      181 gaccgagaga tcgatggcgt cgctggcatt gatgagcgag gtgtcgagcc cccactgctg
      241 cttgtcgaag ctgaggaaga aggcgccacc accccgccgc gaccaggcgc cggtcagcgc
      301 cggcaggcaa gacacggcat gcatggccga agcaccgttg cggctgcgcg taaagccaaa
      361 tccgacccgg atgaagctct tgcgcgttgc gccatataag cgcgcgaagg cgacgatctc
      421 gtcgacgctg agccccgtga ttgcggcggc ccattgcggc gtgcgcgtgc gaatgtgacg
      481 ttcgacctcg gcgtcgaaat ccgtgtagcg gccgaggtag tcgcgatcga gcagcccctc
      541 cgtgagaagc acgttcatga cggccagtgc cagggcgcca tcggagcccg gcttgacgat
      601 caaggcgatg tcggcccgct cgacagtcgg cgtacgatag acatcgacga cgatcagctt
      661 ggtgccccgc gccttacgcg cgcgggccac atgcgtcatg acgttgacct gggtggagac
      721 ggggttgcct ccccagatca cgatcaagtc cgcgtcctcc atttcgcgcg gatccggtcc
      781 caccagcttg ccgacaccgg ccagccatcc cgattcggcc ggcgtcacgc agatggtcgt
      841 gtgctggcgc gagtagcgca tcgggttgcg caggcgatcg agaccgtagc gctgcagcac
      901 gcccatgttg ccgccggaat gataaggcca gatcgcctcc ggtccccaac gcttggcgat
      961 ggcgtcgaag cgctcggcga tctccgccag cgccgccggc caggagatgc gctcgaactg
     1021 cccggagccc ttcggcccga tgcgacgcaa gggatggagc agacgctcgg ggtggtcgat
     1081 gcgctcgtgg tagcgcgcaa ccttggcaca caccacgccc agcgtgtagc tattgtgccg
     1141 ggcgcccgtg atccgcccga tgcgaccatc ctccagcaca ttgacctcga gcgcgcaggc
     1201 gctcgggcag tcgtgcggac aggctgattt ggccgtgtga agaacgcgtt gttgcatacg
     1261 acatcctgcg ggcgacgctt gcaaggccga gttcggccga tcctaggcga aaaatcgcct
     1321 cggggcgaat aggctttgcc ccaaccggcg cggtcgcagc cccagcgcgg gccagcgacg
     1381 tgggtggcag cgcgctcacc aggactggct ctgttcggct ccgcccaaaa cttccgccag
     1441 ccggcgccgg gtcgcccgcg tcgtgccggg cggcaattga tccgtggcaa aaaagcgctg
     1501 ttcgcggatt tcgcggttcg gcgcaggcac gctcgcctgc tgccagctgc gcacaatgaa
     1561 gaaggcgata tggtcgccag ggaaggccgc gaaattggca taaacgccaa acagctgcgc
     1621 gggtcccgcc agcgtgatgc ccacctcctc gcgcagctca cgcgccatcg cgctcagcac
     1681 cgattcgccc ttctccacac cgccgccggg aaagtgccag cccggctggt agccatgccg
     1741 caccaagagc acgcgccctt gcgcgtccag cacgagggct tgcgccccca tggtgagccc
     1801 ccggcgccag cgccagtagc gctgcagggc ccttatcaaa acgacatggc tgaccatcgg
     1861 ctatcgctgc gcgtctttac acctgcacgc tcgccctgtc ttcgaccggc cgcatcgatt
     1921 tggcaaggct cggcgaagcg gctaccaatc caactcaccg atgcagaccg cgctcaccat
     1981 cgcccatctc tccgacgtcc acctcggccc catccgcggc ttcacgccac gctactggaa
     2041 cctgaagcga ttgaccggct attggaactg gcgcagcacg cgccggggcc agcctcagcg
     2101 cggcgtgctc gacaggctgg ttgccgacgt gcaggcgcag cgaccggacc acgtcgtcgt
     2161 cacgggcgat cttgccaaca tcggcctgcc gcaggagcat gtcgacgcct tgacgtggct
     2221 aaaagccctc ggcagcccgg accgcgtcag cgtcattccg ggcaaccatg acatctatgc
     2281 gcgcggcggt cgcgatctcg gcacgcggcg ctgggccgac tacatgcgtt ccaatgccga
     2341 gggcgcaccg ctgttgggtg ccgccgccga cgccggcgcc tttcccttcg ttcggcgcct
     2401 cgacggcgtc gccctcatcg ggctcaattc ggccgtgccg acgccgccgc tgattgccgc
     2461 cggccgcctc ggcagcgcgc aattggcccg cctggcagac gtgcttgagc gcgccaagcg
     2521 ccaaggcctg ttccgcctgg tgctcatcca ccatccgccg ctgcccggcc aggcctcggc
     2581 cggccgcggc ttaagggatg ccgcggccct gcaggcgatc cttgttgcgc agggcgccga
     2641 actcgtcatt cacggccaca accatcgcga tatgctggcc ctgcaagcct ccgcctcggg
     2701 cgcatcaatc ccgatcgttg gcgtcgcctc ggcctcgctt g
//
LOCUS       DASTMT010000499         3218 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_1595317, whole genome shotgun sequence.
ACCESSION   DASTMT010000499 DASTMT010000000
VERSION     DASTMT010000499.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3218)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3218)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3218
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1595317"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..472)
                     /locus_tag="VFR00_11445"
     CDS             complement(<1..472)
                     /locus_tag="VFR00_11445"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like serine protease"
                     /protein_id="HEU0159925.1"
                     /translation="MPITVPSYVVGCLVLVAALLFSVNPGGAQDCRTGTAARTKVVGG
                     EAAHLKNWPGLAVLRVNASAAKQALYLCGGAAINDRWVVTAAHCLEAVGAGSKTSFVD
                     ARGRSRDGVLEVVLGVDNLDLVSDKHVFEVEKVVVKEGYQNAAKTGNDLALIRLK"
     gene            672..1229
                     /locus_tag="VFR00_11450"
     CDS             672..1229
                     /locus_tag="VFR00_11450"
                     /inference="COORDINATES: protein motif:HMM:NF015669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HEU0159926.1"
                     /translation="MSKRRQFLPTALLLSISLASPAMAQAPETHWKLWRAKDIATATG
                     VMPQLTRKGEALSGSTGCNTFTTTLTEQGSGKVSIERPALTRKFCGGQQQVVENAFLD
                     ALGRTAFVQEAGERLVFLSAARQPLLIWRALRASASPRAQSNARAKQPSTTRRARVAK
                     LHKHARKTSPNRGAEKGCLFFMQWR"
     gene            complement(1275..1481)
                     /locus_tag="VFR00_11455"
     CDS             complement(1275..1481)
                     /locus_tag="VFR00_11455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0159927.1"
                     /translation="MLDVEVMLVVVALSGGAEELLKVPMPSLEHCQAYARLWNGRLPG
                     VGASCVVIERGVKLSRAFPEPRHG"
     gene            complement(1896..2819)
                     /locus_tag="VFR00_11460"
     CDS             complement(1896..2819)
                     /locus_tag="VFR00_11460"
                     /inference="COORDINATES: protein motif:HMM:NF039905.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEU0159928.1"
                     /translation="MAAPHSMRRRFLVLVNAGAGLSRPWLVDEVARALERAGASVTRE
                     TLREFAAIRRQAAAAARSRRYDVVVAAGGDGTIRAAAAGMNGTDVPLAIIPCGTANVL
                     AQEIGLKAKPDLVCQTLRHGPPISIACPRANGEPFLLMAGAGFDARVVGALNHALKSR
                     IGKVAYTGPVLGALMRPPDTLSVTIDNQCHEASWVVIANARHYAGRFVLAPGTGIQQR
                     GLEAVLFRARSRVVLMGQLLSLLRGQLDARAARNGDVVMHQCAHVKITASHPVPVQID
                     GDPFGVTPLEAQAGSEVLRLIVPAGAHGSVP"
     gene            complement(2895..>3218)
                     /locus_tag="VFR00_11465"
     CDS             complement(2895..>3218)
                     /locus_tag="VFR00_11465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HEU0159929.1"
                     /translation="EGGGEWQDWLVDDTPDQEERLAESEELTLRKGYLAKAMSTLNER
                     EKRIFEARRLAEEPATLEDLSAEFGVSRERIRQIEVRAFEKVQAAVQKSARRDEEGAT
                     SPAGA"
BASE COUNT          601 a         1081 c         1004 g          532 t
ORIGIN      
        1 tcttcaggcg aattagcgcc agatcgttgc cggttttggc cgcgttctga tagccctctt
       61 tgacgacgac tttttccacc tcgaagacgt gcttgtcgct gacaaggtcg agattatcca
      121 cacccagcac aacctccagc accccgtcgc gggaccgccc tcgcgcgtcg acgaaggagg
      181 tcttcgaacc ggcgcccact gcctccaagc aatgcgccgc ggtcaccacc caacggtcat
      241 tgatagcggc cccgccgcag agatagagcg cctgtttggc cgcgctcgcg ttcacgcgca
      301 aaaccgccaa gcctggccaa tttttgaggt gagcggcctc gccccccaca accttggtgc
      361 gtgctgccgt gccggtgcgg caatcctgcg cgccgcccgg gttgaccgag aagaggagag
      421 ccgccaccag cacaaggcag cccactacgt agctcggcac ggtgattggc atgaggcccc
      481 cttagcgacc aagagtttaa cccattgcgg cgaggtggcc aacgaagccc ggcgccttgt
      541 atggccgcgg gcagcggcgg cgcgggcgcc tcaaattcga tctattaagg ggaagggcgt
      601 agaaccatcg gacccccccg ggcctaagct ggcgtgggcg actgaagacg cggagcccga
      661 gcgagagcgc aatgtcgaaa cggcggcaat tcctccctac cgcacttttg ctatcgatct
      721 cacttgcatc gccggcgatg gctcaggcgc cagaaacgca ctggaaactc tggcgcgcca
      781 aggacatcgc gaccgccacc ggcgtcatgc cgcagctcac ccgcaaaggt gaggccctgt
      841 ccggttcgac cgggtgcaat acgtttacga ccacgctaac cgagcaaggc agcggcaagg
      901 tcagcatcga gcggccggcc ttgacgcgca agttctgtgg tggacagcaa caggtggtcg
      961 agaacgcatt cctcgatgca ctgggccgga ctgcgttcgt gcaggaagcc ggcgagcgat
     1021 tggtgttcct gtcggcggca cggcagccat tgttgatttg gcgagcgctg cgcgcgtcgg
     1081 cctcaccacg ggcgcaatcg aacgcgcgcg ccaaacagcc gagcacgacc aggcgcgctc
     1141 gtgtggcaaa actccacaag cacgcgcgga agacctcacc taatcgcggt gcagaaaaag
     1201 gctgcttgtt ctttatgcaa tggcgttgag aagcatcaag ttcgatgcgg tgccgatcag
     1261 acaaggctcg agcctcagcc gtggcgcggc tctggaaagg cgcggcttaa cttcacgccc
     1321 cgctcgatga cgacacagga cgcaccaacg cccggtagtc gcccattcca taatcgcgca
     1381 taggcttggc aatgctccag cgatggcatc ggcaccttca gcaactcttc ggcaccgccc
     1441 gaaagggcga cgaccacgag catgacctca acgtcgagca tgcgctcggc tccaacattc
     1501 gcattggtat tcgcccgcta cagccaagcc tatcccgcaa gcgttaaagc ggactgaacc
     1561 cggcttgcgc gcgtccgggg cacgatgggc cggggtcgct agctcgggta cagcaccatg
     1621 gccacggcaa gcagaattgc cagcgtggcc aacgacattc cggcgagcgc gccgcggccc
     1681 ttgggcggtc gtaaaatgct catgacggcc ttgcctcaca ccgcgcgtgc ctgaggcttg
     1741 gcgcatcact tcccctggcg gctaccacca aagagacggt cggctcccat tcgattactc
     1801 gctttggacc gtggattgag cgcgaggcag cgcttgcgtt cggtgggcca gcgaagcccg
     1861 gccgcgggcg tcttcgccaa gccggtgggg gacgtctagg gtacgctgcc gtgtgcgccg
     1921 gccgggacaa tcagcctgag cacttcggag ccagcctgcg cttccaacgg cgtcacaccg
     1981 aacggatcac cgtcgatctg cacgggcacc ggatgcgacg ccgtaatttt cacatgcgca
     2041 cattgatgca tgaccacatc gccgttgcgc gcggcacgcg cgtcgagctg gccgcgcagc
     2101 aagctgagga gctggcccat caaaacaaca cggcttctcg ccctgaacag aacggcctcc
     2161 aggccgcgct gctggatgcc ggtccccggc gcgagcacaa accgcccggc gtagtggcgc
     2221 gcattggcaa tcaccaccca actcgcctcg tggcattgat tgtcgatcgt gacgctcaag
     2281 gtgtctggtg gccgcatgag ggcgccgagc acgggacccg tataggcgac cttgccgatg
     2341 cggctcttca gggcgtgatt gagggcgccg accacccgcg catcgaagcc ggcgccggcc
     2401 atcagcaaaa acggttcgcc attggccctg gggcaagcga tcgaaatcgg cgggccgtgg
     2461 cgcagcgtct ggcagacgag atcgggcttt gctttgagac cgatctcctg ggccagcacg
     2521 ttggcggtac cacagggaat gattgccaaa ggcacgtcag tgccattcat tcccgccgcc
     2581 gcggccctga tggtcccgtc tccgccggcg gccaccacga cgtcatagcg ccgagagcgt
     2641 gccgcggccg cggcctgtcg ccgaatcgcg gcgaattcgc gcagagtttc acgggtgaca
     2701 gaggcgcccg cgcgctccag cgcacgagcc acctcatcga ccagccaggg ccggctgagg
     2761 ccggccccgg cattgacgag cacaagaaat cgccggcgca tgctatgggg tgcggccaag
     2821 gttcatcgac cgattgtgcc gccaaagtgg aggcggcgca aatagccgat cggccggagg
     2881 cccgaggcaa atgtttaggc gccggcgggg ctggtggccc cttcttcgtc gcggcgggct
     2941 gatttctgca ccgccgcctg aaccttctcg aacgcccgca cctcgatctg acggatccgc
     3001 tcgcggctca ccccgaactc ggccgacaga tcttccaagg tcgccggttc ctccgccaag
     3061 cggcgcgcct cgaagatgcg cttctcgcgc tcgttcaagg tcgacattgc cttggcaagg
     3121 tagcctttgc ggagcgtcag ttcctccgat tcggcaaggc gctcttcctg gtcaggcgtg
     3181 tcatcgacaa gccagtcctg ccactcgccg ccgccttc
//
LOCUS       DASTMT010000500         2241 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_5068554, whole genome shotgun sequence.
ACCESSION   DASTMT010000500 DASTMT010000000
VERSION     DASTMT010000500.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2241
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5068554"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..942)
                     /locus_tag="VFR00_11470"
     CDS             complement(<1..942)
                     /locus_tag="VFR00_11470"
                     /inference="COORDINATES: protein motif:HMM:NF016999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HEU0159930.1"
                     /translation="MPLAGQYGLLLAGSLLLAVLLTVARLPAALLLGPMIAAIVLGSN
                     GARLSVPRRAVFACQTIVGCLIARSITGDIVHAFLRDWPLLLATVLAIIATSGVLGWL
                     ISRWQVLPGSTAVWGSAPGGASVMMIMAGAFGADARLVAFMQYLRVVLVAAVASLVAR
                     FWVGQAGPSAAVGWGSALHWGDFCALDWGGFFATLLIAAGGGAIGMALRIPAGALLVP
                     MVIAAVLEAMGLVFIVLPQWLLAITYAFLGWSIGLSFNREILQHAARALLPIMLAIGV
                     MISVCAALAALLVYYAGIDPLTAYLATSPGGMDSVAII"
     gene            complement(1065..1715)
                     /locus_tag="VFR00_11475"
     CDS             complement(1065..1715)
                     /locus_tag="VFR00_11475"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006314352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEU0159931.1"
                     /translation="MFYETRDNKHGLKHDPFKALCVPRPIGWISTVSKAGICNLAPYS
                     FFNAVGERPTYVMFSSAGRKDSLRNIEETGEFVCSLATYALRFNMNMTSAPVAHGVDE
                     FAIGDLTAAASRLVKPPRVKESPAAFECKHWKTIELPGIEPGKPSTYSVIFGQVVGIY
                     IDDSFIKDGLVDTGAMRPIARLGYMDYGVLNPENVFAIDRPTAEEGMAKLKVRAAE"
     gene            complement(1739..>2241)
                     /locus_tag="VFR00_11480"
     CDS             complement(1739..>2241)
                     /locus_tag="VFR00_11480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HEU0159932.1"
                     /translation="SAAELEAILTAAARVPDHKKLVPWRFIVFEGEARADFGRVLLKA
                     LQAEEREAPSAARLETERTRLMRAPTVVAVISRVTPNPAAPEWEQVLSCGAACFNLCL
                     AANALGFGTSWITEWYSYSRSVQAALGLARNERVAGFIYIGTAKEQQADRDRPQLEQI
                     VSRWNS"
BASE COUNT          415 a          732 c          754 g          340 t
ORIGIN      
        1 gatgatcgcc accgaatcca tgccaccagg gctggtggca agataggcgg tcaacggatc
       61 gatgccagcg tagtacacca atagggcggc aagcgcggcg cacactgaga tcataacgcc
      121 gatggcgagc atgatcggca gcagcgcgcg ggcggcgtgc tgcaggatct cgcggttgaa
      181 gctaaggccg atgctccaac ccagaaacgc gtaagtgatc gccaacagcc attgcggcag
      241 cacgatgaag acgaggccca tcgcctcgag caccgccgcg atcaccatcg gcacgagcag
      301 ggcgccggcc ggaattctca gcgccatgcc gatggcaccg ccgccggccg cgatcaaaag
      361 tgtggcgaag aagccgcccc agtcgagagc gcagaagtca ccccagtgaa gcgcgctgcc
      421 ccagccgacg gcggcgctcg ggccggcttg gccgacccaa aagcgggcga ccagcgaggc
      481 gaccgccgcc accaggacca cgcgcaaata ttgcatgaag gcaaccaggc gcgcatcggc
      541 accgaatgcg cctgccatga tcatcatgac cgaggcgccg cccggcgcgc tgccccacac
      601 tgcggtcgag ccgggcaaca cctgccagcg gctgatcagc cagccgagca cgccgctggt
      661 ggcaataatg gccagcacgg ttgcgagcag gagcggccaa tccctgagga aggcgtgcac
      721 gatgtcgccg gtaatcgagc gcgcgatcaa acagccaacg atggtctggc aggcgaacac
      781 ggcgcggcgc ggcacgctca ggcgggcgcc gttgctgccc agcacgatgg cggcgatcat
      841 cgggcccagc aacaaggcgg ccggcaggcg tgccacggtc aggagcacgg ccagcagcag
      901 cgaaccggca agaagcaggc cgtattgccc ggccaggggc atggctgcga agcaggcggt
      961 ccgccgcggc ttgggtgctg atggcatcgt gagccctggc gccggtgttc ccgctcgccc
     1021 gacggcgtgg ccgtcgagcg ggggcttggc ggggtggcgc gaggctattc cgccgcgcgc
     1081 accttgagtt tggccatgcc ctcctcggcc gtgggccggt cgatggcaaa gacgttttcc
     1141 gggttcagca cgccatagtc catgtagccg agacgcgcga tggggcgcat ggcgccggtg
     1201 tcgaccaggc catccttgat gaagctatcg tcaatgtaga tgcccacgac ttgaccgaag
     1261 atgaccgagt aggtcgacgg cttgcccggc tcgatgccgg gcagctcgat cgtcttccaa
     1321 tgcttgcatt cgaaggcggc ggggctctcc ttgacgcgcg gcggcttcac cagccggctt
     1381 gcggccgcgg tcaggtcgcc gatcgcaaac tcgtcgacgc catgcgccac cggtgccgag
     1441 gtcatgttca tgttgaagcg cagcgcgtag gtggcgagcg agcacacgaa ctcgcccgtc
     1501 tcctcgatgt tacgcagcga atccttgcgc ccggccgaac taaacatgac gtaggtcggc
     1561 cgttcgccga cggcgttgaa aaagctgtag ggggcaagat tgcaaatgcc ggccttgctg
     1621 acggtcgaga tccaaccgat cggtcgcggt acgcacagcg ccttgaaagg atcgtgcttc
     1681 agtccatgct tgttgtcgcg tgtctcgtaa aacacctgtt tgcctctcct gcgttggctc
     1741 agctgttcca gcggctgacg atctgctcga gctgcgggcg gtcgcgatct gcttgttgct
     1801 ccttggcggt gccgatgtag atgaagccag cgacacgttc attgcgcgcg agcccgagcg
     1861 ctgcctgcac gctgcggcta taggagtacc attccgtgat ccaggaggtg ccgaagccaa
     1921 gcgcgttggc agccaggcaa aggttgaagc aggcggcacc gcaggacaac acctgctccc
     1981 attccggcgc cgccggattg ggcgtcaccc gcgagatgac cgccaccacc gtgggcgccc
     2041 gcatcagccg cgttcgctcc gtctccaagc gcgccgccga cggcgcctcc cgctcctcgg
     2101 cctgcaaggc ctttagtaag acgcggccga agtcggcgcg ggcctctccc tcaaagacga
     2161 tgaaacgcca gggtacgagc ttcttgtggt cggggacgcg ggccgccgcg gtcaggatgg
     2221 cctcaagctc ggcggcgctt g
//
LOCUS       DASTMT010000501         3456 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_4218564, whole genome shotgun sequence.
ACCESSION   DASTMT010000501 DASTMT010000000
VERSION     DASTMT010000501.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3456)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3456)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3456
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4218564"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..196
                     /locus_tag="VFR00_11485"
     CDS             <1..196
                     /locus_tag="VFR00_11485"
                     /inference="COORDINATES: protein motif:HMM:NF025589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4332 domain-containing protein"
                     /protein_id="HEU0159933.1"
                     /translation="AQLLVGAGYSTAEAIAAADPERLCADVINFALTATGQRLLRQAE
                     MPDLQKVKAWHEAARSIKAA"
     gene            complement(262..678)
                     /locus_tag="VFR00_11490"
     CDS             complement(262..678)
                     /locus_tag="VFR00_11490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011005215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system Fe-S cluster assembly protein"
                     /protein_id="HEU0159934.1"
                     /translation="MDEARTNAGPADATAADVAQPPTSVTAAPGSAIPTEELEQLTAD
                     IVGALKTVYDPEIPADIYELGLIYKIDIDDQRNVAVEMTLTAPGCPVAGEMPVWVENA
                     VSGVAGVGQVSVQLTFDPPWEQSRMSDEARLALGMF"
     gene            complement(708..1967)
                     /locus_tag="VFR00_11495"
     CDS             complement(708..1967)
                     /locus_tag="VFR00_11495"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEU0159935.1"
                     /translation="MTAMIKGAGLKAGSNYDVETIRTDFPILFREVYGKPLVYLDNGA
                     SAQKPRPVLEAIEHAYKMEYANVHRGLHYLSNTATQRFEDARETVRRFLNAASPEEII
                     FTRNATEAINLVAASYGGMSIGEGDEIVISIMEHHSNVVPWHFHRERRGAVLKWAPIS
                     DSGEFLLDRFESLLSARTKMVAITHMSNVLGTVVPIKEVVRIAHARGIPVLVDGSQAA
                     VHMTVDVRDLDCDFYAFTGHKTYGPSGIGVLYAKRRHLDAMPPYMGGGEMIESVSEDA
                     ISYGRPPHKFEAGTPPIVQAIGLGAALNYMMQVGRDRIARHEAELKDYAHERLAALNW
                     LKLYGTAADKGAIVAFGIDGLHPHDISTIIDRSGVAVRAGHHCAQPLMHRLGVTATCR
                     ASFAMYNTKAEVDALAEALIKAHDFFA"
     gene            complement(2001..3329)
                     /gene="sufD"
                     /locus_tag="VFR00_11500"
     CDS             complement(2001..3329)
                     /gene="sufD"
                     /locus_tag="VFR00_11500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HEU0159936.1"
                     /translation="MTVAITRTRAEQGLSQTFETVAPQLPGTPAVAAARRAAIGAFAA
                     LGLPHRRLEAWKYTDLRSALKEALPAAVGEERALPSRAELETALEALAELEAHRLVFV
                     DGLYAPGLSTATALAGLTFTPLAARLRHAGAEPNQQLLASGEREAVIALNTAFMSDGA
                     VLEVAPGLTLAKPVLLVFLRTAGKARLTTTRNQISIGAGARASLIEAHLGLPAAPGGQ
                     SNTLSELSLGKDAELSHIKVTLGGPGESHVSTGLPRLGAGASYRAFQLTAATSLVRNG
                     VGLTFDGEGGKADVSGCFLGRGSEHIDSTLVIDHAVAGCESRELFKGVLADKARGVFQ
                     GKVIVRPNAQKTDGKQMAQVLMLSEDAEFDSKPELEINADDVVCGHGSTSADIDEDLL
                     FYFRARGIAPEEARALLIESFIGEAIDKVEETLRPALMTMARARLAELTS"
BASE COUNT          568 a         1145 c         1129 g          614 t
ORIGIN      
        1 cgcgcagctg ttggtcggcg ccggctacag cactgccgag gccattgcgg cggccgaccc
       61 cgaacggctc tgcgccgacg tcatcaattt tgccctgacg gcgacgggcc aacgcctgtt
      121 gcggcaggcc gagatgcccg acctgcagaa ggtcaaggcc tggcacgaag ctgcgcgcag
      181 catcaaagcg gcttagccaa ggggagcgcc gccggcagcc gtgcggccgc accgctgggc
      241 gcggcgaggg ggagccccgg cctagaacat gccgagcgcc aaacgcgcct cgtccgacat
      301 gcgcgactgc tcccagggcg gatcgaaggt cagctgcacc gagacctggc cgacgccggc
      361 caccccgctc acggcattct ccacccaaac cggcatttcg ccggcgaccg gacagccagg
      421 cgccgtgagc gtcatttcca cggcgacgtt gcgctgatcg tcgatatcga tcttgtagat
      481 gaggcccagc tcgtagatat cggcgggaat ctcggggtca tagacggttt tgagcgcgcc
      541 gacgatatcg gccgtgagct gttcgagctc ctcggttgga attgccgacc ccggcgcggc
      601 cgtcacggac gtgggcggct gcgcgacatc ggccgctgtc gcatccgcgg ggcctgcgtt
      661 tgtcctggcc tcatccatgg cctcttcctt tgcttggcac ttcgccgtca ggcgaagaaa
      721 tcgtgcgcct tgattagagc ctcggccagc gcgtccacct cggccttggt gttgtacatg
      781 gcgaaggaag cgcgacaggt ggcggtcacg ccaagccggt gcatcagcgg ctgggcgcag
      841 tgatgaccgg cacgcaccgc cacgcccgag cgatcgatga tggtcgaaat atcatgcgga
      901 tgcaggccat cgatgccaaa ggcgacgatg gcacccttat ccgccgcggt gccgtagagt
      961 ttcagccagt tcagcgcggc caagcgctcg tgcgcatagt ccttgagctc ggcctcgtgg
     1021 cgggcgatgc gatcgcgacc aacttgcatc atgtagttga gcgcggctcc gagcccgatc
     1081 gcctgcacaa tggggggcgt gccggcctcg aacttgtgcg gcggccgccc atagctgatg
     1141 gcgtcctcgc tgacgctttc gatcatttca ccgccgccca tatagggcgg catggcatcg
     1201 agatggcgcc gcttggcgta gagcacgccg atacccgagg ggccgtaggt cttgtgcccg
     1261 gtgaaggcgt agaaatcgca atcgagatcg cgcacgtcga ccgtcatgtg cacggccgcc
     1321 tggctgccat cgaccagcac cgggatgccg cgcgcatgcg cgatcctgac gacctccttg
     1381 atcgggacga cggtgcccag cacgttcgac atgtgggtaa tggcaaccat tttggtgcgc
     1441 gcggagagca ggctctcgaa gcgatcgagc agaaactcgc cgctgtcgct gatcggcgcc
     1501 cacttgagga cggcgccgcg ccgctcgcga tgaaaatgcc agggcacgac gttggagtgg
     1561 tgctccatga tggaaatgac gatttcgtcg ccttccccga tgctcatgcc gccataggag
     1621 gccgccacca ggttgatggc ctcggtggca ttgcgcgtga agatgatctc ctccgggctg
     1681 gcggcattga ggaagcgacg gacggtctcg cgcgcgtcct cgaaccgctg cgttgcggta
     1741 ttggagagat agtgcaagcc acgatgcacg ttggcgtatt ccatcttgta ggcgtgctcg
     1801 atggcttcca gcaccggacg cggcttctgc gcggaggcgc cattgtcgag gtagaccagc
     1861 ggcttgccgt agacctcacg gaacagaatg ggaaagtccg tgcggatggt ctcgacgtcg
     1921 taattgctgc ccgccttgag cccggcgccc ttgatcatcg cggtcataac caatcctttg
     1981 cccttgccaa ccgcgccatc tcaactcgtc aactcggcca agcgggcccg cgccatcgtc
     2041 atcaaggcgg gacgcagcgt ctcttccacc ttgtcgatcg cctcgccgat gaagctttcg
     2101 atcagcagcg cgcgcgcttc ctccggcgca atgccgcggg cacgaaaata gaagagtagg
     2161 tcttcgtcga tgtccgccga ggtcgagccg tgaccgcaga ccacgtcgtc ggcgttgatc
     2221 tcgagctcgg gcttggaatc gaactcggcg tcctcggaca gcatcagcac ctgcgccatc
     2281 tgcttgccgt cggtcttctg cgcgttcggg cgcacgatca ccttgccctg gaagacgccg
     2341 cgggccttgt cggccaacac gcctttgaag agttcgcggc tttcgcagcc ggcgaccgcg
     2401 tggtcgatca cgagcgtgct atcgatgtgt tcgctgccgc gcccaaggaa acaaccggag
     2461 acatcggcct tgccgccttc gccgtcgaac gtcaggccga cgccattgcg caccagggag
     2521 gtggccgcgg tcaactggaa ggcgcggtag ctggcgccgg cgccgagcct cggcagcccc
     2581 gtggaaacgt gactttcccc cggcccgccg agcgtcacct tgatgtggga gagctcggca
     2641 tccttgccga ggctcagctc gctcaaggta ttgctctgtc cgcccggggc cgcaggcagt
     2701 cccagatgcg cctcgatcag gctggcgcgc gcgccggcgc cgatgctgat ctgattgcgc
     2761 gtggtagtga ggcgcgcctt gccggccgtg cgcagaaaga cgagcaatac cggcttggcg
     2821 agcgtcaacc caggcgcgac ctcgagcacg gcaccatcgc tcatgaaggc cgtgttgagg
     2881 gcgatcacgg cctcgcgctc gccagacgcc agcagctgtt ggttgggctc ggcgccagca
     2941 tggcgcaagc gcgccgccag cggcgtgaag gttaagccgg cgagcgccgt tgccgtcgac
     3001 aatccggggg cataaaggcc atcgacgaac accaggcggt gcgcctcgag ctccgccagg
     3061 gcctcgagcg cggtctcgag ctcggctctg cttggcaaag cgcgctcctc gccgaccgcg
     3121 gccggcaagg cctccttcag cgccgagcgc aggtcggtgt acttccaggc ctcgagccgg
     3181 cgatgcggca agccgagcgc cgcaaaggcg ccaatggcgg cccgccgggc cgccgccacc
     3241 gccggggtac ccggcaactg cggcgccacc gtctcgaagg tctggctgag cccctgctcg
     3301 gccctcgttc tcgtaatcgc cactgtcatc tcacgttcac tttttctggc actttttttg
     3361 gcagttcttt gccagtgttc tttggcactt attgcaccgt ggcagcgcgt tacgctgccc
     3421 ggttgcggaa ctcggcgtag ccggtcttct ccaact
//
LOCUS       DASTMT010000502         2641 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_617968, whole genome shotgun sequence.
ACCESSION   DASTMT010000502 DASTMT010000000
VERSION     DASTMT010000502.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2641
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_617968"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1175)
                     /locus_tag="VFR00_11505"
     CDS             complement(<1..1175)
                     /locus_tag="VFR00_11505"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEU0159937.1"
                     /translation="MLNEAPLSQFQPLGGGLVRPIYADYSFGNITNTVEFLLTGAARR
                     PLLPADCFGGAYPRPEKVVVFFVDSFAWQFWCQYRRRFRTLSRVSEEGTLTPLSALFP
                     STTAASVSTMNLGVLPAQHALYEWNIYIPAYGEVIQSLAFTPLGRHPPDACLGLGYDP
                     AKLLEVHETVHQRLKERGVRSIQFAHRSYAGSAYNRLACAGAEIVRHTTLAEALVQLK
                     ECLLGTPGKALFSFYWAAIDSIAHTHGPGSSYHAAEIASFWHTVDAIFSGVPASDTLY
                     LFTADHGHVRADARDTLYLNEALPAVADCLPRSPTGKPIYPNGSPRDVFLHVRPQRRA
                     DVLGLLQRELATQALVLPMQEAAALGLFGSGTISEELRRRLGDILILPYSGRFIWWR"
     gene            complement(1298..2146)
                     /locus_tag="VFR00_11510"
     CDS             complement(1298..2146)
                     /locus_tag="VFR00_11510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015933394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEU0159938.1"
                     /translation="MQFQGTKTYIATDDLKVAVNAAITLQRPLLIKGEPGTGKTVLAI
                     EVAKAIKAPLIEWHIKSTTKALQGLYEYDAVSRLRDSQLGDPRVADIRNYIRKGKLWE
                     AFTMGERPVLLIDEIDKADIEFPNDLLQELDRMEFYVYETHETIQAKHRPIVIITSNN
                     EKELPDAFLRRCFFHYIKFPDPDTMRAIVDVHFPDIKGRLVSEALKVFYDVRDVPGIK
                     KKPSTSELLDWIKLLLNEDISPETLRENDPRKLIPPLHGALLKNEQDVHLFERLAFIA
                     RRQNQG"
BASE COUNT          486 a          793 c          905 g          457 t
ORIGIN      
        1 tcgcgccacc agatgaaccg ccccgagtag ggcaaaatca aaatgtcgcc gagccgccgg
       61 cgcaattcct cgctgatcgt gccgctgccg aatagtccca gcgccgccgc ctcctgcatc
      121 ggcagcacca gcgcctgcgt ggcaagttcg cgctgcagca gtcccagcac gtccgctcgg
      181 cgctgcggcc gcacgtgcag gaaaacgtcg cgtggcgagc cgttgggata gatcggcttg
      241 ccggtgggac tgcgcggcag gcagtcggcg acggccggta atgcctcgtt gaggtaaagg
      301 gtgtcgcgcg cgtcggcgcg cacgtgtccg tggtcggcgg tgaagagata aagcgtgtcg
      361 gacgcgggca cgccactaaa gatcgcatcg acggtgtgcc agaagctcgc aatctcggcg
      421 gcatgatagc tgctgccggg tccgtgcgtg tgggcaatgc tatcgatggc ggcccaatag
      481 aagctgaaga gtgccttgcc tggcgtgccc agcaggcact ccttgagctg gaccagcgct
      541 tcggccagcg tcgtgtggcg cacgatttcg gccccggcac aggcgagcct attgtaggcc
      601 gacccagcgt aactgcgatg cgcgaactgg atggagcgca caccccgttc cttgaggcgc
      661 tgatgcacgg tttcgtggac ctcgagcaac ttggccgggt cgtaacccag acccaggcag
      721 gcatccgggg gatggcggcc gagtggcgta aaggccagcg actggatcac ctcgccatag
      781 gccgggatgt agatgttcca ctcgtagagc gcatgctgcg ccggcagcac gccgaggttc
      841 atagtcgaga ccgaggccgc ggtcgtggag gggaatagcg ccgaaagagg ggtcagcgtg
      901 ccctcctcgc tgacgcgaga cagggtacga aaccgccggc ggtattggca ccaaaactgc
      961 caggcaaagg aatcgacgaa gaagacgacg accttctcgg gtcgcggata ggcgcccccg
     1021 aagcaatcgg cgggcagcag gggccggcgc gccgcgcccg tcagcaggaa ctcgacggtg
     1081 ttggtgatat tgccgaagct gtagtcggca taaatcggcc gcacgaggcc gccgcccagg
     1141 ggctgaaact gcgaaagcgg cgcttcgttg agcatggggc gggcgcctgg ctcaggctcg
     1201 atcgtgggca gcggcgagaa gctttggcac catgcgccga cggcggcttg ggcgcaattg
     1261 ctgccgtcgg atgccctcca tcgtgcccag aatcgggcta accctggttc tgccggcgcg
     1321 cgatgaacgc taggcgctcg aacagatgca cgtcttgctc gttcttgagc agcgccccat
     1381 gcaggggcgg gatcagcttg cgcgggtcgt tctcgcgcag cgtctcgggg ctaatgtcct
     1441 cgttgaggag cagtttgatc caatcgagca attcggaggt ggaaggcttc ttcttgatac
     1501 ccggcacgtc ccgcacgtca tagaagacct tcaatgcttc cgacaccagg cgccccttga
     1561 tatcgggaaa atgcacgtcg acgatggcgc gcatggtgtc ggggtcggga aacttgatgt
     1621 agtgaaaaaa acagcggcgc aggaaagcgt ccggcagctc tttctcgttg ttggacgtga
     1681 tgatgacaat cggccggtgc ttggcctgga tggtctcgtg cgtttcgtaa acataaaact
     1741 ccatgcgatc gagttcctgc aggagatcgt tggggaactc gatgtcggcc ttatcgatct
     1801 catcgatcaa cagcacgggc cgttcaccca tggtgaaggc ctcccacaac ttgcccttgc
     1861 ggatgtagtt gcggatgtcg gcgacgcgag ggtcgccaag ctggctatcg cgcaggcggc
     1921 tgaccgcgtc gtactcgtaa agaccctgca aagccttggt ggtcgacttg atgtgccatt
     1981 cgatcagcgg cgccttgatc gccttggcga cctcgatggc gagaaccgtc ttgccggtgc
     2041 cgggctcgcc tttgatcagc aggggtcgct gcaaggtgat ggcggcattg acggcgacct
     2101 tgagatcgtc agtggcaatg taggttttgg tgccctggaa ctgcattggc tggcggagcg
     2161 ctccccggct ggcaaaagaa cgcgcgagct tagggggtga ggggtggtaa gccaagcgcg
     2221 cctgctgcgg caaaggcgtg cgctggcgca tcgccgcgcc ttgcgcgcgt caggcggctc
     2281 cgcgcgcaca aagggtgcga cctcgcgaag gcgtggcgcg ccgaggggcg ggcgctgccg
     2341 gtcggcaaaa acctcccgcg cgcttgacca agacccgccc ggcagcccag cgcccgaaga
     2401 gcgccatgcc cctcccgccc accccgtgcc ctgtgcgcgt gccgcggggc ggctgcacga
     2461 agtcggccca agccaaagcc ggcgccaggt cttcgggagg agaggaggca tatgcaacca
     2521 tgtgtggccg gatgcggctg cagcagccca atccgccgcc ggtacgggct caaggggcca
     2581 cttctacacg gaaagcgtgt atttccttat aaatcaaatc tggggttgtc caagctgccg
     2641 g
//
LOCUS       DASTMT010000503        12144 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_3750558, whole genome shotgun sequence.
ACCESSION   DASTMT010000503 DASTMT010000000
VERSION     DASTMT010000503.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 12144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12144
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3750558"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..612
                     /locus_tag="VFR00_11515"
     CDS             <1..612
                     /locus_tag="VFR00_11515"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009008 - DNA-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HEU0159939.1"
                     /translation="DWLFPICSGPERLKDAAGRKAHPTQKPEALLHRILLAASNPGDL
                     VLDPFFGTGTTGAVARRLGRRWLGIERSAAYAAAARERIARVRPYSPSALETAPAKRL
                     EPRVAFGTIIELGILEPGVTLTDERGGLAAEVKADGTLAVKGRQGSIHRLGAELQGKA
                     ACNGWTFWHYEVEGKRKPIDALREEARRQLGLSAGPIALVAAE"
     gene            complement(577..939)
                     /locus_tag="VFR00_11520"
     CDS             complement(577..939)
                     /locus_tag="VFR00_11520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0159940.1"
                     /translation="MSKRAYLFEVFRREAIPDLGEIGAVYVYARTAPPDAASSLPRDA
                     GGTAYEPGYIGSTAELARREAEHDRHGHFIGFAFDVVLVLRLEQETIRTDIEADLIGL
                     YRPVLNELLRGYQRDGSS"
     gene            1111..2526
                     /locus_tag="VFR00_11525"
     CDS             1111..2526
                     /locus_tag="VFR00_11525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018388959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0159941.1"
                     /translation="MSWKRPWAAGAHVAETVPRGDLDRRARLAAALTVYLRPRVLVVL
                     LLGFSAGLPLALSGETLRVWLADCGVDLATIGLLTLAGLPYTIKFLWAPVVDALDIPW
                     LSKRFGRRRGWLIATQLVLMGTVVFLGTRAPQGAPLLVGLAALIVAFASATQDVLIDA
                     YRVESLAPEEQAAGMASYVAAYRVGGLVSTAGVIALSAWFEAEGLARTSAWSLAYLAA
                     AGLVLIGVAATLLGADVARPQVPPRPGQGTLKRIAETASGAFSDFLSRADALAILALI
                     ILFKLCDALAGTMTGPFVLGLGYDKATYAAVVKGVGLAALLGGGFAGGAIARTTPMSL
                     ALWLGASLQMLSNLAFVWLNFQTPGTGALTVVIVIENFTGAMGTVFFVAYLSALCRNP
                     LHTATQYALLTALASTARTLLSASTGALAALIGWPLFFLTTTLAALPALAVLALLQRR
                     GHFAALEAGRETAAADGLSAP"
     gene            complement(2513..3619)
                     /gene="mutY"
                     /locus_tag="VFR00_11530"
     CDS             complement(2513..3619)
                     /gene="mutY"
                     /locus_tag="VFR00_11530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019400899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEU0159942.1"
                     /translation="MANVARAQPTACPAGSDTADLLLAWWDRERRELPWRTAPGRAPD
                     AYRIWLSEIMLQQTTVKAVIPYYERFLARWPTVEALAAAQQDEVLAAWAGLGYYSRAR
                     NLYKCAKLIAEAYAGKFPSTSAELVHLPGIGPYTAAAIAAIAFGEPATPVDGNIERVV
                     ARLFAVRDPLPAAKAQLHHLAGSLTPPARAGDFAQALMDLGAGLCTPKRPSCLVCPLQ
                     TKCAGNAEGLAAQLPFKPSKADRPLRVGLAFVALREDGSVLLRRRPDAGLLGGMLEVP
                     STEWSDALPPLDQALRAAPVHGQWWSVPGTVAHTFTHFRLELLVYRAVVPNEAALTFW
                     ADPQRCRWVPRRNLNGEALPSVMKKIVAHGLKEH"
     gene            3704..4282
                     /locus_tag="VFR00_11535"
     CDS             3704..4282
                     /locus_tag="VFR00_11535"
                     /inference="COORDINATES: protein motif:HMM:NF017103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DciA family protein"
                     /protein_id="HEU0159943.1"
                     /translation="MTLSPVRGSPAHPLPRQALAFARPTARSLKVKAVGSFLPSLTAQ
                     AFKKFGFSTAQLIMDWPAIAGKDLAAHSLPERLKWPPRPEAEAEEGEPAQGRRGATLL
                     LRVNGARALEIDYQRQQILERVNAYFGYRAVSELRLVQAPLPAAPTAPVRPPRAAACP
                     PPCTEVVGIPDAALREALARLGSRLGARLAPR"
     gene            4497..5195
                     /locus_tag="VFR00_11540"
     CDS             4497..5195
                     /locus_tag="VFR00_11540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HEU0159944.1"
                     /translation="MNAFEPINVHRALSRRATFKFALGLAVGAAGVSWGLAAAAQKRA
                     QGRAEIPIDELMQPGPLPELVLGKEDAPITVVEYASMTCGHCANFHNKVFPTLKEKYI
                     DSGKVRFVMREFPLDKLAVAASMLARCAGGEKTFPLISALFAKQEEWAFVRGNPVPEL
                     FKLAKQAGFTQESFDKCLTDQALMDDIVKIRGRAADVFGVGSTPTFFINGKKMTGAPT
                     VEEFDKAFAALLKS"
     gene            5318..6037
                     /locus_tag="VFR00_11545"
     CDS             5318..6037
                     /locus_tag="VFR00_11545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HEU0159945.1"
                     /translation="MPLLGEPPPTGAALEAGGATVDRPGFDPFQATVPGPAPSRQILQ
                     NPTLGDILKTGELPEMALGRKDAPVTIVQYASMTCPYCRRFQAETFPVLKREYIDTGK
                     VRYVLRAEFPIGKQSGLATIALRCAPPEQYFVLYDKLMSQQATWVSQEVRPDPIFKVA
                     AQVGMTRAQFDSCRENRAMIGQLNWIKERGRTLGIIGTPNFFIQGKLVKSVLGMKEIR
                     EMVDPMLAGQVAVSGPREGAR"
     gene            6136..9594
                     /locus_tag="VFR00_11550"
     CDS             6136..9594
                     /locus_tag="VFR00_11550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013949759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003682 - chromatin binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007076 - mitotic chromosome condensation
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation SMC family protein"
                     /protein_id="HEU0159946.1"
                     /translation="MHINRLRLLGFKSFVEPTELLVEPGLTGVVGPNGCGKSNLLEAL
                     RWVMGESSHKSMRAAAMDDVIFSGTADRPARNTAEVTIFLDSAGNKLPAEFHAGETLE
                     VTRRIEREAGSAYRINGREARARDVRILFEDAATGARSPALVRQGQIGELVNAKPEQR
                     RRILEDAAGIAGLHTRRHEAELRLKAADANLARIQDILGQLYSQIESLKRQARQARRY
                     KDLSSEIRKGDALVLHLSWQEAQTQVSTGETALAGALEKLALATELESKALRAEADAA
                     GRLPPLRDQEAAKSAVLHRFKVEQDNLEREARRTEERCQELEARSEQLRRDLEREDLL
                     RREAQETLARLEAERQSLANTAALAADFEQKALESFEAGDARLAEAERRLQHLTTAAA
                     EARARRQSLEAQRQERQGQIEKLTSQLVALEAQTREIVGRAPDASKLKVTAEAGQRLM
                     TEIAVIEAQALTAEEAAQAAALEAEQKSETARAADLAAGKLKTEVETLKKLLKPSDAG
                     GLPPILDEIRVAAGYEMALAAALGDDLEAPAVEAAALHWRLNPGDERDPALPAGAEPL
                     SAYVTAPPQLARRLAQIGLVEQSEGGRLLRQLRSGQRLVSRQGDLWRWDGYVAAAQGT
                     SAAASRLAERSRLGMLEAEEASQRQIAEVARAASAAADQRHKAAQTEERRLRQLWRET
                     QGQLAQTREVLTGLERQARETEAKLAAVTAAKQKSTEALAEVQDLLAKSENALGALAG
                     IEALEAELAAAQTDTNGLRGQVAETKTELVTLEREHRARVERQKAIGLENERNLARIA
                     SAEEQIVALKDRAAGTDAELARLADIPRMVEQQRQTLMTELAAAEHDRRNAADTLAAA
                     ETTHRQAAQDLRAAQTAVADERERRARSEAHLENARQRRAEEGRKIRDQLGCAPEDCL
                     ALAGLAGEAALPSLEAALGGLNRLKAERERLGGVNLQADDDLAGLAQQFESLDKEKRD
                     VEAAIAKLRAGIQQINNEGRTRLQAAFDAVNGHFKQLFMTLFGGGEARLEMIDAEDPL
                     DGGLEIIAKPPGKKPATLSLLSGGEQSLTAMALIFAVFLTNPSPICVLDEVDAPLDDA
                     NVDRFCTLLEKMAADTATRFLVITHHPMTMSRMNRLFGVTMAEKGVSQLVSVDLETAQ
                     KFVVAA"
     gene            9621..10283
                     /locus_tag="VFR00_11555"
     CDS             9621..10283
                     /locus_tag="VFR00_11555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016848208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HEU0159947.1"
                     /translation="MHVLLDLDGTLLDPGAGLIASIQYALKASGKPVPPAAELGWMIG
                     PPLRASFAELLGGTEPAEAALAHYRAYFGARGMYEATVYPGIPEALDALAGAGCRLIV
                     ATAKPHHFARPILKHFALADRFAAIHGPELDGTRDAKADLIAFILDREGVAVDAALMV
                     GDRAVDVRAARSNGMRSVAAAWGYGTPTELSAAGAAAICETPQALAPLVLALLGAERR
                     PR"
     gene            10477..10887
                     /locus_tag="VFR00_11560"
     CDS             10477..10887
                     /locus_tag="VFR00_11560"
                     /inference="COORDINATES: protein motif:HMM:NF021073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HEU0159948.1"
                     /translation="MPDPTDHRGPGERQMSPEEAAAFQGRVAELGDRLGKIKAQRDAD
                     AHADLDAEMRGRGMAYGMRMAAEMVGAVIVGGGIGWGLDWWLGSKPWLFLLFFLLGFA
                     AGVLNVVRGYERMQKDFMARTGGRLGHSVPDDED"
     gene            10934..11677
                     /locus_tag="VFR00_11565"
     CDS             10934..11677
                     /locus_tag="VFR00_11565"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008549614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEU0159949.1"
                     /translation="MPDPIHQFEIHRIIPIKIFGWDASFTNASLFMLLAVLLIAGFFV
                     VSMQTRSLVPSRLQSLAEVSYEFVAGMLRESVGHAGMKYFPFVFTIFIFVFTANTLGL
                     IPGFFTVTSHIVVTAALAAMVFLTVLVVGFAKNGVKFLQLFVPQGVPILILPLVVLIE
                     IISFLSRPVSHSVRLFANMLAGHITLKVFGGFVVMLLGAGTYAALAPLPLVMAIALTA
                     LEVLVAFLQAYVFTMLTCMYLNDALHPGH"
     gene            11731..11955
                     /locus_tag="VFR00_11570"
     CDS             11731..11955
                     /locus_tag="VFR00_11570"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008595464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HEU0159950.1"
                     /translation="MDPAAAKLIGAGLAAIGTGAAAIGVGNLFGQFLQGALRNPSAAD
                     GQFGRLIFGFAVTEALGIFALLIAFLLLFT"
     gene            11984..>12144
                     /locus_tag="VFR00_11575"
     CDS             11984..>12144
                     /locus_tag="VFR00_11575"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012383748.1"
                     /note="Produces ATP from ADP in the presence of a proton
                     gradient across the membrane. Subunit B' is part of the
                     membrane proton channel; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP F0F1 synthase subunit B"
                     /protein_id="HEU0159951.1"
                     /translation="MATNSTAGTTTAHATGQPKVFPPLDSGTFAPQLIWLALSFGLLY
                     LLLKRFYLPR"
BASE COUNT         1959 a         4016 c         4137 g         2032 t
ORIGIN      
        1 gactggctgt tccccatctg ctcggggccc gaacggctga aggacgcggc gggccgtaag
       61 gcgcacccga cgcaaaagcc cgaagcgctc ctacaccgca tcctgttggc cgcgagcaat
      121 cccggtgatc tggtgctcga ccccttcttc ggaacgggca cgacgggcgc ggtcgcccgt
      181 cgcttgggac ggcgctggct cggcatcgag cgcagcgcag cctatgccgc ggcggcgcgc
      241 gagcgcatcg cccgggtgcg tccctattcg ccctcggcct tggaaacggc gccggccaag
      301 cgcctggagc cgcgggttgc cttcggcacc atcatcgagc tgggtatcct ggagccgggg
      361 gtgaccttga cggacgagcg tggcgggctc gccgccgagg tcaaggccga cggcacactg
      421 gccgtgaaag gccggcaggg ctccattcac cggctggggg cggagctgca aggcaaggcc
      481 gcctgcaacg gctggacctt ctggcactat gaggtcgaag ggaaaagaaa accgatcgat
      541 gcgctgcgcg aggaggcgcg gcgccagctc gggttgtcag ctggacccat cgcgctggta
      601 gccgcggagt agttcgttga gcaccgggcg ataaagcccg atcaggtcag cctcaatatc
      661 ggtgcggatc gtctcctgct ccaatcgcag cacgaggacg acatcaaagg caaagccaat
      721 aaaatggccg tgccgatcgt gctcggcctc gcgccgcgcg agctcggccg tgctgccgat
      781 gtagcccggc tcgtaggccg tgccgccggc gtcgcgtggc aaactggagg cagcgtctgg
      841 cggcgccgtg cgagcataga cgtaaacggc gccgatctcc cccagatcgg gtatcgcctc
      901 gcggcggaaa acctcgaaca ggtaggcgcg cttggacatg cctctgagcg tggcttggcc
      961 aggctttttt ttgccgcccc cgaacagacc gcgtgcagat cccggcttga gctggccaag
     1021 aagcccgcct accagagagg ccatcgttga tccccgcttg tcgtgccatc cgtgtggcgc
     1081 gggaatagga tgattcgggt tggacttaca atgagttgga aaaggccgtg ggcggcggga
     1141 gcgcacgtcg cggagaccgt gccaaggggg gatcttgatc gccgcgcccg gctggctgcg
     1201 gccctcacag tctatctgcg cccgcgcgtg ctggttgtgc tgttgcttgg cttttctgca
     1261 ggccttcccc tagcgctttc cggtgagacg ctgcgcgtgt ggttggccga ttgcggcgtc
     1321 gaccttgcca ccattggtct gctgacgctt gccggcctgc cctacacaat caaatttttg
     1381 tgggcccccg ttgtcgatgc gctcgacatc ccctggttgt caaagcgctt cggccggcgg
     1441 cggggctggc ttattgccac gcaactcgtg ctgatgggga ccgtcgtttt cctcggtacg
     1501 cgcgcgccgc agggcgcgcc gttgctcgtc ggcctggccg ccctgatcgt cgcctttgcc
     1561 tccgccacgc aagacgttct tatcgatgcc tatcgcgtcg agagcctggc gcccgaggag
     1621 caggcggcgg gcatggcgag ctatgtcgcg gcctatcggg tgggcggcct cgtctcgacg
     1681 gcgggcgtga ttgcgctctc cgcctggttc gaggcggagg ggctggcgcg cacctccgcc
     1741 tggtccttgg cctatctcgc cgcggccggc ctggtgctga tcggcgtggc cgcgacgctg
     1801 ctgggcgccg acgtcgcgcg cccccaggtc ccgcccagac caggccaggg caccctcaag
     1861 cgcatcgcgg agaccgccag cggcgccttc agcgacttcc tctcgcgcgc ggatgccctt
     1921 gccatcctgg ccctcatcat cctcttcaaa ttgtgcgacg cgctcgctgg caccatgacc
     1981 ggtcccttcg tgctgggcct cggctatgac aaggccacct acgcggccgt cgtgaagggc
     2041 gtcggcctcg ccgcgttgtt gggcggcggt tttgcggggg gcgccatcgc ccgcacgacg
     2101 ccgatgagcc tggcgctctg gctcggcgcc agcctgcaaa tgctctccaa tctcgccttc
     2161 gtgtggctca actttcagac gccgggtacc ggggcgctga ccgtcgtcat cgtcattgag
     2221 aattttaccg gcgccatggg tacggttttc ttcgttgcct atctctccgc gctttgccgc
     2281 aaccccctgc atacggcgac gcaatatgcg ctcttgaccg cgctcgcctc caccgcgcgc
     2341 acgctcctgt cggccagtac cggcgcgctc gcggcgctga tcggctggcc gctgtttttc
     2401 ctgacgacga cgctggcggc gctgcccgcc ctggcggtgt tggccctgct gcagcggcgc
     2461 gggcacttcg ccgcgctcga ggcggggcgc gagaccgccg cggccgacgg cctcagtgct
     2521 ccttgaggcc gtgcgcgacg atcttcttca ttacgctcgg caaggcttcc ccgttgaggt
     2581 tgcggcgcgg cacccagcgg cagcgctgcg gatcggccca aaacgtgagc gccgcctcgt
     2641 tgggcacgac ggcgcggtag acgagcagct cgaggcggaa atgcgtaaag gtatgcgcca
     2701 cagtgccggg caccgaccac cattggccgt gcaccggggc ggcgcgcagg gcttggtcga
     2761 gcggcggcag cgcatcgctc cactccgtcg agggcacctc gagcatgcct ccgagcagac
     2821 ccgcatcggg gcgacggcgc aataatacgc tgccgtcctc gcggagcgcc acgaacgcga
     2881 gcccgacccg caacggccga tcggccttgc tgggcttgaa ggggagttgg gcggcgaggc
     2941 cttcggcatt gccggcgcac ttggtttgca aggggcacac caagcaggaa ggccgtttcg
     3001 gcgtgcagag gcccgcgcca aggtccatca aggcctgggc gaagtcgccg gcccgtgccg
     3061 gcggggtcag gctgccggcg aggtggtgca gctgggcttt ggccgccggc aatgggtcgc
     3121 gcacggcgaa caggcgcgcc acgacccgtt cgatattgcc atcaacgggc gtggcaggct
     3181 cgccgaaggc gatcgcggcg atcgccgcgg cggtataagg gccaataccg ggcaagtgca
     3241 cgagttcggc ggaggtcgac gggaactttc cagcgtacgc ctcggcgatc agcttggcgc
     3301 acttataaag gttgcgcgca cgactgtagt agccaaggcc tgcccaggcc gccagcacct
     3361 cgtcttgctg cgcggcggcc agcgcctcca ccgtcggcca gcgcgccagg aagcgctcat
     3421 agtaggggat gaccgccttg accgtggtct gctgcagcat gatctcggac agccagatgc
     3481 ggtaggcatc gggcgcgcgc cccggcgccg tgcgccaagg cagctcgcgc cgctcgcggt
     3541 cccaccacgc cagcagcaga tcggcggtgt cggacccggc gggacatgcc gtgggttgcg
     3601 ctcttgcaac attcgccatg cggcctcgca ccccctgcgc ccgaatcagt cctctcatgg
     3661 taaggcgtca cccggtcccc tggaatgcgc cgaaggtgcc cccgtgacgt tgtcccccgt
     3721 gcgaggctct cccgcccacc ccctgccgcg ccaagcgctg gcttttgccc gccccactgc
     3781 gcgcagcctc aaggtcaagg cggtgggcag cttcctgccc tcgctcacgg cgcaagcctt
     3841 caagaagttc ggattttcca ccgcgcagct catcatggat tggccggcga ttgccggcaa
     3901 ggacttggcc gcccattcgc tgcccgagcg cctgaaatgg ccgccgcggc ccgaagccga
     3961 ggctgaggag ggcgagccgg cgcaaggccg gcgcggggcc acgctgcttt tgcgcgtcaa
     4021 tggcgcgcgc gcgctcgaaa tcgactacca gcgccagcag atcctggagc gcgtgaacgc
     4081 ctattttggc tatcgcgccg tgagcgagtt gcgtctggtg caggcgccgc tgccagccgc
     4141 gccgaccgcg cccgtgcgcc cgccgcgggc ggcggcgtgc ccgccgccat gcacggaggt
     4201 ggtgggcatc cctgacgcgg ccctgcgcga ggcgctggcg cggcttggct cccgcctggg
     4261 cgcgcgattg gccccccgct gagccctaag ggtcgccgcc ttcacgcgga tttgccttat
     4321 tttccggcga tcctatgtta tcaggcgaga gcgcgggagc gccggctggg cgccagcact
     4381 ggcgcttgcg cttttgccac gcacgattga gcgcacgagc ttgccccgca cggggagaga
     4441 gttcgcttca ggaaaccgat ctttaggcgg cgacacgcga cgtgaggagt gagcccttga
     4501 acgcctttga gcccatcaac gtgcaccggg ccctgtcccg gcgcgcgact ttcaagttcg
     4561 ctctcggcct tgcggtcggc gcggcggggg tgtcgtgggg gttggcggcc gccgcccaga
     4621 aacgcgccca aggacgcgcg gaaattccca tcgacgagct gatgcagccg ggcccgctgc
     4681 ccgagttggt gctgggcaag gaggatgccc cgatcaccgt cgtggagtat gcctccatga
     4741 cctgcggcca ctgcgccaat ttccacaaca aggtgtttcc gacccttaag gaaaagtaca
     4801 tcgacagcgg caaagtccgc ttcgtcatgc gcgagtttcc cctcgacaag ctggccgtgg
     4861 cggcatcgat gctggcgcgc tgcgccggcg gcgaaaagac cttccccctg atttcggcgc
     4921 tgtttgccaa acaggaggaa tgggccttcg tgcgcggcaa cccggtgccg gagcttttca
     4981 agctcgccaa acaggcgggc tttacccagg aaagcttcga taagtgtttg accgatcagg
     5041 cactcatgga tgacatcgtc aagatcaggg gacgggctgc agatgtcttc ggcgtcggct
     5101 cgacgcccac gttcttcatc aatggcaaga agatgaccgg cgcgccgacg gtcgaagagt
     5161 tcgacaaggc cttcgcggcc ctcttgaaga gctgatggcg cgccgctcaa cgccgatgca
     5221 gaaccgatca agcgctgacc tcgaggcttg agtgccgcca cgccgcgctc ttctggcttt
     5281 ttgcctcgcc gcccagctgg cggggtgcag cggcgacgtg ccgttgctgg gcgagccgcc
     5341 ccccaccggg gccgcgcttg aggcaggtgg ggccaccgtc gatcggccgg ggttcgatcc
     5401 ctttcaggcg acggtccccg ggccggcacc cagccgccag atcctgcaaa atcccacgct
     5461 gggagacatc ctgaaaaccg gcgagctgcc cgagatggcg cttgggcgca aggatgcgcc
     5521 tgtgaccatt gtgcagtatg cgtccatgac ctgcccctac tgccggcgct tccaggcgga
     5581 aacctttccc gtgctcaagc gtgagtacat cgacaccggc aaggttcgct acgtgctgcg
     5641 cgcggagttt ccaatcggca agcagtctgg gctcgccacc attgcgctga ggtgcgcgcc
     5701 gccggagcaa tatttcgtgc tctacgacaa gctgatgtct cagcaagcca cctgggtcag
     5761 ccaggaagtg cggcccgatc caatcttcaa ggtcgccgca caggtgggga tgacgcgtgc
     5821 acagtttgac agctgtcgcg agaatcgcgc catgatcggc caactcaact ggatcaagga
     5881 gcgcggccgg acgctcggca tcatcgggac gcccaatttc ttcattcagg gcaaattggt
     5941 gaagtcggtg ctcggcatga aagagatccg cgagatggtg gatcccatgc tggccggcca
     6001 agtggccgtt tccgggccga gggaaggagc ccgctaggct cgcggcgcgg caatttctgt
     6061 tgcgtttggt gcccggcacg ggccaagagc ttgcccggca ggggttgcgc acgttttttg
     6121 agggtgcctc tcgtcatgca tatcaaccgg ctgaggctgt tgggcttcaa gtcgttcgtg
     6181 gagccgacgg agcttctggt cgagccaggc ctgaccgggg tcgtggggcc gaacggctgc
     6241 ggcaagtcca acctgctcga ggcgctgaga tgggtgatgg gcgagagttc gcacaaaagc
     6301 atgcgcgcgg cggccatgga cgacgtcatc ttttcgggca ccgccgaccg cccggcgcgc
     6361 aataccgcag aagtgacgat tttcctcgac agcgcgggca acaagctgcc ggccgaattc
     6421 catgcgggcg agacgctcga ggtcacccgt cgcatcgaac gcgaggcggg ctccgcctat
     6481 cgcatcaatg gtcgcgaggc gcgcgcgcgc gatgtgcgca tcctgttcga ggacgccgcc
     6541 accggggccc gctcgccggc cctggtgcgc cagggtcaga tcggcgaact cgtcaacgcc
     6601 aagccggagc agcgccggcg cattctcgag gatgcggcgg ggattgccgg cctgcacacg
     6661 cgtcggcacg aggcggaatt gcgcctcaag gcggcggacg ccaacctggc ccgcatccag
     6721 gacattctgg gtcagctcta ttcgcaaatc gagagcctga agcggcaggc gcggcaggcg
     6781 cggcgttaca aggacctttc cagcgagatc cgcaagggtg atgccctcgt cctgcatttg
     6841 agctggcagg aggcccagac ccaggtcagt accggggaaa ccgcgctggc cggcgccttg
     6901 gaaaagttgg cgctggcaac cgagcttgaa tcgaaggcgc tgcgggccga ggccgacgcg
     6961 gccggccggc tgccgccgct gcgcgatcaa gaggccgcca aaagtgcggt gctgcaccgc
     7021 ttcaaagtcg agcaagacaa cctcgagcgc gaggcgcggc gcaccgagga acgctgccag
     7081 gagcttgagg cgcgtagcga gcagctcagg cgcgatctgg agcgcgagga cctcctgcgt
     7141 cgggaggccc aggagacgct ggcgcggctc gaggcggagc gtcaatcgct cgccaacacc
     7201 gccgctttgg cggccgactt cgagcaaaag gcgctcgaga gctttgaagc aggcgatgcg
     7261 cgccttgccg aggcggagcg ccggctgcaa cacctgacga cggcggccgc cgaggcgcgc
     7321 gcgcgtcgcc aaagcctcga ggcacagcgc caggagcgcc aggggcaaat cgaaaagctc
     7381 acctcccagc tggtcgcgct cgaggcgcag acgcgcgaga tcgtcgggcg agcgccggat
     7441 gcctccaagc tcaaagttac ggccgaggcg ggacagcgct tgatgaccga gatcgccgtc
     7501 atcgaagcgc aggcgttgac cgccgaggag gccgcacagg ccgccgcgct cgaggcggaa
     7561 cagaaaagcg agacggcacg cgcagccgat cttgccgccg gcaaactgaa gaccgaggtc
     7621 gagacgctga aaaagctgct caagccctcc gatgccggcg gtttgccgcc catcctcgac
     7681 gagatccgcg tggccgccgg ctacgaaatg gcgctggcgg cggcgctggg cgatgacctt
     7741 gaagccccgg cggtcgaagc cgcggcgctg cactggcggc tcaacccggg cgacgagcgc
     7801 gaccctgccc tgcccgccgg cgccgagccg ctgagcgcct atgtcacggc gccgccgcag
     7861 ctggcgcgac ggctggccca gatcggcctc gtcgagcaaa gcgagggcgg ccgcttactg
     7921 cgccaactgc gcagcggaca acggctcgtc tcgcgtcagg gcgatctatg gcgctgggat
     7981 ggctatgtgg cggctgccca gggcaccagc gcggcggcga gccgcctcgc cgagcgcagt
     8041 cgcctcggca tgctggaagc cgaagaggcg agccaacgcc aaatcgcgga ggtggcgcgt
     8101 gctgcgtccg cagccgccga ccagcgccat aaggctgccc agacggagga gcgccggctg
     8161 cgccagcttt ggcgcgagac gcagggccag ctggctcaga cccgcgaggt cctgaccggc
     8221 ttggagcgcc aggcgcggga aacggaggcc aagttggcgg ccgtcacggc ggccaagcag
     8281 aagagcaccg aagccctcgc cgaggtgcag gatttgttgg ccaagagcga aaacgccctg
     8341 ggggcgctcg cgggcattga ggcgctcgaa gccgaactgg ccgcggcaca aacagacacc
     8401 aacggcctgc gcggccaagt ggcggagacc aagacggaac tcgtcacctt ggagcgcgag
     8461 caccgcgcgc gcgtcgagcg gcagaaggcc atcgggctcg aaaacgagcg taacctggcg
     8521 cgcatcgcca gcgccgaaga gcagatagtg gcgctgaagg accgcgcggc cggtaccgac
     8581 gccgaacttg cgcgcttggc cgacattcca cgcatggtcg agcagcagcg ccagacgctg
     8641 atgacggaac ttgctgccgc cgagcacgat cgccgcaacg ccgccgacac gctggcggcg
     8701 gcggagacga cgcaccggca ggcggcgcaa gacctgcgcg cggcacaaac cgccgtcgcc
     8761 gacgaacgcg aacgccgggc ccgcagcgag gcgcacctgg agaacgcgcg ccagcgccgt
     8821 gccgaggagg gccgcaagat tcgcgatcaa ttgggctgcg cgcccgagga ctgcctggcg
     8881 ctggccggct tggcggggga agccgcgctg ccgagcctcg aggcggcgct cggcgggctc
     8941 aatcgtctca aggccgagcg cgaacgcctg gggggcgtca atttgcaggc cgatgacgac
     9001 ctcgccgggc tcgcgcagca gttcgaaagc ctggacaagg agaagcgcga cgttgaagcg
     9061 gcgatcgcca agctgcgtgc gggcattcag cagatcaaca atgaaggccg tacgcgcctg
     9121 caggccgcct tcgatgccgt caatggtcac ttcaagcagt tgttcatgac gctgttcggg
     9181 ggcggggagg cccgcctgga aatgatcgat gccgaggatc ccctcgatgg cggcctggag
     9241 atcatcgcca agccgccagg caaaaagccc gcgaccttgt cgctgctctc cggtggcgag
     9301 cagtcgctga cggcgatggc tctgatcttc gccgtgtttt tgaccaatcc gtcgccgatt
     9361 tgcgtgctcg acgaagtcga cgcaccgctc gatgatgcca atgtcgaccg cttctgcacc
     9421 ttgctggaga agatggcggc cgatacggcg acgcgcttcc tggtgatcac ccaccatccg
     9481 atgaccatgt cgcgcatgaa ccggctgttc ggcgtgacca tggccgaaaa gggcgtgagc
     9541 cagctcgtgt cggtcgacct tgaaacggca cagaagtttg tggtggcggc ctgagcgggc
     9601 agcaaaggtg ggctaggccg gtgcacgtgc tcctcgacct cgacgggacc ttgctcgacc
     9661 ccggcgccgg cctgatcgct tccatccaat acgcgctcaa ggcgtcgggc aagccggtcc
     9721 cgccggcggc cgagctcggc tggatgatcg gtccgccgtt gcgagcctcc ttcgcggagc
     9781 tgctgggcgg gacggagccg gcggaggcgg cgctcgcgca ttaccgcgcc tatttcgggg
     9841 cgcgggggat gtacgaggcc accgtctatc cgggcattcc cgaggcgctc gacgcgctcg
     9901 ctggcgcggg ctgtcgcctg atcgtagcca cggccaaacc gcatcacttc gcccgcccga
     9961 ttctcaagca ctttgcgctg gccgaccggt ttgccgccat tcatggcccc gagcttgacg
    10021 gtacgcgcga tgccaaagcc gacttgatcg ccttcatcct cgaccgggaa ggggtggccg
    10081 tcgacgccgc gctgatggtg ggtgatcgcg ctgtggacgt gcgcgccgcc cgcagcaatg
    10141 gcatgcgcag cgtcgccgcc gcctggggtt acggcacgcc caccgaactc agcgcagcgg
    10201 gagcggcggc catttgcgag acgccgcagg ccttggcgcc cctcgttttg gcgctgcttg
    10261 gcgcggagcg ccgcccacgc tgagggccgc caggccgggc gcaaggggca cggtccaatt
    10321 gtcgcatcgc tcatcgggtg gcgctgccgt ggtgaccgcg ccgcgcgcgg ccgccgccat
    10381 attcttgaca ctcgcgggaa ccgctgcttt aaggtccccg cgctttgata ggccccgggg
    10441 ggagtcgggc cgcctcccac cgaggacgtg atcctcatgc cagatccaac ggatcatcga
    10501 ggtcctggtg aacgccagat gagccccgag gaggcggctg cgttccaagg tcgggttgcc
    10561 gagctcggcg accgcctcgg taagatcaag gcgcaacggg atgccgatgc gcatgccgat
    10621 ctcgatgcgg aaatgcgcgg ccgcggcatg gcctatggga tgcgcatggc cgcggaaatg
    10681 gtcggcgcgg tcatagtcgg aggtggcatc ggctggggtt tggactggtg gctcggcagt
    10741 aaaccttggc tgttcctgct cttcttcctg ttggggtttg cggccggcgt cctcaacgtg
    10801 gtgaggggct acgagcggat gcagaaggac ttcatggcac gcacgggcgg ccggcttggt
    10861 cacagcgtgc cggacgacga ggactgagga gcttagcgtg gcagcgccaa atgcgggaac
    10921 cgggggcacg gggatgcccg acccgatcca ccagttcgag atccatcgca tcatccccat
    10981 caagatcttt ggttgggatg catccttcac caacgcctcc ttgttcatgc tgctggcggt
    11041 gctgctgatc gccggctttt tcgtggtctc catgcagacg cgctcgctgg tgccgagccg
    11101 cctgcagtcg ctcgccgagg tcagctacga attcgtcgcc ggcatgctgc gcgaaagcgt
    11161 tggccacgct ggcatgaaat acttcccgtt cgtgtttacg atcttcatct tcgtcttcac
    11221 cgccaacacc ttgggcctaa tcccgggttt cttcaccgtc accagccaca ttgtggtgac
    11281 ggcggcgttg gccgctatgg tgttcctcac cgtcctggtg gtgggcttcg ccaaaaacgg
    11341 cgtgaagttt ctgcagctgt tcgtgccgca gggtgtaccc attctgattt tgcctctcgt
    11401 cgtgctgatc gagatcatct cgtttctgtc gcggcccgtg agccactcgg ttcgcctgtt
    11461 tgccaacatg ctggccggcc acatcacgct caaggtgttc ggtggtttcg tggtgatgct
    11521 gctcggtgcc ggcacctacg cggcgctggc accgctgcct ctcgtcatgg cgatcgcgct
    11581 caccgcactc gaggtgctgg tcgctttcct gcaagcctac gtgttcacca tgcttacctg
    11641 tatgtatctc aacgatgcgc tacatccggg gcactagatc gaagccttcg gtctgaagac
    11701 ggtttaactt acaaaggaga acagaagtcc atggatcccg cagccgcaaa actcatcggc
    11761 gccggccttg ctgccatcgg caccggtgct gccgcgatcg gcgtcggcaa tctttttggc
    11821 cagtttctgc agggcgcgct tcgcaatcca tccgctgccg atgggcagtt cggccgcctg
    11881 atctttggct tcgccgtcac cgaagcgttg ggcattttcg cgctgctgat tgccttcctc
    11941 ttgctcttta cctaaagccg ggcggcagcc aggatagatc gcgatggcga ccaacagcac
    12001 agccggcacc acgaccgccc acgcgacggg gcagccgaag gtattcccgc cgctggactc
    12061 gggcaccttt gccccacagc tgatctggct ggcgctcagc ttcggcttgc tctatctgct
    12121 gctcaagcgc ttttatttgc cgcg
//
LOCUS       DASTMT010000504        11186 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_2257363, whole genome shotgun sequence.
ACCESSION   DASTMT010000504 DASTMT010000000
VERSION     DASTMT010000504.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 11186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11186
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2257363"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..603
                     /locus_tag="VFR00_11580"
     CDS             <1..603
                     /locus_tag="VFR00_11580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0159952.1"
                     /translation="AEQQGARGLFGDRPDGARALRTLSREGTVEGGRASFRLNQVYAG
                     AEHYVLLELELDQGLTAAVGERVLGVVTVSYWQPSSQSRQTLTTPIRVSISASKEAVA
                     AGRDRKVEEAVLEQVTRQRAQQAIKLKDDGNAGEARQLLLQNSKELEAFLATAPTAPA
                     RLLELNKQYQALTAPAGKAASERKVLRALEIPAAGSATRY"
     gene            complement(867..1838)
                     /locus_tag="VFR00_11585"
     CDS             complement(867..1838)
                     /locus_tag="VFR00_11585"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEU0159953.1"
                     /translation="MRSGRWLLVLLTAWVLIGPALAQTYPERAIKIIVPQPPGGGFDT
                     VARVLADRLSPLLGQPLLVENRPGAGTLVGTEAAAKAPADGYTLLLGALSNIALNPGL
                     YDRLAYDPLRDFVPVGLAVTWSYTLVARKDLPHKDLNDLVAFARANPEQVTYASAGRG
                     SGQHIAMAVTAQLAGIKLTHVTYRGAQAAYQDVLGGRVDLFFDISSTARAQVDAGSVR
                     ALAVSSRARQPMHPQVPSVAETGVAGLDMESWFGLFAPAAVPAATLARLRVELSKVLA
                     DPEVAALFANTGGRLLNLSPAETEALIKRDVERWTKLIREAGISASQ"
     gene            1940..2809
                     /locus_tag="VFR00_11590"
     CDS             1940..2809
                     /locus_tag="VFR00_11590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEU0159954.1"
                     /translation="MALFKLRIEATDADAGRTAAGLLGELVAPEPLAVTLFEARPPRF
                     TVEAYWDTEPPMARLAQALADVVGLGAPALEAVPDLNWVALSQAALPPIEAGRLLVHG
                     SHDRARFGLRQTAIEIDAGEAFGTGHNPTTAGCLVALDALSRRRSFRRVLDLGCGTGV
                     LAIAAARLMAHAQVFAVDNDPIATAVARANARLNRTGRRLTILNANGFAHPSLRRARP
                     FDLVLANILPRPLVRLAAPMRRTIAVGGFAVLSGLLNHQAREVAMAYRAAGFRLAGWE
                     RRAGWSILTLQRS"
     gene            complement(2827..4374)
                     /locus_tag="VFR00_11595"
     CDS             complement(2827..4374)
                     /locus_tag="VFR00_11595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008138683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HEU0159955.1"
                     /translation="MNGAESLVRTLLAGGVDVCFTNPGTSEMHFVAALDKVPGMRCVL
                     GLFEGVVTGAADGYYRMRGQPASTLLHLGPGLANGLANLHNAKKANSGIVNIVGEHAT
                     YHIAHDAPLTSDIEGLAAPMSHWVKTSPNAASLASDGALAIAAARQTPGQIATLILPA
                     DTAWTAADGIAEPAPSAPVRSVPPAAIEAAAAMLRSGARATLLLGGPALRQRSLEWAG
                     KIAAKTGCGLLTEGANARLERGAGRVQVERIPYVVDKALAVMRAAGNLVLVGARQPVA
                     FFAYPDKPSLLMAEGTRASTLAGLDEDIEAALEALAAELGATAMAPTGLAAPRRPALP
                     GGAITPAAIASILGAMLPENAIVVDESVSTGREFFPETAGAPPHDWLNNRGGSIGYGM
                     PVAIGAAIACPERKVIALEGDGSAMYTLQSLWTMAREGLDVTVLILANGSYNILRGEL
                     TNVGVKNPGPRAVDMLSLRRPDLDWVSLARGMGVEAIRAKDCEQLSKALSAGLRSGGP
                     YLIEVAI"
     gene            complement(4568..6898)
                     /gene="bamA"
                     /locus_tag="VFR00_11600"
     CDS             complement(4568..6898)
                     /gene="bamA"
                     /locus_tag="VFR00_11600"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HEU0159956.1"
                     /translation="MHFRVHCRLWKSRVALAVLALVYLLPGRVLAADSIGAVEVVGNR
                     TVDAASIRSHLHPGNRGTVTPAQIDEALKALFATGLFSDVRIDRKGGKLVISVTENPV
                     IASVAFEGNANADKSKLEPLLVLKARARYTAAKAHADALKVRDFYRSQGRLATEVEPK
                     LKERTDGQVALVFAIKEAAVTKVDNIVFMGNRSFTDRQLRDVITTSQSGWFDILKTAA
                     FYDPERIKQDQDLLRQFYLKNGFPDVRVVTAEATKNEQGTGYAIAFTIEEGERYAFGP
                     TTIQSSIAKVDTASLQSSVTIKPGATYSAEQVEKSVERMTLMLSDQGHAAANVRVVPK
                     RDEASRTITMAIEVKEGTPVYIERIEVAGNSQTKDFVIRRELRFAEGDVVNAFVLERA
                     RKRVQALGFFKKVTLKRKLGSSPDRVIVTVEVIEDDTRNIAFGAGYSSSEGIVGDIAV
                     TERNLFGNGQALRVKLAGSTTRLQAELGFTEPRLFGSNFAGGFDLFYKDTDYTTQSSF
                     KSQRVGGDLRLGYQINDEWSGSVNYTFSRNTIYDVGANASAAIKEAVPGFPNVSSNTY
                     DTSSVGYSLAYDTRNNKRRPTEGVYYTLSQDLAGVGGDVRYLRSTGEARGYYPVSDTI
                     TAAGRVTGGVVGGWGGQDVRLLDLFYRGSESVRGFAYSGIGPRDILSANKDALGGRMY
                     FATTAELLFQIPGLPQDFGLRGAVFADAGSLWGVNKTAASLPGLAGNSLAPRASVGVG
                     LAWDSPLGALRADYAFPIVKQPFDKTQAFSFGLSPF"
     gene            complement(7299..8024)
                     /locus_tag="VFR00_11605"
     CDS             complement(7299..8024)
                     /locus_tag="VFR00_11605"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0159957.1"
                     /translation="MSQQVKAELAPTGVLRAGINLSNFLLVTGKTEAGDPVGVAPDMA
                     SEIARRLGVPVRFVTYKTPGELADMADTGAWDIGLIGAEPQRAEKISFTPAYVEIEAT
                     YLVPAGSPLKSIADVDRPGVRIAVTGRSAYGLWLDRNIKQAELVKSGTLDSAYEQFVS
                     DKLEALAGLRPRLLSDVEKLPGARILDGQFTAVQQAVGTARRNSAAAEYLRNFVAEMK
                     ASGFVGRLIEKHKVRGLSVAPPA"
     gene            complement(8309..9844)
                     /locus_tag="VFR00_11610"
     CDS             complement(8309..9844)
                     /locus_tag="VFR00_11610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011313898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU0159958.1"
                     /translation="MGSAQGQPAGPDLTLGIASEELGDGLMLAGHVGDSAVLLARCGN
                     EIFAVDAKCSHYGGPLADGLLVGATVRCPWHHACFSLASGEAVDAPAFSPLACWEVEE
                     RDGRLFVERKKATEPERETAPRSATAPDRIVIVGGGAAGFAAAEMLRRLKYQGAVSVL
                     SADDAAPVDRPNLSKDYLAGQAPEDWLPLRPDAYYAEQGIHLRLQANVMAVDLGAKAV
                     VLAGGERVAYDRLLLATGAEPVRLSIPGANLPHVHVLRSLADCKSIIATAAAAKRAVV
                     LGASFIGLEVAASLRGRGLEVHVVAPEALPMQRLLGPQLGEVVRSLHLEHGVVFHLPD
                     VATAITGKHVELKSGTRLQADLVVAGIGVKPRLELAQQAGLPIDRGVLVDGRLETGAP
                     GVFAAGDIARWPDPRGGGSLRVEHWVVAERQAQVAARNMLGLGQTYTAVPFFWSQHYD
                     VAINYVGHAERWDEVDVAGDPAAKDCLVRYRLGGRTLAVASINRDVASLEAELAMERE
                     GRA"
     gene            10056..10733
                     /gene="folE"
                     /locus_tag="VFR00_11615"
     CDS             10056..10733
                     /gene="folE"
                     /locus_tag="VFR00_11615"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016840697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HEU0159959.1"
                     /translation="MNGLATDIEPLIAAAAPLSAARPTARDRPTRAEAERAVETLIRW
                     AGDDPGREGLLDTPARVVRAYQQWFAGYQQDPAQHLRRTFEEVAGYDEMVVMRDIDFQ
                     SHCEHHMAPISGRVHIGYLPRNKVVGISKLVRLVEVFAGRLQTQEKLTAQIADTLYSV
                     LAPHGAAVVVEARHGCMTSRGVRQPSATLLTSRMLGVFRDRPETRQEFLAAVDLDGTK
                     RAGVARL"
BASE COUNT         1861 a         3819 c         3708 g         1798 t
ORIGIN      
        1 gcagaacagc aaggagctcg aggccttttt ggcgaccgcc ccgacggcgc ccgcgcgctg
       61 cgcaccctgt cgcgggaggg cacagttgag ggcgggcgcg cgagcttccg tctcaatcag
      121 gtctatgccg gcgccgagca ctatgtgctg ctggagctcg agctggacca gggcctgacg
      181 gcggcggtgg gcgagcgggt gctcggggtc gtcactgtca gctattggca gccgagcagc
      241 caatctcgcc agacgttgac cacgccgatc cgcgtcagca tcagcgcttc gaaggaggcg
      301 gtggctgcgg ggcgcgatcg gaaggtcgag gaggcggtgc tggagcaggt gacgcgtcag
      361 cgcgcgcaac aggccatcaa gctcaaggat gacggcaacg ccggcgaggc tcgacaattg
      421 ctgctgcaga acagcaagga gctcgaggcc tttttggcga ccgccccgac ggcgcccgcg
      481 cgtttgctcg aactcaacaa gcagtaccag gcgctgacgg caccggccgg caaggcggcc
      541 agcgagcgca aggtcttgcg cgcgctggaa atcccagcgg cgggcagcgc cactcgctac
      601 taagcgccaa cgcgcctcct cggccgcatg ctgttggcgt gaacgggccg atttggacgc
      661 ccgttgtaag gccaccaggg cgaggctggt ggcccggcgc tgccaccggc ctcgtctcgc
      721 ggtgtgcgcg cggggacacg tgcagggcgc gcgcaaaggc acgttcataa gtgcattcat
      781 gaaagcgggt tcaggctgtg cctgcctggc ctgcatgaag cgcctgcggg gggcgccttg
      841 cacgggcctc gagcacccgc gaggcctcat tggctcgcgc tgatgcccgc ctcgcggatc
      901 agcttggtcc aacgctccac gtcgcgcttg atcagcgcct cggtctcggc cggcgacagg
      961 ttgagcagcc ggccgccggt gttggcaaac agcgccgcca cttcggggtc ggccaggacc
     1021 ttgctcagct cgacgcgcag tcgcgccaac gttgccgccg gcacggctgc cggcgcaaac
     1081 aggccaaacc agctttccat atcgagcccc gccacgcccg tctccgccac gctcggcacc
     1141 tgcggatgca tgggttggcg cgcccttgag gagaccgcca acgcccgcac gctgccggcg
     1201 tcgacctgcg cgcgggcggt ggaggagata tcgaaaaaga gatcgacgcg cccacccagt
     1261 acatcctgat aggcggcctg cgcaccgcgg taggtgacgt gcgtcagctt gatgcctgcc
     1321 agctgcgccg tcacggccat ggcgatgtgc tggcccgagc cgcgtcctgc cgacgcgtac
     1381 gtgacctgct cggggttggc ccgggcgaag gccacgaggt cgttcaggtc cttgtgcggc
     1441 agatctttgc gcgccaccag ggtgtaggac catgtcaccg ctagcccgac gggcacgaag
     1501 tcgcgcagcg gatcataggc gagcctgtcg taaagccccg ggtttaaggc gatgttggag
     1561 agcgccccca acagcagggt gtagccatcg gcaggcgcct tggccgccgc ctcggtaccg
     1621 accagcgtgc ccgcgcctgg ccggttttcc accagcaacg gctggccgag cagcggggat
     1681 aggcgatcgg ccaggacgcg ggccaccgta tcgaagccgc cacccggcgg ctgcggcacg
     1741 atgatcttga tggcccgctc cgggtaggtt tgcgccagcg ccggccctat caacacccac
     1801 gccgtcagta aaaccagcag ccagcgccca gatcgcacgt ttgcccctcc ccaacttgac
     1861 ccgccgcttt cgcgcggccg cggaaacgag tatgaccgcg catcgacgtc gcgcacaacc
     1921 gagccagcct ccggcgaaca tggcactttt caagcttcgc atcgaggcca ccgatgccga
     1981 tgccgggcgc accgcggcgg gcctgttggg cgaactcgtc gcgcccgaac cgctcgccgt
     2041 tacgctgttc gaagcccggc cgccgcgctt cacggtcgag gcctactggg acacggagcc
     2101 gccgatggca cggctggcac aagcgctcgc cgatgtcgtc ggtctcggcg cccctgcgct
     2161 ggaagccgtt cccgacctca actgggtggc cttgtcgcag gcggccctgc cgccgatcga
     2221 ggccggccgt ttgctggtgc acggcagcca cgatcgcgca cgcttcggcc tacggcaaac
     2281 cgccattgag atcgatgcgg gtgaggcgtt cggcaccggt cacaatccga ccacggccgg
     2341 ttgtctcgtt gcccttgatg ccctgagccg gcgcaggagc ttccggcgtg tcctcgacct
     2401 gggctgcggc acgggagtgc tcgccattgc cgcagcgcgg ctcatggccc atgcccaggt
     2461 gttcgcggtc gacaatgatc cgatcgccac ggcggtcgcg cgcgccaacg cgcgcctcaa
     2521 ccgaacgggg cgtcgcctca ccatcctcaa cgccaacggc ttcgcacacc cgagcctgcg
     2581 ccgagcgcga cccttcgatc tcgtgcttgc caacattttg ccgcgtccgc tggtgcggct
     2641 cgccgcgccg atgcgccgca ccattgctgt cggcggcttt gccgtcctct caggcctatt
     2701 gaaccatcag gcccgcgagg tcgccatggc ctatcgtgct gcgggctttc gtctggcggg
     2761 ctgggagcgg cgcgccggct ggtcgatcct gacgctgcag cgcagctgac cccggcgacg
     2821 aggtggctaa atcgccacct cgattagata aggcccgccg gaccgcaggc ccgcgctcaa
     2881 tgccttgctc aactgctcgc agtctttggc gcgtatggcc tccaccccca tgccacgggc
     2941 aaggctcacc caatcgaggt ccgggcggcg gagcgaaagc atgtccaccg cgcgcggccc
     3001 cgggttcttg acgccgacgt tggtcagttc accgcgcaaa atgttatagc tgccattggc
     3061 aaggatcagc acggtaacgt cgagcccttc ccgggccatc gtccacagcg actgcagcgt
     3121 gtacatggcg ctgccgtccc cctccagcgc aatcaccttg cgctcgggac aggcgatggc
     3181 ggcgccgatc gccacgggca tgccgtagcc gatcgaccca cccctgttgt tgagccaatc
     3241 atgcggcggc gcgccggcgg tctcgggaaa gaattcgcgg cccgtgctga cgctttcgtc
     3301 aaccacaatg gcattctccg gcagcatggc gccgaggatc gaggcgatgg cggcgggcgt
     3361 aatggcgccg cccggaagcg ccggccgccg cggcgcggcc aagccggtgg gtgccatcgc
     3421 ggtggccccg agctcggccg ccagcgcctc aagcgccgct tcgatgtcct cgtcgaggcc
     3481 cgcgagcgtg ctggcgcggg tgccctcggc catcaacaaa ctgggcttgt cgggataggc
     3541 aaaaaacgcc accggttggc gggcgccgac cagcacgagg ttgcctgcgg cgcgcatgac
     3601 ggcgagcgcc ttgtcgacga catagggtat gcgctcgacc tgaacgcgcc cggcgccgcg
     3661 ctccagtcgg gcgttggcgc cctcagttag caggccgcag ccggttttgg cggcgatctt
     3721 gccggcccat tcgagagagc gctgacgcag ggcggggccg cccagcagca aagtggcgcg
     3781 cgcgccggag cgcagcattg ccgcggccgc ttcgatggcg gcaggcggaa cgcttcggac
     3841 cggcgccgac ggcgcgggct cggcgatgcc gtcggcggca gtccaggccg tgtcggcggg
     3901 caaaatgagg gtggcgatct gcccgggcgt ctgccgcgcg gcggcaattg ccagcgcccc
     3961 atcgcttgcc aggctcgccg cgttgggcga ggtcttgacc caatgcgaca tcggcgcagc
     4021 cagaccctcg atgtcggagg tgagtggggc gtcatgggca atatgatagg tggcgtgctc
     4081 gccgacgatg ttgacgatgc ctgagttggc cttcttggcg ttgtgcaggt tggcgagccc
     4141 gttggcgagg cccggtccca agtgcaacaa ggtcgaagcc ggctgcccgc gcatgcggta
     4201 gtaaccgtcg gcggcacccg tgaccacgcc ttcgaatagg cccagcacgc agcgcatgcc
     4261 cggcaccttg tcgagggccg caacaaagtg catttccgag gtgcccggat tggtgaagca
     4321 gacgtccacg cccccggcaa gcagggtgcg caccaggctc tccgccccgt tcatgctgcc
     4381 catggctgtt tgacgctcca tcgtgacatc gcgctggagg tcgcctgtaa tcggcctacc
     4441 cttgagagca ccgacccgcc tccgccgggg cgccatatca tcgttccggg tcggcatgaa
     4501 agaaaaacac cgccgccgtc gagatcttgg tggcacgccg cggcgcgccc cgctaaaccg
     4561 gcgccgccta gaacgggctc aggccgaagc tgaaggcctg cgtcttgtca aagggctgct
     4621 tgacgatcgg gaaggcatag tccgccctca gcgcaccgag cggtgaatcc caggccaagc
     4681 ccacgcccac ggacgcgcgc ggggccagcg aattgccggc aagacccgga agcgatgccg
     4741 ccgtcttatt gacaccccag agcgagccgg catcggcgaa tacggcgccg cgcagaccga
     4801 aatcctgcgg caggccgggg atctggaaca acagttcggc ggtcgtggcg aaatacatgc
     4861 gcccgcccag cgcgtcttta ttggcgctca ggatatcgcg cggtccgatg ccagaatagg
     4921 caaagccccg cacgctctcg ctgccgcgat agaacagatc gaggaggcgc acgtcctgcc
     4981 ctccccaacc gccgaccacg ccgccggtga cgcgacccgc ggccgtgatc gtatcggaaa
     5041 cgggatagta gccgcgagcc tcgcccgttg agcgcagata gcgcacatcg ccgccgacgc
     5101 cggcgagatc ctgcgataag gtgtagtaga cgccctcggt cggtcgccgc ttgttgttgc
     5161 gcgtatcgta agccaagctg tagccgacgg aggaggtgtc ataggtgttg gaggaaacat
     5221 tgggaaaacc cggcaccgcc tccttgatcg cggccgatgc attggcgccg acgtcgtaaa
     5281 tggtgttgcg cgagaacgtg taattgacgc tgcccgacca ctcatcgttg atttgatagc
     5341 caagccgcag gtcgccgccg acccgctggc tcttgaaaga cgactgcgtc gtgtagtcgg
     5401 tatccttgta gaagaggtca aagccgccgg cgaaattgct gccgaacaag cgtggttcgg
     5461 tgaagccgag ttcagcctgg aggcgcgtgg tgctgcccgc aagcttgacg cgcaacgctt
     5521 ggccgttgcc gaagagattg cgttcggtca ccgcaatatc gccgacgatg ccttccgagc
     5581 tcgagtagcc ggcgccgaag gcgatgttgc gcgtatcgtc ctcgatcacc tccaccgtaa
     5641 cgatgacgcg atccgggctc gagcccagct ttcgcttcag cgtgaccttc ttgaaaaagc
     5701 ccaaggcctg cacgcgcttg cgggcccgct cgaggacaaa ggcgttgacc acgtcgccct
     5761 cggcgaagcg cagctcgcgg cggatgacaa agtccttggt ctggctgttg ccggcgacct
     5821 cgatgcgctc gatatatacc ggcgtaccct ccttcacctc gatggccatg gtgatggtgc
     5881 ggctcgcctc gtcgcgcttg ggcacaacgc gaacgttggc ggcggcgtgg ccttgatcgc
     5941 tcaacatcag cgtcatgcgc tcgacggact tttccacctg ctccgcactg taggtggcgc
     6001 ccggcttgat cgtgacggag gattgcagcg atgccgtatc gaccttggcg atgcttgatt
     6061 ggatcgtggt cggcccgaag gcgtaacgct cgccttcctc gatcgtgaac gcgatcgcat
     6121 agccggtgcc ctgctcgttc ttggttgcct ccgccgttac cacccgcaca tcgggaaagc
     6181 cgttcttgag gtagaactgc cgcagcaggt cctgatcctg cttgatgcgc tcaggatcgt
     6241 agaaggcggc ggttttcagg atatcgaacc agcccgattg actggtggtg atgacgtcgc
     6301 gcagctggcg atcggtaaag cttcgattgc ccatgaagac gatattgtcg accttggtga
     6361 ccgcggcctc cttgatggcg aacacgagcg cgacctgccc gtccgtacgc tccttgagct
     6421 ttggttcgac ctcggtcgcg agcctgccct ggctgcggta gaagtcacgc accttgaggg
     6481 catcggcgtg cgccttggcc gcggtgtagc gcgcccgcgc cttgagcacg agcagcggct
     6541 cgagcttgga tttgtcggca ttggcattgc cttcgaaggc gacgctggcg atcactggat
     6601 tttccgtgac gctgatcacg agtttgccgc ctttgcggtc gatgcgcacg tcggagaaaa
     6661 gcccggtggc aaacagcgcc tttagagcct cgtcgatctg ggcgggcgta accgtgcctc
     6721 ggttgccagg gtggagatgg ctgcggatcg aggcggcatc gaccgtgcgg ttgcccacca
     6781 cctccacggc gccgatgctg tcggcggcca atacccggcc cggcagcagg tagacgaggg
     6841 ccagcaccgc aagcgccacc cggctcttcc acaatcggca atgcacgcga aaatgcacgc
     6901 gaactcgcgt cccttgaatg tcgagggcgg cgctttcaac cctccctcga gcccagcctt
     6961 aagcgtttga tgtagattgt aatctgggcg ggattgggta agccgcgagg cctttcatgg
     7021 gcgccccctg ccggaaagcc gcccccgcgt cggccgccgc aggcctaagg cgcggctcct
     7081 ggcccgactt tcgggggcga caaagccctc ccggctcgga gcctcaggcc aaccgggcgt
     7141 cccgaggcgg cgacagacgc tgcccgcccc attttgctca atgctggcgc gacgccccct
     7201 tgaaaagccc tgcgccgcca atgctggaag ctcttgcagc tgaggccgac gcggccgccc
     7261 gggggtaaaa ccgtggcggc cccgtttggc atggccccct aggcgggcgg cgccacggaa
     7321 aggccgcgca ccttgtgctt ttcaatcaag cggccgacaa agcccgaggc cttcatctcc
     7381 gccacgaaat tgcgcagata ttcggcggca gcgctgttgc gacgcgccgt ccccactgcc
     7441 tgctgtacgg ccgtgaactg gccatcgagg atgcgcgcgc caggcagttt ttccacgtcc
     7501 gacaagagcc gcggccgcag ccctgccagc gcctcgagct tgtcggacac gaactgctcg
     7561 taggcactat cgagcgtgcc cgatttcacc agttcggcct gtttgatgtt gcgatcgagc
     7621 cagaggccgt aagcgctgcg accggtgacg gcaatacgca ccccgggacg gtcgacatcg
     7681 gcgatcgact tcaggggcga gcccgccggc accagatatg ttgcctcgat ctccacgtag
     7741 gccggggtga agctgatctt ttcggcgcgc tgcggctcgg cgccgatcaa gccaatatcc
     7801 caggcgccgg tatctgccat gtccgccagc tcgcccggcg tcttgtaggt cacaaatcgc
     7861 accggcacgc ccaggcgccg ggcgatttcg ctggccatgt ccggcgccac gccgacggga
     7921 tccccggcct ccgtcttgcc cgtcaccagc aggaaattcg ataggttgat gcccgcgcgc
     7981 agcacgcccg tcggcgccaa ctcggctttg acttgttgcg acatgagcgc ttcctcctgc
     8041 ttggccgctg ctgatggtga cgaggccgcc tgcggcaata gcccgacgag actgatgcga
     8101 tgagggcaat ccatctgatc acggcactac ctcgccacgg tcgagccggc actttaaccg
     8161 ctcatggtcg gcaaacgcca gcggcgagcg ccgggcctcg ttgccaagga gccgtttgtc
     8221 gccgatcaca gcgcgcttgc ggcacccgtg ggacgcgctg cggcagccgc cggatcgagc
     8281 cgcgggcggc gcagcagtcg gcgccaactc aggcccggcc ctcgcgctcc atcgccagtt
     8341 ccgcttcaag gctggcgaca tcgcggttga tcgaggcgac cgccagcgtt cggccgccca
     8401 accgatagcg caccaggcaa tccttcgccg ccgggtcgcc ggcaacgtcg acttcgtccc
     8461 aacgctcggc gtggcctacg tagttgatcg ccacgtcgta gtgctggctc cagaaaaacg
     8521 gcacggccgt gtatgtttgc cctaggccca gcatgttgcg ggcggcgacc tgcgcttggc
     8581 gctcggccac cacccaatgc tccacgcgca ggctgccgcc gcctcgcgga tccggccagc
     8641 gggcgatgtc gccggccgca aacacgccag gggcgccggt ttccaggcgg ccgtcgacaa
     8701 ggacgccgcg gtcgatgggc agcccggcct gctgggccag ctcgaggcgc ggcttgacgc
     8761 cgataccggc cacgacgaga tccgcctgaa gccgcgttcc gcttttcagc tcgacatgct
     8821 tgccggtaat ggcggtcgcg acatccggca ggtggaacac gacaccgtgt tcgagatgca
     8881 gtgagcgcac gacctcgccg agttgcggcc cgagcaggcg ctgcatcggc agcgcctccg
     8941 gcgccaccac gtgcacctcg aggccgcggc cgcgcaggga ggcggcaacc tccaggccaa
     9001 tgaagctcgc acccaagacc accgcccgct tggctgccgc ggcggtcgcg atgatgctct
     9061 tgcagtcggc cagcgagcgc agcacgtgga cgtggggcag gttcgcaccc ggaatcgata
     9121 gccgcacggg ttcggcaccc gtcgccaaca gcaaccggtc gtaggcaacc ctctcgcccc
     9181 cggccagcac caccgccttg gccccgagat cgacggccat gacattggcc tgcaggcgca
     9241 ggtggatgcc ttgctcggca tagtaggcgt cgggtcgcaa cggcagccaa tcctcgggcg
     9301 cctgcccggc cagatagtcc ttcgacaagt tcggacgatc gacgggcgcc gcatcgtcgg
     9361 cactgagcac gctaacggcg ccctgatact tgagccgccg cagcatttcg gcggcggcga
     9421 agcccgccgc cccaccgccc acgatgacga tgcggtcggg tgcggtcgcg ctccgaggcg
     9481 ccgtttcgcg ctccggctcg gtcgccttct tgcgctctac aaagaggcgg ccatcgcgtt
     9541 cttccacctc ccagcacgcc agcggcgaaa acgccggggc atcgaccgcc tcgccgctgg
     9601 cgaggctgaa acaggcatga tgccaagggc agcgcaccgt ggcgccgacc agcagcccat
     9661 cggcaagggg gcctccgtaa tgggaacact tggcgtcgac ggcaaagatc tcattgccgc
     9721 agcgcgccag cagaacggcg ctatccccga cgtggcccgc gagcataagc ccatcgccga
     9781 gctcttcgct cgcgataccc agtgtcaggt cgggcccggc cggctgtccc tgtgcagatc
     9841 ccatgacgac ctcctgggcg acctcgtggg tggcctcctg cgcgacctcc cgctccagcc
     9901 ccctcgtcgc ttggccgtgc tcggtcaggt agcatggcat agatgcacaa gcgcgccaaa
     9961 ggtttcccag tcccccaaag cacagaccga ggtgggaacc aatcgtggcc tcacacatcc
    10021 aatgccgcac aacccctaag gcgagagggc gagctatgaa cgggctggca acagacattg
    10081 aaccgcttat tgctgccgcg gcacccttga gcgcggcgcg gcctacagcg agggatcgtc
    10141 cgacgcgtgc ggaggccgaa cgggcggtcg agaccctgat ccgttgggcc ggcgatgacc
    10201 ccggccgtga agggctgctc gacacgccgg cccgggtggt gcgcgcctat cagcagtggt
    10261 tcgccggcta tcagcaggac ccggcgcagc atctgcggcg caccttcgag gaggtcgccg
    10321 gctacgacga gatggtggtg atgcgcgaca tcgattttca gtcccactgc gagcaccaca
    10381 tggcgccgat cagcggacgg gtccatatcg gctacctgcc acgcaacaag gtggtcggca
    10441 tttccaagct ggtgcggctg gttgaggtgt ttgccgggcg actgcagacg caggaaaagc
    10501 tgacggcgca aattgccgac acgctctata gcgtcttggc gccacacggt gcggcggtgg
    10561 tggtggaagc ccggcacggc tgcatgacca gccggggcgt gcgccagccg agcgcgacgc
    10621 tgctcaccag ccgcatgctc ggcgtctttc gcgatcggcc ggagacgcgc caggagttcc
    10681 tggcggcggt cgacctcgac gggacgaagc gggcgggtgt ggcccggctg taagcagcaa
    10741 ggcggcgtca tggaaaatag gccaatgccg aggttgggcg cccggccagc agcaactttg
    10801 ccacgggcgc gaagacgccg agctcgccga gggtgccgag ccgaggacat cgtcgagagg
    10861 tctatgccgt tgccatcggc aggccgcgcg cagttcgcgc cccgatccag ccttccacct
    10921 ggccccgagc tcgaaccgac agtgaccggc acttttgaca ttgctggcga acgctgtcgc
    10981 caaaggacgc aagcgcaacc gacgggcgcg gctcgccgcc ggcacaagcc gcctgagccg
    11041 gcacggcgcc ggcggacgga aaggaggggt agctcaagcc ctgactagga aggcgcccgg
    11101 cgttaggtca aggtcccgcc ggcgctaccc gacccttttg cccctaggct gcgcaaggct
    11161 cgcaattagc tcgctcaaca acgtcg
//
LOCUS       DASTMT010000505         2247 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_5395003, whole genome shotgun sequence.
ACCESSION   DASTMT010000505 DASTMT010000000
VERSION     DASTMT010000505.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2247)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2247
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5395003"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..683)
                     /locus_tag="VFR00_11620"
     CDS             complement(<1..683)
                     /locus_tag="VFR00_11620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEU0159960.1"
                     /translation="MTAMIKGAELKGGANYDVDTIRTDFPILFREVYGKPLVYLDNGA
                     SAQKPRPVLEAIEHAYKMEYANVHRGLHYLSNTATARFEDARETVRRFLNAATVEEII
                     FTRNATEAINLVASSFGGLTIGEGDEIVISVMEHHSNIVPWHFHRERRGAVIKWAPIS
                     DSGEFLLDRFEGLLGPRTKMVAITHMSNVLGTIVPIKEVIRIAHARGIPVLVDGSQAA
                     VHMTVDVRDL"
     gene            complement(717..2045)
                     /gene="sufD"
                     /locus_tag="VFR00_11625"
     CDS             complement(717..2045)
                     /gene="sufD"
                     /locus_tag="VFR00_11625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HEU0159961.1"
                     /translation="MTVAITRTKAEQGFTQNFEAAATQLPGGPAVAAARRAAIGAFAA
                     LGLPHRRLEAWKYTDLRSALKEALPAAVGAGRALPTAAQIDTALEAFAELDVHRLVFV
                     DGVYAQALSAQGALAGLSFAPLAAWLAKPEGVAADGLAAAAEQDAVVALNTAFMSDGA
                     VIDIARGAKLAKPLLLLFIRSAGEARLITTRNLLSVGAEASATVIEAHLSLPGAAAGQ
                     SNALSELAIAEGAELAHVKLTLAGGAESHLATWRPRLGAGASYRAFQFTAGTGLVRHG
                     LDLTFTGEGAKADVSGCFLGRANEHIDSTLVIDHAQAGCESRELFKGVLADRARGVFQ
                     GKVIVRPDAQKTDGKQMAQVLMLSEDAEFDSKPELEINADDVVCGHGSTSAEIDEDLL
                     FYFRARGIGKEEARALLIESFIGEAIDKVEAELRPALMAAARRRLAQLST"
     gene            complement(2117..>2247)
                     /gene="sufC"
                     /locus_tag="VFR00_11630"
     CDS             complement(2117..>2247)
                     /gene="sufC"
                     /locus_tag="VFR00_11630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009339364.1"
                     /note="part of SUF system involved in inserting
                     iron-sulfur clusters into proteins; in Escherichia coli
                     this protein forms a complex with SufBD; the SufBCD
                     complex stimulates the cysteine desulfurase SufS in
                     conjunction with SufE; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HEU0159962.1"
                     /translation="LNYIVPDRVHVMAAGKIQKSGGKELALELEKTGYAEFQSRAA"
BASE COUNT          360 a          762 c          722 g          403 t
ORIGIN      
        1 agatcgcgca catcgaccgt catgtgcacg gccgcctggc tgccgtcgac cagcaccgga
       61 ataccgcggg cgtgcgcgat cctgatgacc tccttgattg gcacgatggt acccagcacg
      121 ttcgacatat gggtgatggc caccatcttg gtgcgcggac cgagtaggcc ctcgaagcga
      181 tcgagcagaa attcgccgct atcgctgatc ggcgcccact tgatgacggc gccgcgccgc
      241 tcgcgatgaa agtgccaggg cacgatattg gagtgatgct ccatcaccga aatgacgatc
      301 tcgtcgccct cgccgatcgt cagacccccg aaagagctgg ccaccaggtt gatcgcctcg
      361 gtggcgttgc gcgtaaagat gatctcctcg accgtcgcag cattgagaaa acgccgcacg
      421 gtctcgcgcg catcctcgaa gcgggcggtt gcggtgttgg acagatagtg caggccgcga
      481 tgcacgttgg cgtattccat cttgtaggca tgctcgatcg cttccagcac cggccgcggt
      541 ttctgggccg aggccccgtt gtcgagatag acgagcggct tgccgtaaac ctcacggaac
      601 agaatgggaa aatccgtgcg gatggtgtcg acgtcgtagt tggcgccgcc cttgagctcg
      661 gcccccttga tcatcgcggt catgtgcctt cctccgccgt tgccaacggc gtcatctcac
      721 gtgctcaatt gcgccaagcg gcgtcgggcc gcggccatga gcgccgggcg cagctctgcc
      781 tcaaccttgt cgatcgcctc gccgatgaag ctctcaatca gcagggcgcg cgcttcctct
      841 ttgccaatgc cgcgggcgcg gaaatagaac agcaggtcct catcgatctc cgccgaggtc
      901 gagccgtgcc cacacaccac gtcgtcggcg ttaatctcga gctcgggctt ggaatcgaac
      961 tcggcgtcct cggacagcat caggacctgc gccatttgct tgccgtcggt cttctgcgca
     1021 tcgggccgca cgatcacttt gccctggaac acaccccggg ccctgtcggc caatacgccc
     1081 ttgaacagtt cgcggctttc gcagccagcc tgcgcatggt cgatcaccag cgtgctgtcg
     1141 atgtgttcgt tcgcccggcc cagaaaacag ccggagacat cggccttggc tccctcgccg
     1201 gtaaaggtca ggtcgaggcc gtggcgcacc aggccggtgc cggcggtgaa ctggaaggcg
     1261 cgatagctcg caccggcgcc aagccgcggc cgccaggtcg ccagatggct ctcggcacca
     1321 cccgcaagcg tcagtttgac gtgcgccagc tcggcgccct cggcaatggc caattcgctc
     1381 aaggcgttgc tctggccggc ggcggcgccc ggcaggctca gatgcgcctc gatcaccgtg
     1441 gcgctcgcct cggcgccgac gctgaggagg ttgcgcgtgg tgatgaggcg ggcctcgccg
     1501 gcgctgcgga taaaaagcaa gagcagcggc ttggcgagtt tggcaccccg ggcgatgtcg
     1561 atcacggcgc catcgctcat aaaggcggtg ttgagcgcca ccacggcatc ctgttcggcg
     1621 gccgccgcca ggccgtcagc agccacgccc tcgggcttag ccaaccaggc cgccagtggc
     1681 gcgaagctga gcccagccag cgccccctgt gcggagagcg cctgggcata aacgccgtcg
     1741 acgaacacca ggcgatggac gtcgagctcg gcgaatgcct cgagcgcggt gtcgatctgc
     1801 gcggccgttg gcaaggcacg cccggcgcca acggcggcgg gcagcgcctc tttcagggcg
     1861 gagcgcagat cggtgtactt ccaggcctcg agccgccgat gcggcaggcc cagcgccgca
     1921 aaggcgccga tcgctgcccg gcgcgccgct gccacggcgg ggccgcccgg cagctgggtc
     1981 gcggccgcct cgaagttttg ggtgaagccc tgctcggcct tcgtccttgt tatggccact
     2041 gtcatctcac gttcactttt tctggaccgc cgcggcgtgc ttttttctgc agcgcggcgg
     2101 cgcacactgt cgccgctcag gccgctcggc tttggaattc ggcgtagccc gtcttttcca
     2161 gctcgagcgc cagctccttg cccccggatt tctggatctt gccggcggcc atcacgtgca
     2221 ctcggtcggg cacgatgtag ttcagca
//
LOCUS       DASTMT010000506         4888 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_1273339, whole genome shotgun sequence.
ACCESSION   DASTMT010000506 DASTMT010000000
VERSION     DASTMT010000506.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4888)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4888)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4888
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1273339"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..611)
                     /locus_tag="VFR00_11635"
     CDS             complement(<1..611)
                     /locus_tag="VFR00_11635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEU0159963.1"
                     /translation="MSGVPDGLTPLLLARLAEEEKAGTSVRLLHVARDDRRLEALAEG
                     IGFFAPKLRVIKMPAWDTVPYDRIGPNAEIVASRMAALARLSAAAQKGPAVLITTVNA
                     VLQRLPPRDFIRRSLKNIAPGQRLDMQELIERLNLAGFQRTGTVMEPGEYAVRGGILD
                     VYPPGRSSPVRLDFFGDTLEHIKAFDPDTQRTSKVVQRVSLLP"
     gene            complement(699..965)
                     /locus_tag="VFR00_11640"
     CDS             complement(699..965)
                     /locus_tag="VFR00_11640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase assembly factor 2"
                     /protein_id="HEU0159964.1"
                     /translation="MPEDVDTRRRRAAYRAWHRGTKEMDLMLGRFADSALPGMSPETL
                     ARFERLLALPDPDLQDMLLNPALRAGPEALADLIARVRAFHGLD"
     gene            1149..3266
                     /gene="recG"
                     /locus_tag="VFR00_11645"
     CDS             1149..3266
                     /gene="recG"
                     /locus_tag="VFR00_11645"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEU0159965.1"
                     /translation="MRPLELTPLFASATSLTGVGPRVALLLKKALRLPAGTLQARSID
                     LIWHTPTGIIDRRATPTVAAALPGTIATLEVRVLKHKPGPRGNTRAPYKVVCEDDSGR
                     IELVFFHAERKFIERQLPVGSLRYVSGRVESYNDGKQMVHPDYVLPPEARADLPMLEP
                     VYPLTAGLSGKILLKACRQALDRVAALPEWQEAAWLKQRGWPPFRRALELLHRPEDAS
                     DVSPAAAPWQRLAYDELLAGQLALALVRQSLKNQPGRSVEGDGAIRARIVAALPFALT
                     NAQRQALKEIAEDSRAPFRMLRLLQGDVGSGKTVVALLAMAIAVEAGAQAALMAPTEV
                     LARQHADTIAPLADAAGLRLGLLTGREKGGARAELLARLGRGEIDILIGTHALFQADV
                     AFKDLAFAVIDEQHRFGVHQRLALQAKGGKGGTNVLVMTATPIPRTLLMTHYGDLDVS
                     RLTEKPKGRKPILTKSVPVEALERLLARLKVQIREGAQVYWVCPLIEGSDRSDLAAAE
                     ERHAHLVQIFGAAAVGLLHGAMPDRAKDATMAAFAGNRHQILVATTVIEVGVDVPNAN
                     IMVIEHAERFGLAQLHQLRGRVGRGSRESFCLLLHKAPLGDTARRRLEMMEATEDGFQ
                     IAEKDLELRGGGELMGARQSGLPEFRVAQVANFPELLAAARDDARLVLSQDPMLVSGR
                     GQALKLLLYLFECDEAVRLFRAA"
     gene            complement(3282..4109)
                     /locus_tag="VFR00_11650"
     CDS             complement(3282..4109)
                     /locus_tag="VFR00_11650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HEU0159966.1"
                     /translation="MHDNPASSASRSAPDETHDLKAELAKLAQDETAEIKAGLAKLAL
                     NPVPEMHQRTGARLRNYFLTGLVVVGPVTITLYIAWYFINIIDAWVKPYIPKIYNPES
                     YVPFPIPGVGLVFAIIGLTMIGALTANLIGRSLISAGEMMLGRTPIVRNVYQGLKQIF
                     ESVVTASAPNQSFQKVALMEFPSKGIWSIVFITGDAAEQIAAEVPGQDLISVFMPTGM
                     LPPSGFVCFVPRKDVMPVKMTVEDAAKIIISAGMVNPETQSKLQDLAQRARNGPKAA"
     gene            complement(4292..>4888)
                     /locus_tag="VFR00_11655"
     CDS             complement(4292..>4888)
                     /locus_tag="VFR00_11655"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HEU0159967.1"
                     /translation="GGWDLFANLFGGGTAASPVAVQPAAAAAPRPEAPTAAAPRPDPG
                     SAAPRPDTAAILNAENSTAERSAPARRTQANGWSRQTQVESERAARVETAAITTKEEP
                     QRRATVVPEPQARSGNLRIQIAAVRTQAEAQALAAKVMREHASTLAKREPQIDQTIMG
                     NMGSFYRVLIGPFASAQETQSICAKLKGTGLDCLVTAQ"
BASE COUNT          773 a         1625 c         1682 g          808 t
ORIGIN      
        1 atcggcagca ggcttacgcg ctgcacgacc ttgctggtgc gctgggtgtc gggatcgaac
       61 gccttgatgt gctccagcgt gtcgccgaaa aaatcgagcc gcaccggact ggagcgcccg
      121 ggcggataaa cgtcgaggat accgccgcgc acggcgtact cgccgggctc catgacggtg
      181 ccggtgcgct ggaagcccgc gagattgagg cgctcgatca gctcctgcat gtcgaggcgc
      241 tggccaggcg cgatattctt gagcgaacgg cgaatgaagt cgcgcggcgg caagcgctgc
      301 agcacggcat tgaccgtggt gatgagcacc gccggcccct tctgcgcggc cgcgctcagg
      361 cgggccagcg ccgccatgcg gctcgccacg atctccgcat tgggaccgat gcgatcgtag
      421 ggaacggtat cccaggccgg cattttgatg acgcgcagct tgggtgcaaa aaatccgatg
      481 ccctcggcga gcgcctcgag gcggcgatcg tcgcgcgcca cgtgcaggag gcgcacggag
      541 gtgccggctt tttcctcctc cgccaggcgc gccaacagca agggggtaag gccatccggc
      601 acgccggaca tgagctcgca cccgccctcg gcaggcgtgc ctccccgggc cgcgtcttgc
      661 cccgtcgtct gcttgtcctg gccggggcta cgcaaggctc agtccaatcc gtgaaacgcc
      721 cgcacccggg cgatcaggtc cgccaaggcc tcgggtccag ccctcagcgc ggggttgagc
      781 aacatgtcct gcaggtcggg atcgggcagc gccaggaggc gctcgaagcg cgccagggtc
      841 tcgggcgaca tgccggggag cgcgctgtcg gcgaagcgcc ccagcatcaa gtccatctcc
      901 ttggtgccgc ggtgccacgc ccggtaggcg gcgcggcggc ggcgggtgtc gacatcctcg
      961 ggcatgggct taattcggct cggataactc tggctcggcg cttgacggcg cccctcgagc
     1021 gcggccaccc tcaatgagcc ctttcggcgc tgtcatatag tgcggcattg cgcagggcgc
     1081 cagcctgcca aaagggcatc gtggcggcgc ttgcccgctt ctgtcgtgga gcccatagaa
     1141 atcctgcgat gcggcccctt gagctgaccc ccctcttcgc ctccgccacg agcttgaccg
     1201 gcgtcggccc gcgcgtggcc ttgctgctca agaaggccct gcgcctgccg gccggcacgc
     1261 tgcaggcgcg cagcatcgac ctcatctggc ataccccgac cgggatcatc gaccggcgcg
     1321 cgacccccac cgttgccgcg gcgttgccgg ggacgattgc caccctggaa gtgcgcgtgc
     1381 tcaagcacaa gcccggaccc cgcggcaaca cgcgcgcacc ctataaggtg gtgtgcgaag
     1441 acgatagcgg ccgcatcgag ctcgtcttct tccacgcgga acgcaaattc atcgagcgtc
     1501 agctccccgt cggcagcctt cgctacgtca gcgggcgcgt ggaaagctat aacgacggca
     1561 agcagatggt gcatcccgat tacgtcctgc cgccggaggc gcgggccgat ctgccgatgc
     1621 tcgagccggt ctatcccttg acggcaggcc tttcgggcaa aatcctgctc aaggcttgcc
     1681 ggcaggcgct cgatcgcgtg gccgcgctgc cggaatggca ggaggcggct tggctcaagc
     1741 agcgcggctg gccgcccttt cgcagggcgc tcgagctgct gcaccgtccg gaagacgcaa
     1801 gcgacgtctc ccccgccgcc gcgccctggc agcggctcgc ctacgacgag ttgttggccg
     1861 gccagctggc gctggcgctc gtgcgccaaa gcctgaagaa ccagcccggc cgcagcgtcg
     1921 agggcgacgg ggccatccgc gcccggatcg tcgccgcgct gcccttcgcg ctgaccaacg
     1981 cgcagcgcca ggcgctgaaa gaaatcgccg aggacagccg cgccccgttc cgcatgctgc
     2041 gcctgttgca gggcgatgtg ggctcgggca agacggtggt ggcgctgttg gccatggcca
     2101 ttgcggtgga agccggcgcg caggccgcgc tgatggcgcc cacggaggtg ctggcccgcc
     2161 agcacgccga caccatcgcg ccgctggcgg atgccgcggg tctgcgcttg ggcttgctga
     2221 ccggccgcga aaagggtggc gcccgcgccg agctactcgc ccgcctgggc cgcggcgaga
     2281 tcgacatcct gatcggcacg catgcgctgt tccaggcgga tgtggccttc aaggacctgg
     2341 ccttcgccgt gatcgacgag cagcaccgct ttggcgtgca tcaacgcctg gcgttgcaag
     2401 ccaagggcgg caagggcggc accaacgtct tggtgatgac ggcaacgccg atcccgcgca
     2461 cgctgctgat gacgcactac ggcgatctcg acgtttcgcg gctgacggag aagccgaagg
     2521 gccgcaaacc cattttgacc aaatcggtgc ccgtggaggc gctggagcgc ctgctcgcac
     2581 ggctcaaggt gcagatcagg gagggcgccc aggtctactg ggtgtgcccg ttgatcgaag
     2641 gcagcgaccg ctcggatttg gctgccgccg aagagcgcca tgcgcatctc gtgcagatct
     2701 tcggggcagc ggccgtgggc ctcttgcacg gtgccatgcc ggatcgggcc aaggatgcga
     2761 ccatggccgc ctttgccggc aaccggcacc agatcctggt cgccaccacg gtgatcgagg
     2821 tcggcgtcga cgtgccgaac gccaacatca tggtgatcga gcacgccgag cgtttcggcc
     2881 tcgcgcaact gcatcagctg cgaggccgcg tcgggcgagg cagccgcgaa agcttctgcc
     2941 tcttgctgca caaagccccg ctcggcgaca ccgcgcggcg gcgcctggag atgatggagg
     3001 cgaccgagga cggctttcag atcgccgaga aggacctgga gctcaggggc ggtggcgagc
     3061 tgatgggcgc ccggcagagc ggcctgcccg agttccgtgt cgcgcaggtt gcgaactttc
     3121 ccgagctgtt ggcggcagcg cgcgacgacg cccgcctcgt actgagccag gacccgatgc
     3181 tcgtctccgg gcgcggacag gccctcaagc tcttgcttta tctgttcgag tgcgacgagg
     3241 ccgtgcgcct gttccgtgcg gcttgaagac ggcgcgccgc ctcaggccgc tttcggcccg
     3301 ttgcgcgccc gctgcgccag gtcctgcagc ttcgattggg tttccgggtt caccatcccc
     3361 gccgagatga tgatcttggc cgcgtcctcg acggtcatct tgacgggcat gacgtccttg
     3421 cgcggtacga agcagacgaa gcccgacggc ggcaacatgc cggtcggcat gaacacgctg
     3481 atcaagtcct ggccgggcac ctcggcggcg atctgctcgg cggcatcgcc ggtgatgaac
     3541 acgatcgacc agatgccctt ggaggggaat tccatcagcg ccaccttttg gaagctctgg
     3601 ttgggcgcgg acgcggtcac gaccgattcg aagatctgtt tcaggccctg gtaaacgttg
     3661 cgcacgatgg gcgtgcgccc cagcatcatt tcgccggccg agatgagcga gcgaccgatc
     3721 aggttggccg tgagagctcc gatcatggtg aggccgatga tggcgaacac caggccgacg
     3781 cccgggatcg ggaagggaac gtaggattcg gggttgtaga ttttcgggat gtagggcttc
     3841 acccaggcat cgatgatgtt gataaagtac caggcgatgt aaagcgtaat ggtcaccggc
     3901 ccgaccacga ccagcccggt caggaagtag ttgcgcaggc gcgccccggt gcgctggtgc
     3961 atctccggga cgggattgag cgccaactta gcgagaccgg ctttgatttc agccgtctcg
     4021 tcctgcgcca gcttggccag ctccgccttg aggtcgtgtg tctcgtccgg cgccgaacgg
     4081 gacgcggacg aggccggatt gtcatgcatc gggacggccc acaatcgagc tggcacgcaa
     4141 gagcagaaac ggccagactc aggtatccgg cccattgtca catgagacgt tcccgaggca
     4201 aggccggcga acacccccag ctctcaggtg atctcggcgc tcggcgctag tctattggaa
     4261 tgaaataagt ttttcaaatc tggcgcgggg cctattgcgc cgtcaccagg caatcgaggc
     4321 ctgtgccttt gagcttggca caaatgcttt gcgtttcttg cgcgctggca aacgggccga
     4381 tcagcacgcg atagaacgag cccatgttgc ccatgatggt ttgatcgatt tgcggctccc
     4441 gcttggcaag cgtgctcgcg tgctcgcgca tcaccttggc ggcgagcgcc tgggcttccg
     4501 cctgggtgcg caccgcggcg atctgaatgc gcagattgcc gctcctggcc tgtggttcgg
     4561 gcacgaccgt ggcgcgacgc tgcggctcct ccttggtggt gatggcggcg gtctccacgc
     4621 gcgcggcgcg ctcgctctca acttgcgttt gccgcgacca accgttggct tgggtgcgcc
     4681 gggccggcgc cgagcgctcg gcggtcgaat tctcggcatt caagatcgcc gccgtgtcgg
     4741 ggcggggcgc ggcgctcccc gggtcagggc ggggcgcggc ggctgtcggc gcttcagggc
     4801 gcggcgcggc ggcggccgcc ggctgcaccg ccacggggct cgcagccgta cccccaccaa
     4861 acaggttggc gaagagatcc caaccgcc
//
LOCUS       DASTMT010000507         1541 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_6053489, whole genome shotgun sequence.
ACCESSION   DASTMT010000507 DASTMT010000000
VERSION     DASTMT010000507.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 1541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1541
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6053489"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..201)
                     /locus_tag="VFR00_11660"
     CDS             complement(<1..201)
                     /locus_tag="VFR00_11660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0159968.1"
                     /translation="MSTLALPAAAALAATPPARRQGAQRLLAARLRRAETAPAAAGPT
                     ADADLNLKRPRKETPPCPQSSPA"
     gene            330..1217
                     /locus_tag="VFR00_11665"
     CDS             330..1217
                     /locus_tag="VFR00_11665"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEU0159969.1"
                     /translation="MRGLNPDHLRTLSEVVAQASFTRAARRLNLAQPTVSLQVRELES
                     RLGVRLVDRLGKRAFATAAGRELIEHARRLAQEEERLLAAMRRHREGWLGQVRIGSST
                     TALIYHLPPVLQQLRTAQPNIELVVTTGTTNAIVERIIRNDIDLGVVSLPVNDRLLQV
                     EPLLDEPLVAIFSANVRGLPARVTPSYLLKHPLLLEFARAHVRAMIIEWLSTQEGEAH
                     PAMELDNLEAVKRMVAAGLGSSIVPIAAVSKENARNDLVARPLRPALTRTLALIQRRD
                     RADDGALQQVRTALFTLKG"
     gene            complement(1256..>1541)
                     /locus_tag="VFR00_11670"
     CDS             complement(1256..>1541)
                     /locus_tag="VFR00_11670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018183377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEU0159970.1"
                     /translation="FEGDVPVTNNEAAKTQFVVGVAAAIVGADNVKPMRPIMGAEDFS
                     HMLNKRPGAFIMIGNGIGPGLHNPAYNFNDEAIPIGVSYWARLAETAMPI"
BASE COUNT          248 a          503 c          502 g          288 t
ORIGIN      
        1 agccggagag gactgtggac atggcggtgt ctcctttcga ggtcttttta agttgaggtc
       61 ggcgtcggcc gttggcccgg cagcggctgg tgcggtctcc gcccgccgca ggcgcgccgc
      121 aagcagccgc tgggcgccct ggcgccgcgc cgggggcgtt gccgccaagg cggccgcggc
      181 cggcaaggct aacgtgctca tgatcgtctc cttttttggc gccgggcgcc tcggcatggc
      241 cgcggcttaa gcgctgctgc tcagatgggg tggactcttg aattgttcaa atttattatt
      301 ccaatgaata tgattggaaa ctctaataga tgcgtggtct caatcccgat cacctgcgca
      361 cgctgagcga ggtggtggcg caagcgagct tcacgcgcgc cgcgcggcgc ctgaacctgg
      421 cgcagccgac cgtctccttg caggtgcgcg agctcgaatc gcggctcggt gtgcgcctcg
      481 tcgatcgtct gggcaagcgt gccttcgcca ccgccgccgg tcgagagctg atcgagcacg
      541 cgcgtcgcct tgcccaggag gaagagcggc tgctggccgc catgcgccgc catcgcgagg
      601 gctggcttgg tcaagtgcgc atcggttcca gtaccacggc gttgatctac cacctgccgc
      661 ccgtgctgca gcagttgcgc acagcccaac ccaacatcga gctggtggtg acgaccggca
      721 ccaccaacgc catcgtcgag cgcatcatcc gcaatgacat cgatttgggc gtcgtcagcc
      781 tgccggtgaa cgatcgcttg ctacaggtcg agccgctgct cgacgaaccc ttagtggcaa
      841 tcttctcggc caatgtccgc ggtttgccgg cgcgcgttac gccgtcctat ctgctcaaac
      901 atccgctcct gcttgaattt gcccgcgcgc acgtccgcgc catgatcatc gagtggcttt
      961 cgacgcaaga gggcgaagcc caccccgcca tggagctcga caacctcgag gccgtcaagc
     1021 gcatggtggc ggcgggtctc ggtagttcga tcgtgccgat cgccgccgtc tccaaggaaa
     1081 atgcccgcaa tgacctggtg gcgcgtccgc tgcgaccggc cttgacgcgc acgcttgcgc
     1141 tcattcagcg gcgcgaccgg gccgacgatg gcgccctaca gcaggtgcgc acggccttgt
     1201 tcaccctcaa gggctaacga ggggagcgag cacccgttcg gcaggcagag gtcggtcaga
     1261 tcggcatggc cgtctcggcc agccgcgccc aataggagac gccgatcggg atggcctcgt
     1321 cgttgaaatt gtaggcggga ttgtgcaggc ccgggccaat gccgttgccg atcatgatga
     1381 aggcgcccgg acgcttattg agcatgtgcg agaaatcctc cgcccccata atcgggcgca
     1441 ttggcttgac gttgtcggcg ccgacgatcg cggcggcgac gccaacgacg aactgcgtct
     1501 ttgccgcctc gttgttggtg accggcacgt cgccctcgaa c
//
LOCUS       DASTMT010000508         9727 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_947154, whole genome shotgun sequence.
ACCESSION   DASTMT010000508 DASTMT010000000
VERSION     DASTMT010000508.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 9727)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9727)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9727
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_947154"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..628)
                     /locus_tag="VFR00_11675"
     CDS             complement(<1..628)
                     /locus_tag="VFR00_11675"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEU0159971.1"
                     /translation="MAIRGILFDKDGTLIDYWLTWLPINHQVALFAAGGDEALARRLL
                     LAGGQDPASDRVEPGSVLAAGSVDDIARVFAAELGTTSPARLAPGIEAIFRRGGAEHG
                     RLVPGARDTVIELRRRGFRLGLATNDSIGGLEASLARHGIVDLFDFTAGCDSGFGAKP
                     DPRMALAFCASLGVPPSAVAVIGDAPHDLCMGRAAGVGLTLGVLSGTSA"
     gene            complement(649..1482)
                     /locus_tag="VFR00_11680"
     CDS             complement(649..1482)
                     /locus_tag="VFR00_11680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013950347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HesA/MoeB/ThiF family protein"
                     /protein_id="HEU0159972.1"
                     /translation="MTLGPEEIERYKRHLVLHEVGGQGQQRIKAAKVLIVGAGGLGSP
                     ALMYLAAAGVGTLAVIDDDDVSLDNLQRQILHDTPHVGSSKVASAKAALGRLNPHVVV
                     EAHAERLTANNALDIISRYDIVADGSDNFATRYLVSDACYLAKKTLVFAAVGPFDGYL
                     STLKPHERGPDGEPFPSYRCMFPEAPPPGTVANCAEVGVLGSIVGVIGALQATEVLKE
                     IVGVGQSLAGRLLIYDARATRFTEIKVTWDPANPLSGKEPSIRDLSAHARAGNDPACA
                     A"
     gene            complement(1479..2417)
                     /locus_tag="VFR00_11685"
     CDS             complement(1479..2417)
                     /locus_tag="VFR00_11685"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEU0159973.1"
                     /translation="MKIVVMGAGGVGGYFGARLAAAHHDVTFVARGAHLEAMRRAGLR
                     LDSDIGALAQPVKVAAEAHEIAAAECIIFAVKLGDTEAAADQLRGLVAGGATVFTFQN
                     GVESAERIGYRLGPENVVPGVARISAQISAPGVITQRGKFASLEFGEPDRAPRPRAGA
                     LLEACKGAGISAAISPDIARALWLKFAMLAPMSGLTALTRGPIGPVRADPQARQLLEA
                     AVREVVALGMAAGVRLEPADADAVITAIDALHPGIMASMCHDLLAQKKLEIAGLSGAV
                     VRLGRARGVAVPTHTFITQALSLFADGKPKLQGSHA"
     gene            complement(2441..3487)
                     /locus_tag="VFR00_11690"
     CDS             complement(2441..3487)
                     /locus_tag="VFR00_11690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006613428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HEU0159974.1"
                     /translation="MLRQSLVEYAKPLAPTEAPTPTVSGREVLLRVSHCGVCHSDLHL
                     QDGYFDLGGGQKLDVRGNRPLPFTLGHEIAGSVEAAGADAGVAPGQRYAAYPWIGCGK
                     CALCARGDEHLCNAPRVLGVTVDGGYATHVIVPDPRYLLDVTGIAPELAGALMCSGLT
                     GYSAIKKASPYLRAGPLLIVGLGGVGMMGLQFARAITDGPILVADIDPSKRAAAVELG
                     ASEAFDPADAGARRAIIKASGGGAGAAVDFAGSDKSLNFAQGAVAKGGAVVIVGLIGG
                     SFSLPVPMFPLRALTIMGSYVGSLAEAREMLALVQAGKVAPIPVELRQFEETSAALDD
                     LRAGRVVGRVVITP"
     gene            3649..4296
                     /locus_tag="VFR00_11695"
     CDS             3649..4296
                     /locus_tag="VFR00_11695"
                     /inference="COORDINATES: protein motif:HMM:NF013933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HEU0159975.1"
                     /translation="MGLDQEVLWAFTLLWLAILPTPGANSLLIIQLALSSGWRSVAIA
                     LSGNLFGIACYALATLLGLAWLLAAAPSVRLAIYGLGGLYLAWTGIRLAARGLKRGQA
                     PDAAPSQAVAASAAGTFARGLLTALANVQALFFLASIFASVGVLSAPLATRLAAIAVI
                     VIGNGAYLTLLAWLMQKPAARTFYHRYQPRMEVAFGALFLLFGARLLAREWAGWL"
     gene            complement(4322..4717)
                     /locus_tag="VFR00_11700"
     CDS             complement(4322..4717)
                     /locus_tag="VFR00_11700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0159976.1"
                     /translation="MRLATRAIATGALICLAIAPTAAEARWERLGCQKVGFITDKDII
                     RVGRGEGRFKSIRLQVSGNKVYMDDLKVIYANGEPDDIPVRSEIRAGGQTRPLDLRGE
                     RRAIKQIEMKYRSQPNFKGEATVCVDAQS"
     gene            complement(5248..7455)
                     /gene="pnp"
                     /locus_tag="VFR00_11705"
     CDS             complement(5248..7455)
                     /gene="pnp"
                     /locus_tag="VFR00_11705"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013950357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEU0159977.1"
                     /translation="MFDTQRVEIDWAGRKLVLETGRMARQADATVLAQYGETSVLATV
                     VAEKTERPGIDFFPLTVNYQEKTFAAGKIPGGYFKREGRPTEKETLTSRLIDRPIRPL
                     FVEGFKHETQVVATVLSHDMENDPDIVALVAASAALTLSGVPFLGPIGGARVGVIDGK
                     LVLNPMIDEMAKSDLDLVVAGTGDAVMMVESEAKELPEQLMLEAVMHGHSGFQPVIQA
                     IIRLAEKAAKEPWDFKAADKSKYKSKAKALIEADLRQAFATPEKQKRHELVEAANAKL
                     KAELLGPEADGGEEVLLGAVFKELEQEVVRGDIIRTQRRIDGRDLTTVRPIRSEVHVL
                     PRTHGSALFTRGETQALVVATLGTGEDEQYVDALEGTRKERFLLHYNFPPYSVGETGR
                     MGSPGRREIGHGKLAWRAVHPLMPSAEEFPYVVRIVSEITESNGSSSMATVCGSSLAL
                     MDAGVPLKRPVAGIAMGLIKEGNDYAVLSDILGDEDHLGDMDFKVAGTEKGVTSLQMD
                     IKIAGITEEIMRVALEQAHHGRMHILGEMSKALGSARTELGEHAPRIETIKIPVDKIR
                     EVIGSGGSVIREIVAESGAKIDIEDDGTIKIAAAKREAIEAALARIRGITSEPEVGQV
                     YKGKVVKIMEFGAFVNFFGAKDGLVHISQLTNGRPETVGEVVKEGQEVYVKLLGFDDR
                     GKVRLSMKVVDQATGKEIPRAEGEAEERFDGGGRPPRRERGDRDRGERRRERG"
     gene            complement(7576..7917)
                     /gene="rpsO"
                     /locus_tag="VFR00_11710"
     CDS             complement(7576..7917)
                     /gene="rpsO"
                     /locus_tag="VFR00_11710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018062720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEU0159978.1"
                     /translation="MRPGWTTSRPRPPRSLQHTRKVKPMSLSSQRKLQLVKEYATKKN
                     DTGSPEVQIALLTERINGLTEHFKLHKNDNHSRRGLLKMVSLRRRLLDHVKSRDEGRY
                     QKIIERLGIRR"
     gene            complement(8012..8944)
                     /gene="truB"
                     /locus_tag="VFR00_11715"
     CDS             complement(8012..8944)
                     /gene="truB"
                     /locus_tag="VFR00_11715"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEU0159979.1"
                     /translation="MRRASGKKGKAVNGWLVLDKPAGVTSTYAVGAMRRAFGARKAGH
                     AGTLDPAATGVLPIAFGEATKTVPYAVDGEKCYRFTVRWGAQTETDDAEGRVLATSTQ
                     RSDAAAISRLLAQFTGEILQTPPAYSAIKVAGRRAYDLARGGEAVSLEPRPVLIHSLR
                     LIDTPDADSAVFEARCGKGTYVRALARDMGRVLGCLGHLIALRRTRVAGFEEAAAISL
                     NSVELALEHGEAALLRLLLPIEAALNDFAVLNVGQAEAARLLNGQPILLRGRDAPYQP
                     GPTYALCRGHLVALGEIARGELHPIRVFHLGDAG"
     gene            complement(8941..9339)
                     /gene="rbfA"
                     /locus_tag="VFR00_11720"
     CDS             complement(8941..9339)
                     /gene="rbfA"
                     /locus_tag="VFR00_11720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEU0159980.1"
                     /translation="MGRRHSGKSTGPSQRQLRVGELVRHALAELLQRGEIHDEVLAAH
                     ALTIPEVRMSPDLRLATIFVMPLGGKDIEAVLAALDSHRRYIRGEVARAVNLKFAPEV
                     RFRADTTFDEALRIDRLLASDKVRQDLDKQ"
     gene            complement(9410..>9727)
                     /locus_tag="VFR00_11725"
     CDS             complement(9410..>9727)
                     /locus_tag="VFR00_11725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009734865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HEU0159981.1"
                     /translation="LLAPTLREIFLGNAQILEVFNISKVGKVAGCRVTEGKVERGAKV
                     RLIRDNVVIHEGTLSTLKRFKDEVREVPAGQECGMAFANYQDIRAGDVIECFNVETIA
                     RTL"
BASE COUNT         1560 a         3351 c         3154 g         1662 t
ORIGIN      
        1 gcgcgctggt gccgctcaac acgccgagcg tcagccccac gcccgccgcg cgccccatgc
       61 acagatcatg cggtgcgtcc ccgatcacgg ccacggcgct cggcggcacg ccgaggctgg
      121 cgcaaaacgc caaggccatg cgcgggtcgg gtttggcgcc gaagccggaa tcgcacccgg
      181 cggtgaagtc gaacagatcg acgatgccat gccgggccag cgaggcctcg aggcccccga
      241 tcgaatcgtt ggtggccagc ccgagacgga acccccgccg gcgcaattcg atcaccgtgt
      301 cccgggcccc tggcacaagg cggccgtgct cggcgccgcc gcggcgaaag atcgcctcga
      361 tgcccggcgc cagccgggcg gggctcgtgg tccccagctc ggcggcaaag acgcgggcaa
      421 tgtcgtcgac gctgccggcc gccagcaccg atcccggttc gacccggtca ctcgccggat
      481 cctgcccgcc cgccagcagc agcctgcggg caagggcctc atcgccgccg gcggcgaaca
      541 gggcgacctg gtggttgatc ggcagccagg tcagccaata atcgatcagg gtaccatcct
      601 tatcgaacag gatgccgcgg atcgccatgt tgcctccggc gcacgtcgtc aggccgcgca
      661 agccggatcg ttgccggccc gggcgtgcgc ggagagatcg cggatgctgg gttctttgcc
      721 ggatagcggg ttggccgggt cccacgtcac cttgatctcg gtaaagcgcg tggcgcgcgc
      781 atcgtagatc aacaggcgtc ccgccaggct ttggccgacg ccgacgatct ccttcagcac
      841 ttcggtggcc tgcagggcgc cgatcacacc gacgatcgag ccaagcaccc ccacctccgc
      901 acaattggcc accgtacctg ggggcggcgc ctccggaaac atgcagcggt agctcgggaa
      961 cggttcgccg tcgggccccc gctcgtgcgg tttcaaagtg ctgagataac cgtcgaaggg
     1021 gccgacggcg gcaaagacca gcgtcttctt ggcgagataa caggcatcgc tgacgagata
     1081 gcgcgtggca aagttatcgg agccgtcggc aacaatgtcg tagcgcgaga tgatgtcgag
     1141 cgcattgttg gccgtgaggc gctccgcgtg ggcctcgaca acgacgtgcg gattgagccg
     1201 gccaagcgcg gctttggcgc tcgccacctt ggagctgccg acatgcgggg tgtcgtgcag
     1261 gatctggcgc tgcaagttgt cgagtgagac gtcatcgtcg tcgatgaccg caagcgtacc
     1321 gacgccggcc gcggccagat acatgagggc gggagagcca agcccgccgg ccccgacgat
     1381 gagcaccttg gcggctttga tgcgctgctg gccctggccg ccgacctcgt gcaacaccaa
     1441 gtggcgctta tagcgctcga tctcttccgg gccgagtgtc atgcgtgcga tccctgaagc
     1501 ttgggtttgc cgtcggcgaa cagcgacagc gcctgggtga taaaggtatg cgtcggcacc
     1561 gcgacgccgc gcgcccgccc cagccgcacg accgcgccgg acaagccggc gatctccagc
     1621 ttcttttgcg ccaacaggtc gtgacacatg gaggccatta tccccggatg cagggcgtcg
     1681 atggccgtga tcacggcgtc cgcatcggcg ggttcgaggc gcacccccgc cgccatgccg
     1741 agcgccacca cctcgcgcac ggccgcctcc agcagctggc gggcctgagg gtcggcccgc
     1801 accggcccga tcggcccgcg cgtcagcgcg gtgagccccg acatcggtgc cagcatggcg
     1861 aacttcagcc acagcgcgcg ggcaatatcg gggctgatcg ccgcgctgat gcccgcccct
     1921 ttgcaggcct cgagcaacgc ccccgcgcgc gggcgcgggg ctcgatcggg ctcgccgaac
     1981 tcgaggcttg cgaacttgcc gcgttgcgta atgacgcccg gcgccgaaat ctgcgcactg
     2041 atgcgtgcca ctcctggcac gacgttctcg gggccgagcc ggtaaccgat gcgctcggcg
     2101 ctttcaacgc cgttctgaaa ggtgaagacc gtcgcgcccc cggcgacgag cccccgcaac
     2161 tggtcggcgg ctgcttccgt gtcgccgagc ttgacggcaa aaatgatgca ctcggccgcg
     2221 gcgatctcgt gcgcttcggc tgcgaccttg accggctggg ccagcgcgcc aatgtcgctg
     2281 tcgagccgca gtcctgcccg gcgcatggcc tcgaggtggg cgccgcgcgc cacgaaggtg
     2341 acgtcgtgat gggcagcggc caggcgcgcc ccgaagtagc cgccaacgcc acccgccccc
     2401 atcactacta ttttcatcga gctcatccgc tgcggcgcgc tcaaggggta atgacgacgc
     2461 ggcccaccac ccggcccgcc cgcaaatcat cgagcgccgc gctcgtctcc tcgaactgcc
     2521 gtagttcgac gggaatcggc gccactttcc ccgcttgcac caaagctagc atttcgcgcg
     2581 cctcggcaag cgaaccgacg tagcttccca tgatggtgag ggcccgcagc gggaacatcg
     2641 gcacaggcag ggagaagctg ccgccgatca ggccgacgat gacgacggcg ccgcccttgg
     2701 ccaccgcgcc ctgggcaaag ttgagcgact tgtccgaacc cgcaaaatcg acggcagcgc
     2761 cggcgccgcc cccggacgcc ttgatgatgg ccctgcgtgc gccggcatcg gccgggtcga
     2821 aggcctcgct ggcgcccagc tcaacggcgg ccgcgcgctt gctgggatcg atatcggcca
     2881 ccaagatggg cccatcggtg atggcccggg cgaactgcag gcccatcatg ccgacgccgc
     2941 cgagcccaac gatcaagagc gggccggccc tgaggtaggg gctcgccttc ttgatggcgc
     3001 tgtagccggt caggccggag cacatcagcg caccggcgag ctcaggggct atccccgtca
     3061 cgtccagcaa gtagcgcgga tcgggtacga tcacgtgtgt ggcatagcca ccgtcaaccg
     3121 taacgccgag cacgcgcggg gcattgcaca gatgctcatc gccgcgcgcg caaagtgcgc
     3181 acttgccgca accgatccag gggtaggcgg catagcgctg gcccggcgcc accccggcgt
     3241 cggcgcccgc agcctcgacg ctgccggcga tctcgtggcc tagcgtgaac ggcagcggcc
     3301 gattgccgcg cacatcgagc ttctggccac caccaagatc gaaatagccg tcctgcagat
     3361 gcagatcgga gtgacagacg ccgcaatggc tcacgcgcag cagcacctcg cgacccgaca
     3421 ccgtgggggt gggcgcctcg gtgggcgcga gcggcttggc atattcgacg agggattgac
     3481 gcagcattaa ggaccgtcct tgttgggatc gcccaagtct agcagaaggg agcgggcgcc
     3541 gacgagccct cgaggcgagc gccggcggcc gcgagaggcc cggggacctc gggccagagg
     3601 ccgggctcgg cgctgccctc ggtcagcaca ttggcaagga ggcggccctt ggggctcgac
     3661 caggaggtgt tgtgggcgtt cacgctcctg tggctcgcca tcctgccgac gcccggcgcc
     3721 aactcgctgc tcatcatcca gcttgccttg agcagtggct ggcgaagcgt ggccatcgcg
     3781 ctcagcggca atctcttcgg tattgcctgc tacgccttgg cgacgctgct cgggctggca
     3841 tggctactgg cggccgcgcc ttcggtgcgc ctcgccattt acgggctggg cggtctctat
     3901 ctggcgtgga cggggatccg gctggcagcg cgcggcctca aacgcggaca agcgcccgac
     3961 gccgcgccct cccaggcggt cgctgcctcg gccgccggca cgtttgcgcg aggcctgctg
     4021 acggcgctgg cgaacgtgca ggccctgttc ttcctcgcca gcatctttgc cagcgttggg
     4081 gttctcagcg cccctcttgc gacccgcctt gccgccatcg ccgtcatcgt catcggcaac
     4141 ggcgcctacc tgacgctact cgcctggctc atgcagaagc cggcagcgcg cacgttctac
     4201 caccgctacc agccgcgcat ggaagtggcg ttcggcgcgc tcttcctcct gttcggcgcc
     4261 cggctgctgg cgcgcgagtg ggcaggatgg ctctaggcca tcccgccagt tgcgctgagg
     4321 ctcaggactg cgcgtccacg cagacggtgg cctcgccctt gaagttcggc tgcgagcgat
     4381 acttcatttc gatctgcttg atggccctgc gctcgccccg cagatcgagg gggcgcgttt
     4441 ggccgccggc gcgaatttcg gagcgcaccg gaatatcatc gggctcgcca ttggcgtaga
     4501 tcaccttcag gtcgtccatg tagaccttgt tgccagagac ctgcaggcgg atggacttga
     4561 agcggccttc gccgcgcccg accctgatga tgtccttgtc ggtgatgaag ccgacctttt
     4621 gacagcccag ccgctcccag cgggcttcag ctgccgtcgg cgctatggcg agacagatga
     4681 gggctccggt ggcgatcgct ctcgttgcta gccgcatgca tattctccat cgctcaattc
     4741 atgctcccgt catgctccgg cggccggcgc cgcgggcgac ggctgggcag aagcggatgc
     4801 ttaacggctg atgggctagc cctccgtcgc acgacgcaca gtcgcttgcg tccgcaggca
     4861 gcgccgggcc cgcctcttcg cccaaattcc gacacactcg agggcgtgtc cgctgccggc
     4921 gcggcgcgca atggccgcca agcgtttgct cgcaccaagc ggcagcgccg ccgccgtcgg
     4981 cgcattgtgg cgagtggccg tcgccgcgac gtcgacacgg cagggcgagg agcttcgaga
     5041 cttcgaggct gcaaagcgca ccgagatcca agccattgct gctcggtggg ctattgcggg
     5101 gcctcgtcgt gctcggatgg cgcgcctggg agcgccggac gacttcggtc gaaatgaacc
     5161 tgtcgtcgat cgcagtcgcg aagcgttagg cggccggcgt gggggccgcc gtggcggcag
     5221 cccctcggag ttgtcgcagc aatccgccta gccgcgttcg cgacggcgct cgccgcgatc
     5281 acgatcgccg cgctcgcgcc gcggcgggcg tccaccgcca tcgaagcgtt cctcggcctc
     5341 gccttccgca cgcgggattt ccttgcccgt cgcctgatcg acgaccttca tggaaaggcg
     5401 caccttgccg cgatcatcga agcccagcag tttgacgtaa acctcctgcc cctccttgac
     5461 gacttcgccg acggtttcag gccgaccgtt ggtcagctgg gagatgtgga cgaggccgtc
     5521 cttggcgccg aagaagttca caaaggcgcc gaattccatg atcttgacga ccttgccctt
     5581 gtagacctgg cccacctcgg gttcggaggt gatgccgcgg atgcgcgcca aggccgcctc
     5641 gatggcttcg cgcttggccg cggcgatctt gatggtgccg tcgtcctcga tatcgatctt
     5701 ggcgccactc tcggccacga tctcgcggat cacggatccg ccggaaccga tgacctcgcg
     5761 gatcttgtcg acgggaatct tgatggtctc gatgcgcggc gcgtgttcgc cgagctcggt
     5821 gcgcgccgag cccaaggcct tcgacatctc gcccaagata tgcatgcggc cgtggtgtgc
     5881 ctgctccagc gccacgcgca tgatttcttc ggtgatgccc gcgatcttga tgtccatctg
     5941 cagcgaggtg acgcccttct cggtgcctgc caccttgaaa tccatgtcgc cgaggtgatc
     6001 ctcatcgccc aagatgtccg acagcacggc gtagtcgttg ccctccttga tgaggcccat
     6061 ggcgatgccc gccaccgggc gcttcagcgg cacaccggcg tccatcagcg ccaacgacga
     6121 gccgcagacc gtggccatcg aggaagaccc gttcgattcg gtaatttccg aaacgatgcg
     6181 cacgacgtaa ggaaattcct ccgcactcgg catcagcgga tgcaccgcac gccaggcaag
     6241 cttgccatgg ccgatctcgc gccgacccgg cgagcccatc cggcccgtct cgcccaccga
     6301 atagggcgga aagttatagt gcagcaggaa gcgctccttg cgcgtgcctt ccagggcatc
     6361 gacgtactgt tcgtcctccc cggtaccgag cgtggccacg acgagggcct gtgtttcgcc
     6421 gcgcgtgaac agggccgagc cgtgcgtgcg cggcagaacg tgcacttctg agcggatcgg
     6481 tctgacggtc gtcaagtcgc gcccatcgat gcggcgctgg gtgcggatga tatcgccgcg
     6541 cacgacctcc tgctcgagct ccttgaaaac cgcgccgagc agcacctctt cgccgccgtc
     6601 ggcttcgggc cccagcaact cggccttcaa tttggcattt gcggcctcga ccagttcatg
     6661 gcgcttctgc ttttccggcg tggcgaaggc ctggcgcaga tccgcctcga tcagcgcctt
     6721 ggccttggac ttgtacttgg atttgtcggc ggccttgaaa tcccacggct ccttggccgc
     6781 cttttccgcc aggcggatga tggcctggat gacgggttga aaaccgctgt gaccgtgcat
     6841 gacggcctcc agcatcaact gctcgggcag ctctttggcc tccgattcca ccatcatgac
     6901 ggcatcgccc gtacctgcca ccacgagatc gagatcgctc ttggccatct cgtcgatcat
     6961 ggggttcagc acgagcttgc cgtcgatcac gccgacgcgc gcgccgccaa ttggaccgag
     7021 gaagggcacg ccggagagcg tcagcgccgc cgacgccgct accagcgcca cgatatccgg
     7081 atcattctcc atatcgtgcg acagcacggt cgcaaccacc tgtgtctcgt gcttgaagcc
     7141 ctccacgaac aggggccgga tgggccgatc gatcaggcgc gaggtcagcg tttccttctc
     7201 ggtgggccgg ccctcgcgct tgaaatagcc gccgggaatc ttgccggcgg cgaacgtctt
     7261 ttcctggtag ttgacggtca aagggaaaaa atcaatgccg ggtcgttccg tcttctcggc
     7321 caccaccgtg gccaacacgc tggtctcgcc gtattgcgcc agcacggtgg catcggcctg
     7381 gcgtgccatc cgccccgtct cgagcaccag tttgcgcccg gcccagtcaa tttctacacg
     7441 ttgtgtgtca aacatctcgt atctcgtttc tcattgtgca aagcgccgag cgccggcacc
     7501 tgccgcggct caaagcgggc ccacgttgcg catgcgctgc gccgtgagcc gcgtggctgt
     7561 ccaatccctg gtccgctacc ggcggatgcc caaacgctcg atgatcttct gataacgtcc
     7621 ctcgtcgcgt gacttcacgt ggtcgaggag cctgcggcgc aagctcacca tcttgagcaa
     7681 accgcgccgc gaatgattgt cgttcttgtg aagcttgaag tgctcggtca gcccgttgat
     7741 gcgctcggta agcagcgcga tctggacctc gggcgaaccg gtatcattct tcttggtggc
     7801 gtattcctta acgagttgca gcttgcgctg tgagctcagc gacatcggct tgacctttct
     7861 tgtgtgctga agcgatcgag gcggcctcgg ccgggatgtc gtccagcctg gtcgcattgc
     7921 cgcagccttg gccgcggatg aggcgcgtta tacacgaact ggcgccgaat gcgaggaaaa
     7981 acgccgaacg caagaccttg atctaattca attatcccgc gtcgcccaga tgaaagacgc
     8041 ggatcggatg cagttcgccc cgcgcgatct cgccgagggc gacgagatga ccccggcaca
     8101 gggcataggt cgggcccggc tggtaggggg catcgcgtcc gcgcagcagg atcggttggc
     8161 cgttgagcag gcgcgcggcc tcggcctggc cgacattgag cacggcaaag tcgttgaggg
     8221 cggcctcgat cggcagcaac aggcgcagca gcgccgcctc gccgtgctcc agcgccaact
     8281 cgacgctatt caggctgatt gccgcggcct cctcgaaacc cgcaactcgc gtgcgacgca
     8341 gcgcgatcag atggcccagg cagccgagca cccgccccat gtcgcgggcg agcgcgcgca
     8401 cataggtgcc cttgccgcag cgcgcctcga acaccgcgct atcggcgtcg ggggtatcga
     8461 tgaggcgcaa gctgtggatc agcacgggtc gcggctccag gctcacggct tcgcccccgc
     8521 gcgccaaatc gtaggcgcgc cggccggcca ccttgatggc cgagtaggct ggcggcgtct
     8581 gcaggatctc gcccgtgaat tgggccaaca gccgcgaaat cgccgccgcg tcgctgcgct
     8641 gggtgctggt ggcgagcacg cggccttcgg cgtcgtcggt ctccgtctgt gccccccagc
     8701 gcaccgtaaa gcggtagcac ttctccccgt cgaccgcgta gggcacggtt ttggtggctt
     8761 cgccgaaggc gatcggcagc acgcctgtcg ccgccggatc gagggtgcct gcgtggccgg
     8821 ccttgcgcgc gccaaaggct cggcgcatgg cgccgaccgc ataggtggag gtcacgccgg
     8881 ccggcttgtc cagcaccagc caaccgttga ccgccttgcc ctttttgccc gacgcgcgcc
     8941 tcattgcttg tcgaggtcct ggcgcacctt gtcgctcgcc aacaggcggt cgatgcgcaa
     9001 cgcctcatcg aatgtggtgt ccgcccgaaa gcgcacctcg ggcgcgaact tcaggttgac
     9061 ggcgcgcgca acctcgccgc ggatgtagcg cctgtgacta tccaaggcgg ccaacaccgc
     9121 ctcgatgtcc ttgccgccga gcggcatcac gaaaatggtg gccaggcgca gatccggcga
     9181 catgcgcact tccgggatgg tcagcgcgtg cgcggccagc acctcgtcgt ggatctcgcc
     9241 gcgttgcaag agctcggcca gcgcatggcg cacgagttca ccgacgcgca gctgccgctg
     9301 cgatggacca gtgcttttgc cggaatggcg ccttcccatg gcctcaaaac tcgatcgcga
     9361 aaaagggtcg gctcacccgc gagccgaccc cctcaattgc gcaaaccgct tagagcgtgc
     9421 gggcgatggt ctcaacgttg aagcattcga tcacgtcacc ggcgcggata tcctggtagt
     9481 tggcaaacgc catgccgcac tcctggcccg ccgggacctc gcgcacctcg tccttgaagc
     9541 gcttgagcgt ggaaagcgtg ccttcgtgga tcacgacgtt gtctcgaatg agcctgacct
     9601 ttgcgccgcg ctccaccttg ccttcggtca cccggcaacc cgcaaccttg ccgaccttgg
     9661 agatgttgaa cacctccaaa atttgcgcgt tgccaagaaa gatctcgcgc agcgtcgggg
     9721 ccagcag
//
LOCUS       DASTMT010000509         3007 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_622793, whole genome shotgun sequence.
ACCESSION   DASTMT010000509 DASTMT010000000
VERSION     DASTMT010000509.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3007
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_622793"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1262
                     /locus_tag="VFR00_11730"
     CDS             <1..1262
                     /locus_tag="VFR00_11730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEU0159982.1"
                     /translation="EPHPLSTAIIKTVQYRRALYVAAFLHDIAKGRDEDHSIAGARIA
                     RHLAPRFGMSGAETETVAWLVEQHLAMSSFAFSRDIGDPKTIRDFADIVQSPERLKLL
                     LLLTVADIRAVGPSVWNGWKGQLLRALYYETEPVVAGGHTQLASRDRIAAAQAAFRTA
                     TADWARADVECFIERHYPDYWLKTDTRKVLEHAELIRSLESSGRKFGSSVATDAFTAI
                     TELSLFAPNHPRLLALFAGACAATGANISGAHISTTRDGFALDTFLLAREFDQDEDEL
                     RRARRISDTIEQLLKGEQRLGALMAKRRERRGRIGAFKVAPEVLIDNAISQQFTVIEV
                     AGLDRPGLLFELTNTLSDLNLDITSAHITTFGEKAVDVFYVTDLTNKKIVSPQRQKAI
                     RQRLLEVLGETSAGDPSLARSSSGRLP"
     gene            1315..2067
                     /locus_tag="VFR00_11735"
     CDS             1315..2067
                     /locus_tag="VFR00_11735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020179010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3750 domain-containing protein"
                     /protein_id="HEU0159983.1"
                     /translation="MVSIRMALATFALLFLAPLAVQAAWWLSHDSAVAWSEADWSSAQ
                     LLPLARAKPEALVHVYAARTGRWKGIFAHHSWIVIKERNATRYTRYDKVAWGRPVKID
                     GWAPDARWYGHRPVRIGAVEGAAAEALIPKVRAAVASYPHNDFGGYRVWPGPNSNSFV
                     SHVLCAIPEAKIALPPTAIGKDFRPEAALVALTPSRTGLQLSFAGLAGITVAWVEGVE
                     INVLGLVLGIDLRRPAIKLPGLGRIGVGMVVA"
     gene            2173..>3007
                     /gene="murJ"
                     /locus_tag="VFR00_11740"
     CDS             2173..>3007
                     /gene="murJ"
                     /locus_tag="VFR00_11740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013950382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HEU0159984.1"
                     /translation="MRLYRAFATVGGLTLVSRVFGFVRDILLAATLGTSAVADAFFVA
                     FRFPNLFRRLFGEGAFNSAFVPLFAKALEGEGKEAARRFAEEALAGLVLVLLLLTVVA
                     EIAMPILMWGLAPGFSADPAKYDLAVLLTRITMPYLLCMSVVALLSGVLNSIGKFVES
                     SSVSIVLNVTMMAATLFALAMGLRNDPVAGIIQAWGVFVAGLLQLWLLIDGARRYDLA
                     LKLRWPRLSQGVRRLIRLGIPGVIAGGVTQLNIMIGTIVASLQDGAVSHLYYADRIYE
                     LP"
BASE COUNT          480 a          983 c          947 g          597 t
ORIGIN      
        1 cggaaccgca cccgctctcg accgccatca taaagaccgt gcagtaccgg cgtgccctgt
       61 acgtggcggc attcctccac gatatcgcca agggacgtga cgaggatcac tcgatcgcgg
      121 gcgctcgcat cgctcgccac ctagccccgc gctttggcat gagcggggcg gagaccgaaa
      181 cggtcgcctg gcttgtcgag cagcatctcg ccatgagctc atttgccttc agccgcgata
      241 ttggcgaccc caagaccatc cgcgattttg ccgacatcgt gcaaagcccc gagcgcctca
      301 aactgctgct gcttcttacg gtggctgaca tccgtgccgt cggaccttcc gtgtggaatg
      361 gctggaaggg ccagctcttg cgcgcactct attacgagac ggaaccggtg gtggccggcg
      421 gtcacacgca gcttgcttcg cgcgatcgca tcgccgccgc ccaggctgcc tttcgcacgg
      481 caaccgccga ttgggcgcgg gcggatgttg agtgcttcat cgagcgccac tacccggact
      541 actggctcaa aaccgacacg cgcaaggttc tcgagcacgc cgagctgatc cgctcccttg
      601 aaagctccgg gcggaagttc ggcagcagcg ttgccaccga tgccttcacc gccatcaccg
      661 agctgtcgct gtttgccccc aatcacccgc ggctgctggc gctgttcgcc ggcgcttgcg
      721 ccgcgaccgg ggcaaacatc tctggtgctc acatctccac gacacgcgat ggatttgcgc
      781 tcgacacctt cctgttggcg cgcgaattcg accaggatga ggacgaacta cggcgggcgc
      841 ggcgaatctc ggacaccatc gagcaactgc taaagggtga gcagcggctg ggtgcgctga
      901 tggccaagcg acgcgaacgc cgcggccgga tcggcgcctt caaggttgcg cccgaggtgt
      961 tgatcgacaa cgccatttcc cagcaattca ccgtgatcga ggttgccggt ctcgatcgtc
     1021 cgggtctgtt gttcgagctc accaatactc tgtccgacct aaacctcgat attacctcgg
     1081 cgcatatcac cacctttggc gagaaggcgg tcgatgtgtt ctatgtcacc gatctcacca
     1141 acaagaaaat cgtcagcccg caacggcaaa aggccattcg ccaacgcctg ctcgaggtgc
     1201 tgggcgagac gagcgccggc gatccttcgc tggcacgatc ctccagcgga cggctgccct
     1261 agcgctgggg gcaggccttg atgtcggcaa attatcgcgt cagcagtgag ccctatggtc
     1321 agcattcgca tggcactcgc cactttcgcg ctcctctttc tggcaccgct ggccgtgcag
     1381 gcggcctggt ggctgtcaca cgatagcgcc gtggcctgga gcgaggccga ttggtcgagc
     1441 gcccaattgc tgcccttagc gcgggcaaag cccgaggcgt tggtgcacgt ctatgcggcg
     1501 cgcaccggcc gctggaaggg catttttgcc caccacagct ggatcgtcat caaggagcgc
     1561 aacgccacac gctacacgcg ctatgacaag gtggcgtggg gccggcctgt gaaaatcgac
     1621 ggttgggctc ccgacgcgcg ctggtatggg caccggccgg tccgaatcgg cgccgtggag
     1681 ggcgccgcgg cggaggccct gattcccaag gtgcgggcgg cggtggcgag ctatccccac
     1741 aacgattttg gtggctatcg ggtctggccc ggccccaact ccaattcatt cgttagccac
     1801 gtcttgtgcg caattccgga ggccaaaatc gcgctaccac cgaccgcgat cggcaaggat
     1861 tttcgccccg aggcggcgct ggttgcgctg acgccgagcc gaacaggcct gcagttgtcg
     1921 tttgccgggc tagccggcat taccgttgcc tgggtggagg gcgtggagat caacgtgctg
     1981 ggccttgtgc tgggcatcga tctacgccgg ccggccatca agctacccgg cctcgggcgg
     2041 atcggcgtcg gcatggtggt ggcctaaacg ccgcggcgtg gtggcctaag cgccgcggcg
     2101 aacccggcgc gcttgcggca gtttcgcact tgccttgctc ttgtccgcgg gctccgctag
     2161 gggtcgggcc ccatgagact ctaccgcgcc ttcgccacgg tcggcgggct gacgctagtc
     2221 agccgcgtgt tcggcttcgt ccgcgatatt ttgcttgccg ccaccttggg cacgagcgcc
     2281 gtggcggatg ccttttttgt cgcctttcgc tttccaaacc tgtttcgccg cctgtttggc
     2341 gaaggcgcct tcaactccgc ctttgtcccc ctgtttgcca aggcgctgga aggcgaaggc
     2401 aaggaggcgg cgcgccggtt tgcggaagag gccctggcgg gcctggtgct cgtcctgctg
     2461 ctgctgacgg tggttgccga gatcgccatg cccatcctga tgtgggggct ggcgccaggt
     2521 ttcagcgccg atccggccaa atacgacctg gccgtgctgc tgacccgcat caccatgccc
     2581 tatctcttgt gcatgtcggt ggtggcgctc ctctctggcg tcctcaactc gatcggcaag
     2641 ttcgtagaaa gctcctcggt ttcgatcgtg ctaaacgtca ccatgatggc ggccactcta
     2701 tttgccctgg ccatgggcct gcgcaacgat cccgtagccg gcatcatcca ggcctggggc
     2761 gtgtttgtgg cgggtctcct gcaattgtgg ctgttgatcg acggggcgcg gcgttacgac
     2821 ctagcgctca agttgcgctg gccgcgtcta agccaaggcg tgcgccgcct catccggctc
     2881 ggcatcccgg gcgtgattgc gggcggcgtc acccagctca acatcatgat cggcacgatt
     2941 gttgcttcgc tgcaggatgg cgctgtttca cacctctact acgcggaccg aatttacgag
     3001 ctgccgc
//
LOCUS       DASTMT010000510         1882 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_6055012, whole genome shotgun sequence.
ACCESSION   DASTMT010000510 DASTMT010000000
VERSION     DASTMT010000510.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 1882)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1882)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1882
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6055012"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..400)
                     /locus_tag="VFR00_11745"
     CDS             complement(<1..400)
                     /locus_tag="VFR00_11745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEU0159985.1"
                     /translation="MTAAQSHIHQLGEESSVWFKLTPLLKGPHNPLTILMQMAERYGG
                     VIPVNMASQRVVLLTAPEHFKRVLVTKADDYIKYFDGLKPIFGKSMITHDGVLWQKIR
                     MPQQPAFHPDMFAEYIPYFLAAIRSKMQTWA"
     gene            complement(645..1268)
                     /gene="purN"
                     /locus_tag="VFR00_11750"
     CDS             complement(645..1268)
                     /gene="purN"
                     /locus_tag="VFR00_11750"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013949726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEU0159986.1"
                     /translation="MAKKRLAILISGRGSNMMALVAAAQAPDYPADVAAIVSSRPDAA
                     GLAWARAQGLPASVIDHKIYPSREAFDAAVDQVLAEAGVELVALAGFMRIQSPQFVRK
                     WYLRQLNIHRSLLPAFKGLNPHAQALAAGVKISGCTVHFVGEDVDGGPIIAQAAVPVC
                     EGDTAQALAARILRAEHTLYPHALALVAAGRARLCDGRVTIDAADAT"
     gene            complement(1268..>1882)
                     /locus_tag="VFR00_11755"
     CDS             complement(1268..>1882)
                     /locus_tag="VFR00_11755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HEU0159987.1"
                     /translation="GAAERGELLPRPDIAAGDGLIGLTSSGVHSNGYSLVRRLVEAAG
                     TPWDAPAPFQAAGSLGAALLTPTRIYVKSVLKAMRETRAIKGLAHITGGGLTENLPRV
                     LPAGVAAHLNLAAWQGPAVFGWLQQASGLDDAEMLRTFNCGIGMVAVVAQSQIAAVMA
                     ALAAAGESPLLIGSLEPGTGVKAPAKGRGDAEAVRYSGRLRWTG"
BASE COUNT          304 a          624 c          635 g          319 t
ORIGIN      
        1 tggcccaggt ctgcatcttg ctgcggatgg cggcaagaaa gtaggggatg tactcggcga
       61 acatgtccgg gtggaacgcc ggttgctgcg gcatgcgaat cttctgccac agcactccgt
      121 cgtgggtgat catggacttg ccgaagatcg gcttcaggcc atcgaaatat ttgatgtagt
      181 cgtcggcctt ggtgaccagc acgcgcttga aatgctcagg ggccgtcagc agcaccacgc
      241 gctgactggc catgtttacg gggatgacgc cgccgtagcg ctccgccatc tgcatcagga
      301 ttgtcagggg attgtgcgga cccttcaaga gtggggtcag cttgaaccag accgaggact
      361 cttccccgag ttggtggata tgcgattgag cggcagtcat ctcgttgcgc tccggaggcg
      421 gtcgcggcgg caccacgtca ttccacgtca ctaaatgtac ccaccgtttg ggtgttcccg
      481 ccccgggccc ttgctcctcc ctaggtcttg acgtctcgag gggtttgttg ggtctcgtag
      541 caccgtcctc aaaactctgt ccagtttcgg cgctgcggcc aatgcgcttt cgttaacagg
      601 tgtggccacg attaacctaa cgcgaaggtt gcggtcgctc gcctctaggt ggcgtccgcg
      661 gcatcgatgg tgacccgacc atcacacagc cgcgcgcggc cggcggcgac cagggccaag
      721 gcgtgcggat aaagcgtgtg ctcggcccgc aaaatgcgcg cggccagggc ttgggctgtg
      781 tcgccctcgc acacgggcac cgccgcctgg gcgatgatgg ggccaccgtc gacgtcctcg
      841 ccgacgaagt gcaccgtgca gccggagatc ttgaccccgg ccgcgagggc ctgcgcgtgc
      901 gggttgagcc ctttgaaggc aggcagcagc gatcggtgga tattgagctg gcgtaagtac
      961 catttgcgca cgaactgagg gctctggatg cgcatgaagc ccgcgagcgc gacgagctcg
     1021 acgccggcct cggccaaaac ctgatcgacc gcggcatcga aggcctcgcg cgagggatag
     1081 atcttgtgat cgatgacgct ggccggtaat ccctgcgccc gcgcccaggc gaggcccgcc
     1141 gcgtccggcc ggctcgagac gatggcggcc acgtcggcgg gatagtcggg cgcctgcgcc
     1201 gccgccacca gcgccatcat gttggagccg cgccccgaga tcaggatggc gaggcgtttt
     1261 tttgccatca gccggtccac cgcagccgcc cgctgtagcg cacggcctcc gcgtcccccc
     1321 tgcccttggc tggcgccttc acgccggtgc caggctcgag gctgccgatc agcagcgggc
     1381 tttcgcccgc cgccgcgagc gcggccatga cggcggcgat ctgggactgg gcaaccacgg
     1441 caaccatgcc gatcccgcag ttgaaggtgc gcagcatttc ggcgtcgtca aggccgctgg
     1501 cctgttgcag ccaaccaaac accgccgggc cctgccaagc agcgagattg agatgggcgg
     1561 ccacgccggc gggcaggacc cgcggcagat tttccgtcag gccacccccg gtgatatggg
     1621 cgaggccctt gatcgcccgc gtctcccgca tggccttgag cacgctcttg acgtagatgc
     1681 gcgtgggcgt caacagcgcc gccccgagcg agcctgccgc ctggaacggc gcgggggcat
     1741 cccagggggt gccggccgcc tcgaccaagc ggcgcaccag cgaatagccg ttggagtgaa
     1801 ctcccgagga ggtgagcccg atcaggccgt cgccggcagc gatatccggc cgcggcagca
     1861 gctcaccgcg ctcggccgcc cc
//
LOCUS       DASTMT010000511         1808 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_2263034, whole genome shotgun sequence.
ACCESSION   DASTMT010000511 DASTMT010000000
VERSION     DASTMT010000511.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 1808)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1808)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1808
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2263034"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..560)
                     /locus_tag="VFR00_11760"
     CDS             complement(<1..560)
                     /locus_tag="VFR00_11760"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU0159988.1"
                     /translation="MSEAQLQDHIILVTGALGTLGAAMCRAIEAAGGLAIRSDLAARG
                     KVDLALDVTREEDWKAAMAEIERRHGRLDGLVNNAGVGTPGDVEQTSFAEWRRVMAVN
                     ADGVFLGCKYAMALLAKAKAPAIVNISSASGIVGGANLAAYNASKGAVRLLTKSVALS
                     GARKRPPVRCNSVHPAFIEGDMVDRMV"
     gene            complement(557..826)
                     /locus_tag="VFR00_11765"
     CDS             complement(557..826)
                     /locus_tag="VFR00_11765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0159989.1"
                     /translation="MLFWAGAVLTGILGYKQLDWWVAGGIACAVAAAQFLLFQNLLGT
                     QGAGLELLFGLALNLTMYTATFSIGRAIGQRRARRRQRKATRGRP"
     gene            1089..>1808
                     /locus_tag="VFR00_11770"
     CDS             1089..>1808
                     /locus_tag="VFR00_11770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012042976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase"
                     /protein_id="HEU0159990.1"
                     /translation="MPSLADYMSVAASLPRDGSAGALAGRVWLPMAEGPAVVAVRGEE
                     LYDISKLTPTMRDLCEAERPAEIVRAGKGEPIGALADVLGNTPAARREPAKPWLLAPI
                     DLQAIKAAGVTFPRSMLERVIEEQARGAPEKAEALRKSVAAHFGGNLANLVPGSKEAA
                     ELKQVLIAQRAWSQYLEVGIGPDAEIFTKAQPMSAVGHGMDAGLHPISTWNNPEPEVV
                     LLVSSGGAIVGATLGNDVNLRD"
BASE COUNT          294 a          639 c          592 g          283 t
ORIGIN      
        1 accatgcggt cgaccatgtc gccttcgatg aaggcggggt gcaccgaatt gcagcgaacc
       61 ggcggccgct tgcgcgcacc cgacagcgcc acggatttgg tcaacaagcg caccgctccc
      121 tttgaggcat tgtaggccgc aaggttggcg ccgccgacaa tgccggaggc cgaagagata
      181 ttgacgatcg caggcgcctt ggctttggcc aacaacgcca tggcatattt gcagccgagg
      241 aacacgccgt cggcattgac ggccatcacg cggcgccatt cggcaaaact cgtctgttcc
      301 acgtcccccg gcgtgccaac gccggcattg ttgacgaggc cgtcgagccg accgtggcgg
      361 cgctcgatct cggccatggc cgccttccag tcctcctcgc gcgtcacgtc gagcgcgaga
      421 tcgaccttgc cgcgcgccgc caggtcgctc ctgatggcga ggccgcccgc cgcctcgatc
      481 gcccggcaca tggcagcgcc aagcgtgccg agcgcacccg tcaccaggat gatgtggtct
      541 tgaagttgcg cctcactcat ggtcgacccc ttgtggcttt cctctggcgg cgccgggcgc
      601 ggcgctggcc gatcgcccgg ccgatggaga aggtggcagt atacatcgtc aggttcaggg
      661 ccaggccgaa cagcagctca agccctgcgc cctgcgtgcc gaggaggttt tgaaacagca
      721 agaactgcgc ggcggcaacg gcgcaggcga tgccccctgc cacccaccag tctagctgct
      781 tgtagcccag aatacccgtc agaacggcac ctgcccaaaa cagcaaacaa gcctcctcgg
      841 cgcagcgccc ccgtggccgt ggcgcggccc gtcgcaatgg cttgagcccg gccgaatgca
      901 gcgccggacg ccggagtgcc ggcagctcgc cccggttgtc tcacgtttgc tgggcgaagc
      961 aagccttctg gcaccttcgc cgccaagccg ctagtgtctt tcgctcaaga ccgcgccgag
     1021 cgcgctcgac ttcgccgcgc cgttggcaat cggggcctgc ggccttgtca gtttcacgaa
     1081 gggtgcccat gccgagcctt gccgactaca tgtccgttgc cgcgagcctg ccccgcgacg
     1141 gcagcgccgg cgcgctcgcc ggtcgcgtct ggctgccgat ggccgagggt ccggccgtcg
     1201 tcgcggtgcg cggggaggaa ctctacgaca tctccaaact gacgcccacc atgcgcgatc
     1261 tgtgcgaggc cgaacggccc gcggagatcg tgcgcgcggg caagggtgag cccatcggcg
     1321 cgctggccga cgtcctcggc aatacccccg cggctcggcg cgaaccggcc aagccgtggc
     1381 tcttggcgcc gatcgacctg caggccatca aggcggccgg cgtcactttt ccccgctcga
     1441 tgctggagcg cgtgatcgag gagcaggcgc gcggggcgcc cgagaaggcg gaggccctcc
     1501 gcaaaagcgt cgcggctcac ttcggtggca accttgccaa cctggttccc ggcagcaagg
     1561 aggccgccga gctcaagcag gtgctgatcg cccagcgcgc ctggtcgcaa tatctggagg
     1621 tcggcatcgg ccccgacgcg gaaatcttca ccaaggcgca gccgatgtcg gcggtgggcc
     1681 atggcatgga cgcgggcctg catccgatct ccacctggaa caatcccgaa cccgaagtgg
     1741 tgctgctcgt ttcttcgggc ggcgccatcg tcggtgccac gctcggcaac gacgtcaacc
     1801 tgcgcgat
//
LOCUS       DASTMT010000512         4570 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_4227535, whole genome shotgun sequence.
ACCESSION   DASTMT010000512 DASTMT010000000
VERSION     DASTMT010000512.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4570)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4570
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4227535"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..483
                     /locus_tag="VFR00_11775"
     CDS             <1..483
                     /locus_tag="VFR00_11775"
                     /inference="COORDINATES: protein motif:HMM:NF024820.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU0159991.1"
                     /translation="GWRMLGWSYFQTGRYKEAAGAYAKAVDLDPSSAELKLAYEEAKA
                     KAAAADTLETAAASQTESIARAADGASVGIPGQADAGQSAHPDALIRAMVDGLAARLE
                     SSPRDVEGWARLIRSRVVLGEREVAATALRKALEVFKDDPAATRKIAAGAVELGLKTE
                     "
     gene            769..972
                     /locus_tag="VFR00_11780"
     CDS             769..972
                     /locus_tag="VFR00_11780"
                     /inference="COORDINATES: protein motif:HMM:NF041374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDCCVxC domain-containing (seleno)protein"
                     /protein_id="HEU0159992.1"
                     /translation="MELLSVITCPACSYKALEVMPTDACRYIYDCKRCSYRMRPIKGK
                     CCVFCSYGSVSCPPVQNERACFG"
     gene            1344..1895
                     /locus_tag="VFR00_11785"
     CDS             1344..1895
                     /locus_tag="VFR00_11785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0159993.1"
                     /translation="MLISKTLLVMASGLALATPALAQDHAAAFEYSAVVRLTRSGQFQ
                     AEVWRRDVHTDNADLAWTNRELYPTAAVAMGEACASLRKNFDLTFSCLRAPPQGLPKT
                     KAPTAPNVTAAAPKSAVPTPERKVTGNEQVTSNAIAPAWLKDFWKVQERQSGGAGGGG
                     GGGGGGGGGGGGGGGSGGSGGGY"
     gene            2139..2267
                     /locus_tag="VFR00_11790"
     CDS             2139..2267
                     /locus_tag="VFR00_11790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0159994.1"
                     /translation="MGSGKGDEGDEREAALGATDEIGTAANVGPHAWQKNCASGEL"
     gene            complement(3074..3313)
                     /locus_tag="VFR00_11795"
     CDS             complement(3074..3313)
                     /locus_tag="VFR00_11795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0159995.1"
                     /translation="MKKVLLWLGALAVAAGLTFAAAPSGQAAPAQSALPGLESIDEGA
                     STPIHCRRYRHCHDRCVRRWAGVCRKRVTKYCHRC"
     gene            complement(3702..3776)
                     /locus_tag="VFR00_11800"
     tRNA            complement(3702..3776)
                     /locus_tag="VFR00_11800"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3742..3744),aa:Lys,seq:ttt)
     gene            4043..>4570
                     /locus_tag="VFR00_11805"
     CDS             4043..>4570
                     /locus_tag="VFR00_11805"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEU0159996.1"
                     /translation="MTETASPALPMVSRPLKAIALMCVAWVLFACLDATAKYLAAVVG
                     MPAAQAVWMRFLGQFVAMVVALGLLAVPRLMRTNKLKVQIVRSFLLLSSTAFNFLALR
                     HLRLDQTTTIGFLTPLTVALLAGPFLGEWISWRRAIAIAIGFAGILVALRPGFTAVHY
                     AFAFSLAGMLSYAVFS"
BASE COUNT          814 a         1463 c         1479 g          814 t
ORIGIN      
        1 ggctggcgga tgctcggctg gtcctatttc cagaccgggc gctataagga ggcagctggc
       61 gcctatgcca aggcggtaga ccttgatcct agctcggccg aactcaagct cgcctacgaa
      121 gaagccaagg cgaaggccgc cgccgccgac acgctagaga ccgcggcggc ttcgcaaacc
      181 gagagcatcg ccagggctgc tgatggggca agcgtcggca tccctggtca agccgacgcg
      241 ggtcagtctg cccacccgga cgctctcatt cgcgcaatgg tggatggctt agcagcgcgt
      301 ctcgaaagtt cgccgcggga tgtcgagggc tgggcccgcc ttatccgctc ccgagtggta
      361 ttgggtgaga gagaggttgc cgcaacggcg ttgcgcaagg ccctcgaggt cttcaaagac
      421 gacccggccg caacccgcaa gattgccgcc ggggccgttg aacttggcct gaagaccgaa
      481 tgacctgcgg gttttacctt cgtgccttag agatgcgaac tgccggggcg atcgcacgag
      541 ctgagcgccc gggtgtccaa gcgcgccgct gggagcacgg catcccgcgc cgccaacgcc
      601 agtcgcccct gctggtgctg cagggccggc gtgccggcgc tcaacaccat gagcccgcag
      661 cgaccgggag cggccaaatg gttctaagtt ctcggccttt ggtccgattg agccccccgc
      721 agttacggga tttaactttg tagttccgtc gagatgcggg gacgatccat ggaattgctc
      781 tctgtgatta cttgtccggc ctgcagttac aaagcgctcg aagttatgcc gacagacgcc
      841 tgtcgctaca tttacgactg caaacgctgc agctatcgca tgcgacccat taaggggaag
      901 tgctgcgtat tttgctccta cggatcagtg tcgtgcccgc cagtgcaaaa cgagcgcgcc
      961 tgcttcggct gagcggcttg gtgctgcacg ggcggcacgc acgcgcaagc agcacctctg
     1021 agccgccggc agctgttgct ctttgcccgc atggcggcat tgcaccacag cggtgggctt
     1081 cgcgatagcg atcagggctg catcaccggc cttcggcgtg ccgccgtatc tgtcgccgtg
     1141 atcgctgacg atccgatggg gatcgtacac gcaggagccg accttacagc cgttgtcgtg
     1201 caagatcggt cgtcatacgc ggccgcggca gagccaagaa aaagaagatc gcctttgcgg
     1261 ttggactcgt cacacaatgg ccagagttgg cgatctagct gacgttgtgc ggggttttca
     1321 gatgagcacc tcagggggaa tacatgctca tttcaaagac cctccttgtc atggcgagcg
     1381 gcctcgcact cgcaacgccg gcactggcgc aggatcacgc cgccgctttt gagtactcgg
     1441 cagtggtgag gctcacgcga agcgggcaat tccaagcaga ggtctggcgg cgcgatgttc
     1501 acacggacaa cgccgacttg gcttggacca atcgtgagct ctaccccacc gccgcggtag
     1561 ctatgggcga ggcgtgcgcg tcgttgcgca aaaactttga tctcacgttc tcttgtctgc
     1621 gcgcgccccc gcagggtctt cctaaaacca aagcgcctac cgcaccaaac gtcacagccg
     1681 cagcaccgaa gagcgcggtt cccacgcccg agcgcaaggt gaccggtaac gagcaagtga
     1741 cttcgaatgc gatcgccccc gcgtggctca aggacttctg gaaggttcag gagcgccagt
     1801 ctggcggcgc cggtggtggc ggcggcggtg gtggcggagg tggaggcggt ggtggtggtg
     1861 ggggcggcag cggaggtagc ggcggcggct actgagatct cgcgggcacg tcacggcttg
     1921 cgtgcgtgta tgatcctcct cgccacgctc gggcgtacgg ggctttccgc aatcggccaa
     1981 gcgcgcggcg cgaccggaca ggaatgcatg gcgcgatgag aggcgcgctg cgcggcttcg
     2041 catcggtgag cacaaagacg gccagccgta cgccatccac ggcgccaaga aaatcacgct
     2101 cttgctcagc agcaagaggc ggccgatcgc agcggcgctt gggcagcggc aagggcgatg
     2161 aaggcgacga gcgcgaggcg gcgctcggcg cgaccgacga aatcggcacg gcagcaaacg
     2221 tggggccgca cgcttggcaa aaaaattgcg cctcgggcga gctgtgaccc cggggcgcag
     2281 ctcttattga gcaatccaca caccccaact gacgggggga aggtctgcgg tctgtacgat
     2341 ctccgatagt gccacccact gtcccacgcc ttggcccaaa tcaagacccg agacgcaaca
     2401 aggcggctct cctatgctca aagcgaaggc ttgcgcgacg acccggttca gggtccgcca
     2461 ccgcggccgt cgcgatccat ctcctgccac caagcgcttg agcaaaccgc gggtccgccg
     2521 cacggggccg ccgcactgag gaccaagagc aaaagcgtgg cgatgttgcc ttttgtttgg
     2581 cctccttgcg ggctgtttgt ctcgacggct atggccattg cctgcctagc ctgcaggttt
     2641 tggcgccggc actgcggccg tcaccttagc tgctgaggtg gaaacggttg gcgacagacg
     2701 ccctagggcg ccacccgcgg acgatgcagc ggacacgcga cacacaggcg aaggaggttc
     2761 gggataacct gttccaggcg ccttcagccc cccgccgccc tgccaccctt gtcgggtttg
     2821 gatgtgggcg aggcgctggg aacctattgc cgccgtctcg catccaatgg gtgtaagtcg
     2881 ccttggggac gctaagaaca cgcagcgaag cgttgccctg gtatccccaa tcctcccgag
     2941 aagcactcgg ccgcgcggcc gggtgcttcc gagggggtgc gacgctatgc gtgcgagcca
     3001 acctcaattc gtcagcgcaa acagcgaacc gggcggcgag cgcggcccgc gccgctcgga
     3061 gcttgccggc acgctagcag cgatggcagt acttcgtcac gcgcttgcgg cagacgccgg
     3121 cccaacgccg cacgcagcgg tcatggcagt gccgatacct tcggcaatgg atcggcgtgg
     3181 acgccccctc atcgatgctc tcaaggcccg gcagggccga ttgcgctgga gccgcttggc
     3241 cgctcggcgc cgcggcgaag gtgaggcctg ctgcgaccgc gagggcgcca agccagagca
     3301 gcactttctt catcggtgtc ctccgttcag ctgcattttg aaggcgcgaa gcctaggctg
     3361 aagttgccgg cgcagcaatc aacaccgcgg cgggcgaacg ccttcgccgc aaccgcgttg
     3421 cctcaggaag cgtgagcgcg cggacccgcc ccgcacccgc ggctggacgc cgtggcatca
     3481 ggaacgattt gccggcgatg atcccgatcc cctgcgagcg gggaatgccc gaaaaatcgc
     3541 cccgctaggc tcgcggcgat cgccgtgcgg ttgcggtcgg ccggcagggg gatgcatcga
     3601 acaggaacag aacgaaaagg cgctgggaat ttccaaagcc atatggctcg acagcccatg
     3661 acggagaaga attccgtcgt tgtgcgccaa cccctggcaa gtggtgagcc cggttgggat
     3721 cgaaccaacg accacctgat taaaagtcag atgctctacc gctgagctac gggcccacta
     3781 aacccgcgtg gttactggca atcccgcgcg aatgcaagtt ggcagcaggc ccaaagtggc
     3841 tcgcccggcg caaattgtcg cttcggagcg ggcgaggggg tgcagcgcgg gttccatgcg
     3901 ttcgaatctt gtgctttttc cacacgctgt ccgaggcatg cgtttgcaac atagcctacc
     3961 tcgtgcgcga ccggtcgccc ttctgccccg ggccctataa gagaacagcg cgatgcctcg
     4021 accccatgct gcaatcccca caatgaccga aaccgcctcc cctgccctgc cgatggtgtc
     4081 tcggccgctc aaggcaatcg cgctcatgtg cgttgcttgg gtcctctttg cctgtctcga
     4141 tgccacggca aaataccttg ccgccgtcgt cggcatgccc gccgcgcagg ccgtgtggat
     4201 gcgatttctc ggtcagtttg tggcgatggt ggtcgcgctc ggtttgttgg ccgtgccgcg
     4261 tctcatgcgc accaacaaac tcaaggtgca gatcgtgcgc tcatttctgc tgctgtcgtc
     4321 cacggccttc aatttcctgg cgctgcgcca cttgcgcctc gatcaaacga caaccatcgg
     4381 gttcctgacg cctctgacgg tcgccctgct ggccggcccg ttcctgggcg aatggatcag
     4441 ctggcgccgc gcgatcgcca tcgccatcgg ctttgccggc attctggtcg ccctgcgccc
     4501 cggtttcacg gcggtacact atgcgttcgc gttctcgctg gcgggcatgc tgagctatgc
     4561 ggtgttcagt
//
LOCUS       DASTMT010000513         6382 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_4742927, whole genome shotgun sequence.
ACCESSION   DASTMT010000513 DASTMT010000000
VERSION     DASTMT010000513.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 6382)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6382)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6382
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4742927"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            131..1651
                     /locus_tag="VFR00_11810"
     CDS             131..1651
                     /locus_tag="VFR00_11810"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEU0159997.1"
                     /translation="MASQPTWARPPQVALIILAYLAIHFGVRLWIGPTLGLDDAEQAL
                     FAQQWLLNYRFRAPPLFTWALLGTSHLSDINALTISLLRYLLFAMIAGFTYLTARWLI
                     RDARLAALATFSFAAIYVFGYYSHHDLTHTTVLSAFLAASWYAFVRLCEKPSLPRYLA
                     LGAAMGFGLLGKWNFFMFAAALPLAALTEPRERALLFNWRILPAALLTAAIALPSALW
                     ALHVGPAAGDGLGALLGEPARSWLGGLLLGSADLALAVLVYPMPFLAIFLALLGSAAW
                     RGFNAPEMLPEPVVSARFLGRVMAIAVLLHWLLVPLAGATNFSERLLQPALQILPIYL
                     FMLVERGQAADRALSIYAVLLLTTVAVALAARILVQAACPGACRTLVPFQAVAEELRA
                     AGFAGTGTIVVSDFHLGGNLRLHFPRARLIDIGYPPRVWPAPSGQGQCLALWPVKASM
                     DGTFDRYLSRELGVPVDAPRREGEVNVRLSSLLGGAKTRSYRLLYRLYEPSQGDCR"
     gene            1648..2649
                     /locus_tag="VFR00_11815"
     CDS             1648..2649
                     /locus_tag="VFR00_11815"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU0159998.1"
                     /translation="MSDTLTGMVASTGRTREAHALSIVIPAKDEEESLPLVVARILAA
                     GAASGQIVRDIVLVDDGSSDNTWGVMAELAADNEVIQAIRLRRNFGKATALMVGIGAC
                     RGDVIITMDADLQDDPDEIARFVETLDRGYDLVSGWKKERHDPLNKTVPSRLFNKVTA
                     AISGVKLNDFNCGFKAYRREIFDAIQLYGELHRYVPVLANALGYRIAEIPVRHHARRF
                     GTSKYGVARYLRGFLDLLTVVLITRFAYRPGHLFGSIGSLLAAGGSVALGYLIALKVL
                     TGASIGNRPLLLLGVMAVIVGVQLILFGMLAELIIHRTQRPVEMRHLVAEQRGQKRT"
     gene            2646..3671
                     /locus_tag="VFR00_11820"
     CDS             2646..3671
                     /locus_tag="VFR00_11820"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HEU0159999.1"
                     /translation="MRRFLTALAAAAITALCLWFLLTPQVLLALGRLSTQAKLAPLLA
                     AFVLAALVQWLRAWRFAVLTTGRPALPDAAMLAIACKLNFLNFVLPFRLGELGYPAMM
                     RQQYGHSLLRSTGVLLTARLFDLTTVLAILLSAAALAISTPAGQLALSAGALLFALSP
                     FALAGLGAALLIRLGSPLARLELRLSSPGAGRTAKLGADLAGRLRSFEPAARHVAHLA
                     AVAHGPLRLVVLLGFAIWLAFAAAAILVAGAVVDSVGPAAALLGAAAGNLAFALPING
                     IAGLGPAQAAWVVATTWAGVPQPDAVLSALALHAVVLSNALLLGGLALTGGFGRRPAL
                     RQRQPSP"
     gene            3910..4449
                     /locus_tag="VFR00_11825"
     CDS             3910..4449
                     /locus_tag="VFR00_11825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014328664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HEU0160000.1"
                     /translation="MSLLLRFSRGIDWFNAQFGLVANWLVLFACLISAGNAASRYLFS
                     ESSNGWLEIQWYLFAGMVLLGAPYTLKMNEHVRVDLVYGMVSERTRIWIDIVGGLLFL
                     LPICLILTYFTWPWFVESFVGNEGSSNAGGLIRWPVKLLLPVGFTLMALQGLSEIIKR
                     VAAIEHLTDEKFTYEKPLQ"
     gene            4446..5861
                     /locus_tag="VFR00_11830"
     CDS             4446..5861
                     /locus_tag="VFR00_11830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019996297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEU0160001.1"
                     /translation="MSFIAHNMAPLMFGGMILFMLIGYPAAFSLAATGLFFGFLGIEL
                     GLIRPDFLGNLTYQLFGIISNDLLLAIPFFTFMGAILERCGLAEDLLDSIGQLFGPVR
                     GGMSYAVIFVGAILGAITGTVAASVIAMGVISMVPMLKYGYSTRHTMGVIAASGTITQ
                     LIPPSLVLVVLADVLGRSVGDMYAGAIGPSFIQVALFCAWVAIVSVLWPKDVPALPLE
                     ARTLSGSALWFKCLRGMIPSLGLIFLVLGTIFMGLATPTEAGAMGVVGAIALAASYGT
                     LTWKLLYQGMASTMRLTAMVVYILIGARVFSLVFQGVGGKEWIEHLLTSLPGGQVGFL
                     VFVNAFIFIVAFFLDFFEIAFIIIPLLAPVADKLGIDLVWFGVLICANIQTSFMHPPF
                     GFALFYLRGIAPPSIKTSDIYWGAIPWVFLQVILVIIVIFWPGSVTYWIEKGTGVDPS
                     KVEINVPMPTLPPAFDPSQFK"
     gene            6017..6253
                     /locus_tag="VFR00_11835"
     CDS             6017..6253
                     /locus_tag="VFR00_11835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0160002.1"
                     /translation="MRTKMVKVIAFWGIISIVCAFIAGIVAGLKRRDHSFWAAWSFLF
                     PPMLLLLLVMRPNRGPRPRRPGLDELEADDRRFL"
BASE COUNT          851 a         2184 c         2001 g         1346 t
ORIGIN      
        1 gagggcgcgt gccagttggc gcgcgtgcca cagtcccccg tgtcccacga cccacggtcc
       61 ctggccgttg ggtggcggtc ggcggcggga taccctataa cgggtcgcca accgtcaacc
      121 tcggtcctgc atggcatcgc aacccacctg ggcgagacca ccgcaggtcg ccctcatcat
      181 tctcgcctat ctagccatcc atttcggcgt gcgcctgtgg attgggccaa cgctcgggct
      241 cgatgatgcc gagcaggcgc tgttcgccca gcaatggctg ctcaattacc gcttccgggc
      301 gccgccgctg ttcacctggg ccctgctcgg gaccagtcat ctgagcgata tcaacgccct
      361 gaccattagt cttctgcgct atctgctgtt cgccatgatc gcgggcttta cctatttgac
      421 cgcccgctgg ctcattcgcg atgcgcgcct tgccgcgctc gccaccttca gtttcgcggc
      481 gatctacgtg ttcggctact acagccacca tgatctgacc cacaccaccg tgctctcggc
      541 ctttctggcg gccagctggt atgccttcgt gcgcttgtgc gaaaagccct cgctgccgcg
      601 ctacctcgcg ctcggtgccg ccatggggtt cggcctgttg ggcaagtgga attttttcat
      661 gtttgccgcc gccctgccgc tcgccgccct gacggagccg cgcgagcggg cgctcctgtt
      721 caattggcgc atcctccccg ccgccttgct gacggcggcg atcgcgctgc cctcggcact
      781 gtgggcgctg catgtggggc cggcagcggg cgacggcctg ggcgcacttt tgggcgagcc
      841 cgcccgcagc tggctgggtg gcctgctgct gggctccgcc gacctggcgc tcgccgtcct
      901 cgtctacccg atgccgtttc tggcgatctt tctggccctg ctcggatcgg cggcatggcg
      961 cggcttcaac gcgcccgaga tgctgccgga gccggtcgtc agcgcgcggt tcctgggccg
     1021 cgtcatggcc attgcggtgc tgttgcactg gctgctggtg ccgctcgccg gggccaccaa
     1081 cttctccgag cggctgctgc agccggcgtt gcagatcttg cccatctatt tgttcatgct
     1141 ggtggagcgt gggcaggccg ccgaccgcgc gctctccatc tacgcggtgc ttctgctaac
     1201 caccgttgcc gtcgccctcg ccgcgcgcat tctggtgcag gcggcctgtc cgggcgcgtg
     1261 ccggacgctc gttccgtttc aggcggtggc cgaggagctc cgggccgccg gctttgccgg
     1321 cacgggcacc atcgtcgtct ccgattttca tcttggcggc aatctgcgcc tgcacttccc
     1381 gcgcgcccgg ctgatcgata tcggctaccc gccacgcgtc tggccggcgc cctccgggca
     1441 ggggcaatgc ctggcgctat ggccggtcaa ggccagcatg gatggcactt tcgatcgata
     1501 cctcagccgg gagctgggcg tgccggtcga tgcaccgcgc cgcgagggcg aggtgaatgt
     1561 gcggctctcg agcctgctcg gcggcgccaa gacgcgcagc tatcgcctgc tctatcgact
     1621 ttatgagcca agccagggtg attgccgatg agcgacacac tgacgggaat ggtggccagc
     1681 accggtcgca cgcgtgaggc gcatgcgctc agcatcgtca tccccgccaa ggacgaggag
     1741 gagtcgctgc cgctggtggt ggctcgcatc ctggcggccg gcgcggccag cggccaaatc
     1801 gtgcgcgaca tcgtgctcgt cgatgacggc agcagcgaca acacctgggg cgtgatggcc
     1861 gagctcgccg ccgataacga ggtgatccag gccattcgac tgcggcgaaa tttcggtaag
     1921 gcgaccgcgc tgatggtggg catcggcgcc tgccggggtg acgtcatcat caccatggat
     1981 gccgatctgc aggatgaccc cgacgagatt gctcgcttcg tggagacgct cgatcgcggc
     2041 tacgacttgg tgtcgggctg gaagaaggaa cgccacgatc cgctcaacaa gaccgtgccg
     2101 tcgcgccttt tcaacaaggt gacggccgcc atcagcggcg taaagctcaa cgatttcaat
     2161 tgcggcttca aggcctaccg ccgcgagatc ttcgatgcca ttcagctcta cggcgaactg
     2221 caccgctacg ttcccgtgct cgccaacgcg ctcggctatc gcattgctga gatcccggtg
     2281 cgccatcacg cccgtcgctt cggcacctcg aagtacggcg tggcccgcta cctgcgcggt
     2341 ttcctcgatc ttctgacggt tgtcttgatc acgcgcttcg cctaccggcc cgggcacctg
     2401 ttcggcagca tcggcagctt gctggcggcg ggcggcagcg ttgcgctcgg ctatctgatc
     2461 gcgctcaaag tgctgactgg cgccagcatc ggcaaccggc cgctgctgct gttgggtgtg
     2521 atggccgtta tcgtcggtgt gcagctgatt ttgttcggca tgctggcgga gctcatcatc
     2581 catcgcaccc aacgtcccgt ggaaatgcgc cacctggtgg ccgagcagcg cggacaaaag
     2641 cgcacatgag gcgcttcctg acggcgctcg ctgcggcggc catcaccgct ctctgtctct
     2701 ggttcctgct gacaccgcag gtgctcctcg cgctgggccg gctgagcacc caggcgaaat
     2761 tggctcccct actggccgcc ttcgtcctgg cggcgctggt gcaatggcta cgcgcctggc
     2821 gcttcgccgt gctgaccacc ggccggccgg cgcttcccga tgcagccatg ctcgcaatcg
     2881 cctgcaaatt gaactttctc aatttcgtct tgccctttcg cctgggcgag cttggctacc
     2941 cggccatgat gcgccagcag tatggtcact ctctcttgcg ttcgaccggc gtcctcctca
     3001 ccgcccgcct gtttgatctg acgacggtgt tagccattct gttgagtgcc gcagcgctcg
     3061 ccatcagcac gccggccggc cagctggcct tgagcgccgg cgccttgctg tttgcccttt
     3121 ccccctttgc gctcgcgggt ctgggcgccg cgctcttgat ccgcctgggc tcgccgctgg
     3181 cccgcctcga gctccgcctc tcgagccccg gcgcgggacg gaccgcaaaa ctcggcgccg
     3241 acctagccgg acgtctgcgc agtttcgagc cggccgcgcg ccacgttgcc catcttgccg
     3301 ccgtcgccca tggccccttg cgcctggtgg tgctgttggg ctttgccatt tggctggcct
     3361 tcgcagctgc ggccatcttg gtggcgggtg ccgtggtgga cagcgtggga cccgcggcgg
     3421 cactgttggg ggccgcggcg ggcaacctcg ccttcgccct gcccatcaac ggcattgccg
     3481 gtctcggtcc ggcgcaggcc gcctgggtgg tggcgaccac ctgggccggg gtgcctcagc
     3541 ccgacgcggt cttgagcgcg ctggccctgc atgccgtggt gctgagcaat gcgctcctgc
     3601 tcggcggcct tgccctgacc ggcggtttcg gccgccggcc ggcgctgcgc cagcgccagc
     3661 cctccccttg aagggggcgc cggctcgcac tggccagagg ggccgggccg cggccttctg
     3721 ccgggaccgt ttcggcggcc caagcccttc cgtctgcgac cagtttcggc taacgctagg
     3781 gccgggccac ggccgctcgt gccgcacccg ccggggggaa atcttgccac ggacattagg
     3841 cgttgctcgc tttcgcgcgc gatccgctgc ggccgccctt cggcttcacg ccgcctgctg
     3901 agaggaatga tgtcccttct tttacgcttc agccggggca ttgattggtt caacgcgcaa
     3961 ttcggcctgg ttgccaattg gctggtgctg tttgcctgcc tgatcagcgc cggcaatgcg
     4021 gcgagccgct acctcttcag cgagagctcg aacggctggc tcgagatcca atggtacttg
     4081 ttcgctggca tggtgctgtt gggcgcgccc tatacgctga agatgaacga gcatgtgcgc
     4141 gtcgatctcg tctacggcat ggtctccgaa cggacgcgca tttggatcga catcgtcggc
     4201 ggcctcttgt tcctgctgcc gatctgcctg atcctgacct atttcacgtg gccatggttc
     4261 gtcgagtcgt tcgtcggcaa cgaaggttcg tccaatgcgg ggggactgat ccgttggccg
     4321 gtgaaactgc tcctgcccgt tggcttcacc ctgatggccc tacagggcct ctccgagatc
     4381 atcaagcgcg ttgcagccat cgaacatctt accgatgaaa agttcacata cgagaagccg
     4441 ctgcaatgag ttttatcgcg cacaacatgg caccgctgat gttcggcggc atgattctct
     4501 tcatgctgat cggctacccg gcggccttct cgctcgccgc caccggcttg tttttcggct
     4561 tcctcggcat tgaattgggc ctgatccggc ccgacttcct cggcaatttg acctatcagc
     4621 tgttcggcat catctccaac gacctgttgt tggcgatccc cttcttcacc ttcatgggcg
     4681 cgatcctgga gcgctgcggg ctcgccgaag acctgctcga ttccatcggc cagctgttcg
     4741 ggccggtgcg cggtggcatg tcctatgcgg tgatttttgt cggtgccatt ctcggcgcca
     4801 tcaccggcac ggttgcggcc tccgtgatcg ccatgggcgt catctccatg gtgcccatgc
     4861 tcaagtacgg ctactcgacg cgccacacca tgggggtgat cgcggcctcg ggcaccatca
     4921 cccagctcat cccgccctcc ttggtgctgg tggtgctggc cgacgtgctc ggccgctcgg
     4981 tcggcgacat gtacgccggc gccattggac caagctttat ccaagtcgcc ctgttctgtg
     5041 cttgggtcgc catcgtcagc gtgctgtggc caaaggacgt gccggccctg ccgctggagg
     5101 cgcggacgct cagtggctcg gcgctctggt tcaagtgctt gcgcggcatg atcccctcgc
     5161 tcggcctgat cttcctggtg ctcggcacca tctttatggg cttggcgacg ccaaccgagg
     5221 ccggcgccat gggcgtggtc ggcgccatcg cgcttgccgc atcctacgga actctgacct
     5281 ggaagttgct ctaccagggc atggccagca cgatgcgcct caccgccatg gtcgtctata
     5341 tcctgatcgg cgcccgcgtc ttcagcctgg tgttccaggg cgtcggcggc aaggaatgga
     5401 ttgagcactt gctcacctcg ctgcccggcg gccaggtcgg tttcctggtg tttgtcaacg
     5461 ccttcatttt catcgtcgcg ttcttcctcg atttcttcga gatcgccttc atcatcattc
     5521 ccctgctggc accggtggcc gacaagctcg gcatcgatct cgtttggttc ggcgtgctca
     5581 tctgcgccaa cattcagacg agcttcatgc acccgccctt cggctttgct ctcttctatt
     5641 tgcgcggcat cgccccgccc tcgatcaaaa cctccgacat ctactggggt gccatcccct
     5701 gggtgttcct gcaggtcatc ctcgtcatca tcgtgatctt ttggcctggc tcggtcacct
     5761 actggatcga gaagggcacg ggcgtcgatc cgtccaaggt cgagatcaat gtgccgatgc
     5821 cgaccctgcc gccggccttc gatccgtcgc aattcaagta gccggccagc gcggcgccag
     5881 ggtatgcggg gccgctcaca aggccagcca aggccccggt gggccggaaa ggggcgcgct
     5941 ttagcccacc atcgttcaat cacgattggg atatattagc tccgcacgcc gctagagccg
     6001 cggcgggtgc gggcgcatga ggacgaagat ggtcaaggtc atcgcctttt ggggcatcat
     6061 ctccattgtg tgcgccttca ttgccggtat cgtggccggc ctgaagcgcc gcgaccattc
     6121 cttctgggcc gcctggagct tcctgttccc gcccatgctc ctgctcttgc tcgtgatgcg
     6181 tcccaatcga ggcccgcggc cgcgacgccc cggtctggac gagctcgagg ccgacgatcg
     6241 ccgctttctc tgaaacgccc gcaggcggcg aagccgtttg ttaggcggcg ctcgcgcgct
     6301 tttgcctgat cgccgcctgc gcggcggcga gccgcgccac cgggacgcgg aagggcgaac
     6361 aggagacgta atcgagtttg ct
//
LOCUS       DASTMT010000514         2844 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_4227998, whole genome shotgun sequence.
ACCESSION   DASTMT010000514 DASTMT010000000
VERSION     DASTMT010000514.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2844)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2844)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2844
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4227998"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(179..304)
                     /locus_tag="VFR00_11840"
     CDS             complement(179..304)
                     /locus_tag="VFR00_11840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0160003.1"
                     /translation="MQSNHEDNYTKTDIAVAIVLAAIATLWIIAYIIIFVQSGPA"
     gene            468..953
                     /locus_tag="VFR00_11845"
     CDS             468..953
                     /locus_tag="VFR00_11845"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HEU0160004.1"
                     /translation="MLGRRLDWALSQVPRALKWLALPISLLLFLQWPLRDYVQAYSRE
                     ANDLGQWLFALYVAASVLAATRAGTHLATDAVARRYRPSTRRLIAHAGNLLALMPWAL
                     CILATATPTILRSLGQLERFPETGNPGYFLVKLALWVLAVGVLASAIVDLIKPDARDE
                     P"
     gene            953..2446
                     /locus_tag="VFR00_11850"
     CDS             953..2446
                     /locus_tag="VFR00_11850"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEU0160005.1"
                     /translation="MAGFGLALLACIGVTLILTGLPAYVVLILAAVLGAAAAVAGGSA
                     PLALLGTLSGRLINLFESDLLQALPLYVLMGALLNRLTLAPALFRTLLGLLPRQPGAA
                     VVSGLGLGALLAPMSGSVGASVLALARAAEPSLHAAGLPTPLRHATIAVASTLGVVIP
                     PSLVLILLGDAMLSAHTFAINATGRTDRVINTQDVLRAAIVPAALFLVLSLVAGWYSA
                     RRSRSEQPGAARLACAKPSDMVLTAATVTFLVLLLGGVAAGYIFAVEGAATGAVLLML
                     MGVLTRQLDRQTLGPMLTECMATTGALFAPLLAATSFTLVLRLLGTDKLIERWVTALP
                     GGEITATTAVLGAIFVAGFVLDAFEIIFVAVPILIPSLLIRVPDAVWVSTLVLLTLQA
                     SFLLPPIGYALMMTRGVLGSHVAPAAFARALLPYLAAQILVLFLTLALPRLVHVLEPA
                     NTHSRGQRTPPLGDDVEDRLRQMLPPPIPPGLPPIGAPAFPAPPGGE"
     gene            complement(2549..>2844)
                     /locus_tag="VFR00_11855"
     CDS             complement(2549..>2844)
                     /locus_tag="VFR00_11855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HEU0160006.1"
                     /translation="VCGPGEEGPQFTEADVARAQLIHDLQRDLGVNDEGIGVILDLID
                     QLHGMRRTLGQLLSAIQAQPDDVRESLVVSARQAILRHHAGPDRQPGGNQPGS"
BASE COUNT          406 a          973 c          902 g          563 t
ORIGIN      
        1 gcggcgtagg cgaatcgggc ttttttttgc gtcggcgcgg cgggcaagaa caaaaacgca
       61 acctggtcct cccgcgcggt cgctcggtgc gctcgaggag gggcgtcagg ccgcagccat
      121 ggccaccccc tcgggctggc cttcccggaa accaacgcag gctcggacaa gcgccctctc
      181 aggccgggcc gctttgcacg aaaatgataa tgtaggcgat gatccagagg gtggcgatcg
      241 ccgccaggac gatcgcaacg gcaatatccg tcttggtgta attgtcctcg tggttgctct
      301 gcatggcgcg gggtcgatca caagcggcca caaatcggct cgcgaaattg gcacgacccg
      361 gcgcgccgtg ccaacccccg ccgcgaccgc gaggtcgagg actttgtggc acgatcggac
      421 cggttgtata gtcgaggaga gccggtaaga gagcgggcta atccaaaatg cttggacgcc
      481 gactggattg ggcgctgagc caagtgcccc gcgctctcaa atggctggcc ctacccatca
      541 gcctgttgtt gttcttgcaa tggccgctca gggactacgt ccaagcctat tcgcgcgagg
      601 ccaatgacct ggggcaatgg ctatttgctc tctatgtcgc cgccagcgtc ctggccgcga
      661 cccgcgccgg cacacacctg gcaaccgatg ccgtcgcgcg tcgctatcgt cccagcacac
      721 ggcgccttat tgctcacgcg ggcaatctcc tggcgctgat gccctgggct ttgtgcattc
      781 tcgctaccgc cacccccacc attctgcgct cgctcggcca gctggagcgc ttccccgaaa
      841 ccggcaatcc cggctacttc ctggtcaaat tggccttgtg ggtattggcg gtgggcgttc
      901 ttgcgtccgc catcgtcgat ctgatcaagc cagatgccag ggacgagccg tgatggcggg
      961 gttcggtctc gccctcctgg cctgcatcgg cgtcacgctg attttgaccg gcctgcctgc
     1021 ctacgtcgtg ctcattctcg ccgccgtgct cggagccgcg gccgcggtcg ccggcggcag
     1081 tgccccgctc gcgcttcttg gcaccttgtc cggccggctc atcaatctgt ttgagagcga
     1141 tcttctgcag gcactgccgc tctatgttct gatgggcgca ctccttaatc ggctgacgct
     1201 ggcgcccgcc ctattccgca ccttgctcgg gctgctgccc cgccaacccg gcgcggcggt
     1261 ggtgtcgggt ctggggctag gcgccctgct ggcacccatg agcggctcgg tcggcgccag
     1321 cgttcttgcg ctggcgcggg ccgccgagcc aagtctgcat gcggcaggcc tgccgacacc
     1381 gctacgccac gccaccattg cagttgccag caccttaggc gtggtcatac cgccatccct
     1441 ggtcttgatc ctgctcggcg atgccatgct ctcggcccac acgttcgcca tcaacgccac
     1501 aggtcgcacg gaccgcgtta tcaatacgca ggacgtgctg cgggccgcca tcgttccggc
     1561 ggctctgttt ctcgttctca gcttagtcgc cggctggtat tcggcgcggc gcagccgtag
     1621 cgagcagccg ggcgcggcca ggctcgcgtg tgccaaaccg agcgatatgg ttttgaccgc
     1681 ggccaccgtg acctttctcg tgctgctgct gggtggcgtc gccgccggtt acatctttgc
     1741 ggtggagggt gccgccaccg gtgctgtgct gctgatgctg atgggcgtgc tgacccgtca
     1801 gctcgatcgc cagacgctcg ggccgatgct gacggaatgc atggccacca ccggcgcact
     1861 gttcgccccg ctgctggcgg ccaccagctt cacgttggtg ttgcgcctgc tcggcaccga
     1921 caagctgatc gagcgttggg taaccgcctt gccgggcgga gaaatcaccg ccaccaccgc
     1981 cgttctgggc gccattttcg tggccggatt cgtcctcgat gccttcgaga tcatcttcgt
     2041 ggccgtgccg atcctcatcc cgtcgctgtt gatacgggtg cccgatgcgg tctgggtttc
     2101 gacgcttgtg ctcctcacgc tgcaagcaag cttcctgctg ccaccgatcg gctatgcgct
     2161 gatgatgaca cgcggggtcc tcggctcgca cgttgcgccc gccgcctttg ccagggccct
     2221 gctgccgtac ttggcggcgc agatcctcgt gttgttcctc acgctcgcct tgccgcggct
     2281 cgtgcatgtg ctggagccgg cgaacacgca ctcgcgcggg caacggacgc cgccgctggg
     2341 cgacgatgtc gaggatcggt tgcggcaaat gctaccgcct cccattccac ccgggcttcc
     2401 acccattggc gccccggcgt ttccggcgcc tccgggcggc gagtaggctg ccaacagcag
     2461 ccgagctcga ccgctcgcgt ccaacgtcgt gtcgttgctg gggccaaggt cgtgccagca
     2521 gccggagggc cgcggccgcc acaccgcttc aggaccccgg ttggttgccg ccgggctgtc
     2581 gatcggggcc ggcgtggtgc cgcagaatgg cttgccgggc gctgacaacg aggctttcgc
     2641 ggacatcgtc tggttgcgct tgaatggccg acaagagctg gcccagcgtg cgacgcatcc
     2701 cgtgcagttg atcgataagg tcaagaatga cgccaatgcc ctcgtcattg acgccgaggt
     2761 cgcgctgcag atcatggatc aactgcgccc gcgcgacgtc agcctcggtg aattgtggtc
     2821 cttcctctcc cgggccacac acca
//
LOCUS       DASTMT010000515         2012 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_1934656, whole genome shotgun sequence.
ACCESSION   DASTMT010000515 DASTMT010000000
VERSION     DASTMT010000515.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2012)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2012)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2012
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1934656"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..585
                     /locus_tag="VFR00_11860"
     CDS             <1..585
                     /locus_tag="VFR00_11860"
                     /inference="COORDINATES: protein motif:HMM:NF014605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEU0160007.1"
                     /translation="IDFVTILGPAGTGKTLLTLAAGLAQTLESNRYSEIIMTRVTIPL
                     GEDIGFLPGTEEEKMEPWMGALMDNLEVLTQSQEGGNWGRAATNDLLRNRIKIRSLNF
                     MRGRTFLNRFLILDEAQNLTPKQMKALVTRAGPGTKLVCLGNIAQIDTPYLTETTSGL
                     TYVVNRFRGWAHSGHITLVRGERSRLADYASDIL"
     gene            585..1046
                     /gene="queF"
                     /locus_tag="VFR00_11865"
     CDS             585..1046
                     /gene="queF"
                     /locus_tag="VFR00_11865"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HEU0160008.1"
                     /translation="MPKRRETLTQLGQRVAWPESPDEAQLERVPNPHPDKVYLARFTA
                     PEFTSLCPVTGQPDFAHLVIDYVPRRWLVESKSLKLYLGSFRNHGAFHEDCTVSIGKR
                     LVALLEPHYFRIGGYWYPRGGMPIDVFWQAGTLPENLWLPDQGVAPYRGRG"
     gene            complement(1055..1858)
                     /locus_tag="VFR00_11870"
     CDS             complement(1055..1858)
                     /locus_tag="VFR00_11870"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018063325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEU0160009.1"
                     /translation="MEFKGKVALITGAGNGIGRAAALGFAARGAEVVAVDLDAAAAER
                     TAASVKQQGGNALALRADVTKSADVQAYVKATLDAHGRIDCFFNNAGIEGKVAPTAEY
                     DEAVFDQVINVNVKGVFLGLRHVLPVLLRQKAGAVVNTASVAGLVATPGMSAYVASKH
                     AVLGLTKAAAGEVARLGVRVNAVCPGPIDTRMIHSLESQLNPGNPASVGDRYRSSLPL
                     GRYGTAEEVANVVLFLCSDLAGNITGAHYVVDGGRTATGGSVTAAPKAE"
BASE COUNT          356 a          687 c          654 g          315 t
ORIGIN      
        1 atcgacttcg tgacgatcct cggtccggcg ggcactggca agacgctgct gacgctggcg
       61 gcaggcctgg cgcagacgct ggagagcaat cgctacagcg agatcatcat gacgcgcgtg
      121 acgatcccgc tcggcgagga catcggcttc ctgcccggca ccgaggaaga aaagatggag
      181 ccgtggatgg gcgcgctcat ggacaacctg gaagtgctga cgcagagcca ggaaggcggc
      241 aactggggcc gcgccgccac caacgatctg ctgcgcaacc ggatcaagat ccgctcgctg
      301 aatttcatgc gcggccgcac gttcctcaac cgctttctga ttctcgacga ggcgcagaac
      361 ctgacgccga agcagatgaa ggcgttggtc acgcgcgccg gcccgggcac caagctcgta
      421 tgcctcggca acatcgcgca gatcgacacg ccgtacctga cggagacgac ctcgggattg
      481 acgtacgtgg tcaatcgctt ccgcggctgg gcgcattcgg ggcacatcac gctggtgcgc
      541 ggcgagcgct cgcggctcgc ggactacgcg tcggatattc tgtaatgccc aaacgccggg
      601 aaacgctcac gcaacttggc cagcgcgtgg cctggccgga gtcgccagac gaggcgcagc
      661 tcgagcgcgt gccgaatccg catccggaca aggtgtacct ggcgcgtttc acggcgccgg
      721 aattcacctc gctgtgcccg gtcaccggcc agcccgactt cgcgcatctg gtgatcgact
      781 atgtgccgcg gcgctggctg gtcgagagca agtcgctgaa gctctatctc ggctctttcc
      841 gcaaccacgg tgccttccac gaggactgca cggtttcgat cggcaagcgc ctcgttgccc
      901 tgctcgagcc ccactacttc cgcatcggcg gctactggta tccgcgcggc ggcatgccga
      961 tcgacgtgtt ctggcaggcg ggcacgctgc cggaaaacct gtggctgccg gatcaggggg
     1021 tggcgcccta ccgcggccgc ggctgagcca agcctcactc cgccttcggc gccgcggtca
     1081 ccgagccgcc cgtggcggtg cggccaccgt cgacgacata gtgcgcaccg gtgatgttgc
     1141 ccgccagatc ggagcacaag aacagcacga cgttggccac ctcttcggcg gtgccgtagc
     1201 ggccgagggg aaggcttgac cggtagcggt cgccaacgct tgcgggattg ccgggattga
     1261 gctggctctc gagcgaatgg atcatgcgcg tgtcgatcgg accgggacaa actgcattga
     1321 cgcgcacgcc caagcgcgcc acctcgccgg cggcggcctt ggtcagaccc aacacggcgt
     1381 gcttggaggc gacataggcc gacataccgg gcgtagcgac caggcccgcc accgaggcgg
     1441 tgttcacaac cgcgccggcc ttctgccgca acagcaccgg cagcacgtgg cgcaggccca
     1501 agaacacacc tttgacgttg acgttgatga cctggtcgaa caccgcttcg tcgtattcgg
     1561 cggtgggggc gaccttgccc tcgatgccgg cgttgttgaa aaagcaatcg atgcgaccgt
     1621 gggcatcgag cgtcgccttg acgtaggcct gaacgtcggc cgatttggtt acgtccgccc
     1681 gcagagccag cgcgttgccg ccctgctgct tgacgctggc cgccgtgcgt tcggccgccg
     1741 ccgcatcgag gtcgacggcc accacctcgg caccacgcgc ggcaaagccg agggccgcgg
     1801 cccggccaat gccattgccg gcccccgtga tgagcgcgac cttgcccttg aactccatag
     1861 ctgctcctcc aaccctgcgc ctgaatttaa aaaaggatag ccaggcgcgc gcgccaaggc
     1921 cagcccgccc caaggccagc agcccaagcc cagaccggag gcccgacctt gccagcggcc
     1981 gcgaaggctg gaaagctcgc gcatcgcatc tt
//
LOCUS       DASTMT010000516         4494 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_1280576, whole genome shotgun sequence.
ACCESSION   DASTMT010000516 DASTMT010000000
VERSION     DASTMT010000516.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4494)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4494)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4494
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1280576"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..626
                     /locus_tag="VFR00_11875"
     CDS             <1..626
                     /locus_tag="VFR00_11875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012335883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HEU0160010.1"
                     /translation="LLTDGTMLEFLHSHSSVIYQPSFNDRRFGGFNPYALGFAMMSDI
                     KRMCLEPSEEDRAWFPDFAGSGDPLGTLRQAWAEFRDESFILQYLSPKLMRDFRLFAV
                     ADDAKDAAMRVAAIHDEQGYRDIRRRLARQYDIAVQDPDLQVTDADLSGSRRLVLTHH
                     VRNGILLDKAEAERTLQYLAQLWGYRVKLIESDDGRVLKEHEALPMP"
     gene            complement(642..1361)
                     /gene="pdeM"
                     /locus_tag="VFR00_11880"
     CDS             complement(642..1361)
                     /gene="pdeM"
                     /locus_tag="VFR00_11880"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ligase-associated DNA damage response
                     endonuclease PdeM"
                     /protein_id="HEU0160011.1"
                     /translation="MLELKSERLELLADTAGLNLSICGKALIADNSGAIYWPAEATLL
                     VADLHLEKASAYANAGALLPPYDTRETLIRLAQSIDRYQPRCVIALGDSFHDEGALER
                     IDADDLERLRILQEDRDWIWLSGNHDPRAGIGGRRADAIRLGGIKLRHQPTRGAATHE
                     IAGHMHPAARLSLYGTSIRRPCFVGNGRRIIMPAFGAFTGGLNVLDEAFVPLFGDGGM
                     AVWMLGQEGLYAVATRLLSRD"
     gene            complement(1363..3942)
                     /locus_tag="VFR00_11885"
     CDS             complement(1363..3942)
                     /locus_tag="VFR00_11885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012093375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ligase-associated DNA damage response DEXH box
                     helicase"
                     /protein_id="HEU0160012.1"
                     /translation="MNIGSQLHPEPISQWAAGQPAAPRAAAPLPPVFMDWFARRGWQP
                     RAHQLQLLANVNQGRSTLLIAPTGAGKTLAGFLPSLVALAQRAASPGAHSVHTLYVSP
                     LKALASDVARNLMAPVAEMGLSVSVETRTGDTPIARKQRQRQRPPELLLTTPEQVALL
                     LSHADAARLFAGLKTVIIDEIHALTANKRGDLLALGLARLLRLAPEQVRIGLSATVAR
                     PSELRAFLAPQTMAASIGLAHLITVEGGQPHTIEMLDSATPLPWAGHTSRYAVPDMLA
                     AIGAHRLSLLFVNTRNQAELLFQELWRRNEHNLPIALHHGSLEAAQRRKVEAAIAAGR
                     VRAVVSTSTLDLGVDWGNVDLVINVGAPKGASRLLQRVGRSNHRLEEASRALLVPSNR
                     FEVLECRAAIDAARAGTQDALARRPGALDVLAQHVWGMACSGPLQPISLYREVRSALP
                     YCDLPRRSFDRVLDFVATGGYALRSYERYARLRPDGAGLLRLAHPRLARQYRMNVGTI
                     VEAPMIKIHLSARRRSKSARGKALPGGRVLGEVEENFVEQLKVGDTFAFAGEVLRFAG
                     LKDTDAFVTRTSDPEPMIPSYQGGKFPLSTHLAERVRALLAEPRRWQVLPQAVCDWLL
                     RQQERSSLPALGEVLIESFPRAGRHYLVAYPFEGRLAHQTLGLLLTRRLDRIGARPLG
                     FVATDYALAIWCLADLGQLRRQGQLDLELLFAEEMLGDDLEAWLQDSNLMRRTFRLNA
                     VIAGLIERRHRGGTKSARQVTIASDLIYDVLRKHDPGHILLQAAWEDAATGLLDVPRL
                     GEFLRRIRGRIRYKALGQVSPLAVPVLLEIGQELVNAKGVAEGILREAADALLREAMQ
                     GGG"
     gene            4025..4318
                     /locus_tag="VFR00_11890"
     CDS             4025..4318
                     /locus_tag="VFR00_11890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013164909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6460 domain-containing protein"
                     /protein_id="HEU0160013.1"
                     /translation="MRKGSRMDRQQFFGDSVGRTLVRLVLLSIVVGIIFSVLGITPFN
                     LIDRLEQLIRRILDLGFDAFRSAFGYFLLGAVVVIPVWFIMRLLKVGRSSDRR"
     gene            complement(4336..>4494)
                     /locus_tag="VFR00_11895"
     CDS             complement(4336..>4494)
                     /locus_tag="VFR00_11895"
                     /inference="COORDINATES: protein motif:HMM:NF013869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MscL family protein"
                     /protein_id="HEU0160014.1"
                     /translation="LNALLKFVITALAIFLLIRAINRFKKPAVVEAPPPSPSEIYLRE
                     IRDALVKK"
BASE COUNT          769 a         1470 c         1480 g          775 t
ORIGIN      
        1 gtctgttgac cgacggcacc atgctggagt tcctgcactc gcattcctcg gtgatctatc
       61 agccctcctt caatgaccgt cgcttcggcg gcttcaaccc ctatgcgctc gggtttgcca
      121 tgatgagcga catcaagcgc atgtgcctgg agcccagcga ggaggaccgc gcctggtttc
      181 ccgatttcgc cgggtcgggc gaccccctcg gcactctgcg ccaagcttgg gccgagtttc
      241 gcgacgagag cttcatcctg cagtatctaa gccccaagct gatgcgcgat tttcgcctgt
      301 ttgccgtcgc cgacgatgcc aaggacgccg ccatgcgcgt ggcggccatc cacgatgagc
      361 aaggctacag ggacatccgc cgtcgcctgg cgcgccagta cgacattgcc gttcaggatc
      421 ccgatctgca ggtcacggat gcggatttgt ccggtagtcg gcgcctcgtg ctcacccacc
      481 acgtccgcaa cggcatcctg ctcgataagg ccgaggccga acgcaccctg caatacctgg
      541 cccagttatg gggctatcgg gtgaagttga tcgaaagcga cgacgggcgc gtcttgaagg
      601 agcacgaggc cctaccgatg ccctaggccc aggcgcgcgg ctcagtcgcg actgaggagg
      661 cgcgtggcaa cggcgtagag gccttcttgc cccagcatcc acacggccat gccgccgtcg
      721 ccgaacaagg gcacgaacgc ctcatcgagc acgttcagtc cgccggtgaa ggcgccaaag
      781 gcgggcatga tgatgcgccg gccattgccg acgaagcacg gtcggcgaat ggacgtgccg
      841 tagagcgaca gcctggcggc aggatgcatg tgccccgcaa tctcgtgtgt cgccgcgccc
      901 cgcgtcggct ggtgtcgcag cttaatgccg cccagcctga ttgcatcggc gcgcctaccg
      961 ccgatcccag cgcgcgggtc gtggttgccg ctgagccaaa tccagtcgcg atcctcctgc
     1021 aggatcctca agcgctcgag atcgtcggcg tcgatacgct ccagggcgcc ctcatcgtgg
     1081 aaactgtcgc cgagcgcgat cacgcagcgc ggctgataac ggtcgatgct ctgcgccagc
     1141 ctgatcagtg tctcgcgggt gtcgtaggga ggtagcaagg cacccgcgtt cgcgtaggcg
     1201 gaggccttct ccaagtgcag atccgcgacc agcaaggtcg cttccgctgg ccagtagatc
     1261 gcgcccgagt tgtcggcgat cagcgccttg ccgcaaatgg aaaggttaag gcccgccgtg
     1321 tccgccaaca gctcgaggcg ttctgacttg agctcgagca tgctacccac ccccttgcat
     1381 ggcctcccgc aggagcgcgt cggccgcctc gcgcaggatg ccctccgcca cgcctttggc
     1441 gttgaccaac tcctgtccaa tttccaacaa caccggcacg gccagcggcg acacctgccc
     1501 gagcgccttg tagcggatcc ttcccctgat tcgccgtaaa aactcgccca gccgaggcac
     1561 atccaagagg cccgtggcgg catcttccca ggccgcctgc aacaggatgt ggcctggatc
     1621 gtgcttgcgc agcacgtcgt agatcagatc cgaggcgatg gtgacctgcc gggcactttt
     1681 cgtgccgccg cgatggcggc gctcgatcag cccggcaatc accgcgttga ggcgaaaggt
     1741 gcgccgcatc aggttggagt cttgcaacca agcctcgagg tcgtcgccca gcatctcctc
     1801 ggcgaacagc agctcaaggt cgagctgacc ctggcggcgc aattggccga ggtcggccag
     1861 gcaccagatc gccagcgcat aatcggtggc gacaaagccc aggggtctgg cgccgatgcg
     1921 atcgaggcgc cttgtcaaaa gcagaccgag cgtctgatgc gccagcctgc cctcgaacgg
     1981 ataagccacc aaataatgcc gcccagcgcg cggaaaactt tcgatcagca cttcgccgag
     2041 cgctggcagc gaggatcgct cctgttggcg caaaagccaa tcgcataccg cctgcggtag
     2101 cacctgccag cgccgcggct cggccagcaa ggcgcggacc cgttcggcca aatgcgtgga
     2161 aagtggaaat ttgccgccct ggtaggaggg gatcatcggc tccggatcgc ttgtgcgcgt
     2221 cacgaaggca tcggtgtcct tcaaccctgc aaagcgcaaa acctcgccgg cgaaggcaaa
     2281 ggtgtccccg accttgagct gttcgacgaa attctcctcg acctcgccca agacgcgccc
     2341 gcccggcagg gccttccccc gagcggattt gctgcgccgc ctggccgaca ggtggatttt
     2401 tatcatcggt gcttcgacga tggtgccgac gttcatgcgg tactggcggg caaggcgtgg
     2461 gtgagccagg cgcaggaggc ccgcgccgtc cggccgcaag cgggcgtaac gttcgtagct
     2521 gcgcagcgca tagccgccgg tcgccacgaa gtcgagcacc cgatcaaaac tgcgccgggg
     2581 caaatcgcag tagggcaaag cgctgcgcac ttcgcggtaa aggctgatcg gctggagcgg
     2641 gccgctgcag gccatgcccc agacgtgttg cgccagcacg tcgagcgcac ccgggcgccg
     2701 cgccagggca tcctgcgtgc ccgcccgcgc cgcgtcgatg gcggcacgac actcaagcac
     2761 ctcgaagcga ttcgacggca ccaacagggc acgggaggct tcttccaggc ggtgattgga
     2821 gcggccgacg cgctgcagca atcggctcgc tcccttcggt gcgcccacat tgatgacgag
     2881 gtcgacgttg ccccagtcca cgccgagatc gagcgtggag gtcgacacca ccgcgcgtac
     2941 gcgaccagca gctatggccg cttcgacctt gcgccgctga gccgcctcga gggagccgtg
     3001 gtggagggcg atcggcaggt tgtgctcgtt gcggcgccac agctcctgga acagcagctc
     3061 cgcctggttg cgggtgttga cgaacagcaa cgacaaccga tgcgccccaa tggccgcgag
     3121 catgtccggc acggcgtagc ggctggtgtg cccggcccaa ggcagcggtg tggcgctgtc
     3181 gagcatctcg attgtgtgcg gttgaccgcc ctcgacggtg atcaggtgcg ccaggccgat
     3241 gctcgccgcc atggtctgcg gcgccagaaa ggcacgcagc tcactgggac gggcaaccgt
     3301 cgccgacaga ccgatacgca cctgctccgg cgcgaggcgt aaaaggcgcg cgagaccgag
     3361 tgccagcaag tcaccgcgct tattggcggt gagcgcatgg atttcatcga tgatgacggt
     3421 cttcagaccg gcgaacaagc gggcggcgtc cgcatgcgac agcagcaagg cgacctgttc
     3481 cggcgtggtc agcagcagct cgggcgggcg ctggcgttgg cgctgcttgc gtgcgatcgg
     3541 cgtatcgccg gtgcgcgttt cgacgctcac ggacaagccc atttccgcca ccggcgccat
     3601 caggttgcgg gccacgtcgc tcgccagcgc cttcaggggc gagacgtaga gggtgtgcac
     3661 ggagtgagcg ccaggtgatg cggcgcgctg cgccagcgcc accaggctcg gcaggaagcc
     3721 cgccagcgtc ttgccggcgc cggttggcgc gatcagcagc gtcgagcgtc cctggttgac
     3781 gttggccaac agttgcagct ggtgcgcgcg gggctgccag ccgcggcgtg cgaaccagtc
     3841 catgaatacg ggcggcagcg gcgccgccgc ccgcggtgcg gcaggctgcc ctgccgccca
     3901 ttggctgatc ggctctgggt gcaattggga tccgatgttc attgctccgc ggcgccaggc
     3961 ctgacgcctg ttatgcaact caagactatg ttaatcggga ggcaggcgcc gcggcgcaag
     4021 gagcatgcga aagggcagcc gcatggaccg gcagcagttc tttggcgaca gtgtgggccg
     4081 caccttggtc cgcttggtgc tgctctccat cgtggtcggc atcatttttt cggtgctcgg
     4141 catcacgccc ttcaacctga tcgaccgtct ggagcagttg atccggcgca ttctcgatct
     4201 gggcttcgat gcctttcgct cggccttcgg ctattttctg cttggcgccg tcgtggtcat
     4261 tccggtctgg ttcatcatgc gccttttgaa ggtcgggcgg tcgagcgatc ggcgttgaag
     4321 ggcggcgcac cgaggttatt ttttgacgag cgcgtcgcgg atttcccgca ggtagatctc
     4381 ggacggcgag ggaggcggcg cctctacgac cgccggcttc ttgaacctgt tgatggcgcg
     4441 gatcagcagg aagatcgcga gcgcagtgat cacaaacttg aggagcgcgt tgag
//
LOCUS       DASTMT010000517         2137 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_2916835, whole genome shotgun sequence.
ACCESSION   DASTMT010000517 DASTMT010000000
VERSION     DASTMT010000517.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2137
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2916835"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(200..979)
                     /locus_tag="VFR00_11900"
     CDS             complement(200..979)
                     /locus_tag="VFR00_11900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009465392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="short-chain dehydrogenase/reductase"
                     /protein_id="HEU0160015.1"
                     /translation="MDLGLDGKQVLITGGSKGIGLALAHAFAAEGATVVMVARDKAKL
                     ESAAAEVARRHNARATAHASDLSDEAQRRRLHEAFPDVDILVNNAGAIPGGGIADISM
                     DRWQAAWALKVFGYIHLCQLYVASMKARRSGTIINIIGIGGRAVRPDYICGAAGNGGL
                     IAFTNALGAETPSYNVRVFGINPAATMTERIVELSRQRAKSRFGDESRWQEALGADKL
                     PFGRIKTAEEVAALATMLAAPQVHYLSGTVIDMDGGGQWRQ"
     gene            complement(1006..1788)
                     /locus_tag="VFR00_11905"
     CDS             complement(1006..1788)
                     /locus_tag="VFR00_11905"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEU0160016.1"
                     /translation="MTEPDTSTIDINGYPTRVWRKGAGPKIGFLAGYGGLPRWIPFLD
                     RLAECRTVIAPSLPGFPGGDRGHSVLDSQLDWLLAVRDLIDRADLAGSDLVGSSVGGS
                     FAAEVAAVWPDKVKRLALIAPFGLFDEQTPATDPWAQKQDDIAGLMTAQPEIYKALKQ
                     MPPGANSIEWPIEQTRAAEAAARIFWPLGNTRLERRLPLITAPTLILWGAEDRLLPPS
                     YAALVAAAMRARTETRIIAGAGHLAELDKPDDVAAAILSFMA"
     gene            complement(1832..>2137)
                     /locus_tag="VFR00_11910"
     CDS             complement(1832..>2137)
                     /locus_tag="VFR00_11910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0160017.1"
                     /translation="LARDMNNIVANGYVIVGSPDEVAEQLHELATTLNVGQLMLMMQF
                     GNLSSEVTKYNTRLFAEKVMPKLKGLFADWENRWWPRPMQKRQRAMLPAFMPQAAAQ"
BASE COUNT          368 a          702 c          693 g          374 t
ORIGIN      
        1 catgatgacg catcttgcca tctcggagac cagcgctgcc ggcgaggcca ctacctggct
       61 cgaaccagtg agcgatgccg actacctcat ggcggcagcg aagccgacat gaggcaacgg
      121 ctgccccatg gccgcggcgc cacctcggtt ggcgctgcac cgccacggcc ccacgccgac
      181 cgcgggcttt gcagcgtcgt cattgccgcc actggccgcc gccgtccatg tcaatgacgg
      241 tgccggacaa ataatgcacc tggggcgcgg caagcatggt ggcaagcgcg gccacttcct
      301 cggcagtctt aatgcgtccg aacgggagct tgtcggcccc gagcgcctcc tgccagcgac
      361 tttcgtcgcc gaagcgcgac ttggcgcgct ggcgggacag ctcaacgatg cgctcggtca
      421 tggtggcggc gggattgatg ccaaacacgc gcacattata gctgggcgtc tcggcgccga
      481 gcgcgttggt gaaggcgatc agaccgccat tgcccgccgc gccgcagata tagtcaggcc
      541 gcacggcgcg cccccctata ccgatgatgt tgatgatggt gccggacctg cgcgccttca
      601 tgctggcaac gtagagttgg cagaggtgga tgtagccgaa caccttgagc gcccaggccg
      661 cctgccaacg gtccatactg atgtcggcaa tacctccgcc gggaatggca ccggcgttgt
      721 tgacgaggat atcgacgtcg ggaaacgctt cgtgcagccg ccggcgctgg gcctcgtccg
      781 acagatccga ggcgtgcgcc gtcgcgcggg cgttgtgcct gcgggcgacc tcagccgccg
      841 cgctctcgag ttttgccttg tcgcgcgcca ccatgacgac agtggcgcct tcggccgcaa
      901 aggcatgcgc cagcgccaag ccgatgcctt tcgagccgcc ggtgatcaga acttgtttgc
      961 catcgagacc aaggtccatg cgcatcatcc ttccagccgg gtgacttacg ccatgaagct
     1021 caggatcgcg gcggcgacgt cgtcgggctt gtcaagctcg gccagatgtc cggcgccggc
     1081 gatgatcctg gtctccgtcc tggcccgcat ggccgcagcg acaagagcgg catagctcgg
     1141 cggcagcaga cgatcctcgg cgccccacaa gatgagtgtc ggggccgtga tcagcggcag
     1201 acggcgttcg agccgggtgt tgccgagtgg ccagaaaatt cgcgccgcgg cttcggccgc
     1261 ccgtgtctgc tcgatcggcc attcgatcga gttggcgcct ggtggcatct gcttcaaggc
     1321 cttgtagatc tcgggctgcg ccgtcatcag accggcaata tcgtcctgct tttgtgccca
     1381 agggtcggtg gccggcgtct gctcatcgaa caagccaaag ggtgcgatga gcgccaatcg
     1441 cttgaccttg tccggccaca ccgccgccac ctcggcggcg aacgagccgc cgaccgagct
     1501 accgaccaga tcgctgccgg cgagatcagc cctatcgata agatcgcgta ccgccagcag
     1561 ccagtcgagc tgcgaatcga gcacgctgtg cccgcgatcg ccgcccggga agcctggcaa
     1621 cgagggcgca atcaccgtgc ggcattcggc caagcgatcg agaaacggga tccatctggg
     1681 cagcccgccg tagccagcaa ggaaaccaat cttcggcccg gcgcccttgc gccagacccg
     1741 cgtgggatag ccgttgatgt cgatggtcga ggtgtcaggc tcggtcacgc tacccgctcc
     1801 ggctggcgag gggtggagga ttgagcaagc ttcactgcgc cgcggcctgc ggcatgaacg
     1861 ccggcagcat ggcgcgctgc cgcttctgca tcggccgtgg ccaccagcgg ttttcccaat
     1921 cggcgaacaa tcccttgagc ttcggcatca ctttctcggc gaacaggcgc gtgttgtact
     1981 tggtcacctc gctcgacagg ttaccgaact gcatcatcag catgagctgc ccgacgttga
     2041 gggtcgtggc gagttcgtgc agctgttcgg cgacttcgtc gggtgagccg acaatgacat
     2101 aaccattggc gacaatgttg ttcatgtcac gggcgag
//
LOCUS       DASTMT010000518         1835 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_5726201, whole genome shotgun sequence.
ACCESSION   DASTMT010000518 DASTMT010000000
VERSION     DASTMT010000518.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 1835)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1835)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1835
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5726201"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..817)
                     /locus_tag="VFR00_11915"
     CDS             complement(<1..817)
                     /locus_tag="VFR00_11915"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0160018.1"
                     /translation="MSAQAPQIADTPRLLPVMAWATASVFFFYAWIMRVAPSVMVEEL
                     MREFAVGAGVLGHLSAAYFYGYAGMQIPVGLLLDRFGPRRLITAAALCCAGGCVLFAA
                     GTTLATVAAGRFLIGAGAAFSLVGSMVVAGQWFSPPRFALLSGLAMAMGMAGGVFGQA
                     PLRVVVEATDWRTTSLALALGGVAIAFSAWLSVRDRWRGAGGVHNLFANLGLVLKHRQ
                     TWLVAVAGLGTSGPLLGFAGLWGVPFLQMAYGLERRDAAALTSLLFVGWGVGAP"
     gene            1295..>1835
                     /locus_tag="VFR00_11920"
     CDS             1295..>1835
                     /locus_tag="VFR00_11920"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012113349.1"
                     /note="catalyzes the formation of 2-isopropylmalate from
                     acetyl-CoA and 2-oxoisovalerate in leucine biosynthesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HEU0160019.1"
                     /translation="MSQQQPNRDHATVGRGSQQIANTDRVFIFDTTLRDGEQCPGATM
                     TFEEKLEVADFLEAMGVDIIEAGFPIASEGDFYAVSEIAKRIKKSVVCGLSRAGELDI
                     DRAGEAVKHAARPRIHTFISTSPVHRKHKLQKSEEQVLEMVQFQVTRARNWCEDVEWS
                     AEDATRTEIDYLCRCVQTAI"
BASE COUNT          385 a          588 c          607 g          255 t
ORIGIN      
        1 ggggcgcacc gacgccccaa cccacgaaca gcagcgaggt gagggccgcc gcatccctgc
       61 gctccaagcc gtaggccatt tgcaggaacg gtacgcccca caagccggca aagccgagca
      121 aggggcctga cgtgccaagg ccggcgacgg cgacgagcca ggtctgacga tgcttcagaa
      181 cgagccccaa attggcaaaa aggttgtgca cgcctcccgc accgcgccag cggtcgcgca
      241 ccgatagcca agccgaaaag gcgatcgcaa cgccgccaag ggccagcgcg agactggtgg
      301 tgcgccaatc ggtggcctcc accacaacgc gcaggggcgc ttgcccgaac accccaccgg
      361 ccatgcccat cgccatggcg agaccggaga gcaaggcgaa ccggggcggg gaaaaccact
      421 gaccggcaac gaccatactg ccgaccagag agaaggcggc gccggcgccg atcaggaaac
      481 ggccggctgc cacggtcgcg agcgtcgtgc ccgccgcaaa cagcacacac ccgcccgcgc
      541 agcacagagc cgcagcggtg atgagccggc gtgggccgaa gcgatcgagc aaaaggccga
      601 ccgggatctg cataccggcg taaccataga agtaggcggc cgacaagtga ccgagcaccc
      661 cggcgccgac ggcgaattcg cgcatcagct cctcgaccat gacgctcggg gcgacgcgca
      721 tgatccaagc gtaaaagaag aagaccgaag cggtcgccca ggccatgacg ggcaggagcc
      781 gcggcgtgtc agcgatctgg ggggcttggg cggacatcgg atcaagttcg acgaggagct
      841 ggaaaccgat acatacccga ggtggccggc caaatgagag cgaggaaagc gcaggccagc
      901 taggcgttgc acctgcctcc ctcatgcggc cggcctggcc ggtcccgagc gggagctcgc
      961 ttgggcgtgc ggggggcaaa aaaggcgagg gcgttggtgt tgttgccgat tgtgcttgcc
     1021 agacgccggg caatttggct taagtggccc ggcttgatac cggcaggtag gccttccgtg
     1081 agccagtcgc gctccagtgc tgcagcccca atgacggatg ggacgtttcc cgccgcgacg
     1141 ctgcttggcc tgctgaccct cctccttatg ggcccctgag ccaccgaggt ctggccttat
     1201 cgccggacgg gagctcaggg gatcgacggt tagccccaat cctctttctc gcctgcgcct
     1261 gtgcgcgcgg cacgacaagc aagggaatac gcctatgtct cagcagcaac caaaccggga
     1321 tcatgccacg gttgggcgcg gcagccagca aatcgccaac accgaccgcg tctttatttt
     1381 cgatacaaca ctgcgcgacg gcgagcagtg tcccggcgcg acaatgacct tcgaggaaaa
     1441 gctggaggtt gccgatttcc tcgaggccat gggagtggac attatcgagg ccggcttccc
     1501 cattgcctcg gaaggggact tttacgccgt ctccgagatc gccaagcgca tcaagaagtc
     1561 ggtggtttgc gggctgtccc gcgccggcga actcgacatt gatcgggcgg gagaagccgt
     1621 caaacatgcg gcgcggcccc gcatccacac cttcatctcc acgtctcccg tgcatcgcaa
     1681 gcacaagctg cagaagagcg aggagcaggt gctggagatg gtgcagttcc aggtgacgcg
     1741 cgcgcgcaat tggtgtgagg atgtcgagtg gagtgcggaa gacgccacgc gcaccgagat
     1801 cgattacctc tgccgttgcg tgcaaacggc gatca
//
LOCUS       DASTMT010000519        11613 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_3575826, whole genome shotgun sequence.
ACCESSION   DASTMT010000519 DASTMT010000000
VERSION     DASTMT010000519.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 11613)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11613)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11613
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3575826"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            163..3315
                     /locus_tag="VFR00_11925"
     CDS             163..3315
                     /locus_tag="VFR00_11925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEU0160020.1"
                     /translation="MIAKFFIERPILANVIALLTIILGLVALLRLPVAQYPNIVPPTV
                     QVTARYPGASAATVVRDVALPIEQKVNGVEGMIYMSSMSTSDGSYTLTVTFRIGTDAD
                     KAQILVQNRVTSAQPSLPQAVQVQGVQTAKRSTAILEIVALSSPDGRYDSLFLSNYAT
                     INLIDEIARVDGVGNVSVFGAGQYAMRLWLDPQKLQVRNLVPQDVINAVNLQSRATGA
                     GQLGIPPVPKGQEFQYTINVPSKLETVSQFEDIIIKADTSGSGRITRLRDVARIELGA
                     QQYSQTFKLDGQPAAGLAIFQLPEANALDVAKRVERRMQALEGAFPPGLTWTVPFDTT
                     KFVNAAIHEVYKTLIEAALLVLVVILVFLQDWRATLVPATTVPVTIIGAFAAMAAFGF
                     SVNLTTLFAIILAIGIVVDDAIVVVEGAAKHIEHGLTPHDATVKAMHELIGPIIGITL
                     VLMAVFVPAAFLPGLSGQMYRQFALVIAATAAISAINAMTLKPAQCALWLRQTRAPQD
                     RNLFYRGFNRVYAAAEGRYARLIAGMVSRSAGMAGIVAALVALAIYGLSRVPTAFLPI
                     EDQGYFLIAVQLPEGAALERTTRALDDVTTRVHAQPGVDKVVAIAGLAALNDNASLAN
                     AGIAYVILKDWSERGKGEDLLGLYYGLSERVKTLEDGTALVIPPPAIQGIGNVSGATM
                     KVELRDGSFDYAQLEHLAQAIVERASAQSIFQVARNSFRADAPQLNIVIDRVKSETLG
                     VPLGNALDTLSSFVGSSYITQFNKFGRVFQIYVQAEASARIQPEALRTLAVRNTAGGM
                     VPLGTLAEISNVSGPSLISLYNLYPAATVIANTARGFSSGQGLDVLGQIARETLPPTS
                     GFEWTAMSYQEKEIGNQIYVVYALSLLLVYMVLAGQYESWIAPLTVIVSVPLALLGTA
                     AALLALGAPNNLYTQIGLILLIALSAKNAILIVEFARALRRDEGYPIEKAAVLAARSR
                     FRPILMTSFAFILGVVPLVLGSGAGANAQKSIGIAVLTGMLGSTLLTVAFVPSMFVLL
                     QRFEEWRSARAKPKAELPAA"
     gene            complement(3430..4452)
                     /locus_tag="VFR00_11930"
     CDS             complement(3430..4452)
                     /locus_tag="VFR00_11930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018453047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ionic transporter y4hA"
                     /protein_id="HEU0160021.1"
                     /translation="MMGLMAIAGANGITAIAAGAVLIATVFAAVHHAEVVAHRIGEPF
                     GTLVLAVAVTVIEVALIVSIMLGFAADKAGLARDTVFAAVMIVCNGIVGLCLLAGGMR
                     HHEQGFQLQGASAALAVLAALTVLTLVVPNFTTSTPGPSFSNSQLVFAGLISLVLYGS
                     FVFVQTVRHRDYFLVLEGGDDDAHAPPPSNAVALISAVLLAVALIGIVGLAKSLTPAL
                     EAIISWLGAPKAVVGVAIALLVLLPEGLAAVRAARANRLQTSLNLALGSALASIGLTI
                     PTVAVVSMLLGQPLALGLGLKDMVLLALSLLVGVITLGTGRTTVLQGIVHLVIFATFL
                     FLAVVP"
     gene            complement(4651..5493)
                     /locus_tag="VFR00_11935"
     CDS             complement(4651..5493)
                     /locus_tag="VFR00_11935"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEU0160022.1"
                     /translation="MLYVPPSAWLLSFALTLLPVASSAQELTPVAAPKLEAAATPADP
                     LSTKATPAFGKQLPRVGRALRETRTAKVLAIGSSSTAGIGASSPTRTYTARLEVDLEQ
                     ALTGTDFDVVSHGLGGEVAQGAADRMKREVEEVKPDLVIWQVGTNDAFRHVAIDSFKI
                     CLRKTLAWLKEQQVDVILINPQYGDQLIKDAYYEDMVNAVAEVARDADVLLVDRFQLM
                     KKLQREHTDRLYLSADNLHMNDEGYRCLAEELTATIIKALPQPTHTTASVVTPAPGAG
                     LSLP"
     gene            6284..9382
                     /locus_tag="VFR00_11940"
     CDS             6284..9382
                     /locus_tag="VFR00_11940"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU0160023.1"
                     /translation="MARSCAACGSRVAADANFCSDCGARLAPQRQGPAPAEPGAERRQ
                     LTVMFCDMVGSTALSTRLDPEEQRDVVSRFQECCASQIERFDGNVAQFLGDGVLAYFG
                     YPTAHEDDAERALRAGLALIKALEKDEPSQACALSARIGIATGVVVVGDLERGGTRQE
                     NAAIGETTNLAARLQALAQPNGLLLCPETYRLAGLLFEYEDLELQALKGFAQPIHVRR
                     VIATSGIENRFEARHRAALSPLLGRDEELQLLLRRWAQAERADGRVVLLSGEAGIGKS
                     RLIRALEQRLIGASHRAFNYHASPHQQDSALQPIIQQLRRAASIEREDASERQLDKLD
                     AFIGACGGKVAADLPVFAALLSIPAHGRYALPDLPPHRLKERFLQALLQLWGELARDK
                     PLLVVFEDAHWLDPTSLELLSRAVDASLQWRLLLLVTARPEFSAPWPNHQHVSSVALS
                     RLGQSEGKALAQGLVEKALPEAVLAEIVRRTDGVPLFIEELTKTILETGVLREGEVRY
                     ELDGPLPALAIPATLHASLLARLDRLHSVKDVVESGAAIGREFSWDLVAAVSSLSQHD
                     LQLALEKLVTAGLIFQRGVQPNATFLFKHALVRDAAYAGLLRGRRAHVHGQIAKALEG
                     RPEVVASEPELLAHHLTEAGLWEAALPYWLQAAERAVARSALREAAKHFREAIRLSQR
                     LQPSLQRTRSELDVHIRLGPVVMATHGYSAPESLEIYRRADELVAEVGDVVERMDVLL
                     GLFNVHYGRCELQHARAIADEHMGLVERHASNLGRGHTLLGQHYLAIGAFVEASRHFQ
                     AALAIFAKTPEDPSVLGAFGSQQVVCLSLMAGIHFARGEMDLAQQASARSIELARSSR
                     HTMSTALAIVTDLLTPLPGGLDPDPNKAQEVVEFCREHGLRNFEAWATFAAGAVAARR
                     GEPAKGIAIMQAAMATAEGMGSRLLRPVQLATLAMAHAKLGAIEDCLRLNEEAIATAE
                     RSGEMQAAASLLRAYGEALLHLGRSDEARRQFERGFKLATAQQATFEAGRLKARLERL
                     AP"
     gene            complement(9482..10891)
                     /locus_tag="VFR00_11945"
     CDS             complement(9482..10891)
                     /locus_tag="VFR00_11945"
                     /inference="COORDINATES: protein motif:HMM:NF021508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2235 domain-containing protein"
                     /protein_id="HEU0160024.1"
                     /translation="MKRIVVCYDGTWNALTDPSAVTNVVRVGQAVRAADEQGRSQIVY
                     YNAGVGSGGPIDRFLGGVFGAGVRANVKRGLAFLTLNWAEGDEIYIFGFSRGAYTARA
                     LAGVITAIRGVPRQEYFHRLEEIWNFYRLSPRERYSDEARHKYEIDKMVAEVPRPAPL
                     IKCLAVWDTVGSYGIPAGLGLSGLARKLTSWTRGFHDNMLNSRIEVGLQALAIDELRP
                     AFPPTTWVGDPNEPNAHQHVEQVWFAGVHANVGGGYLRSGLSDLALIWIMTRVEALTK
                     LRFSPDYIAKNFWPCAACSLYRSSRGWWWFSSLVPYRRPLFPEPTPIEAWFKGKKEKR
                     NMIPINEKIHWSVIERLGRAAFVDEKLAKSYAPSNLPLALKPKAWREEERAALEQDPR
                     VAQMTPQEEALIKACRNPTLNPRFQNCALFCTLDDSHLQALKPWSLAALRSTFSAADR
                     RIRRLVGLRTDWNMHAPPH"
     gene            complement(11211..>11613)
                     /locus_tag="VFR00_11950"
     CDS             complement(11211..>11613)
                     /locus_tag="VFR00_11950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012563383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEU0160025.1"
                     /translation="RATLRARTPAQRLRAAALTFFRYYDDNPRDLDLGFYLFRGGMKP
                     QGLGKERDETLNAALCAALAPIADAALALGASGDDAKVLMVETFAHAAGLLLLAHTGR
                     MRTFGTSAPHLMERFVADRIAALTGTSKPSR"
BASE COUNT         1994 a         3731 c         3846 g         2042 t
ORIGIN      
        1 cgacggcggc ctacgtgtca tcaagagcgg tcttggcacc gaagatcgtg tcgtcgtcag
       61 cggcatccaa cgggccattc ccggcggcag cgtcaagccg atcgatgcgg cggcgacggc
      121 cgctgccgct cccgcggtgc cagcggcggg caaggacaag ccatgattgc caagttcttc
      181 atcgagcggc cgatccttgc caacgtcatc gcgctgctga ccatcatttt gggcctggtg
      241 gcgctgctgc ggttgccggt ggcgcagtat cccaacatcg tgccaccgac ggtgcaggtc
      301 accgcccgct atcccggcgc cagtgccgca accgtcgtgc gcgacgtcgc cctgccgatc
      361 gagcagaagg tcaacggtgt cgagggcatg atctacatgt catcgatgag caccagcgac
      421 ggctcctaca cgctgaccgt gacgtttcgg atcggcaccg atgccgacaa ggcgcaaatc
      481 ctggtgcaga accgggtgac gagcgcgcag ccgtcgcttc cgcaagccgt gcaggtgcag
      541 ggggtgcaga ccgcaaagcg ctccacggcg atccttgaaa tcgtggctct cagttcgccc
      601 gacggccgct atgacagcct gtttctgtcg aactatgcca ccatcaacct tatcgacgag
      661 atcgcgcgcg tcgatggcgt cggcaatgtg tcggtgtttg gcgccggcca atacgccatg
      721 cggctgtggc tcgacccgca gaagctgcaa gtgcgcaacc ttgtgccgca agacgtcatc
      781 aatgccgtca acctgcaaag ccgcgccacc ggggccgggc agctcggcat cccgccggtg
      841 ccgaagggcc aggaattcca gtacaccatc aacgtcccga gcaaactcga gactgtctcg
      901 cagtttgagg acatcatcat caaggccgat acctccggca gcggtcgcat cacgcggctg
      961 cgcgacgtgg ccaggatcga actgggtgcg cagcagtata gccagacctt caagctcgat
     1021 gggcagccgg cggccggcct cgccatcttc cagctccccg aggccaacgc ccttgatgtt
     1081 gccaagcgcg tcgagcgccg catgcaggcg ctcgaggggg cgtttccgcc gggcctgacc
     1141 tggaccgtgc ccttcgacac cacgaaattc gtgaacgcgg ccatccacga ggtctacaag
     1201 accttgatcg aagccgccct cctggtgctg gtggtgattc tcgtcttcct gcaggattgg
     1261 cgcgcgaccc tggttcccgc gaccaccgtt cccgttacca tcatcggggc gttcgcggcc
     1321 atggcggcgt tcggcttttc cgtcaatctg acgaccttgt ttgccatcat tctcgccatt
     1381 ggcatcgtcg tcgacgatgc catcgtggtg gtcgaaggcg ccgccaaaca catcgagcat
     1441 gggctaaccc cgcacgacgc gacggtgaaa gccatgcatg agctgatcgg tcccatcatc
     1501 ggcatcacct tggtgctgat ggcggtgttc gtgcccgcgg ccttcctgcc gggcctcagt
     1561 gggcagatgt accgtcaatt tgccctggtt attgccgcga ccgcggcgat cagcgccatc
     1621 aacgccatga cgctcaagcc ggcgcagtgc gcgttatggt tgcgccaaac gcgggcgccg
     1681 caggaccgca atctcttcta tcgcggcttc aatcgcgtct acgcggccgc ggaggggcgt
     1741 tatgcgcgac tgatcgccgg catggtgagc cgcagcgctg ggatggccgg gatcgttgcc
     1801 gccctggttg ccttggcgat ttacgggctg agccgtgtgc caaccgcctt tttgccgatc
     1861 gaggaccaag gctatttttt gattgcggtg cagctgccgg aaggcgcggc gctcgagcgc
     1921 acgacgcgtg ctctcgacga tgtcacgacc cgcgtgcacg cgcagcccgg tgtggacaag
     1981 gtggtcgcca tcgccggtct ggcggcgctc aacgacaatg ccagcctcgc caacgccggc
     2041 attgcctacg tcatcctcaa ggattggtcc gagcgtggca agggcgagga cctgcttggc
     2101 ctctactacg ggctctccga gcgggtgaag acgctggagg acggcacggc gctggtcatc
     2161 ccaccgccgg ccatccaagg catcggcaat gtctcgggcg ccaccatgaa ggtcgagttg
     2221 cgtgacggca gcttcgacta tgcgcagctc gagcacctcg cccaggccat tgtcgaacgc
     2281 gcctcggcgc agtcgatttt tcaggttgcc cgcaactcgt ttcgtgccga cgcaccccag
     2341 ctcaacatcg tgatcgaccg cgtcaagtcc gagacgctcg gcgtgccgct cggcaatgcg
     2401 ctggatacgc tgagttcctt cgtgggatcg agctatatca cgcagttcaa taagtttggc
     2461 cgcgtctttc agatctacgt gcaggccgaa gcctctgccc gcattcaacc cgaggccttg
     2521 cgcacgctgg cggtgcgcaa cacggcgggt ggcatggtgc cactcggcac gctggcggag
     2581 atcagcaacg tcagtgggcc ctcgctgatc tcgctctaca atctctatcc ggcggcaacg
     2641 gtgatcgcca acacggcgcg cggcttcagt tccgggcaag gcttggatgt gctcgggcag
     2701 atcgcccgag aaaccttgcc tcccaccagc ggcttcgagt ggacggcaat gtcctaccag
     2761 gaaaaggaga tcggcaatca gatctacgtc gtctacgcct tgagcctgct gctggtctat
     2821 atggttcttg ccgggcagta cgagagctgg attgcgccgc tgacggtgat cgtctccgtg
     2881 ccgctggcgc tgctgggtac ggccgccgcg ctgttggcgc tcggcgcacc caacaatctc
     2941 tacacccaga tcggtctgat tttgctgatc gcgctctccg ccaagaatgc cattctgatc
     3001 gtcgagttcg cacgcgcgct gcgccgggac gagggctatc cgatcgagaa ggcggccgtg
     3061 ctggcggccc gcagccgctt ccgtcccatc ctgatgacgt cgttcgcctt tattctcggc
     3121 gtcgtgccac tcgtgctggg ctcgggcgct ggcgccaacg cgcagaagtc gatcggtatt
     3181 gccgtcttga cgggaatgct cggctcaacc ttgctcaccg tcgcctttgt gccgtccatg
     3241 ttcgtcttgc tgcagcgctt cgaggagtgg cggtccgcgc gggcaaaacc aaaagccgag
     3301 ctgccagccg cctgagccgc ggtgagaggc gggccagacg ccgcgggcag gtatcggggg
     3361 ggcctcgaga cggccaagcg cgggctcgag gcgccgagcc tgctgcgcgg gagcgcctcg
     3421 acgccgcgct tagggcacca cggccaggaa caaaaacgtt gcgaagatca ccaaatgcac
     3481 gatgccctgc agcaccgtgg ttcgtcccgt acccagggtg atgacgccga ccagcaggct
     3541 cagcgccagc agcaccatgt cctttaggcc gaggccgaga gccagcggct ggccgagcag
     3601 cattgaaacc acggccacgg tcgggatggt cagcccgatg ctggcgagcg ccgagcccag
     3661 cgccaggttc aaactggttt gcagacggtt ggccctggct gcgcgcaccg ccgccaaacc
     3721 ttcgggcagc agtaccagca aggcgatggc gacaccgacc accgccttgg gcgcgccgag
     3781 ccagctgatg atggcctcca atgccggggt gagggacttg gctagtccga caatgccgat
     3841 cagggcgacg gccagcagca ccgcactgat gagggcgacg gcgttggagg ggggcggcgc
     3901 atgcgcatcg tcgtcgccac cctccagcac caggaagtaa tcgcggtgcc gcacggtctg
     3961 cacgaacacg aacgagccat agagcaccag cgagatcaag ccagcaaaga ccagctggga
     4021 attgctgaag ctgggaccgg gggtgctggt ggtaaaattc ggcaccacga gggtgagaac
     4081 ggtaagcgcc gcgagcacgg cgagcgccgc gctggcgccc tgcagctgaa aaccctgctc
     4141 gtgatggcgc atgccgccgg caagcaggca gaggccgacg atgccgttgc acacgatcat
     4201 gaccgcagca aacaccgtgt cgcgtgcgag ccccgcctta tcggcggcaa agccaagcat
     4261 gatggagacg atgagcgcca cttcgatcac ggtcaccgcc acggcgagca caagcgtacc
     4321 gaacggctcg ccgatccggt gggcgaccac ctcggcgtgg tgcacggccg cgaatacggt
     4381 ggcgatcaga accgcgcccg ccgcgatcgc cgtgatgccg ttggcaccgg caatggccat
     4441 gagacccatc atgacccaag ccgcggcggg ccacgcccaa gaccaccaag gcgtccctgt
     4501 tgcagtcgac atgccaaggc accccttcgg ttgcgcagga gccgaaggct acacgagccc
     4561 cagccccaag cgaaggcgcg ttgagtgcgg atgcgctaga acgccaacga aaggcattga
     4621 ggcgcgcaga aggggattgg gcgggaaccg ctagggcagc gagagaccag ccccaggcgc
     4681 cggtgtcacc acgctggccg tggtgtgggt tggctgcggc aaagccttga tgatggtcgc
     4741 cgtcagctct tcggcgaggc aacggtagcc ctcgtcgttc atatgcaggt tgtccgccga
     4801 cagatacaag cgatcggtgt gttcgcgctg cagcttcttc atcagctgga aacggtcgac
     4861 cagcagcacg tcagcgtcgc gggcgacctc ggcaacggcg ttgaccatgt cctcatagta
     4921 ggcatccttg atcaactgat cgccgtactg tggattgata aggatgacat cgacctgttg
     4981 ctctttgagc caggcaaggg ttttgcgcaa acaaatctta aagctgtcga tggcgacgtg
     5041 gcggaaggca tcattggtcc cgacctgcca aatgacgagg tcgggtttga cctcttccac
     5101 ttcgcgcttc atgcgatcgg cggcgccttg tgccacctcg ccgccgaggc cgtggctgac
     5161 gacgtcgaag tcggtgcccg tcagcgcctg ttccaagtcc acttccaggc gcgccgtgta
     5221 ggtgcgcgtt ggcgaggagg cgccgatccc ggcggtcgag gatgagccga tggccaagac
     5281 tttggcggtt cgcgtctcgc gcagggcgcg cccgacgcgg ggcaactgct tgccaaaagc
     5341 cggcgtggcc ttcgtagaca atggatcggc cggtgtggct gcggcctcga gcttgggcgc
     5401 agcaacgggg gtcagctcct gggcggagct tgccacggga agaagcgtga gcgcgaagct
     5461 cagaagccag gctgagggcg gcacgtacaa cacgtcgtat cccctccttt ccctatttgt
     5521 accccatatc tgcgatcgcg tccggcccct tcgcagctgg gattaatgag gttgaatggc
     5581 gaatgacctg gtgcgttcca cgccgctccg ctgagacttt tgcgccaggg gacgccacac
     5641 cccgtggagc gctcagcaca cggtttaacg cacggccgcg cagcggccga gacgaatggg
     5701 gcggcgcccc caaagtcgtt aaccaggcct cctgcatgtc tcgcctgcgg tttggacctc
     5761 tttgtcagcg ccagccgcat ggctcggggc tccgcatcag gagatggcgc cctttggctg
     5821 gttcggcatc cccgctttga gggagaaccg cggcgcgatc ccgccaactc ccaacgccac
     5881 gatagccctc agcccacgac ccttagccca cgatgcatgg gctcacgctt gggctgcgct
     5941 gcacggtatc ttggggcccc gtgtggacga ttcgccccct tcggttaagg ccttgtacag
     6001 gcctcgcgca aaactgtgaa gggattgcag ggaggacgcg aggccgcacg ctaggcgagc
     6061 cgtcccgcgc ggtcattttg gtgaccgttg gcgctgcttt cggcgcggcg gtgccgcccc
     6121 aagacgtctc tgcctccgtc tcatacttaa ggatgagacg gccctcggca acgtgctagg
     6181 ctggttgcgc gatcaagcat ggggtcgccc gaccgtggcg tgcgcaaggt gcggggcaag
     6241 tctagcggca ggcgccaagt tctgcgcggc gtgcggcagc gccgtggcgc gcagctgtgc
     6301 tgcctgcggt tcgcgcgtcg ccgcagatgc caatttttgc tccgactgcg gcgcacgcct
     6361 cgcgccgcag cggcaagggc ccgcgcctgc cgagccgggt gccgaacggc gccagctgac
     6421 cgtcatgttc tgcgatatgg ttggctcaac cgcgctctcg acccgccttg atccggaaga
     6481 gcagcgcgac gtcgtgagca ggtttcagga gtgttgcgcc agccagatcg agcggttcga
     6541 cggcaacgtc gcccaatttc tgggtgacgg cgtccttgcc tacttcggct atcccaccgc
     6601 gcatgaagac gatgcggaac gtgcgctacg cgccggcctg gcgctcatca aggcactcga
     6661 gaaggatgaa ccgtcgcagg catgtgcgct gagcgcccgt atcggtatcg ccacgggcgt
     6721 ggtggtggtc ggggacctcg aacgcggggg gacgcggcag gagaatgctg ccatcggcga
     6781 gacgaccaat ctcgccgcac ggctgcaggc gttggcccaa cccaacggcc tgctgctctg
     6841 cccagaaacc taccgcctcg ccggcctgct gttcgagtat gaggatctcg aactgcaggc
     6901 gctcaagggg ttcgcgcagc cgatccatgt gcgtcgggtt atcgccacga gcggcatcga
     6961 aaatcgcttc gaggcgcgcc accgcgcggc actctcgcct ctgctcggcc gtgacgagga
     7021 gctgcagcta cttctgcgtc gctgggcaca ggcggagcgc gccgatgggc gcgtggtgct
     7081 gctcagcggc gaggcgggca ttggcaagtc ccggctgatt cgggcgctcg agcaaagatt
     7141 gatcggagcg tcgcatcgag cgttcaacta ccacgcctcg ccgcatcagc aagacagtgc
     7201 gctccagccc atcattcaac agctgcgccg cgccgccagc atcgagcgcg aggatgcaag
     7261 cgaacggcag ctcgacaagc tcgacgcctt cattggggcg tgtggtggga aggtcgcggc
     7321 cgacctgccg gtcttcgccg cactgctttc gatcccggcg cacggccgct acgccctgcc
     7381 cgacttgccg ccgcaccggc tgaaggagcg ctttttgcag gcgctgctgc agctatgggg
     7441 tgagcttgcc cgcgacaaac cgttgttggt cgtgttcgaa gatgcgcact ggctcgatcc
     7501 cacctcgctc gagctccttt cgcgcgccgt cgatgccagt ttgcagtggc gcctgttgtt
     7561 gctggtaacc gcaagacccg aattcagcgc cccctggcca aaccatcaac atgtgtccag
     7621 tgtcgcgctt agccgactcg gccaaagcga gggcaaagcc ctggcccagg gacttgtgga
     7681 aaaggcgctg cccgaggccg tcttggccga gatcgtgcgt cgcacggacg gtgtgccgtt
     7741 gtttatcgaa gagctgacca agaccattct ggaaaccggt gtgctgcgtg aaggcgaggt
     7801 gcgctatgag ctcgacggcc cgctgccggc gctcgccatt ccagcgacgc tgcatgcctc
     7861 gctgctggcg cgactggacc ggctgcactc ggtcaaggat gtggtcgaga gcggcgcggc
     7921 gatcggccgg gagttcagct gggaccttgt ggcggcggtc tcctcgctct cccagcatga
     7981 tctgcagctt gccctcgaaa agctcgtgac cgcaggcctg attttccaac gcggcgtgca
     8041 gccgaatgcg actttcttat tcaagcacgc gcttgtgcgc gatgccgcct acgccggttt
     8101 gctgcgcggt cggcgcgcgc acgtgcacgg tcaaatcgcc aaggcgctgg aagggaggcc
     8161 ggaggtcgtt gccagcgaac cagagctgtt ggcgcatcac ctgacggagg ccggcttgtg
     8221 ggaggcagca cttccctact ggctgcaggc ggccgagcgc gccgttgcgc gttcggccct
     8281 gcgcgaagcg gcaaagcatt tccgggaagc gatcaggcta tcgcaacggc tgcagccatc
     8341 cctgcagcgc acgcgcagcg agctcgatgt gcatatccgg ttgggacccg tcgtcatggc
     8401 cacgcacggc tattccgcgc cggaaagtct cgagatttac aggcgtgcgg acgagctcgt
     8461 ggccgaggtt ggtgatgtcg tcgagcgcat ggatgtgctg cttggcttgt tcaacgtgca
     8521 ctatggccgc tgcgagctgc aacacgcgcg cgcaatcgcc gacgagcaca tgggattggt
     8581 ggagcgccac gccagcaacc ttggtcgcgg gcatacgctt ttggggcagc actatttggc
     8641 gatcggtgcc tttgtcgagg cgagccgaca tttccaggca gcgctggcga ttttcgccaa
     8701 aacgccggaa gacccttcgg tgctcggcgc cttcggcagc caacaggtcg tttgcctgtc
     8761 cctgatggcg ggcatccact ttgcccgcgg tgagatggat ttggcccagc aggcctcggc
     8821 ccgctccatc gagcttgcgc gttcatcgcg acataccatg tcgacggcac tggcgatcgt
     8881 caccgacctg ctgacgccgc tgccgggtgg gctcgacccc gaccccaaca aggcgcagga
     8941 ggtcgtggag ttttgccgcg agcacgggct caggaatttc gaagcctggg cgacctttgc
     9001 cgccggagcg gtcgcggcgc gacgggggga gccggccaaa gggattgcca tcatgcaagc
     9061 ggccatggcg acggcggagg gcatgggctc ccgcctgctc cgccccgttc agcttgccac
     9121 cttggcgatg gcgcacgcca agctcggcgc catcgaagac tgccttcgcc tcaacgaaga
     9181 ggccattgcc acggcggagc ggagtgggga aatgcaggcg gcggcgagcc tgctgcgcgc
     9241 ctatggcgaa gcccttctgc acctgggtcg ctctgacgag gcgaggcgcc aattcgagcg
     9301 cggtttcaag ctggccacag cgcagcaggc gaccttcgag gcgggtcgac tgaaggcgcg
     9361 cctcgagcgg ctcgcgcctt aaaagttttc gtcttgggca gcctcggccc gagcccgcga
     9421 ggttgctgcg gcaggtcagc gcgcgccgtt ggcagcaggc gctgcagagc gcgcgttggg
     9481 ctcaatgcgg cggcgcgtgc atgttccagt ccgttctgag gccgaccagc cgccgtatgc
     9541 gacggtccgc ggcggaaaaa gtggagcgaa gggcagcaag gctccagggc ttgagggcct
     9601 gcaaatggct gtcatcgagc gtgcagaaca gcgcgcagtt ttgaaagcgt gggttcaggg
     9661 tcgggttgcg gcacgccttg atcagcgctt cctcctgcgg cgtcatttgc gccacgcgtg
     9721 ggtcctgctc caacgccgct ctctcctcct cgcgccaggc cttaggtttc aaggccaggg
     9781 gcaggttgga gggtgcatag ctcttggcga gcttctcgtc gacgaaagcc gctcggccca
     9841 ggcgctcgat cacgctccaa tgaatttttt cgttgatcgg gatcatgttg cgcttttctt
     9901 ttttaccctt gaaccaagcc tcgatcggcg ttggttctgg gaagagcggt cgtcggtagg
     9961 gaacgaggct gctgaaccac caccaaccgc ggctggagcg gtagagcgag caggcggcgc
    10021 agggccagaa gttcttggcg atgtagtctg ggctgaacct cagcttggtc aaggcctcga
    10081 cccttgtcat gatccagatc agcgccaaat cggacaggcc gctgcgcaga taaccgccgc
    10141 ccacattggc atgcacgcct gcaaaccaga cctgttcgac gtgctggtgc gcgttcggct
    10201 cgttcgggtc gccgacccaa gtggttggcg gaaacgcggg gcgcaactcg tcgatggcaa
    10261 gcgcttgcag ccccacttcg atgcgcgaat tgagcatgtt gtcgtgaaag ccgcgggtcc
    10321 aagaggtgag cttgcgcgcc aggcccgaca agccgaggcc agcgggaatg ccgtaggagc
    10381 ccaccgtgtc ccacaccgcc aggcatttga tcagcggtgc gggccgcggc acttcggcga
    10441 ccatcttgtc gatttcgtat ttgtgccggg cctcatcgct gtagcgctcg cgtgggctca
    10501 agcggtagaa attccaaatc tcctcgaggc gatgaaagta ctcctggcgg gggacacctc
    10561 ggatggcggt gatcacgccg gcgagcgcgc gcgcggtata ggccccgcgc gagaagccga
    10621 agatgtagat ctcgtcgccc tccgcccaat tgagggtgag aaaggcgagc ccgcgtttga
    10681 cgttggccct gacgccggcg ccaaagacgc cgccgagaaa gcggtcgata gggccaccgc
    10741 tgccaacgcc ggcgttgtag tagacgattt ggctgcggcc ctgttcgtcg gcggcccgga
    10801 cggcctggcc tactctgacc acgttggtga cggcgctggg atcggtcagc gcgttccagg
    10861 tgccgtcata gcaaacgacg atgcgtttca tggcttcccc ctgtcggctg ctgggcgcga
    10921 gcgcgacgaa tgacaaaacg cagggggcgc gcgcgcgcct cgcagcgcgg ccccaacccc
    10981 cgggaagtgt aagcctctcc ggcgtcggat cggcaagcaa gaggcggcaa taaaccaggc
    11041 ccgtggtatt cccggtacat tgccgccgcg gatctccgcg ccgctcgagg ttccgccagg
    11101 taggccgtgg cggcgcaggg acactatcgc cgccaagccc agccggcgct gtgctgttgg
    11161 gcacagctcg gccgggcgcg gaactcgccc ttgcgcgcgt tcacgcgccg tcaccgcgat
    11221 ggcttgctgg tcccagtcag cgccgcgatc cggtcggcga cgaagcgctc catcaagtgc
    11281 ggtgccgagg tgccaaaggt gcgcatgcga cccgtgtgcg ccagcagtaa aagtccggcg
    11341 gcgtgggcga aggtctcaac catcaagacc ttggcgtcat cgccacttgc ccccagtgcc
    11401 agggcggcgt cggcgatcgg cgccaaggcg gcgcacaggg cagcgttgag ggtctcatcg
    11461 cgctctttgc ccaggccttg cggcttcatg ccgccgcgga acagatagaa gccgagatcg
    11521 aggtcgcgcg gattgtcgtc gtagtaacgg aagaaggtga gcgcagccgc ccgcagccgt
    11581 tgcgccggtg tcctggcgcg cagggtcgcg cgg
//
LOCUS       DASTMT010000520         2510 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_6385906, whole genome shotgun sequence.
ACCESSION   DASTMT010000520 DASTMT010000000
VERSION     DASTMT010000520.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2510
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6385906"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..397
                     /locus_tag="VFR00_11955"
     CDS             <1..397
                     /locus_tag="VFR00_11955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019963238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEU0160026.1"
                     /translation="AVGNTYYMALMATNEKNPEQKRWGAAIKPLFPNANDRGSHVNIS
                     GMALAKNAPNKANALKLMEFLASDAAQKIYASANNEYPVNPAVEPSAIVRSWGKLKPD
                     ALPLENIAKYRKKASELVDKVNFDGGPSS"
     gene            650..1396
                     /locus_tag="VFR00_11960"
     CDS             650..1396
                     /locus_tag="VFR00_11960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002711524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HEU0160027.1"
                     /translation="MVADSHRRSVRTPPPARPSLAGLDWSAIARDLDSNGCAVVGPLL
                     DAATCRALRASFALEAAFRSHINMARHGFGQGEYKYFAHPLPASVGALRSALYPPLSE
                     IANRWYALMSMENRFPSEHQAFLETCHAAGQTKPTPLLLRYGPGDYNCLHQDVYGELV
                     FPLQVAVLLSAPGTDFTGGEFVLTEQRPRRQSRAEVAPLQQGEAVIFPVRERPVQGSR
                     GIYRVNMRHGVSRLRSGNRYTLGIIFHDAH"
     gene            complement(1414..>2510)
                     /locus_tag="VFR00_11965"
     CDS             complement(1414..>2510)
                     /locus_tag="VFR00_11965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009735269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEU0160028.1"
                     /translation="LVAAATAVLTREGSSLATYGLNSGPLGYRPLREFLAGKLKWHAG
                     IACEPDEILITSGSLQGLDLVNSLLLAPGDTVLVEEDCYSGSLSRLSRRGVQALGVPL
                     DSGGLRLDALAEMLTDLKRRGVRPKYIYTIPTVQNPSGTIMTEERRVELLRLSRAHGL
                     PIFEDECYSDLIWDGRRPPALYALSNGVGVIHIGSFSKSISPALRVGYLVASWDVLSR
                     ILALKTDAGSGALEQMVLAEFCAAHFAEHVRKLNRGLHAKLETLTAALAEQFGTAAEF
                     EVPKGGIFLWLKLPENVDTQKLAQVALAEGLAINPGGEWSTNKERGRTRLRLCFANPE
                     AQTIRRGVAALAEICQREFGVPTRIANIKR"
BASE COUNT          456 a          838 c          800 g          416 t
ORIGIN      
        1 ggccgtcggt aacacctatt acatggcctt gatggcaacc aacgagaaga accccgaaca
       61 gaagcgctgg ggcgccgcca tcaagccgct gttccccaac gccaatgatc gcggcagcca
      121 tgtcaatatt tcgggcatgg cgctggccaa gaacgcgccc aacaaggcga atgctttgaa
      181 gctcatggag tttttggctt ccgatgcggc gcagaagatc tacgcgagcg ccaacaacga
      241 ataccccgtc aaccccgcgg tcgagccgtc ggcgattgtg cgcagctggg gcaagctgaa
      301 gcccgacgcc ttgccgctgg aaaacattgc caaatatcgc aagaaggcat cggagctggt
      361 cgacaaggtc aatttcgacg gtggcccgag ctcctaaagc aagctcctaa ggcggacgcg
      421 cccggcgttc gcgcctccgc aacggctcca tggctccccc tggcgccgcg cctgtcgatg
      481 ggcgcctggc agcgcggcgc aggcttggct gggggggtcc ggtcgcatag ccgtcgtcgc
      541 cgcgacggag atccgcgcgc cgaaaacggg tcgcccggcg ccggcctgca tggcaaggtc
      601 ctgggcgtaa ggggttctgg ccccgttttg ttaacgccga gatccgagca tggtcgccga
      661 cagccaccgc cgcagcgtcc gcacaccgcc gccagcccgc cccagccttg caggcctcga
      721 ctggagcgcc attgcccgtg acctcgacag caacgggtgt gccgtcgtcg gcccgctgct
      781 ggacgcggcg acctgtcggg cgctcagggc ctcgtttgcc cttgaggcgg cgtttcgcag
      841 ccacatcaac atggcacggc atggtttcgg gcagggcgag tacaaatatt tcgcccatcc
      901 gctcccggcc agcgtcggcg cactgcgcag cgcgctttat ccaccgctgt ccgagatcgc
      961 caaccgctgg tacgcgctga tgtcgatgga aaatcggttc ccctccgagc atcaagcctt
     1021 cctcgagacc tgccatgccg ccgggcaaac caagcccacg ccgctgctgc tgcgctatgg
     1081 gcccggcgac tacaattgcc tgcaccagga cgtctatggg gaattggtct ttcccctgca
     1141 ggtcgcggtg ctgttgtccg cgcctggcac ggatttcacc ggcggcgaat tcgtcctgac
     1201 cgaacaacgg ccgcgccggc aatccagggc cgaggtggcc ccgctgcagc agggcgaggc
     1261 cgtcattttc cccgtacgcg agcggcccgt gcaaggcagc cgcggcatct accgcgtcaa
     1321 catgcgccac ggtgtgagcc ggctgcgcag tggcaatcgc tacacgctgg gcatcatttt
     1381 ccacgacgct cactgagccg gacgcgcgcg ctttcagcgt ttgatgtttg caatccgcgt
     1441 cggtacgccg aattcacgct gacaaatctc cgccagggcg gcaacgccgc ggcggatcgt
     1501 ttgggcctcc ggattggcga aacacaggcg caggcgcgtc ctgccgcgct ccttgttggt
     1561 ggaccactcg ccaccgggat tgatggcgag gccctcggcg agcgcaacct gggccagttt
     1621 ctgagtatcg acgttctccg gcagtttgag ccagaggaag atgccgccct tcggaacttc
     1681 gaattcggcg gcggtgccga actgctcggc caacgcggcg gtcagggttt cgagcttggc
     1741 gtgcaagccg cggttgagct ttcgcacatg ctcggcaaaa tgcgccgcac agaattcggc
     1801 cagcaccatc tgctcgaggg caccggagcc cgcgtcggtc ttcagcgcca aaatgcgcga
     1861 cagaacatcc cagctcgcga ccaggtagcc cacgcgcagc gccggcgaaa tcgacttcga
     1921 gaaggagccg atgtgaatga cgcccacccc gttgctgagg gcatagagcg caggcgggcg
     1981 ccgaccatcc cagatcaggt cggagtagca ttcgtcctcg aagatcggca acccgtgcgc
     2041 ccgcgacagg cgcaacagct cgacccggcg ctcctctgtc atgatcgtgc cgctcggatt
     2101 ttgcacggtt gggatcgtgt agatatactt cgggcggacg ccgcgacgtt tgagatcggt
     2161 gagcatctcg gcaagcgcat cgaggcgcaa gccgcccgaa tcgaggggca cgcccaacgc
     2221 ctgcacgccg cgccggctaa gccggctgag cgaaccgctg tagcagtcct cctcgaccag
     2281 cactgtatcg ccgggggcca gcagcaggct gttgacgaga tcgagccctt gcagagagcc
     2341 cgaggtgatg aggatctcgt ccggctcgca ggcaataccg gcatgccact tgagtttgcc
     2401 tgccaggaac tcccgcagcg gccgatagcc gagcgggccg ctgttcaagc cataggtggc
     2461 gagcgaagat ccctcgcgcg tgagaacggc ggtcgcggcg gcaaccaatt
//
LOCUS       DASTMT010000521         5864 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_1608630, whole genome shotgun sequence.
ACCESSION   DASTMT010000521 DASTMT010000000
VERSION     DASTMT010000521.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 5864)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5864)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5864
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1608630"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..295)
                     /locus_tag="VFR00_11970"
     CDS             complement(<1..295)
                     /locus_tag="VFR00_11970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0160029.1"
                     /translation="MRRVDGRVGRLICGLLLASVAASSGASAQGWPSWADDLFGTGRG
                     RIEPRRDTFEAPQREPRRDLERGQRNQVGGDVREGGPRPAIIPVAPATVTFDQD"
     gene            complement(506..2167)
                     /locus_tag="VFR00_11975"
     CDS             complement(506..2167)
                     /locus_tag="VFR00_11975"
                     /inference="COORDINATES: protein motif:HMM:NF012431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEU0160030.1"
                     /translation="MPISTTVADRLAADLAAYGARRCFGLLGTANFKISHALVAAGVE
                     LISARHEMNAASMADAYAKATGELTLVSVHCGPGLSNALTGIGEAAKSRTPLLVLAGD
                     VPAGAVTSNFYIEQAEMVRSVGAAAERIHTPATARQDTLRAVLRALRERTTVVLSLPT
                     DVQDAACSGAAPPLTLPPAPAPANPDPETLRALAEALEAAQRPLLLAGRGAVVAGAEQ
                     ALAALAAFSGALIATSVCGHGLLAEDAWCLGISGGFASPAADALISNSDLIVGFGVSL
                     TPWTTKRGKLIAAEAVVAQVDVDPARFGRHTRLDHAVLGDARVTAEALLAELQRRRAA
                     ARSGWRDAATRQCLEAGSNHTAPYQDVSTAERIDPRTLSKAVDAILPRERVVASDSGH
                     FCGWVPRYLRVPNARASCLSHSFQSVGLGLASVIGLALANPGALAVLGAGDGGFLMSI
                     SDLETALRLKLRLCILIYNDSSYAAEVHLYRRRGYDVDIVQFPETDFAAIARGFGCRG
                     VSVRTLHDLEPVRAWVSEGCPGVLLIDAKISRDLEADWHAEHFPE"
     gene            complement(2173..3489)
                     /locus_tag="VFR00_11980"
     CDS             complement(2173..3489)
                     /locus_tag="VFR00_11980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018700611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEU0160031.1"
                     /translation="MSWQMAALLLLGGSTVLLFLGLPVALSFVAINIVGAMLFLGGDP
                     GLSQVVRNSVVSLMNFSLTPIPLFILMGELLFHTGLALKVIEGVERLIRQVPGRLAVI
                     AVVAGTVFSAISGSTIATTAMLGSLMLPVMLARGYHPAFATGPIMAIGAVDMLIPPSA
                     LTVLLGSLSGISISKLLIGGVVPGLILSAAFIAYIILSALRAPDLAPPAPPPQQARGE
                     SWRLLVAYVVPLVSIFVVVVGAMSGGWATPTESAALGAIATLLLALAYRALTLAALQA
                     ALRGTVAITGMILFIILGATTFSQILSFSGATEGLVATILGQGLSAFAVLVGMMLLLI
                     FLGIFVDQVSMMLITLPVFMPIVGRLGIDPIWFGVLFLICMQLGLLLPPHGLLLMTMR
                     GVAPPEVTMGHIFRAVVPYVVMSLLLLAVLLLLPAIATWLPNRLIG"
     gene            complement(3528..4043)
                     /locus_tag="VFR00_11985"
     CDS             complement(3528..4043)
                     /locus_tag="VFR00_11985"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HEU0160032.1"
                     /translation="MTVLSRWYGAMLDALMGLAALLVLGMTLMIGGDVLSRNIGYGGI
                     AWSNEVSEYILYLVTLLSAPWLLRRGQHIRVDILLRALPLRLAYALEWVADVLGLACA
                     LYFVAWGWRVAAASYAAGAISLKTLVLPEWWMLACLPAAFLLVAIEFCFRMHRLAQAE
                     VGPRADAVSAS"
     gene            complement(4072..5025)
                     /gene="dctP"
                     /locus_tag="VFR00_11990"
     CDS             complement(4072..5025)
                     /gene="dctP"
                     /locus_tag="VFR00_11990"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HEU0160033.1"
                     /translation="MQGWMGMALAASLWLASTLTAGAEEVTLKGITSFAEKTFYSRGF
                     ERFIDKVNADGKGLVQISYIGGPKAMPPFEVGNALKSGVVDLANVTGAFYTNVMPEAD
                     AWKLTQKPMAELRQDGGYAYMANLYEQKMNAILLARHIDNNPFHLYLTKPISGPDLTG
                     LKLRITPVYRDFFQALGATVVQTAPGEVYTALERGVVDGYGWPITGIFDLGWHEKTKF
                     RVDPGFYSAEVSILINKNAWAKLNDRQKQVIADAAAWIEAQAADVAKENEADIAKQKA
                     AGIEVITLPDDFRSKAYEAGWAGVIRQSPEHGPKLKALFSK"
     gene            complement(5200..>5864)
                     /locus_tag="VFR00_11995"
     CDS             complement(5200..>5864)
                     /locus_tag="VFR00_11995"
                     /inference="COORDINATES: protein motif:HMM:NF039910.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEU0160034.1"
                     /translation="RSAGPGRTYWRFANFLFPCWSQAPNGEFDSHMHARAWVPLDDGH
                     CMFVFIFWKHAKSAQALPQPRFKDGTPIGGTGRGNIKMLPNTTDWLGRWRMALDETND
                     WGMDREAQQSNKIYSGIDGVHLQDQAITESMGPIVDHSFEHLAPSDQMITRTRRRLLL
                     AARALRDKAVTPPGAEEPEVYRGARSGYFISDDQRPWQEVYARQLAAAVRPPAEPLHA
                     AE"
BASE COUNT         1093 a         1950 c         1954 g          867 t
ORIGIN      
        1 aatcctggtc aaaggtcacc gtcgccggcg cgaccgggat gatcgcgggc cgcggcccgc
       61 cctcgcgcac atcgccgccg acttggttgc gctgacctcg ctcgagatcc cggcgcggct
      121 cgcgctgggg cgcctcgaag gtgtcgcgcc ggggctcgat gcgtccccgc ccagtgccga
      181 acaggtcatc ggcccaggag ggccaaccct gcgccgaggc tccactgctc gcggccacgc
      241 ttgccaacag gagaccgcag ataagcctgc caactctgcc atcgaccctg cgcatgcgtg
      301 ctgatccctg cgcttagaaa gcatggaagc taaccccgat ttgccgcgga tcgttccgcc
      361 gctagcggcg gaattcacgg ggagccggca ggtcagctgc aaaggcgcac gcgggcaacg
      421 ccgtgccgcg tcaccgcggt tttgcgacga cgttgccgcg cgagctgcgc cgaggttgcg
      481 tccgcggccc gcctctgcct tgcactcact ccggaaaatg ctcggcgtgc cagtcggcct
      541 cgagatcccg gctgatcttg gcgtcgatca gcagcacgcc ggggcaaccc tcgctcaccc
      601 aggcgcgcac cggctcgagg tcgtgcagcg tgcgcacgct aacaccgcga cagccgaagc
      661 cgcgcgcaat cgcggcgaaa tcggtttcgg ggaactggac aatatcgacg tcgtagccgc
      721 ggcgacgata aagatgcacc tcggccgcgt aggagctgtc gttataaata agaatgcaaa
      781 gtctgagctt gaggcgcagc gccgtttcga gatccgagat cgacatcaag aaaccgccgt
      841 cgccggcgcc cagcacggcc agcgcgccgg ggttggccaa cgccaaaccg ataaccgagg
      901 cgagcccgag accaacggac tggaaggagt gactgagaca ggaggcgcgc gcgttgggaa
      961 cgcgcaaata gcgcggcacc cagccgcaga aatgcccgga atcggaggca accacgcgct
     1021 ccctcggcaa aatcgcatcg acggctttgc tcagcgtgcg cgggtcgatg cgctcggcgg
     1081 ttgaaacgtc ctggtagggg gccgtgtggt tcgagccagc ctcgaggcac tggcgcgtgg
     1141 cggcatcgcg ccagccggac cgcgctgcgg ctcggcgccg ttgcagctcc gccagcaacg
     1201 cctcggcggt gacgcgcgca tcgccgagca cggcgtggtc gaggcgcgtg tgccgaccaa
     1261 accgcgccgg gtcgacgtcg acctgggcca cgacggcctc ggcggcgatc agtttgccgc
     1321 gcttggtggt ccacggcgtc aagctcaccc caaagcccac gatgagatcg ctgttagaga
     1381 tcagcgcgtc cgccgccggc gaggcaaagc cgccgctgat gccgaggcac caggcatcct
     1441 ctgccaggag gccgtgccca cagaccgagg tggcgatcag ggccccgctg aaggcggcca
     1501 aggcggccag cgcctgctcg gcgccggcga cgaccgcgcc gcgcccggcc agcaggagcg
     1561 gacgctgcgc tgcctccagc gcttcggcca aggctcgcag cgtctcggga tcgggattgg
     1621 ccggcgccgg cgcggggggc aaggtgagcg gcggcgccgc gccggagcaa gcggcgtcct
     1681 gcacgtcggt tggcaaactc agcaccaccg tggtgcgctc gcgcagcgcc cggagcacgg
     1741 ctctcagcgt gtcctgccgc gccgtggcgg gcgtatggat gcgctcggcg gcggcgccga
     1801 ccgatcgcac catctctgcc tgctcgatgt agaagttcga ggttaccgcg ccagcaggca
     1861 cgtcgccggc caggaccagc aggggcgtgc ggctcttggc ggcttcgccg atgccggtca
     1921 aggcgttgct gaggcccggc ccgcaatgca cgctgacaag cgtcaactcg cccgtcgcct
     1981 tggcgtaggc gtcggccatg ctcgcggcat tcatctcatg gcgcgcggaa atgagctcga
     2041 cacccgcggc gacgagcgca tgcgagatct tgaaattggc cgtgcccagc aggccgaagc
     2101 aacggcgcgc gccataggcg gccaaatccg cagcaagccg gtcggcgacc gtggtcgata
     2161 tcggcatcgt gttcatccaa tcaagcggtt cggcagccaa gtggcgatgg ccggcagcag
     2221 cagcagcacg gccagcagca gcaggctcat caccacatag ggcacgacgg cacggaaaat
     2281 gtggcccatc gtcacctcgg ggggcgcaac gccacgcatg gtcatcagga gtagcccgtg
     2341 cggtggcagg agcaggccaa gctgcatgca gatgagaaac agaacgccaa accagatcgg
     2401 gtcgatgccg agcctgccaa cgatcggcat gaaaaccggc agcgtgatca gcatcatgct
     2461 gacctgatcg acaaagatgc cgagaaatat caacagcaac atcatgccga ccaggacggc
     2521 aaaggcggag aggccctggc cgaggatggt cgccaccaat ccttccgtgg ctccggaaaa
     2581 gctcaagatt tgcgaaaacg tggtggcccc gaggatgatg aacaggatca tgccggtaat
     2641 ggccacggtg ccgcgcagcg ccgcctgcaa agccgccaac gtcagggcgc gataggcgag
     2701 ggcgagtagc agggtggcaa tggcgccgag tgcggccgat tcggtggggg tggcccagcc
     2761 gcccgacatg gcgccgacga cgaccacgaa gatcgaaacc aacggcacca cataggcgac
     2821 cagcaggcgc caactctcgc cgcgcgcttg ctgcggcggc ggcgccggcg gcgcgagatc
     2881 tggcgcgcgc agcgcgctga ggatgatgta ggcgatgaag gccgcgctga ggatcagccc
     2941 cggcacgacg ccaccgatca gcagtttgga gatggaaatc cccgacaggc taccgagcag
     3001 cacggtcagc gccgacggcg gtatcaacat gtcgacggcg ccgatcgcca tgatcggacc
     3061 ggtggcgaag gcgggatgat agccacgcgc caacatcacc ggcagcatca aagagcccag
     3121 catggccgtg gtggcgatgg tcgagcccga gatggcggaa aataccgtgc cggccaccac
     3181 cgcaatgacg gcgagccgcc ccggcacctg ccggatcaac cgctccacgc cctcgatcac
     3241 cttgagcgcc aatcccgtat gaaacaggag ctcccccatc agaataaaga gcgggatcgg
     3301 ggtgagcgaa aagttcatca gcgatacgac gctgttgcgc acgacttggc tcaagccggg
     3361 atcgcccccc agaaacagca tggcgccgac gatattgatg gcaacgaagg agagcgccac
     3421 cggcaggccg agaaacagga gcacggtcga gccgccgagc agcaggagtg cggccatctg
     3481 ccaggacaac gctttacccc ttatgcgtct gagccttcat ttcgcgctta cgacgccgac
     3541 accgcatccg cgcgcggacc aacctctgcc tgcgccagcc ggtgcatgcg gaagcagaac
     3601 tcgatggcca cgaggaggaa ggctgcgggc aggcaggcca acatccacca ctccggcagc
     3661 accagggtct tcaggctaat cgcgccggcc gcgtaactgg cggcggcaac ccgccaaccc
     3721 caagcgacga agtagagcgc acaggccaaa cccaaaacat ccgccaccca ttccagcgcg
     3781 taggccaggc ggagcggcag tgcgcgcaag agaatatcga cgcggatgtg ctggccacgc
     3841 cgcaacaacc agggcgccga cagcagcgtg acgaggtaga gaatgtactc ggacacctcg
     3901 ttgctccagg cgatcccgcc atagccgatg ttgcgggaga gcacgtcgcc gccgatcatc
     3961 aacgtcatgc ccagcacgag cagcgccgcc aggcccatca gggcgtcgag catggcgcca
     4021 taccagcgcg agagcacagt catccccgtc gcccgccttg tttgcgcgca cctatttgga
     4081 aaagagcgcc ttcagcttcg gcccatgctc cgggctctgg cggatcacgc cggcccatcc
     4141 cgcttcgtag gccttgctgc gaaagtcgtc gggaagcgta atgacttcga tgcccgccgc
     4201 cttctgcttg gcgatatcgg cctcgttctc cttggcgacg tcggcggcct gcgcctcgat
     4261 ccaggccgcg gcatccgcaa tgacttgctt ttgccgatcg ttcagcttgg cccaggcgtt
     4321 cttgttgatg aggattgaga cctcggcgct gtaaaagccc ggatcgaccc taaacttcgt
     4381 cttttcgtgc cagccgaggt cgaaaatgcc cgtgatcggc cagccatagc cgtcgacgac
     4441 gccacgctcg agcgcggtat agacctcgcc cggggcagtc tggaccacgg tggcgccgag
     4501 cgcctgaaag aaatcgcgat agacgggcgt gatccgcagc tttaggccgg tgagatcggg
     4561 cccggaaatc ggctttgtca aatagagatg gaaggggttg ttgtcgatgt gccgggcgag
     4621 caggatggcg ttcatcttct gctcgtagag gttggccata taggcatagc cgccgtcctg
     4681 gcgcagctcg gccatcggct tttgcgtcag cttccaggca tcggcctcgg gcatcacgtt
     4741 ggtgtagaag gcccccgtca cgttggcgag atcgaccacg ccgcttttca aggcattgcc
     4801 cacctcgaac ggcggcatgg ctttcggccc gccgatatag ctgatctgca ccaggccttt
     4861 gccgtcggca ttgaccttgt cgatgaagcg ttcgaagccc cgagaataga aggtcttttc
     4921 cgcaaacgag gtgatgccct tgagcgtcac ctcctcggcg cccgcggtga gcgtcgaggc
     4981 gagccacaaa gacgccgcca gcgccattcc catccaaccc tgcatacgcc tgctccctag
     5041 ccagtggccc tgccgttgcg accccgaggc caacaagagc acagcgcccg gccaatccgc
     5101 aacttgctcg gacagcaccg gcggcagcaa agccggtgac gcggcgccgg cgggccgtgc
     5161 gccgcgccgt gcttgctggc tttgaggctg gagcgcgcgc tactcggcgg cgtgcagggg
     5221 ctcagccggc gggcgcaccg cggcggccag ctgccgggca tagacctcct gccatgggcg
     5281 ctggtcgtca ctgataaagt agccgctgcg ggcaccgcga tagacctctg gctcctcggc
     5341 gccgggcggc gtgacggctt tgtcgcgcaa ggcgcgggcc gccagcagca gccgccgccg
     5401 cgtgcgcgtg atcatctgat ccgacggggc caggtgctcg aagctatgat cgacgatggg
     5461 acccatgctt tcggtgatcg cctggtcctg caggtggacg ccgtcgatgc cgctgtagat
     5521 cttgttgctc tgctgcgcct cgcggtccat gccccagtcg ttggtctcgt ccagcgccat
     5581 gcgccagcgg ccgagccaat ccgtggtgtt gggcagcatc tttatgttgc cgcggccggt
     5641 gccgccgatc ggcgtgccgt ccttgaacct gggctgcggc agggcctgcg ccgacttggc
     5701 gtgcttccag aagatgaaca cgaacatgca gtgtccgtcg tcgagcggca cccaggcgcg
     5761 ggcatgcatg tggctgtcga actcgccgtt gggcgcctgc gaccagcagg ggaacaggaa
     5821 gttggcgaag cgccagtagg tgcggccggg cccggcgctg cggt
//
LOCUS       DASTMT010000522         5341 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_4417553, whole genome shotgun sequence.
ACCESSION   DASTMT010000522 DASTMT010000000
VERSION     DASTMT010000522.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 5341)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5341)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5341
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4417553"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..676)
                     /locus_tag="VFR00_12000"
     CDS             complement(<1..676)
                     /locus_tag="VFR00_12000"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEU0160035.1"
                     /translation="MGASAEEWLERFLEMQAAERGAAANTIGAYRSDLKHFLGYLEAR
                     GKLPGAVASRDIGAYQRQLAVAGMAPSTRARRLAAVRQLFKFLLAEGAIADDPAGRIA
                     GPRRNRTLPKTLSIGEVDRLIAAAAARTRTTQGREHLRALRLNALLEVLYATGLRVSE
                     LVSLPRSVLDGDGKVLIVRGKGGRERMVPLTLSARTALDDYLNAPRPAGDGGRKGRFL
                     FASRSSE"
     gene            complement(706..849)
                     /locus_tag="VFR00_12005"
     CDS             complement(706..849)
                     /locus_tag="VFR00_12005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0160036.1"
                     /translation="MPSLIRFLVVVSVIGAAVIGGLYVTAVFFEPAQRETSTPLPGVK
                     VRR"
     gene            1046..1690
                     /locus_tag="VFR00_12010"
     CDS             1046..1690
                     /locus_tag="VFR00_12010"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018387452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEU0160037.1"
                     /translation="MMRFGYGGKKEEQIAQLAGKLRAGLGTRSVVLVGMPGCGKSAIG
                     RRLASRLELPFVDADDEIELAAGKAITDIFKEHGEPYFREGERKVIARILNSGSQVLA
                     TGGGAFMSAETRENIRNAAVSVWLKAELTLLLRRVLKRNNRPLLERDPEGVMRSLVET
                     RYPIYATADITVESRDLPHDVMVFEIIEALAAHTLAPQPEPSVDAASRSSGEPI"
     gene            1687..2880
                     /gene="aroB"
                     /locus_tag="VFR00_12015"
     CDS             1687..2880
                     /gene="aroB"
                     /locus_tag="VFR00_12015"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011156071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEU0160038.1"
                     /translation="MSKTPEATTKQDEVTRHAAGRGEIGGEDALVGVRVALEGRAYDV
                     LVGPQLATRAADLIDKRLGPARCAIVTDANVAATHLARVAAGLDAMGRLAGSKILPPG
                     ESSKSFPVLAELCESLLAMQLERGDLLLALGGGVIGDLAGFAASILRRGIRYVQLPTT
                     LLAQVDSSVGGKTAINASHGKNLIGTFHQPSLVLADIDLLATLPARQFRAGYVEAAKY
                     AILADAAYFDWLEANRASLFNREPAALSQAIGIAVAGKAAIVARDETETGERMLLNLG
                     HTFGHALEAWAGYSDKLLHGEAVAIGITLAFELSHELGFSDAESAERVRTHLASAGLP
                     TRIKDIAGNAWPEADSLLRLMAQDKKVRAGQLTLILARGIGKAFATREVDTATVRDFL
                     ARRIG"
     gene            3056..4375
                     /locus_tag="VFR00_12020"
     CDS             3056..4375
                     /locus_tag="VFR00_12020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019402542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HEU0160039.1"
                     /translation="MSLDIALILTAIAVLLLLSAFFNGSETALTAVSRARMHALEQEG
                     NSRARLVNHLLKEPERLIGVVLLGNTLVDILAASLASGLAVILIGPAGVAYATAVMTI
                     LIVIFAAVLPKTYAFAFADRLALFVAPLMRVMIAVLNPATRAIQVIVRMLLKLTPTSR
                     DDEANILEAHAEIRGTIELQKIEGAVAKRDAAMLGGVLDLKDLKVLDIMVHRTKMQTI
                     NISDAPEKVIDEVLKSQFTRVPLWKDEPENIVGVLHTKDLLLALSRAGWEVAKIDIAA
                     VAAQPWFVPDTTSVANQLNEFLRRKAQLALVVDEYGEVQGLITLEDILEEIVGQIADE
                     HDVQDAAIRLQADGSVNVDGTVAVRDLNRHMDWDLPDEEATTIAGLVIHEAQSIPEPG
                     QAFTFYGYRFEILRKNRNKITAIRVRPVTAKQRRPRSSLESDAMDAG"
     gene            complement(4389..5150)
                     /locus_tag="VFR00_12025"
     CDS             complement(4389..5150)
                     /locus_tag="VFR00_12025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEU0160040.1"
                     /translation="MLRSLLFVAVFYLNTAVFLLFGSWLLLAPRRWAMQGLRLHGLAS
                     LWWLKIICGTKVEVRGREKLPRGACLVAAKHQSAWDTFGLIPVFRDPAMVMKAELGLI
                     PLYGWFSHKFQHIFVKRDKGPGALKSMIRDARNRAAAAREIVIFPEGTRRPPGAPPDY
                     KPGFIALYEGLGLPCVPLALNSGLFWPRRSLMRYPGTIVVEILEPVPPGLARAEARAL
                     IEARIETACARLLAEARAAPNPPPLPAMAPLRASP"
BASE COUNT          958 a         1739 c         1778 g          866 t
ORIGIN      
        1 cctcgctgct gcgcgaggcg aagagaaagc ggcccttgcg cccgccgtcg ccggccgggc
       61 gcggggcgtt cagataatca tcgagggcgg tgcgggcgct gagggtgagc ggcaccatgc
      121 gctcgcggcc gcctttaccc ctgacgatca aaaccttgcc gtccccgtcg agtaccgagc
      181 ggggcaagct caccaactcg gagacccgca agcccgtggc gtacaagacc tcgagtagcg
      241 cattgagccg cagcgcgcgc aggtgttcgc ggccctgcgt tgtgcgggtg cgggccgcgg
      301 ccgcggcgat gagccggtcg acctcgccga tcgacagcgt tttgggcagc gtccggtttc
      361 ttctggggcc ggcgatgcgg ccggcggggt catcggcgat ggcaccttcg gccagcagga
      421 acttgaagag ctgacgcacc gccgccagcc gccgtgcccg cgtgctgggc gccatgcccg
      481 ccaccgccag ctgccgctga taggcgccga tgtcgcggct tgcgaccgcg cccggcagct
      541 tgccgcgggc ctcaagatag ccaaggaagt gtttgaggtc gctgcggtag gcgccaatgg
      601 tattggctgc cgcgccccgc tcggcggcct gcatctccag aaatctctcg agccattcct
      661 ctgcgctggc gcccattgtg ccccaacagc gttaagttgc tggcgctaac ggcggacctt
      721 gacgcccggc aagggcgtgg aggtttcccg ctgcgccggc tcgaagaaca ccgccgtcac
      781 gtaaaggccg ccgatcacgg ccgcgccgat cacgctcacc acgaccaaaa accggatgag
      841 gctcggcatg tcgcaaccct gctcctgatc ccggccgtcg tcggtgccca aatgccgccc
      901 aaatcggccg aacctacgga caggacgcgg gacgccgggt gcccccgagc gcgcccatga
      961 ccgcggtgcg gctttgaggc ctacccgcaa acctgatacc agaaagcatg gagggtggca
     1021 gcaagcgccg tcgtgcagga cagagatgat gcgattcggc tacggaggca aaaaggagga
     1081 gcagatcgcc cagctcgccg gcaagctgcg tgccggactg ggcacgcgct cggtcgtgct
     1141 ggtcggcatg ccgggctgcg gcaaatcggc catcggccgg cgcctcgcca gccgcctcga
     1201 attgcccttt gtcgacgccg acgacgagat cgagctcgcc gctggcaagg ccatcaccga
     1261 catcttcaag gaacacggcg agccctattt ccgcgagggt gagcgcaagg tgattgcgcg
     1321 catcttaaac tccggctcgc aggtgcttgc gaccggcggt ggcgccttta tgagcgcgga
     1381 gacccgcgag aacatccgca acgcagcggt ctcggtttgg ctcaaagccg agctcaccct
     1441 acttctgcgg cgcgtgctga agcgcaacaa ccgtcccctg ctcgagcgcg atccggaagg
     1501 cgtcatgcgc tccctcgtgg aaacgcgcta tccgatttac gccacggcgg acattacggt
     1561 cgaaagccgc gacctgcccc acgacgtgat ggtcttcgag attatcgagg ccctggctgc
     1621 acacaccctg gcaccgcagc cggaaccttc cgtcgacgca gcatccagaa gcagtggcga
     1681 gccaatatga gcaagacccc cgaggcgaca acaaaacagg acgaggtcac gcgccacgca
     1741 gcgggccgcg gcgagatcgg cggcgaggat gcgctggtcg gcgtcagggt ggcgctggag
     1801 ggtcgtgcct acgatgtgct cgtcgggccg cagctcgcga cgcgcgccgc cgatctcatt
     1861 gacaagcgcc tcggccctgc gcgctgcgcg atcgtcaccg acgccaatgt cgcggcgacg
     1921 caccttgcgc gcgtcgcggc tgggcttgat gcgatggggc ggttggccgg cagcaagatc
     1981 ctcccgcccg gcgaaagcag caaaagtttt cccgtgcttg cggagctgtg cgagagcctg
     2041 ctggcaatgc agctcgagcg cggcgatctg ctgctggcgc tcggcggcgg cgtgatcggc
     2101 gatcttgccg gctttgccgc cagcattctg cggcggggga tccgttacgt gcagctgccc
     2161 accacgctgt tggcgcaagt ggattcatcg gtcggcggca agaccgcgat caatgcctcg
     2221 cacggcaaga acttgatcgg caccttccat cagccgagcc tggtcctggc cgatatcgac
     2281 ctgctggcga ccttgccggc acgccagttt cgcgccgggt acgtcgaagc ggccaaatac
     2341 gccatattgg ccgatgcggc ctatttcgat tggctcgagg cgaaccgagc ctcgctgttt
     2401 aaccgcgagc ccgcggcctt gagccaggcg atcggcatcg ccgttgccgg caaggcggcc
     2461 attgtggcgc gcgatgagac cgaaaccggc gagcgcatgc tgctcaatct aggacatacc
     2521 ttcgggcacg cgctcgaagc ctgggccggc tattccgata agcttttgca cggcgaggca
     2581 gttgcgatcg gcatcacgct ggcgttcgag ctttcgcacg aactgggctt cagcgatgct
     2641 gagagcgccg agcgggtgcg cacccacctg gcgagcgccg gattgccgac gcgcatcaaa
     2701 gatattgccg gcaatgcctg gcctgaggcc gacagcttgc tgcgcctgat ggcccaggac
     2761 aagaaagttc gcgccggcca gctcacgctg atcctcgcgc gcggcatcgg caaggccttc
     2821 gccacccggg aggtcgacac ggcaacggtg cgcgatttcc tggcgcggcg gatcggctag
     2881 tctgcccgcg gcagccgccc ggcgcgccgc ttgcgcgcgc gaagacgggt ccaaagcccc
     2941 ttaagagagg cgcaacggca actatgttat cgtcgcggcg gaggtctttt gggcggccct
     3001 ttgaggcagc ctcccggccg gccgcacgcc gccgcaaacg acgttagaga aagccatgtc
     3061 gctcgatatt gccttgatcc tgacagccat cgccgtgctc ttgctgttgt cggcattttt
     3121 caacggcagc gaaacggcgc tgacggcggt ctcgcgcgcc cggatgcacg ctctggagca
     3181 ggaaggcaat tccagggccc ggctcgtcaa tcatctgctc aaagagcccg agcgcctgat
     3241 tggcgtcgtc ctgcttggca ataccctcgt cgacattttg gccgcctcgc ttgcctccgg
     3301 cctggccgtg attctcatcg gcccggccgg ggtcgcctat gcgaccgcgg tcatgaccat
     3361 tctgatcgtg attttcgccg ccgttctgcc caagacgtac gccttcgcct tcgccgatcg
     3421 gctggcgctg ttcgtggcgc ccctgatgcg cgtgatgatt gcggtgctca atccggcaac
     3481 ccgcgccatc caggtgattg tgcgcatgct cctgaagctc acgcccacga gtcgtgacga
     3541 tgaggccaac atcctggaag cgcatgcgga gattcgcggc accatcgagc tgcagaagat
     3601 cgagggtgcg gtggccaagc gcgatgctgc catgctcggg ggcgtgctcg atctcaagga
     3661 cctgaaagtc ctcgacatca tggtgcaccg gaccaaaatg cagaccatca acatcagcga
     3721 tgccccggaa aaagtgatcg acgaggtgct caaaagtcag ttcacgcgcg tgccgctgtg
     3781 gaaggacgaa ccggagaaca tcgtcggcgt cctgcatacc aaggatctgc tgctggcatt
     3841 gtcacgtgcg ggctgggagg tggccaagat cgacattgct gcggtcgccg cccagccgtg
     3901 gtttgtgccg gacaccacca gcgttgccaa tcagttgaac gagttcttgc gccgcaaggc
     3961 acagctcgcc ctcgtggtcg acgagtatgg agaggtgcag gggctgatca cacttgagga
     4021 tattctggag gagatcgtcg gccagatcgc cgacgagcac gacgtgcagg acgccgccat
     4081 ccgcttgcag gccgacggct cggtcaatgt cgatggtacg gtggccgtac gtgatctcaa
     4141 tcgtcacatg gattgggatc tgccggacga agaggccacc accatcgccg gcctcgttat
     4201 ccacgaggcg cagagcattc ccgagccagg gcaggcattc acgttttacg gctatcgctt
     4261 cgagatcctg cgcaagaacc gcaacaagat cacggcgatc agggttcggc cggtcacggc
     4321 caagcagcgg cgtccgcgct cgagcctcga gagcgacgcc atggacgccg gctgatggag
     4381 accaaacgtc agggtgaagc gcgtagcggc gccatggccg gcaagggtgg cgggttgggg
     4441 gctgcacgcg cctcggccaa caatcgcgcg caggccgtct cgatgcgggc ctcgatcagc
     4501 gcgcgggcct cggcgcgcgc gagacccggc ggcaccggct cgaggatctc gaccacgatg
     4561 gtgcccggat agcgcatcag gctgcgccgc ggccagaaca ggccggaatt gagggcgagg
     4621 gggacgcagg gcagacccaa gccttcgtag agggcgatga agcctggctt gtagtccggc
     4681 ggcgcgccgg gaggccggcg ggtcccttcg gggaagatga caatttcgcg ggcggcggcg
     4741 gcccggttgc gggcatcgcg gatcatgctc ttgagcgcgc cggggccctt gtcccgcttc
     4801 acgaaaatat gctggaactt gtggctgaac cagccgtaga gcggtatcag cccgagctcg
     4861 gccttcatga ccatggcggg atcgcgaaac accgggatca ggccgaaggt atcccaggca
     4921 ctttgatgtt tggccgccac caggcaagcg ccgcggggca gtttttcgcg gccgcgcact
     4981 tcgaccttgg tgccgcagat gattttgagc caccaaaggc tggcaagccc atgcagccgc
     5041 agcccttgca tggcccagcg ccgcggcgcc agcagcagcc agctgccaaa cagcaaaaac
     5101 accgccgtat tcagatagaa caccgcgacg aacaggagcg aacgaagcat ggggcgcgag
     5161 ctgccgtgtc aaatgcgctg ggctgcagcc ggccgtgcac cggcgagggc gtcactatcc
     5221 cactggcgca gcaatcgcgc gacgccaaag cgcgcggccg agggcagaaa cttgaggtat
     5281 tcggtgaaca acaaacgcat gctgcccgcg tgcatccacc agcgttcggc gcggaaggtg
     5341 c
//
LOCUS       DASTMT010000523         4949 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_6386380, whole genome shotgun sequence.
ACCESSION   DASTMT010000523 DASTMT010000000
VERSION     DASTMT010000523.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4949)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4949)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4949
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6386380"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..573
                     /locus_tag="VFR00_12030"
     CDS             <1..573
                     /locus_tag="VFR00_12030"
                     /inference="COORDINATES: protein motif:HMM:NF042901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0160041.1"
                     /translation="PGASAIHHLVDEDVRNAPALADVVARFRGADVYVAHNCEFERSF
                     FAAQGIELGPWICTYKCALRVWPHFDGHSNQELRYALGRATPFPGVERGSISPHRAAF
                     DVIVTAAIFEELIKHARWSQMVQWSAEPALHTRFHFGKYRGKAYAEIAERDAEYLVWI
                     IEKSELEEGIKFSAKYWLDASSRASEPSPE"
     gene            complement(672..2318)
                     /locus_tag="VFR00_12035"
     CDS             complement(672..2318)
                     /locus_tag="VFR00_12035"
                     /inference="COORDINATES: protein motif:HMM:NF019430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HEU0160042.1"
                     /translation="MVRGTAAAIVMVGLAGGGLALWSHAAAARGGHFGGGFGGGGMHF
                     GGSHFGGGGMRFGGGHFGGGGMRFGGAPFGGMRFGGGMRFGGAPFGGMRFGGMAGPRF
                     SPRFPGGPFPGTRFGGMGARGAQVFSNGNRALARPNPGGLTAQRGFAASRLTGANTRF
                     TGSNGLRAATAGAAGARLWHGQAGAANARAFLHQGGFRHNAFASKVAWNTWGFRRHGC
                     CGWFGKIFWPFVVGDVFTAVLWPWPWYEPFWGYGGDYALSNVLWVAPAATPTSYSNMP
                     IYDIYGTGAGRAANAPAGGGAVADTPAEADLTQACAELAPGVADLPISDIERTVNPTG
                     DQSAALNALKTAWGGAIKLIKTSCSGEVPLTPVRRLDVVEERLKAVLRALQLVSAPLE
                     NFHNSLSQAQQHSFDSMTAAARERNEATPGPAGRLTALCSERATTFSRLPLDKIVDAI
                     RPTEAQRLALDALKSASARASEGLNASCPAQAPQSLPDRLRTVEQRLAAMISALQTVR
                     PALIDFYRSLSDEQKARFNLMGQTLTQGSIDQDGRQDRAN"
     gene            complement(2668..3642)
                     /locus_tag="VFR00_12040"
     CDS             complement(2668..3642)
                     /locus_tag="VFR00_12040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017486970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEU0160043.1"
                     /translation="MRAVIRLRLIALLLSAGMLPAQAQDYPSRTITVIVPFPPGGASD
                     VVARIVTDQMSRILKQPMVIENVAGAGGTLGSGRAAAAAGDGYTLLAAAMGSHVAAPV
                     LTPNLKYDPSTSFVPIGITAHSPAIVIARKDFPARDLNEFVAFLQQKNDALKQAHGGI
                     GASSHMACLLFTKAIGATPTLVAYRGSAPALNDLIGGHVDFLCEQSVAVAESVLGGTV
                     KAYATSGDKRSPTLPNVPTARELGVPFEMSIWAGLFAPKETPAEVVSKLAAALDQALE
                     EPMVRDRLIQLGASIPDKAERTPAAFDRFVRAEMARWAPLLAAVPGKP"
     gene            complement(3671..4687)
                     /locus_tag="VFR00_12045"
     CDS             complement(3671..4687)
                     /locus_tag="VFR00_12045"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU0160044.1"
                     /translation="MDQNPLRVASIGIGWWSDVLADAVKRSGKLKIVSCYSRSPDKRT
                     AFAERYACRAAASYEAVLADPEVEAILNTTPNDTHLVTTTAAAAAGKHIFLDKPIANS
                     VADGRAITAACRRAGVVLALGYQRRREGHFRWIKQQIEAGQFGRLVNAEANISRDRLG
                     KVDLGSWRYQAEGMPGGVMLQIGIHYTDVLEYLLGPIKAVRGELARLVLPGDNPDVAS
                     LLLQHENGALSTLNASYASASEYYLMNIYGKDATAYYDLHTGLRLLRRGAAEPVAVAT
                     AKTDTLVEQLEEFAAAVRTGTGIEVGGDYATRSLAVVRAGILAAQRGRAVEVAEVLRD
                     GAEV"
     gene            complement(4742..>4949)
                     /locus_tag="VFR00_12050"
     CDS             complement(4742..>4949)
                     /locus_tag="VFR00_12050"
                     /inference="COORDINATES: protein motif:HMM:NF015862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HEU0160045.1"
                     /translation="AVGQDLDVTHAIGLALAAGPERWTAMEGGVPILIDGQCVGGVGV
                     SGGDWETDLRIAKASVEAIGAGWA"
BASE COUNT          803 a         1667 c         1662 g          817 t
ORIGIN      
        1 ccgggggcct ccgccatcca tcatctcgtc gatgaggacg ttcgcaacgc gccggccttg
       61 gccgacgtgg tggctcgctt ccgaggggcg gacgtctatg tcgcgcataa ttgcgaattc
      121 gagcgcagtt tctttgccgc ccagggcatc gagctgggtc cctggatttg cacctacaaa
      181 tgcgccttgc gcgtgtggcc tcacttcgac ggtcatagca atcaagagct gcgctatgcg
      241 ctcggccggg ccacgccatt ccccggcgtt gaacgcggct cgatctctcc ccaccgcgcc
      301 gcctttgacg tgatcgttac cgcggcgatc ttcgaggaac tgatcaagca cgcacgctgg
      361 agccagatgg tgcaatggtc cgccgagccc gcattgcaca cgcgcttcca ctttggaaaa
      421 taccggggca aggcctacgc cgagattgcc gagcgggatg ccgagtacct cgtctggatc
      481 atcgaaaaat cggagctcga ggagggcatc aaattcagcg ccaagtattg gctcgatgcc
      541 tcctcgcgtg cctccgagcc gagccccgag taggctaggg cgcggttgcc gccgcccgct
      601 gcgcctagcg gcgtgcgcac tatcaattga gcccgcgccc gtttacgcct cgcgcgcgcc
      661 ggcgagagag ttcagttggc gcggtcttgc cgaccgtcct ggtcgatgct cccctgcgtc
      721 aaggtttggc ccatcaggtt gaaccgcgcc ttctgctcat cgctcaaaga gcgatagaag
      781 tcgatcaggg ctggccgcac ggtttgcaaa gcggagatca tggcggcaag ccgctgctcg
      841 acggtgcgca agcggtccgg caagctttgc ggggcttgcg ccgggcacga cgcgttgagc
      901 ccttcgctcg ccctggcgga ggccgatttg agcgcatcca aggcaagacg ttgcgcttcg
      961 gtggggcgga tcgcgtcgac gattttgtcg agagggagcc gggagaaggt ggtcgcccgc
     1021 tcgctgcaca aggccgtcaa gcgcccggcc ggacctggcg tggcttcgtt gcgctcgcgt
     1081 gcggccgccg tcatgctgtc gaagctgtgt tgttgggcct ggctcagcga attgtggaag
     1141 ttttcaagcg gtgcgctcac gagttgcaag gctcgcagca ccgccttcag gcgctcttca
     1201 accacgtcga gccgccgcac cggcgtgagc ggcacttcgc cggagcagga ggtcttgatg
     1261 agcttgatgg caccgcccca ggccgtcttc agcgcgttga gagccgcgct ctggtcgccc
     1321 gtcgggttga cggtgcgctc gatgtcgctg atcggcagat cggcgacgcc gggcgccagc
     1381 tcggcgcagg cttgggtcaa gtcggcctcg gccggcgtgt cggccaccgc accgcccccg
     1441 gcaggggcgt tggcggcgcg cccggcgccg gtgccataaa tgtcgtaaat gggcatgttg
     1501 ctgtaggagg ttggcgttgc ggcgggggcg acccacaaca cattgctcag cgcatagtca
     1561 ccgccatagc cccagaacgg ctcgtaccac ggccacggcc aaagcacggc ggtgaagacg
     1621 tcgccgacca cgaagggcca aaagatcttg ccaaaccagc cgcagcaacc gtggcgtcgg
     1681 aagccccagg tgttccaggc gaccttgctg gcaaaggcgt tgtgcctgaa cccaccctga
     1741 tgcaagaagg cgcgcgcgtt ggcggcaccg gcttgcccat gccaaagccg ggcgccggcg
     1801 gcgccggcgg tggcggctcg caaaccgttg ctaccggtga agcgcgtatt ggcgccagtg
     1861 aggcggctcg cggcgaagcc tcgttgcgcc gttagcccgc ccgggttggg ccgggcgagg
     1921 gcccggttgc cgttggaaaa gacctgggcg ccgcgcgccc ccatgccgcc aaagcgcgtg
     1981 ccggggaagg gcccgccggg aaagcgcggg gaaaaccgtg ggccggccat cccgccaaag
     2041 cgcataccgc cgaacggtgc cccgccgaag cgcataccgc cgccgaagcg catgccgcca
     2101 aaaggcgcgc caccaaaccg catgccgccg ccaccgaaat ggccgccacc aaagcgcatg
     2161 ccgccgccac cgaaatggct gccgccaaag tgcatgccgc caccgccaaa gccgccgccg
     2221 aaatgtccac cgcgcgccgc cgcagcatgg ctccagagcg caagcccgcc gccggcgagg
     2281 cccaccatga caatggctgc cgctgttcct ctcaccatgg catcctcccc tggtattccc
     2341 acgctttgcg cagcgcctgc cgcgggagcc ggccaggtgt cagggcagca ccgggggtgc
     2401 tgctgctcgc gcatcgagag cccgcgagag gcccgtcgca cctggcctcc tctgggtgcc
     2461 aggctgcgcc aggcaaagca cgctgcctat cggtactaac cctttgcccg tactggctgg
     2521 cagccgagtc gcgacctcag gctccagacc ccgatcgcag ggcgagccgc gtaagcttgg
     2581 caattcgggc aaggccgggc gccctcggtt gccaagggct gggcggcccg tcgggtaagt
     2641 gccgcgccgg cgccgtgccc tgcgcgctta gggtttgccg ggcacggcgg cgagcagggg
     2701 tgcccagcgc gccatttcgg cgcgaacaaa gcggtcgaag gcggcgggtg tccgctcggc
     2761 cttgtcggga atgctggccc caagttggat caagcggtcg cgcaccatgg gctcctcgag
     2821 ggcctgatcc agcgcagcgg cgagtttgct caccacctcg gccggggtct ccttgggagc
     2881 gaacaggccg gcccagatgc tcatctcgaa ggggaccccc aactccctcg cggtcggcac
     2941 attgggcagc gtcggggagc gtttgtcgcc ggaggtggcg taggccttga cggtgccgcc
     3001 gagaaccgat tctgccactg ccaccgactg ctcgcagagg aagtcgacat gcccgccaat
     3061 caggtcattg agcgcgggcg cagagccgcg gtaggcaacg agcgtcggcg tggcgccgat
     3121 ggccttcgtg aacaggaggc aggccatgtg tgaggaggca ccaatgccgc cgtgcgcctg
     3181 cttcagcgca tcgttctttt gctgcagaaa cgcgacgaac tcgttgagat cccgggccgg
     3241 gaagtccttg cgcgcaatga cgattgccgg cgaatgggcg gtgatgccga tcggcacgaa
     3301 gctcgtcgag ggatcatatt tgagattggg tgtgagcacg ggtgcggcga cgtgcgagcc
     3361 catcgccgcg gccaacaggg tgtagccgtc gcctgccgcg gccgcggcgc ggccgcttcc
     3421 cagcgtgccg ccagcgccag cgacgttttc gatcaccatc ggctgcttga ggatcctcga
     3481 catctgatcg gtgacaatgc gcgccacgac gtcgctcgcg cctcccggcg ggaaggggac
     3541 gatcacggtg atcgtgcgcg aggggtagtc ctgggcctgg gccggcagca tgccggcgct
     3601 cagcagcaag gcaatgaggc ggaggcggat gacggctcgc atgctgggct cctcgcgggg
     3661 ttgattacgg ctaaacctcg gcgccatccc gcaacacctc ggcaacctct accgcgcgcc
     3721 cccgctgcgc ggcgagaatc cccgcccgca ccacggccag cgagcgtgta gcgtagtcgc
     3781 cgccgacttc gatcccggta cccgtgcgga ccgcggcggc aaattcctcc aactgctcca
     3841 ccaaagtgtc cgtcttcgcg gtcgcaaccg cgaccggttc ggctgccccg cgccgcagca
     3901 ggcgcaagcc agtgtgcagg tcgtaatagg cggtggcgtc cttgccgtag atattcatca
     3961 ggtagtattc cgatgccgag gcgtagctgg cattcaaagt ggaaagcgcg ccgttctcgt
     4021 gctgtagcag caggctggcc acatcgggat tgtcacccgg cagcaccaag cgggcgagct
     4081 ctccgcgcac cgccttgatg ggcccaagca ggtattccag cacgtcggtg tagtggatgc
     4141 cgatctgaag catcacgccg ccaggcatgc cctcggcttg gtaacgccag gagccaagat
     4201 cgaccttacc aagccggtcg cggctgatat tcgcctcggc attgaccagc ctgccaaact
     4261 gcccagcctc gatctgctgt ttgatccagc gaaaatggcc ttcgcgtcga cgctggtagc
     4321 cgagtgccag caccacgccg gcgcgccggc aggcggcggt aatggcgcgg ccgtcggcca
     4381 cgctgttggc gatgggtttg tcgaggaaga tgtgcttgcc ggcggccgcc gcggctgtgg
     4441 tcgttacaag gtgcgtatca ttcggtgtgg tgttgagaat ggcctcgacc tcggggtcgg
     4501 ccagcaccgc ttcgtagctt gccgccgcgc ggcaggcgta tctctcggcg aaggctgtgc
     4561 gcttgtccgg cgaccgcgag tagcagctca cgatcttgag cttgcccgag cgtttaaccg
     4621 cgtcggccaa cacgtccgac caccaaccga tgccgatgga ggcgacccgc aacgggtttt
     4681 gatccatgtt gatcccgccg gcagcgacct caccgttcgc cgcgcggccc cttgtgtttt
     4741 gctaagccca gccagccccg atggcttcga cgctggcctt ggcgatgcgc aggtccgttt
     4801 cccaatcgcc gccgctgacc cccacgccgc ccacgcactg accgtcgatc aggatgggga
     4861 cgccaccttc catggccgtc catctttccg gccccgcggc cagcgccagg ccaatggcgt
     4921 gcgtgacgtc gaggtcctgc ccgacggcg
//
LOCUS       DASTMT010000524         2115 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_630674, whole genome shotgun sequence.
ACCESSION   DASTMT010000524 DASTMT010000000
VERSION     DASTMT010000524.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2115
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_630674"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            41..1150
                     /locus_tag="VFR00_12055"
     CDS             41..1150
                     /locus_tag="VFR00_12055"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019169801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009008 - DNA-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HEU0160046.1"
                     /translation="MGKKPAPAEGGLSLDRIIHGDCIAAMNVLPEGCADLVFADPPYN
                     LQINNELLRPNNTRVDGVDDDWDKFGSFAAYDDFTAAWLGACRRLLKPEGALWVIGSY
                     HNIFRVGTILQDLGFWVLNDVVWRKTNPMPNFRGRRFTNAHETLVWVSMGPKARYTFN
                     YEAMKALNDELQMRSDWLFPICSGPERLKQDGGRKTHPTQKPEALLHRIILASSNKGD
                     VILDPFLGSGTTAAVAKRLGRKFIGIEREQAYADAARARLDLVTPLKGASVETVRGKR
                     AEARIPFGALVEIGLISPGTVLYDARARHEAEVRADGSIASAGAQGSIHKIGAHVQGA
                     SACNGWTFWHYDADGTLKPIDALREVARRQLEPEA"
     gene            complement(1092..>2115)
                     /locus_tag="VFR00_12060"
     CDS             complement(1092..>2115)
                     /locus_tag="VFR00_12060"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020186741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000701 - purine-specific mismatch base
                     pair DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEU0160047.1"
                     /translation="PYLVWLSEIMLQQTTVKAVLPRYAQFLRRWPDVRALAAAELGEV
                     LAAWAGLGYYARARNLHACAREVVARFGGRFPATEEELRTLPGIGDYTAAAIAAIAFG
                     ARATPIDGNIERVVARLFAVSTPLPAAKAEIRALAVALTPAERAGDFAQAMMDLGATL
                     CSPKRPACGLCPLMSGCAGHAQGLAEFLPYRAEKPERPTRRGHAFVALRADGAVLLRE
                     RPLKGLLGGMLETPSSPWAEGVWQRPQLDHAPLSAEWRELPGLVEHTFTHFHLELAVY
                     FAEVATGAKLAKAAQPERCRWVHERDLHGAALPSVMRKVLAHALEAKPPARAAAGPPR
                     ARRRSA"
BASE COUNT          361 a          741 c          695 g          318 t
ORIGIN      
        1 ctcaatttgc ccgcctttgt ggataggaat gggcggggac atggggaaga aaccggcgcc
       61 ggccgagggc ggcttatctc tcgaccgcat catccatggc gattgcatcg cggcgatgaa
      121 cgtgcttccc gagggttgcg ccgatctcgt ctttgccgac ccgccctaca atctgcagat
      181 caacaatgag ctgctcaggc cgaacaacac gcgcgtcgat ggcgtcgacg acgattggga
      241 caagttcggc agcttcgcag cctatgacga cttcaccgcg gcgtggctcg gggcctgccg
      301 ccgcctgctg aagccggaag gcgcgctctg ggtgatcggc agttatcaca acatcttccg
      361 cgtcggcacc atcctccagg acctcggctt ctgggtgctg aacgacgtgg tgtggcgcaa
      421 gaccaacccc atgcccaatt tcagaggccg ccgcttcacc aacgcgcacg agaccttggt
      481 gtgggtctcc atggggccga aggcccgcta caccttcaac tacgaagcga tgaaggcgct
      541 gaacgacgag ctgcagatgc gctcggactg gctgttcccg atctgctcgg ggccggagcg
      601 gctcaagcaa gacggcgggc gcaagaccca tccgacgcag aagccggagg ccctgctcca
      661 ccgcatcatc ttggcttcga gcaacaaggg cgacgtcatc ctcgatcctt tcctcggctc
      721 aggcaccacg gcagcagtgg ccaagcgcct tggccgcaag ttcatcggca tcgagcgcga
      781 gcaggcttac gccgacgctg cgcgggcgcg gcttgatctc gtgacgccgc tcaagggcgc
      841 ctcggtcgag accgtgcgcg gcaagagggc tgaggcgcgc atccccttcg gcgccctggt
      901 cgagatcggg ctgatctctc ccggcaccgt gctctatgac gcccgcgccc gccacgaggc
      961 agaagtgcgc gccgacggca gcatcgcctc ggccggcgcc caaggctcga tccacaagat
     1021 cggcgcgcat gtgcagggcg cctccgcctg caacggctgg accttctggc attacgacgc
     1081 cgacggcacg ctcaagccga tcgacgcctt gcgcgaggtg gcccggcggc agctcgagcc
     1141 ggaggcttag cctccaaggc gtgcgcgagt actttccgca tcacgctcgg caacgccgcg
     1201 ccgtgcaggt cacgctcatg cacccagcgg cagcgctcag gctgggcggc cttggcaagc
     1261 ttcgcgccgg ttgcgacctc ggcaaaataa acggcaagct caaggtgaaa atgggtgaag
     1321 gtgtgctcga cgaggcccgg cagctcgcgc cattccgcgc ttagcggcgc atgatcgagc
     1381 tgcggtctct gccaaacgcc ctcggcccag ggcgaggacg gcgtctccag catgccgccg
     1441 agcaggccct tcagcggccg ctcccgcagc agcaccgccc cgtccgcccg caacgccacg
     1501 aaggcatggc cgcggcgtgt cggccgttcc ggcttctccg cgcgataggg cagaaactcg
     1561 gccaggcctt gcgcgtggcc ggcgcaccct gacatgagcg ggcagaggcc acaggcgggg
     1621 cgcttcggac tgcacagcgt cgcgcccaaa tccatcatgg cttgggcaaa gtcccctgcc
     1681 cgctccgccg gcgtcagcgc caccgcaagc gccctaatct cggccttggc cgcaggcagc
     1741 ggcgtggaga cggcgaagag cctcgccacc acgcgctcga tattgccgtc gatcggggtt
     1801 gcgcgcgcgc cgaaggcgat ggccgctatg gccgctgcgg tatagtcgcc gatgcctgga
     1861 agcgtgcgga gctcttcctc ggtcgcgggg aacctgccgc cgaagcgcgc caccacctcc
     1921 cgggcgcaag cgtgcaggtt ccgcgccctg gcgtaatagc caagcccggc ccaggccgcg
     1981 agcacctcgc caagctcagc cgccgccagc gcccgcacgt cgggccagcg gcggaggaat
     2041 tgggcgtagc gcggcagcac cgccttgacc gtggtctgct gcagcatgat ctcgctgagc
     2101 cacacgagat aaggg
//
LOCUS       DASTMT010000525         3243 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_958119, whole genome shotgun sequence.
ACCESSION   DASTMT010000525 DASTMT010000000
VERSION     DASTMT010000525.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3243)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3243)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3243
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_958119"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..788
                     /locus_tag="VFR00_12065"
     CDS             <1..788
                     /locus_tag="VFR00_12065"
                     /inference="COORDINATES: protein motif:HMM:NF017026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEU0160048.1"
                     /translation="AGERTDITEDAFFGGSLRISQPAAGYRAGLDAVLLAAAAPVRDG
                     RREQVLDVGAGVGVVGLSVARRAAQACVTLIERETTQAELCRLNIERNGLTERARLLT
                     GDIGKPLGEQPMLKSLAGSFDHVLANPPYQVEGTATLAALPAKAAANAMRREGLQQWL
                     RFMAAMAQPGGTATMIHRADSLDQVLAAFSGRFGRLLVYPIFPRQGASAHRVLVQGIK
                     GSRAPLQLRAGLILHATGHAFRPEVDAILRGGAALDLGAASAS"
     gene            913..1758
                     /locus_tag="VFR00_12070"
     CDS             913..1758
                     /locus_tag="VFR00_12070"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HEU0160049.1"
                     /translation="MLFFNRGPIVPVLRFTGAIGMVTPLRPGLALASLAGAIEKAFNA
                     SKLPSVAISINSPGGSPVQSHLIFSRIRQLAAEKEKRVYVFCEDIAASGGYFLALAGD
                     EIYADPSSIVGSIGVISAGFGLDKFIQRFDVERRVHAVGKDKGALDPFQPERPEDVRR
                     LKELQRDVHDTFIGIVKARRGGRLRGPEEEMFSGSFWSARKALEYGLIDGIADLRSKM
                     RALHGDKVRLKAVPMAPGGLLTRLRRLPGLAAHGGDGFAFPASLADDVISAVEARALW
                     SRFGL"
     gene            1780..1992
                     /locus_tag="VFR00_12075"
     CDS             1780..1992
                     /locus_tag="VFR00_12075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0160050.1"
                     /translation="MPQLIVVALIGAGVYAAYRWVRQASEAITAELNRAEDDLQRRTA
                     GGRVEKDLGRLEYDAATGVYRPQKRA"
     gene            2213..3184
                     /locus_tag="VFR00_12080"
     CDS             2213..3184
                     /locus_tag="VFR00_12080"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HEU0160051.1"
                     /translation="MQKKAAKPADGKLAKASPGLRPTASFQDLILTLQQYWGEQGCAI
                     LQPYDMEVGAGTFHPATTLRALGPKPWNAAYVQPSRRPKDGRYGENPNRLQHYYQFQV
                     IMKPSPVDFLDLYLESLARIGIDPKVHDIRFVEDDWESPTLGAWGLGWEVWCDGMEVT
                     QFTYFQQVGGFDCNPVSGEITYGLERLAMYVQGVDRVFDLNFNGREDERKLSYGDVFL
                     QAEQEYSRFNFEYADTDILLRHFRDAEKECKALLAAGAAAAGANDTRHRLALPAYDQC
                     IKASHIFNLLDARGVISVTERQSYILRVRELAKACCAAWLRTEGGGQ"
BASE COUNT          538 a         1055 c         1064 g          586 t
ORIGIN      
        1 cggccggcga gaggacggac atcaccgagg acgcattttt tggcggttcc ttgcgcattt
       61 cgcagcccgc agctggatat cgcgcggggc tcgatgccgt gttgttggcg gcggccgcgc
      121 cggtgcgcga tggtcgccgc gagcaggttc tcgacgtcgg cgccggcgtc ggggtggtcg
      181 gcctttcggt ggcgcggcgc gcggcgcagg cttgtgtcac cctgatcgag cgcgagacca
      241 cccaggccga gctctgccgc ctcaacatcg agcgcaacgg cctaactgag cgcgcgcgcc
      301 tgcttacagg cgacatcggc aaaccccttg gcgaacagcc catgctcaag tcgctcgccg
      361 gaagcttcga ccacgtgctc gccaatcctc cttatcaggt cgaaggcacc gctaccctgg
      421 cagcgctgcc ggccaaggcc gccgccaatg ccatgcggcg cgagggtctg caacagtggc
      481 tgcggttcat ggccgccatg gcgcagccgg gcggcacggc caccatgatc catcgcgccg
      541 acagtctcga ccaggtactg gcggcctttt ccggacgctt cgggcgctta ctcgtctatc
      601 ccatctttcc ccgtcagggg gccagcgcac accgcgtgct ggtgcagggc atcaagggca
      661 gtcgagcccc gttgcagctc agggccgggc tgattttaca cgcgacgggc cacgcctttc
      721 gacccgaggt cgatgccatc ctgcgcggcg gcgccgccct tgaccttggc gccgcgtctg
      781 ctagctgatg cgcgtccgct gcggcaaagt ctttagccgc atcctcccgg ccggtggctc
      841 ggcagtaaaa accggcgccg taaggcttac gtgtcagcgt ccatttgccc atcctagccc
      901 gcgaggcagc cgatgctctt cttcaaccgt ggcccgattg ttccggttct gcgctttacc
      961 ggcgccatcg gcatggtgac gccgctacga ccaggacttg ccctggcatc gctggccggc
     1021 gccatagaaa aggccttcaa cgcctcgaaa ttgccgagcg tggcaattag tatcaactcc
     1081 cccggcggct cgccggtgca gtcgcacttg atttttagcc gcatccgcca gcttgccgcc
     1141 gaaaaggaaa agcgggttta cgtattttgc gaggacatcg ccgcctcggg cggctatttc
     1201 ctggccctcg ccggcgacga aatctatgcg gatccctcct cgatcgtggg ctcgatcggc
     1261 gtcatctccg cgggctttgg tctcgacaag tttatccagc gcttcgacgt cgagcggcgc
     1321 gtgcatgcgg tcggcaagga caagggcgcg ctcgacccgt tccaacccga gcgaccggag
     1381 gacgtgcgcc ggctgaaaga gctgcagcgc gacgtgcacg ataccttcat cggtatcgtc
     1441 aaggcgcggc gcgggggcag gctcaggggc cctgaggagg aaatgttctc gggcagcttc
     1501 tggtcggcac gcaaggccct tgagtatggg ctgattgatg gtatcgccga tctgcgcagc
     1561 aagatgcggg cactgcacgg cgacaaggtg cggctcaagg ccgtgccgat ggcgcccggc
     1621 ggcctgctca cccggctgcg gcggttgccg ggccttgccg cccacggcgg cgacggattt
     1681 gctttcccgg cgtcgttggc cgatgatgtc atctccgccg tcgaggcgcg ggccttgtgg
     1741 tcgcgcttcg gcctgtagtc gtcagcggga ggcacgccaa tgccccagct catcgttgtc
     1801 gccctcatcg gcgccggagt ttatgcggcc tatcgttggg tgcggcaggc gtcggaggcg
     1861 attacggccg aattgaaccg ggccgaagac gaccttcagc ggcggacggc gggcggccgc
     1921 gtcgagaagg acttgggcag gctcgaatat gatgcggcga ccggcgtcta tcggccgcaa
     1981 aaacgcgcct gagcagcttg gccgtgatgg cgacggaccc ggccgggcgc gggcttttca
     2041 tcaccaggcg ttggggacgc gggcctgccc cgggggggtt tggcttcaag gtgacccgag
     2101 cggcctcggt ctataactgg gctcgccttg gcggctgcgg ctccgtgaaa tccaggtgac
     2161 cgatggcgcc atttgcgtat agtgcgcccg ccgcagcact cagaccacac cgatgcaaaa
     2221 gaaagccgcc aagccggcag acgggaaact tgccaaggcc tcgccgggcc tgcgtcccac
     2281 ggcatctttc caggacctta tcctaacctt gcagcaatac tggggcgagc agggctgcgc
     2341 tatcctgcaa ccctacgaca tggaggtggg tgcgggcacc tttcaccccg ccaccacact
     2401 gcgcgcgctc ggaccaaaac cgtggaacgc cgcctacgtg cagccctcgc gccggcccaa
     2461 ggatgggcgc tacggcgaga accccaatcg gctgcagcac tactaccagt tccaggtcat
     2521 catgaaaccg tcgccggtcg actttctcga cctttatttg gagagcctcg cgcgcatcgg
     2581 catcgatccc aaggtgcacg acattcgctt cgtcgaggat gattgggaga gcccgacgct
     2641 tggcgcctgg ggattgggtt gggaagtgtg gtgcgatggc atggaggtga cgcagttcac
     2701 ctacttccaa caggttggcg gctttgactg caacccggtc tcgggcgaga tcacctacgg
     2761 cctcgaacgc ctggcgatgt acgtccaagg cgtcgatcgc gtgttcgacc tcaacttcaa
     2821 tggccgcgaa gacgaacgca agttgagcta tggcgatgtg tttctgcagg ccgaacagga
     2881 atactcacgt tttaatttcg agtacgccga tacggacatc ctcttgcggc actttcgcga
     2941 tgccgaaaag gagtgcaagg cgctgctggc cgccggcgct gccgccgccg gagccaacga
     3001 tacgcgccat cggttggcgc tgcccgccta cgatcagtgc atcaaggcaa gtcacatctt
     3061 caatctgctg gacgcgcgcg gcgtcatctc tgtcaccgag cgccagagct acattctgcg
     3121 cgtgcgcgag ctggctaagg cctgctgtgc ggcctggctc aggaccgaag ggggcggcca
     3181 atgagggtgg ggaggccgcg gcggctctac cgcgcccgac gcaaggcgcg cgtactccag
     3241 caa
//
LOCUS       DASTMT010000526         5732 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_4418693, whole genome shotgun sequence.
ACCESSION   DASTMT010000526 DASTMT010000000
VERSION     DASTMT010000526.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 5732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5732
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4418693"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1174
                     /locus_tag="VFR00_12085"
     CDS             <1..1174
                     /locus_tag="VFR00_12085"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEU0160052.1"
                     /translation="QFEAGPSCTESLAWLGAEVVKIEALGTGDPGRRLHPGRIQDDPW
                     YFHQFNANKKSVTLNLKSAQGLAIVRALLAKADVTVENMAPGTIERLGLSYAEVKAIN
                     PGIIYCRVKGFGAGSPYEKNLAFDMIAQAAGGTISVTGDADRAPVKPGTSLGDTGTGM
                     LMAISILGALYQRAKTGQGRLLEVAMQDAMLHYMRVPFSRTQLSGKAVQRDGSSRSTP
                     GGLTPSALYPCDPGGPNDYVYVFCSRANPEHWQRLLKVIGREDLSGDSRYDTQRARSE
                     RGAEVDAIISDWTRRRSKQEAMRLFGQAGVPAGAVFDTLELLNEPTFVERGIMQTVEH
                     PTTGTVKMPTWPVRFDGAAPKLRPSPLLGEHVEDVLGSWLAMSTSDIAVLREEGIL"
     gene            1228..2049
                     /locus_tag="VFR00_12090"
     CDS             1228..2049
                     /locus_tag="VFR00_12090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018517175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU0160053.1"
                     /translation="MLSLKDKVVFISGAGSVGDDPDAKVWGNGKATAVLLARQGATIY
                     GVDVRKEAADVTKSIIEREGGTCATRGVDMTKAPEVEAAVADCMGRFGRIDVLVNNVG
                     GSAPGTAVSMSEEVWDQQIEHNLKTAFLASKYVLPIMERQGKGAIVNLASVAGLRMSP
                     DRAHIAYSTTKLGILAFSKSTAIAYAKKGIRCNTVIPGLMHTALVEHRLAKTIGANDV
                     EALVAKRNAQCPTGKMGDAWDIAHAVLFLASDEARYITAAEILVDGGLTATTGVG"
     gene            complement(2085..2492)
                     /locus_tag="VFR00_12095"
     CDS             complement(2085..2492)
                     /locus_tag="VFR00_12095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020608033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HEU0160054.1"
                     /translation="MRLEGGCYCGKVRYLAEGEPMMQAQCHCRECQYITGGAPNFFIA
                     MPVSGFRYTKEQPKAFRRSDLERPVTREFCAECGTHLATRPQGFPAVIVKVGTLDDPS
                     QFGTPKLAIYTIDKQAFHHIPEDLPNFERLPKR"
     gene            2709..3353
                     /locus_tag="VFR00_12100"
     CDS             2709..3353
                     /locus_tag="VFR00_12100"
                     /inference="COORDINATES: protein motif:HMM:NF014622.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEU0160055.1"
                     /translation="MTQDEPDCRIYLVVEAAPAAIEHLSAAAAVADIACCLILPASGA
                     PDAASRARPIVAAAQQAGIAALIAGDADLARALRADGVHLAPSPQILAAYTAARQMLG
                     REAIVGADAGVSRHDAMSLAEAGADYVAFGAPSHLKDRARARERRDALLAWWSEIFEV
                     AGVALDVEAADEAERLARAGADFVAVAVPAASAAPTGELLAKIGAAIGANVEAG"
     gene            3364..4434
                     /locus_tag="VFR00_12105"
     CDS             3364..4434
                     /locus_tag="VFR00_12105"
                     /inference="COORDINATES: protein motif:HMM:NF019843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HEU0160056.1"
                     /translation="MRSIFVLVLTLTLLAPSAHAETSSWTSQGDAAGKKGTAPAKAAK
                     PGAAKSATPAPSTSAAPAGDGVSSHAKGATQGEDPAYEAFDQGRYLTALELAVKAAEQ
                     GDPQAHTLVGRLYGEGYGVAKNSALAAKWYARGAELGDPEAMFAYAMMLVEGQGIDKD
                     PVAAARYFEAAAVHRHALANYNLALLFLRGEGKPENPYRAFMHMQFAAEAGVTAAQYD
                     LGTLYATGTGVEPNAFEAARWIGKAAAAGHPEAALEFGVILFRGHGIPVDEKRGVEMF
                     RAAAEKGLASAQDRLARCYVYGAGVEKNLVQGAKWYLIAKAGGLEDDGLEKVLTQLSS
                     ADRSKAQKAASEWRERAQVGMP"
     gene            complement(4464..5141)
                     /locus_tag="VFR00_12110"
     CDS             complement(4464..5141)
                     /locus_tag="VFR00_12110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020187858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEU0160057.1"
                     /translation="MATDVDKGLAPEARGERLKRRQILEGARHMFLAQGFDAASMGAI
                     AREAGVSKGTLYVYFKSKEELFEAIVEEQCRAEAEQIFTLDREAPIASELQRLGEELT
                     RFLCRPDGVPSLRTIIAIADRMPEIGAQFYASGPALGIGSLKRYLEDKIAQGGLEPHD
                     TEVAAAQFIDACVSTTFKPMLFNAAGPPTPARISHVVGMAVKAYLATYQNFKQREGAM
                     ARVTAAD"
     gene            5300..>5732
                     /locus_tag="VFR00_12115"
     CDS             5300..>5732
                     /locus_tag="VFR00_12115"
                     /inference="COORDINATES: protein motif:HMM:NF024923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HEU0160058.1"
                     /translation="MFEDQELKDNPMDRSPEGGEYAPLSQPAAAPSSGVAPRRRLVVL
                     AVLVGLALAAAGTTYGRYWLTQGRYLVSTDDAYVGAKSATLSPKVSGYIAEVAVEDNA
                     QVAAGDVIARIDPGDYQLAVANARDQIGVQQATIDRLGRQTT"
BASE COUNT          989 a         1918 c         1880 g          945 t
ORIGIN      
        1 ccagttcgaa gctggaccct cttgcacgga atcgctggcc tggctcggcg ccgaggtcgt
       61 caaaatcgag gccttgggca ccggcgaccc cggccggcgc ttgcaccccg gtcgtatcca
      121 ggacgatccc tggtattttc atcagttcaa cgccaacaag aagtcggtga ccttgaacct
      181 caagtcggcg cagggccttg ccatcgtgcg ggcgctgctc gccaaggccg acgtgacggt
      241 cgagaacatg gcgccgggca ccatcgagcg gctcggtctc agttacgccg aggtcaaggc
      301 gatcaatccg ggcatcatct attgtcgcgt caagggcttt ggcgccggca gcccgtacga
      361 gaaaaacctt gccttcgata tgatcgcgca ggccgcgggc ggtaccatca gcgtcaccgg
      421 cgatgccgac cgcgctcccg tcaagccggg aacgtccctc ggcgacacgg gcaccggcat
      481 gctgatggcc atcagcatcc tcggagcact ctatcagcgt gccaagaccg ggcaggggcg
      541 cctgctcgaa gtggccatgc aggacgccat gctccactac atgcgcgtcc ccttctcgcg
      601 cacgcaactg agcggcaaag ccgtgcagcg cgatggcagc agccgctcca cacccggtgg
      661 cctgacgccg tcggcgcttt acccctgcga tcccggtgga cccaatgact acgtctacgt
      721 cttctgcagc cgggcgaacc cggaacattg gcaacgcttg ctcaaggtca tcggacgcga
      781 ggatctgagc ggggatagcc gctacgacac gcaacgcgcg cgcagcgaac gtggggcgga
      841 ggtcgacgcc atcatcagcg attggacgcg ccgccgcagc aagcaagaag ccatgcgcct
      901 gtttggacaa gcgggggtac ccgccggcgc ggttttcgat accttggagt tgctgaacga
      961 acccactttc gtcgagcgcg gcattatgca gacggtcgag catcccacga ccgggacggt
     1021 caagatgccg acctggcccg tacgcttcga tggcgcagcg ccgaagttgc gaccctcgcc
     1081 actgctgggt gagcatgtcg aggacgtatt ggggtcgtgg cttgccatga gcaccagcga
     1141 catcgccgtg ctgcgcgagg aaggcatcct ctgagcccac ttcgcctggg ttgaccagcc
     1201 caccgccgcc tgaccgaggg aaacagcatg ctcagtctca aggacaaagt cgttttcatt
     1261 tccggtgccg gttcggtcgg cgacgatccc gatgccaagg tctggggcaa cggcaaggcg
     1321 accgccgtgt tgctggcgcg ccagggggcc acgatttatg gcgtcgacgt gcgcaaggag
     1381 gccgccgacg tcaccaagtc gatcatcgag cgggagggcg gcacctgcgc cacccgcggc
     1441 gtcgacatga ccaaggcgcc ggaggtcgaa gccgcggtcg ccgattgcat gggccggttt
     1501 gggcgcatcg acgtgctcgt caacaacgtc ggtggctcgg ccccgggcac cgcggtcagc
     1561 atgagcgaag aggtctggga ccagcagatc gagcacaacc tcaagacggc gtttttggcc
     1621 tcaaagtacg tgctgccgat catggaacgg cagggcaagg gcgccatcgt caatctcgcc
     1681 tccgtggcgg gtctgcgcat gtcgcccgat cgcgcgcaca tcgcctactc gacgaccaag
     1741 ctcggcatcc tggcgttttc caaatcgacc gcgatcgcct atgcgaaaaa aggcattcgc
     1801 tgcaacaccg tgatccccgg actgatgcat accgcgctcg tcgagcatcg cttggccaaa
     1861 accatcggtg ccaacgacgt ggaagcgctg gtcgccaaac gcaatgcgca atgcccaacc
     1921 ggcaagatgg gcgatgcctg ggatatcgcg cacgccgtcc tgttccttgc ctccgatgag
     1981 gcgcgctata tcaccgccgc ggaaatcttg gtcgacggcg ggctgacggc gacaacgggc
     2041 gtgggctaac gggcggccgc ggtggcgccc accaatcccg cggatcaacg cttgggtagg
     2101 cgctcgaagt tgggcagatc ctcgggaatg tggtgaaagg cctgcttgtc gatggtgtaa
     2161 atggcgagct tgggcgtgcc aaattggctt ggatcatcga gcgtcccgac cttcacgatc
     2221 acggccggga aaccctgcgg gcgggtggca agatgggtgc cgcactcggc gcagaactcg
     2281 cgcgtgacgg ggcgttccag atcgctgcgc cgaaacgctt tgggctgctc cttggtatag
     2341 cgaaagccgc tcacgggcat ggcgatgaaa aaattgggag cgccgccggt gatatattgg
     2401 cattcgcggc agtggcattg tgcctgcatc atcggttcgc cttcggcgag gtagcgcacc
     2461 ttgccgcaat agcatccgcc ttccagtcgc atggctcatc tccgggctcg gccagggctt
     2521 gcgcctcgtt tagcccttgc taatgctgtg cgcaacgccg gcgccgcttg ggtcccgccc
     2581 gcggcggggc aacgccttgc cagaccgggc gttttcaacc ggttgccggc agcagtctct
     2641 tttccgccca aatgccgctt gataggctct gaagtcgcac gctttcgagc ggcgccaggg
     2701 atgagcccgt gacgcaggat gagccggact gccgcatcta tctcgtggtc gaggccgcgc
     2761 cggcggcgat tgaacacctg agcgcggccg ccgcagtcgc cgatatcgcc tgctgcctca
     2821 ttctccccgc cagcggcgcg cccgacgccg cctcacgcgc ccgtccgatt gtcgccgcgg
     2881 cgcaacaggc cgggattgcc gccttgatcg ccggcgatgc cgacctggcg cgggcgctgc
     2941 gcgccgatgg cgtgcacctc gccccctccc cccaaatcct tgccgcctac accgcggcgc
     3001 gccagatgct gggacgcgag gccatcgtcg gggccgatgc cggcgtgtcg cggcacgatg
     3061 ccatgtcgct ggcggaagcc ggcgccgact acgtcgcctt cggggcaccg tcccacctca
     3121 aggaccgtgc ccgcgcccgc gagcggcgcg acgcgctcct cgcctggtgg tcggaaatct
     3181 tcgaggtggc cggcgtggcc ttggacgtcg aggcggccga tgaagcggag cggttggcgc
     3241 gcgcaggtgc cgacttcgtc gccgttgccg tgcccgccgc gtccgccgcc cccacgggcg
     3301 aactcctcgc caagatcggc gccgccatcg gcgccaatgt ggaggcgggt tgaggaatag
     3361 gcgatgcgct cgatcttcgt ccttgtcctg accttaacgc tgctcgcgcc ttcggcgcac
     3421 gcggaaacga gctcgtggac atcgcagggc gatgcggcgg gaaaaaaagg cacggcgccg
     3481 gcgaaagcgg caaagccggg cgccgccaag tcggcgaccc ccgcgccgag caccagtgca
     3541 gcccctgccg gcgacggcgt cagcagtcac gccaaaggcg ccacccaagg cgaggacccc
     3601 gcctacgagg cttttgacca aggccgttat ctcacggcct tggagctggc cgtgaaggcg
     3661 gccgagcagg gtgacccgca ggcgcacacg ctggttggac gcctctatgg cgagggctac
     3721 ggcgtggcaa agaattccgc gctcgcggcc aaatggtacg cgcgcggcgc cgagctgggc
     3781 gacccggaag ccatgttcgc ctacgccatg atgctggtgg aaggccaggg catcgacaag
     3841 gatcccgtcg cggcagcccg ctacttcgag gccgcggcgg tgcaccgcca cgcgcttgcc
     3901 aactacaatt tggcactcct ctttctgcgc ggcgagggca aacccgagaa cccctatcgc
     3961 gcgttcatgc acatgcagtt tgcggccgaa gccggcgtta cggcggccca atacgatctg
     4021 ggcaccctct atgcgacggg caccggcgtt gaacccaatg ccttcgaggc cgcgcgctgg
     4081 attggcaagg ccgcggccgc cggacacccc gaagcggcgc tcgagttcgg cgtcatcctg
     4141 tttcgcggtc acggcatccc ggtcgatgag aagcggggcg tggagatgtt tcgggccgcc
     4201 gccgagaagg ggctcgcctc ggcgcaagat cggctagcgc gctgctatgt gtatggcgcc
     4261 ggggtggaga aaaatctggt gcagggggcc aaatggtact tgatcgccaa ggccggcggc
     4321 ctcgaggatg acgggcttga aaaagtgcta acccagctct cgagcgccga tcgcagcaaa
     4381 gcccagaagg ccgccagcga gtggcgcgag cgcgcacagg tcggcatgcc atagcctgcc
     4441 gagacagcca accgcgttcg gcctcagtcc gcggccgtga cccgagccat cgccccttcg
     4501 cgctgcttga agttttgata ggtggccaag taggccttca ccgccatgcc caccacgtgg
     4561 ctgatacgcg ccggcgtcgg cggaccggcg gcattgaaca gcatgggttt gaaggtcgtc
     4621 gatacgcagg catcgatgaa ttgcgcggcg gccacttccg tatcgtgcgg ctcgagccca
     4681 ccctgggcga ttttgtcctc caggtagcgc ttcagactgc cgatgccgag ggcgggacct
     4741 gaggcgtaga actgggcgcc gatctcgggc atgcgatcgg caatggcaat gatggtccgc
     4801 aaggacggca ccccgtcggg ccggcacaaa aagcgcgtca gttcctcacc gagccgctgc
     4861 agttcgctgg cgatcggcgc ctcgcggtcg agcgtgaaga tctgctcggc ctcggcccgg
     4921 cactgctcct cgacgatggc ctcaaacagc tcttctttgc tcttgaagta gacgtaaagc
     4981 gtgcccttgg agacccccgc ctcgcgggca atcgcaccca tgctggccgc gtcaaagccc
     5041 tgggcgagga acatgtgccg cgccccttcc aggatctggc gccgcttcag ccgctcgccg
     5101 cgcgcctcgg gcgctagccc tttgtccacg tccgtcgcca tgacacgctc tctttcgccc
     5161 tctcggtgtc ggcccttttt cggcctcccg gcggctcaca agctagtgac cgaaccgttc
     5221 ggtcattgac atatggtgac ccaccgccta tgtcaagggt cgctgaccga accgttcggt
     5281 taggggtcga ggtgaaaaga tgttcgaaga tcaagaactc aaagacaacc ccatggatcg
     5341 gagccccgag ggcggcgagt acgcgcctct ctcccagccc gccgctgccc cgagctcggg
     5401 cgtagcgccg cggcggcggc tcgtcgtttt ggcggtcctc gtcggcctcg cgctggccgc
     5461 cgcggggacg acctatgggc gctattggct gacccagggc cgctatctcg tcagcaccga
     5521 tgacgcctac gtcggcgcca aaagcgcaac gttgtctccc aaggtctccg gctacattgc
     5581 cgaagttgcc gtcgaggaca acgcccaggt cgccgccggc gacgtgatcg cccgcatcga
     5641 tccgggcgac tatcagctgg cggtggcgaa cgcgcgcgat cagatcggcg tgcaacaggc
     5701 aaccatcgac cgcctcggcc ggcagaccac cg
//
LOCUS       DASTMT010000527         2732 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_297890, whole genome shotgun sequence.
ACCESSION   DASTMT010000527 DASTMT010000000
VERSION     DASTMT010000527.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2732
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_297890"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..535
                     /locus_tag="VFR00_12120"
     CDS             <1..535
                     /locus_tag="VFR00_12120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006021568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0160059.1"
                     /translation="WSQDFALLLKAAADAGLQVNWYTYYAGGTGGPTSVKQTGLNHRV
                     FAIQEGSANVDHAASQATEKAFREKFGVSNFYPRAFNQMRMLAAAINKANSTDPTKIA
                     LALEDMKFEVFNGGDGFMRKDDHQFFQPMYIVSLGDLGPKEPFDEEKTGWGWRTIARL
                     DTKDTILPTSCKMNRPS"
     gene            606..1556
                     /locus_tag="VFR00_12125"
     CDS             606..1556
                     /locus_tag="VFR00_12125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011502000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEU0160060.1"
                     /translation="MAELILISSLNGVLYGLLLFLMASGLTLIFSMMGVLNFAHASFY
                     MLGAFLGFQISRWAGFWPALILAPVLVGLAGALVERFGLRNVHRHGHVAELLFTFGLA
                     FVIEELVQIVWGKSPVDFRTPALLDFPAFTVFATNYPAYKLFMLVISIAIFVGLFVML
                     TQTRIGLIVQAALTHPHMVAHLGHNVPFVFMSVFGLGAALAGIAGVIAGPALVTQSSM
                     AALLGPILFVVVVVGGLGSLQGALFASLLIGLLQTFAISINLSLSDIFAGSGFRPEPN
                     TLLGDLWRVTVAQIAPILPYVLLILVLIFKPTGLFGNRET"
     gene            1553..>2732
                     /locus_tag="VFR00_12130"
     CDS             1553..>2732
                     /locus_tag="VFR00_12130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006021570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEU0160061.1"
                     /translation="MSDAILTRAASAAAQTPLGRLAPYGAWIAAALLLALLPLVFRSG
                     TALTMLSLMGIAIIFALSYNMLLGQTGMLSFGHAVYYGLGAYFTVHAINAAIAARLPV
                     PLIVMPVVGGFAGLLFAVIFGWVSTKRSGTAFAMISLGLAELVGSSSLILRSFFGGEE
                     GVTTNRTKLVRLFDWNFGPQLQVYYLIAAWCLLCIALMYALTRTPFGRMCNAVRDNSE
                     RAQFVGYNPQVVRYLAFCLSGLFAGVAGGLTSINFELANSALFSAAQSGNVLLATFIG
                     GAGHFVGPILGAVLVTYLQNMLSDVTEVWQLYFGLLFIATVMYAPGGLAGLIMMHGPI
                     WRAGALAQLLAAYALMLLPIAALALGAILVIEMMTHLGVKAAEGTLMKMGGITFDAKS
                     P"
BASE COUNT          392 a          930 c          835 g          575 t
ORIGIN      
        1 ttggagccag gacttcgccc tgttgctaaa ggcggcggcc gacgccggcc tgcaggtgaa
       61 ctggtacacc tattatgccg gcggcaccgg tgggccgacc tcggtcaagc agacgggcct
      121 caaccatcgc gtctttgcca tccaggaagg ctcggccaac gtcgaccatg ccgcctcgca
      181 ggcgaccgaa aaggccttcc gcgaaaagtt cggcgtgtcg aatttctatc cgcgcgcctt
      241 caaccagatg cgcatgctgg ccgccgccat caacaaggcc aactccaccg atcccaccaa
      301 gatcgcgctg gcgctcgagg acatgaagtt cgaggtcttc aacggcggcg acggcttcat
      361 gcgcaaggac gaccaccagt tcttccagcc catgtacatt gtgtcgctgg gcgaccttgg
      421 ccccaaggag ccgttcgacg aggagaagac cggctggggt tggcgcacca tcgcccggct
      481 cgacaccaag gatacgatcc tgccgaccag ctgcaaaatg aaccgtccga gctgacggtc
      541 cgcgccgaag gggccgccgc acccgcgaga catcggcggc cctcgcgcac ccgggagggg
      601 gcccagtggc ggaactgatc ctgatctcca gcctcaacgg cgtgctctac ggcctattgc
      661 tgtttttgat ggcgagcggc ctcacgctca tcttttccat gatgggcgtg ctcaacttcg
      721 cccacgccag cttctacatg ctgggggcct tcctcggctt tcagatcagc cgctgggccg
      781 gcttctggcc ggccctgatc ctggccccgg tgctggtcgg cctggccggc gccctggtgg
      841 aacgcttcgg cctgcgcaac gtgcacaggc acggccacgt ggccgagctg ctcttcacct
      901 tcggcctcgc cttcgtcatc gaggagctgg tgcagatcgt gtggggcaag tcgccggtcg
      961 atttccgtac cccggcgctg ctcgactttc cggcctttac ggtgtttgcc accaactacc
     1021 ccgcctacaa gctgttcatg ctggtgatct cgatcgccat cttcgtcggt ctgttcgtga
     1081 tgctgacgca gacgcgcatt ggcctgatcg tgcaggccgc gttgacgcat ccgcacatgg
     1141 ttgcccatct cggccacaac gttcccttcg ttttcatgtc ggtgttcggc ctcggtgcgg
     1201 cgctagccgg catcgccggc gtcatcgccg ggccggcgct ggtgacgcag tcgagtatgg
     1261 cggccctgct cgggccaatt ctgttcgtgg tggtggtggt cggcggcctc ggctccctgc
     1321 agggggcctt gttcgcctcc ctgctgatcg gcctgctgca gacctttgcc atttccatca
     1381 atctgtcgct gtccgacatc tttgccggct ccggcttcag gcccgagccg aatacgctgt
     1441 tgggcgattt gtggcgcgtc acggtggccc agatcgcccc gatcctgccc tacgtgctgc
     1501 taattctcgt cttgatcttc aagccgaccg gcttgttcgg gaaccgagaa acatgagcga
     1561 tgccatcctg acgcgcgccg ccagcgcggc ggcgcaaacc ccgctcggtc gcctggcacc
     1621 ttacggcgcc tggatcgccg cggcgctgct gttggcgctg ttgcccctcg tcttccgctc
     1681 cggcacggcg ctcaccatgc tgagcctgat ggggatcgcc atcatctttg ccctctccta
     1741 caacatgctg ctcggccaga ccggcatgct ctccttcggg catgccgttt attatgggct
     1801 tggcgcctac ttcacggtgc acgccatcaa tgcggccatc gccgcccgcc tgcccgtgcc
     1861 cctgatcgtc atgcccgtgg tcggcggctt cgccgggctc ctgtttgccg tgattttcgg
     1921 ctgggtgtcg accaagcgca gcggcaccgc gttcgccatg atctcgctgg gtctggccga
     1981 gctggtcggc tcgagctcgc tgatcctgcg ctcgttcttt ggcggcgaag aaggtgtgac
     2041 caccaaccgc accaaactcg tgcgcctgtt cgactggaat ttcgggccgc agctgcaggt
     2101 ctactatctg atcgccgcct ggtgcctgtt gtgcatcgcc ctgatgtatg cgctgacgcg
     2161 cacgcccttt ggccgcatgt gcaatgccgt gcgcgacaat tccgagcgcg cccagttcgt
     2221 cggctacaat ccgcaggtcg tgcgctatct cgccttttgc ctatcggggc tgtttgccgg
     2281 cgtcgccggc gggcttacct cgatcaattt cgagctcgcc aattcggccc tgttcagcgc
     2341 ggcgcagtcc ggcaacgtcc tgctcgccac cttcatcggc ggtgccggcc acttcgtcgg
     2401 gccgatcctg ggcgccgtgc tcgtcaccta cctgcaaaac atgctgagcg acgtcaccga
     2461 ggtctggcag ctctattttg gcttgctgtt catcgccacc gtcatgtacg cgccgggcgg
     2521 cctggccggg ctcatcatga tgcacggacc gatctggcgc gccggggcgc tggctcaatt
     2581 gctggcggcc tatgcgctga tgctgctgcc gatcgccgcg ctggcgctgg gggcgatcct
     2641 ggtgatcgag atgatgacgc atctgggcgt caaggcggcg gaaggcacgc tcatgaaaat
     2701 gggcggcatc acctttgatg ccaagagccc aa
//
LOCUS       DASTMT010000528         3047 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_4234026, whole genome shotgun sequence.
ACCESSION   DASTMT010000528 DASTMT010000000
VERSION     DASTMT010000528.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3047
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4234026"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            196..645
                     /locus_tag="VFR00_12135"
     CDS             196..645
                     /locus_tag="VFR00_12135"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016279 - protein-lysine
                     N-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein"
                     /protein_id="HEU0160062.1"
                     /translation="MLPRLQKVTGRENGLPRLSSKLKARYPLQVKASRIAGRGVFAVK
                     DIPWGRKIIRYQGAVIDDTEAAKRVAQGATAIMELGAGRNLDGFDGGNGAAWINHSRR
                     RPNCFLLREDDEIWIVAGIEGISAGDELTYDYGSEYYPRRNGRSTAG"
     gene            694..1062
                     /locus_tag="VFR00_12140"
     CDS             694..1062
                     /locus_tag="VFR00_12140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0160063.1"
                     /translation="MDVLAWLWWVISTVLGVVLALIWFLVSGWVSTLLQIAFLVIVVY
                     FLKYGWQRAPAEIWRRTRSFAQFFLGWIRAREPAPVAQEASVRVVKSKEFGDINISTL
                     LSLMMLAGLLLASRLEGLAQ"
     gene            1059..1400
                     /locus_tag="VFR00_12145"
     CDS             1059..1400
                     /locus_tag="VFR00_12145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0160064.1"
                     /translation="MISWLKWSAARAPILPVAWVLAHATVLLWKALDPRYIDEASWIV
                     DTILKPLHPLGVSLVSFVMPPLRTLVSNMPPTHLDADKVLYGFLVPIYMGIIIGFFRM
                     LRWLVAPAPRF"
     gene            1700..2437
                     /locus_tag="VFR00_12150"
     CDS             1700..2437
                     /locus_tag="VFR00_12150"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU0160065.1"
                     /translation="MRSRCSDRTADFFVCRGWRSPRTLLGSVSTACGVRRSPRPWAAS
                     LVALALVTGVAHAGAALGQPVPAWVRCAGGNSISSAGQIGGCTILIHSGRENAQGLAK
                     AFSHRGTAYFAKGDYRRAIQDYSQVINLDPSNATAFDNRCWTRATLGLLGEALQDCDQ
                     SLKLRPNHAGTLSTRGFAYLKSGNLDAAIADYNAALATAPQNAYALYGRGLARLKKAD
                     PDANEDIKAANAISNDIALEFARYGLK"
     gene            2829..>3047
                     /locus_tag="VFR00_12155"
     CDS             2829..>3047
                     /locus_tag="VFR00_12155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0160066.1"
                     /translation="MSHDVLLKEKAMEALLSHPIKHGRLTITAPDGQRHTLKGAMNGP
                     EVSIKVTDSKVMRRMLVIPDLYLGEAYMD"
BASE COUNT          549 a          973 c          991 g          534 t
ORIGIN      
        1 tcgcgcgcgg caagttcgtg caaatgaaag aggaagcgac caaaaaggtc gaggagctga
       61 agaaggacct tggcgacaag tgggacgaga tcacgggaaa gaagagttga gggctcccag
      121 ccgcccagcc gcagctctgg cgcccgcgcc cggggcagac ggccgcacag caggcgaggc
      181 ggcgccggcg ctgcggtgct cccgcgcctg cagaaagtga ccggccgcga gaatggcttg
      241 ccgcgcctaa gcagcaagct caaggccagg tatccgctgc aagtaaaggc gtctcgcatc
      301 gccgggcgtg gagtcttcgc cgtcaaggat attccctggg gtcggaagat cattcgctac
      361 caaggcgcgg tgatcgacga cacggaggca gcaaagcgcg tggcgcaggg cgcgacagcc
      421 attatggaac tcggcgccgg gcgcaacctc gatggatttg acggcggcaa tggcgccgca
      481 tggatcaatc acagtcgacg ccggccgaac tgttttctgc tgcgggagga cgacgaaatt
      541 tggatcgtcg cgggcattga aggtatctcg gccggcgacg aactcactta tgattacgga
      601 agcgaatatt acccacgcag gaatgggcgc tcgacggctg ggtgacctca ctagcctgcg
      661 cgggtgccgg cttcgttgca tagagggccc cccatggatg ttcttgcgtg gctttggtgg
      721 gtgatatcga cggttcttgg tgtggtcctc gccctgattt ggttcttggt gagcggttgg
      781 gtttccacgc tcctgcaaat cgccttcttg gtgatcgtcg tctacttcct gaaatatggc
      841 tggcagcggg ccccggcgga gatttggcgc aggacacgat ccttcgctca gttctttctg
      901 ggttggatcc gcgcgcgcga gccggcgcca gtagcccagg aggcgtccgt gcgcgtcgtc
      961 aagagcaagg aattcggtga tatcaatatt tcgaccctcc tctcgcttat gatgttggca
     1021 gggctccttc ttgcttcacg tctcgagggt cttgcacaat gatctcctgg ctcaagtggt
     1081 cagcggcgcg cgcgccgatc ctgccggtcg cctgggtcct cgcccatgcc acggtgctgc
     1141 tgtggaaggc gctcgatccc cgctatatcg acgaagcaag ctggatcgtc gacaccatac
     1201 tgaaaccgct gcatccgctc ggcgtgtcgc tggtgtcgtt cgtcatgccg ccactgcgca
     1261 ccctggtcag caacatgccc ccgacccatc tcgatgccga taaagtgctg tatggcttcc
     1321 tggtgcccat ctatatgggg atcatcatcg gcttttttcg catgctgcgc tggctggtgg
     1381 cgcccgcgcc gcgcttctag gggttgtcgg cgcgttggga cctcggggcg cgggctttgc
     1441 cgggcccgcg cgcgcccagc ccgcgggcgg catgccttag cgcggtcgcg gaccatcggt
     1501 ttggtgcggg ggagacgagg ggacgacggc gccatcgtta ggacaaacga gcctgcgcaa
     1561 atccgccggg caccgtgcag gctaggccgc ccaccgcggc gccgcggccg gcccggtgca
     1621 aggccttcaa gccattgcga acgaccatcg atccagcggg gaagcgccga cacggccgag
     1681 ccggggcgct tgtgagggga tgcgcagtcg ctgctccgat cgtacagccg actttttcgt
     1741 ctgccgcgga tggcgcagcc ctcgaacgct gcttggcagt gtctcgaccg cctgcggcgt
     1801 ccgacgctcg ccgcggccgt gggcggcgag cctggtcgcg ctggcgctcg tcacgggtgt
     1861 ggcccacgcc ggcgcggcgc tggggcaacc ggtgccggcg tgggttcgct gcgcgggcgg
     1921 caacagcata tcctctgctg gccagatcgg cggctgcacg attttaatcc attccgggcg
     1981 cgagaacgcg caaggtctcg ccaaagcctt ttcgcaccgg ggaaccgcgt atttcgccaa
     2041 aggcgattac cggcgcgcca ttcaagacta cagccaggtc atcaacctcg accccagcaa
     2101 cgccacggcc tttgacaacc gctgctggac ccgcgcgacg ctcgggctcc tcggggaagc
     2161 tttgcaggac tgcgatcaat cgctgaaatt acgccccaac catgccggga cactgtcaac
     2221 gcgcggcttc gcctatttaa agtccggcaa tctcgatgcc gccatcgccg actacaacgc
     2281 cgccttggcc accgcgccgc agaatgccta tgccctctat ggacgtgggt tggcgcggtt
     2341 gaaaaaagcc gatcccgacg ccaacgagga tatcaaggcc gccaacgcca tcagcaacga
     2401 tatcgcgctc gagtttgcgc gctacggcct gaagtaaatt cgcggggtag cgtgccggat
     2461 gaggggatgc cacccccgcc gacattgccc cgatccgccc gcgcgcggcc ggccccggca
     2521 agcgcggcgg gcgccgcgag gccaaaggcg gagcgccgcc cgcgccaccc ccaggcgggc
     2581 ggtgagccgc tgccgccgct ttttccacag cctggcgcgt ccgctgaagc aaaacgcctc
     2641 ctcagggcgg tgcgcgccct gccataatct ccgccacaaa gcccagtgca atggcgtcgt
     2701 agccaatagc gctcgtgcaa ggcgaaaacg ggcgcacgcg ttcatcgctc acgtcaacgg
     2761 ttcgaggcaa acccccggcg cgcgcacctc acctccggcg gattgcggac gagccgaaga
     2821 aagaagccgt gagccatgac gttctgctca aggagaaagc catggaggcc ttgctaagtc
     2881 acccaatcaa gcacgggcga ttgaccatca cggcgccgga tggtcagcgt cacaccctta
     2941 agggcgcgat gaatggacca gaggtgagca tcaaagtcac cgacagcaag gtgatgcgcc
     3001 gcatgctggt cattcccgac ctttatctgg gtgaagccta tatggat
//
LOCUS       DASTMT010000529         4412 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_1940932, whole genome shotgun sequence.
ACCESSION   DASTMT010000529 DASTMT010000000
VERSION     DASTMT010000529.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4412)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4412)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4412
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1940932"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            317..616
                     /locus_tag="VFR00_12160"
     CDS             317..616
                     /locus_tag="VFR00_12160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0160067.1"
                     /translation="MARGIAEAVRWAVLAAALAALAGCAEEGGSYGGDPGARPLAAGQ
                     SCGSIRQELNRLDSRGTQAKVEAATQGKKLPPAAQADVDRYNDLLNQYLGARCHV"
     gene            726..2147
                     /gene="lpdA"
                     /locus_tag="VFR00_12165"
     CDS             726..2147
                     /gene="lpdA"
                     /locus_tag="VFR00_12165"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008968640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEU0160068.1"
                     /translation="MAENSFDIIVIGSGPGGYVTAIRAAQLGFKTAIVEREYLGGICL
                     NWGCIPTKALLRSAEIYHYLTHAQNYGLSAGAVGYDAKAVVERSRKVAKRLNDGVAFL
                     MKKNKIAVIWGEAALEAPGRVAVKSGKAAAPKGTLGPGQYQAKHIIIATGARPRVLPG
                     LEPDQRLVWTYFEAMLPAAMPKSLLVVGSGAIGMEFASFYRSFGAEVTVVEILPQILP
                     VEDAEIAQFARKAFERQGMKILTNTKVTQLAKQADSVTASIDDGKGGTQQITAERVIS
                     AVGVVGNIENLGLERLGVKTSRGTIDTDALSRTSVPGIYAIGDVAGPPMLAHKAEHEG
                     VVCVEAIKGLQPHPLDKAKIPGCTYCQPQIASVGLTEKAAKDQGRAVKIGRFPFVGNG
                     KAIALGEDQGLVKTIFDANSGQLLGAHLVGAEVTELIQGFVIAMQLETTEEDLMHTVF
                     PHPTLSETMHESVLDAFGRVIHV"
     gene            2435..2698
                     /locus_tag="VFR00_12170"
     CDS             2435..2698
                     /locus_tag="VFR00_12170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018899984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEU0160069.1"
                     /translation="MNMNAQQLIIMAVIGIVAGWLASVLVGGPGSLLGYLITGIIGAF
                     VGSFIFSSAGWNLNLGNAWVDSIVTALIGSVVVILLARLILGW"
     gene            complement(2664..4010)
                     /gene="purB"
                     /locus_tag="VFR00_12175"
     CDS             complement(2664..4010)
                     /gene="purB"
                     /locus_tag="VFR00_12175"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009494126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEU0160070.1"
                     /translation="MIPRYTRPEMAEVWSAQTRFRIWFEIEAHAASAMAELGMIPKEA
                     AAAIWEKGSKATFDVERIDAIEREVKHDVIAFLTHVAELVGPEARFLHAGLTSSDILD
                     TAFNLQLVRAADLLLVDLEQLLAALRRRALEHKHTVTVGRSHGIHAEPTTFGVKLAYA
                     WAEFARARERLLCARGEVATCAISGAVGTFAHVDPRVEAYVAKKLGLTPEPVSTQIIP
                     RDRHAMYFATLAVIASSLERLAVEIRHLQRTEVLEAEEYFAPAQKGSSAMPHKRNPVL
                     SENITGLARMVRSYALPAMENVALWHERDISHSSVERMIGPDATVTLDFALHRMTGVI
                     DRLLVYPKNMRRNLDRLGGLVHSQRVLIALTQKGMTREQAYRLVQKHAMRVWQGEGDF
                     LGLLTADAEVSKVLGARELEALFDLEFHTKHVDEIFARVFGAAAAATSPVSGAPAR"
     gene            complement(4090..>4412)
                     /locus_tag="VFR00_12180"
     CDS             complement(4090..>4412)
                     /locus_tag="VFR00_12180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006206088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HEU0160071.1"
                     /translation="CADESCHTASDLDSLVGKYDAINIKLDKAGGLTEGLALAAAARA
                     RGFKVMVGSMVATSLSMAPATLLAQDADWVDLDGPLLLARDREPSLEYEGARLKPPSP
                     ELWG"
BASE COUNT          781 a         1455 c         1456 g          720 t
ORIGIN      
        1 cagcgccgcg gcaggctcaa ggcacgctcg cggggcgcca ttaggcgccc cgcgcgcccc
       61 tcgggggcag ggggcttcgc caccaaagtt gtgtccgggc cgcgcgaccg ccagggcctc
      121 agcgccacaa ggggggtggg cgggaggagc cggggcgtgc gtcaagcctc gaatggcgtt
      181 ggaaaaaaaa cggcactgcc atcggcttgc cgtgctgcaa agttaccaca ggcagctgct
      241 agcgggttga acttgctgcg cccttatggt tcgtagcggc gagcggagcg acgcgatggg
      301 aagcaaggag gccggcgtgg cccgaggcat agctgaggcg gtcaggtggg cggtgttggc
      361 agccgcgctg gcggcgctgg caggctgcgc cgaagagggc ggcagctacg gcggggatcc
      421 gggagcgcgg ccgttggccg ccggccagag ctgcggctcg atccgccagg agctcaaccg
      481 gctcgatagc cgaggcacac aggccaaagt ggaagccgcc acccagggca agaagctgcc
      541 gccggccgcg caggccgacg tcgaccgcta caacgatctc ttgaaccagt acctcggcgc
      601 acgctgccac gtttgagcgc ccgggcctgg cccgaactgc ttgcgatttg ccttgaaacc
      661 gcaggctcca gccactaggc cggggcctgt ggtcgcttgc gcgccttcct gttgagaaca
      721 cccccatggc agagaacagc ttcgacatca ttgtgatcgg ctccggccct ggcggttacg
      781 tgaccgccat ccgcgccgcc caactcggct tcaagaccgc catcgtcgag cgcgaatatc
      841 tgggcggcat ctgtctcaac tggggctgca ttccaaccaa ggccctgttg cgctcggcgg
      901 agatctacca ctacctcacg cacgcccaga actatggcct gagcgccggc gcggtcggct
      961 acgatgccaa agcggtggtc gagcgctcgc gcaaggtggc caagcgcctc aacgacggcg
     1021 tcgccttcct catgaaaaaa aacaagattg ccgtgatctg gggcgaggcg gcgctggaag
     1081 cgccgggtcg cgtcgccgtc aagtcaggca aggccgcagc gcccaaaggc acgctcgggc
     1141 cgggacagta ccaagccaag cacatcatca tcgccaccgg cgcgcgcccg cgcgtgctgc
     1201 cggggcttga gcccgatcag cgcctcgtgt ggacctattt cgaggccatg ctgccagccg
     1261 ccatgccgaa gtcattgctc gttgtcgggt cgggcgcgat cggcatggag ttcgcctctt
     1321 tctaccgctc cttcggcgcc gaggtcaccg tcgtcgaaat cctaccgcaa atcctgcccg
     1381 tcgaagatgc cgagatcgca caatttgcgc gcaaggcgtt cgagcgccag ggcatgaaaa
     1441 ttctcaccaa caccaaggtg acgcagcttg ccaagcaagc cgatagcgtc accgccagca
     1501 tcgacgacgg caagggtggc acgcagcaga tcacggccga gcgcgtcatc agcgccgtgg
     1561 gggtggtcgg caacatcgag aacctcggcc tcgagcgcct gggcgtgaag acgagccgcg
     1621 gtaccatcga caccgatgcg ctcagccgca ccagcgtgcc cggcatttat gccatcggcg
     1681 acgtggcggg gccgcccatg ctggcgcaca aggccgagca cgaaggcgtc gtgtgcgtgg
     1741 aggccatcaa aggcttgcag ccgcacccgc tcgacaaggc caaaatcccc ggctgcacct
     1801 attgtcagcc acagatcgcc agcgtcggcc tgacggaaaa ggccgccaag gaccagggcc
     1861 gtgccgtcaa gatcggccgc tttcccttcg tgggcaacgg caaggccatc gcgctgggcg
     1921 aggatcaagg cctggtcaag acgattttcg acgccaacag cggccagctg ctcggcgcgc
     1981 acttggtcgg cgccgaggtg accgaattga tccaaggctt cgtcattgct atgcaactcg
     2041 aaaccaccga ggaagacctg atgcataccg tcttcccgca cccgacgctt tcggaaacca
     2101 tgcacgaaag cgtgctcgat gccttcgggc gcgtcattca cgtctaaggc gcgcgccaag
     2161 caaccgcgcc tgcgcgcagg tgtcggaccg ccccggcgcc agccggtccg cctcgcgctg
     2221 accagcgctg agcgcacgcg ccggtgcggt ggcgacgccc gggcccagac cctttcagcc
     2281 cttcgcgccc tgccgcaagc gccgccatct cgcggatgcg aagcataaag gtcgacggcc
     2341 gcctgcgagt cggtcagact ccgtgcccag aggattgcct gcctcacggg gcaaaaggag
     2401 gcaggccgcc cgctgccaga ggggagactt gcacatgaac atgaatgcac agcagctcat
     2461 tatcatggcc gtgatcggca tcgttgccgg ctggctcgcc agcgtgcttg tcggtggtcc
     2521 gggaagcctg ctcggttatc tcatcaccgg tatcatcggc gccttcgtcg gctccttcat
     2581 cttcagctcg gcgggctgga acctgaatct cggcaacgcc tgggtggatt cgatcgtcac
     2641 ggcgctgatc ggttcggtcg tcgtcatctt gctggcgcgc ctgatactgg gctggtagcg
     2701 gccgccgccg cgccgaacac gcgggcgaaa atctcatcga cgtgcttggt gtggaactcg
     2761 aggtcgaaca aggcctcgag ttcgcgcgcg ccgaggactt tcgagacttc ggcatctgcc
     2821 gtgagcaggc cgagaaagtc cccttcgcct tgccacaccc gcatggcgtg cttttgcacc
     2881 aggcgatagg cctgctcgcg ggtcatgcct ttttgcgtca gcgcgatcag cacgcgttgc
     2941 gagtgcacca ggccgccgag ccggtctagg ttgcggcgca tgttcttggg atagaccagc
     3001 aggcgatcga tcacgcccgt catgcgatgg agcgcgaagt cgagggtcac cgtcgcatcc
     3061 ggcccgatca tgcgctccac cgaggagtgc gagatgtcgc gttcgtgcca gagcgccacg
     3121 ttttccatgg ccggcaaggc gtagctgcgc accatgcgag cgagcccggt gatattctcg
     3181 ctgagcacgg ggttgcgctt gtgcggcatg gcggaggagc ctttctgcgc cggcgcgaag
     3241 tactcctcgg cttccagcac ctcggtgcgc tgcagatgcc ggatctcgac ggccagccgc
     3301 tccagcgagg aggcaatcac ggccagcgtg gcgaaataca tggcatggcg atcgcgcggg
     3361 atgatttgcg tcgataccgg ctccggcgtc aggcccagct tctttgccac ataggcttcc
     3421 acgcgcggat cgacatgggc gaaggtgccg acggccccgg aaatggcaca ggtggcaacc
     3481 tccccgcggg cgcaaaggag gcgctccctg gcgcgggcaa attcggccca ggcataggcc
     3541 agcttgacgc cgaaggtcgt cggttccgcg tggatgccgt ggctgcggcc gacggtgacg
     3601 gtgtgcttgt gctccaaggc gcgccggcgc aaggccgcca gcagctgctc gaggtcaacc
     3661 agcagcaggt cggccgcgcg caccagctgc agattgaagg cggtgtccaa gatgtccgag
     3721 gaggtcaggc ccgcgtgcag gaagcgcgcc tccggcccga ccagttcggc cacatgggtg
     3781 aggaaagcaa tcacgtcgtg cttgacctcg cgctcgatgg catcgatgcg ctcaacgtcg
     3841 aaggttgctt tgctgccctt ctcccagatg gccgcggcgg cctccttggg gatcataccc
     3901 agctctgcca tggccgatgc cgcgtgcgct tcgatctcaa accagatgcg aaagcgcgtt
     3961 tgcgcgctcc aaacctcggc catctcgggg cgcgtatagc ggggaatcat gccgtcctcg
     4021 cttggggttg tgcgcttgtc gcctagcacg cgccgccgtt ggcggcaaac gctgacggca
     4081 tgggcaaatc tagccccaaa gctccgggct gggcggcttg aggcgtgcac cttcgtactc
     4141 aaggctgggc tcgcggtcgc gcgccagcag caaaggcccg tccaggtcga cccaatcggc
     4201 gtcctgcgcc agcagcgtgg cgggcgccat cgacagcgag gttgccacca tcgaacccac
     4261 catcaccttg aagccgcggg cacgggcggc ggcggcaagc gccaggcctt cggtcagccc
     4321 gcccgccttg tcgagtttga tattgatagc atcatatttt ccgaccaaac tatcgagatc
     4381 gcttgccgta tgacacgatt cgtcggcaca ga
//
LOCUS       DASTMT010000530         3539 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_2923107, whole genome shotgun sequence.
ACCESSION   DASTMT010000530 DASTMT010000000
VERSION     DASTMT010000530.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3539
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2923107"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..589)
                     /locus_tag="VFR00_12185"
     CDS             complement(<1..589)
                     /locus_tag="VFR00_12185"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEU0160072.1"
                     /translation="MLKLGVTLYFVRHGETDWNRAQRYQGHRDIPINATGQAQAKRNG
                     GRLGALLGTRAAALDYVASPLKRASETMRIVRAEMGLNAADFRTDDRLKEINYGTWEG
                     QAWHDIASRDRDGYAARQADLWNWQPRGGESYRMLAERVGAWLAEVAGDTVVVAHGGV
                     SRALRALILGLSAEDVMRLEVPQDLILVIEGGSVRW"
     gene            701..1492
                     /locus_tag="VFR00_12190"
     CDS             701..1492
                     /locus_tag="VFR00_12190"
                     /inference="COORDINATES: protein motif:HMM:NF018420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1194 domain-containing protein"
                     /protein_id="HEU0160073.1"
                     /translation="MGADLLAKRACLYAHIALLAALGLALAPGGVPQAGAQGNVDLQL
                     VLAVDVSGSVSEARFELQKRGYVAAFRDPRLLRAIQSGSQQRIAVTMVQWTGPVLQVQ
                     VVPWQFISGEASMQALADAIEATPRQLFGGGTSISGAIDYAMSLFAGAQGQGGRRVID
                     VSGDGTNNRGRPVTEARDDALRAGVSINGLPILALDPELDQYYLHNVIGGPNAFVIAA
                     ASYETFADAILKKLVTEIATAPPRQARTRLAKLMLRRLRAQAALL"
     gene            1697..2578
                     /gene="ugpA"
                     /locus_tag="VFR00_12195"
     CDS             1697..2578
                     /gene="ugpA"
                     /locus_tag="VFR00_12195"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014438746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HEU0160074.1"
                     /translation="MEKRAIFAGKLLPYLLLAPQLLVTLVFFYWPASQALWQSFLLQD
                     AFGLRVDFVWFDNYRDLLAQGDYYRAMATTIVFSLAVTLLSLSIALLLAVQADKSIKG
                     AGAYKTLLIWPYAVAPAIAGVLWLFMFQPSLGLIARGLRGLGLDWNPLLNGTHAMVLV
                     IMAATWKQISYNFLFFLAGLQSIPKSVIEAAAIDGARPVRRFWTIIFPLLSPTAFFLL
                     VVNIVYVFFDTFGIIDTMTGGGPAKATETLVYKIVSDGRLGGDLGSSATQSVILMLIV
                     IALTGMQFRFIERKVTY"
     gene            2578..3327
                     /locus_tag="VFR00_12200"
     CDS             2578..3327
                     /locus_tag="VFR00_12200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015933995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HEU0160075.1"
                     /translation="MRVIWSELTAAELRDKARADAIVLLPVASMEQHGPHLPVGVDSI
                     LCEGICRRAAEAVAAEIPVLVAPTLWCGMAEHHMAFGGTFSFDLSTYRAVLIALTSSI
                     DRHGFKRLIIVNGHGGNIAPLAAILPDLAGQSSLRIRTTTYFELAQPAMPAILEDQDG
                     VRHACEAETSMMMAMAPATVRGDALGHAHGPAHVHRRPLALGQYRSFRDFSPSGVVGD
                     ARRASPEKGEKLIAACTAAIVAVLKDPQTWD"
     gene            3334..>3539
                     /locus_tag="VFR00_12205"
     CDS             3334..>3539
                     /locus_tag="VFR00_12205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012331544.1"
                     /note="with UgpABC is involved in uptake of
                     glycerol-3-phosphate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HEU0160076.1"
                     /translation="MVERRRFGNAFAYLVLSLGVFVIAFPVYLCFIGSTHEQSTIANG
                     QMPITPGALFFQTYYKTLFIGTSGT"
BASE COUNT          549 a         1169 c         1120 g          701 t
ORIGIN      
        1 gccaacgcac gctgccgccc tcgatgacca aaatcaggtc ttgcggcacc tcgaggcgca
       61 tgacgtcctc ggcggatagc cccaagatga gggcgcgcaa ggcccgactg acgccgccgt
      121 gggcaaccac cacggtatcc cccgcgacct cggcaagcca cgccccaacg cgctcggcca
      181 gcatccggta gctctccccg ccgcgcggct gccaattcca cagatcggcc tggcgcgccg
      241 cgtagccatc ccgatcgcgt gaggcgatgt cgtgccaggc ctggccttcc caagtgccgt
      301 agttgatttc cttcagccgg tcgtcggtgc ggaagtccgc cgcgttgagg cccatctcgg
      361 cccgcacgat ccgcatggtt tcgctggccc gcttgagcgg acttgcaaca tagtcgaggg
      421 cggccgcgcg ggtccccaac agcgccccta gccgtccgcc attgcgcttg gcctgcgctt
      481 ggccggtggc attgatcggg atgtcgcggt gaccttgata gcgctgggct cggttccaat
      541 cggtctctcc atgtcgaacg aagtagagcg tgacgccaag cttcagcatt tttgtcgacc
      601 cccctatgct ctcaactggt cgttgtcatc gatgtgcgac aggccgggcc tcgcttgcaa
      661 gcggctcggg cagcgcacct cgccacctcg gatgggtagc atgggtgctg atttgctggc
      721 gaagcgtgcc tgcctctatg cccacatcgc cctgttggcc gcgctcggct tggcgttggc
      781 gccgggcggc gtgccccagg ccggcgccca gggcaacgtc gatcttcagc tcgtcctggc
      841 ggtcgacgtc tccggcagcg tgagcgaggc ccgcttcgaa ctgcaaaaac gcggctatgt
      901 ggcggccttt cgcgatccgc ggctgttgcg ggcgatccaa tcgggctcgc aacagcgcat
      961 tgccgtgacc atggtgcaat ggaccgggcc ggtgctgcag gtccaggtgg tgccgtggca
     1021 gttcatcagc ggcgaggcct ccatgcaggc gcttgcggac gcaatcgaag cgacgccgcg
     1081 ccagttgttc ggtgggggca cgtccattag cggcgccatc gactatgcca tgtcgttgtt
     1141 cgccggggcg caaggccagg gtggccggcg cgtcatcgac gtttccgggg acggcaccaa
     1201 caatcgcggc cggccggtca ccgaggcacg tgatgatgcg ctgagggccg gggtcagcat
     1261 caacggcctg cccatcctgg cgctcgatcc cgagctcgat cagtactact tgcacaatgt
     1321 cattggcggc cccaatgcct ttgtgattgc ggcggcttcc tacgaaacct ttgccgatgc
     1381 gatcctgaaa aaactggtga ccgagatcgc caccgcgccg ccgcggcagg cgcgcacgcg
     1441 cttggccaag ctgatgctgc ggcggctgcg cgcccaggca gcgctcctgt gatctgggct
     1501 cgccgttggg cgagacgccg tcccgtgctg cttttgccgt atgcggggtt ttccgcgcca
     1561 gggtgaggac acggctttgg cgccgcttca ctcgggaaag gcgatgacag gccggcgcgc
     1621 ggcaggctat cacaggagag gctgcgcatc cccgcgggga ggcggagggg cgagagggac
     1681 cgagggggct gccggcatgg aaaagcgcgc cattttcgcg ggaaagctgc tgccctattt
     1741 gctgctggcg ccgcagctcc tcgtcacgct ggtgttcttt tattggccgg cgagccaggc
     1801 gctttggcag tccttcctat tgcaggacgc ctttggattg cgggtcgact tcgtctggtt
     1861 cgacaactat cgcgacctcc tcgcccaggg cgactattac cgggcgatgg ccaccacgat
     1921 cgtcttctcc ctcgccgtga ccctgctatc gctgtccatc gccctgttgc ttgccgtgca
     1981 ggccgacaag agcatcaaag gtgcgggcgc ctataagacg ctgctgattt ggccctacgc
     2041 cgtcgccccg gcgattgctg gcgtgctgtg gcttttcatg tttcagccgt ccctggggct
     2101 gatcgcgcgg gggttgcgcg ggcttggcct cgattggaat ccgcttctca acggcacgca
     2161 cgccatggtg ctcgtgatca tggccgccac ctggaagcag atcagctata attttctctt
     2221 cttcctggcc ggtctgcaat cgattcccaa atcggtgatc gaagcggcgg cgatcgacgg
     2281 cgcccgcccg gttcggcgct tctggaccat tatcttcccg ctgctgtccc ccaccgcctt
     2341 ctttctgctg gtcgtcaaca tcgtctacgt tttcttcgac acttttggca tcatcgatac
     2401 catgaccggc ggcggtccgg ccaaggccac ggaaaccttg gtctacaaga ttgtttccga
     2461 cggtcgcctc ggcggcgacc tgggcagctc ggcgacccaa tcggtgatcc tgatgctgat
     2521 cgtcatcgcc ttgacgggca tgcagttccg cttcatcgag cgcaaggtga cgtactgatg
     2581 cgggtgatct ggagcgagct gaccgccgcc gagttgcggg acaaggcccg cgccgacgcc
     2641 atcgtattgc ttccggtggc ctccatggaa cagcacggtc cgcatctgcc agtcggcgtc
     2701 gatagcatcc tgtgtgaagg gatctgccgc cgcgccgccg aggcagtggc ggcggaaatc
     2761 cccgttctcg tcgccccaac gctgtggtgc ggcatggccg aacaccacat ggcgttcggc
     2821 ggcaccttca gcttcgacct ttccacctat cgcgcggtgc ttatcgcgct caccagcagc
     2881 atcgaccggc atggtttcaa acggttgatc atcgtcaacg gtcatggcgg caatatcgcc
     2941 ccgctggcgg cgattctgcc cgaccttgca ggccagtcct cgcttcgcat ccgcaccacc
     3001 acctacttcg aacttgcgca accggcgatg ccggcgatcc tggaggatca ggacggggtg
     3061 aggcacgcct gcgaagccga gacgtccatg atgatggcca tggcacctgc cacggtgcgc
     3121 ggcgatgcgc ttggccatgc gcatgggcca gcccacgtgc atcgccgacc gttggcgctc
     3181 ggccaatatc gctcgttccg ggacttcagc ccgtcgggcg tggtgggaga tgcgcggcgc
     3241 gcctcgcccg aaaagggcga aaagctgatc gcggcgtgca cggcggcgat cgtcgccgtg
     3301 ctcaaggatc cgcaaacctg ggactgatct tcgatggtcg aacgtcgccg attcgggaac
     3361 gcgtttgcct atttggtgct gtcgctaggc gtgttcgtca tcgcttttcc cgtctacctg
     3421 tgtttcatcg gctcgaccca cgagcaaagc accattgcca acgggcaaat gccgatcacg
     3481 ccgggagccc tattctttca gacctactac aagacgcttt tcatcggcac cagcggcac
//
LOCUS       DASTMT010000531         4253 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_3580547, whole genome shotgun sequence.
ACCESSION   DASTMT010000531 DASTMT010000000
VERSION     DASTMT010000531.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4253)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4253)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4253
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3580547"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(64..2262)
                     /locus_tag="VFR00_12210"
     CDS             complement(64..2262)
                     /locus_tag="VFR00_12210"
                     /inference="COORDINATES: protein motif:HMM:TIGR02123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter permease"
                     /protein_id="HEU0160077.1"
                     /translation="MSTTSTNALSQLPADLDKLEAEHDPEMQFRPLLPRAAWLVAALL
                     FTLSCFHYYTAGFGLLPEVIHRGIHVAFVLGLIFLVFPARRNNSASLPRSSRLRPLGI
                     GLADWLLAIAAALASLYVPWVFHDLQFRVGNPDPIDWIMGTLMLIVVLEATRRSVGPA
                     LSIIAVVLIIYALLGPSLPGIFAHPGNSWKSVVNHLYLTVNGVYGIALGVVATYVFHY
                     VLFGVLATRVGLGRFFIDIATAIAGRFAGGPAKVSIFASGLFGMISGSSIANTVTVGS
                     LTIPMMIRIGYPRHFAGAVESTAATGGQITPPIMGAAAFLMVEYLSIPYQTIIIAAIV
                     PAFMHFFGVFCQVHFEAKKRGMRGMSAAEMPRIGEILRRDWPTSIPLLVLLIVLFSGF
                     TPYLAAFWGITASIVIGVSNRSPIVALGMLGAIAAAELAGELTEWQGVAVILGACGAV
                     SAFYVAKDAGGQQRLLEVLDAFVTGAKYAISVGAAAAAVGIIVGVVTLTGIGFKLSAI
                     ITSTAADLAAVTGSMLPVALFEPKGLTLFYTLVLTAIVCILLGCGVPTTPTYIIMVTV
                     AAPALALMGVAPLIAHFFVFYYGVLADITPPVALAAYAGASMAGADPFKTGNTAFRLG
                     LAKALVPFVFVYSPALLLVSPGFTWTEFCETALGCVVGIVMLAAALSSYGLAPIRGWE
                     RLLLLLAAVLVISPSRVATLAGLGLAVPVLLRQLQAARGGSSPAASPRSS"
     gene            complement(2356..3324)
                     /locus_tag="VFR00_12215"
     CDS             complement(2356..3324)
                     /locus_tag="VFR00_12215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013530643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HEU0160078.1"
                     /translation="MTTTTRRQVLRLGAGLAGAALLHGPAVAQQRFFRIGTGGTGGTY
                     YPIGGLIGNAISSENLNVSAVATNGSVANVNGIIAGAMESGFSQADVNFWAYTGTGIY
                     EGKPKVEELRAIANLYPESVHIVVKKGLGAKSVADLRGKRVSLDEPGSGTLVNAKAIL
                     AAYGLSEKDIKPEYLKQQQCAEKLKDGSLDAYFQTTGYPQGTLSELAATNGFELLPLA
                     GPEAEQLQARFKFFAADEIPAAVYKDVPAVKTLSVGAQWVTSAKLEEGLVYAVTKAMW
                     SDKTRAALDAGHAKGKVIRKETALNGLGIPLHAGAEKFYREAGMLK"
     gene            complement(3474..>4253)
                     /locus_tag="VFR00_12220"
     CDS             complement(3474..>4253)
                     /locus_tag="VFR00_12220"
                     /inference="COORDINATES: protein motif:HMM:NF024986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HEU0160079.1"
                     /translation="NGAALTNANLSGARLQRANLAGAVLRGANAVGAWLQGADLSGAQ
                     LHLANLSNARLQGADLSMAGLEGARLRQAELDGANLTRSILFAADLATARLVGADLTA
                     ALVWRTVPPTNDAVALADFAEIVLRPPRDQDLAQLSVPSRTDDASLEVRLAAESRAWS
                     ASPEHQAWQVLLKPREASDADQYKSRLTGYLVRLMCQSRWADGAVAAGIAKRALGDGF
                     KGEVAAIHARLKSPDCPASAKVAPRLLQELAVAVEASRAQD"
BASE COUNT          851 a         1340 c         1384 g          678 t
ORIGIN      
        1 tgacggcgga aaaaatcgcc gatgccatcg tggcacgcgg cccctaacga cccccggctt
       61 cgtttagctc gagcgagggc tggccgccgg actggagcct cctcttgcag cctgcaactg
      121 gcgcagcaga acgggcacgg caagccccag gcccgcaagc gtcgccaccc gactgggcga
      181 gatcacgagc acggcggcca acagcaagag cagacgctcc caaccgcgaa tcggtgccag
      241 gccgtagctg ctcaaggcag cggccaacat cacgatgccg acgacgcagc cgagcgcggt
      301 ttcgcagaac tctgtccagg tgaagccagg gctaaccaac aagagggcgg gcgagtagac
      361 gaagacaaat ggcaccaagg ccttggcgag gccaaggcgg aaagccgtat tgccggtctt
      421 gaagggatcc gcgcccgcca tgctcgcacc ggcataggcg gcaagggcca ccgggggcgt
      481 aatgtcggcc agcacgccat agtagaagac gaagaagtgc gcgatcagcg gcgctacgcc
      541 catgagggcg agggcaggtg cggccacggt caccatgatg atgtacgtgg gcgtcgtcgg
      601 cacgccgcag cccagcagga tgcagacaat cgcggtcaag acgagggtgt aaaacaaggt
      661 gagccccttg ggctcaaaca gtgcgaccgg cagcatactg ccggtgacgg ccgcgaggtc
      721 agccgctgtg ctggttatga tggcagagag cttgaagcct ataccggtca gggtcacgac
      781 gccgacgatg atgccgacgg cggccgccgc cgcaccgacg ctgatggcgt atttggcccc
      841 ggtgacaaaa gcgtcgagga cttcgagaag acgctgctgg ccgccagcat ccttggccac
      901 gtagaacgcc gataccgcgc cgcaagcgcc gagaatgacg gctacccctt gccattcagt
      961 gagctcaccg gcgagttcgg ccgcggcgat ggcgcccagc atgccgaggg cgacgatcgg
     1021 actgcgattg ctgacgccga tcacaattga ggccgtgatg ccccagaacg ccgccaggta
     1081 gggcgtaaat cccgagaaca ggacgatcag cagcacgagc aaaggtatgg aggttggcca
     1141 gtcgcgtcgc aggatctcgc cgatcctcgg catctcggcg gcgctcatgc cgcgcatgcc
     1201 gcgcttcttg gcctcgaagt ggacctgaca aaagacgccg aaaaaatgca tgaaggcggg
     1261 aacaatggcc gcgatgatga tggtctgata gggaattgag aggtactcca ccatcaaaaa
     1321 ggccgccgcc cccatgatgg gcggggtaat ttggccgccc gtcgccgccg tcgattccac
     1381 cgcgccggcg aagtgacgcg ggtagccgat gcgaatcatc atggggatgg tcaaggagcc
     1441 gaccgtcacc gtgttggcga tcgagctgcc tgagatcatg ccaaataggc ccgaggcaaa
     1501 gatggaaact ttcgctggtc cacccgcgaa gcgcccagca atggccgtgg caatgtcgat
     1561 gaaaaagcga ccgagtccga cgcgcgtcgc caacaccccg aacaagacat aatggaagac
     1621 gtaggtcgca accaccccca aggcgatgcc gtagaccccg ttcaccgtta aatagaggtg
     1681 gttgacgacg ctcttccagc tattgcccgg gtgcgcaaag atgccgggca gggaaggtcc
     1741 tagcagcgca tagataatga gcacgacggc aatgatcgac agagcggggc cgacgctacg
     1801 ccgcgtcgcc tccaagacga cgataagcat cagtgttccc atgatccagt cgatcgggtc
     1861 gggattgccg acccggaact gcagatcgtg gaaaacccag ggcacataga ggctggcgag
     1921 cgcggcagcg atcgccagca gccagtcagc aagtccgatg ccgagcgggc gcaaccggct
     1981 cgagcgcggc aaggacgcgc tattgtttcg ccgcgctgga aaaaccagaa agatcaaccc
     2041 cagcacgaag gcaacatgga tgccgcgatg gatcacctcg ggcagcagac caaagccggc
     2101 ggtgtagtaa tgaaagcagg aaagtgtgaa gagcagtgcc gccaccaacc atgcagctcg
     2161 cggtagcaac ggacggaact gcatttccgg gtcgtgttcg gcctcgagct tgtcaaggtc
     2221 ggcgggcagc tgcgacagcg cgttcgttga ggtcgtggac atgtggcgcg gatgccttgc
     2281 aagagccaac tcaaagatcg cgcaacctgg gccgccgcga cgggcgaccg atgcaaggcg
     2341 caatcaggct tggcctcact tgagcattcc ggcttcccgg tagaatttct cggcacccgc
     2401 gtgtagcgga atgccgagcc cgttgagcgc cgtctccttg cgtatgacct tgcccttggc
     2461 gtggcccgcg tcgagcgcag ccctggtttt gtcgctccac atcgccttgg tcacggcata
     2521 aacgaggccc tcctccaatt tagccgaggt cacccactgg gccccgacgg agagcgtttt
     2581 gacggcgggc acgtctttgt agaccgcggc agggatctca tcggccgcga agaatttgaa
     2641 gcgagcttgc aattgctccg cttcgggacc tgccaggggc aacagctcga aaccgttggt
     2701 tgcggccaat tccgaaagtg tgccctgcgg gtagccggtg gtctggaaat aagcgtcgag
     2761 cgagccatcc ttcagctttt cggcacactg ctgctgctta agatattccg gcttgatgtc
     2821 cttctcactc aggccatagg cggcgagaat ggctttggcg ttgacgagcg tgccggaacc
     2881 tggctcgtca agggagacgc gcttgcccct gaggtcggcg acggacttgg cgcccaaccc
     2941 cttcttgacc acgatgtgca cgctttccgg ataaaggttg gcgatggcgc gcagctcttc
     3001 caccttgggc ttgccctcgt aaattcccgt gcccgtgtag gcccagaagt tgacatcagc
     3061 ctggctgaaa ccggactcca ttgcgccggc aatgatgccg ttgacattgg cgaccgaacc
     3121 gttagtggcg acggcgctga cattgaggtt ctccgaggag atggcgttgc cgatcaggcc
     3181 gccaatcgga tagtaggttc cgcccgtgcc gcccgtgccg atccggaaga acctttgctg
     3241 tgcaacggcc ggtccatgta acagggccgc ccctgccagg ccggcgccga gcctcagcac
     3301 ctggcggcgc gtcgttgtcg tcattgctcc cattccctca cgtcatgcag gctcggccga
     3361 ttggccgcgc atggctcgca acctagacca aacagaccct cgccgtgaag acggcacgcg
     3421 cccacgctcc gctgccattg gggcatactt gcgtgtgggc tgggaggcgt tggctaatcc
     3481 tgcgctcggc tcgcctcgac cgccacggcc aattcctgca agaggcgcgg ggcgaccttg
     3541 gcggaggccg gacaatccgg cgacttcaag cgcgcgtgga tcgccgccac ctcgcctttg
     3601 aagccgtccc caagtgcgcg cttggcaatg ccggccgcca ccgcgccgtc cgcccagcgc
     3661 gattggcaca tcaaccgcac gagatagccc gtcaggcggg acttatactg gtcggcatcg
     3721 ctcgcctcgc ggggcttgag gaggacctgc catgcctggt gctcgggcga agcgctccag
     3781 gcccgactct cggcggcaag gcgcacctct aggcttgcgt catcggtgcg cgagggcacg
     3841 ctgagctggg ccagatcctg atcacgcggc ggtcgcagga cgatctcggc gaagtcggcc
     3901 agcgccaccg cgtcgttagt cggtggcacc gtccgccaga ccagcgcagc cgtcaggtcg
     3961 gcgccgacga gccgtgcggt ggcaaggtcg gcggcaaaga ggatgctgcg cgtaaggttg
     4021 gcaccgtcca gctcggcctg ccgcaagcgt gccccttcaa ggcctgccat cgacaggtcg
     4081 gcgccttgca acctggcgtt gctcaaattg gcgagatgga gttgcgcccc cgaaagatcg
     4141 gccccctgca gccacgcgcc gaccgcgttg gccccccgca agacggcgcc ggcgaggttg
     4201 gcacgctgca gccgggcgcc gctgaggttg gcgttcgtga gcgcagcacc gtt
//
LOCUS       DASTMT010000532         4185 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_3254943, whole genome shotgun sequence.
ACCESSION   DASTMT010000532 DASTMT010000000
VERSION     DASTMT010000532.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4185)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4185
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3254943"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(92..166)
                     /locus_tag="VFR00_12225"
     tRNA            complement(92..166)
                     /locus_tag="VFR00_12225"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(132..134),aa:Gln,seq:ttg)
     gene            complement(241..1203)
                     /locus_tag="VFR00_12230"
     CDS             complement(241..1203)
                     /locus_tag="VFR00_12230"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HEU0160080.1"
                     /translation="MANRINRAIELLAQDQAIYYVGQHTGHDLSISQGRLDARTWADY
                     INIGMEHGAFDMAGLAEYMRGLVDAGPTNSGHRTPAVIVEVPVRGTDVAGVRSNTWQF
                     RQILARGVHGLLLCQAESAEAVRVFVEATRYPHHCLGVDASLPSPVARMHGEVIRAEG
                     PQRPLGPPLLAVGSRGRGSEATAAAVWGLSAADYMERCDPWPLNPAGELLLGVKIESP
                     EGVANCESILEVPGLGFAEMGPGDLGLALGYPLVPREPYPPEMQEARRRVFTACKRNR
                     VAFLEGCSPATIASKLDEGVRVIAGQDPATAVIGRAHQKRSLPV"
     gene            1342..2490
                     /locus_tag="VFR00_12235"
     CDS             1342..2490
                     /locus_tag="VFR00_12235"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012319384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEU0160081.1"
                     /translation="MALATAQLSPYVPPRAERDWEEVSAAGAGFEATKLRAAIDFARS
                     NDSPWPRSLYYPDGRYVGIVEWNETGPWSEVAGPVEARGGPAGLILKGGRIVAEWGDT
                     ARTDMTFSIAKSYLAVIAGLAVADGLIDDVDAPVAASIAGPFFASPHNACITWRHLLQ
                     QSSEWRGELWGKSDQVDHHRQVGAGADNARKGVRRDLETPGSYYEYNDVRVNLLAFCL
                     LQRFGRALPEVLRERIMDPIGASSTWQWHGYRTSWTDVAGRRVQSVSGGSHWGGGMFI
                     SARDHARFGLLIARGGAWQGRQLIPSAWVEAMLSPSPTNEGYGYLWWLNRGRARHPSA
                     PTSSVFALGAGSNIIWIDPEHDLVSVLRWIDKAAVDAFLARLLAAIGG"
     gene            complement(2496..3449)
                     /locus_tag="VFR00_12240"
     CDS             complement(2496..3449)
                     /locus_tag="VFR00_12240"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03619 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HEU0160082.1"
                     /translation="MKFGLSTLTRGLLTTREAYQAVAKAAEAAGFDFLSVSDHIVVPA
                     ELKSAYPYAVGGVFGAAEHGHCLDQLATIAFLAGSTERLRLLTSVLVVPHRPAILAAK
                     LLATIDVLSKGRLIVGAGAGWMREEFALLDLPFEARGSVTDEYLEAFRELWTKDRPAY
                     SGKHVRFAEVLFYPKPLQQPHPPIWIGGESPAALKRAIRLADAWYPGNNNQRRPLDTP
                     ARLAAAVAALRVDCEQAGRDPRQLGISLLVQDPFEWSARKTQDGSGRRLFTGSSDEMV
                     GDAGALAAIGVGHVAVRLGGQSIADAVARIERFGAEVIAKL"
     gene            complement(3504..3908)
                     /locus_tag="VFR00_12245"
     CDS             complement(3504..3908)
                     /locus_tag="VFR00_12245"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEU0160083.1"
                     /translation="MTASELYKKGEAVRRRLRGDADFERNKREYERDPVMQKFIEVAT
                     ETVFGALWTRPGLDIKTRTLICVISDAATGRHPELDIHLRFALKEGWTENELTEALLH
                     LSGYVGVPIIREAMLVAAKVFAEVRAARSGTG"
BASE COUNT          713 a         1374 c         1356 g          742 t
ORIGIN      
        1 cggctcatga gaagagagcg tcctgcggcg gaacggagtt gcattcgcag cgcaaatgat
       61 cgtttgcagg gtcgcttaga ccgaagcgct ttggctgggg cgggaggatt cgaacctccg
      121 catggcggaa ccaaaatccg ctgccttacc gcttggctac gccccatcga cggcttgcct
      181 tctacgcgca acggcatggg gttgcaaggc tgcacccgcg caaaaagtgg cgctttggca
      241 ctacactggg agggagcgct tctggtgcgc gcgtccgata acggcggtgg cgggatcttg
      301 tccggcgata acgcggacgc cctcgtcgag cttactcgcg atagtggcag gcgagcaccc
      361 ctcgagaaag gcgacccggt ttcttttgca ggcggtaaac acgcgccgcc gcgcctcctg
      421 catctcgggt ggataaggtt cacgcggcac aagcgggtag ccgagagcca aaccgagatc
      481 acccggtccc atctcggcaa agccgaggcc cggcacctcc agtattgact cgcaattggc
      541 cacgccttcg gggctctcga ttttgacccc cagcagcaat tcgccggcgg gattgagcgg
      601 ccatggatcg caacgctcca tgtagtccgc cgccgatagg ccccagacgg cggcggcggt
      661 ggcctccgag ccgcggccgc ggctgccgac ggcaagcaga ggcgggccca gggggcgctg
      721 gggtccctcg gctcggatga cctccccgtg catgcgcgcg accggggatg gcaggctcgc
      781 atccacgccc aagcagtgat gcggatagcg cgtcgcctcc acgaacacgc gcaccgcctc
      841 cgccgactcg gcctgacaca gcaacaggcc gtgcacaccg cgcgcgagaa tttgccgaaa
      901 ctgccaggta ttgctgcgga cgccagccac gtcggttccc cgcaccggca cctcgacgat
      961 tacggcgggc gtgcgatggc cggaattggt tggcccagca tcgacgagac cacgcatata
     1021 ctcggccagg cccgccatgt cgaatgcgcc atgctccatg ccgatgttga tgtagtcggc
     1081 ccaggtccgg gcatccaggc gcccctggga aatgctcaga tcgtgcccag tgtgctggcc
     1141 cacgtagtag atggcctgat cctgggccag aagttcgatg gctcgattga tgcggttcgc
     1201 catgcctacc ttcctcattt cgggctgcct caggcagcgc gccgccccac ccgctcggcc
     1261 aagcgagtat aggcgctttg ccgggggcag gtcgccgtga taaggcccca ggccgagacc
     1321 acgaaaataa gggagcgcgc gatggcgctg gcaactgcac aactttcgcc ctacgtgccg
     1381 ccccgcgcag agcgcgactg ggaagaggtt agcgccgccg gagcaggctt cgaggccacg
     1441 aaattgcggg ctgccattga ctttgcgcgc agcaacgaca gcccatggcc gcgcagcctt
     1501 tattatcccg acgggcgcta cgtcggcatc gtcgaatgga acgaaaccgg gccctggagc
     1561 gaagttgccg gccctgttga ggcacgcggc gggccggccg ggctgatcct caagggcggg
     1621 cgcatcgtgg ccgaatgggg cgacaccgcc cgcacggaca tgacctttag catcgccaag
     1681 agctacctgg ccgtgattgc cggtctcgcc gtagccgacg gtctgattga cgacgtagac
     1741 gccccggtag cggccagcat tgccggcccg ttctttgcca gtccgcacaa cgcgtgcatt
     1801 acctggcgcc acctgctgca gcaatcgagc gaatggcggg gtgagttgtg gggtaagtcg
     1861 gatcaggtcg atcaccatcg ccaagtcggc gccggggccg acaatgctcg caagggcgtc
     1921 cgccgtgacc tggagacacc cggcagctac tacgagtaca acgatgtgcg cgtcaatttg
     1981 ttggcgttct gtttgctgca gcgttttggt cgagcgctgc ccgaggttct gcgcgagcgc
     2041 atcatggacc cgatcggtgc ctcgagcacc tggcaatggc acggctaccg cacgtcctgg
     2101 accgacgtcg caggccgacg ggtgcagtcg gtctcgggcg gttcgcattg gggcggcggc
     2161 atgttcattt cggcgcgcga tcacgcccgc ttcggtctat tgattgcgcg cggcggggcg
     2221 tggcagggcc gccagctcat tccctccgcg tgggttgagg ccatgctttc gccctcgccc
     2281 accaacgagg gctatggcta tctatggtgg ttgaaccgcg gccgggcgcg acacccgagc
     2341 gctcccacca gcagcgtatt tgcgctcggg gccggctcca atatcatctg gattgatccc
     2401 gagcatgatc ttgttagcgt gctgcgctgg atcgacaagg ccgcggtcga cgcctttttg
     2461 gcccggttgc tggcggcaat cggcgggtag cggcttcaaa gcttggcgat cacctcggcc
     2521 ccgaagcgct cgatgcgtgc cacggcatct gcaatcgact gaccaccgag ccggaccgcg
     2581 acatgaccga ccccgatggc cgccagcgcg ccagcgtcgc cgaccatctc gtcggaagaa
     2641 ccggtaaaca ggcgccggcc gctgccgtct tgggtcttgc gtgcactcca ttcgaagggg
     2701 tcttgcacga gcagcgaaat gccgagctgc ctgggatcgc gtccagcttg ttcgcaatcg
     2761 acccggagcg ccgcgacggc ggcggcaaga cgcgccggcg tgtcgagggg acgtcgctga
     2821 ttgttgttgc cgggatacca agcatcggcg agcctgatgg cacgcttgag agcggcgggg
     2881 ctttctccgc cgatccagat gggcggatgg ggttgctgga ggggcttggg gtagaacaga
     2941 acctcggcga aacgcacatg cttgcccgaa taggctggtc tgtccttggt ccagagctcg
     3001 cgaaaggcct ccagatactc gtcggtgacg cttccccgcg cctcgaaagg caggtcgagt
     3061 aaagcgaact cttcccgcat ccagcctgcc ccggcaccca cgatcaggcg tcccttggac
     3121 aatacatcga tggtcgccag cagtttggcc gcgagtattg ctggacgatg cggcacgacc
     3181 agcaccgagg tcagcaagcg cagccgttcg gtcgaccccg ccaggaaggc gatggtggcg
     3241 agctggtcga ggcagtgtcc gtgctcggcg gcgccgaaca caccgcccac agcgtagggg
     3301 taggccgact tgagctcggc cggcacgacg atgtggtcgc ttaccgagag aaagtcgaaa
     3361 cctgccgcct cggcggcctt ggcaacggcc tggtaggctt cacgcgtcgt caacaggccg
     3421 cgcgtgagcg tgctcagtcc aaatttcatg gtgctccgcc tttcctcagc cttcgccctt
     3481 cgcctttgcc cgggtgccgc gccctagccc gtgcccgacc gcgcggccct gacctcggcg
     3541 aacacctttg ctgcgaccag catggcttca cggataatcg gcacaccgac gtagcctgaa
     3601 agatgcagga gggcctcggt cagctcgttt tccgtccagc cctctttcag ggcaaagcgc
     3661 aggtggatat cgagttccgg atgccgcccg gtcgcggcat cggaaatgac gcaaatcagc
     3721 gtgcgtgtct taatgtcgag gcccggcctg gtccacagcg cgccgaatac ggtttccgtc
     3781 gccacctcaa taaatttctg catcaccggg tcacgctcgt attcgcgctt gttgcgctcg
     3841 aagtccgcat caccccggag cctgcggcgc acggcttcac ccttcttgta gagctcgctt
     3901 gcggtcatga cggcatttcc cctttgctct ctggctgtcg ctgcctcgcc ccgccggctt
     3961 ggctcagcgc aacgccggtg ggtatgaggc tggtgcggca aggctagccc agcggcggcg
     4021 gcaggcatag ccggagcctc cgccgcgcgc ccgatgctca gccatttgcc ttattgcgcc
     4081 gcaggagacc gcatttgcgc gacccatcgg cctcgggcat acttatccgt gtccgttcgt
     4141 cggtagccaa aaccagatcc tcaatctggc cacctgccga cgtcg
//
LOCUS       DASTMT010000533         6129 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_5402822, whole genome shotgun sequence.
ACCESSION   DASTMT010000533 DASTMT010000000
VERSION     DASTMT010000533.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 6129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6129
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5402822"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..737)
                     /locus_tag="VFR00_12250"
     CDS             complement(<1..737)
                     /locus_tag="VFR00_12250"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0160084.1"
                     /translation="MKLEANNLKVQLQRRQVLNGVDLSAASGELTAVLGANGAGKTTL
                     LRVLAGLIPAAAGEVRLDGRPLGTWGRNDLARSLAYLPQQRTVHWALSARNVVALGRL
                     PHQAMGQRETTTDHAAIERAIAAMDIAPLAGRPVLAMSGGEQARVLLARALAQEPHVL
                     VADEPAAGLDPAHQLALFHHFAAFAASGRTVLLALHDLSLAARFCHKIVLLNGGRALA
                     AGPPATVLTRPHLATAYAIRAEYRLIDG"
     gene            complement(734..1699)
                     /locus_tag="VFR00_12255"
     CDS             complement(734..1699)
                     /locus_tag="VFR00_12255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007007508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEU0160085.1"
                     /translation="MAGRLVAGLAGLVLFAFVLSLALGPSGFGFGPAGEAGSLIFWEI
                     RLPRALLGMLVGATLGLSGSALQGYLRNPLAEPSLIGISGGAALGAVLTIHLGIAQTL
                     ALALPIGGLLGAAIAMLGVLALAGIRGGPITLILAGLAVSATATALISLALNLSQNPF
                     ASIEMVYWMMGSLADRSLTQVWLSGPLMLVGMGLLLLVGRALDALTLGEDAAVSLGID
                     ARRTRLAIVAGTALSVGAATAVTGIIGFVGLLVPHALRPLIGHRPGLLLPASLFGGAI
                     LVLSADIALRLIQPWADVRIGVLTALFGAPFFIWLVLKTRSELAP"
     gene            complement(1720..2568)
                     /locus_tag="VFR00_12260"
     CDS             complement(1720..2568)
                     /locus_tag="VFR00_12260"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0160086.1"
                     /translation="MQVSARLGLLVAVFLAAAALARAEPPRRIVSLDLCTDQLLIELA
                     PPERIAAVTHLATDASVSAIPQKAKGIPITHGAAEDVLGYDPDLILAGPFGVSSTVNL
                     LRRLNRNVVIVPLAQDLDGVRASIRAIAIAIDEPRRGETMVKAFDQRLTALSRPHDGA
                     VLTAIVYQVGGIVSASGSLADAALSASGFHNLARDYPLSRSGQLPLELLVASPPDLLV
                     LSTASTGYRTTAADNLRHPALDKLRRTHASLELPWQLWLCGTPHIVDAIEQLSTARER
                     LRAAPR"
     gene            complement(2630..4711)
                     /locus_tag="VFR00_12265"
     CDS             complement(2630..4711)
                     /locus_tag="VFR00_12265"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU0160087.1"
                     /translation="MRLSPIPGFPAAAAALALQFLSSSASAQQSVALPGITVQGATLE
                     RGRVQAPSSPTAPPPSSQAAADATAADATALGVPIETIGNAVTVITGEDLRRQQVRSA
                     ADALRGLPGVAVNRQGGVGSITQVRIRGAEANHTLVLIDGIEANNSTDGEFDFSNLSA
                     EDIERIEVIRGPMSSLHGSNAVGGVINIITRRGRGPMTASLRAETGSFATNDIVARLS
                     GGNDRANLSISAQRQRTDGFNIAPVGGEKDGSERNTLSLRAGIKLMENMALDLSLRNM
                     EKRADRDGFGDFTAPAGSFAQAFDDKSTLANHVFLAGANLKWDMLSGALSHEFRANHN
                     GTTTQDSDRTFFSESKNISTTDTFAYAGTYRFDTPAIWAKHSLSALVERQDDQFTPEG
                     TFADGRTRERGRMSYAGEWRGGFADRLYLTAGIRRDDNEEFRDTTTWRLAASLPLSEL
                     GMRPHASVGTAVKLPTMFEQFGIDQFFSPNPDLKPEKSFGWDAGIEFTLPGSRASLDV
                     TYFNTDLTDKINGFVAIPGTFPQRFTAQNFIGTSTREGVEIATRVRLASNLSWGAAYT
                     YTNARDPDGREEARRPPHSARTDLSYTFANGRGTADLGVIYNGRMEDIAFLIPFFDQS
                     RVGLDPYWVVNAAASYKLQPGVELFARVENLLDQKYQEAFGFDAAPIAAFAGVKLTLG
                     GPDGLGGSWGK"
     regulatory      complement(4925..5135)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(5239..5757)
                     /locus_tag="VFR00_12270"
     CDS             complement(5239..5757)
                     /locus_tag="VFR00_12270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008598649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HEU0160088.1"
                     /translation="MKTERQAVAMLGAVAKGALAKGALALGVLLAVCAGPALAQELRT
                     YGKKGSYEDVKFELNDAIIRRGLAIDYTGHINKMLERTGADVGSTKHVYANAEFFMFC
                     SAKLSRDMIEADPTNLGYCPYVVFLYEAADKPGEIVVGYRRPLLKGDAQSQKALGAID
                     ALLDGIVKEAVK"
     gene            complement(5832..6032)
                     /locus_tag="VFR00_12275"
     CDS             complement(5832..6032)
                     /locus_tag="VFR00_12275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0160089.1"
                     /translation="MAQQSQTAAGPRAPRRARIERMPRALLTALLALLGALLAGAAYL
                     LSVRGEALILDLAAMSQRIFCF"
BASE COUNT         1122 a         1865 c         2109 g         1033 t
ORIGIN      
        1 ccatcaatta ggcgatactc ggcgcggatg gcgtaggcgg tggccaggtg ggggcgcgtg
       61 aggactgtcg ccggcggccc cgcggccagg gcgcggccgc cgttgagcag cacgattttg
      121 tgacaaaagc gcgcggccag cgacaaatcg tgcagggcca gaagaacggt gcggccgctg
      181 gccgcaaagg cggcaaagtg atggaacagt gcgagctggt gcgccggatc gaggccggcg
      241 gcgggctcgt cggcaacgag gacgtgtggt tcctgcgcca gggcccgcgc taagagtacg
      301 cgggcctgct cgccacccga catggccaag accgggcggc ccgccaacgg cgcaatgtcc
      361 atggcggcga tggcgcgctc gatggccgcg tggtcggtgg tggtttcgcg ttgccccatg
      421 gcctggtgcg gtaagcggcc gagggccaca acgttgcgcg ccgagagcgc ccagtgcacg
      481 gtgcgctgct gcggcaggta agccaggctg cgggcaagat cgttgcgccc ccaggtgccg
      541 agcgggcggc catcgagccg cacctcaccg gcggcggccg gaatgaggcc ggccaagacc
      601 ctgagcagcg tggtcttgcc cgcgccgttg gccccgagca cggcggtgag ctcgcccgac
      661 gccgccgaga ggtcgacacc gttgaggacc tggcgtcgct gcaactggac cttgaggttg
      721 ttggcctcga gcttcatggc gccagctcgc tgcgggtctt gaggaccagc cagatgaaaa
      781 acggggcacc aaagagcgcg gtcagcacgc cgatgcgcac gtccgcccac ggctgaatga
      841 gacgcagggc gatgtcggcg ctgagcacca ggatcgcacc gccgaacagg cttgccggca
      901 gcaagagacc gggacgatgg ccgatcaggg gacgcagcgc atgcggtaca agcaaaccaa
      961 caaagccgat gataccggtg acagcagtgg cggcgccgac gctgagcgcg gtgcccgcca
     1021 cgatcgcgag ccgcgtgcga cgggcatcga ttccgaggct gaccgcagcg tcctcgccca
     1081 atgtcagggc atcgagtgcc cggccaacga ggagcaagag gcccatgccg acgagcatca
     1141 agggcccgct cagccacacc tgtgtgaggc tccggtcggc gagcgagccc atcatccagt
     1201 acaccatctc gatggaggcg aaggggttct gcgacaggtt gagggcaagc gagatcagcg
     1261 ccgtcgcagt ggcggaaacg gcgaggccgg ctagaatgag ggtgataggc ccgccgcgga
     1321 tgccggcgag tgctaacacg cccagcatgg ctatcgcggc gcccagcagt ccgccgatcg
     1381 gcagcgccaa tgcaagtgtc tgggcgatgc cgagatgaat ggtgagcacg gcgccgagcg
     1441 cggcgccgcc cgagatgcca atcaggctag gctcggcgag cggattgcgc agatagccct
     1501 gcagggcgct gccggagagg ccaagcgtag cgccaacgag catgcccaag agggcgcgcg
     1561 gcaggcgaat ctcccaaaaa atcagcgaac ccgcctcgcc ggcggggccg aagccgaagc
     1621 cggagggccc tagggcgagc gaaaggacga aggcgaagag gactagtccc gccaagcccg
     1681 cgacaagtcg tcctgccaca tttgttgcgg ctgggctgct catcgcggtg cagcccgcaa
     1741 gcgttcccgc gcggtggaaa gctgttcgat ggcatcgacg atatgcggcg ttccgcacaa
     1801 ccaaagttgc catggtagtt cgagggaggc atgcgtgcga cgcagtttgt cgagcgctgg
     1861 atggcgaagg ttgtccgcgg ctgtcgtgcg gtatccggtg ctcgcggtgg aaagaacgag
     1921 gaggtcggga ggtgatgcca ccagcaactc gagaggtagc tgtccgctgc ggctcaatgg
     1981 gtagtcgcgc gcgaggttat ggaagccgct cgctgagagc gcggcatcgg cgaggctgcc
     2041 gctcgccgac actatgccgc caacttggta gacgatggcc gtcaggacgg cgccgtcgtg
     2101 tgggcggctg agggcagtca ggcgttgatc gaacgccttg accatggtct ccccgcgcct
     2161 gggctcgtcg atggcgatgg caatggcgcg tatcgacgcg cgcacgccat ctagatcctg
     2221 cgccagcggc acaatgacga cgtttcgatt aagccggcgc agaaggttga cagtggacga
     2281 gacgccaaac ggtcctgcaa gaatgaggtc ggggtcgtag ccgagtacgt cctcggcggc
     2341 cccatgcgta atgggaatgc ctttggcctt ttgggggatg gcggagacag aggcatccgt
     2401 cgccaggtgg gtgacggcgg caatgcgttc aggcggcgcc agctcgatca acagttggtc
     2461 cgtgcacaaa tcgagcgaaa cgatgcgtcg cgggggctcg gcgcgtgcca gcgccgcggc
     2521 ggccaagaag acagcgacca agagacccag gcgggcggac acctgcacgc cgttgcggac
     2581 ccctaccgcc agccaccgtg gaatcgtctt gccgccgcgc ttgagcgctc tatttgcccc
     2641 aactaccacc aagcccatct ggtcctccga gcgtcagctt gacgccggca aacgctgcaa
     2701 tcggcgcagc gtcgaagcca aaggcctctt gatacttctg atcgagcaga ttctctacgc
     2761 gcgcgaacag ctcgacacct ggctgcagct tgtaggaggc ggcggcattg acgacccagt
     2821 agggatcaag cccgacgcgc gattggtcga agaaagggat caagaacgca atatcctcca
     2881 tgcgtccgtt atagatgaca ccgaggtcgg cggtgccacg gccattggca aaggtatagc
     2941 tgagatcggt acgggccgag tggggcggcc gccgcgcctc ttcgcggccg tcggggtcgc
     3001 gggcgttggt gtaggtgtag gcggcgcccc aactcaggtt gctagcgagc ctcacacgcg
     3061 tggcgatttc cacgccttcc cgcgtgctcg tgccgatgaa gttttgcgcg gtaaagcgct
     3121 gggggaaggt accgggtatg gcgacgaaac cattgatctt gtcagtcaga tcggtgttga
     3181 aataggtgac atcgaggctg gcccgactgc ctggtagggt gaactcgata cccgcgtccc
     3241 agccgaagct tttttcgggt ttcagatcgg ggttggggga gaaaaactga tcgatgccga
     3301 actgctcgaa catggtcggc aatttgacgg ccgttcctac gctggcgtgc gggcgcatcc
     3361 ccaattcgga gagcggcaag gatgctgcca agcgccaggt cgtggtatcg cggaattcct
     3421 cgttgtcgtc gcggcgaatc ccggcggtca agtacaaacg gtcggcaaat ccgccgcgcc
     3481 actcgccggc gtaggacatg cggccgcgct cgcgcgtgcg tccgtcggca aatgtgccct
     3541 cgggcgtaaa ttgatcatcc tgcctttcaa ccagtgcgga gaggctgtgc ttggcccaga
     3601 ttgccggggt gtcgaagcga taggtgccgg cgtaggcgaa cgtgtcagtt gtgctgatgt
     3661 ttttgctttc ggagaagaac gtgcgatcgc tgtcttgggt ggtggtgcca ttgtggttgg
     3721 cgcggaactc atgggaaagg gcgccgctca gcatgtccca tttgagattg gcgccggcca
     3781 ggaagacgtg gttggcgagg gtcgacttat cgtcgaaggc ctgcgcaaac gaaccggccg
     3841 gggcagtgaa atctccgaat ccgtcacgat cggcgcgctt ttccatgttg cgcaacgaga
     3901 ggtcgagggc catgttctcc atcagtttga tgcccgccct gagcgacagg gtgttgcgct
     3961 cggaaccgtc cttttcaccg ccgacgggag caatattgaa gccgtcggtc cgttggcgtt
     4021 gcgcgctgat cgacaggttg gcacgatcgt tgccgccaga cagacgtgcc acgatatcat
     4081 tggttgcaaa tgatccagtc tcggcccgca aagaagccgt catgggcccg cggccgcgac
     4141 gcgtgataat gttgatgacc ccgccgacgg cgttggagcc gtgcaggctg ctcatggggc
     4201 cgcggattac ctcaatgcgc tcgatgtctt ctgccgatag gttggagaag tcgaactcgc
     4261 catcggtgct attgttggcc tcgatcccgt cgatgagcac cagtgtatga ttggcttccg
     4321 cgccgcgaat gcggacctgc gtgatgctgc ccacaccgcc ctggcgattg acggcgacgc
     4381 ccggtagccc gcgcagggca tcggcagcgc tgcgcacctg ctggcggcgc aaatcctcgc
     4441 ccgtgatgac ggtgacggca ttgccgatgg tctctattgg aacacccagt gccgttgcgt
     4501 cggcggcggt cgcatcggcg gccgcttggg aagaaggtgg aggagccgtg ggggatgacg
     4561 gtgcttgaac gcgaccgcgc tcaagtgtgg cgccctggac agtgataccg ggcagtgcca
     4621 ctgactgctg ggcggaggcg gagctggaca ggaattgcag ggccaaagcg gccgcagccg
     4681 cgggaaatcc tgggatcggt gacagacgca tgacggctct ccaatgtgag agtcgaaagg
     4741 cgcgtcagtt cgtcgatcca aaccaccagc aacacggcga gcatcagcgc cctgtcaaca
     4801 aggcgccgtc aactaaaccg ttgaatagga ttggcccttc acgcacaaca ccggcacgac
     4861 cctcaactcg attacagagt cgtcgccact acggtgatcc gttcgccggc gcgccccgcg
     4921 tcggtgatgg gcgactcgtg ccggcaggtc tcctggctca cggctacagg cgcaccactc
     4981 ccgccttccc gccgataggc tcggcagtgg cactcgggag cgatgctcgc cgcttacagt
     5041 tgcgggggca gccgcggctt tgagatgaat accctcgcac cgcgttccct tttcaccgtc
     5101 gcggcccaac tgggaccgca acgagaccgt cacgcgggcc agatcgaagc aacgcgcggc
     5161 gcggctgtca attgcttacg tgcacggaag atactttgtg cttgcgcgct ggcggaacgg
     5221 caacgctgcg ctcgcgcgct acttcacggc ttccttgacg atgccgtcga gcagcgcgtc
     5281 gatggcgccg agcgcctttt gcgattgggc gtcgcccttg agcagcggac ggcgatagcc
     5341 gacgacaatt tcgcccggct tatcggcggc ttcgtagaga aacaccacgt aggggcaata
     5401 gccgagattg gtggggtcgg cctcgatcat atcgcgggag agtttggccg agcagaacat
     5461 gaaaaactcg gcattggcat agacatgctt ggtcgacccg acatcggcgc cggtgcgctc
     5521 cagcatcttg ttgatgtgcc cggtatagtc gatggcgagg ccgcgcctga tgatggcatc
     5581 gttgagttcg aacttaacgt cctcgtagct gccctttttg ccataggttc tcagctcttg
     5641 cgccagggcc ggcccggcac agacggcgag caggacgccg agtgccagag ccccttttgc
     5701 cagagcccct ttcgccaccg cacccagcat cgccaccgcc tgcctttcgg ttttcaagtg
     5761 ttgccccgtc tggcgccggt tcgggtcaat caatgcatgg atcgcgggtt cctcaggcga
     5821 gccagcgctt tttaaaagca gaagatgcgc tgcgacatgg ccgcaagatc taggatcagg
     5881 gcctcgccgc gcacgctgag caagtaggcc gcacccgcca acagcgcgcc cagcagggcc
     5941 agcagtgcgg tcagcagcgc gcgcggcatc cgctcgatgc gcgcccgtcg tggcgctcgc
     6001 gggccggcgg ccgtttggct ttgctgtgcc atgcgtttcc ttcttggtag cggctagcgg
     6061 cgcgccttag cgccgcaacc catcgcctca atgcgtcgcc tgaggctcac gtcgccgcgg
     6121 ccagccgcg
//
LOCUS       DASTMT010000534         2944 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_5403198, whole genome shotgun sequence.
ACCESSION   DASTMT010000534 DASTMT010000000
VERSION     DASTMT010000534.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2944)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2944)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2944
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5403198"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..374)
                     /locus_tag="VFR00_12280"
     CDS             complement(<1..374)
                     /locus_tag="VFR00_12280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012044181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0160090.1"
                     /translation="MRNSKPSRRRFLATTAAASATMIAAPFVRTAGAAGKLSIGFWDH
                     WVPGANDATKSLVDAWAEKEKVDVQIDFITSQGNKLLLTTAAEAQARSGHDIIAMNTW
                     LPAEHAKLLEPVDDLMGDLIKQ"
     gene            complement(485..1234)
                     /locus_tag="VFR00_12285"
     CDS             complement(485..1234)
                     /locus_tag="VFR00_12285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008836651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HEU0160091.1"
                     /translation="MPLEAIEPRRLYRQIADQLRHLIDHGEYAVGSRLPAERVLADRL
                     GVSRPTVREALIALEVEGRLKIRVGSGIYVVEQQPAAVVPGNAAIEGPLELLRARAFV
                     ESAVAEEAAVRARTEDIERLDEILVEVDHQHSGDELIALDRRFHAAIAAILDNAVLVR
                     FVGQLFDQRINPYFEQLARHIENASTWKETHLEHVVIRNAIAARDPLAARAAMRNHLQ
                     QAHGRFSRDFGEPPLAAAETDLRPVRARVVA"
     gene            complement(1395..2156)
                     /locus_tag="VFR00_12290"
     CDS             complement(1395..2156)
                     /locus_tag="VFR00_12290"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HEU0160092.1"
                     /translation="MKPNRMKEKLARGEPALGCSVMFPSAQVVEMLGYAGFDWVLIDC
                     EHGSIGLADLELMAIAADAVGITPIARPRTNAASDIQSVMDRGVMGVQVPHVNTGEEA
                     RRAVAAVKFGPGAQRGLAVGTRPDRWGLGARLPHFTQAANQQSLVCVQLEHESALAHL
                     DELLAVEGIDVFFIGPSDLSQSMGFPGDPKAPAVARAIDQALARIVAAGRTAGMPAAS
                     ETVAEVKAKGCSYIYTHLPRLLGAGAGAFLSQGRG"
     gene            complement(2153..>2944)
                     /locus_tag="VFR00_12295"
     CDS             complement(2153..>2944)
                     /locus_tag="VFR00_12295"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEU0160093.1"
                     /translation="IFLRGALATVMLMFGVGATRARRPLTLLLEPMMLVRLGSSAAAT
                     VFIVICLRHLPLATVNTVLQVTPLAVTAGAALVFRERVSLARWLAALAGFLGVALIVR
                     PDAGSFAAGTYFVLVMLLFTTIRDLTTRGLAPDIPSIFVAAASAAAISVAGFAVAPFE
                     EAWHWPSPRAWLWLTLSAGCLFVATTFMIMALRTGEVSVVAPFRYVPVPLSILLGWWL
                     WGEMPDLFACLGIGLVLAAGLYLLKRERLSLTRTTITPIDRSPAA"
BASE COUNT          560 a          969 c          947 g          468 t
ORIGIN      
        1 ttctgcttga tgagatcgcc catcagatcg tcgacgggct cgaggagttt ggcgtgctcg
       61 gcgggcagcc acgtattcat cgcaataatg tcatggccgg atcttgcctg cgcttccgcg
      121 gcagtggtga gcagcagctt gttaccctgg gaggtaatga aatcgatctg gacgtcgacc
      181 ttttccttct cggcccaggc gtcgaccagg ctcttggttg catcattggc accgggcacc
      241 caatgatccc agaagccgat cgaaagcttg ccggccgcgc cagcggtacg cacgaacggc
      301 gcagcgatca tggtcgccga cgccgccgcg gtcgtcgcca ggaagcggcg gcggctaggt
      361 ttcgagtttc tcatttcgca gtcctccata tgaactcatt ttcagacgtg gcgtcgatgc
      421 ccctcgagta gtgttttagt ccgctcatgg cgacggccat aaaagcaagc ggtaccctcc
      481 atattcaggc tacgaccctc gctcggacgg gccgcaaatc ggtctcggcg gcggcaagcg
      541 gcggttcgcc gaaatcgcgc gagaaccgac catgcgcctg ctgcaggtga ttgcgcatgg
      601 ccgcgcgcgc cgccagggga tcacgcgccg caatggcatt gcgaatgacg acatgctcca
      661 gatgcgtctc cttccaggtc gacgcattct cgatatgacg cgcgagctgc tcgaaatagg
      721 gattgatgcg ctgatcgaac aattgcccga cgaagcgcac cagcacggcg ttgtcgagaa
      781 tggccgcgat cgcagcatgg aagcggcgat cgagtgcgat cagctcatcc ccggaatgtt
      841 gatgatcgac ctcgactagg atttcgtcca gccgttcgat atcctcggtg cgcgcccgca
      901 cggcggcctc ctcggccacc gcgctttcaa cgaaggcacg cgcccgcagc agctccaacg
      961 gtccctcgat ggctgcgttg ccggggacca cagcggccgg ctgttgctcg accacataaa
     1021 tgcccgagcc aactctgatt ttgagccgcc cctcgacttc cagggcgatc aacgcctccc
     1081 gcacggtcgg gcgcgacacg cccagccgat cggccagcac gcgctcggcc ggtaaacgac
     1141 tgccaacggc gtattcgcca tggtcgatca gatgacggag ctgatcggcg atttggcggt
     1201 agaggcggcg gggctctatg gcctcaaggg gcaatgaatt acctcggcgc gtttccttcg
     1261 tggcttgcgg atccgactct gcggtcagtt ggtcagacca tttgacaagt gcgtcggggc
     1321 gcctgtcaag agcgcagccc cgtgatggcc cctgtctcag tccaaagtct gagggggcac
     1381 gacggggccg cctgtcagcc tcggccctgg ctgagaaagg cccccgcgcc cgcaccgagc
     1441 aggcgcggca ggtgggtata gatgtagctg cagcccttcg ccttgacctc tgccacggtc
     1501 tcgctggccg ccggcatccc ggccgtgcga cccgccgcca caatgcgcgc aagcgcctga
     1561 tcaatggcgc gcgccacggc gggcgccttc ggatcgccgg gaaaacccat ggattgggag
     1621 aggtcggacg gaccgatgaa gaacacatcg atgccctcga cggccaatag ctcgtcgaga
     1681 tgggcaaggg cgctttcgtg ctcgagctgc acacaaacga gcgactgctg gttggccgct
     1741 tgggtgaagt ggggcaagcg ggcgccgagg ccccagcggt cgggccgcgt acccacggcg
     1801 aggccacgct gcgccccggg gccgaacttg accgccgcga ccgcgcgccg cgcctcctcg
     1861 cccgtattga cgtgcggcac ttgcacaccc atcacgccgc gatccatgac gctttgaatg
     1921 tcgctggcgg cgttggtccg aggccgggca atgggcgtta tgccgaccgc atcggcggcg
     1981 atcgccatca attccagatc ggccagcccg atactgccgt gctcacagtc gatcagcacc
     2041 caatcgaacc ccgcataacc cagcatctcc accacctgtg cggagggaaa catgaccgag
     2101 cagccgagcg ccggttcgcc gcgggccagc ttttctttca tgcggttcgg tttcatgcgg
     2161 cggggctcct gtcgatgggc gtaatcgtcg tgcgcgtcag gctgaggcgt tcgcgtttga
     2221 ggagatagag gcctgccgcg aggacgagcc caatccccaa acaggcgaaa agatcgggca
     2281 tctcgcccca caaccaccag cccagcagaa tcgacagcgg taccggcacg tagcggaacg
     2341 gcgccaccac cgacacctct cccgtacgca aggccattat catgaacgtg gtggcgacga
     2401 acagacagcc ggcggagagc gtcaaccaca gccacgcgcg cggcgagggc cagtgccacg
     2461 cctcctcgaa gggagccacg gcaaagccgg ccacgctgat ggcggcggcg ctggccgccg
     2521 ccacgaaaat ggagggaatg tcgggggcaa ggccgcgcgt cgtcaggtcg cgaatggtcg
     2581 taaacagcag catgaccagc acgaagtagg tgcccgctgc gaagctgccc gcgtcggggc
     2641 gcacgatcag cgccacgccg aggaaaccag caagggccgc cagccagcgc gccaaggaca
     2701 cgcgctcacg gaatacgagt gcggcgcccg cggtcacggc cagcggcgtg acctgcaaca
     2761 cggtgttgac ggtggcgagc ggcaagtggc gcagacaaat cacaatgaac accgtggccg
     2821 cggccgaact ccccaagcgc acgagcatca tgggctcgag caggagggtt agcggccggc
     2881 gcgcccgggt tgccccaacg ccgaacatca gcatcaccgt cgccagtgcg ccacgaagga
     2941 agat
//
LOCUS       DASTMT010000535         1950 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_1944883, whole genome shotgun sequence.
ACCESSION   DASTMT010000535 DASTMT010000000
VERSION     DASTMT010000535.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 1950)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1950)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1950
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1944883"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..593)
                     /locus_tag="VFR00_12300"
     CDS             complement(<1..593)
                     /locus_tag="VFR00_12300"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0160094.1"
                     /translation="MANLEAGAIPGMPIGERTVWRARPFVGLLAALLLLVVPLAAARA
                     ATPGSQLTWGVHVSLAPTWFDPAEMSGIITPYMVLYALHDALVKPMPGQPFAPSLAKS
                     WSQSEDGLVYEFVLREGAKFHDGAPVTAEDVKFSFERYRGAANKILKDRVASVETPDP
                     THVRFKLKQPWPDFLTFYTSATAAAWIVPKAYVERVG"
     gene            845..1744
                     /locus_tag="VFR00_12305"
     CDS             845..1744
                     /locus_tag="VFR00_12305"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEU0160095.1"
                     /translation="MPDDLSTELAELKAVAARQSGRFQRIADLAWPAAAQIAVNITVD
                     FDAMLLRRLNNEPPMELAKGEFGGRVGIWRLLELFTAHRVKATIFTPGRICELYPRAL
                     HAAAAAGHELADHMWEHRVPAEPELARDHLTKTTAALERVSGRRPVGSRSHYPPALLA
                     SEGYLYNSHGARSHRPYYVLDASGGASLLELPFHFAIDDAMFFSFAWYRSPNAGQRLS
                     DTDRVFDLWAAAFQQQYRQRGYLNICLHPFVSGRALRIAMLDRLLAHMKASPGVWFAT
                     CEEVARHCLAAHPAKPGAVAAAN"
     gene            1761..>1950
                     /locus_tag="VFR00_12310"
     CDS             1761..>1950
                     /locus_tag="VFR00_12310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0160096.1"
                     /translation="MPWKEGYTISNEKSLDDQEVRWPDGKRCALHILVDLSVARGPAG
                     IGARELASPTALFAVNDGL"
BASE COUNT          317 a          649 c          655 g          329 t
ORIGIN      
        1 tcgccgaccc gctcgacgta ggctttgggc acgatccagg ccgccgccgt ggcactggta
       61 tagaaggtca agaagtcggg ccagggttgc ttgagcttga agcggacgtg ggtgggatcg
      121 ggggtttcga cgctcgccac gcggtctttc aggatcttgt tggcggcgcc gcggtagcgc
      181 tcaaaggaaa acttcacgtc ttcggccgtg accggcgcgc cgtcgtgaaa tttcgcgccc
      241 tcgcgcagca cgaattcgta aacgaggccg tcctcggatt gcgaccagga tttggccagg
      301 ctcggtgcaa acggctggcc cggcatcggc ttcactaggg catcgtgcag ggcgtagagc
      361 accatatagg gcgtgatgat gcccgacatt tccgcgggat cgaaccaggt tggggccagc
      421 gaaacgtgca ccccccaggt gagctggctg ccgggggttg ccgcgcgcgc cgcggccagg
      481 ggcacaacca ggagaaggag cgcggcaagg aggccgacaa acggccgagc gcgccaaacc
      541 gtgcgctcgc cgataggcat gcccgggatt gccccggcct ctaggttagc catggcgttt
      601 tcccttcagg gcgcaccggg agggtacttg agcgatggtc cgcaccacgg caatgccgtg
      661 tgacgcggtc ctcccagcgc gacggagctt tcgttgttgc cggaagcctt tgcggcctcc
      721 agagtgggcc actctagtgc attcacgccg ccgcgcacag gtcaaagtcg ccgctaaggg
      781 ctcccacctt cgtcgtgctt gcctgtaaga tgacttgggc gtcgtgcctc aggggggcgg
      841 ccacatgccg gatgacctca gcaccgaact tgccgagctc aaagccgtcg ccgcacgaca
      901 gagcggccgc ttccagcgca tcgccgacct cgcctggccc gccgccgccc agatcgccgt
      961 caacatcacg gtggacttcg atgccatgct gctgcgccgg ctcaacaacg agccgccgat
     1021 ggagctggcc aagggcgagt tcggcgggcg ggtgggcatc tggcggctct tggagctgtt
     1081 cacggcccac cgcgtcaagg cgacgatttt cacgcccggg cgcatttgcg agctctaccc
     1141 gcgggcgctg cacgcggcag cggcggcggg ccacgagctt gccgaccaca tgtgggagca
     1201 tcgcgtgccg gcggagcccg agctggcgcg cgaccatctg accaagacga cggccgcact
     1261 cgagcgcgtc agcggacgcc gccccgtcgg ctcgcgcagc cattatccgc ccgcgctgct
     1321 ggccagcgag ggctatctct acaactcgca tggtgcccgc agccatcggc cctactacgt
     1381 actggacgcg agcggcggcg ccagcctgct cgagctgccg ttccacttcg ccatcgacga
     1441 tgccatgttt ttcagtttcg cgtggtatcg cagtcccaac gccgggcagc ggctgtccga
     1501 cacggatcgg gtattcgatt tgtgggcggc cgccttccag cagcagtatc ggcagcgggg
     1561 ctacctcaac atctgcttgc acccgttcgt ttcggggcgt gctttgcgca tcgccatgct
     1621 cgaccgcttg ctcgcacata tgaaggcatc gccgggcgtt tggtttgcca cctgcgaaga
     1681 ggttgcccgg cattgcctgg cggcccaccc ggccaagccg ggcgcggtcg ccgcggccaa
     1741 ctagcgcggg agggccgcac atgccgtgga aagaaggcta tacgatctcg aatgagaaaa
     1801 gcctcgacga tcaggaggtg cgctggcccg acggcaaacg ctgcgcgctg cacatcctcg
     1861 tcgatctcag cgtcgcgcgc ggtcctgccg gcattggggc acgcgagctc gccagcccga
     1921 ccgcgctgtt cgcggtcaac gatgggctcg
//
LOCUS       DASTMT010000536         5594 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_1947908, whole genome shotgun sequence.
ACCESSION   DASTMT010000536 DASTMT010000000
VERSION     DASTMT010000536.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 5594)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5594)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5594
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1947908"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(240..995)
                     /locus_tag="VFR00_12315"
     CDS             complement(240..995)
                     /locus_tag="VFR00_12315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009211418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU0160097.1"
                     /translation="MPDKVMLITGASRGIGAATARLAATRGYAVAINYATRGAAAEAV
                     AADVRKAGRKAMTMKADIAKPEEIARLFKAVDAEFGRLDAFFNNAGIVNQAAKFVDVA
                     PERLQQILAVNTTGAFLAAQEAVRRMSTRLGGQGGAIVNMSSLAAKLGGAGESTDYAV
                     SKGAIDTLTIGLAKELAGEGIRVNAVRPGLIDTDIQRDFGIGDRVAKYKDLVPMKRGG
                     SAQEVAEAVVWLCSDAASYVTGVLLDVSGGRGL"
     gene            complement(1150..1449)
                     /locus_tag="VFR00_12320"
     CDS             complement(1150..1449)
                     /locus_tag="VFR00_12320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0160098.1"
                     /translation="MGHHRIAILIAALGLLGNPARAHDWYPLECCSGQDCAPADTVEQ
                     RPDGSYFVTSRGLSAVIPPDYALWRRSPDGRIHVCIRKLRSGGEYLVCAFRGPGV"
     gene            complement(1608..2195)
                     /locus_tag="VFR00_12325"
     CDS             complement(1608..2195)
                     /locus_tag="VFR00_12325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3299 domain-containing protein"
                     /protein_id="HEU0160099.1"
                     /translation="MPRAFPVAILLGLLSVAGALFAPVLAGTGEAIMSLKWDQHVPPA
                     PPKPPKAFFSGRPGDPSGLAGPEHGVSTTQPMPEGRWMSAPAKSSASPPAVVAGLDGK
                     RVQIGGYVVPLDFDATRVKEFLLVPFVGACIHVPPPPPNQIVYVKTEGGIDVRGTFEP
                     VWVTGTLTVTPAFTGLAEAGYSLEAEKVEPRPLKD"
     gene            complement(2208..3467)
                     /locus_tag="VFR00_12330"
     CDS             complement(2208..3467)
                     /locus_tag="VFR00_12330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013418261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0160100.1"
                     /translation="MIVLRLALQSLRNRWLTALLTVLAIAVSVMLLLGVEKIRTGARQ
                     SFADTISGTDLIVGARSGSLNLLLYAVFRIGNATNNLSWQSYQDVRARPEVAWAVPLS
                     LGDGHHGYRVLGTTLDYFSHYRFRQTHGLAFRAGAPFSDLFDAVLGADVAAVLGYKLG
                     DPITISHGLGSVAFVEHEDKPFRVSGILEKTGTPVDRTVHVSLEAIEAIHVDWQSGGR
                     IPGQSVSADEVRRMDLTPKAITAALVGLKSKLATFRLQREINEYRAEPLSAILPGAAL
                     QELWGLVGIAETALTVVSAMVVATALLGMVTMILTTLNERRREMAILRSVGARARTIL
                     ALLMAEAGVLTATGIALGIVLLYIGLVALAPYLDRSYGLDLPIDAPKPAELMTLAAIW
                     AAGCLAGLLPALRAYRLSVADGMTVRT"
     gene            complement(3464..4204)
                     /locus_tag="VFR00_12335"
     CDS             complement(3464..4204)
                     /locus_tag="VFR00_12335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0160101.1"
                     /translation="MVALSRQAPAAAAHVLRVSDVRFTWPGRNGFALAIDRFALPAGA
                     RLLLIGPSGSGKSTFLGLLCGVLTPQAGSIEILGRDITRLSASARDAFRAEHFGIIFQ
                     MFNLLPYGTVLDNVLLPLSFAPGRRRRAAARAPVEQEAARLLGRLGLEAGLIRGPLAA
                     KLSVGQQQRVAAARALIGAPELIVADEPTSALDRDRQAAFLELLFSESSAAGATLIMV
                     SHDESLAPRFDSVLRLDEIAAIGQKDGA"
     gene            complement(4218..4817)
                     /locus_tag="VFR00_12340"
     CDS             complement(4218..4817)
                     /locus_tag="VFR00_12340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2796 domain-containing protein"
                     /protein_id="HEU0160102.1"
                     /translation="MRTTLRLVSALSLCLLLAAGLDLSHGRAQERQLGPHEHGRGVLN
                     LAIEGTRVSLELEAPGADIVGFEHAARTARQTAALKQAKQQLLAPQALFKFPSAAGCV
                     VAEARVEIEAGEHEHEDAKSPGAGGSKGEAAEDAHSNFHAEYAFNCKDPARITAIEFG
                     YFRTFAGAQKLEVNVITPKGQTKFDVTRDKARIDLAGMM"
     gene            complement(5111..>5594)
                     /locus_tag="VFR00_12345"
     CDS             complement(5111..>5594)
                     /locus_tag="VFR00_12345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009494033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU0160103.1"
                     /translation="DAALVLDISPAEVQSLIDEAGREIARQIATDVVIIEDEPLIALD
                     LERLVTSLGHRVVKIARTERQAIEAVSRTRPGLILADIQLADESSGLDAVNEILRQFS
                     VPVIFITAYPQRLLTGTRPEPTFLITKPFHPDNVKAIISQALFFDVRGRPEQPRLATA
                     "
BASE COUNT          981 a         1895 c         1828 g          890 t
ORIGIN      
        1 ctttcaggca gcgcagaagc gtcgatttcc cggtgccggc ccgacccgtg acgaaaaggt
       61 ggccgcttcc ctcgcgcaga tagtcatgcg cggcctgatg ctgtgcgctc gcctccaaac
      121 cacccggcca gatgcccttt cgcgtcgctg caattggctg ggatgtggcc accggtgccc
      181 ttcatttagg ccgcacgcgc acgggcgcgc actatacgcg atttgcgccg ccggcaagcc
      241 tagagcccgc ggcctccgct gacatccagg agcacaccgg taacgtagct tgccgcgtcc
      301 gagcacagcc acacgaccgc ctcggccact tcttgcgcgc tgcccccgcg cttcatcggc
      361 acgagatcct tgtactttgc cacgcggtcg ccgatgccaa agtccctctg gatgtcggtg
      421 tcgatcaagc ccggccgcac cgcgttgacg cggatgcctt cgcccgcaag ttccttggca
      481 agcccgatcg tgagggtatc gattgccccc ttcgagacgg catagtcggt cgattcgcca
      541 gccccgccca gcttggcggc gagcgaggac atattgacga tagcgccacc ctgcccgccc
      601 agccgcgttg acatgcggcg cacggcctcc tgcgccgcca ggaaggcccc ggtggtgttg
      661 actgccagga tctgctgcag ccgttccggc gcgacgtcga cgaacttggc tgcctggttc
      721 acaatcccgg cgttgttgaa aaaggcatcg aggcgcccga actcggcgtc gacggccttg
      781 aagagccgcg caatctcctc tggtttggcg atgtcggcct tcatggtcat ggccttgcgg
      841 ccggccttgc gcacgtcggc ggcaacggcc tcggccgcag cgccgcgcgt tgcatagttg
      901 attgccactg catagccgcg cgtggccgcc aagcgcgcgg tggccgcgcc gatgccgcgg
      961 ctggccccgg tgatgagcat caccttatct ggcattgctt gccccctcct tgttcagcat
     1021 cgctgcgggc gaggtcggcg cgctaccgtt cgccctcggc gcacgcccgc gcggcccggc
     1081 attgccgagg gaccgcgcca gggcagccga tcgtcccacc cttcaagaga acatcacgac
     1141 tggcaagcct caaacgcccg gccccctgaa ggcgcaaacc aggtattcgc cccccgagcg
     1201 cagcttgcgg atgcagacgt ggatgcgccc gtcgggcgac ctccgccaca gggcataatc
     1261 cggcgggatc accgcggaca agccgcgcga ggtcacaaaa tagctgccgt ccggccgctg
     1321 ctcgaccgtg tccgccggag cgcaatcttg gccgctgcag cattcgaggg gataccagtc
     1381 gtgggcgcgt gcggggttgc ccaaaaggcc cagggcggca atcaggatcg caatgcgatg
     1441 gtgtcccatg gctcgtctgc ccccaccatc gaccggccgg cgcacgcagc cccggccaac
     1501 gcgaggctcg gctgcgacca gcaagacccc gcccaacaac gcagtcggca cgcactagct
     1561 tggcaaaacg gccagcgcaa ggcgattgcc accacagcgt tgccggctca gtccttgagc
     1621 ggtcgcggtt cgaccttttc tgcctcgaga ctgtaaccag cctcggcaag acccgtgaag
     1681 gccggcgtga ccgtcagcgt ccccgtgacc cacacgggct cgaacgtgcc gcgcacgtcg
     1741 atgccgccct cggtcttcac gtaaacgatt tgattggggg gcgggggcgg cacgtggatg
     1801 caggcaccca cgaagggaac cagcaggaat tccttgaccc gtgtcgcgtc gaaatcgagg
     1861 gggaccacat agccaccgat ctgcacacgt ttgccgtcga ggcccgccac aacggccggt
     1921 gggctcgcgc tggacttggc gggggccgac atccagcgtc cctcgggcat cggctgcgtt
     1981 gtggacacgc catgctccgg acccgcgagc ccgctgggat cgccggggcg gccgctgaaa
     2041 aatgccttcg gcggcttggg cggggccggc ggcacgtgct gatcccattt gaggctcatg
     2101 atcgcctccc cggttcccgc cagcacgggc gcaaaaagcg cgcccgcgac gctcagcagg
     2161 ccaagcagga tggccacggg gaaggctcta ggcatgggac cctctcttca cgtcctgacc
     2221 gtcattccat ccgcaacgga caagcgatac gcgcgcagcg ccggcagcag gccagccaga
     2281 cagccggcgg cccagatggc ggcgagcgtc atgagctcgg ccggcttggg cgcatcgatc
     2341 ggcagatcga ggccgtaact gcggtcaagg tagggcgcga gcgccaccag gccgatgtaa
     2401 agaaggacga tgccgagcgc aattcccgtc gccgtgagca cgcccgcttc cgccatcagc
     2461 agcgccagaa tggtgcgtgc gcgggcgccg accgagcgca ggatggccat ctctcgacga
     2521 cgctcgttga gcgtcgtcaa aatcatggtg accatgccga gaagtgccgt cgccaccacc
     2581 atggccgaaa ccacagtcaa agccgtctcg gcgatgccga cgagccccca cagctcctgc
     2641 agggccgccc caggcagaat ggccgaaagc ggttcggcac gatactcgtt gatctcccgc
     2701 tgcaagcgaa acgtcgccag cttcgacttc agaccgacga gagcggcggt gatggccttg
     2761 ggcgtcagat ccatccggcg cacctcgtcg gctgatacgc tctgaccggg gatgcgcccg
     2821 ccgctttgcc aatcgacgtg aatggcttcg atcgcctcca gactgacatg caccgtccga
     2881 tcaaccggag tgccggtttt ttccaggatc cccgagacac ggaagggctt atcctcgtgc
     2941 tcgacgaagg ctaccgaacc gagcccgtgc gagatggtga tggggtcacc gagtttgtag
     3001 cccagcaccg cggcgacatc ggcgcccaga acggcatcga aaagatcgct gaacggtgct
     3061 cccgcgcgaa aggcaaggcc gtgcgtttgc cgaaagcggt agtggctgaa gtagtcgagc
     3121 gtcgtgccca acacgcgata gccatgatgg ccgtcgccca gagacagggg tacggcccag
     3181 gcgacttccg gccgggcccg aacatcctgg tagctttgcc aggataggtt gttggtggca
     3241 ttgccgatgc gaaacaccgc atagagcaaa aggttgaggc tgccactacg cgctcccacg
     3301 atcaaatcgg tgccggaaat cgtatcggcg aaactttggc gcgcaccggt gcggatcttt
     3361 tccacgccca gcagcagcat gacgctgacc gcaatcgcca gcaccgtcag caacgccgtg
     3421 agccagcgat tgcgcaaaga ctgcagcgcc agccgcaaaa cgatcatgcg ccgtccttct
     3481 gcccaatggc cgcgatttcg tcgagacgca gcacgctgtc gaagcgcggc gccaagctct
     3541 cgtcgtgact gaccatgatc agcgttgcac ctgccgcact gctctcgctg aagagcagtt
     3601 cgagaaaggc tgcttgccga tctcgatcca aggccgatgt cggctcatcg gccacgatca
     3661 gctccggtgc cccgatcagg gcgcgcgccg ccgccacgcg ttgctgttgg ccgacgctca
     3721 atttggccgc caacggcccg cggatgaggc ccgcctccag gccgagccga cccagcaacc
     3781 gcgcggcctc ctgttcgacc ggcgcccggg ccgcggcgcg gcggcgccgc ccgggcgcga
     3841 aactcagggg cagcaggaca ttgtcgagca cggtcccgta cggcaacagg ttgaacatct
     3901 ggaagatgat gccgaagtgc tcggcgcgga aagcatcccg cgcggacgcg gagaggcggg
     3961 tgatgtcgcg gcccaggatc tcgatcgatc cggcctgggg tgtcagcacg ccgcagagaa
     4021 ggccgaggaa cgtcgacttg ccgctgcccg acgggccgat caacagcagg cgcgcgccgg
     4081 cgggcaaggc gaagcggtcg atcgcgaggg caaagccgtt gcgccctggc caggtgaagc
     4141 gaacgtctga aacgcgcagc acgtgcgcgg cagcggcagg cgcttggcgg ctgagggcaa
     4201 ccatcgttca cccggaccta catcataccg gcaagatcga tgcgcgcctt gtcccgcgta
     4261 acgtcgaact tggtctgccc cttcggcgtg atcacattca cctccagctt ctgggcgccc
     4321 gcgaacgtcc ggaagtagcc aaattcgatg gccgtgatgc gggccgggtc cttgcagttg
     4381 aaggcatact cggcatggaa attggaatgg gcgtcctcgg ccgcctcgcc tttgctgcct
     4441 cccgcccctg ggctcttggc gtcctcgtgc tcatgctcgc cggcttcgat ctcgacacgc
     4501 gcctccgcaa cgacgcagcc ggccgcacta ggaaacttga agagggcctg cggcgcaagc
     4561 aactgttgct tggcctgctt cagcgccgcc gtctgacgcg ccgtgcgggc agcatgttcg
     4621 aagccgacga tatcggcgcc cggtgcctcc agctcgaggc tgacgcgcgt gccctcaatc
     4681 gccagattca aaacgccgcg gccatgctca tgtggcccga gctggcgctc ctgcgcgcgg
     4741 ccgtggctga gatcgaggcc cgcggccagg agcagacaga ggctcagggc cgaaaccaga
     4801 cgaagggttg tgcgcatgcc atgctcccca agtttcgctg gcggcctgcc gcgcccgccc
     4861 acgccatggc acgcccgggc tgccagccag gcggagacag acgggcagag cgccatcgag
     4921 ccacgcgtct gggatgttat aacgtagcaa taggcgtgcg cccagggggc tcgcgggaac
     4981 gtgacctggc ggcagcgagg gccgaccgtc cggggcgctg gccgtcaacc aacaaccttt
     5041 gtccgccggg tgtctcgcac accgggcgcc atgtcggccc ggcgcgcgtt ggtgtcgcta
     5101 cggccgtcgg ctaggccgtc gccagccgtg gctgttccgg ccggccgcgc acgtcgaaga
     5161 agagtgcctg gctgatgatc gctttgacgt tgtcggggtg gaacggcttg gtgatcagaa
     5221 atgtcggctc ggggcgcgtt cccgtcagca ggcgctgcgg ataggccgtg atgaaaatca
     5281 ccggtacgga gaactgacgc aggatttcgt tgaccgcgtc gagaccggag ctttcgtcgg
     5341 ccagttggat gtcggccagg atcaggccgg gccgggtgcg cgagaccgct tcgatggcct
     5401 ggcgctcggt gcgggcgatt ttgacgaccc ggtgccccaa ggaggtgacg agccgctcca
     5461 agtcgagggc aattaacggt tcatcctcga tgatgaccac atcggttgcg atctggcggg
     5521 cgatctcgcg gccggcctcg tcgatcaggc tctgtacctc cgccggagaa atatcgagca
     5581 cgagggcggc atct
//
LOCUS       DASTMT010000537         2947 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_3587373, whole genome shotgun sequence.
ACCESSION   DASTMT010000537 DASTMT010000000
VERSION     DASTMT010000537.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2947)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2947)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2947
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3587373"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..364)
                     /locus_tag="VFR00_12350"
     CDS             complement(<1..364)
                     /locus_tag="VFR00_12350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019199775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEU0160104.1"
                     /translation="MREQRGATRRGVVAGLTAAGLAAGAGLPVWAQSEPGQRFPLRPI
                     RLVVGFGPGGGNDILARLVAQKLSERLGQPVLVENKPGAGAIIATELVAKAAPDGYTL
                     LVGATGAMTINPAVYGKLP"
     gene            complement(441..1676)
                     /locus_tag="VFR00_12355"
     CDS             complement(441..1676)
                     /locus_tag="VFR00_12355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011503993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEU0160105.1"
                     /translation="MDFEDTPGEAEFRAGVRAFLGKAAKPRAPGTGMVYRAGNEDPAF
                     RAQAKAWQASKAEAGYAGITWPTHWGGRGGTPIQQVIYDQEEANYAVPRGLFDIGLGM
                     CIPTLCTWGTQEHRDRFAGKALRGEEIWCQLFSEPAAGSDLAGLRTRAEADGSDWVIN
                     GEKIWTSGAHYCDYGVLVTRSDFSARKHDGLTYFFLDMKSPGIEIRPIKQMSGASHFN
                     QVFFKDVRIPDSQRLGAVGQGWKVALTTLMNERYTIGGRMGAGVEDVFELARRLEIGD
                     GPALKHGAVRDKLADWYVASQGLKYANFRAMTALSRGETPGPEASLGKLVNASMLQTI
                     AAFAMDLMDEGGVLCDPELAPLAAAFQQTLLTSPASRIAGGSDEIMRNIIAERVLGLP
                     AEVRLDKTLPFNQLPTGKR"
     gene            complement(1689..2804)
                     /locus_tag="VFR00_12360"
     CDS             complement(1689..2804)
                     /locus_tag="VFR00_12360"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012109843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEU0160106.1"
                     /translation="MNFDFSDDLKALRDEARRFLRERCPVTAVRRILEGPEPYDLGLW
                     KEIAALGWTGAAIPETFGGAGLGHLGLCVLAEELGSALAPTPFSSSIYLAAEALMLAG
                     SPAQKETWLPKLASGEVIGTLAVAEGPAKVDPRKLRTSFRGSTLSGEKLPVPDGDVAD
                     LAVVAAQGERGPTLVLVDLKGRGVVRQPLETIDPSRSQARIVFDGAAAEVLGEAGAGE
                     ALLRRVEERAAVLFAFEQVGGAQRALEMARDYALQRYAFARPIGSFQAIKHKLADVYV
                     ANELARANAYYGAWALSSNAAELPVAAAAARISANEAYFLAAKENIQVHGGMGFTWEY
                     DCHLYYRRAKLLALALGSTRRWKERLLLELEAANAPL"
BASE COUNT          473 a         1039 c          953 g          482 t
ORIGIN      
        1 agggcagttt gccgtagacc gccgggttga tggtcatggc accggttgcg ccgacgagca
       61 gcgtgtagcc gtcgggggcg gccttggcca ccaattcggt ggcgataatg gcgccggcgc
      121 ccggcttgtt ttcgacgagc accggttgcc ccaagcgctc gctcagcttt tgcgctacga
      181 gccgcgccag gatgtcattg cccccgcccg gcccgaagcc gacgacgagc ctaatcggcc
      241 gtagagggaa gcgctgacct ggctcgcttt gcgcccaaac gggcagcccc gcgccggccg
      301 ccagacccgc cgccgtcagg ccggcgacca ctccgcgccg cgtcgcgccg cgttgctctc
      361 gcatggttca ccccctgtgc tctggcggcg cgtccgccca gctcggcgag gtcaagcgac
      421 cggtggcaag cccagggcgc tcaccgcttg ccggtcggca gctgattgaa cggcaaggtc
      481 ttgtcgaggc ggacctcggc aggcaagccc agcacgcgct cggcgatgat gttgcgcatg
      541 atctcgtcgg agccgccggc gatgcgcgag gcgggcgaag tcaatagcgt ctgctgaaag
      601 gcggcggcga gcggcgccag ctccgggtcg cagagaacgc caccctcatc catcagatcc
      661 atggcgaagg cggcaatcgt ctgcagcatc gaggcgttga ccaacttgcc aagcgaggcc
      721 tcgggtcccg gtgtttcgcc gcgcgaaagt gcggtcatgg cgcggaaatt cgcgtatttg
      781 agcccctggc tcgccacata ccaatcggca agcttgtccc gaaccgcccc atgcttcaag
      841 gctgggccgt cgccaatctc gagccggcgg gcaagttcga aaacgtcctc cacgccggcc
      901 cccatccgcc cgccgatcgt gtagcgctcg ttcatcagcg tcgtcagcgc cactttccag
      961 ccctggccca cggcgcccag gcgctggctg tcggggatgc gcacgtcctt gaagaacacc
     1021 tggttgaagt gcgatgcgcc cgacatctgc ttgatcggcc ggatctctat ccccggcgac
     1081 ttcatgtcga ggaagaagta ggtcaagccg tcgtgcttcc tggccgagaa atcgctgcgc
     1141 gtgaccagta cgccatagtc gcaatagtgg gcgcccgagg tccagatctt ctccccgttg
     1201 atgacccagt cgctgccgtc ggcctccgcg cgcgtgcgca ggccggcaag atcggagccg
     1261 gcggccggct cggaaaagag ctggcaccag atctcctccc cgcgcagggc cttgccggca
     1321 aagcggtcgc ggtgctcttg cgtgccccag gtgcacaggg tcggaatgca catgccaagg
     1381 ccgatatcga acaggccgcg cggcacggca tagtttgcct cttcctgatc gtagatcacc
     1441 tgctggatcg gcgtgccgcc gcgtccaccc caatgcgtgg gccaggtgat gccggcataa
     1501 ccggcctcgg ctttgctcgc ctgccaggcc ttggcctgcg cccgaaacgc gggatcctca
     1561 ttgccggcac ggtaaaccat gccggtgcct ggcgcgcgtg gcttggccgc tttgccgagg
     1621 aaggcgcgca cgccggcgcg aaactcggct tcccctggtg tgtcctcgaa atccatggct
     1681 ttctccgctc acaacggggc gttcgccgcc tcgagctcga gcagcaggcg ctctttccag
     1741 cgccgggtac tgccgagcgc cagagccaac agcttggccc ggcgatagta aaggtggcag
     1801 tcgtattccc aggtgaagcc catgccaccg tgcacctgaa tattctcctt tgccgccaga
     1861 aaataggctt cattggccga aatccgcgcg gcggcggccg ccacgggcag ctcggcggca
     1921 ttcgagctca gcgcccaggc gccatagtag gcgttggcgc gggcgagctc gtttgcaaca
     1981 tagacgtcgg cgagcttgtg tttgatcgcc tgaaacgagc cgatcggacg cgcaaaggcg
     2041 tagcgttgca gggcatagtc gcgcgccatc tccagggctc tttgcgcgcc gcccacctgc
     2101 tcgaaggcaa ataacaccgc cgcgcgttcc tcgacccggc gcagcaacgc ctcccccgcg
     2161 cccgcctcgc ccaggacttc tgcggccgcg ccgtcgaaca cgatgcgtgc ctgcgaccgg
     2221 ctgggatcga tggtttcgag cggctggcgc acgacgccgc gccccttgag gtcgaccagc
     2281 accagcgtgg gcccgcgttc gccctgggcg gccaccaccg ccaggtcggc gacgtcgccg
     2341 tccgggacag ggagtttctc gccgctgagc gtgctgccgc gaaagctcgt gcgcagcttg
     2401 cgcggatcga ccttggcggg tccttcggcc accgccagcg tcccaatgac ctcgccgctg
     2461 gcgagtttcg gcagccaagt ctccttctgc gcggggctgc cggccagcat gagggcttcc
     2521 gccgccagat agatcgagga ggaaaacggc gtcggcgcca gcgccgagcc cagttcctcg
     2581 gcgagcacgc acaagccgag atggccgagc ccggcgccgc caaacgtctc cggaatggcc
     2641 gcgccggtcc aacccagggc ggcgatctcc ttccagaggc cgaggtcgta gggctctggc
     2701 ccctcgagaa tgcgccgcac cgccgttacc ggacagcgct cgcgcagaaa tcgacgcgcc
     2761 tcatcgcgca gcgccttgag gtcgtcgctg aaatcgaaat tcatcgctgc ccgctcccca
     2821 ttggctagct cggcaggcga cccttcacct cgcgcaagga gccgccggcc tcggccagct
     2881 cttgcagcaa agcggagggt ttccagcgcg ccccatagcg ctgatgccag accgccacct
     2941 ggttgta
//
LOCUS       DASTMT010000538         4296 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_641664, whole genome shotgun sequence.
ACCESSION   DASTMT010000538 DASTMT010000000
VERSION     DASTMT010000538.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4296)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4296)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4296
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_641664"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..735)
                     /locus_tag="VFR00_12365"
     CDS             complement(<1..735)
                     /locus_tag="VFR00_12365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013419336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0160107.1"
                     /translation="MQQQTIICMKWGTRYGVDYVNRLASMIRRHTRRPTRIICFTEQP
                     HGIEAGVETAPLPPINIPERVQWLGWRKISLWQAPLLDLAGDVLFLDLDVVITGSLDE
                     FFDFEPGRYCVARNWSEPHLRVGNTSVYRFPVGRMTYIFDEFHRDGERFLARYRNSQK
                     YISGEAKDMAFWPPEWCLSFKHSLLPRWPLNFFVVPKLPAGTKVVAFTGKPDPDEARD
                     GHWPVKSPWKRLYKHVRATPWIAEHWR"
     gene            complement(814..2022)
                     /locus_tag="VFR00_12370"
     CDS             complement(814..2022)
                     /locus_tag="VFR00_12370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020083992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEU0160108.1"
                     /translation="MSDARPTIAQIVPNLDAGGAEVTSVEVTEAIVKAGGRALVVSEG
                     GRLAARIVAAGGELERLPVASKNPARILANVRRLKALARREGVSLLHARSRAPAWSAL
                     LAARSLGLPLVTTYHGAYRERSRLKNLYNGVMARGDVVIANSGYTARSIKGRYGKPDD
                     GIAVIYRGVDLERFAPEAVAPERIAELRQRWGVLASQRVILHAARLTPLKGQGVVIAA
                     ARRLATAGAGDDLVFVLAGDAGPRAGYAAGLKAEIRAAGLEGRVRLVGHLEDMPAGLA
                     AADVALMVSTEPEGFGRVAAEAQAMGRPVIVSEIGALPEAVRAASAVGAAAATGWLVP
                     PGDAEALAGAILNALALPAAARQAMGERARAFVMANYSVRELQRLTLAVYDRLLGTRL
                     ADRFGAGPTP"
     gene            2278..3612
                     /locus_tag="VFR00_12375"
     CDS             2278..3612
                     /locus_tag="VFR00_12375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020178139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HEU0160109.1"
                     /translation="MDPQAELRAKIAAGTAVVGIYGLGYVGLPLALAIHARSRYRVIG
                     FDIDQQKIDSLNEGRSYFRHLPSDSIARMCASGRFAATSEFQRTREVDVALMCVPTPL
                     NRYREPDLSYVTNTARSILEYVRPGQLLVLESTTYPGTTRDLLMPIYRQRGLALGRDV
                     FLAYSPEREDPGNAEFETSRIPKVVGGDGATARDLAVSFYSAFVDKVIPVSSTACAEA
                     VKLTENIFRAVNIALVNELKLIYEAMGIDIWEVIEAAKSKPFGYMPFYPGPGLGGHCI
                     PIDPFYLTWKAREYGISTRFIELAGEINTVMPRVVVERLAEALDRRLGRPLSGSRVLI
                     IGLAYKRNIDDLRESPSLKLIELLQARGATADYHDPYIASIPNTREHPELAGRTSIPL
                     ASADLAAYDAVLIATDHDGIDYRALVAGAKLVVDTRNVCERSGIAADNVVKA"
     gene            3619..>4296
                     /locus_tag="VFR00_12380"
     CDS             3619..>4296
                     /locus_tag="VFR00_12380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEU0160110.1"
                     /translation="MGPADDIVSFLRVGSGAAERRIAYLSGAGRTPGVLWLSGFCSEM
                     TSTKASALATWAKQQGLASLRFDYSGHGRSGGQLQEGSISRWLEEAEAVFTQLSRGPQ
                     VLVGSSMGGYIALLLLRNMLARAASEAERIRALVLIAPAWDMTELMWNNLPASARQTI
                     SSTGVYLRPSRYGDGPYPITRSLIEDGRRHLIADAPFDPGRPLHILHGLQDPDVPWEH
                     TLDLVAHL"
BASE COUNT          735 a         1474 c         1366 g          721 t
ORIGIN      
        1 acgccaatgc tcggcgatcc agggtgtggc ccgaacgtgc ttgtacaacc gcttccaagg
       61 gctttttacc ggccaatgcc cgtcgcgcgc ttcatcgggg tcgggtttgc cggtgaaggc
      121 caccaccttg gtccccgccg gcaacttcgg caccacgaag aaattgaggg gccagcgcgg
      181 cagcagcgaa tgcttgaagc tgaggcacca ttcgggcggc caaaacgcca tgtccttggc
      241 ttccccggaa atatatttct gcgagttgcg gtaacgcgcc agaaagcgtt ctccgtcccg
      301 gtgaaactcg tcgaaaatgt aggtcatgcg ccccaccggg aagcggtaaa ccgaggtatt
      361 gccgacgcgg aggtgtggtt ccgaccagtt gcgggcgacg cagtagcgcc ccggctcgaa
      421 atcgaaaaac tcgtcgagcg agccggtgat gaccacgtcg agatctaaaa acaagacatc
      481 gccggccaga tcgagcaggg gagcctgcca cagcgagatc ttgcgccagc ccagccactg
      541 cacgcgctcg ggaatattga taggcggcag cggcgcggtc tcgacgcccg cctcgatgcc
      601 gtgcggttgc tcggtaaagc agatgatgcg cgtcggccgg cgcgtgtggc ggcggatcat
      661 ggaggccagg cgattgacat agtcaacgcc atagcgcgtg ccccatttca tgcagatgat
      721 ggtttgttgt tgcatcggct tgaccgctgg ctgagctccg ggcgcgcgcc cctcatgact
      781 gcgggcggga atggaggagg gatggtcgcc ctgtcaaggg gtgggaccgg cgccgaagcg
      841 gtcggccaag cgcgtgccga gcagacgatc gtagaccgcc agcgtcaggc gctgcagctc
      901 gcgcacggaa taatttgcca tcacgaacgc gcgcgcccgc tcccccatgg cctggcgtgc
      961 cgccgccggg agcgccaggg cgttgagaat cgcacccgcg agcgcctcgg catcccccgg
     1021 gggtaccagc catccggtgg ccgcggccgc gccgaccgcg gaggcggcac gcaccgcctc
     1081 cggcagcgcg ccgatctccg agacaatgac gggccggccc atggcttgcg cctccgccgc
     1141 aacgcggccg aagccttcgg gttcggtcga taccatcaaa gccacgtcgg ccgccgccaa
     1201 acccgccggc atatcctcga ggtgacccac caggcgcacc cggccctcca gcccggccgc
     1261 gcggatctca gccttcagac cggccgcata gcccgcgcgc ggtcccgcat cgccggcgag
     1321 cacaaagaca agatcgtcgc cagcccccgc tgttgccagc cgccgggccg ccgcgatgac
     1381 aactccctgg cccttgagcg gagtgagacg cgccgcatgc aggatgacac gttgcgaggc
     1441 cagcaccccc cagcgctgcc gcagctcggc aatgcgctcg ggtgccaccg cctcaggtgc
     1501 gaagcgctcg agatcgacgc cacgataaat gacggcaatg ccgtcgtcgg gcttgccgta
     1561 gcgacctttg atcgagcggg ccgtatagcc cgagttggca atgacgacgt cgccgcgcgc
     1621 catcacgccg ttgtagaggt tcttcagtcg gctgcgttcc cgataggccc catggtaggt
     1681 cgtcaccagc ggcaagccga ggctgcgcgc ggccaacagc gcgctccagg ccggcgctcg
     1741 actgcgggcg tgcagcaggc tcacgccctc gcgccgggcc agcgccttga gacggcgcac
     1801 attcgccaag atgcgcgcgg gatttttgct ggcgaccggc agccgttcga gctcgccgcc
     1861 agccgccacg atgcgcgcgg ccaacctgcc gccttcgctg acgaccaggg cgcggccccc
     1921 ggctttgacg attgcctccg tcacctccac gctggtgacc tcggcgccac ccgcgtcgag
     1981 gttgggaaca atttgggcaa tcgtgggccg cgcgtcagac aaggaggaag ctctcctgca
     2041 agatgaaagg ctcgctggtt tcaagcacgg gcctccccgc cctgcaagcg agaggcggcg
     2101 ctgcccccag cgttgccgcc gatggacaag ccgtcccctt caatgccata ggaggcccgc
     2161 gcctccggcc gatcgcaccc tccggcgtgc cgtgcgggag gcgcgcgcct cgcgcggcga
     2221 tgcgcaggcg ccaagcggtc gcatcttccc gattggaccc acacgcacgg ctttttgatg
     2281 gacccacagg cagagcttcg cgccaaaatc gccgccggca ccgccgttgt cggcatctat
     2341 gggctgggct acgtcggttt gcccctcgca ctggcaattc atgcgcgcag ccgctaccgc
     2401 gtgatcggct ttgatatcga tcagcagaag atcgacagcc tgaatgaggg ccgctcctat
     2461 tttcgacatc tgccgtccga cagcatcgcc cgcatgtgcg cctcgggacg ctttgcggcc
     2521 accagcgagt ttcaacgcac gcgcgaggtc gatgtcgcgc tcatgtgcgt gccgaccccg
     2581 ctcaatcgct accgcgaacc cgatctctcc tatgtgacca acacggcgcg ctcgatcttg
     2641 gagtacgtcc gtccgggtca gctcctggtg ctggaatcca ccacctatcc cggcaccacg
     2701 cgggatttgt tgatgccgat ctatcgacag cgcgggctcg cactaggccg ggatgtgttt
     2761 ttagcctatt cgcccgagcg cgaagatccg ggcaatgccg agttcgagac ctccaggatc
     2821 cccaaggtcg tcgggggcga cggcgcgacg gcgcgcgatc tggcggtcag cttctatagc
     2881 gcctttgtcg acaaggtcat cccggtgtcg tcgaccgcct gcgccgaggc ggtgaaactg
     2941 acggagaata ttttccgtgc tgtcaatatt gccctcgtca acgagctcaa gctcatctat
     3001 gaggcgatgg gcatcgatat ctgggaggtg atcgaggctg ccaaatcgaa gccgtttggc
     3061 tatatgccgt tctaccctgg tccaggactc ggcggccatt gcatcccgat cgacccgttt
     3121 tatctgacct ggaaggcgcg cgagtatggt atctcaacgc gcttcatcga gctcgccggg
     3181 gaaatcaaca cggtcatgcc gcgcgtcgtc gtcgaacgcc tggccgaagc gctcgatcgc
     3241 cgcctcggcc ggccgctctc gggcagccgc gtcctgatca ttggcctcgc ctataagcgc
     3301 aacatcgatg atctgcgcga aagcccttcg ctcaagctga tcgaactttt gcaggcccgc
     3361 ggcgccacgg ccgactatca cgacccctac atcgccagca tcccgaacac gcgcgaacac
     3421 cctgagctgg ccggtcgcac ctccattccc ctcgccagcg ccgacctcgc tgcctacgac
     3481 gcggtcttga ttgccaccga tcacgacggc atcgactacc gggcactggt ggcaggcgcc
     3541 aagcttgtgg tcgatacgcg caatgtctgc gagcgcagcg ggattgcggc cgataacgtc
     3601 gtgaaagcct gaggcgccat ggggccggcg gacgatatcg tttcgttttt gcgggtgggc
     3661 agcggggccg ccgaacgccg cattgcctac ctcagcggcg ccgggcgcac cccgggcgtg
     3721 ctctggctga gcggcttttg ctcggagatg accagcacca aagcctcggc gctggccact
     3781 tgggccaaac aacaggggct tgcctcgctg cgcttcgact attcgggcca cggacgctcc
     3841 ggcgggcagt tgcaggaggg cagcatcagc cgctggctcg aggaggccga ggcggttttc
     3901 acacagctga gccgcggccc ccaggtgctg gtcggctcca gcatgggcgg ctacatcgcc
     3961 cttttgctgc tgcgcaatat gctggcgcgg gccgccagcg aagcggagcg cattcgtgcc
     4021 ttggtgctga tcgcaccggc ctgggacatg accgagttga tgtggaacaa tctgccggcg
     4081 agcgcgcggc agaccatctc atcgaccggc gtctacctgc gaccctcgcg ctacggcgac
     4141 ggcccctatc ccatcacccg gagcctgatc gaggatggcc gccggcattt gatcgccgat
     4201 gcacctttcg acccggggcg cccgctgcac attctgcatg gactgcagga ccccgacgtg
     4261 ccctgggagc acacgctcga tctcgtcgcc cacctc
//
LOCUS       DASTMT010000539         2326 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_2932418, whole genome shotgun sequence.
ACCESSION   DASTMT010000539 DASTMT010000000
VERSION     DASTMT010000539.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2326)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2326)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2326
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2932418"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(220..840)
                     /locus_tag="VFR00_12385"
     CDS             complement(220..840)
                     /locus_tag="VFR00_12385"
                     /inference="COORDINATES: protein motif:HMM:NF017849.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF934 domain-containing protein"
                     /protein_id="HEU0160111.1"
                     /translation="MPVLEADPGAPVSEAAAANLAGCLPGGIGRPGLSGPADPNGPIW
                     KEGAFRLDPFQVAPASGELGAGPLLVAKARWLGDRAQLLARGGPLGLFLEAGMSLDDV
                     ADDVGRFVLIALNFPKFSDGRAFSTARLLREKFGYRGELRAVGNVLCDQIPFMRRVGF
                     DAFEVSHAPTRRALGAGELAEVRLYYQPVGAAAEPGTRSWLRTGAA"
     gene            complement(827..>2326)
                     /locus_tag="VFR00_12390"
     CDS             complement(827..>2326)
                     /locus_tag="VFR00_12390"
                     /EC_number="1.8.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018181091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050311 - sulfite reductase (ferredoxin)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HEU0160112.1"
                     /translation="QLHAYMLRIAIPYGTLSTRQLRKLAYLARTYDRGFGHFTTRQNL
                     QFNWPKLSDIPDILDELAEVEMHAIQTSGNNVRNVTADHFAGATAEEVVDPRPYAEII
                     RQWSSLHPEFSFLPRKFKIGITASERDRAAIQTHDIGLQLKRLADGGLGFAVWVGGGQ
                     GRTPYLAKKIRDQLPEADILSYIEAILRVYNLHGRRDNIHKARIKILINSIGADVFAA
                     EVEAEWARIKDGVLKLPQAEIERIAAYFAAPAFAARPPPAGLRPADRGFDRWVTRNTH
                     AHKQPGHAIVTITLKPIGGIPGDASAAQMEAIADLAERFSFAEVRVSHEQNLVLPHVR
                     LGDLWAVYQELKRIGLAEANAGLVTDIIACPGLDYCALANARSIPIAQRISARLAPRQ
                     EQVGELKIKISGCINACGHHHVGAIGILGVEKRGEEHYQLLLGGSGAEDAALASITGP
                     GFGPDGIVDAVETVVDTYLKLRTGPQEAFLAAYARLGAQPFKEALYARA"
BASE COUNT          391 a          743 c          824 g          368 t
ORIGIN      
        1 atagtcggag gcgagatagg gcggatcggc gacgttgccg gcacgggcat aacgatcgtg
       61 catggctggc gagggctcct ctgcagcgcg gcgggcagca ggcgcgcggc agcgcaccag
      121 ggctgacctt gtgattgctc gccccgaaaa gcaagtgcgc aatcccgccc agccgcgcgc
      181 ttacgcaagc ggcggcgcgt ggcgttgcct cggtggcggt taggcggcgc cggtccgcag
      241 ccagctgcgc gtgcccggct ctgccgccgc gccgaccggc tgataataaa ggcgcacctc
      301 ggccagctcg cccgcgccga gcgcgcgtcg ggtcggcgca tggctgacct cgaaggcatc
      361 aaacccaacc cggcgcatga aggggatctg gtcgcagagc acgttgccga cggcacgcag
      421 ctcgccgcga tagccgaact tctcgcgcag gagccgcgcc gtggagaagg cccgcccatc
      481 ggaaaatttc gggaaattga gggcaatcag gacgaaacgg ccgacgtcat cggcgacatc
      541 gtcgaggctc atgccagcct ccaggaaaag gccgagcggt ccgccccgcg ccaacagctg
      601 cgcgcggtcg ccgagccagc gcgccttggc aacgagcagc ggcccggcgc cgagctcgcc
      661 gctggccggc gcgacctgaa aggggtcgag ccgaaaggca ccctccttcc agatcggacc
      721 gttgggatcg gccgggccgc tcaaacccgg ccggccgatg ccgccgggca ggcaacccgc
      781 caggttcgcc gcggcggcct cacttaccgg cgcgcctggg tcggcctcaa gcacgggcat
      841 aaagggcctc tttgaagggt tgggcgccga ggcgggcgta ggcggcaaga aaggcctcct
      901 gcggccccgt gcggagtttc agataggtgt cgaccaccgt ctcgacggca tcgacgatgc
      961 catcggggcc aaagcccgga ccggtaatgc tggccaaggc cgcatcctcc gcgcccgaac
     1021 cgcccagcag cagctggtag tgctcctcgc cccgcttctc gacgccgaga atgccgatcg
     1081 ccccgacgtg atggtgtcca caggcgttga tgcagccgga aatcttgatc ttgagctcgc
     1141 ccacctgctc ctggcgcggc gccagccggg ccgagatgcg ttgcgcaatc ggaatcgagc
     1201 gcgcattggc gagcgcacag tagtcgaggc ctgggcaggc gatgatgtcg gtcaccagcc
     1261 cggcgttggc ctcggccaga ccgatccgct tcagctcttg atagaccgcc cagaggtcgc
     1321 ccagccgtac gtgcggcagc accaggtttt gctcgtggct gacgcgcact tcggcgaagg
     1381 agaagcgttc cgccaggtcg gcaatggcct ccatttgcgc ggccgaggcg tcgccgggga
     1441 tgccgccgat cggtttcaag gtgatggtga caatggcgtg gcccggctgc ttgtgggcgt
     1501 gggtgttgcg cgtcacccag cgatcgaagc cgcgatcggc cgggcgcagg cctgcgggcg
     1561 gcggccgcgc cgcaaacgcg ggcgccgcga aataagccgc gatccgctca atttccgcct
     1621 gcggcagctt gagcacgccg tctttgatgc gggcccattc cgcctccacc tcggcggcaa
     1681 agacgtcggc gccgatgctg ttgatgagaa tcttgatgcg cgctttgtgg atgttgtcgc
     1741 gccggccgtg caggttatag acgcgcagaa tggcctcgat gtagctgagg atgtccgcct
     1801 cgggcagttg atcgcgaatc ttcttggcca ggtagggggt gcggccctgg ccgcccccga
     1861 cccagaccgc aaaacccaag ccgccgtcgg cgaggcgctt gagctggaga ccgatgtcgt
     1921 gggtctgaat ggcggcgcgg tcacgctccg aggcggtgat gccgatcttg aacttgcgcg
     1981 gcaaaaagct gaactcgggg tgcagggagg accattggcg gatgatctcc gcatagggtc
     2041 gcggatcgac gacttcttcg gccgtggcac cggcgaagtg atcggcggtg acgttgcgca
     2101 cgttgttgcc cgaggtctgg atggcgtgca tttccacctc ggccagctcg tcgagaatgt
     2161 cgggaatgtc ggacagcttg ggccaattga attgcaggtt ttggcgcgtc gtgaagtgac
     2221 cgaagccccg atcgtaggtg cgcgcaaggt aggcgagctt gcgcagctgg cgagtactca
     2281 acgtgccgta gggaatggcg atgcgcagca tataggcgtg cagctg
//
LOCUS       DASTMT010000540         1514 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_3762835, whole genome shotgun sequence.
ACCESSION   DASTMT010000540 DASTMT010000000
VERSION     DASTMT010000540.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 1514)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1514)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1514
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3762835"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..950)
                     /locus_tag="VFR00_12395"
     CDS             complement(<1..950)
                     /locus_tag="VFR00_12395"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEU0160113.1"
                     /translation="MPLARRSLLKALAAGSAAAWLGRPSWGQATGPTQASEIDPDKAK
                     AEGKVVLYTSLDTQIVDAINAAFKQKYGIDVQYFRGGSSDVTSKVLAEADAGRPQADM
                     VDASDLAALLLMKERGLLKPFASAAMPAVAKELRDGDATWITDRLTQALIQFNTKEFG
                     AQPPASWADLGNSAMQGRLVYFSSANGDGAPRIYTLARHLGWDVVKAMAATKPLRVQT
                     PQVITQVLERGERGVGFLQNDNIAWRSKLQGKSTDYVFPGEGVPSEIGACGLLRSAQR
                     PHAAALFHEWWMGTEGQAILVKGGKYSSRLDVPPPEGSTPL"
     gene            1169..>1514
                     /locus_tag="VFR00_12400"
     CDS             1169..>1514
                     /locus_tag="VFR00_12400"
                     /inference="COORDINATES: protein motif:HMM:NF012354.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEU0160114.1"
                     /translation="MDYRQLQLFLATAERLNLSHAAEAMNITQPGLSKSMHRLQKELG
                     TRLYHRRGRGIELTESGRALLRHVKVIETQLSDARSEVMGIAGGTLGHARIGAGPSWL
                     SRHLPETIARVMQ"
BASE COUNT          249 a          505 c          504 g          256 t
ORIGIN      
        1 agcggcgtgc tgccctcggg tgggggcaca tcgaggcggc tcgaatattt gccgcccttg
       61 acaagaatgg cttgtccctc cgtccccatc caccactcgt ggaagagcgc ggccgcgtgt
      121 ggccgctgcg cactgcgcaa caagccgcac gccccgatct cgctcggcac accttcgccg
      181 ggaaagacgt agtccgtcga cttgccctgc aatttggagc gccaggcgat gttgtcgttt
      241 tgcaaaaagc cgacaccgcg ttcgccgcgc tccagaacct gggtgatgac ctgcggcgtc
      301 tgcacgcgca aaggcttggt ggcggccatc gccttgacca cgtcccagcc caagtgccgg
      361 gcgagcgtgt agatacgcgg tgcaccatcg ccgttggcgc tggaaaagta gacgagccgg
      421 ccctgcatgg cgctgttgcc cagatcggcc cagctggccg ggggctgagc gccgaactcc
      481 ttggtgttga actggatcag cgcctgcgtc agccggtcgg tgatccaggt tgcatcgccg
      541 tcgcgcagct ctttggcgac ggccggcatg gccgccgagg cgaagggttt gagcagcccg
      601 cgctccttca tgagcaggag ggcggccaaa tccgatgcat cgaccatatc ggcctgtggt
      661 cgaccggcgt cggcctcggc caggaccttc gaggtgacgt cgctcgaacc gccgcggaag
      721 tactgcacat cgatgccgta cttctgcttg aaggcggcat tgatggcatc gacgatttgg
      781 gtgtcgaggc tggtatagag gaccaccttg ccctccgcct tagccttgtc ggggtcaatc
      841 tcgcttgcct gtgtcgggcc ggtagcctgg ccccagctgg gacggccgag ccaagcggcg
      901 gccgagcctg ccgccaaggc cttgagcagg gatcgtcggg cgagaggcat ggcggtctcc
      961 ctatggtgaa cgcgggcccc acagtctggg cccaaagttt tgccagcggc gccgcctggc
     1021 gccgacggct tacggctcag gtgcgcgcga gcgcggcgcc tcacgtcccc cgcgcgcgcc
     1081 gccgctcgtt gccgcaaaaa cgtgcaggcc ttgccggctg cgggcaattg acggagggta
     1141 gcacgaacca tgactacaga gacatagtat ggactaccgc cagctgcagc tctttctcgc
     1201 caccgccgaa cgcctcaatc tctcgcacgc ggccgaggcg atgaatatca cgcaaccggg
     1261 gctctccaaa agcatgcacc ggctgcagaa ggaactgggc acccggctct atcaccggcg
     1321 cgggcgcggc atcgagctga ccgaatctgg gcgcgccctg ctgcgccacg tcaaggtgat
     1381 cgagacgcag ctgtccgacg cgcgttccga ggtgatgggc attgccggcg gcacgctcgg
     1441 gcacgcgcgc atcggcgccg gtccgtcttg gctgagccgt cacctgcccg aaacgatcgc
     1501 ccgggtgatg cagc
//
LOCUS       DASTMT010000541         4457 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_4751495, whole genome shotgun sequence.
ACCESSION   DASTMT010000541 DASTMT010000000
VERSION     DASTMT010000541.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4457)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4457)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4457
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4751495"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(234..752)
                     /locus_tag="VFR00_12405"
     CDS             complement(234..752)
                     /locus_tag="VFR00_12405"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HEU0160115.1"
                     /translation="MFTRSSAAEPKPLEPMNTTPNLNVQRRPSPPDPSTKSVISNDLK
                     IIGQGLKIISQGTLQVDGEVEGDVRGTEVIIGEKGKVTGTVAAERVIVRGTICGVIRG
                     MTVTLETSSRVEGDIHHMSLAIEQGAEFDGRCKRPADASELNLELDGSSVAAAPQPRP
                     VVHRAGQQVRPG"
     gene            991..1908
                     /locus_tag="VFR00_12410"
     CDS             991..1908
                     /locus_tag="VFR00_12410"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011503345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEU0160116.1"
                     /translation="MLSARKIAAAIVLSAIGALPAATQAAEPCQNANALGVARTVEID
                     TTGGPGFGTEQYKMHDFLLLKEVVLTFDDGPWPEHTRAVLAALAHHCTKAIFFPIGKH
                     ALWHPEILKEVAAAGHTIGSHTWSHANLEKVKGEKATEEIEKGLSAVSLALGAPPAPF
                     FRFPFLKDPKDQLAYLGTRNVAIFSHDLDSFDFKMRKPEDVIKSVLAKLDKKGKGIIL
                     MHDFQGATAKAMPELLEQLKAKGYKVVHAKAKTAVTTLARWDEAVKGEVKGAAVGPDR
                     PTSSVVRTIDEAAPAVMAKPAASPAAPSK"
     gene            1975..2526
                     /locus_tag="VFR00_12415"
     CDS             1975..2526
                     /locus_tag="VFR00_12415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007752710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF924 family protein"
                     /protein_id="HEU0160117.1"
                     /translation="MPGSTWAEEVGRFWFEETAPEQWFRKDEAFDTLLRQRFAHTHEH
                     VAALAPDACLGDPQTALAAIIVLDQFSRNMFRGSPRAFASDAAALGLAEAAIAKGFDG
                     EVAERARLFFYLPFEHAEDSAAQARGVALVARLADPELVKWAEAHQAIIDRFGRFPHR
                     NAVLGRLSTAQERDFLAQPGSSF"
     gene            complement(2540..2845)
                     /locus_tag="VFR00_12420"
     CDS             complement(2540..2845)
                     /locus_tag="VFR00_12420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0160118.1"
                     /translation="MVTRRLLASSLSVGLLAGVGVFAPGGGHAQAPPADLSPPTAEAP
                     AAAPPGDSRVTTPYADVTVNADRGKVRVEGPYGSVRVDPDAGQVKVRVPYVNLDVRW"
     gene            complement(2966..3475)
                     /locus_tag="VFR00_12425"
     CDS             complement(2966..3475)
                     /locus_tag="VFR00_12425"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003600767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEU0160119.1"
                     /translation="MDATALKALQAPLKDRYREAPETAVITLKAAGHLGEGVTCSVQT
                     GKALVEAGLHPATGGTGMHACSGDMLLEALVACAGVTLNAVATALQLTVRDARIRAEG
                     DLDFRGTLGVAKEAPVGFRDIRLSFEIDGDLSEEQKATLMKLTERYCVVYQTLRQPPP
                     IALDSRMTS"
     gene            3666..>4457
                     /locus_tag="VFR00_12430"
     CDS             3666..>4457
                     /locus_tag="VFR00_12430"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEU0160120.1"
                     /translation="MPASVARAPAATSLAIEGGRFLLVGGASLVGSASAQRFLDAGAA
                     EVVVFDNLSQGSLAALHHLEGNPRLKIVAGDVLRLPQLLAAMRHVDGVLHLAALMSLS
                     MDRDPWTGLDVNIRGTQNVIEACCVIGVKKLVLASSSAAYGYGPGVVGKLIETSPFHS
                     SGAPPGAILYGASKIIGEQLCRDVHRKRGLNYVVVRYATVYGERQHYRAANALYIIDT
                     HDRVRRGERPRVLGDGSEVKHFVHVSDVARANAAAFASDATDVAVN"
BASE COUNT          752 a         1453 c         1481 g          771 t
ORIGIN      
        1 acggtcggcg ggcagtagcc gctgccgagc cgcaagcgac cagcttaggc tctggtggag
       61 gctgctccaa cgcaggcgtg atcgagcctc agcgcactcg ccgatatcaa gatcccactc
      121 gacattggat cacctccttt ccgatggttg cgattcttgc caactgtagc acgcgcgccg
      181 cagtttggaa gggggtaaac agggggccgc gcgccggccc cgcgcgcttc cctttagccc
      241 ggccgaactt gctggccggc gcggtggacc accgggcggg gctgcggcgc ggcggcgaca
      301 gaggagccgt caagctcgag gttcaactcg gaggcatcgg cggggcgctt gcagcggccg
      361 tcgaattcgg cgccctgctc gatggcgagc gacatatggt ggatgtcgcc ctccacgcgc
      421 gaggaggttt ccagcgtcac cgtcatgccg cgaatgacgc cgcagatcgt gccgcgcacg
      481 ataacgcgtt cggcggcgac cgtgccggtg accttgcctt tctcgccgat aatcacttcg
      541 gtgccgcgca cgtcgccttc cacctcaccg tccacctgca acgtaccctg gctgataatc
      601 ttgaggccct gaccaatgat tttcagatcg ttgctgatca cggacttggt cgaggggtcc
      661 ggcggcgacg ggcgccgttg cacgttgagg ttaggtgtgg tgttcatcgg ctcgagtggt
      721 ttcggctcgg ctgccgagct acgcgtaaac atgaaagccc ccgggtgatg ccacgaataa
      781 acagctagcg cgcgatcaca acaccgccag atggtcgaat tatgtccgcg cggcaacagc
      841 gtgcaaagtc cggcggcgga tggcaagacc tatcgagcgc tgcgggcgca aatattgcgg
      901 tgcgaacgcc gggacttgcg cgtataccaa agatgatctg tggttaacca aacgcagctt
      961 accggaaacc caattccccg ggggattgtc atgctctcag ccagaaagat tgcggccgca
     1021 atcgtcctgt ccgcaatcgg tgcgcttccg gccgccacgc aggcggcgga gccttgccaa
     1081 aacgccaacg cgctcggcgt cgcgcgcacc gtggaaatcg acaccaccgg cggtccaggc
     1141 ttcggcaccg agcaatacaa aatgcacgac ttcctgctgc tcaaggaagt ggtgctcacc
     1201 ttcgacgatg gaccatggcc cgagcacacc cgcgcggtgt tggccgccct ggcgcaccac
     1261 tgcaccaagg cgatcttctt tccgatcggc aagcatgcgc tgtggcatcc ggagatcttg
     1321 aaggaagtgg ccgcggcggg gcacacgatc ggctcgcaca cctggtcgca cgccaatctc
     1381 gagaaggtga agggtgagaa ggcgaccgaa gaaatcgaaa aaggtctgag tgcggtgagc
     1441 ttggcgctgg gcgcgccgcc cgccccgttc ttccgttttc ccttcctgaa agacccgaag
     1501 gaccagctcg cctatctcgg cactcgcaac gttgccatct tctcgcacga tctcgacagc
     1561 ttcgacttca agatgcgcaa gcccgaagac gtcatcaaat cggtgctggc caagctcgat
     1621 aagaagggca aaggcatcat tctgatgcac gattttcagg gcgcgaccgc caaggccatg
     1681 ccggagctgc tggagcagct caaggcgaaa ggctacaagg tcgtgcacgc caaggccaaa
     1741 accgcggtga cgacgctggc gcgttgggac gaagcggtga agggcgaagt gaagggcgcc
     1801 gccgtgggtc cggatcggcc gacctcgagt gtggtgcgca caatcgacga ggcggcgccg
     1861 gcagtgatgg ccaaacccgc cgcgtcgccg gcagctccca gcaaatagcg cggccgggct
     1921 cgaaggtctc gcggcgctgg cgcacgcgcc gatcgtgcga caacggagcg cgtcatgcca
     1981 ggcagcacgt gggccgagga ggtggggcgc ttttggttcg aggagactgc gcccgaacaa
     2041 tggttccgca aagatgaggc cttcgacacg cttctacgcc agcgctttgc gcacacgcac
     2101 gagcacgttg ccgccctcgc gcccgatgcc tgtttgggcg atccccagac ggcactcgcc
     2161 gccatcatcg tgctcgatca attttcgcgc aacatgttcc gtggcagccc gcgcgcgttt
     2221 gccagcgacg ccgcggctct aggcctcgcc gaagccgcga ttgccaaggg cttcgatggc
     2281 gaggtggccg agcgcgctcg gctgttcttc tatctgccgt tcgaacatgc cgaagacagc
     2341 gccgcgcagg cgcgcggcgt cgctttggtg gcacggctcg ccgatcccga actcgtcaaa
     2401 tgggccgagg cgcaccaggc gatcatcgat cgcttcggcc gctttccgca tcgcaacgcc
     2461 gtcctaggcc ggctctcgac ggcacaggaa cgtgattttc ttgctcagcc ggggagttcg
     2521 ttctgaggct cgggggttgt caccagcgca cgtcgaggtt gacgtagggg acgcgcacct
     2581 tgacctggcc ggcgtcgggg tcgaccctga cgctgccgta ggggccctcg acgcggacct
     2641 tgccgcggtc cgcgttaacg gtgacgtcgg catagggcgt cgtcacgcga ctatcgcctg
     2701 gcggtgctgc cgcgggcgcc tcggccgtcg gcggcgagag gtccgccggg ggcgcctggg
     2761 cgtgaccgcc gccgggtgcg aaaacgccga cgccggccaa gaggccgacg ctaaggctcg
     2821 acgcgagaag cctccgggtg accatgaaca aacctctcat ccttgtcgcg gtggcgggac
     2881 cctctaacat ggttggcggc caaagcaacc gccagcgcgg cggagggtcg cggcggttgg
     2941 acctcgagtg ggccgcctct cggcctcaag aggtcatacg gctgtcgagg gcaattggcg
     3001 gcggttgccg cagcgtctga tagacgacgc aataccgctc ggtcagcttc atcagcgtcg
     3061 ctttctgctc ctcgctgagg tcgccgtcga tttcgaagct gaggcgaatg tcgcgaaagc
     3121 cgaccggcgc ctctttggca acgcccagcg tgccgcgaaa atcgaggtcg ccctccgccc
     3181 gaatgcgggc gtcgcgcaca gtgagctgca gcgccgtggc caccgcgttg agcgtgacac
     3241 cggcgcaggc gacaagggcc tcgagcagca tatcgccgga gcaggcgtgc atgcccgtgc
     3301 cccctgtcgc cgggtgcaag ccggcctcca cgagggcctt gcccgtttgt accgaacagg
     3361 tcacgccctc cccgagatga cctgcggcct tcagcgtgat cactgccgtc tcgggcgcct
     3421 cgcggtatct gtccttgagc ggggcctgca aggctttaag cgcggtggcg tccatcggtt
     3481 ttctccaagg gggctcgggt ctcgccgttc atagcacgcc ctcagggccg cgccagctgg
     3541 caggggtggc ggtgcgtgcg ccgcttgcgg tgggcggtgg acgggttcat ggtttcctcc
     3601 cgcaccggcc cggcgagcgc tggctcgccg gcggcgcgac gtttcgcatc gcccaggagt
     3661 ggcccatgcc cgcttccgtt gcccgtgcgc ctgcggcgac gtcactggca atcgagggcg
     3721 gccgatttct cctggtggga ggcgcaagcc tggtcggctc ggcaagcgcg caacgatttc
     3781 tggacgctgg cgctgccgag gttgtcgtct tcgacaatct atcgcaggga tcgttggccg
     3841 cgctccacca cctcgagggc aatccgcggc tgaagatcgt ggcgggagac gtgctgcgtc
     3901 tgccacaatt gctcgccgcc atgcgccatg tcgacggcgt tctccacttg gccgcgctga
     3961 tgtccctcag catggatcgc gatccctgga cgggcctcga cgtcaatatc cggggcacgc
     4021 aaaacgtgat cgaggcctgc tgtgtcattg gcgtcaagaa gctcgtgctg gcctcctcga
     4081 gtgccgccta cggttatggg ccgggcgtcg tcggcaagct gatcgagacg agcccctttc
     4141 actccagcgg cgccccgcca ggcgccattc tctatggtgc gagcaagatt atcggtgagc
     4201 agctctgccg cgacgtccac cgcaagcgcg gcctgaacta cgtcgtggtg cgctatgcaa
     4261 ccgtctatgg ggagcgtcag cattaccgtg ccgccaacgc gctctacatc atcgacacgc
     4321 acgaccgcgt gcgccgcggc gagcgcccgc gcgtgctggg ggacggttcc gaggtgaagc
     4381 acttcgtgca tgtcagcgat gtggcgcgcg ccaatgcagc ggcctttgcg tcggacgcca
     4441 ccgacgtggc cgtcaac
//
LOCUS       DASTMT010000542         5308 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_310741, whole genome shotgun sequence.
ACCESSION   DASTMT010000542 DASTMT010000000
VERSION     DASTMT010000542.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 5308)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5308)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5308
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_310741"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..475)
                     /locus_tag="VFR00_12435"
     CDS             complement(<1..475)
                     /locus_tag="VFR00_12435"
                     /inference="COORDINATES: protein motif:HMM:NF012862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HEU0160121.1"
                     /translation="MSKGMRLWRWLGASFALGVGMLLGGVAPTQAARHALLVGIGDYD
                     QKNNGMATLSAPAYDAEALGRVLGRTAFGFQVEVLVDQAAKDKVAFEAALQKFLGRVK
                     PGDEVLFYFSGHGVNLGDKGNYFILLDAKDQDAYIREQRRKPGSAREFDTQDKENK"
     gene            complement(643..1875)
                     /locus_tag="VFR00_12440"
     CDS             complement(643..1875)
                     /locus_tag="VFR00_12440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0160122.1"
                     /translation="MLPAKPIAIFPLTVAVCALALAGTGPVAAKTRNTIGTIIGVGAA
                     GALLNEAAKAMGQKPTRPGGPAAPGTSSSGETGKRNEDPDAAARAARQLAAIEENGEI
                     ERAKKMELERNVDLAIKAFIDKLIEWHNAINTGDRVRSTRGELNQVTAGQVKASIEEA
                     YDKANLREFEKYSGELWTRDRLLVRIMRYATKNLRPYYEGVGAKGPSMDDLKDLFHRS
                     AREAFAKALETGEIIGVSKSFDRFIRTIYENTDNGSENLTTRGMDGQYERLTSVAIDV
                     VWRNDFAAGAASSADSESLDRQFLFRFRSRRALYDCLSLTYPELLHGSGGTTTAAFTS
                     SSAAPSTERSRQSAAPRSLTLAEQEALAGKVRTHVANVCQGNVPTILAKAKSGNIGPV
                     SSRESGLATGTIPAAERR"
     gene            complement(2371..2769)
                     /locus_tag="VFR00_12445"
     CDS             complement(2371..2769)
                     /locus_tag="VFR00_12445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0160123.1"
                     /translation="MMPGAIGLGLLVAFAGASVWPAAPHPTPGGDLGTRLAQRLNPAP
                     VPGGLPGVAPMPLPAPAPQQAPSLPSPAAPVQVVPPPPPPRLSAPDAGGPEACDCQVE
                     VDVPIYEKGRIVRTQRERRVVGRGPQCCPK"
     gene            complement(2766..3860)
                     /locus_tag="VFR00_12450"
     CDS             complement(2766..3860)
                     /locus_tag="VFR00_12450"
                     /inference="COORDINATES: protein motif:HMM:NF017076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HEU0160124.1"
                     /translation="MGSILVLLGLFLVPAMAPALLRAEHWAADWRTAFLSDRLPSSHP
                     RVAIIPVTEASLTGLPYILPINRGYLADLVSAVDAAGARAIGLDFYFARETERPFDSK
                     LQGALQGAKAKIVLGAFEDASRPDQLAYQLQFITNSGAAAGYIDLAPEADHVVRYRAR
                     APRQAQHRESFSTVLARAAGWTGGAPPERISWLLPPADGKGIFEEIPAHTFLSAGPEQ
                     RSALAKDRIIIIGGKLFSLDRHFTPLSLRTGEGMSGVDIHAQMAAELVDGNRSYAELD
                     RASAQIFLGLLAALALVLGTRFQTRGFDFLNWRVASFLVIALDLVMFRFLRLMLPFTL
                     AAVAWIAGVTAGTQLRRALEWARSQWEGNA"
     gene            4430..>5308
                     /locus_tag="VFR00_12455"
     CDS             4430..>5308
                     /locus_tag="VFR00_12455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEU0160125.1"
                     /translation="MVTGAAALAAGTGLAPAFAQWRPSERYPDPAVEILDPSFAKYRL
                     TLASVERLATGMRWSEGPAWFGAGRYLIWSDIPNNRMMRWQEETGSVSVFRQPSNNAN
                     GNTRDRQGRLVTCEHDTRRLTRTEFDGSIITLIDKFDGKPLNSPNDVVVKSDDSIWFT
                     DPPFGVLGNYEGHVATPELPTNVYRLDPASGRATVAAGDVNRPNGLAFSPDESKLYIV
                     EAGVTPRVIKVYDVVDNGSKLANGRPFITAEPGGTPDGFRLDVDGNLWCGWGMGNEKQ
                     DGVKIFDATGKPIGFIA"
BASE COUNT          928 a         1737 c         1751 g          892 t
ORIGIN      
        1 gcttgttctc cttgtcctgg gtgtcgaatt cgcgcgcgct gccgggtttg cggcgctgct
       61 cacgaatgta ggcgtcctga tccttggcat cgagcagaat gaaatagttg cccttgtcgc
      121 cgagattgac gccgtggccg gagaagtaga acaagacctc atccccgggt ttgacgcgcc
      181 ccagaaactt ctgcaatgcc gcctcgaagg cgactttgtc cttggcggct tgatcgacga
      241 gcacttcgac ctgaaagccg aaggccgtcc gtcccaacac gcgccccagt gcttcggcgt
      301 cgtaggcagg cgcgctgagc gtcgccatgc cattgttttt ctgatcatag tcgccgatac
      361 ccacgagcag ggcatggcgt gccgcctgcg tcggcgccac cccgccgagg agcatgccca
      421 cgcccagcgc gaagctcgcg ccgagccaac gccaaagccg cattcccttg ctcatgcttg
      481 cctcattcga atcgaaattg aggagcggca cgccggtgaa gcgatgggcc agatgccccg
      541 agcacgccgt tcaatatcgc aagggctaga cacgcaactc gccccgggcg tcgttctctt
      601 gttggataaa gacccgcgcc tgccagctcc cgatgggttc tgttagcgcc gctcggccgc
      661 cgggatcgtc ccggtcgcca agcccgattc gcgcgaggaa accggcccga tgttaccgct
      721 cttggccttg gccagaatcg tcggcacgtt gccctgacag acattggcga catgcgtgcg
      781 gactttgccg gccagcgctt cctgctcggc gagcgtcagc gagcgtggcg cagcgctctg
      841 cctgctacgc tccgtgctcg gcgccgccga actgctcgtg aaggcggcgg tggtggtgcc
      901 tccgctaccg tgcaggagct ccggataggt cagcgacaaa caatcataaa gcgcgcggcg
      961 cgagcgaaag cgaaagagaa actggcggtc gaggctctcg ctgtccgcgg agcttgccgc
     1021 gccagccgca aagtcgttgc gccacaccac atcgatcgcc acgctcgtca gccgctcata
     1081 ctggccatcc atgccgcggg tcgtgaggtt ttcgctgcca ttgtccgtgt tttcgtagat
     1141 ggtgcggata aaccggtcga agctcttgct gacgccaatg atctcgccgg tttccaaggc
     1201 cttggcgaag gcttcgcgag cggatctgtg gaacagatcc ttcagatcat ccatgctcgg
     1261 gcctttggcc ccgacgcctt cgtagtaggg gcgtaggttt ttggtggcat agcgcatgat
     1321 gcgcaccagc aaccggtcgc gggtccaaag ctcgccgctg tatttttcaa actcgcgcaa
     1381 gttcgccttg tcgtaggcct cctcgatgga ggccttgacc tggccggcgg tgacctggtt
     1441 gagctcgcca cgtgtcgagc gcacgcggtc gccggtgttg atggcgttgt gccactcgat
     1501 caacttgtca ataaaggcct tgatggcgag gtcgacgttg cgctcgagct ccatcttctt
     1561 agcgcgctcg atttcgccgt tttcctcgat ggcagcaagc tgtcgagcgg cgcgcgcggc
     1621 ggcgtcggga tcttcgttcc gcttgcccgt ctcgccgctg ctcgaggtcc cgggcgcggc
     1681 ggggcccccc ggccgcgtcg gcttttgccc cattgccttg gcggcctcgt tcaggagggc
     1741 gccggcggcg cccacaccga tgatcgtgcc gatggtgttg cgggtctttg ccgcaaccgg
     1801 cccggtgccg gcgagcgcta gcgcgcagac agcgacggtg agcggaaaaa tagcgattgg
     1861 cttagccggc agcatgatga cgtcccccgt ggcgtgcaaa atgcagaagg cggataaacg
     1921 cgtaaagcct agtgtattcg gttacccgaa gcaagatcga gggcgggagc ggtggattca
     1981 gttgccgccg tcgggcgttt tcttttggcc ccgcggggct ttcttgtcgg gcttttcagc
     2041 cgctgccgga gcctcctccg agcgcccagg ggatttaggc gtttgcaggg agcgcgtcga
     2101 cggtggatcg gcttgccgac tgcgtccggg cacgcctttg gcacccggcc gcccttcctc
     2161 ttccccttcg ctcatgccgg ggagaccgcc tggctggcgc tgcgccaggt gcagctgcgg
     2221 gcgccaaagc ggcgccccca cctcggttgt gggatcgcgc ggcgcggcgg acgctgggag
     2281 caacgggacg acgacgcagg ggaacagcga aagtaaaaac aacgcggaac gcatggacac
     2341 ctcttaatca cgagaccggc gctagccgca tcacttcgga caacactgcg ggccgcggcc
     2401 caccacgcgc cgctcgcgtt gcgtgcgcac gattcggccc ttttcgtaaa tcggaacgtc
     2461 gacctccacc tggcagtcgc aggcctcggg tcccccggca tccggggcgc tgagccgggg
     2521 cggcgggggc ggcggcacga cctgcaccgg cgccgccggc gaaggcaggc tgggcgcttg
     2581 ctgtggcgcg ggcgcgggca gcggcatcgg cgccaccccc ggcaagccgc cgggaaccgg
     2641 cgccgggttc aaacgttgcg ccaagcgtgt gccaaggtcg ccgccgggcg tgggatgcgg
     2701 cgccgccggc cacactgagg cgccggcgaa agcgacgagg aggccgaggc cgatcgcgcc
     2761 aggcatcatg cgttcccctc ccattgcgag cgcgcccatt cgagcgcacg ccgcagttgc
     2821 gtccccgccg tcacgccggc aatccaagcc accgccgcaa gcgtgaaggg gagcatcagc
     2881 cgcagaaatc ggaacatcac gagatcgagg gcgatgacaa ggaagcttgc aacccgccaa
     2941 ttgagaaagt cgaagcctcg cgtctggaac cgggtcccca gcaccagggc gagcgcggcg
     3001 agcaggccca agaagatctg cgcgctggcg cggtcgagtt cggcataaga ccggttgccg
     3061 tcgaccagtt ctgccgccat ttgcgcatga atatcgacgc cgctcatgcc ctcaccggta
     3121 cgcaaggaca gcggtgtgaa gtgtcgatcg agcgagaaaa gcttgccgcc gatgatgatg
     3181 atgcgatcct ttgccagcgc gcttctttgt tcaggcccgg cgctgagaaa ggtatgcgcg
     3241 ggaatctctt cgaagatgcc cttgccgtcc gcgggtggca gcagccagga aatgcgctcc
     3301 ggcggcgcgc ctcccgtcca gccggctgcc cgcgccagca ccgtggagaa gctctcccga
     3361 tgctgggcct ggcgcggagc gcgcgcacga tagcgcacga cgtgatcggc ctcgggcgcc
     3421 aggtcgatgt agcccgccgc ggcaccgctg ttggtgatga actggagctg ataggcgagc
     3481 tggtccggtc gggaggcatc ctcgaatgca ccaagcacga ttttggcctt ggcgccctgc
     3541 agggcacctt gcagcttgct gtcgaagggg cgttcggtct cgcgggcgaa atagaagtcc
     3601 aagccgatgg cgcgcgcgcc ggcggcatcc accgcgctca ccaagtccgc aaggtagccg
     3661 cgattgatcg gcagaatata gggcaaccca gtcagcgaag cctcggtcac cgggataatg
     3721 gcgacgcgcg ggtgtgagct cggcaagcgg tccgacaaaa aggccgtgcg ccaatcggcg
     3781 gcccagtgct cggcgcgcaa cagagccggg gccatggccg gcaccaggaa cagccccaac
     3841 agcacgagaa ttgagcccac gcacagcgcg aaatggagcc acgagggtgc ggccccgacg
     3901 gtcgtggaag aagaaatcgc ccgcacttgc gcccccgagc ggccgagcga agggccttgg
     3961 ccagcgagga actctcagcc gagatcgggg ccgcgtcaac ccgtgtcgca gcgagggcgc
     4021 cgcaagcgtg gcaaacctgc agagggcgca cccgcttgcg ccgcgcagag ccgcagcgtt
     4081 tgtgcgcttt gcgatgccgg gcgggctgct gcaggcagcc gtgctcggcg tgcggccggt
     4141 gatcgggcag catctcgacc tccaggcgca gtcttgagga cgacgcgggg gagatccaaa
     4201 gaggcccttg tcgcaaatgc cccggcccgc gccgccgcca ggcaaggctc ttacagcgcg
     4261 agcgacgcgt aggccgcggg cctcagcggc aaaacattct ttttgcttgt ctggcagccg
     4321 gccgccaggg aagaattgtg ccgaagcagg cgccacctcg ccgacagcca tgggtattgc
     4381 gcggggcgag caacccggag gccagtcaat gacgacgcga cgtgagttca tggtcacggg
     4441 tgccgccgcc ctggccgccg gcacgggcct ggcgccagcc ttcgcgcaat ggcgtccgag
     4501 cgagcgctat cccgacccgg cggtggagat cctggatccg agcttcgcca aataccgcct
     4561 gacgctggcc agcgtcgagc gtcttgccac cggcatgcgc tggagcgaag gtccagcctg
     4621 gtttggcgcc ggccgctatc tgatctggag cgatattccc aacaaccgca tgatgcgttg
     4681 gcaggaggaa accggcagcg tcagcgtatt ccgccagccc tccaacaatg ccaatggcaa
     4741 cacccgagac cggcagggcc gcctcgtcac ttgcgaacac gacacccgtc gcctgacgcg
     4801 caccgagttc gacggcagca tcatcacact gatcgataag ttcgacggca agccgctcaa
     4861 ttcgcccaat gatgtcgtgg tcaaatcgga tgactcgatt tggtttaccg atccgccgtt
     4921 cggcgttctc ggcaactacg agggtcacgt cgccacaccc gaacttccca ccaacgttta
     4981 tcggctcgat cccgccagcg gccgagccac cgtggcggcc ggcgatgtca atcgcccgaa
     5041 cgggctcgcc ttctcaccgg atgaatcgaa gctttatatc gtcgaggccg gcgtcacgcc
     5101 gcgggtaatc aaagtttacg acgtcgtcga caacggcagc aaacttgcga acggacgccc
     5161 cttcatcacc gccgagccgg gcggtacccc cgacggtttc cggctcgatg tcgacggcaa
     5221 cctctggtgc ggctggggca tgggcaacga gaagcaggac ggggtgaaga tcttcgatgc
     5281 cacgggtaag cctatcggct ttatcgcg
//
LOCUS       DASTMT010000543         2962 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_971750, whole genome shotgun sequence.
ACCESSION   DASTMT010000543 DASTMT010000000
VERSION     DASTMT010000543.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2962)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2962)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2962
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_971750"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..705)
                     /locus_tag="VFR00_12460"
     CDS             complement(<1..705)
                     /locus_tag="VFR00_12460"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEU0160126.1"
                     /translation="MRVGWLDGVPQRGLVLLLLLLLALILLPAFASDYLITVLILILY
                     FAYVGQAWNLMMGLAGQLSLGHALYVGLGAYAAAALYVHFGVSPWIGLWASLAIAAAA
                     GGAIGFLAFRFGVAGVYFAILTIAFAEFARLAFDHWPWLGGSAGFFLPVANYARNDLL
                     NLRGSPTMFYYLLLAMTAGAFVLCHALLRSRAGYYWLAIRESPDAAQALGIDLFRYRM
                     YVVVLSAAMTSLGGVVF"
     gene            complement(702..1574)
                     /locus_tag="VFR00_12465"
     CDS             complement(702..1574)
                     /locus_tag="VFR00_12465"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEU0160127.1"
                     /translation="MPASIYLNVAVSGLLTGLVYGLMALGLSVIFGVVRVVNFAHGEM
                     MTIAMYIAVALFAAYRLDPLLMLVPIAAVLFALGYVMQAGLINPFINRPEHSQFMLLV
                     AVAMIIVNTLLLVFGPDARSVQTTYAYDSFQLGPLIVDATKVYAAVAAAVVAGGLFAF
                     FRYSLTGKAIRACADNYQGALVVGLDVRRLYALTFGLGAACVGAAGSMLVLLLDVTPA
                     LGPSYTLLAFVIVITGGLGSMPGALLGGMLIGVTEALAGLLFTPSAKSMFAFGMLVLV
                     LLFRPQGILGKSAS"
     gene            complement(1636..2880)
                     /locus_tag="VFR00_12470"
     CDS             complement(1636..2880)
                     /locus_tag="VFR00_12470"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0160128.1"
                     /translation="MSRSMGASVSGLNRRSLAKGLGAGIIAGVAPFHIGRAQGAGLKV
                     GVLLPRSGVQAGIGQDCQRGVEIAAGILKDIGLPDLAIMSGDTESNVETARSRAERLI
                     GEGAQLLVGAFDSGQSTAIAQVAEQKGIPFVINIAAAPPITEQGYKFVFRNFPTAGMI
                     LGDAFANQKAIFEATGSAPQSVVFMHVNDTFGVSMQKGIDAVLPKFNMPYKILDTIAY
                     DPAARDLSVEVAKAKATGAEALLTVSRLNDAILLTRELVKQRWSPLAILSMGPGWYED
                     QYIKTLGKLSDDPLSFVPWYDPNKKLSKQLEDALAKAHPGINLNTNHIYTFEALLIAA
                     DAYKRAASSDPKALADAIRTTDITQNVSIGPGIKFDAKGQNDKLKNAGIQNRGGKLLT
                     VAPMAAANAKVVWPLTAYDKRA"
BASE COUNT          612 a          918 c          966 g          466 t
ORIGIN      
        1 aaagacgacg cctcccaacg atgtcatggc cgccgatagc acgaccacat acatcctata
       61 acggaagaga tcgatgccga gcgcctgggc ggcgtcgggg ctctcgcgga tcgccagcca
      121 gtagtagccg gcgcggctgc gcaacagcgc gtggcaaagc acgaaggcgc cggcggtcat
      181 cgccagcagc agatagtaga acatggtcgg gctgccgcgc aggttgagga ggtcgttgcg
      241 cgcatagttg gcaacgggca agaagaaccc ggctgagccg cccagccagg gccagtggtc
      301 gaaggccaga cgggcgaatt cggcgaaggc aatggtgaga atggcgaagt agaccccggc
      361 gaccccgaag cggaaggcga gaaagccgat ggcaccgccc gctgcggcgg caatcgccag
      421 cgacgcccac aggccgatcc acgggctcac gccaaagtgc acgtaaagcg cggcggcggc
      481 gtaggcgcca agaccgacgt agagcgcatg gccaagcgaa agctgcccgg cgagacccat
      541 catcaggttc cacgcctgcc cgacgtaggc gaaatagagg atgagaatca gcaccgtgat
      601 caggtagtca ctggcaaaag cgggcaacag gatcagcgcc aagaggagca gcaggagaag
      661 gacaagccca cgctgaggaa cgccgtcgag ccaacccacc ctcatgacgc gctcttgccg
      721 agaatgccct gcggccggaa cagcagcacg agcacgagca tgccgaaggc gaacatgctc
      781 ttggccgaag gtgtgaacag caagccggca agcgcctcgg tcacgccgat cagcatcccg
      841 cccagaagcg cgcccggcat tgaaccgagg ccgccggtga tgacgatcac aaaagccagg
      901 agcgtgtagc tggggccgag cgccggcgtc acatccaaca gcagcaccaa catggagccg
      961 gccgccccga cgcaggccgc gccgagcccg aacgtcagcg catagagccg ccggacgtcg
     1021 aggccaacca cgagcgcgcc ttgatagtta tcggcgcagg cgcgaatggc cttgccggtc
     1081 agcgagtagc ggaagaacgc gaacaggccc cccgccacca ctgcggcggc aacggcggca
     1141 tagaccttgg tggcatcgac gatcaacggc cccagctgga agctgtcata agcataggtc
     1201 gtctgcacgc tgcgcgcatc cggtccgaag acgagaagca gcgtgttgac gatgatcatc
     1261 gccacggcca ccagcaacat gaactggctg tgctccggtc gattgatgaa aggattgatc
     1321 agaccggcct gcatgacata gccgagcgcg aacagaaccg cggcaatcgg caccagcatc
     1381 aacagcgggt cgagccggta ggcggcaaag agcgcaacgg cgatgtacat cgcgatcgtc
     1441 atcatttcgc cgtgcgcgaa attgaccacg cgcaccacgc cgaaaatgac ggacagcccg
     1501 agcgccatca ggccataaac caggccggtg agaaggccgc tgacggcaac gttcaaatag
     1561 atacttgcgg gcaccggcag cggtcctggc aaaaagccgc tccggctcgg ccggagcgct
     1621 ggagctagac cacgatcaag cgcgcttgtc gtaggcggtc aagggccaaa cgaccttggc
     1681 gtttgccgca gccatgggag cgaccgtcaa gagcttgccg ccgcggttct ggatgccggc
     1741 attcttgagt ttgtcgtttt ggcccttggc gtcgaacttg atgcccggcc cgatgctgac
     1801 gttttgcgta atatcggtgg tgcggatggc gtcggcgagc gccttggggt cgctcgaagc
     1861 cgccctcttg taggcgtccg cagcaatcag cagcgcctcg aaggtataaa tgtggttggt
     1921 gttgagattg atgccgggat gggccttggc cagggcatcc tcgagctgct tgctcagctt
     1981 cttattggga tcataccagg gcacaaagct cagcgggtcg tcggagagct tgccgagcgt
     2041 cttgatgtat tggtcctcgt accagcctgg gcccatgctc aaaattgcca gcggcgacca
     2101 gcgctgcttg acgagctcac gcgtgagcag gatggcatcg ttcagtcggc tgaccgtcaa
     2161 aagggcctcc gccccggtgg ctttggcctt ggcgacctcg accgacagat cgcgtgcggc
     2221 cggatcgtag gcgatggtgt cgaggatctt gtagggcatg ttgaacttgg gcaggacggc
     2281 gtcgataccc ttctgcatcg acacgccgaa ggtgtcgttc acatgcatga acacgacgga
     2341 ctgcggcgcg ctgccggtgg cctcgaagat cgccttctga ttggcgaacg cgtcgccgag
     2401 gatcatcccc gccgtgggga agttgcggaa tacgaacttg tagccctgct cggtgatggg
     2461 gggcgccgcc gcaatattga tcacgaaggg aatacccttt tgctcggcca cctgggcgat
     2521 tgccgtggat tgaccggagt cgaaggcgcc aaccaacagt tgcgcgcctt ccccgatcag
     2581 gcgctcggcg cgcgagcggg cggtctccac gttggattcg gtatccccgc tcatgatcgc
     2641 aaggtccggc aggccgatgt ccttcagaat gcccgcggcg atttcgacgc cgcgctggca
     2701 gtcctgacca atgcccgcct gcacaccgga tctcggcagc aagacgccga ccttgaggcc
     2761 ggcgccctgg gcgcgcccga tatgaaaggg ggctaccccc gcaataatcc cggccccgag
     2821 acccttggcg aggctgcggc ggttgaggcc gctcacgctc gcccccatgc tccggctcat
     2881 gcgcgttctc ccttcggtgt gcggattttg tctgcttgaa gagtatatgg cttttgcccg
     2941 ccctgcgtca ttcctctcgg gc
//
LOCUS       DASTMT010000544         2767 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_5735380, whole genome shotgun sequence.
ACCESSION   DASTMT010000544 DASTMT010000000
VERSION     DASTMT010000544.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2767)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2767)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2767
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5735380"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            144..998
                     /locus_tag="VFR00_12475"
     CDS             144..998
                     /locus_tag="VFR00_12475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01459 family HAD-type hydrolase"
                     /protein_id="HEU0160129.1"
                     /translation="MSGPSIPILSSIGSFADQSEAWIVDIWGVLHNGAHAFAPAAEAC
                     AKFRQQGGVVVLLSNAPRPFTAVVSQLDALGVARTAYDAGVTSGDVTRAMIAPWQGRP
                     VLHIGPARDQGLFDGFVLDFADAGRAEIIVCSGLNDDSKETPQHYTELFAGLAARGVA
                     MICANPDLVVERGDKLVYCAGALAAEYARLGGTVSYAGKPHWPVYERALAEIDTRSGR
                     PIAKTRVLAIGDGIDTDLLGAHRAGLRSVFIASAVHVPEGLSGATLSRLFAARPFTPI
                     AALAALRW"
     gene            complement(999..2000)
                     /locus_tag="VFR00_12480"
     CDS             complement(999..2000)
                     /locus_tag="VFR00_12480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018387705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HEU0160130.1"
                     /translation="MPSPPETMTAIEISAPGGPDVLKPKVVPTPQPGAGQVLVKVAAA
                     GVNRPDLAQRSGSYPPPAGASPLPGLEVAGEVVRVGEGVARWRLGEQVCALVNGGGYA
                     EYCVVEDVQALPIPAGHDLITAAAIPETFFTVWANVFERGRLAAGEWFLVHGGSSGIG
                     TTAIQLAKAFGAKVIATAGSAEKCQACRELGADRAVNYRSEDFVKACQEATGGQGVDL
                     ILDMVAGAYTERNILAAADDGRIVQIAVLGGANVSINIARVMMKRLTLTGSTLRPRSR
                     AFKGSLAAALEKQVWPLLASGTVRVVLDATFPLRKAADAHRHLEAGQHIGKVVLTVA"
     gene            2117..2305
                     /locus_tag="VFR00_12485"
     CDS             2117..2305
                     /locus_tag="VFR00_12485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1192 domain-containing protein"
                     /protein_id="HEU0160131.1"
                     /translation="MDWDELKPTPTKGITLGESLANLSISELEARVVALAAEIERVQE
                     NIAAKKAHEAAAAAVFKR"
BASE COUNT          451 a          913 c          922 g          481 t
ORIGIN      
        1 cgtgggcgag cctgccgcgg cttgatcgcg ccggcgcttg ccgctagagc acgggcgtgg
       61 cgcccccggc gagcgggggc ggtcccttcg ctcccgcaga ggcaacgtgg ccccatcgcc
      121 agtaatcccg ctttagggcg cgcatgagcg ggccaagcat ccctattctg tcctcgatcg
      181 gcagcttcgc cgatcagagc gaggcctgga tcgtggacat ctggggcgtg ctgcacaatg
      241 gcgcgcacgc ctttgcgccc gcggcagagg cctgcgccaa attccgccag cagggcggcg
      301 tcgtcgtgct gctgtcgaat gcgcctcgcc cgtttacggc cgtcgtgtcg cagctcgatg
      361 cgcttggggt ggcgcgcacg gcctatgatg ccggcgtcac ctcgggggac gtgacgcgcg
      421 cgatgatcgc gccctggcaa ggccggcccg tgctgcacat cggtccggcg cgcgaccagg
      481 gcctattcga cgggttcgtg ctcgactttg ccgacgccgg ccgcgccgag atcatcgttt
      541 gctcgggctt gaacgacgac agcaaagaga cgccgcagca ttataccgaa ctgttcgcag
      601 gcctggcggc gcgcggggtg gcgatgatct gcgccaatcc cgacctcgtc gtcgagcgcg
      661 gtgacaagct cgtctattgc gccggcgcgc ttgccgccga gtatgccagg ctcggcggca
      721 ccgtcagcta tgcgggaaag ccgcactggc cggtctatga acgcgcgctg gctgagatcg
      781 atacccgcag tggccgcccg atcgccaaaa cccgggtgct cgccatcggt gacggtatcg
      841 acaccgacct attgggggcg catcgggcgg gactgcgctc ggtgttcatt gccagtgccg
      901 tgcacgtccc cgaagggctc agcggcgcca cgctcagccg tttgttcgcg gcgcgtccat
      961 tcacgcccat cgccgcactc gccgccttgc ggtggtagtt acgccacggt cagcacgacc
     1021 ttgccgatat gctggccggc ttcgagatgg cggtgcgcgt cggcggcctt gcgcagggga
     1081 aaggttgcat cgagcaccac ccgtaccgtg ccggaggcga gcaagggcca gacctgcttc
     1141 tccagcgcgg cggccagcga acccttaaag gcgcgcgagc gcgggcgcag ggtggagccc
     1201 gtcagtgtca gacgtttcat catcacgcgc gcgatgttga tgctgacgtt ggcgccgccg
     1261 agcacggcga tctgcacgat gcggccgtcg tcggctgccg cgaggatgtt gcgctcggtg
     1321 taggcgccgg cgaccatgtc gaggatcaga tccacacctt ggccgccggt tgcctcctgg
     1381 caggctttga cgaaatcctc gctgcgatag ttgacggccc ggtcggcccc gagctcgcgg
     1441 cacgcctggc atttctccgc cgagccggcg gttgcaatca ccttggcgcc aaaggccttg
     1501 gcgagctgaa tggcggtggt gccgatgcca ctggagccgc cgtgcaccag gaaccattcg
     1561 ccggccgcca ggcggccgcg ctcgaacaca ttggcccaaa ccgtaaagaa ggtttccggt
     1621 atggctgcgg ccgtgatgag gtcgtggcca gccggaatcg gcaaggcctg tacatcctca
     1681 accacgcagt attcggcata gcctccaccg ttgacgaggg cgcagacttg ctcgcccagg
     1741 cgccagcgcg cgacgccttc gcccacgcgc accacctcgc ctgcaacctc gaggccgggc
     1801 agcggcgaag cacccgctgg cggcgggtaa gagcctgatc gctgcgcgag gtcggggcgg
     1861 ttaacaccgg ccgcggccac tttcaccaac acttgcccag cgcccggctg aggtgttggt
     1921 accaccttgg gcttgagcac gtccgggcca cccggggcgc tgatctcgat cgccgtcatc
     1981 gtttccgggg gggaaggcat ggttcagctc cgcgcaggat tgggcaggtc ggacggcaga
     2041 gtggcccggc gcgcggggtt gagacaaggg cgaactcgtg ccaggctcga ggtcccattt
     2101 gcgcttcgag gtggccatgg attgggatga gctcaaaccg acaccgacaa agggcatcac
     2161 gctcggggag tcgctcgcca acctatcgat cagtgaactg gaggcccgcg tcgtagcgct
     2221 cgcggccgag atcgaaaggg tgcaggagaa tatcgcggcc aaaaaggcgc acgaagccgc
     2281 ggccgcggcc gtattcaaac gctgagccca agggcaaaga agtagctaca agccttttcg
     2341 aaccgcacgc gccccgcagc gccggcgcgg gcgccacgtt cgatttgggg gcggcgcttt
     2401 accttttgtt atccaatcag ttgcatcatc gggttatcca gttctctgga tccgagtgga
     2461 tcctgtccac tccgtttgac gcctccctgt taactcctga gccgccgcac ggcggctcat
     2521 ttttcttatc tataggtgtc tttcctaatt cgaatgagcg cgccctcgcc accgatccgc
     2581 gcccgagcgg caggcgtggc cttgtaaagg accctgtgcg cggcttcagc caccgctttc
     2641 gaattatgtt cgagcgcgcg cccccgcgct cgcgcaagcg aagaccaagt tgaaataaga
     2701 tgtgcccggt cgcttcggct tggcagaaga tgtgcataaa gcacgtgtgc gccacaccac
     2761 agtcgca
//
LOCUS       DASTMT010000545         3335 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_1624887, whole genome shotgun sequence.
ACCESSION   DASTMT010000545 DASTMT010000000
VERSION     DASTMT010000545.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3335)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3335)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3335
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1624887"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..669
                     /locus_tag="VFR00_12490"
     CDS             <1..669
                     /locus_tag="VFR00_12490"
                     /inference="COORDINATES: protein motif:HMM:NF023176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3306 domain-containing protein"
                     /protein_id="HEU0160132.1"
                     /translation="HAVTGPQNPSEPFLARWSRLKDKERQPAPTPAEPAAPPTPTHAD
                     PQAPAQQTGDAPPAAIDLASLPPLDSIGAGSDIRAFLQSGVPAELTKAALRRAWTSDP
                     AIRDFIGIAENQWDFTDPAAIPGFGPIEANTDLAALVRQALGEIAPSAQDHAGASPSV
                     AAAAEPSAQPYGIPDRNADTGRADGVVEVQHSARCAAAQHADASAVAAPAPSRRSHGG
                     ALPK"
     gene            826..1599
                     /locus_tag="VFR00_12495"
     CDS             826..1599
                     /locus_tag="VFR00_12495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019400970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD family protein"
                     /protein_id="HEU0160133.1"
                     /translation="MRDHGLQEAIRAAGGIGELARRLGISQPSVSNWDRIPAERVLSV
                     EQATGVARALLRPDLFGDRARETLDEVDAARAREYALLASLIGRSPDAAFLKRLAQLR
                     GDATPLGLAHAALGDAAERAEAAEVGREYFDLFIGLGRGELLPYASYYLTGFLYERPL
                     ARLRADLKRLGIEAEPGQSEPEDHAAILCEIMASLIGKETAAPANADQEIFQKHLAPW
                     IGRFFTDLERSPTAGFYASVGALGRTFIEIETEAYALPA"
     gene            1638..1844
                     /locus_tag="VFR00_12500"
     CDS             1638..1844
                     /locus_tag="VFR00_12500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016842205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase"
                     /protein_id="HEU0160134.1"
                     /translation="MATDKAKLGRRDVLRVLGGGAGLAAASGTLATGARADSETNEEK
                     RKSRYRETEHVKTFYRVNRYPTKR"
     gene            1856..>3335
                     /locus_tag="VFR00_12505"
     CDS             1856..>3335
                     /locus_tag="VFR00_12505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014491721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEU0160135.1"
                     /translation="MLIKKSERHARRGGLVAALAGQSGGGLDRRAFLRRSGLAAGSLA
                     ALGTLPLGGIRKAEAAGPAPTSGIIRQNMCTHCSVGCTVMAEVENGVWVGQEPGWNSP
                     INRGSHCAKGAATREIAHGERRLRYPMKIVGGQWQRISWDTAINEIGDKLLAIREKSG
                     PDSVYWLGSAKFTNEASYLNRKLAAFWGTNNSDHQARICHSTTVAGVANTWGYGAMTN
                     SYNDIRNAKTMIIMGGNPAEAHPVSLQHVLEGKELNRANMIVIDPRLTRTAAHATEYV
                     RIRSGTDIPVIYGMLWHVFKNGWEDKEFIKQRVYGMDDVRKEVDKWTPEEVERVTGVP
                     GAQLERVAKMFATEKPATLIWCMGATQHTVGTANVRAFCIALLATGNVGKFGTGANIF
                     RGHTNVQGATDLGLDIVTLPLYYGLAEGAWKHWCRVWEVDFGWMEGRFDTITTADGKP
                     VKMMNTPGIPSTRWFDATLLPKDQVSQKDNLKAMVIMGHGGNT"
BASE COUNT          619 a         1132 c         1064 g          520 t
ORIGIN      
        1 cacgccgtga cagggccgca aaacccctca gaaccctttt tggcgcgctg gtcgcggctg
       61 aaagacaagg agcgccagcc cgccccgacg ccggccgaac ccgccgcccc accgacgccc
      121 acccacgccg atccccaggc gccggcgcaa caaacggggg atgctccccc cgcggccatc
      181 gatctcgcca gcctgccgcc gctcgacagc attggggcgg gcagtgacat ccgggcgttc
      241 ctgcagtccg gggtcccggc cgagctcacc aaggccgcgc tgcggcgcgc ctggaccagc
      301 gaccccgcca tccgcgattt tatcggcatc gccgaaaacc agtgggactt taccgacccc
      361 gctgccatcc ccgggttcgg cccgatcgag gccaataccg atctcgccgc gctcgtgcgc
      421 caggcgctgg gcgagatcgc gccctcggcc caggaccatg caggcgccag ccccagtgtg
      481 gccgctgccg ccgaaccttc agcgcaacca tatggtatac cagacagaaa tgcagacacg
      541 gggcgtgccg acggagtagt agaagtgcaa catagtgcgc gttgtgctgc agcgcagcat
      601 gccgacgcct cagcagtggc agcgcctgcg cccagtcgac gcagccatgg cggcgcactg
      661 ccgaagtaaa acggcccacg gtagtcttgg ccaatgttgt gctcctatag tctcatcctt
      721 tgggccactt ccccatagcc ctaagctatt gacgctggtg ttgcagaagc attagggtct
      781 ggaaccgttc cattccgagg cgccacgcgc ctaactctta ggcacatgcg ggaccacggt
      841 ttacaggaag cgatccgcgc cgccggcggc attggcgaac tcgcgcgccg cttggggatc
      901 tcgcagcctt cggtttcaaa ttgggatcgt attcccgcgg agcgcgtgct ttcggtcgag
      961 caggcgacgg gcgttgcgcg ggccttactg cggcccgacc tgttcggcga tcgagcgcgg
     1021 gaaaccctcg acgaggtcga tgcggcgcgc gcccgcgaat atgccctgct cgccagcctg
     1081 atcggccgca gccccgatgc ggctttcttg aagcggctgg cgcagctgcg cggggatgcg
     1141 acgccgttgg gtctggcgca cgccgcgctg ggggacgccg ccgagcgcgc cgaggcggcc
     1201 gaggtcggcc gggaatattt cgatctcttc attggtctcg gccggggcga gctgttgccc
     1261 tacgcctcct actatctgac gggctttttg tacgagcggc cgctggcgcg cctgcgcgcc
     1321 gacctcaagc gcctcggcat cgaggccgag cccggccagt ccgagcccga agatcatgcc
     1381 gccatcctct gcgaaatcat ggcgagcctg atcggcaagg agacggcggc gccggccaac
     1441 gccgaccagg agatattcca gaagcatttg gcaccctgga tcggccgttt cttcaccgat
     1501 ctggaacgtt cacccacggc cggcttttac gccagcgttg gcgcactggg ccggaccttt
     1561 atcgagattg agaccgaggc ctacgcgctt ccggcctagt gccgcaggca ggctcgtcgg
     1621 aggaggagga gacattcatg gcaacggata aagccaaact cgggcgccga gacgtcttgc
     1681 gtgtgctcgg tggcggggcg ggcctcgccg cagcaagcgg cacgctcgcc acgggcgcgc
     1741 gcgcggacag cgagaccaac gaagaaaagc gtaaatcccg ctaccgcgag accgagcacg
     1801 taaagacatt ctaccgggtc aacagatacc caaccaaacg ctagggaggc cggctgtgct
     1861 gatcaagaag agcgaacgcc acgcgcgtcg cggcgggctt gtcgcggccc ttgcgggtca
     1921 atccggtggc ggcctcgacc gccgcgcctt cctgcgccgc tccgggctgg cggcggggag
     1981 ccttgccgcc ctcggcacct tgccgctcgg cgggatccgc aaagccgagg cggccggccc
     2041 cgcgccgacc tccggcatca tccgccagaa catgtgcacg cactgttcgg tcggctgtac
     2101 cgtgatggcg gaggtggaaa acggcgtctg ggttgggcag gagcccggct ggaacagtcc
     2161 catcaaccgc ggctcgcatt gtgccaaggg tgccgccacg cgcgagatcg cgcacggtga
     2221 gcggcgcctg cgctacccga tgaagatcgt gggcgggcaa tggcagcgca tctcctggga
     2281 taccgccatc aacgagatcg gcgacaagct cttggccatt cgcgagaagt cgggtcccga
     2341 ttcggtctat tggctgggtt cggccaaatt caccaacgag gcctcctacc tcaatcgcaa
     2401 gctcgccgcc ttttggggca ccaacaactc cgaccaccaa gcccgcatct gtcactccac
     2461 cacggtcgcc ggcgtcgcca atacctgggg ctacggcgcc atgaccaaca gctacaacga
     2521 catccgcaac gccaaaacca tgatcatcat gggcggcaac ccggcggagg cccaccccgt
     2581 ctccttgcag catgtgctgg aaggcaagga actgaaccgg gccaacatga tcgtgatcga
     2641 tccgcgcctg acgcgcacgg cggcgcacgc caccgaatac gtgcgcatcc gctcgggcac
     2701 ggatatcccc gtcatctacg gcatgctctg gcatgtcttc aagaatggct gggaggacaa
     2761 ggaattcatc aaacagcgcg tctacggcat ggacgatgtc cgcaaggagg tcgacaagtg
     2821 gacgcccgag gaagtcgaac gcgtcacggg cgtgcccgga gcacagctcg aacgcgtcgc
     2881 caagatgttc gccaccgaaa agccggcgac gctgatttgg tgcatgggtg cgacccagca
     2941 cacggtcggc accgccaacg tgcgcgcctt ttgcatcgcg ctgcttgcca cgggcaacgt
     3001 tggcaagttc ggcacggggg ccaacatctt ccgcggccac accaacgtgc aaggtgccac
     3061 cgacctcggc ctcgatatcg tgacgctgcc gctctactac ggcttggcgg aaggcgcctg
     3121 gaagcactgg tgccgcgtct gggaggtcga cttcggctgg atggagggcc gcttcgatac
     3181 catcacgact gccgacggca agcccgtcaa aatgatgaat acgccaggca ttccatcgac
     3241 gcgctggttc gacgccacgc tgctgccgaa ggatcaggtg tcgcaaaagg acaacctgaa
     3301 ggccatggtg atcatgggcc atggcggcaa cacgg
//
LOCUS       DASTMT010000546         1504 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_2937454, whole genome shotgun sequence.
ACCESSION   DASTMT010000546 DASTMT010000000
VERSION     DASTMT010000546.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2937454"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..288
                     /locus_tag="VFR00_12510"
     CDS             <1..288
                     /locus_tag="VFR00_12510"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEU0160136.1"
                     /translation="VARYGGEEFAVILPGAELEDAKALTEHLRRQLETKKLAVNKSGQ
                     EIGTITASFGIAQLSVGEDAQKLVQRADAKLYEAKCAGRNRVVAAERSVAS"
     gene            408..1436
                     /locus_tag="VFR00_12515"
     CDS             408..1436
                     /locus_tag="VFR00_12515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006020211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HEU0160137.1"
                     /translation="MIPNAYRRVVLVRRPPGEPAEADFRIEQVPMPEPGPRQILVRNI
                     YMSLDPYMRGRMREAKSYAAPVAIGEVMTGGTVGEVVKSNHPDYKVGDIVEDRLGWQE
                     YGVGPSAAARKVDPTLAPISTANGVLGMPGMTAYFGLFDVGRPKAGETVVVSAASGAV
                     GQIVGQLAKLAGCRAVGIAGGPQKCAFVKDALGFDACIDYKAGNDLGEALKAACPDGV
                     DVYFDNVGGAVSDAVFLNLNFWARVALCGSISQYNVAAPELGPRLLGLFVGRRVNMRG
                     FIVWDFNGQYGPAMRQMGEWVRSGRLKYKEDIVEGLEQAPGAFIGLLRGENFGKLQVK
                     LGPDPTAR"
BASE COUNT          251 a          492 c          494 g          267 t
ORIGIN      
        1 gtcgcccgct acggcggtga ggaattcgcc gtcattctgc cgggcgccga gctcgaggac
       61 gccaaggcgt tgaccgagca tctgcgccgc cagctcgaaa ccaagaagct cgccgtcaac
      121 aagagcggcc aggaaatcgg cacgatcacc gcctcgttcg gcattgccca gctcagcgtc
      181 ggcgaggatg cccaaaagct cgtgcagcgg gccgatgcca agctgtatga ggccaagtgc
      241 gccgggcgca accgcgtggt tgccgccgaa cgctcggtcg ccagctgacc ggcgcgggcg
      301 agcgccttcc tccagcctcg catgcgccgc cttggcgggt ttctcttcgc ccgcgcaccg
      361 gctaggctga tggcacgccc ccgtttgtcc tttggctgga gcaacccatg atccccaacg
      421 cctaccgccg cgtcgtgctg gtgcgtcgtc cccccggcga gccggccgaa gccgatttcc
      481 gcatcgagca ggtccccatg cccgagccgg ggccacgcca gattctggtg cgcaatattt
      541 acatgtccct cgatccctac atgcgcggtc gcatgcgcga ggccaaatcc tacgccgcgc
      601 cggtggccat cggtgaggtc atgacgggtg gcaccgtcgg cgaagtggtg aagtccaatc
      661 accccgatta caaggtgggc gacatcgtcg aggatcgcct gggttggcag gagtacggcg
      721 tcggccccag cgcggcggcg cgtaaggtcg atccgacgct tgccccgatc tcgacggcca
      781 acggtgtgct cggcatgccc ggcatgaccg cctacttcgg cctgtttgac gtcggccgac
      841 cgaaagccgg agagaccgtg gtggtctcgg cggcttccgg cgcggtcggc cagattgtgg
      901 gccaacttgc caagcttgcc ggctgccgcg ccgtcggcat tgccggcggg ccgcaaaaat
      961 gtgccttcgt caaggacgct ctcggttttg atgcctgcat cgactacaag gcgggcaacg
     1021 acctcggcga ggcgctaaag gccgcttgcc ccgatggggt cgacgtctat tttgacaatg
     1081 ttgggggagc cgtgagcgac gccgtcttcc tcaacctcaa cttctgggcg cgcgtggctc
     1141 tatgcggttc gatttcgcaa tacaacgttg ccgcgcctga gctcggcccg cgcctgctcg
     1201 gtctcttcgt cggtcgccgc gtcaacatgc gcggcttcat cgtttgggac ttcaacggcc
     1261 agtacggtcc ggccatgcgc cagatgggtg agtgggtgcg cagcggccgc ctcaagtaca
     1321 aagaggacat cgtcgaaggc ctcgagcagg caccgggcgc cttcatcggc ctgttgcggg
     1381 gcgagaattt cggcaaattg caggtgaagc tcgggccgga cccgacggca cgctaagctt
     1441 tgccggaaaa gttttgccgg aaaagtttgg caacaaaagc ttggtaggaa ggcaccgccg
     1501 ccgg
//
LOCUS       DASTMT010000547         1633 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_647493, whole genome shotgun sequence.
ACCESSION   DASTMT010000547 DASTMT010000000
VERSION     DASTMT010000547.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 1633)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1633)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1633
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_647493"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            161..1321
                     /locus_tag="VFR00_12520"
     CDS             161..1321
                     /locus_tag="VFR00_12520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009491686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEU0160138.1"
                     /translation="MSEAIGNESFPYFGDEHRMLRASIRRFIAERVLPAGDRWEEQGY
                     VPRQILTEMGALGLLGMRYAPEYGGSGLDTLANAVLAEELGRSTYGGFAVTVLVHTDM
                     ASPHLYHAGSKAQLERWMRGITSGRTITAIAMTEPDAGSDLAAMRASARKTQGGYCLN
                     GAKTFITNGVHADLVFVAAKTGEPGRGRHLTMFAVERDTPGFRVARTLKKQGWLSSDT
                     AELVFEDCFVPDANVVGEEGRGFYQLVKNLQNERIVLAAQALGEAMKALELTLDWVKQ
                     RQVFGGPLWDKQTIRQRLSMRCAEVEAARAFLFNTAWRDARNENVVKEVSMLKALAGT
                     LVNEVMYDCLQFHGGMGYMRESAIERMTRDARVQAIGGGATEVMLEEVAKRI"
     gene            complement(1410..>1633)
                     /locus_tag="VFR00_12525"
     CDS             complement(1410..>1633)
                     /locus_tag="VFR00_12525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0160139.1"
                     /translation="PRLTPALLATLLARGFAATARGDFSGHALAQLGAHRDFAGAPRQ
                     DEVAALLASDQFRAALGRIASRYDGGHLD"
BASE COUNT          299 a          523 c          548 g          263 t
ORIGIN      
        1 tcgccgccgc gcacccgcgc tgggcgagca tacggatcag atcctcgccg agctcggcta
       61 caacaacagc gccattgctg ccctccgcga caagggcgtg atctgacgag gcggtgcttt
      121 agcgctgccg gtttttgccg ccaagcggcc aggagcgagc atgagcgaag ccatcggcaa
      181 cgaaagtttt ccctacttcg gcgacgagca caggatgctg cgcgccagca tccgccgctt
      241 catcgccgag cgcgtgctgc ccgccggcga ccgctgggaa gagcagggct acgtgccgcg
      301 tcaaatcctg accgaaatgg gcgcgctcgg gttgctcggc atgcgctacg cccccgagta
      361 cggcggctcc ggccttgata cgttggccaa cgccgtgttg gcggaggaac tcgggcgttc
      421 cacctacggc ggttttgccg tgacggtctt ggtgcatacc gatatggctt caccgcacct
      481 ctaccacgcc ggcagcaagg cccagctcga gcgctggatg cgcggcatca cgtccggccg
      541 caccatcacc gccatcgcca tgacggagcc cgacgcgggg tcggaccttg ccgccatgcg
      601 cgctagcgcc cgcaagacgc aaggcggcta ttgcctcaac ggcgcaaaaa ccttcattac
      661 caatggcgtg catgccgacc tcgtgttcgt cgccgccaag accggcgagc cggggcgcgg
      721 gcgccacctc accatgttcg cggtcgagag agacacgccg ggcttccgcg tggcgcggac
      781 gctcaaaaag caaggctggc tgtcaagcga taccgccgag ctggtgtttg aggactgttt
      841 cgtgccggat gcaaacgtcg tgggtgaaga gggtcgcggc ttctaccaat tggtcaagaa
      901 cttgcaaaat gagcgcatcg tgctggccgc ccaggcgctt ggcgaggcca tgaaggcctt
      961 ggagctcacg ctcgattggg tcaagcagcg ccaggtcttt ggcggcccct tgtgggacaa
     1021 gcagacgatc cgacaacgtc tgtcgatgcg ctgcgcagag gtcgaggcgg cgcgcgcctt
     1081 cttgttcaat accgcttggc gcgacgcgcg caacgaaaac gtggtcaagg aagtatcgat
     1141 gctcaaggcc ctggccggca cgctcgtcaa tgaggtgatg tacgactgcc tgcaatttca
     1201 cggcggcatg ggctacatgc gtgagagcgc gattgagcgc atgacacggg acgcacgcgt
     1261 gcaggcgatt ggcggcggcg ccaccgaggt catgctggag gaggtcgcca aaaggatctg
     1321 aggacggtca tgcccagggc tgcggctggc gcgctttcgc gggaattttc acaccggctt
     1381 gcagccagga tcgccgggcc gcggagcgtt cagtcgagat ggccgccatc gtaacgcgaa
     1441 gcaatgcgcc ccaaggcggc acgaaattgg tcgcttgcca acagcgcggc gacctcgtcc
     1501 tgcctcggcg cgccggcaaa atcgcggtgc gcaccgagct gggccagcgc gtggccgctg
     1561 aaatcgcctc ttgccgtggc agcgaaaccg cgcgcgagca aagtcgcaag cagcgccggc
     1621 gtcaggcggg gca
//
LOCUS       DASTMT010000548         1876 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_1956712, whole genome shotgun sequence.
ACCESSION   DASTMT010000548 DASTMT010000000
VERSION     DASTMT010000548.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 1876)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1876)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1876
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1956712"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..678)
                     /locus_tag="VFR00_12530"
     CDS             complement(<1..678)
                     /locus_tag="VFR00_12530"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEU0160140.1"
                     /translation="MSAAGGPTSMLAAHGERRLLTILFCDLVDSSHLAGRLDPEDLRE
                     VLTSYQRRATEIVEAFGGMVARYQGDGILAYFGYPAASEDDAERAVNAAFELTRRIND
                     GSGVEEVLRVRVGVATGIVVVGDLMRSGAADTPAIVGETPNLAARLQTVAEPDTVLIS
                     RSTQRLTRGLFDYRRLELSGLKGYPTPLEAFQVLGPSEVANRFAALRSTLPLVGRDAE
                     LARLLALW"
     gene            complement(1027..1731)
                     /locus_tag="VFR00_12535"
     CDS             complement(1027..1731)
                     /locus_tag="VFR00_12535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020088027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEU0160141.1"
                     /translation="MSTHRPVAAMLGRTILFKSLAERDRQLVARQMVSATFAPEQLIF
                     ARGDPPDDIYLVLKGRVRLSVFSLDGRMLSFKHAEPGDTFGEIGPLDGGARTADATAL
                     TQVEVMSLERGKLNALLEGNPRVARAAIKFLCARLRETSELAEGIALHSIEMRLARFL
                     LSALKRQRGDGAALHAFIELGITQGELALLIGASRQKVNAALNTLEQAGAIRRTRTRL
                     FCDESSLLRVAALEQG"
BASE COUNT          350 a          607 c          634 g          285 t
ORIGIN      
        1 ccaaagggcc agcaagcgcg cgagctccgc gtcacggcca accaatggca gcgtcgagcg
       61 cagggcggca aagcggttgg ccacttcgct gggcccgagc acctggaacg cctccagcgg
      121 cgtcggatag cccttgagcc ccgatagctc cagccggcgg tagtcgaaca ggccgcgcgt
      181 cagccgttgc gtgctgcgag agatgagaac ggtgtcgggc tcggccaccg tctgcagccg
      241 ggcggcgaga ttgggcgtct cgccgacaat ggcgggcgta tcggcggccc ccgatcgcat
      301 gagatcaccg accaccacga tgccggtcgc aaccccgacg cgcacgcgca aaacctcctc
      361 gaccccgctg ccgtcgttga tgcggcgagt aagctcgaag gcggcattca cggcgcgctc
      421 ggcgtcgtcc tcactggcgg ccggataacc gaagtaggcg aggatgccat ctccctgata
      481 gcgcgcaacc atgccgccga acgcctccac gatttcggtc gcccggcgct gatagctggt
      541 gagtacctcg cgcagatcct ccggatcgag ccgtcccgct aggtgggagg agtcgacgag
      601 atcgcagaat aaaatcgtga gtaaccggcg ctcgccatgc gcggcgagca ttgacgtggg
      661 gccgcccgcc gcgctcatgg ctgcctcgcc gccacggctg tggcaggcgt gcgggcgatt
      721 gcctcggggc ctcggcccga gatgccttgc cgcctgcggc gggggccgtc aggcgccgca
      781 agagatggcg tccgaatggg cagctccttg atcgaagaca cgcgaaaatg cctatggcga
      841 cggcacaagc ccggattctt gtcacgcaag tgacagacct tagcggcggt tgggcgctag
      901 atgaaagcgc gttggtcggc ggcacagggc aatccgggcc gatttgtccg gtgcggggct
      961 gcggccttag gattgcccac ggcgggccac aggtgcgggg tcctgtggcc cgtacctaca
     1021 ccaggcctag ccctgctcga gggcggcgac gcgcagcaga ctgctctcat cacaaaacag
     1081 gcgcgtgcgg gtgcgccgga tcgcacctgc ctgttccagc gtattgagcg ccgcgttgac
     1141 cttttgccgg ctggcgccaa tcaagagggc gagctcgccc tgggtgatgc cgagctcgat
     1201 gaaagcgtgc agcgccgccc cgtcgccgcg ctgccgcttg agcgccgaca gcaggaagcg
     1261 cgcgagccgc atttcgatcg aatgcaaggc gatgccctcg gccagctcgc tggtctcacg
     1321 cagccgggcg cataaaaact tgatggcggc ccgggcgacg cgcgggttgc cctccagcaa
     1381 ggcattgagc ttgccacgtt ccagcgacat cacctccacc tgcgtcaggg cggttgcatc
     1441 ggcggtgcgt gcgcccccgt ctagggggcc gatctcgccg aaggtatcgc cgggctcggc
     1501 gtgcttgaag gagagcatgc ggccgtccaa tgaaaacacc gacaggcgga cgcgcccctt
     1561 gagcacgaga taaatgtcgt cgggtggatc accccgcgca aaaatcaact gctcgggtgc
     1621 aaaggtggcc gaaaccatct gtcgggcgac cagctggcgg tcccgctcgg ccaaggattt
     1681 gaacaggatc gtgcggccga gcatggccgc aacgggacga tgagtgctca tgagggatcg
     1741 aacgtcgcga aaggtcctaa aacaaaaaac aattggcgcg atcttgctca ggcctcaacg
     1801 gcgtgtccag tatccgcgcc caaaaggtcc agcaaaacac gcgcgatgac aatggttgcg
     1861 cggcgtagat ccgcaa
//
LOCUS       DASTMT010000549         7543 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_4754189, whole genome shotgun sequence.
ACCESSION   DASTMT010000549 DASTMT010000000
VERSION     DASTMT010000549.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 7543)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7543)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7543
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4754189"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..651
                     /locus_tag="VFR00_12540"
     CDS             <1..651
                     /locus_tag="VFR00_12540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002715222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0160142.1"
                     /translation="TFTALQEAERLDRAPVAAVLRDHGKEILLSAFARMAEQAPFYIF
                     TAFVFAYAVNTLKVPRDFILIAVLAAAVLSFVSIPLFGHISDRIGRKKTYIAGAVLTG
                     VFGFVYFAMLNTGSYPIIFLAIVLSLIPHDIMYGPQAALIAELFPGRLRYSGASLGYQ
                     LASVIAGGPAPLIATWLFATYGTPYAIAVYILACAVVSVVATALMHDYTGKALKDH"
     gene            complement(701..1489)
                     /locus_tag="VFR00_12545"
     CDS             complement(701..1489)
                     /locus_tag="VFR00_12545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009339785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HEU0160143.1"
                     /translation="MKANALKKKIADGVNCVGGWAAIPNPFAAEIYAAQGWDSVTIDM
                     QHGASDINDVVPILQAIQSGSDATPLVRVPWNDPGHIMRVLDAGAMGIICPMINTRQE
                     AEALVRAGRYPPLGERSFGPFRAAQYGADYWQKANDEILLFAMIETRQAVNNLEEILS
                     VKGVSGVYVGPSDLSLSLGKPPTLDPSDKEVLAAIDTIVKTTRNKGLIAGVHTDGPAT
                     ALRRFEAGFQMCTILNDVRLLAVAAANAINEVRGKAPAAKAKTY"
     gene            complement(1535..2233)
                     /locus_tag="VFR00_12550"
     CDS             complement(1535..2233)
                     /locus_tag="VFR00_12550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008968690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HEU0160144.1"
                     /translation="MIDAALIEKLKSYDTPTICNALEIVDPARRLTGFTMRQLVCPFP
                     NLPPIVGYAKTATIRTTHAHELDAKAQRSQRIAYYEYIADKPAPGIIVIQDLDGHEVG
                     FGAFWGEVMSAVHKGLGLLGVVTDGCIRDIEQWAPGFQALAGSIMPSHAHVHLHDFGK
                     EVRVAGMLVRSGELVHADRHGAVTIPANLADKIPAACDLLARKEAVILEMARAPGFNV
                     AKLREALAKQDEIH"
     gene            complement(2313..3416)
                     /locus_tag="VFR00_12555"
     CDS             complement(2313..3416)
                     /locus_tag="VFR00_12555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015668083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate/lactate/ureidoglycolate dehydrogenase"
                     /protein_id="HEU0160145.1"
                     /translation="MVKIGAAALSEFVREIFAKSGCSAVESERIGKYLVSANLTGHDS
                     HGVARVPRYLQWKNDGVVIADQKVEILSETPVLAVLDGRHGFGQTVGPQAVAIGIEKC
                     KAMGLSLVALRNSGHIGRIGDWAEMAADAGLVSVHFVNASGSVLVAPFGGSERRLSTA
                     PFAVGVPRPGQSPIILDFATSVVAEGKVLVASQGGKALPAEALIDSDGRMSADPRVLY
                     GDYTATGLRQGGKGTGAIRAFGEHKGSGLALMCELLGGSLTGMKATGSEGAAQDRFRG
                     NGMLSFYVDPKVVDPEDIFPGDVARYVAYFKSAKPAVAGGEVLIPGEPEERTRQQRLR
                     DGVPLPDDTWASIIEAARAVGVSERRIQSLSAA"
     gene            3745..4131
                     /locus_tag="VFR00_12560"
     CDS             3745..4131
                     /locus_tag="VFR00_12560"
                     /inference="COORDINATES: protein motif:HMM:NF045268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0160146.1"
                     /translation="MAPKRKTMPRKGAGGRAAHDVGGLNFGKIDRSEHDPALWEKRTD
                     AMQRLLTSPGKRAFSVDGLRRVIEDYGEQAYDRTTYYEKWIRALRNLVVEQELMTRAE
                     LEARMAEVRARHAKAGRRVSKAKIPW"
     gene            4137..4451
                     /locus_tag="VFR00_12565"
     CDS             4137..4451
                     /locus_tag="VFR00_12565"
                     /EC_number="4.2.1.84"
                     /inference="COORDINATES: protein motif:HMM:NF014289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018822 - nitrile hydratase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3-like domain-containing protein"
                     /protein_id="HEU0160147.1"
                     /translation="MSARRLEDSQTPRFAPGDRVRVPSRPVLGHCRTPWYVRGKRGVV
                     SAVHGRFHDPERLAYHFPGLPAQVLYKVRFAQQEMWPSYRGRADDHIEIDIYEHWLEP
                     AR"
     gene            4460..5158
                     /locus_tag="VFR00_12570"
     CDS             4460..5158
                     /locus_tag="VFR00_12570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009734922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrile hydratase subunit alpha"
                     /protein_id="HEU0160148.1"
                     /translation="MHEDDHDHGAEGAAHGGHAHPPLQKDHDAGTMSPYEVLEEALRA
                     LLIEKGILSAEEIAGAVDLMDSRTPALGAKVIARAWVDPAFKKRLLKDTRAALLELDI
                     DIGTIAEFATVENTPEVHNVIVCTLCSCYPKMLLGLPPAWYKSLAYRSRTVVDPRGVL
                     GEFGLELPGEVEVRVHDSTADLRYLVLPMRPKATRGWNEEALAAIVTRDCMIGTALPR
                     VTAKAGPRRAPRAA"
     gene            complement(5296..5625)
                     /gene="trxA"
                     /locus_tag="VFR00_12575"
     CDS             complement(5296..5625)
                     /gene="trxA"
                     /locus_tag="VFR00_12575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018264919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEU0160149.1"
                     /translation="MAGAAREVSDANFEKEVLQSAEPVLVDFFAEWCGPCKAMAPALE
                     QVATELAGKIKVAKLDVDQNPDVTQKYRIQAMPTLMIFKNGEVAAQRVGALVQKKQLT
                     DWINGAI"
     gene            complement(5697..>7543)
                     /locus_tag="VFR00_12580"
     CDS             complement(5697..>7543)
                     /locus_tag="VFR00_12580"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HEU0160150.1"
                     /translation="RLAARIADTIKGWLNGGERLASENRAVRPGDILILVRKRMPFAP
                     AMVSALKARRIAVAGADRLILTEQIAVEDLLALGDVMLLPEDDLALAALLKSPLFDRT
                     DWDLIGLAHGRPASLWQALLRRASEDAHWQPVADRLLAWQAAARQLSPFAFFASVLDR
                     DGGRAHMLARLGSEAADPLDEFLSQALAYEANGAPSLQGFLVALRQARPEIKRDMEQG
                     RDEVRVMTVHGAKGLEAPIVFLPDTCSTRSGRWPGSLLAVGATGAPMAPAFLWPVRGT
                     GTQAAVEAARQAERQADRCELDRLLYVALTRARDRLYVAGFEGTREAPADCWYHLIRQ
                     GVAEHLVEHGVGEGRSVWRLESAQTVAPDKAAHGPGPRVADVPAPAWIARPAPPAEAA
                     ALPLRPSQLVPLEAAGARREPAEARARELPVMGPRALLEEGRFLRGTLTHALFEHLPE
                     VPPEQRQAAAAALLASRAPDLPASLRAQIIAETLTVLTHPELSALFGPGSRAEVPIVA
                     TLRRAPGGAPLRLTGKIDRLVQTATSVLIVDYKTNRPPPRRPAEVAEAYLMQLAAYRL
                     AVAAIFPGLQIRAALLWTDGARIMEIPAALLQEHERRLWTGTLASLDA"
BASE COUNT         1209 a         2543 c         2524 g         1267 t
ORIGIN      
        1 accttcaccg cgctgcaaga ggccgagcgc ctagaccgcg cgccggtggc cgcggtgctg
       61 cgcgaccatg gcaaggagat cctgctcagt gcgttcgccc gcatggcgga gcaggcgccg
      121 ttctacatct ttaccgcctt cgtgttcgcc tacgccgtca acacgctcaa ggtgccgcgt
      181 gactttattc tgatcgccgt gctcgcggcc gcggtgctct cgttcgtctc tattccgctg
      241 ttcgggcaca tctccgatcg gatcgggcgc aagaaaacct acattgccgg cgcagtgctg
      301 acgggcgtgt ttggcttcgt ctatttcgcc atgctcaaca cgggctccta tcccatcatc
      361 ttccttgcca tcgtgctgtc gctgatcccg cacgacatca tgtatgggcc gcaggcggcc
      421 ttgatcgccg agctctttcc cggacggctg cgctatagcg gggcttcgct cggctaccag
      481 ctggcctccg tgatcgccgg cggcccggcg ccgctgatcg ccacctggtt gttcgcaacc
      541 tacggcacgc cgtatgcgat tgcggtctat atcctggcct gtgccgttgt gagcgttgtc
      601 gccacggcgc tcatgcacga ctacaccggc aaggcgctca aagaccatta gcccagggga
      661 agcccccggg ccgagcggcc ggggggcgcg cgcgctgacc ttagtaggtt tttgccttgg
      721 cggcgggcgc cttgccccgc acctcgttga tggcgttggc ggcggctacg gccaacaggc
      781 gcacgtcgtt gaggatggtg cacatctgga accccgcctc gaagcggcgc agcgcggtgg
      841 cagggccgtc cgtgtgcacg ccggcgatca ggcccttgtt gcgcgtggtc ttgacgatgg
      901 tgtcgatggc agcgagcacc tctttgtcgc ttgggtcgag cgtcggcggc ttgcccaggc
      961 tgagcgagag gtcggagggg cccacgtaga ccccgctcac gcccttgacc gagaggatct
     1021 cttccaggtt gttgacggcc tgacgcgtct cgatcatcgc aaacagcaag atctcgtcgt
     1081 tggccttctg ccaatagtcg gcgccgtact gcgccgcccg gaagggcccg aagctgcgct
     1141 cgcccagcgg cgggtagcgg cccgcgcgca ccagggcctc ggcctcttgc ctggtattga
     1201 tcatcggaca gatgatcccc atggcgccgg cgtcgagaac gcgcatgatg tggcccggat
     1261 cgttccaggg cacgcgtacc aggggcgtgg cgtcggagcc gctttgaatg gcctgcagga
     1321 tcggcacgac atcgttgatg tcgctggcgc catgctgcat gtcgatggtc acgctgtccc
     1381 agccttgcgc ggcataaatt tccgccgcga acgggttggg gatggccgcc cagccgccga
     1441 cacagttgac gccgtcggcg atttttttct tcaacgcatt ggctttcatg gttcctcctc
     1501 ggtgccgttg gcgtgcctca tgcagtgaat ttcgtcagtg aatttcgtct tgcttggcga
     1561 gggcctcgcg cagttttgcg acgttgaagc ccggcgcccg cgccatctcc aggatcaccg
     1621 cctccttgcg cgccagcagg tcgcaggcgg cggggatctt gtcggcgaga ttggccggaa
     1681 tcgtcaccgc cccgtggcgg tcggcgtgca cgagctcgcc cgagcgcacc agcatgccgg
     1741 cgacgcgcac ttccttgccg aaatcgtgca ggtgcacatg cgcatggctc ggcatgatgg
     1801 agcccgccag cgcctggaag cccggtgccc attgctcgat atcgcgaatg cagccgtcgg
     1861 tgacgacccc cagcaaaccg agccccttgt gcacggcgct catcacctcg ccccagaagg
     1921 caccgaagcc gacctcgtgg ccgtcgaggt cctggatcac aatgatgccc ggcgcgggct
     1981 tatcggcgat atactcgtag taggcgatgc gttggctgcg ctgcgccttg gcgtcgagct
     2041 catgggcgtg ggtggtgcgg atggtcgccg tcttggcgta gcccacgatc ggtggcaggt
     2101 tgggaaatgg gcacaccagc tgtcgcatcg tgaagcccgt cagccggcga gccggatcga
     2161 cgatctcgag cgcattgcag atcgtcgggg tatcgtagct cttgagcttt tcgatcagcg
     2221 cagcatcaat cacggaacgt ctcctctcga cggtcttggg cgatcttgac ctcggcccac
     2281 cgggctggca aacgcaacga gctcctgttg ggtcaagctg cacttagcga ttggatgcgg
     2341 cgctcgctga ccccgacggc acgcgccgcc tcgataatgg aagcccaggt gtcatccggc
     2401 aatggcacgc cgtcgcgcaa gcgctgctgg cgcgtgcgtt cctcgggctc gccggggatc
     2461 agcacctcgc cgccggcgac ggcgggtttg gcgcttttga aataggccac ataacgcgcc
     2521 acatccccgg ggaagatgtc ctccgggtcg accaccttcg ggtcgacata gaaggagagc
     2581 atgccgttgc cgcggaagcg atcctgggcg gcgccctccg atcccgtggc cttcatgccg
     2641 gtcagcgagc cgcccaaaag ctcgcacatc agcgccaatc ccgagccttt gtgttcgccg
     2701 aaggcgcgaa tggccccggt acccttgccg ccctggcgca ggccggtggc cgtgtagtcg
     2761 ccataaagca cgcgcgggtc ggcgctcatg cggccatcgc tatcgatgag ggcctcggcc
     2821 ggcaacgcct tgccgccctg gctcgccacc aacaccttgc cttcggccac caccgaggtg
     2881 gcaaaatcga gaatgatcgg gctttgcccg ggacgcggca cgccgacggc gaagggcgcg
     2941 gtcgacagac gtcgctcgct accgccgaag ggtgccacca gcacgctgcc ggaagcgttg
     3001 acgaaatgca ccgacaccaa gccggcatcg gccgccattt ccgcccaatc gccgatgcga
     3061 ccgatgtgtc cgctgttgcg cagcgccacc agcgaaagac ccattgcctt gcacttttcg
     3121 atgccgatgg cgaccgcctg gggcccaacc gtctggccga aaccgtgccg gccgtcgagg
     3181 acggcgagga ccggcgtctc gctcaagatc tcaacctttt gatcggcgat gacgacgccg
     3241 tcgttcttcc actgcaagta gcgcggcacc cgcgccacgc catggctgtc gtgcccggtc
     3301 aggttggcgg agacgaggta tttgccgatg cgctcgctct cgacggccga acatccggat
     3361 ttggcgaaga tctcccgcac gaactcgcta agcgcggccg ccccgatttt caccatcgct
     3421 cgaacgttcc tttccctcgg gcacgggcct gcgaccccaa ggcgccgttg caaggcgccc
     3481 tttgaccata aacgaggcgc cccgccaagt catggccgcc gggcggcggg cgcttgcctg
     3541 cgccagtcgc cgtcagggcg aagggtgcac cgccgccagg gcaagctgag ccgtgcatca
     3601 ccgtcaagcc gcccgccgca gggctggtca gcggcccgcg ccgcgggcat catggggcaa
     3661 ccgcgccacc tttccgcggc gagctcgggc gagcgcctct cgggtgcggc ccattgcgtc
     3721 aaccccgaag ggagccgccc gccaatggcg cccaaacgca agacgatgcc ccgcaagggc
     3781 gcgggcggcc gcgccgccca tgacgttgga ggattgaact tcggcaagat cgatcgctcc
     3841 gagcacgatc cggccctgtg ggagaagcgc accgacgcga tgcagcgcct gctcaccagt
     3901 cccggcaagc gggccttcag cgtcgatggg ctgcggcgcg tgatcgagga ttacggcgaa
     3961 caggcctatg atcgcacgac ctactacgaa aaatggatcc gggcgttgcg caatctggtg
     4021 gtcgagcagg agctcatgac gcgcgcggag ctcgaggcgc gcatggccga ggtgcgcgcc
     4081 cgtcatgcca aggctggccg gcgtgtctcc aaggccaaga tcccctggtg aaccgcatga
     4141 gcgcacgccg actagaggac agccaaacgc cgcgcttcgc gcccggtgat cgggtgcggg
     4201 tgccgtcgcg cccggtgctg gggcactgcc gcacgccctg gtatgtgcgc ggcaagcgcg
     4261 gcgtcgttag cgccgtgcac ggccgcttcc acgaccccga acgcctcgcc taccatttcc
     4321 cggggctgcc cgcccaggtg ctctacaagg tgcgctttgc ccaacaagag atgtggccga
     4381 gctaccgggg gcgcgcggat gatcacatcg agatcgatat ctacgagcac tggctcgagc
     4441 ctgcgagata ggagggcaca tgcacgagga tgatcacgac cacggggccg agggcgccgc
     4501 gcatggcggc catgcacacc cgccgctgca aaaggatcat gatgcgggaa ccatgagccc
     4561 ctacgaggtg ctggaagagg ccttgcgcgc gctcttgatc gagaagggca tcctcagcgc
     4621 cgaggagatt gccggggccg tcgatctgat ggacagccgt acgcccgcat tgggggccaa
     4681 ggtcatcgcg cgcgcctggg tcgatcccgc cttcaagaag cgcctgctca aggacacgcg
     4741 cgccgccttg ttggaattgg acatcgacat cggcaccatc gccgagtttg caaccgtgga
     4801 gaatacgccc gaggtgcaca acgtcatcgt ttgcaccttg tgctcgtgct acccgaagat
     4861 gctgctgggc cttccacccg cctggtacaa gagcctggcc taccgctcgc gtacggtggt
     4921 ggatccgcgc ggcgtgctcg gcgaatttgg ccttgagctg cccggcgaag tcgaggtacg
     4981 ggtgcacgat tccaccgccg acctgcgcta tctcgtgctg ccgatgcggc ccaaggcgac
     5041 gcgcggttgg aacgaggagg cgcttgcggc catcgtcacg cgcgactgca tgatcggcac
     5101 ggcgctgccg cgggtaacgg cgaaagcggg cccgcgccgc gccccgcgtg ccgcttaagc
     5161 gacacctgcg agccatcgcg cgacagtcct cgcaaattca cgccttgccg gggtctccgc
     5221 ccgatgaaag gacgcaaaag aggcacccgc gggggtgcct gctttagctt tttgtggggc
     5281 gttgtcggga cgcgctcaga tggcgccgtt gatccagtcg gtcagctgct ttttttgcac
     5341 caacgcaccc acgcgctggg cggcaacttc gccgttcttg aagatcatca gggtcggcat
     5401 cgcctgaatg cgatacttct gtgtcacgtc ggggttttgg tcgacgtcaa gcttggccac
     5461 cttgatctta ccggcgagct cggttgcgac ctgttccagg gcaggcgcca tggctttgca
     5521 gggtccacac cactcggcga agaaatcgac gagcaccggc tctgcggatt gcaacacttc
     5581 cttctcgaaa ttggcgtccg agacctcgcg cgctgcgcca gccatgccgg tattctcctt
     5641 gcgatgaacc cgttgatccg ggggaaaacg acgtccggaa cgtagggagg cccccgtcag
     5701 gcgtcaaggc tcgccagggt gcctgtccaa aggcgccgtt cgtgctcctg caacagcgcc
     5761 gccggtattt ccatgattcg cgcgccatcg gtccaaagca acgcagccct gatctgcagc
     5821 ccggggaaga tggcggccac tgccaggcgg taggccgcca gttgcatcag gtaggcctct
     5881 gcaacctcgg ccgggcgccg cggcggcggc cggttggtct tgtagtcgac gatcaggacc
     5941 gaggtcgcag tttgcaccag ccgatcgatc ttgcccgtca ggcgcagggg agccccgccc
     6001 ggggcgcggc gcagggtggc aacaatcggc acttcggcgc ggctgcccgg cccaaacagg
     6061 gcgctaagct ccgggtgcgt cagaacggtc agcgtttcgg caatgatctg ggcacgcagc
     6121 gatgctggca ggtcgggggc gcggctggcg agcaaggccg ccgcggccgc ctggcgctgc
     6181 tcgggcggca cctcgggcag gtgctcgaag agggcatgcg tgagcgtgcc gcgcaggaaa
     6241 cgtccctcct cgagcaaggc acgcggcccc atcacgggca gctcgcgagc ccgagcctct
     6301 gcgggctcgc gccgggcgcc tgccgcctcg agcggcacga gctgtgaggg gcgcagtggc
     6361 agtgccgccg cctcggcggg cggggcggga cgcgcgatcc aggccggcgc tgggacgtcg
     6421 gcgacccgcg ggccgggccc atgcgcggcc ttgtcggggg caaccgtctg cgcgctctcg
     6481 aggcgccaaa cgctgcggcc ctcgccgacg ccatgctcga cgaggtgttc cgcaacgcct
     6541 tgccggatca ggtggtacca acagtcggcc ggagcctcgc gcgtgccctc gaagcccgcg
     6601 acatacaagc gatcgcgggc gcgcgtgagc gccacgtaca gcagacgatc gagctcgcat
     6661 ctgtcggcct gccgttccgc ttgccgcgcc gcctcgaccg ccgcctgcgt gccggtgccg
     6721 cgcaccggcc agagaaaagc cggcgccata ggagcgcccg tcgcgccgac ggcgagcagg
     6781 ctgcccggcc agcgtcccga gcgcgtcgag caggtatccg gcagaaacac gatcggagct
     6841 tccagcccct tggcaccgtg cacggtcatg acgcgaactt catcgcggcc ctgctccatg
     6901 tcgcgtttga tttcgggtcg cgcctgccgg agcgcgacga ggaagccttg cagggatggc
     6961 gccccgtttg cctcataggc gagggcctgg ctcaagaatt catcgagcgg gtcggcggcc
     7021 tcgctgccca aacgcgcgag catatgcgcg cgtccgccgt cgcgatcgag cacgctcgca
     7081 aagaaggcaa aaggcgagag ttgccgcgcc gcggcctgcc aggccagcag gcgatcggcc
     7141 accggttgcc aatgcgcgtc ttcgctggcg cgccgcagca gcgcctgcca taggctcgcc
     7201 ggccggccgt gcgcgaggcc gatcagatcc caatcggtgc gatcgaacag gggagatttc
     7261 aagagcgccg cgagtgccaa gtcgtcctcg ggcaggagca tgacatcgcc cagcgcgagc
     7321 aggtcttcga cggcgatctg ctccgtcaag atcaggcgat ccgcgccggc gacggcaatg
     7381 cgccgggcct tgagggcgga taccatggcg ggcgcgaagg gcatgcgttt gcgcaccaag
     7441 atcaagatgt cgcccggccg cacggcgcgg ttctccgagg cgaggcgctc gccgccattg
     7501 agccagccct tgatggtgtc ggcgatgcgc gccgccaggc ggg
//
LOCUS       DASTMT010000550         1846 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_1628722, whole genome shotgun sequence.
ACCESSION   DASTMT010000550 DASTMT010000000
VERSION     DASTMT010000550.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 1846)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1846)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1846
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1628722"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..732)
                     /locus_tag="VFR00_12585"
     CDS             complement(<1..732)
                     /locus_tag="VFR00_12585"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU0160151.1"
                     /translation="MNLDAPLSPDLSSESEADRRAPAKSRLKIIDGDVHPALRSPADL
                     KPYLSQRWWEHFQTWGGRRRSGMSYEPYPKSQPRASRRDAWPQEGGAPGSSLRLMREQ
                     FLDAYGIECGLLGPLGLTGQSEFNLDFAGALASAVNDWQRESFSKPEPRLKSAIVVPY
                     DDAEAAAREIDKRADDPAYAQVFMLTRSQEPAGRKRYWPIYAAAERHGFPVGMHVFGA
                     SGHPYTGAGWPSYYIEESSGHSTSCQ"
     gene            complement(729..1766)
                     /locus_tag="VFR00_12590"
     CDS             complement(729..1766)
                     /locus_tag="VFR00_12590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008973082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU0160152.1"
                     /translation="MDCDVHPRLPTPKALSAHMDEFWRDTVEARGIDVWDSMAYPANA
                     PFTVRPDWRQGRADATATGLAAATLDRFGFGHSILNCLFPVQTFRDENLGAAFARATN
                     DWIAREWLDKDPRLRASLVLPIQSAARSVEEIERLAGDRRFVQVLMLAMGEAPLGKSR
                     YWPIYAAAERHGFTLGVHAGSSYYQAVTGSGWPSYFIEDYAAQAAGFHTQLGSLICEG
                     VFVKYPKLKVVLIESGVSWLPPYLWRLAKSWRGVRHEVPWLDRSPEEVARDHVRLTLQ
                     PFDAPPGAAHIGKVLDQLQSDDMLLFATDFPHWQFDGDAMLPAGLGPALKRKILIDNA
                     YATYPRLGRSP"
BASE COUNT          316 a          586 c          644 g          300 t
ORIGIN      
        1 ttggcaggag gtggaatggc ccgagctttc ctcgatgtag taggacggcc aaccggcgcc
       61 ggtgtaggga tggccgctgg cgccgaacac gtgcatgccg acggggaagc catgccgttc
      121 ggccgccgca tagatcggcc agtagcgctt gcggccggcg ggctcctggc tgcgcgtcaa
      181 catgaacacc tgggcgtagg ctggatcatc ggcacgcttg tcgatctcgc gcgcggccgc
      241 ctcggcgtcg tcgtagggca cgacgatcgc cgacttgagg cgcggctccg gtttggaaaa
      301 gctctcgcgc tgccagtcgt tgaccgccga ggcgagggcg ccggcaaaat ccagattgaa
      361 ctcgctctgt cccgtcaggc cgagcgggcc caacaggccg cactcgatgc cgtaagcgtc
      421 gaggaactgc tcgcgcatca ggcgcaggct gctgcccggg gcaccgccct cttgcggcca
      481 ggcatcgcgc cggctggccc ggggttgcga ctttggatag ggctcatagc tcatgccgct
      541 gcggcggcgg ccaccccaag tctggaaatg ctcccaccag cgttgcgaca ggtagggctt
      601 caagtcggcg ggtgagcgca gggcgggatg cacatcgcca tcgatgatct tgaggcggct
      661 ttttgccggc gcgcgccgat cggcctcgct ctcgctggac aaatcggggc tcagaggggc
      721 gtcgaggttc atggggacct cccgagacgg ggataggtgg cataagcgtt gtcgatcagg
      781 atcttgcgtt taagcgccgg ccccaggccg gcgggcagca tggcatcgcc gtcgaactgc
      841 cagtgcggaa aatcggtggc aaacagcagc atgtcatcgg actggagctg atccaaaacc
      901 ttgccaatgt gcgctgcgcc gggcggcgcg tcaaagggtt gcagggtgag gcgcacatga
      961 tcgcgcgcga cctcctcggg cgagcggtcg agccagggca cttcgtggcg cacgccacgc
     1021 caggacttgg ccaaccgcca caggtatggg ggtaaccagg aaacgcccga ttcgatcaat
     1081 acaaccttga gcttggggta tttcacgaac accccctcgc agatcaggct gccgagctgg
     1141 gtgtgaaaac ccgcggcctg ggcggcatag tcttcgataa agtagctggg ccaacccgag
     1201 ccggtcacgg cctggtagta gctgctgccg gcatggacgc cgagggtaaa gccgtgcctt
     1261 tcggccgcgg catagatcgg ccagtaacgg gatttcccga gcggcgcttc gcccatcgcc
     1321 aacatcagga cctgcacgaa gcgccggtcg ccggccaggc gctcgatctc ctccaccgac
     1381 cgggccgctg actggatcgg cagcaccagg gaggccctga ggcggggatc cttgtccagc
     1441 cactcgcgcg cgatccaatc gttggtcgcc ctggcaaagg cggcgccgag gttctcatcg
     1501 cggaaggtct gcaccggaaa caggcaattg aggatgctgt ggccgaagcc gaaacggtcg
     1561 agggtggcgg cggccaagcc ggtggcggtg gcatcggcgc gcccctggcg ccagtccggc
     1621 cgcaccgtga agggggcgtt ggccggatag gccatggaat cccagacatc gatgccgcgc
     1681 gcttccacgg tgtcgcgcca gaattcgtcc atgtgcgcgc tcagcgcttt gggcgtcggc
     1741 aggcggggat ggacgtcgca gtcgatcgcg gcccgaaact cgccccgaaa ctcgccctgg
     1801 acctcgccct gagactggct cgtcacgcgc gctcccttgg atctgt
//
LOCUS       DASTMT010000551         5994 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_2290216, whole genome shotgun sequence.
ACCESSION   DASTMT010000551 DASTMT010000000
VERSION     DASTMT010000551.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 5994)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5994)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5994
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2290216"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..561)
                     /locus_tag="VFR00_12595"
     CDS             complement(<1..561)
                     /locus_tag="VFR00_12595"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU0160153.1"
                     /translation="MTQELQGAIDCDLHPRLPTPKALSAHMDEFWRDTVEVRGIDVWD
                     SMAYPANAPFTVRPDWRQARADANAASLAAATLDRFAFKHGILNCLFPVQTFRDENLG
                     AAFARATNDWIRREWLDKDPRLRASLVLPIQSPARSVEEIERLAGDPRFVQVLALAMG
                     ETPLGKPRYWPIYAAAEKHGFALGIHA"
     gene            848..1945
                     /locus_tag="VFR00_12600"
     CDS             848..1945
                     /locus_tag="VFR00_12600"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012562164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03617 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HEU0160154.1"
                     /translation="MRLETLLPLGKVDPGLRAPETGLDLAAVAADAALLEEIGYDGVA
                     VEETKQDPYIVMALAAAATRRLRVSTAIAMAFPRSPTVTALSAWTLQKLAKGRFTLGL
                     GPQVRAHIERRYGLAWSAPGPWMREYVAAVRAVWRCWQDNQPLDVKGPHYKLNLMPPL
                     FNPGPIEHPAIPIHLAAVNVGMCQVAGEVADGVRPHPICSAQYIAEVMIPELRKGAAR
                     AGRSLAEFKICHKPLVATARNTDELELRVRDVRARLAFYISTPSYRPAVEHHGLGDLA
                     RKLSLFARAQDWEKMPPCVSDEVLQLFAVVGTHEEIAAKLLERFGHVVTHCEFSIAVR
                     NEADKEQLTRIAATLKAAPDTAVRRRLMSPG"
     gene            complement(1969..4359)
                     /locus_tag="VFR00_12605"
     CDS             complement(1969..4359)
                     /locus_tag="VFR00_12605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019198547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEU0160155.1"
                     /translation="MSDSIDPGSGDRPNSYIGKSVPRPNAAKLVEGRGQYVDDILLPR
                     LLHVAFLRSPHAHAKILNIDAAPALELPGVRRAFTGRELAEHCDPWVAVLAHFKGMKS
                     PPQLPLPLERTTWAGEAVAAVVADSRTLAEDACAKIAVSYQPLAAAVDMESALAASAP
                     VAHPELGDNLCFQRLNEAGKVEEAFAGAHRVVEAVFHSGRHTGATLEPRSILADYDRA
                     QGKLTVYHSTQAPHMMQGVFAKHLRLPEGDVRVICTDVGGSYGIKVHVYPDEVAVAVI
                     AKIMARPIKFIADRLESFTSDIHARDHRIKARMAVDATGAILAIDIDDLTGIGPYSVY
                     PRTSAVEGNQVVNLVGGPYDFANFRAKTTVVFQNKTPTCQYRAVGHPIATAVTEGLVD
                     LAAEALDLDPAEFRRRNLMRDNSYPRSSPAGMKFEALSHEQSLATLLAMMDYATLRAE
                     QARLREKGIYRGIGLASFIELTNPSPFMYGVGGARISAQDGCWVRIDPDGSVLAATGV
                     TEQGQGTEAIIRQIVAEAVGVPIDRVRVMTGDTLTTPYGGGTWACRGAGIGGEAALQA
                     GLAVKQAALRVAAAMLQAQPDSLELSNGEIIDKASRQARMPLAEVSRIVYFRGDTLPP
                     DLPRELMQTRHFITKQYPFAFTNGVQASWLEVDTDTGMIRLIKHWCVEDCGRIINPLL
                     VDEQVRGGIVQGIGGALYEHCLYSPSGQLLNASMADYLVPMAAEMPDIEVGHVETPTK
                     ESLLGAKGAGEAGTAGAPAAVMNAINDALKPFGAKVFAQPFTPERILAALGKVKAR"
     gene            complement(4365..4853)
                     /locus_tag="VFR00_12610"
     CDS             complement(4365..4853)
                     /locus_tag="VFR00_12610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012330530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEU0160156.1"
                     /translation="MSQVVELSLTVNGERVTRRIEARQHLIDFLRLELGLTGSHTGCE
                     HGVCGACTVRVDGRIVRGCLVLAACLEGATVETIEGLSETGEIRDLQDAFIRRNAAQC
                     GYCTPGMLLTAAELLRVNPRPAREEIREYLAGNYCRCTGYHAIVDAVEATAAARRGPR
                     QA"
     gene            complement(4850..5725)
                     /locus_tag="VFR00_12615"
     CDS             complement(4850..5725)
                     /locus_tag="VFR00_12615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020094442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HEU0160157.1"
                     /translation="MKAPPFEYVRAKALADVFQLWAEAGPEARLLAGGQSLIATLALR
                     LSEPSTLIDITRVPELRGIELSGDTLRLGALTTHAELAADALVRQHAPLLAEAAPLIA
                     HPAIRNRGTLGGSLAFADPAAELPACAVALGATIVAVSAAGERRIPARTFFQGLYATA
                     LATNELIVAVEVPLAKAGERSTILELARRSGDYAMAGIVARARLDTGRLADPQIAFFA
                     LGDGPVMARGAMAALADRVPDRAAIAAAAAALAGDLDPPADLNGGSDMKRHLACVLLA
                     RAIERLARGKEARAA"
BASE COUNT          935 a         1996 c         2083 g          980 t
ORIGIN      
        1 ggcatggatg cccagggcaa agccatgctt ttcggcggcc gcatagatcg gccagtagcg
       61 cggtttgccg agcggggttt cgcccatcgc caacgccagg acctgcacga agcggggatc
      121 gccggccagc cgctcgatct cctccaccga gcgggccggt gattggatcg gcagcacgag
      181 ggaggccctg aggcgcggat ccttgtccag ccattcgcgc cggatccaat cgttggtggc
      241 ccgcgcaaag gcggcgccga ggttctcgtc gcgaaaggtc tggacgggaa agaggcagtt
      301 gagaatgcca tgcttgaagg cgaagcgatc gagcgtggcc gcggccaggc tggccgcatt
      361 ggcatcggcc cgcgcctggc gccagtccgg gcggacggtg aagggggcgt tcgccggata
      421 ggccatggag tcccagacgt cgatgccacg cacctccacc gtgtcgcgcc agaactcgtc
      481 catatgggcg ctgagcgcct tgggggtggg caggcgcgga tgcagatcgc agtcgatggc
      541 gccttgcagc tcttgcgtca cggttctcga acctcttgcg gacacgtgtt gcggccgcgc
      601 cgctcgttgg caccggagca tagccgaaag ccgagcgctg gcccaggcgc gccgaggtgg
      661 ctctgtccgc agcggtcacg ccggtgtcgc cggcgcgctg cgcctggccg gggcgcggca
      721 aagagcgaag gaccgccagc gctttacagg ccggcaagcc ctctggcaca aggccggcgc
      781 caacaggcga tcggcgcgct gtagcgccgg cgccctgggg ccgtggtcgc gccaggaggg
      841 gacccgcatg cgccttgaga ccctgctgcc gctcgggaag gtcgatcccg gcttgcgcgc
      901 gccggaaacc ggcctcgatc ttgccgccgt tgccgccgat gccgccttgc tcgaagagat
      961 cggctacgac ggggtcgccg tcgaggagac caagcaagat ccctatatcg tcatggcgct
     1021 ggcggccgcg gcgaccaggc gtctgcgggt ctccaccgcc atcgccatgg cttttccccg
     1081 cagtccgacc gttacggcgc tgagcgcctg gaccttgcag aagctcgcca aagggcgctt
     1141 taccctaggg ctcggcccgc aggtgcgcgc ccatatcgag cgccgctacg gccttgcctg
     1201 gtcggcaccg gggccgtgga tgcgcgagta cgttgccgcc gtgcgcgccg tgtggcgctg
     1261 ctggcaagac aatcagccgc tcgacgtcaa gggcccgcac tacaaactga acttgatgcc
     1321 gccgctgttc aatccggggc ccattgagca tcctgccatt ccgatccatc tcgccgccgt
     1381 caacgtcggc atgtgtcagg tcgccggcga ggttgccgac ggcgtgcgcc cgcatcccat
     1441 ctgctcggcg caatatatcg ccgaggtcat gatccccgag ctgcgcaagg gtgccgcgcg
     1501 cgccggccgc tcgcttgccg agttcaagat ctgtcacaag ccgctggttg ccacggcgcg
     1561 caacaccgac gagctcgagc tgcgcgtccg cgacgtccgc gcgcgcttgg cgttttatat
     1621 ttcgacgccg tcctaccgcc cggcggtcga gcaccacggc ctgggcgatc tggcgcgcaa
     1681 actttccctg ttcgcccgcg cccaggactg ggagaaaatg cctccctgcg tcagcgatga
     1741 ggttttgcag ctcttcgccg tggtcggcac gcacgaggag atcgccgcca agctgctcga
     1801 gcgcttcggc catgtcgtca cgcactgcga attttccatc gcggtccgca atgaggccga
     1861 caaggaacag ctgacccgga ttgccgcgac cctcaaggcc gcccccgaca cggcggtgcg
     1921 gcggcggctc atgagccccg gctaaggctg ggtgagaggg gggtgcggtt aacgcgcctt
     1981 gaccttgccc agggctgcca agatgcgctc cggcgtgaag ggctgggcga acaccttggc
     2041 gccgaagggt ttcagggcat cgttgatggc attcatcacc gccgccgggg ccccagcggt
     2101 cccggcttcg cccgcgccct tggcgcccaa cagggactcc ttggtcgggg tctcgacgtg
     2161 gccgacctcg atgtcgggca tctccgcggc catcggtacc aaatagtcgg ccatcgaagc
     2221 gttgaggagc tggccgctcg gagaatagag gcagtgttcg tagagggcgc cgccgatacc
     2281 ctgcacgatg ccgccgcgca cctgttcgtc caccaacagg ggattgatga tgcggccgca
     2341 gtcttccacg caccaatgct tgatcaacct gatcataccg gtgtcggtgt cgacttcgag
     2401 ccagctcgcc tgaacgccgt tggtgaaggc aaagggatac tgtttggtga tgaagtgtcg
     2461 cgtctgcatg agctcgcgcg gcagatcggg cggcagtgta tcgccacgga agtagacaat
     2521 gcgggagacc tcggcgagcg gcatgcgcgc ctgccggctc gccttgtcga tgatttcgcc
     2581 gttgctgagc tccaagctgt cgggctgcgc ctgcagcatg gccgccgcca cgcggagcgc
     2641 cgcctgcttg acggccagcc ccgcctgcag ggccgcctcc ccgccgatgc cggcgccgcg
     2701 gcaggcccag gtgcccccgc cgtagggcgt ggtcagcgtg tcgccggtca tgacgcgcac
     2761 gcgatcgatc ggcaccccca ccgcctcggc cacgatctgg cggatgatgg cttcggtgcc
     2821 ctgaccctgc tcggtcacgc ccgtggctgc cagcaccgag ccgtctggat caatgcgcac
     2881 ccaacagccg tcctgggcgg agatgcgcgc gccgccgacg ccgtacatga aggggctggg
     2941 gttggtgagc tcgatgaagc tcgccaggcc aatgccgcga tagatgccct tttcccgcag
     3001 gcgcgcctgc tcggcgcgca gcgtggcata gtccatcatc gccagcaggg ttgcgagcga
     3061 ctgctcatgg ctcaaggctt cgaacttcat gccggccggt gagctgcgcg gatagctgtt
     3121 gtcccgcatc aggttgcggc gccgaaactc cgccggatcg aggtccagcg cctccgcggc
     3181 caaatccacg aggccctcgg tgaccgccgt cgcgattgga tgcccgaccg cgcgatactg
     3241 gcaggtcggc gtcttgttct ggaacaccac cgtggttttg gcgcggaagt tggcaaagtc
     3301 gtaggggccg ccgacgaggt tgacgacctg gttgccctcc accgcgctgg tacgcgggta
     3361 aaccgaatag gggccgatgc cggtcagatc gtcgatatcg atggccaaaa ttgcgcccgt
     3421 ggcatcgacg gccatgcgcg ccttgatgcg atgatcgcgc gcatgaatgt cgctcgtgaa
     3481 gctttccaac cggtcggcga tgaatttgat cggccgggcc atgatcttgg cgatcacggc
     3541 gacggcaacc tcgtcgggat aaacgtgcac cttgatgccg tagctgcccc cgacgtcggt
     3601 gcagatcacc cgcacatcgc cctcgggcag ccgcagatgc ttggcgaata cgccctgcat
     3661 catgtgcggt gcctgcgtgg agtgataaac cgtgagcttg ccctgcgccc gatcataatc
     3721 ggccaggatc gagcgcggct cgagcgtggc gcccgtgtgc cggccgctgt ggaagacggc
     3781 ctccaccacg cggtgcgcgc cggcgaaggc ctcctcgacc ttgccggctt cgttcaggcg
     3841 ctggaagcag agattgtcgc cgagctcggg atgggcgacc ggagccgatg cagcgagcgc
     3901 gctctccatg tccaccgccg cggcgagcgg ctggtagctg acggcaatct tggcacaggc
     3961 gtcttcggcg agcgtgcggc tgtccgccac cacggcggcg acggcttcgc ccgcccaggt
     4021 cgtgcgctcg agcggcaagg gcagctgcgg cggcgacttc atgcccttga aatgggcgag
     4081 caccgcgacc catgggtcgc aatgctcggc gagctcgcga cccgtgaagg cccgacgcac
     4141 acccggcagc tctagggccg gagccgcatc gatgttgaga attttggcgt gtgcgtgcgg
     4201 gcttctgagg aaggcgacgt ggagcaggcg cggcagcagg atgtcgtcga cgtattgccc
     4261 gcggccttcg accaatttgg cggcgttcgg gcgcggcacc gacttgccga tataggaatt
     4321 aggacggtcg ccgctaccgg gatcgatgct gtcgctcatg ggcctcatgc ctgtcggggg
     4381 ccacgccgcg ccgccgccgt ggcttccacg gcatcgacga tggcgtgata gccggtgcag
     4441 cggcagtagt tgccagccag gtattcgcgg atctcctcgc gcgccgggcg tggattgacg
     4501 cgcaacagct cggcggccgt cagcagcatg cccggcgtac agtacccgca ctgggccgca
     4561 ttgcgccgga tgaaggcgtc ctgcaaatcc cggatctcgc ccgtctcgga gaggccctcg
     4621 atggtttcga ccgtggcccc ctcgaggcag gccgccagca ccaggcagcc gcgcacgatg
     4681 cggccgtcga cgcgcaccgt gcaggcgccg cacacaccgt gctcgcagcc ggtgtgtgag
     4741 cccgtcaaac cgagttcaag gcgcagaaaa tcgatgagat gctggcgcgc ttcgatgcga
     4801 cgggtcacgc gctcgccgtt gacggtcagg gacagctcca ccacttggct catgcggccc
     4861 gggcctcctt gccccgcgcc aacctctcga tggcgcgcgc cagcagcacg caggcgaggt
     4921 ggcgtttcat gtctgagccg ccgttgaggt cggcgggcgg atcgaggtcg cctgcgagtg
     4981 cggccgcggc tgccgcgatc gctgcccgat cggggacgcg gtcggccaag gcggccatgg
     5041 caccacgggc catgaccggg ccgtcgccga gggcaaaaaa agcgatttgt ggatcggcca
     5101 accggcccgt gtcgaggcgg gcgcgcgcga cgatgcccgc catggcgtag tcaccggagc
     5161 ggcgcgccag ttccaggatt gttgagcgct cgccggcctt tgccagcggc acttcgacgg
     5221 cgacgatcag ttcgtttgtc gccagtgcgg tcgcgtagag cccctggaag aaggtgcgcg
     5281 ccgggatgcg gcgctcgccc gcggcgctca cagccacgat cgtcgccccg agggcaaccg
     5341 cgcaggcggg cagctccgcc gccggatcgg cgaaggccag cgagcctcca agtgtgccgc
     5401 gattgcggat ggccgggtgc gcgatcagcg gcgccgcctc ggccagcagc ggggcgtgct
     5461 gccgcaccag ggcatcggcg gcaagctcgg cgtgcgtggt cagggcgccg aggcgcagcg
     5521 tgtcgcctga aagctcaatc ccccgcaact cgggcacgcg cgtaatatcg atcagtgtgc
     5581 taggctcgga gagccgcagc gccagggtgg cgatgaggct ctggccgccc gccagcaacc
     5641 gggcctcggg cccggcttcg gcccaaagct ggaacacgtc agcgagcgct ttcgcccgca
     5701 catactcgaa tggcggcgcc ttcatggctc aagtccaacg ttgcctgcgc atcactcccc
     5761 ctgcggcggc cggcgcatct tcgcggattg ggttgcgtcg tccctttcgc aaatatgcgg
     5821 cgttaggcca ggtcacgaca agtctcggga tgccccggcg gctcggatgc gcgagggttt
     5881 ggcgcgccgc gtcgggcgaa ggcaggtcag ggcggtctca tcacggactt gaaaggtcta
     5941 ggttttttca ggagtgcggt tacagggggt gagaaaagtc ggcgaggatg tcgg
//
LOCUS       DASTMT010000552         2086 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_977056, whole genome shotgun sequence.
ACCESSION   DASTMT010000552 DASTMT010000000
VERSION     DASTMT010000552.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2086
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_977056"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            62..211
                     /locus_tag="VFR00_12620"
     CDS             62..211
                     /locus_tag="VFR00_12620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0160158.1"
                     /translation="MRYWTSQIVIGIIVTVVGTVIANAVIKAFGGRRHVVDAPHYSGP
                     ARAGR"
     gene            complement(208..1272)
                     /locus_tag="VFR00_12625"
     CDS             complement(208..1272)
                     /locus_tag="VFR00_12625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018643399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU0160159.1"
                     /translation="MSTAAQTSAKAPDYYLPVRPDWLDRRREPIIDADQAIVDPHHHL
                     WDRQDWRYLLDELVADTNSGHNIVATVFVQARAMLRASGPVEMKHVGETEFVNGAAAM
                     SASGVYGKTRHCAGIVGHADLSLGGRVEPVLEAHLRAGGDRFRGVRHSSAWDDDAKVR
                     NPAYNPPPGLLGHSSFREGFAVLGRLGLSYDAWLYHPQIDELADLARAFPASKIVLDH
                     VGGPIGIRAYAGKRDEVFVKWKASIEALARCANVYVKLGGLGMRLGGFDFHTHAEPPS
                     SETLATAWRPYFDTCIEAFGPSRAMFESNFPVDKGSYSYPVFWNACKLIAKGASAAEK
                     ADLFAGTAARFYRLDLDGRG"
     gene            1381..1728
                     /locus_tag="VFR00_12630"
     CDS             1381..1728
                     /locus_tag="VFR00_12630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0160160.1"
                     /translation="MPIKSKYVFMASMDVAADKEALFNEVYDEEHVPNLLKVPGVHAV
                     TRMAGEPFAMSIGGEQKTLSHDGPRYTAIYEIDGPHVLISPDWAKAVEAGRWPAQVRP
                     HTRNRRHALYRVR"
BASE COUNT          389 a          657 c          693 g          347 t
ORIGIN      
        1 gaccgcaatt ttgccctcag cgccccctac aagcgcgcca ggaaataagg cacagggtgc
       61 catgcgatat tggacctcgc agatcgtgat cggcatcatc gtcaccgtgg tcggtacggt
      121 gattgccaat gccgtgatca aggcgtttgg cggccgcagg cacgtggtcg atgccccgca
      181 ctattccgga ccggccaggg ccgggcgcta gccgcggccg tccaggtcca gtcggtagaa
      241 gcgcgccgcc gtaccggcaa acagatccgc cttctcagcg gcactcgcgc ctttggcgat
      301 tagcttgcac gcgttccaga acaccgggta gctgtaggac cccttgtcga cgggaaaatt
      361 actctcaaac atcgcccgcg atggcccgaa cgcctcgatg caggtgtcga aatagggccg
      421 ccaggccgtg gccaacgttt cggaggaggg cggctcggcg tgggtgtgaa aatcgaagcc
      481 gccaaggcgc atgccaagcc caccgagctt gacgtagacg ttggcacagc gggccaacgc
      541 ttcaatcgag gccttccatt tgacgaagac ctcgtcccgc ttgccggcat aggcgcggat
      601 gccgatcggc ccaccgacgt gatcgagcac gatcttgctg gcgggaaacg cccgtgcgag
      661 gtcggccagt tcatcgattt gcgggtgata gagccaggca tcgtagctga ggccgagccg
      721 gcccaagacg gcgaaacctt cgcgaaaact gctatgcccg agcaggcctg gcggaggatt
      781 ataggccggg ttgcgcacct tggcatcgtc gtcccaggcg ctgctgtggc ggacgccgcg
      841 gaagcggtcg ccaccggccc gcagatgcgc ctcgagcaca ggctcgaccc ggccaccgag
      901 gctcaggtcg gcgtgaccga cgataccggc gcaatgccgt gttttgccgt aaacaccgct
      961 ggcgctcatg gcggcggcgc cgttgacgaa ttctgtctcg cccacgtgct tcatctcgac
     1021 aggaccgctg gcgcgcagca tggcgcgggc ctgaacgaac acggtggcga ctatgttgtg
     1081 gccgctgttt gtatcggcga ccaactcgtc gagcagatag cgccaatcct gccggtccca
     1141 aaggtggtgg tgcggatcga cgatcgcctg atcggcatcg ataatcggtt ctcgacgacg
     1201 atcaagccaa tccgggcgca ccggcaaata gtagtccgga gcctttgcgc tggtttgggc
     1261 ggcggtggac atttacggca cactccttaa ggattgacgc gccgccggat cgccggcctc
     1321 ttggccttgc cgccaggagc cgaaacggcg atgaaaccag aagcaagcga gcgagctatc
     1381 atgccaatca agtccaagta cgtgttcatg gcaagcatgg acgtggctgc cgataaagag
     1441 gccttgttca atgaggtcta cgacgaggag catgtgccca acctgctcaa ggtgcccggc
     1501 gtacatgccg tgacgcgcat ggccggagag ccctttgcca tgagcatcgg cggcgaacag
     1561 aagacgctga gccatgacgg cccgcgctat acggccattt atgagattga tggcccgcac
     1621 gtgctcatca gtccggactg ggcgaaggca gtcgaagccg gtcgctggcc ggcgcaggtg
     1681 cggccgcata cgcgcaaccg gcgccacgcg ctttaccgcg tgcgctaagc ctgcttcccg
     1741 ccaccaccgc ccaggctgcc aaggcatgcc ccggcgctgc ggcgttggcg cgcaacttcg
     1801 aggtcgcgga aaccgccggc tttcggggtc aagctttcgg ggccaaggct tcggtgcaag
     1861 agccgagacg cggcggctgc gggtcgggcc tgcttgcccg tcggcgcgcc gccgccaagc
     1921 cgaagccatt cctggaggaa ggggccgcga ggcgacagcg gcgcaccgcg cgggctttgg
     1981 cggcgcggac attctaagct tggacggcgc tgtttttttg ccgcggcggc gaaggtgcac
     2041 gccacaaaag aatgacccgc ggcttggggg aaccgcgggt caatgc
//
LOCUS       DASTMT010000553         2573 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_4252795, whole genome shotgun sequence.
ACCESSION   DASTMT010000553 DASTMT010000000
VERSION     DASTMT010000553.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2573
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4252795"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..792
                     /locus_tag="VFR00_12635"
     CDS             <1..792
                     /locus_tag="VFR00_12635"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEU0160161.1"
                     /translation="GYVMEGRQAGSAMFTKLCAGCHSIGGGDHVGPDLDGLTLRRSRA
                     WISEFLIDPIKMRARQDPIALALAAKFPGVRMPYLQIHASDAADLMSYIDAKSKQQQP
                     TIALESLYALTTQDGGHLRAADLKGQPFAVAFGYTHCPDVCPTTLLEWSNLMRAAGAD
                     LGAFKLLFVSVDEERDTPSALKAMLASFDPRITALTGSAAEIAAVARQFGAFYEKVKG
                     ADGEYSIDHTVKSYLVDQEAHVAGSIDPQTPEPDQQRVLTQLLVR"
     gene            861..1502
                     /locus_tag="VFR00_12640"
     CDS             861..1502
                     /locus_tag="VFR00_12640"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006613137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HEU0160162.1"
                     /translation="MSWPFSDAFRRQVAGRCAAFSRVAEPDGSAGLKRAAVAITLLPL
                     LEAGGPPRQAAFLLTRRAEGLRGHGGQWALPGGRCEAHESSAEAALRELAEEVGVSLQ
                     PDAVLGLLDDYPTRSGYLITPVVLWAGPGQVPRADPNEVASIHRIGLSELTRQDVADF
                     VSIPESDRRVIRLRINGGYVHAPTAAVLYQFGELLQGRTTRVADLEQPVFAWR"
     gene            complement(1515..>2573)
                     /locus_tag="VFR00_12645"
     CDS             complement(1515..>2573)
                     /locus_tag="VFR00_12645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006339946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEU0160163.1"
                     /translation="EVGMKGVVFLGDRKLELRDFPDPTPGPRDVVIEIKASGMCGSDL
                     HVYRAAFKPGDTQSGFKRGSEPVIAGHEPCGVVVALGTGVAPHEAKIGQRVMDHHYKG
                     CGSCKHCRTGWTQMCLDGAVVFGASGNGGHAKYMLAPVTTLVPLPDALSFETGAAISC
                     GTGTAYGALKRSNLQGGETIAIFGQGPVGLSATQLAVAMGARVIALDISAERRQLARE
                     FGAHEVIDARSNDPVAAIRDLTHGEGAHKTLDTSGAAEARAAAVRATRAWGTACYVGE
                     RGQVTLDVSPDLLRRQITLVGSWTFSTQGQADCAEFVADHNIAVEKLFTHRWRLDQAE
                     AAYRLFDTQTTGKGVILP"
BASE COUNT          409 a          891 c          845 g          428 t
ORIGIN      
        1 ggctatgtga tggagggccg gcaggccggc tcggcgatgt tcaccaagct ctgcgccggc
       61 tgtcactcaa tcggtggcgg tgaccacgtc ggccccgatc tcgatggcct caccttgcgc
      121 cgcagccgcg cctggatcag cgagtttctc atcgacccca tcaaaatgcg ggcccgccag
      181 gacccgatcg cgctggccct ggcggccaag tttcccggcg tgcgcatgcc ctacctgcag
      241 atccatgcca gcgacgctgc cgatctcatg tcctatatcg acgccaagtc caagcagcag
      301 cagccaacga ttgcgcttga gagcctttac gccttgacca cccaggacgg tggccacctc
      361 cgggcggccg acctcaaggg ccagcccttt gccgtggcct tcggctacac gcactgcccc
      421 gacgtctgcc cgacgacgct gctcgaatgg tcaaacctga tgcgagcggc aggcgcggac
      481 ctcggcgcct tcaagctgct gttcgtgtcg gtcgacgagg agcgcgatac gccaagcgcg
      541 ctcaaggcga tgctggcgtc cttcgatccc cgcatcaccg cgctgaccgg cagtgccgcg
      601 gaaattgccg cggtggcgcg tcagttcggt gccttctacg aaaaagtaaa gggtgcggac
      661 ggggagtaca gcatcgacca taccgtcaag agctaccttg tcgatcagga ggcgcacgtg
      721 gcgggcagca tcgatcccca aacgcccgag cccgatcagc agcgcgtctt gacgcaactg
      781 ctcgtgcgtt aggcttggcc ggcgccgcct gccggccaag acggcttccc agatcgcacc
      841 gcctcctata ctgacaaagc atgtcttggc cattcagtga cgcctttcga cggcaggtcg
      901 cgggccgttg tgcggcgttc agccgggtgg cggagcccga cggcagcgcc ggcctgaagc
      961 gggccgccgt cgccattacg ctgctgccgc tgctggaggc cggcgggccg ccgcgccagg
     1021 ccgcctttct gctgacgcgg cgggccgagg ggctgcgcgg ccacggcggc caatgggcgc
     1081 tgcccggggg gcgctgcgag gcgcacgaga gcagcgcaga ggcggcgctg cgcgagcttg
     1141 ccgaagaagt cggcgtcagc ctgcagcccg atgccgtgct cggtctactc gacgactacc
     1201 cgacgcgctc gggctacctg atcacccccg tcgtgctctg ggcgggcccg ggtcaggtgc
     1261 cgcgcgcgga tcccaacgag gttgcctcca ttcatcgcat cggcttgagc gagctcacgc
     1321 gccaggatgt cgccgatttc gtcagcattc cggaaagcga ccggcgcgtc atccgcttgc
     1381 ggatcaacgg cggttacgtg cacgccccca cggccgccgt gctctaccag ttcggcgagc
     1441 tgctgcaggg tcggaccacg cgcgtggccg acctggaaca gccggtgttt gcctggcgct
     1501 gaccgcgcgg cggctcacgg caaaattacg cccttgccgg tcgtttgcgt gtcgaacagg
     1561 cgataggcag cctcggcctg gtcgagccgc cagcggtggg tgaacagctt ctcgacggcg
     1621 atgttgtgat cggcaacgaa ttcggcgcag tccgcttgtc cttgcgtgga gaaggtccac
     1681 gatccgacca gcgtgatctg gcgacgcaac agatctgggc tgacatcgag cgtcacctgc
     1741 ccgcgctcgc cgacgtagca tgccgtgccc catgcgcgtg tggcccgcac cgcggcggcg
     1801 cgggcttcgg ccgcccctga ggtgtcgagc gtcttgtgtg cgccctcgcc gtgcgtgaga
     1861 tcgcgaatgg cggcaaccgg atcgttggag cgcgcatcga tcacctcgtg ggcgccgaac
     1921 tcgcgcgcca gctgccggcg ttcggcggag atatcaaggg cgatgacgcg ggcacccatt
     1981 gccaccgcca gctgcgttgc cgacaagcca accggtcctt gcccgaagat ggcgatggtc
     2041 tccccaccct gcagattgct gcgcttgagc gcgccgtagg ccgtgcccgt gccacaggag
     2101 atggcggccc cggtctcaaa ggaaagcgca tcgggcagcg gcaccaaggt tgtcaccggt
     2161 gccagcatgt acttggcatg cccgccgttg ccgctggcgc cgaacaccac ggccccatcc
     2221 aggcacattt gcgtccagcc ggtgcggcag tgtttgcagc tgccgcagcc cttgtagtgg
     2281 tgatccatta cgcgttggcc gatcttggcc tcgtgcggcg ccacgccggt gcccaacgcc
     2341 accaccaccc cacacggctc gtggccggcg atcaccggct cgctgccgcg cttgaacccc
     2401 gattgcgtat cgcccggctt gaaggcggcg cgatagacgt gcaggtcgct gccacacatg
     2461 cccgaggctt tgatctcgat gacgacgtcg cgcgggccgg gtgtgggatc gggaaagtca
     2521 cgcaattcaa gcttccgatc gccgagaaag acgacaccct tcataccaac ctc
//
LOCUS       DASTMT010000554         6382 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_651432, whole genome shotgun sequence.
ACCESSION   DASTMT010000554 DASTMT010000000
VERSION     DASTMT010000554.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 6382)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6382)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6382
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_651432"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..742)
                     /locus_tag="VFR00_12650"
     CDS             complement(<1..742)
                     /locus_tag="VFR00_12650"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HEU0160164.1"
                     /translation="MQVAVQPGGRSILQDLVSAPRAWHGPDLSEDDFKIELGAAALGE
                     LDAVVAEQRKAPVPTLLLEPKHFTMEACRALMAEARQRLDAGLGFVIFDRLPVARWSP
                     QEVIDVYWLLASLLEVPVAQEWKGTMIYDVRWDGQTYGADTRTALTPEGLDMHNDSSM
                     GEAPANYVCLLCLKTAKSGGMSSLASAYAAHNHFVRNHPQLLPRLYAPFYRDKQEYQA
                     PDANNWYPIFAAEEAGLRIRFNARVIRRG"
     gene            complement(807..1670)
                     /locus_tag="VFR00_12655"
     CDS             complement(807..1670)
                     /locus_tag="VFR00_12655"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEU0160165.1"
                     /translation="MDDRKAAIRAAIEQRFGATPTTPSASEAPTTWPQVWADLASRGS
                     CRAFTAQTVAPELIELLCALALSSPSKSDLQQRDIVIIEDAHIRGTIDRLLATGPLAQ
                     AWIPGAPALLIFCGNNRRQRQLHAWRGKPFANDHLDPFFNAAVDAGIALSAFVIAAEA
                     AGLGCAPISAIRNHAREISELIGLPDHVFPVAGLEFGWPSRTGRISMRLPLQATVHHN
                     RFDETRIQDDVEAYDRRRHAAQPIQRQRDVESFGQAEFYGWSEDKVRQYASPERADWG
                     AFVRAKGFRLD"
     gene            complement(1707..2783)
                     /locus_tag="VFR00_12660"
     CDS             complement(1707..2783)
                     /locus_tag="VFR00_12660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013165026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU0160166.1"
                     /translation="MRQIEVAVIGTGWCGGIRAETLARSALVDKLHVCEIRPDRLAEI
                     ARLTQAASATLDYRDIIANPRIEAVYISTTPEGTHYPIARDCLSAGKHVLLEKPIALS
                     LYEADELIQLARRGNLKFTIGYSQRFNSKIAYAKKKLGDATLGRPVSVMVSRHLSRSL
                     GKKIASRVRLSPAAMESTHDLDFVFWLLEPAKPIRVYSQGAYGYMQPINGSYDIMWTT
                     VTMDNGVLVAIGGGWNLPPGYPNYCATWIEITGTEGSLFLDDTQRDNWLATVAHGTQF
                     PMSTMPGEQVDHVFAGQMGPETLHFLESILLDRPVMVTPESARTVMEVYCAADLSAER
                     NEPIDLPLSNAALAGIADLAEARR"
     gene            3000..3974
                     /locus_tag="VFR00_12665"
     CDS             3000..3974
                     /locus_tag="VFR00_12665"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEU0160167.1"
                     /translation="MDRRRFMAGGAASAAALSTLGAVAQEPYPTRPITIINPFPPGGA
                     ADVVARPFAAVLEPILKQPVVIETKAGAAGQVGAQFAASAAPDGYTLLVHIVSISGFA
                     EVDRVFGRTPKFTRADFVPIARFTEGPMVLLVNEHTPYKTLQELVDDAKKRPNQIIFS
                     SSGLYGALHLPTALLAKAAGIEMRHLPTNGGGPALTALLGNNAQVLVSSVAAANAHIK
                     AGKARALACFSAKRAQALPQVPTLKELGLDVEFSLWVGLFAPKGTPDAIIAKLREEAR
                     KAAASEQFAKAIDNIGDVLAYQDQPEFAKFWDEDAKRVEDAVRSIGKV"
     gene            4015..4458
                     /locus_tag="VFR00_12670"
     CDS             4015..4458
                     /locus_tag="VFR00_12670"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HEU0160168.1"
                     /translation="MQLRRDHVAGGAMLIAGALVLAASTDLPFGTLASPGAGMLPTVV
                     VVFLMAFAGVLLLGAGTSPPIGAIAWTDGAHALRVMCATAAAVALYVPLGFIVSIALL
                     LFGLTFLVERKSLLSSLAFSAGATLLAYLVFASALNAPLPRGLWH"
     gene            4462..6000
                     /locus_tag="VFR00_12675"
     CDS             4462..6000
                     /locus_tag="VFR00_12675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018386975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HEU0160169.1"
                     /translation="MEATFDSLLLGFSVALRPDVLFYAFLGCLVGTLVGVLPGIGPLA
                     GISILLPVTFGLDATKAIVMLAGIYYGSQYGGSTTSILMRIPGEASSVMTCIDGYAMA
                     QRGRAGAALTIAAVGSFVAGTMGVVLLTAVAPPIAKFALGFGPPEYTALLVLGLVFLA
                     YMSSTGLPRTLLMAAVGLLLGCIGIDVMTGHFRYSFDIKELGDGIGIVPVAVGLFGLG
                     EILATPSKTVTGKVAPPRLAELLPNREEWRQSALPIARGSVLGFLVGIVPGSAHIIAS
                     FLSYAVEKRLARRPEEFGKGAVAGVAGPESANNSASTGAFVPMLALGLPTGPVTAVLM
                     AALLIHGVPPGPMLVNEHPQVFWGFVASMYVGNLMLLALNLPLVGVFVTVLRIPYSYL
                     YPLIIMFSIIGVYEVNHSIVDVWIMLIMGVVGYLLRKFDFDPAPLVLGLVIAPIFEMS
                     LRQSLIMSNGNWLIFLERPIAQALLAVSVVLLGLSALSLIMKRRDWRAKLAQAEAGEG
                     ADGG"
     gene            complement(6029..6340)
                     /locus_tag="VFR00_12680"
                     /pseudo
     CDS             complement(6029..6340)
                     /locus_tag="VFR00_12680"
                     /inference="COORDINATES: protein motif:HMM:NF039588.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
BASE COUNT          974 a         2058 c         2139 g         1211 t
ORIGIN      
        1 agccccgccg gatgacgcgc gcattgaagc ggatcctgag cccagcctcc tccgctgcga
       61 agatcgggta ccagttgttg gcgtcagggg cctgatactc ctgcttgtca cggtagaacg
      121 gcgcatagag gcgcggcagc agctgcgggt ggttgcgcac gaaatggttg tgcgccgcgt
      181 aggcactcgc gagcgagctc atgcccccgg atttcgccgt cttgagacaa agcaaacaga
      241 catagttcgc gggcgcctcg cccatcgagc tgtcattgtg catgtcgagg ccttccggcg
      301 tcagcgccgt gcgcgtgtcg gcaccgtagg tctgcccgtc ccagcgcacg tcgtagatca
      361 tcgtgccctt ccattcctgg gcgacgggca cctcgagcag gctggcaagc aaccaataga
      421 cgtcgatcac ctcttgtggg ctccagcgcg ccaccggcag acggtcgaaa atcacaaagc
      481 cgaggccagc atcgaggcgc tggcgggcct cggccatcag ggcgcgacag gcctccattg
      541 tgaagtgctt gggttccagc agcagggtcg gcaccggcgc tttgcgctgc tcggcaacga
      601 ccgcgtcgag ttcgccaagc gcagcagcgc cgagctcgat cttgaagtca tcctcgctga
      661 ggtcgggccc gtgccaggcg cgtggcgcgg acaccagatc ttgcaaaatg ctcctgccgc
      721 caggctggac cgcgacctgc atgcctcgtc tccttcccag gcatcggcgc gcctgcgcca
      781 gagcgcccgc ggcgacgcgg ctcgcattag tcgagccgga agcctttggc gcgcacgaac
      841 gcgccccagt cggcccgctc ggggctagca tactggcgca ccttgtcctc cgaccagcca
      901 tagaattccg cctggccgaa gctttcgacg tcgcgttgtc gctggatcgg ttgcgccgca
      961 tgccggcggc ggtcgtaagc ctcaacgtcg tcttggatgc gggtctcgtc gaagcggttg
     1021 tgatgcacgg tggcctgcag cggcaagcgc atgctgatgc ggcccgtgcg gctcggccag
     1081 ccgaactcga gacccgcgac cgggaagacg tggtcgggca agccgatcag ctcggagatc
     1141 tcgcgcgcat gattgcggat ggcgctgatg ggagcgcagc caaggcccgc ggcttcggcg
     1201 gcgatcacga acgccgagag cgcaatgcct gcgtcgacgg ccgcattgaa aaagggatcc
     1261 aggtgatcgt tggcgaaggg tttgccgcgc caggcgtgca gctggcgctg ccggcggttg
     1321 ttgccgcaga aaatgaggag cgccggggcg ccgggtatcc aggcctgggc gagggggccg
     1381 gtggcgagca ggcggtcgat cgtgccgcgg atgtgggcgt cctcgataat gacgatgtcg
     1441 cgttgctgca ggtcgctttt gctgggcgac gagagcgcca gggcgcacaa cagttcgatg
     1501 agctcgggcg ccaccgtttg tgccgtgaag gcgcggcaag agccgcggct cgccaggtcc
     1561 gcccagacct gcggccaggt ggtgggggct tcgctggcac tgggcgtcgt gggggtcgcg
     1621 ccgaagcgct gctcgatggc ggcgcggatc gcggctttgc ggtcatccat gctgggtcat
     1681 ccgcttcaag tgttatccca ccgggcctaa cgccttgcct cggccaagtc ggcaatgccg
     1741 gccagcgccg cattggaaag cggcagatcg atcggttcgt tgcgctcggc cgagagatcg
     1801 gcggcgcagt agacctccat caccgtgcgc gcgctttcgg gcgtcaccat taccggccga
     1861 tccaacagaa tggattccag gaaatgcagc gtctccggtc ccatctgtcc ggcaaagacg
     1921 tgatccacct gctcgccggg catggtcgac atgggaaact gcgtgccgtg cgccacggtg
     1981 gcgagccaat tgtcgcgctg cgtgtcgtcg agaaacagcg aaccttccgt cccggtgatc
     2041 tcgatccagg tggcgcagta gttgggatag cccggcggta ggttccagcc gccgccgata
     2101 gcgaccaaga cgccgttgtc catggtcacc gtcgtccaca tgatgtcgta ggaaccgttg
     2161 atcggctgca tgtagccgta cgcgccctgc gaatagacgc ggatcggctt ggccggctcg
     2221 agcagccaga acacgaagtc gaggtcgtgc gtggattcca tggccgccgg tgacaagcgc
     2281 acgcgcgagg cgattttctt gccgaggctg cgcgaaagat gccggctcac catcacgctc
     2341 accggtcggc ccagcgtggc atcgccgagc tttttcttgg cgtaggcgat cttgctgttg
     2401 aagcgttggg agtagccgat tgtgaatttg aggttgccgc gccgggccag ttggatcagc
     2461 tcgtccgcct cgtagaggct cagcgcgatg ggcttttcca acaggacgtg cttgccggcg
     2521 ctgaggcagt cgcgggcgat cggatagtgc gtgccttccg gcgtggtgga gatgtagacc
     2581 gcctcgatgc gcggattggc gatgatgtcg cgatagtcga gggtagcgct ggccgcctgc
     2641 gtcaggcgcg cgatctccgc caggcggtcg ggacggattt cacagacgtg cagcttgtcg
     2701 accagcgccg agcgcgccaa agtctccgct cgaatgcccc cgcaccaacc ggtgccgatc
     2761 acggccactt cgatttgcct catcgcctcc tccttgagcc gaggcgagtt agccgttggc
     2821 cgtggcagtt gtccagcgcg cagcccggcg ccgcccgctg gccgccccag cgctctatcc
     2881 ttgaccagcc agcatcggcc tcagtagcat cccccgcggt cccggcgcgg cagaagaccg
     2941 tcgcggggcc gaggccatgc ctaagacaag gcgcggcgca aaagcccaag ggaggacgca
     3001 tggataggcg caggtttatg gcgggtgggg cggcctcggc ggcggccctg tcgacgctgg
     3061 gcgccgtcgc gcaagagcct tacccgaccc ggcccattac gatcatcaat ccattcccgc
     3121 cagggggcgc cgccgacgtg gtggcgcggc catttgcggc ggtgctcgag cccatcctga
     3181 agcagccggt tgtgatcgag accaaggcgg gtgcagccgg gcaagtcggg gcccagtttg
     3241 ccgccagcgc ggcacccgat ggctacacgc tgctcgtgca catcgtctcg atttcgggtt
     3301 ttgccgaggt ggaccgggtg ttcgggcgaa ccccgaagtt cacgcgcgcc gatttcgtgc
     3361 cgatcgcgcg ctttaccgag gggccaatgg tgctgctggt caacgagcac acgccctata
     3421 agaccttgca ggagctggtt gatgacgcca agaagcgacc caaccagatc atcttcagct
     3481 cgtctgggct ctacggggcc ctgcatctgc cgaccgcgct gctcgccaag gccgccggta
     3541 tcgaaatgcg gcacctgccg accaacggcg gcggcccggc gctgacggcc ttgcttggca
     3601 acaacgcgca agtgctcgtg tcctcggtgg cggccgccaa cgcccacatc aaggccggca
     3661 aggcgcgcgc gctcgcctgt ttctccgcca aacgcgcgca agcgctgccg caggtgccaa
     3721 ccctgaaaga gctcgggttg gacgtcgagt tctccttgtg ggtcggcctg tttgccccca
     3781 agggtacgcc cgatgcgatc atcgccaagt tgcgcgagga ggcgaggaaa gccgcggcga
     3841 gcgagcagtt cgccaaggcg atcgacaaca tcggcgacgt tctcgcctat caggatcagc
     3901 cggagttcgc gaaattctgg gacgaggatg ccaagcgcgt ggaagacgcg gtgcgatcga
     3961 tcggcaaggt ttgacgaagc gggcagcggg caggctcgag cgacgaggac ggcggtgcag
     4021 ctgcgcaggg atcatgtcgc cggcggtgcg atgctcatcg ccggcgcgct cgtattggcc
     4081 gcaagcacgg acttgccgtt cggcacgttg gcctcgcccg gcgcgggcat gttgccgacc
     4141 gtggtggtgg tctttctcat ggccttcgcc ggcgtcctgt tgctcggcgc cggcaccagt
     4201 ccgccgatcg gcgccattgc ttggacggat ggggcccatg cgctgcgcgt gatgtgcgcg
     4261 acggcggccg ccgtcgccct ttacgttccc cttggcttta tcgtcagcat tgccctgttg
     4321 ctgttcgggc tcaccttttt ggtggagcgc aaatccctcc ttagctcgct tgcctttagt
     4381 gctggcgcca cgctgctggc gtatctggtg tttgccagcg cgctcaatgc gccgctgccg
     4441 cgcggtttat ggcactaacc gatggaagcc accttcgaca gcttgctcct cggcttctcc
     4501 gtggccttgc gtccggacgt gctgttttat gcctttctcg gctgcctggt cggcacgctg
     4561 gtcggcgtcc tgccgggcat cggcccgctc gccgggatct ccatcctctt gccggtgacc
     4621 tttggtctcg acgcgaccaa ggcgatcgtc atgctggcgg gcatttacta cggctcccaa
     4681 tatggcggct cgaccacctc gatcctgatg cgtatccccg gcgaggcctc atccgtcatg
     4741 acctgcatcg acggctacgc catggcgcaa cgcgggcggg ccggggctgc cctcaccatc
     4801 gccgcggtcg gctcgttcgt tgccggcacc atgggcgtcg ttctgctgac cgccgtggcg
     4861 ccgccgatcg ccaaatttgc gcttggcttc gggccaccgg agtatacggc gctcctggtg
     4921 ctgggcctcg tcttcctcgc ctatatgtcg tcgacgggcc taccgcgcac gctgctgatg
     4981 gcagccgtcg gtctcctgct cggctgcatc ggcatcgatg tgatgaccgg gcattttcgc
     5041 tacagcttcg atatcaagga gttgggggat ggcatcggca tcgtgccggt cgccgtcggt
     5101 ctcttcgggc tgggcgaaat cctggccacc cccagcaaaa cggtgacggg aaaggtggcg
     5161 ccgccgcggc ttgccgagct gttgcccaac cgtgaggaat ggcgccagtc ggccctgccg
     5221 atcgcgcgcg ggtcggtgct cggttttctg gtcggcatcg tgcccggctc cgcccacatt
     5281 attgcgagct ttctgtccta tgccgtcgaa aagcgcctcg ccaggcggcc cgaggagttc
     5341 ggcaagggtg ccgtggcggg cgtcgcgggg ccggaatcgg caaacaactc ggcttcgacc
     5401 ggcgccttcg tacccatgct ggcgctcggg ctgccgaccg gccctgtcac ggccgtgctc
     5461 atggccgcct tgcttatcca tggcgtgccg ccgggtccca tgctggtcaa cgagcacccg
     5521 caggtgtttt gggggtttgt cgcctccatg tatgtcggca acctgatgtt gctcgccctc
     5581 aatctgcccc tggtcggcgt gttcgtgacg gtgctgcgca ttccctacag ctatctttat
     5641 ccgctgatca tcatgttctc gatcatcggt gtttacgagg tgaaccactc gatcgtcgac
     5701 gtctggatca tgctgatcat gggcgtggtc ggctacctgt tgcgcaagtt tgatttcgat
     5761 cccgcgccgt tggtgctggg cttggtgatc gccccgatct tcgaaatgag cctgcgtcag
     5821 tccctgatca tgtcgaatgg caactggctc attttcctgg agcggccgat cgcgcaagcg
     5881 ttactcgcgg tcagcgtcgt gctgctcggc ctctccgccc tctcgctcat catgaagcgt
     5941 cgcgactggc gcgccaagct ggcgcaggcg gaggccggcg aaggcgccga cgggggctga
     6001 gcgcgcatga ccgccgcggc ggcgcagctc agtcgtcgct ggcgtgcccg cgtttgggga
     6061 ccagcaccgt gcgcgtgaag ctcatgaaca cggtgccgtc ggctttctta ccggtcgtgc
     6121 gcacctccac gatgccttgc gtcggccggc tcgcggattg gcgcttggaa agcacttcgg
     6181 tttccgcata gatcgtgtcg ccgatgaaga cgggcgcggt gagacgaata tcgctccagc
     6241 ccaaattggc aattgccttt tgactaacgt cgctcacgct catgccgacc acgatcgaca
     6301 gcgtcaggca gctgttgacc aggcattggc cgaattccga cttcgccgcg aagccggtag
     6361 gccctcagcg ctggcggcag ag
//
LOCUS       DASTMT010000555         7364 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_3272457, whole genome shotgun sequence.
ACCESSION   DASTMT010000555 DASTMT010000000
VERSION     DASTMT010000555.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 7364)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7364)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7364
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3272457"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..709)
                     /locus_tag="VFR00_12685"
     CDS             complement(<1..709)
                     /locus_tag="VFR00_12685"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HEU0160170.1"
                     /translation="MADTTPYHDRDGSIWYDGKLVPWREAGLHVLSHGLHYASAVFEG
                     ERAYHGVIFELTQHSQRLVEGAKTLDFEIPYSVAEIDQACRDVVKANKLSDCYVRPIA
                     WRGSEMMGVSAQATKIHLAIAAWDWGSYFPIEQRLKGIRLTLANYRRPDPATAPAKTK
                     AAGLYMICTIEKHRAEKMGYADALMYDWRGHVAECTGANIFLIKEGKLHTPTPDCFLD
                     GITRRTVIALAKKRGYEV"
     gene            922..1497
                     /locus_tag="VFR00_12690"
     CDS             922..1497
                     /locus_tag="VFR00_12690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018387129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEU0160171.1"
                     /translation="MTDIISVPARRLQAPAQVRAEAGTGTRAAAPAEAELITCVELLF
                     FAYRDFTGEADAVLAEFGLGRAHHRVLHFVNRNPGLKVQDLLAILKITKQSLARVLKL
                     LLDQGWIQQVAGDADRRQRLLHTTPKGAALADRLDALQAKRIGKALAAAGAGSETAML
                     RFLFAMIVPEERAFVEALLPRPIEAAQPPAG"
     gene            1527..2216
                     /locus_tag="VFR00_12695"
     CDS             1527..2216
                     /locus_tag="VFR00_12695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU0160172.1"
                     /translation="MEPLADNAAHILIVDDDQRIRDLLARYLFEHGFRVSTATDATSA
                     RAAMRGLAFDVVILDVMMPGESGLELARGLKAISNIPICMLTARAEPEQRIEGLEIGV
                     DDYVAKPFEPRELLLRLQNILKRGRGPSGPRDEIRMGDFIFHVGRGELRRNDETIKLT
                     ERERDLLRLFAQRPGSPIARHELASDDSTGSERAIDVQINRLRRQIETDPANPVFLQT
                     VRGKGYILHTD"
     gene            2232..3641
                     /locus_tag="VFR00_12700"
     CDS             2232..3641
                     /locus_tag="VFR00_12700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU0160173.1"
                     /translation="MAIAHKLVRTTETWRNRLAALSWPKGALASVSGLLAELAPKGLY
                     ARALIIIIAPIVLLESVVAFAFMERHWNQVTRRLSEATARDMAAIVSIYENSEDGTRL
                     AELAQSKFGFTLEILPPGNLPTAQPKPFFDLLDRALSDELRTFVNRPFWIDTVGQSRL
                     VEVRVKLDRAILRFTAQRGQTYASNSHIFLIWMVGTSVVLLTVAIAFLRNQIRPILRL
                     AEAADAFGKGRPVPQDFRPRGAREVRQAASAFLEMRDRIRHHVEQRTSMLAGVSHDLR
                     TVLTRFKLELALLGDGPEIAALRNDVNEMQHMLEDYLAFAKGDGGEAAAPTDVGELLG
                     EVQEETEHIGAPIEVRLRHPRRPLVLPLKRQAFKRAVTNLVTNAARFGDNVVIRAAAE
                     ECWLRIEVDDDGPGIPPAERDNVFRPFYRLDHARNQDAGNSGLGLAIARDIARSHGGD
                     IALGVSSMGGLRATMRVPL"
     gene            complement(3648..4304)
                     /locus_tag="VFR00_12705"
     CDS             complement(3648..4304)
                     /locus_tag="VFR00_12705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015930206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEU0160174.1"
                     /translation="MLTIYGVYRSRASRTFWLAGELGLAFRQVPVLQAYRLPDAAARH
                     APLNTRSPEFLRINPNGHIPSIDDDGVVLHESLAINLYLAKKHGGPLGPANPAEDGAM
                     AMWALWAATEVEPHALNILYHRASYPEGQRDAAIAAAAVKTLRAPFAVLDQALAATGF
                     AVGDRFTVGDLNLAEVLRYAMPATELFQAAPRVAAWLTACQARPAFKAMMARREAEPA
                     "
     gene            complement(4368..4727)
                     /locus_tag="VFR00_12710"
     CDS             complement(4368..4727)
                     /locus_tag="VFR00_12710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018183706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HEU0160175.1"
                     /translation="MRQLGPPPSPIAFADFLKVDIRVGTIIAAEPLAGARKPALRLDI
                     DFGPELGVKRSSAQITAHYLPAALIGRQVAAVVNFPPRQIAKTLSQVLTLGFPDAAGE
                     VVLITPERSVPNGGRLF"
     gene            complement(4748..5428)
                     /locus_tag="VFR00_12715"
     CDS             complement(4748..5428)
                     /locus_tag="VFR00_12715"
                     /inference="COORDINATES: protein motif:HMM:NF020100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEU0160176.1"
                     /translation="MLDQSTPKGRILAAALALAAAKDWAQVSLLDIAQEAKVPLGEMR
                     DLLASKAAIIAELLRAVDREVLSRTERVAGQEKRDLLFDVLMTRFDVLSPHKAAIKSM
                     AKSAGADLALAGPLLASQHWMLEAAGIDTSGPGGGLRVAGLAGVYASVFRTWLEDEDP
                     GHARTMAALDRRLRRGERTLNQLDAVAAGVERLATAVPQFLRALGEAGRKARAPDDAT
                     AATEEPKS"
     gene            5583..6728
                     /locus_tag="VFR00_12720"
     CDS             5583..6728
                     /locus_tag="VFR00_12720"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020083967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HEU0160177.1"
                     /translation="MLEVMSPSSDYIDIPDSDPLRLDCDKLLAPVRIAYKTYGTLNPD
                     KSNAILICHALTLDQYVASEQPVTRKPGWWEAMVGPGKPIDTDRFFIICPNILGGCMG
                     STGPASLNPNTGRPWGLEFPVITIGDMVDAQVRLIDRLGIDQLFSVVGGSMGGMQVLE
                     WVARHPERVFTAIPIATAAWHSSQNIAFHEVGRQAVMADPDWCQGRYLEFGKVPRNGL
                     AVARMAAHITYLSEEALHRKFGRSLQDLAAKTFSFNADFQVESYLRHQGSTFVDRFDA
                     NSYLYITRAMDYFDLAAEHGDVLANASRGCKTRFCVVSFTSDWLYPTRESREIVQALN
                     AVAANVSFVEIESDKGHDAFLLEEPELFATIGGFLNAAAAKRGSRRA"
     gene            6728..>7364
                     /gene="metW"
                     /locus_tag="VFR00_12725"
     CDS             6728..>7364
                     /gene="metW"
                     /locus_tag="VFR00_12725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018268941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HEU0160178.1"
                     /translation="MLAPSRPALHRVDHLLIAEMVRAGSRVLDVGCGDGALLQLLADT
                     KNVDGRGIEVARERVNACVARGLSVIQGDADRDLADYPDAAFDYAILSLTIQATRYPK
                     SVLENLLRIGHRAIVSFPNFGHWQVRGQLLFTGRMPVTENLPEAWYASPNAHLCTIKD
                     FADLCRVTGAGVERAVAFNLKGQKLGTWLPLTVHNFLGEKAVFMLTRHVPPQ"
BASE COUNT         1271 a         2440 c         2374 g         1279 t
ORIGIN      
        1 ccacctcata gccccgcttc ttggcgagcg cgatcaccgt gcgccgcgtg atgccatcca
       61 aaaagcagtc gggcgtgggg gtgtggagct tgccctcctt gatcaagaaa atgttggccc
      121 ccgtgcactc ggcgacgtga ccgcgccagt cgtacatcag ggcgtccgca taacccatct
      181 tctcggcccg gtgtttttcg atggtacaga tcatgtagag gccggcagcc ttggttttgg
      241 ccggggccgt agccggatcg ggtcgccggt aattggcgag cgtcagccgg atgcccttca
      301 agcgctgctc gatcgggaaa taggaacccc agtcccaggc ggcgatggcg aggtgaatct
      361 tggtcgcttg cgccgatacg cccatcattt cgctgccgcg ccaggcgatt ggccgcacgt
      421 aacaatcgct cagcttattg gccttcacca cgtcgcggca ggcctgatcg atctcggcca
      481 cgctgtaggg gatttcgaaa tcgagggtct tggccccctc caccaggcgc tggctgtgct
      541 gggtcagctc gaagatcacc ccatggtagg cgcgctcgcc ctcgaacacc gcgctggcgt
      601 agtgcaggcc gtggctcagc acgtgcaggc cggcctcacg ccatggcacc agcttgccgt
      661 cgtaccaaat ggagccgtcg cgatcgtgat aaggggttgt atcggccatg cctcggggtc
      721 tccgcggacg ctgtgcccca ccccccggag cctcgccacc aggccttggc tctcgccacc
      781 atgccttggc taaggacccc tggcggggca ggggattgcc caatttgcaa gccttttcgc
      841 cgcccaaact ggcgcacaaa gaggcatgca ggcctgtaaa atgtcttgcc agcactaaca
      901 atcggggtca aataagtcaa catgactgac ataatttccg tgccggcacg gcgcttacag
      961 gcaccggcgc aagtccgcgc cgaggcgggc accgggacgc gcgctgcggc gccggccgag
     1021 gccgagctca ttacctgcgt ggagctactg ttctttgcct atcgcgactt taccggtgag
     1081 gcggacgcgg tgctggcgga gttcgggctg gggcgggcgc accaccgcgt gctgcacttt
     1141 gtcaaccgca accccggcct gaaggtgcag gatttgttgg cgatcctgaa gatcaccaag
     1201 caaagcctgg cgcgcgttct caagctgctg ctggatcagg gctggatcca gcaggtcgcc
     1261 ggcgacgccg atcgccgcca acgcctgctg catacgacgc ccaagggcgc ggcgttggcc
     1321 gaccggctcg atgccttgca ggcaaagcgc attggcaagg cgctcgccgc ggcgggggcg
     1381 ggcagcgaga cggcgatgct gcgcttcctt tttgccatga ttgttccgga agaacgcgca
     1441 ttcgttgagg cacttttgcc gcgaccgatc gaggccgcgc agccgccggc gggttagccg
     1501 cggcacggca acgggggact ggggccatgg aaccgttggc cgacaatgcg gcgcacatcc
     1561 tgatcgtcga cgacgatcag cgcatccggg acctgctggc ccgctatttg ttcgagcacg
     1621 gtttccgcgt cagcacggcc accgatgcca ccagcgcccg cgccgccatg cgcgggctcg
     1681 ccttcgatgt cgttattctc gacgtcatga tgcccggcga gagcgggctt gagctcgcgc
     1741 gcggcctgaa agcgatttcc aacattccga tctgcatgct gacggcacgt gccgagccgg
     1801 agcagcgcat cgagggcctc gagatcgggg tcgacgacta tgtggccaag cctttcgagc
     1861 cgcgcgagct gttgctgcgg ctgcagaaca tcctcaagcg gggccgcggg ccgtccggac
     1921 cgcgcgacga aatcaggatg ggcgatttca tctttcatgt ggggcgcggg gagctcaggc
     1981 gcaacgacga gaccattaag ctcaccgagc gcgagcgcga cctgctgcgg ctgttcgccc
     2041 agcgcccggg ctcgccgatc gcgcgccacg agttggcgag cgacgactcc accggcagcg
     2101 aacgcgccat cgacgtgcag atcaatcgct tgcggcgaca aatcgagacc gatccggcaa
     2161 acccggtctt tctccagacg gtccgcggca aaggctatat actgcatacg gattgagagg
     2221 taggcgcttc gatggcgata gcccacaagc tcgtccgcac gaccgaaact tggcgcaacc
     2281 ggcttgccgc gctgagctgg cccaaaggcg ccttggcgag cgttagcggg ctgctcgccg
     2341 agttggcccc gaaggggctt tacgcgcgcg ccctcatcat catcattgcc cctatcgtgc
     2401 tactggaaag cgtggttgcc ttcgccttca tggagcggca ttggaaccag gtgacgcgcc
     2461 gcctgtcgga ggccacggcg cgcgatatgg ccgcaatcgt ttccatctac gaaaattccg
     2521 aggatggaac gcgccttgcc gagctggccc agagcaagtt cggcttcacc ctggaaattc
     2581 tgcctcccgg caatctgccg acggctcagc ccaaaccgtt tttcgatctc ctcgaccggg
     2641 cgctttccga tgagctgcgc acctttgtca atcggccgtt ctggatcgac acggttggcc
     2701 agtcccggct ggtggaggtg cgcgtcaagc tcgatcgggc catcctgcgc ttcacggcgc
     2761 agcgcggtca gacctacgct tccaattcgc acatcttcct gatctggatg gtgggcacct
     2821 ccgtcgtgct cttgaccgtg gcgatcgcct tcctgcgcaa ccagatccgc ccgatcctgc
     2881 gcctggccga agccgccgac gccttcggca agggccgccc ggtgccgcag gacttccgac
     2941 cgcgcggcgc gcgcgaggta cgccaagcgg cttccgcctt tttggagatg cgcgatcgca
     3001 tccgccacca cgtcgaacaa cgtacctcca tgctggccgg ggtgagccat gatctgcgca
     3061 ccgtcctgac gcgcttcaag ctcgagttgg cgctgctcgg cgacggcccg gaaatcgccg
     3121 ccttgcgcaa cgacgtcaac gagatgcagc acatgctgga agactatctg gccttcgcca
     3181 agggcgatgg cggcgaggcg gcggccccga ccgacgttgg cgagctgctc ggcgaggtgc
     3241 aggaagagac cgaacacatc ggagccccga ttgaggtcag gctgcgccac ccgcgccgcc
     3301 cgctggtgct gccgctgaaa cgccaggctt tcaagcgggc ggtgaccaat ctggtcacca
     3361 atgccgcccg tttcggcgac aacgtcgtca ttcgtgccgc ggcggaggag tgctggctgc
     3421 gcatcgaggt cgacgacgac ggtccgggca ttccgccggc cgagcgagac aacgtctttc
     3481 gtcccttcta tcggctcgat cacgcgcgca accaggacgc cggcaattcg ggtttggggc
     3541 tggccattgc ccgcgacatc gcgcgcagcc atggcggcga tatcgcgctt ggcgtcagct
     3601 ccatgggtgg cctcagagcc accatgcgcg tgccgctgtg aggcggctca ggccggttcg
     3661 gcctcacgcc gcgccatcat ggctttaaag gccggccgtg cctggcaggc cgtgagccag
     3721 gctgcaacgc gcggcgccgc ttggaacagc tcggtagcgg gcatcgcgta gcgcaagacc
     3781 tccgccagat tgaggtcgcc aacggtgaac cgatcgccga cggcaaaccc ggttgccgcc
     3841 agcgcctggt ccaacaccgc aaagggcgcg cgcaaggttt taaccgccgc cgcggcgatg
     3901 gcggcgtcgc gctgcccctc ggggtagctc gcccggtgat agagaatgtt gagggcgtgc
     3961 ggctcgacct cggtcgccgc ccaaagcgcc cacatggcca tggcaccgtc ttcggcgggg
     4021 ttcgccggcc ccagcggacc gccgtgcttc ttggccaagt agaggttgat cgccagggat
     4081 tcgtgcagca cgacaccgtc atcgtcgatg ctggggatat gcccgttggg attgatgcgc
     4141 aggaactcgg gcgagcgcgt attcagcggg gcgtgccgcg ccgcggcatc gggcaaccgg
     4201 taggcctgca gcaccggcac ctggcggaag gcgagcccga gctcacccgc caaccagaag
     4261 gtgcgcgagg cgcgcgagcg ataaacgcca taaatcgtca gcatgggctt cctctctttt
     4321 tgctagcacg ccgcccggcg aggtcaccgg cgcgcaaacg cccagggcta gaacagccga
     4381 cctccattgg gcacgcttcg ctccggtgta attagcacca cctcgccggc cgcgtcgggg
     4441 aagccgagcg tcaggacctg cgagagcgtc ttggcgatct ggcgcggggg aaagttgacg
     4501 acggcggcaa cctgccggcc gatcagcgcg gcgggaagat agtgcgcggt aatctgcgcg
     4561 gagctccgct tgacgccgag ctcaggtcca aaatcaatgt cgagccgcag cgcgggcttg
     4621 cgcgcgccgg caagcggttc ggcggcgatg atggtgccga cacggatatc gaccttgaga
     4681 aaatccgcaa acgcgatggg agacggcggc gggccgagct ggcgcattga tcctccaggg
     4741 acaagcctta actcttcggc tcctcggtgg cggccgtcgc gtcgtccggc gcgcgggctt
     4801 tgcggccggc ttcgccgagc gcgcgcagaa actgcggcac ggcggtggcg aggcgctcga
     4861 cgccggcagc gacggcatca agctggttga gggtgcgctc gccgcggcgc agccggcgat
     4921 cgagcgccgc catggtgcgc gcgtgcccgg gatcctcatc ctcgagccag gtgcgaaaca
     4981 ccgaagcata gacgccggcc aatccggcca cccgcagccc accgcccggt ccactggtgt
     5041 cgatgccggc cgcctcgagc atccagtgct gtgaggcaag gagcggcccg gccagcgcca
     5101 gatcggcgcc ggccgatttg gccatggatt tgatggcggc cttgtgcggt gagagcacgt
     5161 cgaaacgggt catcaggacg tcgaacaaaa ggtcgcgctt ttcttggccc gcaacgcgct
     5221 cggtccggct caggacctct cgatcaacgg cccgcaaaag ctcggcaatg atcgcggcct
     5281 tgctggcgag gagatcgcgc atttcgccca gcggcacctt ggcctcttgg gcaatgtcca
     5341 acaggctcac ctgcgcccag tccttggcgg cggctaaggc cagggcggcc gccagaatgc
     5401 ggcccttcgg ggtggattga tcgagcatgg agcacctcct cgctcccatt acatcgtgcg
     5461 cggtggcgat ttgcaaaagg cgcgtgcaaa gaattcattg acattcgcgc ccgagagcct
     5521 agctaacagc ccctatgaac agggcagatc cacgcgattt tctggcatcg accgatgcgc
     5581 gcatgctgga ggtgatgagc cccagcagcg actacatcga tattcccgac agcgatccgt
     5641 tgcggctcga ttgcgacaag ctgctggcgc ccgtgcgcat cgcctacaag acctacggca
     5701 cgctcaatcc cgacaaatcc aatgccatcc tcatctgcca cgccttaaca ctcgatcaat
     5761 atgtcgccag cgagcaaccc gtgacgcgca agcccggctg gtgggaagcc atggtcgggc
     5821 ccggcaagcc gatcgacacg gatcgcttct tcatcatctg ccccaacatt ctcggcggct
     5881 gcatgggttc gacggggccg gcgagcctca atcccaacac cggccgcccc tgggggctcg
     5941 agtttccggt catcaccatc ggcgacatgg tcgatgcgca ggtgcgcttg atcgaccggc
     6001 tcggcatcga tcagctgttc agcgtggtcg gcggatcgat gggcggcatg caggtgctcg
     6061 aatgggtcgc gcgccatccc gagcgcgtgt tcacggcaat cccgatcgca acggcagcgt
     6121 ggcactcctc gcagaacatc gcctttcacg aagtcggccg ccaggccgtg atggccgacc
     6181 cggactggtg ccaagggcgt tatttggaat tcggcaaggt gccgcgcaac ggtctggccg
     6241 tggcgcgcat ggcggcccac atcacctatc tttccgaaga ggccttgcac cgtaagtttg
     6301 ggcgcagcct gcaagatctc gcggccaaga ccttcagctt caacgccgac ttccaggtgg
     6361 agagctatct gcgccaccaa ggctccacct tcgtcgaccg cttcgacgcc aacagctatc
     6421 tctacatcac gcgcgccatg gactacttcg acctggccgc cgagcatggc gatgtcttgg
     6481 ccaatgcctc ccgcggctgc aagacccgct tttgcgtggt gtccttcacc agcgattggc
     6541 tctatcccac gcgtgaaagc cgcgagattg tgcaggccct caatgcggtg gccgccaacg
     6601 tcagctttgt cgagatcgaa tcggacaagg gccatgacgc ctttttgttg gaggagccgg
     6661 aactgtttgc caccatcggc ggcttcctca acgccgcggc ggccaaacgc ggttcgagga
     6721 gagcctgatg ctcgccccca gcaggcccgc gctgcaccgc gtcgaccatt tgctgatcgc
     6781 ggaaatggtg agggccggct cgcgcgtcct cgacgtgggc tgcggcgacg gcgcgctgct
     6841 gcagctgctg gccgatacca agaatgtcga cggccgcggc atcgaggtcg cccgtgaacg
     6901 tgtcaatgcc tgcgtggcgc gcggcctctc cgtgattcaa ggcgatgccg atcgggatct
     6961 ggccgactat cccgatgcgg ccttcgacta cgccatcctg agcctgacca tccaagctac
     7021 ccgctacccc aaaagcgtgc ttgagaacct gctgcgcatc ggtcatcgcg ccatcgtttc
     7081 gtttccgaac tttggccatt ggcaagtcag gggccagctg ctgttcaccg gccgtatgcc
     7141 cgtgacggag aatctgcccg aggcctggta cgccagtccc aacgcgcacc tatgcacgat
     7201 caaggacttc gcggatctgt gccgcgtcac cggcgccggc gtcgagcgcg cggtcgcctt
     7261 caacttgaag gggcagaagc tcggcacctg gctgccgctg acggtacaca acttcttggg
     7321 cgagaaggct gtcttcatgc tgacgcggca cgtgccaccg cagc
//
LOCUS       DASTMT010000556         9486 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_3599518, whole genome shotgun sequence.
ACCESSION   DASTMT010000556 DASTMT010000000
VERSION     DASTMT010000556.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 9486)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9486)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9486
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3599518"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            518..1171
                     /locus_tag="VFR00_12730"
     CDS             518..1171
                     /locus_tag="VFR00_12730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HEU0160179.1"
                     /translation="MYRVLGSALLSAMLAGCGMAASPLTAGFANSGQPTSSLPTRAQG
                     AGVDTAMASDPVKPALKAVDKAPKGSFEKAPQGALADRDYTKTELDLEKARQLINAYR
                     KQHGLKPLTLNAALTEAAKLHARDLAKWDRISHYGSDGSNPWDRVKRVGYRAKVAAEN
                     VGTGQVSIDEVLKGWEASPGHNKNLLLRDAEHMGIALVQDPRTEFKTFWTLLVGASL"
     gene            complement(1283..2683)
                     /locus_tag="VFR00_12735"
     CDS             complement(1283..2683)
                     /locus_tag="VFR00_12735"
                     /inference="COORDINATES: protein motif:HMM:NF014051.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HEU0160180.1"
                     /translation="MSAGAKAVAEPASFAFGAARAWPRTERRAEPRPAAVQPARCQGS
                     AARQALTVFFVRSVSAGLLYLSQIALARWMGAYDYGVYVLVWTWVLVLGNLSNLGLST
                     AVVRLVPQHRARGEDAALRGLFFAGRLLVVTVASGIALIGFGVLACLGEQVATPYLVP
                     ALLGLICLPIVTLIDIQDGMARARGLMALGLLPPYVLRPLLLLGLLAAAQAAALPVTA
                     RSGIGAAILASLGAVMVQSLLLRRQLQRDLPRGPRRYALAHWLKTALPFWLILACDLT
                     LQNADVLVVSASLGPAEAGMYFAAAKTMGLVLFVHYAVGSAMAPRFVALDARGDRAGL
                     GVAMAKATHWIFWPSLAGACAILLAGRAILALFDPQFAAAYPVMGILALGLVARAALG
                     PADVVLNMLGQGAACARIVLVAAALNVGLSVVLVPVLGLAGAAAATSLALFAAAAGNC
                     ALARRRLNVRTAIWDA"
     gene            complement(2680..2856)
                     /locus_tag="VFR00_12740"
     CDS             complement(2680..2856)
                     /locus_tag="VFR00_12740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0160181.1"
                     /translation="MVAVLEGRHGIWAADVAEFFSTLHSLKGDAGRSWAWANVADRVR
                     HRSELRQQEHAQRE"
     gene            complement(3241..4302)
                     /locus_tag="VFR00_12745"
     CDS             complement(3241..4302)
                     /locus_tag="VFR00_12745"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HEU0160182.1"
                     /translation="MLATILRGMVLATGLAALPALAGPALLLDAGTGSVLYAEDQDNQ
                     WHPASLTKIMTAYVVFEAIKAGKITPESKIGCSELANSQPPSKVGLPVGAEMTVETAL
                     QALIVKSANDVAVMLAEAVAGSQEAFVEKMNATAARLGMTRTKFVNANGLPAAEQVTT
                     ARDLAKLSVAVVKDYPEYAPLWALMDVRIGNRHLRTHNGLLANYEGADGLKTGFICDS
                     GFNVVASATREGRRLIAVVLGEATGRERATRAASLLEHGFQTHAWKALFAPQSLDSLP
                     MAEDAKGPMTMRQAVVSYECGTARKRRPVAKAPKRKATASAEVVPAEAAKPAKKAPVA
                     AGKASKTDKAAVSAGSRAE"
     gene            complement(4411..5280)
                     /locus_tag="VFR00_12750"
     CDS             complement(4411..5280)
                     /locus_tag="VFR00_12750"
                     /inference="COORDINATES: protein motif:HMM:NF013788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C13 family peptidase"
                     /protein_id="HEU0160183.1"
                     /translation="MSRSSAVLAWQRCLRALIGAAFLWLIPFDPVAGASRPAAEITRT
                     LQLQNELLGRTLKALRPAATGGPHLYFVGFAGYGPQAVFKREVLAVRRFFDARYGTEG
                     RSVALVNHASTLNDLPLASAGNLDRTLQHLGKLMTPSRDVLFLFLTSHGEDGRFVVEM
                     PRFGLASLTPQQLKDMLKRSGIKNSVIVLSACHAGSFLPALADPTTLVIAAARADRSS
                     FGCDDRREWTYFGEAYFDRALRAEPSFVRAFERARELIATWEAKAKLEPSLPQMGGGE
                     RLGPALAAIQEHH"
     gene            complement(5410..6366)
                     /locus_tag="VFR00_12755"
     CDS             complement(5410..6366)
                     /locus_tag="VFR00_12755"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HEU0160184.1"
                     /translation="MDFGVATSTRGATSTREGYIAVAEAAERLGFGFLSVNDHVVVPG
                     GIASKYPYSGDHKWAGASVGECLDLITTLAFLAGRTERLQLLSSVMVVPQRQPVLTAK
                     MLASVDVLSQGRLIVGCGVGWLKEEFEALSAPPYAERGRVTDEFIAAFKVLWTQDRPA
                     FHGRHARFKDLIFAPKPVRKPHPPIWIGGESPLALQRVVRLGDAWYPASNNPQHRLDT
                     AQRVGAGVQELRRVAEAAGRDPASIDVAFLVLWPVTWTAQGTADSERRLLTGSPEAMA
                     ADIGALAAAGVRHLCLTFQAGTLAETLERMQRFAEEVMPLVP"
     gene            complement(6465..8912)
                     /gene="gyrB"
                     /locus_tag="VFR00_12760"
     CDS             complement(6465..8912)
                     /gene="gyrB"
                     /locus_tag="VFR00_12760"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HEU0160185.1"
                     /translation="MSAKPAKKTNGAQSYGAETIKVLKGLDAVRKRPGMYIGDTDDGS
                     GLHHMVYEVVDNAIDEALAGFASEVLVILNPDGSCTVRDNGRGIPTDIHPEEGISAAE
                     VVMTQLHAGGKFGEGEDENPYKVSGGLHGVGVSVVNALSHVLELKIWRDGKEHFVRFR
                     NGVTEAPLKVIGDAVGQRGTEVTFWPSAETFHNVTEFDYTTLEHRLRELAFLNSGVRV
                     VLADERHADKKREEMRYEGGIGEFVRFIDRSKVPLVEAPIMLGAEKDGVGIELALWWN
                     DAYHEQVLCFTNNIPQRDGGTHLAGFRAALTRVINKYAEETGIAKKEKIALTGDDARE
                     GLTAVLSVKVQDPKFSSQTKDKLVSSEVKTVVESVVGDQLAAWFEEHPKDGKIILTKV
                     VEAAAAREAARKARELTRRKGALDINSLPGKLAECQDRDPANTEMFIVEGDSAGGSAK
                     QGRNREFQAVLPLRGKILNVERARFDKMLSSQEIGTLITALGTGIGRDDFDLSKLRYH
                     KIIIMTDADVDGAHIRTLLLTFFYRQMPELVEKGHLYIAQPPLYKVSKGKSETYLKDQ
                     RAFEDYLIDAGLEETTLALGSGEVRAGRDLRDVVEQARAVSKVIEDVHSRYARFVVEQ
                     AAIGGAFEQKLLLSSEAANAKAASIAARLDMQADETERGWSGRLGNEGYVFRREVRGV
                     TEVHMLDRALLGSLEARRLNERQPHLQEIYATPAKLRRKDSETMVFGPRSLLDAVYDA
                     GRKGISMQRYKGLGEMNPEQLWETTLDKETRTLLQVKIGDLGEADEIFSRLMGDIVEP
                     RREFIQSNALAVANLDV"
     gene            complement(9061..>9486)
                     /locus_tag="VFR00_12765"
     CDS             complement(9061..>9486)
                     /locus_tag="VFR00_12765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018859756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication and repair protein RecF"
                     /protein_id="HEU0160186.1"
                     /translation="TRAAVDVEDDYCALLARERELDRAAGRTRSGPHRSDLMAGHGDK
                     EMPAKLCSTGEQKALLIGLILAHADLVSQHRGGAAPLLLLDEITAHLDPSRRLALFAE
                     IQRLGCQAWMTGTDVQAFAGLVEHAQFLDVHDGRVRAGL"
BASE COUNT         1677 a         3164 c         3068 g         1577 t
ORIGIN      
        1 aggcgagctg gcgcaagcga tcgtttgcgc ccacggccgg tgcggccgga actgcaaggg
       61 tggcggccgt catggctggg ggcctcccat cgattaggcc cgcgccgctc caagggtgcg
      121 caggacatcg ctgccaccgc cggacgcgcg aatcaccgga ggctggcaag agcatggaga
      181 agacggcctt ctgagccaag ggctctcgac cgcgccagcg ggcaggccgc gggcagcgcc
      241 agcggcggca tcggcgtgcg ggccacgctt ggcgcgcaaa acctgaaatt ggcccaactc
      301 ctgtctcaat tgcggtgtgg gatcgcggcg ggagacaggg gacaccaatc gagtttcgca
      361 aggggtcgcg cggccttagg gcaggcgagg cgcggaacca ttgcaagcac gggggccctg
      421 taacccgatg tggcagggtt gcgcccccac cgttaacgtc gagttaacgg ctcagcgcga
      481 tccttgtccc acgagtatcc ctgtttggga ggccaagatg tatcgcgtac tgggttcggc
      541 gctcctttcg gctatgttgg ccggctgcgg catggccgcc tcgccgctga cggcgggttt
      601 tgccaactct ggccaaccca cctcctcgct gccgacgcgc gcgcaaggcg ccggcgtcga
      661 cacggcaatg gcgagcgacc cggtcaaacc ggcactcaag gccgtcgata aagcgccgaa
      721 gggaagcttc gaaaaggcgc cgcagggcgc actggccgac cgcgactaca cgaaaaccga
      781 actcgacttg gaaaaggccc gccagctcat caatgcctac cgcaaacaac acggcctgaa
      841 gccgctcacg ctcaacgcgg cgctgacgga agccgccaag ttgcacgcgc gcgacctcgc
      901 caagtgggat cgcatttccc actacggctc ggatggctcc aacccttggg accgggtcaa
      961 gcgcgtcggc tacagggcca aagtggcggc cgaaaacgtt ggcaccggcc aggtcagcat
     1021 cgatgaggtg ctgaagggct gggaggcaag cccgggacac aacaaaaacc tgttgctgcg
     1081 cgacgccgag catatgggca ttgccctggt gcaggaccca cgcaccgagt tcaagacgtt
     1141 ttggacgctg ctggtggggg cgtcgctctg aggcggggag gcaacccctc acgctcgatc
     1201 ctggtcgcgg ctttggccgt gatgacagtg ggcgcctgag gacgagcgct gcggctcgcc
     1261 cgaccctgca ctgctgtctc actcacgcat cccagatcgc cgtcctcacg ttcagacgac
     1321 gccgcgccag cgcgcagttg ccagccgcgg cagcaaatag cgcaaggctc gtcgcggccg
     1381 ccgccccagc cagccccagc accggcacca ggacgacgct cagccccaca ttgagggccg
     1441 ctgccacaag cacgatgcgc gcacaggcgg caccttggcc cagcatgttc agcaccacgt
     1501 cggcgggccc caacgccgca cgcgcgacca ggcctagcgc gaggatgccc atgaccggat
     1561 aggccgctgc aaactgcgga tcaaacaggg caaggatcgc cctgccggcc aacaagatgg
     1621 cgcaggcgcc cgcgagcgat ggccagaaaa tccagtgcgt ggccttggcc atggcgacgc
     1681 cgagccccgc gcggtcgccg cgcgcatcga gggcgacaaa cctgggcgcc atggcgctgc
     1741 cgaccgcgta atgcacaaag agcacgagac ccatggtctt cgcggccgca aagtacatcc
     1801 ctgcctcggc ggggccgagc gaagcggaga ccacgagcac gtcggcgttc tgcagcgtca
     1861 ggtcacaggc caggatcagc cagaagggca gtgccgtctt cagccaatgc gccagcgcat
     1921 agcggcgcgg tccccgcggc aggtcgcgct ggagctgacg gcgcagcaga aggctctgca
     1981 ccatcaccgc gcccaaactc gcgaggatgg ccgcgccaat gccgctgcgt gcggtgaccg
     2041 gaagggcggc agcctgggcg gcggcgagca ggcccaacag tagtaacggt cgcagcacgt
     2101 agggcggtag taggccgagc gccatcagac cgcgcgcgcg tgccatccca tcctggatgt
     2161 cgatcagggt cacgatgggc agacagatga gacccagcag ggccggcacc aggtaggggg
     2221 tcgcgacctg ctcccccaaa cacgccagaa cgccgaagcc gatcagggca atgccgctcg
     2281 cgacggtgac gacgagcagc cgaccggcga aaaagaggcc ccgcaaggcg gcgtcctcac
     2341 cgcgcgcgcg atgctgcgga acgaggcgaa cgacggccgt agaaagccca agattggaga
     2401 gattgcccaa caccaggacc caggtccaaa ccaacacgta aacgccgtag tcgtaggccc
     2461 ccatccagcg cgccagcgcg atctggctga ggtagaggag cccggcactg acgctgcgca
     2521 cgaaaaagac cgtgagcgct tggcgcgcgg cactcccttg gcatcgggcc ggctggacgg
     2581 cggcggggcg cggttcggcc cggcgctcgg tccgcggcca cgcgcgcgcg gccccaaagg
     2641 caaagctggc gggctcagcc actgccttgg ctcctgcgct cactcgcgtt gggcgtgttc
     2701 ttgctggcgc agttcgctgc gatggcgcac ccgatcggcc acattggccc aggcccagct
     2761 gcgcccagca tcgcctttta gcgaatgcaa tgttgagaaa aattcggcaa cgtcggccgc
     2821 ccaaatgcca tggcggcctt cgaggaccgc caccatcgcg tcgatctcca tcggcgtcgg
     2881 cgtcgagtct acgcgagaat gccgccccag gttcatcgtc ctacctcaaa agatggtcgt
     2941 gccggggctg aagcaagttc ggcgccagcc atcccagagc gtttcagccg cgggacaatt
     3001 tcgccgccct tgggttaatc ggcccgccga ttggctctac ggggagcccg ccgctgggac
     3061 gtgcgggccc ggcgtggcgg ccaaatcgca aacggacgtg ccgaactggc acgtcgccgg
     3121 ccagggcgtc ccaattgccg gccggcccca tgggcgcgcg aggccgcggg gcggcgcgcc
     3181 gttgcgccca gtggggccag gggagcgcgc cccggttgtg cgcgccgttc agcgggcggc
     3241 ctattcggcg cggctgccgg cgctcacggc ggccttatcg gtcttggacg ccttgcccgc
     3301 ggccacgggc gccttctttg cgggcttggc ggcctcggcg gggacgacct ccgcactggc
     3361 ggtggccttg cgcttgggcg ccttggcaac gggacgacgc ttgcgggcgg tgccgcattc
     3421 ataagagacc acggcctggc gcatcgtcat cggtcccttg gcgtcctcgg ccatcggcag
     3481 gctgtcgaga ctttgcgggg caaacagcgc cttccaggcg tgcgtctgaa acccgtgctc
     3541 cagcaggctg gcggcgcgcg tagcgcgctc ccgcccggtc gcctcgccga gcaccaccgc
     3601 aatcaggcgc cgaccctccc gcgtggcgct ggccacgacg ttgaagccgc tgtcgcagat
     3661 aaacccggtc ttgaggccat cggcaccttc gtaattggcg agcaacccat tgtgggtgcg
     3721 caggtgccga ttgccgatgc gcacgtccat cagcgcccag agcggcgcat attcagggta
     3781 gtccttgacg acggccacgg acagcttggc aaggtcgcgc gcggtcgtca cctgttcggc
     3841 cgccggcagc ccgttggcgt tgacgaactt ggtgcgcgtc atgccgagcc tggctgcggt
     3901 ggcgttcatc ttctcgacga aggcctcctg ggagcctgca accgcctccg cgagcatcac
     3961 cgcgacgtcg ttggccgact tgacgatcag ggcctgcagc gccgtttcga cggtcatttc
     4021 cgccccaacg ggcagcccaa ccttactggg cggttggctg ttggcgagtt ccgagcagcc
     4081 gatcttgctc tccggcgtga tcttgccggc cttgatggcc tcgaacacca cgtaagccgt
     4141 catgatcttg gtgagcgagg cggggtgcca ctgattgtcc tgatcctcgg cgtaaagcac
     4201 gctacccgtg ccggcatcca acaggagcgc tgggcccgcc aacgcgggca gcgcggccag
     4261 gcccgtggcg agcaccattc ccctcaggat cgtcgccagc atgcctgtcc ctcgatcgtg
     4321 ttgagctcga tcaacgctcc tcgcgcggga tcgttgcgac tcacgtgtcc ccaaggcgat
     4381 ggtcataaac gctgtgctct tgctgcacaa tcaatgatgc tcttggattg cggccagagc
     4441 cggtccgagc ctttcgccac cccccatctg tggcagggaa ggctcaagct tggccttggc
     4501 ctcccacgtc gcgatcagct cacgggcgcg ctcgaaggcg cgcacgaagg atggctcggc
     4561 gcgtagagcc cgatcgaagt aggcttcgcc gaaataggtc cactcacggc ggtcatcgca
     4621 accaaaggag ctgcggtcgg cgcgggcggc tgcgatcacg agcgtggtgg ggtctgcaag
     4681 cgcgggcagg aaactgcccg cgtggcaggc ggacagcacg atcacactgt ttttgatgcc
     4741 cgagcgcttc agcatgtctt tcagctgctg cggggtcagc gaggcaagac caaagcgggg
     4801 catctccacc acgaaccgcc cgtcctcacc gtgcgaggtg agaaacagga acagcacatc
     4861 ccggctggga gtcatgagct tacccagatg ctgcaaggtg cgatccaagt tgccggcgct
     4921 ggcgaggggc aagtcgttga gggtgctcgc atgattgacg agggcgaccg agcgtccctc
     4981 ggtgccgtag cgggcatcga agaacctgcg cacggccagc acctcgcgct tgaatacggc
     5041 ctgcgggcca tagccggcaa agcccacgaa atagaggtgc ggtccccccg ttgccgcggg
     5101 ccgcagcgcc ttgagcgtcc gaccgagcaa ctcattttgc agctgcaagg ttcgcgtgat
     5161 ctcggcggcc ggccgcgagg cgccggcaac cggatcaaag gggataagcc acaggaaggc
     5221 cgcgccgatg agagcgcgca ggcagcgctg ccatgctaag accgcgcttg atcgtgacat
     5281 ccttggcctc atgcatgtcc tttgatcgtt gagctttggc cgcgtcctaa gggcaggtca
     5341 gccacacgct tgcgaggacc tcacggaagg aggcccgcgc tgcgccgcgg ccgccgagga
     5401 gaaggattgc tagggcacga gcggcatgac ctcctcggca aaccgctgca tccgctccag
     5461 ggtctccgcg agcgtgcccg cctggaaggt gaggcacaga tgccgcactc ctgccgcggc
     5521 aagtgcgccg atgtcggcgg ccatggcctc ggggctcccc gtcagcaagc gccgctcgct
     5581 gtcggcggtc ccctgcgcgg tccaggtcac gggccacagc acaaggaagg ccacgtcgat
     5641 gctagccgga tcgcgcccgg cggcctcggc aacgcggcgg agctcttgca cacctgcccc
     5701 aacgcgctgg gccgtatcga gccggtgctg tggattgttg gaggccggat accaggcatc
     5761 accgaggcgt acgacgcgct gcagcgccag cgggctttcc ccgccgatcc aaatgggcgg
     5821 atgcggtttg cgtacaggct tgggagcaaa aatcagatct ttaaagcgcg cgtgcctgcc
     5881 gtgaaaggcc ggtctgtcct gggtccagag caccttgaag gccgcgatga attcgtccgt
     5941 gacgcgtccg cgctcagcgt agggcggggc cgaaagggcc tcgaactctt ccttgagcca
     6001 accgacaccg caacccacga tcagccgccc ctgcgagagg acgtcaacgc tcgccagcat
     6061 tttggccgtc agcacgggct ggcgctgcgg caccaccatc accgagctca acaactgcag
     6121 gcgttccgtg cggccggcca ggaaggccag tgtggtgatg agatcgaggc attccccgac
     6181 gctggcgccc gcccacttat gatcgccgct atagggatat ttcgaggcaa tgccgcccgg
     6241 caccacgaca tgatcattga cgctcaaaaa accgaagccg aggcgctcgg cggcctccgc
     6301 aacggcgata tagccctcac gcgtgcttgt tgctcctcgc gtcgaggtcg ccacgccgaa
     6361 gtccatgcgc tccacctttg tcgccccgcc ggtgcaaatc taggcgcgac cgttatacgg
     6421 ccaaggctgc ctgcttgtca gccgcccgtc gggggcgaaa tcgctcagac gtcaaggttg
     6481 gcaacggcaa gcgcgttact ttggatgaat tcgcgacgcg gctcgacgat gtcgcccatc
     6541 agccgcgaaa agatctcatc ggcttcgccg aggtcgccga tcttcacctg cagcagcgtg
     6601 cgcgtttctt tgtcgagcgt ggtttcccac agctgttcgg ggttcatttc gcccagcccc
     6661 ttgtagcgct gcatgctgat gcctttgcgg cccgcatcat agaccgcatc cagcaggctg
     6721 cgcggcccga acaccatggt ttcgctgtcc ttgcgccgga gcttggccgg cgttgcgtaa
     6781 atctcctgca agtgcggctg gcgctcattc aaccggcgcg cctcgagcga gcccagcagg
     6841 gcccggtcga gcatgtggac ttcggtgacg ccgcgcactt cacgccggaa cacgtagcct
     6901 tcattgccga ggcggccgct ccagccgcgc tcggtttcgt cggcctgcat gtcgaggcgc
     6961 gcggcaatgc tggccgcctt ggcgttggcc gcttcgctcg agagtagcag cttttgctcg
     7021 aacgccccgc cgatggcggc ctgctccacg acgaagcgcg cgtagcgcga atgcacatcc
     7081 tcgatcacct tggagaccgc gcgtgcctgc tcgacgacgt cgcgcagatc gcggccggcg
     7141 cggacctcgc cgctgcctag ggccaaggtt gtctcctcga gcccggcatc gatcaaatag
     7201 tcctcgaagg cccgttgatc cttgaggtag gtctccgatt ttcccttcga gaccttatag
     7261 agcggcggct gggcgatgta gaggtgcccc ttttccacga gctcgggcat ttgccgatag
     7321 aaaaaggtca acagcaacgt gcggatgtgc gcgccatcca cgtcggcgtc cgtcatgatg
     7381 atgatcttgt ggtagcgcag tttggagaga tcgaaatcgt cgcggccgat gccggtgccg
     7441 agcgcggtga tcagcgtgcc gatttcctgg ctggacagca tcttatcgaa ccgcgcccgc
     7501 tcgacgttga ggatcttgcc gcgcaagggc agcaccgcct gaaactcgcg gttgcgcccc
     7561 tgtttggcgg agccaccagc cgagtcaccc tcgacgatga acatttccgt attggcggga
     7621 tcgcgatcct ggcactcggc gagtttgccc ggcagcgaat tgatgtcgag ggcacccttg
     7681 cggcgcgtca gctcgcgcgc tttgcgcgcg gcctcccgcg cagcggcggc ctccaccact
     7741 ttcgtgagaa tgattttgcc gtcttttgga tgctcttcga accaggccgc cagctgatcg
     7801 ccgaccacac tctcgactac ggttttcacc tccgaggaga cgagcttgtc tttggtctgg
     7861 ctcgaaaact tcgggtcctg caccttgacc gacaacaccg ctgtcaagcc ctcgcgcgcg
     7921 tcgtcgccgg tgagagcgat cttctctttt ttggcgatgc cggtttcctc ggcatactta
     7981 ttgatcaccc gcgtcagcgc cgcccgaaac ccggcgaggt gcgtgccgcc gtcgcgctgc
     8041 ggaatgttgt tcgtgaagca gagcacctgc tcgtgatagg catcgttcca ccacagcgcc
     8101 agctcgatgc cgacgccgtc cttctccgcg cccaacatga tgggcgcctc caccaagggc
     8161 accttggaac ggtcgataaa gcgcacgaac tcgccgatgc cgccttcgta gcgcatctcc
     8221 tcgcgcttct tgtccgcgtg gcgctcgtcg gcgagcacca cacgtacccc cgaattgaga
     8281 aaggcgagct cacgcagtcg atgctccagc gtcgtgtagt cgaactcggt cacgttgtgg
     8341 aaggtctcgg ccgagggcca gaaggtcacc tcggtgcccc gctggccgac tgcatcaccg
     8401 atcactttga gcggcgcctc cgtcaccccg ttacggaagc ggacgaaatg ttccttgcca
     8461 tcgcgccaaa tcttgagttc gagcacgtgg gaaagcgcgt tgaccaccga cacgccaacc
     8521 ccgtgcaggc cgcccgatac cttgtagggg ttctcgtcct ccccctcgcc gaatttgccg
     8581 ccggcatgca actgcgtcat gaccacttcg gcggccgaga tgccctcttc cggatggatg
     8641 tcggtcggaa tgccccggcc gttgtcgcgc acggtgcagg atccgtcggg attgaggatt
     8701 accagcacct cgctggcgaa gccggccaag gcctcgtcga tggcattgtc gacgacctcg
     8761 tagaccatgt ggtgcaggcc cgagccatca tcggtatcgc cgatgtacat acccggacgc
     8821 ttgcgcaccg cgtcgaggcc cttcaaaacc ttaatcgtct cggcgccata actttgggca
     8881 ccattggtct ttttggcggg cttcgcgctc atggaagagg gtctcgattg gggggatttt
     8941 tgcttgctcg aactatacac caccacggac ctgagatcac gctaaaaact ccgccaaaat
     9001 gcgcaaaata gttcatgtat ttcaatatat tagcgaggca agaggcgccg cgatggagcc
     9061 ttacaatcca gccctcacgc ggccgtcgtg gacatcaagg aactgggcgt gctcaacgag
     9121 acccgcgaag gcttgaacgt cggtgcctgt catccaggcc tggcagccga ggcgctggat
     9181 ttcggcaaac aacgcaagcc ggcggctggg atcgagatgg gccgtgattt catcgagcag
     9241 gagcagcggg gcggcgccgc cgcgatgctg gctgaccaga tcggcgtgcg ccaggatcaa
     9301 gccgatcagc aaagcctttt gctcgcccgt ggagcaaagt tttgccggca tctccttgtc
     9361 gccgtgcccc gccatcagat cggatcgatg cggaccggaa cgcgttcgac ccgcggcacg
     9421 atcgagctcg cgctcgcgcg ccagcagggc acagtaatcg tcctcaacat cgacggcggc
     9481 ccgcgt
//
LOCUS       DASTMT010000557         5585 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_1961504, whole genome shotgun sequence.
ACCESSION   DASTMT010000557 DASTMT010000000
VERSION     DASTMT010000557.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 5585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5585
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1961504"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..517)
                     /locus_tag="VFR00_12770"
     CDS             complement(<1..517)
                     /locus_tag="VFR00_12770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HEU0160187.1"
                     /translation="MSERASSRPAGLRPARRGAPKRVSVLGATGSVGQSTLDLIGRNP
                     NLFKVVALTARHNIERLAELAVRHGAELAVIAEESQYKALQERLAGTGIATAAGMPGL
                     IEAALRPADCVMAGIMGAAGLRPTLAAVSQGRRVALANKECLVSAGELFMEAVRRAGT
                     ELIPVDSEHSAA"
     gene            complement(543..1415)
                     /locus_tag="VFR00_12775"
     CDS             complement(543..1415)
                     /locus_tag="VFR00_12775"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEU0160188.1"
                     /translation="MSERSEPGGEEGSPRAGLTWLGSDFTTRLVSGLVMVALAAAALI
                     AGPAAFNALVFVLALLLSWEWGRLVHGRSADTVIAVHIAATALAVGLAALGFVGLGLL
                     ALPIGAIVAMLLSLGRHSVFAALGVFYAALPAVILIWLRSDPTYGLRAAVYVILLVIA
                     ADTGAFLAGRGLAGPKLWPSVSPNKTWAGLLGGILAGGLTGALMAFAVPGGSALRLGA
                     TAAVLAFLAQMGDLMESAIKRRFGAKDTSALIPGHGGVMDRVDGLVIAATAAGLAGFF
                     INVHSPARALLLGY"
     gene            complement(1442..2197)
                     /locus_tag="VFR00_12780"
     CDS             complement(1442..2197)
                     /locus_tag="VFR00_12780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HEU0160189.1"
                     /translation="MNEQNKRSVWQASAQVPHHVAIIMDGNGRWASERGLPRSEGHRR
                     GVEAVRRTVRAAMELGIQYLTIYSFSSENWSRPASEVDDLMGLMKRFIRRDLAKLHRN
                     GVRISVIGERTGVEPELLGLMDEAVALTSRNSALNLMIAFNYGSRTEIAKAARRLAGR
                     VLAGSINLEDITPETLAGELDTQGVPDPDLLIRTSGELRLSNFLLWQSAYTEFVFLDA
                     YWPEFGRELLGRAIDEFRGRQRRFGGVATQSTG"
     gene            complement(2294..2857)
                     /gene="frr"
                     /locus_tag="VFR00_12785"
     CDS             complement(2294..2857)
                     /gene="frr"
                     /locus_tag="VFR00_12785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012112125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEU0160190.1"
                     /translation="MAAGKFEIQELERRMRGALESLKKEFAGLRTGRASVHFLDPVVV
                     NVYGQRMPLNQVATVSTPDARTISVQVWDRGQVSAVEKAIREANLGLNPVVDGALIRL
                     PIPALTTERRQELVKVAHKYAEHARVAVRNVRREGMDLLKRLDKDGQMSEDDHHKNSS
                     KVQDLTDKLIKEVDQSLASKEAEIKQV"
     gene            complement(2931..3668)
                     /gene="pyrH"
                     /locus_tag="VFR00_12790"
     CDS             complement(2931..3668)
                     /gene="pyrH"
                     /locus_tag="VFR00_12790"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEU0160191.1"
                     /translation="MAAASSSPLRYRRVLLKLSGEALMGAGGYGIDMRVAQRLAQDIK
                     EAIAAGAEIAAVVGGGNIFRGLAGVAKGMDRATGDYMGMLATVMNALALQNALEQAGV
                     QARVLSAIPMPTICESFVRSKALHHLENGRVVIFAAGTGNPYFTTDTTAALRAVEMNC
                     EVLAKATQVDGVYSADPKLDPKAVRHERLTYAEVLSRDLKVMDGAAIALARDNNLPVI
                     VFSIEEPGNLSKLLRGEALSTLVSGAA"
     gene            complement(3835..4773)
                     /gene="tsf"
                     /locus_tag="VFR00_12795"
     CDS             complement(3835..4773)
                     /gene="tsf"
                     /locus_tag="VFR00_12795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013948487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HEU0160192.1"
                     /translation="MTAISAGQVKQLRDKTGAGMMDCKTALTETKGDMEQAVDWLRKK
                     GLSKAATKAGRVAAEGLIGVTVSDQTGAIVEVNSETDFVARNEKFQDMVRKVAVGAAK
                     ANGDLNKLLGTPVPGSALSVEDYVKEMVATIGENMSVRRTASLSVAQGAVADYVHNKA
                     ADGLGKIGVLVGLESKAEPKAVAALGRQLAMHIAAASPAAVTAEELDPALIARERAIY
                     TEQATASGKSADIVGKMVEGRLRKEFYQQVVLLQQTFLGAGGDGKQTVAQVLEEATKR
                     LGAPVKVHAFVRYALGEGIDKKDDDFAAEVARAAGR"
     gene            complement(4848..>5585)
                     /gene="rpsB"
                     /locus_tag="VFR00_12800"
     CDS             complement(4848..>5585)
                     /gene="rpsB"
                     /locus_tag="VFR00_12800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012334544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEU0160193.1"
                     /translation="CAQYYINHRWLGGTLTNWKTISQSIRRLKLLDEMLAGEVKGRTK
                     KEVLQLTRDRDSLNRSLGGIKEMGGLPDLLFVIDTNKEQIAIAEAKKLGIPIVAIVDT
                     NCDPDGIDFVVPGNDDAGRAITLYCDLVARAAIDGIERGQTQAGVDVGAAEAPPAEDL
                     AAEKAPLFKGIDAPRGEADDLKRLPNVNPRLEQRLNDSGVYHFWQIADLDEQSMAGLD
                     RLLNLKGRIERDGWVAAAKQLVEGQAA"
BASE COUNT          947 a         1906 c         1751 g          981 t
ORIGIN      
        1 aggcggcaga atgctctgag tcgacgggaa tgagctcggt gccggcgcgc cgcacggcct
       61 ccatgaaaag ctcacctgcc gacaccaggc attccttgtt ggccagcgcg acgcggcggc
      121 cctgcgacac ggccgccagg gtcgggcgca ggccggccgc acccatgatg ccggccatca
      181 cacaatcggc gggcctgagc gccgcctcga tcaaccccgg catgccggcc gccgtggcga
      241 tgccggtgcc ggccagacgc tcctgcaagg ccttgtattg gctttcctcc gcgatcacgg
      301 cgagctcggc gccatgcctg acggccagct ctgccagacg ctctatattg tgccgcgcgg
      361 tcagggcgac aaccttgaag agattgggat ttcggccaat cagatcgagc gtgctctgtc
      421 ccacggagcc cgtcgcgccg agcacgctga cgcgctttgg cgcgccgcgg cgcgccggcc
      481 tgaggcctgc cggccggctg gaggctcgct cgctcatcgg caccgccgct cttgcgccca
      541 tcctaatacc ctagcagcag cgcgcgtgcc ggcgaatgca cattgatgaa aaagccggcc
      601 aggcctgccg ccgtcgccgc gatcaccaga ccatccaccc gatccatgac gccaccatgg
      661 cccggaatca gcgcgctggt atccttggca ccaaaccggc gcttaatggc cgattccata
      721 agatctccca tctgcgccag aaaggcgagg acggccgccg tggccccgag ccgcagtgcg
      781 gagccgcccg gcacggcgaa cgccatgagg gcgccggtga ggccacccgc caggatgccg
      841 ccgaggaggc ctgcccaggt cttgttgggc gaaacgctag gccacagctt cgggcccgcc
      901 aggccgcgcc cggcgagaaa ggccccggta tccgcggcga tgacgagcag gatgacgtag
      961 acggccgccc gcaggccata ggtgggatcg gatcgcaacc atatcaggat aactgccggt
     1021 agagccgcat aaaacacccc aagcgcggca aaaacactgt ggcggccgag gcttaacagc
     1081 atggccacga tcgccccgat cggcagggcg agcaggccaa ggcccacgaa gccgagcgcg
     1141 gcgaggccga cggcgagcgc ggtcgcagcg atatgaacgg cgatcacggt gtccgcgctg
     1201 cggccatgca ccaagcggcc ccactcccag ctcaacagca gcgccagcac aaaaaccagg
     1261 gcgttgaaag cggccgggcc ggcgatgagg gcggcggcgg cgagcgccac catgaccagg
     1321 cctgagacaa ggcgggtggt aaaatcgctg ccgagccacg tgaggcctgc gcggggggat
     1381 ccctcctcac cacccggctc gctgcgctcg ctcatcggca tggccccctt gcctgccaac
     1441 cctagcccgt cgactgcgtc gccaccccac cgaagcggcg ctgccgtcca cggaattcgt
     1501 cgatggcgcg ccccaagagc tcgcggccga actcgggcca ataggcgtcg aggaacacga
     1561 actcggtata ggcggactgc caaagcagga aattggagag ccgcagctca ccgctggtgc
     1621 ggatcaacag gtcggggtcg ggaacgcctt gcgtgtcgag ctcgccggcc agggtctccg
     1681 gtgtgatgtc ctcaaggttg atgctgcccg cgagtacccg gcccgccagt cggcgcgccg
     1741 ccttggcgat ctcggtgcgc gatccgtagt tgaaggcgat catcaagttg agcgcgctgt
     1801 tgcgcgaggt gagcgccacg gcttcgtcca tcagccccaa cagctcgggc tcgacgccgg
     1861 tgcgctcgcc gatgacgctg atgcgcacgc cgttgcgatg cagctttgcc aggtcgcggc
     1921 ggatgaagcg cttcatcagc cccatcaggt cgtcgacctc actggcggga cgcgaccagt
     1981 tctccgagga aaagctgtag atcgtcaggt attggatgcc gagctccatg gccgcgcgca
     2041 cggtccggcg taccgcctcc acgccgcgcc ggtgcccttc cgaacgtggc aggccgcgct
     2101 cggaagccca gcggccattg ccatccatga tgatggcgac gtggtgcggc acctgcgcac
     2161 tcgcctgcca gaccgatcgt ttgttctgct cgttcatgga tcaggcatca tgcccagcat
     2221 cgcgtggcga cggcgcccat cttagcgctc gatttgggcg cccaactatt ccgcgctccg
     2281 cggtccccaa ccatcatacc tgcttgatct cggcttcttt gctcgccagc gactggtcga
     2341 cctccttgat gagcttgtcg gtcaaatcct gcaccttgct tgaattcttg tgatgatcgt
     2401 cctcgctcat ctgaccatcc ttgtcgaggc gcttgagcag gtccatgccc tcgcgacgca
     2461 cgttgcgcac cgccacgcgc gcatgctcgg catacttgtg cgcgaccttg acgagctcct
     2521 ggcgccgctc ggtggtcagc gcggggatcg gcagcctgat caaagccccg tcgacgacag
     2581 ggttgagacc gaggttcgcc tcgcggatgg ccttttccac ggcgctgacc tggccccggt
     2641 cccaaacctg cacggaaatc gtgcgcgcgt cgggggtgga caccgtcgcc acctggttca
     2701 gtggcatgcg ctggccgtag acgttcacca ccacagggtc gaggaaatgc acgctcgccc
     2761 ggccggtgcg caggccggcg aactcctttt tcagggactc cagggctccg cgcatgcggc
     2821 gttcaagttc ttggatttcg aattttccgg cagccatgac acatttctcc gcttgggctg
     2881 tcccgcaacg agcctgcggc gcagccttca accaccgtcc accaagccat ttatgcggcg
     2941 cccgagacca gcgtggatag ggcctcgccg cgcaggagtt tgctgagatt accgggttct
     3001 tcgatggaaa acacaattac cggaagatta ttgtcgcgag caagcgcgat ggcggcccca
     3061 tccatgacct tgaggtcccg gctcagcacc tcagcatagg tcagacgctc gtggcgcacg
     3121 gccttgggat cgagcttcgg atcggccgaa tagaccccgt cgacttgcgt ggccttggcc
     3181 aagacctcgc agttcatctc caccgcgcgt agcgcggccg tggtgtcggt ggtgaagtag
     3241 ggattgccgg tgccggcagc gaaaatgacg acgcggccat tttctagatg atgcaaggct
     3301 ttggagcgca cgaaggactc gcagatggtc ggcatgggaa tggcggaaag aacgcgcgcc
     3361 tgcacgccgg cctgctcgag ggcattttgc agcgccagcg cattcatcac ggtggcgagc
     3421 atacccatgt agtcgccggt cgcccgatcc atccccttgg cgacgccggc aaggccgcga
     3481 aagatgttgc cgccgccgac cacggcggcg atttccgcac ccgcggcgat cgcctccttg
     3541 atatcctggg ccaggcgctg cgcaacgcgc atatcgatgc cgtaacctcc cgcgcccatc
     3601 agcgcttcgc cggaaagctt cagcagcacg cgtcggtagc gcaaaggaga ggacgaggcg
     3661 gcggccatca caccagttcc ctcgacataa gagcggcacc gcgcagcggc gttgccggcg
     3721 gaccacggcg ggccggcagc cgtctgcgct tcaacacata accagagcag cccggcgtgc
     3781 gcaaattgcg gccgtcgggc caccccggtt cggtcaaatt atgcctcgtc ttgcctaacg
     3841 gccggcggcc ctggccactt cggcggcgaa gtcgtcatcc tttttgtcga tgccctcgcc
     3901 cagggcatag cgcacgaagg catggacttt gaccggcgct cccagccgct ttgtggcctc
     3961 ttcgaggacc tgtgcgaccg tctgcttgcc atcgccgccg gcgccgagga aggtctgctg
     4021 caaaagcacg acctgctgat agaactcctt gcgcagccgg ccctccacca ttttgccgac
     4081 gatgtcggcc gacttgccgc tggcggttgc ctgctcggta tagatggcgc gttcgcgcgc
     4141 aatcagcgcc ggatcgagct cctcggccgt caccgccgcc gggcttgccg cggcaatgtg
     4201 catggccagt tgccggccca gcgccgccac cgcctttggc tcggccttac tctcgagccc
     4261 gaccagcacg ccgatcttgc cgaggccatc ggcggccttg ttgtgcacgt agtcggcgac
     4321 cgcgccttgc gccaccgaca ggctcgcggt gcggcgcacc gacatgttct cgccgatggt
     4381 ggcgaccatc tccttgacgt aatcctcgac gctgagggcg gaacccggga cgggggtgcc
     4441 caacagcttg ttaagatcgc cgttggcctt ggccgctccg acggcgacct tgcgcaccat
     4501 gtcctggaac ttttcgttgc gcgcgacaaa atcggtctcg gaattgacct ccacgatcgc
     4561 tccggtctgg tcgctgacgg tgacgccgat cagcccctcg gcggcaacgc ggccggcctt
     4621 ggtcgcggcc ttggagaggc ctttcttgcg caaccagtcg acggcttgct ccatgtcgcc
     4681 cttggtctcg gttagggcgg tcttgcaatc catcatgccg gcgccggtct tgtcgcgcag
     4741 ctgtttgact tggcctgcgg aaatagctgt catacgttcc tcgcttgcgc ctcacgcgcg
     4801 ccgttggcgc gctgggaggc tgataactgg agactaaagg ccgcttttta ggccgcctga
     4861 ccttcgacca attgcttggc agccgcgacc caaccgtcgc gctcgatgcg ccccttcaga
     4921 ttgagcaagc ggtcgagccc ggccatcgac tgctcgtcga gatcggcgat ctgccagaag
     4981 tggtagacgc cactgtcatt gagccgttgc tccagccggg gattgacgtt tggcagccgc
     5041 ttgaggtcgt cggcttcgcc gcgcggggca tcgataccct taaatagggg tgctttctcg
     5101 gccgccaaat cctcagccgg cggcgcttcc gccgcaccga catcgacgcc agcctgcgtt
     5161 tgcccacgct cgatgccatc gatggcggca cgcgccacca aatcgcaata gagcgtaatc
     5221 gcccggcccg catcatcgtt gcccggcacc acgaaatcga tgccgtcggg atcgcaattg
     5281 gtgtcgacga tagcgacgat ggggatgccg agctttttcg cttcggcgat cgcaatctgc
     5341 tccttgttgg tgtcgatcac gaacaacagg tccggcagcc cgcccatttc cttgatgccg
     5401 ccgagcgagc ggttgagcga gtcgcggtca cgcgtcagtt gcagcacctc ctttttggtg
     5461 cgccccttga cctcgcccgc cagcatctca tccaacagct tcaggcgcct gatggactgc
     5521 gaaatcgtct tccagttggt cagtgtgccg cccaaccagc gatggttaat atagtattgc
     5581 gcgca
//
LOCUS       DASTMT010000558         1505 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_1961586, whole genome shotgun sequence.
ACCESSION   DASTMT010000558 DASTMT010000000
VERSION     DASTMT010000558.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 1505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1505
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1961586"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(88..1050)
                     /locus_tag="VFR00_12805"
     CDS             complement(88..1050)
                     /locus_tag="VFR00_12805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU0160194.1"
                     /translation="MAVLIEARDLRKRFGAINAVDGISLEVVKGEVLGFLGPNGAGKS
                     TTMKMLTGFLEPDSGSAYIGGINIADQPIAAKAKLGYMPEGAPAYGDMNTVGFLRFIA
                     EVRGFDGAEAERRVQAAVEKAALEAVLEQKIDTLSKGYKRRVGMAQAILHDPQVLIMD
                     EPTDGLDPNQKHHVRKLIADMAKDKAIIVSTHILEEVEAVCSRAVIINKGKIVADGTA
                     ESLMRRLSYHGTIAMHVPSSRAEAVKKALLEFAAIQSVETVGAGNGKVRLQAAPRDGA
                     PAAPQLASLIRTRLIEVEEVVVQRGSLDEVFREITTSDDGAHHG"
BASE COUNT          245 a          475 c          485 g          300 t
ORIGIN      
        1 aagatgatga gaaacaccgt ggccagcggc gttgagaaat aggcggcaaa ttcccgcttg
       61 gtgatggccc aggttttgcg aaagacctca gccatgatgt gctccatcgt cggaggtggt
      121 gatttcgcgg aagacttcgt cgaggctgcc gcgctgcacc acgacttctt ctacctcgat
      181 caggcgcgtg cggatgaggg aagccagctg cggtgcggcg ggagcgccgt cgcgcggggc
      241 ggcctgcagg cgaaccttgc cgttgccggc gccgaccgtc tcgacgcttt gaatggcggc
      301 gaactcgagc aacgcctttt tcacggcctc ggcgcggctc gacggcacgt gcatggcgat
      361 ggtgccgtga taggacaggc gacgcatcaa ggattcggcg gtgccatcgg cgacgatctt
      421 gcccttgttg atgatgacgg cgcgcgagca gacggcctcc acctcttcga gaatgtgcgt
      481 ggagacgatg atggccttgt ccttggccat atcggcgatg agtttgcgca catgatgctt
      541 ttggttggga tcgagcccat cggtcggctc gtccatgatc agcacttgcg gatcgtgcag
      601 aatggcctgc gccatgccga cccggcgctt gtagcccttg gacagggtat cgatcttctg
      661 ctcgagcacg gcttcgaggg cagccttttc caccgccgcc tgcacgcggc gctcggcctc
      721 ggcgccatcg aaaccgcgca cctcggcgat gaagcgcaaa aagcccacgg tattcatgtc
      781 gccgtaggcc ggcgcgcctt cgggcatgta gcccagtttg gccttggccg cgatcggctg
      841 gtcggcgatg ttgatgccgc cgatgtaggc gctgccggaa tcgggctcca ggaagccggt
      901 cagcattttc atggtcgtgg atttgccggc gccgttggga ccgagaaagc ccagcacctc
      961 gcctttcacg acctcgagtg agatgccgtc gaccgcgttg attgcaccaa agcgtttgcg
     1021 caaatcgcgc gcctcaatca ggaccgccat cagcttcact ccccagtatg gtgcggaccg
     1081 ccaccccaca tttttctaaa acggcttgcg cgtggccgat agctcgacag tttggctaga
     1141 aatgggtgtg gcagccatcc aggcgggcac agcccccgac gcgctgcttg ccgaccttga
     1201 ccagactctg ctcccttctg tcaagaggtt gaatggtcac cctttctggg cttgacggcg
     1261 gcacgctggc gcatgcagcg ttcggccggt cgaacgcgag cgggcggcgt cccatcgtcc
     1321 cgtgccgggc gaaggtgggc accgcctggt acgacacgtt gcctcagcgt cggccggcct
     1381 gtggcatctt tgcgggcccg agggcgagcg attgcgccga tgccgccgcc atctcagcca
     1441 cggagccacc ctcgggtcgt cctttgccgc cgtttgccac gcattgccac cctttgccac
     1501 tcttg
//
LOCUS       DASTMT010000559         3327 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_5411755, whole genome shotgun sequence.
ACCESSION   DASTMT010000559 DASTMT010000000
VERSION     DASTMT010000559.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3327
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5411755"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            402..1196
                     /locus_tag="VFR00_12810"
     CDS             402..1196
                     /locus_tag="VFR00_12810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0160195.1"
                     /translation="MRFVFFSSLSAVILLLASLALLVLGRRIGERRLQADPGGAFAGH
                     AAVETAVFGLLGLLLAFTFSGAMKRFDDRRQLIGDEAAALTAAYWQADLLQPAPRALL
                     RDRLRQYLQARLEASRQPRLIEGGQEVFTSDVMKRSEALQAEIWRTASDPTHAIRGES
                     VDHVLLSTLTSLFGAAKARNAAGQRHPPEIIFFMLFGLTVVSALLAGYGMAGSKRPSW
                     VHMAGFALSLAGTVFVIHDIEFPRSGLIRVDQFDQVLVNALRHTPE"
     gene            1416..2522
                     /locus_tag="VFR00_12815"
     CDS             1416..2522
                     /locus_tag="VFR00_12815"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007601107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU0160196.1"
                     /translation="MRVVICGGGVIGASIAYFLSQRGARPIVVERTGIACAASGKSGG
                     FLALDWCDGTPLADLARRSFHLHEHLAQSIERDWGYRRLTTYGGHVTARATRAAADRG
                     DAPTWLSTAVNLGHRLGSPQTTAQVNPGPFAAALMAAATAQGGELRLGTVRGLVRDGA
                     HLQGVDVDGEVIAADAVIIAMGPWSVLAAQWLPLPPVFGLKGHSLIFETGAAVPAEAL
                     FLEYEERGGGVQSPEVFPRADGTTYVCAISSEGPLPLDPAAVGPDPGAIERLEAICRD
                     LSPVLAKAPIIARQACFRPVTEDGLPLIGPVPDLDRVYVATGHSVWGILNAPATGEAL
                     ADLLLDGAAGHVDLAAFDPGRLPALDPKRLKTHA"
     gene            2515..3003
                     /locus_tag="VFR00_12820"
     CDS             2515..3003
                     /locus_tag="VFR00_12820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014332529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin"
                     /protein_id="HEU0160197.1"
                     /translation="MPELLRLGPNGWMPNNPRLPVLLYRQAVDATGADPAQAVEEMFQ
                     RNGWPPQWRNGVYPFHHYHSTAHEVLGFAAGRARLILGGEGGQEVEVAAGDVALLPAG
                     TGHFRRSASRDFLVVGAYPPGQVADLLRSAASPRVLAAIAGVPKPATDPVHGRPLSDF
                     WD"
     gene            complement(3102..>3327)
                     /locus_tag="VFR00_12825"
     CDS             complement(3102..>3327)
                     /locus_tag="VFR00_12825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008126382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEU0160198.1"
                     /translation="FSKLTQYGYSGWASLEWECCLKHPEDGARDGAQFIRDHIIRVQT
                     RAFDVGMMGASDPGRSRRILGLQKTQDGEA"
BASE COUNT          492 a         1147 c         1073 g          615 t
ORIGIN      
        1 acgacgtttg cagggtgtct tgtccaacgt cgctctggac ggacagtact gcagtggtat
       61 ctcccaccct ctgcagggct cccgtgcggc tcgccctctc tgctcgcaag gcgaggtcgg
      121 cgccggcgcg cggctcccgc gtgctctggc ctgccggagc cgatcgaggc gatcccgtgt
      181 agcccacaat gctgccaagg gctcggccga caaacgcagt ccgccttgcg gcacgctcgg
      241 aggccgcgca acacacccag ccaaatatac agataaggcc ctattcccat ttggccgccg
      301 cacccgagca tactccgccg cggcgcgggt tgatgttctt taccgtcagg gaggggttgg
      361 ccggcgacgc cgtcaatctt tccaggacat tgggatcccg catgcgcttt gtgttttttt
      421 cgagcttgtc cgccgtcatc ctcctgttgg cctccttagc gctgttggtg ctgggccgcc
      481 gcatcggtga gcgccgcctg caggcagatc ccggtggcgc ctttgccgga catgccgccg
      541 tcgaaacggc ggtgttcggt ctcctcggcc tgttgttggc gttcaccttc tcgggcgcca
      601 tgaaacgttt cgatgatcgg cggcaactca tcggcgacga ggccgcggcg ctgaccgcag
      661 cctattggca ggcggatctg ctgcagccgg cgccgcgcgc gctgctgcgc gatcgcttgc
      721 gccaatattt gcaggcccgc ctcgaggcct cccgacagcc gcgcctgatc gagggcggcc
      781 aggaagtgtt cacctccgac gtcatgaaac gcagcgaggc tctgcaagcc gaaatctggc
      841 gcacggcgag cgatcccacg catgcaatca ggggcgaatc tgtcgaccac gtgctgctgt
      901 ccacccttac gagtttgttc ggggcggcca aggcccgcaa tgccgccgga cagcgccatc
      961 cccccgaaat catctttttc atgctgttcg gcctcacggt ggtgagtgct cttctcgccg
     1021 gctatggcat ggccggcagc aaaaggccga gttgggtgca catggcgggt tttgcgctca
     1081 gcctagccgg cacggtgttc gtgattcatg acatcgaatt cccccgctcg ggtctgattc
     1141 gtgtcgacca gttcgatcag gtgctcgtca atgccttgcg tcacacgccg gagtaagtcg
     1201 cttgcaccca ttcggcacac ccacgctcag ggtcagtgtc gctgtctccg ccgcgcaaaa
     1261 ccgtaaaggc ctggggtcgc aacacctccg cgtgctaagc tcgccgcgtt tcgcgctgcc
     1321 taggttagga cccatggctc cagagcatgc cttgcccatg aaccccgtgg cggtctcgac
     1381 cgttgcgctc ggcacaggct aaacgagggc gcggcatgcg ggtcgtcatc tgcggggggg
     1441 gtgtgatcgg ggcctcgatc gcctactttc tgagccagcg tggtgcccga cccattgtcg
     1501 tcgagcgcac aggcatcgcc tgcgccgcct cgggaaagtc cggtggcttc ctggcgctcg
     1561 actggtgtga tggaacgccg ctggccgacc tggcgcggcg cagctttcac ctgcacgaac
     1621 acctcgccca gagcatcgag cgcgattggg gctaccgtcg cctcaccacc tatggcggcc
     1681 acgtcacggc gcgggcgacc cgtgccgcag ccgaccgtgg cgatgctccc acctggctat
     1741 cgacggccgt caacctcggc catcgcctgg gttcgccgca aaccaccgcg caggtcaatc
     1801 ccggcccctt cgccgccgcc ctgatggcgg ccgcgaccgc gcaagggggc gagctccgcc
     1861 tcggcaccgt ccgcggcctg gttcgcgatg gcgcgcacct gcaaggcgtc gacgtcgacg
     1921 gcgaggtgat cgccgccgac gccgtgatca tcgccatggg gccctggtcg gtgctggctg
     1981 cgcaatggtt gcccctgccg ccggtgttcg gcctcaaggg acatagtctg atcttcgaga
     2041 ccggtgcggc cgtaccggcg gaggcgctgt tcctcgaata tgaggagcgc ggcggaggtg
     2101 tgcagtcgcc ggaggtgttc ccacgcgccg acggcacgac ctatgtttgc gctatctcga
     2161 gtgagggccc gctgccactc gaccctgccg ccgtcggtcc agatcccggc gccatcgagc
     2221 gcctggaagc gatctgccgc gacctgtcgc cggtgcttgc caaggctccc atcattgccc
     2281 gccaagcctg cttccgaccc gtaaccgagg atggtctgcc gctgatcggt ccggtgccgg
     2341 atctcgatcg cgtttacgtg gcgaccggcc atagcgtctg gggcattctc aacgcgccgg
     2401 cgaccgggga ggccttggcc gacctgctgc tcgacggtgc ggcgggccat gtcgacctcg
     2461 ctgcgttcga ccccggccgc ctgccggccc tcgatcccaa acggctcaag acccatgcct
     2521 gaactcctgc gacttggccc taacggttgg atgcccaata acccgcgctt gccggttttg
     2581 ctttatcgac aggcagtgga cgctacgggc gcggatcccg cgcaagccgt cgaggagatg
     2641 ttccaacgca acggctggcc accgcaatgg cgcaacggcg tctatccgtt ccatcactat
     2701 cattcgaccg cgcacgaggt gctgggcttc gcggccgggc gggcgcgcct cattctcggc
     2761 ggtgagggcg gtcaggaggt cgaggttgcg gcaggagacg tcgcgttgct gccggccggc
     2821 accggccact tccgccgctc ggcgagccgc gacttcctgg tggtcggtgc ctatccgccc
     2881 gggcaagtcg cagacctgtt gcgatcggcg gcgagcccgc gcgtgctcgc cgcgattgcc
     2941 ggcgtaccca aacctgccac ggacccggtg cacgggcggc ccctgagcga cttttgggat
     3001 tgagaggaag ctattgagcg cgactcgcga cccgcatgcg ccgtgggtag cgctgacgtg
     3061 ggcgcgtcct caggcgcgcg ccggcgaccc atcacgcttg cctacgcctc accgtcttgc
     3121 gtcttctgca agccgaggat tcgacgacta cggccagggt cgctcgcccc catcatgccc
     3181 acatcaaacg cacgcgtctg cacgcgaatg atgtggtcgc gaatgaactg ggcgccgtca
     3241 cgggcgccgt cctcgggatg cttgaggcaa cattcccatt ccagtgatgc ccagccgctg
     3301 tagccatact gggtgagctt ggagaag
//
LOCUS       DASTMT010000560         3423 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_3602384, whole genome shotgun sequence.
ACCESSION   DASTMT010000560 DASTMT010000000
VERSION     DASTMT010000560.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3423)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3423)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3423
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3602384"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1110)
                     /locus_tag="VFR00_12830"
     CDS             complement(<1..1110)
                     /locus_tag="VFR00_12830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006612616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEU0160199.1"
                     /translation="MVSIVPAAIGAPTLRIEGRAKVTGSARYSSDEAVPNAAHAYLVT
                     SAVALGRVNGFELEAARRVPGFIDILTHETVRGQVKPPAAPGGKGKTTTTLEADRIWH
                     AGQIIAVVVAETQEAAREAAHKVKVTYAEEAPAASFDSPGVMSELAASANKQQEDPQV
                     GNAAEAFAAAPVKIEADYETPTQHHNPMELFTTTCLWSGDQLTIYEPSQFVHSVRAEV
                     AKQLDLKPENVRVISRLVGGGFGSRGGITSRTAWIALAARRLNRPVKLVATRAQGFTI
                     ATYRAETRHRVRLAAASDGRLQSLSHEGWEVTSRPSTYNVAGTASTARLYNCSNVWTR
                     VNIVHADRSTPGFMRAPPETPYLFALESAMDELAVA"
     gene            complement(1113..2102)
                     /locus_tag="VFR00_12835"
     CDS             complement(1113..2102)
                     /locus_tag="VFR00_12835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008972296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HEU0160200.1"
                     /translation="MRPFAYVSADTPKRAIAASTAESQFLAGGTTLLDLMKLDVLQPE
                     RIVDINPLQARHGGIEKTAEGVRLGALVRMGEAADDPVLRREYPVLAQALQQAASPQL
                     RNMATLAGNVLQRTRCTYFRNPSYHACNKREPGSGCAALDGLNRKHAVLGVSEHCIAT
                     YAGDFAQALIALDASVDVAGRSGERRIPFAQLHRLPANTPDVETTLAPAELITGFFVP
                     SGPWTRRSHYLKIRDRSSFAFALASAAVALHVENGEVREARIGLGGVATVPWRGREAE
                     AVLKGKVLSQETAQAAAEAAFAAAQSRDQNAYKPELGRRTLVRALMTAAALEI"
     gene            complement(2106..2699)
                     /locus_tag="VFR00_12840"
     CDS             complement(2106..2699)
                     /locus_tag="VFR00_12840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012706539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEU0160201.1"
                     /translation="MADGTVNRRGFLSATAVAAASGLVPAAATAQGSEGPGGVAGDKH
                     SVSLSVNGRPHELVIDPRRTLLDLLRDDLALTGAKKGCDHGQCGACTVLVGGKRVLAC
                     LTLAAAVRAEVSTIEGLASADGALHPMQEAFMQEDAFQCGYCTPGQILSAIACVREKH
                     ANSEDEIREFMSGNICRCAAYPNIVRAIARAKSRTEA"
     gene            complement(2925..>3423)
                     /locus_tag="VFR00_12845"
     CDS             complement(2925..>3423)
                     /locus_tag="VFR00_12845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEU0160202.1"
                     /translation="LPEKMTGGLKNPLGAMALYLGDTLYRIHGTNDVKSIGQAQSSGC
                     FRMLNTAVVHLASMAEVGTPVSVVEHLPARQELSRAAPPRPAPPAGEAQPSPLRPAPP
                     AGEAQSSPTVPAPPAGEAQSSPAIPAPPAGEAQSSPAIPAPSRTGTEALPDYSSLRDY
                     ALQRR"
BASE COUNT          542 a         1127 c         1174 g          580 t
ORIGIN      
        1 cgcaaccgcc agctcatcca tcgcgctctc cagcgcgaac agatagggcg tttccggcgg
       61 cgcgcgcatg aaccctgggg tgctgcgatc agcatgcaca atattgaccc gggtccaaac
      121 gttgctgcag ttgtagagcc gagccgtgct cgcggtgccc gcaacgttgt aggtcgaggg
      181 tcgcgacgtg acctcccagc cttcatggct caagctttgc agccggccgt cggacgccgc
      241 agccaggcgc acacggtggc gcgtttcggc gcggtaggtg gcgatggtga acccctgcgc
      301 gcgcgtcgcc accagtttca ccgggcgatt gaggcggcgc gccgccaagg caatccaggc
      361 ggtccgcgag gtgatgccgc cgcgcgaacc aaagccgccg ccgaccaaac gcgagatcac
      421 gcgcacgttt tcgggcttga ggtccagttg cttggcaacc tccgcccgta ccgaatgcac
      481 gaactggcta ggctcgtaga tggtgagctg gtcgccggac cataggcacg tcgtggtaaa
      541 aagctccatc gggttgtggt gctgggtcgg cgtttcgtag tctgcctcga tttttactgg
      601 cgccgccgcg aaagcttccg cggcgttgcc gacctgcggg tcttcctgct gcttattcgc
      661 gcttgccgcg agctcgctca tcactccggg gctgtcgaag ctggcggcgg gtgcctcctc
      721 ggcgtaggtg accttcacct tatgggccgc ctcgcgcgcc gcctcttgag tttccgccac
      781 caccacggca atgatctggc cggcgtgcca aatgcgatcc gcttcgagcg tggtcgtggt
      841 cttacccttg ccgccgggtg ccgccggcgg cttgacctgc ccccgcacgg tttcatgcgt
      901 gaggatgtca atgaacccag gcacgcggcg cgccgcctcc agctcgaagc cattaacgcg
      961 tcccagcgcg acggcgctgg tgacgaggta ggcgtgcgcc gcattcggca ccgcctcatc
     1021 ggaactatag cgggcgctgc cggtgacctt cgcccggccc tcgatgcgca aggtcggggc
     1081 gccaatcgcg gcaggcacga tactgaccat gctcaaatct ccagggctgc ggccgtcatc
     1141 agcgcccgca ccagggtgcg gcgtccgagc tccggcttgt aggcgttctg atcgcggctc
     1201 tgcgccgcgg caaaggcggc ttccgccgcc gcctgggcgg tctcctggga cagcaccttg
     1261 cccttgagca cggcttcggc ttcccgtccc cgccagggca cggtggcaac gccgcccaag
     1321 ccgatgcgcg cctcgcgcac ctcaccgttc tccacatgca gggccaccgc ggcggaggcg
     1381 agcgcaaaag cgaaggagct acgatcgcga attttgaggt agtgcgagcg ccgggtccac
     1441 ggcccgctcg gcacgaaaaa gcctgtaatc aactcggcgg gggccagcgt cgtttccacg
     1501 tctggcgtat tggcaggcag gcggtgcagc tgagcgaagg ggatgcggcg ttcaccgctg
     1561 cggccggcga catcgacgct agcgtcaagc gcgatcagcg cctgggcgaa atcacctgcg
     1621 taggtggcga tgcagtgctc gctgacgccg agcacggcgt gcttgcggtt gaggccgtca
     1681 agtgccgcgc accccgagcc cggctcgcgc ttgttgcagg cgtgatagct tggattgcga
     1741 aaatacgtgc aacgcgtgcg ttgcaagaca ttgcccgcca gcgttgccat gttgcgcagc
     1801 tgcgggctcg cggcttgttg caaggcttgc gccagaaccg gatactcgcg gcgcaagacc
     1861 ggatcgtccg cggcttcgcc catccgcacg agtgcgccga gccgcacgcc ttcggcggtc
     1921 ttctcgatcc cgccatggcg cgcttgcaga ggattgatat cgacgatgcg ctccggttgc
     1981 agcacgtcga gcttcatcag atcgaggagc gtcgtgccgc cggcgaggaa ctggctctcg
     2041 gccgtgctcg ccgcgatggc gcgcttcggc gtgtcggcgc taacgtaggc aaagggacgc
     2101 atgggtcacg cctccgtgcg ggacttggcg cgtgcgatcg ccctgacgat gttgggatag
     2161 gcggcgcaac ggcagatatt gccgctcatg aactcgcgaa tttcgtcctc gctgttggcg
     2221 tgcttctcgc gcacgcaggc aatggccgat aggatttgcc cgggtgtgca gtagccgcat
     2281 tggaaggcat cttcttgcat gaaggcctct tgcatgggat gcaaggcacc atcggcggag
     2341 gccaaaccct cgatggtgct gacctccgca cgcaccgccg cggcgagcgt gagacacgcc
     2401 aagacgcgct tgccgccgac gagcacggta caggctccgc attggccgtg atcgcagccc
     2461 ttctttgccc cggtcagggc gagatcatcg cgcaacagat caaggagcgt gcggcgtgga
     2521 tcgatcacca gctcatgtgg gcgcccattg acgctgagcg aaacggaatg tttgtcgccg
     2581 gccacgccgc ctggaccttc gctgccttgc gccgtcgccg cggcggggac caggccacta
     2641 gcggcggcga cagctgtggc ggagagaaac ccgcggcgat tgaccgtgcc gtcggccatg
     2701 ctgaacgctc ctgccgggtt aacccgggat actattggcg tgaactggca gggcctgccc
     2761 tgcgttccgc gcaccgcttc acgctcaggc gagcgaacgc ggccttgagt gcggcggcta
     2821 tcgagccgag cgctatagct caaaggctaa ctaaagagtg cgcccgcggc gccggccgag
     2881 cgcatcctcg gctgcccgag gctcgctcaa gtcctttcag aagctcagcg cctttgcagg
     2941 gcgtaatcgc gcaagctcga ataatccggt agggcctcgg tgcccgtgcg cgacggcgcg
     3001 gggatggcgg gcgaggactg agcctcccca gcaggcggcg cggggatggc gggcgaggac
     3061 tgagcctcgc cagcaggcgg cgcggggacg gtgggcgagg actgcgcctc gccagcaggc
     3121 ggcgcggggc gcaagggcga gggctgcgcc tcgccggccg gcggcgccgg gcgcggcggc
     3181 gcggcgcggc tcagctcctg gcgcgccggc aaatgctcca ccaccgacac gggtgtgccg
     3241 acctcggcca tcgaggccag gtgcacgacc gcggtattga gcatgcggaa gcagccggaa
     3301 gactgcgcct ggccgatgga cttcacgtcg ttggtgccgt gaatgcgata gagcgtatcg
     3361 cccaagtaca aggccatagc gcccaatggg tttttgaggc ccccggtcat cttttccggc
     3421 agc
//
LOCUS       DASTMT010000561         4077 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_983067, whole genome shotgun sequence.
ACCESSION   DASTMT010000561 DASTMT010000000
VERSION     DASTMT010000561.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4077
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_983067"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1578)
                     /locus_tag="VFR00_12850"
     CDS             complement(<1..1578)
                     /locus_tag="VFR00_12850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013418883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HEU0160203.1"
                     /translation="MASRIEDYALIGDCETAALVGRDGSIDWLCWPRFDSGAVFAALL
                     GDDGNGHWLIAPTDAAARRSRKYRGNTLVLETEIVTAEGAVTLIEFMPLRTGGTSHIV
                     RIVQGRRGRVAMHTCLMLRFDYGSIVPWVTRLDDGTIKAIAGPDMAVLSTNVELKAEG
                     FSHRATFSVNAGETATFVLGYASSFQPLPEALCAFAALAETEKAWGTWAAAYRPAGRY
                     AQEALRSLITLKALTYRPTGGIVAAPTTSLPESPGGTRNWDYRYCWLRDATFTLLALM
                     NCGFAGEAEEWRTWLQHAVAGNPAQVQIMYGLAGERRLDEWEIPSLAGYQGARPVRIG
                     NAAAKQLQIDIFGEVMDVLFQSSAGGQAPQVTEWNLQLKLLEHLETIWREPDQGLWEV
                     RGGPRHFTHSKVMAWVALDRAIKSVENFQVEGPFERWCKLRDAIHAEVCERAYDAKLG
                     AFVQSYGASELDASALLIPLVGFLPPNDPRVRSTLEAIRRHLMVDGLVHRYDTHASKD
                     GLPAGEGAFLACSFWFAD"
     gene            1757..2578
                     /locus_tag="VFR00_12855"
     CDS             1757..2578
                     /locus_tag="VFR00_12855"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEU0160204.1"
                     /translation="MPAALALVAFNILEGLRPLAQREPERRLLDRQRRAAAIGLIRDL
                     NPDILVLNEALFCRAHAGQAVDYGSLFGFPHQAAALYDGAWGNAILSRHPITSSGELR
                     IHERGGLTAVIGTPLGALTVASYHPHPSRSPENKARDFANLVAPLPGPLIVCGDFNAI
                     SPEDASDRAQLIAAFRSFASEPEQTVDRFLASGELVFAELAKFGLRDAIPADGRRYSI
                     PTDLINLDKRSGMRIDHILANAAVEVVAGEVVHSPHSNRASDHHPVLLKFRLASP"
     gene            complement(2654..>4077)
                     /locus_tag="VFR00_12860"
     CDS             complement(2654..>4077)
                     /locus_tag="VFR00_12860"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEU0160205.1"
                     /translation="VLLDTEHQRMLAITVNPMSEGGAVLLFEDVTDRQIAEAKIHQLA
                     RFDTLTGLPNRAFLHDQTEAALAALTRRGPFATLFVDLDDFKQVNDTLGHTCGDALLC
                     AVADRLRGIVRGSDVVGRFGGDEFVVLQYPLGELDESAALAEQIVAVLGRPFQINGHQ
                     IVVGASVGIAVAPRDGDSADLLLKNADMALYRVKLNGRGSWQFFEQGMDVKAHARRNL
                     QMDLRNALAADAFQVHYQPLYNLRTKRIATCEALLRWPHPERGMVPPGEFIPIAEEMG
                     LIVEIGRLVLRKACLECARWPNDVRVAVNLSAIQFRRDDMANTIREALQAAKLAPDRL
                     EIEITESAFLDDSEVTRLWLDQLQDLGVRISLDDFGTGYSSLSYLHSFPLNKVKIDRS
                     FLQGLEASSRSLSLLYGVARLSAELDLAVTVEGVETAEQLALIARERSITEVQGFHIG
                     SAVPAEEIRGLLFSPLSQMDRVA"
BASE COUNT          719 a         1368 c         1294 g          696 t
ORIGIN      
        1 gtcggcgaac caaaagctgc aggctaggaa tgcgccctcg ccggcgggta gcccatcttt
       61 gctcgcgtgc gtgtcgtaac ggtgcaccag cccatcgacc atcagatggc gcctgatggc
      121 ctcgagtgtc gagcggaccc gcgggtcatt ggggggcaga aagccgacga ggggaatgag
      181 cagcgcgctg gcatcgagct cgcttgctcc atagctctgc acgaaggcgc cgagttttgc
      241 gtcataggcg cgctcacaga cctcggcgtg gattgcatcg cgcagcttgc accagcgctc
      301 gaacggtcct tcgacctgga agttctcgac gcttttgatg gcgcgatcga gcgccaccca
      361 ggccatgacc ttggaatggg tgaagtgccg tggcccgccg cgcacctccc acaggccctg
      421 gtcgggttcc cgccagatcg tctcgagatg ctcgagcagc ttcaattgca ggttccactc
      481 cgtgacctgc ggcgcctggc caccggcgct gctctgaaac agcacatcca tcacctcgcc
      541 gaagatatcg atctgcaatt gcttggccgc cgcattgcca atgcgcaccg gcctggcgcc
      601 ctggtagccg gcgaggcttg gaatttccca ttcatcaagg cgccgctcgc ccgccaggcc
      661 atacatgatc tgcacctgtg cggggttgcc ggcgacggcg tgctgtagcc acgtgcgcca
      721 ttcctcggcc tcgcccgcaa agccgcagtt catgagggca aggagcgtga aagtagcatc
      781 ccgcagccaa caatagcggt agtcccagtt gcgcgtgccg cctgggcttt ccggcagcga
      841 tgtggtgggc gcggcgacga tcccccccgt cgggcggtag gtcagcgcct tcaaggtaat
      901 gagcgagcgc agcgcctcct gcgcgtacct gccggcaggg cggtaggccg cggcccaggt
      961 tccccaggcc ttctccgtct cggcaagcgc ggcaaaggca cagagcgctt cgggcagggg
     1021 ttggaaggag gacgcatagc cgagcacgaa ggttgccgtt tcgcccgcgt tgacgctgaa
     1081 cgtcgcgcgg tggctgaagc cctctgcctt cagctccacg ttcgtgctga gcaccgccat
     1141 atccggcccg gcgatcgcct tgatggtgcc gtcgtcaagc cgggtcaccc acggcacgat
     1201 cgagccgtag tcgaagcgca gcatcaagca ggtgtgcatg gcaacgcgcc cgcggcgacc
     1261 ttgcacgatg cgaacgatat ggctggtgcc gcccgttctt agcggcatga actcgatcag
     1321 cgtgaccgca ccctcggcgg taacgatctc cgtttccaag accagggtgt tgccacgata
     1381 tttgcgcgac cttcgggccg ctgcgtcggt gggtgcgatc agccaatggc cattgccgtc
     1441 atcccccaac agcgcggcaa acacggcccc cgaatcaaag cgcggccagc atagccagtc
     1501 gatcgagcca tcgcggccaa cgagggcggc ggtctcgcag tcaccgatca gggcgtagtc
     1561 ttcgatacgg cttgccatgg cgccaggcgc tccgcgcaaa cgtcaacaaa atcaacgagg
     1621 cagagggcat cggcgttccc cgccaccggg cgcccgcgcc ttttttcaag cccgccgccg
     1681 ggccgggcac cgggattgct gagcccggcc gcagccgcga attgccagcg cgcacgggtt
     1741 aattcaggat gcaaccatgc cggctgcact cgcgcttgtc gccttcaata tcctcgaggg
     1801 gctgaggccc cttgcgcaac gcgagccgga gcggcgcttg ctcgaccggc aacggcgcgc
     1861 ggcggcgatc gggctgatac gcgatctcaa tccggacatc ctcgtcctga acgaagcctt
     1921 gttttgccgt gcccacgccg gtcaggccgt cgactatggg tcgctgttcg gctttccgca
     1981 ccaggcggcc gcgctttacg atggtgcctg gggcaacgcc attctgagcc ggcatcccat
     2041 cacgagctcc ggggagctgc gcattcacga gcggggcggg ctgaccgccg tgatcggcac
     2101 gcccctcggc gccctgaccg tggcctccta tcacccgcac ccctcgcgca gcccggaaaa
     2161 caaggcacgc gactttgcca acctggtcgc cccgctaccg ggaccgctca tcgtctgtgg
     2221 cgactttaat gcgatcagcc cggaggatgc gagcgaccgg gcccagctga ttgcggcgtt
     2281 ccgcagcttt gcaagcgaac cggaacaaac cgtcgatcgc ttcctcgcca gcggcgaact
     2341 ggtatttgcc gagcttgcca aattcggctt gcgggacgcc attcccgccg atggccggcg
     2401 ctactccatt ccgaccgatc tcatcaacct cgacaagcgc tcgggcatgc gcatcgacca
     2461 catcctcgcc aacgcggcag tggaggtcgt ggctggcgag gtcgtgcata gtccccacag
     2521 caatcgggcc agcgaccatc accccgtgct cctcaagttt cgcctcgcgt cgccttagcc
     2581 ttttgccgac gctggaagcg tccgaggccg ccaaatgcgc acaagccggc acgccggggc
     2641 ctggcgaccg cgctcaggcc accctgtcca tttgcgaaag cggtgagaac agcaaaccgc
     2701 gaatctcctc cgcgggcacg gcgctgccga tgtggaagcc ttgcacttcc gtgatgctgc
     2761 gctcgcgcgc gatcaacgcc agctgctcgg cggtctcgac gccctccacc gtcacggcca
     2821 gatcaagctc ggcactcagc cgcgcaacgc catagagcag gctcagcgag cgactgctcg
     2881 cctcgagccc ttgcaggaag gagcggtcga tcttcacctt gttgagcggg aagctgtgca
     2941 ggtagctcag gctggaatag cccgtgccga aatcgtcgag cgaaatgcga acgcccaaat
     3001 cctgcaattg atcgagccac aggcgcgtga cctcgctatc gtccaagaag gccgactcgg
     3061 tgatctcgat ctcgagacgg tcgggggcga gcttcgcagc ctgcagcgcc tcgcggatgg
     3121 tgttggccat gtcatcgcgc cggaactgaa tggcggagag attgacggcg accctaacgt
     3181 cgttcggcca tcgcgcgcat tccaggcatg ccttgcgcag caccaatctg ccgatctcga
     3241 cgatcaggcc catttcctcg gcgatcggga tgaactcgcc cggcggcacc atcccccgct
     3301 cggggtgcgg ccagcgcagc agcgcctcac aggtggcgat gcgcttggtg cgcaggttgt
     3361 agagcggctg atagtggacc tggaaggcat ccgcggccag cgcgttgcgc agatccattt
     3421 gcagattgcg gcgcgcgtgc gccttcacgt ccatgccctg ttcgaaaaac tgccaagagc
     3481 cccggccgtt cagcttcacc cgatagagcg ccatgtcggc gttcttcaag agcaggtcgg
     3541 cgctgtcgcc gtcacgcggc gccacggcga tgccgacgct ggccccaacg acgatctgat
     3601 ggccattgat ctggaagggg cggccaagca ccgcgacgat ttgctcggcc agggccgcac
     3661 tttcgtcgag ttcccccaag ggatactgca gcaccacgaa ttcatcgccg ccgaagcggc
     3721 cgacgacgtc ggagccgcgc acaatgccgc gcaagcggtc ggccaccgcg caaagcagcg
     3781 catcgccgca ggtgtgcccc agggtgtcat tgacttgctt gaagtcgtcg aggtcgacga
     3841 acagggtggc gaatggaccg cgccgcgtga gcgcggcgag ggcggcctcg gtctggtcgt
     3901 gcagaaaggc gcggttgggc aaacccgtca gggtatcgaa acgggccaat tggtggattt
     3961 tggcctcggc gatttgccga tcggtgacat cctcgaacaa gaggacggcg ccaccttcgc
     4021 tcatcggatt gacggtaatg gcgagcatgc gctggtgctc ggtgtcgagc aaaacct
//
LOCUS       DASTMT010000562         2133 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_1310619, whole genome shotgun sequence.
ACCESSION   DASTMT010000562 DASTMT010000000
VERSION     DASTMT010000562.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2133
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1310619"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            3..>2133
                     /locus_tag="VFR00_12865"
     CDS             3..>2133
                     /locus_tag="VFR00_12865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ligase-associated DNA damage response DEXH box
                     helicase"
                     /protein_id="HEU0160206.1"
                     /translation="MLIAPTGAGKTLAGFLPSLVALMPRPRPASGRSIHTLYVSPLKA
                     LTSDIARNLMVPLTEMALPVTVETRTGDTPVARKRRQRRRPPDLLLTTPEQIALLLSH
                     AEASGIFAGLETVIIDEIHALTPNKRGDLLALDLARLASIAPKQVRIGLSATVARPSE
                     LRAYLLPQTEARVTRLADLLTASGGIAHDITMLETDAALPWAGHTTRYATADILQAIK
                     AHRLSLLFVNTRNQAELLFQELWRLNEDNLPIALHHGSLDAARRRKVEAAMAAGSLRA
                     VVSTSTLDLGVDWADVDLVINVGAPKGASRLLQRVGRANHRLEEASQALLVPSNRFEV
                     LECRAAIEAAEAGAHDAATHRTGALDVLAQHVWGMACSGPIKPGALYREVRSAQPYAN
                     LTRPSFDRVLDFVATGGYALRAYERYARLRQAGAGRLRLAHPRLAQHYRMNVGTIVEA
                     PMIKIRLVGRRGKRRAGGSALAGGRLLGEVEEYFVEQLKFGDTFAFAGEVLRFEGLQN
                     TDAFVTRTSDPDPMIPSYEGGKFPLSTHLADRVRSLLAEPSRWRQLPQAVREWLLRQQ
                     DVSSLPAPGEVLIESFARARRHYLIAYPFEGRLAHQTLGMLLTRRLDRIGAKPLGFVA
                     NDYALAIWSLGDLAHLQRQGRLDLAALFAEDMLGDDLEAWLAESSLLRRTFRINAVIA
                     GLIERRHAGRIKSGRQITISSDLIYDVL"
BASE COUNT          356 a          726 c          676 g          375 t
ORIGIN      
        1 cgctgctcat cgccccgacc ggtgccggca agaccctcgc cggttttctg ccaagcctgg
       61 tggcgctaat gccccgcccg cgcccggcaa gtggccgctc catccatacg ctctacgtct
      121 cccctctcaa ggcgctgacc agcgatattg cccgcaatct gatggtgccg ctgacggaaa
      181 tggccctgcc cgtgaccgtc gagacccgta cgggcgacac gccggtggca cgcaagcggc
      241 ggcaacgtcg gcgcccgccc gatctcctcc tgacgacgcc tgagcagatc gcgctgctgt
      301 tgtcgcatgc cgaagccagc ggcatcttcg ccggcctcga gaccgtgatc atcgatgaga
      361 tccatgccct gacgcccaac aagcgcggcg atctgctggc gctcgatctc gcccgcctcg
      421 ccagcatcgc gccaaagcaa gtgcgcattg gcttgtcggc gacggtcgcc cgccccagcg
      481 agctgcgcgc ctatttgctg ccgcagaccg aggcccgcgt gacacgcctg gcagacctgc
      541 tcacggcctc gggcgggata gcacacgaca tcaccatgtt ggaaaccgat gcggcactgc
      601 cttgggccgg ccacaccacc cgttacgcca cggccgacat tctgcaagcc atcaaggcac
      661 atcgcctgtc gctgttgttt gtcaacacgc gcaatcaagc ggagctgctg ttccaggagc
      721 tttggcgcct caacgaggac aatctgccga tcgcattaca tcacggctcg ctagatgcgg
      781 cgcggcggcg caaggtcgaa gcggcaatgg cggcgggttc attgcgggcg gtggtgtcga
      841 cttcaaccct cgatctgggc gtcgactggg ccgacgtcga cctcgtcatc aatgtcggtg
      901 cgccgaaggg agcgagccgg ctgctgcagc gcgtcggccg ggccaatcac cgcctcgaag
      961 aggcatcgca ggccttgttg gtaccgtcaa atcgtttcga ggtgcttgaa tgccgcgccg
     1021 ccatcgaggc cgccgaagcg ggggcacatg atgccgcgac acatcgcacg ggcgcgctgg
     1081 acgtgctggc gcagcacgta tggggcatgg cttgcagcgg gccgataaaa ccgggcgccc
     1141 tttatcgcga ggtgcgcagc gcccagccct atgcaaacct gacgcggccc agcttcgatc
     1201 gtgttctcga tttcgtcgcc accggcggct atgcgctacg cgcctacgag cgctacgccc
     1261 gattgcggca ggccggcgcc ggtcgcctgc gcctcgccca cccacgcctg gcgcaacact
     1321 accgcatgaa tgttggcacc atcgtcgagg cgccgatgat caagatccgg ctggtcgggc
     1381 ggcgcggcaa acgccgagcc ggcggcagcg cattggcggg cggacggctc ctgggcgagg
     1441 ttgaggaata tttcgtcgaa cagttgaagt tcggcgacac cttcgccttt gccggtgagg
     1501 tgctgcgctt cgaaggcctg cagaacaccg atgccttcgt cacacgcacg agtgatcccg
     1561 atccaatgat cccgtcctat gagggaggca aattcccgct ttcgacgcat ttggccgacc
     1621 gggtccgcag cctgctcgcg gagccaagcc gctggcgcca actaccgcag gcggtgcgcg
     1681 aatggctgtt gcgtcagcag gacgtttcca gcctgccggc gcctggcgag gtcctgatcg
     1741 agagttttgc gcgcgcgcgt cggcactatc tcatcgccta tccctttgag ggccggctgg
     1801 cccatcagac gctcggcatg ctgctgacgc ggcggctgga tcgcataggg gccaagccgt
     1861 taggctttgt cgccaacgac tatgcgctgg cgatctggtc attgggcgac ctggcgcacc
     1921 tgcaacggca aggacggctc gatctagcag ccctgttcgc cgaggacatg ctgggcgacg
     1981 acctcgaggc ttggctggcc gaatcgagcc tgctgcggcg gacttttcgc atcaacgcgg
     2041 tcattgccgg cctgatcgag cgccgtcatg ccggcaggat caagagcggc cgtcagatca
     2101 ccatttcctc cgatctcatc tacgatgtgc tgc
//
LOCUS       DASTMT010000563         6703 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_1965291, whole genome shotgun sequence.
ACCESSION   DASTMT010000563 DASTMT010000000
VERSION     DASTMT010000563.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 6703)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6703)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6703
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1965291"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..345
                     /locus_tag="VFR00_12870"
     CDS             <1..345
                     /locus_tag="VFR00_12870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0160207.1"
                     /translation="IAAIFRFVQLIWNWSVEQIMRVLDAPWQNWPFWKQVLLVLIAAG
                     VVWALYKAAAELWEAGERTLAAFAGLLGALVRTLPSVLIAGVIAMSGIWVINNLDLSR
                     IALPASWQLGAR"
     gene            complement(353..1843)
                     /locus_tag="VFR00_12875"
     CDS             complement(353..1843)
                     /locus_tag="VFR00_12875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0160208.1"
                     /translation="MQGKVDAETEAGTGTPTPGGSASGVVETDIPARLDRLPWGRFQT
                     LVVLALGITWILDGLEVTLVGAVAGALKQSPVLQLSNTEVGLASSAYLAGAVLGALFF
                     GWLTDRLGRKKLFFITLAVYLVATAATALSWSFWSFALFRFLTGAGIGGEYTAINSTI
                     QELVPARVRGWTDLAINGSFWLGAAMGAAGSIVLLDPALLPADLGWRLAFAIGATLSL
                     VILFMRLWLPESPRWLATHGRLPEAEAIVAGIEENFRNQGHALTEGPFAKIRLKARTH
                     TPIREVVSTLVGSQRRRTAVGLTLMAAQAFFYNAIFFTYALVLTDFYGVPADQVGWYI
                     LPFAAGNFLGPALLGRLFDTLGRRPMITFTYAASGLLLAFTGYLFAADLVSARGLTLG
                     WSVIFFFASAAASSAYLTVSETFPLEIRALAIAIFYAIGTGIGGVIGPWLMGFLIETG
                     SRPSVFAGYLLGAGLMLLAALVMARWGVAAERKPLEAVARPLASVD"
     gene            complement(1827..2303)
                     /locus_tag="VFR00_12880"
     CDS             complement(1827..2303)
                     /locus_tag="VFR00_12880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0160209.1"
                     /translation="MVDITHTPDAYRPRTNQATARALSIFAALAVDAGLVWLLYVRAK
                     FNPPFGDAEMFWVRIVAGFGAYAVLQQWTLANQTGRGDDIVASMDKAVALSPLLLAAA
                     LEAYWVGMGTWVELSWRHHAAALLWSAFALTDYFATDTTNQRLSAQRFHGGDAGES"
     gene            2473..2661
                     /locus_tag="VFR00_12885"
     CDS             2473..2661
                     /locus_tag="VFR00_12885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0160210.1"
                     /translation="MSRTPPRITIDQQIADAQRRIREQKLRLQRMITQGTIAQSDDDL
                     LREMYVTLRELQAARVRG"
     gene            2825..2998
                     /locus_tag="VFR00_12890"
     CDS             2825..2998
                     /locus_tag="VFR00_12890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HEU0160211.1"
                     /translation="MGNLLHYALVFLVVALIAAAVGFGGVAGFAMEAARLLFWVFIVL
                     FVISLIAGLVRRA"
     gene            3155..4321
                     /locus_tag="VFR00_12895"
     CDS             3155..4321
                     /locus_tag="VFR00_12895"
                     /inference="COORDINATES: protein motif:HMM:NF014036.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase domain-containing protein"
                     /protein_id="HEU0160212.1"
                     /translation="MTTSADPQQPIAERARSFAPVVWLLGKVQSGKTSIIRELTQASD
                     AEIGSGFRACTKTARVFDFPAQAPIIRFLDTRGLGEVDYDPAADISFCEGRAHLILAV
                     MKALDLEQSVVLDIVRAVRRRHPEWPVVVAQTTVHEAYALGVGHVLPYPFAAPGTPGV
                     PENLLRALAYQRSLFEGLMGRAQPHFVPIDFTHVEDGLMPNAYGREALIDTLIAAAPA
                     AVALALSELPGSSAGRAAEQYNAHILGFALAAGASDAVPVAGVVAVPMVQAAMLRQLS
                     KLHGARWDRRAYAEFAAALGAGTLVRTASSFGVRQLVKLIPVYGQTAGAAAAAAASFA
                     ATYAMGKAANYFLVRRQQGLRADEVSAVYRRALSEAFELAKARAIGPGAREAKP"
     gene            4318..5925
                     /locus_tag="VFR00_12900"
     CDS             4318..5925
                     /locus_tag="VFR00_12900"
                     /inference="COORDINATES: protein motif:HMM:NF014036.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase domain-containing protein"
                     /protein_id="HEU0160213.1"
                     /translation="MTSREPRSPYPRWAMLIVALLLPAASLMPFGSLWLWQHGYIVYW
                     AIVICVLVLAVYYLEQRLIVPSAAPAALEAEAPDPGDANWTPLQAQAWDDVLRLAAEV
                     NPARLTSRDALWALGLEAIEVVAKRLHPERGDPLLQFTLPEALTVLERASVNLRRFVL
                     TSFPLGDRITVAQFMWLYRWRGALDLVEKGYDLWRVVRLLNPVAAVTQELRERFTRQI
                     YEMGREHLARRIARAFVKEVGRAAIDLYGGNLRVAPERVTTHVTTASRADLQAAEARA
                     LEPVRILVAGQVGVGKSSLVNALANAVEAAVDTLPSTAQFTAYRLSREGLPAALIIDS
                     PGLAEANGLPGLIEAAHSCDMILWVTSATRAARQVDAAAVRAIREHFSAEPNRRRPPM
                     LLILTHIDRLRPFNEWEPPYDLATPTRAKARSIREALEAAGTELGFAANEIVPVRIDI
                     AVAPYNVDALWVKIIEFMSEAQRARLLRTLRDIESASNWRALWSQAVNAGRVIGETFF
                     TRSTDDVADDEAGRRQGSRSTGKTQEG"
     gene            5855..>6703
                     /locus_tag="VFR00_12905"
     CDS             5855..>6703
                     /locus_tag="VFR00_12905"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEU0160214.1"
                     /translation="MMSQTMKPAAGKEVAQPAKPKRVEGEAGARNEPLAPAAAEVDSW
                     ARASQIAVVGLFIIAVLWCAYVAQPVLVPVLLAWAIATIVLPIVKWLGERGIPRVPAA
                     VLVTLFLFLLIISLLTLLSTPVAYWLGRATEIGALIKQKLQSMGQPLVLLDELQKALN
                     AIGSGESTLRVEAQQSTLVGTIFSILTPAVSQFVLFLGALVFYLIYQKRLRSTVVYLL
                     RDRQARLATLRTLNDIDENMTTYFGTFTIVNICLGLVTVALTWFVGLPNPLLWGVLAG
                     LLNYIPY"
BASE COUNT         1146 a         2131 c         2245 g         1181 t
ORIGIN      
        1 atcgcggcga tctttcgctt tgtgcaactg atctggaatt ggtccgtcga gcaaatcatg
       61 cgggtgctcg atgctccctg gcaaaattgg ccattttgga agcaggtctt gctggtgttg
      121 atcgcggcgg gcgtggtttg ggcgctttat aaggcggccg ccgaattgtg ggaggcgggc
      181 gagaggacgc tggcggcctt tgccggctta ctgggagcgc tggtgcgcac ccttccaagc
      241 gtgctcattg ccggcgtcat cgccatgagc ggcatctggg tgatcaacaa cttggaccta
      301 tcgcgcatcg ccttgccggc ctcctggcag ctgggcgcgc gctgaggcca gctcagtcga
      361 cggacgcgag cgggcgtgcc acggcctcca acggcttgcg ttcggcggcc acgccccagc
      421 gcgccatcac cagggcggcc agcagcatca ggccggcgcc cagcaggtag ccggcgaaca
      481 cgctcgggcg ggagcccgtc tcgatcagga agcccatcag ccaggggccg atgacaccgc
      541 cgatgccggt gccgatcgca tagaaaatcg caatcgccaa ggcgcggatc tccaagggaa
      601 aggtttcgct gaccgtgaga taggccgagc tcgccgcggc cgaggcaaaa aagaaaatca
      661 ccgaccagcc cagcgttagc ccgcgcgcgg agaccaggtc ggccgcaaac aggtagccgg
      721 tgaacgccaa gagcaggccc gaggcggcgt aggtgaacgt gatcatcgga cgtcgcccga
      781 gcgtgtcgaa caatcgcccg aggagggctg ggcctagaaa gttgccggcc gcaaacggca
      841 aaatgtacca acccacctgg tcggccggaa cgccatagaa gtcggtcagc accaacgcgt
      901 aagtaaaaaa gatggcgttg tagaagaagg cttgcgccgc catcagcgtc agtccgacgg
      961 ccgtgcgccg ccgctgcgag ccaaccaggg tcgataccac ctcgcgaatc ggcgtgtggg
     1021 tgcgggcctt gaggcggatt ttcgcaaacg gcccctcggt cagggcgtgg ccctggttgc
     1081 gaaaattttc ttcgatgccg gccacgatcg cctcggcctc gggcagccgg ccatgcgtgg
     1141 cgagccagcg cgggctttcc ggcagccaca ggcgcataaa gagaatgacg aggctcaagg
     1201 tggcgccgat ggcaaaagcc aggcgccagc ccagatcggc cggcaacagc gcgggatcga
     1261 gaaggacgat ggagccggcg gcgcccatgg cggcgccgag ccaaaagctg ccgttgatgg
     1321 cgagatcggt ccagcctcgc acacgtgccg gcacgagctc ctgaatggtg gaattaatgg
     1381 cggtgtattc cccgccgatt ccggccccgg tcaggaaacg aaaaagcgca aagctccaaa
     1441 aactccagga cagggcggtg gcggccgtcg ccacgagata gacggcaagc gtaataaaga
     1501 agagcttttt gcgccccagg cggtcggtca gccagccgaa gaacagggcg ccgagcacgg
     1561 cacccgcgag ataagcgctg cttgccaagc cgacctcggt gttgctgagc tggagcacgg
     1621 ggctctgctt cagggccccg gccaccgccc cgaccagggt gacctcaagg ccgtcgagga
     1681 tccaggtgat gcctagggcg agaacgacca gcgtctgaaa gcgtccccag ggcaagcggt
     1741 caagacgcgc cgggatatcc gtctccacga cgccgctcgc cgacccgccc ggcgtaggcg
     1801 tgcccgtgcc ggcctcggtt tctgcgtcaa ctttcccctg catcgccgcc gtggaatcgc
     1861 tgcgcgctca gtcgctggtt ggtggtgtcg gtggcaaagt aatccgtcaa ggcaaaggcg
     1921 ctccacaata gcgcggccgc atgatggcgc caggacagct ccacccaggt gcccatcccc
     1981 acccaatagg cttccagcgc ggcggctagc aacagcgggc tcaaggcgac cgccttgtcc
     2041 attgaggcga cgatatcatc gccgcgtccg gtttggttgg cgagggtcca ttgctgcagc
     2101 acggcgtagg cgccgaagcc ggcgacaatg cggacccaaa acatttcggc atcgccgaag
     2161 ggaggattga acttggcgcg cacgtagaga agccaaacca acccggcgtc gacggcgagc
     2221 gcggcgaaga tgctcaaggc ccgggcggta gcctggttgg tgcgggggcg ataggcatca
     2281 ggagtgtgcg tgatatccac catcgaaccc tccccctatg cgccgaagcc cgagccctac
     2341 ccttggccta aacgcagagc cgcgcgggag gttgctgata agcgcgcagg tcgctgcccg
     2401 gggccgggaa caaagcaggc cccggcgtcg ttgtcgagtg ggggcgcgcg cgccggccgc
     2461 aggagatttc cgatgtctcg aaccccgccc cgcatcacca ttgaccagca gatcgccgac
     2521 gcgcagcggc gcatccgcga gcaaaagctc cgcctgcagc gcatgatcac ccagggtacc
     2581 atcgcccaat ctgatgatga tctcctgcgt gagatgtacg tcacattgcg cgagctgcag
     2641 gcggcccgcg tgcgtggtta atgcgacgac cgatcggcag ccgttgtctt ggcggtctgg
     2701 caagccagcg ttcgggcaac gctggggccg cgtggtggac aggaaccgtg gcgggcgcgc
     2761 cgcgttttta aggcgacgat caagtttgcc gctctaagct gcggcctcaa ctggagattt
     2821 gagcatgggc aatctacttc actacgcgct ggtgttctta gtggtggcgc tcatcgccgc
     2881 cgccgtgggc ttcggtggtg tcgccggctt tgcgatggag gccgcccggc tgctgttctg
     2941 ggtgttcatc gtgctattcg tgatctccct gattgccggg ctggtacggc gcgcctgagg
     3001 cgtgccgcgc gaaccctgaa aagcaatgag aatgatgcaa tggtaagggg cggcgttgtc
     3061 aaaggacgcc gcccttgctt tgctgcccgt tgctggtcga gcctggcccg gccggccaac
     3121 cgtcatcgat gcttggccag gtttgcgcac cgccatgacc acctccgccg atccccagca
     3181 gccaatcgca gagcgggcgc ggagcttcgc ccccgtggtc tggttgcttg gcaaggtgca
     3241 gagcggcaaa acctccatca tccgcgagct gacccaggcc agcgatgcag agatcggcag
     3301 cggctttcgt gcctgtacca agacggcgcg cgtgttcgat tttccggcac aagcccccat
     3361 cattcgtttt ctcgatacgc gtggcctggg cgaagtggac tacgaccccg cggctgatat
     3421 cagcttttgc gagggccgcg cccacctcat tctggccgtc atgaaggcgc tcgatctgga
     3481 acagagcgtc gtcctcgaca ttgtgcgcgc ggtgcggcga cggcatccgg aatggccggt
     3541 cgtggtggcg caaaccaccg tgcacgaggc ctatgcgcta ggggtcggtc acgtcctgcc
     3601 ctatccgttc gcggcgcccg ggacaccggg ggtgccggag aacctcttgc gtgccttggc
     3661 ctatcagcgc tcgctgttcg agggcctgat gggtcgcgcc cagccgcact ttgtgccgat
     3721 cgattttacc cacgtcgagg atgggctcat gcccaatgcc tacgggcgcg aggctctgat
     3781 cgataccctg atcgccgcag cgcccgcagc cgtcgcgctg gctttgtcgg aactgcccgg
     3841 ctcaagcgcg ggccgcgccg ccgagcagta caatgcccac atccttggct ttgcgctggc
     3901 ggcgggggcg agcgatgcgg tgccggtcgc cggcgtcgtc gcggtgccga tggtgcaggc
     3961 cgcaatgctt cggcaactct ccaagctgca cggcgcccgc tgggacaggc gggcctatgc
     4021 ggaattcgcc gccgcgctcg gcgccggaac gctggtgcgc acggcctcca gcttcggcgt
     4081 gcgccagttg gtcaagctca ttccggtata tggccagacc gccggcgccg cggcggcggc
     4141 ggcggcgagc ttcgcggcca cttacgcgat gggcaaggct gccaactact tcctggtccg
     4201 acggcagcag ggtctgcgcg ccgatgaagt gagcgccgtc tatcggcgcg cgctgagtga
     4261 agcgttcgaa ctggccaagg cgagggccat tggtccgggt gcgagggaag ccaaaccatg
     4321 acgtcgcgcg agccgcgctc accctatccg cgctgggcca tgttgatcgt cgccttgctg
     4381 ctacccgcgg cgagcttgat gccttttggg agcctctggc tctggcagca cggctacatc
     4441 gtctactggg caatcgtgat ctgcgtgctt gttctcgccg tctattacct ggagcaacgc
     4501 ttgattgttc ccagcgccgc gccggcggcg ctcgaagcgg aggcgcccga tccgggcgac
     4561 gccaattgga caccattgca ggcgcaggcg tgggacgatg tgctgcggct cgccgccgag
     4621 gtgaaccccg cgcgcctgac gagccgcgat gcgctgtggg cgctcggtct cgaagccatc
     4681 gaggtggtgg cgaagcgcct gcatccggag cgtggggatc cgctgttgca gttcacgctg
     4741 ccggaggcgc tgaccgtgct cgaacgtgcc agcgtcaacc tgcgtcggtt tgtactgacg
     4801 tcctttccgc tgggagaccg catcaccgtt gcgcaattca tgtggcttta tcggtggcga
     4861 ggcgccctgg atctcgtcga gaaaggctat gatttgtggc gcgtcgtgcg gcttctcaat
     4921 cccgtggccg cggttaccca agaattgcgc gagcgcttca cgcgacagat ttacgaaatg
     4981 gggcgcgagc acctggcgcg gcggatcgcg cgcgcttttg tcaaggaagt aggacgcgct
     5041 gccatcgatc tctacggagg caacctgcgc gttgcccccg agcgcgtaac cacgcatgtc
     5101 acgaccgcct caagggcgga cttgcaggcc gcggaggcgc gcgcgctgga accggtgcgc
     5161 atcctggtgg cgggtcaggt cggcgtcggt aaatcgagcc tggtcaatgc gctcgccaat
     5221 gccgtggaag cggcggtcga cacgctgccc tccacggcgc agttcaccgc ctatcggctg
     5281 agccgcgagg gactgcctgc ggccctcatt atcgacagtc cggggcttgc cgaggccaac
     5341 gggctccccg gcctgatcga ggcggcgcac agctgcgaca tgatcttgtg ggtgacctcg
     5401 gccacccgtg cggcgcgtca ggtcgatgcg gcggcggtgc gcgccattcg cgaacacttc
     5461 agcgccgagc ccaaccgccg ccggccaccc atgttgctga ttctcacgca catcgacagg
     5521 cttcgcccgt tcaacgagtg ggagccgccc tatgatcttg ccacgccgac gcgtgccaaa
     5581 gcccgatcga tccgggaagc gctcgaggct gccggcacgg agctcggctt tgccgccaac
     5641 gagatcgtgc ccgtgcgcat cgacatcgcc gtggcgccct acaacgtcga tgcgctgtgg
     5701 gtgaagatca tcgagttcat gtccgaggcc cagcgcgcgc gcctgctgcg caccttgcgc
     5761 gacatcgaga gcgcgtcgaa ttggcgtgcg ctgtggtcgc aggcggtcaa cgcggggcgc
     5821 gtcattgggg agaccttttt cacgcggagt acagatgatg tcgcagacga tgaagccggc
     5881 cgccggcaag gaagtcgctc aaccggcaaa acccaagagg gttgagggcg aagccggcgc
     5941 gcgtaacgag ccactcgcgc ccgcggctgc cgaagtcgat agctgggcgc gggcgagcca
     6001 gattgccgta gtcggcctct tcatcattgc cgttttgtgg tgcgcctatg tggcccagcc
     6061 ggtgctcgtg ccggtgttgc tcgcctgggc catcgccacg atcgtgttgc cgatcgtgaa
     6121 atggctgggc gagcgcggca tcccgagggt gccggccgcc gtccttgtca cgctcttctt
     6181 gttcctgctg atcatcagcc tgttgacgtt gctctccacg ccggtcgcct actggctggg
     6241 ccgggccacg gagattggcg cgctgatcaa gcagaagttg caaagcatgg gacaaccgct
     6301 cgtgcttctc gacgagctgc agaaggcgct caacgccatc ggttcggggg agagtacgct
     6361 gcgggtcgag gcgcagcaat cgacgctggt cggcacgatc ttttccatcc tcacaccggc
     6421 agtcagtcaa ttcgtgctgt tcctgggtgc gctcgtcttc tacctcatct atcagaagcg
     6481 gctgcgcagc acggtcgtct atctgctgag agatcggcag gcccggttgg cgacgctgcg
     6541 gacgctgaat gacatcgatg agaatatgac gacctacttt ggcactttca ccatcgtcaa
     6601 tatttgtctg ggcctcgtca cggtggccct cacttggttc gttggtttgc ccaatccgct
     6661 gctgtggggg gtcttggccg gccttcttaa ctatattccc tac
//
LOCUS       DASTMT010000564         3879 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_4263369, whole genome shotgun sequence.
ACCESSION   DASTMT010000564 DASTMT010000000
VERSION     DASTMT010000564.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3879)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3879)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3879
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4263369"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..276)
                     /locus_tag="VFR00_12910"
     CDS             complement(<1..276)
                     /locus_tag="VFR00_12910"
                     /inference="COORDINATES: protein motif:HMM:NF014663.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urea amidolyase"
                     /protein_id="HEU0160215.1"
                     /translation="MSPALRLLAAGLLTTLQDKGRVGYQQLGIPVSGALDPVSLAAAN
                     LLVGNPPDCAALEIAYHGPTLVVEADSVRIACAGAAAPIELSGAAAPA"
     gene            complement(273..1019)
                     /gene="pxpB"
                     /locus_tag="VFR00_12915"
     CDS             complement(273..1019)
                     /gene="pxpB"
                     /locus_tag="VFR00_12915"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020187403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HEU0160216.1"
                     /translation="MTAAEHLLPRAKFLAAGDTALVVEFGDRIDPQVSARVLALARRV
                     EAAAIAGVVETVPTFRSLMVHYDPLSISNAELRARLEPLLADLAGQVPAGRHWRLPAC
                     YDASLGLDLAEVAERTGLSAAAVVEAHSTAPFRVYMVGFLPGFAYLGGLPSALELPRR
                     ENPRLKVDAGSIAIAMAMSVIYSIESPGGWNILARTPIRLWDLRRDPAALMNAGDSVS
                     FEPISRAEYEELAGKAQAGELRVTPQPRDA"
     gene            1134..1907
                     /locus_tag="VFR00_12920"
     CDS             1134..1907
                     /locus_tag="VFR00_12920"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006019473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HEU0160217.1"
                     /translation="MARTVNLNADIAEGWGAYDIGNDAELMKVIKSASVACGFHAGDP
                     NTMHRLCLLAKQEGVSVGVHPGFNDLWGFGRRRIQMSPRDLELMVAYQIGALQAMAAY
                     AGLKVTHLKAHGALNNMAAEDEGYAEAIGRAIKAVDRDIIYVVLFGSAMEKAARKLGL
                     RLALEGFPDRRYQEDGNLASRAIPGTVLKDPKQAGEQALRMVRDGEIVALSGKRIKVK
                     VDTLCVHGDEASGVVVARGIRAALEEAGIAVVPLPDMQL"
     gene            1925..2617
                     /gene="npdG"
                     /locus_tag="VFR00_12925"
     CDS             1925..2617
                     /gene="npdG"
                     /locus_tag="VFR00_12925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006019474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent F420 reductase"
                     /protein_id="HEU0160218.1"
                     /translation="MTEPPLQPAVGAKPHLAIIGGTGPLGSGLARRLLLAGYGVVIGS
                     RTAAKAEAATAALAAEIGPGRLHGADNLVAARQGDIVFITVPWASQAAILEEIKPALA
                     GKLVVDTTVPLVPPKVARVQLPKEHSAALAAQVRLGPEVRVVSAFHNVAAQKLAAGGP
                     IDCDVLVFGDDPKDRQIVVELATAMGLEAVHAGPLANSVAAEALTSVLIGINRNYKAE
                     GAGIRITGITRP"
     gene            2651..3433
                     /gene="cofE"
                     /locus_tag="VFR00_12930"
     CDS             2651..3433
                     /gene="cofE"
                     /locus_tag="VFR00_12930"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006019475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043773 - coenzyme F420-0 gamma-glutamyl
                     ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HEU0160219.1"
                     /translation="MGLLPGTLELSVTALTGLPEVAPGADLAGLLIAALNEGGLLPRR
                     QDILVVTQKVVSKAEGRYLDLDRLAPSRRAEELARITGKDARLIEAVLSQADEVLRAK
                     RNVLIVATGQGLVIANAGIDQSNLTPEDHGQRVLLLPEDADLSAGRLKARLDQHFQCD
                     LGLIISDSAGRAWRLGTVGIAIGAAGVPALLDRRGEPDRAGRPLEVTEVGFADAVAAM
                     AVLAMGEAAEARPAALVRGLSWSAPARPAAALVRPKPDDMFR"
     gene            3442..>3879
                     /locus_tag="VFR00_12935"
     CDS             3442..>3879
                     /locus_tag="VFR00_12935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018389508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043743 - LPPG:FO 2-phospho-L-lactate
                     transferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate transferase CofD family
                     protein"
                     /protein_id="HEU0160220.1"
                     /translation="MSRFVALSGGVGGAKLALGLAHLLGERLSLIVNTGDDFEHLGLY
                     VSPDLDTALYTLAERVNPATGWGRSAETWTFMAALEELGGPTWFNLGDGDLATHVLRS
                     ERLRAGATLTAVTTELARRLGVKIEILPMCDSELRTVLETDAGT"
BASE COUNT          630 a         1263 c         1356 g          630 t
ORIGIN      
        1 ggctggcgcg gcagccccac tgagctcgat tggcgccgcg gcgccggcgc aggcgatgcg
       61 gacgctatcg gcctccacca ccagcgttgg gccgtgatag gcgatttcga gggccgcgca
      121 gtcgggtgga ttgcccacca ggaggttggc ggcggcgagg cttaccgggt cgagtgcccc
      181 tgaaaccgga atgccaagct gctggtagcc gacccggccc ttgtcttgca gcgtcgtgag
      241 caagcccgca gcaagcaggc gcagggcagg gctcatgcgt cgcgcggctg cggcgtcacg
      301 cgcaactccc cggcctgcgc tttcccggca agctcctcgt actcggcgcg cgagatgggc
      361 tcgaagctca cgctatcgcc cgcgttcata agggccgccg ggtcacggcg caaatcccac
      421 agtcggatgg gcgtgcgcgc gaggatgttc cagccgccgg ggctttcgat gctgtagatc
      481 acgctcatgg ccatggcgat ggcaatcgat ccggcatcga ccttgagccg cgggttctcg
      541 cgccgcggca gctccagcgc gctgggcagg ccaccgagat aggcaaagcc gggcaggaag
      601 cccaccatat agacgcgaaa gggcgccgtg ctgtgcgcct caaccacggc ggcggcgctg
      661 aggcccgtgc gctcggcgac ctccgccaaa tcgaggccaa ggcttgcatc atagcaggcg
      721 ggcaggcgcc aatgccgtcc ggctggcact tggccggcca ggtccgcgag taaaggttca
      781 aggcgcgctc gcaactcggc attgctgatg ctgagaggat cgtagtgcac catgagcgag
      841 cggaaggtcg gcaccgtctc gaccacgccg gcaatggcgg cagcctcgac gcggcgcgcc
      901 agcgccagca cgcgggcgct gacctgcggg tcgatacgat ccccgaactc taccaccagc
      961 gcggtatcgc cggcggccag aaatttcgcg cgtggcagga ggtgttcggc ggcggtcaat
     1021 gcggcacctg agcccgtcgt cggcttcgtt cacattgtct ggttcggcta tagatagtac
     1081 gatggggcga tcggcggcta ccgccatttt tgcaggtgaa ggcgaggcat agcatggcga
     1141 ggacggtcaa tctcaatgcg gacatcgccg agggatgggg tgcctacgac atcggcaacg
     1201 acgccgagct gatgaaggtc atcaagtcag ccagcgtggc ctgcggtttc catgcgggtg
     1261 atcccaacac catgcaccgc ctgtgcctgc tggcgaaaca ggaaggggtg agcgtcggcg
     1321 tgcatccggg gttcaacgac ctttggggct tcggccggcg ccgcatccag atgagccctc
     1381 gcgatctgga attgatggtc gcctatcaga tcggtgcgct gcaggcgatg gcggcctacg
     1441 ccggcctcaa ggtgacgcat ttgaaggccc acggtgccct caacaacatg gccgccgagg
     1501 acgagggcta cgccgaagcc atcggccgtg cgatcaaggc cgtcgatcgc gacatcatct
     1561 acgtcgtgct cttcggttcc gccatggaga aggccgcgcg caagctcggt ttgcgcctgg
     1621 cgctggaagg cttccccgat cggcgctacc aggaggacgg caacctcgcc tcccgcgcga
     1681 tccccgggac cgtgctcaaa gatcccaagc aggcgggcga gcaagccttg cgcatggtgc
     1741 gcgatggcga gatcgtggcg ctttccggca agcgcattaa ggtaaaggtc gacaccttgt
     1801 gcgtgcacgg tgacgaggcg agcggtgtgg tggtggcgcg cggcatccgc gcggccctcg
     1861 aggaggctgg tatcgccgtg gttcctcttc ccgacatgca gctttgaaaa ccggatcgct
     1921 gccggtgacc gagccccctc tccagccagc ggtcggcgcc aagccgcacc ttgccatcat
     1981 tggcggcact ggccccttgg gttccggctt ggcccggcgg ctgctgctgg ccggctatgg
     2041 cgtggtgatc ggatcacgca cggccgccaa ggccgaagca gcgacggcgg cgcttgccgc
     2101 ggagatcggc cccggtcgct tgcacggggc cgacaacctg gtggcggcga gacagggcga
     2161 tatcgtcttc ataaccgtgc cgtgggcgag ccaggccgcc atcctggagg agatcaagcc
     2221 cgcgctcgcc ggtaagctcg tggtcgatac caccgtgccc ttggtgccac ccaaggtggc
     2281 gcgcgtgcag ctgcccaagg agcattcggc ggccttggcc gcgcaagtcc gactcgggcc
     2341 ggaggtgcgc gtggtctcgg cctttcacaa cgtggcggcg caaaaattgg ctgccggtgg
     2401 gcccatcgat tgcgacgtcc ttgtctttgg cgatgatccc aaggatcgcc agattgtggt
     2461 cgagctcgcc acggcgatgg ggctcgaggc cgtgcacgcg ggcccgctcg ccaactcggt
     2521 ggcagccgag gcgctgacct cggtgctgat cggcatcaac cgtaactaca aggccgaggg
     2581 tgcgggcatc cgcatcaccg gcattacacg accctagcgc acaggcgaaa ccgcacaaca
     2641 ggcgccgcga atgggccttc tacccggcac gctcgagctc agcgtcaccg ccttgacggg
     2701 cctgcccgag gtggcgccgg gcgccgattt ggccggcttg ttgattgccg cgctcaacga
     2761 aggagggctt ttgccccggc ggcaggatat cctggttgtg acgcagaagg tcgtgtccaa
     2821 ggccgaagga cgctacctcg atttggatcg gctcgcaccc tcgcggcgcg ccgaggagct
     2881 ggcgcgcatc accggcaagg acgcgcgtct gatcgaggcg gtcttaagcc aagccgacga
     2941 ggtcttgcgg gccaagcgca acgtgctgat cgtcgccacc gggcaagggc tcgtcatcgc
     3001 caacgccggc atcgatcagt ccaacctgac gccggaggat cacgggcagc gtgttttgct
     3061 gctgcccgaa gatgccgatc tgagcgcggg gcggctgaaa gcgcgcctgg atcaacactt
     3121 ccagtgcgac ttgggcctga tcatttccga tagcgctggc cgggcctggc ggctcggcac
     3181 ggtgggcatt gccattggcg ccgcgggggt gcccgcgctc ctcgatcggc gcggcgaacc
     3241 agaccgggcc gggcgcccgc tcgaggtcac cgaggtgggc tttgccgatg cggtggcggc
     3301 aatggccgtg ctagcgatgg gcgaggcggc cgaagcgcgc cctgccgcac tagtgcgcgg
     3361 cctcagctgg tcggcgcccg cgcgtccggc cgcggcgctg gtgcggccca agcccgacga
     3421 tatgttccga tgagcgaggc aatgtcccgc tttgtggcgc tgtcgggcgg cgtgggcggg
     3481 gccaagctcg ccttgggctt ggcgcacctg ctcggcgagc gcttgagcct gatcgtcaat
     3541 accggcgacg acttcgagca tttggggctc tacgtttcgc cagaccttga cacggcgctc
     3601 tatacgctcg ccgaacgcgt caatcctgcg accggttggg ggcgcagcgc cgaaacctgg
     3661 accttcatgg ctgcgctcga agagctgggc ggcccaacct ggttcaacct tggagacgga
     3721 gacctcgcca cccacgtcct gcgcagcgag cgcctcagag ccggtgcgac gctgaccgcg
     3781 gtgaccaccg agctggcgcg tcggctcggc gtcaagatcg agatcctgcc catgtgcgat
     3841 tctgagttgc gcaccgtgct ggagaccgat gccggcacc
//
LOCUS       DASTMT010000565         2102 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_5420477, whole genome shotgun sequence.
ACCESSION   DASTMT010000565 DASTMT010000000
VERSION     DASTMT010000565.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2102
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5420477"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            272..1504
                     /locus_tag="VFR00_12940"
     CDS             272..1504
                     /locus_tag="VFR00_12940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018455992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0160221.1"
                     /translation="MQPANADRGETSLRYPGWRLVVMCFVMALLCWGFGFYGHGFYLA
                     ELRRQHGWSTALISSASTACYLVSAVLVIFISDAIGRFGVRGCVLTGSTAFAATAAAL
                     PFLTEPWQLFAVYLVMALGWATMSVGAITNILGLWFEQKRGLAISLALNGASFSGVIV
                     VPFLVLITERRGFAEAMLVSALIIAALIVPLLALIPGGERARATPVLAASPPARVTWT
                     RSSALRTWAFWSVSAPFALAIFSQAGFLVHQIAFLEPTLGRNASGIAVAITTGMAIFG
                     RLTLGAFADRIDQRIASALSLISQAAALAMMAGSSERAVLMAAAALYGFSVGNVITFP
                     ALIVQREFDAAAFGMLVGLATGFSQFTYAFGPAVLGIVRDLTGGYWGPLWLCIALNLL
                     AAALVVRRPGSRQGTPVC"
     gene            1592..>2102
                     /locus_tag="VFR00_12945"
     CDS             1592..>2102
                     /locus_tag="VFR00_12945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020094903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0160222.1"
                     /translation="MTRSAQSLGAPTGKAESAYAWLRLLTAMLLGTIGGVGMWSFVVQ
                     LPVVQADFGVARADASLPYTMGMLGFAVGAVAFGRLVDRLGIIAPLVLSTLLLGFAYI
                     GSAFSANIWQLAIVHGCIGLGSSATFAPLIADISHWFTRRRGLAVSICATGSYLAGTF
                     WPPIVNQFVA"
BASE COUNT          257 a          727 c          678 g          440 t
ORIGIN      
        1 ttcttccact cgacccactt cgagcggcgc atgctgtttg gacgcgagcc tatggtggcc
       61 ctggcgacgg ccgcctagct gagcggcccc ccgcaccctg cggcgcccgc tgcggggcat
      121 gtgggcgccg ctgcgacgtc atggggtccc gggggctcct ggggcgccga tgcaggcgtg
      181 cgcttgggca aaccaacttg gacaaatgac cggctcggcg gcatgctggc cggcggccgc
      241 cgcctgccgg tggctttcgc ctttgaggac gatgcagccc gccaacgccg accgcggcga
      301 aacttccctt cgctatccag gctggcgcct ggtcgtcatg tgtttcgtca tggcactcct
      361 gtgctggggg tttggctttt atggccacgg cttctacctc gccgaactca ggcgacagca
      421 cggctggtcg acggcactga tctcgagcgc cagtacggcc tgctatctcg tcagcgccgt
      481 gctcgtcatc ttcatcagcg atgccatcgg ccgttttggc gtgcgcggct gcgtcctcac
      541 gggcagcacc gcctttgccg cgacggcggc ggccttgccc ttcctcaccg agccttggca
      601 gctgtttgcg gtctacctgg tgatggcact gggctgggcg acgatgagtg tcggcgccat
      661 caccaacatc ctgggcctct ggttcgagca aaagcgcggg cttgccatct cgctggcgct
      721 caatggcgcc agcttcagcg gggtgatcgt cgtccccttt ctcgtcctga tcacggaacg
      781 gcgagggttt gccgaggcca tgctcgtcag tgccctaatc atcgcagccc tgatcgttcc
      841 cctgttggca ctcatccccg gcggcgagcg ggcgcgcgca acgcccgtgc tggcggcgag
      901 cccgcccgcg cgcgtcactt ggactcgcag ctccgccctc agaacttggg cgttctggag
      961 cgtgtcggcc ccgtttgcgc tggcgatctt ttcgcaggcc ggttttcttg tgcaccaaat
     1021 cgccttcctg gagccaacgc tcggccgcaa cgcctccggc atcgccgtcg ccatcaccac
     1081 cggcatggcg attttcggcc ggctgacgct cggtgccttt gccgaccgca ttgaccagcg
     1141 catcgccagc gccctttccc ttatcagcca ggcggcggcg ttggcgatga tggccggctc
     1201 gagcgagcgg gcggtcctca tggccgccgc cgcgctctac ggcttttccg taggcaacgt
     1261 catcaccttc ccggctctga tcgtgcaacg cgagttcgat gccgccgcct tcggcatgct
     1321 ggtggggctg gcaacgggct tcagccagtt cacctacgcg ttcgggccag cggtgcttgg
     1381 catcgtgcgc gatttgaccg gcggctattg ggggccgcta tggttgtgca tcgccctcaa
     1441 cctgttggcc gccgccctgg tcgtgcgccg ccctgggtcg aggcagggaa cgcccgtctg
     1501 ctgagcgggg cccgtctacc gtaaaacgca gccctgcagc gcgagctggc cgcttgaccg
     1561 tggtcggcca agcccggatt gtgcccggcc catgaccagg tctgcccaat ccttaggcgc
     1621 cccgaccggc aaggcggagt ccgcctatgc ctggcttcgc cttctgactg cgatgctgct
     1681 cggcaccatc ggcggcgtcg gcatgtggtc gtttgtggtg cagttgccgg tcgtgcaggc
     1741 cgatttcggc gttgcccgcg ccgacgcctc gctgccctac accatgggca tgctgggttt
     1801 cgccgtgggc gcggttgcct tcggccgcct ggtcgatcgc cttggcatca tcgcccctct
     1861 ggtactgagt acactgcttc tgggctttgc ctatatcggg tcggccttca gcgccaatat
     1921 ctggcaattg gcgatcgtgc acggctgcat tggcttgggc agctcggcca cctttgcccc
     1981 cctgattgcc gacatctccc attggttcac gcggcggcgg ggtttggcgg tgtcgatctg
     2041 cgccaccggc agctacttgg cggggacgtt ctggccaccg attgtcaacc aattcgtcgc
     2101 ca
//
LOCUS       DASTMT010000566         2307 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_3773885, whole genome shotgun sequence.
ACCESSION   DASTMT010000566 DASTMT010000000
VERSION     DASTMT010000566.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2307)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2307)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2307
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3773885"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..627)
                     /locus_tag="VFR00_12950"
     CDS             complement(<1..627)
                     /locus_tag="VFR00_12950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012115304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0160223.1"
                     /translation="MPHFVSRRQFGGLAAASAALAAAAPVLAQQSNEIIKWRLTSSFP
                     KSLDTLFGAAATISRIVGEMTDGKFTIQTFAAGEIVPGLQVLDAVSNGTVECGHTYTG
                     YYIGKNPAFIFDGSLPFGLTPRMHNAWMMFGNGRKLMDEVYDSFNIVCTPAGQTGGQM
                     FGWFRKEIKSPKDFEGLKMRTAGFGGKVMAKLGAVPQQIAGGDIYPALE"
     gene            complement(772..1680)
                     /gene="hemF"
                     /locus_tag="VFR00_12955"
     CDS             complement(772..1680)
                     /gene="hemF"
                     /locus_tag="VFR00_12955"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HEU0160224.1"
                     /translation="MTGLDTTPEPYKTGARIWFEKLRDDLCAAFERLEVELPASAPLS
                     DRPAGQFAITPWQRQDHGGDPGGGGRMSLLKGRVFEKAGIHTSTVFGEFAPEFRKQIP
                     GAEHDPRFWASGLSLIAHPHNPHVPAAHMNTRFVVTSKWWFGGGADLTPLLEARRSQS
                     DPDTIAFHAAMQQACAPHALADYPRYKAWCEDYFYLPHRQEMRGVGGIFFDYLTPAEA
                     EGGWDAGLAFTQAVGRAFLAVYPELVRRNFALPWGASEREAQLVRRGRYVEFNLLYDR
                     GTVFGLKTGGNVEAILSSLPPVVTWP"
     gene            1981..>2307
                     /locus_tag="VFR00_12960"
     CDS             1981..>2307
                     /locus_tag="VFR00_12960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug ABC transporter ATP-binding protein"
                     /protein_id="HEU0160225.1"
                     /translation="MFRWFETRIEAFPEAPLERPPSTLFAYYVYFIRPVWPAFAALLI
                     AGLLGALIEVALMTFIGNLVDLMRAAASPAHFMADHGPILLWMGLVAVVARPLVSTVH
                     DLIKNQI"
BASE COUNT          376 a          749 c          749 g          433 t
ORIGIN      
        1 ctcgagcgcg ggatagatgt cgccgccggc gatctgttgc ggcaccgcgc cgagcttggc
       61 catcaccttg ccgccgaaac cggcggtgcg catcttcagc ccctcgaagt ccttcgggct
      121 tttgatctcc ttgcggaacc agccgaacat ctggccgccg gtctggccgg ccggcgtgca
      181 gacgatattg aagctgtcat agacctcgtc catcagcttg cggccgttgc cgaacatcat
      241 ccaggcgttg tgcatgcgcg gcgtcaggcc gaacggcagc gagccgtcga agatgaaggc
      301 cgggttcttg ccgatgtagt agccggtgta ggtgtgaccg cactcgaccg tcccgttcga
      361 taccgcatcg agcacctgca ggccgggcac gatctcgccg gcggcgaagg tctggatcgt
      421 gaacttgcca tccgtcattt cgccaacgat ccgcgagatg gtcgcggcgg cgccgaacag
      481 cgtgtcgagg ctcttgggaa agcttgaggt caagcgccac ttgatgattt cgttgctctg
      541 ctgagccagc accggggccg cggcggcgag cgccgcactg gcggcggcca gcccgccgaa
      601 ttgccggcga gacacgaaat gaggcattgc tatttccttc ccggttccag ttgttcctgc
      661 ggcagcctag cacggggtga acggacggtt gcaatggccg atcgctgcga tcgggctgtt
      721 tttctgcccg gccgcgccag cgacggtggc ggtgtcgcgc tgagcttgca ctcagggcca
      781 agtgacgacg ggcggcagcg aggacagaat ggcctccaca ttgccgcccg tcttcaagcc
      841 gaacaccgta ccgcggtcgt agagcagatt gaattcgacg tagcggccgc gccggaccag
      901 ctgcgcttcg cgctcgctgg ccccccaggg caaggcaaaa ttgcggcgca cgagctcggg
      961 gtaaacggcg aggaacgcgc gacccacagc ctgggtaaac gccagccccg cgtcccagcc
     1021 gccctcggcc tcggcggggg tcaagtagtc gaaaaagatg ccgcctacgc cgcgcatctc
     1081 ctggcgatgc ggcaggtaga agtagtcctc acaccacgcc ttgtagcggg ggtagtcggc
     1141 gagcgcatgc ggggcgcagg cctgctgcat ggccgcatga aaggcaatcg tgtcggggtc
     1201 gctttgcgag cgccgcgcct cgagcagggg ggtgaggtca gcgccgccgc caaaccacca
     1261 cttgctggtc accacaaagc gcgtgttcat gtgggcggca ggcacgtgtg gattgtgcgg
     1321 atgcgcgatg agcgacaggc ccgaggccca gaagcgcgga tcatgttcgg cacccgggat
     1381 ctgcttgcgg aattccgggg caaactcgcc aaacaccgtc gaggtgtgaa tgcccgcctt
     1441 ttcgaacacc cggcccttga gaagggacat gcggccgccg ccacccggat caccgccgtg
     1501 gtcctggcgt tgccagggcg tgatcgcgaa ctgaccggct gggcgatcgg acagcggcgc
     1561 ggacgcggga agctctacct cgagccgctc gaaggccgcg cagagatcat cgcgcagttt
     1621 ttcaaaccaa atacgcgcgc ccgtcttgta cggctcagga gttgtgtcga ggcctgtcat
     1681 tgctggctcc acgcgcgctt ggcagccgtc cgcacgcact ggtgcctcgt cgggctttgt
     1741 gctatgagct gccgcgctcg acgctacaac atcttgcggc agcaccgcca tgatcgatcg
     1801 catcgtgcct ttgcaccgct gaggtcgccc gcatcgctgc gcgccggccg atccttgcgt
     1861 gtgccgcagc gcctcttccc cgccagacgg tctggctgat cgtcgtctct tttcgctgct
     1921 atcgttgttc aagcccctct gcgccaagcc gtccccggcg ctgatctttg ggtaccgcaa
     1981 atgttccgct ggtttgaaac ccgcattgaa gctttcccgg aggccccgct ggagcgcccg
     2041 ccgagcacgc tctttgccta ctacgtctac tttatacgac cggtgtggcc ggcgtttgcc
     2101 gccttgctga tcgccggctt gttgggtgcg ctgatcgaag tggcgctgat gaccttcatc
     2161 ggcaatctgg tcgatctgat gcgagccgca gcatcacccg cccacttcat ggctgatcat
     2221 gggccgatct tgctgtggat gggcctggtg gccgtggttg cccggccact ggtgtcgacc
     2281 gtgcacgacc tcatcaagaa ccagatc
//
LOCUS       DASTMT010000567         8901 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_5421939, whole genome shotgun sequence.
ACCESSION   DASTMT010000567 DASTMT010000000
VERSION     DASTMT010000567.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 8901)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8901)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8901
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5421939"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(56..1045)
                     /locus_tag="VFR00_12965"
                     /pseudo
     CDS             complement(56..1045)
                     /locus_tag="VFR00_12965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008134766.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
     gene            complement(1051..2217)
                     /locus_tag="VFR00_12970"
     CDS             complement(1051..2217)
                     /locus_tag="VFR00_12970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012112017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HEU0160226.1"
                     /translation="MGPLAGLRIVEMAGIGPAPFAATLLADMGAELIRVDRRTATDLG
                     LPGREAKFNVLHRGRRSLAVDLKSERGREVVKRLVRKADGLLEGFRPGVMERLGLGPD
                     VLLALNPKLVFGRMTGFGQEGPMAQNAGHDINYISLAGVLHAIGRRNDEPPVPPLNLI
                     GDFGGGGMFLAFGVVCALLEAQKSGQGQIVDATMVDGAAYLMGPLFGLFAQGSWRDER
                     GVNVLDGGAPWYNVYRTKDGKWLSVGAIEQRFYAEFVMKLGLDPAQLPAQHDRQGWPV
                     LAQRFREVIATRTREEWEAVFTGSDACVAPVLSLAEVEHHPHNKARNTFLRRDGVLQP
                     APAPRFSRSEPEVGLSPRPLGADSDAVLGDWGFDAAEIAELRGAGIIGALAEER"
     gene            2380..4599
                     /locus_tag="VFR00_12975"
     CDS             2380..4599
                     /locus_tag="VFR00_12975"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU0160227.1"
                     /translation="MSQLHDLSGRLPALLQALQKRLLRASRLRLALGRRKIRALPPAR
                     ELPLEFDRFLRIGERLERGLEELKDIQWQIRENEARYRDLLDNQADVILRRDAQSRLT
                     FVNRAFCSVFGLEREDVLGRPFVHRVLAGEATLPLTPASALRHQRYVQEIDTAAGPRW
                     FEWEEHAVPGHEMSVAEVQCLGRDITEERRAEAELKEARKQAVAANRAKSRFLAAMSH
                     EIRTPMNGILGMTQLLSETELAPEQQTYARAIDQSARTLLILIDEILDFSKIEADKLQ
                     LNCADLALDACVQGVVELLAPKAYEKGIDIAWAVDPKLPRRLMGDELRLRQIVTNLVG
                     NAIKFTDAGGVLITVAPPVHGAKPPDGEVAVAIAVEDTGIGIPPEALATLFGEFEQAE
                     LAIERRQGGTGLGLAISRRLARAMAGDIQVTSRPGFGSTFVASVRLKRADEPLAARPL
                     RAKLVPAPHVLLALERPMERRALRLTLEGAGIPLEEAGHADAAELIVAAAGLGEPFTA
                     LLVDGCCGPEASARLLACARRAAGERSVRGIVVLDTSAKADFAEFRDAGFQAYLVRPA
                     RPHSVLTQLGAGFVHASGPAACANRPAEPSRFASTPRVLLVEDNDINALLARRMLEKA
                     GCETVLCGNGREAVEAVRRAIAGNDRGYDLILMDVHMPVLDGLEATRSIRALLNAVVD
                     PTPTSLPIVALTANAFAEDRRRCLEAGMDDYLAKPFDREDLYRLLERWCGGERTRAA"
     gene            complement(4603..6873)
                     /locus_tag="VFR00_12980"
     CDS             complement(4603..6873)
                     /locus_tag="VFR00_12980"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012706619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004470 - malic enzyme activity [Evidence
                     IEA];
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HEU0160228.1"
                     /translation="MSRKFAAAHFTAQEALQFHSQGKPGKLEISPTKPLTTQRDLSLA
                     YSPGVAVPVQAIAQDENLAYDYTNKANLVAVVSNGTAILGLGNLGAQAAKPVMEGKAA
                     LFKRFADIDAIDLLVDTPDADAVINCVRYLGPSFGGINLEDIKAPDCFIIEEKLKEYL
                     NVPVFHDDQHGTAIIAAAGLLNALELTGRHIETFKLVVNGAGAAGIACLELLTAMGMP
                     RRNVVFCDTKGVIYQGRKEGMNQWKSGYAANTKARTLADALEGADVFFGLSAAGAVTQ
                     AMVKSMAEKPIIFAMANPDPEITPEEVMEVRPDAIVATGRSDYPNQINNVLGFPFIFR
                     GALDVQASTINEAMKIAAAKALAELAQEDVPDQVAAAFLGRQVKYGPDYIIPAPFDPR
                     LITHVPPAVAKAAMDTGVARRPIVDMEAYVGRLSARLDPVAGWLNSIFAQVRAAPKRI
                     VFAEGEQPQIARAANAFHNAGFGRPILIGRGAPIREAFNLAGIGLTDVIEMHDTSLDE
                     RRQEYAEFLYGRLQRKGYLLRDCQRLVNNDRNVFAACMVAMGHADGMVTGVTRNWYTA
                     YEDVRKVLDARPRRRVIGVSVALIRGRVVLVADVSVHDMPDGTQLADIAEEAVRVARR
                     LAIEPRVALLAYSTFGHPRGERSDRVREAVAILDKRQVDFEYDGDMAADVALNREALK
                     QYPFCRLSDTANVLVMPAFHSASISTKMLKELGGATLIGPVLGGLTHSVQICPLNSMD
                     NEVVNMAALAAYDVNT"
     gene            7015..>8901
                     /gene="mutS"
                     /locus_tag="VFR00_12985"
     CDS             7015..>8901
                     /gene="mutS"
                     /locus_tag="VFR00_12985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEU0160229.1"
                     /translation="MADPRKTSKAAAGAKLRAPRAVWAAALPPPALENEPSSHGITPS
                     MAQFLKIKSAHPDCLLFYRMGDFYELFFQDAVAAAQALGIVLTKRGKHLGEDIPMCGV
                     PIHRADEYLFRLIKQGFRVAVCEQLEDPAQARRRGAKAVVQRDVVRVVTPGTLTEDTL
                     LDAKTRNYLTAMCSLAHGSGPERRIALASLEISTGECEIGEVSTADVAGDIMRLSPSE
                     VLASDQALQDSALRKCLDGVRAAITPVPAASFDSLAGERLLKQQLGVADLAAFGDFTR
                     AELAAAGALLKYVELTQLGQKVPLRVPRRTGPEDRLLIDAATRTSLELLRSLAGDKRG
                     SLLGAIDRTLTGAGARELASRLAGPLRDPAQIAARLDVVGFLNERDGLREDLRRALRS
                     APDIARALARLALQRGGPRDLAAVRDGLLTAGGCAALIEAAAAPVGLPRNLAAIVRAL
                     CACSASLAAELTAALADELPPHRREGGFVRQGFRPELDEARALRDGSRHTLAELEAKY
                     AADTGVKSLRVRHNNILGFYLEAPAVAAKPLLSEPLAQRFKHRQTMAGAVRFTTEELI
                     EIQTRIVSAAERALALEQEVFAALAAAIEREHARLLGLAAALAELDCEAGLAEVAAEQ
                     AYARP"
BASE COUNT         1489 a         2952 c         2899 g         1561 t
ORIGIN      
        1 actccttggc atagcccatg ccgcccaacg tcaggactgc cgtctcgcag gcctaaaatc
       61 ccgcctccgc cgccaggtac ttggctgcat tggcttcgat gccgcaggcc tcgcccgcat
      121 cgtaaagccg agccgccttc agcatcatga ggttagccgc ttcaagcgcc atccagcact
      181 tggccagcgg atgttggatc gcctggtttt gtccgatcgg gcggccgaag acgatgcgct
      241 ccttggcata acggctggca cgggcgagcg ccgtccgacc aatgccgatc gcttcggcgg
      301 ccaccagaat gcgttccggg ttcatgccat gcaggatgta ctcgaagcct cggccttcct
      361 cgccgatcag atcctccgcc ggcaccggca tgccatcgaa aaagagctgg ttggagtcga
      421 cagctttgcg gcccatcttg tcgatgacgc gggcctccac ccgactgcgt tcgagcttgg
      481 tataaaacag ggagaggcca ccggtgcgct tcttcacctc ttcgagcggc gtcgtgcgcg
      541 ccaaaagcag cacgcgctcg gccacctgcg cggtcgagat ccagatcttc tgcccatgga
      601 cgaggtagcc gtcgttgctg cgcactgcgc gcgtggtgag cctggtcgta tcgagaccga
      661 cgttcggctc ggtcaccgcg aagcaagcct tttcctctcc tcgcaccagt ggcggcaaaa
      721 agcgctgctt ttgcgcttcc gtcccaaaca ccaccaccgg attgagaccg aaaatgttca
      781 tatgcacggc cgaggcgccg gaaaaggcgg cgcccgactc ggcaatcgcc tgcatcatca
      841 cggcggcttc cgtgatgccg agcccggatc cgccgtacgc ctccggtatg gcgatgccga
      901 gccagccggc ctcggccagc gcgcggtgaa agtcgtgcgg aaagccaccg tctccatctc
      961 tggccaacca atattcgtcg ccaaatttgc tgcaaatcct cgacacggaa gcgcggattt
     1021 cctgctgggc cggggtcaaa tccatcgccc tcatcgctcc tctgccagcg ccccaattat
     1081 cccggcgcca cgcaattctg caatttccgc tgcgtcgaag ccccaatcgc cgagcacggc
     1141 atcgctatct gccccaagcg gacgcgggga cagccctacc tctggttcgc tgcggctgaa
     1201 gcggggcgca ggtgctggtt gcaggacccc gtcgcgccga aggaaggtgt tgcgcgcctt
     1261 gttgtgcgga tggtgttcca cctccgcaag cgagagcacc ggtgcaacgc aagcatcgct
     1321 gccggtaaag acggcttccc attcctcgcg cgtgcgcgtg gcaatcactt cacggaaacg
     1381 ctgcgccagc acgggccagc cctgcctgtc gtgctgggcg ggaagttgcg ctggatcgag
     1441 gcccaacttc atcacgaact ccgcgtaaaa gcgctgctcg atggcgccga cggagagcca
     1501 cttgccgtct ttggtccgat acacgttata ccagggcgcg ccaccatcca gcacgttgac
     1561 gccccgttca tcgcgccagg agccctgcgc gaacagcccg aacagcgggc ccatcaaata
     1621 ggcggcgcca tccaccatgg tggcgtcgac gatctgacct tgcccggact tctgcgcttc
     1681 gagcaatgcg cacaccacgc caaaggcgag aaacatccca ccccctccaa agtcgccgat
     1741 caggttgagc gggggcacgg gaggctcatc gttgcggcgg ccaatcgcat gcagcacgcc
     1801 ggcaagcgaa atgtagttga tgtcgtgccc ggcattttgc gccatcggcc cctcttggcc
     1861 gaaacccgtc atgcggccga aaacgagttt cggattgagc gcgagcaaga cgtccggacc
     1921 aagccccaac cgctccatca cgcctggccg gaagccctcc agcaggccgt ccgccttgcg
     1981 aaccaggcgt ttcaccacct cccggccccg ctcgcttttc aggtcgacgg ctagcgagcg
     2041 ccggccgcgg tgcagcacat tgaacttggc ttcccggccg ggcaggccaa ggtctgtcgc
     2101 cgtgcgtcga tcgacgcgta tcagttcggc gcccatgtcg gccagcaggg tcgctgcaaa
     2161 cggcgctggg ccgatcccgg ccatctcgac aatgcgcaaa ccggccagcg gacccatcgt
     2221 ggttacctct tggttggccg ctgtgccgga acgcaccgtg agacgggcga gagcgcgtgc
     2281 ggcagcggcg agcgtgaacg atgcgggtaa acttttctca atctggatgt ggttctcatg
     2341 cacgacaagc cgtcaacgat aaatgatcgg gcgtcaaggg tgtctcagct gcacgacctg
     2401 tcgggccggc tgccggcttt gctgcaagcg ctgcaaaagc gattgctgcg tgccagccgc
     2461 ctgcgtctcg cgctcggacg gcgcaaaatc cgcgcgctgc cgcccgcgcg cgagcttccc
     2521 cttgaattcg accgcttcct gcgcatcggc gagcgcctcg aacgcggctt ggaggagctc
     2581 aaggatatcc aatggcaaat ccgcgagaac gaagcacgct atcgggatct gctggataat
     2641 caggccgatg tcatcctgcg ccgcgacgcc cagagccgcc tgaccttcgt gaaccgggcg
     2701 ttttgctcgg tgttcgggct ggagcgcgaa gatgtgctcg gccgcccttt tgtccatcgg
     2761 gtactggccg gcgaggcgac gctgccgctc accccggcca gcgccttgcg gcaccagcgt
     2821 tacgtgcagg agatcgatac ggcggcgggg ccgcgctggt ttgagtggga ggaacacgcc
     2881 gtccccggcc acgagatgtc ggtcgccgag gtgcagtgtc tcggccgcga catcaccgag
     2941 gaacgccgtg ccgaagcgga actcaaggaa gcacgcaagc aagccgttgc ggccaatcgc
     3001 gccaagtcac gctttctggc cgccatgagc catgagatcc gcacgccgat gaacggcatc
     3061 ctcggcatga cccagttgct aagcgagacc gagctggcgc ccgagcaaca gacctacgct
     3121 cgcgccatcg atcaatcggc ccgcaccttg ctcattctca tcgatgaaat cctcgacttc
     3181 tccaaaatcg aagccgacaa gctgcagctc aattgcgcgg acctggcgct cgatgcgtgc
     3241 gtgcagggcg tcgtcgagtt gttggcgccg aaggcctacg agaaggggat cgatattgca
     3301 tgggctgtcg atccgaaatt gccccgtcgt ctgatgggcg acgagctgcg gctgcgccag
     3361 atcgtcacca acctggtcgg caatgccatc aagttcaccg atgcgggcgg ggtgttgatc
     3421 accgttgccc cgccggtgca tggcgccaag ccgccagacg gcgaggtcgc ggtggcgatc
     3481 gcggttgaag atacgggcat cggcatcccg cccgaggcgc tcgccaccct gttcggtgaa
     3541 ttcgagcagg ccgagctggc cattgagcgc cgccagggcg gaaccggact gggacttgca
     3601 atttcgcgac gcttggcgcg cgccatggcc ggcgacattc aggttaccag tcgtcctggc
     3661 ttcggctcta cctttgttgc cagcgtgcgc ctgaaacgcg ccgatgagcc gctcgccgcg
     3721 cgccccctgc gggcgaagtt ggtgccggcg ccgcatgtgt tgttggcgct cgagcggccg
     3781 atggaacgcc gcgccctgcg cctgacgctc gaaggcgccg gcattccgct cgaagaagcg
     3841 ggccacgccg atgcggcgga gcttattgtc gccgccgcgg gcctcggcga gcccttcacc
     3901 gccctgttgg tcgacggttg ctgcggcccg gaggcgtccg ctcggctatt ggcttgtgcg
     3961 cgacgggcgg ccggggagcg gtcggtgagg ggcatcgtcg tcttggatac ttccgccaag
     4021 gctgattttg ccgagtttcg cgacgccggt tttcaggcct atctggtgcg cccggccagg
     4081 ccgcactcgg tcttgaccca acttggcgcc ggcttcgtgc acgccagcgg gccggcggcc
     4141 tgcgccaatc gcccggcgga gccaagtcgg tttgcctcaa cgccgcgtgt cttgctcgtc
     4201 gaagacaatg acatcaatgc gctgctggcg cggcgcatgc tggagaaggc aggctgcgag
     4261 accgtcctgt gcggcaacgg gcgcgaggcg gtggaggccg tgcggcgcgc gatcgccggc
     4321 aacgatcgag gctacgatct catcctgatg gacgtgcata tgcccgtgct ggatgggctg
     4381 gaggcgacgc gttcgatcag agcgctgctg aatgccgtgg tcgatccgac gccgacgtcg
     4441 ctgccgattg tcgctttgac cgccaatgcc tttgctgagg atcgccgccg ctgcctggag
     4501 gcaggaatgg acgactactt ggcaaaaccc ttcgaccgcg aggacctcta ccgcctattg
     4561 gagcgttggt gcggcggcga gcgtacgcgc gctgcctgag gctcaggtgt tgacgtcata
     4621 ggccgcgagc gccgccatgt tcacgacctc attgtccatg gagttcaacg ggcagatttg
     4681 cacggaatgc gtcaggcccc ccagcaccgg cccgatcagc gtggcgcccc ccagctcctt
     4741 caacatcttg gtcgatatcg aggcggagtg gaaggccggc atcaccagga cgttggcggt
     4801 gtcggacagg cggcagaacg gatattgctt gagcgcctcg cgattgagcg caacgtcggc
     4861 ggccatgtcg ccatcgtact cgaaatcgac ttggcgcttg tcgaggatcg cgaccgcctc
     4921 gcgtacgcgg tcggaccgct cgccgcgggg atgcccgaag gtcgaatagg ccagcagcgc
     4981 gacgcgcggc tcgatcgcca gccggcgggc aacgcgcaca gcctcctcgg caatatcggc
     5041 cagctgagtg ccgtccggca tgtcgtgcac gctgacgtcg gccaccagca cgacgcggcc
     5101 gcgaatgagc gcgaccgaga cgccgatcac gcgccgccgc ggtctggcgt ccagcacttt
     5161 gcgcacatct tcataggcgg tgtaccaatt gcgcgtgacg cccgtgacca tcccgtcggc
     5221 atgccccatc gctaccatgc acgcggcgaa tacgttgcgg tcgttgttga cgagccgctg
     5281 gcagtcgcgc agcaaatagc ccttgcgctg caggcggccg tagaggaact ccgcatactc
     5341 ctgacggcgt tcgtctagac tggtgtcgtg catctcgatc acatcggtca ggccgatgcc
     5401 cgcaagattg aaggcctcac ggatgggcgc accgcgcccg atcaggatgg gtcggccaaa
     5461 accggcgttg tggaaggcgt tcgccgcccg tgcaatttgc ggctgttcgc cttcggcaaa
     5521 aacgatgcgc ttgggtgcgg cgcgcacttg cgcaaaaatg ctgttcagcc aacccgccac
     5581 cggatcgagg cgcgccgaca agcgaccgac gtaggcctcc atatcgacga ttggccggcg
     5641 cgccacgccc gtgtccatgg ccgccttggc caccgcgggc ggcacgtgcg tgatgagcct
     5701 ggggtcgaag ggtgccggga tgatgtagtc ggggccatat ttcacctgcc ggccgaggaa
     5761 ggccgccgcc acttggtcgg gaacatcctc ctgggcaagc tccgccaatg ccttcgccgc
     5821 ggcgatcttc atcgcctcgt tgatggtgga ggcctgcacg tcgagcgcac cgcgaaagat
     5881 gaacgggaag cccaacacgt tgttgatctg gttggggtaa tcggaacgcc cggtggccac
     5941 gatggcgtcg ggccgcactt ccatcacctc ctcaggagta atttcgggat cgggattggc
     6001 catggcgaag atgatcggct tctccgccat cgatttgacc atggcctgcg tcaccgcacc
     6061 cgccgccgaa aggccgaaga acacgtcggc gccctccagt gcatcggcca gcgtgcgtgc
     6121 cttggtgttg gcggcatagc ccgacttcca ctggttcatg ccttccttgc ggccctggta
     6181 gatcacgccc ttggtgtcgc agaacaccac gtttcggcgg ggcatgccca tggccgtcag
     6241 tagctccagg caggcaatgc ccgcggcacc agcgccattg acgacgagct tgaacgtttc
     6301 gatgtggcgg cccgtcagct cgagcgcgtt gagcaggccg gccgcggcaa tgatcgctgt
     6361 gccgtgctgg tcgtcgtgga acaccgggac gttgagatac tccttgagct tctcttcgat
     6421 gatgaagcag tcgggagcct tgatgtcttc caggttgatg ccgccgaagc tcgggccaag
     6481 atagcgcacg caattgatga cggcgtccgc atcgggcgtg tcgacgagca ggtcgatggc
     6541 atcgatgtcg gcgaagcgct tgaacagggc cgccttgccc tccatcaccg gcttggccgc
     6601 ctgcgcgcca agattgccga gaccgagaat cgccgtgccg ttggatacga ccgcgaccag
     6661 gttcgccttg ttggtatagt cataggcaag gttttcatcc tgggcgatgg cctgcaccgg
     6721 caccgccacg ccgggggaat aggccaaaga cagatcacgc tgtgttgtca ggggcttggt
     6781 gggagagatc tcgagcttgc cgggctttcc ctggctatgg aactgtagtg cctcctgcgc
     6841 cgtgaagtgc gcggcggcaa actttctcga catgagccac tcccaagaga tcgctgccac
     6901 ccgacgatag agcgctggcc tgggtcacac aatggcgccg gacaagatgg cgctcctgtt
     6961 ccctcgccgg cacggaaggg ctatgccttg gccggcacgg gttatggcgg agcaatggca
     7021 gatcctcgca agacatccaa ggcggctgcc ggcgcaaagc ttcgggcgcc gcgcgcggtc
     7081 tgggcagcgg cgctgccgcc cccggctctc gaaaatgagc cttcctccca cggcatcacg
     7141 ccgtccatgg cgcagtttct gaagatcaag tccgcccatc ccgactgcct cctgttctac
     7201 cgcatgggcg atttctacga gctcttcttc caggatgccg tcgccgctgc ccaggcgctc
     7261 ggaatcgtgc tgaccaagcg cggcaagcac ctgggcgagg atattcccat gtgtggggta
     7321 ccgatccacc gtgccgatga gtatcttttt cgtttgatca agcagggttt tcgcgtcgcc
     7381 gtctgcgagc aactcgaaga cccggcgcag gccaggcggc ggggtgccaa ggcggtggtg
     7441 cagcgcgatg tcgtgcgcgt ggtgacgccg ggcacgctga ccgaggatac tctgctcgac
     7501 gccaagacgc gcaactacct gacggcgatg tgctcactag cgcacggctc gggaccggag
     7561 cgccgcatcg cgctggcctc gctggagatt tccacgggcg agtgcgaaat cggcgaggtg
     7621 tcgacggccg acgtcgccgg cgacatcatg cgcttgtcgc cgagtgaggt gcttgccagc
     7681 gatcaggcgc tgcaggacag cgcactgcgc aaatgtctcg atggggtccg cgcggccata
     7741 acgccggtgc cggccgcctc cttcgatagc ttggccggcg agcggctctt gaagcagcag
     7801 ctcggcgtcg ccgacctggc ggctttcggc gacttcaccc gcgccgaact ggcggcggcc
     7861 ggggcgctct tgaagtacgt cgaactcacg cagctcgggc aaaaggtgcc cttgcgtgtg
     7921 ccgcgccgca ccgggcccga agatcggttg ctgatcgacg ccgccacgcg cacgagcctt
     7981 gagctgttgc gctcgctggc gggcgacaag cgcggtagcc tgctgggcgc catcgaccgg
     8041 acgctgaccg gtgccggcgc acgcgagctg gcaagtcgct tggcgggtcc gctcagagac
     8101 cccgcccaaa tcgccgcgcg cctcgatgtg gttggctttc tcaacgagcg cgacggcctg
     8161 cgggaggatc tgcgccgggc gctgcgttcg gcgccggaca tcgcgcgtgc gctggcccgc
     8221 ctggctctgc aacgcggcgg tccgcgcgat ctggcggcgg tgcgcgatgg gctgctgacg
     8281 gcgggcggct gcgcggctct catcgaggcc gccgccgcgc ccgtcggcct gccgcgcaat
     8341 ttggcggcaa tcgtgcgcgc cctttgtgcc tgttccgcca gcctcgcagc cgagctgacc
     8401 gcggcccttg ccgacgagct gccaccgcac cgccgcgagg gcggctttgt gcgccagggc
     8461 tttcgccccg agctcgacga agcgcgtgcg ctgcgcgatg gcagtcggca cacgctggcc
     8521 gagctggagg ccaaatacgc ggccgatacg ggggtcaagt cgctgcgcgt ccggcacaac
     8581 aatatcctcg gtttttacct cgaggcgccc gcggttgccg ccaagccgct gctgagcgaa
     8641 cccttggcgc agcgtttcaa acaccgccag accatggcgg gcgccgtgcg cttcaccacc
     8701 gaggagttga tcgagatcca aacgcgcatc gtctccgccg ccgaacgagc cctggctctg
     8761 gagcaggagg tcttcgccgc actggcggca gcgatcgagc gcgagcatgc gcgacttctc
     8821 ggtctggcag cggccctggc cgagctcgac tgcgaggcgg gccttgccga ggttgccgcc
     8881 gagcaagcct acgcgcgacc c
//
LOCUS       DASTMT010000568         5503 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_4441646, whole genome shotgun sequence.
ACCESSION   DASTMT010000568 DASTMT010000000
VERSION     DASTMT010000568.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 5503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5503
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4441646"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..639
                     /locus_tag="VFR00_12990"
     CDS             <1..639
                     /locus_tag="VFR00_12990"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEU0160230.1"
                     /translation="NVLTGFNVDMARAVCLELAAACDIQVRPWSELIPALRRGEADAL
                     IASHAISQTMLKVVDVSDRYYHTPARFAGPRSANRLEITPDGLEGRKIAVAKGTAHEA
                     YLRAFFSDSSIRAFETPELARDALITGAVDLLFDDGISLVFWLNGTASKGCCEFKGGP
                     FGEPKYFGDGIGVVVGREDQQLKSLINAALKHIRTTGRYEELLLRYFPLRAF"
     gene            complement(646..1608)
                     /locus_tag="VFR00_12995"
     CDS             complement(646..1608)
                     /locus_tag="VFR00_12995"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEU0160231.1"
                     /translation="MDIQDMRIFARVAALQNLSAVGAELGLTPGTISKRIQALEDQLS
                     ARLFDRTTRSIRITEEGSTFLVHVERILAEIEVACASVDDRVSKPKGTLKIAAPAFLG
                     RRYVAPALCQFMRSYPQIDVQVDLQDRPVNLQEDGYDVAIRTGTLSNSTLIAKRLAPD
                     RHIIAASPAYLARRGRPTQPEDLVRHDCLILGDRVQWPFSRNGLETSVRVQGPIRSND
                     GELLCQAAQDGLGLIRASELVVLSELRSGKLTQVLADYEVGSNAAVWALYPSSKHLLP
                     RMRALLDFLGVWFRDLASGQNGRPALTASDLAAVAEVHPPAPAG"
     gene            complement(1810..4425)
                     /locus_tag="VFR00_13000"
     CDS             complement(1810..4425)
                     /locus_tag="VFR00_13000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013945704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02302 family protein"
                     /protein_id="HEU0160232.1"
                     /translation="MAAPQTPRSSLERSLERKVALSRWALLFEALWPRVWVLIGLVGL
                     FLAASLVGLWARLDELTHKVVLGLFGLALLAALLALARVRWPTRAEAIRRVEGASGVK
                     HRPASSYEDTLTLNAEDARTAALWRVHRQRLAQLLGKLRVGRPAPRTDRYDPFAVRAL
                     LLLAVLTMLFAAGDSTSDRLWAAFRFGPLAKGAEARLDAWVTPPAYTGKPPIMLADGS
                     HGGWQQSERPAGAYDIPDKSLLVIRASGAGVGALTLEAPAGANGGTQRLEAPAPAKVG
                     DVAEIRTEIRWSGPVTVSSSGTPMATWTFQVTPDLPPKISLLKEPERTPRGGLKLFFK
                     VEDDYGVVSAETRIRRLTPKQDTTTTAWARAGAKKGPRPPLERPPTLALRLPKAYPKQ
                     AEGQSFHEIGDHPWAGMKVELTLVAKDLAGQVGRSDTIELVLPERRFSKPLARAVVEQ
                     RRRLVEDPRDRQQVVRALDALTLEPEGFIEDLQVYLGLRSAYWRLQREPSRAGRNSVI
                     AQLWDVALRIEDGNLTDAERALRAAQERLSKALEEGASDEEIQRLMQELRQALAQFLE
                     QLQKQAENQPPMQGMDRNNQFMTPQDLDQLLRNLENMARSGNRDMAQQMLSQLRDLLD
                     RLQSGRMADQGQSRQFGKMMDEFGNIIGRQQQLLDDTFGQQRQQGQQGQQGQRGQRGQ
                     QGQRGQQGQQGQRGQRGQQGQQGQQGEGPGDGDEFGGLGDRQRELRDMLGRLQRGMRE
                     FGLNTPEQFGGAGESMERAERALRNGDLDGAAQEESRALEQLRQGAREMAQQMLNQMP
                     SRFGLNDNNGELDPLGRPPQRTDGPDPGVGVKVPDQIDVQRAREILEELRRRLGEPTR
                     PPIELEYLERLLKLF"
     gene            4938..5255
                     /locus_tag="VFR00_13005"
     CDS             4938..5255
                     /locus_tag="VFR00_13005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009763146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEU0160233.1"
                     /translation="MGSIRVNDHNGRTHVLEAVEGWRVMELIRDHGLPIEAICGGACA
                     CATCHVHVAPAWAERLSPMREDEEGLLDALPQAADTSRLSCQIIWDSDLDGLELTLVG
                     DGA"
     gene            5252..>5503
                     /locus_tag="VFR00_13010"
     CDS             5252..>5503
                     /locus_tag="VFR00_13010"
                     /inference="COORDINATES: protein motif:HMM:NF024638.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU0160234.1"
                     /translation="MKYFPLFTRLDGAEVLVAGGGEAALQKVRLLLKTTAHITLMTAS
                     LHPELRALVDAGALTHVPRLPGPADLERQSLVYAATGDRT"
BASE COUNT          864 a         1887 c         1827 g          925 t
ORIGIN      
        1 aacgtgctga ccggcttcaa cgtcgacatg gcgcgcgccg tttgtttgga gctcgccgcg
       61 gcctgcgaca tccaggtgcg accgtggtcg gagttgatac ccgcgctcag gcgcggggag
      121 gccgacgccc tcattgcctc acacgcgatt agccagacca tgctcaaggt cgtcgatgtg
      181 agcgatcgct attaccacac gcccgctcgc ttcgccggcc cgcgctcggc gaaccgcctc
      241 gagatcaccc ccgacgggct agagggtcgc aagattgccg tcgccaaggg caccgcgcac
      301 gaggcctatc tgcgggcctt tttctccgac agttcgatcc gtgccttcga gacccccgaa
      361 cttgcgcgcg acgccttgat cacgggcgcc gtcgatctgc tgttcgacga tggcatcagc
      421 ctggtgtttt ggttgaacgg cacggcctcg aagggctgct gcgaattcaa gggcggcccc
      481 ttcggcgagc ccaagtactt cggcgacggc atcggtgtgg tggtgggacg cgaggatcag
      541 cagctgaaga gcttgatcaa cgccgcgctc aagcatattc gcaccaccgg gcgctacgaa
      601 gaattgctgc tgcgctattt tccgttacgc gcgttttagc cggcgtcagc cggccggggc
      661 cggcgggtgc acctcggcca ccgccgcgag gtcgcttgcc gtcagcgcgg ggcgaccgtt
      721 ttggccgctg gcaaggtcgc gaaaccagac gccgagaaaa tcgagcagcg cgcgcatgcg
      781 cggcagcaga tgcttggagc tcggatacag ggcccagacc gcggcgttgc tgccgacctc
      841 gtagtccgcc agcacctgcg tcagcttgcc tgagcgcagc tccgacaaca cgacgagctc
      901 ggaggcacgg atcaacccca atccgtcctg tgcggcctga cacaaaagct ccccgtcgtt
      961 ggagcggatc ggtccttgta cgcgcaccga cgtctccagc ccgttcctgc tgaagggcca
     1021 ctgcacgcgg tcgccaagaa tgaggcagtc gtggcggacg agatcctccg gctgcgtggg
     1081 ccgcccacgg cgggcgagat aggcgggcga ggccgcgatg atgtgccggt cgggcgccag
     1141 ccgcttggcg atcagcgtcg agttcgaaag ggtgccggtg cgaatggcaa catcatagcc
     1201 atcctcctgc aagttcaccg gccggtcctg cagatcgacc tgcacgtcga tttgcggata
     1261 gctgcgcatg aactggcaca gcgccggggc gacgtagcgg cgcccgagga aggccggggc
     1321 ggcaatcttg agcgttccct tgggcttgct gacccgatca tcgacgctgg cgcaggccac
     1381 ctcgatctcg gccaggatcc gctcgacatg aaccaggaag gtcgaccctt cctccgtgat
     1441 gcggatgctg cgcgtggtgc ggtcgaacag tcgggccgag agctggtcct ctaaggcctg
     1501 aatgcgtttg gagatggtcc ccggtgttag gccgagctcg gcgccgacgg cggacaagtt
     1561 ttgcaaagcc gccacgcgcg caaaaatgcg catatcctgg atgtccatca ccgctccccg
     1621 acgccggccg cacgttattg gttatttttc cccacccgat cgcgcggttc cggcggcttc
     1681 aacgcaagcc acgcaggacg tgcactctag cgagcaagtc ccgggtattc aaataagaat
     1741 tgtgctttgg ccatccccca aatggggggc ctttgcggtc cgcgcgcgcc caagcgcggc
     1801 agcctcggcc tagaacaact tgagcaggcg ttcgagatac tcaagctcga tcggcgggcg
     1861 cgtcggctcg cccagccgcc gccgcaattc ctccaggatc tctcgcgcgc gctgcacatc
     1921 gatttgatcg ggcaccttga cgccgacgcc gggatcgggg ccatcggtgc gctgcggtgg
     1981 acgcccaagc ggatcgagct cgccattgtt gtcgttgagg ccaaagcgcg acggcatttg
     2041 gttcagcatc tgctgggcca tctcgcgtgc gccttgccgc agctgctcca gggcgcggct
     2101 ttcctcctgc gcggcgccgt cgagatcgcc gttgcgcaag gcgcgctcgg cccgttccat
     2161 ggattcgccc gcgccgccga actgctcggg cgtattgagg ccgaactcgc gcatgccccg
     2221 ctgcagtctg cccaacatgt cgcgcagctc gcgctggcgg tcgccaaggc cgccgaactc
     2281 gtcgccgtcg cccggaccct cgccctgctg cccctgctgg ccctgttgac cgcgctggcc
     2341 gcgctgaccc tgttggccct gctggcctct ctggccctgc tgaccgcgct gccctctctg
     2401 tccctgctga ccctgctgtc cctgctggcg ctgctggccg aaggtatcgt cgagcagctg
     2461 ctgctggcgt ccgatgatgt tgccaaactc atccatcatc ttgccgaatt gccggctttg
     2521 accctggtcg gccatgcggc cggactgcag gcgatcgagt aggtcgcgca gctgggagag
     2581 catttgctgg gccatatcgc gattgcccga gcgcgccatg ttctccaaat tgcgcaggag
     2641 ctggtcgagg tcctgcggcg tcatgaattg gttgttgcgg tccatgccct gcatcggggg
     2701 ctggttttcc gcctgtttct gcagctgttc caggaactgg gccaacgcct gccgcagctc
     2761 ctgcatcagg cgctgaatct cttcgtccga ggcaccctct tcgagcgcct tggagaggcg
     2821 ctcctgagcg gcgcgcaggg cgcgctcggc gtcggtgaga ttgccgtcct cgatgcgcag
     2881 ggcaacgtcc cagagctggg cgatcacgct attgcgcccg gcgcggctgg gttcgcgctg
     2941 caggcgccag taggccgagc gcagtccaag gtagacctgc agatcctcga taaagccctc
     3001 aggctccaag gtgagcgcat cgagcgcgcg cacgacctgc tggcgatcgc gcgggtcctc
     3061 gaccaggcga cggcgctgct ccaccacggc gcgcgccagc ggcttgctga agcgccgttc
     3121 gggcaacacc agttcgatgg tgtcgctgcg cccgacctgg cccgcgagat ccttggcaac
     3181 gagcgtgagc tcaaccttca tacccgccca gggatgatcg ccgatctcat ggaagctctg
     3241 gccctcggcc tgcttggggt acgccttcgg tagccgcagc gccagcgtcg gcgggcgctc
     3301 gagcggcggc cgcggcccct ttttggcgcc ggcccgcgcc caggcggtgg tggtggtgtc
     3361 ctgcttgggg gtcaagcgcc ggatgcgcgt ttcggcggag actacgccgt agtcatcctc
     3421 gaccttgaag aacagcttga ggccgccgcg cggggtgcgc tcgggctcct taagcaggct
     3481 gattttgggc ggcaggtccg gcgtcacctg gaaggtccag gttgccatcg gcgtgcccga
     3541 actcgacacc gtcaccggcc ccgaccagcg gatctccgtg cggatttcgg caacgtcgcc
     3601 caccttggcg ggggcgggag cctcaaggcg ttgcgtcccg ccattggcgc ccgccggcgc
     3661 ctcaagcgtc agggccccca cgccggcccc gctggcccgg atcaccaaca ggctcttgtc
     3721 gggaatgtca taggcgcccg cgggccgttc cgattgctgc cagccgccat gcgacccgtc
     3781 ggccagcatg atgggaggct tgccggtgta ggccggcggc gtcacccagg cgtcgaggcg
     3841 cgcctcggcg cccttggcca gcggcccgaa gcggaaggct gcccacagcc ggtcggacgt
     3901 actgtcgccg gcggcaaaga gcatggtgag aaccgcgagc agcaacaaag cgcgcacggc
     3961 aaaaggatca tagcggtcgg tccgcggcgc cggtcgaccc acccgcagct tgcccaatag
     4021 ctgggccagg cgttgccgat gcacgcgcca cagggccgcc gtgcgggcat cctccgcatt
     4081 gagcgtcagg gtgtcctcgt aggaggaggc gggccggtgt ttgacgccgg aggccccctc
     4141 tacgcgccgg atcgcctcgg cacgcgtcgg ccagcgcact cgcgccaggg ccagcaacgc
     4201 cgccagcagc gccaggccaa agagccccag cacgaccttg tgggtgagct cgtccaagcg
     4261 ggcccacagc cccaccaggg acgccgccag gaacaggccg accaggccga tcagcaccca
     4321 gacgcgcggc cagagcgctt cgaacagcaa ggcccaccgg ctgagcgcga ccttgcgctc
     4381 caagctgcgt tcgaggcttg atcgaggtgt ctgtggtgcc gccatgggct cactctacca
     4441 cgctcgaggc gagcgcgctc aacacattaa cggggatgtt tgcgtgaagt tgccgagcca
     4501 attgggcatc agcgcggcac cccgccggcg cgccgtgaac gtcgccggcg cacaggcttg
     4561 cccgcggacc gcctggcagc gctctcagcc ggcggggtgg gtcctcgggc acgcgccgcg
     4621 ccggcggcga gccaagccga ttgcgtgccg cagagcgcag gagggggtga ggctcggcgc
     4681 cgccagaacg cccatcatcg ccgcccccgg ccaagccggg gaaacatttg ggggcacggc
     4741 cctcaatcga tagctttcca ctctctgagc gcggcccaaa ggatcttaat ttctttttcc
     4801 gccgcaatat tgcgaaatga aggaatatca ttctatttaa cggcaaggcg ccggtccccc
     4861 tcctcgagcg cccgcaaaaa acctggggga ggcgccgcgc aggcggccgc gtccttctga
     4921 gccgtgggca gcagagcatg ggcagcattc gcgtgaacga ccacaacggc aggacgcacg
     4981 tcctggaggc ggtggagggc tggcgggtga tggagctcat tcgcgaccac ggtctgccga
     5041 tcgaagcgat ttgcggcggg gcctgcgcct gcgccacctg ccacgtgcat gtcgccccgg
     5101 cctgggccga gcgcctctcg cccatgcgcg aggacgagga aggtctgctc gatgcgctgc
     5161 cgcaggcggc cgacacctcg cgcctgtcct gccagatcat ttgggacagc gatcttgatg
     5221 ggctcgaact caccctggtg ggggatggcg catgaaatac tttcccctgt tcacccgtct
     5281 cgacggggcc gaggttctcg tcgccggcgg cggcgaagcc gcactgcaaa aggtccgcct
     5341 tctcctgaag acgacggcgc acatcacgct gatgacggca agcctgcacc cggagttgcg
     5401 cgcgcttgtc gatgccggcg cgctgaccca cgtgccccgc ctgcccggcc cggccgacct
     5461 cgagcggcaa agcctcgtct atgccgccac cggcgatcgc acc
//
LOCUS       DASTMT010000569         1976 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_3779603, whole genome shotgun sequence.
ACCESSION   DASTMT010000569 DASTMT010000000
VERSION     DASTMT010000569.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 1976)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1976)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1976
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3779603"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            385..>1976
                     /locus_tag="VFR00_13015"
     CDS             385..>1976
                     /locus_tag="VFR00_13015"
                     /inference="COORDINATES: protein motif:HMM:NF025214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU0160235.1"
                     /translation="MSKPTSTTTPPAGISKAEAIRRAMALHRGGKLEEAARLYRAVLS
                     AAPNDFDAMHLLGVLLSSRRQSAEGIALIKRALALQPNFAGAHNNLGTALLAAKRPEE
                     ALASFRRAVELKGDFAEAHSNLGNALADLGQREEAVACHRQAIALKPDYAEAYNNLGN
                     VLIALGRFAEAAEACQHALTLKANYAKAHNNLGTARARQRREEEAIAAFRQAIAANTD
                     FAEAHRGLGEALLALRRDDEAVVALERAIKLRPDDADAHIGLGSALSAQRQMGGAANA
                     YRRAIALQPDNAVAHHNLGNALAAAGELDAAAASYQRASEAMTDDAHALLQYVQVSKQ
                     MCAWRQGAAPAAALLARARHGNVNGPPFPLLAVADDPAVHYAAARTFVGHSIGRDYPV
                     LWNGEGYRHKRIKLAYFSFDLREHPAARLIVDLMERHDRSQFEVYAVSSGPDDKSALR
                     RRVSQACDHFIDVRPLADAAVAQKLRALEIDILVDLDGHTEGSRSRALAWHPAAAQVA
                     YLGYPATMGADFIDYALVDRTVV"
BASE COUNT          350 a          683 c          641 g          302 t
ORIGIN      
        1 gcagcggcga aaaggtgttg gtctcggtgg caagcgtcgc cgtgaagatg cgcattttgc
       61 tgtctctcag cctggacggg gtgcccgcca agagaggcga gccctccttg ttggggcaag
      121 gcaacgccaa gcgtcaaggc cagcagcacc tgccgggccc gaagctgtgg catcgccaga
      181 cccgcgctgg ccacggcaca agcgccggcc tgggcagccg aggctgacgt gcgtgggcaa
      241 acgctcgccg gcggactggg cggacgcccg tagccaagag gcgccggagg tcgtggcgct
      301 ttttcgcatt ttgctcgccg ctgttgcgct gcggctgata gaacggcggg cgaggcggca
      361 gccgccgcaa gccatggacc acgcgtgagc aagcccactt cgaccaccac cccgccagcc
      421 ggcatcagca aggccgaggc cattcgtcgg gcaatggccc tgcatcgcgg cggcaagctc
      481 gaggaggcgg cgcgccttta tcgtgccgtc ctcagcgccg cacccaacga ctttgatgcc
      541 atgcacctgc taggcgtact gttgagctcg cggcggcagt cggcggaggg catcgcgctg
      601 atcaaacggg cgctggcgct gcagccgaac ttcgccggcg cacacaacaa tttgggcacg
      661 gcgctgctcg cggcaaaacg gcccgaagaa gcgctcgcaa gctttcggcg cgccgtcgaa
      721 ctcaagggcg atttcgccga ggcgcacagc aacctcggca atgcgctcgc cgaccttggc
      781 cagcgcgagg aggccgtcgc ctgccatcga caggccattg ccctgaaacc ggactacgcg
      841 gaagcctaca acaatctggg caacgtcctc atcgcgctcg ggcgctttgc ggaggcggcc
      901 gaggcatgcc aacacgccct cacgctcaaa gccaattatg ccaaggcgca taacaatttg
      961 ggcacggcgc gtgcgcgaca acggcgcgag gaagaggcga tcgccgcctt tcgccaggcc
     1021 attgccgcca acaccgattt cgccgaggcc catcgcggcc tgggcgaagc gttgttggcg
     1081 ctgcgacgcg acgatgaagc cgtcgtggcg ctcgagcgcg ccatcaagtt gaggcccgat
     1141 gacgccgatg cccacatcgg ccttggcagc gccttgagtg cgcagcggca aatgggcggt
     1201 gcggccaacg cctatcggcg cgccatcgcc ttacaacccg acaacgcggt cgcccaccac
     1261 aacctcggca acgccttggc cgccgccggc gagctggatg ccgctgccgc cagctaccag
     1321 cgtgccagcg aagccatgac cgacgatgcc cacgcgctgt tgcagtatgt gcaggtatcg
     1381 aagcaaatgt gcgcctggcg gcaaggggca gcgccagcgg cggcgctgct ggcacgcgcc
     1441 cggcatggca acgtcaatgg tccgcccttt cccctcctcg ccgtcgccga tgatcccgcc
     1501 gtgcactacg cggcggcgcg aaccttcgtg gggcacagca tcggccgcga ctatcccgtg
     1561 ctgtggaatg gcgagggcta ccggcataag cgcatcaaat tggcctattt ctccttcgac
     1621 ctgcgcgaac atcccgccgc acgtctgatc gtcgatctga tggaacgcca cgaccgttcc
     1681 cagttcgagg tgtacgcggt ctcgtccggc cccgacgata agagtgcgct caggcgtcga
     1741 gtgtcgcagg cctgtgatca cttcatcgac gtgcgaccgc ttgccgatgc cgccgttgcg
     1801 caaaaactgc gcgcactcga gatcgacatc ctggtcgatc tcgacggcca cacagaggga
     1861 agccgcagcc gggccctggc ctggcacccg gcagccgcgc aggtggccta cctcggctac
     1921 ccggccacca tgggtgccga tttcatcgac tatgctctcg tcgaccgcac cgtggt
//
LOCUS       DASTMT010000570         2556 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_5428334, whole genome shotgun sequence.
ACCESSION   DASTMT010000570 DASTMT010000000
VERSION     DASTMT010000570.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2556)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2556)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2556
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5428334"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            337..1980
                     /gene="ligD"
                     /locus_tag="VFR00_13020"
     CDS             337..1980
                     /gene="ligD"
                     /locus_tag="VFR00_13020"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016736649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HEU0160236.1"
                     /translation="MAKKALALYQAKRDFKKTPEPRGAAAPGHRQYPRFVVQKHAASR
                     LHYDLRLEVAGVFKSWAVTKGPSLDPADKRLAVEVEDHPLDYGDFEGTIPKGEYGGGT
                     VMLWDRGFWAPEGNADPARALRAGELKFSLAGKKLKGGWVLVRMRRKGERSNVNNWLL
                     IKHRDGFERAGDGALLAQDRSVASGRSMQQIAEGKGRGPRPFMELGEPRADPRAQWRA
                     ETTANGTGKPEPRRSRAQAQDSETEGPGVLGVKLSKPDKELWPATEESKPFSKLDLAR
                     YLETVGPWMLEHIGGRPCSILRAPDGINGERFFQRHAMPGLSKLITLTPVGRHVKPYL
                     QIDRFEALIALAQVATVEFHPWNSAPGRPERPGRLIFDLDPGSDVAFALVIAAAKELR
                     ERLEQLGLVAFVKTTGGKGLHVVTPLTLKGNGPSWLEAKRFAGAVCAEMAHDQPERYL
                     VNMRKALRGGRIYLDYLRNDQTSTAVAPLSPRARPGAPVSMPLTWSQVRDGLDPGRFT
                     LASAAGLIAKSSAWQEYRQAARPLAAAIRKLRPGKPIAR"
     gene            2040..>2556
                     /locus_tag="VFR00_13025"
     CDS             2040..>2556
                     /locus_tag="VFR00_13025"
                     /inference="COORDINATES: protein motif:HMM:NF013252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HEU0160237.1"
                     /translation="MESRAVAAVPTGPGWQYEPKWDGFRCLAFRAGGEVEIKAKSGKS
                     LARFFPEVLANLRALKQRAFVLDGELTIARDGALSFDALQARLHPAESRIRRLAAEAP
                     ATFVAFDCLLVKRGQPLLQAPLLQRRAVLEEAFGGSRAETHGLALTPYTRACAEAQTW
                     LQGTESSFDGVI"
BASE COUNT          418 a          827 c          890 g          421 t
ORIGIN      
        1 acaaccgggg ccttgcgcgc gggcgccaga cctagcattg ccgccgccgc cgggcaacga
       61 atatccgcgc gccggcgccg gcgctcaacg ggggtcgcct gcaaaaccct cctctcagct
      121 gagatgtccg attgagattg gctgcgctgc gctcgggttt gagcctggct tgggcggggt
      181 cctcggaggt cggatggcag cccggcctgg ttcgaggtca acgtggtctt gccgctgcgg
      241 ccaggcgcgg tggggtgctt aaggccgcgg gaacggcccc accgcgttgg ccgttgagct
      301 ggcgcgcacc ttgtgcgaac aaccccggag gtcccgatgg cgaagaaggc tctggccctt
      361 taccaagcca aacgcgactt caaaaaaacg ccggagccgc gcggtgcggc cgcgcccggc
      421 caccgccaat acccgcgctt tgtcgtgcaa aagcacgctg ccagccgcct gcactacgat
      481 ttgcgcctgg aggtggcggg cgtcttcaaa tcgtgggcgg tcaccaaggg tccctcgctc
      541 gatcccgccg acaagcgctt ggcggtcgaa gtggaggacc atcccctcga ttatggagac
      601 tttgagggca ccatccccaa gggagaatat ggcggtggca ccgtcatgct atgggaccgg
      661 ggtttttggg cgccggaagg caacgccgac ccagcccggg cgctgcgcgc gggggaactg
      721 aagttttccc tggcgggcaa aaaactgaag ggcggctggg ttcttgttcg catgcggcgc
      781 aagggcgagc gcagcaacgt caacaattgg ctgttgatca agcatcgcga cggttttgag
      841 cgcgcgggcg acggtgcgct gctggcgcaa gaccgctccg tggcttccgg ccgcagcatg
      901 cagcagatcg ccgagggcaa ggggcgcggt ccgcggccct tcatggagct cggcgaacca
      961 cgcgccgacc cgcgcgcgca atggcgcgcc gagaccaccg ccaatgggac cggcaagcca
     1021 gagccgcggc gctctcgcgc gcaggcgcag gacagcgaga cagaagggcc aggggtgctg
     1081 ggcgtcaagc tttccaagcc cgacaaggag ctgtggccgg cgacggagga gagcaagccc
     1141 ttcagcaagc tcgatctggc acgctatctc gaaacggtgg ggccgtggat gctggagcac
     1201 atcggtggcc ggccgtgctc gatcctgcgg gcgcccgacg gcatcaacgg cgagcgcttt
     1261 tttcagcgcc atgcgatgcc cggtctctcg aagctgatca ccctgacccc ggttggccgc
     1321 cacgttaagc cctacctgca gattgatcgg ttcgaggccc tgattgcgtt ggcccaggtt
     1381 gccaccgtcg aattccatcc ctggaactcg gcgccgggtc ggccggaaag gccggggcgg
     1441 cttattttcg acctcgaccc cggcagcgat gtcgcttttg cgttggtgat tgccgcggcc
     1501 aaggagctcc gcgaacggct tgaacaactt ggcctcgtcg cctttgtcaa gacgacgggg
     1561 ggcaagggtc tgcatgtggt cacgccgctc accctcaagg gcaatggccc aagctggctg
     1621 gaggccaagc gctttgccgg tgccgtctgc gccgagatgg cgcacgacca gcccgagcgt
     1681 tatctcgtca atatgcgcaa ggcgctgcgg ggaggtcgca tttatctgga ttatctgcgc
     1741 aatgatcaga cctccacggc ggtggcgccc ttatcgccgc gggcgcggcc gggggcgccc
     1801 gtatccatgc cgctgacctg gagccaagtg cgcgacggtc tcgaccccgg acgttttacg
     1861 ctcgccagcg cagccggcct gatcgccaag tcgtcggcct ggcaggagta taggcaagcg
     1921 gcgcgcccgc tcgcggccgc catccgcaaa ttgcggccgg gcaagcccat cgcccgctga
     1981 tgcgcaagcc gacgacgtcg ccgttatcga gcctcggcat tgagctgacg ttgcggccca
     2041 tggaatcccg agccgtggct gcggtgccga cggggcccgg ctggcaatac gaaccgaaat
     2101 gggacgggtt ccgatgcctg gcctttcgcg ccggtggcga ggtcgagatc aaggccaagt
     2161 cgggcaagtc gctcgcgcgg ttttttcccg aggtgctggc gaatttgcgc gcgctgaaac
     2221 agcgcgcctt cgtcctcgac ggggaactga cgatcgcgcg cgacggggcg ttgtccttcg
     2281 acgccttgca ggcacgcctg cacccggccg aaagcaggat ccgccgcctg gcggcggagg
     2341 cgccggcgac cttcgtggcc ttcgattgcc tgctcgtcaa gcgcggccag ccgctgctgc
     2401 aggcgccgtt gctgcagcgc cgcgcggtct tggaagaggc gttcgggggt agccgtgccg
     2461 agacacacgg gttggcgctc acgccctata cgcgcgcctg cgctgaggcg caaacatggt
     2521 tgcaggggac agaaagttcc ttcgacggtg tcatcg
//
LOCUS       DASTMT010000571         6202 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_4771855, whole genome shotgun sequence.
ACCESSION   DASTMT010000571 DASTMT010000000
VERSION     DASTMT010000571.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 6202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6202
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4771855"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..338
                     /gene="bcsS"
                     /locus_tag="VFR00_13030"
     CDS             <1..338
                     /gene="bcsS"
                     /locus_tag="VFR00_13030"
                     /inference="COORDINATES: protein motif:HMM:NF028346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsS"
                     /protein_id="HEU0160238.1"
                     /translation="IETEKELPYYFALEGNYSTAFNSYWARARVGWHRGRFIFGPEAS
                     VVGDEDFDAQRVGGFVRFDLPLAPFRPIEVTLSAGHQFVSGNNNNGATGGIGGSEGTY
                     GLINWSVTF"
     gene            536..3919
                     /locus_tag="VFR00_13035"
     CDS             536..3919
                     /locus_tag="VFR00_13035"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU0160239.1"
                     /translation="MTKRSEPATVRPIFFDSTGRRRLVVSALSWGGGSLLGILTACLI
                     VTSLFGPVLPSVALLGTPRELSSQRLTAPTSTGEPEYGGYRARSLAADAAARAARYAY
                     LVNWDDNSFSSLKRNARELDNLIVEWLHVGGPDGGLQHDAPAKEALVNDWLKTNAPQL
                     KVLPLVNNYDEARSRWAPEAAEAMLLSGSGRARFASEIEHFVSDGGYAGIVLDLEELP
                     ARAQSDYVKLVRELAGRFAGRGLQVLVALPAESSEYDTAALAAAADALILMSYDEHTE
                     KGLPGPLASQGWFETVLARHLKTIPADKLIVSIGSYGYDWQSGKPAQEISVQEAWELL
                     EESRAGLSFDKKALNPTFSYLDDGSKTQHQVWYLDAVTAYDQVAVALPAQPKGLALWR
                     LGTEDPSVWSLLGRGRSADANALAAVKTLHAGYDVLYKGRGEVLSVSGALQQGSREIL
                     YDPASNLITTQQILAFPKATTITRWGARNDKVLALTFDDGPDPRFTPKILDILAQKGA
                     KATFFIVGSAGALHPDLLQRIYREGHEIGNHTFTHVNTAIASPERLQFEINATQRLIE
                     STIGARTTLFRPPYGDDLEPQTIDGAQVLALAGSLGYLTIGMDIDPKDWRRPLASQIV
                     AATVEQAMKGDGKVILLHDAGGMRDATIEALPQIIDRLRVAGFRFLPVHELLGLSRDE
                     VMPRVGPEDAWITASNYAGFAFFKNMNSVLTLFFYAGIALGTVRLLWVAGSALVHARR
                     ERKRLHLAWTPTSIAALIPAFNEEKVICNSIRALLASPEANFEIIVIDDGSSDATAEV
                     VRRTFAHSERVRVLTKENGGKWAALNYGLAHTDAEVVVTLDADTQFEPDALRWLVRHF
                     ADPKVAAVAGAAVVGNQINLITRFQALEYVTNQNLDRRALELVNGITVVPGAIGAWRR
                     GALAAVGGFLPDTLAEDSEATVRLARRNWKVLYEPRAAARTEAPETVRAFMKQRLRWM
                     FGTLQVAVKNLSAIGRARPIGLSLFGLPNIIVFQFVFTLIAPAMDLMLLWSLISGLNN
                     YVSGPPDAVFPPALLHVAAYWAYFQTLEIATAALAIGIEQKCRGMWRLLPLLVVQRFC
                     YRQLLYVTALRATAAALKGRMLGWNKLIRSGRVVSQFGG"
     gene            3995..4717
                     /locus_tag="VFR00_13040"
     CDS             3995..4717
                     /locus_tag="VFR00_13040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0160240.1"
                     /translation="MAATARPRRPLFVGLVLSVVGGALLAMLAGAPAQPGAAAERTSP
                     MPLRKKEPQWKSALATVFWVGETETEDNDHIANLKSAWDAKWVEHFGGVDDPHSRCGY
                     EPCGFKPKENPFYVALPYDDMEEDGRRKAVNSFIPWDTPGAKQSLLKNRWVAIQANGI
                     TCYAQWQDVGPFEKDDADYVFGASATPKNSKGESAGIDLSPAVRDCLRVGGVSRVMWR
                     HVDWQEVPGGPWKHTITHRPGP"
     gene            complement(4841..5923)
                     /locus_tag="VFR00_13045"
     CDS             complement(4841..5923)
                     /locus_tag="VFR00_13045"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEU0160241.1"
                     /translation="MKFGWMTLAHSPSAADDAGAIFEQLEQATLAEHLGFDGVWLTEH
                     NFTGESVYADPIPFAAALAMRTQRIRIGFAVIQMALRHPVRLATQLSLLDNLCKGRLD
                     IGVGRGSIFNEYEFVGYGLRSDDARERMAEALEVMTRAWTATPLDFKGKYFAVRLPEL
                     RPAPVQRPHPPIWHSVASAASFHLCGKKGVPILTVRLPIAHIRERLGQYAEGLSASGL
                     AAATQQQLMRQAAIWRWVYVAESRAAAEDELAAALQATRRHMMHARQALNPADFHADP
                     ALMNPWTDPAHAEAEGIAWALRSGTLIGSAEDVAEQVAELRDAGVHHLLCQMSFGYLD
                     HARITRSMRRFAQRVVPAFRSGAPPR"
     gene            complement(5920..>6202)
                     /locus_tag="VFR00_13050"
     CDS             complement(5920..>6202)
                     /locus_tag="VFR00_13050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010914742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEU0160242.1"
                     /translation="LGGYGGLAFVGTPQSIADGMHEWLLTAGCDGFNVMFPYLPEGLD
                     DFVGRVVPELQRRGIFRTRYEGTTLRENLGLPRPANRNFAKAQAAGAAL"
BASE COUNT         1071 a         2012 c         2019 g         1100 t
ORIGIN      
        1 gcatcgagac cgaaaaggaa ttgccctact atttcgcctt ggaaggcaac tattcgaccg
       61 ccttcaacag ctactgggcc cgcgcgcggg tgggctggca ccggggtcgc ttcatctttg
      121 ggccggaagc cagcgtcgtt ggcgatgagg atttcgatgc ccagcgcgtc ggcggcttcg
      181 tcaggttcga tctacccttg gcgcccttcc gcccgattga ggtgacgctg tcggccggtc
      241 accagttcgt ctccggcaac aacaacaacg gcgccaccgg tggcatcggc ggcagcgagg
      301 gtacctatgg tctgatcaat tggagcgtga ctttctagcc aacctcgcca tttccactcc
      361 ccacaaggtg gacgaaaggc ctgcgacatt tcgtacgtaa ttgttaggca cctaagtccc
      421 tactggaaca cttctccgcg cattatctag cgtttctcgt cggttatcgc cgaaggctaa
      481 gacagctttc cggccgtgac cgtggtgcgg atgccttgcg gggtggggtt ctttagtgac
      541 caagcgtagc gagccggcaa ctgtccggcc gatttttttt gactcgacgg gccggcgtcg
      601 cctggtcgtt tcggccctga gttggggtgg cgggagcctg ctcggcattc tgaccgcctg
      661 cctgatcgtc accagcctgt tcggccccgt gctgccgagc gtcgccctgc tggggacgcc
      721 gcgcgaactc tcctctcaac gcctgacggc gccgaccagc accggcgagc ccgagtatgg
      781 gggatatcgc gcccgcagtt tggcggctga tgcggcggcg cgcgccgccc gctacgccta
      841 tctcgtcaac tgggacgaca acagcttctc ctcgctgaag cggaacgcgc gcgagctcga
      901 taatctgatc gtggaatggc tgcacgtcgg cggccccgat ggcggcctgc agcatgatgc
      961 cccggccaaa gaagccctgg tgaacgattg gctcaaaacc aatgccccgc agctcaaggt
     1021 gctgcccctg gtcaacaact atgatgaggc ccggtcccgg tgggcgcccg aagcggcgga
     1081 agcgatgctg ctgtccggct cgggccgggc gcggttcgcc tccgaaatcg agcactttgt
     1141 gagcgacggc ggctacgccg gcattgtcct cgatctggaa gaacttcctg cccgcgcgca
     1201 atccgactac gtcaagctgg tgcgcgagct cgccgggcgc ttcgccggcc ggggcctgca
     1261 ggtgctggtg gctttgcccg ccgagagcag tgagtacgat acggccgccc tcgccgccgc
     1321 ggccgatgct ctgatcttga tgagctacga tgagcacacc gaaaaaggcc tgcccgggcc
     1381 gttggccagc cagggttggt tcgaaaccgt cctggcccgc catctcaaaa ccattcctgc
     1441 cgacaagctg atcgtcagca tcggctccta cggctacgac tggcagagtg gcaaacctgc
     1501 ccaggaaatc tccgtgcagg aggcttggga gctgctggag gagtctcggg ccgggttgag
     1561 ctttgacaag aaagcgttga acccgacctt cagctacctc gacgatggca gcaaaaccca
     1621 gcaccaggtc tggtacctcg atgcggtgac cgcctacgat caggtcgccg tggcgctgcc
     1681 ggcccaaccc aaggggttgg ccttgtggcg gctgggaacc gaggatccaa gcgtatggtc
     1741 gctgctcggc cgaggccgct cggcggacgc caacgcgctg gccgccgtca agaccctgca
     1801 tgccggctac gacgtgctct acaagggccg gggcgaggtc ttaagcgtca gcggcgcgct
     1861 gcagcaaggc tcgcgcgaaa tcctctacga tccggccagc aacctcatta ccacgcaaca
     1921 gatcctcgcc ttccccaaag caaccaccat cacgcgctgg ggcgcgcgca acgacaaggt
     1981 gcttgccctg acgttcgatg acgggcccga cccccgcttc acgcccaaga tcctcgacat
     2041 cctggcgcag aaaggcgcga aggcgacctt ctttatcgtc ggttccgccg gagccctgca
     2101 tcccgatctc ttgcaacgca tctaccgtga ggggcacgag atcggcaatc acaccttcac
     2161 ccatgtcaat acggccattg cctcgcccga gcgcttgcaa ttcgagatca acgccacgca
     2221 gcggctgatc gagtcgacga tcggcgcgcg caccacgctg tttcgtccgc cctacggtga
     2281 cgatctcgag ccgcaaacga tcgacggggc gcaagtcctg gcccttgctg gctcgctcgg
     2341 ttatctcacc atcggcatgg atatcgaccc caaggattgg cgtcggccgc tcgcctccca
     2401 gatcgttgct gccaccgtgg agcaagccat gaagggcgat ggcaaagtca tccttctgca
     2461 cgatgccggc ggcatgcgcg acgcaaccat cgaggcgctg ccgcagatca tcgacaggct
     2521 gcgcgtcgcc ggcttccgtt tcttgccggt gcatgagctc ctcggcttgt ctcgcgacga
     2581 ggtgatgccg cgtgttggcc ccgaggatgc ctggatcacc gcctcgaatt atgcgggctt
     2641 tgcctttttc aagaacatga attccgtcct gacgctgttc ttttatgcgg ggatcgcact
     2701 tggcacggtg cggctgttgt gggttgcggg ttcggccctc gtgcacgcgc gccgcgagcg
     2761 caagcggctg cacctggcgt ggacgccgac ctcgattgcc gcgctcattc ccgccttcaa
     2821 tgaagagaag gtgatctgca actcgatcag ggcgctgctt gcctccccgg aggccaattt
     2881 cgaaatcata gttatcgacg acggatccag cgatgccacg gccgaggtcg tacgccggac
     2941 atttgcccac agcgagcgcg tgcgcgtgct cacgaaggaa aacggcggca agtgggcagc
     3001 gctcaactat gggctggccc acacggatgc cgaggtcgtg gtcacgctcg atgccgacac
     3061 acagtttgaa cccgatgcgc tgcgttggct ggtccggcat tttgccgacc ccaaggtcgc
     3121 cgccgtcgcc ggcgcggccg ttgtcggcaa ccagatcaat ctcattactc gcttccaggc
     3181 cctggagtat gtaacgaacc agaacctcga ccggcgcgcg ctcgagctgg tcaacggcat
     3241 cacggtcgtg cccggcgcca ttggcgcctg gcggcgcggc gcgctcgcgg ccgtcggagg
     3301 tttcctgccc gatacgctcg ccgaggactc cgaggcaacg gtgcgcttgg cgcggcgcaa
     3361 ttggaaggtg ctctacgagc cgcgggcagc cgcgcgtacc gaggcaccgg agaccgtcag
     3421 ggccttcatg aagcaacgcc tgcgttggat gttcggcacg ctgcaggtgg cggtcaagaa
     3481 tttatcggcc atagggcggg cgcggccgat cgggctcagc cttttcggtc tgcccaacat
     3541 catcgtgttt cagttcgttt tcacgctcat cgcgccggca atggacctga tgctgctgtg
     3601 gagcttgatc tccggcctca ataattatgt gtcgggtccg cctgatgcgg tgtttccccc
     3661 ggcgctgctg cacgtggccg cctactgggc ctacttccag accctggaaa tcgccaccgc
     3721 cgcgctggcg attggcatcg agcagaaatg ccggggcatg tggcggctgt tgccgctcct
     3781 ggtcgtgcaa cgcttctgtt atcggcagct cctttacgtc acggcgctgc gggcgacggc
     3841 ggcagcgctg aaggggcgaa tgctcggttg gaacaagctc atccgcagcg gccgcgtggt
     3901 cagccaattc ggcggctgaa tcacgcacat gtgtcggcac gaactggcgt ttctcgttat
     3961 gtttccaaca tccagggagt gaagatcgcg caatatggct gcaacggcac ggccccgccg
     4021 cccactgttc gtgggcctcg ttctcagcgt cgtgggtggg gcgctgctgg caatgcttgc
     4081 cggtgcgccg gcccaacccg gtgcggcggc ggaaaggacc agccccatgc ccctgcgcaa
     4141 gaaagagcca cagtggaaaa gcgcgctcgc cacggtgttc tgggttggcg aaaccgagac
     4201 cgaggacaac gatcacattg ccaacttgaa gagcgcttgg gatgcgaagt gggtcgaaca
     4261 tttcggtggc gtcgatgatc cccacagccg ctgcggctac gagccgtgcg gcttcaagcc
     4321 aaaggaaaac ccgttttacg tggcgctgcc ctacgatgac atggaggagg acggtcgccg
     4381 caaagcggtg aactcgttca tcccttggga cacgccaggc gccaaacagt ccctcctgaa
     4441 aaaccgctgg gtggccattc aggccaacgg tatcacctgc tacgcgcaat ggcaggacgt
     4501 cggcccgttc gagaaagacg atgccgatta cgtcttcgga gcctcggcga cgccgaagaa
     4561 cagcaaaggc gaaagcgccg gcatcgactt gtctcccgcg gtcagggatt gcctcagggt
     4621 tggcggcgtc tcgagggtga tgtggcgcca tgtcgattgg caagaggttc cgggcgggcc
     4681 ctggaagcac acgatcacgc accggcccgg gccctagccg cacgccgggc acgccgggct
     4741 gcccgctcgc tgcggcctca cgcgcgctgg cagcgggccg cggctggcgc agcggcggtg
     4801 cggtgaggac cggggcgccc gcccggctcg cgagcgtgcc tcagcgcggc ggcgcgccgg
     4861 accgaaaagc cggcactacc cgctgcgcaa accggcgcat ggagcgcgtg atgcgcgcgt
     4921 ggtccaggta gccgaagctc atctggcaga gaagatggtg gacgccggca tcgcgcagct
     4981 cggcgacctg ctcggccacg tcctcggcgc tgccgatcag ggtgccggag cggagcgccc
     5041 aggcaattcc ctccgcctcg gcatgggcgg gatcggtcca ggggttcatg agcgcgggat
     5101 cggcatggaa atcggcgggg ttcaaggcct ggcgcgcgtg catcatatgg cgccgcgtcg
     5161 cctgcaaggc ggcggcaagc tcgtcctcgg cggcggctcg gctctccgca acataaaccc
     5221 aacgccagat cgccgcctgc cgcatgagct gctgctgcgt ggcagcagca agcccgctgg
     5281 cgctgagccc ttcggcatat tggccgagcc gctccctgat gtgcgcgatc ggcagccgca
     5341 cggtcaggat cgggacgccc tttttgccgc acagatgaaa cgatgccgcc gaggcaacgc
     5401 tatgccagat cggtggatgc ggccgctgca ccggggcggg gcgcagctcc ggcagacgca
     5461 ccgcgaaata tttgcctttg aagtcgaggg gcgtcgcggt ccaggcgcgc gtcatcacct
     5521 ccaaagcctc ggccatgcgc tcgcgtgcat catcgctcct gagcccatag ccgacgaatt
     5581 cgtattcgtt gaagatgctg ccgcgcccga cgccgatatc aagccgcccc ttgcacaggt
     5641 tatcgagcag cgagagctgc gtggccagcc tgacgggatg gcgcaaggcc atctggatga
     5701 cggcgaaacc aatgcggatg cgctgggtgc gcatcgccaa cgcggccgca aaggggatcg
     5761 ggtcggcata gacgctctcc ccggtgaagt tgtgttccgt cagccagacg ccgtcgaagc
     5821 ccaagtgctc ggccagggtc gcctgctcga gctgctcgaa gatggcgccg gcgtcgtcgg
     5881 cggcggaggg ggagtgcgcc agcgtcatcc agccgaactt catagggctg ctcccgcggc
     5941 ttgagctttg gcaaagtttc ggttggccgg ccgcggcagg ccgaggttct cgcgcaaggt
     6001 tgtcccctcg tagcgggtgc ggaagatccc gcgccgctgc aactcgggga cgacgcggcc
     6061 aacgaagtca tcgaggcctt cgggtaggta ggggaacatc acgttgaaac cgtcacagcc
     6121 cgcggtcagc agccactcgt gcatgccatc ggcgatgctc tgcggcgtgc cgacgaaggc
     6181 aagcccgccg tagccgccga gg
//
LOCUS       DASTMT010000572         4081 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_4777114, whole genome shotgun sequence.
ACCESSION   DASTMT010000572 DASTMT010000000
VERSION     DASTMT010000572.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4081
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4777114"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..462)
                     /locus_tag="VFR00_13055"
     CDS             complement(<1..462)
                     /locus_tag="VFR00_13055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016848223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HEU0160243.1"
                     /translation="MLPDTDPITVSVVQHRLRAIVEEMGEAMLRTSYSQILNSSRDFS
                     TALCDPEGRLVAQAEHVPIHVGALPFAAQAVHDFFKGRIRPGDVYLLNDPYCGGNHLP
                     DLTVFVPVFDQGRLVFWSINRSHQSDIGGATHGAYNAGATEIYQEGLRIPPL"
     gene            complement(564..1883)
                     /locus_tag="VFR00_13060"
     CDS             complement(564..1883)
                     /locus_tag="VFR00_13060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018099655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEU0160244.1"
                     /translation="MSPNPPRNSDLDSALAEARERYRSCRPESERIHRAARAVLPGGN
                     TRSVLFYTPFPTAMTRGVGCFLEDVDGQRYLDFCGEYTAGLFGHSEPRIQAALHAAID
                     RGLNLAAVGERESQLAALLCARFPSLQRVRFTNSGTEANMMALGAARAFSGRPGILAF
                     RGGYHGGLLTLVGGGSPINAPIPVTLADYNDTQQTLEVLRTHAADIGAVILEPMMGSG
                     GCIPARPDFLAALRQASRELGILLLFDEVMTSRHSAGGLQKLHGVTPDLTTLGKYMAG
                     GMSFGAFGGRADVMGLFDGHRPGALIHSGTFNNNVMTMAGGMVAMGEIFDAAAADQLF
                     ARGEDLRAKLNAICRARAVEMHFSGLGSMMHPHFRRGPIERPYTPTAREDALRELYFF
                     DLLAQGIYIARRGMIAMSLPIGAAECESLLAAVEEFCTLRAPFLTGS"
     gene            2316..2960
                     /locus_tag="VFR00_13065"
     CDS             2316..2960
                     /locus_tag="VFR00_13065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007592082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 domain-containing protein"
                     /protein_id="HEU0160245.1"
                     /translation="MLDWFQRLLPREEKFFPLFARHAGCIAAAAQALRGMLDGGEAVL
                     AHCAAVMRHEEDADAITREVLIGVRSTFITPFDRGDIKDLITAMDDAIDQMQKTAKVI
                     VLFEMSAFAPQMRSIADHIGHCSELVRRAMPLLANIGQHAGQLTEICQQISRIEGQAD
                     DLYDQGLKQLYARVKGGDAMEFVRSNEIYDHLEKSVDRFDDIANQIQGIVIEHV"
     gene            2978..3979
                     /locus_tag="VFR00_13070"
     CDS             2978..3979
                     /locus_tag="VFR00_13070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004671834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HEU0160246.1"
                     /translation="MDGAGLGLLFGLVAVALIFDFLNGLHDAANSIATIVSTRVLPPR
                     AAVVWASFFNFIAFLFFGLHVAQTLGTGIIDAGVVTPRVIFAALTAAIVWNLVTWWAG
                     IPSSSSHALIGGLIGAGLASAGLSAIVWSGVLKTVAAIVLSPAVGFVLALALVLAVSW
                     TFLGQTPFAVDHLFRRLQFVSAALYSLGHGGNDAQKTMGIIAGLLFAQGHLGETFYVP
                     VWVVLSCQAAMALGTLFGGWRIVKTMGSKITRLTPMQGFCAEAGGALTLFAANWLGIP
                     VSTTHTITGAIVGVGAARRVSAVRWNVASDVVTAWVLTLPSAALIAALAYAAVTPFT"
BASE COUNT          683 a         1322 c         1354 g          722 t
ORIGIN      
        1 gaggggcgga atgcgcagtc cttcctggta gatttcggtg gcacccgcgt tataggcacc
       61 atgagtggcc ccgccgatgt cgctctggtg cgagcgattg atcgaccaga acaccagtcg
      121 cccctggtcg aaaaccggca cgaacacggt caggtcgggc agatggttgc cgccgcaata
      181 ggggtcgttc agcagataaa catcgccggg ccggatgcgt cccttgaaga agtcgtgcac
      241 ggcctgcgcc gcaaaaggca gcgcgccgac gtggatcggc acgtgctcgg cctgggcgac
      301 aagccgccct tccggatcgc aaagggccgt cgaaaaatca cggctcgagt tgaggatttg
      361 cgaataggac gtgcgcaaca tcgcctcgcc catctcctcg acgatggccc gcagccgatg
      421 ctggaccacg gagacggtga tcggatcagt gtcagggagc atgagatctc aggtcgtggt
      481 tccggcgggc aaggcccgcg agcatgcatt ggccgaccaa ccccggtcaa ctcggcaggg
      541 gcgctcgagc cgggcgcctg cgctcaagac cccgtcagga agggggcgcg cagggtgcag
      601 aattcctcga ccgcggcgag caggctttca cactcggcgg ccccgatcgg caggctcatg
      661 gcgatcatcc cgcggcgcgc gatatagatc ccctgggcga gcagatcgaa gaagtagagt
      721 tcgcgcaacg cgtcctcccg ggccgtgggg gtataggggc gttcaatcgg gccacgccgg
      781 aaatgggggt gcatcatgct gccgagcccg ctgaagtgca tctcgaccgc ccgcgcgcgg
      841 caaatggcat tcagcttcgc gcgcaggtcc tctccgcgcg cgaacagttg atcggcggcc
      901 gcggcatcga agatctcgcc catggcaacc attccgcccg ccatggtcat aacgttgttg
      961 ttgaaggtgc ccgagtggat cagggcgccg ggccgatggc catcgaaaag gcccatcaca
     1021 tcggcgcggc cgccaaaggc accgaagctc atgccgccgg ccatgtactt gccgagcgtg
     1081 gtgagatccg gtgtcacgcc gtgcagcttc tgcaagccgc ccgcggaatg cctgctggtc
     1141 atcacctcgt cgaacagcaa aaggatgccg agttcgcggc tggcctggcg cagcgcagcc
     1201 agaaagtcgg gccgcgccgg tatgcagccg cccgagccca tcatcggctc gagaatgacc
     1261 gcgccgatgt cagcggcgtg tgtgcgcaga acctcaagcg tctgttgggt gtcgttgtag
     1321 tcggccaagg tgacgggaat gggtgcattg atcgggctac cgcccccgac tagcgtcaac
     1381 aggccgccgt ggtatccgcc cctaaaggca aggatgcccg gccggccgga aaaggcgcgc
     1441 gcggccccga gcgccatcat gttggcctcg gtgccgctgt tggtgaagcg cacgcgctga
     1501 agcgagggga agcgcgcgca cagcaaggcg gccagctggg actcgcgctc tccgaccgcg
     1561 gccaagttga ggcctcgatc gatcgcggca tgcagagccg cctgaatgcg cggttcggaa
     1621 tggccgaata gcccggccgt gtattcgccg cagaagtcga ggtagcgctg cccgtccacg
     1681 tcctcgagaa agcaaccgac cccgcgcgtc attgccgtgg ggaacggcgt atagaacagc
     1741 accgaccgcg tgttgcctcc cggcagcacc gcccgcgccg cccggtggat gcgttcgctc
     1801 tccggccggc aggagcgata gcgctcccga gcctccgcga gcgcgctatc aagatcgctg
     1861 ttgcgcggtg ggttcgggct cattgggcgc gtttccttgt gcgttgtgcc gcatcgccgg
     1921 gagggcgaac ggtgccggcg gctcgggtac aacgccggcg gcctcggcat tgtgcacgag
     1981 cgccagccag agcgcgatag ccggcttggc ctgcccgctc gccccgggcc gtttgtcacg
     2041 gcaacgcgcg caaaaaaagg ctggcgggtt gccccatttt tgcgccgcgc cggccgcgcg
     2101 gcagcggcga cagccgcccc ggcagcaggc cgtgggcacc cccgccggca ggccgctggc
     2161 cctccggtgc gcgcaatgcc acgcgggtgg ccgcccctgg tgggcgcgtg tttgcccaaa
     2221 aagcagcgcc agcggatttt gcttggcatg aggaagaagt aagctcggcc ccaaggcgcg
     2281 taggcttgct ggcacggcaa acgggcaacg gtgcgatgct tgactggttt cagcggttgc
     2341 tgcctcggga ggaaaagttt ttccccctgt ttgcccggca cgcggggtgc atcgccgccg
     2401 ccgcccaagc cttgcgcggc atgctcgacg gcggggaagc ggtcttggcc cactgcgccg
     2461 cggtcatgcg ccacgaagag gacgccgatg ccatcacccg cgaggtgctg atcggggtgc
     2521 gcagcacctt cattaccccg ttcgatcgcg gcgacatcaa ggatctgatc accgccatgg
     2581 acgatgccat cgatcagatg caaaagacgg cgaaggtcat cgtgctgttc gaaatgagcg
     2641 cctttgcccc gcagatgcgc agcattgccg accacatcgg tcactgctcc gagctcgtgc
     2701 gccgggcgat gccgctgctc gccaacatcg gacagcatgc cgggcagttg accgagatct
     2761 gccagcaaat cagtcgcatc gagggacagg ccgatgacct ctacgatcag gggctcaagc
     2821 agctctatgc gcgcgtgaag ggcggcgatg ccatggaatt cgtgcgcagc aacgagatct
     2881 acgaccatct ggagaagagt gtcgaccgct tcgacgacat tgccaatcag atacagggta
     2941 ttgtcatcga gcacgtttag gttggcgagg atgaggcatg gatggcgccg gtttgggttt
     3001 actgttcggg ttggtcgccg tcgccctcat cttcgatttc ctcaacggtc tacatgatgc
     3061 ggccaactcc atcgccacca tcgtctcgac cagggtcctg ccgccacgtg ccgccgtcgt
     3121 ctgggcctcg tttttcaact tcatcgcctt cttgttcttc ggtctgcacg tggcgcaaac
     3181 actgggtacc ggcattatcg atgcgggcgt ggtgacgccg cgggtcatct ttgccgccct
     3241 gaccgccgcc atcgtctgga acctggtgac ctggtgggcc ggcataccat cgagcagctc
     3301 gcatgccctg atcggcggct tgatcggggc ggggttggcg agcgccgggc tcagcgccat
     3361 cgtctggtcg ggagtgctca agacggtggc tgccatcgtg ctgtcgccgg cggtaggttt
     3421 cgtcctcgcc ctcgccctcg tgttggccgt ttcctggacc tttcttggcc agacgccgtt
     3481 tgcggtcgac cacctcttcc gtcgccttca gttcgtctcc gccgccctct attcgttggg
     3541 ccatggcggc aacgatgcgc aaaaaaccat gggcatcatc gccggcttgc tgttcgctca
     3601 ggggcatctg ggcgagacct tctacgtgcc cgtatgggtg gtcctcagtt gccaggcggc
     3661 gatggcgctg ggcacgctgt tcggcggctg gcggatcgtc aagaccatgg gctcgaagat
     3721 cacccgcctc acgccgatgc agggattttg cgccgaggcc ggcggtgcgc tgacgctgtt
     3781 tgccgccaat tggctgggca ttcccgtgtc caccacccat accatcaccg gggccattgt
     3841 cggggtcggc gccgcccgac gggtatcggc agtccgctgg aacgtcgcca gcgacgtggt
     3901 cacggcctgg gtgctcacat tgccctccgc cgctttaatc gccgcgcttg cctatgcggc
     3961 cgtgacaccc tttacctgag cgtcgcccgc acgccacgga caatcgcgct gcgatcgggt
     4021 aagttgccgc ttcggcaatt ttcgagcgcc aacatgcagc ggcccaacgt caaggacttg
     4081 c
//
LOCUS       DASTMT010000573         4210 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_4784341, whole genome shotgun sequence.
ACCESSION   DASTMT010000573 DASTMT010000000
VERSION     DASTMT010000573.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4210)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4210
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4784341"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            116..1519
                     /locus_tag="VFR00_13075"
     CDS             116..1519
                     /locus_tag="VFR00_13075"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEU0160247.1"
                     /translation="MRLAGRSWAGTGVFVAWAAALAVVPEPCRAQTEIPKTPPPQRAL
                     LKERAPATPGPVPSAPEPKAESKPEGKGKARAEAWTAEVVGRGISVLSAEIAPEGRRT
                     RFSLLLSASVPYQVFTLPDPYRVIIDIPDVAFQLPTGAGQQGSGLIAAYRYGHFAPGK
                     SRIVIDATGPVRVESQSLLPHGANKGGVRLSLELAATDPQSFLTRVPPRPKEGASRRE
                     AAAPAVGRPKADAKPQIVIDAGHGGVDPGAISGEVVEKDVVLAVARHLQAILAAKARY
                     NIHLTRAADVYVSLDKRVGLSRDKACDLFISIHADTVGAGASDLAQAVRGATVYVLSE
                     KASSREAERLADKENASDVLAGVDGSAEEETDHVKGILIDLMRRETADFSADFRGRLL
                     GHLKRRIALSRDPGRSAAFKVLKQSQSPSVLIELGYMSNAEDAKLLVSRDWQRQVAGS
                     IAAAVDEYFLERGGERR"
     gene            1689..>4210
                     /locus_tag="VFR00_13080"
     CDS             1689..>4210
                     /locus_tag="VFR00_13080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1A"
                     /protein_id="HEU0160248.1"
                     /translation="MRPTPILPPPAAPKKKRKRRSWLLSLLGFGFASAVVMFLAAAGV
                     VGFFFWKAARDLPDYESLAKYEPPVMTRIHAHDGSLMAEYARERRIFVPINVIPKLVI
                     AAFLSAEDKRFYEHGGLDFAGIGRAVINNFQNWGKKRPEGASTITQQVAKNFLLSSEQ
                     KLDRKLKEAILAIRIERAYSKDKILELYLNEIYLGIGSYGVAAAALNYFSKELSELSI
                     EEAAYLAALPKAPNNYHPFRKTREATVRRNWIIDQMAEVGYINGEQARLAKAKPLTVN
                     IRPFGTQIYAADYFAEEVRRRLMEMVGEDGLYGRAERTAIADGTVNGGLSVRTTLDPK
                     LQKTARRALIDGLVAFDREKGWRGPVQRIEIAGDWGAALAAVEAPNDLAPWRLGVVLD
                     AQRGKAVVGLRPGRQADGSLAAEREAVEIPFEELKWARTNRGVPKVVSDVVNNGDVVW
                     VAPKDAGNAAGTWSLMQIPEVGGAIVAMDPHTGRVHAVVGGFSFATSQFDRAVQARRQ
                     PGSSFKPLVYAAAIDNGYKPTSIVLDAPIEIDQGPGKEVWQPKNYDGVNSYGPSTLRT
                     GIEKSRNQMTVRLAQDMGMPLIVEYSKRFGVYDDLLPVLAMSLGSGETTLLRMVAAYA
                     ELANGGKQVHATLIDRIQDRWGRTIWRHDNRECTGCKVDNWQGQPEPVLADDRKQILD
                     PHTAYQMTSIMEGVVQRGTATIIKKYIPNVPVAGKTGTSNEEKDAWFIGYTPDLVVGI
                     FVGYDTPRPMGKGMTGGHVAAPIFGDFMRMVLADKKAVPFRIPPGIKLVRVSLRTGMR
                     TQGGEADSVLEAFKPYEDPDDPYSIVGFTNESGGFFMGDQQDA"
BASE COUNT          753 a         1394 c         1376 g          687 t
ORIGIN      
        1 gaaagttttg ctaaccacca attgcgtcat caggccgttg aattagcctt cgattcggca
       61 aacaagttgt taaactccac gtccttggcg cgcgctgaca gggatgggga gggcgatgcg
      121 actggccggc cgcagctggg caggcactgg cgttttcgtc gcctgggcgg cggcgctcgc
      181 cgtcgtgccc gagccgtgcc gggcccagac cgagatcccc aaaaccccgc cgccgcagcg
      241 cgcgctcctg aaggagcgcg ccccggccac cccggggccc gtgcccagcg cgccggagcc
      301 gaaggccgaa agcaaaccgg agggcaaagg caaggcccgg gcggaggcct ggacggccga
      361 ggtggtgggc cgtggcatta gcgtgctcag cgccgaaatc gcgccggaag gccgccgcac
      421 acgcttttcg ttgttgctgt cggcgagcgt gccctaccag gtctttacct tgcccgaccc
      481 ctatcgcgtc atcatcgaca tccccgatgt ggcctttcaa ctgccgaccg gcgccggtca
      541 gcagggctcg ggcttgatcg cggcctaccg ctatggccac ttcgcgccgg gcaagtcgcg
      601 catcgtcatc gatgccaccg gcccggtgcg ggtcgagagc caaagtttgc tgccgcacgg
      661 agccaacaaa ggcggcgttc gcctcagcct cgagcttgcc gccacggacc cgcaatcctt
      721 tctcacccgc gtgccgccgc ggccgaagga gggggcctcg cggcgcgagg ccgccgcgcc
      781 ggcggtgggc cggcccaagg ccgacgccaa gccccagatc gtcatcgacg ccggccacgg
      841 cggcgtcgac cccggcgcga tcagcggcga ggtggtcgaa aaggacgtgg tgttggccgt
      901 tgcccggcat ctgcaggcga tcttggcggc caaggcgcgc tataacattc acctcacgcg
      961 cgccgccgac gtttatgtgt cgctggacaa gcgggtcggc ctgtcgcgcg acaaggcctg
     1021 tgatcttttc atttcgattc acgccgacac ggtcggggcc ggcgccagtg acttggcgca
     1081 ggccgtgcgc ggcgccacgg tctatgtgct ctccgagaag gcctcgagcc gcgaagccga
     1141 gcgcctcgcc gacaaggaaa acgcctccga tgtcttggcc ggggtcgatg ggtcggcgga
     1201 ggaggagacc gaccacgtca agggcatctt gatcgatctc atgcgccggg agacggccga
     1261 tttttccgcc gacttccgcg gccggttgct cggccacctc aagcggcgaa tcgcgctctc
     1321 ccgggatccg ggacgctcgg ccgcgttcaa ggtcttgaag caatcccagt cgccatcggt
     1381 gctgatcgag ttgggatata tgagcaatgc cgaggacgcc aagctgctcg tctcgcgcga
     1441 ctggcaaagg caggtggcgg gctcgattgc ggccgcggtc gatgaatatt ttctcgagcg
     1501 cggcggcgaa cggcgctgag acggcagcgg caactcgcgc gtttctgtta agtcaattgc
     1561 caagcgagga aaatcggcct cgtgctgcca cattccatgc taaggtgcgc gagccgtgcc
     1621 gcccgagggc gaaaccgccc ccggcaaggc cgatcgaacg aacacgggcc gtgcggactt
     1681 gagcctgaat gagaccgacg ccgatccttc ctccacctgc tgcgccaaag aagaagcgca
     1741 agcgtcgcag ctggctgtta agcctcttag gcttcggctt cgcctccgcg gtggtgatgt
     1801 tcctggccgc cgccggggtc gtgggtttct tcttctggaa ggcggcgcgc gatctgcccg
     1861 actatgagag cctcgccaag tacgagccgc cggtgatgac gcgcatccat gcgcacgacg
     1921 gcagcctgat ggccgagtac gcgcgcgaga ggcgcatttt cgtgccgatc aacgtcatcc
     1981 ccaagctcgt gatcgccgcc ttcctgtccg ccgaggacaa gcgcttttac gagcacggcg
     2041 gcttggactt tgccggcatc ggccgcgccg tgatcaacaa cttccagaac tggggcaaga
     2101 agcgcccgga gggggcctcg accatcaccc agcaggtcgc caagaacttc ctgctctcga
     2161 gcgagcagaa gctcgatcgc aagctcaagg aagccatcct ggccatccgc atcgagcgtg
     2221 cctactcgaa ggacaagatc ctggagctct acctcaacga gatttacctg gggatcggct
     2281 cctacggcgt ggcggcggcc gcgctcaact atttctcaaa ggagctgagc gagctctcca
     2341 tcgaggaggc cgcctatctc gccgccctgc ccaaggcgcc caacaactac catcccttcc
     2401 gcaagacgcg ggaggccacc gtgcgccgca attggatcat cgatcagatg gcggaggtcg
     2461 gctacatcaa cggcgagcag gcgaggttgg ccaaggccaa gcccttgacc gtcaatatcc
     2521 gtcccttcgg cacccagatc tatgccgccg actatttcgc cgaagaggtg cgccgccgcc
     2581 tgatggagat ggtgggcgag gatggcctct atggccgcgc cgagcgcacc gccattgccg
     2641 atggcaccgt gaacggcggc ctttccgtgc ggaccacgct cgatcccaaa ttgcaaaaga
     2701 cggcgcgccg ggcgctgatc gatggtctcg tcgcctttga tcgcgaaaag ggctggcgcg
     2761 ggcccgtgca gaggatcgag attgccggcg actggggcgc ggccttggcc gccgtcgaag
     2821 ctcccaacga cttggcgccc tggcgcctcg gcgtggtgct cgatgcccaa cgcggcaagg
     2881 cggtggtcgg cctgcgcccg ggtcgccaag ccgacggcag cctggcagct gagcgcgagg
     2941 ccgtcgagat ccccttcgag gagttgaagt gggcacgcac gaaccgaggc gtgcccaagg
     3001 tggtgagcga tgtcgtcaac aatggcgatg ttgtctgggt ggcgcccaag gatgctggca
     3061 acgccgcggg tacttggtcg ctgatgcaaa ttccggaagt gggcggggca attgtcgcca
     3121 tggatcccca caccgggcgc gtgcacgccg tcgtcggcgg gttctctttc gccaccagcc
     3181 agttcgatcg ggccgtgcag gccagacgcc agccgggttc ctccttcaag ccgctggtct
     3241 atgccgccgc catcgacaat ggctataagc cgacgagcat cgtgctggat gcgcccatcg
     3301 aaatcgacca ggggcccggc aaagaggtgt ggcagccgaa aaactacgac ggcgtcaact
     3361 cctacggtcc ctccacgctc cgcaccggta tcgagaaatc gcgcaaccaa atgacggtcc
     3421 ggctggccca ggacatgggc atgccgctga tcgtggaata ttccaagcgc ttcggggtct
     3481 acgacgacct cttgccggtg ctggccatgt cgctcggttc cggcgagacg acgctgctgc
     3541 ggatggtggc tgcctacgcg gagctcgcca atggcggcaa gcaggtgcac gccaccctga
     3601 tcgaccgcat ccaggaccgc tggggccgca ccatctggcg ccacgacaat cgcgagtgca
     3661 cgggctgcaa ggttgacaac tggcagggtc aacccgaacc cgtgctggcc gacgatcgca
     3721 agcagatcct cgatccgcac accgcctacc agatgacctc gatcatggaa ggggtggtgc
     3781 agcgtggcac ggccaccatc atcaaaaagt acatccccaa cgtgcccgtg gccggcaaga
     3841 ccggcaccag caacgaggag aaggacgcct ggttcatcgg ctacacgccc gatttggtgg
     3901 tgggcatctt cgtcggctat gacacgccgc gtcccatggg caagggcatg accggcggcc
     3961 acgtcgcggc ccccatcttt ggcgacttca tgcgcatggt gctcgccgac aagaaggcgg
     4021 tccccttccg catcccgccg ggcatcaagc tggtgcgcgt cagcctgcgc acgggcatgc
     4081 ggacccaggg tggcgaagcc gatagcgtgc tcgaagcctt caagccctac gaggacccgg
     4141 acgatcccta ctcgatcgtc ggcttcacca acgagagcgg cggctttttc atgggtgacc
     4201 agcaggacgc
//
LOCUS       DASTMT010000574         3176 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_6100407, whole genome shotgun sequence.
ACCESSION   DASTMT010000574 DASTMT010000000
VERSION     DASTMT010000574.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3176)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3176
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6100407"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1592)
                     /locus_tag="VFR00_13085"
     CDS             complement(<1..1592)
                     /locus_tag="VFR00_13085"
                     /inference="COORDINATES: protein motif:HMM:NF036723.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEU0160249.1"
                     /translation="MNAEAGKPSYTASASPDSRGLDFYGSDPSLKGMLQIYLSAAERA
                     HMEPHFERLGRLVGGRLDDLAGEADKTPPRLEPRTRQGEDRQSIVKAPAYLAMERIAF
                     GELALAALSHRPALGWSRPLSAASKYALTYLFAQSEFGLLCPVNMTDSLTRTIRRFAD
                     ESLLARYLPGLLADETEQQLQGAMFMTERWAGSDVGATETRAVRSGDHWLLYGDKWFC
                     SNADADLALVLARPEGAASGTAGLGLFLMPRLLPTGAPNSYRIVRLKEKLGTRAMASG
                     EVVLAGAAAWLVGDLSQGFKQMAEMVNQSRLSNGVRSTGMMRRAVHEALTVALGRHAF
                     GQRLIELPLVRRQLIKMILPAEAALSVAFFTADCLERADLGDRQAQTLRRILTPLIKF
                     RACRDARKVTGDAMEMRGGNGYIEEWIEPRLLRESHLGSIWEGTSNIIALDVVRAAGK
                     TGAHRLLEAQLRVMLAPAPSDLAAALGERLSAALALIERAGAEAAAARQAASALYYAT
                     AAALLVREAALLQDPVREDMARL"
     gene            1634..2611
                     /locus_tag="VFR00_13090"
     CDS             1634..2611
                     /locus_tag="VFR00_13090"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEU0160250.1"
                     /translation="MHYTTLGKTQLKVSVAGLGCGGNSRLGLGTGLSEGQSVALVREA
                     LDLGVNLLDTAAAYGTEVVVGKAIKGRPRDSVVISTKSHVSVDGRPLSGAEVVANLET
                     SLRQLDTDYVDVFHLHGLAPKAYDHAMAEIVPPLLRQKEKGKLRHLAVSETGPRDPEH
                     KMLERASEEGVWEVVMLAFHMMNQNARQPVLERTRANGIGTLCMFAVRNIFSKPGLLQ
                     ATVAALMAEGKLGQDAIAADDPLGFLVHPGGAESIVDAAYRFARHEPGIDVVLFGTGN
                     AQHLKSNIASILRPALPAPDQARLARLFGHLVGVGLDLPDRMREQVAKR"
     gene            complement(2631..3089)
                     /locus_tag="VFR00_13095"
     CDS             complement(2631..3089)
                     /locus_tag="VFR00_13095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003613209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HEU0160251.1"
                     /translation="MPSKRTVSRPADRVALKRVYAAVSAQDGRRILVDRLWPRGLSKD
                     KAKIDIWLKDIAPSDGLRRRVHGGQEAWSAFVVDYGKELALEPAASAARQLLERIGHE
                     PVTLLYAARNETQNNAVALKTWLIGKLRAGGPPGSATSPADKKKAKAVKP"
BASE COUNT          531 a         1068 c         1034 g          543 t
ORIGIN      
        1 agtcgggcca tatcctcgcg cacgggatcc tgcaacagcg ccgcctcgcg cacgagcagc
       61 gccgccgccg tggcatagta aagtgcgctt gcggcttggc gtgccgccgc cgcctcggct
      121 cccgcgcgct cgatcagcgc cagcgccgcg ctcagccgtt cgccgagcgc cgccgcaaga
      181 tcgctcggtg ccggtgccag catcaccctg agctgcgcct ccagcaggcg atgggcgccc
      241 gtcttgccgg cggcgcgcac gacgtcgagt gcgatgatgt tgctggttcc ctcccagatc
      301 gagccaaggt gggactcgcg caacagcctg ggctcgatcc actcctcgat gtaaccgttg
      361 ccgccacgca tttccatggc atcgcctgtg accttgcgcg catcacggca ggccctgaac
      421 ttgatcagcg gcgtgagaat gcgccgcagc gtctgtgctt gccgatcgcc caaatcggcg
      481 cgttccagac agtcggcggt gaaaaacgcc acgctgaggg cggcctcggc cggcaggatc
      541 attttgatga gttgtcggcg caccagcggc agctcaatca agcgttgacc aaaggcgtgc
      601 cggccgagcg ccacggtcaa ggcctcgtgc acggcgcgcc gcatcatgcc ggtggagcgc
      661 acgccgttcg acaagcgtga ctggttcacc atttcggcca tctgtttgaa accctggctg
      721 agatcgccga ccagccaggc cgcggcaccc gccagcacga cctcacccga ggccatggcc
      781 cgggtgccga gtttctcctt gagccgcaca atgcgatagc tgttgggtgc gcccgtgggt
      841 agcaggcgcg gcatcaagaa caggccgagc cccgcggtcc cgctcgccgc cccctccggc
      901 ctggcgagca ccagcgccaa gtccgcatcc gcgttcgagc aaaaccactt gtcaccgtag
      961 agcagccaat ggtcgcccga gcgtaccgcg cgcgtctcgg tggcgcccac atcggagccg
     1021 gcccaacgct ccgtcatgaa catggcgccc tgcaattgct gttcagtctc gtcggcgagc
     1081 aggcccggca aatagcgcgc aagcaggctt tcgtcggcga agcggcggat ggtgcgggtc
     1141 aggctatccg tcatattgac ggggcagagc aggccgaatt cggactgcgc gaacagatag
     1201 gtgagcgcgt acttgctcgc cgccgagagc ggtctgctcc agccgagcgc cggccggtgc
     1261 gagagcgcgg ccaatgccaa ttcaccgaag gcgatgcgct ccatggcaag ataggccggg
     1321 gccttgacaa tggactggcg atcctcgccc tggcgggtac gcggctcgag gcgcggcggc
     1381 gtcttgtcgg cctcgcctgc cagatcgtcc agccgcccac ccaccagccg gcctaggcgc
     1441 tcaaagtgcg gctccatgtg cgcgcgctcg gcagcggaaa gataaatctg cagcatgccc
     1501 ttgagggatg gatcggagcc gtagaagtcg agcccgcgag agtccggcga ggccgacgcg
     1561 gtgtaactgg gcttgcctgc ttcagcgttc atggccttta atcaccaact ggcgcagcac
     1621 aggttcaaag accatgcact ataccacgct tgggaagacg caattgaagg tgagcgtggc
     1681 cgggctcggt tgcggcggca atagccggct cgggcttggc accgggctca gcgaaggcca
     1741 gtcggtggcc ctggtgcggg aagccctcga tctcggcgtc aatctgttgg atacggcggc
     1801 ggcctatgga acggaagtcg tcgtcggcaa ggccatcaag ggacggccgc gcgacagcgt
     1861 tgtcatctcc acaaaaagcc acgtcagcgt cgacggtcgc ccgctttccg gcgctgaggt
     1921 ggtcgccaat ctggaaacct cgttgcgcca gctcgatacc gactatgtcg acgtctttca
     1981 tctgcacggc ctggcgccga aggcctatga ccatgccatg gccgagatcg tgccgcctct
     2041 cctgcggcaa aaggagaagg gcaagttgcg ccaccttgcc gtctctgaaa cgggtccgcg
     2101 cgatcccgag cacaagatgc tcgagcgcgc cagcgaggaa ggcgtgtggg aggtcgtcat
     2161 gctggccttc cacatgatga accagaacgc gcgccagccg gtgctggagc gcacgcgcgc
     2221 gaacggcatc ggcacgctct gcatgtttgc ggtgcgcaat atcttcagca aacccggctt
     2281 gctgcaggct accgtcgcgg cgctgatggc ggagggcaag ctcggccaag acgccatcgc
     2341 ggccgatgac ccgctcggct tcctggtgca tcccggcggg gcagaaagca tcgtcgatgc
     2401 ggcctaccgc ttcgcccgcc acgagcccgg cattgatgtc gtgctgtttg gcaccggcaa
     2461 cgcccagcac ctcaagagca atatcgcctc gatcctgcgc ccggccctgc ccgcgcccga
     2521 ccaggcgcgc ttggctcgcc tgttcggaca tctggtggga gttggcctcg acctgcccga
     2581 ccgcatgcgc gagcaagtgg caaagcgctg agggcgcttc gcccaggcgc ttaaggcttg
     2641 accgccttgg cttttttctt gtcggcgggc gatgtcgctg agcctggtgg gccgccggcg
     2701 cgcaatttgc cgatcagcca ggtcttgagg gcgacggcgt tgttttgtgt ctcgttgcgc
     2761 gccgcataga gcagcgtcac cggttcgtgg ccgatgcgct cgagcagttg gcgcgcggcg
     2821 ctggccgcgg gttctaaggc cagctccttg ccgtaatcga ccacgaaggc gctccaggcc
     2881 tcttgcccgc cgtgcacacg ccgacgcagc ccatcgcttg gcgcgatgtc cttgagccat
     2941 atatcaattt tcgccttgtc cttgctcagt ccgcgcggcc acagacgatc gaccagaatg
     3001 cgcctgccat cctgtgccga gacggcagca tagacgcgtt tcagcgcgac gcgatcagcc
     3061 ggcctcgaga cggtacgctt gctaggcatg ctggcgttcc cggctttccc tgccgggcga
     3121 cctttaaagg cgccgcacct attgtcggcc cgcagcgcaa agtggcggtg gtcgcc
//
LOCUS       DASTMT010000575         2758 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_5756328, whole genome shotgun sequence.
ACCESSION   DASTMT010000575 DASTMT010000000
VERSION     DASTMT010000575.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2758
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5756328"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..379)
                     /gene="apaG"
                     /locus_tag="VFR00_13100"
     CDS             complement(<1..379)
                     /gene="apaG"
                     /locus_tag="VFR00_13100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012336173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HEU0160252.1"
                     /translation="MYEAVTRGIRIRVTPQFLEQDSSPEQGRYLWAYTIDICNEGSQS
                     VQLRSRHWRITDALGRTQDVRGAGVVGQTPVLAPGASFRYTSGCPLPTPSGIMAGSYE
                     MTSAAGEHFQVTIPTFSLDSPHDE"
     gene            complement(415..1632)
                     /gene="metZ"
                     /locus_tag="VFR00_13105"
     CDS             complement(415..1632)
                     /gene="metZ"
                     /locus_tag="VFR00_13105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008972615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-succinylhomoserine sulfhydrylase"
                     /protein_id="HEU0160253.1"
                     /translation="MSTIPKTSFAADLEGLSPETLLVHGGTLRSGFGELSEAIFATQS
                     YVYQSAEEAEERFKSEAGFIYSRYANPTVAMFEERMRLLEGAEAVRATATGMAAVTAA
                     LLCFLKAGDHLVAARALFGSCRYVVEDLCPRFGIASTLIDGRDTEAWAKALRPNTKAF
                     FFETPANPTLDLVDIAGVCEIARGAGARVVVDNVFATPILQKPLNLGADVVVYSATKH
                     IDGQGRCLGGVVLGSAAYVKDHLHNFLKHTGPSLSPFNAWVLLKGLETLPLRVRAQCA
                     AAAQVADYLARQPGIARVLYCGRPDHPQAGLAKRQMSGFGQVVTFELEAGKPAAFRFL
                     NALRLVKISNNLGDAKSIITHPATTTHQRLKPQARVELGIAEGTLRLSVGLEAMADLT
                     ADIDRARAASRIG"
     regulatory      complement(1725..1802)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00521"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00521"
     gene            1876..>2758
                     /locus_tag="VFR00_13110"
     CDS             1876..>2758
                     /locus_tag="VFR00_13110"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2'-deoxycytidine 5'-triphosphate deaminase"
                     /protein_id="HEU0160254.1"
                     /translation="MAKSAAKPLTRAGASPTGILPVQAIRGLIAAGQVRLEAALASDQ
                     LQPASLDLRLGARAFRVRASFLPGPGRRVSDKLADLQLHTIDLEGGAVLETGCVYIAP
                     LLESMALPDSLGAATNPKSSTGRLDVFTRVIADGVAAFDQIPAGYRGPLYAEVCPQTF
                     PVVVRKGSRLSQVRFRLGAAATSDLALVELNRREKLVSAENADIAAGIALSVDLGGNG
                     QDLIGYRAKRHTGLVDVDVPGACRIADFWEPIYADDSRRLILDPDQFYILASKEAVHV
                     PPTHAAEMVPFNPLVGEF"
BASE COUNT          475 a          923 c          892 g          468 t
ORIGIN      
        1 gctcatcgtg cggactgtcg agcgaaaacg ttggaattgt tacctggaag tgctcacccg
       61 ccgcactcgt catttcgtag ctgcccgcca tgatgcccga aggcgtcggc agcggacagc
      121 ccgaggtgta gcgaaacgac gcgccgggcg cgagcacggg ggtctggccg accacgccgg
      181 cgccgcgcac gtcttgggtg cgccctaaag cgtcggtgat gcgccaatgg cgcgagcgca
      241 gctgcaccga ttggctgccc tcgttgcaaa tgtcgatcgt ataggcccac agatagcggc
      301 cctgctcggg ggaggagtcc tgctccagga attggggtgt gacgcggatg cggatcccgc
      361 gcgtaacagc ttcgtacatg gccgcgctcg ccggtgctcg ccgatcctgg cgctttaccc
      421 gattctagag gccgcgcgcg cgcggtcaat gtcggccgtc aaatcggcca tggcctcgag
      481 gcccaccgac aggcgcagcg tcccctcggc gatcccgagt tccacccgcg cctgcggttt
      541 caagcgctgg tgcgtggtgg tcgcgggatg cgtgatgatg ctcttggcat ccccgaggtt
      601 attggaaatc ttcaccaatc gcagggcgtt gagaaagcgg aaggcggccg gcttgccggc
      661 ctcgagttcg aaggtcacga cctggccgaa gcccgacatc tggcgcttgg cgaggccggc
      721 ctgcgggtga tcgggccgac cgcagtagag cacgcgggcg atccccggtt ggcgggcgag
      781 gtagtcggcg acttgggcag ccgcggcgca ctgggcgcgc acccgcaacg gtaacgtctc
      841 caagcccttc aacaagaccc aagcattgaa ggggctgagc gaggggccgg tgtgtttcaa
      901 gaagttgtgt aaatgatcct tgacgtaggc ggcggagccc agcaccacgc cgccgaggca
      961 gcgaccttgc ccgtcgatgt gcttggtggc cgagtagacg accacgtcgg cgccgagatt
     1021 gagcggcttc tgcaggatcg gcgtggcaaa cacattgtcg acgaccaccc gcgccccggc
     1081 gccgcgcgcg atctcacaca cgcccgcaat gtcgacgaga tcgagcgtcg gattggccgg
     1141 cgtctcgaaa aagaaggcct tggtgttggg gcgcagcgcc ttggcccagg cctcggtgtc
     1201 gcggccatcg atcaaggtgg aggcgatgcc aaagcgtgga cagagatctt cgaccacgta
     1261 gcggcacgag ccaaacagcg cgcgcgcggc aacgaggtga tcgccggcct tgagaaaaca
     1321 caacagcgcc gcggtcacag ccgccatccc cgtcgccgtt gcccgcaccg cctccgcccc
     1381 ctccagcagc cgcatgcgtt cttcgaacat cgctacggtc ggattggcgt agcggctata
     1441 gataaagccc gcctcactct tgaagcgttc ctcggcttcc tcggcggatt gatagacata
     1501 gctttgcgtg gcgaatatcg cctcggacag ctcgccgaaa cccgagcgca gcgtgccgcc
     1561 gtgcaccaat agggtttccg ggctcagccc ctcgaggtca gccgcgaagc tcgttttcgg
     1621 gattgtcgac atcggcgctc tccctggcct ggcggcaagg ccaggcaaca aaaaaccggc
     1681 ttgactcgac gccgcttggc atgccgggcg ctctcatctc gtcggtcggg tcgcgcacgg
     1741 ccttttagcg acttgtttta cgtggctgca agccggccgg ccaaatgacc acgtctcaga
     1801 gaccggggat taccgcgagc gggcgcttgg cgtcaatggg tctatcgtct agacggacgg
     1861 caaacgaggc agtcgatggc caaatctgcg gcgaaaccgc tcacccgcgc gggcgcgagc
     1921 ccgaccggca ttttgcccgt acaggccatc cgcggcctga tcgcggccgg ccaggtgcgg
     1981 ctcgaggcgg cgctggccag cgaccagctg cagcccgcca gccttgactt gcgcctgggc
     2041 gcacgggcct ttcgcgtgcg cgcctcgttt ctgcctggcc cgggccgccg ggtcagcgac
     2101 aaactggcag acctgcaact gcacaccatc gacctggaag gcggcgccgt cttggaaact
     2161 ggctgcgtct acatcgcgcc gctgctcgag agcatggcgc tgcccgacag cttgggcgcc
     2221 gccaccaatc caaaaagctc gaccggacgc ctcgacgtct tcacccgcgt cattgccgac
     2281 ggcgttgcgg ccttcgatca gatacccgcc ggctatcgag ggccgcttta cgccgaggtc
     2341 tgcccgcaga cctttccggt ggtcgtgcgt aaaggctcgc gcctgagcca ggtacgcttt
     2401 cggctcggag ccgccgccac cagcgatctt gccctcgtcg aactcaatcg gcgcgaaaag
     2461 ctggtctcgg cagaaaacgc cgatattgcc gccgggattg cgctctccgt cgatctgggc
     2521 ggcaacgggc aagacctgat cggctaccgc gccaaacgcc acacagggct cgtcgacgtg
     2581 gacgtcccag gcgcctgccg catcgccgat ttttgggaac ccatttacgc cgacgacagc
     2641 cgtcggctca ttctcgaccc cgatcagttc tatatcctcg cctccaagga ggcggtacac
     2701 gtgccgccca cccatgccgc cgagatggtg cccttcaatc cgctggtcgg cgagttcc
//
LOCUS       DASTMT010000576         4357 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_3798629, whole genome shotgun sequence.
ACCESSION   DASTMT010000576 DASTMT010000000
VERSION     DASTMT010000576.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4357)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4357
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3798629"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(12..386)
                     /locus_tag="VFR00_13115"
     CDS             complement(12..386)
                     /locus_tag="VFR00_13115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0160255.1"
                     /translation="MARLASLARRGLGQIGWPATNGPPDVLPLVQAWSLPNLPALQKL
                     GLGPVWAVRCPFCRDFHTHLPGEGKRQAACSLDAIPAAYALGYAGDLPRVLHDAFRLS
                     IGQAWPKVLLEWHEGAASLQAA"
     gene            723..1097
                     /locus_tag="VFR00_13120"
     CDS             723..1097
                     /locus_tag="VFR00_13120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016847513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEU0160256.1"
                     /translation="MAGDVEAVERVVWTYLDGLYEGDTAKLAKAFHEVSHLYSLEEGA
                     VSDLPRADWFKLVESRASAKARGLKRTDRIVSIDFSGPETAFVKLECSIHPRYFTDYL
                     TLLKLAQGWRVVSKTFRTERRE"
     gene            complement(1102..2160)
                     /locus_tag="VFR00_13125"
     CDS             complement(1102..2160)
                     /locus_tag="VFR00_13125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008144938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEU0160257.1"
                     /translation="MASWTELPADVLALLRRGTVLPAHPLALDADRRLDRVSQRALAR
                     YYIDAGAGGLAVGVHATQFAIREAGLYRPVLELAAETAQSWTRRPLLLIAGVIGRTAQ
                     ALAEARLARALGYHAVLLGLSQLRGASEDELIEHCRRIAGEVPVIGFYLQTAVGGIAL
                     SRTFWARFASIDNVIAIKVAPFDRYKTLDVAFGVVTAGAQDRITLYTGNDDHIVADLV
                     TPMRIRSPTREVLLRFKGGLLGHWSVWTKPAVALLERAHAAAGVGAVPDDLLAFDAMV
                     TDCNSVIFDVANGFAGCIAGCHEVLRRQGLMQTRHCLDPNEGLSPGQAEGIDRVTAAY
                     PELNDDAFVSANLARWRS"
     gene            complement(2186..3064)
                     /locus_tag="VFR00_13130"
     CDS             complement(2186..3064)
                     /locus_tag="VFR00_13130"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF037026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU0160258.1"
                     /translation="MATIEKAGLPSIVALQAADAAALCPLSLEAGWNQVAEDWRLMLT
                     LGEGFGARARDGRWIGSALTLPLGPRLAWISMVLVTKPARGQGLGTALLSASLAAAEA
                     SGRAAGLDATEFGRPIYLPLGFRDVFGLARWQLDAPQQTAIAPPPGIRVRAANRDDLA
                     AILAYEPPRSGFCRQAILADLLSRSAHFAHVACAADGALAGFLLGRNGHRHWHLGPIV
                     ADAEATALALLSGAAAQLGRPLIVDVPEQHGRMRDWLLAQGATAPRRFSRMLRGMTGK
                     SNAPDGVFALAGPELG"
     gene            complement(3051..4097)
                     /locus_tag="VFR00_13135"
     CDS             complement(3051..4097)
                     /locus_tag="VFR00_13135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019171792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU0160259.1"
                     /translation="MMVFSAAHPLPAGFQSVVELENFLSEPPPEVIGDLARVHGDILV
                     LGVAGKMGPTLARMAKRAAPDKRVVGVARFSDPAVRAGLAAAGIETIACDLMDRDALS
                     QLPRLPNVVYMAAMKFGASGNPALTWAMNVHVPALVAETFQSSRIVAFSTGCVYPFVP
                     VDSGGATEATPAVPPPGDYANSCVGRERMFEFFSARLGTPGRLIRLNYAIDMRYGVLH
                     DIAVKVRDGVPIDLSMGHVNVIWQGDANAVALRCLARVTNPTSPINVTGPEILRVQQL
                     AEALGRRLQREPRFSGAEARTGWLNNAGRMLAEFGPPRVPIEAMLEWTADWLARGMVS
                     HGKPTHYEVRDGHY"
     gene            complement(4094..>4357)
                     /locus_tag="VFR00_13140"
     CDS             complement(4094..>4357)
                     /locus_tag="VFR00_13140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007692228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0160260.1"
                     /translation="VERNGHHFVDGMSFAPPREQEDFARAHPDLYEQRDGVTRLAIEA
                     GRLRLASLDCAGFAVAAPMDFTCLRAMPAETLSPLVPAHPERA"
BASE COUNT          813 a         1442 c         1462 g          640 t
ORIGIN      
        1 ctgacaaagc ctcaagccgc ctgcagcgag gccgcgcctt cgtgccactc cagcagcact
       61 ttcggccagg cctggccgat gctcaagcga aaggcatcgt gcagcacgcg gggtaaatct
      121 cccgcgtagc caagcgcgta ggccgccggg atcgcgtcca gcgagcaggc cgcctggcgc
      181 ttgccttcac cgggcagatg cgtgtggaag tcgcggcaga aggggcagcg cacggcccag
      241 actggaccca gccccaactt ttgcaaggcc ggcaggttcg gtagcgacca ggcctgcacg
      301 agtggcagaa cgtccggcgg cccgttcgtg gcgggccacc cgatctggcc aagcccgcgc
      361 ctagcgaggc ttgcgagccg cgccatgact ccattgagcc tggtaggctt gggccgaccc
      421 tcacagacgg cggcgagcgc acggccctcg aaaaagcgcg ccagccagaa ccacagtgtc
      481 ccccaccgcc gcaaggcccc acccagccag tgaggaagac accggtcttg cgcggcagcg
      541 aacgcgacct tggaaacccc ccaggcgatt ttttcaagca tggttcaaga cccctggttt
      601 gccccccagg cgcccgaggc ggaacgttgg accgccgatc gtaaacaaat ggttaacgac
      661 tgtccccaag cgcggcgaaa gcggttatcc tccgaggcat tagaacgtgt ggaggagtgg
      721 ccatggcggg tgacgtcgaa gcggtcgagc gtgtggtgtg gacctatctc gacgggcttt
      781 atgagggcga tacggccaag ttggccaagg ccttccacga ggtgagccac ctctacagtc
      841 tcgaggaggg cgcggttagc gacctaccgc gcgccgattg gttcaagctg gtggagagcc
      901 gtgcctccgc caaggcccgc ggtctcaaac gcaccgaccg catcgtttcc atcgactttt
      961 ccggacccga gaccgccttc gtcaagctcg aatgctcgat ccatccgcgc tacttcacgg
     1021 attacctgac gctgctgaag ttggcgcagg gctggcgcgt ggtgtccaaa accttccgca
     1081 ccgagcggcg cgagtagcgc ctcagctgcg ccagcgtgcg agattggcgc tgacgaaggc
     1141 gtcatcatta agctcgggat aggccgcggt aacccgatcg atgccctcgg cctgtcccgg
     1201 actgaggccc tcgttcgggt cgagacagtg acgggtctgc atcaagcctt gacgtcgcag
     1261 cacctcgtgg cagccggcga tacaaccggc aaagccgttg gccacgtcga aaatcacgct
     1321 gttgcaatcg gtgaccatgg catcgaaggc gagcaggtcg tcaggcaccg caccgacgcc
     1381 ggccgcggca tgcgcgcgtt ccaagagcgc gacggcaggc ttggtccaga cgctccagtg
     1441 cccgagcagc ccgcctttga agcgcaacag cacctcgcgc gtcgggctgc ggatgcgcat
     1501 gggcgtgacg agatcggcaa cgatgtggtc atcgttgccg gtataaagcg taatgcgatc
     1561 ctgagcccct gcggtcacca cgccaaaggc gacatcaagc gtcttgtagc ggtcgaacgg
     1621 cgccaccttg atggcaatca cgttatcgat cgaggcaaaa cgcgcccaga aggtgcgtga
     1681 cagggcgatg ccgccgaccg cggtttgtaa gtaaaagccg atcacgggca cttcgcccgc
     1741 aatgcgacgg caatgctcga tcagctcgtc ctcgctggcg ccgcgcagct gagacagacc
     1801 caacagaacc gcgtgataac cgagcgcacg ggcgagccgc gcctcggcga gcgcctgcgc
     1861 cgtcctgccg atcacgccgg cgatcaacag caggggccgg cgagtccagc tttgcgccgt
     1921 ctcggcggcc agttcgagga cgggacgata gaggcccgcc tcgcggatgg cgaattgggt
     1981 ggcgtgcacg cccacagcga gcccgccggc gcccgcatcg atgtagtagc gcgctagcgc
     2041 gcgctgcgac acgcgatcga gccgtcgatc cgcatcgagc gccaacggat gcgcgggtag
     2101 tacggtgccg cgccgcagaa gggcgagaac atcggcgggc agctctgtcc aggacgccat
     2161 ggcaaaggat ccttgttggc gtaggctagc cgagctcggg ccctgccagc gcgaacacac
     2221 cgtccggagc attgctcttg cccgtcatgc cccgcagcat gcggctgaag cggcgggggg
     2281 cggttgcgcc ttgcgccaac agccagtctc gcatacggcc atgctgctcg ggaacatcga
     2341 caatcaaggg ccgccccagt tgggcggcgg cgccgctcag caaggcgagc gccgtggctt
     2401 ccgcgtccgc aacgatgggc ccgaggtgcc agtgacgatg gccattgcgg ccaagtagaa
     2461 agcccgccaa ggccccgtcg gccgcgcagg cgacgtgcgc aaagtgagcc gagcgggaca
     2521 acaggtcggc aaggatcgcc tggcggcaaa aaccgctgcg cggcggctcg tatgcgagga
     2581 tggcggcaag gtcgtcgcgg ttggcggcac gcacgcggat accgggcggc ggggcgatgg
     2641 cggtttgctg cggggcgtcc agctgccagc gtgccaggcc aaagacgtcg cggaagccca
     2701 ggggcaaata gatcgggcgc ccaaactcgg tggcatcgag ccccgccgcg cggccgctgg
     2761 cttcggcggc ggcgagcgaa gccgacaaca gggcggtgcc aagaccttga ccgcgcgccg
     2821 gttttgttac caacaccatg ctgatccagg cgagccttgg ccccagtggc agggtcagcg
     2881 cgctgccgat ccagcgccca tcccgcgctc gcgcaccaaa accctcaccc aatgtcagca
     2941 tcagccgcca gtcctcggcg acctggttcc agccggcctc aagggaaagc gggcaaaggg
     3001 ccgcggcgtc ggccgcctgc agtgcgacga tgcttggcag cccagccttt tcaatagtgg
     3061 ccatcgcgca cctcatagtg cgttggcttg ccgtgactga ccatgccgcg cgcgagccag
     3121 tcggcggtcc actccagcat ggcctcgatc ggcacgcgcg gcggtccaaa ctcggccagc
     3181 atgcgtccgg cgttgttgag ccatcccgtg cgcgcttccg cgccgctgaa gcgcggctcg
     3241 cgctgcaagc gccggccgag ggcctcggcc agctgttgca cccgcaagat ctcaggcccc
     3301 gtcacattga tgggactggt gggattggtg acgcgcgcca gacagcgcag cgccaccgcg
     3361 ttggcatccc cctgccagat aacgttgaca tggcccatgc taagatcgat gggcacacca
     3421 tcgcgcacct tgacggcgat gtcgtgcaac acgccgtagc gcatgtcgat ggcatagttg
     3481 agccggatca agcgtcccgg ggtgccaagc cgggcggaaa aaaattcgaa catgcgctcg
     3541 cggccgacgc aggaattggc gtagtccccc ggcggaggca cggccggcgt cgcctccgtc
     3601 gccccaccgc tgtcgacggg cacgaatgga tagacgcagc cggtggagaa ggcgacgatg
     3661 cgggaggatt ggaacgtttc ggccaccagg gcgggaacgt gcacgttcat cgcccaggtc
     3721 aaggctggat tgccgctggc gccaaacttc atggcggcca tgtagacgac attggggagt
     3781 cgcggcagct ggctcagcgc gtcacggtcc ataagatcac aggcaatggt ctcgatgccg
     3841 gccgctgcaa gtccggcgcg cacggcgggg tcggagaagc gggcgacccc gaccacccgc
     3901 ttgtccggag ccgcgcgttt tgccatgcgc gccagcgttg gacccatctt cccagcaacg
     3961 ccgagcacga gaatgtcgcc gtgcacgcgc gcgagatcgc cgatcacttc cggtggcggc
     4021 tcgctcagga agttctcgag ctcgaccacg gattggaacc ctgcgggcag ggggtgcgcg
     4081 gcagaaaaga ccatcatgct ctctcgggat gagcgggcac gagcggcgag agggtctcag
     4141 ccggcatcgc ccgcaggcaa gtgaaatcca tcggcgccgc gaccgcgaag ccggcgcaat
     4201 cgagcgatgc caggcgcagt cgtccggcct caatggcaag ccgcgtcacg ccatcgcgtt
     4261 gctcgtaaag atcggggtgc gcgcgggcaa aatcctcctg ctcgcgaggc ggcgcaaacg
     4321 acataccatc gacgaagtga tgaccgttgc gctcgac
//
LOCUS       DASTMT010000577         8159 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_5447957, whole genome shotgun sequence.
ACCESSION   DASTMT010000577 DASTMT010000000
VERSION     DASTMT010000577.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 8159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8159
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5447957"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..213)
                     /locus_tag="VFR00_13145"
     CDS             complement(<1..213)
                     /locus_tag="VFR00_13145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HEU0160261.1"
                     /translation="MPNRPVFRFAPSPNGELHLGHALSALIGHERAAALGGRFLLRIE
                     DIDAERSRAQFIAGIFEDLGWLGLSWP"
     gene            455..1117
                     /locus_tag="VFR00_13150"
     CDS             455..1117
                     /locus_tag="VFR00_13150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015928527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2 family
                     protein"
                     /protein_id="HEU0160262.1"
                     /translation="MPRTAAVPRIIASEEDVREGVRALRRRCPTMRMVHDAAGDPPLR
                     RRPAGFEGLARIIVGQQLSVASASAIWLRTLNLCQPFAPAGVEALDDAVLASAGLSRA
                     KIRTLRAMALACSNGLELAKLEAQSDTEIATALTAINGIGPWTADIYIMFCLGRGDAF
                     AAGDLALQIGAQQALGLKMRPSAEELLAIAERWRPWRGVAARLLWNYYAHLKRTGRAA
                     PV"
     gene            1117..1779
                     /locus_tag="VFR00_13155"
     CDS             1117..1779
                     /locus_tag="VFR00_13155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012334595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEU0160263.1"
                     /translation="MAFGTIKALDGPRLPPVRGRANALIVLLHGYGANGDDLIGLAQG
                     WRARLPGAAFVAPNAPESIPGMPGALQWFALSLRDPHEFWQGVVAAAPGIDRFIDQEL
                     ARLSLGADRLALVGFSQGTMLALHVGLRRGQAPAAIVGYSGLLAGPERLAEMKASPPV
                     LLVHGETDDLIPVEALHLAREQLAAKAVRVEWHVRSGLAHGIDAGAQWLGAHFLAEAL
                     HA"
     gene            1926..2486
                     /locus_tag="VFR00_13160"
     CDS             1926..2486
                     /locus_tag="VFR00_13160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEU0160264.1"
                     /translation="MNAYAAAPENFPALVLNADFRPLSYYPLSLWSWQESVKAVFLER
                     VNIVSEYERFVRSPSFEMRLPSVVSLKTYVKPALYPAFTRFNVFLRDRFACQYCGGKD
                     DLTFDHLIPRSRGGQTRWDNVVTACAPCNLSKGGHLAHHAGMQPRQMPYRPTVFELHR
                     NGRLFPPNYLHESWLDYLYWDTELEP"
     gene            complement(2526..2735)
                     /locus_tag="VFR00_13165"
     CDS             complement(2526..2735)
                     /locus_tag="VFR00_13165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0160265.1"
                     /translation="MFPRHVNPMQWQLAMDYARQACARIFRDGGSAAEALRAFDLPAE
                     GTPNWNTSVSRIAEALCVSTQRKAA"
     gene            complement(2910..3062)
                     /locus_tag="VFR00_13170"
     CDS             complement(2910..3062)
                     /locus_tag="VFR00_13170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0160266.1"
                     /translation="MSVHEETFVPVRPGPPWVALSDAAAAIVRALPTPQPGHAAQPPC
                     QPGRDD"
     gene            3642..4292
                     /locus_tag="VFR00_13175"
     CDS             3642..4292
                     /locus_tag="VFR00_13175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta family hydrolase"
                     /protein_id="HEU0160267.1"
                     /translation="MLDFLVAGPPHPTARYLCAHGAGAGMATPFMVAIAAALAERGIA
                     TLRFEFAYMAARRASGTKKPPPRAERLLEEYVAAARAAPKDAPLFIGGKSMGGRVASL
                     IADRLWDEGLIAGLICLGYPFHPPKRPAELRTAHLAGLRCPALIIQGERDPFGSRAEV
                     EGYALSRAIRLVWAADGDHDLGPRGASGHTRRGNLAAAAAAIAAFAQSERPTGETA"
     gene            complement(4347..5456)
                     /locus_tag="VFR00_13180"
     CDS             complement(4347..5456)
                     /locus_tag="VFR00_13180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011580411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HEU0160268.1"
                     /translation="MKIDAVELTLFAWDDIPPTKYTHGTQNLSGHSSLGLLRLRTDAG
                     IDGHAFLGSATNPAETDAGALMRFLKPLLLGKDPLGREELHAAMRGRQRHTGLRAIGA
                     CDTALWDIAAKAAGMPLNVFLGSTRSAIGAYASSQVLDSVAAYAEEAAQFRADGWKAY
                     KIHPPHGPELDIEVCEAVRKAVGDDYTLMLDSTWSYRYPEALKVGRAIEGLGFLWYED
                     PLNEEDIYAYVKLAQKLDIPILATEYPAGDIGTYAVWLTERATDYLRGDIPQKGGLTT
                     MLKTAHLAEAFHMNYEVHHGGNSLNNMAQLHFACAIRNTTYFEVLLPHGAHKYGLARD
                     IEIGKDGLAHCPTGPGIGAEIDFALIERKAMARLS"
     gene            complement(5589..6020)
                     /locus_tag="VFR00_13185"
     CDS             complement(5589..6020)
                     /locus_tag="VFR00_13185"
                     /inference="COORDINATES: protein motif:HMM:NF016060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HEU0160269.1"
                     /translation="MQRFASPTTGARRSRLRHGLAFLLSGGLAFMADAVTLKALTVLV
                     GLNPMLARLFAISLAMLVGWLAHRTFTFALQVPPSLAEFLRYAVVGWSVAAVNYGLFV
                     LILLLWPAAEPLLALIVSSLFATLFAYLGMRYGAFRVERGG"
     gene            complement(6025..6879)
                     /locus_tag="VFR00_13190"
     CDS             complement(6025..6879)
                     /locus_tag="VFR00_13190"
                     /inference="COORDINATES: protein motif:HMM:NF016668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HEU0160270.1"
                     /translation="MLILCADDYALSSGVARAIGELSAARRLSATSVLVTAAHWPGLA
                     PRLSVHRGHLALGLHFNLTLGAPLGRMPRLAPTGTLPSLAKLVGASLLGLVDRSEIRD
                     EFERQLDHFERGLACPPDQVDGHHHVHVLPGIREVVLDTLARRYPTLPPLVRDPSDSW
                     EAIAARRIAKGKAALVATLAAGFGAAAQRRGLPTNAGFSGFSNFDAKVPYASEFAQAL
                     QKLGPRPIVMCHPGHPDAGPGHGDGVTARRRMEYDALMGDRALPQRIWRPARPPGGPP
                     VDWASVRF"
     gene            complement(6922..7953)
                     /locus_tag="VFR00_13195"
     CDS             complement(6922..7953)
                     /locus_tag="VFR00_13195"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018266105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU0160271.1"
                     /translation="MSIPDPDAAAPLPREARPARGLISVVIPAFNEAAGLDDLLARLQ
                     PALAGLGTDWEIIFVDDGSSDNTRDKLKALNAGDRRLKGLVLSRNFGKESAMAAGLRY
                     AQGDAVIVMDADLQHPPELIAAFVQYWQQGDDIVYGQRLDRAADSLAHRWAARVYYAA
                     FRKLTRTVLPAGAGDFRLLDRKAVDAINSMGERARFTKGLYAWIGFRARGVPFQVPAR
                     LAGKSRWRPRELLRFGIDGLASFTTIPLKVWSYVGLLISLFAFCYAMIFLIKTLIYGT
                     DVPGFPTLVISVMFFAGVQLLSLGIIGEYLGRVYEEVKGRPLFLVAEELGLEKAARPP
                     RRTNAKGEA"
BASE COUNT         1376 a         2668 c         2792 g         1323 t
ORIGIN      
        1 gggccaggag agcccgagcc agcccaggtc ctcgaaaatt ccggcgatga actgggcccg
       61 gctgcgttcg gcgtcgatat cctcgatgcg cagaaggaag cggccaccga gcgccgcggc
      121 gcgctcgtgg ccgatcaggg cagagagcgc gtgcccgagg tgcagttcgc cgttgggact
      181 gggggcaaat cggaagaccg ggcggtttgg catgacatcc cagcgcctgg gcacaggcgc
      241 cgcgggccct tgtgcgcttg ccgcttgagc ttgcgtcaaa gctagtccga ccgctacacc
      301 aaagatgcgc ctgccccagt tggtggggcg cgcgtcccgt gggttggaac ggaaatgagg
      361 ttgcgtgcgc ggcgaggctg agagcgctaa acgacgcgga cgccccggca aagcgccgaa
      421 ggcgccgctt gctgcggccg ctgtcaaggc gcgcgtgccg cgaacggcag ccgtcccgcg
      481 catcattgcc agcgaggagg acgtgcgcga gggcgtccgc gcgctacggc gccgctgccc
      541 caccatgcgc atggtgcacg atgccgcggg cgatccgcca ttgcgccggc ggccggcggg
      601 ctttgagggt ttggcccgca tcatcgtggg ccagcagctc tcggtggcaa gcgcctcggc
      661 gatctggctg cgtacgctga acctgtgcca acccttcgca cctgcggggg tcgaggccct
      721 cgatgacgcc gtgctggcgt cggccggatt atcccgtgcc aagattcgca ccctgcgcgc
      781 gatggcgctc gcctgcagca acggcctcga gcttgcgaag cttgaggcgc agagcgatac
      841 cgaaattgcg accgcgctca cggccatcaa cggcattggt ccctggacgg ccgacatcta
      901 cattatgttc tgcctcggtc gcggcgatgc ctttgccgcc ggcgacctcg ccttgcaaat
      961 tggcgcccag caggcgcttg gcctcaagat gcggccgagc gcggaagaat tgctggccat
     1021 cgccgagcgc tggcgaccct ggcgtggggt cgcggcgcgc ctattgtgga actattacgc
     1081 gcacctgaaa cggacgggca gagcggcacc ggtataatgg cgttcggtac gatcaaggcg
     1141 ctcgatggac cgcgtctgcc gcccgtgcgc gggcgggcga acgcgctcat cgtgctgttg
     1201 cacggctatg gggcgaacgg cgatgacctg atcggcttgg cgcagggctg gcgtgcgcgc
     1261 ctccccgggg cagccttcgt ggccccgaac gcgcccgaaa gcattcccgg catgccgggg
     1321 gcgctacagt ggtttgccct ttccttgcgt gatccgcacg aattttggca gggcgtggtg
     1381 gccgccgcgc ccggcattga tcgttttatc gatcaggagc tggcgcggct tagcctcggc
     1441 gccgaccgcc tggccctcgt cggcttcagc cagggcacca tgctcgccct gcacgtgggc
     1501 ctgcgccgcg gacaagcgcc cgcggcgatc gtgggctatt ccgggctgct ggcaggcccg
     1561 gagcgcctcg ccgagatgaa agcatcgccg ccggttctgc tcgtgcacgg cgaaaccgat
     1621 gatctcattc ccgttgaggc cctgcatctg gcgcgtgaac agctggccgc caaagctgtg
     1681 cgcgtcgagt ggcatgtgcg cagcgggctt gcgcacggca tcgatgccgg cgcccagtgg
     1741 ctcggcgcgc acttcctcgc cgaagccttg cacgcctgag gccgcgggcg cggaccgcgt
     1801 tttttgcctg gcttttcaag cacaccccgg tgccttcaca ggcccgacat gcacagctag
     1861 tatggccccg ctcagcgcgc cggggacgtt gattcggtcc ccgacatggt cgagagattg
     1921 gagctgtgaa cgcttacgct gccgcgccgg aaaactttcc ggccctcgtc ctcaatgccg
     1981 acttccgccc cttgagctac tatcccttgt cgctctggag ctggcaggaa tcggtaaagg
     2041 cggtgttttt ggagcgcgtc aacatcgtct ccgaatacga gcggttcgtg cgcagcccct
     2101 ccttcgagat gcggctgccg agcgtggtgt cgctgaaaac ctacgtgaag ccggcgcttt
     2161 atccggcctt tacgcgcttt aacgtgttct tgcgcgaccg ctttgcctgc caatactgcg
     2221 gcggcaagga cgatctcacg ttcgatcacc tgatcccgcg ctcgcgcggc ggtcaaacgc
     2281 gctgggacaa cgtggtgacg gcctgcgccc catgcaattt gagtaagggt gggcatctgg
     2341 cgcaccatgc cggcatgcag ccgcggcaaa tgccctaccg gccgacggtg ttcgaactgc
     2401 accgcaacgg ccggctattc ccgcccaact atctgcatga aagctggctc gactacctct
     2461 actgggacac cgaactcgag ccgtaacgcg atgctacgaa atggcggcgt gaccgaccct
     2521 gagggtcagg ccgccttgcg ttgcgtgctc acgcataagg cctcggcgat gcggctgacc
     2581 gaggtattcc agttgggtgt tccctcggcg ggcaggtcga aggcgcgcaa ggcttcggcg
     2641 gcgctgccac cgtcgcgaaa aatgcgggcg caggcctgac gggcatagtc catcgccagt
     2701 tgccattgca tgggattcac gtggcggggg aacatctctg acaccttccg tgtttgtggt
     2761 ttgttgtggc ttgagctgca gcagggagca ggcgcatcca gcgtcttggt ttcacgacgc
     2821 aaaaccttga cacgcgaaac cttcgttgac tttggtctgt cgcacgctgg atgcgcctgc
     2881 ggcagggacg gtggagcaga cgaccatccc taatcgtccc ggcccggctg gcagggcggc
     2941 tgcgccgcgt ggccgggctg aggcgtgggc agcgcgcgca cgatcgccgc ggccgcgtcg
     3001 ctcaacgcca cccagggcgg gcccggtcga accgggacaa aggtctcctc gtgcacagac
     3061 atggggaggc tgctcctcaa cgcttggggt cctcgggggc ccgccaggct ggcgagccgc
     3121 gccgccaaac gcgcactctt ctttgcggcc gcggctcgcc tcaacgccgc cagggcggac
     3181 ccgaggcttg ctttggggga ctgggctaac tcagacgcaa cagaacgctc gaggacgcaa
     3241 cgcaaccgac gcggctcacg tgtcgggacc cagggactac gccaaacctt cggtccagcc
     3301 gggccgaccc accgagctcg gggggaacgc aacaacgatg ccccgggctc gatcacagga
     3361 tcgtgagctg tggacaaacg caacgcaaca ccgggcaaga ggacgcggcc actgggaaca
     3421 cggggatgca tactcacctc acgacgttca cattgtgttc caggccgggc tctataaaca
     3481 acccccgttt gtttcttgtt tggcacagcg gcggcagaaa agaggccacc gccgatgtcc
     3541 aacataaaat cgaccttgac gcggaggcct cgataggttg cggcgcaagc gaagcgattc
     3601 gcggcccggg gtgccgtgag aagggcaaga aaggcaaggc catgctggac ttcctggtcg
     3661 cggggccgcc gcacccgacg gctcgctatc tgtgcgcgca tggcgcgggg gctggcatgg
     3721 ccacgccctt tatggtggcg atcgcggccg ccctggccga gcgggggatt gccaccttgc
     3781 gattcgaatt cgcctacatg gccgcgcgcc gtgcgagcgg cacgaaaaag ccgccgccgc
     3841 gcgcggaacg gctgctggag gaatacgtgg ctgcagcgcg ggcggcgccc aaggacgccc
     3901 cgctcttcat cggaggcaaa tccatggggg ggcgtgtggc gagcctcatc gccgaccgcc
     3961 tgtgggacga gggactgatc gcggggctga tctgcctcgg ctatcccttc catccgccca
     4021 agcggcccgc cgagttgcgc accgcgcatc ttgccggttt gcgctgtccg gcgctcatta
     4081 ttcagggcga acgtgatccc ttcggcagcc gcgcagaagt cgagggctac gccctctcgc
     4141 gggccattcg cctggtttgg gccgcggacg gggatcatga cttgggcccg cgcggggctt
     4201 ccggccacac gcgccggggc aacctggcgg cggcggcggc agccattgct gccttcgctc
     4261 aaagcgagcg cccgacgggc gagacggcct gaagccctgc tcgctggcca cgcggcggcg
     4321 ggcgccggag tgcaagggcg cgcgcctcaa ctgaggcgtg ccatggcctt gcgctcgatc
     4381 agcgcaaaat cgatttcggc gccgatgccg ggcccggtcg ggcaatgcgc caggccgtcc
     4441 ttgccgatct cgatgtccct cgcaagccca tacttgtgcg cgccgtgtgg caggaggact
     4501 tcgaaatacg tggtgttgcg aatggcacag gcgaagtgca gctgcgccat gttgttcagc
     4561 gaattgccgc catggtgcac ctcgtaattc atatgaaacg cctccgccaa atgcgccgtc
     4621 ttgagcatgg tggtgaggcc gcccttttgc ggaatgtcgc cgcgcaggta atcggtggcg
     4681 cgctcggtca gccacaccgc gtaagtgccg atatcgcccg cgggatactc ggtggcgagg
     4741 atggggatat cgagtttttg cgcgagcttg acgtaggcgt agatgtcctc ctcgttgaga
     4801 gggtcctcgt accacaagaa ccctagcccc tcgatggcgc gccccacctt gagcgcttcg
     4861 ggatagcgat agctccaggt tgaatcgagc atcaaggtgt agtcgtcgcc caccgccttg
     4921 cgcacggcct cgcacacctc gatatcgagc tcgggaccat gcgggggatg gatcttgtag
     4981 gctttccagc catcggcccg aaactgcgcc gcctcctcgg cgtaggcggc gacgctgtcg
     5041 agcacttggc tcgaggcgta ggcgccgatg gccgagcgcg tgctccccag gaagacgttg
     5101 agcggcatgc ccgcggcttt ggcggcgatg tcccagagcg cggtatcgca ggcgccaatg
     5161 gcgcgcaatc ccgtatgccg ttgccggccg cgcatggcgg cgtggagctc ctcacgcccc
     5221 agcgggtcct tgcccaagag gagaggtttg agaaaccgca tcagcgcccc ggcgtcggtc
     5281 tcggccgggt tggtggcgga tcctaggaac gcatggccgt cgatgccggc atcggtgcgc
     5341 agccgcagca gccccaagct gctgtgaccc gataggttct gcgtgccgtg ggtgtacttc
     5401 gtcggtggaa tgtcatccca cgcgaacagg gtgagctcga cggcatcgat cttcatgcgt
     5461 gctacctctt ggcgccctga ttgcgcggcg cgcgcacgca gggggttgcg acggcccggc
     5521 ccagcaaccc agcctagcgc ggcgatccgt ggtctgtcgc cttggcaatt tgcgcccgcg
     5581 cccgcccctc atccgccgcg ctcaacgcga aaggcgccgt agcgcatgcc gaggtaggca
     5641 aagagcgtgg caaagagcga tgagacaatg agcgcgagca gcggctcggc ggcgggccac
     5701 aggagcagga tcagcacgaa caaaccgtaa ttgacggctg caacactcca gcccaccacg
     5761 gcgtagcgca aaaattcagc aaggctcggc ggcacctgca gggcaaacgt gaaggtgcgg
     5821 tgggccaacc acccgaccag catggccagc gaaatggcaa acaggcgcgc gagcatggga
     5881 ttgaggccga cgagcacggt caaggccttg agggtgacgg cgtcggccat gaaggccaga
     5941 ccgcccgaga gcagaaaggc gaggccatgg cgcaggcggc tgcgtcgggc gccggtggta
     6001 gggctggcga acctctgcat ggcatcaaaa ccgaacgctc gcccagtcga ccggcggacc
     6061 accgggggga cgcgcgggcc gccagatgcg ctgcggtaag gcccggtcgc ccatcagggc
     6121 atcgtattcc atgcggcggc gggccgtcac cccgtcgccg tggccgggcc cggcgtcggg
     6181 atgtccagga tggcacatga cgatcggacg cgggccgagt ttttgcaggg cttgcgcgaa
     6241 ttcgctggcg taaggcactt tcgcgtcgaa gttggaaaaa ccggaaaatc ccgcattggt
     6301 gggcaggccg cggcgctggg ccgcggcgcc gaatccggcc gccagggtcg ccaccagcgc
     6361 cgccttgccc ttggcgatgc ggcgtgcggc aatggcctcc cagctgtcgc tggggtcccg
     6421 caccaggggg ggcagcgtcg gataccgccg ggccagcgta tcgagcacca cctcgcggat
     6481 gcccggcagc acgtggacgt ggtgatggcc gtcgacctga tcgggcgggc acgccagacc
     6541 gcgctcgaag tgatcgagct ggcgctcgaa ttcgtccctg atttcgcttc ggtccacgag
     6601 gccgagcagg cttgcgccga ccaatttggc gaggctcggc agcgtgcccg tcggtgccag
     6661 ccggggcatc cggcccagcg gagcccccag cgtcaagttg aaatgcagcc cgagcgccag
     6721 gtgcccgcgg tgcacggaga gccgcggggc cagccctggc caatgcgccg ccgtcaccaa
     6781 cacgctggtg gccgaaagcc ggcgcgccgc cgacagctcg ccgatcgcgc gggccacccc
     6841 cgagctcaac gcgtagtcat cggcgcacag aatgagcata ggctcgccca gtgatccttg
     6901 ccccctcggg ggcccttgcg gtcaagcctc gcccttggcg ttcgtgcggc gcggcgggcg
     6961 ggccgccttt tccagaccca actcttccgc caccagaaac aacggccggc ctttgacctc
     7021 ctcatagacc cggcccagat actcgccgat gatgccgagc gacaggagct gtaccccggc
     7081 aaaaaacatc accgagatca ccaaagtggg aaagcctggt acgtcggtgc catagatcaa
     7141 ggtcttgatc agaaagatca tggcatagca gaaggcgaac aaggaaatga gcaggccgac
     7201 gtaagaccag accttgagcg gtatggtcgt gaacgaggcg aggccgtcga tgccaaagcg
     7261 caacagctcg cgcggtcgcc aacggctttt gcccgccagt cgcgccggga cctggaaggg
     7321 cacgccgcgc gcgcgaaagc cgatccaggc atagagcccc ttggtgaagc gcgctctttc
     7381 tcccatgcta ttgatggcgt ccacggcctt gcggtcgagc aagcggaagt cgccggcgcc
     7441 ggcgggcagc acggtgcggg tcagtttgcg gaaggcggca tagtagacgc gcgcggccca
     7501 gcgatgggcg aggctgtcgg cggcgcgatc gaggcgctga ccgtagacga tgtcgtcgcc
     7561 ctgctgccaa tattgaacaa aggcggcgat cagttcgggc ggatgctgca ggtccgcgtc
     7621 catgacgatg acggcgtcgc cctgggcgta gcgtagcccg gccgccatgg cgctctcctt
     7681 gccgaagttg cggctgagca ccaggccctt gaggcgccgg tcgccggcgt tcagcgcctt
     7741 gagcttgtcg cgggtgttat cgctcgagcc gtcatcgacg aaaatgattt cccaatcggt
     7801 gccgagccct gccagcgccg gctgcaggcg cgccaacagg tcgtccaagc cggcggcctc
     7861 gttaaaggcc gggatcacca cgctgatgag ccctcgcgcc gggcgcgcct cgcgcggcaa
     7921 gggtgcggcg gcgtcgggat ccgggatcga caagaagcta tccttgacaa cgtcgaggcc
     7981 aggctcggct ttggccggac cgacgttccc attgccatca atgcgcgata atctcaagcg
     8041 ccgcgaggcc caacccggtc aggccccgcg ccgtcaatcg gtcatcacca caccggaatc
     8101 gatcaggcgg cggtggaagg ccaggatgtc ctcgcgccgg gcgcagctat agtagcgtc
//
LOCUS       DASTMT010000578         3105 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_5758103, whole genome shotgun sequence.
ACCESSION   DASTMT010000578 DASTMT010000000
VERSION     DASTMT010000578.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3105)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3105
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5758103"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1597)
                     /locus_tag="VFR00_13200"
     CDS             complement(<1..1597)
                     /locus_tag="VFR00_13200"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014496885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEU0160272.1"
                     /translation="MPDQTAPGRTPGPAFDAVIVGAGFAGMYMLHRLRCLGFSARVFE
                     AGGDVGGTWYWNRYPGARCDVESVQYSYQFDAALEQEWEWSERYAAQPELLRYARHVA
                     KRFDLNRDIQFNTRVSAARFDAAAGHWLIETSDGARTSAAFCIMATGCLSSPNLPSFK
                     GLERFSGERYHTGHWPHEDVDFRGKRVAVIGTGSSAVQSIPIIARQAEHVYVFQRTPN
                     YAVPAHNRPLAADELNRVKADYPAMRQRAKQTMTGIDFDYSDKKALKTSPQDRQHEYE
                     RRWRHGGLWFLGAYQDLMVEQAANDTAAEFVRGKIRERVTKAEVAELLSPKNTIGCKR
                     LCVDTGYYETFNRPNVTLVDVSSEPIEEITAGGLRAKGVDYEVDAIVFATGFDAMTGS
                     LLRIDICGRNDLTLKAKWQEGPRSYLGVAVADFPNLFMITGPGSPSVLTNMLPTIEQH
                     VEWIADALDFLRARGFALIEPSAEAEDDWVGHVAELAGRTLRYTCGSWYLGVNIMGKP
                     RVFMPYIGGFPSYVERCNEVAANG"
     gene            complement(1660..2406)
                     /locus_tag="VFR00_13205"
     CDS             complement(1660..2406)
                     /locus_tag="VFR00_13205"
                     /inference="COORDINATES: protein motif:HMM:NF036686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp/Glu racemase"
                     /protein_id="HEU0160273.1"
                     /translation="MPDVLGWRRKFGVLAPSTNTVVEPDFADLRPAGVTNHLSRIFTP
                     NVKGLSNETFRAGVATISDSVMDAIKSVMTCEPDYLVMGMSGITFLGGVKGADAFVRQ
                     VEEATGLAISIGSHAAAAALRAYGGVSRLAILSPYWPVMNEEVGRYFTEAGFQVVRDH
                     AMQVTSWVGIAAVSPAQCRDALARLDGDDVDAIVQVGTNLSMLRVAAAAELWLGKPVV
                     AINAATYWHALRSNDMREPIAGFGRLLQEF"
     gene            2530..>3105
                     /locus_tag="VFR00_13210"
     CDS             2530..>3105
                     /locus_tag="VFR00_13210"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU0160274.1"
                     /translation="MRNRKRVFYNSHGDEALYAMIRAAAGEGVELMTLERDDDGERKS
                     KLAEADAVIVAATRLSEDLLAAAPRLAFVHHQGVGYHDTVAWQVLAKRQIPLALTPGG
                     TAESVAEQTIMFILAVLRMLPYADSELRQGRFHVNAIRPVTRDLSGRTVGILGLGRIG
                     KAVARRLKPFDVKVIYHDILAMPPALDAELGV"
BASE COUNT          547 a         1030 c         1003 g          525 t
ORIGIN      
        1 agccgttggc cgcgacctcg ttgcagcgct ccacgtagct gggaaagcca ccgatatagg
       61 gcatgaacac gcgcggcttg cccataatgt tgaccccgag ataccaagag ccgcaggtgt
      121 agcgcagcgt gcggccggcg agctcggcca catgtcccac ccagtcgtcc tccgcctcgg
      181 cgctgggttc gatcagggca aaaccgcggg cacgcaggaa gtcgagcgcg tcggcgatcc
      241 actccacgtg ctgctcgatg gtcggcagca tattggtgag taccgacggg ctgcccggtc
      301 ccgtgatcat gaaaagattg gggaaatcgg cgacggcgac gcccagatag ctgcgcggcc
      361 cttcctgcca ttttgccttg agcgttaggt cgttgcggcc gcaaatatcg atccgcagca
      421 ggcttccggt catggcgtca aaaccggtgg cgaagacgat ggcatcgacc tcgtaatcga
      481 cacccttggc gcgtagaccg ccggcggtga tctcctcgat cggctcgctg ctgacgtcga
      541 ccagggtgac attggggcga ttgaacgtct cgtagtagcc ggtgtcgacg cacaagcgct
      601 tgcaaccgat ggtattcttc ggtgacagca gctcggccac ctcggccttg gtcacgcgtt
      661 cgcgaatctt gccgcgcacg aactcggccg ccgtgtcgtt ggccgcctgc tcgaccatca
      721 gatcctggta ggcgcccagg aaccacagcc cgccgtggcg ccagcgccgc tcgtactcat
      781 gctggcgatc ctgtggcgag gtcttgagtg ccttcttgtc gctatagtcg aagtcgatgc
      841 cggtcatggt ctgcttggcg cgctggcgca tggccggata gtcggctttg accctattga
      901 gctcgtcggc agcaagcggg cgattgtgcg ccggcacggc ataattcggc gtacgttgga
      961 aaacataaac gtgctcggcc tggcgcgcga tgatggggat agactgcacg gccgacgagc
     1021 cggtgccaat gacggcgacg cgcttgcccc tgaaatccac gtcctcgtgc ggccaatgac
     1081 cggtgtggta gcgctcgccc gaaaagcgct cgagaccttt gaacgatggc agattgggcg
     1141 aggacaagca gcccgttgcc atgatgcaaa aggcggcgct ggtgcgggcg ccatcgctgg
     1201 tttcgatcag ccaatggccg gccgcggcat caaaccgcgc ggcgctgacc ctggtgttga
     1261 actgaatgtc gcgattaagg tcgaagcgct tcgccacgtg cctggcgtag cgcaagagtt
     1321 ccggctgggc ggcgtagcgc tcactccact cccattcctg ctcgagggca gcgtcgaact
     1381 gataggagta ctgcacgctt tccacgtcgc agcgcgcgcc cggatagcgg ttccagtacc
     1441 aggtgccacc gacatcgcca ccggcctcga acacgcgcgc actgaacccc aggcagcgca
     1501 ggcggtgcag catgtacatg ccggcgaagc ccgccccgac gatcaccgca tcaaatgctg
     1561 gacccggcgt acggccgggg gctgtctgat ctggcatgat aaaacgtctt ttcccctgcg
     1621 tctgttaaca gcgccgcccg gccgggcccg tgcccgcccc taaaactcct gcagcagccg
     1681 gccaaaaccc gcgatcggct cgcgcatatc attgctgcgt agcgcatgcc aataggtcgc
     1741 cgcattgatg gcgacgacgg gcttgcccag ccacagctcg gcggcggccg ccacgcgtag
     1801 catcgacaga ttggtgccga cctgaacgat cgcgtcgacg tcgtcgccat cgaggcgggc
     1861 aagcgcgtcg cggcattgtg ccggggacac cgcggcaatg cccacccaag acgtcacttg
     1921 catggcatga tcgcgcacca cctggaagcc cgcctccgtg aagtagcgcc ccacctcctc
     1981 gttcatcacc ggccagtagg gcgacaggat cgccagccgc gacacgccgc cgtaggccct
     2041 gagcgccgcg gcggcggcat ggctgccgat cgaaattgcg agccccgtcg cctcctcgac
     2101 ctggcgcacg aaggcatccg cgcccttgac gccgccgagg aaggtgatgc cggacatgcc
     2161 catcaccaag tagtcgggct cgcaggtcat cacgctcttg atggcgtcca tcacgctgtc
     2221 agagatggtg gcgacgccgg cgcgaaacgt ctcgttgctg agccccttga cgttgggcgt
     2281 gaaaatgcgg ctcaagtggt tggtgacgcc ggccgggcgc aggtcagcga agtcgggctc
     2341 aacgacggtg ttcgtcgagg gcgccaagac gccgaacttt cgccgccagc ccaatacgtc
     2401 tggcatcagt tgtctccctt ggctaagcgt gtaggttgcc taagccatcg ctggcaccgg
     2461 cgatgcctgg cagcctagcc gagatcggcg ggccggccta cgggcgatcg agcgagggaa
     2521 aaacggccga tgcgcaatcg caagcgtgta ttctacaaca gccatggcga tgaggccctc
     2581 tatgccatga tccgcgccgc cgcgggcgag ggcgtcgagc tgatgacgct cgagcgcgac
     2641 gacgacggcg agcgcaagag caagctggcc gaggccgacg cggttatcgt cgccgccacg
     2701 cggctcagcg aggatttgct ggccgccgcc cctcggctcg cctttgtgca tcaccagggc
     2761 gtcggctacc acgacaccgt ggcctggcag gtgctggcaa aacgccagat ccccctggcg
     2821 ctcacgccgg ggggcacggc ggagagcgtc gccgagcaga ccatcatgtt catcctggcg
     2881 gtgctgcgca tgctgcccta tgccgacagc gagttgcgcc agggccgctt ccacgtcaac
     2941 gccatccggc ccgtcacccg cgatctgagc ggccgcacgg tcggcatctt gggtctcggc
     3001 cgcatcggca aggcggtggc ccggcgcctc aagcccttcg acgtcaaggt catctaccac
     3061 gacatcctgg ccatgccgcc ggcgctggat gccgagctcg gcgtc
//
LOCUS       DASTMT010000579         1876 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_5123812, whole genome shotgun sequence.
ACCESSION   DASTMT010000579 DASTMT010000000
VERSION     DASTMT010000579.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 1876)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1876)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1876
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5123812"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            513..1097
                     /locus_tag="VFR00_13215"
     CDS             513..1097
                     /locus_tag="VFR00_13215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0160275.1"
                     /translation="MNATQTSRAALKQSRAALRTIRLAVDTVADSIRQLQRLQGHAHR
                     GHRAALATVRMARHHSLNGGDKTRVEETVGKFARLMDWPLAMRSRSRVEPLERPLSDA
                     ESRLLANLAGSALLVAGGMLDITEQLDAWYGGLVVTAAAAARSALPSVTSQQHRERAD
                     KLMLRLEELCANAHIAWAFAVGQEAPPPPLRTLH"
     gene            1286..>1876
                     /locus_tag="VFR00_13220"
     CDS             1286..>1876
                     /locus_tag="VFR00_13220"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEU0160276.1"
                     /translation="MRQRPLARLALTCAMLANLAALPALAAERAAVQAACWQASALQW
                     QPGEELVVRASRHVRIPDIAGLGAERPPRLTPGTVRRVLLPPDKKLIALTFDLCETSG
                     ETAGYDGSIVDYLRSHAIKATFFAGGHWMASHKARTQQLLSDPLFELGTHGWAHRNTR
                     LLSGPDLQSEILAPSALYAAVRTELSQAQCAAPLRSA"
BASE COUNT          316 a          684 c          589 g          287 t
ORIGIN      
        1 aacggcgccc cggccacccc tcccctaatc ggcagctcga atgcgcggcg cctatcgcgt
       61 cccgccgcca aaagggcgct ttttgcgggc tcggccagcc cgcaggatct cccccaatcc
      121 ggtgatctct acccggctcg gcgatcccac ttcatgctcg tgcagtcgtt ggggatccca
      181 cgtcaatgga cctctttagc ttggtatccg tactgatcat gggcggggcg acgctttgcg
      241 gtctctccct cctcctctgg cttatcgtca tcgagcggaa agatgacggt tgcgacagcg
      301 agcatccccc ggcgcgggcc gctgcccgcc agaaagagcg gggcgcaagc ccccagcaaa
      361 cgcgcctacg ccggcctctg accccgcgcc aagccacccg cgcccctggc tcattgcaga
      421 cgcgctcgcg cccgcgcggt tcgggccgcg cctgaactat cgccaccccc tatcgcgcct
      481 ttcaccgtcg ccaagtcgga gctgaagctg caatgaacgc gacacaaacg agccgagcag
      541 ccctcaaaca aagccgcgcg gcactgcgga caatccgact cgccgtcgac acggtcgctg
      601 acagcatccg ccaattgcag cggctgcagg gtcatgcgca ccggggccat cgcgccgctc
      661 tggcgaccgt gcgcatggcg cgccaccact cgctcaatgg cggcgacaag acccgcgttg
      721 aggagaccgt cgggaaattc gcgcgcttga tggactggcc gctggccatg cgatcgcgca
      781 gccgcgtcga acctctcgaa cgcccactca gcgacgcaga gagccgcctg ctcgccaatt
      841 tggcaggctc tgccctgctc gtcgcgggcg ggatgctcga catcaccgag caactcgatg
      901 cctggtatgg agggctggtg gtgacggcgg cagcggcggc gcgctcggcg ctccccagcg
      961 taacgagcca gcagcaccgc gagcgcgccg acaagctcat gttgcggctg gaggagcttt
     1021 gcgccaacgc acacatcgcc tgggcgttcg ctgtgggcca agaggctccc ccaccgccgc
     1081 tgcgcacgct acactgagca cgcgcacgtg ctgaaacgac ggttacaccc ggccccgccg
     1141 cgccgtggcc gcaattgggg tgtttcaggg gccttagccg ctgacggcaa attggcaatc
     1201 gccggctgca ccgtttgggg aaaacgtgac cagcgaaggc cacagcacgc tggacccggc
     1261 gcgatggatc cgctattgcg ccgcgatgcg acaacgcccg ctcgcccgcc tagccctcac
     1321 ttgcgccatg ctggccaatc ttgcggcact gcccgccctg gcggccgaac gtgcggccgt
     1381 gcaagcggca tgctggcaag catcggccct gcagtggcag cctggcgagg aactggtggt
     1441 gcgcgcaagc cggcatgtgc gcattccaga cattgccggg ctcggcgcag aacgaccgcc
     1501 gcggctgacg cccggcaccg tccggcgcgt gctgctgccg cccgacaaga agctgatcgc
     1561 cctgaccttt gacctgtgcg aaacctccgg cgagaccgcc ggctatgacg ggtcgattgt
     1621 ggactacctg aggtcccatg cgatcaaggc cacctttttc gccggtggcc attggatggc
     1681 atcgcacaag gcgcgcaccc agcagctctt gagcgacccg ctgttcgagc tcgggacgca
     1741 cggctgggcg catcgcaaca cccgcctcct gagcgggccg gacttacaaa gcgaaattct
     1801 ggcgccgtcg gcgctctacg cggccgtccg cacggaactc agccaagcgc agtgcgcggc
     1861 gcctctcagg tcggca
//
LOCUS       DASTMT010000580         1659 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_5124729, whole genome shotgun sequence.
ACCESSION   DASTMT010000580 DASTMT010000000
VERSION     DASTMT010000580.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 1659)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1659)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1659
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5124729"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1347)
                     /locus_tag="VFR00_13225"
     CDS             complement(<1..1347)
                     /locus_tag="VFR00_13225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0160277.1"
                     /translation="MKRTGGQGAKRVVETTTRPTTRTLSRRPPAAVQPWRGGLLLLAK
                     ALVITSLVLASAGGLFYARLLHGPVPLGFLVEPLQRAIAEELAGFGVEIDNVAARLND
                     RHQLEFELSNVRIADSSNAPLAVAPSATFSISHRALLQGRIAPESVDLIAPRLLLYLS
                     EDGKVSLQFSAPTEAVELDRHKGAGSESPSKAMASANAAGLSDGSLGRIDLVKMLSEA
                     SARARRREYATAYLREVGLRSATLIVDNGSRKSIWRLPELDIDLDHRRSRSSIAGRAK
                     VESLAGPWSLNFRTFEAEKAGTLQLAVSVQNLVPRGLARTLPHLAALEGFDLPVWGEA
                     YLDLSSDGDILGGRIGIDAAPGTVALPWLADTAFAIDGGHLEISYQSGARRFEIAPSV
                     LVWGDSRVQFAGSVSHDARAPEGPAWAFALKSEGGWIGAELPAHERLPIDDWSAHGF"
BASE COUNT          282 a          586 c          538 g          253 t
ORIGIN      
        1 aaagccgtgc gccgaccaat cgtcgatcgg caggcgctcg tgcgcgggca gctccgcacc
       61 gatccagccg ccctcggatt tgagcgcgaa ggcccaggcg ggtccttccg gggcgcgcgc
      121 gtcgtgggaa acgctgccgg caaattgcac gcggctgtcg ccccagacca gcaccgaggg
      181 ggcgatctcg aagcgacgcg cgccgctctg gtaggagatt tccaaatgcc cgccatcaat
      241 ggcaaaggcc gtgtccgcca gccagggcag cgccaccgtt cctggcgccg cgtcgatgcc
      301 gatcctgccc cctaagatgt cgccatcact ggaaaggtcg aggtaggcct cgccccacac
      361 cggcaaatcg aaaccctcaa gcgcggccag gtgcggcaag gtgcgcgcca agccacgcgg
      421 caccaggttc tgcaccgaga cggcaagctg cagggttccg gccttctcag cctcgaaggt
      481 gcggaagttg aggctccacg gcccggccag gctttcgacc ttggcgcggc cggcgatcga
      541 actgcggctg cggcgatggt cgagatcgat atcgagttcc ggcagacgcc aaatgctctt
      601 gcggctgccg ttgtcgacga tcagggtcgc cgagcgcagc ccgacctcgc gcaaataggc
      661 cgtcgcgtat tcgcgccggc gcgcccgcgc cgaagcctcc gacagcatct tgacgagatc
      721 gatgcggccg aggctgccgt cgctgagccc tgcggcgttg gccgaggcca tcgctttgct
      781 ggggctctcg ctgcctgccc ctttgtgcct gtcgagctcg acggcctcgg tcggggcaga
      841 aaactgcagc gacaccttgc cgtcctcgct cagatagagc agcagccgcg gcgcgatcag
      901 atcgacgctt tcgggcgcga tgcgaccctg cagcagcgcc cggtggctga tggaaaacgt
      961 cgccgaaggc gccaccgcca agggagcatt gctgctgtcg gcgatgcgca cgttgctgag
     1021 ttcgaattcc agctgatggc gatcgttgag ccgggccgcg acgttgtcga tctcaacgcc
     1081 gaaacccgcc agctcctcgg cgattgcccg ctgcagcggc tcgaccagaa atcccaacgg
     1141 caccggtccg tgcaacaggc gcgcgtagaa caggccgcca gccgaggcca gcacgaggga
     1201 ggtgatcacg agcgccttgg ccagcaacag gaggccgccg cgccagggct gcaccgccgc
     1261 cggcggtcgc ctgctcaacg tgcgtgtggt tgggcgggtc gtggtttcca ccacccgctt
     1321 ggccccttgc ccgcccgtcc tcttcacgac gctccctcaa tcatgtggcg ccgagctgtc
     1381 gccctaggag cgacaagcaa agaactggcc gcaatcagcg gcgcgtcata cgcaacccca
     1441 cgcttgatac caacaaagcg aggcaaaaag gacgaaagga aggcgccttc ggcggcgtgg
     1501 ccccaattcg ggtcgcgccg ccacccgttt aagtttgcgg ctgcggttcc aatcccccgg
     1561 gacccggctc cggccgcgac ggacgcatct tgccggccgc cggcaccgcc ggcccagccg
     1621 gccctttcga ctcctcgtcg tcgtttgcac gcacgatct
//
LOCUS       DASTMT010000581         1716 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_6110965, whole genome shotgun sequence.
ACCESSION   DASTMT010000581 DASTMT010000000
VERSION     DASTMT010000581.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 1716)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1716)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1716
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6110965"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(288..>1716)
                     /locus_tag="VFR00_13230"
     CDS             complement(288..>1716)
                     /locus_tag="VFR00_13230"
                     /inference="COORDINATES: protein motif:HMM:NF017021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HEU0160278.1"
                     /translation="FDGKISPARALLGERGRMALAVTSPLFETSYDGTLLLGQEVALE
                     GKGRLKAASLQALAQWLERRTIADAGAVELAAALTLDKGRLVIADLAGVAGGSTLSGN
                     LALETERARPYLSGSVRASELDLGRLLLRGTASPRASDAKPAAAAEVESAPAGPAPAA
                     SPAKRRGHGGAWSEAPIDFAILRWGDADLSLVVDRVLYRELETGQSELGLSLANNVAK
                     LTLKDSLLYGGRGRGVLTFDASASEPAASGDLALDNVSYRPLLKDALGLEWLTGRGKM
                     TLSLSGKGESERKIIETLKGKAELAIGSGEFSGIDVGKLLRAIEQAQFHKLEVAPQDK
                     TPFSELAGTFQINNGVAETSDLRLIGPYMRLSGSGSSNLLERSADFSVRLKFAETAPS
                     EGAVIKVSGLEIPMHVSGPWERLAFKPDLKGIATSEQASEAIRQIGKNLNSPEVREAV
                     KGLLSGDGQQRPKAKELLEKLLKKQ"
BASE COUNT          285 a          553 c          567 g          311 t
ORIGIN      
        1 cgtcgtgcgc gcctagcgcg gtttgaccat gatcttcctt ttgggcggtg cttgaacggg
       61 tcaagtccat ccccaattgg aagagggaat cggtgtaggc cattggaccc ctccccaacc
      121 agcagataga taacccgctg cctccaattc ccaggggagt tggaagcaaa cggaacgcgt
      181 gatatggggc gaatcacccc tgcacgcaag cagaaatgtc ggcgccgggg aatttttctg
      241 caatcgtgtt tagcttcgcc gcgtccgctg tttagacgcg caaagctcta ttgcttcttc
      301 agcaattttt ccaagagctc tttggctttc gggcgctgct ggccatcgcc acttagaagc
      361 cccttcacgg cctcgcgcac ttcgggcgag ttgaggttct tgccgatctg acggatcgct
      421 tcgctggcct gctcggatgt ggcgatcccc ttaaggtcgg gcttgaaggc gagcctctcc
      481 caggggccgg acacatgcat gggaatttcc aggcccgaca ccttgatgac ggcgccctcg
      541 ctcggggccg tctcggcaaa cttgaggcgc acggaaaagt cggcgctgcg ctcgaggagg
      601 ttgctgctgc cgctgccgct caggcgcata tagggcccga tcaggcgcag gtcgctggtt
      661 tcggccacgc cgttgttgat ctggaaggtg ccggccagct cgctaaaggg cgtcttgtcc
      721 tggggcgcca cctccagctt atgaaactgg gcttgctcga tggcgcgcag caacttgccc
      781 acgtcgatac cggagaattc gccactgcca atcgcgagct cggccttgcc tttcagcgtc
      841 tcgatgatct tgcgctcgct ctcgcccttg cccgacaaac tcagggtcat tttgccgcgc
      901 ccggttagcc actcgaggcc aagggcatct ttcaagagcg gccggtagga gacattgtcg
      961 agcgcaagat cgccgctggc ggcaggctcg gacgccgagg catcgaaggt caaaacgccg
     1021 cgcccgcggc cgccgtaaag caagctgtct ttcagcgtca acttggcgac gttgttggcc
     1081 aggctgaggc cgagctcgct ttgcccggtc tcgagctcgc gatagagcac gcgatcaacg
     1141 accaggctga ggtcggcatc accccagcgc aaaatggcaa aatcgatggg cgcctcgctc
     1201 caggcaccgc catggccgcg ccgcttggcg gggctcgccg cgggggcggg cccggcgggg
     1261 gccgactcga cttcggcggc ggcggccggc ttggcgtcgg aggcgcgcgg ggacgcggtg
     1321 ccgcgcagca aaagccggcc aagatcgagt tcgctggcgc gcacgctgcc gctgaggtag
     1381 gggcgggcgc gctcggtctc gagcgccagg ttgccggaca gcgtgctgcc gccggcaaca
     1441 ccggcaaggt ccgcaatcac cagccgcccc ttgtccaggg tgagcgccgc ggcaagctcg
     1501 accgcgcccg catcggcgat ggtgcggcgc tcgagccatt gggcgagcgc ctgcagcgag
     1561 gctgccttca ggcgcccctt gccttccaat gcgacctctt ggccaagcag cagcgtccca
     1621 tcgtaggagg tctcgaacag aggcgaggtg accgccagcg ccatgcgccc gcgctcgccc
     1681 aggagtgcgc gggcggggga gattttgccg tcgaag
//
LOCUS       DASTMT010000582         2484 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_4468915, whole genome shotgun sequence.
ACCESSION   DASTMT010000582 DASTMT010000000
VERSION     DASTMT010000582.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2484)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2484)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2484
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4468915"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..845)
                     /locus_tag="VFR00_13235"
                     /pseudo
     CDS             complement(1..845)
                     /locus_tag="VFR00_13235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015687616.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            complement(842..1834)
                     /locus_tag="VFR00_13240"
     CDS             complement(842..1834)
                     /locus_tag="VFR00_13240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015687615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0160279.1"
                     /translation="MIAAPLLTVKDLRVAFEGDRGRLTQAVNGTSFALAGGRTLGIVG
                     ESGCGKSVTALAIMRLLTAAGTRISGSITFEGRELVGLPERQMRDLRGNRLAMIFQEP
                     MTSLNPSFTVGDQVAEVLIRHRGLAAAAARALAIDMLARVRIPSPAARAEEYPHKLSG
                     GMRQRVMIAMALACAPALLIADEPTTALDVTIQAQILDLIGKLREETGAAIILITHDL
                     GVVAETCDDVLVMYTGEIVEQAPTRQLFLRPQHPYTVGLLGSLPRLGEKRERLSAIAG
                     SVPSLMGIEFVGCRFANRCPFADAHCRAEAPPLRELSSGHWSRCWKAPLDRLAA"
     gene            complement(1831..>2484)
                     /locus_tag="VFR00_13245"
     CDS             complement(1831..>2484)
                     /locus_tag="VFR00_13245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007593858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1028 domain-containing protein"
                     /protein_id="HEU0160280.1"
                     /translation="TKFFAVGAVVPHIKTGVGAVASQAFINPYYGAQGLELLAAGLRS
                     EAVLAKLVAGDEGRANRQLHLVDGRGNFAAYTGAACIDWCGHELRAGYSVAGNMLAGP
                     AVLRETINSYEANAQLPFARRLLGAMQAGEAAGGDKRGKQSAALLIHDGEDYPLYDLR
                     VDDHPEPLAELARLEEIARQRWLHFRRCMPNRRQTSGLVDRAQLEELIATSIAEGYQ"
BASE COUNT          445 a          844 c          832 g          363 t
ORIGIN      
        1 gtgtgcatcc ggctcggcga tgagggcggg tgactccccg gcgcaacgcc gagcggcaaa
       61 ctgacagcgc ggatgcaaat ggcacccgct cggcggcgca atgggactgg gcggatcgcc
      121 cggcagcagc tggcgctctc gacgatgcgg ccgaggtaca tcaccgccac ccggctggcg
      181 atatgcttga ccaccgccag atcgtgactg atgaagatga aggcgagccc cagccgtgcc
      241 tgcaggtctt tgagcagatt tagaatttgg ctgcggatcg aaacgtcgag ggccgagacg
      301 ggctcatcgc agacgatcaa cttgggctcg agggccaagg cgcgcgcaat ggcgatgcgc
      361 tgacgctggc cgcccgagaa ctcgtgcgga aagcgctggc cgtgctgggg gatgagccca
      421 acggtacgca ggagctcacc gacgcgtgcg cggcggtccg ccggcgtgag cgttgtgtgc
      481 aacaagagcg gctcggccag aatctcgccc acgcgcatgc ggggattgag cgaactgtag
      541 ggatcctgaa agatcagctg ggcacccgcg cgcgcccgtc gcagctgggc ccgcgagagg
      601 gacgcaagat cctgaccatc gaagcgaacg cttcccgcac tgggctcgat cagccgcagc
      661 gccagacggc cgaccgtgga tttgccgcaa cccgattccc caaccagtgc cagggtctca
      721 ccgcgatcga cgctgagcga gacgccatcg acggcgcgca cgagggccgt cggcacgccg
      781 aacagcgagc gtcgcaccac gaaggacttg gcgacgtcgt cgagcacgag cagcggcgag
      841 gtcatgcggc aagcctatcc agtggcgcct tccagcagcg cgaccaatgc ccgctgctga
      901 gctcgcgcag cggcggggcc tcagcccggc agtgcgcgtc ggcgaaggga cagcggttgg
      961 caaagcggca gccgacgaac tcgatcccca tcaggctcgg cacgctgccg gcgatggccg
     1021 ataggcgctc gcgcttctcg ccaagacgcg gcagcgaccc gagcaagccc accgtgtagg
     1081 gatgctgcgg gcgaaggaac agctgccggg tcggtgcctg ctcgacgatc tcgccggtat
     1141 acatgaccag cacatcatcg caggtctccg ctaccacccc aaggtcgtgg gtgatcagga
     1201 tgatggccgc cccggtctct tcgcgcagct tgccgatgag atcgaggatt tgcgcctgga
     1261 tagtgacgtc gagggccgtc gttggttcat cggcgatcag caaggccggc gcacacgcca
     1321 acgccatggc gatcatcacg cgctgacgca tgccgccgga aagcttgtgc ggatactcct
     1381 cggcgcgcgc ggccggtgag ggaattcgaa cgcgcgccag catgtcgatg gcgagcgcgc
     1441 gcgccgcggc agccgccagg ccgcgatggc gaatgaggac ctcggcgacc tgatcgccaa
     1501 ccgtgaaact cgggttaagc gaggtcatcg gctcctgaaa gatcatggcc agccgattgc
     1561 ccctgagatc tcgcatctgc cgctcgggta ggcccacgag ctcgcggccc tcaaacgtga
     1621 tcgacccgga aatgcgcgtg ccggccgcgg tcagcaaccg cataatggcg agtgccgtca
     1681 cgctcttgcc gcagccggat tcgcccacga tccccagcgt gcgcccccct gccagcgcga
     1741 agctggtgcc gttcacggct tgcgtgagcc tgccgcgatc gccctcgaag gcgacccgca
     1801 aatccttgac cgtgagcagc ggcgccgcaa tcactggtag ccctcggcaa tcgaggtggc
     1861 gatgagctct tccagctgcg cgcggtcgac caggcccgag gtttgccgcc gattgggcat
     1921 gcagcgccgg aaatgcagcc agcgctggcg cgcaatctcc tccaatctcg ccagctccgc
     1981 caagggctcg gggtggtcgt ccacgcgcag gtcgtagagg ggataatcct ccccgtcatg
     2041 gatcaggagg gccgccgact gcttgccgcg cttgtcgccg ccggccgcct cacccgcctg
     2101 catggcgccg agcaggcgcc gggcgaacgg cagctgcgca ttggcctcgt agctgttaat
     2161 ggtttccctc agcaccgccg ggcccgccag catattgccg gcaacgctgt agcccgcgcg
     2221 cagctcatgc ccacaccaat cgatgcaggc cgctcccgtg taggcggcaa agttgccgcg
     2281 gccgtcgacg agatgcagct gacgattggc ccggccctcg tcccccgcaa cgagcttggc
     2341 gagcaccgcc tcgctcctca agcctgcggc caagagctca aggccctgcg cgccgtagta
     2401 gggattgatg aaggcctgcg aggcaaccgc ccccaccccg gttttgatat gcggcaccac
     2461 cgccccgacg gcgaagaatt tggt
//
LOCUS       DASTMT010000583         4422 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_5128324, whole genome shotgun sequence.
ACCESSION   DASTMT010000583 DASTMT010000000
VERSION     DASTMT010000583.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4422)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4422)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4422
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5128324"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..244
                     /locus_tag="VFR00_13250"
     CDS             <1..244
                     /locus_tag="VFR00_13250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013950018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEU0160281.1"
                     /translation="LAQELKELAQTAGLDPRCVSPHVLRHAFASHLLDRGADLRSVQQ
                     LLGHVDISTTQIYTHVLEERLKKLVFEHHPLAPSRH"
     gene            380..1354
                     /locus_tag="VFR00_13255"
     CDS             380..1354
                     /locus_tag="VFR00_13255"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011439433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HEU0160282.1"
                     /translation="MDRPLTVRTYLDFEKPIAELESKVAELSALSVEDDGSVSITEEV
                     TKLQQKAHQALVDTYAKLTPWQKTQVARHPDRPHCLDYVEALIEDFTPLAGDRYYAED
                     AAIMGGIGRFRGRSVMLIGHEKGSDTEGRIKHNFGMARPEGYRKAVRLMDLAERFGMP
                     VITLVDTAGAYPGIGAEERGQAEAIARSTDRCLSLGVPLVAVIIGEGGSGGAVAIATA
                     NRVLMLEHAIYTVASPEAAASILWRDPARAIDAATSMKITAQDLKQLGVIDVIITEPV
                     GGAHRDTAMIFKQTAGAIAGALTEFSGMSADGIREQRQEKFLAIGRNL"
     gene            1508..2464
                     /locus_tag="VFR00_13260"
     CDS             1508..2464
                     /locus_tag="VFR00_13260"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase family protein"
                     /protein_id="HEU0160283.1"
                     /translation="MSLIISGANAKRAVAMLLACCCLVTATLGGCMKVELVGPHLQPL
                     SQDTLMLLGKKGMDQQAPIFIRIFKEESELEVWKLKNDGRFYHFKTYPICNWSGELGP
                     KLKEGDRQAPEGFYTISREQMNPDSKYHLAMNLGYPNAYDKAHGRTGEFLMIHGNCKS
                     AGCYAMTDALMEEIYAIARDSFLGGSDAFEVYAFPFRMTEANMARQAKHAAYPFWLTL
                     KEAYDYFELTRQLPAVAVCNRRYVVNVAMRGDLKQLNPTATCPPFRRPKPVPFRPTND
                     EAAAEGAGKSRAPAGAEPQALRSGLIRTPLSAIPSLSVAPVR"
     gene            2613..2843
                     /locus_tag="VFR00_13265"
     CDS             2613..2843
                     /locus_tag="VFR00_13265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0160284.1"
                     /translation="MKRNLLLSLAGASAAIVIGLATAQAAPSSSVGDAVRSYAQDQSA
                     VQDVRWHRHCYNRCHWHHGRRHCRRICHRRWW"
     gene            complement(2901..3236)
                     /locus_tag="VFR00_13270"
     CDS             complement(2901..3236)
                     /locus_tag="VFR00_13270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0160285.1"
                     /translation="MLSPPTPLAPHEHARVWGWQRRMFVFYGIAIALLAAAGAAMFGF
                     GELAWVRRAALGVLAAIVALATAVQFSERCPRCGLRLGAHGGLLLPEQCRGCGVAFHP
                     SPADNAGDD"
     gene            complement(3230..4120)
                     /locus_tag="VFR00_13275"
     CDS             complement(3230..4120)
                     /locus_tag="VFR00_13275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017487231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEU0160286.1"
                     /translation="MPEAQFPDPRRNAYRDDLAAEGLRGKVKAPAYSPGWDRQIVFPA
                     TPLRARPDARANWTTEALFGELVKVYDEAGEWAWVQLAADGYVGYVRRSALAPQGASV
                     THRVSALGTFLYAAADMKAAPLMPLSMNALLSIVEVGPSFARLVDGTFVPSRHIVESQ
                     RFVADYVAAAERFVGVPYLWGGKTRFGLDCSGLVQTALAAAGRLCPRDSDMQEAELGQ
                     SLEGGDRLDALERGDLVFWQGHVGIMADGFMLLHANAHHMAVVVEPVRSAVERIARAG
                     LPISSVQRLRKRSQTAAVAC"
     gene            complement(4120..>4422)
                     /locus_tag="VFR00_13280"
     CDS             complement(4120..>4422)
                     /locus_tag="VFR00_13280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase family protein"
                     /protein_id="HEU0160287.1"
                     /translation="AEAGLRVGDPVWRLPFWDGYEASLDSPVADLSNVSEGAFAGSVT
                     AALFLRRFVRQTRRFAHFDIFGWRPAPRALGPKGGETQVARAMFELLRGDFAGRTT"
BASE COUNT          783 a         1513 c         1406 g          720 t
ORIGIN      
        1 cctcgcccag gagctcaagg aattggcaca gacggcaggg ctcgatccac gctgcgtctc
       61 gccgcacgtg ttgcggcacg cctttgccag ccacctgctc gaccgcggtg ccgacctgcg
      121 ctccgtgcag cagctcttgg gccacgtcga tatttcgacc acgcagatct acacgcacgt
      181 gctcgaggag cgcctgaaga agctcgtttt cgagcaccat ccattggcgc cgtcccgcca
      241 ctaggcccgc cgttagcgcc gcccgccggg cagctttccg cgccgccggc ccgggcccgt
      301 taacttgact tgaggcgctc gcgcggccta agtcgccctc gagcctgccc gcgtgacagc
      361 gggccatttg gtcgtcgcca tggatcgtcc actcaccgtt cgcacctacc tcgattttga
      421 gaaaccgatc gccgagctcg agagcaaggt ggccgagctc agcgcgctca gcgtggagga
      481 cgacggttcc gtctcgatca ccgaagaggt gacgaagctg cagcaaaagg cgcaccaggc
      541 gctggtcgat acctacgcca agctcacgcc ctggcagaag acacaggtcg cgcgccatcc
      601 ggaccggccg cattgcctcg attatgtcga ggccctgatc gaagatttca cgccgctcgc
      661 gggcgaccgt tactacgccg aagacgccgc catcatgggg ggcatcggtc gctttcgcgg
      721 tcgctccgtg atgctgatcg gccacgagaa gggctccgac acggagggcc gcatcaagca
      781 caacttcggc atggcgcgtc ccgaaggtta ccgcaaggcc gtgcgcctga tggacctcgc
      841 cgagcgcttc ggcatgccag tcatcacgct ggtcgatacg gcgggcgcct atcccggcat
      901 cggcgcggag gagcgcggcc aggccgaggc cattgcccgc tcgaccgatc gctgcttgtc
      961 gctcggcgtg ccgctcgtgg ccgtcatcat cggcgagggt gggtcagggg gtgccgttgc
     1021 catcgccacc gccaaccggg tcttgatgct cgagcacgcc atttacaccg tggcctcgcc
     1081 ggaggccgcc gcctccattc tttggcgcga tcccgcgcgc gccatcgatg ccgccaccag
     1141 catgaaaatc acggcgcagg acctgaaaca attgggggtg atcgacgtca ttattaccga
     1201 accggtgggc ggcgctcatc gcgatacggc catgatcttc aagcagaccg ccggggcgat
     1261 tgccggcgcc ctgacggagt tttctggcat gagcgcggat ggcattcgcg agcagcgcca
     1321 ggaaaaattc ttggcgatcg ggcgcaacct gtagcgcgcg ggccgtccgt cttttgtggc
     1381 gcatttgcgg cgcgccggat cggcagaccc gcgccagcgg cgccggtctg aggggttgtc
     1441 ttcacattaa ccccatatta acgtttgcga atcattggtg atcgccacac gcctggggca
     1501 cgcgcgtatg agtttgatca tctcgggggc caatgccaaa cgcgccgtgg cgatgctgct
     1561 ggcgtgctgt tgtctcgtga ccgccacgct gggtggttgc atgaaggtcg agctggtcgg
     1621 tccccacctt cagccgctgt cgcaagacac cttgatgctg ctcggcaaga agggcatgga
     1681 tcaacaggcc ccgatcttca tccgcatttt caaggaagaa tccgagctcg aagtctggaa
     1741 gctcaagaac gatggacgct tctatcattt caagacctac ccgatctgca attggtcggg
     1801 cgagcttgga cccaaactga aggagggtga ccggcaggcg ccggaagggt tctacacgat
     1861 cagccgcgag cagatgaacc ccgattccaa gtatcacctg gccatgaacc tcggctaccc
     1921 caacgcctat gataaggcgc acggccgcac cggtgaattc ttgatgatcc acggcaactg
     1981 caaatcggcg ggctgttacg ccatgaccga cgcattgatg gaggagattt acgccatcgc
     2041 gcgcgattcc ttcctcggcg gcagcgacgc gttcgaagtg tacgccttcc ccttccgcat
     2101 gacggaggcc aatatggcgc gccaggccaa gcacgcggcc tatccgttct ggttgacgct
     2161 gaaagaggcc tatgactact tcgagctgac gcggcagctc cccgccgtgg cggtttgcaa
     2221 tcgccgctac gtcgtcaacg tcgccatgcg cggcgatttg aaacagctca atccgaccgc
     2281 cacctgcccg ccgttccgcc gccccaagcc cgttccgttt cgcccgacca acgacgaggc
     2341 ggcggccgag ggcgccggca aaagccgtgc gcccgccggc gcggagcccc aggccttgcg
     2401 ctcggggctg atccgaaccc ccctcagcgc cattccaagc ttgagcgttg cgccggtccg
     2461 ctagcctcgg cgtggatgca aaaccccgct tcggccaagg tcattcacag tgcattcata
     2521 gaccgggctt gatgatcgcg cggtcgagca aatcaggccg atgccgggag caaggattgc
     2581 tctgggcggc aaccaactgg agcacggaac gcatgaaacg gaacctactg ctgtcccttg
     2641 caggcgcaag cgcggccatt gtcatcggcc tcgcaacggc gcaggccgcg ccttcctcca
     2701 gcgtgggcga tgccgtccgc agctacgccc aagaccagag cgcggtccag gacgtgcgct
     2761 ggcaccgtca ctgctacaac cgctgccact ggcaccacgg tcgtcggcat tgccgccgca
     2821 tttgtcatcg tcgctggtgg taacaaacac gacggcgcga caggccggcc ccagcctggg
     2881 ctggcctgtg gcgttttgct ctagtcgtcg cccgcgttgt cagcgggtga tgggtggaag
     2941 gccaccccgc agccgcggca ctgctcggga agcaacagcc ctccatgggc gccaaggcgc
     3001 agcccgcagc ggggacaacg ctcgctgaac tgcacggcgg tggcgagcgc gacaatggcg
     3061 gcgagcacgc cgagcgcggc gcgtcgcacc caggcgagtt cgccaaaacc gaacattgcc
     3121 gcccccgccg ccgccaacag cgcaatggcg atgccgtaga agacgaacat gcgacgttgc
     3181 cagccccaca cgcgggcgtg ttcgtgcggc gccagcggtg ttggcgggct cagcacgcca
     3241 ccgccgccgt ttgcgacctc ttgcgcagcc gttgcaccga gctaatcggc agtcctgcgc
     3301 gcgcaatgcg ttcgaccgcg cttcgcacgg gttccaccac gacggccata tggtgggcat
     3361 tggcatgcaa gagcatgaag ccgtcggcca taatgccgac gtggccctgc cagaacacga
     3421 ggtcaccacg ctcgagcgca tcgagacggt cgccaccctc caggctttga ccgagctccg
     3481 cctcctgcat gtcgctgtcg cgcgggcata ggcgccccgc agcggcaagc gcggtttgca
     3541 ccagccccga gcaatctagg ccgaagcgcg tcttgccgcc ccagagatag ggcaccccca
     3601 caaagcgctc ggcggcggcg acgtaatcgg cgacgaaacg ctggctctcc acaatgtgtc
     3661 ggctcggcac gaacgtgcca tcgacgagcc tcgcaaaact ggggccgact tcgacgatgc
     3721 tgaggagcgc gttcatgctg agcggcatca gcggggccgc cttcatgtcg gctgcggcat
     3781 aaaggaaggt gccgagcgcg ctgacgcggt gggtgacgct ggctccttgt ggcgcgagcg
     3841 ccgagcggcg cacgtagccc acgtacccgt ccgccgcgag ctggacccag gcccattcgc
     3901 ccgcttcgtc gtagactttg accagctccc cgaacagggc ctccgtggtc caattcgccc
     3961 gcgcgtccgg ccgtgccctg agcggggtcg ccggaaacac aatctggcga tcccagccgg
     4021 gcgagtaggc gggcgccttc accttgccgc gcaggccttc ggcggcgagg tcgtcgcggt
     4081 aggcgttgcg ccgcggatcc ggaaattgag cctcgggcat caggtcgtac gccccgcgaa
     4141 atcgccgcgc agcagttcga acatggcgcg tgccacctgc gtttccccgc ctttggggcc
     4201 gagcgcgcgc ggcgccggcc gccagccgaa gatgtcgaag tgcgcaaaac gccgcgtctg
     4261 ccgcacgaag cgacgcagaa acaatgccgc cgtgaccgag ccggcaaagg ccccctcgga
     4321 gacgttgctg aggtcggcca ccggactgtc gaggctcgct tcatagccgt cccagaacgg
     4381 caggcgccag accggatcgc cgacgcgaag accggcctcg gc
//
LOCUS       DASTMT010000584         5009 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_4470588, whole genome shotgun sequence.
ACCESSION   DASTMT010000584 DASTMT010000000
VERSION     DASTMT010000584.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 5009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5009
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4470588"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..884)
                     /locus_tag="VFR00_13285"
     CDS             complement(<1..884)
                     /locus_tag="VFR00_13285"
                     /inference="COORDINATES: protein motif:HMM:NF024868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU0160288.1"
                     /translation="MTKDQADLVSSSPLFRPIAVPQPCLMATVSTERTSPATAPSRCG
                     KIVLLITEDWFALSHFRPLISLLSELAREVVVVTRSSGRLPEIEALGARTLAFDYYRG
                     SNKQAQAAQVAWRLARLLRRERPDVVHLIAMKPIVLGGLALRWVRVPHVVVHLTGQGL
                     AGIATDPLLRLYRFAMLRVLASLVRKPTSRLLVENPDDLALIAKLVPRLGPRFAILGG
                     AGVDPAAFPALAAPPNDVPIAAHVGRMIRSKGIDLLMQAYDQLRREGARLQLEFFGSS
                     DRDNPEAIPPHAIETWCQG"
     gene            1203..2516
                     /locus_tag="VFR00_13290"
     CDS             1203..2516
                     /locus_tag="VFR00_13290"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEU0160289.1"
                     /translation="MTRLAGRQGDATPRVGVVVPWARLMAAGGAAIFAGLVVAIVLQP
                     HLTTVLLPALGLYAFAGLRGESRRWPFSWRAVWVLPSIFALYVALSATWGSDPRAALI
                     AGTLFGIFLVATRIAFIGALEAEPSALERLSGYFVAAVAVAALLMAITIYSHGALHKA
                     ICAVFPALAPTDRDFVRLDGNSLEFVGLPYFNRNIALLNLLLWPALLVLAKRVVDWRT
                     ALLPLGVLALVALATFGSEHQTSMLALLASGLIFLVYRLSPRSARILVVVGWVAALLL
                     VRPAVLTAYEAGLHQASWMQESARARIILWAYTARQMSKAPILGVGAQTTRADAEAAR
                     PPEVPGQAFPQWTGRHGHNIYLQTWYELGVIGAGLLLAMGLLILSWLGTLARPGQDYA
                     AAAFVCASIMCASSFGMWQAWFIAATMLVVPMLALGLQWYGDPPR"
     gene            2831..4246
                     /locus_tag="VFR00_13295"
     CDS             2831..4246
                     /locus_tag="VFR00_13295"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEU0160290.1"
                     /translation="MTAVGALKPSFASSKTTAAPTGLHVPRVAPLDGPLGAVAFFAKL
                     LHNPLRILPAAAYEEGIVWAERAGRRICWITEPGLIKTVLLDKRDVFERTAVTQRLLG
                     PLLGNGVLTADGADWKWQRQTAAPVFRHHDLLSFVPVIAEAAERLLGDWRQDAYGATR
                     NIDRDMTLVTFDVISHTLLPGGDAHVGPLIGRANVDYQKPLGWQMAYANFRLPAWLPH
                     PGWLKMQVARRQLRSAVASLVAERRAHPSGKDDLLQRLANAKNPETGAQMPDELLIDN
                     LLTFLMAGHETTAKALTWTLYLLARAPAWGDRLAEEVRRVVGDGPVQPEHIDKLVLTT
                     QVLKESMRLYPPAPIMSRQASADTELAGGPVKAGTQVIIPIYAIQRHRRRWSNPDHFE
                     PERFAPEHEAEIPRYQYMPFGAGPRICIGMAFAMIEGIAILASLVRAASFAVTPGHEP
                     EPISRVTLRPAGGMPLAVRLR"
     gene            complement(4441..4731)
                     /locus_tag="VFR00_13300"
     CDS             complement(4441..4731)
                     /locus_tag="VFR00_13300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0160291.1"
                     /translation="MNLTALIIQLIAGALGGNAAGNLAKDINLGPLGNSIAGAIGGGV
                     GGQVLNAVLGIAGTAAASGLDVGAIVSAFVTGGVGGGLTALIVGFLKAKLAN"
BASE COUNT          800 a         1625 c         1707 g          877 t
ORIGIN      
        1 ccctggcacc aggtctcgat ggcgtgcggc gggatggcct cggggttgtc gcgatcgctg
       61 ctgccgaaaa actcgagctg cagtcgggcc ccctcgcgcc gcagttgatc gtaggcctgc
      121 atcagcagat caatgccctt cgagcggatc atgcgcccga cgtgggcagc gatggggacg
      181 tcgttgggcg gcgccgccag cgccgggaag gctgcgggat cgacgccggc tccgcccagg
      241 atggcaaagc gcgggccgag ccggggtacg agcttggcga tcagggcgag gtcgtcgggg
      301 ttctcgacca gaagacgcga agttggtttg cggaccaggg aggccaacac ccgcagcatg
      361 gcgaaacgat aaagacgcaa cagcggatcg gtggcgatgc cggcgagacc ctgcccggtg
      421 agatgcacga cgacgtgcgg cacgcgcacc catctgagcg ccaggccgcc gagaacgatg
      481 ggcttcatgg cgatgaggtg cacgacgtcg gggcgctcgc gtctcagaag gcgtgccaac
      541 cgccaggcca cctgcgccgc ctgcgcctgc ttgttggagc cgcgatagta gtcaaacgcc
      601 aacgtgcgcg cgcccagcgc ctcgatttcg ggcaggcgac cggaggagcg cgtcaccacg
      661 accacctccc gggcgagctc gctcagcagc gaaatcagcg gccgaaaatg tgacagggca
      721 aaccagtcct cggtaatcag caaaacgatc ttgccgcagc ggctgggcgc tgttgcgggc
      781 gaggttcgct cggtggacac ggtggccatg agacagggtt ggggcacggc gattggacga
      841 aacaacgggg acgaactgac gaggtcggcc tgatcttttg tcacggcctc gcctctcgac
      901 catgctctgc cagcaaagga aagcagcccg gcaaggaaac atgtggagag ctcattgatg
      961 tatgtactag cgccggaatc aaggccatga tgcgccccga ccgcgcgggt gcggcgacgt
     1021 gcgcgcgccc ctaagcggtt tgcaacgggg acctgcggct ttttgcttgg tgtcattttc
     1081 ctgcgtcacg gtccggctag gaatgggcga cagggcgacg gattttgcgc aggttgcgcg
     1141 ttgcaaccgg ggcatttttt tgcccctcaa accgtgacct cgagcgcggg gacttggcga
     1201 gcatgaccag gctggcaggc aggcaggggg atgcgacccc acgcgtcggg gtggtggtgc
     1261 cttgggcgcg gctgatggcc gccggcggcg cggcgatttt tgccggcctc gtcgtcgcca
     1321 tcgtcttgca accgcatttg accaccgtgc tgttgccggc cctcgggctc tatgcgttcg
     1381 cgggcttgcg tggggaaagc cgccgctggc cgttttcctg gcgtgcggta tgggtgctgc
     1441 ccagcatctt tgcgctctac gtggcgctca gcgctacctg gggcagtgat ccccgtgcgg
     1501 ccctgatcgc gggcacgctg tttggcatct tcttggtggc aacgcgcatt gcctttatcg
     1561 gcgcgctcga ggcggagcca agcgcactcg agcggctcag tggctacttt gtcgccgcgg
     1621 tcgcagttgc cgccctgctg atggccatca ccatctattc gcacggcgcg ctgcacaagg
     1681 cgatttgcgc ggtcttcccg gcgctggccc ccaccgatcg cgacttcgtc cggctcgacg
     1741 gcaatagctt ggaattcgtg gggctgccct acttcaaccg caacatcgcg ctcctcaatc
     1801 tgcttttgtg gccggcgctg ctggtgctgg cgaaacgggt cgttgactgg cgcaccgcgc
     1861 tgctcccctt gggcgtgttg gcattggtgg cgctggcgac gtttggctcc gagcaccaga
     1921 cttccatgct ggcgctgctg gcctccggcc tgatctttct cgtctatcgc ctctcgcccc
     1981 gcagcgcgcg catcctggtc gtggttggtt gggtggcggc gttgcttctg gtccgccccg
     2041 cggtgttgac cgcctacgag gcgggcctgc atcaggcgag ctggatgcag gagagtgccc
     2101 gcgcgcgcat tattctgtgg gcgtacacgg cgcggcaaat gtccaaggcg cccatcctcg
     2161 gcgtcggtgc acaaaccacg cgcgcggatg ccgaggcggc ccgcccgccc gaagtgccgg
     2221 gccaagcctt tccgcaatgg acggggcggc acggccacaa catctatctg cagacctggt
     2281 acgagctcgg cgtcatcggt gccggtctgc tgctggcgat gggcctcttg atcctgagtt
     2341 ggctcgggac cttggcgcga ccgggccagg actatgcggc ggcggccttt gtttgcgcca
     2401 gcatcatgtg cgcttcgagc ttcggcatgt ggcaagcctg gttcatcgcc gccaccatgc
     2461 tggtggtgcc catgctcgcc ctgggtctcc agtggtacgg cgatccgccg cggtgagcac
     2521 gccttcgcgc cgagcgctac gccaattgaa gcgacgacgc tgagcgggac caggttccca
     2581 ggacattggg cgatccgcgt gctgccgttt gcgtggtgac ggcgcgcttt ggcgcgcagc
     2641 agcaacgtcg agctgatctc ttccccgggg caacggacct gtccgcgaca agatcggcgc
     2701 gcggtgccag gcctgctggc gagcctggcc gggcggcgtt tggctcgtgc ttgcggccgc
     2761 cgctctgtgc taggatgccg gccgctcagc acgctcgcgt gggacacgca ccgctcagga
     2821 gaaaacgagc atgaccgccg tcggggctct taagccatcg tttgcctcca gcaagaccac
     2881 tgcggcgccg acggggctgc atgtgccccg cgttgccccg ctcgatggtc cgctcggcgc
     2941 cgtcgcgttt ttcgccaagc tgctgcacaa tccgctgcgc atcctgcctg cggccgccta
     3001 cgaggagggg atcgtctggg ccgagcgcgc cggccgccgc atctgctgga tcaccgaacc
     3061 cggtctcatc aagaccgtcc tgctcgataa gcgcgatgtc ttcgagcgca cagccgtgac
     3121 gcagcgcctg ttgggtcccc tgctcggcaa cggcgtcttg accgcggacg gcgccgattg
     3181 gaagtggcag cgtcagacgg cggccccggt gtttcgccat cacgacctct tgagtttcgt
     3241 gcccgtgatc gccgaagccg ccgagcgcct gcttggggac tggcggcagg acgcctacgg
     3301 ggcgacgcgc aacatcgacc gcgacatgac gctcgtcacc ttcgacgtga tttcgcacac
     3361 cttgctgccc ggcggcgatg cgcacgtggg accactgatt ggtcgcgcca acgtcgacta
     3421 tcaaaagccg ctcggttggc aaatggccta tgccaatttc cgtcttccgg cctggctgcc
     3481 gcatccgggg tggctcaaga tgcaggtggc gcggcgccag ctgcgatcgg cggtggcctc
     3541 gctggtcgcc gaacggcggg cgcatccgag cggtaaagac gacctgttgc agcgcttggc
     3601 caatgccaag aacccggaaa ccggcgccca gatgccggac gagctgctga tcgacaatct
     3661 cctcaccttc ttgatggccg ggcacgagac cacggccaag gcgctgacct ggacgctcta
     3721 tcttttggcg cgcgcgcccg cctgggggga tcggctcgcc gaggaagtgc gaagggttgt
     3781 cggcgacggt ccggtgcagc ccgaacacat cgacaagctc gtgctcacca cgcaggtgtt
     3841 gaaggaaagc atgcgccttt atccgccggc gcccatcatg tcgcggcaag ccagcgccga
     3901 taccgaactt gctggcgggc cggtcaaggc cggcacgcag gtgatcattc ccatctatgc
     3961 cattcagcgg catcggcgcc gctggtccaa cccggaccac ttcgagcccg agcgttttgc
     4021 ccccgaacac gaggcggaga tcccgcgcta tcagtacatg ccgttcgggg cgggaccgcg
     4081 catctgcatc ggcatggcct tcgccatgat cgaaggcatc gccatcctgg caagcctggt
     4141 gcgagcggcg agctttgcgg tcacgccggg gcacgagccc gagccgatca gccgcgtcac
     4201 cttgcggccg gccggcggca tgccgctcgc cgtgcggctg cgctagcgcc gacgtaaaag
     4261 cccgcggttg acgcttgcga acctgccgat caaggctgct gggggcgcag cgtgcggcag
     4321 ggcagagctg cgaagggggg catggcagga tatggcagac taagagatcg cgccgagatc
     4381 tcaggaaaga ggggctcggc ggcgattacc cctaagaaca gcgcagggaa gggcgccgtc
     4441 ttagttcgcc agttttgcct tcagaaagcc gacgatcagc gccgtgaggc cgccgccgac
     4501 gccaccggtg acaaacgccg agacgatggc gccgacatcc agcccggagg cggcggcagt
     4561 tccagcaatc cccaacaccg cattgagcac ctggccgccg acgccgccgc cgatggcacc
     4621 ggcgatgcta ttgcccaacg gcccgagatt tatgtctttg gcaaggtttc cggcagcgtt
     4681 gccgccgagc gcgccggcga taagctgaat gatcagtgcc gtcaggttca tgaacgcctc
     4741 caccggccgg aacaaggaca ggccccggca actgcctcct ggagcgctcg ccccagcggc
     4801 ttgcggaaag cttttttggg cggcgtctct gtagtctcag caaaatatca cggcagcaga
     4861 cgatccgtcg ctcggtggca atgctgagga aatcgcgtgg tttttacggc attgctgagc
     4921 gttgcggggc atgcgaggcc acgatcgcgc gcccggccga cggcaagcgc gcgcaagatg
     4981 gctagcgcga cgggctctcg ccatttggc
//
LOCUS       DASTMT010000585         1522 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_4798771, whole genome shotgun sequence.
ACCESSION   DASTMT010000585 DASTMT010000000
VERSION     DASTMT010000585.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 1522)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1522)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1522
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4798771"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..554
                     /locus_tag="VFR00_13305"
     CDS             <1..554
                     /locus_tag="VFR00_13305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylenolpyruvoylglucosamine reductase"
                     /protein_id="HEU0160292.1"
                     /translation="LRMNAGAHGRETRDVLVEARAVDRQGRSHLLTSADMAFGYRHCG
                     VPEDLIFTEALYQGSPGSSADILRAMADVAEYREANQPIKERTGGSTFKNPPGSSAWQ
                     LIDQAGCRGLRIGGAKVSEMHCNFLINEGGATAEDIERLGETVRARVKAACGVTLQWE
                     IIRRGLARAGQPIGEALAEALSQ"
     gene            551..1495
                     /locus_tag="VFR00_13310"
     CDS             551..1495
                     /locus_tag="VFR00_13310"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013419721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEU0160293.1"
                     /translation="MTGTPHRFQHVAVLLGGLSAEREVSLRSGKACAEALEAAGYTVT
                     RIDAGRDLAAQLAQVRPQACFNALHGRWGEDGCVQGLLEIVGIPYTHSGVLASALAMH
                     KERAKSVMRAAGVPVPGGRIVARAEAARAHALERPYVLKPVAEGSSVGVYIVREDHAH
                     PPQELNDPRWAYGEDLLAEPFIAGRELTCAVLGDRALGVTEIIPAEALRFYNYEAKYA
                     DGGSRHLLPAPISSNVYELVQKLTLMAHRALGCRGVSRADFRYDDTPGGTGQLLCLEV
                     NTQPGMTGTSLVPELAAHAGLSLPELVSWMVEEASCNR"
BASE COUNT          254 a          471 c          546 g          251 t
ORIGIN      
        1 ccctgcgcat gaacgcgggg gcgcatggcc gcgaaactcg cgacgtgctg gtcgaggcgc
       61 gtgcggtcga tcggcaaggt cgcagccacc tgctgacgtc cgccgacatg gcattcggct
      121 atcgccattg cggtgtgccg gaggatctta tcttcaccga agcgctgtac cagggcagcc
      181 cgggcagctc ggccgacata ctaagggcga tggccgatgt cgccgagtat cgggaggcca
      241 atcagccgat caaggagcgc accggcgggt ccacgttcaa gaacccaccg ggctcgagtg
      301 cctggcagct gatcgaccag gcgggttgtc ggggcctgcg catcggtggc gccaaggtct
      361 cggagatgca ttgcaatttt ctgatcaatg aggggggcgc cacggccgag gacatcgagc
      421 ggctcggcga aaccgtgcgc gcacgtgtga aagcggcctg cggcgtcacc ttgcagtggg
      481 aaatcattcg ccgcgggctc gcccgcgcgg gccagccgat cggcgaagcc ttggccgagg
      541 cgctcagcca atgaccggca cgccgcatcg ctttcagcac gtggcggtgc tgctcggggg
      601 cctctcggcg gagcgggagg taagcttgcg ctcgggcaag gcctgcgcgg aggcgttgga
      661 agcggcaggc tacaccgtga cgcgcatcga cgccgggcgg gaccttgccg cacaactggc
      721 ccaggtgcgg ccgcaggcgt gcttcaacgc cctgcacggg cgctggggcg aagacggctg
      781 tgtgcaaggt ctgctggaaa tcgtcggcat cccctatacg cattccggcg tactagcctc
      841 ggccctggcc atgcacaagg agcgtgccaa atcggtcatg cgcgccgccg gcgttcccgt
      901 acccggtggt cgcatcgttg cgcgggccga ggcggcgcgc gcgcatgcgc tcgagcggcc
      961 ctacgtgctc aagcccgtgg ccgagggctc gagcgtcggg gtctatatcg tgcgcgagga
     1021 ccatgcccat ccgccgcagg agctcaacga tccacgctgg gcctatggcg aggacctgct
     1081 ggcggaaccg tttattgccg gccgcgagct cacctgcgcc gtgctgggag accgggcgct
     1141 tggcgtaacc gaaatcattc ccgccgaggc gctgcgcttc tacaactatg aagccaagta
     1201 tgccgacggc ggctcgcgcc acctgttgcc ggcgccgatt tcatcgaatg tttacgaatt
     1261 ggtgcagaag ttaacgctga tggcgcacag ggcgcttggc tgccgcggcg tttcgcgggc
     1321 ggactttcgc tacgacgaca cgccgggtgg cacgggtcag ctgctctgtt tggaggtcaa
     1381 tacgcagccg ggaatgacgg gcacatccct ggttccggaa ttagcggcac acgccggctt
     1441 gagtctgccg gagctcgtca gctggatggt ggaggaggcg agttgcaaca gataggacca
     1501 gaggcggaga tttggaggcg gg
//
LOCUS       DASTMT010000586         5548 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_5130291, whole genome shotgun sequence.
ACCESSION   DASTMT010000586 DASTMT010000000
VERSION     DASTMT010000586.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 5548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5548
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5130291"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1262)
                     /locus_tag="VFR00_13315"
     CDS             complement(<1..1262)
                     /locus_tag="VFR00_13315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018262800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEU0160294.1"
                     /translation="MRWNISAWSIRRPVPALVLFMVLLALGWTSFADLPVTRFPNIDV
                     PIVQVRVSQAGAAPAELEVQVTKRIEDAIAGVNGIKHQTSAVTEGASITTIEFRLEQN
                     QDRALNDVKDAISRIRSELPRTIDEPIITRLEIEGLPIVTYGARAPGKTPEELSWFVD
                     DVVIRALQGVKGVSQIERFGGAEREIRVALDPDRLLAFGITAGDVNRQLRAVNVDLAG
                     GRGEIGGREQAIRTLAGKQSLAELAATMISLPKGRKVRLDQLGTVSDGIAEQRAFASL
                     DGRAIVAFAISRGKGASDTAVAAAVAEKIVELRRAHPDVEFKQLDSMVSYTLGAYQST
                     MKTLLEGAILAIAVVFVFLRDWRATLISAIALPLSIVPAFWAIEAAGFSLNLVSLLAI
                     TIVTGILVDDAIVEIENIVRHIRMGKSP"
     gene            complement(1275..2462)
                     /locus_tag="VFR00_13320"
     CDS             complement(1275..2462)
                     /locus_tag="VFR00_13320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020090788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEU0160295.1"
                     /translation="MLRKLIIAVVVLLAAVGVALGLIMQPAPKRAPQASADAAPAAPV
                     TVVRVERAHFAETVLVTGSLAAREEVLVGPEVEGLRVTEVLADEGMRVKKGDVLARLV
                     ADTLEAQLAQNDAALARAEAASAQARSGIVQAEARLLEANNAFARAKPLRAAGHMPES
                     AFDQREQAARTAEAQLLAAREGLKVAEAEKGQVEAQRRELLWRRGRTEVIAPADGVVS
                     RRMARIGGFAAGSAEPMFRIVAQGEVELEAEIPETRLAAVRVGQPARVEVAGAGEVSG
                     SVRLVSPEVDKATRLGRVRIHLGDNPSLRVGAFARGSIETAVGEGLAVPLAAVLFGAD
                     GPVAQLIRDNRVETRKVQIGLSTGGMIEVRAGLSEGDLVVARSGTFLRDGDLVRPIPA
                     RTS"
     gene            complement(2501..3097)
                     /locus_tag="VFR00_13325"
     CDS             complement(2501..3097)
                     /locus_tag="VFR00_13325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEU0160296.1"
                     /translation="MPKLRPETQHARREHILDAAERCIANTGFHRTTMQDICRAAAIS
                     PGALYVYFDSKEALIAGISERDRQEFAERLEAMASAPDFMGALAALGQQYFLADAAKD
                     HRMAIDIGLEATRNQRVGEIFRRFDRYIVESFEALFEKLKAEGRIAPALDIPTTVKAF
                     LVVADGMFWRRAVDKDFDAERLLPAALKLIEALLNPSR"
     gene            3583..4788
                     /locus_tag="VFR00_13330"
     CDS             3583..4788
                     /locus_tag="VFR00_13330"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEU0160297.1"
                     /translation="MIPLLKGLRILDLTSVILGPYATQILGDLGAEIIKVEPPEGDSM
                     RPVAPVATPGISAIFANNNRNKRSVTLDLKSEAGRAALFKLLPNADALVHNMRQEAMD
                     KLGLTFKAVRAANPRIVYAAAVGFGRHGRYAGKPAYDDVIQAASGFAGLFEMRDGTPL
                     YAPTIAADKISGLHLAYALLAALMYRERSGEVPGYIEVPMFELMAAFSLSEHLGAATF
                     EDGGRVGYTRVLSRGRRPYKTKDGWIGVLPYTQRNWIKVLHEIGRAEVCEQPWFRDPT
                     ERSRRVDDLYDMLATALPARTSAEWLGRFKQLDIPSQPVRTPSDLLADPHLADVGFFA
                     PNFAGDTPVTRTLRQAVNVEHLPIEPDLPPPALGADTAEVLRAAGCSEDEIAQLVRKA
                     GGPSPVSTA"
     gene            complement(4804..5034)
                     /locus_tag="VFR00_13335"
     CDS             complement(4804..5034)
                     /locus_tag="VFR00_13335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0160298.1"
                     /translation="MHAHRLPCGTWLVIEAGTRDQGPLIVSRHASCQEAEAERDRRNR
                     NAGEPHHFQACLVLEPIAQRMGGRQSPAAESR"
     gene            complement(5216..5449)
                     /locus_tag="VFR00_13340"
     CDS             complement(5216..5449)
                     /locus_tag="VFR00_13340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0160299.1"
                     /translation="MITTKRCPHTGVVNFFVRADPYLAVGSVSQDANPRQFVWRCYLD
                     GQGAGVADDPAIAEANLRRAIARGRELAGLSVR"
BASE COUNT          921 a         1904 c         1797 g          926 t
ORIGIN      
        1 tacggcgatt tgcccatccg gatatggcgg acgatattct cgatctcgac gatggcatcg
       61 tcgaccaaga tgcccgtgac gatggtaatg gcgagcaggc tcaccaggtt aagggagaac
      121 ccggcggcct cgatggccca aaaggccgga acgatcgaaa gcggcagcgc gatggcagag
      181 atcagcgttg cccgccagtc gcgcaagaac acgaagacca cggcgatggc caggatggct
      241 ccctcgagca gggtcttcat ggtcgattga taggccccca gcgtgtagct gaccatgctg
      301 tcgagttgct tgaactcgac gtcaggatgc gcccgccgca gctcgacgat tttttcggcg
      361 accgcggcag ccactgccgt gtcgcttgcc cctttccccc gcgagatggc gaaggcgacg
      421 atggctcggc cgtcgaggct ggcaaaggcc cgctgttcgg cgatgccgtc gctgaccgtg
      481 cccaattgat cgagccgcac cttgcggccc ttcggcagcg agatcatggt ggccgcaagc
      541 tcggccagcg actgcttgcc ggcgagggtg cgaatggcct gttcgcgtcc gccgatctcg
      601 ccgcgaccgc cggcgagatc gacgttgaca gccctcagct gccggttgac gtcgccggcg
      661 gtgatgccaa aggccaacag gcggtccgga tcgagcgcga ccctgatctc gcgctcggcc
      721 ccgccgaagc gttcgatctg cgagacgcct ttcactccct gcagcgcccg tatcacgacg
      781 tcatcgacga accaggagag ctcctcgggg gtcttgccgg gcgcgcgcgc gccataggtc
      841 acgatcggca agccctcgat ctcgaggcgg gtgatgatgg gctcatcgat ggtgcgcggc
      901 agctcggagc ggatgcgcga gatcgcgtcc ttgacgtcgt tgagcgcgcg atcctggttc
      961 tgttccagcc ggaactcgat ggtggtgatc gaagcgcctt cggtcacggc cgacgtctgg
     1021 tgtttgatgc catttacgcc ggcaatggcg tcctcgattc gcttggtgac ctgcacctcg
     1081 agttcggccg gcgctgcacc cgcctggctg acgcgcactt gcacgatcgg cacgtcgatg
     1141 ttgggaaaac gtgtgacggg caggtcggca aagctggtcc agcccagcgc cagcagcacc
     1201 atgaagagca ccagcgccgg cacgggccgg cggatcgacc aagcggagat attccaacgc
     1261 atctcttggc ctttctagct ggtccgggcc ggtatgggcc gcaccagatc gccgtcgcgc
     1321 aaaaacgtgc cggagcgggc gacgaccagg tcgccctcgc tcaggccggc gcggacttcg
     1381 atcatgcccc ccgtcgacag gccgatctgg accttgcggg tctccacgcg gttgtcgcgg
     1441 atgagctgcg ccacgggccc gtcggccccg aacagcaccg cggccagcgg cacggcgaga
     1501 ccctcgccca ccgccgtttc gatgctgcca cgggcaaagg ccccgacacg caggctcggg
     1561 ttgtcgccca ggtggatgcg gaccctgccc agtcgcgtgg ccttgtcgac ctcgggcgag
     1621 acgaggcgca cgctcccgct cacctcgccc gcgccggcaa cctccacccg cgcgggctgc
     1681 cccactctga cagcggccaa ccgggtctcg ggaatttccg cctcgagttc cacctcgcct
     1741 tgcgcgacga tgcggaacat gggctcggcg ctgccggcgg cgaaaccgcc gatgcgcgcc
     1801 atacggcggc tgacaacgcc atcggccggc gcaatcacct cggtgcggcc acgccgccag
     1861 aggagttcgc gtcgctgagc ctcgacctgc cccttttcgg cctcggccac cttcaggcct
     1921 tcgcgcgcgg ccaggagttg cgcctccgcc gtgcgcgcgg cctgctcgcg ctggtcgaag
     1981 gcactttcgg gcatgtggcc ggccgcgcgc aatggcttgg cgcgcgcaaa ggcattgttc
     2041 gcctcgagca accgggcctc ggcctgaacg atcccgctcc gggcctgagc gctcgccgcc
     2101 tccgcgcgcg ccagcgcggc gtcgttttgt gccagctgag cttcgagcgt gtcggccacg
     2161 aggcgcgcga gcacgtcccc tttctttacg cgcatgcctt cgtcagccag aacttcggtg
     2221 acgcgcaagc cctcgacctc gggcccaacc agcacctctt cccgggctgc cagggacccc
     2281 gtaaccagca cggtctcggc gaagtgcgcc cgctcgaccc ggacaacggt gacgggcgcg
     2341 gcaggcgccg cgtccgcgga ggcttgcggc gctcgcttgg gcgcaggctg catgatcagg
     2401 cccagcgcaa cgcccactgc ggccaacagc acgacgacgg cgatgatcag ctttctcagc
     2461 atcacttccc tcacgcgggc cgcctctcct gcgaccctcc tcatcggctg gggttaagca
     2521 gggcctcgat cagtttgagc gcggcgggca acagccgctc cgcgtcaaag tccttgtcga
     2581 cggcccggcg ccaaaacatg ccgtccgcca ccaccaggaa ggccttgacg gtggtcggaa
     2641 tatcgagcgc tggggcgata cgcccctcgg ctttgagctt ttcgaaaagc gcctcgaaac
     2701 tttcgacgat gtagcgatcg aagcggcgga agatttcacc cacgcgttga ttgcgcgtcg
     2761 cctccaggcc gatatcgatc gccatgcggt gatccttggc cgcgtccgca aggaaatact
     2821 gttgcccgag cgcggcgagg gcccccatga agtcgggcgc gctggccatg gcctcgagcc
     2881 gctcggcgaa ctcttgccga tcccgctcgg aaataccggc aatcagcgct tccttgctgt
     2941 cgaaatagac gtagagcgcc ccggggctga tggccgccgc gcggcagata tcctgcatgg
     3001 tcgtgcgatg aaaaccggtg tttgcaatgc agcgctcggc ggcatcgaga atgtgctcgc
     3061 ggcgcgcgtg ctgcgtttcc ggtctgagtt ttggcactgt gaaacctcgt ccttcgaccc
     3121 gcacctgggc ccggccggac gctgggcgcc cgtgccgcat cgcacaaaac aaaacgaacg
     3181 gtcgttttat ttgcgaagga aggcggcgga gttagggcaa actccggcga ggccacgcgg
     3241 catggggcca caacgcaccg gtgcgggcgc cgaggctcgg cggacggctg gcgccacggg
     3301 gcaaagccag gaagaacgaa aagaaagaag gcacctcggc caagtgcgtt ggtgccctga
     3361 gttgaacaac gacccggctg tggagggaaa gcgaggccgg atctcgctag gaacgtgctc
     3421 ccggcccgcc cgttccctgc cgggcgccgg cctgcgagga atttcgcctg gccttggaaa
     3481 aagcgacgcc gcgcgcggag aggtcgccgg ggagcgctgt aggcgggccg cggctttgca
     3541 tgctaggtga cagggttcgc ccgtagctgc cgagccctag ccatgatccc tctactcaaa
     3601 ggtttgcgca tccttgacct gacgtcggtc atcctcggcc cctacgcaac ccagatcctg
     3661 ggcgatctcg gcgccgaaat catcaaggtc gagccgcccg agggtgacag catgcgaccg
     3721 gtcgccccgg tagccacgcc cggcatcagc gccatctttg ctaacaacaa ccgcaacaag
     3781 cgctccgtca ctctcgacct caagagcgag gcggggcgcg cggccctctt caagctttta
     3841 cccaacgccg atgcgctcgt gcacaacatg cgccaagaag ccatggacaa gctgggcctg
     3901 accttcaagg cggtgcgggc ggcgaaccca cgcatcgtct atgccgccgc ggtcggtttc
     3961 ggccggcacg gccgctacgc cggcaaaccc gcctatgatg acgtgatcca ggccgcctcg
     4021 ggctttgccg gcttgttcga gatgcgcgac ggcacgccgc tttatgcccc cacgatcgcc
     4081 gccgacaaga tttcggggct gcacctcgcc tatgcgctgc tggccgcgct catgtatcgt
     4141 gaacgcagcg gcgaggtacc cggctacatc gaggtgccga tgttcgagct gatggcagcc
     4201 ttcagcctga gcgagcacct cggcgccgcg acatttgagg acggcgggcg cgtcggctac
     4261 acgcgcgtcc tctcgcgggg gcggcgaccc tacaagacga aggacggctg gatcggcgtc
     4321 ttgccctaca cgcagcgcaa ttggatcaag gtcttgcacg agattgggcg tgccgaggtc
     4381 tgcgagcagc cctggttccg cgacccgacc gagcgcagcc gccgtgtcga tgacctttac
     4441 gatatgctgg cgacggctct gccggcgcga acctcagccg aatggctggg ccgtttcaag
     4501 cagctcgaca ttccctccca gcccgtgcgc acgccgagcg acctcttggc agatccacat
     4561 ttggccgatg tgggcttctt tgcccccaat tttgccggcg acacgccggt gacgcgcacg
     4621 ctgcgccagg cggtcaatgt cgagcatctg cccatcgaac ccgacttgcc gccgccggcg
     4681 ttgggcgcgg acacggcaga ggtcttgcgc gcggccggct gcagtgagga cgagattgcc
     4741 cagctcgtgc gcaaggcggg cgggccatcc cccgtcagca cggcctgatc gcccccgtgc
     4801 gcctcaacgg gattccgcag ccggggactg ccgcccgccc atgcgctggg cgatcggctc
     4861 gagcaccagg caagcctgga agtggtgcgg ctcgccggca ttccggttgc gccgatcgcg
     4921 ctcggcttcg gcttcttggc agctggcgtg ccgggaaacg atgagcgggc cttggtcccg
     4981 cgtccccgcc tcaattacca gccaggtgcc acaagggaga cggtgcgcgt gcatcggctt
     5041 tctcctccct gaattctaaa ggtcgcaggc aaaaccacga gggcgctctt gtcgcgtctg
     5101 ctgggctcgt gtcgcggacc tcttgttggg tgccggctcg tgctggccgg cgagcgttca
     5161 acgtctaggc actggtgcgc acggcgctaa agccagactg gccccgaggc cgccatcaac
     5221 gcacactgag gccggcaagt tcccggccgc gcgcaatcgc ccggcgcaag ttcgcctcgg
     5281 caattgcagg gtcatcggca acgcctgcgc cttggccgtc caggtagcag cgccagacaa
     5341 attgacgcgg attggcgtcc tggcttaccg agccaacagc cagataggga tccgcccgca
     5401 cgaaaaaatt gactacaccc gtgtggggac aacgcttcgt ggtgatcacg cccggcctcc
     5461 ttggtcgttc gtctctctcc ccttcccacg taccggcttg tatccccctc cgtcgacgct
     5521 acgctaccaa aacgaggcag tattaaca
//
LOCUS       DASTMT010000587         9317 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_6115872, whole genome shotgun sequence.
ACCESSION   DASTMT010000587 DASTMT010000000
VERSION     DASTMT010000587.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 9317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9317
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6115872"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            148..531
                     /locus_tag="VFR00_13345"
     CDS             148..531
                     /locus_tag="VFR00_13345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011924508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU0160300.1"
                     /translation="MRRTWTIIGVADVAYSLAWYQRLLGLPTSAPAHTDFAQILDADG
                     TVLLCLHQWGRHGHPPLTQPDPAPGNGLLLFFRVDDFDEALARARALVPRLEEEPHRN
                     PNTATMEFALRDPEGYYVMVSALAP"
     gene            712..1074
                     /locus_tag="VFR00_13350"
     CDS             712..1074
                     /locus_tag="VFR00_13350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU0160301.1"
                     /translation="MGNPFVHVELMTTDVGKAKTFYGKLFDWKLEDVAMSDMTYTMIR
                     VGEGTGGGLMKNPMPNATSLWVAYVDVADVKAATQKAKSLGAAVLKDVAEIEGAGSFS
                     IISDPTGALLGLWQAKKR"
     gene            complement(1130..2647)
                     /locus_tag="VFR00_13355"
     CDS             complement(1130..2647)
                     /locus_tag="VFR00_13355"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0160302.1"
                     /translation="MTVAISDRPYAWWKEPTKDQWLAWFAGFLGWMLDAFDFTIFFLI
                     MVPISQEFGVPLTLVAGTVTFTLWLRLVGAFGSGWLCDRIGRKTPLMISILWYSICNF
                     AAGFAPSFAFLATFRILLGIGMGAEWPAGAALAMEAWPARSRGIMSGLLQGAWGLGFL
                     LASLIYGLLFTSIGWRGMLWIGILPALLCVFIRYFVKESEVWQENNRKRLEQLSLAPA
                     GASGAAAPSLLVTVAGWLLGALGVAVVLFYILKSLAPPLLAGLVEPKLLGLLLDDKLM
                     GVVAAGLTPFVLPNLAALLGVSIPTQLAIFRPPFLWNTFTASMWMTGQFLVYYSMIAM
                     FASWLQKDLKLSPGDVALPNVLHNAIVFFAMGFWGFVGDHIGRRLAIIIPAIIGCFIA
                     PLYLNTTDLTWITIGFALQGAFAGAIYGLQPAYLTERFPTEVRGTASAFCYHFGTILG
                     GLVPPTVTFLAVDRGMGFPQPMLYGTIIGAVVAVIALALSPETKGMKFSSELQVK"
     gene            complement(2911..4026)
                     /locus_tag="VFR00_13360"
     CDS             complement(2911..4026)
                     /locus_tag="VFR00_13360"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011927340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HEU0160303.1"
                     /translation="MWTDRRYLDLAGIAEPIIQAPMAGANLAALAIAVSRAGGLGSLP
                     CGTLSVAAARRELDIIRQQTSAPINANFFCHALPPPDAARDRRWQARLAPYYAEFGLE
                     PDASAAPTRLPFDAAMCALICELRPRVVSFHYGLPEPVLLARLKAAGCVIQGSATTVE
                     EARWLEAHGADAVIAQGLEAGGHRGTFLSHDLGLQVGTFALVPQVVDAVRLPVIAAGG
                     IADARGIVAALALGASAVQLGTAYLLCPEAEISAPYRAVLRAADDQSTVITNVFSGRP
                     ARAILNRLIREVGPISADAPAFPLAAAGTQPLRKKAEALGSCAFSPVLAGQAAALARE
                     MGAGALTLALAHEAKSLLEGLAWPEATAPRPVPAAAS"
     gene            4140..5027
                     /locus_tag="VFR00_13365"
     CDS             4140..5027
                     /locus_tag="VFR00_13365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008962755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEU0160304.1"
                     /translation="METSDLKIFATVARLGGMNRAAIALNTVQSNVTARVRALEHKVG
                     CALFERQPRGVVLTTAGQRLLPYAEQILTLLEAAHRAARDDGEPQGTLLLGSLETTAA
                     LRLAPVLSRFVAAFPRVDLVLRTGTTAELVEAVLKRRLEGAFVCGPVEHGELEVAPVF
                     SEELVLLAAPAVASLAAAIDADTRMVVLKAGCSYRQRLESVLARRGLQTPRLLEFGTI
                     EAIFKCVGAGLGITLLPRSLVVRIRPKAEVSMHRLPRAEARVETLFVRRRDGVQSRAL
                     LAFLKCAAARGPQAQRRSA"
     gene            5212..6654
                     /locus_tag="VFR00_13370"
     CDS             5212..6654
                     /locus_tag="VFR00_13370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009763544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU0160305.1"
                     /translation="MSTTTVFRNAAWVIAWDDGRASHVYRRDLDVAIRGSDIIFMGKG
                     YAEAADTEVDCSERLLLPGLVNIHSHPTSEPLRKGITDESRSPGFWHSSLYEFLTVFN
                     NDPEGSIAAMRVALAELLLSGVTTVVDLSVPFDGWVDTRAESGIRGVIAPMFRDARWF
                     TKNGHQLDYAWDKKAGRDAFERARGLIDLAQQHPCQRLSGMLSPAQIDTCSPELIRDA
                     YDYAVERRLPLQIHASQSVTEFHEMFRRHGKTPIAWLNAIGALGRHTIIGHGIFLDHH
                     PWLHWPSRGDLGLLRDAGATVAHCPTVFMRRGIAMTTFGAYAREGIQLGIGTDTYPHN
                     FLEEMRSAFTISRAMAGTVDDVSTLEVFNAATLGGARALLREDIGRLKVGAKADLVLV
                     DIKHPAMMPVREPLRSLLYVAADRAVRDVYVDGVQVVADQRVLNIDYRAAAEALEEAQ
                     KRSMREVPKLDWAGRSADQLAPMVLPLQKG"
     gene            complement(6651..7337)
                     /locus_tag="VFR00_13375"
     CDS             complement(6651..7337)
                     /locus_tag="VFR00_13375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009462917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEU0160306.1"
                     /translation="MQLNADFSRRVAVHAAELPWTPSPIAGVDRRMLDRLGGEVARAT
                     SIVRYAPNSRFSAHTHDGGEEFLVLEGVFSDEHGDFPAGSYIRNPPTSRHTPGSTPGC
                     TIFVKLWQFALHDRTQVRIDTNAAAYTGSPQDTGVDVMALFRDEREEVRLERWAPRTE
                     IDRLLPGGGELLVLAGGFAEGGESFEPQSWLRLPAGARLRATAGASGCRLWAKLGHLG
                     EAPTGPPGRD"
     gene            complement(7422..8396)
                     /locus_tag="VFR00_13380"
     CDS             complement(7422..8396)
                     /locus_tag="VFR00_13380"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU0160307.1"
                     /translation="MVRTSTARAARLALVLAAGWLNWLGLAWQGTSAAAEPRPRGAFV
                     NVGTAAAPLRLWLEEMGRGAPILLIHGLGANTYSWRYLAPGLARSHRVLSVDLKGFGR
                     SDKPFDAAYGILDQARLLETLIAAKNLGGLTIVGHSLGGGVALALTVRLNLRRPGTVD
                     RLVLIDSIAYPQPVPLFIEVLRTPVLAEVGITAAPPELETFKGLLAAYADPDKITWDA
                     VRAYARPLYEPGGRHALLQTAQGIIPANLPALIAAYPTIQQPALLIWCADDRVVPVDV
                     GRRLARALPRAELSVFKGCGHVPQEEVPGLTLARLSSFLQHHPAPTQP"
     gene            8633..8839
                     /locus_tag="VFR00_13385"
     CDS             8633..8839
                     /locus_tag="VFR00_13385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007597424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PsiF family protein"
                     /protein_id="HEU0160308.1"
                     /translation="MRTSHVALALAGLLAIGGSALAQDKTKPARSAASLECSKQADEK
                     GLHGKARKSFRAKCLKDMKQKKAA"
     gene            complement(8957..>9317)
                     /locus_tag="VFR00_13390"
     CDS             complement(8957..>9317)
                     /locus_tag="VFR00_13390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008547600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU0160309.1"
                     /translation="GQMPRQVFDRAVAGSLCFGALKGGATVGFCRVITDRATFAYLAD
                     VFVDPGERGQGIGKGLMRAVTAHPELQNLRRWLLVTADAHGLYAQQGFVALATPERFM
                     ERCDPNVYLRLAAALEA"
BASE COUNT         1634 a         3005 c         3229 g         1449 t
ORIGIN      
        1 cgggcgccat gtcggcacgg aaatagcggc ctttcgcagc gcctgatgcg ccggctgcaa
       61 acctctgagg agcgccgacc atggcgcaag caggcgcgtg ttagacatcg aggccagtgc
      121 agcggaggtc ccgacccagg aggcgcaatg agacgcacgt ggaccatcat cggcgtggcc
      181 gatgtcgctt acagcttggc gtggtaccag aggctgctcg gcttgccgac atcggcgcct
      241 gcccacaccg actttgctca gatcctcgac gcggacggca ccgtgctgct ctgcctgcat
      301 caatggggca gacatgggca tccccccctc acccaacccg accctgcgcc gggcaatggt
      361 ctcttgttgt tcttccgggt tgatgatttt gacgaggcgc tggcgagggc gcgcgcgttg
      421 gtcccccggc tggaggagga gccgcaccgc aacccgaaca cggcaacgat ggaattcgcc
      481 ctgcgggacc cggagggcta ctacgtgatg gtcagtgccc tggccccctg aaggctgtga
      541 caaatccttg cgtgacgcga gcccgcggct tttgccagcc ctgggtgctc gccgagcgcg
      601 ggcgcggcgt tgcggcggac cgcaagcgcc ggcatttgcc ggggggaacg cgctggcggg
      661 gcccacggtt tagccctggt gatagcattc gcttttcttc cggaggtccc catgggcaat
      721 cccttcgtgc acgtggaatt gatgacgacc gacgtcggca aggccaagac cttctacggg
      781 aaactgtttg actggaaact cgaagacgtc gccatgagcg acatgaccta caccatgatc
      841 agggttggcg agggcacggg tggcgggctc atgaaaaacc cgatgccgaa tgcgacctcc
      901 ctatgggtgg cctacgtcga tgtcgccgac gtcaaggcgg caacgcaaaa ggccaagtcg
      961 ctcggcgcgg cggtcctgaa ggacgtggcg gagatcgaag gcgccggctc gttcagtatc
     1021 atcagcgatc ccaccggcgc attgctcggg ctgtggcagg cgaagaagcg ctaacggccg
     1081 gcgggcaggc gcgcgaggca ggcgagaggc ctgcccgcgc gtgccgtgcc tatttgacct
     1141 gcaactcgga gctgaacttc atccctttcg tctccgggct cagggcgagc gcgatgaccg
     1201 cgaccacggc gccgataatg gtgccgtaga gcattggctg ggggaacccc atgcccctgt
     1261 ccacggcaag aaaggtgacc gttgggggca cgaggccgcc caggatcgtg ccgaagtggt
     1321 aacagaatgc gctcgccgtg ccgcgcacct cggttgggaa gcgttccgtc agataggccg
     1381 gctgcaggcc atagatggcg cccgcaaacg cgccctgcag ggcaaacccg atcgtgatcc
     1441 aggtgagatc ggtggtgttg agatagagcg gcgcgataaa acagccgatg atggccggga
     1501 tgatgatggc caagcgccgg ccgatgtggt cgccaacgaa cccccaaaag cccatggcaa
     1561 agaagacgat ggcgttgtgc aggacgttgg gcagcgccac gtcgcccggc gagagcttta
     1621 aatccttttg cagccaggac gcgaacatgg cgatcatgga gtagtagacc aggaactggc
     1681 cggtcatcca catcgacgcc gtgaaggtat tccagaggaa tggcggtcgg aagatcgcga
     1741 gttgggtcgg aatcgagacg ccgaggaggg ctgccaggtt aggcaacaca aagggcgtca
     1801 gaccggccgc caccacgccc atcagcttgt cgtcgagcag caggccgagc agtttgggct
     1861 cgaccagccc ggccagcagc ggcggggcca gcgatttcaa gatatagaac agcacgacgg
     1921 cgacgccgag cgcgcccagc agccagcctg ccaccgtgac gagcagggag ggtgcggccg
     1981 cgcctgaggc cccggccggc gcgaggctca gctgctcgag ccgtttgcgg ttgttctcct
     2041 gccagacctc ggactctttc acgaaatagc gaataaagac gcacagcagg gccggtaaga
     2101 tgccgatcca gagcatgccg cgccagccga tcgaggtaaa aagcagcccg taaatcaggc
     2161 tggcgagcaa gaacccgagg ccccaggcgc cttgcaacag cccgctcatg atgccgcgcg
     2221 agcgtgccgg ccaggcttcc atggcaaggg cggcgccggc gggccattcc gcgcccatgc
     2281 cgatgcccaa gagaatgcgg aaggtggcaa ggaaggcaaa gctcggcgcg aacccggccg
     2341 cgaagttgca gattgagtac cagagaatag aaatcatcaa tggggttttg cgaccgatgc
     2401 gatcgcacag ccagccggag ccgaaggccc ccaccaggcg cagccaaagc gtgaaggtga
     2461 cggtgccggc gacgagggtc agcggcacgc cgaattcctg gctgatcggc accatgatca
     2521 ggaagaagat ggtgaagtcg aaggcgtcga gcatccagcc gagaaagccg gcaaaccagg
     2581 cgagccactg atccttcgtt ggctccttcc accaggcgta agggcgatcc gaaatcgcga
     2641 ccgtcatggc atttccctcc ccaaaaaata ggcgcgttgg gtcggttcag ccccgttttt
     2701 ctgggtaggc ttgttatgat ttcaaccgac cacctttgtg ggccacaacc tgctaacacc
     2761 acacgcgtta tgctaggccc ggaaatgctc ttcctacaaa tcaaatctgg cgccgtgcgg
     2821 tgtccattcc gcgcgcccca agcaacacgg ggggcaatgg tttaacgtgg cgcgccggct
     2881 acgcccgcgc cggcaatctc gcttaagaga ctaagaggcg gcggccggca ccggccgcgg
     2941 ggccgttgcc tcaggccatg cgagaccttc gaggaggctc ttggcctcgt gggccagcgc
     3001 cagcgtgagc gcgcccgcac ccatctcgcg ggcgagcgct gcggcctgcc cggccaacac
     3061 gggtgagaac gcgcaagatc ctaaggcttc ggcctttttc ctgagcggct gggtgccggc
     3121 ggcggccagc gggaaggctg gggcgtccgc gctaatggga ccgacttcgc ggatcaggcg
     3181 gttcaggatg gcgcgcgccg ggcggccgct gaagacattg gtgatgacgg tgctctgatc
     3241 gtcggcggcg cgcagcaccg cccgataggg ggcggagatt tcggcctcgg ggcacaagag
     3301 ataggcggtg ccgagctgca ccgcactggc gcccagggcg agggcggcga caatcccgcg
     3361 ggcgtcggcg atgccaccgg cggcgatgac gggaaggcgt acggcatcaa ccacctgcgg
     3421 caccagcgca aaggtgccca cttgcaggcc gaggtcatgg ctcagaaagg tgccgcgatg
     3481 gccgccggcc tcgaggccct gggcgatgac ggcatcggcg ccgtgcgcct cgagccagcg
     3541 cgcctcctcg accgtggtgg cggagccttg gatcacgcag ccggcggcct tgaggcgcgc
     3601 caggagcacg ggctcgggca agccgtagtg gaagctgacg acgcgcggcc gcagctcgca
     3661 gatcagcgcg cacatggcgg cgtcgaaggg caggcgggtg ggggccgcgg acgcgtcggg
     3721 ctcgaggccg aactcagcgt agtagggggc cagccgggcc tgccagcggc ggtcgcgcgc
     3781 cgcatccggc ggcggcaagg cgtggcagaa gaaattggcg ttgatgggcg cagacgtctg
     3841 ctgccggata atgtccaatt cgcgccgcgc cgccgcaacg ctcaaggtgc cgcagggcag
     3901 cgagccgagc ccgccggcgc gcgacacggc aatcgccagc gcggccaggt tggcaccggc
     3961 catgggtgcc tggatgatcg gctcggcgat gccggcaagg tcgaggtaac ggcgatcggt
     4021 ccacatcaca cgtctccggt gctgggcgag cttgcccagg ggcaccgagg ttaggccgtt
     4081 ttcacctatt ccatcgagcg atttgttttg ataattctta tcataaatcg agatatgtcg
     4141 tggaaacctc cgacctcaag atctttgcca ccgtcgcccg cctcggcggc atgaaccggg
     4201 cggcgatagc gctcaacacc gtgcagtcga acgtcacggc gcgggtgcgc gcgctcgagc
     4261 acaaggtcgg ctgtgccttg ttcgagcggc agccgcgcgg tgtcgtcctg accacggcgg
     4321 gccagcgcct cttgccctat gccgagcaga tcctgaccct gctcgaggcc gcgcacagag
     4381 ccgcccgcga tgatggcgag ccacagggta cgctgctgct gggatcgctg gagacgacgg
     4441 cggccttgcg gctggccccc gtgttgtccc gtttcgtggc ggcatttccg cgcgtcgacc
     4501 tcgtcttgcg cacggggacg acggcggagt tggtcgaggc ggtgctcaaa cggcgcctgg
     4561 aaggcgcttt cgtgtgcggc cccgtcgagc acggcgagct cgaggtcgcc ccggtgttca
     4621 gcgaggagct tgtgctgttg gccgcgcccg ccgttgcctc gctggcggcc gccatcgacg
     4681 ccgatacgcg catggtggtt ttgaaggcgg gctgctccta tcgccagcgg ctcgagagcg
     4741 tgcttgccag gcgaggcctg caaaccccgc gcctcctgga gtttggcacc atcgaggcca
     4801 ttttcaaatg cgttggtgcc ggtctcggca tcacgctgtt gccgcgcagc ctggtggtgc
     4861 ggatcaggcc gaaggccgaa gtcagcatgc accggctgcc ccgtgcagag gcgagggttg
     4921 agaccctgtt cgtacggcgt cgcgatggcg tgcagtcgcg cgccctgctc gccttcctca
     4981 aatgcgccgc ggcgcgcggc ccgcaagcgc agcgccgctc cgcctaagtg acgccgtggg
     5041 cggggagcgc tccgctctaa gccggggcgt ggcgggtgca cgccggcgcc ggcgcatccc
     5101 ctggaaaccc ttcccaaggc tcttgccgca ggtgacaatg ggcggggcac gcgcgccgcg
     5161 cgcgtctcgc cggcaaaccg ccggccggca agacagtgag attaagcgcc gatgtcgacc
     5221 accacggtct ttcgcaatgc agcctgggtc atcgcctggg atgacggccg tgccagccat
     5281 gtctatcggc gcgacctcga cgtggcgatc cgcggcagcg atatcatttt catgggcaag
     5341 ggctacgccg aggcggcaga caccgaggtc gattgctccg agcgcctgct gctgcccggt
     5401 ctcgtcaata tccattcgca tcccaccagc gagcccttgc gcaaggggat caccgacgag
     5461 agccgcagcc ccgggttctg gcacagttcg ctctacgagt tcttgacggt cttcaacaac
     5521 gatcccgagg gctcgatcgc ggcgatgcgg gtggcgctgg ccgagctcct cctcagcggt
     5581 gtcaccaccg tggtcgattt gtcggtgccg ttcgatggtt gggtcgatac gcgggccgag
     5641 tcggggatcc gcggcgtcat cgcgccgatg ttccgggatg ctcgctggtt caccaaaaac
     5701 gggcaccagc tcgactatgc ctgggacaag aaggctggcc gcgacgcctt cgaacgcgcg
     5761 cgcggcctga tcgatcttgc ccagcagcat ccctgtcagc gcctgagcgg catgctgagc
     5821 ccggcgcaga tcgatacctg ctcgcccgag ctgatccggg atgcctacga ttatgccgtc
     5881 gagcgccgcc tccccttgca aattcacgcc tcgcaaagcg tcaccgagtt tcatgagatg
     5941 ttccggcgcc acggcaagac cccgattgcc tggctcaacg cgatcggcgc gctgggacgg
     6001 cacaccatca tcggacacgg catctttctt gaccatcatc cctggctgca ctggccgagc
     6061 cgcggggatc tgggcttgct gcgcgacgcg ggagcgacgg tggcccattg cccgaccgtg
     6121 ttcatgcggc gcggcatcgc catgacgacc tttggcgcct acgcgcgcga aggcatccag
     6181 ctcggcatcg gcaccgatac ctaccctcac aatttcctag aggagatgcg cagcgccttt
     6241 acgatttcgc gcgcgatggc cggcacggtc gacgacgtga gcacgctcga ggtcttcaac
     6301 gcggcgaccc ttggcggcgc ccgcgcgctg ttgcgcgagg acatcggacg cctcaaggtc
     6361 ggcgccaaag ccgacctggt gctggtcgat atcaagcacc ccgcgatgat gcccgtgcgc
     6421 gaaccgctgc gaagcctgct ctatgtcgcc gccgaccggg ccgtgcgcga cgtctacgtc
     6481 gatggcgtgc aagtcgttgc cgaccaacgc gtcctcaaca tcgattatcg cgcggcggcc
     6541 gaggccctgg aggaagcgca gaagcgatcg atgcgcgagg tgccgaagct cgattgggcc
     6601 ggccgcagcg ccgatcagtt ggcgcccatg gtgctgcccc tgcaaaaagg ctaatcgcgc
     6661 cccggcggtc ccgtcggcgc ctcaccgaga tggccgagct tcgcccacag ccggcagccg
     6721 cttgcccccg ccgtggcgcg caatcttgcg ccggccggca agcgcaacca ggactgcggc
     6781 tcgaagctct cgccgccctc ggcaaatccg cccgccagca cgaggagttc accgccgcct
     6841 ggcaagaggc ggtcaatttc ggttctgggc gcccagcgct cgagccgcac ctcttcgcgc
     6901 tcgtcccgaa acaaggccat gacgtccacc ccggtgtctt gcgggctgcc ggtgtacgcc
     6961 gccgcgttgg tgtcgatccg cacctgcgtg cgatcgtgca aggcgaactg ccacagcttc
     7021 acgaagatcg tgcagcccgg cgtcgatccg ggggtatgtc gggaggtggg cgggttgcga
     7081 atgtaagagc ccgcgggaaa gtcgccgtgc tcgtccgaaa agacgccctc caggaccagg
     7141 aactcttcgc cgccgtcgtg ggtatgcgcc gagaaccggc tgttcggtgc gtagcgcaca
     7201 atgctggtgg cgcgcgccac ctcgccgcca aggcgatcga gcatgcgccg gtccacgccg
     7261 gcgatgggcg atggtgtcca gggaagctcg gcggcatgca cggcaacccg ccggctgaag
     7321 tcggcattga gctgcatcga caccccgtct gttgcggggg tccatgctag aagtggtggc
     7381 cagggcaggc aaggggcggg ccggagcgcg cgggctctgg ctcagggctg cgtgggcgcg
     7441 ggatggtgct gcaggaagga ggagagccgg gccagcgtta agcccggcac ctcctcctgg
     7501 ggaacgtggc cgcagccctt aaaaaccgac agctccgccc gcggcagggc gcgcgccaaa
     7561 cggcggccga cgtcgacggg aacgacgcgg tcatcagcac accagatcaa cagcgcgggt
     7621 tgctggatcg tcggataggc ggcgatcagg gccggcaggt tggcgggaat aatgccttgt
     7681 gcggtttgca ggagggcgtg gcgacctccc ggctcgtaga gggggcgggc gtaggcccgc
     7741 accgcgtccc aggtgatctt gtcggggtcg gcataggctg ccagcaagcc tttgaaggtc
     7801 tcaagctcgg gaggagcggc ggtgatgccg acctcggcca aaacaggggt acgcaacacc
     7861 tcgatgaaga gggggaccgg ctgcggatag gcgatgctat cgatgagcac cagccggtcc
     7921 accgtcccgg gtcgcctgag gttgagccga acggtcagcg ccagggcaac gccgccgccg
     7981 agggagtggc cgacgatcgt caaacccccg aggtttttgg cggcaatcag ggtttccagc
     8041 aggcgcgcct gatccaagat gccgtaggcc gcatcgaacg gtttgtcgga ccgcccgaag
     8101 cccttgagat cgacgctgag cacgcggtgc gaccgcgcca agccgggtgc caggtagcgc
     8161 caactgtagg tgttggcgcc caagccgtgg atgaggagga tcggcgcgcc gcggcccatc
     8221 tcctcgagcc acagccgcaa cggtgcggca gccgtcccca cattgacgaa ggcgccgcgc
     8281 gggcgtggct ccgccgcggc gctcgtcccc tgccaagcca ggccaagcca gttgagccac
     8341 ccggcggcaa gcaccagggc aaggcgagcc gcgcgtgctg ttgaggtgcg aaccatgccc
     8401 atccaattgc gtggcagacc tttaagggaa atcttggcgg cggaccgggg agcccgccgc
     8461 cagcgcgcga aaattgttcg cgctgtggcc gcttggtcac cggcgctgcc gatttccctg
     8521 ggctagccgc gaggcggcag cgtggggctg gaacattgtg ggcgtcgggg cgaacccaaa
     8581 gcgttgccgg acgttgttag ggggtggctt ttgccccgcg aaaggaaacg ttatgcgcac
     8641 ctcccatgtt gctcttgctc tggccgggct tctggctatc ggcggctccg cgctggcgca
     8701 ggacaaaacc aaacccgcgc gcagcgccgc atcgctcgag tgctccaagc aggcggacga
     8761 gaagggtctg catggcaagg cgcgcaagag tttccgggcc aagtgcctga aagacatgaa
     8821 gcaaaagaag gccgcctagc cgcgggcgag cccgatcgcg gccccgccgg ccatcccgta
     8881 acgcgcgcct tgcctcgacg cgcgtctgcc ctgggtgtgg ggcgtcgccg tggaggcgaa
     8941 agcctccttg gcggcttcac gcctcgagcg ccgccgccag ccgtagatag acgttggggt
     9001 cgcagcgttc catgaagcgt tccggtgtcg cgagcgccac aaacccttgc tgggcataga
     9061 ggccgtgggc atcggccgtg accagcagcc agcgccgtag gttttgcagc tcgggatgcg
     9121 ccgtgacggc ccgcatcagg cctttgccga tgccttggcc gcgctcaccg gggtcgacga
     9181 acacgtccgc gaggtaggca aaggtcgccc ggtccgtgat gacgcggcaa aagccaaccg
     9241 tggcgccgcc tttcagggcg ccgaaacaga gggagccggc gacggcacgg tcgaacacct
     9301 ggcgcggcat ctggccg
//
LOCUS       DASTMT010000588        11919 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_3810173, whole genome shotgun sequence.
ACCESSION   DASTMT010000588 DASTMT010000000
VERSION     DASTMT010000588.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 11919)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11919)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11919
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3810173"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            180..2537
                     /locus_tag="VFR00_13395"
     CDS             180..2537
                     /locus_tag="VFR00_13395"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU0160310.1"
                     /translation="MSPVAGSELPLDKVPAGVSVIPSALIERAATASIAETINTYVPG
                     ATINEALGNALATDLQYRGFSASPLNGTPQGLAIYQNGVRMNEVFGDTMNWDLIPQIA
                     IADIVIMSNNPAFGLNALGGSVNVIMKDGFSFQGATVDSRIGSFGHKEVAAEAGQRWG
                     NWATYVAGEWIDENGWRDLSPAAAKRAYADLGVKGSGAEFHLNYTFADTFLGVVGPTP
                     VELVEERRANVFTSPQSFDNRMQMVNLTGSVSVADTLKVSGNTYYRGFKQKRPDGNVS
                     EAIACDAAGPNAGLLCFEEADDVLFGRRADGTIVNVPIASLPNGVETVLGGNDRVSVD
                     SFSYGGAVQAVSKAHLFDRSNQFLLGTSLDLGRARVTSQSELGVLDPRSLAVSGLGII
                     IDQSLNPALDAGDVEVTPVDLVVRTSYYGLYFMNTLDVTDRLAVTVGGRYNLANIKLE
                     DQRGEDLNGDHTFQRFNPLIGATYKLLPGLTAYAGYSESNRAPTPAELACADPLRPCL
                     LENFLVSDPPLKQVVGRTIEAGFRGQVAAGYAGRDAMGAAKSNTLGWSLGYFRTLLND
                     DILTVASPIQGRGFFINGGDTLREGVEAALNYRSDRLFLYASYAFVNATFRNALEIAS
                     PDAPVGVPCSAFVADDPEDEVPNCAKVRPGDHIPSIPSHRLKFGFDYWVTPQWRIGAD
                     LIAVSNQFFRGDEGNDDRPLPGYAVVNLRTGYKVTDNVEVYGLVKNLFNKDYATFGTY
                     FDADALRNVAGDPVGVGRNATVLENPRTITPAAPFAVYGGVKVKF"
     gene            complement(2638..3621)
                     /locus_tag="VFR00_13400"
     CDS             complement(2638..3621)
                     /locus_tag="VFR00_13400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018316321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HEU0160311.1"
                     /translation="MQVADLVLPVFAVIVTGWLAGWLGYIPRSLADGLVHFAYNVAMP
                     ALLFITIAQEPARNLLEWRFLLAFGGGSILSFALVFLVIRIAWGCDLASSTIQGMAAA
                     MTNTGFVALPILHSIYGQPAVLPAAIATVFVAAVMFPASVILLESAHGGRHRASARPV
                     LLLKQITLNPMVLSTLLGLAWAIAGWPIPLPIATYLNIFAAALTPCALFAIGLGLTAA
                     GMRSKLKASMALTVVKLVIMPLIVYGLCSASGLNPLYTIAAVICAAVPTAKTVFILAG
                     AYKVEEELITATVSITTLLSVLTLLGWLYALSGLSAQVQRTAEALISTGQI"
     gene            3923..5110
                     /locus_tag="VFR00_13405"
     CDS             3923..5110
                     /locus_tag="VFR00_13405"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEU0160312.1"
                     /translation="MVAAVLKKPLRVAIVGGGIGGAAAACALNARNIAVRLFEQAPAL
                     AEVGAGVALQPNGIRMLRQLGLAEALLRCGARWRDPQYRRSDGSFIARMWPPELGNEI
                     EFYGMHRADLLHLFVERLRPDMVATDHKCVGFEQDEQEAIITFANGRREVADVVIAAD
                     GIHSRLQEFVVAPAAPLPSGSVAYRGLIPAASVAWPPGAMRNWLGTGKHFLVYPVRCD
                     ELINYVGFVRTDEQLRESWSAAGDPAALAQAFAGWDPMVEAITAHASMTFRWGLYDRE
                     PLPAWTRGRLTLLGDAAHPMLPHVGQGANQAIEDAVALATVLARADRSSAPQALRIYE
                     TLRRARTARVQLSARVNGARYDGASRDLGARDQQLAAQSQERAWIWNYDAGLEAAAAA
                     AAL"
     gene            complement(5165..5482)
                     /locus_tag="VFR00_13410"
     CDS             complement(5165..5482)
                     /locus_tag="VFR00_13410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0160313.1"
                     /translation="MLKAVLVASALMMAAFVGSAPQASAGVQPHLSKAPTDVLSTVGG
                     RHYHHRPFRHRHFGHFHRRGFVIGAPIYAYSYGGCSWLRHRALVTGSPYWWHRYRLCR
                     RGW"
     gene            complement(5802..6413)
                     /locus_tag="VFR00_13415"
     CDS             complement(5802..6413)
                     /locus_tag="VFR00_13415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006203039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HEU0160314.1"
                     /translation="MNLDFLITSLIIVASPGIGVLYTLAAGLSRGWRASVIAAFGCTL
                     GIVPHMLAAVLGLAALLHTSALAFQLLKYLGVAYLLYMAWSSLKEQGALKIEQEIGAR
                     SATQVIVSAILVNILNPKLSIFFLAFLPQFVSAEEPQPLARMLTLSAVFMLLTFVVFV
                     GYGVFAASVRTHVISRPQVLTWLRRSFAGAFAALGAKLALADR"
     gene            6709..7869
                     /locus_tag="VFR00_13420"
     CDS             6709..7869
                     /locus_tag="VFR00_13420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEU0160315.1"
                     /translation="MPNFPSSPPARPAIFWLAALVLLVTAAVLLRDVLLPFVAGLALA
                     YLLDPLVNQLERLGIYRGAATLLILVAFFAGAILLLLLALPVITAEITILIEKLPGYV
                     KGLQALAVDPSHPSISKAIGVGIEEAERSSGELASLGAGWLSEFLRTLWSDGRMLLSI
                     FSLLVVTPIITAYLVYDWNRIIATLDRLIPTAHADTVRALVREIDSTVAGFLRGQATI
                     CLILGIFYALALRALGLNHGLLIGAFSGLLGFVPYFGSLTGLLLSLGVAIAQFGLSWT
                     PILLVLGIFLVGQSLSDYVLAPYFVGNKVHLNPVWLMFALFSFGYLFGFVGLLIAVPL
                     AASIGVLVRFALRQQPASAVDCEVEAKLEAGVDLGKTLGERPAPRRPRTSAT"
     gene            8141..9334
                     /locus_tag="VFR00_13425"
     CDS             8141..9334
                     /locus_tag="VFR00_13425"
                     /inference="COORDINATES: protein motif:HMM:NF013044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HEU0160316.1"
                     /translation="MDRAAEINLLKTLLHYIDTKTTAMAAAPWHNEVTAYTCPERHKR
                     EQEILIRKRPLVMGLSCDWPTPGAYRTDDFAGVPILTVRGSDGKLRAFLNVCRHRGAK
                     IAQGVGSAGAFTCPYHGWTYGNDGRLRGLPEEATSFPGVRAERSGLTPLPLAEKHGMV
                     WVLPTAAADGFVDLAIDPWLDGLGADLASWKLETYHFHDRHVHYEDMNWKLLVDTFHE
                     GYHFGFLHKESLRDILVHNVGEFTPFGPNFRLVYGRTKLDRLKAMPESEWDLMWNTTI
                     VYSMFANTIFSPQGDHMEIFRIFPVEGRTDRAVMETSLYIPKPIETADEKRHWDANLA
                     LAIKVITTEDFPAGRTMQLGFASGAQSHVVYGRNEPALAHYHKSIRQALGLPVKEGPV
                     RAAAE"
     gene            complement(9436..11016)
                     /locus_tag="VFR00_13430"
     CDS             complement(9436..11016)
                     /locus_tag="VFR00_13430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018096219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     tetratricopeptide repeat protein"
                     /protein_id="HEU0160317.1"
                     /translation="MRFLFADCVLDTGRRELARGSQPVGVQPQVFDLLVYLVANRERV
                     VSKDDLLSAVWAGRIVSESTLISQISAARKAIGDNGEAQKLIRTIARKGFRFVGAVSE
                     EQSAGRAATAAGQLGRGEALTAPTLAPAVKPSLAVLPFENLSGDPDQDYFADGMVEDI
                     ITALSRLRWLFVIARNSSFIYKGRAVDVKQVGRELGVRYLLEGSVRKLTNRVRISGQL
                     IDATTGAHLWAERFEGSADDIFELQDQMTASVVGAIAPKLEQAESERATRKPTENLDA
                     YDYYLRGMLDLNRRSREATSSALHLFSKAIERDGDFASAYGMAAWCHVWRKYNGWMSD
                     RVQENSEGARLARRAVELGRDDAVALGRGGYALGFLAGEFDSGLVFLERALRLNPNLA
                     TTWVLSGLFRNFLGEPELAIEHLAHAMRLSPLDPTSYHMQAGTGFAHFLAGRFDEACK
                     WAERALREEPGCTPAAAVTAAAHALAGRLQEAQREMAHLRQVYPALRVTTFTGFTFRR
                     PEHLALWRDGLRKAGLPE"
     gene            11262..11612
                     /locus_tag="VFR00_13435"
     CDS             11262..11612
                     /locus_tag="VFR00_13435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0160318.1"
                     /translation="MCKRLAICLALMAFAAGPADAQQKEATLKRLNVPGAAFDILLAV
                     PKPQGATFEFSESPDALLVHLIGGELALGFDDVESMLKAAGTLRRPLAALRVESSEHG
                     LPIPVAVYLVPAGE"
BASE COUNT         2083 a         3801 c         3890 g         2145 t
ORIGIN      
        1 tggttgcacc aaagagcgcg ccaaaaaaga aggccgcgcg gcccccgcct cagccaggca
       61 cgacgccccc ggtggtggcg ccggccgcgg tggccccagc ccaatcccca tcccctggcc
      121 cggtcgccgc gccgtcagtg ccgccgccgc ccgccccggt caccgccgtc gaggtcagga
      181 tgtcgcccgt tgccggcagc gagttgccgc tcgacaaagt cccagctggc gtcagcgtca
      241 tcccctcggc gctgatcgaa cgggcggcca cggcctccat tgccgagacg atcaacacct
      301 acgttcccgg cgccaccatc aacgaggcgc tcggcaatgc gctggccacc gacctgcaat
      361 atcggggttt tagtgcttcc ccgctcaacg gcacgccgca gggccttgcc atctaccaga
      421 acggcgtgcg catgaacgag gtgttcggcg acaccatgaa ctgggatttg atcccgcaga
      481 tcgcgatcgc cgacatcgtc ataatgagca acaatccagc cttcggcctc aatgccttgg
      541 gcggctccgt caatgtcatt atgaaggatg gctttagctt ccagggtgcg acggtcgaca
      601 gccggatcgg ctcctttgga cacaaggagg tggcggccga ggccggccag cgctggggca
      661 actgggcaac ctatgttgcc ggcgagtgga tcgacgagaa cggctggcgc gacttgtcgc
      721 cggccgccgc caaacgcgct tatgccgacc tgggcgtcaa gggttcgggc gccgagttcc
      781 atctcaacta cacctttgcc gatacctttc tgggcgtcgt cgggccaacc ccggtcgagc
      841 tcgtcgagga gcgtcgcgcc aatgtcttca cctcgccgca gtccttcgac aatcgcatgc
      901 agatggtgaa cctgacgggc tccgtttccg tcgccgacac cctcaaagtt tccggcaaca
      961 cctactatcg cggctttaaa cagaagcgcc ccgacggcaa cgtgtcggaa gcgattgcct
     1021 gcgatgccgc ggggccgaac gcgggtttgt tatgtttcga ggaagccgac gacgtcctgt
     1081 tcggccgccg cgctgacggc acgattgtca acgtgccgat cgccagcttg cccaacgggg
     1141 ttgagaccgt tctcggcggc aacgatcggg tctccgtcga ttccttctcc tatggcggtg
     1201 ccgtgcaggc cgtcagcaag gcacatcttt tcgaccggtc caaccaattc ctgctcggga
     1261 ccagcctcga ccttggccga gcgcgggtga catcgcaaag cgagctgggc gtgctcgatc
     1321 cgcggagcct ggcggtgtcg gggcttggca tcatcatcga ccaatcgctc aaccccgccc
     1381 tcgacgccgg cgatgtcgag gtaacgcccg tcgatcttgt ggtgcgcacc tcctactatg
     1441 ggctctactt tatgaatacg ctggatgtaa cggaccggtt ggcggtgacg gtcggcggcc
     1501 gctacaacct cgccaacatt aaacttgagg atcagcgcgg tgaggatctc aacggcgatc
     1561 ataccttcca gcgtttcaat cccctgatag gtgcaaccta caaactgctg ccgggcttga
     1621 cggcctatgc gggctattcg gaatcgaaca gggctccgac gccagccgag ctggcctgcg
     1681 ccgatccgct gcgtccctgc ctgcttgaga acttcctggt gtccgaccct cccctcaagc
     1741 aggtggtggg taggaccatc gaggcgggtt tccgaggtca ggtcgcggcc ggctacgcgg
     1801 ggcgggatgc catgggcgcg gccaaaagca acacgctcgg ctggagcctt ggatatttcc
     1861 gcacgcttct caacgacgac attttaacgg tggcgagccc gatccagggc cgcggctttt
     1921 ttatcaacgg tggggatacc ctacgcgaag gggtggaggc ggcgctcaac taccggtccg
     1981 atcggctctt cctgtacgcg agctatgcct ttgtgaatgc caccttccgc aatgccctgg
     2041 aaatagcctc gcccgatgcc ccggtcggcg tgccctgcag cgctttcgtc gccgacgacc
     2101 ccgaggacga agtccccaac tgcgccaagg tgcgccccgg cgaccatatt ccaagcattc
     2161 ccagtcaccg cctcaagttc ggtttcgact attgggtcac gccgcaatgg cggatcggcg
     2221 ctgacctcat cgccgtgtcg aaccagttct tccgcggcga tgagggcaac gacgatcgac
     2281 cgctgccggg ctatgccgtg gtcaatttgc gcaccggata caaggtgacc gataacgtcg
     2341 aggtctacgg cttggtgaag aaccttttca acaaggacta cgcgacgttt ggcacctact
     2401 tcgatgccga cgccttgcgc aacgtcgcgg gtgatccggt gggcgttggg cgcaatgcca
     2461 cggtcctgga aaatccgcgc acgattacgc ccgcggcgcc cttcgccgtc tatggcgggg
     2521 tcaaagtgaa gttctgattg gagcggtgac agccgggaag cgatcacgcc cagcgacggt
     2581 ggaccggctc atgaagtccg ggaatgagcg caagcgcgcg gcgccatggc acttcggtca
     2641 gatttgcccg gtcgagatca gggcctcggc ggttcgctgc acctgggctg agagacccga
     2701 aagggcgtag agccagccca gcagcgtgag gaccgacagc agcgttgtga tcgaaacggt
     2761 ggcggtgatc agctcctcct cgaccttgta cgcaccggca agaatgaaca ccgtcttggc
     2821 agtcggcacg gcggcacaga taacggccgc aatcgtgtag agcggattga ggcccgatgc
     2881 cgagcacaga ccgtagacga tgagcggcat aatcacgagc ttaacgactg tgagggccat
     2941 ggaggccttg agcttcgagc gcatgcctgc cgccgtcagg cccaagccga tggcgaacag
     3001 cgcgcagggg gtgagcgcag cggcgaagat gttgagatag gtcgcgatcg gcagcggaat
     3061 gggccagcct gcaatcgccc acgccagacc cagcagggtc gataggacca tcggattgag
     3121 cgtgatttgc tttagcagca ggacgggccg cgccgacgcc cggtgtcggc cgccgtgtgc
     3181 gctctccagc aggataacgc ttgcgggaaa catcactgcc gccacgaaca cggtcgcgat
     3241 cgcggcgggc agaaccgcgg gctgaccgta gatagagtgc aggatcggca acgccacaaa
     3301 tccggtgttg gtcattgccg ccgccatgcc ttggatcgta ctgctggcga gatcgcatcc
     3361 ccaggcgatg cgaatgacca ggaataccag cgcaaatgag aggatggacc cgcctccaaa
     3421 cgcgagcagg aagcgccact ctagaagatt acgcgccggc tcctgcgcaa ttgtaataaa
     3481 cagcagcgcc ggcatcgcca cgttataggc aaaatgcacc agcccatcgg ccagagagcg
     3541 cgggatgtag ccgagccagc cggccagcca gccagtgacg atgactgcaa agacgggaag
     3601 taccaggtcg gcgacttgca tccggcgtcc tcgctgtttc cggttttttt taagtttgcg
     3661 agccggcagc ttgtgcccgc aacggctggg gccgtagagg ctcaaggggt aactgcacag
     3721 caaatccccg aggcctccta tcggggtctg cacctccttg gctgctgggc attgctgatt
     3781 tggcccgcgt gccgatgatg tcttggacga tgttccaccg gcaactctgc gtcggcgatg
     3841 gcgccatgcc aacggcgcgt gcgggtgctc tgggatggcg tttgagagca ggctaaactc
     3901 gaggctcccg agtgatctag cgatggtggc tgccgtgctc aaaaagccgt tgcgtgtagc
     3961 gattgttggg ggtggcatcg gcggcgctgc cgcggcctgc gcgcttaatg cgcgcaatat
     4021 cgccgtgcgc ctgttcgagc aggcgccggc tctggccgag gtcggtgccg gagtggccct
     4081 gcagcccaac ggcatccgca tgttgcggca acttgggctt gcagaggcgt tgctccgatg
     4141 cggtgcgcgc tggcgcgatc cacagtaccg ccgctcggac ggcagcttca tcgcccgcat
     4201 gtggccgccc gagctcggca acgagatcga attttacggc atgcaccgtg ccgatctgct
     4261 gcatttattc gtcgagcgcc tgcgcccgga catggtcgcg accgaccaca aatgcgtggg
     4321 cttcgagcag gatgagcagg aggcaatcat cacgttcgcc aacggccgac gggaggtcgc
     4381 cgacgttgtc attgccgccg atggcattca ttcgaggctg caggaattcg tcgtcgcccc
     4441 ggccgccccg ctgccatcgg gctcggtcgc ctatcgcggc cttattccgg ccgcgagcgt
     4501 cgcctggccg cccggtgcca tgcgcaactg gctggggacg ggcaagcatt ttctcgtcta
     4561 tccggtgcgc tgcgacgaac tcatcaacta cgtcgggttc gtgcgaacgg acgaacagct
     4621 gcgggagtcc tggtcggcgg cgggggatcc cgccgcgctg gcccaggctt ttgccggctg
     4681 ggacccgatg gttgaggcga ttacggcgca cgccagcatg acctttcgct ggggcctcta
     4741 cgaccgcgag ccgctgccgg cctggacacg tggccgcctg acgttgctcg gggatgccgc
     4801 gcatccgatg ctgccgcacg tcggccaggg tgccaaccaa gccatcgagg atgcggtcgc
     4861 attggcgacg gtactggcgc gcgcggaccg gagctcggcc ccgcaggccc tgcgcatcta
     4921 cgaaacgttg cgccgggcgc ggaccgcgcg cgtccagctc agcgcccgcg tcaatggcgc
     4981 gcgctatgac ggggccagcc gggaccttgg cgcacgcgac cagcagctcg ccgcccagtc
     5041 gcaagagcgc gcttggatct ggaactacga tgcgggcctg gaggcggccg cggcggccgc
     5101 cgcgctctga tcgccgcgga ccagtgagag acggaggccg cgcgggcgcg cggcctccaa
     5161 acccttacca gccgcgccgg cacaaccggt agcgatgcca ccaatagggg ctgcccgtca
     5221 cgagggcgcg atggcgcagc cagctgcagc cgccgtaact ataggcatag atcggagccc
     5281 cgatgacgaa cccccgccga tggaagtgac cgaagtggcg gtggcggaag gggcgatgat
     5341 ggtaatgacg cccgccaacc gtactcagca cgtcggtcgg ggccttgctg aggtggggtt
     5401 gaacgccggc cgatgcttgc ggcgccgatc cgacaaacgc ggccatcata agcgctgacg
     5461 ctacgaggac agctttgagc ataacaatgc ctctcactgt gtcccgagcg cgatccatcg
     5521 atggaacgga ggagacctat agcatccgtc gacggatcac tcgatttttc aagctgccgc
     5581 cgcgaagggt catctgcatg accgtcgcgg gcgcgagtgc ccgttatcgc cgcgcgcgac
     5641 gaaacgagtc aaaggcgtga cgggaggctc agctcgttaa gcttggagat gagcctaaca
     5701 ggcagagcct cgcggcgagg ccggcgccac gcgtgagcgt gcggctcgag gttttccgac
     5761 ctcggtgacc cgtgagcaca gcgtgcgcga gggcggccgc ttcagcggtc ggcgagggcg
     5821 agtttggcgc cgagtgctgc aaatgcaccc gcgaagctgc ggcgcaacca ggttagcacc
     5881 tgcgggcgcg aaattacgtg cgttcggacc gaggcggcaa aaacgccgta gccgacgaac
     5941 acgacaaacg tcagcagcat aaagaccgcg ctgagggtca gcatgcgggc caaaggctgg
     6001 ggctcttcgg cgctgacgaa ctgcggtagg aaggccagaa agaaaatcga cagttttgga
     6061 ttgaggatat tgaccaggat ggcggaaacg atcacctgcg ttgccgagcg ggcgccgatc
     6121 tcctgctcga tcttgagcgc gccctgctcc ttgagcgaac tccaggccat gtacaagaga
     6181 taggcgaccc cgagatattt gaggagttgg aacgccagcg cgctcgtgtg caggagggcg
     6241 gcgaggccca gcacggccgc cagcatgtga ggaacgatgc ccagggtgca tccgaaggct
     6301 gcgatgacgc ttgcgcgcca accgcgggag agcccggccg ccagcgtata gagaacgccg
     6361 atgccgggcg aggcgacaat gatcaatgag gtgatcagaa aatcgaggtt cacgggccgc
     6421 ggctcctccg ttggggatcg gagcatgtct cgcccggccc cggccggcaa gagcgttcaa
     6481 ccgcgggctc gggcgagctg gccatccccg gcgcgggggg cgcgggcgcc cgcgccgacc
     6541 ttggcctcgc ccgcgctggg ccgccgttgc cgcgccccga cactggtgaa cggctggcgg
     6601 accccccaat cacagccccg gcgtgtcgtc agggcattcc ccgattgccg cggcggcaca
     6661 cctgagcgag ccatacgcgc ggggggcgac agaataggca acgccgccat gcccaacttc
     6721 ccatccagcc cgcccgcccg cccggcgatc ttttggctgg ccgcccttgt gcttctggtc
     6781 acggcggccg tgttgctgcg cgacgtgttg ctgccgtttg tggcgggtct ggcgctcgcc
     6841 tatttgcttg acccgctcgt caatcagctg gagcgcttgg ggatctaccg gggcgcggca
     6901 acgctgctta ttctggtcgc cttctttgcc ggcgcgatcc ttttgcttct gctggcgctg
     6961 cccgtcatca ccgccgaaat caccatcctg atcgaaaagc ttccgggcta cgtcaaaggg
     7021 ctgcaggctt tggccgtcga cccgagccat ccctcgatca gcaaagccat tggtgtcggg
     7081 atcgaagaag ccgaacgatc aagcggcgaa ttggcgagcc tcggcgccgg ctggctgtcg
     7141 gagtttctgc gcacgctttg gtcggatggc cggatgctgc tctccatctt ttcgctgctg
     7201 gtcgtgacac cgatcatcac cgcctatctg gtctacgatt ggaaccgcat tatcgcgacc
     7261 ctcgacaggc tcattcccac ggcccacgct gacacggtca gggcgctggt gcgcgagatc
     7321 gatagcacgg tggcgggctt cctgcgcggg caggccacca tctgtctgat cctcggcatc
     7381 ttctacgccc tggcgctgcg ggccttgggt ttgaaccatg gcctgttgat cggtgccttc
     7441 tccggcctgc ttggatttgt cccctatttc ggctcgctca ccgggctttt gttgtcgctg
     7501 ggcgttgcca ttgcgcagtt tgggctgagc tggacgccaa tcctgttggt gcttggcatt
     7561 ttcctggtgg ggcaatcgct gtcggattat gtgttggccc cctatttcgt gggcaacaag
     7621 gtccacttga accccgtctg gttgatgttt gcgctctttt cctttggcta tctgtttggc
     7681 tttgtcgggc tgttgattgc ggtccctttg gcggcctcga tcggggtgct cgtgcgcttt
     7741 gccctgcgtc agcagccggc aagcgccgtc gactgcgagg tcgaggcaaa gctcgaggcg
     7801 ggcgtcgact tgggcaagac gctgggcgag cggccggcgc cgcgacgccc gcgaacctct
     7861 gccacgtagt gtgatcggcc agcttgatcc gacagcgtga tcggcggcgt ggcttcggct
     7921 ggtcgacgcc cttttggccc gtcgcgcttg tcggcaatcg gcgggccgtc gagcttggcg
     7981 atgagcgcgc gggcctggcg ccagcaaaat cgcgggttcg tgccggccgc tggcaacagt
     8041 cgcacgggag ccgttggcct cgcgggggcg caaaggggct agcgcgccgg gcttggcgga
     8101 gactagaatt cggccctctt caacccggcg agcactgaat atggaccgcg cggcagagat
     8161 caatctttta aagacgctgc tgcattacat cgacaccaag acgacggcga tggcggcggc
     8221 gccatggcac aacgaggtga cggcctacac ctgtcccgag cggcacaagc gggaacagga
     8281 aattttgatc aggaagcggc cattggtcat ggggctttct tgcgactggc ccacgcccgg
     8341 cgcctatcgc accgacgatt tcgccggcgt accgatcctg accgtgcgcg gtagcgatgg
     8401 gaagctgcga gccttcctca atgtctgccg ccaccgcggc gccaagattg cccagggcgt
     8461 gggcagcgcg ggcgccttca cctgccccta tcacggctgg acctacggca acgacgggcg
     8521 gctgcgcggg ttgcccgagg aggcgacgtc ctttcccggc gtgcgggcgg aacgatcggg
     8581 tctgacgccg ctgccgcttg ccgagaagca cggcatggtc tgggtgttgc cgacggccgc
     8641 cgccgacggc ttcgtcgacc ttgcgattga tccgtggctc gacggcttgg gtgccgacct
     8701 ggcttcctgg aagctcgaga cctaccactt ccacgatcgc cacgtgcact acgaggacat
     8761 gaactggaaa ctgctcgtcg ataccttcca tgagggttat catttcggct ttctgcacaa
     8821 agaaagcctg cgcgacattc tggtgcacaa tgtcggcgag ttcaccccgt tcgggccgaa
     8881 tttccgcctg gtctacggcc gcaccaagct tgaccggctg aaagccatgc cggaaagcga
     8941 gtgggacctg atgtggaaca ccaccatcgt ctattcgatg tttgccaaca ccatcttttc
     9001 cccacaaggc gaccacatgg aaatattccg aattttcccg gtggagggtc gcaccgatcg
     9061 ggcggtgatg gagacctcgc tttacattcc caagccgatc gaaacggcgg acgagaagcg
     9121 ccactgggat gccaaccttg cgctggccat caaggtgatc acgaccgaag acttcccggc
     9181 cggccgtacc atgcaacttg gcttcgcctc gggtgcgcaa agccacgtgg tctacgggcg
     9241 caacgagccc gcgctcgcgc actaccacaa atcgatccgc caggcgctcg gcttgcccgt
     9301 gaaggagggc ccggttcgcg ccgcggcgga atagcatcgc gccagcaacg gcgttggggt
     9361 ccggcgcgcc aagcgtcaag gcgagcctgc agccgcagcg agcggcgatc atccctcgct
     9421 ggcgcaggcc aagccctatt cgggcagccc agccttgcgc agaccgtcgc gccagagcgc
     9481 caggtgttct gggcggcgaa aggtgaagcc ggtgaaggtc gtcacccgca acgccggata
     9541 aacttggcgc aggtgggcca tttctcgttg cgcttcttgc agccgacccg caagtgcgtg
     9601 cgccgcggcc gtgacggcgg cagcgggcgt acagcccggc tcttcgcgca atgctcgttc
     9661 tgcccattta caggcttcgt cgaagcggcc ggccaggaaa tgcgcaaagc ccgtccccgc
     9721 ctgcatatgg tacgaggtgg gatcgagggg gctgaggcgc atggcgtggg ccaagtgttc
     9781 gatggcaagc tcgggctcgc ccagaaagtt tctaaacagg ccgctgagga cccaggttgt
     9841 cgccaggttg ggattgagcc gcagcgcccg ctcgaggaaa acgaggccac tatcgaactc
     9901 gccggcgaga aagcccagcg catacccgcc ccgtcccagc gcgaccgcat catccctgcc
     9961 cagttcaacg gcccgccgag ccagccttgc gccctcgcta ttttcctgaa cgcggtccga
    10021 catccagcca ttgtatttgc gccagacgtg gcaccatgcg gccatgccgt aggccgaggc
    10081 aaaatcgccg tcgcgttcaa ttgcttttga gaatagatgc aaggcactgc tggtggcctc
    10141 gcggctgcgc cgattgaggt cgagcatgcc gcgcaaatag tagtcgtagg cgtcgaggtt
    10201 ctctgtcggc ttgcgcgtgg cgcgttcgct ttctgcctgc tctagcttcg gggcgatggc
    10261 gccgacgacg cttgccgtca tctggtcctg aagttcgaag atatcatcgg cgctaccttc
    10321 gaaacgttcg gcccaaagat gcgcaccggt ggtggcgtcg atgagctgcc cgctgatgcg
    10381 cacgcggttc gtgagcttgc gcacgctgcc ttcgagcagg tagcgcacgc ccagttcacg
    10441 cccgacctgt ttcacgtcga cggcgcggcc tttgtagata aagctcgagt tgcgcgcgat
    10501 gacgaacaac cagcgcaggc gggagagggc ggtaatgata tcttcgacca tgccgtcggc
    10561 gaaatagtcc tggtcggggt cgccgctgag gttttcgaag ggcaacacgg caagggacgg
    10621 cttgacggca ggcgccagcg tcggtgctgt tagcgcctcg ccgcggccaa gctgcccggc
    10681 cgctgtcgcg gcgcggccag cggattgctc ttcgctgacc gcgcccacga aacgaaaccc
    10741 cttgcgggcg atggtgcgga tcagcttttg cgcttcgccg ttgtcgccaa tcgccttgcg
    10801 cgcggcgctg atctggctga tcagcgtgga ctccgagacg atgcggcccg cccacaccgc
    10861 cgacagcaga tcgtccttgc tgacgacacg ctcacggttc gcaacgagat agaccaggag
    10921 atcgaacact tgcggttgca cgccgacggg ctgcgacccg cgcgcgagct cgcgccggcc
    10981 ggtgtcgagc acacagtccg cgaataaaaa ccgcaccttg cccaccccat gccgagggcg
    11041 cctgcaccgt gcacaggctg cgcctgcgcc cggcattgat agcacgccgg ccagcaaacc
    11101 ttcagcaacc ccatcttcga ggtttgggcg cgcgtctaca aatcggcaag gcaaattcaa
    11161 gcataaagca agcccacatc aaggtcttcg ggcggggtgt gggctagcct gttgtccggg
    11221 gcgatggcga tggaaacggt cgaccggcaa tggaggtccc cttgtgcaag cggctcgcga
    11281 tctgtctcgc attgatggcc tttgccgcgg gcccggccga tgcccagcag aaagaggcca
    11341 cactcaaaag gttgaatgtg ccgggagccg ccttcgacat cctcctcgcc gtgccaaaac
    11401 cccaaggggc aaccttcgaa ttctccgagt ccccggacgc gctgctggtg catctgatcg
    11461 gcggtgagct cgcgctcggc ttcgacgatg tcgaaagcat gctcaaagcc gctggcacgc
    11521 tgcgccgacc gttggctgcc ctgcgggtgg agagctctga gcacgggctg ccgatacccg
    11581 tggcggtcta ccttgttccg gcgggcgagt aggtcgcgtc tccccggccg agccgctccg
    11641 gtgcgttcgg tcgttttttt tccacaacgg gcctgagctt gcatcgcccg gtgctgccgg
    11701 cgaacgggtt ggcgctggcc aggcaaagat cgatggcctg gcggcctcga ccttcgggcg
    11761 ggccgcttgt cgcttcgaag tcgctgtgga gtatgcgtca gcggtgattg cgaggcgctg
    11821 agggcatgtt agagaggggg actcggctag agggccctga tgcgctatct cggcatcgtc
    11881 attttcgtcc tcctgtgcag tcttgagctc aattcggca
//
LOCUS       DASTMT010000589         1646 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_4803105, whole genome shotgun sequence.
ACCESSION   DASTMT010000589 DASTMT010000000
VERSION     DASTMT010000589.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 1646)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1646)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1646
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4803105"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..573)
                     /locus_tag="VFR00_13440"
     CDS             complement(<1..573)
                     /locus_tag="VFR00_13440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011925219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HEU0160319.1"
                     /translation="MRDPYEVLGVPKGASAAEIKSAYRKLAKKLHPDANKNDAKAASR
                     FAELNAAHEIVGDAEKRKAFDRGEIDAEGKPRFQGFGGFGAGRGSSSGFGGFGGGEGS
                     FESFTFGPEGFQRASGGRGGGFEDILKGMFGGRAGGRGGRASGFQFDAEDFVEPGGRD
                     ITASLTITLPEAAAGVSKRVLLPTGKEVEVK"
     gene            754..>1646
                     /locus_tag="VFR00_13445"
     CDS             754..>1646
                     /locus_tag="VFR00_13445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0160320.1"
                     /translation="MRRISASLLVALASATACASAAPARAQAADAFYKGKTLTMVVST
                     AGGEGFGLNGRLVATYMGRFLPGHPTVVTKYMPGAGHVLAANYLATDAPRDGTVIATI
                     APNIILHQLLDGRGVRYDIAKFNWLGASEYGNQAVYAWAATGLKTLHDAMTRQVLAGG
                     TGAGSFTVLYPTLMNNLLGTKFKLVTGYKTTRDIDLAMQRGEVEVRAGQSFTSLKAYN
                     GDWLREKKINVIAQVGREREPDFADVALLTDYAKTDATRRILELFEVQLTVGRPFLAP
                     PDVPADRLAILRKAFDQTMHDP"
BASE COUNT          280 a          602 c          491 g          273 t
ORIGIN      
        1 cttgacctcg acctctttgc cggtcggcag cagcacgcgc ttgctcaccc cggcggcagc
       61 ctccggcaac gtgatggtca gcgaggcggt aatgtcacgc ccgccgggct cgacaaagtc
      121 ctccgcatcg aactggaagc cgctggcgcg cccgccgcgg ccgcccgcac ggccgccgaa
      181 catgcccttg aggatgtcct cgaagccgcc gccccggccg ccgctggcgc gctgaaagcc
      241 ctcggggccg aaggtgaagc tttcgaagct gccctcccct cccccgaagc cgccaaatcc
      301 gctggagctg ccacggccgg cgccaaagcc cccgaaaccc tggaagcgcg gcttgccctc
      361 ggcgtcgatc tcgccgcggt cgaaggcttt gcgcttctcg gcatcgccga cgatctcgtg
      421 ggcggcgttg agctcggcaa accggctcgc agccttggca tcgttcttgt tggcatccgg
      481 atgcagcttc ttggcgagct tgcgatacgc gctcttgatc tccgccgcgc tcgcaccctt
      541 gggcaccccc aacacctcat agggatcgcg catccttcga acctctggat tgacctttcc
      601 tcatgtcgtg ccgttcaccc gaccaatcac tgaatgtggg ggccaacgcc cccgactgca
      661 acgcgaatcc ccgcttgcga cgatccagcc cgggcgactc atcatgcctg atggtgtagc
      721 atccttgcgc cccgcaatct ccggagcaga agaatgcgca ggatttcggc atccctgctg
      781 gtcgccctcg cgagcgccac cgcctgcgcg tcggccgccc ctgcccgcgc ccaggccgcg
      841 gacgcattct acaagggcaa gaccctgacc atggtcgtca gcaccgcggg cggcgaaggg
      901 ttcggcctca acggacggct ggtcgcgacc tatatgggcc ggttcctgcc tgggcatccg
      961 accgtcgtca ccaaatacat gcccggcgcc ggccacgtgc tcgccgccaa ctacctcgcg
     1021 accgatgcgc cgcgcgacgg cacggtcatc gccaccatcg ctcccaacat catcctgcac
     1081 cagcttctcg acggccgcgg cgtgcgctac gacatcgcca aattcaactg gctcggcgcc
     1141 agcgaatacg gcaaccaagc ggtctatgcc tgggcggcaa cgggcctgaa gacgctccac
     1201 gatgccatga cacggcaggt gctcgcgggc ggcaccggcg ccggctcgtt tactgtgctc
     1261 taccccacgc tcatgaacaa cctgttgggg acgaagttca agctcgtgac cggttacaag
     1321 accacccgcg acatcgacct tgccatgcag cggggcgagg tcgaggtgcg cgccggccag
     1381 tccttcacca gcctcaaggc ctacaacggc gactggctgc gggagaagaa gatcaatgtg
     1441 atcgcccaag tggggcgcga gcgggagccg gatttcgccg acgttgcttt gctgacggac
     1501 tatgccaaga cagatgcgac gcgcagaatc ctggaactgt tcgaagtgca gctcaccgtc
     1561 ggccgtccgt tcctggcccc gccggatgtg ccggccgatc gcctggcgat cctgcgcaag
     1621 gctttcgacc agaccatgca tgatcc
//
LOCUS       DASTMT010000590         6141 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_5133629, whole genome shotgun sequence.
ACCESSION   DASTMT010000590 DASTMT010000000
VERSION     DASTMT010000590.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 6141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6141
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5133629"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..749
                     /locus_tag="VFR00_13450"
     CDS             <1..749
                     /locus_tag="VFR00_13450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011581400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEU0160321.1"
                     /translation="LAEPSLIGISGGAALGAVLAIHLGITQALALALPLGGLLGAFIA
                     VLAVVALAGLNGGPITLILAGLAVSATTTALISLALNLSRNPFASIEMVYWMMGSLAD
                     RSLTQAWLSGPLMLIGMGLSLLLGRALDALSLGEDAAVSLGIDARRARLTLVAGTALS
                     VGAATAVTGIIGFVGLLVPHALRPLTGHRPGLLLPASLFGGAILVLLADVALRLVQPW
                     VDLRIGVLTALFGAPFFIWLVLKTRRELAP"
     gene            746..1519
                     /locus_tag="VFR00_13455"
     CDS             746..1519
                     /locus_tag="VFR00_13455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011581401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0160322.1"
                     /translation="MKLEARGLRVHLRQREVLRGVDLAAAAGELTAVIGANGAGKTTL
                     LRTLSGLLPFSAGEARLDQRPLPAWPRPELARCLAYLPQERTVHWALSARAVVALGRL
                     PHQTLGQGETVADRAAIAAAIAAMDIDALIARPVLELSGGERARVLLARALAQEPQVL
                     VADEPVAGLDPAHQLALFHHLRVFAASGRSVLVALHDLALAARFCHKIVLLKAGAVLT
                     SGPPSQVLTREHLASAYDIRAEYRTIDGIATVLSRDGLP"
     gene            1580..2659
                     /locus_tag="VFR00_13460"
     CDS             1580..2659
                     /locus_tag="VFR00_13460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEU0160323.1"
                     /translation="MLRLWRRQRRVNLALEGGGAHGAFTWGVLDRLLEEDQLEIGWLS
                     GTSAGAVNAVALAAGLAEGDRTRARAKLREVWEAVAKAGVPDLMRLNPWLASLSRSQT
                     LAQVASLFSPYDFNPLGFDPLRKVLEAHIDFALLRTQPGPELLIAATDVQSGRPRLFR
                     RRELKVECVLASACLPTLHHAVSVDGRAYWDGGFTANPDLVTLAREAPVADTLIIKLN
                     PMLVESLPTSAREISARINHITFNQPMLRDVELIESVRRNHKGWPRLRRDDDRRLARH
                     RFHLIEAGRVTGALSPESKVKPDLDLLLHLCGAGRSETEKWLARHTRAIGRRSSVDLA
                     QHFLAPRPSTAAASSPPRPKPEAAA"
     gene            2759..3511
                     /locus_tag="VFR00_13465"
     CDS             2759..3511
                     /locus_tag="VFR00_13465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018319829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU0160324.1"
                     /translation="MTDRLKGKLAFCTASAAGIGRATAIAFAREGARVIASDINKAGM
                     AGLTEAGVAECVALDARDAAAIEAQAKRLGAVDVLFNAAGFVHHGSVLDATDQEWDFA
                     FDLNVKSMHRTIKAFLPDMLQAGRGSIVNIASTVGAAKAAPNRYIYGASKAAVVGLTK
                     AVAIDFIGKGIRCNCICPGTIATPSLDERIRALGRQLGSEEKAREMFIARQPMGRLGT
                     AEEIAYVAVYLASDESAYMTGSAIIPDGGFTL"
     gene            3541..4317
                     /gene="otnI"
                     /locus_tag="VFR00_13470"
     CDS             3541..4317
                     /gene="otnI"
                     /locus_tag="VFR00_13470"
                     /EC_number="5.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HEU0160325.1"
                     /translation="MPRFAANLNFLFTDLPFLDRFDAAAKAGFKAVESTDLYSATAKE
                     VAARLRANALELALFNVNPGDPNKGERGVAALPGREADFAARFETALSYAEATGGRLI
                     HVMPGLVHQGARRDVYLANLKKAARQAAAAGAQIVIEPINRRDIPGYFLNKTHEARAI
                     IHEVAEPNLGLQFDLYHRQIEEGDVAMAIDEFGPLARHYQIASPPDRGEPDAGEMNYP
                     WLFQKIDASGFKGYIGCEYRPRGDTAAGLKWAAACGVSLG"
     gene            4334..5308
                     /gene="denD"
                     /locus_tag="VFR00_13475"
     CDS             4334..5308
                     /gene="denD"
                     /locus_tag="VFR00_13475"
                     /EC_number="1.1.1.410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014495981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-erythronate dehydrogenase"
                     /protein_id="HEU0160326.1"
                     /translation="MNILIIGGAGMIGAKLAGRLARDGTLNGKPITSLSLYDVVPPAA
                     PAATKMPVKVMTGDLPTPGEAGKLMADRPDLIFHLAAIVSGEAELEFDKGYRINLDGT
                     RLLLEAIRAAGHTPRLVFTSSIAVFGAPFPEAIADEFFNTPLTSYGTQKTICELLISD
                     YTRKGFLDGLSIRLPTICVRPGKPNKAASGFFSNIMREPLAGEAAVLPVSEEVRHWHA
                     SPRAAVGFLLHAASLDTSTVGPRRALTMPGVSVTVGEQIAALRKVAGERVAGRIRREP
                     DPFIERVVAGWPRNFAPARALELGFKADASFEDIIRIHIEDELGGRVA"
     gene            5469..5798
                     /locus_tag="VFR00_13480"
     CDS             5469..5798
                     /locus_tag="VFR00_13480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0160327.1"
                     /translation="MHKALAVSVAAIILALPTLAAAQTRGPRGPVEGVVVAESFYGHG
                     TATAPVRRNSKGRLEVGLPGGTWYECAFSCSDTLRRQTVDFWENHSGRPRDGGGDGVG
                     YFRFRWP"
     gene            complement(5815..>6141)
                     /locus_tag="VFR00_13485"
     CDS             complement(5815..>6141)
                     /locus_tag="VFR00_13485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEU0160328.1"
                     /translation="EAVRASMGSIFNVPVVRTEEKAFLAWRAHWRGEVVGTHLAAAED
                     FRRAAYRAPVLLLMGSEGPGLSPELVRAASRLVKIPMAGTLDSLNLAVATALLLYEIQ
                     RQRLQL"
BASE COUNT          953 a         2189 c         1965 g         1034 t
ORIGIN      
        1 cgctcgccga gcccagcctg atcggcatct cgggcggggc cgcgctcggg gccgtgctcg
       61 caattcatct cggcattacc caggcactgg cgctcgcgct gccgctgggc ggactgctgg
      121 gtgcgtttat cgccgtgctc gccgtggtgg cgctcgcggg cctcaacggc gggcccatca
      181 ccctcattct ggcaggcctc gccgtctcgg ccaccaccac cgccctcatc tcccttgccc
      241 tcaatctgtc acgcaacccc tttgcctcca tcgagatggt ttactggatg atgggctcgc
      301 tcgccgaccg cagcctgacg caggcctggc tcagcgggcc cttgatgctt atcggcatgg
      361 gcctctcgct tttgcttggc cgcgcgctcg atgccctctc gctgggggag gacgccgccg
      421 ttagcctcgg catcgatgcc cgccgcgccc ggctcacgct tgtggcgggc accgcgctca
      481 gcgtcggcgc cgccaccgcg gtcaccggca tcatcggctt tgtgggtttg ctggtgccgc
      541 acgcgctacg cccgctgacc gggcatcgtc ccggcctctt gctgccggcc agcctattcg
      601 gcggcgcgat cctggtgctc cttgccgacg tcgccctgcg gctcgtacag ccgtgggtcg
      661 atctacgcat cggcgtcttg accgccctgt tcggagcgcc attttttatt tggctggtgc
      721 tcaagacccg ccgcgagctg gcgccatgaa gctcgaagca cggggcttgc gggtccactt
      781 acggcaacgc gaggtgctaa ggggtgtcga cctggcggcc gcagccggtg agttgaccgc
      841 cgtgatcggg gccaatggcg ccggcaaaac cacgctgctt cggacgttga gcggtctgct
      901 gccgttcagc gccggcgagg cccggctcga tcaacgccca ctgccggcct ggccgcgccc
      961 cgaattggcg cgctgtctcg cctatctgcc acaggagcgc acggtccact gggcgctgtc
     1021 ggcgcgcgcc gtggtggcgc tcggccgcct accgcaccag acgctgggcc aaggcgaaac
     1081 cgttgccgat cgcgccgcca tcgcggcggc gatcgccgcc atggatattg acgcgctcat
     1141 cgctcgcccc gttttggaac tgtcgggtgg ggagagggcg cgcgtcctgt tggcgcgagc
     1201 gctggcacag gagccacagg tgctcgtcgc cgacgagccg gtcgccggcc tcgatcctgc
     1261 ccatcagctc gcactcttcc atcacttgcg agtgtttgcg gcgagcggcc gcagcgtcct
     1321 cgtcgcgctg cacgatctcg ctctcgccgc gcgcttttgc cacaagatcg tgctgctcaa
     1381 ggccggtgca gtcctgacgt caggaccacc gtcccaggtg ctgacgcgcg agcacctggc
     1441 ctccgcctac gatatccgcg ccgagtatcg cacaattgat ggcattgcga ctgtgctatc
     1501 gcgcgatggg ctgccatgat gccgccctgg ttcggagctt tggaccggcc cggagtttgc
     1561 cagtggcagg gggcaatcag tgctgcgact ttggcggcgg cagcggcgcg tcaatctggc
     1621 gcttgaaggc ggtggcgcac acggcgcctt cacctggggt gtgcttgatc ggctgctgga
     1681 ggaggatcag ctcgagatcg gctggctgag cggcaccagc gcgggcgcgg tcaatgccgt
     1741 cgcgctcgca gccggcttgg ccgagggcga tcgcacgcgc gcacgagcca agctgcgcga
     1801 ggtatgggaa gccgtcgcca aagccggcgt gccggatctg atgcggctca atccatggct
     1861 tgcctcgctg tcacgctccc agaccctggc gcaggtcgcc tcgctgttct cgccctatga
     1921 cttcaatccc ctgggcttcg atcccctgcg caaagtactc gaggctcata tcgacttcgc
     1981 cttgctgcgc acgcagccgg gacccgaact cctgatcgca gcgaccgacg ttcaaagcgg
     2041 ccggccgcgg ctgtttcgtc gccgcgagtt gaaggtggag tgcgtgcttg cctccgcctg
     2101 cttgccgacc ctgcatcatg cggtgtcggt ggacggccgt gcctactggg atggcggctt
     2161 caccgccaat cccgatctgg tcacgctcgc gcgcgaggcg cccgtcgccg ataccctgat
     2221 catcaaactc aatccgatgc tcgttgaaag cctccccacc agcgcacgcg agatcagcgc
     2281 ccgcatcaat cacatcacct tcaatcaacc gatgttgcgc gacgtcgaac tgatcgagag
     2341 cgtgcgtcgc aatcacaagg gctggccgcg gctgcggcgg gatgatgacc ggcggctcgc
     2401 ccgtcaccgc ttccatttga tcgaggccgg ccgcgtgacg ggcgcgctat cgccggaaag
     2461 caaggtcaaa cccgatctcg atcttctctt gcacctgtgc ggcgcgggcc gcagcgaaac
     2521 cgagaagtgg ttggcccggc acacccgagc catcggacgc cgcagcagcg tcgacctggc
     2581 gcaacatttt ctggcgcccc gcccctccac cgctgccgcc tccagcccgc ccagaccaaa
     2641 gccggaggcc gcggcctgac aacgccgttg ggatcggctt gacccccgac cgccggccgg
     2701 cccacgatag cgtccgccac ccacgcaagt gctgctgcag ctgagccaag gaaataccat
     2761 gaccgaccgt ctcaagggca agctcgcctt ctgcaccgct tccgccgccg gtatcggccg
     2821 tgccacggcg attgcctttg cgcgcgaggg cgcgcgcgtc atcgccagcg acatcaataa
     2881 ggcgggcatg gcgggcttga cggaggccgg cgtcgccgaa tgcgtcgcgc tcgatgcgcg
     2941 cgacgcagcg gccatcgagg cgcaagcgaa gcggctcggt gcggtcgacg tgctcttcaa
     3001 tgccgcaggg ttcgtgcacc atggcagcgt gctcgatgcc accgatcagg agtgggattt
     3061 cgccttcgac ctcaacgtca aatccatgca tcgcaccatc aaggccttcc taccggacat
     3121 gctgcaggcc ggccggggct ccatcgtcaa tatcgcctcc accgtcggcg cggccaaggc
     3181 ggcgcccaac cgctacatct acggagccag caaggccgcc gtggtgggcc tgaccaaggc
     3241 ggtggccatc gactttattg gcaagggtat ccgctgcaac tgcatctgcc cgggcaccat
     3301 tgcgacgccc tcgctcgacg agcgcatccg cgcccttggc cgtcagctcg gcagcgagga
     3361 gaaggcccgc gaaatgttca tcgcgcgcca acccatgggc cggctcggca ccgccgaaga
     3421 gatcgcctac gtcgccgtct atctcgcctc cgatgagtcg gcctatatga ccggctccgc
     3481 aatcattccc gacggcggct tcaccctgta atccctgaaa catcgtggag gtgaaacagt
     3541 atgccccgct ttgccgccaa tcttaacttc ctgtttaccg atcttccctt cctcgatcgc
     3601 ttcgacgccg ccgccaaggc cggcttcaag gccgtcgagt cgaccgacct ttattccgcc
     3661 acggccaagg aggtcgccgc ccggctgcgc gccaatgcgc tggaactcgc cctcttcaat
     3721 gtcaaccccg gcgaccccaa caagggcgag cgcggcgtgg cggcgctgcc ggggcgcgag
     3781 gccgatttcg ccgcccgctt cgagaccgcg ctcagctacg cggaggcaac cggcgggcgc
     3841 ctgatccacg tcatgccggg gcttgtgcac cagggcgcgc ggcgcgacgt ctacctcgcc
     3901 aacctcaaga aggcggcgcg ccaggccgcc gcggccggcg cccaaattgt gatcgagccc
     3961 atcaatcgtc gcgacattcc gggctacttt ttgaacaaga cgcatgaggc gcgcgccatc
     4021 atccatgagg tggccgaacc caacctcggc ctgcaattcg acctctatca tcgccaaatc
     4081 gaagaaggcg acgtggccat ggcgatcgat gagttcggcc cgctcgcgcg ccattatcag
     4141 atcgccagcc cgcccgatcg cggtgagccg gacgccggcg aaatgaacta tccttggctg
     4201 ttccagaaaa tcgacgccag cggcttcaaa ggctacatcg gctgcgaata ccgcccgcgc
     4261 ggcgacacgg cggcgggcct caaatgggcc gcggcctgcg gcgtgagttt gggctagggc
     4321 taggagctga gccatgaaca ttctgatcat cggcggcgcc ggcatgatcg gggccaagct
     4381 ggcgggacgc ctggcccggg acggcacgct caacggcaaa ccgatcacga gccttagcct
     4441 ttacgacgtc gtccccccgg ccgcgccggc ggcaaccaaa atgccggtca aggtcatgac
     4501 cggcgacctg ccgacgcccg gggaggccgg caaactgatg gcggaccggc cggacctcat
     4561 cttccacctg gcggccatcg tctccggcga ggccgaactc gagttcgaca agggctaccg
     4621 tatcaacctc gatggcacgc gcctcctact cgaggcgatc cgcgccgcgg gccacacccc
     4681 gcgcctcgtc tttaccagct cgattgccgt gttcggcgca ccgtttccgg aggcgatcgc
     4741 ggatgaattc ttcaatacgc cgctgacctc ctacggcacg caaaagacca tctgtgaact
     4801 gttgatctcc gactatacgc gcaagggctt cctcgacggg ctcagcattc gcttgcccac
     4861 catctgcgtg cgccccggca agcccaacaa ggcggcctcg ggcttctttt ccaatatcat
     4921 gcgcgagccg ctggccggcg aggccgccgt gctgccggtt tccgaggagg tgcgccactg
     4981 gcatgcctcg ccccgcgccg cggttggctt cctgctccac gccgcgagcc tcgataccag
     5041 cacggttggc ccccgccgcg cgctgaccat gccgggcgtc tcggtcaccg taggcgagca
     5101 aatcgccgcg ctcaggaagg tcgcaggcga gcgggtggca gggcgcatcc gccgcgagcc
     5161 cgatcccttt atcgaacgcg tggtggcggg ctggccgcgc aattttgcgc cggcgcgcgc
     5221 gctcgagctg ggcttcaagg ccgatgccag cttcgaagac atcatccgca tccacatcga
     5281 ggatgagctc ggcggccgcg tcgcctgagg ccggccgtct tgccggtgtg accggctggc
     5341 tacaggccgc cggcgatccg ggcgccgggg gtgcggcgtc cgcgatttga ccacgaactc
     5401 ttgtaggctg tggccctgcc gcgcgggcgc tcgcaccgca gccgccgcga acgggagtga
     5461 ggggatatat gcacaaggct ctcgccgtca gcgttgccgc gatcattctg gccttgccca
     5521 cgcttgccgc cgcccagaca aggggcccgc gcggtccggt cgaaggcgtg gtggtggcgg
     5581 agagctttta cgggcacggc acggcgaccg cgccggtccg gcgcaacagc aaggggcgcc
     5641 tggaagtcgg cctgcccggc ggcacctggt atgagtgcgc cttcagttgc agtgacacct
     5701 tgcgtcggca aaccgtcgac ttctgggaaa accacagcgg ccggccgcgc gacggcggcg
     5761 gcgacggcgt cggctacttc cgcttccgct ggccctgagg cgcgcgccgc accctcacag
     5821 ctgcaggcgc tggcgctgaa tttcatagag cagcagcgcc gtcgcaactg ccagattgag
     5881 cgaatcgagc gtgccggcca tgggaatttt gacgagacgc gaggcggcac gcaccagctc
     5941 cggcgacagg cccggtcctt cgctccccat cagcaacaag accggcgccc ggtaggccgc
     6001 ccggcggaag tcctcggccg ccgccagatg cgtgccgacc acctcgccac gccagtgcgc
     6061 gcgccaggcc aggaacgctt tttcctccgt gcgcaccacg ggcacgttga aaatcgagcc
     6121 catgctggcg cgtaccgcct c
//
LOCUS       DASTMT010000591         4219 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_5460262, whole genome shotgun sequence.
ACCESSION   DASTMT010000591 DASTMT010000000
VERSION     DASTMT010000591.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4219)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4219)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4219
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5460262"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..188)
                     /locus_tag="VFR00_13490"
     CDS             complement(<1..188)
                     /locus_tag="VFR00_13490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011088452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEU0160329.1"
                     /translation="MTKRIQAKHKIDRRLGQNIWGRPKSPINKRESRPGQHGERRSGK
                     LSDYGQQLRAKQKLKGYYA"
     gene            complement(435..1055)
                     /locus_tag="VFR00_13495"
     CDS             complement(435..1055)
                     /locus_tag="VFR00_13495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0160330.1"
                     /translation="MIGKGARAGIGAVLALAAWGGPALAQNKELSDKSVLTLMQYAWS
                     LVPNKFTSPDGKVIEVDKSKPSEVVVPMDTAREAIKVARLSAYAQVCDLIEEQRANYH
                     TFMKREEAKGKWSDQQLLFINQLHLFTVMTLTGKVKLVEKDGDKEVILNSDKPEKAPT
                     CTETERKKVETQIKSYVAANVAPAAPEPAAPATTGATSVRPASQKK"
     gene            complement(1166..2404)
                     /locus_tag="VFR00_13500"
     CDS             complement(1166..2404)
                     /locus_tag="VFR00_13500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEU0160331.1"
                     /translation="MKDVVVSAGEPRLAGTELAVGAVIERAYQTSMHAGLGQLRIAGL
                     ERAGLEAVLSYCAEQRCRGDQASCPGCRLTTERLGLKTFDDFVARFAEVTFRHSDLRL
                     VGAGSERLEAESLQALAKTWAGEEYWFWARRLLRKLRHGVRRAHLSGAPPEPQPTPAL
                     VLVRPQLADNIGMVARAMANFGLEELRLVAPRDGWPNEKARIAASGANFVIDAATAYP
                     SLTAALAGVHWVAATSARQRDLAKPVMTPEQAVAEIAQRIAAGQRCAILFGPERNGLE
                     TEEIANADALIMAPVNPAFASLNLAQAALLMSYEWMKRAGHGTLGRVTTYEQPVEAGL
                     RLRGSQPAGREELIGFFEHLERELDTTGFFSSPEKRPSMVRNMRTMFTRMGATQQEIR
                     TLRGIVKALVNGRRTTRKAP"
     gene            complement(2515..3402)
                     /locus_tag="VFR00_13505"
     CDS             complement(2515..3402)
                     /locus_tag="VFR00_13505"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEU0160332.1"
                     /translation="MRQLTVHVNGIDLCLRATGAPTAPLVLFLHGFPEYSGAWDEVLP
                     AFADRFHAVAPDQRGYAHSGKPENLEAYRIKYLVRDILGLGERLSPGRPFSIVGHDWG
                     ASVAYATAIAAPARVARLVVLNGVHPGPFQRALLEDAGQRAASAYIHDLRDQSAEARL
                     SANRFEKLLALLRRFGGKAWLTPAKEAGYLAAWSVPGALTAMLNWYRATPLLVPKVGE
                     TVDVGALAKLDPATLRVRMPHLLIWGMQDSALLPVSWARLPDYCDDLVVREIGDGDHW
                     LVHQKTEEVTRLIGGFLGG"
     gene            3552..>4219
                     /locus_tag="VFR00_13510"
     CDS             3552..>4219
                     /locus_tag="VFR00_13510"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009736907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006102 - isocitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HEU0160333.1"
                     /translation="MQKIKVTGTVVEMDGDEMTRIIWQLIKDKLIHPYLDLNLEYYDL
                     GIESRDKTNDQVTIDAANATKRHGVAVKCATITPDEARVKEFNLKEMWKSPNGTIRNI
                     LGGVIFREPILCTNVPRLVPGWTQPFVIGRHAYADQYRATDFKFPGKGTITLKFVGDD
                     GKVIEREVFKSPGAGVTMAMYNLDDSIRDFARASFNYGLARNYPVYLSTKNTILKVYD
                     GRFK"
BASE COUNT          725 a         1412 c         1332 g          750 t
ORIGIN      
        1 gcgtagtagc ccttcagctt ctgctttgcc ctgagctgct ggccgtagtc ggagagcttg
       61 ccgctgcggc gttcgccgtg ctggccaggt cggctctcgc gcttgttgat cgggcttttt
      121 ggccgccccc agatgttctg gcccaggcgc cgatcgatct tgtgcttcgc ctggatgcgc
      181 ttggtcatgc tctagtctcc aaagtcgtgt aacaatcgtg aacgcgaata gaacgcacgc
      241 cctcctctac ccggcatcga ccggatcgac aggccccccg agagttgcgg gttgccacgg
      301 atgtgcaaac gggaagggcg gaccgcatgg gcccgccgca gcagccttat atagcacggg
      361 cgggccaggt caatcgcccg gcccgggcga ccggccgcgg ggccgcgcga cctgcaggcg
      421 aaggtttatt gggcctattt cttttggctg gcaggcctga ccgacgtggc gccggtggtg
      481 gctggggccg cgggctcggg tgccgccggc gccacattgg cggcgacata ggacttgatc
      541 tgagtttcga ctttcttgcg ctccgtctcg gtgcaggtcg gcgccttttc cggcttatcg
      601 ctgttgagga tgacctcctt gtcgccgtcc ttctcgacca gcttgacctt gcccgtcagc
      661 gtcatgacgg taaacaggtg cagctgattg atgaacagga gctgctggtc gctccacttg
      721 cccttcgctt cctcgcgctt catgaaggtg tgatagttgg cccgctgctc ttcgatcaaa
      781 tcgcacacct gggcataggc cgacagccgg gccaccttga tggcctcgcg cgccgtgtcc
      841 atggggacga cgacctcact cggcttgctc ttgtcgacct cgatcacctt gccgtcgggg
      901 ctggtaaatt tgttgggcac caaagaccag gcgtactgca ttagggtcag tacggactta
      961 tcgctcagct ccttgttctg agccaacgcc ggcccgcccc acgccgccag cgccaacacc
     1021 gccccaatgc cggcccttgc acctttgccg atcattctgc agcccctgtt tgtccagccc
     1081 aattgcaacg ttctcatccc gttcgcgggc ggcagtccca ctctgcctag cgcgtgcgca
     1141 tcgctcaacg ggaatcgagg cataactaag gcgcttttcg agtcgtgcgc cgcccgttca
     1201 ccagggcctt gacgattccc cgcaaggtgc ggatttcctg ctgcgttgca cccatccgcg
     1261 taaacatggt gcgcatatta cgcaccatgc tggggcgctt ttcgggcgaa gagaaaaagc
     1321 cggtcgtgtc cagctcgcgc tcgagatgct cgaaaaaacc gatcaactcc tcgcggccgg
     1381 ccggctggga gccacgcagg cgcaggccgg cctcgacggg ctgctcgtag gttgtgaccc
     1441 gccccaaggt gccgtgcccg gcccgtttca tccactcgta gctcatcagc aacgcggcct
     1501 gggccaaatt gagcgaggca aaggctggat tgacgggcgc catgattaag gcatccgcgt
     1561 ttgcgatctc ttccgtctcg agaccattgc gttccggccc gaacaggatg gcgcagcgct
     1621 gcccggcggc gatgcgctgg gcgatctcgg ccacggcctg ctccggggtc atgaccggtt
     1681 tggcgagatc gcgctgtcgg gcggacgtgg ccgccaccca gtgaacgccg gccagtgcgg
     1741 cggtcaggga ggggtaggcg gtggccgcat cgatcacgaa attggcgccc gaggcggcaa
     1801 tgcgcgcctt ttcattgggc cagccgtcgc gcggggccac caggcgcaat tcctctagcc
     1861 cgaagttggc catggcgcgg gccaccatgc cgatattgtc ggccaactgg ggacgcacca
     1921 ggaccagcgc aggggtgggc tgaggctccg gtggggcgcc gctgaggtgg gcccggcgca
     1981 ccccgtggcg cagcttgcgc aagagccggc gggcccagaa ccaatattcc tcccctgccc
     2041 acgtcttggc caaagcctgc aggctttccg cttctagccg ctcgctaccg gcaccgacga
     2101 ggcgcaggtc ggagtgccga aacgtcacct cggcaaagcg ggccacgaaa tcatcgaagg
     2161 ttttcaggcc gagccgctcg gtggtgagcc ggcatcccgg gcagctcgcc tgatcgccgc
     2221 ggcagcgctg ctcggcgcaa tagctgagga cggcctccag tcccgcccgc tcgaggccgg
     2281 caatccgtag ctggccgagc ccggcatgca tgctcgtctg gtaggcgcgc tcgatcacgg
     2341 cgccaaccgc caactcggtt ccggcaaggc gcggttctcc cgcgctgacc accacgtcct
     2401 tcacttttgc ctctggggct taagctctca gcgttgctgt ctggcttggc caatgttagc
     2461 gggcgcgggg cccgaatgca ggcacgaatg cacgtggggg gcgcgaggcc cacctcagcc
     2521 ccccaaaaac ccaccgatca gccgggtcac ctcttcggtc ttctgatgca ccagccaatg
     2581 gtcgccatcg ccaatctcgc gcaccacgag gtcgtcgcag tagtccggca gcctcgccca
     2641 ggacaccggc aacagcgcgc tatcctgcat accccagatc agcaagtgcg gcatgcgcac
     2701 gcgcaaggtt gccggatcga gcttggcgag ggcgccgacg tcgacggtct cgccgacctt
     2761 cggcaccagc agcggggttg ctcggtacca gttgagcatg gcggtcagcg cccccggaac
     2821 cgaccaagcc gccagataac cggcctcctt ggcgggcgtc agccaggcct tgccaccgaa
     2881 ccgacgcagc aacgccagta gcttctcgaa ccgattggcc gataggcgcg cctccgcgct
     2941 ctgatcgcgc aggtcatgga tataggcact ggcagcgcgc tgcccggcat cctctaggag
     3001 cgcgcgctgg aagggccctg ggtgcacgcc gttgaggacc accagccgcg ccacgcgcgc
     3061 gggcgccgca atcgccgtcg cgtacgcgac cgaggcgccc caatcgtgcc ccacgatgct
     3121 gaacggccgg cctggcgaca gcctctcgcc aagccccagg atgtcgcgca ccaggtactt
     3181 gatgcggtag gcctcgagat tttcgggctt gccggagtgg gcatagccgc gctggtcggg
     3241 ggccacggcg tggaaccggt cggcgaaagc cggcagcacc tcgtcccagg cgccggagta
     3301 ctccggaaag ccgtgcagga acaggactag gggggccgtg ggcgcgcctg tggcgcgcag
     3361 gcaaaggtcg atgccattga cgtgaactgt gagctggcgc atgcggggct cccggccagc
     3421 cgcaagcctg cccaaaagcc taggcgctgt cgagagttgt cggccgcctg cggcgccagc
     3481 aacctttgcc tgcggccgcc cgggtgctaa gagggcggcg ctttggcgcg acgcgattgg
     3541 agggagcttc catgcagaag atcaaggtca ccggcaccgt cgtcgagatg gacggcgatg
     3601 agatgacgcg gatcatctgg cagctcatca aggacaagct gatccaccct tatctcgacc
     3661 tgaacctgga atattacgac ctcggcatcg aaagccgcga caagaccaac gaccaagtca
     3721 ccatcgatgc ggccaacgcc accaaacggc acggcgtggc ggtcaagtgc gccaccatca
     3781 cgccggacga ggcacgcgtc aaagagttca atctcaagga aatgtggaaa tcgccgaacg
     3841 ggacgatccg caatattttg ggcggcgtca ttttccgcga acccattctt tgcacgaacg
     3901 tgccgcggct ggtacctggc tggacccagc cgttcgtgat cggccgccat gcctatgccg
     3961 accagtaccg cgccaccgat ttcaagtttc cgggcaaggg caccatcacc ctgaaattcg
     4021 tcggcgacga cggcaaggtg atcgaacgcg aggtgttcaa gtcgcccggc gccggcgtca
     4081 ccatggcaat gtacaacctc gacgattcca tccgcgattt cgcccgtgcc tccttcaact
     4141 acggcctggc ccgcaattat cccgtttatc tttcgaccaa gaacacgatc ctcaaggtct
     4201 acgacggccg cttcaagga
//
LOCUS       DASTMT010000592         2388 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_5461808, whole genome shotgun sequence.
ACCESSION   DASTMT010000592 DASTMT010000000
VERSION     DASTMT010000592.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2388)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2388)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2388
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5461808"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(245..532)
                     /locus_tag="VFR00_13515"
     CDS             complement(245..532)
                     /locus_tag="VFR00_13515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0160334.1"
                     /translation="MKRERPADDIRRRAIKARLQGMPSREIARRLERSHDTIKGYLGG
                     CGATIKGTYYLNALAAEALFSEHELTDMGAIWNGRCWVGPGRIHRGGAAAI"
     gene            complement(534..1451)
                     /locus_tag="VFR00_13520"
     CDS             complement(534..1451)
                     /locus_tag="VFR00_13520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0160335.1"
                     /translation="MQDPDDGRVPLERAVLRTAIGNYPHTAALRDGRISSERLSLDFV
                     ELAPINRAFAPMLRDGRFDVSEIAIASFLQAKAYDKPFVLLPVVLAARFQEPALLCLT
                     ASTIRSPVDLLGRRIGGRAYSQTTGVWLRGILADAAGVPPQAVRWVTFEDAHVAEYRD
                     PPWVERAPVGADLMGMLRQGALDAVIVGNDVPADPTLRTVFPDPAAAADAFWHRHGVM
                     PINHMVTVGSDLAERRPDLVRELIGMFRAAAAAAPGTGKAAHALDATALTCALQLALR
                     YATEQGLLPRALSVAELWAGPSVAEAPRD"
     gene            complement(1455..2144)
                     /locus_tag="VFR00_13525"
     CDS             complement(1455..2144)
                     /locus_tag="VFR00_13525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010969451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047443 -
                     4-hydroxy-4-methyl-2-oxoglutarate aldolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-carboxy-4-hydroxy-2-oxoadipate
                     aldolase/oxaloacetate decarboxylase"
                     /protein_id="HEU0160336.1"
                     /translation="MSLVITNPPRVGRTLSEAFARYGVATVHEAQARRGLLAASMRPI
                     YAGAKIAGSAITVSIPPGDNWMLHVVVEQCQAGDVLVVAPTSPCDNGYFGELLACSLV
                     ARGVLGLVIEAGVRDVAALTQMRFPVWSRAISAQGTVKETLGSVNVPIVCAGQLIHPG
                     DLILADDDGIVVVPGGSAEIVLGQSQAREAKETQTRARLQAGELGLDIYGMRSKLKEK
                     GLAYRPTDMEG"
     gene            complement(2141..>2388)
                     /locus_tag="VFR00_13530"
     CDS             complement(2141..>2388)
                     /locus_tag="VFR00_13530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010969450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PIG-L domain-containing protein"
                     /protein_id="HEU0160337.1"
                     /translation="LFEPHQPEQCDWNPTVLLDISAVWNKKRAAIECMAGQEHLWEYY
                     TRVGLQRGAQAARNSDKKITYGEGYESVFPHVLEALA"
BASE COUNT          398 a          779 c          824 g          387 t
ORIGIN      
        1 aacgcccgag atctttcccg aagccgatcc gaggatcccg cgcgtcggca acagcggtag
       61 cggcggcagc ggctaggtct tggcaaagga aatccgtggc ccctcgtggc agtcgcgcct
      121 tcgccgcggc catggcagcg ccggcggctt gattgccgcg cggctgaagg tgccggcccc
      181 agccgcgtcg gcgttgcggg cgcgaggcaa ttgccggcca agcggggcgt tgcccttgcg
      241 gccactagat cgcggcggca ccgccgcggt ggatgcgacc tggcccgacc caacagcggc
      301 cattccaaat ggcgcccatg tccgtcaact cgtgttcgct gaacaaggct tcggcggcta
      361 gggcattcaa atagtaggtt cccttgatgg tggcgccgca gccgccaaga tagcccttga
      421 tggtgtcgtg gctccgctcg agacgccggg cgatttcgcg gctgggcatg ccctgtaggc
      481 gtgccttgat ggcccgacgc ctgatgtcgt cggcaggacg ctcgcgcttc atgctagtct
      541 cgcggagcct ccgcaacgga tggtccggcc cagagctccg cgaccgacaa ggcgcgcggc
      601 agcagaccct gttcggtggc gtagcggagc gccagctgaa gcgcgcacgt gagcgccgtc
      661 gcatcgagcg catgggctgc cttgccggtg ccgggggccg cggcggcggc agcccggaac
      721 atgcctataa gctcgcgcac gagatccggg cggcgctctg ccaaatcgct ccccacggtc
      781 accatgtgat tgatcggcat gacgccgtgc ctgtgccaaa acgcgtccgc ggcggcggcc
      841 ggatcgggga agaccgtgcg cagtgtggga tcggcaggca cgtcgttgcc gacgatgacg
      901 gcgtccaagg cgccttgccg caacatgccc atcaggtcgg cgccaactgg ggcccgctcg
      961 acccagggtg ggtcgcggta ctcggcgacg tgcgcatcct caaaggtgac ccaacgcacc
     1021 gcctggggtg gcacccccgc agcatccgcc aggatgccgc gcagccagac accggtcgtt
     1081 tggctatagg cgcgcccgcc gatgcggcga ccgaggagat cgacggggct acggatggta
     1141 ctggcggtga ggcacaaaag cgcgggctcc tgaaagcggg cggccaacac cacgggaagc
     1201 aagacaaaag gtttgtcgta ggccttggct tgcaggaagc tggcgatggc gatttcgctg
     1261 acatcgaagc ggccgtcgcg cagcatcggc gcgaaggcac ggttgatcgg agcaagctcg
     1321 acaaaatcaa ggcttaagcg ctcggacgag atgcggccgt cgcgcagggc ggccgtgtgc
     1381 ggatagttgc cgatggcggt gcgaagcacg gcacgctcga gcggcacgcg gccgtcgtcc
     1441 gggtcctgca tgcctcagcc ctccatgtcc gtcgggcgat aggcgagacc tttttctttc
     1501 aacttcgagc gcatgccgta gatgtctagg ccgagctcgc cggcctgcag gcgcgcccgg
     1561 gtttgggtct ctttggcctc acgggcttgg ctctggccaa gcacgatctc agccgagccg
     1621 cctggcacca cgacgatgcc gtcgtcatcg gcgaggatca gatcgccggg gtggatgagc
     1681 tgcccggcac agacgatcgg tacgtttacc gagcccagcg tctccttgac ggtgccttgc
     1741 gccgagatgg cccgtgacca caccggaaag cgcatctgcg tcagggccgc aacgtcgcgc
     1801 acgccggcct cgatgaccag ccccagcacc ccgcgcgcga ccagcgagca ggcgagaagt
     1861 tcaccgaaat aaccgttatc gcagggcgag gtgggtgcca ccacaagcac gtcgccggcc
     1921 tgacactgct ccacgacgac gtgcagcatc cagttgtcgc cgggtggaat ggagacggtg
     1981 atggcgctgc cggcaatctt ggcgcccgca tagatcggcc gcattgaggc ggcaagcagc
     2041 cccctgcggg cctgcgcctc gtgtacagtg gcaaccccgt agcgcgcgaa ggcctcgctt
     2101 agtgtgcgtc cgacgcgtgg tggattggtg atgacgaggc tcatgccagc gcctccagca
     2161 cgtggggaaa gacgctctcg tagccctcgc cgtaggtgat ctttttgtcg gagttgcgcg
     2221 ccgcctgggc gccgcgctgc aggccaacgc gggtgtagta ctcccatagg tgctcctggc
     2281 ccgccatgca ttcgatggcc gcgcgcttct tgttccagac cgcgctgatg tccaacagca
     2341 ccgttggatt ccagtcgcat tgctcgggct ggtgaggctc gaacagaa
//
LOCUS       DASTMT010000593         5674 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_4806021, whole genome shotgun sequence.
ACCESSION   DASTMT010000593 DASTMT010000000
VERSION     DASTMT010000593.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 5674)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5674)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5674
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4806021"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(76..963)
                     /locus_tag="VFR00_13535"
     CDS             complement(76..963)
                     /locus_tag="VFR00_13535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011578918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEU0160338.1"
                     /translation="MWAYFINLAVLISINAILAVTLNFILGYAGIFSIAHALFFGVGA
                     YTATFVAMKFGAGFLTATAAGMLLAALISLALALPALRVRGEYFVAASLGLQMLAVTV
                     FTEWKSVTGGIGGLTNIPPARLFGFAVTQPEHFLLLSLLSLGLIILVIRALVRSSFGR
                     SLKAIRDDETAAWAYGKNVAIIKTTAVVVSSALAAVAGSLYAFYLSFINVESFVLDTS
                     IQLMAMVIIGGTATLLGPLIGTVLILLLPAALSYLPYLPVTEIGSIQQIVYGLAMVLL
                     MIYRPGGLWGFQDKPKADA"
     gene            complement(974..1849)
                     /locus_tag="VFR00_13540"
     CDS             complement(974..1849)
                     /locus_tag="VFR00_13540"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEU0160339.1"
                     /translation="MLLLQLLINGLQLGAIYALTAVGFSLIFGATKIFHVAHGAAFTI
                     AAYLFWWATAIKGWPWPLAAGLAVLAAVVFGLAMERFIYRPIQRHEGAFFTVFIAAFG
                     VQIIVQNLIGTVFGRGFVAVTTPLSRSLEVLPGLFVAPVAALAIVFAVVGFTGLTIYL
                     ARTHTGMAMRALSENPDLIRVFGLDPGRVAQYAFAIGSAIVVPGAIFTAMTAGLNPAV
                     GAHVMLISLAATIVGGIGSLRGAAYGGLLLGLAENLALWRLDPQWSEAVTFVVLFLFI
                     IFRPAGFFGRPHAAK"
     gene            complement(1876..3003)
                     /locus_tag="VFR00_13545"
     CDS             complement(1876..3003)
                     /locus_tag="VFR00_13545"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0160340.1"
                     /translation="MRLVRLLALLAAFQLAAATARAQDSIKIGADLPMSGPNAEYGEL
                     FGAAANLAVEHANADKMLSKKLLIQFEDSQATPQQGVVAMNKLVNVEKVPFVLSAFTG
                     VSKAIAPVGDRAKVVSVNGGGIGPDLAELGEYFWNVIPLVNLEIRVMAPYLVQERGFK
                     KIALVYVDDPAGEAIQKELKDNVPKAGGQMVAELQVPRNAQQFTSIAAKVREAGPDLV
                     FIASFGAQQAQIVKQLRDNGVSQQLASYSAFSSPTVTQLPEAKGALFPTQSVDWAAPG
                     MSARVAKDWKAKTGKEPNAYVANYYNAVMVFVHLAQALEKKGQPITGENLLQQRKANG
                     SFELVGGKMEFLPNGSVSMPIQINEIDGGAGKLVKRGTLAK"
     gene            complement(3311..4330)
                     /gene="ilvC"
                     /locus_tag="VFR00_13550"
     CDS             complement(3311..4330)
                     /gene="ilvC"
                     /locus_tag="VFR00_13550"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018319725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEU0160341.1"
                     /translation="MRVYYDRDADINLIKGKKVAVIGYGSQGHAHALNLRDSGVKNVA
                     IALRKTSAGVKKAEAEKLKVMEVAEAAKWADVMMMLTPDELQADIYKDHLRDNMKDGA
                     ALMFAHGLNVHFNLIEPRADLDVGMIAPKGPGHTVRSEYLRGAGVPCLLAIQQDKSGN
                     AHDLFLSYGAAIGGGRAGIIETSFKEECETDLFGEQAVLCGGLVELIRAGYETLVEAG
                     YAPEMAYFECLHEVKLIVDLIYEGGIANMNYSISNTAEFGEYVSGPRVITEDTKAEMK
                     RILRDIQSGQFASMWMQEFRSGLPRFKATRRNNDAHPIEEVGAKLRAMMPWIKERALV
                     DKAKN"
     gene            5098..>5674
                     /locus_tag="VFR00_13555"
     CDS             5098..>5674
                     /locus_tag="VFR00_13555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008970016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HEU0160342.1"
                     /translation="MQRFVRSMFPSALGALLLAVALHALAPAARAQSGEGWTTLLDDK
                     TMGEWNRVGESNWRHEDGAVIADKKSGQDPGYLVSKSPYKDFQLHVEFWASDDANSGV
                     FLRCQDPSKLGDRSCYEVNIFDQRKDPTYGTGGIVNFSEVNPMPKAGGKWNTFEITAK
                     GRLITVVLNGQKTVELHNGMFVEGPLGLQHGQ"
BASE COUNT         1084 a         1787 c         1843 g          960 t
ORIGIN      
        1 cgtcgtccac cgccttgagg ccgccgaacg cgcgcgtggc gttcttcacc tgcagcagga
       61 tgtcctgttc gccgttcatg cgtccgcctt cggcttgtcc tggaagcccc acaggccgcc
      121 ggggcggtag atcatcaaca gcaccatggc gagcccatag acgatctgtt ggatggagcc
      181 gatctcggtg acgggcagat agggcaaata gctcaacgcc gccggcagca acaggatgag
      241 aacggtgccg atcagcggac ccaacaaggt ggccgtgccg ccgatgatga ccattgccat
      301 cagttgaatc gaggtgtcga gcacgaaact ctcgacattg atgaaactca aatagaaggc
      361 ataaagcgag cccgccaccg ccgccaaggc cgaggacacg acgacggcgg tggtcttgat
      421 gatggccacg ttcttgccat aggcccaggc cgccgtctcg tcgtcgcgga tggcctttaa
      481 gctgcggccg aagctggagc ggaccagggc tcggatcacg aggatgatca gcccgagcga
      541 gagcaaggac aacagcagaa agtgctcggg ctgcgtcacg gcaaagccga acaggcgggc
      601 gggtggaatg ttggtcagcc caccgatgcc gccggtgacc gacttccatt cggtaaacac
      661 cgtcacggcc aacatctgca ggccgagcga agcggcgacg aaatattccc cacgcacgcg
      721 caaggcaggc aacgccagcg ccaaggagat gagggcggca agcagcatac cggccgccgt
      781 cgccgtcagg aaaccggcac cgaatttcat ggcaacgaag gtcgccgtgt aggcaccgac
      841 cccaaagaac aaggcgtgtg caatggaaaa gatgccggcg tagcccaaaa tgaaattgag
      901 cgtgacggca agaatggcat tgatggagat cagcaccgcc aaattgataa agtatgccca
      961 catgccccgc gcctcacttg gcagcatggg gccggccgaa gaagcccgcc ggccggaaga
     1021 tgatgaaaag gaagagcacg acgaatgtca ctgcctcgct ccattgcgga tcgagccgcc
     1081 acagggctaa attttccgca aggcccagca atagtccgcc ataagcggcg ccgcgcaacg
     1141 agccgatgcc gccgacgatg gtggctgcca ggctgatcag catcacgtgc gcaccgaccg
     1201 ccgggttcag tcccgccgtc atggctgtga agatcgcgcc cggcacgacg atcgcggagc
     1261 cgatagcgaa ggcgtattgt gccacgcgcc ccggatcgag gccgaagacg cggatcagat
     1321 ccgggttttc cgagagcgcg cgcatcgcca tgcccgtatg cgtgcgcgcg agatagatgg
     1381 tcaagccggt gaagccgacg acggcgaaca cgatggccaa cgccgccacc ggtgccacga
     1441 acaggccggg caggacctcg aggctgcggc tgagcggcgt cgtcaccgca acgaagccgc
     1501 gcccgaacac cgtgccgatc aggttctgca cgatgatctg cacgccgaag gcggcgatga
     1561 agacggtaaa gaatgcccct tcatgccgtt ggatcgggcg ataaatgaag cgctccatgg
     1621 caagcccgaa gacaaccgcc gccagcaccg ccaggccggc cgcaagcggc caaggccagc
     1681 cttttatcgc cgtggcccac cagaacagat aggcggcaat ggtgaaggcc gccccgtgtg
     1741 caacgtgaaa gattttggtg gcgccgaaga tcagcgagaa gccgaccgcg gtcagcgcgt
     1801 agatagcccc gagctgaagg ccgttaatga gaagctggag cagcagcacg aggcaaaacc
     1861 gctagaagta cgccgttact tggccaaggt cccgcgcttg accaacttgc cggcgccgcc
     1921 gtcaatttca ttgatctgga tcggcatcga taccgagccg ttggggagga actccatctt
     1981 gccgccaacg agctcgaaac tgccgtttgc cttgcgctgc tgcaacaggt tctcgccggt
     2041 aatcggctga ccctttttct ccaacgcctg cgccaggtgc acgaacacca tcacggcatt
     2101 gtagtagttg gcaacatagg cgttgggctc cttgccggtc ttggccttcc agtctttggc
     2161 gacccgcgcg ctcatgccgg gcgctgccca atccaccgac tgtgtgggga agagtgcgcc
     2221 cttggcctcc ggcagctgcg tcaccgtcgg ggaggagaat gccgaatagc tcgcaagctg
     2281 ttggctgacg ccgttgtcgc gcagctgctt gacgatttgc gcctgctggg cgccgaagga
     2341 ggcgataaag accagatcag ggccggcttc gcgtaccttg gcggcgatgc tggtgaactg
     2401 ttgggcgttg cgggggacct gcagctcggc caccatctgc ccgcccgcct tcggcacgtt
     2461 gtccttgagt tccttctgaa tggcttcgcc ggccggatcg tcgacgtaga ccagcgcaat
     2521 cttcttgaag ccgcgctcct gcacgaggta gggcgccatc acgcggatct cgaggttgac
     2581 cagcgggatc acgttccaga aatactcgcc gagctcggcc aggtcgggcc cgatgccgcc
     2641 gccattgacg ctcaccacct tggccctgtc gccgaccggg gcgatcgctt tggagacgcc
     2701 ggtaaaggcc gacagcacga agggcacctt ttccacattg accagtttgt tcatggccac
     2761 caccccttgc tgcggggtgg cctggctgtc ctcgaactgg atcaaaagct tcttggacag
     2821 catcttgtcg gcgttggcat gttcaacggc gagattggcg gcggcgccaa acaattcgcc
     2881 gtactccgca ttgggcccgc tcatcggcag gtcggcgccg atcttgatgc tgtcctgggc
     2941 gcgagcggtg gccgcagcca gctggaaggc ggcgagcaga gccaagaggc gaacaaggcg
     3001 catgcgtttc ccctgctaga gagcgttttt tgcgtgttgt ttgagagacc aagatggata
     3061 ggcgatgaaa gcataccaat ccatgcctgg caatgtcgcc gcctgggcgc cgggctcgcc
     3121 cctggcatgc tgcaattgga ctggtgatcg atcggctttt gtgatcgggc ggcggcacgc
     3181 gggctcctgc cgtcgccctg cccccagcgg cgcggccgcg caggcggctg cggcgggggt
     3241 tcgcggcccg caacccttgc ggggcccagg cgccaacggc acctcgagcg gtgccgccgc
     3301 ggcgcttcag ctagtttttg gccttgtcga ccagcgcgcg ctccttgatc cagggcatca
     3361 tggcgcgcag tttcgccccc acttcctcga tcggatgggc gtcgttgtta cgccgcgtcg
     3421 ccttgaagcg gggcaggccg gagcgaaact cttgcatcca cattgaggca aattggccgg
     3481 attggatgtc gcgcagaatg cgcttcattt ccgccttggt gtcttcggtg atgacacggg
     3541 ggcccgatac gtattcgccg aactcggcag tgttggagat cgaatagttc atgttggcga
     3601 tgccgccctc gtagatgagg tcgacgatca gcttcacctc gtgcagacac tcgaagtagg
     3661 ccatttccgg ggcgtaaccg gcctccacca acgtctcgta acctgcccgg atcagctcga
     3721 ccagaccgcc gcagaggacg gcctgctcgc caaacaggtc ggtttcgcat tcctccttga
     3781 aggaggtctc gatgatgccg gcccgtccac cgccgatggc ggcgccgtag gacagaaaga
     3841 gatcgtgcgc attgccgctc ttgtcttgct gaatggcgag caggcatggc acgccggcgc
     3901 cgcgcaggta ttccgagcgc accgtgtggc ccggcccctt cggcgcgatc atgccgacgt
     3961 cgagatcggc gcggggctcg atcaaattga aatgcacgtt gaggccatgc gcgaacatca
     4021 acgccgcccc gtccttcatg ttgtcccgca ggtggtcctt gtagatgtcg gcctgcagct
     4081 cgtcgggggt cagcatcatc atgacgtcgg cccacttggc ggcctcggcg acctccatca
     4141 ccttgagctt ctcggcctcc gccttcttta ccccggccga ggtcttgcgc agggctatgg
     4201 cgacgttctt gacgccgctg tcgcgcagat tgagggcgtg ggcatggccc tgactgccgt
     4261 agccgatgac ggcgactttc ttgcccttga taaggttaat gtcggcatcc cgatcgtagt
     4321 agacgcgcat gggttcgctc cccggactga cctgactggc aaaactcctc ccctgctagc
     4381 ggctttcggc gccggcctgc aagcgcaaaa gcgccgcgcg ctctagggcg acggcccgca
     4441 cgcgccgcag gtcggcggca ggcgcagcgt ttggcaccgc cgcgaggtcg gcgtgcggaa
     4501 agcctccgac cggcaccgct gcggccgatt gcgcccgcga cgcgccggct aggacagggg
     4561 tcgatagcgt tgccgccggg ccggcgccga gcgccccttg gcgccgcgcc gggcgagctg
     4621 cgctggcgaa ggcccgcgag aggggggtgt ccaagcactg caagcccagc cgaggggtgc
     4681 gagcgtcggc acaatcccga gaccggtgcg cgcctgcgca agctcatgga cggtgagcgg
     4741 cgaggcctca gcccacgaat cccccagcac acgcgacagg ggcgagccca agggggccaa
     4801 tcaagtgcca acagggtccg cctccaacag gcgtgccgcc ctggcgcggt gcggccggcg
     4861 gcgcgctgca ggaaatcgca acgggccgtt cagccagcga ggggcagggt cggcagcttt
     4921 gggcgtgcct ctgccgcctg cgacttccgg ggcgcgggga acgcaactac aatgccggcg
     4981 gcgtgacggc atcgaaggtg cgcacttggg gttgatcagg tgccgcgcgc tgaccccgcg
     5041 tcgacagcgc ggtcatttgc tcgaggaaac ccgaagtccc tgccaaggag ttaggccatg
     5101 cagcgttttg tgagatcgat gttcccatcc gccctcggag ccctgctcct ggccgtggcg
     5161 ctgcacgcgc tcgccccagc ggccagggca caaagcggtg agggttggac gacgctcctc
     5221 gacgacaaga ccatgggcga gtggaacagg gtcggggaga gcaattggcg ccacgaagac
     5281 ggtgctgtga ttgccgacaa gaagtccggc caggaccccg gctacctcgt gagcaaaagc
     5341 ccctacaagg attttcagct gcacgtcgaa ttctgggcga gcgatgatgc caacagcggc
     5401 gtgttcctgc gctgccagga tcccagcaag cttggggaca ggtcctgcta cgaggtcaat
     5461 atttttgatc agcgcaagga tcccacctac ggcaccggcg gcattgtcaa tttttccgag
     5521 gtgaacccca tgcccaaggc cggcggcaag tggaacactt ttgagatcac cgccaagggt
     5581 cggctgatca ccgtcgtcct aaatggtcaa aagacggtcg aactgcacaa tgggatgttt
     5641 gtcgaaggcc cgttgggcct gcagcacggt caag
//
LOCUS       DASTMT010000594         4143 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_4807186, whole genome shotgun sequence.
ACCESSION   DASTMT010000594 DASTMT010000000
VERSION     DASTMT010000594.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4143)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4143)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4143
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4807186"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..576)
                     /locus_tag="VFR00_13560"
     CDS             complement(<1..576)
                     /locus_tag="VFR00_13560"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone/menaquinone biosynthesis
                     methyltransferase"
                     /protein_id="HEU0160343.1"
                     /translation="MRKPRRDPAAGEDSEIAAFGFRKVAAETRQGLVNDVFSTVAGRY
                     DLMNDLMSGGLHRLWKDDLITWLNPPRSGRTFSLIDVAAGTGDIALKALRDGGPGCRA
                     VLCDINPAMLEVGRSRLQQAGLAGRAAFAIGNAEALPFADQHFDAFTIAFGIRNVTHI
                     DKALSEARRVLKTGGRFLCLEFSCCDVPMLDR"
     gene            686..1624
                     /locus_tag="VFR00_13565"
     CDS             686..1624
                     /locus_tag="VFR00_13565"
                     /inference="COORDINATES: protein motif:HMM:NF019032.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1499 domain-containing protein"
                     /protein_id="HEU0160344.1"
                     /translation="MIEHGQKIQSRLARWSGRLALFFAGMTLAGVLLHRLSSFPTPVA
                     INVFALSAVGLVLAALLGLLSLIGIWRKGYAGASTAFLGIVLPLLFLGWPLTFLPALF
                     KLPPINDITTDWTSPPRFEALANQRAAVAKPTAYPGEPVAAMQQAGYPDLRPIALDRS
                     TEETFELVEEAVRKLKWRVAASEAPTPKVGRPGTIEATDQTLLLGFPDDIVIRVSGTN
                     QRSRIDVRSASRYGQFDFGQNAARIRRFYGEVQAQVDATLPTTVAGRRRLRTTRGAAM
                     MKKLKERESSKAAPRTPRDRAPSSAQRERVPKETQR"
     gene            complement(1509..2423)
                     /locus_tag="VFR00_13570"
     CDS             complement(1509..2423)
                     /locus_tag="VFR00_13570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU0160345.1"
                     /translation="MTEVLPFKTTMTFAYGEPRQLAAGVVRIVANNPNHFTFKGTNTY
                     LIGTERPALIDPGPDDPAHLAAILSVLGGRRLSHILITHTHHDHVDGLPALIAATGAK
                     TAGYGHKAVRHGAKRTSAAGGEYVEQDFVPDVALRHGDRLEGEGWSFQALFTPGHAPD
                     HLCFALEGTDILFSGDHVMGWNTSVVAPPEGHMGAYIRSLELLTARPERTYFPGHGGQ
                     IEEPQRLVKAYLLHRRMREHAILDCIRQGHDTAEAIVPVVYRGLDPKLLNAASLSVLA
                     HVEHLKERGLVAYEAPLSKTRVLSASSS"
     gene            complement(2513..3091)
                     /locus_tag="VFR00_13575"
     CDS             complement(2513..3091)
                     /locus_tag="VFR00_13575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0160346.1"
                     /translation="MSTADPNKVILTGENPFIRLSPVDSDDYTSNASFWRIIFSPAGP
                     GHVLYVKSELTERRWRIYADNIAMARWLQTTVQGMLNAELKDTAIPVTDAEFSKSGDP
                     RYFWTEKIVAGGEELSLTWFDLGDPLLIHTEPKAVPNRPYGVCTVLVPALGTRLSRNG
                     VEAQGRSWKREREGRPFSTSALAFSESWTDVR"
     gene            complement(3178..>4143)
                     /locus_tag="VFR00_13580"
     CDS             complement(3178..>4143)
                     /locus_tag="VFR00_13580"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013894761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEU0160347.1"
                     /translation="NGAEVTLRPKQLVLATGMSGFPEVPNLPGRDSFQGAQHHSSRYS
                     SGAAYAGKRCVVIGSNNSAHDIAADLWEHGADVTMVQRSPTVVVRSETLIETAHAPLY
                     SEAALKKGISTDIADLILASVPHRLVPERAKALYDDIRERDQDLYSRLEKAGFAFHFG
                     VDGTGIHCIYARRGSGYYIDVGASELVADGRIKLKSGLEVERITPTGVHLSDGSELPA
                     DLIVYATGFGSMNEWAAHLIGAEVADTVGKCWGLGSDTPKDPGPWEGELRNMWRPTQQ
                     EALWFHGGNLQQSRFYSLQLALQIKARAVGIPTPVYGLQRVYHRR"
BASE COUNT          720 a         1326 c         1387 g          710 t
ORIGIN      
        1 ccggtccagc atggggacgt cgcagcagga gaactccagg cacaaaaagc gccccccggt
       61 cttgagcacg cggcgcgcct cggacagcgc cttgtcgatg tgggtgacgt tgcgaatgcc
      121 gaaggcaatg gtaaacgcgt caaaatgctg atcggcaaac ggcagcgcct cggcattgcc
      181 aatcgcgaaa gcggcacggc cggccaaacc agcctgctgc aggcggctgc gccccacctc
      241 aagcatggcc ggattgatgt cgcacagcac cgcccggcag cccggtccgc cgtcgcgcaa
      301 ggccttgagc gcaatgtcgc cggtcccggc ggccacgtcg atcagggaaa aagtgcgccc
      361 ggaacggggc gggttcagcc aggtaatgag gtcatccttc cagagccgat gcaggccacc
      421 ggacatcaag tcgttcatga ggtcgtagcg gcccgcgacc gtggaaaaga cgtcgttgac
      481 aaggccttgc cgcgtttcgg ctgcaacctt gcggaagcca aaggcggcga tctcgctatc
      541 ctcgccggca gcagggtctc tacggggctt gcgcatggac ttgaggtcaa accctagctg
      601 aggttcgacc ccctggccag gctttcgagc gctggccctc ttgccccaac attgctatga
      661 gccgaggcga cgagatgacg gcgctatgat cgagcacggg caaaaaatcc aatcacggct
      721 ggcgcgctgg tcggggcggc tggcgctgtt cttcgccggt atgacgctgg cgggcgtcct
      781 gttgcatcgc ctgagctcgt ttccgacgcc ggtcgccatc aacgtcttcg cgctcagcgc
      841 cgtcgggctg gtgctcgccg ccctgctcgg cctgctgtcc ctgatcggca tttggcgcaa
      901 gggctatgcg ggcgccagca cggcctttct cggcattgtc ctgccgctcc tgtttctggg
      961 ctggccgctc accttcctgc cggcgctttt caagctgccg cccatcaacg acattacgac
     1021 cgactggacc tcgccgccgc gcttcgaagc gctggcgaac cagcgcgccg ccgtcgccaa
     1081 gcccaccgcc taccccggcg agcccgttgc cgccatgcag caggccggct atcccgactt
     1141 gcgccccatc gcgctcgacc gctcgacgga ggagacgttc gagctcgtcg aggaagccgt
     1201 gcgcaagctg aagtggagag tggccgcctc cgaggcgccg acgccgaagg tcggtcgccc
     1261 cggcaccatc gaggcgaccg atcaaacgct gctcttgggt ttccccgacg atatcgtcat
     1321 ccgcgtgagc ggcaccaatc aacgctcgcg catcgatgtg cgctcagcct cgcgctacgg
     1381 ccaattcgat tttggccaga acgccgcccg catccgccgc ttctacgggg aagtgcaggc
     1441 gcaggtcgac gcaaccctgc cgacgacggt ggcgggacgc cgacgcctgc gcaccacgcg
     1501 tggcgcggct atgatgaaga agctgaaaga acgcgagtct tcgaaagcgg cgcctcgtac
     1561 gccacgagac cgcgctcctt caagtgctca acgtgagcga gtaccgaaag agacgcagcg
     1621 ttgagaagct tggggtcgag accgcggtag acgacgggca cgatcgcctc cgccgtgtcg
     1681 tgaccctggc gaatgcagtc gagaatggcg tgctcgcgca tgcgccgatg caggaggtag
     1741 gccttgacga gacgttgcgg ttcttcgatc tggccgccgt gcccgggaaa ataggtgcgt
     1801 tcgggccgtg cggtgaggag ttcgagggag cggatatagg cccccatatg gccttcgggc
     1861 ggcgccacca cgctggtgtt ccagcccatc acgtgatcgc cggaaaacaa gatgtccgtt
     1921 ccctcgagcg caaagcagag gtgatccggc gcgtgccccg gcgtgaacag cgcctggaat
     1981 gaccagccct cgccttcgag acggtcgccg tgtctcaagg ccacatccgg cacaaaatcc
     2041 tgttccacgt actcgccccc agcggcactg gtacgcttgg ctccgtggcg caccgcctta
     2101 tggccgtagc ccgcggtttt cgctcctgtt gccgcgatca gcgccggcaa tccatcgacg
     2161 tgatcgtgat gggtgtgcgt aatgagaata tgggaaaggc gccgtccccc aagcacgctc
     2221 aggatcgcgg cgagatgcgc ggggtcatcg gggccggggt cgatcagagc cgggcgctcc
     2281 gtgccgatca ggtaggtatt ggtgcccttg aaggtgaagt ggttggggtt attggcgaca
     2341 atgcgcacga cccccgcggc gagctggcgc ggctcgccat aggcaaaggt catcgtcgtc
     2401 ttgaagggca ggacttcggt catcagcggt tcccaggtga gccaaaggcg ggcgaccgag
     2461 gggctacagg ccggagcccc ccgttgcaag caataatccg ccgccgcccg ggctagcgga
     2521 cgtcggtcca gctttccgag aaggcaagcg ccgaggtgct gaacggccgc ccctcacgct
     2581 cgcgcttcca agagcggcct tgcgcttcga caccgttgcg ggagaggcgg gtgccgagcg
     2641 ccggcaccag caccgtgcac acgccataag gccggttcgg caccgccttg ggttcagtat
     2701 ggatcagcag cggatcgccg agatcgaacc aggtgaggct caactcttcg ccgccggcga
     2761 cgattttctc ggtccagaag tagcgtggat cgccggattt gctgaactcg gcatcggtca
     2821 cgggaatggc cgtgtccttg agctcggcat tgagcatgcc ctgcacggtc gtttgcagcc
     2881 aacgcgccat ggcaatgttg tcggcataga tccgccagcg ccgttcggtg agctcgctct
     2941 tgacgtagag cacgtggccg ggccctgcgg gcgaaaagat gatgcgccag aagctggcgt
     3001 tgctggtgta atcgtcgctg tcgacggggc tcaaccggat gaaagggttc tcgccggtca
     3061 ggatcacttt gttggggtcg gcggtgctca tggggtctcc tccctgggct ttatggcagc
     3121 ggggagcatg gcaccggcgc cgagcccggg caacggccaa ggccgcaagc gccgggctca
     3181 gcggcgatga tagacgcgct gcaagccgta gacgggcgtg gggatgccga cggcgcgtgc
     3241 cttgatctgc agcgcgagtt gcaaggaata gaagcgggat tgctgcaggt tgccaccatg
     3301 aaaccagagc gcctcttgct gggtgggccg ccacatgttg cgaagttcgc cctcccaagg
     3361 cccggggtcc ttgggggtgt cggagccgag cccccagcac ttgccgacgg tgtcggcgac
     3421 ctcggcgccg atcaggtgcg cggcccactc gttcatggag ccaaagcccg tggcgtagac
     3481 gatcagatcg gcggggagct cgctgccgtc gctcaaatgc acgccggtcg gcgtgatgcg
     3541 ctccacttcg aggccgcttt tgagcttgat gcggccgtcg gcgacgagtt cgctggcacc
     3601 aacgtcgatg tagtagcccg agcctcgccg cgcatagatg cagtggatgc ccgtgccgtc
     3661 gacgccgaag tggaaagcaa aacccgcctt ctccagccgg ctgtaaaggt cctggtcgcg
     3721 ctcgcggatg tcgtcataaa gcgcctttgc ccgttccggc acgagacggt gcggcacgga
     3781 ggcaaggatc aggtcggcga tatcggtgga gatgcctttc ttgagcgcag cctccgaata
     3841 aagtggcgcg tgcgccgtct cgatcagggt ttccgagcgc acgaccacgg ttggggaacg
     3901 ctgcaccatc gtcacgtcgg cgccgtgctc ccagagatcg gcggcaatgt cgtgcgcgga
     3961 attgttcgaa ccgatcacca cgcagcgttt gcccgcgtag gccgcccccg agctgtagcg
     4021 gctggaatgg tgctgcgcgc cctgaaagct gtcccgaccg gggaggttcg gcacctcggg
     4081 gaagcccgac atgcccgtcg ccaggaccag ctgcttgggt cggagcgtga cttcagcacc
     4141 gtt
//
LOCUS       DASTMT010000595         3229 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_6125000, whole genome shotgun sequence.
ACCESSION   DASTMT010000595 DASTMT010000000
VERSION     DASTMT010000595.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3229
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6125000"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(223..1500)
                     /gene="eno"
                     /locus_tag="VFR00_13585"
     CDS             complement(223..1500)
                     /gene="eno"
                     /locus_tag="VFR00_13585"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010969226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEU0160348.1"
                     /translation="MTAIVDIIGREILDSRGNPTVEVDVVLDGGARGRAAVPSGASTG
                     AHEAIELRDRDPKRYAGKGVLKAVEAVNGEIFEALGGMDAEDQRRIDACLKTLDGTPD
                     KSRLGANAILGVSLAVAKAAAEAHALSFYRYLGGANAHLLPVPMMNIINGGAHADNPI
                     DIQEFMIMPVGAASCAEAIRMGAEVFHTLRSALHAAGHNTNVGDEGGFAPNLKSAEEA
                     LAFITKAIETAGYKPGDDMVLALDCAASEFCKDGKYVLAGEGKSLDSGGMVNYLAGLV
                     ARFPIVSIEDGMAEDDWQGWQALTEKIGARCQLVGDDLFVTNTARLAEGIGKGIANSI
                     LIKVNQIGTLTETLEAVALAQSSHYTAVISHRSGETEDASIADITVATNAGQIKTGSL
                     SRSDRLAKYNQLIRIEQDLGDQARYAGRGVLRR"
     gene            1662..2369
                     /gene="queC"
                     /locus_tag="VFR00_13590"
     CDS             1662..2369
                     /gene="queC"
                     /locus_tag="VFR00_13590"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011503156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HEU0160349.1"
                     /translation="MAQAAALVLFSGGQDSTVCLAWALERYARVETVGFAYGQRHAIE
                     LQQRPIIRAELAARFESWRARLGEDHVLELMTLGEISDTALTRDAAFAMTAAGLPNTF
                     VPGRNLLFCTLAAALGFRRDITVLIGGMCETDYSGYPDCRNDTLQALAKAISLGLDRP
                     VRIETPLMWIDKAATWSLAARLGGSALVELIIEHTHTCYRVERGRRHPWGYGCGDCPA
                     CSLRAKGWEKWQLTARS"
     gene            2345..2596
                     /locus_tag="VFR00_13595"
     CDS             2345..2596
                     /locus_tag="VFR00_13595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0160350.1"
                     /translation="MATDRQELICAVELAIAGDWQGAHKIVQQDETDQTSCWLHAVLH
                     KIEGDAANSRYWYGRAGQAYEAYADAKAELTAIKAALTY"
     gene            2685..>3229
                     /locus_tag="VFR00_13600"
     CDS             2685..>3229
                     /locus_tag="VFR00_13600"
                     /inference="COORDINATES: protein motif:HMM:NF039315.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5996 family protein"
                     /protein_id="HEU0160351.1"
                     /translation="MDQQPAWPELPFAAWSETCETLHRWTQVAGKIALALTPLVNHWW
                     NVTLLPTARGLSTPPLPWRASTFTIDFDFLAHRVDVISSRGTTAGLPLQAMPVAQFYA
                     ELMACLQRLGITVKIWTMPSEIVDAVPFERDLTNAAYDPIWVERFWQALSQSDRVLKQ
                     FRARFIGKVSPVHFFWGSFDLA"
BASE COUNT          548 a         1018 c         1086 g          577 t
ORIGIN      
        1 aggcagacaa gcgacaccag gatctgccgg ggttggcttt tgcgccgatc atgcatgaag
       61 gtgacgcgaa accgttgggg tggtgcgccc ctggcggggc gcggacaaca gtttcaccca
      121 aggctggtta aacgcgggta aagcgcgcgg ggcatcagat gcaaaagaag aggccggcgc
      181 gccagcgcgc ccgccggcca agcgcggcaa ggcgctgcgg cgctagcggc gcagcacgcc
      241 gcgcccggca tagcgggcct ggtcgcccaa gtcctgctcg atgcgaatga gctgattgta
      301 tttggcgagc cggtccgatc gcgacagcga gccggttttg atctgcccgg cgttggtggc
      361 caccgtgata tcggcgatgc tggcgtcctc ggtctcaccg gagcggtgcg aaatgacggc
      421 cgtgtagtgg gacgattgcg ccaaggcgac ggcttccagc gtctccgtca gggtcccgat
      481 ctgattgacc ttgatgagga tcgaattggc gatgcctttg ccaatgccct cggccagccg
      541 ggcggtgttg gtgacgaaga gatcgtcgcc gacgagctgg cagcgcgccc cgatcttctc
      601 ggtcagggcc tgccaaccct gccagtcgtc ctccgccatg ccatcctcga tcgacacgat
      661 ggggaagcgg gcgacgaggc cggcgaggta atttaccatg ccgccgctat ccaagctttt
      721 gccttcgccg gccaagacat acttgccgtc cttgcagaat tcgctggcgg cgcaatcgag
      781 cgccaagacc atgtcgtccc ccggcttgta gccggcggtc tcgatcgcct tggtgatgaa
      841 ggccagcgct tcctcggcgg atttcaggtt gggcgcaaag cctccttcgt cgccgacgtt
      901 ggtgttgtgg ccggcggcat gaagcgcgct acgcagggta tgaaacacct ccgcccccat
      961 gcggatcgcc tccgcacagc tggccgcgcc cacgggcatg atcatgaatt cctgaatgtc
     1021 gatgggatta tccgcgtgcg cgccgccgtt gatgatgttc atcatcggca ccggtaacag
     1081 gtgggcattg gccccgccga gatagcgata aaacgaaagt gcgtgcgcct cggccgcggc
     1141 cttggccact gccagcgaca ccccaaggat ggcattggcg ccgagccggc tcttgtccgg
     1201 ggtgccgtca agggttttga ggcaggcgtc gatgcggcgc tggtcctccg cgtccatgcc
     1261 gcccagcgcc tcgaagattt cgccattgac ggcctcgacg gccttgagca cgcccttgcc
     1321 cgcgtaccgc ttgggatccc gatcgcgcaa ttcgatggcc tcgtgcgcac ccgtcgaggc
     1381 gccggacggg acggccgcgc gcccgcgcgc gccgccgtcg agaacgacat cgacctcaac
     1441 ggtcggattg ccgcggctgt ccaggatttc gcggccgata atatcgacga tggctgtcat
     1501 cagggggttc ccagctcgag cacgatgttt tgaggcgacg gcaggcctgt gagaagctat
     1561 gccgcgtggt tctagcccta tcggccgcga cccgccaggc ggcagttcgc gcctgtcgaa
     1621 gggtcagttc ccccgtcttg gcacgagagc acaaccggag catggcgcag gcagcggcgt
     1681 tggtgttgtt ttcgggcggg caggattcca ccgtatgcct ggcctgggcg ctcgagcgct
     1741 acgcccgggt ggagaccgtc ggctttgctt atgggcagcg ccacgccatc gaattgcagc
     1801 agcgcccgat catccgtgcc gagctcgcgg cgcgtttcga gagctggcgc gcgcgcctgg
     1861 gcgaagacca tgtcttggag ttgatgacct tgggcgagat cagcgacacg gcgctgaccc
     1921 gggacgcggc ctttgccatg acggcagccg gcctgcccaa caccttcgtg cccgggcgca
     1981 acctgttgtt ttgcacgttg gcggcggcgc tcgggtttcg gcgcgacata accgtcctga
     2041 tcgggggcat gtgcgaaacc gactactcgg gttatcccga ctgtcgcaac gacaccttgc
     2101 aagccttggc caaggcgatt tcgctcggcc tcgacaggcc ggtccggatc gagacgccgc
     2161 tgatgtggat cgacaaggcc gcaacctgga gcctggctgc ccgcttgggc ggatcggccc
     2221 tggtcgagct catcatcgag cacacccaca catgctatag agtagagcgc ggcaggcgcc
     2281 acccctgggg gtacggctgc ggcgactgtc cggcgtgcag cctgcgggcg aaaggatggg
     2341 aaaaatggca actgaccgcc aggagctgat ctgcgccgtc gagctggcca tcgccggcga
     2401 ttggcagggt gcgcacaaga tcgtgcagca agacgagacc gatcagacca gttgctggtt
     2461 gcatgccgtg ctgcacaaga tcgaagggga cgcggccaat tctcgctact ggtacgggcg
     2521 cgccggccag gcctatgagg cttatgccga cgccaaggcc gagctcacgg cgatcaaggc
     2581 ggcgctgaca tactgataaa agcgcaggtt cagggggcgg caggggcgca agcgttcggt
     2641 gggacgcaag ccccggtaaa cggcaacagg agagcccctc gagcatggac cagcaacccg
     2701 catggccgga gcttcctttc gccgcctgga gcgagacgtg cgagacgctg catcgctgga
     2761 cccaggtggc gggtaagatt gcgctcgcgc tcacaccctt ggtcaaccat tggtggaacg
     2821 tcacgctgct gccgacggca cggggcctga gcacgccgcc cctgccctgg cgggccagca
     2881 cgtttacgat cgacttcgat tttttggcgc atcgcgtaga tgtcatcagc agtcgcggca
     2941 ccacggcagg cttgcccctg caggcgatgc cggttgccca gttctacgcc gagctgatgg
     3001 cgtgcctgca gcgccttggc atcaccgtga agatttggac gatgcccagc gagattgtcg
     3061 acgcggtgcc cttcgagcgg gatttgacca acgccgccta cgatccgatc tgggtggagc
     3121 ggttttggca ggcgctgtcg cagtccgatc gggtactcaa gcaatttcgc gcccgcttca
     3181 tcggcaaggt gagccccgtg cacttctttt ggggcagctt cgatctcgc
//
LOCUS       DASTMT010000596         1525 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_5142015, whole genome shotgun sequence.
ACCESSION   DASTMT010000596 DASTMT010000000
VERSION     DASTMT010000596.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 1525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1525
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5142015"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            125..937
                     /locus_tag="VFR00_13605"
     CDS             125..937
                     /locus_tag="VFR00_13605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012497166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEU0160352.1"
                     /translation="MPKAGNVRVGVGGWTFEPWRGVFYPEGLAHAKELAYASSKLTTI
                     EINGTFYRTQSPATFAKWRKDVPDGFVFSVKAPRYVVQKRVLAEAGEALEFFFQSGVS
                     ELGDRLGPILWQFAPTKKFDAGDFAAFLAMLPAKRDGLDLRHVVEVRHPSFATPQFIS
                     LLRRHKTPVVFAEHDTYPAIADVSGDFIYARLQKGSDALATGYPPKELDAWAKRAQTW
                     AAGGAPTDLPPIDAKPAKKAARDVFLYVIHEGKIRAPAAAMALIERLGGRHG"
     gene            complement(975..>1525)
                     /locus_tag="VFR00_13610"
     CDS             complement(975..>1525)
                     /locus_tag="VFR00_13610"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HEU0160353.1"
                     /translation="TRALDNGALGIVVPHVDTPAQARSLVEAFRFSPIGRRSTGGSNA
                     CFGYRAPPAAEAQKTLNAEILVVAMIETPEAVENAKAIAAIDGIDVLLIGSNDLSLEM
                     GIPGQVGHEHVRAAYEQVAAACRQHGKVLGMGGVYDQEVASRYIALGARMILGASDHS
                     LLLEAATRRAAFLRGLLPETGA"
BASE COUNT          258 a          526 c          494 g          247 t
ORIGIN      
        1 tggtgatgcg tgcgcgcgat cggcgctggc acggtccgaa tgtagccgcc gcgcccccct
       61 tgcgctagca accacttttg ccgagcatgg cacgacagcg cgtcgccgca atgggggcta
      121 gagcatgccg aaggcaggca acgtcagggt cggcgtcggt ggctggacct ttgaaccctg
      181 gcgtggggtg ttctatcccg agggtctggc gcacgccaag gaactcgcct acgcgagctc
      241 caaactcacc accatcgaaa tcaacggcac gttctaccgc acgcaatccc ccgccacctt
      301 cgccaaatgg cgcaaggacg tgccagatgg cttcgtgttc tccgtcaagg ccccgcgcta
      361 cgtcgtgcag aagcgcgtgc tggccgaggc cggcgaggcg cttgaatttt ttttccagtc
      421 cggcgtgagc gagctgggcg accggctggg gccgatcctg tggcaattcg ctccgaccaa
      481 aaagttcgat gccggcgact ttgccgcctt tttagccatg ctgccggcca agcgcgacgg
      541 gctcgacttg cgccacgtgg tggaagtgcg ccacccgtca ttcgcaacgc cgcaattcat
      601 ctcgctgttg cgtcggcaca agacgccggt ggtgtttgcc gagcacgaca cctatcccgc
      661 catcgccgac gtgtccggtg actttattta cgcccgccta caaaagggca gcgatgccct
      721 cgctaccggc tacccgccca aagagctcga cgcctgggcc aagcgcgcgc agacctgggc
      781 cgcgggtggc gcgccgaccg atctgccgcc gatcgatgcc aagccggcca agaaggccgc
      841 gcgcgacgtc ttcctctacg ttatccacga aggcaagatc agagcccctg ccgccgccat
      901 ggcgctgatc gagcggctgg gcggccgcca cggctaaccg cgcaggcgag agcggggggc
      961 ggacccgcca gggctcaggc gcccgtttcc ggcagcaggc cgcgcaagaa cgcggcgcga
     1021 cgcgtcgctg cctccaacaa taagctatgg tcgctggcgc cgaggatcat gcgcgcgccg
     1081 agcgcgatat agcggctcgc cacctcctga tcataaacgc cccccatgcc cagtaccttg
     1141 ccgtgctgac ggcaggcggc cgcgacctgt tcgtaggccg cacgcacatg ctcgtggccg
     1201 acctgacccg ggatccccat ctcaagcgag aggtcgttgc tgccgatcaa cagcacgtcg
     1261 atgccgtcaa tggcggcgat cgccttggca ttctcgaccg cctcgggcgt ttcgatcatc
     1321 gccaccacga ggatctccgc attcagcgtc ttctgagcct cggcggccgg cggcgcgcga
     1381 tagccgaagc aggcgttgga tccaccggtg ctgcggcggc cgatcggact gaagcggaag
     1441 gcctcgacca ggctgcgcgc ctgggcgggc gtatcgacgt gcggcacgac gatgccgaga
     1501 gcgccattgt cgagggcgcg ggtgc
//
LOCUS       DASTMT010000597         5130 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_3819174, whole genome shotgun sequence.
ACCESSION   DASTMT010000597 DASTMT010000000
VERSION     DASTMT010000597.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 5130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5130
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3819174"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(15..725)
                     /locus_tag="VFR00_13615"
     CDS             complement(15..725)
                     /locus_tag="VFR00_13615"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031966 - mitochondrial membrane
                     [Evidence IEA];
                     GO_process: GO:0033617 - mitochondrial cytochrome c
                     oxidase assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEU0160354.1"
                     /translation="MPLKAKCSRAVPWPRRQTKRCEPHGRRSSPAPRALPNLVAIVLC
                     AGVALLPAAGAAGAQQPRPAPLQALSFVDADGHPVLSTDFPGKWLLVYFGYMHCSDLC
                     PTGLSVLASALDQLGPAADAVQPLFVTVDPERDRGPELRRFAEAFDKRLRGLSGTLAE
                     IKRAATALGVAFEKVAQPGTDYVIDHSSLYVLIDPTLRHVRSLRVAEPHLLAAKLIEH
                     MSAAGTPLGPLNNLGAYR"
     gene            complement(841..1281)
                     /locus_tag="VFR00_13620"
     CDS             complement(841..1281)
                     /locus_tag="VFR00_13620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0160355.1"
                     /translation="MLGHGLTPDTTKTGRGWATLAVARLWRRRQRYPRGEAARTDPAF
                     APEQVLPAIVMRARDDARSRRALAQLGYVKVRAHYTRCKREGETTFRALEHQRLAPSM
                     DLVRDWLAEERKRIVLATRWPFLMTMLVTIVAGIVFAAVARVLE"
     gene            1900..2658
                     /locus_tag="VFR00_13625"
     CDS             1900..2658
                     /locus_tag="VFR00_13625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0160356.1"
                     /translation="MLPRAREVLLSDWRWLIGEVGLGVLVVLIALHATGLVDVARFIQ
                     DYQLLIGTLVGIGATGAVGFWTITTTLRSNAENALAQREREIELERVGARAMIMAELM
                     VMHEGLSTMHQVCMEGRKRNNFTVPKFLGGAELYQGYRGQIAKVGLLPKDQLLSVVWA
                     YATQEGMYANWKWMEQLQEAYDTSKLNQPHEVFITGLNSLLRRVETAIVALGGEVMPR
                     NRVGLPEPTSTAALPGPDDRPLAAIERRVIAMAQ"
     gene            complement(2688..3662)
                     /locus_tag="VFR00_13630"
     CDS             complement(2688..3662)
                     /locus_tag="VFR00_13630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014493052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEU0160357.1"
                     /translation="MLSRRRFASALAALAGSRLSGEPARAQSYPARAITLIVPFAAGG
                     FNDVIVRIVAQHMSKTLGASIIVENDAGAGGTTATARGAAAAADGYTLIAGSMGTHAA
                     APTQYPNLKYDPAKDFTPIGLTAEAPAVLVTRKDFPANTLAEFVAYVKSNQAKVNEAH
                     AGVGSQMHTYCTLLHTLMGTKTARIAYRGGAPAINDLVAGQVDFGCVSVNTVVSQIQG
                     GTVKALAIASPQRAEVIKDVPTAAEAGLPEFQVSGWNAIFAPRHLPAAIHAKLSEALN
                     GALEDAATQKRLLEIGCIVPERADRSPDALQQRVEREVARWSSILKRE"
     gene            complement(4583..>5130)
                     /locus_tag="VFR00_13635"
     CDS             complement(4583..>5130)
                     /locus_tag="VFR00_13635"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEU0160358.1"
                     /translation="RYNFALPKDLGDMRFGAVGTNIAADEGGAKVLKAIEGAPAARAG
                     VLAGDVITHVDGASLAGLSLNQMIVKLRGPVGSSARLQVQRKNQAAPLDIVVGREVNR
                     AVLKVRAEQGKLVIEAVGGRQVFEFEMGAPVAVVPLSDSAFYVDGRYHTQIVFTRDSS
                     GGVSGVILNPGPWEQKGVRED"
BASE COUNT          883 a         1621 c         1718 g          908 t
ORIGIN      
        1 aatttcgccg ccgctcaacg ataggcgccg agattgttga ggggaccgag cggggtgccg
       61 gcggcactca tgtgctcgat cagcttggca gcgagcaggt gcggctcggc cacccgcaag
      121 gagcgcacgt ggcgcaacgt cggatcgatc agcacgtaga gcgagctatg gtcgatgacg
      181 taatcggtgc cgggctgcgc caccttctcg aaggcgacac cgagcgcagt ggccgcccgt
      241 ttgatctcgg ccagcgtgcc tgagagcccg cggaggcgtt tgtcgaaggc ttcggcaaag
      301 cggcgaagct cgggcccgcg atcgcgttcg gggtcgacgg tcacgaacaa cggctgcacg
      361 gcgtccgcgg cgggcccgag ctggtcgagc gcgctagcaa gcacgctcaa gccggtcggg
      421 cacaggtcgg agcagtgcat atagccgaaa tagacgagca gccatttgcc gggaaaatcg
      481 gtgctgagaa cggggtgccc gtcggcgtcg acgaagctca aggcttgcaa aggggcgggc
      541 cgcggttgct gggcgcccgc cgcgccggca gcgggcaaga gcgcgacacc cgcgcagagc
      601 acgatggcca caaggttggg caaggcacgc ggggcggggc tgctgcggcg gccgtgcggc
      661 tcgcagcgct tggtttgccg ccggggccat ggcaccgccc gtgaacactt ggctttgagg
      721 ggcacgtcct gacctccacg ctgggccacg gccgcagcgc acggagaagc tgcccgctgc
      781 ctccggcacg cgcaaggtgg ggcttatgac aaaagcgtgt ttgtcggcac ttgcagggct
      841 ctattcgagc acacgcgcga cggcggcaaa gacgatgccg gcgacgatcg tgaccagcat
      901 ggtcatcagg aacggccaac gcgtcgccaa gacgatacgc tttcgttcct cggccagcca
      961 gtcgcgcacg aggtccatac tgggggccag gcgctggtgc tcgagggcgc gaaaggtggt
     1021 ctctccctcc cgtttgcagc gcgtgtagtg ggcgcgcacc ttgacatagc cgagctgagc
     1081 tagcgcccgc cgggagcgcg catcgtcgcg cgctcgcatg acgatggcgg gcaaaacctg
     1141 ttccggggcg aaggccggat cggtccgggc cgcctcgccg cgggggtacc gctgtcgccg
     1201 gcgccacagg cgcgcgacgg caagggtagc ccacccccgc cccgttttgg tggtgtcggg
     1261 tgtaagccca tgacccagca tgcaacgaga ccccctgacg caagagcatg gccgcaacgc
     1321 cgcgcgcaag aaaagtgggc aaatgcggac gactgaaggc ggagcttggc gtttgtcgcc
     1381 gcagtggcgc taggatcaca gccagggacc tgggagccgc cgccgtctcg gcccgcctcg
     1441 gcagcgctgt gcgatggccg cccgaggaaa ctgcgcgcga gggcccaggc aatgttacgc
     1501 gatcgacatg ccacggcgag cgcacctggg ccggggcctg tcgacctgag gcggtgcggt
     1561 ggagccacaa cgccggcgtt gcccgggtga gccaccagcc tttcccaggc aaagagtgta
     1621 tatttttcgc aatcgtcgcc gaccgcgcta caaatttttc aggaccgggc gcaatgacgc
     1681 gcgctctatt ctttcaccgc ggcgtgcgcg gtggcgctgg cgttctcggc gcacgggcgc
     1741 cctcgtgtcc ccaacgccgc agcgccgccg agattgtgac tgaagtcggg cttggtcacc
     1801 ggtctgccgc aatggcccgt gaggctgggg ggatggcgca tcccttggcg cggggtgggt
     1861 gcgagaacag tttgttttca tcgattgatg aggactgtga tgctcccgag agcgcgtgag
     1921 gtactccttt cggattggcg ttggctgatc ggggaggtgg gcttaggggt tttggtcgtg
     1981 ctgatcgcct tgcatgccac cggccttgtc gatgtggcgc gcttcatcca ggactatcag
     2041 ctgctgatcg gcacactggt cggcatcggc gccacgggtg ccgtgggatt ttggaccata
     2101 acgacaaccc tgaggagcaa cgcggaaaac gcgcttgccc agcgcgagcg cgagatcgag
     2161 ctcgaacgcg tgggcgcgcg tgccatgatc atggccgagc tgatggtcat gcatgagggg
     2221 ctctcgacca tgcaccaggt atgcatggag gggcgcaaac gcaacaattt cacggtgccc
     2281 aaattcttag gcggcgcgga actctatcag ggctatcgcg gacaaatcgc caaggtcggc
     2341 ctgttgccca aggatcagtt gctgtcggtc gtgtgggcct atgccacgca ggaaggcatg
     2401 tatgcgaact ggaaatggat ggagcagctg caggaagcct acgacacgag caagctcaat
     2461 cagccgcacg aagtcttcat cacgggtttg aacagcctat tgcgccgcgt cgagacggcc
     2521 atcgttgcgc tgggtggtga agtaatgccg cgcaaccggg tgggtttgcc ggaacccacg
     2581 agcaccgcgg cgttgcctgg acccgacgat cgtcccctgg ccgcgatcga gcggcgcgtc
     2641 atcgccatgg cccaatgagg gcaggccgct cgagttttcg cgcggcctca ttcgcgtttt
     2701 aagatggacg accaacgggc gacctcgcgc tcgacccgct gttgcagggc gtccgggctg
     2761 cgatcggcgc gctcggggac gatgcagccg atctccaaca gccgcttttg cgttgccgca
     2821 tcctccaggg cgccgttcag cgcctcgctc aatttggcgt ggatcgccgc gggcagatgg
     2881 cgcggcgcga agatggcatt ccagcccgac acctggaatt cgggcaatcc cgcctccgcc
     2941 gcggtgggca cgtccttgat gacctcggcg cgctgcgggc tggcgatggc gagcgctttg
     3001 accgtgccgc cttggatttg cgacacgacg gtgttgacgg acacgcagcc gaagtcgacc
     3061 tggccggcca ccagatcgtt gatggcaggc gcaccgccgc gataggcgat gcgagcggtc
     3121 ttggtcccca tcagggtgtg cagcagcgtg cagtaggtat gcatctgcga gccgacgccg
     3181 gcgtgcgctt cattgacctt ggcctgattg cttttcacgt aagcgacgaa ctcggccagc
     3241 gtattcgccg gaaaatcctt acgcgtgacg agcaccgccg gcgcttccgc cgtcaggccg
     3301 atcggcgtga agtccttggc cggatcgtac ttcaggttgg ggtactgcgt cggggccgcc
     3361 gcgtgcgttc ccatgctgcc ggcgatcaag gtatagccgt ccgcggccgc ggcagcgccg
     3421 cgcgccgtgg cggtggtgcc gccggcgccg gcgtcgttct cgacaataat cgaagccccg
     3481 agtgtcttgc tcatatgctg ggcaacgatg cgcacgatca cgtcgttgaa gccgccggcg
     3541 gcaaagggaa cgataagggt gatcgcgcgc gccgggtagg attgggcgcg cgcgggctcg
     3601 ccgctgagtc tgctgccggc gagggcggcc agtgcggaag cgaaacggcg tcggctgagc
     3661 atgggctgcg gcctccctcg gctagcgggc cccgccagag tatggccaac cccgggcaag
     3721 atccattccc ctcctcggga ggtgtgtcct cgcgggcgcg gcagatgacg gcggcgggcg
     3781 ggcttcgcga ttgaccgcgc atgtgggtgt gcgcggggag aggtagaggt ccacttggaa
     3841 tgagatgaca ggagccctca ccgatgagtc agacacccat cagcgaactc gaacgctgat
     3901 ttgccgttcc acccgcagca cttatcgcgt ttttgccgct gcatcgtcgg ccgcgttatc
     3961 gctcgcccgt ttcttccccg tatggcgcag ttcgccgggc cattcgggtc gtcgcaaatt
     4021 acgctcgtgc tcgccaccaa gctgatcaac tctctcgaca agtctgatcg tcccacgccc
     4081 cggtcgccgc gctccaccga caccggcggc gcacccgcaa attcccatag cactccggcc
     4141 cgaccggcaa caatctctcg cggccggcct ccatgtgtgc tgcgccgtct tcatgggtcg
     4201 gcgtctgaaa accttcacac taacggacat gcatcgcgct ctgctggcgc ccgacgctat
     4261 ctcaggaatc tttgcccacg aggctgcact ttgtccccgg agacaagccg gctgttcctg
     4321 ggaaatcgcg cggcgtgaag gtgtcgttgg caccccatcg agagcttgga gacagtgtcg
     4381 ctacggctta gcttgcgctc tgcccaaacc agagggaata gtaaaacata ttgcggggcg
     4441 gaggggcgtg tttccgtgca cgcaactcag cgaaccttgg acagcttctg gtcatgggat
     4501 gctttccgac gccggcccac aatgtgtgcc gcgccgtctc catgggccgg catctgaaag
     4561 cctttacacg agcagacctg catcaatctt ctctcactcc tttctgttcc catgggccgg
     4621 ggttcagaat gacgccggac acccctccag aactgtccct cgtgaaaacg atctgcgtat
     4681 ggtatcggcc gtcgacgtag aacgctgaat ccgacaatgg gacgacggcg accggcgcgc
     4741 ccatttcgaa ttcaaacact tgccgtccgc cgaccgcctc gatgacgagc ttgccttgct
     4801 cagctcgaac cttaaggacg gctctgttga cctcgcgccc aactacgatg tcgagcggtg
     4861 cagcctggtt cttgcgctgg acctgcagcc gagcgctgct acccacgggg ccgcgtagtt
     4921 taacgatcat ctgattgagc gagagacctg caagagaagc accgtccaca tgcgtgatca
     4981 cgtcgcccgc gagaaccccc gctcttgctg ccggggctcc ctcgattgct ttcaggacct
     5041 tggctccgcc ctcgtcggcg gcaatgttcg tcccgacggc gccgaaacgc atgtccccta
     5101 gatccttggg cagagcaaaa ttgtatctac
//
LOCUS       DASTMT010000598         8028 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_5143208, whole genome shotgun sequence.
ACCESSION   DASTMT010000598 DASTMT010000000
VERSION     DASTMT010000598.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 8028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8028
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5143208"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            669..743
                     /locus_tag="VFR00_13640"
     tRNA            669..743
                     /locus_tag="VFR00_13640"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:701..703,aa:Val,seq:gac)
     gene            771..896
                     /gene="ykgO"
                     /locus_tag="VFR00_13645"
     CDS             771..896
                     /gene="ykgO"
                     /locus_tag="VFR00_13645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012177066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HEU0160359.1"
                     /translation="MKVRNSLKSLRGRHRANRLVRRKGRVYIINKTNRRFKARQG"
     gene            951..1643
                     /locus_tag="VFR00_13650"
     CDS             951..1643
                     /locus_tag="VFR00_13650"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU0160360.1"
                     /translation="MAAMMLRLTSFVAAVAVGASLLGGTPGSWAQTQPTPPQTPNPKE
                     GTPPAITPPAPGAKRGSAALPKTPEEREKTLSDLYALLATADDEDSAKAIAEAIERIW
                     LHSGSATIDLLMERSMKAVSEKNQELALKLLDHIVELAPDFTEAWNRRAYVHFVRNEI
                     ELALGDLRRALALDPNHFKALDGLGQILREIGQKKAALQAFKQLLEVHPFWSGAQQLI
                     DELARDVEGQGI"
     gene            1894..2502
                     /locus_tag="VFR00_13655"
     CDS             1894..2502
                     /locus_tag="VFR00_13655"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU0160361.1"
                     /translation="MLRIDYLSIGGLPPLSFEVAPGQCLVIEGPSGSGKTRLLRAIAD
                     LDPAEGYVFLEGVERSEMPASLWRRRVRFVSAEPAWWAATPRAHFSPAAKLDRLLAAL
                     GLEHLNLDQPVAELSTGERQRLALVRALADDPPVLLLDEPTSALAATNAALTSEFIKF
                     QILAGRIVILVSHDAVQIERLASARLHLADLEWPHAPESAAA"
     gene            2499..3311
                     /locus_tag="VFR00_13660"
     CDS             2499..3311
                     /locus_tag="VFR00_13660"
                     /inference="COORDINATES: protein motif:HMM:NF015605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0160362.1"
                     /translation="MSLPLANTDLLWAALLIALNGAISLTFGLRLELSLALIALRMVV
                     QVAALGVVLRFVFLQSSPLWTAAVALLMLLIAGYELAERQERRFRGWWAYGLGNATLL
                     FASGLATLYAVTVVMAPSPWYAPRYLLPVLGMVLGSTLTSVSLVLQTLAEGIERERGA
                     IEARIAQGGTRFEAFAPLMRRAMRTAITPLLNIMSVAGMVTLPGMMTGQILAGADPVE
                     AARYQIVLMFILAGAAGLGAFATAVGGVLMISDRRHRVRLDRLTSLASWRPF"
     gene            complement(3308..3841)
                     /locus_tag="VFR00_13665"
     CDS             complement(3308..3841)
                     /locus_tag="VFR00_13665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019197244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEU0160363.1"
                     /translation="MGLADITNVVLEFVRAHPNWASAIVFILAFGESLAFVSLVLPFW
                     AMLVGIGAIVAAANPLIFWSIVTSAATGAALGDWVSYWLGNHYHEQIQKMWPLKNHPK
                     LIENGRAFFKRWGVWAIVLGRFSGPLRASVPIVAGTIEMPWLRFQIANWGSAFLWAYV
                     LLAPGSAGMKWWLDRLG"
     gene            4054..5163
                     /locus_tag="VFR00_13670"
     CDS             4054..5163
                     /locus_tag="VFR00_13670"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEU0160364.1"
                     /translation="MGVVAVAGVRHRANRRLALLVLCGANLALVVAIAGSMTVLSDVL
                     APLTGHFMGVGLAASLGLLTRRLCALVLGILATFLVHAWLGLAGCCHPPQPGMVTGGG
                     LSTIAAAGGSHDLSLVALNAWHHHGEPARLSSYLADVDADLVVLSEFGPNKRALLEPL
                     KRSHPFQIDCADDWSCSLALLSRIPFASAGTGHIAEGKLAFVWARLNGGVTVLGTHLH
                     RPSRDPWLHEQQMLELVQFIDRIPGPVILAGDLNTTPWSKSYRTLRRVAGLVPASTLR
                     PTWPAWPLAFPQVALDHIFVSPDLAVSAAGTGPAVGSDHLPIWAVVQRRPSFDRSKAA
                     THRFASGFAPPQPHFRAQLLADFSGEHAGTRNLRR"
     gene            complement(5071..5559)
                     /locus_tag="VFR00_13675"
     CDS             complement(5071..5559)
                     /locus_tag="VFR00_13675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0160365.1"
                     /translation="MGSVREPQEETMPLAIGRLSKQSVLGALSALALISTGCSGLFGP
                     DDGRAGLACLDDSPECVEQRQVALKSMIADKDRKWVREPATPEAHASGVRLFAFRSRK
                     KELSCEELAYGKREADNAPKALRGPEGKALSPAQISRASMFATEVSKELGAEMRLRRC
                     KA"
     gene            5693..6664
                     /locus_tag="VFR00_13680"
     CDS             5693..6664
                     /locus_tag="VFR00_13680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004689000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HEU0160366.1"
                     /translation="MPHAVRIHKTGGPEVLQWDSVDVGAPGPGQVRLKQHAVGVNYID
                     IYQRSGLYKLPMPYVAGSEGAGEVTAVGPGVSDFKVGDRGAYAGAAGGYAEERLMPAD
                     RLVKLPDSIDYQTAAAMMLQGMTVRYLLRETYKVGKGTTLLFHAAAGGVGLIACQWAR
                     HLGATVIGTVSTPEKAELAKAHGATHVINYRSEDFVKRTKELTNGQGVDVVYDAVGKD
                     TYPGSLDCLKPLGLWVSFGNASGAITNFDILQLSAKGSLYATRPTLGTYIAKRADLIA
                     NANDLFEVVSKGIVKINVNHTYPLKDAAQAHKDLEARKTTGSIVLLP"
     gene            6733..7437
                     /locus_tag="VFR00_13685"
     CDS             6733..7437
                     /locus_tag="VFR00_13685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009452220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HEU0160367.1"
                     /translation="MAQKPIELYYWPTPNGHKITIFCEEAGVPYQINPVNIGKGDQFK
                     PDFLAIAPNNRMPAIIDPDGPGGKPISIFESGAILQYLGRKTGQFYPSEERARVEVEQ
                     WLYWQMANLGPNCGQAHHFRNYAQEKLAYPIERYTNEVNRLYGVMDTRLKDREFLAGA
                     YSIADMASYPWVRPYKNQGQDLAEFPNLEKWFQRMHDRPAVAKAVKIGEELRANYNLA
                     TDKDAQKVLFGQRARR"
     gene            complement(7525..>8028)
                     /locus_tag="VFR00_13690"
     CDS             complement(7525..>8028)
                     /locus_tag="VFR00_13690"
                     /inference="COORDINATES: protein motif:HMM:NF012618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0160368.1"
                     /translation="EAGESVSDLVAAARSRPLLVANGPDHMIRVWRTEPRGIGRTYRG
                     PLAGVSALDIAAGGRLVASGGIDGSISVWSTSSSRLQRSFRAHNGRVTALAFMPSERA
                     LLATAGTDGLVKVWDLRAMRNVRVLHGHAGPVRALGFADAGRRLISAGDDGVILLWST
                     LSQASSN"
BASE COUNT         1394 a         2700 c         2477 g         1457 t
ORIGIN      
        1 gtgtagggcc gtcttgagac gctcgacgat ctgggaacgt gcctggggca tgggcctttc
       61 tcccatggct agaatgggtg cctgaggtgt taacggcagg gacctttctg agttccgcac
      121 atttcctccg gcaccagtca gcgcgaaggg cagcgcttgg cccccttctc ggtcccgcga
      181 acgacgctac gcacaccgcg agcgaaaccg cgccagcgtc gcgtgcctta ccaaggctcg
      241 atcaggagga acaatgtcgg ggtcatggcc gttcagccag ccaacgtcgt gatggggatt
      301 ggcgccacga cgtcctaacg ggcaacctga cttccgacaa tcctcagccg tcggcacaat
      361 ggaggagacc aacgatcgcg cataggtttg tccagcgcgt ctgcagcccc ccgagcgacc
      421 agcaggcggc cgcgggcgcc tcgcccgggg tgccgaagac gcacgccctt ttggcacctg
      481 gccgccgcac ggcgccccgc ccaagccgtg ccgccccggc catggccagt tcccccttcg
      541 cgaccccgca ggcgcctcag gggccgacgt aagcggcttc agagccgcag cctcgtgcct
      601 cgcctgcctt gccacccgca gggattgccg ttatacgctc ccctgttttg ccagggccgc
      661 ggcattctgg gcgcgtagct cagcggtaga gcactacctt gacatggtag gggtcacagg
      721 ttcgatccct gtcgcgccca ccatcagcgg ccgaccgaga gacactgggt atgaaagtca
      781 ggaattcgct caagtcgttg cgtggccgcc accgcgccaa tcgcctggtc cgccgcaagg
      841 gccgcgtcta cattatcaat aagaccaacc gccgttttaa ggcccgccag ggctgacggg
      901 cgcgataagc ggctttgacg gcggcgaggc cccccggcta tgttgcgcct atggctgcca
      961 tgatgctgcg cctgacatct tttgttgctg ccgtcgcggt cggcgccagc ctgctcggcg
     1021 gcacgcccgg cagctgggcc cagacccaac ccacgcctcc gcagaccccc aatcccaagg
     1081 agggcacgcc gcccgccatc acgccgccgg cgccgggggc caaacgcggc tccgcggcac
     1141 tcccgaaaac gccggaggag cgcgagaaga cactgtccga tctttacgcg ctgctcgcca
     1201 cggccgacga tgaggacagc gccaaggcga tcgcggaggc catcgagcgc atctggctgc
     1261 attccggcag cgcgaccatc gacctgttga tggagcgctc gatgaaggcc gtctccgaga
     1321 agaatcagga gctcgccttg aagttgctcg accacatcgt tgagcttgca ccggacttca
     1381 cggaggcctg gaaccgtcgc gcctatgtgc acttcgtgcg caacgaaatc gaactcgccc
     1441 tgggggatct gcggcgcgct ttggcgctcg atcccaatca cttcaaggcg ctcgatgggc
     1501 taggtcaaat cttgcgcgag atcggccaaa agaaggccgc cctgcaggcc ttcaagcagt
     1561 tgctcgaggt ccaccccttc tggtcaggcg cgcagcaatt gatcgacgaa ctggcccgcg
     1621 atgttgaagg tcagggcatc taactctctc tgatcgctct ttttttgcgg gcggcagccg
     1681 tcccgccccc tgcaggcggc gggtccaggc ggcatggctt aaaaaaagca gagcgcgcgc
     1741 ctgctgccca gggcggcggc ccacggcgcg tccggcggcg cacgcagatg ttgacaacca
     1801 cgcctggcgc tgggttgtgg ccaacccggc gctggcgatc cgagcatcgt cttgctaagc
     1861 acgcatcttc cgatttgcca ccgtggaggc gcggtgctga gaatcgatta cctctcgatc
     1921 gggggcttgc cgccgctctc cttcgaggtc gcgccgggac aatgcctcgt gatcgagggg
     1981 ccctcgggat cgggcaagac ccgcctgctg cgcgcgattg ccgatctcga cccggcggaa
     2041 ggatacgttt tccttgaagg cgtcgaacgc tcggaaatgc cggcatcgct ctggcgccgg
     2101 cgcgtgcgct ttgtctctgc cgaacctgcc tggtgggcgg cgacgcctcg tgcccacttt
     2161 tccccggcag ccaagctcga tcgcctgctt gcggcgctgg gcctcgaaca tttgaacctc
     2221 gatcagcccg tggcggaact ctccacgggc gagcgccagc gcctggcact ggtgcgcgcg
     2281 cttgccgatg atccgcccgt gttattgctc gatgagccaa cctcggcgct cgcggccacc
     2341 aatgcggcgc ttaccagcga gttcatcaag tttcagatcc ttgccgggcg catcgtcatt
     2401 ttggtcagcc acgacgcggt acaaatcgag cgcctggcga gcgcacgcct acacctggcg
     2461 gatctcgagt ggccgcacgc ccctgagagt gccgcggcat gagccttccc ctcgccaaca
     2521 ccgatctgtt gtgggcggct ctcctcatcg cgctcaatgg cgcgatctcg ctgacgtttg
     2581 ggctacgtct tgagctcagc ctcgccctca tcgccctgcg catggtagtg caagtcgccg
     2641 ccctcggcgt cgtgttgcgc ttcgtgtttc tgcagtcttc accgctgtgg accgccgccg
     2701 tcgccctctt gatgctgctg atcgctggct atgaactcgc cgagcgtcag gagcggcgct
     2761 tcaggggctg gtgggcctac gggctcggca atgcgacgtt gctgttcgcg agcgggctgg
     2821 ccacgctcta tgccgtgacg gtggtgatgg cgccgagccc ttggtacgcg ccgcgctact
     2881 tgttacccgt ccttggcatg gtgctcggca gcacgctcac cagcgtcagt ctggtgctgc
     2941 aaaccttggc cgagggcatc gagcgcgagc gcggcgccat cgaggcgcgc atcgctcagg
     3001 ggggcacgcg ctttgaggcg ttcgctccct tgatgcgccg agcgatgcgc acagccatta
     3061 cgcctctgct caacatcatg agcgtcgccg gtatggtcac gctgcccggc atgatgaccg
     3121 gacagatcct cgccggcgca gatcccgtcg aggcggcgcg ttatcagatc gtgctgatgt
     3181 tcattttggc cggcgcggca gggctcggcg catttgctac cgcggtcggt ggcgttctca
     3241 tgatcagcga tcgccgccat cgcgtgcgcc tcgatcgcct gacatccttg gcttcctggc
     3301 ggccttttta gccgagccga tcgagccacc acttcatacc cgccgagccc ggcgccaaca
     3361 gcacgtaggc ccacaggaat gccgagcccc agttggcgat ctgaaagcgc aaccagggca
     3421 tttcgatggt accggcaacg atcggcacgc tggcccgcag cggcccggaa aaacgtccca
     3481 gcacgatcgc ccacacgccc caacgcttga agaaggcgcg cccgttctcg atcagcttcg
     3541 gatgattttt taagggccac atcttctgga tctgctcgtg atagtggtta ccgagccaat
     3601 aggagaccca gtcgccgagc gcggcaccgg tcgcagccga ggtgacgatc gaccagaaga
     3661 tcagcgggtt ggcggcggcg acaatggccc cgatgccaac cagcatggcc cagaacggca
     3721 ggaccaatga cacaaaagcc aaagactcgc cgaaagccag aataaagacg atggcggaag
     3781 cccagttggg gtgcgcgcgt acgaactcca gcacgacgtt ggtgatatcg gcgagcccca
     3841 tcagttccct cccacgccgc gctggcgtgc ggcgaacaag ccctgatcga ccgaactttg
     3901 tcaacgcgcg ctagggcgca gccccaggtc ggcgcagttt cgcatttcac caacgcctgc
     3961 cgccggcggc acaatttgtg cttggctcgc cttgatctca tggtttgcgt ccggttaata
     4021 gacttcggcc agcggtgttt ggggggggcg gttgtgggcg ttgttgctgt cgccggcgtg
     4081 cgtcacaggg caaatcgccg cctggcgttg ctggtattgt gcggtgccaa tttggcactc
     4141 gtggtcgcca tcgccggcag tatgacggtc ctgtccgacg tgctggcgcc gttgaccggt
     4201 cattttatgg gtgtggggct tgccgctagt ctggggctgc tgacgcgccg cctttgcgcc
     4261 ctcgttcttg gcatcttggc gaccttcctc gtgcatgcgt ggctcggcct ggcaggttgt
     4321 tgccatccac cgcaaccggg gatggtcacg ggcggcgggc tcagcacgat cgccgcagcg
     4381 ggagggtctc acgacctttc ccttgttgcc ctcaacgcct ggcaccacca cggcgagcct
     4441 gcgcgcctct cttcctatct ggccgacgtc gatgccgatc tcgtggtgtt atccgaattc
     4501 gggccgaaca agcgcgccct cctcgaaccg ctcaagagga gccatccctt ccagatcgac
     4561 tgtgccgacg actggtcgtg ttccctggcc ttgctctcgc gcatcccctt cgcctcagca
     4621 ggtacgggtc acattgccga gggcaaacta gcttttgtgt gggcacggct taacggcggc
     4681 gtgaccgtgc tcggtacgca tttgcatcgg ccgagccgag atccgtggtt acacgaacag
     4741 cagatgctcg agctcgttca gttcattgac cgcatccccg gacccgtcat cttggcgggt
     4801 gatcttaaca ccacgccctg gtccaagagc taccgcacgc tgcggcgcgt ggccgggctc
     4861 gtgccggcaa gcacgctgcg accaacctgg cccgcctggc cattggcctt cccacaggtc
     4921 gccctcgacc acatcttcgt ctctcccgat cttgccgtca gtgccgccgg cacggggccg
     4981 gcggttggtt ccgaccatct gccgatttgg gccgtcgtgc agcgccggcc atccttcgac
     5041 cgcagcaagg cggccacgca ccgatttgcc tcaggctttg caccgccgca gccgcatttc
     5101 cgcgcccagc tccttgctga cttcagtggc gaacatgctg gcacgcgaaa tctgcgccgg
     5161 tgaaagcgcc ttgccctcgg ggccgcgcaa ggccttcgga gcattatccg cctcgcgttt
     5221 gccgtacgcc aactcttcgc agctcaattc ctttttgcgg ctgcggaagg caaacaggcg
     5281 cacgcccgaa gcatgcgcct cgggcgtcgc gggctccctc acccacttgc gatctttgtc
     5341 ggcgatcatg gacttgagcg ccacctgacg ctgttcgacg cactcaggcg aatcgtcgag
     5401 gcacgcgagc cctgcccgac cgtcgtcggg cccaaacaac cctgagcagc cggtgctgat
     5461 caaggcgagc gcgctgagcg cgccgagcac cgactgtttg gacaggcggc caatagctaa
     5521 aggcatcgtt tcctcttgag gttcccgcac ggagcccata gaaggcaggc atggcggggg
     5581 tgtggcaagc gcttgcctgt gccccgccct ggcgcctctc agcttgctac gagattcgcc
     5641 gagcccttga atacggccag caacatcacc tcgtcacacg gagtaacggc ccatgcccca
     5701 cgctgttcgc atccacaaaa ccggcggtcc ggaggtgttg caatgggata gcgtcgacgt
     5761 tggtgcaccg ggaccgggtc aggtgcggct caaacaacac gcggtcggcg tcaactacat
     5821 cgatatttat caacgctccg gcctctataa gctgcccatg ccctatgtgg cgggctcgga
     5881 gggtgccggc gaggtgactg cggtcggtcc gggcgtcagc gacttcaagg tcggcgatcg
     5941 tggtgcctac gcgggcgcgg cgggtggcta tgccgaggag cgcctgatgc cggccgatcg
     6001 gctcgtcaag ttgccggata gcatcgacta ccagactgct gccgccatga tgttacaggg
     6061 tatgacggtg cggtatctgc tgcgcgagac ctataaggtt ggcaagggca ccaccctgtt
     6121 gtttcacgct gcggccggcg gcgtggggtt gatcgcctgc caatgggcgc gtcacctcgg
     6181 cgccaccgtc attggcacgg tcagcacccc cgagaaggcc gagctcgcca aggcccatgg
     6241 cgcaacgcac gtgatcaact acagaagcga agattttgtc aaacgcacca aagagctgac
     6301 caacgggcag ggcgtcgatg tggtctacga tgccgtcggc aaagatactt atccaggctc
     6361 cctcgattgt ctgaagcccc tcggcctgtg ggtcagtttt ggcaacgcct cgggcgccat
     6421 caccaacttc gacatcctgc aactatcggc caagggctcg ctctacgcca cgcgccccac
     6481 gctcggcacc tacatcgcca agcgtgccga tctcattgcc aacgccaatg atctgttcga
     6541 ggtagtgagc aagggaatcg tgaaaatcaa cgtcaatcat acctatccgc tgaaggacgc
     6601 tgcgcaggcc cacaaggacc tggaagcgcg caagaccacc ggctccatcg tgcttctgcc
     6661 ctaacgcagt cggggcgggc gccggtcgcg cccgtctttt caaaaaccct caagcttcgc
     6721 gaggagtttc gcatggcgca aaagccgatc gagctctact actggccgac accgaacggg
     6781 cacaagatta cgatcttctg cgaggaggcg ggcgttccct accagatcaa ccccgtcaac
     6841 atcggcaaag gtgatcagtt caagcccgac ttcttggcga tcgcgccaaa caaccgcatg
     6901 ccggcgatca tcgatcccga cgggcctggc ggcaaaccga tctcgatctt tgagtcaggg
     6961 gcgatcctgc agtacctcgg ccgcaagacc ggccaatttt acccgagcga ggagcgtgcc
     7021 cgcgtcgagg tcgaacagtg gctctattgg cagatggcca atctcggtcc caactgcggc
     7081 caagctcatc atttccgcaa ctacgcgcag gagaagcttg cctacccgat cgaacgctac
     7141 accaacgagg tcaaccggct ctacggcgtg atggacacgc gcctgaagga tcgcgagttt
     7201 ctcgccggcg cttattcgat tgccgacatg gcgagctatc cttgggtacg gccgtataag
     7261 aatcagggtc aggatttggc cgagtttcca aacctggaga agtggtttca gcgcatgcac
     7321 gaccgccccg cggtcgccaa ggccgtcaaa atcggtgagg agctgcgcgc caactacaac
     7381 cttgccaccg acaaggacgc gcaaaaggtc ctgttcggtc aacgcgcccg gcgctagcca
     7441 agtcgctgcc agggccaaag ccgtcgcgag gtcctcgcaa cgagccttgc gcctcaccca
     7501 ccagggtgcg catcgcgcgc gccgtcagtt gctactcgcc tggctcagcg tgctccaaag
     7561 caaaatgacg ccgtcgtctc ccgccgaaat caggcgacgg ccggcatcgg cgaagccgag
     7621 cgcgcgtacc ggccccgcat gtccgtgtaa gacccgcaca ttgcgcatag cgcgcaggtc
     7681 ccacaccttg accaaaccgt cggtgccggc cgtggccagg agcgcacgct cgcttggcat
     7741 gaacgccagc gcggtaacac ggccgttatg ggcgcggaag gagcgttgca ggcgcgagga
     7801 cgaggtcgac cacacgctga tggagccgtc tatgcccccg ctcgccacca gtcgcccgcc
     7861 cgcagcaatg tcgagcgcgc tcacgcccgc cagcgggccg cgataggtac gaccgatacc
     7921 gcgcggctcg gtgcgccaca ccctgatcat gtggtcgggt ccgttggcaa ccaacagggg
     7981 acgcgaccgg gcggcggcca ccaggtcgga gacgctctcg cccgcctc
//
LOCUS       DASTMT010000599         3891 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_4814236, whole genome shotgun sequence.
ACCESSION   DASTMT010000599 DASTMT010000000
VERSION     DASTMT010000599.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3891)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3891)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3891
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4814236"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..214)
                     /locus_tag="VFR00_13695"
     CDS             complement(<1..214)
                     /locus_tag="VFR00_13695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007603988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing protein"
                     /protein_id="HEU0160369.1"
                     /translation="MSAKLGGGQHAPQHHHVGPRPGQPVAAPARAPVKDPVCGMSVDP
                     QNARHSFEHAGQTWYFCSARCRERFAE"
     gene            330..605
                     /locus_tag="VFR00_13700"
     CDS             330..605
                     /locus_tag="VFR00_13700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006022124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HEU0160370.1"
                     /translation="MGQKAKSPCLTRLARIEGQVRGLSRMIEEDRYCIDIVTQIAAVR
                     AALRRVEEEVLRDHIAHCVEHAIVSGNTAEQRRLVTELMDVLGRSAR"
     gene            630..1112
                     /locus_tag="VFR00_13705"
     CDS             630..1112
                     /locus_tag="VFR00_13705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010398620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF411 domain-containing protein"
                     /protein_id="HEU0160371.1"
                     /translation="MVSGRGPSRRTVLQLLGAAAALNLGEVRAEGAPEVKVWKDPNCG
                     CCTGWVEHLRKSGFSVAVVEQVDLSSIKAEAGVPAELASCHTARIDGYVLEGHVPAAA
                     IRRLIKERPEAIGLAVPGMPIGSPGMEGGTPEVYAVILFGKAQRIVYGRFLGTEARAE
                     "
     gene            complement(1163..2347)
                     /gene="chrA"
                     /locus_tag="VFR00_13710"
     CDS             complement(1163..2347)
                     /gene="chrA"
                     /locus_tag="VFR00_13710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011982975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HEU0160372.1"
                     /translation="MRDPEQGTPWEVFAVFLRLGLTSFGGPVAHLGYFQQEFVVRRRW
                     LTQRSYADLVALCQFLPGPASSQVGLAIGLSRAGYGGALAAWAGFTLPSALLLILFAY
                     SVVGLESLIGTGWLSGLKAAAVAVVALAVASMARSLAPDRSRATLAVAAAILALSIPS
                     TWGQLAAIALGGLVGLALEPEVSPGEDAALPLSVRPGVAAIALLAFFGLLLLLPLAQT
                     VVASPGLAQFTAFYRAGALVFGGGHVVLPLLQAEVVPTGWVSHDAFLAGYGAAQAVPG
                     PLFTFAAYLGAVIGGWGMALVCLIAVFLPSFLLVIGALPFWEALRRTPRIQAALKGVN
                     AAVVGLLLAALYNPVWTAGITSARDFALALTAFLLLFMWQWPPWLVVVLSAAAGLATA
                     QL"
     gene            complement(2402..2815)
                     /locus_tag="VFR00_13715"
     CDS             complement(2402..2815)
                     /locus_tag="VFR00_13715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c family protein"
                     /protein_id="HEU0160373.1"
                     /translation="MGRASSLFAGILLAVTGALLFSAAAAEEGNATRGERAFQRCFAC
                     HSVDPAEPAKLQGPSLFKIIGRPAAAVPGFVYSEAMQRAAAAGLVWDAATLDRFLADP
                     DAVVAGTAMSIAPVRDADERADLVVYLKRSGAFGP"
     gene            complement(2827..3783)
                     /locus_tag="VFR00_13720"
     CDS             complement(2827..3783)
                     /locus_tag="VFR00_13720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018458065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase"
                     /protein_id="HEU0160374.1"
                     /translation="MRSTLFALILVLALSTSAGAHESKYYDLAKGDYPHDVAISAKGE
                     VWFAGQKAGIAGRLDPVSGSIERIALGKNSAPHGVIIGPDGAPWFTDGGQNAIVRVDP
                     ASKAVKVWPLPPERMPYTNLNTAAFDGRGRIWFTGQNGIYGRLDPSSGDMKVWDAPKG
                     RGPYGITGTPKGDIWFVSLAGNYLANVDLESGAATVYEPPTKEQGARRVWSDSKGRLW
                     ISEWNSGNVSVYDPALKRWQQWKLPGASPRTYAVWVDPDDKVWLTEWSSNAVVRFDPE
                     TETFAAFPSDRPGANVRQLLGRKGEAWIAESGTERIRVIRTP"
BASE COUNT          705 a         1250 c         1342 g          594 t
ORIGIN      
        1 cctcggcgaa gcgctcgcga cagcgcgccg agcagaagta ccaggtctgc cccgcgtgct
       61 cgaagctatg gcgcgcgttt tgcgggtcga cgctcatgcc gcacacgggg tccttgacgg
      121 gtgcgcgggc cggcgcggca acgggctggc ccggacgcgg accgacgtga tggtgctgcg
      181 gggcatgctg accgccaccg agcttcgcgc tcatgactgc taactcctgg ctcagctgct
      241 ggctgtcttg gccggcggtc cgcgcgtatt ctggctcaga cccgcttttc gccttgacca
      301 tatataccct gtgggggtat cgagcaagga tgggtcagaa agcaaaatcg ccttgcctca
      361 cccgccttgc ccgcatcgag gggcaagtgc gcgggctgag ccgcatgatc gaggaggatc
      421 gctactgcat cgatatcgtg acccagatcg cggccgtgcg cgcggcgctg cgccgggtcg
      481 aagaagaggt gctgcgcgac cacattgctc actgcgtcga gcatgccatt gtcagcggta
      541 atacggccga gcaacgacgt ctcgtgaccg agctgatgga cgttttgggt cgcagcgcgc
      601 gctgaagctg tccagtcaag gagagacgta tggtttcggg tcgaggacca tcacggcgga
      661 ccgtgctgca attgctcggc gccgcggccg cgttgaacct cggcgaggtt cgcgccgagg
      721 gcgcgcccga ggtcaaggtc tggaaggatc cgaactgcgg gtgctgcacg ggctgggtcg
      781 agcatctgcg caaaagcgga ttttccgttg ccgtggtcga gcaggttgat cttagcagca
      841 tcaaggccga ggccggcgtc ccggccgagc ttgcctcctg tcacaccgcc cgcatcgacg
      901 gctatgtcct ggaaggccac gtccccgccg cggctattcg gcggctgatc aaggaacggc
      961 cggaggcgat cgggctcgcc gtgcccggca tgcccatcgg ctcgccgggg atggagggcg
     1021 gcacccccga ggtctatgcc gtgatcctgt tcggcaaagc ccaacgcatc gtctacgggc
     1081 ggtttctcgg cacggaagca agagccgagt gagaggcgct gcgcgccccc ggcacggcct
     1141 attccgactt ccctgcgtct tatcagagct gcgccgtggc gagacctgcg gcggcgctca
     1201 ggaccacgac cagccagggc ggccactgcc acataaaaag aagcaggaag gcggtcagcg
     1261 ccaaggcgaa gtcgcgcgcg ctggtgatgc cggcggtcca aaccggattg tacagtgctg
     1321 ccaacagcag tccgactacg gcggcgttga cgcccttgag cgcggcctgg atcctgggcg
     1381 tccggcgcag ggcctcccag aaggggagcg cgccgatgac gaggaggaag gagggcaaaa
     1441 acacggcaat caagcacacc agcgccatgc cccagccacc gatgacggcg ccgagatagg
     1501 ccgcaaaggt gaagaggggt cccgggacgg cctgcgccgc gccatagccg gcgaggaagg
     1561 catcgtggct cacccagccc gtcggcacga cctcggcttg cagcaacggc agtaccacgt
     1621 ggcccccgcc gaacaccagc gcgccggcac gatagaaggc ggtgaactgc gccagcccgg
     1681 ggctcgcgac cactgtttgc gccaggggca gcagcagcaa gaggccaaaa aaggcgagaa
     1741 gcgcgatagc tgctacgccc gggcgcacgg aaaggggcag ggccgcatct tcgcctgggc
     1801 tgacttcggg ctcgagtgcc aggccgacga ggccgccgag cgcgatggcg gcgagctggc
     1861 cccaggtcga gggaatgctc aaggcgagga tcgccgccgc cacggcgagg gtggcgcggg
     1921 accggtcggg ggcgaggctg cgcgccatgc tggcgacggc gagggcgacg acggccaccg
     1981 ccgcggcctt gaggccactg agccaacccg tgccgatcag actttcgagg ccgacgacgc
     2041 tataggcaaa gagaatgagg agcagggcgg agggcaacgt gaacccggcc caggccgcca
     2101 gcgcacctcc atagccggcg cgcgacaagc cgatggcgag gccgacttgg ctgctcgccg
     2161 gcccgggcag gaattggcag agcgcgacga gatccgcata ggaacgttgg gtcagccagc
     2221 gtcggcggac cacaaattcc tgctggaaat agccaaggtg cgcgacgggc ccgccgaacg
     2281 acgtcagacc aagtctgagg aacacggcga agacttccca gggggtgccc tgctcggggt
     2341 cgcgcacgag atttgcctcc ctttccctca cacccgtctc gcttgctgcg gcggctttag
     2401 atcatgggcc aaaggcaccg gagcgcttga gatagaccac cagatcggcg cgctcgtcgg
     2461 catcgcgcac tggggcgatg ctcatggccg tgccggccac cacagcgtcg ggatctgcca
     2521 agaagcggtc aagcgtcgcc gcatcccaga cgagaccggc cgcggcggcg cgttgcatgg
     2581 cctcggaata gacaaagccg ggaacggcgg ccgcgggtcg gccgatgatt ttaaagaggc
     2641 tgggtccctg cagcttcgcc ggctcggcag ggtcgacgga gtgacaggca aagcagcgtt
     2701 gaaaggcgcg ctccccgcgc gtggcgttgc cctcctcggc cgcagcggct gagaacagta
     2761 gagcgccggt gaccgccagg agaatgccag caaacaggga cgatgctcgg cccacgatca
     2821 gagcttctaa ggcgtgcgaa tgacgcggat gcgctcggtg ccggattcgg caatccaggc
     2881 ttcgcctttg cggccgagca attggcgcac gttggcgccc gggcgatcgc tggggaaggc
     2941 cgcaaaggtt tccgtttccg gatcgaagcg cacgacggcg ttgctgctcc actccgtcag
     3001 ccacaccttg tcatcgggat cgacccagac ggcataggtc cgtggcgatg cccccggtag
     3061 cttccattgc tgccagcgct tgagggctgg atcgtagacg ctgacgttgc ccgagttcca
     3121 ttcactgatc caaagccggc ccttggagtc cgaccacacc cggcgtgcac cctgctcttt
     3181 ggtgggcggt tcataaacgg tggcggcccc gctctccagg tcgacgttgg ccagataatt
     3241 gccggcgagc gaaacgaacc agatgtcgcc cttgggcgtc ccggtgatgc cataggggcc
     3301 gcgccccttg ggtgcgtccc acaccttcat atcccccgag ctggggtcga gccggccata
     3361 gatgccgttc tgtccggtga accagatacg gccgcggccg tcgaaggcag ccgtgttgag
     3421 gttggtgtag ggcatgcgct ccggcggcag cggccacacc ttgaccgcct tgctggcggg
     3481 atcgacgcgc acgatggcgt tctggccgcc gtcggtaaac cagggggcgc cgtccggccc
     3541 gatgatgacc ccgtgcggtg ccgaattctt gccgagtgca atacgttcga tgctgccgct
     3601 caccgggtcg aggcggccgg cgatgccggc cttttgcccg gcaaaccaga cctcgccctt
     3661 ggccgagatg gcgacgtcat ggggatagtc ccccttggcg agatcataat acttactctc
     3721 gtgcgcgccg gcggaggtcg aaagcgccag cacaaggatc agagcaaaaa gggtggaccg
     3781 catgctaacc tccgtcatcc cgagccggga gccacatctt acagcaagcc cggccgcgcc
     3841 atttggggaa cgccaccatg ctgcaaaaaa gcattctttt gctacttgcc t
//
LOCUS       DASTMT010000600         8661 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_6427307, whole genome shotgun sequence.
ACCESSION   DASTMT010000600 DASTMT010000000
VERSION     DASTMT010000600.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 8661)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8661)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8661
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6427307"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..542
                     /locus_tag="VFR00_13725"
     CDS             <1..542
                     /locus_tag="VFR00_13725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015667774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HEU0160375.1"
                     /translation="EAKALEKDGVDVIQFDEPAFNVFMDEVNDWGIAALERAAAGLAS
                     TTAVHICYGYGIKANIDWKSSLGREWRQYEAIFPALAKSRIQQVSLECRNSKVPVSLL
                     GLLKGKQILVGAIDVASDTVETPEEVAAVAAEAAKHIPAEAIQLCTNCGMAPMRRDIA
                     FAKLSALAAGAALARQRFA"
     gene            complement(563..1087)
                     /locus_tag="VFR00_13730"
     CDS             complement(563..1087)
                     /locus_tag="VFR00_13730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018316832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU0160376.1"
                     /translation="MNETASRLRPLADHRPARAVPAATRIGHVHLKVADLERALSFYC
                     DVLGFEVTQRRQGAVFISAGGYHHHIALNTWESLGGTAPPPGSTGLYHLAILYPTRRD
                     LADALRRVMAAGIELDGASDHGVSEALYLRDYDQNGVELYWDKPRERWPVTPTGELSM
                     ISARLDLRSLLAEQ"
     gene            complement(1116..1862)
                     /locus_tag="VFR00_13735"
     CDS             complement(1116..1862)
                     /locus_tag="VFR00_13735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020083931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HEU0160377.1"
                     /translation="MTALPAAQRSFERTQVWIFDLDNTLYPMECNLFGQVDRRMGEFI
                     ARYLGVPVEYARHLQKTYYRQFGTTLCGLMRVHGMDPKPFLDYVHDLDLSGLAEHPEL
                     AARIARLPGRKLIFTNGSRAHAERVAGKVGILHLFDGIYDICDAGYVPKPSPACYEHF
                     LKVHGVSATLSAMFEDIPGNLEAPHALGMTTVLVHAAGNYDHPVQQKIRAWLEPPAHI
                     HYMTDNLAAFLASSTVGALEPSAARGEPRK"
     gene            complement(1863..2798)
                     /gene="argB"
                     /locus_tag="VFR00_13740"
     CDS             complement(1863..2798)
                     /gene="argB"
                     /locus_tag="VFR00_13740"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020083930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HEU0160378.1"
                     /translation="MAKFQADTKDSAAPASAHTQARILAEALPHMQRYDRQTVVVKYG
                     GHAMGEPALAAAFAQDIVLLKQAGVKPIVVHGGGPQIAGMLKRLQLKSEFVHGLRVTD
                     KPTVEVVEMVLAGLINKDIVTAINRQGGKAVGISGKDANLMIARKITQMPDPQSNLMQ
                     AVDIGYVGDPAEVNPHIVDVISASDLIPVIAPVGISRDGETLNINADTFASALAARMK
                     AKRLLLLTDVAGVLDKDNRLIAELSVAQARELIRGGIISGGMIPKIEGCIEVVEAGVE
                     AVVVLDGRVPHCVLLELFTQHGVGTLVSRKPTKAA"
     gene            3029..4885
                     /locus_tag="VFR00_13745"
     CDS             3029..4885
                     /locus_tag="VFR00_13745"
                     /inference="COORDINATES: protein motif:HMM:NF014862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HEU0160379.1"
                     /translation="MPQVFAEEFKSFPISAELGESLERAHRFAREQSHRLVTLEHLLL
                     ALTEDADATAMLASASVDLGRLSGDVSGYLGGLLEDMRGEAHTELHPDGDLIRVLKAA
                     TTAAQQSRRRHIDGAIVLAAVVGDAKSAAAGLLKALGLTFEGAIRALQRANQPARGKA
                     PQPNSAAHWGSGASAGKPGTAAGLEQQPPPGAGVAMPPASPEISLATSAEEFLAAARA
                     RIQQRAAAARATEPPAAGPLGAAAPSGITTGERPAPSSAPPSNRGDLNPSSLAAAIRD
                     VAGAAPAEPAAAAPAAPAPEPPPPARAGEAAAPAPSPPEPRAPADSAAALAPRRTPAP
                     APEAAAVARLPQPGPHRSAGPLPGRPLRAGDGAPRPPLPSAPPHRPPAGFGPPPRPVR
                     APFAANGAGAPGLPQARPPGAPMPPHDANAGIVVPLQPRGGPTGPANYSERGPLAESV
                     PRRMRSGVAASAEVRIARDKIDSLMLALCNRGVRPEAYPTRTLAVRLRAPNGGFAIEA
                     TAPETQWIDRAMGGPGDGFAAWRWTITPERHGRNRLLLTVSARLIGADGLVAEAAPSD
                     RSIEVKIAPNYRRLAQRWAGWLLAMLAGVALGQFGGEVWASLVFLIRQATGT"
     gene            complement(4932..5594)
                     /gene="yihA"
                     /locus_tag="VFR00_13750"
     CDS             complement(4932..5594)
                     /gene="yihA"
                     /locus_tag="VFR00_13750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020083928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HEU0160380.1"
                     /translation="MTVLGFTPEQLAAGEALFRRPWQFVKSVPHLAALPAGEHCEICV
                     VGRSNVGKSSLINGLVGRTLARTSNTPGRTQEANFYEAPGAALFLVDLPGYGFAEAPK
                     ARVEAWTRLVRDYLRGRARLVRAFLLLDARRGLTPADLEMMRRLDEAAVSYQAVLTKI
                     DKIKPTELARVLAATEDALRRHPAAAAGLLATSAVTGAGLAELKAQIAQLAAASGAGN
                     LV"
     gene            complement(5641..7764)
                     /gene="yidC"
                     /locus_tag="VFR00_13755"
     CDS             complement(5641..7764)
                     /gene="yidC"
                     /locus_tag="VFR00_13755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEU0160381.1"
                     /translation="MPQDQDHQKNLLLAIVLSVAVLLAWQMFYAGPKLKDEQERRQRA
                     QQEQTQAKPSAAPTTTPGEAPQVAAPSTAAPTEAQNREAILRSSAQVRVPIETPSLQG
                     SIALQGGRIDDLVLLKYRETVAPQSPNVVLFSPSGAPAPYYAEYGWVAGPGVTQPMPG
                     NDTVWRLEKGNALTPSSPVTLLWDNGQGLIFRRTVAADDNYLLTVTDTVENRSGAELS
                     LYPYALISRHGLPHTQGFYILHEGPIGVMGEEGLKELHYADLLKEGGNKTFKNTGGWI
                     GITDKYWAAALIPDQKVAYTASFRGIKGSKDRFQTDLLLSAIPLAPGASASTTSNLFA
                     GAKQVTLIEAYKEQLGAKQFDLLIDWGWFYFITKPLFKLLHWLSQLLGNYGLAILATT
                     VLVKLAFFPLANKSYESMAKMKKLQPEMEKIRERFKDDRVKQQQELMGLYKNQKINPM
                     SGCLPIVLQIPVFFALYKVLFVTIDMRHAPFFGWIKDLSAPDPTSLFNLFGLLPYQVP
                     EFLHIGAWPLIMGATMWFQMQLNPQQPDPVQQKIFNWMPVIFTFMLASFPSGLVIYWA
                     WNNVLSLLQQYAIMRKNDTEIHLWKNLGIEKWKGRVEAVKAYGTQRLGRAADVGSSKP
                     TPANPPQRGPHAKPRAANLLPEAPMTREQALRTLGLDTDATAAEIELALAKATGKQGG
                     LNGSDHAIAAKIDEARHILSGKEGR"
     gene            complement(7822..8214)
                     /gene="rnpA"
                     /locus_tag="VFR00_13760"
     CDS             complement(7822..8214)
                     /gene="rnpA"
                     /locus_tag="VFR00_13760"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEU0160382.1"
                     /translation="MAAAGAEPPISAGRLATLKCRKEFQAVRKGSRWSTRVFVLEGRQ
                     RTAAPGPAQPRFGFTVARAVGGAVERNRVRRRLKAAVRSVQKTHARDDFDYVVIARRA
                     VLEAEFADLVRELYKALDKVHRPRPRTT"
     gene            complement(8241..8375)
                     /gene="rpmH"
                     /locus_tag="VFR00_13765"
     CDS             complement(8241..8375)
                     /gene="rpmH"
                     /locus_tag="VFR00_13765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010659431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEU0160383.1"
                     /translation="MKRTYQPSKLVRKRRHGFRKRMLTTGGRRVVARRRAKGRKRLSA
                     "
BASE COUNT         1450 a         2882 c         2823 g         1506 t
ORIGIN      
        1 aggaggccaa agcactcgag aaagacgggg tcgatgtcat ccaattcgac gagcccgcct
       61 tcaacgtgtt catggacgag gtgaacgact ggggcatcgc cgccctcgag cgggcggcgg
      121 cgggcctcgc cagtaccacg gccgtgcaca tctgctacgg ctacggcatc aaagccaaca
      181 tcgattggaa gtcatcgctg ggccgggaat ggcgtcagta cgaggcgatt ttccccgccc
      241 tcgccaagag ccgaattcag caggtgtcgc tggaatgccg caactccaag gttcccgtca
      301 gcctcctcgg cctactcaag ggaaaacaga ttctggtcgg tgcaatcgac gtcgccagcg
      361 acaccgtcga gacgcccgaa gaggtcgccg cggtcgcggc ggaggccgcc aagcacattc
      421 cagccgaggc gatccagctg tgcaccaatt gcggtatggc gcccatgcgc cgtgacatcg
      481 ccttcgccaa gctcagcgcc ttggcggcag gcgcggcgct ggctcgccag cgctttgcct
      541 aagccgtcac ggcgccaggt gactattgct cggccaacag gctgcgcaga tccaggcggg
      601 cgctgatcat gctgagttca cccgtcggcg tgaccggcca ccgctcgcgc ggcttatccc
      661 agtagagctc cacgccgttc tggtcatagt cgcgcagata aagggcctcg ctgacgccat
      721 gatcgctcgc gccatcgagc tcgatgccgg ccgccatcac acggcgcagc gcatcggcta
      781 ggtcccgtcg cgtcggatag agaatggcaa gatggtagag cccggtgctg ccgggcggcg
      841 gcgctgtgcc gccgaggctt tcccaggtgt taagcgcgat atggtggtga tacccccccg
      901 cagaaatgaa gacagcgcct tgacgccgct gcgtcacctc aaacccgagc acgtcgcaat
      961 aaaacgataa ggcccgctcc aagtcagcca ccttgaggtg gacgtggccg atccgggttg
     1021 ccgccgggac cgcgcgcgcc ggccgatggt cggccagcgg gcgcagtcgt gacgctgtct
     1081 cgttcatgag cttgctccaa tagcgctatg cgcgcctatt tccttggctc accccgcgcc
     1141 gccgagggct ccagcgcacc tactgtgcta ctcgccagaa acgccgcaag attatcggtc
     1201 atgtagtgaa tgtgcgcggg cggctcaagc caggcgcgga tcttctgctg caccggatga
     1261 tcgtagttgc cggcggcgtg cacgagcacc gtggtcatgc cgagggcgtg cggggcctca
     1321 agattgcccg gtatgtcctc aaacatcgcc gacaaggttg cgctgacgcc gtgcaccttg
     1381 aggaaatgct catagcaggc gggcgacggc tttggtacgt aaccggcatc acaaatatca
     1441 tagatgccat cgaacaggtg caggataccc accttgccgg cgacgcgctc ggcgtgcgca
     1501 cgcgatccgt tggtaaagat cagcttgcga cccggaaggc gcgcgatgcg agccgcgagc
     1561 tcgggatgtt cggcaagccc tgacaggtcg agatcgtgaa cgtagtcgag aaagggcttg
     1621 ggatccatgc cgtgcacccg catcagtccg cacagcgtgg tgccaaactg ccggtaatag
     1681 gtcttctgca ggtgccgcgc gtattctacg ggaacaccga gatagcgcgc gatgaactcg
     1741 cccatgcggc ggtccacttg gccaaacagg ttgcactcca tcggataaag cgtgttgtcg
     1801 agatcgaaga tccacacctg ggtgcgctcg aagctgcgct gtgcggcagg cagagcggtc
     1861 attcatgccg cctttgtcgg cttgcggctg accagcgtgc cgacgccatg ctgggtgaag
     1921 agctccagca gcacgcagtg cggcacgcgt ccatccagca ccacgaccgc ctcgacgccc
     1981 gcttcgacga cctcgataca cccctcgatt ttcgggatca tgccaccgct gatgatgccg
     2041 ccgcggatca gctcgcgcgc ttgcgcaact gaaagctcgg cgatgagcct gttgtccttg
     2101 tcgagcacac cggccacatc ggtcagcaac agcagccgct tggctttcat gcgcgccgcc
     2161 aaggcgctgg cgaaggtgtc ggcgttgatg ttgagggtct cgccgtcgcg gctgatcccg
     2221 accggagcaa tgacgggtat gaggtcggat gcggatatta catcgacgat gtgcgggttg
     2281 acctccgccg gatccccgac atagccgata tcgacggcct gcatcaggtt cgactgagga
     2341 tccggcatct gcgtgatctt gcgcgcgatc atcaagttgg catctttgcc agaaattccg
     2401 accgccttgc caccctgccg attgatcgcg gtcacgatat ccttgttgat gagaccggcg
     2461 agcaccatct cgacgacctc gacggtcggt ttgtcggtaa cccgcaagcc atgcacaaac
     2521 tcggatttca gctgcaaccg cttcagcatg ccagcgatct gcgggcctcc gccatgcacg
     2581 acaattggct tcacgccggc ttgcttgagc aaaacgatat cctgcgcaaa cgccgcggcc
     2641 agcgctggct cgcccatggc gtgtccgccg tacttgacca ccacggtctg ccgatcgtaa
     2701 cgctgcatgt gcggcagggc ctcggccagg atgcgcgctt gcgtgtgcgc cgaggccggc
     2761 gcggcactgt ctttcgtgtc tgcctggaat ttcgccatct gcgggcagcc tcgatgatgc
     2821 cgacttgcct aagcacttat atcggtcatc gtgacggctc aacggaattt gcgccacaga
     2881 cacccaccgg ggctgcacaa aggcggccac ctttgctaaa acccctgggt ctttggctgt
     2941 cattgcgccc gcgccaggtt cggccggcat cgatcgaacc gccgctgggc tttgctcggg
     3001 ggacctgcgc gctgttgggg agtggctgat gccgcaagtc tttgcggagg agttcaaatc
     3061 ctttcccata tctgctgagc tgggcgagag cctggagcgc gcgcaccggt tcgcccggga
     3121 acaatcgcat cggctggtca cgctcgagca tcttttgctg gccctgacgg aggatgccga
     3181 tgcgactgcc atgctcgcct cggcgagcgt cgatctcgga cgtctcagtg gcgacgtctc
     3241 cggctacctg gggggcttgc tggaggacat gcggggtgaa gcccatacgg aacttcatcc
     3301 cgacggggac ctgatccgcg tcctcaaggc ggcaacgacg gccgcccagc agtcccggcg
     3361 gcggcacatt gacggggcca tcgtgctggc cgcagttgtc ggcgacgcga aaagtgccgc
     3421 ggccggcctc ctgaaggcgc tcggcctgac gttcgagggg gccatccgcg ccctgcagcg
     3481 ggccaatcag cccgcgcggg gcaaggcgcc gcagcccaac agcgcggcgc actgggggtc
     3541 cggggcgagc gccggcaaac ccggcacggc agccggcctc gagcagcaac ccccgccggg
     3601 cgcgggggtg gcgatgccgc ccgcgagccc ggaaatttcg ctcgccacct ccgccgagga
     3661 gtttctggcg gcggcacgcg cgcgcattca gcagcgcgcc gcggccgcgc gcgccactga
     3721 gccgcccgcc gccggccccc tcggcgcggc cgcgccctcg gggataacga cgggggaacg
     3781 cccagcgccc agcagcgcgc ccccatccaa ccgcggcgat ctcaacccct cgagcctggc
     3841 ggccgccatc cgcgacgttg ccggcgcagc gccggcagag ccagccgcgg ccgcacccgc
     3901 cgccccggcg cccgagccgc cgccgcccgc acgggccggg gaggcagccg ccccagcgcc
     3961 gagcccaccc gagccgcgcg caccggccga cagcgccgct gccctggcgc cgcggcgaac
     4021 cccagcgccg gcaccggaag ccgccgccgt cgcccggctg ccgcaaccgg ggccccaccg
     4081 cagcgccggg ccgttgcccg ggcggccgct gcgagcgggc gacggggcac cgcggccgcc
     4141 gctcccgtcc gcgccgccgc accggccacc ggcgggcttc ggtccgccgc cgcgccctgt
     4201 gcgcgctcct tttgccgcca acggcgctgg tgcgccgggc ctgccgcagg cacggccgcc
     4261 gggtgcgcca atgcccccgc acgatgcgaa cgccggcatc gtcgtcccgc tgcagccccg
     4321 cggcggcccg actggcccgg cgaactattc ggagcgcggt ccgctggccg agagcgtgcc
     4381 gcggcgaatg cgcagcggcg tggcggccag tgccgaagtg cgcattgcac gcgacaagat
     4441 cgacagcctg atgctggccc tttgcaaccg cggcgtgcgc cccgaagcct atccgacccg
     4501 cacgctcgcc gtgcggctgc gggcgcccaa cggtggcttc gccatcgagg ccaccgcgcc
     4561 ggaaacgcag tggattgacc gggcgatggg gggccccggc gacggttttg cggcttggcg
     4621 ctggaccatt accccggaac ggcacggtcg caatcgcttg ctactgacgg tatcggcgcg
     4681 gctcattggc gctgatggac tagtggccga agcggcgcct tcggaccgct cgatcgaggt
     4741 caagatcgcc cctaactatc gccggctggc ccaacgctgg gcgggctggt tgctggcgat
     4801 gctggccggc gtggcgctcg gtcagttcgg cggcgaggtt tgggcgagcc tcgtttttct
     4861 gatccggcag gccacgggca cttgacggct cgagcaggcg tgagccgccc ctgagcacca
     4921 acttcgacca gtcagacgag gttgccggcg ccactcgcgg ccgccagctg ggcgatctgt
     4981 gccttgagct cggctaggcc ggccccggtc accgccgagg ttgccaagag gccggcggcg
     5041 gcggcgggat gccgtcgcag ggcgtcctcg gtggcggcaa gaacccgcgc gagctcggtg
     5101 ggcttgatct tgtcaatttt cgtcagcacc gcctggtagg atacggccgc ctcgtccagg
     5161 cgccgcatca tctcgaggtc cgccggggtg aggccgcgcc gcgcgtcgag caagaggaag
     5221 gcgcgcacaa gcctcgcccg cccgcgcaaa taatcgcgca cgagcctggt ccaggcctcg
     5281 acgcgcgctt tgggcgcttc ggcaaagccg tagccgggta ggtcgacgag aaacagtgct
     5341 gccccgggcg cctcgtagaa attggcctct tgcgtacggc ccggcgtgtt ggatgtgcgc
     5401 gccaaggtgc gcccgacgag cccgttgatg aggcttgatt tgccgacgtt ggagcggccc
     5461 acgacacaga tctcgcagtg ctcgcccgcg ggcagcgccg ccaagtgcgg cacgctcttg
     5521 acgaattgcc agggacggcg aaacaacgcc tccccggcgg ccagttgctc aggtgtaaag
     5581 ccgaggacgg tcatgcgaca tgccgtgccc tcgcctggcg gcgagcccgg gtgccaaggc
     5641 tcatcgtcct tccttgccgc tcagaatgtg tcgagcctcg tcgatcttcg cggcgatcgc
     5701 atgatcggaa ccgttcaacc ccccctgctt gcccgtcgcc ttggcgagcg cgagctcgat
     5761 ttccgccgcc gttgcgtcgg tgtcgagacc caacgtgcgc agcgcctgct cgcgcgtcat
     5821 cggtgcctcc ggcagcaggt tggccgcgcg cggcttggcg tgagggcccc gctggggcgg
     5881 atttgccggc gtcggtttgc tgctgccgac atctgcggcg cgcccaagcc tttgcgtccc
     5941 ataggctttt accgcctcga cccgcccctt ccacttttcg atgcccagat tcttccaaag
     6001 atggatctcg gtatcgttct tgcgcatgat tgcatactgc tgcagcaacg agagcacgtt
     6061 gttccaggcc caatagatca cgaggcccga gggaaatgag gcaagcatga acgtgaagat
     6121 caccggcatc caattaaaga tcttctgctg cacgggatcg ggctgttgcg ggttgagctg
     6181 catctggaac cacatcgtgg cacccatgat cagcggccag gcgccaatgt gcagaaactc
     6241 cggcacctga tagggaagca agccgaagag attgaagagc gaggtgggat cgggcgcgga
     6301 cagatccttg atccagccaa aaaacggcgc atgccgcata tcgatggtga cgaacagcac
     6361 tttgtagagg gcgaagaaca cggggatctg caggacaatg ggcagacagc ccgacatcgg
     6421 attgattttc tggtttttat agagccccat taactcctgt tgctgcttga cgcgatcgtc
     6481 cttgaagcgc tcccggattt tctccatctc cggctgcagc ttcttcattt tcgccatcga
     6541 ctcgtagctc ttattggcga gcggaaagaa cgcgagcttg acgagcacgg tggtggcaag
     6601 gatggcaagg ccgtagttgc cgagcagttg ggagagccag tgcagcagct tgaacagcgg
     6661 cttggtgata aaatagaacc agccccaatc gatcagcaga tcgaattgct tggcgcccag
     6721 ctgctccttg taggcctcga tcaaggtcac ctgcttggcc ccggcgaaca gattgctcgt
     6781 ggtgctggcc gaggcaccgg gggcgagggg aatggcgctt agcagcagat cggtctggaa
     6841 ccgatctttg ctgcctttga tgccgcggaa actggcggtg taggcgacct tttggtcggg
     6901 gatgagcgct gcggcccagt atttgtcggt gatgccgatc cagccgccgg tattcttgaa
     6961 ggtcttgttg ccgccctcct tgaggagatc ggcatagtgc agctccttca ggccctcctc
     7021 gcccatgacg ccgatcggcc cctcgtgcag gatgtaaaac ccctgcgtgt gcggcaagcc
     7081 gtggcgcgag atcaaggcat aggggtagag actgagctcc gcaccgctgc gattttccac
     7141 ggtatccgtc acggtcagca aatagttgtc atcggcggcg acggtgcgcc ggaaaatgag
     7201 accctggcca ttgtcccaga ggagcgtgac gggcgaggac ggcgttagcg cattgccctt
     7261 ctccagccgc cacaccgtgt cattgccggg catcggctgg gttacgccgg gtccagcgac
     7321 ccaaccgtat tcggcgtaat agggggcggg agccccggag ggagagaaca gcaccacgtt
     7381 ggggctttgc ggcgccaccg tctctcgata cttcagcagc accaaatcgt cgatacgccc
     7441 gccttgcaag gcaatcgagc cctgcaggct tggggtttcg atcggcacgc gcacctgcgc
     7501 ggaggaccgc aaaatggctt cgcgattttg cgcttcggtg ggggcagccg tggacggggc
     7561 cgccacctgc ggggcctcgc ccggtgtggt cgtgggcgcg gcggaaggct tggcctgggt
     7621 ctgttcttgc tgggcgcgct gccgccgctc ttgttcgtcc ttgagtttgg gccctgcata
     7681 aaacatttgc caggccagca gcacggccac cgacagcacg atggcaagca gtaggttttt
     7741 ctggtggtcc tgatcctggg gcatttggct tccgcaaagt gagcttaact gtgcttttct
     7801 tccgctcgcc gcgcgcgacc ctcaggtcgt ccgcggccgc gggcggtgaa ctttatcgag
     7861 ggccttgtag agctcccgaa cgaggtctgc gaattcggcc tccagcaccg cgcgccgcgc
     7921 aatgacgaca taatcgaagt catcccgcgc atgcgtcttc tgcacgcttc tgaccgccgc
     7981 tttcaagcgg cgtcgcaccc ggttgcgctc gaccgcgcca ccgacggcac gggccacggt
     8041 gaagccgaac ctcggctgcg ccggccccgg cgcggccgtc cgctggcgcc cctccagcac
     8101 gaagacccgc gtcgaccaac ggctgccttt gcggaccgcc tggaattcct tgcgacattt
     8161 cagcgttgca aggcgccctg ctgagatcgg tggctcagcc cctgcggcgg ccatttggca
     8221 cctgtcgccg gtccctgcgg ttaagcagaa agacgcttgc gtcccttggc ccgccgccgc
     8281 gccaccacac gccgcccacc cgttgtgagc atgcgcttgc gaaagccgtg ccggcgcttg
     8341 cgcacgagct tgctcggctg atacgttcgt ttcacctcta aactccgtcc gaaagccgcc
     8401 gtgcggcgag gtgatcgccg ctgcaagagc tcttcatcgc agatgagctg cgtgatgacc
     8461 actgccggcg tcgagcctgg cgaaagtttg cgcgcttata ggggcgccct tttgccaagt
     8521 caatccggcc caaggaggag ccgctggcct gccgaagcat caattttcgc cgcctcctct
     8581 cacgagtgct tgaatatggc cgcacctgga cttggcaact gtggggtgga ggtgccttct
     8641 actagactgc cgcaaccagg c
//
LOCUS       DASTMT010000601         2413 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_4489339, whole genome shotgun sequence.
ACCESSION   DASTMT010000601 DASTMT010000000
VERSION     DASTMT010000601.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2413)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2413)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2413
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4489339"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            187..2136
                     /gene="tkt"
                     /locus_tag="VFR00_13770"
     CDS             187..2136
                     /gene="tkt"
                     /locus_tag="VFR00_13770"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEU0160384.1"
                     /translation="MANAIRALAMDAVETAKSGHPGMPMGMADVATVLFSRFLKFDAA
                     DPAWPDRDRFVLSAGHGSMLLYALLHLTGFPGMSLAELRNFRQLAAKTAGHPEYGHAP
                     GIETTTGPLGQGLANAVGMALAERLLNQRFGDALVDHHTYVIVGDGCLMEGISQEAIS
                     LAGHLKLGKLIALFDDNAISIDGPTALAQSDRQLQRFEASGWGVQAIDGHDPTAIGAA
                     LAAAQGADRPQLIACKTTIGFGAPGKQGKASAHGEPLGAEEIKAARQALGWPYAAFEV
                     PEAVLAAWRLVGRRGAAARQAWEDRARGSARRLDADGLIGSQTLQTAIAASKSEFLAT
                     PPKIATRVASQKVLEQLVPALPALIGGSADLTTSNGTRTKHHKAITAQDFSGNYLHWG
                     VREHAMAAAMNGMALHGGIVPYGGTFLVFTDYCRPAIRLSALMGVRVIYVMTHDSIGL
                     GEDGPTHQPVEHLAALRAIPNLNVFRPADAVEVAECWELALAAATTPSVLALTRQAVP
                     ALRSDGGAENRSAKGAYVLAEAQGGARDVTLLATGSEVGLAIEAREALARDGIGAAVV
                     SMPCWELFAGQSVAYRAQVLGRAPRVAVEAAVAQGWERWLGEDGSFIGMSGFGASAPA
                     GKLFAHFGITASHVAAAARQLIKRP"
     gene            2133..>2413
                     /gene="pgk"
                     /locus_tag="VFR00_13775"
     CDS             2133..>2413
                     /gene="pgk"
                     /locus_tag="VFR00_13775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEU0160385.1"
                     /translation="MTFDELKSTAGLDLRGKRVLVRADLNVPIKDGKVSDATRLERLV
                     PGLAELAQRGARVIVISHFGRPRDGRDPHLSLAPVASKLGELLGRPVAFA"
BASE COUNT          369 a          846 c          836 g          362 t
ORIGIN      
        1 gggtggggaa aacctggccg ttggcagggc cgggttggac ctgcgccgat gcgccccctc
       61 gaggcttcta gacgcggcgc gcagcggcgg ctaatgctcg gccgcgatcg gcaaagcgac
      121 ctgcgaggac ccatggtgaa gccgggggca aactcaagcc cgccaacggg ggcggcccat
      181 gcggacatgg ccaacgccat tcgtgcgctg gcaatggatg ccgtcgaaac cgccaaatcg
      241 ggccacccgg gcatgcccat gggcatggcg gatgtggcaa ccgtgctgtt cagccgcttt
      301 ctgaagttcg atgccgccga tccggcctgg ccggaccggg accgcttcgt gctgtccgca
      361 gggcacggct ccatgctgct ctacgccctc ctgcacctca cgggctttcc gggcatgagc
      421 ctggccgagc tgcgcaactt ccgccagctt gcggccaaaa ccgccggcca ccccgagtat
      481 ggccatgccc ccggcatcga aacgaccacc gggccgctgg gccaggggct cgccaatgcc
      541 gtcggcatgg cgctcgccga gcgcctgctc aaccagcgct tcggggatgc cctcgtcgat
      601 caccacacct atgtgatcgt cggcgatggc tgcctgatgg aaggcatcag ccaggaggcg
      661 atctcgctcg ccggccacct taagctcggc aagctgatcg cgctgttcga cgacaatgcc
      721 atttccatcg acgggcccac cgcgctcgcc caatccgacc ggcaattgca gcgcttcgag
      781 gcttcgggct ggggcgtcca agccatcgac ggccacgacc cgaccgccat cggcgcggcc
      841 ttggcggccg cccagggggc cgaccggcca cagctgatcg cctgcaagac caccatcggt
      901 ttcggggcgc cgggcaagca ggggaaggcc tccgcccacg gcgaaccctt gggcgcggag
      961 gagattaagg ccgcgcgcca agccctgggc tggccctacg cggccttcga agtgcccgag
     1021 gcggtgttgg ccgcctggcg cttggtggga cgtcgcggcg ccgccgcccg gcaagcctgg
     1081 gaagaccgcg cccgcggcag cgcgcggcgg cttgatgcgg acggcctgat cggcagccaa
     1141 accctgcaga cggcgatcgc cgcgagcaag agcgagtttt tggcgacgcc ccccaaaatt
     1201 gccacgcgcg ttgcctccca gaaggtgctg gaacagctgg tgccggccct gccggcactg
     1261 atcggcggct cggcggacct gacgacctcg aacggcaccc gcaccaagca ccacaaggcc
     1321 atcacggcgc aggacttcag cggcaactat ctccactggg gcgtgcgcga gcacgccatg
     1381 gccgcggcca tgaacggcat ggcgctgcac ggcggcatcg tgccctacgg cggcaccttc
     1441 ctcgtcttta cggattattg ccgccccgcc atccgcctct cggcgctaat gggtgtgcgc
     1501 gtgatctatg tgatgacgca cgattccatc ggtcttggcg aagacggccc cacccaccaa
     1561 cccgtcgaac acctggccgc gctccgcgcc atcccgaacc tcaatgtgtt ccggcccgcc
     1621 gatgccgtcg aggtcgccga gtgctgggag ctggcgctgg cggccgccac gacgccgtcg
     1681 gtcctggcgc taacgcgcca ggcggtgccg gccttgcgca gcgacggcgg cgcggagaac
     1741 cgctcggcca agggcgccta cgtcttggcc gaggcgcaag gtggcgcccg tgacgtgacg
     1801 ctgctggcaa cgggttcgga ggtcggcctc gccatcgagg cgcgagaggc gctggccagg
     1861 gatgggatcg gcgcggcggt cgtgtcgatg ccgtgctggg aactgttcgc tgggcaaagt
     1921 gttgcctatc gcgcccaggt gctggggcgg gcgccgcggg ttgccgtcga ggcggctgtg
     1981 gcgcagggct gggagcgctg gctgggcgag gatggcagct tcatcggcat gtcgggcttc
     2041 ggcgcctcgg cgccggccgg caagctgttt gcacatttcg gcatcacggc cagccacgtc
     2101 gccgcagcgg cccgccaact gatcaagcgt ccatgacctt cgatgagctc aaatcgacgg
     2161 ccggtctcga cctccggggc aagcgcgtgc tcgtgcgcgc cgatctcaat gtgccgatca
     2221 aggacggcaa agtcagcgac gcaacgcggc tcgagcgact ggtgccgggc ctagctgagc
     2281 tggcgcagcg cggcgcgcgc gttatcgtca tctcccactt cgggcgtccg cgtgatggtc
     2341 gcgatcccca cctcagcctg gcgccggtgg cgagcaaact gggcgagctc ctcggccgac
     2401 cggtggcctt tgc
//
LOCUS       DASTMT010000602         8495 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_4491571, whole genome shotgun sequence.
ACCESSION   DASTMT010000602 DASTMT010000000
VERSION     DASTMT010000602.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 8495)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8495)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8495
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4491571"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..780
                     /locus_tag="VFR00_13780"
     CDS             <1..780
                     /locus_tag="VFR00_13780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0160386.1"
                     /translation="RNQAGVAFGPAGSKFANALAVTDNGANDLGHRRVANAPEKLFIV
                     TQAGQDAGFPDKEGFAFVTNKRFAWQAYRGNPVDRPLPQLYIGDKPFVPKMVPYRFQL
                     HVDGVRGVPLIAANPNPNGYINPVLEWDTNNPIDGIAWSARSFGADNNLFGAVYGILD
                     TGPESLVPTWPMVLRIEFLEPAGVKWSAFAQNIEPGPNAYQKPENRGGLERPNKVVFS
                     NDGRTMYVVDYGEVFTDYQMAPPMYTVPKSGVIWSITYEGR"
     gene            1192..1431
                     /locus_tag="VFR00_13785"
     CDS             1192..1431
                     /locus_tag="VFR00_13785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0160387.1"
                     /translation="MQVVYELAPVIAEIISGHCAGTRAREDFVQACLHGDWDEAKVMV
                     EGMLAEPWHLIGHQERRLREFLDLLHLRDGTLMSE"
     gene            1718..2890
                     /locus_tag="VFR00_13790"
     CDS             1718..2890
                     /locus_tag="VFR00_13790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015684740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0160388.1"
                     /translation="MTTTRWLAAPALLAAGALSAASAQAQELKIGIIAVLSGPQAVLG
                     GQLRDGFNLGVKHAGGKLGGLATSVVVQDDELKPDVAVGKVKQLIERDKVDFVVGPVF
                     SNILLAIHKPVTEAKVFLISPNAGPSPLAGKGCSSFFFATSYQNDQPPEVLGKYAQDK
                     GFKKAFLLAPNYQAGKDMVGGFKKHFKGEIADEVYTPLGQLDFSAELAKIASAKPDVF
                     FVFMPGGMGVNLVKQYRQAGLDNLPFLSVFTVDESTLPAQQDAAVGFFSGMTWAPNTD
                     TPENKRFVSDFEKEYGYVPGSYAMQAYDAAQLIGSAVKAVGGQLGDKEALRAALKQAE
                     FKSVRGPFKFANNGFPVQDFYLVRVAKRADGKFQTEIVEKVFANAVDSYAGDCALK"
     gene            3012..3938
                     /locus_tag="VFR00_13795"
     CDS             3012..3938
                     /locus_tag="VFR00_13795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009764219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEU0160389.1"
                     /translation="MSASLLVVQLFNGLQLGILLFLVAAGLTLVFGVMDFINLAHGVQ
                     YMLGAYLAVTAYRLTGNFFLAVLLALIASLLFGLLLELLVFRHLYARDHLEQVLATFG
                     VIMLLNQGVKVLWGAAPLNLPIPELLAGSVPLMEGLRYPVYRFAIIAAGVLVAAGLYL
                     FVTATRIGMLVRAGASNAPMVSALGIDIKRLFSLVFGFGAMLAGFAGIMIAPILSVEP
                     GMGDQILILAFVVIVIGGIGSIRGAFVAAVLVGVIDTLGRSFMTDLLRLVLAPATART
                     IGPALASMSIYLLMALVLAVRPAGLFPAKGRA"
     gene            3938..5194
                     /locus_tag="VFR00_13800"
     CDS             3938..5194
                     /locus_tag="VFR00_13800"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEU0160390.1"
                     /translation="MRQAMRAISQGASGVVAWPVSFDARMAVFYFALIAVLPLLAGRL
                     GGGYLLIIGERAVIFAIAALSLELLIGAGGLVSFGHAAFLGIGAYAGGIAASHGLGTL
                     SIALPAALAAAAAFALATGAIAVRTRGVYFIMITLAFAQMAFFIATSLAPYGGDDGLS
                     WPTRTTVFGARILKNETVFYYVGLALLILTYVCLGQLLGSRFGRVLRGLKDNDTRMQA
                     IGFTPYLYRLAAYVIAGTLCGVAGFLLGNQAEFVSPAYMHWQRSGELIIMVLLGGSGT
                     LYGPMLGALVFLLLEEALSHLTEHWKVVLGPALVLMALFARGGIAGAIAGWRQRRGAL
                     ARLASASARSARRHLAAGLARLPNGQRLGQHLRAYAAASRIDWLAARTRACWRVGKRA
                     LHRGLPGSTALSPRGLRTSIARWWQP"
     gene            5191..5940
                     /locus_tag="VFR00_13805"
     CDS             5191..5940
                     /locus_tag="VFR00_13805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008596566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0160391.1"
                     /translation="MTTTLLWLREVSKSFGALRVAEALELEVLAGETHAIIGPNGAGK
                     TTLINQISGALAPDAGHILFEGRDVTQLSMAARAHLGFARSFQITQVLPRFSALENVA
                     LAAQARAGSSFRFWRPAAAEAALNCTAMQSLAMVELAERAQAPAGTLSHGEKRRLEIA
                     IALAQAPKLLLLDEPMAGAGREESRALIGMLKGLKGRFGMLLVEHDMDAVFALADRVS
                     VLVAGRIIATGEPAAVRANPAVRAAYLGEAA"
     gene            5937..6644
                     /locus_tag="VFR00_13810"
     CDS             5937..6644
                     /locus_tag="VFR00_13810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018316881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0160392.1"
                     /translation="MSSALLSVAALEAGYGDQRVLFGFDLSVGTGEVASLLGRNGMGK
                     TTSVRAVMGLIPASAGRIEFAGRNLAGEPPFRIAQAGIGLVPEGRQIFPTLTVEENLV
                     ATAAHRHGRKWTLERVYAFLPRLAERRRHFGDQISGGEQQMLAIGRALLTNPRLLVLD
                     EATEGLAPLIRQEIWQGLAALKRDGLSLLIIDKNVDALLRLCDRHHIVEKGRVVWQGN
                     SAALAADGVLRDRYLSI"
     gene            6669..7115
                     /locus_tag="VFR00_13815"
     CDS             6669..7115
                     /locus_tag="VFR00_13815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012333418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEU0160393.1"
                     /translation="MKAMTAGVTKAGKGINNLSWNILGQTYIPKALSETSFSWHALLP
                     PGTFVPPHIHVNQDEFIYVLEGRFDLILDGKPGTASAGEFIRLPMQVMHGLFNKSDQP
                     IKCLFWVSPTGKLFELFKKIDRVPDPAEVTRLAALHDVNFLPPPPA"
     gene            complement(7223..>8495)
                     /locus_tag="VFR00_13820"
     CDS             complement(7223..>8495)
                     /locus_tag="VFR00_13820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012093333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flavin-dependent oxidoreductase"
                     /protein_id="HEU0160394.1"
                     /translation="GGLTLALMLKARGINCQIYETAPRLRELGVGINVLPHAIAELSE
                     LGLLARLDEVAIRTGELIYANRFGQEIWREPRGVEAGYEVPQFSIHRGHLQGVLRDAV
                     WQRLGRSVICADHTFASFAQEEGAVKARFRHATGARTTIAGDVLIGCDGIHSAVRRQL
                     YPRESGFKWNGVLMWRGATPAKAFLDGRTMIVGGGFNNKLVLYPIARGKGGKQLINWV
                     VTYRMGDGSNPPPRREDWNRRGMLAELLPHVARFQTPHVDLVKLVRGAPAFYEYPMAD
                     RDPLPRWTHGRVTLLGDAAHPMYPVGSNGASQAILDARCLADALVTAEHARAALTAYE
                     AERLPKTAEIVHNNRRGGPEGVIDEVERRAPDGFSDIDEVLSHRQRAAIVKGYAQLAG
                     FAKEQVNRRKSSRQRQPSSGRTAVMRKAKSR"
BASE COUNT         1335 a         2837 c         2717 g         1606 t
ORIGIN      
        1 cgcaaccaag ccggcgtcgc ttttggtccg gccggaagca agtttgcgaa cgccctggcc
       61 gtaaccgaca acggcgccaa cgatctcggc catcgacgcg tcgccaatgc gcccgagaag
      121 ctcttcatcg tgacgcaagc aggccaagac gcgggcttcc ctgacaagga aggcttcgcc
      181 ttcgtcacca acaagcgctt tgcgtggcaa gcctaccgcg gcaatccggt cgatcggccg
      241 ctgccgcagc tctacatcgg cgacaagcca ttcgtgccga agatggtgcc ctacaggttc
      301 caactgcacg tcgacggcgt acgtggcgtg ccgctgattg ccgcgaaccc gaacccgaac
      361 ggatacatca atccggtact cgaatgggac accaacaatc ccatcgatgg catcgcctgg
      421 agcgcgcgta gcttcggtgc cgacaataat ttgttcggcg ccgtttacgg cattctggac
      481 acgggtccgg aaagtctggt gccgacttgg cccatggtcc tgcgcatcga gttcctcgag
      541 cccgcgggcg tcaaatggtc agccttcgcg cagaacatcg agcccggtcc caatgcctat
      601 cagaagccgg aaaatcgcgg cggcctggag cgacccaaca aagtcgtatt ctctaatgat
      661 gggcgcacca tgtacgtggt cgactacggc gaggtcttca ccgactacca gatggcccct
      721 ccgatgtaca cggtaccgaa gtcgggcgtg atctggagca tcacctacga aggccgctga
      781 acgataccgg cgtcgccccg agggggcggc gccccaactc cccccttttc ggggcgtgat
      841 tgttttcttc ggggcgtgat tgtttttttt gcgagggtgc ctcgccccgt gcccggccgg
      901 ggcgcgctgc gatcaccgtt gcgcgctgag cgtggcaccg cggggcgcgg cgccgagttc
      961 gggctgacgc accaggcgcg cgcgccgcca aaatcgccac ccggcctcga cgctcgtgcc
     1021 ggagcggcgg ccggggccgc agccgcacgg cgcgggctgc cgtggtcgag gctgcaggcg
     1081 aactcgcgcg cgcaactcct tgagattcgg ggtggttttc gcctccacgg aacgtacagc
     1141 gccttggttg gttttacagc gggcccgcgc tgccgtttga acgaggttgc aatgcaagtc
     1201 gtttatgaac tggcacccgt gatcgccgaa atcatttccg gacattgcgc cggcacgcgg
     1261 gcgcgcgagg attttgtgca ggcctgcctg cacggggact gggatgaggc caaggtcatg
     1321 gtggaaggca tgctggccga gccgtggcac ctgatcggcc accaggagcg gcgcctgcgc
     1381 gagttcctcg acctcttgca cctgcgcgac ggcaccttga tgtccgaata gccgctcggt
     1441 cgcccggccg ttttgggcgg ccgtgtcggc gcaaacgcgc gtgagcgggc tcacccatgc
     1501 cgcgccgtcg cccgcccgcg ttgcgccttc gcgggggccc accgcagcag cccgtggcgc
     1561 cccaggcccc cgcctggcgc cgcctcctcg ccacgacatt gccggcaccg cagccgcgcc
     1621 gtaaagttcg cccgcgggaa aggtgcggcc ggcgggcgcc tcgccgccgc tccgcgaggg
     1681 aacagatggc gcgccgcgag gggacaagat gacgagaatg acgacgacaa ggtggctcgc
     1741 ggctcctgcc ctgctggcgg ccggcgcgct gtcggccgct tcggctcagg cccaagagct
     1801 caagatcggc atcattgcgg tgctgtcggg cccgcaggcc gtattgggcg gacaactgcg
     1861 cgatggcttc aatctcggcg tcaagcacgc gggcggcaag ctcggtgggt tggcgacgag
     1921 cgtcgtcgtg caggatgatg aactgaagcc cgacgtcgcc gtcggcaagg tcaagcaact
     1981 gatcgagcgc gacaaggtcg actttgtggt gggaccggta ttctccaaca tcctgctcgc
     2041 catccacaag cccgtcaccg aagccaaagt cttcctcatc agcccgaacg ccgggccttc
     2101 gccgcttgcc ggcaagggct gctcctcctt tttcttcgcc acctcctacc agaacgacca
     2161 gcccccggag gtgctgggca aatacgcgca ggacaagggt ttcaaaaagg cctttctgct
     2221 ggcgcccaat tatcaggccg gcaaggatat ggtcggcggc ttcaagaagc atttcaaagg
     2281 cgagatcgcc gatgaggtct acacacctct gggtcagctc gacttctccg ccgagcttgc
     2341 gaaaatcgcc agcgccaagc ccgacgtctt tttcgtgttt atgcccggcg gcatgggtgt
     2401 caacctcgtc aagcaatacc gccaggccgg cctcgacaac ctgccgtttc tttccgtctt
     2461 cacggtcgat gaatcgacgc tgccggcgca acaggatgcg gccgtcggct tcttctccgg
     2521 catgacttgg gcccccaaca ccgatacgcc cgagaacaaa cgcttcgtct ccgacttcga
     2581 gaaagagtac ggctacgtgc ctggcagtta tgccatgcag gcctatgacg cggcgcagtt
     2641 gatcggcagc gcggttaagg ccgtcggagg gcagcttggc gacaaggagg ccctgcgtgc
     2701 cgccctcaag caggcggagt tcaagtccgt gcgcggacct ttcaaattcg ccaacaacgg
     2761 ctttcccgtg caggatttct acctggtgcg ggtggccaag cgcgccgatg gcaagttcca
     2821 gaccgagatc gtagaaaagg tgtttgccaa tgccgtcgac agctacgccg gcgactgcgc
     2881 gctaaagtag ggcaacccgc aacggcaagg gcggccggtc gagcccaggg caagccgccc
     2941 agccgcaaac cctcgcggtc gaaaggttcg ctgccaccgc ccgctgcgtc gcgttgccaa
     3001 gggctttgcc aatgtctgcc tcgctgcttg tcgtgcaatt gttcaatggg ctgcagctcg
     3061 gcattctctt gttcctggtg gcggccggcc tgacgctcgt tttcggcgtc atggatttca
     3121 tcaatctggc gcacggcgtg cagtacatgc tgggtgccta cctggccgtg acagcctacc
     3181 ggctcaccgg caatttcttt ctggctgttc tgctcgccct catcgcctcg ctgctgttcg
     3241 gtttgctgct cgagctcctg gtgtttcgcc acctttacgc gcgcgaccat ctggaacagg
     3301 tgctggcgac gttcggcgtc atcatgcttc tcaatcaggg ggtgaaggtt ctgtgggggg
     3361 cggcccccct caacctgccg atccccgaac tgctggcggg atcggtgccc ctgatggagg
     3421 ggctgcgcta tcccgtctac cgcttcgcca tcatcgccgc gggagtgctg gtggcggcgg
     3481 ggctctatct cttcgtcacc gcgacgcgga tcggcatgtt ggtgcgcgcc ggcgccagca
     3541 atgcgccgat ggtttcggct ttggggatcg acatcaagcg gctctttagc ctggtgtttg
     3601 gattcggtgc catgctcgcc ggctttgccg gcatcatgat cgcaccaatc ctctccgttg
     3661 agccaggcat gggcgatcag atcctgatcc tggcattcgt cgtcatcgtc atcggcggca
     3721 ttggctcgat ccgcggcgcc tttgtggccg ccgtgctggt cggcgttatc gacacgcttg
     3781 ggcgctcgtt catgacggat ctcttgcgcc tcgtgctggc gccagccacg gcgcgcacga
     3841 ttgggcctgc gcttgcctcc atgtcgatct acctcttgat ggcgctcgtg ctcgccgtca
     3901 ggcctgccgg tctatttcct gccaagggac gagcctgatg cggcaggcca tgcgcgccat
     3961 ttcgcaaggc gcatcgggcg tcgtcgcgtg gcccgtgagc ttcgatgcac gcatggcggt
     4021 tttctatttt gcgctcatcg ccgtgctgcc actgctggct gggcgcctcg gcggcggcta
     4081 tctgcttatt attggcgaac gggcggtgat attcgccatc gccgcactgt ccctggaatt
     4141 gctgatcggg gcgggtggac tggtgtcctt cgggcacgcc gcctttctgg gcattggggc
     4201 ctacgcgggc gggatcgccg ccagccacgg gcttggcacc ttgtcgattg cgctgccggc
     4261 cgccctcgcc gccgcggccg ctttcgcgct cgcaaccggt gccatcgccg tgcgtacgcg
     4321 cggggtctac tttattatga tcacgctggc attcgcccaa atggcgtttt tcattgccac
     4381 ctcgctggcc ccctacgggg gtgatgatgg cctcagctgg ccgacgcgga caacggtgtt
     4441 cggggcgcgc atcctgaaga acgagaccgt cttctattat gtgggcctgg cgttgctgat
     4501 cctcacctat gtttgcctcg gtcagctgtt ggggtcccgc tttggtcgcg tgctgcgtgg
     4561 cctcaaggac aatgacacac gcatgcaggc gatcggcttc acgccctacc tctaccggct
     4621 tgccgcctac gtcatcgccg gcacgctctg cggcgtcgcc ggctttttgc tcggcaacca
     4681 ggcggagttc gtcagccccg cctacatgca ttggcagcgt tctggtgaat tgatcatcat
     4741 ggtcctgctg gggggcagcg gcacgctcta tggtcccatg ctcggcgccc tggtcttcct
     4801 gctgctggag gaagccctgt cgcacctgac cgagcactgg aaggtcgtgc tcggacccgc
     4861 tctcgtgctg atggcgctgt tcgcccgcgg cggcatcgcg ggcgccatcg ccggttggcg
     4921 gcaacggcgc ggcgcgctgg cccggttggc gagcgcgagc gctcggtccg cccgtcgtca
     4981 cctcgccgcc ggcctggcgc gtctgccgaa cgggcagcgc ctcggacaac atctgcgcgc
     5041 gtacgccgcc gcgtcccgca ttgactggct ggccgccaga acgcgcgcct gctggcgagt
     5101 gggcaaacgg gcgctgcacc gcggcctgcc cggttcgacc gccttgagcc cacgcgggct
     5161 cagaaccagc attgcccgct ggtggcagcc atgaccacga cgctgttgtg gctgcgcgag
     5221 gtcagcaaat cgttcggcgc cttgcgcgtc gccgaggcgc tggagctcga ggtcctggcc
     5281 ggtgaaacgc acgcgatcat cggtcccaac ggcgccggca agaccaccct catcaaccag
     5341 atttccggcg cgcttgcccc cgatgcgggc cacatcctgt tcgagggtcg cgatgtcacg
     5401 caattgtcga tggcggcccg cgcgcatctg ggcttcgccc gctccttcca gattacccaa
     5461 gtgctgccgc gcttttcggc gctcgaaaac gtggcgcttg ccgcgcaggc acgggccggc
     5521 tcgagcttcc gcttctggcg ccctgccgcc gccgaggcgg ccctcaactg cacggccatg
     5581 caatccctcg ccatggtcga gctggcagag cgcgcgcagg ctcccgccgg caccctgtcc
     5641 cacggcgaga agcgccggct agagattgcc atcgcgctgg cgcaagcgcc caagctgctc
     5701 ctgctcgacg aaccgatggc gggggctggc cgcgaggaaa gccgcgctct gatcggcatg
     5761 ctcaagggcc tcaagggtcg cttcggcatg cttctggtcg agcacgacat ggacgccgtg
     5821 ttcgcgctgg ccgaccgggt ctccgtgctg gtcgccggcc ggatcatcgc caccggcgag
     5881 cctgccgcgg tgcgtgccaa tccggccgta cgggccgcct accttggcga ggccgcatga
     5941 gctcggcact gctcagcgtt gccgcgctcg aggccggcta cggcgaccag cgcgttctct
     6001 tcgggttcga tctgtcggtc ggcaccggcg aggtggcaag cctgctcgga cgtaacggca
     6061 tgggcaagac caccagcgtg cgggccgtga tgggcttgat accagccagc gcgggccgca
     6121 tcgaattcgc cggccgcaac cttgccggcg agccgccgtt tcgtattgct caggcgggga
     6181 tcggcctagt gccggaaggg cggcaaatct ttcccacgct gacggtcgag gaaaacctgg
     6241 tggccacggc ggcgcatcgg catgggcgca agtggacgct cgagcgcgtc tacgcctttt
     6301 tgcctcggct cgccgagcgc cggcgccact tcggcgacca gatttccggc ggcgagcagc
     6361 aaatgctggc gatcggccgg gccctgctga ccaatccgcg cttgctggtg ctcgatgagg
     6421 ccaccgaggg cttggcgccg ttgatccgcc aggagatttg gcaggggctt gccgccctca
     6481 agcgcgacgg cctctccctc ctcatcattg ataagaatgt cgacgccctg ttgcgtctgt
     6541 gcgaccgtca tcacattgtg gagaagggac gggtcgtgtg gcagggcaat tcggcggcgc
     6601 tcgccgccga tggggttctg cgcgaccgct acctttcgat ctgaattcgc agggcaggag
     6661 tacgtgcgat gaaagcaatg acggcaggcg tcaccaaggc gggtaagggt atcaacaatt
     6721 tgtcgtggaa catccttggc cagacctata tccccaaggc gctgtccgaa acgagcttct
     6781 cctggcacgc cttgctgccg cccggcacct tcgtgccgcc tcacattcac gtcaaccaag
     6841 acgagttcat ctatgtcttg gaaggccgtt ttgacctcat tctcgacggc aagcccggca
     6901 ccgcctcggc cggcgaattc attcgcctgc cgatgcaggt catgcatggc ctcttcaaca
     6961 aaagcgacca gcccatcaag tgcctgttct gggtctcgcc caccggcaaa ctgttcgagc
     7021 tttttaagaa gatcgatcgc gtgcccgatc cggcagaggt cacgcgcctg gccgccctgc
     7081 acgacgtcaa cttcctgccg cccccgcccg cctagttggc gctggatgag cgccgttcgc
     7141 ctcgcagatg tcggacgcac acctgctgtc ggtcgcgacc gtggtctgtt gcccactgcc
     7201 ggcgtgcaag gtcatgcgga ctttagcgag atttcgcttt ccgcatcacg gcggtgcgtc
     7261 cgcttgacgg ctggcgctgg cgcgaggatt tgcgccggtt gacctgctcc ttggcaaagc
     7321 cggccagctg agcgtaaccc ttgacgattg ccgcacgctg ccggtggctc agaacctcat
     7381 cgatgtcgct gaaaccatcg ggcgcgcgcc gctccacctc atcgatcacg ccttccggcc
     7441 cacctcggcg attgttgtgt acgatctcgg ccgtcttcgg caagcgctcg gcctcatagg
     7501 cggtcagcgc cgcccgagca tgttcggccg taacgagcgc atcggccaga cagcgcgcgt
     7561 cgaggatcgc ctggctggcg ccgttggagc cgacgggata catgggatgg gcggcatcgc
     7621 ccaagagcgt cacgcggcca tgcgtccagc gtggcagggg atcgcggtct gccattgggt
     7681 actcgtagaa tgcgggcgca ccgcgcacga gcttcaccaa atcgacgtgc ggcgtctgaa
     7741 agcgagcaac gtgcggcagt agctccgcca gcatgccccg tcggttccaa tcctcccggc
     7801 gcggcggcgg gttcgagccg tcgcccatgc gataggtcac gacccaatta atcaactgct
     7861 taccgccttt gccgcgcgca atgggataga gcaccagctt gttgttgaag ccgccaccga
     7921 cgatcatggt gcgcccgtcg aggaaggctt tggcgggcgt ggccccgcgc cacatcaaga
     7981 cgccattcca cttgaagccg ctttcgcgcg gatagagctg ccgtcgcacg gccgaatgaa
     8041 tgccgtcgca gccaatcaga acatcgccgg caatcgtggt gcgtgcgcca gtcgcgtgcc
     8101 tgaagcgcgc tttgacggcg ccttcctcct gggcgaagga ggcaaaggtg tgatcagcgc
     8161 agatcaccga tcgaccgagc cgctgccaca ccgcatcgcg cagaacgcct tgcagatgtc
     8221 cgcggtgaat ggagaactgc ggcacctcgt agccggcctc cactccgcgc ggctcgcgcc
     8281 atatctcctg accgaaacgg ttggcgtaaa tcaactcgcc cgtcctgatt gccacttcgt
     8341 cgagccgagc cagcaggccg agctcgctca gctcggcaat cgcgtgcggc agcacgttaa
     8401 tgccgacccc gagctcgcga agtctgggcg ccgtctcgta gatctggcag ttgatgcctc
     8461 gcgccttgag catgagggcg agggtcagcc caccg
//
LOCUS       DASTMT010000603         6122 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_5475191, whole genome shotgun sequence.
ACCESSION   DASTMT010000603 DASTMT010000000
VERSION     DASTMT010000603.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 6122)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6122)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6122
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5475191"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1537
                     /locus_tag="VFR00_13825"
     CDS             <1..1537
                     /locus_tag="VFR00_13825"
                     /inference="COORDINATES: protein motif:HMM:NF024584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HEU0160395.1"
                     /translation="AVRPDPPGSGVLPLDRPQALPAPRRAYWQAEPDARIVWESASRF
                     SAQRPAREEGGRFLASALIPVPAVRSLSLELTLAGTAVDLAVRVLDCGGAVIASETLA
                     LAAAMAPRILRLAVPTEATSAVAIELCHGPQAFAVSVDLGRTRRPAPIADPCPAVPEP
                     ARWEATTVGAVIRWSQRRQTIRWDGAASLYLAKSYSRPCLPGVTLRYPVRLCVRAGAL
                     GIGVLSEDFQSWVKTFRFETGEHATTLEIEVGSNARVQIVVYACERGRLDAEIDWTEA
                     MQQHPISALELDRLAARARALAVERNGADPAGPSAPDKLARRAGRVAAKAMLAAAPAA
                     GDATAKRPEPASRPPRGGSVWRLIHDPRTVHCHKPWTDLHNFTVDGRMDVCCIATGAS
                     QTRFALGNLSEQSFQEVWNGGAARKFRRTVNDPVQALPPCQRCPMAQSYAGPLFNPEH
                     TALSVWRRVRRLPVPLAQTRPGRFLLLIGFLLLYGPVHLWMFRGFRRATLLPPFKTLW
                     QWQ"
     gene            1584..3578
                     /gene="asnB"
                     /locus_tag="VFR00_13830"
     CDS             1584..3578
                     /gene="asnB"
                     /locus_tag="VFR00_13830"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HEU0160396.1"
                     /translation="MCGLAGLITLNERAQIDRGLLDGMAATLAHRGPDGRGVWLEAQR
                     RIGLAHRRLAIIDLDAKAAQPMADAGGCIRVTYNGEIYNHAALRRRLEEVGARFLTDH
                     SDTEVLLQGYQAWGIDGLLARLEGMFAFALYDGNLDALFLVRDRMGIKPLYFTEHNGL
                     FAFASEIKALLALPDLPRRVDAAAAYHYLSFMVAPAPASLFAGIAKLPAGHLLEIGRD
                     RRPKARRWWSAADHIAVAAEKSPKPGTNSEAIGEVRRLLQASVEKHMVADIPVGVFLS
                     GGVDSSTALALMAQVSSRPVRSFTVGFRDEESLNELAEARAAADLYGAEHHEVMIGEA
                     DAEACLASLIQHQDEALADWVCIPLYFVAKLAASTGTKAILVGEGADELFFGYDNYLK
                     FLRLHERIARLGRFAPAIAGPILRLARDLLPRERLGLIGLFDHLYRGLAQREVFWTGA
                     IAFWEGQKRRITAPAGAGDAQGWSAYGLREIGSYERDSFAIVAALKRALASRVPHSAQ
                     VDRMRYAEFSLRLPELLLMRTDKMTMAHSLEARVPFLDHTLVEYALGLDRTALLPAGQ
                     TKGLLKAAVADLLPAEVLARPKRGFGAPMARWLRGAFGARRLEQLEASRALAAIGLDR
                     AAVLGLFRAHRSGRRDLSLQLWPIINFALWYDHWIEGHSL"
     gene            3602..5212
                     /locus_tag="VFR00_13835"
     CDS             3602..5212
                     /locus_tag="VFR00_13835"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEU0160397.1"
                     /translation="MVRRLLLLTRPRHPGEDFAFAETLSLSELKALVRSGQVANRILR
                     YNEVVLRTEHLHAFPRPFLLACLLRTLSRGACIVEDHSGCQARIGWPQICRQGMAYLR
                     DWWAAPRARAAVERQLGELEARNRPAARPIPGRGRALYLRSDLWLGTNVGGALAHTAG
                     IANALADAGMAPVLLAYTPLPTLRAAVEQRQLPIPPRYWNLPELPAIAANLQLAPLLA
                     AAIAELQPAFLYHRYAQMSYAGALAARRLGIPLILEYNGSEVWIANTWGAGLRYEGLA
                     RRIEDAVLRAADRIVTVSEPLSAELRARGLPAERIVTQPNGVDTRRFRPDLDGAPLRR
                     RLGIAGHEIVIGFIGSFAVWHGISVLVAAYARLIGAHSQLRQTTRLLLIGDGALRGAI
                     EAEIEALGLVDRTILAGAIPQGEAPHYLAATDILAAPHVPNPDGSEFFGSPTKLFEYM
                     AMGRCIVASAIGQIGDILSHERTALLVRPGESAPLAAALLRAVSNPALRQALGAAARR
                     QAVERHDWRQALANILAPPASSLGKARQ"
     gene            5301..>6122
                     /gene="wecB"
                     /locus_tag="VFR00_13840"
     CDS             5301..>6122
                     /gene="wecB"
                     /locus_tag="VFR00_13840"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HEU0160398.1"
                     /translation="MIKALLVIGTRPEAIKMAGLVYELRRRRGIHWKLASTGQHRSML
                     DETLCDLGIAPDVDLGIMQRVSGLAGITAAVMHSLAPVICDYKPDWVFVQGDTTTAMT
                     AALAAVYHKTQVGHVEAGLRTGNRYSPWPEEINRKVIGTIATRHYAPTPTARENLLKE
                     GVACDDVLVTGNTVIDALLYMSTRTRDDAEFKRQLADEFPWLGGNRRMILVTGHRRES
                     FGDGFRQICLALLRLAERDDVEIVYPVHLNPNVRKPVQTMLSASARIHLIEPQRYS"
BASE COUNT          943 a         2135 c         1973 g         1071 t
ORIGIN      
        1 ggccgtgcgg cccgatcccc cgggatcggg cgtactgccg ctcgatcgcc cgcaggcgct
       61 accagcgccg cgccgcgcct attggcaagc cgagccggat gcccgcatcg tctgggaaag
      121 cgcgagccgc ttcagcgccc agcgcccagc acgcgaggag ggcggtcggt tcctggcctc
      181 ggcactgatc ccggttccgg cggtgcggtc gctgagcctg gagctgacgc tcgccggcac
      241 ggccgtcgat cttgccgtgc gtgtccttga ttgtggaggc gccgtgatcg cctctgagac
      301 cttggcgctc gcggccgcga tggccccgcg catcctccgg ctcgccgtgc caaccgaggc
      361 aacctccgcc gtcgccatcg agctctgcca cgggccgcaa gccttcgcgg tttcggtcga
      421 tctcggccga acgcggcgcc cggccccgat cgccgatccc tgtcccgccg tgccggaacc
      481 ggcgcgctgg gaagccacca ccgtcggcgc cgtgatccgt tggtcccagc gccggcaaac
      541 gatccgctgg gacggcgcag catcgctcta tctggccaag agctattcgc gtccctgtct
      601 gcccggtgtc accttacgct atcccgtgcg gctctgcgtg cgcgccggcg cgctgggcat
      661 cggcgtcttg tccgaggact tccagtcctg ggtgaagacc tttcgcttcg agaccggcga
      721 gcacgcaacg acgctcgaga tcgaggtggg cagcaacgcg cgcgtgcaaa tcgtcgtcta
      781 tgcctgcgag cgcgggcgcc tcgatgccga aatcgattgg accgaggcca tgcagcagca
      841 cccgatctcg gccctcgagc tggaccggct cgcggcgcgg gcgcgcgcgc ttgcggtaga
      901 acggaatggc gcggaccccg cggggccgag tgcccccgac aagctcgcgc gccgggcggg
      961 ccgcgtggcg gccaaggcga tgctcgcggc ggccccggct gcgggcgacg caacggccaa
     1021 gcggcctgag ccggcgtcgc ggccgccccg gggcggctcc gtctggcgtc tgatccacga
     1081 tccgcgcacg gtccactgcc acaagccctg gacggatctg cacaatttca ccgtcgacgg
     1141 ccgcatggat gtctgctgca tcgccaccgg agccagccag acgcgtttcg ccctcggcaa
     1201 cctgagcgag cagagcttcc aggaggtctg gaatggcggc gccgcccgga aattccggcg
     1261 cacggtcaac gatcccgtgc aagccctgcc gccttgccag cgctgcccga tggcacaaag
     1321 ctacgccggt ccgctgttca acccggagca tacagccttg agcgtgtggc gccgcgttcg
     1381 acgcttacct gtgcccctcg cccagaccag gcccggacgc tttctgttgc ttattggttt
     1441 tttactgctg tacggaccgg ttcacctctg gatgttccgc ggcttccgcc gcgcgacgct
     1501 gttgccgccc ttcaagacgc tttggcaatg gcaatgacgg cacgacgtgg caggccctgg
     1561 cgcggcggca ggcgagcgcg aacgtgtgcg ggctagccgg gctcatcacg ctcaacgagc
     1621 gcgcgcagat cgatcggggt ttactcgacg gcatggccgc gaccctggcg catcgtggtc
     1681 cggatggtcg gggcgtgtgg ctggaggcac agcggcgcat cggtcttgcc caccgccgcc
     1741 tcgctatcat cgacctcgac gccaaagcgg cgcagccaat ggccgacgcc ggcgggtgca
     1801 ttcgcgtcac ctacaatggc gagatctaca atcacgccgc gctgcggcgc cgattggaag
     1861 aggtcggcgc ccgcttcctg accgaccact ccgacaccga ggtcctgctg cagggttacc
     1921 aagcttgggg catcgacggc ctgctggcgc gcctcgaagg catgttcgcc ttcgcccttt
     1981 atgacggcaa tctcgacgct ctctttctcg tgcgcgaccg catgggcatc aagccgcttt
     2041 acttcaccga gcataatggc ctttttgcct ttgcctccga gatcaaggcg ctgttggcgc
     2101 tgcccgacct gccgcggcgg gtggatgcgg cggccgcgta ccattacctg tcgttcatgg
     2161 tggcgccggc gccggcttcc ctgttcgcgg ggatcgccaa gttgcctgcc gggcatttgc
     2221 tcgagatcgg gcgcgatcgc cgaccgaagg cccggcgctg gtggagcgcg gccgaccaca
     2281 ttgccgtcgc ggcggaaaaa tcaccaaagc ccggaaccaa cagcgaggcc ataggcgagg
     2341 tgcgccgact gctgcaggcc tcggtcgaga agcacatggt cgccgatatt cccgttggcg
     2401 tcttcctctc gggcggcgtc gacagctcga cggcactcgc gctgatggcg caggtctcca
     2461 gcaggccggt gcgaagcttc accgtcggtt tccgcgacga agaaagcctc aacgagctcg
     2521 ccgaagcgcg cgccgccgcc gacctctacg gggccgagca tcacgaggtc atgatcggcg
     2581 aggccgacgc ggaagcgtgc cttgccagcc tgatccagca ccaggacgag gcgctggccg
     2641 actgggtttg cattcccctt tacttcgttg ccaagctggc ggccagtacc ggcaccaagg
     2701 ccatcctggt cggcgaaggc gccgacgagt tgttcttcgg ctacgacaat tacctgaaat
     2761 tcctgcgcct gcacgagcgc attgcgcgcc tcggccgctt cgcaccggcg atagcgggac
     2821 ccatcctgcg tttggctcgg gatcttttgc cgcgcgagcg cctgggcctg atcggtcttt
     2881 tcgaccacct ctatcgtgga ctggcgcagc gtgaggtgtt ctggacgggc gccatcgcct
     2941 tttgggaagg ccagaaacga cgcataacgg cgccggctgg cgccggcgat gcgcagggat
     3001 ggtcggctta cggtttacgt gagatcggct cttacgagcg cgactccttt gccatcgtcg
     3061 ccgcgctgaa gcgggcgctt gcgagccgtg ttccccacag cgctcaggtc gaccgcatgc
     3121 gctacgccga attcagcttg cgcttgcccg agctccttct catgcgcacc gacaagatga
     3181 cgatggcgca ttcgctcgaa gcgcgcgtgc ccttcctcga ccatacgctc gtcgaatatg
     3241 cgcttgggct cgatcgcacc gcgctgctcc ccgccggtca aaccaagggc ctgctcaagg
     3301 ccgccgtcgc cgacctgctg ccggcagagg tgttggcgcg gcccaagcgt ggcttcgggg
     3361 cgccgatggc gcgttggctc aggggtgctt ttggcgcccg ccgactggaa cagctcgagg
     3421 cctcccgcgc gctcgccgcc atcggcttgg atcgcgccgc cgtgctgggg ctgttccgcg
     3481 cgcatcgcag cggccggcgc gatctttccc tgcagctttg gccgattatc aatttcgcgc
     3541 tctggtacga tcattggatc gaaggccaca gcctctaatg caaaaggatc agtaaagcac
     3601 gatggtaaga cgccttctcc tgctgacgcg gccgcggcac ccaggtgaag attttgcctt
     3661 cgccgaaacg ctcagccttt ccgagttaaa agcgctcgtg cgctccggac aggtcgccaa
     3721 ccgaattctg cgctacaacg aggtcgtttt acgtaccgaa catttgcacg cctttccccg
     3781 tccctttctg ctggcctgct tgcttcggac cctgtcccgc ggggcgtgta tcgtcgagga
     3841 tcactctggc tgccaagcac gcatcggttg gccgcagatt tgccggcaag gcatggccta
     3901 cctgcgcgat tggtgggcgg ctccccgggc acgggccgcg gtcgaacggc agctcggcga
     3961 actcgaggcg cgcaaccggc ctgcggcgcg ccccatcccg gggcggggcc gtgcgctcta
     4021 tttgcgcagc gacctgtggc tcggcaccaa cgtcggcggc gcgctcgccc atacggcggg
     4081 tattgccaac gccctcgctg atgcgggcat ggcccccgtg ctgttggcct acacgccgct
     4141 gccgaccctg cgcgctgccg tcgagcagcg ccagctgcca atcccacccc gctattggaa
     4201 cttgccggaa ctgccggcca tcgccgccaa tttgcagctc gcgccgctgc tcgcggccgc
     4261 gatcgccgag ttgcaaccgg cgttcctcta ccaccgctat gcgcaaatga gttacgccgg
     4321 cgccctggca gcgcgccggc tcggcatacc gctcatcctc gaatacaacg gctcggaggt
     4381 ttggatcgcc aacacatggg gggcggggct gcgctatgag gggctcgcgc ggcgcatcga
     4441 ggacgcggtc ctgagggccg cggaccggat cgtcaccgtc tcggagccac tctcggccga
     4501 actcagggcg cgcggccttc ccgccgagcg tatcgtcacc caacccaacg gcgtcgatac
     4561 ccgacgtttt cgccccgatc tcgacggcgc gccgctgcgc cggcgcctcg gcatcgccgg
     4621 gcacgaaatc gtgatcggct tcatcggcag cttcgccgtt tggcatggca tttccgtctt
     4681 ggtcgccgcc tatgcacgcc tcatcggcgc gcactcccag ctgcgacaaa cgacgcgcct
     4741 gctgctgatc ggcgacggcg ccctgcgggg cgcgatcgag gctgagatcg aggcgctggg
     4801 cctcgtcgat cgcacaatct tggccggcgc cattccccag ggcgaagcgc cccactattt
     4861 ggccgcaacc gatatcctgg ccgcaccgca cgtgccgaat cccgacggct cggaattctt
     4921 tggctcgccc accaagctct tcgaatacat ggccatgggg cgctgtatcg tcgcctccgc
     4981 catcggccag atcggggata ttctcagcca tgagcgaacg gcattgctcg tgcgcccggg
     5041 cgaatcggcg ccgctagccg ccgcactgtt gcgtgccgtg tccaacccgg cgctgcgcca
     5101 ggcgctgggc gcggccgcgc gtcgccaggc ggtcgagcgg catgactggc gccaggcgct
     5161 ggcgaacatc ctcgcacccc ctgcatcatc cctggggaaa gcgcgacaat gagcattgtg
     5221 atcactgtcg ggtgcggtat ctaacggcat ggaagagtag gtcttttggg ggcaccgccc
     5281 ttccttgagg gggcattctt ttgatcaaag cgcttttggt gatcgggacc cgcccggaag
     5341 ccatcaaaat ggcgggactg gtctacgaac tgcgacgacg gcgcggcatc cactggaaac
     5401 tcgcctcgac gggccagcac cgctcgatgt tggacgagac gctttgtgac ctcggtatcg
     5461 cccccgatgt cgatctcggc atcatgcagc gggtgagcgg gctggccggc atcacggccg
     5521 cggtcatgca cagccttgct ccggtgatct gcgactacaa gccggactgg gtctttgtgc
     5581 aaggcgacac gacgacggcc atgacggccg cgcttgccgc cgtctatcac aagacccagg
     5641 tcggccacgt cgaggcaggc cttagaacgg ggaatcgcta cagcccgtgg cccgaagaga
     5701 tcaatcgcaa ggtcattggc accatcgcca cccgccacta tgcgccgacg ccgacggcgc
     5761 gcgaaaactt gttgaaggag ggcgtcgcct gcgacgacgt cctcgtcacc ggcaacaccg
     5821 tgatcgacgc acttctgtac atgagcacac gcacgcgcga cgatgcggag ttcaagcgcc
     5881 agctcgccga cgaattccct tggcttggcg gcaaccggcg catgatcctg gtcaccgggc
     5941 atcggcggga gagcttcggc gacggctttc ggcaaatctg tctcgccttg ctccggctcg
     6001 ccgagcgcga cgacgtcgag atcgtctatc ccgtgcatct caaccccaat gtacgcaaac
     6061 ccgtgcagac gatgctgagt gcgagcgcgc gcatccacct gatagagccg caacgctatt
     6121 cc
//
LOCUS       DASTMT010000604         7521 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_5475354, whole genome shotgun sequence.
ACCESSION   DASTMT010000604 DASTMT010000000
VERSION     DASTMT010000604.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 7521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7521
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5475354"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..937)
                     /gene="aspS"
                     /locus_tag="VFR00_13845"
     CDS             complement(<1..937)
                     /gene="aspS"
                     /locus_tag="VFR00_13845"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013949710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEU0160399.1"
                     /translation="MSESKMSDSAPGHGHRYRSHTCGALRGGDGGHSVRLSGWVHRVR
                     DHGGLLFIDLRDHYGITQVVVDPDSPAFAMAESVRSEWVICVDGRVRQRPEGTVNAEL
                     PTGAVEVFAGQIEVLSAAKELPVPVFGEPDYPEDLRLQYRFLDLRRDQLHRNIMLRNE
                     IIASIRRRMREGGFFEFQTPILTASSPEGARDYLVPSRVHPGKFYALPQAPQQFKQLI
                     MIAGFDRYFQIAPCFRDEDARADRSPGEFYQLDLEMSFVEQEDVFAAVEPVMRGLFEE
                     FGGGRPVTQTVPRIPYDEAIQKYGSDKPDLRNPIVM"
     gene            1270..2493
                     /gene="rnd"
                     /locus_tag="VFR00_13850"
     CDS             1270..2493
                     /gene="rnd"
                     /locus_tag="VFR00_13850"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013502101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HEU0160400.1"
                     /translation="MRPIVTTSELAQACARLRRHPFVAVDTEFIREHTFWPKLCLIQM
                     GGPEEELIVDPLADGLDLTPFYQLMADERVTKVFHAARQDLEIVWTQAQLIPHPIFDT
                     QVAAMVCGFGEAVSYSNLVKQVTGRDLDKTSRFTDWARRPLSQRQLTYALGDVTHLRD
                     IYRYLRAELDKEGRAGWLDEEMAELTDPKTYQTSPEEAWRRLKLRVKNRRGLAVLIEL
                     AAWRERAAQAQDVPRNRILRDEALFDIASHAPASPAELAELRTLSEGFARSARAKEIL
                     EAVKRGLARDVTTLPALRAGQPLAADKVALLELLRVLLKGCAAREKVAPRLIADGDDL
                     ERIAIEEHPDVAAMKGWRYEMFGRQAELLKRGALALTVAAGEVVTVQLPAAPQLTEAA
                     AGKESGALERKRGAG"
     gene            complement(2490..3095)
                     /locus_tag="VFR00_13855"
     CDS             complement(2490..3095)
                     /locus_tag="VFR00_13855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019400364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEU0160401.1"
                     /translation="MKLLSSLLSPYVRKVRIAAAMKGLSDGIELVPIDTNVPDNPAIN
                     RANPLAKVPALLVDGVAIYDSNVICEYLDGLAAAPVLFPKDAAARIKTLTLGALGDGI
                     LDAALLLVYEKRYRPEEKWHAPWQQRQQDKIDRALDLLEQSPPTWGQSPDYGHLTLAC
                     ALGYLDFRHEGKWRPAHPKLVAWVDAFARAVPAFEATRPQA"
     gene            complement(3234..4436)
                     /locus_tag="VFR00_13860"
     CDS             complement(3234..4436)
                     /locus_tag="VFR00_13860"
                     /inference="COORDINATES: protein motif:HMM:NF025597.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4339 domain-containing protein"
                     /protein_id="HEU0160402.1"
                     /translation="MTAMPAPSSQNQWYLARDGQQYGPLSDPELAKFVELGHLQPTDL
                     LWREGFPDWRPALVVFPQGGAAARKEGPAPATPNLNVRPAGAPGDDAARPSANRPNGA
                     AATLADGDDDLEGRRRGFGRRLLKLLFIGALVLAAAWFAWTWRSDLSKLIGAIGASSP
                     TSVLAATDRAGIEVPPLRGFGPDTAQMDANLQATPLWRILKREFPDWYADRLKEVAAA
                     IQQQKDDAAIAQQMGRALVDLRRKQVENALSASLPSLKRIASTFYDNIAELRKQSTDA
                     CFAFISGGEANPMIVALLQGSPHTARLQSQLVSVFEAVADGRKLPRVYPKPRKTDYET
                     LATALTGLGWSQADMQLFSDERELARAAPDKICQLVHDWFAAQLGLKDADAQLRLLVD
                     SLRPVVAG"
     gene            4693..5115
                     /gene="ndk"
                     /locus_tag="VFR00_13865"
     CDS             4693..5115
                     /gene="ndk"
                     /locus_tag="VFR00_13865"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEU0160403.1"
                     /translation="MAIERTLSIIKPDATARNQTGAVNALIEKAGLRIIAQKRVRWSQ
                     QQAEKFYEVHKARPFYADLVRFMTSGPIVVQVLEGDGAIAKYRDVMGATDPAKAAAGT
                     IRKELAESIERNSVHGSDSAENAKLEIGLNFKPEEIVG"
     gene            5173..5673
                     /locus_tag="VFR00_13870"
     CDS             5173..5673
                     /locus_tag="VFR00_13870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018264235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEU0160404.1"
                     /translation="MLTPAYVRTMAAYNRWQNQNLFAAADALPEAERKAPRRAFFGSI
                     HGTLNHLVWADQLWMRRFAGEVRPAAVGIAQSPDLYAHWDDLKRERVALDDAILAWAH
                     ALEATWLEGNLSWYSNSAKRELTRPKGLIVAHFFNHQTHHRGQVHCLMTQCGVIPADT
                     DLPIMP"
     gene            5748..>7521
                     /locus_tag="VFR00_13875"
     CDS             5748..>7521
                     /locus_tag="VFR00_13875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012334094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEU0160405.1"
                     /translation="MNAPARIGIGHSVCPHDCPSTCALDVELLGDNRIGRVHGSADHS
                     YTAGVVCAKVARYAERVHHPDRLMHPLRRKGGKGSGEFEKIGWDAALDLVAEALLAAE
                     RRHGSEAVWPYYYAGTMGLLMRDGINRLRHAKRYSGMYATICTTLAWTGYIAGTGRLA
                     GPDPREMAKSDLVVIWGTNPVNTQVNVMTHAVRARKERGAKIVAVDIYHNGTMQQADL
                     ALCLRPGTDGALACAVMHVLFRDGYANWPYLEKYSDCPRELEAHLKSRTPEWASAITG
                     LSVAEIETFARMVGTTPRSYFRLGYGFARSRNGAHNMHAALCIPTVTGAWLHEGGGGF
                     HNNGAIYHWNKSLIEGLDVVDPSIRVLDQSRIGPVLTGDPYDIKGGPPVTALFVQNMN
                     PVQVAPEQRKVKAGFARSDLFVCVHEQFMTATAKAADVVLPATMFLEHDDIYQGGGHQ
                     HIMLGPKLLEPPGECRSNHDVIAALAKRLGAEHPGFAMSPRQIIDWTLQRSGWGTLAE
                     LEPNKWIDCQPNFETAHYLGGFRYPDGKFRFKPDWQSVPAPRANAWIVERGMPPLPDH
                     WDVVEKADARHPFRLATSPAREYLN"
BASE COUNT         1278 a         2480 c         2528 g         1235 t
ORIGIN      
        1 gcataacgat ggggttgcgc aggtccggct tgtcggagcc gtacttctgg atcgcctcgt
       61 cgtaggggat gcgcgggacc gtctgcgtca ccggccggcc gccgccgaat tcctcgaaca
      121 ggccgcgcat cacgggctcg acggccgcga acacgtcctc ctgctcgacg aagctcatct
      181 ccaggtcgag ctggtagaac tcccccggcg agcggtcggc gcgcgcgtcc tcgtcgcgaa
      241 agcacggggc gatctggaag tagcggtcga agccggcgat cataatgagc tgtttgaatt
      301 gctggggcgc ctgcggcagg gcgtagaatt tgccggggtg cacgcgcgag ggcaccaagt
      361 agtcccgcgc gccctcgggg ctcgacgccg tcagaatcgg ggtttgaaac tcgaaaaacc
      421 cgccctcgcg catgcggcgc cggatcgagg cgatgatctc gttgcgcagc atgatgtttc
      481 ggtgcagctg atcgcgccta agatcgagga agcggtattg cagccgcaag tcctcggggt
      541 agtccggctc gccgaacacg ggaaccggca gctccttggc agccgacaac acctcgattt
      601 gccccgcaaa cacctccacg gcaccggtcg gcagctcggc attgacggtg ccctcgggcc
      661 gctggcgcac gcggccatcg acgcagatca cccactcgga gcgcacgctc tcggccatgg
      721 cgaaggcggg gctatccgga tccaccacca cctgggtgat gccgtaatgg tcgcgcaggt
      781 cgatgaacag gagcccgccg tgatcgcgca cgcggtggac ccagcccgac aggcgcacgc
      841 tgtggccgcc gtcgccccca cgcagcgccc cgcaggtgtg gctgcggtag cggtgcccgt
      901 gcccgggcgc cgaatcgctc attttgctct cggacatggg acttagagcc tcatgcaaat
      961 agcttcggga tcgctagagt gcggaaaagc acatgggggt gacgcaaagt caagattggg
     1021 ccgggcggcc ctggccaggg ccgacagcca agtgcgccaa gccgctgcgg gtgaaggttg
     1081 gcgccttggc cttggggctt acgccgccgt tggggtcgcg gccggccggc caagggggta
     1141 tgcggcgtac cgctgccggc cgttggggtc gcccgccctc gccgacgcgc cctcgcgggc
     1201 gccaagggtt agccaggagc atgcgccggt cgcgacaacc gatatggctt gggctataag
     1261 gcggcgctta tgcgcccgat cgtcacaacc tccgagcttg cccaagcctg cgcacgcctc
     1321 aggcggcatc ctttcgtggc tgttgatacg gaattcatcc gcgagcacac cttctggccc
     1381 aagctgtgcc tcattcagat gggggggccc gaggaggagc tgatcgtcga cccgctcgcc
     1441 gacggcctcg atctcacccc cttctatcag ttgatggcgg acgagcgcgt caccaaggtc
     1501 tttcacgcgg cccgacagga tctcgagatc gtctggaccc aggcccagct gatcccgcac
     1561 cccattttcg atacgcaagt cgccgccatg gtgtgcggct tcggcgaggc cgtgagctac
     1621 agcaacctcg tcaagcaggt caccggcagg gatctcgaca agacctcgcg cttcaccgac
     1681 tgggcgcggc ggccattgtc ccagcgtcag ctgacctatg cgctgggtga tgtcacgcac
     1741 ctgcgcgaca tctatcgcta cctgcgcgcg gagctggaca aggagggcag ggcggggtgg
     1801 ctcgatgagg agatggccga gctgaccgat ccgaaaacct accaaacctc gccggaggag
     1861 gcctggcgcc ggctcaagct gcgcgtcaag aaccgccgcg gtttggccgt gctgatcgag
     1921 cttgccgcct ggcgcgaacg cgccgcgcag gcccaggacg tgccgcgcaa ccgcattctg
     1981 cgcgacgagg cgctgttcga catcgccagc cacgccccgg cgagcccggc cgaactggcg
     2041 gaactgcgca cgctcagcga gggcttcgcc cgctcggcgc gcgccaagga gatcctggaa
     2101 gccgtcaagc gcggtctggc gcgcgacgtc accacgttgc cggcgctgcg cgcgggccag
     2161 ccgctggccg ccgacaaggt cgccttgctc gaactcctgc gcgtgctgct caagggctgc
     2221 gcggcacgcg aaaaggtggc cccacgtctg atcgcggatg gcgacgacct cgagcgcatt
     2281 gccatcgagg agcatcccga cgtcgccgcc atgaagggct ggcgctacga aatgttcggc
     2341 agacaggccg agcttttgaa gcgcggggcg ttggccttaa cggttgcggc cggcgaggtc
     2401 gtgaccgtgc agctgccggc ggccccgcaa ctgaccgagg ccgccgcggg caaggagagc
     2461 ggcgcgctcg agcgcaagcg cggtgccggc taggcctgcg gccgcgtcgc ctcgaaggcg
     2521 ggcaccgccc gcgcgaaggc gtcgacccag gccaccagct ttggatgcgc ggggcgccat
     2581 ttgccctcgt gacgaaagtc gagatagccc agcgcgcagg caagcgtcag gtgtccgtag
     2641 tcggggcttt gcccccaggt gggcgggctt tgttcgagga gatcgagggc gcgatcgatc
     2701 ttgtcctgct ggcgctgctg ccagggcgcg tgccactttt cctcgggccg atagcgcttt
     2761 tcatagacga gcagcagggc cgcatcgagg atgccgtcgc cgagggcgcc aagggtgagc
     2821 gtcttgatgc gcgccgcggc atccttcggg aacagcacgg gggcagcggc cagcccgtcg
     2881 aggtattcgc agatcacgtt gctgtcgtag atggcgacgc catcgaccag caatgccggc
     2941 accttggcca gcggattggc gcgattgatc gcgggattgt cgggaacgtt ggtgtcgatc
     3001 ggaaccaatt caattccgtc gctcagcccc ttcatggcgg cggcaattcg caccttgcga
     3061 acgtaaggtg acagcaggct cgacagcagc ttcatgtgcg gcgccctttg ttgcgaactg
     3121 cggccaatgg tgccccggtc cgggccgacc agcaatgaga tttcttggcg ccccgggctg
     3181 cgccgcgcgg ctcgggcggt gccacgtcca ctgcgggggg tgggcagagg cggtcagccc
     3241 gcgaccaccg gccgtaacga atcgaccagc aggcgcagct gcgcgtcggc atccttgagg
     3301 ccgagttggg cggcgaacca gtcgtgcacc agctggcaga tcttgtcggg cgccgcgcgc
     3361 gccagctcgc gttcatcgga gaagagctgc atgtcggcct ggctccaacc gagcccggtg
     3421 agcgcggtgg cgagcgtctc atagtcggtc ttgcgcggct tggggtagac gcgcggcagt
     3481 ttgcgcccgt cggcgacggc ttcgaatacc gacaccagct gggactgcag acgcgccgta
     3541 tgcggcgagc cttgcagcag cgccacgatc atgggattgg cctcaccccc ggagatgaag
     3601 gcaaaacagg catccgtgct ctgtttgcgc agctcggcga tgttgtcata aaacgtgctg
     3661 gcgatgcgct tgaggctcgg caggctggcc gagagcgcgt tctcgacctg cttgcggcgc
     3721 agatccacca aggcgcgccc catttgctgg gcaatggcgg catcgtcctt ctgctgttgg
     3781 attgccgccg ccacctcctt caggcgatcg gcataccagt cgggaaattc gcgcttgagg
     3841 atgcgccaca acggtgtcgc ctgcaggttg gcgtccatct gtgccgtgtc tgggccaaag
     3901 cctcgcagcg gcggcacctc gatgccggcg cgatcggtgg cggccagcac cgaagtgggt
     3961 gagctcgcgc cgatggcgcc gatcagcttg ctgagatcgc ttcgccaggt ccaagcgaac
     4021 caggcggcgg ccaagacgag ggcgccgatg aaaagaagtt tgaggagccg ccgaccaaag
     4081 ccgcgacgcc tgccttcgag gtcgtcgtcg ccgtccgcga gcgttgcggc tgccccgttg
     4141 gggcggttgg cgctgggacg tgccgcgtca tcgccgggcg cgcccgccgg tctcacgttc
     4201 agattgggcg ttgcgggggc cgggccctct ttgcgtgccg ccgcgccgcc ttgcggaaac
     4261 accaccagcg ccgggcgcca gtcgggaaag ccctcgcgcc agagcaagtc cgtcggctgc
     4321 aggtgcccga gctccacgaa cttggcgagc tccgggtccg agagcggtcc gtactgctgg
     4381 ccatcgcggg ccagatacca ctgattttgc gaggacggcg cgggcatagc ggtcattcga
     4441 gcatcggctt gccgtcggtg acggtaggct tcccattttt tccaatttca gggcaagagt
     4501 tccccggcgt caatcgccga agccccaagg ggttagcagc gaagcaaggc cgagggaagg
     4561 cagcggcaag accgagcctg agacaccaat cgccgcgttc gcggccaaaa ttgggcgccc
     4621 ggcttgaacc ccgcctcggc cgcaagctat aacccgcgcc atcatagatg aaaaacccgc
     4681 caggagtcat ggatggcgat tgagcgcacc ctgtcgatta tcaagcccga cgccaccgcc
     4741 cgcaaccaga ccggcgccgt caacgccctg atcgaaaagg cgggcctgcg gatcattgcg
     4801 caaaagcggg tgcgctggag ccagcagcag gcggaaaaat tctacgaggt gcacaaggcg
     4861 cggccgtttt acgccgacct cgtccgcttc atgacctccg gaccgatcgt ggtccaggtg
     4921 ctcgagggcg atggcgccat tgcgaagtac cgggacgtga tgggagccac cgatccggcc
     4981 aaggccgccg ccggcaccat tcgcaaggag ttggccgaaa gcatcgagcg caattccgtg
     5041 cacggctccg acagcgccga gaatgccaag ctcgagattg ggctcaactt caagcccgag
     5101 gagatcgtcg gctaagacag tgcgggcgtg ctggcgcgca cgcgcgccca cgtttttctt
     5161 cggggggatg ccatgctcac gcccgcctac gtgcgcacca tggcagccta taatcgctgg
     5221 cagaaccaaa acctctttgc cgctgcggat gcgctgcccg aggcggaacg caaggcgccg
     5281 cggcgggcct tcttcgggtc gatccacggc accctcaatc atctcgtgtg ggcggatcaa
     5341 ctctggatgc gccgctttgc cggcgaggtg cggcccgccg cggttgggat tgcgcaatcg
     5401 cccgatctct atgcgcactg ggacgatctg aagcgtgagc gcgttgccct cgacgacgcc
     5461 atcctggcat gggcgcatgc cctcgaggca acctggttag agggcaacct cagctggtat
     5521 tcgaactcgg ccaagcgtga gctgacgcgg cctaaggggc tgatcgtggc gcacttcttc
     5581 aatcatcaga cgcaccatcg cgggcaggtg cactgtctca tgacgcaatg cggcgtgatc
     5641 cccgccgata ccgatctgcc gatcatgcct tgaggcgggc gcggcatttc ttggccaatg
     5701 ccgcgaactc ggttagcgtg gcggcagtgt tgctgcgaag gacgcccatg aacgcccccg
     5761 cccgcattgg gattggccac tcggtctgtc cgcacgattg cccttcgacc tgtgcgctgg
     5821 atgtcgagtt gctgggggac aaccgcatcg gccgcgtgca cggctcggcc gatcacagct
     5881 acacggccgg cgtcgtgtgc gccaaggtgg cccgctacgc cgagcgcgtg catcatcccg
     5941 atcggctgat gcatccgctc cgccgcaagg gcggcaaggg gtcgggcgaa ttcgagaaga
     6001 tcggctggga tgcggcgctc gacctcgtcg ccgaagcgct gctggcggcc gagcgccggc
     6061 atggctcgga ggccgtgtgg ccctactatt atgccgggac catgggcctc ctcatgcgcg
     6121 atggcatcaa ccggttgcgc catgccaagc gctactcggg catgtacgcc accatctgca
     6181 cgaccttggc ctggaccggc tatatcgccg gcaccgggcg gctggccggc cccgatccgc
     6241 gcgagatggc caaatccgac ctggtggtga tttgggggac caatccggtc aacacgcagg
     6301 tcaacgtgat gacgcacgcc gtgcgcgcgc gcaaggagcg cggcgccaag atcgtggcgg
     6361 tcgacattta tcacaacggc accatgcagc aggccgatct tgccctctgt ctgcgtccgg
     6421 gcacggacgg tgcgctcgcc tgcgcggtga tgcacgtctt gtttcgcgat ggctatgcca
     6481 attggcccta cctcgagaag tacagcgatt gcccgcgtga gctggaggcg cacctaaaat
     6541 cgaggacgcc ggagtgggca agcgccatca ccgggctcag cgtggccgag atcgagacct
     6601 tcgcgcgcat ggtcggcacc acgcccagga gctactttcg cctcggctat ggctttgccc
     6661 gctcgcgcaa cggcgcccac aacatgcacg cggccttgtg catcccgacc gtgacggggg
     6721 cctggctcca cgagggcggc ggcggtttcc acaacaacgg cgccatctat cattggaaca
     6781 aatccttgat cgagggcctc gacgtggtcg acccgagcat tcgcgtgctc gatcagtccc
     6841 gcatcggtcc ggtcctgacc ggtgatcctt atgacatcaa gggcgggccg ccggttaccg
     6901 cgctgtttgt gcagaacatg aacccggtgc aggtcgcgcc cgagcagcgc aaggtcaagg
     6961 ccggcttcgc ccgcagcgat ctcttcgtgt gcgtgcacga gcagttcatg acggcgaccg
     7021 ccaaagcggc cgacgtggtg ttgccggcca ccatgttcct ggagcacgac gacatctatc
     7081 aaggcggcgg ccaccagcac atcatgctcg gccccaagct gctcgagccc ccgggcgaat
     7141 gccgctccaa ccacgatgtc atcgccgccc tggcgaaacg gctgggcgcc gagcatccgg
     7201 gctttgccat gtcgccacgc cagattatcg actggacgct gcagcgctcg ggctggggca
     7261 cgctggccga gctcgagccc aacaagtgga tcgactgtca gccgaacttc gaaaccgccc
     7321 actacctcgg gggttttcgc tatcccgacg gcaaattccg cttcaaaccc gattggcaaa
     7381 gcgtgccagc gccacgcgcg aacgcctgga tcgtcgaacg gggcatgccg ccgctgcccg
     7441 atcactggga tgtggtggag aaggccgacg cccggcatcc tttccggctt gccaccagcc
     7501 cagcgcgcga atacctcaac t
//
LOCUS       DASTMT010000605         5344 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_5771091, whole genome shotgun sequence.
ACCESSION   DASTMT010000605 DASTMT010000000
VERSION     DASTMT010000605.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 5344)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5344)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5344
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5771091"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1801
                     /locus_tag="VFR00_13880"
     CDS             <1..1801
                     /locus_tag="VFR00_13880"
                     /inference="COORDINATES: protein motif:HMM:NF014071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HEU0160406.1"
                     /translation="LITTAGFKYVLEIGRQEVPRRASLFAWVKPKRPVPPELIFEVGG
                     RLAPDGTELSPLDEAAALAAARALKAEGIDAIAVVLLHSYANGSHERRVGEILRLEMP
                     EALISLSSEVLPVFREYERSMTTILNATVMPVVSTYVAELDRRVRDEGIAAPLLLMKS
                     SGGVTSTRTVRRAPVETALSGPAAGAVGAAYIGASAGHPNLIGIDIGGTSADISVIQA
                     SEPGLTTNGRIGGWPVGLPMVDLVTIGAGGGSIARLAGAGTLVVGPESAGAQPGPVSY
                     GRGGREPTVTDAHLVLGHLPPYLLGGRFALDVEAARAAIASKIADPLGLSVEAAARGI
                     LAIVDNNMVGAIRVVSVERGHDPRDFSLLPFGGAGPLHGGALARLLGIPKIVVPPAPG
                     VLSALGLLVSSLKAEFTRTCLQKAGAFDTIEVARVFQALDHEARAWLDQEEVPATARR
                     ILWHASLRYQHQGFELTVPWTGREVTETTAAATVAAFHRLHERLYTFAQEDTPVEFVT
                     LRVDAQGVFPTPNLNELPAATDLAEARRGTRRVFLESGAKEATVYAREHLGAGARIPG
                     PAILTQLDATTLILEGQVGLVDRFGNVVISETA"
     gene            1914..2948
                     /locus_tag="VFR00_13885"
     CDS             1914..2948
                     /locus_tag="VFR00_13885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008974114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEU0160407.1"
                     /translation="MLKVVAMAAIALGLISPAQAQTSRLASEDLSVPFGNGQIFVRNK
                     RPEGVASFASDRIVLFVHGATYPGTAFDLPLGGKSWMDYVAERGFDTYALDLPSYGRS
                     TRAPAMEVAAEDNPPLTRGDEAVKAVGAVVDHVLKRRGTGKLILIGWSWGTTIVATYA
                     TERANTVERVVLYAPVWLRTTPSLVQVQGKLGAYRTVSRDQALARWLTGVPEDKKATL
                     IPAGWFDTWADVTFATDPKGGGKTLRAPNGVVQDGLEFWGATPPKAYWDPEKLLAPTL
                     LVLGEWDKDTPPYMAQTLFPLLTKATWKRLAILSEGTHTMAMEKNRVLLFRTVQQFLE
                     EAPPTEAASQ"
     gene            complement(2954..>5344)
                     /locus_tag="VFR00_13890"
     CDS             complement(2954..>5344)
                     /locus_tag="VFR00_13890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0160408.1"
                     /translation="LVDDCTIWFAESELPARHSPYAIARSIARSWLGLTDHNTAQDVE
                     RRLSAILQQFADTLSDHAPALKALLNLSVADTDWPNYDLIDRKRRLAACFLEISRLQA
                     RHRPLFIIVDDAQWCDPETLQVLAEFTHHIKAMSAGLILLERAGNDTPLLESFPSAST
                     IRLRELSAEETREFLGVVMGSDASLEPVKAKLTEMAGGLPFFLEEAVRYLTRNGILTG
                     DAGNYTVVASAGALNIPDTVEALVSSRISSLPTDLRSVLSAASIVGRTGPLALIARIC
                     DSSVADVQPKLENLERQHIIAISELKSETIFEFRHEFFRQVAYAMTLSSDRRVLHAKT
                     LRAAEVLFDNRISDWITFLAYHAALASLHADAARYCRIAAEHAVLASSYSAASEFCEQ
                     AVKHLDQLPQTVENVQASIDVRLLLRVAAGGTSDFQSWLHHLGKSIELAEQIGDMSRR
                     LLALIHRTSAINFSSSAVDAVRAGEAAVGLAQALRIPSSEALARYALGQAHYVNGAYR
                     DAIESLTPATQWLAQGHEMEQLGTTGTTLVLCRMMQANSHASMGYFQAADGELARLED
                     LASQTGRSYDQVALSYCRGMVWGGKGQLDPTVNAFEHAYELCRRHGINLFIPVIAAGL
                     SAVLVASRNIARASEVALFGLEVAERLGHNVARTAATAALAGVRIAEGNPREAIRLAS
                     EARSAASAYAHRGVEVVATRVLAQAFLASNPGDLERPLFLLREAVELGESIEAIPSVS
                     SCGVLLVGTLNRSGQREEAIRSCKHLIALTADRDMPAQTGRLRGLLAELTGDAALA"
BASE COUNT          921 a         1751 c         1808 g          864 t
ORIGIN      
        1 gctcatcacg actgccggct tcaagtacgt gctggagatc ggacgccagg aggtgccgcg
       61 ccgcgccagc ctgttcgctt gggtcaaacc aaagcgtccg gtgccaccgg aactgatctt
      121 cgaagtcggc gggcggctcg cgcccgacgg taccgaactc tccccgctcg acgaggccgc
      181 cgcgcttgcg gccgcgcgcg cgctcaaagc cgagggcatc gacgccatcg ccgtggtgct
      241 gctgcacagt tacgccaatg gcagccacga gcgccgcgtc ggcgaaatcc tacggctcga
      301 aatgcccgag gcgctcatct cgctgtcgag cgaggtgctg cccgtgttca gggaatacga
      361 gcgcagcatg accaccattc tgaacgcgac ggtcatgccg gtggtgtcga cctatgtggc
      421 cgagctcgat cggcgcgtca gggatgaggg tatcgccgcg ccgctcctgc tcatgaaatc
      481 gagcggcggc gtcaccagca cacgcaccgt gcgccgtgcg cccgtggaga ccgcactctc
      541 cggccccgcg gctggcgccg tcggcgcggc ctacatcggt gccagcgccg gccatcccaa
      601 cctcatcggt atcgatatcg gcggcacctc ggccgacatt tctgtgatcc aagcgagcga
      661 gcccgggctc acaaccaatg gccgcattgg cggctggccg gtcggcctcc ccatggtcga
      721 cctggtgacg atcggcgcgg gtggcggctc gatcgcgcgc ctcgccggcg cgggcacgct
      781 ggtggtcgga cccgaaagcg ccggcgcaca acccgggccg gtctcctatg gccggggcgg
      841 ccgcgagccg acggtcacgg acgcgcactt ggtcctgggg catctgccgc cctacctgct
      901 cgggggtcgc tttgccctcg acgttgaggc ggcgcgcgcc gccatcgcca gcaaaatcgc
      961 cgatccccta gggctcagcg tcgaggcggc ggcgcgcggg atcctggcca tcgtcgacaa
     1021 caacatggtg ggggccatcc gcgtcgtgtc ggtagaacgc ggccacgacc cgcgtgactt
     1081 ttcgctactg ccctttggcg gcgccgggcc gctgcacggc ggcgcgctcg cgcgtttgct
     1141 cgggatcccc aaaatcgtcg tgccaccggc gccgggcgtg ctctcggcgc tggggctgtt
     1201 ggtgtcgagc ctcaaggccg agttcacgcg cacgtgcctg caaaaagccg gcgccttcga
     1261 caccatcgag gtggcgcgcg tattccaggc gctcgatcac gaggcccggg cctggctcga
     1321 tcaggaggag gtgcccgcca ccgcgcgccg cattctctgg cacgccagcc tgcgctacca
     1381 gcatcagggt ttcgagctga ccgtcccttg gacaggacgc gaggtgaccg agaccaccgc
     1441 ggcggcgacc gtcgccgcct tccaccggtt gcatgagcgc ctctacacct ttgcccagga
     1501 ggacacgccc gtcgagttcg tcaccttgag ggttgacgcc cagggcgtgt ttcccacgcc
     1561 aaacctgaac gagctaccag ccgcaaccga cctggctgag gcgcggcggg gcacccggag
     1621 ggtatttctg gaaagcgggg caaaagaggc cacggtttac gcccgcgagc acctcggcgc
     1681 cggggcgcgc atccccgggc ccgccatcct cacccagctc gatgccacca cccttattct
     1741 cgaggggcag gtgggcctcg tcgatcgctt cgggaatgtg gtcatcagcg aaactgcctg
     1801 atccgaggct ctggcctggt ccgaacagac gacattgccg agctcgggca ttgacgaggg
     1861 caagcgcgcg ggccgaggat gggtcagaaa agctcatttt cgggaggtaa tcgatgctca
     1921 aggtggtggc gatggcggcg atcgcgctcg gcctgatatc ccccgcacag gcgcaaacct
     1981 cgcgccttgc cagcgaggat ctctcggtcc cgttcggcaa cggtcagatc ttcgtgcgca
     2041 acaagcgtcc ggagggagtg gcgagctttg cgagcgatcg catcgtgctc ttcgtgcacg
     2101 gcgcgaccta cccgggcacc gccttcgatc tgccgctggg cggcaagtcc tggatggact
     2161 atgtcgccga gcgcggcttc gacacctatg ccctcgactt gccgagttat gggcggtcga
     2221 cgcgcgcacc ggccatggag gtcgccgccg aggacaatcc accgctgacg cgaggtgacg
     2281 aagccgtcaa ggccgtgggc gccgtcgtcg atcacgtctt gaagcggcgc ggcacgggaa
     2341 agctgatcct gatcggctgg tcctggggca ccacgatcgt tgccacttat gccaccgagc
     2401 gcgccaacac ggtcgaacgc gtcgtgctct atgcaccggt ctggctgcgc accacaccgt
     2461 ccctggtgca agtgcagggc aagctcggcg cctatcgtac cgtgagccgc gaccaggccc
     2521 tggcgcgctg gctgaccggg gtccccgagg acaagaaggc gacgttgatc cccgccggtt
     2581 ggttcgacac ctgggccgac gtgacgtttg ccaccgatcc caagggcggc ggcaagacgc
     2641 tgagggcgcc taacggtgtg gtccaagacg gtctggaatt ctggggcgcg acgccgccca
     2701 aagcctactg ggatccggaa aaactgctgg ccccaacgct cctggtgctc ggcgaatggg
     2761 acaaggatac cccgccctac atggcccaga cgctctttcc cctgctcacc aaggcgacct
     2821 ggaagcggct cgccatcctg agcgagggga cgcacaccat ggcaatggag aagaaccggg
     2881 tgctgttgtt ccgcaccgtg cagcagttcc tcgaggaagc gccgccgacc gaggccgcca
     2941 gccagtaaag gtcttaagcc agggccgcat ccccggttag ttcagccaac aggccgcgca
     3001 ggcgcccagt ttgcgcgggc atgtcccgat cggccgtcag ggcgatcaga tgcttgcagg
     3061 atctgatcgc ctcctcgcgc tggccgctcc ggttcaaggt gcccaccagc aacacgccac
     3121 agcttgaaac ggacgggatc gcctcgatcg actcgccgag ctcgaccgct tcgcgcaaca
     3181 gaaacaacgg tcgctccaga tccccggggt tgctcgccag gaaggcttgg gccagcacac
     3241 gcgtggcaac cacctcgacg ccacgatggg cataggcgct ggccgccgag cgtgcctcgc
     3301 tcgcaagccg aatggcctcg cgcggattgc cttcggcgat gcgaacgccg gccagggccg
     3361 ccgtggcggc cgtgcgcgcg acgttgtggc caagcctctc cgccacttcc aggccgaaga
     3421 gggccacctc gctcgcacgc gcaatgttgc gcgacgcgac caggacggcg ctcagacccg
     3481 ccgcaatgac ggggatgaag aggttgatcc cgtgccggcg gcaaagctcg taggcatgct
     3541 caaaggcgtt gacggtggga tcgagctgac ctttgccgcc ccaaaccatg ccgcgacagt
     3601 aggaaagcgc gacttgatcg taggagcgcc cggtttggct cgccagatcc tctaggcggg
     3661 cgagttcgcc gtcggccgcc tggaagtacc ccatcgaggc atgcgagttg gcttgcatca
     3721 tgcggcacag gacgagcgtc gttcccgttg tgcccaattg ctccatctca tggccctggg
     3781 ccagccattg cgtggcgggc gtcaagcttt cgatcgcatc ccggtaggcg ccgttgacgt
     3841 agtgggcctg ccccagggca tagcgggcca gcgcctcgct cgaggggatg cgcaaggcct
     3901 gcgccagccc gaccgcggcc tccccggcgc gcacggcgtc gaccgccgag ctcgagaaat
     3961 tgatggccga ggtgcggtga atgagggcga gcaggcggcg ggacatgtcc ccgatctgct
     4021 cggcgagctc gatcgacttt cccaggtggt gcagccagct ctgaaaatcc gaggtgccgc
     4081 cggcggccac gcgcagcaag agacggacgt cgatcgaggc ctggacgttt tcgaccgttt
     4141 gcggcagctg atccaagtgc ttgacggcct gctcgcaaaa ctccgatgcc gcactgtagg
     4201 aggaggcgag gacggcgtgt tcggcggcga tgcggcagta acgggcggcg tcggcgtgca
     4261 ggctcgctag cgccgcgtga taggcgagga acgtgatcca atcggagatg cggttgtcga
     4321 acagcacctc ggccgcgcgc aaggtcttgg cgtgcaggac ccgccggtcg ctgctcaagg
     4381 tcatggcata ggcgacctga cggaaaaact cgtggcgaaa ctcgaagatc gtttcggact
     4441 tgagttccga gatggcgatg atgtgttggc gctcgagatt ttccaatttc ggctgcacgt
     4501 ccgcgaccga gctgtcgcaa atgcgcgcga tcaaggcgag cggcccggtg cggccgacaa
     4561 tagaggccgc ggacagcacg gagcgaaggt cggttggcaa gctcgagata cgggaggaga
     4621 cgagagcctc caccgtgtcc gggatattga gagcaccggc cgaggccact accgtgtagt
     4681 tgccggcgtc gccggtcaga atgccgttgc gggtgagata gcgaaccgct tcctccagga
     4741 agaagggcaa tccacccgcc atttccgtca gcttggcttt gacgggctcg agagaggcgt
     4801 ccgaccccat cacgacgccc aaaaactcgc gtgtttcctc cgcactcagc tcccgcagcc
     4861 gaatggtgct ggcacttgga aagctctcca gcaggggggt gtcattcccg gcccgttcga
     4921 gcaggatgag cccggcggac atggccttga tatgatgggt aaattcggcc aggacctgca
     4981 gggtctccgg gtcacaccat tgcgcgtcat cgacaataat aaagaggggc cggtgccgcg
     5041 cctgtaggcg cgaaatttcc agaaaacagg cggccaatcg gcgtttgcgg tcgatgagat
     5101 cgtagtttgg ccagtccgta tcggcgacgc tgagattgag gagcgccttg agggcggggg
     5161 cgtggtcgga cagcgtatcg gcgaactgct gcaggatggc cgagagccgg cgctccacat
     5221 cctgtgccgt gttgtgatcg gtcagtccga gccagctgcg ggcgatggag cgggcgatgg
     5281 catagggcga gtggcgcgcc ggcagctcgc tttcggcaaa ccagatggtg cagtcatcga
     5341 ccag
//
LOCUS       DASTMT010000606         8622 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_5475723, whole genome shotgun sequence.
ACCESSION   DASTMT010000606 DASTMT010000000
VERSION     DASTMT010000606.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 8622)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8622)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8622
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5475723"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(132..872)
                     /locus_tag="VFR00_13895"
     CDS             complement(132..872)
                     /locus_tag="VFR00_13895"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEU0160409.1"
                     /translation="MRRYDFTASRLYVETDLAPGKLVTCRPDQVNYLRHVLRLNAGDR
                     ILVFNGRDGEWQASIGEARKGAMTLHAESQVRPQQGAPDIDYLFAPLKRARLDYMVQK
                     ATEMGVARLRPVFTRRTTPERINGARMRANAIEAAEQCGILRVPEVLEPETLGAALAH
                     WQVERHLVFCDEDSEAADPLAALRSLAPGPIALLIGPEGGFDAGERELITSQPFALSI
                     SLGPRILRADTAAVAALALVNATLGDWR"
     gene            complement(928..1905)
                     /gene="argC"
                     /locus_tag="VFR00_13900"
     CDS             complement(928..1905)
                     /gene="argC"
                     /locus_tag="VFR00_13900"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019402973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HEU0160410.1"
                     /translation="MPTKIASEPRAALATAKRPAVFIDGEAGTTGLQIRRMLEAVPSL
                     EVKSVAPAERKEPRARLAIMREVDLVVLCLPDAAAKEAVALMASLPGGGPRIVDGSTA
                     HRVAEGWVYGFPELSKEQAAAVRQARRVSNPGCYPTGAIALVRPLVEAGLLPCDYPLT
                     VNAVSGYSGGGRSMIEGYEAGDAPVFELYGLNLEHKHVPELQTYAKLTRRPLFVPSVG
                     NFRQGMLVSVPLHLDTLPGEPSAADLEAALARYYAGTSVKVLPTVEPAEQPPRLEPEG
                     LNGSNGLELRVFANAKYRQAVLVARLDNLGKGAAGQALQNIGLMLGVPV"
     gene            complement(2082..3347)
                     /locus_tag="VFR00_13905"
     CDS             complement(2082..3347)
                     /locus_tag="VFR00_13905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011083316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0160411.1"
                     /translation="MTSNKAHAGTRPSAPGEERRTLAAAGSAHILHDGYTDLLYLLLP
                     IWQAEFGLGYAEVGLLRSSYTGVMAALQIPAALLAERCGPVLLLVAGTLVSALAFLLA
                     GASLGLVSLLLVLVLGGIGASVQHPIGSSLVSAAFSGARSRTALGVYNFTGDLGKMAM
                     PLLAALLLSTMPWRSALWVLGGLGIACAVLVLALAPAQANASRRDDEAVAPLRAAASA
                     AEVPSRQGFLLLLAIAMIDSAGRSGFLTFLPFLLKAKEASIASIGSALSLLFVGGAAG
                     KLVCGYLGARVGLFNTVLLTEGLTAIGIIALIPLPMAGAFMLMPLLGIGLNGTSSALY
                     GTVPELVAPARRTRAFGIFYTGAIAASAAMPPLIGLIGDGFGVATAMGLIAVAVLSTV
                     PLAALLVPMLPTSRRSALEARGDGGGCGH"
     gene            complement(3352..4086)
                     /gene="trmD"
                     /locus_tag="VFR00_13910"
     CDS             complement(3352..4086)
                     /gene="trmD"
                     /locus_tag="VFR00_13910"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015931594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEU0160412.1"
                     /translation="MSIRSPAVLPTPWHVTVLTLFPELFPGPLGASLCGEALRQARWT
                     FETVDLRRFGRGRHRSVDDTPAGGGAGMVLRADVVAAAVDWARGQHPAAEAIYLSPRG
                     ERFTQETAQRLSQGPGVLLLAGRFEGVDQRVIEARGLREISIGDYVLAGGELPAMVLI
                     DAVVRLLPGVVGQPLSLAEESFAAGLLEYPQYTRPREWEGRSIPAVLTSGDHARIARW
                     RRSEAERLTATRRPDLWRQHTAGKDG"
     gene            complement(4202..4933)
                     /gene="pyrF"
                     /locus_tag="VFR00_13915"
     CDS             complement(4202..4933)
                     /gene="pyrF"
                     /locus_tag="VFR00_13915"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003611518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEU0160413.1"
                     /translation="MASIAARERLIVALDYAQIEDARELVRRLGESVGFYKIGLELVM
                     HGGLDFARELTGGGKQVFLDMKLLDIENTVERATRSAAATGASFLTVHGHDRKTVRAA
                     MAGKAGTPLRVLAVTVLTNLTATDLAEQGLSEAPEAVVARRARLAHSAGCDGIVAAGG
                     EAAAVRALVGPGVAIVTPGIRLASDAAGDQARITPPGTAILAGADYLVVGRPISGAAD
                     PRRAVAAFVAEIDAALARRALGPPS"
     gene            complement(5050..6282)
                     /locus_tag="VFR00_13920"
     CDS             complement(5050..6282)
                     /locus_tag="VFR00_13920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018701001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEU0160414.1"
                     /translation="MSRPQQVDVAIGGGGNVGLALAVALTDALGPGFTVALIDRAPWP
                     AAPGNDVRASALALGSKRLLTALGVWPHLAEFAQPVTAIDITDSRLGDAIRPILLSYD
                     NTLNDQEPASYIVENDRLRAALIAAAGAHRGLSLIGGVALKGFAADAAGVHIDLAEAT
                     LSARLLIGADGRGSPLREEAGIKVLRWSYPQTGIVTSVGHSRPHGGRALQHFLPSGPF
                     AILPLRHNRSCITWSEDTSEAGRLLALDQAAFREELARRFDYRLGEILEVGARAAWPL
                     EMQLARTLTAERFALIGDAAHLVHPIAGQGLNLGLRDVAALTEVVAEAARLGLDIGSG
                     LVLERYARWRRLDAALSAGTFDALNTLFSNDWTLVRTARDVGLGLVDRFPALKAFFVA
                     EAAGLTGEVPKLLRGERP"
     gene            6372..7271
                     /gene="tesB"
                     /locus_tag="VFR00_13925"
     CDS             6372..7271
                     /gene="tesB"
                     /locus_tag="VFR00_13925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013950213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006637 - acyl-CoA metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HEU0160415.1"
                     /translation="MSAEIPGAASAVARLLSILDLEPLEDNLFRGRSPQQSWQRVFGG
                     QTLGQALVAAVRTVPPERVAHSLHAYFLLPGDLDRPIIYSVERVRDGASFTTRRVTAT
                     QHGRAMFVMSVSFHKQEEGLEHQSAMPQVPPPEELPSEQDLKARMLAQLPEVMRKYWE
                     QERPIELRPVDVSRYFSREPRPAEQMVWLRASGRLPDAFPLHQCVLAYASDFSLLDTA
                     LIAHGKLMFDKDIQLASLDHALWFHRPFRADEWLLYAQDSPSSHGARGFCRGSVYRRD
                     GTLVASVAQEGLTRSRLSAFVPR"
     gene            7488..7826
                     /locus_tag="VFR00_13930"
     CDS             7488..7826
                     /locus_tag="VFR00_13930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEU0160416.1"
                     /translation="MKLVTAIIKPFKLEEVRSALTDLGLQGMTVTEVKGYGRQKGHTE
                     IYRGAEYAVSFLPKIKIEVVVPEGTVDKAVEAIVRTARTGQIGDGKIFVSPIEHAVRV
                     RTGETDDSAI"
     gene            7883..>8622
                     /locus_tag="VFR00_13935"
     CDS             7883..>8622
                     /locus_tag="VFR00_13935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003567452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia channel protein"
                     /protein_id="HEU0160417.1"
                     /translation="MSLGMPRRIATLAVAAFIVTLAAASAFAQTPAPPTPNKGDVAWM
                     LTASVLVLLMTVPGLALFYGGLVRTKNMLSMLMQVFFVVCMVCIIWVLYGYSLAFSEG
                     GSLNAFVGGFSKAFLAGITPDSTAETFTDGVVLPEYVYICFQMTFACITPALIVGAMA
                     ERMKFSALVLFIPLWVTFVYFPVAHMVWYWAGPAATVGAAKTLAAATGDAKAAAQAAM
                     DAVTANAGLAFQWGALDFAGGTVVHINSG"
BASE COUNT         1421 a         2901 c         2953 g         1347 t
ORIGIN      
        1 ctgtcctggc gggtggacat ccgtcggctg ttccctagcg ttagtcgggc ttgaccctac
       61 gagcgtggct ttgggcccga cccttgttat ggaaaagcgt ggccgtgcac aagcctttga
      121 cgcatcggcc ctcaacgcca atcgcctagc gtggcgttca cgagcgcgag agcggcaacg
      181 gcggccgtat ccgcgcgcag aatgcgcggg ccgagcgaga tgctgagcgc gaatggctgg
      241 gatgtgatga gctcacgctc gcccgcgtcg aagccgcctt ccggtccgat cagtaaggcg
      301 atgggtccgg gggccaggct ccgcagggcg gctaggggat cggccgcttc gctgtcctcg
      361 tcgcaaaaca ccaggtgccg ctcgacctgc caatgcgcca gcgccgcgcc gagcgtctcg
      421 ggttcgagca cctccggcac gcgcaggata ccgcactgct cggccgcttc gatggcattg
      481 gcgcgcatgc gcgcaccgtt gatccgctcg ggcgtggtgc ggcgggtaaa gacggggcgc
      541 aggcgggcaa cgcccatctc ggtcgccttt tgcaccatgt agtcgagccg cgcgcgcttc
      601 aagggtgcga acagataatc gatgtcgggc gcgccctgtt ggggccgtac ctgcgactcg
      661 gcgtgaagcg tcatcgcccc cttgcgcgcc tcgccaatgc tggcttgcca ttcgccatca
      721 cgcccgttga aaacgagaat ccgatcgccg gcattaaggc gcagcacatg gcgcagatag
      781 ttgacctgat cggggcgaca ggtcaccagc ttgccaggcg ccagatcggt ctcgacgtag
      841 aggcggctgg cagtgaagtc gtagcggcgc atggcatcct ccggcggcct tgagccggac
      901 gcgccgccgg cctctcgggc ctcggcccta aaccggcacg cccagcatca agccgatgtt
      961 ttgcagggct tggccggccg cgcccttacc gaggttgtcg agccgcgcca ccagcacggc
     1021 ttggcggtac ttggcgttgg cgaagaccct gagctcgagg ccattgctgc cgttgaggcc
     1081 ttcgggctcg aggcgcggcg gctgctccgc cggttccacc gtgggcagca ccttgacgct
     1141 cgtgcccgca tagtaacggg ccaaggcggc ctccagatcg gccgcgctcg gctcgcccgg
     1201 cagcgtgtcg aggtgcaggg gcacgctcac cagcatgccc tggcgaaagt tgccgaccga
     1261 aggcacaaac agcggccgcc gcgtcaactt ggcgtaggtt tggagctctg gcacgtgttt
     1321 gtgttcgagg ttgaggccgt agagctcgaa aacgggggcg tcaccggctt cgtagccttc
     1381 aatcatgctg cggccaccgc cgctgtagcc gctgaccgcg ttcaccgtca ggggatagtc
     1441 gcaaggcagc aggcccgcct ctaccagcgg gcgcacgagg gcaatggcgc ccgtcgggta
     1501 acagccaggg ttgctgacgc gacgggcttg gcgcacggcg gcggcctgct ctttgctgag
     1561 ttcgggaaag ccgtagaccc acccctcggc cacacggtga gccgtgctgc catcgacgat
     1621 cctgggtccg ccgcccggca aagaggccat gagcgcaacc gcctccttgg cggcggcatc
     1681 aggcaggcag aggacgacca ggtcgacctc gcgcatgatg gccagccgag cgcgcggctc
     1741 cttgcgctcg gcgggggcca cgctcttcac ctcgaggctc ggcacggcct cgagcatgcg
     1801 gcggatctgg aggccggtgg tgccggcctc cccatcgatg aagacggcgg gccttttggc
     1861 cgtggcaagg gcggctctgg gctcggaggc tatcttggtc ggcatggtct taatctcgag
     1921 gcagaataga caacaaaaaa gccgcgcttg gcacgcggct ggcgaagcgg acgcccgtct
     1981 cgacgagcgt catcgcagcc gcgggtcgaa ccggcggcgt cggcgggcga tcttcgcata
     2041 cctcgtcatg gccgctctta tggggtgcgg cgacgaacct gtcaatgccc gcaaccgccg
     2101 ccgtcaccgc gcgcctcaag ggcgctccgc ctggaggttg gcagcatcgg cacgagtagg
     2161 gccgctaagg gcaccgtgct cagcacagcg acggcaatca gccccatggc ggtggcgacg
     2221 ccgaagccat cgccgatcag gccaatgagg gggggcatgg ccgccgaggc ggcaatggcc
     2281 ccggtgtaga aaatgccgaa ggcgcgcgtg cggcgcgccg gtgccaccag ttcgggcacg
     2341 gtgccataga gcgccgagga ggttccattg aggccgatgc cgagcagcgg catgagcatg
     2401 aacgcccccg ccatcggtaa cgggatgagg gcgatgatgc cgatcgcggt gaggccttcc
     2461 gtcaacagca cggtgttgaa gagcccgacg cgcgccccga gataaccgca caccaacttc
     2521 cccgccgccc cacccacgaa cagcaaggag agcgcgctac cgatgctggc gatcgaggcc
     2581 tccttggcct tgagcagaaa gggcagaaag gtcagaaagc cgctgcggcc ggcgctgtcg
     2641 atcatggcga tggccagcag cagcaggaag ccctgacgcg aggggacctc ggccgcgctc
     2701 gctgcggctc gaagcggggc gacagcctcg tcgtcccggc gcgatgcgtt cgcctgcgcc
     2761 ggcgccaggg ccagcacgag gaccgcgcag gcgatgccga gaccgccaag cacccagagc
     2821 gccgagcgcc agggcatggt gctcagcagc agcgcggcca agagcggcat cgccattttg
     2881 ccgagatcgc cggtgaagtt atagacgccg agggccgtgc gggagcgtgc gccgctaaag
     2941 gcggccgaca ccaggcttga gccgatcggg tgttgcacgc tggcgccgat gccgcccaga
     3001 acgaggacca agagcagact gacgaggccc agcgaggcgc cggcgagcaa aaaggcaagc
     3061 gccgacacaa gcgtgccggc aaccaacagc aaaaccgggc cgcagcgttc ggccagcagc
     3121 gcggccggga tctgcagcgc cgccatcacg ccggtgtaac tgctgcgcag gaggccgacc
     3181 tcggcatacc ccaaaccgaa ttccgcctgc caaatcggca gcagcaggta aagcaggtcc
     3241 gtgtagccgt cgtgcaggat gtgcgcgcta cccgcggccg ccagcgtccg ccgctcctcg
     3301 cccggggcgc ttgggcgcgt gccggcatga gctttgttgc ttgtcatgga gctaaccgtc
     3361 cttgccggcc gtgtgctgcc gccaaaggtc ggggcgccgc gtcgcggtca gtcgctcggc
     3421 ctcgctgcgg cgccagcggg cgatgcgcgc gtggtccccg ctcgtcagca ccgcggggat
     3481 gcttcggcct tcccactcgc gcggccgtgt gtactgagga tattcgagca ggccggccgc
     3541 gaagctttcc tcggccagcg acagcggttg gcccacaacc cccggtaaca gccgcacgac
     3601 cgcgtcgatc agcaccatgg caggaagttc gccgccggcc aggacgtagt cgccgatcga
     3661 gatctcgcgc aggccgcgcg cctcgatcac gcgttgatcg acgccctcga agcggccggc
     3721 cagcaacagg acgccgggcc cttgcgacag gcgctgggcc gtctcctggg tgaagcgttc
     3781 gcctcggggg gagaggtaaa tcgcctcggc ggcgggatgc tggccgcgcg cccagtcgac
     3841 ggcggcggct acgacgtcgg cgcgcagcac catgccggcc ccaccaccgg cgggggtatc
     3901 atccacgctt cggtgccgac cccgaccgaa gcggcgaaga tcaaccgtct cgaaggtcca
     3961 gcgcgcctgc cgcaaggctt caccacagag cgaggcgccg agcggaccgg gaaagagttc
     4021 ggggaacagc gtcagcacgg tgacgtgcca gggtgtgggc agtacggctg gggatcgaat
     4081 ggacacggaa agaagacaga gatggttagg ggctcgctgc gggccgcaga ccgacacttc
     4141 atcccacgcc ggccctcgcc accgcaaggg cgacggcggc gctcgcccga cgccggcgag
     4201 ctcagctcgg cggtcccaag gcacggcggg cgagggcggc atcgatctcc gccacaaaag
     4261 ccgccaccgc ccggcgcggg tccgcggcgc cgctgatcgg ccgtccgacc accaggtaat
     4321 cggcaccggc cagaatagcg gtgccaggag gggtgatgcg cgcctgatcc ccggctgcgt
     4381 cgctcgccaa gcgaatgccg ggggtgacga tggcgacgcc gggtcccacc agcgcccgca
     4441 cggccgccgc ctcgccaccg gcggcaacaa ttccatcgca gcccgcgcta tgggcaagcc
     4501 gcgcgcgtcg cgccaccacg gcctccggcg cctcgctcag tccctgttcg gcgaggtcgg
     4561 tggcggtgag gttggttagg accgtcacgg cgagcacgcg caagggcgtg cccgccttgc
     4621 cggccatggc ggcgcgcacg gtcttgcggt catggccgtg cacggtcagg aagctggcgc
     4681 ctgtggccgc ggcgctgcgc gtcgcccgct ccacggtgtt ctcgatgtcg agcagcttca
     4741 tgtcgagaaa cacctgcttg ccccctcccg tgagctcacg ggcaaagtcg agaccgccgt
     4801 gcatgacgag ctcgaggcca atcttataaa aaccaacgct ctccccgagc ctccgaacga
     4861 gctcgcgtgc gtcctcgatc tgggcgtagt cgagcgcaac gatcagccgc tcgcgggctg
     4921 caatgctggc catggctctt ctcccggcgc gtggcgcggt tggctctccc ctgtccatct
     4981 tggacccggc gttgcgccca acacaagccg aggcccgagg tgctgcggag gtcgccaaag
     5041 cggagccgcc tagggacgct cgccgcgcaa tagcttcggg acctcgcccg tcaggccggc
     5101 ggcttccgcc acaaagaacg ccttgagcgc ggggaaacgg tcgaccaggc caagcccgac
     5161 atcgcgggcc gtccgcacca gcgtccaatc gttggagaag agggtgttga gggcgtcgaa
     5221 ggttccagcc gacagcgccg cgtccaaccg gcgccagcgc gcatagcgtt ccagcaccag
     5281 ccccgagccg atgtcgagcc cgagccgggc tgcctccgcg acgacctcgg tgagcgcagc
     5341 gacgtcgcgc aagccgaggt tgagaccttg cccggcgatc ggatgcacga ggtgggcggc
     5401 gtcgccgatg agggcaaagc gctccgccgt cagcgtgcgg gcgagctgca tttcaagggg
     5461 ccaggccgcg cgcgcgccaa cctcgaggat ctcgcccagc cgataatcga aacgccgtgc
     5521 aagttcctcg cggaacgcgg cttggtcgag cgccaacagc cggcccgcct cgctcgtatc
     5581 ttcgctccag gtgatgcagg agcgattgtg ccgtaacggc aagatggcaa agggacccga
     5641 cggcagaaag tgctgcagcg cgcggccgcc gtggggcctg ctatggccga cgctggtgac
     5701 aatgccggtt tgcgggtagc tccagcgcag gactttgatg ccggcttcct cgcgcagcgg
     5761 cgagccgcgg ccgtccgcgc cgatcagcaa gcgggcgctc agcgtggctt cggccaggtc
     5821 gatgtggacg cctgcggcat cggcggcgaa gcctttgagc gctacgccgc cgatgaggct
     5881 gaggcctcgg tgcgcccccg cggcggcgat cagcgccgcg cgcaagcggt cattctcgac
     5941 gatatagctg gctggctcct ggtcgttgag cgtgttgtcg taagacaaca ggatgggccg
     6001 gatggcatcg cccaggcgcg agtcggtgat gtcgatggcg gtgaccggct gggcgaattc
     6061 ggcgaggtgc ggccagacgc cgagcgccgt taagagccgc ttggagccca acgcgagcgc
     6121 cgaggcacgc acgtcgttgc cgggggccgc gggccaaggg gcccgatcga taagcgccac
     6181 cgtgaagccc gggcccaggg cgtcggtcag cgccacggca agggcaaggc caacgttgcc
     6241 cccgccgccg atcgccacgt ccacctgctg aggcctgctc atgcatcctt ccgaggcttt
     6301 aggggtgggg gcaccgcgcc tgctggcccg acagcttgac acctcgcgcg cgatgggcca
     6361 ggggcttgcg catgtccgcc gagatcccag gcgcagccag cgccgtcgcc aggctgctca
     6421 gcatcctcga cctcgagccg ctcgaggaca acctgtttcg cggccgcagc ccgcagcagt
     6481 cctggcaacg cgtgtttggc ggccagacgc tgggccaggc gttggtggcg gccgtgcgta
     6541 cggtgccgcc cgagcgggtc gcgcattcgc tgcatgctta tttcttgctc ccgggcgacc
     6601 tcgaccgtcc cattatctat agcgtcgagc gcgtgcgcga cggcgcgagc ttcaccacac
     6661 ggcgtgtcac ggccacccag cacggccggg cgatgttcgt catgagcgtg tcgttccata
     6721 agcaggagga ggggttagag caccaaagtg ccatgccgca ggtgccaccg cccgaggagt
     6781 tgccgagcga gcaggacctg aaggcgcgca tgctcgccca gttgccggag gtgatgcgca
     6841 aatactggga gcaggaacgg cccatcgagc tgcgaccggt cgacgtctcg cgctacttct
     6901 cgcgcgagcc gcgccccgcc gagcagatgg tgtggctgcg ggccagcggc cgcttgcccg
     6961 atgcctttcc cctgcaccag tgcgtgctgg cctacgcatc ggacttttcg ctcttggaca
     7021 cggcgctgat tgcccacggc aagctgatgt tcgacaagga catccagctc gcgagcctgg
     7081 atcatgcctt gtggttccac cgtccgtttc gggcggacga atggctgctc tatgcccaag
     7141 atagcccctc gagccacggc gcgcgggggt tctgccgcgg cagcgtttac cgccgcgacg
     7201 gcaccctcgt ggcctcggtg gcgcaagagg gcttgacccg ttcgcgcctc tccgccttcg
     7261 tgccccgctg acgcgcacga agcacttctt gcctaaattt taggcaggcc gcgggccctc
     7321 gcgcgggcct gacgattccc gtttcataag tgtttccaga ggcttatggg atgggcaggc
     7381 agttggcacg gctcttgact attaaccctt tgcgccgctg agcgagcggc gtgggatgcc
     7441 gcgtgctcag tcgatgagca aggcggccgg agcataaggg gaaccagatg aagctcgtaa
     7501 cggccatcat taagccgttc aagctcgagg aagtccgctc ggcgctgacc gatctcgggc
     7561 tgcaaggcat gaccgtgacc gaagttaaag gttacggccg gcagaagggg cacacggaaa
     7621 tctaccgcgg ggcagagtac gcggtgagtt tcctgcccaa gatcaagatc gaggtggtgg
     7681 tgccggaagg caccgtcgac aaggccgtcg aggccatcgt gcgcactgcc cgcacgggtc
     7741 aaatcggcga cggcaagatc ttcgtgtcgc cgatcgagca cgccgtccgc gtccgtacgg
     7801 gcgaaaccga cgacagcgcc atctgacctt ggcgctcctg caacgattag aacccgcccg
     7861 cagtctcttg gaggcaccac tcatgtcact tggtatgcca cgccgaattg ccaccctggc
     7921 agtcgcggcg ttcattgtca cacttgccgc cgcatcggcg tttgcgcaaa cgcccgcgcc
     7981 gccgaccccc aacaaggggg acgtcgcctg gatgctgacg gcttccgttc tcgtcctgtt
     8041 gatgacggtg ccaggacttg cgctgttcta cggcggattg gtgcgcacca agaatatgct
     8101 gtccatgttg atgcaggttt tcttcgttgt ctgcatggtc tgcatcatct gggtgctcta
     8161 tggctatagt ctggcctttt ccgaaggcgg ctcgcttaac gccttcgtcg gcggcttctc
     8221 caaggccttc cttgccggca ttacgcccga ttcgacggcg gagaccttca ccgacggcgt
     8281 cgtactgccc gagtacgtct acatctgctt ccagatgacc tttgcctgca tcaccccggc
     8341 gcttatcgtc ggcgcgatgg ccgagcgcat gaaattctca gccctcgtcc tatttattcc
     8401 attgtgggtc accttcgtgt acttcccggt cgcgcatatg gtctggtatt gggccggccc
     8461 cgcggccacg gtcggtgcgg ccaagacgct ggctgccgcc accggcgatg ccaaggccgc
     8521 cgcccaggcg gcgatggacg cggtaaccgc caacgccggc ctcgcctttc agtggggcgc
     8581 gctcgacttc gccggcggca ccgtggtgca tatcaactcg gg
//
LOCUS       DASTMT010000607         3619 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_4820813, whole genome shotgun sequence.
ACCESSION   DASTMT010000607 DASTMT010000000
VERSION     DASTMT010000607.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3619
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4820813"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..418)
                     /locus_tag="VFR00_13940"
     CDS             complement(<1..418)
                     /locus_tag="VFR00_13940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020607809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU0160418.1"
                     /translation="MTSQHHLFGPPELDRLFRPVLGEPARGLALAVSGGSDSTALMLL
                     VADWLALTRQDPRAVFVLTVDHGLRPEAASEARWVEQAAVRLSFRHAVLPWRGPKPKT
                     GLQAAARAARYALLAEFMRAAGLSRLVTAHTADDQAE"
     gene            complement(658..1752)
                     /gene="ybgF"
                     /locus_tag="VFR00_13945"
     CDS             complement(658..1752)
                     /gene="ybgF"
                     /locus_tag="VFR00_13945"
                     /inference="COORDINATES: protein motif:HMM:TIGR02795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HEU0160419.1"
                     /translation="MNLRLARVAIAGPALVTAALVAMSVAPGLAQQGPAPTQRPLPPP
                     QPAPKAAQPRDTGQAGEAALRQRIEQLEEQLVDMQVVVGTLESLAKGAPAPVSGGLAG
                     RGNPGAAAALNAADAGRLDSLETQIQALAAQLENLQEQVRALVGRSGAVAPSSSFGAA
                     EPPAKAPRFAETNPPPVAGRAGFGAVTVSPGVVPGQRDDIDQLLTGQPGAPPPGAAES
                     AGGTRVAAAPLPAAAPEQDVVPKQIYETAYGYLLQRDYGAAEAAFEDFLQRFPNDPLA
                     GNAQYWLGESLFVRGQYRSAAGAFLKGYQNYGRNAKAPESLLRLAMSLQRLGQKDAAC
                     SSFNELASKYPSAPPHVRTSAQAERQKAGC"
     gene            complement(1967..2488)
                     /locus_tag="VFR00_13950"
     CDS             complement(1967..2488)
                     /locus_tag="VFR00_13950"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEU0160420.1"
                     /translation="MSLVFAGPSSAQERMHLGVPDGVAAGVGRDFRMSVGDRVFFADR
                     SAELSSRARAAIDAQAQWLKRKPGLSITVEGHADDSGTSDDNMALSRQRADAVRYRLI
                     ELGVGTERIRISAFGRNQTVADCTGVQCAAQNRRAITVVSPGLPVPVSTPGAARKAAA
                     TGAPWPRTPAPVF"
     gene            complement(2669..3175)
                     /gene="pal"
                     /locus_tag="VFR00_13955"
     CDS             complement(2669..3175)
                     /gene="pal"
                     /locus_tag="VFR00_13955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HEU0160421.1"
                     /translation="MRLSRWHRAGGPTLAAVAVALLLVACSNTPKPESELGRNSPATP
                     GSSRDFAVNVGDIVYFSTDSTDLSPEAQQTLTNQARWLQQYAQYSITIEGHADERGTR
                     EYNIALGARRAQTVRDFLARNGINAGRIRTISYGKERPVAVCNDISCWSQNRRAQTVL
                     NGRAEARS"
BASE COUNT          583 a         1219 c         1240 g          577 t
ORIGIN      
        1 tctcggcctg atcgtcggcg gtgtgcgccg tgaccagacg cgagagcccg gccgcgcgca
       61 tgaactccgc caacaaggca tagcgcgcgg cgcgcgccgc cgcctgtagg ccggttttcg
      121 gcttgggccc gcgccaaggc aggaccgcgt ggcgaaagct gagccggacg gccgcttgct
      181 ccacccaacg ggcctcgctc gcggcctcgg gcctcagacc atgatcgacg gtcagcacaa
      241 agacggccct tgggtcttgc cgcgtcagcg ccagccagtc cgccaccagc agcatgaggg
      301 cggttgagtc gctgccgccc gagacggcga gcgcgaggcc gcgcgcgggc tcgccgagca
      361 ccgggcgaaa cagccgatca agttcaggcg gcccgaagag gtgatgctgc gaagtcataa
      421 tccgcatgcg cctggggacg atcggccggt gccacctgac gggcaccgac ccaggtgcac
      481 cctgagcggt gaaccgaaca ggaaggatgt tagcagccgg ggcacgccgc ctctagtgac
      541 ggaagccccg aacgtcccgc gcgcttggac cttccctcgc cctgcccggc agtgcgggcg
      601 cgggcacgcc accaggcgcg ggctgaggcc gtaccgggtt ggcgcggcag tgaatcctca
      661 gcagccggcc ttttgtcgct cggcctgggc actggtgcgc acgtgcggcg gcgcgcttgg
      721 atacttgctt gccaactcgt tgaaggagga gcaggccgca tccttctggc ccaagcgttg
      781 cagcgacatg gcgagccgca gtaggctttc cggcgccttg gcgttgcgac catagttttg
      841 gtagcccttc aagaaggcac cggccgccga gcggtattgc ccacgcacga acagcgactc
      901 gcccagccaa tattgggcat tgccggcgag cggatcgttg ggaaagcgct gcaggaaatc
      961 ctcgaaggcg gcttcggcgg cgccatagtc gcgctgcagg aggtagccgt aggcggtttc
     1021 gtagatctgc ttgggcacca catcctgctc cggcgcggcg gcgggaagcg gggcggcggc
     1081 gacgcgcgtg ccgccagcgg actctgccgc gccgggcggc ggcgcacccg gctggcccgt
     1141 cagcagttga tcgatatcgt cgcgctggcc cggcaccacc cccggggaga cggtcacggc
     1201 gccgaagccg gcacgccccg ccaccggcgg cgggttggtt tcggcgaagc gaggagcttt
     1261 cgccggcggc tcggcggcgc caaacgacga ggagggagcg acggccccgc tgcggccgac
     1321 cagggcgcgc acctgctctt gcaaattctc aagttgggcg gcgagcgctt ggatttgggt
     1381 ttcgaggcta tcgaggcgcc cggcatcggc ggcattgaga gcggcggcgg cccccgggtt
     1441 gccgcgtcct gccaaccctc cgctcaccgg tgctggcgct cccttggcga gcgattcgag
     1501 cgtccccacg accacctgca tgtcgaccag ctgttcctcc agctgctcga ttcgctgccg
     1561 cagcgccgcc tcgcctgcct ggccggtatc gcggggttgg gcagccttcg gtgccggctg
     1621 tggcggcggc agtggccgct gcgtgggggc ggggccttgc tgcgccaagc ctggcgcgac
     1681 agacattgcc accagcgctg ccgtcacgag cgccggcccg gcaatcgcaa cgcgtgctaa
     1741 tctcaggttc atggcttcgt cccgcctgcg cgctttccgc cccttcaaca cgaccttccg
     1801 ggcaggagca agactgcggc cctgagatcg aaagaaaaac tcgaaaaagc ccagcggcgc
     1861 cgcaaaacgc ggcgcctaag ctttgccccg ccaaggcgcc ggcattaggt cgcctcgagc
     1921 caatccggct gatcctctcg cgcgggtgcg ccagccaaca ccgcccctag aacaccgggg
     1981 ctggggtgcg cggccagggc gcccctgttg ccgccgcctt gcgcgcagca ccgggcgtgc
     2041 tcaccggaac cggcagcccc gggctgacga cggtaatggc acggcggttc tgtgccgcgc
     2101 attgcacgcc cgtgcagtca gcaacggtct gattgcggcc gaaggcggaa atccgaatgc
     2161 gttccgtgcc gacaccgagt tcgatcagcc ggtagcgcac ggcgtcggcg cgctgacgcg
     2221 acagcgccat gttgtcgtcg ctggtgcccg aatcgtcggc gtggccttcc acggtgatgc
     2281 tgaggccggg tttacgcttg agccactggg cctgtgcgtc gatggcggca cgcgcgcggc
     2341 tgctgagctc ggcactgcgg tcggcaaaaa acacccggtc gccgacgctc atgcgaaagt
     2401 cccggccgac tccggccgcc accccatccg gcaccccgag gtgcatgcgc tcttgcgccg
     2461 agcttgggcc ggcgaacaca agggacatcg tgccggccaa aataacggcc aggccgagtg
     2521 atcgaccgca tggcatatgg cgtgctcccc cacgcttgat gcatcacggc cacactgcgc
     2581 ccgcatttag gccaaaattt ggtgacgggg ggctccagcc gccgcgcagc cgccagcgac
     2641 ggcttgcaag ccgaggagat tcggggcctc aggagcgcgc ttcggcgcgg ccgttgagga
     2701 cggtctgggc gcgccgattc tgcgaccagc acgagatatc gttgcacacc gccacggggc
     2761 gctccttgcc ataggagatg gtgcggatgc gcccggcatt gatgccgttg cgggcgagga
     2821 agtcgcgcac ggtctgggcc cggcgggcgc ccagggcaat attgtactcg cgcgtgccgc
     2881 gctcgtcggc gtggccctcg atggtgatcg aatactgggc gtactgctgc agccagcggg
     2941 cctgattcgt cagggtttgc tgggcctcgg gcgacagatc ggtgctgtcg gtggagaagt
     3001 agacgatatc gccgacattg acggcaaagt cgcgggagga gcctggcgtg gccgggctgt
     3061 tgcggcccag ttcgctttcc ggcttggggg tattggagca ggcgaccagc agcagggcca
     3121 cagcgacggc tgccagtgtg gggccacccg ctctgtgcca cctcgacagc cgcatgccgt
     3181 tccccttggt gttgcggccg acggcgcttg cgccgcaagc cgattgccga tcaggttgca
     3241 atatctagca aaagcttcgt tcaaaaatgt gtccgccgcc gcgccgtgct ggtcaaacgt
     3301 gctttcggcg gatcagacaa ccgggcgcgg cgcaatttcc ccgcaataca cagcccagca
     3361 gatcttaaaa acaaccaggg ctgtagcaag gcagacatcc tgccgcgcta cagccccaat
     3421 cctgcgattc gtctgccaaa gggcagcgat cgctccggct caacgagcga cgtcttgcac
     3481 gctcgcgccc agcttcatcg gtgtatgctc tacttctttc cggcatgcgc cgagagcgac
     3541 agcagcgatg acagctgcga gaacgatagc gcccttcatg cttcttctcc taaggtctct
     3601 tagagagttg gtgttaacg
//
LOCUS       DASTMT010000608         5642 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_5151099, whole genome shotgun sequence.
ACCESSION   DASTMT010000608 DASTMT010000000
VERSION     DASTMT010000608.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 5642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5642
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5151099"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..367)
                     /gene="ruvA"
                     /locus_tag="VFR00_13960"
     CDS             complement(<1..367)
                     /gene="ruvA"
                     /locus_tag="VFR00_13960"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002964792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEU0160422.1"
                     /translation="MIGKLKGLVDAIGEAHAIIDVGGVGYEVQASARTLRQLNLGQEV
                     SLAIETHVREDAIRLFGFVSEVERAWFRTLQTIQGVGAKVALGVLGTLGPQDLANAIA
                     LQNWAAVEQAPGVGKKLAQR"
     gene            complement(475..1014)
                     /gene="ruvC"
                     /locus_tag="VFR00_13965"
     CDS             complement(475..1014)
                     /gene="ruvC"
                     /locus_tag="VFR00_13965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020186394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HEU0160423.1"
                     /translation="MATHPIRIIGIDPGLRRTGWGIIVAEGARLSYVASGLITSDSEE
                     PLAYRLRALHAGLIEVITRFAPEEAAVEETFVNKDARATLKLGQARGMALLAPAQRGL
                     RVAEYPPNVIKKTITGTGHGDKQQIAAMVGFLLPRAKFDSADEADALAIAVCHASHRA
                     SYRLGQAAGAQRALPEAAP"
     gene            complement(1092..1841)
                     /locus_tag="VFR00_13970"
     CDS             complement(1092..1841)
                     /locus_tag="VFR00_13970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011582505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEU0160424.1"
                     /translation="MAGHSQFKNIMHRKGRQDAAKAKVFAKLAREITVAAKAGLPDPE
                     MNARLRLAIQSARAENMPKDNIERAIKKAAGADTESFEAMRYEGYGPGGVAILVETLT
                     DNRNRTGGAIRALFAKHGGNLGATGAVAHMFAHIGEITYPSAAGSPDHLLEAAIEAGA
                     EDVLSGVSGHVVTTGFADLGTVSAALEAKLGEAQTVKALWKAVQTTSVDEDKAASVLK
                     LIAGLEEEDDVQSVYSNFEVSDAVMAKLTAA"
     gene            complement(2251..3075)
                     /locus_tag="VFR00_13975"
     CDS             complement(2251..3075)
                     /locus_tag="VFR00_13975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HEU0160425.1"
                     /translation="MRLLFIGDVVGRSGRKVVVDELPRLRARYGLDFVAVNAENAAGG
                     FGITESILEDLLSAGADVVTLGNHAFDQKEALVFIERQPRLLRPINYPPGTPGKGSGL
                     FKAANGADVLVINAMGLIFMPELGCPFRAVDAEVVACGLKRGADAILLDFHAEATSEK
                     QAMGYFLDGRASCVVGTHTHVPTADERVLPQGTAFMCDVGMCGDYESVLGMDREEPLN
                     RFLSKIPRARFEPALGPGTLCGLAVEIDDATGLAVRAKAVRLGGVLAPTEPPFWVE"
     gene            complement(3079..3666)
                     /locus_tag="VFR00_13980"
     CDS             complement(3079..3666)
                     /locus_tag="VFR00_13980"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEU0160426.1"
                     /translation="MSATILEQKQALRVRAKADRAKLLPAQKVAAANVVAELGLSFLP
                     PLSPTATVSGFAPLADEFRLWPLLRRLAAEGVALALPVMQGKGKPLLFRAWQPGDQTA
                     SGVWGIAEPTPEKAQVEPDILLVPLLAFDLAGWRLGYGGGFYDRTLRRLRGMQPVLAV
                     GIAFDEQRVDAVPHLDYDERLDWVLTPSGPLRCVE"
     gene            complement(3712..3873)
                     /gene="ssrS"
                     /locus_tag="VFR00_13985"
     ncRNA           complement(3712..3873)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="VFR00_13985"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(4073..4342)
                     /locus_tag="VFR00_13990"
     CDS             complement(4073..4342)
                     /locus_tag="VFR00_13990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0160427.1"
                     /translation="MTPRRGDAASRKTAAARLQSERSNSKPTKPAAAGSADKLAALER
                     ERDALRLELERSEARVEALEKAQTQVRDRLAWALDSLQNIIQGKG"
     gene            4631..5635
                     /gene="gap"
                     /locus_tag="VFR00_13995"
     CDS             4631..5635
                     /gene="gap"
                     /locus_tag="VFR00_13995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010661401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEU0160428.1"
                     /translation="MAVRVAISGFGRIGRNILRAIVEAKRKDVEIVAINDLFSAKDNA
                     HLLRHDSVHGRFPGEVSVAGDSIDVGNGPIKVLAERDPHKLPWKSMDIDVVMECTGIF
                     TDRDKAAAHLEAGGKRVLVSAPAKGADLTVVFGVNHEKLTRDHKVVSNASCTTNCLAP
                     VAKVLNDLVGIKSGFMTTIHAYTNDQNVLDQAHKDMRRARAAALNLIPTSTGAAAAVG
                     LVLPELKGKLDGTAIRVPTPNVSVVDLKFIPGRETAVDEINKAMQHAAKQELKGILAV
                     SNEPLVSIDFNHDPHSSTFDLTQTQIVDGKLVRVLAWYDNEWGFSNRMSDTAALFGSL
                     "
BASE COUNT          992 a         1910 c         1787 g          953 t
ORIGIN      
        1 tgcgctgcgc cagctttttg ccgacccccg gcgcctgctc cacggcggcc caattttgca
       61 gcgcaatcgc attggcgagg tcctgcggcc ccagcgtccc gagcacgccg agcgccacct
      121 tcgcgccgac gccctgaatg gtttgcaagg tccggaacca ggcacgctcg acctcgctga
      181 caaagccaaa gaggcggatg gcgtcctccc gcacgtgcgt ctcgatggcg aggctcacct
      241 cctgccccag gttcagctgg cgcagcgtgc gcgcggaggc ttgcacctcg taaccgacgc
      301 cgcccacgtc aatgatggcg tgcgcttcgc cgatggcatc gaccaggcct ttgagcttgc
      361 caatcatcta cgccccgccc gcagccgtat aggcccgcgc gcggccccgc gggccaattg
      421 gccccgagag cccgtcttga ccccacgggc ctgccgcgca tcggcccgag cgcctcatgg
      481 cgcggcctcc ggcaaggcgc gctgcgcacc ggcggcctgg cccagacgat agctcgcccg
      541 gtggcttgca tgacacaccg cgatggcgag ggcatcggcc tcatcggcgc tgtcgaactt
      601 cgcccgcggc aacaggaagc caaccatcgc cgcgatctgc tgtttgtcgc cgtgccccgt
      661 gccggtgatg gtttttttga tgacgttggg ggggtattcg gcaaccctta gccctcgctg
      721 cgccggcgcc agcagcgcca tcccccgggc ctgacccagc ttcaaggttg cacgcgcatc
      781 cttattgacg aaggtctcct cgaccgccgc ctcctccggg gcaaagcggg tgatcacctc
      841 gatcagcccc gcgtggaggg cccgcagtcg gtaggccagg ggctcctcgc tgtcggaggt
      901 gataagcccg ctggccacat aggacagccg cgctccctcc gcgacgatga tcccccagcc
      961 cgtacggcgg aggcccggat cgatgccgat gatacgaatc ggatgcgttg ccatgctccc
     1021 cgatagcacg agcggtggcg ttttggcttc ggcgggttgc acccgcacgg gggcccttgg
     1081 ccgggccggg gttaggccgc cgtcaatttc gccatcaccg catcggagac ctcgaagttg
     1141 gagtagacgc tttgcacgtc atcctcctct tccagaccgg cgatcaactt cagcaccgag
     1201 gcggccttgt cttcgtcaac cgaggtggtc tgcaccgctt tccacagcgc cttgacggtc
     1261 tgggcctccc caagtttggc ctccagcgcc gccgagacgg tcccgagatc ggcaaacccc
     1321 gtggtgacga cgtggccgct cacccccgag agcacgtctt cggcgcccgc ctcgatggcg
     1381 gcctcgagca gatgatccgg cgagcccgca gccgacgggt aggtgatttc gccgatgtgc
     1441 gcaaacatgt gcgcgacggc gccggtggcg ccgagattgc caccgtgctt ggcaaacagt
     1501 gcccggatcg ccccgccggt gcgattgcgg ttgtcggtca gggtctcgac gaggatggcg
     1561 acgccgcccg gaccgtagcc ctcatagcgc atggcctcga agctttccgt gtcggcaccg
     1621 gccgcctttt tgatcgcccg ttcgatattg tccttgggca tgttctccgc gcgcgccgat
     1681 tgaatggcga ggcgcaggcg cgcattcatc tccggatcgg gcaacccggc cttggcggca
     1741 accgtgatct cccgcgccag tttggcgaag accttggcct tggccgcgtc ctggcggccc
     1801 ttgcggtgca tgatgttctt gaactgggaa tggccggcca tcgtgatccc aaggctgtca
     1861 gctcgcgcac cccgcgccgg ggcaaatttc gcccgaggcg gcgtcgcggc caggcgttgc
     1921 gctcctgcct gccacgactc accccctcaa tgccgccgcc tgcggcagtc aggggcctgg
     1981 gcccctggca tcgcgggccc cagcttttta cgcttatagg agcccgcttt gggcaaataa
     2041 agaggagtgg cgccggggcc gggaagggcg ttaagaattt gtttacactt agcccctagc
     2101 gttcgcgcca ggaatgcaaa tcgcattctg gatttgccgc cctgctgccc acggcgccag
     2161 atccagggag ccgcgagtac cagctctctt cccacaccac gaaggagacg gattggccgt
     2221 ctccctacaa caagactgac cctctctttc ttactcgacc cagaatggcg gctcggtagg
     2281 tgcaaggaca ccaccgagcc gcacagcttt tgcgcgcacc gccagccccg ttgcatcatc
     2341 gatctcgacc gccaggccgc acagcgtgcc aggaccaagt gcgggttcga agcgggcgcg
     2401 cgggattttg ctcaagaacc ggttgagcgg ttcctcccgg tccatgccca aaaccgattc
     2461 gtagtcgccg cacataccca catcacacat gaaggcagtg ccctgcggca gcacgcgttc
     2521 gtccgcggtg ggcacgtgcg tgtgggtacc gacgacgcag ctggcgcggc cgtcgagaaa
     2581 gtagcccatc gcctgctttt cgctggtggc ctcggcgtga aagtcgagga gaatggcgtc
     2641 ggcgccccgc ttcaagccgc aggccacgac ttccgcgtca accgcgcgga agggacagcc
     2701 gagttcgggc atgaagatta agcccatggc gttgatcacc agcacgtcgg cgccgttggc
     2761 ggccttgaat aagcccgagc ccttgccggg tgtgcccggc ggatagttga tcggccgtag
     2821 caaacgcggc tggcgctcga taaagacgag cgcttccttt tgatcgaagg catggttgcc
     2881 gagcgtcacg acgtccgcgc cggcgctcag taaatcctcg agaatggact cggtaatgcc
     2941 aaagccgccg gcggcgtttt cagcattgac cgccacgaag tcgaggccat agcgcgcccg
     3001 cagccttggc agttcatcga ccaccacctt gcgccccgag cgcccgacga cgtcgccgat
     3061 gaacagcagc cgcatgcctt attccacaca gcgaagggga cccgacgggg tcagcaccca
     3121 gtcgaggcgt tcgtcataat cgagatgcgg cacggcgtcg acgcgctgct cgtcaaaggc
     3181 gatgccaacg gcgagcaccg gctgcatgcc gcgcagccgc cgcagcgtgc gatcatagaa
     3241 gcctccgccg tagccgagcc gccagcccgc aaggtcgaaa gcgagtagcg gcacaagcaa
     3301 gatgtcgggc tccacttggg ctttctcggg cgtgggttcg gcaatgcccc aaaccccgga
     3361 cgcagtttga tcgccgggct gccaggccct gaacagcaac ggcttgccct tgccctgcat
     3421 caccggcaag gccaaggcga ccccctcggc ggcgagccgc cgcagcagtg gccaaaggcg
     3481 aaattcatcg gccagcggcg cgaagccgga aacggtggcc gtgggggaaa ggggaggcag
     3541 aaagctgaga ccgagttcgg ccacgacgtt cgcggccgca accttctgtg cgggcagaag
     3601 cttggcgcgg tcggccttag cccggacccg caatgcctgc ttttgctcga ggatggtcgc
     3661 gctcacaccg tcggcttagc gcaaatcgag ctccggtgcg agggcgagaa cagcagagcc
     3721 gcgaaagccg tgcgagcggc gatcccgcgc ggtccctacg agatgtaggt gggcgccgtg
     3781 tgtccgagac cacgatcccg gacagggaca gctcccgtgc ggatcttata ggcccccggg
     3841 tcgcattgct cgccacgcac cccgcagccc tgcagggcct gagattaggc cgcggccgtg
     3901 aaaaatcaat gtgcgaaaaa actgtgggcg cgcgggcggc gccgcaccga gagcgcttgc
     3961 tggccagcgt tggccgccgc cccaaactta ggggcgcggg ggcgccggca ttgggcaagg
     4021 cctagccagc ggccgtccgt acgctgccgc accgatggcg gcgggggcca tctcagcctt
     4081 tgccctgaat gatgttctgc agggaatcga gcgcccaggc caggcgatcg cgcacctgcg
     4141 tttgcgcctt ttcgagcgct tccacgcggg cctccgagcg ctcgagctcg agacgcaaag
     4201 cgtcgcgctc gcgctcgaga gctgccagtt tgtcggcgct gccggcggca gccggtttcg
     4261 tcggtttcga gttgctgcgt tcagactgca ggcgcgcagc ggcagtcttt ctgctcgcag
     4321 catccccgcg gcgtggggtc attgcataaa ccattgtttc ttgcgccgcc tgaaatacgc
     4381 ggcgcggccc tcgacgtttt cgacgatagg taggccggcg ggcagcgtca acgcattcaa
     4441 gattgcaatt cttggcccaa ctacatcaca ggcgtgaact gaaggccgat attgacaggg
     4501 ccgcgtgaag acaccactag aggcgggagt aagaggtgcc acccagaagc cacagcgctg
     4561 cggcgcgagc gtgcccagca gcattggcgt ttcgcgcgtt gccaacctcc cggaacgaat
     4621 ggagcagccc atggccgtta gagttgcaat ttccggtttc ggccgtatcg gccgcaacat
     4681 cctgcgcgcc atcgtggaag ccaagcgcaa ggacgtcgaa atcgtcgcca tcaacgacct
     4741 gttttcggcc aaggataatg cccacctgtt gcggcacgac agcgtgcacg gccgctttcc
     4801 cggcgaagtg agcgtcgccg gcgacagcat cgacgtcggc aatgggccga tcaaagtgct
     4861 ggccgaacgc gatccccaca agcttccctg gaaatccatg gacatcgacg tggtcatgga
     4921 atgcaccggc attttcaccg atcgcgacaa ggctgccgcg cacttggaag cgggtggcaa
     4981 gcgcgtgctg gtgtccgcac cggccaaagg tgccgatctg accgtggtgt tcggggtcaa
     5041 tcacgagaaa ctgacgcgcg atcacaaggt cgtgtcgaac gcgtcctgta ccaccaactg
     5101 cctggccccg gtcgccaaag tgctgaatga cctggtcggc atcaagtccg gttttatgac
     5161 gacgatccac gcctacacca acgaccaaaa cgtgctggat caggcgcaca aggacatgcg
     5221 gcgggcccgc gccgccgccc tcaacctgat cccgacctcg accggcgcgg ccgccgccgt
     5281 cggcctggtc ctgcccgagc tcaagggcaa actcgatggc accgccatcc gcgtgccgac
     5341 ccccaatgtc tccgtcgtcg atctcaagtt catccccggt cgcgagaccg cggtcgatga
     5401 gattaacaag gcgatgcagc acgccgccaa gcaggagctg aaggggattt tggcggttag
     5461 caacgaaccg ctggtctcaa tcgacttcaa ccacgatccg cactcctcga ccttcgacct
     5521 gacgcaaacc cagatcgtcg acggcaagtt ggtcagggtg ctcgcctggt acgacaacga
     5581 gtggggcttc tcgaaccgca tgtccgatac cgcggcgctg ttcggcagcc tgtagccgcc
     5641 tg
//
LOCUS       DASTMT010000609         5892 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_3831443, whole genome shotgun sequence.
ACCESSION   DASTMT010000609 DASTMT010000000
VERSION     DASTMT010000609.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 5892)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5892)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5892
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3831443"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            181..2964
                     /gene="fdhF"
                     /locus_tag="VFR00_14000"
     CDS             181..2964
                     /gene="fdhF"
                     /locus_tag="VFR00_14000"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015931687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HEU0160429.1"
                     /translation="MTTAAKIILELDGEPVEAVPGETIWQLAQRHGCKIPHLCWHPSP
                     GYRADGNCRACMVEIEGERVLAASCQRKAAAGMKVKTSSERAQKARQMVFELLLADQP
                     ERGDGHDPASKFWGWVDAMGIGTKGRFPAGERPPSDVSHAAIAVNLDACINCGLCVRA
                     CREVQMNDVIGMAYRGHGAKVVFDFDKAMGHSTCVACGECVQVCPTGALMERTLLDAK
                     GKRVNYETRSVDTLCPYCGVGCQTQVHVKDDKILYVDGRNGPANENRLCVKGRFGFDY
                     IHHPDRLKRPLLRRDGIGKSADLQIRRDEVDKYFREVSWEEAMAHAAAGLERIKRRDG
                     GTAIAGFGSAKGSNEEAYLFQKLIRQGFGTNNVDHCTRLCHASSVAALMEGVNSGAVT
                     APFTAAKDSDCIIVIGARPAENHPVAATFLKNAAKRGAKLIVMDPRGQSQGLARYASH
                     TLQFKPGRDVALLNAMLNVIIAEGMVDIQYVQAHTEGFDSLKAKVEAFTPEKMEAVCG
                     IPAATIRDVARLYARSERSIIFWGMGISQHTHGTDNARCLIALALVTGQLGRPGTGLH
                     PLRGQNNVQGASDAGLIPMVYPDYRSVEDPLVRNAYETFWGTALDPKKGLTVVEIMHA
                     IKDGSIKGMYILGENPAMSDPDVQHAREALAKLEHLVVQDLFLTETSWHADVILPASA
                     HAEKWGTFTNTNRQVQIARPVLNPPGEARQDWLLIQELAQRIGLPWNYRDISEVYTEM
                     AEVMPSLNNISWERLLREDAVTYPCDAPDKPGNEIIFAASFPTKSGRAKIVPADLLPP
                     DELPDADYPLVLTTGRLLEHWHTGSMTRRASNLDSLEPEAIAGLNPREMERLGVSAGG
                     WVRVSTRRGTVVLKARADRDVAEGMVFLPFCFAEAAANLLTNPQLDPMGKIPEFKFCA
                     AKVEPAAAAMAAE"
     gene            complement(2991..3938)
                     /locus_tag="VFR00_14005"
     CDS             complement(2991..3938)
                     /locus_tag="VFR00_14005"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEU0160430.1"
                     /translation="MVDRASSRRKTPEVEPASGPESATARPVPKFDKKTIALVYDFDG
                     TLSPRPMQEYAFLPKIGADPAAFWAESNALATQAHADPLITYMHLMYKKAKDKGVRID
                     RRDLVAQGRQVELFAGVESWFESIGVYVKSHAQSHGVQLRHYLISSGLSEIVEGTSIY
                     KRFHNVFASEYWFDAYDLPYPKRVITDTGKTQYLFRINKGVEDLGRSINQHMPEALRP
                     IPFANMIYFGDGDTDVPSMAVMRKNGGHAVAVYPPGKSKAKCVDLFNAGRCDFFAPAD
                     YRRGSDLYRRTCLLLDRMLANIRVEEERWRLGRGLPPAR"
     gene            complement(4124..4402)
                     /gene="infA"
                     /locus_tag="VFR00_14010"
     CDS             complement(4124..4402)
                     /gene="infA"
                     /locus_tag="VFR00_14010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011474121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEU0160431.1"
                     /translation="MAKEEMLEFEGVVAEVLPDARCRVKLDNGHEVVAYTSGRMKKNR
                     IRILAGDRVTVEMTPYDLDKGRINFRHKDVRAPVTSGPPRRPPQRRFR"
     gene            complement(4689..4916)
                     /locus_tag="VFR00_14015"
     CDS             complement(4689..4916)
                     /locus_tag="VFR00_14015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0160432.1"
                     /translation="MSTLTYTEADYAPGAGARAPVRGALWRRVYAAIMRSQQRRAERE
                     IAAYLDSHGGLFTDEMEREVMHRLQGRRGTL"
     gene            complement(5136..>5892)
                     /locus_tag="VFR00_14020"
     CDS             complement(5136..>5892)
                     /locus_tag="VFR00_14020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0160433.1"
                     /translation="AQRLSTLHPGFAFFAGLPAGVGLPSAEFDAWLLIGGGQLLWDEL
                     ARPFGLKPLVVGHGGEPVGLWSNVRLEQATDLAGLPVAADGLAARVLERLGARPIAVS
                     PPRLRAALAQGEVAAAHWAGPLPTPAFDLGPLAQCLYQPGLYANAALTTLTVERGLWE
                     QLAPADQAVFEACAAQECRLQLAEAKLYAGVAAAARTRSKWPLRRPLPAALTEALAAA
                     GSAVMAELAEHDVACRRIADSYAAYRDWSGADA"
BASE COUNT         1037 a         1924 c         1946 g          985 t
ORIGIN      
        1 caatgtcatc aacgccaaca gctttgagta tgctttcatt gagattgaac tcaaggacgc
       61 gcggccatga taggcagccg tggcggcagg ctgggcacgg ccggggcggc acggcaatcg
      121 acgcattggc gcgcgctttc aacacgcgtc ctgtaaagcg gcgcactgag gcacggtgac
      181 atgacaacgg cggctaagat catcctcgag ctcgacggcg agcctgtcga agccgtgcca
      241 ggcgagacca tctggcaact cgcccagcgt cacggctgca aaatcccaca cctgtgctgg
      301 cacccctccc cggggtatcg cgccgacggc aattgccgcg cctgcatggt ggaaatcgaa
      361 ggtgagcgcg tgctggccgc ctcctgtcag cgcaaggcgg ccgccggcat gaaggtgaag
      421 accagttccg aacgcgcgca gaaggcgcgc cagatggtgt tcgagctgct gctggccgat
      481 cagcccgaac ggggggatgg tcatgatcct gcctccaagt tctggggctg ggtggatgcc
      541 atgggcatcg gcaccaaagg gcgtttcccc gcgggcgagc ggccgccgag cgacgtcagc
      601 catgccgcca tcgccgtcaa cctcgacgcc tgtatcaact gcggcttgtg cgtgcgcgcc
      661 tgccgcgagg tgcagatgaa tgatgtgatc ggcatggcct atcgcgggca cggcgccaag
      721 gtcgtgttcg atttcgacaa ggccatgggg cattcgacct gcgtcgcttg cggggaatgc
      781 gtgcaggtct gcccgacggg cgcgctgatg gagcggacac tcctcgatgc caagggcaag
      841 cgcgtcaatt acgaaacgcg ctcggtcgat acgctctgtc cgtattgcgg ggtgggttgc
      901 cagacccagg tccatgtcaa ggatgataaa atcctctacg tcgacggccg caacggaccc
      961 gccaatgaaa atcgcctgtg cgtgaagggc cgcttcggct tcgactacat tcaccatccc
     1021 gaccggctga aacggccgct tctgcgtcgc gacggcattg gcaaaagtgc cgatttgcag
     1081 atccgccgcg acgaggtgga caaatacttt cgcgaagtca gctgggaaga ggcgatggcg
     1141 cacgccgccg ccgggctcga gcgcatcaag cgccgcgacg ggggcacggc catcgccggc
     1201 ttcggctcag cgaaaggctc caacgaggag gcctacctgt tccagaagct gatccggcag
     1261 ggcttcggca ccaacaacgt ggaccattgc acgcgccttt gccacgcctc ctcggtggcg
     1321 gcgctgatgg agggcgtgaa ctcgggtgcg gtcaccgccc cgtttacggc ggcaaaggac
     1381 agcgactgca tcatcgtgat tggcgcacgc ccggcggaga accaccccgt ggcggccacc
     1441 ttcctgaaaa acgcggccaa gcgcggcgcc aaattgatcg tgatggaccc acgcgggcag
     1501 agccagggtt tggcgcgcta tgcctcgcac acgctgcaat tcaagcccgg ccgcgacgtg
     1561 gcgctgctca atgccatgct caacgtcatt attgccgagg gcatggtcga catccagtac
     1621 gtgcaggctc acaccgaagg cttcgatagt ctgaaggcga aggtggaggc attcaccccg
     1681 gagaagatgg aagcggtgtg cggcatccct gccgcgacca tccgcgacgt ggcgcgactt
     1741 tatgcgcgct cagagcggtc gatcatcttc tggggcatgg gtatcagtca gcatacgcac
     1801 ggcaccgaca acgcgcgttg cctcattgcg ctcgcgctcg tgacgggtca gctcggccgc
     1861 ccgggcacgg ggctgcaccc gttgcgcggc cagaacaatg tgcagggcgc ctccgatgcg
     1921 ggtctgatcc cgatggttta tccagactac aggtcggtcg aggatcccct ggtcagaaac
     1981 gcttacgaga ccttctgggg tacggcgctc gaccccaaga agggattgac cgtggtcgag
     2041 atcatgcatg ccatcaagga cggcagcatc aaaggcatgt atatcctcgg cgaaaatccc
     2101 gcgatgtcgg atcccgacgt ccagcacgca cgcgaggcgc tggccaagct cgagcatctt
     2161 gtcgtgcagg acttgttttt gaccgagacg agctggcatg ccgacgtgat cctgcccgcc
     2221 tcggcgcacg cggaaaaatg gggcacgttc accaacacca atcggcaggt ccagattgcc
     2281 cgccccgtgc tcaacccacc tggagaagcg cgccaggatt ggctgctcat ccaggagttg
     2341 gcccagcgca ttggcctgcc ctggaattac cgcgacattt cggaagtcta tacggaaatg
     2401 gccgaggtga tgccctcgct caacaatatc agctgggagc ggttgctgcg cgaggatgcc
     2461 gtcacctacc cctgcgacgc cccggacaag ccgggcaacg aaatcatctt tgccgcctcg
     2521 tttccgacca aatcgggtcg cgccaagatc gtcccggccg atcttttgcc tcccgacgag
     2581 ctccccgatg cggactatcc cctggtgctg accaccgggc gactcctgga gcactggcac
     2641 acgggctcca tgacccggcg cgcgtccaac ctcgatagct tggagccgga agccatcgcc
     2701 ggcctgaacc cccgggagat ggagcggctc ggtgtttctg ccggtggatg ggtgcgggtg
     2761 tccacccgcc gcggcacggt ggtgctgaag gcgcgcgcgg accgcgacgt ggccgaaggc
     2821 atggtcttcc tgccgttctg ttttgcggaa gcggccgcca acctgctcac caatccgcag
     2881 ctggacccga tgggcaagat cccggaattc aagttctgcg cggccaaggt ggaaccggcc
     2941 gccgcggcga tggccgccga ataggccccc cgcgcttgca ggcgagggct ctagcgtgct
     3001 ggcggaagcc cgcgcccgag gcgccagcgc tcttcctcga cgcggatgtt ggccagcatg
     3061 cggtcgagca gcaggcaggt ccggcgataa aggtcggagc cacggcggta gtcggcaggc
     3121 gcaaaaaaat cgcagcggcc cgcgttgaag aggtccacgc atttggcctt tgacttgccg
     3181 ggcggataga cggcaacggc gtggccgccg ttcttgcgca tgaccgccat cgatggcacg
     3241 tcggtgtcgc cgtcgccgaa gtagatcatg ttggcaaagg ggatcggccg aagggcctcg
     3301 ggcatatgct ggttgatgct gcggcccaaa tcctcgacac ccttgttgat gcgaaacagg
     3361 tactgcgtct tgccggtgtc ggtgatcaca cgcttgggat agggcagatc gtaggcgtca
     3421 aaccagtact ccgaggcgaa cacgttatga aagcgcttgt agatgctggt gccctcgacg
     3481 atctcgctga gccccgacga aatgaggtag tgacgcagct gcacaccgtg gctctgggca
     3541 tggctcttga cgtagacgcc gatgctctca aaccagctct caacaccggc aaagagctcg
     3601 acctggcggc cctgggcgac gagatcgcgg cgatcgatgc gcacgccctt atctttggcc
     3661 ttcttgtaca tgagatgcat gtaagtgatc agcggatcgg cgtgcgcctg ggtagcgagc
     3721 gcattgctct cggcccagaa cgcggcggga tcagcgccaa tcttcggcag aaaggcatac
     3781 tcttgcatcg gccgcggcga cagcgtgccg tcaaagtcat agaccagcgc gatcgtcttt
     3841 ttatcgaatt tcggcaccgg tcgcgccgtg gcgctctcgg ggccgctggc gggctcgacc
     3901 tcaggggttt tgcgccggga acttgcgcga tcgaccatgg cttcgggctt ccgtgcgtga
     3961 gcgcacgtac cttcccccga ttcgcgtggc cccttgaagg cgcaggcgcg ggtcgtccac
     4021 tgacctggta tgacctggcg ccgctctgcg aggcaaagcc tgccatcccg cgctgcgcgc
     4081 gcgggcgtcg cccgggccat ctggtcgcag gggcggtgag cccttagcga aagcgccgct
     4141 gcgggggacg gcgcggcggg ccgctcgtca cgggcgcgcg cacatccttg tgccggaagt
     4201 tgatgcgacc cttgtcgaga tcgtagggcg tcatttccac agtcacgcgg tcgccggcca
     4261 ggatgcggat gcggttcttt ttcatgcgtc ccgaggtata ggccacgact tcgtgtccat
     4321 tatccaactt cacgcgacag cgcgcgtccg gcaagacctc ggcgaccaca ccttcgaact
     4381 ccagcatctc ttccttggcc atgcagtctc ctcgtttgcg gcccctagcg cagcagctcc
     4441 gagcgaggca cccgagcccc gactgcgggc gagcgcttgg cggctcgcca aggcggcgcg
     4501 ggcagcaggt tccctcgcgg atttgctgcc cgtatatggg ccggctggga agcgcgggca
     4561 agcggaaaac gcgcgggccc tgcacgcgca agcgcagcgc tcacgcacgc gtgagccctg
     4621 cgccagccgc tggctgcgag gaggagctgc gcgcggggcg gccaagcgcg acccgatcgc
     4681 agaccacttt agagcgtgcc gcggcggccc tgcagccgat gcatgacctc gcgctccatc
     4741 tcgtcggtga agaggccgcc gtggctatcg agataggcgg caatctcgcg ttcggcgcgc
     4801 cgctgctggg agcgcatgat ggcagcatag acgcgccgcc acagcgcgcc gcggaccggc
     4861 gcgcgcgcgc cggcgcccgg cgcatagtcg gcttccgtgt aggtcagggt gctcatggat
     4921 cagctccttt tcgttgcgcc aaccctgtgc aatttccccc tagcgtgctg cggggcgttg
     4981 gcgcgacaac gcgcgccggc ccttctggct ctttgccata tgggcgctgc ttgcaactaa
     5041 agtaggatcg atgttgcgct gcagcgatgc agtctctgca taggtagcgc ttgcccggat
     5101 cggtcggatt tccgcgggcg ggtgaggcac ttggcctagg cgtcagcccc gctccagtcg
     5161 cgataggctg cgtagctgtc ggcgatccgc cggcaggcga catcatgctc ggcgagctct
     5221 gccatgacgg cgctaccagc ggcggccagg gcctcggtca gcgcggccgg caggggcctg
     5281 cgcagcggcc atttgctccg cgtgcgcgcc gcggcggcca cgcccgcgta caatttggcc
     5341 tccgccagct gcaggcgaca ctcctgggcg gcgcaggctt cgaacacggc ttgatccgcc
     5401 ggggccaatt gctcccacag gccccgctcg acggtcaacg tcgtaagggc ggcgttggca
     5461 taaagcccag gttgataaag gcattgcgcc agagggccga gatcgaaagc cggcgtcggc
     5521 aacggtcctg cccagtgggc ggccgcgacc tcgccctggg caagggccgc ccgcagccgc
     5581 ggtggcgaca ccgcgatcgg ccttgccccc aggcgctcga gcacgcgcgc ggccaagccg
     5641 tcggccgcca ccggcaagcc ggcaagatcg gtcgcctgct ccaggcgtac gttggaccat
     5701 aggccgacag gctccccgcc gtggccgacc accagcggct tcaggccgaa gggccgcgcc
     5761 agctcatccc acaacagttg ccccccgccg atgagcaacc aagcgtcaaa ctcggcgctc
     5821 ggcaaaccca cgcccgctgg cagccccgcg aagaaggcaa atcccggatg cagggtgctc
     5881 agccgctgcg ca
//
LOCUS       DASTMT010000610         3036 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_5156185, whole genome shotgun sequence.
ACCESSION   DASTMT010000610 DASTMT010000000
VERSION     DASTMT010000610.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3036
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5156185"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            161..1327
                     /locus_tag="VFR00_14025"
     CDS             161..1327
                     /locus_tag="VFR00_14025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002714219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEU0160434.1"
                     /translation="MPTYARQNVVFERGEGAWLISTSGERYLDFASGVAVNALGHAHP
                     KLIAALTEQAHKLWHSSNLYRIAGQERLAERLCAATFADKVFFCNSGAEACEGAIKLA
                     RRYHFANGAPERWRIVTFKGAFHGRTLATIAAAGNEKHLEGFGYPADGFDLIALGDLG
                     AVETAIGPHTAGIMIEPVQGEGGARVVDPKFLQALRQVADKHGLLLILDEVQSGTGRT
                     GKLFAHEWAGITPDVMAIAKGIGGGFPVGAFLATNEAAKGMVPGTHGSTFGGNPLAMA
                     VGNAVLDAVLEPGFLQHVETVSLRFKQELARVKDEFPEVVEDVRGSGLLTGIKVKPPY
                     GDVVNACTAEKLLTVGAGDNVVRLLPPLNVTEAEITEATRRLAAALRRLAKPAA"
     gene            1355..2287
                     /gene="argF"
                     /locus_tag="VFR00_14030"
     CDS             1355..2287
                     /gene="argF"
                     /locus_tag="VFR00_14030"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEU0160435.1"
                     /translation="MSMAPRHFLDIDRIDAGTLRRIVDMGHAMKKAGKRVPAKLRPAG
                     IADAVLMLIFEKPSTRTRVSFDVAMRQLGGQTIALNHTDLQVGRGEPISDTAKVISRY
                     VDAIMIRANKHDTLNEFAEHATVPVINGLTDRTHPCQIIADIMTFEEARGPIRGRKVA
                     WTGDGNNVAASWIQAAARFDFRLALACPPQLNPERSVLEWARQHGGDVHVTEEPQEAV
                     AGADCVVTDTWVSMGQQNEASRRKQLLGPYAVDEALLRQANKDAIFMHCLPAYRGLEV
                     AASVIDGAQSVVLEEAENRLHAQKAILAWCLGVD"
     gene            2314..>3036
                     /locus_tag="VFR00_14035"
     CDS             2314..>3036
                     /locus_tag="VFR00_14035"
                     /inference="COORDINATES: protein motif:HMM:NF013589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HEU0160436.1"
                     /translation="MLPADDLVLPFRTVKSDVVGRVVRLGTAVDTVLSGHNYPEPVSY
                     ALGEALVLTAMLGSALKSEAKFILQTKTDGALDFLVADYVSPGKVRGYANYDKTDAAL
                     AKAQGRGDQGALLGSGHVAMTIDPTGDKNTYQGIVAIEHEPLVQVAHTYFRQSEQLPT
                     FIRLAVARHYGPAGPDLPPGWRWRAGGLLIQKLPRAGGKPSEVSAEERDATLEGEDDE
                     DWTRTRHLAATVEDHEMLDPTLS"
BASE COUNT          556 a          985 c          976 g          519 t
ORIGIN      
        1 gaaccttcgc cggatcgtca aacagcgccg cgcctaagcg cggccttttt cgttttctgt
       61 cacgagatag gtctctgtca tgtcaagcac cgcccaacag tccagcaagc ccagcgccag
      121 caaaccagcc gccgcgagcg catccccctc cgtggcggtg atgccgacct acgcccgcca
      181 gaacgtcgtg ttcgagcgcg gcgaaggggc ctggctgatt tccaccagcg gcgagcgcta
      241 tttggatttt gccagcggcg tggctgtgaa cgccttgggt cacgcccatc ccaagctcat
      301 tgccgcgctg accgagcagg cccacaagct ctggcactcg tccaatctct atcgcatcgc
      361 gggtcaggaa cggcttgccg aacgcctgtg cgcggcaacc tttgccgaca aggtgttctt
      421 ctgcaactcc ggggcggaag cctgcgaggg tgccatcaag ctcgctcgcc gctatcactt
      481 cgccaatggt gcgccggagc gctggcggat cgtcaccttc aagggtgcct ttcacgggcg
      541 cacgttggcc actattgcgg ccgccggcaa cgaaaagcat ctggaaggtt tcggctaccc
      601 ggctgacggc ttcgatctca ttgcgctcgg cgaccttggc gcggttgaga cggcgatcgg
      661 gccgcacacg gcaggcatca tgatcgagcc ggtgcaaggc gaaggcggcg cgcgcgtcgt
      721 cgaccccaag tttttgcagg cgttgcggca agtggccgat aagcacggcc tgttgctgat
      781 cctcgatgag gtgcagagcg gcacgggccg caccggcaaa ctctttgccc acgaatgggc
      841 cggcatcacg cccgacgtca tggccatcgc caaaggcatc ggcggcggtt ttccggtcgg
      901 ggccttcttg gcgaccaacg aggccgccaa gggcatggtg cccgggacgc atggctcaac
      961 cttcggcggc aacccgctgg cgatggcggt cggcaatgcg gtgctcgatg ccgtcctcga
     1021 accgggtttc ctgcaacacg tcgagacggt gtcgctgcgc ttcaaacagg agctcgcccg
     1081 cgtcaaggac gagtttccgg aagtcgtcga ggacgtgcgc ggcagcggac tacttaccgg
     1141 catcaaggtc aagccgccct atggcgacgt ggtcaacgcc tgcacggcgg aaaagctgtt
     1201 gacggtcggc gccggcgaca atgtcgttcg cctcctgccg ccgctcaacg tcacggaggc
     1261 tgagattacg gaagccacgc ggcgcctcgc cgccgcactg cggcgcctgg ccaagccagc
     1321 ggcttaagcc ggaccgtccc tgagagacgc cgccatgtcg atggcacctc ggcactttct
     1381 ggatatcgat cgtatcgatg ccggcacgct gaggcgcatc gtcgacatgg ggcacgccat
     1441 gaagaaggcg ggcaagcgcg tgccggcgaa actcaggccc gccggcattg ccgatgccgt
     1501 gctgatgctg attttcgaga agccgtccac gcgcacgcgc gtttccttcg acgttgccat
     1561 gcgtcagctt ggcggccaaa cgatcgcgct caaccacacg gacttgcagg tcggccgcgg
     1621 cgagcccata tccgatacgg ccaaggtcat ttcgcgctac gtcgatgcga ttatgattcg
     1681 cgccaacaag cacgacacac tgaacgaatt tgccgagcac gcgacggtac ccgtgatcaa
     1741 tggcctgacc gatcgcacgc atccctgtca gatcatcgcc gacattatga cgttcgaaga
     1801 agcgcgagga ccaatcagag gccgcaaggt ggcctggacc ggcgacggta acaatgtcgc
     1861 cgcgtcctgg atccaggcgg cggcgcgttt cgacttcagg ctcgcgctcg cctgccctcc
     1921 ccaactcaac cccgaaagat cggtgctcga atgggcacgg cagcacggcg gcgacgtgca
     1981 cgtgacggag gagccgcagg aggccgtggc cggtgcggat tgcgtggtca cggacacttg
     2041 ggtgtcgatg ggccagcaaa acgaggcgag ccggcgcaag cagctgctcg ggccctatgc
     2101 cgtcgatgaa gccctgctgc gccaggccaa taaggacgca atcttcatgc attgcctgcc
     2161 ggcctatcgc gggctcgagg tggccgccag cgtgatcgac ggcgcccagt cggtggtgtt
     2221 ggaagaggcc gagaaccgcc tgcacgcgca gaaggcgatc ctggcctggt gtcttggggt
     2281 cgattgatgc ccgaacccgg cagaacacca tccatgttgc cggccgacga tctcgtgctg
     2341 ccatttcgca cggtcaaatc cgacgtggtc ggacgcgtgg tgcgtctcgg cacggccgtc
     2401 gacaccgtgt tgtcgggcca caactatccc gagcccgtca gctatgccct gggtgaggcg
     2461 ctggtcctca cggccatgct cggcagcgcg ctcaagagcg aggccaaatt cattctgcaa
     2521 accaaaaccg atggcgcgct cgattttctg gtggccgatt acgtttcgcc gggcaaggtg
     2581 cgtggctacg cgaattacga caagacggac gccgcgctcg ccaaggcgca aggtcgcggc
     2641 gaccagggcg ccctgttggg ctcgggtcat gtcgccatga cgatcgaccc gaccggcgac
     2701 aagaacacct atcagggtat cgtcgccatc gagcacgaac cgttggtgca ggtggcgcac
     2761 acctattttc gtcagtccga gcaactgccc accttcatca ggttggccgt tgcccgccac
     2821 tatggtcccg cgggaccaga cttgccgccg ggctggcgct ggcgcgccgg cggactattg
     2881 atccagaagc tgccgcgtgc cggcggcaag ccgagcgagg tgagtgcgga agagcgcgat
     2941 gccaccctcg aaggcgagga tgacgaagat tggacgcgta cgcgccatct tgccgcgacc
     3001 gttgaagacc atgaaatgct cgacccgacg ctgtcg
//
LOCUS       DASTMT010000611         1576 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_6141592, whole genome shotgun sequence.
ACCESSION   DASTMT010000611 DASTMT010000000
VERSION     DASTMT010000611.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 1576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1576
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6141592"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..629)
                     /locus_tag="VFR00_14040"
     CDS             complement(<1..629)
                     /locus_tag="VFR00_14040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HEU0160437.1"
                     /translation="MRRSERPSNLRAAAMASGPAAAAAVKRVSVLGATGSVGQSTLDL
                     IGRNPNIFKVVALTAKHNAERLAELAVRHGAELAVIAEDAHYQDLRQRLAGTGIEVAA
                     GMAGLIEAALRPADCVMAGIMGAAGLRPTLAAVSQGKRVALANKECLVSAGETFMEAV
                     RRAGTELIPVDSEHSAAFQAIAGAAPDSIERIVLTASGGPFRTWSLEQLA"
     gene            complement(650..1522)
                     /locus_tag="VFR00_14045"
     CDS             complement(650..1522)
                     /locus_tag="VFR00_14045"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEU0160438.1"
                     /translation="MNETSEPGDQQGSPRTGLAWLGSDFPTRLVSGIAMAVIAAGALA
                     AGAAAFNALVFVLALVLSWEWGRLVHGRGAEIVTAVHVGAAALAVGLAALGFVGLGLL
                     ALPIGAILAMLLSLGRHSLFAALGVFYAGLPAVTLIWLRSDPTYGLRAAVFVILVVIA
                     ADTGAFLAGRGLAGPKLWPSVSPNKTWAGLLGGVLAGGIAGALMAMAVPGNPGLRLGA
                     TAAVLAFLAQMGDLMESAIKRRFGAKDTSALIPGHGGIMDRLDGLVIAATAAGLTGFF
                     VDVHSPARALLLGY"
BASE COUNT          286 a          589 c          482 g          219 t
ORIGIN      
        1 gcaagctgct ccaggctcca ggtgcgaaac ggcccgccgg aggccgtcag cacgatgcgc
       61 tcgatgctgt cgggggcggc gccggcgatc gcctggaagg cggccgagtg ctccgaatcg
      121 acggggatca gctcggtacc ggcccggcgt accgcctcca taaaggtctc acccgccgac
      181 accaaacact ccttgttggc gagcgccacg cgcttgccct gggacacggc cgccagcgtc
      241 gggcgcaagc cggccgcccc catgatgcct gccatgacgc agtcggcggg cctgagcgcc
      301 gcctcgatca gcccggccat gccggccgcc acctcgatgc cggttccggc caggcgttgc
      361 ctgaggtcct ggtaatgggc gtcttccgcg atcacggcca gctcggcgcc gtgcctgacg
      421 gcgagttccg ccaggcgctc cgcattgtgc tttgccgtga gcgctacgac tttgaagata
      481 ttgggatttc ggccgattaa atcgagcgtg ctctgtccca ccgaaccggt ggcgccgagc
      541 acgctcacgc gtttgacggc agccgcggct gccggccccg atgccatcgc cgccgcgcgc
      601 aggtttgacg gtcgctcact ccttcgcacc gccgctcttg cgcccattct caatacccca
      661 acagcagcgc gcgtgccggt gaatgcacgt cgacaaaaaa gccggtcaag cccgccgccg
      721 tcgccgcgat caccagcccg tccaagcgat ccataatgcc accgtggcca gggatcagcg
      781 cgctggtatc cttggcgccg aaccgacgtt tgatcgccga ttccataaga tcgcccattt
      841 gcgccagaaa ggcgaggacc gccgccgtgg ctcccagccg cagcccggga ttgcccggga
      901 cggccatggc catcagggcg cccgctatac cacccgccag tacgccacca agcagacctg
      961 cccatgtctt gttgggcgaa acgctcggcc acagctttgg acccgccagg ccgcgacccg
     1021 ccagaaatgc acctgtgtcc gccgcgatca cgaccagaat gacgaacacg gccgccctaa
     1081 gcccataggt cggatcggac cgcaaccata tcagggtaac cgccggtaag ccggcataaa
     1141 acaccccaag cgcggcaaaa aggctatggc gaccaaggct caacagcatg gccaaaatgg
     1201 ccccgatcgg cagcgccagc agaccaagcc cgacaaagcc cagggccgcc agtcccaccg
     1261 ccagggccgc ggcgccgaca tgaacggcgg tcacgatttc ggcgccacgg ccgtgcacca
     1321 ggcgccccca ttcccagctc aagaccagcg ccaagacaaa gaccagggcg ttaaaggcgg
     1381 ccgcgcccgc cgccaaggcg ccggcggcga tcaccgccat ggcgatgccc gagacaagtc
     1441 gggtgggaaa gtcgctgccc agccacgcca ggcccgtgcg gggagaaccc tgctggtcgc
     1501 ccggctcgct tgtctcgttc atcggcgcga ccccttgccc tgcccctcgg cgctagccgg
     1561 tcgattgcgt cgccac
//
LOCUS       DASTMT010000612         8456 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_4829046, whole genome shotgun sequence.
ACCESSION   DASTMT010000612 DASTMT010000000
VERSION     DASTMT010000612.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 8456)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8456)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8456
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4829046"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..797
                     /locus_tag="VFR00_14050"
     CDS             <1..797
                     /locus_tag="VFR00_14050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009026075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU0160439.1"
                     /translation="VAIIGAGATGTELAAELHRTMRQLVAFGLDRIDPDKHIKIHLIE
                     GGQRILPALPERLSSAAQKLLEGLQVKIHTSAMVAQVLPNGVRLASGQVIAAELVVWA
                     AGVKAPDVLKDLAGLESNRLNQLVVLPTLETTRDPNIFALGDCAACPWPGHQQPVPPR
                     AQAAHQQATHMLKQMKRRLAGKPLLAWRYRDFGSLVALGHYSTVGNLMGRLIGRSLFI
                     EGYIARLMYRSLYKMHQLALHGVTKVSLDTLARVITRRTEPHVKLH"
     gene            complement(858..1622)
                     /locus_tag="VFR00_14055"
     CDS             complement(858..1622)
                     /locus_tag="VFR00_14055"
                     /inference="COORDINATES: protein motif:HMM:NF022515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2865 domain-containing protein"
                     /protein_id="HEU0160440.1"
                     /translation="MRQIANVCENFPVPMQRLVLALLVAAGLVGLGMLFARLGGGLSA
                     QPVNPANLVHPVFSPQLVPVADWFEDIFRGLRRDPGPREEWDRGWERRRDRPLSPFED
                     EERERRTANGGYFRTMCVRLCDGFPIPMSFSTTRSQFARDARRCAQTCPAGRLFVYRN
                     PGGEMEDMVDLDGRPYHQLPTAFLHQSTYVENCTCHGNPWDPEALARHQAYAAQSAQQ
                     GAADAGRKLKPPQRRDSRWSRSERWVQNNQDEPERR"
     gene            complement(1734..2105)
                     /locus_tag="VFR00_14060"
     CDS             complement(1734..2105)
                     /locus_tag="VFR00_14060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HEU0160441.1"
                     /translation="MMGGSLARLTFLLFILGFGAVSHAETGAPAPDQDAAAVHTEMLG
                     APVFAADGVLIGQVSDISYDEEDQPKRIRMTSASALGLGTRMLEIPAGLFTILRGAVV
                     VDLPAEAVQALPELVEQEDER"
     gene            2720..2971
                     /locus_tag="VFR00_14065"
     CDS             2720..2971
                     /locus_tag="VFR00_14065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0160442.1"
                     /translation="MRNTMLAALVLGGALLASGQAAAYFDDSHYWGPPVYPVPYAYGY
                     SYARARCGHYWARPWIVNPCGGGRRVRHSHRHKKPMIDK"
     gene            complement(3008..3376)
                     /locus_tag="VFR00_14070"
     CDS             complement(3008..3376)
                     /locus_tag="VFR00_14070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018322545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3175 domain-containing protein"
                     /protein_id="HEU0160443.1"
                     /translation="MSATADDGEVPGVAVAKARRRKRAAAPKRAARAPRKWSAAVTRN
                     SDALDLERQVFAKATPEAIARSLKRSAERSRRRKASPYRSAMSMLTFYINRAGKNLPK
                     RRRDLLERSKVALRKAFGRQ"
     gene            complement(3373..3609)
                     /locus_tag="VFR00_14075"
     CDS             complement(3373..3609)
                     /locus_tag="VFR00_14075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011511742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0160444.1"
                     /translation="MARCDVCGNDYDKAFRVTQGARTMTFDSFECAIHAMAPHCAHCD
                     CRVVGHGIEAGGKVYCCAHCAKHEGVKGVKDRTA"
     gene            4012..4938
                     /locus_tag="VFR00_14080"
     CDS             4012..4938
                     /locus_tag="VFR00_14080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0160445.1"
                     /translation="MTHVGPAPAPSAASAASGSFVEWGAVLAGAFLAAAISFVLLTFG
                     TAIGLSATSPWPNSGVSAKLLATLAVLWAMMQQIGAFMAGGYVAGRMRARWHEPTQHE
                     VEFRDGLHGGLVWAVGIVIGAALAMATAGAAAKTGIDVASKAAGVVALSSSDPTDAVV
                     DTMLRPTTVAQATGAPTPPAAAAGTSPARARNADETRSEMSRVLASAVASGSLSDQNR
                     NYLAQVVAQRTGLSQQEAERRVNEAFTAAREAADKARKAAILTGFVTAASLIISFGAA
                     WWAAMRGGQHRDNAVPARFAFGRNRRPNVLSS"
     gene            5085..5513
                     /locus_tag="VFR00_14085"
     CDS             5085..5513
                     /locus_tag="VFR00_14085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013950226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HEU0160446.1"
                     /translation="MARGSKKLSSPLHLLHRAGQRADSLFAQHVGNHAFTPRQFTVLQ
                     AVANADGLSQTAIMLATGIDRSGTAELVRRLVSSGLLQRRRTKEDARVYAVRLTPQGR
                     KMLAIGERAARAAGVQLLASIPSRQRTAFIAALGSIIDDA"
     gene            5632..6402
                     /locus_tag="VFR00_14090"
     CDS             5632..6402
                     /locus_tag="VFR00_14090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0160447.1"
                     /translation="MLDLFDLLLLKRGLPTDDHTLSFRRIDGDPNAKVIYFLPWHTPL
                     SICRQAGFTPLNFLACYEMPCAIVSSDPSLGVKAMHLLVADAEALLLAHGVAPSEALI
                     VGLSVGTYPAIYLANRIGARVCAVAAADRGDLMLWQSPATRIIKRRAIQRGLRLWQFS
                     KAMLGYHPVQNLAGIRRDSVFVVGERDPFIPVRRRAGLLQALARFAPAAHIASVAGGH
                     VKTLMTSAHYQQAMLGIATPRKYWWIRLGGGPLQRAQA"
     gene            complement(6415..6798)
                     /locus_tag="VFR00_14095"
     CDS             complement(6415..6798)
                     /locus_tag="VFR00_14095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012591550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgcJ/EcaC family oxidoreductase"
                     /protein_id="HEU0160448.1"
                     /translation="MSEDERAIRDLVAKWMQASQAGDTETVLGLMTDDVVFLVPGQEP
                     FGKQAFAAASASMEGVRIEGTSEIRELNVLDSWAYLRGYLRVAMTPPGGNTVRRAGYT
                     LSIFHKQPNGRWLLARDANLLTPET"
     gene            complement(7063..8067)
                     /locus_tag="VFR00_14100"
     CDS             complement(7063..8067)
                     /locus_tag="VFR00_14100"
                     /inference="COORDINATES: protein motif:HMM:NF012976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0018576 - catechol 1,2-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0009712 - catechol-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dioxygenase"
                     /protein_id="HEU0160449.1"
                     /translation="MRAYTTETITDAVIEQMSTTPDPRLRQIMESAIRHLHAFAREVN
                     LTPAEWIKGIEFMTKVGQMCTPARQEFILLSDTVGLSALVNIMHDKTKVEEATSASLL
                     GPFFRENTPKFEPGEQIARNAEGAEVVLFGRVTSAKGEPLPGAQVTVWQTAADGRYDI
                     QTDASTMDCRGIFSTDAAGNYLLRTVRPLGYYIPLDGPVGGLVQAQKRHGMRPAHIHF
                     LISAPGYRELTTALYMAGDAHLEDDVVFGASGDLVAEIKTSDPACPMQGLPSVHFDFS
                     LSRESAADKLTGRVGADPGQITAGNVAAEKPYGSPPPGHAAETESKKGGILAGLFRGR
                     "
     gene            complement(8177..>8456)
                     /locus_tag="VFR00_14105"
     CDS             complement(8177..>8456)
                     /locus_tag="VFR00_14105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011654660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     ATP-binding protein"
                     /protein_id="HEU0160450.1"
                     /translation="LGRALMLAPRILLVDEPSVGLAPLLVARTIDAIKQLKEQHRLTV
                     LMAEQNFTQAIRIADRGYVIVHGKIAVEGRSAEELNNNELIKKFYLGL"
BASE COUNT         1377 a         2752 c         2798 g         1529 t
ORIGIN      
        1 aggtcgccat catcggcgcc ggtgccacgg gtacggagct cgccgccgag ctgcatcgta
       61 ccatgcgcca attggtggcc ttcgggctcg atcggatcga tcccgacaag cacatcaaaa
      121 tccatctcat cgaagggggc caacgcattc tgccggccct gccggagcgc ctctcgagtg
      181 ccgcgcaaaa gctgcttgaa ggcttgcagg ttaagatcca cacctccgcg atggtcgccc
      241 aggtgctgcc caacggggtt cggctcgcca gcgggcaggt cattgccgcc gagctggtcg
      301 tgtgggcggc gggcgtcaag gcacccgacg tgctcaagga tctggcgggc ctggagagca
      361 accggctgaa ccagctcgtg gtcttgccga cgctcgagac cacgcgcgat cccaacatct
      421 tcgcgctcgg cgattgtgcg gcctgcccct ggcccgggca tcaacaaccc gtgccaccgc
      481 gcgcgcaggc cgcccaccag caggcgacgc atatgctcaa gcaaatgaaa cgtcgcctgg
      541 ccggcaagcc gttgctggct tggcgctacc gcgatttcgg gtccttggtc gccttgggtc
      601 attactcgac cgtcggtaac ctgatgggtc ggctgatcgg ccgcagcctc ttcatcgaag
      661 gctacatcgc gcgcctgatg taccggtcgc tctacaaaat gcaccagctg gcgctgcatg
      721 gggtgacgaa agtatcgctc gatacgctcg cgcgcgtcat tacgcggcgc accgagccgc
      781 acgtaaagct gcattaagcg ggcgccgcag ccaaaataga ggcgagcgta atcctcatcg
      841 cctcgcctcc gcatggctta tctgcgctcg ggctcgtcct gattgttctg gacccagcgc
      901 tcggagcggc tccaacggct gtcccgacgc tgtggcggct tcagcttgcg cccggcgtcg
      961 gcggcgccct gctgcgcgct ttgcgccgca taggcttggt gtcgggccag tgcctcgggg
     1021 tcccagggat tgccgtggca ggtacaattc tcgacgtagg tgctttggtg caaaaaggcg
     1081 gtcgggagct ggtgataggg gcgcccgtcg aggtcgacca tatcctccat ttcgccgccg
     1141 ggattgcgat agacgaagag gcggcccgcg gggcaggtct gcgcgcaccg gcgggcatcc
     1201 ctggcaaatt gagagcgcgt ggtgctgaaa ctcatcggga tgggaaaacc atcgcagagg
     1261 cgcacgcaca tggtgcggaa gtagccgccg ttggccgtgc ggcgctcgcg ctcctcatcc
     1321 tcgaaggggc tcaagggacg atcccgccgg cgctcccagc cgcggtccca ctcctcgcgc
     1381 ggccccggat cgcggcgcaa gccgcgaaaa atgtcctcga accagtcggc gaccggcacg
     1441 agctgcgggg agaaaacggg atggaccaga ttggctgggt tgacgggttg cgcgctgagc
     1501 ccgccgccga ggcgggcgaa gagcatgcca agacccacca gacccgcggc aacaaggagt
     1561 gcaagaacga ggcgctgcat gggtacgggg aagttctcgc agacattcgc tatctggcgc
     1621 acaacgtcgg cgcggcgcgc cgagttccag ggctgcgaaa ggtcggaaag tggcaggccg
     1681 cggggatgac gccgaccgcg gccctcgctg gggccggcga acggcgggca ggcctagcgt
     1741 tcgtcctcct gctccaccag ttcgggcagg gcttgcacgg cttcggccgg caggtcgacg
     1801 acgacggcgc cacgcaggat ggtgaaaagc ccggctggaa tttccagcat gcgcgtgccc
     1861 aaccccaagg cggacgcgct tgtcatgcgg atccttttcg gctggtcctc ttcgtcatag
     1921 gagatgtcgc tgacctggcc gatcagcacg ccgtccgccg cgaagaccgg cgcgcccaac
     1981 atctccgtgt ggaccgcggc ggcgtcctga tccggggccg gggctccggt ttcggcgtgg
     2041 gagacggcgc cgaaccccaa aatgaacaga aggaacgtta atcttgccag cgacccaccc
     2101 atcatacgct ccgctcgcgg tcgttaaccg ttggccaaac ggccagtgca aaacttggtt
     2161 cctgagcctt gcacttgcgc gagccaatgg ggcgccagca aggcgtcgca aaagcgccag
     2221 ccttgccccc cgggaggggc cgaggcggcc agacattctg ctgggcaaac cccgggcaac
     2281 cctaggctcg ggccagggcc ctgacgccat tggcgcggcc gggagggacc gtgacggcag
     2341 acgcaaaact cccctggccg cccaaggccg atggacgccg ggggcggggc gcccccgcgg
     2401 gacggcatgc tcgcttggta aacttggggg tgcctccccg gacgccctcg ccggcgagac
     2461 ccccgggtgt gatcgcgcaa acggcggcgg gtcggcagcg cagccccgtg aaacccgggg
     2521 gtgttgaggg tgcgttgagg atgacacgcg ggcacttttt tcgcgcgcga gcgatcctga
     2581 tagcgtgcac ctgtgctagg gccaaaggcg ggtgtcacgc cattgacgtc ggggtgagat
     2641 cattaacatc cgtgtggcat catgagcgcc ccacacggat caacgtcccc gtccaatgcc
     2701 gtcagggaga atatctcgca tgcgcaatac gatgctcgcg gccttggtgc tcggcggtgc
     2761 cctgcttgcc agcggtcaag cagcagccta tttcgacgat agccattatt gggggccgcc
     2821 ggtttacccc gtcccctatg cttacggcta ctcctacgcc cgcgcgcgct gcggccacta
     2881 ttgggcgcgc ccctggattg tgaacccctg cggcggcgga cgtcgcgtgc gtcacagcca
     2941 tcgccacaaa aagccgatga tcgacaagta gggcggccga agcctgccgt gctgagctgc
     3001 ggcgatgtca ttggcgccca aatgccttgc gcagagcgac cttgctgcgc tcgagcaggt
     3061 cgcgtcggcg tttgggcagg tttttgccgg cccgattaat gtagaatgtc agcatcgaca
     3121 tggccgaccg gtagggcgat gccttgcggc gtcggctgcg ttccgcggag cgtttgagcg
     3181 aacgtgcgat cgcctcgggc gtagccttgg cgaagacctg gcgttcaagg tcgagcgcat
     3241 cgctgttgcg cgtgacggcg gccgaccatt tccgtggcgc acgcgcggcg cgcttgggcg
     3301 cggcggcccg ctttcgtctc cttgccttgg ctactgccac gcctgggacc tcgccatcat
     3361 cggccgttgc gctcaagctg tacgatcttt cacgcccttc acgccctcgt gcttggcgca
     3421 atgggcgcag caatagactt tgccgcccgc ctcgatgcca tgcccgacca cgcggcaatc
     3481 gcagtgcgca cagtgcggcg ccatggcgtg aattgcgcat tcgaagctgt cgaaggtcat
     3541 cgtccgcgcc ccctgcgtca cccgaaaggc cttgtcgtag tcgttaccgc agacatcgca
     3601 gcgagccatc gcacgctcct ctttcctcac aaagctctat cgggcattaa cccgcggtcg
     3661 tgctccgggt tcccgccccg gactcccggc tggcgcgcgc ctcgagctgg tcgttgccgt
     3721 ggcagcgatg cgggtcgtcc caatcaagcc gagcaaggct gcttacggct atcgcgccgg
     3781 tagagctcgg ctgcgcaatg agcggacaat tgacaggaat ttggtggcga cgcggaacat
     3841 cggcgcctgc caccagttat tgcgccagat tgttcgaacc cgcacgatcg tcttgggtct
     3901 atgcagcgac ggtgaccagt catgaacgat atcatctaca tcatcgggct catcgtggtt
     3961 gtgatgttca tcttgagcgt cctcgggctt cgctaacttg gaggcattgt catgacgcac
     4021 gtcggtccgg ccccagcccc aagcgcagcc tcggcggcga gcggtagctt cgtcgaatgg
     4081 ggcgccgtcc ttgccggcgc ctttctcgcc gcggcgatct cgttcgtgct tctcaccttt
     4141 ggcaccgcga tcggtttatc ggccacctca ccgtggccga attcgggtgt ctcggcaaaa
     4201 ctgttggcaa cgctcgccgt gctgtgggcg atgatgcagc aaattggggc cttcatggcc
     4261 ggcggctacg tggccgggcg catgcgggcg cgctggcacg agccgacgca gcatgaggtc
     4321 gagtttcgcg acggactgca tggcggcctg gtatgggccg tggggatcgt gattggcgcg
     4381 gcgctggcaa tggcgacggc gggcgcggcg gcaaagacgg gcatcgacgt ggcgagcaag
     4441 gctgccggcg tggttgcgct ctccagctcc gacccgaccg atgccgtcgt cgataccatg
     4501 ctgcgtccaa cgacggtggc gcaggcgacg ggcgcgccca cgcctccggc ggcagccgcg
     4561 ggaacctcac ctgcccgcgc ccgcaacgcc gatgagacgc gcagtgagat gtcacgcgta
     4621 ctggcgagcg cggttgcaag cggctcgttg tcggatcaga accgcaacta cctcgcgcaa
     4681 gtcgtggcac agcgcaccgg gctttcgcag caggaggccg aacggcgcgt taacgaggcc
     4741 ttcacggcgg cgcgcgaagc ggccgacaag gcgcgcaaag cggccatttt gaccggcttt
     4801 gtcaccgcgg ccagcctgat catttcgttc ggggcagcct ggtgggcggc catgcgcggt
     4861 gggcagcacc gtgacaacgc ggtaccggcc agattcgcct tcggtcggaa ccgtcgtccc
     4921 aatgtgctgt cgagctaacg gaggttgtca tcatgttgcg agatatcatc ctctggctcg
     4981 ccggcgtgcc gatcttcgtc attattctgc tgcacctatt cggcttcctg cattgagccg
     5041 agcactggta gatcgcggac ggcgcggcgc gggaagggac atcgatggcg cggggcagca
     5101 agaaactttc ctcacccctc cacttgttgc accgcgcggg gcagcgcgcc gattccctct
     5161 tcgcccagca cgtcggcaac cacgccttca cacctcgcca attcaccgtg ctgcaggccg
     5221 tggccaacgc ggatgggctc agccagacgg caatcatgct ggcgacgggg atcgatcgct
     5281 cgggcacggc cgagctggtg cgtcgcctcg tctccagcgg cttgctgcag cggcggcgga
     5341 ccaaagagga cgcgcgcgtt tatgccgtgc gtctcactcc gcaagggcgt aaaatgctgg
     5401 cgatcggcga gcgtgcggcg cgtgccgccg gcgtccagct gctggcctcg atcccgagcc
     5461 gacaacggac tgccttcatc gcggcgctgg gctccatcat cgacgacgct tgacgccgaa
     5521 agctgtcgtc ttccgcgagc gtttgtttcg ccgggcggga aaaattcaag gtacggagga
     5581 tggccaggct gacacggaac ccagctttgc gccgctggtt aatagttcgc tatgctggac
     5641 ctgttcgacc tcctgcttct gaagcgcggc ctgcctaccg atgatcacac actgagcttt
     5701 cgccgcatcg acggtgatcc caacgccaag gtgatttatt ttcttccctg gcacacgccg
     5761 ctgagcattt gtcgccaggc tggtttcaca ccgctcaatt ttctcgcctg ctacgagatg
     5821 ccgtgcgcca tcgtcagctc cgatccgagc ctcggggtca aggcgatgca cctgctcgtg
     5881 gccgacgccg aggccctgct tttggcgcac ggcgtcgcgc ccagtgaggc gctgatcgtg
     5941 gggctgagcg tcggcaccta tcccgccatc tatctggcca atcgcatcgg ggcccgcgtg
     6001 tgcgcggtcg cagcggccga tcgcggtgac ctcatgctct ggcaaagccc ggccacgcgc
     6061 attatcaagc gccgcgcgat ccagcgcggt ctgcgcttgt ggcaattctc caaggcgatg
     6121 ctgggctacc atcccgtaca gaacctggcc ggcatcaggc gcgacagtgt gtttgtggtg
     6181 ggtgagcgcg acccgttcat accggtccgt cggcgcgcgg gattgttgca ggccctcgcc
     6241 agatttgcgc ctgccgcgca cattgcaagc gtcgcgggcg ggcacgtcaa aaccttgatg
     6301 acgagcgcgc actatcagca ggccatgctc ggcatcgcca cgccgcgaaa atactggtgg
     6361 atccgccttg gcggcggccc cttgcagcgg gcccaggcct gagcagcttg cgcctcaagt
     6421 ctccggcgtc agcaggttgg catcgcgcgc caaaagccaa cgaccattcg gctgcttgtg
     6481 gaaaatcgac agcgtatagc cggcccggcg cacggtattg cccccgggcg gggtcatcgc
     6541 cacccgcaga tagccgcgca ggtaggccca gctgtccagg acgttcagct cgcgaatttc
     6601 gcttgtgccc tcgatgcgca cgccctccat gctcgccgag gcggccgcga aggcctgctt
     6661 gccgaagggt tcctggcccg ggaccaagaa cacgacatcg tcggtcatca gccccagcac
     6721 cgtttcggta tcgccggcct ggctcgcctg catccatttg gcgacgagat cacgaatggc
     6781 ccgttcatcc tcgctcatgg cgtcactccg accaagacgg ggtaagccgt cggttgttag
     6841 agccggctca aaagtcttgt tgctaggacg gccacggcag cggcgctttt cctcccagca
     6901 gggctgcttt ggcggggcgc ggcgagcccg gccacccccg agcaacgctt ctgccggcga
     6961 aacgttcgcg cctggacagc caagccgcgc cgtggcgcga gcgggcgcgg ccatccggcg
     7021 cggcggcaag gcccgttgcg gcaggtccgc gggcgctgcg gttcaacggc cgcggaacag
     7081 gccggcaagg atgccgccct tcttgctctc ggtttcggcc gcgtgcccgg gaggcgggct
     7141 gccatagggc ttttcggcgg cgacattgcc ggcggtaatt tgtccgggat cggcaccgac
     7201 gcgacccgtc agcttgtcgg cggcgctctc gcgcgacagg ctgaaatcaa aatgcacgct
     7261 cggcaggccc tgcatcggac aggcgggatc gctggttttg atttccgcca ccagatcgcc
     7321 ggaggcgccg aacacaacgt cgtcttccag gtgagcatcg ccggccatgt agagcgcggt
     7381 ggtcaattcc cgataacctg gcgcgctgat caggaagtgg atatgagctg ggcgcatgcc
     7441 gtgccgtttt tgcgcttgaa ccaagccgcc gaccggtccg tcgagcggga tgtagtaacc
     7501 gagtggtcga acggtgcgca ggagatagtt gcccgcagca tccgtgctga agatgccgcg
     7561 gcagtccatc gtgctggcgt cggtctggat gtcgtagcga ccatccgcag ccgtctgcca
     7621 cacggtgacc tgggcgcccg gcaggggctc gcccttggcg ctggtcacgc gaccgaacag
     7681 cacgacttcc gcgccttcgg cgttcctggc gatttgttcg ccgggctcga atttgggcgt
     7741 attctcccgg aagaagggtc caagcaggct ggcactcgtc gcctcctcga ccttggtctt
     7801 gtcgtgcatg atgttgacga gcgcggacag gccgaccgtg tccgacagca gaatgaactc
     7861 ctggcgcgcc ggggtgcaca tctgcccgac cttggtcatg aactcgatgc ccttgatcca
     7921 ctcggccggg gtcaggttga cctcgcgggc gaaggcgtgc aggtgtctga tcgcgctttc
     7981 catgatctgg cgcaagcgcg ggtcgggcgt ggtggacatc tgctcgatca cggcgtcggt
     8041 gatcgtctcc gtcgtatagg ctcgcatcgt gctctctccc ttcgcggcgg cgcacagggc
     8101 ctgcctctcg tggcccaagc tgttccaggc cgttcaaggt gtcaagttca ggcgccgtgc
     8161 ctcgccgtta cccccgtcac agtcccagat agaacttttt gatcagctcg ttgttgttga
     8221 gttcttcggc ggaacggcct tcgacggcga tcttgccgtg cacgatcacg tagccgcgat
     8281 cggcaatgcg aatggcctgg gtaaagttct gttcggccat cagcacggtc agccggtgtt
     8341 gttccttaag ttgcttgatg gcgtcgatgg tgcgggctac caacaagggg gccaggccga
     8401 cggagggttc gtcgacaaga aggatgcgcg gggccagcat gagcgcccgg ccgagg
//
LOCUS       DASTMT010000613         2841 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_5774888, whole genome shotgun sequence.
ACCESSION   DASTMT010000613 DASTMT010000000
VERSION     DASTMT010000613.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2841)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2841)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2841
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5774888"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..686
                     /locus_tag="VFR00_14110"
     CDS             <1..686
                     /locus_tag="VFR00_14110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEU0160451.1"
                     /translation="KMSKSVGNVVDPFDLVKGYGVDQVRYFFLREAAFGQDGSYSHEA
                     IVNRINADLANDLGNLAQRSLAMIAKNCAGTLPTPGDLSPADRAILAEADALYAKVRA
                     AMDRQGIKQYLDAVWAVVADANRYFAGEEPWAKRKTDPKRMETILYVTAEVVRQVAIL
                     AAPVMPGSAAKLLDSLGQDAGKRSFAWLGEAGRLAPGASLAPPVAVFPRYLDPGAEGT
                     AKPPKPQKP"
     gene            686..1480
                     /locus_tag="VFR00_14115"
     CDS             686..1480
                     /locus_tag="VFR00_14115"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013895615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEU0160452.1"
                     /translation="MLVDHHCHLDFPDFADELEAVLQRARAAGVGAVVSISTRLRRLA
                     KLMAIVEANANVFCSVGTHPHYAHEELDIPLEELVRLAAHPKVVAIGEAGLDYFYDNS
                     PRAAQEQGFRRHIAAARATQLPLVIHARDADADVAAILEAETAKGAFPAVLHCFTGGR
                     ELAERGLALGHYLSFSGIITFKKSDSLREVAALVPLDRLLVETDAPYLAPSPFRGKRN
                     EPAYVVHTAAALAAVKGISPQELARITTENFLRLYSKVPPTAIAQS"
     gene            1477..2274
                     /locus_tag="VFR00_14120"
     CDS             1477..2274
                     /locus_tag="VFR00_14120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011441570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU0160453.1"
                     /translation="MSLKITLLGTGSSGGVPRVGNIWGACDPSNPKNRRRRCAILVEK
                     ITPRGRTRVLVDTPADLRDQLLEAEIDCVDAVLYTHDHADHTHGIDDLRGMAFNARRR
                     IDIYADARTRQVLEQRFDYCFVQPPGSAYPPILNAHTIDPATALEVGGSGGVIKIVPL
                     PQAHGDLPSLGFRFGDFAYSPDISGIPDASLPLFANLDLWIVDALRPLPHPSHFSVKQ
                     ALRWIERLGVKRAILTHMTSELDYETLRRGLPAHVEPGYDGMIVTIG"
     gene            complement(2288..>2841)
                     /locus_tag="VFR00_14125"
     CDS             complement(2288..>2841)
                     /locus_tag="VFR00_14125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012332445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU0160454.1"
                     /translation="ATLLTLNLNDHDRIDALGPTIFQRWGKLDVLVGNAGVLGPLSPL
                     GHVTAEAWKEVIDLNLNTNWRLIRTLDPLLRRSEAGRAIFVTSGAALNRRAYWGPYSV
                     SKAALEALVQTYAAEMVNTNVRANLINPGPVRTAMRAKAFPGEDPATLPWPEDITERF
                     VELATAAASYNGRIFDLKTNEVR"
BASE COUNT          484 a          962 c          906 g          489 t
ORIGIN      
        1 agaagatgtc gaaatcggtg ggcaacgtgg tcgatccttt cgacctggtc aaaggctacg
       61 gtgtcgacca ggtgcgctac ttctttttgc gcgaggccgc cttcggccag gacggcagct
      121 acagccacga ggccatcgtc aaccgcatca acgccgacct cgccaatgac ctcggcaacc
      181 tggcgcagcg ttcgcttgcc atgatcgcca agaactgcgc gggcacgctc cccacccccg
      241 gcgacttgag ccccgcggac cgggcgatcc tcgcagaggc cgatgcactc tatgccaagg
      301 tgcgcgccgc catggaccgg cagggcatca agcaatacct ggatgccgtc tgggccgtgg
      361 tcgccgacgc caatcgctat ttcgcgggcg aggagccctg ggccaaacgc aagaccgatc
      421 ccaaacgcat ggagacgatc ctctacgtga cggccgaggt cgtgcgccag gtcgccattc
      481 tcgcggcacc ggtgatgccg ggctcggccg ccaagctgct cgacagcttg gggcaggacg
      541 cgggcaagcg cagctttgcc tggctgggcg aggcgggccg gctcgcgccg ggcgccagcc
      601 ttgccccgcc cgtcgccgtc tttccacgct atctcgatcc cggcgcggag ggcacggcca
      661 agccgcctaa accacagaaa ccctgatgct ggtcgatcat cactgtcacc tcgacttccc
      721 cgattttgcc gacgagctcg aggccgtgct gcagcgcgcg cgggcggccg gcgtcggcgc
      781 cgtcgtcagc atttcgacgc gcctgcggcg gctcgccaag ctgatggcga tcgtcgaggc
      841 caacgccaat gtcttctgct cggtgggcac gcatccgcat tacgcccatg aggagctgga
      901 tatcccgctc gaggagctgg tgcggttggc agcacatccc aaggtggtgg caatcggcga
      961 ggcggggctc gactattttt acgacaacag cccgcgcgcg gctcaggagc aaggatttcg
     1021 ccggcatatc gcggcggcgc gcgcaaccca actgccgctc gtgatccacg cccgcgacgc
     1081 ggatgccgac gtcgccgcaa tcctcgaggc cgaaacggcc aagggcgcct tcccagccgt
     1141 cttgcactgt tttaccggtg gcagggagct ggccgagcgc gggctggcgc tggggcatta
     1201 cctgtcattc tcgggtatta tcaccttcaa gaagtcagac agcctgcgcg aagtggcggc
     1261 ccttgtacct ctcgatcgcc tgttggtgga aaccgatgcg ccctacctgg cgcccagccc
     1321 gttccgcggc aagcgcaacg agcccgctta cgtcgtgcac acggccgccg cgctggccgc
     1381 cgtcaagggc atctcgccgc aggaattggc ccgcatcacc accgaaaact tccttcgcct
     1441 ctacagcaag gtgccgccca cggcgatagc ccaatcatga gcctcaagat cacgctcctt
     1501 ggcaccggat cttcgggtgg ggtgccgcgc gtcggcaaca tttggggcgc gtgcgatccg
     1561 agcaatccaa agaaccggcg gcggcgctgc gccatcctgg tggaaaagat caccccgcgc
     1621 ggtcgcacgc gcgtcctggt cgatactccc gcggatctgc gcgatcagct gctcgaggca
     1681 gagatcgatt gcgttgatgc ggtgctctat acccacgacc acgccgacca cacgcacggc
     1741 atcgacgatc tgcgcggcat ggccttcaac gcccgccggc gcatcgacat ctacgcggac
     1801 gcgcgcaccc gccaagtgct cgagcagcgc ttcgactatt gtttcgtgca gccgccgggc
     1861 tcggcctatc caccgatcct caacgcccac accatcgatc cggcgacggc gctcgaagtc
     1921 ggcggcagcg ggggcgtcat caaaatcgtg ccgcttcccc aggcacacgg cgatcttccg
     1981 tccctgggtt ttcgcttcgg tgactttgcc tattcgcccg atatcagcgg cattcccgac
     2041 gcctccttgc cgctgttcgc taacctcgac ttgtggatcg tggatgcctt gcggccgctg
     2101 ccgcacccca gccattttag cgtcaagcag gcgctgcggt ggatcgagcg cctgggcgtc
     2161 aagcgggcca tcctcaccca catgacctcg gaactcgact acgagacgct gcgccgcggc
     2221 ctgcccgcgc acgtggagcc gggttacgac ggcatgatcg ttaccatcgg ctaaagcgct
     2281 gaggcgtcta gcgcacctcg ttggtcttca gatcgaagat gcggccgttg taggacgcgg
     2341 cagccgtcgc cagttccacg aacctctcgg tgatgtcttc cggccagggc aaggtggcgg
     2401 gatcttcgcc cggaaaggcc ttggcgcgca tggccgtgcg caccgggccg ggattgatga
     2461 ggttggcacg cacgttggtg ttgaccatct cggcggcata ggtttgcacc agcgcctcga
     2521 gcgccgcctt ggagacgctg taggggcccc agtaggcgcg tctgttcaag gcagcacctg
     2581 aggtgacgaa aatggcgcgg ccggcctccg accggcgcag cagcggatcg agcgtgcgga
     2641 tcagacgcca gttggtgttg aggttgagat cgatcacttc cttccaagcc tcggccgtga
     2701 cgtggccgag cggcgaaagg ggtcccagca cgccggcatt gccgacgaga acgtcgagct
     2761 tgccccaacg ctgaaagatg gtggggccca gcgcgtcgat cctgtcgtga tcattgaggt
     2821 tgagggtcaa aagcgtggcc g
//
LOCUS       DASTMT010000614         5409 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_5486867, whole genome shotgun sequence.
ACCESSION   DASTMT010000614 DASTMT010000000
VERSION     DASTMT010000614.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 5409)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5409)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5409
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5486867"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..393
                     /locus_tag="VFR00_14130"
     CDS             <1..393
                     /locus_tag="VFR00_14130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3754 domain-containing protein"
                     /protein_id="HEU0160455.1"
                     /translation="FHAVADNRGVMTLLADRAAEEDVKEEMLLYSALAKAPVNVRDLK
                     AVDEAIEAYIKDTFDLDVDFDISDALERLKQDELVTELPDGTLRALPPHAAAKQIDKL
                     WDQCLDQLPDPPAIGHEFDGATGGPAAA"
     gene            528..2366
                     /locus_tag="VFR00_14135"
     CDS             528..2366
                     /locus_tag="VFR00_14135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011663550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase family protein P"
                     /protein_id="HEU0160456.1"
                     /translation="MFQSYEAPHAGGDHAARVRQLRQRLLALGLDALLVPRADEHQGE
                     YVAAAAERLAWLTGFTGSAGFAAIARSAAALFVDGRYIVQAPTQVDTNLFQILQVPQA
                     KLSQWLIDSVGSGGVVGYDPWLHAEAGIEDLGETLAGKGIKLKALAKNPVDQVWGRAR
                     PAPPQGPVVPQPLAYAGKGAQEKISELQASLRRDNADAVILTQPDSIAWLFNIRGADV
                     AHNPVPLAFAVVPASGKPELFIDKGKLGREARAHLAPLAKLSAPALLAARIAALRAAG
                     KRVRLDPHSAANWFFRKLRGGKGRIVRGVDPCVLPKARKNAAEIKGARAAQQRDGAVM
                     ARFLAWLEREGSSGTLDEIGVARQLENLRRETQALKEISFDTISGAGPNGAIVHYRVT
                     TATNRTLRAGELYLVDSGAQYLDGTTDITRTVALGSPTREMQERFTLVLKGHIAIATA
                     RFPAGTRGVDLDAFARRALWQAGLDFDHGTGHGVGSYLSVHEGPQNISKRGNTPLEPG
                     MIISNEPGYYKAGAYGIRIENLLLVEEPRAVPGGEREMLGFETLTLVPIDRRLIVPAL
                     LSDGERAWLDAYHARVRDKIGPELGPADLTWLTAATAPLPTRAKAP"
     gene            complement(2363..4261)
                     /locus_tag="VFR00_14140"
     CDS             complement(2363..4261)
                     /locus_tag="VFR00_14140"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEU0160457.1"
                     /translation="MPKQRTPFGRWPSPLSPRLAAAGSRRFGTLAAGAQALYWSQSRP
                     EQGGRQSIFAGSTDGGVREVLPAPFSARSRVHEYGGGEFLLADDTLYFVNDGDQQIYA
                     VAQGQAPKRLTEAAGMRFADLVLDRRRRRLIGIGERHAGATPKNMLLAVNLADGAVRE
                     LAGAHDFYASPCLAPDGERLAFLTWDLPDMPWDSARLSIAPVAADGSLGKAKSIAGGN
                     GAAAFQPTWSARTGWLYLVLDISGWGQLYAWDGSKLRRRLGRRGAELSRPQWVFGMRS
                     YCLSPEDRIAAVSLQQGTPLLEVVEPKRGRRTAYAALSRQAARIDDPVAWAGGFAALI
                     SRPTAMPEVMRIGPRGFRGFTAPGAREPALEALTGSISAGETLAFRAGHHRVYGIYYA
                     PANATHRGPAGALPPALLFVHGGPTSMTDAGLKLRVQFFTSRGFAVFDLNYAGSTGFG
                     RAYRERLDGTWGIADVADCVAGAKFLARTGRADARRIAISGGSAGGYTTLMALATSRA
                     FAAGSCHYGVSDLALLLKHTHKFESGYLHRLMGTTPKTWRAVFRARSPLELCDGITAP
                     VILFQGLEDKVVPPEQSRGMVARLKARGIDVAYHEFAGEAHGFRRAETIAAVLAAELA
                     FLERVLRL"
     gene            4691..>5409
                     /locus_tag="VFR00_14145"
     CDS             4691..>5409
                     /locus_tag="VFR00_14145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit alpha"
                     /protein_id="HEU0160458.1"
                     /translation="MTWERTDSAGHPDLAAFQDRQARGLFDPSREYDACGVGFIANLK
                     GGKTHKLIEDALCILENLEHRGAVGADPIAGDGAGVLIQIPHAFLEAEAQKLKFKLPK
                     PGHYACGHLFLPRDERLRAHCERVWTRIIREEGLEFIGWRSVPVDNSCLSEMVKATEP
                     VHRQIFISRPKAMKEQQEFERRLYMIRKVVSNALYYAYKGRDIGHYSVSLSSRTLVYK
                     GMFLSYQVKKYYKDLSDPRLAS"
BASE COUNT          888 a         1808 c         1898 g          815 t
ORIGIN      
        1 ttccacgcgg tggcggacaa tcgcggcgtc atgaccttgc tggcggatcg cgccgcggag
       61 gaagacgtca aggaggagat gctgctctat agcgcgctgg ccaaggcgcc cgtcaacgtg
      121 cgggacttga aagcggtcga cgaagcgatc gaggcctata tcaaggacac cttcgatctc
      181 gacgtcgatt tcgacatctc cgatgcgctg gaacggctca agcaagacga gctcgtcacg
      241 gagctcccgg acggcacgct gcgcgccttg cccccgcacg ccgccgccaa gcaaatcgac
      301 aagctgtggg atcaatgcct cgatcagctg cccgaccctc ccgccatcgg tcacgaattc
      361 gatggggcca cgggcggccc ggccgcggct tagcctggca ttttggcgcg gtctcgcgct
      421 cgcccgcgaa ctggggcagg attggcaccg catcggtcgc gcggaccgcc ggcgccgtgc
      481 tcttgaggtg ccgctggccg gcggccggct gaccgcaagg aggtgccgtg ttccaaagct
      541 atgaggctcc ccacgccggc ggtgatcacg ccgcgcgcgt gcgccagctg cgccaacgcc
      601 tgctcgcgct cggcctcgac gccctgttgg tgccgcgcgc cgacgaacat cagggcgagt
      661 acgtcgctgc cgcggccgag cggcttgcct ggctcacggg cttcacgggc tcggccgggt
      721 ttgccgccat cgcgcgctcg gcggcggccc tcttcgtcga cggccgctat atcgtgcagg
      781 cgccgaccca ggtcgatacc aacctgttcc agattctgca ggtcccgcag gccaagctgt
      841 cgcagtggct gatcgacagc gtcggcagcg gcggggtggt gggctacgac ccctggctgc
      901 acgccgaggc ggggatcgaa gacctgggcg agacgctcgc cggcaaaggc atcaagctga
      961 aggcgcttgc caaaaacccg gtggaccaag tttggggccg ggcgcgcccg gcgccgccac
     1021 agggcccggt ggtgccgcag ccgctcgcct atgccggcaa gggcgcgcaa gaaaagatca
     1081 gcgagctgca ggcgagcctg cgccgggaca atgccgacgc ggtgatcctg acccagcccg
     1141 attccatcgc ctggctcttt aatattcgcg gcgccgacgt ggcgcacaat cctgtgccgc
     1201 tggcctttgc cgtcgtgccc gccagcggca agcccgaact cttcatcgac aaaggcaagc
     1261 ttggccgcga ggcgcgcgcg catctcgccc ctttggcaaa gctcagcgcg ccggcgctgc
     1321 tcgccgcgag gatcgcggcg ctgcgggccg cgggcaagcg cgtgcggctc gacccgcatt
     1381 cggcagcgaa ctggttcttc cgcaagctac gcggcggcaa agggcgcatc gtgcgcggcg
     1441 tcgatccctg cgtgctgccc aaggcgcgca agaacgccgc cgagataaag ggcgcgcggg
     1501 cggcgcagca acgcgacggt gcggtgatgg cgcgcttttt ggcctggctc gagcgggagg
     1561 gcagcagcgg cacgctcgac gagatcggcg tcgcgcgtca attggaaaac ctgcgccggg
     1621 aaacgcaagc cctgaaggag atcagcttcg acacgatttc cggggcaggc ccgaacggcg
     1681 ccattgtcca ctaccgggtc acgaccgcga ccaaccgcac gttgcgcgcg ggcgagcttt
     1741 acctcgtcga ctccggcgct cagtacctcg acggcaccac cgacatcacg cgcacggtcg
     1801 cgctcggaag cccgacgcgc gaaatgcagg agcgcttcac actcgtgctg aagggtcaca
     1861 tcgccattgc cacggcgcgc tttccggccg gcacgcgcgg ggtggacctt gacgcctttg
     1921 cccggcgcgc gctctggcag gcgggcctcg acttcgatca cggcacggga cacggggtgg
     1981 gcagctacct gtccgtgcac gagggaccgc aaaacatttc caagcgtggc aacaccccgc
     2041 tcgagcccgg gatgatcatt tccaacgagc ccggctatta caaggccggc gcctacggca
     2101 ttcgcatcga aaacctgctg ctggtcgagg agcctcgcgc cgtgccgggc ggcgaacgcg
     2161 agatgttggg cttcgaaacc ttgacgctcg tgccgatcga tcgccgcttg atcgtgccgg
     2221 cgctgctctc cgacggcgaa cgggcctggc tcgacgccta tcacgcgcgc gtgcgcgaca
     2281 agatcggccc cgagctcggg cccgcagacc tgacctggct cacggcggcc accgcaccgc
     2341 tgccaacgcg ggccaaggcg ccctagaggc gcaggacgcg ctcgaggaac gcgagctcgg
     2401 ctgcgagcac ggccgcaatg gtttcggcgc ggcgaaagcc gtgcgcctcg ccggcgaatt
     2461 cgtgataggc cacgtcgatg ccgcgcgctt tcagccgcgc caccatgcct cgagactgct
     2521 cgggcggcac caccttgtcc tcgaggccct ggaagagaat gaccggcgcc gtgatgccgt
     2581 cgcagagctc aagcggcgaa cgcgcccgaa aaaccgcgcg ccaggtcttg ggcgttgtgc
     2641 ccatcagtcg gtggaggtag cccgattcga acttgtgggt gtgcttgagg aggagcgcga
     2701 ggtccgacac cccatagtgg cagctgccgg cggcgaaggc gcggctcgtc gccagcgcca
     2761 tgagcgtggt gtagccgccg gcgctacccc cggaaatggc gatgcgcctg gcgtcggctc
     2821 tgccggtgcg ggccaaaaac ttggccccgg ccacgcagtc ggcgacgtcg gcgatccccc
     2881 aggtcccatc gaggcgttcg cggtaggcgc ggccgaagcc cgtgctgccg gcgtaattga
     2941 gatcgaaaac ggcaaagccg cggctggtaa agaactggac gcgcagcttg aggccggcat
     3001 cggtcatgct ggtgggaccg ccgtgcacga acagcagtgc cggcggcagg gcaccggcgg
     3061 ggccccggtg cgtggcattg gccggggcat agtagatgcc atagacgcga tggtgcccgg
     3121 cgcggaaggc aagggtctcg ccggcgctga tcgagcccgt cagcgcctcg agcgcgggct
     3181 cgcgcgcgcc gggcgccgtg aagccgcgaa agccgcgtgg gccaatgcgc atgacctcgg
     3241 gcatggccgt gggacggctg atcaaggcgg caaagcctcc cgcccaggcg acggggtcgt
     3301 cgatgcgtgc ggcctggcgg gaaagtgcgg cgtaggccgt gcgcctgccc cgtttcggct
     3361 cgaccacctc caacagcggc gtgccctgct gcagcgagac ggcagcgatc cggtcctcgg
     3421 ggctcagaca atagctgcgc atgccgaaca cccattgcgg gcgcgagagc tcggcgccgc
     3481 ggcggccgag gcggcgccgt agtttgctgc cgtcccaggc gtaaagctgg ccccagccgc
     3541 tgatgtccag cacgaggtag agccagcccg tgcgcgcact ccaggttggc tgaaaggcgg
     3601 ccgcgccgtt gcctccggca atgcttttgg ccttgccgag gctgccgtct gccgcgaccg
     3661 gcgcgatcga gagccgggcg ctgtcccagg gcatatccgg cagatcccag gtcaggaacg
     3721 cgagccgctc gccgtcggga gcaaggcacg gcgaggcata aaagtcatgg gccccggcga
     3781 gctcgcgcac ggcgccgtcg gcgaggttga cggcgaggag catgttcttg ggggtcgcgc
     3841 cggcatgccg ttcgccgatc ccgatcaggc gccgacgtcg gcgatcgagc accaggtcgg
     3901 caaagcgcat gccggcggcc tcggtgaggc gcttgggcgc ctggccctgc gcaacggcgt
     3961 aaatctgctg gtcgccgtcg ttgacgaagt aaagggtgtc gtcggcgagc aggaattcgc
     4021 cgccgccata ttcgtgcacg cgcgagcggg ccgaaaaggg ggcgggcagg acttcgcgca
     4081 cgcccccgtc ggtgctgccg gcgaagatgc tctgtcgacc gccctgctcg gggcgcgatt
     4141 ggctccagta gagcgcctgc gccccagccg ccagggtgcc gaagcgtcgg ctgccggcgg
     4201 cggccaggcg ggggctcagc ggcgagggcc agcggccaaa gggggtgcgc tgcttgggca
     4261 tggggcctcg cggctggccg ggccgcggcg cgcgcgcccc ggtgggctat tttttgcaaa
     4321 aaacttggca ttggcggccg cgggcacgca aatcatcttt gcaccgcgaa ataggtcagc
     4381 tatgctgtcc tttacattgt cggcccgatc gcggcggatt gagcgccggc gggatgggcc
     4441 gccgatcacg agattctggg gtaagatcag ccgcctggac cgcggcccga tgtgctgcct
     4501 tgcggccggc gtccgatccg ggagacgcgg ccagggcagc cacacggggc gggcccaacg
     4561 gaccggtggc gtcccgctcc gcgcagcgcc gctttcgccc agcccgaagc tattgggacg
     4621 gacgccggat ctgcgtgtca agcgccagaa cctcggcagt gatggtgtct caggcagaag
     4681 gcgtcggcct atgacgtggg agagaactga cagcgccggc catccggatc ttgccgcgtt
     4741 tcaggatcgg caagcccgcg gccttttcga tccatcgcgc gagtacgatg cctgtggcgt
     4801 cggcttcatt gccaacctca aaggcggcaa gacccacaag ctgatcgaag atgcgctctg
     4861 catcctggag aatctggagc accgcggggc agtgggcgcc gatcccatcg ccggcgacgg
     4921 ggcgggcgtt ctgatccaaa tcccgcatgc cttcctggaa gcggaggccc agaagctcaa
     4981 attcaagctg ccgaagcccg ggcattatgc ctgcggtcat ttgtttctgc cgcgggacga
     5041 gcgcctgcgc gcccactgcg agcgcgtttg gacgcgcatc attcgcgagg agggcctcga
     5101 gttcatcggc tggcgctcgg tgcccgtcga caacagctgc ctttccgaaa tggtgaaggc
     5161 gaccgaaccc gtgcatcggc agatcttcat cagccggccc aaggcgatga aggagcagca
     5221 ggagttcgaa cgccgccttt atatgatccg caaggtggtc tccaacgcgc tctattatgc
     5281 ctacaagggg cgcgacatcg ggcactactc ggtgtcgctg tcgagccgca ccctggtcta
     5341 caagggcatg tttctgtcct accaggttaa gaagtactac aaggacctgt cggacccgcg
     5401 gttggcctc
//
LOCUS       DASTMT010000615        15703 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_6435984, whole genome shotgun sequence.
ACCESSION   DASTMT010000615 DASTMT010000000
VERSION     DASTMT010000615.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 15703)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15703)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15703
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6435984"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..181
                     /gene="rpsH"
                     /locus_tag="VFR00_14150"
     CDS             <1..181
                     /gene="rpsH"
                     /locus_tag="VFR00_14150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015915752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEU0160459.1"
                     /translation="IREIKRISKPGRRVYTAVRELTTVANGLGVAILSTPKGVMSDTR
                     AREENVGGEILCNVF"
     gene            218..760
                     /gene="rplF"
                     /locus_tag="VFR00_14155"
     CDS             218..760
                     /gene="rplF"
                     /locus_tag="VFR00_14155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006611853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEU0160460.1"
                     /translation="MSRIGKRPVAIPAGVTATLSGQKVTVKGPKGELSFSCPDEIAVE
                     KTTAGIAVSARTDSKPARALWGMSRTQIINLIEGVTNGYSQTLEIHGVGFRAAMRGKD
                     ALQVQVGFSHDVVHKIPTGVEVKVGGAKQEIITVSGIDKQKVGQMAAEIRAARPPEPY
                     QGKGIRYQGEYILRKEGKKK"
     gene            769..1122
                     /gene="rplR"
                     /locus_tag="VFR00_14160"
     CDS             769..1122
                     /gene="rplR"
                     /locus_tag="VFR00_14160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018044213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEU0160461.1"
                     /translation="MLRKMTQFDKRKLRVRRSLARERPRLSVFRSSKHIYAQLIDDAH
                     ATTLIAASTLEKGLKTSLKTGADKAAATAVGKLLAERAVKAGIKEVVFDRGGYRYHGR
                     IKALADGAREAGLSF"
     gene            1141..1719
                     /gene="rpsE"
                     /locus_tag="VFR00_14165"
     CDS             1141..1719
                     /gene="rpsE"
                     /locus_tag="VFR00_14165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012111675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEU0160462.1"
                     /translation="MARPYRDRDRNREERDSEFKDKLVHINRVAKVVKGGKRFGFAAL
                     VVVGDQKGRVGFGHGKAREVPEAIRKATEAARRGLVRVPLREGRTLHHDSEGRHGAGK
                     VVLRSAPPGTGIIAGGPTRAVFEMLGVQDVVAKSLGSTNPYNMVRATFDALKEQENPR
                     AVAARRGKKVSEIVARRRDGSAGEAEAAAETA"
     gene            1743..1952
                     /gene="rpmD"
                     /locus_tag="VFR00_14170"
     CDS             1743..1952
                     /gene="rpmD"
                     /locus_tag="VFR00_14170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007598359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEU0160463.1"
                     /translation="MAAAEKGKGKRLTVEQVASPQRRPKIQRQTLIGLGLNKIGRRRS
                     LEDTPAVRGMIHSVSHLVRVVEEKA"
     gene            1976..2527
                     /gene="rplO"
                     /locus_tag="VFR00_14175"
     CDS             1976..2527
                     /gene="rplO"
                     /locus_tag="VFR00_14175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEU0160464.1"
                     /translation="MRLNDIKDNPGSRKLRVRIGRGIGSGMGKMGGRGGKGQTARAGV
                     RLGGFEGGQMPLHRRLPKRGFNKLNAKEYNEINLGRLQEAVDSGRLDAGKPVDLAALL
                     AAGLVRRALDGVRVLGEGDLKAKLALTVNHATASAKAAIEKAGGTLTLIERKVLAADE
                     AKRKKSAAKKAKTGKPKAAASED"
     gene            2690..4036
                     /gene="secY"
                     /locus_tag="VFR00_14180"
     CDS             2690..4036
                     /gene="secY"
                     /locus_tag="VFR00_14180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEU0160465.1"
                     /translation="MVSAAEQLASNLNFGAFAKAEDLKRRIWFTLAALVVYRLGTYIP
                     IPGINPAAFAESFRAQSSGILGMFNMFAGGAVERMAIFALNIMPYISASIIMQLMTSV
                     VPTLEQLKKEGEQGRKQINQYTRYLTVALASFQAYGIAFGLEGSRGSAGAVVIDPGWF
                     FRISTVVTLVGGTMFLMWLGEQITARGVGNGTSLIIMSGIVAGLPSALAGLLELSRQG
                     AISWPLLIALLGLMLGVVAAIVFIERAQRRLLIQYPKRQMGNRMFQGDASHLPLKLNS
                     SGVIPPIFASSLLLLPITVAQFSQGAGPDWLNAIVASLGRGQPLYLAIYVGLIVFFAF
                     FYTAIVFNPQDTADNLRKYGGFLPGIRPGAKTAEYIDYVLTRITVVGAIYLAAVCVLP
                     ELLISYAAVPFYFGGTSLLIAVSVTMDTVAQVQSHLLAHQYEGLIKKAKLRGATRR"
     gene            4036..4683
                     /locus_tag="VFR00_14185"
     CDS             4036..4683
                     /locus_tag="VFR00_14185"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011424991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEU0160466.1"
                     /translation="MNLILLGPPGAGKGTQAKTIAANHGLIQLSTGDMLRAAVEAGSP
                     VGRKAKAVMDRGELVPDEIVVEIISARLDGADCKAGVIFDGFPRTLAQAAALDRVLKR
                     KKLKLDAVIELKVNDDQLVERIAGRFTCATCGTGYHDRFRQPKTAGICDVCGGKTFLR
                     RPDDTAETVGRRLMVYYRETSPLLGYYYCKGNLRSVDGMGSVEDVAAAIEAHLPR"
     gene            4873..5250
                     /gene="rpsM"
                     /locus_tag="VFR00_14190"
     CDS             4873..5250
                     /gene="rpsM"
                     /locus_tag="VFR00_14190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011651425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEU0160467.1"
                     /translation="MARIAGVNIPTQKRVLIALQYIHGIGPKFAQEICRKVGIPDKRR
                     VNDLTDAEVLQIREAIDRDYVVEGDLRRDVAMNIKRLMDLGCYRGLRHRRSLPVRGQR
                     THTNARTRKGKARPIAGKKKTTA"
     gene            5333..5737
                     /gene="rpsK"
                     /locus_tag="VFR00_14195"
     CDS             5333..5737
                     /gene="rpsK"
                     /locus_tag="VFR00_14195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007603045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEU0160468.1"
                     /translation="MAKETQSATRAKPRRRERKNISSGIAHVSATFNNTMITITDAQG
                     NTIAWSSAGTMGFKGSRKSTPYAAQMAAEDAGKKAMEHGMKILEVEVMGPGSGRESAL
                     RALQAVGFQITSIRDVTSIPHNGCRPRKRRRV"
     gene            5856..6875
                     /locus_tag="VFR00_14200"
     CDS             5856..6875
                     /locus_tag="VFR00_14200"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020185768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEU0160469.1"
                     /translation="MLQKNWQDLIKPTKLEVASGRQRDKVATIVAEPLERGFGMTLGN
                     ALRRVLLSSLQGAAITTVHIDGVLHEFSSIPGVREDVTNIILNIKEIALRQHSEGVKR
                     MVLKKEGPGQVKASDIEASSEIEILNPEHVICTLDQGAAIRMEFTASMGKGYVPAERN
                     RPDDAPIGLIPVDSLYSPVKKVSYRVENTREGQILDYDKLTMTVETDGSVTAEDAVAL
                     AARILQDQLAVFINFEEPKKAIEDAAKHPELAFNAALLKKVDELELSVRSANCLKNDN
                     IVYIGDLIQKTEAEMLRTPNFGRKSLNEIKEVLASMGLHLGMEVPNWPPDNIEDLAKR
                     FEDQY"
     gene            6972..7394
                     /gene="rplQ"
                     /locus_tag="VFR00_14205"
     CDS             6972..7394
                     /gene="rplQ"
                     /locus_tag="VFR00_14205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEU0160470.1"
                     /translation="MRHRKLGRRFSRDSGHRQAMFSNMAAALIRHEQIITTLAKAKDL
                     KRVMDKYITLAKRGDLNSRRLAASRMRDEAMVKKLFDTLGPRYRDRAGGYTRVLKAGY
                     RHGDSAAVAVIELVDRDELVKGKEDKERAEAMKAEANP"
     gene            7702..9126
                     /locus_tag="VFR00_14210"
     CDS             7702..9126
                     /locus_tag="VFR00_14210"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HEU0160471.1"
                     /translation="MSGLANVRRGVLVGLMSLAILPWTPAAAQQRREPPASREAAQYS
                     FAPIVKKAAPAVVNVYVRARVQTFVSPFAQDPLFGRLFGERFGLPQERIQNSLGSGVI
                     VHPDGIIVTNTHVVKVGGTAEIKVALADRREFDAKVVLQDEKSDLAVLKIEGGDGRFP
                     YLEFEDSDRAEVGDLVLAIGNPFGVGQTVTSGIISALGRTEVTQSEAQVFIQTDAAIN
                     PGNSGGALVDMAGRVLGINTAIFSRSGGSHGIGFAIPSNLVKVIVDSAISGRKLERPW
                     LGAKLDAVTRELGEALALDRVAGALVVKVYAPSAASEAGLQSGDVIVGVDGYEIADPR
                     AVYYRLTTRGIGNKCSLDVVRKGQKMTLALAVRAPPKAGRDDVRNLSGAHPFDGARVA
                     NILPGTAAELGVEEEDGVVIVSVQPSSRSARLGFRTGDIIVQVGREKIDSIGDLETQL
                     KERPRVWQVVVKRGNQLLQLQLSG"
     gene            9129..10436
                     /locus_tag="VFR00_14215"
     CDS             9129..10436
                     /locus_tag="VFR00_14215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEU0160472.1"
                     /translation="MANLFEAAGLEKGAPRPLADRLRPKKLSAVIGQDHLVGADGSLS
                     RMLASGRLQSMILWGPPGSGKTTVARLLADESQLAFEQLSAIFSGVQDLRKAFERARS
                     RRETGQGTLLFIDEIHRFNRSQQDSFLPIMEDGTITLVGATTENPSFELNGALLSRSL
                     VLVFHRLDDAALEELLRRAETEAGRALPLDGEARATLKALADGDGRTVLNMAEELMGS
                     IAPGSAPLDRAALSKLLQRRAPLYDKSRDGHYNLISALHKAVRGSDPDASLYWLARML
                     DAGEDRLFIARRLVRMAIEDIGLADPQAVLQALAAKDVFDFLGSPEGELALAQTVIYL
                     AMAPKSNAAYVAFGAALAAAKEHGSLAPPKHILNAPTRLMRQEGYGADYHYDHDAPEA
                     FSGQNYFPDGLARQRFYAPEGRGFEAELRRRLEHWQKLRRERS"
     gene            10530..11069
                     /locus_tag="VFR00_14220"
     CDS             10530..11069
                     /locus_tag="VFR00_14220"
                     /inference="COORDINATES: protein motif:HMM:NF025478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T6SS effector amidase Tae4 family protein"
                     /protein_id="HEU0160473.1"
                     /translation="MVAFKDLWYRHPANNSTQYPCVAPHGLTNLEGSFVAPGFPVFGN
                     QCSIRMGVCLKLAGVAPGLLRQPTCGVHAAEEMHYIRAADLARGLAGASLPGVGRVER
                     ITGTEASHFYAKIFGRTGIIYIQDYWKRHGETTATGDHIDVWNGYRSSTKWLMEWFSW
                     LGYYSNYAQAKEVWFWEVK"
     gene            11110..11349
                     /locus_tag="VFR00_14225"
     CDS             11110..11349
                     /locus_tag="VFR00_14225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0160474.1"
                     /translation="MRYLIAMVCAVVVAALATIFLSPRLAAMVVDRLTFTSPDEVGNL
                     EDGVFMAANFLALLLGWWLGWVLGARFLKPGETHS"
     gene            11595..12596
                     /locus_tag="VFR00_14230"
     CDS             11595..12596
                     /locus_tag="VFR00_14230"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEU0160475.1"
                     /translation="MSAPLETIQVAGNEAGLRLDRWFRVHFPEVGYSYLQKLLRSGQV
                     RVDSRRAQANLRLQAGQHVRVPQVVRTVGSSRPSVVPPPGLSKADRDFIERLILFEDE
                     HVVVLNKPFGIAVQGGTGTKRHIDGLLAGMVDRFGDRPRLVHRLDRDTTGVLLVAKHR
                     QAAAKLGRIFQTRSAAKTYWALVKGVPRPGQGKVEAALVKAPGPDGDRVRKAEPGEQE
                     QAMHATTHYAVIDRVAHKVAWVSLKPVTGRQHQLRAHMALIGHPIVGDSKYEGDKVLA
                     ESGIEAKLHLHARRLVIPHPIAGEARIDVTAPLPPHMRKTWEMLGLDAERFDAAASA"
     gene            12894..13622
                     /locus_tag="VFR00_14235"
     CDS             12894..13622
                     /locus_tag="VFR00_14235"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEU0160476.1"
                     /translation="MAAPVHFDPQIGEQFLRYARLVAARGYVHNTLGNMAIRAAHPDY
                     LHGMAYTKHAEISLEEMGLANIVVTDIPTSRLLYGSRMTSIGHNLNREILRLRPDIGA
                     VIHVHDDATIAFFGSGAFREIKVLSLDMPFILGKPPYYVPAHVDVEADVGPVKDFIAE
                     TNVLVLLGHGITALGRTISEAYHRLNSLTAEIRRNVQAEQLAALKGTSPLYRSAPEIA
                     LMYRHAEAVIYPTRAEKVMQGDAA"
     gene            13847..14812
                     /locus_tag="VFR00_14240"
     CDS             13847..14812
                     /locus_tag="VFR00_14240"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HWE histidine kinase domain-containing protein"
                     /protein_id="HEU0160477.1"
                     /translation="MTITDCPREPSDSETELGVILEGIGEGFYAVDRDWRVRRFNSEA
                     ARHFGRTATDVLGRNLWELFPGARETGLGYLFLQAMAGRKAVRSETASVVVPGRWLSY
                     GLFPLGDGLGVVFRDITDRKKAEEQRDLLLAELEHRVKNTLANVQAIAGQTFRDVEPQ
                     ARQAFEARLRALGNAQLALTAENWRGVRLRALIRSTLDPVVEQRHYAASGPNLRVQPK
                     SAVALSMAIHELCTNAIKYGALSVVGGSLSIDWTIEAGRFRLSWQEQGGPPVSAPQRK
                     GFGSLMIERALAVQVDGDVTIEYAPNGLSCRIDAPLAAIAESEDR"
     gene            complement(14906..15694)
                     /locus_tag="VFR00_14245"
     CDS             complement(14906..15694)
                     /locus_tag="VFR00_14245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013417988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HEU0160478.1"
                     /translation="MNTRRGNLSMDNAATSAGSSIVSAALIVIGDEILSGRTKDKNIG
                     HIADHLTAIGIQLKEVRIVPDEEPEIVTAVNALRSRYDYVFTTGGIGPTHDDITADSV
                     AKAFGVGIDVDERALAPMRAYFERRGVELTAARMRMARIPFGAELVENSVSIAPGFML
                     GNVIVMAGIPNIMQVMLEAATKYLKTGKKMLSAAMDLHRPEGEIADVFAALQKRYPDV
                     PMGSYPFQRNGKPGTQLVLRCTDPARLSEAENELKLQLAARGWI"
BASE COUNT         2773 a         5082 c         5173 g         2675 t
ORIGIN      
        1 gattcgtgag atcaagcgga tttccaagcc cggccgtcgc gtttatacgg ccgtgcgcga
       61 attgacgacc gttgccaacg gcttgggggt cgccattctg tcgacgccaa aaggcgtgat
      121 gtcggacacc cgcgcccgcg aggagaacgt cggcggcgaa atcctgtgca acgttttcta
      181 gacgcccgtg cccgagacga gttgaggacc aagagatatg tcgcgcatag gtaagaggcc
      241 ggtggccatt cccgcaggcg tcacggcgac gctgagcggg cagaaagtca cggtcaaggg
      301 acccaagggc gagctctcct tcagctgtcc tgacgagatc gcggtggaga aaacgacggc
      361 cggaatcgcg gtcagcgcgc gcacggacag taagccggcg cgcgcgcttt ggggcatgtc
      421 gcgcacgcag atcatcaacc tgatcgaggg cgtgaccaac ggctattcgc agacgctcga
      481 gatccatggc gtcggcttcc gtgcggcgat gcggggcaag gatgccctgc aggtgcaggt
      541 cggcttcagc cacgacgtgg tgcacaagat ccccaccggc gtcgaggtga aggtcggcgg
      601 ggcgaagcag gaaatcatca ccgtctcggg aatagacaag cagaaggtcg ggcagatggc
      661 ggccgaaatc cgcgctgcac gcccacccga gccctaccag ggcaagggta ttcgctatca
      721 gggcgaatat atcctgcgca aggaaggcaa gaagaagtag gccgcgccat gctccgcaag
      781 atgacccaat tcgacaagcg caagctgcgc gtgcgccgat cgctggcgcg ggaacgaccg
      841 cgcctgtcgg tgttccgctc gtccaagcat atttacgccc agctgatcga cgatgcccat
      901 gccacgacgc tgatcgctgc ttcaaccctc gagaagggcc tgaagaccag cctcaagacg
      961 ggcgccgata aggccgcggc cacggctgtc ggcaagctgc tcgccgagcg tgctgtcaag
     1021 gccggcatca aggaagtggt gtttgatcgc ggcggctacc ggtaccacgg gcgcatcaag
     1081 gcgctggcgg atggtgcacg cgaagccggc ctgagcttct aggcaaggaa aggcaacgag
     1141 gtggctcgtc catacagaga ccgggaccgc aatcgcgagg aacgcgacag cgagttcaaa
     1201 gacaagctgg tgcacatcaa tcgtgtggcc aaggtggtga agggcggtaa gcgtttcggc
     1261 tttgccgcgc tggtggtggt cggtgaccag aagggccggg tcggattcgg ccacggcaag
     1321 gcgcgcgagg tgccggaagc cattcgcaag gcgacggagg cggcgcgacg cggcttggtc
     1381 cgcgtgccac tgcgcgaggg tcgcaccctg caccatgact cggagggccg gcatggggcc
     1441 ggcaaggtcg tgctgcgctc ggcgccgccg gggacgggca tcatcgccgg cgggccgacc
     1501 cgggccgtgt tcgagatgct tggcgtgcag gatgtggtgg ccaaatcgct cggcagcacc
     1561 aatccctaca acatggtgcg cgccaccttc gatgccctga aagagcaaga gaacccgcgc
     1621 gcggtggcgg cacggcgcgg caagaaggtg agcgagatcg tggcccgccg gcgcgatggt
     1681 tcggccggtg aggccgaggc cgcggccgaa acagcctaac aattgaccgg aggttgcgcg
     1741 ccatggcagc ggcggaaaaa ggcaagggca agcggctgac ggtcgagcag gtcgccagtc
     1801 cgcagcgccg tccgaaaatt caacgccaga ccctgatcgg actgggcctc aacaagatcg
     1861 ggcgccgccg cagccttgag gataccccgg cggtgcgcgg catgatccac agcgtttccc
     1921 acctcgttcg cgtggtcgag gaaaaagcct aagagccgta ggagccagac gttcgatgcg
     1981 tcttaatgac atcaaggaca atccggggtc ccgcaagctg cgcgtgcgca ttggtcgggg
     2041 catcggctcg ggaatgggca agatgggcgg ccgcggcggc aaaggccaga ccgcgcgtgc
     2101 cggcgtgcgc ctgggcggct tcgaaggcgg ccagatgccg ctgcaccgcc gcttgccgaa
     2161 gcgtggcttc aacaagctca acgccaagga atataacgag atcaatctcg gacgcttgca
     2221 ggaggccgtc gacagcggcc ggctcgatgc cggcaagccc gtcgatcttg cggcgctgct
     2281 cgccgcgggc ctggtgcggc gcgccctcga cggggtgcgc gtcttgggcg agggcgacct
     2341 caaagccaag ctggccttga ccgtcaatca tgccacggcc agcgccaaag ccgcgatcga
     2401 aaaggccggc ggaacgctga cgctgatcga gcgcaaggtg ctcgccgccg acgaggccaa
     2461 gcgcaagaaa tcggcggcaa aaaaggccaa gaccggcaag cccaaggcgg cggccagcga
     2521 ggactagggc gctcggcgct gagccttggg gcgttgggac ggccgagagg aagcccgcag
     2581 gtgaggcagc acgccggctt gcgttgcacc ttcccggccc ccatgtttta acgttgccag
     2641 gccgcgcggg gcgcgcgcac cgccgccggc cagggcaggg agagagcaga tggtttccgc
     2701 cgccgagcaa ctcgcttcca atctaaattt cggggcgttc gcgaaggccg aagatctcaa
     2761 gaggcgcatc tggttcaccc tggctgcgct cgtcgtttat cggctcggca cctatatccc
     2821 gatccccggc atcaatccgg cggcctttgc cgaatccttc cgcgcgcagt cgtccggcat
     2881 tctcggcatg ttcaacatgt tcgcgggcgg cgccgtggag cgcatggcga tcttcgccct
     2941 caatatcatg ccctatatct cggcctccat catcatgcag ctgatgacga gcgtggttcc
     3001 caccctcgag cagctgaaaa aggagggcga gcagggccgc aagcagatca accagtatac
     3061 gcgctacctg accgtggcgc tggcctcctt ccaggcctat ggcattgcct tcggcttgga
     3121 gggctcgcgc ggctccgccg gcgccgtcgt catcgatccg ggctggttct tccgcatctc
     3181 cacggtggtg accctcgtcg gtggcaccat gttcttgatg tggcttggcg aacagatcac
     3241 ggcgcgcggg gtcggcaacg gaacctcgct catcatcatg tctgggatcg tcgccggtct
     3301 gccgagcgcc cttgccggcc tgctcgagct ctcgcgccag ggcgccattt cctggccgct
     3361 gctgatcgcc ttgctggggc ttatgctggg ggtggtggcg gccatcgtgt tcatcgagcg
     3421 cgcccagcgc cgcctactca tccagtaccc caagcgccag atgggcaatc gcatgttcca
     3481 gggcgatgcc tcccacctgc ccttgaagct caactcatcc ggcgtcattc cgccgatctt
     3541 tgcctcctcg ctgctgctgc tgccgatcac ggtggcccag ttcagccagg gcgccggacc
     3601 cgattggctc aacgccatcg tggcgtcctt gggccgcggc caacccctct acctggcgat
     3661 ctatgtcggc ttgatcgtgt ttttcgcctt cttctatacg gcgatcgtct tcaacccgca
     3721 ggacaccgcc gacaacttgc gcaaatatgg tggcttcctg ccggggatcc gcccgggcgc
     3781 gaagacggcg gaatacatcg actacgtctt gacgcgcatc acggtggtgg gggccatcta
     3841 tctcgccgca gtgtgcgtgc tgccggagct gctgatctcc tatgcggcgg tgcccttcta
     3901 cttcggcggg acctcgctcc tgatcgcggt cagcgtcacc atggatacgg tggcccaggt
     3961 gcaaagccat ctgctggcgc atcagtacga agggctgatc aagaaggcga agcttcgggg
     4021 cgcaacgcgg cgatgatgaa cctgattttg ctgggcccgc cgggggcggg caagggcact
     4081 caggccaaga ccatcgccgc caaccatggc ctgatccagc tgtccaccgg tgacatgctc
     4141 cgcgccgcgg ttgaggcggg cagcccggtc ggccgcaaag ccaaggccgt catggaccgc
     4201 ggcgagctcg tgcccgacga gatcgtcgtc gagatcatct cggcccggct ggatggggcc
     4261 gattgcaagg ccggggtcat tttcgacggc tttccgcgga cgctggcaca agccgcggca
     4321 ctcgaccgcg tgctcaagcg caagaagctt aagctcgacg cggtcatcga gctgaaagtg
     4381 aacgacgatc agctggtcga gcgcattgcc ggccggttca cctgcgccac ctgcgggacg
     4441 ggctaccatg accgctttcg gcagcccaaa acggctggga tctgcgatgt gtgcggtggc
     4501 aagaccttcc tgcggcgccc cgacgatacc gccgagacgg tggggcggcg gctgatggtc
     4561 tactaccggg aaacctcccc cctcctcggc tactactact gcaagggcaa tctgcgcagc
     4621 gtcgacggca tgggctcggt ggaggacgtg gcagcggcca ttgaggccca tttgccacgc
     4681 tgatgaggtt gttgacgatg cggggcggct cggttagaag aaccgcaact ttcgggactg
     4741 agaacgcagc agtcggatct ggcgcaccgc cgtcggagcc gatttgtgtg cgttttgctg
     4801 agcattgttc aggtccgatc ttgcagcaac ggcctgggcc cgttgcatgg ggcgatcctt
     4861 caggagacca gcgtggctcg tatcgcaggc gttaacattc cgacgcagaa gcgcgtcctc
     4921 atcgcgctcc aatacattca cggcatcggc cccaagtttg cgcaggagat ctgccgcaag
     4981 gtcggcatcc ccgacaagcg ccgggtcaac gacttgaccg acgcggaggt cctgcaaatc
     5041 cgcgaggcga tcgatcgcga ctacgtggtg gagggcgacc tgcgccgcga cgtagccatg
     5101 aacatcaagc ggctcatgga tctggggtgt tatcgcggcc tgcggcaccg ccgcagtctc
     5161 cccgtccgcg gtcagcgcac gcacaccaat gcgcgcaccc gcaagggcaa ggcgcggccc
     5221 atcgccggca agaagaagac aacggcctag agctcggccc gcgcgatccg gccaatgccg
     5281 gccgcgcgct caagccccca accgatcctg accccctgga gccctttgat cgatggcaaa
     5341 agagacgcaa agcgcgaccc gagccaaacc gcgccggcgc gagcgcaaga atatcagctc
     5401 cggcatcgcg cacgtgagcg cgaccttcaa caataccatg atcaccatca ccgatgccca
     5461 gggcaacacg attgcctggt cctcggccgg caccatgggc ttcaaggggt cgcgcaagtc
     5521 gaccccctat gccgcgcaga tggcggccga agatgccggc aagaaggcga tggagcacgg
     5581 catgaagatc ctcgaggtcg aggtgatggg gccgggctcg gggcgcgagt ccgccttgcg
     5641 cgccctgcag gccgtgggtt tccagattac ctcgatccgt gacgtgacct cgatcccgca
     5701 caatggatgc cgcccgcgca agcgccggcg cgtgtaggtg gcgtcgcgag cgcgacgcac
     5761 cagaagtctt ggattggaaa gacgagcccg aaggcgagga tcagcccgcg gctcgtcagc
     5821 attgagaaag agtgtgaggc ctccccgtgg caaacgtgtt gcaaaagaac tggcaggatt
     5881 tgatcaagcc gaccaagctc gaagtcgctt cagggcggca gcgagacaag gtggcgacga
     5941 ttgtcgccga gccgctcgag cgcggtttcg gcatgacgct cggtaatgcg ctgcggcgtg
     6001 tgctgctctc ctctttgcag ggcgcggcca tcaccacggt gcatatcgac ggcgtgttgc
     6061 acgagttctc ttccattccc ggcgtgcggg aggatgtgac caacatcatc ctcaacatca
     6121 aggagatcgc gctgcgccag cactccgagg gcgtgaagcg gatggtgctg aagaaggaag
     6181 ggcccgggca ggtgaaagcc tccgatatcg aggccagttc cgagatcgag atcctcaatc
     6241 cggagcacgt catttgcacc ctcgaccagg gcgccgccat ccgcatggag ttcaccgcca
     6301 gcatgggcaa gggctacgtg cccgccgagc gcaaccgccc ggatgatgcg ccgatcggcc
     6361 tgatcccggt cgatagcctc tatagccccg tcaaaaaggt ctcctatcgg gtcgagaaca
     6421 cgcgcgaggg ccagatcctc gactatgaca agctgacaat gacggtggag acggacggct
     6481 cggtgacggc cgaggatgcg gtcgcgctgg cagcccgcat cctgcaggac cagctcgccg
     6541 tcttcatcaa cttcgaggag ccgaagaagg ccatcgagga tgcggccaag caccccgagc
     6601 tggccttcaa cgcggccttg ctcaagaagg tggacgaact cgaactctcg gtccgctcgg
     6661 ccaactgcct gaagaacgac aacatcgtct acatcggcga tctgatccag aagaccgagg
     6721 cggagatgct gcgcacgcca aacttcgggc gcaagtccct caacgaaatc aaggaggtgc
     6781 tggccagcat gggcctgcat ctcggcatgg aggtgccgaa ttggccaccc gacaatatcg
     6841 aagacctggc caagcggttc gaggatcagt attgagtgcc cgctgagcgg cgctggccga
     6901 taaacccgcg ccgcttggct taatcaacgc gaggccgcag aacctcgcta gcaaacaacg
     6961 aaggacgaaa catgcgacac cgcaagctgg ggcggcggtt cagccgcgat agcggccacc
     7021 gccaggccat gttctcgaat atggccgccg cgcttatccg ccacgaacag atcatcacca
     7081 ccttggccaa ggccaaggac ttgaagcggg tgatggacaa gtacatcacg ctggccaaac
     7141 ggggcgacct caattcccgg cgtctggccg cctcgcgcat gcgcgatgag gccatggtca
     7201 agaagctgtt cgataccctg gggccgcgtt atcgggatcg ggcggggggc tatacgcgcg
     7261 tgctcaaggc cggctatcgg cacggcgact cggcggccgt tgccgtcatc gaactcgtcg
     7321 accgcgatga gttggtgaaa ggcaaagagg acaaggagcg cgccgaagcc atgaaggctg
     7381 aggcgaaccc ctgagcgagg cgtccgccac gcgcggcagc tgccgcgcgc tcaacgcgag
     7441 gatcgacggg cgccgcaggc tggcgcgccg tgcccccggg gttggcgagg gcgcccgttg
     7501 ccgcccagcc gccgccgcta cggcgtgcgc atggggcgtc cggggccctg gcttgccgct
     7561 tccagtgccg ccaagtggcc gcagaatcgc gctataggcc tgccgctcga gcgctttcgg
     7621 gacggccatg ggcgatcttc aaaggccgcg ggcgctgctc tatatttccg tgggcgcctc
     7681 aggcatgaaa agggaagcct tatgagcggt ttggcgaacg tccggcgggg cgtgctggtg
     7741 ggcctcatga gcctcgccat cctgccttgg accccggcgg ccgcccagca gcggcgagaa
     7801 ccgccggcct cgcgcgaggc ggcgcaatat tcctttgccc ccatcgtcaa gaaggccgcc
     7861 ccggcggtcg tcaacgtcta cgtgcgcgcc cgcgtgcaga ccttcgtgtc gccgtttgcg
     7921 caagaccccc tgtttggccg tctcttcggc gagcgttttg ggctgcccca ggagcgcatt
     7981 caaaattcgc tgggctcagg cgtgatcgtg caccccgacg gcatcatcgt gaccaacacc
     8041 catgtggtga aggtcggcgg caccgccgag atcaaggtgg cgctcgccga tcgccgcgaa
     8101 ttcgacgcca aggtcgtgct gcaggatgaa aagtccgacc tcgccgtgct caagatcgag
     8161 ggcggcgacg ggcgcttccc ctacctcgag ttcgaggact ccgaccgcgc cgaagtcggc
     8221 gatctggtgc tggccattgg caatcccttc ggcgtcggcc agaccgtgac cagcggcatc
     8281 atatccgccc ttggacgcac cgaggtgacc cagtcggaag cccaagtttt catccaaacc
     8341 gacgcggcca tcaatcccgg caactccggg ggggccctgg tggacatggc cggccgcgtg
     8401 ctgggcatca acacggccat attctcccgc tcgggcggat cgcacggcat cggctttgcc
     8461 ataccctcca atctcgtgaa ggtgatcgtc gacagcgcga tttccgggcg caagctcgag
     8521 cgtccgtggc tcggcgccaa gctcgatgcg gtcacgcgcg agctcggcga ggccctggcg
     8581 ctcgaccgcg tggccggggc cctggtagta aaggtctacg cgccgagcgc ggcgtcggag
     8641 gccggcttgc agtccggcga cgtgatcgtc ggcgtcgatg gttacgagat tgccgacccg
     8701 cgcgccgttt attaccgcct caccacccgc gggatcggca acaagtgcag cctggacgtg
     8761 gtccgcaagg gccagaaaat gacgctcgcc ctggcggtgc gtgccccgcc caaagccggg
     8821 cgggacgacg tgcgcaacct ctccggcgcg catcccttcg acggtgcacg cgttgccaat
     8881 atcttgcccg gcacggccgc cgagcttggc gttgaggagg aggacggcgt cgtgatcgtg
     8941 tcggtgcagc cctcctcgcg ctcggcgcgg ctcggctttc gcaccggcga cattattgtc
     9001 caggtcgggc gcgagaaaat cgatagcatc ggcgacctgg aaacccagtt gaaagaacgc
     9061 ccgcgcgtgt ggcaggtcgt ggtgaaacgc ggcaatcagc tcctgcaact ccagctttcc
     9121 ggctgaaggt ggccaatctt ttcgaagcag ccggcctgga gaaaggtgca ccgcggcccc
     9181 tcgccgaccg cttgcgtcca aaaaaactga gcgccgtcat cggccaggat catctcgtcg
     9241 gggccgacgg cagcttgagc cggatgttgg ccagcgggcg tctgcagagc atgatcttgt
     9301 gggggccgcc cggctcgggc aagaccacgg tggcgcgtct cctcgcggac gagagccagc
     9361 tcgccttcga gcagctctcc gccattttct ccggtgtgca ggatctgcgc aaagccttcg
     9421 agcgtgcccg cagtcgacgc gagacgggtc agggcacctt gctcttcatc gatgagatcc
     9481 accgcttcaa ccgctcgcag caggacagct ttctgcccat catggaggat ggcacgatca
     9541 cgctggtggg ggccactacg gagaacccgt ccttcgagct caatggtgcg ttgctgtcac
     9601 gctccctggt gctcgtcttc caccgccttg acgatgccgc gctggaagag ctcctgcggc
     9661 gcgccgagac cgaagccggc cgagccctgc cgctcgatgg cgaggcgcgc gcaacgctga
     9721 aagcgctcgc cgacggcgat ggccgcacgg tgctgaacat ggccgaggag ctgatgggct
     9781 ccattgcccc cgggagcgcg ccgctcgacc gggcggcctt gagcaagctc ctgcagcgcc
     9841 gggcgccgct ctacgacaaa tcgcgcgatg gccactacaa cctgatcagc gccttgcaca
     9901 aggccgtgcg tgggtccgac cccgacgcct cgctctattg gctcgcgcgc atgctggatg
     9961 ccggagagga ccgactgttc atcgcccgcc gcttggtgcg catggccatc gaggatattg
    10021 gtttggccga tccgcaggcc gtgctgcagg cgctcgccgc caaggacgtg tttgattttt
    10081 tgggctcgcc ggaaggcgaa ttggcgttgg cacaaaccgt catctacctg gcaatggcgc
    10141 ccaaatccaa cgcggcctat gtcgccttcg gcgccgccct cgcggcggcc aaggagcacg
    10201 gcagcctggc gccgcccaag cacatcctga acgcgccgac acgcctgatg cgccaagaag
    10261 ggtacggggc cgactatcac tacgaccacg atgcgcccga ggccttttcc ggacagaact
    10321 actttcccga tggcctggcg cgccagcgct tctatgctcc ggagggccgc ggtttcgagg
    10381 ccgagctcag gcggcggttg gagcactggc aaaagctgcg gcgggagcgg tcctagccac
    10441 tggctttgcc agggtttggc aggcgcgcgg ccattcgcct atagtcctca cgcggcccgg
    10501 atcggccggt tgcgcgtgcg gagatcacca tggttgcatt caaggacctg tggtaccggc
    10561 acccggccaa caattccacg caatacccct gcgttgcccc ccacggcctc accaaccttg
    10621 agggttcctt cgtggcgccg ggcttccccg tgttcggcaa ccagtgctcg atccgcatgg
    10681 gggtgtgcct gaagctggcc ggcgttgccc cggggctgct gcgccaaccc acctgtggcg
    10741 tgcacgccgc cgaggaaatg cactatatcc gcgccgcgga tctggcgcgg gggcttgccg
    10801 gcgcgtcgct cccaggcgtc ggcagggtcg agcgcatcac gggcacggag gcctcccact
    10861 tctacgccaa gattttcggg cgcacgggga tcatctatat ccaagactac tggaagcgcc
    10921 atggcgagac gacagccacc ggcgaccata tcgatgtttg gaacggctat cgctcgtcca
    10981 ccaagtggtt gatggaatgg ttctcgtggc tcggctacta ctccaattac gcgcaagcca
    11041 aggaagtttg gttttgggag gtgaagtagg cgcggggttt taggtcttgc agccccgtcg
    11101 ggagggagca tgcgctatct gatcgccatg gtctgcgcgg ttgtcgttgc ggcgctcgcc
    11161 acgatatttc taagcccccg cctggccgcc atggtggtgg accgactaac cttcaccagc
    11221 cctgacgagg tgggcaactt ggaggacggc gtcttcatgg ccgcgaactt cctggcactg
    11281 ctgctgggct ggtggctggg ctgggtgctc ggcgcccgct tcctcaagcc gggcgaaacc
    11341 cattcctgag ccaagcccat gcgccgtctc atgacctttc caaccgccgg cgctttcctg
    11401 ctggaagcct atggctcggc cattgcttgg cggcgctgcg gcggtgccgg ctggagcccg
    11461 gggcgcgggc ccggcgtcgc taggcgcgat ctcgcctggg aattcgggtc gcaagcgcgg
    11521 ctgcccgcag gcggcggcat ggcttgctgg ggtggggcga gcgccagccc tcgaccggcg
    11581 cggcggctct caacgtgagc gcccccctcg aaaccatcca ggtcgcaggc aatgaagccg
    11641 gcttgcgcct cgaccgctgg ttccgggtgc attttcccga ggtcggctac agctatctgc
    11701 aaaagctgct gcgctccggc caggtgcggg tcgacagccg gcgcgcgcag gcaaatttgc
    11761 gcctgcaagc cggccagcac gtgcgcgtgc cccaggtcgt gcgtacggtg ggcagcagcc
    11821 gcccctccgt cgtgccgccg cccgggcttt cgaaggcgga ccgtgacttc atcgagcggc
    11881 tgatcctgtt cgaggatgag cacgttgtcg tactcaataa gccgttcggc atcgccgtgc
    11941 agggcggcac gggcaccaag cgccacatcg acggcttgct ggccggcatg gtcgaccgct
    12001 tcggcgatcg tccccgcctc gtgcaccggc tcgaccgcga tacgaccggc gttttactgg
    12061 ttgccaagca tcgccaggcc gccgccaagc ttggccgcat cttccagacg cgctcggcgg
    12121 caaagaccta ttgggccctc gtcaaaggcg tgccgcggcc cgggcaaggc aaggtggagg
    12181 cggcgctggt caaagcgccg ggtcctgacg gagaccgcgt gcgcaaggcg gagcccggcg
    12241 aacaagagca ggcgatgcat gccaccactc actatgccgt aatcgaccgc gtggcgcaca
    12301 aggtggcctg ggtgtcgctc aagcctgtca ccggccggca gcaccagttg cgcgcgcaca
    12361 tggccctgat cggccatccc atcgttggcg acagcaagta cgaaggcgac aaggtcctgg
    12421 ccgaaagcgg tatcgaagcc aaactgcacc tgcacgcgcg ccggctcgtc atcccgcatc
    12481 ccattgccgg cgaggcaagg atcgacgtca cggccccttt gccgccgcac atgcgcaaga
    12541 cctgggagat gctgggcttg gacgccgagc gcttcgacgc cgctgcctcg gcctaagcgc
    12601 gtcgcgccgg cggccgaggc gcggagcttt cgcggccgcg cacccgctcc cccgcgtcgc
    12661 tcaaagcccc ggccatcgac ggctgggcca tgctcgccgc gcaagctgag gcgcaagcgc
    12721 ctcggcgagg ccggctggtg cggcggtcac gggaacaaca ggggatttca cgcggcacgc
    12781 gcgcccgggg ccttgcattg gccgattcgt gctgtcagcc tgacgttgcc tcggcttttc
    12841 caagcctatt ccgcgccggc cttctgccgc cgcttcgagc caaggagcat cccatggcag
    12901 cccccgtgca cttcgaccct caaatcggcg aacaatttct gcgctacgcc cgcctcgtgg
    12961 cggcccgcgg ctacgtgcac aacacgctcg gcaacatggc gatccgggcg gcgcatcccg
    13021 attacctgca cgggatggcc tacaccaagc acgccgaaat ctcgctggaa gagatgggcc
    13081 tggccaatat cgtggtcacc gatattccga ccagccggct gctctacggt tcgcgcatga
    13141 cgagtatcgg ccacaatctc aaccgcgaga tcctgcgcct gcgacccgac attggcgccg
    13201 tgatccatgt gcacgacgat gccaccatcg ccttcttcgg ttccggcgcc tttcgcgaga
    13261 tcaaggtcct atcgctcgac atgcccttca tcctgggcaa gccgccctac tacgtgcccg
    13321 cgcacgtgga tgtggaggcc gatgttgggc cggttaaaga ctttatcgcg gagaccaacg
    13381 ttttggtcct gctcgggcat ggcatcaccg ccttgggccg gaccatttcg gaggcctatc
    13441 atcgcttgaa ttcgctcacc gcggaaatcc gtcgcaacgt gcaggccgag cagttggctg
    13501 ccctcaaggg gacgagcccg ctctaccgca gcgcccccga gattgccttg atgtatcggc
    13561 acgccgaagc cgtcatctat cccactcgcg ccgagaaggt catgcagggg gatgctgcct
    13621 agcggccgtg ctcacgtctt tttctcggta tggttcggca gtggcggaca ggcagcgggt
    13681 tcagttggct ttgccgccgc tgccggcttt gctgctatag tcagccgcga cgcctctttc
    13741 gtgcccttgc cgtcggccca tcaggtgcgg ccttgtcggg gcgcgagcgc gtcgtcagtg
    13801 ggccgatgac cacagcgatc aatgtgtgcc ttgcaacgag ttcggcgtga ccattaccga
    13861 ttgtccccgt gagccaagcg actcagagac cgagcttggc gtcatcttgg aaggcatcgg
    13921 tgagggcttt tatgccgtcg atcgcgattg gcgtgtgcgg cgtttcaatt ccgaggcggc
    13981 gcgccatttc gggcgcacag cgaccgatgt gctcgggcgc aatctctggg agctattccc
    14041 aggcgcacgt gaaacgggcc ttggttacct ctttttgcaa gcgatggcag ggcgcaaagc
    14101 cgtgcgctcg gaaacggctt ccgtggtcgt gcccggacgt tggctctcct acgggctgtt
    14161 ccccttgggc gacggcctcg gcgtcgtgtt tcgcgacatt accgaccgaa agaaggccga
    14221 ggagcagcgc gaccttttgc tcgccgaact tgagcatcgc gtcaagaaca cgcttgccaa
    14281 tgtgcaggca atcgccgggc agacctttcg ggatgtcgag ccccaggcgc ggcaggcttt
    14341 cgaggcgcgc ctgcgcgcct tgggcaacgc gcagttggcc ttgaccgccg agaattggcg
    14401 cggcgtgaga ttgcgcgcgc tgatccgctc gaccttggat ccggtggtcg agcaaaggca
    14461 ctatgccgcg tcaggcccga atttgcgcgt tcaacccaag agcgcggtag ccttgtcgat
    14521 ggccatccat gaattgtgca ccaacgccat caaatacggg gcgttgtcgg ttgtcggtgg
    14581 gagcctcagc atcgactgga cgatcgaggc cggtcgcttt cgattgtcct ggcaggagca
    14641 gggggggccg cccgtatcgg cgccccagcg taaaggcttt ggctcactca tgatcgaacg
    14701 cgcgctggcc gtgcaggtgg atggcgacgt gacgatcgaa tacgcgccca atgggctatc
    14761 ctgccgcatc gacgcaccct tggctgccat cgcggaaagc gaggatcgct gaggctgctc
    14821 ggggcctgtt agccgcggct cccgcttgag cttgtgcctt cacgcagcca ggcgccgcac
    14881 ggccggcggc cacttgctgc cgggctcaga tccagccgcg cgccgccagt tgaagtttca
    14941 gctcgttttc ggcttccgac aggcgagcgg gatcggtgca acgcaaaacc agctgcgtgc
    15001 cgggcttgcc gttgcgttga aacgggtagg agcccatggg cacgtcggga tagcgttttt
    15061 gtagggccgc gaagacgtcg gcgatctcgc cctcggggcg atgcaagtcc atggcggccg
    15121 acagcatctt cttgccggtc ttgaggtact tggtggcagc ctcgagcatc acctgcatga
    15181 tgttgggaat gccggccatg acgatgacgt tgcccagcat gaagccgggc gcgatcgaca
    15241 cgctattctc caccaactcg gcaccaaagg ggatccgagc catgcgcata cgcgccgctg
    15301 tcagctccac accgcggcgt tcgaagtagg cacgcatcgg cgcaagcgcc cgctcgtcga
    15361 cgtcgatgcc gacgccgaaa gctttggcca ccgaatcggc ggtgatgtcg tcgtgcgtcg
    15421 gcccgatgcc gccggtcgtg aacacgtagt cgtaacggct gcgcagcgca ttgacggcgg
    15481 tgacgatctc cggctcctcg tccggcacaa tgcgcacttc cttcaactga atgccgatgg
    15541 cagtcaggtg atcggcgatg tgcccgatat tcttgtcctt ggttcggccg gacaggattt
    15601 cgtcgccgat cacgatcagg gcggcactca caatcgagga accggcgctt gtcgcagcat
    15661 tatccatgga gaggttgcct cgccgagtgt tcatgttgca ggc
//
LOCUS       DASTMT010000616         2715 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_4508878, whole genome shotgun sequence.
ACCESSION   DASTMT010000616 DASTMT010000000
VERSION     DASTMT010000616.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2715
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4508878"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(74..703)
                     /locus_tag="VFR00_14250"
     CDS             complement(74..703)
                     /locus_tag="VFR00_14250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEU0160479.1"
                     /translation="MSGSPTPFARRSLGVLSRIAPDRRRHKRLAVTLLGRFMRANKQE
                     YPCRLMDASAGGAALQSPVGVELGERVVAYFDHLGGIEGPVVRGCEGGFAFRIEATHH
                     KREKLAAQLTWLANRADMADTAERRHERIAPSNTVSQLALAEGVMLACKVLDVSISGA
                     SIETPARPEIGTEVMLGKLRARVMRHHAQGFGVQFIDIQNPAALRRYFG"
     gene            complement(902..1537)
                     /locus_tag="VFR00_14255"
     CDS             complement(902..1537)
                     /locus_tag="VFR00_14255"
                     /inference="COORDINATES: protein motif:HMM:NF018960.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEU0160480.1"
                     /translation="MKQKSPNTLYGYWNELRAGRMAPRRLEVEPSRIASILSETFMLE
                     RVNAADYQYRLAGTRLCELFGTELRSSNFLAGWQEADQAVLAHQLALICEQGAVVALE
                     LETTGAARHALEFEAILLPLLHTGNSINRIIGAMCLTSPAPILGSERAWRRKLRKYEV
                     IWPDGRPRAVLERCGIDGPFQAPQHSPAPKGERPQLRVLDGGRTLWKKEPS"
     gene            1765..2505
                     /locus_tag="VFR00_14260"
     CDS             1765..2505
                     /locus_tag="VFR00_14260"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEU0160481.1"
                     /translation="MPLDRHEPIFNVPGVVLLVIGLCTLLQVVRWLLPAGEDAWLTAA
                     LAFIPMRLVPGVAEQLPGGQLATLTSFVTHLFVHGDTTHLLINMAWLLAFGSPLARRT
                     DAWRFLAFFAICGAAGALFYVLVNGRVFTLVVGASGAISGLMGGAMRFFFRALKPGGR
                     IILPLHAAPLLSLSATFRDRRIVLAMIGWTILNIVLAWGAAGLTDAAGIAWEAHVGGF
                     YAGLLMFGLFDRPAPLPEVEGGNAAGAE"
BASE COUNT          429 a          870 c          906 g          510 t
ORIGIN      
        1 ccgcgcccag acaccctaac ttaacgatct gtagcggttc cggttccggc tgcatggcag
       61 gccatccact ctctcaacca aaatagcggc gtagtgcggc cgggttctgg atatcgatga
      121 attgcacgcc gaagccttgc gcgtgatggc gcatgacgcg ggcccggagt tttcccagca
      181 tcacctcggt accgatctcc ggccgggccg gggtttctat ggaggcgccc gagatcgaca
      241 cgtccaaaac tttgcacgcc agcatgacgc cctccgccag cgccagctgg ctgaccgtgt
      301 tgctaggcgc gatgcgctcg tgccgccgtt cggccgtatc cgccatatcg gcgcggttgg
      361 cgagccaagt gagctgggcg gcgagcttct cacgcttgtg atgcgtggcc tcaatgcgaa
      421 aggcgaagcc gccctcgcag ccgcgcacca ctgggccctc gatgccaccg aggtgatcga
      481 aataggcaac gacgcgttca ccgagttcga cgccgaccgg ggactgcagc gccgcgccgc
      541 cggcggatgc atccatcagc cggcatgggt actcctgctt gttggcacgc atgaagcggc
      601 cgagcagggt gaccgcgagc cgcttgtggc gtcgacgatc cggcgcaatg cgcgacagga
      661 cgcccagcga gcggcgggcg aagggcgtag ggctgcctga catcgaaacc cgttgccttg
      721 ttcaggtcga tctagctgag gggtcctcgg tggtccgtgc aaggtgcgca cgagccgcgg
      781 cgatggaccg ccgagagcac tatcaagggt ttgatttaat gaacgcctaa cgcctgggcc
      841 gattggggac aggctgctgg cgttgtcgcg gcaggcgcat ctgtcggtgc gggcaaggtg
      901 ctcaagacgg ctccttcttc cagagcgtgc gacccccgtc gaggacgcgc aactgtggtc
      961 gctctccctt gggcgccggc gaatgctgcg gcgcctgaaa cgggccgtcg atgccacaac
     1021 gctcaagcac ggcgcgcggc cggccgtcgg gccagatcac ctcgtacttg cgcaacttgc
     1081 ggcgccaggc gcgctcgctc ccaaggatgg gcgccggcga ggtcaggcac atggcgccga
     1141 tgatgcgatt gatgctgttg ccggtatgca gaagcggcag cagaatggct tcgaattcca
     1201 gcgcgtggcg ggccgctccc gtggtctcga gctcgagcgc gacgaccgcg ccctgctcgc
     1261 aaatcaaggc cagttggtgc gccagcaccg cctgatcggc ctcctgccag ccagcgagaa
     1321 agttgctcga gcgcagctcg gtgccgaaca gctcgcaaag gcgcgtgcca gccagccggt
     1381 attgatagtc ggcggcgtta acgcgctcga gcatgaaggt ctcggacaga atggaggcga
     1441 tgcgcgaggg ttcgacttcc aagcggcgcg gcgccatccg gccggcgcgc agctcgttcc
     1501 agtagccgta gagcgtattc ggggattttt gcttcatgcc gaacgaccga ccgcagcttg
     1561 catggtgaac gtcttggcgt gccggcacct gtagcgaaaa aagacggggg cctgtcgccg
     1621 cagcccgaaa agagcagtat tcgtgccagt caggcctcac tgaagcacgc gccgacccgt
     1681 cgcggggcgg cacttgcagt taaggttgtc agccttcctg gcagtgtgta agcgggctca
     1741 cgccaacccc cgccttggag tgcgatgccg ttggaccgcc atgaaccgat ttttaacgta
     1801 cccggcgtcg tgctcctggt gatcggcctg tgcacccttc tgcaggtggt gcgctggctg
     1861 ctgccggcgg gagaggacgc ctggctgacc gccgcgctcg ccttcattcc gatgcggctc
     1921 gtgcctggcg tcgccgagca actgcccggc gggcagctcg ccacgctcac ctccttcgtc
     1981 acgcatctgt tcgtgcacgg cgacaccacg catctcctca tcaatatggc gtggctgctg
     2041 gccttcggca gtccgctcgc ccgccgcacc gatgcctggc gctttctcgc ctttttcgcc
     2101 atttgtgggg ccgccggcgc cctgttctat gtgttggtta atggccgggt tttcacattg
     2161 gtggtgggcg cttccggcgc catttccggg ttgatgggcg gcgccatgcg ctttttcttt
     2221 cgcgcgctta aacccggcgg caggatcatt ctgccgctgc atgcggctcc gctcttgagc
     2281 ctgtcggcca ccttccgcga ccggcgcatc gtcttggcga tgatcggctg gaccatcctc
     2341 aacatcgtct tggcctgggg ggcggccggg ctaaccgatg cggcagggat cgcctgggaa
     2401 gcccacgtcg gcggttttta cgcgggattg ctgatgtttg gcctgttcga ccgcccggca
     2461 cccttgccgg aggttgaggg gggcaatgct gcgggcgcgg aataggtctc ttgcggcgcc
     2521 tgctgaagtt gctcacaatc cccggtcata ctcgcgctaa ccgcacagca ggcgcacagg
     2581 agtgcggcgc aggaggacgg taagctcgtt cggggggcct cttgttggca cctgtccgct
     2641 ggcggtgaca gcgaatggga ggacattgcc atgaacgttg ccgccatcct tcggcaaaag
     2701 ggtcgcgccg tcacc
//
LOCUS       DASTMT010000617         9000 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_5166569, whole genome shotgun sequence.
ACCESSION   DASTMT010000617 DASTMT010000000
VERSION     DASTMT010000617.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 9000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9000
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5166569"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..194)
                     /locus_tag="VFR00_14265"
     CDS             complement(<1..194)
                     /locus_tag="VFR00_14265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0160482.1"
                     /translation="MELGPVKTDDASVKALNYILEAWEEGTESGIAPELMAYAALYTA
                     LTDLVASFGEESVVSLVASLG"
     gene            complement(380..772)
                     /locus_tag="VFR00_14270"
     CDS             complement(380..772)
                     /locus_tag="VFR00_14270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02301 family protein"
                     /protein_id="HEU0160483.1"
                     /translation="MLLLPRAAVTLSLAACLSTAALAQPQVGSLENRPYDDKLLRLSE
                     ILGAVHYLRELCGNNDGQLWRDRMKDLINAEGSSALRRAKLTRSFNDGYRSYSRTYYS
                     CTASAQTAIARFLTEGAEIADTLVKTVP"
     gene            complement(869..1336)
                     /locus_tag="VFR00_14275"
     CDS             complement(869..1336)
                     /locus_tag="VFR00_14275"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEU0160484.1"
                     /translation="MRSGAKTHRPAPANGAPWPRCAASAAIFRAETVLLIERGSGALK
                     GLWSLPGGHIEPGERAAAAASRELYEEAHVEAELAGLLDIHEVVLRDAGSQLLAHYLI
                     TVFFGRWVTGEPVAGGDAAAARFIALDELAQYPLTDGAAALILRAWKRLGEAA"
     gene            1502..2284
                     /locus_tag="VFR00_14280"
     CDS             1502..2284
                     /locus_tag="VFR00_14280"
                     /inference="COORDINATES: protein motif:HMM:NF012760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyl transferase"
                     /protein_id="HEU0160485.1"
                     /translation="MLSGDGKSRPRIAVLIAGGRLAHMLVNRLETQFGALLVLKEEPE
                     SKLAIVRRRARLIGWRQALGQMAFGLLQRLVQRRSAQRLAALWRAHGLNPEPPRSGWQ
                     EIGSVNGSACRQALRQFRPDVVVVYGTRIIRWRTLGALAAPFINYHAGINPKYRGQNG
                     AYWARSRADDAHAGVTIHLVDEGVDTGAVLYQAQVQFARDDNIATYQHRQMAVALPLL
                     TRAVEDALAGQLRPRRVNLPSQQWFHPTLWGYLKTGILMHVW"
     gene            complement(2301..3353)
                     /locus_tag="VFR00_14285"
     CDS             complement(2301..3353)
                     /locus_tag="VFR00_14285"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU0160486.1"
                     /translation="MTNRIGMATQLAGQSLRFGWYFTLNRLLDWRSAQLGKGARPRPG
                     GPAPSLSELLASQAELLLSDALAVRDGLYPAMEDDATPPLRHLARIGRMFADLPEALS
                     RRAANDATSVQAEPGAAELPAYYAQDFHFQTGGYLSEGSATLYDVQVETLFMGAAGPM
                     RRAALAPLAEALSGRDQRRLTLLDVACGTGRFLRQVRLAYPALRLKGLDLSRAYLQEA
                     RHHLGELRPIEWIEAAAERMPLAAASVDVVTCIFLFHELPPEVRRAATAEIARVLRPG
                     GVFIFLDSLQLGDKPAWDGLLQSFPYRFHEPYYQGYLSDGLEGVFEAHGLEAVLTSTP
                     FLSKLMVRRKALASAH"
     gene            3474..4106
                     /locus_tag="VFR00_14290"
     CDS             3474..4106
                     /locus_tag="VFR00_14290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015669721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEU0160487.1"
                     /translation="MQQPPASDVAFSPAVKAAQARRGSREGFAQLEQKGGFRTLITPE
                     LAEFLASARSFYFATASAEGQPYMQHRGGPQGFLRVLDASTLGFADYAGNRQYITTGN
                     LSENPRAMLFVMDYRARRRVKLWGTARILAEPAQAAPQLWAELCPQGYAARLEQAILF
                     DVVAWDMNCAQHIPRLLAAADVGETIRQFQARIRELEAEVERLKGAAPPP"
     gene            complement(4218..4961)
                     /locus_tag="VFR00_14295"
     CDS             complement(4218..4961)
                     /locus_tag="VFR00_14295"
                     /EC_number="2.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004057 - arginyl-tRNA--protein
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016598 - protein arginylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginyltransferase"
                     /protein_id="HEU0160488.1"
                     /translation="MTEQQKHFPEFYVTAPQPCPYLAGRLERKLFTHLTHDKPPALVD
                     NLLKGGFRRSQNIAYMPYCEGCQACISVRVLVDEFTPSRSMRRVIDMNRDLAARRLQA
                     TPTSEQYALFRSYIDARHAEGGMADMTVLDYAMMVEDSIIDTFLTEYRLPFAGPSNGR
                     REKAPLVAAALCDRLSDGISMVYSFYDPEAGRRGLGTYMILDHIAYAKELGAPYLYLG
                     YWINGSPKMSYKRRFQPQEQLTGNGWIRG"
     gene            complement(5226..6281)
                     /gene="hemB"
                     /locus_tag="VFR00_14300"
     CDS             complement(5226..6281)
                     /gene="hemB"
                     /locus_tag="VFR00_14300"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009491095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HEU0160489.1"
                     /translation="MTEMKPLARRSPQAARALKEPPGEAGSFPAVRLRRNRMHGWSRR
                     LVAENVLTSADLIWPLFVSEGNGTRAPVASMPGVERISVDLAVAAAREAAVLGIPAIA
                     LFPYTDPKLRSEDGREAFNEDNLVCRATRAIRKAKIDVGILLDVALDPYTSHGHDGLM
                     LEGEIVNDLTLQTLVRQSLVQVEAGCDIIAPSDMMDGRVGAIRKALEAHGHANVQIMA
                     YAAKYASAFYGPFRDAVGSSATLKGDKRTYQMDPANSDEAIREVALDIAEGADMVMVK
                     PGMPYLDIVRRVKETFGVPTFAYQVSGEYAMLTAAAQNGWLEGDKVMLESLLAFKRAG
                     ADGVLTYFAPRAAKLLA"
     gene            complement(6377..7465)
                     /locus_tag="VFR00_14305"
     CDS             complement(6377..7465)
                     /locus_tag="VFR00_14305"
                     /inference="COORDINATES: protein motif:HMM:NF018597.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extensin family protein"
                     /protein_id="HEU0160490.1"
                     /translation="MGAVSMRGMSSVAAAGWLLLAAQAASAQGVPPAAEPGTAPRPGA
                     SHKPAGAPTPASPAPASPAPAASEPGWSAQEIELAQARCAVLLKGLPVVAVPEGPLRE
                     GNECGAPAPMRLISVGKDPQVAFSPPPTLTCDMIAQLSKWVERDVQPLAKKFLAAPIV
                     RIETMSSYSCRNAYGRAHGRLSEHGRANAVDIGSFVTAQGQAALVISDWGPTAREIAA
                     AAAAEVRAAAQASAPAPRQAKGEPAPPAQPVASVAPTAEPAGRAAAAMTGFSLSIPGV
                     MVQIGGGSDKSADSAFAPAQRLGGPKAKTAGAAAIPNPDARLDFLRAVHAAACRQFGT
                     VLGPEANNWHKNHFHVDMAERPRGVICE"
     gene            7644..8738
                     /locus_tag="VFR00_14310"
     CDS             7644..8738
                     /locus_tag="VFR00_14310"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF004127.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEU0160491.1"
                     /translation="MHSLLQEANELLIAQQGAAGRITLNRPVRRNALSTAMRAAIAAA
                     LPGWVRDPNIYGVVVASAAPGVFCAGGDLRELIDWGRNAKAEARSSLAAEYALNWRLE
                     CFTKPTVSLIDGLVVGSGVGLSLYGTHRVAGENYRFAMPETHIGLFPDDGVCHAFARM
                     PDHLGTFLALTGRAIGPADAYPLGLATHVIPAADFGAIAAAMAEAEPVDQVLDSRHQP
                     PRPGELTEGVREVVARCFAADTVEEIIARLQSHGGPRQDWAQAVAGELTRRSPTSLKL
                     THRHLIQARGLDLRATLVQDYRIAHQCLEGEDLYEGVRAVVIDKDGAPRWRPGRLADV
                     TEALLAPYFAAPVGGDLALASRAEMQALQS"
     gene            8856..>9000
                     /locus_tag="VFR00_14315"
     CDS             8856..>9000
                     /locus_tag="VFR00_14315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEU0160492.1"
                     /translation="MNLVLEAARPRAPVGEDTFRSEYLRALRLIERLHRLLLDVIKDE
                     FDRR"
BASE COUNT         1522 a         3004 c         3007 g         1467 t
ORIGIN      
        1 ccgaggctgg cgaccaggct caccaccgat tcctcgccga acgaggcaac gaggtcggtc
       61 aacgcggtat aaagggcggc gtaggccatg agttcggggg cgatcccgct ttcggtgcct
      121 tcctcccatg cctccaggat gtagttgagt gccttcacgc ttgcgtcgtc ggtcttcacc
      181 ggcccaagtt ccattccagt ctctccccat ttcccccatg atcggctgtg cagcgcgcgt
      241 gcccgcctca cgcccacccc ccaacagcca atcgtcgacg ccgcttgcgc ggcacccccc
      301 gtgcctatca cggccccggg ggtcgggtat ctgctacaac agttaacgat taattaacgt
      361 cgtccttgcc cgctgacgct taaggcacag tcttgaccag ggtgtccgcg atttcggcgc
      421 cttcggtcag aaagcgggca atggccgtct gcgccgaggc cgtgcagcta taataggtgc
      481 gactgtagct gcggtagccg tcgttgaagc tgcgcgtcag cttggcgcga cgcagagcgg
      541 aagagccttc ggcgttgatc agatccttca tgcgatcgcg ccagagctgg ccgtcattgt
      601 tgccgcacaa ctcgcgcaaa tagtgaaccg cgccgaggat ttccgacagc cgcaacaact
      661 tgtcgtcgta gggacggttt tccagcgagc cgacctgggg ctgcgcgagc gcggcagtgc
      721 tgaggcaggc cgcgaggcta agcgtcacgg cggcgcgggg caggagcagc atgggaccta
      781 atgtcggggc aactgcaagc caagaaaagg ggccggttcg caacggacgc atagagacag
      841 gcggtgagtt ccccggcgcg cctgcacatc aggctgcctc gccgaggcgc ttccaggcgc
      901 gcaaaatcaa ggctgccgcc ccgtcggtca agggatattg cgccagctcg tcgagtgcga
      961 taaagcgcgc cgcggcggca tcgccgcccg ccaccggctc gcccgtgacc cagcgcccga
     1021 agaagacggt gatgagatag tgggccaaca gctggctgcc ggcgtcccgc aacaccactt
     1081 catggatgtc gagcagaccg gccagctcgg cctcgacgtg ggcttcctcg taaagctcgc
     1141 gactagccgc cgccgccgcc cgctcgccgg gctcgatatg cccgccgggt aaactccaca
     1201 gccccttgag cgcaccgctg ccgcgctcga tcaacaagac ggtctcggcg cggaaaatgg
     1261 cggcactcgc cgcgcagcgc ggccacggcg cgccgttggc cggcgccggt cgatgtgtct
     1321 tcgcaccgct cctcatggcg ctcgagctta gcccgccagc cgccccggcg tcgatcgatt
     1381 gcctggacca gaacgagcgc aggactagcc cagaactggc ccagaactgg cccagaactg
     1441 gcccagaact tgcctcgtgc gccgtgggcg tcgagggttg gtcgcaagac gggacgacag
     1501 gatgctgagc ggcgatggca agtctcggcc gcggatcgcg gtgctgatcg cgggcggacg
     1561 gctggcgcac atgctggtta accggctcga aacgcagttc ggagcactgt tggtcctcaa
     1621 ggaagagccc gaatccaagc tcgcgatcgt acggcgacgc gcccgtttga tcggctggcg
     1681 gcaagccctc ggtcaaatgg ccttcgggct gctgcagcgg ctggtgcagc ggcgcagcgc
     1741 ccaacgactg gccgcgctct ggcgcgcgca cggtctcaat cccgagccgc cgcgctcggg
     1801 ttggcaggag atcggctcgg tcaatggctc cgcctgccgt caggccttgc ggcagtttcg
     1861 gcccgacgtg gtggtcgttt acggcacgcg catcatcagg tggcgcacgt tgggcgcgct
     1921 ggcggcaccc tttatcaatt accatgccgg catcaacccc aagtaccgcg gccagaacgg
     1981 ggcctattgg gcgcgcagcc gggccgacga tgcccatgcc ggggtcacca tccatctggt
     2041 cgatgaaggc gtcgacaccg gcgccgttct ctaccaggcg caagtccagt tcgcccgcga
     2101 cgacaacatc gccacttacc agcaccggca gatggccgtg gccttgccgc tgttgacccg
     2161 ggccgtggag gacgcgctgg ccggacagct gcggcctcgg cgcgtaaacc tgccctcgca
     2221 acagtggttt catccgacgc tgtggggcta tttgaaaacc ggaattctca tgcacgtgtg
     2281 gtgagcgcgc tcgcaggccc tcagtgggcc gaggcaagtg ccttgcggcg caccatgagc
     2341 ttggagagaa aaggcgtact ggtgagaacg gcttcgaggc catgcgcctc gaacacaccc
     2401 tcgagcccgt cgctcagata gccttgatag tagggctcgt ggaagcggta gggaaagctt
     2461 tgcaacaagc catcccacgc cggcttgtcg ccgagctgca ggctgtcgag aaaaatgaac
     2521 acgccgcccg gccgcaacac gcgtgcgatc tccgccgtcg ctgcccggcg cacctccggc
     2581 ggcaattcgt ggaagaggaa gatgcaggtg accacgtcga cgctggcagc tgccaagggc
     2641 atgcgctcgg ccgccgcctc gatccactcg atcggacgca gctcacccaa atggtggcgc
     2701 gcttcctgca gataggcgcg ggataaatcg aggcctttga ggcgcagggc cggataggcg
     2761 agccgcacct ggcgcaaaaa gcgaccggtg ccgcaggcca cgtcgaggag cgtgaggcgg
     2821 cgctgatcgc gtccgctcag ggcctcggcg agcggagcca aagccgcgcg ccgcatcggg
     2881 ccggcggccc ccatgaacag cgtctccact tgcacgtcgt aaagcgtggc cgagccttcg
     2941 ctgagatagc cgccggtttg gaagtggaag tcttgcgcgt agtaggccgg aagctcggcg
     3001 gcgcccggct cggcctgcac gctcgttgca tcgtttgcgg cccggcggct cagcgcctcg
     3061 ggcaggtcgg cgaacatgcg accgatgcgg gccaagtgcc gcagcggcgg ggtggcgtcg
     3121 tcctccatgg ccggatagag cccgtcacgc acggcaagcg cgtcgctcag caagagctcg
     3181 gcttgactgg caagcagctc gctcagcgac ggcgcggggc cacccggccg cgggcgcgct
     3241 cccttaccca gctgcgcgct gcgccaatcg agcaggcgat tgagcgtgaa gtaccagcca
     3301 aagcgtagcg attgacccgc aagctgcgtc gccatgccga tccggttggt catgcgtgtg
     3361 ccaccattgc gccgcacgcc tgagacccgc tcaggctcgg ggcaacaaaa actcaagata
     3421 caaggtgcca ggtcacgggt tagtttgccc ctcggcaacg agaatcgcaa accatgcagc
     3481 agcccccagc aagtgatgtc gctttcagcc ccgccgtcaa agccgcgcag gcgcggcgcg
     3541 gttcccgcga gggcttcgcc cagctggagc aaaagggtgg ttttcgcacc ctgatcacgc
     3601 ccgagctggc ggagtttctc gccagcgccc gctcgttcta cttcgccacg gcgagcgccg
     3661 aggggcagcc ctatatgcag caccgcggcg gaccgcaagg attcttgcgc gtgctcgatg
     3721 ccagcacgct tggctttgcc gattatgccg gcaatcgcca atacatcacg accggcaatc
     3781 tcagcgaaaa cccacgcgcc atgctctttg tcatggacta ccgggcccgc cggcgcgtga
     3841 aactgtgggg cacggcgcgc atccttgccg agcccgccca ggcggcgccg cagctatggg
     3901 ccgagctctg tccgcagggt tatgcggcgc gcctggagca ggcaatcctg ttcgatgtgg
     3961 tggcctggga catgaattgc gcccagcaca ttccccgtct gctagcggcc gcagacgtgg
     4021 gcgagaccat ccgccagttt caggcgcgca ttcgcgagct tgaggcagaa gtggagcgcc
     4081 tcaagggggc agcgccgccg ccgtgagggc gcacttttca tcgggctgcg cccgcaggcc
     4141 atccgtgcca aactttgccc cgcggcgccg gcagcgaggg ctcacacgtc tcgggcagaa
     4201 atcgaagcga ccggcgctca gccgcggatc caaccgttac cggtcagttg ctcctgcggt
     4261 tggaagcgcc gcttgtagct cattttcggc gaaccgttga tccaataccc cagatacagg
     4321 tagggcgcgc ccagctcttt ggcataggcg atgtgatcga ggatcatgta cgtgccgagg
     4381 ccgcgccgac cggcctcggg atcatagaag ctgtagacca tggaaatgcc gtcggagagg
     4441 cggtcgcaca gcgcggcggc gacgaggggc gccttctcgc gtcggccatt gctcggaccg
     4501 gcgaacggca ggcgatattc cgtgaggaag gtgtcgatga tgctgtcctc gaccatcatg
     4561 gcatagtcga gcaccgtcat gtcggccatg ccgccctcgg catgtcgggc atcgatgtag
     4621 gagcgaaaga gcgcgtattg ctccgaggtc ggggtggcct gcaaccgcct cgctgccaga
     4681 tcgcggttca tgtcgatcac acgccgcatg gagcggctgg gtgtgaactc gtcgaccagc
     4741 acgcgcacgg agatgcaggc ttggcagccc tcgcagtagg gcatgtaggc gatgttctgg
     4801 gaacggcgaa aaccgccctt caacaggttg tcgaccagcg ccggcggctt atcgtgcgta
     4861 agatgggtga agagcttgcg ctcgaggcgg ccggcgagat aggggcaagg ctgcggcgcc
     4921 gtgacgtaga actcggggaa atgcttctgc tgctcggtca tgcgtacgcg tcactcggcg
     4981 ctacccgaag caggaccggc gtggcgcgaa gccctgtggc ttccggtgct cggcaggttt
     5041 gcttcgaacg ttacaggtag agcattcgcc gtcaaaacgg tggtgaagca cacgtcaacg
     5101 cgcacggcga tcctgcccta ggctctcgcc gatgataggt gaggggcgcg ccttcaagca
     5161 agagcgacgc gacacgccgg tcgtcgccta tgcaacgttt cttcggttgg ccgcggttgg
     5221 ctgccttaag ccagcaactt ggcggcacgc ggcgcaaaat aggtgagcac gccatcggcg
     5281 ccggcgcgct taaaggccaa gaggctctcc agcatcacct tgtcgccctc gagccagccg
     5341 ttttgggccg ccgcggtcag catcgcgtat tcgccggaca cctgataggc aaaggtcggc
     5401 acgccgaacg tctcctttac gcggcgcacg atgtcgaggt agggcatgcc gggcttgacc
     5461 atcaccatgt cggcgccttc ggcgatgtcg agggcgacct cccgtatcgc ctcatcgctg
     5521 ttggcgggat ccatctggta ggtccgcttg tcgcctttga gcgtcgccga ggagcccacc
     5581 gcgtcgcgaa acggcccgta gaaggcggag gcatacttgg ccgcgtaggc catgatctgc
     5641 acgtttgcat ggccgtgcgc ctcaagcgcc ttgcggatgg cgccgacccg cccatccatc
     5701 atgtcggagg gcgcaatgat gtcacagccg gcctcgacct ggaccaagct ctggcgcacg
     5761 agcgtttgca aggtcaggtc gttgacgatt tcgccctcca gcatcagccc atcgtggccg
     5821 tggctggtgt aaggatcgag ggcgacgtcg agcaggatgc cgacgtcaat cttggccttg
     5881 cgaatggccc gcgttgcccg acacacgagg ttgtcctcgt tgaacgcctc gcggccatcc
     5941 tcgctgcgca acttgggatc ggtgtaggga aagagcgcga tcgccggaat gcccaggacc
     6001 gccgcttcgc gcgccgctgc cacggcaaga tcgacgctga tacgctcgac gcctggcatg
     6061 gaggcgacgg gcgcgcgggt gccgttgcct tcgctcacga acagcggcca gatcaggtcg
     6121 gcgctggtca ggacattctc cgccacgagc cggcgcgacc agccgtgcat acgattgcgg
     6181 cgcaggcgca ccgctggaaa gctgccggcc tcgccaggag gctccttgag agcgcgtgcg
     6241 gcttgagggg agcgccgcgc cagcggcttc atttcggtca tggtggctcc ttgccccgct
     6301 ccttgggcgc gtcgcctcgc cgcaggcgcg ccgcgcgcaa cgtcacgcca agtctagcgc
     6361 tgagcccgcg gctcgactac tcgcaaatca ccccgcgcgg gcgctcggcc atgtcgacgt
     6421 gaaagtggtt cttgtgccaa ttgttggcct caggaccgag cacggtgcca aactggcggc
     6481 aggccgcggc atgaacggcg cgcaaaaagt ccaggcgcgc atcggggttg gggatggcag
     6541 cggcgcccgc ggtcttggct tttggaccgc ccaggcgttg cgccggcgca aaggccgaat
     6601 cggcggattt gtcggagccg ccgccgatct gcaccatcac cccgggaatg ctgaggctaa
     6661 agccggtcat cgccgccgcc gcccgtcccg caggctcggc ggtgggcgcc acgctcgcca
     6721 ccggctgcgc gggcggggca ggctcgcctt tggcttggcg cggcgccggc gcgctcgcct
     6781 gggccgctgc tctcacctcg gcggcagcgg cggcggcaat ctcgcgcgcg gtggggcccc
     6841 agtcggagat caccagcgcc gcctgaccct gcgcggtcac gaaggagccg atatcgacgg
     6901 cattggcgcg tccatgctcg ctcaagcgtc cgtgcgcgcg accataggcg ttgcgacagg
     6961 agtaggagct catggtctcg atgcgcacga tcggggcggc caggaacttc ttcgccaagg
     7021 gctgcacgtc ccgctccacc cacttgctga gttgggcgat catgtcgcag gtcagcgtcg
     7081 gcggcggcga aaaggccact tgcggatctt tgccgacgct gatgaggcgc atgggcgcgg
     7141 gcgcgccgca ttcgttgccc tcgcgcaggg gcccttccgg cacggccacg acggggagac
     7201 ctttgagcag gacggcgcag cgcgcctggg caagctcaat ctcctgggcc gaccagcccg
     7261 gttccgacgc cgcgggcgca ggactcgcgg gcgcaggact cgccggcgta ggcgcgccgg
     7321 caggcttatg gctcgcgccc ggccggggcg ccgtaccagg ctccgctgcc ggcggcacac
     7381 cttgggctga ggccgcttgc gcggccagca ggagccagcc tgcagccgcc accgagctca
     7441 tgccacgcat tgagacagcc cccatttcga taccaggccc cgaagcaacg cccccacgaa
     7501 tcggaaccag gagtatgctg acatcaatcg gtcatacata gccccgaatt gtggccggca
     7561 cgactttcgc gctcgacctt gcacaagggc ggaacaagtt gtggaccaga gtgggtccct
     7621 tacgaccgac gcaaccgcgc aacatgcaca gtttattgca agaagctaac gaactcctaa
     7681 tcgcccagca gggcgcggcg ggacgcatca ctctcaaccg acccgtgcgc cgcaatgcct
     7741 tgagcaccgc catgcgcgct gcaatcgccg cggcgctgcc aggctgggtg cgcgacccca
     7801 acatctacgg cgtcgtcgtc gcttcggccg cgcccggggt gttttgcgcc ggcggcgacc
     7861 tgcgggagct catcgactgg ggccgcaacg ccaaggccga ggcgcgcagc tcgctggccg
     7921 ccgaatacgc gctcaattgg cggcttgagt gctttaccaa gccgaccgtc tcgctgatcg
     7981 acggcctcgt ggtcggctcc ggggttggcc tcagcctcta tggcacgcat cgcgttgccg
     8041 gcgagaacta ccgtttcgcc atgcccgaaa cgcacatcgg cctctttccc gatgacggtg
     8101 tgtgtcacgc cttcgcgcgc atgcccgacc acctcggcac ctttctggcg ttgacgggtc
     8161 gcgccatcgg gccggccgat gcctatccgt tggggcttgc cacccacgtc atcccggccg
     8221 ctgacttcgg cgccatcgcc gcggcgatgg cggaggccga acccgtcgat caagtgctcg
     8281 atagccggca ccagccaccc cgccctggcg agctgacgga gggcgtgcgc gaggttgtgg
     8341 cgcggtgctt tgccgccgac acggtcgagg agatcatcgc ccgcctgcaa agccacggcg
     8401 ggccccgaca ggattgggca caagcggttg ccggcgagct gacgcgccgc tccccaacct
     8461 cgctcaagct gacccaccgc cacctcatcc aggcgcgcgg cctggatcta cgcgccacgc
     8521 tggtgcagga ctacagaatc gcccaccagt gcttggaggg cgaagatctc tacgaaggcg
     8581 tccgcgccgt ggtgatcgac aaggacgggg cgccgcgctg gcgcccggga cggcttgcgg
     8641 acgtgacgga ggcgctcctg gccccctact tcgcggcccc cgtggggggc gatctggccc
     8701 ttgccagccg ggcggaaatg caggcgctgc agagttaggg gggcgggcgc tttcgttaac
     8761 ccattgcttt aaggtcgaat tagagccttt cggctaccgt cggcggcaac cgggagcaac
     8821 accggcagcg tcagaacgga tcgagggctg ggaccatgaa ccttgttttg gaggccgcgc
     8881 gcccgcgcgc gccggtcggc gaagacacgt ttcgcagcga atatttgcgc gccttgcgct
     8941 tgatcgagcg cctgcatcgg ctgctgctcg acgtcatcaa ggacgaattc gatcgccgcg
//
LOCUS       DASTMT010000618         3923 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_5167594, whole genome shotgun sequence.
ACCESSION   DASTMT010000618 DASTMT010000000
VERSION     DASTMT010000618.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3923)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3923)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3923
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5167594"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..893)
                     /locus_tag="VFR00_14320"
     CDS             complement(<1..893)
                     /locus_tag="VFR00_14320"
                     /inference="COORDINATES: protein motif:HMM:NF004785.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEU0160493.1"
                     /translation="MRSKSSVILAAAGLLGLTAGLLAFGLGGASAKSGREGRQDGQLR
                     AAGKPVLAIVALRDQRISIYDAEGKILEAPVSTGSVGYETPAGIFSIVQKKELHSSNL
                     YEDGEMPFMQRLTWTGIALHAGVLPGRPASHGCIRLPMAFAQQLFDLTDLGMRVLIVR
                     DDMAPSDIAHPALFKSKLVGKEPAPAARSDRSLPSRATSEAKFGASEPNAGSARSWQT
                     LQSIGASRTTELEAANERLRDARAAATRAASDEAGSTRLLRAREVNLAKAETALADAE
                     RRLETSTEAPSNARAEAAKAKA"
     gene            complement(955..1806)
                     /locus_tag="VFR00_14325"
     CDS             complement(955..1806)
                     /locus_tag="VFR00_14325"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HEU0160494.1"
                     /translation="MLRAGVMLALLVSTTAATYAQKRPPEPASGSGRVFRDCPACPEM
                     VVVPAGEFMMGSPQSERGRSSNEGPQRTVAIKAFAMGKFEVTFAQWDACAAEGGCKLK
                     PSDENWGRGKRPVINVAWDDATQFVAWLSRKTGNAYRLPSEAEWEYAARGATRASDPH
                     PPFATGATINYKQANYDANFVYGDGKMGVFRQKTLDVGTFPANAFGLHDVHGNVWEWV
                     QDCYKDSYRGAPSDGSAVVLADCKLRILRGGSWNYFPQLLRSAYRYATAPDIRLDMVG
                     FRVARSL"
     gene            2366..>3923
                     /locus_tag="VFR00_14330"
     CDS             2366..>3923
                     /locus_tag="VFR00_14330"
                     /inference="COORDINATES: protein motif:HMM:NF024994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEU0160495.1"
                     /translation="MAIVGASPREGTLGNNVVTNLRRFGYPGRILPIHPSAPDVAGLP
                     AYRSFAQLPEIVECAVLAVPADKIVAALEDGAAHGLRAAVVFASGFAELGEDGRARQR
                     ELQALCARSGIVLCGPNCLGLVNVHQRVSLYSSGIPEGLEDGGLAVVSHSGSACIALS
                     TLGRFGLSFLVSVGNAAVLDLPDYLEYLANDRQTKVAALFIEELRQPAAFAGAIEAMH
                     TAGKPVIALKVGRSLKGAAASAAHTGSLAGSWDAYQAYFRRHGVIAVDDFDELAESVE
                     LALKLRSPPRGNGVAMIAVSGGEASLVADLAERARLELPELAPATVARLRTVLPSFAN
                     IGNPLDTTDRGVYDAQNVYNGSIRALAEDPAVSLVAVVQDCSPGLSARGANNYRRIAQ
                     TVADVARDVSKPVVFFNTAAGGLHPHVIEPLAGSPVAVLQGARASLLAISRLFAYTRY
                     APGAEPQVPAPAAHWRQRLASGQPFTERESKAFLAAHGIAVTREICAEDAAAAVRAAA
                     EIGYPVVLKIE"
BASE COUNT          625 a         1364 c         1226 g          708 t
ORIGIN      
        1 gccttggcct tcgcggcttc ggcccgtgcg ttgcttggcg cttcagtact tgtctcgagc
       61 cggcgttctg cgtccgccaa agccgtttcg gctttggcga gattgacctc ccgagcccgc
      121 aacagacggg tactcccggc ttcgtcggaa gcggcgcgtg tggcggccgc gcgcgcgtcg
      181 cgcaatcttt cgttggccgc ctcgagctct gtcgtgcggg aggcaccgat cgattgcagc
      241 gtctgccaag atcgtgcgct gcccgcgttc ggctcggaag ccccgaactt tgcctccgat
      301 gtggctctgc tcgggagcga acggtcgctg cgcgcggctg gggctggctc ctttccgacg
      361 agcttcgact tgaagagagc cggatgcgca atgtcgctgg gggccatatc gtcgcgcacg
      421 atcagcacgc gcatgcccaa atcggtcagg tcgaagagct gctgggcaaa tgccatgggg
      481 agccgaatgc agccatgcga ggccggacgc cccggcaaca cgccggcatg cagcgcgatg
      541 cccgtccagg tcaggcgctg catgaacggc atctcaccat cttcatagag attggaactg
      601 tgcagttcct tcttctgcac gatgctgaaa atgccggccg gcgtttcgta gccgaccgag
      661 ccggtcgaga ccggtgcttc caaaatcttg ccctcggcat cgtagatgga gatgcgctgg
      721 tcgcggagcg cgacgatggc cagcacgggc ttccctgcgg cgcgcagctg accgtcctgt
      781 cgcccctctc ggccgctttt ggcgctggcg ccgccaaggc cgaacgccag tagccccgcc
      841 gtgaggccca gcagccctgc ggcggccaag atgacgctcg acttcgatct catactgatc
      901 cggctcattg cgcagttgca ccctcgagcg ctgcctcaag cacaaaagtg atcctcagag
      961 cgatcttgcg acccgaaagc ccaccatgtc gagacggata tcgggggccg tagcatagcg
     1021 gtaggctgaa cgcagcagct ggggaaaata gttccacgag ccgccacgaa gaatgcgcag
     1081 cttgcagtcc gccaagacca ccgcggaccc gtcgctggga gcgccgcggt agctgtcctt
     1141 gtagcaatcc tggacccact cccagacgtt gccgtggaca tcgtgcaggc caaaggcatt
     1201 ggcgggaaag gttcccacgt ccagcgtctt ttgccgaaaa acccccattt tgccgtcgcc
     1261 gtagacaaaa ttggcgtcat agttcgcttg cttgtagttg atcgttgccc cggtcgcgaa
     1321 cggtggatgg gggtccgatg cccgcgtcgc cccgcgcgcg gcgtattccc actctgcctc
     1381 gctggggagc cgataggcgt tgccggtctt gcgcgaaagc caggccacaa attgtgtagc
     1441 atcgtcccag gcgacgttga tcaccggccg cttgccgcgc ccccagtttt cgtcacttgg
     1501 tttgagcttg cagccgccct ccgccgcaca cgcatcccac tgggcaaagg tcacctcgaa
     1561 cttgcccatg gcaaacgcct tgatcgcaac cgtgcgctgc gggccttcat tgctactgcg
     1621 gccgcgctcg ctctgcggtg atcccatcat gaactctccg gcgggcacga ccaccatttc
     1681 cgggcacgcc gggcaatcgc gaaagacccg tccgctgccg ctcgcaggct ctggcggtct
     1741 tttctgagcg taggtcgcag ccgtcgtact cacgagcaag gccagcatca caccagcacg
     1801 cagcattgac gtcccttgca tttccgtgac atcacccgtt gaccgagcag agagcggcgt
     1861 aggcggatga aaactccacg cccgcacgcg agaatcaatt ccctcgccgc gcgccatgtt
     1921 tgcacactcc gccgcgaggc gcatcgttgc gaatgcaccg gcgcccaatg tgacgcccaa
     1981 attcggccga aaagggccgg aactttgcgc gcactgagcg accacggcgt gcagtacgtc
     2041 tccggtttac cgccggcatc ggtggcgtgc caacgcgggc ccgcttgccg cggcctatcc
     2101 gctcggggcc agcaacgtgc caggccttag gatcggcgag gcatgcccgc gatgtcgggc
     2161 gcgacatcgc ggctgcggcc ttggccaacc ctgtcctgcc ggcgggctcg atccccgctt
     2221 gaagcatctg ccattcgtgc tcaccacgcc cgatcgtatt gacacctttg cagcgggcac
     2281 tagcctgcaa caaaccgacc gccgggcaca tgcaaagatg gccgtcccgc acttgcgtct
     2341 ttcgcctctg ttgacgccgc gctcagtggc aattgttggc gcctcaccgc gcgagggcac
     2401 gctcggcaac aacgttgtca cgaacctgcg ccgcttcggt taccctggcc gcattctccc
     2461 catccacccg agcgcgccgg acgtggcggg cctccccgcc taccgttcgt ttgcccagtt
     2521 gccggaaatc gtcgagtgtg cggtcctcgc ggtacccgcc gacaagatcg tggccgccct
     2581 cgaggacggt gccgcacacg gtctgcgggc cgcggtcgta ttcgcgagcg ggtttgccga
     2641 actcggcgaa gacggccgcg cgcggcaacg cgaattgcaa gcgctgtgcg cccgcagcgg
     2701 catcgtcttg tgcggcccga attgcctggg cctcgtcaac gtgcatcagc gcgtgtccct
     2761 ttatagctcg ggcatccccg aaggcctcga agacggcggc cttgccgtcg tctcccatag
     2821 cggctccgcc tgtattgcgc tctccacact ggggcgtttc ggtctctcct tcctcgtctc
     2881 cgtcggaaac gccgcggtgc tcgatttgcc ggactatctc gagtacctgg ccaatgacag
     2941 gcaaaccaag gtggcggcgc tcttcattga agagctgcgc cagcccgcgg cctttgcggg
     3001 cgccatcgag gccatgcaca cggcgggcaa gcccgtgatc gccttgaagg tcggccgctc
     3061 gctgaagggg gcggcggcct ccgcggcaca caccggctcg ctcgcgggca gctgggacgc
     3121 ctaccaagcc tatttccgcc ggcatggcgt catcgcggtc gacgacttcg atgaattggc
     3181 cgaatcggtg gaactcgcgc tcaagctccg ttccccgccg cggggcaacg gcgtggccat
     3241 gattgccgtc agcggcggtg aagcctcgct ggtcgcggac ctcgccgagc gcgcgcgcct
     3301 cgagctgccg gagctcgcgc cggcaacggt ggcgcggctg agaaccgtgc tgccgagctt
     3361 cgccaacatc ggcaacccgc tcgataccac cgaccgcggt gtctatgacg cccagaatgt
     3421 ttacaacggc tcgatccggg cgctggccga ggaccccgcg gtgtcgctcg tcgccgtggt
     3481 gcaggattgc tcgcccggtc tctccgcgcg gggcgccaac aattaccggc gcattgccca
     3541 gaccgtcgcc gacgtcgccc gtgatgtgtc caaaccggtc gtcttcttca ataccgcggc
     3601 gggtggcctg catccccacg tgatcgaacc cttggccggc agcccagtcg ccgtgctgca
     3661 gggtgcccgc gccagcctgc tggccatcag tcggctgttt gcctatacgc gttatgcacc
     3721 gggcgctgag ccgcaggtgc ctgcgcccgc cgcgcactgg cgccagcgcc tggcaagcgg
     3781 gcaacccttc accgagcgcg agtccaaggc cttccttgcg gcgcacggca tcgccgtgac
     3841 ccgtgaaatc tgcgccgagg acgccgcagc cgcggtcagg gccgccgccg agatcggcta
     3901 tccggttgtg ctcaaaatcg agt
//
LOCUS       DASTMT010000619         3666 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_5167683, whole genome shotgun sequence.
ACCESSION   DASTMT010000619 DASTMT010000000
VERSION     DASTMT010000619.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3666)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3666)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3666
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5167683"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..187
                     /locus_tag="VFR00_14335"
     CDS             <1..187
                     /locus_tag="VFR00_14335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018699042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEU0160496.1"
                     /translation="AYAIAIWAMTVAPIALVAALRETSVLFAALFGTLLLREPVLPVR
                     LVAALLVMAGALLLRFR"
     gene            complement(199..1023)
                     /locus_tag="VFR00_14340"
     CDS             complement(199..1023)
                     /locus_tag="VFR00_14340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HEU0160497.1"
                     /translation="MANFPTHIAVGTVVSGALATVTLAADVVAPENLVAVTLAGVLGS
                     VLPDIDLKDSRPSRAMFAGLGVFFAFAVLFSLQQKYSVVEMIILWLGTLVLVRYVAHA
                     FFHRLSAHRGIWHSILASIFCACATAAIYTTVLGRNEGVAWLAAGFLTIGYLTHLILD
                     EIYSVDVMDTRLKASFGTALKLIDPRHPGATAAMAVATVIAFLVAPPLKTFVDGVTSR
                     SLWSALQQRLLPHDNWFGVDWRQLARRSSPPATAKGANAIQTGSITPAPAVETPAK"
     gene            1283..2194
                     /locus_tag="VFR00_14345"
     CDS             1283..2194
                     /locus_tag="VFR00_14345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006609972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEU0160498.1"
                     /translation="MLAGPLQVADKLKVVAVGSIVVAAAVLALKFLAYWITGSVALYS
                     DALESIVNLITALVALYALHISAQPADRRHQFGHHKAEYFSAVLEGVLIVLAALLIVR
                     EAYDALVVPRRLNDPVLGLLINGAATVINAVWSYFLLTWGRRQRSPALVADGWHLLTD
                     VATSLGVLAGLFLATLTGWQVLDPVLAAVVAVNILWAGWRLMRDSISGLMDEAVTAEV
                     ARRIREVISNNAEGAIQAHDVKTRTAGRVTFIEFHLVVPGRMSVASSHQICDRIEDAL
                     ADAIPGAEVLIHVEPEHKAKTSGLPIA"
     gene            complement(2227..3060)
                     /locus_tag="VFR00_14350"
     CDS             complement(2227..3060)
                     /locus_tag="VFR00_14350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012333286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEU0160499.1"
                     /translation="MRTITLKTGEHVPILGLGTWRMGERKSARGAEINALRLALDLGV
                     RLIDTAEMYGEGAAEEIVGEAIAGKRDGLFLVSKVYPHNASRRGTIAACERSLRRLSV
                     DHLDLYLLHWRGSTPLAETVEALERLRGQGKIRRWGVSNFATEDMAELADLAAGPVCA
                     SNQVLYHLGSRGIEWDLLPYCRRAEIMVMAYSPLGQGPLLRKPPLARIAAKHGVTPAA
                     IALAFVLRQPGVVTIPKAVDPAHVKANLAALDVTLETEDLRALDAAFPPPKGTTPLEM
                     T"
     gene            3239..3619
                     /locus_tag="VFR00_14355"
     CDS             3239..3619
                     /locus_tag="VFR00_14355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011439730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEU0160500.1"
                     /translation="MDKALRDKGLAIRKAVLGEEYVNRAAAAADDFNGPFQEILNEYC
                     WGLVWSDERLPRKTRSMLNLVMLACLNRMHEWELHLRGALTNGVSKDEVQAIIHQIAI
                     YAGVPLGVECFRTARKVFAELDKG"
BASE COUNT          632 a         1144 c         1242 g          648 t
ORIGIN      
        1 ggcctacgcc atcgccatct gggccatgac ggtggccccg atcgcgctgg tggcggcgct
       61 ccgcgagacc agcgtcctat ttgctgccct gttcggcaca ctactgttgc gcgaacccgt
      121 cttgccggtg cgccttgtgg cggcgctcct ggtgatggcc ggcgcgctcc tgttgcgctt
      181 tcgctaggcg gcgggcggct atttggccgg tgtctcgacg gccggggcgg gcgtaatcga
      241 cccggtttgg atggcgttgg cacctttcgc cgtcgccggc ggcgacgacc gccgcgccag
      301 ctggcgccaa tcaacgccga accagttgtc atgcggcaac aagcgttgct gcagcgcgct
      361 ccacagggag cgcgaggtca ccccgtcgac gaaggttttc agcggcggcg ccaccaggaa
      421 ggcaatcacg gtggccacgg ccatagcagc ggtggcgccg ggatgacgtg gatcgatcag
      481 cttgagcgcc gtcccgaagg aggccttgag ccgggtatcc atcacgtcga cggaatagat
      541 ctcgtcgaga atgagatggg tcaggtagcc aatggtgaga aagcccgccg ccaaccaggc
      601 cacaccctcg ttgcgcccga gcaccgtcgt gtagatcgcg gcggtggcgc aggcgcaaaa
      661 gatgctggcc aggattgaat gccagatgcc gcggtgcgcg ctgagccgat ggaagaaggc
      721 gtgcgcgacg tagcgcacca ataccaaagt gccgagccag aggatgatca tttccacgac
      781 ggagtatttt tgttgcaagc tgaagagcac ggcaaaagcg aagaacacgc ccagcccggc
      841 gaacatggcc cggctcgggc gggagtcctt gaggtcgatg tcgggcagca ccgagcccag
      901 caccccagcg agcgtgacgg caaccaggtt ttccggcgcc accacgtcgg cggcgagtgt
      961 gacggtcgcc agcgcgccgc tgacgacggt gcccacggca atatgcgtcg ggaaattggc
     1021 catttcggca tgccttcttc cgggaaggga gacgcacgcg gacacatgac cgggcctgac
     1081 cagccggatc agagcttgct atgcatcaga ttcgctgcag gtgaactagc gatcgggcca
     1141 aaggagctgt gcattttgcc ggtcgccgcg gtcgttttca ccataccggt gaaggcgttg
     1201 gcggccatgc gacaatgaat tgcgctggca ttcgccggcc gttcgtgcca catcgagatc
     1261 gatagcgcag gctcgaaccc ggatgctggc aggacctctg caagtcgccg acaagctgaa
     1321 ggttgtcgcg gtcggctcga tcgtggtggc cgccgcggtg ctggccctca aatttctcgc
     1381 ctactggatc acgggcagcg tcgcgctcta ctccgacgcg ctggaaagca tcgtcaacct
     1441 gatcaccgcc ctggtggcgc tctatgccct gcacatctcc gcccagccgg ccgatcggcg
     1501 acaccagttc ggccatcaca aggcggagta cttttccgcg gttttggagg gcgtgctaat
     1561 cgtcctcgca gcccttttga tcgtgcggga agcctatgat gccctggttg tgccgcgccg
     1621 gctcaacgat cccgtgctgg ggctgcttat caacggggcg gccaccgtta tcaacgcggt
     1681 ttggtcctac tttttgctga cctggggccg ccggcagcgt tcgcccgcgc tggttgccga
     1741 tgggtggcac cttctcaccg acgtggcgac gtccttaggc gtgctcgccg gcctgttcct
     1801 ggcgacgctg acgggctggc aggtgctcga tccggtgctg gccgccgtcg tcgccgtcaa
     1861 catcctgtgg gcgggctggc gcctgatgcg cgattccatc agcggcctca tggatgaggc
     1921 ggtcacggcc gaggtggcgc ggcgcatccg cgaggtgatc tccaataacg cggaaggtgc
     1981 gatccaggcg cacgacgtca aaacccgcac ggccgggcgc gtgacgttca tcgagtttca
     2041 cctggtggtg cccggccgca tgtcagttgc ctcctcgcat cagatctgtg acaggatcga
     2101 ggatgcgctg gctgatgcca tcccgggtgc ggaagtgctg atccacgtcg aacccgagca
     2161 caaggccaag accagcggac tgccgattgc gtagggctgg cggctgggcg gccgcgcgcg
     2221 ggcccctcac gtcatctcca agggcgtggt gcctttgggc ggcgggaagg cggcatcgag
     2281 cgcgcgcaag tcctccgtct cgagcgtcac gtcgagggcc gcaagattgg ccttgacgtg
     2341 cgccgggtcg accgccttgg gaattgtcac cacccccggc tggcgcagga caaaggccag
     2401 cgcgatcgcc gccggcgtga ccccgtgctt ggcggcaatc ctcgccaggg gaggttttct
     2461 gagcaacggc ccctgaccca ggggtgagta ggccatcacc atgatctcgg cccgacggca
     2521 atagggcagg agatcccatt cgatgccgcg gctgccgaga tggtagagga cttgattgga
     2581 agcgcagacg ggcccggcgg caaggtcggc gagctcagcc atgtcctcgg tcgcgaaatt
     2641 gctgaccccc cagcgccgga tcttgccttg cccgcgcagg cgctccaaag cctcaaccgt
     2701 ctcggccagc ggcgtggagc cgcgccagtg caacaggtaa aggtcgaggt ggtcaacgct
     2761 cagccgccgc aaactcctct cgcaggcggc gatggtgcct cggcgtgagg cattgtgcgg
     2821 atagaccttg ctgaccagaa aaagcccatc gcgcttgccc gcaatcgcct cgccgacgat
     2881 ctcttcggcg gcgccctcac cgtacatttc tgcggtatcg atcaagcgca cgccaagatc
     2941 gagtgcaagc cgcagcgcat tgatttcggc gccgcgcgcg cttttgcgtt cgcccatgcg
     3001 ccaggtgccg agcccgagta tggggacgtg ctcgccggtc ttgagcgtga tcgtgcgcat
     3061 gttcgccatc ccttaatcct gggccgccgt tggggggcag cttggggctg cagcggcccg
     3121 tgacggcgct tgcgacgagc gcaagcgcca ccgctaggca acgcccttga ggccccgcgt
     3181 aaaggctgcc acaacgttgc gattggattg gcagtttgca agagcgatgg agagtgtggt
     3241 ggacaaggcg ttgcgagaca aggggctggc gatccgcaag gcggtgctgg gcgaagagta
     3301 cgtcaatcgc gccgcggcgg cggccgacga cttcaacggt ccgtttcagg agattttgaa
     3361 cgagtactgc tggggcctgg tgtggagcga cgagcgcttg ccgcgcaaga cgcgttccat
     3421 gctgaacctc gtcatgctgg cctgcctcaa tcgcatgcac gaatgggagc tgcatttgag
     3481 aggggcgcta acaaacggcg ttagcaagga tgaggttcag gccatcatcc accaaatcgc
     3541 catttatgcc ggggtgccgc tcggcgtgga atgcttccgc accgcgcgca aggtgtttgc
     3601 cgagctggac aagggctgag ggcgaatctg cgccctgcta ggcggcaaag cgcccgaaaa
     3661 tcgaga
//
LOCUS       DASTMT010000620         3473 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_4840935, whole genome shotgun sequence.
ACCESSION   DASTMT010000620 DASTMT010000000
VERSION     DASTMT010000620.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3473)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3473)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3473
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4840935"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..997)
                     /locus_tag="VFR00_14360"
     CDS             complement(<1..997)
                     /locus_tag="VFR00_14360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016845492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0160501.1"
                     /translation="MPSVVLRGLSKRFGGQTAVDDVSLRIEHGQLVCLLGPSGCGKTT
                     ILRLLAGFTEPTAGEIVIGERVVSAPNRVLPPERRNVSMVFQSYALWPHMTVAENVAY
                     GLQLRKLDRATIAAKLAAILATTQLAPLAERYPAELSGGQQQRVSLARALIVEPDMLL
                     LDEPLSNLDANLREEMRFEVRRLHQTYHYTTVYVTHDQSEAMTTADLIAVMNAGQIEQ
                     LGSPQEIYQQPRSEFVARFLGGSNILRGQALDAKSLAFAGSSIECHNALFVAGKPAAA
                     AVRQHDIRLLPAADGASGANQLQGTVVRNVFLGATRDYIVEVADGVPLRITAAPEA"
     gene            1463..2215
                     /locus_tag="VFR00_14365"
     CDS             1463..2215
                     /locus_tag="VFR00_14365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007592336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU0160502.1"
                     /translation="MAKNMIDLKGRAAVVTGGAQGFGKAISDRFLRSGARVAIWDRDT
                     ALAEKTAKALGAEVQAVACDVADPASVATALKATLEAFGRVDILINNAGIAGANAKTW
                     ETDVEEWRKVMRVNLDGPFICSRAVVPGMIQQNYGRIVTIASIAGKEGNPNAAHYSAS
                     KAGVIALTKSLGKELAGYDIAVNCVTPAAAKTAIFDQITQQHIDFMLSKIPRNRFVLV
                     EEVASMVAWMASEENSFTTGAVFDISGGRATY"
     gene            complement(2268..>3473)
                     /locus_tag="VFR00_14370"
     CDS             complement(2268..>3473)
                     /locus_tag="VFR00_14370"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0160503.1"
                     /translation="EGVISARKAQHQDIASIETPDPYTVVFKLSQVNMSMLLHFASPF
                     NCVYSAKKLKQNPKYPETEIMGTGPFRFVNYVKGSYWEGKRFEGYFRKGLPYLDGYKA
                     FFVKSSAVVPGILGGQFDTEFRSRNPSERDQLMSKMKDQLTLHEGRWVGSLMIVFNTK
                     RKPFDDIRVRQALTLAIDRWGGSTSLGKISLIKGVGGAFRPGAEWALPPEELEKIPGF
                     WRDIEKSRAEARRLLKEAGHPNLKVKLVNRTIDQPFIPAGIYAVDQWRRIGVETEHAP
                     VETKLWFAAMENGDFDAVVQNISDFADDPTAQFNTLLSKHTSGIAYSRHGDTKLDDLF
                     KLQSQIVDQRERMKVVHDFERYALTAAYNVPLLWYQRIVVNNKKVKGWELPPSHFVGQ
                     NLVEVWLDE"
BASE COUNT          615 a         1098 c         1137 g          623 t
ORIGIN      
        1 cggcctcggg cgccgccgtg atacgcaagg gcacgccgtc ggcaacctcg acaatatagt
       61 cacgcgtagc accgagaaag acattgcgca cgacggtgcc ctgcagctga ttggccccag
      121 aagctccgtc ggcggcgggc aacagccgga tatcgtgctg gcgcacggcc gcggcggcgg
      181 gcttacccgc aacgaatagg gcgttgtggc actcgatgct ggagccggcg aaggcgaggc
      241 tcttggcgtc gagagcttgg ccgcgaagga tgttgctgcc gccgaggaag cgcgccacga
      301 actccgagcg cggctgctgg taaatctctt gcggggagcc cagttgctcg atttggccgg
      361 cattcatcac ggcgatcagg tccgccgtgg tcatggcctc gctctggtcg tgggtgacgt
      421 agactgtggt gtaatgatag gtctggtgca gccggcgcac ctcgaagcgc atttcttcac
      481 gcaggttggc gtcaaggttc gacaagggct cgtcgagcag cagcatatcg ggctccacga
      541 tcaaggcccg ggccagcgag acgcgctgct gctggccgcc ggaaagctcg gcggggtagc
      601 gttcggccaa gggcgccaat tgtgtggtcg ccaggatcgc cgcgagcttg gccgcgatgg
      661 tcgcccgatc gagcttgcgc agctgcaagc cgtaggcgac attctcggcg accgtcatgt
      721 gcggccacag cgcgtagctt tgaaagacca tggagacgtt gcgccgctcg ggcggcagca
      781 cgcggttggg cgcggagacg acgcgctcgc cgatgacgat ttcccccgcc gtcggctccg
      841 tgaagcccgc cagcaggcgc aggatggtgg tcttgccgca gcccgaaggc cccaacagac
      901 acacgagctg cccgtgctcg atccgcagcg aaacgtcatc gaccgccgtc tgcccgccga
      961 agcgcttgct gagaccgcgc aaaacaaccg atggcacgca acctccctct ggcgcgctcg
     1021 ccacccgttc ccgtcggcgg caaaccgacg ttttttgcgc ccgcgcggct ctggctttag
     1081 catttctctt accattgcta tagcacccag cgcagcgatg ccatggcgcg cttgcgcttg
     1141 gcgcccgggc cggctggccg cagcgccggc cgccggcccc cgcggcacgc aacggccgcc
     1201 gagaagagac ccaagcgcac gccgccttgc ggtggtgatc gcccgcccga catccctctg
     1261 agagcttggc gccacctggc cgcgttcgga tgcaaccgga agtttcaacc tgaagtttca
     1321 tgggggcgtt caatgggcgc ccgagatgct tttagcgcgc gttggccccg gcgcattgcg
     1381 accaattctg tgctagccaa cgggctggcc cccggcggac gcatcgcgga cacgggctgc
     1441 aggcgaggag gcaagagcgc ccatggcgaa aaacatgatc gaccttaagg gtcgtgcagc
     1501 agtggttacg ggcggcgccc agggtttcgg caaggcgatc agcgatcgct ttttgcgctc
     1561 gggcgcgcgc gttgccatct gggaccgtga cacggcgctg gccgaaaaga cggccaaggc
     1621 gctcggcgcc gaggtgcagg cggtcgcctg cgatgtcgcc gatccggcct ccgtcgccac
     1681 cgcgctcaag gccacgctcg aggcgttcgg ccgcgtcgac atcctcatca acaatgcggg
     1741 catcgccggc gccaacgcca agacctggga gaccgacgtg gaggaatggc gcaaggtgat
     1801 gcgcgtcaat ctcgacgggc cgttcatctg ctcgcgcgcg gtggtgcccg gcatgatcca
     1861 gcaaaactat gggcgcatcg tcacgatcgc ctcgatcgca ggcaaggaag gcaacccgaa
     1921 tgccgcccac tattcggcct ccaaggcggg tgtgatcgct ctcaccaagt ccttgggcaa
     1981 ggagctcgcc ggctacgata tcgccgtcaa ctgcgtcacg cccgccgccg ccaagacggc
     2041 gattttcgat cagatcaccc agcaacacat cgactttatg ctgtcgaaga ttccacgcaa
     2101 ccgcttcgtg ctagtggagg aagtggcgtc catggtggct tggatggctt cggaggaaaa
     2161 ttccttcacc acgggcgccg tattcgatat ttccggcggt cgcgccacct attgagcgtt
     2221 cgccgcgccc ggcaaaagct gcaggcgcgg cggtggctgc tgcggcgcta ttcgtccagc
     2281 cagacctcga cgaggttctg tccgacgaag tgactgggag gcaattccca gcccttgacc
     2341 ttcttgttgt tgacgacgat gcgctggtac cacagcaggg gcacgttata ggctgcggtc
     2401 agggcgtagc gctcgaaatc gtgcaccacc ttcatgcgct cccgctgatc gacgatctgc
     2461 gactgcagct tgaacaggtc gtcgagcttg gtatcgccat ggcgggaata ggcgatgccg
     2521 gaggtgtgct tgctcaacag cgtgttgaac tgggccgtgg gatcgtcggc aaagtcggag
     2581 atgttctgca ccaccgcgtc aaagtcgccg ttttccatgg cggcaaacca aagcttggtt
     2641 tcgaccggcg cgtgctcggt ctccacgccg atgcggcgcc actggtcgac ggcataaata
     2701 ccggcgggaa tgaacggctg atcgatggtg cggttgacga gcttgacctt caggttcgga
     2761 tgcccggcct ccttgagcaa gcgccgggcc tcggcacgtg atttttcgat gtcgcgccag
     2821 aagcctggaa ttttctcgag ctcttcgggc ggcagcgccc actcggctcc cggccgaaag
     2881 gcaccgccga cgcccttgat cagggagatt ttgccgagcg acgtcgagcc accccaacga
     2941 tcaatcgcca gcgtcagcgc ctggcgcacg cggatatcgt cgaagggctt gcgcttggtg
     3001 ttgaacacga tcatgaggct gccgacccag cgaccctcat gcaacgtcag ctggtccttc
     3061 atcttggaca tgagctgatc gcgttcggag gggttgcggc tgcggaactc ggtatcgaac
     3121 tggccgccca ggatgccggg gacgacagcc gatgacttga cgaagaaagc cttgtagccg
     3181 tcgaggtagg gcagcccctt gcggaagtaa ccctcgaagc gcttgccctc ccagtaggag
     3241 cccttgacgt agttcacgaa gcggaacggc cccgtgccca tgatctcggt ctcgggatac
     3301 ttcgggttct gcttcagctt cttggcgctg tagacgcaat tgaacggcga tgcgaagtgc
     3361 agcagcatcg acatgttcac ctggctcagc ttgaacacga ccgtgtaggg gtccggcgtc
     3421 tcgatggagg cgatgtcctg gtgttgcgcc ttgcgcgcag agatgacgcc ctc
//
LOCUS       DASTMT010000621         3513 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_5170918, whole genome shotgun sequence.
ACCESSION   DASTMT010000621 DASTMT010000000
VERSION     DASTMT010000621.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3513)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3513)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3513
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5170918"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..379)
                     /locus_tag="VFR00_14375"
     CDS             complement(<1..379)
                     /locus_tag="VFR00_14375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolQ"
                     /protein_id="HEU0160504.1"
                     /translation="MNPLEILKPATGGGFSFLELFMTAHPVVKIVMLGLLAASVWAWA
                     IIVEKVFAFRRARREADRFEQMFWSGQSLEELYTTVAHGRTFSMASLFVAAMREWKRS
                     VEGNIRALGGIQMRVEKVMDVTIS"
     gene            complement(625..1029)
                     /locus_tag="VFR00_14380"
     CDS             complement(625..1029)
                     /locus_tag="VFR00_14380"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002711547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEU0160505.1"
                     /translation="MLTAEHLQNQLAPLFPGLMGIKLVEVSQDKIIATMRVRAELCTA
                     GGVCHGGAYMALADTLGAIGTVVNLPPNTRTTTIESKTNFLGAAAVDSEVTAESVPLH
                     RGRTTQVWQTSIKSAAGKLCAVVMQTQMVLPA"
     gene            complement(1093..1581)
                     /locus_tag="VFR00_14385"
     CDS             complement(1093..1581)
                     /locus_tag="VFR00_14385"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008194127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbgC/FadM family acyl-CoA thioesterase"
                     /protein_id="HEU0160506.1"
                     /translation="MSQPEGKGRWPDLSGRLVEDAAGRRHVLPVRVYFEDTDFSGLVY
                     HGSYVRWCERGRSDFLRLIGNDHRALLAGGGGREPAAFVVRRMGLEFLRPARIDEILE
                     VTTRVAASTAATLSLDQRISRANTLLFTAEVMVVLMSAGGKPLRLSAHLRDALRAKNA
                     PA"
     gene            complement(1568..2488)
                     /locus_tag="VFR00_14390"
     CDS             complement(1568..2488)
                     /locus_tag="VFR00_14390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018259977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEU0160507.1"
                     /translation="MVIRISRRRFLKTLGLSGAGTLALGSTYGFAIEPFWRLDVTRYA
                     VAPPGWPRGLKLSLAVIADLHAGGPAMPLGRIEEIVESTNALRPDAIVLLGDFAASHK
                     FKTRSVAPKDWAKALSELSAPLGVHAVLGNHDWWDDLGAQRRLAGPVQGRRVLENVGI
                     PVYENAVVRLSKAAQPFWLAGLGDQLAFIRRRRAGAWRKFQGVDDLKGTLAQVTDAAP
                     VILLAHEPDIFPHVPSRVALTLSGHTHGGQVRLFGYSPMVPSRYGNRFAYGHIVEDDR
                     HLVVSGGLGCSILPVRMGVPPEIVLVDVAA"
     gene            complement(2674..>3513)
                     /gene="ruvB"
                     /locus_tag="VFR00_14395"
     CDS             complement(2674..>3513)
                     /gene="ruvB"
                     /locus_tag="VFR00_14395"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEU0160508.1"
                     /translation="FRATSGPVIAKAGDLAALLTNLEERDVLFIDEIHRLSPAVEEIL
                     YPAMEDYQLDLIIGEGPAARSVKIDLAKFTLIGATTRTGLLTTPLRDRFGIPVRLNFY
                     SHPELTIIVSRGAGVLKVAMAPEGAAEIARRSRGTPRVAGRLLRRVRDFAAVDKAKII
                     TAEIADRALRQLEVDGEGLDALDHRYLLCIAKNYDGGPVGIETISAALSEPRDALEEI
                     VEPYLLQQGFIGRTPRGRVLTLRAYRHLGLSGPQRAATPQIGLFDERSEGDDGNGKAG
                     LGR"
BASE COUNT          642 a         1152 c         1154 g          565 t
ORIGIN      
        1 ggctgatggt gacatccatc actttttcga cgcgcatctg gatgccgccc agcgcccgga
       61 tgttgccctc caccgaccgc ttccattccc gcatggcggc cacaaacaac gaggccatcg
      121 agaaggtgcg accgtgagcg acggtggtgt agagctcttc cagcgactgt cccgaccaga
      181 acatttgctc gaatcgatcg gcctcgcgcc gggcgcggcg gaaggcgaac accttctcca
      241 cgatgatggc ccaggcccac accgaggcgg cgagcaggcc taacatgaca atcttgacga
      301 cgggatgcgc cgtcatgaac agctccaaaa aggagaaacc tccgcccgta gccggcttca
      361 agatctctag gggattcatg gtgttgtccg tctcacgtgg tcgctcaagg gtgtggctgg
      421 gtcgagcgct cagggatagc gacaaggagc atctgtggcc gcccgcaatg gcgacccccc
      481 gccctaccgc acatgtcctt tgcgtacccg tttcccgccc accggaccag ggccatactg
      541 gtcgccaatg tgtcgggaat tgggctcgct tagcccagcg cgcgccgtcc ggcaacacgc
      601 accacgcgac catccccgta ggagttacgc cggcagcacc atttgcgtct gcatgacgac
      661 ggcacacaac ttgccggccg cggatttgat gctggtctgc cagacctgtg tcgtgcggcc
      721 gcggtgcagc ggcaccgatt cggccgtcac ctcgctatcg acggcggccg cgccgagaaa
      781 attggttttc gattcgatgg tggtcgtgcg cgtattgggc ggcaggttga cgaccgtgcc
      841 aatggcgccc agcgtatcgg ccagcgccat gtaggccccg ccatggcaaa cgcctcccgc
      901 cgtgcacagc tccgcccgca cgcgcatggt ggcgatgatc ttgtcctggc tcacctccac
      961 gagcttgatg cccatcagcc ccggaaacag gggcgccagc tggttttgca ggtgctcggc
     1021 cgtcaacatt gagcggatct cccgttgccc gaggcggcgc ggtggctggc cggcgtcggc
     1081 cagggccgca gtctaagccg gcgcattttt ggcccgcaag gcgtcgcgca gatgggccga
     1141 cagccgcagc ggcttgccgc ccgccgacat caagaccacc atgacctcgg cggtaaacaa
     1201 aagcgtattg gcgcggctga tgcgctgatc gaggctgagg gtggccgccg tgctcgccgc
     1261 cacgcgcgtc gtgacctcga ggatttcatc gatccgcgcc ggccgcagga attcgaggcc
     1321 catgcggcgt accacgaagg cggcgggctc gcggccaccg ccgccggcca aaagcgcgcg
     1381 atggtcattg ccgatcaggc gcaaaaagtc cgagcggccg cgctcgcacc aacgcacgta
     1441 gctgccgtga tagacgaggc cggaaaaatc ggtgtcctcg aaatagaccc gaaccggcaa
     1501 cacgtggcgg cgtccagccg cgtcctcgac caggcggccg gaaagatcgg gccagcggcc
     1561 cttgccctca ggctgcgaca tcgaccagca cgatttccgg cggcaccccc atccgtaccg
     1621 gcagaattga gcagccaagg ccgccggaga cgacgagatg acgatcgtcc tccacgatat
     1681 ggccgtacgc aaatcgattg ccgtagcggg agggcaccat cggcgaatac ccgaacaaac
     1741 gcacctggcc gccatgcgtg tgcccggata acgtcagggc cacgcgcgag ggcacgtgcg
     1801 gaaagatatc gggttcgtgt gccaacagga tcacgggggc cgcatccgtc acctgcgcca
     1861 gcgtgccttt cagatcgtcg acgccctgga acttgcgcca ggcgccagca cgtcgcctgc
     1921 ggatgaaggc gagctggtcg ccgagcccgg ccagccaaaa cggctgggcg gctttgctca
     1981 gccgcaccac ggcattctcg tagacgggga tgccgacgtt ttcgagcacg cgccggcctt
     2041 gcacgggccc agccagtcgg cgctgggcgc ccaggtcatc ccaccaatcg tggttgccga
     2101 gcacggcgtg cacgccgagg ggggcgctga gctcggaaag cgccttggcc caatccttgg
     2161 gcgcaacgct gcgggtcttg aatttatggc tggcggcaaa atcgcccagc agcacgatgg
     2221 catcgggcct gagcgcattg gtgctctcaa cgatttcttc gatgcgtccc aagggcatcg
     2281 ccgggccgcc ggcgtgcaga tcggcaatga cggcaagcga tagtttgagg ccccgcggcc
     2341 agcccggcgg cgccacggca tagcgggtga catcgaggcg ccaaaagggc tcgatggcaa
     2401 atccgtaagt gctgccaagg gcgagcgtac cggcgccgga caagccaagc gtctttaaaa
     2461 acctgcggcg ggaaattctg atcacgatgg ctcgttccta ggcgtcctca ctggtgggct
     2521 cccggcgcga acgcgcaggg ctcggcaccg ccgtcggtgg tgtgacggca aagcatggaa
     2581 aagtttgggc atcggggacc cgagcggcca gcctgcgcac cgcacgcctg ccgcgaccgg
     2641 atgccgccgc gtgctctcga gcgggcctgg cgctcatcga cccaaaccgg ccttgccgtt
     2701 gccgtcatcc ccctccgatc tttcgtcgaa caggccgatt tgcggcgtgg cggcgcgctg
     2761 cggaccgctg agaccgaggt gtcgataggc acgcaaggtc aacacgcgcc cgcgcggcgt
     2821 gcgaccgata aagccttgct gcaatagata gggctcaacg atctcttcca gcgcatcgcg
     2881 cggctcggac agcgccgccg agatggtttc gatgccgacg ggcccaccat catagttttt
     2941 ggcgatgcag aggagatagc ggtgatccag ggcatcgagc ccttcgccat cgacctcgag
     3001 ctggcgcagc gcccggtcgg caatttccgc ggtgatgatc ttggctttgt cgacggcggc
     3061 gaagtcgcgc acgcgccgca ggaggcggcc ggcaacccgc ggcgttccac gcgagcggcg
     3121 cgcgatctcg gctgcccctt cgggagccat ggcgaccttc agcacgccgg ctccgcggct
     3181 gacgatgata gtgagttcgg ggtgactata aaaattcagc cgcacgggaa tgccaaagcg
     3241 atcgcgcaac ggcgtggtca gaagacccgt gcgcgtggtg gcgccgatca aggtaaactt
     3301 ggcgaggtcg attttgaccg agcgtgcggc gggaccctcg ccgatgatga gatcgagctg
     3361 atagtcctcc atggccgggt agagaatttc ctccacggcg ggactgagcc ggtggatctc
     3421 gtcgatgaac aggacgtcgc gttcctcgag gttggtcaac agcgccgcca ggtcgccggc
     3481 cttggcgatc acgggacccg aggtggcacg aaa
//
LOCUS       DASTMT010000622         3509 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_4515962, whole genome shotgun sequence.
ACCESSION   DASTMT010000622 DASTMT010000000
VERSION     DASTMT010000622.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3509
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4515962"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..775
                     /locus_tag="VFR00_14400"
     CDS             <1..775
                     /locus_tag="VFR00_14400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014491285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="benzoyl-CoA 2,3-epoxidase subunit BoxB"
                     /protein_id="HEU0160509.1"
                     /translation="AQSGFDPLSRTCRFMLTEDAHHMFVGETGVGRAIERTCQAMREA
                     GIEDANDIEAVRKLGVIDLPTMQKKANLHFSLSLDLFGSEVSTNAANYYTSGVKGRFQ
                     ETRIEDDHQLTKAVYPVSKLIDGTIKMVDEPALTAINMRLRDDYVNDCLKGVQRWNKV
                     IEKTGVNFRLKLPHVAFHRGIGEFANLHASPAGEVLSEQAWAKQKEQWLPSKADGDFI
                     AGLMTPCWERGKFASWIAPPRIGIDNKPGDFEYVKVDRA"
     gene            complement(900..1616)
                     /locus_tag="VFR00_14405"
     CDS             complement(900..1616)
                     /locus_tag="VFR00_14405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008971255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018784 - (S)-2-haloacid dehalogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase type II"
                     /protein_id="HEU0160510.1"
                     /translation="MTNRPDVEALLFDVFGTVVDWRTSIIEDLSGFGAERGLSLDWAA
                     FADEWRALYQPAMEEVRSGRRAWTILDVLHRESLEALAAKYGLAGLGEADKAHINKVW
                     HRLKPWPDAVAGLERLKKRYIIGTLSNGNVGLLTRMAKFAALPWDVVLGAETAHAYKP
                     LPQAYLANAALLNLQPAQVMLVAAHNGDLAAAASAGLSTAFVPRPSEHGAKQSRDLRP
                     ERDWDVIADSFVSLAAAMHC"
     gene            complement(1751..2845)
                     /gene="aroC"
                     /locus_tag="VFR00_14410"
     CDS             complement(1751..2845)
                     /gene="aroC"
                     /locus_tag="VFR00_14410"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018408904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEU0160511.1"
                     /translation="MSHNTFGHLFRVTTWGESHGPAIGCVVDGCPPLLPLEAAEVQHF
                     LDRRKPGQSRFTTQRREPDSVRILSGIFQDDAGRQVTTGTPICLLIDNVDQRGKDYAD
                     IKDKFRPGHADFTYWAKYGIRDFRGGGRSSARETAMRVAAGAIARKVIAGVTVRAALV
                     QVGAHTIDRQRWSWEEVDRNPFFSPDAGILDTWARHLDEVRKAGSSVGAIVEVVAEGV
                     PAGWGAPIYGKLDQDLAGAMMSINAVKGVEIGAGMRAAELSGEANADEIRMGNDGKPQ
                     FLSNNAGGILGGISTGQPIVCRFAVKPTSSILSPRRTIDRHGQETEISTKGRHDPCVG
                     IRAVPVAEAMMALVLADHCLRHRAQLGPQP"
     gene            3149..3436
                     /locus_tag="VFR00_14415"
     CDS             3149..3436
                     /locus_tag="VFR00_14415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0160512.1"
                     /translation="MSIRKTLIAVLAVAIAIAFAPLSDLQAATLTPGSGPGVDNPIVK
                     VAAKKKAAKKKAKKGKKKSAKKAKSKGPGSCGAYMYYSKKDRKCADARSKK"
BASE COUNT          639 a         1118 c         1152 g          600 t
ORIGIN      
        1 ggcgcaatcc ggcttcgatc cgctgtcgcg cacctgccgc ttcatgctga ccgaggacgc
       61 gcatcatatg tttgtcggcg agacgggcgt cgggcgggcg atcgagcgca cgtgccaggc
      121 catgcgggag gccggcatcg aggatgccaa cgacatcgag gccgtgcgca agctgggcgt
      181 gatcgatctg ccgaccatgc agaagaaggc caatctgcac ttctccctgt cgctcgatct
      241 gttcggctcg gaggtctcca cgaatgctgc gaactactac acctccggcg tcaagggtcg
      301 cttccaggag acccgcatcg aggacgacca ccagctcacc aaggccgtct accccgtcag
      361 taagctgatc gacggcacga tcaagatggt cgacgagccg gcgttgaccg ccatcaacat
      421 gcgcctgcgc gacgactatg tgaacgattg cctcaagggc gtgcagcgtt ggaataaggt
      481 gatcgaaaag acgggcgtca attttcgcct gaagctgcct cacgtcgctt ttcaccgcgg
      541 catcggcgaa ttcgccaatc tgcacgcctc cccggccggg gaagttctga gcgagcaggc
      601 atgggcaaaa caaaaagagc aatggttgcc gtcgaaagcc gatggcgact tcattgccgg
      661 gttgatgacg ccctgctggg agcgcggcaa atttgcctcc tggatcgccc cgccgcgcat
      721 cggcatcgac aacaagccgg gtgacttcga gtacgttaag gtcgatcgcg cctaaggttc
      781 accggcgcgg cctcgccgcg gttggcgcgc gcggcggcgg ccatacgcag gctcggtctc
      841 gtccgtgcgt gtatcccaag ctcggcgcgg cctgagcgcc aagcggcccg cagcctgcct
      901 cagcagtgca tggccgcggc gaggctcaca aagctgtcgg cgatgacgtc ccaatcccgt
      961 tcggggcgga ggtcgcggct ttgcttggct ccgtgttcgc tcgggcgcgg cacgaacgcg
     1021 gtgctgaggc cggcgctggc cgccgccgcc aagtcgccgt tgtgggcggc gaccagcatc
     1081 acctgcgccg gctgcaaatt gaggagcgcc gcattggcca ggtaggcctg cggcaacggc
     1141 ttataggcgt gcgcggtctc cgcgcccagc acgacatccc agggcagcgc ggcgaatttg
     1201 gccatccgcg tgagcagacc cacgttgccg ttcgacagcg taccgatgat atagcgcttc
     1261 tttagccgtt cgaggccggc aacggcgtcc ggccatggtt tcaggcggtg ccacaccttg
     1321 ttgatatgcg ccttgtcggc ctcgccaaga ccggccaggc cgtacttggc tgcgagcgcc
     1381 tcaaggcttt cgcggtgcaa gacatcgagg atggtccagg cgcgccggcc ggagcgaacc
     1441 tcctccatcg ccggctggta gagcgcgcgc cactcgtcgg caaaggcggc ccaatcgagg
     1501 ctgagcccgc gctcggcgcc gaacccgctc aaatcctcga tgatgctggt gcgccagtcg
     1561 accaccgtcc caaacacgtc gaacaacagg gcctcaacat ccgggcgatt ggtcatgatc
     1621 ggtcctcttg ctgggctaac ggcccaccgc cggcggcaag gggcctggcg cgcatcatgc
     1681 aaccgtggca ccgcgcaaac aagcaagcgg aaaggcagcg gttcgaccgt ggcgaagcgg
     1741 cacggacgcg ctagggctgc ggtcccagct gggccctgtg ccgcaggcag tggtcggcca
     1801 gcaccagcgc catcatggcc tcggccacgg gcacggctcg gatgccgacg cagggatcgt
     1861 gccgtccctt ggtcgagatc tccgtctctt gtccgtgccg gtcgattgtt cggcgcggcg
     1921 aaaggatgga ggaggtcggt ttcacggcaa agcggcagac gatcggctgg cccgtcgaga
     1981 tgccgccgag gatgccgccg gcattgttgg acaggaactg tggcttgccg tcattgccca
     2041 tccgaatttc gtcggcattg gcttcgccgc tcagttcggc ggcgcgcatg ccggcgccga
     2101 tctccacgcc cttcacggca ttgatgctca tcatggcgcc ggccagatct tgatcgagct
     2161 tgccgtagat cggggctccc cagcccgccg gcacgccctc cgccaccacc tcgacgatgg
     2221 cgcccaccga ggagccggct ttgcgcacct cgtccagatg gcgcgcccag gtgtcgagga
     2281 tgccggcatc gggcgagaag aacgggttgc ggtcgacctc ctcccaggac cagcgctggc
     2341 ggtcgatggt gtgagccccg acctgcacga gtgcggcgcg caccgtgacg ccggcgatga
     2401 ccttgcgggc aatggcgccg gccgcgacac gcatggccgt ttcgcgcgcg ctcgaccgcc
     2461 cgccgccgcg aaagtcgcga ataccgtact tggcccagta ggtaaagtcg gcgtggccgg
     2521 gacgaaactt gtccttgatg tccgcgtagt ccttgccgcg ctgatcgacg ttgtcgatca
     2581 agaggcaaat cggcgtgccg gtggtgacct gacggccggc gtcatcttgg aagatgcctg
     2641 acagaatgcg cacgctgtct ggctcgcgcc gctgggtggt aaagcgggat tgtccgggtt
     2701 tgcgccgatc gagaaagtgc tggacctcgg ccgcctcgag cgggagcaaa gggggacagc
     2761 cgtcgacgac gcagccgatc gcgggcccat ggctttcgcc ccaggtcgtc acgcgaaaca
     2821 gatgtccaaa agtgttgtgc gacatgggct cccggcttcg cagcgccgag ccggctcttt
     2881 aacgatagga tggcaagaat gcaacgcaag cgcggggttt ggacgcgctc tcgcaaatgc
     2941 gagaattgcc gaccgggcgg gcagcgcctt aaccctgccc gcgaatatcg tgtcctttgc
     3001 gccacttcga gaggttaatg cgcaccacaa ttcatggtgc gttcatttag cgctctctag
     3061 cctccctggg cgctgtcgcg cacgagcagg ggacaatcag gtttcctgcg gcgcgtcgac
     3121 gccgacatag aaatgggagg tacttggaat gagcatccgt aagaccctaa tcgccgtttt
     3181 ggccgtggcg atcgccattg ccttcgcacc gctgtcggac ctgcaagctg ccactctgac
     3241 gccaggttca ggccccggcg tcgacaatcc tatcgtcaag gtggccgcga agaagaaggc
     3301 cgcaaagaag aaggcgaaga agggcaagaa gaaatccgcc aagaaagcca agagcaaagg
     3361 tcccggctcg tgcggcgctt atatgtatta cagcaagaaa gaccgcaagt gcgccgacgc
     3421 gcggtcgaag aagtaaacgc aacctgtttg caggagtctg ggggcttcag gctcctgcaa
     3481 aatctatttt cgagggtgtg ccggcggcc
//
LOCUS       DASTMT010000623         3911 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_4846183, whole genome shotgun sequence.
ACCESSION   DASTMT010000623 DASTMT010000000
VERSION     DASTMT010000623.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3911)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3911)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3911
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4846183"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1012)
                     /locus_tag="VFR00_14420"
     CDS             complement(<1..1012)
                     /locus_tag="VFR00_14420"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HEU0160513.1"
                     /translation="MARQPVAQDVEAAPEADRLDGFPHPRETAALFGHAGAERMLAEA
                     FASGQMHHAWLLTGRLGIGKATLAYRLARHALAEPSERDQSGTTLEVAAETRAARQVT
                     ALSHPALLLLRRPYDARARRFTQAIPVDEVRRLKSFLGHTSGANAWRVVLIDSADELN
                     INAANAVLKSLEEPPGRTLFLLISAAPNGLLPTIRSRCRRLDLAALTAEPLRAAAMAA
                     LEARGLAAPDPDAWSRLEGVAQGSVRRLLQLVAAGGLELNARLEKLMGSLPRLDWQLA
                     HALTDEMGFGAPEQPYETFFELFLDLLARLVRTRTTGQGTAAEEALAARLIAPERLPA
                     WAE"
     gene            complement(1012..1668)
                     /gene="tmk"
                     /locus_tag="VFR00_14425"
     CDS             complement(1012..1668)
                     /gene="tmk"
                     /locus_tag="VFR00_14425"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012318491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEU0160514.1"
                     /translation="MAAGKFITFEGGEGVGKSTQAKLLVAALAQRGIDAILTREPGGS
                     PFAERVRAMLLDPATPPHGALAEALLFYAARADHLEATIAPQLAAGGWVVCDRFSDST
                     RVYQGSAGGLEPEVLEALDRLVVRGQHPDLTVVLDLDPREGLARVTRRRAPTASPDPA
                     DPFEGRDLAFHQRLRAGYLALAQAEPRRCVVIDATLTPERIAGAVWQVVSDRLLAGGS
                     "
     gene            complement(1738..2955)
                     /locus_tag="VFR00_14430"
     CDS             complement(1738..2955)
                     /locus_tag="VFR00_14430"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HEU0160515.1"
                     /translation="MISRSKRARVALAHVPQRAAYCLWLLLLFAAFALGGIGPADAQA
                     EFSVKAAQAILMDADTGAIMYQRNADEMMYPASMSKLMTLTVLFKALKAGDVKLSDEY
                     LMSENAWRKGGAPSGTSAMFVPLGTRASVEDLIKGITVQSGNDAAISIAENMAGSEDL
                     FAARMTEEARALGLSKSTFKNATGLYQPDHVTTARELALLARHIIQVYPEYYGYFAVK
                     EFPYRKHKFINRNPLLNLVAGIDGLKTGFIKESGYGMVASAKQDGRRLIAVLNGCATA
                     EERRDEARRLLEWGFKNFSEVKLFDAGEVIGHARVLGGERMFVPLTGNGDVNIWLARQ
                     PPNPRLKAQIIYNGPLKPPLRKGEQVARLRVTTPNESTNEVPLYVAEDVGRAGVMRRG
                     LDTLLYLATRWIP"
     gene            complement(3108..3722)
                     /locus_tag="VFR00_14435"
     CDS             complement(3108..3722)
                     /locus_tag="VFR00_14435"
                     /inference="COORDINATES: protein motif:HMM:TIGR00413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HEU0160516.1"
                     /translation="MRIAWRPSVVSCLALATAAGVAGACSSSGNEGRYGTASPRLVDG
                     KQPIPKGGGSYKLGAPYQIDGRWYVPQADATYDRTGLASFYAEDFHGRRTSNGEVFDM
                     WALTAAHPTLPLPSLAYVTNLENGRTLLLRLNDRGPYVNNRVIDLSRASARYLGVEGP
                     GLARVRVRYAGPAPLSGDDRREQRFLASQPWFQIRLARGPNDSR"
BASE COUNT          637 a         1314 c         1330 g          630 t
ORIGIN      
        1 actcggccca cgcgggcagg cgctcgggcg cgatcagccg cgccgccagg gcctcttccg
       61 ccgcagtacc ttgcccggtg gtgcgcgtgc gcaccagccg cgccagcaga tcgagaaaca
      121 actcgaaaaa cgtctcatag ggctgctcgg gcgcgccaaa tcccatctca tcggtgagcg
      181 cgtgcgcgag ctgccagtca agacgcggca gcgagcccat cagcttctcg aggcgcgcgt
      241 tgagctccag cccgcccgcg gccacgagtt gcagcagcct gcgcacactc ccctgcgcca
      301 ccccttcgag cctgctccag gcgtcggggt cgggcgcggc caggccgcgt gcctcgagcg
      361 cggccatcgc cgccgcgcgc aggggctcgg cggtcagtgc cgcgaggtcc aggcggcggc
      421 agcgcgagcg gatggtgggc aaaagtccgt tcggggctgc cgaaatcagc aagaacagcg
      481 tgcgcccggg cggctcctcc aacgacttca agaccgcgtt ggctgcattg atattgagct
      541 cgtcggcgga atcgatcagc accacgcgcc aggcattggc cccgctggtg tggcccagaa
      601 acgattttag gcggcgcacc tcgtccaccg gtatggcctg cgtaaagcgc cttgccctgg
      661 cgtcataggg tcgacgcagc aagagcagcg cgggatggga cagcgcggtc acctgacgcg
      721 cggcgcgcgt ctcggctgcg acctcgagcg tggtgccgct ctgatcgcgc tcgctgggct
      781 cggccagcgc atgccgcgcc aatcgataag ccaaggtcgc tttgccgatg cccaagcgtc
      841 ccgtcagcag ccaggcatgg tgcatctgcc cgctggcaaa ggcctctgcc agcatgcgct
      901 cggcgccggc gtgcccgaac aaggccgccg tttcgcgggg atgtggaaag ccatcgagcc
      961 ggtcggcctc gggggctgcc tcgacgtcct gtgccacggg ctggcgcgcc atcaggagcc
     1021 tcccgccagc aggcgatccg aaacaacctg ccagacggcg ccggcgatgc gctcgggtgt
     1081 gagagttgcg tcgatgacga cgcagcgccg tggctcggct tgcgcgagcg ccaagtagcc
     1141 ggcgcgcagg cgctggtgaa acgcgagatc gcgcccctcg aacggatcgg ctgggtctgg
     1201 gctggcagtg ggcgcgcgcc gtcgtgtgac gcgggcaaga ccctcgcggg gatccaggtc
     1261 gagcacgacg gtgaggtcgg ggtgctggcc cctcaccacc aggcggtcga gcgcctcgag
     1321 cacctccggc tccagtcccc cggcgctgcc ctggtagacc cgggtcgaat cggaaaagcg
     1381 gtcgcacacc acccagccac cggccgcgag ctgcggcgca atggtcgctt cgaggtggtc
     1441 ggcgcgggcg gcgtagaaca acaaggcttc ggcgagcgcc ccgtgcggcg gcgtggcggg
     1501 atcgaggagc attgctctga cccgctcggc gaacggcgag ccgccgggtt cgcgcgtcag
     1561 aatggcgtcg atcccgcgct gggcgagggc cgcgaccagg agcttggcct gcgtcgattt
     1621 accgacgcct tcgccgccct cgaaagtgat gaactttccc gctgccatcg cgcttgctgc
     1681 ccggcgctcg gtcagcccgg cggcgttatg cgttgccgtc gagcccggag gccctctcta
     1741 gggaatccag cgggtagcca aatacaggag cgtgtcaagt ccgcggcgca tcacgccggc
     1801 ccgcccgacg tcctcggcca cgtagagcgg tacctcgttg gtgctttcgt tgggcgtggt
     1861 cacgcgcaac ctggcgacct gttcaccctt gcgcaacggc ggcttcaggg ggccattgta
     1921 gatgatctgt gccttgagcc gcggattggg gggctggcgt gcgagccaga tattgacgtc
     1981 gccattgccc gtcagaggca caaacatgcg ctcgccgccc aagaccctgg cgtgaccaat
     2041 cacctcgccg gcatcgaaca acttgacctc ggaaaaattc ttgaagcccc attccagcaa
     2101 ccggcgcgcc tcatcgcgcc gttcctcggc ggtggcgcaa ccgttgagca ccgcgatcag
     2161 gcgccggccg tcctgcttgg cggaggcgac catgccgtag cccgactcct tgataaaacc
     2221 ggtcttgagc ccatcgatac ccgccacgag gttgaggagt gggttgcggt tgatgaactt
     2281 gtgcttgcgg taagggaact ccttgacggc gaaatagccg taatactcgg ggtagacttg
     2341 gatgatgtgg cgcgccaaca gcgcgagctc ccgcgcggtg gtgacgtggt cgggctggta
     2401 gaggccggtc gcgttcttga aggtggattt gctgaggcca agcgcgcggg cctcctcggt
     2461 catgcgcgcc gcaaacaggt cctccgaacc cgccatgttt tcggcaatcg agatcgccgc
     2521 atcattgccc gattgcacgg taatgccttt aatcaagtcc tccacggagg cgcgtgtgcc
     2581 gagcggcacg aacatggccg aggtgccgga cggtgcgcca cccttgcgcc atgcgttctc
     2641 gctcatcagg tactcatcgc tgagcttgac gtcgccggcc ttcagcgctt tgaagagcac
     2701 cgtcagcgtc atcagcttgc tcatgctggc cgggtacatc atttcgtcgg cgttgcgctg
     2761 gtacatgatg gcgccggtat cggcgtccat cagaatggcc tgcgcggcct tgacgctaaa
     2821 ctcggcctgc gcgtcggccg gaccgatccc gcccaaggcg aaggccgcga acagcagcag
     2881 cagccaaagg caatacgccg cccgttgcgg cacatgcgcc aaagcgaccc gcgcgcgctt
     2941 ggatctcgat atcatctgcc cgcacgtcat tcaccgtccc cgtgacattg tcgccgtgac
     3001 cgcatgcggc gcgttgggct tacaccccgc ccgttggccc accacgccaa cgtcctcagg
     3061 ggcccgcctg ctgccagccc ggtttggctg cagcatagtc tcgaatccta gcggctgtca
     3121 ttgggcccgc gcgccaatct gatctgaaac cagggttggg aggcgaggaa gcgctgctcc
     3181 cggcggtcat cgcccgacaa gggagcgggg ccggcatagc gcacccgcac ccgggcgagg
     3241 cccggcccct ccactccgag gtagcgcgcg ctcgcgcgtg acaggtcgat cacccggttg
     3301 ttgacgtagg gcccgcgatc gttgaggcgc agcaagagcg tgcgcccatt ctcgagattg
     3361 gtgacgtagg cgagcgacgg caatggcaag gtcggatggg cggcggtcag cgcccacatg
     3421 tcgaagactt cgccgttgga tgtgcgccgg ccgtgaaagt cctccgcgta gaaggaggca
     3481 agcccggtgc gatcgtaggt ggcatcggct tgcggcacgt accagcggcc atcgatttgg
     3541 taaggcgcgc ccagcttgta gctgccgcca cccttcggaa tgggttgctt gccgtccacg
     3601 aggcgcgggc tggccgtgcc gtagcgcccc tcattgcccg agctggaaca ggccccagcg
     3661 acgccggccg ccgtggccag cgcaaggcag gagacgaccg atggccgcca ggcgatacgc
     3721 atcacactaa aaccttccgc cggggagcag ccggagcgtt cgcggccccg ccgcgaaaaa
     3781 ccttgccaaa cccccgttag gaacgggtta actctggagc gatgggcggc gccacgtcgg
     3841 cgccttggtc acggccgggg gcagcctggg cgttatggct ttcggtgcag gcaggcgtat
     3901 ttgcgcacga g
//
LOCUS       DASTMT010000624         7338 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_6443163, whole genome shotgun sequence.
ACCESSION   DASTMT010000624 DASTMT010000000
VERSION     DASTMT010000624.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 7338)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7338)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7338
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6443163"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..310
                     /locus_tag="VFR00_14440"
     CDS             <1..310
                     /locus_tag="VFR00_14440"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009030296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEU0160517.1"
                     /translation="GVKDMVRISDARMSGTAYGTIVLHVAPEAAAGGPLALVRTGDRI
                     HLSVKERRLDLLVEAAELEKRRAHWRADTTTGLRGYDRLFREAVLQAPQGCDFQFLRR
                     "
     gene            complement(450..1511)
                     /locus_tag="VFR00_14445"
     CDS             complement(450..1511)
                     /locus_tag="VFR00_14445"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEU0160518.1"
                     /translation="MPPAGPTGRPPLGAPNQQALPKATAPGGASPDAGSARSLSPADA
                     RSGLPGPKGLDDVKKGREERVEAGGVKVIKEPGDRIIIKQDNRVIIQHDEAERFRKRP
                     DARTEKRADGVQVTFFDRPGGVRVVTEVDGNGRLLRRYRRGPDGREVHLIDNRRFYRN
                     VGIGVAVGVGVGIVALNLARPHITIPRERYIVEYDRARDADLDEVLLAAPVARLDRGY
                     SLEEIRYSHELREYTRRIDLDINFEFGAFDVTPDQYPKLERLARSMLRALDRDPDLVF
                     LIEGHTDAVGSDEDNLSLSDRRAASVATILSDQFGVPVENLVTQGYGEQYLKVETQGP
                     ERLNRRVAVRNIKGLMAER"
     gene            2330..3271
                     /locus_tag="VFR00_14450"
     CDS             2330..3271
                     /locus_tag="VFR00_14450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019995477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HEU0160519.1"
                     /translation="MQAVEIVEVAPRDGFQAVKPFIPTERKIAIIEELAACGVRRLEI
                     GSFVSPKAVPQLADTGEVLARAKLPANLRLQALVPNAKGLELARKAGVAEVAWVISVS
                     ESHNRANVNRSVEESFRAFEEAWGALGPDRPKLRFGLSTCFDCPWEGRVPEAAVIRCV
                     ERAIAAAPELEIGICDTTGRAAPDHVGSLFARLIADYASQAVTFAFHGHDTYGLGVAN
                     AIEAFRQGVRVIDGAAGGLGGCPFAPGAAGNTASEDLVFAFEHMGIKTGIQFDKLLDA
                     ALHTAAVAPEQAGGKLRSVPRARALSGFAAASRGVAA"
     gene            3321..4955
                     /locus_tag="VFR00_14455"
     CDS             3321..4955
                     /locus_tag="VFR00_14455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008552634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEU0160520.1"
                     /translation="MHIAGASYDEVMRSFRWELPHHFNIAEAICEQHARRAPDATALI
                     YEAADGSVRHWSFAKISAAAGRCANVLAAFGVGRGTVVGIHLAQGPETLIAHVAIQKL
                     GAIALPLFNLFGPDAIGYRLADSGARVLITTPEALARTEQVVRANDTPLKVITVGARF
                     PGDAQEFWHLLAAAADMAATAATGAEDPALLIYTSGTTGNPKGALHAQRVLIGHLPGV
                     VMPHDFFPQVGDRFWTPADWAWVGGLLDVLFASLYFGVPVVASQRAKFDPEWAFAFLA
                     RHGVRNTFMPPTALRLMQQVPEPRARHAFQLRSVGTGGERLGEAMIAWGRDTFDLELA
                     EFFGQTEVNLVIGNAPRLFPVRAGSMGRAVPGHVVEIVDADGNLLPVGKAGIVAVRKP
                     DPVMFLEYWRKPEATAEKFRQGWCLLGDVAVKDEAGYFWFQGRDDDIISSAGYRIGPG
                     EVEDCLAKHPAVALAGVIGSPDPIRGEVVAAFIVPRTGFAPTRELAAEIQSFVKARLA
                     AHEYPRKVHFLESMPMTVTGKIRRHDLRELDRQRSG"
     gene            complement(5071..5874)
                     /locus_tag="VFR00_14460"
     CDS             complement(5071..5874)
                     /locus_tag="VFR00_14460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018689303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acyl homoserine lactonase family protein"
                     /protein_id="HEU0160521.1"
                     /translation="MTTETYEIFAIKYGEFSKRKRYESFISADDHDGPHPITYFVWLI
                     RNDRRSILVDSGFDPAEGAKRGRQIDRLPAAALELIGLSASRIEDLIVTHLHYDHAGT
                     LGAFPAARFHLQTAEMAYATGPCMCHAHLRYPFTADHVCEMVKNVYSGRVQFHDGDAE
                     VAPGVSVHKIGGHSRGLQCVRVMTASGPVVLASDSSHFYENFETGKVFPIVVDVEDTL
                     KGYARLVELAASPKRVVPGHDPLVLARYPSLNRETNGIVHRLDVARSDG"
     gene            6044..>7338
                     /locus_tag="VFR00_14465"
     CDS             6044..>7338
                     /locus_tag="VFR00_14465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-CoA decarboxylase"
                     /protein_id="HEU0160522.1"
                     /translation="MNVSFLQELLNTIADQGRQLLPRSLAWLDRTEDIGALAQALVSG
                     RGEASGVAIASAILARYRDLDADDRRAFLMFLARTMEPDAESVERAANDYLRTPGAAS
                     VAALQRAVESPRLEFFRRLNLAPGATAAIVAMRRDLIEGRDAALAPVNRDLMHLLSSW
                     FNRGFLVLRRIDWQTPAAILDKIIAYEAVHEIQGWDDLRRRLDPVDRRCFAFFHPSLV
                     DEPLIFVEVALSDAIPDNIQSLLQEGAKADSAQEPTTAVFYSISNCQEGLRGISFGNF
                     LIKQVVEDLVKERPSLKTFVTLSPVPDFADWLAEVLHDDPSGLVGAAERAQLAALDDP
                     NWILGPEVDALRPLLLRLAAHYFLRAKLENGRPADPVARFHLGNGARLERINWLGDTS
                     AKGMREAHGLMCNYRYELRDIEKNHEAYENEAVVAASRQV"
BASE COUNT         1109 a         2349 c         2545 g         1335 t
ORIGIN      
        1 cggggtcaag gacatggtgc gcatctccga tgcgcgcatg tctggcacgg cctatggcac
       61 catcgtcctg cacgtggcgc cggaggcggc ggcgggtggt ccgctggcgc tggtgcggac
      121 gggcgatagg atccacctca gcgtcaagga gcgccgcctc gatctcctgg tcgaggccgc
      181 cgagctcgag aagcggcggg cgcattggcg cgccgatacg acgacgggcc tgcgcggcta
      241 cgaccggctg ttccgggagg cggtgctgca ggcgccccag ggttgcgatt tccaatttct
      301 gcggcggtga tggccgctgc caaagccgca gccgtcgcgc gccgaagaga gccgcaacgc
      361 aacggcgtta gcccgccgca cggctggaac ggcggcgagc ccggggcggc ggtttgtcgc
      421 cgacaccccg gggcagcggc gtccttgcct cagcgctccg ccatcagtcc tttgatgttg
      481 cgcacggcga cgcgacggtt gaggcgctcg gggccttgcg tctcgacctt gagatactgc
      541 tcgccgtagc cctgggtaac gagattttcc accggcaccc cgaactggtc ggagaggatc
      601 gtcgccaccg aggcggcgcg ccggtcggag aggctcaggt tgtcttcgtc ggagccgacg
      661 gcgtcggtgt ggccttcgat caaaaacacc agatcggggt cacggtcgag cgcacgcagc
      721 atcgagcggg cgaggcgctc gagttttgga tattgatcgg gggtgacgtc gaaggcgccg
      781 aactcgaagt tgatgtcgag atcgatgcgg cgcgtgtact cgcgcagctc gtggctatag
      841 cggatttctt ccaaggaata gccgcggtcg agccgcgcta cgggcgctgc cagcagcacc
      901 tcgtcgaggt ctgcgtcgcg ggcgcgatcg tactcgacga tgtagcgctc gcgcggaatg
      961 gtgatgtgcg ggcgcgctag gttgagggcg acgatgccca cgccgacgcc gaccgccaca
     1021 ccgatcccga cgttcctgta gaagcggcga ttgtcgatca ggtgcacctc gcgaccgtcc
     1081 gggccgcggc ggtagcggcg cagcagtcgg ccgttgccgt cgacctcggt gaccacgcgt
     1141 acaccgccgg ggcgatcgaa gaaggtgacc tggacgccgt cggcgcgctt ctcggtgcgt
     1201 gcatcgggcc gtttgcgaaa acgctctgcc tcgtcatgct gaatgatgac gcgattgtcc
     1261 tgtttgatga tgatgcgatc gccaggctct ttgatcacct tgacgccgcc ggcttcgacg
     1321 cgctcctcgc gtcccttctt cacgtcatcc agacccttgg gtcccggtag accgctgcgg
     1381 gcatcggcag ggctgagcga ccgcgcgctg ccggcgtcgg gtgaggcgcc gcccggtgcc
     1441 gtggccttcg gcagtgcctg ctgattgggc gcgcccagtg ggggccgacc ggtggggcca
     1501 gcgggtggca tcggcggccc gccgggagga agggtggcgg cgcccggggc accgggactg
     1561 gtcggcgcgc cggtgcgcgg cgtgccggcg gggggttgtt gcccgggcag ggcaggagag
     1621 acggctcccg gcgtgccggg gccgctcttt tccgtcggca gtgcgccttg cccgcgtttg
     1681 cgctcgggga aaggcggcac ttcgcccctt ccttcgcccc ttccttcgcc cctgggcgcg
     1741 ccttcgccgc gcgagggcgc tgcatctggc gccttgaacg tgggcgatgc tgcaccgggg
     1801 cgcttttccg ccgggggcgg tagtggcggg gccggttggg ctctgggcgg cggcggcgcc
     1861 tttggcggcg gcggtggtgg tgccttgggt gcgggcggtg gtgcctttgg cggtgggccg
     1921 ggcggcgctg ccttcggcgg tggtgccttt ttcgccggcg gctgaccctt gtctttcttt
     1981 tggtcctcgc tctgttgcgc ctgggcgcgc tcgatcagcc cggcgtgctc tgccgccaag
     2041 gtgcccaagg tcgcagaaac cgatgccgtc acgagcaatg aggttgaaag acggaaacgc
     2101 gggcggaatg tcattgtggc tcccaacagt tgcgagcacg caagcgggtg cctgatctgg
     2161 tggcacaccg gcgcaggggg cgagcgcgtg cgttcaagtt tgcttgagca acgccatcgg
     2221 cgcactttga ggcggcagcg aggcgaatgt ttcgtatact gcccgcagcg gcggtcaaaa
     2281 tctcaagtgc ccgcccggca aaaaaccagc gcagcttatg agaatgtcga tgcaagctgt
     2341 tgaaatcgtt gaggtagctc cgcgggacgg gtttcaggct gtcaagccct tcatccccac
     2401 cgaacgcaag attgccatca tcgaagagct tgccgcctgc ggcgtgcggc ggctggagat
     2461 cggctccttt gtcagtccca aggccgtgcc acagctggcc gacacgggcg aggttttggc
     2521 gcgagccaag ttgccggcaa acctgcgcct gcaggcgctg gtccccaatg ccaaggggct
     2581 cgagcttgcc cgcaaagccg gggttgccga agtggcctgg gtgatctcgg tgtcggagag
     2641 ccacaatcgc gccaacgtca accgcagcgt tgaggagagc ttccgggcct tcgaagaggc
     2701 ctggggtgcg ttagggcccg acaggcccaa gttgcgcttc ggcttgtcga cctgcttcga
     2761 ttgcccctgg gaagggcgcg tccccgaggc ggccgtgatc cgctgcgtcg agcgcgccat
     2821 tgccgcggcc cccgagctcg agatcggcat ttgcgatacg accggccgcg ccgcgccgga
     2881 ccatgtgggg tcgttgtttg cgcgcctcat cgccgactat gccagccaag ccgtgacctt
     2941 cgccttccac ggccatgaca cctacgggct gggcgtcgcc aatgccatcg aagcctttcg
     3001 ccagggggtg cgcgtcatcg atggtgcggc cggcggtctc ggcggctgcc cgtttgcgcc
     3061 cggcgccgcc ggcaatacgg ccagcgagga tcttgtgttc gccttcgaac acatggggat
     3121 taagacgggc atccagttcg acaagctgct cgatgcagcc ctgcacacgg ccgcggttgc
     3181 cccggagcag gctggtggca agctgcgctc ggtgccgcgc gcccgcgccc tgtcgggctt
     3241 tgccgccgcc agccgtgggg tggcggccta agcattccgc tcaactcaga aggcccaagc
     3301 ggtaacgacg cgagatcagc atgcacatcg ccggcgcctc ctacgacgag gttatgcgct
     3361 cgtttcgctg ggagctgccg caccacttca acatcgccga ggccatttgc gagcagcacg
     3421 cccgacgcgc gcccgacgcc accgccctca tctatgaagc ggcggacggc tcggtacgac
     3481 attggagctt tgccaaaatc agcgccgctg ccggccggtg cgcgaacgtg ctggcggcct
     3541 ttggcgttgg tcgcggcacg gtggttggca tccacctggc gcagggtccg gaaaccctca
     3601 tcgcccacgt ggccattcag aagctcggcg ccattgcctt gccgctgttc aacctctttg
     3661 gcccggacgc catcggctat cgcttggccg atagcggcgc acgcgtcctg atcacgacgc
     3721 ccgaggccct ggcgcggacc gaacaggtgg tgcgggcgaa cgacacgccc ctcaaggtca
     3781 tcacggtggg cgctcgcttc cccggtgatg cccaagaatt ttggcatcta ttggcggccg
     3841 ccgccgacat ggcggcaacc gcggcgacgg gcgcggagga tccggccctg ttgatctaca
     3901 cgtcgggcac caccggcaac ccgaagggcg ccctgcacgc tcagcgcgtt ttgatcgggc
     3961 atctgccggg tgttgtcatg ccgcacgact tttttccgca agtcggcgat cgcttctgga
     4021 cgcccgccga ctgggcgtgg gtgggtgggc tgctcgacgt gctgtttgcg tccctctatt
     4081 tcggcgtgcc cgtcgtggcc tcccagcgcg ccaagttcga ccccgaatgg gcctttgcct
     4141 tcctggcgcg ccacggcgtt cgcaacacct tcatgccgcc cacggcgctg cgtttgatgc
     4201 agcaggtacc cgagccgcgc gcgcggcatg cctttcagct gcgctcggtc ggcacgggtg
     4261 gcgagcggtt gggtgaggcc atgatcgctt ggggccgcga caccttcgat ctcgagcttg
     4321 ccgagttctt cggccagacc gaggtcaatt tggtgatagg gaacgcccca cgtttatttc
     4381 cggtgcgggc tggctcgatg gggcgggcgg tgcctggtca tgtcgtggag atcgtcgacg
     4441 ccgacggcaa tctgctgccc gtcggcaagg ccgggatcgt tgccgtgcgc aagcccgatc
     4501 cggtgatgtt tctggagtac tggcgcaagc ccgaggcaac ggcggagaag tttcgccagg
     4561 gctggtgcct attgggtgac gtggcggtca aggacgaggc gggctatttc tggttccaag
     4621 gtcgcgatga cgacatcatc agctcggccg gataccgtat cggccccggc gaggtggaag
     4681 actgtttggc caagcatccc gccgtggcgt tggccggcgt gatcggctcg ccggatccca
     4741 tccgtggcga ggtggtggca gccttcatcg tgccaaggac aggttttgcg ccgacgcgcg
     4801 agctcgccgc cgaaatccaa agcttcgtca aagcacgcct ggcggcgcac gaatatcccc
     4861 gcaaagtgca ttttctcgag agcatgccga tgacggtgac gggcaagatc cgccgccacg
     4921 atttgcgcga gctcgatcgc cagcgctccg gctgatgcgg gccgccgcgg ggctggcggc
     4981 gcgcgggcaa tggccgcagc cgcggcgcgg caagagctgc tggggaggcg cgccgcggcg
     5041 cgccgcgcct catgggcggt gcagcgtcgg ctagccgtcg ctgcgcgcta cgtcgaggcg
     5101 gtggacgatg ccgttcgtct cgcgattgag gctcgggtat cttgccagca ctaggggatc
     5161 gtggccgggc accacgcgct ttggcgaggc cgccagctcg acgagccgcg cataaccctt
     5221 gagcgtgtcc tccacatcga ccacgatcgg gaacaccttg ccggtctcga aattctcgta
     5281 gaaatgcgag ctgtcggagg caagcacgac cggccccgag gccgtcatga cgcgcacgca
     5341 ctggaggccg cggctgtgtc cgccgatctt gtgcacgctc acccccggcg cgacctcggc
     5401 gtcgccgtcg tggaattgaa cccgccccga gtagacgttc ttcaccattt cacagacatg
     5461 gtcggccgtg aacggatagc gcaagtgcgc gtgacacatg cacgggccgg tggcgtaggc
     5521 catctcggcg gtctgcaggt ggaagcgggc cgcgggaaac gcaccgagcg tacccgcatg
     5581 gtcatagtgc agatgggtga cgatcagatc ctcgatccgg ctggcggaaa gaccgatcag
     5641 ctcgagggcg gccgccggca gccggtcgat ttggcggccg cgcttggcac cctcggccgg
     5701 atcgaacccg ctgtcgacga ggatgctgcg tctgtcgttt cggatcagcc aaacgaagta
     5761 ggtaatggga tggggcccat cgtggtcgtc ggcggagatg aagctctcgt agcgtttgcg
     5821 tttgctgaac tcgccgtact tgatggcgaa aatttcatag gtttcggtcg tcacgggcga
     5881 tgctccctgc caaatgttct cgggcggtgt agaatgccgc ccaagcgcgg cggtgtcacg
     5941 aaggcatcgc cagcgtctct atcctgcccg ggcgaggttg gatggccgca ccgcaatcgc
     6001 tacatgatgc tgtcctaggc ggccaacgcg aaagaatgac cccttgaacg tttccttcct
     6061 gcaagaactg ttgaatacga tcgccgacca gggccggcag ctgctgccgc ggtccctggc
     6121 ctggctcgac cgcaccgagg atatcggcgc attggcgcag gctttggtgt ccgggcgcgg
     6181 ggaggcgtcc ggcgtggcga tcgccagcgc gattctggcg cgctatcgcg atctcgacgc
     6241 cgatgaccgg cgcgcctttc tcatgtttct cgcgcgcacc atggagcccg atgcggagag
     6301 cgtggaacgt gcggccaatg actatcttcg cacgcccggt gcggccagcg tcgccgcctt
     6361 gcaacgtgcc gtggagagcc cgcgcctgga gttctttcgc cgcctcaacc tggcgcccgg
     6421 cgcgacggcg gcgatcgtgg cgatgcgccg cgatctgatc gagggccgtg atgccgcctt
     6481 ggcgcccgtc aatcgcgact tgatgcacct cctctcctcc tggttcaatc gcggcttctt
     6541 ggtgttgcgg cgcatcgact ggcaaacgcc ggcggcgatc ctcgacaaga tcattgccta
     6601 cgaggcggta cacgaaatcc agggttggga cgatttgcgg cgccggctcg atcccgtcga
     6661 tcggcgttgc tttgccttct ttcatccctc gctcgtggac gagccgctga tcttcgtcga
     6721 ggtggcattg agcgatgcca ttcccgacaa cattcaatcg ttgctgcagg aaggggccaa
     6781 agccgactcc gcgcaagagc ccaccacggc ggtgttctac tccatatcca actgccagga
     6841 aggcctgcgc ggcatctcgt tcgggaactt tttgatcaag caagtcgttg aagacctcgt
     6901 gaaggagcgc ccgagtctca agaccttcgt gacgctgtct ccggtaccgg attttgccga
     6961 ctggctggcc gaagtgttgc acgacgatcc gtccgggttg gtcggcgcag ccgaacgcgc
     7021 acagctggcg gcgctcgatg accccaactg gattttaggg cccgaggtcg atgccttgcg
     7081 gccgttgctg ctgcggctgg ccgcgcacta ttttttgcgt gccaagctgg agaatggacg
     7141 ccccgccgat cccgttgccc gtttccacct cggcaacggc gcccggctcg agcgcatcaa
     7201 ctggctgggc gatacctcgg ccaagggaat gcgcgaggca cacggcctga tgtgcaatta
     7261 tcgctacgag ctgcgcgaca tcgagaaaaa tcacgaggcc tacgaaaacg aggccgtggt
     7321 ggccgcctca cgccaggt
//
LOCUS       DASTMT010000625         6533 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_5782977, whole genome shotgun sequence.
ACCESSION   DASTMT010000625 DASTMT010000000
VERSION     DASTMT010000625.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 6533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6533
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5782977"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            6..632
                     /locus_tag="VFR00_14470"
     CDS             6..632
                     /locus_tag="VFR00_14470"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018387265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEU0160523.1"
                     /translation="MAAEPLDAFYPIVPDVAWLRRLVPLGIRTVQLRLKDAEPERVRR
                     EIAESLEICARHNCQLIVNDYWREALELGADFIHLGQEDFAAADIGAIAAKSLRLGIS
                     THSEAELAIALSARPTYVALGPIFETKLKVMQWAPQGLQRIRQWKDRIGPLPLVAIGG
                     ITPERAGDVWAAGADSVAVITDFFTHSNPTARVREWIALARAEGPRRA"
     gene            726..1787
                     /locus_tag="VFR00_14475"
     CDS             726..1787
                     /locus_tag="VFR00_14475"
                     /inference="COORDINATES: protein motif:HMM:NF017085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HEU0160524.1"
                     /translation="MAYRLKLKTTLAHEVRRIALEQIEIARGALARPEDRAAAVHDAR
                     RSLKRLRAMLRLVRPALGETWFKQSNRQLANLGQRLALSRDLDVMIVTLSKLESGAGG
                     LPKPVADRLRSAVARSRNKADSAGTPGADRSLARALQRAQTLFASERLRGVGLEHVAA
                     GLEQSYRKARRTFREAYAAASDEAFHNWRKATQAHWRHMQLLGRSWPESIGARAAEAK
                     ELSRLLGEDHDLSVLIGFAQSPGCGGLARADLETLTTACLKGQRQIRDVARFHGERLF
                     ADRPRHLKKRILRYWKAAEQLSTLLPPDGEATPQNPIPARPRPPRATQVERVGVRAAA
                     GKGAAQRRSRRGQAVDENP"
     gene            2023..3345
                     /locus_tag="VFR00_14480"
     CDS             2023..3345
                     /locus_tag="VFR00_14480"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012334903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HEU0160525.1"
                     /translation="MRIAMIGTGYVGLVSGACLADFGHILTCVDSDSSKIAALKRGDI
                     PIFEPGLADLVARNVREGRLAFSTELAPAVKTAEAIFIAVGTPSRRGDGHADLSYVFQ
                     AAREIATALDDHKVIATKSTVPVGTGDEVARIIAEARPNLTFSVVSNPEFLREGAAIA
                     DFKRPDRIVIGAEDERGRKLMRDVYRPLYLNQAPMLFTDRRTAELIKYAANAFLATKI
                     TFINEIADLCERAGANVQDVARGIGLDNRIGAKFLNAGPGFGGSCFPKDTLALMKTGH
                     DFGVPSRIVETVIAVNDQRKRAMGRKVIDVCGGNVRGRTIAVLGLTFKPNTDDMRDAP
                     SIAVITALQDAGANIRAYDPAGMKQAKSVLDNVEYSSDPYACAEGADALVILTEWDEF
                     RALDVDRVRAKLKRPILVDLRNIYRPEEMARQDFTYVSVGRPTARKEA"
     gene            3404..4447
                     /gene="hemH"
                     /locus_tag="VFR00_14485"
     CDS             3404..4447
                     /gene="hemH"
                     /locus_tag="VFR00_14485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HEU0160526.1"
                     /translation="MNAQDTPSSVAVPEPKSSAGRGRIGVLLLNLGTPDATSYWPMRR
                     YLREFLSDRRVIDVPRLKWWPILNLVILTVRPGPKGKDYDKIWNRERDESPLKTITRS
                     QSEKLAALLGNEAIVVDWAMRYGNPATAEVIARLSALGCDRLLLVPLYPQYAAATTAS
                     ACDQAFRALMQLRWQPSVRVVPPYYDEPAYIQALAQSLRAAIARLARAPEAVVISFHG
                     MPQRLVDLGDPYYRQCLATSELVRAQLAWPQAQWHTTFQSRFGREPWLEPATIDSMER
                     LGKAGVRHLLLAAPGFAADCLETLEELDMENRAVFLANGGETFSYVPCLNDSAAGLSV
                     LEALVRRELSGWI"
     gene            4638..5624
                     /locus_tag="VFR00_14490"
     CDS             4638..5624
                     /locus_tag="VFR00_14490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEU0160527.1"
                     /translation="MGGFEAFVVVFLVLAAVALWSTVKMVPQGYNYTVENFGRYTRTL
                     NPGLHILVPVMERVGAKMNMKEQVIDIPSQDIITRDNAMVTVDGVNFYQVLNAAKAAY
                     EVDRLEGAIVNLTMTNIRTVMGSMDLDELLSNRDTINARLLQVVDAATEAWGVKVTRI
                     EIKDIAPPRDLVDSMARQMKAERDKRAQVLEAEGLRQAVVLKAEGEKQAVMLAAEGRK
                     EAAFRDAEARERAAAAEAKATEMVSQAIAGGNVQAINYFVANNYVKALEAIARSPNQK
                     VLMMPLEAASVIGSLAGLAEITREAFGRHDGGAQAAPAGRAAAPRNSSVPST"
     gene            5664..6122
                     /locus_tag="VFR00_14495"
     CDS             5664..6122
                     /locus_tag="VFR00_14495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HEU0160528.1"
                     /translation="MPSLSALLSGFGAWNWFILGALLFTLETFLPGVHFLWFGIAAVV
                     VGGLALLTGITWPWQIIAFGVLAIATVFWVRRYVRPDVARSDLPNLNERSQQYIGRSL
                     IVEQAIQNGRGKVRVGDTLWQAEGPDLPVGTWVKVTAAHGTILVVERASA"
     gene            6109..6531
                     /locus_tag="VFR00_14500"
     CDS             6109..6531
                     /locus_tag="VFR00_14500"
                     /inference="COORDINATES: protein motif:HMM:NF014062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HEU0160529.1"
                     /translation="MPRPNQDPGVVTWTLASISLALPWLGLGLSVWGLYRLSLAERAG
                     WWLLLAGLATFVADMLIDLIWAHPAVSRSDEPNLNRRAAQLVGRVLVLTDAIEGGRGR
                     VRVGDTLWQVEGPDLPRGAEVKVAAANATLLQVERAGP"
BASE COUNT         1143 a         2198 c         2073 g         1119 t
ORIGIN      
        1 tcctgatggc ggccgaaccc ctcgatgcct tctatcccat cgtccccgac gtggcctggc
       61 tccgccgcct cgtgccgctc gggataagga ccgttcagtt gcgcctgaag gacgccgagc
      121 ccgaacgggt tcgccgcgaa atcgctgaaa gccttgagat ttgtgcgcgc cataactgtc
      181 agctcattgt caacgactat tggcgcgagg ccttggagct cggcgcggat ttcattcacc
      241 tgggccagga ggacttcgcc gccgccgaca tcggcgccat tgcggcaaag tcgctacgcc
      301 tgggcatcag tacgcatagc gaggcggagt tggccattgc cctctcggcg cggccgacct
      361 acgttgccct cggccccatt ttcgagacca agctcaaggt catgcaatgg gcgccgcagg
      421 gcttgcaacg cattcgacag tggaaagacc gaattgggcc cctcccgctt gtggcgattg
      481 gcggcattac ccccgagcgc gcgggcgacg tctgggcggc cggggcggac agtgtcgccg
      541 ttattaccga tttcttcacg cattcaaatc cgacggcgcg cgtgcgcgaa tggatcgctt
      601 tggcgcgcgc tgagggtccg cggcgcgcat aaacgacggg ggtggctttt cagccatgct
      661 agtgtcctcg atcaatcgca gcatgggatg agcacgcgcg gcgcatagcc cgcccatttt
      721 tccgtatggc gtatcgtctc aagttgaaga ccacgctggc gcacgaggtg cgccgcattg
      781 cccttgagca aatcgagatc gcgcgtgggg cgcttgcccg ccccgaggat cgtgctgccg
      841 ccgtgcacga tgcgcgccga tcgctaaagc gattgcgcgc catgctgcgt ctcgtgcgcc
      901 cggccctcgg cgagacctgg ttcaagcaaa gcaaccgcca gctggccaac ctcgggcagc
      961 gcttggcgct cagccgcgat ctcgacgtca tgatcgtgac gctgagcaag ctcgagagcg
     1021 gcgccggcgg cctgcccaag ccagtggcag accgcttgcg cagcgccgtg gcccggtcgc
     1081 gcaacaaggc cgatagcgcc ggtacacccg gcgccgatcg cagtctcgct cgcgcccttc
     1141 agcgcgcgca aacgctgttt gccagcgagc ggctccgggg cgttggcctc gagcacgtcg
     1201 ccgccggcct ggagcaaagc taccgcaagg cccggcgcac gttccgcgag gcatacgcgg
     1261 cggccagcga cgaggccttc cacaattggc gcaaagccac acaagcgcac tggcgacata
     1321 tgcagctgct cggccgcagt tggccggaga gcatcggcgc gcgggccgcc gaagccaaag
     1381 agctctcgcg cctgctgggc gaggaccatg atctgtccgt gctcatcggc tttgcgcaaa
     1441 gccccggttg cggcgggctt gcgcgcgccg atctcgaaac cctgaccacc gcctgtctca
     1501 aggggcagcg gcagatcaga gacgtcgctc gctttcacgg cgagcgcctg tttgccgatc
     1561 ggccgcggca tctgaaaaag cgcatcttgc gctattggaa agcggccgag cagctctcga
     1621 ccttgttgcc gcccgacggg gaggcgacgc cgcaaaaccc cattccggcg cgcccacggc
     1681 cgccgcgagc gacccaggtc gagcgcgtgg gcgtgcgcgc cgcggccggc aaaggtgccg
     1741 cgcagcgccg cagccgtcgg ggccaagccg tggacgagaa cccgtgaacg caaacggccg
     1801 aggcaggccc gccgctggcg ggccaggctg cgtgtcacgt caggcctgtg gacgcccacg
     1861 ccgcacgcca gcaggttcag gttgcgcgcc acgtgagcgt ttgctagtcg ccaggagacg
     1921 tgagcgtgcc cgccgcacgg ggcaagacta gcggcgccgc gagagccgcg gcagtgcgct
     1981 agcccaattg acagatccga cgtttgttga ggacatcgag gcatgcgcat cgcaatgatc
     2041 ggcacgggct acgtcggctt ggtctcgggc gcatgcctcg ccgattttgg ccacatcctg
     2101 acctgcgtcg acagcgacag cagcaagatc gccgccctca agcgcggcga cattccaatc
     2161 ttcgaacccg gccttgcaga cctcgtcgcc cgtaatgtgc gcgaaggccg gctagccttc
     2221 agcaccgagc ttgcgcccgc cgtcaaaacg gccgaggcga tttttatcgc cgtcggcacg
     2281 ccgtcgcgac gcggcgacgg gcatgcggac ctcagctacg tcttccaagc ggcgcgcgag
     2341 atcgccaccg cgctcgacga ccacaaggtg attgccacca aatcgacggt gccggtcggg
     2401 accggcgacg aggtggcgcg catcattgcc gaggcgcggc caaacctgac gttttccgtg
     2461 gtgtccaacc ccgagttttt acgcgagggc gccgccatcg ccgatttcaa acgacccgat
     2521 cgcatcgtca tcggggccga agatgagcgc ggacgcaagt tgatgcggga cgtctaccgc
     2581 cccctttacc tcaaccaggc tcccatgctg ttcacggacc ggcgcaccgc ggagctcatc
     2641 aagtacgcgg ccaatgcgtt cctggcgacg aaaatcacct tcatcaacga aatcgcagat
     2701 ttgtgcgagc gcgccggcgc caatgtgcag gacgtggcgc gcggcatcgg tctcgacaac
     2761 cgcatcggcg ccaagttcct caacgccgga ccgggtttcg ggggctcctg ctttcccaag
     2821 gacaccttgg ctttgatgaa gacgggccac gacttcggcg tgccaagccg catcgtcgaa
     2881 accgtgatcg ccgtcaacga ccagcgcaag cgcgccatgg ggcgcaaggt gatcgacgtc
     2941 tgcggtggca acgtgcgcgg ccgcaccatc gccgtgctgg gactgacctt caagcccaac
     3001 accgatgata tgcgcgatgc cccctcgatt gcggtgatca cggcgctgca ggacgccggg
     3061 gcgaacatcc gcgcctacga tccggccggc atgaagcagg caaagagcgt gctcgacaac
     3121 gtcgaataca gcagtgaccc ctatgcctgc gccgagggcg ccgatgccct cgtcattctc
     3181 accgagtggg acgagtttcg cgcgctcgat gtcgatcgcg tgcgcgccaa gttgaaaagg
     3241 cctattttgg tcgatctgcg caatatctat cggccggaag agatggcacg ccaggatttc
     3301 acctacgtca gcgtcggccg tccgaccgcg cgcaaagagg catgaggatc ggccagcgcg
     3361 gcggtgccag aacggcaacc gagcgccaac gagcgaggcc caggtgaacg cgcaagatac
     3421 gccaagctcg gttgcggtac ccgagcccaa atcgagcgcc gggcgcggcc gtatcggcgt
     3481 gctcctcctc aatctcggca cgccggacgc cacgagctac tggccgatgc ggcgctattt
     3541 gcgggaattt ctttccgatc ggcgcgtcat tgacgtgccg cgcctgaagt ggtggcccat
     3601 tctcaatctc gtcattttga ccgtcagacc cggccctaaa ggcaaggatt atgacaagat
     3661 ctggaaccgg gaacgcgacg agagcccgct caagaccatt acccgatcgc aaagcgaaaa
     3721 gctggcggcg ctgctgggca acgaggccat cgtcgtcgat tgggccatgc gctacggcaa
     3781 tccggcaacc gccgaagtca tcgcgcggtt gagcgcgctc ggttgcgacc ggctgctttt
     3841 ggtgccgctc tacccacaat atgccgcggc gacgacggcg agtgcgtgcg atcaggcctt
     3901 ccgtgccctg atgcagctgc gctggcagcc cagcgtgcgc gtggtgccgc cctattatga
     3961 cgagcccgcc tacattcaag ccttggcgca atcgctgcgc gcggccattg cccggctcgc
     4021 gcgcgcgccc gaggctgtgg tgatctcctt tcacggcatg ccgcaacgcc tcgtggatct
     4081 gggtgatcct tactatcgac agtgcctcgc gacctccgag cttgtgcgcg ctcaattggc
     4141 gtggccgcag gcgcagtggc acacgacctt ccaatcccgc ttcggccgcg agccgtggct
     4201 cgagcccgcc accatcgaca gcatggagcg gctcggcaag gccggcgtgc gccatctcct
     4261 cctcgccgct cccggctttg ccgccgactg cctggaaacg ctggaggaac tcgacatgga
     4321 gaaccgtgcc gtgttcctcg ccaacggcgg ggagaccttc agctacgtac cctgcctcaa
     4381 cgatagcgcg gcgggcctgt ccgtgctcga ggcgttagtg cgccgcgaat tgagcggctg
     4441 gatttgaggg cggcgccgag cctgaagtgg gcaagcgcgc acgcccggcg cttttttgaa
     4501 aagcgattga aaacactttc gaattcatta tccgtaaatc tgtcatttac ctgtcatttg
     4561 cattcccact ctaaaacttg ccgcagttcg tacctaaata acaaacacgt tccgaggcag
     4621 gtcacacgcc catgaatatc ggcggtttcg aagcttttgt cgtggttttc ctggtgctgg
     4681 ccgccgtcgc cctatggtcg acggtcaaga tggtgccgca gggttacaac tacacggttg
     4741 agaactttgg gcgctacacg cgcacgctca atccgggctt acatattctg gtgcccgtca
     4801 tggagcgcgt cggcgcgaaa atgaatatga aggagcaggt gatcgatatt ccctcgcagg
     4861 acatcatcac ccgcgacaac gccatggtca ccgtcgatgg cgtcaatttc tatcaggtcc
     4921 tcaacgccgc caaggccgcc tacgaggtcg accgcttgga gggcgccatc gtcaacctca
     4981 ccatgaccaa tattcgcacc gtcatgggat cgatggatct cgacgagttg ctctccaatc
     5041 gcgacaccat caacgcgagg ctactgcagg tggtggacgc ggccaccgaa gcgtggggcg
     5101 tcaaggtcac ccgcatcgaa atcaaggata tcgcgccgcc ccgcgacctc gtcgacagca
     5161 tggcgcgcca gatgaaggcc gagcgcgaca agcgcgcgca agtcctggag gccgagggcc
     5221 tgcgccaggc cgtcgtgctg aaggcggaag gcgaaaagca ggcggtgatg ctggccgccg
     5281 aaggccgcaa ggaggccgcc ttccgcgatg ccgaagcgcg cgagcgtgcg gctgcggccg
     5341 aagccaaggc caccgaaatg gtgagccagg ccatcgccgg cggcaatgtg caggccatca
     5401 actacttcgt cgccaacaac tacgtcaaag cgctggaagc catcgcccgc tcgcccaacc
     5461 agaaagtgtt gatgatgccg ctcgaggccg cctcggtgat cggctcgctg gcagggctcg
     5521 ccgaaatcac gcgcgaggcc ttcggccgac acgatggagg tgcgcaggcc gccccggcgg
     5581 gccgcgccgc cgcgccccgc aatagcagcg tgccctcaac gtaacgcccc ttcgatcccg
     5641 tccgcccatc aggatcccgt gccatgccgt ccctgtccgc cctgctctct ggcttcggcg
     5701 cctggaactg gttcattctc ggcgcgctgc tgttcacgct cgagactttc ctgcctgggg
     5761 tgcacttcct atggttcggc atcgccgccg tcgtggtcgg cggcttggcg ctgctgaccg
     5821 gcatcacctg gccctggcag atcatcgcct tcggtgttct cgccatcgcc acggtgtttt
     5881 gggtgcgccg gtacgtgcgt cccgacgtgg cccgcagcga tctgcccaac ctcaacgagc
     5941 gcagccagca gtacatcggc cgctcgctga tcgtcgagca ggcgatccag aacggccgcg
     6001 gcaaggtgcg tgtcggtgat accctgtggc aagcggaggg tccggatctg cccgtcggca
     6061 cctgggtcaa ggttaccgcc gcccacggca ccatcctggt cgtcgagcgt gcctcggcct
     6121 aatcaggatc ctggcgtcgt cacttggacg ctcgccagca tcagcctggc cttgccgtgg
     6181 ctcggcctcg gcctcagcgt gtggggcctc tatcgtctat cgctggccga gcgggcgggc
     6241 tggtggctgt tgctggcggg gcttgccacc ttcgtcgccg atatgctcat cgatttgatc
     6301 tgggcgcacc cggccgtcag ccgcagcgac gagcccaatc tcaatcggcg cgctgcccaa
     6361 ttggtcggtc gtgtcctcgt cctcaccgac gccatcgaag gcggacgcgg gcgcgttcgg
     6421 gtcggcgata cgctctggca agtcgagggc cccgatcttc cccgcggcgc cgaagtcaag
     6481 gtcgctgccg ccaacgccac cctgctgcag gtcgaacgcg cgggcccctg agt
//
LOCUS       DASTMT010000626         3282 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_4853282, whole genome shotgun sequence.
ACCESSION   DASTMT010000626 DASTMT010000000
VERSION     DASTMT010000626.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3282
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4853282"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(100..630)
                     /locus_tag="VFR00_14505"
     CDS             complement(100..630)
                     /locus_tag="VFR00_14505"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEU0160530.1"
                     /translation="MPDLKSLIRTIPDYPKKGVMFRDVTTLLGDPQGFKAAIEQMAAP
                     FAAAGVQGVAGIEARGFILGGAIADRLGCGFVPFRKKGKLPWKTIGQEYTLEYGVDII
                     EMHEDAIDPGEKILIVDDLIATGGTAEAAAKLVRRSGGEIVGATFIIDLPELGGAKRL
                     AGLGIKFHALMAFAGH"
     gene            778..1188
                     /locus_tag="VFR00_14510"
     CDS             778..1188
                     /locus_tag="VFR00_14510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008034007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU0160531.1"
                     /translation="MKIKLHHVNFCSSNVPALDAFYRDVLDLQPEPSLEGGRIRDRDG
                     YPGQVAFRTDGTTQFHLAEKDLGITFRTGQAINPVERGHIAFRTDDIEAFKKRLKEKG
                     IPFSDYGAWAMRGWEQIFFYDPDGNIIEVHQVKE"
     gene            complement(1273..2865)
                     /locus_tag="VFR00_14515"
     CDS             complement(1273..2865)
                     /locus_tag="VFR00_14515"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HEU0160532.1"
                     /translation="MQEYDYIVVGAGSAGAALAARLSEDGSNRVLLVEAGRATHPYAR
                     FPISFGLLINNPSANWLYASEPEASTANRAIPVPRGKLLGGSSAINGLVWVRGQPLDY
                     DTWAQMGARGWSWQDVAPLFRRIENYEKGAANGRGVGGPLRVSEVGDENPLYDALFKA
                     AVAAGYKLNPDYNGEDQEGMVKTQASIWRGQRMSVAHCYLKPAMKRANLHVLAEAPTR
                     RVCLEGRRCVGISYLRHGKLVEARARREVILCAGAIATPQLLELSGIGRPEILKAHGI
                     EVKHALPAVGENFRDHLNARIVWRVNDRRVSYNHKARGVRAANEVLRYVTTRGGFFSL
                     PSAPLLAFLKTRPELATPDVQMHLVPYAIKDPKQRKLQDFPSMTVSVYQLRPESLGSI
                     HIRSADPDQQPAIRFNFLADPIDQRTMVDGFRMIRRIVGTAAMDALRGEEFSPGRDVE
                     TDAEILTWIRNNAQTAYHPIGTCRMGRAPNAVVDERLKVIGLEGLRIADASIFPTMPS
                     GNTNAPAIMVGEKAADLIRSAA"
     gene            complement(3027..>3282)
                     /locus_tag="VFR00_14520"
     CDS             complement(3027..>3282)
                     /locus_tag="VFR00_14520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016842927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEU0160533.1"
                     /translation="GAANRDPAEFPNPDRFDVAREPNRHLAFGSGAHVCAGLNIARLE
                     GRIGIGRLLARFPQYRLSGPPIRGGRARFRGFLSLPAEVA"
BASE COUNT          568 a         1060 c         1067 g          587 t
ORIGIN      
        1 gcggccgggc attctcgccc cggggacgtc tcgccccggg gatatggggg gccgcggcgc
       61 cgcgccggcg acagccaccg gatcgcgcta ggccaatcgc taatggccgg cgaaggccat
      121 gagggcatga aacttgatgc cgaggccagc cagccgcttg gcgccgccga gctcgggcaa
      181 atcgatgatg aaggtcgccc cgacaatctc gccgcccgaa cgccgcacga gcttggccgc
      241 cgcctcggcg gttccacccg tggcgatcag gtcgtccacg atcaggatct tttcaccggg
      301 atcgatggca tcctcgtgca tttcaataat gtcgacgccg tactccaagg tgtattcctg
      361 gccaatggtc ttccagggca gcttgccctt tttgcggaat ggcacgaaac cgcagccgag
      421 acggtcggcg atggctccgc ccaatataaa gccgcgcgcc tcgatgccgg ccacgccttg
      481 cacgccggca gccgcgaagg gtgccgccat ctgttcgatg gcggccttga agccttgcgg
      541 atcgcccaac aaggtggtga cgtcgcggaa catcaccccc ttcttgggat agtccgggat
      601 ggtgcggatc aggcttttga ggtcgggcac ggctcggggc tccgttcaga gagggcagtc
      661 atagcaagcc ccgattggca ggaaaacacc agcacaaccc cgggcgtaat tgcgactttc
      721 gaggcagaca gggcgcgctt aaactggcgc ccgaccagac ggagccgagg accccccatg
      781 aagatcaagc tgcaccatgt caacttctgc tcgagcaatg tgccggcgct cgacgccttc
      841 taccgggacg tactcgatct tcaacccgaa ccttcactag aagggggacg cattcgcgat
      901 cgggatggct acccgggcca ggttgccttc cgcacggacg gcaccacgca gtttcacctg
      961 gccgagaagg atctgggcat caccttccgc accggccagg ccatcaaccc ggtcgagcgt
     1021 ggtcatatcg ccttccgcac ggacgatatc gaggccttta agaagcgctt gaaagagaag
     1081 ggcattcctt tttccgatta tggcgcctgg gccatgcggg gatgggagca gatctttttt
     1141 tacgaccccg acggcaacat catcgaagtg caccaggtca aggaatgacg ccgcagggcc
     1201 gcgtgcggtc gcagcggtgc tcggcagggc tattgtgggc cgcactacga ggcgggggtc
     1261 gcttggcctc gctcacgccg cgcttctgat caggtctgcc gccttttcgc cgaccatgat
     1321 tgccggggca ttggtgttgc ccgatggcat ggtcggaaag atcgaggcgt cggcgatgcg
     1381 caggccctcg aggccgataa ccttgagccg ttcgtccacc acggcgttgg gggcgcggcc
     1441 catccggcag gtgccgatcg ggtgataggc ggtctgagcg ttgttgcgga tccaggtcaa
     1501 gatctccgcg tccgtctcca cgtcccggcc cggcgaaaac tcctccccgc gcagcgcatc
     1561 catcgccgcg gtcccgacga tgcgccggat catgcggaag ccgtccacca tggtgcgctg
     1621 gtcgatggga tcggccaaaa aattgaagcg gatcgccggc tgctggtccg gatcggccga
     1681 gcggatatga atggagccga ggctctccgg gcggagctga tagacggaaa ccgtcatgga
     1741 cgggaagtcc tgcagcttgc gctgctttgg gtccttgatg gcatagggta ccaggtgcat
     1801 ttgcacgtcc ggcgtggcga gttcgggacg tgtcttcaga aatgccaaca ggggcgccga
     1861 gggcaggctg aaaaagcccc cgcgcgtcgt cacatagcgc aagacctcat tggcggcgcg
     1921 cacaccgcga gccttgtgat tgtaggagac gcggcggtcg ttcacgcgcc agacgatgcg
     1981 ggcgttgagg tggtcacgaa agttttcgcc gactgccggc aacgcatgct tcacctcgat
     2041 gccatgggct ttgagaatct caggcctgcc gatgccggaa agttccaaaa gctgcggcgt
     2101 ggcgattgcg ccggcgcaca ggatcacctc gcggcgggcg cgcgcctcca ccagcttgcc
     2161 atgtcgcaga tagctgatgc cgacgcagcg cctgccctcg aggcacacgc gccgggtcgg
     2221 ggcttcggct aggacgtgca ggtttgcccg tttcatggcg ggcttgaggt agcagtgggc
     2281 cacgctcatg cgctgtccgc gccagatact ggcctgggtc ttgaccatcc cctcctggtc
     2341 ttcgccgttg tagtcgggat tgagcttgta gcctgcggcg actgcggctt tgaacagagc
     2401 atcgtagagc gggttctcat ctcccacctc cgatacgcgc agcggaccgc cgacaccgcg
     2461 cccattggcg gcgccttttt cgtagttctc gatgcgacga aataggggcg ctacatcctg
     2521 ccagctccag ccgcgcgcgc ccatctgcgc ccaggtgtca taatcgaggg gttggccgcg
     2581 aacccagacc agtccattga tcgcgctgga gccgccgagc agtttgccgc gcggcacggg
     2641 aattgcccgg tttgccgtgc tcgcctcggg ttcggaggca taaagccagt ttgcgcttgg
     2701 attgttgatc agcagcccga aactgatggg aaagcgcgca taggggtggg tggcccgtcc
     2761 ggcttcgacc agcagcacgc ggttgcttcc atcttcactc aagcgtgccg ccagggcggc
     2821 gccggcggaa cccgccccga caacgatgta gtcgtactct tgcatgtctg ttcttcccca
     2881 cggcccttcg gcggttctgc caccgcctct tcctcagcct agcctcttgc ctcctatgcc
     2941 cgggggtcgc ggcccaggtc aaatttgcca aagctcagtt gctgtcgcag ccatgccatg
     3001 ccgcccgccc cagggctgtc gccgcctcag gcaacctcgg cgggcaggga gagaaagcct
     3061 cgaaaacggg cgcgcccgcc ccggatcggc ggcccgctca agcgatactg cgggaagcgc
     3121 gccagcagcc ggccgatccc aatgcgcccc tcgaggcgcg cgatgttgag cccggcgcag
     3181 acgtgggcgc ccgagccaaa cgccagatgc cgatttggct cccgcgccac gtcgaagcga
     3241 tcgggatttg ggaactcggc cgggtcgcga ttggccgcgc cg
//
LOCUS       DASTMT010000627         5871 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_5180411, whole genome shotgun sequence.
ACCESSION   DASTMT010000627 DASTMT010000000
VERSION     DASTMT010000627.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 5871)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5871)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5871
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5180411"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(127..612)
                     /locus_tag="VFR00_14525"
     CDS             complement(127..612)
                     /locus_tag="VFR00_14525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEU0160534.1"
                     /translation="MPTPQELEAKFWKALRSDMTMMIGLVGVDDGHVRPMTAQLEEDH
                     GPIWFFTAKDNAIVQQLARSDRAIATFASKGHELFATVHGRLSVNNDHRVIGRLWNRF
                     VAAWYEGGMDDPKLALLRLDAESAEIWLDASSLIAGIKMLLGADPKTDYRDKVADVAL
                     R"
     gene            complement(884..1789)
                     /locus_tag="VFR00_14530"
     CDS             complement(884..1789)
                     /locus_tag="VFR00_14530"
                     /inference="COORDINATES: protein motif:HMM:NF022834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HEU0160535.1"
                     /translation="MLQRAEALGFGPRCKATLVGAIAARALCDGAHGHVRACFEHSLY
                     LQLGSGVLCLGAENLGAGPLNLLCTPMPEGSVRPRRNPGDAVRIEGETLVVGELMIEL
                     GDADPWWPAPVGPWDRSSLVRGLAAGRAALGVLQPQDGLALLAPDARSQQGSMGRVAA
                     APIDHLGDLLRAEPTVEPDAARIAPLIGLGPGLTPSGDDVLGGFLVALALVGRIALRD
                     RLWRAVHSAIAERTTEISCAHLAAAAEGQGGAPLHSALSAIMRGASAGIEGACVALGR
                     VGHTSGWDALAGGLLVLEATAKVTT"
     gene            complement(1798..3282)
                     /gene="fdrA"
                     /locus_tag="VFR00_14535"
     CDS             complement(1798..3282)
                     /gene="fdrA"
                     /locus_tag="VFR00_14535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018273459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HEU0160536.1"
                     /translation="MSRSVRNAVRRAFYLDSVALMRLSREIAALEGVLDAALMMATPA
                     NKAILAEAGLIDAEAEAAGANDLILAVSAEAPDRADAALAAAQVLLAPRARPNAAPRG
                     HSARSLRTALGAQPDANLALISVPGDYAAGEARRALGCGLDVMIFSDNVPLAEEVALK
                     RQAQALGRLLLGPDCGTAILGGAPLGFANRVPRGDIGLIGASGTGLQEVSVLIAAAGR
                     GVSEAIGVGSRDLSLEVEALATLAAIDLLDQDPATARIVLISKPPHPEVVAKVLARIG
                     RSPKRFTLCFLGAAPLELPGNATAVPTLRAAAEDVLGSSRTGKPDVAPTAKPRAGHIL
                     GLYAGGTLGAEAQLLLIAAGRIVASNTAVPGARALSGAPAGVDQIIDLGADEFTRGRP
                     HPMIDPASRCERLGRALEERGVAAVLLDVVLGYGAHADPAGEIATVIERAPANRPHVV
                     ACVVGTEADPQVRSRQVAALRAAGVLVARSNAGAAELALALAQG"
     gene            complement(3279..4781)
                     /locus_tag="VFR00_14540"
     CDS             complement(3279..4781)
                     /locus_tag="VFR00_14540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019196112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HEU0160537.1"
                     /translation="MSRSCGDGEAGFANGIIEKEGLFLRMARPAPPRLLTSPISVVNI
                     GLPQFAADLRSCGVPVTHVEWSPPALSAPGLAHVVARLSEDPAIAAANQEALARLLAA
                     EPVLIDVVPAVEAISALAEGRILLHAGPPVAWERMCGPMQGAICGAIVFEGWAPDLAA
                     AAELVAAGGVALAPNHHFDAVGPMTGITTRSMPVLVVENRRFANRAYATLNEGLGKVM
                     RFGGNDWEVLKRLAWLRDVLGPALGRALRAGNGVALKPLIARGLGMGDEMHQRNVACT
                     GLILREIAPALARTFEATGLAEVLAFIAGNDQFFLNIAMALGKAMTDPVRNIAHSSIV
                     TAMCRNGTDFGIRVSGTGDTWFTAPVEMPVGLYFPGFSAADANPDMGDSAIVETIGLG
                     GFAMAAAPAVVGFVGAGSAAEAATFTQAMREITSAENPHWTVPALDFAGVPTGIDVRL
                     VVETGIVPTINTGIAHRQAGVGQVGAGVVRAPMACFTAALAALGAAREAA"
     gene            complement(4778..5206)
                     /locus_tag="VFR00_14545"
     CDS             complement(4778..5206)
                     /locus_tag="VFR00_14545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018459291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEU0160538.1"
                     /translation="MSATATETRHDLETASAAQIVEKFLIASMVPDPDTAARYISPDL
                     KITFTGGRSYRHPSETAAFNARRYKWVKKKMERTDVVAGARETIVYNLGTLYGEWPDG
                     TPFEGNRYVDRFVVRDAKIVAMDVWNDSAERLLVLNGIEA"
     gene            5444..>5871
                     /locus_tag="VFR00_14550"
     CDS             5444..>5871
                     /locus_tag="VFR00_14550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015857664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0160539.1"
                     /translation="MHADVALPRIARRFLIAGALAFAAALPAAAQETVKLGLVAAMSG
                     QSAKSGEAIVRGLSLAIDEINARGGLLGKKAELIVRDDESNPAKGAVAARELVQREKV
                     AALFGGLDTPVSLAIVPFANQNKVPFMGVWAAGTPITRNGA"
BASE COUNT          980 a         2044 c         1958 g          889 t
ORIGIN      
        1 ccgccatctg ggcgatggac gcgatgatcg tcgtgaccgc gctttacctg ctgttcttcg
       61 ccggcagcgc cgcgaccgtt cccgcctgac cgccggcgcg gccagggcgc gccgtttgcg
      121 gtacggctac cggagcgcga cgtcggcgac cttgtcgcgg tagtccgtct tggggtcggc
      181 gccgagcagc atcttgatcc ccgcaatgag gctggaggcg tccagccaaa tttccgcgct
      241 ctccgcgtcg agccgcagca acgcgagctt gggatcgtcc atgccgcctt cgtaccaagc
      301 ggcaacaaat cgattccaca gccggccgat cactcggtgg tcgttgttga cggacagccg
      361 cccgtgcacc gtcgcaaaga gttcgtgacc cttggaggca aaggtagcga tcgcgcggtc
      421 gctccgagcc agctgctgca cgatcgcatt gtcctttgcg gtgaagaacc agatcggtcc
      481 gtggtcttcc tccagctggg ccgtcatggg acggacatgg ccgtcgtcga cgcccaccaa
      541 tccgatcatc attgtcatgt cggacctgag agccttccag aatttggcct cgagttcttg
      601 aggggtcggc atcgccttct ccatcgttgc tgtttttgaa acttgttctg gtgacacgaa
      661 gttccgcccc gtcagagcgg gatgcggtgc ccttaagccg cgcgagagtg ccgattgccg
      721 cgatgtcgag tgtgtccccg ccgctgccaa gcaagcgggc tgcgcccgct gccggacgca
      781 aggtacgggg gcttaccagc gcacccgcat gcggcgccga tcgtcgcagc gaccctcacc
      841 gggccgcttg caagctgcag ctgacccggc attcacttag cttttaggtt gtgaccttgg
      901 cggtcgcctc cagcaccagg aggccgccgg ccagcgcatc ccagcccgac gtgtgcccga
      961 cgcgcccgag cgccacgcag gccccttcga tcccggcgct cgcccctcgc ataatggcgc
     1021 tcagtgcgct gtgcagcggg gcgcccccct ggccttcggc tgcggccgca agatgggcgc
     1081 agctaatctc cgtcgtgcgc tcggcgatgg cgctgtgcac ggcccgccac aggcggtccc
     1141 tgagcgcaat acgccccacc agcgccaatg ccaccaagaa gccgcccaga acgtcatcac
     1201 ccgacggcgt aaggccgggg ccgagcccga tcagcggtgc gatccgggcg gcatcgggct
     1261 caaccgtagg ctcggcccgc aagagatcgc ccaaatggtc gatgggcgcc gcggcaacgc
     1321 ggcccatcga cccctgctgc gaccgagcgt cgggggcgag aagcgcaaga ccgtcctgcg
     1381 gctgcaatac gcccagcgcc gccctgccgg cagcaagccc gcgcacaagg ctcgatcgat
     1441 cccagggacc aacgggcgcg ggccaccagg ggtccgcgtc gccgagctcg atcatgagct
     1501 cgccaacgac caaagtctcg ccctcgatgc ggacggcatc tccggggttg cggcgcggcc
     1561 tgacgctgcc ctcgggcatt ggcgtgcaaa gcaggttgag cgggccagcg cctaggttct
     1621 ccgccccaag acataggacg ccggagccga gctgcaggta tagcgaatgc tcgaagcaag
     1681 ccctgacatg gccatgcgcg ccgtcacaca acgcgcgcgc ggcgatggcg ccgaccaagg
     1741 tcgccttgca gcgcgggcca aagcccagag cctccgcgcg ttgcaacatg ctgatcctca
     1801 gccctgcgcc agggccagcg ccaactcggc ggcgccagca ttggatcgcg cgaccagcac
     1861 gcctgccgcg cgcagcgcgg cgacctggcg cgagcgcacc tgagggtcgg cctccgtgcc
     1921 aacgacgcaa gccaccacgt gcggtcgatt ggccggcgcg cgctcgatga cggtggcaat
     1981 ctcgccggcc ggatcggcat gggcgccata gcccagcacc acatcaagaa gcacggccgc
     2041 gacgccgcgc tcctcgaggg cgcggccgag ccgctcgcag cggctcgccg gatcgatcat
     2101 gggatgtggg cgaccgcgcg tgaactcgtc ggcgccaagg tcgatgatct gatccacgcc
     2161 tgccggggcg ccgctgagcg cgcgcgcgcc aggcacggcc gtattggagg ccacaatgcg
     2221 gcccgcagcg atcaagagca gctgcgcctc ggcaccgagc gtgccgccgg cataaagacc
     2281 gaggatgtga ccggcccgcg gctttgcggt tggcgcaacg tcaggcttcc ccgtgcggct
     2341 ggaccccagc acatcctcgg ccgccgcgcg cagcgtcggg acggcagtgg cattgccagg
     2401 cagctctagc ggcgcggcgc ccaaaaagca gagcgtgaaa cgcttggggc tgcggccgat
     2461 gcgggccaac acctttgcca ccacttcggg gtgcggcggc ttggaaatca gcacgatgcg
     2521 cgccgtcgcc ggatcctgat cgaggagatc gatggccgcc agggtggcga gcgcctcgac
     2581 ctcaaggctc aaatcgcggc tgccgacgcc gatcgcctcg gaaaccccgc ggcccgccgc
     2641 ggcgatcagc acgctcacct cctgcagccc ggtgccggag gcaccgatca ggccgatgtc
     2701 cccgcgcggc acccgattgg cgaaccccag cggggcaccg ccgaggatgg cggtgccgca
     2761 gtccgggccc agcagcaggc gacccagcgc ttgcgcctga cgcttcaggg cgacttcctc
     2821 ggcgagtggc acgttgtcgc tgaaaatcat gacgtccagg ccgcaaccga gcgccctgcg
     2881 cgcctcgccc gcggcgtagt caccgggcac cgagatcaag gccagattgg cgtctggctg
     2941 cgcgccgagc gcggtgcgca agctgcgcgc agaatgcccg cgcggcgccg cgttgggtct
     3001 tgcccgtggc gccagcaaaa cctgcgcggc ggcgagcgcc gcatccgcgc gatcgggtgc
     3061 ctcggcgctc accgccaaga tcaaatcgtt ggcgccagcc gcctcggcct cggcgtcgat
     3121 gaggccggcc tcggcgagaa tggccttgtt ggccggcgtc gccatcatga gcgccgcatc
     3181 cagcacgccc tcgagcgccg ctatttcgcg cgacaggcgc atcaaggcga ccgagtccag
     3241 gtagaaggcg cgccgaacgg cgttgcgcac gctccggctc atgctgcctc ccgtgcggcg
     3301 ccaagcgccg ccagcgccgc ggtaaagcat gccattggcg cacgcacgac gccggcgccg
     3361 acttggccga ccccggcttg gcgatgggcg atccccgtat tgatggtcgg aacaatgccg
     3421 gtctcgacga ccaggcgaac atcgatgccc gtcggcacgc cggcgaaatc gagggcgggc
     3481 acggtccagt ggggattttc ggcgctggtg atctcgcgca tcgcctgcgt gaaggtggcc
     3541 gcctcggccg ccgagccggc accgacgaac cccaccacgg ccggggccgc cgccatggca
     3601 aagccgccga gaccgatggt ctcgacgatg gccgagtctc ccatgtccgg attggcgtcc
     3661 gccgccgaaa agccggggaa gtagagcccg accggcattt ccaccggcgc cgtgaaccag
     3721 gtgtcgcccg tgccgctgac gcgaatgccg aagtcggtgc cgttgcggca catggccgta
     3781 acaatggaag agtgggcgat gttgcgcacc ggatcggtca tcgccttgcc gagcgccatg
     3841 gcgatgttga gaaagaattg gtcattgccg gcaatgaagg ccaaaacctc ggcgagaccg
     3901 gtggcctcga acgtgcgcgc cagcgctggc gcaatctcgc gcaaaatcag ccccgtgcag
     3961 gcgacattgc gctggtgcat ctcatcgccc atgccgagcc cgcgcgcgat caagggcttg
     4021 agcgcgacgc cgttgccagc gcgcaaggcc ctgcccagcg ccggtcccaa tacatcgcgc
     4081 agccaggcca ggcgcttcag cacctcccag tcgttgcccc caaagcgcat gaccttgccg
     4141 agcccctcgt tcaaggtggc ataggcgcga ttggcaaagc ggcggttctc caccaccaat
     4201 accggcatgg accgggtggt gataccggtc atcggcccga ccgcatcgaa gtgatgattg
     4261 ggcgcgagcg caacgccgcc tgccgccacc agctccgccg ccgccgccag atcgggtgcc
     4321 cagccctcga acacgatggc gccacagatg gcgccctgca tgggaccgca catgcgctcc
     4381 caggccacgg gcggaccggc gtgcaggagg atgcggccct cagccagcgc cgaaatggcc
     4441 tcgaccgccg gcaccacgtc gattagcacc ggctcggcgg ccaataagcg cgccagggct
     4501 tcctggtttg ccgccgcgat cgctggatcc tcgctgagcc tggccacgac gtgcgcgagc
     4561 cccggcgcac tgagcgccgg cggcgaccac tcgacgtggg tcacgggcac accgcaactc
     4621 ctgaggtcgg cggcgaattg gggcaggccg atgttgacga cgctgatggg cgacgtcagc
     4681 aggcggggcg gtgcgggccg agccatgcgc aggaacaaac cctctttctc gataatgccg
     4741 ttggcgaagc cagcctcgcc gtccccgcag ctccggctca cgcctcaatg ccgttcagca
     4801 caagcaagcg ctcggcgcta tcgttccaga catccatggc gacgatcttg gcgtcgcgca
     4861 ccacgaaacg atcgacatag cgattgccct cgaacggcgt gccatcgggc cattcgccgt
     4921 agagcgtgcc gagattatag acgatggttt cgcgcgcgcc ggcaacgacg tcggtgcgct
     4981 ccatcttctt tttcacccat ttgtagcgac gcgcgttgaa ggcggcggtc tcgctcgggt
     5041 gccggtagct gcggccgccg gtaaaggtga tcttcaggtc gggcgagata tagcgcgccg
     5101 cggtatcggg atcgggcacc atcgaggcga tgaggaactt ctcgacaatt tgggcggcgc
     5161 tcgccgtctc aaggtcgtgc ctggtctcgg tggcggtagc ggacatggat ccttcccaag
     5221 ggctggcaaa cgtcgggggc gccgcaccat ggaccggcgc gcgcccgcaa tctactgctt
     5281 tataccatgc agctttgccg caaaatggtg cgacctgccc gcccaggtgc acccaagttg
     5341 ggcaaaactt gcctaatcaa ccgtaggcag cggccggcgc ctatggcata tccatgacgt
     5401 cccagggggg atcagctttc ccatgttccg aaaggtcgtt gccatgcatg ccgacgttgc
     5461 gctgcctcga attgcccggc gtttcctgat cgcaggcgcg ctcgcgtttg ccgccgcgct
     5521 tcccgccgcc gcgcaagaga cggtgaaatt gggtttggtg gcggccatgt cgggccagtc
     5581 ggcaaagtcg ggtgaggcga tcgtgcgcgg gttgtcgctg gccatcgacg aaatcaatgc
     5641 ccgcggcggg ctgctgggca aaaaggccga gctcatcgtg cgcgatgacg aaagcaatcc
     5701 cgccaagggt gccgtcgccg cccgcgagct ggtgcagcgc gaaaaggttg cagccctgtt
     5761 cggcggtctc gatacgcccg tgtcgctggc gatcgtgccg ttcgccaacc aaaataaggt
     5821 gcctttcatg ggtgtgtggg cggcgggcac gccgatcacg cgcaacggcg c
//
LOCUS       DASTMT010000628         3792 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_5786046, whole genome shotgun sequence.
ACCESSION   DASTMT010000628 DASTMT010000000
VERSION     DASTMT010000628.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3792)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3792)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3792
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5786046"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(121..825)
                     /locus_tag="VFR00_14555"
     CDS             complement(121..825)
                     /locus_tag="VFR00_14555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011089217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0160540.1"
                     /translation="MLELSDINAGYAGFQALFGVSMQVAAGEAVAVIGANGAGKTTLL
                     RVISGLLPPTSGRMTLEGRDLLAMRAHSIVAAGIAHVPESRRLFPLLSVEENLRMGAF
                     IASARARFAERREYVYGLFPRLAERKGQLAGTLSGGEQQMCAIARALMSGPKLVLLDE
                     PSMGLAPVVVSQVFDLVRRIRGEGYTVLIVEQNVRQVLKVVDRAYLLEVGRVKAEGAA
                     ANLAASDEIRAAYMGL"
     gene            complement(830..1561)
                     /locus_tag="VFR00_14560"
     CDS             complement(830..1561)
                     /locus_tag="VFR00_14560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011089218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0160541.1"
                     /translation="MAFLKVSGLTKTFGGFTAVSNVSFDVDQGEILGLIGPNGSGKST
                     TFNCIAGMYAPSKGSILLEEQEIAGLSATHVCHKGIGRTYQIPRPFRNLTLIENVALS
                     AWFGQEGKVDRAACWREAGEALALVGLPTDARTTTDGLGAAALKKLELARALATRPKL
                     LLADESLNGLDHAEMEQAALMLRRIRDEMRITIIWVEHIMGVLMRMVDRVVVLDHGEK
                     IFDGAPKAAQSNRRVIEVYLGPDQD"
     gene            complement(1632..2924)
                     /locus_tag="VFR00_14565"
     CDS             complement(1632..2924)
                     /locus_tag="VFR00_14565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014493084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU0160542.1"
                     /translation="MRLALPVVIAGGCLAAVLTATGAAAQDIKIGTIYDLTGPFAGGG
                     SKPAFIGNKIAIDMINEKGGVEGHKIVAIDADAQSKVDVAINEAERLLNEAKVDLLMG
                     VYSSAQCVPLAAKVDAAKKFLWANVCVASAVFKDKNLTYAFRPTIHSDQYGDATCSFI
                     AENAKGKLGKEPKDVKVAIIHEDGPYGVGVGVASEERCKALGIQVVHKEGYSATATDL
                     SALVTKLRRAQPDVIVHAGYNPDITLFLRQAKEAGLKFSALIGEGAGYGQIDKLRETF
                     KDDVNYIYNVDPVAGQLLDPKTLQPGVGEITAELVKRYKAETGASEVPPHASMGFNQA
                     WIFLTDVLPRAIKKYGGWDAEALRKAALDTDIPLGGTIQGYGVKFFPPGTKMAGQNER
                     STPVVMQYVDGHTKIAWPTAIRTVDPVLPLPKGHAYAR"
     gene            complement(3037..3789)
                     /locus_tag="VFR00_14570"
     CDS             complement(3037..3789)
                     /locus_tag="VFR00_14570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002710912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0160543.1"
                     /translation="MSELFAPGGYRFIPAVFQYSGGVAAAPGFRIERVRFRHPLPLAR
                     GFERAAEIITARGRPLTAFCACELRSPAPFSDATFRAFNELYAGTLERWGIYDAQSRV
                     NPVARSNVCPELAPPAEPCFYAFAFTVVDAGAAPSFVIAGSGEAREGGALYRERTVRY
                     GETSAEAMREKAVFVLGEMQRRLARLGFTWADTSASQVYTVHDLYPFLADEIVPRGAA
                     RAGLTWHYCRPPVRGLEYEMDCRAVAVEHFVE"
BASE COUNT          659 a         1265 c         1216 g          652 t
ORIGIN      
        1 gctcaggcgc cgagcgctgg cgcgggcgta gaaaggtgcc gagcggcagg cggtgccgcg
       61 cttcgcggtt cgcctcgcca gctgcccgtg ggctttgaaa ttgttggcca aaggcatgcc
      121 tcacagcccc atgtatgcgg cgcggatctc gtcgctggcc gcgaggttag cggcggcgcc
      181 ctcggccttg acgcggccga cctcgagcag ataggcgcga tcgaccacct tcagcacctg
      241 ccgcacgttc tgctccacga tcagcacggt atagccttcg ccacggatgc gccgtaccag
      301 gtcgaacacc tggctgacca ccaccggggc caatcccatc gagggttcat cgagcagcac
      361 cagcttgggg ccgctcatca gcgcccgcgc gatggcacac atctgctgct ccccgcccga
      421 gagcgtgccc gccagttggc ccttgcgctc ggcaaggcgc gggaacaagc cgtagacgta
      481 ctcgcgccgt tcggcaaacc gcgcccgcgc cgaggcgatg aaggcgccca tgcgcaaatt
      541 ttcctcgacg ctgagcagcg gaaagagccg acggctctcc ggcacatggg caatgccggc
      601 cgcgacaatg ctgtgcgccc gcatggccaa caagtcgcgg ccttccagcg tcatgcgccc
      661 cgaggtcggc ggcagcagcc ccgaaatcac gcgcaggagc gtggtcttgc cggccccgtt
      721 tgcgccgatc accgccacgg cctcgccggc ggcaacctgc atggagacgc cgaacagcgc
      781 ctggaaaccg gcgtatcccg cgttgatatc gctgagttcc aacatggcct cagtcctggt
      841 ccggcccgag gtagacttcg atgacccggc gattggattg cgccgccttc ggcgcgccat
      901 cgaaaatctt ctcgccatga tcgagcacga cgacgcgatc gaccatccgc atcaatacgc
      961 ccatgatatg ctcgacccaa ataatggtga tgcgcatttc gtcccggatg cggcgcagca
     1021 tcagggccgc ctgctccatt tccgcatgat cgagcccgtt caggctctcg tcggccaaca
     1081 ggagtttggg ccgggtcgcc agggcgcgcg ccagctcgag ctttttcagc gcagccgcgc
     1141 ccagtccatc cgtcgtggtg cgcgcatccg tcggcagccc gacgagggcg agggcttcgc
     1201 cggcctcgcg ccagcaagcg gctcgatcca ccttgccctc ctgcccgaac caggcggaca
     1261 aggcgacgtt ttcgatcaag gtcagattgc ggaacggtcg cggaatttgg taggtgcgcc
     1321 cgatgccctt gtgacagacg tgcgtggccg agaggccggc gatttcctgc tcctccaaca
     1381 ggatcgagcc cttgctcggg gcgtacatgc cggcaatgca gttgaaggtg gtgctcttgc
     1441 ccgagccgtt cggcccgatc agtccgagga tctcaccctg atcgacatcg aacgacacat
     1501 tcgagacggc cgtgaaaccg ccgaatgtct tggtcaggcc ggagactttg aggaacgcca
     1561 cttggcccgc gcgtcgattg gagaaagtcg cgggagccga caggcacgac ggctccccga
     1621 ttcggcgcct gtcagcgcgc gtaggcgtgt cccttcggca gcggcaacac cggatcgacg
     1681 gtgcggatcg ccgtcggcca ggcgatcttg gtatgtccgt cgacgtattg catcacgacc
     1741 ggtgtagagc gctcgttctg ccccgccatt ttcgtgccgg gcggaaagaa tttgaccccg
     1801 taaccctgaa tggtaccgcc gagcgggatg tcggtatcga gcgcggcctt gcgcaaggcc
     1861 tccgcatccc agccgccgta tttcttgatg gctcgcggca gcacatcggt caggaagatc
     1921 caggcctggt tgaaccccat cgaggcgtgc ggcggcacct cgctggcgcc ggtttccgcc
     1981 ttgtagcgct tcaccagctc ggccgtaatc tcgcctacgc ccggctgcag cgtcttggga
     2041 tcgagcagct ggccggccac cggatcgacg ttatagatgt agttgacgtc atccttgaag
     2101 gtttcgcgca gcttgtcgat ctggccatag cccgcgcctt cgccaatcag ggcggagaat
     2161 ttcaagccgg cttccttggc ctggcgcagg aacagcgtga tgtccggatt gtagccggcg
     2221 tgcacgatca catcgggctg ggcgcgccgc agcttggtaa cgagcgcgga gagatcggtc
     2281 gcggtggcgg aatagccctc cttgtgcacg acctggatgc cgagcgcctt gcatcgctcc
     2341 tcgctcgcca cgcccacgcc gaccccgtag ggaccgtcct cgtgaatgat ggcaaccttg
     2401 acgtctttcg gctccttgcc gagcttgccc ttggcgttct cggcgatgaa cgagcaggtg
     2461 gcatcgccgt actgatccga atgaatggtc ggccggaagg cgtaggtcag gttcttgtcc
     2521 ttgaagacgg ccgaggcgac gcacacgttg gcccacagga acttcttggc cgcatcgacc
     2581 ttggcggcca gcggcacgca ctgcgcgctg gagtagacgc ccatcaacag gtcgaccttc
     2641 gcctcgttca gcagccgctc ggcttcgttg atggcaacgt cgaccttgga ttgcgcatcg
     2701 gcatcgatcg ccacgatctt atgcccctcg acgccgcctt tctcgttgat catgtcgatg
     2761 gcgatcttat tgccgataaa ggcgggcttg gaaccgccgc cagcgaacgg gccggtaagg
     2821 tcgtagatcg tgccgatctt gatgtcctgc gccgcggcgc cggtcgccgt caacacagcg
     2881 gccaaacatc cgccggcaat gacaaccggt agggcaagcc tcatgagttt cctccgttgc
     2941 gagctggggc tgggaccttt gcgagccggg tcccgggcgt gaaacgttgg cggcagtgtg
     3001 tgaactgcaa tgggggctgt caaccggaac ccgccctcat tcgacgaaat gctcgaccgc
     3061 caccgcccgg cagtccattt cgtactcgag gccgcgcacg ggcgggcggc agtaatgcca
     3121 ggtgagcccg gcgcgcgccg ccccgcgcgg cacgatctcg tccgccagaa acggatagag
     3181 atcgtgcacg gtataaacct ggctggcgct ggtgtcggcc caggtgaacc ctaatcgcgc
     3241 aaggcgccgc tgcatctcgc ccagcacgaa aaccgccttt tcgcgcatgg cctcggcgct
     3301 cgtctcgccg tagcggacgg tgcgttcgcg atagagcgca ccaccctcgc gcgcctcgcc
     3361 gctgccggcg atgacgaagc tgggcgccgc accggcgtca acaacggtga aggcaaaagc
     3421 gtaaaagcac ggctccgccg gcggagcgag ctcaggacac acgttgctgc gcgccaccgg
     3481 attgacgcgg ctttgagcgt catagatgcc ccacctttcc agggtccccg catagagttc
     3541 attgaaggcg cgaaaggttg cgtcgctgaa gggcgccggt gaacgcagct cgcaggcgca
     3601 gaacgccgtc aagggccggc cgcgggccgt gatgatctcg gcggcacgtt cgaagccgcg
     3661 cgccaacggc aggggatggc gaaagcgcac gcgctcgatg cggaagccgg gcgctgccgc
     3721 cacgccgccc gaatactgaa acaccgcggg aatgaagcga tagccgccgg gcgcgaaaag
     3781 ttcgctcacg cc
//
LOCUS       DASTMT010000629         6603 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_6448066, whole genome shotgun sequence.
ACCESSION   DASTMT010000629 DASTMT010000000
VERSION     DASTMT010000629.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 6603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6603)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6603
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6448066"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..271)
                     /locus_tag="VFR00_14575"
     CDS             complement(<1..271)
                     /locus_tag="VFR00_14575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0160544.1"
                     /translation="MQKAHNLFDLEREKALADGLRDVASELRLIEATDLVAFIRTEQF
                     ANIANLVSSSTELYFKPDTVRFSQSGEVNLTWGGAPSVALDMEFHH"
     gene            complement(282..1364)
                     /locus_tag="VFR00_14580"
     CDS             complement(282..1364)
                     /locus_tag="VFR00_14580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008197007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HEU0160545.1"
                     /translation="MAEHHDKDSKTEEPTEKKLRDAVERGNTPFSKEAGILASLLGIL
                     IISSFFLTSNVGRLADALQRLLDDPGGWALNNGADAVRLFEALGLEVARLLLPVIIVL
                     ASAGLLASFLQNSPRIVFDRIQPQLSRLSLAKGWQRLLGQHAQVEFLKAVLKLSAVCL
                     LGGMTLSAAKHEVSNAIFTEPAALPALIHGMAGRFVATLAVGAVVLVAADLVWSRLFW
                     QRELRMTRQELKEEMKQVDGDPIVKARLRSLARDRLRKRMIAAVPRATMVIANPTHFA
                     VALRYRREEGGAPLVVAKGQDYIALKIREVAEQHGIPVIEDKALARALHKAVEVDRMI
                     PPEFYKAVAEIVIYLYARQAAVRPAV"
     gene            complement(1368..2390)
                     /locus_tag="VFR00_14585"
     CDS             complement(1368..2390)
                     /locus_tag="VFR00_14585"
                     /inference="COORDINATES: protein motif:HMM:NF013838.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliG C-terminal domain-containing protein"
                     /protein_id="HEU0160546.1"
                     /translation="MSVSPQGKSLPASPPLPGPAKAALLLLALGKTRAAKVLRRFDAE
                     ELKQLSRSALALRPVPLGDLDNLIEEFAQRFATGLNFIGSINDVKSLLDDALASEAPG
                     TALEGAPTGSPGVWEVVAKQQPQALQPYLLREHPQTIAVILSKIGSEPAAAVISILPA
                     GLRNTLLARMLNLKSIEADGLDVLEATLREDFMATATAGGAYAEIANILNRLDKAQCD
                     ELIKGLAEKRPDDAKALKRLLFTFAELPLLPAKARGVLLDQVPIERLVLALRGTDAAF
                     QASILSTLASRSKRMVEAELQNPVDVPPAEIAQARRAIVEVVLKMMAKGDVEPPAGEA
                     SEEPAG"
     gene            complement(2406..2831)
                     /gene="fliN"
                     /locus_tag="VFR00_14590"
     CDS             complement(2406..2831)
                     /gene="fliN"
                     /locus_tag="VFR00_14590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019402927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HEU0160547.1"
                     /translation="MTDTAPLKHGSLLQEYAAELTPTPEELAVALGPAAGRASNAGKP
                     RPPEPSALASRNLEMVMRIPVTMKIVLGSATMPVASLVKLGRGAVIPLERRVGEPVDV
                     VVNDRVIARGEVVVVDETTSRFGISLTEIIGPPGNEQPQ"
     gene            complement(2856..3803)
                     /locus_tag="VFR00_14595"
     CDS             complement(2856..3803)
                     /locus_tag="VFR00_14595"
                     /inference="COORDINATES: protein motif:HMM:NF014235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HEU0160548.1"
                     /translation="MLAAGQTPLGAPARRLEASFAARANVAERLPLLALTLERAGEIC
                     AEGLRELGAKPAAITLKSLENGAAESLLGGVDSGLVAGVLHAGKWDARLPVSLRQDTI
                     LALLELAFGGDGSEPADLGTRPLSKIEIRLARRFFARLAQALEQAFADVAPTPFAVEA
                     AEAEPDRDGFQGAHAAVAIAKYHFEWCGRGGEIGLALPQSTLAELRSVLAVVPKKERP
                     RPADPNWVQRIEQEITRSRVVLSAILDERPILLGEVAHLEVGQILPLTATAHSRVYVE
                     CNNERLLCCQLGKSNDVYTLRVDRHVDQEQEFMDEILSR"
     gene            complement(3803..4696)
                     /gene="motA"
                     /locus_tag="VFR00_14600"
     CDS             complement(3803..4696)
                     /gene="motA"
                     /locus_tag="VFR00_14600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008835485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HEU0160549.1"
                     /translation="MTIILGLVITLGCLLGGFVAMGGHVHVIWQPWEYVIICGSALGT
                     FVVANPMKTIKDAGKGILEAFKDAAPKRRQYLDVLGALHSLMRELRSKPRNEVEAHFE
                     DPAQSPVFQKYPTLLANAGLTAFICDYCRLIIIGNARTHEIEALMDEEIQTLRQDQLK
                     SYHSLVSVSEALPALGIVAAVLGVIKAMGALDQAPEVLGHLIGAALVGTFAGIFFSYS
                     VVSPVATKVKTIREKKTRLYVIVKQTLLAFMNGAMPQVAVEHGRKTISAYERPSIDEV
                     ERETLAFAGAAADEVARKEAA"
     gene            4860..5645
                     /locus_tag="VFR00_14605"
     CDS             4860..5645
                     /locus_tag="VFR00_14605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007195841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1217 domain-containing protein"
                     /protein_id="HEU0160550.1"
                     /translation="MSTTISSYQLVAADLPRALATTSAKPTVARDAEYYLAHIQDVKS
                     IDDLLRNQRLFAFAMKAFGLEDMTYAKAFMRKVLSEGVDSKDAFAIKLSDSRYREFAE
                     AFNFKRYGETATVFERTRQGTVDRYVRQVMEEDAGSQNDGVRLALYFQRKAPTLTSAY
                     GILADAALLKVVQTALGLPPAMSNMDIDRQADIISARLDIEDLKDPAKLGKFLTGFAA
                     RWDIANEPAATAAGPGLLFAQPREAGIAGDLLASLQNLRLGGP"
     gene            5651..6376
                     /gene="flgF"
                     /locus_tag="VFR00_14610"
     CDS             5651..6376
                     /gene="flgF"
                     /locus_tag="VFR00_14610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HEU0160551.1"
                     /translation="MQSNLYVSLSAQMSLQKRLETIAHNLANASTAGFRAEEIKFESV
                     LSLAAPDPVAFASAGTSFISRKSGEFVRTDNAFDVAVEGAAWLGINTPTGQVFTRDGR
                     MRMTEAGELVTLNGHAILDAGGAPIRLEPNAGPPRIARDGTITQNERQVGALGLFQID
                     PAAKLSRFENSGVVPDRAATPVLDFSKTGVQQGFIERANVNPVQEITKLIMVQRAYEA
                     VTASIKDAETSLQDAIRSLGSNS"
BASE COUNT         1138 a         2193 c         2161 g         1111 t
ORIGIN      
        1 cgtggtggaa ctccatgtcg agcgccaccg agggcgcgcc gccccaggtc aggttgactt
       61 cgccggattg ggagaacctg acggtgtccg gtttgaagta gagctccgtc gaggagctga
      121 ccaggtttgc aatgttggcg aactgttcgg tgcgaatgaa ggcgacgagg tcggtggcct
      181 cgatcagccg cagctcggat gcgacgtcgc gcagaccgtc cgcaagcgcc ttttcgcgtt
      241 cgagatcgaa tagattgtgc gccttttgca tgccttgctc tttacacggc aggccttacc
      301 gcagcctgac gcgcatagag gtagatcacg atttccgcga cggctttgta gaattccggc
      361 ggaatcattc ggtccacttc cacggcttta tgcagcgcgc gcgccaacgc tttgtcctcg
      421 atcacgggaa tgccgtgctg ttcggcaact tcgcgaattt tcagggcaat gtagtcctgg
      481 cccttggcta ccacgagcgg ggcgccgccc tcttcgcgcc ggtagcgcag ggccacggca
      541 aagtgcgttg gattggcgat caccatggtg gcgcgcggga cggcggcgat catgcgcttg
      601 cgcaggcgat cgcgcgccaa cgagcgcagg cgcgccttga cgatcgggtc gccgtccacc
      661 tgcttcatct cttccttgag ctcctggcgc gtcatgcgca gctcgcgctg ccagaagagg
      721 cgcgaccaga cgaggtcggc ggccaccagg accaccgccc cgacggcgag cgtcgccaca
      781 aagcgcccgg ccatgccatg gatcagcgcc ggcagcgccg ccggctccgt gaagatggcg
      841 ttgctgacct cgtgcttggc cgcgctcagc gtcatgccgc ccaggaggca gacggcggaa
      901 agcttgagga ccgccttcag gaattcgact tgggcatgct gtcccagcag ccgctgccag
      961 cccttcgcca gcgacagccg cgaaagctgc ggctgaatgc ggtcgaacac gatgcggggg
     1021 gaattctgca gaaacgaggc caagagaccg gcgctggcca acacgatgat gacgggcagc
     1081 agcagccgcg ccacttccag ccccagagcc tcgaacagcc gaaccgcatc ggcaccgtta
     1141 ttgagcgccc agccgccggg atcatcgagg agccgctgca gggcgtcggc cagacgaccg
     1201 acattgctgg tgaggaaaaa tgacgaaatg atcagaatgc ccaacagcga ggcgaggatg
     1261 ccggcttcct tggaaaaggg cgtattgccg cgctcgacgg cgtcccgcag ttttttttcg
     1321 gtcggctctt cggtcttgct gtccttgtcg tggtgctccg ccatggccta gcccgccggc
     1381 tcctcgctgg cctcgcccgc gggcggctcg acgtcgccct tcgccatcat cttgagcacc
     1441 acctcgacga tggcgcgccg cgcctgcgcg atctcggccg gcggcacgtc gacagggttt
     1501 tgcagctccg cctcgaccat gcgcttggag cgcgaggcga gcgtggaaag gatgctcgcc
     1561 tgaaaggcgg catcggtgcc gcgcaaggcc agcacgagcc gctcgatcgg cacctgatcg
     1621 agcagaacgc cgcgcgcctt ggcgggcagc aggggcagct cggcaaaggt gaagagcagc
     1681 cgcttgaggg ctttggcgtc gtcgggacgc ttctcggcca agcccttgat cagctcgtcg
     1741 cattgagcct tgtccaggcg attgaggatg ttggcaatct ccgcataggc gccgccagcg
     1801 gtggccgtcg ccatgaagtc ttccctgagc gttgcctcga ggacatccaa gccatcggct
     1861 tcgatgcttt tgagattgag catgcgggcc agcagcgtgt tgcgtaggcc agccggcaag
     1921 atgctgatga ccgcggccgc gggctcggag ccgattttgg agagaatgac ggcgatggtc
     1981 tgcgggtgct ccctgagcag gtaaggctgc agcgcctggg gttgctgctt ggcgacgacc
     2041 tcccataccc ccggcgagcc ggtcggcgcg ccctccaggg ccgtgcccgg cgcctcgctg
     2101 gcgagagcat cgtcgagcag acttttgacg tcgttgatcg agcctatgaa gttgagaccg
     2161 gtggcaaagc gctgggcgaa ctcttcgatg agattgtcga ggtcgcccag aggcaccggc
     2221 cgcagcgcca gcgcgcttct gctcagctgc ttcagctcct cggcatcaaa gcgccgcagc
     2281 accttggccg cccgtgtctt gccgagcgca agcagcagca aggcagcctt ggccggccct
     2341 ggcaggggcg gggaggctgg taaactcttg ccttgcgggc tgacgctcat gacccggccg
     2401 gccgcctatt gcggctgctc gttgcccggc ggaccgatga tttcggtcag cgagatgccg
     2461 aagcgcgagg tggtctcgtc gacgacgacg acctcaccgc gggcgatgac gcggtcgttc
     2521 accaccacgt cgacgggctc gccaacccga cgctcgagag gtatgacggc cccgcggccg
     2581 agcttgacca ggctcgccac cggcattgtt gccgagccga gcacgatctt catggtcacg
     2641 gggatgcgca tcaccatttc gaggttgcgc gaggcgagcg cgctcggctc gggcgggcgc
     2701 ggcttgccgg cattgctggc gcggccagcc gccggtccca gcgccaccgc cagctcttcc
     2761 ggcgtcggcg tcagctcggc cgcatactcc tgcaacaacg atccgtgttt gagtggcgcg
     2821 gtgtccgtca tgtccttttt accttgcaag atgcctcagc ggctcaagat ctcgtccatg
     2881 aattcctgtt cctggtcgac gtgccgatcg acgcgcagcg tgtagacatc gttcgatttg
     2941 ccgagctggc agcacagcag tctctcgttg ttgcactcca cgtacacgcg gctgtgcgcg
     3001 gtcgccgtca ggggcaggat ctggccgacc tcgagatgcg ccacttcgcc caacaggatc
     3061 gggcgctcgt ccaaaatcgc gctcagcacc accctggagc gggtgatctc ctgctcgatg
     3121 cgctggaccc agttcggatc ggccggccgc ggccgttcct ttttggggac caccgccagc
     3181 acgctcctga gctcggccag cgtcgactgc ggcagcgcga ggccgatttc cccgccccgc
     3241 ccgcaccatt cgaagtgata cttggcgatt gccaccgccg catgggcccc ttggaagccg
     3301 tcgcggtcgg gctcggcctc ggcggcctcg accgcgaacg gcgtcggcgc cacgtcggcg
     3361 aacgcttgct ccaaggcctg cgccaggcgg gcaaagaagc gccgcgccaa gcggatctcg
     3421 atcttcgaca ggggccgggt gccgaggtcg gcgggttcgc tgccatcccc accgaaagcc
     3481 agttcgagca gcgccagaat tgtatcctgg cgcaagctga cgggcagccg ggcgtcccat
     3541 ttgccggcgt gcagcacacc ggccaccaga ccactgtcga caccccccag taggctttcc
     3601 gccgcgccgt tttcgaggct tttgagcgtg atggcggcgg gcttggcgcc gagctcgcgc
     3661 aagccctcgg cgcagatctc tcctgcgcgc tcgagcgtca gcgccaacag cggcaggcgt
     3721 tcggcgacgt tggccctggc ggcgaagctc gcctcgagcc gccgcgcggg tgcgccgagc
     3781 ggtgtttgcc ccgccgccag catcaggcgg cctccttcct ggcaacctca tcggccgccg
     3841 cgccggcgaa ggccaaagtc tcgcgttcga cctcgtcgat ggacgggcgc tcgtaggccg
     3901 agatcgtctt gcgcccgtgc tcgaccgcca cctgcggcat ggcaccgttc atgaaggcca
     3961 gcagcgtctg cttgacgatc acgtagaggc gggtcttttt ctcgcggatg gttttgacct
     4021 tggtggcgac cggcgaaacc accgaatagg aaaaaaagat gccggcgaac gtgccaacca
     4081 gggccgcgcc gatcaggtgg cccagcactt ccggcgcctg atccagcgca cccatggcct
     4141 tgatgacacc cagcacggcg gcgacaatgc cgagcgcggg caaggcctcg gacaccgaga
     4201 ccagcgaatg gtaggacttc agctggtcct gccgcagggt ctggatctcc tcatccatca
     4261 gcgcttcgat ctcatgcgtg cgggcgttgc cgatgatgat cagccgacag tagtcgcaga
     4321 tgaatgccgt gagcccggcg ttggccagca gggtcgggta cttctggaac accgggctct
     4381 gcgcgggatc ctcgaagtgg gcctcgacct cattgcgggg cttgctgcgc aactcgcgca
     4441 tcagcgaatg caacgcgccc agcacatcga ggtattggcg gcgcttcggg gctgcgtcct
     4501 tgaaagcctc caggatgccc ttgccggcgt ccttgatggt tttcatggga ttggcgacga
     4561 cgaaggtgcc gagcgccgaa ccgcagatga tcacgtattc ccagggctgc cagatgacgt
     4621 ggacatggcc gcccatggcc acgaagcccc caagcaaaca gcccaaggtg atgacgagac
     4681 cgaggatgat ggtcacgggg gtgcggctcc gctgattttc cagccccaag ctaaaccgcg
     4741 tgcgccttac gcgaggctgg cgccccggcc tcgggcgcgc gcacgcggct tgccgcgcca
     4801 gcttcgggca agccgccttt gccaagctcc gccgatcctg gggagggaga cctcaagttg
     4861 tgtcgacgac gatcagcagc taccagctcg tggcagccga tctgccgcgc gcgctcgcca
     4921 cgacgagcgc caaaccgacg gtggcgcgcg atgccgagta ctatctggcg cacatccaag
     4981 acgtgaaatc gattgacgac ctcctccgca atcagcgcct gttcgccttt gccatgaagg
     5041 cctttggtct cgaggatatg acctatgcca aggcctttat gcgcaaggtc ttgagcgagg
     5101 gcgtcgacag caaagatgct ttcgccataa agctcagcga ttcacgctac cgggaatttg
     5161 ccgaggcctt caatttcaag aggtacggcg aaacggcaac cgtgttcgaa cgcacgcgcc
     5221 agggcacggt cgatcgctac gtgcgccagg tcatggagga ggacgcaggc agccagaatg
     5281 acggcgtgcg gctggccctc tacttccagc gcaaggcgcc aacactcacc agcgcctacg
     5341 gcatccttgc cgacgccgcc ctgctgaagg tcgtccagac ggcgctcggc ttgccgccgg
     5401 ccatgtccaa catggacatc gaccgccagg ccgatatcat ctcggcgcgc ctcgacatcg
     5461 aggacctgaa agaccccgcc aagctcggca aattcttaac cggctttgcg gcgcggtggg
     5521 acatcgccaa cgagccggcg gcgacagcgg ccggcccagg cctgctgttc gcacaacccc
     5581 gcgaggccgg gatcgccggc gatctgctcg cgagcttgca aaacctgcgg ctcggaggcc
     5641 cttaaacgcc atgcaatcca acctctatgt gtcgctgtcg gcgcagatgt ccctgcaaaa
     5701 acggctggag acgatcgcgc acaacctcgc caacgccagt accgccggct tccgcgccga
     5761 agaaatcaaa ttcgagagcg tgctgtcgct ggccgcgccc gatccggtgg ccttcgcctc
     5821 ggcgggaacc agcttcattt cgcgcaaatc gggcgaattc gtcaggaccg acaacgcttt
     5881 cgacgtggcc gtggagggcg cggcctggct tggcatcaac acgcccacgg gccaggtctt
     5941 cacgcgcgac gggcgcatgc gcatgaccga ggcgggtgag ctcgtgacgc tgaacggcca
     6001 tgccatcctg gacgcaggcg gcgctccgat ccggctcgaa cccaatgccg ggccaccgcg
     6061 gatcgcgcgc gacggcacca tcacccagaa cgagcgccag gtcggtgcgc tcggcctgtt
     6121 tcagatcgat ccggccgcca agctcagccg cttcgagaac tccggtgtgg tgcccgaccg
     6181 ggcggccacg ccggttctgg atttcagcaa gaccggcgtc cagcagggct tcatcgagcg
     6241 cgccaacgtt aatccggtgc aggagatcac gaagttgatc atggtgcagc gcgcctacga
     6301 ggccgtgacg gcctcgatca aggacgcgga aacctccttg caggatgcga tccgatcgtt
     6361 gggctcgaat agctagctcg ccgcaggccg gcattccccc gcagcgtggc aagctgcgaa
     6421 ccgcaacgga gcgccgccag cccggcggcg gggaatgcgg cccggccaag ggccccaaga
     6481 ggtctgagag gaaggagacg accagaggtg aagagcccac tcgatccaag gctcgcgctt
     6541 gccccaagcg ccgaggttgg cgccgaggtt ggcgccgcgg cggctggccc ggcgagcggc
     6601 cac
//
LOCUS       DASTMT010000630         2247 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_3867086, whole genome shotgun sequence.
ACCESSION   DASTMT010000630 DASTMT010000000
VERSION     DASTMT010000630.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2247)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2247
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3867086"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(114..1277)
                     /locus_tag="VFR00_14615"
     CDS             complement(114..1277)
                     /locus_tag="VFR00_14615"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEU0160552.1"
                     /translation="MSSITNGRAGWVRLAVNRFAKAPKVLGVAVLGVAGLTAGCKGES
                     AAKPEAVRPVKVAIVEAAPEVRTLSYAGVVRPRIESARGFRVSGKIIERLINVGDRVE
                     VEQPIARLDETDLKLAENSARAAVAAAASRRDVASDNLERAKVLLPKAIISQAAYDTR
                     RNELDAAVASLDSAQAQLRQAVNAVSYTSLTADKAGIVTAVSAEPGQVVSAGQPIVTV
                     ADAGETEIAVAVPEQDAPRLALGQWAKVTLWAGSGLSVDGRVREIAGQAEPASRTYAV
                     RIAVATPPQAMRLGMTATVSLRLEHETAPMLLPLTALTESSDNAAVFVVDRSQQVVRR
                     TIVTLAGTSEDGVRIASGLSAGDMVVTAGVQFLRDGMRVRLLEDRAQAPGNRI"
     gene            1431..2099
                     /locus_tag="VFR00_14620"
     CDS             1431..2099
                     /locus_tag="VFR00_14620"
                     /inference="COORDINATES: protein motif:HMM:NF024701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEU0160553.1"
                     /translation="MGRLTVKQGQAQGQARGQAKARYHHGELREALIRATRKLVEERG
                     AENFTLADACRVAGVTTAAPYRHFQGKQEILAEIASRGFDELRSRSMAVIAEKGEGSL
                     DGIIALGQAYVAFAVEETAVFRLMFGQEPSLKKAQHVVGTGHDCFVHLIDQIALYCKH
                     NKVRGDAQEIALKLWTFVHGAASLLIDQDYDAVAPGIDVNKLIAGATPGLLAAAPPRQ
                     ARRK"
BASE COUNT          362 a          773 c          727 g          385 t
ORIGIN      
        1 aggttgaagg tcgcggaggg cagtctggtg gtggtgtttg acatggctgt catcgctcca
       61 gagccgtcga ggggcgcacc cctttgcggc ggcaaatatg ggtttagcgg cagctaaatc
      121 cggttgcctg gggcctgcgc ccggtcttcc agcaggcgga cacgcatgcc gtcgcgcaag
      181 aattgcaccc ccgccgtcac gaccatgtcg ccggcgctca aaccgctggc gatgcgcacg
      241 ccatcctcgg acgtacccgc cagcgtcacg atggtgcgac gcaccacctg ttggcttcga
      301 tcgacgacga acaccgcggc gttgtcgctc gactcggtca aggccgtgag cggaagcagc
      361 atcggcgccg tctcgtgttc gaggcgcagg ctcacggttg ccgtcatccc caagcgcatg
      421 gcctgcggcg gcgtggcgac ggcaatgcgc acggcatagg tgcgcgatgc cggttccgcc
      481 tggccggcga tttcgcgcac gcggccatca acgctgaggc cagaccctgc ccacagcgtc
      541 accttcgccc actgtccgag cgccagccgc ggcgcatcct gctcgggaac cgccacggcg
      601 atttccgtct caccggcatc ggccaccgtg acgatgggct gaccggcgct gacgacttgg
      661 cccggctcgg cgcttactgc cgtcacgatg ccggccttgt cggccgtcaa gctcgtgtag
      721 gacaccgcgt tcaccgcttg gcgcagttgc gcttgcgcac tgtccagcga cgccacggcg
      781 gcatcgagct cgttgcgcct ggtatcgtag gcggcctgcg agatgatggc cttgggcagc
      841 agcaccttgg cccgctccag attgtcgctg gcgacatcgc gccgactggc cgcggccgcc
      901 accgccgctc tggcgctgtt ctccgccagc ttgaggtcgg tctcatccag gcgggcgatc
      961 ggctgctcga cctcgacgcg gtcgccgaca ttgatcaggc gctcgatgat cttgcccgag
     1021 acccgaaacc cgcgcgcgct ttcgatgcgc gggcgcacca cgccggcata gctgagcgtc
     1081 cgaacctcgg gcgccgcctc gacgatggcg accttcaccg gccgcaccgc ttctggcttg
     1141 gccgcgcttt cgcccttgca gccggcggtg aggccggcaa cgccgagcac ggcgacgccc
     1201 aacaccttgg gcgccttggc gaaccggttg acggcgagcc ggacccaacc agcgcgcccg
     1261 tttgtgatgc tgctcatgct catctccctt tttttcgccc cctatcggca aagtcggccg
     1321 gcgccgagct tgcgatgtga gcgcgactaa catatgttag cagcgctgac atcgcaaagt
     1381 gttagcgacg ctaacttttc aactcatcgt gaagcggtca tgctcgggta atggggcgac
     1441 tcacggtcaa gcaggggcag gcccagggcc aagcccgcgg gcaggcaaag gcccgttatc
     1501 accacggcga actgcgcgag gcgctgatcc gtgccacgcg caagctcgtg gaggagcgtg
     1561 gcgccgagaa ttttacgctc gccgatgcct gccgcgtggc gggcgtcacc acggcggcgc
     1621 cctaccggca ttttcagggc aagcaggaga tcctggccga aatcgcctca cgcggctttg
     1681 atgagctgag gagccggtcg atggccgtca ttgccgagaa aggcgagggc agcctcgacg
     1741 gcatcatcgc cctgggccag gcctatgtgg cctttgccgt cgaggaaacc gccgtcttcc
     1801 gtctcatgtt tggccaggag ccctcgctca aaaaggcgca acacgtggtg ggcaccggcc
     1861 acgattgctt cgtccatctc atcgatcaaa tcgcgcttta ctgcaaacac aacaaggtgc
     1921 gcggcgacgc ccaggagatc gccctgaagt tgtggacatt tgtgcacggc gcagcctcgc
     1981 tcttgatcga tcaggactac gacgcggtgg caccggggat cgacgtcaat aagctgatcg
     2041 ccggggccac tccgggcttg ctcgccgccg cgccgccgcg ccaggctcgg cgaaaataaa
     2101 gcgtccctct cgtcggcccg cgcccgggcc atggccagcc gtgctgccgc cggcgctcgc
     2161 ttggccaagg acaggacagg cgtgccttga cgcctttgcg gccctgagcg cgcacaagct
     2221 cgcaagcaga aggctcagct cctcgcg
//
LOCUS       DASTMT010000631         1633 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_4865109, whole genome shotgun sequence.
ACCESSION   DASTMT010000631 DASTMT010000000
VERSION     DASTMT010000631.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 1633)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1633)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1633
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4865109"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..233)
                     /locus_tag="VFR00_14625"
     CDS             complement(<1..233)
                     /locus_tag="VFR00_14625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013165026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU0160554.1"
                     /translation="MKQVEVAVIGTGWCGGIRAETLARSALVDKLHVCEIRPERLAEI
                     AKLTNAASATLDYRDIVANPRIAAVYVSTTPEGT"
     gene            551..1525
                     /locus_tag="VFR00_14630"
     CDS             551..1525
                     /locus_tag="VFR00_14630"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEU0160555.1"
                     /translation="MDRRRFIAGGAASAAALATHAAGAQETYPTRAITIINPFPPGGA
                     ADVVARPFAALLEPILKQPVVIETKAGAAGQVGAQFAASAVPDGYTLLVHIVSISGFA
                     EVDKVFGRQPKFTRADFIPIARFTEGPMVLLVNEQTPYKSLAELVEDAKKRPNQIIFS
                     SSGLYGALHLPTALFAKAAGIEMRHLPTNGGGPALTALLGNNSQVLVSAVAAANAHIK
                     AGKVRALACFSHKRMQSLPDLPTLKELGFDVEFSLWVGLFAPKGTPDAIIAKLREETK
                     KAAASEQFAKAIDNIGDVLAYQDQPEFAKFWDEDARRVEDAVRSIGKV"
BASE COUNT          269 a          542 c          544 g          278 t
ORIGIN      
        1 gtaccttccg gcgtggtgga gacgtagacg gccgcgatgc ggggattggc cacgatgtcg
       61 cggtagtcga gggtggcgct ggcggcgttg gtgagctttg caatctcggc cagacgctcg
      121 gggcggatct cgcacacatg cagcttgtcg accaacgccg agcgcgccaa ggtctcggcc
      181 cgaatgccgc cgcaccatcc cgtgccgatc acggccactt ccacttgctt catcgcctcc
      241 tccttggctt ccggcgagtt agccgctgcg ctgagccttt gtccagggcg ccgcgcggga
      301 catttgggcc ctttggtggc cccgtttggc gggcccgatt ggcaacggca ggccgggctg
      361 gggcgccggc tgctgccggc ggcaagcgct tggcgcgccc gaccccggct ggcgcagtcc
      421 ggcgctcaca cctttggcgt ccttgaccag cctttgttgg catcagtagc attccccgac
      481 gccgcgctgc cgccgggcag aaccttggcg gccaacccgt agctgaagac caagacgtga
      541 gggaggaagc atggataggc gcaggttcat tgctggcggg gccgcctcgg ccgcggccct
      601 ggcgacgcac gctgccggcg cgcaagagac ctacccgacc cgggccatca ccatcatcaa
      661 tccctttccc cccggtggcg ccgccgacgt ggtggcgcga ccgttcgcag ccctgctgga
      721 gccaatattg aagcagccgg tcgtgatcga gaccaaggcc ggtgccgccg gccaggtcgg
      781 ggcccagttt gccgccagcg cggtgccgga cggctacacg ctgctcgtgc atatcgtttc
      841 gatttccggt tttgccgaag tcgacaaggt gtttggtcgc cagcccaagt tcacgcgcgc
      901 cgacttcatc ccgattgccc gcttcaccga ggggccgatg gtgctcctcg tcaacgagca
      961 aacgccctat aagagtttgg cggaattggt cgaggacgcc aaaaagcggc cgaaccagat
     1021 catcttcagc tcgtcggggc tgtacggtgc cctgcacctg ccgacggcgc tgtttgccaa
     1081 ggcggcgggc attgaaatgc gccacctgcc caccaacggc ggcggcccgg cgctgaccgc
     1141 cctgctcggc aacaattcgc aagtgctcgt atccgcggtg gcagccgcca atgcccacat
     1201 caaggccggc aaggtgcgcg cgctcgcctg cttctcgcac aagcgtatgc aatcgctgcc
     1261 ggacctgccg accctcaagg aactcgggtt cgacgtcgag ttttccctct gggtcggcct
     1321 gtttgccccc aagggcacac ccgatgcgat catcgccaag ctgcgtgagg aaaccaagaa
     1381 ggcggcggcc agcgagcaat tcgccaaggc catcgacaac atcggcgacg tgctggccta
     1441 ccaagaccag ccggagttcg caaagttctg ggacgaggac gccaggcgcg tcgaagacgc
     1501 ggtgcgatcg atcggcaagg tttaaggcga cgggatcaac aggctcaagc cacgaggggg
     1561 gcgcatgcag gtgcgtaggg accatgttgc cggcgcggtg atgctgctcg ccggtgtgct
     1621 ggtgctggcg gca
//
LOCUS       DASTMT010000632         2177 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_4535226, whole genome shotgun sequence.
ACCESSION   DASTMT010000632 DASTMT010000000
VERSION     DASTMT010000632.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2177)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2177)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2177
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4535226"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            102..872
                     /locus_tag="VFR00_14635"
     CDS             102..872
                     /locus_tag="VFR00_14635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0160556.1"
                     /translation="MHKALVIVPILGWLGVGALLHQTREWPASKPDVGVVVVVPPRPA
                     AAAPAAQLPRAADRAQLTRELHKELKRVGCYQGEIGSAWGNASRQAMRRFTEAVNAKL
                     PIEEPDLVLLRLAQGQAQRVCSCPAAAGEASASCATAAGPAEAGKTGGNEARLADQAD
                     PQRATPLIVGAAATTAAAATALARPETGSSNARLASAPTANAEETRAARQSGPTPPAD
                     VYASRRRTSARRAESRPPAVVQSLMRDMQRALGSFGIR"
     gene            1009..1311
                     /locus_tag="VFR00_14640"
     CDS             1009..1311
                     /locus_tag="VFR00_14640"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018429822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU0160557.1"
                     /translation="MSTPEPTITREDSATRGRYVARLAGHAGEGELAYSRTSKTLIIA
                     EHTAVDDALRGKGIALALVRRLVEDARAEGFKVYPLCPYVNAERRKHPDWADVFQD"
     gene            1428..1799
                     /locus_tag="VFR00_14645"
     CDS             1428..1799
                     /locus_tag="VFR00_14645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0160558.1"
                     /translation="MATLRLLLALSLIALGTTGAAFAISGYFDPRQWPQEAKGPAEAG
                     TSKLDMGAMKVRQRFVAIEAKAKPAAAAGASKTAPPAPAKATTAVAKAPAKKLPPAKD
                     GKEGNVQQAAAWWPLANLFKN"
     gene            complement(1802..>2177)
                     /locus_tag="VFR00_14650"
     CDS             complement(1802..>2177)
                     /locus_tag="VFR00_14650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEU0160559.1"
                     /translation="LKFVADVMHTGDRLPLVAEDKIMSEAIVIMTQKSFGCLGVIDAK
                     GRLVGMITDGDLRRHMGAGLLRQRTQDIMTRRPNTLAPNTLASAALEQFNALKRTQMF
                     VVDKGKPVGVLHVHDLLRAGVA"
BASE COUNT          354 a          765 c          729 g          329 t
ORIGIN      
        1 gccgcgccgc tcggccgttg aaatcgcgcg ccgctgcctt cctttggccc taagtcggcg
       61 cttttctggg ctggactgtg ttagcggcag ggggccgcgc cttgcacaaa gcgctggtga
      121 tcgttccgat tttgggctgg ctcggtgttg gcgcgctgct gcatcaaacg cgcgagtggc
      181 cggccagcaa gcccgatgtc ggcgtcgtcg tggtcgttcc gccacgcccg gcggccgccg
      241 ccccggccgc gcaactgccc cgtgccgccg atcgtgccca gcttacgcgc gaactccaca
      301 aggagctgaa gcgggtgggc tgctatcagg gcgagatcgg cagcgcctgg ggcaacgcct
      361 cgcgccaagc catgcggcgc ttcaccgagg ccgtcaatgc caagctgccg atcgaggagc
      421 cggacctcgt gctgttgcgg ctggcgcagg gccaggcgca gcgcgtgtgc tcttgccctg
      481 cggcagcagg tgaggcgagc gcgtcctgtg ccacggccgc gggccccgcc gaagccggca
      541 agaccggcgg caacgaagcg cgcctggccg accaggccga cccgcagcgc gcaacgcctt
      601 tgatcgtggg ggccgcggcg acgacggccg cagcggcaac ggccctggcg cggcccgaga
      661 ccggctccag caacgcccgg ctcgccagcg cacccacggc caacgcggag gagaccaggg
      721 cggcgcggca gagcgggcca acgccgcccg ccgacgtcta tgcctcgcgc cggcgcacga
      781 gcgcacgccg cgccgagtcc cgcccgccgg ccgtcgtgca atcgctgatg cgcgatatgc
      841 agcgtgcgct ggggtcgttt gggatccgct agcggcggca gctggcgagc gcaggcgctt
      901 tcagggcctt cggcattgcg tgcgccccga cccaagccta catgtggcgt ggggatgccg
      961 tcttgcggcg caccctcgat ctctcactgc ctgatcaggt cagcgcccat gagcacaccg
     1021 gagccgacga tcacgcgcga ggacagcgcg acgcgtggcc gctacgtggc gcgccttgcg
     1081 ggtcatgccg gcgagggcga actcgcctat agccgcacca gcaagaccct cattatcgcc
     1141 gagcacaccg ccgtcgacga cgctttgcgc ggcaagggca ttgcgctggc gctggtacgc
     1201 cggctggtcg aggatgcgcg cgccgaaggc ttcaaggtct atccgctctg cccctacgtc
     1261 aacgccgagc gccgcaagca tcccgattgg gcggacgtgt ttcaggatta agcggcacct
     1321 cggccgcaga gcttccacat tttgccccta ggctccgcgg aacgtgcggg gtggtattgc
     1381 gtggacgttt cgtcggggaa agtccgcgcc cgccggattg aacccacatg gcaacgctca
     1441 gattgctgtt ggccctcagc ctcatcgccc tcggcaccac cggggcagcc tttgccatca
     1501 gcggctactt cgacccgcgc cagtggccgc aagaggcaaa aggcccggcc gaggccggca
     1561 ccagcaagct cgacatgggc gccatgaaag tgcggcagcg cttcgtcgcc atcgaagcca
     1621 aggcgaagcc ggcggccgcc gctggcgcca gcaaaactgc gccgcctgca cccgccaaag
     1681 ccacgaccgc ggtcgccaaa gccccagcga aaaagctgcc tcccgccaag gatggcaagg
     1741 agggcaacgt gcagcaggcc gccgcctggt ggccgctcgc caatctcttc aagaattaac
     1801 gtcaggccac gccggcgcgc aataggtcgt gcacgtgcaa aacgccgacc ggcttgcctt
     1861 tgtcgacgac gaacatctgc gtgcgcttta gcgcgttgaa ctgctcgagc gcggcggagg
     1921 ccaaagtatt gggcgccagc gtgttgggcc tgcgcgtcat gatgtcttgc gttctttgcc
     1981 gcaacagccc agcccccatg tgtcgccgca agtcaccgtc cgtgatcatg ccgaccaggc
     2041 gacccttggc atcgatcaca ccgagacagc cgaagctctt ttgcgtcatg atgacgatcg
     2101 cctcggacat gatcttgtct tccgcgacca gcggcagacg atcgccggtg tgcatgacgt
     2161 cggcgacgaa cttgagg
//
LOCUS       DASTMT010000633         2039 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_5518775, whole genome shotgun sequence.
ACCESSION   DASTMT010000633 DASTMT010000000
VERSION     DASTMT010000633.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2039
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5518775"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..305)
                     /locus_tag="VFR00_14655"
     CDS             complement(<1..305)
                     /locus_tag="VFR00_14655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0160560.1"
                     /translation="MSYTLSQLAADIHETLKADNGPKGKAKAATLVSRALTDQAFIAS
                     ELKERAPGANPREILYEDPELGFCICGHVYDGPANGSPHDHGSSWAIYGQATGVTEMT
                     "
     gene            478..1017
                     /locus_tag="VFR00_14660"
     CDS             478..1017
                     /locus_tag="VFR00_14660"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU0160561.1"
                     /translation="MLLAIPKWLSVAAAALTVLAFTPAGVLAVGDDPPKPSSNQPKPK
                     SKSKPKPKSKSKDSRLNQDEIYSLGYWQAKRGEYAAALATLRSAPNAADPRVETMIGF
                     SLRKLGQVDEAMAYYNRVLANYPERTTTRQYLGEAFLQIGAPERAKEQLAEIAKRCGV
                     ACEDYQLLAIEIAKYEQAG"
     gene            complement(1025..>2039)
                     /locus_tag="VFR00_14665"
     CDS             complement(1025..>2039)
                     /locus_tag="VFR00_14665"
                     /EC_number="1.11.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF015131.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HEU0160562.1"
                     /translation="LRVVATAVLLMAAAAGAAWPQAPWRPEPAWRQLFRRPAEPPAPA
                     DNAMTAAKVALGARLFSDPRLSGAGQRSCASCHRPVLAFSDGLRRARALTGASLARNT
                     PALWNLAWSEAYFWDGRAPSLEAQVRMPIVAADEMAGDWPSILARLNADPDLLAAFQA
                     AFPGEATIAQDGVAKALASYVRSLVSPRTRFDAWIEGDDGALKANEVRGFRLFTGKAG
                     CVLCHVGWRFTDDRFHDIGLPGNDAGRGAVAGGTPGLPAFKTPSLRELASSAPYMHDG
                     SRPTLAAVVAHYADTFLKRPSLAPSIRRLHLTAAERADLIAFLGTLSSAGSPAQPKSA
                     GHP"
BASE COUNT          361 a          674 c          696 g          308 t
ORIGIN      
        1 gtcatttcgg taacgccggt cgcctgtccg tagatggccc aactggagcc gtggtcgtgc
       61 gggctgccat tggccggccc gtcgtaaaca tgcccgcaaa tgcaaaatcc gagctcgggg
      121 tcctcgtaga ggatttcgcg cggattggcc cccggcgcgc gctccttgag ctcagaggca
      181 ataaaggcct gatcggttaa cgcccgggac acgagggtgg cggccttcgc cttacccttg
      241 ggaccgttgt cggcctttaa ggtttcatgg atgtcggctg cgagctggct gagggtgtag
      301 gacatcgatg cgatccttgg tcagcgccaa tcgtgacaaa acttagcaag agccgcggcc
      361 ggccgccagt cggcgccgcg ggcgggtctt ttcacgcggc cgtgactgcc actttcgccc
      421 gccctcgtat aatctttttc agggtgcaga aagcatcaga ggacggagga gaaaaatatg
      481 ctgcttgcca ttcccaaatg gctgtcggtc gccgcggctg ccctaacagt gctagccttc
      541 acgccggcgg gcgttcttgc cgtcggtgac gatcctccca aaccgtcctc caaccagccc
      601 aaaccgaaat cgaaatccaa acccaagccc aagtccaaga gcaaggacag ccggctcaat
      661 caggacgaga tttacagcct gggttactgg caggcaaagc ggggcgaata cgcggcggcg
      721 ctggcgactt tgcgctcggc gccgaatgcg gccgacccgc gcgtggagac catgatcggc
      781 ttcagcttgc gcaagcttgg gcaagtcgac gaggccatgg cctactacaa ccgcgtcctg
      841 gccaactatc ccgagcgcac caccacgcgc cagtatttgg gcgaagcctt cctccaaatc
      901 ggcgcgccgg agcgggccaa ggagcagctg gctgagatcg ccaagcgctg cggcgtcgcc
      961 tgcgaggact accagctcct tgccattgag atcgccaagt acgaacaggc cggctgagcc
     1021 ggcatcaggg atgccccgcc gacttgggtt gggcggggct ccccgcgctc gacagcgtgc
     1081 cgaggaaggc gatgagatcg gcgcgctcgg cggccgtgag gtggaggcgg cggatgctgg
     1141 gcgccagcga cggtcgcttc aggaaggtgt cggcgtagtg cgccacgacc gccgccagcg
     1201 tcggccgtga gccgtcgtgc atgtagggcg cgctcgaggc gagctcgcgc aaactcggcg
     1261 tcttgaaggc cggtagaccc ggtgtgccgc ccgctaccgc cccacgtcca gcatcgttgc
     1321 ccggcaggcc gatgtcgtgg aagcgatcgt cggtgaagcg ccagcccaca tggcagagca
     1381 cgcagccggc cttgccggtg aagagccgaa agccgcgcac ctcgttggcc ttgagcgcgc
     1441 cgtcgtctcc ttcgatccag gcatcaaagc gcgtgcgggg agaaacgagc gaacgcacgt
     1501 agctggccag cgccttggcc acgccgtcct gcgcaatcgt cgcctcgccg ggaaaggccg
     1561 cctggaacgc cgccagcaga tcagggtcgg cgttgaggcg tgcgaggatg ctgggccaat
     1621 ccccggccat ctcatcggcg gcgacgatcg gcatgcggac ctgggcttcc agcgagggcg
     1681 cacgcccgtc ccagaagtag gcctcgctcc acgccaggtt ccaaagcgca ggggtgttgc
     1741 gggcaaggct ggcgcccgtc agtgcgcggg cccgccgcag cccgtcgcta aaggcaagca
     1801 ccggccgatg acaactggca cacgagcgct ggccggcccc cgacaagcgc ggatcgctga
     1861 agagacgcgc gccgagcgcg accttggcgg ccgtcatagc attgtcggcc ggcgcgggcg
     1921 gctccgccgg ccgccgaaag agctgacgcc aggcaggctc cggccgccag ggtgcctgcg
     1981 gccatgcggc gccggcggcc gcggccatga gcagcacggc cgtggcgacg acccggagc
//
LOCUS       DASTMT010000634         1599 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_5792084, whole genome shotgun sequence.
ACCESSION   DASTMT010000634 DASTMT010000000
VERSION     DASTMT010000634.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 1599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1599
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5792084"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1100
                     /locus_tag="VFR00_14670"
     CDS             <1..1100
                     /locus_tag="VFR00_14670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007006737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HEU0160563.1"
                     /translation="VGLVPGGSSGGSSAAVAADLCLGATATDTGGSIRQPAAITGTVG
                     IKPTYGRCSRWGIVAFASSLDQAGPIAKSVRDAAVLLRHMASVDGKDSTSVDIAVPDY
                     EAAVGRSIKGLKVGVPREYRMDGMPKEIEQLWQQGIDWLRDGGASVEEVSLPHTKYAL
                     PAYYIVAPAECSSNLARYDSVRYGLREPGQDLIDTYETTRAAGFGAEVRRRVLIGTYV
                     LSTGYYDAYYLKAQKVRTLIKRDFESVFERVDVLLTPTTPGPAFGIGEVSGDPVAMYL
                     NDIFTVTVNMAGLPGISVPAGLSSEATPLGLQLIGKPFDEATLFRVGQVIEDAAGRLR
                     APERWWDARLSSGNPSGAAAVGAAGKLRGRT"
     gene            1097..1420
                     /locus_tag="VFR00_14675"
     CDS             1097..1420
                     /locus_tag="VFR00_14675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEU0160564.1"
                     /translation="MSVRGPAECKDLAEVRVEIDRIDNALIDRIAERLGYVERAWQLK
                     LELRQEANVPWRNQQVIDKVRARAGEKGVPPDLCEALWRQMIGWFIQYEEEKLRDQLE
                     SKSSG"
BASE COUNT          259 a          511 c          550 g          279 t
ORIGIN      
        1 atgtgggcct ggtgccgggg ggatcctccg gcggctcctc ggcggcggtc gccgccgacc
       61 tctgccttgg cgccaccgcc accgatacgg gcggctccat tcgccagccc gcggcgatta
      121 ccggcacggt cggcatcaag ccgacgtacg ggcgctgctc gcgctggggc atcgttgcct
      181 ttgcctcctc cctcgaccaa gccgggccga ttgccaagtc ggtgcgcgat gcggccgttc
      241 tgttgcggca tatggcgagc gtggatggga aagattcgac ctccgtcgat atcgccgtgc
      301 ccgactatga ggccgccgtc ggccgctcga tcaagggtct caaggtgggt gtgcccagag
      361 aataccgcat ggacggcatg ccgaaggaga tcgagcagct ttggcagcag ggcatcgact
      421 ggctgcggga cggcggggcc agcgtcgagg aggtctcgtt accgcacacc aaatatgccc
      481 tgccggccta ctatatcgtc gctcccgccg agtgctcctc caacctggcg cgctacgaca
      541 gcgtgcgcta tggcctgcgc gagccgggtc aggatttgat cgacacctac gagacgacgc
      601 gcgcggcggg cttcggcgcc gaggtgcgcc ggcgcgtgct gatcggcacg tatgtgctct
      661 ccaccggtta ttacgatgcc tattacctca aggcgcagaa ggtgcgcacg ctgatcaagc
      721 gcgatttcga gagcgtgttc gagcgcgtcg atgtgctgct gacgccgacc acgcccggac
      781 cggccttcgg catcggtgag gtgtcgggcg atccggtggc gatgtacctc aacgacattt
      841 tcacggtaac cgtcaacatg gcgggactgc ccggtatttc cgtgccggcc gggctctcct
      901 cggaggcgac gccgttgggg ctgcaactga tcggcaaacc gttcgacgag gcgacgttgt
      961 tccgcgtcgg ccaggtgatc gaggacgcgg ccgggcgctt gcgggcaccc gagcgctggt
     1021 gggatgcccg cctgagcagt ggcaacccgt ctggggctgc cgcggtcggc gcggctggca
     1081 agctgagagg caggacatga gcgtgcgagg ccccgcggag tgcaaggatt tggccgaggt
     1141 cagggtcgaa atcgaccgca tcgacaacgc tctgatcgat cgcatcgccg agcgcctcgg
     1201 ctacgtcgag cgcgcctggc agctcaaact tgagttgcgc caggaagcca acgtgccctg
     1261 gcgcaatcag caggtgatcg acaaggtccg ggcccgcgcc ggcgaaaagg gcgtgccgcc
     1321 ggacctgtgc gaagccctct ggcgccagat gatcggctgg ttcatccagt acgaggagga
     1381 aaaattgcgt gaccagttgg aaagcaagtc ctccggctga aacgttgccg aaacgccgaa
     1441 gggtggcgca tgccgccgat tgaacccgtc aatctcgccg ccgtggtttt gactgccctg
     1501 ttaaatcccg ccgtggtgct cgtcgccgtc tggctcgggc gctacgccga tcaatggcag
     1561 aagctgcccg tggccgcttt tgccgccgcc ctcgtcggc
//
LOCUS       DASTMT010000635         3217 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_3874301, whole genome shotgun sequence.
ACCESSION   DASTMT010000635 DASTMT010000000
VERSION     DASTMT010000635.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3217)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3217)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3217
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3874301"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1263)
                     /locus_tag="VFR00_14680"
     CDS             complement(<1..1263)
                     /locus_tag="VFR00_14680"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0160565.1"
                     /translation="MSRPPRPILGLLVVGLGTLLAPLDTAVNIAFPSITRAFALELED
                     IRWVVIAYVLTYASLMLAFGRLGDLVGYRPIFQVGLAVSALGLFTCSIAPNYAALLLG
                     RALQGVGAALVLSCGPALVTSLLPESERARALAFYAGVIAVGGALGPIVGGALVARWD
                     WSGVFAFRIPVALLALALSPRIASARPQTSMREFDVLGALLLVAWLSALLLAVACAPG
                     PFATVVPYGLSLLGAAAFAVFCRHELRSPHPLLRLSLFGHGSFALLNTINVLASFAAF
                     SVLLLVPYYLVRIAGLDALSGGAVLAVSALGTVLGASLAGRLSRHLPIGALALSGLVL
                     NIAGLWGISRWGLATPLAQIAAALLAQGIGMGLFQVGYSDYVTGALPAQDRGVAGSLT
                     MLTRTIGVVLAATGLSAAFAHFEAGAARA"
     gene            complement(1362..2585)
                     /locus_tag="VFR00_14685"
     CDS             complement(1362..2585)
                     /locus_tag="VFR00_14685"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0160566.1"
                     /translation="MMAERRILFWMCVLVAVNQLGFGAMVPSLPLYAQSFGVPASAIG
                     MAVAAYGLARFLSAVPAGQLADRLGRRPTLAIGGLISALGNLWCAWASAYPELVAARF
                     VAGAGAGLILTTGQVVLADISTPAQRGRSIAVYQGTFLFAVGIGPFPGGLLAEHLGLA
                     APFVAYGIASLLAAGVAWFAVSETRDLWRVNAPTAVVPRLPLGQQLRLLLGKVGFVLV
                     SLISLMNAVVRTGGLFVIVPMLAAALLGLSVGAIGFALMLGSICGVLAAYPAGWFADR
                     FGRKVVIVPATVMTGTSMLLFCVAPNYAWFIAACVVWGVASSVGGAAPATYAADTAPP
                     GMNATAMSAFRMTADAGYVIGPLALGLLADLYGPVVSLVGAAVLLVLVAAAFALLAPE
                     THPRRERVPGRQGDG"
     gene            2956..>3217
                     /locus_tag="VFR00_14690"
     CDS             2956..>3217
                     /locus_tag="VFR00_14690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007820871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HEU0160567.1"
                     /translation="MYQHTVDFIIVGAGSAGAALAHRLSESGRYQVLLLEAGGETHPL
                     SRVPISFARFINRPGVNWLYASEPEANTGGRAIPVPRGRMLGG"
BASE COUNT          648 a         1063 c         1140 g          366 t
ORIGIN      
        1 ggcacgggcc gcgcctgcct cgaaatgcgc aaacgctgcc gagaggccgg tggccgcaag
       61 caccacgccg attgtccgcg tcaacatggt caggctgccg gcgacgccgc ggtcttgcgc
      121 gggcaaggca cctgtcacgt agtcgctgta gccgacctgg aacagcccca tgccgatacc
      181 ctgcgccaac agcgctgcgg cgatctgcgc aaggggtgtc gccagccccc agcgtgagat
      241 gccccagagg ccggcaatgt tgagcacaag ccccgagagc gccaaggcgc caatgggcag
      301 gtgccggctc agccggcccg cgagcgacgc gccgagcacc gtgcccaggg cgctaacggc
      361 aaggacggcg ccgccgctta gggcatcgag gcctgcaatg cgcacgaggt aatagggaac
      421 gagcagcagg acgctgaagg cggcaaagct cgcgaggaca ttgatggtgt tgaggagggc
      481 aaaactgccg tgaccgaata gggagaggcg caacaacggg tgcggcgagc gcagctcgtg
      541 gcggcagaag acggcgaatg ccgcggcacc gaggagcgag aggccatagg gaacaacggt
      601 cgcgaacggc cccggggcgc aggccacggc gagcaacagc gcgctcaacc aggcgacgag
      661 gaggagcgca ccgaggacat cgaattcgcg catcgaggtc tgcggccgag cgctcgcgat
      721 gcggggcgat aaggcgagcg ccaacaaggc aacggggatg cggaaggcga acacgccgga
      781 ccaatcccag cgagcgacga gagcaccccc gacgatgggg ccgagcgcgc cccccacggc
      841 gatcaccccc gcatagaagg caagcgcccg ggcgcgctcg ctttcgggca gcagcgacgt
      901 caccagggcc gggccacaac tgaggaccaa ggccgcaccg acgccttgca gggcacggcc
      961 cagcagcaga gccgcgtagt taggtgcgat cgagcaggtg aaaagaccga gcgcgctaac
     1021 ggcaaggccg acttggaaga tcggccggta gcccacgaga tcgccaagcc tgccgaaggc
     1081 cagcatgaga cttgcatagg tgaggacgta ggcgatgacg acccagcgaa tatcctcgag
     1141 ctcgagggcg aaggcgcgcg taatgctggg aaaggcaata ttgaccgcgg tatcgagcgg
     1201 cgccaacagg gtgccaaggc cgacgacgag caacccgaga attggacgtg gggggcgtga
     1261 catgggcgag cgatcttggc atccgggggc gggcttacct cgatactcca attcgatccc
     1321 ctgccgcagc cttctgtggc gcgatttgcc cccgggctgg ctcagccgtc tccctgccgg
     1381 cccgggaccc gttcccggcg cgggtgggtt tccggcgcaa gcagtgcgaa tgcggcagcc
     1441 accagcacca agaggacggc ggcccccacc agggagacca ccggaccgta gagatccgct
     1501 agcagcccca gggcgagcgg cccgatcaca tagccggcat cggcggtcat gcggaaggca
     1561 ctcatggccg tggcgttcat cccggggggc gccgtatccg cagcataggt ggccggcgcc
     1621 gcgccgccaa cggagcttgc cacgccccag accacgcagg cggcgatgaa ccaggcgtaa
     1681 ttgggggcca cgcaaaacaa cagcatcgag gtgccggtca tcaccgttgc cggcacgatg
     1741 accaccttgc ggccgaaccg atcggcgaac cagccggccg ggtaggcggc aagaacgccg
     1801 cagatactgc cgagcatgag cgcaaaaccg atggcgccga ccgagaggcc gagcagggct
     1861 gcggccagca tcggcacgat gacgaacaac ccacccgtgc ggaccacggc gttcatcagg
     1921 gagatcagac tgaccaagac gaacccgact ttgcccagca gcagacgaag ctgctgaccg
     1981 aggggcaagc gaggcacgac cgcggtcggg gcgttgacgc gccagaggtc gcgcgtctcg
     2041 ctgacggcaa accaggcgac gcccgccgcc aacagactgg cgatgccgta ggcgacgaac
     2101 ggggccgcca atcccaggtg ctcggcgagc aggccgccgg ggaacggacc aattccaacg
     2161 gcgaacagaa aggtgccctg atagacggcg atgctgcggc cgcgctgggc gggtgtgctg
     2221 atgtcggcca ggaccacctg accggtcgtg aggatgagac cggcacccgc tccggcgacg
     2281 aagcgggcgg ctacaagctc cggataggcg cttgcccagg cgcaccagag attgccgagc
     2341 gctgaaatga gcccgccgat agccagcgtc ggccgccggc ccagccgatc cgccaactgc
     2401 ccggcgggaa ccgccgagag gaagcgggcg agcccataag cggccacagc catgccgatg
     2461 gcagatgcgg gtaccccgaa ggattgggcg tagagcggca gcgacggcac catggcgccg
     2521 aaaccgagct ggttcacggc gacaagcaca cacatccaga agagaatgcg gcgttcagcc
     2581 atcatgggag caggacgggg cgatgtcgcc gctgatgccg gccctccgca gacgcgccac
     2641 ctcaatcaac cttcgcgcta tgctgtaggc caaggcgcgg gcgccgacaa tggccggcag
     2701 tgccgcaacg gctgcgagcc gacccctccc ttggccgcgc gggggccgcc cggcgttgac
     2761 gttgcgctgg cgccgagggc cccgcgaccc ggcgcggtca tccggcccgg acttgggggt
     2821 accaccccgg cggctcgatc cggcaccgct tggttgggct tttgccgccg gagccgcgac
     2881 gcgagcgggt ggtggaagcc ataaatcctg ggctagtgtg ccgctgctag gcaacagcag
     2941 cagccgggga agcgcatgta tcagcatacc gttgacttta ttatcgtcgg cgccggctcg
     3001 gcgggcgccg cgctcgcgca ccggctgagc gagagcggac gctaccaagt cctgctgctc
     3061 gaggccggcg gcgaaacaca ccccttgtcg cgcgtcccga tcagcttcgc ccgcttcatc
     3121 aatcgacccg gggtcaattg gctctatgcc tccgaaccgg aggccaacac gggcggacgg
     3181 gcgatcccgg taccgcgcgg gcgcatgctg ggagggt
//
LOCUS       DASTMT010000636         2364 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_4543847, whole genome shotgun sequence.
ACCESSION   DASTMT010000636 DASTMT010000000
VERSION     DASTMT010000636.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2364)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2364)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2364
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4543847"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            201..704
                     /locus_tag="VFR00_14695"
     CDS             201..704
                     /locus_tag="VFR00_14695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0160568.1"
                     /translation="MAAYIAWLRGGTRAIGVHNFRLLLPNARLTFVQIGIGMLDLVVG
                     CLATYLLLPATPSTDWLALAVAYVLAALFGFLCHAPASLGAFEVAMLVLLPQYQTEAL
                     LGALLILHFLYLVLPLALALLALLLHEARLGRAGAVKRDAAPAARREADPKSSGTAAA
                     QVVTSLV"
     gene            complement(763..1374)
                     /locus_tag="VFR00_14700"
     CDS             complement(763..1374)
                     /locus_tag="VFR00_14700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014492381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEU0160569.1"
                     /translation="MAASKRPRGRPRSYDPELALRQALDAFWTQGYSATSLDDLGAAM
                     GMNRPSLYAAFGDKQALYLKALESYWDQALVAMRALLSRDEPLAQALMRIYDAALSLY
                     FPPSGAPRGCFGISTAAVEAVTAPAIRAAFEGGLRRIDKEFEHRFARAVELGELPAAA
                     NPRTLAEIASALLHTLAIRSRYGASRKELRRLAERAVMHMLQA"
     gene            1484..2119
                     /locus_tag="VFR00_14705"
     CDS             1484..2119
                     /locus_tag="VFR00_14705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007614301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HEU0160570.1"
                     /translation="MDLYFAPLACSMASRIALYEAGAAANFIEVDPKSKRTLDGVDYR
                     TVNPLGLVPALRTEAGTVLLENAAILQFIADRYPAAALAPRDGLDRAQLQQWLSFIGT
                     ELHKALFVPLFDKALADSTKAKVLETGGQRLAYLERYLTGREFLLDAFSVGDAYLFTV
                     LNWNIATPVKLSLWPAIENYFARLKERPAIARALKEEFALYAEEQKRHKAA"
BASE COUNT          402 a          724 c          799 g          439 t
ORIGIN      
        1 cgttcgacgt ggcggggact ggaaacgtgg cacggtcgag atgctgtgac acctcgcaac
       61 cgaaagggcg aggcaacggt gaacacaaac atcgacctaa accggcgcgc cagtcttcga
      121 ccctacctag gagcgcccgg aggtgcaatt cctccgggcg actcgccaac caggcgctgg
      181 gcatgggagc gcttggcagc gtggccgcat acatcgcctg gctgaggggc ggaacgcgcg
      241 ccatcggggt tcacaatttc cgcctgctcc tgccgaacgc gcgattgacg tttgtccaga
      301 ttggcatcgg catgctcgat ctcgtggtcg gttgtctggc aacctacctg ttgttgccgg
      361 ccacgccgtc gaccgattgg ctcgcccttg cggtagccta cgtgctggcc gcgctgtttg
      421 gcttcttgtg ccatgccccc gccagcttgg gggcgttcga ggtggcgatg ctcgtattgc
      481 taccgcagta ccaaacggaa gcgctgttgg gagcgctgct gatcttgcac tttttatatt
      541 tggtgttgcc cctggccctg gcgctgctgg cgctgctgct gcacgaggca agattgggca
      601 gggcgggtgc ggtgaaaagg gacgccgccc cggcggctcg gcgcgaagcc gaccccaagt
      661 ccagcggcac cgccgcagcc caggtcgtga ccagcttggt ctgaaacgcc gctcgatggc
      721 cggcatggcc gcgtcgcaga ccgcgcccct cgtcctgcca aactatgcct gcaacatgtg
      781 catgaccgcg cgctcggcca ggcggcgcag ctctttgcgg gaggcgccgt agcgtgatcg
      841 aatggccaag gtgtgcagca gggcggaggc gatctcggcg agcgtgcgag ggttggccgc
      901 agccggcagc tcgccgagct cgacggctcg cgcgaagcga tgctcaaact ccttgtcgat
      961 cctgcgcaag ccgccttcga aggcggcgcg gatggcaggt gcggtgacgg cttcgacggc
     1021 cgcggtgctg atgccgaaac agccgcgcgg cgcgccgctc ggcggaaaat aaagggacag
     1081 cgcggcatcg tagatgcgca tcagcgcctg cgcgagcggc tcgtcgcgcg acaacagggc
     1141 ccgcattgcc accagcgcct ggtcccaata gctctccagc gccttgagat agagggcctg
     1201 cttgtcgccg aaggccgcat agaggctggg tcgattcatg cccatggcgg cgcccaggtc
     1261 gtcgagggag gtcgccgagt agccctgcgt ccagaacgcg tcgagcgcct ggcgcaaggc
     1321 cagctcggga tcgtagctgc gtgggcggcc acggggtcgc ttgctcgcgg ccacgctggc
     1381 cgcctttaca tttttttgta ccatttcgca attaattctt gactgctgcg tctgcccgaa
     1441 tttaaatgcg gatcagtaca aaaacaagtc attggagacc ggcatggacc tttactttgc
     1501 cccgttggcg tgctcgatgg cctcacgcat cgccctctat gaggcgggcg cggccgcaaa
     1561 tttcatcgag gttgatccga aatcgaagcg cacgctcgac ggcgttgact ataggaccgt
     1621 caatccgctg ggcctggtgc cggcgctgcg caccgaggcc ggcacggtac tgctggaaaa
     1681 tgcggctatt ctgcagttca tcgccgaccg gtaccctgcg gcggcgttgg caccgcgcga
     1741 tgggctggat cgcgcccaat tgcagcaatg gttgtcattt atcggcacgg agctgcacaa
     1801 ggcgctgttc gtacctttgt tcgacaaggc actggccgac agcaccaagg ccaaggtgct
     1861 ggagacgggt ggtcagcgct tggcctatct ggagcgttac ctgacggggc gcgaattcct
     1921 gctcgatgcc ttcagtgtgg gagatgccta cctctttacg gtgctgaact ggaacatcgc
     1981 cacccccgtg aagttgagct tgtggccagc gattgaaaat tactttgctc gcctcaagga
     2041 gcggccggcg atcgcgcgcg cccttaaaga agagtttgcg ctctacgccg aggagcagaa
     2101 gcgacacaag gccgcctgag agggcagcct gtgagggcag cctgaggtga gagttggggg
     2161 cggcgcgcgg gccaatcctc gcccgcttcc gccaagcgcg attgcaggcc ggtgttgtcc
     2221 gaggctgggg ccgggcgcgg ttgcccgcga gagggcgcag atctatggtg tcgggctcgc
     2281 ccacttgggt cgcaaaacgc caaagcccgg ggatggcctg atggaaggtg gctgcacctg
     2341 tcggcaactt cgctatcgct tgca
//
LOCUS       DASTMT010000637         1530 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_5527310, whole genome shotgun sequence.
ACCESSION   DASTMT010000637 DASTMT010000000
VERSION     DASTMT010000637.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 1530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1530)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1530
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5527310"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            274..792
                     /locus_tag="VFR00_14710"
     CDS             274..792
                     /locus_tag="VFR00_14710"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HEU0160571.1"
                     /translation="MFERNRVDNAPEVSAVPVELTLLDGTLVKGKLLVPVLKTVVDTL
                     NGGTAFIEFEPYGGERRYIAKAQLAAVKPVGVPRVPALQSRLRDNGEFDPYAVLGLAH
                     GAAREQVREAYVALAKAYHPDRYANVALPPEVGDYLAAMARRINAAHAALEAPEKRRA
                     VRQEPIFTSPGR"
     gene            complement(801..1460)
                     /locus_tag="VFR00_14715"
     CDS             complement(801..1460)
                     /locus_tag="VFR00_14715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0160572.1"
                     /translation="MSLLIATGGVAYITNIYQKRSSFVDALKEEWRDIIAAKSALFSY
                     TQLERPSLEMYLATFCKLSETIDNMRSVYQNVGETGDLVGLYPYAPLHDMRRALQTLD
                     PRKNPQAPDAESRALVRDAILQSFYALRETFLEELDLEEPDHPLLISGGRRAKVSGST
                     SGARRRQERQRARQDRVSASNPRIDLLLAELMQKEEQTAKPWRQPAASAPAMPANGRA
                     S"
BASE COUNT          270 a          492 c          492 g          276 t
ORIGIN      
        1 cgggccgccc ggcgcggcgc acgagccagg ccgaggccag ccgccctggc cggtcacggc
       61 cgatccgacg gccgcagcaa agccgagaac gggggctatg ccctctttaa gggtcaaaac
      121 ggggccagga cccgccacca gcatgcctcg ccacccccgc tgcaaggctg gggccaaggt
      181 cagccgcgaa actccggctg ggcccctttc ttgctccgtc gttcagttgg acgccgccgg
      241 cgttgcaaaa cgacacaggc aagagagggc ctcatgtttg agcgcaatag ggtcgacaat
      301 gcacccgagg tgagcgccgt gccggtcgag ctcaccttgc tcgacggcac gctcgtcaaa
      361 ggcaagctcc tcgtgcccgt cctcaagacc gtggtcgata ccctgaacgg tggcaccgcc
      421 ttcatcgagt ttgaacccta cggcggtgaa aggcgctaca tcgccaaggc gcagcttgcg
      481 gccgtcaagc cggtgggcgt tcccagggtg ccggccttgc aatcccgcct ccgcgacaac
      541 ggcgaattcg acccttatgc cgtgctcggc cttgcgcacg gagctgcccg agagcaggtg
      601 cgggaggcct atgtggcgct agccaaggcc tatcatcccg atcgctacgc caatgtggca
      661 ctgccgccgg aagtcggcga ctacttggcc gccatggcgc ggcgcatcaa tgctgcccac
      721 gccgccctcg aggcaccgga aaagcggcgg gccgtgcgcc aggagccaat cttcacgtca
      781 cccggccgct aagcgccgcc tcaagacgcg cggccgtttg ccggcattgc cggcgcgctt
      841 gcggccggtt gtcgccaggg tttggccgtc tgctcttctt tctgcatgag ttcggccagc
      901 agcagatcga tgcgcgggtt cgacgcgctg acacgatcct ggcgcgcgcg ctgccgttct
      961 tgccggcggc gcgcgccgct ggtcgagccc gacactttgg ctctgcggcc gcccgaaatg
     1021 agcagtgggt gatctggttc ctcgaggtcg agctcctcca ggaaggtttc acgcagggca
     1081 tagaacgatt gcaggatcgc atcgcgcacc aaggcgcgcg actcggcgtc gggcgcttgc
     1141 ggatttttgc gcggatcgag ggtctgtagc gcgcgccgca tgtcgtgcag cggtgcgtaa
     1201 ggataaagac ccacgagatc gccggtctcg ccgacgttct gatagaccga gcgcatgtta
     1261 tcgatcgtct cggacagctt acagaaagtg gcaagataca tttcgaggct cggcctctcg
     1321 agctgggtat aggagaacag cgccgatttg gcagcaatga tatcgcgcca ttcctccttc
     1381 agggcatcga cgaagctcga tcgtttttgg tagatgttgg tgatataggc aactccgccg
     1441 gtggcgatca gcaacgacat gtcacgcagg aattcttagg cgtcttttag tatcgcctcg
     1501 agaccggatc ctcttaatgg cgcgatgtga
//
LOCUS       DASTMT010000638         4106 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_3878965, whole genome shotgun sequence.
ACCESSION   DASTMT010000638 DASTMT010000000
VERSION     DASTMT010000638.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4106)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4106
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3878965"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1033
                     /locus_tag="VFR00_14720"
     CDS             <1..1033
                     /locus_tag="VFR00_14720"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEU0160573.1"
                     /translation="GHAAPNLQHWLAAIETRRADVGRALGTHLPARPPTFCTGCPERP
                     VFSAMKLLKQELGPVHVAADIGCHAFATFAPFSQGNSILGYGMSLASAAAVSGTQRAR
                     PISVMGDGGFWHNGLLTGVAGAVFNNDDSVLVVMNNGYSSATGQQDILSSSRAQDGRG
                     KGLDIEATLKSMGVNWIKRVHTYRVASVLEALRQAMRSTQRGLKVIIADGECQLARQR
                     RLRPQLARLLGAGKRVVRTRFWVDPEICTGDHSCIRLSGCPSLTLKANEDALRADPVA
                     HVNNDCVGCGLCGEVAHAAQLCPSFAQIDVIQNPNWWDRLRARLVARLIGRTAGPGSR
                     RRAAVVPAE"
     gene            1039..2562
                     /locus_tag="VFR00_14725"
     CDS             1039..2562
                     /locus_tag="VFR00_14725"
                     /inference="COORDINATES: protein motif:HMM:NF006179.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta
                     family protein"
                     /protein_id="HEU0160574.1"
                     /translation="MPEAAPIKLLLAALGGEGGHVLASWLHDAAIASGHYVQGTFIPG
                     VAQRTGATTYYLEIVPGAASRHAAADQRPVLALNAAPGEVDVMVASELLEAARAIEAG
                     FVSPERTTLITSSVRVFTVDEKAAMTDGRLDAHALRDVARTFSRRLIIADYTGAAAAA
                     NSVLNAVLLGALAGAGALPIALEEYRRAIRRQGKSVDDNLQGFAAGLAAQGEDSATRW
                     VHAPVAPALAAGVSAAALGEGALLAFPVEARDVLKSAVARLVDYQDLAYARLYLARLS
                     GFLGRCGSDGDFIRELARHLALRMSVEDVIRVAQLKLRRTRLARLGQEARARAGDILE
                     VTEYLKPGPEEVLGLLPPRVGRWLLARVRRERGWTLKVRSTRLSGFLRLRALAALRRW
                     RPKTLKFAEEEAWVRRWLDLVERAYALDPAAAREVVATAALVKGYAETYKRGLANWNR
                     IATAVIEPALAGRLAGVAFADAVLQARLAASKDPEGETLRRTIDAIVAGATGKLAAQ"
     gene            complement(2603..>4106)
                     /locus_tag="VFR00_14730"
     CDS             complement(2603..>4106)
                     /locus_tag="VFR00_14730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0160575.1"
                     /translation="LIALLRPGLGQELAELVTRLLWPTALFAALCAVVGREAYRRAGP
                     WAVAAVAVFAATSIGATGQFRPGRIDHHNVQILCAVASLLLLVRSCNDHRLGWSAGAL
                     IGVGLAIGYEAIALVGPALALAALIAVMQPARRLRLLPVGVAATVSLLAAGVLTTPPA
                     TWLTSRCDALSVNLIVVAASGTCGLWLAERFAQRWEARLASLAAAALLGASAFAALEP
                     ACLAGPFGEVDPALKPIWLDHVLETKSLLWFAGREPAFALAQFGFLLAGAAAQLLAWR
                     RRRDAESTLATGIVLLAVGLACWQMKLVAYASWLALLPLACLCGRLAGTARLASGWVR
                     AAAVVLLSQATLEAVFSLAAAAVPSPPRQGSAQLVAADPRGPCFQSASLSALAALRPG
                     LVAADLDLGPYVVALTPHRVVAAPYHRIAKGILANHAILDAELDGAEAAMRGLGVDYI
                     ALCNRTGTLPGGTARSSLREALLGNGALGFLEPVAGTSKAPIRVWRRSPP"
BASE COUNT          651 a         1374 c         1456 g          625 t
ORIGIN      
        1 tggccacgcc gcgcccaacc tgcagcattg gctggccgcg atcgaaacgc ggcgggccga
       61 cgtcggccgc gcgctgggca ctcacctgcc cgcgcgtcct ccgacgttct gtaccggctg
      121 tccggagcgc ccggttttct cggccatgaa gcttttaaag caggaactgg ggcccgtgca
      181 tgtggctgcc gatatcggct gccatgcctt tgcaaccttt gcccccttca gccagggcaa
      241 ctcgattttg ggctacggca tgtcgctggc ctccgccgct gccgtttcgg gtacgcaacg
      301 cgcgcgcccg atcagtgtca tgggtgatgg tggtttctgg cacaacgggc tgctgaccgg
      361 cgttgccggc gcggtgttca acaacgacga cagtgtgctg gtggtaatga acaatggcta
      421 ttccagcgcc accggtcagc aggacattct gtcgagcagt cgagcgcagg acggtcgcgg
      481 caaaggcctc gacatcgagg ccacgctcaa gagcatgggc gtgaactgga tcaagcgcgt
      541 gcacacctat cgcgtcgcgt cggtgctcga ggcactgcgc caggccatgc gcagcacgca
      601 gaggggtctc aaggtcatca ttgccgacgg cgagtgccag cttgcccggc aacggcggct
      661 gcgaccccag ttggcgcggc tgctcggcgc gggcaagcgg gtggtgcgca cgcgtttttg
      721 ggtcgatccg gagatatgca cgggggatca ttcctgcatc cgcctctccg gctgcccctc
      781 gctgacgctc aaagccaacg aggatgccct gcgtgcggat cccgtggcgc acgtcaacaa
      841 cgactgcgtt ggctgtggct tgtgcggtga ggtcgcgcat gcggcgcagc tatgtccgtc
      901 cttcgcgcaa atcgacgtga tccagaaccc caactggtgg gaccggctgc gcgcgcgcct
      961 cgttgcccgc ctgatcgggc gcacagccgg cccgggaagc cggcggcgcg ccgccgtcgt
     1021 gccggccgag tgagcgccat gccggaggct gcgccgatca aactgctgct cgcggccctc
     1081 ggtggtgagg gagggcatgt cttggcctcc tggctgcacg atgccgccat tgccagtggc
     1141 cattacgtgc agggaacctt tattccgggg gttgcgcagc gcaccggcgc aaccacctac
     1201 taccttgaaa tcgtgccggg ggcagcgtcg cggcatgcgg ccgccgacca gcgccccgtg
     1261 ctggcgttga acgcggcgcc gggcgaagtc gatgtcatgg tggcaagcga gctgttggag
     1321 gcggcgcgcg caattgaggc cggcttcgtt agccccgaac ggacaactct catcacctcg
     1381 tccgtgcgtg tcttcacggt cgacgagaag gcggccatga cggacggccg cctcgacgcg
     1441 cacgccttgc gcgacgtcgc ccgcaccttt tcccgccgtc tcatcattgc cgattacacg
     1501 ggcgcggcgg ccgcggccaa cagcgtgctc aacgccgtgc tgttgggcgc gcttgccggc
     1561 gccggtgcgc tgccgattgc gctcgaggaa tatcgccgcg cgatccgccg acaaggcaag
     1621 tccgtcgacg acaacctgca gggatttgct gccggtctgg cggcccaggg ggaggacagc
     1681 gccacacgct gggtgcatgc tccggttgcc ccggcgctgg ctgcaggcgt cagtgcggcg
     1741 gcgctgggcg agggcgcgct gctggccttt ccggtcgagg cgcgcgacgt actgaagtcg
     1801 gcggtggcgc ggctggtcga ctatcaggat ctagcctatg cccggctcta tctcgcgagg
     1861 ctcagcggct ttctcggccg ctgcggcagt gacggggact tcatccgcga gctcgcgcgc
     1921 catcttgcct tgcgcatgag cgtcgaagat gtgatccgcg tcgcccaact caaattgcgc
     1981 cgcacgcggc tcgcgcgcct cgggcaggag gcacgcgcgc gggcgggcga tatcctcgag
     2041 gtgaccgagt acctgaagcc ggggccggaa gaagtcctgg gcctgctgcc gccccgtgtc
     2101 gggcgatggt tgctcgctcg cgtgcgccgc gagcgcggct ggacgctgaa agtgcgcagc
     2161 acgcggttgt cgggcttctt gcgcttgcgc gctctggccg cgctccgacg ttggcgcccg
     2221 aagacgctca agtttgccga agaggaggcc tgggtgcgcc gttggctgga cctggtcgag
     2281 cgtgcctacg cgctcgatcc ggctgccgcc cgcgaagtgg tggcgaccgc cgcgttggtc
     2341 aagggttatg ccgaaaccta taagcgcggg ctcgcgaatt ggaacaggat tgcaaccgcg
     2401 gttatcgaac ccgcactcgc cggtcggctg gcgggcgttg ctttcgctga cgcggtgctg
     2461 caggcccgcc tggcggcgag caaagatccc gagggcgaga ctttgcggcg caccattgat
     2521 gccatcgttg ccggtgcgac gggcaaactc gcagcgcagt gaagcgcgca gcccgaccta
     2581 tcgtcgccag ccgtgtcggc gctcaaggtg ggctgcggcg ccagacgcgg atcggcgctt
     2641 tcgacgtccc cgccacgggc tcgaggaacc cgagcgcgcc gttgccgagc aaggcttcgc
     2701 gcagcgagga tctggcggtc cccccgggga gggtgccggt gcggttgcac aaggcgatgt
     2761 agtcgacgcc aagcccgcgc atggccgcct cggcgccgtc gagttcggcg tcgaggatgg
     2821 cgtgattggc caatatgcct ttggcgatgc gatggtaggg cgccgccacc acccgatgcg
     2881 gggtcaaggc aacgacatag ggtccaaggt cgagatcggc ggccaccagc ccgggccgca
     2941 acgcggccag cgcgctgaga cttgccgact ggaagcaggg gccgcgcgga tcggcggcga
     3001 ccagctgggc gctgccttgg cgcggcgggc tcgggaccgc ggccgcggcg aggctaaaga
     3061 cggcctccag cgtggcctgg ctcaagagca ccacggcggc ggcgcggacc cagccacttg
     3121 caaggcgggc cgtgccagca aggcgcccgc acaggcacgc caacggcagc aacgccagcc
     3181 agctcgcgta ggcgacgagc ttcatttgcc aacacgcaag gcccacggcc aacagcacga
     3241 tgcccgtcgc caatgtgctc tccgcgtcgc ggcgacgccg ccacgccagc agctgggccg
     3301 ccgccccggc aagcagaaag ccaaattgcg cgagcgcgaa ggcgggctcg cgccccgcaa
     3361 accataggag actttttgtt tccaggacgt ggtccagcca gatgggtttg agggccggat
     3421 cgacctcacc gaagggaccg gcgagacagg cgggctcgag cgcagcgaag gcgctggcgc
     3481 ccaggagcgc cgcggcggcg aggctggcaa gccgcgcttc ccatcgctgc gcgaagcgct
     3541 cagcgagcca caatccgcag gtgccgctcg ccgccacgac gatgagattg acagacaggg
     3601 cgtcgcatcg gctcgtcaac caagtggccg gtggcgtcgt caggacgccg gcggcaagga
     3661 ggctcaccgt ggcggccacg cccaccggca gcagcctgag ccggcgtgcc ggttgcataa
     3721 cggcgatcag cgccgccagc gccagggccg gtccgaccag ggcgatggcc tcgtagccga
     3781 tcgcgaggcc gacgccgatc agggctccgg cgctccaacc gaggcgatgg tcgttgcagc
     3841 tgcgcaccag caaaagtagg cttgcgacgg cgcaaaggat ctgcacgttg tgatggtcga
     3901 tgcggcccgg cctaaattgg ccggtggcac cgatcgaggt ggcggcaaac acggccaccg
     3961 cggcgacggc ccaaggtccg gcgcgccggt aggcctcgcg tccaacgacc gcgcacaagg
     4021 cggcaaacaa ggcggttggc catagcaacc gcgtcacgag ttcagcgagc tcctggccca
     4081 gccctggccg caacagcgca atcagc
//
LOCUS       DASTMT010000639         2340 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_5205278, whole genome shotgun sequence.
ACCESSION   DASTMT010000639 DASTMT010000000
VERSION     DASTMT010000639.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2340)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2340
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5205278"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..940
                     /locus_tag="VFR00_14735"
     CDS             <1..940
                     /locus_tag="VFR00_14735"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEU0160576.1"
                     /translation="SSQISVGTGGKQVLYNALTATLNPGDEVVIPAPCWVSYADIVLL
                     GGGKPVFVPCKLEDGYKMQPEAFAAAITARTKWFVFNAPCNPTGAAYSKAELKALTDV
                     LMQPKAKHVWVLTDDMYEHLLYDEAKFYTPAQVEPGLYERTLTLNGLSKTYCMTGWRL
                     GYGAGPEPLIKAMCKLQSQSTSNPSSITQWAAIEALNGPQDFIKRNNQVFRERRDLVV
                     SMLNQAKGLKCPKPEGAFYVYPSCEGLIGKTSGKGTKLATDEDVVLAMLEEEDVACVH
                     GSAFAMSPFFRISYATSTEVLEEACKRIQRFCGNVH"
     gene            complement(1094..1873)
                     /locus_tag="VFR00_14740"
     CDS             complement(1094..1873)
                     /locus_tag="VFR00_14740"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU0160577.1"
                     /translation="MKKPVAVITGGASGIGEATARRFLADGYAVAILDQNDAAGAALA
                     SSLAGSAQFYACDVSDAAGLDAVAGKAHSDLGAADVLVTSAGLIPNCEGIMDMDMAAH
                     DRMWQVNYNGTLHAVRAFARQMIPRQRGAIVTLGSINSRMPLPIPAYNMGKAAIERLT
                     QLLAVELGRHGIRVNSVAPTYVMTPPLRAKVKSGQRDMNKIMAVHALKELPEPKDIAD
                     AIAFLCSAQARAITGVLLPIDAGWLAGVSYRTYAGGVPWQE"
     gene            complement(2018..>2340)
                     /locus_tag="VFR00_14745"
     CDS             complement(2018..>2340)
                     /locus_tag="VFR00_14745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012109946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HEU0160578.1"
                     /translation="LIGIPWGESIMAGSLLGVKTVLNELIAYLQLAGTSAEALSPRSR
                     LILTYALCGFANLGSLGILIGGMVAMVPGRRAEIVSLAARTLVSGTLATLMTAAVVGA
                     LTPA"
BASE COUNT          416 a          761 c          762 g          401 t
ORIGIN      
        1 cagctcgcag atttccgtgg gcaccggcgg caagcaagtg ctctacaatg cgctgaccgc
       61 gacactcaat cctggcgatg aggtcgtgat cccagcgccc tgctgggttt cctacgccga
      121 tatcgtgttg ttgggcggcg gcaagcccgt gttcgtgccc tgcaagctcg aggatggcta
      181 caagatgcag cccgaggcgt ttgcggctgc catcacggcg cgcaccaagt ggttcgtctt
      241 caacgcgccg tgcaatccga cgggagcggc ctattccaag gctgagctca aagccctgac
      301 cgacgtgctg atgcagccca aggccaagca cgtgtgggtc ttgaccgacg acatgtacga
      361 gcacttgctc tacgacgagg ccaagtttta caccccggca caggttgaac ccggtctcta
      421 cgagcgcacg ctcacgctca atggtttgtc gaagacctac tgcatgacgg gctggcggct
      481 cggctacggg gccggccccg aacctctgat caaggcgatg tgcaaactgc agtcgcaatc
      541 gacctccaat ccctcctcga tcacgcagtg ggcggcgatc gaggcgctca acgggccgca
      601 ggacttcatt aagcgcaaca accaggtgtt ccgcgagcgg cgcgacctgg tggtctccat
      661 gctgaaccag gccaaaggcc tgaagtgccc gaagccggaa ggagcctttt acgtgtatcc
      721 ctcttgcgag ggccttattg gcaagacctc gggcaaaggt accaagctcg ccaccgatga
      781 ggatgtggtg ctcgccatgc tcgaggagga ggacgtcgcc tgcgtgcacg gctcggcctt
      841 cgccatgagc ccgttcttcc gtatctccta tgccacctcc accgaggtgc tggaggaagc
      901 ctgcaagcgc atccagcgtt tttgcggcaa cgtgcactga ggcgggttgc gccgctcagg
      961 cttgctcgag cggcgcgccc gataccccgc gaccgctggc gcgagccaaa agcgagcctc
     1021 gagcgggcgt cctcgcgcca ccgcccgggg gcaggggccg gtgaggcgac ccaagccgca
     1081 accaagtcgg cgcttactcc tgccagggca ccccgccggc ataggtgcgg taactgacgc
     1141 cggcaagcca acccgcatcg atgggcaaca ggacgcccgt gatggcgcga gcctgcgccg
     1201 agcacaggaa ggcgatggcg tcggcgatgt ccttcggctc cggcaactcc ttcagggcat
     1261 gcaccgccat gatcttgttc atgtcgcgtt gcccggattt gaccttggcg cgcagcggcg
     1321 gcgtcatgac gtaggtcggg gccacactgt tgacgcgaat gccgtgccgg ccgagctcga
     1381 ccgcgagcag ctgggtgagc cgctcgatgg ccgccttgcc catattgtaa gccgggatcg
     1441 gcagcggcat gcgactgttg atggagccca gtgtgacgat ggcgccgcgc tgtcttggga
     1501 tcatctggcg ggcaaaggca cgcacggcgt gcaaggtgcc gttgtagttg acctgccaca
     1561 tgcggtcgtg cgcggccatg tccatgtcca tgatgccttc gcagttgggg atcaatccgg
     1621 ccgaagtgac gagcacgtcg gccgcaccga gatcgctgtg cgctttcccc gccacggcat
     1681 cgaggccagc ggcgtcgctg acatcacagg cataaaattg agcgctgccg gcgagcgagc
     1741 tcgccagcgc cgcgcccgcg gcgtcgttct gatcgaggat agcgacggcg tagccgtcgg
     1801 cgaggaaccg gcgcgctgtc gcctcgccga tgccgctggc gccgcccgtg atcacggcga
     1861 ccggtttttt catgcttgcg ttctcctttg catggctcat tgctttctca atcgctcatt
     1921 gcctcgggcc tcaagggcgc cgcggcaaac acttttgcca agcgacggcg cgaaggcagc
     1981 gcgcgcattg gtgccgcgag gccacgccag cgtggcacta ggcgggcgtc aaggcgccga
     2041 ctaccgccgc cgtcatcaag gtggcgagcg tgcccgagac cagcgtgcga gcggcgagac
     2101 tgacgatctc ggcccggcgc ccgggcacca tcgccaccat gccgccgatc agaatgccaa
     2161 ggcttcccag gttggcaaag ccgcagagcg cgtaggtcaa gatcaggcgt gagcggggcg
     2221 ataaggcttc ggccgacgtg ccggcgagct ggagataggc gatcagctcg ttgagcaccg
     2281 ttttaacccc gagcaagctg ccagccataa tgctctcgcc ccagggaatg ccgatcagcc
//
LOCUS       DASTMT010000640         3506 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_6190230, whole genome shotgun sequence.
ACCESSION   DASTMT010000640 DASTMT010000000
VERSION     DASTMT010000640.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3506
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6190230"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..435)
                     /locus_tag="VFR00_14750"
     CDS             complement(<1..435)
                     /locus_tag="VFR00_14750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019906601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0160579.1"
                     /translation="MTDNTIGKSNRTAGKGKRPAGSQAGTVGKGALVAGGKSASPASG
                     KSAPPASGHSGTTAAAGMPAFLVDLARNAPAMMWTFTLTTFLSALLLFAIQPMFAKTV
                     LPILGGSPSVWSVSVFFFQAALLAGYLYAHLLTTKVPAHLTGI"
     gene            complement(890..1420)
                     /locus_tag="VFR00_14755"
     CDS             complement(890..1420)
                     /locus_tag="VFR00_14755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0160580.1"
                     /translation="MIFRDIAFVTTEVVAIGRIDVLRMHAAEIYVAPAIGEFFDCRYW
                     HNQGGSFFFGQWIDVERAHAMLATIRSAMERDFVDFFEDRAGAENPKTLAASFSSGMG
                     HRLGERLRRLKARRTANALARAKAAAAELARLLQTALAKPIRRHTGSGSAVAYAAGAE
                     AANRVKLADIGKPGAQ"
     gene            complement(1731..2408)
                     /gene="phaR"
                     /locus_tag="VFR00_14760"
     CDS             complement(1731..2408)
                     /gene="phaR"
                     /locus_tag="VFR00_14760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020083975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate synthesis repressor PhaR"
                     /protein_id="HEU0160581.1"
                     /translation="MRVLNKADPPASKDPDKIEPVVVKKYANRRLYNTDTSTYVTLDD
                     LAAMVKSGRDFVVFDAKSGDDLTHSVLTQIIVEQESRMGGQTLLPIPFLRQLIRFYGD
                     SIERLVPSYLQISLDTLTKEQERFRKTFAGAFTTGAFEAYREQAQKNLAMFEQAMAMF
                     SPFGPAKTAGPRSGDEPAKPEPAKPEAAKPAVPAAAAPRSDEIGELKAQLAAMQAKLE
                     QLSRDRG"
     gene            2682..>3506
                     /locus_tag="VFR00_14765"
     CDS             2682..>3506
                     /locus_tag="VFR00_14765"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HEU0160582.1"
                     /translation="MSNNEATIVIAAAARTLVGAFNGALASLPAHDLGKVAIKAALER
                     AGVEPGEVSEVIMGQILTAGAGQNPARQASIGAGIPVDAPAWGVNQLCGSGLRAVALG
                     AQQIELGSSRIVVAGGQESMSQAPHCAHMREGTKMGDVKFIDTMIRDGLWDAFNGYHM
                     GNTAENVARQWQITREEQDRFAVASQNKAEAARKAGKFKDEITPVTIKTRKGETVVGE
                     DEYIKEGVTLESIAKLKPAFDKEGTVTAANASGINDGAAAVVLMTLEEARRRDITPL"
BASE COUNT          598 a         1138 c         1172 g          598 t
ORIGIN      
        1 gatgccggtc aggtgcgccg gcaccttggt ggtcaggagg tgcgcgtaga ggtagcccgc
       61 cagcagcgcc gcctggaaga agaacaccga caccgaccac accgacggcg agccgccgag
      121 aatgggaagc accgtcttgg caaacatcgg ctggatcgcg aacaggagca gcgcggagag
      181 gaacgtggtg agcgtgaagg tccacatcat ggccggggcg ttcctggcca ggtccaccag
      241 gaacgccggc atgccggcgg ccgcagtcgt gccgctgtgg ccgctcgccg gtggggcgct
      301 cttgccgctc gccggcgacg cgctcttgcc gcccgccacc agtgcgccct tacccactgt
      361 gccggcctgg ctgccggccg gccgcttgcc cttgccggcc gtgcgattac tcttgccgat
      421 ggtattgtcg gtcatcccct tccaaccttc tcaactttcc ctgggtctcg catgggcgcc
      481 cgagcttgca ggggttgccg ccgcacgctc gcttggacga tgctggccaa tcgccggcca
      541 atccctctgc cgatacgacc tttggtcgtt catcctggtc acgatgcttt gttgctagac
      601 gggccccgca ggtttgcaaa gctacggttt ggtaatgcct gcgcagcata gtcggaacac
      661 agccgtcccc ccggcggctc gcacgaccct tggcttctaa gggcaacgcc gcagtgccgt
      721 caagctgagg acagcacgcg cagagggctc gcatctcctt cgagcagccg ctgctggacg
      781 ggaaaggcgt attctttggc ggtttcaagc caagacttcg cctgcgttgg cctcgaccgg
      841 tggcgtaccg acccgggcac gagtgcaatc cgtcaatgcg gctgtcgccc tattgcgcgc
      901 cgggtttgcc gatgtcggca agcttgacgc ggttggcggc ctcggcgccg gccgcgtagg
      961 cgaccgcgct gcccgatccc gtgtgccttc tgattggctt cgccagggca gtctggagca
     1021 gccgcgcaag ttccgcagcg gcggctttcg ctcttgccag cgcgttcgcg gtgcgccggg
     1081 ccttgaggcg gcgcaggcgc tcaccgaggc gatgtcccat gccgctggag aagctcgccg
     1141 ccagcgtttt cggattttcg gcgcctgcgc gatcctcgaa gaaatcgacg aagtcccgct
     1201 ccatggcgga ccggatcgtg gcgagcatgg catgggcccg ctcgacatcg atccattggc
     1261 cgaagaaaaa gctgcccccc tgattgtgcc agtacctgca gtcgaagaac tcgccgatcg
     1321 cgggcgcgac atagatttcc gcggcatgca tgcgcagcac gtcgatcctg ccgatggcga
     1381 cgacttccgt cgtcacgaac gcgatgtcgc gaaagatcat gcgcagtcac ctccccgcaa
     1441 ggcagccgcc gcgtacttgg ggtcacagga ttggggggcc gtgaaatgca cgaccaaaag
     1501 tggcaggatg gtggaagatt gccactggac cgcgagaaaa acacaatttc cgatgaattg
     1561 agcccgtata tcctgcagcc gttatcaatc acataagtgt tcccaatggc gcgcgcaaaa
     1621 acaaagaggc gccgaattgc acgaaaatcg aacaatatcc ctcgagcccg ccgtcttccc
     1681 tggcctgtgg gctcgggtca gcatgcggaa gcgtccggat tgtgatcgga tcagccgcgg
     1741 tcgcgcgaga gttgctcgag cttggcctgc atggcggcga gttgggcctt caattcgccg
     1801 atctcgtcgg acctcggcgc cgcggctgcc ggcacggcgg gcttcgcggc ttcgggcttg
     1861 gccggctcgg gcttggccgg ctcatcgccc gaccgggggc cggccgtctt ggcggggccg
     1921 aacggggaga acatcgccat cgcctgctcg aacatggcca ggttcttctg cgcctgctcc
     1981 cggtaggcct cgaaggcgcc ggtggtgaag gcgccggcaa aggtcttgcg gaaccgctcc
     2041 tgctccttgg tcagcgtgtc gaggctgatc tgcaggtagc tcggcaccag gcgctcgatg
     2101 ctgtccccgt agaagcggat tagctggcgc aggaacggga tcggcagcag cgtctgcccg
     2161 cccatgcggc tttcctgctc gacgatgatc tgggtgagca ccgaatgggt gagatcgtcg
     2221 ccgctcttgg catcgaacac gacgaagtcg cggccggatt tgaccatcgc cgccaggtcg
     2281 tcgagcgtca cataggtgct ggtgtcggtg ttgtagaggc ggcgattggc atacttcttg
     2341 acaaccaccg gctcgatctt gtccggatcc ttgctggcgg gagggtcggc cttgttgagc
     2401 acgcgcattg cgccattcgc cgggctgaaa tgatgcagcg cgcacgttaa cacggccgtg
     2461 cgccgccaga ccagcccatt tctttggcat gccacgccgc cgagatcgga tttgcctttg
     2521 cagcttgcgc tccagcaatg tggttcccgg cagttgccat tgcaggcggc agccggcgtg
     2581 ctatcgtgcc atcgtgcagt gcagcgcagg cgtttttgcg ccagcatcag tgcgtgaact
     2641 cgcgagtgcg cgcttcccat caccgacccc tggagccaac aatgagcaac aacgaagcca
     2701 cgatcgtgat cgccgccgcc gcacgaaccc tggttggtgc cttcaacggg gcgctcgcca
     2761 gcctgcccgc ccatgacctc ggcaaggtgg cgatcaaggc ggcgctggag cgcgccggag
     2821 tggagccggg ggaggtgtcc gaggtcatca tgggccagat cctgaccgcg ggtgccggcc
     2881 agaaccccgc gcggcaggcc tccatcggcg ccggcatccc cgtcgatgcg ccggcctggg
     2941 gcgtcaacca gctgtgcggc tcgggcctgc gcgcggtggc gctgggagcc cagcagatcg
     3001 agctcggctc gtcgcgcatc gtggtggccg gcggccagga aagcatgagc caggcgcccc
     3061 actgcgcgca catgcgcgag ggcaccaaga tgggcgacgt gaagttcatc gatacgatga
     3121 tcagggacgg cctgtgggac gccttcaacg gctaccacat gggcaacacg gccgagaacg
     3181 tagcgcgcca gtggcagatc acgcgcgagg agcaggaccg cttcgcggtg gcctcgcaga
     3241 acaaggccga ggcggccagg aaggccggca agttcaagga cgagatcacg cccgtcacca
     3301 tcaagacgcg caagggcgag accgtggtcg gcgaggacga gtacatcaag gagggcgtca
     3361 ccctcgagtc catcgccaag ctcaagcccg ccttcgacaa ggagggcacc gtcaccgccg
     3421 ccaacgccag cggcatcaac gacggcgccg cggcggtggt gctgatgacc ctggaggagg
     3481 cccgccggcg cgatatcacg ccgctg
//
LOCUS       DASTMT010000641         1782 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_5207184, whole genome shotgun sequence.
ACCESSION   DASTMT010000641 DASTMT010000000
VERSION     DASTMT010000641.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 1782)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1782)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1782
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5207184"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..393
                     /locus_tag="VFR00_14770"
     CDS             <1..393
                     /locus_tag="VFR00_14770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008192695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0160583.1"
                     /translation="VRFIEKRRGLSLIYFGRCFIFLGLLTLPITPLTVIVLSALLGLF
                     WLSTVPLTSSLVATFFGTRWMSMLFGVVFLSHQLGSFAGLWLAGTLYDLTKSYDMMWW
                     ISIALGLFAAAVHWPIQERPVPRLVAAT"
     gene            559..759
                     /locus_tag="VFR00_14775"
     CDS             559..759
                     /locus_tag="VFR00_14775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0160584.1"
                     /translation="MAQQSPTAATPSAERQAPSPSMPRALRISLFAALGALLAGAAYL
                     LVVRGEALIVDLAAVSQRIFCF"
     gene            837..1337
                     /locus_tag="VFR00_14780"
     CDS             837..1337
                     /locus_tag="VFR00_14780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HEU0160585.1"
                     /translation="MNVRQAVAMGGAVAKAGLAIAALLALCAPPALAQDLKTYGKKGS
                     YEDVKFELNDAIIRRGLAIDYTGQINKMLERTGADVGSTKKIYANAEFFMFCSAKLSR
                     DMIEADPANLGYCPYVIFLYEAADKPGEIVVGYRRPSPKGDAASQKALAAIDALLDGI
                     VKDAAK"
     regulatory      1442..1653
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
BASE COUNT          315 a          574 c          561 g          332 t
ORIGIN      
        1 gtccgcttta tcgaaaagcg gcgcggcctc agcctcatct atttcggccg gtgtttcatc
       61 tttcttggcc tgctaaccct gccgattacg ccgctgacgg tcatcgtgct cagcgcgctg
      121 ctcggactgt tctggctgtc gacggtgccg ttaacctcaa gcctggtggc cacctttttc
      181 ggcacgcgtt ggatgtcgat gctgtttggc gtcgttttcc tctcccacca gctcggctcg
      241 ttcgccggct tgtggctggc gggcaccctt tacgatctga ccaagtccta cgatatgatg
      301 tggtggatct cgatcgccct gggcctgttc gcggccgccg tgcactggcc gatccaggag
      361 cgcccggtac cccggctcgt cgccgcgacc taggcgcagg ccaacactgg atccgggcgg
      421 caaaggtggc gagccattgg cgtcgccatc tgcacttata atggcgccga gctgtgatgc
      481 acggcgctgg acaaggagag gtcgacaagg agacgtcaac aaggagacgt cgacacggag
      541 acgtggacaa ggagacggat ggcacagcaa agcccaacgg ccgccacccc ctcggctgag
      601 cgacaggcgc ccagccccag catgccgcgc gcgctgcgta tttcgctttt tgctgccctg
      661 ggcgcgctgc tggcgggcgc cgcctatttg ctggtcgtgc gcggtgaggc cctgattgtc
      721 gatctcgccg ccgtctcgca gcgcattttc tgcttttagc cgcgccggcc ccaaccccgg
      781 gtaagcgcgt tcaatgctcg ggaactgact tgaaccgagg ccggagttgg caagccatga
      841 acgtgaggca ggcggtagcg atgggcggtg cggtggcgaa agctggtctc gcgattgccg
      901 ccctgttggc cctttgtgcg ccgccggcac tggcgcaaga cctgaaaact tatggcaaaa
      961 agggcagcta cgaggacgtc aaattcgaac tcaacgatgc catcatcagg cgcggccttg
     1021 ccatcgacta caccggccag atcaacaaga tgctggagcg caccggtgcc gatgtcggtt
     1081 caaccaagaa gatctacgcc aacgccgagt tcttcatgtt ctgctcggcc aaactctcgc
     1141 gcgacatgat cgaggccgac cccgccaacc tcggctattg tccctacgtg atatttctct
     1201 acgaagccgc cgataaacca ggcgaaatcg tcgttggcta tcgccgcccc tcgcccaagg
     1261 gcgacgcggc ttcgcagaag gcgctggccg ccatcgacgc gctgctcgac ggcatcgtca
     1321 aggacgccgc caagtagctc agcgacgccg gcgttgccgt tgcgccagcg cgcaagcaca
     1381 aaatatcctc cgtccacgta agcaattgac agctgcacgg cgcattgctt cgatccgatc
     1441 cgcgtgacgg tctcgtcgcg gtcccagttg gaccgcggcg gtgaaaaggg aacgcggtgc
     1501 gaggtcactc gatctcaaag ccgcggctgc ccccgcaact gtaagcggcg agcgtcgcac
     1561 ccgagtgcca ctgccgagcc aatcggcggg aaggcgggag cgatgcgcat ggagccgcga
     1621 gccaggagac ctgccggcac gagtcgccca tcaccgacgc ggggcgcgcc ggcgaacgga
     1681 tcaccgtagt ggcgacggct ctgtgttcga gttgagggtc gtgccggtga tgtctcaggt
     1741 gcaatcgcag cctgcaatgc tttagttggc ggcgccctcg gc
//
LOCUS       DASTMT010000642         3201 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_6192333, whole genome shotgun sequence.
ACCESSION   DASTMT010000642 DASTMT010000000
VERSION     DASTMT010000642.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3201)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3201)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3201
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6192333"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..285)
                     /locus_tag="VFR00_14785"
     CDS             complement(<1..285)
                     /locus_tag="VFR00_14785"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024913.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU0160586.1"
                     /translation="MPYQFRPLSTADQPLLRRWLAAPHVAEWWGDPDQQFALIREDAG
                     NAALEQYVVTVAARPIGYLQCYEVAAWPQGGLGSQPPGTCGIDQFIGEPDM"
     gene            complement(299..859)
                     /gene="hslV"
                     /locus_tag="VFR00_14790"
     CDS             complement(299..859)
                     /gene="hslV"
                     /locus_tag="VFR00_14790"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011942668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HEU0160587.1"
                     /translation="MNASSTSDAIWHGTTILTVRKAGRVVIAGDGQVSLGATVIKANA
                     KKVRSVGKGDVIGGFAGATADAFTLFERLEAKLEQYPGQLTRACVELAKDWRTDRYLR
                     RLEAMMIVADRSRTLTLTGTGDVLEPEGGVMGIGSGGNYALAAAKALMDGALGAEEIA
                     RRAMAIAAEICVYTNSNIVIESLDSA"
     gene            992..1588
                     /gene="hisB"
                     /locus_tag="VFR00_14795"
     CDS             992..1588
                     /gene="hisB"
                     /locus_tag="VFR00_14795"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HEU0160588.1"
                     /translation="MKRQASIERSTKETKIKASVDLDGTGAYDIATGIGFLDHMLEQL
                     ARHSLIDITLKAKGDLHIDFHHTTEDCGIVLGQAVAKALGSKEGIVRFSSLHLPMDET
                     LTRVAIDVSGRPYLVWRVQFSATKLGEMDTELFREFFQAFAQHAGLALHIETLYGDNN
                     HHIAETCFKGLARALGQAVAINPRQAGRVPSTKGTLSV"
     gene            1703..2176
                     /locus_tag="VFR00_14800"
     CDS             1703..2176
                     /locus_tag="VFR00_14800"
                     /inference="COORDINATES: protein motif:HMM:NF022400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2628 domain-containing protein"
                     /protein_id="HEU0160589.1"
                     /translation="MQTFTVHEPPSPAADRVDRAESLVFIKEGFSWGAALFGPFWLLA
                     HRLWWPLVGYVVAYGAIEAVRLARLIDQRWIGLVVFALNLLLAFEGDSLRRWALDRRG
                     WRLLGAANGRNRAECERRFFDAWLPAQPVLQAPAAAGGAGRRDWPVIGSILGARG"
     gene            2182..2832
                     /gene="hisH"
                     /locus_tag="VFR00_14805"
     CDS             2182..2832
                     /gene="hisH"
                     /locus_tag="VFR00_14805"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HEU0160590.1"
                     /translation="MQRVVIIDYGSGNLHSAAKAFERAARESGGDAQIVVSREPEAVA
                     SADRVVLPGVGAFADCKAGLAAVPGMIEALERCVRAKGRPFLGICVGAQLMAERGLEF
                     KITPGLGWLKGDVAAITPNDPALKVPHMGWNTLNVLQQHALLSGIRTGDGGLHAYFVH
                     SFHLVTVPGALLAETDYGGPVTAMVGADNIAGTQFHPEKSQTLGLRLIANFLAWRP"
     gene            complement(2901..>3201)
                     /locus_tag="VFR00_14810"
     CDS             complement(2901..>3201)
                     /locus_tag="VFR00_14810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0160591.1"
                     /translation="QAGRAKHSLSAAIMQLGEAWARPLETTWYVQSKERAAALEARLG
                     ALLDGEEGLLIQEVASEAVLLNTSLRWFRKRQANAGEVTNVVAFPLRAASEAKAA"
BASE COUNT          526 a         1058 c         1059 g          558 t
ORIGIN      
        1 catgtccggc tcgccgatga actgatcgat gccgcacgtt cccggcggct ggctgcccag
       61 tcccccttgc ggccatgcgg ccacctcgta gcactgcaaa tagccgatcg gccgggcggc
      121 aacggtcacc acatactgct cgagcgcggc gttgccggca tcctcgcgaa tgagcgcgaa
      181 ttgctggtcg gggtcgcccc accactcagc cacatgcggg gccgccagcc agcgcctgag
      241 caaaggctga tcggccgtgg acagggggcg aaactgatag ggcacgggcc ggcagtcctc
      301 aggcgctgtc gagactttca atgacgatgt tgctattggt gtagacgcag atctcggcgg
      361 caatcgccat ggcgcgccgc gcaatctcct cggcgccaag cgccccgtcc atcaacgcct
      421 tcgccgccgc cagcgcatag ttaccacccg accctattcc catgacaccg ccctcgggct
      481 cgagcacgtc cccggtgccg gtgagggtca acgtgcgcga gcggtcggcc acgatcatca
      541 tggcctccag acgccgcaag tagcggtcgg tccgccaatc cttggccagt tcgacacagg
      601 ccctagtgag ctgaccggga tactgctcga gcttggcttc gagccgttcg aacagggtga
      661 acgcatcggc cgtggccccg gcgaagccgc caatcacgtc gcccttgccg acggagcgaa
      721 cctttttggc gttcgccttg atgacggtag cgccaaggct gacctgcccg tcgcccgcaa
      781 tcaccaccct gcccgccttg cgcacggtca gaatggtggt gccgtgccag atggcgtcgg
      841 atgttgagga agcgttcatg cttcagccca tgtcggcgtg aacggggcgt aaatcaagtg
      901 ggttcagtat ttgcgctgga gcctatggca ggggcgggac caagctgata aaggcttgag
      961 cggacgcacg caaggtaagg cattaacctc cgtgaagcgg caagcgagca tcgagcgctc
     1021 caccaaggag acgaagatca aggccagcgt cgatctcgac ggtaccgggg cctatgacat
     1081 tgccaccggc attggctttc tcgaccacat gctggagcag ctggcccggc actccttgat
     1141 cgatatcacg ctcaaggcca agggcgattt gcatatcgat ttccaccaca ccacggagga
     1201 ttgtggcatc gtgttgggcc aggcggtggc gaaggccctt ggcagcaaag agggcattgt
     1261 acgcttttct tcgctgcacc tgcccatgga cgaaacgctg acgcgcgtgg ccatcgacgt
     1321 ctcgggtcgc ccctatttgg tgtggcgggt gcagtttagc gcgaccaagc tcggtgagat
     1381 ggatacggaa ttgtttcgcg aattcttcca ggccttcgcc cagcatgccg gtctggcgct
     1441 gcacatcgag accctttacg gcgacaacaa ccaccatatc gccgagacct gctttaaggg
     1501 acttgcccgc gcgctgggtc aggcggtggc gatcaacccg cgccaggccg ggcgggttcc
     1561 ctccaccaag ggcaccttga gcgtttgagc cggccggcgc cagacgtggg tccccggcac
     1621 gtgcgccctg gcaaagggct cgccaaggac ttcacgacca agtaaaaggc cctgccaacc
     1681 catcccggtg ccggccaatc ccatgcagac cttcaccgtg cacgagccgc ccagccccgc
     1741 cgccgaccgc gtcgatcggg cggaaagcct cgtcttcatc aaagaggggt tctcctgggg
     1801 tgcggcgctg ttcggcccat tctggctttt ggcccaccgg ctgtggtggc ctctcgtggg
     1861 ttacgtcgtg gcctatgggg cgatcgaggc ggtgcggctg gcgcgcctca tcgatcagcg
     1921 ctggattgga ctggtcgtct tcgccctcaa cctgctgttg gccttcgagg gcgatagcct
     1981 gcggcgttgg gcgctcgatc ggcgcggctg gcgcctgctc ggcgcggcca acgggcgcaa
     2041 cagggccgaa tgtgaacggc gcttcttcga cgcctggctt ccggcccagc cggttttgca
     2101 ggctcccgct gccgccggcg gcgccggacg gcgcgattgg cccgtcatcg gctctatcct
     2161 gggtgcgcgc ggctagcggc catgcaacgc gtcgtcatca tcgattacgg ctccggcaat
     2221 ctgcattccg ccgccaaggc cttcgagcgg gcagcgcgcg aaagcggcgg cgatgcgcaa
     2281 attgtcgtca gccgggagcc ggaggccgtg gccagcgctg atcgggtggt cctcccgggc
     2341 gtcggcgcct tcgcggattg caaggcgggg ctcgccgccg tgccgggcat gatcgaggcg
     2401 ctggagcgct gcgtgcgcgc caaggggcgg ccctttctcg gcatttgcgt gggcgcccag
     2461 ttgatggccg agcgcgggct cgaattcaaa atcaccccgg gactgggctg gctcaaaggc
     2521 gacgtcgcgg caattacccc caatgacccc gcgctcaaag ttccgcacat gggctggaac
     2581 acgctgaacg tgctgcagca gcacgccctg ctcagcggta tcaggaccgg ggatggcggc
     2641 ttgcacgcct atttcgtgca ttccttccac ctcgtcaccg tccccggggc attgctggcg
     2701 gaaacggact acggcggacc ggtgaccgcg atggtcgggg cagacaacat cgcgggcact
     2761 cagttccatc cggagaagag ccagacgctc ggacttcggc tcatcgccaa ctttctggcc
     2821 tggcggccgt gaggcggcac cgccccgccg ctgggggaaa cgcgcggggg gtttgggggc
     2881 ggggcctgcc tcgccggtga tcaagccgcc ttggcctcgg acgcggcacg caacgggaac
     2941 gctacaacat tcgtgacttc gccggcgttg gcctggcgct tgcggaacca acgcagcgac
     3001 gtattgagca gcaccgcttc gctcgcgacc tcctggatca gaagaccctc ctcgccgtcg
     3061 aggagagcgc cgaggcgggc ttccagcgcc gcggcgcgct ctttggactg cacgtaccag
     3121 gtggtctcga gagggcgggc ccaggcctcg ccgagctgca tgatggcggc cgacaggctg
     3181 tgtttggccc ggccggcttg c
//
LOCUS       DASTMT010000643         3251 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_5209279, whole genome shotgun sequence.
ACCESSION   DASTMT010000643 DASTMT010000000
VERSION     DASTMT010000643.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3251)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3251)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3251
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5209279"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..802
                     /locus_tag="VFR00_14815"
     CDS             <1..802
                     /locus_tag="VFR00_14815"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEU0160592.1"
                     /translation="ECDIVEHFTIAYYFERFADATRNFFELVDEAERLSSVVEARPSR
                     LYTSFSQELRAGAGFHILSAVVGLDESSLRLGHQVIYSSRAETVSWVMEILPLPASAT
                     PELRRRLEANVVAWPGPQVPAAPRTARRGALTARDRVKPRELGEDGGMALAAHVHRFS
                     GAGMQFLSSIGMTGDYMHQNRRGFSTLALDLRLSGVAGVGDRIDVETGIAHLGNTSLS
                     YLHRMRGADGREIASMLQTGVQLDLDARRPAPLPPVIREAVSRLLAQA"
     gene            834..2048
                     /locus_tag="VFR00_14820"
     CDS             834..2048
                     /locus_tag="VFR00_14820"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HEU0160593.1"
                     /translation="MTNADGPLKGVKVVACSTAQAGTVPYMLMADLGAEVIKIEVPEG
                     GDPQRVSSVLPGHPSSFFETNNRGVKSVTLNLKTPEGRKILRQLVAGADIFGQNFRPG
                     AAEKNGFGYEELKQVNPKLVYISISGYGPKGPNAHLPGTDSMAQALGGIAQAYAAPGQ
                     PMRTGIASVADETCAILAFGGALAALTHARATGVGQKVELSLLGSIVRLLGWTFTTTL
                     WRGTDPVTGQARVAGTRERPAISASFNDRDGKPMVFQLGGDQWQKAMVGLGFYGEVEK
                     AGFGHLGEAMDSAERRAELLALLDRLFAANTREHWVAKLRAADIVSAPINTMLEAAAD
                     PDVLANGYVTEVDYPEHGKRLRVHGTPWQFSETPAKIGIAPDLGQHNDEVLTSLGYGT
                     ADIQGFRERKII"
     gene            complement(2041..2841)
                     /locus_tag="VFR00_14825"
     CDS             complement(2041..2841)
                     /locus_tag="VFR00_14825"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEU0160594.1"
                     /translation="MAVVESERHGQVLVLRMNRPERLNALNSEMRTALAELWTEFRHD
                     KDLEVAIFTGTGRGFCAGEDMKESLASGTPGGTRPTIPDPFMDGTLEKPVIAAVNGFA
                     MGGGFMLVERTDLRLAVPEAVFEVSEAKRWLLGGYNHGHIANLPYPVAMEMALGFRFP
                     AERLYQVGFLNRLVPQAELMPAALAMAEHLLSLPPAARVNTVHMMRQMRPEPSPDHKA
                     LAAKLHEHGDKRDLMESRAAFAEKRKPNFKGWLDPQDRYRLPGLEDVK"
BASE COUNT          563 a         1067 c         1054 g          567 t
ORIGIN      
        1 ggaatgcgat atcgtggagc atttcacgat cgcctactat tttgagcgct ttgccgatgc
       61 gacacgcaat tttttcgagc ttgttgacga ggcggaaagg ctgagcagcg tggtcgaggc
      121 tcggccatcg cgtctttaca ccagctttag ccaggagttg cgcgccggcg ccggcttcca
      181 catcttgagc gcggtcgttg gcctcgacga gagttcgctg cggcttggtc atcaggtcat
      241 ctattcgtca cgggccgaga ccgtgtcctg ggtcatggaa atcctgccgt tgccggcgtc
      301 ggcaacgcca gagctgcgtc gccggctcga ggccaacgtc gtggcctggc cgggacccca
      361 agtacccgcg gcgccgcgca cggcaaggcg cggtgccctc accgcccggg accgcgtgaa
      421 gccgcgggag ctcggcgagg atggggggat ggcgcttgcc gcgcatgtgc atcggttttc
      481 cggggcgggc atgcagtttt tgtcttcgat cggcatgacc ggcgactata tgcatcagaa
      541 ccgccgcggc ttctcgaccc tggcgctgga cctgagactg agtggggtcg caggggttgg
      601 cgaccgcatc gacgttgaga ctgggatcgc ccaccttggg aacacctcgc tcagctatct
      661 gcatcgcatg cgtggcgcgg atggtcgcga gatcgcgtct atgctgcaga ccggcgtaca
      721 actcgacctc gacgcgcggc ggccggcgcc gctgccgccg gtcatccggg aggcggtctc
      781 gaggttgtta gcgcaggcct agacgagttc agttcagaaa cggggagaag ccaatgacca
      841 atgccgatgg cccgctgaaa ggtgtgaagg tcgttgcctg ctcgacggca caagccggca
      901 cggtgcccta catgttgatg gccgatctcg gcgccgaagt catcaagatc gaggtacccg
      961 agggcggcga tccccagcgc gtctcctcgg tcctaccggg gcatccaagc tcgttctttg
     1021 agaccaacaa ccgcggtgtt aagagcgtca cgctcaatct caagaccccc gagggtcgca
     1081 agatcctgcg ccagctcgtg gccggtgccg atatcttcgg tcagaacttc cgcccaggcg
     1141 cggcggagaa gaatggcttt ggctacgaag agttaaagca ggtcaacccg aagctcgtct
     1201 acatctcgat ttccggctat ggcccgaagg gacccaacgc gcatttgccg ggaacggact
     1261 ccatggcgca ggcgcttggc ggcatcgccc aggcctatgc tgcgccgggc caaccgatgc
     1321 gcaccggcat cgcctccgtt gccgatgaaa cctgcgccat tcttgccttc ggcggggcgc
     1381 tggcggcctt gacgcatgcg cgcgccaccg gcgtcggcca aaaggtcgaa ctatcgctgc
     1441 ttggcagcat cgttcgcctg ctgggctgga ccttcacgac gaccttgtgg cgcggcaccg
     1501 acccggtgac gggccaggcc cgcgtcgccg gcacgcgcga gcgcccggcg atctcggcga
     1561 gcttcaatga tcgtgacggc aagccgatgg tgttccagct cggtggcgac caatggcaga
     1621 aggcgatggt cggcctcggc ttttatggcg aggtggagaa ggcaggtttt ggccacttgg
     1681 gtgaggccat ggacagtgcc gaacggcggg ccgagctgct cgccttgctc gaccgtctgt
     1741 tcgcggccaa cacgcgcgag cactgggtgg ccaagctcag ggccgccgat atcgtctcgg
     1801 cgccaatcaa cacgatgctc gaggcggccg cagaccccga tgtgctcgcc aacggctacg
     1861 tcaccgaggt cgactatccc gagcacggca agcgtttgcg ggttcacggc acgccctggc
     1921 agttctccga aacaccggcc aagatcggga ttgcgcccga cctcggccag cacaacgacg
     1981 aggtcctgac gagcctcggc tatggcacgg cggacattca ggggttccgg gagcgcaaga
     2041 tcatttgacg tcctcaagac ctggcaggcg atacctgtct tgggggtcga gccaaccctt
     2101 gaagttgggc ttgcgcttct cggcaaaggc ggcgcgcgat tccatcaggt cgcgcttgtc
     2161 gccgtgctca tgcaacttgg cggccagcgc cttgtggtcc ggagaaggct ccggacgcat
     2221 ctgccgcatc atgtgcacgg tattcacgcg cgcggccggt ggcagcgaca gcaggtgctc
     2281 ggccatggcc agggccgccg gcatgagctc ggcctgcggc acgagccggt tgaggaagcc
     2341 gacctggtac aagcgctcgg ccgggaagcg gaaaccgagc gccatctcca tggcgacggg
     2401 gtagggcaga ttggcgatat ggccatgatt gtaaccaccc agcagccagc gtttcgcctc
     2461 tgaaacctca aacaccgcct ccggcacagc caggcgcaga tcggtgcgct cgaccagcat
     2521 gaagcccccg cccatggcaa agccgttgac ggcggcgatc accggctttt ctagcgtgcc
     2581 gtccatgaaa ggatcgggaa ttgtcggccg cgtgcccccg ggcgtgccgc tcgcaaggga
     2641 ttccttcatg tcctcgccgg cgcagaagcc acgtcccgtg ccggtgaaga tcgcaacctc
     2701 caagtccttg tcgtgccgga actcggtcca cagttcagcc agggccgtgc gcatttcgct
     2761 attgagggca ttgagccgct ccgggcggtt catgcgaagg actaggacct gtccgtgacg
     2821 ctcgctttcg acgacggcca tgcgaggatc tccacttggt ttcgtgcggc tgcggccgcc
     2881 aaagcgaagt ctagaccgat ccccccgccc actgaaggcg acagggccgc cgctgctgac
     2941 gcggctaacg gccatgcgcc ttcgcacaac agccgccaca agttgcgagg acaccgcgtg
     3001 cgcgtcaagt cgtccaggtc cgcaacgtga ggcggccctc aactctggcg cggtcgcctt
     3061 gcgaaactac gaacgacggg gcctatcggc cgagacggca aggaccctcg gactcagtac
     3121 ccaccgccgc cacctccgcc cccaccaccg ccgccgccac ccccaccacc gtcgccgccc
     3181 ccaccggact gcctctcctg atttctccag aagtctttga gccaggcgcc gctggcgtcc
     3241 gaagtggctt t
//
LOCUS       DASTMT010000644         3830 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_3886153, whole genome shotgun sequence.
ACCESSION   DASTMT010000644 DASTMT010000000
VERSION     DASTMT010000644.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3830)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3830)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3830
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3886153"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..778
                     /gene="dapE"
                     /locus_tag="VFR00_14830"
     CDS             <1..778
                     /gene="dapE"
                     /locus_tag="VFR00_14830"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015596261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HEU0160595.1"
                     /translation="RRGSLSLLITGDEESVAINGTRKLLDWLKQRQEKIDGCLVGEPT
                     SAEALGDEVKIGRRGYLNASLVVHGKQGHSAYGDKAENPIPKLARIIDRLATAVLDNG
                     TEHFQRTSLEPTIISVPNTATNVIPGTARANFNIRYNDLHTRQSLQAWLQEQCQAAAA
                     EVGGKFSLSFEGNGDVFLTKPGPLVEMMTAAVQAITGHRPALTTNGGTSDARFIKELC
                     PVVEFGLRNATAHQIDEHVPIADLQALTAIYRRFIELFLA"
     gene            complement(891..2222)
                     /locus_tag="VFR00_14835"
     CDS             complement(891..2222)
                     /locus_tag="VFR00_14835"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HEU0160596.1"
                     /translation="MRTSDQVLAELKALHPRRIDLSLGRIERLLGRLGDPHNRLPPVI
                     HVAGTNGKGSLTAYLKAMLQAAGRRVHVYTSPHLVRFHERIELAGAGARAAPIGEGAL
                     VDYLTRVEQANAGDVVTFFEITTAAAFLAFAEHPADALILEVGLGGRLDATNVVSRPA
                     LSVITPISLDHTEWLGATVDEIAREKAGILKPGVAAVISQQPFEALNAIAALAEAIGA
                     PLTIWGQDYEAFEQRGRLVYQSAEQLMDLPLPALIGQHQIVNAGTAIAATQHLKALGL
                     GQAPTIERGLVEVRWPARMQRLTKGPLAQRVDNGSELWLDGGHNAAGGAAIAQTLGEL
                     EERAPKPVGLVLGMLDTKDAAGFLAPFTGLVRHVRTVPIAGTEAAQDAAALAGVAAKV
                     GLAARPAADVESAVVELMAAEPAPIRILICGSLYLAGQVLAQQDGVQAQAN"
     gene            complement(2330..3418)
                     /gene="accD"
                     /locus_tag="VFR00_14840"
     CDS             complement(2330..3418)
                     /gene="accD"
                     /locus_tag="VFR00_14840"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HEU0160597.1"
                     /translation="MNWINNVVRPKIRSLLTTKRDLPDNLWVKCPDTGQMVFYKDLEA
                     NQFVIPGSEHHMRMGAQQRLQHLFDDGQYERVPVAGVAQDPLKFRDGRRYSDRLKTAK
                     ADTELDDAVLVGEGNLEGSPVVAAAQDFRFMAGSLGMAAGEAVIAGMLRAVESRAPFI
                     LFAASGGARMQEGILSLMQMPRTTIAVQKLREARLPYLVVLTDPTTGGVTASYAMLGD
                     VHIAEPGALICFAGPRVIQQTIREQLPEGFQKAEYLLAHGMIDMVIHRHKMRETLARL
                     CRLLTATEKTVTQRKRPVAAKHEDRAYLNGSVVDGQTIETLARGSDAMEPEPVDDNER
                     FEVRPKTTRHDQEAKPLPRPGSRDGARN"
     gene            complement(3502..>3830)
                     /locus_tag="VFR00_14845"
     CDS             complement(3502..>3830)
                     /locus_tag="VFR00_14845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="geranylgeranylglyceryl/heptaprenylglyceryl
                     phosphate synthase"
                     /protein_id="HEU0160598.1"
                     /translation="VVLTHTSGFLYYVSITGITGTAAPDVADVHAQVARIKKSTNLPV
                     VVGFGVKTPEQARRIALGADGVVVGSALVNAVRDSLDREGKATGNTVPDVLNLVRNLS
                     GALRPA"
BASE COUNT          691 a         1288 c         1182 g          669 t
ORIGIN      
        1 gcggcgtggg tccctgtcgt tgctgattac cggcgacgag gagtcggttg ccatcaacgg
       61 cacgcgcaag ctgctggatt ggctgaaaca gcggcaggaa aaaatcgatg gttgtctggt
      121 gggcgaacca accagcgccg aggcactggg cgatgaagtg aaaattggcc gccgcgggta
      181 cctcaacgcc tcgcttgtcg tgcacggcaa gcagggtcat tccgcctacg gcgacaaggc
      241 cgaaaatccg atcccaaagc tggctcgcat catcgatcgc ctcgcgactg ccgtcctcga
      301 caatggtaca gagcactttc aacggaccag cttggagccg accattatat cggtaccgaa
      361 cacagccacc aacgtcatac ccggtaccgc ccgcgcaaac ttcaacatcc gctacaacga
      421 tctgcacacc cgccagagcc ttcaggcctg gctgcaggag cagtgccagg ccgcggccgc
      481 tgaagtcggc ggcaagttct cgctctcttt cgaaggcaac ggcgatgtgt tcctcaccaa
      541 gcccgggcct ttggtcgaga tgatgacggc ggcggtgcag gcgatcaccg gtcacagacc
      601 ggccctgacc accaatggtg gaacctccga tgcgcgcttc atcaaggagc tttgtcccgt
      661 ggtcgagttc ggattgcgca acgccacggc ccatcagatc gacgagcacg taccgatcgc
      721 ggatttgcag gcgctgacgg ccatctaccg gcgatttatc gagctgtttt tagcctgacg
      781 acccgcggcg gcaggcgccc tgggcgggca tgcggcaccc aagctgaggc accaccgcaa
      841 cctgccgcaa cggttttgag ccgccgtcgt cgccggcggc caaggggggc tcaattggct
      901 tgtgcttgca cgccgtcctg ctgcgccaag acctggccgg ccaggtacag cgaaccgcag
      961 atcaagatgc ggatgggggc tggttccgct gccatcagtt cgacgacggc gctttcgacg
     1021 tcggcagcag gacgcgcggc caggccgacc ttggcggcga ccccggcaag cgcggcagca
     1081 tcttgcgccg cttccgtccc ggcgatcggc accgtgcgaa cgtgccggac caggccggtg
     1141 aacggcgcaa gaaagccggc ggcatctttg gtgtccagca tgccaagcac caagccgacg
     1201 ggcttgggcg cgcgttcctc gagctcgccc agggtctgcg cgatggccgc gccgccggcc
     1261 gcattgtgcc cgccgtcgag ccacagctcg gaaccgttgt cgacccgttg cgccaatggc
     1321 cctttggtca gtcgctgcat gcgcgcgggc cagcgaacct caacaagccc gcgttcgatc
     1381 gtcggcgctt gcccgagccc cagcgccttg aggtgctggg tcgcggcaat ggctgtgccc
     1441 gcgttgacga tctgatgctg cccgatcaag gcgggcagcg gcagatccat gagctgctcg
     1501 gcgctttgat agaccaagcg accccgctgc tcgaaggcct cgtagtcctg accccagatc
     1561 gtcaagggcg cgccaatcgc ctcagcgagc gcggcaatgg cattcaaggc ctcgaacggc
     1621 tgctgcgaga tcaccgcggc cacgccgggc ttgaggatgc ccgccttttc acgggcaatc
     1681 tcatcaaccg tcgcacccaa ccactccgtg tgatccaacg aaatcggcgt aatgaccgag
     1741 agcgccgggc gagacacgac attggtggcg tcgagccgtc cgcccagccc gacctccagg
     1801 atcagggcat cggccggatg ctcggcaaag gcgagaaatg ccgccgccgt cgtgatctca
     1861 aaaaacgtca cgacatcgcc ggcgttggct tgctcgaccc tcgtcaggta gtccaccagc
     1921 gcaccctcgc cgatcggtgc ggcccgggcg ccggcgccgg ccagttcgat acgctcgtgg
     1981 aagcgaacga ggtgcggcga agtgtagacg tgcacgcgcc ggcccgccgc ctgcaacatc
     2041 gcctttaggt aggccgtcag cgaccccttg ccgttcgtac cggcaacgtg gatcacgggc
     2101 ggcagccggt tgtgtggatc gcccagccgc cccaaaagcc gttcgatgcg gccgagcgat
     2161 agatcgatgc gccgcggatg cagcgccttc agctctgcca atacctgatc gctcgtgcgc
     2221 atgcggcgcc cttatcacaa acccattgtg gcacaacccc aagcacccgc gcgccgcgat
     2281 cgcccgtcac cggttccacc gtaaagccgg gactggcgcc cgccgaccgt cagttgcggg
     2341 caccgtcacg ggaaccggga cgcggcagcg gcttggcttc ttgatcgtgc cgcgtggtct
     2401 tgggacgaac ctcgaagcgc tcgttgtcgt cgaccggttc aggctccatg gcatcggagc
     2461 cgcgggcaag cgtctcgatg gtttgaccgt ccacgacact gccattgagg taggcgcgat
     2521 cctcgtgttt ggctgctacc ggccgcttgc gctgcgtgac ggtcttttcc gtcgccgtca
     2581 gcagacggca cagcctggcc aaggtttcgc gcattttgtg gcggtgtatg accatatcga
     2641 tcataccatg cgccagcaga tactcggcct tttggaagcc ttcagggagc tgctcacgga
     2701 tggtttgctg aatgacgcgc ggcccggcaa aacagatcaa cgcaccaggt tcggcaatgt
     2761 gcacgtcacc cagcatcgca taggaagccg tgacgccacc cgtcgtcgga tctgtcagca
     2821 cgaccaggta gggcaggcgc gcctcgcgca gcttctgcac ggcaatcgtt gtgcgcggca
     2881 tttgcatcag agaaaggatg ccctcctgca tgcgcgcgcc acccgaggcg gcgaacagta
     2941 tgaacggcgc ccggctttcg acggcgcgca gcatccccgc aatcaccgcc tcaccggccg
     3001 ccatgccgag cgagccggcc atgaaacgaa aatcctgggc ggccgccacg accggcgagc
     3061 cttcgagatt gccctcgccc accagcacgg cgtcatcgag ctcggtgtcg gcctttgccg
     3121 tcttcaggcg gtcgctgtag cgccgtccgt cgcgaaactt gagtggatcc tgcgccaccc
     3181 ctgcaaccgg cacccgctcg tactggccat cgtcgaacag atgctgcagc ctttgctgcg
     3241 cccccatgcg catatggtgc tcggagccag ggatcacgaa ctgattggcc tcgaggtctt
     3301 tgtagaagac catttgcccc gtatcgggac atttcaccca aaggttgtct ggcagatccc
     3361 gcttggtcgt gagcaggcta cggatcttgg gacgaacgac gttattgatc cagttcacgc
     3421 ctcagtgctc cttgggcact tcccgcagtc tgcgcctggg ccaccctcca gtccacgccg
     3481 ccgcgccggc ttgaactgcc attaggcggg gcgcaatgcc ccggacaagt ttctcaccag
     3541 atttaggacg tctgggaccg tatttccagt cgctttgcct tcccgatcaa ggctatcgcg
     3601 gacggcatta accagggcgg agccgaccac gaccccgtcg gcgcctagcg caatccgccg
     3661 cgcctgctct ggggttttca caccaaaccc caccaccacg ggcaagttgg tcgatttttt
     3721 gattctcgca acctgcgcgt gcacatcggc cacgtcgggg gccgccgtgc cagtaatgcc
     3781 cgtgatcgac acgtagtaga ggaagcctga ggtgtgcgtg agcacgactg
//
LOCUS       DASTMT010000645         9257 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_6459746, whole genome shotgun sequence.
ACCESSION   DASTMT010000645 DASTMT010000000
VERSION     DASTMT010000645.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 9257)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9257)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9257
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6459746"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(73..2241)
                     /gene="uvrC"
                     /locus_tag="VFR00_14850"
     CDS             complement(73..2241)
                     /gene="uvrC"
                     /locus_tag="VFR00_14850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013421033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEU0160599.1"
                     /translation="MPSKPDPDSSLAVTAAPAAPAARSETGAGVIARYLTSLPGGPGV
                     YRMIDAAGTVIYVGKARNLKARVTNYARAGNHPARIMRLIAATAAMEFVTVRTEAEAL
                     LLEANLIKRFRPRYNVLLRDDKSFPYILIATAHRAPQILKHRGARNRKGDYFGPFASA
                     GAVNRTINMLERAFLLRSCSDAVFESRTRPCLLYQIKRCSAPCTGLIGLEDYNGLVEE
                     ARRFLRGESENARKMYQRLMEEASASLDFERAAAYRNRLWALAHVTADQSINLEGIEE
                     ADVFAAHQDAGQVCIQVFFFRSGQNWGNRAYFPRADRTLGVEEVLESFIAQFYDDKPV
                     PSCILLSHKLPNEALLAEALSTKAERKVEITTPQRGAKVGIVEHAAQNAREALARRLA
                     ESSTQRQLLEGLAQRFSLPVTPRRIDVFDNSHIMGANAVGAMIVAGAEGFVKAQYRRF
                     NIKSSDLAPGDDYAMMREVLTRRAKRLLLEATGAKPAIAQAAPAEPPAEAAVSRLAAP
                     QPPPIDRRGGSALAFGVLRADEVEAAVTGSTPGLAETEQETLEGNAQEMNLPETDGTG
                     DEFPAKPDLLLIDGGLGQLGVAQEVIETFGIQGVALIGVAKGPDRDAGREHFHLPGRA
                     SPMMLEPRDPVLYFVQRLRDEAHRFAIGTHRARRARALGANPLDEIPGVGPGRKRALL
                     KHFGSAKAVSRAGKEDLAAVQGISAQMAQTIYDFFHERVE"
     gene            complement(2496..3383)
                     /locus_tag="VFR00_14855"
     CDS             complement(2496..3383)
                     /locus_tag="VFR00_14855"
                     /inference="COORDINATES: protein motif:HMM:NF021230.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TorF family putative porin"
                     /protein_id="HEU0160600.1"
                     /translation="MNVRSPALVAPTAAAIALALLAGTAHADGMARGSIKDAPPPPPQ
                     ERCKLSANIGLATEYVFRGISQTSEGPAVQGGFDATCGMFYAGVWASNLDWGANDGTN
                     IANIEMDWYAGIKFNTGRIAWDVGVIYYTYPRGADLAVVSLTDPTLVKAEFNYVELKV
                     GASAELWKDGTLGVTGFFSPDYQYETGNVWTVEGSFTQNLPKFMAFGREWSPSVSALI
                     GYQAAAGSTAEKARYAGNVTGDDDHYVYWNAGLTLGFLDKWSLDFRYWDTNIDRADDC
                     KGPLFACDERFVATLKFTY"
     gene            3694..4461
                     /locus_tag="VFR00_14860"
     CDS             3694..4461
                     /locus_tag="VFR00_14860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012111198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU0160601.1"
                     /translation="MADETPPTVLITGAAQRIGRALAGDFARRGWRVAIHCRASQRQA
                     QELAQELGRSGTASVVLPADLADAGAVAGLIAECSRRLGPPTCLINNAAMFAPDAIGE
                     LDGALWDTQLAVNLKAPVFLAEAFAKELPPGVAGNVINIIDQGVLKPTPQFFSYTVSK
                     AALWAATRTLAQALAPRIRVNAIGPGPVLKSAHQTEAQFRAEYEATLLERSTRPEEIA
                     AAIRFILEAPALTGQMLALDGGQHLAWQTPDVTLGDR"
     gene            4467..4880
                     /gene="folB"
                     /locus_tag="VFR00_14865"
     CDS             4467..4880
                     /gene="folB"
                     /locus_tag="VFR00_14865"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HEU0160602.1"
                     /translation="MATAPPTTAPEAAKPARPSGSRRVFVRDFEIVASVGVFEHEKRY
                     EQRILLSADLAVRDDYDGVSDRLEDVLDYSTLVEGIALLVQQEHVNLLETLAERIARH
                     CLADRRVESVRIRIEKPDALPMCRSVGIEIERGRA"
     gene            5277..6581
                     /locus_tag="VFR00_14870"
     CDS             5277..6581
                     /locus_tag="VFR00_14870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0160603.1"
                     /translation="MPRRVGAFLLFALLAAPCPLAAADLPAIKTNRSNAVPACVTPGR
                     LLAFLESRNPQLDPRFRSIAGDYMLLGDRLGVRWDYAFYQMLLETGSLSFRDGNRSGD
                     VKAQQNNFAGLGATGRGEHGESFKDIATGVRAHLEHLLLYAGERVENPVAERTRKVQE
                     WDVLTDWHKGFTRPVTFGDLAAKWAPGTRSYRRMIEGIAERFEEFCARPDPKPELMQE
                     ARAHKDKRESKPSEAKPERAGGNELAQRAIEAGKAESNEKRLGLGVQANAAAATSATV
                     PLRMLNQPAAEPGKEAGPAAAIAADDGGGAKLERDRLAGAITASTAKPLAPGKTDKSG
                     GADKSLQTASTAAAAKAALSPAAGQKCRVWTASYGGQKAMIIKSQGDKVVNYTVLDVN
                     EGSEAREAEAFISAYAKNGAIAGQFGSQAQALEKAFELCPEG"
     gene            6832..8076
                     /locus_tag="VFR00_14875"
     CDS             6832..8076
                     /locus_tag="VFR00_14875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006612481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HEU0160604.1"
                     /translation="MLKLFARLPLLGLILIAAQALPAAGEQNRAAVPNNPNCRNTGSF
                     DAWLDEFRQEARRNNISSATIAAALGGMTLDPGIIQRDRRQGFFAQTFTAFSGKLITP
                     NRIQNGLGRLKQHRDLLARIENQWGVPGPVIVAFWGLESDFGSMMGNLPVLRSLATLA
                     YDCRRPDMFRAELMEALRIIDRGDLRPENMIGSWAGELGQTQFLPAHYMQHAVDYDGD
                     GRRDLLQSVPDVLASTANFIAFLGWQRGQPWLDEVRLTRDLPWAEADLAIQHPVEKWA
                     GWGVKSVSGRPLSSTAATASLLLPMGRHGPAFLAYPNFQVYLKWNQSLNYSITAAHLA
                     DRLAGAPAMDKGNGTVPEMSLQQVKDLQGLLAKRGYFSGEVDGKIGAATRAAVKAAQL
                     KLGLPADSYPTPELLERMQAMR"
     gene            complement(8086..8388)
                     /locus_tag="VFR00_14880"
     CDS             complement(8086..8388)
                     /locus_tag="VFR00_14880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0160605.1"
                     /translation="MGKRPSKQRWHRRMRLLGPRRAAFAGLVLGAALWNFASLYELVG
                     GLMVGASAGPGEAASEGCTSLQLDRPTGRTQAWPCPEAAAWMESALRPPLTSAGRP"
     gene            complement(8572..>9257)
                     /locus_tag="VFR00_14885"
     CDS             complement(8572..>9257)
                     /locus_tag="VFR00_14885"
                     /inference="COORDINATES: protein motif:HMM:NF012659.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ribonuclease T2"
                     /protein_id="HEU0160606.1"
                     /translation="WGSEHRAGAFDYYVLALSWSPTYCSDVGDTRYDPQCRPGGARPY
                     AFVLHGLWPQYERGWPEHCRSPDRGYVPRPVANRMLDIMPSDKLIFNEYRKHGTCSGL
                     GVDGYFALARQLYDKVRIPERFVGLSDERLLLSPGELVREFVAANPGLEPDMLAVDCG
                     GTGHRLREVRVCFDRGGAFRRCGGNENPRRLCTAERMYVPPVRAGSGARVPSSPAEIV
                     PGPREDRRR"
BASE COUNT         1535 a         3142 c         3110 g         1470 t
ORIGIN      
        1 ggtgcagatg gagcccaacc tggtggtcga gttctacagc cggtaaggtg tcagaccctc
       61 cgggtctgac acctactcca cccgctcgtg gaagaagtcg tagatggtct gggccatctg
      121 ggcgctgatg ccctgtacgg ccgcgaggtc ctccttgccg gcgcgcgaga ccgccttggc
      181 cgagccgaag tgcttgagga gcgcgcgctt gcggcccggg ccgacgccgg gaatctcgtc
      241 gagcggattg gcgccgagcg ccctggcgcg cctggcgcgg tgcgtgccga tggcgaagcg
      301 gtgcgcctcg tcgcgcagcc gctgcacgaa gtagagcacg gggtcgcgcg gctccagcat
      361 catcggcgag gcgcggcccg gcaggtggaa atgctcgcga cccgcatccc ggtccggccc
      421 cttggcgacg ccgatcagcg ccaccccctg gatgccgaat gtctcgatca cctcctgcgc
      481 cacgccgagc tgacccaggc caccgtcgat caacagcagg tccggcttgg cggggaactc
      541 atccccggtc ccgtccgtct ccggcaggtt catctcctgc gcattgcctt ccagcgtctc
      601 ctgctccgtc tcggccaagc ctggggtcga gcccgtcaca gcggcctcca cctcatcggc
      661 ccgcagcaca ccgaaggcga gcgcgctgcc cccgcgccgg tcgatgggcg gcggttgcgg
      721 tgccgccagg cgcgagacgg cggcctcggc tggcggctcg gccggcgccg cttgggcgat
      781 cgccggcttg gccccggtcg cctcgagcag caaccgcttg gcgcgtcgcg tcagcacctc
      841 gcgcatcatg gcgtaatcgt cacccggcgc gaggtcgctg gatttgatgt tgaagcgccg
      901 atactgcgct tttacgaaac cctccgcgcc ggcgacgatc atggcgccca ccgcattggc
      961 gcccatgatg tggctgttgt cgaacacgtc gatgcggcgc ggagtgacgg gcaaggagaa
     1021 gcgctgagcc aagccttcga gcaactggcg ttgtgtggaa ctctccgcca gccgtcgcgc
     1081 caaagcctcg cgcgcgtttt gtgcggcatg ctcgacgatg ccgaccttgg ccccgcgctg
     1141 cggcgtggtg atctccacct tgcgctcggc cttggtggag agcgcctcgg ccagcagcgc
     1201 ctcattgggc agcttgtgcg aaagcagaat gcacgagggc accggtttgt cgtcgtagaa
     1261 ctgggcgata aagctctcca gcacctcctc gacccccagc gtgcgatcgg cgcgggggaa
     1321 ataggcccgg ttgccccagt tctgtcccga gcggaaaaag aacacctgaa tgcagacctg
     1381 tcccgcgtcc tgatgcgcgg cgaacacgtc ggcttcctcg atgccctcca ggttaatgga
     1441 ctgatcggcg gtgacatggg ccagcgccca caagcgattg cggtatgcgg ccgcccgctc
     1501 gaaatcgaga ctggccgagg cttcctccat cagacgctgg tacattttgc gcgcgttctc
     1561 gctctcgccg cgcaaaaaac gccgtgcctc ctcgaccagg ccgttgtagt cctcaaggcc
     1621 gatcagccca gtgcaagggg ccgagcagcg cttgatctgg tagagcaggc atgggcgcgt
     1681 gcggctctcg aacacggcat cggagcagga gcgcagcaga aaggcgcgct ccagcatgtt
     1741 gatggtgcgg ttgacggcac ccgccgaagc gaaggggccg aagtagtcgc ctttgcgatt
     1801 gcgggcgccc cgatgcttga ggatttgcgg tgcccggtgc gcggtggcga tcagaatata
     1861 gggaaacgac ttgtcgtcgc gcagcagcac gttatagcgc ggacgaaagc gcttgatgag
     1921 gttcgcctcc agcagcaggg cctccgcctc cgtccgcacg gtcacgaatt ccatggccgc
     1981 ggttgctgcg atcaggcgca tgatgcgcgc ggggtgattg ccggcgcggg catagttcgt
     2041 cacgcgcgcc ttgaggttgc gcgccttgcc cacgtagatg acggtgccgg ccgcatcgat
     2101 catacgatag acgccggggc cgccgggcaa gctcgtcagg tagcgggcga tgacgccagc
     2161 cccggtctcg gaccgcgccg ccggcgcggc gggcgcggcg gtgacggcga gggagctgtc
     2221 cgggtcaggc ttgctgggca tgggcagtga cttaaggggg cgccggcccg aggtccagtg
     2281 ccgcggacgc tgccgccccc gcgcaagggg gtagccgccg cggcccggca agggcgctgt
     2341 gggatggatg gacagccccc acgggtatcc gagcgcggcg gccccctaac ggcggcccaa
     2401 caatcccgcg gcaaggccgc gccatcccgt ggcggcacgc caatgcaaaa cgggcccccg
     2461 caggggcccg ccgtcacgct cgccaagcgc gagcgttagt aggtgaattt aagcgtcgcc
     2521 acgaagcgct cgtcgcaggc gaagagcggg cccttgcagt catccgcgcg atcgatattg
     2581 gtgtcccaat atctgaaatc cagggaccac ttatcgagga agcccagcgt caagccggca
     2641 ttccaataga cgtagtggtc atcatccccg gtgacgttgc cggcgtagcg cgctttctcg
     2701 gccgtgctcc ccgccgccgc ctggtagcca atcagggcgc tgacgctcgg cgaccattcg
     2761 cggccgaacg ccatgaactt cggcaggttc tgggtgaagg agccctccac cgtccacacg
     2821 ttgccggtct catactggta atccggcgag aagaagcccg tcacgcccag cgttccgtct
     2881 ttccagagct cggcgctggc gcccaccttc agctcgacgt agttgaattc agccttgacg
     2941 agggtgggat cggtcaagga gacgacggcg aggtcggcgc ccctgggata ggtgtagtag
     3001 atcacgccca catcccaggc gatgcggccg gtgttgaact tgatgccggc ataccagtcc
     3061 atttcgatgt tggcgatgtt ggtgccgtcg ttggcgcccc aatcgaggtt ggaggcccac
     3121 acgccggcgt agaacatgcc gcaggtcgca tcgaagccac cttgcaccgc cggcccctcg
     3181 gaggtctggg aaatgccgcg aaagacgtac tccgtggcga gcccgatgtt ggcggagagc
     3241 ttgcagcgct cttgcggtgg tggcggcggc gcatccttga tgctgccgcg ggccatgcca
     3301 tcggcgtggg ccgttccagc cagaagcgcg agcgcgatcg ccgccgcggt tggggccaca
     3361 agtgccggac ttcttacgtt catggtagct tccctagcga ttcgttgtct cgccaatctg
     3421 gccactttgg cctttggcgc aacggaaaat gccgccggcg cgcacggtcg caacggggac
     3481 gtgccacgtg cgcaacaggc ggcgcggcat tgggattttt tagtccggcg ctgcctattt
     3541 atcgggcgca tgcacggtgt ttttgtgcct gttggtgagg cagccccgca ccaggcgcga
     3601 cggccggctt ttgccgcccg cgccccgatg cgctaagaac cggataaccc gggccctgct
     3661 ggcgggccga tcgcgggctc acgggaggat gtggtggcag acgagacccc cccgaccgtt
     3721 ctcatcacgg gcgcagccca acgcatcggt cgcgcgctcg ccggcgattt tgcccggcgc
     3781 ggctggcggg tggcgatcca ctgccgcgcc tcgcagcggc aggcccagga gctggcgcaa
     3841 gagctcggcc gcagcggcac cgccagcgtt gtattgcctg cggatctggc ggatgccggc
     3901 gcggtcgccg ggctgatcgc ggaatgcagc cgccgcctgg gaccgcccac gtgtctcatc
     3961 aacaacgccg ccatgttcgc tcccgacgcc atcggcgagc tcgacggggc tttgtgggat
     4021 acgcagctcg ccgtcaattt gaaggctccg gtgttcctgg cggaagcctt cgccaaagag
     4081 ctgccgcccg gcgtggcggg caacgtcatc aacatcatcg atcaaggcgt gctgaagccg
     4141 accccccagt tcttttccta cacggtgtcg aaggctgcgc tgtgggccgc aacgcgcacc
     4201 ttggcccagg cgctcgcccc gcgcattcgc gtcaatgcca tcgggccggg cccggtgctc
     4261 aaaagcgcgc atcagaccga ggcccagttc cgcgccgaat atgaggccac gctgctcgaa
     4321 cggagcaccc gccccgagga aatcgccgcc gccatccgct tcattctcga ggccccagcg
     4381 ctgacgggac agatgctggc actcgatggc ggccagcacc tcgcctggca gacgcccgac
     4441 gtgactttgg gcgacaggtg acggctatgg cgacagcccc accgacgacc gctcccgaag
     4501 ccgccaagcc ggcgcgtccg agcggcagcc gccgcgtctt cgtgcgggat ttcgagatcg
     4561 tcgccagcgt cggcgttttc gaacacgaaa agcgctacga gcagcgcatt ctgctgtcgg
     4621 ccgatttggc ggtccgcgat gactatgacg gggtttccga tcggctggag gacgtcctcg
     4681 attacagcac gctggtcgag ggcattgccc ttttggtgca gcaggagcac gtcaatctgc
     4741 tcgagaccct ggccgagcgc atcgcccggc actgcctggc tgatcggcgc gtcgaaagcg
     4801 tcaggatacg gatcgagaaa ccggacgcgc tgccgatgtg ccgctccgtc ggtatcgaga
     4861 tcgagcgcgg ccgtgcctga cgcacgctga agcgtgctgc gcgcgagccc gcccaatcgc
     4921 cccatttgcg cccaaaagtg tagtgcacat tgaagcttac ggttcgctgc gattcgaagg
     4981 caaagccggc aacggcatgg ggaatcggcg cgaaaagcgt gagacgctcg ggggtcctgt
     5041 ggggggaact gccgggcgtg ggatggggac atcgggggat ggggacttgg ggtatggggg
     5101 cttggggtat gggggattga gtatgacggc caagtgggag ggtcgagcga ccgcaacagc
     5161 acacctcgtg cggggacgct aaccggccct taacgttaag ggccttatca acagaattga
     5221 aaatccgcgt gcgcctttaa gccgggggca caagggtgaa cttaggtggg tgttccatgc
     5281 cacgccgcgt cggagcgttt ctcctctttg ccttgcttgc tgccccttgt ccgctcgctg
     5341 ccgccgacct gcccgcgatc aaaaccaatc gcagcaatgc cgtgccggcc tgcgtcaccc
     5401 ccggtcgcct ccttgctttc ctggaaagcc gcaacccgca gcttgatccg cgcttccgca
     5461 gcatcgccgg cgactacatg ttgctcggcg acaggctggg ggtacgctgg gactatgcct
     5521 tttaccagat gctgctcgag accggctcgc tcagctttcg cgacggcaac cgttccggcg
     5581 atgtgaaagc gcagcagaac aactttgccg gtctcggcgc caccggccgc ggcgaacacg
     5641 gcgagagttt caaggacatc gccaccggcg tgcgcgcgca tctcgagcac ctcctgcttt
     5701 acgccggtga acgggtggaa aatcccgtcg ccgagcgcac gcgcaaggtc caggaatggg
     5761 acgtgctgac ggactggcac aagggcttca cgcggcccgt gaccttcggc gacctcgccg
     5821 ccaaatgggc ccccggcacg cgctcctacc gccgcatgat cgaaggcatc gccgagcgct
     5881 tcgaggaatt ttgtgcccgg cccgacccca agcccgagct gatgcaggag gcgcgggcgc
     5941 acaaagacaa gcgcgagagc aagccgagcg aggcgaagcc cgaacgggcc ggtggcaacg
     6001 aattggcgca gcgagcgatc gaggccggca aggccgagag caacgagaag cgtctgggcc
     6061 tcggtgtgca ggccaatgcc gccgcggcga cgtcggccac cgtgcccttg cgcatgctca
     6121 atcagccggc cgcagagccc ggcaaggagg ccgggccggc ggcggccatc gccgccgacg
     6181 atggcggtgg agctaagctc gagcgcgacc ggctggccgg cgccatcacc gcgagtacgg
     6241 ccaagcctct agcgccgggc aaaaccgaca agtcaggcgg cgccgacaag tcgctgcaga
     6301 cggcctcgac ggcggcggcc gccaaggccg ccctgtcgcc ggcggcgggc cagaaatgcc
     6361 gggtctggac ggcgagttac ggcgggcaaa aggccatgat catcaagtcc cagggcgaca
     6421 aggtggtcaa ctacaccgtg ctcgacgtca acgagggctc cgaggcgcgc gaggcggagg
     6481 ccttcatttc cgcctacgcc aaaaatggcg ccattgccgg ccagttcggc agtcaagcgc
     6541 aagcgttgga aaaggccttc gagctctgtc ccgaaggcta gcgcggcgcc cggccgcggg
     6601 ctggccgggg caaccggcca agcgccttcc tggtgccgca attggcgcat gagagcttta
     6661 gaggttgccc ggggtgccgc ccgggcgccg gcgttgccct gcccggcgcg cccccttaat
     6721 cgccgagggg cgcggtgctg cctgcggcgc cgaaagcggc tatagctcgc cggggcgagg
     6781 gtattcggct ccggcctcca gcacacgaca cgaacggacg cgagttgcgc gatgttgaaa
     6841 ctcttcgccc gcctgccctt gttgggcttg atcctgatcg cggcgcaggc cttgcccgcg
     6901 gccggcgagc aaaatcgggc cgccgtaccc aacaatccca actgccgtaa caccggcagc
     6961 tttgacgcct ggctcgatga attccgtcag gaggcacgcc gcaataacat ctcgagcgcc
     7021 accatcgccg cggcgctcgg cggcatgacg ctcgatcccg gcatcatcca gcgcgaccgt
     7081 cggcagggct tcttcgccca aaccttcacg gccttttccg gcaagctgat tacgccaaac
     7141 cgcatccaga acggtctcgg gcggctcaag cagcaccgcg acctgttggc ccgtatcgaa
     7201 aatcaatggg gcgttcccgg accggtgatc gtcgccttct ggggactgga aagcgacttc
     7261 ggctcgatga tgggaaattt gccggtgctg cgctcgctgg ccacgctcgc ctacgactgc
     7321 cggcgccccg acatgttccg cgccgagctg atggaagcct tgcgcatcat cgatcgcggc
     7381 gatttgcgcc cggagaacat gatcggctcc tgggccggcg agctcgggca gacccagttc
     7441 ctgcccgcgc actacatgca gcacgccgtc gactatgacg gcgacggtcg gcgcgacctg
     7501 ttgcagagcg tgcccgacgt gctcgcctcc acggccaact tcattgcctt tctcggctgg
     7561 cagcggggcc agccttggct ggacgaggtg cggctcacgc gcgatttgcc gtgggccgag
     7621 gccgacctcg caatccagca ccccgtcgag aagtgggcgg gctggggggt caaatccgtt
     7681 agcggccggc ccctgtccag caccgccgcc acggcctcgc tgctgttgcc gatgggacgg
     7741 cacgggcccg ccttcctcgc ctatccgaac ttccaggtct acctgaagtg gaatcaatcg
     7801 ctcaactatt cgatcaccgc tgcgcatctg gccgatcggc tggccggcgc cccagccatg
     7861 gacaagggca atggcacggt gcccgagatg agcctgcagc aggtcaagga cctgcagggc
     7921 ctgctcgcca agcgcggtta tttcagcggc gaggtcgacg gcaagatcgg cgcggcgacg
     7981 cgcgctgccg tcaaggccgc gcaattgaaa ctggggctgc ccgccgattc ctacccgacg
     8041 cccgaactgc tcgagcgcat gcaggcgatg cgctaatcct cgagctcagg gccgcccagc
     8101 cgaggtcagc ggggggcgca gcgccgattc catccaggcc gccgcctccg ggcatggcca
     8161 agcctgcgtg cgacccgtcg gccgatcgag ctggagggag gtgcatccct ccgacgccgc
     8221 ttcgccgggt ccggccgaag caccgaccat aaggccgccg acgagctcat aaaggctggc
     8281 gaaattccac agcgccgcgc cgagcacgag cccggcaaag gctgcgcgac gcggccccaa
     8341 caggcgcatg cgcctgtgcc agcgttgttt gctcggtcgc ttgcccacga tcactcctgg
     8401 cacccctggc acccctggca ctgcctggcc gctcgtgcgc ccgcggcagc cccggccctg
     8461 cgcacgggct aggcttagcg gccgcggtcc gcccgcaaca atgccgcccg cgccaccctt
     8521 aaagatattc gcgctttcca caggtagcgc gcgctccccc gaggacctgg ctcagcggcg
     8581 gcgatcctcg cgcgggcccg ggacgatctc ggccgggctc gacggcacac gcgcgcccga
     8641 acccgcgcgc accggcggca catacatgcg ctcagccgta cacagccggc gcgggttttc
     8701 gttgccaccg cagcggcgaa aggcgccacc ccgatcaaag cagacgcgca cctcgcgcag
     8761 ccgatgcccc gtgccgccgc agtccaccgc cagcatgtcc ggctcgagcc cggggttggc
     8821 cgctacgaac tcgcgcacga gctcgccggg cgagagcagc aaacgctcgt cgctcagacc
     8881 gacgaagcgc tcggggatgc gcaccttgtc gtagagctgg cgggccagcg cgaagtagcc
     8941 gtcgacgccc agaccgctgc aggtgccgtg cttgcggtac tcgttgaaga tcaacttgtc
     9001 gctcggcatg atgtcgagca tgcgattggc caccggcctg ggcacgtagc cgcggtcggg
     9061 gctgcggcaa tgttccggcc agccgcgctc gtattgcggc cacaggccgt gcagcacgaa
     9121 ggcataaggt cgcgcgccac cgggccggca ctgcgggtca tagcgcgtgt cgccgacgtc
     9181 gctgcaatag gtggggctcc aggacagcgc cagcacgtaa tagtcgaagg cgccggcgcg
     9241 atgttcgctc ccccacg
//
LOCUS       DASTMT010000646         1665 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_6460011, whole genome shotgun sequence.
ACCESSION   DASTMT010000646 DASTMT010000000
VERSION     DASTMT010000646.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 1665)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1665)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1665
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6460011"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..334
                     /locus_tag="VFR00_14890"
     CDS             <1..334
                     /locus_tag="VFR00_14890"
                     /inference="COORDINATES: protein motif:HMM:NF021604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HEU0160607.1"
                     /translation="AVLQKDDASPAFLLTVDLDARVLNVRPVAAGSTAGKSYQLWIAM
                     ENQGGVPQSLGLIETPGAVTQRPLARYDRASVERATFGISLEPAGGSPTGRPTGPAFH
                     ARLIKTAG"
     gene            558..1031
                     /locus_tag="VFR00_14895"
     CDS             558..1031
                     /locus_tag="VFR00_14895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018898244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HEU0160608.1"
                     /translation="MVGGAAMYPSRNIVQNALNSRDHTTLVAAVKAAGLVETLSGPGP
                     FTVFAPTNAAFAKLPAGTVDTLLQPENKGKLTSLLTYHVVPKKALRNAIASMIESDGG
                     KHEITTVGGGKLWAYLQDGNLLLRDAKGRSAKVTIANVMQSNGVIHVIDSVLLPN"
     gene            1118..1435
                     /locus_tag="VFR00_14900"
     CDS             1118..1435
                     /locus_tag="VFR00_14900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0160609.1"
                     /translation="MQIKFVAAAAANAVALGLGVGSLEAAPGMGASAPRGAEAASIER
                     VTYAWYGGCSHFRSHRYGWHQHCHWRHRHCYWGQRCWGGYPSWGYRRRWGQDHDGQHR
                     AWA"
BASE COUNT          295 a          558 c          554 g          258 t
ORIGIN      
        1 cgcggtattg caaaaggacg acgcctcgcc ggccttccta ctcaccgtcg atctcgacgc
       61 ccgcgtgctc aatgtgcggc cggtagccgc cgggtcgacc gcgggcaaga gctatcaact
      121 ctggatcgcc atggaaaacc agggcggggt gccgcagtcg ctcggcctga tcgaaacgcc
      181 gggcgccgtc acgcagcgcc cgcttgcgcg ctacgatcgc gccagcgtcg agcgggctac
      241 cttcggtatc agtctcgagc cggcgggcgg ctcgccgacc ggacgtccga ccggcccagc
      301 ctttcacgcc agactgatca agacggccgg ctgagccgag gctcacaaga aaacgtgaac
      361 cggtgagtgc cgcaactcgc ggccgaagcc tgcgtacctc cagcgctcgc acgcatgagc
      421 agaaacgaaa ctggaggttg tctcgcatga aagccagcaa gcaaaatcaa tccatcgccc
      481 ttggcgcctc gctgctcgcc ttgggtctga tcgtcggcct catcgagccg gcgcccgcgg
      541 cacccgagcg aacggtcatg gtcggaggcg cggcgatgta tccctcccga aacatcgtgc
      601 agaacgcgct gaactcgagg gatcacacca ccttggtcgc cgcggtcaaa gccgcagggc
      661 tcgtcgagac cctgtcgggt cccggtccct tcacggtgtt cgcgccaacc aatgcggcct
      721 ttgccaagct gcctgcgggg acggtggata cgctgctgca gcccgagaac aagggcaagc
      781 tgacctcgct cttgacctat cacgtggtgc ccaagaaggc gctgagaaat gccattgcca
      841 gcatgatcga gagcgacggc ggcaagcacg aaatcacgac cgtcggcggc ggcaaactct
      901 gggcgtacct gcaggatggc aatctgctgc tgagggacgc gaagggtcgc agcgctaagg
      961 tgacgattgc caacgtgatg cagtcgaacg gtgtcattca cgtcatcgac agcgtgttgc
     1021 tgcccaactg acgggcgctt ggcgcctcgg cggccagcac gctgatcctc gcgcgcaggg
     1081 tcgtcgcgcc gccgacattg caagcatgga gatctcaatg cagattaagt ttgttgcggc
     1141 cgcggcggca aacgctgtcg ccttgggcct cggcgttggc agcctcgaag cggcgcccgg
     1201 catgggtgca tccgcccccc gcggagccga ggccgcttcg atcgagcggg tgacctacgc
     1261 ctggtacggg ggttgctcgc atttccgcag ccatcgctat ggctggcacc agcattgcca
     1321 ttggcggcac cgccattgct attggggcca acgctgctgg ggcggctatc cctcttgggg
     1381 ctatcggcgt cgctggggcc aggaccacga cggccagcac cgcgcctggg cgtaagcccg
     1441 ccgggggccg ggcacccggg cgccttggat caccacctgg tcccggccac gacgcgcagg
     1501 ccgccattct agcaaaaagg gcaagggccg cagtggcaca agtgggaggc gcgatgatct
     1561 cgcgcccccc ggcaatggcg cttaccgggg gcgcccaacg ccgcgcgcgg ccacgtggcg
     1621 gttgacaggg cgcatcaggc cgaggttctc gcgcaaggtg cggcc
//
LOCUS       DASTMT010000647         2425 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_5800551, whole genome shotgun sequence.
ACCESSION   DASTMT010000647 DASTMT010000000
VERSION     DASTMT010000647.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2425)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2425)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2425
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5800551"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..374)
                     /locus_tag="VFR00_14905"
     CDS             complement(<1..374)
                     /locus_tag="VFR00_14905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015241777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEU0160610.1"
                     /translation="MTAKRKATVFGIAMRNFTKYPEMPDARALIDYGVRMEELGYESL
                     WAWDHILLGVDPNFPIHEALALLTAVAARTTSIKVGTGILVMPVRNPVILAKELATID
                     HISNGRLLVGAAVGWYKREFDS"
     gene            complement(412..2022)
                     /locus_tag="VFR00_14910"
     CDS             complement(412..2022)
                     /locus_tag="VFR00_14910"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEU0160611.1"
                     /translation="MATTVEVLAEAFKQAGTPFIVGHPGGESVELMEAARQLDMRFLL
                     MKQEVAGAMLAATWGEISGSPGVCLSTRGPGAANMVNGVAHALLDRAPLIAITDAYAR
                     PTFETGLRQRLDQQALYAPLVKWSTTIDARTVRQQLRRAMRTATGLPPGPVHFELPQS
                     ETTREAGQDLAEPPLRPHLVCPRPELSDLKGVLETIRRAKRPILLAGLGVFWAKAADA
                     FVRFAERLGAPVLTTSKCKGAIPEDHPLRAGCIIGGLIERNLVTQADLIITVGLDAVE
                     LQPKPWPYTLPVLALTQTPSLDALVPADPEVVGELGALLSALAEWGPERASWGEKAAR
                     DFRVQVNAALDIPSRGLSPQRAVEIARAVLPRDTIATCDAGASRLLVVQKWQAYAARE
                     FLTSNGLGSMGYAVPGALGARLAHPKRPIVAFSGDGGFLMAVAELQTARRENLPIIVL
                     VFDDQEIGLIRIKQEIKKMPRHGVKLGGIDWEKLGHAFGADAAVVEHEAALGDALSAA
                     LKSGCTTLIAARIDASGYVDQFNALREL"
     gene            2218..>2425
                     /locus_tag="VFR00_14915"
     CDS             2218..>2425
                     /locus_tag="VFR00_14915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011158524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoglycerate dehydrogenase"
                     /protein_id="HEU0160612.1"
                     /translation="MATNKKKVLLTESMSKSGRDLLTERGDIEIKGFPNTIAAAEFQA
                     LLSAEAPVNAVALGGTRFGQKEIDA"
BASE COUNT          422 a          795 c          814 g          394 t
ORIGIN      
        1 aaggaatcga actcgcgctt gtaccaaccg accgcggcgc caaccaacaa gcgcccgttg
       61 gagatgtgat cgatggtggc aagctccttg gcgagaatca cgggattgcg caccggcatc
      121 accagaatgc cggttccaac cttgatgctc gtggtgcgcg cggcaaccgc cgtcagtagc
      181 gccagggcct cgtggatcgg aaagttcgga tcgacgccga gcagaatgtg atcccacgcc
      241 cacagcgact catagcccaa ttcctccatg cgtacgccat agtcgatgag cgcgcgcgca
      301 tccggcatct ccggatactt ggtgaaattg cgcatggcga tgccgaacac ggttgctttg
      361 cgcttggcgg tcatggtggc tccttcttgg cgtgacgtgg gcctgcgcgc tttagagctc
      421 gcgcagggca ttgaactggt cgacgtagcc cgaggcatcg atgcgtgcgg caatcagcgt
      481 ggtgcacccg gatttgaggg cggccgagag cgcgtcgcca agtgctgctt cgtgctcgac
      541 gacggcggca tcggccccga aggcgtggcc cagcttttcc cagtcgatcc cgccgagttt
      601 gacgccgtgg cgcggcatct tcttgatctc ctgcttgatg cggatgagac cgatctcctg
      661 atcgtcgaac accaagacga tgatgggcag gttttcgcgc cgggcggtct gcagctcggc
      721 caccgccatc aggaagccac cgtcccccga gaaggccacg atcggtcgct tcgggtgggc
      781 gaggcgagcg ccgagcgcgc cggggacggc ataccccatt gatcccaagc cgttggaggt
      841 caggaactcg cgcgccgcgt aggcctgcca tttctgtacc accagcaggc ggctggcccc
      901 ggcatcgcag gtcgcgatgg tgtcgcgggg cagcacggcg cgggcaatct ccacggcgcg
      961 ctggggcgaa agcccgcggc tcgggatgtc gagggccgca ttgacttgga cgcgaaagtc
     1021 gcgcgccgcc ttctcgcccc aactcgcgcg ctccggcccc cactcggcca gcgccgagag
     1081 cagggcgccg agctcgccca cgacctccgg atcggcgggc accaaggcat cgaggctggg
     1141 ggtctgcgtc agcgccagga cgggtagagt ataaggccag ggtttgggct gcagctcgac
     1201 tgcatcaagc ccgacggtga tgatgaggtc ggcttgcgtg acaaggttgc gctcgatcaa
     1261 gccaccaatg atgcagccgg cacgcagggg atggtcttcc ggaatcgcgc ccttgcattt
     1321 ggaggttgtc agcacgggcg cgccaagacg ctcggcaaag cgcacgaagg cgtcggcggc
     1381 cttggcccaa aacacgccga ggccggcgag caggattggc cgcttggccc ggcgaattgt
     1441 ctcgagcacg cccttgaggt cgctcagctc gggacgggga cagacgagat ggggccgcag
     1501 cggcggctcc gccaaatcct ggccagcctc ccgggtcgtc tcactctgcg gcagttcgaa
     1561 atgcaccggt ccgggcggca ggccggtggc cgtacgcatg gcccgccgca gctgctggcg
     1621 caccgtgcgg gcatcgatcg tcgtcgacca tttgacgagc ggggcgtaga gcgcctgctg
     1681 gtcgaggcgc tgacgcaggc cagtctcgaa ggtcggtcgc gcgtaggcgt ccgtgatggc
     1741 gatcagcggc gcgcgatcga gcagcgcgtg cgccaccccg ttgaccatgt tggcggcgcc
     1801 gggcccgcgc gtggaaagac acacccccgg gctgccgctg atttcgcccc aggtggccgc
     1861 caacatcgcc cccgccacct cctgcttcat gagcaggaag cgcatgtcga gttgccgagc
     1921 ggcttccatc agttccaccg actcgccgcc agggtggccg acgatgaacg gcgtccccgc
     1981 ctgcttgaag gcttcggcaa gcacttcgac ggttgtggcc acaggcttcc tccgacgcaa
     2041 cattagcgcg cgccgcccaa ctctatacca ggcgcgccgt cccgggcacg tcccacctcg
     2101 cggccgccca gggtccgcag ccgcccacgg ccgctcaaca gcaatgctca aagagcgtgg
     2161 gcaaacgccg ctcccctgtg ccatcctggc ctcctcactt caggtcaggg gacgtaaatg
     2221 gcgaccaaca agaaaaaggt gttgctcacc gaatcgatgt cgaaaagcgg ccgggatctg
     2281 ctgacggagc gcggcgatat cgagatcaag ggttttccca ataccattgc ggcggcggag
     2341 ttccaggcgc tgctcagcgc cgaggctccc gtcaatgcgg ttgcgctggg gggaaccagg
     2401 ttcggtcaaa aggagatcga cgctg
//
LOCUS       DASTMT010000648         1679 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_5800827, whole genome shotgun sequence.
ACCESSION   DASTMT010000648 DASTMT010000000
VERSION     DASTMT010000648.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 1679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1679
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5800827"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1217
                     /locus_tag="VFR00_14920"
     CDS             <1..1217
                     /locus_tag="VFR00_14920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006020301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sorbosone dehydrogenase family protein"
                     /protein_id="HEU0160613.1"
                     /translation="PAPKRDWLPTVNWSVAREPWRDGQTPKAATALQVVAFARGLKHP
                     RWLHVLPNGDVLVAEAATEPEASWSPRAIIQDMVQRRSGAIVENANRISLLRDKDGDG
                     VAEERFVLLEGLRQPFGMALIGERLYVANTDSVVSFAYRTGDTRIAEKGEKLLDLPVG
                     HHWTRALLASRDGSKLYVTVGSGSNIAERGLELERGRATILEFDIASRRSRIFASGLR
                     NANGMAFEPTAGQLWTVVNERDELGDDLPPDYLTSVKDGGFYGWPYSYWGRHVDHRVK
                     PPRPDLVATAITPDYALGAHTASLGLAYYAGSALPAPFRDGMFVGQHGSWNRSQYSGY
                     KVIFVPFKEGRPAGEPIEVLTGFLKDDASGIAHGRPVGVAIDSQGALLVADDTGNAIW
                     RVSAARRQAAPK"
     gene            complement(1373..>1679)
                     /locus_tag="VFR00_14925"
     CDS             complement(1373..>1679)
                     /locus_tag="VFR00_14925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012334347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HEU0160614.1"
                     /translation="EELKAEFNAAIAPVDAVLAPVALTPALPLEAVDQSTTPALFTRW
                     GNFLDLCALAVPNGFTRTGLPLSLQIVCRGGDEALALRIGWAWQNATDWHERSPQLP"
BASE COUNT          286 a          555 c          564 g          274 t
ORIGIN      
        1 tgccggcgcc caagcgggac tggctgccaa ccgtcaattg gtccgtggcg cgggagcctt
       61 ggcgggatgg ccagacgcca aaggcggcca cagccttgca ggtggtggca ttcgctcggg
      121 ggctaaagca tccccgctgg ctgcacgtct tgcccaatgg cgatgttctc gtggccgagg
      181 cagccaccga gcccgaggca tcctggtctc ctcgcgccat catccaggac atggtacagc
      241 gccgctccgg ggccattgtg gagaatgcca accgcatctc cttgctgcgc gacaaggacg
      301 gcgatggcgt ggccgaagaa cgcttcgtgc tgctggaagg cttgcggcag cccttcggga
      361 tggcgctgat cggcgagcgc ctctacgtcg ccaatacgga cagcgtcgtc agctttgcct
      421 atcgcactgg cgacacgcgc attgccgaga agggggaaaa gctgcttgac ctgccggtcg
      481 gccaccactg gacgcgcgcc ctgctggcaa gccgtgacgg ctccaaactc tacgtcacgg
      541 tgggatcggg cagcaacatc gccgagcgag gtctcgagct cgagcgcgga cgcgccacca
      601 tccttgagtt cgacattgcc agccgccggt cacgcatctt tgcctctggt ctgcgcaatg
      661 ccaacgggat ggcattcgag ccgaccgcgg gtcagttgtg gacggtcgtc aacgagcgcg
      721 acgaactggg cgacgatcta cccccggact atctgacctc ggtcaaggac ggcggcttct
      781 acggctggcc ttacagctat tggggcagac acgtcgatca tcgcgttaag ccgccgcgac
      841 ccgacctggt ggcaacggcg atcacacccg attacgcgct cggcgcccat acggcatcgc
      901 tcgggctcgc ctactacgcc ggcagcgcgc tgccggcgcc gtttcgagat ggcatgttcg
      961 tcggccagca cggctcgtgg aaccgcagcc aatatagtgg ctacaaggtg atcttcgtgc
     1021 ccttcaagga gggccggccc gccggagaac cgatcgaggt tctgaccggc tttctcaagg
     1081 acgatgccag cggcattgcg catggtcgcc ccgtcggcgt ggccattgac agccaaggcg
     1141 cccttctcgt tgccgatgat acgggcaacg cgatctggcg cgtcagcgcc gcccggcggc
     1201 aggccgcgcc aaagtaagcg gtgagcaagc atgggcaagc cggcgcgcgt gacgcaggct
     1261 ttcccctatg gctatccgcg cgtctcgaag tctggcgatt aagccaccct gccgtggccg
     1321 cccgaggacg gaccggcggc ggggcgagcc gtgcgggcgc gcgcgctgcg gctcagggaa
     1381 gctgcggcga acgctcgtgc caatccgtgg cgttttgcca ggcccagccg atacgcagtg
     1441 ccaatgcctc atccccgccg cggcagacga tttgcagcga cagcggcaga cccgtccgcg
     1501 tgaacccatt gggaacggcc agcgcgcaaa gatcgaggaa gttgccccag cgcgtgaaga
     1561 gagccggcgt cgtgctttga tcgaccgcct cgagcggcag tgccggcgtg agggcgaccg
     1621 gagccagcac ggcgtcgacc ggcgcgattg ccgcgttgaa ttcggccttg agctcttcg
//
LOCUS       DASTMT010000649        18661 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_3890157, whole genome shotgun sequence.
ACCESSION   DASTMT010000649 DASTMT010000000
VERSION     DASTMT010000649.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 18661)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18661)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18661
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3890157"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..978)
                     /gene="ychF"
                     /locus_tag="VFR00_14930"
     CDS             complement(<1..978)
                     /gene="ychF"
                     /locus_tag="VFR00_14930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018648334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEU0160615.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTQTAAAEAANYPFCTIDPNVGE
                     VGVPDPRLERLAGIAKSAAAVPTRLTFVDIAGLVRGASKGEGLGNQFLSHIREVDAVA
                     YVLRCFEDPDVTHVEGRIDPVADAETVETELMLADLESLERRREPLAKKAKSGEKEAK
                     VQLALIEKALAEVAEGRPARHARVAPEEERAFRQLQLLTAKPVLYVCNVDEASIGGNS
                     RSQKVRERAAAEGAGAVVICAKIEAELAGLNAEDRQDYLQHLGLAEPGLNQLIREGYR
                     LLDLITFFTAGPKEARAWTVPAGTKAPRAAGAIHTDFERGFIRAETIAFD"
     gene            complement(985..1764)
                     /gene="pth"
                     /locus_tag="VFR00_14935"
     CDS             complement(985..1764)
                     /gene="pth"
                     /locus_tag="VFR00_14935"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008835596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEU0160616.1"
                     /translation="MKLFVGLGNPGTAYARHRHNIGAMAVDQIAARHDLGPWRKRFHG
                     LTSEGSIGGRRVVLLKPQTYMNDSGRSVQDAQRFFKLADADVLVFHDELDLAPGKVRV
                     KMGGGNAGHNGLRSITAHIGNEYGRVRLGIGHPGAKHLVSNYVLHDFAKADAAWLEPL
                     LEAIADAAGRLAAGDEQRFLADLNRAAAKSAPAKSGDGRPGERKGAGEPGAGEGDRER
                     GARKDARRPLAAVAGGERRAKSLGALADNLKRWLKGRGQTD"
     gene            complement(1810..2574)
                     /locus_tag="VFR00_14940"
     CDS             complement(1810..2574)
                     /locus_tag="VFR00_14940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011443037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HEU0160617.1"
                     /translation="MAEASEIKASARPRAGKGGARAVRREGRVPGVLYGDKAAPQNIA
                     LDFKDLQLAVLRGKFLSTVHDLSLDGGKCRVIPREVQLDPVKDTPLHVDFQRVGPGAR
                     IRVNVPVRFINEGLSPGLKRGGVLNIVRHEVEVTAPADAIPDFFEFNLEGLEIGRSVH
                     ISATKMPEGVRPTIINRDFTVATIAGHKIEEEATTTPDAGVAVEGAVPAEGAEPAAAA
                     AGGDAKAPAGKEAAAKAPAGKEAAKPAAAKPAAGKK"
     gene            complement(2766..3542)
                     /locus_tag="VFR00_14945"
     CDS             complement(2766..3542)
                     /locus_tag="VFR00_14945"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014495653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU0160618.1"
                     /translation="MAPAPALGLFRGEAAIVTGSASNIGRAIALALAGEGAAVRCVDL
                     DDARNEAVIAEILAAGGRAEAVTADLSSRDGWRAALPANGAAVSLFVHSASPPRREVD
                     GALNVSEETFDAMLNTNVRSGFLLARALGQRMREQALKGRMLFITSLHAETPRNIPHY
                     SAAKAGQTMLVKELARALGPAGIRVNALAPGAVPGGGFNAAAFDFSAKIPLGRLGRPE
                     DMADMALALLSDRFSGYVTGTTVVVDGGIALYNWIPLPTP"
     gene            complement(3549..4142)
                     /locus_tag="VFR00_14950"
     CDS             complement(3549..4142)
                     /locus_tag="VFR00_14950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013949497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor
                     SspB"
                     /protein_id="HEU0160619.1"
                     /translation="MTAEPHIDYEALAQVAMRSMVRMVLARVAKTGLPGEHHFYIAFD
                     TQAPGVSLSKRLREKYPKEMTVVLQHRFWDLGVSDERFEVKLTFDGIPERLSIPFAAI
                     KVFFDPSVPYGLQFEESDLAGEALGSSEVAGQEVAPGKLEKKPRAPRKPRSEKAPELP
                     RPDRPTPGRPQIVASKPDSGDASNENKVVSIDAFRKK"
     gene            complement(4182..4544)
                     /gene="ssrA"
                     /locus_tag="VFR00_14955"
     tmRNA           complement(4182..4544)
                     /gene="ssrA"
                     /locus_tag="VFR00_14955"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01849"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="alphaproteobacterial-type; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01849"
     gene            4611..5393
                     /locus_tag="VFR00_14960"
     CDS             4611..5393
                     /locus_tag="VFR00_14960"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HEU0160620.1"
                     /translation="MLETDLAAAAGGLLIGAPAYQARTPWRPGWALLAAALITLTGIL
                     AAVAFVGVAKLAHPADDAPAVHGHVALTTLGVWQAVTIALTLTAGTLFGGRWGDVLAL
                     RAPTAGPVVYLTAILAMLALQIVVSVVQYFFVSGDMFADLRPFVDVALGPNWGLALLV
                     VGLGAPLAEELLFRGFLLSALARTRAGFAGAALISTTLWTALHAGYSLIGILEVFTIG
                     LFFSWMLWRTGSLRVPIFCHALYNSLIVCMLRFVALPSAPIG"
     gene            5518..6312
                     /locus_tag="VFR00_14965"
     CDS             5518..6312
                     /locus_tag="VFR00_14965"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEU0160621.1"
                     /translation="MQAYLDLLRRVRRQGVRKTDRTGTGTLSVFGHQMRFDLNAGFPL
                     VSTKKLHLKSVIHELLWFLRGDTNTAYLKANGVRIWDEWADADGDLGPIYGAQWRSWP
                     ARDGRSIDQIAELVETLKTQPDSRRLIVTAWNPADLPDMALAPCHCLFQFYVAEGRLS
                     CQLYQRSADVFLGVPFNIASYALLTMMLAQVTGHALGEFVHSFGDAHLYLTHLEQADL
                     QLARAPRPLPTMHLNPAVASIFAFTYEDFRLTGYDPHPHIRAEVAV"
     gene            6309..6830
                     /locus_tag="VFR00_14970"
     CDS             6309..6830
                     /locus_tag="VFR00_14970"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013949493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEU0160622.1"
                     /translation="MKGQARLAFVVAVAENGVIGRDGQLPWRLPSDLKHFRARTLGKP
                     VIMGRKTFASIGKPLAGRDNIVLSRQPGFHPPGVTVLARLTDAIELGRRLAAERGVEE
                     VTIIGGAEIFRAALPLADRIYLTLVKAAPEGETRIGNFPGEGWQETTREPMRKGPGDQ
                     YAADFIVLDRKKS"
     gene            7040..8149
                     /gene="hflK"
                     /locus_tag="VFR00_14975"
     CDS             7040..8149
                     /gene="hflK"
                     /locus_tag="VFR00_14975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HEU0160623.1"
                     /translation="MPWSNQSGGGGGRRGGGGPWGQGPSGGGPHHQPDLEEILKRSQD
                     KLKQVMPGGSGLPGSLVFLIAVVAAAVLAFYAFTFRVNADELGIVMFFGKPTRAEPPG
                     LHFRLPYPIEEVRLPKVTQQRIIEVGMRSGTGTRGFGGGIRDVPEESLILTGDENIVD
                     VDFVVFWRIKDARDYLFNIQNPEVTVKEVAESAMREVIGQSPIQPILTEARQKTEQAV
                     QKLMQSLLDAYGAGVRVDQVQLQKVDPPTQVIDAFRDVQAARADMERLQNEAFAYANR
                     VVPEARGEAERILQSARAYKEQSVAEATGQTARFLKVYEEYKKAPDVTRKRMYLETME
                     RVLGGTDKIIVDSKGGQGVVPFLPLDQLQKRKEKE"
     gene            8159..9064
                     /locus_tag="VFR00_14980"
     CDS             8159..9064
                     /locus_tag="VFR00_14980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HEU0160624.1"
                     /translation="MLRALAASVIVLLGAVAAVAYFSFFIVHVNEQAIVLEFGKPVRI
                     INEPGLFWKIPVVQTVDYFDKRILDIDTAPQEVTASDQKRIVVDTFARYRITDPLLFY
                     QTIRDERNVRSRLGPITESALRRVLGAATFQDVVRDKREALMKQIANQVNEEGKEFGL
                     QVVDVRIKRADLPEQNSKNIYDRMRAERQREAAEFRAEGSAGANRIRANADREVTIIK
                     AEAMREGERTRGDGDAQRNAIFADAFNRDQDFFAFYRTMQAYEAGIKPGDTRMLLAPD
                     SEFFKYFNSPGPNSTPPQPGAQPKQ"
     gene            9061..9246
                     /locus_tag="VFR00_14985"
     CDS             9061..9246
                     /locus_tag="VFR00_14985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HEU0160625.1"
                     /translation="MSDLLVAIGLVMVIEGLLWAVAPQVGLRLLTAAQQMPDQALRAA
                     GAAAVAAGVILVWLVRG"
     gene            9329..10912
                     /locus_tag="VFR00_14990"
     CDS             9329..10912
                     /locus_tag="VFR00_14990"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008506156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HEU0160626.1"
                     /translation="MSRLVAAQTTFETSRRARPRAGGPRSYVSRGGLAAGLLLALLGP
                     ALAQMRNGPTSVAPVAEKLIDAVVNISTSQTMKGPEGVPLPRVPKGAPFEEFFEDFFN
                     RRGGRSPADRKVSSLGSGFVIDGKEGLVVTNNHVIDGADEIIVNFHDGTKLKVDKVLG
                     KDTKTDLALLKVTPKKALPAVAFGGSTKMRVGDWVMAIGNPFGLGGSVTVGIISAKQR
                     DINSGPYDDFLQTDAAINKGNSGGPLFNMDGEVIGVNTAIISPTGGSIGIGFAVPSDT
                     AVAVIDQLRQFGETRRGWLGVKIQSITDELADTLGVQDNTGALVASVTPESPAAKAGI
                     QDGDIILKFDGKDVTSMRGLPRIVAQTPIGKEVDVEILRKGQKQNLRVAVARLVEDED
                     AAKAAAPKEAPKGKGKSRGKEKDKDKDKDKDSGAKPGGSMIGLVLAPLTDELREKNGL
                     GKDVKGVIVLEVDPASPAAEKGVKAGDVIVEVAQDAVTSVDDVTKGIDKVKKAGRKAV
                     LLRLESGKGGLRFVAIPVE"
     gene            10988..11701
                     /locus_tag="VFR00_14995"
     CDS             10988..11701
                     /locus_tag="VFR00_14995"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HEU0160627.1"
                     /translation="MPERHGLIDTIAEQITPVHPDGYKFLALGAGVTLLCFLLWPPLG
                     WLAALATAWIAYFFRDPPRVTPLREGLIIAPGDGKICALERVRPPGELGLGDSERLRI
                     SIFLSVFDVHINRAPLAGRIARSVYVQGSFLNAALDKASELNERRATVIVAPDGTEIA
                     VVQIAGLIARRIVSFVREGESIGTGQRLGLIRFGSRVDLYLPPGREALVALGQRAIGG
                     ETVFADLKSPEPEREARVQ"
     gene            11733..12626
                     /locus_tag="VFR00_15000"
     CDS             11733..12626
                     /locus_tag="VFR00_15000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013949486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylcholine/phosphatidylserine synthase"
                     /protein_id="HEU0160628.1"
                     /translation="MDPFLPKLEDAERRRRGRLLFRHNKIPVRMLLPNFFTLLSLCAG
                     LTAIRMAIELRYEMAIALVVIAALLDGVDGRLARALKAQSKFGAELDSLADFVNFGVA
                     PAVIVFIWGLGTLRGLGWISVLVFALAMGLRLARFNAMIEVDKPKWQGNYFTGMPAPA
                     GAITVLLPLYLDGLGLLDVRMWPVPIACYTLLMAFLLVSTIPTFSGKLLGERIAREYV
                     LPIFVAAAALVALLVTYPYETLTLVTIVYLGVIPVSYQRYQQHLRQPSPEPATAAQGS
                     AAALTPEDTGVAGAPASETKH"
     gene            complement(12649..14091)
                     /locus_tag="VFR00_15005"
     CDS             complement(12649..14091)
                     /locus_tag="VFR00_15005"
                     /EC_number="2.7.7.13"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate
                     guanylyltransferase/mannose-6-phosphate isomerase"
                     /protein_id="HEU0160629.1"
                     /translation="MGQIYPVLLSGGAGTRLWPLSRAMYPKQFIRALAGEGPCLLAAT
                     LQRTAAEPDFASPIIVCNSDHRFLVEEEAALAGVKPRAIVLEPVARNTAPAIAAAALM
                     VAAEDPEGIVLVMPSDHVITDRANFLAAVRGAAEVAASGKLVLFGIKPSAPNTGYGYI
                     RRGAPLAGFDNAFKVDAFTEKPDAETAAQYLAAGNYAWNSGIFVLGVGTFLAELARLA
                     PALLEGVRAALEAASMDLGFLRLDAQAFQGVPNISIDYAVMERTAAAAVMPVDFGWSD
                     VGSWSSLWDLARRDGQGNAVMGDALLSDTTNCYVRSERGLVSTIGIKDLIIVDTPDAV
                     LVADRAKAQDVGSLVAKLKAAKRKEHEQHLRNYRPWGFFATLNVGPRFQVKLLHIKPG
                     AKLSLQMHHHRSEHWVVVQGTAKVVIGDSERLVRENESVYIVATQWHRLENPGKTPLE
                     LIEVQIGSYLGEDDIVRADDIYHRAPEETK"
     gene            complement(14210..16114)
                     /locus_tag="VFR00_15010"
     CDS             complement(14210..16114)
                     /locus_tag="VFR00_15010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015596506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU0160630.1"
                     /translation="MSTTAALEPSARDRDRSIGRIVSVTGAKAIVLLDGCGDEGQRLQ
                     VSADKPEMGTLFAIDTPHTVVLSIVSALNVPVPAHREGEGELWIAELGLVGELVKRGN
                     GTVGFNRGVSIYPTLGDRVRVASRIELEQAFYDDKRHSVRVGCIRQDPSIAAMIRVDD
                     LLGKHFAILGTTGTGKSCTTALILRSILQRNPAAHVLLLDPHNEYASSFGEWGEVISP
                     RNMQLPDWLLTFEELIEVLIGNPQERKAEIEILQELIPLAKCRYGSGRGTAARELRRS
                     STDARYTVDTPVPYRMSDLLQVIDERMGKLENKRDLSPYRALKTRLETITLDPRYAFM
                     FGSLTIYDSMAQILGRMFRVPVNGKPITILELTGLPTEIVNVVVSVLCRMTFDFALWS
                     EGQVPITLVCEEAHRYVPANPSFGFEPCKRAIAKIAKEGRKYGASLCIVTQRPAEIDP
                     TILSQCNTVFALRMSNDRDQEIVKSAIADTGSGLLEFLSALGQREAMAFGDGVSLPVR
                     IKFDELPAHAMPKSSSARFSEMWQKSVGDEGFLDSIVDRWRNASAAAADAVQGTALFA
                     ESVNLLPHPAEAMLPAPPAFEERRTPARETPPERREFGTLRREPNAAGAQPTQAGTAV
                     RAIREKLHHR"
     gene            complement(16092..16979)
                     /locus_tag="VFR00_15015"
     CDS             complement(16092..16979)
                     /locus_tag="VFR00_15015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEU0160631.1"
                     /translation="MKDYSAVLAESVLRHRAWFAEHCARIETELAGKVKSEFIANMSH
                     ELRTPLNTIIGFAKLLGEQQKRQHQHGQIVEYADLIRDAATHLLAVINDILDISKLQS
                     GKYSVDSREVDVDEVLELALAALRPAALARGILIEGKLAHPLPSVRGDAGKLRQVFSN
                     LIGNAIKFTPAGGSVTVTASALADGGVSIRICDTGVGMTKAEVALALTPFGQVDAGLS
                     RWQEGAGLGLPIAKALVQLHGGTLEINSAASMGTEVLITLPSRHQVKGSKDGVEPRRP
                     VAAPQLPLRKDDHVDNGSP"
     gene            17383..18042
                     /locus_tag="VFR00_15020"
     CDS             17383..18042
                     /locus_tag="VFR00_15020"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011088064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEU0160632.1"
                     /translation="MSLRIGDTAPDFDAETTAGRIKFHDWIGSSWAVLFSHPKDFTPV
                     CTTELGYMAKIKPEFDRRDVKIIGISVDPIDRHAKWAEDIKETQGHAPNYPMIGDPDL
                     KVSKLYGMLPAETGGSSEGRTPADNQTVRNVFVVGPDKKIKLVLVYPMTTGRNFDEVL
                     RVIDSLQLTAKHKVATPVNWKHGEEVIIAGSVSDEDAKKQYPQGWKAPKPYIRIVPQP
                     R"
     gene            18335..>18661
                     /locus_tag="VFR00_15025"
     CDS             18335..>18661
                     /locus_tag="VFR00_15025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018859372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU0160633.1"
                     /translation="MIFRQLFDSVSSTYSYLIASRRGGEALIIDPVLEKVDRYLQLVG
                     ELDLKLVKAVDTHIHADHITGLGALRDRTHCITVMGENAKVDVVSMRVAEGDRITIEG
                     MALEVLY"
BASE COUNT         3152 a         6171 c         6184 g         3154 t
ORIGIN      
        1 gtcgaaggcg atggtttccg cgcggataaa gcccctctcg aaatccgtat ggatggcgcc
       61 tgcggccctc ggcgccttgg tgccggcggg cacggtccag gcgcgggcct ccttgggacc
      121 cgccgtaaaa aaggtgatga ggtcgagcag tcggtagcct tcgcggatca gctggttgag
      181 ccccggctcg gcgagaccca ggtgttgcag gtagtcctgc cggtcctcgg cattgaggcc
      241 agcgagctcg gcctcgatct tggcgcaaat caccaccgcg cctgcgccct ccgccgcggc
      301 gcgttcgcgc accttttgcg accgagaatt gccgccgatc gaggcctcat cgacattgca
      361 cacgtaaagg acgggcttgg ccgtcaacaa ctgcagctga cggaaggcgc gctcctcttc
      421 gggcgcgacg cgcgcgtggc gtgccggccg gccctcggca acctccgcca gggccttctc
      481 gatcaaggcg agttggacct tggcctcctt ctcacccgac ttggccttct tcgccagcgg
      541 ctcgcgccga cgctccaggc tctccagatc ggcgagcatc agttcggtct cgaccgtctc
      601 ggcatcagca acgggatcga tgcggccctc gacgtgcgtg acgtccgggt cctcgaaaca
      661 gcgcaacacg taggcgacgg catcgacctc gcgaatgtgc gaaagaaact ggttgcccaa
      721 gccctccccc ttcgatgccc cgcgcacgag accggcaata tcgacgaagg tcaggcgcgt
      781 cggcaccgcc gcggcggact tggcgatgcc ggcgaggcgc tccaggcgtg gatccggcac
      841 accgacctcc ccgacattgg gatcgattgt gcagaacgga taattggcgg cctccgctgc
      901 cgccgtctgc gtcagcgcgt tgaagagggt cgacttgccg acgttgggca ggccgacgat
      961 gccacacttg aagcccatgc caattcagtc cgtttgcccg cggcccttca gccagcgctt
     1021 gagattgtcg gcgagcgcgc caagcgactt ggcccggcgc tcgccgcccg cgacggcggc
     1081 gagcggccgc ctcgcatctt tgcgagcccc gcgctcccgg tcgccttcgc cggcaccggg
     1141 ctcgccggcg cctttgcgct cgccggggcg gccatcgccc gacttggctg gcgccgactt
     1201 ggcggcggcg cggttcagat cggcgagaaa gcgctgctcg tcgccggcgg ccaaccggcc
     1261 cgccgcatcg gcaatcgcct cgagcagggg ctcgagccag gccgcgtcgg ccttggcgaa
     1321 gtcgtgcagc acgtagttgc tcaccaggtg tttggcaccg gggtgaccaa tccctaagcg
     1381 cacgcggcca tactcgttgc cgatatgcgc ggtgatggag cgcagaccgt tgtggccggc
     1441 attgccgccg cccatcttca cgcgcacttt gcccggcgcc aggtcgagct cgtcgtggaa
     1501 taccagcacg tcggcatcgg caagcttgaa aaagcgctgg gcatcctgca ccgagcgccc
     1561 gctatcgttc atgtaggtct gtggcttgag cagcacgacg cggcgtccgc cgatgctgcc
     1621 ctcgctggtc agcccgtgga agcgttttct ccagggcccc agatcgtggc gcgcggcgat
     1681 ctgatcgacg gccatggcac cgatgttgtg gcgatggcgc gcgtaggccg tgcccggatt
     1741 gccgaggccg acaaacagct tcatgggacg gttgcccgct cagaggcgcg ccgcagccgc
     1801 ggcgcgggac tatttcttgc cggccgcagg cttggcggct gccggcttgg cggcctcctt
     1861 gcccgcgggg gccttggccg cggcctcctt gcctgcaggg gccttggcgt cgccgcccgc
     1921 cgcggcggct gcgggctccg cgccttccgc tgggactgcg ccctcgaccg ccaccccggc
     1981 atccggcgtc gtggtggctt cctcttcgat cttgtggccg gcgatggtgg ccaccgtgaa
     2041 atcgcgattg ataatggtcg gccgcacgcc ctccggcatc ttggtggcgg agatgtgaac
     2101 cgatcgcccg atctccagcc cctcgaggtt gaactcgaaa aagtccggga tcgcatcggc
     2161 gggcgcggtg acctccacct cgtggcgcac gatgttgagc acgccgccgc gcttcaatcc
     2221 gggcgacagc ccctcgttga tgaagcgcac cggcacgttg acgcgaatgc gcgcgccggg
     2281 cccgacgcgc tgaaaatcca catgcagcgg ggtgtccttg acgggatcga gctggacttc
     2341 gcgcgggatc acgcggcact tgccgccgtc gaggctcaga tcgtgcacgg tcgagaggaa
     2401 cttgccgcgc aagacggcga gctggagatc cttgaagtcg agggcgatgt tctgcggggc
     2461 agccttgtcg ccgtagagga cgccgggcac gcggccttca cgccggaccg cccgggctcc
     2521 gcccttgccc gcgcgcggac gcgcggacgc tttgatctca ctggcctcgg ccatggcgac
     2581 cactcctgat ggaaagcggg gccggcctcg aacgtgccgg taggccccaa ttggcgcgcg
     2641 ttatagcggc gtcggccgcg ccgcgcaacc atgcgcgggc ccgcccgagg ggaggccatc
     2701 cgccccggag cctacggcca ccctttgtta ggcaccgcgg gcggggcatg cctgccgcgc
     2761 aatcctcagg gcgtcggcag tgggatccag ttgtacagag caatgccgcc gtccaccacg
     2821 acggttgtcc ccgtgacata gccggaaaag cggtccgaca gcagcgccag cgccatatcc
     2881 gccatgtctt cgggcctgcc gagccggccc agtgggatct tggcggaaaa atcgaaggcg
     2941 gctgcattga agccgccgcc cggcaccgcc ccgggggcca gcgcattgac gcggatgccg
     3001 gccgggccca gcgcccgggc gagctccttc accagcatgg tctgcccggc cttggccgcc
     3061 gagtaatgcg gaatattgcg cggggtctcg gcgtgcaatg aggtgatgaa cagcatgcga
     3121 cccttgagtg cctgctcgcg catgcgctgc cccaaggcgc gcgccaacag aaagcccgag
     3181 cgcacgttgg tgttgagcat cgcatcgaac gtctcttcgc tgacgttgag cgcgccatcg
     3241 acttcccggc gcggcggcga ggcggagtgc acgaacaggc tgaccgcggc cccattggcg
     3301 ggcaaggccg cgcgccagcc atccctgctg ctgagatcgg ccgtcaccgc ctcggcgcgg
     3361 cccccggccg ccaggatctc ggcgatgaca gcctcgttgc gcgcgtcgtc cagatcgaca
     3421 cagcgcacgg cggcgccttc gccggccaag gcgagcgcaa tggcccgccc gatgttggag
     3481 gccgagccgg tgacgatggc cgcctcgccg cggaaaagac caagcgcggg ggctggcgcc
     3541 attatcgcct acttcttgcg gaaggcgtcg atgctgacga ccttgttctc gttgctggcg
     3601 tcgccggaat ccggcttgga ggccacaatc tgcggccgcc cgggcgtggg gcggtcgggg
     3661 cgcggcagct ccggcgcctt ctcggagcgt ggcttgcgcg gggcgcgcgg ctttttttcc
     3721 agcttgcccg gcgctacctc ctggcccgcc acctccgagg agccgagcgc ctcgccggca
     3781 aggtcggact cctcgaactg caggccgtag ggcacgctgg gatcgaagaa aactttgatg
     3841 gcagcaaagg ggatcgaaag gcgctcgggg atgccgtcga acgtcagctt gacctcgaag
     3901 cgctcgtcgc tgacgcccag atcccagaag cggtgctgca gcaccaccgt catctctttg
     3961 ggatattttt cgcgcagacg tttggagagc gacacgccgg gagcctgggt gtcgaaggca
     4021 atgtagaagt gatgctcgcc cggcaggccc gtcttggcca cccgcgccaa caccatgcgc
     4081 accatcgagc gcatggcgac ttgcgccagc gcttcgtagt cgatatgcgg ctctgccgtc
     4141 atgcaccaac aaaaggccac ggcgggccgt ggcgcaagaa tagtggaggg cttctgttgc
     4201 ccggtgccct ccgaaaccgc gcctgccgtt gctagacgtc agggcttgac gttcagacgt
     4261 ttcgcgccgc gttacgcagc gagagccgtc tcagcatagt tgtcattggc aactattctg
     4321 attgacccga taacggcggt atcatgccga gcgaaagcgc accctttaca ccctcgtcga
     4381 tcctagttcg cccccagcag atgccgccgc cggcggcggc atgtggtgga ggcgccgggt
     4441 actgcccccg ggtccgatag gcttattgcg atgaccgttt atcgccatag ccggacgaat
     4501 ccggcccctc taatatagga aaacttggcc ccaaaaaaaa gcttccctgt gcgcggcctg
     4561 ccagccaatg agatggcggg atcgcaacga gcccgttggc gcgaggaccc gtgttggaaa
     4621 cagacctagc cgccgctgcc ggcgggctct tgatcggtgc gccggcctat caggcacgca
     4681 cgccctggcg gccgggctgg gcgctcctcg ccgcggctct gattacgctg accggcatcc
     4741 tcgccgctgt cgccttcgtc ggcgtggcca aattggccca tcccgccgac gacgcccctg
     4801 ccgtgcacgg ccacgtggcc ctgacgacgc tgggggtgtg gcaagccgtc accattgccc
     4861 tgacgctgac ggcaggcacg ctcttcggcg ggcgctgggg cgacgtgctg gcgctgcggg
     4921 cccccaccgc cgggcccgtc gtttacctga ccgcgatcct cgccatgctg gccttgcaga
     4981 tagtggtgag cgttgttcag tactttttcg tgtcgggcga catgttcgcc gacctgcggc
     5041 cctttgtcga cgtcgccttg ggaccaaact ggggcctggc actgcttgtg gtcggcctcg
     5101 gggcgccgct ggcggaggaa ttgctgttcc gcggctttct gctctcggcg ctggcccgca
     5161 cgcgcgccgg ctttgccggc gcggcgctga tcagcacgac gctgtggacg gcgctgcatg
     5221 ccggctactc gctgatcggc atcctcgagg tgttcacgat cgggctcttt ttttcctgga
     5281 tgctctggcg cacgggcagc ctcagggtgc ccatcttctg ccacgcgctc tacaactcgc
     5341 tgatcgtgtg catgctccgt ttcgtggcgc tgccgagcgc gcccattggc taggccccac
     5401 tggctaggcc ccactggcta ggccccactg gctaggcccc actggctagg ccccactggc
     5461 taggccccgt tggctcggcc ccgttggctc ggccaggaat cgctctagag taagggaatg
     5521 caagcctatc tcgatctcct gcggcgcgtg cgcaggcaag gggtccgcaa gaccgatcgc
     5581 accggcaccg ggacgctcag cgtcttcggg caccagatgc gtttcgacct caacgccggc
     5641 ttccccttgg tcagcaccaa gaagctgcac ctgaagtcgg tcatccatga gctgttgtgg
     5701 tttctgcgcg gggacaccaa cacggcctac ctcaaggcca acggcgtcag aatttgggac
     5761 gagtgggccg atgccgacgg cgacctcggc ccgatctacg gcgcccagtg gcgctcgtgg
     5821 cccgcccggg acgggcgctc gatcgaccag atcgccgagc tggtcgagac gctgaagacg
     5881 caaccggaca gccgccggct gatcgtcaca gcctggaacc cggccgacct tccggacatg
     5941 gccttggcgc cctgccactg cctgttccaa ttctacgtgg ccgagggccg cctctcctgc
     6001 cagctttatc agcgctccgc cgacgtcttt ctcggcgtgc ccttcaacat cgcaagctac
     6061 gcgttgctga ccatgatgct ggcgcaggtg accggtcacg cgctcggcga gttcgtgcac
     6121 agcttcggcg atgcgcacct ctacctgacg cacctggagc aggccgacct gcagctggcg
     6181 cgcgcgccac ggccattgcc cacgatgcac ctcaatcccg ccgtcgcctc gatctttgct
     6241 ttcacctatg aggattttcg cctcaccggc tacgatccgc acccgcacat ccgcgccgag
     6301 gtggcggtgt gaaaggccaa gcccggctcg cctttgtcgt cgccgtcgcc gagaacggcg
     6361 tgatcggccg cgacggccag ctcccctggc ggctgccctc cgacctcaaa cattttcgcg
     6421 cccgcacact tggcaagccc gtcatcatgg ggcgcaagac cttcgcctcg atcggcaagc
     6481 cgctcgccgg gcgcgacaac attgtgctgt cgcgccagcc gggcttccat cccccgggcg
     6541 tcacggtcct ggcgcggctc accgatgcca tcgagctcgg ccgtcggctc gccgccgaac
     6601 gcggcgtcga ggaggtcacg atcatcggcg gggcggagat tttccgcgcc gccttaccct
     6661 tggccgatcg catctatctg acgctggtca aggcggctcc cgagggggaa acccggatcg
     6721 ggaatttccc cggggaaggc tggcaggaaa ccacacgcga gcccatgcgc aaggggcccg
     6781 gcgaccaata tgccgccgat ttcatcgtgc tcgatcgtaa gaaaagttaa tcttgccaag
     6841 cgttggctat aggagccccc aaaccccata tggcacaagg cgcgggccta tttgcgcgcg
     6901 gtgggagtgt gccctataag cgacgagcac gcggatttcc ttgcaacact gggcgacggc
     6961 agtgggcggt ttcggtcgaa gcgccgaaac gaggaggccc acccacaagc cctaggcgac
     7021 gacgggagcg gcaaattcga tgccttggag caaccagagc ggtgggggcg gcggacgacg
     7081 cggcggtggg ggaccgtggg gccagggccc ctccggcggc ggcccgcatc atcaacccga
     7141 cctcgaagag atcctcaaac gcagccagga caagctgaag caagtgatgc cggggggctc
     7201 ggggttgccg ggatcgctgg tattcctgat cgccgtggtg gcggcggccg tgctggcctt
     7261 ttatgccttc accttccgcg tcaacgccga tgaactcggc atcgtcatgt tcttcggcaa
     7321 gccgacgcgc gcggagccgc cgggtctgca cttccgcctt ccctacccga tcgaggaggt
     7381 gcgcctgccc aaggtgacgc aacagcgcat catcgaggtc ggcatgcgct cgggcacggg
     7441 aacgcgcggc tttggcggcg gcatccgcga cgttcccgag gagagcctga tattgacggg
     7501 cgacgagaac atcgtcgacg tcgacttcgt cgtgttctgg cgcatcaagg atgcgcgcga
     7561 ttacctcttc aatattcaaa accccgaggt gacggtcaag gaagtggccg aaagcgccat
     7621 gcgcgaggtc atcggtcagt cccccattca acccattctg acggaggcgc ggcagaagac
     7681 cgagcaggcc gtgcaaaagc tgatgcaaag cctgctcgat gcctatgggg cgggcgtgcg
     7741 cgtcgaccag gtccagctgc agaaggtcga cccgccaacc caggtcatcg atgccttccg
     7801 cgacgtgcag gccgcgcgcg cggatatgga gcgcctgcaa aacgaggcct ttgcctacgc
     7861 caaccgggtg gtgccggagg cgcgcggcga ggcggagcgg atcttgcaga gcgccagagc
     7921 ctacaaggag caatccgtgg ccgaggccac cgggcagacc gcgcgttttc tcaaggtcta
     7981 tgaggaatac aagaaggcgc ccgacgtcac gcgcaagcgc atgtacttgg agacgatgga
     8041 gcgcgtgtta ggcggcaccg acaagatcat cgtggacagc aagggtgggc agggcgtggt
     8101 gccatttttg cccctcgatc aactgcaaaa acgcaaagaa aaggaatagg gacgagcaat
     8161 gctgagagcc ctcgctgcca gcgtcatcgt gctcctcggc gccgtcgccg ccgtcgccta
     8221 cttctcgttc ttcatcgtgc acgtgaacga gcaggcgatc gtgctggaat tcggcaagcc
     8281 ggtgcgcatc atcaacgagc ccggattgtt ctggaagatt ccggtcgtgc agaccgtgga
     8341 ttatttcgac aagcgcatcc tcgacatcga taccgcgccg caggaggtga cggcgtcgga
     8401 ccaaaagcgc attgtcgtgg acaccttcgc gcgctatcgc atcaccgatc cgctgctgtt
     8461 ctatcaaacc attcgtgacg agcgcaacgt gcgctcccga ctgggtccga tcaccgagtc
     8521 ggcgctgcgc cgcgtgctcg gcgcggcgac gttccaggac gtggtgcgcg acaagcgcga
     8581 agccctcatg aagcagatcg ccaatcaggt gaacgaggag ggcaaggagt tcggcctgca
     8641 ggtggtggac gtgcgcatca agcgcgccga cctgccggag cagaattcca agaacatcta
     8701 cgatcgcatg cgcgccgagc gtcaacgcga ggcggccgag ttccgcgccg aaggctccgc
     8761 cggcgccaat cgcattcgcg ccaacgcgga tcgcgaagtg accatcatca aggcagaggc
     8821 gatgcgcgag ggcgagcgca cgcgcggcga cggcgatgcc cagcgcaacg ccatctttgc
     8881 cgatgccttc aaccgcgacc aggatttctt cgccttttac cggaccatgc aggcctacga
     8941 ggccggcatc aagcccgggg acacgcgcat gctgctggcg ccggatagcg agtttttcaa
     9001 gtacttcaac agccccggac caaattcgac cccgccacag cccggagctc aacccaagca
     9061 atgagcgatc ttctcgttgc catcgggttg gttatggtaa ttgagggtct gttgtgggcg
     9121 gttgctcccc aggtggggct acggctcttg acggcggccc agcagatgcc ggaccaggcg
     9181 ctgcgggcgg cgggcgcagc ggcggtggcg gccggcgtca tcctggtttg gctggtgcgc
     9241 ggctagcggg gctggcgctg gcgtgcgccg gcattgggga agactcgggc ggacagttgg
     9301 tgtcggcgac ggtgaggaga caatggccat gagccggctt gttgcagcac aaacaacctt
     9361 cgaaacgagc cggcgcgcga ggccgcgtgc tggcggcccg cgcagttatg tttcgcgcgg
     9421 tggcctcgcg gcgggcctgc tcttggcctt gctggggccg gccctggcgc agatgcgcaa
     9481 cggccccacc agcgtggcgc ccgtggccga aaagctgatc gacgcggtcg tcaacatttc
     9541 caccagccag accatgaagg ggccggaagg cgtgccgctg ccgcgcgtcc ccaagggcgc
     9601 ccccttcgag gaatttttcg aggatttctt caatcggcgg ggcggccgct caccggcgga
     9661 ccgcaaggtg tcctcgctgg gctcgggctt cgtcatcgac ggcaaagaag gcctggtcgt
     9721 caccaacaac cacgtgatcg acggcgccga cgaaatcatc gtcaatttcc acgacggcac
     9781 caagctcaag gtcgacaagg tgctcggcaa agataccaag accgacctgg cgctgctcaa
     9841 ggtgacgccg aagaaagcgc tgcccgccgt ggccttcggc ggctccacga agatgcgggt
     9901 cggcgattgg gtgatggcga tcggcaatcc gttcggcctc ggcggctccg tgacggtggg
     9961 gatcatctcg gccaagcagc gcgacatcaa ctcgggtccc tacgatgatt tcctgcaaac
    10021 cgatgcggca atcaacaagg gcaactcggg cggacctctc ttcaacatgg acggtgaggt
    10081 gatcggcgtg aataccgcca tcatctcccc gaccggcggc tcgatcggca tcggttttgc
    10141 cgttccctcc gatacggcgg tggcggtgat cgaccagctg cgccaattcg gcgagacgcg
    10201 ccgcggctgg ctcggcgtga agatccagtc gatcaccgac gagcttgccg acaccctggg
    10261 tgtgcaggac aacaccggag cgctcgtggc gagcgtcacc ccggagagcc cggccgccaa
    10321 ggccgggatc caggacggcg acatcatctt gaagttcgac ggcaaggacg taacgagcat
    10381 gcgcgggcta ccgcgcatcg tcgcgcaaac accgatcggc aaagaggtcg acgtcgaaat
    10441 cttgcgcaag ggccaaaagc agaacttgcg cgtggcggtc gcccgcctcg tcgaggacga
    10501 agacgccgcc aaggccgccg ccccgaagga agcgccgaaa ggcaagggca agagccgcgg
    10561 caaggagaag gacaaggaca aggataagga taaggacagc ggcgccaagc cgggaggctc
    10621 gatgatcggc ctggtgctcg cccccctgac cgacgagctg cgcgagaaga acggcctggg
    10681 caaggacgtc aaaggcgtga tcgtgctcga ggtcgacccc gccagtcccg ccgccgaaaa
    10741 gggcgtcaag gccggcgacg tcatcgtgga agtggcccag gatgccgtca cgtcggtcga
    10801 cgacgtcacc aagggtatcg acaaggtgaa gaaggcaggc cgcaaagccg tgctgttgcg
    10861 gctcgaaagc ggcaagggcg gcctgcggtt cgtcgccatc cccgtggaat aagcgagaac
    10921 cgccgagggc aaggcgcgcc agcgcacgaa cgcaagggaa tagtcctgca ggggaccggg
    10981 aggcagcgtg cccgaacgcc acggcctgat cgacaccatc gccgagcaaa tcacgcccgt
    11041 gcacccggat ggctataaat ttctagccct gggggccggc gtcactttgc tctgctttct
    11101 gctgtggccg ccgctcggct ggctcgctgc cctggcaacg gcctggatcg cctatttttt
    11161 tcgcgatccg ccgcgcgtca caccgctgcg cgagggcctc atcattgccc cgggcgacgg
    11221 caagatctgc gcgctcgagc gcgtgcgtcc gccgggcgaa ctgggtctcg gtgatagcga
    11281 acggctgcgg atctcgatct ttctgtcggt cttcgacgtg cacatcaatc gtgccccgct
    11341 cgcgggccgc atagcccgct cggtctacgt gcagggcagt tttctcaatg ccgcgctcga
    11401 caaggcaagc gagctcaacg agcgccgcgc cacggtgatc gtcgccccgg acggaaccga
    11461 aatcgccgtc gtgcagatcg ccggcctgat cgcgcgccgc atcgtcagct tcgtgcgcga
    11521 gggcgagagt atcggcaccg ggcaacgcct agggctgatc cggttcggct cgcgcgtgga
    11581 tctttatctg ccgccggggc gcgaagcgct ggtggcgctc ggtcagcgcg ccatcggtgg
    11641 ggagaccgtt ttcgccgatc tgaaatcgcc cgagcccgag cgcgaggcac gagtacagta
    11701 ggttttgcta ctgggaaagg taagcccaag ccatggaccc cttcctaccc aagctggaag
    11761 atgccgagcg ccggcgccgc ggccgcttgt tgttccggca caacaagatc cccgtgcgca
    11821 tgctgctgcc caattttttc accctgctca gcttgtgcgc cggcctcacc gccatccgca
    11881 tggccatcga attgcgctac gagatggcga tcgccctggt ggtcattgcc gccttgctcg
    11941 acggtgtcga tggacgcctg gcccgcgcgc tcaaggcgca gtccaagttc ggcgccgaac
    12001 tcgatagcct cgccgacttc gtcaatttcg gcgtcgcccc cgccgtgatc gtgttcatct
    12061 ggggccttgg cacgctgcgc gggctgggct ggatctccgt cctcgtgttc gccctggcca
    12121 tggggctgcg gcttgcccgc ttcaacgcca tgatcgaggt ggacaagccc aagtggcagg
    12181 gcaactactt caccggcatg ccggcgcccg ctggcgccat caccgtgctg cttccgctct
    12241 atctcgacgg cctgggcctg ctcgacgtgc gcatgtggcc ggtgccgatt gcctgctaca
    12301 ccctgctcat ggccttcttg ctggtttcga ccatcccaac cttttccggc aagctgctgg
    12361 gcgagcggat cgcccgcgaa tacgtgttgc ccatcttcgt ggcggccgcc gccctggtgg
    12421 cgctgctggt gacctacccc tacgagacgc tgaccctcgt caccatcgtc tacttgggcg
    12481 tcatcccggt cagctatcag cgctaccaac agcacctgcg ccagccctcc ccggagcccg
    12541 caacggccgc gcaaggcagc gccgccgcgc tcacgcctga ggacacaggg gtggccggcg
    12601 cgcccgccag tgagactaaa cactagccct ggcgccggca gcgccccact acttcgtctc
    12661 ttccggcgcg cggtgataga tgtcgtcggc gcgcacgata tcgtcctcgc cgagatagct
    12721 gccgatctgc acctcgatca gctcgagcgg ggtcttgccg gggttctcca ggcggtgcca
    12781 ctgggtcgcg acgatataga cggactcgtt ttcgcgcacg agccgctcgc tgtcgccgat
    12841 caccaccttg gccgttccct gcacgaccac ccagtgctcg gaacggtgat gatgcatctg
    12901 caatgacagc ttcgcccccg gcttgatatg cagcaacttc acctggaagc gcggaccgac
    12961 gttaagcgtg gcaaagaaac cccagggacg atagttgcgt aagtgctgct cgtgctcctt
    13021 gcgcttggcg gccttgagct tggcgacgag ggagcccacg tcctgcgcct tggcccgatc
    13081 cgccaccagc acggcatcgg gcgtatcgac gatgatcaga tccttgatgc cgatggtgga
    13141 gacgagcccg cgctcggagc gcacataaca attggtggta tcgctcagca gggcgtcgcc
    13201 catcaccgca ttgccctggc cgtcgcggcg cgccagatcc cacagggagg accaggagcc
    13261 gacatcgctc cagccaaaat cgaccggcat cacggcggcg gccgcggtcc gttccatcac
    13321 ggcataatcg atcgagatat tggggacacc ttgaaaggcc tgcgcatcga ggcgcaggaa
    13381 ccccagatcc atgctggcgg cctccagggc ggcgcgcacc ccctccagca aggccggcgc
    13441 caagcgcgcc agctcggcaa ggaaggtgcc gacacccaat acgaaaatcc cgctattcca
    13501 ggcgtagttg cccgccgcca gatactgcgc ggcggtctcc gcgtcgggtt tttcggtaaa
    13561 ggcatcgacc ttgaaagcgt tgtcgaagcc ggcgagcggc gcgccgcggc ggatgtagcc
    13621 ataacccgtg ttgggcgcgc ttggcttgat gccgaacagc accagtttgc cagaggcggc
    13681 gacctcggcg gcaccgcgca cggcggcgag gaaattggcg cggtcggtga tcacgtggtc
    13741 ggagggcatg accagcacaa tgccctcggg atcctcggcc gcgaccatca gggcggcggc
    13801 cgcaatcgcg ggcgcagtat tgcgggcaac gggctccagc acgatggcgc gcggcttcac
    13861 gccagcgagc gctgcctcct cttccaccag gaaacgatgg tcgctgttgc agacgatgat
    13921 cgggctggcg aaatccggct ccgctgccgt gcgctgcagc gtggccgcga gcaagcaggg
    13981 gccctcgccg gcgagcgcac gaatgaattg cttgggatac atggcgcgcg atagcggcca
    14041 caggcgggtc cccgcgccac cggacaaaag gactggataa atctggccca tggtagcgcc
    14101 ctcatccaat ttgcccgacc attagcagcg gggcagtggc aggggcaagg gcgccggcgc
    14161 ggcgccgcac aagcgggcac gcagcaaatc ttgttgccgc cgccagcgct cagcggtggt
    14221 gcagtttctc gcgaatggcg cgcacggcgg tgccggcttg ggtcggctgc gcgccggcag
    14281 cattgggctc gcggcgcagg gtgccgaatt cccgccgctc gggcggcgtt tcgcgcgccg
    14341 gggtgcgccg ttcttcgaac gccggcgggg cgggcagcat ggcttccgcg ggatgcggca
    14401 gcaggttgac gctttcggcg aacagggcgg tgccctgcac cgcatcggcg gccgcggcgg
    14461 aggcgttgcg ccagcggtcg acgatactgt cgagaaaacc ttcatcgccc accgacttct
    14521 gccacatctc gctgaagcgc gcgctggagc ttttcggcat ggcgtgcgcg ggcagttcgt
    14581 cgaacttgat gcgcacgggc aggctcaccc cgtcgccgaa ggccatggcc tcgcgctgcc
    14641 cgagggcgga aaggaactcg agcaggccgg agccggtgtc ggcgatcgcc gacttgacga
    14701 tttcctggtc gcggtcattc gacatgcgca gcgcaaacac ggtgttgcat tgcgagagga
    14761 tggtgggatc gatctcggcc ggccgctgcg tgacgatgca cagcgaggcc ccgtatttgc
    14821 gcccttcctt ggcgatcttg gcaatggccc gcttgcaggg ttcgaagccg aagctcggat
    14881 tggccggaac gtagcggtgg gcttcctcgc acaccagcgt gatgggcacc tggccctcgc
    14941 tccacagcgc aaaatcgaag gtcatgcggc aaaggacgga gaccaccaca ttgacgattt
    15001 cggtgggcag gccggtgagc tccaaaatgg tgatcggctt gccgttgacg ggaacgcgga
    15061 acatgcgccc gaggatctgt gccatgctgt cgtagatcgt caacgatccg aacataaacg
    15121 catagcgcgg atcgagggtg atcgtttcca gccgcgtttt cagggcccgg tagggtgaca
    15181 ggtcgcgttt gttctcgagc ttgcccatgc gctcgtcgat cacctgcagc aggtccgaca
    15241 tgcgataggg gacgggtgtg tccaccgtgt acctggcgtc ggtcgaggag cgccgcagct
    15301 cgcgcgccgc cgtaccgcga ccgctgccgt agcggcactt ggcgagcgga atcaactcct
    15361 gcaggatctc gatctccgcc ttgcgctcct gcggattgcc gatcagcacc tcgatcagtt
    15421 cctcgaaggt caagagccaa tcgggcagct gcatgttgcg aggactgatg acctcgcccc
    15481 actccccgaa cgagctggcg tattcgttgt gcggatcgag caaaagcacg tgcgcggccg
    15541 gattgcgctg cagaatggag cgcaagatca gggcggtagt gcaggacttg ccggtgccgg
    15601 tcgtccccag aatggcaaaa tgcttgccga gcagatcatc gacgcggatc atggcggcga
    15661 tggagggatc ctggcgaatg caaccgaccc gcacggaatg gcgcttatcg tcgtagaagg
    15721 cctgctccaa ctcgatccgc gaggcgacgc gaacgcgatc gccgagggtt gggtagatgg
    15781 agacgccgcg attgaagccg accgtgccgt tgccgcgctt gacgagctca cccaccaggc
    15841 ccagctcggc gatccacagc tcgccctcgc cctctcggtg cgccggcacg ggcacgttca
    15901 gggccgagac gatcgacaac accacggtgt ggggtgtgtc gatggcgaac aaggtgccca
    15961 tctcgggctt gtcggcgctg acctgcaggc gctggccttc atcgccgcac ccgtcgagca
    16021 ggacgatggc cttggcgccg gtgacggaga caatgcggcc gatcgaacgg tcgcggtccc
    16081 gcgcgctggg ctcaagggct gccgttgtcg acatggtcat ccttccttaa ggggagctgg
    16141 ggcgcggcaa ccgggcggcg gggctcgacg ccgtctttgc tgccctttac ctggtggcgc
    16201 gacggcagcg tgatcaggac ttcggtgccc atcgacgcgg cgctgttgat ctccagcgtg
    16261 cccccgtgca gctgcaccag tgccttggca atcggcaggc cgagaccggc gccctcctgc
    16321 cagcgcgaaa gcccggcatc gacttggccg aagggggtca gcgcgagggc gacctccgcc
    16381 ttggtcatgc cgacaccggt gtcgcagatg cggatgctga ccccgccgtc ggcgagcgcg
    16441 ctggccgtga cggtgacgct gccgccggcg ggcgtgaact tgatggcatt gccgatcagg
    16501 ttactgaaca cttgacgcag cttgccggca tcaccgcgca cgctgggcaa gggatgggcg
    16561 agcttgccct cgatcaggat gccgcgggcg agcgcggccg gccgcagtgc ggccagcgcc
    16621 agctcgagga cctcatcgac atcgacttcc cgggagtcga cgctgtattt gccgctctgc
    16681 agtttcgaaa tgtcgaggat atcgttaatg acggcgagca ggtgggttgc cgcatcccga
    16741 atgagatcgg catactcgac gatctggccg tgctggtgct ggcgtttttg ctgctcgccg
    16801 agcagcttgg caaagccgat gatggtgttg agcggcgtgc gcagctcatg gctcatattg
    16861 gcgatgaatt cggattttac cttgccggcg agctcggttt cgatacgcgc gcagtgttcg
    16921 gcaaaccagg cgcgatggcg caggacggac tcggccaaaa cggccgaata gtccttcatc
    16981 agcgtggcgc tcctgcccgg ttctacccta tcgcgcaaca tggccggacc gtaactttcc
    17041 ctactctggt cgggccttac cctaggtgcc agacgataaa gtgaggttaa tgccgggcgc
    17101 ggaggccggc cgtggctatt cgccttgcgt taagctttgg ctccccgtcg ccctcttgca
    17161 gccgccggcg ccgcgccccg gggcgggctg gggggtgact tgtagcagcc tttcaggtgc
    17221 cgcctggcgg atgcccttgc ggggcttgca gctgacgtgc gggggatgag gcgggagtgg
    17281 gcgggggtgt tgctgggcga tggtgcgggt gctaatgggc tttgtgcgct gccgccgcac
    17341 ccggcgcccg agtgaagcct agttctctgt aggagacctc agatgtcgct gcgaattgga
    17401 gataccgccc cggacttcga cgccgaaacc acggctggcc gcatcaagtt tcacgattgg
    17461 atcggcagct cttgggccgt gctgttttcc caccccaagg atttcacgcc ggtgtgcacc
    17521 accgagctcg ggtacatggc gaaaatcaaa cccgaattcg accgccgcga cgtgaagatc
    17581 atcggcatca gcgtcgatcc gatcgaccgg cacgccaagt gggccgagga catcaaggag
    17641 acgcaagggc acgccccgaa ctacccgatg atcggcgacc ccgacctcaa ggtctccaag
    17701 ttgtatggca tgttgccagc cgagaccggt ggctccagcg aagggcgcac ccccgccgac
    17761 aaccaaaccg tgcgcaacgt gttcgtcgtc ggaccggaca agaaaatcaa actcgtgctg
    17821 gtctacccca tgaccaccgg gcgcaacttc gatgaagtct tgcgggtcat cgattccttg
    17881 cagctgaccg ccaagcacaa ggtggcaacg ccggtcaact ggaagcacgg cgaggaggtg
    17941 atcatcgccg gttccgtttc cgacgaggac gccaagaagc agtacccgca gggctggaag
    18001 gcacccaagc cctatatccg catcgtgccc cagccgcgct aatcgccacc gagccaggcg
    18061 gcgcccctcg tgccgccgct ccttgcggag gtggatcgcg ccgcacgggc gccgggccgg
    18121 cagcaaccgg ggcgcgcccc cggggggcgc ccgcgcctgg gcgcggcctc tctttggccc
    18181 ttggcgtcaa ggcccagccc tgcggccgct ccctggccgc aggccgaccg aacgcccggc
    18241 gcggggcgag cggagttgcc cctcaaggga atatctgcct atcgcaggcg gagtgtgcta
    18301 gcataaagca tgcgcaatcc gcgcggaggt tgcgatgatt ttccgccaac tcttcgacag
    18361 cgtctccagc acctacagct atctcatcgc cagccggcgg ggcggcgagg cgctgatcat
    18421 cgatcccgtc ctggaaaagg tcgaccgcta cctgcagctt gtcggggaac tcgacctcaa
    18481 gctggtaaaa gccgtcgaca cgcatattca tgccgaccac atcaccggcc tcggcgccct
    18541 gcgcgaccgc acgcactgca tcacggtgat gggggagaac gccaaggtcg acgtcgtctc
    18601 catgcgggtc gccgagggtg acaggattac catcgagggc atggcgctcg aggtgctcta
    18661 c
//
LOCUS       DASTMT010000650         2584 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_5545097, whole genome shotgun sequence.
ACCESSION   DASTMT010000650 DASTMT010000000
VERSION     DASTMT010000650.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2584
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5545097"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            266..1243
                     /locus_tag="VFR00_15030"
     CDS             266..1243
                     /locus_tag="VFR00_15030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0160634.1"
                     /translation="MSFSCVGRLGAVAAMLLAFLLVEDVIADPPKPAGESVSKIETSA
                     IDRAPTAEPSAAPPLAAEKAVPAAAASDRSGFEMFLDRLMRAESGGRDTAANPRSTAL
                     GPYQFIKSTFLDLARRHFGVEVQELSEDEILRLRTDRTFARRCAETYSRENFVFLAEQ
                     GLQPTFGHLRLAFLVGPYAAARVLQAAPATPVAEVLGSAVVRANPFMARMSTSNLIAK
                     TARDVGESASEVDVPAHVRVAAAQPAPVASPRPVSRALRVRHACNPKLASCRKFAEMQ
                     TRVAKLGRQVAKVKQLSRRMARVAPSQASRALAANRRTTADTKRRGAGA"
     gene            complement(1218..1943)
                     /locus_tag="VFR00_15035"
     CDS             complement(1218..1943)
                     /locus_tag="VFR00_15035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002714204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0160635.1"
                     /translation="MPSALPPSAPLLEARSLDTYYGQRQALYGLSLRISKGETIGLMG
                     RNGMGKTTLVRTLVGLLRPRRGQVLLDGEDITALPTYAIAQRGVAYVPEGRGIFANLS
                     VAENLSIAEAGSAPGDWTLPRVLELFPRLAERLTHRGDQLSGGEQQMLAIGRALMMNP
                     RLLLLDEATEGLAPLLREEIWRTIRLIRATGMTTLLIDKTVAAVTAIADRTLILVKGE
                     IVFEGTPHELKARPELMHRHLGV"
     gene            complement(1943..>2584)
                     /locus_tag="VFR00_15040"
     CDS             complement(1943..>2584)
                     /locus_tag="VFR00_15040"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0160636.1"
                     /translation="VIGPNGAGKTTLVNMLSGMLAPSGGRILLEGRDVAGEPVWRMSR
                     LGIGRSFQKTNVFLPLSVLENVRLAVQATEAGLNPLKAGGRRGDLIERAETSLARVGL
                     LQQASRDAGALSHGEQRQLEIAMAIAGGAKVLLLDEPLAGMGVEESQRMVDLLRTLAR
                     EHAVLLIEHDMDVVFAVANWMTVMAEGSVLAEGTPEAIRANLSVQEAYLGASP"
BASE COUNT          439 a          857 c          850 g          438 t
ORIGIN      
        1 gccgaggccc ggcctgcggc cgcgccccag ccgtcaagtc tcccccaatc tgccactcga
       61 tcgccccatt ctgacaccta tctgaatagg cattcagact aggcggtcga gcccgagctt
      121 ccacttgtct cagggcggag ctcaatggtt gacggaaccg gcatactcgt gcaggtgcga
      181 tgtcgtgagg tgaggcgcaa aaaaaagctg gggtgtgcct caagaaggtt tgggaagcaa
      241 tcctcaaagc tagggaagca atcaggtgtc attctcatgc gtgggccgcc ttggcgcggt
      301 cgcggccatg ctgttggcct ttctgcttgt cgaggacgtc atcgccgatc caccgaaacc
      361 tgccggcgag agcgtttcca aaatcgaaac atccgccatc gatcgggctc ccacggcgga
      421 gccaagcgcg gcgccgccgc tagccgcgga gaaggcggtg cctgcggcag ccgcctccga
      481 tcgatcaggc ttcgagatgt ttctcgaccg cttgatgcgg gccgaatcgg gcgggcgcga
      541 tacggccgct aatccccgct ccacggcgct aggcccctac cagtttatca agtcgacctt
      601 cctggacctg gcgcgccgac atttcggggt tgaggtgcag gagctgagcg aggacgagat
      661 tttgcgcctg cgcacggatc gcacgttcgc gcgtcgctgc gcggagacct actcgcgcga
      721 aaactttgtt ttcctggccg agcaggggct gcagccgacg tttggccatt tgcgcctggc
      781 cttcctggtt gggccctacg ccgccgcccg cgtgctgcag gccgcgccgg cgacgccggt
      841 ggcggaggtt ttggggtcgg cggtggtccg tgccaatccc ttcatggcgc gcatgagcac
      901 cagcaatttg attgccaaga cagcgcgcga tgtcggcgaa agtgccagcg aagtcgacgt
      961 gccagcgcac gtgcgcgtgg ccgccgcaca gccggccccg gtcgcctcgc cacgcccggt
     1021 gtcgcgggcg ctgcgtgtgc ggcatgcctg caatcccaaa ttggcgagtt gccgcaagtt
     1081 tgccgagatg cagacgcgcg tcgccaagct cggccgccag gttgccaagg tcaagcagct
     1141 gagccgacgc atggccaggg tggcgccaag tcaggcctcg cgtgcgctcg ccgccaaccg
     1201 caggaccacg gccgatacta aacgccgagg tgccggtgca tgagctcagg gcgtgccttg
     1261 agctcgtgcg gggtgccctc gaagacaatc tcccccttga ccaaaatcaa ggtacggtcg
     1321 gcgatggccg tgacggcggc cacggtcttg tcgatcagga gcgtggtcat gccggtggca
     1381 cggatcaggc ggatggtgcg ccagatctct tcgcgcaaca gcggggcaag cccttcggtg
     1441 gcctcgtcga gaaggagcag gcgcgggttc atcatcaggg cacgaccgat cgccagcatt
     1501 tgctgctcgc caccggagag ctgatcgccg cgatgggtaa gccgctcggc aaggcggggg
     1561 aaaagctcaa gcacgcgcgg caacgtccaa tcccccgggg cgctccccgc ctcggcgatg
     1621 cttaaattct ccgcaacgct caggttggcg aagatacctc ggccctccgg cacataggcg
     1681 acgccgcgct gcgcaatcgc gtaggttggc agtgcggtga tatcctcgcc gtcgagcagc
     1741 acctggccgc ggcgtggtcg caacaagccc actagcgtgc gcaccagcgt ggtcttgccc
     1801 atgccgttgc ggcccatcag tccgatggtc tcgcctttgc tgatccttaa ggatagacca
     1861 tagagggcct ggcgctggcc gtagtaggtg tcgaggctgc gcgcctccag caggggcgcg
     1921 gatggaggca aagcggaggg catcagggcg aggctccgag ataggcttcc tgcacgctga
     1981 ggttggcgcg aatcgcctct ggcgtgccct cggccagcac cgatccctcg gccatgaccg
     2041 tcatccaatt ggctacggcg aacaccacgt ccatatcgtg ctcaatcaac agcacggcgt
     2101 gctcgcgggc gagtgtacgc aagaggtcca ccatgcgctg cgattcctcg acgcccatgc
     2161 cggccagcgg ctcgtctaac agcagcacct tggcgccgcc ggcgatcgcc atggcgatct
     2221 cgagctggcg ttgctcgcca tgcgacaggg ctcctgcgtc gcggctcgcc tgctgcaaaa
     2281 gcccaacgcg ggcaaggctc gtctcggcgc gctcgatcag gtccccccgg cgcccgcccg
     2341 ccttgagcgg attgaggccg gcctcggtcg cctgcacggc gagcctaaca ttttcaagca
     2401 cgctcagcgg cagaaagacg ttggtcttct ggaacgagcg gccgataccg agccggctca
     2461 tgcgccagac cggttccccg gcgacgtcgc gaccctcgag caggatgcgc ccgccgctcg
     2521 gcgccagcat gcccgaaagc atgttgacga gcgtcgtctt gcccgccccg ttggggccaa
     2581 tgac
//
LOCUS       DASTMT010000651         6258 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_6463475, whole genome shotgun sequence.
ACCESSION   DASTMT010000651 DASTMT010000000
VERSION     DASTMT010000651.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 6258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6258
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6463475"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..612)
                     /locus_tag="VFR00_15045"
     CDS             complement(<1..612)
                     /locus_tag="VFR00_15045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008969720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEU0160637.1"
                     /translation="MPASKSAVRRPLTAAPEGSELKSERAGAVRILTLARPEKRNSLS
                     EAMLGALAAAIAAADGDDSVRAVVLAAEGPVFSAGHDLKELTARRRDADGGRAYHAEI
                     MRRCSDMMRAIVRCRKPFIAAVQGPALAAGCQLVATCDLAVAASHATFATPGVNIGLF
                     CSTPMVALARNVPRKRAMEMLLLGEQLAADKAAEYGLVNRVVPA"
     gene            complement(627..1142)
                     /locus_tag="VFR00_15050"
     CDS             complement(627..1142)
                     /locus_tag="VFR00_15050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009337480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0160638.1"
                     /translation="MHQMIRLAIAAVLAVAFAYSPAPLAAQPVKQVKLTDKHVEGFIA
                     AQQDMAGVAEKMQGSSSDKPDPKIEAELESIAKKHGFQSFAEYDDVAGTISMVMAGID
                     PQTKAFTEPQIAIKKEIEEVSADKSIPEKEKKQMLDELNDALKSAAPIQFPSNIEIVT
                     KYYDKIDAVLQ"
     gene            1228..1695
                     /locus_tag="VFR00_15055"
     CDS             1228..1695
                     /locus_tag="VFR00_15055"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008196256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEU0160639.1"
                     /translation="MMGNDRVSSGLDAAQVEALINSHFPQIHVGGRAMLIEEAGVRRA
                     RVRLKKQQRHLRPGGTVSGPTMFTLADFSVYVAVIATLGEAGIEAVTTNMNINFLAKP
                     EPRDLIACVRLLRVGRRLAVGETELFSEGASDLVAHAIATYALPQVAPALGSQ"
     gene            1833..2297
                     /gene="rplM"
                     /locus_tag="VFR00_15060"
     CDS             1833..2297
                     /gene="rplM"
                     /locus_tag="VFR00_15060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEU0160640.1"
                     /translation="MPTYAAKPGELEKKWVLIDASGLIVGRLATIIATRLRGKHKPTY
                     TPHVDTGDNVIVINAEKVVFTGRKRDNKLYQWHTGYPGGLKTRTPRMIFDGKFPERIL
                     EKAVERMLKRGPLQRKLMRNLKVYKGAEHPHDAQKPEPLDVKALNSKNAARA"
     gene            2303..2782
                     /gene="rpsI"
                     /locus_tag="VFR00_15065"
     CDS             2303..2782
                     /gene="rpsI"
                     /locus_tag="VFR00_15065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEU0160641.1"
                     /translation="MAAETRTLEDLAALRGETPIAAPTYTQKLDTRGRAYATGKRKDA
                     VARVWLKPGAGRITVNSKDFAQYFARPVLQMLLKQPLQAANRLSQFDITATVSGGGLS
                     GQAGAVRHGISKALTYYEPELRAVLKKGGFLTRDSRVVERKKYGRVKARRSFQFSKR"
     gene            complement(2804..3034)
                     /locus_tag="VFR00_15070"
     CDS             complement(2804..3034)
                     /locus_tag="VFR00_15070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0160642.1"
                     /translation="MRTIVAWALGAVVVLGVSTTVEAKKPAKKPKQYAARQARSPADS
                     SQWHARDASQLPFGSTLWWDQMLRENRLTCCN"
     gene            complement(3119..3550)
                     /locus_tag="VFR00_15075"
     CDS             complement(3119..3550)
                     /locus_tag="VFR00_15075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0160643.1"
                     /translation="MSGSERLDPKCGPFANLWLTYSSGLDALAKGYEPVVLGLGRCQA
                     ELMTLALRRTQAWLDRPVRLSGCRTPQDAISEHLSFWQTAGAQYAESSQRVLAAVLST
                     SGAPLAAAGSTGADREARDYIAVAGRKEAAALPPIKDRRAA"
     gene            4362..5180
                     /locus_tag="VFR00_15080"
     CDS             4362..5180
                     /locus_tag="VFR00_15080"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEU0160644.1"
                     /translation="MKSILLPVDQSEQMSSALETAWQLAQKFDATIEGVALRPAFAEI
                     VAPDPIVAVTIPPADWNETDFVRNVRQTFDGFAASHPAEPGKATRFRWRGGSVIEDGA
                     LGSLGRVYDVTVLSRPGNRGARMTALEASLFDSGRPLLMAPPAPLPSFGQSVVIHWNA
                     STETARAMFMAMPLLRQAKRVMLLTLEGNVVPGPSPKDALGYLAANGVTAQEKTIPGR
                     TQRQGEVILAEATAQGADLLVKGAYTISRLRQMIFGGATSYILAAAEIPVFFAH"
     gene            complement(5212..>6258)
                     /locus_tag="VFR00_15085"
     CDS             complement(5212..>6258)
                     /locus_tag="VFR00_15085"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU0160645.1"
                     /translation="PFAGQLVDRLGARVTYGLGLTSLGAGYFLAGFATELWHYVIAVG
                     VLGGLGAAALGMVAASALLSRWFSHRIGAVMSVPYAAVGVGMLVLPPLTQLLLSRYDW
                     RATYQILGGFVLGLLPLALLFPLGRISAGSAHWQALRAAARASASGPWTVSSAVRTNA
                     FWGLFAVYLFTSLAAYSVTPHSVAYLIEQGLDPLAAAGAFGLAGALSVAGMLAVGWIS
                     DRFGRRRTATFSYLSTILGIVALSLVPLWPSLVLVYGFVLFFGLMQGVRGPIIVALVA
                     VLFPGGGVGAIYGTLSLAMGLGAALGAWGSGLLFGWTGSYLASFLLAIGAALAGMACF
                     WVIPSLRRERIAAP"
BASE COUNT         1132 a         2071 c         2045 g         1010 t
ORIGIN      
        1 ggccggcacg acgcgattga cgaggccgta ctcggcagcc ttgtcggcgg cgagctgctc
       61 gcccagaagc agcatctcca tggcccgctt gcggggcacg ttgcgcgcga gggcaaccat
      121 gggtgtcgag cagaagaggc cgatattgac ccccggcgtg gcgaaggtgg cgtggctggc
      181 ggccaccgcc aagtcgcagg tggcgaccag ctggcagccc gcggccagcg ccgggccctg
      241 gacggcggcg atgaacggtt tgcggcagcg tacgatggcg cgcatcatgt cagagcagcg
      301 ccgcataatc tcggcatggt aggcacgacc gccgtcggcg tcgcggcgcc gcgccgtcaa
      361 ctcctttaga tcgtggccgg cggagaacac cgggccttcc gccgccagca ccacggcgcg
      421 gacgctatca tcgccgtccg ccgccgcaat cgccgcagcc agtgcgccca gcatggcctc
      481 cgacagggaa ttgcgttttt ccggtcgtgc caacgtcagg atgcgcaccg cgccggcgcg
      541 ctcgcttttg agctcgctcc cctccggcgc cgcggtgagc gggcggcgca ccgccgattt
      601 cgacgctggc atcctcggct ccgctgctat tgcaggaccg cgtcaatctt atcgtagtac
      661 ttggtgacga tctcgatatt gctggggaac tgaatgggtg cggccgactt cagcgcatca
      721 ttcaattcat cgagcatctg cttcttctct ttttccggga tcgacttgtc ggcgctgacc
      781 tcctcgatct ccttcttgat ggcgatctgc ggctcggtga aggccttggt ctggggatcg
      841 ataccggcca tcaccatgga aatggtgccg gcgacgtcgt cgtactcggc gaaggactgg
      901 aagccatgct tcttggcgat cgattcgagc tcggcctcga ttttgggatc gggcttgtcg
      961 gagctgctgc cttgcatctt ctccgccacg ccggccatat cctgctgcgc cgcgatgaag
     1021 ccttcgacat gcttgtcggt cagcttgacc tgcttgacgg gctgcgcggc gagcggggct
     1081 ggcgagtagg cgaaagcgac ggccaaaacg gcagcgatcg ccaagcgaat catctgatgc
     1141 atgactatgt cctcgttgcg ctatcgggtg ggagcataga aaggtctggt aagggtgcca
     1201 aatgacaaaa gttggacgtg agggccgatg atgggcaacg accgcgtttc aagtggcctt
     1261 gatgctgcac aggtcgaggc actcatcaat agtcacttcc cgcaaattca tgtcggcggc
     1321 cgcgccatgc tgatcgagga ggcaggggtg cggcgcgcgc gcgtgcggct gaaaaaacaa
     1381 cagcgtcact tgcggcccgg tggcaccgtg tcgggcccga ccatgttcac gctggcggac
     1441 ttttctgttt acgtcgctgt cattgcgacg cttggcgaag ccggcatcga agccgtcacg
     1501 accaacatga atatcaattt cttagccaag cctgagccgc gcgatctcat cgcctgcgtg
     1561 cgtctcttgc gcgtcgggcg acgcctcgcc gtcggcgaga ccgaactgtt ctccgagggt
     1621 gcctccgacc tcgtggcgca cgccattgcc acctacgcgc taccccaggt cgcgccggcg
     1681 ctcggtagtc agtaggtaat attctacctc cagactatac cattgatttt cttgtattat
     1741 ttttcttatc tcgatgcgaa tcggcgttga cagggccggc ccggctcacg tacaagcggc
     1801 cgttctcgca aaccctgaga cggggagctt ccatgcctac ctatgccgcc aagcccggcg
     1861 agctggagaa gaagtgggtc ctgatcgacg ccagcggcct catcgtcggt cgcctggcga
     1921 ccatcattgc cactcgcctc aggggcaagc acaagccgac ctacacaccg cacgtcgata
     1981 ccggcgataa cgtgatcgtg atcaacgccg agaaggtcgt attcacgggg cgcaagcgcg
     2041 acaacaagct ctaccagtgg cacacgggct accccggcgg cctcaagacg cgcacgccgc
     2101 gcatgatctt cgacggcaag ttccctgagc gcatcctcga gaaggcggtc gagcgcatgc
     2161 tgaagcgcgg ccccctgcaa cgcaagttga tgcgcaatct gaaagtctac aagggagcgg
     2221 agcatcccca cgatgcgcaa aaacccgagc cgctcgacgt caaggcactg aactccaaga
     2281 atgcggcgag ggcgtagcac cgatggcagc tgaaaccagg acgctcgaag acttggctgc
     2341 gctcaggggc gagacgccca ttgccgcacc cacctacacc caaaagctcg atacgcgtgg
     2401 gcgcgcctat gccacgggca agcgcaagga tgcggtggcc cgtgtctggc taaagccggg
     2461 tgccggccgc atcaccgtca acagcaagga ctttgcccag tattttgccc gaccggtcct
     2521 gcaaatgctg ttgaagcagc cgctgcaggc ggccaaccgc ctgtcacaat tcgacatcac
     2581 ggcgaccgtg tccggtggcg gtctgtcggg ccaggcgggt gcggtgcgcc atgggatttc
     2641 caaggcgctc acctactatg agcccgagct cagagccgtc ctgaaaaaag gcgggtttct
     2701 cacgcgcgac agccgtgtcg tggagcgcaa gaagtatggc cgcgtgaaag cccggcgcag
     2761 cttccagttc tccaagcgct gaacgctcgc agctgcgcga gacttagttg cagcaggtca
     2821 atcggttttc gcgcagcatc tgatcccacc agagggtcga gccgaacggc agctgactgg
     2881 catcgcgcgc gtgccactgg ctgctatcgg cggggctgcg cgcctgtcgc gccgcatact
     2941 gcttgggttt tttcgcgggc tttttggcct cgaccgtcgt gctcacgccc aggaccacaa
     3001 ccgcgcccag cgcccacgcc acgatcgttc gcatggctca tgctcctcgc gcacatccat
     3061 cgcgttaaaa gcgctcgctc ggcgcgaacg tcaagcgcgg tagtgtctca gcggcgcctc
     3121 aggcggcgcg ccggtccttg atcgggggca acgccgcggc ctctttgcgc ccggcaacgg
     3181 caatatagtc gcgcgcctca cgatcggcac cggtgctgcc ggccgccgcc agcggcgcgc
     3241 cgctggtgct caagacggcg gcgagcacgc gctgggagct ctcggcgtac tgagcgcccg
     3301 cggtctgcca gaaacttaag tgctcgctga tggcgtcctg cggcgtccgg caccccgaga
     3361 gccgcactgg acggtcgagc caggcctgcg tgcggcgcag cgccagggtc atgagttcgg
     3421 cctggcagcg gccgaggccg agcacgacgg gctcatagcc cttggccaag gcgtcaagac
     3481 cgctcgaata agtgagccat agattggcaa agggtccgca tttcggatcg aggcgctcgc
     3541 tgccactcat ccccacactc caaggcttgc cacgctgctc caggcgaacg gcccggcgtc
     3601 tttttccggc gtcaggtgaa ggtatcgcca gctgcaaaac gccagaaacg ccggcggcaa
     3661 atcgtcgacc caagacgccg acgggccacg ccacgagatt atcgtcgctg ggttttgcca
     3721 gcaacgcaaa gcgccgaaag cggcggcgaa ttgcccagaa catgtgcata attttcaatc
     3781 gggagttgtg cgatggcgga ctcggctggt ttcctccctg ctctgggttg cgcggccgcc
     3841 ttggcgccgg gcccccgggc cgccggcagc gttggcctgc ggcggaaggt gcgcgcggcg
     3901 cggcgaggct ggcactgccg cgccgggagg ccttgccgcc gacgcgccga ggccaattgc
     3961 gatcgcgctg catcgcggct cgcggcgccc gcacgttcga gccaagccag tgtcctcagc
     4021 ctctgccgcg cgctaccgcg agaaagtggg ggaccgatcg ctggcctgat cgaccaggct
     4081 cccgcggatt gcggcgtggc gtgccgcgct tcgcggtcta tgtcgccgac gagcgcgcgc
     4141 ctgccgcggt gcggccacgc ggtgttgcgc gtgcgatgtt cggcagcgtg cgccgcgcaa
     4201 ggcgcgggcc cggcggtggg acgagggccg acacaggtgg ccgacacgcg ccgccgcgat
     4261 cggctcggcg gccgcgatat ctcccccttg cgggggcaac tgtgctatgc ttgcgctact
     4321 caaatcaaaa gaacttgtcc gtcaaagctg gatcgcccgc catgaagagc attcttttgc
     4381 ctgtggatca gagcgagcag atgtcgtcgg cactcgagac ggcctggcag ctcgcgcaaa
     4441 agttcgacgc caccatcgag ggagtggcgc tgcgcccggc attcgcggag atcgtggcgc
     4501 cggacccgat cgtcgccgtg accatcccgc ccgccgattg gaacgagacg gacttcgtgc
     4561 gcaatgtgcg tcagaccttc gatggctttg ccgccagcca cccggccgag ccgggcaagg
     4621 cgacgcgctt tcgctggcgc ggcggctcgg tcattgaaga cggtgcgctc ggcagtctgg
     4681 gacgggtcta tgacgtcacg gtcttgagtc gtcccggcaa tcggggtgcg cgcatgaccg
     4741 cgctcgaggc cagtttgttc gacagcggcc ggcccttgct gatggcgccc cccgcccccc
     4801 tgccgtcgtt cggtcagtcg gttgtcattc actggaatgc cagcaccgaa acggcgcgcg
     4861 ccatgttcat ggccatgcct ctgttgcgcc aggccaaacg cgtcatgctg ctgacgcttg
     4921 agggcaacgt cgtgcctggt cccagtccca aggacgccct gggctatctg gcggccaatg
     4981 gcgtcaccgc gcaggaaaag accattccgg ggcgcacgca acgccagggc gaggtgattt
     5041 tggccgaggc cacggcccaa ggcgccgacc tgctcgtcaa gggtgcctac accatcagcc
     5101 gcttgcggca gatgatattt ggcggggcca ccagctacat tttggcggca gccgaaattc
     5161 cggtgttttt cgcgcactga agcggccgtt gcggcccgcg cagcgtcgca actagggcgc
     5221 ggcaatgcgt tcgcgcctga ggctcgggat cacccaaaag caggccatgc ccgccagcgc
     5281 ggcgccgatc gccaacagga aagaggcaag gtagctgccc gtccagccga acagcagacc
     5341 ggagccccag gcaccgagcg ccgcgcccaa tcccatcgcc agcgacagcg tgccatagat
     5401 ggcgccgacg ccgccgccgg gaaacagcac ggcgacgagc gccacgatga ttgggccgcg
     5461 gaccccctgc atcaggccga aaaaaaggac gaagccgtaa accagcacca ggcttggcca
     5521 taagggcacc aggcttagcg cgacgatgcc aaggatggtg gagagatagg agaacgtcgc
     5581 cgtccgccgc cgcccgaagc ggtccgaaat ccagcccacg gccagcatgc cggcgacgga
     5641 caaggcgccg gccagaccga acgcccccgc cgccgccaag gggtcgaggc cctgctcgat
     5701 gagataggcc acggagtgcg gggtgacgct ataggcggcg agcgaggtga acaggtaaac
     5761 cgcaaacagg ccccaaaagg cattcgtgcg cacggccgag gagaccgtcc acgggccgct
     5821 ggcgctcgcc cgcgccgcgg ccctcagcgc ctgccaatgc gccgagccgg cgctgatacg
     5881 cccgagcgga aaaagcagcg ccaagggcag aagccctagc acgaaacccc ccaggatctg
     5941 ataggtggcg cgccaatcgt agcgggacag cagcagttgt gtgagcgggg gcagcaccaa
     6001 catgccgacg cccacggccg cgtaaggcac cgacatcact gcgccgatgc gatggctgaa
     6061 ccagcgacta agcaaggccg aggcggcgac catgccgagc gcggcggcgc caaggccgcc
     6121 caacacgccg acggcgatca cgtagtgcca cagctccgtg gcaaagccgg ccaggaagta
     6181 accggcgccc aggctggtca ggcccaagcc gtaggtcacc cgcgccccaa gccgatcgac
     6241 cagctggccg gcaaaggg
//
LOCUS       DASTMT010000652         4286 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_4899693, whole genome shotgun sequence.
ACCESSION   DASTMT010000652 DASTMT010000000
VERSION     DASTMT010000652.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4286
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4899693"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..663)
                     /locus_tag="VFR00_15090"
     CDS             complement(<1..663)
                     /locus_tag="VFR00_15090"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEU0160646.1"
                     /translation="MKVSLFYLPSLGSKADIEAGMAGTRADLYQQMLREVSEQCRLAD
                     ALGYDSVSFTEHHFHIEGFEVSNNPVLLDLYIAMQTKRIRVGQLGIVLPASNPIRVAE
                     DIAMLDHMTGGRACAGFARGYQRRWVDVMAQQMHGIHGALPHQHDEIDAANRAAFEES
                     FQIIKKCWTEEMLSFQGKFWKIPPGETPWPLETTAKWGKGVENGILTAVGVVPKPLQK
                     PHP"
     gene            complement(819..2732)
                     /locus_tag="VFR00_15095"
     CDS             complement(819..2732)
                     /locus_tag="VFR00_15095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEU0160647.1"
                     /translation="MLHINDLTYRIEGRLLFDKATAGIPSGHKVGLVGRNGSGKTTLL
                     RLIAGELAPDDGSLSVPRATRIGAVAQEAPGGEASLIDFVLAADRERAQLLAEAETAR
                     DPARIAEIHERLNDIAAHAAPARAHRILAGLGFDAAAQRRACGEFSGGWRMRVALAAM
                     LFTEPELLLLDEPTNYLDLEGTLWLEEYLAHYPSTVIIVSHDRELLNGSVTSILHLEQ
                     RKLTLYSGGYDTFEETRREKQRLDLKLKKKQDDERRRIEAFVIRFKAKASKATQAQSR
                     VKALARMQPIAELIEERVVPFAFPNPAKAMASPLIRLEGAAAGYHPEQPVLRGLDLSL
                     DADDRIGLLGANGNGKSTFAKLLAGRLAPIAGHRYGSDKIVVGYFAQHQLDDLPPQKT
                     PYEHMRELMGEATEAQKRARLGAWGFGVDKADTRAEHLSGGEKARLLLALATFHRPHL
                     LILDEPTNHLDVDAREALVRALNEYEGAVILISHDRHLMDACADRLWLVRDGTVTSYA
                     GDMARYRAECLAERRALDGGKANAPAPRRAKPSPQQARRQAAEQRAALAPLKQRVAKA
                     EAELEAASRAIAQIERALADPDMYRGDAARAQLLARERGKLIEVRAAAELAWLAASEA
                     YEAAVAETREGVP"
     gene            complement(2883..4085)
                     /locus_tag="VFR00_15100"
     CDS             complement(2883..4085)
                     /locus_tag="VFR00_15100"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEU0160648.1"
                     /translation="MSTPLLHRAEPEAVGLSSLRLRRIGEVLRREVAAERLPGAVLGI
                     MRAGRLAHLEAVGQRDRDKADPLKTDAIFSIASMTKAMTSTAIMQLVEEGRILLGDPV
                     ATYLPPLAALRVADGKGGTRAPKSAPTIQDLLRHSSGLTYQNRGTSYAHALYPGSSLA
                     AAERFSKDDTIAALARAPLLFDPGTDWEYGFSTDVLGFVVEAVTGKGLGAVLAERLWG
                     PLGMVDTGFALPVDKAHRYARALAKDPLSDAANPQIHHAGDKPQIWDSGGGGAVSTVT
                     DYLRFAEAMRAGGTLGSAQILGRATAALMTSDHLPASLNNRIADSMDPAAAGYGFGLG
                     FAVRRQSGIAAMAGSAGDYYWSGVYGTYFWVDPKEELSVVFMAAAPGLIRLRYRQLMR
                     NLVYQALT"
BASE COUNT          714 a         1422 c         1432 g          718 t
ORIGIN      
        1 cgggtgcggc ttttgcaaag gcttgggcac cacccccacc gcggtcaaaa tgccgttctc
       61 gacgcctttg ccccactttg ccgtggtctc gagcggccac ggggtttcgc cgggaggaat
      121 tttccagaac ttaccctgga aggacaacat ctcctcggtc cagcactttt tgatgatttg
      181 gaagctttcc tcgaacgctg cccgattggc ggcatcgatc tcatcgtgct ggtgcggcag
      241 tgcgccgtga atgccgtgca tctgctgtgc catgacatcg acccaacggc gctggtagcc
      301 gcgggcgaag ccggcacagg cgcgcccgcc cgtcatgtgg tcgagcatgg cgatgtcctc
      361 ggcgacgcga atgggattgg aggccggcag tacgatgcca agctgaccca cacgaatgcg
      421 cttcgtctgc atggcgatat agaggtcgag cagcacgggg ttgttggaga cctcgaaccc
      481 ctcgatatga aagtggtgct cggtaaagct caccgaatca tagccgagcg catcggccaa
      541 gcggcattgc tcggatactt cccgcagcat ttgctgatag aggtcggccc gtgtgccggc
      601 cattccggcc tcgatgtcgg ccttgctccc gagcgacggc agatagaaca gcgagacctt
      661 cattccttcc cccggcgtgc gccttgcctg ttggtactcc gttgccgaga gtgaagcctc
      721 ctcggcatga cggcaagagc ccgagagatc ggggcaccgg tcgggaaggg ctcttcgtgc
      781 gggtgccgat gggcgaaagc cttgggcggg cccacggctc agggcacgcc ttcccgcgtc
      841 tccgccacgg ccgcctcgta ggcttcgctg gcggcgagcc aggcaagctc cgccgccgcc
      901 cgcacctcga tcagtttgcc gcgctcgcgg gcgagcagct gagcgcgcgc cgcatcaccg
      961 cggtacatgt ccggatcggc cagcgcgcgc tcgatctggg cgattgcccg gctcgcggcc
     1021 tcgagctcgg cctcggcctt ggcgacgcgc tgcttgagag gggcaagggc tgcgcgctgc
     1081 tcggccgcct gccggcgcgc ctgttgcggg gagggcttgg cgcgccgggg cgccggcgca
     1141 ttggccttgc cgccatcgag cgcacgtcgt tccgccaggc actcggcgcg gtagcgcgcc
     1201 atatcacccg catagctcgt gaccgtgcca tcgcgcacga gccaaagccg atcggcgcag
     1261 gcgtccatca gatggcggtc gtggctgatc aggatcacgg cgccttcgta ctcattgagg
     1321 gcccgcacca gggcctcgcg ggcgtcgaca tcgagatgat tggtcggctc gtccaggatc
     1381 agaaggtggg gacgatgaaa ggtcgcaagc gccagcaaca ggcgtgcctt ttccccgccc
     1441 gacagatgct cggcgcgcgt gtccgccttg tctaccccga aaccccaagc ccccaaccgc
     1501 gcgcgctttt gcgcctcggt cgcctcaccc atcaattcgc gcatatgctc gtaaggcgtc
     1561 ttctggggcg gcaggtcgtc gagttggtgc tgggcgaaat agccaacaac gatcttgtcg
     1621 gaaccgtagc ggtgccccgc gattggcgcg agccggcccg ccaacagctt ggcgaacgtg
     1681 ctcttgccat tgccgttggc gcctaacagc ccgatgcggt cgtcagcatc gaggctgagg
     1741 tctaatcccc gaagcacggg ctgctcggga tgatagcccg ccgcggcgcc ctcgagccta
     1801 atcagcgggc tcgccatcgc tttggcagga ttggggaagg cgaacggcac cacgcgctct
     1861 tcgatcaact cggcgatcgg ctgcatccgg gcgagggcct tgacccggct ctgtgcctgg
     1921 gtggccttgg aggccttggc tttaaagcgg atgacgaaag cctcgatcct ccggcgctcg
     1981 tcgtcctgct tctttttgag cttgagatcg aggcgctgct tttcgcggcg ggtttcctcg
     2041 aaggtgtcgt agccccccga gtagagcgtg agcttgcgtt gctcgaggtg gaggatggaa
     2101 gtgaccgagc cgttcaacag ctcccgatcg tggctcacga tgatgacggt cgagggatag
     2161 tgggcaagat attcctcgag ccacaaggtg ccctcgaggt cgagatagtt ggtcggctcg
     2221 tccagcaaaa gcagctccgg ctcggtgaac agcatcgccg ccagggccac gcgcatgcgc
     2281 cagcccccgg aaaattcgcc acaggctcgc cgctgggccg ccgcatcgaa gccgagcccg
     2341 gccaggatgc gatgggcacg cgccggcgcc gcgtgcgcgg cgatatcgtt gagccgctcg
     2401 tggatctccg cgatgcgcgc agggtcgcgg gccgtctcgg cctccgccaa caactgcgcc
     2461 cgttcccggt cggcggccaa aacaaaatcg atgagactgg cttctccacc gggtgcctcc
     2521 tgggccaccg cgccgatgcg ggtggcgcgc ggcacgctga ggctgccgtc atcgggggca
     2581 agctctccgg cgattaagcg caaaagcgtg gtcttgcccg aaccgttgcg gccgaccaag
     2641 ccgaccttgt ggccggaggg aatgcccgcc gtggccttgt cgaacagaag tcggccctcg
     2701 atgcgatagg tgaggtcatt gatgtgcagc atgggggcta agccaatagc cgatgcgccg
     2761 cggcctgtca ttggctggca cgccggggcc cggatgccgg cgcccgccac aggcccgccc
     2821 caaggctggt gcgcaggccg gcgacacctc gcgctggtgc tggcgcgcgt caggtcacgg
     2881 cgtcaggtca gggcttgata caccaggttg cgcatgagct ggcgatagcg caggcggatc
     2941 aggcccggcg ccgccgccat aaacaccacg ctcaattcct ccttgggatc gacccagaaa
     3001 taagtgccat agacccccga ccaataataa tcgccggcac tgccggccat ggccgcgatc
     3061 ccgctctggc gtcgcacggc gaagcccaga ccgaagccgt agccagccgc cgccggatcc
     3121 atgctgtcgg cgatgcgatt gttgagcgag gctggcaggt gatccgacgt catcagcgcc
     3181 gccgtggcgc gccccaagat ctgcgccgaa ccgagcgtgc ctccggcgcg catcgcctcg
     3241 gcaaagcgta gatagtccgt gaccgtcgac accgctccgc cgccgccgct gtcccagatc
     3301 tgcggcttgt cgccggcatg gtgaatctgg ggattggccg catcgctgag cgggtctttg
     3361 gccagagcgc gcgcgtagcg atgggcttta tcgacgggca acgcaaagcc ggtgtccacc
     3421 atgccgagcg gcccccacag gcgctccgcc agcacggcac cgaggccctt tccggtcacg
     3481 gcctccacca cgaaacccaa gacgtcggtg gagaacccat actcccaatc ggttccagga
     3541 tcgaatagga gcggggcgcg cgcgagcgcg gcgatggtgt cgtccttgct gaacctctcg
     3601 gccgcggcta gcgaggatcc ggggtaaagc gcgtgcgcgt aactggtgcc ccgattctga
     3661 taggtcagcc ccgaggaatg gcgcaggaga tcttgaatag tcggcgcgct cttgggggcg
     3721 cgcgtaccgc ccttgccatc ggcgacgcgt agggctgcca gcggcggcag gtaggttgcc
     3781 acggggtcgc cgaggaggat gcgtccctcc tcgacgagct gcatgatggc cgtcgaggtc
     3841 atggccttcg tcatggaggc aatcgagaag atcgcatcgg tcttgagcgg gtcggccttg
     3901 tccctgtcgc gctgccccac cgcctccaga tgcgcgagcc tgccggcgcg catgatgccg
     3961 agcacggcgc ctggcagccg ctcggccgcc acctcgcgcc gcaaaacctc cccgatccgg
     4021 cgcaggcgca gggaggagag gccaaccgct tcgggctcgg cccggtgcag gaggggggtc
     4081 gacatggaaa acctcatggg cagcagatcg cgcgccgcac agtgcggcaa ggaccccagc
     4141 aagacaagca cgcgccgacg ccgtggggaa aggccggcat ggaacgaaaa aagaactttg
     4201 cctcgacagg cacgccgaat cgcggcatgt tcactttacg ttccgacgcg gcgccagtcg
     4261 cgaacgctgc cccgaacttc gccctc
//
LOCUS       DASTMT010000653         2267 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_5227096, whole genome shotgun sequence.
ACCESSION   DASTMT010000653 DASTMT010000000
VERSION     DASTMT010000653.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2267
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5227096"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..224
                     /locus_tag="VFR00_15105"
     CDS             <1..224
                     /locus_tag="VFR00_15105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008966063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEU0160649.1"
                     /translation="MRGHGDVVVGPSIQIAVYRAIYTDVNARLQAQAIALGGPVTYLD
                     KEEGEKTDATQQQVVLRPWELWKQQAMKP"
     gene            complement(315..563)
                     /locus_tag="VFR00_15110"
     CDS             complement(315..563)
                     /locus_tag="VFR00_15110"
                     /inference="COORDINATES: protein motif:HMM:NF018294.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1127 domain-containing protein"
                     /protein_id="HEU0160650.1"
                     /translation="MSLNTQTLNAGGANVAMVAHRLGELGKGLWRAYRRRRAERATIA
                     TLNGLGDNVLHDIGIDRSEIESVVRTRSKGRLQRFDGV"
     gene            complement(596..823)
                     /locus_tag="VFR00_15115"
     CDS             complement(596..823)
                     /locus_tag="VFR00_15115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0160651.1"
                     /translation="MIGIPNRRLSELAPEEVAALLRWAHQERARAVRAGVATTVGWLA
                     RLVREAGVAARLSEAPKPYPASAYARTRRCG"
     gene            945..1874
                     /gene="gcvA"
                     /locus_tag="VFR00_15120"
     CDS             945..1874
                     /gene="gcvA"
                     /locus_tag="VFR00_15120"
                     /inference="COORDINATES: protein motif:HMM:NF008352.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GcvA"
                     /protein_id="HEU0160652.1"
                     /translation="MPPLNALKAFEATARHLSFTKAAAELHVTPAALSHQIRGLEDML
                     GLKLFFRRARAIELTDAARLIYPGIQTGFESLRAAVERLEPSRQDRVLVVSATPGLTA
                     KWLAPRLYRFLAKHPDIDTRISAGSAYVNFATDGVDVGIRLSSGNHPELYVEKLSDEW
                     LLPLCSPRLLDGAHPLRSPKDLVHFTLIQVDLPGVVPTWSDWFRMAGIDGIDSTRGLR
                     LNVADHALDAASESAGVVLAYKVVASRDIGLGRLIVPFGPEIALPGRSYFFVCQKGHE
                     KRAPVKAFRDWVFAEIADTAAILRQATPGIAAA"
     gene            1938..>2267
                     /gene="fadH"
                     /locus_tag="VFR00_15125"
     CDS             1938..>2267
                     /gene="fadH"
                     /locus_tag="VFR00_15125"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: protein motif:HMM:NF012927.4"
                     /note="catalyzes the formation of trans-2- enoyl-CoA from
                     2,4-dienoyl-CoA; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HEU0160653.1"
                     /translation="MSAFPHLLQPLEFGFLTLPNRMLMGSMHTRIETLERPTERLARF
                     YAERARGGVALIVTGGFAPSEEGRLEPGGPIFKAASQIVEHTPITAAVHAHGGRIALQ
                     ILHAGRYA"
BASE COUNT          357 a          805 c          707 g          398 t
ORIGIN      
        1 tgatgcgtgg ccacggcgac gtcgtggtgg gacccagcat ccagatcgcg gtctatcgcg
       61 ccatctacac cgacgtaaac gcgcgcctgc aggcccaggc gatcgcgctc ggtggccccg
      121 tcacctatct cgacaaggag gagggggaga agaccgatgc cacccagcag caggtcgtgc
      181 tgcggccctg ggaactctgg aagcagcagg cgatgaagcc ctagcccagg acttcaccgc
      241 ctgtatcggc tgcattggcg ctgggcggcc gcggccgccc gcggcagcgg gccgaccggc
      301 ccgcggccac tccatcagac gccgtcgaag cgctgcaggc gtcccttcga gcgggtccgc
      361 accaccgatt cgatctcgct gcggtcgatg ccgatatcgt gcagaacgtt gtcgccaagc
      421 ccgttgaggg tggcgatggt ggcgcgctcc gctcggcgcc gccggtaggc ccgccatagc
      481 cctttgccga gttcccccag acggtgggcc accatggcga cattggcgcc gccggcattg
      541 agagtttgcg tgttcaagct catgaccatc tcctcaggtt cgaccgtgaa ttttttcagc
      601 cgcagcggcg cgtgcgcgca taggcgctcg ccggataggg cttgggagcc tcgctcaagc
      661 gggcggccac cccggcctcg cgcaccagcc gggcgagcca gccaaccgtc gtggcgaccc
      721 cggcacggac cgcccttgca cgctcctgat gggcccagcg cagcaacgct gccacctcct
      781 caggggcgag ctctgaaaga cgtctgttcg gtatcccaat catttgagct atcctcatgc
      841 catgtgcgtc ttggcacata gtaatacgtc caatatactt ggctcacaaa cgacgatatc
      901 tcgcttgaga gatgagagaa gctcaacgat ccatgcgccg ccccatgccc ccgctcaatg
      961 cgctcaaggc tttcgaggcc acggcgcggc acctgagttt caccaaggcc gccgcggagc
     1021 tgcacgtgac gccggcagcc ctcagccatc agattcgcgg gcttgaggac atgctgggcc
     1081 tgaagctgtt ttttcgccgc gcgcgtgcca tcgagctcac cgatgccgcg cgcctgatct
     1141 accccggcat ccaaacgggc ttcgaaagtc ttcgcgccgc cgtcgagcgg ctggaaccca
     1201 gccggcagga tcgcgtgctg gtggtcagcg cgacgccggg cctcaccgcc aagtggctcg
     1261 ccccgcggct ctaccgcttt ctggccaagc accccgatat cgacacgcgt atttcggcgg
     1321 gctcggccta cgtcaacttc gccaccgacg gcgtcgacgt cggcatccgc ctcagctcgg
     1381 gcaatcatcc cgagctctac gtcgaaaagc tctccgacga gtggctcttg cccttgtgca
     1441 gcccgcggtt gctcgacggc gcgcatccct tgcgttcgcc caaggacctc gtgcatttta
     1501 cgctgatcca ggttgatctt ccgggcgtgg tgcccacctg gtccgattgg ttccgaatgg
     1561 cgggcatcga tggcatcgac agcacgcgcg ggctgcgcct caatgttgcc gatcacgccc
     1621 tcgatgccgc cagcgaaagc gcgggcgtgg tgctcgccta caaggtggtc gcctcgcgtg
     1681 acatcggctt ggggcggctc atcgtgccat tcggacccga gattgcgctg ccggggcgct
     1741 cctatttctt cgtctgccaa aagggtcacg agaagcgggc gcccgtcaaa gctttccgcg
     1801 actgggtgtt tgccgagatc gccgataccg ctgccatcct gcgccaggcg acgccaggca
     1861 tcgccgcggc ctgaggccgg cgctcaatcg ccgcgcggct cggcacccct tgaacgcctc
     1921 gttgcacccg cattgcgatg agcgcctttc cgcacctgct gcagcccctc gagttcgggt
     1981 ttttgaccct gcccaaccgc atgctgatgg gctcgatgca tacccggatc gaaacgctcg
     2041 agcgtcccac cgagcgcctg gctcgcttct atgccgagcg cgccaggggt ggcgtcgccc
     2101 tgatcgtgac cggcggcttt gcgcccagcg aggagggccg gctcgagccc ggcggcccca
     2161 tcttcaaggc ggcctcccag atcgtggagc acaccccgat cacggcggcc gtgcatgcgc
     2221 atggcggccg cattgccctg cagatcctgc acgccggccg ctacgcc
//
LOCUS       DASTMT010000654         5149 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_3903808, whole genome shotgun sequence.
ACCESSION   DASTMT010000654 DASTMT010000000
VERSION     DASTMT010000654.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 5149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5149
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3903808"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..349
                     /locus_tag="VFR00_15130"
     CDS             <1..349
                     /locus_tag="VFR00_15130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HEU0160654.1"
                     /translation="TIPAEAAPPRRSAPARPSELPPPQKKLAEQLLAQGERNLSQGTV
                     AAARLFFRQAAEAGLALAAMRLGATYDPNELEKLKVQGLAADVTEARKWYERARELGA
                     PEAEQRLARLGRP"
     gene            382..1512
                     /locus_tag="VFR00_15135"
     CDS             382..1512
                     /locus_tag="VFR00_15135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008967248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HEU0160655.1"
                     /translation="MRRQISTGLLAALVLAAVPGEGSGQSAAPNAASATARRPAAATA
                     AAAAAGEESKAAQLNNWTVGVVGGFFEGTFIRLAVELAKALDDGEKLRVLPIVSYGGN
                     ENINDLLYLKGVDIAITYTDTFGLYKKSGQVRNIEQRINYISELLVGELYVFARPEIK
                     TLKDLDGKKVSLGIKGGSATTTGPILFERVGIRPEIIYISNTIALEKMKSGEVDAIVS
                     TGGKPNDLFVKLKPEPGFHFLAVDWSDRFADYYLPCPLSHEDYPLLIPAGETVPTLCM
                     QAVLAVYNFPRTSDRFRRVERFIEYYLERFDKLKQPSFQPKWREINLAANVPGWTRYW
                     VATEKLTALGKIAANEGARPRNEHEARFQEFLTWREKQGKKP"
     gene            1622..2446
                     /locus_tag="VFR00_15140"
     CDS             1622..2446
                     /locus_tag="VFR00_15140"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEU0160656.1"
                     /translation="MRASEALQSSTYHASDGVAYDRWLGRWAERLAEAVIDFAEFPGD
                     GDLLDVGCGTGALTFALAKRWPQRRVIGLDLAEPFIAHARARGGLSPAPSFALGDACA
                     LPYDDGQFAGAASQLVLMFIPKPERALREMLRVVRPGGTIAAALWDFRGGLTFQRLLW
                     DTAAALDPEARAMRNRLFSAPLVLPDGLRHLFQAGGLEDVERQSLTVRMDFADFDDYW
                     QPFLGGQGPVGGYFAKLDPELKVRIKAAVADAYCCGAPDGPRSLTATAWAVRGRVP"
     gene            2490..3797
                     /gene="oxlT"
                     /locus_tag="VFR00_15145"
     CDS             2490..3797
                     /gene="oxlT"
                     /locus_tag="VFR00_15145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015927933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxalate/formate MFS antiporter"
                     /protein_id="HEU0160657.1"
                     /translation="MVTAVAMDVSIPARGVRWTQLGLGILCMVMVANLQLGWTPFVDP
                     IDQKYHWGRYAIQWAFTIFVLTQTWLVPMHGYLADRFGPRVMISSAGLLVGAAWVLNA
                     YADTLLLLYLAAVIGGLGAGIVYGGAVGNALKWFPDRRGLAAGLTASGYGAGSALTVL
                     PIQSTIANWGYETAFFWFGLGQGIVVLLVGLLLRAPGASEVPNTAAAVPQAQRDFTLP
                     EVVRSPPFWVMYAMFVMVGVGGLMAQAQLGPMSKDFKIDSVPVTLLFLTLPALTFALS
                     LDRVLNGLTRPFFGWISDRIGREHTMFLAFLLEALAVYAMLTFAHDPLLFVILSGLVF
                     FAWGEIYALFPALCTDLYGRKFATANYGLLYTAKGMAALVIPLANLLPSGPGSWKLVF
                     MVAAGLDLLAALLALLVLKPLRKRHLAPTGAGEERPPAMPASP"
     gene            complement(3946..>5149)
                     /locus_tag="VFR00_15150"
     CDS             complement(3946..>5149)
                     /locus_tag="VFR00_15150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012046338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HEU0160658.1"
                     /translation="NAGRTGVGEVPGGEAIRRTIEDAKPLVLGRSLGDYNAILAAVHK
                     AFAARDRAGRGQQTFDLRTTIHAVTALEAALLDLLGQCLGVPVAALLGEGQQRQTVPA
                     LGYLFFIGDRRKTDLPYRAAPATAHAWDRLRDEEALTPAAIVRLAEAAQAKYGFSDFK
                     LKGGVLRGEEEIETVNALAERFPAARITLDPNGGWRLEDAIRLCQPLKGVLAYAEDPC
                     GAEGGFSGRETMAEFRRATGLKTATNMIATDWRELGHAMQLNAVDIPLADPHFWGLAG
                     SVRVAQLCAAWGLTWGSHSNNHFDVSLAMFTHVAAAAPGSITAIDTHWIWQDGQRLTL
                     EPLEIRGGALEVPARPGLGIRLDRQRVEDAHRLYKRQALGARDDAMAMQYLIPGWSFD
                     AKKPCLVR"
BASE COUNT          827 a         1739 c         1667 g          916 t
ORIGIN      
        1 gacgataccc gccgaggctg ccccgccgcg ccgcagcgcg ccggcccgcc cctcagagct
       61 cccaccaccc cagaaaaagc tggccgagca gctgctggcg cagggcgagc gcaatctgtc
      121 ccagggcacc gtcgccgccg cccgtctgtt ctttcgccag gcggccgagg ccggcctggc
      181 gcttgccgcc atgcggctcg gcgccaccta cgatcccaac gagctggaga aactcaaggt
      241 gcagggcctc gcggccgacg tgacggaggc ccgcaagtgg tacgagcggg cgcgtgagtt
      301 gggggcgccg gaggccgagc agcggctggc gaggcttggc agaccgtgag gcgagcggag
      361 agaggcaagg ggagtgcaac gatgcgtcgg cagatctcga ctggactatt ggccgccctg
      421 gtgctggcgg cggtccccgg cgagggcagc ggccaatcgg cggcgcccaa tgcggcaagt
      481 gcaacggcgc gccgcccagc cgcggccacg gccgccgccg ccgccgccgg cgaagaaagc
      541 aaagccgccc agctcaacaa ctggacggtc ggggtcgtcg gcggcttttt tgagggcacc
      601 ttcatacgcc tggccgtcga gctcgcaaag gcactcgacg atggcgaaaa gttgcgcgtg
      661 ttgccgatcg tcagttacgg gggaaacgag aatatcaacg accttctcta cctgaaaggg
      721 gtcgacatcg ccatcaccta caccgatacc tttggccttt ataaaaagtc cggccaggtc
      781 aggaatatcg agcagcgcat caattacatc tccgaattgc tggttggcga actttatgtc
      841 ttcgcccgcc cggaaatcaa aacgctcaaa gatctcgacg gcaaaaaggt cagtttgggt
      901 atcaagggcg gcagtgcgac caccaccggg ccgattttgt tcgagcgcgt cggtatccgc
      961 cctgagatta tttatatcag caataccatc gccttggaga aaatgaaaag cggcgaggtc
     1021 gatgccatcg tctctaccgg cggtaagccc aacgatctct ttgtcaaact gaaacccgag
     1081 ccgggctttc atttcctggc cgtagactgg agcgatcggt tcgccgacta ttacctgccc
     1141 tgcccgctga gccacgagga ttacccgctg ctgatcccgg cgggcgagac cgttccgacc
     1201 ttgtgcatgc aggccgtgct cgccgtttat aacttcccgc gcaccagcga tcgcttccgg
     1261 cgggtggagc gcttcatcga gtattacctc gagcgcttcg acaaactgaa acagccatcg
     1321 tttcagccga agtggcggga gatcaacctc gccgccaacg tgcccggctg gacgcgctac
     1381 tgggtggcca cggaaaagtt gacggcactg ggaaagatcg ccgccaacga aggcgcccgc
     1441 ccgcgcaatg aacacgaggc acgcttccag gaattcctga cctggcgcga gaagcaggga
     1501 aagaagccgt gagccgcggc gcggctgctc tcgccgctgc ggcggtcgcc gcggcaggga
     1561 tcagccaagt gccgatcacg accagcagca catcggaaag gcattagcaa cgtgaggatc
     1621 aatgcgggcg tctgaggctc tccagtcatc gacctatcac gccagtgatg gggtggccta
     1681 tgatcgttgg ctcggccgct gggccgagcg cctggccgag gcggtcatcg atttcgccga
     1741 gtttcccggc gacggcgatc tcctcgacgt tggctgtggc accggcgcgc tgacctttgc
     1801 gctggccaag cgttggccgc agcggcgcgt gatcgggctc gacctggccg agccgtttat
     1861 cgcccatgcc cgcgcccgcg gcgggctttc tcctgcaccg tcctttgcgc tcggcgatgc
     1921 ctgcgccctg ccttacgacg acggccaatt tgccggcgcc gccagccaac tcgtgttgat
     1981 gttcatcccc aaacccgagc gcgcgctgcg ggaaatgctg cgcgtcgtgc ggcccggcgg
     2041 cacgatcgcc gccgccctat gggatttccg cggcgggctc acctttcagc gcctgctctg
     2101 ggacacggcc gcggccctcg atcccgaagc gcgcgccatg cgcaatcggc tgttctcggc
     2161 gcctctggtg ctccccgacg gcttgcgcca cctgttccag gccggcggcc tcgaagacgt
     2221 cgagcggcaa tcgctgaccg ttcgcatgga cttcgccgat ttcgacgact actggcagcc
     2281 gtttctgggg ggacagggtc ccgtcggcgg ctactttgcc aagctcgatc ccgagctcaa
     2341 ggtgcgcatc aaggccgccg tcgccgacgc ctactgctgt ggcgcccccg atggtcccag
     2401 atcgctgacc gctaccgcct gggcggtgcg cggcagggtg ccgtgacgca cctttgccgt
     2461 ctcgtccctt caaccccgcg aggtcgatca tggtcaccgc cgtagcaatg gacgtctcga
     2521 tccccgcgcg gggcgtgcgc tggacgcagc tcgggctcgg catcctttgc atggtcatgg
     2581 tcgccaatct gcaactggga tggacgccct ttgtcgatcc catcgaccag aaataccact
     2641 ggggtcgcta cgccatccaa tgggctttca cgatctttgt gctgacgcaa acctggttgg
     2701 tgccgatgca cggctacctt gccgaccgct tcggcccgcg cgtcatgatc agcagcgccg
     2761 ggctgttggt cggcgccgcc tgggtgctca atgcctacgc cgataccttg ctgctgctct
     2821 acctggcggc cgtgatcggc ggcctcggtg ccggcatcgt ctacggcggc gcggttggca
     2881 atgccctgaa gtggtttccg gatcgccgcg gcctagccgc gggcttgacg gcgtcggggt
     2941 atggcgccgg ctcggcgctg accgtgctgc ccatccagag caccattgca aactggggtt
     3001 acgagaccgc ctttttttgg ttcgggcttg ggcaaggcat cgtcgtgctg ctggtcggac
     3061 tgttgctgcg cgcgcccggc gccagcgaag ttccaaatac cgcggcggcc gtgccgcaag
     3121 cgcaacgcga ctttacgctg cccgaggtgg tgcgatcgcc gcccttctgg gtgatgtatg
     3181 cgatgttcgt aatggtgggg gtggggggac tgatggcgca ggcgcagctc ggccccatgt
     3241 ccaaggattt caagatcgat agcgtcccgg tgaccctcct gttcttgaca ctgccggcgc
     3301 tgacctttgc cctctcgctc gatcgcgtgc tcaacggttt gacgcggccc ttcttcggct
     3361 ggatctccga ccgtatcggc cgggagcaca cgatgttcct ggccttcctg ctcgaggcgc
     3421 tcgccgtcta tgccatgctg accttcgccc acgatccctt gctgttcgtg atcctgtcgg
     3481 gccttgtgtt cttcgcctgg ggcgagatct atgccctgtt ccccgcgctg tgcacggacc
     3541 tctacggccg caaatttgcc acggcaaact acggcctact ctataccgcc aagggcatgg
     3601 cggcgctcgt catccccttg gccaacctgt tgccctcggg acccggcagc tggaagctcg
     3661 tctttatggt tgccgctggg cttgacctcc tcgccgcgct gctggcgttg ttggtcttaa
     3721 agccgctgcg caagcggcac ttggctccga ccggcgccgg cgaggagcga ccgccggcaa
     3781 tgccggcaag cccttgaggc ggcagcctca ccaggttgtc tcgcgcgccg ctgagcccgg
     3841 gccaaccccg ggcaagcacc gccgctttgt cgcggcggcg agagcgcggc gaagaacggc
     3901 ggccgccgcg cgccatcgaa cggccgcttc tggcgccgcc tgggtttagc gaaccaggca
     3961 cggtttcttg gcgtcgaagc tccagccggg gatcaggtat tgcatggcca tcgcatcgtc
     4021 gcgcgcgccg agcgcctgcc ttttatagag ccggtgcgcg tcctcgacgc gttgccgatc
     4081 gagcctgatg ccaagccccg gccgggccgg cacctccagg gcgccgcccc tgatctcgag
     4141 cggctccaag gtcaagcgct gtccatcctg ccagatccag tgcgtgtcga tggccgtaat
     4201 cgaccccggc gccgcggccg ccacgtgcgt gaacatggcg agcgacacgt cgaaatggtt
     4261 gttggagtgc gaaccccagg tcagccccca ggcggcgcag agttgggcga cccgcaccga
     4321 gcccgccagg ccccagaaat gcggatcggc caggggaata tcaacggcgt tgagttgcat
     4381 ggcatggccc agctcgcgcc aatccgtggc aatcatgttg gtggcggtct tcaagccggt
     4441 ggcgcggcgg aattcggcca tggtctcgcg tccggaaaat cccccctccg caccgcaggg
     4501 gtcctcggca taggccaaca cgcccttgag gggctggcag agacggatcg catcctcgag
     4561 ccgccagccg ccgttcggat caagcgtgat gcgggctgcg gggaagcgct cggccaaggc
     4621 gttgaccgtc tcgatctcct cctccccgcg caggacgccg cctttcagct tgaagtcgct
     4681 gaaaccgtat tttgcttgcg ccgcttcggc gagccgcacg atcgcggcag gcgtcagggc
     4741 ttcctcgtcc cgcagccggt cccatgcgtg ggctgtcgcg ggcgcggctc ggtagggcag
     4801 gtcggtcttg cggcgatccc cgataaagaa caaatagccg agcgcgggca cggtttggcg
     4861 ctgctggcct tcgcccagca gggcggcaac cggcacgccg aggcactggc ccaacaaatc
     4921 caacagcgcc gcctcgagcg cggtcaccgc gtgaatggtc gtgcgcagat caaaggtctg
     4981 ctggccacga ccggcccgat cccgcgccgc aaacgccttg tgcacggcgg cgaggatggc
     5041 gttgtagtcg ccaagcgagc gcccgagcac gagaggcttg gcatcctcga tcgtccggcg
     5101 aatggcctca ccgcccggca cttcgccaac gcccgtgcgc cccgcattg
//
LOCUS       DASTMT010000655         1716 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_5554815, whole genome shotgun sequence.
ACCESSION   DASTMT010000655 DASTMT010000000
VERSION     DASTMT010000655.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 1716)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1716)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1716
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5554815"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(8..1546)
                     /locus_tag="VFR00_15155"
     CDS             complement(8..1546)
                     /locus_tag="VFR00_15155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012043075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HEU0160659.1"
                     /translation="MTQLLRGLASGERCLAHADLDLRLRRIASGLLALGVKAGDCIAI
                     LMRNDIAFIEASFAAQTVGAYAVPINWHFKAEEIAYILADCGAKVLFGHADLLAPIAN
                     GLPPGLTTIAVATPAEVAAAYGIAPLAAAAVPGALDYEAWLGVEPAYRGPGGTQPLTM
                     FYTSGTTGVPKGVRRPAPTAAQMAASDRLRRDIFGIVPQIRSAIPGPLYHSAPNAFAM
                     RAGRVGDLMVLLPRFDPVALLQLIEDARIDTMFMVPTMFVRLLKLPAAVKRRYDVSSL
                     KFIIHAAAPCPHDVKQAMINWWGPVIHEFYGSTESSAVTFATSADTLAKPGTVGRAVV
                     GAELRILDDSGKVLPAGEVGEIFTRFAELPDFTYHNKPGQRAVIERDGFITSGDVGYL
                     DADGYLFLCDRKRDMVISGGVNIYPAEIEAVLLRLDGVKDCAVFGIPDAEFGETLMAV
                     VEPADDSGLTPAAIQAHLTRHLAGYKVPRVIELGRELPREDSGKIFKRRLRDPFWHGL
                     GRKI"
BASE COUNT          319 a          535 c          586 g          276 t
ORIGIN      
        1 tcgacgctca gattttgcgg ccgagcccgt gccagaaggg atcgcgcaag cgacgcttga
       61 atatcttgcc cgagtcctcg cggggcagct cgcggcctaa ctcgatgacg cgcggcacct
      121 tgtagccggc gaggtggcgc gtcagatgcg cctgaatggc ggccggcgtc aggccgctgt
      181 cgtcggccgg ttcgaccacc gccatcaagg tctcgccgaa ctcggcatcg ggaatgccga
      241 acacggcgca atccttgacg ccgtcaagac gcagcagcac ggcctcgatc tcggccggat
      301 agatgttgac gccgcccgaa atcaccatgt ctcgtttgcg atcgcacagg aacaggtagc
      361 cgtcggcatc gagatagccg acgtcgcccg aggtgatgaa gccgtcacgc tcgatgacgg
      421 cgcgctgccc gggcttgttg tggtaggtga aatccggcaa ttcggcaaag cgcgtgaaga
      481 tctcgccgac ttcgcccgcc ggcagcacct tgccgctatc gtcgagaatg cgcaattcgg
      541 cgccgacgac ggcgcgtccg acggtgccgg gtttggccag tgtgtcggcg ctggtggcaa
      601 aggtcaccgc actcgattcg gttgaaccat aaaactcatg gataaccggt ccccaccagt
      661 tgatcatcgc ttgcttgacg tcgtgcgggc atggcgccgc cgcgtggatg atgaacttaa
      721 gcgaggacac atcatagcgg cgcttgaccg ccgccggcag cttgagcaaa cggacaaaca
      781 tggtcggcac catgaacatg gtatcgatgc gggcgtcctc gatcagctgc aagagggcga
      841 cgggatcgaa gcgtggcagc agcaccatca ggtcaccgac gcggccggcg cgcatggcga
      901 aggcgttggg ggcagagtga tagagcgggc cgggaatggc cgagcgaatc tgcggcacga
      961 tgccgaaaat gtcgcgtcgc aggcggtcgg aggccgccat ttgcgccgcc gtcggcgccg
     1021 ggcgtcgtac gcccttgggc acgcctgtcg tgccggaggt ataaaacatg gtcagaggct
     1081 gggtgccgcc cgggccgcga taggccggct caacgccgag ccaggcttca tagtcaaggg
     1141 cgcccggcac cgccgcggcg gccaaggggg caatgccgta ggccgccgcc acctccgctg
     1201 gcgtggcgac ggcgatcgtg gtcagcccgg gcggcagccc gttggcgatc ggggccagca
     1261 gatcggcatg gccaaacaag actttggcac cgcagtcggc caagatgtag gcgatctcct
     1321 cggccttgaa gtgccaattg atcggcaccg cgtaggcgcc caccgtctgg gcggcaaacg
     1381 atgcctcgat gaaggcgatg tcattgcgca tcagaatggc gatgcaatca cccgccttga
     1441 cgccaagcgc gagcaagccg gaggcaatgc gtctgaggcg cagatccaag tcggcgtgtg
     1501 cgaggcagcg ctcgccgctg gcgagcccgc gcaacagctg ggtcatgatc gctcgatcgc
     1561 tacgttctgg tggcgaggag cttagcatcg cgtgccgccg acgccagcgg cacaaaggat
     1621 ggcgaacccc tcacccgtag ccgccgttgt ccgcctctag tagggtatga gacagatagg
     1681 cgggccctgg agcttgcctt gccggtcctg gcttac
//
LOCUS       DASTMT010000656         3387 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_5234248, whole genome shotgun sequence.
ACCESSION   DASTMT010000656 DASTMT010000000
VERSION     DASTMT010000656.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3387)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3387)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3387
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5234248"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..703)
                     /locus_tag="VFR00_15160"
     CDS             complement(<1..703)
                     /locus_tag="VFR00_15160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008523093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-hydroxylase N-terminal
                     domain-containing protein"
                     /protein_id="HEU0160660.1"
                     /translation="MPEVPERLLAGKQRPFTGAEYLESLRDGREVYIYGEKVRDVTRH
                     PAFRNAAVSVAKLYDALHAAKSKDVLTTETDTGSGGYTHRFFKAARSRQDLIGQRDAI
                     ATWARITYGWMGRSPDYKAAFLNTLGANAEFYGKFAANARAWHVRGQEAVLYVNHALV
                     NPPIDRSKPADQVKDVYISIQKETDAGIVVSGAKVVATNSALTHYNFLGQNMGQDIND
                     DSMVVMFMAPMDTPGI"
     gene            774..1688
                     /locus_tag="VFR00_15165"
     CDS             774..1688
                     /locus_tag="VFR00_15165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015886715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0018576 - catechol 1,2-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0009712 - catechol-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dioxygenase"
                     /protein_id="HEU0160661.1"
                     /translation="MIIDNEASVTTAVLEAFGGTEPPRRREILMALVRHLHGFVREVR
                     LTEAEFHEAIRLLAAVGQHTTAWHNEAMLMAGSLGVSALVCLINNGNAGRTETQANLL
                     GPFWRDDQPICNSGDSIVRSPTAGPPLICRVEVQDAAGQPVAAAEVDVWHSSPEGLYE
                     NQDPRQAAMNLRGRFLTDAAGRFEFRSLKPAGYPVPIDGPVGELIAATGRHNFRPAHL
                     HFMVYKPGFKTLISQIYTPDDPHLETDVQFGVTRALIGNFVRHDTAEPGLGFAAPWYS
                     LDQRLRLEPGQAQRPRAPIRAKAPAAAG"
     gene            complement(1720..2859)
                     /locus_tag="VFR00_15170"
     CDS             complement(1720..2859)
                     /locus_tag="VFR00_15170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017486115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aspartate aminotransferase family
                     protein"
                     /protein_id="HEU0160662.1"
                     /translation="MKRNALAPETVAAQAGEAGDETTGATVPPVHLATTFLRDPDNQY
                     RKGFSYGRPDNASVRQVERVLGELEGGAASLVVASGMTAATTAFLALERPAHVVAPNV
                     MYWGLRDWIARDAPSLGIEPTFVDATDPAAIAAAVRPGHTKLVWIETPANPLWSLSDI
                     AAAAKIAHTAGALLAVDSTVSTPVLTQPLTYGADIVMHSATKYLNGHSDVMAGALVFA
                     RRDAYFERASRSRTLLGGILGPFEAALLLRGMRTLHVRVRHQSAAAMAIASHFQGDRR
                     LEAVLYPGSPAHPGHAIARRQMQGGFGGMLSLRVRGGETAAVAVAAKVRVWKRATSLG
                     GVESLIEHRASIEGPASPCPPDLLRLSVGLESTLDLIDDLEQALS"
     gene            complement(2921..>3387)
                     /gene="rlmH"
                     /locus_tag="VFR00_15175"
     CDS             complement(2921..>3387)
                     /gene="rlmH"
                     /locus_tag="VFR00_15175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011313778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEU0160663.1"
                     /translation="AVGRLKDGPERLLFERYRARFEATGRRLGFAPVLVGETPESGAG
                     TPGQRQREEAGALLKLTREAETLIALHGAGKACSSEGLAQLLRNVRDAGSKTMAVVVG
                     GPDGLGADLLARAGHKISFGPITLPHQLARIVLAEQLYRAATILAGHPYHRP"
BASE COUNT          561 a         1132 c         1124 g          570 t
ORIGIN      
        1 tgatgccggg cgtatccatt ggcgccatga acatcacgac catgctgtcg tcattgatgt
       61 cctggcccat gttttgaccg agaaagttgt agtgtgtcag cgccgagttc gtggcgacga
      121 ccttggcgcc cgacacgacg atccccgcat cggtttcctt ctggatcgag atatagacat
      181 ccttcacttg gtcggcgggc ttggaccggt cgatgggcgg attgaccaag gcgtgattga
      241 cgtagagcac ggcctcctgg ccgcgcacgt gccaggcccg cgcgttagcc gcaaacttgc
      301 cgtagaactc cgcattggcg cccagcgtat tgaggaaggc agccttgtag tcgggactgc
      361 ggcccatcca gccataggtg atgcgcgccc aggtggcgat ggcatcgcgt tgcccgatca
      421 aatcttgccg cgaacgcgcc gccttgaaga agcgatgggt gtagccgccc gagccggtgt
      481 ccgtctcggt cgtcagcacg tctttggatt tggcagcgtg cagggcgtcg taaagtttgg
      541 ccacggaaac cgccgcattg cggaaggctg gatgccgcgt gacatcgcgc accttctcgc
      601 cgtagatata aacctcgcgg ccgtcgcgca gggattccaa atactcagcg ccggtaaagg
      661 gccgctgctt gcccgcaagc agacgctccg gaacctcagg cattgctttg acccacctgg
      721 tgaagacgcg ctcggatgtg ggttagcata acggcagcag gagaggtcga gaaatgatca
      781 tcgacaacga ggcctccgtc acgacggccg tactcgaggc cttcggtggc accgagccgc
      841 cgcgccgccg cgagattctc atggctttgg tgcgtcatct gcacggcttc gtgcgcgagg
      901 tgcgcctgac ggaggccgaa ttccacgaag cgatccggct cctcgccgcc gtcgggcaac
      961 atacgaccgc ctggcacaac gaggccatgc tgatggcagg gtcgctgggc gtgtcggcgc
     1021 tggtttgcct catcaacaac ggcaacgcgg gccgaaccga gacccaggcc aatctgcttg
     1081 gaccgttctg gcgtgacgac cagcccatct gcaacagcgg cgacagcatc gtgcgctcgc
     1141 caacggcggg gcctccgctc atctgtcgcg ttgaggttca ggacgccgcc gggcagcccg
     1201 ttgccgctgc cgaggtcgac gtctggcact cctcgccgga gggcctctat gagaaccagg
     1261 accctcgcca ggccgccatg aacctgcgcg gccgcttcct caccgatgcg gcgggccggt
     1321 ttgagttccg cagcctgaaa cctgccggct acccggtacc catcgacggc cccgtcggcg
     1381 agctgattgc ggccacgggc cggcacaatt tccgccccgc ccacctgcac ttcatggtgt
     1441 acaagcccgg cttcaagacg ctgatttcgc agatctatac gccggatgac cctcacctcg
     1501 agacggatgt gcagttcggc gtcacgcgcg ccctgatcgg caatttcgtg cggcacgaca
     1561 cggccgagcc ggggctcggt tttgcggcgc cctggtattc cctggatcag cgcttgcggc
     1621 tcgagccggg gcaagcccaa cgtccccgcg cgccgatccg cgccaaagcg cccgcggccg
     1681 ccggctaacc cccggccacc gccgcggcag accttgagct caagacagcg cttgttcgag
     1741 atcgtcgatc aaatcgagcg tgctctcgag gccgaccgag agccgcaaca ggtcgggcgg
     1801 acagggcgac gcggggccct cgatgctggc gcgatgctcg atcaggcttt cgaccccgcc
     1861 gagcgaggtt gcccgcttcc acacgcgcac cttggccgcc acggccaccg ccgccgtctc
     1921 gccaccccgc acgcgcagcg agagcatgcc gccgaagccg ccctgcatct ggcgtcgcgc
     1981 gatggcatgg cccgggtgcg ccggcgaacc gggatagagg accgcctcca ggcggcgatc
     2041 gccctggaag tggctggcga ttgccatggc cgcagccgac tgatgacgca cgcggacgtg
     2101 caaggtgcgc ataccgcgca aaagcaatgc cgcctcgaag ggcccgagaa tgccccccag
     2161 aagcgtgcgc gagcggcttg cccgctcgaa ataagcgtcg cgacgggcaa acaccaaggc
     2221 cccggccatg acgtcggaat ggccgttgag gtacttggtc gccgaatgca tcacgatgtc
     2281 ggcgccatag gtcagcggtt gggtcaggac aggggtcgac accgtggagt ctacggccag
     2341 cagcgccccg gccgtgtgcg caatctttgc cgccgcggcg atatcgctga ggctccaaag
     2401 agggttggcg ggggtttcga tccacacgag cttggtatga ccgggtcgga ccgcggccgc
     2461 gattgcggcc gggtcggtgg catcgacgaa ggtgggctcg atgcccaggc tcggggcgtc
     2521 ccgggcgatc caatcgcgca ggccccaata catgacgttg ggggccacga cgtgtgccgg
     2581 tcgctccagc gccagaaaag ccgtggtcgc cgccgtcatg cccgaggcga ccaccagcga
     2641 cgccgcgccg ccttccagtt cccccagcac ccgctcgact tgccgcacgc tggcgttgtc
     2701 cgggcggccg tagctgaagc ctttgcggta ttgattgtcg gggtcgcgca ggaaggtggt
     2761 ggccaggtgc acgggcggga ccgtcgcacc ggtcgtctcg tccccggcct cgccagcctg
     2821 ggcggcgacg gtctccggcg ccagggcatt cctcttcatg atcggctcgc agttcggcgg
     2881 ttgcaggttg gcatggagcc ggcccggggt tggttcactg tcatgggcga tggtaggggt
     2941 ggcccgcaag gatggttgcg gcacggtaga gctgttcggc aagcacaatt cgcgcaagtt
     3001 gatgcggcaa tgttatcggg ccaaaggaaa tcttgtgccc ggcccgggcc aaaaggtcgg
     3061 cgcctagtcc gtcgggaccc ccgaccacca cggccatggt cttactgccg gcatcgcgca
     3121 cgttgcgcaa aagctgcgcc aagccctcgc tgctgcaggc tttgccggct ccgtggaggg
     3181 cgatcagcgt ctcggcctcg cgcgtcagct tgagaagcgc gccggcttcc tctctttggc
     3241 gctgtccggg ggtgccggcg ccgctttccg gcgtctcgcc caccaggacg ggcgcaaaac
     3301 cgagacgtcg cccggtcgcc tcgaaccgag cacgatatcg ttcgaacaac agccgttccg
     3361 gtccatcctt gagccggccg acggcgg
//
LOCUS       DASTMT010000657         5750 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_3908431, whole genome shotgun sequence.
ACCESSION   DASTMT010000657 DASTMT010000000
VERSION     DASTMT010000657.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 5750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5750
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3908431"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..444
                     /locus_tag="VFR00_15180"
     CDS             <1..444
                     /locus_tag="VFR00_15180"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU0160664.1"
                     /translation="ILPMPPQLESELGVEPVSLEELLARSEILTLHVPVTPQTKKIIN
                     PNTLARLRPGAYLINTCRGGVLDEAALVAALAQGHLAGAALDVFDPEPPPADHALYRF
                     RNVVLTPHISAGTRDAFIAKMTFVFGNLGRFWRGEPVENLVDFGA"
     gene            567..1541
                     /locus_tag="VFR00_15185"
     CDS             567..1541
                     /locus_tag="VFR00_15185"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018238807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine dehydratase"
                     /protein_id="HEU0160665.1"
                     /translation="MPLFTLAELEAALPLVRSVVPATPQHAWPLLAARAGAEVWVKHE
                     NHTPIGAFKVRGGVTYLDALQRGGQRFAGVVSATRGNHGQSIALAAARANLPCTIFVP
                     HGNSREKNAAMQALGAKLVVIGADFDACRPHAATFAAEHGYHYVPSFHADLVKGVATY
                     ALELFGAVADLDAVYAPIGMGSGICGLISVRDLLGLTTEIIGVVADSAPAVALSVEQG
                     RLVNTNCAETFADGMACRVPVAEPIEIIRRGAARIVRVTEDEIAAAMRAYYTDTHNLA
                     EGAGAAPLAALLKERARMQGKRVGLILSGGNIDREQFLRVLAGETPSP"
     gene            complement(1559..2851)
                     /locus_tag="VFR00_15190"
     CDS             complement(1559..2851)
                     /locus_tag="VFR00_15190"
                     /EC_number="2.5.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013417859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase"
                     /protein_id="HEU0160666.1"
                     /translation="MPDTPDTAPGFATLSVHAGAAPDPSTGARVTPIYQTTSYVFNDV
                     DHAAALFGLEVFGNIYTRIGGPTQSVLEQRLAALEGGSAALAVASGHAAQAVVFHTLL
                     EPGDEFVAGRQLYGGSVNQFNHAFKKFDWHVQWADATKPESFAKAVTPKTKAIFCESI
                     ANPGGIVVDLPAIAAIAKKANVPFIVDNTLATPYLCRPKEFGADIVLHSLTKFLGGHG
                     NSIGGAIVDCGSFDWLGAQKKYATLVDPNPSYNGMRLAETFGNFAFAIACRVMGLRDL
                     GPAISPFNAFLILTGIETLPLRMAKHCENAVAVANYLERHKGIEWVNYAGLASNPYHG
                     LASKICPKGAGSVFTFGVKGGYDAGVKLVSNVRLFSHLANIGDVRSLIIHPASTTHRQ
                     LTDEQRKAAGAGADVVRVSIGIEDAADIIADLDQALAA"
     gene            complement(2972..3337)
                     /locus_tag="VFR00_15195"
     CDS             complement(2972..3337)
                     /locus_tag="VFR00_15195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0160667.1"
                     /translation="MARTAKRTKAKAGRGLAEATLAAAPEVRSVFIDAAGKRWRWVVD
                     NRMRDYGAINYGRRVIRINRDLHKRERESLIDTLFHEELHRLFPYLGERTICTMTRLL
                     LPTLTRRYRAWLYARIRSR"
     gene            complement(3530..4360)
                     /locus_tag="VFR00_15200"
     CDS             complement(3530..4360)
                     /locus_tag="VFR00_15200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015933387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEU0160668.1"
                     /translation="MLTRAIPQSQEALPVVGFGTWQTFDVGADRSQREERKQVLAALF
                     AAGGKVIDSSPMYGSAEAVVGELLSDMQAHGQAFLATKVWTSGEAAGQRQMQASFSKL
                     KTRRIDLMQIHNLVDWRTQLKTLRAWKAAGRFRYIGITHYTVPELDHLASLIKSERLD
                     FVQFGYSLNVRAAEARLLPLAADRGVAVIINQPFDSGALFGKVRGKALPEWAAEFDCA
                     SWGQFFLKYILGHAAVTCVIPGTAKLEHARDNLGAGVGRLPDASQRQRMSAYWDAGPV
                     "
     gene            complement(4504..4932)
                     /locus_tag="VFR00_15205"
     CDS             complement(4504..4932)
                     /locus_tag="VFR00_15205"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEU0160669.1"
                     /translation="MSAKLPQSIAEWNEAGAAYLPGHLGMRFEKVEPQEVVASFEVRR
                     ALEAWNGYLHAGSVVALADTCCGYGTLRSLPAGATGFTTIELKSNFFGSTRQGVVVCI
                     ARALHQGRTTQVWDASVCRAGEEAPIARFRNTQLILWPKP"
     gene            5065..>5750
                     /locus_tag="VFR00_15210"
     CDS             5065..>5750
                     /locus_tag="VFR00_15210"
                     /inference="COORDINATES: protein motif:HMM:NF009825.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate dehydrogenase"
                     /protein_id="HEU0160670.1"
                     /translation="MPSQRAPLKLAIAGLGAIGLSVARRVDAGDVDGIVLSAVAARDH
                     AKARRAMAEFRSPPPLTTLAGLAEAADIVLECLPAREFLAVAEPAIARGRVLVPLSVG
                     ALLDHWHLVDAAKAKGARILVPTGALLGLDAVRAAAEGNISEVRMVTRKPPTGLAGAP
                     LLKARNISLEGLTAPLQVFQGSAREAIAGFPANVNVAVALSLAGIGPDRTRVEIWADP
                     TLTHNHHTIVV"
BASE COUNT          940 a         1917 c         1946 g          947 t
ORIGIN      
        1 atcctcccca tgccgccgca gctcgagagc gagctcggcg tcgagccggt gagcctcgag
       61 gagctgttgg cgcgctcgga gatcttgacg ctgcacgtgc cggtgacgcc gcagacgaag
      121 aagatcatca acccaaatac cctcgcgcgc ctgcgccccg gcgcctatct catcaacacc
      181 tgccgcggcg gcgtgctcga cgaagcagcg ctggtggccg ccttggcgca aggccatctc
      241 gccggcgcgg cgctcgacgt gttcgacccg gagcccccgc ccgccgacca tgccctctac
      301 cgcttccgca acgtcgtgct gaccccgcat atttccgcgg gcacgcgcga tgccttcatt
      361 gccaagatga cgttcgtgtt cggcaacctt ggccggttct ggcgcgggga gcccgtcgag
      421 aacctggtcg acttcggcgc ctgaggcgat ggcagggtca aggacaaccg gctgtcgccc
      481 aggaacccag gggccgagag gcagaaacgg gcgggaggcc agacggccgg acggccgctt
      541 gctagggtga ggagatgacc aaccccatgc cgcttttcac gttggccgag ctcgaagcgg
      601 ccctgccgtt ggtgcgcagc gtggtgccgg cgaccccaca acacgcctgg ccgctgctgg
      661 cggcgagggc cggcgccgag gtctgggtca agcacgagaa ccacacgccc atcggcgcct
      721 tcaaggtgag gggcggcgtg acttatttgg acgcactgca gcgcggcggg cagcgcttcg
      781 cgggcgtggt gagcgccacg cgcggcaatc acggccaatc cattgccttg gccgccgccc
      841 gcgccaacct gccctgcacg attttcgtac cccacggcaa ctcccgggaa aagaacgccg
      901 ccatgcaggc gctgggcgcc aagctggtgg tgatcggcgc cgatttcgac gcctgccgcc
      961 cgcacgcggc caccttcgcg gccgagcacg gctatcacta cgtgccctcg tttcacgccg
     1021 atctcgtcaa gggggtcgcc acctatgcgc tcgagctgtt tggcgccgtg gccgatctcg
     1081 acgccgtcta tgcgccgatc ggcatggggt cgggcatttg cggcctgatt tcggtgcgcg
     1141 acctgttggg cctaacgacg gagatcatcg gcgtcgtggc ggacagcgcc cccgccgtgg
     1201 ccctctccgt cgaacagggg cgcctcgtca acaccaattg cgccgaaacc ttcgccgacg
     1261 gcatggcctg ccgcgtgccg gtggccgagc caatcgagat catccggcgc ggtgcggcgc
     1321 ggatcgtgcg cgtcacggag gatgaaatcg ccgcggccat gcgggcctac tataccgata
     1381 cccacaacct cgccgagggc gccggcgcgg cgccgctggc ggcgctcctc aaggagcgcg
     1441 cgcgcatgca gggcaaacgc gttggcctga tcctcagcgg cggcaacatc gaccgggaac
     1501 agttcttgcg cgtcctcgcc ggcgagacac catccccctg agcggcgacg ggcaccgctc
     1561 aggcggcaag cgcctggtcc agatcggcaa tgatgtcggc agcatcctcg atgccgatcg
     1621 agacgcgcac cacgtcggca ccggccccgg ccgccttgcg ctgctcatcg gtgagttggc
     1681 ggtgggtggt ggaggcggga tggatgatga ggctgcgcac gtcgccgata ttggccagat
     1741 gcgagaacag cctgacgttg gagacgagct tgacgccggc atcgtagcca cccttgacac
     1801 cgaaggtgaa gacgctgccc gcgcccttgg ggcaaatctt gctggccagg ccgtggtagg
     1861 ggttgctggc aagaccggcg tagttcaccc attcgatgcc tttgtgccgt tcgagatagt
     1921 tggcgacggc aacggcgttc tcgcaatgct tcgccatccg cagcggcagc gtttcgatgc
     1981 ccgtcaggat caggaaggcg ttgaaaggcg aaatcgccgg gcctaagtcg cgcagcccca
     2041 tgacgcggca ggcgatggcg aaggcgaagt tgccgaaggt ctcggccaag cgcatgccgt
     2101 tatagctcgg attggggtcg accagggtgg cgtatttctt ctgcgcgccc agccaatcga
     2161 agctaccgca atcgacgatc gccccgccga tggaattgcc gtgtccgccc agaaacttgg
     2221 tgagcgagtg caggacgatg tcggcgccga actccttcgg ccgacatagg tagggggtcg
     2281 ccagcgtgtt gtcgacgatg aagggcacgt tggccttctt cgcgatcgcg gcaatggccg
     2341 gcaggtcgac gacgatgccg ccggggttgg caatcgattc acagaagatc gccttggtct
     2401 tgggggtcac cgccttggcg aagctctcgg gcttcgtggc atcggcccat tgcacgtgcc
     2461 agtcgaactt cttgaaggcg tggttgaatt ggttgaccga gccgccgtag agctggcggc
     2521 cggcgacgaa ttcatcgccg ggctccagca aggtgtggaa caccaccgcc tgcgccgcgt
     2581 gcccggaggc gaccgccagc gccgcgctgc cgccctcgag cgcggcgagg cgctgctcga
     2641 gcacgctctg ggtgggcccg ccgatgcgcg tataaatatt gccgaacacc tccaggccga
     2701 agagcgccgc ggcgtggtcg acatcgttga aaacatacga agtcgtctga tagatcggtg
     2761 tcacgcgcgc gccggtggag ggatcgggtg ccgcgccggc gtgcacggaa agcgtcgcaa
     2821 atcccggggc ggtgtcgggc gtgtcgggca tcggcggctc ctaaacctca aactcgctgc
     2881 ttggctcaag cgcaacgacc ccaaacgcgc cgacaagtca acgaggaatt tgcagctcct
     2941 tcgcccggct tgacgcgcaa gagccgctgg ctcaacgcga gcggatgcgc gcgtagagcc
     3001 aggcccgata gcgccgggtg agcgtcggca gcaacagccg cgtcatggtg cagatcgtgc
     3061 gctcgcccag gtagggaaag agccggtgca gctcctcgtg aaacaaggta tcgatcaagg
     3121 actcgcgttc gcgtttgtgc agatcgcggt tgatgcggat gacgcgccgc ccatagttga
     3181 tggcgccgta gtcgcgcatg cgattgtcca ccacccagcg ccagcgctta ccggccgcgt
     3241 cgataaagac cgagcgcact tcgggtgcgg cggcgagtgt ggcctcggcg agcccacggc
     3301 cggctttggc cttcgttctt tttgctgttc ttgccatcgc tcaacctcct cgcgcatctt
     3361 actcgaggag gcaaggtgct gaccggttgg gcacccatgt ggcaatggtt tcctcagcaa
     3421 cgcgccggcc tcgggccgaa aaactaagcc cctctttcta cacgaaaaaa ctcggttttc
     3481 tcgcaaaaat tgctgagcct gagttcggcg ccgtacggta gtaaaggcct caaaccgggc
     3541 cggcgtccca ataggcgctc atgcgctggc gctggcttgc gtccggcagc cgcccgaccc
     3601 ccgcccccag gttgtcgcgg gcgtgctcga gcttcgccgt accggggatg acgcaggtca
     3661 ccgccgcatg gcccaggatg tatttcagaa agaactgtcc ccagctggcg caatcgaact
     3721 ccgcggccca ctccggcagc gccttgccgc gcactttgcc gaacagggca ccgctgtcga
     3781 agggctggtt gatgatgacg gcgacgccgc ggtcggcggc gagcggtaat aggcgcgcct
     3841 cggcggcgcg cacgttgagc gaatagccaa attgtacgaa gtcgagccgc tcgcttttga
     3901 tcaggctggc gaggtggtcg agctccggca ccgtatagtg ggtgatgccg atatagcgaa
     3961 agcgtcccgc cgccttccag gcccgcagcg tcttcagctg cgtgcgccag tcgaccaggt
     4021 tatggatctg catcagatcg atgcggcgcg ttttgagttt gctgaaggag gcctgcatct
     4081 ggcgctgtcc ggcggcttca ccgctggtcc agaccttggt ggcgaggaag gcctgcccat
     4141 gcgcctgcat gtcgctcagg agctcgccaa ccacggcctc ggcgctgccg tacatcggcg
     4201 aggaatcgat caccttgccg ccggctgcga acagcgcggc cagcacttgt ttgcgctctt
     4261 cgcgttgact gcggtcggca ccgacatcga aggtctgcca ggtgccgaag cccacgaccg
     4321 gcaaggcttc ctggctttgt gggatggctc gcgtcagcat ggcttggctc gttgcttgcg
     4381 gcgcgctcgc cgcgccttgc gggcgggccc aaacggcccc acagcaggcc gccgcgccga
     4441 ggccgatcag gcggcggcgg ctgggcatgc ggctgggcat gaagcgagct gccggacgcg
     4501 ggctcatggc ttgggccaca ggatcagttg cgtgttgcgg aagcgggcga tcggcgcctc
     4561 ttcgccggcg cggcagacgc tggcgtccca cacctgcgtg gtgcggcctt ggtgcagcgc
     4621 ccgggcgatg cagaccacca cgccctggcg ggtcgagccg aagaagttgc tcttcagttc
     4681 gatggtggta aagccggtcg cacccgcggg caggctgcgc aaggtaccat agccacaaca
     4741 ggtgtcggcc agcgccacca cgctgccggc gtgcagatag ccgttccagg cctcgagcgc
     4801 gcggcgcacc tcaaagctgg cgaccacctc ctgcggctcg actttctcaa accgcatgcc
     4861 gaggtgcccc ggcagatagg cggcaccggc ctcattccac tcggcgatcg attggggcag
     4921 cttggcggac aaaagagctc tcctgacagg gggacaacgg cgggctagtc tcgcagaagg
     4981 gtggccgcag gacacctcaa ttccgcacgg ggttcgatgc aagggccacg ccggcgcgaa
     5041 acgcccgtcc cactggaggc aaccatgcca agccaacgcg cgcctctcaa gcttgccatc
     5101 gccggcctcg gcgccatcgg gctcagcgtg gcacgccgcg tcgatgccgg cgacgtcgac
     5161 ggcatcgtct tgagtgcggt ggcggcccgc gaccacgcca aggcgcgccg ggcgatggcc
     5221 gaattccgct cgccgccgcc gctgacgacg ctcgccgggc tggccgaggc ggccgatatc
     5281 gtccttgaat gtctgcccgc gcgcgagttt ttggcggtgg ccgagccggc catcgcccgc
     5341 ggccgtgtgc tggtcccgct ctccgtcggg gcgctgctcg atcactggca tctggttgac
     5401 gccgccaagg ccaagggcgc ccgcatcctg gtgccgaccg gggcactctt gggtctcgac
     5461 gcggtgcgcg cggccgcgga gggcaacatc agcgaggtcc gcatggtcac gcgcaagccc
     5521 ccgacgggcc tggcgggcgc tccgctgctc aaggcacgca acatctcgct cgagggacta
     5581 acggcgccgc tgcaggtctt ccagggcagc gcccgcgagg cgattgccgg ctttcccgcc
     5641 aacgtcaacg tcgccgtcgc cctgtcgttg gcgggcatcg gtcccgatcg cacgcgtgtc
     5701 gaaatctggg ccgacccgac gctgacgcac aaccaccata cgatcgtggt
//
LOCUS       DASTMT010000658         3082 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_5235277, whole genome shotgun sequence.
ACCESSION   DASTMT010000658 DASTMT010000000
VERSION     DASTMT010000658.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3082)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3082)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3082
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5235277"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..429)
                     /locus_tag="VFR00_15215"
     CDS             complement(<1..429)
                     /locus_tag="VFR00_15215"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEU0160671.1"
                     /translation="MLAAWQPDSWRSKPIVQVPDYPDVAALAEVERNLRNFPPLVFVK
                     EVRDLKEKLAGVADGKAFLLQGGDCAESFKEHKTDYIRDYFQLFLQMALILTFAGSSQ
                     VVKIGRVAGQFAKPRSSPTEKKDGRELPSYRGDIINGPEFT"
     gene            616..1515
                     /locus_tag="VFR00_15220"
     CDS             616..1515
                     /locus_tag="VFR00_15220"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEU0160672.1"
                     /translation="MMPVDDDGAATVRPFTLAADDGYALAATEFCGPTPAAGSPLTVI
                     APAAAVPARYYARFASYLAVRGGGPVVTFDYRGIGGSAPKTLRGFPARMRDWCCLDAA
                     GAIGWARQTHPERPLHWLGHSMGGFAPGLAHNNGAIARQLNVATLSGYWGRMRSPERY
                     RVRFLMGSAAPPVVRLLGFFPGVLMGGENMPAPAFLEWTRWCMLPDFIFGDPTLREVR
                     HFATLKAPVRFAQIEDDVWGTPAAVGHMASHFSASSERSIWRVSLAEAKAKRIGHLGF
                     FRSAFRATLWPAAAAWLEGSAGD"
     gene            1649..2779
                     /locus_tag="VFR00_15225"
     CDS             1649..2779
                     /locus_tag="VFR00_15225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009757951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate-amine ligase"
                     /protein_id="HEU0160673.1"
                     /translation="MAFKDPSFSIGIEEEYLLVDRATRDLVRDMPQALFDRAQQALQG
                     QVAREFLKSQIEVGTSVHKSPGQAGAELAHLRRTVAELAAEHGLAPIAASTHPFARWS
                     TQEPTERARYQAIAKDLAGVGRRLVICGMHVHVGIEDDELRIDLFNQARYFLPHLLVL
                     STSSPFAEGEDTGLKSYRLAAYQELPRTGMPGRFEGWSEYLQTVDLLVRNGIIEDASK
                     IWWDIRPSSRFPTLEMRVTDVCPLVEDAVCVAALYLCILRMLYRLRRANQTWRTYPLF
                     LLAENRWRAQRYGVEGSLFDLGKGELVPFRDLVDELLVMLREDAEALGCLAELEHART
                     ILARGTSADRQVAHFQAQLGAGATREDALASVVDHLIKETVA"
     gene            complement(2812..3018)
                     /locus_tag="VFR00_15230"
     CDS             complement(2812..3018)
                     /locus_tag="VFR00_15230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0160674.1"
                     /translation="MPAETPAPKAEQPANAEEPAKWRALAAEARACAAAMAEPRARRL
                     MQDIAAAYEKLAQRADSQSPRASN"
BASE COUNT          461 a         1083 c          989 g          549 t
ORIGIN      
        1 cgtgaactcg ggaccgttga tgatgtcgcc gcggtagctt ggcagctcgc ggccgtcctt
       61 tttctccgtc ggcgaggagc gcggcttggc gaactggccc gccacgcgcc cgatcttgac
      121 gacctggctg ctgcccgcga aggtcaaaat cagcgccatt tgcaggaaca gctgaaaata
      181 atcgcggatg tagtcggtct tgtgctcctt gaagctctcc gcacagtcgc cgccctgcag
      241 caggaaggcc ttgccgtcgg cgacaccggc aagcttctcc ttgaggtcgc ggacctcctt
      301 gacgaagacc agcggcggga agttgcgcaa attgcgctcg acctcggcca aagccgccac
      361 gtcagggtag tcggggacct ggacgatcgg cttcgatctc cagctatcgg gctgccacgc
      421 cgccaacatt tgcgtcttct ccggacaaaa tcgcatcggc cccccggggc aatgtccctg
      481 ggccagcaag agaatagtgg ggtctcgggc ggcgttatag tggaaagccg caagcgccac
      541 cagggcctgt ggcttgctct tgcagccggg cgcgggtcca tgctgcgcga acataacaag
      601 gattagcagg accgcatgat gcccgtggac gacgacggcg ccgcgacggt gcgccccttc
      661 acgctggcgg ccgatgacgg ctacgcattg gccgcaaccg aattctgcgg cccgacgccg
      721 gccgcaggca gccccctgac agtgattgcc cccgccgccg ccgtacccgc ccgctactac
      781 gccaggtttg ccagctatct ggccgtgcgc ggcggcggac cggtcgtcac cttcgactac
      841 cgtggcatcg gcggctctgc ccccaagacc ttgcgcggct ttcccgcccg catgcgcgat
      901 tggtgctgcc tcgatgccgc cggcgccatc ggctgggcgc ggcaaaccca ccccgagcgt
      961 ccactgcact ggctgggcca tagcatgggc ggcttcgcac ccggtctcgc ccacaacaac
     1021 ggcgccattg cccgccagct caacgtcgcc accctcagcg gctactgggg ccgcatgcgc
     1081 agcccggaac gctaccgcgt gcgctttttg atgggctcgg cggcgccgcc cgtcgtgcgc
     1141 ttgctcggct ttttcccggg ggtgctgatg ggcggggaaa atatgccggc gcccgccttt
     1201 ctcgaatgga cccgctggtg catgctgccg gactttattt ttggcgaccc gacgttgcgc
     1261 gaagtccgcc acttcgccac gctcaaggcc ccggtccggt ttgcccagat cgaggacgac
     1321 gtctggggca cgccggccgc cgtcggccac atggcgagcc atttttccgc cagcagcgag
     1381 cgcagcatat ggcgggtgag cctggccgag gccaaggcga agcgcatcgg ccatctcggc
     1441 ttcttccgct ccgccttccg cgccaccctg tggcccgccg ccgccgcttg gctcgagggc
     1501 agcgccgggg attagcgcaa aagggctttc gccgctggcc cgtgccaccc ccttgggcgg
     1561 gccgacgggg ccgcctcatc cccgacattc ccaggctccg ttggcggccg tcgtttgatg
     1621 ggcggccaat tcgtgccaac atttccccat ggccttcaag gatccttcct tcagtatcgg
     1681 catcgaggag gaatacctcc tggtcgatcg cgcgacgcgc gacctcgtgc gcgacatgcc
     1741 gcaggcgctg ttcgatcggg cccagcaggc cctgcagggc caggtggcgc gcgagttcct
     1801 caagtcccaa atcgaggtcg gcaccagcgt gcacaaatcg ccgggccagg ctggcgccga
     1861 actcgcccat ttgcgccgca cggtggccga gcttgccgcc gagcacggcc tggcgccgat
     1921 tgcggcctcg acccatccct ttgcgcgctg gagcacgcaa gaacccaccg agcgggcgcg
     1981 ttatcaggcg attgccaagg atctcgccgg cgtcggccgc cgcctagtga tctgcggcat
     2041 gcacgtacac gtcggcatcg aagacgacga gttgcgcatc gacctcttca atcaggcccg
     2101 ctatttcctg ccgcacctgt tggtgttgtc gacgtcctcg cccttcgcgg aaggcgaaga
     2161 cactggtctc aagtcctacc ggctggcggc ctaccaggag ctgccgcgca cgggcatgcc
     2221 ggggcgattc gagggctggt ccgaatacct acagaccgtc gatctgctcg tacgcaacgg
     2281 catcatcgag gacgccagca agatttggtg ggacatccgt ccctcgtcgc gctttcccac
     2341 cttggaaatg cgcgtcacgg acgtctgtcc cctggtcgag gatgccgtgt gcgtcgccgc
     2401 cctctatctg tgcatcctgc gcatgttgta tcggctgcgg cgcgccaacc agacctggcg
     2461 cacgtatccc ttgtttcttc tggccgaaaa ccgctggcgc gcgcagcgct acggcgtgga
     2521 gggaagtctg ttcgacctcg gcaaggggga actcgtgccg ttccgcgacc tcgtcgatga
     2581 actgctggtg atgttgcgcg aggacgccga agcgctgggc tgtctcgccg agctcgagca
     2641 cgcccgcacc attctggcgc gcggcaccag tgcagaccgg caagtcgccc atttccaggc
     2701 gcaacttggc gccggcgcca cgcgcgagga tgcgcttgca agcgtcgtcg atcacctgat
     2761 caaggaaacg gtggcctgag caggcaccgc ggcgctgggc ggcgctccac ttcaattgga
     2821 ggcccgcgga ctttggctgt cggcgcgctg cgccagcttc tcgtaggccg cggcgatgtc
     2881 ctgcatcagc cgtcgcgccc gcggctcggc catcgcggcc gcgcaggcac gcgcctcggc
     2941 ggcgagcgcg cgccactttg ctggctcctc ggcgtttgct ggctgctcgg cttttggcgc
     3001 tggcgtctcc gccggcatgg ccgctgtccc tctcccacgc cagcttgatt aacgcggcga
     3061 acgcgcaacg agttcctgcg gc
//
LOCUS       DASTMT010000659         4590 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_5562353, whole genome shotgun sequence.
ACCESSION   DASTMT010000659 DASTMT010000000
VERSION     DASTMT010000659.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4590
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5562353"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            44..1021
                     /locus_tag="VFR00_15235"
     CDS             44..1021
                     /locus_tag="VFR00_15235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012109984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HEU0160675.1"
                     /translation="MSLNRSPLPQEGGGLFLTDGGIETTLIFHEGWELPHFAAFDLLQ
                     TPAGTRALYAYYARYAVMARARGLGFVLESPTWRASRDWGEKLGYGAAALAEVNAKAI
                     RLMGELRAAFASAGSPMVISGCVGPRGDGYDPGHVMGAEEAGDYHSPQIASFAANGAD
                     LVTAMTMTNVPEAIGVTRAAAALRLPAVIAFTLETDGRLPTGQGLADAIAEVDGATGA
                     GPRYYMINCAHPTHFADVLEARSGWTRRIGGLRANASRRSHAELNEAPDLDAGDPSEL
                     GGQYRALLARHPQLKVLGGCCGTDHRHLEAICAACGLAPVETSRPLAPA"
     gene            complement(1089..1652)
                     /locus_tag="VFR00_15240"
     CDS             complement(1089..1652)
                     /locus_tag="VFR00_15240"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU0160676.1"
                     /translation="MRLMPGLDRRPTGPLPAGIEIRQLGVDDFPAVRYLHGTVLRAET
                     ADALTEGEIATFAQLVNSPAYSDLLREENEIFSAWLGGELLGTAAWRINADDGTQARI
                     GFVFARHPGFGIGSRLLAEAESRALAWGFDRLTSFTTLNAIGFFQKRGYGVVSRGVKV
                     FSPECALPVAFLSKRLSRRPGPPGRTH"
     gene            complement(1719..2849)
                     /locus_tag="VFR00_15245"
     CDS             complement(1719..2849)
                     /locus_tag="VFR00_15245"
                     /inference="COORDINATES: protein motif:HMM:NF022515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2865 domain-containing protein"
                     /protein_id="HEU0160677.1"
                     /translation="MLNGKQRLCSLRLILVGLAVLAALGVGLGDALAQGWNWWPFGGQ
                     SEPRPIPREPLYRGPPGQAPPGYPPGTPGQYPPGQYPPGQYPPGQPPPGQPYWGQRGA
                     NTNICLQLEQRLVAETQGSGQNREQLPKIETDLRQTERSLTTAQIQLDRADCWDQFLF
                     SKTLRRTPRCVQLNSDVENLRRRLAELDGQRRQILGTRDRSYQDDIIRELARNGCGPQ
                     YAQEARRRDAATNPFSALWSDEGDAPRGPGNQFGNLPFATYRTLCVRLCDGYYFPVSF
                     STLPNHFQRDAEVCQSRCAAPAELYYHQNPGGAVEQAISVATQQPYTSLKSAWRYRKE
                     FVQGCSCKEAEYVPAPGTGEKKADAAPVTPPPRSEARLLTPR"
     gene            3164..>4590
                     /gene="cysS"
                     /locus_tag="VFR00_15250"
     CDS             3164..>4590
                     /gene="cysS"
                     /locus_tag="VFR00_15250"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEU0160678.1"
                     /translation="MLTLYNTLTRTKERFEPIDPSNVRMYVCGPTVYDLAHIGNARPI
                     IVFDVLFRLLRHLYGAGQVTYARNITDIDDKINARAVELYPDLQPTEAIRRLTKETTE
                     QFHKDVAALGALPPSVEPRATDTIPEMRALIEALIARGHAYVAEEHVLFDVSSLPAYG
                     RLANRSLAEMEAGARVDVAPYKKGPMDFVLWKPSSAGVPGWDSPNGITSKGRPGWHIE
                     CSAMADKFLWELAKGTLTAKGLAQPHVFDIHGGGIDLVFPHHENEIAQSRGGHGSAVM
                     ANVWMHNGFLQVEGEKMSKSLGNFITIRDLLDAGWDGRVLYFAMLKTHYRQPINWTRK
                     GMVEAALELDSLRQHLVAPPYADAQSQEFLAALADDLNTPGAIAELHRLARASHAEAG
                     KAHAQTVEQARARLANALKLIGLDHLRDFDVHKVLARVTGRAPIDADAVLKLVAQRNA
                     ARKARDFRQADALRDQLWAMGVQLK"
BASE COUNT          784 a         1481 c         1592 g          733 t
ORIGIN      
        1 gggcctgcgc gcgccccgca agcaaaaagc attggagtga gccatgagcc tcaatcgatc
       61 gccgctgcca caagagggcg ggggcctgtt tctgaccgac ggtggtatcg aaaccacctt
      121 gatcttccat gagggttggg agctgccgca tttcgcggcc ttcgacctgt tgcagacgcc
      181 cgcagggacg cgcgcgcttt atgcctatta cgcgcgctac gccgtcatgg cgcgcgccag
      241 gggcctcggc tttgtgctgg aaagtccgac ctggcgggcc agccgcgact ggggcgaaaa
      301 gctcggctac ggcgccgccg cgctcgccga ggtcaacgcc aaggccattc gcctgatggg
      361 cgagctgcgc gcggccttcg ccagcgccgg cagccccatg gtgatcagcg gttgcgtcgg
      421 gccaaggggc gatggctatg atccgggcca cgtcatgggg gccgaggagg cgggcgacta
      481 tcatagcccg caaatcgcga gcttcgcggc caacggcgcc gacctggtga cggccatgac
      541 catgacgaac gtgcccgagg cgatcggcgt gacccgtgcg gcggcggcct tgaggctgcc
      601 ggctgtcatt gcctttacgc tcgagaccga cgggcggctg ccaaccgggc agggcctggc
      661 ggatgccatc gcggaagtcg acggggcgac gggagccggt ccccgctact acatgatcaa
      721 ctgcgcccat ccgacgcatt ttgccgatgt gctcgaggcg cgttcgggct ggacccggcg
      781 gattggcggc ctgcgcgcca acgcctcgcg gcgcagccac gccgagctca acgaggcacc
      841 cgatctcgac gctggggacc cgagcgagct gggcggccaa taccgcgcgc tcctggctcg
      901 gcacccgcag ctgaaggtgt tgggcggctg ctgcggcacg gaccatcgcc atctcgaagc
      961 gatctgcgcg gcctgcgggc ttgcaccggt cgagacctcc cggccgctgg cgccggcctg
     1021 aggcgagccc gctgcgcctc ggctgcggca aagacgcggg cccccaagtt ttgggcgcgc
     1081 ggcggcggct agtgggtgcg cccgggcggc ccggggcgtc ggctcaggcg cttggacagg
     1141 aaggcgacgg gcagcgcgca ttcgggggag aatactttga ccccgcgcga gacaacgccg
     1201 tagccgcgtt tttgaaaaaa gccaatggca ttcaaggtcg taaaggaggt gaggcggtcg
     1261 aaaccccagg ccagcgcgcg gctctccgcc tcggcgagca gacggctgcc gatgccgaaa
     1321 ccggggtggc gggcgaacac gaagccgatg cgtgcctggg tgccgtcgtc ggcgttgatg
     1381 cgccaggcgg ccgtgcccag gagctcgccg ccgagccagg cggagaagat ctcgttttct
     1441 tcccgcagca gatccgaata ggccggcgag ttgacgagct gcgcgaaggt cgcgatctcc
     1501 ccttccgtta gggcatcggc ggtttcggcg cgcaggacgg ttccgtgcag gtagcggacg
     1561 gccggaaagt cgtcgacccc cagctgtctg atttcgatgc cggcgggcaa aggaccggta
     1621 ggacggcggt cgaggcccgg cattagacgc atgaactctt gtgcggcatc tgttcggcaa
     1681 cacacccgtt gggggcgagg acgccgtcga gaccggccct agcggggggt gaggaggcgg
     1741 gcctccgacc ggggcggcgg ggtcaccggc gcggcatcgg ccttcttttc cccggtgccg
     1801 ggcgcgggca cgtattcggc ttccttgcag gagcagccct gcacgaactc tttgcgataa
     1861 cgccaggccg atttcaagct ggtatagggc tgctgggtgg caaccgagat cgcctgctcg
     1921 accgcgccac cggggttttg atggtagtaa agctcagccg gtgccgcgca gcgcgactgg
     1981 cagacctccg catcgcgctg gaaatggttg ggcagcgtgg aaaaactgac cgggaagtaa
     2041 tagccgtcgc agagccgcac gcacaacgtg cggtaggtgg cgaagggcag gttaccgaac
     2101 tggttgcccg ggccgcgcgg ggcgtcgccc tcatcggacc acagggcgga aaaggggttg
     2161 gtggcggcat cccgtcgccg ggcttcctgc gcgtattgcg ggccgcagcc gttgcgcgcg
     2221 agctcgcgaa taatgtcgtc ctgataggag cgatcgcgcg tgccgaggat ttgccggcgc
     2281 tggccatcga gctcggcgag ccggcgacgc aggttttcca catcgctatt gagctgcacg
     2341 cagcgcgggg tgcggcgcag cgttttggag aacaagaact gatcccagca atcggcccgg
     2401 tcgagctgga tctgtgccgt ggtgagactg cgttcggtct gacgcaggtc ggtttcgatc
     2461 ttgggcaatt gttcacggtt ttgcccgctg ccctgggtct cggcgaccag ccgctgctcg
     2521 agttgcaggc agatgttggt gttggccccg cgctggcccc aatagggctg gccgggaggg
     2581 ggctgacccg gcggatactg gccgggcgga tactggccgg gcggatactg gcccggcgtg
     2641 ccgggtggat aaccgggtgg ggcttgcccc ggcgggcccc ggtaaagcgg ctcgcgcggg
     2701 atcggccgcg gctcggattg ccctccgaac ggccaccagt tccagccctg ggccagggca
     2761 tcgccgaggc ccacgcccaa ggcggcaagg acggccaagc cgaccaagat aagccgcagg
     2821 ctgcacaatc tctgcttgcc gttcagcatt ggaaaattcc gaagtccccc gcgcgccccg
     2881 gcgccatcgc aagcgccgct cgacgccgaa tctggcgaaa cttcccctcc aggagattat
     2941 gggagtgttg ccctagcggg tcaatgcagc gcaaattggg cgctcaagtc ggcttgacgc
     3001 tgcctgggaa agcgcgggat aagcggccca tgccgacgct gtcgataccg gccgtcaata
     3061 ttccgtgcgg caatattcca acgagctagc cacaggctgg cgcggccgct tctttgccca
     3121 cccgtgacag agaaaacgcc ccgcccggcg gcaagggtga acgatgctca cgctatacaa
     3181 cacgttgaca cggaccaagg agcggttcga gcccatcgat ccgagcaacg tgcgcatgta
     3241 tgtgtgcggg cccacggttt acgacctcgc ccatatcggc aacgcccgcc cgatcatcgt
     3301 cttcgatgtg ctgtttcggc tgctgcggca cctctatggg gctgggcagg tgacctatgc
     3361 ccgcaacatc accgacatcg atgacaagat caacgcgcgc gccgtggagc tttacccgga
     3421 tctgcaaccg accgaggcca tccgccggct gacaaaagaa acgacggaac agttccacaa
     3481 ggatgtggcg gcgctcggcg ccctgccgcc cagcgtcgag ccgcgtgcaa ccgataccat
     3541 ccccgagatg cgcgccctga tcgaggcgct catcgcccgc ggacatgcct acgtggccga
     3601 ggagcacgtc ctgttcgatg tgtcctcgct ccccgcctac ggccgcttgg cgaaccggtc
     3661 cttggccgag atggaggcgg gggctcgcgt tgatgtggcg ccctataaga aagggccgat
     3721 ggacttcgtg ctctggaagc cgtcgtccgc cggcgtaccg ggatgggact cgcccaacgg
     3781 catcaccagc aagggacgcc cgggctggca catcgagtgc tcggcgatgg ccgacaaatt
     3841 tctctgggag ttggccaagg gcacgctgac cgccaaaggc ctggcgcagc cgcacgtgtt
     3901 cgacattcac ggcggcggta tcgacctggt cttcccccac catgaaaacg agatcgccca
     3961 gtcgcgtggc ggtcacggca gtgccgtgat ggccaacgtc tggatgcaca atggcttcct
     4021 gcaggtcgag ggtgagaaga tgtcgaaatc gctcggcaac ttcatcacca tccgagacct
     4081 gctggacgcg ggctgggacg gccgcgtcct ctattttgcg atgctgaaga cgcactatcg
     4141 ccagcccatc aactggacgc gcaagggcat ggtcgaggct gccctcgagc tcgacagcct
     4201 gcgtcagcac ttggtcgcgc cgccctatgc cgatgcgcag agccaagagt tccttgctgc
     4261 gctggccgac gacctcaaca cgcccggcgc gatcgcagag ctgcatcggc tggcgcgcgc
     4321 cagccacgcc gaagcgggca aggcgcacgc acaaaccgtc gagcaagcgc gcgcgcgcct
     4381 tgccaatgcg ctgaagctca tcggcctcga ccatctaagg gatttcgatg tgcacaaggt
     4441 gctggcacgg gtcacgggcc gggcccccat cgatgcggat gccgtgctga aactggtggc
     4501 acagcgcaac gccgcccgca aagcccggga tttccgtcag gccgacgcgc tgcgcgatca
     4561 gctctgggct atgggcgtgc agctcaagga
//
LOCUS       DASTMT010000660        11281 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_5812754, whole genome shotgun sequence.
ACCESSION   DASTMT010000660 DASTMT010000000
VERSION     DASTMT010000660.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 11281)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11281)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11281
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5812754"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            222..455
                     /locus_tag="VFR00_15255"
     CDS             222..455
                     /locus_tag="VFR00_15255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0160679.1"
                     /translation="MIHTCTKSALAALLILALATPAVAQSRASERERRPACSRTDSCA
                     PSLSAADRENLSRAMSRNVLHSIDDRLRRQRYN"
     gene            658..1428
                     /locus_tag="VFR00_15260"
     CDS             658..1428
                     /locus_tag="VFR00_15260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0160680.1"
                     /translation="MSVLAGCASLPPYRDDAVSTAEIARHIKCELREAAWSKPSHEWL
                     HTWNAALVLSLEVFHNGEVGADTAVVLPISHGGSFLSNLTGATSGQATRTERISFNES
                     LAALHRDPSLRCEERDTERYGRLGGRLGIADLFERAGRTRKLANLTGLTQLDYNLDFI
                     IKNNAGLEPRFVVVPIGHDRTIGGGLKLAGSNSDTQTLKLTLVPPAQGASTRGLATGP
                     RGAGAAIPSADEERLERSKSRNLLESIDDKLRRQNLGN"
     gene            1585..1950
                     /locus_tag="VFR00_15265"
     CDS             1585..1950
                     /locus_tag="VFR00_15265"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009450025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HEU0160681.1"
                     /translation="MNQLLIDYLPLAVFIGVALLIGAALMLAPFLVAVRNPDPEKVSA
                     YECGFNAFDDARMKFDVRFYLVSILFIIFDLEVAFLFPWAVAFKQVGLFGFWSMMVFL
                     AVLTIGFIYEWRKGALEWD"
     gene            1950..2501
                     /locus_tag="VFR00_15270"
     CDS             1950..2501
                     /locus_tag="VFR00_15270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004680048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HEU0160682.1"
                     /translation="MVSQAPSSHERRQSGLLTPATGSFGDVSAQLADKGFIVTTTDDL
                     INWARTGSLMWMTFGLACCAVEMMQASMPRWDLERFGFAPRASPRQSDVMIVAGTLCN
                     KMAPALRKVYDQMPEPRYVISMGSCANGGGYYHYSYSVVRGCDRIVPVDVYVPGCPPT
                     AEALVYGVLLLQRKIRRTGTIER"
     gene            2536..3129
                     /locus_tag="VFR00_15275"
     CDS             2536..3129
                     /locus_tag="VFR00_15275"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HEU0160683.1"
                     /translation="MLDQLAQHIAALLGANCLRQTLALGELSVVVPAEAIVSVLRTLR
                     DDPQCQFEQLIDICGVDYPEREKRFDVVYHLLSLRRNQRLRIKCEIDEKTPVPSVVEL
                     FVVANWYEREAYDMYGILFSGHPDLRRILTDYGFQGFPLRKDFPLTGYVEVRYDDAQK
                     RVVYEPVRLPQEFRSFDFMSPWEGTEYVLPGDEKAKP"
     gene            3132..4322
                     /locus_tag="VFR00_15280"
     CDS             3132..4322
                     /locus_tag="VFR00_15280"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011424925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HEU0160684.1"
                     /translation="MPETDIRQFNINFGPQHPAAHGVLRLVLELDGEVVTRVDPHIGL
                     LHRGTEKLIEHKTYLQAIGYFDRLDYVAPMNQEHAFCLAGEKLLEIAVPKRAQLIRVL
                     YCEIGRLLSHLLNVTTQAMDVGALTPPLWGFEEREKLMVFYERASGSRMHAKYFRFGG
                     VHQDLPPRLIEDIAKFCETHPKVLDDIEDLLTDNRIFKQRNVDIGVVKLEDCVRWGFS
                     GVMLRGSGAAWDLRKAQPYECYSELDFDIPIGKNGDNYDRYCIRVEEMRQAVRIMRQC
                     CEKLMSAEGAGPHVSADNKVIPPKRAEMKRSMEALIHHFKLYTEGFHVPAGEVYAAVE
                     APKGEFGVYLVSDGSNRPYRCKIRAPGFPHLQAMDFLNRGHMLADVSAILGSLDIVFG
                     EIDR"
     gene            4368..4769
                     /locus_tag="VFR00_15285"
     CDS             4368..4769
                     /locus_tag="VFR00_15285"
                     /inference="COORDINATES: protein motif:HMM:NF025453.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4267 domain-containing protein"
                     /protein_id="HEU0160685.1"
                     /translation="MSAARSRKTTLFLLAAGFVAGTLLGVIGLRYFLVPQSAARSFGV
                     AGRPAGHELYYIIGLRNLWLATLAIGLAALRQWHGLALWFAAGTVVCFADALIAANAT
                     GKVPQVAFHLVCGLGCALLATIAWRRAGKGP"
     gene            4898..5515
                     /gene="nuoE"
                     /locus_tag="VFR00_15290"
     CDS             4898..5515
                     /gene="nuoE"
                     /locus_tag="VFR00_15290"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006611008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HEU0160686.1"
                     /translation="MSVRRLDPQQPPSFAFTPENEAWAKAMIAKYPPGKQASAVIALL
                     WRAQEQQAGWLPEPAIRLVADMLGMAHIRALEVATFYTMFQLSPVGKRAHVQVCGTTP
                     CMLRGSQALVEVCRRRINADPHHLSADGDFSWEEVECLGACANAPMVQIWKDTYEDLT
                     PELFEKVLDGFASGTAPKAGSQIGRQASCPAGGPTTLLDLAKAGK"
     gene            5531..6817
                     /gene="nuoF"
                     /locus_tag="VFR00_15295"
     CDS             5531..6817
                     /gene="nuoF"
                     /locus_tag="VFR00_15295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HEU0160687.1"
                     /translation="MALQDKDRIFRNLYGFHDWKLAGAKARGAWDGTTALIEKGHDAI
                     IAEIKNSGLRGRGGAGFPTGLKWSFMPKNDPRPSYLVINADESEPGSCKDREIMRHDP
                     HLLVEGALLAAFAMRAHSCYTYVRGEFVRERERLEAAVAEAYEAKLIGKNNVHGWDFD
                     FIVHHGAGAYICGEETALLESLEGKKGLPRLKPPFPANTGLYGAPTTVNNVESIAVVP
                     DILRRGATWFAGFGKPNNTGTKLFQISGHVEQPCVVEEEMGVDLRGLLERHAGGVRGG
                     WDNLLAVIPGGSSMPCLPAHLCQDLTLDFDTLSKLKSGMGTAAVIVMDKSTDIIAAIR
                     RIAYFYKHESCGQCTPCREGCGWMWRMLRRMELGEARKSEIDLLQDMTTKIEGHTICA
                     LGDGAAWPVQGLLRHFRGVIEERIDRHQAALKQAAE"
     gene            6821..8932
                     /gene="nuoG"
                     /locus_tag="VFR00_15300"
     CDS             6821..8932
                     /gene="nuoG"
                     /locus_tag="VFR00_15300"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HEU0160688.1"
                     /translation="MPKQLIIDGTPIEVEDGITLIQACELAGVEIPRFCYHERLSIAG
                     NCRMCLVEVQGMPKPAASCAMGVNDLPPNKDGSPKIINTKSPMVKKAREGVMEFLLIN
                     HPLDCPICDQGGECDLQDQAMAYGMGGSRYVENKRAVDEKYLGPLIKTFMTRCIQCTR
                     CVRYMTEVAGVEELGAIGRGEDMEITTYLERGMMSELSANVVDLCPVGALTHRPWAFV
                     ARPWELRKTESVDVMDAVGSAIRVDCRGREVVRILPRNNDAVNEEWISDKTRHVADGL
                     RIQRLDQPYLRHGGALKPAGWDEVLALVGGKLKSTPPERIGAIAGDLAGAEEMFALKD
                     LFGRLRCRNLDCRQDGAKLDPALGRASYLFNSSIEGIERADAILIIGANPRLEAAVLN
                     ARIRKRWRQGNLAVGLIGEAADLTYPYDYLGAGPQTLKEVADGKHSFAGVLAKAKQPM
                     IIIGASAAARADGVGVLAAAAAIALRAADGKEPGWNAFNLLNTAASRVAGLDLGFVPA
                     DGGLDVAGMLLGAAGGTLEVLYLLGADEIDMSAIGEEAFVIYQGSHGDAGAHRADVVL
                     PGAAYTEKAATYVNLEGRAQMTNRAAFAPGEAKEDWAIIRALSAHTGHTLPYDRPQAL
                     RAAMAKLAPQLMRLEGLAAADLAGVTALAGRKGPLAADPFVASTRDFYLSNPIARASS
                     IMAELSALKKSWDKDTTGTHG"
     gene            8925..10001
                     /gene="nuoH"
                     /locus_tag="VFR00_15305"
     CDS             8925..10001
                     /gene="nuoH"
                     /locus_tag="VFR00_15305"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HEU0160689.1"
                     /translation="MASGWLWSEIWQAYLWPLLRMLLESLLLLVALLITIAFLLLMDR
                     KVWAAVQLRRGPNVVGPFGLLQSFADLLKFVLKEPLIPAGADKGVFLLAPLVASVLAL
                     AAWAVIPFNENSWGRWVISDINVGMLYLFAISSLGVYGIIMGGWASNSKYPFLGALRS
                     AAQMVSYEVSIGFVIITVLLVVGSLNLTEIVNAQKVGIGTRLGLVNSLLDWNWLPLFP
                     MFIVFFISALAETNRPPFDLPEAESELVAGFAVEYSSAPYLLFMLGEYVAIVTMCALT
                     TIMFLGGWLPPLDLAPLNFVPGLVWFLLKVVFVFFMFAMVKAFVPRYRYDQLMRLGWK
                     VFLPLSLFMVVAVAAVLQYFELAP"
     gene            10012..10503
                     /gene="nuoI"
                     /locus_tag="VFR00_15310"
     CDS             10012..10503
                     /gene="nuoI"
                     /locus_tag="VFR00_15310"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HEU0160690.1"
                     /translation="MAGIGQAVNALFLKEFASALWLSLRYFFKPKATLNYPFEKGPLS
                     PRFRGEHALRRYPNGEERCIACKLCEAICPAQAITIEAGPRRNDGTRRTTRYDIDMVK
                     CIYCGLCQEACPVDAIVEGPNFEFATETREELYYDKERLLANGDRWEREIAKAIELDA
                     PFR"
     gene            10511..11158
                     /locus_tag="VFR00_15315"
     CDS             10511..11158
                     /locus_tag="VFR00_15315"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HEU0160691.1"
                     /translation="MLAAAFFYLFAAMTVASGAMVIVARNPVHAVLFLILAFFNAAGL
                     FVLLGAEFLAMILVVVYVGAVAVLFLFVVMMLDVDFAELRAGFINNAPLGAMVGLVLL
                     AELIFVLGVHIIDPRALAAPAAPIPTTGSGITNTEAIGRVLYTKYVYYFQGAGLILLV
                     AMIGAIVLTLRHKEGVKRQSIARQVARRKATAMDIVKVKPGDAAIPEVAVVDERG"
     gene            11170..>11281
                     /locus_tag="VFR00_15320"
     CDS             11170..>11281
                     /locus_tag="VFR00_15320"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010066755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit K"
                     /protein_id="HEU0160692.1"
                     /translation="MAIQLGHYLTVAAILFTLGIFGIFLNRKNVIVILMSI"
BASE COUNT         1912 a         3692 c         3599 g         2078 t
ORIGIN      
        1 aaaaaggcaa ccgcagtcag ggcgagctga cgcatgggat gggctccttg catgaccttc
       61 ctggcgggta aaccctgaac gcgcccagaa ggttgcgcgc caaggtggga cggccgcgtg
      121 ctccccgcag aaacctcgca atctccccct gctggtctcg acaccttgga aaagtccaga
      181 tcgtttgcca ttttcacaag cacgagagga gcgcgacggc catgattcac acgtgcacca
      241 aatcggcgct ggccgccctg ctgatcctcg cgctggcgac acccgcggtg gcccaaagcc
      301 gcgccagcga gcgcgaaagg cgccctgcgt gcagtcgaac cgacagctgt gcgccgagcc
      361 tctctgccgc cgatcgcgag aacctatcgc gtgccatgag ccgcaacgtc ctgcacagca
      421 tcgacgatcg cttgcgccgt caacgctata actagcggct acacaacgac atctcctgac
      481 gggctggccc ctccggcgca gggctcggcg ttgttgaaaa acaacagcga gagcttctaa
      541 gccgcccgcc gcagcgatcg agcttgtgag gcaccacccc acccgtcaca gtcatttttt
      601 tcgtgctgcg ttgggggcag tatgcgtagc ttgggcgcgc gagcggggtg cgtagcgttg
      661 tctgtgctcg ccggctgtgc cagccttccg ccctatcgcg acgatgccgt cagcaccgcc
      721 gagattgcgc gccacatcaa atgcgagctg cgcgaggcgg cctggtccaa gcccagtcac
      781 gaatggctgc atacgtggaa tgccgccctc gtgctcagcc tcgaggtctt tcacaatggc
      841 gaggtcggcg ccgataccgc cgtggtcttg cccatttcgc atgggggctc attcctttcc
      901 aacctgaccg gggcgacgag cgggcaggca acccgcaccg agcggatcag ctttaacgaa
      961 agtctggccg cgctgcaccg cgatcccagc ctgcgctgtg aggagcgcga cacggaacgc
     1021 tacggccggc tcgggggtcg attgggtatc gccgacctct tcgaacgcgc cggccgaacc
     1081 cgcaagcttg ccaatctgac gggactgacg cagctcgact acaacctgga tttcattatt
     1141 aagaacaatg cggggctgga gccccgcttt gtcgtggtgc cgatcggcca cgaccgaacc
     1201 attgggggcg ggctcaaact cgccggctcc aacagcgaca cccaaacgct caagctcaca
     1261 ctggtgcccc cggcgcaggg agcctcgacc cgcggcttgg caaccggccc gcgtggggcc
     1321 ggcgcagcga tcccctctgc ggacgaggag cgcttggaac gcagcaagag ccgcaacctg
     1381 ctcgaaagca tcgacgacaa gctgcgtcgg cagaacctcg gcaattgagg agcccgtccc
     1441 cggggcaacg aaggccccct ggccagcacc gcgggccgcc agccgcccgt tgcgacgaag
     1501 gcgccggttg cgtcaccggt tgccctgcgg taacacccgg cctgcagcca cccccggata
     1561 gccgcaagcg gagccaggcg gcgaatgaac caactcctga tcgactacct gccgctcgcc
     1621 gtctttatcg gcgtcgccct cctcatcggg gcggccttga tgttggcgcc gtttctggtg
     1681 gccgtgcgta atcctgatcc ggaaaaggta tctgcctacg agtgcggctt taatgccttc
     1741 gacgacgcgc gcatgaagtt cgacgtgcgt ttctacctgg tctcgattct gttcattatt
     1801 ttcgacctgg aagttgcctt cctgttcccg tgggccgtcg ccttcaagca ggttggcctg
     1861 ttcgggttct ggtcgatgat ggtcttcctc gccgtattga ccatcggctt catctacgag
     1921 tggcgcaagg gtgcgctgga gtgggattga tggtgagcca ggccccgagc agtcacgaac
     1981 gccgccagtc cgggctcctt accccggcta ccggctcgtt cggcgacgtc tcagcccagc
     2041 tcgccgacaa gggctttatc gtcaccacca cggatgacct catcaactgg gcgcgtacgg
     2101 gctcgctgat gtggatgaca ttcggacttg cctgctgcgc cgtcgagatg atgcaggcct
     2161 cgatgccgcg ctgggatctc gagcgcttcg gctttgcgcc ccgcgcctcg ccgcggcagt
     2221 ccgacgtgat gattgtcgcg ggcacgctgt gcaacaagat ggctcccgca ctgcgcaagg
     2281 tctacgacca gatgccggag ccgcgctacg tcatctccat ggggagctgt gccaacggcg
     2341 ggggctatta ccactactcc tattccgtgg tgcgcggctg cgaccgcatc gtgccggtcg
     2401 acgtttatgt gccgggctgc ccgccgaccg cggaagcgtt ggtctacggc gtgctgttgt
     2461 tgcaacgcaa gatccggcgc actggaacga tcgaacgcta accgcccgcc gaacctcaag
     2521 agcaagcgct cgcccatgct cgatcaactt gcccagcaca ttgccgctct cctgggcgcc
     2581 aactgcctgc ggcagaccct ggcgttgggc gagctaagcg tggttgtgcc ggccgaggcg
     2641 atcgtctcgg ttttgcgcac cttgcgggac gacccgcagt gccagttcga gcagctcatc
     2701 gacatctgcg gcgtggacta tcccgagcgg gagaaacgct tcgacgtcgt ctaccacctg
     2761 ctgtcgctac gccgaaacca acgcctgcgc atcaaatgcg agatcgacga aaaaacgccc
     2821 gtacccagcg tggttgagct ctttgtcgtg gccaattggt acgagcggga agcctatgac
     2881 atgtatggca tcctgttctc cgggcatccg gacctgcgcc ggatcctcac cgactacggc
     2941 ttccagggct tcccgctgcg caaggatttc ccgcttacgg gctacgtcga ggtccgctac
     3001 gacgatgcgc aaaagcgcgt tgtttacgag ccggtgcggc tgccgcagga gtttcgttcc
     3061 ttcgacttca tgagcccgtg ggaggggacc gagtacgtgc tccccggcga cgaaaaggca
     3121 aagccgtagc aatgccagag acggatatcc gccagttcaa catcaatttc ggtccgcaac
     3181 atccggccgc ccatggcgtg ctgcgcctcg tgctcgaact cgacggggag gtcgttaccc
     3241 gcgtcgaccc ccacatcggc ctgttgcacc gcggcacgga aaagttgatc gaacacaaga
     3301 cctatctgca ggccatcggc tatttcgatc gcctcgacta cgtggcgccg atgaatcaag
     3361 agcacgcctt ctgcctcgcc ggcgagaagc tgctcgagat cgcggtgccc aagcgggcgc
     3421 agctgatccg cgtgctttat tgcgagatag gtcggctgct ctcccacctt ctcaacgtca
     3481 cgacgcaggc catggatgtc ggcgctttga ccccgccgct gtggggcttc gaagagcgcg
     3541 agaagctcat ggtattttac gagcgcgcct cgggcagccg gatgcacgcc aaatattttc
     3601 gcttcggcgg cgtgcaccag gatctgccgc cacgcctgat cgaagacatc gccaagttct
     3661 gcgagacgca ccccaaggtg ctggacgaca tcgaggacct cctcaccgac aatcgcattt
     3721 tcaagcagcg caacgtcgac attggcgtcg tcaagctcga ggattgcgtc cgctggggct
     3781 tctccggcgt gatgttgcgc ggctccggcg ccgcctggga cctgcgcaag gcccagccct
     3841 acgagtgcta ttccgagctc gatttcgaca tcccgatcgg caagaacggc gacaactacg
     3901 atcgctactg cattcgcgtg gaggagatgc gtcaggctgt gcgcatcatg cgccaatgct
     3961 gcgagaaact gatgtctgcc gagggtgcgg gtcctcacgt cagcgccgac aacaaggtga
     4021 tcccgcccaa gcgggccgag atgaagcgtt cgatggaagc gctgatccac cacttcaagc
     4081 tttataccga gggcttccac gttcccgccg gcgaggtcta tgccgccgtc gaggcgccca
     4141 agggcgagtt cggggtctat ctagtgtcgg atggcagcaa ccgcccctac cgttgcaaga
     4201 tccgcgcgcc cggcttcccg catctgcagg ccatggactt tttgaaccgg gggcacatgc
     4261 tggccgacgt ctcggccatc cttggctccc tcgatatcgt cttcggcgag atcgaccgtt
     4321 aaccctgcgg gccggcggcc cgtcgcagcc ggacggggcg ctttgacgtg agcgcggcca
     4381 gatcaagaaa gaccaccctc ttcctcctcg ccgccggctt tgttgccggc accctcctcg
     4441 gcgtcatcgg cctgcgctat ttcctggtcc ctcagtcggc cgcgcgcagc ttcggcgtcg
     4501 cgggccggcc ggctgggcat gagctctact acatcatcgg cctgcgcaat ctctggctgg
     4561 ctacgctcgc gatcggtctg gctgcgctgc gccagtggca tggcttggcg ctgtggtttg
     4621 ccgccggcac cgtcgtctgt ttcgccgacg cgctgattgc cgccaatgcc acgggaaagg
     4681 tgccgcaggt ggccttccac ctcgtctgcg ggctcggctg tgccctcctg gccaccatcg
     4741 cttggcgccg cgccggcaag ggcccctgag cgacaagcaa ctttgccgcg ctcgatcccc
     4801 ttggcatcgt tggaaagacg ggcgacaagc gccaaatcag cccttataag ggggccgccg
     4861 caatttcgcc tccgccgttc ggagcacgcc gagcccaatg tctgtccgca gacttgaccc
     4921 tcaacaaccc cccagcttcg ccttcacgcc cgagaacgag gcgtgggcca aggccatgat
     4981 cgccaagtat ccgccgggca agcaggcctc ggccgtcatc gccctgctct ggcgggcgca
     5041 ggagcagcag gcgggctggc tgccggagcc cgccatccgc ctggtcgccg acatgctcgg
     5101 catggcccac attcgcgccc ttgaggtggc gaccttctac accatgttcc agctctcccc
     5161 ggtgggaaag cgggcgcacg tgcaggtgtg cggtaccacc ccgtgcatgc tgcggggctc
     5221 gcaagccctg gtggaggtct gccggcggcg cataaatgcc gatccacacc acctctccgc
     5281 cgacggggat ttctcctggg aggaggtcga gtgccttggc gcctgcgcca atgcgcccat
     5341 ggtgcagatt tggaaggaca cctatgagga cctgacaccg gagctgttcg agaaggtatt
     5401 ggacggcttt gccagcggca cagctcccaa ggctggctcg cagattggcc ggcaggcatc
     5461 gtgcccggca ggcgggccaa cgacgcttct ggacttggcc aaggctggaa agtaggggac
     5521 ctaccgcgtc gtggctctac aggacaagga ccggattttc cgcaatctct acggcttcca
     5581 cgactggaag ctagccggcg ccaaggcccg cggtgcctgg gacggcacca cggcgctgat
     5641 cgaaaagggc cacgacgcca tcatcgccga aatcaagaat tcgggcctgc gcggccgcgg
     5701 cggggcgggc tttccgaccg gcctcaagtg gtccttcatg cccaagaacg atccgcgccc
     5761 cagctacctc gtgatcaatg ccgacgagtc cgagccgggc tcgtgcaagg atcgcgagat
     5821 catgcgccac gatccgcacc tgttggtcga gggcgccctg ctggcagcct tcgcgatgcg
     5881 ggcgcatagc tgttacacct acgtgcgcgg cgagttcgtg cgtgagcgcg agcggctgga
     5941 ggcggccgtg gcggaggcct acgaggccaa gctgatcggc aagaacaatg tgcacggctg
     6001 ggacttcgac ttcatcgtgc accacggcgc cggcgcctac atctgcggcg aggaaacggc
     6061 gctattggaa agcctcgagg gcaagaaggg cttgccccgc ctgaagccac ccttccccgc
     6121 caacaccggc ctctacggcg cccccaccac cgtcaacaac gtcgaatcga tcgcggtggt
     6181 gcccgacatc ctgcgccgcg gcgcgacctg gtttgccggc ttcggcaagc ccaacaacac
     6241 cggcaccaag ctgtttcaga tctcgggcca cgtcgagcaa ccctgcgtgg tcgaagaaga
     6301 gatgggcgtc gacctgcgtg gattgctcga gcgccatgcc ggcggcgtgc gcggcggttg
     6361 ggacaacctg ttggccgtca tccccggggg ctcctcgatg ccgtgcctgc cggcccacct
     6421 ctgccaggac ttgacgctcg atttcgacac cttgtccaag ctcaagtccg gcatgggcac
     6481 ggcggccgtg atcgtgatgg acaagtcgac cgacatcatc gccgccatcc gccgcattgc
     6541 ctacttctac aagcacgaga gctgcgggca gtgcacgccc tgccgagaag gttgcggctg
     6601 gatgtggcgg atgctgcgcc gcatggagct cggggaggcg cgcaagagcg agatcgatct
     6661 gttgcaggac atgaccacca agattgaggg ccacacgatc tgcgcgctgg gcgacggcgc
     6721 cgcctggccg gtgcagggcc tgctgcggca cttccgcggc gtgatcgagg aacgcatcga
     6781 ccggcaccag gccgcgctga agcaggcggc ggagtgagca atgccgaagc aactcatcat
     6841 tgacggcacg ccgatcgagg tcgaggacgg catcaccttg atccaagcct gcgagctggc
     6901 gggcgtggaa atcccccgct tctgctacca tgagcgcctg tcgatcgccg gtaattgccg
     6961 catgtgcctg gtcgaggtgc agggtatgcc gaaaccggcg gcctcctgcg ccatgggggt
     7021 caacgatctg ccgcccaaca aggacggcag ccccaagatc atcaacacca aatcgccgat
     7081 ggtgaagaag gcgcgtgaag gcgtcatgga gtttctgctc atcaaccatc cgctcgactg
     7141 cccgatctgc gaccagggtg gcgagtgcga cctgcaggac caggcgatgg cctacggcat
     7201 gggaggctcg cgctacgtcg agaacaagcg cgccgtcgat gagaagtacc tggggccact
     7261 gatcaagacc ttcatgacgc gctgcattca atgcacgcgc tgcgtgcgct acatgaccga
     7321 agtggccggg gtggaggaat tgggcgcgat tggccgcggc gaggacatgg agatcaccac
     7381 ctacctcgaa cgcggcatga tgtcggagct gtcggccaac gtcgtcgatc tgtgcccggt
     7441 cggcgcgctg acgcatcggc cgtgggcctt cgtggcccgg ccctgggaac tgcgcaaaac
     7501 cgaaagcgtc gacgtcatgg acgcggtggg ctcggccatc cgggtggact gccgcggccg
     7561 cgaggtggtg cgcattttgc cgcgcaacaa cgacgcggtg aacgaggaat ggatatccga
     7621 caagacccgg cacgtggccg acggcttgcg catccagcgc ctggaccagc cctacctgcg
     7681 ccacggcggc gccctcaagc ccgccggctg ggacgaggta ctggcgctgg ttggcggcaa
     7741 gcttaagagc acgccgcccg aacgcattgg cgccatcgcc ggcgacctgg cgggcgccga
     7801 ggaaatgttt gccctcaagg acctgtttgg tcgcttgcgc tgccgcaacc tggattgtcg
     7861 ccaggacggc gccaaactcg atcccgcgct cgggcgcgcc agctatctgt tcaattcgag
     7921 catcgagggc atcgagcggg ccgacgccat cctcataatc ggcgccaacc cgcggctcga
     7981 ggccgccgtg ctcaatgcgc gtatccgcaa acgctggcgg cagggcaacc ttgcggttgg
     8041 cctgatcggc gaggcggcgg acctgaccta tccttacgac tacctcggcg ctgggccgca
     8101 gacgctcaag gaggtcgccg acggcaagca cagcttcgcc ggcgtgctgg ccaaggccaa
     8161 gcagccgatg atcatcatcg gggccagcgc ggcggcgcgc gccgacgggg tcggcgtgct
     8221 ggcggcggcg gcggcaatcg ccttgcgggc cgcggacggc aaggagcccg gctggaacgc
     8281 cttcaacctg ctcaatacgg cggcctcgcg cgtggcgggt ctcgatcttg gcttcgtgcc
     8341 ggccgatggc ggcctcgatg tcgccggcat gctgttgggc gccgccgggg gaacgcttga
     8401 ggtgctctat ctgctggggg ccgacgagat cgacatgtcg gcgatcggcg aggaggcctt
     8461 cgtcatctat cagggcagtc acggtgacgc cggtgcccac cgcgccgatg tggtgctgcc
     8521 gggcgcggcc tacacggaaa aggctgccac ctacgtcaac ctcgaaggcc gggcgcagat
     8581 gacgaaccgg gccgccttcg cgccgggcga ggcgaaggag gattgggcga tcatccgcgc
     8641 cctctccgcg cacaccggcc acacgctgcc ctacgaccgg ccgcaggccc tgcgcgcggc
     8701 gatggcaaag ctggcaccgc agcttatgcg cttggagggc ttagctgcgg ccgacttggc
     8761 cggcgtcacc gccctggcgg ggcgcaaagg tccgctcgcg gccgatcctt tcgttgccag
     8821 cacgcgcgac ttttatctca gcaacccgat cgcgcgtgcc tcaagcatca tggccgagct
     8881 cagcgccttg aagaagagtt gggataaaga caccacgggg acgcatggct agcggctggc
     8941 tctggagcga gatctggcag gcctatttgt ggccgctgct gcgcatgctg ttggaaagct
     9001 tgctgctgct ggtggcgctg ctcatcacca ttgccttttt gctgctgatg gaccgcaagg
     9061 tctgggcggc ggtgcagctg cgccgcggtc ccaacgtggt gggcccgttc ggcctgctgc
     9121 agtcgttcgc cgacctgttg aagtttgtgc tgaaggagcc gttgatcccg gccggcgccg
     9181 acaagggcgt gttcttattg gcaccgctgg tggcctccgt gctggcgctg gcggcctggg
     9241 cggtcatccc gttcaacgag aacagttggg gtcgctgggt catttccgac atcaacgtcg
     9301 gcatgctcta tctgttcgcc atttcctccc tcggcgtcta cggcatcatc atgggcggct
     9361 gggcgtcgaa ctcaaaatat ccctttcttg gcgcgcttcg ctcggcggcg caaatggtgt
     9421 cctacgaggt ctcgatcggc ttcgtgatca tcaccgtgct gttggtggtg ggctcgctca
     9481 atctgaccga gatcgtcaat gcccagaagg tcgggatcgg tacccggctc ggattggtga
     9541 actcgctgtt ggactggaat tggctgccgc tgtttccgat gttcatcgtc ttcttcattt
     9601 cggcgctggc ggaaaccaac cgcccgccgt tcgatctgcc ggaggcggaa tcggagctgg
     9661 tggcggggtt tgccgtcgag tattcctccg cgccctacct gctcttcatg ctcggcgagt
     9721 acgtcgccat cgtcaccatg tgcgcactga ccacgatcat gttcctgggc ggctggttgc
     9781 cgccgctcga cctggcgccg ctcaattttg tgccgggact ggtttggttc ctgctcaagg
     9841 tcgtattcgt cttcttcatg ttcgccatgg tgaaggcgtt cgtgcctcgc taccgctatg
     9901 accagctcat gcgcctgggc tggaaagtgt tcctgccgct gtcactgttt atggtggttg
     9961 cggtcgccgc cgtgctccag tacttcgaac ttgcgccgta agagaaagcc catggctggt
    10021 atcggacaag ccgtcaatgc gctgttcttg aaggagttcg cctcggcctt gtggctgtcg
    10081 ctgcgctact tcttcaagcc gaaggccacg ctcaactacc ccttcgagaa aggccccctg
    10141 tcgccgcgct tccggggtga gcacgcgctg cgtcgctatc ccaacggcga ggagcgctgc
    10201 attgcctgca agctctgcga ggcgatttgc ccggcgcagg ccatcaccat cgaggcggga
    10261 ccgcgccgca acgatggcac gcggcggacc acgcgctacg atattgacat ggtcaagtgc
    10321 atctactgcg ggctgtgcca ggaggcctgc ccggtcgatg ccatcgtcga gggaccgaac
    10381 ttcgagttcg ccaccgagac gcgcgaggag ctctactacg ataaggagcg cctgctcgcc
    10441 aacggcgacc gctgggagcg cgagatcgcc aaggccatcg aactcgacgc cccgttcagg
    10501 tgaggtggcg atgctagcgg cggccttctt ttacttgttt gcggccatga ccgtggcctc
    10561 gggcgccatg gtgatcgtgg cgcgcaaccc cgtgcacgcc gtgctgttct tgatcttggc
    10621 gttcttcaac gccgccgggc tgttcgtgct gctcggggcc gagttcctgg ccatgatcct
    10681 ggtggtggtc tacgtcggcg cggtggccgt gctgttcctg ttcgtggtga tgatgctcga
    10741 cgtcgacttc gccgaactgc gcgccggttt catcaacaat gcgccgctcg gagccatggt
    10801 cggtctcgtg cttttggccg agctgatctt cgtgctcggc gtccacatca tcgatccccg
    10861 cgcgctggcc gctcccgccg cgccgatccc cacgaccggc tcgggtatca ccaacaccga
    10921 agcgatcggg cgcgtgctct acaccaaata tgtctactat tttcaaggcg cggggctgat
    10981 cctgttggtc gccatgatcg gcgccatcgt gctgacgctg cgccacaaag agggcgtaaa
    11041 acgccagtcg atcgcccggc aagtggcgcg ccggaaggcg acggcaatgg acatcgtcaa
    11101 ggtcaaaccc ggggatgccg ccatcccaga ggtggccgtt gtcgatgagc gcggttgagg
    11161 catagcgcca tggccattca gcttgggcac tacctgacgg ttgccgcgat cctgttcacg
    11221 ctcggcatat ttggcatctt cctcaaccgc aagaacgtga tcgtcatttt gatgtcgatc
    11281 g
//
LOCUS       DASTMT010000661         5734 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_5239210, whole genome shotgun sequence.
ACCESSION   DASTMT010000661 DASTMT010000000
VERSION     DASTMT010000661.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 5734)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5734)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5734
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5239210"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..323)
                     /locus_tag="VFR00_15325"
     CDS             complement(<1..323)
                     /locus_tag="VFR00_15325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009028806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU0160693.1"
                     /translation="MIDHVSIAVRDLSGAAQFYARVLAPLGYAKLVERPANVGFGKRY
                     PEFWLNLRADLAPAAADTGSHVCLRAPSQEAVRGFHAEALAHGGRSDGDPGPRQAAMT
                     TYYGA"
     gene            complement(350..2614)
                     /locus_tag="VFR00_15330"
     CDS             complement(350..2614)
                     /locus_tag="VFR00_15330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010910804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEU0160694.1"
                     /translation="MTANGSTNNKWIGKRTIRPDGVEKVTGRAAFGADFSQPGMLYGK
                     VLRSPHPHARIKAIKFDKALALPGVKAVMSGRDLVDFPWDKPVILGIQDLRFISRNVM
                     ARDRAFYAGHAVAAVAATSASIAAAAVKLIEVDYEVLPWVIDVDEAIEPGAPILHEAM
                     RGVGDKPGVATTSNIAGKLEHKLGDVDAGFAAADVIIERSFKTKPVHQGYIEPHACLV
                     SVTKDGRANIWSSSQGHFMVRDMTGQLTGTKLSDIRAVPAEIGGGFGGKTIVYLEPLA
                     LVLARKSGRPVKMVMTREEVFRATGPTSGSSSTVKIGASKDGKITAVKATYRLQAGAF
                     PGSPIRGAVGCALSPYDIANVEISGFDVVTNRPKVAAYRAPGAPIGAFSVECVLDELA
                     TALGVDPLLLRQKNAAKQGTKAAYGPVFPRIGYQETLEAALGHPHYKAPLGPNQGRGV
                     ASGFWFNAGGESSATVNVNEDGTVVVVTGHPDIGGSRASTVNIVAEMLGIDYRQVQAL
                     IGDTSVIGFSALTGGSRVTFASAMVVTRATEEVIESLRARAAKLWKIDIEAVIWENGA
                     ARPAGANAGEFAPLTLAQLAAKASATGGPINGSSSLNTEGATGGFATHICDVEIDPET
                     GKLRIVRYTAFQDVGRAIHPSYVEGQIQGGVAQGVGWAINEECIFAANGLLDNPSFLD
                     YRMPVASDLPMIEPVLIEVPNDRHPQGVRGVGEVPIVPPLAAVANAVRHATGLRLTEL
                     PLSPPKVLAALSAK"
     gene            complement(2823..3776)
                     /locus_tag="VFR00_15335"
     CDS             complement(2823..3776)
                     /locus_tag="VFR00_15335"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018388711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEU0160695.1"
                     /translation="MQSFERYDDLPAAVRGSVLTIGNFDGVHRGHQALLGQVRLLARE
                     LGRPAAALLFEPHPRAIFQPDKPHFTLTPLPRKLLLLERFGLDVAFVLPFDAAFAALG
                     ADAFVERVLVAGLGVHHVVIGYDFRFGAKRTGDPEALRRAGAVHGFGVSVVSQIGEAG
                     EVFSSSAIRAELAQGDVGGAAEMMGHYWRVSGRVVGGAGRGTGLGFPTANLKLPREVA
                     LGHGIYAVRIYFEGLRFNAAAYLGTRPTFDAGEPVLELFLFDFEGNLYGREIEVEFIA
                     FVRPDAKFAAVDALKDQMRQDCTRAQALLQIAPQTPRISHP"
     gene            complement(3927..4379)
                     /locus_tag="VFR00_15340"
     CDS             complement(3927..4379)
                     /locus_tag="VFR00_15340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020090850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HEU0160696.1"
                     /translation="MNVQTRPGYHFEDLELGMEASFAKAVSAADVLSFAEVTGDRNPV
                     HLDAAYAAKTIFKEPIAHGMLTAGYISAVFGIEMPGPGAIYVSQTLNFRAPVRIGDRV
                     IAKVRVVELYPAKRRARFECTCSVDGKVVLEGEAVLMVPTRAAEQKAS"
     gene            4703..5461
                     /locus_tag="VFR00_15345"
     CDS             4703..5461
                     /locus_tag="VFR00_15345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012111008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell cycle transcriptional regulator TrcR"
                     /protein_id="HEU0160697.1"
                     /translation="MSDRKPLMPKATAVWLVENTSLSFEQIAEFCGLHALEVKGIADG
                     DVAHGIKGMDPISNGQLSREEIRRGEEDHGHVLRLAVSKVEIPEVKTKKGPRYTPVSR
                     RQDRPNAVIWLLRNHPELKDSQIMRLVGTTKPTIAAIRDRTHWNSANLTPQDPVTLGL
                     CSQIDLDNEVKKAARRVEREKKEQQKPAEKGGSLLPTSETTVVPGLGPQPDVEIVPPE
                     HAEPTREETEAEEQARVLAKLRELGGTKTEERSE"
     gene            5607..>5734
                     /locus_tag="VFR00_15350"
     CDS             5607..>5734
                     /locus_tag="VFR00_15350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013897031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lycopene cyclase family protein"
                     /protein_id="HEU0160698.1"
                     /translation="MSAETDFDYIVVGAGTAGCLLANRLCAHPATRVALLEAGGKD"
BASE COUNT         1030 a         1896 c         1887 g          921 t
ORIGIN      
        1 aaggcgccgt aataggtggt catggcggcc tgccgtggtc cgggatcgcc gtcgcttctg
       61 ccgccgtggg ccagagcctc ggcatgaaag ccgcgcacgg cctcctgcga gggggcgcgc
      121 aggcaaacgt ggctgcccgt atcggcggcg gcgggcgcca gatcggcgcg caggttcagc
      181 caaaactccg ggtagcgctt gccgaaaccg acgttggcgg ggcgctcgac gagcttggca
      241 taacccaagg gcgccaatac ccgggcatag aattgcgccg cgccggaaag gtcgcgcacg
      301 gcaatggaga cgtgatcgat catggcgccg ccccctatag cactttcggc tacttcgccg
      361 ataaggcggc cagcaccttc ggtggcgaca acggcagctc ggtcaggcgc aggccggtcg
      421 catgccgcac ggcgttggcc acggccgcca gtggcggcac gattggcacc tcgccgacgc
      481 cgcgcacccc ctgtgggtgc ctgtcgttgg gcacttcgat gagcacaggt tcgatcatcg
      541 gcaggtcgga ggcaaccggc atgcggtagt cgaggaagct cgggttatcg agcagaccgt
      601 tggccgcgaa gatgcactcc tcgttgatgg cccaaccgac gccttgcgcg accccgccct
      661 ggatctggcc ctccacgtag ctcgggtgga tggcgcggcc gacgtcttgg aaggccgtat
      721 agcgcacgat gcggagcttg ccggtctccg ggtcgatctc cacatcgcaa atgtgggtgg
      781 caaatccacc cgttgcgcct tccgtattga gcgatgagct gccattgatc ggaccgccgg
      841 tggcgctcgc cttggcggcg agctgggcca gcgtcaaagg cgcaaattcg ccggcgttgg
      901 cgccggcggg acgggccgcc ccgttctccc agatcacggc ttcgatatcg atcttccaaa
      961 gcttggcggc acgcgcgcgc aggctttcga tcacctcttc ggtcgcccgc gtcaccacca
     1021 tggcggaggc aaacgtcacg cggctgccgc cggtcaaagc actaaatccg atgacgctgg
     1081 tatcgccgat cagcgcctgt acctggcgat aatcgatgcc gagcatctcg gccacaatgt
     1141 tgacggtgga ggcgcgcgag ccgccgatgt cggggtgacc cgtcacgacg accacggtgc
     1201 catcctcgtt gacattgacc gtggcactcg actcgccgcc ggcgttgaac caaaagccac
     1261 tggcgacgcc gcgcccctgg ttgggaccga gcggggcctt gtagtgggga tggccgagcg
     1321 cggcctctaa cgtttcctgg tagccaatgc ggggaaagac gggaccgtag gccgccttgg
     1381 tgccttgctt ggccgcgttc ttctggcgca acagcaaggg gtcgacgccc aacgccgtgg
     1441 ccagctcatc gagcacacac tccaccgaaa aagccccgat tggcgccccc ggcgcccggt
     1501 aagcggcgac tttgggtcgg ttcgtcacca catcgaagcc gctgatctcg acattggcaa
     1561 tgtcgtaggg cgaaagcgcg cagccgaccg cgccgcggat gggagagccc ggaaaggcac
     1621 cggcctgcaa gcggtaggtc gccttgacgg cagtgatctt gccatccttg ctcgcgccga
     1681 tcttgaccgt gcttgaggag cccgaggtcg gaccggtggc gcggaacacc tcctcgcgcg
     1741 tcatcaccat cttgactggg cgcccggact tgcgcgccag caccaacgcc aatggctcca
     1801 gatagacgat ggtcttgcca ccgaagccgc cgccgatttc ggcgggcacg gctcgaatgt
     1861 cgctgagctt ggtgccggtc agctggcccg tcatgtcacg caccatgaag tggccctggc
     1921 tgctgctcca gatgttggcg cggccatcct tggtgacgct gacgagacat gcgtgcggct
     1981 cgatgtagcc ctgatgcacg ggcttggtct tgaagctccg ctcaatgatg acatcagcgg
     2041 cggcgaaacc cgcgtcgacg tcgcctagtt tgtgctcgag cttgccggca atattggagg
     2101 tcgtcgccac acccggtttg tcgccaaccc cgcgcatcgc ctcgtgcaga atcggcgccc
     2161 cgggctcgat cgcctcatcg acatcgatca cccaggggag gacctcgtag tcgacctcga
     2221 tcaatttgac ggccgcggcg gcaatgctgg ctgaggtggc cgcgacggct gccacggcgt
     2281 ggcccgcata aaatgcccga tcgcgggcca tgacgttgcg gctgatgaaa cgcaggtcct
     2341 ggatgccaag gatgacgggc ttgtcccagg gaaagtcgac cagatcgcgt cccgacatga
     2401 cggccttcac gcccgggagc gccagggcct tgtcgaactt gatcgccttg atgcgggcat
     2461 gcgggtgcgg gctgcgcagg accttgccgt agagcatgcc aggttgagag aagtcggcgc
     2521 cgaacgcggc ccgtcccgtc accttctcga cgccatcggg tcggatggtg cgcttgccaa
     2581 tccacttgtt attcgtcgaa ccgtttgccg tcatgcctgt tcttccatct cttgcgcgtc
     2641 ttgtgcagcg ctcctggagt ggggaggccg cgtccaccct cgaatagggc gcgcacggcc
     2701 tcaacacagc gggcgaaccg gcgcctatcc tataaggaag tgcgcctcga gcaactgccg
     2761 acggcgccgc acgggtatgc aggagccctc aagtgcctgg caggcaccgt ctaggctggc
     2821 cgtcaggggt gagagatgcg gggcgtctgc ggcgcgatct gcagcagcgc ctgggcccgt
     2881 gtgcagtcct ggcgcatctg gtccttgagg gcgtcgacgg cggcaaactt cgcatcaggg
     2941 cggacgaagg cgatgaactc cacctcgatc tcccggccat agaggttgcc ctcgaaatcg
     3001 aacaggaaca gctcgagcac cggctcgcca gcatcgaagg tcggccgagt gccgagatag
     3061 gcggcggcgt tgaagcggag gccctcgaag tatatgcgca ccgcgtagat gccgtggccg
     3121 agcgccacct cgcgcggcag tttcaggttg gccgtgggaa agccaagccc ggtgccccgc
     3181 ccggccccgc caacaacccg tccggacacg cgccagtaat ggcccatcat ctcggcggcg
     3241 cccccgacgt caccttgcgc gagctcggcg cgaatggcag aggaggaaaa cacctcgccg
     3301 gcttctccga tctgggagac gacgctgacg ccgaagccgt gcaccgcgcc tgcgcgccgc
     3361 agggcttccg gatcacccgt gcgcttggct ccgaaacgaa agtcatagcc gatcacgacg
     3421 tgatgcacgc cgaggccggc caccagcacg cgctccacaa aggcgtccgc gcctaaggcc
     3481 gcgaaggcgg catcgaaggg tagaacgaac gccacatcga gcccaaagcg ctcaagcagg
     3541 aggagcttgc gcggcagtgg cgtcagcgtg aagtgcggct tatccggctg aaagatggcg
     3601 cgcggatgcg gttcgaacag cagcgccgcc gccggacggc cgagctcgcg ggcaagaaga
     3661 cgcacctgcc cgagcagcgc ctgatggccg cgatgcacgc catcgaaatt gccgatcgtc
     3721 agcaccgagc cccggacggc ggcgggaaga tcgtcgtatc gctcaaagct ctgcatgggc
     3781 cttcagacca tcccaaggcc gagcgccaag cgcaaggcct tgcggcatcg accgcgccgc
     3841 gccattgctg cggccggcgg cgacccgccc cgcacggtag cccaatcgaa gaggggcctt
     3901 agcgcctggg cgcacgcgag gcgcgcttag ctcgcttttt gctcggccgc ccgggtcggc
     3961 accatcagca cggcctcgcc ttccagcaca accttgccgt cgacgctgca ggtgcactca
     4021 aagcgtgccc gccgcttggc gggatagagc tcaaccacgc ggaccttggc gatcacgcgg
     4081 tcgccgatgc gcaccggggc ccgaaagttc agcgtctggg agacatagat ggccccgggg
     4141 cccggcatct cgatcccgaa cacggccgag atatagcctg ccgtcagcat gccgtgggca
     4201 atcggctcct tgaaaatcgt cttggccgcg taagccgcat caaggtgaac cgggttgcgg
     4261 tcaccggtga cctccgcaaa gctgaggacg tccgcggccg agacggcctt ggcgaagctc
     4321 gcttccatcc cgagctcgag gtcttcaaag tggtagcctg gccgtgtctg gacgttcatt
     4381 gcatgccctt gaaatcgatt gccttctgcc gcggcgcaaa attagtcctg tacaagttgc
     4441 gccgcaacaa ggagctgacc gttatctgga caactcaaag ctcggggaaa agatcatgga
     4501 ctccctgagg atttgcggcc acaggcctcc cgggcgggct tttaagcggt tatctgctgc
     4561 gctgcaatca tgctgcgttg ccgcacatcc gcccgggttg cgtgccgcgc ccggcctggg
     4621 ctataagccg gctcctcagc gcattttttc gcctattggc ctgcacggga cccgccctgc
     4681 cggtcggaac gaaggtcctg ccatgagtga tcgcaaacct ttgatgccca aggccacggc
     4741 cgtttggttg gtggagaata cctcgctttc cttcgagcag atcgccgaat tctgcggcct
     4801 gcatgccttg gaggtgaaag gcatcgccga cggcgatgtt gcccacggca tcaagggcat
     4861 ggaccccatc tccaatggcc agttgagccg ggaggaaatc cgccgcggtg aggaggatca
     4921 cggccacgtc ctgcgcctgg cggtgtcgaa ggttgagatc ccggaagtca agaccaagaa
     4981 ggggccgcgc tacaccccgg tctcccgccg ccaggaccgg cccaacgccg tgatctggct
     5041 gttgcgcaat caccccgagc tgaaggacag ccagatcatg cggctggtgg gaaccaccaa
     5101 gccgacgatc gcggcgatcc gcgaccgcac gcactggaat tccgcgaacc tgacgccgca
     5161 ggacccggtc accctcggtc tgtgctccca gatcgacctc gacaatgagg tgaaaaaagc
     5221 cgcccggcgc gtggagcgcg agaaaaagga gcagcaaaag ccggccgaga agggcggcag
     5281 cctgctgccc acctccgaaa cgacggtggt gccggggctc ggcccgcagc cggacgtcga
     5341 gatcgtcccg cccgagcatg cggagccgac gcgcgaggaa accgaagcgg aggagcaggc
     5401 ccgcgtgctg gccaagctgc gcgaactggg gggcaccaag accgaggagc gctccgagta
     5461 agtccgccct cgctgggcga cgccagcggc cgctcgcgcg cactcgaggg gaccacggct
     5521 ggccggctgg acccaaacgc ctatccgggc catcttcaag cccgcgccca acgctcgatc
     5581 cttaggccta gcgccgagac gcgaagatga gtgccgaaac cgatttcgac tacatcgttg
     5641 tcggtgccgg cacggccggc tgcctgctgg ccaatcgtct gtgcgctcac cccgcaacgc
     5701 gcgtggcgtt gctggaggcc ggtggcaagg acaa
//
LOCUS       DASTMT010000662         4567 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_4915515, whole genome shotgun sequence.
ACCESSION   DASTMT010000662 DASTMT010000000
VERSION     DASTMT010000662.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4567)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4567
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4915515"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(114..953)
                     /locus_tag="VFR00_15355"
     CDS             complement(114..953)
                     /locus_tag="VFR00_15355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012110922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HEU0160699.1"
                     /translation="MAQATLEVRPIAGALGAEIHGVDLAGELAADTVAALRRAWLQHC
                     VIFFRAQPLSPAQFLTFAKRFGAIVEYPFIKGIEGFPEIIPVVKLEHEKTNFGGIWHS
                     DTSYLETPPMATMLIAREVPPMGGDTLFANTCLAYEALSPGMQRLLEGLIAVNSSRKA
                     DASRTREDRIKDMQRDDAKPEYVGEHPVVRTHPETGRKALYVNGAHSVRFRHMSEAES
                     APLLDYLIQHQTRPEFTCRFRWQVGSIAFWDNRCTQHNPVNDYHGYRRAMHRITLAGD
                     TPR"
     gene            1204..3135
                     /locus_tag="VFR00_15360"
     CDS             1204..3135
                     /locus_tag="VFR00_15360"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEU0160700.1"
                     /translation="MHGLSYRDAGAALGARLLPFALIFFLSQFGVRLSLALRVRGEAA
                     DGVWGFLAPFLIGAWFDLAVFCVCAVPVVLWWLLLPQSLKGGHLDRAATLAGAAFFVL
                     FCLILGIAEHLFWTEFGTRFNFIAVDYLIYTREVVQNIWQSYPIGKLFTFLLLLTSAG
                     TVLARRWLSPPGDGATLGVRWLPALAALMAPVVVVAATPTSLAYTRANAYANELSLNG
                     IWALTYAFFHNEIEYRRFYRTRPAEVVTKRLETLLAEKDAHLTSPGRDPLTRVVRRDG
                     RMLRKNVMLIGMESMGAEYMAHFGNRQNVTPHLDRLASEGLLFTQLLATGTRTVRGLE
                     ALSLSVPPTPGQSILRRPHNAGLFTLGDVFRERGYDTRFLYGGYGYFDNMNAFFAGNG
                     FEVIDRTSLSSEEIHFENVWGVSDEDLFQRVLKEADRSFAAGMPFFQLVMTTSNHRPF
                     TYPNGVIDIFSKTGRLGGVKYADHAIGGFVEAARGRPWFKDTLFVFVADHTAGTGGRI
                     ELDPTRYHIPLIFYAPDFVAPATYDRLASQIDVAPVLLGLLNTSYISRFYGRDVLNDR
                     DAVPRAFISTYEKVALVRGDTTLVLGPRQTIAAYAGLSKKPRADIDPELEADAIAYFQ
                     AASGWSQHSRRVGTLLHLD"
     gene            3196..3660
                     /locus_tag="VFR00_15365"
     CDS             3196..3660
                     /locus_tag="VFR00_15365"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEU0160701.1"
                     /translation="MTERGAAFKTSPSNPGLGEIQTRPERVADMPYAPAVRVEAPGDL
                     LFISGATPSPLYHKHPHELHEHNHPVGIAEQTRLAMDNIKTILDHQGLTWTDVVKVTK
                     YLTDMRDMDGMVAVLAEYFGSWTPASTSVCVNQLSTPGARVELDMIAVYPKG"
     gene            3692..4507
                     /locus_tag="VFR00_15370"
     CDS             3692..4507
                     /locus_tag="VFR00_15370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006609446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEU0160702.1"
                     /translation="MSLRSRCHDLCRRAAILAGLAAFALPAWAQSPPAAPTGAAGRAI
                     EDLVAANRILADQGVLDGFGHVSVRHPERPERLLLSRSLAPALTKPEDIMEYDFDCRP
                     VGDQSRTSFLERFIHCEIYRARPDVMAVIHTHSQGVIPFANSTVPLKAMFHVAGFLAA
                     GVPVFEIRQHAGMSNLLIGDAALGKALAGTLGDKSVVLMRGHGAVVVGPSIQIAVYRA
                     IYTDVNARLQAQAIALGGPVTYLDKEEGEKTDATQQQVVLRPWELWKQKAMKP"
BASE COUNT          733 a         1581 c         1422 g          831 t
ORIGIN      
        1 ttgaccaccg gcttgacctc ccgttcgagc cagcgctcga tgcttctgag cagctgctct
       61 tcgtccgcgc gttcgatctg gcgcagcgcc gcttcggtca tgcctcatcc tctctagcgc
      121 ggggtgtcgc cggcaagtgt aatgcgatgc atggcgcgcc gatagccatg gtagtcgttg
      181 acgggattgt gctgggtgca gcggttgtcc cagaaagcga tcgagccgac ctgccagcga
      241 aagcgacagg tgaactccgg tcgcgtctgg tgctggatga gatagtcgag gagcggcgcg
      301 ctttccgctt cgctcatgtg cctgaagcgt acgctgtgcg ccccgttgac gtagagcgct
      361 ttgcgcccgg tttcgggatg cgtgcgcacc acgggatgct cgccgacata ttccggcttg
      421 gcatcatcac gctgcatgtc cttgatgcga tcctcgcgcg tgcggcttgc atcggccttg
      481 cgcgagctgt tgaccgcaat caggccctcg agcaggcgtt gcatgcccgg cgacagcgcc
      541 tcgtaagcca agcaagtgtt ggcgaacaag gtgtcgcccc ccatgggcgg cacctcgcgg
      601 gcgatcagca tggtggccat gggcggcgtc tccaaatagg aggtgtcgga gtgccagatg
      661 ccaccgaaat tggtcttctc gtgctcgagc tttacgaccg gaatgatctc agggaagccc
      721 tcgatcccct tgatgaaggg gtattcgacg atggcgccga agcgcttggc aaaggtcaaa
      781 aactgcgccg gcgacagcgg ctgcgcgcgg aagaagatga cgcagtgttg caaccaggca
      841 cgccgcaggg ccgccacggt gtcggccgcg agctcgccgg cgagatcgac gccgtgaatt
      901 tcggcgccga gcgccccggc gatcggccgc acctcgagtg tcgcctgggc catggcgtct
      961 ttgcccctcc tcgcgggcgc ctgcgggcaa aaaccctaac acgccgccgc gcctggaaaa
     1021 gggggtgcgc cttgcccggc cgaggccccg ttttgccccc ggcggccgcg cccggccgcc
     1081 tggcggcgcg ctttgcgcgc ctgcggcatc ttgagccccc accacccccg ccccggcatc
     1141 ggaaaaaagc cgcaaagcta tccctagtgc ctaaaggcaa ggggaattgt ggggacaggc
     1201 acaatgcacg ggctcagcta tcgcgacgcc ggtgcggcgt tgggcgccag gctcctgcca
     1261 tttgccctca tctttttcct cagccagttc ggcgtgcgcc tgagcctggc gctcagggtc
     1321 aggggcgaag ccgccgacgg tgtctggggc ttcctcgccc cgttcctgat cggggcctgg
     1381 ttcgatctgg cggtcttttg cgtgtgcgcg gtgcccgtgg tgctgtggtg gctgctgctg
     1441 ccgcaatccc tcaaaggcgg ccacctcgac cgcgccgcca ccttggcggg ggctgcattc
     1501 ttcgtgttgt tttgcctgat cctcggcatt gccgagcacc tgttctggac cgagtttggc
     1561 acgcgcttca atttcatcgc cgtcgactac ctgatctata cgcgcgaggt ggtgcagaac
     1621 atttggcagt cctacccgat cggcaaactg ttcaccttcc tgctgcttct cacctcggcc
     1681 ggcacggttc tggcgcgccg ctggctctcg ccgcccggcg acggcgcgac cctcggcgtg
     1741 cgctggctgc cggcgctcgc cgccctcatg gctcccgtcg tggtggtggc tgccaccccg
     1801 acctcgctgg cctacacgcg cgccaatgcc tacgccaacg agctctcgct caacggcatc
     1861 tgggccctga cctacgcctt cttccacaac gagatcgagt accgtcgttt ctatcggaca
     1921 cgacccgcag aggtggtgac aaaacggctc gagacgttgc tcgccgaaaa ggatgcccac
     1981 ctcacctcac cgggacggga ccccttgacc cgcgtcgtgc gccgcgacgg ccgcatgctg
     2041 cggaagaacg tcatgctgat cggcatggaa agcatgggcg ccgaatacat ggcccacttc
     2101 ggcaatcggc agaacgtcac gccgcatctc gaccggctcg ccagcgaagg cctcctgttc
     2161 acccagttgc tggccaccgg cacgcgcacc gtgcgcggcc tggaggcgct gagcctatcg
     2221 gtgccgccga cgcccggaca atcgatcctg cgccgccccc ataacgcggg cctattcacg
     2281 ctcggcgacg tgttccgcga gcgcggctac gacacgcgct ttttgtacgg gggctatggc
     2341 tatttcgaca atatgaacgc gtttttcgcc ggcaacggct tcgaggtgat cgaccgcacg
     2401 tcgctcagca gcgaggaaat ccatttcgaa aatgtctggg gcgtgtcgga tgaggacctg
     2461 ttccagcgcg tgctcaagga ggccgatcgc tcctttgctg cggggatgcc ctttttccaa
     2521 ttggtgatga caacctccaa ccatcgaccc ttcacctacc ccaatggggt gatcgacatt
     2581 ttttccaaaa ccggccggct cggcggcgtg aagtacgccg atcacgcgat cggcggcttt
     2641 gtggaggcgg cccgcggcag gccctggttc aaggacacct tgtttgtgtt cgtcgccgac
     2701 cataccgccg gcaccggtgg ccgcatcgag ctcgatccga cgcgctacca cattcccttg
     2761 atcttctacg cccccgactt tgtggcgccc gccacctacg accgtctcgc cagccagatc
     2821 gacgtggccc cggtcctgct cgggctgctc aataccagct acatcagccg cttttacggg
     2881 cgcgatgtgc tcaacgaccg ggacgccgtg ccgcgcgcct tcattagcac ctacgagaaa
     2941 gtcgcactgg tgcgcggcga caccaccctg gtgctcgggc cgcggcaaac cattgccgcc
     3001 tacgccggtc tcagcaaaaa gccacgcgcc gacatcgatc ccgagctcga ggccgacgcc
     3061 atcgcctatt tccaggcggc ctcgggctgg tcgcagcact cgcgccgcgt cggcaccctg
     3121 ctgcacctgg attgatgctg ggcggcggct cgggcaggat gctcgcgcaa agccccatcc
     3181 ccagcggagc ctggcatgac cgagcgcggc gcggccttca aaacttcgcc tagcaatccc
     3241 ggcctcggcg agatccagac gcgccccgag cgcgtcgccg atatgcccta tgcgccggcg
     3301 gtccgtgtcg aggcgccggg cgacctcttg ttcatctcgg gtgccacccc ctcgccgctc
     3361 tatcacaagc acccgcacga gttgcacgag cacaaccacc ccgtcgggat cgccgagcag
     3421 acgcgcctgg ccatggacaa catcaagacc atcctcgatc accagggctt gacctggacc
     3481 gatgtcgtca aggtcaccaa gtacctgacc gacatgcgtg acatggacgg catggtggcc
     3541 gtgctggcgg agtacttcgg cagctggacg ccggcgagca cgtcggtgtg cgtcaaccag
     3601 ctgtcgacgc cgggggcgcg tgtcgagctc gatatgatcg ctgtctaccc gaaaggctaa
     3661 ccccaaacgc ggcttcgccg ccgaggtgag catgtccctt cgatcccgct gccacgacct
     3721 ttgccgccgc gccgccatcc tggcggggct tgccgctttc gctttgcccg cctgggcgca
     3781 atcgcccccc gcggcgccga ccggggcggc cgggcgcgcc attgaggact tggtcgcggc
     3841 caaccgcatt ctcgccgatc agggcgtgct cgacggcttc ggccatgtca gcgtgcgcca
     3901 tcccgaacgc cccgagcggc tcctcctctc gcgctcgctg gccccggcgc tgaccaagcc
     3961 cgaagacatt atggagtacg acttcgactg ccggcccgtc ggcgatcaga gcaggaccag
     4021 ttttctcgag cgcttcattc attgcgaaat ctaccgggcg cggcccgacg tgatggccgt
     4081 gatccatacc cattcgcagg gggtcattcc cttcgccaac agtaccgtgc cgctgaaggc
     4141 catgttccac gtggcaggct tcctcgccgc gggcgtgccc gtattcgaaa tccgccagca
     4201 tgccggcatg agcaatctgc tgatcggcga tgccgctctg ggcaaggcct tggcaggcac
     4261 gctgggcgac aagtccgtcg tcctgatgcg tggccacggc gccgtcgtgg tgggacccag
     4321 catccagatc gcggtctatc gcgccatcta caccgacgtg aacgcgcgcc tgcaggccca
     4381 ggcgatcgcg ctcggtggcc ccgtcaccta tctcgacaag gaagaggggg agaagaccga
     4441 tgccacccag cagcaggtcg tactgcggcc ctgggaactc tggaagcaga aggcgatgaa
     4501 gccctagccc caggacttca ccgcctgtat cggctgcatt gacactgggc ggtcgcggcc
     4561 gcccgcg
//
LOCUS       DASTMT010000663         1721 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_4918354, whole genome shotgun sequence.
ACCESSION   DASTMT010000663 DASTMT010000000
VERSION     DASTMT010000663.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 1721)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1721)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1721
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4918354"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..977
                     /locus_tag="VFR00_15375"
     CDS             <1..977
                     /locus_tag="VFR00_15375"
                     /inference="COORDINATES: protein motif:HMM:NF013143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEU0160703.1"
                     /translation="IGIVYAVSTALGVIALDRAPLGAEHIKQLLIGSIITVSPQDVGV
                     LALIYGSIGVVHVLCRRPLLQASLQGQAAAGRGAFGWDLLFYVSFALVVTSSVRVAGV
                     LLVFSYLIVPAVLAGLLASAFARRLLIAWALGSLLTGLGLYASWTWDLPTGPAIVSTF
                     GAAVGVVVLGRAATRLTRRRLAVAASGMAIFAAVPLLSFPHLEQPWLDALEDVAPPLE
                     TAFLDAGERQLRAEAIESIKQARAELTRLRALEQDVRWGKEAMDADAAERLRQYLAGK
                     SEISAGDELSLRHLRGKARERQRLTLGLPLLLAGAYGLYRLSLRTTSP"
     gene            1259..>1721
                     /locus_tag="VFR00_15380"
     CDS             1259..>1721
                     /locus_tag="VFR00_15380"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEU0160704.1"
                     /translation="MSRLQADVLLLFTALIWGVAFLFQKSATAHVGPLTFLALRGLIA
                     CLALAPLALAEARRAASPARADFYLTSLAGGVAFLIAGWLQQAGLETASVTNTGFLTA
                     LYVVLTPLIVLGWSGKMPNPLVWPAVGMSALGTWLLGGGGIGAFSRGDSMVA"
BASE COUNT          210 a          575 c          593 g          343 t
ORIGIN      
        1 tgatcggcat cgtttatgcc gtgtcgaccg cgctgggcgt gatcgcgctc gaccgcgccc
       61 cgctgggcgc cgagcacatc aagcagctgc tgatcggctc gatcatcacc gtcagccccc
      121 aggacgtggg tgtgctggcg ctcatctatg gcagcatcgg tgtcgtgcac gtgctgtgcc
      181 ggcggccgct gctgcaggcc tcattgcagg ggcaggccgc cgcaggacgc ggtgccttcg
      241 gctgggacct cttgttctac gtctcctttg ccctcgttgt gacgtcctcg gtgcgcgttg
      301 ccggcgtgct cctggtcttt agctatttga tcgtgccggc cgtcctcgcc gggctgctgg
      361 cgtcggcttt tgcgcggcgc ctgctcatcg cctgggcgct gggttcgctg ctgaccggcc
      421 ttggcctcta cgcctcctgg acctgggatc tgccgaccgg gccggccatc gtgtcgacgt
      481 ttggcgccgc ggtcggggtt gtcgtgctcg ggcgtgccgc cacacgcctg acccggcgcc
      541 ggctcgccgt cgccgccagt ggcatggcca tctttgccgc cgtgccgctg ctgagctttc
      601 cgcacctgga gcagccctgg ctcgatgccc tggaggacgt cgcgccgccc cttgaaaccg
      661 ccttcctcga cgccggtgag cgccagttgc gggcggaggc gatcgaatcg attaaacagg
      721 cgcgggccga actgacacgg ctcagagcgc tcgaacagga cgtgcgctgg ggcaaggagg
      781 ccatggatgc ggatgctgcg gagcggctca ggcaatatct ggccggcaaa agcgagattt
      841 ccgcgggcga tgagctctcc ttgcgccatc ttcgcggcaa agcgcgcgag cgacaacggc
      901 taactctcgg gctaccgttg ctgctggccg gcgcctatgg cctctatcgt ctcagcctgc
      961 gcaccacctc gccctgaggc tgtggcgata ggtccagtcg gagggtcgcc aaaagcgcca
     1021 acccgcgcgc ggtcctgtca cacaggttgg ctaaggcagg tttgccgccg caacggagcc
     1081 cagggggatc ctcgccgagc ccgcgcgagc tgggcgcgtg tcgtctcgcg ggccgcaagt
     1141 ccgcgcgttt agctgatctt ttcaaaccta ccttgggcat ttaagccgcg gtgatccggc
     1201 gcccgggtct cggcttggcc caggttggcg agcgcagtcg gaggaaggct tgacgcccat
     1261 gtcacgtttg caagccgacg tcctcctgtt gtttacggcc ctgatttggg gcgtggcgtt
     1321 cctgttccag aagtcggcca cggcgcacgt cggaccgctg acgtttctgg cgctgcgcgg
     1381 cctcatcgcc tgtctggccc tagcgccgct ggcactcgcc gaggcacggc gcgcggcctc
     1441 gccggcccgc gccgactttt atctgacctc gctcgccggc ggcgtggcgt tcctgatcgc
     1501 gggctggctg cagcaagccg gcctggagac cgccagtgtc accaataccg gcttcttgac
     1561 ggcgctttac gtcgtcctga cgccgctgat cgtgctgggc tggagcggca agatgcccaa
     1621 tcctttggtg tggccggcgg tcgggatgtc tgcgctcggc acctggctgc tgggtggtgg
     1681 tgggatcggc gccttttcgc ggggcgatag catggtcgcg a
//
LOCUS       DASTMT010000664         4450 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_3918717, whole genome shotgun sequence.
ACCESSION   DASTMT010000664 DASTMT010000000
VERSION     DASTMT010000664.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4450)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4450)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4450
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3918717"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..841)
                     /gene="nudC"
                     /locus_tag="VFR00_15385"
     CDS             complement(<1..841)
                     /gene="nudC"
                     /locus_tag="VFR00_15385"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF001299.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HEU0160705.1"
                     /translation="MPTVGSTTLLDRLGPKRNDAEFIKAKLAAPGARFLVLADLKPVI
                     RSNPERTAAKLAWFSGEELAQFGLPIADALFLGADPAGHGHFAISVTEHRTRNVPGAV
                     EKLRPIVDLRSLAMQGAMSPEELSLCGQARALAQWHENARCCGHCGGTTLVKDGGWRR
                     KCWACGLDWFPRTDPVVIMLISDGPRCLLAHEHRYGDKMFSALAGFIEPGEDIEHAVR
                     REVMEETGIRVGEVRFHSSQPWPFPHSLMLGCMGRAQTAEITVDTTEIAEARWFPRQE
                     ARQM"
     gene            1124..1220
                     /gene="ffs"
                     /locus_tag="VFR00_15390"
     ncRNA           1124..1220
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VFR00_15390"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            1276..3042
                     /locus_tag="VFR00_15395"
     CDS             1276..3042
                     /locus_tag="VFR00_15395"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEU0160706.1"
                     /translation="MAKKKSTSASASAPAAGTDAPGSAAPAPYLVLARKYRPTTFHDL
                     VGQEAMVTTLRNAFATDRIAQGYMLTGVRGVGKTTTARILSRALNYERDGVSKPTFDM
                     PEFGRHCAEIMEGRHPDVLEMDAASNTGVDNVREIIESARYKPLLARYKVYLIDEVHM
                     LSKGAFNALLKTLEEPPGHVKFIFATTEVRKVPVTVLSRTQRFDLRRVDVPLLVEHFR
                     KIVANEGAEAEDAALNLIARASEGSVRDGLSLLDQALAMGSGSVVSERVRAMLGLADR
                     GRIFDLLELLLSNAAREAVLAFGALNRDGAEPGQVLADLAEAVHIATRAKTLGAEAAG
                     DGLSAEERRRASALGERLSVPILARAWQMLLKGLEEVAAAPNQAAAAEMVLIRLAFTA
                     DLPAPDEILQALGGVPVARRGAPAPAAPARQPALESSLDAAWPEGEDEEADGENSARE
                     APRPPLLLSFADVVRLAGKRREAKLKVHLEEHVSLVKFDPAGSIELNLLPGAPKELAN
                     ELREKLNLWTQGRWMVAVSTAPGEPPLGEAERARAAAEIAEIRKHPAVAAVLQQFPDA
                     EISSVRPLSGLEADDADHTGTG"
     gene            3120..3440
                     /locus_tag="VFR00_15400"
     CDS             3120..3440
                     /locus_tag="VFR00_15400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEU0160707.1"
                     /translation="MKDLMNMMKQAQEIQGRMQQLQDELANLEVEGQSGAGLVKVKLN
                     GKGDARAIKIDQSLIKPEESEILEDLIVAALQDAKSKVETAVQAKMQEITGGLALPPG
                     LKLF"
     gene            3495..4097
                     /gene="recR"
                     /locus_tag="VFR00_15405"
     CDS             3495..4097
                     /gene="recR"
                     /locus_tag="VFR00_15405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011179035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HEU0160708.1"
                     /translation="MNRKSSGSEIERLVGLLARLPGLGSRSARKAALALLKRRTELLE
                     PLAAALSAAAAKIVECSTCGNLDTISPCTICQDPRRDATLIVVVEEIGDLWALERAGV
                     VNGLYHVLGGHLSPLDGVGPERLNIGRLIERAVPPVQEILLALNATVEGQSTAHYLTT
                     QLQGRGIAVTRLAQGVPIGGELDYLDDGTLAAAIKSRRTL"
     gene            4208..>4450
                     /locus_tag="VFR00_15410"
     CDS             4208..>4450
                     /locus_tag="VFR00_15410"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEU0160709.1"
                     /translation="MGVHLGYLLPTREHVMAGRSEAGSLLGLASRAADLGYDSIWVGD
                     SVLARPRHDPLTLLAALAARVPRLELGTAVLLPALRN"
BASE COUNT          775 a         1445 c         1489 g          741 t
ORIGIN      
        1 gcatttgccg tgcctcttga cgcggaaacc agcgcgcttc ggcgatctcg gtggtgtcga
       61 ccgtaatctc ggccgtttgc gcccggccca tgcatcccaa catcaaggag tgtgggaacg
      121 gccaaggctg gctggaatga aaacgcacct cccccacgcg gatccccgtc tcttccatga
      181 cctcgcgccg caccgcgtgc tcgatgtcct cgccaggctc gatgaagccc gccaaggcgg
      241 aaaacatctt gtcgccatag cgatgctcgt gcgccaacag acagcgcgga ccgtcgctaa
      301 tgagcatgat caccaccggg tccgtgcgcg gaaaccagtc gaggccgcag gcccagcatt
      361 tgcgccgcca gccgccatcc ttgacgaggg tggtgccgcc gcagtgacca caacagcgcg
      421 cgttttcatg ccactgggcc agcgcgcggg cctggccaca cagcgacagt tcctccggcg
      481 acatcgcgcc ttgcatggcg agcgagcgca aatcgacgat tggccgcagc ttctctacgg
      541 caccgggcac gtttcgggtg cgatgctcag tgacggaaat ggcgaaatgg ccgtgtcccg
      601 ccggatcggc gcccaggaac agggcatcgg cgatcggcaa gccgaattgg gcaagttctt
      661 ccccggaaaa ccaggcgagt ttcgccgctg tgcgctcggg gttggatcgg atcaccggct
      721 tcaagtccgc cagcacgaga aatcgcgcgc ccggcgccgc cagctttgcc ttgatgaact
      781 ccgcatcgtt cctcttcggt ccgagccgat cgagaagtgt ggtcgaaccg acggtcggca
      841 ttactgcgct ccctgactgg ctgccatctt tgccaattat gtaaacgctt cgggctgggc
      901 aaagccggcc gcggcacggc caagctcggc gcacgcccac gcgcgccaca ggggtgcatg
      961 atatggccaa cgtttggcaa gcgcacgccg aaacgattgc gcgctgcggc gttgcgatta
     1021 agccgttgcg ggcgcccgtt cgcgcggcac cgcgaccctg gcgccggtgg gcgaacgatc
     1081 gggcgggttg taattggctt gcgcgcccac cataatcgga ccgggaggtt ggcggcggac
     1141 gtgtccctcg ccaaccgggt caggtccgga aggaagcagc cctaacgagt aagatacggg
     1201 tcgtttgttc agcctcccgc ctcggccgcc cgctcctttg cgattggggc gctgttgaag
     1261 gtgcgaacgg ccaacatggc aaagaagaaa tccacgtccg cttccgcttc cgctccggcc
     1321 gcaggcacgg acgcgccagg atcggccgcg cccgcaccct acctcgtgct ggcccgcaaa
     1381 taccggccga cgacgttcca cgacctggtt ggccaggagg cgatggtaac gacgctcagg
     1441 aatgcgtttg ccaccgatcg gatcgcgcaa ggctatatgc tgacgggcgt gcgcggcgtg
     1501 ggcaagacca ctaccgcgcg catcctctcg cgtgccctca actatgagcg cgacggcgtc
     1561 agcaaaccaa ccttcgacat gccagagttc ggccgccact gcgccgaaat catggaaggc
     1621 cgtcaccccg acgtcttgga gatggatgcc gcctccaaca ccggtgtcga caatgtccgc
     1681 gagattatcg aaagcgcccg ctacaagccg ctcctcgccc gctacaaagt ctacctgatc
     1741 gacgaagtgc acatgctgtc aaaaggtgcc ttcaatgcgc tgctcaagac gctcgaagaa
     1801 ccaccgggac acgtcaagtt cattttcgcc accaccgagg tgcgcaaggt tccggtcacg
     1861 gtcttgtccc gcacccagcg cttcgatctg cgccgcgtcg atgtgcccct gctcgtcgag
     1921 cattttagaa agatcgtggc caacgagggc gccgaggccg aggatgcggc gctgaatttg
     1981 atcgcacggg cctccgaagg ctccgtgcgc gatggtcttt ctctgctcga tcaggcgctt
     2041 gccatgggtt cgggcagcgt cgtgagcgag cgcgtgcgcg ccatgcttgg cctggccgat
     2101 cgcggtcgca tttttgacct gctcgagctg cttttgagca acgcggcgcg cgaagccgtc
     2161 ttggccttcg gcgctctcaa tcgggatggt gccgagcccg gccaggtgct cgccgatctc
     2221 gccgaagccg tgcacattgc cacgcgggcg aagacgctgg gcgccgaggc ggccggcgat
     2281 gggctctcag cggaggaacg gcgccgcgcc agcgcgctcg gggagcggct ctcggtgccg
     2341 atcctggccc gggcttggca aatgctgctg aaggggctcg aggaggtggc agcggcgcca
     2401 aaccaggccg cggcggccga aatggtgctg attcgcctgg cgtttacggc cgatctgccc
     2461 gcccccgacg agatattgca agccctgggc ggggtgcccg ttgcccgacg cggcgcgcct
     2521 gcgccggcgg cccctgcacg ccagccggct ctagaaagct ccctcgatgc ggcctggccc
     2581 gagggggaag acgaggaggc ggacggggag aattccgccc gcgaggcgcc gcggccgccg
     2641 ctgctgttgt cctttgccga cgtggtccgc ctggcgggca agcgccgcga ggccaagctc
     2701 aaggtccacc tagaagagca cgtgagcctt gtgaagttcg atccggcggg tagcatcgag
     2761 ctcaatcttt tgccgggcgc gccgaaagag ctcgccaatg agctacgcga aaaacttaat
     2821 ctatggacgc aggggcgctg gatggtggcc gtgagcacgg cccctggcga gccgccgctg
     2881 ggcgaggcgg agcgcgcgcg cgcggctgcc gagatcgccg aaattcgcaa gcatccggcc
     2941 gtcgccgccg tgctgcagca gtttccggac gccgaaattt ccagcgtgcg tcccctctcg
     3001 ggacttgagg ccgatgacgc cgaccatacc ggaaccggct aattggttgg gtggtgcagg
     3061 atgaaccaag gcggttcgcg tggggagctt tgccgccaag ccgcgtgcac ggagatgcta
     3121 tgaaagatct gatgaatatg atgaagcagg cccaggaaat ccagggccgc atgcagcagc
     3181 tgcaggacga gctcgccaat ctcgaggtgg agggccaatc cggcgccggg ctcgtgaagg
     3241 tcaagctcaa cggcaaggga gacgcgcgcg cgatcaaaat cgatcagagc ttgatcaagc
     3301 ccgaagagag cgaaatcctc gaggatctca tcgtggccgc cctgcaggac gccaaaagca
     3361 aggtcgaaac ggcggtgcag gccaaaatgc aagagatcac cggcgggctc gcattgccgc
     3421 ccgggctcaa gcttttttga ggatcgcaag gtgagcttcg gcaggttgcg ggaagatcgg
     3481 cgcgtcggcg aacgatgaac cgaaaatcct caggcagcga gatcgaacgc ttggttggcc
     3541 tgctggcgcg attgccggga ctcggctcgc gctcggcgcg caaggccgct ttggcgctat
     3601 tgaagcgtcg caccgagttg ctggagccgc tggccgccgc gctcagcgct gctgcggcca
     3661 agattgtcga atgcagcacg tgcggcaatc tcgataccat ttcgccatgc acgatttgcc
     3721 aagacccgcg gcgcgatgcc accctgatcg tggtggtgga ggagatcggc gacctgtggg
     3781 cgctcgagcg tgccggcgtc gtcaatgggc tctaccatgt tttgggtggg catctttcac
     3841 cgctcgacgg ggtcggcccc gagcggctca acatcggccg cttgatcgag cgggccgtgc
     3901 cgccggtgca ggagatcctg cttgccttga acgccacggt cgaaggccaa tccaccgccc
     3961 actatctcac cacgcaattg cagggccgcg gcatcgccgt gacgcggctg gcccagggcg
     4021 tgccgatcgg cggtgagctc gactatctcg acgatggcac cctggcggcg gcgatcaaaa
     4081 gccggcgcac gctctaagcc tgggcatgcc tgggcgctgg cgccctcgca aacgccgctt
     4141 gccgcgtcga gcccggcggg gcaggctcgc agcggttgcg ggcgtctgct cgcgagggag
     4201 gatccccatg ggcgtgcatt tgggatacct gctgccgacc cgcgaacacg tgatggcggg
     4261 gcggtcggag gcgggctcgc tgctcgggct cgccagccgg gcagcagacc tgggctatga
     4321 ctcgatctgg gtcggcgact ccgtccttgc ccgtccgcgc cacgatccgt tgaccttgct
     4381 tgccgcgctg gccgcgcgcg tgccgagact tgagctcggc acggcggtgc tgctgccggc
     4441 gctgcgcaac
//
LOCUS       DASTMT010000665         6283 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_6474390, whole genome shotgun sequence.
ACCESSION   DASTMT010000665 DASTMT010000000
VERSION     DASTMT010000665.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 6283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6283)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6283
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6474390"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..516
                     /locus_tag="VFR00_15415"
     CDS             <1..516
                     /locus_tag="VFR00_15415"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009491553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD-dependent protein deacetylase"
                     /protein_id="HEU0160710.1"
                     /translation="PNAGHRALARLVEQGKMLAIITQNIDGLHQASGVADDKVIELHG
                     NSTYAACLDCRRRYELDWVREIFAVDEQLPLCTRCGGMIKTATISFGQAMPEAEMERA
                     HAVTLAADVFIVLGSSLVVYPAAGFPILAKRNGARLVIVNRDPTEQDELADLVINAEI
                     GATMSRAVGVN"
     gene            621..1358
                     /locus_tag="VFR00_15420"
     CDS             621..1358
                     /locus_tag="VFR00_15420"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011443039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEU0160711.1"
                     /translation="MLEQTLDIPGKAGAMETFMARPERGLYPAVLFLMDAPGIREELR
                     DMARRLASVGYCVLLPNLYYRAGRDSIYGPDVLVHGSAAHQRMRAVRTTMTIPPVMDD
                     IAAILAFLDSADGVKRGAVGVHGYCMSGPYALAAAARYPERVAAAASFYGTRLISDAV
                     ESPHLALARAKAELYIACAEIDDLAPLPMVAELEQLFAAAGNPGELEIYPGVHHGFAF
                     PQRKIYDQAAAERHWERLIALYRRRLG"
     gene            complement(1399..2274)
                     /locus_tag="VFR00_15425"
     CDS             complement(1399..2274)
                     /locus_tag="VFR00_15425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018322917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU0160712.1"
                     /translation="MRTLNIGEVTITSIIERDGPWRKPEDMFPAYDPEIGRRHLVEMD
                     PVVFDPASGKMVITYQTFVVRTPKHTVLIDTCTGEDKGHPPPMDFPKQPWLDNFRAAG
                     LAFDDITHVFCTHLHIDHTGWNTMLRDGRWVPTFPNAKYIFHKDEYAYWEAATQRGAN
                     PPGNVWTYNCRPIVEAGQALLVADTYQLDDTFSLIPTRGHSPHHCCVEIRSQGQVAVV
                     TGDMMHHALQCREPQWSTIFDTDKEQAHQARRQFLGKVADTSTLLLPIHFPNPTVGRV
                     SADGERFRYNFVRAT"
     gene            2475..3245
                     /locus_tag="VFR00_15430"
     CDS             2475..3245
                     /locus_tag="VFR00_15430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015684187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU0160713.1"
                     /translation="MSGLLEKHIAAVTGAGSGIGRAIALGYGKAGAGVVVLDANAETA
                     QETATAIKSEGGKAWSFQLDVRDGEACRTLAAVVAISVGQISILVNNAGINRRNAFTA
                     EPAAVLKDWQDIMAVNLDGIFNVTHAFLKDLRQSRGRIINIGSIQSFVHVRTPNSPAY
                     TTSKHGVLGFTRALAAELGKDGVRVNAIGPGLIETPLNAQVRANNPELVRTFLEHTPL
                     ARAGKPEDIVGPAVFLASDLSAYVTGAIIMVDGGYRAI"
     gene            3509..4174
                     /locus_tag="VFR00_15435"
     CDS             3509..4174
                     /locus_tag="VFR00_15435"
                     /EC_number="2.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0018104 - peptidoglycan-protein
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEU0160714.1"
                     /translation="MNVRPLLHLLISSLVAVLAALVAPPALAQQLFWDWGGGDTVGAS
                     GREIVRFSERFAPGQVVVSFGDRRLYFVTRPGEALSYPIAIPRDEDRWEGVTNVTNKR
                     VNPSWTPTPTMMAENPRLPRWVPGGHPMNPLGVRALYLGASAYRIHGTDAPWTIGTAV
                     SKGCIRMYNQDVLDLYPRVELGATVTVTWDRFQSGGNYASTPRAAAPRLREMRQSDLP
                     RSF"
     gene            complement(4177..4485)
                     /locus_tag="VFR00_15440"
     CDS             complement(4177..4485)
                     /locus_tag="VFR00_15440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0160715.1"
                     /translation="MAAAPNLLVEETARAIERLQTRLARHRAQLADLSPDSWEVSKAT
                     GKIAQMEKGLARLQLYSEMLSSEASRTDKRLIRERDQFAKQWKRKWRPSRAPQPEEDR
                     "
     gene            complement(4819..5706)
                     /gene="panB"
                     /locus_tag="VFR00_15445"
     CDS             complement(4819..5706)
                     /gene="panB"
                     /locus_tag="VFR00_15445"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HEU0160716.1"
                     /translation="MTGDAKSAQKKREKRSITEIRQSKKTGEKMVYMSVPDYTSAKWA
                     EMAGVDVAVVGDSLAMIAHGHPSTVPATMDMMVLHAQAIRRGAPNTFVLGCMPYQSYH
                     TIERALTNATRFMQEAGSDAVKPQGGKSQAHILRALVDAGIPTASHIGLTPHTIAMFG
                     GFKIQGRTAEAAMKILEDALAIEDAGCFMLEFEAVPAKIAKLISEQLTIPTIGIGAGP
                     GTDGQILLCHDLLGVFTDFKPKFTKRFAKLTEVAVAGIQQYVKEVKEGAFPDDDHSYT
                     VNEAEYEKFARLVEKRRQL"
     gene            complement(5719..6090)
                     /locus_tag="VFR00_15450"
     CDS             complement(5719..6090)
                     /locus_tag="VFR00_15450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0160717.1"
                     /translation="MALPPLPELAVFMALVLVLALYGLTVSGHFPAEYRAPALSTTAG
                     QVLLWGSLMLCTALTVLLAGFAWRHVPLYAAAIGGGAMLLAAPLILRPLPDSFVNGRG
                     ALLFFAGLALCLALIAQTLIA"
BASE COUNT         1054 a         2032 c         2088 g         1109 t
ORIGIN      
        1 ccgaacgccg ggcaccgcgc gctcgctcgt ctggtcgagc aaggcaagat gctggcgatc
       61 atcactcaga atatcgatgg cttgcatcag gcctcggggg tggccgatga caaggtcatc
      121 gagttgcacg gcaactccac ctatgcagcc tgcctcgatt gccggcggcg ctacgagctc
      181 gactgggtgc gggagatctt cgccgtcgac gaacaattgc cgctctgcac gcgctgcggt
      241 ggcatgatca aaaccgcaac catctcgttc gggcaggcca tgcccgaggc cgagatggag
      301 cgggcgcacg cggtcacgct ggccgcggac gtcttcatcg tgctcggcag ttccctcgtc
      361 gtctatcccg cggcgggctt tcccatcttg gccaagcgca atggtgcgcg tctcgtcatc
      421 gtcaaccgtg acccgaccga gcaggacgag ttggccgatc tcgtcatcaa tgccgagatc
      481 ggcgcgacca tgagccgggc ggtcggcgtc aactgacagg cgcgcgcgcg ccccgcaggt
      541 tcggatctgg gcccgagaac ccgccggctc gcccataggg gctcgcgccc gcgccagtcg
      601 gcgtctcgag gaggcaggcc atgctcgagc agaccttgga tatcccgggc aaagctggcg
      661 ccatggagac gttcatggcg cggccggaac gcggcttata cccggccgtc ctgttcctga
      721 tggatgcccc gggaattcgc gaggagttgc gcgacatggc ccgccgcctc gcgagcgtcg
      781 gctattgcgt gctgctgccc aatctctatt accgggccgg gcgcgacagc atctatggcc
      841 ccgacgtgct ggtgcacggc agcgccgcgc atcagcggat gcgcgccgtg cgcaccacga
      901 tgacgattcc gccggtaatg gacgatatcg ccgccatcct ggccttcctc gacagcgcgg
      961 acggcgtgaa acggggcgcc gtcggggtgc atggctattg catgagcggg ccctacgccc
     1021 ttgccgcggc cgcgcgctat cccgagcgcg tcgccgccgc ggcgtcgttc tacggcacca
     1081 ggctgatcag cgatgcggtc gaaagcccgc atctcgcctt ggcgagggcc aaggccgagc
     1141 tctatatcgc ctgcgccgaa atcgacgacc tggccccgtt gccgatggtg gccgagctcg
     1201 agcagctgtt tgccgcggcg ggcaaccccg gcgagctcga aatctatcca ggcgtgcatc
     1261 atggcttcgc ctttccgcag cgcaagatct acgaccaggc ggcggcggag cggcattggg
     1321 agcgcctgat cgcgctctat cggcgccggc tcggctgacg gcgcagccgt gtgcgccgtg
     1381 ccgcagcccg cgcggcgctc atgtcgcgcg cacgaaattg taacggaaac gttcgccgtc
     1441 ggcgctgacg cggccaaccg tcgggttggg aaagtggatc ggtaacagca gggtggaggt
     1501 atcggccacc ttgccgagga actggcgtcg ggcctggtgc gcctgctcct tgtccgtgtc
     1561 gaaaatggtc gaccactgcg gctcgcggca ttgcagcgcg tgatgcatca tgtccccggt
     1621 gaccacggcc acttgtccct gcgaacggat ctcgacgcag caatggtggg gcgaatggcc
     1681 gcgcgtgggg atcagggaga aggtgtcgtc gagctgatag gtatccgcca ccaacagggc
     1741 ctgacccgct tcgacgatcg gccggcaatt gtaggtccag acattgccgg gcgggttggc
     1801 gccgcgctgg gtggcggcct cccaataggc atactcgtcc ttgtggaaaa tatactttgc
     1861 gttcgggaag gtgggcaccc atcgcccgtc gcgcagcatg gtgttccagc cggtatggtc
     1921 gatgtgcagg tgcgtgcaaa agacgtgcgt aatgtcgtcg aaagcgaggc ccgccgcgcg
     1981 gaaattatcg agccagggtt gcttggggaa atccatcggc ggagggtggc ccttgtcttc
     2041 gccggtacag gtatcgatca agacggtgtg cttgggcgtg cgcacgacaa aggtctgata
     2101 ggtaatgacc atcttgccgg aggccggatc gaacaccacg ggatccatct cgacgaggtg
     2161 ccgacggccg atctcggggt cgtaggcagg gaacatatcc tccggctttc gccacggacc
     2221 gtcccgctcg atgatgctgg taatcgtcac ctcaccgatg ttgagcgttc gcatgtgctg
     2281 tactctcccc gttctggcgc cgcgccgata ggctgccgca actgtaaagg cagccaagga
     2341 ctttggaaaa tagcgcatcg cgcaggcggc ccggccgggc cattgcaagc ccgccgcgcg
     2401 cggcggcgcc gtgacggcgg ctgcctgcgg cgccatagag aaagccatcg gcctaacgca
     2461 agggaaaatc gaccatgagt ggcctgctcg aaaaacacat agctgccgtc accggcgcgg
     2521 gttccggaat cgggcgggcg atcgcgctcg gctacggcaa agccggagcc ggggtggtgg
     2581 tgctcgacgc caacgccgag acggcgcaag agacggcgac ggccatcaag agcgagggcg
     2641 gaaaagcctg gagcttccag ctcgatgtcc gggacggcga ggcctgccgc accttggccg
     2701 cggtcgtcgc catcagcgtt ggtcagatct caatcctggt caacaacgcc ggcatcaacc
     2761 gccgcaatgc ttttaccgcc gagccggccg cggtgctcaa ggactggcag gacatcatgg
     2821 ccgtcaacct cgacggcatc ttcaacgtga cgcatgcctt cctgaaagat ctgcggcaga
     2881 gccgcggccg catcatcaac atcggctcga tccagtcctt cgtgcatgtg cgcacgccaa
     2941 actcgccggc ctacaccacc tccaagcacg gggtgttggg tttcacgcgc gcgctggcag
     3001 ccgagctggg caaggacggg gtgcgcgtca acgccatcgg gccggggctg atcgaaaccc
     3061 cgttgaacgc ccaggtgcgc gccaacaatc ccgagctggt gcgaaccttt ctcgagcaca
     3121 ctcccttggc gcgcgccggc aagccggagg acatcgtcgg gcccgccgtc tttctcgcct
     3181 ccgatctgtc ggcttacgtc acgggcgcca tcatcatggt cgacggcggc tatcgcgcca
     3241 tttaggaagg ggcgcggcgc cggaccgcgt tgtagcggcc atcccggcgc gattgcgtcc
     3301 tggcggcgcc aattgaggcc tgcgcgcggg tcccgcgcgc gtcgcaaaaa ccacagctcc
     3361 acacgccttc ggctgcgaag tcacagtgtg accacaatcg gatcgggcaa tagggccggg
     3421 cgggaactcg cgtcgcgccc ggcctgttgt cgggggtgcg cgtgcggcgc aagcgggcgc
     3481 caaacctgct aaccatggac accgtcgcat gaacgttcgg ccgctcttgc acttgctgat
     3541 cagctccctc gtggcggtac tggccgcgct ggtggcgcct cccgccctgg cgcagcagct
     3601 gttctgggat tggggcggtg gcgataccgt cggcgccagc ggccgcgaaa tcgtgcgctt
     3661 cagcgagcgc ttcgcgccgg ggcaagtcgt cgtcagcttc ggcgaccgtc gtctctattt
     3721 cgtgacccgg cccggcgagg cgctgagcta tccaatcgcc attccgcgcg acgaggaccg
     3781 ttgggagggc gtcaccaatg tcaccaataa gcgcgtcaac ccgtcctgga ccccaacgcc
     3841 gaccatgatg gcggaaaacc cgcggctgcc gcgctgggtg cccggtggcc atccgatgaa
     3901 ccccctcggc gtgcgtgcgc tgtacctggg tgccagcgcc tatcgcatcc atggcaccga
     3961 tgcgccgtgg acgatcggca cggcggtatc gaagggatgc atccgcatgt acaaccagga
     4021 cgtgctcgac ctctacccgc gggttgagct cggcgccacg gttacggtga cctgggatcg
     4081 tttccagtcg ggcggcaact atgcctcgac gccgcgcgcg gcggcgccgc gcctaagaga
     4141 gatgcgccaa tcggatctgc cgcgcagctt ttgaggctag cggtcttcct ccggctgcgg
     4201 ggctcggctt ggccgccatt tccgcttcca ctgcttggcg aactggtcgc gctcgcgaat
     4261 gagccgcttg tccgtgcgcg aggcctcgct gctcagcatt tcgctataga gctgcaggcg
     4321 cgccagaccc ttttccattt gcgcaatttt gcccgtcgcc ttcgagactt cccaactgtc
     4381 aggtgacaag tccgccaatt gggcccggtg ccgagccagg cgggtttgca gccgctcgat
     4441 ggcgcgcgcg gtttcttcga ccaggagatt gggggctgcg gccatggcgc ctgcttccgt
     4501 gcttcacccg tgaatcgccg ccaaagcatg tactcgcttg tccggcgcgc caaactccaa
     4561 agtcctgagg atttcctgct gctgtgtgtc tgtagcccgc gcaagggtga cgcgccggcg
     4621 tcgcggagga aatgcacacc tgtccctgcc tgcggccctg ggggtctatg gttaatgcga
     4681 gcgcccgtgc cgacctcggg gagcgatcgg ggcaaggcaa cagggtgtgg gcgaggcctt
     4741 ggcgccaaga gatgccaagc gggcgggcgg cgcggcgcgc gggacccgca ggggccgagt
     4801 cggtgatttg gtcgaggctc aaagctgccg gcgcttttcc accagccgcg cgaatttctc
     4861 gtattcggcc tcgttcacgg tatagctgtg gtcgtcgtcg ggaaatgcgc cctctttgac
     4921 ctccttcaca tattgctgga tgccggcgac cgcgacctcg gtcagcttgg cgaagcgctt
     4981 ggtgaacttc ggcttgaaat cggtgaagac gccgagcaaa tcatgacaga gcaaaatttg
     5041 cccgtccgtg ccgggtccgg caccgatgcc gatggtggga attgtgagct gttcggagat
     5101 cagcttggca attttggcgg gcacggcttc gaattccaac atgaagcagc ccgcatcctc
     5161 gattgccagc gcatcctcca gaattttcat ggccgcctcc gccgtgcggc cttgaatctt
     5221 gaagccgccg aacatggcga tggtatgtgg ggtcagcccg atgtgggacg cggtggggat
     5281 gccggcatcc accagggccc tcagaatatg cgcctggctc ttgccgccct gcggtttgac
     5341 ggcatcggac cccgcttcct gcatgaagcg cgtggcgttc gtgagcgcgc gctcgatggt
     5401 gtggtagctc tggtaaggca tgcagccgag cacgaaggta ttgggagcgc cgcggcggat
     5461 ggcttgggca tgcagcacca tcatgtccat ggtggccggc acggttgagg gatggccgtg
     5521 ggcgatcatg gccaggctgt cgccgacgac ggcgacgtcg acgccggcca tctccgccca
     5581 cttggcgctc gtatagtccg gcaccgacat gtagaccatt ttttcgccgg tctttttgga
     5641 ttggcgaatc tcggtaatgc tgcgcttctc gcgctttttc tgggcgctct tggcatcccc
     5701 cgtcatcggt actcctcctc aggcgatcag cgtttgcgcg atcagagcca ggcaaagcgc
     5761 aaggcccgca aagaatagca aggcgccgcg gccgttcacg aagctgtccg gcaacggccg
     5821 caggatcagg ggcgcggcca gcagcatggc accgccgcca atcgccgccg catagagcgg
     5881 cacgtgtcgc caggcgaagc cggcaagcag cacggtcagg gcggtgcaca gcatcagtga
     5941 gccccaaagc agcacttgcc cggccgtcgt gctgagcgcc ggcgcgcggt attcggccgg
     6001 aaaatgcccg ctcaccgtca agccgtagag cgcgagcacc agcacgagcg ccatgaacac
     6061 ggccaactcg gggagcggag gaagcgccat ggcggtcgac cttttgggtc agcccttatt
     6121 cgtgttcggg cttgttcccg tcgcccgtcc gcggtttgcc gccctgcgcc tcgggaaaga
     6181 cccacattgc caccaccgca aaggcaccga ccccgagcac gagccagaac agctcgggtt
     6241 tgccgttgag cctttcccaa aacgcgaaag cgatggcgac cag
//
LOCUS       DASTMT010000666         3857 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_6478683, whole genome shotgun sequence.
ACCESSION   DASTMT010000666 DASTMT010000000
VERSION     DASTMT010000666.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3857)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3857)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3857
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6478683"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            19..1056
                     /locus_tag="VFR00_15455"
     CDS             19..1056
                     /locus_tag="VFR00_15455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012335363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEU0160718.1"
                     /translation="MKRAATIVLLAALLSLPLWISNLSVLHILIMTGIFIIAAMSLNL
                     LLGFTGQLSLGHVAFFGIGAYTSSLMSLGFEVHLLPSWQVGLDAKPVWLAMLTGVLLA
                     AMCGWVIGRLSFKVRGAYFVIVTVSFAEVVRLVALNWVELTQGPMALNNIPVLRLGIP
                     GYFEFAFLRKPANYYLVLAVGALAYLMIRRLVQSRAGRAMIALRENEALAASVGIDVT
                     RYLVLATFLSAGIAGAAGALYAHYVRIVDPEIFSFIYTVTMVIMVITGGKGTLAGPLV
                     GGLMFGLLPEVLRAFSIPPEIQWVIYGALMILIVYFLPEGIVPALSRAWRGRRQQDDA
                     AALALKTEPSP"
     gene            1053..1835
                     /locus_tag="VFR00_15460"
     CDS             1053..1835
                     /locus_tag="VFR00_15460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020187555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0160719.1"
                     /translation="MSAPGPPDLAVDRLTIRFGGLTAVDGMSFSVRRGEVLSLIGPNG
                     AGKTSAFNAITGYLAPAAGAIVYRGARLNGLKPNQIAELGVVRTFQKTNVFAGLSVLD
                     NVLVGLHLRGTQEPLAILLGAASVAQEERGLAAQAREILQFIGLAARAGQLAAALPYG
                     ELRLLEVAIALAAKPRLLLLDEPVCGLNPAETANFMQLLARIRALDMTVLLVEHDMKM
                     VMGVSDRIICLNQGRIIADGTPTEIRNNAEVVRAYLGTQRPG"
     gene            complement(1882..3240)
                     /locus_tag="VFR00_15465"
     CDS             complement(1882..3240)
                     /locus_tag="VFR00_15465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013949727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEU0160720.1"
                     /translation="MTAAQSHIHQLGEESSVWFKLTPLLKGPHNPLTILMQMAERYGG
                     VIPVNMASQRVVLLTAPEHFKRVLVTKADDYIKYFDGLKPIFGKSMITHDGVLWQKIR
                     MPQQPAFHPDMFAEYIPYFLAAIRSKMETWAKLAQSGETVEMVEQTWTLAADMICKAL
                     FDREMPFNPHFVFKCVKTYTDVMNHKSIRLKKVAGEDFEITEEDAAKAMDVWAGVPPE
                     VFAADPREARERTLLNMIEAAVADPTIPEFDRQQAIDEIKQYLWAGTETTALTLAWAL
                     YETAKNPAAADRIRKEGELVCGDREPTAADYSALAYTRAVIQETMRLYPPIWMLVRVA
                     AKDDVIDGKDVRTGDKVALFAYGAHHNAKFWPEPESFQPERWLGDAAKKRIPYTYLPF
                     GGGKRSCIGGAMSQVENTLALSILLRRFRPEYVGPEPPGINATVTLTPKGGLPFKIRE
                     LS"
     gene            complement(3453..>3857)
                     /gene="purN"
                     /locus_tag="VFR00_15470"
     CDS             complement(3453..>3857)
                     /gene="purN"
                     /locus_tag="VFR00_15470"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019857369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEU0160721.1"
                     /translation="DVELIALAGFMRIQSATFVRKWHGRQLNIHPSLLPAFKGLNPHA
                     QALQAGVKISGCTVHFVSEELDGGPIIAQTAVPVCEGDSAESLAARILVAEHALYPEA
                     LALVATGRTQLNEGRVVVLPAGSTAAARPAQP"
BASE COUNT          612 a         1235 c         1247 g          763 t
ORIGIN      
        1 gcggcgggag cggatcgggt gaagcgcgcg gcaacgatcg tcttgctggc agcgcttctc
       61 tccctgccgc tgtggatctc gaacctctct gtcctgcata tcctgatcat gaccggcatt
      121 tttatcatcg ccgccatgag cctcaatctg ttgctgggtt tcacgggcca gctgagcctc
      181 ggacacgtcg cctttttcgg gatcggcgcc tacacgtcga gcctcatgtc gctgggcttc
      241 gaggtgcacc tcttgccaag ctggcaggtg gggctcgacg ccaagcccgt ttggctggcc
      301 atgctgacag gcgtcctgct ggcggcgatg tgcggctggg tgatcggccg cctctccttc
      361 aaggtacgcg gcgcgtattt cgtgatcgtc acggtgagct ttgccgaggt cgtacgcctc
      421 gtggcactca attgggtgga gctgacgcag ggcccgatgg cgctcaacaa cattccggtg
      481 ctgcggctcg gcatccccgg ctatttcgag ttcgcctttc tgcgcaagcc ggcaaactac
      541 taccttgtgc tggcggtcgg cgccctcgcc tacctcatga tccggcggct ggtgcagtcg
      601 cgcgcgggcc gcgccatgat tgcgctcagg gaaaacgagg cgctggccgc ttccgtcggc
      661 atcgacgtga cgcgctacct ggtgctcgct accttcctct ccgccggcat cgccggcgca
      721 gccggcgcgc tttacgccca ttacgtgcgc atcgtcgatc cggagatctt ctcgttcatc
      781 tatacggtga cgatggtcat catggtcatc accggcggca agggcactct ggccgggcct
      841 ctggtgggtg gcctgatgtt cgggctgttg ccggaagttt tgcgggcctt cagcatcccg
      901 cccgagatcc agtgggtgat ttacggggcc ctgatgatcc tcattgtcta cttcctaccg
      961 gagggcatcg tgcccgcgct cagccgcgcc tggcgcggac ggcgccagca agacgacgcg
     1021 gcggccctcg cgctcaagac cgagccatcg ccatgagcgc gcctggcccc cccgacctgg
     1081 cagtcgaccg cctgacgatc cgcttcggcg gtttgaccgc cgtcgacggc atgtccttca
     1141 gcgtgcgccg tggcgaagtg ctctcgttga tcgggccgaa tggtgccggc aagacttccg
     1201 ccttcaatgc cattaccggc tatctcgcgc cggccgcggg cgcgatcgtc tatcgcggtg
     1261 ctcggctcaa tggcctcaag cccaaccaaa tcgccgagct cggcgtggtc cggaccttcc
     1321 agaagaccaa cgtgttcgcg gggcttagcg tgctcgacaa cgtgctggtc gggctgcatt
     1381 tgcgcggcac gcaagagccg ctggcgatct tgctgggtgc tgccagcgtc gcccaggagg
     1441 agcgcggcct cgccgcccag gccagggaga ttttgcagtt catcggcctt gccgcgcgcg
     1501 cgggccagct tgcggccgcg ctgccctacg gcgaactgcg cctcctagag gtcgccatcg
     1561 cgctggcggc caaaccgcgg ctgctgctct tggacgagcc cgtatgcggg ctcaatccgg
     1621 ccgagaccgc caacttcatg cagctccttg cgcgcattcg cgcgctcgac atgaccgtgc
     1681 tgctcgtcga gcacgatatg aagatggtga tgggcgtgtc cgatcgcatc atctgcctca
     1741 atcagggccg catcatcgcc gacggcaccc ccaccgagat ccgcaacaac gccgaggtgg
     1801 tgcgcgccta tcttggcacg cagcgaccgg gttagctgag gatcagccaa cgcaggccgg
     1861 ccttgcggtc cgccgcggcc gtcaactcag ctcgcgaatc ttgaacggca ggccgccctt
     1921 cggggtcagc gtcacggtgg cattgatgcc gggcggctca gggcccacgt actcgggacg
     1981 aaagcggcgc agcaggatcg atagggccaa agtgttttcg acctggctca tggcgccgcc
     2041 gatgcaggag cgcttgcccc cgccgaacgg caggtaggta taggggatgc gtttcttggc
     2101 cgcgtcgccc agccagcgct cgggctggaa ggactccggc tcgggccaaa acttggcgtt
     2161 atggtgcgcg ccgtaggcga acagagccac cttgtcgccc gtgcgcacat ccttgccgtc
     2221 gatgacgtca tccttggcgg caacgcggac cagcatccaa atcggcggat agagacgcat
     2281 cgtctcttga atcaccgcgc gcgtgtaggc gagtgcggag tagtcggcgg ccgtcggctc
     2341 ccgatcgccg cacacgagct cgccttcctt gcggatacga tcggccgcgg cggggttctt
     2401 tgccgtctcg tagagcgccc aagcgagcgt cagcgccgtg gtctccgtgc cggcccacag
     2461 gtattgcttg atctcatcga tcgcctgctg gcgatcgaac tcgggaatgg tggggtcggc
     2521 cacggcggct tcgatcatat tcaagagcgt gcgttcgcgc gcctcgcgcg ggtcggcggc
     2581 gaatacctcc ggtggcacgc ccgcccacac gtccatcgcc ttggccgcat cctcttcggt
     2641 gatctcgaag tcctcaccgg ccaccttctt gaggcggatc gacttgtggt tcatcacgtc
     2701 ggtgtaggtc ttcacgcact tgaagacgaa gtgtggattg aagggcattt cgcggtcgaa
     2761 cagggccttg cagatcatgt ctgcggccaa tgtccaggtc tgctcgacca tctccaccgt
     2821 ctcgccgctc tgggcgagct tggcccaggt ctccattttg ctgcggatgg cggcgaggaa
     2881 gtagggaatg tattcggcaa acatgtccgg gtggaacgcc ggttgttgcg gcatgcggat
     2941 cttctgccac agcacgccgt cgtgcgtgat catggacttg ccgaagatgg gtttcaggcc
     3001 gtcgaaatac ttgatgtaat cgtcggcctt ggtgacgagc acgcgcttga aatgctcagg
     3061 cgccgtcaac agcacgacgc gctggctcgc catattgacg ggaatgacgc cgccgtagcg
     3121 ctccgccatc tgcatcagga tcgtcaaagg gttgtgtggg cccttgagca gcggagtcag
     3181 cttgaaccag accgaggatt cttcgccaag ctggtggata tgggactgag cggcggtcat
     3241 cttaatgcgc tccggaggcg gtggcggcgg caccaaaaca ttaaatcaac cccgcacctg
     3301 ctcgttccgc cccgggccgt tgtcgctccc ctgtgccatg aggttcgcat ggtatttttg
     3361 gtctcgtagc accgccatca aaactctgta aagtttccgc actgcggcca atgcgctttt
     3421 ggtggaaagg tgtggccacg attaaccaaa cgctaaggtt gcgctggtct tgccgccgcg
     3481 gttgacccgg cggggagcac aacaacgcgg ccctcgttga gctgggtgcg gccggtggca
     3541 accagtgcca gggcctcggg ataaagggcg tgttcggcca cgaggatgcg ggcggcgagc
     3601 gattcggcgc tgtcgccctc gcacacgggc accgccgtct gggcgatgat ggggccgccg
     3661 tcgagctcct cgctgacgaa atgcaccgtg caaccggaaa tcttgacccc tgcctgcagc
     3721 gcctgggcgt gcgggttcaa gcccttgaag gccggcagca acgagggatg gatgttgagc
     3781 tgtcgtccat gccacttgcg cacgaaggtg gcgctctgga tgcgcatgaa acccgccaag
     3841 gcaatcagct cgacatc
//
LOCUS       DASTMT010000667         6279 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_3926006, whole genome shotgun sequence.
ACCESSION   DASTMT010000667 DASTMT010000000
VERSION     DASTMT010000667.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 6279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6279)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6279
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3926006"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..160)
                     /locus_tag="VFR00_15475"
     CDS             complement(<1..160)
                     /locus_tag="VFR00_15475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009491505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU0160722.1"
                     /translation="MRSDPITCDVLIAGSGAAGFATALTAQLEGLDVIMVEKEPLFGG
                     TTAYSAGVV"
     gene            complement(193..753)
                     /locus_tag="VFR00_15480"
     CDS             complement(193..753)
                     /locus_tag="VFR00_15480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006020168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEU0160723.1"
                     /translation="MVALALQCTLTWAARAESFAERVAPCLACHGGEGQSSLPDVPSL
                     GAQPAPYTLIQLYLFRGRQRRVEVMNEATKDFSDDDLKTFSDFIATLPAPRPTPGEAA
                     EQGRIERGQALVRQHHCDFCHNPDLAGRDNVPRLAGQREDYLIRSLSEYKANTRPGYD
                     ASMADVLARVTEGEILDLAYFLARQP"
     gene            complement(842..2125)
                     /locus_tag="VFR00_15485"
     CDS             complement(842..2125)
                     /locus_tag="VFR00_15485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018273075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEU0160724.1"
                     /translation="MRKILVVSGFCTAAALAASAVPGAHAQQTPAAPAATPAAPAATP
                     QPLPPGSPLLGRPDTEAAKKLAPVAPPPVPTAADKLPLDKLKVKTGFKIEVYAAGVPN
                     ARSLKLGPKGTVFVSSRLQDKVHAIVEKNGKREVKVIASGLHRPNGIVVHKGTLYIAE
                     LSQISKIDNIEDNLDNPPKPTVILSDLPKDEAHGWKYLTVGPDEKLYFHIGAPCNICM
                     PPERHAKIYRVGLDGKDLEVYATGVRHIVGMDWHPTLHQLYFSENARDWLSEDMPEDK
                     LNRVTKPGKDNFGYPYCHQGNLPDPEFGWGRSCSEFTKPLTLLGAHAASLGLKFYTGK
                     GFGSEYQNAIFLARHGSWNKSVKSGGDVMAIKLNRDGTVKSKESFITGFIQNNNYVGR
                     PVDVLPMPDGSMLVSDDFNGAVYRVSRGSNRVAGK"
     gene            2391..3107
                     /gene="rplS"
                     /locus_tag="VFR00_15490"
     CDS             2391..3107
                     /gene="rplS"
                     /locus_tag="VFR00_15490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013417949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEU0160725.1"
                     /translation="MTAILELEREQISAILAKRSVPEFSPGDTVKVMVKVIEGMEVDP
                     KDKKKAKPEEPKGPGFRLQAYEGVVIARSGSGINESFTVRKISYGEGVERVFPIYSPY
                     ITEIEVLRRGKVRRAKLYYLRGRRGKSARIFERTDARAKRLNAAFKGFKRPKGEADDL
                     TRIKGVDAELAARLNQLGAIKFEQIANFSDDDIANLDEALSLAGRIEKDDWVGQAQSL
                     AAESAAAEVPVAEEEKAAKK"
     gene            3183..3854
                     /locus_tag="VFR00_15495"
     CDS             3183..3854
                     /locus_tag="VFR00_15495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013168513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HEU0160726.1"
                     /translation="MRRGVIFADRAEAGRLLAARLQELKLASPVVFALPRGGVPIGAE
                     VARAMAAPLDVAFARKIGAPQQPELAVGAVAGAPGAPELVLNSALIAALDLSEEFIAQ
                     SVARELASIERQRAQYRAVRPAIDMANSTAIVVDDGVATGMTTTAALRHLRARQPRRL
                     IAAAPVAAAEAAALLQTEADEVVLISAPRRFGSVGGFYRSFTQVSEAEAMALLSAANG
                     KDVTV"
     gene            complement(3962..4612)
                     /locus_tag="VFR00_15500"
     CDS             complement(3962..4612)
                     /locus_tag="VFR00_15500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006727190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018784 - (S)-2-haloacid dehalogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase type II"
                     /protein_id="HEU0160727.1"
                     /translation="MHAYVFDAYGTLFNLDAAAERHQAAIGPGWRHFSQTWRSKHIEY
                     TWHHTLTRTPATFWRLAERSLEFAIAATGIGIGRDVRAGLLAAYREMAAYPEVAEVMR
                     ALKSRGATLAILSNGDPDMLADAVSAAGLGDVFDAHLSVAAAGAFKPAQRVYQLASDH
                     FGCRAEEISFQSSNRWDIAGAKAFGFYCVWINRSGAPDEYPELPPDRVVGDLRGIL"
     gene            complement(4615..5505)
                     /gene="mmsB"
                     /locus_tag="VFR00_15505"
     CDS             complement(4615..5505)
                     /gene="mmsB"
                     /locus_tag="VFR00_15505"
                     /EC_number="1.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012320715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008442 - 3-hydroxyisobutyrate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006574 - valine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyisobutyrate dehydrogenase"
                     /protein_id="HEU0160728.1"
                     /translation="MARIGFIGLGNMGLPMAGNLVKAGHAVAGFDLLGENLERAAGRG
                     VNPERSAPDAAKDAEIVVTMLPAGKQVLEVYQAGVLGAARANTLFIDSSTIDVASARA
                     AHQLATQAGMLGLDAPVSGGVGGAEAASLTFMAGGTAAAFAQAKPILESMGRRVIHCG
                     DAGAGQAAKICNNMMLGINMIGVSEAFTLAEKLGLSPKALFDVAAASSGQSWALTSYC
                     PVPGLVPAAPANNNYKPGFASALMLKDLRLAQEAAAVAGASTPLGAAAAQLFGLHNAW
                     GEGGTDFSGIIHLLRGRGGQ"
     gene            complement(5572..>6279)
                     /locus_tag="VFR00_15510"
     CDS             complement(5572..>6279)
                     /locus_tag="VFR00_15510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015684290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU0160729.1"
                     /translation="RTLLQSIVSPVISTSLYFVVFGAAIGSRISEIEGVSYGAFIVPG
                     LIMMMLLTQSTANASFGIYFPKFVGTIYELLSAPISYLEIVLGYVGAAATKSIILGLI
                     ILLTAGFFVPIRIDHPLAMIAFLLLTAVSFSLFGFIIGVWADGFEKLQVIPLLIITPL
                     TFLGGSFYSIHVLPPVWQAISMFNPILYLISGFRWSFYSIADVSVGLSLAAAVLFTAL
                     CLALVWWIFRTGYRLKN"
BASE COUNT         1158 a         1945 c         2116 g         1060 t
ORIGIN      
        1 acaccacgcc tgccgagtag gccgtggtgc cgccgaacag tggctccttc tcgaccatga
       61 tgacgtcgag gccttccaac tgcgccgtca aggcggtggc aaacccagcc gcgcccgagc
      121 cggcaatcag cacgtcgcag gtgatgggat cgctacgcaa cgggcgctca ttgttaggag
      181 ctggtgggca agttagggct ggcgggcaag aaaataggca agatccagga tctccccctc
      241 cgtgacgcgc gccagaacat cggccatcga cgcgtcgtag ccgggacggg tattggcttt
      301 atactcgctc agcgatctga tcagatagtc ctcgcgttgc cccgccaggc gcgggacgtt
      361 gtcgcgaccg gcgagatcgg ggttgtgaca gaaatcgcag tgatgttggc gcaccagggc
      421 ctggccgcgc tcgatgcgcc cttgctcggc ggcttcgccc ggcgtcggtc taggggcggg
      481 cagcgtggcg atgaagtcgg aaaaggtctt aaggtcgtcg tcagaaaaat ccttggtggc
      541 ctcgttcatc acctcgacgc ggcgctggcg accgcggaag agataaagtt ggatcagtgt
      601 ataaggtgcg ggctgggcgc cgagcgaggg cacatcgggc aagctcgatt gaccctcgcc
      661 gccatggcag gccaggcagg gggcaacacg ctcggcaaag ctttccgccc gtgccgccca
      721 ggtcagggtg cattgcaggg cgagggcgac caccaccacc ccgacgcgga ggccaacacg
      781 atcgcggtgg cgtgtggcga ggtcgggagc ggagcgcccc cgaccctgtg ttgcaggcga
      841 attatttgcc ggcaacgcga ttgcttcccc ggctgacgcg atagaccgcg ccattgaagt
      901 cgtcggaaac gagcatcgaa ccatcgggca tcggcagcac gtcgacggga cggcccacgt
      961 agttgttgtt ctggatgaag ccggtgatga aactctcctt ggactttacc gtgccatcgc
     1021 ggttgagctt gatggccatg acatcgccgc cgcttttcac gctcttgttc cacgagccgt
     1081 gccgggccag gaaaatggca ttctgatact ccgagccgaa acccttgccg gtatagaact
     1141 tgagccccaa ggacgccgcg tgggcgccca agagggtgag cggcttagta aactcgctgc
     1201 aggagcgtcc ccaaccaaac tccgggtcgg gcaaattgcc ctggtggcag tagggatagc
     1261 cgaagttgtc cttgcccggc ttggtgacgc ggttgagctt gtcctcgggc atgtcctccg
     1321 acagccaatc acgcgcgttc tcagaaaaat agagttgatg cagggtcgga tgccagtcca
     1381 tgccgacgat gtggcgcaca cccgtagcgt agacctcaag atccttgcca tcgagcccga
     1441 cgcgataaat cttggcgtgc cgctcggggg gcatgcagat gttgcagggt gcaccgatgt
     1501 ggaaatagag cttctcatcg ggcccgacgg tcagatattt ccaaccgtgt gcctcgtctt
     1561 tcggcaggtc gctcagaatg acggtgggct tcggcgggtt gtcgaggttg tcctcgatgt
     1621 tgtcgatctt ggagatctgc gacaattcgg cgatgtaaag cgttcccttg tggacgacga
     1681 tgccgttggg gcggtgcaac cccgaggcaa tgaccttcac ctcgcgtttg ccgttctttt
     1741 cgacaatggc atgcaccttg tcctgcaaac gactgctgac aaacaccgtc cccttggggc
     1801 caagcttcag cgagcgcgca ttgggcactc ccgccgcata gacctcgatc ttgaagccgg
     1861 tcttaacctt gagtttgtcg aggggcagct tgtcggccgc cgtgggcacc ggcgggggcg
     1921 caacgggtgc caattttttg gcagcctccg tgtccggtcg gccgagcagc ggcgagcccg
     1981 gcgggagggg ctggggcgtg gcggccggag cggctggcgt ggcggccggt gcggctggcg
     2041 tttgttgggc gtgtgcgccc ggcaccgcgc tggccgcaag cgcggcagcg gtacaaaaac
     2101 ccgaaacgac gaggatctta cgcattagcg cctccctgaa ctcatcaaac cgcttctggg
     2161 ccactggctc gacggcggca cacaactcta ttcgcctggt cagtttctgc caacggttag
     2221 gctgcccggc cgtcgcgccg agggcaggga ggcaaaaaac gcccgatcgc ccatcgacaa
     2281 gcccggcggt tctgggctag aacgcggccg atttccattc gacgcgcccg gtcgtcggcc
     2341 gcccatgcga agggtcgggc tgggagccat tgacttgaat cgaggacccc atgaccgcca
     2401 ttttggagct cgagcgcgag cagatcagcg ccatcctcgc caaacgctcc gttcccgagt
     2461 tcagccccgg ggataccgtc aaggtgatgg tcaaagtcat cgaaggtatg gaggtcgacc
     2521 ccaaggacaa gaaaaaggcc aagcccgagg agcccaaggg gcccggcttc cgcttgcagg
     2581 cctatgaggg cgtggtgatt gcgcgctcgg gcagcggcat caacgaaagt ttcacggtgc
     2641 gcaagatttc ctacggcgag ggtgtggagc gcgtctttcc catctactcc ccctacatca
     2701 ccgagatcga ggttttgcgc cgcggcaagg tgcgccgcgc gaagctctat tatttgcggg
     2761 ggcggcgagg caaatcggcg cgtatctttg agcgcaccga cgcgcgcgcc aagcggctga
     2821 acgcggcctt caagggtttc aagcggccga agggtgaagc cgacgatctc accaggatca
     2881 aaggcgtgga cgccgagctc gccgcccgcc tcaatcagct cggcgccatc aagttcgagc
     2941 aaatcgccaa cttctccgac gatgacatcg ccaatctcga cgaggccctc tcgcttgccg
     3001 ggcgcatcga gaaggatgat tgggtcggtc aggcacaaag cctggcggcc gagtcggccg
     3061 ccgcggaagt gcccgtggcc gaggaggaga aggccgccaa gaagtaggcc gcggcgccgg
     3121 cttgccatgc gcgcccgggc ctaacttgcg ctaagatcgt gcggtggggg cagagagccc
     3181 gcatgcgccg aggagtgata ttcgccgatc gcgccgaagc cggccgactg ctggcggcac
     3241 ggctgcaaga gctgaagctc gcctccccgg ttgtcttcgc cttgccgcgt gggggcgtgc
     3301 cgatcggagc cgaggtggct cgggcgatgg cggcgcctct cgacgttgcc tttgcgcgca
     3361 agatcggggc cccacagcaa ccggagctcg ccgtcggcgc ggtggccggc gcgccgggcg
     3421 cgcccgaact cgtgctcaat agcgcgctga tcgccgctct cgatttaagc gaggagttca
     3481 ttgcgcaaag cgtggcgcgc gagctggcga gcatcgagcg ccagcgcgcc cagtatcgtg
     3541 ccgtacgccc ggctatcgat atggcgaaca gcaccgccat tgtggttgac gacggcgtgg
     3601 cgacgggcat gacgactacg gccgcgctgc gacatctgcg ggccaggcag ccgcgccggt
     3661 tgatcgcggc ggcgccggtg gccgcggccg aggccgccgc gctgttgcaa acggaggccg
     3721 acgaagtggt gctgatcagc gcgccgcgcc gctttggttc ggtgggcggc ttttatcgca
     3781 gctttacgca ggtgagcgag gcggaagcga tggctcttct cagcgccgcg aacggcaagg
     3841 acgtgacggt ctaggtcgcc attcccttgg gtgcggcccg gggcttgcgg ccttgcggcg
     3901 acagcgtgag cggcggtgcg cccgaggccg tcgccggggg cgttgcgccc cgcgccggcc
     3961 ctcacagaat gccgcgcaaa tcgcccacca cgcggtccgg gggaagctcg ggatattcgt
     4021 cgggcgcccc gctccggttg atccagacgc agtagaagcc gaaggctttg gccccggcga
     4081 tatcccagcg gttggacgac tggaaagaga tctcttctgc gcggcagccg aaatgatcgc
     4141 tggccagctg gtagacgcgt tgtgcgggct tgaaagcgcc ggccgccgcc accgataggt
     4201 gggcatcgaa cacatcgccg aggccggcgg ccgagacggc gtcggccagc atgtcggggt
     4261 cgccgttcga caggatcgcc agggtcgcgc ccctgctctt gagcgcgcgc atgacctcgg
     4321 ccacctcggg gtaggcggcc atctcgcggt aggccgctaa gagcccggcg cgaacgtcgc
     4381 ggccaatgcc gatgccggtc gcggcaatgg caaactccag gctgcgctcg gccagccgcc
     4441 agaacgtcgc aggcgtccgg gtcagggtgt ggtgccaggt gtattcgatg tgcttgctgc
     4501 gccaggtctg ggagaagtgc cgccagcctg ggccgatggc ggcctggtga cgctcggccg
     4561 ccgcgtcgag attgaagagc gtcccatagg catcgaacac gtaggcgtgc atggctattg
     4621 gccgcctctg ccgcgaagga ggtgaatgat gcccgagaag tcggtacccc cctcgcccca
     4681 ggcattgtgc aggccgaaca actgtgccgc cgccgcgccc aacggcgtgc tagcgccggc
     4741 cacggcggcc gcctcttgcg caagcctcaa atccttgagc atcagcgcgc tggcaaagcc
     4801 cggtttgtag ttgttgttgg cgggtgccgc cgggaccaag cccggaaccg gacagtagct
     4861 ggtcaacgcc caggattggc ccgaggaggc ggcggcaaca tcgaacaggg ccttaggcga
     4921 aaggccaagc ttttcggcca gcgtgaaggc ctcgctgacg ccgatcatgt tgatgccaag
     4981 catcatatta ttgcagatct tggcggcctg gccggcgccg gcatccccgc aatggatgac
     5041 gcgtcggccc atgctttcca ggatcggctt cgcctgcgcg aaggccgccg ccgtgccccc
     5101 ggccatgaag gtgaggcttg ccgcctccgc gccgccgacg ccgcctgaga ccggggcatc
     5161 aaggccaagc atgcccgcct gcgtggcaag ctgatgcgcc gcgcgggcgg acgccacgtc
     5221 gatcgtggat gaatcgatga acagcgtgtt ggctcgcgcc gcccccagca cgccggcttg
     5281 atagacctcg agcacctgct tgccggccgg cagcatggtg acgacgattt cagcgtcttt
     5341 ggccgcatcc ggcgcggagc gctcgggatt gacgccgcgg ccggcggcgc gctccaggtt
     5401 ctcgccgagg aggtcgaagc cggccaccgc atggccggct ttgaccagat tgcccgccat
     5461 cggcaagccc atgttgccca agccgatgaa gccgatcctc gccatgggat cctcccttgt
     5521 ccgtaaaatg acgttacgca gcttgctttg cgcccgccag cgcgcccttg cctaattctt
     5581 cagccgatag ccggtgcgga agatccacca taccagggcc agacacaggg cggtgaagag
     5641 caccgccgcg gccaggctga ggccgacgct gacatcggcg atactgtaga agctccaacg
     5701 aaagccgctg atcaaataga ggatggggtt gaacatactg atggcctgcc aaaccggtgg
     5761 cagcacgtgg atcgaataaa agctgccgcc cagaaaggtg agcggcgtga tgatgaggag
     5821 ggggatcacc tgcagctttt caaagccatc cgcccagacc ccgatgatga agccgaacag
     5881 gctgaagctg accgccgtca ggagcaaaaa ggcgatcatc gccaagggat ggtcgatgcg
     5941 tatgggcacg aaaaaacccg ccgtcagcag gatgataagc cccaagatga tggacttggt
     6001 cgccgccgca ccaacgtagc ccagcacgat ttcgagataa gagatgggtg ccgatagcaa
     6061 ctcgtagatg gtgcctacga acttgggaaa atagataccg aaagaggcat tggcggtgct
     6121 ctgcgtcaag agcatcatca tgatgaggcc cggcacgatg aaggcgccat agctcacgcc
     6181 ttcgatttcg ctgatgcgcg agccaatcgc cgcgccgaac accacgaagt aaagcgacgt
     6241 tgaaatgacc ggtgagacga tgctctgcag cagcgtgcg
//
LOCUS       DASTMT010000668         1587 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_5823576, whole genome shotgun sequence.
ACCESSION   DASTMT010000668 DASTMT010000000
VERSION     DASTMT010000668.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 1587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1587
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5823576"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..861
                     /locus_tag="VFR00_15515"
     CDS             <1..861
                     /locus_tag="VFR00_15515"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEU0160730.1"
                     /translation="PPSIPLVVYAILTEQNIAKLFLAAFIPAGLAVIGFFIAIRAYIY
                     LYPDAGPATPKLPFFERARRLGETWPVLAIFLIVFVGMNGDFFFGRSLFTPTEGAAVG
                     ALLATIAALTVGGMRLRQVLASVRATAEQSGMIFLILLGAELYNAFLARTNMPQIAAQ
                     WVAGLGISPFLVMAVIVLVYIALGCLMDSLSMILLTVPIFFPVVMGLNFGLSPEHTAI
                     WFGILALIVVELGLIHPPVGLNVYVIQNIAGDVSMLETFKGVVPFLIAEFFRVSLIIA
                     FPVLTLILVR"
     gene            994..>1587
                     /locus_tag="VFR00_15520"
     CDS             994..>1587
                     /locus_tag="VFR00_15520"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HEU0160731.1"
                     /translation="MAEVERAWLGEVPPMRSERHYGNRVMRCFVERPGNAFELLSAAA
                     ARNGDGEAIVCGAERLSYRQVLATVEACAAGLAAAGIGKSDRVAMLLGNGIAFPLVLF
                     ATLRLGAIAVPLSTREQTQGLAYMLQHAGAKLLVYDAELAERLPPQDATPALIARVAV
                     AAEASYRGLRLLAEPTGAAPAAVEEEDTAIILYTSGTT"
BASE COUNT          204 a          526 c          524 g          333 t
ORIGIN      
        1 ccgccctcga tccccctcgt cgtctacgcc attctgaccg agcagaacat cgccaagctg
       61 tttctggcgg ccttcatccc cgcggggctg gcggtgatcg gctttttcat cgccatccgc
      121 gcctacattt acctttatcc cgatgccgga ccggccaccc ccaagctgcc gtttttcgaa
      181 cgcgcgcggc gcctgggcga aacctggccg gtgctcgcta tttttctcat cgtcttcgtc
      241 ggcatgaacg gcgacttctt tttcgggcgc tcgctgttca cgccgaccga aggggcggcc
      301 gtgggtgccc tgctggcaac cattgcggcg ctgaccgtcg gcggcatgcg tttgcggcaa
      361 gtgttggcct cggtgcgcgc caccgccgag cagtccggca tgatcttcct gattttgttg
      421 ggggccgagc tctataacgc ctttctggcg cgcaccaaca tgccccagat cgccgcccag
      481 tgggtggcgg ggctcggcat ttcgccgttc ctggtgatgg ccgtgatcgt gctggtctac
      541 atcgccttgg gatgcctgat ggacagcctc tcgatgatcc tgctgacggt gccgattttc
      601 tttcccgtgg tgatggggct caacttcggg ctgagcccgg agcacaccgc gatttggttc
      661 ggcatcctgg cgctgatcgt ggtcgagctc gggttgatcc atcccccggt cgggctcaac
      721 gtctatgtga tccagaatat cgccggtgac gtctccatgc tcgagacctt caagggcgtg
      781 gtgccgtttc tgatcgccga gttcttccgg gtctcgctga ttatcgcctt tcccgtgctg
      841 acgctgattt tggtgcgctg atgcggcggc gtgcgctggg cgcccaggcc gtggccgaac
      901 ccggcatgac ggcgcgggcg gggggtgcgc gtagcacggg aatggtgttt gcttgcaagg
      961 cgccgcggcg ggttcggcaa gaggaggctg gaggtggctg aggtggaacg ggcttggctc
     1021 ggcgaggtgc cgccgatgcg aagcgaacgg cactatggga accgcgtcat gcgctgcttc
     1081 gtcgagcgcc ccggcaatgc ctttgagctt ctgagtgcgg ccgccgcgcg caacggtgac
     1141 ggggaggcga tcgtctgcgg cgcggagcgt ctttcctatc ggcaagtctt ggcgacggtg
     1201 gaggcctgcg cggccgggtt ggccgcggcc gggatcggca agtccgaccg cgtcgccatg
     1261 ctgctcggca acggcatcgc ctttcccctc gtcctctttg ccaccctgcg gctcggcgcc
     1321 atcgccgtgc cgctctcgac gcgcgagcaa acccaggggc tcgcctatat gctgcagcac
     1381 gccggcgcca agctcctggt ttacgacgcc gagctcgccg agcgtctccc cccacaggat
     1441 gcaacgcccg ccctcatcgc gcgcgtggcc gtcgccgcgg aggcttctta tcgcggcttg
     1501 cgcttgctgg ccgagcccac cggcgccgcg ccggccgcgg tcgaggaaga ggataccgcc
     1561 attattcttt atacctcggg caccacc
//
LOCUS       DASTMT010000669         2837 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_6483420, whole genome shotgun sequence.
ACCESSION   DASTMT010000669 DASTMT010000000
VERSION     DASTMT010000669.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2837)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2837)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2837
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6483420"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..194)
                     /locus_tag="VFR00_15525"
     CDS             complement(<1..194)
                     /locus_tag="VFR00_15525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEU0160732.1"
                     /translation="MSTRITLLAFMVLTLSASAQAKNREARESDDFSRPVSGPPVMAI
                     VALSEQHVTIYDAEGKLLRAP"
     gene            1138..2280
                     /locus_tag="VFR00_15530"
     CDS             1138..2280
                     /locus_tag="VFR00_15530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018445031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEU0160733.1"
                     /translation="MQRQPEIAIIGAGIGGLALAGLLSRQGAHVRLFEQATAFERIGA
                     GIQMSPNAMHVLRALGLEPLLRQLAFEPPAWTHRVWDTGEHLADLSFGAEAERRYGAP
                     YLLLHRGDLHAAILSAVPLDVIQFDKKLVDLARRGSGVELRFADGSRALADGVVGADG
                     VHSKVREILLGPERPIFTGRVAHRTVFPAGLLGEFAVDTCTKWWGPDRHIVVYPVNPR
                     RDEIYFVTSVPDPHWDVESWSARGEMAEVQNALAGFHPDVQRLLAACPQVHKWALFER
                     DPLPRWREGPVVLLGDACHPMTPYMAQGAANALEDAAVLSRCLAGTVDPALAFARYEA
                     TRLQRTARIQLTSRQNTWGKTAGDPSWVYGYNAWATPIAEAPGLPA"
     gene            complement(2287..>2837)
                     /locus_tag="VFR00_15535"
     CDS             complement(2287..>2837)
                     /locus_tag="VFR00_15535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiB"
                     /protein_id="HEU0160734.1"
                     /translation="YVPPHQPVELFAQAMRAIGEPIHGRAASEISIADLLGQLFAYTE
                     VFEMKTRPELLLLQKTMVVVEGVARSLDPELNIWNAAEPIAREWVETNYGVAGRLKGA
                     GEGAELLGKVVAEVPRLLEQGERVAQALAETAQAGLRLDDETIERLAAAQARHDRWSR
                     WAMWATAAALLAIAAWLLSPLG"
BASE COUNT          453 a          969 c          901 g          514 t
ORIGIN      
        1 ggggcgcgca acagcttgcc ctccgcgtcg tagatggtga cgtgctgctc gctcaacgcc
       61 acgatcgcca tgaccggggg gccactgacg gggcgggaaa aatcgtctga ctctcttgcc
      121 tcgcggttct tcgcctgagc ggaggcactg agcgttaaca ccatgaacgc cagcagggta
      181 atacgcgtgg acacggcacc tactcgtttg acgaactgtt tcatttggcc ttgccgatga
      241 agctgagaaa agagacgcgt cccgcgcgcc atttcattag cgaacctaga ttaaagaggc
      301 ttgagcgtcg ccgggttccc gcgggtcgtg gttagcgcca acaccatccc ggaggattgc
      361 gccgcaagga cggcaatgct cgagaaaatg tcggccatac aagtcgcata gctgccccac
      421 gccaatgtgt acccctgttg catcatctct cctccatttc gtcctcggtt tggcaatgcc
      481 tggcaccgcg tccggtttcc gcaaacccat gaccattcag tagctacggt gctggggtgg
      541 ctcggttgcg cggccgcgcc gaaatgcgcg acctcccgca catcggccgc tcgtggcagc
      601 gtcgtcgcgg cgacaacacc ttcgaccttg ctgcccgatc ccttccaagc cagcgcggcc
      661 cggcctcgtt gaggggcgca cgaaactcat gtctgctgcg cccgcgggct gaggtccggc
      721 atgggcccgg ctgcgatcgg cgtactgccc aacgtggtgc cctttcccgc cgccaggcag
      781 cgtggtggga gccgatttgc gcgcggtcat tcttgtccct cgagcaagac cctccgcatc
      841 ttctgttgtg ctcgggaggg ttattaacaa tgatcaagct caagcatcta acccatgatg
      901 cttcgggggg ttccatccgg ccattcctgg acgatcgagg gtggtgattc cggacaagga
      961 ccactgctgg tgcgctcggc cgcaggcgca cgtccgttcg agctcggcct aagagccaac
     1021 gcattacgcg tggtcaacgc cggcgacggc gcatgatgca tccggagttg gagaacgcgt
     1081 ggcttgacgc tgcgcgcgaa agtgctgatc gttccgcggg gttgtaggga ctagcacatg
     1141 caacgtcagc ctgagattgc cattatcggc gccggcatcg ggggcctcgc acttgccggt
     1201 cttttgtccc gccagggcgc gcatgtcagg ctcttcgagc aggcgacggc gtttgagcgc
     1261 attggcgccg gcatccagat gagtcccaat gccatgcatg tactgcgggc cttagggctc
     1321 gagcccctct tacgccagct cgccttcgag ccgcccgcct ggacccaccg cgtttgggat
     1381 acgggcgagc acctcgccga cctcagcttc ggcgccgagg ccgaacggcg ctatggcgca
     1441 ccctacctgt tgctgcatcg tggtgacctg cacgccgcca ttctctccgc cgtcccgctc
     1501 gacgttatcc agttcgacaa aaagctggtc gacctcgccc ggcgtggctc cggggtcgag
     1561 ttgcgctttg ccgacggcag ccgcgccttg gccgatggcg tcgtcggcgc cgatggcgtc
     1621 cactccaaag tgcgcgaaat cctgctcggc cccgagcgcc ccatctttac ggggcgcgtg
     1681 gcgcaccgga cggtgtttcc ggccggactg ctcggtgaat ttgccgtcga cacctgcacc
     1741 aaatggtggg ggccggatcg gcacatcgtg gtctacccgg tcaatccccg ccgcgacgaa
     1801 atctatttcg tcaccagcgt gccggacccg cactgggacg tggagtcctg gtcggcgcgc
     1861 ggcgagatgg ccgaggtaca aaatgcgctc gccggtttcc atcccgacgt gcagcgcctg
     1921 ctcgcggcct gcccgcaggt gcataaatgg gccctgttcg agcgcgatcc cttgccacgc
     1981 tggcgcgagg gcccggtcgt gctcctgggc gatgcctgcc accccatgac gccctacatg
     2041 gcgcagggcg ccgccaatgc gctcgaggac gccgcggtgc tgtcgcgctg tctcgccggg
     2101 acggtggatc cggcactggc ttttgcgcgc tacgaagcga cccggcttca acgcaccgcg
     2161 cgcattcagc tgacctcgcg gcagaatacc tggggcaaga ccgcgggcga ccccagctgg
     2221 gtctacggct acaacgcctg ggcgacgccg atcgcggaag cgccgggcct tcccgcctga
     2281 ggcggctcag ccgagcgggc tcaaaagcca ggcggcgatc gccaacaggg ccgccgccgt
     2341 ggcccacatg gcccagcggc tccaacggtc gtggcgtgcc tgggctgccg ccaaccgttc
     2401 gatggtctcg tcgtcgagcc gcaagccagc ctgcgccgtc tcggccagcg cctgggcgac
     2461 cctttccccc tgctcgagca ggcgggggac ctcggccacc accttgccca ggagctcggc
     2521 gccttcgccg gctcccttga ggcggcccgc caccccgtaa ttggtttcca cccactccct
     2581 ggcaatgggc tcggcggcgt tccagatatt gagttccggg tcgagcgagc gcgccacacc
     2641 ctccaccacc accatggtct tctgcagcag caacagctcg gggcgcgtct tcatttcaaa
     2701 cacttccgtg taggcgaaga gctggcccaa cagatcggcg atcgagatct cggaggcggc
     2761 gcgaccgtgg atcggttcgc cgatcgcccg catggcctgg gcgaaaagct caacgggctg
     2821 gtgcggcggc acgtaac
//
LOCUS       DASTMT010000670         2830 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_5828518, whole genome shotgun sequence.
ACCESSION   DASTMT010000670 DASTMT010000000
VERSION     DASTMT010000670.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2830)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2830)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2830
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5828518"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..515
                     /locus_tag="VFR00_15540"
     CDS             <1..515
                     /locus_tag="VFR00_15540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU0160735.1"
                     /translation="FGDERTLVTEPVNIHSVLDHVKRLAETGFTDGVVINVDYDPSLP
                     PVPGNRDKLIQAFLNLVKNAAEAIAELREPGRIVLRTAFRPGMRLSVPGTAARVALPL
                     MIEVEDTGPGVPDQLKAHLFDPFVTTKRKGTGLGLALVAKIIGDHGGVIECESVPKRT
                     VFRVLLPLQD"
     gene            546..1991
                     /gene="ntrC"
                     /locus_tag="VFR00_15545"
     CDS             546..1991
                     /gene="ntrC"
                     /locus_tag="VFR00_15545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HEU0160736.1"
                     /translation="MAGGTVLIADDDSAIRTVLNQAFARAGYLPRATGNAATLWRWVS
                     QGEGDVVITDVVMPDENAFDLIPRIKKLRPDLPIIVMSAQNTLMTALSAAEKGAFEYL
                     PKPFDLSQIVSVVGRALAEPGRRKERSIPEADSEELPLIGRSAAMQEIYRVLARLTRT
                     DLTVMITGESGTGKELVARVLHDYTKRRNGPFVAINMAAIPRELIESELFGHEKGAFT
                     GAQARTEGRFQQADGGTLFLDEIGDMPMEAQTRLLRVLQEGEYTTVGGRTPIKTDVRI
                     IAATHRELKSQIQQGLFREDLYYRLNVVPLRLPPLRERLEDIGDLVRHFLRQTAKQGM
                     GHKAIETAAIERLKHYHWPGNVRELENMVRRLSALHPEDTLTLEIIEAEVADAAPPPN
                     SDSGAAPKELSETIERYLAGHFASYGRELPPPGLYDRMLQHLERPLLMAALAATRGNQ
                     IRAAALLGLNRNTLRKKIRDLDIQVFRTPVS"
     gene            2214..>2830
                     /locus_tag="VFR00_15550"
     CDS             2214..>2830
                     /locus_tag="VFR00_15550"
                     /inference="COORDINATES: protein motif:HMM:NF014808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M17 family peptidase N-terminal
                     domain-containing protein"
                     /protein_id="HEU0160737.1"
                     /translation="MAEPLQVQFAPLSQTPSGTLILLAGEELALGGSARAINERSKGA
                     LLRAAAAAGFTGKAKTVIELLAPAGIDSQRVLLVGAGRPGKELDRLLLGGYAFAQGNA
                     RKGETATLICDPADAGEAGVADFAADLALGGLLRSYAFKKYLTRGKQEEAGEADGGSD
                     GLSKLVILCDRSEAASKAFASRKALADGVFLARDLVNEPANRLGPL"
BASE COUNT          498 a          929 c          941 g          462 t
ORIGIN      
        1 tgtttggcga cgagcgcacg cttgtgaccg agccggtcaa cattcattcg gtgctggatc
       61 acgtcaagcg cctggccgag acggggttca cggacggcgt tgtgatcaat gtcgactatg
      121 atccctccct gccgccggtg cctggcaatc gcgacaagct gattcaggcc tttctcaatc
      181 tggtgaaaaa tgccgccgag gcgatcgccg aactgcgcga gcccggccgc atcgtcttgc
      241 gcacggcctt tcgtccgggc atgcggctct ccgtgcctgg cacggccgcg cgcgtcgccc
      301 tgcccttgat gatcgaggtc gaggataccg gtcccggcgt ccccgaccag ctcaaggcgc
      361 acctgttcga tcccttcgtg accaccaagc gcaagggcac tgggctcggt ctggcactgg
      421 tcgccaagat catcggcgac cacggtgggg tgatcgagtg cgagtcggtg cccaagcgta
      481 cggtgtttcg cgtgctgctg ccgctgcagg actaagaacc ctgacaacag actaggaagt
      541 ggtcaatggc tggtggaact gttctgatag cggacgatga cagcgcgatc cgcacggtgc
      601 tcaatcaggc cttcgcgcgg gccggttatc tgccgcgcgc gacgggcaat gccgccaccc
      661 tctggcgctg ggtttcccag ggggagggcg acgtcgtcat caccgacgtg gtgatgccgg
      721 acgagaatgc cttcgacctc attccgcgca tcaagaagct gcgtccggat ctgccaatca
      781 tcgtcatgag cgcacaaaac acgctgatga cggcgctgtc ggcggcagag aagggcgcct
      841 tcgagtatct gcccaagccg tttgatctga gccagatcgt cagcgtcgtt gggcgggcct
      901 tggccgaacc cgggcgccgc aaggagcgtt cgatccccga ggccgacagc gaggagctcc
      961 ccctcatcgg ccgctccgcg gccatgcagg agatctatcg cgtgctggcg cggctgaccc
     1021 gcaccgacct caccgtcatg atcacgggcg aatccgggac cggcaaggag ctggtggcgc
     1081 gcgtgctgca tgactacacc aagcgccgca atggcccttt cgtcgccatc aacatggcgg
     1141 caatcccacg cgagctcatc gaaagcgagc tgttcggaca tgagaaggga gccttcacgg
     1201 gggcgcaagc gcgcaccgag ggtcgcttcc agcaggcgga cggcggcacc ttgttcctgg
     1261 atgagatcgg cgacatgccg atggaggcgc agacccggtt gctgcgcgtg ctgcaggaag
     1321 gcgagtacac caccgtcggc gggcgaacgc ccatcaaaac cgacgtgcgc attatcgcgg
     1381 cgacgcatcg cgagctcaaa tcccagatcc agcagggact gttccgcgaa gatctctact
     1441 atcgtctcaa cgtggtgccg ttgcggttgc cgcccttgcg ggaacgcttg gaggatatcg
     1501 gcgatctcgt gcgccacttc ctgcgccaga ccgcaaagca aggcatgggg cacaaggcca
     1561 tcgaaacggc cgccatcgag cggctgaagc actaccactg gccgggcaac gtgcgtgagc
     1621 tggagaacat ggtgcgccgg ctgagcgcgc tgcacccgga agacaccttg accttggaaa
     1681 tcatcgaggc ggaggtcgcg gacgcggccc cgccgcccaa cagcgatagc ggggcggcgc
     1741 ccaaggagct ctccgaaacc atcgagcgtt atctcgccgg ccacttcgcc agctacggac
     1801 gcgagttgcc gccgccgggc ctctacgatc gcatgctgca gcatctggag cgtcccttgc
     1861 tgatggccgc gctcgccgcc acgcgcggca accagatccg ggccgcggcg ctgctgggcc
     1921 tcaaccgcaa taccctgcgc aagaaaattc gcgacctcga cattcaagtg ttccgcacgc
     1981 cggtcagttg agagccgcag aaaagcccaa ggcgcggcga tcgccgcctc gacgctcgag
     2041 gggcatcggc gcgcgcccag ggccggcggc tctcgggcgg tgcttgcgct tcagagctga
     2101 gcgtctattg tgaccggcga gtgttgggcc attgcccgca cgcctccccc ttcgacgtgc
     2161 cagcgcgtgg cgggcagcgc gcctcaccgc tttctcacgc acaaggattg ccgatggccg
     2221 aaccgttaca agtccaattc gcgcccttgt cgcaaacccc cagcggcacg ctgattttgt
     2281 tggccggcga ggagctggcg ctggggggga gcgcgcgggc catcaacgaa cgcagcaagg
     2341 gcgcgctctt gcgcgccgcg gccgccgccg gcttcacggg caaggccaaa accgtcatcg
     2401 agctgctcgc cccggcgggt atcgacagcc aacgcgtgct gctggtaggc gccggccgac
     2461 cgggcaagga gctcgatcgg ctgctgctgg gcgggtatgc cttcgcgcaa ggcaatgccc
     2521 gcaaaggcga gacggccacg cttatttgcg atcccgccga tgcgggcgag gcaggcgtgg
     2581 cggactttgc cgccgacctg gcgctgggtg gccttttgcg cagctatgcc ttcaagaagt
     2641 atctgacgcg cggcaaacag gaggaggcag gcgaggccga cggcggcagc gatggtctca
     2701 gcaagctcgt catcctgtgt gaccggtcgg aggccgcgag caaggccttc gccagccgca
     2761 aggcgctggc cgacggcgtg ttcttggcgc gcgatctcgt caatgagccg gccaatcggc
     2821 ttgggcccct
//
LOCUS       DASTMT010000671         1878 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_5831046, whole genome shotgun sequence.
ACCESSION   DASTMT010000671 DASTMT010000000
VERSION     DASTMT010000671.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 1878)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1878)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1878
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5831046"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(43..849)
                     /locus_tag="VFR00_15555"
     CDS             complement(43..849)
                     /locus_tag="VFR00_15555"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU0160738.1"
                     /translation="MLIIDAQVHLWRKGTPSPPHGPTPYLVENAISGMDAAGIDGAIL
                     HPPLSWDPDSNEQAVEAVRTHPRRFAILGYPDLTRPDRQQQIATWKQRPGMLGLRLYF
                     NTPHNRSWPIDGTLDWLWPAAEQADLPIALLAGDWLVRLGEIAVRHPGLRLIVDHMGA
                     LRGAKGDAAFPHMPDLLALAKLPNVAVKLTGGPFYAEDAYPFKSLHRHYRRMYDAFGP
                     HRIFWGTDITKMPCSWRQCVDHMLEIDWLPEADKNLIMGEAICTWLGWKR"
     gene            complement(863..>1878)
                     /locus_tag="VFR00_15560"
     CDS             complement(863..>1878)
                     /locus_tag="VFR00_15560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013418582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HEU0160739.1"
                     /translation="ALKQAQPEFKEPMWQYVNRRVSDWRIITGKARAREYATNIERVE
                     RQYGVDRYLILALWGMESAFGDMVVDRKYMRPVLPALAALAWGEPRRRAYWEQELINA
                     LLIIERGWAQEKEMLGSWAGAMGHTQWMPEVWLTMGVDGNGDGKISPFGAPDDAFAGT
                     AQYLVKRGNYRRGEGWGYEVRLPAGRKPTGHNSIAAWQRLGLVRANGKPFPRPAERAS
                     LWQPVPDGPAFLITQNFRAVRAYNPADSYALATVHLADRIRGEGEFVQQFPGAERIPT
                     LAEVQEMQRLLTARGFDTRGADGRVGPDTVAAVRAFQTKLGIKPADGYAGLKVLAHLR
                     QGA"
BASE COUNT          326 a          589 c          618 g          345 t
ORIGIN      
        1 aaacccgggg caccatcccc ggggcgacgg caggccttcg gttcagcgct tccagccgag
       61 ccaggtgcaa atggcctcgc ccatgatgag gtttttatcg gcctcgggca gccaatcgat
      121 ctcgagcata tggtcgacgc actgccgcca ggagcacggc atcttagtga tgtccgtgcc
      181 ccagaaaatc cggtgcgggc caaaggcgtc atacatccga cggtagtggc gatgcaggct
      241 tttgaacgga taggcatcct ctgcgtagaa cgggccgccc gtgagcttga cggccacgtt
      301 tggcagtttg gcaagcgcta gcaagtccgg catatgcggg aaagcggcgt cgcctttcgc
      361 acccctgagc gcgcccatat gatcgacgat caatctcaag ccgggatgcc ggaccgcgat
      421 ctcgcctaac cgtaccaacc aatcgccggc taacagggcg atcggcagat cggcctgttc
      481 ggccgcgggc cacaaccaat cgagggtccc atcgatgggc caggagcggt tgtgaggcgt
      541 gttgaaataa agacgcaggc ccagcatgcc cgggcgctgc ttccaggtcg cgatctgctg
      601 ctggcgatcg ggtctggtga ggtccgggta gcccagaatg gcaaagcgcc tcggatgtgt
      661 ccgcaccgct tcgacggctt gctcgttgga gtcgggatcc cagctcaagg gaggatggag
      721 tatggcgccg tcaatgccgg ccgcatccat gccgctgatg gcgttttcca ccaaataggg
      781 agtgggcccg tgcggtgggc tcggcgtgcc tttgcgccag agatggactt gcgcatcaat
      841 gataagcatt cgaggccttc cgctacgctc cctgccgcag gtgcgccagc accttcaagc
      901 ctgcataccc atcggcaggt tttatgccaa gtttcgtctg aaacgcacgt accgccgcaa
      961 cggtgtcggg gccgacgcga ccgtcggcgc cgcgcgtgtc gaagcctcgg gccgtcaaaa
     1021 ggcgttgcat ctcctgcact tcggcgagcg tcggtatccg ctcggcgcca gggaactgct
     1081 gcacgaattc tccctccccg cgaatgcggt cggcgagatg cacggtcgcc aaggcgtagc
     1141 tgtccgccgg gttataggcg cgcacggcgc ggaagttctg ggtaatcagg aaggctggcc
     1201 cgtcggggac cggctgccaa aggctggcgc gctcggcggg ccgcgggaac ggttttccgt
     1261 tggcgcgcac aagacccagc ctttgccagg cggcgatgct gttgtggccg gtcggtttgc
     1321 gaccggcggg tagccgcacc tcgtagcccc agccctcgcc gcggcgataa ttgccgcgct
     1381 tcacgagata ctgggccgtg ccggcaaacg catcatcggg cgcgccaaat ggggaaatct
     1441 tgccatcgcc gttgccgtcg acgcccatcg tgagccagac ctcgggcatc cactgggtgt
     1501 gtcccatggc gccggcccag gagcccagca tttccttttc ctgggcccag ccgcgttcga
     1561 tgatgagaag ggcgttgatg agttcctgtt cccagtaggc gcgccggcga ggctcacccc
     1621 aggccaaggc ggcgagcgcg ggaaggacgg gccgcatgta cttgcgatcg acgaccatgt
     1681 cgccgaatgc cgattccatg ccccataggg cgagtatcag atagcgatcg acgccgtact
     1741 gccgctcaac gcgctcgatg ttggtggcat actcgcgggc gcgcgctttg ccggtgatga
     1801 tgcgccagtc ggacacgcgc cgattgacgt actgccacat cggttccttg aattccggtt
     1861 gtgcctgctt gagggcgt
//
LOCUS       DASTMT010000672         3045 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_6487754, whole genome shotgun sequence.
ACCESSION   DASTMT010000672 DASTMT010000000
VERSION     DASTMT010000672.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3045
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6487754"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..436
                     /locus_tag="VFR00_15565"
     CDS             <1..436
                     /locus_tag="VFR00_15565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020053049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEU0160740.1"
                     /translation="MALLGWAYDARLIRALALSLKTREFTYTAVFSGMTTRELLVREH
                     LPYVMPIVFATTMNNMNWSIGLEVTLSVLGFTDINSPTVGGMIYWANQHTALVAGVWW
                     WIAFPVILVVTLFIGLFLLAVSMNEHIDPRSRLRRMGAEGGA"
     gene            433..1473
                     /locus_tag="VFR00_15570"
     CDS             433..1473
                     /locus_tag="VFR00_15570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009764459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0160741.1"
                     /translation="MRPTPAANGETKRPTTEASREPVLRVDDLRAYYRTRHFGVEREV
                     KAVDGVSFEVRDNEIYGLAGESSSGKSTLLKAVAAAMRPPLQVVGGTVRLRFLQAKSL
                     HEASSAELAQLRWRKLSYIMQGSMSALNPVRRVRRSFEDFAARHLRETGAAFDAKVVA
                     HLSHLRLDQGVLDAFPHELSGGMRQRVTIALATICRPDLVIADEPTTALDVVVQKDVL
                     DMIRDLQRQSGSSLLLVTHDLGVHAYLTDRLAIMYGGRLVEEAPTEALFGNPQHPYAK
                     HLIASLPRIGEEAPKAALAGGPPNLAGPPAGCRFHPRCPLAMERCRRETPAMTTIAPN
                     HRVACFAVAGRA"
     gene            1473..2312
                     /locus_tag="VFR00_15575"
     CDS             1473..2312
                     /locus_tag="VFR00_15575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011653789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU0160742.1"
                     /translation="MAPLLELEGVSKSFGGLLGRRRVTAVRDVSFTLSDAKPEVFTLL
                     GQSGSGKTTLARMILGLETPTAGSLRLMGESIAKLRRGARRQRLLGLVQPIFQNPFEA
                     FNPLRHVDHYLLLTAERFGGAGDGPGARAVTEASLRQVGLSLAEVARRYPHELSGGQL
                     QRLAIARALLAKPKLIVADEPVSMVDASLRMAIVNLFKTLRDELAISILYITHDLATA
                     YLVSDRISIMRDGEVVESGAARQVLTHPQHAYSRQLKDAVLVPRFTVGHGDGTSAKSE
                     QRH"
     gene            2330..>3045
                     /locus_tag="VFR00_15580"
     CDS             2330..>3045
                     /locus_tag="VFR00_15580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008968745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HEU0160743.1"
                     /translation="MRHAKVLLDRDFAIGDTDPRLFGAFVEHLGRCVYGGIYEPGHPT
                     ADDKGFRGDVLALIRELGPTIVRYPGGNFVSGYNWEDGVGPVEARPTRLDLAWFSTET
                     NRFGTNEFIDWCRAADVEPMLAVNLGTRGGDAARNLVEYCNHPGGSTYADLRRAHGFP
                     TPHGIKFWCLGNEMDGPWQMEHKTATEYGRVAAEAAKMMRWVDPSLVLAACGSTSRNM
                     PTFGRWDDEVLEHTFELVDFV"
BASE COUNT          486 a         1032 c          970 g          557 t
ORIGIN      
        1 catggcgctc ttgggctggg cctacgatgc gcgtctcatt cgtgccctgg cgctcagcct
       61 caagacccgc gagttcacct acacggcggt gttttccggc atgaccacgc gcgagctgct
      121 ggtgcgcgag cacctgccct acgtgatgcc gatcgtgttc gccaccacca tgaacaacat
      181 gaactggtca attggtctcg aggtgacgct gtcggtgttg ggtttcacgg acatcaattc
      241 cccgaccgtc ggcggcatga tctactgggc caatcagcac actgcgctcg tggccggcgt
      301 gtggtggtgg atcgcctttc ccgtcatttt ggtcgtaacc ctgttcatcg gcctgttcct
      361 gctggccgtg tcgatgaacg aacatatcga tccgcgcagc cggctccggc gcatgggggc
      421 ggagggcggc gcatgaggcc gacgccggcc gctaacgggg agacgaagcg cccgaccacc
      481 gaggcctccc gcgagcccgt gcttagggtc gatgacctca gggcctacta ccgcacgcgg
      541 cattttggcg ttgagcggga ggtcaaggcg gtcgacggcg tctccttcga ggtccgggac
      601 aacgagattt atggtctcgc cggggaatcg agttcgggca aatcgaccct gctcaaggcc
      661 gtcgccgccg ccatgcgccc accactgcag gtagtgggcg gcacggttcg cttgcgattt
      721 ttgcaggcca agtcgctgca cgaggcctcg agcgccgagc tcgcccagct gcgctggcgc
      781 aagctctcct acatcatgca gggctccatg agcgcgctca accccgtgcg tcgcgtgcgt
      841 cgctcattcg aagattttgc cgcgcggcat ttgcgagaga ccggcgccgc cttcgatgcc
      901 aaggtcgttg cccacctttc acacctccgg ttggaccagg gtgtgctcga cgcgtttccg
      961 cacgaacttt ccggtggcat gcgccagcgc gtcaccattg cgctggccac gatttgccga
     1021 cccgacctcg tgatcgcgga cgaacccacc accgcccttg acgttgtggt gcaaaaagat
     1081 gtgctcgaca tgatccgcga cctgcagcgg cagtcgggct catcgctgct gctggtcacc
     1141 catgatctcg gcgtgcacgc ctatctgacc gatcgtctcg ccatcatgta cggcggacga
     1201 ttggtggaag aagcgccgac cgaggccttg ttcggcaacc cgcagcaccc ctacgccaag
     1261 catctgatcg ccagcctgcc gcgcatcggc gaggaggccc ccaaggccgc ccttgcgggc
     1321 ggccccccca atctcgccgg gccgccggcg ggttgccgtt ttcatccgcg ctgtcccctg
     1381 gccatggagc ggtgccggcg cgagacccca gccatgacga cgattgcgcc caaccatcgc
     1441 gtcgcctgct ttgccgttgc agggcgggca tgatggcgcc gctgctcgaa ctcgagggcg
     1501 tgtccaagag cttcggcggt ctgctgggcc gccgccgtgt gacggccgta cgcgatgtga
     1561 gtttcacgct gtccgacgcc aagcccgaag tcttcacgct gctggggcag tcgggcagcg
     1621 gcaagaccac gctcgctcgc atgatcctcg ggctcgagac gccgacggca gggtccctgc
     1681 gcctgatggg cgagagcatc gccaagctgc gccggggcgc gcgccggcaa cgcttgctgg
     1741 gtctcgtgca gccgatcttt caaaatccct tcgaggcctt caaccccctg cgccacgtcg
     1801 atcactattt gctgctgacg gccgagcgct tcggcggcgc cggcgatggc cccggggccc
     1861 gggcggtgac ggaggcgtcg ctgcgccagg ttgggctttc gctcgccgag gttgcccgcc
     1921 gctatcctca cgagctctcc ggcggccaac tgcagcggct ggccattgcg cgtgcgctgt
     1981 tggccaaacc caaactgatc gtcgccgacg agcccgtctc catggtggat gcgtctttgc
     2041 gcatggcgat cgtcaacctg ttcaagacct tgcgcgatga gcttgcaatt tccatcctct
     2101 acattaccca cgatttggcg accgcctatc tggtcagcga ccgcatctca atcatgcggg
     2161 acggggaggt ggtcgaaagc ggcgcggcgc ggcaggtcct cacccacccc caacacgcct
     2221 attcgcgtca actcaaggac gctgtcctgg tgccccgctt caccgtgggg catggcgacg
     2281 gcaccagcgc caagtctgag cagcggcact gagccgggag agccaatcca tgcgccacgc
     2341 caaagtcctt ctcgatcgcg actttgccat cggcgacacc gacccacgat tgttcggggc
     2401 ctttgttgag catttggggc ggtgcgtcta cggcgggatc tacgagccgg gccatcccac
     2461 cgccgacgac aaaggtttcc gcggcgacgt gctggcgctg atcagggaac tggggccgac
     2521 gatagtgcgc tatcccggcg gcaactttgt ctccggctac aactgggagg atggcgtcgg
     2581 ccccgtcgag gcgcgcccga cacggctgga cctggcctgg ttttccaccg agaccaaccg
     2641 cttcggcacc aacgagttca tcgattggtg tcgcgccgcc gacgtcgagc cgatgctcgc
     2701 cgtcaacctc ggcacgcgcg gcggcgatgc ggcgcgcaac ctggtcgaat actgcaacca
     2761 tccggggggg agcacctacg ccgacctgcg ccgtgcgcac gggtttccga caccgcatgg
     2821 cataaagttc tggtgcctcg gcaacgaaat ggatggccct tggcaaatgg agcacaagac
     2881 ggcgaccgaa tacggccgtg tcgccgccga ggccgccaag atgatgcgct gggtcgaccc
     2941 gagcctggtg ctggccgcct gcggctcgac ctcgcgcaac atgccgacct tcggacgctg
     3001 ggacgacgag gtgctcgagc acacctttga gcttgtcgat ttcgt
//
LOCUS       DASTMT010000673         3134 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_6514729, whole genome shotgun sequence.
ACCESSION   DASTMT010000673 DASTMT010000000
VERSION     DASTMT010000673.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3134)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3134
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6514729"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..282
                     /locus_tag="VFR00_15585"
     CDS             <1..282
                     /locus_tag="VFR00_15585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011502181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HEU0160744.1"
                     /translation="TAGEAQLLANAYANSLCLARSHGVRSLAFPAISTGAYGFPEELA
                     ADIALTTVVTALHGDRYFTRVIFCCFGPTAVAQHERARRKVMLARGEEC"
     gene            542..>3134
                     /locus_tag="VFR00_15590"
     CDS             542..>3134
                     /locus_tag="VFR00_15590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0160745.1"
                     /translation="MEGFDVKAALDALLAFIGQNYFLLLIALCLPLLFRRAGVFARVR
                     GAIEKALFENWQLTLLASTGVALSLASAYTTWDGLRNFTRAPLLSLAIAFGIQGVMLI
                     VAWLIGESFAVGMNQRAPGGRRPRLPDAFIGMCLGVTLVGLIFYWVLQHFDAITLSTA
                     AGFHADLAKVGEIGLYFLIGLVLIAIVVFGSRRGGDISTSYAQSLRLIVKNAVLWVMF
                     LSSMAASVFFSFDSHFNAIFPAEARARAAEIRTVGQIGSSIADIGALAQRRQLEEAER
                     LFDTAGWKAYDKQLAHLTRLAQDSQGEIERYFVTQMEERRRAINEQQERIATSHSGQS
                     GLANKKLSLTDELSRLRAERPTLAADYAQHRSELDAKAKEVDAKRIEGMAEDRGVEGS
                     MKQGKGPIYRQRRGELALLNDQYKIKEERTKDAYRRLNGVETRLAQIERELSGIDGEL
                     AKFKGEAETAEQRIKATLAAGSGEEDGKLDPARVLPAFERARAGFRQQPSTERLAALQ
                     QQCSNLVGALSSAEATKGKLRGIDCDPKQAAEAAGRVFALNAGIAVFQARCAGGDKLN
                     GLTSTDALLGFGRSCVQDSGLTGKDSADAGTRLAAIEMNRDDKAHRFVVTWNAFLDGN
                     RLAYLALVLAIGVDSLVFMSGLFGAQALRSPLSDVPSPKARSAEQLQAVIDTALLPHT
                     YENARLMLNAMRPMAAREGFTQCVTLRDDDPHAPDLNRLLNAGATIGAVRHAEANVYE
                     LRSELFEYLSLVAKKSFVADKSHVVLAELERIMAVSLLPNVRENVETVLRYVHPIEDR
                     VGKIGRQERSEFTAEIRLDEVERNDKKVVRNALNAGATLQAVERANNNHYFISRDFYK
                     TLARIRAH"
BASE COUNT          556 a         1019 c          988 g          571 t
ORIGIN      
        1 acggccggcg aagcgcagct attggcgaac gcctatgcca attcgctgtg cctggcgcgg
       61 tcgcacggcg tgcgctcgct tgcctttccc gccatctcca cgggtgccta tggctttcca
      121 gaggagctgg ccgccgatat cgcgctgacg acggtcgtga ctgccttgca tggcgatcgg
      181 tatttcacgc gcgtcatctt ttgctgcttt ggccccaccg ccgtcgccca acatgagcgc
      241 gcgcgccgca aagtgatgct ggcgcgcggc gaagagtgtt aaagaatctt aatgcgcgac
      301 ctgcgctcgc gattcctcgc ctagtcaaat tcgtgacgac gcaacggctt gcacaaatgt
      361 gctgccgccg cgtgcgatcc tggcgtgcgc cagtcgccgt cgacgccacc cgtgcagcgc
      421 attgcatttg tgccagtgga caacaatatc gccgcaaagc ggccaacctg gcgccgcctt
      481 ggcgggcgat gctggaagcg gtgcaacaga tgaagctcgg gtgctagcgg gagacgtgcg
      541 gatggagggc ttcgacgtca aggcggcgct agatgcgctg ctggcgttta ttggtcagaa
      601 ctactttttg cttctgatcg cactatgcct ccccctgctg tttcgccgcg ccggtgtctt
      661 cgctcgcgtc cgcggcgcca tcgagaaggc gctgtttgag aattggcaac tgaccctgct
      721 cgcctccacg ggcgtcgccc tgtcactggc atcggcctac accacttggg acggcttgcg
      781 caatttcacg cgcgcgccgc tgctgtcctt ggcgatcgcc tttggcattc agggggtcat
      841 gttgatcgtg gcatggctga tcggcgaaag cttcgcggtg ggcatgaacc agcgtgcccc
      901 gggcggtcgg cgcccgcggc tacccgacgc cttcatcggc atgtgtttgg gcgtgaccct
      961 cgtgggcctc attttctact gggttcttca gcacttcgac gccatcacgc tcagcactgc
     1021 ggccggtttc cacgccgatc tggccaaggt cggcgagatc ggcctctatt tcctgattgg
     1081 gctggtgctg atcgccattg tcgtgttcgg ctcccgacgc ggcggcgaca tctcgacctc
     1141 ctacgcccag agcttgcgcc tgatcgtcaa gaacgccgtg ctgtgggtga tgtttctctc
     1201 ctccatggcc gcctcggttt tcttctcctt cgatagccat ttcaacgcca tcttccccgc
     1261 cgaggcccgc gcccgcgccg ccgagatccg caccgttggc cagattggct ccagcattgc
     1321 cgacatcggc gccttggcgc agcggcggca gctcgaggaa gccgaacggc tgttcgacac
     1381 ggcgggttgg aaggcctacg acaagcagct tgcgcacttg acccggctgg cccaggactc
     1441 gcaaggcgag atcgaacgct atttcgtgac ccagatggag gagcgacgcc gcgccatcaa
     1501 tgagcagcag gagcgcatcg ccacctccca cagcggccag tcgggacttg ccaacaagaa
     1561 gctatcgctg accgacgaac tctcccgcct gagggccgag cgaccgacgc tggccgccga
     1621 ttatgcccag caccgcagcg aactcgacgc caaggccaag gaggtcgacg ccaagcgcat
     1681 cgaaggcatg gccgaagacc gcggcgtcga aggcagcatg aagcagggca aggggcccat
     1741 ttatcgccag cgccggggcg agctcgcact gctcaacgac cagtacaaaa tcaaggaaga
     1801 gcgcaccaag gatgcctatc ggcgcctcaa tggcgtcgaa acccgcctgg cccagatcga
     1861 gcgggaactg tcgggcatcg acggtgaact ggccaagttc aaaggcgaag ccgagaccgc
     1921 cgagcagcgt atcaaggcaa cgctggcagc cgggtccggg gaagaggacg gcaagctcga
     1981 tccagcgcgc gtgctgcccg cttttgagcg cgcgcgcgcc ggcttccgcc agcagccctc
     2041 aacggaacgt ttggcggctc tgcagcagca gtgcagcaac ctggtcggcg ccctgtcgag
     2101 cgccgaggcc accaaaggca agctccgcgg catcgattgt gatcccaagc aggcggcgga
     2161 agcggccggc cgcgtgtttg ccctcaatgc tggtattgcc gtctttcaag cgcgctgcgc
     2221 cggcggcgac aagctgaatg ggctaaccag cacggacgcc ttgctcgggt ttggccgcag
     2281 ctgcgtgcag gactccggct taaccggcaa ggattcggcc gatgccggca cccggctggc
     2341 ggcgatcgaa atgaaccggg acgacaaggc gcatcggttc gtggtgacct ggaacgcctt
     2401 tctcgatggc aaccggctcg cctacctggc gctcgtgctc gccatcggcg tcgattcctt
     2461 ggtgttcatg tcgggcctgt tcggggcaca ggcgctgcgc tcaccacttt ccgacgtgcc
     2521 aagcccgaag gcgcgcagcg ccgagcagct gcaagcagtg atcgatacgg ccctgctgcc
     2581 gcacacctac gagaatgccc ggctcatgct caatgccatg cgaccgatgg cggcgcgcga
     2641 gggcttcacg cagtgcgtga ccctgcggga tgatgatccg catgctcccg acctcaaccg
     2701 cctgctcaat gccggtgcca ccataggtgc ggtgcgccat gccgaggcca acgtctacga
     2761 gttgcgctcg gagctgtttg aatatctgtc gctggtggcc aagaagtcgt ttgtggccga
     2821 caagtcgcat gtggtgctgg ccgagctcga gcgcatcatg gcggtatcgc tgctccccaa
     2881 cgtgcgcgaa aacgtcgaga ccgtgttgcg ctatgtgcac ccgatcgagg atcgcgtcgg
     2941 caagatcggg cgacaggagc gcagcgaatt tacggccgag atcaggctcg acgaggtgga
     3001 gcgcaatgac aaaaaggtcg tgcgcaatgc cctgaacgcc ggtgccacct tgcaagccgt
     3061 ggagcgcgcc aacaacaacc actatttcat cagtcgcgat ttctacaaga cgctcgcccg
     3121 catccgcgcg cact
//
LOCUS       DASTMT010000674         1547 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_5860755, whole genome shotgun sequence.
ACCESSION   DASTMT010000674 DASTMT010000000
VERSION     DASTMT010000674.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 1547)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1547)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1547
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5860755"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(33..716)
                     /locus_tag="VFR00_15595"
     CDS             complement(33..716)
                     /locus_tag="VFR00_15595"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009031820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEU0160746.1"
                     /translation="MSEQTRWWWIRHAPVPDGGFIYGQRDLACDCSDAEIFGILAREL
                     PRQAVWLTSNLLRTKQTAAAILAAAPNRFAGVEPVEVAALAEQHLGEWQGQERKAFYA
                     ARKVGTHTLWFAPAQERPVGGESFADLVARVVPAIERASAEYSGRDIVAVTHGGTIRA
                     ALALALAVPPQTALGFSIDNCSITRLDYLHPPGISGLWRVGAVNHRPWSRAAAAERLE
                     QNPVGLDKA"
     gene            complement(722..>1547)
                     /locus_tag="VFR00_15600"
     CDS             complement(722..>1547)
                     /locus_tag="VFR00_15600"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003960 - NADPH:quinone reductase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase family protein"
                     /protein_id="HEU0160747.1"
                     /translation="FAPGGEVAGVVAAVGPGVERLKPGDRVIGSCGHGGMADRLVIAE
                     ANCIAMPASMPFDVASALVLTYGTSIHALKDRAGIKSGETLLVLGAAGGVGLSAVELG
                     KAFGARVIAAASSQDKVALAREHGADGGVVYPPGPFDKAGTKALSELFKAACGANGAD
                     VIYDPVGGDYTEAALRAIAWEGRLLVVGFPAGIARLPLNLTLLKSCQIVGVFWGGFAR
                     REPKANAANIAELMQLYAKGSIKPRISERYPLVRAGEAITRLAQRKAMGKIVVTMD"
BASE COUNT          278 a          530 c          500 g          239 t
ORIGIN      
        1 acgggcctga cgcggccgaa ggccgtcggc gctcaagcct tgtcgaggcc gacagggttt
       61 tgctcgagtc gttcggcggc ggcggcgcgt gaccagggtc ggtgattgac cgcacccacc
      121 ctccacaagc cggagatgcc cggcgggtgc aaatagtcga ggcgcgtgat cgaacagttg
      181 tcgatcgaaa agccgagcgc cgtctgcggc ggcacggcca acgccagcgc cagggcggct
      241 cgaatggtgc cgccgtgcgt taccgcaacg atgtcgcggc cactatactc ggcgctggca
      301 cgctcgattg ccggcacgac gcgggccacg agatcggcaa agctttcccc gccaaccggc
      361 cgctcctgcg cgggcgcaaa ccacaaggta tgggtgccga ccttgcgcgc ggcataaaag
      421 gccttgcgct cctggccctg ccattcgccc aaatgctgtt cggccaatgc cgccacctcg
      481 accggctcta cgccggcaaa gcggttgggt gccgcggcca ggatcgccgc agcggtttgt
      541 ttggtgcgca agagattgct ggtcagccag accgcctgac gcggcaactc gcgcgcaaga
      601 atgccgaaga tctccgcatc gctgcagtca caggccaggt cgcgttggcc atagataaaa
      661 ccgccatcgg gcacgggcgc atgccggatc caccaccatc gtgtctgctc cgacatgacg
      721 ctcaatccat tgtcacgacg atcttgccca tggccttgcg ttgcgccagc cgggtaatgg
      781 cctcgccggc gcgcaccagg ggatagcgct ccgaaatgcg cggtttgatc gagcctttgg
      841 cataaagctg catcaactcg gcaatattgg cggcgttcgc cttgggctcg cgccgcgcga
      901 agccgcccca gaacacgcca acgatttggc aggacttcaa gagcgttaga ttgaggggca
      961 ggcgggcgat gccggcgggg aaaccgacca ccagcaagcg tccctcccag gcgatggcgc
     1021 gcagcgccgc ctcggtgtag tcgccaccca ccggatcata gatgacgtcg gcaccgtttg
     1081 ctccacatgc tgccttaaac agctccgaca aggccttggt gcccgccttg tcgaatggcc
     1141 cgggcggata gaccacaccg ccgtcggcgc cgtgctcgcg ggcaagggcc accttgtcct
     1201 gtgaagaggc cgcggcaatg acgcgcgcgc cgaacgcttt gccgagctca accgctgaca
     1261 ggcccacgcc gccggcggca ccaagcacaa gcagcgtctc gccggacttg atgccggccc
     1321 gatccttgag ggcatggatg gacgtgccgt aggtcaagac cagggcgctg gcgacgtcga
     1381 agggcatgct ggccggcatg gcgatgcaat tggcttccgc gatcacgagc ctgtcggcca
     1441 tgccgccgtg accacacgag ccgatcacgc ggtcgccggg cttcagccgc tcgacccccg
     1501 gtccaacggc ggcgaccacg cctgctacct cgccacccgg agcgaat
//
LOCUS       DASTMT010000675         4301 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_6523599, whole genome shotgun sequence.
ACCESSION   DASTMT010000675 DASTMT010000000
VERSION     DASTMT010000675.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4301
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6523599"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..162)
                     /locus_tag="VFR00_15605"
     CDS             complement(<1..162)
                     /locus_tag="VFR00_15605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006643365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEU0160748.1"
                     /translation="MDSRNIAIIAHVDHGKTTLVDCLLKQSGTFRANQHEAQEERLMD
                     SMDLEKEKGI"
     gene            535..1053
                     /locus_tag="VFR00_15610"
     CDS             535..1053
                     /locus_tag="VFR00_15610"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HEU0160749.1"
                     /translation="MFERNRVDNAPEISAVPVEITGLDGTLIKGKLLVPVPKSVAEAL
                     NGAAAFIEFEPYGGEKRYLAKAQLAAVKPLGVPRLPSLQGRLRQSEEFDPHAVLGVPC
                     GASREEIREAYVTLAKAYHPDRYANVTLPPEVSEYLAAMARRINLAHAALEGPEKRKA
                     LRQEPIFTSPGR"
     gene            complement(1088..1921)
                     /locus_tag="VFR00_15615"
     CDS             complement(1088..1921)
                     /locus_tag="VFR00_15615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0160750.1"
                     /translation="MTRSTLKATMRRVYALIGSVLAICLLAKFVDHIPALRGSGLEAL
                     LKDAYEFLRDMSLLIATGGVAYITNIYQKRSSFVDALKQEWRDIIAAKSALFSYTQLE
                     RPTLEHYLATFCTLSETIDNMRSVYQNVGETDDLVGLYPYAPLHDMRRALQTLDPRKT
                     PQAADSETRALVRDAILQSFYALRETFLEELDPEAPDHPLLISGGRRAKQSGSTGAAR
                     RRQERQRSRQDRLTSPDPRIDQLLADLMQKEEQTAKPWRQPPAGAARAAALAPANGRA
                     S"
     gene            complement(1947..2588)
                     /locus_tag="VFR00_15620"
     CDS             complement(1947..2588)
                     /locus_tag="VFR00_15620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009029901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0160751.1"
                     /translation="MPLQNRVTPAGDIITTPQRGLMMGNRGGAFHLPDRTLGSRRWAS
                     RQWIACVLQFKGRHRLAMMQPNRYTELFFLDEATALAAGHRPCFECRRRDAERFAMLW
                     AHTRNWPAPARAPEMDLALHAERIGRHGQKLTFTAQLGALPVGSFVRLPDSAEQVDYL
                     VLAARLLAWSPAGYANPLPLNRRRQVEVLTPRSIVAVLSAGYRPMLHPSAGEA"
     gene            2630..3412
                     /locus_tag="VFR00_15625"
     CDS             2630..3412
                     /locus_tag="VFR00_15625"
                     /inference="COORDINATES: protein motif:HMM:NF004795.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEU0160752.1"
                     /translation="MSDSSVQVKLEERPDGASIATVSIDNQRRLNCLSTPLIVELTGV
                     IRRLANAKTLRALVLTGAGEKAFIGGADLNELGGLCADSARLFITRLHEACLALRDCP
                     VPVIARINGFCLGAGLEIAASCDLRAASEHAQFGMPEVQMGLPSVIEAALLPGLIGWG
                     RTREMLLTGTLCSATAAAQMGFLQRLAPADQLDAAIAEWLGAICRAGAQAVRAQKALI
                     NRWQRVSVDEGIYAGIDALAEAYGTGEPQAAIKTFFANKHKA"
     gene            complement(3449..>4301)
                     /locus_tag="VFR00_15630"
     CDS             complement(3449..>4301)
                     /locus_tag="VFR00_15630"
                     /inference="COORDINATES: protein motif:HMM:NF019151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HNH endonuclease family protein"
                     /protein_id="HEU0160753.1"
                     /translation="PADAAARIYEELRQVRVQQSVHAPAIARSLTYLNRLRFSDWIAP
                     ALLWRLQKGDDAGELAWFLAALDRLALGIRLLGLGAHKRATWFAAVTTAIRSGTDLKV
                     ANGVLSFSRQDVRTMQYNLRDLHARDASTAKHLLLRLTDHMSGEPQSLAFPDDMSVEH
                     VLPRKSSPNSQWRGWFADPTEREIYTESLGNLVLVTKSQNDRAGNLDFARKLEVYFKT
                     AGAPLPRLNEALRGLTEWKAPQIKSREVELLRLVESMWKFGLKLSSEEAAEASPGVAT
                     RRRRASG"
BASE COUNT          748 a         1371 c         1441 g          741 t
ORIGIN      
        1 gatgcctttt tccttttcca ggtccatcga atccatcaag cgttcctcct gcgcctcgtg
       61 ctgattggcc cggaacgttc cggactgctt gaggagacaa tccaccagcg tggtcttgcc
      121 atggtccacg tgagcgatga tcgcgatatt gcgactgtcc attgcgggtc cttcaggaac
      181 gggcctggcg cagaggcccg gcgtgcggct cgcgattttg cagcgcacca actttgcggc
      241 cgcgcgtagc atattccgcg ccgcgccgcc agcgcgccgc cggggcgtgc ggcccgcggc
      301 gcgccctatt tggctcaact ggccaagact ttggggggag ccctgccagg cccgccggag
      361 gcgggttgcc gccgagtagc aagcggggcc caatggccgc gccagaggtc gggtgccgct
      421 cgccggcccg tcgacggtgc gcgagcggat tttggcgccg caggcccaat tcttgctgcc
      481 aaggcgcatg cggggcggcc ggcgtgccgt catgacacag gtcgagagta gcccatgttt
      541 gagcgcaata gggtcgataa cgcgcccgag atcagcgccg tgccggtgga aattaccggc
      601 ctcgacggca ctctcatcaa aggcaagctg ctggtgccgg ttcccaagag cgtcgccgag
      661 gcgctcaacg gcgctgccgc tttcatcgag ttcgagccct acggaggcga gaagcgctac
      721 ctggcgaagg cgcaactcgc cgccgtcaaa ccgctcggcg ttccgaggct gcccagcttg
      781 cagggccggt tgcgccagag tgaagaattt gatccgcatg ccgtgctcgg ggtaccctgc
      841 ggtgccagcc gggaggagat ccgcgaggcc tatgtgacgc tcgccaaggc ctatcatccc
      901 gatcgttacg ccaacgtcac gctgccgccc gaggtgagcg aatacttggc cgccatggca
      961 cggcgtatca atcttgcgca cgccgccctg gaagggcccg aaaagcgcaa ggcgctgcgc
     1021 caggagccga ttttcacgtc ccctgggcgc taacgaccac ctttcgtttg gcgccgctgc
     1081 tgggggctca ggaggcgcgt ccgttcgccg gggccagcgc ggccgcgcgc gccgcgccag
     1141 ccggcggctg ccgccagggt ttggccgtct gctcttcttt ttgcatgaga tcggccaaga
     1201 gttggtcgat gcgcggatcc ggcgacgtca gacgatcctg gcgcgagcgt tggcgctctt
     1261 gccgccgccg cgccgcgccc gtggagcctg actgtttggc gcgccgtccg ccggaaataa
     1321 ggagtggatg gtcgggagcc tcgggatcga gctcctccag aaacgtctcc cgcagggcat
     1381 agaacgattg caggatggcg tcgcgcacca gggcgcgcgt ttccgaatcc gcggcctggg
     1441 gggtcttgcg cggatcgagg gtctgcagtg cccggcgcat gtcgtgcaac ggcgcatagg
     1501 gatagaggcc gaccaggtcg tcggtctcgc cgacgttttg ataaaccgag cgcatgttgt
     1561 cgatcgtttc cgacagcgtg cagaaggttg caagatagtg ctccagcgtc ggccgctcga
     1621 gctgggtata ggagaacagc gccgacttgg ccgcgataat atcgcgccac tcctgcttca
     1681 gcgcgtccac gaagctcgac cgcttctgat aaatgttggt gatataagca acgccgcccg
     1741 tggcgatcaa aagcgacatg tcacgcagaa attcgtaggc atccttcaac agcgcctcga
     1801 gcccagagcc cctcaaggcg ggaatgtggt cgacgaattt ggctaaaagg caaatcgcca
     1861 ggaccgagcc aatgagcgcg taaacacgcc gcatggtggc tttgagtgtg gagcgggtca
     1921 tacgtgtcgt tccccaaacg cgctgttcag gcctcgccgg ccgaggggtg cagcatgggg
     1981 cgatatcccg ctgaaagtac ggcaacgatg gagcgcggcg tcaacacctc gacctgccgg
     2041 cgccgattga gcggcagcgg attggcgtag ccggccggcg accacgccaa aagacgtgct
     2101 gccagcacga gataatccac ctgttcggcg ctgtcgggaa gccgaacgaa gctgccgacc
     2161 ggtagcgcgc cgagttgggc cgtaaaggtc agcttctgtc cgtgccggcc gatccgctca
     2221 gcgtgcagcg ccaagtccat ttcgggagcg cgcgcgggcg ccggccagtt acgcgtgtgc
     2281 gcccatagca tggcgaatcg ctccgcatca cggcggcggc actcaaagca gggccgatgt
     2341 cccgccgcca gtgcggtcgc ctcatcgagg aagaacagct cggtgtagcg attgggttgc
     2401 atcatggcca ggcggtgtcg gcccttgaat tggagcacgc aggcgatcca ttgccggctg
     2461 gcccagcgcc ggctgccgag cgtgcggtcg ggcagatgaa aggcgcctcc gcgattgccc
     2521 atcatcaggc cgcgctgcgg cgtggtgata atgtcgcccg cgggcgtgac acggttctgg
     2581 agcggcatgc ggtgatctgg cagaattcga agcaacaggg agacagagaa tgtcggatag
     2641 cagcgtgcaa gtgaagctgg aagaacgccc cgacggtgcc tcaattgcaa ccgtcagcat
     2701 cgacaatcag cgccggctga actgtctttc gacgccgctc atcgtcgagc ttaccggggt
     2761 catacggcgc ctggcgaacg ccaagacatt gcgcgcgctc gtgctcacag gcgcggggga
     2821 gaaggccttc attggcggtg ccgatctcaa cgagctcggc ggtttgtgcg ccgacagtgc
     2881 gcgccttttc atcacgcgcc tgcacgaagc ctgcctggcg cttcgcgact gcccagtgcc
     2941 ggtgatcgcg cgcatcaacg gcttttgcct cggtgccggc ctcgagattg ccgcgagctg
     3001 cgacttgcgc gctgcgagcg agcacgcgca attcggtatg cccgaggtgc aaatggggct
     3061 tccgtccgtg atcgaagcgg cgctgttgcc cggcctcatc ggttgggggc gcacccgcga
     3121 gatgctgctg acgggcacgc tgtgttcggc cacagccgcc gcccagatgg gcttcctgca
     3181 gcggttggcg cctgccgatc agctcgacgc cgcgatcgcc gaatggcttg gcgccatctg
     3241 tcgcgccggc gcacaggccg tccgcgccca gaaggcattg ataaaccgtt ggcagcgcgt
     3301 ctccgtcgat gaggggatct acgccggcat cgatgccctc gcggaggcct atggcacggg
     3361 tgagccgcag gcggccatca agaccttctt tgccaacaag cacaaagcct aggccagggc
     3421 gccgctgaaa gcgcggcatg ccggcaaatc agccggacgc ccgccgccga cgcgtcgcca
     3481 cgccgggcga agcctctgcg gcctcttcgc tcgatagctt cagcccgaat ttccacatgc
     3541 tttcgaccag ccgcagcagc tcgacctcgc gcgacttgat ttgcggggcc ttccactccg
     3601 tcaagccacg cagcgcttcg ttgagcctcg gcagcggcgc cccggcggtc ttgaaataaa
     3661 cctcgagctt gcgcgcaaag tcgagattgc cggcgcgatc gttctgggac ttggtgacca
     3721 gcaccaggtt ccccaacgac tcggtgtaga tttcgcgctc ggtgggatcg gcaaaccagc
     3781 cacgccattg actgttcggg ctcgattttc tgggcagcac gtgctccacg ctcatgtcgt
     3841 cgggaaaagc aagcgattgg ggctcgcccg acatgtgatc ggtgagccgc agcagcaggt
     3901 gcttggcggt ggaggcgtca cgcgcgtgca ggtcgcgcaa gttgtactgc atcgtgcgca
     3961 cgtcctggcg tgaaaagctg agcacgccgt tggcaacctt gagatcggtg ccgctgcgga
     4021 tagccgtggt caccgcggcg aaccaggttg cccgcttgtg ggcgccgagc ccgaggaggc
     4081 gaatgccaag cgccaggcgg tcgagcgcgg ccagaaacca ggcgagctca ccggcatcgt
     4141 cgcccttctg caaccgccac agcagggccg gcgctatcca atcggaaaat cgcaggcgat
     4201 taagataggt aagcgagcgc gcgatcgccg gagcatggac actttgctga acgcgaacct
     4261 ggcgcaattc ctcgtagatg cgcgcggcgg catccgcagg c
//
LOCUS       DASTMT010000676         1646 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_6541632, whole genome shotgun sequence.
ACCESSION   DASTMT010000676 DASTMT010000000
VERSION     DASTMT010000676.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 1646)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1646)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1646
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6541632"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1191)
                     /gene="glp"
                     /locus_tag="VFR00_15635"
     CDS             complement(<1..1191)
                     /gene="glp"
                     /locus_tag="VFR00_15635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002711633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HEU0160754.1"
                     /translation="MALMPVAEALQRVLTNASALSAEMVPLSDALGRVLTGDVIALRT
                     QPPAAVSAMDGYAVRGADVAAAPIALKVIGEVAAGHPFDGQVGPHQAARIFTGGIMPT
                     GSDTVVIQEHTTRAGDSVTIEKPTSKKRNVRLQGIDFASEQILLRRGRRLTDRDLMLA
                     AAMNHSRLSVHRRPKVAVLGTGDELVPPGGKPGPGEIVYSNGFALSALARSEGADVHD
                     LGIARDRVEDIVAAVRRAREWRADVLVTSGGASVGEHDLVQRALTAEGLDLSFWRVAL
                     RPGRPMMHGRLGDMQVLGVPGNPVSSFVCAFLFLVPLIRRLAGRPDVEQAPEAARLGC
                     DLPANDERQDYLRATLAAGANGLIATPLPNQDSSLMAPLARADCLLIRAPHAPAAATG
                     SDCVI"
     gene            complement(1195..>1646)
                     /gene="moaC"
                     /locus_tag="VFR00_15640"
     CDS             complement(1195..>1646)
                     /gene="moaC"
                     /locus_tag="VFR00_15640"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011503235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HEU0160755.1"
                     /translation="GEARMVDVSEKGTTARIAVAEGRVLMTKETLDLVIEGNAMKGDV
                     LGAARLAGIMAAKRTHGLIPLCHPLPLSKIEIEINPEHSLPGFLVQATVKVTAKTGVE
                     MEALTAVSIGCLTIYDMVKAVERGVHIEGIRLLRKSGGKSGEWHAES"
BASE COUNT          298 a          530 c          537 g          281 t
ORIGIN      
        1 gatgacgcag tctgaaccgg tggcggccgc tggcgcgtgt ggcgcccgaa tcagcaggca
       61 gtcggccctg gccaaggggg ccatcaggga actgtcctgg ttcggcagtg gcgtggcaat
      121 caggccgttg gcgccggccg caagggtggc ccgcagatag tcctggcgct cgtcattggc
      181 cggcagatcg cagccgaggc gggcggcttc gggggcctgc tcaacgtcgg gccgccccgc
      241 caagcgccgg ataaggggca caaggaacag gaaggcgcaa acaaaggacg atacggggtt
      301 gccgggcacg cccagaacct gcatgtcgcc cagccggcca tgcatcatgg ggcgcccggg
      361 gcgcaacgcc acgcgccaaa acgaaagatc gagaccctcg gccgtcagag cgcgctgcac
      421 caggtcgtgt tccccgacag aagcgccacc cgaggtgacc aggacatcgg cgcgccactc
      481 gcgagcccgc ctcacagcag caacgatgtc ctcaacgcgg tcgcgggcaa tgccgagatc
      541 atgcacgtcc gcgccctcgc tgcgggccaa tgcgctcagg gcaaaaccgt tggaataaac
      601 gatctcgccc gggcccggct tgccgccggg agggacgagc tcgtcaccgg tcccgagcac
      661 ggccaccttt ggtcgccggt ggacgctgag gcgggaatgg ttcatggcgg cggcgagcat
      721 caggtcgcgg tccgtgagcc ggcgccctct gcgaagcaga atctgttcgc tggcaaagtc
      781 gatgccttgc aggcgtacgt tccgcttttt cgatgtcggc ttctcgattg tgacggaatc
      841 gccggcacgc gtggtgtgtt cctgaatcac cacggtgtcg ctgcctgttg gcatgatacc
      901 gccggtaaaa atgcgcgcgg cctgatgggg accgacttgt ccgtcgaatg gatgacctgc
      961 tgcaacttca cctataactt taagtgcaat gggcgctgca gccacgtcgg cgccacgcac
     1021 ggcgtagcca tccatcgcag agacggccgc agggggctga gtgcgcagcg cgatgacgtc
     1081 gccggtgagg acgcggccga gcgcatcgct aagcggcacc atttccgctg aaagagcgct
     1141 cgcgttggtc agcacgcgtt gcagcgcttc ggcaaccggc attagggcca ttgactagga
     1201 ctccgcgtgc cactcgcccg atttgccgcc gcttttgcgc agcaggcgaa tgccttcgat
     1261 gtgcacgccg cgctcgaccg ccttgaccat gtcgtagatc gtcaagcagc cgatcgaaac
     1321 ggctgtcaat gcttccatct ccacgcccgt tttggccgtg actttcaccg tcgcctgcac
     1381 cagaaagccg ggcagcgaat gctccggatt gatctcgatt tcgatctttg aaagcgggag
     1441 cggatggcag agcggaataa gaccatgggt gcgctttgct gccatgatgc cggcaagccg
     1501 agccgcgccc agtacatcgc ctttcatcgc attgccctcg atgacgagat cgagggtttc
     1561 tttggtcatc agcacgcgcc cctcggcgac cgcaatgcgc gcagtcgtcc ctttttcaga
     1621 gacatcgacc atgcgggcct cgccgc
//
LOCUS       DASTMT010000677         5842 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U3799
            k141_5903306, whole genome shotgun sequence.
ACCESSION   DASTMT010000677 DASTMT010000000
VERSION     DASTMT010000677.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970067
            Sequence Read Archive: SRR11613357
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 5842)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5842)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 23:08:18
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,132
            CDSs (total)                      :: 3,117
            Genes (coding)                    :: 3,104
            CDSs (with protein)               :: 3,104
            Genes (RNA)                       :: 15
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 9
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5842
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5903306"
                     /isolate="SMAG_U3799"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="26.6539 N 80.6288 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..771)
                     /locus_tag="VFR00_15645"
     CDS             complement(<1..771)
                     /locus_tag="VFR00_15645"
                     /inference="COORDINATES: protein motif:HMM:NF045340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HEU0160756.1"
                     /translation="MPISGRLRLAVLLAVASVLGACTLPWQQLKLPGPVPPDAGPAPI
                     ARANHSPGSPLTPGAPEVAGPQISPGTGQLTGTPQPRPVVGAGAANSKDGVTLNFVGA
                     SIAEAARSILGDILGVNYTVSDKLKGSITIQTVRPIPKEALLGVFETMLSGEGAAISV
                     DNGIYRILPSNDAVSAAAILARGARRLPGVSAQVVALRYVAAAEMERIVKSMAPQATI
                     LRTDPARNLLVVAGTRGDLDSIMDAVSTFDVDWMKGMSF"
     gene            1325..3655
                     /locus_tag="VFR00_15650"
     CDS             1325..3655
                     /locus_tag="VFR00_15650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007800481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HEU0160757.1"
                     /translation="MYLPKDAPQNRIREALKARANRLEIVRALAAGQVTRRELFKWGL
                     FTSSGLLAWKHGLNVYTPSAYGQVPTGTPRSPLFGARKFTQPLHRLEVARPLTLTRTA
                     GGEAAFPSGSNEPNAKRLSYHQDFNTWKTANSGQPTSANPFCNPVTKRGPMEGRPPGE
                     LFAHQRWDEFFPKVGYVLSLGEIDAGYGLHPAMPSQQPNSIWTYGVGRNARGSSPPLL
                     IKARYGEPLLMRVYNRLKDPELPEYKGQDLNRGFGRAETQLHFHNAHNGAESDGASNV
                     HHFPGTFYDYRWSTTLARRDKINTGASIKKASGPDGNGGLVYVAGDFRELQGSMWAHD
                     HRFFFTAENVYKGNFMAVNFYSGPDRGNEKLTDGINLKLPSGSLLDWGNVDFDVNLVI
                     HDYATAADGQCFFDIFTTDGFLGDVLCVNMQFGPFLEVLPRKYRFRLLNACMSRFVKL
                     ALSWNWTAVPFQFIANDGNLVVNPINLLELDEQGSAERYDIVVDFSKFRIGDRVYLVN
                     LLQQRDGRKPDAALSLREAMAGVPDKDPAVGPLLEFRIVSAVSSVDVPGYVHKAATLD
                     KSLVPAALTQQIPIVAPVRTRVVEFGRSGDGNARGPDGQCTPDCPEIATSFPWTIKIN
                     GEDAHSFNANRISLLIPKPGEIEHWTYINGGGGWDHPIHLHFEEGITMNRGGASFPAT
                     ENLVRKDVWRLRPSGRVQFQVQFGEYGGSYVNHCHNTVHEDFAMLMRIQLLGKAGTPQ
                     AAVTLTPNPSRDGVTFTSPEILREGDPRTTQTASSG"
     gene            3660..5060
                     /locus_tag="VFR00_15655"
     CDS             3660..5060
                     /locus_tag="VFR00_15655"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEU0160758.1"
                     /translation="MLFALRPLAAALLAAATLSPAAAGEASRWGKGYVPNHPVEAQDG
                     QSFNFYDDLIQDRIAVVSFIYTSCSEICPVATARLAQLQEKLGEHMGRDVFFYSITVD
                     PETDTPARMQTYAKTFGAGPGWLFLTGKPEHINEIRYRLGERSRFLGEHRNDLLLSNG
                     FAGQWQRDSAMGDLDRLILTLQSLNPAAFGAAPLAGTPHQQLEAIELTGPPGETLYLK
                     ACAGCHSIGEGNRVGPDLAGVTQRRQPEWLINFIANPAKVRASKDPIALELAARFPTV
                     RMPAIGVSAREAADLVAYIEARQARSQRRIALAPLLALTTAEGKRLAAPEIEGRALAV
                     TFGFTHCPDVCPTTLMDWSNVLASLGPDGDRLKVLFVSVDPERDTPAVLKSYMASFDP
                     RITALTGSAGELAAVAAAFGAHYAKVPGQHGSYTLDHTLKTFILDAEAQLAGASDHAT
                     DPQQRRELLKKLAGRE"
     gene            complement(5091..5663)
                     /locus_tag="VFR00_15660"
     CDS             complement(5091..5663)
                     /locus_tag="VFR00_15660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU0160759.1"
                     /translation="MARAQKGGSARPLLLTALLGGVAVLSLVNWHLYSTAVDISPLSP
                     QTRQVDAKEARDHELATPLDDKPQIVFEETVQRPLFHADRKPIERTKEVVEGPAPAAL
                     NTGMQLVGIVKSAGAPGRALIRFAGEPIGKWVPEGETVNGWHLASVKTSSVVLEGNGQ
                     THELQLPLPPRSAGGEGGERPNEPPKRKTR"
BASE COUNT         1004 a         2044 c         1844 g          950 t
ORIGIN      
        1 aaacgacatg cccttcatcc agtcgacgtc gaaggtgctg accgcgtcca tgatggaatc
       61 gaggtcgccg cgcgtgccgg ccaccaccaa caggttgcgc gccggatccg tgcgcaggat
      121 ggtggcctgc ggcgccatcg acttgacgat gcgctccatc tccgcggccg ccacgtagcg
      181 cagcgccacc acctgtgcgc tgacgccggg cagccgccgg gccccgcgcg ccaggatggc
      241 ggcggccgat acggcatcgt tggagggcag gatgcggtag atgccgttgt cgaccgagat
      301 cgccgcgcct tccccgctca acatggtctc gaacacgccg agcagggcct ccttcgggat
      361 cggacgcacc gtctggatgg taatggagcc cttgagcttg tcgctgaccg tgtaattgac
      421 gcccaaaatg tcgccgagga tggagcgagc ggcttcggcg atgctggcac cgacgaaatt
      481 gagcgtgacg ccatccttgc tgttggccgc gccggcgccg acgacggggc gtggctgcgg
      541 ggtccccgtc aattggcccg tgccgggcga aatctgtggc ccggccacct ccggcgcgcc
      601 cggcgtcagg ggggacccgg gcgagtggtt ggcgcgggcg atgggggcgg ggccggcgtc
      661 aggcggcacc gggccaggca gcttgagctg ttgccagggc aacgtacacg cgcccagcac
      721 gctcgcaacc gccagcaaga ccgctaaccg caatctgcct gaaatcggca cgatccccct
      781 cgaagtcagc atacgcaaga ccgttgcgca tacgtccgtt caagcgcaca cccgtgacag
      841 attcgaattt cccgaccaaa cacctcggcg agtgatccac ggtcgaagcc gccgcggttg
      901 ggcacccaaa aacgaggcga gcttattcag ttttgccggg cccgtcgatg ctgattcggg
      961 cctaccactt tgtctccata gcactttcgc cgggtagatg ccgaattgtt acgccgcctt
     1021 tttcaggccc gcaaatagcg ctgttcatga ccagactgtt ggtccctgcg tgtcagacaa
     1081 aggtccgctg tgcgggcgcg ggagacgccg taaggaggcc aggctcgtcg cggcgccggg
     1141 ctaatccccg gaggtgacga cgccaggagc gcgagggccc gcacagggcc gccggcgccg
     1201 agactgaagg tgaggtcccc ggccgccgcg cggccagggt gctcggccta cccgacggca
     1261 ggctggggtc tcaacattcc ttccatcagc ggggttgcac gcgctgtggc gcaaggggtt
     1321 tgctatgtat ttgccgaagg atgcaccgca aaaccgcatt cgagaagccc tcaaggcccg
     1381 cgccaaccgc ctggaaatcg tgcgcgccct ggccgcgggg caagtgacgc ggcgcgagct
     1441 cttcaaatgg ggccttttca cctcgagcgg cctcttggcg tggaagcatg gcctcaacgt
     1501 ctataccccg agcgcctacg gccaggtgcc caccggcacc cccagaagcc cgctgtttgg
     1561 ggcgcgaaaa ttcacccaac cgctgcaccg gctggaggtc gcgcgcccgc tcaccctgac
     1621 gcgcaccgcc ggcggtgagg ccgcctttcc ctcgggctcc aacgagccca acgccaagcg
     1681 gctgtcctac catcaggatt tcaatacttg gaagacggcc aattccggcc agccgaccag
     1741 cgccaacccc ttttgcaatc ccgtcaccaa gcgtggcccg atggagggac gaccgcccgg
     1801 cgagctcttt gcgcatcagc gctgggacga gttcttcccc aaggtgggct atgtcctctc
     1861 gctgggcgaa atcgatgctg gctatggcct gcatcccgcc atgccctccc agcagccaaa
     1921 cagcatctgg acctacggcg tcggccggaa cgcgcgcgga tcctcaccgc cgctgctgat
     1981 caaggcgcgc tacggcgagc cactgttgat gcgcgtctac aatcgcctca aggatcccga
     2041 gctgcccgag tacaaggggc aggacctgaa tcggggcttc gggcgcgccg aaacgcagct
     2101 gcatttccat aacgcgcata acggcgcgga aagcgacggc gcctccaacg tgcatcattt
     2161 ccccggcacc ttctacgact accgctggag cacgacgctg gcccgccgcg acaagatcaa
     2221 caccggcgcc agcatcaaga aggcctccgg gcccgacggc aacggtggcc tcgtttatgt
     2281 ggccggcgac ttccgcgagc tgcagggcag catgtgggcg cacgaccacc gtttcttctt
     2341 caccgccgag aatgtctaca agggcaattt catggcggtg aatttttata gcggacccga
     2401 ccgcggcaac gagaagctga cggatggcat caatctcaaa ctgccgagcg gcagcctgct
     2461 cgattggggc aacgtcgact tcgacgtgaa tctggtcatt cacgactacg ccaccgccgc
     2521 cgacggccag tgcttcttcg acatcttcac gaccgacggc ttcctcggcg atgtcctgtg
     2581 cgtcaacatg caattcggcc ccttcctgga ggtgttgccg cgcaagtatc gcttccgcct
     2641 gctcaacgcc tgcatgtcgc gcttcgtcaa gctggcgctg tcctggaact ggacggccgt
     2701 tcccttccag ttcatcgcca acgacggcaa cctggtggtc aatcccatca atctcctgga
     2761 attggacgaa cagggcagtg ccgagcgcta cgacatcgtc gtcgactttt ccaagttccg
     2821 catcggcgat cgcgtctatc tcgtcaacct gttgcagcag cgggatgggc gcaaacccga
     2881 cgcagcgctc agcctgcgcg aggccatggc tggcgttccc gacaaggacc cggccgtcgg
     2941 tccgctcttg gagttccgga tcgtgagcgc cgtcagcagc gtcgacgtgc cgggctacgt
     3001 gcacaaggcc gccacactcg acaagagcct ggtgccggcc gcgctgaccc agcagatccc
     3061 gatcgtggcc ccggtcagga cgcgggtggt ggagttcggg cgctccggcg acggcaacgc
     3121 gcgcggcccc gacggccagt gcacgcccga ttgtcccgag atcgccacca gctttccctg
     3181 gaccatcaaa atcaacggcg aggatgcgca ttccttcaat gccaaccgca tctcgctgtt
     3241 gatcccgaaa ccgggcgaga tcgagcactg gacctatatc aatggcggcg gcggctggga
     3301 ccacccgatc cacctgcact tcgaggaagg catcaccatg aaccggggcg gggcatcctt
     3361 cccggctacc gaaaacctgg tgcgcaagga cgtctggcgc ttgcgacctt cgggtcgggt
     3421 gcagttccag gtgcagttcg gcgagtacgg cggctcctac gtcaaccact gccacaacac
     3481 cgtgcacgag gacttcgcca tgctgatgcg catccagctc ctgggcaagg cgggtacgcc
     3541 gcaggcggcc gtcacgctga cccccaatcc gagccgcgat ggcgtcacct tcacctcgcc
     3601 ggaaatcctg cgcgagggcg acccgcgcac aacccagacc gcaagttcgg gttaggccgg
     3661 tgctgtttgc cctgcgcccg ctcgcggccg ccctgcttgc ggccgccacg ctgtccccgg
     3721 ccgcggccgg ggaagcctcg cgctggggca agggctatgt tcccaatcat cccgtcgagg
     3781 cccaggacgg gcagagcttc aacttctacg acgatctgat ccaagaccgt attgccgtcg
     3841 tcagcttcat ctacacgagc tgcagcgaaa tctgcccggt ggcgaccgcc aggctggccc
     3901 agctgcagga gaagctgggc gagcacatgg ggcgcgatgt cttcttctat tccatcaccg
     3961 tcgatccgga gaccgacacg ccggcgcgca tgcagaccta tgccaagacc ttcggcgccg
     4021 gccccggctg gctgttcctg accggcaagc ccgagcacat caacgagatc cgctaccgcc
     4081 tgggcgagcg cagccgcttt ttgggcgagc accgcaacga cctcttgctc agcaacggct
     4141 tcgccggaca gtggcagcgc gattcggcga tgggcgacct cgaccgcctg atcctgacgc
     4201 tgcagtcgct caatccggcg gcattcggcg ccgcgccgct ggcgggcacc ccgcaccagc
     4261 agctggaagc catcgagctg accggcccgc cgggcgagac cctctatctc aaggcctgcg
     4321 ccgggtgcca ttcgatcggc gagggcaacc gggtcggtcc cgacctcgcc ggagtgacgc
     4381 agcgccggca gccggagtgg ctgatcaact tcatcgccaa tcccgccaag gtccgcgcca
     4441 gcaaggatcc gatcgcgctc gagcttgccg cccgcttccc gaccgtgcgc atgcccgcca
     4501 tcggcgtctc cgcacgcgag gccgccgacc tcgtcgccta catcgaggcg cgccaggccc
     4561 gcagccagcg gcgcattgcg ctagcccccc tactggcgct gacgacggcg gagggcaagc
     4621 gcctggcggc gccggagatc gagggccgcg cgctggccgt gacgttcggc ttcacgcact
     4681 gccccgacgt gtgcccgacg acgctcatgg actggagcaa cgtgctggcg agcctcgggc
     4741 ccgatggcga ccgcctcaag gtgctgttcg tctccgtcga ccccgagcgc gacacgccag
     4801 ccgtcctcaa gtcctacatg gcctccttcg acccgcgcat cacggcgctg accggcagcg
     4861 ccggcgagct ggccgcggtg gcggcggcct tcggcgcgca ctatgccaag gtgccgggcc
     4921 agcacggcag ctacaccctc gaccacacgc tcaagacctt catcctcgac gcagaggcgc
     4981 agctggccgg tgcctcggac catgccaccg acccccaaca gcgccgcgaa ctcctcaaaa
     5041 agctggccgg gcgggagtga gacttgcaag ctgaaagagg cgcgccgggc tcaacgcgtc
     5101 ttgcgcttgg gcggctcgtt cgggcgctcg ccgccctcgc cgcccgccga gcgcgggggc
     5161 aacggcagct gcaattcgtg ggtctgcccg ttgccctcga ggaccacgct cgaggtcttc
     5221 acgctcgcca gatgccaacc gttgacggtc tccccttccg gcacccactt gccgatcggc
     5281 tcgccggcga agcgaataag cgcccgtccc ggcgcgccgg cggacttgac gatgccgacg
     5341 agctgcatgc ccgtattcag cgccgccggg gcaggtccct cgaccacctc cttggtgcgt
     5401 tcgatgggtt tgcgatcggc atggaatagc ggacgctgca cggtctcctc gaacacgatc
     5461 tgaggcttgt cgtccagcgg cgtggcaagc tcgtgatccc tcgcctcctt ggcatcgacc
     5521 tggcgcgttt gcgggctgag cggggagatg tcgaccgccg tggagtagag gtgccagttg
     5581 accagcgaca gtacggcgac gccgcccagt agcgccgtca gcagcaacgg tcgggccgaa
     5641 ccgccttttt gcgcgcgtgc catgtctcat gccctcttgc gcggcgccgc gccgtagaca
     5701 tcgagacgaa cgtccaaaat gccggcgtgc tcgggatcgt gtgccatggc cgagacggtg
     5761 tgcacctgca ggccgtccac gaacaggaac ggctgtttcg attccaccgc atacaagatc
     5821 tcgcgcagtt gctcgatatt gg
//
